Sample records for bridge ca state
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3. CONTEXTUAL VIEW OF BRIDGE IN SETTING, LOOKING SOUTHWEST FROM ...
3. CONTEXTUAL VIEW OF BRIDGE IN SETTING, LOOKING SOUTHWEST FROM ELEVATED GRADE OF EUREKA SOUTHERN RAILROAD. EUREKA SOUTHERN TRUSS BRIDGE AT EXTREME LEFT, 1924 HIGHWAY BRIDGE IN CENTER, 1952 HIGHWAY BRIDGE AT RIGHT - Van Duzen River Bridge, Spanning Van Duzen River at CA State Highway 101, Alton, Humboldt County, CA
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1. CONTEXTUAL VIEW OF BRIDGE IN SETTING, LOOKING SOUTHWEST, FROM ...
1. CONTEXTUAL VIEW OF BRIDGE IN SETTING, LOOKING SOUTHWEST, FROM DOWNSTREAM. Crew, vehicles, boats, and equipment are from the California Department of Transportation's Transportation Laboratory conducting test borings for the replacement bridge. - Smith River Bridge, CA State Highway 199 Spanning Smith River, Crescent City, Del Norte County, CA
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12. DETAIL VIEW OF BRIDGE, SHOWING SPRING LINE OF SPANS ...
12. DETAIL VIEW OF BRIDGE, SHOWING SPRING LINE OF SPANS FROM CROWN OF MID-CHANNEL PIER, PAIRED COLUMNS SUPPORTING DECK, ARCHED WINDOW RAILING, LOOKING WEST-NORTHWEST FROM EUREKA SOUTHERN RAILROAD BRIDGE. CABLES VISIBLE IN BACKGROUND ARE EARTHQUAKE RESTRAINERS RETROFITTED TO 1952 HIGHWAY BRIDGE, WHICH FUNCTIONED AS DESIGNED IN APRIL 1992 TEMBLOR - Van Duzen River Bridge, Spanning Van Duzen River at CA State Highway 101, Alton, Humboldt County, CA
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5. VAL LAUNCHER BRIDGE OVER LAUNCHER SLAB TAKEN FROM RESERVOIR ...
5. VAL LAUNCHER BRIDGE OVER LAUNCHER SLAB TAKEN FROM RESERVOIR LOOKING NORTH. - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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6. Photo copy of historic photograph, ca. 1937. VIEW OF ...
6. Photo copy of historic photograph, ca. 1937. VIEW OF SOUTHERLY ELEVATION, KINGSTON STATION R.R. BRIDGE. Rhode Island Department of Transportation, Clarence Hussey Bridge Photograph Collection photograph no. 372200, Providence, Rhode Island. - Main Street Bridge, Main Street (State Route 138) over New Haven Railroad, West Kingston, Washington County, RI
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82. DETAIL OF THE MUZZLE END OF THE LAUNCHER BRIDGE ...
82. DETAIL OF THE MUZZLE END OF THE LAUNCHER BRIDGE LOOKING NORTH SHOWING THE CONNECTING BRIDGE AND TWO LAUNCHING TUBES, Date unknown, circa 1952. (Original photograph in possession of Dave Willis, San Diego, California.) - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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21. VAL, DETAIL OF MUZZLE END OF LAUNCHER BRIDGE SHOWING ...
21. VAL, DETAIL OF MUZZLE END OF LAUNCHER BRIDGE SHOWING BOTH LAUNCHER TUBES TAKEN FROM RESERVOIR LOOKING NORTH. - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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18. VAL, DETAIL OF LAUNCHER BRIDGE ALONG THE SIDE OF ...
18. VAL, DETAIL OF LAUNCHER BRIDGE ALONG THE SIDE OF THE 32' DIAMETER LAUNCHING TUBE LOOKING SOUTHWEST. - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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7. OBLIQUE VIEW OF NORTH PORTAL AND DOWNSTREAM SIDE OF ...
7. OBLIQUE VIEW OF NORTH PORTAL AND DOWNSTREAM SIDE OF BRIDGE, LOOKING WEST. Lights and illuminated sign on portal bracing were elements of an overheight load warning system designed to eliminate accidents of the type which damaged the bridge. However, the system was in place only on the north side of the bridge, controlling trucks approaching from Oregon. In theory, trucks with overheight, overwidth, or overweight loads from California would be controlled by the State's permit system. In fact, it was a 'permit' load originating in California, being hauled without the requisite permit which struck and damaged the bridge. - Smith River Bridge, CA State Highway 199 Spanning Smith River, Crescent City, Del Norte County, CA
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4. VAL PARTIAL ELEVATION SHOWING LAUNCHER BRIDGE ON SUPPORTS, LAUNCHER ...
4. VAL PARTIAL ELEVATION SHOWING LAUNCHER BRIDGE ON SUPPORTS, LAUNCHER SLAB, SUPPORT CARRIAGE, CONCRETE 'A' FRAME STRUCTURE AND CAMERA TOWER LOOKING SOUTHEAST. - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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37. BRIDGE 115, SMITH RIVER MIDDLE FORK OREGON STATE HIGHWAY ...
37. BRIDGE 1-15, SMITH RIVER MIDDLE FORK OREGON STATE HIGHWAY 199. JOSEPHINE COUNTY, OREGON. LOOKING SSW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA
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40. CAVEMAN BRIDGE, ROGUE RIVER, OREGON STATE HIGHWAY 199. GRANTS ...
40. CAVEMAN BRIDGE, ROGUE RIVER, OREGON STATE HIGHWAY 199. GRANTS PASS, JOSEPHINE COUNTY, OREGON. LOOKING S. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA
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39. CAVEMAN BRIDGE, ROGUE RIVER, OREGON STATE HIGHWAY 199. GRANTS ...
39. CAVEMAN BRIDGE, ROGUE RIVER, OREGON STATE HIGHWAY 199. GRANTS PASS, JOSEPHINE COUNTY, OREGON. LOOKING SW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA
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359. Delineator Unknown April 1935 STATE OF CALIFORNIA; DEPARTMENT OF ...
359. Delineator Unknown April 1935 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; BRIDGE RAILWAY SAN FRANCISCO LOOP; DETAILS OF VIADUCT; FINAL REPORT; DRG. NO. 92 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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STEEL ERECTION. View of upstream side of bridge, looking north ...
STEEL ERECTION. View of upstream side of bridge, looking north from the old suspension bridge at unjoined cantilever arms - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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36. MYRTLE CREEK BRIDGE, OREGON STATE HIGHWAY 199, AT END ...
36. MYRTLE CREEK BRIDGE, OREGON STATE HIGHWAY 199, AT END OF STOUT GROVE ROAD. JOSEPHINE COUNTY, OREGON LOOKING WNW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA
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71. MYRTLE CREED BRIDGE, OREGON STATE HIGHWAY 199, AT END ...
71. MYRTLE CREED BRIDGE, OREGON STATE HIGHWAY 199, AT END OF STOUT GROVE ROAD. JOSEPHINE COUNTY, OREGON. LOOKING WNW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA
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OLD SUSPENSION BRIDGE. This view appears to be looking southeast, ...
OLD SUSPENSION BRIDGE. This view appears to be looking southeast, at the downstream side of the suspension bridge, which was replaced by the South Fork Trinity River Bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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9. COMPLETED ROLLING CAMERA CAR ON RAILROAD TRACK AND BRIDGE ...
9. COMPLETED ROLLING CAMERA CAR ON RAILROAD TRACK AND BRIDGE LOOKING WEST, APRIL 26, 1948. (ORIGINAL PHOTOGRAPH IN POSSESSION OF DAVE WILLIS, SAN DIEGO, CALIFORNIA.) - Variable Angle Launcher Complex, Camera Car & Track, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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74. DETAIL VIEW OF INSIDE THE LAUNCHING BRIDGE LOOKING SOUTHWEST ...
74. DETAIL VIEW OF INSIDE THE LAUNCHING BRIDGE LOOKING SOUTHWEST SHOWING ADJUSTABLE STAIRS ON THE LEFT AND LAUNCHING TUBE ON THE RIGHT, Date unknown, circa 1948. - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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89. 22'X34' original vellum, VariableAngle Launcher 'ELEVATION OF LAUNCHER BRIDGE ...
89. 22'X34' original vellum, Variable-Angle Launcher 'ELEVATION OF LAUNCHER BRIDGE ON TEMPORARY SUPPORT' drawn at 1'=20'. (BUORD Sketch # 209786, PAPW 1932). - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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90. 22'X34' original blueprint, VariableAngle Launcher, 'FRONT ELEVATION OF LAUNCHER ...
90. 22'X34' original blueprint, Variable-Angle Launcher, 'FRONT ELEVATION OF LAUNCHER BRIDGE, CONNECTING BRIDGE AND BARGES' drawn at 1/4'=1'0'. (BUROD Sketch # 208247). - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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63. VIEW OF THE SHOOFLY BRIDGE LIFT SPAN BEING FLOATED ...
63. VIEW OF THE SHOOFLY BRIDGE LIFT SPAN BEING FLOATED INTO PLACE, LOOKING NORTH, January 29, 1935 - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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20. View to southeast. Aerial view of bridge in setting; ...
20. View to southeast. Aerial view of bridge in setting; downstream side. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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11. View to southeast. More distant overview of bridge in ...
11. View to southeast. More distant overview of bridge in setting; downstream side. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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STEEL ERECTION. View of downstream of bridge, looking southeast from ...
STEEL ERECTION. View of downstream of bridge, looking southeast from confluence of Trinity and South Fork Trinity rivers. The old suspension bridge is in background - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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LOOKING EASTSOUTHEAST. Showing downstream side of completed bridge, from confluence ...
LOOKING EAST-SOUTHEAST. Showing downstream side of completed bridge, from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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83. DETAIL OF THE MUZZLE END OF THE LAUNCHER BRIDGE ...
83. DETAIL OF THE MUZZLE END OF THE LAUNCHER BRIDGE ON TEMPORARY SUPPORTS LOOKING NORTHEAST SHOWING TWO LAUNCHING TUBES, Date unknown, circa 1950'S. (Original photograph in possession of Dave Willis, San Diego, California.) - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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356. Delineator Unknown March 1946 STATE OF CALIFORNIA; DEPARTMENT OF ...
356. Delineator Unknown March 1946 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; GENERAL DATA; PLAT III - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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STEEL ERECTION. View is to the north from the old ...
STEEL ERECTION. View is to the north from the old suspension bridge, looking at upstream side of new bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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Rhodes, Samhita S; Camara, Amadou KS; Ropella, Kristina M; Audi, Said H; Riess, Matthias L; Pagel, Paul S; Stowe, David F
2006-01-01
Background The phase-space relationship between simultaneously measured myoplasmic [Ca2+] and isovolumetric left ventricular pressure (LVP) in guinea pig intact hearts is altered by ischemic and inotropic interventions. Our objective was to mathematically model this phase-space relationship between [Ca2+] and LVP with a focus on the changes in cross-bridge kinetics and myofilament Ca2+ sensitivity responsible for alterations in Ca2+-contraction coupling due to inotropic drugs in the presence and absence of ischemia reperfusion (IR) injury. Methods We used a four state computational model to predict LVP using experimentally measured, averaged myoplasmic [Ca2+] transients from unpaced, isolated guinea pig hearts as the model input. Values of model parameters were estimated by minimizing the error between experimentally measured LVP and model-predicted LVP. Results We found that IR injury resulted in reduced myofilament Ca2+ sensitivity, and decreased cross-bridge association and dissociation rates. Dopamine (8 μM) reduced myofilament Ca2+ sensitivity before, but enhanced it after ischemia while improving cross-bridge kinetics before and after IR injury. Dobutamine (4 μM) reduced myofilament Ca2+ sensitivity while improving cross-bridge kinetics before and after ischemia. Digoxin (1 μM) increased myofilament Ca2+ sensitivity and cross-bridge kinetics after but not before ischemia. Levosimendan (1 μM) enhanced myofilament Ca2+ affinity and cross-bridge kinetics only after ischemia. Conclusion Estimated model parameters reveal mechanistic changes in Ca2+-contraction coupling due to IR injury, specifically the inefficient utilization of Ca2+ for contractile function with diastolic contracture (increase in resting diastolic LVP). The model parameters also reveal drug-induced improvements in Ca2+-contraction coupling before and after IR injury. PMID:16512898
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STARTING EXCAVATION PIER 2. This view is roughly northeast, with ...
STARTING EXCAVATION PIER 2. This view is roughly northeast, with Pier 2 on the Trinity County end of the bridge. The old suspension bridge, at upper right, was upstream of new bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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PLACING DIAGONALS IN CENTER PANEL. View is northnorthwest from the ...
PLACING DIAGONALS IN CENTER PANEL. View is north-northwest from the old suspension bridge, looking at upstream side of new bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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COMPLETED STRUCTURE. View is eastsoutheast of downstream side of bridge, ...
COMPLETED STRUCTURE. View is east-southeast of downstream side of bridge, from beyond confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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7. View to southeast. View of downstream side of bridge ...
7. View to southeast. View of downstream side of bridge from confluence of Trinity and South Fork Trinity Rivers. (90mm Lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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57. INTERIOR VIEW OF VAL BRIDGE STRUCTURE SHOWING LAUNCHING TUBE, ...
57. INTERIOR VIEW OF VAL BRIDGE STRUCTURE SHOWING LAUNCHING TUBE, STAIRS AND PORTION OF LAUNCHING DECK. NOTE SUPPORT CARRIAGE ASSEMBLY IN DISTANCE. Date unknown, circa March 1948. (Original photograph in possession of Dave Willis, San Diego, California.) - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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363. A.C.S., Delineator March 1934 STATE OF CALIFORNIA; DEPARTMENT OF ...
363. A.C.S., Delineator March 1934 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CONTRACT NO. 6A; SUPERSTRUCTURE - WEST BAY CROSSING; SAN FRANCISCO ANCHORAGE; AMERICAN BRIDGE CO.; AMBRIDGE PLANT; ORDER NO. G4866; SHEET NO E3 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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COUNTERWEIGHT ON WEST SIDE. View is to the northwest of ...
COUNTERWEIGHT ON WEST SIDE. View is to the northwest of counterweight on Humboldt County end of bridge, probably taken from the old suspension bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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77. VIEW SHOWING CONDITION OF OLD M STREET BRIDGE PIER ...
77. VIEW SHOWING CONDITION OF OLD M STREET BRIDGE PIER 1 DOLPHIN, LOOKING SOUTHEAST, March 1, 1935. (Steamer Delta King is moored at River Lines Terminal.) - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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396. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
396. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; CANTILEVER STRUCTURE; DETAILS I; DRG. NO. 68 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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393. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
393. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIER-E3; GENERAL DETAILS; DRG. NO. 47 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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397. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
397. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; CANTILEVER STRUCTURE; DETAILS II; DRG. NO. 69 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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398. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
398. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; GENERAL PLAN; TOWER E-9; DRG. NO. 59 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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364. J.G.M., Delineator February 1934 STATE OF CALIFORNIA; DEPARTMENT OF ...
364. J.G.M., Delineator February 1934 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CONTRACT NO. 6; SUPERSTRUCTURE - WEST BAY CROSSING; SAN FRANCISCO ANCHORAGE CABLE BENT CASTING; AMERICAN BRIDGE CO.; AMBRIDGE PLANT; ORDER NO. G 4852 C; SHEET NO. 100 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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378. A.C.S., Delineator March 1933 STATE OF CALIFORNIA; DEPARTMENT OF ...
378. A.C.S., Delineator March 1933 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CONTRACT NO. 6A; SUPERSTRUCTURE - WEST BAY CROSSING; YERBA BUENA ANCHORAGE & CABLE BENT. AMERICAN BRIDGE CO.; AMBRIDGE PLANT; ORDER NO. G 4866; SHEET NO. E4 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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54. VIEW SHOWING THE PLACEMENT OF SPIDER WEB BRACING, SHOOFLY ...
54. VIEW SHOWING THE PLACEMENT OF SPIDER WEB BRACING, SHOOFLY BRIDGE, January 1935 - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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381. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
381. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SUSPENDED STRUCTURE; ERECTION; DRG. NO. 43 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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369. J.R.L., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
369. J.R.L., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; CAISSON ANCHORAGE; DRG. NO. 22 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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Structure of the manganese complex in photosystem II: insights from X-ray spectroscopy.
Yachandra, Vittal K
2002-01-01
We have used Mn K-edge absorption and Kbeta emission spectroscopy to determine the oxidation states of the Mn complex in the various S states. We have started exploring the new technique of resonant inelastic X-ray scattering spectroscopy; this technique can be characterized as a Raman process that uses K-edge energies (1s to 4p, ca. 6550 eV) to obtain L-edge-like spectra (2p to 3d, ca. 650 eV). The relevance of these data to the oxidation states and structure of the Mn complex is presented. We have obtained extended X-ray absorption fine structure data from the S(0) and S(3) states and observed heterogeneity in the Mn-Mn distances leading us to conclude that there may be three rather than two di-mu-oxo-bridged units present per tetranuclear Mn cluster. In addition, we have obtained data using Ca and Sr X-ray spectroscopy that provide evidence for a heteronuclear Mn-Ca cluster. The possibility of three di-mu-oxo-bridged Mn-Mn moieties and the proximity of Ca is incorporated into developing structural models for the Mn cluster. The involvement of bridging and terminal O ligands of Mn in the mechanism of oxygen evolution is discussed in the context of our X-ray spectroscopy results. PMID:12437873
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380. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...
380. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CABLES & ATTACHMENTS WEST BAY CROSSING; CABLE BANDS; CONTRACT NO. 6A; DRAWING NO. 3 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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389. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
389. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SUSPENDED STRUCTURE; TYPICAL DETAILS; DRG. NO. 42 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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366. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
366. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SAN FRANCISCO CABLE BENT; DRG. NO. 33 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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382. J.R.L., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
382. J.R.L., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; TOWERS; GENERAL ELEVATIONS & SECTIONS; DRG. NO. 28 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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383. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
383. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; TOWERS; TYPICAL BASE DETAILS; DRG. NO. 29 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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399. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
399. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; 504FOOT SPANS; TYPICAL DETAILS; DRG. NO. 64 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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360. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
360. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SAN FRANCISCO ANCHORAGE; TYPICAL SECTIONS; DRG. NO. 13 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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391. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
391. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIER E1; GENERAL DETAILS; DRG. NO. 45 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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395. J.L.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
395. J.L.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; TOWER E-4; GENERAL PLAN; DRG. NO. 58 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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385. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...
385. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; ROCKER POSTS AND BEARING; CONTRACT NO. 6; DRAWINGS NO. 42 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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361. W.J.M., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
361. W.J.M., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SAN FRANCISCO ANCHORAGE; TYPICAL SECTIONS; DRG. NO. 14 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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386. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
386. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; TOWERS; TYPICAL TOP DETAILS; DRG. NO. 31 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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384. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
384. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; TOWERS; BRACING AND ROCKER BRACKET DETAILS; DRG. NO. 30 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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379. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...
379. Delineator Unknown December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CABLES AND ATTACHMENTS - WEST BAY CROSSING; SPLAY CASTINGS; CONTRACT NO. 6A; DRAWING NO. 4 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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373. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
373. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; PIER W-4; DETAILS OF A-FRAME; DRG. NO. 19 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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370. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
370. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; PIER W-4; PLANS AND ELEVATIONS; DRG. NO. 17 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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400. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
400. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIER E-6 TO E-23; TYPICAL DETAILS; DRG. NO. 52 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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372. J.W.M., Delineator August 1934 STATE OF CALIFORNIA; DEPARTMENT OF ...
372. J.W.M., Delineator August 1934 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; CONTRACT NO. 6; SUPERSTRUCTURE - WEST BAY CROSSING; OUTSIDE ELEVATION OF HOUSING; CENTER ANCHORAGE - PIER NO. 4; AMERICAN BRIDGE CO.; AMBRIDGE PLANT; ORDER NO. G 4854-XI; SHEET NO. E8 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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Aftermath. The remains of the southwest end of the bridge ...
Aftermath. The remains of the southwest end of the bridge lie next to the southwest pier. View is south-southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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Dust trailing from the top chord, the bridge falls toward ...
Dust trailing from the top chord, the bridge falls toward the river, as the southwest end (right) falls first. View southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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COUNTERWEIGHT FOOTINGS ON EAST SIDE. View is to the north ...
COUNTERWEIGHT FOOTINGS ON EAST SIDE. View is to the north from the old suspension bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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13. View to northeast. View along centerline from Humboldt County ...
13. View to northeast. View along centerline from Humboldt County side of bridge. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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12. View to southwest. View along centerline from Trinity County ...
12. View to southwest. View along centerline from Trinity County side of bridge. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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392. J.R.L., Delineator August 1933 STATE OF CALIFORNIA; DEPARTMENT OF ...
392. J.R.L., Delineator August 1933 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - EAST BAY CROSSING; PIER El; ANCHORAGE STEEL WORK; CONTRACT NO. 7; SUP. DRAWING NO. 72A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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377. F.A.N. and Q.E.D., Delineators Date Unknown STATE OF CALIFORNIA; ...
377. F.A.N. and Q.E.D., Delineators Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; YERBA BUENA CABLE BENT; DRG. NO. 34 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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388. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...
388. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; SUSPENDED STRUCTURE; SIDE SPAN TRUSSES AT ANCHORAGES; CONTRACT NO. 6; DRAWING NO. 40 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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390. Delineator Unknown October 1933 STATE OF CALIFORNIA; DEPARTMENT OF ...
390. Delineator Unknown October 1933 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - EAST BAY CROSSING; SPANS YBI TO El - E9 TO El ; TYPICAL CROSSSECTION; CONTRACT NO. 7; SUP. DRAWING NO. 82 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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PIER 2. View is to the northeast, looking from Pier ...
PIER 2. View is to the northeast, looking from Pier 1 toward Pier 2 from beneath completed bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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3. View to southwest. Oblique view of downstream side of ...
3. View to southwest. Oblique view of downstream side of bridge and west pier. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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2. View to east. Oblique view of downstream side of ...
2. View to east. Oblique view of downstream side of bridge and east pier. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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9. View to northeast. Oblique view of upstream side of ...
9. View to northeast. Oblique view of upstream side of bridge from approximately deck level. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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PLACING TOP CHORD CENTER PANEL. View to the northwest from ...
PLACING TOP CHORD CENTER PANEL. View to the northwest from the old suspension bridge. Chord members in place - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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19. ...INTAKE STRUCTURE AND PIER FOR SERVICE BRIDGE NEARING COMPLETION. ...
19. ...INTAKE STRUCTURE AND PIER FOR SERVICE BRIDGE NEARING COMPLETION. Volume XVI, No. 14, September 29, 1939. - Prado Dam, Outlet Works, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA
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43. OUTLET WORKS: SERVICE BRIDGE PLAN AND ELEVATION. Sheet ...
43. OUTLET WORKS: SERVICE BRIDGE - PLAN AND ELEVATION. Sheet 37, August 20, 1938. File no. SA 121/76. - Prado Dam, Outlet Works, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA
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45. OUTLET WORKS: SERVICE BRIDGE BEARING DETAILS. Sheet 39, ...
45. OUTLET WORKS: SERVICE BRIDGE - BEARING DETAILS. Sheet 39, August 20, 1938. File no. SA 121/78. - Prado Dam, Outlet Works, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA
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44. OUTLET WORKS: SERVICE BRIDGE GIRDER DETAILS. Sheet 38, ...
44. OUTLET WORKS: SERVICE BRIDGE - GIRDER DETAILS. Sheet 38, August 20, 1938. File no. SA 121/77. - Prado Dam, Outlet Works, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA
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1. HEALDSBURG BRIDGE, OLD HIGHWAY 101, ACROSS THE RUSSIAN RIVER. ...
1. HEALDSBURG BRIDGE, OLD HIGHWAY 101, ACROSS THE RUSSIAN RIVER. HEALDSBURG, MEDDOCINO COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA
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With deck removed and critical members severed, the bridge begins ...
With deck removed and critical members severed, the bridge begins to succumb to tractors, pulling cables attached to its downstream side. View is to the southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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LOWER CHORD ERECTION. View is to northnorthwest from the old ...
LOWER CHORD ERECTION. View is to north-northwest from the old suspension bridge. Chord members suspended from jigger stick - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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4. View to westsouthwest. Oblique view of upstream side of ...
4. View to west-southwest. Oblique view of upstream side of bridge from approximately deck level. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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401. J.W.P.C., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
401. J.W.P.C., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIERS E6 TO E-22; SEQUENCE OF OPERATION; DETAILS OF EQUIPMENT; DRG. NO. 53 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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413. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
413. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; DIVISION OF SAN FRANCISCO BAY TOLL CROSSINGS; SAN FRANCISCO OAKLAND BAY BRIDGE RECONSTRUCTION; STEEL WORK - WEST BAY; CONTINUOUS SPANS - LONGITUDINAL GIRDERS; SHEET NO. 18; DRAWING NO. C-4028-18R - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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387. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...
387. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; TOWERS 2, 3, 5 & 6; BRACING DETAILS - LOWER DECK; CONTRACT NO. 6; DRAWING NO. 27 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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394. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
394. F.A.N., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; PIERS E-2, E-3, E-4, E-5; PLANT LAYOUT AND CAISSON ANCHORAGES; DRG. NO.51 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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367. J.W.G., Delineator August 1933 STATE OF CALIFORNIA; DEPARTMENT OF ...
367. J.W.G., Delineator August 1933 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; PIER NO. 5; GENERAL PLAN & ELEVATION; CONTRACT NO. 2; SUP. DRAWING NO. 10A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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414. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
414. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; DIVISION OF SAN FRANCISCO BAY TOLL CROSSINGS; SAN FRANCISCO OAKLAND BAY BRIDGE RECONSTRUCTION; STEEL WORK - WEST BAY; TYPICAL SECTIONS; SHEET NO. 5; DRAWING NO. C-4028-5R - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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28. 'TOWER DESIGN NO. 11, ADAPTED FROM NO. 9,' drawn ...
28. 'TOWER DESIGN NO. 11, ADAPTED FROM NO. 9,' drawn by project architect Alfred Eichler, undated, ca. 1934. - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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371. A.J.M. and D.L.S., Delineators April 1934. STATE OF CALIFORNIA; ...
371. A.J.M. and D.L.S., Delineators April 1934. STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; PIER NO. 4; VERTICAL SECTIONS; CONTRACT NO. 2; SUP. DRAWING NO. 17A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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Scaling law on formation and rupture of a dynamical liquid bridge
NASA Astrophysics Data System (ADS)
Zhang, Huang; Zhang, Zehao; Liu, Qianfeng; Li, Shuiqing; Department of Thermal Engineering, Tsinghua University Collaboration; Institute of Nuclear Energy; Technology, Tsinghua University Collaboration
2017-11-01
The formation and breakup of a pendular liquid bridge in dynamic state is investigated experimentally. The experimental setup arises from a system to measure the coefficient of restitution (COR) of a glass sphere impacting and bouncing on a wetted surface. We compare the effect of surface tension and gravity on the liquid bridge rupture by the capillary length κ-1. For water and liquid 1 (50% water mixed with 50% glycerol), the gravity is dominant on the liquid bridge breakup. And we find that the rupture distance is in good linear trend with the non-dimensional number G by the scaling law analysis. Further, for liquid 2 (25% water mixed with 75% glycerol) that is relatively high viscous, the linear changing of the rupture distance with the capillary number Ca is found. The relation of the rupture distance with G and Ca would be helpful in understanding the complex behavior of the dynamical liquid bridge. This work was funded by the Major State Basic Research Development Program of China (Grant No. 2016YFC0203705) and the China Postdoctoral Science Foundation (Grant No. 2016M601024).
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5. VAL CAMERA CAR, DETAIL OF HOIST AT SIDE OF ...
5. VAL CAMERA CAR, DETAIL OF HOIST AT SIDE OF BRIDGE AND ENGINE CAR ON TRACKS, LOOKING NORTHEAST. - Variable Angle Launcher Complex, Camera Car & Track, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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32. VAL, DETAIL SHOWING LOADING PLATFORM, PROJECTILE LOADING CAR, LAUNCHER ...
32. VAL, DETAIL SHOWING LOADING PLATFORM, PROJECTILE LOADING CAR, LAUNCHER SLAB AND UNDERSIDE OF LAUNCHER BRIDGE LOOKING SOUTHWEST. - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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Oblique perspective, due east by 70 degrees. Note concrete pier, ...
Oblique perspective, due east by 70 degrees. Note concrete pier, added CA. 1930's. Other piers and abutments are heavily mortared rubble stone. - Watson Mill Bridge, Spanning South Fork Broad River, Watson Mill Road, Watson Mill Bridge State Park, Comer, Madison County, GA
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PLACING TOP CHORD CENTER PANEL. View is to the northwest ...
PLACING TOP CHORD CENTER PANEL. View is to the northwest from the old suspension bridge. Chord members being moved into place by jigger stick and highline - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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12. VIEW, LOOKING NORTHWEST, ALONG CENTERLINE FROM NORTH CANTILEVER TOWER ...
12. VIEW, LOOKING NORTHWEST, ALONG CENTERLINE FROM NORTH CANTILEVER TOWER TOWARD SOUTH PORTAL. As with previous photo, note vertical displacement in deck. - Smith River Bridge, CA State Highway 199 Spanning Smith River, Crescent City, Del Norte County, CA
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LOOKING EAST. View is to the northeast along centerline of ...
LOOKING EAST. View is to the northeast along centerline of deck, looking from Humboldt County toward Trinity County. The old suspension bridge is still in place at right - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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415. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...
415. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; DIVISION OF SAN FRANCISCO BAY TOLL CROSSINGS; SAN FRANCISCO OAKLAND BAY BRIDGE RECONSTRUCTION; STEEL WORK - WEST BAY; Y.B. ANCHORAGE - FLOOR BEAMS YA-5 AND BENT 3; SHEET NO. 22; DRAWING NO. C-4028-22R - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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With water still in midflight, the northeast end of the ...
With water still in mid-flight, the northeast end of the bridge (left) nears total collapse. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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79. VIEW OF VAL FIRING RANGE LOOKING SOUTHWEST SHOWING LAUNCHER ...
79. VIEW OF VAL FIRING RANGE LOOKING SOUTHWEST SHOWING LAUNCHER BRIDGE, BARGES, SONAR BUOY RANGE AND MORRIS DAM IN BACKGROUND, June 10, 1948. - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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37. ALTERNATE DESIGN, SIMILAR TO THAT ULTIMATELY SELECTED, BUT USING ...
37. ALTERNATE DESIGN, SIMILAR TO THAT ULTIMATELY SELECTED, BUT USING STEPPED TOWERS, AND WITH PYLONS CAPPED BY LANTERNS Pen-and-ink drawing by project architect Alfred Eichler, ca. 1934. - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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30. VAL LOOKING DOWN THE LAUNCHER SLAB STAIRS AT THE ...
30. VAL LOOKING DOWN THE LAUNCHER SLAB STAIRS AT THE PROJECTILE LOADING CAR AND LOADING PLATFORM ADJACENT TO THE PROJECTILE LOADING DECK AND LAUNCHER BRIDGE. - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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1. LOOKING NORTH AT SOUTH FRONT OF CONTROL HOUSE. ALSO ...
1. LOOKING NORTH AT SOUTH FRONT OF CONTROL HOUSE. ALSO SHOWN ARE THE CONTROL TOWER, THE SERVICE BRIDGE, AND THE SERVICE BRIDGE PIER. SNOW COVERED MT. BALDY IS VISIBLE IN THE DISTANCE. - Prado Dam, Outlet Works, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA
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CO-Bridged H-Cluster Intermediates in the Catalytic Mechanism of [FeFe]-Hydrogenase CaI
Ratzloff, Michael W.; Artz, Jacob H.; Mulder, David W.; ...
2018-05-23
The [FeFe]-hydrogenases ([FeFe] H 2ases) catalyze reversible H 2 activation at the H-cluster, which is composed of a [4Fe-4S] H subsite linked by a cysteine thiolate to a bridged, organometallic [2Fe-2S] ([2Fe] H) subsite. Profoundly different geometric models of the H-cluster redox states that orchestrate the electron/proton transfer steps of H 2 bond activation have been proposed. We have examined this question in the [FeFe] H 2ase I from Clostridium acetobutylicum (CaI) by Fourier-transform infrared (FTIR) spectroscopy with temperature annealing and H/D isotope exchange to identify the relevant redox states and define catalytic transitions. One-electron reduction of H ox ledmore » to formation of H redH + ([4Fe-4S] H 2+-Fe I-Fe I) and H red' ([4Fe-4S] H 1+-Fe II-Fe I), with both states characterized by low frequency μ-CO IR modes consistent with a fully bridged [2Fe] H. Similar μ-CO IR modes were also identified for H redH + of the [FeFe] H 2ase from Chlamydomonas reinhardtii (CrHydA1). The CaI proton-transfer variant C298S showed enrichment of an H/D isotope-sensitive μ-CO mode, a component of the hydride bound H-cluster IR signal, H hyd. Equilibrating CaI with increasing amounts of NaDT, and probed at cryogenic temperatures, showed H redH + was converted to H hyd. Over an increasing temperature range from 10 to 260 K catalytic turnover led to loss of Hhyd and appearance of H ox, consistent with enzymatic turnover and H 2 formation. The results show for CaI that the μ-CO of [2Fe] H remains bridging for all of the 'H red' states and that H redH + is on pathway to H hyd and H 2 evolution in the catalytic mechanism. Here, this provides a blueprint for designing small molecule catalytic analogs« less
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CO-Bridged H-Cluster Intermediates in the Catalytic Mechanism of [FeFe]-Hydrogenase CaI
DOE Office of Scientific and Technical Information (OSTI.GOV)
Ratzloff, Michael W.; Artz, Jacob H.; Mulder, David W.
The [FeFe]-hydrogenases ([FeFe] H 2ases) catalyze reversible H 2 activation at the H-cluster, which is composed of a [4Fe-4S] H subsite linked by a cysteine thiolate to a bridged, organometallic [2Fe-2S] ([2Fe] H) subsite. Profoundly different geometric models of the H-cluster redox states that orchestrate the electron/proton transfer steps of H 2 bond activation have been proposed. We have examined this question in the [FeFe] H 2ase I from Clostridium acetobutylicum (CaI) by Fourier-transform infrared (FTIR) spectroscopy with temperature annealing and H/D isotope exchange to identify the relevant redox states and define catalytic transitions. One-electron reduction of H ox ledmore » to formation of H redH + ([4Fe-4S] H 2+-Fe I-Fe I) and H red' ([4Fe-4S] H 1+-Fe II-Fe I), with both states characterized by low frequency μ-CO IR modes consistent with a fully bridged [2Fe] H. Similar μ-CO IR modes were also identified for H redH + of the [FeFe] H 2ase from Chlamydomonas reinhardtii (CrHydA1). The CaI proton-transfer variant C298S showed enrichment of an H/D isotope-sensitive μ-CO mode, a component of the hydride bound H-cluster IR signal, H hyd. Equilibrating CaI with increasing amounts of NaDT, and probed at cryogenic temperatures, showed H redH + was converted to H hyd. Over an increasing temperature range from 10 to 260 K catalytic turnover led to loss of Hhyd and appearance of H ox, consistent with enzymatic turnover and H 2 formation. The results show for CaI that the μ-CO of [2Fe] H remains bridging for all of the 'H red' states and that H redH + is on pathway to H hyd and H 2 evolution in the catalytic mechanism. Here, this provides a blueprint for designing small molecule catalytic analogs« less
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CO-Bridged H-Cluster Intermediates in the Catalytic Mechanism of [FeFe]-Hydrogenase CaI.
Ratzloff, Michael W; Artz, Jacob H; Mulder, David W; Collins, Reuben T; Furtak, Thomas E; King, Paul W
2018-06-20
The [FeFe]-hydrogenases ([FeFe] H 2 ases) catalyze reversible H 2 activation at the H-cluster, which is composed of a [4Fe-4S] H subsite linked by a cysteine thiolate to a bridged, organometallic [2Fe-2S] ([2Fe] H ) subsite. Profoundly different geometric models of the H-cluster redox states that orchestrate the electron/proton transfer steps of H 2 bond activation have been proposed. We have examined this question in the [FeFe] H 2 ase I from Clostridium acetobutylicum (CaI) by Fourier-transform infrared (FTIR) spectroscopy with temperature annealing and H/D isotope exchange to identify the relevant redox states and define catalytic transitions. One-electron reduction of H ox led to formation of H red H + ([4Fe-4S] H 2+ -Fe I -Fe I ) and H red ' ([4Fe-4S] H 1+ -Fe II -Fe I ), with both states characterized by low frequency μ-CO IR modes consistent with a fully bridged [2Fe] H . Similar μ-CO IR modes were also identified for H red H + of the [FeFe] H 2 ase from Chlamydomonas reinhardtii (CrHydA1). The CaI proton-transfer variant C298S showed enrichment of an H/D isotope-sensitive μ-CO mode, a component of the hydride bound H-cluster IR signal, H hyd . Equilibrating CaI with increasing amounts of NaDT, and probed at cryogenic temperatures, showed H red H + was converted to H hyd . Over an increasing temperature range from 10 to 260 K catalytic turnover led to loss of H hyd and appearance of H ox , consistent with enzymatic turnover and H 2 formation. The results show for CaI that the μ-CO of [2Fe] H remains bridging for all of the "H red " states and that H red H + is on pathway to H hyd and H 2 evolution in the catalytic mechanism. These results provide a blueprint for designing small molecule catalytic analogs.
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11. VIEW, LOOKING SOUTHEAST, ALONG CENTERLINE FROM SOUTH CANTILEVER TOWARD ...
11. VIEW, LOOKING SOUTHEAST, ALONG CENTERLINE FROM SOUTH CANTILEVER TOWARD TOWARD NORTH PORTAL. Note vertical displacement in deck caused by partial collapse at point of connection between south cantilever arm and suspended span. - Smith River Bridge, CA State Highway 199 Spanning Smith River, Crescent City, Del Norte County, CA
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28. VIEW TO NORTHEAST. VIEW OVER TOP OF TRUSS FROM ...
28. VIEW TO NORTHEAST. VIEW OVER TOP OF TRUSS FROM CONTROL CABIN DECK. Photographer unknown, August 1947 (Note that frame for electrical power cables is still in place, though the bridge was converted to hand operation almost ten years earlier.) - Gianella Bridge, Spanning Sacramento River at State Highway 32, Hamilton City, Glenn County, CA
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60. C.J.T., photographer December 23, 1955 KLAMATH RIVER BRIDGE, DEL ...
60. C.J.T., photographer December 23, 1955 KLAMATH RIVER BRIDGE, DEL NORTE COUNTY, SECTION A, HIGHWAY 1. DN-1-A #538, KLAMATH RIVER BR. FROM SO. END, 12/23/55, C.J.T. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA
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33 CFR 165.1110 - Security Zone: Coronado Bay Bridge, San Diego, CA.
Code of Federal Regulations, 2011 CFR
2011-07-01
... Bridge, San Diego, CA. 165.1110 Section 165.1110 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.1110 Security Zone: Coronado Bay Bridge, San Diego, CA. (a) Location. All navigable waters of San... pilings of the Coronado Bay Bridge. These security zones will not restrict the main navigational channel...
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33 CFR 165.1110 - Security Zone: Coronado Bay Bridge, San Diego, CA.
Code of Federal Regulations, 2010 CFR
2010-07-01
... Bridge, San Diego, CA. 165.1110 Section 165.1110 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.1110 Security Zone: Coronado Bay Bridge, San Diego, CA. (a) Location. All navigable waters of San... pilings of the Coronado Bay Bridge. These security zones will not restrict the main navigational channel...
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81. VIEW OF VAL LOOKING NORTH AS SEEN FROM THE ...
81. VIEW OF VAL LOOKING NORTH AS SEEN FROM THE RESERVOIR SHOWING TWO LAUNCHING TUBES ON THE LAUNCHER BRIDGE, Date unknown, circa 1952. (Original photograph in possession of Dave Willis, San Diego, California.) - Variable Angle Launcher Complex, Variable Angle Launcher, CA State Highway 39 at Morris Reservior, Azusa, Los Angeles County, CA
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77 FR 70891 - Safety Zone; Bay Bridge Construction, San Francisco Bay, San Francisco, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2012-11-28
...-AA00 Safety Zone; Bay Bridge Construction, San Francisco Bay, San Francisco, CA AGENCY: Coast Guard... the navigable waters of the San Francisco Bay near Yerba Buena Island, CA in support of the Bay Bridge... construction of the Bay Bridge, the safety zone is necessary to provide for the safety of mariners transiting...
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25. VIEW, LOOKING SOUTHWEST INSIDE TRANSFORMER ROOM, SHOWING TRANSFORMERS AND ...
25. VIEW, LOOKING SOUTHWEST INSIDE TRANSFORMER ROOM, SHOWING TRANSFORMERS AND KNIFE SWITCHES - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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26. VIEW, LOOKING NORTHWEST INSIDE TRANSFORMER ROOM, SHOWING OIL FILLED ...
26. VIEW, LOOKING NORTHWEST INSIDE TRANSFORMER ROOM, SHOWING OIL- FILLED TRANSFORMER POTS - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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17. DETAIL VIEW OF CUPOLA ATOP OPERATOR'S CABIN WHICH MOUNTS ...
17. DETAIL VIEW OF CUPOLA ATOP OPERATOR'S CABIN WHICH MOUNTS SIGNAL HORNS, WEATHER VANE - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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Kheradmandan, S; Koutayas, S O; Bernhard, M; Strub, J R
2001-04-01
The purpose of this study was to evaluate the fracture strength of four different types of anterior 3-unit bridges after thermo-mechanical fatigue in a dual-axis chewing simulator. Sixty-four human maxillary incisors were prepared and 32 bridges fabricated. The four groups of eight bridges each were - (GC): AGC(R) galvano-ceramic bridges (CA): Celay(R) In-Ceram(R) Alumina bridges (E2): heat-pressed lithium disilicate glass-ceramic bridges and (CM) ceramo-metal (control). Fracture loads were recorded after a dual-axis chewing simulator and in a universal testing machine. The survival rate after 5 years for the CM and the GC groups was 100%, for the E2 group 75% and for the CA group 37.5% (Kaplan-Mayer analysis). The mean fracture strengths (N) were 681.52 +/- 151.90 (CM); 397.71 +/- 59.02 (GC); 292.92 +/- 46.45 (E2) and 239.95 +/- 33.39 (CA), respectively. The log-rank test showed a significant difference between the CA and the GC or the CA and the CM groups. No significant differences between the E2 and the CA or the E2 and the GC groups were found. The study indicates that heat-pressed lithium disilicate glass-ceramic and AGC(R) galvano-ceramic bridges are alternatives to ceramo-metal 3-unit anterior bridges.
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23. VIEW, LOOKING SOUTH, SHOWING GASOLINEPOWERED EMERGENCY GENERATOR, WITH MAIN ...
23. VIEW, LOOKING SOUTH, SHOWING GASOLINE-POWERED EMERGENCY GENERATOR, WITH MAIN TRANSMISSION AT LEFT FOREGROUND - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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78. VIEW SHOWING PLACEMENT OF LIFE SPAN SHOE ON PIER ...
78. VIEW SHOWING PLACEMENT OF LIFE SPAN SHOE ON PIER 6, LOOKING NORTH, March 5, 1935 - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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Force transients and minimum cross-bridge models in muscular contraction.
Kawai, Masataka; Halvorson, Herbert R
2007-01-01
Two- and three-state cross-bridge models are considered and examined with respect to their ability to predict three distinct phases of the force transients that occur in response to step change in muscle fiber length. Particular attention is paid to satisfying the Le Châtelier-Brown Principle. This analysis shows that the two-state model can account for phases 1 and 2 of a force transient, but is barely adequate to account for phase 3 (delayed force) unless a stretch results in a sudden increase in the number of cross-bridges in the detached state. The three-state model (A-->B-->C-->A) makes it possible to account for all three phases if we assume that the A-->B transition is fast (corresponding to phase 2), the B-->A transition is of intermediate speed (corresponding to phase 3), and the C-->A transition is slow; in such a scenario, states A and C can support or generate force (high force states) but state B cannot (detached, or low-force state). This model involves at least one ratchet mechanism. In this model, force can be generated by either of two transitions: B-->A or B-->C. To determine which of these is the major force-generating step that consumes ATP and transduces energy, we examine the effects of ATP, ADP, and phosphate (Pi) on force transients. In doing so, we demonstrate that the fast transition (phase 2) is associated with the nucleotide-binding step, and that the intermediate-speed transition (phase 3) is associated with the Pi-release step. To account for all the effects of ligands, it is necessary to expand the three-state model into a six-state model that includes three ligand-bound states. The slowest phase of a force transient (phase 4) cannot be explained by any of the models described unless an additional mechanism is introduced. Here we suggest a role of series compliance to account for this phase, and propose a model that correlates the slowest step of the cross-bridge cycle (transition C-->A) to: phase 4 of step analysis, the rate constant k(tr) of the quick-release and restretch experiment, and the rate constant k(act) for force development time course following Ca(2+) activation.
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Young, Iain S; Harwood, Claire L; Rome, Lawrence C
2003-10-01
Because the major processes involved in muscle contraction require rapid utilization of ATP, measurement of ATP utilization can provide important insights into the mechanisms of contraction. It is necessary, however, to differentiate between the contribution made by cross-bridges and that of the sarcoplasmic reticulum (SR) Ca2+ pumps. Specific and potent SR Ca2+ pump blockers have been used in skinned fibers to permit direct measurement of cross-bridge ATP utilization. Up to now, there was no analogous cross-bridge blocker. Recently, N-benzyl-p-toluene sulfonamide (BTS) was found to suppress force generation at micromolar concentrations. We tested whether BTS could be used to block cross-bridge ATP utilization, thereby permitting direct measurement of SR Ca2+ pump ATP utilization in saponin-skinned fibers. At 25 microM, BTS virtually eliminates force and cross-bridge ATP utilization (both <4% of control value). By taking advantage of the toadfish swimbladder muscle's unique right shift in its force-Ca2+ concentration ([Ca2+]) relationship, we measured SR Ca2+ pump ATP utilization in the presence and absence of BTS. At 25 microM, BTS had no effect on SR pump ATP utilization. Hence, we used BTS to make some of the first direct measurements of ATP utilization of intact SR over a physiological range of [Ca2+]at 15 degrees C. Curve fits to SR Ca2+ pump ATP utilization vs. pCa indicate that they have much lower Hill coefficients (1.49) than that describing cross-bridge force generation vs. pCa (approximately 5). Furthermore, we found that BTS also effectively eliminates force generation in bundles of intact swimbladder muscle, suggesting that it will be an important tool for studying integrated SR function during normal motor behavior.
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A mathematical model of airway and pulmonary arteriole smooth muscle.
Wang, Inga; Politi, Antonio Z; Tania, Nessy; Bai, Yan; Sanderson, Michael J; Sneyd, James
2008-03-15
Airway hyperresponsiveness is a major characteristic of asthma and is believed to result from the excessive contraction of airway smooth muscle cells (SMCs). However, the identification of the mechanisms responsible for airway hyperresponsiveness is hindered by our limited understanding of how calcium (Ca2+), myosin light chain kinase (MLCK), and myosin light chain phosphatase (MLCP) interact to regulate airway SMC contraction. In this work, we present a modified Hai-Murphy cross-bridge model of SMC contraction that incorporates Ca2+ regulation of MLCK and MLCP. A comparative fit of the model simulations to experimental data predicts 1), that airway and arteriole SMC contraction is initiated by fast activation by Ca2+ of MLCK; 2), that airway SMC, but not arteriole SMC, is inhibited by a slower activation by Ca2+ of MLCP; and 3), that the presence of a contractile agonist inhibits MLCP to enhance the Ca2+ sensitivity of airway and arteriole SMCs. The implication of these findings is that murine airway SMCs exploit a Ca2+-dependent mechanism to favor a default state of relaxation. The rate of SMC relaxation is determined principally by the rate of release of the latch-bridge state, which is predicted to be faster in airway than in arteriole. In addition, the model also predicts that oscillations in calcium concentration, commonly observed during agonist-induced smooth muscle contraction, cause a significantly greater contraction than an elevated steady calcium concentration.
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2. HISTORIC PHOTOGRAPH, VIEW OF GUINEA ROAD BRIDGE,CA. 1940. COLLECTION ...
2. HISTORIC PHOTOGRAPH, VIEW OF GUINEA ROAD BRIDGE,CA. 1940. COLLECTION CONNECTICUT DEPARTMENT OF TRANSPORTATION. - Merritt Parkway, Guinea Road Bridge, Spanning Merritt Parkway, Stamford, Fairfield County, CT
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CANVAS FINISH TO REMOVE FLOAT MARKS. View is to the ...
CANVAS FINISH TO REMOVE FLOAT MARKS. View is to the northwest of deck finishing operations - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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14. View to southwest. View through truss along centerline from ...
14. View to southwest. View through truss along centerline from below deck. (65mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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JIGGER STICK USED IN ERECTION. Shown on highline, direction of ...
JIGGER STICK USED IN ERECTION. Shown on highline, direction of view is probably to the north - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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The interdependence of Ca2+ activation, sarcomere length, and power output in the heart.
McDonald, Kerry S
2011-07-01
Myocardium generates power to perform external work on the circulation; yet, many questions regarding intermolecular mechanisms regulating power output remain unresolved. Power output equals force × shortening velocity, and some interesting new observations regarding control of these two factors have arisen. While it is well established that sarcomere length tightly controls myocyte force, sarcomere length-tension relationships also appear to be markedly modulated by PKA-mediated phosphorylation of myofibrillar proteins. Concerning loaded shortening, historical models predict independent cross-bridge mechanics; however, it seems that the mechanical state of one population of cross-bridges affects the activity of other cross-bridges by, for example, recruitment of cross-bridges from the non-cycling pool to the cycling force-generating pool during submaximal Ca(2+) activation. This is supported by the findings that Ca(2+) activation levels, myofilament phosphorylation, and sarcomere length are all modulators of loaded shortening and power output independent of their effects on force. This fine tuning of power output probably helps optimize myocardial energetics and to match ventricular supply with peripheral demand; yet, the discernment of the chemo-mechanical signals that modulate loaded shortening needs further clarification since power output may be a key convergent point and feedback regulator of cytoskeleton and cellular signals that control myocyte growth and survival.
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33 CFR 165.1110 - Security Zone: Coronado Bay Bridge, San Diego, CA.
Code of Federal Regulations, 2013 CFR
2013-07-01
... Bridge, San Diego, CA. 165.1110 Section 165.1110 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.1110 Security Zone: Coronado Bay Bridge, San Diego, CA. (a) Location. All navigable waters of San Diego Bay, from the surface to the sea floor, within 25 yards of all piers, abutments, fenders and...
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33 CFR 165.1110 - Security Zone: Coronado Bay Bridge, San Diego, CA.
Code of Federal Regulations, 2014 CFR
2014-07-01
... Bridge, San Diego, CA. 165.1110 Section 165.1110 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.1110 Security Zone: Coronado Bay Bridge, San Diego, CA. (a) Location. All navigable waters of San Diego Bay, from the surface to the sea floor, within 25 yards of all piers, abutments, fenders and...
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33 CFR 165.1110 - Security Zone: Coronado Bay Bridge, San Diego, CA.
Code of Federal Regulations, 2012 CFR
2012-07-01
... Bridge, San Diego, CA. 165.1110 Section 165.1110 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.1110 Security Zone: Coronado Bay Bridge, San Diego, CA. (a) Location. All navigable waters of San Diego Bay, from the surface to the sea floor, within 25 yards of all piers, abutments, fenders and...
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17. View to west. Detail, connection point L2 (see plans), ...
17. View to west. Detail, connection point L2 (see plans), from below deck. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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A Mathematical Model of Airway and Pulmonary Arteriole Smooth Muscle
Wang, Inga; Politi, Antonio Z.; Tania, Nessy; Bai, Yan; Sanderson, Michael J.; Sneyd, James
2008-01-01
Airway hyperresponsiveness is a major characteristic of asthma and is believed to result from the excessive contraction of airway smooth muscle cells (SMCs). However, the identification of the mechanisms responsible for airway hyperresponsiveness is hindered by our limited understanding of how calcium (Ca2+), myosin light chain kinase (MLCK), and myosin light chain phosphatase (MLCP) interact to regulate airway SMC contraction. In this work, we present a modified Hai-Murphy cross-bridge model of SMC contraction that incorporates Ca2+ regulation of MLCK and MLCP. A comparative fit of the model simulations to experimental data predicts 1), that airway and arteriole SMC contraction is initiated by fast activation by Ca2+ of MLCK; 2), that airway SMC, but not arteriole SMC, is inhibited by a slower activation by Ca2+ of MLCP; and 3), that the presence of a contractile agonist inhibits MLCP to enhance the Ca2+ sensitivity of airway and arteriole SMCs. The implication of these findings is that murine airway SMCs exploit a Ca2+-dependent mechanism to favor a default state of relaxation. The rate of SMC relaxation is determined principally by the rate of release of the latch-bridge state, which is predicted to be faster in airway than in arteriole. In addition, the model also predicts that oscillations in calcium concentration, commonly observed during agonist-induced smooth muscle contraction, cause a significantly greater contraction than an elevated steady calcium concentration. PMID:18065464
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15. View to northeast. View along centerline from below deck; ...
15. View to northeast. View along centerline from below deck; detail of bent and floor beams. (65mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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16. View to southsouthwest. Detail, connection point U1 (see plans), ...
16. View to south-southwest. Detail, connection point U1 (see plans), from below deck. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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Mickelson, Alexis V; Chandra, Murali
2017-12-01
The central region of cardiac troponin T (TnT) is important for modulating the dynamics of muscle length-mediated cross-bridge recruitment. Therefore, hypertrophic cardiomyopathy mutations in the central region may affect cross-bridge recruitment dynamics to alter myofilament Ca 2+ sensitivity and length-dependent activation of cardiac myofilaments. Given the importance of the central region of TnT for cardiac contractile dynamics, we studied if hypertrophic cardiomyopathy-linked mutation (TnT R94H )-induced effects on contractile function would be differently modulated by sarcomere length (SL). Recombinant wild-type TnT (TnT WT ) and the guinea pig analog of the human R94H mutation (TnT R95H ) were reconstituted into detergent-skinned cardiac muscle fibers from guinea pigs. Steady-state and dynamic contractile measurements were made at short and long SLs (1.9 and 2.3 µm, respectively). Our results demonstrated that TnT R95H increased pCa 50 (-log of free Ca 2+ concentration) to a greater extent at short SL; TnT R95H increased pCa 50 by 0.11 pCa units at short SL and 0.07 pCa units at long SL. The increase in pCa 50 associated with an increase in SL from 1.9 to 2.3 µm (ΔpCa 50 ) was attenuated nearly twofold in TnT R95H fibers; ΔpCa 50 was 0.09 pCa units for TnT WT fibers but only 0.05 pCa units for TnT R95H fibers. The SL dependency of rate constants of cross-bridge distortion dynamics and tension redevelopment was also blunted by TnT R95H Collectively, our observations on the SL dependency of pCa 50 and rate constants of cross-bridge distortion dynamics and tension redevelopment suggest that mechanisms underlying the length-dependent activation cardiac myofilaments are attenuated by TnT R95H NEW & NOTEWORTHY Mutant cardiac troponin T (TnT R95H ) differently affects myofilament Ca 2+ sensitivity at short and long sarcomere length, indicating that mechanisms underlying length-dependent activation are altered by TnT R95H TnT R95H enhances myofilament Ca 2+ sensitivity to a greater extent at short sarcomere length, thus attenuating the length-dependent increase in myofilament Ca 2+ sensitivity. Copyright © 2017 the American Physiological Society.
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68. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF COLUMBUS ca. 1875 ...
68. MISSISSIPPI, LOWNDES CO. COLUMBUS MAP OF COLUMBUS ca. 1875 BIRD'S EYE VIEW OF COLUMBUS MISSISSIPPI by Camille Drie ca. 1875. Copy of snapshot in Lowndes Co. Public Library, Columbus, Ms. Snow status in early 1870s: includes M&O RR bridge, but no highway bridge. Sarcone Photography, Columbus, Ms., Sept 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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75 FR 16006 - Drawbridge Operation Regulation; Sacramento River, Sacramento, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2010-03-31
... Operation Regulation; Sacramento River, Sacramento, CA AGENCY: Coast Guard, DHS. ACTION: Notice of temporary..., mile 59.4, at Sacramento, CA. The deviation is necessary to allow the bridge owner to make bridge... Sacramento River, at Sacramento, CA. The I Street Drawbridge navigation span provides 109 feet vertical...
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78 FR 42452 - Drawbridge Operation Regulation; Sacramento River, Sacramento, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2013-07-16
... Operation Regulation; Sacramento River, Sacramento, CA AGENCY: Coast Guard, DHS. ACTION: Notice of deviation... Sacramento, CA. The deviation is necessary to allow the bridge owner to make bridge repairs. This deviation... Sacramento, CA. The drawbridge navigation span provides 109 feet vertical clearance above Mean High Water in...
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Federal Register 2010, 2011, 2012, 2013, 2014
2012-08-23
...-AA00 Safety Zone: Bay Bridge Load Transfer Safety Zone, San Francisco Bay, San Francisco, CA AGENCY... the Bay Bridge Load Transfer Safety Zone from August 1, 2012 through October 31, 2012. This safety... Bay Bridge from the temporary suspension arrangement to the permanent suspension arrangement, the...
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Alaimo, Alysha A; Koumousi, Evangelia S; Cunha-Silva, Luís; McCormick, Laura J; Teat, Simon J; Psycharis, Vassilis; Raptopoulou, Catherine P; Mukherjee, Shreya; Li, Chaoran; Gupta, Sayak Das; Escuer, Albert; Christou, George; Stamatatos, Theocharis C
2017-09-05
One-pot reactions between the [Mn 3 O(O 2 CPh) 6 (py) x ] +/0 triangular precursors and either CaBr 2 ·xH 2 O or CaCl 2 ·6H 2 O, in the presence of salicylhydroxamic acid (shaH 2 ), have afforded the heterometallic complexes [Mn III 4 Ca 2 (O 2 CPh) 4 (shi) 4 (H 2 O) 3 (Me 2 CO)] (1) and (pyH)[Mn II 2 Mn III 4 Ca 2 Cl 2 (O 2 CPh) 7 (shi) 4 (py) 4 ] (2), respectively, in good yields. Further reactions but using a more flexible synthetic scheme comprising the Mn(NO 3 ) 2 ·4H 2 O/Ca(NO 3 ) 2 ·4H 2 O and Mn(O 2 CPh) 2 ·2H 2 O/Ca(ClO 4 ) 2 ·4H 2 O "metal blends" and shaH 2 , in the presence of external base NEt 3 , led to the new complexes (NHEt 3 ) 2 [Mn III 4 Mn IV 4 Ca(OEt) 2 (shi) 10 (EtOH) 2 ] (3) and (NHEt 3 ) 4 [Mn III 8 Ca 2 (CO 3 ) 4 (shi) 8 ] (4), respectively. In all reported compounds, the anion of the tetradentate (N,O,O,O)-chelating/bridging ligand salicylhydroxime (shi 3- ), resulting from the in situ metal-ion-assisted amide-iminol tautomerism of shaH 2 , was found to bridge both Mn and Ca atoms. Complexes 1-4 exhibit a variety of different structures, metal stoichiometries, and Mn oxidation-state descriptions; 1 possesses an overall octahedral metal arrangement, 2 can be described as a Mn 4 Ca 2 octahedron bound to an additional Mn 2 unit, 3 consists of a Mn 8 "ring" surrounding a Ca II atom, and 4 adopts a rectangular cuboidal motif of eight Mn atoms accommodating two Ca II atoms. Solid-state direct-current magnetic susceptibility studies revealed the presence of predominant antiferromagnetic exchange interactions between the Mn centers, leading to S = 0 spin ground-state values for all complexes. From a bioinorganic chemistry perspective, the reported compounds may demonstrate some relevance to both high-valent scheme (3) and lower-oxidation-level species (1, 2, and 4) of the catalytic cycle of the oxygen-evolving complex.
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PLANT AND PIER #2 EXCAVATION. View is to the northeast, ...
PLANT AND PIER #2 EXCAVATION. View is to the northeast, looking from Humboldt County side of river toward Trinity County side - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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MOVING SHAFT FORMS FROM PIER #2 TO PIER #1. View ...
MOVING SHAFT FORMS FROM PIER #2 TO PIER #1. View is to the northeast, with shaft forms being moved by highline - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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34. ALTERNATE DESIGN USING BATTERED AND UNSHEATHED LIFT TOWERS, WITH ...
34. ALTERNATE DESIGN USING BATTERED AND UNSHEATHED LIFT TOWERS, WITH DEEPENED TRUSS ON LIFT SPAN. Pen-and-ink drawing by project architect Alfred Eichler, 1934. - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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POURING FOOTING OF PIER #1. View is to the southwest, ...
POURING FOOTING OF PIER #1. View is to the southwest, looking from Pier 2 in Trinity County toward Pier 1 in Humboldt County - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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22. UPSTREAM VIEW OF THE OUTLET CONTROL STRUCTURE AND THE ...
22. UPSTREAM VIEW OF THE OUTLET CONTROL STRUCTURE AND THE PIER FOR THE SERVICE BRIDGE.... Volume XVIII, No. 12, January 29, 1940. - Prado Dam, Outlet Works, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA
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View of substructure of Sixth Street Bridge overcrossing of Los ...
View of substructure of Sixth Street Bridge overcrossing of Los Angeles River. Looking west. Note dark hole at lower with is access ramp to river channel seen in HAER CA-176-56 - Sixth Street Bridge, Spanning 101 Freeway at Sixth Street, Los Angeles, Los Angeles County, CA
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Slowed Relaxation in Fatigued Skeletal Muscle Fibers of Xenopus and Mouse
Westerblad, Håkan; Lännergren, Jan; Allen, David G.
1997-01-01
Slowing of relaxation is an important characteristic of skeletal muscle fatigue. The aim of the present study was to quantify the relative contribution of altered Ca2+ handling (calcium component) and factors down-stream to Ca2+ (cross-bridge component) to the slowing of relaxation in fatigued fibers of Xenopus and mouse. Two types of Xenopus fibers were used: easily fatigued, type 1 fibers and fatigue resistant, type 2 fibers. In these Xenopus fibers the free myoplasmic [Ca2+] ([Ca2+]i) was measured with indo-1, and the relaxation of Ca2+-derived force, constructed from tetanic [Ca2+]i records and in vivo [Ca2+]i-force curves, was analyzed. An alternative method was used in both Xenopus and mouse fibers: fibers were rapidly shortened during the initial phase of relaxation, and the time to the peak of force redevelopment was measured. These two methods gave similar results and showed proportional slowing of the calcium and cross-bridge components of relaxation in both fatigued type 1 and type 2 Xenopus fibers, whereas only the cross-bridge component was slowed in fatigued mouse fibers. Ca2+ removal from the myoplasm during relaxation was markedly less effective in Xenopus fibers as compared to mouse fibers. Fatigued Xenopus fibers displayed a reduced rate of sarcoplasmic reticulum Ca2+ uptake and increased sarcoplasmic reticulum Ca2+ leak. Some fibers were stretched at various times during relaxation. The resistance to these stretches was increased during fatigue, especially in Xenopus fibers, which indicates that longitudinal movements during relaxation had become less pronounced and this might contribute to the increased cross-bridge component of relaxation in fatigue. In conclusion, slowing of relaxation in fatigued Xenopus fibers is caused by impaired Ca2+ handling and altered cross-bridge kinetics, whereas the slowing in mouse fibers is only due to altered cross-bridge kinetics. PMID:9089444
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Shiah, Amy A.; Gandra, Paulo G.; Hogan, Michael C.
2013-01-01
The energy cost of contractions in skeletal muscle involves activation of both actomyosin and sarcoplasmic reticulum (SR) Ca2+-pump (SERCA) ATPases, which together determine the overall ATP demand. During repetitive contractions leading to fatigue, the relaxation rate and Ca2+ pumping become slowed, possibly because of intracellular metabolite accumulation. The role of the energy cost of cross-bridge cycling during contractile activity on Ca2+-pumping properties has not been investigated. Therefore, we inhibited cross-bridge cycling by incubating isolated Xenopus single fibers with N-benzyl-p-toluene sulfonamide (BTS) to study the mechanisms by which SR Ca2+ pumping is impaired during fatiguing contractions. Fibers were stimulated in the absence (control) and presence of BTS and cytosolic calcium ([Ca2+]c) transients or intracellular pH (pHi) changes were measured. BTS treatment allowed normal [Ca2+]c transients during stimulation without cross-bridge activation. At the time point that tension was reduced to 50% in the control condition, the fall in the peak [Ca2+]c and the increase in basal [Ca2+]c did not occur with BTS incubation. The progressively slower Ca2+ pumping rate and the fall in pHi during repetitive contractions were reduced during BTS conditions. However, when mitochondrial ATP supply was blocked during contractions with BTS present (BTS + cyanide), there was no further slowing in SR Ca2+ pumping during contractions compared with the BTS-alone condition. Furthermore, the fall in pHi was significantly less during the BTS + cyanide condition than in the control conditions. These results demonstrate that factors related to the energetic cost of cross-bridge cycling, possibly the accumulation of metabolites, inhibit the Ca2+ pumping rate during fatiguing contractions. PMID:23678027
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Modeling Violent Non-State Actors: A Summary of Concepts and Methods
2004-11-01
charts, leadership, rules, formal communications and process efficiency to name a few. While a useful aspect of organizational diagnosis , this...and Arie Shirom, Organizational Diagnosis and Assessment: Bridging Theory and Practice (Thousand Oaks, CA: Sage Publications, 1999), 44. 9 Katz and
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Turbulent Arena: Global Effects Against Non-State Adversaries
2005-06-01
process efficiency to name a few. While a useful aspect of organizational diagnosis , this closed system approach neglects the simple reality that an... Organizational Diagnosis and Assessment: Bridging Theory and Practice (Thousand Oaks, CA: Sage Publications, 1999), 44. 13 Katz and Kahn, 262. 14 Daft
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20. VIEW TO SOUTHWEST. DOLPHIN. (Photographed from boat) NOTE CUTWATER ...
20. VIEW TO SOUTHWEST. DOLPHIN. (Photographed from boat) NOTE CUTWATER ON UPSTREAM SIDE OF DOLPHIN, AND THAT DOLPHIN IS OCTAGONAL AS OPPOSED TO CIRCULAR DESIGN OF CENTER PIER. - Gianella Bridge, Spanning Sacramento River at State Highway 32, Hamilton City, Glenn County, CA
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35. ALTERNATE DESIGN USING THROUGH ARCH SPANS, WITH ARCH REPEATED ...
35. ALTERNATE DESIGN USING THROUGH ARCH SPANS, WITH ARCH REPEATED BETWEEN TOWER LEGS, AND ASHLAR MASONRY WALLS AND PYLONS Pen-and-ink drawing by project architect Alfred Eichler, 1934. - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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Impact. The southwest end is down, as the buckled middle ...
Impact. The southwest end is down, as the buckled middle section splashes into the river. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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24. VIEW, LOOKING NORTHEAST, SHOWING MAIN TRANSMISSION IN LEFT FOREGROUND, ...
24. VIEW, LOOKING NORTHEAST, SHOWING MAIN TRANSMISSION IN LEFT FOREGROUND, GASOLINE-POWERED WAUKESHA AUXILIARY DRIVE MOTOR AT CENTER, AND ONE OF TWO MAIN ELECTRIC DRIVE MOTORS AT LEFT CENTER - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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39. PENCIL SKETCH OF SELECTED DESIGN, DEPICTING OCEANGOING VESSEL (WHICH ...
39. PENCIL SKETCH OF SELECTED DESIGN, DEPICTING OCEAN-GOING VESSEL (WHICH WERE STILL NAVIGATING SACRAMENTO RIVER IN 1930s) APPROACHING LIFT SPAN Drawn by project architect Alfred Eichler, 1935 - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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AT&SF RAILROAD BRIDGE ACROSS ARROYO SECO CHANNEL AND PARKWAY. SEEN ...
AT&SF RAILROAD BRIDGE ACROSS ARROYO SECO CHANNEL AND PARKWAY. SEEN FROM DEBS PARK IN SAME CAMERA LOCATION AS CA-265-13. NOTE FREEWAY 134 AT DISTANT RIGHT REAR. LOOKING 318°NW - Atchison, Topeka & Santa Fe Railroad Bridge, Spanning Arroyo Seco Parkway at parkway milepost 29.03, Los Angeles, Los Angeles County, CA
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Smooth muscle contraction: mechanochemical formulation for homogeneous finite strains.
Stålhand, J; Klarbring, A; Holzapfel, G A
2008-01-01
Chemical kinetics of smooth muscle contraction affect mechanical properties of organs that function under finite strains. In an effort to gain further insight into organ physiology, we formulate a mechanochemical finite strain model by considering the interaction between mechanical and biochemical components of cell function during activation. We propose a new constitutive framework and use a mechanochemical device that consists of two parallel elements: (i) spring for the cell stiffness; (ii) contractile element for the sarcomere. We use a multiplicative decomposition of cell elongation into filament contraction and cross-bridge deformation, and suggest that the free energy be a function of stretches, four variables (free unphosphorylated myosin, phosphorylated cross-bridges, phosphorylated and dephosphorylated cross-bridges attached to actin), chemical state variable driven by Ca2+-concentration, and temperature. The derived constitutive laws are thermodynamically consistent. Assuming isothermal conditions, we specialize the mechanical phase such that we recover the linear model of Yang et al. [2003a. The myogenic response in isolated rat cerebrovascular arteries: smooth muscle cell. Med. Eng. Phys. 25, 691-709]. The chemical phase is also specialized so that the linearized chemical evolution law leads to the four-state model of Hai and Murphy [1988. Cross-bridge phosphorylation and regulation of latch state in smooth muscle. Am. J. Physiol. 254, C99-C106]. One numerical example shows typical mechanochemical effects and the efficiency of the proposed approach. We discuss related parameter identification, and illustrate the dependence of muscle contraction (Ca2+-concentration) on active stress and related stretch. Mechanochemical models of this kind serve the mathematical basis for analyzing coupled processes such as the dependency of tissue properties on the chemical kinetics of smooth muscle.
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78 FR 35758 - Drawbridge Operation Regulation; Bishop Cut, Near Stockton, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2013-06-14
... Operation Regulation; Bishop Cut, Near Stockton, CA AGENCY: Coast Guard, DHS. ACTION: Notice of deviation... operating regulation that governs the San Joaquin County Highway Bridge across Bishop Cut, mile 1.0, near... Highway Bridge, mile 1.0, over Bishop Cut, near Stockton, CA. The drawbridge navigation span provides a...
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20. VIEW, LOOKING SOUTH, SHOWING ONE OF TWO MAIN ELECTRIC ...
20. VIEW, LOOKING SOUTH, SHOWING ONE OF TWO MAIN ELECTRIC MOTORS IN FOREGROUND WITH REDUCTION GEAR HOUSING AT RIGHT, GASOLINE- POWERED WAUKESHA AUXILIARY DRIVE MOTOR AT MIDDLE, AND GASOLINE- POWERED EMERGENCY GENERATOR IN BACKGROUND - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA
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3. DOWNSTREAM AERIAL VIEW OF THE DIVERSION CHANNEL AND CONTROL ...
3. DOWNSTREAM AERIAL VIEW OF THE DIVERSION CHANNEL AND CONTROL WORKS. THE OUTLET CONTROL TOWER AND THE PIER FOR THE SERVICE BRIDGE ARE SHOWN COMPLETED.... Volume XVIII, No. 11, January 18, 1940. - Prado Dam, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA
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Nogueira, Leonardo; Shiah, Amy A; Gandra, Paulo G; Hogan, Michael C
2013-07-15
The energy cost of contractions in skeletal muscle involves activation of both actomyosin and sarcoplasmic reticulum (SR) Ca²⁺-pump (SERCA) ATPases, which together determine the overall ATP demand. During repetitive contractions leading to fatigue, the relaxation rate and Ca²⁺ pumping become slowed, possibly because of intracellular metabolite accumulation. The role of the energy cost of cross-bridge cycling during contractile activity on Ca²⁺-pumping properties has not been investigated. Therefore, we inhibited cross-bridge cycling by incubating isolated Xenopus single fibers with N-benzyl-p-toluene sulfonamide (BTS) to study the mechanisms by which SR Ca²⁺ pumping is impaired during fatiguing contractions. Fibers were stimulated in the absence (control) and presence of BTS and cytosolic calcium ([Ca²⁺]c) transients or intracellular pH (pHi) changes were measured. BTS treatment allowed normal [Ca²⁺]c transients during stimulation without cross-bridge activation. At the time point that tension was reduced to 50% in the control condition, the fall in the peak [Ca²⁺]c and the increase in basal [Ca²⁺]c did not occur with BTS incubation. The progressively slower Ca²⁺ pumping rate and the fall in pHi during repetitive contractions were reduced during BTS conditions. However, when mitochondrial ATP supply was blocked during contractions with BTS present (BTS + cyanide), there was no further slowing in SR Ca²⁺ pumping during contractions compared with the BTS-alone condition. Furthermore, the fall in pHi was significantly less during the BTS + cyanide condition than in the control conditions. These results demonstrate that factors related to the energetic cost of cross-bridge cycling, possibly the accumulation of metabolites, inhibit the Ca²⁺ pumping rate during fatiguing contractions.
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Baxley, Tamatha; Johnson, Dylan; Pinto, Jose R; Chalovich, Joseph M
2017-06-13
Striated muscle contraction is regulated by the actin-associated proteins tropomyosin and troponin. The extent of activation of myosin ATPase activity is lowest in the absence of both Ca 2+ and activating cross-bridges (i.e., S1-ADP or rigor S1). Binding of activating species of myosin to actin at a saturating Ca 2+ concentration stabilizes the most active state (M state) of the actin-tropomyosin-troponin complex (regulated actin). Ca 2+ binding alone produces partial stabilization of the active state. The extent of stabilization at a saturating Ca 2+ concentration depends on the isoform of the troponin subunits, the phosphorylation state of troponin, and, in the case of cardiac muscle, the presence of hypertrophic cardiomyopathy-producing mutants of troponin T and troponin I. Cardiac dysfunction is also associated with mutations of troponin C (TnC). Troponin C mutants A8V, C84Y, and D145E increase the Ca 2+ sensitivity of ATPase activity. We show that these mutants change the distribution of regulated actin states. The A8V and C84Y TnC mutants decreased the inactive B state distribution slightly at low Ca 2+ concentrations, but the D145E mutants had no effect on that state. All TnC mutants increased the level of the active M state compared to that of the wild type, at a saturating Ca 2+ concentration. Troponin complexes that contained two mutations that stabilize the active M state, A8V TnC and Δ14 TnT, appeared to be completely in the active state in the presence of only Ca 2+ . Because Ca 2+ gives full activation, in this situation, troponin must be capable of positioning tropomyosin in the active M state without the need for rigor myosin binding.
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43. Photocopy of photograph, photographer unknown, ca January 1929 (original ...
43. Photocopy of photograph, photographer unknown, ca January 1929 (original print located at Arizona Department of Transportation, Phoenix AZ). COMPLETED BRIDGE. - Navajo Bridge, Spanning Colorado River at U.S. Highway 89 Alternate, Page, Coconino County, AZ
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Results of celiac trunk stenting during fenestrated or branched aortic endografting.
Wattez, Hélène; Martin-Gonzalez, Teresa; Lopez, Benjamin; Spear, Rafaëlle; Clough, Rachel E; Hertault, Adrien; Sobocinski, Jonathan; Haulon, Stéphan
2016-12-01
Endovascular repair of aortic aneurysms involving the visceral segment of the aorta often requires placement of a covered bridging stent in the celiac axis (CA). The median arcuate ligament (MAL) is a fibrous arch that unites the diaphragmatic crura on either side of the aortic hiatus. The ligament may compress and distort the celiac artery and result in difficult cannulation, or stenosis and occlusion of the vessel. This study evaluated the influence of the MAL compression on the technical success and the patency of the celiac artery after branched and fenestrated endovascular aortic repair. We retrospectively analyzed a cohort of consecutive patients treated electively for complex aneurysms with branched and fenestrated endovascular aortic repair between January 2007 and April 2014. All data were collected prospectively. Analysis of preoperative computed tomography angiography on a three-dimensional workstation determined the presence of MAL compression. Patency of the CA bridging stent was assessed during follow-up by computed tomography angiography and duplex ultrasound evaluation. Statistical analysis was performed to compare the outcomes of patients with MAL (MAL+) and without MAL (MAL-) compression. Of 315 patients treated for aortic disease involving the visceral segment during the study period, 113 had endografts designed with a branch (n = 57) or fenestration (n = 56) for the CA. In 45 patients (39.8%), asymptomatic compression of the CA by the MAL was depicted (MAL+). Complex endovascular techniques were required in this group to access the CA in 16 (14.2%) patients (vs none in the MAL- group; P = .003), which lead to a failed bridging stent implantation in seven patients (6.2%). Increased operative time and dose area product were observed in the MAL+ group, but this did not reach statistical significance. In the MAL+ group, no thrombosis of the CA bridging stents were observed during follow-up; an external compression of the CA bridging stent was depicted in six patients but without hemodynamic effect on duplex ultrasound imaging. In the MAL- group, one CA bridging stent occlusion occurred owing to an embolus from a cardiac source. MAL compression is associated with good celiac trunk bridging stent patency during follow-up, but with a higher rate of technical difficulties and failed bridging stent implantation during the procedure. Copyright © 2016 Society for Vascular Surgery. Published by Elsevier Inc. All rights reserved.
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NASA Astrophysics Data System (ADS)
Yeom, Bongjun; Char, Kookheon
2016-06-01
Laminated nanostructures in nacre have been adopted as models in the fabrication of strong, tough synthetic nanocomposites. However, the utilization of CaCO3 biominerals in these composites is limited by the complexity of the synthesis method for nanosized biominerals. In this study, we use the enzymatic reaction of urease to generate a nanoscale CaCO3 thin film to prepare CaCO3/polymer hybrid nanolaminates. Additional layers of CaCO3 thin film are consecutively grown over the base CaCO3 layer with the intercalation of organic layers. The morphology and crystallinity of the added CaCO3 layers depend strongly on the thickness of the organic layer coated on the underlying CaCO3 layer. When the organic layer is less than 20 nm thick, the amorphous CaCO3 layer is spontaneously transformed into crystalline calcite layer during the growth process. We also observe crystalline continuity between adjacent CaCO3 layers through interconnecting mineral bridges. The formation of these mineral bridges is crucial to the epitaxial growth of CaCO3 layers, similar to the formation of natural nacre.
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Liba, Benjamin D; Kim, Eunkyoung; Martin, Alexandra N; Liu, Yi; Bentley, William E; Payne, Gregory F
2013-03-01
Exciting opportunities in bioelectronics will be facilitated by materials that can bridge the chemical logic of biology and the digital logic of electronics. Here we report the fabrication of a dual functional hydrogel film that can harvest electrons from its chemical environment and store these electrons by switching the film's redox-state. The hydrogel scaffold was formed by the anodic deposition of the aminopolysaccharide chitosan. Electron-harvesting function was conferred by co-depositing the enzyme glucose dehydrogenase (GDH) with chitosan. GDH catalyzes the transfer of electrons from glucose to the soluble redox-shuttle NADP(+). Electron-storage function was conferred by the redox-active food phenolic chlorogenic acid (CA) that was enzymatically grafted to the chitosan scaffold using tyrosinase. The grafted CA undergoes redox-cycling reactions with NADPH resulting in the net transfer of electrons to the film where they are stored in the reduced state of CA. The individual and dual functionalities of these films were demonstrated experimentally. There are three general conclusions from this proof-of-concept study. First, enzymatically-grafted catecholic moieties confer redox-capacitor function to the chitosan scaffold. Second, biological materials (i.e. chitosan and CA) and mechanisms (i.e. tyrosinase-mediated grafting) allow the reagentless fabrication of functional films that should be environmentally-friendly, safe and potentially even edible. Finally, the film's ability to mediate the transfer of electrons from a biological metabolite to an electrode suggests an approach to bridge the chemical logic of biology with the digital logic of electronics.
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9. Photocopy of photograph (original print at Riverside Library, Local ...
9. Photocopy of photograph (original print at Riverside Library, Local History Collection), photographer unknown, ca. 1903-04. VIEW OF WORKERS AND BRIDGE UNDER CONSTRUCTION - Union Pacific Railroad Bridge, Spanning Santa Anna River, west of Riverside, Riverside, Riverside County, CA
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Spray and dust hang in the air, as the demolition ...
Spray and dust hang in the air, as the demolition is completed. Only the southeast pier remains standing at right. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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47. N.R.P., photographer January 14, 1938 MENDOCINO COUNTY, SECTION, HIGHWAY ...
47. N.R.P., photographer January 14, 1938 MENDOCINO COUNTY, SECTION, HIGHWAY 1. 1-MEN-1-J #144, ROCK CREEK BRIDGE. SO., 1-14-38, N.R.P. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA
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18. Interior view, 'Inside Key Route Inspection Bldg.', view to ...
18. Interior view, 'Inside Key Route Inspection Bldg.', view to east, August 16, 1939. Note inspection pits and work areas beneath tracks. The Key Route and Interurban Electric Railway Bridge Yard Shop buildings were identical, and this view provides an in-service look at the well-lit interior. While Key Route articulated cars were quite different in design from the Interurban Electric Railway cars, the maintenance requirements were quite similar. The Key Route Bridge Yard Shop building was demolished in the 1970s. Its last use was storage of much of the historic railway equipment now on display at the California State Railroad Museum in Sacramento. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA
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Fajmut, Ales; Dobovisek, Andrej; Brumen, Milan
2005-01-01
In this paper the 4-state latch bridge model proposed by Rembold and Murphy is expanded; first by incorporation of the analytical expression of Ca2+ dependent MLCK activation from the work of Kato et al. and second, by inclusion of the myosin dephosphorylation based on the Michaelis-Menten kinetics. The analysis of the proposed model and the comparison with the original model results as well as with the experimental data is presented. The model is able to predict the steady-state isometric stress and the myosin phosphorylation in dependence on steady cytosolic [Ca2+] as well as the temporal evolution of the system in dependence on the input Ca2+ signal in the form of biphasic transient, whereby our model results are in several aspects in better agreement with experimental observations.
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Reverse actin sliding triggers strong myosin binding that moves tropomyosin
DOE Office of Scientific and Technical Information (OSTI.GOV)
Bekyarova, T.I.; Reedy, M.C.; Baumann, B.A.J.
2008-09-03
Actin/myosin interactions in vertebrate striated muscles are believed to be regulated by the 'steric blocking' mechanism whereby the binding of calcium to the troponin complex allows tropomyosin (TM) to change position on actin, acting as a molecular switch that blocks or allows myosin heads to interact with actin. Movement of TM during activation is initiated by interaction of Ca{sup 2+} with troponin, then completed by further displacement by strong binding cross-bridges. We report x-ray evidence that TM in insect flight muscle (IFM) moves in a manner consistent with the steric blocking mechanism. We find that both isometric contraction, at highmore » [Ca{sup 2+}], and stretch activation, at lower [Ca{sup 2+}], develop similarly high x-ray intensities on the IFM fourth actin layer line because of TM movement, coinciding with x-ray signals of strong-binding cross-bridge attachment to helically favored 'actin target zones.' Vanadate (Vi), a phosphate analog that inhibits active cross-bridge cycling, abolishes all active force in IFM, allowing high [Ca{sup 2+}] to elicit initial TM movement without cross-bridge attachment or other changes from relaxed structure. However, when stretched in high [Ca{sup 2+}], Vi-'paralyzed' fibers produce force substantially above passive response at pCa {approx} 9, concurrent with full conversion from resting to active x-ray pattern, including x-ray signals of cross-bridge strong-binding and TM movement. This argues that myosin heads can be recruited as strong-binding 'brakes' by backward-sliding, calcium-activated thin filaments, and are as effective in moving TM as actively force-producing cross-bridges. Such recruitment of myosin as brakes may be the major mechanism resisting extension during lengthening contractions.« less
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jacking Item 22: Bridge removal (column) Item 23: Bridge removal (portion) Item 24: Approach slab removal for: Search Menu Log in Register PEER Center - 325 Davis Hall, University of California, Berkeley, CA
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Fropofol decreases force development in cardiac muscle.
Ren, Xianfeng; Schmidt, William; Huang, Yiyuan; Lu, Haisong; Liu, Wenjie; Bu, Weiming; Eckenhoff, Roderic; Cammarato, Anthony; Gao, Wei Dong
2018-03-09
Supranormal contractile properties are frequently associated with cardiac diseases. Anesthetic agents, including propofol, can depress myocardial contraction. We tested the hypothesis that fropofol, a propofol derivative, reduces force development in cardiac muscles via inhibition of cross-bridge cycling and may therefore have therapeutic potential. Force and intracellular Ca 2+ ([Ca 2+ ] i ) transients of rat trabecular muscles were determined. Myofilament ATPase, actin-activated myosin ATPase, and velocity of actin filaments propelled by myosin were also measured. Fropofol dose dependently decreased force without altering [Ca 2+ ] i in normal and pressure-induced hypertrophied-hypercontractile muscles. Similarly, fropofol depressed maximum Ca 2+ -activated force ( F max ) and increased the [Ca 2+ ] i required for 50% activation at steady-state (Ca 50 ) without affecting the Hill coefficient in both intact and skinned cardiac fibers. The drug also depressed cardiac myofibrillar and actin-activated myosin ATPase activity. In vitro actin sliding velocity was significantly reduced when fropofol was introduced during rigor binding of cross-bridges. The data suggest that the depressing effects of fropofol on cardiac contractility are likely to be related to direct targeting of actomyosin interactions. From a clinical standpoint, these findings are particularly significant, given that fropofol is a nonanesthetic small molecule that decreases myocardial contractility specifically and thus may be useful in the treatment of hypercontractile cardiac disorders.-Ren, X., Schmidt, W., Huang, Y., Lu, H., Liu, W., Bu, W., Eckenhoff, R., Cammarato, A., Gao, W. D. Fropofol decreases force development in cardiac muscle.
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Synergistic role of ADP and Ca2+ in diastolic myocardial stiffness
Sequeira, Vasco; Najafi, Aref; McConnell, Mark; Fowler, Ewan D; Bollen, Ilse A E; Wüst, Rob C I; dos Remedios, Cris; Helmes, Michiel; White, Ed; Stienen, Ger J M; Tardiff, Jil; Kuster, Diederik W D; van der Velden, Jolanda
2015-01-01
Abstract Heart failure (HF) with diastolic dysfunction has been attributed to increased myocardial stiffness that limits proper filling of the ventricle. Altered cross-bridge interaction may significantly contribute to high diastolic stiffness, but this has not been shown thus far. Cross-bridge interactions are dependent on cytosolic [Ca2+] and the regeneration of ATP from ADP. Depletion of myocardial energy reserve is a hallmark of HF leading to ADP accumulation and disturbed Ca2+ handling. Here, we investigated if ADP elevation in concert with increased diastolic [Ca2+] promotes diastolic cross-bridge formation and force generation and thereby increases diastolic stiffness. ADP dose-dependently increased force production in the absence of Ca2+ in membrane-permeabilized cardiomyocytes from human hearts. Moreover, physiological levels of ADP increased actomyosin force generation in the presence of Ca2+ both in human and rat membrane-permeabilized cardiomyocytes. Diastolic stress measured at physiological lattice spacing and 37°C in the presence of pathological levels of ADP and diastolic [Ca2+] revealed a 76 ± 1% contribution of cross-bridge interaction to total diastolic stress in rat membrane-permeabilized cardiomyocytes. Inhibition of creatine kinase (CK), which increases cytosolic ADP, in enzyme-isolated intact rat cardiomyocytes impaired diastolic re-lengthening associated with diastolic Ca2+ overload. In isolated Langendorff-perfused rat hearts, CK inhibition increased ventricular stiffness only in the presence of diastolic [Ca2+]. We propose that elevations of intracellular ADP in specific types of cardiac disease, including those where myocardial energy reserve is limited, contribute to diastolic dysfunction by recruiting cross-bridges, even at low Ca2+, and thereby increase myocardial stiffness. Key points Diastolic dysfunction in heart failure patients is evident from stiffening of the passive properties of the ventricular wall. Increased actomyosin interactions may significantly limit diastolic capacity, however, direct evidence is absent. From experiments at the cellular and whole organ level, in humans and rats, we show that actomyosin-related force development contributes significantly to high diastolic stiffness in environments where high ADP and increased diastolic [Ca2+] are present, such as the failing myocardium. Our basal study provides a mechanical mechanism which may partly underlie diastolic dysfunction. PMID:26096258
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Using optical tweezers to relate the chemical and mechanical cross-bridge cycles.
Steffen, Walter; Sleep, John
2004-12-29
In most current models of muscle contraction there are two translational steps, the working stroke, whereby an attached myosin cross-bridge moves relative to the actin filament, and the repriming step, in which the cross-bridge returns to its original orientation. The development of single molecule methods has allowed a more detailed investigation of the relationship of these mechanical steps to the underlying biochemistry. In the normal adenosine triphosphate cycle, myosin.adenosine diphosphate.phosphate (M.ADP.Pi) binds to actin and moves it by ca. 5 nm on average before the formation of the end product, the rigor actomyosin state. All the other product-like intermediate states tested were found to give no net movement indicating that M.ADP.Pi alone binds in a pre-force state. Myosin states with bound, unhydrolysed nucleoside triphosphates also give no net movement, indicating that these must also bind in a post-force conformation and that the repriming, post- to pre-transition during the forward cycle must take place while the myosin is dissociated from actin. These observations fit in well with the structural model in which the working stroke is aligned to the opening of the switch 2 element of the ATPase site.
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8. West side of north end of bridge resting on ...
8. West side of north end of bridge resting on approach abutment. Oblique detail view northeast (from below roadbed level, beside bridge). 150 mm lens. - Gault Bridge, Spanning Deer Creek at South Pine Street, Nevada City, Nevada County, CA
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Suspension Bridge Structural Systems: Cable Suspension & Anchorage; Warren Stiffening ...
Suspension Bridge Structural Systems: Cable Suspension & Anchorage; Warren Stiffening Truss; Upper & Lower Decks; Assembled System - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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64. Photocopy of photograph (original print in the Minnesota Historical ...
64. Photocopy of photograph (original print in the Minnesota Historical Society, St. Paul Minnesota, ca. 1891. #35805. Photographer: Haynes and Bros., St. Paul.) VIEW TO SOUTH, SHOWING FOUR PERCENT GRADE OF HIGH BRIDGE, ca. 1981 - Smith Avenue High Bridge, Smith Avenue between Cherokee Avenue & Cliff Street, Saint Paul, Ramsey County, MN
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78 FR 34892 - Drawbridge Operation Regulation; Middle River, Near Stockton, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2013-06-11
..., CA. The deviation is to allow San Joaquin County Public Works Department to perform structural maintenance work to the bridge. This deviation allows the bridge to remain in the closed-to-navigation... West Building, 1200 New Jersey Avenue SE., Washington, DC 20590, between 9 a.m. and 5 p.m., Monday...
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18. View to southwest. Detail, bearing shoe, upstream side of ...
18. View to southwest. Detail, bearing shoe, upstream side of east pier. Copy negative made from 35mm color transparency made with with 135mm lens by John Snyder, due to lack of sufficiently long lens for 4x5 camera. - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA
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19. DETAIL VIEW SHOWING POINT OF CONNECTION OF SWAY BRACING ...
19. DETAIL VIEW SHOWING POINT OF CONNECTION OF SWAY BRACING TO UPSTREAM ARCH RIB AT SPRING POINT FROM SOUTH ABUTMENT, LOOKING EAST-NORTHEAST. NOTE CRACK VISIBLE NEAR TOP OF SWAY BRACING AT LEFT CENTER, PROBABLY DUE TO RUST JACKING OF REINFORCING STEEL - Chili Bar Bridge, Spanning South Fork of American River at State Highway 193, Placerville, El Dorado County, CA
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20. General east to west elevated view of Moody Bridge ...
20. General east to west elevated view of Moody Bridge placing it within and in relation to the surrounding rural environment. - Moody Bridge, Spanning South Fork Eel River, Garberville, Humboldt County, CA
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22. EXTERIOR DETAIL VIEW OF SOUTHEAST CORNER OF BRIDGE, 1932 ...
22. EXTERIOR DETAIL VIEW OF SOUTHEAST CORNER OF BRIDGE, 1932 SECTION, SOUTH BANK, LOOKING NORTHEAST TO SOUTHWEST - Rowdy Creek Bridge, Spanning Rowdy Creek at Fred Haight Drive, Smith River, Del Norte County, CA
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7. LASSEN PARK ROAD BRIDGE AT SULFUR WORKS. NOTE ROAD ...
7. LASSEN PARK ROAD BRIDGE AT SULFUR WORKS. NOTE ROAD TRAVERSING DISTANT RIDGE BEYOND BRIDGE. SEEN FROM WEST OF HIGHWAY FROM OLD HIGHWAY LOOP. LOOKING E. - Lassen Park Road, Mineral, Tehama County, CA
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13. Photocopy of a photographca. 1896showing wooden arch bridge over ...
13. Photocopy of a photograph--ca. 1896--showing wooden arch bridge over the North Fork of the San Joaquin River northeast of Fresno, CA. This structure was designed by Eastwood as part of the San Joaquin Electric Company's hydro-electric plant; it is a design that indicates his interest in the structural capabilities of the arch before he began building multiple arch dams. Courtesy Mr. Charles Allan Whitney. - Little Rock Creek Dam, Little Rock Creek, Littlerock, Los Angeles County, CA
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13. Roadway view on 1927 bridge looking north from Crockett ...
13. Roadway view on 1927 bridge looking north from Crockett at on-coming I80 west-bound traffic. Photo taken from Pier 5. - Carquinez Bridge, Spanning Carquinez Strait at Interstate 80, Vallejo, Solano County, CA
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12. VIEW FROM PARKWAY MEDIAN TO SPORT HILL ROAD BRIDGE, ...
12. VIEW FROM PARKWAY MEDIAN TO SPORT HILL ROAD BRIDGE, Copy of photograph ca. 1940. Collection Connecticut Department of Transportation. - Merritt Parkway, Bridge No. 744, Spanning Merritt Parkway at Route 59, Fairfield, Fairfield County, CT
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121. Plan and profile of proposed highway bridge across Carquinez ...
121. Plan and profile of proposed highway bridge across Carquinez Strait. 10/17/1923. The Rodeo-Vallejo Ferry Co., Aven Hanford, President. - Carquinez Bridge, Spanning Carquinez Strait at Interstate 80, Vallejo, Solano County, CA
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11. REPRESENTATIVE VIEW OF DELL AVENUE VEHICULAR BRIDGES, LOOKING WEST ...
11. REPRESENTATIVE VIEW OF DELL AVENUE VEHICULAR BRIDGES, LOOKING WEST FROM SOUTH SIDE OF LINNIE CANAL. THIS PARTICULAR BRIDGE IS LOCATED OVER LINNIE CANAL. - Venice Canals, Community of Venice, Los Angeles, Los Angeles County, CA
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1. HISTORIC PHOTOGRAPH, VIEW OF ROUND HILL ROAD BRIDGE, LOOKING ...
1. HISTORIC PHOTOGRAPH, VIEW OF ROUND HILL ROAD BRIDGE, LOOKING WEST, CA. 1940. CONNECTICUT DEPARTMENT OF TRANSPORTATION. - Merritt Parkway, Round Hill Road Bridge, Spanning Merritt Parkway at 3.5 mile mark, Greenwich, Fairfield County, CT
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6. VIEW FACING EAST ALONG NORTH FACE OF BRIDGE AT ...
6. VIEW FACING EAST ALONG NORTH FACE OF BRIDGE AT CONSTRUCTION DETAILS OF WOOD RAILINGS AND STONE ABUTMENTS. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA
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5. VIEW EAST ACROSS BRIDGE SHOWING RAILING DETAILS AND WATERFALLS ...
5. VIEW EAST ACROSS BRIDGE SHOWING RAILING DETAILS AND WATERFALLS OF THE SOUTH FORK OF THE TUOLUMNE. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA
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Guo, Dong; McCusker, James K
2007-04-16
The synthesis, physical, and spectroscopic properties of a series of metal complexes bridged by the redox-active chloranilate ligand are described. Compounds containing the (CAcat,cat)4- ligand, where (CAcat,cat)4- represents the fully reduced aromatic form of chloranilate, have been prepared by two different routes from H2CA and H4CA starting materials; the corresponding (CAsq,cat)3- analogue was obtained by one-electron oxidation with decamethylferrocenium tetrafluoroborate. Homo- and heterobimetallic complexes containing CrIII and GaIII with chloranilate have been prepared, yielding the following six complexes: [Ga2(tren)2(CAcat,cat)](BPh4)2 (1), [Ga2(tren)2(CAsq,cat)](BPh4)2(BF4) (2), [GaCr(tren)2(CAcat,cat)](BPh4)2 (3), [GaCr(tren)2(CAsq,cat)](BPh4)2(BF4) (4), [Cr2(tren)2(CAcat,cat)] (BPh4)2 (5), and [Cr2(tren)2(CAsq,cat)](BPh4)2(BF4) (6) (where tren is tris(2-aminoethyl)amine). Single-crystal X-ray structures have been obtained for complexes 1, 3, and 5; nearly identical C-C bond distances within the quinoidal ligand confirm the aromatic character of the bridge in each case. Complex 2 exhibits a temperature-independent magnetic moment of microeff = 1.64 +/- 0.04 microB in the solid state between 4 and 350 K, consistent with the CAsq,cat formulation of the ligand and an S = 1/2 ground state for complex 2. Complex 3 exhibits a value of microeff = 3.44 +/- 0.09 microB that is also temperature-independent, indicating an S = 3/2 ground state. Complexes 4-6 are all influenced by Heisenberg spin exchange. The temperature-independent behavior of complexes 4 and 6 indicate the presence of strong antiferromagnetic exchange between the CrIII and the (sq,cat) bridging radical yielding well-isolated ground states of S = 1 and 5/2 for 4 and 6, respectively. In contrast, complex 5 exhibits a weak intramolecular antiferromagnetic exchange interaction between the two CrIII centers (J = -2 cm-1 for H = -2Jŝ1.ŝ2) via superexchange through the diamagnetic CAcat,cat bridge. The absorption spectra of the CAsq,cat-containing complexes exhibit a number of sharp, relatively intense features in fluid solution. Group theoretical arguments coupled with a qualitative ligand-field analysis including the effects of Heisenberg spin exchange suggest that several of the observed transitions are a consequence of exchange interactions in both the ground- and excited-state manifolds of the compounds.
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Inoue, Takahiro; Kobirumaki-Shimozawa, Fuyu; Kagemoto, Tatsuya; Fujii, Teruyuki; Terui, Takako; Kusakari, Yoichiro; Hongo, Kenichi; Morimoto, Sachio; Ohtsuki, Iwao; Hashimoto, Kazuhiro; Fukuda, Norio
2013-10-01
It has been reported that the Frank-Starling mechanism is coordinately regulated in cardiac muscle via thin filament "on-off" equilibrium and titin-based lattice spacing changes. In the present study, we tested the hypothesis that the deletion mutation ΔK210 in the cardiac troponin T gene shifts the equilibrium toward the "off" state and accordingly attenuate the sarcomere length (SL) dependence of active force production, via reduced cross-bridge formation. Confocal imaging in isolated hearts revealed that the cardiomyocytes were enlarged, especially in the longitudinal direction, in ΔK210 hearts, with striation patterns similar to those in wild type (WT) hearts, suggesting that the number of sarcomeres is increased in cardiomyocytes but the sarcomere length remains unaltered. For analysis of the SL dependence of active force, skinned muscle preparations were obtained from the left ventricle of WT and knock-in (ΔK210) mice. An increase in SL from 1.90 to 2.20μm shifted the mid-point (pCa50) of the force-pCa curve leftward by ~0.21pCa units in WT preparations. In ΔK210 muscles, Ca(2+) sensitivity was lower by ~0.37pCa units, and the SL-dependent shift of pCa50, i.e., ΔpCa50, was less pronounced (~0.11pCa units), with and without protein kinase A treatment. The rate of active force redevelopment was lower in ΔK210 preparations than in WT preparations, showing blunted thin filament cooperative activation. An increase in thin filament cooperative activation upon an increase in the fraction of strongly bound cross-bridges by MgADP increased ΔpCa50 to ~0.21pCa units. The depressed Frank-Starling mechanism in ΔK210 hearts is the result of a reduction in thin filament cooperative activation. © 2013.
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Newman, Rhonda A.; Sorensen, Brenda R.; Kilpatrick, Adina M.; Shea, Madeline A.
2014-01-01
Calmodulin (CaM) plays a vital role in calcium homeostasis by allosterically modulating intracellular calcium channels including the homo-tetrameric human Ryanodine Receptor Type 1 (hRyR1). Apo (calcium-free) CaM activates hRyR1 while calcium-saturated CaM inhibits it. Two CaM-binding regions (residues 1975–1999 and 3614–3643) identified in each RyR1 monomer were proposed to allow CaM to bridge adjacent RyR1 subunits. We explored the distinct roles of CaM domains by using fluorescence anisotropy to determine the affinity of CaM1–148 (full-length), CaM1–80 (N-domain) and CaM76–148 (C-domain) for peptides encompassing hRyR1 residues 1975–1999 or 3614–3643. Both CaM1–148 and CaM76–148 associated in a calcium-independent manner with similar affinities for hRyR1(3614–3643)p while CaM1–80 required calcium and bound ~250-fold more weakly. Association of CaM1–148, CaM1–80 and CaM76–148 with hRyR1(1975–1999)p was much less favorable than with hRyR1(3614–3643)p; differences between the two CaM domains were smaller. Equilibrium calcium titrations monitored by steady-state fluorescence demonstrated that both hRyR1 peptides increased the calcium-binding affinity of both CaM domains. These thermodynamic properties support a prior model in which the CaM C-domain associates with RyR1(3614–3643) at low levels of calcium, positioning CaM to rapidly respond to calcium efflux. However, the affinity of the N-domain of CaM for hRyR1(1975–1999)p is insufficient to explain a model in which CaM bridges adjacent RyR1 subunits within the tetramer. This indicates that other protein factors or properties of the tertiary or quaternary structure of hRyR1 contribute to the energetics of CaM-mediated regulation. PMID:25145833
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Dobrunz, L E; Backx, P H; Yue, D T
1995-01-01
We have developed a novel method for measuring steady-state force-[Ca2+]i relations in isolated, membrane-intact rat trabeculae that are microinjected with Fura-2 salt. Twitches are markedly slowed after inhibition of phasic Ca2+ release and uptake from the sarcoplasmic reticulum by addition of cyclopiazonic acid and ryanodine. During relaxation of slowed twitches, force and [Ca2+]i trace a common trajectory in plots of force versus [Ca2+]i, despite very different histories of contraction. The common trajectory thereby provides a high resolution determination of the steady-state relation between force and [Ca2+]i. Using this method, we show that 1 microM isoproterenol, a beta-adrenergic agonist, causes a rightward shift (Hill function K1/2 increased from 0.39 +/- 0.07 microM to 0.82 +/- 0.23 microM, p < 0.02, n = 6) and a decreased slope (nH decreased from 5.4 +/- 1.1 to 4.0 +/- 1.4, p < 0.02) of the steady-state force-[Ca2+]i curve, with no change in maximal force (Fmax = 99.2 +/- 2.2% of control). In contrast, 2 microM EMD 53998, a racemic thiadiazinone derivative, causes a leftward shift (K1/2 decreased from 0.42 +/- 0.02 microM to 0.30 +/- 0.06 microM, p < 0.02, n = 4) with no change in slope of the steady-state force-[Ca2+]i curve, accompanied by a modest increase in maximal force (Fmax = 107.1 +/- 4.6% of control, p < 0.02). To gain mechanistic insight into these modulatory events, we developed a simple model of cooperative thin filament activation that predicts steady-state force-[Ca2+]i relationships. Model analysis suggests that isoproterenol decreases cooperativity arising from nearest-neighbor interactions between regulatory units on the thin filament, without change in the equilibrium constant for Ca2+ binding. In contrast, the effects of EMD 53998 are consistent with an increase in the affinity of strong-binding cross-bridges, without change in either the affinity of troponin C for Ca2+ or cooperative interactions. PMID:7669896
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7. YOSEMITE VALLEY SHUTTLE BUS AT SENTINEL BRIDGE SHUTTLE BUS ...
7. YOSEMITE VALLEY SHUTTLE BUS AT SENTINEL BRIDGE SHUTTLE BUS AND PARKING LOT AREA. LOOKING WNW. GIS: N-37 40 36.2 / W-119 44 45.0 - Yosemite National Park Roads & Bridges, Yosemite Village, Mariposa County, CA
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1988-01-01
The effects of laser-flash photolytic release of ATP from caged ATP [P3- 1(2-nitrophenyl)ethyladenosine-5'-triphosphate] on stiffness and tension transients were studied in permeabilized guinea pig protal vein smooth muscle. During rigor, induced by removing ATP from the relaxed or contracting muscles, stiffness was greater than in relaxed muscle, and electron microscopy showed cross-bridges attached to actin filaments at an approximately 45 degree angle. In the absence of Ca2+, liberation of ATP (0.1-1 mM) into muscles in rigor caused relaxation, with kinetics indicating cooperative reattachment of some cross- bridges. Inorganic phosphate (Pi; 20 mM) accelerated relaxation. A rapid phase of force development, accompanied by a decline in stiffness and unaffected by 20 mM Pi, was observed upon liberation of ATP in muscles that were released by 0.5-1.0% just before the laser pulse. This force increment observed upon detachment suggests that the cross- bridges can bear a negative tension. The second-order rate constant for detachment of rigor cross-bridges by ATP, in the absence of Ca2+, was estimated to be 0.1-2.5 X 10(5) M-1s-1, which indicates that this reaction is too fast to limit the rate of ATP hydrolysis during physiological contractions. In the presence of Ca2+, force development occurred at a rate (0.4 s-1) similar to that of intact, electrically stimulated tissue. The rate of force development was an order of magnitude faster in muscles that had been thiophosphorylated with ATP gamma S before the photochemical liberation of ATP, which indicates that under physiological conditions, in non-thiophosphorylated muscles, light-chain phosphorylation, rather than intrinsic properties of the actomyosin cross-bridges, limits the rate of force development. The release of micromolar ATP or CTP from caged ATP or caged CTP caused force development of up to 40% of maximal active tension in the absence of Ca2+, consistent with cooperative attachment of cross-bridges. Cooperative reattachment of dephosphorylated cross-bridges may contribute to force maintenance at low energy cost and low cross-bridge cycling rates in smooth muscle. PMID:3373178
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Mamidi, Ranganath; Li, Jiayang; Gresham, Kenneth S; Verma, Sujeet; Doh, Chang Yoon; Li, Amy; Lal, Sean; Dos Remedios, Cristobal G; Stelzer, Julian E
2017-10-01
Omecamtiv mecarbil (OM) enhances systolic function in vivo by directly binding the myosin cross-bridges (XBs) in the sarcomere. However, the mechanistic details governing OM-induced modulation of XB behavior in failing human myocardium are unclear. The effects of OM on steady state and dynamic XB behavior were measured in chemically skinned myocardial preparations isolated from human donor and heart failure (HF) left ventricle. HF myocardium exhibited impaired contractile function as evidenced by reduced maximal force, magnitude of XB recruitment ( P df ), and a slowed rate of XB detachment ( k rel ) at submaximal Ca 2+ activations. Ca 2+ sensitivity of force generation (pCa 50 ) was higher in HF myocardium when compared with donor myocardium, both prior to and after OM incubations. OM incubation (0.5 and 1.0 μmol/L) enhanced force generation at submaximal Ca 2+ activations in a dose-dependent manner. Notably, OM induced a slowing in k rel with 1.0 μmol/L OM but not with 0.5 μmol/L OM in HF myocardium. Additionally, OM exerted other differential effects on XB behavior in HF myocardium as evidenced by a greater enhancement in P df and slowing in the time course of cooperative XB recruitment ( T rec ), which collectively prolonged achievement of peak force development ( T pk ), compared with donor myocardium. Our findings demonstrate that OM augments force generation but also prolongs the time course of XB transitions to force-bearing states in remodeled HF myocardium, which may extend the systolic ejection time in vivo. Optimal OM dosing is critical for eliciting enhanced systolic function without excessive prolongation of systolic ejection time, which may compromise diastolic filling. © 2017 American Heart Association, Inc.
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8. VIEW OF ACCESS BRIDGE AND INTAKE PIER FROM THE ...
8. VIEW OF ACCESS BRIDGE AND INTAKE PIER FROM THE BRIDGE PIER ABUTMENT, LOOKING NORTHEAST. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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Kulik, Leonid V; Epel, Boris; Lubitz, Wolfgang; Messinger, Johannes
2007-11-07
The heart of the oxygen-evolving complex (OEC) of photosystem II is a Mn4OxCa cluster that cycles through five different oxidation states (S0 to S4) during the light-driven water-splitting reaction cycle. In this study we interpret the recently obtained 55Mn hyperfine coupling constants of the S0 and S2 states of the OEC [Kulik et al. J. Am. Chem. Soc. 2005, 127, 2392-2393] on the basis of Y-shaped spin-coupling schemes with up to four nonzero exchange coupling constants, J. This analysis rules out the presence of one or more Mn(II) ions in S0 in methanol (3%) containing samples and thereby establishes that the oxidation states of the manganese ions in S0 and S2 are, at 4 K, Mn4(III, III, III, IV) and Mn4(III, IV, IV, IV), respectively. By applying a "structure filter" that is based on the recently reported single-crystal EXAFS data on the Mn4OxCa cluster [Yano et al. Science 2006, 314, 821-825] we (i) show that this new structural model is fully consistent with EPR and 55Mn-ENDOR data, (ii) assign the Mn oxidation states to the individual Mn ions, and (iii) propose that the known shortening of one 2.85 A Mn-Mn distance in S0 to 2.75 A in S1 [Robblee et al. J. Am. Chem. Soc. 2002, 124, 7459-7471] corresponds to a deprotonation of a mu-hydroxo bridge between MnA and MnB, i.e., between the outer Mn and its neighboring Mn of the mu3-oxo bridged moiety of the cluster. We summarize our results in a molecular model for the S0 --> S1 and S1 --> S2 transitions.
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Buchanan, P.A.
2004-01-01
This article presents time-series graphs of specific-conductance and water-temperature data collected in San Francisco Bay during water year 2003 (October 1, 2002, through September 30, 2003). Specific-conductance and water-temperature data were recorded at 15-minute intervals at the following US Geological Survey (USGS) locations (Figure 1): • Suisun Bay at Benicia Bridge, near Benicia, CA. (BEN) (site # 11455780) • Carquinez Strait at Carquinez Bridge, near Crockett, CA. (CARQ) (site # 11455820) • Napa River at Mare Island Causeway, near Vallejo, CA. (NAP) (site # 11458370) • San Pablo Strait at Point San Pablo, CA. (PSP) (site # 11181360) • San Pablo Bay at Petaluma River Channel Marker 9, CA. (SPB) (site # 380519122262901) • San Francisco Bay at Presidio Military Reservation, CA. (PRES) (site # 11162690) • San Francisco Bay at San Mateo Bridge, near Foster City, CA. (SMB) (site # 11162765) Suspended-sediment-concentration data also were collected at most of these sites during water year 2003. Specific-conductance and water-temperature data from PSP, PRES, and SMB were recorded by the CA Department of Water Resources (DWR) before 1988, by the USGS National Research Program from 1988 to 1989, and by the USGS-DWR cooperative program since 1990. BEN, CARQ, NAP, and SPB were established in 1998 by USGS. The monitoring station at PRES was discontinued on November 12, 2002, due to shoaling at the site.
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5. HISTORIC PHOTOGRAPH OF FIRST CHRISTINE FALLS BRIDGE, BUILT BY ...
5. HISTORIC PHOTOGRAPH OF FIRST CHRISTINE FALLS BRIDGE, BUILT BY U.S. ARMY CORPS OF ENGINEERS CA. 1908. PHOTOGRAPHER TAKEN 1911. MOUNT RAINIER NATIONAL PARK ARCHIVES - Christine Falls Bridge, Spanning Van Trump Creek on Nisqually Road, Longmire, Pierce County, WA
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35. BRIDGE, CONSTRUCTION MISSISSIPPI, LOWNDES CO. COLUMBUS End of Main ...
35. BRIDGE, CONSTRUCTION MISSISSIPPI, LOWNDES CO. COLUMBUS End of Main St., Columbus Bridge under construction, 1925-27. Photo from S side of W approach. Credit: Shenks Photography, Columbus, Ms, owner. O. Pruitt, photographer, ca. 1927. Copied by Sarcone Photography, columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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34. BRIDGE, CONSTRUCTION MISSISSIPPI, LOWNDES CO. COLUMBUS End of Main ...
34. BRIDGE, CONSTRUCTION MISSISSIPPI, LOWNDES CO. COLUMBUS End of Main St., Columbus Date: 1925-27. Old bridge in background. Photo taken from such (probably east) bank. Credit: Shenks Photography, Columbus, Ms, owner. O. Pruitt, photographer, ca. 1927. Copied by Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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Tellurite glasses for vitrification of technetium-99 from pyrochemical processing
NASA Astrophysics Data System (ADS)
Pyo, Jae-Young; Lee, Cheong Won; Park, Hwan-Seo; Yang, Jae Hwan; Um, Wooyong; Heo, Jong
2017-09-01
A new alkali-alumino tellurite glass composition was developed to immobilize highly-volatile technetium (Tc) wastes generated from the pyrochemical processing technology. Tellurite glass can incorporate up to 7 mass% of rhenium (Re, used as a surrogate for Tc) with an average retention of 86%. Normalized elemental releases evaluated by seven-day product consistency test (PCT) satisfied the immobilized low activity waste requirements of United States when concentration of Ca(ReO4)2 in the glass was <12 mass%. Re ions form Re7+ and are coordinated with four oxygens to form ReO4- tetrahedra. These tetrahedra bond to modifiers such as Ca2+ or Na+ that are further connected to the tellurite glass network by Ca2+ (or Na+) - non-bridging oxygen bonds.
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Nakamura, Shin; Noguchi, Takumi
2016-10-11
During photosynthesis, the light-driven oxidation of water performed by photosystem II (PSII) provides electrons necessary to fix CO 2 , in turn supporting life on Earth by liberating molecular oxygen. Recent high-resolution X-ray images of PSII show that the water-oxidizing center (WOC) is composed of an Mn 4 CaO 5 cluster with six carboxylate, one imidazole, and four water ligands. FTIR difference spectroscopy has shown significant structural changes of the WOC during the S-state cycle of water oxidation, especially within carboxylate groups. However, the roles that these carboxylate groups play in water oxidation as well as how they should be properly assigned in spectra are unresolved. In this study, we performed a normal mode analysis of the WOC using the quantum mechanics/molecular mechanics (QM/MM) method to simulate FTIR difference spectra on the S 1 to S 2 transition in the carboxylate stretching region. By evaluating WOC models with different oxidation and protonation states, we determined that models of high-oxidation states, Mn(III) 2 Mn(IV) 2 , satisfactorily reproduced experimental spectra from intact and Ca-depleted PSII compared with low-oxidation models. It is further suggested that the carboxylate groups bridging Ca and Mn ions within this center tune the reactivity of water ligands bound to Ca by shifting charge via their π conjugation.
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Calmodulin overexpression does not alter Cav1.2 function or oligomerization state.
Findeisen, Felix; Tolia, Alexandra; Arant, Ryan; Kim, Eun Young; Isacoff, Ehud; Minor, Daniel L
2011-01-01
Interactions between calmodulin (CaM) and voltage-gated calcium channels (Ca(v)s) are crucial for Ca(v) activity-dependent feedback modulation. We recently reported an X-ray structure that shows two Ca(2+)/CaM molecules bound to the Ca(v)1.2 C terminal tail, one at the PreIQ region and one at the IQ domain. Surprisingly, the asymmetric unit of the crystal showed a dimer in which Ca(2+)/CaM bridged two PreIQ helixes to form a 4:2 Ca(2+)/CaM:Ca(v) C-terminal tail assembly. Contrary to previous proposals based on a similar crystallographic dimer, extensive biochemical analysis together with subunit counting experiments of full-length channels in live cell membranes failed to find evidence for multimers that would be compatible with the 4:2 crossbridged complex. Here, we examine this possibility further. We find that CaM over-expression has no functional effect on Ca(v)1.2 inactivation or on the stoichiometry of full-length Ca(v)1.2. These data provide further support for the monomeric Ca(v)1.2 stoichiometry. Analysis of the electrostatic surfaces of the 2:1 Ca(2+)/CaM:Ca(V) C-terminal tail assembly reveals notable patches of electronegativity. These could influence various forms of channel modulation by interacting with positively charged elements from other intracellular channel domains.
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Plans: Aft Gun Platform, Quarters for 16 Gunmen, Poop Deck, ...
Plans: Aft Gun Platform, Quarters for 16 Gunmen, Poop Deck, Boat Deck, House Top, Bridge Deck, Upper Bridge Deck, Navigating Bridge, Forecastle Deck, Gun Platform, Upper Deck, Second Deck and Hold Plan - Mission Santa Ynez, Suisun Bay Reserve Fleet, Benicia, Solano County, CA
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9. Terminal connection of arch structural member to concrete abutment ...
9. Terminal connection of arch structural member to concrete abutment on east of south end of bridge. Slightly oblique detail view west-northwest (from beside bridge). 150 mm lens. - Gault Bridge, Spanning Deer Creek at South Pine Street, Nevada City, Nevada County, CA
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11. Connection of upright structural members to top of arch ...
11. Connection of upright structural members to top of arch member on east side of north end of bridge. Oblique detail view southwest (from beside bridge). 360 mm lens. - Gault Bridge, Spanning Deer Creek at South Pine Street, Nevada City, Nevada County, CA
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DOE Office of Scientific and Technical Information (OSTI.GOV)
Fallon, Jennifer L.; Baker, Mariah R.; Xiong, Liangwen
2009-11-10
Voltage-dependent calcium channels (Ca(V)) open in response to changes in membrane potential, but their activity is modulated by Ca(2+) binding to calmodulin (CaM). Structural studies of this family of channels have focused on CaM bound to the IQ motif; however, the minimal differences between structures cannot adequately describe CaM's role in the regulation of these channels. We report a unique crystal structure of a 77-residue fragment of the Ca(V)1.2 alpha(1) subunit carboxyl terminus, which includes a tandem of the pre-IQ and IQ domains, in complex with Ca(2+).CaM in 2 distinct binding modes. The structure of the Ca(V)1.2 fragment is anmore » unusual dimer of 2 coiled-coiled pre-IQ regions bridged by 2 Ca(2+).CaMs interacting with the pre-IQ regions and a canonical Ca(V)1-IQ-Ca(2+).CaM complex. Native Ca(V)1.2 channels are shown to be a mixture of monomers/dimers and a point mutation in the pre-IQ region predicted to abolish the coiled-coil structure significantly reduces Ca(2+)-dependent inactivation of heterologously expressed Ca(V)1.2 channels.« less
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18. INTAKE PIER, BRIDGE DETAILS, SHEET 9 OF 117, 1920. ...
18. INTAKE PIER, BRIDGE DETAILS, SHEET 9 OF 117, 1920. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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10. Hingepin connection of arch structural member to concrete footing ...
10. Hinge-pin connection of arch structural member to concrete footing on east of south end of bridge. Slightly oblique detail view west-northwest (from beside bridge). 150 mm lens. - Gault Bridge, Spanning Deer Creek at South Pine Street, Nevada City, Nevada County, CA
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1. West end of Bridge Number 210.52, oblique contextual view ...
1. West end of Bridge Number 210.52, oblique contextual view to northwest showing the downstream side, 90mm lens. The long trestle approach to the west end of this bridge precluded safe accessibility for axial photography of the west portal. - Southern Pacific Railroad Shasta Route, Bridge No. 210.52, Milepost 210.52, Tehama, Tehama County, CA
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Bai, Fan; Groth, Heather L; Kawai, Masataka
2012-01-01
Two DCM mutants (E40K and E54K) of tropomyosin (Tm) were examined using the thin-filament extraction/reconstitu-tion technique. The effects of the Ca²⁺, ATP, phos-phate (Pi), and ADP concentrations on isometric tension and its transients were studied at 25°C, and the results were com-pared to those for the WT protein. Our results indicate that both E40K and E54K have a significantly lower T(HC) (high Ca²⁺ ten-sion at pCa 4.66) (E40K: 1.21±0.06 T(a), ±SEM, N = 34; E54K: 1.24±0.07 T(a), N = 28), a significantly lower T(LC) (low- Ca²⁺ tension at pCa 7.0) (E40K: 0.07±0.02 T(a), N = 34; E54K: 0.06±0.02 T(a), N = 28), and a significantly lower T(act) (Ca²⁺ activatable tension) (T(act) = T(HC)-T(LC,) E40K: 1.15±0.08 T(a), N = 34; E54K: 1.18±0.06 T(a), N = 28) than WT (T(HC) = 1.53±0.07 T(a), T(LC) = 0.12±0.01 T(a), T(act) = 1.40±0.07 T(a), N = 25). All tensions were normalized to T(a) ( = 13.9±0.8 kPa, N = 57), the ten-sion of actin-filament reconstituted cardiac fibers (myocardium) under the standard activating conditions. The Ca²⁺ sensitivity (pCa₅₀) of E40K (5.23±0.02, N = 34) and E54K (5.24±0.03, N = 28) was similar to that of the WT protein (5.26±0.03, N = 25). The cooper-a-tivity increased significantly in E54K (3.73±0.25, N = 28) compared to WT (2.80±0.17, N = 25). Seven kinetic constants were deduced using sinusoidal analysis at pCa 4.66. These results enabled us to calculate the cross-bridge distribution in the strongly attached states, and thereby deduce the force/cross-bridge. The results indicate that the force/cross-bridge is ∼15% less in E54K than WT, but remains similar to that of the WT protein in the case of E40K. We conclude that over-inhibition of the actomyosin interaction by E40K and E54K Tm mutants leads to a decreased force-generating ability at systole, which is the main mechanism underlying the early pathogenesis of DCM.
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44. MISSISSIPPI, LOWNDES CO. COLUMBUS RAILROAD BRIDGE End of 1st ...
44. MISSISSIPPI, LOWNDES CO. COLUMBUS RAILROAD BRIDGE End of 1st St. S., Columbus, Ms. Detail from Camille Drie's map: A Bird's Eye View of Columbus, Mississippi ca. 1875-76. Shows M&O RR bridge before the Phoenix Bridge Co. erected iron truss spans in 1878. Credit: Photostat of map in Lowndes Co. Public Library Sarcone Photography, Columbus, Ms. 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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17. Baltimore through truss steel bridge (1905), built by the ...
17. Baltimore through truss steel bridge (1905), built by the American Bridge Company. The bridge is 15 to 20 feet wide, with a wooden deck, and connects the Sullivan Machine Co. with the Foundry. The enclosed bridge in the background was constructed ca. 1920, and connects the Chain Machine Building with its power plant, foundry, and pattern shop. - Sullivan Machinery Company, Main Street between Pearl & Water Streets, Claremont, Sullivan County, NH
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Gorvin, Caroline M; Babinsky, Valerie N; Malinauskas, Tomas; Nissen, Peter H; Schou, Anders J; Hanyaloglu, Aylin C; Siebold, Christian; Jones, E Yvonne; Hannan, Fadil M; Thakker, Rajesh V
2018-02-20
The calcium-sensing receptor (CaSR) is a G protein-coupled receptor (GPCR) that signals through G q/11 and G i/o to stimulate cytosolic calcium (Ca 2+ i ) and mitogen-activated protein kinase (MAPK) signaling to control extracellular calcium homeostasis. Studies of loss- and gain-of-function CASR mutations, which cause familial hypocalciuric hypercalcemia type 1 (FHH1) and autosomal dominant hypocalcemia type 1 (ADH1), respectively, have revealed that the CaSR signals in a biased manner. Thus, some mutations associated with FHH1 lead to signaling predominantly through the MAPK pathway, whereas mutations associated with ADH1 preferentially enhance Ca 2+ i responses. We report a previously unidentified ADH1-associated R680G CaSR mutation, which led to the identification of a CaSR structural motif that mediates biased signaling. Expressing CaSR R680G in HEK 293 cells showed that this mutation increased MAPK signaling without altering Ca 2+ i responses. Moreover, this gain of function in MAPK activity occurred independently of G q/11 and G i/o and was mediated instead by a noncanonical pathway involving β-arrestin proteins. Homology modeling and mutagenesis studies showed that the R680G CaSR mutation selectively enhanced β-arrestin signaling by disrupting a salt bridge formed between Arg 680 and Glu 767 , which are located in CaSR transmembrane domain 3 and extracellular loop 2, respectively. Thus, our results demonstrate CaSR signaling through β-arrestin and the importance of the Arg 680 -Glu 767 salt bridge in mediating signaling bias. Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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... with Williams syndrome may show: A flattened nasal bridge with small upturned nose Long ridges in the ... Alternative Names Williams-Beuren syndrome Images Low nasal bridge Chromosomes and DNA References Morris CA. Williams syndrome. ...
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2. OVERALL VIEW OF INTAKE PIER AND ACCESS BRIDGE, LOOKING ...
2. OVERALL VIEW OF INTAKE PIER AND ACCESS BRIDGE, LOOKING NORTH. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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4. DETAIL OF THE BRIDGE PIER SHOWING THE SUSPENSION CABLE, ...
4. DETAIL OF THE BRIDGE PIER SHOWING THE SUSPENSION CABLE, LOOKING SOUTHWEST. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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3. APPROACH TO THE ACCESS BRIDGE AND INTAKE PIER, LOOKING ...
3. APPROACH TO THE ACCESS BRIDGE AND INTAKE PIER, LOOKING SOUTHWEST. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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17. INTAKE PIER, BRIDGE STRESS SHEET, SHEET 8 OF 117, ...
17. INTAKE PIER, BRIDGE STRESS SHEET, SHEET 8 OF 117, 1920. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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37. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway ...
37. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway Department, photographer, ca. July 1928 (original print located at Arizona Department of Transportation, Phoenix AZ). CONSTRUCTION ON THIRD PANEL OF NORTH ARM. - Navajo Bridge, Spanning Colorado River at U.S. Highway 89 Alternate, Page, Coconino County, AZ
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40. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway ...
40. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway Department, photographer, ca. July 1928 (original print located at Arizona Department of Transportation, Phoenix AZ). CONSTRUCTION OF NORTH ARM, FROM SOUTH ARM. - Navajo Bridge, Spanning Colorado River at U.S. Highway 89 Alternate, Page, Coconino County, AZ
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34. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway ...
34. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway Department, photographer, ca. May 1928 (original print located at Arizona Department of Transportation, Phoenix AZ). CONSTRUCTION ON EIGHT PANEL OF SOUTH ARM. - Navajo Bridge, Spanning Colorado River at U.S. Highway 89 Alternate, Page, Coconino County, AZ
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33. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway ...
33. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway Department, photographer, ca. May 1928 (original print located at Arizona Department of Transportation, Phoenix AZ). CONSTRUCTION OF SOUTH ARM, SHOWING ERECTION TRAVELER. - Navajo Bridge, Spanning Colorado River at U.S. Highway 89 Alternate, Page, Coconino County, AZ
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Plans: 3'/50 Cal. Gun Platform, Boat Deck House Top & ...
Plans: 3'/50 Cal. Gun Platform, Boat Deck House Top & Fidley Top, Boat Deck, Radar Platform Lower, Pilot House Top, Navigation Bridge, Upper Bridge Deck, Poop Deck, Cargo Deck, Motor Boat Platform, Bridge Deck, Forecastle Deck, Upper Deck - Taluga, Suisun Bay Reserve Fleet, Benicia, Solano County, CA
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Imani, Mehdi; Hosseinkhani, Saman; Ahmadian, Shahin; Nazari, Mahboobeh
2010-08-01
The thermal sensitivity and pH-sensitive spectral properties of firefly luciferase have hampered its application in a variety of fields. It is proposed that the stability of a protein can be increased by introduction of disulfide bridge that decreases the configurational entropy of unfolding. A disulfide bridge is introduced into Photinus pyralis firefly luciferase to make two separate mutant enzymes with a single bridge. Even though the A103C/S121C mutant showed remarkable thermal stability, its specific activity decreased, whereas the A296C/A326C mutant showed tremendous thermal stability, relative pH insensitivity and 7.3-fold increase of specific activity. Moreover, the bioluminescence emission spectrum of A296C/A326C was resistant against higher temperatures (37 degrees C). Far-UV CD analysis showed slight secondary structure changes for both mutants. Thermal denaturation analysis showed that conformational stabilities of A103C/S121C and A296C/A326C are more than native firefly luciferase. It is proposed that since A296 and A326 are situated in the vicinity of the enzyme active site microenvironment in comparison with A103 and S121, the formation of a disulfide bridge in this region has more impact on enzyme kinetic characteristics.
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77 FR 10371 - Drawbridge Operation Regulation; Sacramento River, Sacramento, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2012-02-22
... Operation Regulation; Sacramento River, Sacramento, CA AGENCY: Coast Guard, DHS. ACTION: Notice of temporary..., mile 59.0, at Sacramento, CA. The deviation is necessary to allow the bridge owner to conduct... change to the operation of the Tower Drawbridge, mile 59.0, Sacramento River, at Sacramento, CA. The...
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42. MISSISSIPPI, LOWNDES CO. COLUMBUS RAILROAD BRIDGE End of 1st ...
42. MISSISSIPPI, LOWNDES CO. COLUMBUS RAILROAD BRIDGE End of 1st St. S., Columbus, Ms. Copy of postcard ca. 1900. Copy owned and made by Jack Donnell, Columbus, Ms. Shows two-span steel truss, built by Phoenix Bridge Co. in 1878. Negative copied by: Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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6. East portal of Bridge Number 210.52, contextual view to ...
6. East portal of Bridge Number 210.52, contextual view to east, 210mm lens. This structure underscores the inconsistencies with the railroad's use of 'east' and 'west' as opposed to actual direction; in this case the railroad 'east' end of the bridge faces actual west. - Southern Pacific Railroad Shasta Route, Bridge No. 210.52, Milepost 210.52, Tehama, Tehama County, CA
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NASA Astrophysics Data System (ADS)
Perrier, M.; Gallud, A.; Ayadi, A.; Kennouche, S.; Porredon, C.; Gary-Bobo, M.; Larionova, J.; Goze-Bac, Ch.; Zanca, M.; Garcia, M.; Basile, I.; Long, J.; de Lapuente, J.; Borras, M.; Guari, Y.
2015-07-01
Cyano-bridged Gd3+/[Fe(CN)6]3- coordination polymer nanoparticles of 3-4 nm stabilized with d-mannitol presenting a high r1 relaxivity value of 11.4 mM-1 s-1 were investigated in vivo as contrast agents (CA) for Magnetic Resonance Imaging (MRI). They allow an increase of the MR image contrast and can act as an efficient intravascular T1 CA with a relatively long blood-circulation lifetime (60 min) without specific toxicity.Cyano-bridged Gd3+/[Fe(CN)6]3- coordination polymer nanoparticles of 3-4 nm stabilized with d-mannitol presenting a high r1 relaxivity value of 11.4 mM-1 s-1 were investigated in vivo as contrast agents (CA) for Magnetic Resonance Imaging (MRI). They allow an increase of the MR image contrast and can act as an efficient intravascular T1 CA with a relatively long blood-circulation lifetime (60 min) without specific toxicity. Electronic supplementary information (ESI) available: Experimental details and procedures, toxicological data, physical characterization. See DOI: 10.1039/c5nr01557j
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5. OBLIQUE VIEW OF INTAKE PIER AND ACCESS BRIDGE, LOOKING ...
5. OBLIQUE VIEW OF INTAKE PIER AND ACCESS BRIDGE, LOOKING SOUTHWEST, FROM THE EASTERN LEVEE. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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1. OVERALL VIEW OF INTAKE PIER AND ACCESS BRIDGE, LOOKING ...
1. OVERALL VIEW OF INTAKE PIER AND ACCESS BRIDGE, LOOKING NORTHEAST FROM WESTERN LEVEE OF THE SACRAMENTO RIVER. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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15. 'Southern Pacific R.R., One 236'0' Bet. End Pins S. ...
15. 'Southern Pacific R.R., One 236'-0' Bet. End Pins S. Tr. Thro'. Draw Bridge over Sacramento River at Tehama, The Phoenix Bridge Co., Phoenixville, Pa., Jan'y 19th 98, Dwg. 585.' - Southern Pacific Railroad Shasta Route, Bridge No. 210.52, Milepost 210.52, Tehama, Tehama County, CA
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38. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway ...
38. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway Department, photographer, ca. July 1928 (original print located at Arizona Department of Transportation, Phoenix AZ). VIEW FROM CANYON OF THIRD PANEL OF NORTH ARM UNDER CONSTRUCTION. - Navajo Bridge, Spanning Colorado River at U.S. Highway 89 Alternate, Page, Coconino County, AZ
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INTERIOR OF SECOND FLOOR BRIDGE BETWEEN PLANING MILL AND CAR ...
INTERIOR OF SECOND FLOOR BRIDGE BETWEEN PLANING MILL AND CAR MACHINE SHOP, LOOKING SOUTH TOWARD PLANING MILL. - Southern Pacific, Sacramento Shops, Planing Mill, 111 I Street, Sacramento, Sacramento County, CA
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38. MISSISSIPPI, LOWNDES CO. COLUMBUS OLD ROAD BRIDGE End of ...
38. MISSISSIPPI, LOWNDES CO. COLUMBUS OLD ROAD BRIDGE End of Main St., Columbus Show/fabrication details of patented arch truss of Wrought Iron Bridge Co., Canton, Ohio. Taken from middle of swing span looking W toward arch span. Credit: Shenks Photography, Columbus, Ms, owner. O. Pruitt, photographer, ca. 1927-28. Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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6. Oblique view of upstream side of Bridge Number 310.58, ...
6. Oblique view of upstream side of Bridge Number 310.58, 135mm lens. Note ashlar stone masonry abutment built in 1886, Tunnel 15 at left. Heavy vegetation cover, steep banks, and lack of streamside footing precluded full elevation views of the upstream and downstream sides of this bridge. - Southern Pacific Railroad Shasta Route, Bridge No. 310.58, Milepost 310.58, Sims, Shasta County, CA
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A molecular dynamics study of the atomic structure of (CaO)x(SiO2)1-x glasses.
Mead, Robert N; Mountjoy, Gavin
2006-07-27
The local atomic environment of Ca in (CaO)x(SiO2)1-x glasses is of interest because of the role of Ca in soda-lime glass, the application of calcium silicate glasses as biomaterials, and the previous experimental measurement of the Ca-Ca correlation in CaSiO(3) glass. Molecular dynamics has been used to obtain models of (CaO)x(SiO2)1-x glasses with x = 0, 0.1, 0.2, 0.3, 0.4, and 0.5, and with approximately 1000 atoms and size approximately 25 A. As expected, the models contain a tetrahedral silica network, the connectivity of which decreases as x increases. In the glass-forming region, i.e., x = 0.4 and 0.5, Ca has a mixture of 6- and 7-fold coordination. Bridging oxygen makes an important contribution to the coordination of Ca, with most bridging oxygens coordinated to 2 Si plus 1 Ca. The x = 0.5 model is in reasonable agreement with previous experimental studies, and does not substantiate the previous theory of cation ordering, which predicted Ca arranged in sheets. In the phase-separated region, i.e., x = 0.1 and 0.2, there is marked clustering of Ca.
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Altered myocardial force generation in end-stage human heart failure.
Papp, Zoltán; van der Velden, Jolanda; Borbély, Attila; Édes, István; Stienen, Ger J M
2014-12-01
This study aimed to elucidate the molecular background of increased Ca 2+ sensitivity of force production in cardiomyocytes of end-stage human heart failure. Ca 2+ -activated isometric force and the cross-bridge specific rate of force redevelopment (k tr ) were determined in Triton-skinned myocytes from end-stage failing and non-failing donor hearts. Measurements (control: pH 7.2, 0 mM inorganic phosphate (P i )) were performed under test conditions that probed either the Ca 2+ -regulatory function of the thin filaments (pH 6.5), the kinetics of the actin-myosin cross-bridge cycle (10 mM P i ), or both (pH 6.5, 10 mM P i ). The control maximal Ca 2+ -activated force (F o ) and k trmax did not differ between failing and non-failing myocytes. At submaximal [Ca 2+ ], however, both force and k tr were higher in failing than in donor myocytes. The difference in the Ca 2+ sensitivities of force production was preserved when the thin filament regulatory function was perturbed by acidosis (pH 6.5) but was abolished by cross-bridge modulation (i.e. by P i ) both at pH 7.2 and at pH 6.5. P i induced a larger reduction in force but a smaller increase in k tr in the failing myocytes than in the non-failing myocytes at submaximal [Ca 2+ ]. The enhanced P i sensitivity of the actin-myosin interaction suggests that the P i release step of the actin-myosin cross-bridge cycle is modified during end-stage human heart failure. This might be of functional importance when P i accumulates (e.g. during cardiac ischaemia). Moreover, this alteration can influence cardiac energetics and the clinical efficacy of sarcomere targeted agents in human heart failure. © 2015 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of the European Society of Cardiology.
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4. THREE HISTORIC PHOTOGRAPHS, VIEWS OF STONEWORK BEING LAID FOR ...
4. THREE HISTORIC PHOTOGRAPHS, VIEWS OF STONEWORK BEING LAID FOR THE GUINEA ROAD BRIDGE, CA. 1935. COLLECTION CONNECTICUT DEPARTMENT OF TRANSPORTATION. - Merritt Parkway, Guinea Road Bridge, Spanning Merritt Parkway, Stamford, Fairfield County, CT
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76 FR 45421 - Drawbridge Operation Regulation; China Basin, San Francisco, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2011-07-29
... participate in the scheduled San Francisco Marathon, a community event. This deviation allows the bridge to... Francisco Marathon to cross the bridge during the event. This temporary deviation has been coordinated with...
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37. MISSISSIPPI, LOWNDES CO. COLUMBUS BRIDGE, CONSTRUCTION End of Main ...
37. MISSISSIPPI, LOWNDES CO. COLUMBUS BRIDGE, CONSTRUCTION End of Main St., Columbus Overhead view of round, swing pier, showing steel reinforcing rods, workmen. During construction, 1925-27. Credit: Shenks Photography, Columbus, Ms, owner. O. Pruitt, photographer, ca. 1926. Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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33. BRIDGE, CONSTRUCTION MISSISSIPPI, LOWNDES CO. COLUMBUS End of Main ...
33. BRIDGE, CONSTRUCTION MISSISSIPPI, LOWNDES CO. COLUMBUS End of Main St., Columbus Center and east pier, with framing, makes panorama with preceding photo. Date: 1925-27. Credit: Shenks Photographi, Columbus, Ms. owner. O. Pruitt, photographer, ca. 1926 Copied by Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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36. MISSISSIPPI, LOWNDES CO. COLUMBUS Tombigbee R. NEW & OLD ...
36. MISSISSIPPI, LOWNDES CO. COLUMBUS Tombigbee R. NEW & OLD HIGHWAY BRIDGES End of Main St., Columbus Photo, 1927-28, after new bridge (foreground) was completed. From SW bank. Credit: Shenks Photography, Columbus, Ms. owner. O. Pruitt, photographer, ca. 1928. Copied by Sarcone Photography, Columbus, Ms. Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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39. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway ...
39. Photocopy of photograph, R.A. Hoffman, Bridge Engineer, Arizona Highway Department, photographer, ca. July 1928 (original print located at Arizona Department of Transportation, Phoenix AZ). ASSEMBLY OF TRAVELER ON NORTH ARM, SHOWING TEMPORARY TIEBACKS AND ANCHORAGE ARMS. - Navajo Bridge, Spanning Colorado River at U.S. Highway 89 Alternate, Page, Coconino County, AZ
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17. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...
17. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919," showing plan of bars in top flange, elevation of girder reinforcement, plan of bars in bottom flange - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA
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15. 'Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...
15. 'Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919,' showing general plan, plan of top chord, elevation of main girder, transverse section, plan section at deck level. - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA
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18. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...
18. "Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919," showing elevation of center pier, elevation and plan of north and south abutments, sections of abutments, pier, and pier footings - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA
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16. 'Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, ...
16. 'Concrete Bridge Over Salt River, Port Kenyon, Humboldt County, California, A.J. Logan, County Surveyor, H.J. Brunnier, Consulting Engineer, March 7, 1919,' showing detail of floor beam at central pier, half section of cantilever slab at end of bridge, floor beam end panels, slab reinforcing, plan of slab reinforcing, diagram of slab bars, typical floor girder. - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA
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188. Frank Deras Jr., Photographer April 1998 VIEW OF SUSPENSION ...
188. Frank Deras Jr., Photographer April 1998 VIEW OF SUSPENSION BRIDGE, SOUTH SIDE, WITH SAN FRANCISCO IN BACKGROUND, FACING WEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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Sacramento River Water Treatment Plant Intake Pier & Access Bridge, ...
Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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7. Contextual view to eastnortheast showing downstream (west) side of ...
7. Contextual view to east-northeast showing downstream (west) side of bridge in setting, depicting dense riparian nature of area. - Stanislaus River Bridge, Atchison, Topeka & Santa Fe Railway at Stanislaus River, Riverbank, Stanislaus County, CA
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Campbell, Kenneth S; Moss, Richard L
2000-01-01
Paired ramp stretches and releases (‘triangular length changes’, typically 0.04 ± 0.09L0 s−1; mean ±s.e.m.) were imposed on permeabilised rabbit psoas fibre segments under sarcomere length control. In actively contracting fibres, the tension response to stretch was biphasic; tension rose more rapidly during the first 0.005L0 of the imposed stretch than thereafter. Tension also dropped in a biphasic manner during shortening, and at the end of the length change was reduced below the steady state. If a second triangular length change was imposed shortly after the first, tension rose less sharply during the initial phase of lengthening, i.e. the stiffness of the muscle during the initial phase of the response was reduced in the second stretch. This is a thixotropic effect. If a third triangular length change was imposed on the muscle, the response was the same as that to the second. The time required to recover the original tension response was measured by varying the interval between triangular length changes. Recovery to steady state occurred at a rate of ∼1 s−1. The stiffness of the muscle during the initial phase of the response scaled with the developed tension in pCa (=−log10[Ca2+]) solutions ranging from 6.3 (minimal activation) to 4.5 (saturating effect). The relative thixotropic reduction in stiffness measured using paired length changes was independent of the pCa of the activating solution. The thixotropic behaviour of contracting skeletal muscle can be explained by a cross-bridge model of muscle contraction in which the number of attached cross-bridges is temporarily reduced following an imposed movement. PMID:10835052
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11. VIEW OF ARROYO SECO CHANNEL SEEN FROM AVENUE 14 ...
11. VIEW OF ARROYO SECO CHANNEL SEEN FROM AVENUE 14 BRIDGE. SAN FERNANDO ROAD BRIDGE AT CENTER. LOOKING 25° NNE. - Arroyo Seco Parkway, Figueroa Street Viaduct, Spanning Los Angeles River, Los Angeles, Los Angeles County, CA
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10. PARTIAL VIEW OF SOUTH FACE OF MST AND BRIDGE ...
10. PARTIAL VIEW OF SOUTH FACE OF MST AND BRIDGE CRANE MOVED TO ITS SOUTHERN LIMIT - Vandenberg Air Force Base, Space Launch Complex 3, Launch Pad 3 West, Napa & Alden Roads, Lompoc, Santa Barbara County, CA
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11. PARTIAL VIEW OF NORTH FACE OF MST AND BRIDGE ...
11. PARTIAL VIEW OF NORTH FACE OF MST AND BRIDGE CRANE MOVED TO ITS NORTHERN LIMIT - Vandenberg Air Force Base, Space Launch Complex 3, Launch Pad 3 West, Napa & Alden Roads, Lompoc, Santa Barbara County, CA
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2. VIEW OF NORTH FACE SHOWING SUBSTRUCTURE AND ABUTMENTS OF ...
2. VIEW OF NORTH FACE SHOWING SUBSTRUCTURE AND ABUTMENTS OF BRIDGE CROSSING THE SOUTH FORK OF THE TUOLUMNE RIVER. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA
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12. Railing and newel post detail on west side of ...
12. Railing and newel post detail on west side of south end of bridge. Oblique view northwest (from roadway). 90 mm lens. - Gault Bridge, Spanning Deer Creek at South Pine Street, Nevada City, Nevada County, CA
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37. View of the control house on the north tower ...
37. View of the control house on the north tower from the north span facing north. Note mirror and video camera used by bridge operator to check for vessel traffic prior to operating the bridge, loudspeaker and sirens to warn pedestrians and boaters. - Henry Ford Bridge, Spanning Cerritos Channel, Los Angeles-Long Beach Harbor, Los Angeles, Los Angeles County, CA
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DOE Office of Scientific and Technical Information (OSTI.GOV)
Colson, Brett A.; Locher, Matthew R.; Bekyarova, Tanya
2010-05-25
Phosphorylation of myosin regulatory light chain (RLC) by myosin light chain kinase (MLCK) and myosin binding protein-C (cMyBP-C) by protein kinase A (PKA) independently accelerate the kinetics of force development in ventricular myocardium. However, while MLCK treatment has been shown to increase the Ca{sup 2+} sensitivity of force (pCa{sub 50}), PKA treatment has been shown to decrease pCa{sub 50}, presumably due to cardiac troponin I phosphorylation. Further, MLCK treatment increases Ca{sup 2+}-independent force and maximum Ca{sup 2+}-activated force, whereas PKA treatment has no effect on either force. To investigate the structural basis underlying the kinase-specific differential effects on steady-state force,more » we used synchrotron low-angle X-ray diffraction to compare equatorial intensity ratios (I{sub 1,1}/I{sub 1,0}) to assess the proximity of myosin cross-bridge mass relative to actin and to compare lattice spacings (d{sub 1,0}) to assess the inter-thick filament spacing in skinned myocardium following treatment with either MLCK or PKA. As we showed previously, PKA phosphorylation of cMyBP-C increases I{sub 1,1}/I{sub 1,0} and, as hypothesized, treatment with MLCK also increased I{sub 1,1}/I{sub 1,0}, which can explain the accelerated rates of force development during activation. Importantly, interfilament spacing was reduced by {approx}2 nm ({Delta} 3.5%) with MLCK treatment, but did not change with PKA treatment. Thus, RLC or cMyBP-C phosphorylation increases the proximity of cross-bridges to actin, but only RLC phosphorylation affects lattice spacing, which suggests that RLC and cMyBP-C modulate the kinetics of force development by similar structural mechanisms; however, the effect of RLC phosphorylation to increase the Ca{sup 2+} sensitivity of force is mediated by a distinct mechanism, most probably involving changes in interfilament spacing.« less
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Yang, Jianjun; Wang, Jian; Pan, Weinan; Regier, Tom; Hu, Yongfeng; Rumpel, Cornelia; Bolan, Nanthi; Sparks, Donald
2016-01-01
Organic carbon (OC) stability in tropical soils is strongly interlinked with multivalent cation interaction and mineral association. Low molecular weight organic acids (LMWOAs) represent the readily biodegradable OC. Therefore, investigating retention mechanisms of LMWOAs in mineral-cation-LMWOAs systems is critical to understanding soil C cycling. Given the general acidic conditions and dominance of kaolinite in tropical soils, we investigated the retention mechanisms of citric acid (CA) in kaolinite-Fe(III)-CA systems with various Fe/CA molar ratios at pH ~3.5 using Fe K-edge EXAFS and L3,2-edge XANES techniques. With Fe/CA molar ratios >2, the formed ferrihydrite mainly contributed to CA retention through adsorption and/or coprecipitation. With Fe/CA molar ratios from 2 to 0.5, ternary complexation of CA to kaolinite via a five-coordinated Fe(III) bridge retained higher CA than ferrihydrite-induced adsorption and/or coprecipitation. With Fe/CA molar ratios ≤0.5, kaolinite-Fe(III)-citrate complexation preferentially occurred, but less CA was retained than via outer-sphere kaolinite-CA complexation. This study highlighted the significant impact of varied Fe/CA molar ratios on CA retention mechanisms in kaolinite-Fe(III)-CA systems under acidic conditions, and clearly showed the important contribution of Fe-bridged ternary complexation on CA retention. These findings will enhance our understanding of the dynamics of CA and other LMWOAs in tropical soils. PMID:27212680
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DOE Office of Scientific and Technical Information (OSTI.GOV)
Yang, Jianjun; Wang, Jian; Pan, Weinan
Organic carbon (OC) stability in tropical soils is strongly interlinked with multivalent cation interaction and mineral association. Low molecular weight organic acids (LMWOAs) represent the readily biodegradable OC. Therefore, investigating retention mechanisms of LMWOAs in mineral-cation-LMWOAs systems is critical to understanding soil C cycling. Given the general acidic conditions and dominance of kaolinite in tropical soils, we investigated the retention mechanisms of citric acid (CA) in kaolinite-Fe(III)-CA systems with various Fe/CA molar ratios at pH ~3.5 using Fe K-edge EXAFS and L- 3,2-edge XANES techniques. With Fe/CA molar ratios >2, the formed ferrihydrite mainly contributed to CA retention through adsorptionmore » and/or coprecipitation. With Fe/CA molar ratios from 2 to 0.5, ternary complexation of CA to kaolinite via a five-coordinated Fe(III) bridge retained higher CA than ferrihydrite-induced adsorption and/or coprecipitation. With Fe/CA molar ratios ≤ 0.5, kaolinite-Fe(III)-citrate complexation preferentially occurred, but less CA was retained than via outer-sphere kaolinite-CA complexation. This study highlighted the significant impact of varied Fe/CA molar ratios on CA retention mechanisms in kaolinite-Fe(III)-CA systems under acidic conditions, and clearly showed the important contribution of Fe-bridged ternary complexation on CA retention. In conclusion, these findings will enhance our understanding of the dynamics of CA and other LMWOAs in tropical soils.« less
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Yang, Jianjun; Wang, Jian; Pan, Weinan; ...
2016-05-23
Organic carbon (OC) stability in tropical soils is strongly interlinked with multivalent cation interaction and mineral association. Low molecular weight organic acids (LMWOAs) represent the readily biodegradable OC. Therefore, investigating retention mechanisms of LMWOAs in mineral-cation-LMWOAs systems is critical to understanding soil C cycling. Given the general acidic conditions and dominance of kaolinite in tropical soils, we investigated the retention mechanisms of citric acid (CA) in kaolinite-Fe(III)-CA systems with various Fe/CA molar ratios at pH ~3.5 using Fe K-edge EXAFS and L- 3,2-edge XANES techniques. With Fe/CA molar ratios >2, the formed ferrihydrite mainly contributed to CA retention through adsorptionmore » and/or coprecipitation. With Fe/CA molar ratios from 2 to 0.5, ternary complexation of CA to kaolinite via a five-coordinated Fe(III) bridge retained higher CA than ferrihydrite-induced adsorption and/or coprecipitation. With Fe/CA molar ratios ≤ 0.5, kaolinite-Fe(III)-citrate complexation preferentially occurred, but less CA was retained than via outer-sphere kaolinite-CA complexation. This study highlighted the significant impact of varied Fe/CA molar ratios on CA retention mechanisms in kaolinite-Fe(III)-CA systems under acidic conditions, and clearly showed the important contribution of Fe-bridged ternary complexation on CA retention. In conclusion, these findings will enhance our understanding of the dynamics of CA and other LMWOAs in tropical soils.« less
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4. OPPOSITE VIEW OF PHOTO CA2163 SHOWING ORIGINAL WEST BANK ...
4. OPPOSITE VIEW OF PHOTO CA-216-3 SHOWING ORIGINAL WEST BANK FLUME PIER AT PHOTO LEFT CENTER AND NEW HIGHWAY 190 BRIDGE ABOVE FLUME. VIEW IS A 1998 DUPLICATION OF HISTORIC VIEW SHOWN IN PHOTO CA-216-11. VIEW TO NORTHWEST. - Tule River Hydroelectric Project, Water Conveyance System, Middle Fork Tule River, Springville, Tulare County, CA
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74. Photocopy of photograph (original print in the Minnesota Historical ...
74. Photocopy of photograph (original print in the Minnesota Historical Society, St. Paul, Minnesota, 1896. #35105.) FIRST ROBERT STREET BRIDGE, ca. 1896 - Smith Avenue High Bridge, Smith Avenue between Cherokee Avenue & Cliff Street, Saint Paul, Ramsey County, MN
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10. VIEW SHOWING FRAGMENTED AND INTACT ORIGINAL SIDEWALK ALONG SOUTH ...
10. VIEW SHOWING FRAGMENTED AND INTACT ORIGINAL SIDEWALK ALONG SOUTH SIDE OF SHERMAN CANAL, BETWEEN DELL AVENUE VEHICULAR BRIDGE AND GRAND CANAL COURT PEDESTRIAN BRIDGE, LOOKING EAST - Venice Canals, Community of Venice, Los Angeles, Los Angeles County, CA
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4. HISTORIC PHOTOGRAPH, VIEW OF MERRITT PARKWAY LOOKING WEST FROM ...
4. HISTORIC PHOTOGRAPH, VIEW OF MERRITT PARKWAY LOOKING WEST FROM ROUND HILL ROAD BRIDGE, CA. 1940. CONNECTICUT DEPARTMENT OF TRANSPORTATION. - Merritt Parkway, Round Hill Road Bridge, Spanning Merritt Parkway at 3.5 mile mark, Greenwich, Fairfield County, CT
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14. Toll Plaza and approach signage on 180 looking north ...
14. Toll Plaza and approach signage on 1-80 looking north (East 1-80). Photo taken from 250 feet north of 1959 bridge (east-bound 1-80). - Carquinez Bridge, Spanning Carquinez Strait at Interstate 80, Vallejo, Solano County, CA
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View of movable span and point truss (to right), from ...
View of movable span and point truss (to right), from navy land, looking west, showing bridge in context of navigational channel. - Naval Supply Annex Stockton, Daggett Road Bridge, Daggett Road traversing Burns Cut Off, Stockton, San Joaquin County, CA
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Stelzer, Julian E.; Larsson, Lars; Fitzsimons, Daniel P.; Moss, Richard L.
2006-01-01
Recent evidence suggests that ventricular ejection is partly powered by a delayed development of force, i.e., stretch activation, in regions of the ventricular wall due to stretch resulting from torsional twist of the ventricle around the apex-to-base axis. Given the potential importance of stretch activation in cardiac function, we characterized the stretch activation response and its Ca2+ dependence in murine skinned myocardium at 22°C in solutions of varying Ca2+ concentrations. Stretch activation was induced by suddenly imposing a stretch of 0.5–2.5% of initial length to the isometrically contracting muscle and then holding the muscle at the new length. The force response to stretch was multiphasic: force initially increased in proportion to the amount of stretch, reached a peak, and then declined to a minimum before redeveloping to a new steady level. This last phase of the response is the delayed force characteristic of myocardial stretch activation and is presumably due to increased attachment of cross-bridges as a consequence of stretch. The amplitude and rate of stretch activation varied with Ca2+ concentration and more specifically with the level of isometric force prior to the stretch. Since myocardial force is regulated both by Ca2+ binding to troponin-C and cross-bridge binding to thin filaments, we explored the role of cross-bridge binding in the stretch activation response using NEM-S1, a strong-binding, non-force–generating derivative of myosin subfragment 1. NEM-S1 treatment at submaximal Ca2+-activated isometric forces significantly accelerated the rate of the stretch activation response and reduced its amplitude. These data show that the rate and amplitude of myocardial stretch activation vary with the level of activation and that stretch activation involves cooperative binding of cross-bridges to the thin filament. Such a mechanism would contribute to increased systolic ejection in response to increased delivery of activator Ca2+ during excitation–contraction coupling. PMID:16446502
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NASA Astrophysics Data System (ADS)
Thomas, B. W. M.; Mead, R. N.; Mountjoy, G.
2006-05-01
Aluminate glasses are difficult to prepare as they do not contain traditional network formers, but they are promising materials for optical applications. The atomic structure of calcium aluminate glasses has been studied using several experimental techniques. The current study uses molecular dynamics to obtain a model of a (CaO)0.625(Al2O3)0.375 glass close to the eutectic. The glass consists of a tetrahedral alumina network with average network polymerization \\langle Q^{n}\\rangle of n = 3.3. Ca acts as a network modifier with average coordination of 6.2. Ca is typically coordinated to three bridging oxygens (Ob) and three non-bridging oxygens (Onb), with Ca-Onb bonds noticeably shorter than the Ca-Ob bonds. A new method of analysing modifier cation coordination is presented, which specifically shows the distribution of Ca coordination NCaO in terms of combinations of NCaOb and NCaOnb. Ob is most often coordinated to two Al plus two Ca, and Onb is most often coordinated to one Al plus three Ca. The typical coordinations of Ca, Ob, and Onb all have a noticeable similarity to those for the 5CaO·3Al2O3 crystal. The Ca-Ca distribution shows a clear similarity to that for (CaO)0.5(SiO2)0.5 glass, and this is attributed to the equal atomic number densities of Ca in these glasses.
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Diederichs, Frank
2012-01-01
ATP delivery and its usage are achieved by cycling of respective intermediates through interconnected coupled reactions. At steady state, cycling between coupled reactions always occurs at zero resistance of the whole cycle without dissipation of free energy. The cross-bridge cycle can also be described by a system of coupled reactions: one energising reaction, which energises myosin heads by coupled ATP splitting, and one de-energising reaction, which transduces free energy from myosin heads to coupled actin movement. The whole cycle of myosin heads via cross-bridge formation and dissociation proceeds at zero resistance. Dissipation of free energy from coupled reactions occurs whenever the input potential overcomes the counteracting output potential. In addition, dissipation is produced by uncoupling. This is brought about by a load dependent shortening of the cross-bridge stroke to zero, which allows isometric force generation without mechanical power output. The occurrence of maximal efficiency is caused by uncoupling. Under coupled conditions, Hill’s equation (velocity as a function of load) is fulfilled. In addition, force and shortening velocity both depend on [Ca2+]. Muscular fatigue is triggered when ATP consumption overcomes ATP delivery. As a result, the substrate of the cycle, [MgATP2−], is reduced. This leads to a switch off of cycling and ATP consumption, so that a recovery of [ATP] is possible. In this way a potentially harmful, persistent low energy state of the cell can be avoided. PMID:24957757
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2. HISTORIC PHOTOGRAPH, MERRITT PARKWAY UNDER CONSTRUCTION,LOOKING WEST TO ROUND ...
2. HISTORIC PHOTOGRAPH, MERRITT PARKWAY UNDER CONSTRUCTION,LOOKING WEST TO ROUND HILL ROAD BRIDGE, CA. 1936. COLLECTION OF GEORGE L. LARNED. - Merritt Parkway, Round Hill Road Bridge, Spanning Merritt Parkway at 3.5 mile mark, Greenwich, Fairfield County, CT
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9. Detail, typical bearing, upstream side of west end of ...
9. Detail, typical bearing, upstream side of west end of Bridge Number 301.85, view to east, 210mm lens with electronic flash fill. - Southern Pacific Railroad Shasta Route, Bridge No. 301.85, Milepost 301.85, Pollard Flat, Shasta County, CA
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49. Neg. No. 7268C, ca. 1930, PhotographerUnknown, OVERALL VIEW OF ...
49. Neg. No. 7268C, ca. 1930, Photographer-Unknown, OVERALL VIEW OF THE FORD MOTOR COMPANY ASSEMBLY PLANT FROM THE HENRY FORD BRIDGE, PRIOR TO CONSTRUCTION OF THE PRESSED STEEL BUILDING - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA
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Structural dynamics of F-actin: I. Changes in the C terminus.
Orlova, A; Egelman, E H
1995-02-03
The biochemical properties of G-actin, and the kinetics of polymerization of G-actin into F-actin, are dependent upon whether Mg2+ or Ca2+ is bound at the high-affinity metal-binding site in actin. Three-dimensional reconstructions from electron micrographs show that a bridge of density, that we interpret as arising from a major shift of the C terminus, exists between the two strands of the filament in Ca(2+)-actin that is absent in Mg(2+)-actin. This bridge is also absent in models of F-actin built from an atomic structure of G-Ca(2+)-actin. The cleavage of the DNase I-binding loop in actin between residues 42 and 43, with the non-covalent association of the 42 cleaved residues with the remainder of the actin, induces an even larger bridge of density between the two strands. When the bridge is absent, the two C-terminal residues in F-actin are easily cleaved by trypsin, while these residues become increasingly resistant to tryptic cleavage as the bridge becomes more prominent. Conversely, cleavage of the two C-terminal residues leads to a conformational change in the DNase I-binding loop. Since both the DNase I-binding loop and the metal-binding site are quite distant from the C terminus, large allosteric effects must exist in F-actin. The conformational change in F-actin that results from the creation of this bridge may be induced by myosin binding, since this movement generates changes in actin's diffraction that are very similar to the changes in the muscle X-ray pattern during activation that are associated with the binding of myosin to the thin filament.
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Yuen, Michaela; Cooper, Sandra T.; Marston, Steve B.; Nowak, Kristen J.; McNamara, Elyshia; Mokbel, Nancy; Ilkovski, Biljana; Ravenscroft, Gianina; Rendu, John; de Winter, Josine M.; Klinge, Lars; Beggs, Alan H.; North, Kathryn N.; Ottenheijm, Coen A.C.; Clarke, Nigel F.
2015-01-01
Dominant mutations in TPM3, encoding α-tropomyosinslow, cause a congenital myopathy characterized by generalized muscle weakness. Here, we used a multidisciplinary approach to investigate the mechanism of muscle dysfunction in 12 TPM3-myopathy patients. We confirm that slow myofibre hypotrophy is a diagnostic hallmark of TPM3-myopathy, and is commonly accompanied by skewing of fibre-type ratios (either slow or fast fibre predominance). Patient muscle contained normal ratios of the three tropomyosin isoforms and normal fibre-type expression of myosins and troponins. Using 2D-PAGE, we demonstrate that mutant α-tropomyosinslow was expressed, suggesting muscle dysfunction is due to a dominant-negative effect of mutant protein on muscle contraction. Molecular modelling suggested mutant α-tropomyosinslow likely impacts actin–tropomyosin interactions and, indeed, co-sedimentation assays showed reduced binding of mutant α-tropomyosinslow (R168C) to filamentous actin. Single fibre contractility studies of patient myofibres revealed marked slow myofibre specific abnormalities. At saturating [Ca2+] (pCa 4.5), patient slow fibres produced only 63% of the contractile force produced in control slow fibres and had reduced acto-myosin cross-bridge cycling kinetics. Importantly, due to reduced Ca2+-sensitivity, at sub-saturating [Ca2+] (pCa 6, levels typically released during in vivo contraction) patient slow fibres produced only 26% of the force generated by control slow fibres. Thus, weakness in TPM3-myopathy patients can be directly attributed to reduced slow fibre force at physiological [Ca2+], and impaired acto-myosin cross-bridge cycling kinetics. Fast myofibres are spared; however, they appear to be unable to compensate for slow fibre dysfunction. Abnormal Ca2+-sensitivity in TPM3-myopathy patients suggests Ca2+-sensitizing drugs may represent a useful treatment for this condition. PMID:26307083
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Yuen, Michaela; Cooper, Sandra T; Marston, Steve B; Nowak, Kristen J; McNamara, Elyshia; Mokbel, Nancy; Ilkovski, Biljana; Ravenscroft, Gianina; Rendu, John; de Winter, Josine M; Klinge, Lars; Beggs, Alan H; North, Kathryn N; Ottenheijm, Coen A C; Clarke, Nigel F
2015-11-15
Dominant mutations in TPM3, encoding α-tropomyosinslow, cause a congenital myopathy characterized by generalized muscle weakness. Here, we used a multidisciplinary approach to investigate the mechanism of muscle dysfunction in 12 TPM3-myopathy patients. We confirm that slow myofibre hypotrophy is a diagnostic hallmark of TPM3-myopathy, and is commonly accompanied by skewing of fibre-type ratios (either slow or fast fibre predominance). Patient muscle contained normal ratios of the three tropomyosin isoforms and normal fibre-type expression of myosins and troponins. Using 2D-PAGE, we demonstrate that mutant α-tropomyosinslow was expressed, suggesting muscle dysfunction is due to a dominant-negative effect of mutant protein on muscle contraction. Molecular modelling suggested mutant α-tropomyosinslow likely impacts actin-tropomyosin interactions and, indeed, co-sedimentation assays showed reduced binding of mutant α-tropomyosinslow (R168C) to filamentous actin. Single fibre contractility studies of patient myofibres revealed marked slow myofibre specific abnormalities. At saturating [Ca(2+)] (pCa 4.5), patient slow fibres produced only 63% of the contractile force produced in control slow fibres and had reduced acto-myosin cross-bridge cycling kinetics. Importantly, due to reduced Ca(2+)-sensitivity, at sub-saturating [Ca(2+)] (pCa 6, levels typically released during in vivo contraction) patient slow fibres produced only 26% of the force generated by control slow fibres. Thus, weakness in TPM3-myopathy patients can be directly attributed to reduced slow fibre force at physiological [Ca(2+)], and impaired acto-myosin cross-bridge cycling kinetics. Fast myofibres are spared; however, they appear to be unable to compensate for slow fibre dysfunction. Abnormal Ca(2+)-sensitivity in TPM3-myopathy patients suggests Ca(2+)-sensitizing drugs may represent a useful treatment for this condition. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.
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20. San FranciscoOakland Bay Bridge contract recipients, April 28, 1933, ...
20. San Francisco-Oakland Bay Bridge contract recipients, April 28, 1933, photographer unknown. Standing, left to right: Edward J. Schneider, Columbia Steel Corporation; C.C. Horton, Healy-Tibbitts Construction Company; Henry J. Kaiser, Bridge Builders, Inc.; Albert Huber, Clinton Construction Company; Allan McDonald, Transbay Construction Company; C.C. Carleton, Chief, Division of Contracts and Rights of Way, California Department of Public Works. Seated, left to right: Henry J. Brunnier, Consulting Engineer, Member of Consulting Board, San Francisco-Oakland Bay Bridge; Charles E. Andrew, Bridge Engineer, San Francisco-Oakland Bay Bridge; Earl Lee Kelly, Director, California Department of Public Works; Harrison S. Robinson, President, Financial ... - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA
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77 FR 3664 - Drawbridge Operation Regulation; Sacramento River, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2012-01-25
... drawbridge), to open on signal from May 1 through October 31 from 6 a.m. to 10 p.m. and from November 1 through April 30 from 9 a.m. to 5 p.m. At all other times, the draws shall open on signal if at least four... saving the bridge owner the cost to operate a bridge, nor to save wear and tear mechanically on a bridge...
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78 FR 68995 - Safety Zone: Vessel Removal From the Oakland Estuary, Alameda, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2013-11-18
...-AA00 Safety Zone: Vessel Removal From the Oakland Estuary, Alameda, CA AGENCY: Coast Guard, DHS. ACTION... waters of the Oakland Estuary just north of the Park Street Bridge in Alameda, CA in support of the Oakland Estuary Closure for the Vessel Removal Project on November 4, 2013 through November 22, 2013. This...
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9. Photocopy of photograph (original print at Riverside Library, Local ...
9. Photocopy of photograph (original print at Riverside Library, Local History Collection), photographer unknown, October 1916. FORMER 'VICTORIA BRIDGE' (HOWE DECK TRUSS SUPPORTED BY TRESTLE) LOOKING SOUTHWEST, SHOWING STREETCAR AND THATCH-ROOFED, CANTILEVERED PEDESTRIAN PLATFORM - Victoria Bridge, Spanning Tequesquite Arroyo, Riverside, Riverside County, CA
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403. Delineator Unknown May 2, 1933 STUDY FOR SUSPENSION TOWERS; ...
403. Delineator Unknown May 2, 1933 STUDY FOR SUSPENSION TOWERS; SAN FRANCISCO - OAKLAND BAY BRIDGE; TIMOTHY L. PFLUEGER, ARTHUR BROWN JR., JOHN J. DONOVAN; BOARD OF CONSULTING ARCHITECTS; SCHEME 2 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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Force transients and minimum cross-bridge models in muscular contraction
Halvorson, Herbert R.
2010-01-01
Two- and three-state cross-bridge models are considered and examined with respect to their ability to predict three distinct phases of the force transients that occur in response to step change in muscle fiber length. Particular attention is paid to satisfying the Le Châtelier–Brown Principle. This analysis shows that the two-state model can account for phases 1 and 2 of a force transient, but is barely adequate to account for phase 3 (delayed force) unless a stretch results in a sudden increase in the number of cross-bridges in the detached state. The three-state model (A → B → C → A) makes it possible to account for all three phases if we assume that the A → B transition is fast (corresponding to phase 2), the B → C transition is of intermediate speed (corresponding to phase 3), and the C → A transition is slow; in such a scenario, states A and C can support or generate force (high force states) but state B cannot (detached, or low-force state). This model involves at least one ratchet mechanism. In this model, force can be generated by either of two transitions: B → A or B → C. To determine which of these is the major force-generating step that consumes ATP and transduces energy, we examine the effects of ATP, ADP, and phosphate (Pi) on force transients. In doing so, we demonstrate that the fast transition (phase 2) is associated with the nucleotide-binding step, and that the intermediate-speed transition (phase 3) is associated with the Pi-release step. To account for all the effects of ligands, it is necessary to expand the three-state model into a six-state model that includes three ligand-bound states. The slowest phase of a force transient (phase 4) cannot be explained by any of the models described unless an additional mechanism is introduced. Here we suggest a role of series compliance to account for this phase, and propose a model that correlates the slowest step of the cross-bridge cycle (transition C → A) to: phase 4 of step analysis, the rate constant ktr of the quick-release and restretch experiment, and the rate constant kact for force development time course following Ca2+ activation. PMID:18425593
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79. Photocopy of photograph (original print in the Minnesota Historical ...
79. Photocopy of photograph (original print in the Minnesota Historical Society, St. Paul, Minnesota, ca. 1890, #741.) ALEXANDER RAMSEY HOUSE, ca. 1890 - Smith Avenue High Bridge, Smith Avenue between Cherokee Avenue & Cliff Street, Saint Paul, Ramsey County, MN
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71. Photocopy of photograph (original print in the Minnesota Historical ...
71. Photocopy of photograph (original print in the Minnesota Historical Society, St. Pal, Minnesota, ca. 1905. #30454.) VIEW TO NORTHWEST, POSSIBLY AFTER THE REOPENING OF THE BRIDGE IN MAY 1905 - Smith Avenue High Bridge, Smith Avenue between Cherokee Avenue & Cliff Street, Saint Paul, Ramsey County, MN
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North elevation from shoulder of Altamont Pass Road; Interstate Highway ...
North elevation from shoulder of Altamont Pass Road; Interstate Highway 5 viaduct in background; former Western Pacific (now Union Pacific) Railroad at right; abandoned Southern Pacific right of way beneath bridge; view to southwest; 90 mm lens - Carroll Overhead Bridge, Altamont Pass Road, Livermore, Alameda County, CA
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402. Delineator Unknown May 2, 1933 STUDY FOR SUSPENSION TOWERS; ...
402. Delineator Unknown May 2, 1933 STUDY FOR SUSPENSION TOWERS; SAN FRANCISCO - OAKLAND BAY BRIDGE; TIMOTHY L. PFLUEGER, ARTHUR BROWN JR., JOHN J. DONOVAN; BOARD OF CONSULTING ARCHITECTS; SCHEME 1A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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404. Delineator Unknown June 1, 1933 STUDY FOR TOP OF ...
404. Delineator Unknown June 1, 1933 STUDY FOR TOP OF SUSPENSION TOWERS; SAN FRANCISCO OAKLAND BAY BRIDGE; TIMOTHY L. PFLUEGER, ARTHUR BROWN JR., JOHN J. DONOVAN; BOARD OF CONSULTING ARCHITECTS; SCHEME 7-A - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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Nishiye, E; Somlyo, A V; Török, K; Somlyo, A P
1993-01-01
1. The effects of MgADP on cross-bridge kinetics were investigated using laser flash photolysis of caged ATP (P3-1(2-nitrophenyl) ethyladenosine 5'-triphosphate), in guinea-pig portal vein smooth muscle permeabilized with Staphylococcus aureus alpha-toxin. Isometric tension and in-phase stiffness transitions from rigor state were monitored upon photolysis of caged ATP. The estimated concentration of ATP released from caged ATP by high-pressure liquid chromatography (HPLC) was 1.3 mM. 2. The time course of relaxation initiated by photolysis of caged ATP in the absence of Ca2+ was well fitted during the initial 200 ms by two exponential functions with time constants of, respectively, tau 1 = 34 ms and tau 2 = 1.2 s and relative amplitudes of 0.14 and 0.86. Multiple exponential functions were needed to fit longer intervals; the half-time of the overall relaxation was 0.8 s. The second order rate constant for cross-bridge detachment by ATP, estimated from the rate of initial relaxation, was 0.4-2.3 x 10(4) M-1 s-1. 3. MgADP dose dependently reduced both the relative amplitude of the first component and the rate constant of the second component of relaxation. Conversely, treatment of muscles with apyrase, to deplete endogenous ADP, increased the relative amplitude of the first component. In the presence of MgADP, in-phase stiffness decreased during force maintenance, suggesting that the force per cross-bridge increased. The apparent dissociation constant (Kd) of MgADP for the cross-bridge binding site, estimated from its concentration-dependent effect on the relative amplitude of the first component, was 1.3 microM. This affinity is much higher than the previously reported values (50-300 microM for smooth muscle; 18-400 microM for skeletal muscle; 7-10 microM for cardiac muscle). It is possible that the high affinity reflects the properties of a state generated during the co-operative reattachment cycle, rather than that of the rigor bridge. 4. The rate constant of MgADP release from cross-bridges, estimated from its concentration-dependent effect on the rate constant of the second (tau 2) component, was 0.35-7.7 s-1. To the extent that reattachment of cross-bridges could slow relaxation even during the initial 200 ms, this rate constant may be an underestimate. 5. Inorganic phosphate (Pi, 30 mM) did not affect the rate of relaxation during the initial approximately 50 ms, but accelerated the slower phase of relaxation, consistent with a cyclic cross-bridge model in which Pi increases the proportion of cross-bridges in detached ('weakly bound') states.(ABSTRACT TRUNCATED AT 400 WORDS) Images Fig. 1 PMID:8487195
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30. RW Meyer Sugar Mill: 18761889. Threeroll sugar mill: oneton ...
30. RW Meyer Sugar Mill: 1876-1889. Three-roll sugar mill: one-ton daily processing capacity. Manufactured by Edwin Maw, Liverpool, England, ca. 1885-1870. View: Masonry-lined passage-way leading to the mill at the center of its circular masonry enclosure. The passageway permitted cane to be carried to the mill and cane trash (bagasse) to be carried away. Bridges over the passageways, no longer in place, permitted the mill animals to circle and power the mill from above. - R. W. Meyer Sugar Mill, State Route 47, Kualapuu, Maui County, HI
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2012-01-01
atmosphere model, Int. J . High Perform. Comput. Appl. 26 (1) (2012) 74–89. [8] J.M. Dennis, M. Levy, R.D. Nair, H.M. Tufo, T . Voran. Towards and efficient...26] A. Klockner, T . Warburton, J . Bridge, J.S, Hesthaven, Nodal discontinuous galerkin methods on graphics processors, J . Comput. Phys. 228 (21) (2009...mode James F. Kelly, Francis X. Giraldo ⇑ Department of Applied Mathematics, Naval Postgraduate School, Monterey, CA, United States a r t i c l e i n
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72. MISSISSIPPI, MONROE CO. MAP OF MONROE COUNTY, ca. 1925 ...
72. MISSISSIPPI, MONROE CO. MAP OF MONROE COUNTY, ca. 1925 Broad side of map of Monroe Co., 'Compliments of Home Mortgage & Realty Co., Amory, Miss.' Orig. scale: ca. 1 in. to 2 mi. No date. Property of Helen (Mrs. Sam L.) Crawford, Hamilton, Ms. Sarcone Photography, Columbus, Ms., Sep 1978. - Bridges of the Upper Tombigbee River Valley, Columbus, Lowndes County, MS
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5. Downstream elevation, view to southeast. Dark stains on side ...
5. Downstream elevation, view to southeast. Dark stains on side of main girder are from deck drain scuppers, marking deck level within the girders. Compare this view and CA-126-7 to CA-126-19 for indication of severity of siltation of Salt River channel has silted. - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA
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Li, Bao-Wei; Ouyang, Shun-Li; Zhang, Xue-Feng; Jia, Xiao-Lin; Deng, Lei-Bo; Liu, Fang
2014-07-01
In the present paper, nanocrystalline glass-ceramic of CaO-MgO-Al2O3-SiO2 system was produced by melting method. The CaO-MgO-Al2O3-SiO2 nanocrystalline glass-ceramic was measured by Raman spectroscopy in the temperature range from -190 to 310 degrees C in order to study the effect of temperature on the structure of this system nanocrystalline glass-ceramics. The results showed that different non-bridge oxygen bond silicon-oxygen tetrahedron structural unit changes are not consistent with rising temperature. Further analyses indicated that: the SiO4 tetrahedron with 2 non-bridged oxygen (Q2), the SiO4 tetrahedron with 3 non-bridged oxygen (Q(1)), which are situated at the edge of the 3-D SiO4 tetrahedrons network, and the SiO4 tetrahedron with 4 non-bridged oxygen (Q(0)), which is situated outside the 3-D network all suffered a significant influence by the temperature change, which has been expressed as: shifts towards the high wave-number, increased bond force constants, and shortened bond lengths. This paper studied the influence of temperature on CMAS system nanocrystalline glass-ceramics using variable temperature Raman technology. It provides experiment basis to the research on external environment influence on CMAS system nanocrystalline glass-ceramics materials in terms of structure and performance. In addition, the research provides experimental basis for controlling the expansion coefficient of nanocrystalline glass-ceramic of CaO-MgO-Al2O3-SiO2 system.
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15. STRESS SHEET. American Bridge Company, New York Office, 30 ...
15. STRESS SHEET. American Bridge Company, New York Office, 30 Church Street, sheet no. C516, dated March 12, 1928, approved March 16, 1928, order no. F5073. For U.S. Steel Products Company, Pacific Coast Depot. For Southern Pacific Company, Pacific Lines, 1st crossing, Napa River, near Napa, Western Division, customer's order no. 8873-P-28746. Various scales. - Napa River Railroad Bridge, Spanning Napa River, east of Soscol Avenue, Napa, Napa County, CA
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33 CFR 115.30 - Sufficiency of State authority for bridges.
Code of Federal Regulations, 2011 CFR
2011-07-01
... for bridges. 115.30 Section 115.30 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES BRIDGE LOCATIONS AND CLEARANCES; ADMINISTRATIVE PROCEDURES § 115.30 Sufficiency of State authority for bridges. An opinion of the attorney general of the State as to the sufficiency of State...
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33 CFR 115.30 - Sufficiency of State authority for bridges.
Code of Federal Regulations, 2010 CFR
2010-07-01
... for bridges. 115.30 Section 115.30 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES BRIDGE LOCATIONS AND CLEARANCES; ADMINISTRATIVE PROCEDURES § 115.30 Sufficiency of State authority for bridges. An opinion of the attorney general of the State as to the sufficiency of State...
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6. VIEW OF APPROACH SPAN AND MAIN SPAN OF THE ...
6. VIEW OF APPROACH SPAN AND MAIN SPAN OF THE ACCESS BRIDGE AND INTAKE PIER, LOOKING SOUTHWEST. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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9. VIEW OF INTAKE PIER AND MAIN SPAN OF ACCESS ...
9. VIEW OF INTAKE PIER AND MAIN SPAN OF ACCESS BRIDGE FROM WATER LEVEL, LOOKING NORTHWEST. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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NASA Astrophysics Data System (ADS)
Jansson, Erik V.
2003-10-01
A method to measure acoustical properties of the violin has been developed. The bridge is excited by an impulse force hammer (see www.speech.kth.se/music/acviguit4). Bridge vibrations are recorded by a small magnet and an electrical coil. Measurements can be made in an ordinary room and give a record of properties built into the violin body. An old good Polish violin, B Dankwart, Vilnius ca. 1600 shows typical results with peaks P1, P2 and the BH-hill. Our goal is as suggested by Gabriel Weinreich ``to understand, not to copy,''-Stradivarius. By shifting the soundpost position the peaks P1, P2 and BH can be somewhat monitored. It can be shown that the BH is not confined to the bridge only. The feet distance of the bridge is important but also the top plate. Marcin Groblicz, the great Polish violin maker, was court instrument maker in Krakow ca. 1600 to Sigismund, King of Poland and Sweden. I believe Gabriel Weinreich has some connection to Vilnius in Poland but unfortunately not to Sweden. We like Gabi.
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Consistency of the New York State bridge inspection program.
DOT National Transportation Integrated Search
2013-09-01
The New York State Department of Transportation (NYSDOT) maintains an inventory of over 17,000 highway : bridges across the state. As per New York States Uniform Code of Bridge Inspections, all bridges in New York : State are inspected biennially,...
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Rigor force responses of permeabilized fibres from fast and slow skeletal muscles of aged rats.
Plant, D R; Lynch, G S
2001-09-01
1. Ageing is generally associated with a decline in skeletal muscle mass and strength and a slowing of muscle contraction, factors that impact upon the quality of life for the elderly. The mechanisms underlying this age-related muscle weakness have not been fully resolved. The purpose of the present study was to determine whether the decrease in muscle force as a consequence of age could be attributed partly to a decrease in the number of cross-bridges participating during contraction. 2. Given that the rigor force is proportional to the approximate total number of interacting sites between the actin and myosin filaments, we tested the null hypothesis that the rigor force of permeabilized muscle fibres from young and old rats would not be different. 3. Permeabilized fibres from the extensor digitorum longus (fast-twitch; EDL) and soleus (predominantly slow-twitch) muscles of young (6 months of age) and old (27 months of age) male F344 rats were activated in Ca2+-buffered solutions to determine force-pCa characteristics (where pCa = -log(10)[Ca2+]) and then in solutions lacking ATP and Ca2+ to determine rigor force levels. 4. The rigor forces for EDL and soleus muscle fibres were not different between young and old rats, indicating that the approximate total number of cross-bridges that can be formed between filaments did not decline with age. We conclude that the age-related decrease in force output is more likely attributed to a decrease in the force per cross-bridge and/or decreases in the efficiency of excitation-contraction coupling.
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NASA Astrophysics Data System (ADS)
Negi, Sunita; Rana Atilgan, Ali; Atilgan, Canan
2012-12-01
Proteins are complex systems made up of many conformational sub-states which are mainly determined by the folded structure. External factors such as solvent type, temperature, pH and ionic strength play a very important role in the conformations sampled by proteins. Here we study the conformational multiplicity of calmodulin (CaM) which is a protein that plays an important role in calcium signaling pathways in the eukaryotic cells. CaM can bind to a variety of other proteins or small organic compounds, and mediates different physiological processes by activating various enzymes. Binding of calcium ions and proteins or small organic molecules to CaM induces large conformational changes that are distinct to each interacting partner. In particular, we discuss the effect of pH variation on the conformations of CaM. By using the pKa values of the charged residues as a basis to assign protonation states, the conformational changes induced in CaM by reducing the pH are studied by molecular dynamics simulations. Our current view suggests that at high pH, barrier crossing to the compact form is prevented by repulsive electrostatic interactions between the two lobes. At reduced pH, not only is barrier crossing facilitated by protonation of residues, but also conformations which are on average more compact are attained. The latter are in accordance with the fluorescence resonance energy transfer experiment results of other workers. The key events leading to the conformational change from the open to the compact conformation are (i) formation of a salt bridge between the N-lobe and the linker, stabilizing their relative motions, (ii) bending of the C-lobe towards the N-lobe, leading to a lowering of the interaction energy between the two-lobes, (iii) formation of a hydrophobic patch between the two lobes, further stabilizing the bent conformation by reducing the entropic cost of the compact form, (iv) sharing of a Ca+2 ion between the two lobes.
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Structure of liquid tricalcium aluminate
NASA Astrophysics Data System (ADS)
Drewitt, James W. E.; Barnes, Adrian C.; Jahn, Sandro; Kohn, Simon C.; Walter, Michael J.; Novikov, Alexey N.; Neuville, Daniel R.; Fischer, Henry E.; Hennet, Louis
2017-02-01
The atomic-scale structure of aerodynamically levitated and laser-heated liquid tricalcium aluminate (Ca3Al2O6 ) was measured at 2073(30) K by using the method of neutron diffraction with Ca isotope substitution (NDIS). The results enable the detailed resolution of the local coordination environment around calcium and aluminum atoms, including the direct determination of the liquid partial structure factor, SCaCa(Q ) , and partial pair distribution function, gCaCa(r ) . Molecular dynamics (MD) simulation and reverse Monte Carlo (RMC) refinement methods were employed to obtain a detailed atomistic model of the liquid structure. The composition Ca3Al2O6 lies at the CaO-rich limit of the CaO:Al2O3 glass-forming system. Our results show that, although significantly depolymerized, liquid Ca3Al2O6 is largely composed of AlO4 tetrahedra forming an infinite network with a slightly higher fraction of bridging oxygen atoms than expected for the composition. Calcium-centered polyhedra exhibit a wide distribution of four- to sevenfold coordinated sites, with higher coordinated calcium preferentially bonding to bridging oxygens. Analysis of the MD configuration reveals the presence of ˜10 % unconnected AlO4 monomers and Al2O7 dimers in the liquid. As the CaO concentration increases, the number of these isolated units increases, such that the upper value for the glass-forming composition of CaO:Al2O3 liquids could be described in terms of a percolation threshold at which the glass can no longer support the formation of an infinitely connected AlO4 network.
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Kunhi Mouvenchery, Yamuna; Jaeger, Alexander; Aquino, Adelia J. A.; Tunega, Daniel; Diehl, Dörte; Bertmer, Marko; Schaumann, Gabriele Ellen
2013-01-01
It is assumed to be common knowledge that multivalent cations cross-link soil organic matter (SOM) molecules via cation bridges (CaB). The concept has not been explicitly demonstrated in solid SOM by targeted experiments, yet. Therefore, the requirements for and characteristics of CaB remain unidentified. In this study, a combined experimental and molecular modeling approach was adopted to investigate the interaction of cations on a peat OM from physicochemical perspective. Before treatment with salt solutions of Al3+, Ca2+ or Na+, respectively, the original exchangeable cations were removed using cation exchange resin. Cation treatment was conducted at two different values of pH prior to adjusting pH to 4.1. Cation sorption is slower (>>2 h) than deprotonation of functional groups (<2 h) and was described by a Langmuir model. The maximum uptake increased with pH of cation addition and decreased with increasing cation valency. Sorption coefficients were similar for all cations and at both pH. This contradicts the general expectations for electrostatic interactions, suggesting that not only the interaction chemistry but also spatial distribution of functional groups in OM determines binding of cations in this peat. The reaction of contact angle, matrix rigidity due to water molecule bridges (WaMB) and molecular mobility of water (NMR analysis) suggested that cross-linking via CaB has low relevance in this peat. This unexpected finding is probably due to the low cation exchange capacity, resulting in low abundance of charged functionalities. Molecular modeling demonstrates that large average distances between functionalities (∼3 nm in this peat) cannot be bridged by CaB-WaMB associations. However, aging strongly increased matrix rigidity, suggesting successive increase of WaMB size to connect functionalities and thus increasing degree of cross-linking by CaB-WaMB associations. Results thus demonstrated that the physicochemical structure of OM is decisive for CaB and aging-induced structural reorganisation can enhance cross-link formation. PMID:23750256
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Kunhi Mouvenchery, Yamuna; Jaeger, Alexander; Aquino, Adelia J A; Tunega, Daniel; Diehl, Dörte; Bertmer, Marko; Schaumann, Gabriele Ellen
2013-01-01
It is assumed to be common knowledge that multivalent cations cross-link soil organic matter (SOM) molecules via cation bridges (CaB). The concept has not been explicitly demonstrated in solid SOM by targeted experiments, yet. Therefore, the requirements for and characteristics of CaB remain unidentified. In this study, a combined experimental and molecular modeling approach was adopted to investigate the interaction of cations on a peat OM from physicochemical perspective. Before treatment with salt solutions of Al(3+), Ca(2+) or Na(+), respectively, the original exchangeable cations were removed using cation exchange resin. Cation treatment was conducted at two different values of pH prior to adjusting pH to 4.1. Cation sorption is slower (>2 h) than deprotonation of functional groups (<2 h) and was described by a Langmuir model. The maximum uptake increased with pH of cation addition and decreased with increasing cation valency. Sorption coefficients were similar for all cations and at both pH. This contradicts the general expectations for electrostatic interactions, suggesting that not only the interaction chemistry but also spatial distribution of functional groups in OM determines binding of cations in this peat. The reaction of contact angle, matrix rigidity due to water molecule bridges (WaMB) and molecular mobility of water (NMR analysis) suggested that cross-linking via CaB has low relevance in this peat. This unexpected finding is probably due to the low cation exchange capacity, resulting in low abundance of charged functionalities. Molecular modeling demonstrates that large average distances between functionalities (∼3 nm in this peat) cannot be bridged by CaB-WaMB associations. However, aging strongly increased matrix rigidity, suggesting successive increase of WaMB size to connect functionalities and thus increasing degree of cross-linking by CaB-WaMB associations. Results thus demonstrated that the physicochemical structure of OM is decisive for CaB and aging-induced structural reorganisation can enhance cross-link formation.
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2006-07-01
Ms. Sarah Bridges USDA, Natural Resources Conservation Service • Mr. Lee Foster, US Army • Ms. Caroline Hall, National Park Service • Dr. John...Berryman Marine Corps Base Camp Pendleton, CA stanley.berryman@usmc.mil Sarah Bridges USDA, Natural Resources Conservation Service, DC sarah.bridges... bicentennial of the War of 1812. One of Georgia’s most successful projects was an excavation done by Southern Research for a Department of Transportation
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39. View of bridge operators controls in the control house ...
39. View of bridge operators controls in the control house facing south. Controls on the right are for the south span and on the left for the north span. The large dial indicator towards the top of the picture is a position indicator for the south span. Also present is a marine radio for talking to marine traffic and control ropes for the horn and siren. - Henry Ford Bridge, Spanning Cerritos Channel, Los Angeles-Long Beach Harbor, Los Angeles, Los Angeles County, CA
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Pulcastro, Hannah C; Awinda, Peter O; Methawasin, Mei; Granzier, Henk; Dong, Wenji; Tanner, Bertrand C W
2016-01-01
Titin is a giant protein spanning from the Z-disk to the M-band of the cardiac sarcomere. In the I-band titin acts as a molecular spring, contributing to passive mechanical characteristics of the myocardium throughout a heartbeat. RNA Binding Motif Protein 20 (RBM20) is required for normal titin splicing, and its absence or altered function leads to greater expression of a very large, more compliant N2BA titin isoform in Rbm20 homozygous mice (Rbm20 (ΔRRM) ) compared to wild-type mice (WT) that almost exclusively express the stiffer N2B titin isoform. Prior studies using Rbm20 (ΔRRM) animals have shown that increased titin compliance compromises muscle ultrastructure and attenuates the Frank-Starling relationship. Although previous computational simulations of muscle contraction suggested that increasing compliance of the sarcomere slows the rate of tension development and prolongs cross-bridge attachment, none of the reported effects of Rbm20 (ΔRRM) on myocardial function have been attributed to changes in cross-bridge cycling kinetics. To test the relationship between increased sarcomere compliance and cross-bridge kinetics, we used stochastic length-perturbation analysis in Ca(2+)-activated, skinned papillary muscle strips from Rbm20 (ΔRRM) and WT mice. We found increasing titin compliance depressed maximal tension, decreased Ca(2+)-sensitivity of the tension-pCa relationship, and slowed myosin detachment rate in myocardium from Rbm20 (ΔRRM) vs. WT mice. As sarcomere length increased from 1.9 to 2.2 μm, length-dependent activation of contraction was eliminated in the Rbm20 (ΔRRM) myocardium, even though myosin MgADP release rate decreased ~20% to prolong strong cross-bridge binding at longer sarcomere length. These data suggest that increasing N2BA expression may alter cardiac performance in a length-dependent manner, showing greater deficits in tension production and slower cross-bridge kinetics at longer sarcomere length. This study also supports the idea that passive mechanical characteristics of the myocardium influence ensemble cross-bridge behavior and maintenance of tension generation throughout the sarcomere.
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67. Photocopy of photograph (original print in the Minnesota Historical ...
67. Photocopy of photograph (original print in the Minnesota Historical Society, St. Paul Minnesota, ca. 1925. #35109. Photographer: St. Paul Daily News.) VIEW TO NORTHEAST, SHOWING SPAN NOS. 9-19, ca. 1925 - Smith Avenue High Bridge, Smith Avenue between Cherokee Avenue & Cliff Street, Saint Paul, Ramsey County, MN
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75. Photocopy of photograph (original print in the Minnesota Historical ...
75. Photocopy of photograph (original print in the Minnesota Historical Society, St. Paul, Minnesota, ca. 1870s. #22006. Photographer: Illingworth.) VIEW TO SOUTHEAST, SHOWING THE UPPER LEVEE AND BLUSS (on left) TO WHICH THE HIGH BRIDGE WAS BUILT - Smith Avenue High Bridge, Smith Avenue between Cherokee Avenue & Cliff Street, Saint Paul, Ramsey County, MN
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11. DETAIL VIEW OF APPROACH TO INTAKE PIER FROM ACCESS ...
11. DETAIL VIEW OF APPROACH TO INTAKE PIER FROM ACCESS BRIDGE, SHOWING DOOR TO INTERIOR GATE OPERATOR ROOM, LOOKING WEST. - Sacramento River Water Treatment Plant Intake Pier & Access Bridge, Spanning Sacramento River approximately 175 feet west of eastern levee on river; roughly .5 mile downstream from confluence of Sacramento & American Rivers, Sacramento, Sacramento County, CA
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78 FR 19585 - Drawbridge Operation Regulations; China Basin, San Francisco, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2013-04-02
... scheduled CycleSF, a community event. This deviation allows the bridge to remain in the closed-to-navigation... above Mean High Water in the closed-to-navigation position. The draw opens on signal if at least one... allow participants in the CycleSF to cross the bridge during the event. This temporary deviation has...
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12. Photocopy of antique postcard. (Original postcard is in the ...
12. Photocopy of antique postcard. (Original postcard is in the possession of the Venice Branch of the Los Angeles Public Library, publisher/photographer unknown.) VIEW OF PRE-EXISTING GRAND CANAL COURT PEDESTRIAN BRIDGE OVER CARROLL CANAL, LOOKING EAST TOWARD DELL AVENUE VEHICULAR BRIDGE - Venice Canals, Community of Venice, Los Angeles, Los Angeles County, CA
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Long-Lived Triplet Excited States of Bent-Shaped Pentacene Dimers by Intramolecular Singlet Fission.
Sakuma, Takao; Sakai, Hayato; Araki, Yasuyuki; Mori, Tadashi; Wada, Takehiko; Tkachenko, Nikolai V; Hasobe, Taku
2016-03-24
Intramolecular singlet fission (ISF) is a promising photophysical process to construct more efficient light energy conversion systems as one excited singlet state converts into two excited triplet states. Herein we synthesized and evaluated bent-shaped pentacene dimers as a prototype of ISF to reveal intrinsic characters of triplet states (e.g., lifetimes of triplet excited states). In this study, meta-phenylene-bridged TIPS-pentacene dimer (PcD-3Ph) and 2,2'-bipheynyl bridged TIPS-pentacene dimer (PcD-Biph) were newly synthesized as bent-shaped dimers. In the steady-state spectroscopy, absorption and emission bands of these dimers were fully characterized, suggesting the appropriate degree of electronic coupling between pentacene moieties in these dimers. In addition, the electrochemical measurements were also performed to check the electronic interaction between two pentacene moieties. Whereas the successive two oxidation peaks owing to the delocalization were observed in a directly linked-pentacene dimer (PcD) by a single bond, the cyclic voltammograms in PcD-Biph and PcD-3Ph implied the weaker interaction compared to that of p-phenylene-bridged TIPS-pentacene dimer (PcD-4Ph) and PcD. The femtosecond and nanosecond transient absorption spectra clearly revealed the slower ISF process in bent-shaped pentacene dimers (PcD-Biph and PcD-3Ph), more notably, the slower relaxation of the excited triplet states in PcD-Biph and PcD-3Ph. Namely, the quantum yields of triplet states (ΦT) by ISF approximately remain constant (ca. 180-200%) in all dimer systems, whereas the lifetimes of the triplet excited states became much longer (up to 360 ns) in PcD-Biph as compared to PcD-4Ph (15 ns). Additionally, the lifetimes of the corresponding triplet states in PcD-Biph and PcD-3Ph were sufficiently affected by solvent viscosity. In particular, the lifetimes of PcD-Biph triplet state in THF/paraffin (1.0 μs) increased up to approximately three times as compared to that in THF (360 ns), whereas those of PcD-4Ph were quite similar in both solvent.
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7. SITE OVERVIEW. PART 2 OF 6 PART PANORAMA WITH ...
7. SITE OVERVIEW. PART 2 OF 6 PART PANORAMA WITH NOS. CA-265-6 TO CA-265-11. ARROYO SECO PARKWAY AS SEEN FROM RADIO TOWER HILL. PART 2 SHOWS GRAND VIEW POINT AT LEFT REAR AND FIGUEROA VIADUCT OVERCROSSING; INTERSTATE 1-5 AT REAR CENTER; AVENUE 26 BRIDGE AT RIGHT CENTER. LOOKING 260° W. - Arroyo Seco Parkway, Los Angeles to Pasadena, Los Angeles, Los Angeles County, CA
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13. Detail, downstream side of Bridge Number 310.58, showing lower ...
13. Detail, downstream side of Bridge Number 310.58, showing lower chord connection, view to southeast, 210mm lens. The riveted portion of the bottom chord is at left, joined at the pin connection to the eyebars. The vertical intermediate post is a compression member, and is attached to one end of a floor beam that spans transversely below the bridge floor. There are paired diagonals to the left of the intermediate post, with a turnbuckled counter to rising diagonally to the right. The diagonals below the floor are bottom lateral members. - Southern Pacific Railroad Shasta Route, Bridge No. 310.58, Milepost 310.58, Sims, Shasta County, CA
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High-Resolution Laser Spectroscopy of Free Radicals in Nearly Degenerate Electronic States
NASA Astrophysics Data System (ADS)
Liu, Jinjun
2017-06-01
Rovibronic structure of molecules in orbitally degenerate electronic states including Renner-Teller (RT) and Jahn-Teller (JT) active molecules has been extensively studied. Less is known about rotational structure of polyatomic molecules in nearly degenerate states, especially those with low (e.g., C_s) symmetry that are subject to the pseudo-Jahn-Teller (pJT) effect. In the case of free radicals, the unpaired electron further complicates energy levels by inducing spin-orbit (SO) and spin-rotation (SR) splittings. Asymmetric deuteration or methyl substitution of C_{3v} free radicals such as CH_3O, CaCH_3, and CaOCH_3 lowers the molecular symmetry, lifts the vibronic degeneracy, and reduces the JT effect to the pJT effect. New spectroscopic models are required to reproduce the rovibronic structure and simulate the experimentally obtained spectra of pJT-active free radicals. It has been found that rotational and fine-structure analysis of spectra involving nearly degenerate states may aid in vibronic analysis and interpretation of effective molecular constants. Especially, SO and Coriolis interactions that couple the two states can be determined accurately from fitting the experimental spectra. Coupling between the two electronic states also affects the intensities of rotational and vibronic transitions. The study on free radicals in nearly degenerate states provides a promising avenue of research which may bridge the gap between symmetry-induced degenerate states and the Born-Oppenheimer (BO) limit of unperturbed electronic states.
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Intrinsic Tunneling in Phase Separated Manganites
NASA Astrophysics Data System (ADS)
Singh-Bhalla, G.; Selcuk, S.; Dhakal, T.; Biswas, A.; Hebard, A. F.
2009-02-01
We present evidence of direct electron tunneling across intrinsic insulating regions in submicrometer wide bridges of the phase-separated ferromagnet (La,Pr,Ca)MnO3. Upon cooling below the Curie temperature, a predominantly ferromagnetic supercooled state persists where tunneling across the intrinsic tunnel barriers (ITBs) results in metastable, temperature-independent, high-resistance plateaus over a large range of temperatures. Upon application of a magnetic field, our data reveal that the ITBs are extinguished resulting in sharp, colossal, low-field resistance drops. Our results compare well to theoretical predictions of magnetic domain walls coinciding with the intrinsic insulating phase.
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MARMION WAY AND AVENUE 66 ENTRANCE TO SOUTHBOUND LANES OF ...
MARMION WAY AND AVENUE 66 ENTRANCE TO SOUTHBOUND LANES OF ARROYO SECO PARKWAY. NOTE SMALL ISLAND AND SHORT ACCESS LANES. SEEN FROM SAME CAMERA POSITION ON DEBS PARK HILL AS CA-265-14. LOOKING 0°N - Arroyo Seco Parkway, Marmion Way Bridge, Milepost 29.28, Los Angeles, Los Angeles County, CA
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NASA Astrophysics Data System (ADS)
Tsivadze, A. Yu.; Fridman, A. Ya.; Morozova, E. M.; Sokolova, N. P.; Voloshchuk, A. M.; Bardyshev, I. I.; Gorbunov, A. M.; Novikov, A. K.; Polyakova, I. Ya.; Titova, B. N.; Yavich, A. A.
2018-02-01
Materials are produced with porous layers based on ethanolamine derivatives of PVC or compounds of active carbon with hydroxyethylcyclam derivatives of PVC with aqua complexes of chloride hydrogen cross-linked with the surface of cellulose or asbestos fabric. Their capacity for sorption with respect to hexane and benzene in the saturated vapor and liquid phases is determined. The dependences of current on voltage in a circuit are determined for bridges composed of these materials in air, and in the vapor and liquid phases of benzene and hexane between 3 M HCl solutions and 3 M HCl solutions containing 3 M CaCl2. It is established that only H+ ions migrate along the bridges between the HCl solutions, and H+ and Cl- ions were the only species that moved along the bridges between the HCl solutions containing CaCl2. The voltages at which the movement of ions starts are determined, and constants characterizing the conductivity of the layers are found. It is shown that these parameters depend on the structure of a layer, the nature of the fabric, and the medium surrounding a bridge.
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Bjørk, Alexandra; Dalhus, Bjørn; Mantzilas, Dimitrios; Eijsink, Vincent G H; Sirevåg, Reidun
2003-12-05
Malate dehydrogenase (MDH) from the moderately thermophilic bacterium Chloroflexus aurantiacus (CaMDH) is a tetrameric enzyme, while MDHs from mesophilic organisms usually are dimers. To investigate the potential contribution of the extra dimer-dimer interface in CaMDH with respect to thermal stability, we have engineered an intersubunit disulfide bridge designed to strengthen dimer-dimer interactions. The resulting mutant (T187C, containing two 187-187 disulfide bridges in the tetramer) showed a 200-fold increase in half-life at 75 degrees C and an increase of 15 deg. C in apparent melting temperature compared to the wild-type. The crystal structure of the mutant (solved at 1.75 A resolution) was essentially identical with that of the wild-type, with the exception of the added inter-dimer disulfide bridge and the loss of an aromatic intra-dimer contact. Remarkably, the mutant and the wild-type had similar temperature optima and activities at their temperature optima, thus providing a clear case of uncoupling of thermal stability and thermoactivity. The results show that tetramerization may contribute to MDH stability to an extent that depends strongly on the number of stabilizing interactions in the dimer-dimer interface.
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Cyclophilin A stabilizes the HIV-1 capsid through a novel non-canonical binding site
NASA Astrophysics Data System (ADS)
Liu, Chuang; Perilla, Juan R.; Ning, Jiying; Lu, Manman; Hou, Guangjin; Ramalho, Ruben; Himes, Benjamin A.; Zhao, Gongpu; Bedwell, Gregory J.; Byeon, In-Ja; Ahn, Jinwoo; Gronenborn, Angela M.; Prevelige, Peter E.; Rousso, Itay; Aiken, Christopher; Polenova, Tatyana; Schulten, Klaus; Zhang, Peijun
2016-03-01
The host cell factor cyclophilin A (CypA) interacts directly with the HIV-1 capsid and regulates viral infectivity. Although the crystal structure of CypA in complex with the N-terminal domain of the HIV-1 capsid protein (CA) has been known for nearly two decades, how CypA interacts with the viral capsid and modulates HIV-1 infectivity remains unclear. We determined the cryoEM structure of CypA in complex with the assembled HIV-1 capsid at 8-Å resolution. The structure exhibits a distinct CypA-binding pattern in which CypA selectively bridges the two CA hexamers along the direction of highest curvature. EM-guided all-atom molecular dynamics simulations and solid-state NMR further reveal that the CypA-binding pattern is achieved by single-CypA molecules simultaneously interacting with two CA subunits, in different hexamers, through a previously uncharacterized non-canonical interface. These results provide new insights into how CypA stabilizes the HIV-1 capsid and is recruited to facilitate HIV-1 infection.
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Kang, Fuxing; Wang, Qian; Shou, Weijun; Collins, Chris D; Gao, Yanzheng
2017-01-01
Bacterially extracellular biofilms play a critical role in relieving toxicity of fluoroquinolone antibiotic (FQA) pollutants, yet it is unclear whether antibiotic attack may be defused by a bacterial one-two punch strategy associated with metal-reinforced detoxification efficiency. Our findings help to assign functions to specific structural features of biofilms, as they strongly imply a molecularly regulated mechanism by which freely accessed alkali-earth metals in natural waters affect the cellular uptake of FQAs at the water-biofilm interface. Specifically, formation of alkali-earth-metal (Ca 2+ or Mg 2+ ) bridge between modeling ciprofloxacin and biofilms of Escherichia coli regulates the trans-biofilm transport rate of FQAs towards cells (135-nm-thick biofilm). As the addition of Ca 2+ and Mg 2+ (0-3.5 mmol/L, CIP: 1.25 μmol/L), the transport rates were reduced to 52.4% and 63.0%, respectively. Computational chemistry analysis further demonstrated a deprotonated carboxyl in the tryptophan residues of biofilms acted as a major bridge site, of which one side is a metal and the other is a metal girder jointly connected to the carboxyl and carbonyl of a FQA. The bacterial growth rate depends on the bridging energy at anchoring site, which underlines the environmental importance of metal bridge formed in biofilm matrices in bacterially antibiotic resistance. Copyright © 2016 Elsevier Ltd. All rights reserved.
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DOE Office of Scientific and Technical Information (OSTI.GOV)
Alagoz, H. S., E-mail: alagoz@ualberta.ca; Jeon, J.; Keating, S.
2016-04-14
We investigated magneto-transport properties of a compressively strained spatially confined La{sub 0.3}Pr{sub 0.4}Ca{sub 0.3}MnO{sub 3} (LPCMO) thin film micro-bridge deposited on LaAlO{sub 3}. Angular dependence of the magneto-resistance R(θ) of this bridge, where θ is the angle between the magnetic field and the current directions in the film plane, exhibits sharp positive and negative percolation jumps near T{sub MIT}. The sign and the magnitude of these jumps can be tuned using the magnetic field. Such behavior has not been observed in LPCMO micro-bridges subjected to tensile strain, indicating a correlation between the type of the lattice strain, the distribution ofmore » electronic domains, and the anisotropic magneto-resistance in spatially confined manganite systems.« less
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33. Three bolts on railing outside control house on north ...
33. Three bolts on railing outside control house on north tower, one bolt on first handrail post of the north span (bridge is in the open position). As the bridge opens or closes the single bolt on the handrail post moves past the three stationary bolts. This system is used by the bridge operator to judge speed and position of the north span as it opens or closes. Based on these bolts movement of the north span is speeded up or slowed down and the brakes applied during the opening and closing process. View facing east. - Henry Ford Bridge, Spanning Cerritos Channel, Los Angeles-Long Beach Harbor, Los Angeles, Los Angeles County, CA
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DOE Office of Scientific and Technical Information (OSTI.GOV)
Tai, Xi-Shi, E-mail: taixs@wfu.edu.cn; Wang, Xin
2017-03-15
A new Ca(II) coordination polymer, ([CaL(H{sub 2}O){sub 4}] · (H{sub 2}O){sub 4}){sub n} (L = 4-formyl-1,3-benzenedisulfonate-2-furoic acid hydrazide) has been prepared by one-pot synthesis method. And it was characterized by elemental analysis, IR and thermal analysis. The result of X-ray single-crystal diffraction analysis shows that the Ca(II) complex molecules form one-dimensional chain structure by the bridging oxygen atoms. The anti-tumor activity of L ligand and the Ca(II) coordination polymer has also been studied.
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Wang, Li; Bahadir, Anzel; Kawai, Masataka
2015-06-01
An increase in ionic strength (IS) lowers Ca(2+) activated tension in muscle fibres, however, its molecular mechanism is not well understood. In this study, we used single rabbit psoas fibres to perform sinusoidal analyses. During Ca(2+) activation, the effects of ligands (ATP, Pi, and ADP) at IS ranging 150-300 mM were studied on three rate constants to characterize elementary steps of the cross-bridge cycle. The IS effects were studied because a change in IS modifies the inter- and intra-molecular interactions, hence they may shed light on the molecular mechanisms of force generation. Both the ATP binding affinity (K1) and the ADP binding affinity (K 0) increased to 2-3x, and the Pi binding affinity (K5) decreased to 1/2, when IS was raised from 150 to 300 mM. The effect on ATP/ADP can be explained by stereospecific and hydrophobic interaction, and the effect on Pi can be explained by the electrostatic interaction with myosin. The increase in IS increased cross-bridge detachment steps (k2 and k-4), indicating that electrostatic repulsion promotes these steps. However, IS did not affect attachment steps (k-2 and k4). Consequently, the equilibrium constant of the detachment step (K2) increased by ~100%, and the force generation step (K4) decreased by ~30%. These effects together diminished the number of force-generating cross-bridges by 11%. Force/cross-bridge (T56) decreased by 26%, which correlates well with a decrease in the Debye length that limits the ionic atmosphere where ionic interactions take place. We conclude that the major effect of IS is a decrease in force/cross-bridge, but a decrease in the number of force generating cross-bridge also takes place. The stiffness during rigor induction did not change with IS, demonstrating that in-series compliance is not much affected by IS.
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Wang, Li; Bahadir, Anzel; Kawai, Masataka
2015-01-01
An increase in ionic strength (IS) lowers Ca2+ activated tension in muscle fibres, however, its molecular mechanism is not well understood. In this study, we used single rabbit psoas fibres to perform sinusoidal analyses. During Ca2+ activation, the effects of ligands (ATP, Pi, and ADP) at IS ranging 150 mM – 300 mM were studied on three rate constants to characterize elementary steps of the cross-bridge cycle. The IS effects were studied because a change in IS modifies the inter- and intra-molecular interactions, hence they may shed light on the molecular mechanisms of force generation. Both the ATP binding affinity (K1) and the ADP binding affinity (K0) increased to 2-3x, and the Pi binding affinity (K5) decreased to 1/2, when IS was raised from 150 mM to 300 mM. The effect on ATP/ADP can be explained by stereospecific and hydrophobic interaction, and the effect on Pi can be explained by the electrostatic interaction with myosin. The increase in IS increased cross-bridge detachment steps (k2 and k−4), indicating that electrostatic repulsion promotes these steps. However, IS did not affect attachment steps (k−2 and k4). Consequently, the equilibrium constant of the detachment step (K2) increased by ~100%, and the force generation step (K4) decreased by ~30%. These effects together diminished the number of force-generating cross-bridges by 11%. Force/cross-bridge (T56) decreased by 26%, which correlates well with a decrease in the Debye length that limits the ionic atmosphere where ionic interactions take place. We conclude that the major effect of IS is a decrease in force/cross-bridge, but a decrease in the number of force generating cross-bridge also takes place. The stiffness during rigor induction did not change with IS, demonstrating that in-series compliance is not much affected by IS. PMID:25836331
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Highway bridge inspection : state-of-the-practice survey
DOT National Transportation Integrated Search
2001-04-01
The congressionally mandated National Bridge Inspection program requires States to periodically inventory, inspect, and rate all highway bridges on public roads. The National Bridge Inspection Standards, implemented in 1971, prescribe minimum require...
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DOT National Transportation Integrated Search
1995-08-01
Bridge design engineers and local highway officials make bridge replacement decisions across the : United States. The Analytical Hierarchy Process was used to characterize the bridge material selection : decision of these individuals. State Departmen...
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11. DETAIL VIEW OF BRIDGE DATEPLATE AT SOUTHEAST CORNER OF ...
11. DETAIL VIEW OF BRIDGE DATEPLATE AT SOUTHEAST CORNER OF BRIDGE WHICH READS 'NORTH FORK OF WHITE RIVER, VINCENNES STEEL CORP., CONTRACTOR, ARKANSAS STATE HIGHWAY COMMISSION AND THE UNITED STATES BUREAU OF PUBLIC ROADS, 1936' - North Fork Bridge, Spans North Fork of White River at State Highway 5, Norfork, Baxter County, AR
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33 CFR 165.T11-534 - Safety zone; Bay Bridge construction, San Francisco Bay, San Francisco, CA.
Code of Federal Regulations, 2013 CFR
2013-07-01
... Francisco, CA. (a) Location. This temporary safety zone is established in the navigable waters of the San... construction, San Francisco Bay, San Francisco, CA. 165.T11-534 Section 165.T11-534 Navigation and Navigable... within a box connected by the following points: 37°49′06″ N, 122°21′17″ W; 37°49′01″ N, 122°21′12″ W; 37...
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2. SITE OVERVIEW. PART 2 OF 4 PART PANORAMA WITH ...
2. SITE OVERVIEW. PART 2 OF 4 PART PANORAMA WITH NOS. CA-265-1 TO CA-265-4. INTERCHANGE OF ARROYO SECO PARKWAY, INTERSTATE 1-5, AND FIGUEROA STREET AT CROSSINGS OF THE LOS ANGELES RIVER AS SEEN FROM GRAND VIEW POINT IN ELYSIAN PARK. VIEW 2 SHOWS LOS ANGELES RIVER AND FIGUEROA STREET BRIDGE IN FOREGROUND; INTERSTATE I-5 AT CENTER; AND ARROYO SECO PARKWAY AT RIGHT. LOOKING 30° NNE. - Arroyo Seco Parkway, Los Angeles to Pasadena, Los Angeles, Los Angeles County, CA
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2010-03-11
AFB CA 93524-7680 AFRL-RZ-ED-JA-2010-090 9. SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) Air...Force Research Laboratory (AFMC) AFRL/RZS 11. SPONSOR/MONITOR’S 5 Pollux Drive NUMBER(S) Edwards AFB CA 93524-7048 AFRL-RZ-ED-JA-2010...Strauss § Air Force Research Laboratory, Propellants Branch (AFRL/RZSP), 10 East Saturn Blvd., Edwards AFB, CA 93524-7680, Department of Chemistry and
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Designing timber bridge superstructures : a comparison of U.S. and Canadian bridge codes.
James Scott Groenier; James P. Wacker
2008-01-01
Several changes relating to timber bridges have been incorporated into the AASHTO-LRFD Bridge Design Specifications recently. In addition, the Federal Highway Administration is strongly encouraging an LRFD-based design approach for all new bridges in the United States. The Bridge Design Code in Canada was one of the first to adopt the limit states design philosophy,...
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Negroni, Jorge A.; Morotti, Stefano; Lascano, Elena C.; Gomes, Aldrin V.; Grandi, Eleonora; Puglisi, José L; Bers, Donald M.
2015-01-01
A five-state model of myofilament contraction was integrated into a well-established rabbit ventricular myocyte model of ion channels, Ca2+ transporters and kinase signaling to analyze the relative contribution of different phosphorylation targets to the overall mechanical response driven by β-adrenergic stimulation (β-AS). β-AS effect on sarcoplasmic reticulum Ca2+ handling, Ca2+, K+ and Cl− currents, and Na+/K+-ATPase properties were included based on experimental data. The inotropic effect on the myofilaments was represented as reduced myofilament Ca2+ sensitivity (XBCa) and titin stiffness, and increased cross-bridge (XB) cycling rate (XBcy). Assuming independent roles of XBCa and XBcy, the model reproduced experimental β-AS responses on action potentials and Ca2+ transient amplitude and kinetics. It also replicated the behavior of force-Ca2+, release-restretch, length-step, stiffness-frequency and force-velocity relationships, and increased force and shortening in isometric and isotonic twitch contractions. The β-AS effect was then switched off from individual targets to analyze their relative impact on contractility. Preventing β-AS effects on L-type Ca2+ channels or phospholamban limited Ca2+ transients and contractile responses in parallel, while blocking phospholemman and K+ channel (IKs) effects enhanced Ca2+ and inotropy. Removal of β-AS effects from XBCa enhanced contractile force while decreasing peak Ca2+ (due to greater Ca2+ buffering), but had less effect on shortening. Conversely, preventing β-AS effects on XBcy preserved Ca2+ transient effects, but blunted inotropy (both isometric force and especially shortening). Removal of titin effects had little impact on contraction. Finally, exclusion of β-AS from XBCa and XBcy while preserving effects on other targets resulted in preserved peak isometric force response (with slower kinetics) but nearly abolished enhanced shortening. β-AS effects on XBCa vs. XBcy have greater impact on isometric vs. isotonic contraction, respectively. PMID:25724724
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1. General view, east end and north side. View to ...
1. General view, east end and north side. View to southwest toward San Francisco-Oakland Bay Bridge, along general alignment of former Interurban Electric Railway main line. Note truncated catenary support arms on power poles; these originally carried overhead power supply catenary line for the electrically-powered interurban cars. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA
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13. 'Transverse Bracing for 1 208'101/2' C. to C. ...
13. 'Transverse Bracing for 1 - 208'-10-1/2' C. to C. End Pins S. Tr. Thro. Skew Span, 6th Crossing of Sacramento River, So. Pac. Co., The Phoenix Bridge Co., C.O. 836D, Drawing No. 7, Scale - 1' = 1', Engineer - B.M. Krohn, May 11th `01, Slaughter' - Southern Pacific Railroad Shasta Route, Bridge No. 301.85, Milepost 301.85, Pollard Flat, Shasta County, CA
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13. 'Portal and Transverse Bracing, 1 180'01/4' c. to ...
13. 'Portal and Transverse Bracing, 1 - 180'-0-1/4' c. to c. end pins S. Tr. Thro. Truss Span, 16th Crossing Sacramento River, Sacramento Division, So. Pac. Co., The Phoenix Bridge Co., C.O. #842, Draw #6, Engineer C. Scheidl, Draftsman Hermanns, Scale 1' = 1', Apr. 26, 1901.' - Southern Pacific Railroad Shasta Route, Bridge No. 324.99, Milepost 324.99, Shasta Springs, Siskiyou County, CA
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Detail of plaque beneath column on the south parapet at ...
Detail of plaque beneath column on the south parapet at the west end of the bridge. The plaque reads 1914; Mayor E.J. Drussel; Councilmen E.S. Henry, E.F. Hogan, R.P. Lamdin, C.F. Ross, J.H. Shuppert; Leonard & Day, Engineers; C.H. Gildersleeve, Builder. - First Street Bridge, Spanning Napa River at First Street between Soscol Avenue & Juarez Street, Napa, Napa County, CA
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Federal Register 2010, 2011, 2012, 2013, 2014
2011-10-28
... viewed at the following locations: City of Los Angeles Bureau of Engineering, Bridge Improvement Program... 1st Street, Los Angeles, CA 90033 The report can also be accessed through the project Web site at http://www.la6thstreetviaduct.org ; City Web site at http://eng.lacity.org/techdocs/emg/Environmental_Review...
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Federal Register 2010, 2011, 2012, 2013, 2014
2012-11-09
... Jefferson Ave, Ste 260, Temecula, CA 92590, (951) 760- 7390. Arctic Village, Raymond Tritt, First 530,000 Housing Construction...... Construction of 2 homes. Chief, P.O. Box 22069, Arctic Village, AK 99722, (907..., Chairperson, P.O. Box 529, to the Chere-ere Bridge RV Klamath, CA 95548, (707) 482-2431. Park. Sac & Fox Tribe...
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Nonlinear force-length relationship in the ADP-induced contraction of skeletal myofibrils.
Shimamoto, Yuta; Kono, Fumiaki; Suzuki, Madoka; Ishiwata, Shin'ichi
2007-12-15
The regulatory mechanism of sarcomeric activity has not been fully clarified yet because of its complex and cooperative nature, which involves both Ca(2+) and cross-bridge binding to the thin filament. To reveal the mechanism of regulation mediated by the cross-bridges, separately from the effect of Ca(2+), we investigated the force-sarcomere length (SL) relationship in rabbit skeletal myofibrils (a single myofibril or a thin bundle) at SL > 2.2 microm in the absence of Ca(2+) at various levels of activation by exogenous MgADP (4-20 mM) in the presence of 1 mM MgATP. The individual SLs were measured by phase-contrast microscopy to confirm the homogeneity of the striation pattern of sarcomeres during activation. We found that at partial activation with 4-8 mM MgADP, the developed force nonlinearly depended on the length of overlap between the thick and the thin filaments; that is, contrary to the maximal activation, the maximal active force was generated at shorter overlap. Besides, the active force became larger, whereas this nonlinearity tended to weaken, with either an increase in [MgADP] or the lateral osmotic compression of the myofilament lattice induced by the addition of a macromolecular compound, dextran T-500. The model analysis, which takes into account the [MgADP]- and the lattice-spacing-dependent probability of cross-bridge formation, was successfully applied to account for the force-SL relationship observed at partial activation. These results strongly suggest that the cross-bridge works as a cooperative activator, the function of which is highly sensitive to as little as
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20. 'Erection Plan, Renewal of Bridge 210 C over Sacramento ...
20. 'Erection Plan, Renewal of Bridge 210 C over Sacramento River near Tehama, Calif., 3 140'-0' S. T. Riveted Thru Truss Spans, 17'-9' C. to C. Trusses, 31'-0' C. To C. Chords. U.S.S. P. Co. Pacific Coast Dept., Order No. SF 604, Southern Pacific Co., Order No. 51168-P-38428, 1925 Specifications, Scale in. ft., American Bridge Co., Ambridge Plant, Dwgs. made at Ambridge No. 5 in charge of Reehl, Detailed by W.F.R., Date, Checked by L.A.E., Date 1/5/29, Fld. conn. chk. by ENN, Date 3/9/29, Order No. F5659, Sheet No. E3.' - Southern Pacific Railroad Shasta Route, Bridge No. 210.52, Milepost 210.52, Tehama, Tehama County, CA
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Li, Alison Y.; Stevens, Charles M.; Liang, Bo; Rayani, Kaveh; Little, Sean; Davis, Jonathan; Tibbits, Glen F.
2013-01-01
The Ca2+ binding properties of the FHC-associated cardiac troponin C (cTnC) mutation L29Q were examined in isolated cTnC, troponin complexes, reconstituted thin filament preparations, and skinned cardiomyocytes. While higher Ca2+ binding affinity was apparent for the L29Q mutant in isolated cTnC, this phenomenon was not observed in the cTn complex. At the level of the thin filament in the presence of phosphomimetic TnI, L29Q cTnC further reduced the Ca2+ affinity by 27% in the steady-state measurement and increased the Ca2+ dissociation rate by 20% in the kinetic studies. Molecular dynamics simulations suggest that L29Q destabilizes the conformation of cNTnC in the presence of phosphomimetic cTnI and potentially modulates the Ca2+ sensitivity due to the changes of the opening/closing equilibrium of cNTnC. In the skinned cardiomyocyte preparation, L29Q cTnC increased Ca2+ sensitivity in a highly sarcomere length (SL)-dependent manner. The well-established reduction of Ca2+ sensitivity by phosphomimetic cTnI was diminished by 68% in the presence of the mutation and it also depressed the SL-dependent increase in myofilament Ca2+ sensitivity. This might result from its modified interaction with cTnI which altered the feedback effects of cross-bridges on the L29Q cTnC-cTnI-Tm complex. This study demonstrates that the L29Q mutation alters the contractility and the functional effects of the phosphomimetic cTnI in both thin filament and single skinned cardiomyocytes and importantly that this effect is highly sarcomere length dependent. PMID:24260207
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Design aids of NU I-girders bridges.
DOT National Transportation Integrated Search
2010-05-01
Precast prestressed concrete I-Girder bridges have become the most dominant bridge system in the United States. In the early design : stages, preliminary design becomes a vital first step in designing an economical bridge. Within the state of Nebrask...
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6. BRIDGE LOOKING SOUTH FROM CROWN POINT STATE PARK SHOWING ...
6. BRIDGE LOOKING SOUTH FROM CROWN POINT STATE PARK SHOWING SURROUNDING DEVELOPMENT FROM CONSTRUCTION OF DAM - Columbia River Bridge at Grand Coulee Dam, Spanning Columbia River at State Route 155, Coulee Dam, Okanogan County, WA
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Code of Federal Regulations, 2010 CFR
2010-04-01
... 19 Customs Duties 1 2010-04-01 2010-04-01 false Equipment and materials for constructing bridges... bridges or tunnels between the United States and Canada or Mexico. (a) Admission of equipment and materials. Equipment for use in construction of bridges or tunnels between the United States and Canada or...
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Robert L. Smith; Robert J. Bush; Daniel L. Schmoldt
1995-01-01
Bridge design engineers and local highway officials make bridge replacement decisions across the United States. The Analytical Hierarchy Process was used to characterize the bridge material selection decision of these individuals. State Department of Transportation engineers, private consulting engineers, and local highway officials were personally interviewed in...
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Interfacial chemistry of zinc anodes for reinforced concrete structures
DOE Office of Scientific and Technical Information (OSTI.GOV)
Covino, B.S. Jr.; Bullard, S.J.; Cramer, S.D.
1997-12-01
Thermally-sprayed zinc anodes are used in both galvanic and impressed current cathodic protection systems for reinforced concrete structures. The Albany Research Center, in collaboration with the Oregon Department of Transportation, has been studying the effect of electrochemical aging on the bond strength of zinc anodes for bridge cathodic protection systems. Changes in anode bond strength and other anode properties can be explained by the chemistry of the zinc-concrete interface. The chemistry of the zinc-concrete interface in laboratory electrochemical aging studies is compared with that of several bridges with thermal-sprayed zinc anodes and which have been in service for 5 tomore » 10 years using both galvanic and impressed current cathodic protection systems. The bridges are the Cape Creek Bridge on the Oregon coast and the East Camino Undercrossing near Placerville, CA. Also reported are interfacial chemistry results for galvanized steel rebar from the 48 year old Longbird Bridge in Bermuda.« less
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12. 'Portal Strut for 1 208'101/2' C. to C. ...
12. 'Portal Strut for 1 - 208'-10-1/2' C. to C. End Pins S. Tr. Thro. Skew Span, 6th Crossing of Sacramento River, So. Pac. Co., The Phoenix Bridge Co., C.O. 836D, Drawing No. 12, Scale - 1' = 1', Engineer - B.M. Krohn, May 22nd `01, J.C.S.' - Southern Pacific Railroad Shasta Route, Bridge No. 301.85, Milepost 301.85, Pollard Flat, Shasta County, CA
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Development of a sustainable UHPC bridge deck for movable bridges : [research project capsule].
DOT National Transportation Integrated Search
2015-11-01
Louisiana has approximately 160 movable bridges, mostly in the southern part of the state, which : places Louisiana among states with the highest inventory of movable bridges in the nation. These : transportation arteries are important for the econom...
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Molecular dynamics simulations reveal the assembly mechanism of polysaccharides in marine aerosols.
Sun, Lu; Li, Xin; Hede, Thomas; Tu, Yaoquan; Leck, Caroline; Ågren, Hans
2014-12-21
The high Arctic marine environment has recently detected polymer gels in atmospheric aerosol particles and cloud water originating from the surface microlayer of the open leads within the pack ice area. These polysaccharide molecules are water insoluble but water solvated, highly surface-active and highly hydrated (99% water). In order to add to the understanding and to complement missing laboratory characterization of marine polymer gels we have in this work performed an atomistic study of the assembly process and interfacial properties of polysaccharides. Our study reveals a number of salient features of the microscopic process behind polysaccharide assembly into nanogels. With three- and four-repeating units the polysaccharides assemble into a cluster in 50 ns. The aggregates grow quicker by absorbing one or two polymers each time, depending on the unit length and the type of inter-bridging cation. Although both the hydrophobic and hydrophilic domains are contracted, the latter dominates distinctly upon the contraction of solvent accessible surface areas. The establishment of inter-chain hydrogen-bonds is the key to the assembly while ionic bridges can further promote aggregation. During the assembly of the more bent four-unit polymers, intra-chain hydrogen bonds are significantly diminished by Ca(2+). Meanwhile, the percentage of Ca(2+) acting as an ionic bridge is more eminent, highlighting the significance of Ca(2+) ions for longer-chain polysaccharides. The aggregates are able to enhance surface tension more in the presence of Ca(2+) than in the presence of Na(+) owing to their more compact structure. These conclusions all demonstrate that studies of the present kind provide insight into the self-assembly process and interfacial properties of marine gels. We hope this understanding will keep up the interest in the complex and the fascinating relationship between marine microbiology, atmospheric aerosols, clouds and climate.
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Hellman, Maarit; Piirainen, Henni; Jaakola, Veli-Pekka; Permi, Perttu
2014-01-01
NMR spectroscopy is by far the most versatile and information rich technique to study intrinsically disordered proteins (IDPs). While NMR is able to offer residue level information on structure and dynamics, assignment of chemical shift resonances in IDPs is not a straightforward process. Consequently, numerous pulse sequences and assignment protocols have been developed during past several years, targeted especially for the assignment of IDPs, including experiments that employ H(N), H(α) or (13)C detection combined with two to six indirectly detected dimensions. Here we propose two new HN-detection based pulse sequences, (HCA)CON(CAN)H and (HCA)N(CA)CO(N)H, that provide correlations with (1)H(N)(i - 1), (13)C'(i - 1) and (15)N(i), and (1)H(N)(i + 1), (13)C'(i) and (15)N(i) frequencies, respectively. Most importantly, they offer sequential links across the proline bridges and enable filling the single proline gaps during the assignment. We show that the novel experiments can efficiently complement the information available from existing HNCO and intraresidual i(HCA)CO(CA)NH pulse sequences and their concomitant usage enabled >95 % assignment of backbone resonances in cytoplasmic tail of adenosine receptor A2A in comparison to 73 % complete assignment using the HNCO/i(HCA)CO(CA)NH data alone.
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NASA Astrophysics Data System (ADS)
Morzyk-Ociepa, Barbara; Szmigiel, Ksenia; Dysz, Karolina; Turowska-Tyrk, Ilona; Michalska, Danuta
2016-11-01
Two new complexes of Cd(II) with an O-deprotonated anion of 5-methoxyindole-2-carboxylic acid (5-MeOI2CA), of the formulas [Cd(5-MeOI2CA)2(H2O)2]n (1) and [Cd3(5-MeOI2CA)6(H2O)4(DMSO)4]ṡ2DMSO (2) were synthesized. In the polymeric complex 1, the 5-MeOI2CA anion acts as a bidentate bridging ligand and the coordination environment around the Cd(II) ion can be described as a distorted octahedron. Single crystal X-ray diffraction analysis of 2 has revealed that this complex is a trimer and it crystallizes in the monoclinic system (space group P21/c with a = 20.3403(4), b = 14.3079(2), c = 15.0603(3) Å, β = 92.4341(17)°, V = 4379.00(14) Å3 and Z = 2). In 2, the 5-MeOI2CA anions act as bidentate bridging and bidentate chelating ligands. The asymmetric unit of 2 contains two crystallographically independent Cd(II) cations. One of the cations is coordinated to six oxygen atoms and shows an octahedral geometry with a rhombic deformation. The other Cd(II) cation adopts a distorted seven-coordinate pentagonal-bipyramidal geometry involving seven oxygen atoms. In 2, the DMSO solvent molecules play a key role in the formation of metal-organic frameworks by filling voids, which are created by the bridging and chelating 5-MeOI2CA anions, the cadmium cations and the other DMSO molecules coordinated to cadmium. Comprehensive theoretical calculations (including the optimized structural parameters, harmonic frequencies and vibrational intensities) were performed for 2 using the B3LYP method with the 6-311++G(d,p)/LanL2DZ basis sets. The infrared and Ramana spectra were measured and a detailed assignment of the experimental spectra of 2 was performed. All cadmium-oxygen stretching vibrations occur in the range below 400 cm-1.
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Hoshino, Takahiro; Isobe, Rina; Kaneko, Takuya; Matsuki, Yusuke; Nomiya, Kenji
2017-08-21
A novel compound containing a hexacalcium cluster cation, one carbonate anion, and one calcium cation assembled on a trimeric trititanium(IV)-substituted Wells-Dawson polyoxometalate (POM), [{Ca 6 (CO 3 )(μ 3 -OH)(OH 2 ) 18 }(P 2 W 15 Ti 3 O 61 ) 3 Ca(OH 2 ) 3 ] 19- (Ca 7 Ti 9 Trimer), was obtained as the Na 7 Ca 6 salt (NaCa-Ca 7 Ti 9 Trimer) by the reaction of calcium chloride with the monomeric trititanium(IV)-substituted Wells-Dawson POM species "[P 2 W 15 Ti 3 O 59 (OH) 3 ] 9- " (Ti 3 Monomer). Ti 3 Monomer was generated in situ under basic conditions from the separately prepared tetrameric species with bridging Ti(OH 2 ) 3 groups and an encapsulated Cl - ion, [{P 2 W 15 Ti 3 O 59 (OH) 3 } 4 {μ 3 -Ti(H 2 O) 3 } 4 Cl] 21- (Ti 16 Tetramer). The Na 7 Ca 6 salt of Ca 7 Ti 9 Trimer was characterized by complete elemental analysis, thermogravimetric (TG) and differential thermal analyses (DTA), FTIR, single-crystal X-ray structure analysis, and solution 183 W and 31 P NMR spectroscopy. X-ray crystallography revealed that the [Ca 6 (CO 3 )(μ 3 -OH)(OH 2 ) 18 ] 9+ cluster cation was composed of six calcium cations linked by one μ 6 -carbonato anion and one μ 3 -OH - anion. The cluster cation was assembled, together with one calcium ion, on a trimeric species composed of three tri-Ti(IV)-substituted Wells-Dawson subunits linked by Ti-O-Ti bonds. Ca 7 Ti 9 Trimer is an unprecedented POM species containing an alkaline-earth-metal cluster cation and is the first example of alkaline-earth-metal ions clustered around a titanium(IV)-substituted POM.
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Gomez-Mendoza, M; Marin, M Luisa; Miranda, Miguel A
2014-11-14
The aim of the present work is to develop two-channel emitters to probe local hydrophobicity by means of fluorescence quenching within different biomimetic supramolecular environments. To achieve this goal, the dansyl (Dns) and tryptophan (Trp) fluorophores have been covalently attached to cholic acid (CA) in order to ensure simultaneous incorporation of the two emitting units into the same compartment. In principle, the two fluorophores of the synthesized Dns-CA-Trp probes could either exhibit an orthogonal behavior or display excited state interactions. The fluorescence spectra of 3β-Dns-CA-Trp showed a residual Trp emission band at ca. 350 nm and an enhanced Dns maximum in the 500-550 nm region. This reveals a partial intramolecular energy transfer, which is consistent with the Dns and Trp singlet energies. Thus, the two photoactive units are not orthogonal; nevertheless, 3β-Dns-CA-Trp seems appropriate as a two-channel reporter for the supramolecular systems of interest. Fluorescence quenching of 3β-Dns-CA-Trp by iodide (which remains essentially in bulk water) was examined within sodium cholate, sodium taurocholate, sodium deoxycholate and mixed micelles. Interestingly, a decrease in the emission intensity of the two bands was observed with increasing iodide concentrations. The most remarkable effect was observed for mixed micelles, where the quenching rate constants were one order of magnitude lower than in solution. As anticipated, the quenching efficiency by iodide decreased with increasing hydrophobicity of the microenvironment, a trend that can be correlated with the relative accessibility of the probe to the ionic quencher.
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State and county delivered bridge replacement project analysis : phase I and phase II.
DOT National Transportation Integrated Search
2010-02-01
The purpose of the study was to compare the cost of state delivered bridge projects and county delivered bridge projects. A total of 190 different bridge replacement projects were analyzed. The first phase of the project focused on whether or not the...
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Bridge deficiency metric refinement : longer-term planning for state and locally-owned bridges.
DOT National Transportation Integrated Search
2010-06-01
The focus of this study was to devise a prioritization scheme for locally-owned bridges and to extend the planning time horizon for state-owned bridges. The inherent nature of the local and county structures prevents a direct application of the formu...
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Dantzig, J A; Hibberd, M G; Trentham, D R; Goldman, Y E
1991-01-01
1. The interaction between MgADP and rigor cross-bridges in glycerol-extracted single fibres from rabbit psoas muscle has been investigated using laser pulse photolysis of caged ATP (P3-1(2-nitrophenyl)ethyladenosine 5'-triphosphate) in the presence of MgADP and following small length changes applied to the rigor fibre. 2. Addition of 465 microM-MgADP to a rigor fibre caused rigor tension to decrease by 15.3 +/- 0.7% (S.E.M., n = 24 trials in thirteen fibres). The half-saturation value for this tension reduction was 18 +/- 4 microM (n = 23, thirteen fibres). 3. Relaxation from rigor by photolysis of caged ATP in the absence of Ca2+ was markedly slowed by inclusion of 20 microM-2 mM-MgADP in the photolysis medium. 4. Four phases of tension relaxation occurred with MgADP in the medium: at, a quick partial relaxation (in pre-stretch fibres); bt, a slowing of relaxation or a rise in tension for 50-100 ms; ct, a sudden acceleration of relaxation; and dt, a final, nearly exponential relaxation. 5. Experiments at varied MgATP and MgADP concentrations suggested that phase at is due to MgATP binding to nucleotide-free cross-bridges. 6. Phase bt was abbreviated by including 1-20 mM-orthophosphate (Pi) in the photolysis medium, or by applying quick stretches before photolysis or during phase bt. These results suggest that phases bt and ct are complex processes involving ADP dissociation, cross-bridge reattachment and co-operative detachment involving filament sliding and the Ca(2+)-regulatory system. 7. Stretching relaxed muscle fibres to 3.2-3.4 microns striation spacing followed by ATP removal and release of the rigor fibre until tension fell below the relaxed level allowed investigation of the strain dependence of relaxation in the regions of negative cross-bridge strain. In the presence of 50 microM-2 mM-MgADP and either 10 mM-Pi or 20 mM-2,3-butanedione monoxime, relaxation following photolysis of caged ATP was 6- to 8-fold faster for negatively strained cross-bridges than for positively strained ones. This marked strain dependence of cross-bridge detachment is predicted from the model of A. F. Huxley (1957). 8. In the presence of Ca2+, activation of contraction following photolysis of caged ATP was slowed by inclusion of 20-500 microM-MgADP in the medium. An initial decrease in tension related to cross-bridge detachment by MgATP was markedly suppressed in the presence of MgADP. 9. Ten millimolar Pi partly suppressed active tension generation in the presence of MgADP.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:1886072
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Gehlert, Sebastian; Bloch, Wilhelm; Suhr, Frank
2015-01-01
Calcium (Ca2+) plays a pivotal role in almost all cellular processes and ensures the functionality of an organism. In skeletal muscle fibers, Ca2+ is critically involved in the innervation of skeletal muscle fibers that results in the exertion of an action potential along the muscle fiber membrane, the prerequisite for skeletal muscle contraction. Furthermore and among others, Ca2+ regulates also intracellular processes, such as myosin-actin cross bridging, protein synthesis, protein degradation and fiber type shifting by the control of Ca2+-sensitive proteases and transcription factors, as well as mitochondrial adaptations, plasticity and respiration. These data highlight the overwhelming significance of Ca2+ ions for the integrity of skeletal muscle tissue. In this review, we address the major functions of Ca2+ ions in adult muscle but also highlight recent findings of critical Ca2+-dependent mechanisms essential for skeletal muscle-regulation and maintenance. PMID:25569087
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3. THREE HISTORIC PHOTOGRAPHS, VIEWS OF SCAFFOLDING AND REINFORCING BARS, ...
3. THREE HISTORIC PHOTOGRAPHS, VIEWS OF SCAFFOLDING AND REINFORCING BARS, CA. 1935. COLLECTION CONNECTICUT DEPARTMENT OF TRANSPORTATION. - Merritt Parkway, Guinea Road Bridge, Spanning Merritt Parkway, Stamford, Fairfield County, CT
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76 FR 10593 - Ocean Transportation Intermediary License Applicants
Federal Register 2010, 2011, 2012, 2013, 2014
2011-02-25
..., (Qualifying Individual), Application Type: New NVO License. Gulf South Forest Products, Inc. (NVO), 3038 N... World Bridge Line dba Green Shipping, Inc. (NVO), 16012 S. Western Avenue, 302, Gardena, CA 90247...
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DOT National Transportation Integrated Search
2009-11-01
The Oregon Department of Transportation and Portland State University evaluated the seismic : vulnerability of state highway bridges in western Oregon. The study used a computer program : called REDARS2 that simulated the damage to bridges within a t...
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DOT National Transportation Integrated Search
2014-07-01
The purpose of this report is to document Accelerated Bridge Construction (ABC) techniques on IBRD : (Innovative Bridge Research and Development) project 102470 for the construction of Bridge N-16-Q : on State Highway 69 over Turkey Creek. The constr...
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DOT National Transportation Integrated Search
2010-02-01
The purpose of the study was to compare the cost of state delivered bridge projects and county delivered bridge projects. A total of 190 different bridge replacement projects were analyzed. The first phase of the project focused on whether or not the...
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Field performance of stress-laminated timber bridges on low-volume roads
M. A. Ritter; J. P. Wacker; S. R. Duwadi
1995-01-01
Stress-laminated timber bridges were first introduced in the United States in the late 1980s. Since that time, the concept of stress-laminating has received a great deal of attention and hundreds of bridges have been built. Most of these bridges are located on rural low-volume roads. To evaluate the performance of stress-laminated bridges, the United States Department...
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Guardrails for use on historic bridges: volume 2--bridge deck overhang design.
DOT National Transportation Integrated Search
2016-11-01
Bridges that are designated historic present a special challenge to bridge engineers whenever rehabilitation work or improvements are : made to the bridges. Federal and state laws protect historically significant bridges, and railings on these bridge...
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12. FLOOR BEAMS, BRACKETS, STRINGERS. (Also includes a schedule of ...
12. FLOOR BEAMS, BRACKETS, STRINGERS. (Also includes a schedule of parts.) American Bridge Company, Ambridge Plant No. 5, sheet no. 2, dated April 2, 1928 (revised 4-24-28), order no. F5073. For U.S. Steel Products Company, Pacific Coast Depot, order no. SF578. For Southern Pacific Company, order no. 8873-P-28746. Scale 1/4 inch to one foot. - Napa River Railroad Bridge, Spanning Napa River, east of Soscol Avenue, Napa, Napa County, CA
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18. 'Erection Plan for 3 180'61/2' End Pins S. ...
18. 'Erection Plan for 3 - 180'-6-1/2' End Pins S. Track Thro. Spans, 10th, 11th & 13th Crossings of Sacramento River, Pacific Systems, Southern Pacific Co. Phoenix Bridge Co., C. O. #839, Drawing #9, Scale = 1/8' & 1' = 1 ft., Eng'r-Chas. Scheidl, Drafts. H. O. McG., April 16th, 1901.' - Southern Pacific Railroad Shasta Route, Bridge No. 310.58, Milepost 310.58, Sims, Shasta County, CA
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12. 'Erection Plan, 1 180'01/4' c. to c. End ...
12. 'Erection Plan, 1 - 180'-0-1/4' c. to c. End Pins Sing. Tr. Thro' Span, 16th Crossing over Sacramento River, Pacific System, Southern Pacific Co., Phoenix Bridge Co., C.O. #842, Drawing #13, Scale 1/8' & 1' = 1'-0', Eng'r C. Scheidl, Draftsman D. Sharp, Scale 1' = 1'-0', May 1st 1901.' - Southern Pacific Railroad Shasta Route, Bridge No. 324.99, Milepost 324.99, Shasta Springs, Siskiyou County, CA
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11. 'Erection Plan, 1 208'101/2' C. to C. End ...
11. 'Erection Plan, 1 - 208'-10-1/2' C. to C. End Pins S. Tr. Thro. Skew Span, 6th Crossing Sacramento River, Pacific System, Southern Pacific Company, The Phoenix Bridge Co., C.O. 836D, Drawing No. 13, Scale 1/8' = 1'0', Engineer, B.M. Krohn, Draftsman, W.L. Clegg, Date, May 25th 1901' - Southern Pacific Railroad Shasta Route, Bridge No. 301.85, Milepost 301.85, Pollard Flat, Shasta County, CA
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Detail of plate and gears on center pivot. From civilian ...
Detail of plate and gears on center pivot. From civilian land. Looking north. - Naval Supply Annex Stockton, Daggett Road Bridge, Daggett Road traversing Burns Cut Off, Stockton, San Joaquin County, CA
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Ko, Michelle; Murphy, Julia; Bindman, Andrew B
2015-11-01
Policymakers have increasingly promoted health services integration to improve quality and efficiency. The US health care safety net, which comprises providers of health care to uninsured, Medicaid, and other vulnerable patients, remains a largely fragmented collection of providers. We interviewed leadership from safety net hospitals and community health centers in 5 US cities (Boston, MA; Denver, CO; Los Angeles, CA; Minneapolis, MN; and San Francisco, CA) throughout 2013 on their experiences with service integration. We identify conflicts in organizational mission, identity, and consumer orientation that have fostered reluctance to enter into collaborative arrangements. We describe how smaller scale initiatives, such as capitated model for targeted populations, health information exchange, and quality improvements led by health plans, can help bridge cultural differences to lay the groundwork for developing integrated care programs.
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Heat production during contraction in skeletal muscle of hypothyroid mice
DOE Office of Scientific and Technical Information (OSTI.GOV)
Leijendekker, W.J.; van Hardeveld, C.; Elzinga, G.
1987-08-01
The effect of hypothyroidism on tension-independent and -dependent heat produced during a twitch and a tetanic contraction of extensor digitorum longus (EDL) and soleus muscle of mice was examined. The amount of heat produced during a twitch and the rate of heat development during a tetanus of EDL and soleus were measured at and above optimal length. The effect of hypothyroidism on force production was <30%. Straight lines were used to fit the relation between heat production and force. Hypothyroidism significantly decreases tension-independent heat during contraction of EDL and soleus muscle. Because the tension-independent heat is considered to be relatedmore » to the Ca{sup 2+} cycling, these findings suggest that ATP splitting due to the Ca{sup 2+} cycling is reduced in hypothyroid mice. This conclusion was strengthened by the observation that the oxalate-supported {sup 45}Ca{sup 2+}-uptake activity and {sup 45}Ca{sup 2+}-loading capacity of muscle homogenates from hypothyroid mice were reduced, respectively, to 51 and to 65% in soleus and to 63 and 73% in EDL muscle as compared with euthyroid mice. The tension-dependent rate of heat development during a tetanus was also decreased in soleus muscle of hypothyroid mice. This suggests a lower rate of ATP hydrolysis related to cross-bridge cycling in this muscle due to the hypothyroid state.« less
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Energetics of muscle contraction: further trials.
Yamada, Kazuhiro
2017-01-01
Knowledge accumulated in the field of energetics of muscle contraction has been reviewed in this article. Active muscle converts chemical energy into heat and work. Therefore, measurements of heat production and mechanical work provide the framework for understanding the process of energy conversion in contraction. In the 1970s, precise comparison between energy output and the associated chemical reactions was performed. It has been found that the two do not match in several situations, resulting in an energy balance discrepancy. More recently, efforts in resolving these discrepancies in the energy balance have been made involving chemical analysis, phosphorus nuclear magnetic resonance spectroscopy, and microcalorimetry. Through reviewing the evidence from these studies, the energy balance discrepancy developed early during isometric contraction has become well understood on a quantitative basis. In this situation energy balance is established when we take into account the binding of Ca to sarcoplasmic proteins such as troponin and parvalbumin, and also the shift of cross-bridge states. On the other hand, the energy balance discrepancy observed during rapid shortening still remains to be clarified. The problem may be related to the essential mechanism of cross-bridge action.
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1. VIEW EAST, LOOKING TOWARDS BRIDGE FROM WILSON SHUTE ROAD ...
1. VIEW EAST, LOOKING TOWARDS BRIDGE FROM WILSON SHUTE ROAD (STATE ROAD 2008) - Wilson Shute Bridge, Spanning French Creek at State Road 2008 (formerly Legislative Route 20027), Meadville, Crawford County, PA
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Spatial nonuniformity of contraction causes arrhythmogenic Ca2+ waves in rat cardiac muscle.
Ter Keurs, Henk E D J; Wakayama, Yuji; Miura, Masahito; Stuyvers, Bruno D; Boyden, Penelope A; Landesberg, Amir
2005-06-01
Landesberg and Sideman's four state model of the cardiac cross-bridge (XB) hypothesizes a feedback of force development to Ca(2+) binding by troponin C (TnC). We have further modeled this behavior and observed that the force (F)-Ca(2+) relationship as well as the F-sarcomere length (SL) relationship and the time course of F and Ca(2+) transients in cardiac muscle can be reproduced faithfully by a single effect of F on deformation of the TnC-Ca complex and, thereby, on the dissociation rate of Ca(2+). Furthermore, this feedback predicts that rapid decline of F in the activated sarcomere causes release of Ca(2+) from TnC-Ca(2+), which is sufficient to initiate arrhythmogenic Ca(2+) release from the sarcoplasmic reticulum (SR). This work investigated the initiation of Ca(2+) waves underlying triggered propagated contractions (TPCs) in rat cardiac trabeculae under conditions that simulate functional nonuniformity caused by mechanical or ischemic local damage of the myocardium. A mechanical discontinuity along the trabeculae was created by exposing the preparation to a small constant flow jet of solution that reduces excitation-contraction coupling in myocytes within that segment. Force was measured, and SL as well as [Ca(2+)](i) were measured regionally. When the jet contained caffeine, 2,3-butanedione monoxime or low-[Ca(2+)], muscle-twitch F decreased and the sarcomeres in the exposed segment were stretched by shortening the normal regions outside the jet. During relaxation, the sarcomeres in the exposed segment shortened rapidly. Short trains of stimulation at 2.5 Hz reproducibly caused Ca(2+) waves to rise from the borders exposed to the jet. Ca(2+) waves started during F relaxation of the last stimulated twitch and propagated into segments both inside and outside of the jet. Arrhythmias, in the form of nondriven rhythmic activity, were triggered when the amplitude of the Ca(2+) wave increased by raising [Ca(2+)](o). The arrhythmias disappeared when the muscle uniformity was restored by turning the jet off. These results show that nonuniform contraction can cause Ca(2+) waves underlying TPCs, and suggest that Ca(2+) dissociated from myofilaments plays an important role in the initiation of arrhythmogenic Ca(2+) waves.
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23 CFR 661.49 - Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges?
Code of Federal Regulations, 2011 CFR
2011-04-01
..., and Toll Road IRR bridges? 661.49 Section 661.49 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.49 Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? Yes. Interstate...
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23 CFR 661.49 - Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges?
Code of Federal Regulations, 2010 CFR
2010-04-01
..., and Toll Road IRR bridges? 661.49 Section 661.49 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.49 Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? Yes. Interstate...
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Coordination of XeF2 to calcium and cadmium hexafluorophosphates(V).
Bunic, Tina; Tavcar, Gasper; Tramsek, Melita; Zemva, Boris
2006-02-06
[M(XeF2)5](PF6)2 (M = Ca, Cd) complexes were prepared by the reaction of MF2 and XeF2 under pressure of gaseous PF5 in anhydrous HF as solvent. The coordination sphere of the Ca atom consists of nine fluorine atoms: three from two PF6(-) units (one bidentate and one monodentate) and one from each of six XeF2 molecules. The coordination sphere of the Cd atom consists of eight fluorine atoms: one from each of two PF6(-) units and one from each of six XeF2 molecules. Two of the XeF2 ligands about M in each compound are bridging ligands and are each linked to two M, generating infinite (-M-F-Xe-F-M-F-Xe-F-) chains along the b-axis in the Ca salt and along the c-axis in the Cd compound. The Cd2+ cation is smaller and more electronegative than the Ca2+ cation. These differences account for the higher F ligand coordination in the Ca2+ salt and for other structural features that distinguish them. The different stoichiometry of the PF6(-) salts when compared with their AsF6(-) analogues, which have the composition [M(XeF2)4](AsF6)2 (M = Ca, Cd), is in accord with the lower F ligand charge in the AsF6(-) when compared with that in the PF6(-) compound. Indeed, the AsF6(-) ligand charges appear to be similar to those in the XeF2-bridged species.
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Guardrails for use on historic bridges : volume 1--replacement strategies.
DOT National Transportation Integrated Search
2016-11-01
Bridges that are designated historic present a special challenge to bridge engineers whenever rehabilitation work or improvements are : made to the bridges. Federal and state laws protect historically significant bridges, and railings on these bridge...
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Holmes, Robert R.; Dunn, Chad J.
1996-01-01
A simplified method to estimate total-streambed scour was developed for application to bridges in the State of Illinois. Scour envelope curves, developed as empirical relations between calculated total scour and bridge-site chracteristics for 213 State highway bridges in Illinois, are used in the method to estimate the 500-year flood scour. These 213 bridges, geographically distributed throughout Illinois, had been previously evaluated for streambed scour with the application of conventional hydraulic and scour-analysis methods recommended by the Federal Highway Administration. The bridge characteristics necessary for application of the simplified bridge scour-analysis method can be obtained from an office review of bridge plans, examination of topographic maps, and reconnaissance-level site inspection. The estimates computed with the simplified method generally resulted in a larger value of 500-year flood total-streambed scour than with the more detailed conventional method. The simplified method was successfully verified with a separate data set of 106 State highway bridges, which are geographically distributed throughout Illinois, and 15 county highway bridges.
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An elevated view of the bridge deck looking east toward ...
An elevated view of the bridge deck looking east toward Battelle Laboratories and the Ohio State University. Picture taken from State Route 315. - King Avenue Bridge, Spanning Olentangy River, Columbus, Franklin County, OH
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14. Detail, typical approach span fixed bearing atop stone masonry ...
14. Detail, typical approach span fixed bearing atop stone masonry pier, view to northwest, 210mm lens. - Southern Pacific Railroad Shasta Route, Bridge No. 210.52, Milepost 210.52, Tehama, Tehama County, CA
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Center pivot, showing substantial beams that support the trusses. Looking ...
Center pivot, showing substantial beams that support the trusses. Looking north from civilian land. - Naval Supply Annex Stockton, Daggett Road Bridge, Daggett Road traversing Burns Cut Off, Stockton, San Joaquin County, CA
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Liang, Zhili; Mohanty, Paritosh; Fei, Yingwei; Landskron, Kai
2010-12-14
Coesite nanocrystals have been synthesized from periodic mesoporous organosilica (PMO) with (CH(2))(2) bridges heated at 300 °C for 150 min and 12 GPa. The crystals are not sintered, single crystalline, and have diameters of ca. 100-300 nm. Below 300 °C, an amorphous non-porous organosilica glass was obtained. Heating above 300 °C at 12 GPa results in the rapid crystal growth and micron size coesite crystals were formed.
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11. GIRDER PARTIAL ELEVATION AND SECTIONS, 80 FOOT THROUGH PLATE ...
11. GIRDER PARTIAL ELEVATION AND SECTIONS, 80 FOOT THROUGH PLATE GIRDER SPAN. (Also includes a Marking Diagram and a schedule of parts.) American Bridge Company, Ambridge Plant No. 5, sheet no. 1, dated April 7, 1928, order no. F5073. For U.S. Steel Products Company, Pacific Coast Depot, order no. SF578. For Southern Pacific Company, order no. 8873-P-28746. Scale 1/4 inch to one foot. - Napa River Railroad Bridge, Spanning Napa River, east of Soscol Avenue, Napa, Napa County, CA
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14. UPPER SHOES, FIXED SHOES, ROLLER SHOES, CENTER WEB, AND ...
14. UPPER SHOES, FIXED SHOES, ROLLER SHOES, CENTER WEB, AND ROLLER BED PLATES. (Also includes a sheet index and a schedule of parts). American Bridge Company, Ambridge Plant No. 5, sheet no. 4, dated April 7, 1928, order no. F5073. For U.S. Steel Products Company, Pacific Coast Depot, order no. SF578. For Southern Pacific Company, order no. 8873-P-28746. various scales. - Napa River Railroad Bridge, Spanning Napa River, east of Soscol Avenue, Napa, Napa County, CA
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17. 'Southern Pacific Company Pacific System, 3 180'61/2' ...
17. 'Southern Pacific Company - Pacific System, 3 - 180'-6-1/2' c. to c. end pins S. Tr. Thro. Spans, 10th, 11th and 13th Crossings of Sacramento River, also for 1 - 180'-6-1/2' c. to c. end pins S. Tr. Thro. Span, 8th Crossing Sacramento River, The Phoenixville Bridge Co., Phoenixville Pa., Apr. 9th, 1901.' - Southern Pacific Railroad Shasta Route, Bridge No. 310.58, Milepost 310.58, Sims, Shasta County, CA
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20. 'Portals and Gusset Plates for 3 180'61/2' c. ...
20. 'Portals and Gusset Plates for 3 - 180'-6-1/2' c. to c. End Pins Single Track Through Spans, 10th, 11th, & 13th Crossings of Sacramento River, Southern Pacific Co. Sacramento Division, The Phoenix Bridge Co., C.O's. 839, 840 & 841, Drawing #12, Engineer C. Scheidl, Draftsman B. Heald, Scale 1-1/2' = 1'0', April 16th, 1901.' - Southern Pacific Railroad Shasta Route, Bridge No. 310.58, Milepost 310.58, Sims, Shasta County, CA
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Partially entangled states bridge in quantum teleportation
NASA Astrophysics Data System (ADS)
Cai, Xiao-Fei; Yu, Xu-Tao; Shi, Li-Hui; Zhang, Zai-Chen
2014-10-01
The traditional method for information transfer in a quantum communication system using partially entangled state resource is quantum distillation or direct teleportation. In order to reduce the waiting time cost in hop-by-hop transmission and execute independently in each node, we propose a quantum bridging method with partially entangled states to teleport quantum states from source node to destination node. We also prove that the designed specific quantum bridging circuit is feasible for partially entangled states teleportation across multiple intermediate nodes. Compared to two traditional ways, our partially entanglement quantum bridging method uses simpler logic gates, has better security, and can be used in less quantum resource situation.
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Live-load performance evaluation of historic covered timber bridges in the United States
Junwon Seo; Travis K. Hosteng; Brent M. Phares; James P. Wacker
2015-01-01
The National Historic Covered Bridge Preservation Program (NHCBP), sponsored by the Federal Highway Administration (FHWA), was established to preserve the covered timber bridge structures that were constructed in the early 1800s. Today, many of the approximately 880 covered timber bridges still in existence in the United States are closed to vehicular traffic;...
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Mono-manganese mechanism of the photosystem II water splitting reaction by a unique Mn4Ca cluster.
Kusunoki, Masami
2007-06-01
The molecular mechanism of the water oxidation reaction in photosystem II (PSII) of green plants remains a great mystery in life science. This reaction is known to take place in the oxygen evolving complex (OEC) incorporating four manganese, one calcium and one chloride cofactors, that is light-driven to cycle four intermediates, designated S(0) through S(4), to produce four protons, five electrons and lastly one molecular oxygen, for indispensable resources in biosphere. Recent advancements of X-ray crystallography models established the existence of a catalytic Mn(4)Ca cluster ligated by seven protein amino acids, but its functional structure is not yet resolved. The (18)O exchange rates of two substrate water molecules were recently measured for four S(i)-state samples (i=0-3) leading to (34)O(2) and (36)O(2) formations, revealing asymmetric substrate binding sites significantly depending on the S(i)-state. In this paper, we present a chemically complete model for the Mn(4)Ca cluster and its surrounding enzyme field, which we found out from some possible models by using the hybrid density functional theoretic geometry optimization method to confirm good agreements with the 3.0 A resolution PSII model [B. Loll, J. Kern, W. Saenger, A. Zouni , J. Biesiadka, Nature 438 (2005) 1040-1044] and the S-state dependence of (18)O exchange rates [W. Hillier and T. Wydrzynski, Phys. Chem. Chem. Phys. 6 (2004) 4882-4889]. Furthermore, we have verified that two substrate water molecules are bound to asymmetric cis-positions on the terminal Mn ion being triply bridged (mu-oxo, mu-carboxylato, and a hydrogen bond) to the Mn(3)CaO(3)(OH) core, by developing a generalized theory of (18)O exchange kinetics in OEC to obtain an experimental evidence for the cross exchange pathway from the slow to the fast exchange process. Some important experimental data will be discussed in terms of this model and its possible tautomers, to suggest that a cofactor, Cl(-) ion, may be bound to CP43-Arg357 nearby Ca(2+) ion and that D1-His337 may be used to trap a released proton only in the S(2)-state.
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Toll bridge report to the California legislature, FY 2001 - 02
DOT National Transportation Integrated Search
2002-01-01
In the San Francisco Bay Area, eight toll bridges serve as essential transportation arteries for the traveling public. Seven of the bridges are state-owned: the Antioch Bridge, the Benicia-Martinez Bridge, the Carquinez Bridge, the Richmond-San Rafae...
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Economic impact of closing low-volume rural bridges.
DOT National Transportation Integrated Search
2013-02-01
The State of Kansas has approximately 25,464 bridges that are located on the state, county and : city roadway network. As the infrastructure in Kansas ages, bridges can become structurally : deficient or functionally obsolete. The Kansas Department o...
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Topographic view of the Spring Creek Bridge and Collier State ...
Topographic view of the Spring Creek Bridge and Collier State Park, view looking east. - Spring Creek Bridge, Spanning Spring Creek at Milepoint 253.98 on Oregon to California Highway (US Route 97), Chiloquin, Klamath County, OR
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322. Dennis Hill, Photographer May 1998 VIEW OF GIRDER SPANS, ...
322. Dennis Hill, Photographer May 1998 VIEW OF GIRDER SPANS, OAKLAND APPROACH AT TRANSITION TO DOUBLE-DECK ROADWAY, FACING WEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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View of deck of pony truss approach span. Deck system ...
View of deck of pony truss approach span. Deck system has failed at northwest corner. Looking south. - Naval Supply Annex Stockton, Daggett Road Bridge, Daggett Road traversing Burns Cut Off, Stockton, San Joaquin County, CA
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77 FR 14420 - Landmarks Committee of the National Park System Advisory Board Meeting
Federal Register 2010, 2011, 2012, 2013, 2014
2012-03-09
...: Nominations California KNIGHT'S FERRY BRIDGE, Stanislaus County, CA U.S. COURT HOUSE AND POST OFFICE (COURT... SANGRE DE CRISTO LAND GRANT HISTORIC DISTRICT, Costilla County, CO Kansas BLACK JACK BATTLEFIELD, Douglas...
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22. AERIAL VIEW OF ARROYO SECO PARKWAY AT ELYSIAN PARK ...
22. AERIAL VIEW OF ARROYO SECO PARKWAY AT ELYSIAN PARK SHOWING FIGUEROA STREET TUNNELS. NOTE PARK ROW BRIDGE AT CENTER. LOOKING NE. - Arroyo Seco Parkway, Los Angeles to Pasadena, Los Angeles, Los Angeles County, CA
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24. AERIAL VIEW OF ARROYO SECO PARKWAY AT ELYSIAN PARK ...
24. AERIAL VIEW OF ARROYO SECO PARKWAY AT ELYSIAN PARK SHOWING FIGUEROA STREET TUNNELS. NOTE PARK ROW BRIDGE AT BOTTOM. LOOKING NE. - Arroyo Seco Parkway, Los Angeles to Pasadena, Los Angeles, Los Angeles County, CA
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21. AERIAL VIEW OF ARROYO SECO PARKWAY AT ELYSIAN PARK ...
21. AERIAL VIEW OF ARROYO SECO PARKWAY AT ELYSIAN PARK SHOWING FIGUEROA STREET TUNNELS. NOTE PARK ROW BRIDGE AT TOP. LOOKING NE. - Arroyo Seco Parkway, Los Angeles to Pasadena, Los Angeles, Los Angeles County, CA
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23. AERIAL VIEW OF ARROYO SECO PARKWAY AT ELYSIAN PARK ...
23. AERIAL VIEW OF ARROYO SECO PARKWAY AT ELYSIAN PARK SHOWING FIGUEROA STREET TUNNELS. NOTE PARK ROW BRIDGE AT TOP. LOOKING NE. - Arroyo Seco Parkway, Los Angeles to Pasadena, Los Angeles, Los Angeles County, CA
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2. Photocopy of a drawing (original in the Collection of ...
2. Photocopy of a drawing (original in the Collection of the PL&C, Shelf 118, Drawing 272)--ca. 1825--PROPOSAL OF BUILDING EASTERN CANAL - Eastern Canal, Bridge & Armory Streets vicinity, Lowell, Middlesex County, MA
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287. Dennis Hill, Photographer July 1998 DETAIL VIEW OF UPPER ...
287. Dennis Hill, Photographer July 1998 DETAIL VIEW OF UPPER CHORD OF THROUGH TRUSS AT PANEL POINT, FACING NORTHWEST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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78 FR 15879 - Drawbridge Operation Regulations; Sacramento River, Sacramento, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2013-03-13
... Loser'' 5K walk and run event. This deviation allows the bridge to remain in the closed-to-navigation... the First Annual ``Biggest Loser'' 5K walk and run event. This temporary deviation has been...
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76 FR 11679 - Drawbridge Operation Regulation; Sacramento River, Sacramento, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2011-03-03
... 7th Annual Shamrock Half Marathon. This deviation allows the bridge to remain in the closed-to... March 13, 2011 to allow the community to participate in the 7th Annual Shamrock Half Marathon. This...
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77 FR 10372 - Drawbridge Operation Regulation; Sacramento River, Sacramento, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2012-02-22
... 8th Annual Shamrock Half Marathon. This deviation allows the bridge to remain in the closed-to... March 11, 2012 to allow the community to participate in the 8th Annual Shamrock Half Marathon. This...
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Detail of metal caisson and decking system on pony truss ...
Detail of metal caisson and decking system on pony truss span. From navy land. Looking southeast. - Naval Supply Annex Stockton, Daggett Road Bridge, Daggett Road traversing Burns Cut Off, Stockton, San Joaquin County, CA
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View of one half of movable span, showing truss and ...
View of one half of movable span, showing truss and tension bars, from navy land looking southwest. - Naval Supply Annex Stockton, Daggett Road Bridge, Daggett Road traversing Burns Cut Off, Stockton, San Joaquin County, CA
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Murphy, Julia; Bindman, Andrew B.
2015-01-01
Policymakers have increasingly promoted health services integration to improve quality and efficiency. The US health care safety net, which comprises providers of health care to uninsured, Medicaid, and other vulnerable patients, remains a largely fragmented collection of providers. We interviewed leadership from safety net hospitals and community health centers in 5 US cities (Boston, MA; Denver, CO; Los Angeles, CA; Minneapolis, MN; and San Francisco, CA) throughout 2013 on their experiences with service integration. We identify conflicts in organizational mission, identity, and consumer orientation that have fostered reluctance to enter into collaborative arrangements. We describe how smaller scale initiatives, such as capitated model for targeted populations, health information exchange, and quality improvements led by health plans, can help bridge cultural differences to lay the groundwork for developing integrated care programs. PMID:26509286
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NASA Astrophysics Data System (ADS)
Tong, Kefeng; Song, Xingfu; Sun, Shuying; Xu, Yanxia; Yu, Jianguo
2014-08-01
All-atom molecular dynamics simulations were employed to provide microscopic mechanism for the salt tolerance of polyelectrolytes dispersants. The conformational variation of polyelectrolytes and interactions between COO- groups and counterions/water molecules were also studied via radius of gyration and pair correlations functions. Sodium polyacrylate (NaPA) and sodium salts of poly(acrylic acid)-poly(ethylene oxide) (NaPA-PEO) were selected as the representative linear and comb-like polyelectrolyte, respectively. The results show that Ca2+ ions interact with COO- groups much stronger than Na+ ions and can bring ion-bridging interaction between intermolecular COO- groups in the NaPA systems. While in the NaPA-PEO systems, the introduced PEO side chains can prevent backbone chains from ion-bridging interactions and weaken the conformational changes. The present results can help in selecting and designing new-type efficient polyelectrolyte dispersants with good salt tolerance.
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Pérez Navarro, Montserrat; Ames, William M.; Nilsson, Håkan; Lohmiller, Thomas; Pantazis, Dimitrios A.; Rapatskiy, Leonid; Nowaczyk, Marc M.; Neese, Frank; Boussac, Alain; Messinger, Johannes; Lubitz, Wolfgang; Cox, Nicholas
2013-01-01
The assignment of the two substrate water sites of the tetra-manganese penta-oxygen calcium (Mn4O5Ca) cluster of photosystem II is essential for the elucidation of the mechanism of biological O-O bond formation and the subsequent design of bio-inspired water-splitting catalysts. We recently demonstrated using pulsed EPR spectroscopy that one of the five oxygen bridges (μ-oxo) exchanges unusually rapidly with bulk water and is thus a likely candidate for one of the substrates. Ammonia, a water analog, was previously shown to bind to the Mn4O5Ca cluster, potentially displacing a water/substrate ligand [Britt RD, et al. (1989) J Am Chem Soc 111(10):3522–3532]. Here we show by a combination of EPR and time-resolved membrane inlet mass spectrometry that the binding of ammonia perturbs the exchangeable μ-oxo bridge without drastically altering the binding/exchange kinetics of the two substrates. In combination with broken-symmetry density functional theory, our results show that (i) the exchangable μ-oxo bridge is O5 {using the labeling of the current crystal structure [Umena Y, et al. (2011) Nature 473(7345):55–60]}; (ii) ammonia displaces a water ligand to the outer manganese (MnA4-W1); and (iii) as W1 is trans to O5, ammonia binding elongates the MnA4-O5 bond, leading to the perturbation of the μ-oxo bridge resonance and to a small change in the water exchange rates. These experimental results support O-O bond formation between O5 and possibly an oxyl radical as proposed by Siegbahn and exclude W1 as the second substrate water. PMID:24023065
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Code of Federal Regulations, 2011 CFR
2011-04-01
... 19 Customs Duties 1 2011-04-01 2011-04-01 false Equipment and materials for constructing bridges... WITH CANADA AND MEXICO International Traffic § 123.18 Equipment and materials for constructing bridges.... Equipment for use in construction of bridges or tunnels between the United States and Canada or Mexico shall...
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Durability and Wood Protection for Historic Covered Bridges in the United States
Vina W. Yang; Carol A. Clausen
2014-01-01
A majority of the covered wooden bridges in United States were built in the mid -1800âs. These structures represent a unique cultural and technological heritage from that era. Over time, these bridges have been deteriorated by microorganisms and insects or damaged by acts of vandalism and arson. The National Historic Covered Bridge Preservation (NHCBP) Program...
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7. DETAIL VIEW OF BRIDGE DATEPLATE WHICH READS '1929, WHITE ...
7. DETAIL VIEW OF BRIDGE DATEPLATE WHICH READS '1929, WHITE RIVER BRIDGE, BUILT BY ARKANSAS HIGHWAY COMMISSION, DWIGHT BLACKWOOD, CHAIRMAN, JUSTIN MATTHEWS, J. LAN WILLIAMS, J.S. PARKS, SAM J. WILSON, COMMISSIONERS, C.S. CHRISTIAN, STATE HIGHWAY ENGINEER, IRA HEDRICK, INC., CONSULTING ENGINEERS, LIST & WEATHERLY, CONSTRUCTION CO.' - Newport Bridge, Spanning White River at State Highway 14, Newport, Jackson County, AR
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50 CFR 17.108 - List of designated manatee protection areas.
Code of Federal Regulations, 2013 CFR
2013-10-01
... the Granada Bridge (State Road 40) (including the Tomoka Basin), a distance of approximately 5.0 km (3... feet) north to 305 meters (1,000 feet) south of the Granada Bridge (State Road 40), a distance of... point 305 meters (1,000 feet) south of the Granada Bridge (State Road 40) to a point 152 meters (500...
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50 CFR 17.108 - List of designated manatee protection areas.
Code of Federal Regulations, 2014 CFR
2014-10-01
... the Granada Bridge (State Road 40) (including the Tomoka Basin), a distance of approximately 5.0 km (3... feet) north to 305 meters (1,000 feet) south of the Granada Bridge (State Road 40), a distance of... point 305 meters (1,000 feet) south of the Granada Bridge (State Road 40) to a point 152 meters (500...
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50 CFR 17.108 - List of designated manatee protection areas.
Code of Federal Regulations, 2012 CFR
2012-10-01
... the Granada Bridge (State Road 40) (including the Tomoka Basin), a distance of approximately 5.0 km (3... feet) north to 305 meters (1,000 feet) south of the Granada Bridge (State Road 40), a distance of... point 305 meters (1,000 feet) south of the Granada Bridge (State Road 40) to a point 152 meters (500...
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7. Photo copy of drawing, October 10, 1930. STATE BOARD ...
7. Photo copy of drawing, October 10, 1930. STATE BOARD OF PUBLIC ROADS, BRIDGE DEPARTMENT, GREENWOOD R.R. BRIDGE, WARWICK, R.I., SECTIONS. Drawing no. unknown. Rhode Island State Department of Transportation, Smith Street, Providence, Rhode Island. - Greenwood Railroad Bridge, Post Road (U.S. Route 1) & Main Avenue over New Haven Railroad, Warwick, Kent County, RI
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8. Photo copy of drawing, October 10, 1930. STATE BOARD ...
8. Photo copy of drawing, October 10, 1930. STATE BOARD OF PUBLIC ROADS, BRIDGE DEPARTMENT, GREENWOOD R.R. BRIDGE, WARWICK, R.I., TRUSS. Drawing no. 2.014. Rhode Island State Department of Transportation, Smith Street, Providence, Rhode Island. - Greenwood Railroad Bridge, Post Road (U.S. Route 1) & Main Avenue over New Haven Railroad, Warwick, Kent County, RI
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Parryville Bridge (S.R. 2008, section 01B Bridge) looking north across ...
Parryville Bridge (S.R. 2008, section 01B Bridge) looking north across traffic level, showing village of Parryville. - Parryville Bridge, State Route 2008 over Pohapoco Creek, Parryville, Carbon County, PA
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Corrosion resistance of grouted post-tensioning systems : [summary].
DOT National Transportation Integrated Search
2013-08-01
The increased popularity of post-tensioned bridge construction in the United States has led to concerns about corrosion and its impact on the life cycle of these bridges. Although the vast majority of post-tensioned bridges in the United States have ...
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Impact of bridge ratings on the timber transportation industry.
DOT National Transportation Integrated Search
2009-08-01
The logging industry plays an important role in the State of Wisconsins economy. Additionally, the condition of some of Wisconsins bridges, as : well as the state of our nations bridges, is deteriorated due to lack of funding for replacement...
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DOE Office of Scientific and Technical Information (OSTI.GOV)
Killian, Christopher; Metzler, Rebecca; Gong, Y. U. T.
Sea urchin teeth are remarkable and complex calcite structures, continuously growing at the forming end and self-sharpening at the mature grinding tip. The calcite (CaCO{sub 3}) crystals of tooth components, plates, fibers, and a high-Mg polycrystalline matrix, have highly co-oriented crystallographic axes. This ability to co-orient calcite in a mineralized structure is shared by all echinoderms. However, the physico-chemical mechanism by which calcite crystals become co-oriented in echinoderms remains enigmatic. Here, we show differences in calcite c-axis orientations in the tooth of the purple sea urchin (Strongylocentrotus purpuratus), using high-resolution X-ray photoelectron emission spectromicroscopy (X-PEEM) and microbeam X-ray diffraction ({mu}XRD).more » All plates share one crystal orientation, propagated through pillar bridges, while fibers and polycrystalline matrix share another orientation. Furthermore, in the forming end of the tooth, we observe that CaCO{sub 3} is present as amorphous calcium carbonate (ACC). We demonstrate that co-orientation of the nanoparticles in the polycrystalline matrix occurs via solid-state secondary nucleation, propagating out from the previously formed fibers and plates, into the amorphous precursor nanoparticles. Because amorphous precursors were observed in diverse biominerals, solid-state secondary nucleation is likely to be a general mechanism for the co-orientation of biomineral components in organisms from different phyla.« less
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Mechanism of calcite co-orientation in the sea urchin tooth.
Killian, Christopher E; Metzler, Rebecca A; Gong, Y U T; Olson, Ian C; Aizenberg, Joanna; Politi, Yael; Wilt, Fred H; Scholl, Andreas; Young, Anthony; Doran, Andrew; Kunz, Martin; Tamura, Nobumichi; Coppersmith, Susan N; Gilbert, P U P A
2009-12-30
Sea urchin teeth are remarkable and complex calcite structures, continuously growing at the forming end and self-sharpening at the mature grinding tip. The calcite (CaCO(3)) crystals of tooth components, plates, fibers, and a high-Mg polycrystalline matrix, have highly co-oriented crystallographic axes. This ability to co-orient calcite in a mineralized structure is shared by all echinoderms. However, the physico-chemical mechanism by which calcite crystals become co-oriented in echinoderms remains enigmatic. Here, we show differences in calcite c-axis orientations in the tooth of the purple sea urchin ( Strongylocentrotus purpuratus ), using high-resolution X-ray photoelectron emission spectromicroscopy (X-PEEM) and microbeam X-ray diffraction (muXRD). All plates share one crystal orientation, propagated through pillar bridges, while fibers and polycrystalline matrix share another orientation. Furthermore, in the forming end of the tooth, we observe that CaCO(3) is present as amorphous calcium carbonate (ACC). We demonstrate that co-orientation of the nanoparticles in the polycrystalline matrix occurs via solid-state secondary nucleation, propagating out from the previously formed fibers and plates, into the amorphous precursor nanoparticles. Because amorphous precursors were observed in diverse biominerals, solid-state secondary nucleation is likely to be a general mechanism for the co-orientation of biomineral components in organisms from different phyla.
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Multiple C-terminal tail Ca2+/CaMs regulate CaV1.2 function but do not mediate channel dimerization
Kim, Eun Young; Rumpf, Christine H; Van Petegem, Filip; Arant, Ryan J; Findeisen, Felix; Cooley, Elizabeth S; Isacoff, Ehud Y; Minor, Daniel L
2010-01-01
Interactions between voltage-gated calcium channels (CaVs) and calmodulin (CaM) modulate CaV function. In this study, we report the structure of a Ca2+/CaM CaV1.2 C-terminal tail complex that contains two PreIQ helices bridged by two Ca2+/CaMs and two Ca2+/CaM–IQ domain complexes. Sedimentation equilibrium experiments establish that the complex has a 2:1 Ca2+/CaM:C-terminal tail stoichiometry and does not form higher order assemblies. Moreover, subunit-counting experiments demonstrate that in live cell membranes CaV1.2s are monomers. Thus, contrary to previous proposals, the crystallographic dimer lacks physiological relevance. Isothermal titration calorimetry and biochemical experiments show that the two Ca2+/CaMs in the complex have different properties. Ca2+/CaM bound to the PreIQ C-region is labile, whereas Ca2+/CaM bound to the IQ domain is not. Furthermore, neither of lobes of apo-CaM interacts strongly with the PreIQ domain. Electrophysiological studies indicate that the PreIQ C-region has a role in calcium-dependent facilitation. Together, the data show that two Ca2+/CaMs can bind the CaV1.2 tail simultaneously and indicate a functional role for Ca2+/CaM at the C-region site. PMID:20953164
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Kim, Eun Young; Rumpf, Christine H; Van Petegem, Filip; Arant, Ryan J; Findeisen, Felix; Cooley, Elizabeth S; Isacoff, Ehud Y; Minor, Daniel L
2010-12-01
Interactions between voltage-gated calcium channels (Ca(V)s) and calmodulin (CaM) modulate Ca(V) function. In this study, we report the structure of a Ca(2+)/CaM Ca(V)1.2 C-terminal tail complex that contains two PreIQ helices bridged by two Ca(2+)/CaMs and two Ca(2+)/CaM-IQ domain complexes. Sedimentation equilibrium experiments establish that the complex has a 2:1 Ca(2+)/CaM:C-terminal tail stoichiometry and does not form higher order assemblies. Moreover, subunit-counting experiments demonstrate that in live cell membranes Ca(V)1.2s are monomers. Thus, contrary to previous proposals, the crystallographic dimer lacks physiological relevance. Isothermal titration calorimetry and biochemical experiments show that the two Ca(2+)/CaMs in the complex have different properties. Ca(2+)/CaM bound to the PreIQ C-region is labile, whereas Ca(2+)/CaM bound to the IQ domain is not. Furthermore, neither of lobes of apo-CaM interacts strongly with the PreIQ domain. Electrophysiological studies indicate that the PreIQ C-region has a role in calcium-dependent facilitation. Together, the data show that two Ca(2+)/CaMs can bind the Ca(V)1.2 tail simultaneously and indicate a functional role for Ca(2+)/CaM at the C-region site.
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Free energy landscape of a minimalist salt bridge model.
Li, Xubin; Lv, Chao; Corbett, Karen M; Zheng, Lianqing; Wu, Dongsheng; Yang, Wei
2016-01-01
Salt bridges are essential to protein stability and dynamics. Despite the importance, there has been scarce of detailed discussion on how salt bridge partners interact with each other in distinct solvent exposed environments. In this study, employing a recent generalized orthogonal space tempering (gOST) method, we enabled efficient molecular dynamics simulation of repetitive breaking and reforming of salt bridge structures within a minimalist salt-bridge model, the Asp-Arg dipeptide and thereby were able to map its detailed free energy landscape in aqueous solution. Free energy surface analysis shows that although individually-solvated states are more favorable, salt-bridge states still occupy a noticeable portion of the overall population. Notably, the competing forces, e.g. intercharge attractions that drive the formation of salt bridges and solvation forces that pull the charged groups away from each other, are energetically comparable. As the result, the salt bridge stability is highly tunable by local environments; for instance when local water molecules are perturbed to interact more strongly with each other, the population of the salt-bridge states is likely to increase. Our results reveal the critical role of local solvent structures in modulating salt-bridge partner interactions and imply the importance of water fluctuations on conformational dynamics that involves solvent accessible salt bridge formations. © 2015 The Protein Society.
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4. ENVIRONMENT, FROM EAST, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE ...
4. ENVIRONMENT, FROM EAST, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE NO. 6051 CARRYING STATE ROUTE 673 (FEATHERBOTTOM ROAD) OVER CATOCTIN CREEK - Virginia Department of Transportation Bridge No. 6051, Spanning Catoctin Creek at State Route 673 (Featherbottom Road), Waterford, Loudoun County, VA
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4. ENVIRONMENT, FROM SOUTHEAST, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE ...
4. ENVIRONMENT, FROM SOUTHEAST, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE NO. 6023 CARRYING STATE ROUTE 646 (ADEN ROAD) OVER RAILROAD TRACKS - Virginia Department of Transportation Bridge No. 6023, Spanning Norfolk Southern tracks at State Route 646, Nokesville, Prince William County, VA
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2. ENVIRONMENT, FROM NORTH, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE ...
2. ENVIRONMENT, FROM NORTH, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE NO. 6051 CARRYING STATE ROUTE 673 (FEATHERBOTTOM ROAD) OVER CATOCTIN CREEK - Virginia Department of Transportation Bridge No. 6051, Spanning Catoctin Creek at State Route 673 (Featherbottom Road), Waterford, Loudoun County, VA
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3. ENVIRONMENT, FROM SOUTH, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE ...
3. ENVIRONMENT, FROM SOUTH, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE NO. 6051 CARRYING STATE ROUTE 673 (FEATHERBOTTOM ROAD) OVER CATOCTIN CREEK - Virginia Department of Transportation Bridge No. 6051, Spanning Catoctin Creek at State Route 673 (Featherbottom Road), Waterford, Loudoun County, VA
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1. ENVIRONMENT, FROM NORTHWEST, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE ...
1. ENVIRONMENT, FROM NORTHWEST, SHOWING VIRGINIA DEPARTMENT OF TRANSPORTATION BRIDGE NO. 6051 CARRYING STATE ROUTE 673 (FEATHERBOTTOM ROAD) ACROSS CATOCTIN CREEK - Virginia Department of Transportation Bridge No. 6051, Spanning Catoctin Creek at State Route 673 (Featherbottom Road), Waterford, Loudoun County, VA
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Developing cost effective plans for low volume bridges
DOT National Transportation Integrated Search
2006-09-01
There is currently an escalating concern across the state of Kansas with respect to the age : and condition of low volume bridges and methods available to modify or replace them. A : high percentage of low volume bridges in the state of Kansas requir...
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Synthesis and evaluation of the service limit state of engineered fills for bridge support.
DOT National Transportation Integrated Search
2016-02-02
This report synthesizes the current service limit state (SLS) design and analyses of engineered fills for bridge support used as shallow foundations. The SLS for settlement and deformations of bridge supports are summarized. Extensive literature revi...
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23 CFR 650.307 - Bridge inspection organization.
Code of Federal Regulations, 2011 CFR
2011-04-01
... 23 Highways 1 2011-04-01 2011-04-01 false Bridge inspection organization. 650.307 Section 650.307... BRIDGES, STRUCTURES, AND HYDRAULICS National Bridge Inspection Standards § 650.307 Bridge inspection... bridges located on public roads that are fully or partially located within the State's boundaries, except...
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Condition Assessment of Iowa Timber Bridges Using Advanced Inspection Tools
Travis Hosteng; James Wacker; Brent Phares
2013-01-01
This paper reports the results of a study undertaken to assess the condition of timber bridges in the state of Iowa. This study was carried out as part of an overall national study spearheaded by the Forest Products Laboratory and FHWA to investigate the field performance of timber bridges throughout the United States. In the U.S., Iowa has the most timber bridge...
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6. Photo copy of drawing, October 10, 1930. STATE BOARD ...
6. Photo copy of drawing, October 10, 1930. STATE BOARD OF PUBLIC ROADS, BRIDGE DEPARTMENT, GREENWOOD R.R. BRIDGE, WARWICK, R.I., GENERAL PLAN. Drawing no. 2.006. Rhode Island State Department of Transportation, Smith Street, Providence, Rhode Island. - Greenwood Railroad Bridge, Post Road (U.S. Route 1) & Main Avenue over New Haven Railroad, Warwick, Kent County, RI
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23 CFR 661.49 - Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges?
Code of Federal Regulations, 2012 CFR
2012-04-01
... 23 Highways 1 2012-04-01 2012-04-01 false Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? 661.49 Section 661.49 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT... Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? Yes. Interstate...
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23 CFR 661.49 - Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges?
Code of Federal Regulations, 2014 CFR
2014-04-01
... 23 Highways 1 2014-04-01 2014-04-01 false Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? 661.49 Section 661.49 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT... Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? Yes. Interstate...
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23 CFR 661.49 - Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges?
Code of Federal Regulations, 2013 CFR
2013-04-01
... 23 Highways 1 2013-04-01 2013-04-01 false Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? 661.49 Section 661.49 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT... Can IRRBP funds be spent on Interstate, State Highway, and Toll Road IRR bridges? Yes. Interstate...
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Deck view, west approach; former Western Pacific (now Union Pacific) ...
Deck view, west approach; former Western Pacific (now Union Pacific) Railroad at left; wind turbine generators atop hill in background; view to northeast; 90mm lens - Carroll Overhead Bridge, Altamont Pass Road, Livermore, Alameda County, CA
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INTERIOR OF WEST SPAN LOOKING WEST (SHADOW OF VERTICAL LAPS ...
INTERIOR OF WEST SPAN LOOKING WEST (SHADOW OF VERTICAL LAPS PLACED ON ZONE III; ASPHALT ZONE IX) - Honey Run Bridge, Spanning Butte Creek, bypassed section of Honey Run Road (originally Carr Hill Road), Paradise, Butte County, CA
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9. Detail, original door in south leanto. The current project ...
9. Detail, original door in south lean-to. The current project will modify this opening to allow handicap access. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA
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23. 100 foot through truss looking west from the ...
23. 100 foot through truss - looking west from the downstream side, view of a single through truss showing its general arrangement on extended column piers. - Weidemeyer Bridge, Spanning Thomes Creek at Rawson Road, Corning, Tehama County, CA
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133. Dennis Hill, Photographer January 1998 VIEW OF TRANSBAY TERMINAL ...
133. Dennis Hill, Photographer January 1998 VIEW OF TRANSBAY TERMINAL BUS LOOP FROM HOWARD STREET BETWEEN FIRST AND SECOND STREETS, FACING EAST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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215. Dennis Hill, Photographer May 1998 DETAIL VIEW OF STRAND ...
215. Dennis Hill, Photographer May 1998 DETAIL VIEW OF STRAND SHOES AND STORM CABLE EYE BARS IN YERBA BUENA ANCHORAGE, FACING EAST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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345. Caltrans, Photographer September 20, 1935 "WEST ANCHOR ARM"; DETAIL ...
345. Caltrans, Photographer September 20, 1935 "WEST ANCHOR ARM"; DETAIL VIEW OF CANTILEVER TRUSS WEST ANCHOR ARM UNDER CONSTRUCTION. 7-1023 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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262. Frank Deras Jr., Photographer June 1998 AERIAL VIEW OF ...
262. Frank Deras Jr., Photographer June 1998 AERIAL VIEW OF CANTILEVER TRUSS CANTILEVER ARM AND SUSPENDED SPAN, NORTH SIDE, FACING SOUTH. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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258. Dennis Hill, Photographer April 1998 VIEW OF CANTILEVER TRUSS ...
258. Dennis Hill, Photographer April 1998 VIEW OF CANTILEVER TRUSS ANCHOR ARM AT PIERS E- AND E-2, SOUTH SIDE, FACING NORTH. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA
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Load ratings for secondary bridges.
DOT National Transportation Integrated Search
1991-05-01
A load rating states the load in tons which a vehicle can impose on a bridge. : Changes in guidelines, standards, and customary uses of bridges require : analyses of bridges to be updated and re-evaluated. : In this report, twenty-two secondary bridg...
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Effect of Hindlimb Unloading on Rat Soleus Fiber Force, Stiffness, and Calcium Sensitivity
NASA Technical Reports Server (NTRS)
McDonald Kerry S.; Fitts, Robert H.
1995-01-01
The purpose of this study was to examine the time course of change in soleus muscle fiber peak force (N), tension (P(sub 0), kN/sq m), elastic modulus (E(sub 0)), and force-pCa and stiffness - pCa relationships. After 1, 2, or 3 wk of Hindlimb Unloading (HU), single fibers were isolated and placed between a motor arm and a transducer, and fiber diameter, peak absolute force, P(sub 0), E(sub 0), and force-pCa and stiffness-pca relationships were characterized. One week of HU resulted in a significant reduction in fiber diameter (68 +/- 2 vs. 57 +/- 1 micrometer), force (3.59 +/- 0.15 vs. 2.19 +/- 0.12 x 10(exp -4) N), P(sub 0) (102 +/- 4 vs. 85 +/- 2 kN/sq m), and E(sub 0) (1.96 +/- 0.12 vs. 1.37 +/- 0.13 X 10(exp 7) N/sq m) and 2 wk of HU caused a further decline in fiber diameter (45 +/- 1 micrometer), force (1.31 +/- 0.06 x 10(exp -4) N), and E(sub 0)(0.96 +/- 0.09 x 10(exp 7) N/sq m). Although the mean fiber diameter and absolute force continued to decline through 3 wk of HU, P(sub 0) recovered to values not significantly different from control. The P(sub 0)/E(sub 0) ratio was significantly increased after 1 (5.5 +/- 0.3 to 7.1 +/- 0.6), 2, and 3 wk of HU, and the 2-wk (9.5 +/- 0.4) and 3-wk (9.4 +/- 0.8) values were significantly greater than the 1-wk values. The force-pCa and stiffness-pCa curves were shifted right- ward after 1, 2, and 3 wk of HU. At 1 wk of HU, the Ca(2+) sensitivity of isometric force, assessed by Ca(2+) concentration required for half-maximal force, was increased from the control value of 1.83 +/- 0.12 to 2.30 +/- 0.10 micrometers. In conclusion, after HU, the decrease in soleus fiber P(sub 0) can be explained by a reduction in the number of myofibrillar cross bridges per cross-sectional area. Our working hypothesis is that the loss of contractile protein reduces the number of cross bridges per cross-sectional area and increases the filament lattice spacing. The increased spacing reduces cross-bridge force and stiffness, but P(sub 0)/E(sub 0) increases because of a quantitatively greater effect on stiffness.
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Shi, Da-Chuan; Wang, Juan; Hu, Rui-Bo; Zhou, Gong-Ke; O'Neill, Malcolm A; Kong, Ying-Zhen
2017-06-01
The structure of a pectin network requires both calcium (Ca 2+ ) and boron (B). Ca 2+ is involved in crosslinking pectic polysaccharides and arbitrarily induces the formation of an "egg-box" structure among pectin molecules, while B crosslinks rhamnogalacturonan II (RG-II) side chain A apiosyl residues in primary cell walls to generate a borate-dimeric-rhamnogalacturonan II (dRG-II-B) complex through a boron-bridge bond, leading to the formation of a pectin network. Based on recent studies of dRG-II-B structures, a hypothesis has been proposed suggesting that Ca 2+ is a common component of the dRG-II-B complex. However, no in vivo evidence has addressed whether B affects the stability of Ca 2+ crosslinks. Here, we investigated the L-fucose-deficient dwarf mutant mur1, which was previously shown to require exogenous B treatment for phenotypic reversion. Imbibed Arabidopsis thaliana seeds release hydrated polysaccharides to form a halo of seed mucilage covering the seed surface, which consists of a water-soluble outer layer and an adherent inner layer. Our study of mur1 seed mucilage has revealed that the pectin in the outer layer of mucilage was relocated to the inner layer. Nevertheless, the mur1 inner mucilage was more vulnerable to rough shaking or ethylene diamine tetraacetic acid (EDTA) extraction than that of the wild type. Immunolabeling analysis suggested that dRG-II-B was severely decreased in mur1 inner mucilage. Moreover, non-methylesterified homogalacturonan (HG) exhibited obvious reassembly in the mur1 inner layer compared with the wild type, which may imply a possible connection between dRG-II-B deficiency and pectin network transformation in the seed mucilage. As expected, the concentration of B in the mur1 inner mucilage was reduced, whereas the distribution and concentration of Ca 2+ in the inner mucilage increased significantly, which could be the reason why pectin relocates from the outer mucilage to the inner mucilage. Consequently, the disruption of B bridges appears to result in the extreme sensitivity of the mur1 mucilage pectin complex to EDTA extraction, despite the reinforcement of the pectin network by excessive Ca 2+ . Therefore, we propose a hypothesis that B, in the form of dRG-II-B, works together with Ca 2+ to maintain pectin network crosslinks and ultimately the mucilage ultrastructure in seed mucilage. This work may serve to complement our current understanding of mucilage configuration.
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DOT National Transportation Integrated Search
2011-02-25
"Bridge scour refers to the removal of sediments from the bridge foundation by flood. It is the most detrimental cause for the majority of : bridge failures in the United States. In the National Bridge Registry, there are 484,546 highway bridges over...
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Developing cost effective plans for low volume bridges
DOT National Transportation Integrated Search
2006-09-01
There is currently an escalating concern across the state of Kansas with respect to the age and condition of low volume bridges and methods available to modify or replace them. A high percentage of low volume bridges in the state of Kansas require or...
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Graf, Darin C.; Warpinski, Norman R.
1996-01-01
A system for single-phase, steady-state permeability measurements of porous rock utilizes a fluid bridge arrangement analogous to a Wheatstone bridge. The arms of the bridge contain the sample and calibrated flow resistors.
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Gala-Garcia, Alfonso; Teixeira, Karina Imaculada Rosa; Wykrota, Francisco Henrique Lana; Sinisterra, Rubén Dario; Cortés, Maria Esperanza
2010-01-01
The aim of this study was to observe the histopathological pulp response following direct pulp capping of mechanically exposed teeth in rats with a composite of beta-tricalcium phosphate-hydroxyapatite bioceramic (BC) and poly (glycolic)-poly (lactic acid) (PLGA) material or a calcium hydroxide [Ca(OH)2] material, compared to BC alone and a negative control of water. Pulp of the maxillary molars was exposed, followed by capping with the experimental material. The pulpal tissue response was assessed post-operatively at 1, 7, 14 and 30 d, followed by histological analysis. The Ca(OH)2 group exhibited severe acute inflammatory cell infiltration at day 14. However after 30 d, a new hard tissue with macro porous obliteration of the pulp chamber and a characteristic necrotic area had appeared. BC and Ca(OH)2 capping were associated with moderate inflammation and dentinal bridge similar. Meanwhile, in the BC/PLGA composite group, there was moderate inflammatory infiltrate and formation of a dense and complete dentinal bridge. In conclusion, the BC/PLGA composite material showed a large zone of tertiary dentin, and effectively reorganized the dentin-pulp complex.
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San Francisco and Bay Area, CA, USA
1991-05-06
STS039-89-053 (28 April-6 May 1991) --- A 70mm, infrared frame of the city of San Francisco, taken on a clear day. The gray areas represent urban regions, and the red areas are vegetated. Within the city of San Francisco, parks like Golden Gate park and the Presidio at the base of the Golden Gate Bridge easily stand out from the well-developed parts of the city. Major thoroughfares and bridges (Golden Gate and Bay Bridges) are seen as are other landmarks such as Candlestick Park and Alcatraz. The trace of the San Andreas faults show as a straight valley running northerly along the San Francisco peninsula. Good detail is visible in the turbid waters of San Francisco Bay.
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12. RW Meyer Sugar Mill: 18761889. Threeroll sugar mill: oneton ...
12. RW Meyer Sugar Mill: 1876-1889. Three-roll sugar mill: one-ton daily processing capacity. Manufactured by Edwin Maw, Liverpool, England, ca. 1855-1870. View: Historical view, 1934, T.T. Waterman Collection, Hawaiian Sugar Planters' Association, Oahu, Hawaii. Masonry-lined passageway leading to the mill at the center of its circular masonry enclosure. The passageway permitted cane to be carried to the mill and cane trash (bagasse) to be carried away after milling. Bridges over the passageways, not in place, permitted the mill animals to circle and power the mill from above. View shows area prior to substantial overgrowth existing in 1978 views of the area. - R. W. Meyer Sugar Mill, State Route 47, Kualapuu, Maui County, HI
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[Dentinopulpar organ: biological basis of clinical response to Ca(OH)2 application].
Gani, O; Crosa, M E
1989-01-01
We have studied the changes presented by mediate and immediate roentgenographic images of indirect pulp capping and pulpotomies. In the cases of indirect pulp capping it was observed an increase of radiolucidity in the places occupied by Ca(OH)2, and sclerotic dentin was present. In pulpotomies, it was found the dentin bridge, which thickness increases with time. The radiolucidity of pulp chamber occupied by Ca(OH)2 was greater in the long time treatment. The radiopacity of non-vital dentin of walls and floor chamber was increased too. It has suggested that Ca++ ion would have migrated from its place and probably would take part in the synthesis of sclerotic dentin, independently of the vitality of the tissue.
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DOT National Transportation Integrated Search
1994-11-01
NCDOTs Bridge Policy establishes controlling design elements for new and reconstructed bridges on the state road system. It includes information to address sidewalks and bicycle facilities on bridges, including minimum handrail heights and sidewal...
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Kenney, Terry A.; McKinney, Tim S.
2006-01-01
Unique bridge scour mitigation designs using concrete A-Jacks were developed by the Utah Department of Transportation and installed at the Colorado River Bridge at State Road 191 and the Green River Bridge at State Road 19. The U.S. Geological Survey monitored stream reaches at these sites by collecting streambed-topography and water-velocity data from 2003 through 2005. These data were acquired annually from a moving boat with an acoustic Doppler current profiler and a differential global positioning system. Raw unordered data were processed and readied for interpolation into organized datasets with DopplerMacros, a set of computer programs. Processed streambed topography data were geostatistically interpolated by using Ordinary Kriging, and inverse distance weighting interpolation was used in the development of the two-dimensional velocity datasets. These organized datasets of topography and velocity were developed for each survey of the two bridge sites. A comparison of the riverbed topography data for each survey was done. An increase in bed elevation related to the installation of the A-Jacks scour countermeasures is evident at the Colorado River Bridge at State Road 191. The three topographic datasets acquired after the installation at the Green River Bridge at State Road 19 show few changes.
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Small-scale chromospheric jets above a sunspot light bridge
NASA Astrophysics Data System (ADS)
Louis, Rohan E.; Beck, Christian; Ichimoto, Kiyoshi
2014-07-01
Context. The chromosphere above sunspot umbrae and penumbrae shows several different types of fast dynamic events such as running penumbral waves, umbral flashes, and penumbral microjets. Aims: The aim of this paper is to identify the physical driver responsible for the dynamic and small-scale chromospheric jets above a sunspot light bridge. Methods: High-resolution broadband filtergrams of active region NOAA 11271 in Ca ii H and G band were obtained with the Solar Optical Telescope on board Hinode. We identified the jets in the Ca ii H images using a semi-automatic routine and determined their length and orientation. We applied local correlation tracking (LCT) to the G-band images to obtain the photospheric horizontal velocity field. The magnetic field topology was derived from a Milne-Eddington inversion of a simultaneous scan with the Spectropolarimeter. Results: The chromospheric jets consist of a bright, triangular-shaped blob that lies on the light bridge, while the apex of this blob extends into a spike-like structure that is bright against the dark umbral background. Most of the jets have apparent lengths of less than 1000 km and about 30% of the jets have lengths between 1000-1600 km. The jets are oriented within ±35° to the normal of the spine of the light bridge. Most of them are clustered near the central part of the light bridge within a 2'' area. The jets are seen to move rapidly along the light bridge and many of them cannot be identified in successive images taken with a 2 min cadence. The jets are primarily located on one side of the light bridge and are directed into the umbral core. The Stokes profiles at or close to the location of the blobs on the LB exhibit both a significant net circular polarization and multiple components, including opposite-polarity lobes. The magnetic field diverges from the light bridge towards the umbral cores that it separates. The LCT reveals that in the photosphere there is a predominantly uni-directional flow with speeds of 100-150 m s-1 along the light bridge. This unidirectional flow is interrupted by a patch of weak or very small motions on the light bridge which also moves along the light bridge. Conclusions: The dynamic short-lived chromospheric jets above the LB seem to be guided by the magnetic field lines. Reconnection events are a likely trigger for such phenomenon since they occur at locations where the magnetic field changes orientation sharply and where we also observe isolated patches of opposite-polarity magnetic components. We find no clear relation between the jets and the photospheric flow pattern.
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Quantification of cracks in concrete bridge decks in Ohio District 3.
DOT National Transportation Integrated Search
2012-02-01
The development of cracks in reinforced bridge decks is a critical problem, not only in Ohio state, but the whole of United States. Many bridge decks constructed within the last 10 years in Ohio have already shown varying levels and patterns of crack...
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Quantification of cracks in concrete bridge decks in Ohio district 3.
DOT National Transportation Integrated Search
2012-02-01
The development of cracks in reinforced bridge decks is a critical problem, not only in Ohio state, but the whole of United States. Many bridge decks constructed within the last 10 years in Ohio have already shown varying levels and patterns of crack...
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Comparative analysis of design codes for timber bridges in Canada, the United States, and Europe
James Wacker; James (Scott) Groenier
2010-01-01
The United States recently completed its transition from the allowable stress design code to the load and resistance factor design (LRFD) reliability-based code for the design of most highway bridges. For an international perspective on the LRFD-based bridge codes, a comparative analysis is presented: a study addressed national codes of the United States, Canada, and...
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17. General view, 'West End S.P. Inspection Building', view ...
17. General view, 'West End - S.P. Inspection Building', view to east, August 16, 1939. Bridge Yard Interlocking Tower and main line tracks at left, Oil House and storage yard at right. An Interurban Electric Railway car, with power supply pantograph raised, is visible at left of the building, another is just visible inside the building's left (north) bay, and still others are visible in the storage yard at right. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA
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19. 'Transverse Bracing, 3 180'61/2' c. to c. End ...
19. 'Transverse Bracing, 3 - 180'-6-1/2' c. to c. End Pins Sing. Tr. Thro' Spans, C.O. 839 10th Crossing Sacramento River, C.O. 840 11th Crossing Sacramento River, C.O. 841 13th Crossing Sacramento River, Sacramento Division, So. Pac. Co., The Phoenix Bridge Co., C.O's. 839, 840 & 841, Drawing #4, Eng'r - C. Scheidl, Draftsman - B. Heald, Scale 1' = 1'-0', April 13th 1901.' - Southern Pacific Railroad Shasta Route, Bridge No. 310.58, Milepost 310.58, Sims, Shasta County, CA
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Update of bridge design standards in Alabama for AASHTO LRFD seismic design requirements.
DOT National Transportation Integrated Search
2013-11-01
The Alabama Department of Transportation (ALDOT) has been required to update their bridge design to the LRFD Bridge Design Specifications. This transition has resulted in changes to the seismic design standards of bridges in the state. Multiple bridg...
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Energetics of proton release on the first oxidation step in the water-oxidizing enzyme.
Saito, Keisuke; Rutherford, A William; Ishikita, Hiroshi
2015-10-07
In photosystem II (PSII), the Mn4CaO5 cluster catalyses the water splitting reaction. The crystal structure of PSII shows the presence of a hydrogen-bonded water molecule directly linked to O4. Here we show the detailed properties of the H-bonds associated with the Mn4CaO5 cluster using a quantum mechanical/molecular mechanical approach. When O4 is taken as a μ-hydroxo bridge acting as a hydrogen-bond donor to water539 (W539), the S0 redox state best describes the unusually short O4-OW539 distance (2.5 Å) seen in the crystal structure. We find that in S1, O4 easily releases the proton into a chain of eight strongly hydrogen-bonded water molecules. The corresponding hydrogen-bond network is absent for O5 in S1. The present study suggests that the O4-water chain could facilitate the initial deprotonation event in PSII. This unexpected insight is likely to be of real relevance to mechanistic models for water oxidation.
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14. VIEW OF ARROYO SECO CHANNEL AND INTERSTATE I5 OVERCROSSING ...
14. VIEW OF ARROYO SECO CHANNEL AND INTERSTATE I-5 OVERCROSSING SEEN FROM SAN FERNANDO ROAD BRIDGE. LOOKING 10°N. - Arroyo Seco Parkway, Figueroa Street Viaduct, Spanning Los Angeles River, Los Angeles, Los Angeles County, CA
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12. VIEW OF ARROYO SECO CHANNEL SEEN FROM SAN FERNANDO ...
12. VIEW OF ARROYO SECO CHANNEL SEEN FROM SAN FERNANDO BRIDGE. NOTE VIADUCTS AND TUNNEL PORTAL AT REAR. LOOKING 234°SW. - Arroyo Seco Parkway, Figueroa Street Viaduct, Spanning Los Angeles River, Los Angeles, Los Angeles County, CA
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7. Oblique view, east and north side, view to southwest. ...
7. Oblique view, east and north side, view to southwest. Note expansive use of glass to provide natural light to interior. - Interurban Electric Railway Bridge Yard Shop, Interstate 80 at Alameda County Postmile 2.0, Oakland, Alameda County, CA
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78 FR 31414 - Drawbridge Operation Regulation; China Basin, San Francisco, CA
Federal Register 2010, 2011, 2012, 2013, 2014
2013-05-24
... scheduled San Francisco Marathon, a community event. This deviation allows the bridge to remain in the... 6 a.m. to 1:30 p.m. on June 16, 2013, to allow participants in the San Francisco Marathon to cross...
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DETAIL VIEW OF BASE OF CAST IRON TOWER SHOWING THE ...
DETAIL VIEW OF BASE OF CAST IRON TOWER SHOWING THE FABRICATING MARK OF STARBUCK IRON WORKS, TROY, NY - Bidwell Bar Suspension Bridge & Stone Toll House, Near Lake Oroville (moved from fork of Feather River), Oroville, Butte County, CA
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PRIVY, LOOKING NORTHEAST, WITH CAR SHOP NO. 3 IN RIGHT ...
PRIVY, LOOKING NORTHEAST, WITH CAR SHOP NO. 3 IN RIGHT BACKGROUND. BRIDGE FROM PRIVYS THIRD FLOOR TO PLANING MILLS SECOND FLOOR IS VISIBLE AT LEFT. - Southern Pacific, Sacramento Shops, Privy, 111 I Street, Sacramento, Sacramento County, CA
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33 CFR 117.647 - Saginaw River.
Code of Federal Regulations, 2013 CFR
2013-07-01
... Lake State Railway Bridge, mile 3.10, and the Canadian National Railway Bridge, mile 4.94, both in Bay... if at least 12 hours advance notice is provided. (b) The draws of the Independence Bridge, mile 3.88, Liberty Street Bridge, mile 4.99, Veterans Memorial Bridge, mile 5.60, and Lafayette Street Bridge, mile 6...
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33 CFR 117.647 - Saginaw River.
Code of Federal Regulations, 2014 CFR
2014-07-01
... Lake State Railway Bridge, mile 3.10, and the Canadian National Railway Bridge, mile 4.94, both in Bay... if at least 12 hours advance notice is provided. (b) The draws of the Independence Bridge, mile 3.88, Liberty Street Bridge, mile 4.99, Veterans Memorial Bridge, mile 5.60, and Lafayette Street Bridge, mile 6...
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33 CFR 117.647 - Saginaw River.
Code of Federal Regulations, 2012 CFR
2012-07-01
... Lake State Railway Bridge, mile 3.10, and the Canadian National Railway Bridge, mile 4.94, both in Bay... if at least 12 hours advance notice is provided. (b) The draws of the Independence Bridge, mile 3.88, Liberty Street Bridge, mile 4.99, Veterans Memorial Bridge, mile 5.60, and Lafayette Street Bridge, mile 6...
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DOT National Transportation Integrated Search
2013-11-01
The bridge in this study was evaluated and a monitoring system was installed to investigate the effects of heavy loads and : the cost of fatigue for bridges on state highways in Louisiana. Also, this study is used to respond to Louisiana Senate : Con...
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Load distribution and fatigue cost estimates of heavy truck loads on Louisiana state bridges.
DOT National Transportation Integrated Search
2013-11-01
The bridge in this study was evaluated and a monitoring system was installed to investigate the effects of heavy loads and : the cost of fatigue for bridges on state highways in Louisiana. Also, this study is used to respond to Louisiana Senate : Con...
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View of east elevation of Parryville Bridge (S.R. 2008, section ...
View of east elevation of Parryville Bridge (S.R. 2008, section 01B bridge) looking southwest, showing substructure - Parryville Bridge, State Route 2008 over Pohapoco Creek, Parryville, Carbon County, PA
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View of west elevation of Parryville Bridge (S.R. 2008, section ...
View of west elevation of Parryville Bridge (S.R. 2008, section 01B Bridge) looking southeast, showing substructure. - Parryville Bridge, State Route 2008 over Pohapoco Creek, Parryville, Carbon County, PA
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Graf, D.C.; Warpinski, N.R.
1996-08-13
A system is described for single-phase, steady-state permeability measurements of porous rock which utilizes a fluid bridge arrangement analogous to a Wheatstone bridge. The arms of the bridge contain the sample and calibrated flow resistors. 8 figs.