The Chief Joseph Hatchery Program 2013 Annual Report

USGS Publications Warehouse

Baldwin, Casey; Pearl, Andrea; Laramie, Matthew; Rohrback, John; Phillips, Pat; Wolf, Keith

2016-01-01

The Chief Joseph Hatchery is the fourth hatchery obligated under the Grand Coulee Dam/Dry Falls project, originating in the 1940s. Leavenworth, Entiat, and Winthrop National Fish Hatcheries were built and operated as mitigation for salmon blockage at Grand Coulee Dam, but the fourth hatchery was not built, and the obligation was nearly forgotten. After the Colville Tribes successfully collaborated with the United States to resurrect the project, planning of the hatchery began in 2001 and construction was completed in 2013. The monitoring program began in 2012 and adult Chinook Salmon were brought on station for the first time in June 2013. BPA is the primary funding source for CJH, and the Mid-Columbia PUDs (Douglas, Grant and Chelan County) have entered into cost-share agreements with the tribes and BPA in order to meet some of their mitigation obligations. The CJH production level was set at 60% in 2013 in order to train staff and test hatchery facility systems during the first year of operation. Leavenworth National Fish Hatchery (LNFH) provided 422 Spring Chinook broodstock in June, 2013; representing the official beginning of CJH operations. In July and August the CCT used a purse seine vessel to collect 814 summer/fall Chinook as broodstock that were a continuation and expansion of the previous Similkameen Pond program. In-hatchery survival for most life stages exceeded survival targets and, as of April 2014, the program was on track to exceed the 60% production target for its start-up year. The CJH monitoring project collected field data to determine Chinook population status, trend, and hatchery effectiveness centered on five major activities; 1) rotary screw traps (juvenile outmigration, natural-origin smolt PIT tagging) 2) beach seine (naturalorigin smolt PIT tagging) 3) lower Okanogan adult fish pilot weir (adult escapement, proportion of hatchery-origin spawners [pHOS], broodstock) 4) spawning ground surveys (redd and carcass surveys)(viable salmonid population [VSP] parameters) 5) eDNA collection (VSP parameter—distribution/spatial structure). Adult summer/fall Chinook spawning escapement in 2013 was estimated to be 8,193, with more than 6,227 natural-origin spawners, which exceeded the recent five year and long term averages. The values for pHOS (0.24) and proportion of natural influence (PNI) (0.79) in 2013 exceeded the objectives (0.67), but the five year averages fell short of the goals (0.39 and 0.62, respectively). An Annual Program Review (APR) was held in March, 2014 to share hatchery production and monitoring data, review the salmon forecast for the upcoming year, and develop action plans for the hatchery, selective harvest, and monitoring projects. Based on a strong pre-season forecast of 67,500 Upper Columbia summer/fall Chinook, the plan for 2014 is to operate the hatchery at full program levels of 2 million summer/fall Chinook and 900,000 spring Chinook. To maximize PNI, broodstock for the integrated program should Chief Joseph Hatchery Program 2013 Annual Report 3 be 100% natural-origin broodstock (NOB) and CCT should plan to harvest their full allocation with the selective harvest program removing as many adult hatchery Chinook as possible with the purse seine, the weir, and at the hatchery ladder.

Research on Captive Broodstock Programs for Pacific Salmon, 2003-2004 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry A.; Athos, Jaime I.; Dittman, Andrew H.

The success of captive broodstock programs depends on high in-culture survival, appropriate development of the reproductive system, and the behavior and survival of cultured salmon after release, either as adults or juveniles. Continuing captive broodstock research designed to improve technology is being conducted to cover all major life history stages of Pacific salmon. We were able to develop an analytical method for optimizing the detection of spawning events in Chinook salmon using EMG signals. The method developed essentially captured the consistently greater frequency of higher EMG values associated with females cover digging immediately following spawning. However, females implanted with EMGmore » tags retained the majority of their eggs, which significantly reduced their reproductive success compared to non-tagged females. Future work will include increased sample sizes, and modified tagging methods to reduce negative effects on reproductive success. Upper Columbia River sockeye salmon exposed to the odorants PEA, L-threonine, Larginine and L-glutamate were able to learn and remember these odorants as maturing adults up to 2.5 years after exposure. These results suggest that the alevin and smolt stages are both important developmental periods for successful olfactory imprinting. Furthermore, the period of time that fish are exposed to imprinting odors may be important for successful imprinting. Experimental fish exposed to imprinting odors as smolts for six or one weeks successfully imprinted to these odors but imprinting could not be demonstrated in smolts exposed to odors for only one day. A 2-3 C reduction in seawater rearing temperature during the fall and winter prior to final maturation had little effect on reproductive development of spring Chinook salmon. Body size at spawning and total ovary mass were similar between temperature treatments. The percentage of fertilized eggs was significantly higher for females exposed to the ambient temperature compared to those exposed to the chilled temperature. However, the percentage of embryos surviving to the eye-stage, total fecundity, and mean egg mass did not differ between treatments. This work is being continued with larger samples sizes and increased duration of temperature exposure. Exercise during the months prior to final maturation had no detectable effects on fertilization success or embryo viability in Redfish Lake Sockeye. Problems with highly variable or low eyed-embryo survival are most likely due to problems with fertilization. Synchronizing spawn timing between males and females may improve gamete fertility, perhaps by making oocyte maturation and ovulation more readily detectable and synchronous within the individual. Improvements in milt production (using GnRHa) and fertilization protocols have apparently increased fertilization success in Redfish Lake sockeye over previous years. Broodstock treatment with azithromycin immediately prior to spawning can protect against acute challenge with R. salmoninarum. Among fish challenged with 10,000 virulent R. salmoninarum cells per fish, progeny of broodstock treated with azithromycin exhibited significantly greater survival than progeny of sham-treated broodstock. Work on the efficacy of antibiotic treatment and vaccination against BKD before and after smoltification in offspring chinook salmon captive broodstocks is ongoing. To date, the long-term study of inbreeding indicates that the potential for anadromous Chinook salmon to respond rapidly to close inbreeding, with adverse consequences for marine survival and, possibly, growth. The effects of inbreeding expressed during early life history do not reveal significant effects. Overall, the results would support recommendations for initiating artificially propagated populations with sufficient, outbred broodstock and implementing carefully monitored breeding practices to minimize rates of inbreeding during a program's duration.« less

Supplement Analysis for the Grande Ronde Basin Endemic Spring Chinook Salmon Supplementation Program EA (DOE/EA-1173/SA-01)

DOE Office of Scientific and Technical Information (OSTI.GOV)

N /A

2003-12-18

The CTUIR and ODFW propose to expand their monitoring and evaluation for the Grande Ronde spring chinook supplementation program to take additional data on summer steelhead that are trapped at the existing adult collection weirs on the upper Grande Ronde River and Catherine Creek. The weirs are a movable design and are operated seasonally during the adult chinook migration. Bull trout and summer steelhead have been trapped at the weirs since 1997 incidental to the spring chinook broodstock collection activities. Minimal data is recorded on both species as a requirement of the ESA permits, and reported to USFWS and NOAAmore » Fisheries. This supplement analysis covers a minor expansion of the program to collect more extensive life history data on summer steelhead. The weir and trap will be installed 2-3 weeks earlier (early to mid-March) than was previously needed for the spring chinook broodstock collection in order to monitor the summer steelhead migration period. The adult steelhead will be captured in the traps, anesthetized, and measured. Data will be recorded on the date of capture, fork length, sex, markings, and maturity of the fish, and scale and punch tissue samples will be taken for genetic analyses.« less

Grande Ronde Endemic Spring Chinook Salmon Supplementation Program; Satellite Facilities Operation and Maintenance, 2005 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

McLean, Michael L.; Seeger, Ryan; Hewitt, Laurie

2006-01-01

There were 2 acclimation periods at the Catherine Creek Acclimation Facility (CCAF) in 2005. During the early acclimation period, 130,748 smolts were delivered from Lookingglass Hatchery (LGH) on 7 March. This group contained progeny of both the captive (53%) and conventional broodstock programs. The size of the fish at delivery was 23.9 fish/lb. Volitional releases began 14 March 2005 and ended 27 March with an estimated total (based on PIT tag detections of 3,187) of 29,402 fish leaving the raceways. This was 22.5% of the total fish delivered. Fish remaining in the raceways after volitional release were forced out. Hourlymore » detections of PIT-tagged fish showed that most of the fish left around 1900 hours. The size of the fish just before the volitional release was 23.9 and the size of the fish remaining just before the forced release was 23.2 fish/lb. The total mortality for the acclimation period was 204 (0.16%). The total number of fish released from the acclimation facility during the early period was 130,544. During the second acclimation period 59,100 smolts were delivered from LGH on 28 March. This group was comprised entirely of progeny from the conventional broodstock program. The size of the fish at delivery was 21.8 fish/lb. Volitional releases began 3 April 2005 and ended with a force out emergency release on 7 April. The size of the fish just before the volitional release was 21.8. The total mortality for the acclimation period was 64 (0.11 %). The total number of fish released from the acclimation facility during the late period was 59,036. There was only 1 planned acclimation period at the Upper Grande Ronde Acclimation Facility (UGRAF) in 2005. During the early acclimation period 105,418 smolts were delivered from LGH on 8 March. This group was comprised entirely of progeny from the conventional broodstock program. The size of the fish at delivery was 21.0 fish/lb. There was no volitional release in 2005 due to freezing air and water conditions prompting an early release. The total mortality for the acclimation period was 49 (0.05 %). The total number of fish released from the acclimation facility during the late period was 105,369. Maintenance and repair activities were conducted at the acclimation facilities in 2005. Facility maintenance work consisted of snow removal, installation of drainage lines, removal of gravel from intake area, installation of new gate at the CCAF, and complete overhaul of 2 travel trailers. The Catherine Creek Adult Capture Facility (CCACF) was put into operation on 11 February 2005. The first adult summer steelhead was captured on 4 March. A total of 190 adult summer steelhead were trapped and released from 4 March to 16 May 2005. Peak arrival at the trap was the week of 8 April. The first adult spring Chinook salmon was captured at CCACF on 6 May 2005. A total of 226 spring Chinook salmon were trapped from 6 May to 8 July 2005. There were 56 adults and 4 jacks unmarked and 136 adult and 30 jack marked spring Chinook salmon trapped. Peak arrival at the trap was the week of 10 June for the unmarked and marked fish. None of the captive broodstock returns were collected for broodstock. Broodstock was collected systematically over the entire return from 31 May to 6 July 2005. Ten of the 34 broodstock collected and transported from CCACF to LGH were unmarked fish trapped. About 18% of the naturally produced adult males and females trapped were taken to LGH for broodstock. One jack was collected for every 5 adult males that were taken to LGH. A total of 30 age 4 and 5 and 4 age 3 fish were transported to LGH for broodstock. The hatchery component of the broodstock was 66.7%. Five weekly spawning surveys were conducted below the weir on Catherine Creek beginning 30 June 2005. During these surveys no live or dead fish were observed. The trap was removed from Catherine Creek on 3 August 2005. Temperatures at the CCACF ranged from -0.1 C on 14 February to 23.7 C on 21 July. The hourly temperatures at the adult trap during the period of operation showed that the lowest water temperatures usually occurred around 0700 hours and the highest water temperatures usually occurred around 1600 hours. Facility maintenance work at CCACF consisted of construction of a debris barrier in front of the intake, maintenance of weir, and weed abatement. The Upper Grande Ronde Adult Collection Facility (UGRACF) was put into operation on 10 March 2005. The first adult summer steelhead was captured on 8 April. A total of 41 adult summer steelhead were trapped and released from 8 April to 11 May 2005. Peak arrival at the trap was the week of 22 April. The first adult spring Chinook salmon was captured at UGRACF on 31 May 2005. A total of 277 spring Chinook salmon were trapped from 31 May to 3 August 2005. There were 14 adults and no jacks unmarked and 257 adult and 6 jack marked spring Chinook salmon trapped. Peak arrival at the trap for both unmarked and marked fish was 10 June.« less

A review of maturation diets for mud crab genus Scylla broodstock: Present research, problems and future perspective.

PubMed

Azra, Mohamad N; Ikhwanuddin, Mhd

2016-03-01

Study of broodstock maturation diets is important in order to increase the quality of berried females, which indirectly improve the larval quantity in the hatchery production of cultured species. This paper reviewed the studies on the maturation diets for mud crab broodstock, genus Scylla and compared independently to identify their effect on reproductive performance and larval quality. The broodstock is usually caught from the wild and held in the spawning or maturation tank for further use of hatchery seed production. Mud crab broodstock was fed either natural diet, artificial diet or mixed diet. Trash fishes were commonly used as a natural feed for mud crab broodstock; meanwhile artificial diets are from formulated fish meal and various kinds of feed. The results indicated that mud crab broodstock has a high dietary requirement for lipids, fatty acids and protein which are to be used during the maturation and breeding processes. However, the natural diet produce better larval quality compared to the artificial diet. The mixed diet is the better diet which resulted in better reproductive performances such as growth, survival, fecundity and maturation processes. This review also discusses the problems in the previous studies for the potential future research to develop very high quality and cost-effective formulated diet for the enhancement of broodstock and seed production technology. Information from this review can be useful in developing a better quality of crustacean broodstock's diet for commercial hatchery production.

Effects of immobilization by electricity and MS-222 on brown trout broodstock and their progeny

USGS Publications Warehouse

Redman, S.D.; Meinertz, J.R.; Gaikowski, M.P.

1998-01-01

To determine the effects of electrically and chemically induced immobilization on postspawn broodstock and their progeny, age-2 and age-3 female broodstock and age-2 male broodstock of brown trout Salmo trutta were immobilized with electricity or tricaine methanesulfonate (MS-222), stripped of their eggs or milt, and weighed. Eggs taken from electrically immobilized females were fertilized with milt taken from age-2 males that were immobilized with electricity, and eggs taken from females immobilized with MS-222 were fertilized with milt taken from age-2 males that were immobilized with MS-222. After spawning, the mortality and weight of broodstock were compared twice over a 6-month period. Egg viability and growth of offspring fry from each treatment group were also compared. Electricity induced complete and consistent immobilization in brown trout broodstock. Electrically immobilized fish were more easily handled than fish immobilized with MS-222; however, electrically immobilized fish survival (70%) was significantly less than fish immobilized with MS-222 (83%). Broodstock growth differences were only noted at 6 months postexposure, when the mean weight of electrically immobilized fish was slightly less than the weight of fish immobilized with MS-222. Broodstock immobilization by electricity did not reduce egg viability or fry growth.

Research on Captive Broodstock Programs for Pacific Salmon, 2001-2002 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry A.; Tezak, E.P.; Endicott, Rick

In the 2000 Federal Columbia River Power System (FCRPS) Biological Opinion, NMFS identified six populations of steelhead and several salmon populations that had dropped to critically low levels and continue to decline. Following thorough risk-benefit analyses, captive propagation programs for some or all of the steelhead (Oncorhynchus mykiss) populations may be required to reduce the risk of extinction, and more programs may be required in the future. Thus, captive propagation programs designed to maintain or rebuild steelhead populations require intensive and rigorous scientific evaluation, much like the other objectives of BPA Project 1993-056-00 currently underway for chinook (O. tshawytscha) andmore » sockeye salmon (O. nerka). Pacific salmon reared to the adult stage in captivity exhibit poor reproductive performance when released to spawn naturally. Poor fin quality and swimming performance, incomplete development of secondary sex characteristics, changes in maturation timing, and other factors may contribute to reduced spawning success. Improving natural reproductive performance is critical for the success of captive broodstock programs in which adult-release is a primary reintroduction strategy for maintaining ESA-listed populations.« less

A review of maturation diets for mud crab genus Scylla broodstock: Present research, problems and future perspective

PubMed Central

Azra, Mohamad N.; Ikhwanuddin, Mhd

2015-01-01

Study of broodstock maturation diets is important in order to increase the quality of berried females, which indirectly improve the larval quantity in the hatchery production of cultured species. This paper reviewed the studies on the maturation diets for mud crab broodstock, genus Scylla and compared independently to identify their effect on reproductive performance and larval quality. The broodstock is usually caught from the wild and held in the spawning or maturation tank for further use of hatchery seed production. Mud crab broodstock was fed either natural diet, artificial diet or mixed diet. Trash fishes were commonly used as a natural feed for mud crab broodstock; meanwhile artificial diets are from formulated fish meal and various kinds of feed. The results indicated that mud crab broodstock has a high dietary requirement for lipids, fatty acids and protein which are to be used during the maturation and breeding processes. However, the natural diet produce better larval quality compared to the artificial diet. The mixed diet is the better diet which resulted in better reproductive performances such as growth, survival, fecundity and maturation processes. This review also discusses the problems in the previous studies for the potential future research to develop very high quality and cost-effective formulated diet for the enhancement of broodstock and seed production technology. Information from this review can be useful in developing a better quality of crustacean broodstock’s diet for commercial hatchery production. PMID:26981008

Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program, 2008 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoffnagle, Timothy L.; Hair, Donald; Gee, Sally

2009-03-31

The Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program is designed to rapidly increase numbers of Chinook salmon in stocks that are in imminent danger of extirpation in Catherine Creek (CC), Lostine River (LR) and upper Grande Ronde River (GR). Natural parr are captured and reared to adulthood in captivity, spawned (within stocks) and their progeny reared to smoltification before being released into the natal stream of their parents. This program is co-managed by ODFW, National Marine Fisheries Service, Nez Perce Tribe and Confederated Tribes of the Umatilla Indian Reservation. Presmolt rearing was initially conducted at Lookingglass Fish Hatcherymore » (LFH) but parr collected in 2003 and later were reared at Wallowa Fish Hatchery (WFH). Post-smolt rearing is conducted at Bonneville Fish Hatchery (BOH - freshwater) and at Manchester Research Station (MRS - saltwater). The CC and LR programs are being terminated, as these populations have achieved the goal of a consistent return of 150 naturally spawning adults, so the 2005 brood year was the last brood year collected for theses populations. The Grande Ronde River program continued with 300 fish collected each year. Currently, we are attempting to collect 150 natural parr and incorporate 150 parr collected as eggs from females with low ELISA levels from the upper Grande Ronde River Conventional Hatchery Program. This is part of a comparison of two methods of obtaining fish for a captive broodstock program: natural fish vs. those spawned in captivity. In August 2007, we collected 152 parr (BY 2006) from the upper Grande Ronde River and also have 155 Grande Ronde River parr (BY 2006) that were hatched from eyed eggs at LFH. During 2008, we were unable to collect natural parr from the upper Grande Ronde River. Therefore, we obtained 300 fish from low ELISA females from the upper Grande Ronde River Conventional Program. In October 2008 we obtained 170 eyed eggs from the upper Grande Ronde river Conventional Hatchery Program. We will attempt to collect natural parr in August 2009. This year 752 fish were removed from the captive population: 629 fish survived to gamete production and 123 fish died from various causes prior to spawning. Growth of the Captive Broodstock fish was similar to previous years. The saltwater fish have grown more slowly than those reared in freshwater. A total of 720 fish were sorted as maturing and 629 (87.4%) of them survived to spawn. We collected gametes from 273 females and 350 males from the 2002-2006 brood years in 2008, using 111 spawning matrices and collected 474,187 green eggs (1,737 eggs/female). All ripe males were spawned and no semen was collected for cryo-preservation. Of the 474,187 eggs collected for the BY 2008 F1 generation, 448,373 (94.6%) survived to the eyed stage. 68,612 (15.3%) were culled from females with high ELISA OD values for BKD prevention. For BY 2007, we collected a total of 477,048 eggs from all three populations and 407,369 (85.4%) reached the eyed stage, while 95,024 eyed eggs (23.3%) were culled for BKD prevention. Eyed eggs were hatched at Lookingglass Fish Hatchery, producing 267,131 fry. As parr, 153,371 fish were coded-wire tagged (CWT). For the 2006 F1 brood year, we collected 177,890 eggs and 149,073 (83.8%) reached the eyed stage. 83,826 eyed eggs (56.2%) were culled at the eyed stage for BKD prevention. 61,044 fry were produced (93.6%), 53,688 (88 %) survived to smolt. There were 54 bacterial kidney disease (BKD) mortalities at BOH and MRS, combined in this reporting period. Overall, there were fewer BKD mortalities in 2008 due to a reduced number of fish coming into the Captive Broodstock Program and a shift away from collecting wild parr to using eyed eggs from low ELISA females from the Conventional Hatchery Program. Unknown causes of death accounted for 32 deaths at MRS and BOH, combined in 2008. We continually examine and modify the operations of the Captive Broodstock Program to make improvements wherever possible. We continue to have difficulty with prevention and treatment of BKD outbreaks and continue to use erythromycin and azithromycin to treat this disease. We are also continuing to investigate other possible treatments and prophylactic measures. To reduce the incidence of BKD in offspring of the Captive Broodstock Program, we continue to allow culling of eyed eggs from females with high BKD ELISA values (generally >0.800 OD units but the cull level varies annually, depending on the distribution of ELISA values, number of eggs collected, and management considerations). We are also using ultrasound to determine maturity and sex of fish early in the maturation process and are now able to determine maturity and sex of most maturing fish in early April. This allows us to transfer maturing fish from saltwater to freshwater at a more natural time, which should improve fecundity and egg quality of saltwater-reared fish.« less

Status and prospects for the yellow perch genetic improvement program in Milwaukee, Wisconsin

USDA-ARS?s Scientific Manuscript database

In 2004, a broodstock improvement program was initiated with the aim of developing defined geographic strains of yellow perch that have been selected for improved growth. Following a survey of 19 populations ranging from the Mid-West to the Eastern coast of the United States, and subsequent efforts ...

Genetic diversity of wild and hatchery lake trout populations: Relevance for management and restoration in the Great Lakes

USGS Publications Warehouse

Page, K.S.; Scribner, K.T.; Burnham-Curtis, M.

2004-01-01

The biological diversity of lake trout Salvelinus namaycush in the upper Great Lakes was historically high, consisting of many recognizable morphological types and discrete spawning populations. During the 1950s and 1960s, lake trout populations were extirpated from much of the Great Lakes primarily as a result of overfishing and predation by the parasitic sea lamprey Petromyzon marinus. Investigations of how genetic diversity is partitioned among remnant wild lake trout populations and hatchery broodstocks have been advocated to guide lake trout management and conservation planning. Using microsatellite genetic markers, we estimated measures of genetic diversity and the apportionment of genetic variance among 6 hatchery broodstocks and 10 wild populations representing three morphotypes (lean, humper, and siscowet). Analyses revealed that different hatchery broodstocks and wild populations contributed disproportionally to the total levels of genetic diversity. The genetic affinities of hatchery lake trout reflected the lake basins of origin of the wild source populations. The variance in allele frequency over all sampled extant wild populations was apportioned primarily on the basis of morphotype (??MT = 0.029) and secondarily among geographically dispersed populations within each morphotype (??ST = 0.024). The findings suggest that the genetic divergence reflected in recognized morphotypes and the associated ecological and physiological specialization occurred prior to the partitioning of large proglacial lakes into the Great Lakes or as a consequence of higher contemporary levels of gene flow within than among morphotypes. Information on the relative contributions of different broodstocks to total gene diversity within the regional hatchery program can be used to prioritize the broodstocks to be retained and to guide future stocking strategies. The findings highlight the importance of ecological and phenotypic diversity in Great Lakes fish communities and emphasize that the management of wild remnant lake trout populations and the restoration of extirpated populations should recognize and make greater use of the genetic diversity that still exists.

QTL affecting stress response to crowding in a rainbow trout broodstock population

USDA-ARS?s Scientific Manuscript database

Background Genomic analyses have the potential to impact selective breeding programs by identifying markers that serve as proxies for traits which are expensive or difficult to measure. Also, identifying genes affecting traits of interest enhances our understanding of their underlying biochemical ...

Genetic assessment of a summer chum salmon metapopulation in recovery

PubMed Central

Small, Maureen P; Johnson, Thom H; Bowman, Cherril; Martinez, Edith

2014-01-01

Programs to rebuild imperiled wild fish populations often include hatchery-born fish derived from wild populations to supplement natural spawner abundance. These programs require monitoring to determine their demographic, biological, and genetic effects. In 1990s in Washington State, the Summer Chum Salmon Conservation Initiative developed a recovery program for the threatened Hood Canal summer chum salmon Evolutionarily Significant Unit (ESU) (the metapopulation) that used in-river spawners (wild fish) for each respective supplementation broodstock in six tributaries. Returning spawners (wild-born and hatchery-born) composed subsequent broodstocks, and tributary-specific supplementation was limited to three generations. We assessed impacts of the programs on neutral genetic diversity in this metapopulation using 16 microsatellite loci and a thirty-year dataset spanning before and after supplementation, roughly eight generations. Following supplementation, differentiation among subpopulations decreased (but not significantly) and isolation by distance patterns remained unchanged. There was no decline in genetic diversity in wild-born fish, but hatchery-born fish sampled in the same spawning areas had significantly lower genetic diversity and unequal family representation. Despite potential for negative effects from supplementation programs, few were detected in wild-born fish. We hypothesize that chum salmon natural history makes them less vulnerable to negative impacts from hatchery supplementation. PMID:24567747

Effective size of a wild salmonid population is greatly reduced by hatchery supplementation

PubMed Central

Christie, M R; Marine, M L; French, R A; Waples, R S; Blouin, M S

2012-01-01

Many declining and commercially important populations are supplemented with captive-born individuals that are intentionally released into the wild. These supplementation programs often create large numbers of offspring from relatively few breeding adults, which can have substantial population-level effects. We examined the genetic effects of supplementation on a wild population of steelhead (Oncorhynchus mykiss) from the Hood River, Oregon, by matching 12 run-years of hatchery steelhead back to their broodstock parents. We show that the effective number of breeders producing the hatchery fish (broodstock parents; Nb) was quite small (harmonic mean Nb=25 fish per brood-year vs 373 for wild fish), and was exacerbated by a high variance in broodstock reproductive success among individuals within years. The low Nb caused hatchery fish to have decreased allelic richness, increased average relatedness, more loci in linkage disequilibrium and substantial levels of genetic drift in comparison with their wild-born counterparts. We also documented a substantial Ryman–Laikre effect whereby the additional hatchery fish doubled the total number of adult fish on the spawning grounds each year, but cut the effective population size of the total population (wild and hatchery fish combined) by nearly two-thirds. We further demonstrate that the Ryman–Laikre effect is most severe in this population when (1) >10% of fish allowed onto spawning grounds are from hatcheries and (2) the hatchery fish have high reproductive success in the wild. These results emphasize the trade-offs that arise when supplementation programs attempt to balance disparate goals (increasing production while maintaining genetic diversity and fitness). PMID:22805657

Survival of vaccinated,feed-trained largemouth bass fry (Micropterus Salmoides Floridanus) during natural exposure to Flavobacterium columnare

USDA-ARS?s Scientific Manuscript database

Largemouth bass (Micropterus salmoides floridanus; Centrarchidae) are often reared in government hatchery programs, then stocked to supplement wild fish populations. After the eggs obtained from broodstock are hatched, fry are stocked into ponds to feed on zooplankton and other small invertebrates....

Genetic and demographic implications of aquaculture in white sturgeon (Acipenser transmontanus)conservation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jager, Yetta

2005-01-01

This study uses a genetic individual-based model of white sturgeon (Acipenser transmontanus) populations in a river to examine the genetic and demographic trade-offs associated with operating a conservation hatchery. Simulation experiments evaluated three management practices: (i) setting quotas to equalize family contributions in an effort to prevent genetic swamping, (ii) an adaptive management scheme that interrupts stocking when introgression exceeds a specified threshold, and (iii) alternative broodstock selection strategies that influence domestication. The first set of simulations, designed to evaluate equalizing the genetic contribution of families, did not show the genetic benefits expected. The second set of simulations showed thatmore » simulated adaptive management was not successful in controlling introgression over the long term, especially with uncertain feedback. The third set of simulations compared the effects of three alternative broodstock selection strategies on domestication for hypothetical traits controlling early density-dependent survival. Simulated aquaculture selected for a density-tolerant phenotype when broodstock were taken from a genetically connected population. Using broodstock from an isolated population (i.e., above an upstream barrier or in a different watershed) was more effective at preventing domestication than using wild broodstock from a connected population.« less

Hybrid striped bass national breeding program: research towards genetic improvement of a non-model species

USDA-ARS?s Scientific Manuscript database

The hybrid striped bass (HSB) farming industry at present relies almost totally on wild broodstock for annual production of larvae and fingerlings, and industry efforts to domesticate the parent species of the HSB (white bass: WB, Morone chrysops; striped bass: SB, M. saxatilis) have been fairly lim...

Hybrid striped bass National Breeding Program: Research towards genetic improvement of a non-model species

USDA-ARS?s Scientific Manuscript database

The hybrid striped bass (HSB) farming industry at present relies almost totally on wild broodstock for annual production of larvae and fingerlings, and industry efforts to domesticate the parent species of the HSB (white bass: WB, Morone chrysops; striped bass: SB, M. saxatilis) have been fairly lim...

Research on Captive Broodstock Programs for Pacific Salmon, 2001-2002 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry; Tezak, E.; Endicott, Rick

The efficacy of captive broodstock programs depends on high in-culture survival and the fitness of cultured salmon after release, either as adults or juveniles. Continuing captive broodstock research designed to improve technology is being conducted to cover all major life history stages of Pacific salmon. The following summarizes some of the work performed and results from the FY 2001 performance period: (1) The incidence of male maturation of age-1 chinook salmon was significantly reduced by reducing growth in the first year of rearing. (2) Experimentally manipulated growth rates of captively-reared coho salmon had significant effects on female maturation rate, eggmore » size, and fecundity, and the effects were stage-specific (i.e., pre-smolt vs. post-smolt). (3) A combination of Renogen and MT239 vaccination of yearling chinook salmon given an acute R. salmoninarum challenge had a significantly longer survival time than the mock-vaccinated group. The survival time was marginally higher than was seen in acutely challenged fish vaccinated with either Renogen or MT239 alone and suggests that a combination vaccine of Renogen and MT239 may be useful as both a prophylactic and therapeutic agent against BKD. (4) Full-sib (inbred) groups of chinook salmon have thus far exhibited lower ocean survival than half-sib and non-related groups. Effects of inbreeding on fluctuating asymmetry did not follow expected patterns. (5) Sockeye salmon were exposed to specific odorants at either the alevin/emergent fry stage or the smolt stage to determine the relative importance of odorant exposure during key developmental periods and the importance of exposure duration. (6) Experimental studies to determine the effects of exercise conditioning on steelhead reproductive behavior and the effects of male body size on chinook salmon fertilization success during natural spawning were completed.« less

Surveillance for nervous necrosis virus-specific antibodies in barramundi Lates calcarifer in Australian hatcheries.

PubMed

Jaramillo, Diana; Hick, Paul; Dyrting, Kitman; Anderson, Ian; Whittington, Richard J

2017-03-30

We conducted single point-in-time and repeated cross-sectional studies of the prevalence of antibodies against nervous necrosis virus (NNV) in populations of adult barramundi Lates calcarifer in Australia. Serum samples collected between 2002 and 2012 were analyzed with indirect ELISA (n = 468). Most of the samples were sourced from broodstock with unknown exposure history, and these were compared with reference populations with confirmed history of exposure to NNV. Non-lethally collected gonad fluid samples from economically valuable barramundi broodstock (n = 164) were tested for the presence of NNV using RT-quantitative PCR at the time of blood sampling to compare infectivity with serostatus, but no virus was detected. NNV-specific immunoreactivity in broodstock was significantly lower than that for immunized and persistently infected populations. Seroprevalence increased over time in broodstock sampled longitudinally, probably reflecting repeated exposure to NNV in a region where the virus was endemic. The seroprevalence for the broodstock was 23.8% over the entire sample period while a cross-sectional survey conducted in 2012 found a seroprevalence of 34.5% with no significant difference between populations based on the geographic region or the history of occurrence of viral nervous necrosis (VNN) disease in the progeny in the respective hatcheries. Although serological surveillance was useful for studying the history of exposure of barramundi to NNV, the lack of association between serostatus in broodstock and the subsequent occurrence of VNN disease in their progeny indicates that ELISA tests for anti-NNV antibodies are not suitable for the purpose of preventing vertical transmission of NNV in barramundi.

Genetic variation assessed with microsatellites in mass selection lines of the Pacific oyster ( Crassostrea gigas) in China

NASA Astrophysics Data System (ADS)

Wang, Xubo; Li, Qi; Yu, Hong; Kong, Lingfeng

2016-12-01

Four successive mass selection lines of the Pacific oyster, Crassostrea gigas, selected for faster growth in breeding programs in China were examined at ten polymorphic microsatellite loci to assess the level of allelic diversity and estimate the effective population size. These data were compared with those of their base population. The results showed that the genetic variation of the four generations were maintained at high levels with an average allelic richness of 18.8-20.6, and a mean expected heterozygosity of 0.902-0.921. They were not reduced compared with those of their base population. Estimated effective population sizes based on temporal variances in microsatellite frequencies were smaller to that of sex ratio-corrected broodstock count estimates. Using a relatively large number of broodstock and keeping an equal sex ratio in the broodstock each generation may have contributed to retaining the original genetic diversity and maintaining relatively large effective population size. The results obtained in this study showed that the genetic variation was not affected greatly by mass selection progress and high genetic variation still existed in the mass selection lines, suggesting that there is still potential for increasing the gains in future generations of C. gigas. The present study provided important information for future genetic improvement by selective breeding, and for the design of suitable management guidelines for genetic breeding of C. gigas.

Pathology Associated with White Spot Virus (WSV) Infection in Wild Broodstock of Tiger Prawns (Penaeus monodon)

PubMed Central

Kua, Beng Chu; Rashid, Noraziah Mat

2012-01-01

A total of six wild broodstocks of tiger prawns, Penaeus monodon, were found positive for White Spot Virus (WSV) with an IQ2000 detection kit. Using histopathology, the intranuclear inclusion of haemocyte due to WSV infection was observed in the epithelium cells of the antennal gland, stomach and gills. This result confirmed that the wild broodstocks were positive with WSV without showing any white spot. Additionally, histopathological examination also revealed an accumulation of haemocytes around the hepatopancreatic tubules resulting from bacterial infection. Encapsulation and nodule formation, as well as related necrosis, were also observed around the hepatopancreatic tubules infected with a metazoan parasite. Encysted tylocephalum larval cestodes were observed in the hepatopancreas, with haemocytic aggregation being observed around the infected tubules. These findings showed some bacterial and parasitic infections which, in addition to the viral infection itself, could contribute to the 80% mortality rate in wild broodstocks infected with WSV. PMID:24575228

Streptococcal Arthritis, Osteolysis, Myositis, and Spinal Meningitis in Channel Catfish Ictalurus punctatus Broodstock

USDA-ARS?s Scientific Manuscript database

This report details findings of an investigation into complaints by commercial fingerling producers of low-grade mortalities, poor reproductive success, emaciation, skin lesions, and severely arched backs among broodstock of channel catfish Ictalurus punctatus. Gross lesions involved the jaw, fin ba...

Development of yellow perch (Perca flavescens) broodstocks: initial characterization of growth and quality traits following grow-out of different stocks

USDA-ARS?s Scientific Manuscript database

Broodstocks of yellow perch (Perca flavescens) were initiated from fertilized gametes obtained from wild fish taken from the Perquimans River (North Carolina), Choptank River (Maryland), Lake Winnebago (Wisconsin), and Lac du Flambeau (Wisconsin). Populations at these sites were chosen based on the ...

Simulated growth and production of endangered Snake River Sockeye Salmon: Assessing management strategies for the nursery lakes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Luecke, C.; Wurtsbaugh, W.A.; Budy, P.

1996-06-01

This document examines the potential of employing a series of lake management strategies to enhance production of endangered Snake River sockeye salmon (Oncorhynchus nerka) in its historical nursery lakes in central Idaho. A combination of limnological sampling, experimentation, and simulation modeling was used to assess effects of lake fertilization and kokanee reduction on growth and survival of juvenile sockeye salmon. Juvenile sockeye salmon from a broodstock of this endangered species are being introduced into the lakes from 1995 to 1998. Results of our analyses indicated that several lakes were suitable for receiving broodstock progeny. Field experimentation and simulation modeling indicatedmore » that lake fertilization, coupled with a program of kokanee reduction, provided the management option most likely to enhance the survival of stocked juvenile sockeye salmon. Simulation models that encompass physiological requirements, ecological interactions, and life-history consequences could be used as templates to help develop recovery plans for other endangered fishes. 4 figs., 2 tabs.« less

Grande Ronde Endemic Spring Chinook Salmon Supplementation Program: Facility Operation and Maintenance and Monitoring and Evaluation, 1999 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boe, Stephen J.; Lofy, Peter T.

2002-11-01

This is the second annual report of a multi-year, multi-agency project to restore spring chinook salmon populations in the Grande Ronde River Basin (Grande Ronde Endemic Chinook Salmon Program--GRESCP). The Confederated Tribes of the Umatilla Indian Reservation (CTUIR) operates adult collection and juvenile acclimation facilities on Catherine Creek and the upper Grande Ronde River for Snake River spring chinook salmon. These two streams have historically supported populations that provided significant tribal and non-tribal fisheries. Supplementation using conventional and captive broodstock techniques is being used to increase natural production and restore fisheries in these two streams. Statement of Work Objectives formore » 1999: (1) Participate in development and continued implementation of the comprehensive multi year operations plan for the Grande Ronde Endemic Supplementation Program. (2) Ensure proper construction and trial operation of semi-permanent adult and juvenile facilities for use in 2000. (3) Monitor adult endemic spring chinook salmon populations and collect broodstock. (4) Plan detailed Monitoring and Evaluation for future years. (5) Monitor population abundance and characteristics and local environmental factors that may influence abundance and run timing of Grande Ronde River spring chinook populations. (6) Participate in Monitoring and Evaluation of the captive brood component of the Program to assure this component is contributing to the Program. (7) Participate in data collection for incidentally-caught bull trout and summer steelhead and planning for recovery of summer steelhead populations. (8) Document accomplishments and needs to permitters, comanagers, and funding agencies. (9) Communicate project results to the scientific community.« less

Genetic adaptation to captivity can occur in a single generation

PubMed Central

Christie, Mark R.; Marine, Melanie L.; French, Rod A.; Blouin, Michael S.

2012-01-01

Captive breeding programs are widely used for the conservation and restoration of threatened and endangered species. Nevertheless, captive-born individuals frequently have reduced fitness when reintroduced into the wild. The mechanism for these fitness declines has remained elusive, but hypotheses include environmental effects of captive rearing, inbreeding among close relatives, relaxed natural selection, and unintentional domestication selection (adaptation to captivity). We used a multigenerational pedigree analysis to demonstrate that domestication selection can explain the precipitous decline in fitness observed in hatchery steelhead released into the Hood River in Oregon. After returning from the ocean, wild-born and first-generation hatchery fish were used as broodstock in the hatchery, and their offspring were released into the wild as smolts. First-generation hatchery fish had nearly double the lifetime reproductive success (measured as the number of returning adult offspring) when spawned in captivity compared with wild fish spawned under identical conditions, which is a clear demonstration of adaptation to captivity. We also documented a tradeoff among the wild-born broodstock: Those with the greatest fitness in a captive environment produced offspring that performed the worst in the wild. Specifically, captive-born individuals with five (the median) or more returning siblings (i.e., offspring of successful broodstock) averaged 0.62 returning offspring in the wild, whereas captive-born individuals with less than five siblings averaged 2.05 returning offspring in the wild. These results demonstrate that a single generation in captivity can result in a substantial response to selection on traits that are beneficial in captivity but severely maladaptive in the wild. PMID:22184236

Nutrient effects of broodstocks on the larvae in Patinopecten yessoensis

NASA Astrophysics Data System (ADS)

Bai, Yucen; Zhang, Tao; Qiu, Tianlong; Gao, Yan; Zhang, Xiaofang

2015-07-01

Patinopecten yessoensis is a commercial valuable species. This study deals with the effect of nutrient effects of the broodstock (mainly ovaries) on the larvae. Concentrations of total carbohydrate, total protein and total lipid in the gonads of P. yessoensis from three Hatcheries (Hatchery 1, Hatchery 2, and Hatchery 3) were determined before and after spawning. The relationship between the nutrient concentration in ovaries before spawning (BC) and that of larvae (LC) was assessed as well as the change in nutrient levels in ovaries after spawning (DC). Results indicate that the BC of total carbohydrate (7.66%) and total lipid (14.48%) in ovaries were significantly higher than in testes (5.20%, 5.20% respectively), whereas the BC of total protein in the ovaries was lower (61.76%) than in the testes (81.67%). The different gonadal composition suggests the different nutrient demands between male and female broodstocks in breeding season. Patinopecten yessoensis gonads contained a higher proportion of lipids, in comparison to other bivalves, which might be a response to the low ambient water temperatures. Further analysis of fatty acids showed that the concentrations of n-3PUFA, EPA and DHA in larvae (LC) were positively correlated with BC and DC, indicating the significant nutrient influence of broodstocks on the larvae. As these fatty acids are important in metabolism, and have been demonstrated to be influential to the viability of the larvae, larval growth and the settlement, spat growth, and juvenile survival in many bivalves, they could possibly be used as indexes to evaluate, and predict condition of broodstocks and larvae.

Implementation of genetic conservation practices in a muskellunge propagation and stocking program

USGS Publications Warehouse

Jennings, Martin J.; Sloss, Brian L.; Hatzenbeler, Gene R.; Kampa, Jeffrey M.; Simonson, Timothy D.; Avelallemant, Steven P.; Lindenberger, Gary A.; Underwood, Bruce D.

2010-01-01

Conservation of genetic resources is a challenging issue for agencies managing popular sport fishes. To address the ongoing potential for genetic risks, we developed a comprehensive set of recommendations to conserve genetic diversity of muskellunge (Esox masquinongy) in Wisconsin, and evaluated the extent to which the recommendations can be implemented. Although some details are specific to Wisconsin's muskellunge propagation program, many of the practical issues affecting implementation are applicable to other species and production systems. We developed guidelines to restrict future broodstock collection operations to lakes with natural reproduction and to develop a set of brood lakes to use on a rotational basis within regional stock boundaries, but implementation will require considering lakes with variable stocking histories. Maintaining an effective population size sufficient to minimize the risk of losing alleles requires limiting broodstock collection to large lakes. Recommendations to better approximate the temporal distribution of spawning in hatchery operations and randomize selection of brood fish are feasible. Guidelines to modify rearing and distribution procedures face some logistic constraints. An evaluation of genetic diversity of hatchery-produced fish during 2008 demonstrated variable success representing genetic variation of the source population. Continued evaluation of hatchery operations will optimize operational efficiency while moving toward genetic conservation goals.

Research on Captive Broodstock Programs for Pacific Salmon; Assessment of Captive Broodstock Technologies, Annual Report 2002-2003.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry

The success of captive broodstock programs depends on high in-culture survival, appropriate development of the reproductive system, and the behavior and survival of cultured salmon after release, either as adults or juveniles. Continuing captive broodstock research designed to improve technology is being conducted to cover all major life history stages of Pacific salmon. Current velocity in rearing vessels had little if any effect on reproductive behavior of captively reared steelhead. However, males and females reared in high velocity vessels participated a greater number of spawning events than siblings reared in low velocity tanks. Observations of nesting females and associated malesmore » in a natural stream (Hamma Hamma River) were consistent with those observed in a controlled spawning channel. DNA pedigree analyses did not reveal significant differences in the numbers of fry produced by steelhead reared in high and low velocity vessels. To determine the critical period(s) for imprinting for sockeye salmon, juvenile salmon are being exposed to known odorants at key developmental stages. Subsequently they will be tested for development of long-term memories of these odorants. In 2002-2003, the efficacy of EOG analysis for assessing imprinting was demonstrated and will be applied in these and other behavioral and molecular tools in the current work plan. Results of these experiments will be important to determine the critical periods for imprinting for the offspring of captively-reared fish destined for release into natal rivers or lakes. By early August, the oocytes of all of Rapid River Hatchery chinook salmon females returning from the ocean had advanced to the tertiary yolk globule stage; whereas, only some of the captively reared Lemhi River females sampled had advanced to this stage, and the degree of advancement was not dependent on rearing temperature. The mean spawning time of captive Lemhi River females was 3-4 weeks after that of the Rapid River fish. Captive Lemhi River females produced smaller and fewer eggs than the Rapid River females; however, relative fecundity was higher than that of the Rapid River fish. Female coho salmon that ceased or slowed oocyte development in the spring had lower body growth from the previous August onward compared with females that continued oocyte growth. This indicates that growth during the late summer and fall, one year prior to spawning, can determine the decision to mature the following spring. Therefore it is important to maintain the growth of broodstock during the summer/fall period to ensure the continuation of ovary development in the subsequent spring. A combined whole cell vaccine of Renogen with killed R. salmoninarum strain MT239 may be effective in reducing the occurrence of BKD during the period immediately after seawater transfer, but not in yearling seawater-adapted chinook salmon. Control of BKD is likely to require an integrated disease management plan, utilizing three components, namely broodstock segregation, antibiotics, and vaccination. Vaccine results incorporated with antibiotic treatment will be used to work toward an integrated disease management plan to help to reduce the cycle of BKD transmission in the captive stocks to increase survival safely. Patterns of estimated survival in one chinook salmon stock (Grovers Creek) were generally consistent with inbreeding depression: progeny of fish that were full siblings (approximate increment in F of 0.25) survived to return at much lower rates than did progeny of fish that were half siblings (F {approx} 0.125) or unrelated individuals (F {approx} 0). Growth at sea of Grovers Creek Hatchery stock study fish was lower than that of UWH stock fish. Among the inbreeding groups alone, no clear differences in growth were detectable. However, preliminary results suggest the general pattern of growth was opposite that expected if inbreeding depression reduced growth: the highest growth was in progeny of related parents.« less

Grande Ronde Endemic Spring Chinook Salmon Supplementation Program: Facility Operation and Maintenance and Monitoring and Evaluation, 2000 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boe, Stephen J.; Lofy, Peter T.

2003-03-01

This is the third annual report of a multi-year project to operate adult collection and juvenile acclimation facilities on Catherine Creek and the upper Grande Ronde River for Snake River spring chinook salmon. These two streams have historically supported populations that provided significant tribal and non-tribal fisheries. Supplementation using conventional and captive broodstock techniques is being used to restore fisheries in these streams. Statement of Work Objectives for 2000: (1) Participate in implementation of the comprehensive multiyear operations plan for the Grande Ronde Endemic Spring Chinook Supplementation Program (GRESCP). (2) Plan for recovery of endemic summer steelhead populations in Catherinemore » Creek and the upper Grande Ronde River. (3) Ensure proper construction and trial operation of semi-permanent adult and juvenile facilities for use in 2000. (4) Collect summer steelhead. (5) Collect adult endemic spring chinook salmon broodstock. (6) Acclimate juvenile spring chinook salmon prior to release into the upper Grande Ronde River and Catherine Creek. (7) Document accomplishments and needs to permitters, comanagers, and funding agency. (8) Communicate project results to the scientific community. (9) Plan detailed GRESCP Monitoring and Evaluation for future years. (10) Monitor adult population abundance and characteristics of Grande Ronde River spring chinook salmon populations and incidentally-caught summer steelhead and bull trout. (11) Monitor condition, movement, and mortality of spring chinook salmon acclimated at remote facilities. (12) Monitor water quality at facilities. (13) Participate in Monitoring & Evaluation of the captive brood component of the Program to document contribution to the Program.« less

Grande Ronde Endemic Spring Chinook Salmon Supplementation Program: Facility Operation and Maintenance and Monitoring and Evaluation, 2001 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boe, Stephen J.; Ogburn, Parker N.

2003-03-01

This is the second annual report of a multi-year project to operate adult collection and juvenile acclimation facilities on Catherine Creek and the upper Grande Ronde River for Snake River spring chinook salmon. These two streams have historically supported populations that provided significant tribal and non-tribal fisheries. Supplementation using conventional and captive broodstock techniques is being used to restore fisheries in these streams. Statement of Work Objectives for 2001: (1) Participate in implementation of the comprehensive multiyear operations plan for the Grande Ronde Endemic Spring chinook Supplementation Program (GRESCP). (2) Plan detailed GRESCP Monitoring and Evaluation for future years. (3)more » Ensure proper construction and trial operation of semi-permanent adult and juvenile facilities for use in 2001. (4) Plan for data collection needs for bull trout. (5) Ensure proper construction and trial operation of semi-permanent adult and juvenile facilities for use in 2001. (6) Collect summer steelhead. (7) Monitor adult endemic spring chinook salmon populations and collect broodstock. (8) Acclimate juvenile spring chinook salmon prior to release into the upper Grande Ronde River and Catherine Creek. (9) Monitor adult population abundance and characteristics of Grande Ronde River spring chinook salmon populations. (10) Monitor condition, movement, and mortality of spring chinook salmon acclimated at remote facilities. (11) Participate in Monitoring & Evaluation of the captive brood component of the Program to document contribution to the Program. (12) Monitor water quality at facilities. (13) Document accomplishments and needs to permitters, comanagers, and funding agencies. (14) Communicate Project results to the scientific community.« less

Broodstock History Strongly Influences Natural Spawning Success in Hatchery Steelhead (Oncorhynchus mykiss).

PubMed

Ford, Michael J; Murdoch, Andrew R; Hughes, Michael S; Seamons, Todd R; LaHood, Eric S

2016-01-01

We used genetic parentage analysis of 6200 potential parents and 5497 juvenile offspring to evaluate the relative reproductive success of hatchery and natural steelhead (Onchorhynchus mykiss) when spawning in the wild between 2008 and 2011 in the Wenatchee River, Washington. Hatchery fish originating from two prior generation hatchery parents had <20% of the reproductive success of natural origin spawners. In contrast, hatchery females originating from a cross between two natural origin parents of the prior generation had equivalent or better reproductive success than natural origin females. Males originating from such a cross had reproductive success of 26-93% that of natural males. The reproductive success of hatchery females and males from crosses consisting of one natural origin fish and one hatchery origin fish was 24-54% that of natural fish. The strong influence of hatchery broodstock origin on reproductive success confirms similar results from a previous study of a different population of the same species and suggests a genetic basis for the low reproductive success of hatchery steelhead, although environmental factors cannot be entirely ruled out. In addition to broodstock origin, fish size, return time, age, and spawning location were significant predictors of reproductive success. Our results indicate that incorporating natural fish into hatchery broodstock is clearly beneficial for improving subsequent natural spawning success, even in a population that has a decades-long history of hatchery releases, as is the case in the Wenatchee River.

Grande Ronde Endemic Spring Chinook Salmon Supplementation Program: Monitoring and Evaluation, 2002 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boe, Stephen J.; Weldert, Rey F.; Crump, Carrie A.

2003-03-01

This is the fifth annual report of a multi-year project to operate adult collection and juvenile acclimation facilities on Catherine Creek and the upper Grande Ronde River for Snake River spring chinook salmon. These two streams have historically supported populations that provided significant tribal and non-tribal fisheries. Conventional and captive broodstock supplementation techniques are being used to restore spring chinook salmon fisheries in these streams. Statement of Work Objectives for 2002: (1) Plan for, administer, coordinate and assist comanagers in GRESCP M&E activities. (2) Evaluate performance of supplemented juvenile spring chinook salmon. (3) Evaluate life history differences between wild andmore » hatchery-origin (F{sub 1}) adult spring chinook salmon. (4) Describe life history characteristics and genetics of adult summer steelhead collected at weirs.« less

Snake River Sockeye Salmon Captive Broodstock Program Research Elements : 2007 Annual Project Progess Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peterson, Mike; Plaster, Kurtis; Redfield, Laura

2008-12-17

On November 20, 1991, the National Oceanic Atmospheric Administration listed Snake River sockeye salmon Oncorhynchus nerka as endangered under the Endangered Species Act of 1973. In 1991, the Shoshone-Bannock Tribes (SBT) and Idaho Department of Fish and Game (IDFG) initiated the Snake River Sockeye Salmon Captive Broodstock Program to conserve and rebuild populations in Idaho. Restoration efforts are focused on Redfish, Pettit, and Alturas lakes within the Sawtooth Valley. The first release of hatchery-produced adults occurred in 1993. The first release of juvenile sockeye salmon from the captive broodstock program occurred in 1994. In 1999, the first anadromous adult returnsmore » from the captive broodstock program were recorded when six jacks and one jill were captured at the IDFG Sawtooth Fish Hatchery. In 2007, progeny from the captive broodstock program were released using four strategies: (1) eyed-eggs were planted in Pettit Lake in November; (2) age-0 presmolts were released to Alturas, Pettit, and Redfish lakes in October; (3) age-1 smolts were released into Redfish Lake Creek and the upper Salmon River in May; and (4) hatchery-produced adult sockeye salmon were released to Redfish Lake for volitional spawning in September. Oncorhynchus nerka population monitoring was conducted on Redfish, Alturas, and Pettit lakes using a midwater trawl in September 2007. Population abundances were estimated at 73,702 fish for Redfish Lake, 124,073 fish for Alturas Lake, and 14,746 fish for Pettit Lake. Angler surveys were conducted from May 26 through August 7, 2007 on Redfish Lake to estimate kokanee harvest. On Redfish Lake, we interviewed 102 anglers and estimated that 56 kokanee were harvested. The calculated kokanee catch rate was 0.03 fish/hour for each kokanee kept. The juvenile out-migrant trap on Redfish Lake Creek was operated from April 14 to June 13, 2007. We estimated that 5,280 natural origin and 14,256 hatchery origin sockeye salmon smolts out-migrated from Redfish Lake in 2007. The hatchery origin component originated from a 2006 fall presmolt direct-release. The juvenile out-migrant traps on Alturas Lake Creek and Pettit Lake Creek were operated by the SBT from April 19 to May 23, 2007 and April 18 to May 29, 2007, respectively. The SBT estimated 1,749 natural origin and 4,695 hatchery origin sockeye salmon smolts out-migrated from Pettit Lake and estimated 8,994 natural origin and 6,897 hatchery origin sockeye salmon smolts out-migrated from Alturas Lake in 2007. The hatchery origin component of sockeye salmon out-migrants originated from fall presmolt direct-releases made to Pettit and Alturas lakes in 2006. In 2007, the Stanley Basin Sockeye Technical Oversight Committee (SBSTOC) chose to have all Snake River sockeye salmon juveniles (tagged and untagged) transported due to potential enhanced survival. Therefore, mainstem survival evaluations were only conducted to Lower Granite Dam. Unique PIT tag interrogations from Sawtooth Valley juvenile out-migrant traps to Lower Granite Dam were utilized to estimate survival rates for out-migrating sockeye salmon smolts. Survival rate comparisons were made between smolts originating from Redfish, Alturas, and Pettit lakes and the various release strategies. Alturas Lake hatchery origin smolts tagged at the out-migrant trap recorded the highest survival rate of 78.0%. In 2007, 494 hatchery origin adult sockeye salmon were released to Redfish Lake for natural spawning. We observed 195 areas of excavation in the lake from spawning events. This was the highest number of redds observed in Redfish Lake since the program was initiated. Suspected redds were approximately 3 m x 3 m in size and were constructed by multiple pairs of adults. To monitor the predator population found within the lakes, we monitored bull trout spawning in Fishhook Creek, a tributary to Redfish Lake; and in Alpine Creek, a tributary to Alturas Lake. This represented the tenth consecutive year that the index reaches have been surveyed on these two streams. Adult counts (41 adults) and redd counts (22 redds) in Fishhook Creek increased compared to counts conducted since monitoring began in 1998. Beginning in 2007, we also surveyed an additional trend site in Fishhook Creek resulting in observing 43 adult bull trout and 30 additional redds. Bull trout numbers (13 adults) and the number of redds observed (18 redds) have gradually increased in Alpine Creek compared to counts from initial monitoring.« less

Broodstock History Strongly Influences Natural Spawning Success in Hatchery Steelhead (Oncorhynchus mykiss)

PubMed Central

Murdoch, Andrew R.; Hughes, Michael S.; Seamons, Todd R.; LaHood, Eric S.

2016-01-01

We used genetic parentage analysis of 6200 potential parents and 5497 juvenile offspring to evaluate the relative reproductive success of hatchery and natural steelhead (Onchorhynchus mykiss) when spawning in the wild between 2008 and 2011 in the Wenatchee River, Washington. Hatchery fish originating from two prior generation hatchery parents had <20% of the reproductive success of natural origin spawners. In contrast, hatchery females originating from a cross between two natural origin parents of the prior generation had equivalent or better reproductive success than natural origin females. Males originating from such a cross had reproductive success of 26–93% that of natural males. The reproductive success of hatchery females and males from crosses consisting of one natural origin fish and one hatchery origin fish was 24–54% that of natural fish. The strong influence of hatchery broodstock origin on reproductive success confirms similar results from a previous study of a different population of the same species and suggests a genetic basis for the low reproductive success of hatchery steelhead, although environmental factors cannot be entirely ruled out. In addition to broodstock origin, fish size, return time, age, and spawning location were significant predictors of reproductive success. Our results indicate that incorporating natural fish into hatchery broodstock is clearly beneficial for improving subsequent natural spawning success, even in a population that has a decades-long history of hatchery releases, as is the case in the Wenatchee River. PMID:27737000

Salmon Supplementation Studies in Idaho Rivers; Field Activities Conducted on Clear and Pete King Creeks, 2002 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bretz, Justin K.; Olson, Jill M.

2003-03-01

In 2002 the Idaho Fisheries Resource Office continued working as a cooperator on the Salmon Supplementation Studies in Idaho Rivers (ISS) project on Pete King and Clear creeks. Data relating to supplementation treatment releases, juvenile sampling, juvenile PIT tagging, broodstock spawning and rearing, spawning ground surveys, and snorkel surveys were used to evaluate the project data points and augment past data. Supplementation treatments included the release of 51,329 left ventral-clipped smolts into Clear Creek (750 were PIT tagged), and 12,000 unmarked coded-wire tagged parr into Pete King Creek (998 were PIT tagged). Using juvenile collection methods, Idaho Fisheries Resource Officemore » staff PIT tagged and released 579 naturally produced spring chinook juveniles in Clear Creek, and 54 on Pete King Creek, for minimum survival estimates to Lower Granite Dam. For Clear Creek, minimum survival estimates to Lower Granite Dam of hatchery produced supplementation and naturally produced PIT tagged smolts, were 36.0%, and 53.1%, respectively. For Pete King Creek, minimum survival estimates to Lower Granite Dam, of hatchery produced supplementation smolts and naturally produced smolts PIT tagged as parr and presmolts, were 18.8%, and 8.3%, respectively. Adults collected for broodstock in 2002 represented the final adult broodstock group collected for the ISS project. Twenty-six ventral clipped, and 28 natural adult spring chinook were transported above the weir. Monitoring and evaluation of spawning success was continued on Clear and Pete King creeks. A total of 69 redds were counted and 79 carcasses were recovered on Clear Creek. Two redds were observed and no carcasses were collected on Pete King Creek.« less

Research on Captive Broodstock Programs for Pacific Salmon, 2002-2003 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry A.

The success of captive broodstock programs depends on high in-culture survival, appropriate development of the reproductive system, and the behavior and survival of cultured salmon after release, either as adults or juveniles. Continuing captive broodstock research designed to improve technology is being conducted to cover all major life history stages of Pacific salmon. Current velocity in rearing vessels had little if any effect on reproductive behavior of captively reared steelhead. However, males and females reared in high velocity vessels participated a greater number of spawning events than siblings reared in low velocity tanks. Observations of nesting females and associated malesmore » in a natural stream (Hamma Hamma River) were consistent with those observed in a controlled spawning channel. DNA pedigree analyses did not reveal significant differences in the numbers of fry produced by steelhead reared in high and low velocity vessels. To determine the critical period(s) for imprinting for sockeye salmon, juvenile salmon are being exposed to known odorants at key developmental stages. Subsequently they will be tested for development of long-term memories of these odorants. In 2002-2003, the efficacy of EOG analysis for assessing imprinting was demonstrated and will be applied in these and other behavioral and molecular tools in the current work plan. Results of these experiments will be important to determine the critical periods for imprinting for the offspring of captively-reared fish destined for release into natal rivers or lakes. By early August, the oocytes of all of Rapid River Hatchery chinook salmon females returning from the ocean had advanced to the tertiary yolk globule stage; whereas, only some of the captively reared Lemhi River females sampled had advanced to this stage, and the degree of advancement was not dependent on rearing temperature. The mean spawning time of captive Lemhi River females was 3-4 weeks after that of the Rapid River fish. Captive Lemhi River females produced smaller and fewer eggs than the Rapid River females; however, relative fecundity was higher than that of the Rapid River fish. Female coho salmon that ceased or slowed oocyte development in the spring had lower body growth from the previous August onward compared with females that continued oocyte growth. This indicates that growth during the late summer and fall, one year prior to spawning, can determine the decision to mature the following spring. Therefore it is important to maintain the growth of broodstock during the summer/fall period to ensure the continuation of ovary development in the subsequent spring. A combined whole cell vaccine of Renogen with killed R. salmoninarum strain MT239 may be effective in reducing the occurrence of BKD during the period immediately after seawater transfer, but not in yearling seawater-adapted chinook salmon. Control of BKD is likely to require an integrated disease management plan, utilizing three components, namely broodstock segregation, antibiotics, and vaccination. Vaccine results incorporated with antibiotic treatment will be used to work toward an integrated disease management plan to help to reduce the cycle of BKD transmission in the captive stocks to increase survival safely. Patterns of estimated survival in one chinook salmon stock (Grovers Creek)were generally consistent with inbreeding depression: progeny of fish that were full siblings (approximate increment in F of 0.25) survived to return at much lower rates than did progeny of fish that were half siblings (F {approx} 0.125) or unrelated individuals (F {approx} 0). Growth at sea of Grovers Creek Hatchery stock study fish was lower than that of UWH stock fish. Among the inbreeding groups alone, no clear differences in growth were detectable. However, preliminary results suggest the general pattern of growth was opposite that expected if inbreeding depression reduced growth: the highest growth was in progeny of related parents.« less

How well can captive breeding programs conserve biodiversity? A review of salmonids

PubMed Central

Fraser, Dylan J

2008-01-01

Captive breeding programs are increasingly being initiated to prevent the imminent extinction of endangered species and/or populations. But how well can they conserve genetic diversity and fitness, or re-establish self-sustaining populations in the wild? A review of these complex questions and related issues in salmonid fishes reveals several insights and uncertainties. Most programs can maintain genetic diversity within populations over several generations, but available research suggests the loss of fitness in captivity can be rapid, its magnitude probably increasing with the duration in captivity. Over the long-term, there is likely tremendous variation between (i) programs in their capacity to maintain genetic diversity and fitness, and (ii) species or even intraspecific life-history types in both the severity and manner of fitness-costs accrued. Encouragingly, many new theoretical and methodological approaches now exist for current and future programs to potentially reduce these effects. Nevertheless, an unavoidable trade-off exists between conserving genetic diversity and fitness in certain instances, such as when captive-bred individuals are temporarily released into the wild. Owing to several confounding factors, there is also currently little evidence that captive-bred lines of salmonids can or cannot be reintroduced as self-sustaining populations. Most notably, the root causes of salmonid declines have not been mitigated where captive breeding programs exist. Little research has also addressed under what conditions an increase in population abundance due to captive-rearing might offset fitness reductions induced in captivity. Finally, more empirical investigation is needed to evaluate the genetic/fitness benefits and risks associated with (i) maintaining captive broodstocks as either single or multiple populations within one or more facilities, (ii) utilizing cryopreservation or surrogate broodstock technologies, and (iii) adopting other alternatives to captive-rearing such as translocations to new habitats. Management recommendations surrounding these issues are proposed, with the aim of facilitating meta-analyses and more general principles or guidelines for captive-breeding. These include the need for the following: (i) captive monitoring to involve, a priori, greater application of hypothesis testing through the use of well-designed experiments and (ii) improved documentation of procedures adopted by specific programs for reducing the loss of genetic diversity and fitness. PMID:25567798

Grande Ronde Endemic Spring Chinook Salmon Supplementation Program : Facility Operation and Maintenance Facilities, Annual Report 2003.

DOE Office of Scientific and Technical Information (OSTI.GOV)

McLean, Michael L.; Seeger, Ryan; Hewitt, Laurie

2004-01-01

Anadromous salmonid stocks have declined in both the Grande Ronde River Basin (Lower Snake River Compensation Plan (LSRCP) Status Review Symposium 1998) and in the entire Snake River Basin (Nehlsen et al. 1991), many to the point of extinction. The Grande Ronde River Basin historically supported large populations of fall and spring chinook (Oncorhynchus tshawytscha), sockeye (O. nerka), and coho (O. kisutch) salmon and steelhead trout (O. mykiss) (Nehlsen et al. 1991). The decline of chinook salmon and steelhead populations and extirpation of coho and sockeye salmon in the Grande Ronde River Basin was, in part, a result of constructionmore » and operation of hydroelectric facilities, over fishing, and loss and degradation of critical spawning and rearing habitat in the Columbia and Snake River basins (Nehlsen et al. 1991). Hatcheries were built in Oregon, Washington and Idaho under the Lower Snake River Compensation Plan (LSRCP) to compensate for losses of anadromous salmonids due to the construction and operation of the lower four Snake River dams. Lookingglass Hatchery (LGH) on Lookingglass Creek, a tributary of the Grande Ronde River, was completed under LSRCP in 1982 and has served as the main incubation and rearing site for chinook salmon programs for Grande Ronde and Imnaha rivers in Oregon. Despite these hatchery programs, natural spring chinook populations continued to decline resulting in the National Marine Fisheries Service (NMFS) listing Snake River spring/summer chinook salmon as ''threatened'' under the federal Endangered Species Act (1973) on 22 April 1992. Continuing poor escapement levels and declining population trends indicated that Grande Ronde River basin spring chinook salmon were in imminent danger of extinction. These continuing trends led fisheries co-managers in the basin to initiate the Grande Ronde Endemic Spring Chinook Salmon Supplementation Program (GRESCSSP) in order to prevent extinction and preserve options for use of endemic fish stocks in future artificial propagation programs. The GRESCSSP was implemented in three Grande Ronde River basin tributaries; the Lostine and upper Grande Ronde rivers and Catherine Creek. The GRESCSSP employs two broodstock strategies utilizing captive and conventional brood sources. The captive brood program began in 1995, with the collection of parr from the three tributary areas. The conventional broodstock component of the program began in 1997 with the collection of natural adults returning to these tributary areas. Although LGH was available as the primary production facility for spring chinook programs in the Grande Ronde Basin, there were never any adult or juvenile satellite facilities developed in the tributary areas that were to be supplemented. An essential part of the GRESCSSP was the construction of adult traps and juvenile acclimation facilities in these tributary areas. Weirs were installed in 1997 for the collection of adult broodstock for the conventional component of the program. Juvenile facilities were built in 2000 for acclimation of the smolts produced by the captive and conventional broodstock programs and as release sites within the natural production areas of their natal streams. The Confederated Tribes of the Umatilla Indian Reservation (CTUIR) operate both the juvenile acclimation and adult trapping facilities located on Catherine Creek and the upper Grande Ronde River under this project. The Nez Perce Tribe (NPT) operate the facilities on the Lostine River under a sister project. Hatcheries were also built in Oregon, Washington and Idaho under the LSRCP to compensate for losses of summer steelhead due to the construction and operation of the lowest four Snake River dams. Despite these harvest-driven hatchery programs, natural summer steelhead populations continued to decline as evidenced by declining counts at Lower Granite Dam since 1995 (Columbia River Data Access in Real Time, DART) and low steelhead redd counts on index streams in the Grande Ronde Basin. Because of low escapement the Snake River summer steelhead were listed as threatened under the Endangered Species Act of 1973 by the National Marine Fisheries Service (NMFS) on 18 August, 1997. Co-managers have also discontinued off-station releases of juvenile Wallowa stock (non-endemic) hatchery summer steelhead into Catherine Creek in 1998 and the upper Grande Ronde River in 1999. Data are lacking on adult return numbers and the genetic make-up of populations that return to tributaries of the Grande Ronde River basin, Catherine Creek and the upper Grande Ronde River specifically. Adult fish weirs are in place on Catherine Creek and the upper Grande Ronde River and data on summer steelhead populations in those areas are collected.« less

Migratory urge and gll Na+,K+-ATPase activity of hatchery-reared Atlantic salmon smolts from the Dennys and Penobscot River stocks, Maine

USGS Publications Warehouse

Spencer, Randall C.; Zydlewski, Joseph D.; Zydlewski, Gayle B.

2010-01-01

Hatchery-reared Atlantic salmon Salmo salar smolts produced from captive-reared Dennys River and sea-run Penobscot River broodstock are released into their source rivers in Maine. The adult return rate of Dennys smolts is comparatively low, and disparity in smolt quality between stocks resulting from genetic or broodstock rearing effects is plausible. Smolt behavior and physiology were assessed during sequential 14-d trials conducted in seminatural annular tanks with circular flow. “Migratory urge” (downstream movement) was monitored remotely using passive integrated transponder tags, and gill Na+,K+-ATPase activity was measured at the beginning and end of the trials to provide an index of smolt development. The migratory urge of both stocks was low in early April, increased 20-fold through late May, and declined by the end of June. The frequency and seasonal distribution of downstream movement were independent of stock. In March and April, initial gill Na+,K+-ATPase activities of Penobscot River smolts were lower than those of Dennys River smolts. For these trials, however, Penobscot River smolts increased enzyme activity after exposure to the tank, whereas Dennys River smolts did not, resulting in similar activities between stocks at the end of all trials. There was no clear relationship between migratory urge and gill Na+,K+-ATPase activity. Gill Na+,K+-ATPase activity of both stocks increased in advance of migratory urge and then declined while migratory urge was increasing. Maximum movement was observed from 2 h after sunset through 1 h after sunrise but varied seasonally. Dennys River smolts were slightly more nocturnal than Penobscot River smolts. These data suggest that Dennys and Penobscot River stocks are not markedly different in either physiological or behavioral expression of smolting.

"Research to Improve the Efficacy of Captive Broodstock Programs and Advance Hatchery Reform Throughout the Columbia River Basin." [from the Abstract], 2007-2008 Annual Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry A.

This project was developed to conduct research to improve the efficacy of captive broodstock programs and advance hatchery reform throughout the Columbia river basin. The project has three objectives: (1) maintain adaptive life history characteristics in Chinook salmon, (2) improve imprinting in juvenile sockeye salmon, and (3) match wild phenotypes in Chinook and sockeye salmon reared in hatcheries. A summary of the results are as follows: Objective 1: Adult and jack Chinook salmon males were stocked into four replicate spawning channels at a constant density (N = 16 per breeding group), but different ratios, and were left to spawn naturallymore » with a fixed number of females (N = 6 per breeding group). Adult males obtained primary access to females and were first to enter the nest at the time of spawning. Jack male spawning occurred primarily by establishing satellite positions downstream of the courting pair, and 'sneaking' into the nest at the time of spawning. Male dominance hierarchies were fairly stable and strongly correlated with the order of nest entry at the time of spawning. Spawning participation by jack and adult males is consistent with a negative frequency dependent selection model, which means that selection during spawning favors the rarer life history form. Results of DNA parentage assignments will be analyzed to estimate adult-to-fry fitness of each male. Objective 2: To determine the critical period(s) for imprinting for sockeye salmon, juvenile salmon were exposed to known odorants at key developmental stages. Molecular assessments of imprinting-induced changes in odorant receptor gene expression indicated that regulation of odorant expression is influenced by developmental status and odor exposure history. The results suggest that sockeye salmon are capable of imprinting to homing cues during the developmental periods that correspond to several of current release strategies employed as part of the Captive Broodstock program (specifically, planting eyed eggs, fall and smolt releases into the lake) appear to be appropriate for successful homing of sockeye in Redfish Lake. Also, our findings indicated that sockeye salmon were capable of olfactory imprinting at multiple life stages and over varying exposure durations. Fish exposed to odors just prior to smolting showed the strongest attraction to the imprinting odor arginine and this period corresponds to the period of highest plasma thyroxine levels and increased BAAR receptor mRNA in juveniles. Objective 3: Spring Chinook salmon were exposed to three different photoperiods and three feed rations at the button-up stage of development. Both photoperiod at emergence and ration post-ponding affected the number of males maturing at age one. Nearly 70% of the males in the early emergence and satiation fed group matured after the first year of rearing, while none of the fish reared on late emergence photoperiod (equivalent to emergence on May 1) matured during this time irrespective of ration treatment. Within the early emergence groups, reducing growth using ration (low or high) appeared to reduce the number of males maturing at age one from 70% to 40-50%. Maturation rates of fish that emerged in a photoperiod equivalent to mid-February (middle emergence) ranged from 10-25%. Together these data indicate that the seasonal timing of fry emergence and growth after ponding can alter life history patterns in spring Chinook salmon. The results imply that hatchery rearing practices that alter seasonal timing of fry emergence can have drastic effects on life history patterns in juvenile Chinook salmon. All three objectives are on-going and will result in recommendations (at the end of the FY 2009 performance period) to advance hatchery reforms in conventional and captive broodstock programs.« less

Effect of dietary marine lipids on female white bass ova compositions and progeny survival.

PubMed

Lewis, H A; Trushenski, J T; Lane, R L; Kohler, C C

2010-12-01

We evaluated white bass ovum fatty acid composition as well as embryonic and larval survival after varying n-3 and n-6 long-chain polyunsaturated fatty acid (LC-PUFA) concentrations in maternal diets. Diets containing graded levels (0, 33, 66, or 100%) of squid to menhaden oils were fed daily to apparent satiation to female white bass for 8 weeks prior to spawning. Embryonic survival was negatively related to maternal squid oil intake (P=0.015, R2=0.970). Squid oil-fed broodstock produced ova with decreased 20:5n-3 and increased C18 polyunsaturated fatty acid concentrations, largely reflecting the fatty acid profile of squid oil. Within ovum phospholipid, accumulation of 18:2n-6 may have altered biological function resulting in the lower embryonic survival among ova produced from the squid oil-fed broodstock. Our data suggest the importance of feeding white bass broodstock diets high in total n-3 LC-PUFA (at least 4.0% dry matter), and 20:5n-3-rich lipid sources such as menhaden oil can be effectively utilized by female white bass to produce quality ova.

Development of pedigree classification using microsatellite and mitochondrial markers for Giant grouper broodstock (Epinephelus lanceolatus) management in Taiwan.

PubMed

Kuo, Hsiao-Che; Hsu, Hao-Hsuan; Chua, Chee Shin; Wang, Ting-Yu; Chen, Young-Mao; Chen, Tzong-Yueh

2014-04-30

Most giant groupers in the market are derived from inbred stock. Inbreeding can cause trait depression, compromising the animals' fitness and disease resistance, obligating farmers to apply increased amounts of drugs. In order to solve this problem, a pedigree classification method is needed. Here, microsatellite and mitochondrial DNA were used as genetic markers to analyze the genetic relationships among giant grouper broodstocks. The 776-bp fragment of high polymorphic mitochondrial D-loop sequence was selected for measuring sibling relatedness. In a sample of 118 giant groupers, 42 haplotypes were categorized, with nucleotide diversity (π) of 0.00773 and haplotype diversity (HD) of 0.983. Furthermore, microsatellites were used for investigation of parentage. Six out of 33 microsatellite loci were selected as markers based on having a high number of alleles and compliance with Hardy-Weinberg equilibrium. Microsatellite profiles based on these loci provide high variability with low combined non-exclusion probability, permitting practical use in aquaculture. The method described here could be used to improve grouper broodstock management and lower the chances of inbreeding. This approach is expected to lead to production of higher quality groupers with higher disease resistance, thereby reducing the need for drug application.

Snake River Sockeye Salmon Captive Broodstock Program; Research Element, 2003 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Willard, Catherine; Plaster, Kurtis; Castillo, Jason

2005-01-01

On November 20, 1991, the National Oceanic Atmospheric Administration listed Snake River sockeye salmon Oncorhynchus nerka as endangered under the Endangered Species Act of 1973. In 1991, the Shoshone-Bannock Tribes (SBT) and Idaho Department of Fish and Game (IDFG) initiated the Snake River Sockeye Salmon Captive Broodstock Program to conserve and rebuild populations in Idaho. Restoration efforts are focused on Redfish, Pettit, and Alturas lakes within the Sawtooth Valley. The first release of hatchery-produced adults occurred in 1993. The first release of juvenile sockeye salmon from the captive broodstock program occurred in 1994. In 1999, the first anadromous adult returnsmore » from the captive broodstock program were recorded when six jacks and one jill were captured at the IDFG Sawtooth Fish Hatchery. In 2003, progeny from the captive broodstock program were released using three strategies: eyed-eggs were planted in Pettit and Alturas lakes in November and December, age-0 presmolts were released to Alturas, Pettit, and Redfish lakes in October, and hatchery-produced adult sockeye salmon were released to Redfish Lake for volitional spawning in September. Oncorhynchus nerka population monitoring was conducted on Redfish, Alturas, and Pettit lakes using a midwater trawl in September 2003. Age-0 through age-4 O. nerka were captured in Redfish Lake, and population abundance was estimated at 81,727 fish. Age-0 through age-3 O. nerka were captured in Alturas Lake, and population abundance was estimated at 46,234 fish. Age-0 through age-3 O. nerka were captured in Pettit Lake, and population abundance was estimated at 11,961 fish. Angler surveys were conducted from May 25 through August 7, 2003 on Redfish Lake to estimate kokanee harvest. On Redfish Lake, we interviewed 179 anglers and estimated that 424 kokanee were harvested. The calculated kokanee catch rate was 0.09 fish/hour. The juvenile out-migrant trap on Redfish Lake Creek was operated from April 15 to May 29, 2003. We estimated that 4,637 wild/natural and 12,226 hatchery-produced sockeye salmon smolts out-migrated from Redfish Lake in 2003. The hatchery-produced component included an estimated 5,352 out-migrants produced from a summer direct-release made to Redfish Lake in 2002 and 6,874 out-migrants produced from a fall direct-release made in 2002. The juvenile out-migrant traps on Alturas Lake Creek and Pettit Lake Creek were operated by the SBT from April 23 to June 5, 2003 and April 25 to June 4, 2003, respectively. The SBT enumerated 28 wild/natural and 13,329 hatchery-produced sockeye salmon smolts that outmigrated from Pettit Lake and estimated 286 wild/natural and 553 hatchery-produced sockeye salmon smolts out-migrated from Alturas Lake in 2003. The hatchery-produced component of sockeye salmon out-migrants originated from presmolt releases made directly to Pettit and Alturas lakes in 2002. Median travel times for passive integrated transponder (PIT) tagged smolts from the Redfish Lake Creek trap site to Lower Granite Dam were estimated for wild/natural smolts and hatchery-produced smolts. Median travel times for smolts originating from the Redfish Lake Creek trap were 10.6 d for wild/natural smolts, 6.2 d for summer direct-released smolts, and 7.1 d for fall direct-released smolts. Median travel times for PIT-tagged smolts from the Pettit Lake Creek trap site to Lower Granite Dam were estimated for hatchery-produced smolts. Median travel times for smolts originating from the Pettit Lake Creek trap were 14.1 d for fall direct released smolts and 13.6 d for fall direct released smolts. Cumulative unique PIT tag interrogations from Sawtooth Valley juvenile out-migrant traps to mainstem Snake and Columbia river dams were utilized to estimate detection rates for out-migrating sockeye salmon smolts. Detection rate comparisons were made between smolts originating from Redfish, Alturas, and Pettit lakes and the various release strategies. Pettit Lake fall direct released smolts recorded the highest detection rate of 37.14%. In 2003, 312 hatchery-produced adult sockeye salmon were released to Redfish Lake for natural spawning. We observed 42 areas of excavation in the lake from spawning events. Suspected redds were approximately 3 m x 3 m in size and were constructed by multiple pairs of adults. We monitored bull trout spawning in Fishhook Creek, a tributary to Redfish Lake, and in Alpine Creek, a tributary to Alturas Lake. This represented the sixth consecutive year that the index reaches have been surveyed on these two streams. Adult counts (40 adults) and redd counts (17 redds) in Fishhook Creek were similar to counts conducted since monitoring began in 1998. Bull trout numbers (27 adults) and the number of redds observed (14 redds) have gradually increased in Alpine Creek compared to counts from initial monitoring.« less

Dietary vitamin A, ascorbic acid and α-tocopherol affect the gonad development and reproductive performance of starry flounder Platichthys stellatus broodstock

NASA Astrophysics Data System (ADS)

Wang, Jiying; Li, Baoshan; Liu, Xudong; Ma, Jingjing; Wang, Shixin; Zhang, Limin

2014-03-01

The present trial was conducted with starry flounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A, ascorbic acid, and α-tocopherol on the gonadal development and reproductive performance. 8 000 IU/kg diet vitamin A (VA group), 500 mg/kg diet ascorbic acid (Vcpp group), or 250 mg/kg diet α-tocopherol (α-TA group) was added into basal diet to create 3 vitamin experimental diets, respectively. Each diet was fed to 450 starry flounder broodstock for 104 days. Samples were collected weekly. The gonadosomatic index (GSI) of 3 vitamin experimental groups first decreased and then increased. Maximum GSI of Vcpp group was higher than that of α-TA group but lower than that of VA group. The spawning periods of 3 vitamin experimental groups lasted 49, 56, and 45 days, respectively. No mature eggs were observed in the control group during the trial. The absolute fecundity (AF) and relative fecundity (RF) of α-TA group was higher than that of Vcpp group but lower than that of VA group. The results suggest that different vitamins play different roles in the fish reproductive process. Vitamin A stimulated the maturation of the ovary, ascorbic acid prolonged the spawning period, and α-tocopherol affected the development of the eggs.

Larval connectivity of pearl oyster through biophysical modelling; evidence of food limitation and broodstock effect

NASA Astrophysics Data System (ADS)

Thomas, Yoann; Dumas, Franck; Andréfouët, Serge

2016-12-01

The black-lip pearl oyster (Pinctada margaritifera) is cultured extensively to produce black pearls, especially in French Polynesia atoll lagoons. This aquaculture relies on spat collection, a process that experiences spatial and temporal variability and needs to be optimized by understanding which factors influence recruitment. Here, we investigate the sensitivity of P. margaritifera larval dispersal to both physical and biological factors in the lagoon of Ahe atoll. Coupling a validated 3D larval dispersal model, a bioenergetics larval growth model following the Dynamic Energy Budget (DEB) theory, and a population dynamics model, the variability of lagoon-scale connectivity patterns and recruitment potential is investigated. The relative contribution of reared and wild broodstock to the lagoon-scale recruitment potential is also investigated. Sensitivity analyses pointed out the major effect of the broodstock population structure as well as the sensitivity to larval mortality rate and inter-individual growth variability to larval supply and to the subsequent settlement potential. The application of the growth model clarifies how trophic conditions determine the larval supply and connectivity patterns. These results provide new cues to understand the dynamics of bottom-dwelling populations in atoll lagoons, their recruitment, and discuss how to take advantage of these findings and numerical models for pearl oyster management.

Development of Pedigree Classification Using Microsatellite and Mitochondrial Markers for Giant Grouper Broodstock (Epinephelus lanceolatus) Management in Taiwan

PubMed Central

Kuo, Hsiao-Che; Hsu, Hao-Hsuan; Chua, Chee Shin; Wang, Ting-Yu; Chen, Young-Mao; Chen, Tzong-Yueh

2014-01-01

Most giant groupers in the market are derived from inbred stock. Inbreeding can cause trait depression, compromising the animals’ fitness and disease resistance, obligating farmers to apply increased amounts of drugs. In order to solve this problem, a pedigree classification method is needed. Here, microsatellite and mitochondrial DNA were used as genetic markers to analyze the genetic relationships among giant grouper broodstocks. The 776-bp fragment of high polymorphic mitochondrial D-loop sequence was selected for measuring sibling relatedness. In a sample of 118 giant groupers, 42 haplotypes were categorized, with nucleotide diversity (π) of 0.00773 and haplotype diversity (HD) of 0.983. Furthermore, microsatellites were used for investigation of parentage. Six out of 33 microsatellite loci were selected as markers based on having a high number of alleles and compliance with Hardy-Weinberg equilibrium. Microsatellite profiles based on these loci provide high variability with low combined non-exclusion probability, permitting practical use in aquaculture. The method described here could be used to improve grouper broodstock management and lower the chances of inbreeding. This approach is expected to lead to production of higher quality groupers with higher disease resistance, thereby reducing the need for drug application. PMID:24796300

Genetic Analysis of Snake River Sockeye Salmon (Oncorhynchus Nerka), 2003 Technical Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Faler, Joyce; Powell, Madison

2003-12-01

A total of 1720 Oncorhynchus nerka tissue samples from 40 populations were characterized using mitochondrial DNA RFLPs (Restriction Fragment Length Polymorphisms). Analysis of anadromous sockeye populations indicated the historical presence of four major maternal lineages. Thirty-five composite mitochondrial haplotypes were observed from the 40 populations of O. nerka sampled throughout the Pacific Northwest. Six of these composite haplotypes ranged in frequency from 7-26% overall and were commonly observed in most populations. The six haplotypes together comprised 90% of the sampled O. nerka. An average of 4.6 composite haplotypes were observed per population. Genetic markers used were satisfactory in separating Redfishmore » Lake anadromous sockeye, residual sockeye and outmigrants from the sympatric kokanee population that spawns in the Fishhook Creek tributary. Outmigrants appear to be primarily composed of progeny from resident residual sockeye, and captively-reared progeny of the captive broodstock program. Thus, residual sockeye may be considered a suitable source of genetic variation to maintain genetic diversity among captive broodstocks of anadromous sockeye. Fishhook Creek kokanee are genetically diverse and during spawning, are temporally and spatially isolated from the residual sockeye population. Eleven composite haplotypes were observed in the kokanee population. The unusually high number of haplotypes is most likely a consequence of periodic stocking of Redfish Lake with kokanee from other sources. Genetic data from Redfish Lake creel samples taken during 1996-1999 putatively indicate the incidental take of a listed resident sockeye.« less

Progress, challenges and perspectives on fish gamete cryopreservation: A mini-review.

PubMed

Asturiano, Juan F; Cabrita, Elsa; Horváth, Ákos

2017-05-01

Protocols for the cryopreservation of fish gametes have been developed for many different fish species, in special, freshwater salmonids and cyprinids. Methods for sperm freezing have progressed during the last decades due to the increasing number of potential applications: aquaculture (genetic improvement programs, broodstock management, helping with species having reproductive problems), biotechnology studies using model fish species (preservation of transgenic or mutant lines), cryobanking of genetic resources from endangered species, etc. This mini-review tries to give an overview of the present situation of this area of research, identifying the main challenges and perspectives, redirecting the reader to more in-depth reviews and papers. Copyright © 2016 Elsevier Inc. All rights reserved.

Identification, Characterization, and Mapping of a Novel SNP Associated with Body Color Transparency in Juvenile Red Sea Bream (Pagrus major).

PubMed

Sawayama, Eitaro; Noguchi, Daiki; Nakayama, Kei; Takagi, Motohiro

2018-03-23

We previously reported a body color deformity in juvenile red sea bream, which shows transparency in the juvenile stage because of delayed chromatophore development compared with normal individuals, and this finding suggested a genetic cause based on parentage assessments. To conduct marker-assisted selection to eliminate broodstock inheriting the causative gene, developing DNA markers associated with the phenotype was needed. We first conducted SNP mining based on AFLP analysis using bulked-DNA from normal and transparent individuals. One SNP was identified from a transparent-specific AFLP fragment, which significantly associated with transparent individuals. Two alleles (A/G) were observed in this locus, and the genotype G/G was dominantly observed in the transparent groups (97.1%) collected from several production lots produced from different broodstock populations. A few normal individuals inherited the G/G genotype (5.0%), but the A/A and A/G genotypes were dominantly observed in the normal groups. The homologs region of the SNP was searched using a medaka genome database, and intron 12 of the Nell2a gene (located on chromosome 6 of the medaka genome) was highly matched. We also mapped the red sea bream Nell2a gene on the previously developed linkage maps, and this gene was mapped on a male linkage group, LG4-M. The newly found SNP was useful in eliminating broodstock possessing the causative gene of the body color transparency observed in juvenile stage of red sea bream.

Evaluation of colorimetric loop-mediated isothermal amplification assay for visual detection of Streptococcus agalactiae and Streptococcus iniae in tilapia.

PubMed

Suebsing, R; Kampeera, J; Tookdee, B; Withyachumnarnkul, B; Turner, W; Kiatpathomchai, W

2013-10-01

Streptococcus agalactiae and Strep. iniae are bacterial pathogens that cause streptococcosis in many fish species. An accelerated colorimetric loop-mediated isothermal amplification (LAMP) assay with pre-addition of calcein was established, and the transmission and detection of Strep. agalactiae and Strep. iniae in tilapia under natural aquatic environment were investigated. A positive reaction was observed by a colour change from orange to green through the naked eyes after completion at 63°C for 30 min with 10 times higher sensitivity than that of nested PCR assays and without cross-amplification with other fish bacterial pathogens. All sample types of Nile and red tilapia (broodstock, fertilized egg, fry) were Strep. agalactiae- and Strep. iniae positive by this new method, implying that they could be vertically transmitted. With its application for screening broodstock and fry before stocking and for monitoring fish health in grow-out ponds, the method would become very useful in fish farming industry. The application of colorimetric LAMP with pre-addition of calcein offers simple, rapid and sensitive technique with applicability for small field laboratories. This technique explored the possible vertical transmission mode of Strep. agalactiae and Strep. iniae under natural aquatic environment. It could be such preliminary data provided for the screening broodstock before breeding and/or the specific-pathogen-free production. © 2013 The Society for Applied Microbiology.

Vibrios Associated with Litopenaeus vannamei Larvae, Postlarvae, Broodstock, and Hatchery Probionts

PubMed Central

Vandenberghe, Johan; Verdonck, Linda; Robles-Arozarena, Rocio; Rivera, Gabriel; Bolland, Annick; Balladares, Marcos; Gomez-Gil, Bruno; Calderon, Jorge; Sorgeloos, Patrick; Swings, Jean

1999-01-01

Several bacteriological surveys were performed from 1994 to 1996 at different Litopenaeus vannamei hatcheries (in Ecuador) and shrimp farms (in Mexico). Samples were taken from routine productions of healthy and diseased L. vannamei larvae, postlarvae, and their culture environment and from healthy and diseased juveniles and broodstock. In Ecuador, the dominant bacterial flora associated with shrimp larvae showing symptoms of zoea 2 syndrome, mysis mold syndrome, and bolitas syndrome has been determined. Strains were characterized by Biolog metabolic fingerprinting and identified by comparison to a database of 850 Vibrio type and reference strains. A selection of strains was further genotypically fine typed by AFLP. Vibrio alginolyticus is predominantly present in all larval stages and is associated with healthy nauplius and zoea stages. AFLP genetic fingerprinting shows high genetic heterogeneity among V. alginolyticus strains, and the results suggest that putative probiotic and pathogenic strains each have specific genotypes. V. alginolyticus was found to be associated with larvae with the zoea 2 syndrome and the mysis mold syndrome, while different Vibrio species (V. alginolyticus and V. harveyi) are associated with the bolitas syndrome. V. harveyi is associated with diseased postlarvae, juveniles, and broodstock. The identities of the strains identified as V. harveyi by the Biolog system could not be unambiguously confirmed by AFLP genomic fingerprinting. Vibrio strain STD3-988 and one unidentified strain (STD3-959) are suspected pathogens of only juvenile and adult stages. V. parahaemolyticus, Photobacterium damselae, and V. mimicus are associated with juvenile and adult stages. PMID:10347048

Managing Polyploidy in Ex Situ Conservation Genetics: The Case of the Critically Endangered Adriatic Sturgeon (Acipenser naccarii)

PubMed Central

Congiu, Leonardo; Pujolar, Jose Martin; Forlani, Anna; Cenadelli, Silvia; Dupanloup, Isabelle; Barbisan, Federica; Galli, Andrea; Fontana, Francesco

2011-01-01

While the current expansion of conservation genetics enables to address more efficiently the management of threatened species, alternative methods for genetic relatedness data analysis in polyploid species are necessary. Within this framework, we present a standardized and simple protocol specifically designed for polyploid species that can facilitate management of genetic diversity, as exemplified by the ex situ conservation program for the tetraploid Adriatic sturgeon Acipenser naccarii. A critically endangered endemic species of the Adriatic Sea tributaries, its persistence is strictly linked to the ex situ conservation of a single captive broodstock currently decimated to about 25 individuals, which represents the last remaining population of Adriatic sturgeon of certain wild origin. The genetic variability of three F1 broodstocks available as future breeders was estimated based on mitochondrial and microsatellite information and compared with the variability of the parental generation. Genetic data showed that the F1 stocks have only retained part of the genetic variation present in the original stock due to the few parent pairs used as founders. This prompts for the urgent improvement of the current F1 stocks by incorporating new founders that better represent the genetic diversity available. Following parental allocation based on band sharing values, we set up a user-friendly tool for selection of candidate breeders according to relatedness between all possible parent-pairs that secures the use of non-related individuals. The approach developed here could also be applied to other endangered tetraploid sturgeon species overexploited for caviar production, particularly in regions lacking proper infrastructure and/or expertise. PMID:21483472

Research on Captive Broodstock Programs for Pacific Salmon, 2000-2001 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry A.; Dittman, Andrew H.; Hardy, Ronald W.

It is not yet possible to define a feeding regimen for captively-reared stocks similar to their natural regimen that enhances the post-release fitness of juveniles and improves the reproductive performance of adults. In the natural environment, seasonal differences in food quality and quantity have profound effects on growth and 'wild' attributes, such as external coloration and fin quality. Formulating the right feeds for conservation fish held for long periods in captivity before release is more complicated than formulating diets for farm fish. Recent research in salmonid nutrition shows it is necessary to consider daily dietary protein intake and protein intakemore » relative to total dietary energy level, rather than simply the levels of total dietary lipid.« less

Fatty Acid Profile of Neutral and Polar Lipid Fraction of Wild Eggs and Hatchlings from Wild and Captive Reared Broodstock of Octopus vulgaris

PubMed Central

Estefanell, Juan; Mesa-Rodríguez, Antonio; Ramírez, Besay; La Barbera, Antonio; Socorro, Juan; Hernandez-Cruz, Carmen María; Izquierdo, María Soledad

2017-01-01

The culture of Octopus vulgaris is constrained by unsolved problems in paralarvae rearing, mainly associated to the unknown nutritional requirements of this species in early stages. In this article we studied the fatty acid profile (total, neutral, and polar lipid fractions) in wild eggs and wild hatchlings, collected in Gran Canaria (SW) (Spain) with artificial dens, in comparison to hatchlings obtained in captivity from broodstock fed on trash fish species. Total lipids were 11.5–13.5% dw, with the polar fraction representing a 70.6–75.5% of total lipid, with lower values in wild hatchling in comparison with captive ones. Docosahexaenoic acid (DHA) was the main component in neutral and polar fatty acid profile in all samples, underlying its importance in this species. Decreasing levels of saturates and arachidonic acid (ARA) from wild eggs to hatchlings, mainly associated to the polar fraction, suggest their use during embryonic development. In hatchlings, increasing levels of oleic acid in the neutral fraction and eicosapentaenoic acid (EPA) in the polar fraction, suggests their importance in hatchlings quality. Wild hatchlings showed in the polar fraction higher oleic acid and ARA, and lower DHA/ARA and EPA/ARA ratios in comparison with captive hatchlings, suggesting a difference in paralarvae nutritional status. These results suggest the importance of n-3 highly unsaturated fatty acids (HUFA), oleic acid, and ARA, presented in the adequate lipid fraction, in the diet of broodstock and paralarvae of O. vulgaris. PMID:28790921

Discovery and partial characterization of a non-LTR retrotransposon that may be associated with abdominal segment deformity disease (ASDD) in the whiteleg shrimp Penaeus (Litopenaeus) vannamei.

PubMed

Sakaew, Waraporn; Pratoomthai, Benjamart; Pongtippatee, Pattira; Flegel, Timothy W; Withyachumnarnkul, Boonsirm

2013-09-30

Abdominal segment deformity disease (ASDD) of cultivated whiteleg shrimp Penaeus (Litopenaeus) vannamei causes economic loss of approximately 10% in affected specimens because of the unsightliness of distorted abdominal muscles. It is associated with the presence of viral-like particles seen by electron microscopy in the ventral nerve cords of affected shrimp. Thus, shotgun cloning was carried out to seek viral-like sequences in affected shrimp. A new retrovirus-like element of 5052 bp (named abdominal segment deformity element or ASDE) was compiled by shotgun cloning and 3' and 5' RACE using RNA and DNA extracted from ventral nerve cords of ASDD shrimp. ASDE contained 7 putative open reading frames (ORF). One ORF (called the PENS sub-domain), had a deduced amino acid (aa) sequence homologous to the GIY-YIG endonuclease domain of penelope-like retrotransposons while two others were homologous to the reverse transcriptase (RT) and RNaseH domains of the pol gene of non-long terminal repeat (non-LTR) retrotransposons (called the NLRS sub-domain). No single amplicon of 5 kb containing both these elements was obtained by PCR or RT-PCR from ASDD shrimp. Subsequent analysis indicated that PENS and NLRS were not contiguous and that NLRS was a host genetic element. In situ hybridization using a dioxygenin-labeled NLRS probe revealed that NLRS gave positive reactions in abdominal-ganglion neurons of ASDD shrimp but not normal shrimp. Preliminary analysis indicated that long-term use of female broodstock after eyestalk ablation in the hatchery increased the intensity of RT-PCR amplicons for NLRS and also the prevalence of ASDD in mysis 3 offspring of the broodstock. The deformities persist upon further cultivation until shrimp harvest but do not increase in prevalence and do not affect growth or survival. Our results suggested that NLRS is a shrimp genetic element associated with ASDD and that immediate preventative measures could include shorter-term use of broodstock after eyestalk ablation and/or discard of broodstock that give strong RT-PCR reactions for NLRS. In the longer term, it is recommended, if possible, that currently used, domesticated shrimp lines be selected for freedom from NLRS. The molecular tools developed in this work will facilitate the management and further study of ASDD.

Discovery and partial characterization of a non-LTR retrotransposon that may be associated with abdominal segment deformity disease (ASDD) in the whiteleg shrimp Penaeus (Litopenaeus) vannamei

PubMed Central

2013-01-01

Background Abdominal segment deformity disease (ASDD) of cultivated whiteleg shrimp Penaeus (Litopenaeus) vannamei causes economic loss of approximately 10% in affected specimens because of the unsightliness of distorted abdominal muscles. It is associated with the presence of viral-like particles seen by electron microscopy in the ventral nerve cords of affected shrimp. Thus, shotgun cloning was carried out to seek viral-like sequences in affected shrimp. Results A new retrovirus-like element of 5052 bp (named abdominal segment deformity element or ASDE) was compiled by shotgun cloning and 3′ and 5′ RACE using RNA and DNA extracted from ventral nerve cords of ASDD shrimp. ASDE contained 7 putative open reading frames (ORF). One ORF (called the PENS sub-domain), had a deduced amino acid (aa) sequence homologous to the GIY-YIG endonuclease domain of penelope-like retrotransposons while two others were homologous to the reverse transcriptase (RT) and RNaseH domains of the pol gene of non-long terminal repeat (non-LTR) retrotransposons (called the NLRS sub-domain). No single amplicon of 5 kb containing both these elements was obtained by PCR or RT-PCR from ASDD shrimp. Subsequent analysis indicated that PENS and NLRS were not contiguous and that NLRS was a host genetic element. In situ hybridization using a dioxygenin-labeled NLRS probe revealed that NLRS gave positive reactions in abdominal-ganglion neurons of ASDD shrimp but not normal shrimp. Preliminary analysis indicated that long-term use of female broodstock after eyestalk ablation in the hatchery increased the intensity of RT-PCR amplicons for NLRS and also the prevalence of ASDD in mysis 3 offspring of the broodstock. The deformities persist upon further cultivation until shrimp harvest but do not increase in prevalence and do not affect growth or survival. Conclusions Our results suggested that NLRS is a shrimp genetic element associated with ASDD and that immediate preventative measures could include shorter-term use of broodstock after eyestalk ablation and/or discard of broodstock that give strong RT-PCR reactions for NLRS. In the longer term, it is recommended, if possible, that currently used, domesticated shrimp lines be selected for freedom from NLRS. The molecular tools developed in this work will facilitate the management and further study of ASDD. PMID:24074037

Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program, 1995-2002 Summary Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoffnagle, Timothy; Carmichael, Richard; Noll, William

2003-12-01

The Grande Ronde Basin once supported large runs of chinook salmon Oncorhynchus tshawytscha and estimated peak escapements in excess of 10,000 occurred as recently as the late 1950's (U.S. Army Corps of Engineers 1975). Natural escapement declines in the Grande Ronde Basin have been severe and parallel those of other Snake River populations. Reduced productivity has primarily been attributed to increased mortality associated with downstream and upstream migration past eight dams and reservoirs in the Snake and Columbia rivers. Reduced spawner numbers, combined with human manipulation of previously important spawning and rearing habitat in the Grande Ronde Basin, have resultedmore » in decreased spawning distribution and population fragmentation of chinook salmon in the Grande Ronde Basin (Figure 1; Table 1). Escapement of spring/summer chinook salmon in the Snake River basin included 1,799 adults in 1995, less than half of the previous record low of 3,913 adults in 1994. Catherine Creek, Grande Ronde River and Lostine River were historically three of the most productive populations in the Grande Ronde Basin (Carmichael and Boyce 1986). However, productivity of these populations has been poor for recent brood years. Escapement (based on total redd counts) in Catherine Creek and Grande Ronde and Lostine rivers dropped to alarmingly low levels in 1994 and 1995. A total of 11, 3 and 16 redds were observed in 1994 in Catherine Creek, upper Grande Ronde River and Lostine River, respectively, and 14, 6 and 11 redds were observed in those same streams in 1995. In contrast, the maximum number of redds observed in the past was 505 in Catherine Creek (1971), 304 in the Grande Ronde River (1968) and 261 in 1956 in the Lostine River (Tranquilli et al 2003). Redd counts for index count areas (a standardized portion of the total stream) have also decreased dramatically for most Grande Ronde Basin streams from 1964-2002, dropping to as low as 37 redds in the 119.5 km in the index survey areas in 1995 from as high as 1,205 redds in the same area in 1969 (Table 1). All streams reached low points (0-6 redds in the index areas) in the 1990's, except those in which no redds were found for several years and surveys were discontinued, such as Spring, Sheep and Indian creeks which had a total of 109 redds in 1969. The Minam and Wenaha rivers are tributaries of the Grande Ronde River located primarily in wilderness areas. Chinook salmon numbers in these two streams (based on redd counts) also decreased dramatically beginning in the early 1970's (Table 1). Since then there have been a few years of increasing numbers of redds but counts have generally been 25-40% of the number seen in the 1960's. No hatchery fish have been released into either of these streams and we monitor them during spawning ground surveys for the presence of hatchery strays. These populations will be used as a type of control for evaluating our supplementation efforts in Catherine Creek, upper Grande Ronde River and Lostine River. In this way, we can attempt to filter out the effects of downstream variables, over which we have no control, when we interpret the results of the captive broodstock program as the F1 and F2 generations spawn and complete their life cycles in the wild. The Grande Ronde Basin Captive Broodstock Program was initiated because these chinook salmon populations had reached critical levels where dramatic and unprecedented efforts were needed to prevent extinction and preserve any future options for use of endemic fish for artificial propagation programs for recovery and mitigation. This program was designed to quickly increase numbers of returning adults, while maintaining the genetic integrity of each endemic population.« less

Effectiveness of an integrated hatchery program: Can genetic-based performance differences between hatchery and wild Chinook salmon be avoided?

USGS Publications Warehouse

Hayes, Michael C.; Reisenbichler, Reginald R.; Rubin, Stephen P.; Drake, Deanne C.; Stenberg, Karl D.; Young, Sewall F.

2013-01-01

Performance of wild (W) and hatchery (H) spring Chinook salmon (Oncorhynchus tshawytscha) was evaluated for a sixth generation hatchery program. Management techniques to minimize genetic divergence from the wild stock included regular use of wild broodstock and volitional releases of juveniles. Performance of HH, WW, and HW (hatchery female spawned with wild male) crosses was compared in hatchery and stream environments. The WW juveniles emigrated from the hatchery at two to three times the rate of HH fish in the fall (HW intermediate) and 35% more HH than WW adults returned (27% more HW than WW adults). Performance in the stream did not differ statistically between HH and WW fish, but outmigrants (38% WW, 30% HW, and 32% HH fish) during the first 39 days of the 16-month sampling period composed 74% of total outmigrants. Differences among hatchery-reared crosses were partially due to additive genetic effects, were consistent with domestication (increased fitness for the hatchery population in the hatchery program), and suggested that selection against fall emigration from the hatchery was a possible mechanism of domestication.

Designation of a neotype for brook trout, Salvelinus fontinalis

USGS Publications Warehouse

Stauffer, Jay R; King, Timothy L.

2015-01-01

The taxonomic status of Salvelinus fontinalis (Mitchill) is problematic. Difficulties in comparison of populations are exacerbated by the lack of type material. Here we designate a neotype from Connetquot River, Long Island, New York. We provide genetic and morphological data for the neotype, conspecifics, and other populations (Swan Creek, Nissequogue Creek) from Long Island, New York. We demonstrate, using molecular markers, that the population from Connetquot River most likely has not been influenced by the major broodstock strains utilized in the Northeast for supplemental and restorative stocking programs. We distinguish the above populations morphologically from lower interior basin populations, represented by fishes from the Pigeon-French Broad drainage, North Carolina and Tennessee. Finally, we position populations from Long Island, New York, within six distinct lineages of S. fontinalis.

Feasibility of dsRNA treatment for post-clearing SPF shrimp stocks of newly discovered viral infections using Laem Singh virus (LSNV) as a model.

PubMed

Saksmerprome, Vanvimon; Charoonnart, Patai; Flegel, Timothy W

2017-05-02

Using post-larvae derived from specific pathogen free (SPF) stocks in penaeid shrimp farming has led to a dramatic increase in production. At the same time, new pathogens of farmed shrimp are continually being discovered. Sometimes these pathogens are carried by shrimp and other crustaceans as persistent infections without gross signs of disease. Thus it is that a 5-generation stock of Penaeus monodon SPF for several pathogens was found, post-stock-development, to be persistently-infected with newly-discovered Laem Singh virus (LSNV). In this situation, the stock developers were faced with destroying their existing stock (developed over a long period at considerable cost) and starting the whole stock development process anew in order to add LSNV to its SPF list. As an alternative, it was hypothesized that injection of complementary dsRNA into viral-infected broodstock prior to mating might inhibit replication of the target virus sufficiently to reduce or eliminate its transmission to their offspring. Subsequent selection of uninfected offspring would allow for post-clearing of LSNV from the existing stock and for conversion of the stock to LSNV-free status. Testing this hypothesis using the LSNV-infected stock described above, we found that transmission was substantially reduced in several treated broodstock compared to much higher transmission in buffer-injected broodstock. Based on these results, the model is proposed for post-clearing of SPF stocks using dsRNA treatment. The model may also be applicable to post-clearing of exceptional, individual performers from grow-out ponds for return to a nucleus breeding center. Copyright © 2017 Elsevier B.V. All rights reserved.

[International trade in living penaeid shrimp between countries in the Pacific and the Atlantic areas and in Europe].

PubMed

Michel, A

1996-06-01

The tremendous development of penaeid shrimp culture across the world over the past twenty years has led to international trade in eggs, larvae and spawners of the best shrimp species for aquaculture. Trade has involved, in particular, the following species: -Penaeus japonicus from Japan to Europe, the Pacific Islands and South America -P. monodon from South-East Asia to almost all tropical countries -P. vannamei and P. stylirostris from countries along the Pacific coast of South and central America to the United States of America, the islands of the South Pacific, countries along the Atlantic coast of the Americas and certain countries in Africa. In the 1980s, research conducted by American and French teams enabled the breeding of stocks of spawners in captivity. This resulted in the establishment of lines of captive broodstock outside natural areas of distribution, and the development of new breeding farms in previously unproductive regions. Trade related to movements of these species is facilitated by the absence of legislation in most countries; even when such legislation does exist, it is not always applied. These practices have led to the rapid development of world shrimp production, but have also contributed to the dissemination of pathogens. In recent years, the occurrence of serious epizootics - occasioning heavy losses world-wide - has led to a more cautious approach involving trade of post-larvae obtained from captive broodstock in which thorough control measures have been implemented for known diseases. Trade in wild shrimp will probably be abandoned progressively, to avoid the risk of introducing new pathogens. The main challenges for research in the near future are the development of diagnostic tools, to enable continuous control of captive broodstock, and the selection of strains which are resistant to the principal pathogens affecting these species.

Grande Ronde Endemic Spring Chinook Salmon Supplementation Program : Facility Operations and Maintenance, 2004 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

McLean, Michael L.; Seeger, Ryan; Hewitt, Laurie

2005-02-01

There were 2 acclimation periods at the Catherine Creek Acclimation Facility (CCAF) in 2004. During the early acclimation period, 92,475 smolts were delivered from Lookingglass Hatchery (LGH) on 8 March. This group was comprised entirely of progeny from the captive broodstock program. The size of the fish at delivery was 23.1 fish/lb. Volitional releases began 15 March 2004 and ended 22 March with an estimated total (based on PIT tag detections of 1,475) of 8,785 fish leaving the raceways. This was 9.5% of the total fish delivered. Fish remaining in the raceways after volitional release were forced out. Hourly detectionsmore » of PIT-tagged fish showed that most of the fish left between 1200 and 2000 hours which was similar to the hourly temperature profile. The size of the fish just before the volitional release was 23.1 and the size of the fish remaining just before the forced release was 23.5 fish/lb. The total mortality for the acclimation period was 62 (0.07 %). The total number of fish released from the acclimation facility during the early period was 92,413. During the second acclimation period 70,977 smolts were delivered from LGH on 24 March. This group was comprised entirely of progeny from the conventional broodstock program. The size of the fish at delivery was 23.4 fish/lb. Volitional releases began 30 March 2004 and ended 12 April with an estimated total (based on PIT tag detections of 3,632) of 49,147 fish leaving the raceways. This was 69.2% of the total fish delivered. Fish remaining in the raceways after volitional release were forced out. Hourly detections of PIT-tagged fish showed that most of the fish left between 1200 and 2000 hours which was similar to the hourly temperature profile. The size of the fish just before the volitional release was 23.4 and the size of the fish remaining just before the forced release was 23.9 fish/lb. The total mortality for the acclimation period was 18 (0.03 %). The total number of fish released from the acclimation facility during the late period was 70,959.« less

Transfering vitamin C from fish to embryos

USDA-ARS?s Scientific Manuscript database

Beneficial effects of ascorbic acid supplementation to broodstock of a select aquaculture species is well documented. At the present levels of feeding, dietary means of vitamin C does not meet the requirements for maturation, reproduction and needs of early life stages of larvae. In addition, thi...

Prevalence of white spot syndrome virus (WSSV) in wild shrimp Penaeus monodon in the Philippines.

PubMed

de la Peña, Leobert D; Lavilla-Pitogo, Celia R; Villar, Corina Belle R; Paner, Milagros G; Sombito, Christopher D; Capulos, Geimbo C

2007-10-15

Prevalence of white spot syndrome virus (WSSV) was determined using polymerase chain reaction (PCR) methodology on DNA extracted from the gills of wild black tiger shrimp Penaeus monodon collected from 7 sampling sites in the Philippines. These 7 sampling sites are the primary sources of spawners and broodstock for hatchery use. During the dry season, WSSV was detected in shrimp from all sites except Bohol, but during the wet season it was not detected in any site except Palawan. None of the WSSV-PCR positive shrimp showed signs of white spots in the cuticle. Prevalence of WSSV showed seasonal variations, i.e. prevalence in dry season (April to May) was higher than in the wet season (August to October). These results suggest that WSSV has already become established in the local marine environment and in wild populations of P. monodon. Thus, broodstock collected during the dry season could serve as the main source of WSSV contamination in shrimp farms due to vertical transmission of the virus in hatcheries.

Preliminary Study on Testicular Germ Cell Transplantation of Endemic Species Oryzias celebensis

NASA Astrophysics Data System (ADS)

Andriani, I.; Agustiani, F.; Hassan, M.; Parenrengi, A.; Inoue, K.

2018-03-01

The research has been conducted to study some technical steps for male germ-plasm from endemic fish species such as some species of Oryzias fish in Indonesia to preserve and propagate through germ cell transplantation technology. For preliminary research, the study was started with germ cell characterization of testes, cryopreservation of TGC and the transplantation of Oryzias celebensis as candidates for surrogate broodstock of Oryzias fish male germ plasm. The data analized included the potential number of TGC as donor, the viability of cryopreserved TGC in two types of cryoprotectans and the survival rate of O.celebensis larvae as recipient after transplantation. The result showed that the average amount of TGC yielded after dissociation was 131000 ± 31349 with 74.2 % viability of TGC each. Cryoprotectan10% DMSO +glucose yielded higher viable of TGC. More than 80 % of O.celebensis larvae survived after transplantation. In conclusion, these preliminary data of O.celebensis as surrogate broodstock candidate will support the application of TGC transplantation technology in Oryzias endemic species.

Salmon Supplementation Studies in Idaho Rivers, 1996-1998 Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reighn, Christopher A.; Lewis, Bert; Taki, Doug

1999-06-01

Information contained in this report summarizes the work that has been done by the Shoshone-Bannock Tribes Fisheries Department under BPA Project No. 89-098-3, Contract Number 92-BI-49450. Relevant data generated by the Shoshone-Bannock Tribe will be collated with other ISS cooperator data collected from the Salmon and Clearwater rivers and tributary streams. A summary of data presented in this report and an initial project-wide level supplementation evaluation will be available in the ISS 5 year report that is currently in progress. The Shoshone-Bannock Tribal Fisheries Department is responsible for monitoring a variety of chinook salmon (Oncorhynchus tshawytscha) production parameters as partmore » of the Idaho Supplementation Studies (BPA Project No. 89-098-3, Contract Number 92-BI-49450). Parameters include parr abundance in tributaries to the upper Salmon River; adult chinook salmon spawner abundance, redd counts, and carcass collection. A rotary screw trap is operated on the East Fork Salmon River and West Fork Yankee Fork Salmon River to enumerate and PIT-tag chinook smolts. These traps are also used to monitor parr movement, and collect individuals for the State and Tribal chinook salmon captive rearing program. The SBT monitors fisheries parameters in the following six tributaries of the Salmon River: Bear Valley Creek, East Fork Salmon River, Herd Creek, South Fork Salmon River, Valley Creek, and West Fork Yankee Fork. Chinook populations in all SBT-ISS monitored streams continue to decline. The South Fork Salmon River and Bear Valley Creek have the strongest remaining populations. Snorkel survey methodology was used to obtain parr population estimates for ISS streams from 1992 to 1997. Confidence intervals for the parr population estimates were large, especially when the populations were low. In 1998, based on ISS cooperator agreement, snorkeling to obtain parr population estimates was ceased due to the large confidence intervals. A rotary screw trap was operated on the West Fork Yankee Fork during the spring, summer, and fall of 1998 to monitor juvenile chinook migration. A screw trap was also operated on the East Fork of the Salmon River during the spring and fall from 1993 to 1997. Supplementation treatments have occurred on the South Fork Salmon River (IDFG), the East Fork Salmon River (EFSR), and the West Fork Yankee Fork of the Salmon River (WFYF). The EFSR received supplementation treatments yearly through 1995. There have been no treatments since 1995, and no significant future treatments from local broodstock are planned due to extremely poor escapement. The WFYF received a single presmolt treatment in 1994. There was an egg and adult release treatment in 1998 from the captive rearing program, not part of the original ISS study. Similarly, no significant future treatments are planned for the West Fork Yankee Fork due to extremely poor escapement. However, small scale experimental captive rearing and broodstock techniques are currently being tested with populations from the EFSR and WFYF. Captive rearing/broodstock techniques could potentially provide feedback for evaluation of supplementation. The other three SBT-ISS streams are control streams and do not receive supplementation treatments.« less

78 FR 79674 - Endangered and Threatened Species; Take of Anadromous Fish

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-31

... and eggs from the Feather River Fish Hatchery (FRFH) and their release into the San Joaquin River or... eggs produced or reared at either the interim facility or the permanent SCARF (from broodstock...,400 CV spring-run Chinook salmon juveniles or 80,000 CV spring-run Chinook salmon eggs originating...

76 FR 63093 - Endangered and Threatened Wildlife and Plants; 12-Month Finding for a Petition To List the...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-11

... several times. Originally, four species of trout were described: Salmo aguabonita from the South Fork Kern... recreational fisheries without any formal genetic consideration to selecting and mating broodstock (group of... in three out of four comparisons, but differences were less consistent when density and biomass were...

Effects of increasing docosahexaenoic acid and arachidonic acid in brood diets of monodactylus sebae on fecundity, egg and larval quality, and egg fatty acid composition

USDA-ARS?s Scientific Manuscript database

Monodactylus sebae is a popular euryhaline ornamental fish species with limited aquaculture production. One of the bottlenecks to their commercial production has been knowledge of broodstock nutritional requirements. Therefore, three brood diets were formulated and fed to M. sebae brood to determine...

Advances in the study on nutrient requirements of grouper (Epinephelus sp.): a review

NASA Astrophysics Data System (ADS)

Luo, Zhi; Liu, Yongjian; Mai, Kangsen; Tian, Lixia

2005-04-01

The paper reviews the recent advances in studying grouper nutrition requirement for the development of cost-effective and environmentally friendly artificial diets. It consists of seven parts: protein and amino acid, lipid and essential fatty acid, carbohydrate, vitamin, mineral, alternative protein source, broodstock and larval nutrition. The review provides some basic information for further investigation of nutrient requirements of groupers.

White bass (Morone chrysops) preferentially retain n-3 PUFA in ova when fed prepared diets with varying FA content

USDA-ARS?s Scientific Manuscript database

We evaluated the fatty acid composition of broodstock white bass ova fed one of the following commercial diets for four and eight weeks prior to sampling: Zeigler BassBrood-45/15, Bio-Oregon BioBrood™-48/20, Cargill AquaFeed™-45/12, Cargill AquaFeed™-48/18, Skretting Extruded Steelhead-45/16, and Sk...

Genetic Diversity of an Imperiled Neotropical Catfish and Recommendations for Its Restoration

PubMed Central

Fonseca, Fernando S.; Domingues, Rodrigo R.; Hallerman, Eric M.; Hilsdorf, Alexandre W. S.

2017-01-01

The long-whiskered catfish Steindachneridion parahybae (Family Pimelodidae) is endemic to the Paraíba do Sul River basin in southeastern Brazil. This species was heavily exploited by artisanal fisheries and faces challenges posed by dams, introduced species, and deterioration of critical habitat. The remaining populations are small and extirpated from some locales, and the species is listed as critically endangered in Brazil. Screening variation at a partial mitochondrial control region sequence (mtCR) and 20 microsatellite loci, we: (i) describe the patterns of genetic diversity along its current distributional range; (ii) test the null hypothesis of panmixia; (iii) investigate the main factors driving its current population structure, and (iv) propose management of broodstock for fostering recovery of wild populations through genetically cognizant restocking. Our microsatellite data for 70 individuals from five collections indicate moderate levels of heterozygosity (HO = 0.45) and low levels of inbreeding (FIS = 0.016). Individual-based cluster analyses showed clear genetic structure, with three clusters of individuals over the collection area with no mis-assigned individuals, suggesting no recent migration among the three clusters. Pairwise DEST values showed moderate and significant genetic differentiation among all populations so identified. The MUR population may have suffered a recent demographic reduction. mtCRs for 70 individuals exhibited 36 haplotypes resulting from 38 polymorphic sites. Overall, mitochondrial haplotype diversity was 0.930 (±0.023) and nucleotide diversity was 0.011 (±0.002). Significant population structure was observed, with ϕST = 0.226. Genetic markers could be used in a hatchery-based restoration program emphasizing breeding of pairs with low kinship values in order to promote retention of genetic diversity and avoid inbreeding. Individual average kinship relationships showed 87.3% advised matings, 11.0% marginal matings, and 1.7% advised against. While these results comprise a contribution toward planning better breeding management and monitoring, parallel actions to be undertaken include surveying healthy riverine habits for reintroduction and continued searching for wild individuals to introduce new variation into the captive broodstock to avoid adaptation to captivity and to minimize inbreeding. PMID:29312433

Genetic Diversity of an Imperiled Neotropical Catfish and Recommendations for Its Restoration.

PubMed

Fonseca, Fernando S; Domingues, Rodrigo R; Hallerman, Eric M; Hilsdorf, Alexandre W S

2017-01-01

The long-whiskered catfish Steindachneridion parahybae (Family Pimelodidae) is endemic to the Paraíba do Sul River basin in southeastern Brazil. This species was heavily exploited by artisanal fisheries and faces challenges posed by dams, introduced species, and deterioration of critical habitat. The remaining populations are small and extirpated from some locales, and the species is listed as critically endangered in Brazil. Screening variation at a partial mitochondrial control region sequence (mtCR) and 20 microsatellite loci, we: (i) describe the patterns of genetic diversity along its current distributional range; (ii) test the null hypothesis of panmixia; (iii) investigate the main factors driving its current population structure, and (iv) propose management of broodstock for fostering recovery of wild populations through genetically cognizant restocking. Our microsatellite data for 70 individuals from five collections indicate moderate levels of heterozygosity ( H O = 0.45) and low levels of inbreeding ( F IS = 0.016). Individual-based cluster analyses showed clear genetic structure, with three clusters of individuals over the collection area with no mis-assigned individuals, suggesting no recent migration among the three clusters. Pairwise D EST values showed moderate and significant genetic differentiation among all populations so identified. The MUR population may have suffered a recent demographic reduction. mtCRs for 70 individuals exhibited 36 haplotypes resulting from 38 polymorphic sites. Overall, mitochondrial haplotype diversity was 0.930 (±0.023) and nucleotide diversity was 0.011 (±0.002). Significant population structure was observed, with ϕ ST = 0.226. Genetic markers could be used in a hatchery-based restoration program emphasizing breeding of pairs with low kinship values in order to promote retention of genetic diversity and avoid inbreeding. Individual average kinship relationships showed 87.3% advised matings, 11.0% marginal matings, and 1.7% advised against. While these results comprise a contribution toward planning better breeding management and monitoring, parallel actions to be undertaken include surveying healthy riverine habits for reintroduction and continued searching for wild individuals to introduce new variation into the captive broodstock to avoid adaptation to captivity and to minimize inbreeding.

"Research to Improve the Efficacy of Captive Broodstock Programs and Advance Hatchery Reform Throughout the Columbia River Basin." [from the Abstract], 2008-2009 Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry A.

This project was developed to conduct research to improve the efficacy of captive broodstock programs and advance hatchery reform throughout the Columbia River Basin. The project has three objectives: (1) maintain adaptive life history characteristics in Chinook salmon, (2) improve imprinting in juvenile sockeye salmon, and (3) match wild phenotypes in Chinook and sockeye salmon reared in hatcheries. A summary of the results are as follows: Objective 1: The ratio of jack to adult male Chinook salmon were varied in experimental breeding populations to test the hypothesis that reproductive success of the two male phenotypes would vary with their relativemore » frequency in the population. Adult Chinook salmon males nearly always obtained primary access to nesting females and were first to enter the nest at the time of spawning. Jack male spawning occurred primarily by establishing satellite positions downstream of the courting pair, and 'sneaking' into the nest at the time of spawning. Male dominance hierarchies were fairly stable and strongly correlated with the order of nest entry at the time of spawning. Observed participation in spawning events and adult-to-fry reproductive success of jack and adult males was consistent with a negative frequency-dependent selection model. Overall, jack males sired an average of 21% of the offspring produced across a range of jack male frequencies. Implications of these and additional findings on Chinook salmon hatchery broodstock management will be presented in the FY 2009 Annual Report. Objective 2: To determine the critical period(s) for imprinting for sockeye salmon, juvenile salmon were exposed to known odorants at key developmental stages. Molecular assessments of imprinting-induced changes in odorant receptor gene expression indicated that regulation of odorant expression is influenced by developmental status and odor exposure history. Expression levels of basic amino acid receptor (BAAR) mRNA in the olfactory epithelium increased dramatically during final maturation in both Stanley Basin and Okanogan River sockeye. These increases appeared to be independent of odor exposure history, rising significantly in both arginine-naive and arginine-exposed fish. However, sockeye exposed to arginine during smolting demonstrated a larger increase in BAAR mRNA than arginine-naive fish. These results are consistent with the hypothesis that odorant receptors sensitive to home stream waters may be upregulated at the time of the homing migration and may afford opportunities to exploit this system to experimentally characterize imprinting success and ultimately identify hatchery practices that will minimize straying of artificially produced salmonids. Additional analysis of Sockeye salmon imprinting and further implications of these findings will be presented in the FY 2009 Annual Report. Objective 3: Photoperiod at emergence and ration after ponding were varied in Yakima River spring Chinook salmon to test the hypothesis that seasonal timing of emergence and growth during early stages of development alter seasonal timing of smoltification and age of male maturation. Fish reared under conditions to advance fry emergence and accelerate growth had the greatest variation in seasonal timing of smolting (fall, spring and summer) and highest rates of early male maturation with most males maturing at age 1 (35-40%). In contrast, fish with delayed emergence and slow growth had the least variation in phenotypes with most fish smolting as yearlings in the spring and no age-1 male maturation. Growth (not emergence timing) altered rates of age-2 male maturation. Results of this study demonstrate that altering fry development, as is often done in hatcheries, can profoundly affect later life history transitions and the range of phenotypes within a spring Chinook salmon population. Additional work in the next funding period will determine if these rearing regimes affected other aspects of smolt quality, which may affect ultimate survival upon ocean entry.« less

Flow-cytometric enrichment of Pacific bluefin tuna type A spermatogonia based on light-scattering properties.

PubMed

Ichida, Kensuke; Kise, Kazuyoshi; Morita, Tetsuro; Yazawa, Ryosuke; Takeuchi, Yutaka; Yoshizaki, Goro

2017-10-01

We previously established surrogate broodstock in which the donor germ cells transplanted into the peritoneal cavities of xenogeneic recipients were capable of developing into functional eggs and sperm in teleost fish. In this transplantation system, only the undifferentiated germ cells such as type A spermatogonia (ASG) or a portion of the ASG population were capable of being incorporated into the genital ridges of the recipients and undergo gametogenesis. Therefore, the use of enriched ASGs can be expected to achieve efficient donor-cell incorporation. Here, we established a method of isolation and enrichment of the ASG of Pacific bluefin tuna using flow cytometry. Whole testicular cell suspensions were fractionated by forward and side scatter properties, following which ASGs were enriched in a fraction in which the forward scatter signal was relatively high and side scatter signal was relatively low. The diameter of sorted cells using the fraction was identical to the size of ASGs observed in histological analysis, and these cells also expressed the vasa gene. In addition, we succeeded in applying this method to several maturation stages of Pacific bluefin tuna. Since this method was based on light-scattering characteristics of ASGs, it can potentially be applied to various teleosts. We expect that this method can contribute to the production of seeds of Pacific bluefin tuna using surrogate broodstock. Copyright © 2017 Elsevier Inc. All rights reserved.

Effects crude protein levels on female Nile tilapia (Oreochromis niloticus) reproductive performance parameters.

PubMed

de Oliveira, Marinez Moraes; Ribeiro, Tainá; Orlando, Tamira Maria; de Oliveira, Dênio Garcia Silva; Drumond, Mariana Martins; de Freitas, Rilke Tadeu Fonseca; Rosa, Priscila Vieira

2014-11-10

The goal of the present work was to study the reproductive performance of Nile Tilapia (Oreochromis niloticus) female broodstock fed diets containing different levels of crude protein (CP). Two hundred and forty Nile tilapia (O. niloticus) were used at an average age of 30 months, with 180 females and 60 males. The broodstock were lodged separately in masonry tanks with continuous water flow. The females were stocked in thirty tanks with dimensions of 8 m(3) in a completely randomized design consisting of five treatments and six replications. The treatments consisted of five diets with different levels of CP (32, 34, 36, 38 and 40%) and with digestible energy per gram of protein of 9.5 kg of feed. The crude protein (CP) levels positively influenced (p<0.05) reproductive parameters (female relative and absolute fecundity, egg diameter, fasting larvae survival capacity), the somatic indexes (gonadosomatic (GSI), hepatosomatic (HIS), viscerosomatic (VSI)), total plasma protein, albumin and triglycerides. There were no significant differences (p>0.05) observed with regard to spawning weight and female weight. The reproductive parameters studied in the present research indicate that diets formulated with 38% CP with digestible energy per gram of CP of 9.5 were the best diets for tilapia females during the reproductive period. Copyright © 2014 Elsevier B.V. All rights reserved.

Association Mapping of Disease Resistance Traits in Rainbow Trout Using Restriction Site Associated DNA Sequencing

PubMed Central

Campbell, Nathan R.; LaPatra, Scott E.; Overturf, Ken; Towner, Richard; Narum, Shawn R.

2014-01-01

Recent advances in genotyping-by-sequencing have enabled genome-wide association studies in nonmodel species including those in aquaculture programs. As with other aquaculture species, rainbow trout and steelhead (Oncorhynchus mykiss) are susceptible to disease and outbreaks can lead to significant losses. Fish culturists have therefore been pursuing strategies to prevent losses to common pathogens such as Flavobacterium psychrophilum (the etiological agent for bacterial cold water disease [CWD]) and infectious hematopoietic necrosis virus (IHNV) by adjusting feed formulations, vaccine development, and selective breeding. However, discovery of genetic markers linked to disease resistance offers the potential to use marker-assisted selection to increase resistance and reduce outbreaks. For this study we sampled juvenile fish from 40 families from 2-yr classes that either survived or died after controlled exposure to either CWD or IHNV. Restriction site−associated DNA sequencing produced 4661 polymorphic single-nucleotide polymorphism loci after strict filtering. Genotypes from individual survivors and mortalities were then used to test for association between disease resistance and genotype at each locus using the program TASSEL. After we accounted for kinship and stratification of the samples, tests revealed 12 single-nucleotide polymorphism markers that were highly associated with resistance to CWD and 19 markers associated with resistance to IHNV. These markers are candidates for further investigation and are expected to be useful for marker assisted selection in future broodstock selection for various aquaculture programs. PMID:25354781

Prevalence, diversity and co-occurrence of the white spot syndrome virus, monodon baculovirus and Penaeus stylirostris densovirus in wild populations of Penaeus monodon in the Philippines.

PubMed

Orosco, Fredmoore L; Lluisma, Arturo O

2017-08-09

The farming of the black tiger shrimp Penaeus monodon in the Philippines relies on wild broodstock. PCR was thus used to determine the prevalence of white spot syndrome virus (WSSV), monodon baculovirus (MBV) and Penaeus stylirostris densovirus (PstDV) in a total of 178 shrimp from 6 geographically disparate locations where broodstock are captured for use in hatcheries. PCR amplicons were also sequenced to identify phylogenetic relationships of the virus haplotypes detected. Shrimp from southeastern Luzon (Camarines Norte) had the highest prevalence of each of the 3 viruses and were frequently co-infected with 2 or more viruses. No viruses were detected in shrimp from northwestern Luzon (Pangasinan). MBV was most prevalent and PstDV strains displayed the most genetic diversity. WSSV was detected at 3 sites, and a VP28 gene sequence examined was invariant and consistent with strains found in many countries, including Thailand, China, Japan, Korea, Indonesia, Iran, Brazil and Mexico. WSSV open reading frame 94 gene sequence analysis identified location-specific repeat types. MBV sequences were dissimilar to haplotypes detected in India. PstDV sequences were diverse and included 2 lineages detected either in Australia or in the United States, Ecuador, Taiwan, China and Vietnam. The PCR data confirmed that WSSV, MBV and PstDV are endemic in P. monodon in the Philippines but that populations at some locations might remain free of infection.

Salmon Supplementation Studies in Idaho Rivers, 1999-2000 Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kohler, Andy; Taki, Doug; Teton, Angelo

2001-11-01

As part of the Idaho Supplementation Studies, fisheries crews from the Shoshone-Bannock Tribes have been snorkeling tributaries of the Salmon River to estimate chinook salmon (Oncorhynchus tshawytscha) parr abundance; conducting surveys of spawning adult chinook salmon to determine the number of redds constructed and collect carcass information; operating a rotary screw trap on the East Fork Salmon River and West Fork Yankee Fork Salmon River to enumerate and PIT-tag emigrating juvenile chinook salmon; and collecting and PIT-tagging juvenile chinook salmon on tributaries of the Salmon River. The Tribes work in the following six tributaries of the Salmon River: Bear Valleymore » Creek, East Fork Salmon River, Herd Creek, South Fork Salmon River, Valley Creek, and West Fork Yankee Fork Salmon River. Snorkeling was used to obtain parr population estimates for ISS streams from 1992 to 1997. However, using the relatively vigorous methods described in the ISS experimental design to estimate summer chinook parr populations, results on a project-wide basis showed extraordinarily large confidence intervals and coefficients of variation. ISS cooperators modified their sampling design over a few years to reduce the variation around parr population estimates without success. Consequently, in 1998 snorkeling to obtain parr population estimates was discontinued and only General Parr Monitoring (GPM) sites are snorkeled. The number of redds observed in SBT-ISS streams has continued to decline as determined by five year cycles. Relatively weak strongholds continue to occur in the South Fork Salmon River and Bear Valley Creek. A rotary screw trap was operated on the West Fork Yankee Fork during the spring and fall of 1999 and the spring of 2000 to monitor juvenile chinook migration. A screw trap was also operated on the East Fork of the Salmon River during the spring and fall from 1993 to 1997 and 1999 (fall only) to 2000. Significant supplementation treatments have occurred in the South Fork Salmon River (IDFG). The East Fork Salmon River received supplementation treatments yearly through 1995. There have been no treatments since 1995, and no significant future treatments from local broodstock are conceivable due to extremely poor escapement. The West Fork Yankee Fork received a single presmolt treatment in 1994. Similarly, no significant future treatments are planned for the WFYF due to extremely poor escapement. However, small scale experimental captive rearing and broodstock techniques are currently being tested with populations from the EFSR and WFYF. Captive rearing/broodstock techniques could potentially provide feedback for evaluation of supplementation. The other three SBT-ISS streams are control streams and do not receive hatchery treatments.« less

New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

PubMed Central

Dubert, Javier; Barja, Juan L.; Romalde, Jesús L.

2017-01-01

Hatcheries constitute nowadays the only viable solution to support the husbandry of bivalve molluscs due to the depletion and/or overexploitation of their natural beds. Hatchery activities include the broodstock conditioning and spawning, rearing larvae and spat, and the production of microalgae to feed all stages of the production cycle. However, outbreaks of disease continue to be the main bottleneck for successful larval and spat production, most of them caused by different representatives of the genus Vibrio. Therefore, attention must be paid on preventive and management measures that allow the control of such undesirable bacterial populations. The present review provides an updated picture of the recently characterized Vibrio species associated with disease of bivalve molluscs during early stages of development, including the controversial taxonomic affiliation of some of them and relevant advances in the knowledge of their virulence determinants. The problematic use of antibiotics, as well as its eco-friendly alternatives are also critically discussed. PMID:28515714

Genetic conservation and paddlefish propagation

USGS Publications Warehouse

Sloss, Brian L.; Klumb, Robert A.; Heist, Edward J.

2009-01-01

The conservation of genetic diversity of our natural resources is overwhelmingly one of the central foci of 21st century management practices. Three recommendations related to the conservation of paddlefish Polyodon spathula genetic diversity are to (1) identify genetic diversity at both nuclear and mitochondrial DNA loci using a suggested list of 20 sampling locations, (2) use genetic diversity estimates to develop genetic management units, and (3) identify broodstock sources to minimize effects of supplemental stocking on the genetic integrity of native paddlefish populations. We review previous genetic work on paddlefish and described key principles and concepts associated with maintaining genetic diversity within and among paddlefish populations and also present a genetic case study of current paddlefish propagation at the U.S. Fish and Wildlife Service Gavins Point National Fish Hatchery. This study confirmed that three potential sources of broodfish were genetically indistinguishable at the loci examined, allowing the management agencies cooperating on this program flexibility in sampling gametes. This study also showed significant bias in the hatchery occurred in terms of male reproductive contribution, which resulted in a shift in the genetic diversity of progeny compared to the broodfish. This shift was shown to result from differential male contributions, partially attributed to the mode of egg fertilization. Genetic insights enable implementation of a paddlefish propagation program within an adaptive management strategy that conserves inherent genetic diversity while achieving demographic goals.

Snake River Sockeye Salmon (Oncorhynchus Nerka) Habitat/Limnologic Research : Annual Report 1992.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Spaulding, Scott

1993-05-01

This report outlines long-term planning and monitoring activities that occurred in 1991 and 1992 in the Stanley Basin Lakes of the upper Salmon River, Idaho for the purpose of sockeye salmon nerka) recovery. Limnological monitoring and experimental sampling protocol, designed to establish a limnological baseline and to evaluate sockeye salmon production capability of the lakes, are presented. Also presented are recommended passage improvements for current fish passage barriers/impediments on migratory routes to the lakes. We initiated O. nerka population evaluations for Redfish and Alturas lakes; this included population estimates of emerging kokanee fry entering each lake in the spring andmore » adult kokanee spawning surveys in tributary streams during the fall. Gill net evaluations of Alturas, Pettit, and Stanley lakes were done in September, 1992 to assess the relative abundance of fish species among the Stanley Basin lakes. Fish population data will be used to predict sockeye salmon production potential within a lake, as well as a baseline to monitor long-term fish community changes as a result of sockeye salmon recovery activities. Also included is a paper that reviews sockeye salmon enhancement activities in British Columbia and Alaska and recommends strategies for the release of age-0 sockeye salmon that will be produced from the current captive broodstock.« less

Genome-wide association study (GWAS) for growth rate and age at sexual maturation in Atlantic salmon (Salmo salar).

PubMed

Gutierrez, Alejandro P; Yáñez, José M; Fukui, Steve; Swift, Bruce; Davidson, William S

2015-01-01

Early sexual maturation is considered a serious drawback for Atlantic salmon aquaculture as it retards growth, increases production times and affects flesh quality. Although both growth and sexual maturation are thought to be complex processes controlled by several genetic and environmental factors, selection for these traits has been continuously accomplished since the beginning of Atlantic salmon selective breeding programs. In this genome-wide association study (GWAS) we used a 6.5K single-nucleotide polymorphism (SNP) array to genotype ∼ 480 individuals from the Cermaq Canada broodstock program and search for SNPs associated with growth and age at sexual maturation. Using a mixed model approach we identified markers showing a significant association with growth, grilsing (early sexual maturation) and late sexual maturation. The most significant associations were found for grilsing, with markers located in Ssa10, Ssa02, Ssa13, Ssa25 and Ssa12, and for late maturation with markers located in Ssa28, Ssa01 and Ssa21. A lower level of association was detected with growth on Ssa13. Candidate genes, which were linked to these genetic markers, were identified and some of them show a direct relationship with developmental processes, especially for those in association with sexual maturation. However, the relatively low power to detect genetic markers associated with growth (days to 5 kg) in this GWAS indicates the need to use a higher density SNP array in order to overcome the low levels of linkage disequilibrium observed in Atlantic salmon before the information can be incorporated into a selective breeding program.

Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey.

PubMed

Betancor, Mónica B; Ortega, Aurelio; de la Gándara, Fernando; Tocher, Douglas R; Mourente, Gabriel

2017-04-01

The present study is the first to evaluate lipid metabolism in first-feeding Atlantic bluefin tuna (ABT; Thunnus thynnus L.) larvae fed different live prey including enriched rotifers Brachionus plicatilis and Acartia sp. copepod nauplii from 2 days after hatch. Understanding the molecular basis of lipid metabolism and regulation in ABT will provide insights to optimize diet formulations for this high-value species new to aquaculture. To this end, we investigated the effect of dietary lipid on whole larvae lipid class and fatty acid compositions and the expression of key genes involved in lipid metabolism in first feeding ABT larvae fed different live prey. Additionally, the expression of lipid metabolism genes in tissues of adult broodstock ABT was evaluated. Growth and survival data indicated that copepods were the best live prey for first feeding ABT and that differences in growth performance and lipid metabolism observed between larvae from different year classes could be a consequence of broodstock nutrition. In addition, expression patterns of lipid metabolic genes observed in ABT larvae in the trials could reflect differences in lipid class and fatty acid compositions of the live prey. The lipid nutritional requirements, including essential fatty acid requirements of larval ABT during the early feeding stages, are unknown, and the present study represents a first step in addressing these highly relevant issues. However, further studies are required to determine nutritional requirements and understand lipid metabolism during development of ABT larvae and to apply the knowledge to the commercial culture of this iconic species.

Prevalence and quantification of pathogenic Vibrio parahaemolyticus during shrimp culture in Thailand.

PubMed

Yingkajorn, Mingkwan; Mitraparp-Arthorn, Pimonsri; Nuanualsuwan, Suphachai; Poomwised, Ratanaruji; Kongchuay, Noochanat; Khamhaeng, Natchaya; Vuddhakul, Varaporn

2014-12-02

Vibrio parahaemolyticus is a major cause of seafood-borne gastroenteritis. The human pathogenic strains possess tdh or trh or both genes. In Thai shrimp farming, the level of pathogenic V. parahaemolyticus contamination has not been completely characterized, although it has been identified as a risk for people who consume undercooked shrimp. In this study, the prevalence and concentration of V. parahaemolyticus (total Vp) and pathogenic V. parahaemolyticus (tdh+ Vp and trh+ Vp) were investigated during shrimp culture cycles using the most probable number (MPN) method and were confirmed by PCR and the loop-mediated isothermal amplification (LAMP) techniques. The prevalence and concentration of total Vp were high in broodstock and egg samples at the start of the hatchery cycle, but the organism decreased in the subsequent larval and postlarval stages. In contrast, total Vp was low at the beginning of the pond cycle and dramatically increased during the later stages of culture. Broodstock and fresh feed were important sources of V. parahaemolyticus. Numbers of tdh+ Vp and trh+ Vp detected by the LAMP technique were much greater than those detected by the PCR technique, especially in the late stages of the pond cycle. A direct correlation between total Vp and pathogenic Vp was demonstrated only during the harvest stage. This study will be useful as a guideline to establish levels of V. parahaemolyticus presence which can be considered as safe during shrimp culture. In addition, it could be used to identify the source of V. parahaemolyticus, which has recently been reported to be one of the etiologic agents of acute hepatopancreatic necrosis disease.

Grande Ronde Endemic Spring Chinook Project - ODFW, 2008 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Patterson, Scott

2009-04-10

Core activities of the Grande Ronde Endemic Spring Chinook Supplementation Program (GRESCSP) are funded through the authority of the Lower Snake River Fish and Wildlife Compensation Plan (LSRCP). The LSRCP program was approved by the Water Resources Development Act of 1976, PL 94-587, Section 102, 94th Congress substantially in accordance with the Special Report, LSRCP, June 1975 on file with the Chief of Engineers. The LSRCP was prepared and submitted in compliance with the Fish and Wildlife Coordination Act of 1958, PL 85-624, 85th Congress, August 12, 1958 to mitigate for the losses of fish and wildlife caused by themore » construction of dams on lower Snake River. The GRESCSP is an artificial propagation program that was initiated by Bonneville Power Administrations Fish and Wildlife program in the mid 1990's. The intent of this program was to change the mitigation aspect of the LSRCP program (harvest mitigation) to an integrated supplementation program; inasmuch as, hatchery produced fish could be experimentally used as a recovery tool and fish surplus to mitigation would be available for in-place and in-kind harvest. Fish production is still authorized by the LSRCP with the original mitigation return goal of 5,860 adult spring Chinook to the project area. The GRESCSP was developed with two primary components: (1) conventional broodstock (projects 199800702; 199800703; 199800704) and (2) captive brood (projects 199801001; 199801006). The GRESCSP relies on cooperative M&E efforts from the LSRCP including setting aside the Wenaha and Minam tributaries as natural production reserves components used for reference streams. The GRESCSP, coordinated with federal and tribal partners, identifies production levels for both propagation components and weir management strategies for each of the three supplemented tributary areas within the Grande Ronde Sub-basin. The three supplemented areas are Catherine Creek, Lostine River, and upper Grande Ronde River. Lookingglass Creek, an extirpated area, will be stocked (smolts and adults) with Catherine Creek origin salmon to initiate natural production in unseeded habitat, and to initiate future harvest opportunities. The current production levels have been incorporated into the U.S. v. Oregon Interim Management Agreement. The purpose of this contract is to integrate Bonneville Power Administration (BPA) efforts with the Lower Snake River Compensation Plan (LSRCP) program utilizing Lookingglass Hatchery as the primary rearing facility. BPA constructed an adult holding and spawning structure on the hatchery grounds; however, maintenance of this infrastructure was discontinued due to funding limitation and transferred to the LSRCP program in 2007. These integrated efforts focus on holding and spawning adults, rearing juveniles, fish health, and monitoring natural production (Redd counts) for Catherine Creek, Lostine River, and Upper Grande Ronde stocks.« less

Cryobanking of aquatic species.

PubMed

Martínez-Páramo, Sonia; Horváth, Ákos; Labbé, Catherine; Zhang, Tiantian; Robles, Vanesa; Herráez, Paz; Suquet, Marc; Adams, Serean; Viveiros, Ana; Tiersch, Terrence R; Cabrita, Elsa

2017-04-01

This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

Brood Year 2004: Johnson Creek Chinook Salmon Supplementation Report, June 2004 through March 2006.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gebhards, John S.; Hill, Robert; Daniel, Mitch

The Nez Perce Tribe, through funding provided by the Bonneville Power Administration, has implemented a small scale chinook salmon supplementation program on Johnson Creek, a tributary in the South Fork of the Salmon River, Idaho. The Johnson Creek Artificial Propagation Enhancement project was established to enhance the number of threatened Snake River spring/summer chinook salmon (Oncorhynchus tshawytscha) returning to Johnson Creek to spawn through artificial propagation. This was the sixth season of adult chinook broodstock collection in Johnson Creek following collections in 1998, 2000, 2001, 2002, and 2003. Weir installation was completed on June 21, 2004 with the first chinookmore » captured on June 22, 2004 and the last fish captured on September 6, 2004. The weir was removed on September 18, 2004. A total of 338 adult chinook, including jacks, were captured during the season. Of these, 211 were of natural origin, 111 were hatchery origin Johnson Creek supplementation fish, and 16 were adipose fin clipped fish from other hatchery operations and therefore strays into Johnson Creek. Over the course of the run, 57 natural origin Johnson Creek adult chinook were retained for broodstock, transported to the South Fork Salmon River adult holding and spawning facility and held until spawned. The remaining natural origin Johnson Creek fish along with all the Johnson Creek supplementation fish were released upstream of the weir to spawn naturally. Twenty-seven Johnson Creek females were artificially spawned with 25 Johnson Creek males. Four females were diagnosed with high bacterial kidney disease levels resulting in their eggs being culled. The 27 females produced 116,598 green eggs, 16,531 green eggs were culled, with an average eye-up rate of 90.6% resulting in 90,647 eyed eggs. Juvenile fish were reared indoors at the McCall Fish Hatchery until November 2005 and then transferred to the outdoor rearing facilities during the Visual Implant Elastomer tagging operation. These fish continued rearing in the outdoor collection basin until release in March 2006. All of these fish were marked with Coded Wire Tags and Visual Implant Elastomer tags. In addition 12,056 of the smolts released were also tagged with Passive Integrated Transponder tags. Hand counts provided by marking crews were used to amend the number of juvenile salmon released from the original egg count. A total of 90,450 smolts were released directly into Johnson Creek on March 13 through 15, 2006.« less

Identification of genes involved in reproduction and lipid pathway metabolism in wild and domesticated shrimps.

PubMed

Rotllant, Guiomar; Wade, Nicholas M; Arnold, Stuart J; Coman, Gregory J; Preston, Nigel P; Glencross, Brett D

2015-08-01

The aims of this study were to identify genes involved in reproduction and lipid pathway metabolism in Penaeus monodon and correlate their expression with reproductive performance. Samples of the hepatopancreas and ovaries were obtained from a previous study of the reproductive performance of wild and domesticated P. monodon broodstock. Total mRNA from the domesticated broodstock was used to create two next generation sequencing cDNA libraries enabling the identification of 11 orthologs of key genes in reproductive and nutritional metabolic pathways in P. monodon. These were identified from the library of de novo assembled contigs, including the description of 6 newly identified genes. Quantitative RT-PCR of these genes in the hepatopancreas prior to spawning showed that the domesticated mature females significantly showed higher expression of the Pm Elovl4, Pm COX and Pm SUMO genes. The ovaries of domesticated females had a significantly decreased expression of the Pm Elovl4 genes. In the ovaries of newly spawned females, a significant correlation was observed between hepatosomatic index and the expression of Pm FABP and also between total lipid content and the expression of Pm CYP4. Although not significant, the highest levels of correlation were found between relative fecundity and Pm CRP and Pm CYP4 expression, and between hatching rate and Pm Nvd and Pm RXR expression. This study reports the discovery of genes involved in lipid synthesis, steroid biosynthesis and reproduction in P. monodon. These results indicate that genes encoding enzymes involved in lipid metabolism pathways might be potential biomarkers to assess reproductive performance. Copyright © 2015 Elsevier B.V. All rights reserved.

Tangential flow ultrafiltration for detection of white spot syndrome virus (WSSV) in shrimp pond water.

PubMed

Alavandi, S V; Ananda Bharathi, R; Satheesh Kumar, S; Dineshkumar, N; Saravanakumar, C; Joseph Sahaya Rajan, J

2015-06-15

Water represents the most important component in the white spot syndrome virus (WSSV) transmission pathway in aquaculture, yet there is very little information. Detection of viruses in water is a challenge, since their counts will often be too low to be detected by available methods such as polymerase chain reaction (PCR). In order to overcome this difficulty, viruses in water have to be concentrated from large volumes of water prior to detection. In this study, a total of 19 water samples from aquaculture ecosystem comprising 3 creeks, 10 shrimp culture ponds, 3 shrimp broodstock tanks and 2 larval rearing tanks of shrimp hatcheries and a sample from a hatchery effluent treatment tank were subjected to concentration of viruses by ultrafiltration (UF) using tangential flow filtration (TFF). Twenty to 100l of water from these sources was concentrated to a final volume of 100mL (200-1000 fold). The efficiency of recovery of WSSV by TFF ranged from 7.5 to 89.61%. WSSV could be successfully detected by PCR in the viral concentrates obtained from water samples of three shrimp culture ponds, one each of the shrimp broodstock tank, larval rearing tank, and the shrimp hatchery effluent treatment tank with WSSV copy numbers ranging from 6 to 157mL(-1) by quantitative real time PCR. The ultrafiltration virus concentration technique enables efficient detection of shrimp viral pathogens in water from aquaculture facilities. It could be used as an important tool to understand the efficacy of biosecurity protocols adopted in the aquaculture facility and to carry out epidemiological investigations of aquatic viral pathogens. Copyright © 2015 Elsevier B.V. All rights reserved.

Larval Dispersal Modeling of Pearl Oyster Pinctada margaritifera following Realistic Environmental and Biological Forcing in Ahe Atoll Lagoon

PubMed Central

Thomas, Yoann; Dumas, Franck; Andréfouët, Serge

2014-01-01

Studying the larval dispersal of bottom-dwelling species is necessary to understand their population dynamics and optimize their management. The black-lip pearl oyster (Pinctada margaritifera) is cultured extensively to produce black pearls, especially in French Polynesia's atoll lagoons. This aquaculture relies on spat collection, a process that can be optimized by understanding which factors influence larval dispersal. Here, we investigate the sensitivity of P. margaritifera larval dispersal kernel to both physical and biological factors in the lagoon of Ahe atoll. Specifically, using a validated 3D larval dispersal model, the variability of lagoon-scale connectivity is investigated against wind forcing, depth and location of larval release, destination location, vertical swimming behavior and pelagic larval duration (PLD) factors. The potential connectivity was spatially weighted according to both the natural and cultivated broodstock densities to provide a realistic view of connectivity. We found that the mean pattern of potential connectivity was driven by the southwest and northeast main barotropic circulation structures, with high retention levels in both. Destination locations, spawning sites and PLD were the main drivers of potential connectivity, explaining respectively 26%, 59% and 5% of the variance. Differences between potential and realistic connectivity showed the significant contribution of the pearl oyster broodstock location to its own dynamics. Realistic connectivity showed larger larval supply in the western destination locations, which are preferentially used by farmers for spat collection. In addition, larval supply in the same sectors was enhanced during summer wind conditions. These results provide new cues to understanding the dynamics of bottom-dwelling populations in atoll lagoons, and show how to take advantage of numerical models for pearl oyster management. PMID:24740288

Low impact of infectious hypodermal and hematopoietic necrosis virus (IHHNV) on growth and reproductive performance of Penaeus monodon.

PubMed

Withyachumnarnkul, Boonsirm; Chayaburakul, Kanokporn; Lao-Aroon, Supak; Plodpai, Pornthep; Sritunyalucksana, Kallaya; Nash, Gary

2006-04-06

No controlled studies on the effect of infectous hypodermal and necrosis virus (IHHNV) on Penaeus monodon have been previously reported. Here we describe domesticated P. monodon that became positive for IHHNV and other viruses at variable levels of prevalence during cultivation in 16 open-air, earthen ponds. These were stocked with domesticated postlarvae (PL) that tested negative for 7 shrimp viruses including IHHNV at 6% prevalence in 3 checks using polymerase chain reaction (PCR) methods. These PL were derived from domesticated female broodstock that individually tested negative for the same viruses. At 4 mo of culture, the shrimp in some ponds without obvious mortality tested positive by PCR methods for IHHNV and 3 other viruses at variable levels of maximum estimated prevalence (MEP). Stained tissue sections showed no lesions typical of IHHNV, but in situ hybridization tests with an IHHNV-specific DNA probe were positive. There was no significant difference in mean body weight (i.e. ca. 25 g) between shrimp groups positive or negative for IHHNV. Similar results were obtained with IHHNV negative and positive adults at 1 yr. Adults that individually tested negative for all 7 viruses and some that tested lightly positive for IHHNV were bred for the next generation. There were no significant differences in the number of eggs (> 600 000) and nauplii (ca. 300,000) produced by females negative and positive for IHHNV. From these females, 11/49 (22%) IHHNV PCR-positive PL batches were obtained from PCR-negative spawners, while 8/11 (73%) were obtained from IHHNV PCR-positive spawners. The results suggested that IHHNV infection can be transmitted vertically but does not seriously retard growth of P. monodon or affect fecundity of lightly infected broodstock.

Induced reproduction and early development histology of Oscar Astronotus ocellatus (Agassiz, 1831).

PubMed

Paes, Maria do Carmo Faria; Makino, Lilian Cristina; Vasquez, Leonardo Avendaño; Fernandes, João Batista Kochenborger; Valentin, Fernanda Nogueira; Nakaghi, Laura Satiko Okada

2015-04-01

Oscar (Astronotus ocellatus) is an important fish from the Amazon Basin that has great potential for fish farming, human consumption, sport fishing and fish keeping. This study aimed to evaluate the effect of two hormonal treatments on the induction of artificial reproduction in broodstock and to describe the histological development of embryos and larvae. Broodstocks were selected and induced using two different hormones: (i) extract of carp pituitary (ECP); and (ii) synthetic human chorionic gonadotropin (hCG). Spawnings were transferred to hatcheries, collected at pre-established times, processed and analysed by histology. Astronotus ocellatus did not respond well to induced reproduction. From 16 couples of breeding fish, only five out of the eight females released oocytes after the hormonal action time, three with hCG and two with ECP; just one male responded positively to hCG. Oscar eggs were oval, and semi-adhesive, the yolk contained granules, and egg diameter was approximately 1.65 ± 0.057 to 1.98 ± 0.038 mm. Development from the initial collection (IC) point until the total absorption of the yolk lasted 315 h, at an average temperature of 27.45 ± 2.13°C. Several events marked embryonic and larval development, including the formation of the optic cup, forebrain, otic vesicle and cephalic divisions. The newly hatched larvae had non-pigmented eyes, and a closed mouth and anus, as well as the presence of adhesive glands on the head. Larval development was characterized by formation of the heart, liver, gaseous bladder, gills, pronephros, brain, fins and also the digestive tract. These results provide important information for the rearing and reproduction of A. ocellatus.

Development and experimental validation of a 20K Atlantic cod (Gadus morhua) oligonucleotide microarray based on a collection of over 150,000 ESTs.

PubMed

Booman, Marije; Borza, Tudor; Feng, Charles Y; Hori, Tiago S; Higgins, Brent; Culf, Adrian; Léger, Daniel; Chute, Ian C; Belkaid, Anissa; Rise, Marlies; Gamperl, A Kurt; Hubert, Sophie; Kimball, Jennifer; Ouellette, Rodney J; Johnson, Stewart C; Bowman, Sharen; Rise, Matthew L

2011-08-01

The collapse of Atlantic cod (Gadus morhua) wild populations strongly impacted the Atlantic cod fishery and led to the development of cod aquaculture. In order to improve aquaculture and broodstock quality, we need to gain knowledge of genes and pathways involved in Atlantic cod responses to pathogens and other stressors. The Atlantic Cod Genomics and Broodstock Development Project has generated over 150,000 expressed sequence tags from 42 cDNA libraries representing various tissues, developmental stages, and stimuli. We used this resource to develop an Atlantic cod oligonucleotide microarray containing 20,000 unique probes. Selection of sequences from the full range of cDNA libraries enables application of the microarray for a broad spectrum of Atlantic cod functional genomics studies. We included sequences that were highly abundant in suppression subtractive hybridization (SSH) libraries, which were enriched for transcripts responsive to pathogens or other stressors. These sequences represent genes that potentially play an important role in stress and/or immune responses, making the microarray particularly useful for studies of Atlantic cod gene expression responses to immune stimuli and other stressors. To demonstrate its value, we used the microarray to analyze the Atlantic cod spleen response to stimulation with formalin-killed, atypical Aeromonas salmonicida, resulting in a gene expression profile that indicates a strong innate immune response. These results were further validated by quantitative PCR analysis and comparison to results from previous analysis of an SSH library. This study shows that the Atlantic cod 20K oligonucleotide microarray is a valuable new tool for Atlantic cod functional genomics research.

The effect of night illumination, red and infrared light, on locomotor activity, behaviour and melatonin of Senegalese sole (Solea senegalensis) broodstock.

PubMed

Carazo, I; Norambuena, F; Oliveira, C; Sánchez-Vázquez, F J; Duncan, N J

2013-06-13

The present study aimed to determine a non-invasive nocturnal lighting system for the behavioural observation of a highly light sensitive species, Senegalese sole (Solea senegalensis). Locomotor activity, four types of behaviour and plasma melatonin were analysed in groups of 12 adult Senegalese sole (Solea senegalensis) reared in captivity and held under four night illumination treatments: total darkness (control), high 50lux intensity red light (group RH), low 5lux intensity red light (group RL) and infrared light (group IR). All groups experienced the same conditions during the day (lights on from 07:00 to 19:00) with white lighting of 125lux. Clarity of video images taken at night for the observation of fish behaviour were ranked as follows: group RH>RL>IR>control. All treatments presented a daily rhythm in locomotor activity with high activity from 14:00 to 18:00 and low activity from 21:00 to 12:00. The sole exposed to the high intensity red light at night appeared to be disturbed as during the low nocturnal locomotor activity period group RH presented higher activity and significantly higher nocturnal behaviour related to escape or fear than was observed in the other groups. The groups control, RL and IR exhibited similar levels of nocturnal locomotor activity and nocturnal behaviour related to escape or fear. Plasma melatonin, at mid-dark was not significantly different between the control and groups RL and IR, while melatonin was significantly lower in group RH compared to the control. The authors recommended low intensity red night illumination for the non-invasive study of nocturnal behaviour of Senegalese sole adults. Copyright © 2013 Elsevier Inc. All rights reserved.

Uptake and selective partitioning of dietary lipids to ovarian and muscle tissue of maturing female coho salmon, Oncorhynchus kisutch, during secondary oocyte growth.

PubMed

Johnson, Ronald B; Kroeger, Eric L; Reichert, William L; Carter, Cameron S; Rust, Michael B

2017-06-01

Female coho salmon, Oncorhynchus kisutch, were fed one of two experimental feeds containing lipids with markedly different stable 13 C isotope signatures during the late cortical alveolus, lipid droplet, and vitellogenesis stages of secondary oocyte growth. Ovarian and muscle lipids fatty acid concentrations were significantly affected by treatment during all three stages of development. Stable 13 C isotope analyses confirmed that dietary lipids were incorporated into both ovarian and muscle lipids during all three stages and revealed that ovarian lipids were more affected than muscle lipids during vitellogenesis. Arachidonic acid (ARA) was incorporated into ovarian lipids at the highest rate of all fatty acids examined with the greatest uptake observed during the cortical alveolus and lipid droplet stages of development. Docosahexaenoic acid (DHA) was incorporated into ovarian lipids at the next highest rate with the greatest uptake observed during the lipid droplet stage of development. The presence of an ovary specific, fatty acid transfer mechanism is proposed. Results from this study demonstrate the ability to greatly alter the fatty acid composition of ovarian lipids through a dietary change during secondary oocyte growth and may be of great interest to producers of farmed salmon and salmon broodstock programs. Published by Elsevier Inc.

Cryobanking of aquatic species

PubMed Central

Martínez-Páramo, Sonia; Horváth, Ákos; Labbé, Catherine; Zhang, Tiantian; Robles, Vanesa; Herráez, Paz; Suquet, Marc; Adams, Serean; Viveiros, Ana; Tiersch, Terrence R.; Cabrita, Elsa

2017-01-01

This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production. PMID:29276317

Ameliorative effect of vitamin E on hepatic oxidative stress and hypoimmunity induced by high-fat diet in turbot (Scophthalmus maximus).

PubMed

Jia, Yudong; Jing, Qiqi; Niu, Huaxin; Huang, Bin

2017-08-01

This study was conducted to examine the effects of vitamin E on growth performance, oxidative stress and non-specific immunity of turbot (Scophthalmus maximus) fed with high-fat diet. Results showed that high-fat diet significantly increased hepatosomatic index, viscerosomatic index, hepatic malondialdehyde level and decreased catalase and superoxide dismutase activities, whereas final weight, specific growth rate and survival rate remained unchanged. Meanwhile, nitro blue tetrazolium positive leucocytes of head kidney, respiratory burst activity in head-kidney macrophage, phagocytic index and serum lysozyme activity were significantly reduced after feeding with high-fat diet. Furthermore, fish fed with high-fat diet promoted higher expression of heat shock protein (hsp70, hsp90), and inhibited expression of complement component 3 (c3) in the liver and tumor necrosis factor-α (tnf-α), interleukine 1β (il-1β), toll like receptor 22 (tlr-22) in the spleen and head-kidney, respectively. However, simultaneous supplementation with 480 mg kg -1 vitamin E protected turbot against high-fat diet-induced hepatic oxidative stress, hypoimmunity through attenuating lipid peroxidation, renewing antioxidant enzymes activities and nonspecific immune responses, and modulating the expression of stress protein (hsp70, hsp90) and immune-related genes (c3, tnf-α, il-1β, tlr-22). In conclusion, the obtained results indicate the vitamin E as a wildly used functional feed additive contributes potentially to alleviate high-fat diet-induced hepatic oxidative stress and hypoimmunity, maintain the health, and improve the broodstock management for turbot. Copyright © 2017 Elsevier Ltd. All rights reserved.

Isolation and characterization of microsatellite loci for alligator gar (Atractosteus spatula) and their variability in two other species (Lepisosteus oculatus and L. osseus) of Lepisosteidae

USGS Publications Warehouse

Moyer, G.R.; Sloss, Brian L.; Kreiser, B.R.; Feldheim, K.A.

2009-01-01

We report on the isolation of 17 polymorphic microsatellite loci from alligator gar (Atractosteus spatula), a large-bodied species that has experienced population declines across much of its range. These loci possessed 2-19 alleles and observed heterozygosities of 0-0.974. All loci conformed to Hardy-Weinberg equilibrium expectations, and none exhibited linkage disequilibrium. Nine and eight of these loci were found to be polymorphic in the related species Lepisosteus oculatus and L. osseus, respectively. These microsatellite loci should prove useful in conservation efforts of A. spatula through the study of population structure and hatchery broodstock management. ?? 2009 Blackwell Publishing Ltd.

Isolation and characterization of microsatellite loci for mountain mullet (Agonostomus monticola).

PubMed

Feldheim, Kevin A; Sanchez, Patrick J; Matamoros, Wilfredo A; Schaefer, Jacob F; Kreiser, Brian R

2009-11-01

We report on the isolation of 15 polymorphic microsatellite loci from mountain mullet (Agonostomus monticola). In the two populations sampled, loci exhibited two to 21 alleles and observed heterozygosity values ranged from 0.222 to 1.000. All loci conformed to Hardy-Weinberg equilibrium expectations, and none exhibited linkage disequilibrium. Although A. monticola is an important subsistence fishery in parts of its range, little is known about its ecology and many populations appear to be experiencing declines. These microsatellite loci should prove useful in the study of population structure of A. monticola and aid in other potential conservation efforts such as the management of hatchery broodstock. © 2009 Blackwell Publishing Ltd.

Sherman Creek Hatchery; Washington Department of Fish and Wildlife Fish Program, 2001 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Combs, Mitch

2002-01-01

Sherman Creek Hatchery's primary objective is the restoration and enhancement of the recreational and subsistence fishery in Lake Roosevelt and Banks Lake. The Sherman Creek Hatchery (SCH) was designed to rear 1.7 million kokanee fry for acclimation and imprinting during the spring and early summer. Additionally, it was designed to trap all available returning adult kokanee during the fall for broodstock operations and evaluations. Since the start of this program, the operations on Lake Roosevelt have been modified to better achieve program goals. The Washington Department of Fish and Wildlife, Spokane Tribe of Indians and the Colville Confederated Tribe formmore » the interagency Lake Roosevelt Hatcheries Coordination Team (LRHCT) which sets goals and objectives for both Sherman Creek and the Spokane Tribal Hatchery and serves to coordinate enhancement efforts on Lake Roosevelt and Banks Lake. The primary changes have been to replace the kokanee fingerling program with a yearling (post smolt) program of up to 1,000,000 fish. To construct and operate twenty net pens to handle the increased production. The second significant change was to rear up to 300,000 rainbow trout fingerling at SCH from July through October, for stocking into the volunteer net pens. This enables the Spokane Tribal Hatchery (STH) to rear additional kokanee to further the enhancement efforts on Lake Roosevelt. Current objectives include increased use of native/indigenous stocks where available for propagation into Upper Columbia River Basin Waters. Monitoring and evaluation is preformed by the Lake Roosevelt Fisheries Monitoring Program. From 1988 to 1998, the principle sport fishery on Lake Roosevelt has shifted from walleye to include rainbow trout and kokanee salmon (Underwood et al. 1997, Tilson and Scholz 1997). The angler use, harvest rates for rainbow and kokanee and the economic value of the fishery has increased substantially during this 10-year period. The most recent information from the monitoring program also suggests that the hatchery and net pen rearing programs have been beneficial to enhancing the Lake Roosevelt fishery while not negatively impacting wild and native stocks within the lake. The 2001 fishing season has been especially successful with great fishing for both rainbow and kokanee throughout Lake Roosevelt. The results of the Two Rivers Fishing Derby identified 100 percent of the rainbow and 47 percent of the kokanee caught were of hatchery origin.« less

White Bass (Morone chrysops) Preferentially Retain n-3 PUFA in Ova When Fed Prepared Diets with Varying FA Content.

PubMed

Fuller, S Adam; Rawles, Steven D; McEntire, Matthew E; Bader, Troy J; Riche, Marty; Beck, Benjamin H; Webster, Carl D

2017-10-01

We evaluated the fatty acid (FA) composition of broodstock white bass ova fed one of six commercial diets with increasing polyunsaturated FA content (n-6/n-3 ratio; 0.36, 0.39, 0.46, 0.83, 1.07, 1.12) eight weeks prior to sampling. Fatty acid profiles of ova from brooders fed each of the six diets were significantly altered according to canonical discriminant analysis. Ova FA profiles resulting from the 0.39 diet separated those from the 0.36 diet based on lower 18:2n-6 (LNA) and higher 20:1n-9 concentrations from the 0.36 diet. Ova profiles were further separated based on lower concentrations of 22:5n-3 (DPA) from the 0.46 diet, lower concentrations of 20:5n-3 (EPA) in the 1.12 and 0.83 diets, and lower concentrations of 22:6n-3 (DHA) in all other diets relative to the 0.46 diet. Changes in ova FA profile at four and eight weeks were consistent with dietary intake with an approximate 2% increase in any given FA class with increasing time on individual diet. There was no correlation between dietary ARA concentrations (0.7-1.1 mol%), or dietary EPA/ARA ratios (7-15), and the concentrations (1.4-1.7 mol%) or ratios (3.3-4.4) found in the ova by diet. Our results suggest that white bass females have the ability to preferentially incorporate n-3 PUFA, particularly DHA, suggesting mobilization of this FA from other tissues for ova deposition or preferential dietary incorporation of PUFA into ova. These results will add to the limited FA information available in white bass and enable nutritionists to formulate broodstock diets that maximize reproductive potential in this species.

Genetic architecture of sex determination in fish: applications to sex ratio control in aquaculture

PubMed Central

Martínez, Paulino; Viñas, Ana M.; Sánchez, Laura; Díaz, Noelia; Ribas, Laia; Piferrer, Francesc

2014-01-01

Controlling the sex ratio is essential in finfish farming. A balanced sex ratio is usually good for broodstock management, since it enables to develop appropriate breeding schemes. However, in some species the production of monosex populations is desirable because the existence of sexual dimorphism, primarily in growth or first time of sexual maturation, but also in color or shape, can render one sex more valuable. The knowledge of the genetic architecture of sex determination (SD) is convenient for controlling sex ratio and for the implementation of breeding programs. Unlike mammals and birds, which show highly conserved master genes that control a conserved genetic network responsible for gonad differentiation (GD), a huge diversity of SD mechanisms has been reported in fish. Despite theory predictions, more than one gene is in many cases involved in fish SD and genetic differences have been observed in the GD network. Environmental factors also play a relevant role and epigenetic mechanisms are becoming increasingly recognized for the establishment and maintenance of the GD pathways. Although major genetic factors are frequently involved in fish SD, these observations strongly suggest that SD in this group resembles a complex trait. Accordingly, the application of quantitative genetics combined with genomic tools is desirable to address its study and in fact, when applied, it has frequently demonstrated a multigene trait interacting with environmental factors in model and cultured fish species. This scenario has notable implications for aquaculture and, depending upon the species, from chromosome manipulation or environmental control techniques up to classical selection or marker assisted selection programs, are being applied. In this review, we selected four relevant species or fish groups to illustrate this diversity and hence the technologies that can be used by the industry for the control of sex ratio: turbot and European sea bass, two reference species of the European aquaculture, and salmonids and tilapia, representing the fish for which there are well established breeding programs. PMID:25324858

An overview of cleaner fish use in Ireland.

PubMed

Bolton-Warberg, M

2017-11-21

Sea lice infestations represent one of the most significant challenges facing the salmon farming industry, giving rise to lost production, additional costs of treatment and potential negative interactions with wild stocks. At present, cleaner fish, which actively remove lice from salmon, are an effective, biological, long-term option which has been adopted by many countries. In Ireland, several key studies were conducted in the 1990s on the use of wild-caught wrasse (corkwing, goldsinny and rock cook) as cleaner fish in experimental and commercial scale trials. More recently, the National University of Ireland Galway (NUIG), at their marine research facility in Carna (CRS), has undertaken applied research on ballan wrasse and lumpsucker. Currently, CRS is providing lumpsucker juveniles and research and development for the Irish salmon industry with support from BIM (Ireland's Seafood Development Agency) and Marine Harvest Ireland. There is a large amount of research currently being carried out in this area in all countries that are utilizing cleaner fish technology. The current focus in Ireland is the development of a native lumpsucker broodstock to facilitate its sustainable production. The aim of this article was to provide an overview of the research, challenges and use of cleaner fish in Ireland. © 2017 The Authors Journal of Fish Diseases Published by John Wiley & Sons Ltd.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berejikian, Barry A.

The success of captive broodstock programs depends on high in-culture survival, appropriate development of the reproductive system, and the behavior and survival of cultured salmon after release, either as adults or juveniles. Continuing captive broodstock research designed to improve technology is being conducted to cover all major life history stages of Pacific salmon. Accomplishments detailed in this report are listed below by major objective. Objective 1: This study documented that captively reared Chinook exhibited spawn timing similar to their founder anadromous population. An analysis of spawn timing data of captively reared Chinook salmon that had received different levels of antibioticmore » treatment did not suggest that antibiotic treatments during the freshwater or seawater phase of the life cycle affects final maturation timing. No effect of rearing density was found with respect to spawn timing or other reproductive behaviors. Objective 2: This study investigated the critical period(s) for imprinting for sockeye salmon by exposing juvenile salmon to known odorants at key developmental stages. Molecular assessments of imprinting-induced changes in odorant receptor gene expression indicated that regulation of odorant expression differs between coho and sockeye salmon. While temporal patterns differ between these species, exposure to arginine elicited increases in odorant receptor mRNA expression in sockeye salmon. Objective 3: This study: (i) identified the critical period when maturation is initiated in male spring Chinook salmon and when body growth affects onset of puberty, (ii) described changes in the reproductive endocrine system during onset of puberty and throughout spermatogenesis in male spring Chinook salmon, (iii) found that the rate of oocyte development prior to vitellogenesis is related to body growth in female spring Chinook, and (iv) demonstrated that growth regimes which reduce early (age 2) male maturation slow the rate of primary and early secondary oocyte growth, but do not alter number of oocytes at these stages of development. Objective 4 : This study, (1) determined that infected fish treated with oxytetracycline-medicated feed (as fry or as presmolts) had improved survival compared to nonmedicated fish, (2) determined that a single 14-day course of oral azithromycin at first feeding or at the start of smoltification is sufficient for significant azithromycin retention in internal tissues for at least a year, and (3) established that Renibacterium salmoninarum with an azithromycin-resistant phenotype can be isolated from Chinook salmon receiving macrolide antibiotic treatment. Objective 5: This study determined that for Chinook salmon rearing in similar, 'common environment' regimes in seawater, control fish have survived at a higher rate since seawater transfer than have experimentally inbred fish. However, in all groups, the variation among families in survival has been substantial, ranging from 0% to 100% over the entire year and from 0% to 40% since seawater transfer. The highly significant effect of variation among families within both stocks indicates that substantial genetic variation for size remains in these populations.« less

Domestication of Mahseer (Tor soro) in Indonesia.

PubMed

Gustiano, R; Kontara, E K; Wahyuningsih, H; Subagja, J; Asih, S; Saputr, A

2013-01-01

Mahseer is an economically important fish in the world with a distribution from the Himalayan Mountains up to Southeast Asia. One species of Masheer is Tor soro in the Indonesian Archipelago. For a long time, T. soro has been collected from the wild and now is becoming rare. In addition, the degradation of its habitat is another problem. Hence, it is urgent that this species is saved from extinction. Domestication of T. soro from North Sumatra has been conducted since 1996. This paper reports the results of domestication of T. soro starting from collecting live specimens, genetic and morphometric characterizations, evaluation to breeding, and growing out. In 2010, domestication was successful to produce a second generation of young broodstocks and now the breeding technology is being shared with several provinces where T. soro exist.

Evaluation of substitute diets for live algae in the captive maintenance of adult and subadult unionidae

USGS Publications Warehouse

Nichols, S. Jerrine; Garling, D.

2002-01-01

Ten nonlive algal diets were evaluated as potential broodstock diets for adult and subadult unionids. These diets varied significantly in their ability to support growth, reproduction and survival. Growth, increase in glycogen stores, and limited glochidial formation were seen in most unionid species on two of the diets. However, long-term survival (>3 y) remained problematic, and the cause of mortality in these animals could not be determined. While two of the diets tested are potentially useful for supplemental feeding of adult unionids to increase glycogen levels during quarantine, or during short-term captive maintenance in the laboratory, none can be recommended without reservation for long-term maintenance because of the lack of survival after three years during this study.

Interpreting dual ELISA and qPCR data for bacterial kidney disease of salmonids.

PubMed

Nance, Shelly L; Riederer, Michael; Zubkowski, Tyler; Trudel, Marc; Rhodes, Linda D

2010-09-02

Although there are a variety of methods available for the detection of Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmon and trout, the enzyme-linked immunosorbent assay (ELISA) is probably the most widely used method. However, ELISA measures bacterial antigen, which does not necessarily reflect the number of cells present. We hypothesized that dual analysis of kidney tissue by ELISA and a quantitative real-time polymerase chain reaction assay (qPCR) would provide complementary information about antigen level and the number of bacterial genomes. We found that DNA extracted from the insoluble fraction of the ELISA tissue preparation produced the same qPCR result as DNA extracted directly from frozen tissue, permitting true dual analysis of the same tissue sample. We examined kidney tissue in this manner from individual free-ranging juvenile Chinook salmon and antibiotic-treated captive subadult Chinook salmon and observed 3 different patterns of results. Among the majority of fish, there was a strong correlation between the ELISA value and the qPCR value. However, subsets of fish exhibited either low ELISA values with elevated qPCR values or higher ELISA values with very low qPCR values. These observations suggest a conceptual model that allows inferences about the state of infection of individual fish based on dual ELISA/qPCR results. Although this model requires further assessment through experimental infections and treatments, it may have utility in broodstock selection programs that currently apply egg-culling practices based on ELISA alone.

Size selection from fishways and potential evolutionary responses in a threatened Atlantic salmon population

USGS Publications Warehouse

Maynard, George A.; Kinnison, M.T.; Zydlewski, Joseph D.

2017-01-01

The evolutionary effects of harvest on wild fish populations have been documented around the world; however, sublethal selective pressures can also cause evolutionary changes in phenotypes. For migratory fishes, passage facilities may represent instances of nonlethal selective pressure. Our analysis of 6 years of passage data suggests that certain fish passage facilities on the Penobscot River have been exerting selective pressure against large-bodied, anadromous Atlantic salmon (Salmo salar). At the second and third dams in the river, a 91-cm salmon was 21%–27% and 12%–16% less likely to pass than a 45-cm salmon, respectively. Fish size positively influences egg survival and number and is a heritable trait. Therefore, in a wild-reproducing population, exclusion of large fish from spawning areas may have population-level impacts. In the Penobscot River, most returning adults derive from a hatchery program that collects its broodstock after passing the first dam in the river. Analysis of fork lengths of salmon returning to the Penobscot River from 1978 to 2012 provided mixed support for evolution of size at maturity in different age classes in a pattern that may be expected from interactions with conservation hatchery operations. Additionally, slow-maturing and iteroparous individuals that represent the largest salmon size classes were essentially lost from the population during that time, and Penobscot River fish have shorter fork lengths at maturity than Atlantic salmon in undammed systems.

Sherman Creek Hatchery; Washington Department of Fish and Wildlife Fish Program, 2003 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lovrak, Jon; Combs, Mitch

2004-01-01

Sherman Creek Hatchery's primary objective is the restoration and enhancement of the recreational and subsistence fishery in Lake Roosevelt and Banks Lake. The Sherman Creek Hatchery (SCH) was designed to rear 1.7 million kokanee fry for acclimation and imprinting during the spring and early summer. Additionally, it was designed to trap all available returning adult kokanee during the fall for broodstock operation and evaluation. Since the start of this program, the operations on Lake Roosevelt have been modified to better achieve program goals. The Washington Department of Fish and Wildlife, Spokane Tribe of Indians and the Colville Confederated Tribes formmore » the interagency Lake Roosevelt Hatcheries Coordination Team (LRHCT) which sets goals and objectives for both Sherman Creek and the Spokane Tribal Hatchery. The LRHCT also serves to coordinate enhancement efforts on Lake Roosevelt and Banks Lake. Since 1994 the kokanee fingerling program has changed to yearling releases. By utilizing both the hatcheries and additional net pens, up to 1,000,000 kokanee yearlings can be reared and released. The construction and operation of twenty net pens in 2001 enabled the increased production. Another significant change has been to rear up to 300,000 rainbow trout fingerling at SCH from July through October, for stocking into the volunteer net pens. This enables the Spokane Tribal Hatchery (STH) to rear additional kokanee to further the enhancement efforts on Lake Roosevelt. Current objectives include increased use of native tributary stocks where available for propagation into Upper Columbia River Basin waters. The Lake Roosevelt Fisheries Evaluation Program (LRFEP) is responsible for monitoring and evaluation on the Lake Roosevelt Projects. From 1988 to 1998, the principal sport fishery on Lake Roosevelt has shifted from walleye to include rainbow trout and kokanee salmon (Underwood et al. 1997, Tilson and Scholz 1997). The angler use, harvest rates for rainbow and kokanee and the economic value of the fishery has increased substantially during this 10-year period. The investigations on the lake also suggest that the hatchery and net pen programs have enhanced the Lake Roosevelt fishery while not negatively impacting wild and native stocks within the lake. The 2003 Fourth Annual Two Rivers Trout Derby was again a great success. The harvest and data collection were the highest level to date with 1,668 rainbow trout and 416 kokanee salmon caught. The fishermen continue to praise the volunteer net pen program and the hatchery efforts as 90% of the rainbows and 93% of the kokanee caught were of hatchery origin (Lee, 2003).« less

Sex steroid levels, oocyte maturation and spawning performance in Waigieu seaperch (Psammoperca waigiensis) exposed to thyroxin, human chorionic gonadotropin, luteinizing hormone releasing hormone and carp pituitary extract.

PubMed

Pham, Hung Quoc; Nguyen, Anh Tuong; Nguyen, Mao Dinh; Arukwe, Augustine

2010-02-01

In the present study, we have investigated the sex steroid hormone levels, oocyte maturation and spawning performance in Waigieu seaperch (Psammoperca waigiensis) exposed to different doses (0, (control), 0.05, 0.25 and 0.5 mg/kg fish) of thyroxin (T(4)) both through diet (continuously) and injection (single injection). In addition, we also studied plasma steroid hormone levels and spawning performances in female fish injected with a single dose of D-Ala(6), Pro(9)-Net-mGnRH (LHRHa: 50 microg/kg), human chronic gonadotropin (HCG: 1,500 IU/kg) and carp pituitary extract (CPE: 10 mg/kg). In all experiments, samples were collected at 0, 6, 12, 24 and 48 h after exposure. T4 exposure via dietary route produced differential and enhanced effects, compared with when the compound was injected to the broodstock. A significant association between exposure to dietary T4, elevated plasma steroid hormone levels, maturation-, spawning-, fertilization- and hatching rate, egg diameter, embryogenesis and larval growth were observed. Interestingly, we observed that broodstock groups fed with T4 doses spawned 20 days earlier than the control group. Thus, we propose that these differences may be attributed to higher systemic availability of T4 due to dietary exposure that is easily transferable to eggs and embryos, as opposed to injection that require absorption to increase bioavailability. Furthermore, our results show that LHRHa, CPE and HCG produced significant increase in spawning rate, but significantly reduced fertilization- and hatching rates. Waigieu seaperch is a new candidate for marine aquaculture in Vietnam and relatively little is known about the reproductive biology and endocrinology of this species. Therefore, the present study forms an integral basis for understanding the reproductive endocrinology of a tropical marine finfish with increasing aquaculture prospects and may also contribute in the development of sustainable aquaculture of this species in a developing country such as Vietnam. 2009 Elsevier Inc. All rights reserved.

Broodstock management and hormonal manipulations of fish reproduction.

PubMed

Mylonas, Constantinos C; Fostier, Alexis; Zanuy, Silvia

2010-02-01

Control of reproductive function in captivity is essential for the sustainability of commercial aquaculture production, and in many fishes it can be achieved by manipulating photoperiod, water temperature or spawning substrate. The fish reproductive cycle is separated in the growth (gametogenesis) and maturation phase (oocyte maturation and spermiation), both controlled by the reproductive hormones of the brain, pituitary and gonad. Although the growth phase of reproductive development is concluded in captivity in most fishes-the major exemption being the freshwater eel (Anguilla spp.), oocyte maturation (OM) and ovulation in females, and spermiation in males may require exogenous hormonal therapies. In some fishes, these hormonal manipulations are used only as a management tool to enhance the efficiency of egg production and facilitate hatchery operations, but in others exogenous hormones are the only way to produce fertilized eggs reliably. Hormonal manipulations of reproductive function in cultured fishes have focused on the use of either exogenous luteinizing hormone (LH) preparations that act directly at the level of the gonad, or synthetic agonists of gonadotropin-releasing hormone (GnRHa) that act at the level of the pituitary to induce release of the endogenous LH stores, which, in turn act at the level of the gonad to induce steroidogenesis and the process of OM and spermiation. After hormonal induction of maturation, broodstock should spawn spontaneously in their rearing enclosures, however, the natural breeding behavior followed by spontaneous spawning may be lost in aquaculture conditions. Therefore, for many species it is also necessary to employ artificial gamete collection and fertilization. Finally, a common question in regards to hormonal therapies is their effect on gamete quality, compared to naturally maturing or spawning broodfish. The main factors that may have significant consequences on gamete quality-mainly on eggs-and should be considered when choosing a spawning induction procedure include (a) the developmental stage of the gonads at the time the hormonal therapy is applied, (b) the type of hormonal therapy, (c) the possible stress induced by the manipulation necessary for the hormone administration and (d) in the case of artificial insemination, the latency period between hormonal stimulation and stripping for in vitro fertilization. Copyright 2009 Elsevier Inc. All rights reserved.

Paper-based archiving of biological samples from fish for detecting betanodavirus.

PubMed

Navaneeth Krishnan, A; Bhuvaneswari, T; Ezhil Praveena, P; Jithendran, K P

2016-07-01

This study was carried out to evaluate the efficiency of the Flinders Technology Associates (FTA(®)) card (Whatman(®)) as a sampling device and storage platform for RNA from betanodavirus-infected biological samples (viz., larvae, broodstock, cell culture supernatants and rearing seawater spiked with infected materials). The study showed that FTA cards can be used to detect betanodaviruses by reverse transcription-polymerase chain reaction (RT-PCR). The diagnostic efficiency of RT-PCR from all sample types on FTA cards decreased after 21 days of storage at 4 °C, although the virus could be detected up to 28 days by nested RT-PCR. The FTA card protocol thus provides a supplementary method for quick and easy collection of samples, preservation of RNA on a dry storage basis, and detection of betanodavirus-infected fish.

Management of bacterial kidney disease in Chinook Salmon hatcheries based on broodstock testing by enzyme-linked immunosorbent assay: A multiyear study

USGS Publications Warehouse

Munson, A. Douglas; Elliott, Diane G.; Johnson, Keith

2010-01-01

From the mid-1980s through the early 1990s, outbreaks of bacterial kidney disease (BKD) caused by Renibacterium salmoninarum continued in Chinook salmon Oncorhynchus tshawytscha in Idaho Department of Fish and Game (IDFG) hatcheries despite the use of three control methods: (1) injection of returning adult fish with erythromycin to reduce prespawning BKD mortality and limit vertical transmission of R. salmoninarum, (2) topical disinfection of green eggs with iodophor, and (3) prophylactic treatments of juvenile fish with erythromycin-medicated feed. In addition, programs to manage BKD through measurement of R. salmoninarum antigen levels in kidney tissues from spawning female Chinook salmon by an enzyme-linked immunosorbent assay (ELISA) were tested over 13–15 brood years at three IDFG hatcheries. The ELISA results were used for either (1) segregated rearing of progeny from females with high ELISA optical density (OD) values (usually ≥0.25), which are indicative of high R. salmoninarum antigen levels, or (2) culling of eggs from females with high ELISA OD values. The ELISA-based culling program had the most profound positive effects on the study populations. Mortality of juvenile fish during rearing was significantly lower at each hatchery for brood years derived from culling compared with brood years for which culling was not practiced. The prevalence of R. salmoninarum in juvenile fish, as evidenced by detection of the bacterium in kidney smears by the direct fluorescent antibody test, also decreased significantly at each hatchery. In addition, the proportions of returning adult females with kidney ELISA OD values of 0.25 or more decreased 56–85% for fish reared in brood years during which culling was practiced, whereas the proportions of ELISA-negative adults increased 55–58%. This management strategy may allow IDFG Chinook salmon hatcheries to reduce or eliminate prophylactic erythromycin-medicated feed treatments. We recommend using ELISA-based management of BKD in Chinook salmon hatcheries where it is a concern.

Conservation Genetics of Threatened Hippocampus guttulatus in Vulnerable Habitats in NW Spain: Temporal and Spatial Stability of Wild Populations with Flexible Polygamous Mating System in Captivity

PubMed Central

López, Almudena; Vera, Manuel; Planas, Miquel; Bouza, Carmen

2015-01-01

This study was focused on conservation genetics of threatened Hippocampus guttulatus on the Atlantic coast of NW Iberian Peninsula. Information about spatial structure and temporal stability of wild populations was obtained based on microsatellite markers, and used for monitoring a captive breeding program firstly initiated in this zone at the facilities of the Institute of Marine Research (Vigo, Spain). No significant major genetic structure was observed regarding the biogeographical barrier of Cape Finisterre. However, two management units under continuous gene flow are proposed based on the allelic differentiation between South-Atlantic and Cantabrian subpopulations, with small to moderate contemporary effective size based on single-sample methods. Temporal stability was observed in South-Atlantic population samples of H. guttulatus for the six-year period studied, suggesting large enough effective population size to buffer the effects of genetic drift within the time frame of three generations. Genetic analysis of wild breeders and offspring in captivity since 2009 allowed us to monitor the breeding program founded in 2006 in NW Spain for this species. Similar genetic diversity in the renewed and founder broodstock, regarding the wild population of origin, supports suitable renewal and rearing processes to maintain genetic variation in captivity. Genetic parentage proved single-brood monogamy in the wild and in captivity, but flexible short- and long-term mating system under captive conditions, from strict monogamy to polygamy within and/or among breeding seasons. Family analysis showed high reproductive success in captivity under genetic management assisted by molecular relatedness estimates to avoid inbreeding. This study provides genetic information about H. guttulatus in the wild and captivity within an uncovered geographical range for this data deficient species, to be taken into account for management and conservation purposes. PMID:25646777

Roles of insulin-like growth factors in metamorphic development of turbot (Scophthalmus maximus).

PubMed

Jia, Yudong

2018-01-31

Larval turbot (Scophthalmus maximus) undergo metamorphosis, a late post-embryonic developmental event that precedes juvenile transition. Insulin-like growth factors (IGFs) are important endocrine/autocrine/paracrine factors that provide essential signals to control of the embryonic and postnatal development of vertebrate species, including fish. Accumulating evidence suggests that IGFs are involved in regulating the metamorphic development of flatfish. This mini review focus on the functions of all known IGFs (IGF-I and IGF-II) during the metamorphic development of turbot. Information about IGFs and insulin-like growth factors binding proteins (IGFBPs) from other teleosts is also included in this review to provide an overview of IGFs functions in the metamorphic development of turbot. These findings may enhance our understanding of the potential roles of the IGFs system in controlling of flatfish metamorphosis and contributing to the improvement of broodstock management strategies for larval turbot. Copyright © 2018 Elsevier Inc. All rights reserved.

Standardized seawater rearing of chinook salmon smolts to evaluate hatchery practices showed low statistical power

USGS Publications Warehouse

Palmisano, Aldo N.; Elder, N.E.

2001-01-01

We examined, under standardized conditions, seawater survival of chinook salmon Oncorhynchus tshawytscha at the smolt stage to evaluate the experimental hatchery practices applied to their rearing. The experimental rearing practices included rearing fish at different densities; attempting to control bacterial kidney disease with broodstock segregation, erythromycin injection, and an experimental diet; rearing fish on different water sources; and freeze branding the fish. After application of experimental rearing practices in hatcheries, smolts were transported to a rearing facility for about 2-3 months of seawater rearing. Of 16 experiments, 4 yielded statistically significant differences in seawater survival. In general we found that high variability among replicates, plus the low numbers of replicates available, resulted in low statistical power. We recommend including four or five replicates and using ?? = 0.10 in 1-tailed tests of hatchery experiments to try to increase the statistical power to 0.80.

Status and reproduction of Gulf coast strain walleye in a Tombigbee River tributary

USGS Publications Warehouse

Schramm, H.L.; Hart, J.; Hanson, L.A.

2004-01-01

Walleye (Sander vitreus [Mitchill]) are native to rivers and streams in the Mobile River basin in Mississippi and Alabama. These populations comprise a genetically unique strain (Gulf coast walleye, GCW) and represent the southernmost distribution of walleye in the United States. Luxapallila Creek was considered an important spawning site for GCW prior to and shortly after impoundment of the Tombigbee River in 1980. Extensive sampling in Luxapallila Creek in 2001 and 2002 collected only one larval walleye. Microsatellite DNA analysis suggested 14 of 16 adult walleye from Luxapallila Creek were hatchery-produced fish or their progeny. Controlled angling catch rates of adult walleye have declined since 1997. The scarcity of wild-spawned walleye and the similarity of wild-caught and hatchery broodstock walleye indicates that the GCW population in, or spawning in, Luxapallila Creek is sustained by stocking and recruitment from these stocked fish may be diminishing.

Genetic structure of Columbia River redband trout populations in the Kootenai River drainage, Montana, revealed by microsatellite and allozyme loci

USGS Publications Warehouse

Knudsen, K.-L.; Muhlfeld, C.C.; Sage, G.K.; Leary, R.F.

2002-01-01

We describe the genetic divergence among 10 populations of redband trout Oncorhynchus mykiss gairdneri from the upper Columbia River drainage. Resident redband trout from two watersheds in the Kootenai River drainage and hatchery stocks of migratory Kamloops redband trout from Kootenay Lake, British Columbia, were analyzed using allele frequency data from microsatellite and allozyme loci. The Kamloops populations have significantly different allele frequencies from those of the Kootenai River drainage. Of the total genetic variation detected in the resident redband trout, 40.7% (microsatellites) and 15.5% (allozymes) were due to differences between populations from the two Kootenai River watersheds. The divergence among populations within each watershed, however, was less than 3.5% with both techniques. Our data indicate that watershed-specific broodstocks of redband trout are needed by fisheries managers for reintroduction or the supplementation of populations at risk of extinction.

Overview of biology and aquaculture of Amur sturgeon (Acipenser schrenckii) in China

USGS Publications Warehouse

Zhuang, P.; Kynard, B.; Zhang, L.; Zhang, T.; Zhang, Z.; Li, D.

2002-01-01

Amur sturgeon, Acipenser schrenckii is a large riverine species (max. 3 m length and 190 kg weight) native to the Amur River. In the middle Amur River, males first spawn at 7-8 years of age and females at 9-10 years. Due to overfishing and habitat alteration, the abundance of wild stocks has rapidly declined in recent years. Using wild adults, artificial spawning began in the 1950s in China, and since the early 1990s Amur sturgeon has become the most popular sturgeon for aquaculture. The species is adaptive to many conditions of artificial culture, including traditional Chinese fish culture ponds, lakes, reservoirs, and cages. It will grow well on many types of food, and 1-year-old fish reared on commercial diets weigh 900-1200 g. Based on the examination of females reared for broodstock, first full sexual maturity is expected at 5-6 years of age.

Differential immune response in the hard clam (mercenaria mercenaria) against bacteria and the protistan pathogen QPX (quahog parasite unknown).

PubMed

Perrigault, Mickael; Allam, Bassem

2012-06-01

The immune response of the hard clam (quahog) Mercenaria mercenaria following challenge with live bacteria (Vibrio alginolyticus) and the protist QPX (Quahog Parasite Unknown) was investigated. The study also compared immune responses following QPX challenge in two different hard clam broodstocks exhibiting different degrees of susceptibility toward this parasite. Different immune and stress-related cellular and humoral factors were assessed including general hemocyte parameters (total and differential hemocyte counts, percentage of dead cells, reactive oxygen production, phagocytosis), parameters geared toward QPX (anti-QPX activity in plasma and hemocyte resistance to the cytotoxicity of QPX extracellular products). Two genes (ferritin and metallothionein) previously shown to be modulated following QPX exposure were molecularly characterized by rapid amplification of cDNA ends (RACE) and their transcription levels were determined in resistant and susceptible clams in response to QPX and bacterial challenge. Results indicated that both V. alginolyticus and QPX challenge triggered significant immune responses in clams with similar trends for most measured parameters. However, specific responses were observed for anti-QPX activity in plasma and hemocyte resistance to QPX products as well as ferritin and metallothionein expression according to each inoculum. Similarly, different response patterns were detected following QPX challenge in susceptible and resistant clam stocks. Resistant clams were able to elicit effective response against the parasite leading to the elimination of QPX and the restoration of constitutive immune status whereas QPX-susceptible clams triggered a strong immune modulation characterized by an acute phase response and associated acute phase protein but appeared to be less active in eliminating the parasite. These results suggest that different signaling pathways are triggered during V. alginolyticus and QPX challenge. Moreover, differences in the immune response toward QPX might be linked to the susceptibility or resistance of different clam stocks to the infection by this parasite. Copyright © 2012 Elsevier Ltd. All rights reserved.

Reintroduction of Lower Columbia River Chum Salmon into Duncan Creek, 2007 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hillson, Todd D.

2009-06-12

The National Marine Fisheries Service (NMFS) listed Lower Columbia River (LCR) chum salmon as threatened under the Endangered Species Act (ESA) in March, 1999 (64 FR 14508, March 25, 1999). The listing was in response to the reduction in abundance from historical levels of more than one-half million returning adults to fewer than 10,000 present-day spawners. Harvest, habitat degradation, changes in flow regimes, riverbed movement and heavy siltation have been largely responsible for this decline. The timing of seasonal changes in river flow and water temperatures is perhaps the most critical factor in structuring the freshwater life history of thismore » species. This is especially true of the population located directly below Bonneville Dam, where hydropower operations can block access to spawning sites, dewater redds, strand fry, cause scour or fill of redds and increase sedimentation of spawning gravels. Prior to 1997, only two chum salmon populations were recognized as genetically distinct in the Columbia River, although spawning had been documented in many Lower Columbia River tributaries. The first population was in the Grays River (RKm 34), a tributary of the Columbia River, and the second was a group of spawners utilizing the mainstem Columbia River just below Bonneville Dam (RKm 235) adjacent to Ives Island and in Hardy and Hamilton creeks. Using additional DNA samples, Small et al. (2006) grouped chum salmon spawning in the mainstem Columbia River and the Washington State tributaries into three groups: the Coastal, the Cascade and the Gorge. The Coastal group comprises those spawning in the Grays River, Skamokawa Creek and the broodstock used at the Sea Resources facility on the Chinook River. The Cascade group comprises those spawning in the Cowlitz (both summer and fall stocks), Kalama, Lewis, and East Fork Lewis rivers, with most supporting unique populations. The Gorge group comprises those spawning in the mainstem Columbia River from the I-205 Bridge up to Bonneville Dam and those spawning in Hamilton and Hardy creeks. Response to the federal ESA listing has been primarily through direct-recovery actions: reducing harvest, hatchery supplementation using local broodstock for populations at catastrophic risk, habitat restoration (including construction of spawning channels) and flow agreements to protect spawning and rearing areas. Both state and federal agencies have built controlled spawning areas. In 1998, the Washington Department of Fish and Wildlife (WDFW) began a chum salmon supplementation program using native stock on the Grays River. This program was expanded during 1999 - 2001 to include reintroduction into the Chinook River using eggs from the Grays River Supplementation Program. These eggs are incubated at the Grays River Hatchery, reared to release size at the Sea Resources Hatchery on the Chinook River, and the fry are released at the mouth of the Chinook River. Native steelhead, chum, and coho salmon are present in Duncan Creek, and are recognized as subpopulations of the Lower Gorge population, and are focal species in the Lower Columbia Fish Recovery Board (LCFRB) plan. Steelhead, chum and coho salmon that spawn in Duncan Creek are listed as Threatened under the ESA. Duncan Creek is classified by the LCFRB plan as a watershed for intensive monitoring (LCFRB 2004). This project was identified in the 2004 Federal Columbia River Power System (FCRPS) revised Biological Opinion (revised BiOp) to increase survival of chum salmon, 'BPA will continue to fund the program to re-introduce Columbia River chum salmon into Duncan Creek as long as NOAA Fisheries determines it to be an essential and effective contribution to reducing the risk of extinction for this ESU'. (USACE et al. 2004, page 85-86). The Governors Forum on Monitoring and Salmon Recovery and Watershed Health recommends one major population from each ESU have adult and juvenile monitoring. Duncan Creek chum salmon are identified in this plan to be intensively monitored. Planners recommended that a combination of natural and hatchery production would be the most likely way to produce the most rapid sustainable improvement in chum runs. Specifically, it was assumed that improving habitat conditions would promote efficient natural production, and that the most rapid way to rebuild the run would be to combine releases of an appropriate stock into the improved habitat. WDFW's recovery strategy for LCR chum salmon, as outlined in the WDFW Grays River and Washougal Hatchery Genetic Management Plans (HGMP), is as follows. First, determine if remnant populations of chum salmon exist in LCR tributaries. Second, if such populations exist, develop stock-specific recovery plans involving habitat restoration that include the creation of spawning refugias, supplementation where necessary, and a habitat and fish monitoring and evaluation plan.« less

Isolation and characterization of the fall Chinook aquareovirus

USGS Publications Warehouse

Makhsous, Negar; Jensen, Nicole L.; Haman, Katherine H.; Batts, William N.; Jerome, Keith R.; Winton, James; Greninger, Alexander L.

2017-01-01

BackgroundSalmon are paramount to the economy, ecology, and history of the Pacific Northwest. Viruses constitute one of the major threats to salmon health and well-being, with more than twenty known virus species that infect salmon. Here, we describe the isolation and characterization of the fall Chinook aquareovirus, a divergent member of the species Aquareovirus B within the family Reoviridae.MethodsThe virus was first found in 2014 as part of a routine adult broodstock screening program in which kidney and spleen tissue samples from healthy-appearing, adult fall Chinook salmon (Oncorhynchus tshawytscha) returning to a hatchery in Washington State produced cytopathic effects when inoculated onto a Chinook salmon embryo cell line (CHSE-214). The virus was not able to be confirmed by an RT-PCR assay using existing aquareovirus pan-species primers, and instead was identified by metagenomic next-generation sequencing. Metagenomic next-generation sequencing was used to recover the full genome and completed using 3′ RACE.ResultsThe genome of the fall Chinook aquareovirus contains 11 segments of double-stranded RNA totaling 23.3 kb, with each segment flanked by the canonical sequence termini found in the aquareoviruses. Sequence comparisons and a phylogenetic analysis revealed a nucleotide identity of 63.2% in the VP7 gene with the Green River Chinook virus, placing the new isolate in the species Aquareovirus B. A qRT-PCR assay was developed targeting the VP2, which showed rapid growth of the isolate during the initial 5 days in culture using CHSE-214 cells.ConclusionsThis sequence represents the first complete genome of an Aquareovirus B species. Future studies will be required to understand the potential pathogenicity and epidemiology of the fall Chinook aquareovirus.

Additive and non-additive genetic components of the jack male life history in Chinook salmon (Oncorhynchus tshawytscha).

PubMed

Forest, Adriana R; Semeniuk, Christina A D; Heath, Daniel D; Pitcher, Trevor E

2016-08-01

Chinook salmon, Oncorhynchus tshawytscha, exhibit alternative reproductive tactics (ARTs) where males exist in two phenotypes: large "hooknose" males and smaller "jacks" that reach sexual maturity after only 1 year in seawater. The mechanisms that determine "jacking rate"-the rate at which males precociously sexually mature-are known to involve both genetics and differential growth rates, where individuals that become jacks exhibit higher growth earlier in life. The additive genetic components have been studied and it is known that jack sires produce significantly more jack offspring than hooknose sires, and vice versa. The current study was the first to investigate both additive and non-additive genetic components underlying jacking through the use of a full-factorial breeding design using all hooknose sires. The effect of dams and sires descendant from a marker-assisted broodstock program that identified "high performance" and "low performance" lines using growth- and survival-related gene markers was also studied. Finally, the relative growth of jack, hooknose, and female offspring was examined. No significant dam, sire, or interaction effects were observed in this study, and the maternal, additive, and non-additive components underlying jacking were small. Differences in jacking rates in this study were determined by dam performance line, where dams that originated from the low performance line produced significantly more jacks. Jack offspring in this study had a significantly larger body size than both hooknose males and females starting 1 year post-fertilization. This study provides novel information regarding the genetic architecture underlying ARTs in Chinook salmon that could have implications for the aquaculture industry, where jacks are not favoured due to their small body size and poor flesh quality.

Serology in Finfish for Diagnosis, Surveillance, and Research: A Systematic Review.

PubMed

Jaramillo, Diana; Peeler, Edmund J; Laurin, Emilie; Gardner, Ian A; Whittington, Richard J

2017-03-01

Historically, serological tests for finfish diseases have been underused when compared with their use in terrestrial animal health. For years the nonspecific immune response in fish was judged to make serology unreliable and inferior to the direct measurement of agent analytes. We conducted a systematic review of peer-reviewed publications that reported on the development, validation, or application of serological tests for finfish diseases. A total of 168 articles met the screening criteria; most of them were focused on salmonid pathogens (e.g., Aeromonas spp. and viral hemorrhagic septicemia virus). Before the 1980s, most publications reported the use of agglutination tests, but our review indicates that enzyme-linked immunosorbent assay (ELISA) has more recently become the dominant serological test. The main application of serological tests has been in the assessment of vaccine efficacy, with few applications for surveillance or demonstration of freedom from disease, despite the advantages of serological tests over direct detection at the population level. Nonlethal sampling, low cost, and postinfection persistence of antibodies make serological assays the test of choice in surveillance, especially of valuable broodstock. However, their adoption has been constrained by poor characterization and validation. The number of publications in our review reporting diagnostic sensitivity and specificity of serological tests in finfish was small (n = 7). Foreseeing a wider use of serological tests in the future for diagnostic end purposes, we offer recommendations for mitigating deficiencies in the development and evaluation of serological tests, including optimization, control of nonspecific reactions, informed cutoff points, diagnostic accuracy, and serological baseline studies. Achieving these goals will facilitate greater international recognition of serological testing in programs supporting aquatic animal health. Received March 21, 2016; accepted September 24, 2016.

Identification of Neosho Smallmouth Bass (Micropterus dolomieu velox) stocks for possible introduction into Grand Lake, Oklahoma

USGS Publications Warehouse

Taylor, Andrew T.; Long, James M.; Schwemm, Michael R.; Tringali, Michael D.; Brewer, Shannon K.

2016-01-01

Stocking black basses (Micropterus spp.) is a common practice used to increase angling opportunities in impoundments; however, when non-native black basses are introduced they often invade riverine habitats where they threaten the persistence of other fishes, including native black basses. Neosho Smallmouth Bass (M. dolomieu velox) is endemic to portions of the Ozark Highlands and Boston Mountains ecoregions and is threatened by introductions of non-native Smallmouth Bass (“SMB”) forms. Because of recent interest in stocking SMB into Grand Lake o’ the Cherokees, we assessed the suitability of local Neosho SMB populations as potential broodstock sources by assessing introgression with non-native SMB forms, as well as characterizing population structure and genetic diversity. The majority of Neosho SMB populations contained low, but non-negligible, genomic proportions of two genetically distinct non-native SMB forms. Introgression was highest in the Illinois River upstream of Lake Tenkiller, where Tennessee ‘lake strain’ SMB were stocked in the early 1990’s. We recovered three genetically distinct clusters of Neosho SMB at the uppermost hierarchical level of population structure: a distinct Illinois River cluster and two Grand River clusters that appear to naturally mix at some sites. Genetic diversity measures generally increased with stream size, and smaller populations with low diversity measures may benefit from immigration of novel genetic material. Overall, introgression with non-native SMB forms appears to pose a prominent threat to Neosho SMB; however, relatively intact populations of Neosho SMB exist in some Grand Lake o’ the Cherokees tributaries. Results could be used in developing a stocking program that promotes and sustains existing genetic diversity within and among Neosho SMB populations.

Fragmentation of sea bass populations in the western and eastern Mediterranean as revealed by microsatellite polymorphism.

PubMed Central

Bahri-Sfar, L; Lemaire, C; Ben Hassine, O K; Bonhomme, F

2000-01-01

We studied the genetic structure at six microsatellite loci of the Mediterranean sea bass (Dicentrarchus labrax) on 19 samples collected from different localities in the western and eastern Mediterranean basins. Significant divergence was found between the two basins. The distance tree showed two separate clusters of populations which matched well with geography, with the noticeable exception of one Egyptian sample which grouped within the western clade, a fact attributable to the introduction of aquaculture broodstock. No heterogeneity was observed within the western basin (theta = 0.0014 and n.s.). However, a significant level of differentiation was found among samples of the eastern Mediterranean (theta = 0.026 and p < 0.001). These results match with water currents but probably not with the dispersal abilities of this fish species. We thus hypothesize that selective forces are at play which limit long-range dispersal, a fact to be taken into account in the debate about speciation processes in the marine environment. PMID:10853737

Salmon restoration in the Umatilla River: A study of straying and risk containment

USGS Publications Warehouse

Hayes, M.C.; Carmichael, R.W.

2002-01-01

The use of artificial propagation may produce unexpected results and the need for risk containment. Stray chinook salmon (Oncorhynchus tshawytscha) from Umatilla River releases put the threatened Snake River stock at risk, caused conflict between two plans, altered management, and greatly increased the costs for hatchery-based restoration. Stray Umatilla returns captured or observed in the Snake River averaged more than 200 fish annually and comprised up to 26% of the escapement. The risk to the threatened population stimulated a series of containment actions, including wire tagging 2-3 million fish annually, use of acclimation ponds, altering release locations, flow enhancement, and broodstock management changes. Actions for the use of artificial propagation where straying or unexpected results are a concern include marking or tagging most or all fish, limiting the number of fish initially released, recognizing environmental variables that influence straying, ensuring that funding for risk containment is available when undesirable results occur, and recognizing that unexpected results may not be manifested or identified immediately.

Egg buoyancy variability in local populations of Atlantic cod (Gadus morhua).

PubMed

Jung, Kyung-Mi; Folkvord, Arild; Kjesbu, Olav Sigurd; Agnalt, Ann Lisbeth; Thorsen, Anders; Sundby, Svein

2012-01-01

Previous studies have found strong evidences for Atlantic cod ( Gadus morhua ) egg retention in fjords, which are caused by the combination of vertical salinity structure, estuarine circulation, and egg specific gravity, supporting small-scaled geographical differentiations of local populations. Here, we assess the variability in egg specific gravity for selected local populations of this species, that is, two fjord-spawning populations and one coastal-spawning population from Northern Norway (66-71°N/10-25°E). Eggs were naturally spawned by raised broodstocks (March to April 2009), and egg specific gravity was measured by a density-gradient column. The phenotype of egg specific gravity was similar among the three local populations. However, the associated variability was greater at the individual level than at the population level. The noted gradual decrease in specific gravity from gastrulation to hatching with an increase just before hatching could be a generic pattern in pelagic marine fish eggs. This study provides needed input to adequately understand and model fish egg dispersal.

Effects of nonlethal sea lamprey attack on the blood chemistry of lake trout

USGS Publications Warehouse

Edsall, Carol Cotant; Swink, William D.

2001-01-01

A laboratory study examined changes in the blood chemistry of field-caught and hatchery-reared lake trout Salvelinus namaycush subjected to a nonlethal attack by sea lampreys Petromyzon marinus. We measured glucose, total protein, amylase, alkaline phosphatase (ALKP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase, calcium, magnesium, triglycerides, sodium, and potassium with a Kodak Ektachem DT60 Analyzer, Ektachem DTSC Module, and the DTE Module. Mean levels of total protein, AST, ALKP, hematocrit, calcium, magnesium, and sodium decreased significantly (Pa?? 0.05), and mean levels of ALT and potassium increased significantly (Pa?? 0.05) after sea lamprey feeding. Lake trout condition (K) and hematocrit levels also decreased significantly (Pa?? 0.05) after the sea lamprey attack. Frequency distributions of eight lake trout blood chemistry variables and the hematocrit were significantly different before and after a sea lamprey attack. A second study that used hatchery lake trout broodstock measured changes in hematocrit before and after a sea lamprey attack.

Sperm motility in fish: technical applications and perspectives through CASA-Mot systems.

PubMed

Gallego, V; Asturiano, J F

2018-03-09

Although a relatively high number of sperm quality biomarkers have been reported over the years in several fish species, sperm motility is nowadays considered the best biomarker for fish spermatozoa. The first scientific reports focusing on fish sperm motility date from a century ago, but the objective assessment allowed by computer-aided sperm analysis (CASA-Mot) systems was not applied to fish species until the mid-1980s. Since then, a high number of sperm kinetic parameters from more than 170 fish species have been reported in more than 700 scientific articles, covering a wide range of topics, such as sperm physiology, sperm storage, broodstock management, the phenomenon of sperm competition, ecotoxicology and understanding the life cycle of the species. The sperm kinetic parameters provided by CASA-Mot systems can serve as powerful and useful tools for aquaculture and ecological purposes, and this review provides an overview of the major research areas in which fish sperm motility assessment by a CASA-Mot system has been used successfully.

Divergent trends in life-history traits between Atlantic salmon Salmo salar of wild and hatchery origin in the Baltic Sea.

PubMed

Vainikka, A; Kallio-Nyberg, I; Heino, M; Koljonen, M-L

2010-02-01

Four Atlantic salmon Salmo salar stocks in the Baltic Sea, varying in their breeding history, were studied for changes in life-history traits over the years 1972-1995. Total length (L(T)) at age of captured (L(TC)) fish had increased throughout the study period, partly due to increased temperature and increased L(T) at release, (L(TR)) but also due to remaining cohort effects that could represent unaccounted environmental or genetic change. Simultaneously, maturation probabilities controlled for water temperature, L(TC) and L(TR) had increased in all stocks. The least change was observed in the River Tornionjoki S. salar that was subject only to supportive stockings originating from wild parents. These results suggest a long-term divergence between semi-natural and broodstock-based S. salar stocks. Increased L(T) at age explained advanced maturation only marginally, and it remains an open question to what extent the generally increased probabilities to mature at early age reflected underlying genetic changes.

Female maturation, egg characteristics and fatty acids profile in the seahorse Hippocampus guttulatus.

PubMed

Planas, M; Quintas, P; Chamorro, A; Silva, C

2010-10-01

Knowledge of the biology and ecology of seahorses (Hippocampus spp.) is scarce, but has been increasing in recent years due to their conservation status. Captivity breeding programmes can be a valuable source of information on the reproductive biology of seahorses. A captive broodstock of Hippocampus guttulatus Cuvier 1829 was established in 2006 and kept under natural-like photoperiod and temperature. Female maturation was studied during the whole reproductive season in 2007. Most egg clutches were released from May (17°C; 15L:9D) to October (18°C; 13L:11D), with peak releases occurring in June-August (20°C; 16L:8D-14L:10D). Throughout the study, four egg morphotypes were found; two regression equations were proposed for estimating egg/yolk volume based on measurements of egg and yolk biometrics. Female weight was positively correlated with yolk volume/egg volume ratio (Y(v)/E(v)) (r(s)=0.523, n=21, P<0.05) but not with E(v) or Y(v). Egg dry weight (567±141μg) was correlated with Y(v) (r(s)=0.384, n=31, P<0.05). Mean clutch size and clutch biomass were 242±142 eggs and 137±87mg dry weight, respectively. Clutch size was positively correlated to female weight (r(s)=0.479, n=25, P<0.05). Inter-clutch intervals (days) were affected by temperature (°C) as described by the following equation: Interval=357.55e(-0.1283 Temp). Estimated inter-clutch intervals at 16, 18 and 20°C were 45.9, 35.5 and 27.5 days, respectively. Egg total lipids accounted for 31.9±3.1% dry weight. Absolute lipid content in eggs was correlated with egg dry weight (r(s)=0.907, n=41, P<0.001) and Y(v) (r(s)=0.384, n=41, P<0.5). In decreasing order of relative percentage, the most important fatty acids, were 18:1n9, 16:0, 18:2n6, 20:5n3, 18:0 and 22:6n-3. The level of n-3 HUFA was 18.5±0.7% (38.4±3.3mg/g dry weight). The profile of fatty acids in eggs resembled that displayed by the broodstock diet (enriched adult Artemia). Copyright © 2010 Elsevier B.V. All rights reserved.

Influence of food restriction on the reproduction and larval performance of matrinxã, Brycon amazonicus (Spix and Agassiz, 1829).

PubMed

Camargo, A C S; Urbinati, E C

2008-11-01

This work evaluated the effect of food restriction and refeeding of matrinxã females, Brycon amazonicus, on their reproductive performance and on the growth and survival of the progeny. Broodstocks were distributed in 8 earthen tanks (15 fish/tank) and fish from 4 tanks were fed daily (G1) while fish from the other 4 tanks were fed for 3 days and not fed for 2 days (G2) during 6 months prior to artificial spawning. Among the induced females, 57% in G1 group and 45% in G2 group spawned and the mean egg weights were 208.1 g (G1) and 131.6 g (G2). Oocytes of G2 fish were smaller (1.017 +/- 0.003 mm) than oocytes of G1 fish (1.048 +/- 0.002 mm). Fertilization (71.91 +/- 12.6% and 61.18 +/- 13.7%) and hatching (61.28 +/- 33.9% and 67.50 +/- 23.4%) rates did not differ between G1 and G2 fish. Larvae were collected at hatching and at 24, 48 and 72 hours of incubation and fixed for growth measurement. After incubation, fry were transferred to aquaria and sampled 1, 5, 9 and 15 days later. G1 and G2 larvae had similar weight (1.51 +/- 0.15 and 1.46 +/- 0.07 mg) but the G2 length was significantly higher (6.26 +/- 0.13 and 6.74 +/- 0.14 mm). By the ninth day of rearing, G2 fry had higher weight (13.6 +/- 0.26 and 18.9 +/- 0.07 mg) and length (11.8 +/- 0.09 and 14.5 +/- 0.04 mm) but by the fifteenth day, G1 fry had higher weight (90.2 +/- 1.19 and 68.6 +/- 0.77 mg) and length (18.8 +/- 0.16 and 18.5 +/- 0.04 mm) than G2 fry. By the ninth day of rearing, when fry are recommended to be transferred to outdoor tanks, G2 fry were larger and after 15 days, fry produced by restricted-fed females showed higher survival. The survival rate of G2 progeny by the fifteenth day was significantly higher (24.7 +/- 2.07%) than that of G1 progeny (19.2 +/- 1.91%). The ration restriction (35% reduction) imposed on matrinxã broodstock during 6 months prior to spawning reduced the number of spawned females and the egg amount, but it did not affect fertilization and hatching rates. Otherwise restricted-female larvae were larger and presented higher survival.

Snake River Sockeye Salmon Habitat and Limnological Research : 2008 Annual Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kohler, Andre E.; Griswold, Robert G.; Taki, Doug

2009-07-31

In March 1990, the Shoshone-Bannock Tribes petitioned the National Marine Fisheries Service (NMFS) to list Snake River sockeye salmon (Oncorhynchus nerka) as endangered. Snake River sockeye salmon were officially listed as endangered in November 1991 under the Endangered Species Act (56 FR 58619). In 1991, the Snake River Sockeye Salmon Habitat and Limnological Research Project was implemented. This project is part of an interagency effort to prevent the extinction of the Redfish Lake stock of Snake River sockeye salmon. The Shoshone-Bannock Tribal goal for this project is two tiered: the immediate goal is to increase the population of Snake Rivermore » sockeye salmon while preserving the unique genetic characteristics of the evolutionarily significant unit (ESU). The Tribes long term goal is to maintain a viable population that warrants delisting and provides Tribal harvest opportunities. The Bonneville Power Administration (BPA) provides funding for this interagency Recovery effort. Collaborators in the recovery effort include the National Oceanic and Atmospheric Administration (NOAA), the Idaho Department of Fish and Game (IDFG), the University of Idaho (UI), and the Shoshone-Bannock Tribes (SBT). This report summarizes activities conducted by Shoshone-Bannock Tribal Fisheries Department personnel during the 2008 calendar year. Project tasks include: (1) monitor limnological parameters of the Sawtooth Valley lakes to assess lake productivity; (2) conduct lake fertilization in Pettit and Alturas lakes; (3) reduce the number of mature kokanee salmon spawning in Alturas Lake Creek; (4) monitor, enumerate, and evaluate sockeye salmon smolt migration from Pettit and Alturas lakes; (5) monitor spawning kokanee salmon escapement and estimate fry recruitment in Fishhook and Alturas Lake creeks; (6) conduct sockeye and kokanee salmon population surveys; (7) evaluate potential competition and predation between stocked juvenile sockeye salmon and a variety of fish species in Redfish, Pettit, and Alturas lakes; and (8) assist IDFG with captive broodstock production activities.« less

Cryopreservation of Sperm from the Endangered Colorado Pikeminnow

USGS Publications Warehouse

Tiersch, T.R.; Figiel, C.R.; Wayman, W.R.; Williamson, J.H.; Gorman, O.T.; Carmichael, G.J.

2004-01-01

We developed methods for the cryopreservation of sperm of the endangered Colorado pikeminnow Ptychocheilus lucius. Sperm were collected from a captive broodstock population of Colorado pikeminnow reared and maintained at the Dexter National Fish Hatchery and Technology Center. Our objectives were to (1) evaluate the effects on sperm motility of 24-h storage in Hanks' balanced salt solution (HBSS); (2) characterize sperm motility and duration; (3) examine the relationship between sperm motility and osmotic pressure; (4) examine the effect of four cryoprotectants (dimethyl sulfoxide [DMSO], dimethyl acetamide [DMA], glycerol, and methanol [MeOH] at two concentrations [5% and 10%]) on postthaw motility; and (5) compare the effect of two cooling rates (40??C/ min and 4??C/min) on postthaw motility. The sperm samples diluted with HBSS retained higher motility (mean ??SD, 77 ?? 22%; n = 9) than did undiluted samples (12 ?? 30%; n = 9) after 24 h of storage. When exposed to HBSS at 274 mosmols/kg or more, few sperm became motile (???1%). Exposure to HBSS at 265 mosmols/kg elicited threshold activation (defined as 10% motility), and maximum motility (>95%) was observed at 93 mosmols/ kg. The maximum motility of sperm was observed within 10 s after activation with deionized water, and sperm remained motile for 57 s. The sperm that were cooled at a rate of 40??C/min and cryopreserved with 5% MeOH retained higher postthaw motility (56 ?? 13%) than did sperm cryopreserved with DMSO, DMA, or glycerol (at 5% and 10%). When the sperm samples were cooled at a rate of 4??C/min, sperm cryopreserved with MeOH (5% or 10%) or DMSO (5% or 10%) retained the highest postthaw motilities (???14%). The use of cryopreserved sperm can assist hatchery managers in the production of fish, provide for the long-term conservation of genetic resources, and assist in the recovery of endangered species such as the Colorado pikeminnow.

Disease and health management in Asian aquaculture.

PubMed

Bondad-Reantaso, Melba G; Subasinghe, Rohana P; Arthur, J Richard; Ogawa, Kazuo; Chinabut, Supranee; Adlard, Robert; Tan, Zilong; Shariff, Mohamed

2005-09-30

Asia contributes more than 90% to the world's aquaculture production. Like other farming systems, aquaculture is plagued with disease problems resulting from its intensification and commercialization. This paper describes the various factors, providing specific examples, which have contributed to the current disease problems faced by what is now the fastest growing food-producing sector globally. These include increased globalization of trade and markets; the intensification of fish-farming practices through the movement of broodstock, postlarvae, fry and fingerlings; the introduction of new species for aquaculture development; the expansion of the ornamental fish trade; the enhancement of marine and coastal areas through the stocking of aquatic animals raised in hatcheries; the unanticipated interactions between cultured and wild populations of aquatic animals; poor or lack of effective biosecurity measures; slow awareness on emerging diseases; the misunderstanding and misuse of specific pathogen free (SPF) stocks; climate change; other human-mediated movements of aquaculture commodities. Data on the socio-economic impacts of aquatic animal diseases are also presented, including estimates of losses in production, direct and indirect income and employment, market access or share of investment, and consumer confidence; food availability; industry failures. Examples of costs of investment in aquatic animal health-related activities, including national strategies, research, surveillance, control and other health management programmes are also provided. Finally, the strategies currently being implemented in the Asian region to deal with transboundary diseases affecting the aquaculture sector are highlighted. These include compliance with international codes, and development and implementation of regional guidelines and national aquatic animal health strategies; new diagnostic and therapeutic techniques and new information technology; new biosecurity measures including risk analysis, epidemiology, surveillance, reporting and planning for emergency response to epizootics; targeted research; institutional strengthening and manpower development (education, training and extension research and diagnostic services).

Optimal Site Characterization and Selection Criteria for Oyster Restoration using Multicolinear Factorial Water Quality Approach

NASA Astrophysics Data System (ADS)

Yoon, J.

2015-12-01

Elevated levels of nutrient loadings have enriched the Chesapeake Bay estuaries and coastal waters via point and nonpoint sources and the atmosphere. Restoring oyster beds is considered a Best Management Practice (BMP) to improve the water quality as well as provide physical aquatic habitat and a healthier estuarine system. Efforts include declaring sanctuaries for brood-stocks, supplementing hard substrate on the bottom and aiding natural populations with the addition of hatchery-reared and disease-resistant stocks. An economic assessment suggests that restoring the ecological functions will improve water quality, stabilize shorelines, and establish a habitat for breeding grounds that outweighs the value of harvestable oyster production. Parametric factorial models were developed to investigate multicolinearities among in situ water quality and oyster restoration activities to evaluate posterior success rates upon multiple substrates, and physical, chemical, hydrological and biological site characteristics to systematically identify significant factors. Findings were then further utilized to identify the optimal sites for successful oyster restoration augmentable with Total Maximum Daily Loads (TMDLs) and BMPs. Factorial models evaluate the relationship among the dependent variable, oyster biomass, and treatments of temperature, salinity, total suspended solids, E. coli/Enterococci counts, depth, dissolved oxygen, chlorophyll a, nitrogen and phosphorus, and blocks consist of alternative substrates (oyster shells versus riprap, granite, cement, cinder blocks, limestone marl or combinations). Factorial model results were then compared to identify which combination of variables produces the highest posterior biomass of oysters. Developed Factorial model can facilitate maximizing the likelihood of successful oyster reef restoration in an effort to establish a healthier ecosystem and to improve overall estuarine water quality in the Chesapeake Bay estuaries.

Simulated effects of YY-male stocking and manual suppression for eradicating nonnative Brook Trout populations

USGS Publications Warehouse

Schill, Daniel J.; Meyer, Kevin A.; Hansen, Michael J.

2017-01-01

Eradication of nonnative Brook Trout Salvelinus fontinalis populations is difficult to achieve with standard techniques, such as electrofishing removal or piscicides; new approaches are needed. A novel concept is to stock “supermale” hatchery fish with wild conspecifics. Supermales (MYY) have two Y-chromosomes, resulting in offspring that are all males; over time, successful supermale reproduction could eradicate the wild population. We constructed an age-structured stochastic model to investigate the effects of manually suppressing wild fish and stocking MYY fingerlings on the long-term viability of hypothetical nonnative Brook Trout populations. In streams, an annual stocking rate of supermales equivalent to 50% of wild age-0 Brook Trout density combined with an annual selective suppression rate equivalent to 50% of wild Brook Trout density resulted in a time to extirpation of only 2–4 years if supermale fitness was equivalent to wild male fitness. However, time to extirpation in streams was 5–15 years if supermale fitness was 80% lower than wild male fitness. In alpine lakes, higher supermale stocking rates and nonselective gillnetting were required to eradicate Brook Trout populations. If supermales were assumed to be as fit as wild males, however, any supermale stocking rate greater than 49% in alpine lakes or 60% in streams achieved eradication in 10 years or less, regardless of the suppression rate. Because manual suppression and the stocking of MYY fingerlings can readily be conducted at the levels assumed in our simulations, use of such an integrated pest management (IPM) approach could extirpate undesirable Brook Trout populations within reasonably short periods of time. Given the recent successful development of an MYY Brook Trout broodstock capable of producing large numbers of MYY fingerlings and given the positive results of the present simulations for both streams and alpine lakes, field testing of MYY stocking is warranted within an IPM program that includes manual suppression for eradicating undesirable Brook Trout populations.

Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits

PubMed Central

Sánchez-Ramos, Irma; Cross, Ismael; Mácha, Jaroslav; Martínez-Rodríguez, Gonzalo; Krylov, Vladimir; Rebordinos, Laureana

2012-01-01

Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gilthead seabream (Sparus aurata): the growth hormone (GH), insulin-like growth factor-1 (IGF-1), myostatin (MSTN-1), prolactin (PRL), and somatolactin (SL) genes. Specimens evaluated were from a commercial broodstock comprising 131 breeders (from which 36 males and 44 females contributed to the progeny). In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, were analyzed; tests were made for significant associations between these markers and growth traits. ANOVA results showed a significant association between MSTN-1 gene polymorphism and growth traits. Pairwise tests revealed several RFLPs in the MSTN-1 gene with significant heterogeneity of genotypes among size groups. PRL and MSTN-1 genes presented linkage disequilibrium. The MSTN-1 gene was mapped in the centromeric region of a medium-size acrocentric chromosome pair. PMID:22666112

Adaptive genetic markers discriminate migratory runs of Chinook salmon (Oncorhynchus tshawytscha) amid continued gene flow

PubMed Central

O'Malley, Kathleen G; Jacobson, Dave P; Kurth, Ryon; Dill, Allen J; Banks, Michael A

2013-01-01

Neutral genetic markers are routinely used to define distinct units within species that warrant discrete management. Human-induced changes to gene flow however may reduce the power of such an approach. We tested the efficiency of adaptive versus neutral genetic markers in differentiating temporally divergent migratory runs of Chinook salmon (Oncorhynchus tshawytscha) amid high gene flow owing to artificial propagation and habitat alteration. We compared seven putative migration timing genes to ten microsatellite loci in delineating three migratory groups of Chinook in the Feather River, CA: offspring of fall-run hatchery broodstock that returned as adults to freshwater in fall (fall run), spring-run offspring that returned in spring (spring run), and fall-run offspring that returned in spring (FRS). We found evidence for significant differentiation between the fall and federally listed threatened spring groups based on divergence at three circadian clock genes (OtsClock1b, OmyFbxw11, and Omy1009UW), but not neutral markers. We thus demonstrate the importance of genetic marker choice in resolving complex life history types. These findings directly impact conservation management strategies and add to previous evidence from Pacific and Atlantic salmon indicating that circadian clock genes influence migration timing. PMID:24478800

Population genetics of the black scar oyster, Crassostrea iredalei: repercussion of anthropogenic interference.

PubMed

Zainal Abidin, Danial Hariz; Mustaffa, Suzana; Rahim, Masazurah A; Nair, Devakie M; Naim, Darlina Md; Mohd Nor, Siti Azizah

2016-01-01

Mitochondrial cytochrome oxidase subunit I (COI) gene was utilized to assess the population genetics of the commercially important black scar oyster, Crassostrea iredalei among 11 populations throughout the west and east coasts Peninsular Malaysia and Sabah (Malaysian Borneo). Overall, populations of C. iredalei demonstrated low nucleotide diversity π (0.000-0.004) and low-to-high haplotype diversity h (0.000-0.795) levels. Genetic structuring was detected between the Peninsular Malaysia and Sabah populations as revealed by the FST analysis. However, the COI gene analyses showed minimal and non-significant (p > 0.05) population differentiation within the east and west coasts Peninsular Malaysia and Sabah regions. This was attributed to both high larval dispersal along the east and west coasts and human-driven spat translocation between the two coastlines due to C. iredalei cultivation practices. Phylogeographic relationships inferences were also conducted to further support these hypotheses. The neutrality and mismatch distribution analyses suggested that C. iredalei had experienced a/several bottleneck event(s), followed by population expansion. The molecular information obtained from this study could be incorporated in a pragmatic aquaculture management strategy of wild broodstock and the hatchery lines of C. iredalei in Malaysia.

Capture, adaptation and artificial control of reproduction of Lophiosilurus alexandri: A carnivorous freshwater species.

PubMed

Costa, Deliane Cristina; de Souza e Silva, Walisson; Melillo Filho, Reinaldo; Miranda Filho, Kleber Campos; Epaminondas dos Santos, José Claudio; Kennedy Luz, Ronald

2015-08-01

The present study describes the capture adaptation and reproduction of wild Lophiosilurus alexandri broodstock in laboratory conditions. There were two periods when capturing was performed in natural habitats. The animals were placed in four tanks of 5m(3) with water temperatures at 28°C with two tanks having sand bottoms. Thirty days after the temperature increased (during the winter) the first spawning occurred naturally, but only in tanks with sand on the bottom. During the breeding season, there were 24 spawning bouts with egg mass collections occurring as a result of the spawning bouts that occurred in the tanks. The hatching rates for eggs varied from 0% to 95%. The spawning bouts were mainly at night and on weekends. In the second reproductive period, the animals were sexed by cannulation and distributed in four tanks with all animals being maintained in tanks with sand on the bottom at 28°C. During this phase, there were 36 spawning bouts. Findings in the present study contribute to the understanding of the reproductive biology of this endangered species during captivity. Copyright © 2015 Elsevier B.V. All rights reserved.

Thiamine deficiency effects on the vision and foraging ability of lake trout fry

USGS Publications Warehouse

Tillitt, Donald E.; Zajicek, James L.; Claunch, Rachel; Honeyfield, Dale C.; Fitzsimons, John D.; Brown, Scott B.

2008-01-01

The exact causes of the historical recruitment failures of Great Lakes lake trout Salvelinus namaycush are unknown. Thiamine deficiency has been associated with neurological abnormalities in lake trout that lead to early mortality syndrome (EMS) in salmonine swim-up fry, and EMS-related mortality at the swim-up stage is a factor that contributes to the reproductive failure of lake trout populations in the Great Lakes. The potential for adverse effects of thiamine deficiency beyond the swim-up stage is unknown. We investigated the effects of low egg thiamine on behavioral functions in young, post-swim-up lake trout fry. The behavioral endpoints included visual acuity and prey capture rates in the same groups of lake trout fry from each family. Low-thiamine eggs were produced by feeding lake trout broodstock diets entailing thiaminase activity. The thiamine content of the spawned eggs ranged from 0.3 to 26.1 nmol/g. Both visual acuity and prey capture rates were affected by the thiamine content of the eggs. The visual acuity of lake trout was severely affected by low egg thiamine, mainly at thiamine concentrations below the threshold of 0.8 nmol/g but also at higher concentrations in field-collected eggs. Feeding was also reduced with low egg thiamine content. The reduction of prey capture rates was dramatic below 0.8 nmol/g and less dramatic, but still significant, in a portion of the families with egg thiamine concentrations of less than 5.0 nmol/g from both laboratory and field samples. Approximately one-third of the latter families had reduced feeding rates. Deficits in visual acuity may be part of the mechanism leading to decreased feeding rates in these fry. The effects of low egg thiamine on both of the behavioral endpoints studied increase the risk of low recruitment rates in Great Lakes lake trout populations.

Oxbow Fish Hatchery Snake River Sockeye Salmon Smolt Program, 2008 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Banks, Duane D.

2009-11-14

This contract proposal is in response to the Federal Columbia River Power System Biological Opinion Implementation Plan/Update Proposed Action (UPA) associated with increasing the number of Snake River sockeye smolts by 150,000. To accomplish this proposal the cooperation and efforts of three government entities has been planned (e.g., Idaho Department of Fish and Game (IDFG), Oregon Department of Fish and Wildlife (ODFW), and the National Marine Fisheries Service (NMFS)). Improvements at the IDFG Eagle Fish Hatchery and NMFS Burley Creek Hatchery will focus on increasing sockeye salmon captive broodstock and egg production. Improvements at the ODFW Oxbow Fish Hatchery willmore » be made to accommodate the incubation, hatching and rearing of 150,000 sockeye salmon smolts for release into Idaho's Sawtooth Valley, Upper Salmon River near IDFG's Sawtooth Fish Hatchery and/or Redfish Lake Creek 1.4 km downstream of Redfish Lake. Modifications to Oxbow Fish Hatchery (ODFW) will include retro-fit existing pond drains so pond cleaning effluent water can be routed to the pollution abatement pond, and modifications to the abatement pond. Also included in this project as an added phase, was the rerouting of the hatchery building effluent water to meet state DEQ guidelines for the use of formalin to treat salmonid eggs. Some additional funding for the described Oxbow Hatchery modifications will come from Mitchell Act Funding. All personnel costs associated with this project will come from Mitchell Act funding. Due to heavy work load issues, being under staffed, and two emergency projects in the spring and summer of 2006, ODFW engineers were not able to complete all plans and get them out for bid in 2006. As a result of these circumstances retro-fitting pond drains and modifications to the abatement pond was carried over into fiscal year 2007-2008. A no cost time extension to the contract was approved by BPA. The format for this report will follow the standard format for Statement of Work Report (SOW), which includes sub-categories Work Element (WE), and within the WE the Milestone Titles.« less

Phytoplankton production systems in a shellfish hatchery: variations of the bacterial load and diversity of vibrios.

PubMed

Dubert, J; Fernández-Pardo, A; Nóvoa, S; Barja, J L; Prado, S

2015-06-01

Outbreaks of disease caused by some Vibrio species represent the main production bottleneck in shellfish hatcheries. Although the phytoplankton used as food is one of the main sources of bacteria, studies of the associated bacterial populations, specifically vibrios, are scarce. The aim of the study was the microbiological monitoring of the microalgae as the first step in assessing the risk disease for bivalve cultures. Two phytoplankton production systems were sampled weekly throughout 1-year period in a bivalve hatchery. Quantitative analysis revealed high levels of marine heterotrophic bacteria in both systems throughout the study. Presumptive vibrios were detected occasionally and at low concentrations. In most of the cases, they belonged to the Splendidus and Harveyi clades. The early detection of vibrios in the microalgae may be the key for a successful bivalve culture. Their abundance and diversity were affected by factors related to the hatchery environment. This work represents the first long study where the presence of vibrios was evaluated rigorously in phytoplankton production systems and provides a suitable microbiological protocol to control and guarantee the quality of the algal cultures to avoid the risk of transferring potential pathogens to shellfish larvae and/or broodstock. © 2015 The Society for Applied Microbiology.

Parental Effect of Long Acclimatization on Thermal Tolerance of Juvenile Sea Cucumber Apostichopus japonicus

PubMed Central

Dong, Yun-wei

2015-01-01

To evaluate the thermal resistance of marine invertebrates to elevated temperatures under scenarios of future climate change, it is crucial to understand parental effect of long acclimatization on thermal tolerance of offspring. To test whether there is parental effect of long acclimatization, adult sea cucumbers (Apostichopus japonicus) from the same broodstock were transplanted southward and acclimatized at high temperature in field mesocosms. Four groups of juvenile sea cucumbers whose parents experienced different durations of high temperature acclimatization were established. Upper thermal limits, oxygen consumption and levels of heat shock protein mRNA of juveniles was determined to compare thermal tolerance of individuals from different groups. Juvenile sea cucumbers whose parents experienced high temperature could acquire high thermal resistance. With the increase of parental exposure duration to high temperature, offspring became less sensitive to high temperature, as indicated by higher upper thermal limits (LT50), less seasonal variations of oxygen consumption, and stable oxygen consumption rates between chronic and acute thermal stress. The relatively high levels of constitutive expression of heat-shock proteins should contribute to the high thermal tolerance. Together, these results indicated that the existence of a parental effect of long acclimatization would increase thermal tolerance of juveniles and change the thermal sensitivity of sea cucumber to future climate change. PMID:26580550

Production and evaluation of YY-male Brook Trout to eradicate nonnative wild brook trout populations

USGS Publications Warehouse

Kennedy, Patrick; Schill, Daniel J.; Meyer, Kevin A.; Campbell, Matthew R.; Vu, Ninh V.; Hansen, Michael J.

2017-01-01

Nonnative Brook Trout Salvelinus fontinalis were introduced throughout western North America in the early 1900s, resulting in widespread self-sustaining populations that are difficult to eradicate and often threaten native salmonid populations. A novel approach for their eradication involves use of YY male (MYY) Brook Trout (created in the hatchery by feminizing XY males and crossing them with normal XY males). If MYY Brook Trout survive after stocking, and reproduce successfully with wild females, in theory this could eventually drive the sex ratio of the wild population to 100% males, at which point the population would not be able to reproduce and would be eradicated. This study represents the first successful development of a FYY and MYY salmonid broodstock, which was produced in four years at relatively low cost. Field trials demonstrated that stocked hatchery MYY Brook Trout survived and produced viable MYY offspring in streams, although reproductive fitness appeared to have been lower than their wild conspecifics. Even if reduced fitness is the norm in both streams and alpine lakes, our population simulations suggest that eradication can be achieved in reasonable time periods under some MYY stocking scenarios, especially when wild Brook Trout are simultaneously suppressed in the population.

Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation.

PubMed

Bar, Ido; Smith, Andre; Bubner, Erin; Yoshizaki, Goro; Takeuchi, Yutaka; Yazawa, Ryosuke; Chen, Ben Nan; Cummins, Scott; Elizur, Abigail

2016-10-01

Germ cell transplantation is an innovative technology for the production of interspecies surrogates, capable of facilitating easier and more economical management of large-bodied broodstock, such as the bluefin tuna. The present study explored the suitability of yellowtail kingfish (Seriola lalandi) as a surrogate host for transplanted southern bluefin tuna (Thunnus maccoyii) spermatogonial cells to produce tuna donor-derived gametes upon sexual maturity. Germ cell populations in testes of donor T. maccoyii males were described using basic histology and the molecular markers vasa and dead-end genes. The peripheral area of the testis was found to contain the highest proportions of dead-end-expressing transplantable Type A spermatogonia. T. maccoyii Type A spermatogonia-enriched preparations were transplanted into the coelomic cavity of 6-10-day-old post-hatch S. lalandi larvae. Fluorescence microscopy and polymerase chain reaction analysis detected the presence of tuna cells in the gonads of the transplanted kingfish fingerlings at 18, 28, 39 and 75 days after transplantation, indicating that the transplanted cells migrated to the genital ridge and had colonised the developing gonad. T. maccoyii germ cell-derived DNA or RNA was not detected at later stages, suggesting that the donor cells were not maintained in the hosts' gonads.

Colorimetric Method of Loop-Mediated Isothermal Amplification with the Pre-Addition of Calcein for Detecting Flavobacterium columnare and its Assessment in Tilapia Farms.

PubMed

Suebsing, Rungkarn; Kampeera, Jantana; Sirithammajak, Sarawut; Withyachumnarnkul, Boonsirm; Turner, Warren; Kiatpathomchai, Wansika

2015-03-01

Flavobacterium columnare, the causative agent of columnaris disease in fish, affects many economically important freshwater fish species. A colorimetric method of loop-mediated isothermal amplification with the pre-addition of calcein (LAMP-calcein) was developed and used to detect the presence of F. columnare in farmed tilapia (Nile Tilapia Oreochromis niloticus and red tilapia [Nile Tilapia × Mozambique Tilapia O. mossambicus]) and rearing water. The detection method, based on a change in color from orange to green, could be performed within 45 min at 63°C. The method was highly specific, as it had no cross-detections with 14 other bacterial species, including other fish pathogens and two Flavobacterium species. The method has a minimum detection limit of 2.2 × 10(2) F. columnare CFU; thus, it is about 10 times more sensitive than conventional PCR. With this method, F. columnare was detected in gonad, gill, and blood samples from apparently healthy tilapia broodstock as well as in samples of fertilized eggs, newly hatched fry, and rearing water. The bacteria isolated from the blood were further characterized biochemically and found to be phenotypically identical to F. columnare. The amplified products from the LAMP-calcein method had 97% homology with the DNA sequence of F. columnare.

Transmission of lymphocystis disease virus to cultured gilthead seabream, Sparus aurata L., larvae.

PubMed

Cano, I; Valverde, E J; Garcia-Rosado, E; Alonso, M C; Lopez-Jimena, B; Ortiz-Delgado, J B; Borrego, J J; Sarasquete, C; Castro, D

2013-06-01

The transmission of lymphocystis disease virus (LCDV) to gilthead seabream, Sparus aurata L., larvae was investigated using fertilized eggs from a farm with previous reports of lymphocystis disease. LCDV genome was detected by PCR-hybridization in blood samples from 17.5% of the asymptomatic gilthead seabream broodstock analysed. Using the same methodology, eggs spawned from these animals were LCDV positive, as well as larvae hatched from them. The presence of infective viral particles was confirmed by cytopathic effects development on SAF-1 cells. Whole-mount in situ hybridization (ISH) and immunohistochemistry (IHC) showed the presence of LCDV in the epidermis of larvae hatched from LCDV-positive eggs. When fertilized eggs were disinfected with iodine, no viral DNA was detected either in eggs (analysed by PCR-hybridization) or in larvae (PCR-hybridization and ISH). These results suggest the vertical transmission of LCDV, the virus being transmitted on the egg surface. Larvae hatched from disinfected eggs remain LCDV negative during the endotrophic phase, as showed by PCR-hybridization, ISH and IHC. After feeding on LCDV-positive rotifers, viral antigens were observed in the digestive tract, which suggests that viral entry could be achieved via the alimentary canal, and that rotifers can act as a vector in LCDV transmission to gilthead seabream larvae. © 2012 Blackwell Publishing Ltd.

Parental Effect of Long Acclimatization on Thermal Tolerance of Juvenile Sea Cucumber Apostichopus japonicus.

PubMed

Wang, Qing-Lin; Yu, Shan-Shan; Dong, Yun-Wei

2015-01-01

To evaluate the thermal resistance of marine invertebrates to elevated temperatures under scenarios of future climate change, it is crucial to understand parental effect of long acclimatization on thermal tolerance of offspring. To test whether there is parental effect of long acclimatization, adult sea cucumbers (Apostichopus japonicus) from the same broodstock were transplanted southward and acclimatized at high temperature in field mesocosms. Four groups of juvenile sea cucumbers whose parents experienced different durations of high temperature acclimatization were established. Upper thermal limits, oxygen consumption and levels of heat shock protein mRNA of juveniles was determined to compare thermal tolerance of individuals from different groups. Juvenile sea cucumbers whose parents experienced high temperature could acquire high thermal resistance. With the increase of parental exposure duration to high temperature, offspring became less sensitive to high temperature, as indicated by higher upper thermal limits (LT50), less seasonal variations of oxygen consumption, and stable oxygen consumption rates between chronic and acute thermal stress. The relatively high levels of constitutive expression of heat-shock proteins should contribute to the high thermal tolerance. Together, these results indicated that the existence of a parental effect of long acclimatization would increase thermal tolerance of juveniles and change the thermal sensitivity of sea cucumber to future climate change.

Aneuploid progeny of the American oyster, Crassostrea virginica, produced by tetraploid × diploid crosses: another example of chromosome instability in polyploid oysters.

PubMed

de Sousa, Joana Teixeira; Allen, Standish K; Baker, Haley; Matt, Joseph L

2016-05-01

The commercial production of triploids, and the creation of tetraploid broodstock to support it, has become an important technique in aquaculture of the eastern oyster, Crassostrea virginica. Tetraploids are produced by cytogenetic manipulation of embryos and have been shown to undergo chromosome loss (to become a mosaic) with unknown consequences for breeding. Our objective was to determine the extent of aneuploidy in triploid progeny produced from both mosaic and non-mosaic tetraploids. Six families of triploids were produced using a single diploid female and crossed with three mosaic and non-mosaic tetraploid male oysters. A second set of crosses was performed with the reciprocals. Chromosome counts of the resultant embryos were tallied at 2-4 cell stage and as 6-hour(h)-old embryos. A significant level of aneuploidy was observed in 6-h-old embryos. For crosses using tetraploid males, aneuploidy ranged from 53% to 77% of observed metaphases, compared to 36% in the diploid control. For crosses using tetraploid females, 51%-71% of metaphases were aneuploidy versus 53% in the diploid control. We conclude that somatic chromosome loss may be a regular feature of early development in triploids, and perhaps polyploid oysters in general. Other aspects of chromosome loss in polyploid oysters are also discussed.

Umatilla Hatchery Monitoring and Evaluation, 1999-2002 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chess, Dale W.; Cameron, William A.; Stonecypher, Jr., R. Wes

2003-12-01

REPORT A: UMATILLA HATCHERY MONITORING AND EVALUATION--This report summarizes monitoring and evaluation studies of salmonids reared at Umatilla Fish Hatchery (UFH) for 1 November, 1999 to 31 October, 2002. Studies at UFH are designed to evaluate rearing of chinook salmon and steelhead in ''Michigan raceways''. Characteristics of Michigan raceways include high fish densities, rapid water turnover, oxygen supplementation, reuse of water, and baffles designed to reduce cleaning. Fish health at UFH and other facilities associated with the Umatilla program are intensively monitored and evaluated along with the overall research project. Further, under the Integrated Hatchery Operations Team guidelines, specific requirementsmore » for fish health monitoring at UFH are mandatory. An experiment designed to evaluate rearing subyearling fall chinook salmon in Michigan and Oregon raceways has been completed. An evaluation of survival of subyearling fall chinook salmon reared at three densities will be completed with final returns in 2005. Two new evaluations were started during this reporting period. The first is an evaluation of spring chinook survival of groups transferred to Imeques acclimation facility in the fall, overwinter-acclimated and released with the standard acclimated production groups in March. The second is an evaluation of subyearling fall chinook survival and straying of a direct-stream released group in the lower Umatilla River and the standard group acclimated at Thornhollow acclimation facility in the upper Umatilla River. An important aspect of the project is evaluation of the spring chinook and summer steelhead fisheries in the upper and lower Umatilla River. REPORT B: Fish Health Monitoring and Evaluation, 2000 Fiscal Year--The results presented in this report are from the ninth year of Fish Health Monitoring and Evaluation in the Umatilla Hatchery program. Broodstock monitoring for hatchery production was conducted on adult returns to the Umatilla River at Three Mile Dam and South Fork Walla Walla adult facilities for salmon; steelhead adults were monitored at Minthorn adult facility. A new addition to this year's report is the effort to bring together an overview of fish health monitoring results including historical and year to date pathogen information. This information is in table form (Appendix Tables A-28, A-29 and A-30). A summary of juvenile disease outbreaks at Umatilla Hatchery is also included (Appendix Table A-31). REPORT C: Fish Health Monitoring and Evaluation, 2001 Fiscal Year--Results from the 2001 annual report cover the 10th year of Fish Health Monitoring in the Umatilla Hatchery program. Efforts were again made to provide up to date fish health and juvenile disease outbreak loss summary tables from the beginning of the Umatilla Hatchery program (Appendix Tables A-27, A-28, A-29 and A-30). Outmigrant Fish Health Monitoring results were included in this report since this was part of the fish health work statement for this report period. The discussion section for the 2001 and 2002 annual reports are combined in the 2002 report due to time constraints and consolidation efforts to complete this report by the end of May 2003.« less

Transcriptome Analysis Based on RNA-Seq in Understanding Pathogenic Mechanisms of Diseases and the Immune System of Fish: A Comprehensive Review

PubMed Central

Sudhagar, Arun; El-Matbouli, Mansour

2018-01-01

In recent years, with the advent of next-generation sequencing along with the development of various bioinformatics tools, RNA sequencing (RNA-Seq)-based transcriptome analysis has become much more affordable in the field of biological research. This technique has even opened up avenues to explore the transcriptome of non-model organisms for which a reference genome is not available. This has made fish health researchers march towards this technology to understand pathogenic processes and immune reactions in fish during the event of infection. Recent studies using this technology have altered and updated the previous understanding of many diseases in fish. RNA-Seq has been employed in the understanding of fish pathogens like bacteria, virus, parasites, and oomycetes. Also, it has been helpful in unraveling the immune mechanisms in fish. Additionally, RNA-Seq technology has made its way for future works, such as genetic linkage mapping, quantitative trait analysis, disease-resistant strain or broodstock selection, and the development of effective vaccines and therapies. Until now, there are no reviews that comprehensively summarize the studies which made use of RNA-Seq to explore the mechanisms of infection of pathogens and the defense strategies of fish hosts. This review aims to summarize the contemporary understanding and findings with regard to infectious pathogens and the immune system of fish that have been achieved through RNA-Seq technology. PMID:29342931

Sex Reversal and Masculinization according to Growth in Longtooth Grouper Epinephelus bruneus

PubMed Central

Oh, Seong-Rip; Kang, Hyeong-Cheol; Lee, Chi-Hoon; Hur, Sang-Woo; Lee, Young-Don

2013-01-01

To understand the sex reversal characteristics in the longtooth grouper (Epinephelus bruneus), this study examined the sex reversal and artificial masculinization of wild caught E. bruneus reared in indoor rearing tank after a 17α-methyltestosterone injection. To domesticate a broodstock, 64 wild caught E. bruneus, between 47.0 to 110.0 cm in total length and from 1.5 to 21.4 kg in body weight, were reared in indoor rearing tank (4.0 to 5.0 m wide, and 2.5 to 3.0 m depth) for four years. Seven specimens showed sex reversal from female to male during indoor rearing condition, whose total length and body weights were from 63.0 to 99.0 cm and from 4.4 to 13.2 kg, respectively. After inducing artificial masculinization in 14 female E. bruneus with a 17α-methyltestosterone (2.0 mg/kg BW) implants for 3 years, spermiation occurred in 9 specimens (total length: 54.0 to 68.0 cm, body weight: 2.3 to 4.3 kg). Among the female to male sex reversals, two specimens returned back to being female, whose body weights were 2.8 kg (initially 2.6 kg) and 2.7 kg (initially 2.3 kg). Therefore, this study suggested that E. bruneus (> 3.0 kg) was more effective in masculinizing by 17α-methyltestosterone implants. PMID:25949124

Effects of ascorbic acid enrichment by immersion of rainbow trout (Oncorhynchus mykiss, Walbaum 1792) eggs and embryos

USGS Publications Warehouse

Falahatkar, B.; Dabrowski, K.; Arslan, M.; Rinchard, J.

2006-01-01

This study was conducted to examine the effects of different forms and concentrations of ascorbic acid (vitamin C), and different enrichment times (24 and 48 h post ovulation) on egg, embryo and alevin ascorbate concentrations and survival of rainbow trout (enrichment was at the ova stage). In experiments 1 and 2, fertilized eggs were immersed in water containing ascorbate at 0 (control), 100, 1000 mg L-1 l-ascorbic acid (AA) and 2000 mg L -1 l-ascorbyl monophosphate (AP). In experiment 3, 0 (control), 500 and 1000 mg L-1 AA neutralized (N) with NaOH, 1000 mg L-1 AA non-neutralized (NN), 1000 and 2000 mg L-1 AP immersions were used. The mean total ascorbic acid (TAA) and dehydroascorbic acid (DHA) concentrations were measured before fertilization, at 3 and 24 h after fertilization, at the eyed stage, and in hatched alevins. We observed significant differences in TAA concentration at different immersion levels at 3 and 24 h after fertilization. Survival decreased significantly depending on the level of vitamin C, pH of the solutions and immersion time. We suggest that when broodstock rainbow trout do not have enough vitamin C in their ovaries, immersion of eggs in 1000 mg L-1 of neutralized AA may be useful. ?? 2006 Blackwell Publishing Ltd.

Genetic diversity and associated pathology of rhabdovirus infections in farmed and wild perch Perca fluviatilis in Ireland.

PubMed

Ruane, N M; Rodger, H D; McCarthy, L J; Swords, D; Dodge, M; Kerr, R C; Henshilwood, K; Stone, D M

2014-12-02

Rhabdovirus infections are an emerging problem for both wild and farmed freshwater fish in Northern Europe. In October 2005, a clinical outbreak with an approximate mortality rate of 40% occurred in a single batch of juvenile perch on a farm in the Republic of Ireland. Clinical signs developed slowly and were consistent with a perch rhabdovirus infection: signs included haemorrhages at the base of the fins and apparent impairment of the central nervous system (manifested as loss of equilibrium and erratic swimming behaviour). Studies suggest that the infected fish originated from a hatchery within the country which relied on wild fish broodstock to supplement the production of perch juveniles. A related rhabdovirus was subsequently isolated from this hatchery. Virus isolation studies have shown that rhabdoviruses were often isolated from wild fish in the vicinity of the hatchery between 1993 and 2005. All isolates were analysed using a generic primer set specific for the L gene of fish vesiculotype viruses. Phylogenetic analysis revealed that all isolates recovered from perch clustered together with the European lake trout rhabdovirus (903/87) of the genus Perhabdovirus. In addition to this, anguillid rhabdovirus was isolated from eel, and the partial L-gene sequence of a previously reported isolate from tench clustered with the pike fry rhabdoviruses, in the genus Sprivivirus.

[Chile's experience with developing abalone (Haliotis spp.) farming: opportunities and challenges].

PubMed

Enríquez, R; Villagrán, R

2008-04-01

Intensive abalone farming--specifically of the red abalone (Haliotis rufescens) and the green (or Japanese) abalone (Haliotis discus hannai)--has expanded rapidly in Chile since the late 1990s, and this article presents an overview of the challenges facing the industry and the factors which favour its development. At present, 100% of Chile's abalone enterprises farm the H. rufescens species, owing to its suitability for full-cycle culture. In the analysis of factors that facilitate the development of abalone farming in Chile, those that stand out include the characteristics of the aquatic ecosystem, existing entrepreneurial and professional skills, decisive government support in co-financing scientific and technological projects, infrastructure and associated services to support these development initiatives and a market where prices have remained stable and demand for abalone products has been steady. The greatest challenges facing intensive abalone farming in Chile are providing a constant supply of macroalgae for abalone feed and developing complementary feed, as well as updating current legislation on intensive abalone farming, strengthening producer associations and establishing health certification. The article discusses examples of the impact that native organisms can have on animals introduced into an aquatic ecosystem and the international transmission of agents such as withering syndrome and sabellid polychaete infestation disease, associated with the movement of abalone seeds and broodstock. The article also emphasises the importance of implementing the recommendations of the World Organisation for Animal Health.

The Pacific bluefin tuna (Thunnus orientalis) dead end gene is suitable as a specific molecular marker of type A spermatogonia.

PubMed

Yazawa, Ryosuke; Takeuchi, Yutaka; Morita, Tetsuro; Ishida, Masashi; Yoshizaki, Goro

2013-10-01

We developed a spermatogonial transplantation technique to produce donor-derived gametes in surrogate fish. Our ultimate aim is to establish surrogate broodstock that can produce bluefin tuna. We previously determined that only type A spermatogonia (ASG) could colonize recipient gonads in salmonids. Therefore, it is necessary to develop a precise molecular marker that can distinguish ASG in order to develop efficient spermatogonial transplantation methods. In this study, the Pacific bluefin tuna (Thunnus orientalis) dead end (BFTdnd) gene was identified as a specific marker for ASG. In situ hybridization and RT-PCR analysis with various types of spermatogenic cell populations captured by laser microdissection revealed that localization of BFTdnd mRNA was restricted to ASG, and not detected in other differentiated spermatogenic cells. In order to determine if BFTdnd can be used as a molecular marker to identify germ cells with high transplantability, transplantation of dissociated testicular cells isolated from juvenile, immature, and mature Pacific bluefin tuna, which have different proportions of dnd-positive ASG, were performed using chub mackerel as the surrogate recipient species. Colonization of transplanted donor germ cells was only successful with testicular cells from immature Pacific Bluefin tuna, which contained higher proportions of dnd-positive ASG than juvenile and mature fish. Thus, BFTdnd is a useful tool for identifying highly transplantable ASG for spermatogonial transplantation. © 2013 Wiley Periodicals, Inc.

Can myxosporean parasites compromise fish and amphibian reproduction?

PubMed

Sitjà-Bobadilla, Ariadna

2009-08-22

Research into fish and amphibian reproduction has increased exponentially in recent years owing to the expansion of the aquaculture industry, the need to recover fishery populations, the impact of endocrine disruptors on the aquatic environment and the global decline of amphibian populations. This review focuses on a group of parasites, the Myxozoa, that affect fish and amphibian reproduction. Lists of the myxosporeans that specifically infect gonads are provided. Most of these are parasitic of freshwater hosts, and most amphibian cases are reported from testes. Sex specificity and sex reversal are discussed in relation to gonadal parasitism. The immune response of the fish to the infection is described, and the contribution of the immunoprivilege of gonads to host invasion is emphasized. The pathological effect of these parasites can be significant, especially in aquacultured broodstocks, on some occasions, leading to parasitic castration. Although myxosporean parasites are currently not very frequent in gonads, their impact could increase in the future owing to the transactions in the global market. Their easy release into the aquatic environment with spawning could make their spreading even more feasible. In the absence of commercial drugs or vaccines to treat and prevent these infections, there is an urgent need to develop specific, rapid and reliable diagnostic tools to control and manage animal movements. In addition, much effort is still to be made on deciphering the life cycle of these organisms, their invasion strategies and their immune evasion mechanisms.

Monitoring of the in-river migration of smolts from two groups of spring chinook salmon, Oncorhynchus tshawytscha (Walbaum), with different profiles of Renibacterium salmoninarum infection

USGS Publications Warehouse

Pascho, R.J.; Elliott, D.G.; Achord, S.

1993-01-01

Broodstock segregation based on the measurement of maternal Renibacterium salmoninarum infection levels by the enzyme-linked immunosorbent assay (ELISA) and the membrane filtration-fluorescent antibody technique (MF-FAT) was previously shown to affect the prevalence and levels of bacterial kidney disease (BKD) in progeny of chinook salmon, Oncorhynchus tshawytscha (Walbaum), during hatchery rearing. Subgroups of fish from that study were marked with passive integrated transponder (PIT) tags, and monitored by PIT-tag detectors during the first 342km of their migration to the Pacific Ocean. Differences in the recovery of tagged fish were significant (P≤ 0·01) at each detection point and became more pronounced as the fish moved downstream. Cumulative recoveries of fish from the low-BKD group and the high-BKD group, respectively, were 31% and 28% after 116km, 44% and 37% after 176km, and 51% and 42% after 342km. There were no apparent differences in the migration timing of the two groups to the first detection point. The data suggested that in-river survival was higher in the progeny group from parents that had low R. salmoninarum infection levels or tested negative for R. salmoninarum (low-BKD group) than in the group female parents with high infection levels (high-BKD group).

Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha)

USGS Publications Warehouse

Hard, J.J.; Elliott, D.G.; Pascho, R.J.; Chase, D.M.; Park, L.K.; Winton, J.R.; Campton, D.E.

2006-01-01

We evaluated genetic variation in ability of Chinook salmon (Oncorhynchus tshawytscha) to resist two bacterial pathogens: Renibacterium salmoninarum, the agent of bacterial kidney disease (BKD), and Listonella anguillarum, an agent of vibriosis. After measuring R. salmoninarum antigen in 499 adults by enzyme-linked immunosorbent assay (ELISA), we mated each of 12 males with high or low antigen levels to two females with low to moderate levels and exposed subsets of their progeny to each pathogen separately. We found no correlation between R. salmoninarum antigen level in parents and survival of their progeny following pathogen exposure. We estimated high heritability for resistance to R. salmoninarum (survival h2 = 0.890 ?? 0.256 (mean ?? standard error)) independent of parental antigen level, but low heritability for resistance to L. anguillarum (h2 = 0.128 ?? 0.078). The genetic correlation between these survivals (rA = -0.204 ?? 0.309) was near zero. The genetic and phenotypic correlations between survival and antigen levels among surviving progeny exposed to R. salmoninarum were both negative (rA = -0.716 ?? 0.140; rP = -0.378 ?? 0.041), indicating that variation in antigen level is linked to survival. These results suggest that selective culling of female broodstock with high antigen titers, which is effective in controlling BKD in salmon hatcheries, will not affect resistance of their progeny. ?? 2006 NRC.

Morphometric Relationship, Growth, and Condition Factor of Flyingfish, Hirundicththys oxycephalus during spawning season

NASA Astrophysics Data System (ADS)

Tuapetel, Friesland; Nessa, Natsir; Alam Ali, Syamsu; Sudirman; Hutubessy, B. G.; Mosse, J. W.

2017-10-01

Flyingfish, Hirundichthys oxycephalus together with its congener species has become a major artisanal fishery resource from the Seram Sea, an area between Seram and Papua Island. However, biological information about this species has been poorly documented. Our current study focused on the growth and morphometric relationships of spawning cohorts during spawning season (June to October 2013). Flyingfish which were trapped in bale-bale, an egg aggregation device for flying fish, were collected offshore of the Southeastern Seram Sea. Total samples of 1693 with Fork Length (FL) ranged from 156.15mm to 245.52mm and total weight from 53.15gr to 115.45gr. Von Bertalanffy growth functions (L∞) for-pooled sexes were FL = 251mm [1-e (1.82 (t+0.060)], K=1.82 and t0 = 0.060. Morphometrically, significant differences were observed for all individuals between sexes (F=14.20, P=0.0002), sampling locations (F=88.48 P<0.0001) and sampling period (F=138.84, P<0.0001). Condition factor of the fish generally declined in July-August. During spawning season, this fish tended to form single spawning cohort. The results of this study provide a significant understanding of the life history of this valuable fish inhabiting this particular region that rarely receives scientific investigation. For management purposes, harvesting eggs including broodstocks will lead to critical population depletion.

First identification of Francisella noatunensis subsp. orientalis causing mortality in Mexican tilapia Oreochromis spp.

PubMed

Ortega, Cesar; Mancera, Gerardo; Enríquez, Ricardo; Vargas, Augusto; Martínez, Simón; Fajardo, Raúl; Avendaño-Herrera, Ruben; Navarrete, María José; Romero, Alex

2016-08-09

Francisellosis, an emerging disease in tilapia Oreochromis spp., is caused by the facultative, intracellular bacterium Francisella noatunensis subsp. orientalis, which is present in various countries where tilapia farming is commercially important. We confirmed the presence of francisellosis in Mexican tilapia cultures in association with an outbreak during the second semester of 2012. Broodstock fish presented a mortality rate of approximately 40%, and disease was characterized by histologically classified granulomas, or whitish nodules, in different organs, mainly the spleen and kidney. Through DNA obtained from infected tissue and pure cultures in a cysteine heart medium supplemented with hemoglobin, F. noatunensis subsp. orientalis was initially confirmed through the amplification and analysis of the 16S rRNA gene and the internal transcribed spacer region. Phylogenetic analysis of these genes demonstrated close similarity with previously reported F. noatunensis subsp. orientalis sequences obtained from infected tilapia from various countries. The identification of this subspecies as the causative agent of the outbreak was confirmed using the iglC gene as a target sequence, which showed 99.5% identity to 2 F. noatunensis subsp. orientalis strains (Ethime-1 and Toba04). These findings represent the first documented occurrence of francisellosis in Mexican tilapia cultures, which highlights the importance of establishing preventative measures to minimize the spread of this disease within the Mexican aquaculture industry.

Use of electronarcosis to immobilize juvenile and adult northern pike

USGS Publications Warehouse

Walker, M.K.; Yanke, E.A.; Gingerich, W.H.

1994-01-01

Electronarcosis, the immobilization of a fish after an electric current has been applied and discontinued, is a potential alternative to chemical anesthetics. Successful narcosis was defined as the immobilization of a fish for 1-15 min without causing physical damage. In the laboratory, AC successfully narcotized juvenile (13-19-cm standard length, SL) northern pike (Esox lucius) at selected voltages; however, AC voltages that produced narcosis or resulted in physical damage were variable and unpredictable. In contrast, 60-90-V pulsed DC (PDC) for 10-60 s successfully narcotized juvenile pike without inducing physical damage. Duration of narcosis was directly related to voltage and inversely related to fish length. In the hatchery, sexually mature northern pike (45-97 cm SL), collected from the Mississippi River, were successfully narcotized by 60-V PDC for 10 s. Duration of narcosis was unrelated to fish length or sex, and averaged 58 plus or minus 7 s (mean plus or minus SE). This allowed sufficient time to collect eggs or milt. All fish were swimming upright within 3 min after treatment, and no mortalities were observed over the next 24 h. Survival of eggs from fertilization to eye- up did not significantly differ between eggs collected from electronarcotized adults and adults anesthetized with MS-222 (tricaine methanesulfonate). Electronarcosis represents a possible alternative to chemical anesthetics for immobilizing northern pike broodstock without an apparent impact on egg survival.

[Reproductive biology and artificial propagation of Acipenser sinensis below Gezhouba Dam].

PubMed

Liu, Jian-yi; Wei, Qi-wei; Chen, Xi-hua; Yang, De-guo; Du, Hao; Zhu, Yong-jiu

2007-06-01

A total of 36 females and 21 males of Chinese sturgeon Acipenser sinensis were caught in 1998-2004 excluding 2002 to study the characteristics of their reproductive biology and the effect of their artificial propagation. The results showed that the body length (BL), body mass (BM) and age of the females were 240-320 cm, 140-432 kg, and 15-30 years, and those of the males were 153-284 cm, 70-244 kg and 12-26 years, respectively. The inducing rate was 93.1% for females and 100% for males, and the ova had 7 different colors. The absolute fecundity was 200,000-590,000 eggs, with an average of 358,000 eggs, and the relative fecundity to BM was 820-3,020 eggs per kg, with an average of 1,590 eggs per kg. The sperm had 4 different colors. The absolute sperm quantity obtained from one male was 1,000-5,952 ml, with an average of 2,597.8 ml, and the relative sperm quantity to BM was 1.25-31.24 ml . kg(-1), with an average of 13.3 ml . kg(-1). During the study period, the average fertilization rate in artificial propagation was 63.7%, and the hatching rate was 48.1%, with 4,762,000 fry obtained. Compared with the data in 1976, the natural reproductive capacity of the Chinese sturgeon broodstocks declined greatly.

Comparison of methods to improve induction of spermiation in wild-caught carp (Cyprinus carpio carpio), a threatened species from the Caspian Sea basin.

PubMed

Vazirzadeh, Arya; Farhadi, Ahmad; Naseri, Mahmood; Jeffs, Andrew

2016-07-01

Wild carp (Cyprinus carpio carpio) forms the basis of an important fishery in the Southern Caspian Sea Basin which is increasingly underpinned by the release of cultured juveniles. A significant bottleneck to hatchery-rearing of juveniles is the spermiation of male broodstock. Therefore, four approaches to improving spermiation were investigated. The effectiveness of two delivery methods for the sustained release of salmon gonadotropin releasing hormone analogue (sGnRHa; i.e., via intramuscular cholesterol pellet vs emulsion injection) on the spermiation success and duration, sperm quality and quantity over 14days in wild-caught carp were compared to single injection of sGnRHa with Pimozide(®) (Linpe method) or carp pituitary extract (CPE). The consequence of the spermiation treatments on resulting embryonic quality was evaluated for subsequent fertilization and hatching success from wild male carp (mean weight±S.D. 1021±112g). All hormonal treatments, except for Linpe method, led to 100% spermiation of treated fish compared to only 25% in the control with no hormone intervention. The duration of spermiation, as well as the various quantitative variables of the sperm and the mean total sperm production were all generally improved with the sustained hormone delivery compared with the acute treatments. The GnRHa-FIA was the most effective method for improving spermiation. Copyright © 2016. Published by Elsevier B.V.

22 CFR 146.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Education programs or activities. 146.400... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 146.400 Education programs or activities...

49 CFR 25.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 1 2011-10-01 2011-10-01 false Education programs or activities. 25.400 Section... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 25.400 Education programs or activities...

22 CFR 146.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Education programs or activities. 146.400... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 146.400 Education programs or activities...

49 CFR 25.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Education programs or activities. 25.400 Section... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 25.400 Education programs or activities...

28 CFR 54.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Education programs or activities. 54.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 54.400 Education programs or activities...

22 CFR 229.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Education programs or activities. 229.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 229.400 Education programs or activities...

22 CFR 229.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Education programs or activities. 229.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 229.400 Education programs or activities...

10 CFR 1042.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Education programs or activities. 1042.400 Section 1042... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1042.400 Education programs or activities. (a...

12 CFR 268.707 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Programs and Activities Because of Physical or Mental Disability § 268.707 Program accessibility: Existing facilities. (a) General. The Board shall operate each program or activity so that the program or activity... can demonstrate would result in a fundamental alteration in the nature of a program or activity or in...

12 CFR 268.707 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Programs and Activities Because of Physical or Mental Disability § 268.707 Program accessibility: Existing facilities. (a) General. The Board shall operate each program or activity so that the program or activity... can demonstrate would result in a fundamental alteration in the nature of a program or activity or in...

41 CFR 101-4.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 41 Public Contracts and Property Management 2 2011-07-01 2007-07-01 true Education programs or... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 101-4.400 Education programs or activities. (a...

41 CFR 101-4.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Education programs or... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 101-4.400 Education programs or activities. (a...

Salmonid Gamete Preservation in the Snake River Basin, 2001 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Armstrong, Robyn; Kucera, Paul

2002-06-01

Steelhead (Oncorhynchus mykiss) and chinook salmon (Oncorhynchus tshawytscha) populations in the Northwest are decreasing. Genetic diversity is being lost at an alarming rate. Along with reduced population and genetic variability, the loss of biodiversity means a diminished environmental adaptability. The Nez Perce Tribe (Tribe) strives to ensure availability of genetic samples of the existing male salmonid population by establishing and maintaining a germplasm repository. The sampling strategy, initiated in 1992, has been to collect and preserve male salmon and steelhead genetic diversity across the geographic landscape by sampling within the major river subbasins in the Snake River basin, assuming amore » metapopulation structure existed historically. Gamete cryopreservation conserves genetic diversity in a germplasm repository, but is not a recovery action for listed fish species. The Tribe was funded in 2001 by the Bonneville Power Administration (BPA) and the U.S. Fish and Wildlife Service Lower Snake River Compensation Plan (LSRCP) to coordinate gene banking of male gametes from Endangered Species Act (ESA) listed steelhead and spring and summer chinook salmon in the Snake River basin. In 2001, a total of 398 viable chinook salmon semen samples from the Lostine River, Catherine Creek, upper Grande Ronde River, Lookingglass Hatchery (Imnaha River stock), Lake Creek, the South Fork Salmon River weir, Johnson Creek, Big Creek, Capehorn Creek, Marsh Creek, Pahsimeroi Hatchery, and Sawtooth Hatchery (upper Salmon River stock) were cryopreserved. Also, 295 samples of male steelhead gametes from Dworshak Hatchery, Fish Creek, Grande Ronde River, Little Sheep Creek, Pahsimeroi Hatchery and Oxbow Hatchery were also cryopreserved. The Grande Ronde chinook salmon captive broodstock program stores 680 cryopreserved samples at the University of Idaho as a long-term archive, half of the total samples. A total of 3,206 cryopreserved samples from Snake River basin steelhead and spring and summer chinook salmon, from 1992 through 2001, are stored in two independent locations at the University of Idaho (UI) and Washington State University (WSU). Two large freezer tanks are located at each university. Recommendations for future gene banking efforts include the need for establishment of a regional genome resource bank, an emphasis on cryopreserving wild unmarked fish, continued fertility trials, and genetic analysis on all fish represented in the germplasm repository.« less

40 CFR 5.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Education programs or activities. 5.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 5.400 Education programs or...

14 CFR 1253.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Education programs or activities. 1253.400... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1253.400 Education programs...

43 CFR 41.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false Education programs or activities. 41.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 41.400 Education programs or...

45 CFR 86.31 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Education programs or activities. 86.31 Section 86... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs or...

6 CFR 17.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 6 Domestic Security 1 2011-01-01 2011-01-01 false Education programs or activities. 17.400 Section... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 17.400 Education programs or...

31 CFR 28.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Education programs or activities. 28... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 28.400 Education programs or...

45 CFR 1175.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL ENDOWMENT FOR THE... program or activity so that the program or activity, when viewed in its entirety, is readily accessible to... of a program or activity or in undue financial and administrative burdens. In those circumstances...

31 CFR 17.150 - Program accessibility; Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

...'s facilities that limit the physical accessibility of its programs or activities to individuals with... ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE... operate each program or activity so that the program or activity, when viewed in its entirety, is readily...

6 CFR 15.50 - Program accessibility; existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF DISABILITY IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE... Department shall operate each program or activity so that the program or activity, when viewed in its... result in a fundamental alteration in the nature of a program or activity or in undue financial and...

38 CFR 15.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... AFFAIRS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY.... The agency shall operate each program or activity so that the program or activity, when viewed in its... fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens...

22 CFR 219.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY INTERNATIONAL DEVELOPMENT.... (a) General. The agency shall operate each program or activity so that the program or activity, when... result in a fundamental alteration in the nature of a program or activity or in undue financial and...

16 CFR 6.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... OF PRACTICE ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES.... The Commission shall operate each program or activity so that the program or activity, when viewed in... demonstrate would result in a fundamental alteration in the nature of a program or activity or in undue...

24 CFR 9.150 - Program accessibility: existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... ACTIVITIES CONDUCTED BY THE DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT § 9.150 Program accessibility... shall operate each program or activity so that the program or activity, when viewed in its entirety, is... nature of a program or activity or in undue financial and administrative burdens. In those circumstances...

36 CFR 812.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... HISTORIC PRESERVATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES... facilities. (a) General. The agency shall operate each program or activity so that the program or activity... fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens...

12 CFR 606.650 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE FARM CREDIT... program or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

18 CFR 1313.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... AUTHORITY ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY... agency shall operate each program or activity so that the program or activity, when viewed in its... the nature of a program or activity or in undue financial and administrative burdens. In those...

29 CFR 2205.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY... facilities. (a) General. The agency shall operate each program or activity so that the program or activity... fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens...

45 CFR 606.51 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY... Foundation shall operate each program or activity so that the program or activity, when viewed in its... result in a fundamental alteration in the nature of a program or activity or in undue financial and...

29 CFR 100.550 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Enforcement of Nondiscrimination on the Basis of Handicap in Programs or Activities Conducted by the National... shall operate each program or activity so that the program or activity, when viewed in its entirety, is... the nature of a program or activity or in undue financial and administrative burdens. In those...

40 CFR 12.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE ENVIRONMENTAL PROTECTION... program or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

45 CFR 1214.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... ACTIVITIES CONDUCTED BY ACTION § 1214.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is... fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens...

29 CFR 2706.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY... facilities. (a) General. The agency shall operate each program or activity so that the program or activity... fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens...

22 CFR 1701.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE UNITED STATES INSTITUTE OF... program or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

36 CFR 909.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... DEVELOPMENT CORPORATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES... facilities. (a) General. The agency shall operate each program or activity so that the program or activity... fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens...

5 CFR 2416.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE FEDERAL... shall operate each program or activity so that the program or activity, when viewed in its entirety, is... fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens...

17 CFR 149.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY...) General. The agency shall operate each program or activity so that the program or activity, when viewed in... the nature of a program or activity or in undue financial and administrative burdens. In those...

45 CFR 1181.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE INSTITUTE OF MUSEUM AND... operate each program or activity so that the program or activity, when viewed in its entirety, is readily... the nature of a program or activity or in undue financial and administrative burdens. In those...

14 CFR 1253.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Education programs or activities. 1253.400... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1253.400 Education programs...

45 CFR 618.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Education programs or activities. 618.400 Section... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 618.400 Education programs or...

31 CFR 28.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Education programs or activities. 28... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 28.400 Education programs or...

6 CFR 17.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 6 Domestic Security 1 2010-01-01 2010-01-01 false Education programs or activities. 17.400 Section... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 17.400 Education programs or...

Risk factors perceived predictive of ISA spread in Chile: applications to decision support.

PubMed

Gustafson, L; Antognoli, M; Lara Fica, M; Ibarra, R; Mancilla, J; Sandoval Del Valle, O; Enriquez Sais, R; Perez, A; Aguilar, D; Madrid, E; Bustos, P; Clement, A; Godoy, M G; Johnson, C; Remmenga, M

2014-11-01

Aquaculture is anticipated to be a critical element in future solutions to global food shortage. However, diseases can impede industry efficiency and sustainability. Consequently, diseases can and have led to dramatic re-structuring in industry or regulatory practices. The emergence of infectious salmon anemia (ISA) in Chile is one such example. As in other countries, many mitigations were instituted universally, and many incurred considerable costs as they introduced a new layer of coordination of farming activities of marine sites within common geographic areas (termed 'neighborhoods' or 'barrios'). The aggregate response led to a strong reduction in ISA incidence and impact. However, the relative value of individual mitigations is less clear, especially where response policies were universally applied and retrospective analyses are missing 'controls' (i.e., areas where a mitigation was not applied). Further, re-focusing policies around disease prevention following resolution of an outbreak is important to renew sustainable production; though, again, field data to guide this shift in purpose are often lacking. Expert panels can offer timely decision support in the absence of empirical data. We convened a panel of fish health experts to weight risk factors predictive of ISA virus (ISAV) introduction or spread between Atlantic salmon barrios in Chile. Barrios, rather than sites, were the unit of interest because many of the new mitigations operate at this level and few available studies examine their efficacy. Panelists identified barrio processing plant biosecurity, fallowing strategies, adult live fish transfers, fish and site density, smolt quality, hydrographic connection with other neighborhoods, presence of sea lice (Caligus rogercresseyi), and harvest vessel biosecurity as factors with the greatest predictive strength for ISAV virulent genotype ('HPR-deleted') occurrence. Fewer factors were considered predictive of ISAV HPR0 genotype ('HPR0') occurrence, with greatest strengths assigned to fish and site density, adult live fish transfers, and smolt facility HPR0 status. Field validation based on ISAV and risk factor occurrence after panel completion generally supports expert estimates, and highlights a few factors (e.g., broodstock HPR0 status) less conclusive in the original study. Results inform legislation, industry best management practices and surveillance design. Published by Elsevier B.V.

76 FR 65428 - Classification and Program Review

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-21

... review will be an abbreviated program review meant to focus on an inmate's programming activities. This... during his/her incarceration. The plan will ordinarily include work and programming activities to help... an inmate's programming activities. This shortened version of the more thorough program review will...

15 CFR 8a.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs or...

45 CFR 85.42 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF HEALTH AND HUMAN... program or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

15 CFR 8a.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs or...

School-Age Ideas and Activities for After School Programs.

ERIC Educational Resources Information Center

Haas-Foletta, Karen; Cogley, Michele

This guide describes activities for school-age children in after-school day care programs. These activities may also be used in other settings. An introductory section discusses program philosophy, room arrangement, multicultural curriculum, program scheduling, summer programs and holiday care, field trips and special programs, age grouping,…

US medical specialty global health training and the global burden of disease

PubMed Central

Kerry, Vanessa B.; Walensky, Rochelle P.; Tsai, Alexander C.; Bergmark, Regan W.; Bergmark, Brian A.; Rouse, Chaturia; Bangsberg, David R.

2013-01-01

Background Rapid growth in global health activity among US medical specialty education programs has lead to heterogeneity in types of activities and global health training models. The breadth and scope of this activity is not well chronicled. Methods Using a standardized search protocol, we examined the characteristics of US medical residency global health programs by number of programs, clinical specialty, nature of activity (elective, research, extended curriculum based field training), and geographic location across seven different clinical medical residency education specialties. We tabulated programmatic activity by clinical discipline, region and country. We calculated the Spearman's rank correlation coefficient to estimate the association between programmatic activity and country–level disease burden. Results Of the 1856 programs assessed between January and June 2011, there were 380 global health residency training programs (20%) working in 141 countries. 529 individual programmatic activities (elective–based rotations, research programs, extended curriculum–based field training, or other) occurred at 1337 specific sites. The majority of the activities consisted of elective–based rotations. At the country level, disease burden had a statistically significant association with programmatic activity (Spearman's ρ = 0.17) but only explained 3% of the total variation between countries. Conclusions There were a substantial number of US medical specialty global health programs, but a relative paucity of surgical and mental health programs. Elective–based programs were more common than programs that offer longitudinal experiences. Despite heterogeneity, there was a small but statistically significant association between program location and the global burden of disease. Areas for further study include the degree to which US–based programs develop partnerships with their program sites, the significance of this activity for training, and number and breadth of programs in medical specialty global health education in other countries around the world. PMID:24363924

32 CFR 196.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false Education programs or activities. 196.400... (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities...

32 CFR 196.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Education programs or activities. 196.400... (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities...

34 CFR 403.71 - In what additional ways may funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2010 CFR

2010-07-01

... State Programs and State Leadership Activities? 403.71 Section 403.71 Education Regulations of the... Secretary Assist Under the Basic Programs? State Programs and State Leadership Activities § 403.71 In what additional ways may funds be used under the State Programs and State Leadership Activities? In addition to...

34 CFR 403.71 - In what additional ways may funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2011 CFR

2011-07-01

... State Programs and State Leadership Activities? 403.71 Section 403.71 Education Regulations of the... Secretary Assist Under the Basic Programs? State Programs and State Leadership Activities § 403.71 In what additional ways may funds be used under the State Programs and State Leadership Activities? In addition to...

34 CFR 403.71 - In what additional ways may funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2013 CFR

2013-07-01

... State Programs and State Leadership Activities? 403.71 Section 403.71 Education Regulations of the... Secretary Assist Under the Basic Programs? State Programs and State Leadership Activities § 403.71 In what additional ways may funds be used under the State Programs and State Leadership Activities? In addition to...

34 CFR 403.71 - In what additional ways may funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2012 CFR

2012-07-01

... State Programs and State Leadership Activities? 403.71 Section 403.71 Education Regulations of the... Secretary Assist Under the Basic Programs? State Programs and State Leadership Activities § 403.71 In what additional ways may funds be used under the State Programs and State Leadership Activities? In addition to...

34 CFR 403.71 - In what additional ways may funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2014 CFR

2014-07-01

... State Programs and State Leadership Activities? 403.71 Section 403.71 Education Regulations of the... Secretary Assist Under the Basic Programs? State Programs and State Leadership Activities § 403.71 In what additional ways may funds be used under the State Programs and State Leadership Activities? In addition to...

13 CFR 113.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Education programs or activities... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Education Programs Or Activities Prohibited § 113...

45 CFR 2555.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 4 2011-10-01 2011-10-01 false Education programs or activities. 2555.400 Section... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities...

24 CFR 3.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Education programs or activities. 3... Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities...

28 CFR 54.400 - Education programs or activities.

Code of Federal Regulations, 2013 CFR

2013-07-01

... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 54.400 Education programs or activities... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected to...

13 CFR 113.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Education programs or activities... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Education Programs Or Activities Prohibited § 113...

45 CFR 2555.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false Education programs or activities. 2555.400 Section... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities...

24 CFR 3.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Education programs or activities. 3... Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities...

Can Myxosporean parasites compromise fish and amphibian reproduction?

PubMed Central

Sitjà-Bobadilla, Ariadna

2009-01-01

Research into fish and amphibian reproduction has increased exponentially in recent years owing to the expansion of the aquaculture industry, the need to recover fishery populations, the impact of endocrine disruptors on the aquatic environment and the global decline of amphibian populations. This review focuses on a group of parasites, the Myxozoa, that affect fish and amphibian reproduction. Lists of the myxosporeans that specifically infect gonads are provided. Most of these are parasitic of freshwater hosts, and most amphibian cases are reported from testes. Sex specificity and sex reversal are discussed in relation to gonadal parasitism. The immune response of the fish to the infection is described, and the contribution of the immunoprivilege of gonads to host invasion is emphasized. The pathological effect of these parasites can be significant, especially in aquacultured broodstocks, on some occasions, leading to parasitic castration. Although myxosporean parasites are currently not very frequent in gonads, their impact could increase in the future owing to the transactions in the global market. Their easy release into the aquatic environment with spawning could make their spreading even more feasible. In the absence of commercial drugs or vaccines to treat and prevent these infections, there is an urgent need to develop specific, rapid and reliable diagnostic tools to control and manage animal movements. In addition, much effort is still to be made on deciphering the life cycle of these organisms, their invasion strategies and their immune evasion mechanisms. PMID:19474043

Genomics and introgression: discovery and mapping of thousands of species-diagnostic SNPs using RAD sequencing

USGS Publications Warehouse

Hand, Brian K.; Hether, Tyler D; Kovach, Ryan P.; Muhlfeld, Clint C.; Amish, Stephen J.; Boyer, Matthew C.; O’Rourke, Sean M.; Miller, Michael R.; Lowe, Winsor H.; Hohenlohe, Paul A.; Luikart, Gordon

2015-01-01

Invasive hybridization and introgression pose a serious threat to the persistence of many native species. Understanding the effects of hybridization on native populations (e.g., fitness consequences) requires numerous species-diagnostic loci distributed genome-wide. Here we used RAD sequencing to discover thousands of single-nucleotide polymorphisms (SNPs) that are diagnostic between rainbow trout (RBT, Oncorhynchus mykiss), the world’s most widely introduced fish, and native westslope cutthroat trout (WCT, O. clarkii lewisi) in the northern Rocky Mountains, USA. We advanced previous work that identified 4,914 species-diagnostic loci by using longer sequence reads (100 bp vs. 60 bp) and a larger set of individuals (n = 84). We sequenced RAD libraries for individuals from diverse sampling sources, including native populations of WCT and hatchery broodstocks of WCT and RBT. We also took advantage of a newly released reference genome assembly for RBT to align our RAD loci. In total, we discovered 16,788 putatively diagnostic SNPs, 10,267 of which we mapped to anchored chromosome locations on the RBT genome. A small portion of previously discovered putative diagnostic loci (325 of 4,914) were no longer diagnostic (i.e., fixed between species) based on our wider survey of non-hybridized RBT and WCT individuals. Our study suggests that RAD loci mapped to a draft genome assembly could provide the marker density required to identify genes and chromosomal regions influencing selection in admixed populations of conservation concern and evolutionary interest.

Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis.

PubMed

Nam, Y K; Noh, J K; Cho, Y S; Cho, H J; Cho, K N; Kim, C G; Kim, D S

2001-08-01

Transgenic mud loaches (Misgurnus mizolepis), in which the entire transgene originated from the same species, have been generated by microinjecting the mud loach growth hormone (mlGH) gene fused to the mud loach beta-actin promoter. Out of 4,100 eggs injected, 7.5% fish derived from the injected eggs showed dramatically accelerated growth, with a maximum of 35-fold faster growth than their non-transgenic siblings. Many fast-growing transgenic individuals showed extraordinary gigantism: their body weight and total length (largest fish attained to 413 g and 41.5 cm) were larger and longer than even those of 12-year-old normal broodstock (maximum size reached to 89 g and 28 cm). Of 46 transgenic founders tested, 30 individuals transmitted the transgene to next generation with a wide range of germ-line transmission frequencies ranging from 2% to 33%. The growth performance of the subsequent generation (F1) was also dramatically accelerated up to 35-fold, although the levels of enhanced growth were variable among transgenic lines. Three transgenic germ-lines up to F4 were established, showing the expected Mendelian inheritance of the transgene. Expression of GH mRNA in many tissues was detected by RT-PCR analyses. The time required to attain marketable size (10 g) in these transgenic lines was only 30-50 days after fertilization, while at least 6 months in non-transgenic fish. Besides growth enhancement, significantly improved feed-conversion efficiency up to 1.9-fold was also observed.

Genetic variability comparison of cultured Israeli carp (Cyprinus carpio) from Korea using microsatellites.

PubMed

Kim, Jung Eun; Goo, In Bon; Hwang, Ju-Ae; Kim, Hyeong Su; Choi, Hye-Sung; Lee, Jeong-Ho

2018-06-01

In aquaculture, cultured fish often undergo continuous cross-fertilization without any inflow of new broodstock. This lowers genetic diversity, leading to increased disease rates and decreased survival rates. To improve the mass production and easy culture of Israeli carp, it is essential to investigate the population structure and genetic diversity of these fish. However, such a survey has not yet been performed on Korean Israeli carp. In this study, we used seven microsatellite markers to analyze the genetic diversity and association of cultured Israeli carp from Korea and China. The average numbers of alleles per locus (N A ) for two Korean (KorA and KorB) and two Chinese (ChA and ChB) populations were as follows: KorA (10.42), KorB (14.43), ChA (20.57) and ChB (20.71). The expected heterozygosity (H e ) ranged from 0.672 to 0.897 and from 0.827 to 0.938 in the Korean sample and Chinese sample respectively. The genetic diversity of the Korean Israeli carp was about half that of the Chinese carp. The diversity of the Korean Israeli carp was very low, suggesting that the immunity of this population could be weak, and that diversity-recovery studies are urgently needed. Therefore, our results may therefore form the foundation for future research efforts towards genetic monitoring and selective breeding, continuous research needs to be conducted in order to recover the genetic diversity of the Korean Israeli carp.

Experimental infection of six North American fish species with the North Carolina strain of spring Viremia of Carp Virus

USGS Publications Warehouse

Emmenegger, Eveline J.; Sanders, George E.; Conway, Carla M.; Binkowski, Fred P.; Winton, James R.; Kurath, Gael

2016-01-01

Three salmonid species, rainbow and steelhead trout (Oncorhynchus mykiss), Chinook salmon (O. tshawytscha), and sockeye salmon (O. nerka), were challenged by immersion or injection with the North Carolina SVCV isolate. Two cyprinid species, koi and fathead minnow (Pimephales promelas) and one percid species, yellow perch (Perca flavescens) were also challenged. Koi were highly susceptible to SVCV up to 11 months of age and fathead minnows had chronic disease expression with moderate mortality (29%). SVCV also induced moderate mortalities (33%) in yellow perch fry. Virus challenged salmonid fish had cumulative percent mortalities ranging from 0 to 100%, with sockeye salmon fry being the most vulnerable. A sub-sample of mortalities and survivors were screened for virus by plaque assay and reverse transcription polymerase chain reaction. In general, all mortalities tested positive for SVCV with high viral titers while survivors had variable persistence of SVCV with overall lower virus titers. Our SVCV challenges of multiple North American fish species suggested that host age is a key factor in determining disease outcome. Other factors, such as fish broodstock, virus strain, water temperature, and rearing conditions in association with the intrinsic level of species susceptibility may also impact infection dynamics. This is the first report of SVCV infecting a species (yellow perch) in the family Percidae and that sockeye salmon fry can suffer similarly high mortalities as the primary SVCV host species.

Tracing the first steps of American sturgeon pioneers in Europe

USGS Publications Warehouse

Ludwig, A.; Arndt, U.; Lippold, S.; Benecke, N.; Debus, L.; King, T.L.; Matsumura, S.

2008-01-01

Background. A Baltic population of Atlantic sturgeon was founded ???1,200 years ago by migrants from North America, but after centuries of persistence, the population was extirpated in the 1960s, mainly as a result of over-harvest and habitat alterations. As there are four genetically distinct groups of Atlantic sturgeon inhabiting North American rivers today, we investigated the genetic provenance of the historic Baltic population by ancient DNA analyses using mitochondrial and nuclear markers. Results. The phylogeographic signal obtained from multilocus microsatellite DNA genotypes and mitochondrial DNA control region haplotypes, when compared to existing baseline datasets from extant populations, allowed for the identification of the region-of-origin of the North American Atlantic sturgeon founders. Moreover, statistical and simulation analyses of the multilocus genotypes allowed for the calculation of the effective number of individuals that originally founded the European population of Atlantic sturgeon. Our findings suggest that the Baltic population of A. oxyrinchus descended from a relatively small number of founders originating from the northern extent of the species' range in North America. Conclusion. These results demonstrate that the most northerly distributed North American A. oxyrinchus colonized the Baltic Sea ???1,200 years ago, suggesting that Canadian specimens should be the primary source of broodstock used for restoration in Baltic rivers. This study illustrates the great potential of patterns obtained from ancient DNA to identify population-of-origin to investigate historic genotype structure of extinct populations. ?? 2008 Ludwig et al; licensee BioMed Central Ltd.

Transgenerational marking of freshwater fishes with enriched stable isotopes: a tool for fisheries management and research.

PubMed

Munro, A R; Gillanders, B M; Thurstan, S; Crook, D A; Sanger, A C

2009-08-01

A promising new method of marking larval freshwater fishes with enriched stable isotopes by means of injecting the maternal parent with the marking agent was investigated. The (138)Ba:(137)Ba ratios in the otoliths of larval golden perch Macquaria ambigua were compared to determine the effect of injecting female broodstock with different dosages of enriched (137)Ba at various times before spawning. There was 100% mark success in the progeny of fish injected with 20 microg g(-1) of enriched (137)Ba 24 h before inducing spawning with hormones and 40 microg g(-1) administered at the same time as inducement of spawning. Injection of 40 microg g(-1) enriched (137)Ba 21 days before spawning resulted in only 81% mark success and suggests rapid elimination of barium in M. ambigua. Injection with enriched (137)Ba did not significantly affect the fertilization rate, number of fertilized eggs or hatching rate compared with long-term hatchery records. These results suggest that transgenerational marking is an effective and affordable means of mass-marking larval fishes. Thousands of larval fishes can be permanently marked with a unique artificial isotopic mark via a single injection into the maternal parent, thus avoiding the handling of individual fishes or having to deal with chemical baths. Because no single mark or tagging method is suitable for all situations, transgenerational marking with enriched stable isotopes provides another method for researchers and managers to discriminate both hatchery-reared and wild fishes.

Perspectives: Gene Expression in Fisheries Management

USGS Publications Warehouse

Nielsen, Jennifer L.; Pavey, Scott A.

2010-01-01

Functional genes and gene expression have been connected to physiological traits linked to effective production and broodstock selection in aquaculture, selective implications of commercial fish harvest, and adaptive changes reflected in non-commercial fish populations subject to human disturbance and climate change. Gene mapping using single nucleotide polymorphisms (SNPs) to identify functional genes, gene expression (analogue microarrays and real-time PCR), and digital sequencing technologies looking at RNA transcripts present new concepts and opportunities in support of effective and sustainable fisheries. Genomic tools have been rapidly growing in aquaculture research addressing aspects of fish health, toxicology, and early development. Genomic technologies linking effects in functional genes involved in growth, maturation and life history development have been tied to selection resulting from harvest practices. Incorporating new and ever-increasing knowledge of fish genomes is opening a different perspective on local adaptation that will prove invaluable in wild fish conservation and management. Conservation of fish stocks is rapidly incorporating research on critical adaptive responses directed at the effects of human disturbance and climate change through gene expression studies. Genomic studies of fish populations can be generally grouped into three broad categories: 1) evolutionary genomics and biodiversity; 2) adaptive physiological responses to a changing environment; and 3) adaptive behavioral genomics and life history diversity. We review current genomic research in fisheries focusing on those that use microarrays to explore differences in gene expression among phenotypes and within or across populations, information that is critically important to the conservation of fish and their relationship to humans.

Trends toward the Future in Physical Activity Programming

ERIC Educational Resources Information Center

Charles, John M.; Charles, P. Kelly

2016-01-01

In the wake of multiple activity program eliminations and frequent downgrading of activity programs nationwide, the question to be addressed is how we can draw upon our interdisciplinary, intergenerational, and intercultural understandings of human movement to construct physical activity programs for the future that might withstand the test of…

45 CFR 86.31 - Education programs or activities.

Code of Federal Regulations, 2014 CFR

2014-10-01

... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs or activities. (a) General. Except as provided elsewhere in this part, no person shall, on the basis of sex, be...

45 CFR 86.31 - Education programs or activities.

Code of Federal Regulations, 2012 CFR

2012-10-01

... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs or activities. (a) General. Except as provided elsewhere in this part, no person shall, on the basis of sex, be...

45 CFR 86.31 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs or activities. (a) General. Except as provided elsewhere in this part, no person shall, on the basis of sex, be...

17 CFR 200.650 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... the Basis of Handicap in Programs or Activities Conducted by the Securities and Exchange Commission... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

7 CFR 15e.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE UNITED STATES DEPARTMENT OF AGRICULTURE... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

25 CFR 720.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NAVAJO AND HOPI INDIAN RELOCATION... program or activity so that the program or activity, when viewed in its entirety, is readily accessible to... activity or in undue financial and administrative burdens. In those circumstances where agency personnel...

22 CFR 1510.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE AFRICAN DEVELOPMENT FOUNDATION... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

22 CFR 144.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE UNITED STATES DEPARTMENT OF... program or activity so that the program or activity, when viewed in its entirety, is readily accessible to... activity or in undue financial and administrative burdens. In those circumstances where agency personnel...

5 CFR 1850.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE OFFICE OF SPECIAL COUNSEL... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

49 CFR 28.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF TRANSPORTATION § 28... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens...

3 CFR 102.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE EXECUTIVE OFFICE OF THE PRESIDENT... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... the nature of a program or activity or in undue financial and administrative burdens. In those...

29 CFR 4907.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE PENSION BENEFIT GUARANTY CORPORATION § 4907.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable by handicapped...

32 CFR 1699.150 - Program accessibility: existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... SYSTEM ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY SELECTIVE SERVICE SYSTEM § 1699.150 Program accessibility: existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is...

The tailored activity program (TAP) to address behavioral disturbances in frontotemporal dementia: a feasibility and pilot study.

PubMed

O'Connor, Claire M; Clemson, Lindy; Brodaty, Henry; Low, Lee-Fay; Jeon, Yun-Hee; Gitlin, Laura N; Piguet, Olivier; Mioshi, Eneida

2017-10-15

To explore the feasibility of implementing the Tailored Activity Program with a cohort of people with frontotemporal dementia and their carers (dyads). The Tailored Activity Program is an occupational therapy based intervention that involves working collaboratively with family carers and prescribes personalized activities for behavioral management in people with dementia. Twenty dyads randomized into the study (Tailored Activity Program: n = 9; Control: n = 11) were assessed at baseline and 4-months. Qualitative analyzes evaluated feasibility and acceptability of the program for the frontotemporal dementia cohort, and quantitative analyzes (linear mixed model analyzes, Spearman's rho correlations) measured the impact of the program on the dyads. The Tailored Activity Program was an acceptable intervention for the frontotemporal dementia dyads. Qualitative analyses identified five themes: "carer perceived benefits", "carer readiness to change", "strategies used by carer to engage person with dementia", "barriers to the Tailored Activity Program uptake/implementation", and "person with dementia engagement". Quantitative outcomes showed an overall reduction of behavioral symptoms (F 18.34  = 8.073, p = 0.011) and maintenance of functional performance in the person with dementia (F 18.03  = 0.375, p = 0.548). This study demonstrates the potential for using an activity-based intervention such as the Tailored Activity Program in frontotemporal dementia. Service providers should recognize that while people with frontotemporal dementia present with challenging issues, tailored therapies may support their function and reduce their behavioral symptoms. Implications for rehabilitation The Tailored Activity Program is an occupational therapy based intervention that involves prescribing personalized activities for behavioral management in dementia. The Tailored Activity Program is an acceptable and feasible intervention approach to address some of the unique behavioral and functional impairments inherent in frontotemporal dementia.

18 CFR 1317.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Education programs or... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1317.400 Education programs...

22 CFR 1005.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE INTER-AMERICAN FOUNDATION § 1005.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable by...

12 CFR 794.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL CREDIT UNION ADMINISTRATION § 794.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable by handicapped...

50 CFR 550.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY MARINE MAMMAL COMMISSION § 550.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable by...

28 CFR 39.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF JUSTICE § 39.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable by...

18 CFR 1317.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Education programs or... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1317.400 Education programs...

32 CFR 199.16 - Supplemental Health Care Program for active duty members.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Supplemental Health Care Program for active duty... (CHAMPUS) § 199.16 Supplemental Health Care Program for active duty members. (a) Purpose and applicability... the supplemental health care program for active duty members of the uniformed services, the provision...

32 CFR 199.16 - Supplemental Health Care Program for active duty members.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Supplemental Health Care Program for active duty... (CHAMPUS) § 199.16 Supplemental Health Care Program for active duty members. (a) Purpose and applicability... the supplemental health care program for active duty members of the uniformed services, the provision...

32 CFR 199.16 - Supplemental Health Care Program for active duty members.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false Supplemental Health Care Program for active duty... (CHAMPUS) § 199.16 Supplemental Health Care Program for active duty members. (a) Purpose and applicability... the supplemental health care program for active duty members of the uniformed services, the provision...

32 CFR 199.16 - Supplemental Health Care Program for active duty members.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 2 2013-07-01 2013-07-01 false Supplemental Health Care Program for active duty... (CHAMPUS) § 199.16 Supplemental Health Care Program for active duty members. (a) Purpose and applicability... the supplemental health care program for active duty members of the uniformed services, the provision...

32 CFR 199.16 - Supplemental Health Care Program for active duty members.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 2 2012-07-01 2012-07-01 false Supplemental Health Care Program for active duty... (CHAMPUS) § 199.16 Supplemental Health Care Program for active duty members. (a) Purpose and applicability... the supplemental health care program for active duty members of the uniformed services, the provision...

43 CFR 17.550 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... on the Basis of Handicap in Programs or Activities Conducted by the Department of the Interior § 17... activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable... the nature of a program or activity or in undue financial and administrative burdens. In those...

1 CFR 457.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL CAPITAL PLANNING COMMISSION § 457... activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable... can demonstrate would result in a fundamental alteration in the nature of a program or activity or in...

Active Families in the Great Outdoors: A Program to Promote Family Outdoor Physical Activity

ERIC Educational Resources Information Center

Flynn, Jennifer I.; Bassett, David R.; Fouts, Hillary N.; Thompson, Dixie L.; Coe, Dawn P.

2017-01-01

This study evaluated a 4-week program to increase the time families spent engaging in outdoor activity. Parents were provided strategies to increase family outdoor activity and locations to be active. Sixteen families completed the program. Duration and number of family outdoor activity bouts per week, type of activities, locations, and family…

34 CFR 403.70 - How must funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2010 CFR

2010-07-01

... State Leadership Activities? 403.70 Section 403.70 Education Regulations of the Offices of the... the Basic Programs? State Programs and State Leadership Activities § 403.70 How must funds be used under the State Programs and State Leadership Activities? A State shall use funds reserved under section...

34 CFR 403.70 - How must funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2012 CFR

2012-07-01

... State Leadership Activities? 403.70 Section 403.70 Education Regulations of the Offices of the... the Basic Programs? State Programs and State Leadership Activities § 403.70 How must funds be used under the State Programs and State Leadership Activities? A State shall use funds reserved under section...

34 CFR 403.70 - How must funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2011 CFR

2011-07-01

... State Leadership Activities? 403.70 Section 403.70 Education Regulations of the Offices of the... the Basic Programs? State Programs and State Leadership Activities § 403.70 How must funds be used under the State Programs and State Leadership Activities? A State shall use funds reserved under section...

34 CFR 403.70 - How must funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2014 CFR

2014-07-01

... State Leadership Activities? 403.70 Section 403.70 Education Regulations of the Offices of the... the Basic Programs? State Programs and State Leadership Activities § 403.70 How must funds be used under the State Programs and State Leadership Activities? A State shall use funds reserved under section...

34 CFR 403.70 - How must funds be used under the State Programs and State Leadership Activities?

Code of Federal Regulations, 2013 CFR

2013-07-01

... State Leadership Activities? 403.70 Section 403.70 Education Regulations of the Offices of the... the Basic Programs? State Programs and State Leadership Activities § 403.70 How must funds be used under the State Programs and State Leadership Activities? A State shall use funds reserved under section...

49 CFR 17.3 - What programs and activities of the Department are subject to these regulations?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false What programs and activities of the Department are subject to these regulations? 17.3 Section 17.3 Transportation Office of the Secretary of Transportation INTERGOVERNMENTAL REVIEW OF DEPARTMENT OF TRANSPORTATION PROGRAMS AND ACTIVITIES § 17.3 What programs and activities...

Scholarly Activities of Family Medicine Faculty: Results of a National Survey.

PubMed

Hinojosa, Jose; Benè, Kristen L; Hickey, Colleen; Marvel, Kim

2006-12-01

This survey examined how family medicine residency programs define scholarly activity, the productivity of programs, and perceived barriers to scholarly work. Five types of residency programs are compared: university-based, community-based (unaffiliated, university-affiliated, university-administered), and military. A 13 item web-based questionnaire was sent to all 455 U. S. family medicine residency programs. The survey solicited demographic information as well as program expectations of faculty, presence of a research coordinator/director, activities considered scholarly, productivity, and perceived barriers. A total of 177 surveys were completed for a response rate of 38%, similar to response rates of web-based surveys in the literature. 67.6% of programs encouraged, but did not require scholarly activity, and 44.5% indicated their program had no research coordinator/ director. University-based programs had the highest levels of productivity compared to other program types. Primary barriers to scholarly activity noted were lack of time (73/138, 53%) and lack of supportive infrastructure (37/138, 27%). While interpretations are limited by the response rate of the survey, results provide an increased understanding of how programs define scholarly activity as well as reference points for faculty productivity. This information can help program directors when setting criteria for scholarly work.

Research training among pediatric residency programs: a national assessment.

PubMed

Abramson, Erika L; Naifeh, Monique M; Stevenson, Michelle D; Todd, Christopher; Henry, Emilie D; Chiu, Ya-Lin; Gerber, Linda M; Li, Su-Ting T

2014-12-01

The Accreditation Council for Graduate Medical Education (ACGME) states that "residents should participate in scholarly activity." However, there is little guidance for effectively integrating scholarly activity into residency. This study was conducted to understand how pediatric residency programs meet ACGME requirements and to identify characteristics of successful programs. The authors conducted an online cross-sectional survey of all pediatric residency program directors in October 2012, assessing program characteristics, resident participation in scholarly activity, program infrastructure, barriers, and outcomes. Multivariate logistic regression was used to identify characteristics of programs in the top quartile for resident scholarly activity participation. The response rate was 52.8% (105/199 programs). Seventy-seven (78.6%) programs required scholarly activity, although definitions were variable. When including only original research, systematic reviews or meta-analyses, and case reports or series with references, resident participation averaged 56% (range 0%-100%). Characteristics associated with high-participation programs included a scholarly activity requirement (odds ratio [OR] = 5.5, 95% confidence interval [CI] = 1.03-30.0); program director belief that all residents should present work regionally or nationally (OR = 4.7, 95% CI = 1.5-15.1); and mentorship by >25% of faculty (OR = 3.6, CI = 1.2-11.4). Only 47.1% (41) of program directors were satisfied with resident participation, and only 30.7% (27) were satisfied with the quality of research training provided. The findings suggest that resident scholarly activity experience is highly variable and suboptimal. Identifying characteristics of successful programs can improve the resident research training experience.

The Use of Refundable Tax Credits to Increase Low-Income Children's After-School Physical Activity Level.

PubMed

Dunton, Genevieve; Ebin, Vicki J; Efrat, Merav W; Efrat, Rafael; Lane, Christianne J; Plunkett, Scott

2015-06-01

The current study investigates the extent to which a refundable tax credit could be used to increase low-income children's after-school physical activity levels. An experimental study was conducted evaluating the effectiveness of an intervention offering a simulated refundable tax credit to parents of elementary-school-age children (n = 130) for enrollment in after-school physical activity programs. A randomized controlled design was used, with data collected at baseline, immediately following the 4-month intervention (postintervention), and 6 weeks after the end of the intervention (follow-up). Evaluation measures included (1) enrollment rate, time spent, weekly participation frequency, duration of enrollment, and long-term enrollment patterns in after-school physical activity programs and (2) moderate to vigorous physical activity. The simulated tax credits did not significantly influence low-income children's rates of enrollment in after-school physical activity programs, frequency of participation, time spent in after-school physical activity programs, and overall moderate-to-vigorous intensity physical activity at postintervention or follow-up. The use of refundable tax credits as incentives to increase participation in after-school physical activity programs in low-income families may have limited effectiveness. Lawmakers might consider other methods of fiscal policy to promote physical activity such as direct payment to after-school physical activity program providers for enrolling and serving a low-income child in a qualified program, or improvements to programming and infrastructure.

Complementary interactions between command-like interneurons that function to activate and specify motor programs.

PubMed

Wu, Jin-Sheng; Wang, Nan; Siniscalchi, Michael J; Perkins, Matthew H; Zheng, Yu-Tong; Yu, Wei; Chen, Song-an; Jia, Ruo-nan; Gu, Jia-Wei; Qian, Yi-Qing; Ye, Yang; Vilim, Ferdinand S; Cropper, Elizabeth C; Weiss, Klaudiusz R; Jing, Jian

2014-05-07

Motor activity is often initiated by a population of command-like interneurons. Command-like interneurons that reliably drive programs have received the most attention, so little is known about how less reliable command-like interneurons may contribute to program generation. We study two electrically coupled interneurons, cerebral-buccal interneuron-2 (CBI-2) and CBI-11, which activate feeding motor programs in the mollusk Aplysia californica. Earlier work indicated that, in rested preparations, CBI-2, a powerful activator of programs, can trigger ingestive and egestive programs. CBI-2 reliably generated ingestive patterns only when it was repeatedly stimulated. The ability of CBI-2 to trigger motor activity has been attributed to the two program-promoting peptides it contains, FCAP and CP2. Here, we show that CBI-11 differs from CBI-2 in that it contains FCAP but not CP2. Furthermore, it is weak in its ability to drive programs. On its own, CBI-11 is therefore less effective as a program activator. When it is successful, however, CBI-11 is an effective specifier of motor activity; that is, it drives mostly ingestive programs. Importantly, we found that CBI-2 and CBI-11 complement each other's actions. First, prestimulation of CBI-2 enhanced the ability of CBI-11 to drive programs. This effect appears to be partly mediated by CP2. Second, coactivation of CBI-11 with CBI-2 makes CBI-2 programs immediately ingestive. This effect may be mediated by specific actions that CBI-11 exerts on pattern-generating interneurons. Therefore, different classes of command-like neurons in a motor network may make distinct, but potentially complementary, contributions as either activators or specifiers of motor activity.

Complementary Interactions between Command-Like Interneurons that Function to Activate and Specify Motor Programs

PubMed Central

Wu, Jin-Sheng; Wang, Nan; Siniscalchi, Michael J.; Perkins, Matthew H.; Zheng, Yu-Tong; Yu, Wei; Chen, Song-an; Jia, Ruo-nan; Gu, Jia-Wei; Qian, Yi-Qing; Ye, Yang; Vilim, Ferdinand S.; Cropper, Elizabeth C.; Weiss, Klaudiusz R.

2014-01-01

Motor activity is often initiated by a population of command-like interneurons. Command-like interneurons that reliably drive programs have received the most attention, so little is known about how less reliable command-like interneurons may contribute to program generation. We study two electrically coupled interneurons, cerebral-buccal interneuron-2 (CBI-2) and CBI-11, which activate feeding motor programs in the mollusk Aplysia californica. Earlier work indicated that, in rested preparations, CBI-2, a powerful activator of programs, can trigger ingestive and egestive programs. CBI-2 reliably generated ingestive patterns only when it was repeatedly stimulated. The ability of CBI-2 to trigger motor activity has been attributed to the two program-promoting peptides it contains, FCAP and CP2. Here, we show that CBI-11 differs from CBI-2 in that it contains FCAP but not CP2. Furthermore, it is weak in its ability to drive programs. On its own, CBI-11 is therefore less effective as a program activator. When it is successful, however, CBI-11 is an effective specifier of motor activity; that is, it drives mostly ingestive programs. Importantly, we found that CBI-2 and CBI-11 complement each other's actions. First, prestimulation of CBI-2 enhanced the ability of CBI-11 to drive programs. This effect appears to be partly mediated by CP2. Second, coactivation of CBI-11 with CBI-2 makes CBI-2 programs immediately ingestive. This effect may be mediated by specific actions that CBI-11 exerts on pattern-generating interneurons. Therefore, different classes of command-like neurons in a motor network may make distinct, but potentially complementary, contributions as either activators or specifiers of motor activity. PMID:24806677

UWALK: the development of a multi-strategy, community-wide physical activity program.

PubMed

Jennings, Cally A; Berry, Tanya R; Carson, Valerie; Culos-Reed, S Nicole; Duncan, Mitch J; Loitz, Christina C; McCormack, Gavin R; McHugh, Tara-Leigh F; Spence, John C; Vallance, Jeff K; Mummery, W Kerry

2017-03-01

UWALK is a multi-strategy, multi-sector, theory-informed, community-wide approach using e and mHealth to promote physical activity in Alberta, Canada. The aim of UWALK is to promote physical activity, primarily via the accumulation of steps and flights of stairs, through a single over-arching brand. This paper describes the development of the UWALK program. A social ecological model and the social cognitive theory guided the development of key strategies, including the marketing and communication activities, establishing partnerships with key stakeholders, and e and mHealth programs. The program promotes the use of physical activity monitoring devices to self-monitor physical activity. This includes pedometers, electronic devices, and smartphone applications. In addition to entering physical activity data manually, the e and mHealth program provides the function for objective data to be automatically uploaded from select electronic devices (Fitbit®, Garmin and the smartphone application Moves) The RE-AIM framework is used to guide the evaluation of UWALK. Funding for the program commenced in February 2013. The UWALK brand was introduced on April 12, 2013 with the official launch, including the UWALK website on September 20, 2013. This paper describes the development and evaluation framework of a physical activity promotion program. This program has the potential for population level dissemination and uptake of an ecologically valid physical activity promotion program that is evidence-based and theoretically framed.

Differential Programming Needs of College Students Preferring Web-Based Versus In-Person Physical Activity Programs.

PubMed

Goldstein, Stephanie P; Forman, Evan M; Butryn, Meghan L; Herbert, James D

2017-09-21

College students report several barriers to exercise, highlighting a need for university-based programs that address these challenges. In contrast to in-person interventions, several web-based programs have been developed to enhance program engagement by increasing ease of access and lowering the necessary level of commitment to participate. Unfortunately, web-based programs continue to struggle with engagement and less-than-ideal outcomes. One explanation for this discrepancy is that different intervention modalities may attract students with distinctive activity patterns, motivators, barriers, and program needs. However, no studies have formally evaluated intervention modality preference (e.g., web-based or in-person) among college students. The current study sought to examine the relationship between intervention modality preference and physical activity programming needs. Undergraduate students (n = 157) enrolled in psychology courses at an urban university were asked to complete an online survey regarding current activity patterns and physical activity program preferences. Participants preferring web-based physical activity programs exercised less (p = .05), were less confident in their abilities to exercise (p = .01), were less likely to endorse the maintenance stage of change (p < .01) and perceived more barriers to exercising (p < .01) than those who preferred in-person programming. Findings suggest that students preferring web-based programming may require programs that enhance self-efficacy by fostering goal-setting and problem-solving skills. A user-centered design approach may enhance the engagement (and therefore effectiveness) of physical activity promotion programs for college students.

10 CFR 4.550 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE FROM THE COMMISSION Enforcement of Nondiscrimination on the Basis of Handicap in Programs or Activities Conducted by the U.S. Nuclear Regulatory Commission § 4.550 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity...

Promoting Physical Activity With the Out of School Nutrition and Physical Activity (OSNAP) Initiative: A Cluster-Randomized Controlled Trial.

PubMed

Cradock, Angie L; Barrett, Jessica L; Giles, Catherine M; Lee, Rebekka M; Kenney, Erica L; deBlois, Madeleine E; Thayer, Julie C; Gortmaker, Steven L

2016-02-01

Millions of children attend after-school programs in the United States. Increasing physical activity levels of program participants could have a broad effect on children's health. To test the effectiveness of the Out of School Nutrition and Physical Activity (OSNAP) Initiative in increasing children's physical activity levels in existing after-school programs. Cluster-randomized controlled trial with matched program pairs. Baseline data were collected September 27 through November 12, 2010, with follow-up data collected April 25 through May 27, 2011. The dates of our analysis were March 11, 2014, through August 18, 2015. The setting was 20 after-school programs in Boston, Massachusetts. All children 5 to 12 years old in participating programs were eligible for study inclusion. Ten programs participated in a series of three 3-hour learning collaborative workshops, with additional optional opportunities for training and technical assistance. Change in number of minutes and bouts of moderate to vigorous physical activity, vigorous physical activity, and sedentary activity and change in total accelerometer counts between baseline and follow-up. Participants with complete data were 402 racially/ethnically diverse children, with a mean age of 7.7 years. Change in the duration of physical activity opportunities offered to children during program time did not differ between conditions (-1.2 minutes; 95% CI, -14.2 to 12.4 minutes; P = .87). Change in moderate to vigorous physical activity minutes accumulated by children during program time did not differ significantly by intervention status (-1.0; 95% CI, -3.3 to 1.3; P = .40). Total minutes per day of vigorous physical activity (3.2; 95% CI, 1.8-4.7; P < .001), vigorous physical activity minutes in bouts (4.1; 95% CI, 2.7-5.6; P < .001), and total accelerometer counts per day (16,894; 95% CI, 5101-28,686; P = .01) increased significantly during program time among intervention participants compared with control participants. Although programs participating in the OSNAP Initiative did not allot significantly more time for physical activity, they successfully made existing time more vigorously active for children receiving the intervention. clinicaltrials.gov Identifier: NCT01396473.

36 CFR 1211.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Education programs or... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities...

38 CFR 23.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 23...

43 CFR 41.400 - Education programs or activities.

Code of Federal Regulations, 2014 CFR

2014-10-01

... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 41.400 Education programs or... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected to...

40 CFR 5.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 5.400 Education programs or... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected to...

43 CFR 41.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 41.400 Education programs or... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected to...

36 CFR 1208.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... OR ACTIVITIES CONDUCTED BY THE NATIONAL ARCHIVES AND RECORDS ADMINISTRATION § 1208.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable by individuals with...

45 CFR 2490.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... ACTIVITIES CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION § 2490.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable by individuals with handicaps...

38 CFR 23.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 23...

36 CFR 1211.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Education programs or... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities...

Physical activity promotion among underserved adolescents: "make it fun, easy, and popular".

PubMed

Louise Bush, Paula; Laberge, Suzanne; Laforest, Sophie

2010-05-01

There is a paucity of studies regarding noncurricular physical activity promotion interventions among adolescents, and even less such research pertaining to underserved youth. This article describes the development and implementation of a noncurricular, school-based physical activity promotion program designed for a multiethnic, underserved population of adolescents. The program's impact on leisure-time physical activity (LTPA) and on physical activity enjoyment (PAE) is also presented. The 16-week program, named FunAction, utilizes social marketing principles. Control (n = 90) and intervention (n = 131) students are assessed pre- and postintervention for levels of LTPA and PAE. Results indicate that although the program did not contribute to an increase in LTPA or PAE among intervention group students, participation in the program was elevated. This study offers preliminary evidence that noncurricular physical activity promotion programs that apply social marketing principles can be effective in engaging multiethnic, underserved adolescents in physical activity.

Active Learning Crosses Generations.

ERIC Educational Resources Information Center

Woodard, Diane K.

2002-01-01

Describes the benefits of intergenerational programs, highlighting a child care program that offers age-appropriate and mutually beneficial activities for children and elders within a nearby retirement community. The program has adopted High/Scope's active learning approach to planning and implementing activities that involve both generations. The…

Let’s Move for Pacific Islander Communities: An Evidence-Based Intervention to Increase Physical Activity

PubMed Central

LaBreche, Mandy; Cheri, Ashley; Custodio, Harold; Fex, Cleo Carlos; Foo, Mary Anne; Lepule, Jonathan Tana; May, Vanessa Tui’one; Orne, Annette; Pang, Jane Ka’ala; Pang, Victor Kaiwi; Sablan-Santos, Lola; Schmidt-Vaivao, Dorothy; Surani, Zul; Talavou, Melevesi Fifita; Toilolo, Tupou; Palmer, Paula Healani; Tanjasiri, Sora Park

2015-01-01

Pacific Islander (PI) populations of Southern California experience high obesity and low physical activity levels. Given PI’s rich cultural ties, efforts to increase physical activity using a community tailored strategy may motivate members in a more sustainable manner. In this paper, we: 1) detail the program adaptation methodology that was utilized to develop the Weaving an Islander Network for Cancer Awareness, Research and Training (WINCART) Center’s PI Let’s Move Program, a culturally-tailored program aimed to increase physical activity levels among members of PI organizations in Southern California, and 2) share the program’s pilot evaluation results on individual and organizational changes. The WINCART Center applied the National Cancer Institute’s program adaptation guidelines to tailor the evidence-based Instant Recess program to fit the needs of PIs. The end product, the PI Let’s Move Program, was piloted in 2012 with eight PI organizations, reaching 106 PI adults. At baseline, 52% of participants reported that they were not physically active, with the average number of days engaged in medium-intensity physical activity at 2.09 days/week. After the 2-month program, participants increased the number of days that they engaged in medium-intensity physical activity from 2.09 to 2.90 days/week. Post-pilot results found that 82% of participants reported intentions to engage in physical activity for at least the next six months. At baseline, only one organization was currently implementing a physical activity program, and none had implemented an evidence-based physical activity program tailored for PIs. After the 2-month timeframe, despite varying levels of capacity, all eight organizations were able to successfully implement the program. In conclusion, results from our program provide evidence that disparity populations, such as PIs, can be successfully reached through programs that are culturally tailored to both individuals and their community organizations. PMID:26153489

10 CFR 5.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Education programs or activities. 5.400 Section 5.400 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

7 CFR 15a.31 - Education programs and activities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 1 2011-01-01 2011-01-01 false Education programs and activities. 15a.31 Section 15a.31 Agriculture Office of the Secretary of Agriculture EDUCATION PROGRAMS OR ACTIVITIES RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs and...

45 CFR 618.400 - Education programs or activities.

Code of Federal Regulations, 2014 CFR

2014-10-01

... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 618.400 Education programs or... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected to...

10 CFR 5.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Education programs or activities. 5.400 Section 5.400 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

29 CFR 36.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 1 2010-07-01 2010-07-01 true Education programs or activities. 36.400 Section 36.400 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

7 CFR 15a.31 - Education programs and activities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Education programs and activities. 15a.31 Section 15a.31 Agriculture Office of the Secretary of Agriculture EDUCATION PROGRAMS OR ACTIVITIES RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs and...

Exploring Extension Involvement in Farm to School Program Activities

ERIC Educational Resources Information Center

Benson, Matthew C.

2014-01-01

The study reported here examined Extension professionals' involvement in farm-to-school program activities. Results of an online survey distributed to eight state Extension systems indicate that on average, Extension professionals are involved with one farm to school program activity, with most supporting school or community garden programs.…

Effects of a pediatric weight management program with and without active video games a randomized trial.

PubMed

Trost, Stewart G; Sundal, Deborah; Foster, Gary D; Lent, Michelle R; Vojta, Deneen

2014-05-01

Active video games may offer an effective strategy to increase physical activity in overweight and obese children. However, the specific effects of active gaming when delivered within the context of a pediatric weight management program are unknown. To evaluate the effects of active video gaming on physical activity and weight loss in children participating in an evidence-based weight management program delivered in the community. Group-randomized clinical trial conducted during a 16-week period in YMCAs and schools located in Massachusetts, Rhode Island, and Texas. Seventy-five overweight or obese children (41 girls [55%], 34 whites [45%], 20 Hispanics [27%], and 17 blacks [23%]) enrolled in a community-based pediatric weight management program. Mean (SD) age of the participants was 10.0 (1.7) years; body mass index (BMI) z score, 2.15 (0.40); and percentage overweight from the median BMI for age and sex, 64.3% (19.9%). All participants received a comprehensive family-based pediatric weight management program (JOIN for ME). Participants in the program and active gaming group received hardware consisting of a game console and motion capture device and 1 active game at their second treatment session and a second game in week 9 of the program. Participants in the program-only group were given the hardware and 2 games at the completion of the 16-week program. Objectively measured daily moderate-to-vigorous and vigorous physical activity, percentage overweight, and BMI z score. Participants in the program and active gaming group exhibited significant increases in moderate-to-vigorous (mean [SD], 7.4 [2.7] min/d) and vigorous (2.8 [0.9] min/d) physical activity at week 16 (P < .05). In the program-only group, a decline or no change was observed in the moderate-to-vigorous (mean [SD] net difference, 8.0 [3.8] min/d; P = .04) and vigorous (3.1 [1.3] min/d; P = .02) physical activity. Participants in both groups exhibited significant reductions in percentage overweight and BMI z scores at week 16. However, the program and active gaming group exhibited significantly greater reductions in percentage overweight (mean [SD], -10.9%[1.6%] vs -5.5%[1.5%]; P = .02) and BMI z score (-0.25 [0.03] vs -0.11 [0.03]; P < .001). CONCLUSIONS AND RELEVANCE Incorporating active video gaming into an evidence-based pediatric weight management program has positive effects on physical activity and relative weight. TRIAL REGISTRATION clinicaltrials.gov Identifier: NCT01757925.

The Use of Refundable Tax Credits to Increase Low-Income Children's After-School Physical Activity Level

PubMed Central

Dunton, Genevieve; Ebin, Vicki J.; Efrat, Merav W.; Efrat, Rafael; Lane, Christianne J.; Plunkett, Scott

2014-01-01

Objective The present study investigates the extent to which a refundable tax credit could be used to increase low income children's after-school physical activity levels. Methods An experimental study was conducted evaluating the effectiveness of an intervention offering a simulated refundable tax credit to parents of elementary school-age children (n=130) for enrollment in after-school physical activity programs. A randomized-controlled design was used, with data collected at baseline, immediately following the four month intervention (post-intervention), and six-weeks after the end of the intervention (follow-up). Evaluation measures included: (a) enrollment rate, time spent, weekly participation frequency, duration of enrollment and long term enrollment patterns in after-school physical activity programs; and (b) moderate-to-vigorous physical activity (MVPA). Results The simulated tax credits did not significantly influence low- income children's rates of enrollment in after-school physical activity programs, frequency of participation, time spent in after-school physical activity programs, and overall moderate-to-vigorous intensity physical activity at post-intervention or follow-up. Conclusion The use of refundable tax credits as incentives to increase participation in after-school physical activity programs in low-income families may have limited effectiveness. Lawmakers might consider other methods of fiscal policy to promote physical activity such as direct payment to after-school physical activity program providers for enrolling and serving a low- income child in a qualified program, or improvements to programming and infrastructure. PMID:25184738

A Beginner's Sequence of Programming Activities.

ERIC Educational Resources Information Center

Slesnick, Twila

1984-01-01

Presents various programing activities using the BASIC and LOGO programing languages. Activities are included in separate sections with a title indicating the nature of the activities and the "tools" (commands) needed. For example, "Old-fashioned drawing" requires several tools (PRINT, LIST, RUN, GOTO) to make drawings using…

Comprehensive School Physical Activity Programs: Recommendations for Physical Education Teacher Education

ERIC Educational Resources Information Center

Zhang, Xiaoxia; Gu, Xiangli; Zhang, Tao; Keller, Jean; Chen, Senlin

2018-01-01

Comprehensive school physical activity programs (CSPAPs) aim to promote physical activity and healthy lifestyles among school-age children and adolescents. Physical educators are highly qualified individuals taking on the role of certified physical activity leaders. Physical education teacher education (PETE) programs should consider preparing…

22 CFR 530.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE BROADCASTING BOARD OF GOVERNORS... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... activity or in undue financial and administrative burdens. In those circumstances where Board personnel...

46 CFR 507.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE FEDERAL MARITIME COMMISSION... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... activity or in undue financial and administrative burdens. In those circumstances where agency personnel...

1 CFR 500.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL COMMISSION FOR EMPLOYMENT POLICY... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... activity or in undue financial and administrative burdens. In those circumstances where agency personnel...

49 CFR 1014.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE SURFACE TRANSPORTATION BOARD... or activity so that the program or activity, when viewed in its entirety, is readily accessible to... activity or in undue financial and administrative burdens. In those circumstances where agency personnel...

48 CFR 719.271-4 - Heads of contracting activities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... activities. 719.271-4 Section 719.271-4 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities. In order for the agency small business program to be effective, the active support of top management...

Playtime Is Science: Implementing a Parent/Child Activity Program.

ERIC Educational Resources Information Center

Sprung, Barbara; And Others

A program of science activities for children in the early childhood years and their parents is offered. The three different formats of the Playtime Is Science program are adaptable to a variety of settings and schedules. The Parent/Child Activity Program includes one parents-only session in which participants learn that routine chores involve…

Cultivating Leadership, Pedagogy and Programming for CSPAP and Healthy, Active Lifestyles at the University of Idaho

ERIC Educational Resources Information Center

Goc Karp, Grace; Brown, Helen; Scruggs, Philip W.; Berei, Catherine

2017-01-01

This article highlights processes for infusing comprehensive school physical activity programming (CSPAP) into the physical education teacher education (PETE) program at the University of Idaho (UI). The PETE program uses a modified leadership framework to target learning outcomes and activities pertinent to CSPAP. Student CSPAP knowledge and…

Montessori-based activities for long-term care residents with advanced dementia: effects on engagement and affect.

PubMed

Orsulic-Jeras, S; Judge, K S; Camp, C J

2000-02-01

Sixteen residents in long-term care with advanced dementia (14 women; average age = 88) showed significantly more constructive engagement (defined as motor or verbal behaviors in response to an activity), less passive engagement (defined as passively observing an activity), and more pleasure while participating in Montessori-based programming than in regularly scheduled activities programming. Principles of Montessori-based programming, along with examples of such programming, are presented. Implications of the study and methods for expanding the use of Montessori-based dementia programming are discussed.

Best-Practice Physical Activity Programs for Older Adults: Findings From the National Impact Study

PubMed Central

Seymour, Rachel B.; Campbell, Richard T.; Whitelaw, Nancy; Bazzarre, Terry

2009-01-01

Objectives. We assessed the impact of existing best-practice physical activity programs for older adults on physical activity participation and health-related outcomes. Methods. We used a multisite, randomized trial with 544 older adults (mean age 66 years) and measures at baseline, 5, and 10 months to test the impact of a multiple-component physical activity program compared with results for a control group that did not participate in such a program. Results. For adults who participated in a multiple-component physical activity program, we found statistically significant benefits at 5 and 10 months with regard to self-efficacy for exercise adherence over time (P < .001), adherence in the face of barriers (P = .01), increased upper- and lower-body strength (P = .02, P = .01), and exercise participation (P = .01). Conclusions. Best-practice community-based physical activity programs can measurably improve aspects of functioning that are risk factors for disability among older adults. US public policy should encourage these inexpensive health promotion programs. PMID:19059858

Girls Just Wanna Have Fun: a process evaluation of a female youth-driven physical activity-based life skills program.

PubMed

Bean, Corliss N; Forneris, Tanya; Halsall, Tanya

2014-01-01

Integrating a positive youth development framework into physical activity programming has become popular as it is believed that this integration can create the development of both physical and psychosocial skills. However, there has been a lack of intervention fidelity research within the field of positive youth development. The Girls Just Wanna Have Fun program was designed in response to increased calls for physical activity programs for female youth and is a theoretically-grounded physical activity-based life skills program that aims to empower female youth. The purpose of this paper was to provide a detailed description of the program and a process evaluation of the first year of program implementation. From interviews with youth and leaders, as well as documentation from the leaders' weekly online log of each implemented session, themes emerged regarding the successes. Findings from this study indicated that program goals were attained and it appears that the program was implemented, for the most part, as designed. The themes related to successes included using activities to facilitate relational time, providing intentional opportunities for leadership, having communicative program leaders who supported one another, and engaging youth in different types of physical activity. The themes related to challenges included difficulties with facility and transportation, some activities being too much like schoolwork, and social distractions and cliques. Included in the paper is a discussion of practical implications and recommendations for community programmers, as well as future directions for the program. Overall, this process evaluation represents an important step in responding to calls for increased evaluation in community-based programs and aids in understanding the process in which positive youth development programs can be effectively implemented.

Assessing program efficiency: a time and motion study of the Mental Health Emergency Care - Rural Access Program in NSW Australia.

PubMed

Saurman, Emily; Lyle, David; Kirby, Sue; Roberts, Russell

2014-07-31

The Mental Health Emergency Care-Rural Access Program (MHEC-RAP) is a telehealth solution providing specialist emergency mental health care to rural and remote communities across western NSW, Australia. This is the first time and motion (T&M) study to examine program efficiency and capacity for a telepsychiatry program. Clinical services are an integral aspect of the program accounting for 6% of all activities and 50% of the time spent conducting program activities, but half of this time is spent completing clinical paperwork. This finding emphasizes the importance of these services to program efficiency and the need to address variability of service provision to impact capacity. Currently, there is no efficiency benchmark for emergency telepsychiatry programs. Findings suggest that MHEC-RAP could increase its activity without affecting program responsiveness. T&M studies not only determine activity and time expenditure, but have a wider application assessing program efficiency by understanding, defining, and calculating capacity. T&M studies can inform future program development of MHEC-RAP and similar telehealth programs, both in Australia and overseas.

Active for Life After Cancer: a randomized trial examining a lifestyle physical activity program for prostate cancer patients.

PubMed

Carmack Taylor, Cindy L; Demoor, Carl; Smith, Murray A; Dunn, Andrea L; Basen-Engquist, Karen; Nielsen, Ingrid; Pettaway, Curtis; Sellin, Rena; Massey, Pamela; Gritz, Ellen R

2006-10-01

Active for Life After Cancer is a randomized trial evaluating the efficacy of a 6-month group-based lifestyle physical activity program (Lifestyle) for prostate cancer patients to improve quality of life (QOL) including physical and emotional functioning compared to a group-based Educational Support Program and a Standard Care Program (no group). A total of 134 prostate cancer patients receiving continuous androgen-ablation were randomly assigned to one of the three study conditions. Results indicated no significant improvements in QOL at 6 or 12 months. Both group-based programs were positively received and yielded good attendance and retention. Lifestyle participants demonstrated significant improvements in most theoretical mediators proposed by the Transtheoretical Model and Social Cognitive Theory to affect physical activity. Despite these improvements, no significant changes were found for most physical activity measures. Results suggest a lifestyle program focusing on cognitive-behavioral skills training alone is insufficient for promoting routine physical activity in these patients.

10 CFR 1040.63 - Discrimination prohibited.

Code of Federal Regulations, 2010 CFR

2010-01-01

... subjected to discrimination under any program or activity that receives Federal financial assistance from... or substantially impairing accomplishment of the objectives of the recipient's program or activity... subjecting them to discrimination under any program or activity that receives Federal financial assistance...

28 CFR 42.503 - Discrimination prohibited.

Code of Federal Regulations, 2010 CFR

2010-07-01

... program or activity receiving Federal financial assistance. (b) Discriminatory actions prohibited. (1) A... program or activity receiving Federal financial assistance; (ii) Deny a qualified handicapped person an... program or activity receiving Federal financial assistance on the ground that other specialized aid...

Health-Related Quality of Life, Self-Efficacy and Enjoyment Keep the Socially Vulnerable Physically Active in Community-Based Physical Activity Programs: A Sequential Cohort Study

PubMed Central

Herens, Marion; Bakker, Evert Jan; van Ophem, Johan; Wagemakers, Annemarie; Koelen, Maria

2016-01-01

Physical inactivity is most commonly found in socially vulnerable groups. Dutch policies target these groups through community-based health-enhancing physical activity (CBHEPA) programs. As robust evidence on the effectiveness of this approach is limited, this study investigated whether CBHEPA programs contribute to an increase in and the maintenance of physical activity in socially vulnerable groups. In four successive cohorts, starting at a six-month interval, 268 participants from 19 groups were monitored for twelve months in seven CBHEPA programs. Data collection was based on repeated questionnaires. Socio-economic indicators, program participation and coping ability were measured at baseline. Physical activity, health-related quality of life and on-going program participation were measured three times. Self-efficacy and enjoyment were measured at baseline and at twelve months. Statistical analyses were based on a quasi-RCT design (independent t-tests), a comparison of participants and dropouts (Mann-Whitney test), and multilevel modelling to assess change in individual physical activity, including group level characteristics. Participants of CBHEPA programs are socially vulnerable in terms of low education (48.6%), low income (52.4%), non-Dutch origin (64.6%) and health-related quality of life outcomes. Physical activity levels were not below the Dutch average. No increase in physical activity levels over time was observed. The multilevel models showed significant positive associations between health-related quality of life, self-efficacy and enjoyment, and leisure-time physical activity over time. Short CBHEPA programs (10–13 weeks) with multiple trainers and gender-homogeneous groups were associated with lower physical activity levels over time. At twelve months, dropouts' leisure-time physical activity levels were significantly lower compared to continuing participants, as were health-related quality of life, self-efficacy and enjoyment outcomes. BMI and care consumption scored significantly higher among dropouts. In conclusion, Dutch CBHEPA programs reach socially vulnerable, but not necessarily inactive, groups in terms of socio-economic and health-related quality of life outcomes. Our findings suggest that CBHEPA programs particularly contribute to physical activity maintenance in socially vulnerable groups, rather than to an increase in physical activity behaviour over time. PMID:26909696

Health-Related Quality of Life, Self-Efficacy and Enjoyment Keep the Socially Vulnerable Physically Active in Community-Based Physical Activity Programs: A Sequential Cohort Study.

PubMed

Herens, Marion; Bakker, Evert Jan; van Ophem, Johan; Wagemakers, Annemarie; Koelen, Maria

2016-01-01

Physical inactivity is most commonly found in socially vulnerable groups. Dutch policies target these groups through community-based health-enhancing physical activity (CBHEPA) programs. As robust evidence on the effectiveness of this approach is limited, this study investigated whether CBHEPA programs contribute to an increase in and the maintenance of physical activity in socially vulnerable groups. In four successive cohorts, starting at a six-month interval, 268 participants from 19 groups were monitored for twelve months in seven CBHEPA programs. Data collection was based on repeated questionnaires. Socio-economic indicators, program participation and coping ability were measured at baseline. Physical activity, health-related quality of life and on-going program participation were measured three times. Self-efficacy and enjoyment were measured at baseline and at twelve months. Statistical analyses were based on a quasi-RCT design (independent t-tests), a comparison of participants and dropouts (Mann-Whitney test), and multilevel modelling to assess change in individual physical activity, including group level characteristics. Participants of CBHEPA programs are socially vulnerable in terms of low education (48.6%), low income (52.4%), non-Dutch origin (64.6%) and health-related quality of life outcomes. Physical activity levels were not below the Dutch average. No increase in physical activity levels over time was observed. The multilevel models showed significant positive associations between health-related quality of life, self-efficacy and enjoyment, and leisure-time physical activity over time. Short CBHEPA programs (10-13 weeks) with multiple trainers and gender-homogeneous groups were associated with lower physical activity levels over time. At twelve months, dropouts' leisure-time physical activity levels were significantly lower compared to continuing participants, as were health-related quality of life, self-efficacy and enjoyment outcomes. BMI and care consumption scored significantly higher among dropouts. In conclusion, Dutch CBHEPA programs reach socially vulnerable, but not necessarily inactive, groups in terms of socio-economic and health-related quality of life outcomes. Our findings suggest that CBHEPA programs particularly contribute to physical activity maintenance in socially vulnerable groups, rather than to an increase in physical activity behaviour over time.

A Community-Based Volunteer After-School Activity Program Created for Middle School Students.

ERIC Educational Resources Information Center

Greaser, Thomas C., Jr.

This practicum was designed to provide an after-school activity program to middle school students not engaged in interscholastic sports. Utilizing community volunteers, an enrichment-prevention program that featured 19 different activities in 2 class sessions per week over a 10-week period was developed and implemented. Activities included…

STEM Related After-School Program Activities and Associated Outcomes on Student Learning

ERIC Educational Resources Information Center

Sahin, Alpaslan; Ayar, Mehmet C.; Adiguzel, Tufan

2014-01-01

This study explores the characteristics of after-school program activities at a charter school in the Southeast US highlighting students' experiences with and gains from these after-school program activities. A qualitative case study design was employed to understand students' views and opinions regarding the activities and their learning…

Exercise Level and Energy Expenditure in the Take 10![R] In-Class Physical Activity Program.

ERIC Educational Resources Information Center

Stewart, James A.; Dennison, David A.; Kohl, Harold W., III; Doyle, J. Andrew

2004-01-01

This study evaluated the effectiveness of an innovative, classroom-based physical activity prevention program designed to integrate academic curriculum elements along with a physical activity program in providing moderate-to-vigorous intensity physical activity. A convenience sample of three public school classrooms (one first, third, and fifth…

Do health benefits outweigh the costs of mass recreational programs? An economic analysis of four Ciclovía programs.

PubMed

Montes, Felipe; Sarmiento, Olga L; Zarama, Roberto; Pratt, Michael; Wang, Guijing; Jacoby, Enrique; Schmid, Thomas L; Ramos, Mauricio; Ruiz, Oscar; Vargas, Olga; Michel, Gabriel; Zieff, Susan G; Valdivia, Juan Alejandro; Cavill, Nick; Kahlmeier, Sonja

2012-02-01

One promising public health intervention for promoting physical activity is the Ciclovía program. The Ciclovía is a regular multisectorial community-based program in which streets are temporarily closed for motorized transport, allowing exclusive access to individuals for recreational activities and physical activity. The objective of this study was to conduct an analysis of the cost-benefit ratios of physical activity of the Ciclovía programs of Bogotá and Medellín in Colombia, Guadalajara in México, and San Francisco in the U.S.A. The data of the four programs were obtained from program directors and local surveys. The annual cost per capita of the programs was: U.S. $6.0 for Bogotá, U.S. $23.4 for Medellín, U.S. $6.5 for Guadalajara, and U.S. $70.5 for San Francisco. The cost-benefit ratio for health benefit from physical activity was 3.23-4.26 for Bogotá, 1.83 for Medellín, 1.02-1.23 for Guadalajara, and 2.32 for San Francisco. For the program of Bogotá, the cost-benefit ratio was more sensitive to the prevalence of physically active bicyclists; for Guadalajara, the cost-benefit ratio was more sensitive to user costs; and for the programs of Medellín and San Francisco, the cost-benefit ratios were more sensitive to operational costs. From a public health perspective for promoting physical activity, these Ciclovía programs are cost beneficial.

KENNEDY SPACE CENTER, FLA. -- NASA and United Space Alliance (USA) Space Shuttle program managers attend a briefing, part of activities during a leadership workday. The day is intended to provide management with an in-depth, hands-on look at Shuttle processing activities at KSC. Starting third from left are NASA Deputy Associate Administrator for Space Station and Shuttle Programs Michael Kostelnik, USA Vice President and Space Shuttle Program Manager Howard DeCastro, NASA Space Shuttle Program Manager William Parsons, and USA Associate Program Manager of Ground Operations Andy Allen.

NASA Image and Video Library

2003-12-19

KENNEDY SPACE CENTER, FLA. -- NASA and United Space Alliance (USA) Space Shuttle program managers attend a briefing, part of activities during a leadership workday. The day is intended to provide management with an in-depth, hands-on look at Shuttle processing activities at KSC. Starting third from left are NASA Deputy Associate Administrator for Space Station and Shuttle Programs Michael Kostelnik, USA Vice President and Space Shuttle Program Manager Howard DeCastro, NASA Space Shuttle Program Manager William Parsons, and USA Associate Program Manager of Ground Operations Andy Allen.

Acute toxicity of cadmium, lead, zinc, and their mixtures to stream-resident fish and invertebrates

USGS Publications Warehouse

Mebane, Christopher A.; Dillon, Frank S.; Hennessy, Daniel P.

2012-01-01

The authors conducted 150 tests of the acute toxicity of resident fish and invertebrates to Cd, Pb, and Zn, separately and in mixtures, in waters from the South Fork Coeur d'Alene River watershed, Idaho, USA. Field-collected shorthead sculpin (Cottus confusus), westslope cutthroat trout (Oncorhynchus clarkii lewisi), two mayflies (Baetis tricaudatus and Rhithrogena sp.), a stonefly (Sweltsa sp.), a caddisfly (Arctopsyche sp.), a snail (Gyraulus sp.), and hatchery rainbow trout (Oncorhynchus mykiss), were tested with all three metals. With Pb, the mayflies (Drunella sp., Epeorus sp., and Leptophlebiidae), a Simuliidae black fly, a Chironomidae midge, a Tipula sp. crane fly, a Dytiscidae beetle, and another snail (Physa sp.), were also tested. Adult westslope cutthroat trout were captured to establish a broodstock to provide fry of known ages for testing. With Cd, the range of 96-h median effect concentrations (EC50s) was 0.4 to >5,329μg/L, and the relative resistances of taxa were westslope cutthroat trout ≈ rainbow trout ≈ sculpin << other taxa; with Pb, EC50s ranged from 47 to 3,323μg/L, with westslope cutthroat trout < rainbow trout < other taxa; and with Zn, EC50s ranged from 21 to 3,704μg/L, with rainbow trout < westslope cutthroat trout ≈ sculpin << other taxa. With swim-up trout fry, a pattern of decreasing resistance with increasing fish size was observed. In metal mixtures, the toxicities of the three metals were less than additive on a concentration-addition basis.

Cephalopod culture: current status of main biological models and research priorities.

PubMed

Vidal, Erica A G; Villanueva, Roger; Andrade, José P; Gleadall, Ian G; Iglesias, José; Koueta, Noussithé; Rosas, Carlos; Segawa, Susumu; Grasse, Bret; Franco-Santos, Rita M; Albertin, Caroline B; Caamal-Monsreal, Claudia; Chimal, Maria E; Edsinger-Gonzales, Eric; Gallardo, Pedro; Le Pabic, Charles; Pascual, Cristina; Roumbedakis, Katina; Wood, James

2014-01-01

A recent revival in using cephalopods as experimental animals has rekindled interest in their biology and life cycles, information with direct applications also in the rapidly growing ornamental aquarium species trade and in commercial aquaculture production for human consumption. Cephalopods have high rates of growth and food conversion, which for aquaculture translates into short culture cycles, high ratios of production to biomass and high cost-effectiveness. However, at present, only small-scale culture is possible and only for a few species: the cuttlefish Sepia officinalis, the loliginid squid Sepioteuthis lessoniana and the octopuses Octopus maya and O. vulgaris. These four species are the focus of this chapter, the aims of which are as follows: (1) to provide an overview of the culture requirements of cephalopods, (2) to highlight the physical and nutritional requirements at each phase of the life cycle regarded as essential for successful full-scale culture and (3) to identify current limitations and the topics on which further research is required. Knowledge of cephalopod culture methods is advanced, but commercialization is still constrained by the highly selective feeding habits of cephalopods and their requirement for large quantities of high-quality (preferably live) feed, particularly in the early stages of development. Future research should focus on problems related to the consistent production of viable numbers of juveniles, the resolution of which requires a better understanding of nutrition at all phases of the life cycle and better broodstock management, particularly regarding developments in genetic selection, control of reproduction and quality of eggs and offspring. © 2014 Elsevier Ltd All rights reserved.

Ribosomal genes and heat shock proteins as putative markers for chronic, sublethal heat stress in Arctic charr: applications for aquaculture and wild fish.

PubMed

Quinn, Nicole L; McGowan, Colin R; Cooper, Glenn A; Koop, Ben F; Davidson, William S

2011-09-22

Arctic charr thrive at high densities and can live in freshwater year round, making this species especially suitable for inland, closed containment aquaculture. However, it is a cold-water salmonid, which both limits where the species can be farmed and places wild populations at particular risk to climate change. Previously, we identified genes associated with tolerance and intolerance to acute, lethal temperature stress in Arctic charr. However, there remained a need to examine the genes involved in the stress response to more realistic temperatures that could be experienced during a summer heat wave in grow-out tanks that are not artificially cooled, or under natural conditions. Here, we exposed Arctic charr to sublethal heat stress of 15-18°C for 72 h, and gill tissues extracted before, during (i.e., at 72 h), immediately after cooling and after 72 h of recovery at ambient temperature (6°C) were used for gene expression profiling by microarray and qPCR analyses. The results revealed an expected pattern for heat shock protein expression, which was highest during heat exposure, with significantly reduced expression (approaching control levels) quickly thereafter. We also found that the expression of numerous ribosomal proteins was significantly elevated immediately and 72 h after cooling, suggesting that the gill tissues were undergoing ribosome biogenesis while recovering from damage caused by heat stress. We suggest that these are candidate gene targets for the future development of genetic markers for broodstock development or for monitoring temperature stress and recovery in wild or cultured conditions.

Genetic structure of muskellunge in the Great Lakes region and the effects of supplementation on genetic integrity of wild populations

USGS Publications Warehouse

Turnquist, Keith N.; Larson, Wesley; Farrell, John M.; Hanchin, P.A.; Kapuscinski, Kevin L.; Miller, Loren M.; Scribner, Kim T.; Wilson, Chris C.; Sloss, Brian L.

2017-01-01

Muskellunge (Esox masquinongy) are important apex predators that support numerous recreational fisheries throughout the Great Lakes region. Declines in muskellunge abundance from historical overharvest and environmental degradation have threatened the viability of many populations and prompted significant restoration efforts that often include stocking. The goal of our study was to investigate contemporary population structure and genetic diversity in 42 populations of muskellunge sampled across the Great Lakes region to inform future management and supplementation practices. We genotyped 1896 muskellunge (N = 10–123/population) at 13 microsatellite loci. The greatest genetic variation was between populations of Great Lakes origin and populations of Northern (inland) origin, with both groups also exhibiting significant substructure (overall FST = 0.23). Genetic structure was generally correlated with geography; however, we only found marginal evidence of isolation by distance, likely due to high genetic differentiation among proximate populations. Measures of genetic diversity were moderate across most populations, but some populations displayed low diversity consistent with small population sizes or historical bottlenecks. Many of the populations studied displayed evidence of historic introductions and supplemental stocking, including the presence of individuals with primarily non-native ancestry as well as interlineage hybrids. Our results suggest that the historic population structure of muskellunge is largely intact across the Great Lakes region, but also that stocking practices have altered this structure to some degree. We suggest that future supplementation practices use local sources where possible, and incorporate genetic tools including broodstock screening to ensure that non-native muskellunge are not used to supplement wild populations.

Genetic Characterization of the Fish Piaractus brachypomus by Microsatellites Derived from Transcriptome Sequencing.

PubMed

Jorge, Paulo H; Mastrochirico-Filho, Vito A; Hata, Milene E; Mendes, Natália J; Ariede, Raquel B; de Freitas, Milena Vieira; Vera, Manuel; Porto-Foresti, Fábio; Hashimoto, Diogo T

2018-01-01

The pirapitinga, Piaractus brachypomus (Characiformes, Serrasalmidae), is a fish from the Amazon basin and is considered to be one of the main native species used in aquaculture production in South America. The objectives of this study were: (1) to perform liver transcriptome sequencing of pirapitinga through NGS and then validate a set of microsatellite markers for this species; and (2) to use polymorphic microsatellites for analysis of genetic variability in farmed stocks. The transcriptome sequencing was carried out through the Roche/454 technology, which resulted in 3,696 non-redundant contigs. Of this total, 2,568 contigs had similarity in the non-redundant (nr) protein database (Genbank) and 2,075 sequences were characterized in the categories of Gene Ontology (GO). After the validation process of 30 microsatellite loci, eight markers showed polymorphism. The analysis of these polymorphic markers in farmed stocks revealed that fish farms from North Brazil had a higher genetic diversity than fish farms from Southeast Brazil. AMOVA demonstrated that the highest proportion of variation was presented within the populations. However, when comparing different groups (1: Wild; 2: North fish farms; 3: Southeast fish farms), a considerable variation between the groups was observed. The F ST values showed the occurrence of genetic structure among the broodstocks from different regions of Brazil. The transcriptome sequencing in pirapitinga provided important genetic resources for biological studies in this non-model species, and microsatellite data can be used as the framework for the genetic management of breeding stocks in Brazil, which might provide a basis for a genetic pre-breeding programme.

The Mendelian inheritance of rare flesh and shell colour variants in the black-lipped pearl oyster (Pinctada margaritifera).

PubMed

Ky, Chin-Long; Nakasai, Seiji; Pommier, Steve; Sham Koua, Manaarii; Devaux, Dominique

2016-10-01

Pinctada margaritifera is French Polynesia's most economically important aquaculture species. This pearl oyster has the specific ability to produce cultured pearls with a very wide range of colours, depending on the colour phenotypes of donor oysters used. Its aquaculture is still based on natural spat collection from wild stocks. We investigated three rare colour variants of P. margaritifera - orange flesh, and red and white shell colour phenotypes - in comparison with the wild-type black flesh and shell commonly found in this species. The study aimed to assess the geographic distribution and genetic basis of these colour variants. Colour frequencies were evaluated during transfer and graft processes of pearl oyster seed captured at collector stations. Among the collection locations studied, Mangareva Island showed the highest rate of the orange flesh phenotype, whereas Takaroa and Takume atolls had relatively high rates of red and white shell phenotypes respectively. Broodstocks were made of these rare colour variants, and crosses were performed to produce first- and second-generation progenies to investigate segregation. The results were consistent with Mendelian ratios and suggest a distinct model with no co-dominance: (i) a two-allele model for flesh trait, whereby the orange allele is recessive to the black fleshed type, and (ii) a three-allele model for shell trait, whereby the black wild-type allele is dominant to the red coloration, which is dominant to the white shell. Furthermore, the proposed model provides the basis for producing selected donor pearl oyster lines through hatchery propagation. © 2016 Stichting International Foundation for Animal Genetics.

Comparative susceptibility among three stocks of yellow perch, Perca flavescens (Mitchill), to viral haemorrhagic septicaemia virus strain IVb from the Great Lakes

USGS Publications Warehouse

Olson, W.; Emmenegger, E.; Glenn, J.; Winton, J.; Goetz, F.

2013-01-01

The Great Lakes strain of viral haemorrhagic septicaemia virus IVb (VHSV-IVb) is capable of infecting a wide number of naive species and has been associated with large fish kills in the Midwestern United States since its discovery in 2005. The yellow perch, Perca flavescens (Mitchill), a freshwater species commonly found throughout inland waters of the United States and prized for its high value in sport and commercial fisheries, is a species documented in several fish kills affiliated with VHS. In the present study, differences in survival after infection with VHSV IVb were observed among juvenile fish from three yellow perch broodstocks that were originally derived from distinct wild populations, suggesting innate differences in susceptibility due to genetic variance. While all three stocks were susceptible upon waterborne exposure to VHS virus infection, fish derived from the Midwest (Lake Winnebago, WI) showed significantly lower cumulative % survival compared with two perch stocks derived from the East Coast (Perquimans River, NC and Choptank River, MD) of the United States. However, despite differences in apparent susceptibility, clinical signs did not vary between stocks and included moderate-to-severe haemorrhages at the pelvic and pectoral fin bases and exophthalmia. After the 28-day challenge was complete, VHS virus was analysed in subsets of whole fish that had either survived or succumbed to the infection using both plaque assay and quantitative PCR methodologies. A direct correlation was identified between the two methods, suggesting the potential for both methods to be used to detect virus in a research setting.

Adherence to a Videogame-Based Physical Activity Program for Older Adults with Schizophrenia.

PubMed

Leutwyler, Heather; Hubbard, Erin M; Dowling, Glenna A

2014-08-01

Adults with schizophrenia are a growing segment of the older adult population. Evidence suggests that they engage in limited physical activity. Interventions are needed that are tailored around their unique limitations. An active videogame-based physical activity program that can be offered at a treatment facility can overcome these barriers and increase motivation to engage in physical activity. The purpose of this report is to describe the adherence to a videogame-based physical activity program using the Kinect(®) for Xbox(®) 360 game system (Microsoft(®), Redmond, WA) in older adults with schizophrenia. This was a descriptive longitudinal study among 34 older adults with schizophrenia to establish the adherence to an active videogame-based physical activity program. In our ongoing program, once a week for 6 weeks, participants played an active videogame, using the Kinect for Xbox 360 game system, for 30 minutes. Adherence was measured with a count of sessions attended and with the total minutes attended out of the possible total minutes of attendance (180 minutes). Thirty-four adults with schizophrenia enrolled in the study. The mean number of groups attended was five out of six total (standard deviation=2), and the mean total minutes attended were 139 out of 180 possible (standard deviation=55). Fifty percent had perfect attendance. Older adults with schizophrenia need effective physical activity programs. Adherence to our program suggests that videogames that use the Kinect for Xbox 360 game system are an innovative way to make physical activity accessible to this population.

MLS student active learning within a "cloud" technology program.

PubMed

Tille, Patricia M; Hall, Heather

2011-01-01

In November 2009, the MLS program in a large public university serving a geographically large, sparsely populated state instituted an initiative for the integration of technology enhanced teaching and learning within the curriculum. This paper is intended to provide an introduction to the system requirements and sample instructional exercises used to create an active learning technology-based classroom. Discussion includes the following: 1.) define active learning and the essential components, 2.) summarize teaching methods, technology and exercises utilized within a "cloud" technology program, 3.) describe a "cloud" enhanced classroom and programming 4.) identify active learning tools and exercises that can be implemented into laboratory science programs, and 5.) describe the evaluation and assessment of curriculum changes and student outcomes. The integration of technology in the MLS program is a continual process and is intended to provide student-driven active learning experiences.

[Spontaneous spawning, ontogeny and growth in captivity of Cynoscion squamipinnis (Perciformes: Sciaenidae)].

PubMed

Boza-Abarca, Jorge; Ramírez-Alvarado, Marvin; Barquero-Chanto, Juan; Calvo-Vargas, Emilia; Berrocal-Artavia, Karen

2016-09-01

The croakers or drums are commercial species, which have been overfished in the Nicoya Gulf, Costa Rica. This study aimed to describe, for the first time, the reproduction and the ontogeny of weakfish, Cynoscion squamipinnis in captivity, in order to perform restocking and mariculture proyects. Wild fish (n= 6, 1-2 Kg) were captured and maintained in the Estación de Biología Marina Juan Bertoglia Richards (Puntarenas, Costa Rica) for a two years period (October 2006- December 2008). During this period, maturation stage was monitored periodically by cannula samples in the females (n= 3) and gentle massage in males (n= 3). All fish were stocked in an 18 t tank, with aeration, 33-35 ups of salinity, and a constant temperature (29 ± 1 °C). The spawning period occurred from January to March 2009, producing 162 000 eggs in three spontaneous spawns. The fertilization percentage was 50-60%, and survival after hatching was 60-85%. The egg diameter was 0.852 mm (Standard deviation (SD)= 0.039), and oil drop of 0.269 mm (SD= 0.016). In the embryonary development, the first mitotic division (MD) was observed one hour after spawning (has), the second MD was 1:30 has, the third MD was 2:00 has, the fourth MD was 2:30 has, and fifth MD at 3:00 has. Morule was observed 3:30 has, the blastule 4:30 has, the gastrule 8:30 has, C shape at 10:00 has, and C shape at 12:00 has. After 19 has hatching larvae occurred. The total length (TL) of the larvae was 2.234 mm (SD= 0.122), and the nothochordial length (NL) was 2.179 mm (SD= 0.119). Preflexion stage was observed 49 has, flexion stage was 11 days after spawn (das) (3.767 mm LT (SD= 0.209)), and postflexion stage was 14 das (4.015 mm LT (SD= 0.302)). After 45 das, the juvenile weights 3.68 g (SD= 1.09). Hatch time of the weakfish larvae was minor than of others croaker species. The stages times of embrionary development were a little different from others croaker species, and probably respond to genetic characteristics of each species and the eggs incubation temperature. The spontaneously spawning without broodstock hormonal applications, and the juveniles production in captivity showed that weakfish is a potential species for restocking programs and mariculture projects.

Umatilla River Fish Passage Operations Project : Annual Progress Report October 2007 - September 2008.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bronson, James P.; Loffink, Ken; Duke, Bill

Threemile Falls Dam (Threemile Dam), located near the town of Umatilla, is the major collection and counting point for adult salmonids returning to the Umatilla River. Returning salmon and steelhead were enumerated at Threemile Dam from June 7, 2007 to August 11, 2008. A total of 3,133 summer steelhead (Oncorhynchus mykiss); 1,487 adult, 1,067 jack, and 999 subjack fall Chinook (O. tshawytscha); 5,140 adult and 150 jack coho (O. kisutch); and 2,009 adult, 517 jack, and 128 subjack spring Chinook (O. tshawytscha) were counted. All fish were enumerated at the east bank facility. Of the fish counted, 1,442 summer steelheadmore » and 88 adult and 84 jack spring Chinook were hauled upstream from Threemile Dam. There were 1,497 summer steelhead; 609 adult, 1,018 jack and 979 subjack fall Chinook; 5,036 adult and 144 jack coho; and 1,117 adult, 386 jack and 125 subjack spring Chinook either released at, or allowed to volitionally migrate past, Threemile Dam. Also, 110 summer steelhead; 878 adult and 43 jack fall Chinook; and 560 adult and 28 jack spring Chinook were collected as broodstock for the Umatilla River hatchery program. In addition, there were 241 adult and 15 jack spring Chinook collected at Threemile Dam for outplanting in the South Fork Walla Walla River and Mill Cr, a tributary of the mainstem Walla Walla River. The Westland Canal juvenile facility (Westland), located near the town of Echo at river mile (RM) 27, is the major collection point for out-migrating juvenile salmonids and steelhead kelts. The canal was open for 158 days between February 11, 2008 and July 18, 2008. During that period, fish were bypassed back to the river 150 days and were trapped 6 days. There were also 2 days when fish were directed into and held in the canal forebay between the time the bypass was closed and the trap opened. An estimated 64 pounds of fish were transported from the Westland trapping facility. Approximately 25.8% of the fish transported were salmonids. In addition, one adult Pacific lamprey was trapped and released above the Westland ladder this year. The Threemile Dam west bank juvenile bypass was opened on March 11, 2008 in conjunction with water deliveries and continued through the summer. West Extension Irrigation District (WEID) discontinued diverting live flow on June 24, 2008 but the bypass remained open throughout the project year. The juvenile trap was not operated this project year.« less

Characteristics of Physical Activity Programs for Older Adults: Results of a Multisite Survey

ERIC Educational Resources Information Center

Hughes, Susan L.; Williams, Barbara; Molina, Lourdes C.; Bayles, Constance; Bryant, Lucinda L.; Harris, Jeffrey R.; Hunter, Rebecca; Ivey, Susan; Watkins, Ken

2005-01-01

Purpose: Although increased participation in physical activity by older adults is a major public health goal, little is known about the supply and use of physical activity programs in the United States. Design and Methods: Seven academic centers in diverse geographic areas surveyed physical activity programs for older adults. Five sites conducted…

NASA's Hall Thruster Program 2002

NASA Technical Reports Server (NTRS)

Jankovsky, Robert S.; Jacobson, David T.; Pinero, Luis R.; Manzella, David H.; Hofer, Richard R.; Peterson, Peter Y.

2002-01-01

The NASA Hall thruster program currently supports a number of tasks related to high power thruster development for a number of customers including the Energetics Program (formerly called the Space-based Program), the Space Solar Power Program, and the In-space Propulsion Program. In program year 2002, two tasks were central to the NASA Hall thruster program: 1) the development of a laboratory Hall thruster capable of providing high thrust at high power-, and 2) investigations into operation of Hall thrusters at high specific impulse. In addition to these two primary thruster development activities, there are a number of other on-going activities supported by the NASA Hall thruster program. These additional activities are related to issues such as high-power power processor architecture, thruster lifetime, and spacecraft integration.

An internet survey of the characteristics and physical activity of community-dwelling Australian adults with acquired brain injury: Exploring interest in an internet-delivered self-management program focused on physical activity.

PubMed

Jones, Taryn M; Dean, Catherine M; Dear, Blake F; Hush, Julia M; Titov, Nickolai

2016-01-01

Individuals with acquired brain injury (ABI) are more likely to be physically inactive and experience barriers to accessing services to address inactivity. This study was designed to guide the development of an internet-delivered self-management program to increase physical activity after ABI. The aims of this study were to examine the current physical activity status of community-dwelling Australian adults with ABI, the barriers to physical activity they experience and to explore interest an internet-delivered self-management program aimed at increasing physical activity. An online survey of Australian adults with ABI was used to collect information about demographic characteristics; general health; emotional well-being; mobility and physical activity status, and satisfaction; barriers to physical activity; confidence in overcoming barriers, and; interest in an internet self-management program. Data were analyzed descriptively and correlational analyses examined relationships between variables. Data were analyzed from 59 respondents. Over half were not satisfied with their current physical activity status. The most frequently reported barriers were pain/discomfort, fatigue and fear, and confidence to overcome these barriers was very low. Interest in an internet-delivered self-management program was high (74%) and not related to the amount of physical activity, satisfaction with physical activity and mobility status or total number of barriers. Australian adults with ABI are not satisfied with their activity levels and experience barriers in maintaining their physical activity levels. Participants were interested in accessing an internet-delivered self-management program aimed at improving physical activity levels. Therefore such a program warrants development and evaluation. Copyright © 2016 Elsevier Inc. All rights reserved.

22 CFR 216.2 - Applicability of procedures.

Code of Federal Regulations, 2010 CFR

2010-04-01

... river basin development; (ii) Irrigation or water management projects, including dams and impoundments... projects, programs or activities authorized or approved by A.I.D. and to substantive amendments or extensions of ongoing projects, programs, or activities. (b) Exemptions. (1) Projects, programs or activities...

29 CFR 32.4 - Discrimination prohibited.

Code of Federal Regulations, 2010 CFR

2010-07-01

... the objectives of the recipient's program or activity with respect to handicapped individuals; or (iii... discrimination under any program or activity that receives Federal financial assistance; or (ii) That have the..., service or training provided under a program or activity receiving Federal financial assistance includes...

Annual progress report : for the period of January 2001 through December 2001 : Florida Transit Training Program (2001) : Florida Technical Assistance Program (2001)

DOT National Transportation Integrated Search

2001-01-01

The following progress report is intended to highlight the significant activities of the Florida Transit Training Program and Florida Technical Assistant Program for the 2001 year. Activities of the Florida Statewide Transit Training Program are pres...

Overview of the Novel Intelligent JAXA Active Rotor Program

NASA Technical Reports Server (NTRS)

Saito, Shigeru; Kobiki, Noboru; Tanabe, Yasutada; Johnson, Wayne; Yamauchi, Gloria K.; Young, Larry A.

2010-01-01

The Novel Intelligent JAXA Active Rotor (NINJA Rotor) program is a cooperative effort between JAXA and NASA, involving a test of a JAXA pressure-instrumented, active-flap rotor in the 40- by 80-Foot Wind Tunnel at Ames Research Center. The objectives of the program are to obtain an experimental database of a rotor with active flaps and blade pressure instrumentation, and to use that data to develop analyses to predict the aerodynamic and aeroacoustic performance of rotors with active flaps. An overview of the program is presented, including a description of the rotor and preliminary pretest calculations.

A randomized controlled trial of an activity specific exercise program for individuals with Alzheimer disease in long-term care settings.

PubMed

Roach, Kathryn E; Tappen, Ruth M; Kirk-Sanchez, Neva; Williams, Christine L; Loewenstein, David

2011-01-01

To determine whether an activity specific exercise program could improve ability to perform basic mobility activities in long-term care residents with Alzheimer disease (AD). Randomized, controlled, single-blinded clinical trial. Residents of 7 long-term care facilities. Eighty-two long-term care residents with mild to severe AD. An activity specific exercise program was compared to a walking program and to an attention control. Ability to perform bed mobility and transfers was assessed using the subscales of the Acute Care Index of Function; functional mobility was measured using the 6-Minute Walk test. Subjects receiving the activity specific exercise program improved in ability to perform transfers, whereas subjects in the other 2 groups declined.

45 CFR 287.130 - Can NEW Program activities include job market assessments, job creation and economic development...

Code of Federal Regulations, 2011 CFR

2011-10-01

... Program Design and Operations § 287.130 Can NEW Program activities include job market assessments, job...-sufficiency of program participants; (6) Surveys to collect information regarding client characteristics; and...

45 CFR 287.130 - Can NEW Program activities include job market assessments, job creation and economic development...

Code of Federal Regulations, 2014 CFR

2014-10-01

... Program Design and Operations § 287.130 Can NEW Program activities include job market assessments, job...-sufficiency of program participants; (6) Surveys to collect information regarding client characteristics; and...

Active vibration control testing of the SPICES program: final demonstration article

NASA Astrophysics Data System (ADS)

Dunne, James P.; Jacobs, Jack H.

1996-05-01

The Synthesis and Processing of Intelligent Cost Effective Structures (SPICES) Program is a partnership program sponsored by the Advanced Research Projects Agency. The mission of the program is to develop cost effective material processing and synthesis technologies to enable new products employing active vibration suppression and control devices to be brought to market. The two year program came to fruition in 1995 through the fabrication of the final smart components and testing of an active plate combined with two trapezoidal rails, forming an active mount. Testing of the SPICES combined active mount took place at McDonnell Douglas facilities in St. Louis, MO, in October-December 1995. Approximately 15 dB reduction in overall response of a motor mounted on the active structure was achieved. Further details and results of the SPICES combined active mount demonstration testing are outlined. Results of numerous damping and control strategies that were developed and employed in the testing are presented, as well as aspects of the design and fabrication of the SPICES active mount components.

7 CFR 15a.4 - Assurance required.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Office of the Secretary of Agriculture EDUCATION PROGRAMS OR ACTIVITIES RECEIVING OR BENEFITTING FROM... Federal financial assistance for any education program or activity shall as condition of its approval..., that each education program or activity operated by the applicant or recipient and to which this part...

38 CFR 18.404 - Discrimination prohibited.

Code of Federal Regulations, 2010 CFR

2010-07-01

... subjected to discrimination under any program or activity which receives Federal financial assistance. (b... accomplishment of the objectives of the recipient's program or activity with respect to handicapped persons, or... under any program or activity that receives Federal financial assistance, or (ii) Have the purpose or...

24 CFR 6.4 - Discrimination prohibited.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Development NONDISCRIMINATION IN PROGRAMS AND ACTIVITIES RECEIVING ASSISTANCE UNDER TITLE I OF THE HOUSING AND..., for programs or activities receiving Federal financial assistance, the regulations in this part 6... benefits of, or be subjected to discrimination under any program or activity funded in whole or in part...

A diabetes self-management program designed for urban American Indians.

PubMed

Castro, Sarah; O'Toole, Mary; Brownson, Carol; Plessel, Kimberly; Schauben, Laura

2009-10-01

Although the American Indian population has a disproportionately high rate of type 2 diabetes, little has been written about culturally sensitive self-management programs in this population. Community and clinic partners worked together to identify barriers to diabetes self-management and to provide activities and services as part of a holistic approach to diabetes self-management, called the Full Circle Diabetes Program. The program activities and services addressed 4 components of holistic health: body, spirit, mind, and emotion. Seven types of activities or services were available to help participants improve diabetes self-management; these included exercise classes, educational classes, and talking circles. Ninety-eight percent of program enrollees participated in at least 1 activity, and two-thirds participated in 2 or more activities. Program participation resulted in a significant improvement in knowledge of resources for managing diabetes. The Full Circle Diabetes Program developed and implemented culturally relevant resources and supports for diabetes self-management in an American Indian population. Lessons learned included that a holistic approach to diabetes self-management, community participation, and stakeholder partnerships are needed for a successful program.

Promoting physical activity among youth through community-based prevention marketing.

PubMed

Bryant, Carol A; Courtney, Anita H; McDermott, Robert J; Alfonso, Moya L; Baldwin, Julie A; Nickelson, Jen; McCormack Brown, Kelli R; Debate, Rita D; Phillips, Leah M; Thompson, Zachary; Zhu, Yiliang

2010-05-01

Community-based prevention marketing (CBPM) is a program planning framework that blends community-organizing principles with a social marketing mind-set to design, implement, and evaluate public health interventions. A community coalition used CBPM to create a physical activity promotion program for tweens (youth 9-13 years of age) called VERB Summer Scorecard. Based on the national VERB media campaign, the program offered opportunities for tweens to try new types of physical activity during the summer months. The VERB Summer Scorecard was implemented and monitored between 2004 and 2007 using the 9-step CBPM framework. Program performance was assessed through in-depth interviews and a school-based survey of youth. The CBPM process and principles used by school and community personnel to promote physical activity among tweens are presented. Observed declines may become less steep if school officials adopt a marketing mind-set to encourage youth physical activity: deemphasizing health benefits but promoting activity as something fun that fosters spending time with friends while trying and mastering new skills. Community-based programs can augment and provide continuity to school-based prevention programs to increase physical activity among tweens.

Differences in psychosocial determinants of physical activity in older adults participating in organised versus non-organised activities.

PubMed

Deforche, B; De Bourdeaudhuij, I

2000-12-01

With the introduction of the new consensus on 30 minutes of moderate physical activity preferably on all days of the week, exercise implemented into daily activities is promoted whereas structured activity programs lose importance. Activity levels of most older people don't come up with current recommendations. Therefore strategies to enhance attendance of older adults in physical activities should be developed. Group programs may be more effective in changing exercise behaviour of older adults than non-supervised physical activity. The purpose of this study was to investigate the differences in level of activity and psychosocial determinants of physical activity between seniors involved in an exercise class and seniors not engaged in any organised physical activity. Seventy-five elderly who were currently involved in structured exercise classes and 75 elderly who did not participate in any organised physical activity during the previous year were recruited in senior citizens' centres and were asked to fill out a questionnaire. Subjects involved in an exercise program had higher levels of activity and reported more social influences and higher self-efficacy compared to the respondents practising on an individual basis. No differences were found in perceived barriers or benefits. Exercising in a group program gives the opportunity to accumulate some extra physical activity and positively affects the level of activity outside the program. Stimulating older adults to join a structured activity program in the company of family or friends in order to enhance supporting social influences and perceived competence could be an important intervention strategy.

Eat better & move more: a community-based program designed to improve diets and increase physical activity among older Americans.

PubMed

Wellman, Nancy S; Kamp, Barbara; Kirk-Sanchez, Neva J; Johnson, Paulette M

2007-04-01

We assessed outcomes of an integrated nutrition and exercise program designed for Older Americans Act Nutrition Program participants as part of the Administration on Aging's You Can! campaign. A 10-site intervention study was conducted. Preintervention and postintervention assessments focused on nutrition and physical activity stages of change, self-reported health status, dietary intakes, physical activity, and program satisfaction. Of 999 enrollees, the 620 who completed the program were aged 74.6 years on average; 82% were women, and 41% were members of racial/ethnic minority groups. Factors associated with program completion were site, health conditions, and nutrition risk. Seventy-three percent and 75% of participants, respectively, made a significant advance of 1 or more nutrition and physical activity stages of change; 24% reported improved health status. Daily intake of fruit increased 1 or more servings among 31% of participants; vegetables, 37%; and fiber, 33%. Daily steps increased 35%; blocks walked, 45%; and stairs climbed, 24%. Program satisfaction was 99%. This easy-to-implement program improves diets and activity levels. Local providers should offer more such programs with the goal of enabling older Americans to take simple steps toward successful aging.

Embryonic and larval development in barfin flounder Verasper moseri (Jordan and Gilbert)

NASA Astrophysics Data System (ADS)

Du, Rongbin; Wang, Yongqiang; Jiang, Haibin; Liu, Liming; Wang, Maojian; Li, Tianbao; Zhang, Shubao

2010-01-01

Broodstock of Verasper moseri (Jordan and Gilbert) aged 3-4 years old were selected, and reinforced cultivation was conducted to promote maturation under controlled water temperature and photoperiod conditions. Fertilized eggs were obtained by artificial fertilization, and the development of embryos, larvae and juveniles was observed continuously. The results showed that the fertilized eggs of V. moseri were spherical, with transparent yolk and homogeneous bioplasm, and had no oil globule inside. The average diameter of the eggs was 1.77±0.02 mm. The eggs of V. moseri were buoyant in water with salinity above 35. The cleavage type was typical discoidal. Young pigment cells appeared when olfactory plates began to form. Hatching occurred at 187 h after fertilization at a water temperature of 8.5°C. The newly hatched larvae, floating on the water surface, were transparent with an average total length of 4.69±0.15 mm. During the cultivation period, when the water temperature was raised from 9 to 14.5°C, 4-day old larvae showed more melanophores on the body surface, making the larvae gray in color. The pectoral fins began to develop, which enabled the larvae to swim horizontally and in a lively manner. On days 7-8, the digestive duct formed. The yolk sac was small and black. The yolk sac was absorbed on day 11. Larvae took food actively, and body length and body height clearly increased. The rudiments of dorsal and anal fin pterygiophores were discernible and caudal fin ray elements formed on day 19. On day 24, the larval notochord flexed upwards, and the rays of unpaired fins began to differentiate. Pigment cells converged on the dorsal and anal fin rays, and the mastoid teeth on the mandible appeared. On day 29, the left eyes of juveniles began to move upwards. Depigmentation began in some juveniles and they became sandy brown in color on day 37. Most juveniles began to settle on the bottom of the tank. The left eyes of juveniles migrated completely to the right side on day 50, when the average body length attained 2.5±0.18 cm, and juveniles accomplished metamorphosis to young. The embryonic and larval characters of several flounder species are compared.

Mobility, strength, and fitness after a graded activity program for patients with subacute low back pain. A randomized prospective clinical study with a behavioral therapy approach.

PubMed

Lindström, I; Ohlund, C; Eek, C; Wallin, L; Peterson, L E; Nachemson, A

1992-06-01

Patients with nonspecific mechanical low back pain (n = 103), examined by an orthopaedic surgeon and a social worker, were randomized to an activity group (n = 51) and a control group (n = 52). Patients with defined orthopaedic, medical, or psychiatric diagnoses were excluded before randomization. No patients were excluded due to place of birth or difficulties in speaking or understanding the Swedish language. The purpose of the study was to compare mobility, strength and fitness after traditional care and after traditional care plus a graded activity program with a behavioral therapy approach. A graded activity program, with a behavioral therapy approach was given under the guidance of a physical therapist. The endpoint of the graded activity program was return to work. This program significantly increased mobility, strength, and fitness more than could be explained by only a time recovery effect, especially in males. The patients in the activity group returned to work earlier than did the patients in the control group. Spinal rotation, abdominal muscle endurance time and lifting capacity were significantly correlated to rate of return to work. Traditional care plus a graded activity program were superior to only traditional care, evaluated in terms of mobility, strength and fitness. The graded activity program proved to be a successful method of restoring occupational function and facilitating return to work in subacute low back pain patients. The patients in the graded activity program learned that it is safe to move, while regaining function.

Adherence to a Videogame-Based Physical Activity Program for Older Adults with Schizophrenia

PubMed Central

Hubbard, Erin M.; Dowling, Glenna A.

2014-01-01

Abstract Objectives: Adults with schizophrenia are a growing segment of the older adult population. Evidence suggests that they engage in limited physical activity. Interventions are needed that are tailored around their unique limitations. An active videogame-based physical activity program that can be offered at a treatment facility can overcome these barriers and increase motivation to engage in physical activity. The purpose of this report is to describe the adherence to a videogame-based physical activity program using the Kinect® for Xbox® 360 game system (Microsoft®, Redmond, WA) in older adults with schizophrenia. Materials and Methods: This was a descriptive longitudinal study among 34 older adults with schizophrenia to establish the adherence to an active videogame-based physical activity program. In our ongoing program, once a week for 6 weeks, participants played an active videogame, using the Kinect for Xbox 360 game system, for 30 minutes. Adherence was measured with a count of sessions attended and with the total minutes attended out of the possible total minutes of attendance (180 minutes). Results: Thirty-four adults with schizophrenia enrolled in the study. The mean number of groups attended was five out of six total (standard deviation=2), and the mean total minutes attended were 139 out of 180 possible (standard deviation=55). Fifty percent had perfect attendance. Conclusions: Older adults with schizophrenia need effective physical activity programs. Adherence to our program suggests that videogames that use the Kinect for Xbox 360 game system are an innovative way to make physical activity accessible to this population. PMID:26192371

Promoting Optimal Physical Exercise for Life: An Exercise and Self-Management Program to Encourage Participation in Physical Activity after Discharge from Stroke Rehabilitation—A Feasibility Study

PubMed Central

Mansfield, Avril; Knorr, Svetlana; Poon, Vivien; Inness, Elizabeth L.; Middleton, Laura; Biasin, Louis; Brunton, Karen; Howe, Jo-Anne; Brooks, Dina

2016-01-01

People with stroke do not achieve adequate levels of physical exercise following discharge from rehabilitation. We developed a group exercise and self-management program (PROPEL), delivered during stroke rehabilitation, to promote uptake of physical activity after discharge. This study aimed to establish the feasibility of a larger study to evaluate the effect of this program on participation in self-directed physical activity. Participants with subacute stroke were recruited at discharge from one of three rehabilitation hospitals; one hospital offered the PROPEL program whereas the other two did not (comparison group; COMP). A high proportion (11/16) of eligible PROPEL program participants consented to the study. Fifteen COMP participants were also recruited. Compliance with wearing an accelerometer for 6 weeks continuously and completing physical activity questionnaires was high (>80%), whereas only 34% of daily heart rate data were available. Individuals who completed the PROPEL program seemed to have higher outcome expectations for exercise, fewer barriers to physical activity, and higher participation in physical activity than COMP participants (Hedge's g ≥ 0.5). The PROPEL program delivered during stroke rehabilitation shows promise for reducing barriers to exercise and increasing participation in physical activity after discharge. This study supports feasibility of a larger randomized trial to evaluate this program. PMID:27313948

24 CFR 3.415 - Access to course offerings.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 3... any of its education program or activity separately on the basis of sex, or require or refuse...

32 CFR 196.415 - Access to course offerings.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 196... any of its education program or activity separately on the basis of sex, or require or refuse...

24 CFR 3.415 - Access to course offerings.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 3... any of its education program or activity separately on the basis of sex, or require or refuse...

32 CFR 196.415 - Access to course offerings.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 196... any of its education program or activity separately on the basis of sex, or require or refuse...

38 CFR 23.415 - Access to course offerings.

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 23... any of its education program or activity separately on the basis of sex, or require or refuse...

32 CFR 196.415 - Access to course offerings.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 196... any of its education program or activity separately on the basis of sex, or require or refuse...

45 CFR 2555.415 - Access to course offerings.

Code of Federal Regulations, 2011 CFR

2011-10-01

... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities... carry out any of its education program or activity separately on the basis of sex, or require or refuse...

24 CFR 3.415 - Access to course offerings.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 3... any of its education program or activity separately on the basis of sex, or require or refuse...

45 CFR 2555.415 - Access to course offerings.

Code of Federal Regulations, 2012 CFR

2012-10-01

... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities... carry out any of its education program or activity separately on the basis of sex, or require or refuse...

24 CFR 3.415 - Access to course offerings.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 3... any of its education program or activity separately on the basis of sex, or require or refuse...

45 CFR 2555.415 - Access to course offerings.

Code of Federal Regulations, 2013 CFR

2013-10-01

... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities... carry out any of its education program or activity separately on the basis of sex, or require or refuse...

38 CFR 23.415 - Access to course offerings.

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 23... any of its education program or activity separately on the basis of sex, or require or refuse...

45 CFR 2555.415 - Access to course offerings.

Code of Federal Regulations, 2014 CFR

2014-10-01

... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities... carry out any of its education program or activity separately on the basis of sex, or require or refuse...

38 CFR 23.415 - Access to course offerings.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 23... any of its education program or activity separately on the basis of sex, or require or refuse...

32 CFR 196.415 - Access to course offerings.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 196... any of its education program or activity separately on the basis of sex, or require or refuse...

38 CFR 23.415 - Access to course offerings.

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 23... any of its education program or activity separately on the basis of sex, or require or refuse...

75 FR 9155 - Notice of Funds Availability (NOFA) Inviting Applications for the 2010 Farmers' Market Promotion...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-01

... farmers' markets, roadside stands, community supported agriculture programs, agri-tourism activities, and...-supported agriculture programs, agri-tourism activities and other direct producer-to-consumer market..., community-supported agriculture programs, agri-tourism activities, and other direct producer-to-consumer...

78 FR 57641 - Proposed Information Collection Activity; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-19

..., designed to improve the well-being of children affected by parental substance abuse. On September 28, 2012... the programs and activities conducted through the RPG Grant Program. Title: RPG National Cross-Site... integration of programs, activities, and services designed to increase well-being, improve permanency, and...

7 CFR 15b.4 - Discrimination prohibited.

Code of Federal Regulations, 2010 CFR

2010-01-01

... impairing accomplishment of the objectives of the recipient's program or activity with respect to... subjecting them to discrimination under any program or activity that receives Federal financial assistance or... objectives of the program or activity with respect to handicapped persons. (6) As used in this section, an...

75 FR 66699 - Farm Loan Programs Loan Making Activities; Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-29

... Loan Programs Loan Making Activities; Correction AGENCY: Farm Service Agency, USDA. ACTION: Proposed rule; correction. SUMMARY: This document contains a correction to the proposed rule titled ``Farm Loan Programs Loan Making Activities'' that was published September 23, 2010. The Farm Service Agency (FSA) is...

Facts about Physical Activity

MedlinePlus

... Physical Activity Overweight & Obesity Healthy Weight Breastfeeding Micronutrient Malnutrition State and Local Programs Facts about Physical Activity ... Physical Activity Overweight & Obesity Healthy Weight Breastfeeding Micronutrient Malnutrition State and Local Programs File Formats Help: How ...

The impact of an m-Health financial incentives program on the physical activity and diet of Australian truck drivers.

PubMed

Gilson, Nicholas D; Pavey, Toby G; Wright, Olivia Rl; Vandelanotte, Corneel; Duncan, Mitch J; Gomersall, Sjaan; Trost, Stewart G; Brown, Wendy J

2017-05-18

Chronic diseases are high in truck drivers and have been linked to work routines that promote inactivity and poor diets. This feasibility study examined the extent to which an m-Health financial incentives program facilitated physical activity and healthy dietary choices in Australian truck drivers. Nineteen men (mean [SD] age = 47.5 [9.8] years; BMI = 31.2 [4.6] kg/m 2 ) completed the 20-week program, and used an activity tracker and smartphone application (Jawbone UP™) to regulate small positive changes in occupational physical activity, and fruit, vegetable, saturated fat and processed/refined sugar food/beverage choices. Measures (baseline, end-program, 2-months follow-up; April-December 2014) were accelerometer-determined proportions of work time spent physically active, and a workday dietary questionnaire. Statistical (repeated measures ANOVA) and thematic (interviews) analyses assessed program impact. Non-significant increases in the mean proportions of work time spent physically active were found at end-program and follow-up (+1%; 7 mins/day). Fruit (p = 0.023) and vegetable (p = 0.024) consumption significantly increased by one serve/day at end-program. Non-significant improvements in saturated fat (5%) and processed/refined sugar (1%) food/beverage choices were found at end-program and follow-up. Overall, 65% (n = 11) of drivers demonstrated positive changes in physical activity, and at least one dietary choice (e.g. saturated fat) at follow-up. Drivers found the financial incentives component of the program to be a less effective facilitator of change than the activity tracker and smartphone application, although this technology was easier to use for monitoring of physical activity than healthy dietary choices. Not all drivers benefitted from the program. However, positive changes for different health behaviours were observed in the majority of participants. Outcomes from this feasibility study inform future intervention development for studies with larger samples. ANZCTR12616001513404 . Registered November 2nd, 2016 (retrospectively registered).

Project Planning and Reporting

NASA Technical Reports Server (NTRS)

1982-01-01

Project Planning Analysis and Reporting System (PPARS) is automated aid in monitoring and scheduling of activities within project. PPARS system consists of PPARS Batch Program, five preprocessor programs, and two post-processor programs. PPARS Batch program is full CPM (Critical Path Method) scheduling program with resource capabilities. Can process networks with up to 10,000 activities.

75 FR 20541 - Homeless Emergency Assistance and Rapid Transition to Housing: Defining “Homeless”

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-20

... Housing Stability Program. The HEARTH Act also codifies in statutory law the Continuum of Care planning... activities and to add rapid re-housing activities. The new Rural Housing Stability program replaces the Rural... Care program, and the Rural Housing Stability program. Each of these programs will include the...

Challenges to Evaluating Physical Activity Programs in American Indian/Alaska Native Communities

ERIC Educational Resources Information Center

Roberts, Erica Blue; Butler, James; Green, Kerry M.

2018-01-01

Despite the importance of evaluation to successful programming, a lack of physical activity program (PAP) evaluation for American Indian/Alaska Native (AI/AN) programs exists, which is significant given the high rates of obesity and diabetes in this population. While evaluation barriers have been identified broadly among AI/AN programs, challenges…

Faculty development activities in family medicine: in search of innovation.

PubMed

Lawrence, Elizabeth A; Oyama, Oliver N

2013-01-01

To describe the Accreditation Council for Graduate Medical Education's (ACGME) faculty development requirements, explore the range of faculty development activities and support currently used by family medicine residencies to meet these requirements, and describe one innovative approach to satisfy this need. An electronic survey of faculty development activities and support offered to faculty by residency programs was sent to a random sample of 40 medical school and community based family medicine residency programs across the United States. Data were examined using t-tests, Fisher's exact tests, and Analysis of Variance. Faculty development, beyond traditional clinical CME, was strongly encouraged or required by a large proportion of the sample (73%). Only 58% of programs reported having discussed the ACGME's faculty development component areas (clinical, educational, administrative, leadership, research, and behavioral). In each component area except the "clinical" area, the absence of discussing the ACGME component areas with residency faculty was associated with fewer faculty development activities and support being offered by the program. These results, although preliminary, suggest that family medicine residency programs may value and encourage faculty development. The majority of programs use traditional activities and strategies such as CME, faculty meetings, faculty conferences and workshops; and a smaller number of programs are exploring the utility of mentoring programs, faculty discussion groups, and technology based learning systems. The challenge is to develop faculty development activities tailored to individual program and faculty needs and resources.

Structural conceptualization of learning experiences in nursing master's degree programs utilized in occupational health nursing activities.

PubMed

Aoyama, Wakako; Tatsumi, Asami

2017-01-31

In this study, concepts were constructed that express learning experiences in nursing master's degree programs utilized in occupational health nursing activities with the aim of clarifying those characteristics. This was based on the idea that elucidation of the characteristics of learning experiences in nursing master's degree programs used in occupational health nursing activities would be meaningful in providing high-quality occupational health services that respond to the needs of society. Semi-structured interviews were conducted with 10 people who fulfilled the three conditions of having completed a master's degree programs, working as an occupational health nurse after completion of the program, and not continuing on to a doctoral program. The nursing conceptualization method of Naomi Funashima was used. From the obtained data, 512 code items expressing learning experiences in master's degree programs utilized in occupational health nursing activities were identified. These items included five core categories (concepts), 34 categories, and 69 subcategories. The five concepts constructed were "Pursuit of expertise and self-evaluation," "Mutual understanding of various people that leads to human resources utilization," "Theoretical and academic learning that influences changes in activities," "Research learning that lead to activities based on scientific evidence," and "Learning that leads to high-quality activities making use of expertise." It was found that various learning experiences in the master's program to pursue the specialty of occupational health nurses in order to recognize their roles as well as the experiences to take the initiative in learning had been integrated in their activities after completion of the course and had contributed to their high-quality occupational health nursing activities. It was suggested that the learning experiences in the master's program, which had been revealed in this study, were the experiences necessary for providing high-quality occupational health nursing activities to satisfy the social needs.

Remote sensing education in NASA's technology transfer program

NASA Technical Reports Server (NTRS)

Weinstein, R. H.

1981-01-01

Remote sensing is a principal focus of NASA's technology transfer program activity with major attention to remote sensing education the Regional Program and the University Applications Program. Relevant activities over the past five years are reviewed and perspective on future directions is presented.

Ethical Development through Student Activities Programming.

ERIC Educational Resources Information Center

Brock, Carol S.

1991-01-01

Student activities programing, viewed as essential to the college experience, is defended by outlining some of the values and growth opportunities it provides for students. Several specific programing strategies useful as catalysts in values development are described, including values clarification exercises, multicultural programing, and…

34 CFR 106.31 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 1 2011-07-01 2011-07-01 false Education programs or activities. 106.31 Section 106.31 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL...

49 CFR 37.61 - Public transportation programs and activities in existing facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Public transportation programs and activities in... TRANSPORTATION SERVICES FOR INDIVIDUALS WITH DISABILITIES (ADA) Transportation Facilities § 37.61 Public transportation programs and activities in existing facilities. (a) A public entity shall operate a designated...

34 CFR 647.4 - What activities and services may a project provide?

Code of Federal Regulations, 2010 CFR

2010-07-01

... PROGRAM General § 647.4 What activities and services may a project provide? A McNair project may provide... activities designed to prepare participants for doctoral study. (d) Tutoring. (e) Academic counseling. (f... programs. (g) Mentoring programs involving faculty members or students at institutions of higher education...

12 CFR 268.704 - General prohibitions against discrimination.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Programs and Activities Because of Physical or Mental Disability § 268.704 General prohibitions against... program or activity conducted by the Board. (b)(1) The Board, in providing any aid, benefit, or service... opportunity to participate in programs or activities that are not separate or different, despite the existence...

12 CFR 268.704 - General prohibitions against discrimination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Programs and Activities Because of Physical or Mental Disability § 268.704 General prohibitions against... program or activity conducted by the Board. (b)(1) The Board, in providing any aid, benefit, or service... opportunity to participate in programs or activities that are not separate or different, despite the existence...

77 FR 37687 - Assistance to Firefighters Grant Program

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-22

... importance of mitigation activities, the FY 2012 FP&S will be allocated $35 million for grants. The FP&S... grants supporting eligible fire prevention activities and research and development activities that...,250) of AFG Program funds for the FP&S Grant Program. However, due to the importance of mitigation...

49 CFR 25.400 - Education programs or activities.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 25.400 Transportation Office of the Secretary of Transportation NONDISCRIMINATION ON THE BASIS OF SEX... Basis of Sex in Education Programs or Activities Prohibited § 25.400 Education programs or activities... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected to...

36 CFR 1211.415 - Access to course offerings.

Code of Federal Regulations, 2014 CFR

2014-07-01

... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities... carry out any of its education program or activity separately on the basis of sex, or require or refuse...

36 CFR 1211.415 - Access to course offerings.

Code of Federal Regulations, 2012 CFR

2012-07-01

... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities... carry out any of its education program or activity separately on the basis of sex, or require or refuse...

36 CFR 1211.415 - Access to course offerings.

Code of Federal Regulations, 2011 CFR

2011-07-01

... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities... carry out any of its education program or activity separately on the basis of sex, or require or refuse...

36 CFR § 1211.415 - Access to course offerings.

Code of Federal Regulations, 2013 CFR

2013-07-01

... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities... carry out any of its education program or activity separately on the basis of sex, or require or refuse...

Case Study of an Institutionalized Urban Comprehensive School Physical Activity Program

ERIC Educational Resources Information Center

Doolittle, Sarah A.; Rukavina, Paul B.

2014-01-01

This single case study (Yin, 2009) compares an established urban physical education/ sport/physical activity program with two models: Comprehensive School Physical Activity Program/CSPAP (AAHPERD, 2013; CDC, 2013); and Lawson's propositions (2005) for sport, exercise and physical education for empowerment and community development to determine…

34 CFR 106.31 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 1 2010-07-01 2010-07-01 false Education programs or activities. 106.31 Section 106.31 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL...

76 FR 54772 - Notice of Intent To Award Affordable Care Act Funding, Funding Opportunity Announcement CDC-RFA...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-02

... applications under the Nutrition, Physical Activity, and Obesity Program. These applications have been..., Physical Activity, and Obesity Program Funding Opportunity Announcement (FOA). Award Information... based on methodology published in the Nutrition, Physical Activity, and Obesity Program CDC-RFA-DP08-805...

Participant Perceptions of an Individualised Physical Activity Anti-Smoking Intervention

ERIC Educational Resources Information Center

Hanlon, Clare; Morris, Tony; O'Sullivan, Grant Anthony

2018-01-01

Purpose: The purpose of this paper is to explore a health program comprising the individual experiences, successes and setbacks of adults in an individually tailored, community-based smoking intervention and physical activity program. The program incorporated physical activity consultation (PAC) and phone support from the well-established Quit…

76 FR 69204 - Anti-Money Laundering Program and Suspicious Activity Reporting Requirements for Housing...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-08

... 1506-AB14 Anti-Money Laundering Program and Suspicious Activity Reporting Requirements for Housing... enterprises as financial institutions for the purpose of requiring them to establish anti-money laundering... organizations to establish anti-money laundering programs and report suspicious activities is intended to help...

The effects of a client-centered leisure activity program on satisfaction, self-esteem, and depression in elderly residents of a long-term care facility

PubMed Central

Jung, Ji-Yoon; Park, So-Yeon; Kim, Jin-Kyung

2018-01-01

[Purpose] This study aimed to examine the effects of a client-centered leisure activity program on satisfaction, upper limb function, self-esteem, and depression in elderly residents of a long-term care facility. [Subjects and Methods] This study included 12 elderly subjects, aged 65 or older, residing in a nursing home. The subjects were divided into an experimental and a control group. Subjects in the control group received leisure activities already provided by the facility. The experimental group participated in a client-centered leisure activity program. The subjects conducted individual activities three times per week, 30 minutes per session. The group activity was conducted three times per week for eight weeks. Each subject’s performance of and satisfaction with the leisure activity programs, upper limb function, self-esteem, and depression were measured before and after the intervention. [Results] After participating in a program, significant improvements were seen in both the Canadian Occupational Performance Measure and upper limb function in the experimental group. Also after the intervention, the subjects’ self-esteem significantly increased and their depression significantly decreased. [Conclusion] A client-centered leisure activity program motivates elderly people residing in a long-term care facility and induces their voluntary participation. Such customized programs are therefore effective for enhancing physical and psychological functioning in this population. PMID:29410570

Results of the First Year of Active for Life: Translation of 2 Evidence-Based Physical Activity Programs for Older Adults Into Community Settings

PubMed Central

Wilcox, Sara; Dowda, Marsha; Griffin, Sarah F.; Rheaume, Carol; Ory, Marcia G.; Leviton, Laura; King, Abby C.; Dunn, Andrea; Buchner, David M.; Bazzarre, Terry; Estabrooks, Paul A.; Campbell-Voytal, Kimberly; Bartlett-Prescott, Jenny; Dowdy, Diane; Castro, Cynthia M.; Carpenter, Ruth Ann; Dzewaltowski, David A.; Mockenhaupt, Robin

2006-01-01

Objectives. Translating efficacious interventions into practice within community settings is a major public health challenge. We evaluated the effects of 2 evidence-based physical activity interventions on self-reported physical activity and related outcomes in midlife and older adults. Methods. Four community-based organizations implemented Active Choices, a 6-month, telephone-based program, and 5 implemented Active Living Every Day, a 20-week, group-based program. Both programs emphasize behavioral skills necessary to become more physically active. Participants completed pretest and posttest surveys. Results. Participants (n=838) were aged an average of 68.4 ±9.4 years, 80.6% were women, and 64.1% were non-Hispanic White. Seventy-two percent returned posttest surveys. Intent-to-treat analyses found statistically significant increases in moderate-to-vigorous physical activity and total physical activity, decreases in depressive symptoms and stress, increases in satisfaction with body appearance and function, and decreases in body mass index. Conclusions. The first year of Active for Life demonstrated that Active Choices and Active Living Every Day, 2 evidence-based physical activity programs, can be successfully translated into community settings with diverse populations. Further, the magnitudes of change in outcomes were similar to those reported in the efficacy trials. PMID:16735619

Community Agency Survey Formative Research Results From the TAAG Study

PubMed Central

Saunders, Ruth P.; Moody, Jamie

2008-01-01

School and community agency collaboration can potentially increase physical activity opportunities for youth. Few studies have examined the role of community agencies in promoting physical activity, much less in collaboration with schools. This article describes formative research data collection from community agencies to inform the development of the Trial of Activity for Adolescent Girls (TAAG) intervention to provide out-of-school physical activity programs for girls. The community agency survey is designed to assess agency capacity to provide physical activity programs for girls, including resources, programs, and partnerships. Most agency respondents (n = 138) report operations during after-school hours, adequate facilities, and program options for girls, although most are sport oriented. Agency resources and programming vary considerably across the six TAAG field sites. Many agencies report partnerships, some involving schools, although not necessarily related to physical activity. Implications for the TAAG intervention are presented. PMID:16397156

How Much Physical Activity Do Adults Need?

MedlinePlus

... Physical Activity Overweight & Obesity Healthy Weight Breastfeeding Micronutrient Malnutrition State and Local Programs How much physical activity ... Physical Activity Overweight & Obesity Healthy Weight Breastfeeding Micronutrient Malnutrition State and Local Programs File Formats Help: How ...

A Million Steps: Developing a Health Promotion Program at the Workplace to Enhance Physical Activity.

PubMed

González-Dominguez, María Eugenia; Romero-Sánchez, José Manuel; Ares-Camerino, Antonio; Marchena-Aparicio, Jose Carlos; Flores-Muñoz, Manuel; Infantes-Guzmán, Inés; León-Asuero, José Manuel; Casals-Martín, Fernando

2017-11-01

The workplace is a key setting for the prevention of occupational risks and for promoting healthy activities such as physical activity. Developing a physically active lifestyle results in many health benefits, improving both well-being and quality of life. This article details the experience of two Spanish companies that implemented a program to promote physical exercise in the workplace, called "A Million Steps." This program aimed to increase the physical activity of participants, challenging them to reach at least a million steps in a month through group walks. Participant workers reached the set goal and highlighted the motivational and interpersonal functions of the program.

Incentive program to strengthen motivation for increasing physical activity via conjoint analysis.

PubMed

Matsushita, Munehiro; Harada, Kazuhiro; Arao, Takashi

2017-01-01

Objectives　Promoting physical activity is a key public health issue. Incentive programs have attracted attention as a technique for promoting physical activity. For the use of effective incentives, there is a need to clarify the most effective incentive program conditions for the promotion of physical activity. Therefore, the present study used the conjoint analysis to examine the effective incentive program conditions for strengthening the motivation to increase physical activity.Methods　Data on 1,998 subjects (aged 40-74) were analyzed. The main variables in this study were physical activity (IPAQ-Short Form) and the strengthening of motivation to increase physical activity. The incentive programs that were implemented, comprised four factors: 1) cash equivalents (1,000 yen, 2,000 yen, and 3,000 yen); 2) duration between increase in physical activity and receipt of the incentive (1, 2, or 3 months); 3) method to record the physical activity (recording sheet, recording website, and automatic pedometer recording); and 4) lottery (yes or no). Eleven incentive programs were created, which was the minimum number required for comparison of these factors and levels. The average importance of each of the four factors was calculated to compare their contributions to the strengthening of the motivation to increase physical activity. The utility of each level was also calculated to compare their contributions to the strengthening of motivation. All statistics were stratified by age (≤65 years and 65+ years) and physical activity (<150 min/week, 150+ min/week) for additional analysis.Results　Cash incentives and the lottery ranked equally on average importance, followed by duration and recording methods. Utility was higher for each factor, as follows: 1) more valuable cash incentives, 2) shorter duration, 3) automatic pedometer recording, and 4) no lottery. There was no notable difference in the average importance and utility of age and physical activity.Conclusions　The results of this study suggest that no lottery and more valuable incentives were important for improving the effectiveness of incentive programs in increasing physical activity. Moreover, these two factors would be important regardless of age and physical activity levels. Further intervention studies on incentive programs for increasing physical activity considering the present results are needed.

Sandia National Laboratories California Waste Management Program Annual Report February 2008.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brynildson, Mark E.

The annual program report provides detailed information about all aspects of the Sandia National Laboratories, California (SNL/CA) Waste Management Program. It functions as supporting documentation to the SNL/CA Environmental Management System Program Manual. This annual program report describes the activities undertaken during the past year, and activities planned in future years to implement the Waste Management (WM) Program, one of six programs that supports environmental management at SNL/CA.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larsen, Barbara L.

The annual program report provides detailed information about all aspects of the SNL/CA Environmental Planning and Ecology Program for a given calendar year. It functions as supporting documentation to the SNL/CA Environmental Management System Program Manual. The 2005 program report describes the activities undertaken during the past year, and activities planned in future years to implement the Planning and Ecology Program, one of six programs that supports environmental management at SNL/CA.

A Bridge to Active Learning: A Summer Bridge Program Helps Students Maximize Their Active-Learning Experiences and the Active-Learning Experiences of Others

ERIC Educational Resources Information Center

Cooper, Katelyn M.; Ashley, Michael; Brownell, Sara E.

2017-01-01

National calls to improve student academic success in college have sparked the development of bridge programs designed to help students transition from high school to college. We designed a 2-week Summer Bridge program that taught introductory biology content in an active-learning way. Through a set of exploratory interviews, we unexpectedly…

Evaluating Active U: an internet-mediated physical activity program

PubMed Central

Buis, Lorraine R; Poulton, Timothy A; Holleman, Robert G; Sen, Ananda; Resnick, Paul J; Goodrich, David E; Palma-Davis, LaVaughn; Richardson, Caroline R

2009-01-01

Background Engaging in regular physical activity can be challenging, particularly during the winter months. To promote physical activity at the University of Michigan during the winter months, an eight-week Internet-mediated program (Active U) was developed providing participants with an online physical activity log, goal setting, motivational emails, and optional team participation and competition. Methods This study is a program evaluation of Active U. Approximately 47,000 faculty, staff, and graduate students were invited to participate in the online Active U intervention in the winter of 2007. Participants were assigned a physical activity goal and were asked to record each physical activity episode into the activity log for eight weeks. Statistics for program reach, effectiveness, adoption, and implementation were calculated using the Re-Aim framework. Multilevel regression analyses were used to assess the decline in rates of data entry and goal attainment during the program, to assess the likelihood of joining a team by demographic characteristics, to test the association between various predictors and the number of weeks an individual met his or her goal, and to analyze server load. Results Overall, 7,483 individuals registered with the Active U website (≈16% of eligible), and 79% participated in the program by logging valid data at least once. Staff members, older participants, and those with a BMI < 25 were more likely to meet their weekly physical activity goals, and average rate of meeting goals was higher among participants who joined a competitive team compared to those who participated individually (IRR = 1.28, P < .001). Conclusion Internet-mediated physical activity interventions that focus on physical activity logging and goal setting while incorporating team competition may help a significant percentage of the target population maintain their physical activity during the winter months. PMID:19744311

36 CFR 1154.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... TRANSPORTATION BARRIERS COMPLIANCE BOARD ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE ARCHITECTURAL AND TRANSPORTATION BARRIERS COMPLIANCE BOARD § 1154.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the...

Seven Activities for Enhancing the Replicability of Evidence-Based Practices. Research-to-Results Brief. Publication #2007-30

ERIC Educational Resources Information Center

Metz, Allison J. R.; Bowie, Lillian; Blase, Karen

2007-01-01

This brief will define program replication, describe the critical role of "core components" in program replication, and outline seven activities that program developers and researchers can conduct to enhance the replicability of effective program models and facilitate their adoption by other organizations and programs. Outlined is seven specific…

Physical Activity for a Healthy Weight

MedlinePlus

... Physical Activity Overweight & Obesity Healthy Weight Breastfeeding Micronutrient Malnutrition State and Local Programs Physical Activity for a ... Physical Activity Overweight & Obesity Healthy Weight Breastfeeding Micronutrient Malnutrition State and Local Programs Language: English Español (Spanish) ...

The Healthy Afterschool Activity and Nutrition Documentation Instrument

PubMed Central

Ajja, Rahma; Beets, Michael W.; Huberty, Jennifer; Kaczynski, Andrew T.; Ward, Dianne S.

2012-01-01

Background Policies call on afterschool programs to improve the physical activity and nutrition habits of youth attending. No tool exists to assess the extent to which the afterschool program environment meets physical activity and nutrition policies. Purpose To describe the development of the Healthy Afterschool Activity and Nutrition Documentation (HAAND) instrument, which consists of two subscales: Healthy Afterschool Program Index for Physical Activity (HAPI-PA) and the HAPI-Nutrition (HAPI-N). Methods Thirty-nine afterschool programs took part in the HAAND evaluation during fall/spring 2010–2011. Inter-rater reliability data were collected at 20 afterschool programs during a single site visit via direct observation, personal interview and written document review. Validity of the HAPI-PA was established by comparing HAPI-PA scores to pedometer steps collected in a subsample of 934 children attending 25 of the afterschool programs. Validity of the HAPI-N scores was compared against the mean number of times/week that fruits/vegetables (FV) and whole grains were served in the program. Results Data were analyzed in June/July 2011. Inter-rater percent agreement was 85%–100% across all items. Increased pedometer steps were associated with the presence of a written policy related to physical activity, amount/quality of staff training, use of a physical activity curriculum, and offering activities that appeal to both genders. Higher servings of FV and whole grains per week were associated with the presence of a written policy regarding the nutritional quality of snacks. Conclusions The HAAND instrument is a reliable and valid measurement tool that can be used to assess the physical activity and nutritional environment of afterschool programs. PMID:22898119

The healthy afterschool activity and nutrition documentation instrument.

PubMed

Ajja, Rahma; Beets, Michael W; Huberty, Jennifer; Kaczynski, Andrew T; Ward, Dianne S

2012-09-01

Policies call on afterschool programs to improve the physical activity and nutrition habits of youth attending. No tool exists to assess the extent to which the afterschool program environment meets physical activity and nutrition policies. To describe the development of the Healthy Afterschool Activity and Nutrition Documentation (HAAND) instrument, which consists of two subscales: Healthy Afterschool Program Index for Physical Activity (HAPI-PA) and the HAPI-Nutrition (HAPI-N). Thirty-nine afterschool programs took part in the HAAND evaluation during fall/spring 2010-2011. Inter-rater reliability data were collected at 20 afterschool programs during a single site visit via direct observation, personal interview, and written document review. Validity of the HAPI-PA was established by comparing HAPI-PA scores to pedometer steps collected in a subsample of 934 children attending 25 of the afterschool programs. Validity of the HAPI-N scores was compared against the mean number of times/week that fruits and vegetables (FV) and whole grains were served in the program. Data were analyzed in June/July 2011. Inter-rater percent agreement was 85%-100% across all items. Increased pedometer steps were associated with the presence of a written policy related to physical activity, amount/quality of staff training, use of a physical activity curriculum, and offering activities that appeal to both genders. Higher servings of FV and whole grains per week were associated with the presence of a written policy regarding the nutritional quality of snacks. The HAAND instrument is a reliable and valid measurement tool that can be used to assess the physical activity and nutritional environment of afterschool programs. Copyright © 2012 American Journal of Preventive Medicine. Published by Elsevier Inc. All rights reserved.

Multi-site randomized controlled trial of a child-centered physical activity program, a parent-centered dietary-modification program, or both in overweight children: the HIKCUPS study.

PubMed

Okely, Anthony D; Collins, Clare E; Morgan, Philip J; Jones, Rachel A; Warren, Janet M; Cliff, Dylan P; Burrows, Tracy L; Colyvas, Kim; Steele, Julie R; Baur, Louise A

2010-09-01

To evaluate whether a child-centered physical activity program, combined with a parent-centered dietary program, was more efficacious than each treatment alone, in preventing unhealthy weight-gain in overweight children. An assessor-blinded randomized controlled trial involving 165 overweight/obese 5.5- to 9.9- year-old children. Participants were randomly assigned to 1 of 3 interventions: a parent-centered dietary program (Diet); a child-centered physical activity program (Activity); or a combination of both (Diet+Activity). All groups received 10 weekly face-to-face sessions followed by 3 monthly relapse-prevention phone calls. Analysis was by intention-to-treat. The primary outcome was change in body mass index z-score at 6 and 12 months (n=114 and 106, respectively). Body mass index z-scores were reduced at 12-months in all groups, with the Diet (mean [95% confidence interval]) (-0.39 [-0.51 to 0.27]) and Diet + Activity (-0.32, [-0.36, -0.23]) groups showing a greater reduction than the Activity group (-0.17 [-0.28, -0.06]) (P=.02). Changes in other outcomes (waist circumference and metabolic profile) were not statistically significant among groups. Relative body weight decreased at 6 months and was sustained at 12 months through treatment with a child-centered physical activity program, a parent-centered dietary program, or both. The greatest effect was achieved when a parent-centered dietary component was included. Copyright (c) 2010 Mosby, Inc. All rights reserved.

7 CFR 3430.1007 - Planning activities.

Code of Federal Regulations, 2012 CFR

2012-01-01

... ADMINISTRATIVE PROVISIONS Sun Grant Program § 3430.1007 Planning activities. (a) Required plan. The Centers and... program. Proposals submitted to the Sun Grant Program must be sufficiently detailed and of high enough...

7 CFR 3430.1007 - Planning activities.

Code of Federal Regulations, 2013 CFR

2013-01-01

... ADMINISTRATIVE PROVISIONS Sun Grant Program § 3430.1007 Planning activities. (a) Required plan. The Centers and... program. Proposals submitted to the Sun Grant Program must be sufficiently detailed and of high enough...

7 CFR 3430.1007 - Planning activities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... ADMINISTRATIVE PROVISIONS Sun Grant Program § 3430.1007 Planning activities. (a) Required plan. The Centers and... program. Proposals submitted to the Sun Grant Program must be sufficiently detailed and of high enough...

7 CFR 3430.1007 - Planning activities.

Code of Federal Regulations, 2014 CFR

2014-01-01

... ADMINISTRATIVE PROVISIONS Sun Grant Program § 3430.1007 Planning activities. (a) Required plan. The Centers and... program. Proposals submitted to the Sun Grant Program must be sufficiently detailed and of high enough...

The 1992 NASA/ASEE Summer Faculty Fellowship Program

NASA Technical Reports Server (NTRS)

1992-01-01

This is the administrative report for the 1992 NASA/ASEE Summer Faculty Fellowship Program which was held at the George C. Marshall Space Flight Center (MSFC) for the 28th consecutive year. The nominal starting and finishing dates for the ten week program were June 1, 1992 through August 7, 1992. The program was sponsored by NASA Headquarters, Washington, D.C., and operated under the auspices of the American Society for Engineering Education (ASEE). The program was one of eight such programs at eight NASA centers sponsored and funded by NASA Headquarters. The basic objectives of the program are the following: (1) to further the professional knowledge of qualified engineering and science faculty members; (2) to stimulate an exchange of ideas between participants and NASA; (3) to enrich and refresh the research and teaching activities at the participants' institutions; and (4) to contribute to the research objectives of the NASA centers. The major activities of the 1992 program were the following: (1) recruitment, selection, and assignment of faculty fellows; (2) research performed by the participants in collaboration with the MSFC colleague; (3) a seminar and tour program aimed at providing information concerning activities at MSFC; (4) an activities program of a social/non-technical nature aimed at providing the fellows and their families a means of learning about the MSFC/Huntsville area; and (5) preparation of a volume containing the written reports of the details of the research performed by each of the summer faculty. The success of the 1992 program activities in meeting the stated objectives was measured through questionnaires, which were filled out by participants and their MSFC colleagues. The following sections describe the major activities in more detail and the results of the questionnaires are summarized showing that the 1992 program was highly successful. This year's program also included 19 participants in the Summer Teacher Enrichment Program (STEP) which is comprised of middle school and high school math and science teachers.

'Physical activity at home (PAAH)', evaluation of a group versus home based physical activity program in community dwelling middle aged adults: rationale and study design.

PubMed

Freene, Nicole; Waddington, Gordon; Chesworth, Wendy; Davey, Rachel; Goss, John

2011-11-24

It is well recognised that the adoption and longer term adherence to physical activity by adults to reduce the risk of chronic disease is a challenge. Interventions, such as group and home based physical activity programs, have been widely reported upon. However few studies have directly compared these interventions over the longer term to determine their adherence and effectiveness. Participant preference for home based or group interventions is important. Some evidence suggests that home based physical activity programs are preferred by middle aged adults and provide better long term physical activity adherence. Physiotherapists may also be useful in increasing physical activity adherence, with limited research on their impact. 'Physical Activity at Home' is a 2 year pragmatic randomised control trial, with a non-randomised comparison to group exercise. Middle-aged adults not interested in, or unable to attend, a group exercise program will be targeted. Sedentary community dwelling 50-65 year olds with no serious medical conditions or functional impairments will be recruited via two mail outs using the Australian federal electoral roll. The first mail out will invite participants to a 6 month community group exercise program. The second mail out will be sent to those not interested in the group exercise program inviting them to take part in a home based intervention. Eligible home based participants will be randomised into a 6 month physiotherapy-led home based physical activity program or usual care. Outcome measures will be taken at baseline, 6, 12, 18 and 24 months. The primary outcome is physical activity adherence via exercise diaries. Secondary outcomes include the Active Australia Survey, accelerometry, aerobic capacity (step test), quality of life (SF-12v2), blood pressure, waist circumference, waist-to-hip ratio and body mass index. Costs will be recorded prospectively and qualitative data will be collected. The planned 18 month follow-up post intervention will provide an indication of the effectiveness of the group and home based interventions in terms of adherence to physical activity, health benefits and cost. If the physiotherapy-led home based physical activity program is successful it could provide an alternative option for physical activity program delivery across a number of settings. Australia and New Zealand Clinical Trials Register (ANZCTR): ACTRN12611000890932.

Logistics planning for phased programs.

NASA Technical Reports Server (NTRS)

Cook, W. H.

1973-01-01

It is pointed out that the proper and early integration of logistics planning into the phased program planning process will drastically reduce these logistics costs. Phased project planning is a phased approach to the planning, approval, and conduct of major research and development activity. A progressive build-up of knowledge of all aspects of the program is provided. Elements of logistics are discussed together with aspects of integrated logistics support, logistics program planning, and logistics activities for phased programs. Continuing logistics support can only be assured if there is a comprehensive sequential listing of all logistics activities tied to the program schedule and a real-time inventory of assets.

Shared use of school facilities with community organizations and afterschool physical activity program participation: a cost-benefit assessment.

PubMed

Kanters, Michael A; Bocarro, Jason N; Filardo, Mary; Edwards, Michael B; McKenzie, Thomas L; Floyd, Myron F

2014-05-01

Partnerships between school districts and community-based organizations to share school facilities during afterschool hours can be an effective strategy for increasing physical activity. However, the perceived cost of shared use has been noted as an important reason for restricting community access to schools. This study examined shared use of middle school facilities, the amount and type of afterschool physical activity programs provided at middle schools together with the costs of operating the facilities. Afterschool programs were assessed for frequency, duration, and type of structured physical activity programs provided and the number of boys and girls in each program. School operating costs were used to calculate a cost per student and cost per building square foot measure. Data were collected at all 30 middle schools in a large school district over 12 months in 2010-2011. Policies that permitted more use of school facilities for community-sponsored programs increased participation in afterschool programs without a significant increase in operating expenses. These results suggest partnerships between schools and other community agencies to share facilities and create new opportunities for afterschool physical activity programs are a promising health promotion strategy. © 2014, American School Health Association.

A theory-informed, process-oriented Resident Scholarship Program

PubMed Central

Thammasitboon, Satid; Darby, John B.; Hair, Amy B.; Rose, Karen M.; Ward, Mark A.; Turner, Teri L.; Balmer, Dorene F.

2016-01-01

Background The Accreditation Council for Graduate Medical Education requires residency programs to provide curricula for residents to engage in scholarly activities but does not specify particular guidelines for instruction. We propose a Resident Scholarship Program that is framed by the self-determination theory (SDT) and emphasize the process of scholarly activity versus a scholarly product. Methods The authors report on their longitudinal Resident Scholarship Program, which aimed to support psychological needs central to SDT: autonomy, competence, and relatedness. By addressing those needs in program aims and program components, the program may foster residents’ intrinsic motivation to learn and to engage in scholarly activity. To this end, residents’ engagement in scholarly processes, and changes in perceived autonomy, competence, and relatedness were assessed. Results Residents engaged in a range of scholarly projects and expressed positive regard for the program. Compared to before residency, residents felt more confident in the process of scholarly activity, as determined by changes in increased perceived autonomy, competence, and relatedness. Scholarly products were accomplished in return for a focus on scholarly process. Conclusions Based on our experience, and in line with the SDT, supporting residents’ autonomy, competence, and relatedness through a process-oriented scholarship program may foster the curiosity, inquisitiveness, and internal motivation to learn that drives scholarly activity and ultimately the production of scholarly products. PMID:27306995

A theory-informed, process-oriented Resident Scholarship Program.

PubMed

Thammasitboon, Satid; Darby, John B; Hair, Amy B; Rose, Karen M; Ward, Mark A; Turner, Teri L; Balmer, Dorene F

2016-01-01

The Accreditation Council for Graduate Medical Education requires residency programs to provide curricula for residents to engage in scholarly activities but does not specify particular guidelines for instruction. We propose a Resident Scholarship Program that is framed by the self-determination theory (SDT) and emphasize the process of scholarly activity versus a scholarly product. The authors report on their longitudinal Resident Scholarship Program, which aimed to support psychological needs central to SDT: autonomy, competence, and relatedness. By addressing those needs in program aims and program components, the program may foster residents' intrinsic motivation to learn and to engage in scholarly activity. To this end, residents' engagement in scholarly processes, and changes in perceived autonomy, competence, and relatedness were assessed. Residents engaged in a range of scholarly projects and expressed positive regard for the program. Compared to before residency, residents felt more confident in the process of scholarly activity, as determined by changes in increased perceived autonomy, competence, and relatedness. Scholarly products were accomplished in return for a focus on scholarly process. Based on our experience, and in line with the SDT, supporting residents' autonomy, competence, and relatedness through a process-oriented scholarship program may foster the curiosity, inquisitiveness, and internal motivation to learn that drives scholarly activity and ultimately the production of scholarly products.

Improving practice with integration of patient directed activity during inpatient rehabilitation.

PubMed

Trammell, Molly; Kapoor, Priyanka; Swank, Chad; Driver, Simon

2017-01-01

Early initiation of rehabilitation following stroke promotes better long-term outcomes than delayed onset, emphasizing the importance of inpatient therapy. However, literature indicates that following stroke individuals in inpatient rehabilitation spend the majority of their day in their bedroom and inactive. Consequently, since amount of functional activity is posited to relate to outcomes, the current rehabilitation model needs to be challenged with innovative solutions to maximize recovery. In an attempt to promote greater activity and higher doses of therapy during inpatient rehabilitation, we implemented the "Patient Directed Activity Program" to facilitate specific movement and improve outcomes for patients post stroke. Our interdisciplinary activity program was conceptualized on a theoretical model for stroke recovery and principles of experience-dependent neural plasticity. The "Patient Directed Activity Program" includes distinct activity stations designed to increase repetition, stimulation, attention, and activity of the affected upper extremities, lower extremities, and trunk. Each task-specific activity was easily graded to achieve moderate- to high-intensity. The activity program prescribed individuals up to three additional 30-minute bouts of activities daily that were to be completed independently, and in addition to standard of care. Clinical application: After implementing this program in our facility for one year as a quality improvement project, the intervention has been delivered as an Institutional Review Board approved randomized controlled trial (Clinical Trial #NCT02446197). Challenges with people and facilities have been overcome, resulting in a feasible program that can be delivered in an inpatient setting. High satisfaction has been reported by patients and clinicians.

Do Training Programs Work? An Assessment of Pharmacists Activities in the Field of Chemical Dependency.

ERIC Educational Resources Information Center

Brooks, Valerie G.; Brock, Tina Penick; Ahn, Jungeun

2001-01-01

Seeks to determine if pharmacists who attended a chemical dependency training program were performing more chemical dependency related activities. Results reveal that participants were more likely to perform the following activities: lecture to community groups about chemical dependency; participate in a pharmacists' recovery program; provide…

39 CFR 255.3 - Nondiscrimination under any program or activity conducted by the Postal Service.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 39 Postal Service 1 2010-07-01 2010-07-01 false Nondiscrimination under any program or activity conducted by the Postal Service. 255.3 Section 255.3 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION ACCESS OF PERSONS WITH DISABILITIES TO POSTAL SERVICE PROGRAMS, ACTIVITIES...

Evaluating Active Parental Consent Procedures for School Programming: Addressing the Sensitive Topic of Suicide Prevention

ERIC Educational Resources Information Center

Totura, Christine M. Wienke; Kutash, Krista; Labouliere, Christa D.; Karver, Marc S.

2017-01-01

Background: Suicide is the second leading cause of death for adolescents. Whereas school-based prevention programs are effective, obtaining active consent for youth participation in public health programming concerning sensitive topics is challenging. We explored several active consent procedures for improving participation rates. Methods: Five…

77 FR 46606 - Enforcement of Nondiscrimination on the Basis of Disability in Programs and Activities Conducted...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-06

..., and specific learning disabilities. (3) Diseases and conditions such as orthopedic, visual, speech and... of Nondiscrimination on the Basis of Disability in Programs and Activities Conducted by the Bureau of... disability in programs or activities conducted by the Bureau of Consumer Financial Protection. It sets forth...

Employee engagement factors that affect enrollment compared with retention in two coaching programs--the ACTIVATE study.

PubMed

Terry, Paul E; Fowles, Jinnet B; Harvey, Lisa

2010-06-01

This article describes enrollment and retention results from a randomized controlled trial that tested differences between a traditional worksite health promotion program and an activated consumer program on health behaviors and health status. A control arm was included. Baseline survey and clinical data were collected from 631 of 1628 eligible employees (39% response rate) between March and June of 2005. Retention data were collected in March 2007-12 months into an 18-month program. At baseline, participants in the 6 groups (3 arms in each of 2 companies) were comparable in health status but not in patient activation status. Enrollment of high-risk employees into the 2 individualized coaching programs (one focused on traditional health promotion, the other focused on activated consumer navigation) varied significantly by industry type, smoking status, and patient activation. In contrast, retention in the coaching programs was related to sex, age, and industry type. Our findings suggest that one set of strategies may be needed to encourage program enrollment while a distinctly different set of strategies may be needed to sustain participation.

44 CFR 19.400 - Education programs or activities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 44 Emergency Management and Assistance 1 2011-10-01 2011-10-01 false Education programs or..., DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

45 CFR 2522.900 - What definitions apply to this subpart?

Code of Federal Regulations, 2010 CFR

2010-10-01

... safe place for children is not tutoring, even if some of the program activities focus on homework help. ... NATIONAL AND COMMUNITY SERVICE AMERICORPS PARTICIPANTS, PROGRAMS, AND APPLICANTS Program Management... someone engaged in other academic support activities, such as mentoring and after-school program support...

44 CFR 19.400 - Education programs or activities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Education programs or..., DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

40 CFR 35.6225 - Activities eligible for funding under Core Program Cooperative Agreements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Core Program Cooperative Agreements. 35.6225 Section 35.6225 Protection of Environment ENVIRONMENTAL... Superfund State Contracts for Superfund Response Actions Core Program Cooperative Agreements § 35.6225 Activities eligible for funding under Core Program Cooperative Agreements. (a) To be eligible for funding...

41 CFR 102-74.100 - What are conservation programs?

Code of Federal Regulations, 2014 CFR

2014-01-01

... programs are programs that improve energy and water efficiency and promote the use of solar and other renewable energy. These programs must promote and maintain an effective source reduction activity (reducing consumption of resources such as energy, water, and paper), resource recovery activity (obtaining materials...

41 CFR 102-74.100 - What are conservation programs?

Code of Federal Regulations, 2012 CFR

2012-01-01

... programs are programs that improve energy and water efficiency and promote the use of solar and other renewable energy. These programs must promote and maintain an effective source reduction activity (reducing consumption of resources such as energy, water, and paper), resource recovery activity (obtaining materials...

41 CFR 102-74.100 - What are conservation programs?

Code of Federal Regulations, 2013 CFR

2013-07-01

... programs are programs that improve energy and water efficiency and promote the use of solar and other renewable energy. These programs must promote and maintain an effective source reduction activity (reducing consumption of resources such as energy, water, and paper), resource recovery activity (obtaining materials...

40 CFR 35.6225 - Activities eligible for funding under Core Program Cooperative Agreements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Core Program Cooperative Agreements. 35.6225 Section 35.6225 Protection of Environment ENVIRONMENTAL... Superfund State Contracts for Superfund Response Actions Core Program Cooperative Agreements § 35.6225 Activities eligible for funding under Core Program Cooperative Agreements. (a) To be eligible for funding...

5 CFR 1207.150 - Program accessibility: Existing facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... facilities. 1207.150 Section 1207.150 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION... CONDUCTED BY THE MERIT SYSTEMS PROTECTION BOARD § 1207.150 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larsen, Barbara L.

The annual program report provides detailed information about all aspects of the Sandia National Laboratories, California (SNL/CA) Environmental Planning and Ecology Program for a given calendar year. It functions as supporting documentation to the SNL/CA Environmental Management System Program Manual. The 2006 program report describes the activities undertaken during the past year, and activities planned in future years to implement the Planning and Ecology Program, one of six programs that supports environmental management at SNL/CA.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larsen, Barbara L.

The annual program report provides detailed information about all aspects of the Sandia National Laboratories, California (SNL/CA) Environmental Planning and Ecology Program for a given calendar year. It functions as supporting documentation to the SNL/CA Environmental Management System Program Manual. The program report describes the activities undertaken during the past year, and activities planned in future years to implement the Planning and Ecology Program, one of six programs that supports environmental management at SNL/CA.

U.S. commercial space activities - Returning the U.S. to preeminence in space

NASA Technical Reports Server (NTRS)

Stone, Barbara A.

1987-01-01

The current status of NASA's activities related to the commercial development of space is reviewed with particular reference to the emerging new commercial space activities and the post-Challenger policy developments affecting space commerce. The discussion covers the development of U.S. private sector launching capabilities, cooperative agreements with the private sector, the NASA technology utilization program, the technology applications activities of the Office of Commercial Programs, and the activities of the Centers for the Commercial Development of Space program.

A Randomized Controlled Trial of an Activity Specific Exercise Program for Individuals With Alzheimer Disease in Long-term Care Settings

PubMed Central

Roach, Kathryn E.; Tappen, Ruth M.; Kirk-Sanchez, Neva; Williams, Christine L.; Loewenstein, David

2011-01-01

Objective To determine whether an activity specific exercise program could improve ability to perform basic mobility activities in long-term care residents with Alzheimer disease (AD). Design Randomized, controlled, single-blinded clinical trial. Setting Residents of 7 long-term care facilities. Participants Eighty-two long-term care residents with mild to severe AD. Intervention An activity specific exercise program was compared to a walking program and to an attention control. Measurements Ability to perform bed mobility and transfers were assessed using the subscales of the Acute Care Index of Function; functional mobility was measured using the 6-Minute Walk test. Results Subjects receiving the activity specific exercise program improved in ability to perform transfers, whereas subjects in the other 2 groups declined. PMID:21937893

Service and Methods Demonstrations Program Summary Report

DOT National Transportation Integrated Search

1981-12-01

This report summarizes the activities and accomplishments of the UMTA Service and Methods Demonstrations Program for FY 1979, 1980, and 1981. Overall program objectives, current activities, and future directions are briefly described. Current demonst...

Open-Access Physical Activity Programs for Older Adults: A Pragmatic and Systematic Review.

PubMed

Balis, Laura E; Strayer, Thomas; Ramalingam, NithyaPriya; Wilson, Meghan; Harden, Samantha M

2018-01-10

Open-access, community-based programs are recommended to assist older adults in meeting physical activity guidelines, but the characteristics, impact, and scalability of these programs is less understood. The Land-Grant University Cooperative Extension System, an organization providing education through county-based educators, functions as a delivery system for these programs. A systematic review was conducted to determine characteristics of effective older adult physical activity programs and the extent to which programs delivered in Extension employ these characteristics. A systematic review of peer-reviewed and grey literature was conducted from August 2016 to February 2017. The review was limited to open-access (available to all), community-based physical activity interventions for older adults (≥65 years of age). The peer-reviewed literature search was conducted in PubMed and EBSCOhost; the grey literature search for Extension interventions was conducted through Extension websites, Land-Grant Impacts, and the Journal of Extension. Sixteen peer-reviewed studies and 17 grey literature sources met inclusion criteria and were analyzed. Peer-reviewed and Extension programs were similar in their limited use of behavioral theories and group-based strategies. Compared to Extension programs, those in the peer-reviewed literature were more likely to use a combination of physical activity components and be delivered by trained professionals. The results indicate notable differences between peer-reviewed literature and Extension programs and present an opportunity for Extension programs to more effectively use evidence-based program characteristics, including behavioral theories and group dynamics, a combination of physical activity components, and educator/agent-trained delivery agents. © The Author(s) 2017. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Participant Perceptions of Character Concepts in a Physical Activity-Based Positive Youth Development Program.

PubMed

Riciputi, Shaina; McDonough, Meghan H; Ullrich-French, Sarah

2016-10-01

Physical activity-based positive youth development (PYD) programs often aim to foster character development. This study examined youth perspectives of character development curricula and the impact these activities have on their lives within and beyond the program. This case study examined youth from low-income families in a physical activity-based summer PYD program that integrated one character concept (respect, caring, responsibility, trust) in each of 4 weeks. Participants (N = 24) included a cross section of age, gender, ethnicity, and past program experience. Semi-structured interviews were analyzed using inductive thematic analysis and constant comparative methods. Thirteen themes were grouped in four categories: building highquality reciprocal relationships; intrapersonal improvement; moral reasoning and understanding; and rejection, resistance, and compliance. The findings provide participant-centered guidance for understanding youth personal and social development through physical activity in ways that are meaningful to participants, which is particularly needed for youth in low-income communities with limited youth programming.

Comparing responses to horticultural-based and traditional activities in dementia care programs.

PubMed

Jarrott, Shannon E; Gigliotti, Christina M

2010-12-01

Engaging persons with dementia in meaningful activities supports well-being; however, care staff are challenged to implement age- and ability-appropriate activities in a group setting. We compared a randomly assigned treatment group, who received horticultural therapy-based (HT-based) programming to a comparison group, who engaged in traditional activities (TA) programming, on engagement and affect. Horticultural therapy-based programming was implemented twice weekly at 4 treatment sites for 6 weeks, while regular TA were observed at comparison sites. Results revealed no differences between groups on affective domains. Levels of adaptive behavior differed between the groups, with the treatment group demonstrating higher levels of active, passive, and other engagement and the comparison group demonstrating higher levels of self-engagement. Our results highlight the value of HT-based programs and the importance of simultaneously capturing participants' affective and behavioral responses. Theoretical and practical considerations about the facilitation of and context in which the programming occurs are discussed.

NASULGC Council on Extension, Committee on Program Innovation and Action Oriented Research; 1968 Report. Part 1. Program Innovations. Part 2. Action Oriented Research.

ERIC Educational Resources Information Center

National Association of State Universities and Land Grant Colleges, Washington, DC.

This report on extension activities of member institutions of the National Association of State Universities and Land Grant Colleges describes 91 program innovations and action oriented research activities. Objectives, sponsorship, program evaluations, and other data are cited for program innovations in such areas as continuing medical education,…

Final report : for the period of December 1999 through November 30, 2000 : Florida Transit Training Program (1999/2000) : Florida Technical Assistance Program (1999/2000)

DOT National Transportation Integrated Search

2000-01-01

The following progress report is intended to highlight the significant activities of the Florida Transit Training Program and Florida Technical Assistant Program. The following progress report is intended to highlight the significant activities of th...

Program Activity/Training Plans. STIP II (Skill Training Improvement Programs Round II).

ERIC Educational Resources Information Center

Los Angeles Community Coll. District, CA.

Detailed operational guidelines, training objectives, and learning activities are provided for the Los Angeles Community College District's Skill Training Improvement Programs (STIP II), which are designed to train students for immediate employment. The first of four reports covers Los Angeles Southwest College's computer programming trainee…

34 CFR 642.10 - Activities the Secretary assists under the Training Program.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Secretary assists under the Training Program. (a) A Training Program project trains the staff and leadership... 34 Education 3 2010-07-01 2010-07-01 false Activities the Secretary assists under the Training... (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO...

Business process modeling for the Virginia Department of Transportation : a demonstration with the integrated six-year improvement program and the statewide transportation improvement program.

DOT National Transportation Integrated Search

2005-01-01

This effort demonstrates business process modeling to describe the integration of particular planning and programming activities of a state highway agency. The motivations to document planning and programming activities are that: (i) resources for co...

75 FR 64322 - Notice of Formula Allocations and Program Requirements for Neighborhood Stabilization Program...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-19

... notice also notes statutory issues affecting program design and implementation. Note: This notice is... gave HUD broad waiver and alternative requirement authority, which HUD used in designing NSP program... activity produces housing, how the design of the activity will ensure continued affordability; v. How the...

76 FR 43253 - Agency Information Collection Activities: Proposed Collection: Comment Request: FNS-583...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-20

... Nutrition Assistance Program Employment and Training Program Activity Report AGENCY: Food and Nutrition... the Supplemental Nutrition Assistance Program (SNAP) Employment and Training (E&T) Program, currently... hours. An increase in the estimated number of annual work registrants accounts for most of the increase...

75 FR 76677 - Financial Crimes Enforcement Network: Anti-Money Laundering Program and Suspicious Activity...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-09

...: Anti-Money Laundering Program and Suspicious Activity Report Filing Requirements for Residential... loan or finance companies for the purpose of requiring them to establish anti-money laundering programs...-money laundering program requirements on financial institutions.\\3\\ The authority of the Secretary to...

77 FR 8148 - Anti-Money Laundering Program and Suspicious Activity Report Filing Requirements for Residential...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-14

... 1506-AB02 Anti-Money Laundering Program and Suspicious Activity Report Filing Requirements for... finance companies for the purpose of requiring them to establish anti-money laundering programs and report... Secretary is authorized to impose anti-money laundering (``AML'') program requirements on financial...

Formative research and strategic development of a physical activity component to a social marketing campaign for obesity prevention in preschoolers.

PubMed

Bellows, Laura; Anderson, Jennifer; Gould, Susan Martin; Auld, Garry

2008-06-01

The prevalence of overweight in childhood, including preschoolers, continues to rise. While efforts focusing on school-aged children are encouraging, obesity prevention programs to address nutrition and physical activity in the child care center are lacking. Food Friends is a successfully evaluated nutrition program aimed at enhancing preschoolers' food choices, the addition of a physical activity program would improve the programs overall efforts to establish healthful habits early in life. This study describes the formative research conducted with secondary influencers of preschoolers-teachers and parents-for the development of a physical activity program. Key informant interviews and focus group discussions were conducted with preschool teachers and parents, respectively, to examine current physical activity practices, as well as attitudes, opinions, and desired wants and needs for physical activity materials. Findings illustrate that teachers provided physical activity; however, most did not use a structured program. Teachers identified time, space and equipment as barriers to providing activity in their classroom. Focus group findings identified activities of preschoolers', parents' perceptions of the adequacy of activity levels, and items to help parents engage their children in more physical activity. Barriers were also identified by parents and included time, safety, inclement weather, and lack of knowledge and self-efficacy. Findings from this formative research were used to develop a marketing strategy to guide the development of a physical activity component, Food Friends Get Movin' with Mighty Moves , as part of a larger social marketing campaign aimed to decrease the risk for obesity in low-income preschoolers.

Changes in physical functioning in the Active Living Every Day program of the Active for Life Initiative®.

PubMed

Baruth, Meghan; Wilcox, Sara; Wegley, Stacy; Buchner, David M; Ory, Marcia G; Phillips, Alisa; Schwamberger, Karen; Bazzarre, Terry L

2011-09-01

Physical activity can prevent or delay the onset of physical functional limitations in older adults. There are limited data that evidence-based physical activity interventions can be successfully translated into community programs and result in similar benefits for physical functioning. The purpose of this study is to measure the effects of the Active Living Every Day program on physical functioning and physical functional limitations in a diverse sample of older adults. As a part of the Active for Life initiative, the Council on Aging of Southwestern Ohio implemented Active Living Every Day (ALED), a group-based lifestyle behavior change program designed to increase physical activity. Performance-based physical functioning tests (30-s Chair Stand Test, eight Foot Up-and-Go Test, Chair Sit-and-Reach Test, 30-Foot Walk Test) were administered to participants at baseline and posttest. Baseline to post-program changes in physical functioning and impairment status were examined with repeated measures analysis of covariance. Interactions tested whether change over time differed according to race/ethnicity, body mass index (BMI), and baseline impairment status. Participants significantly increased their performance in all four physical functioning tests. The percentage of participants classified as "impaired" according to normative data significantly decreased over time. Physical functioning improved regardless of BMI, race/ethnicity, or baseline impairment status. ALED is an example of an evidenced-based physical activity program that can be successfully translated into community programs and result in significant and clinically meaningful improvements in performance-based measures of physical functioning.

Risk assessment for the reintroduction of anadromous salmonids upstream of Chief Joseph and Grand Coulee Dams, Northeastern Washington

USGS Publications Warehouse

Hardiman, Jill M.; Breyta, Rachel B.; Haskell, Craig A.; Ostberg, Carl O.; Hatten, James R.; Connolly, Patrick J.

2017-09-12

The Upper Columbia United Tribes (UCUT; Spokane, Colville, Kootenai, Coeur d’Alene, and Kalispel Tribes) and Washington Department of Fish and Wildlife want to reintroduce anadromous salmonids to their historical range to restore ecosystem function and lost cultural and spiritual relationships in the upper Columbia River, northeastern Washington. The UCUT contracted with the U.S. Geological Survey to assess risks to resident taxa (existing fish populations in the reintroduction area upstream of Chief Joseph and Grand Coulee Dams) and reintroduced salmon associated with reintroduction. We developed a risk assessment framework for reintroduction of anadromous salmonids upstream of Chief Joseph and Grand Coulee Dams. To accomplish this goal, we applied strategies identified in previous risk assessment frameworks for reintroduction. The risk assessment is an initial step towards an anadromous reintroduction strategy. An initial list of potential donor sources for reintroduction species was developed from previous published sources for Chinook Salmon (Oncorhynchus tshawytscha) donors in the Transboundary Reach of the Columbia River, British Columbia; an ecological risk assessment of upper Columbia River hatchery programs on non-target taxa of concern; and a review of existing hatchery programsDuring two workshops, we further identified and ranked potential donor sources of anadromous Redband Trout (steelhead; O. mykiss), Chinook Salmon, Sockeye Salmon (O. nerka), and Coho Salmon (O. kisutch). We also identified resident fish populations of interest and their primary habitat, location, status, and pathogen concerns to determine the potential risks of reintroduction. Species were deemed of interest based on resource management and potential interactions (that is, genetics, competition, and predation) with introduced species. We developed tables of potential donors by species and characterized potential sources (hatchery and natural origins), populations (individual runs), broodstock management and history, and potential constraints (that is, Endangered Species Act [ESA] listing, Evolutionarily Significant Unit concerns, pathogens, and availability). During the workshops, a group of regional fisheries and topic experts subjectively ranked the relative risks of pathogens, genetic effects, predation, and competition to resident fish and reintroduced salmonids. We assessed the pathogen risk of each potential donor for introducing new pathogens and the increased burden to existing pathogens for resident species upstream of the dams. We considered genetic risks to resident and downstream conspecifics and ecological impacts, including competition for food and space, predator-prey interactions, and ecosystem benefits/impacts. Each reintroduced species donor source was ranked based on abundance/viability (demographic risk to source and feasibility of collection), ancestral/genetic similarity (evolutionary similarity to historical populations), local adaptation (geographic proximity/similarity of source conditions to reintroduction conditions), and life history compatibility (including migration; spawn timing; and relative usage of reservoir, main-stem, or tributary habitats) with environmental conditions in the reintroduction area. We synthesized this information by species for all potential donors, in which an overall score and ranking system was established for decision support in donor selection for reintroduction into the upper Columbia River. We also provided information outside the ranking process by:Identifying predator-prey interactions and competition for food and space among species,Developing a decision support framework for donor selection, andProviding decision support for reintroduction strategies.

Evaluation of the Science Enrichment Activities (SEA) Program: A Decision Oriented Model.

ERIC Educational Resources Information Center

Linn, Marcia C.

1978-01-01

Three questions guided an evaluation of sixth and eighth grade science enrichment activities: (1) Does a free choice interactive program affect cognitive abilities? (2) Do students in a free choice program make predictable selections of activities based on their age, sex, or ability level? and (3) Are specific student choices associated with…

13 CFR 101.401 - What programs and activities of SBA are subject to this subpart?

Code of Federal Regulations, 2010 CFR

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What programs and activities of SBA are subject to this subpart? 101.401 Section 101.401 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Intergovernmental Partnership § 101.401 What programs and activities of SBA are...

13 CFR 101.401 - What programs and activities of SBA are subject to this subpart?

Code of Federal Regulations, 2011 CFR

2011-01-01

... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What programs and activities of SBA are subject to this subpart? 101.401 Section 101.401 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Intergovernmental Partnership § 101.401 What programs and activities of SBA are...

Physical Activity Intensity, Lesson Context, and Teacher Interactions during an Unstructured Afterschool Physical Activity Program

ERIC Educational Resources Information Center

Behrens, Timothy K.; Miller, Daniel J.; Schuna, John M.; Liebert, Mina L.

2015-01-01

Background: Afterschool programs are promising arenas to improve youth physical activity (PA) levels. During the school year for 2012-2013, 5 elementary schools from a low-socioeconomic status (SES) school district in southern Colorado participated in evaluation of the afterschool program entitled Keep It Moving! (KIM). Methods: In this…

Teaching an Old Dog New Tricks: Past, Present, and Future Priorities for Higher Education Physical Activity Programs

ERIC Educational Resources Information Center

Stapleton, Drue T.; Taliaferro, Andrea R.; Bulger, Sean M.

2017-01-01

Physical education programs in colleges/universities have been called on to provide students with opportunities to develop an appreciation for, and increase participation in, lifetime physical activity. Higher Education Physical Activity Programs (HEPAPs) have evolved over the past 100 years in response to changing societal and institutional…

NSF Factbook. Guide to National Science Foundation Programs and Activities.

ERIC Educational Resources Information Center

Renetzky, Alvin, Ed.; Flynn, Barbara J., Ed.

This publication is a thorough guide to National Science Foundation (NSF) programs and activities. Research activities and science education programs supported by NSF during the fiscal year 1970 are reviewed in part one of this volume. Comprehensive listings of NSF grants and awards are presented in the second section which includes a list of…

Team Building through Wilderness Activities in Eighth Grade Special Education.

ERIC Educational Resources Information Center

West, June

The Outdoor Program is part of the special education program in a Northwest U.S. junior high school that combines wilderness activities with traditional classroom activities for students with behavior disorders. The goals of the program are to help the students learn to trust each other and the teachers, to teach students how to cooperate, and to…

49 CFR 17.6 - What procedures apply to the selection of programs and activities under these regulations?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false What procedures apply to the selection of programs and activities under these regulations? 17.6 Section 17.6 Transportation Office of the Secretary of Transportation INTERGOVERNMENTAL REVIEW OF DEPARTMENT OF TRANSPORTATION PROGRAMS AND ACTIVITIES § 17.6 What...

Development and Testing of a Nutrition, Food Safety, and Physical Activity Checklist for EFNEP and FSNE Adult Programs

ERIC Educational Resources Information Center

Bradford, Traliece; Serrano, Elena L.; Cox, Ruby H.; Lambur, Michael

2010-01-01

Objective: To develop and assess reliability and validity of the Nutrition, Food Safety, and Physical Activity Checklist to measure nutrition, food safety, and physical activity practices among adult Expanded Food and Nutrition Education Program (EFNEP) and Food Stamp Nutrition Education program (FSNE) participants. Methods: Test-retest…

Pilot-Testing CATCH Early Childhood: A Preschool-Based Healthy Nutrition and Physical Activity Program

ERIC Educational Resources Information Center

Sharma, Shreela; Chuang, Ru-Jye; Hedberg, Ann Marie

2011-01-01

Background: The literature on theoretically-based programs targeting healthy nutrition and physical activity in preschools is scarce. Purpose: To pilot test CATCH Early Childhood (CEC), a preschool-based nutrition and physical activity program among children ages three to five in Head Start. Methods: The study was conducted in two Head Start…