Preserving Envelope Efficiency in Performance Based Code Compliance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thornton, Brian A.; Sullivan, Greg P.; Rosenberg, Michael I.

2015-06-20

The City of Seattle 2012 Energy Code (Seattle 2014), one of the most progressive in the country, is under revision for its 2015 edition. Additionally, city personnel participate in the development of the next generation of the Washington State Energy Code and the International Energy Code. Seattle has pledged carbon neutrality by 2050 including buildings, transportation and other sectors. The United States Department of Energy (DOE), through Pacific Northwest National Laboratory (PNNL) provided technical assistance to Seattle in order to understand the implications of one potential direction for its code development, limiting trade-offs of long-lived building envelope components less stringentmore » than the prescriptive code envelope requirements by using better-than-code but shorter-lived lighting and heating, ventilation, and air-conditioning (HVAC) components through the total building performance modeled energy compliance path. Weaker building envelopes can permanently limit building energy performance even as lighting and HVAC components are upgraded over time, because retrofitting the envelope is less likely and more expensive. Weaker building envelopes may also increase the required size, cost and complexity of HVAC systems and may adversely affect occupant comfort. This report presents the results of this technical assistance. The use of modeled energy code compliance to trade-off envelope components with shorter-lived building components is not unique to Seattle and the lessons and possible solutions described in this report have implications for other jurisdictions and energy codes.« less

Paramyxovirus Assembly and Budding: Building Particles that Transmit Infections

PubMed Central

Harrison, Megan S.; Sakaguchi, Takemasa; Schmitt, Anthony P.

2010-01-01

The paramyxoviruses define a diverse group of enveloped RNA viruses that includes a number of important human and animal pathogens. Examples include human respiratory syncytial virus and the human parainfluenza viruses, which cause respiratory illnesses in young children and the elderly; measles and mumps viruses, which have caused recent resurgences of disease in developed countries; the zoonotic Hendra and Nipah viruses, which have caused several outbreaks of fatal disease in Australia and Asia; and Newcastle disease virus, which infects chickens and other avian species. Like other enveloped viruses, paramyxoviruses form particles that assemble and bud from cellular membranes, allowing the transmission of infections to new cells and hosts. Here, we review recent advances that have improved our understanding of events involved in paramyxovirus particle formation. Contributions of viral matrix proteins, glycoproteins, nucleocapsid proteins, and accessory proteins to particle formation are discussed, as well as the importance of host factor recruitment for efficient virus budding. Trafficking of viral structural components within infected cells is described, together with mechanisms that allow for the selection of specific sites on cellular membranes for the coalescence of viral proteins in preparation of bud formation and virion release. PMID:20398786

Optimization for energy efficiency of underground building envelope thermal performance in different climate zones of China

NASA Astrophysics Data System (ADS)

Shi, Luyang; Liu, Jing; Zhang, Huibo

2017-11-01

The object of this article is to investigate the influence of thermal performance of envelopes in shallow-buried buildings on energy consumption for different climate zones of China. For the purpose of this study, an effective building energy simulation tool (DeST) developed by Tsinghua University was chosen to model the heat transfer in underground buildings. Based on the simulative results, energy consumption for heating and cooling for the whole year was obtained. The results showed that the relationship between energy consumption and U-value of envelopes for underground buildings is different compared with above-ground buildings: improving thermal performance of exterior walls cannot reduce energy consumption, on the contrary, may result in more energy cost. Besides, it is can be derived that optimized U-values of underground building envelopes vary with climate zones of China in this study. For severe cold climate zone, the optimized U-value of underground building envelopes is 0.8W/(m2·K); for cold climate zone, the optimized U-value is 1.5W/(m2·K); for warm climate zone, the U-value is 2.0W/(m2·K).

Beyond assemblies: system convergence and multi-materiality.

PubMed

Wiscombe, Tom

2012-03-01

The architectural construction industry has become increasingly more specialized over the past 50 years, creating a culture of layer thinking over part-to-whole thinking. Building systems and technologies are often cobbled together in conflicting and uncorrelated ways, even when referred to as 'integrated', such as by way of building information modeling. True integration of building systems requires rethinking how systems and architectural morphologies can push and pull on one another, creating not only innovation in technology but in aesthetics. The revolution in composite materials, with unprecedented plasticity and performance features, opens up a huge range of possibilities for achieving this kind of convergence. Composites by nature fuse envelope and structure, but through various types of inflections, they can also be made to conduct air and fluids through cavities and de-laminations, as well as integrate lighting and energy systems. Assembly as we know it moves away from mineral materials and hardware and toward polymers and 'healing'. Further, when projected into the near-future realm of multi-materiality and 3D manufacturing, possibilities for embedding systems and creating gradients of rigidity and opacity open up, pointing to an entirely new realm of architectural thinking.

Life Cycle Assessment of Wall Systems

NASA Astrophysics Data System (ADS)

Ramachandran, Sriranjani

Natural resource depletion and environmental degradation are the stark realities of the times we live in. As awareness about these issues increases globally, industries and businesses are becoming interested in understanding and minimizing the ecological footprints of their activities. Evaluating the environmental impacts of products and processes has become a key issue, and the first step towards addressing and eventually curbing climate change. Additionally, companies are finding it beneficial and are interested in going beyond compliance using pollution prevention strategies and environmental management systems to improve their environmental performance. Life-cycle Assessment (LCA) is an evaluative method to assess the environmental impacts associated with a products' life-cycle from cradle-to-grave (i.e. from raw material extraction through to material processing, manufacturing, distribution, use, repair and maintenance, and finally, disposal or recycling). This study focuses on evaluating building envelopes on the basis of their life-cycle analysis. In order to facilitate this analysis, a small-scale office building, the University Services Building (USB), with a built-up area of 148,101 ft2 situated on ASU campus in Tempe, Arizona was studied. The building's exterior envelope is the highlight of this study. The current exterior envelope is made of tilt-up concrete construction, a type of construction in which the concrete elements are constructed horizontally and tilted up, after they are cured, using cranes and are braced until other structural elements are secured. This building envelope is compared to five other building envelope systems (i.e. concrete block, insulated concrete form, cast-in-place concrete, steel studs and curtain wall constructions) evaluating them on the basis of least environmental impact. The research methodology involved developing energy models, simulating them and generating changes in energy consumption due to the above mentioned envelope types. Energy consumption data, along with various other details, such as building floor area, areas of walls, columns, beams etc. and their material types were imported into Life-Cycle Assessment software called ATHENA impact estimator for buildings. Using this four-stepped LCA methodology, the results showed that the Steel Stud envelope performed the best and less environmental impact compared to other envelope types. This research methodology can be applied to other building typologies.

A statistical image analysis framework for pore-free islands derived from heterogeneity distribution of nuclear pore complexes.

PubMed

Mimura, Yasuhiro; Takemoto, Satoko; Tachibana, Taro; Ogawa, Yutaka; Nishimura, Masaomi; Yokota, Hideo; Imamoto, Naoko

2017-11-24

Nuclear pore complexes (NPCs) maintain cellular homeostasis by mediating nucleocytoplasmic transport. Although cyclin-dependent kinases (CDKs) regulate NPC assembly in interphase, the location of NPC assembly on the nuclear envelope is not clear. CDKs also regulate the disappearance of pore-free islands, which are nuclear envelope subdomains; this subdomain gradually disappears with increase in homogeneity of the NPC in response to CDK activity. However, a causal relationship between pore-free islands and NPC assembly remains unclear. Here, we elucidated mechanisms underlying NPC assembly from a new perspective by focusing on pore-free islands. We proposed a novel framework for image-based analysis to automatically determine the detailed 'landscape' of pore-free islands from a large quantity of images, leading to the identification of NPC intermediates that appear in pore-free islands with increased frequency in response to CDK activity. Comparison of the spatial distribution between simulated and the observed NPC intermediates within pore-free islands showed that their distribution was spatially biased. These results suggested that the disappearance of pore-free islands is highly related to de novo NPC assembly and indicated the existence of specific regulatory mechanisms for the spatial arrangement of NPC assembly on nuclear envelopes.

Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly

PubMed Central

Nguyen, Tra Huong; Leong, Daniel; Ravi, Laxmi Iyer; Tan, Boon Huan; Sandin, Sara; Sugrue, Richard J.

2017-01-01

ABSTRACT Respiratory syncytial virus (RSV) is an enveloped virus that assembles into filamentous virus particles on the surface of infected cells. Morphogenesis of RSV is dependent upon cholesterol-rich (lipid raft) membrane microdomains, but the specific role of individual raft molecules in RSV assembly is not well defined. Here, we show that RSV morphogenesis occurs within caveolar membranes and that both caveolin-1 and cavin-1 (also known as PTRF), the two major structural and functional components of caveolae, are actively recruited to and incorporated into the RSV envelope. The recruitment of caveolae occurred just prior to the initiation of RSV filament assembly, and was dependent upon an intact actin network as well as a direct physical interaction between caveolin-1 and the viral G protein. Moreover, cavin-1 protein levels were significantly increased in RSV-infected cells, leading to a virus-induced change in the stoichiometry and biophysical properties of the caveolar coat complex. Our data indicate that RSV exploits caveolae for its assembly, and we propose that the incorporation of caveolae into the virus contributes to defining the biological properties of the RSV envelope. PMID:28154158

Imaging mycobacterial growth and division with a fluorogenic probe.

PubMed

Hodges, Heather L; Brown, Robert A; Crooks, John A; Weibel, Douglas B; Kiessling, Laura L

2018-05-15

Control and manipulation of bacterial populations requires an understanding of the factors that govern growth, division, and antibiotic action. Fluorescent and chemically reactive small molecule probes of cell envelope components can visualize these processes and advance our knowledge of cell envelope biosynthesis (e.g., peptidoglycan production). Still, fundamental gaps remain in our understanding of the spatial and temporal dynamics of cell envelope assembly. Previously described reporters require steps that limit their use to static imaging. Probes that can be used for real-time imaging would advance our understanding of cell envelope construction. To this end, we synthesized a fluorogenic probe that enables continuous live cell imaging in mycobacteria and related genera. This probe reports on the mycolyltransferases that assemble the mycolic acid membrane. This peptidoglycan-anchored bilayer-like assembly functions to protect these cells from antibiotics and host defenses. Our probe, quencher-trehalose-fluorophore (QTF), is an analog of the natural mycolyltransferase substrate. Mycolyltransferases process QTF by diverting their normal transesterification activity to hydrolysis, a process that unleashes fluorescence. QTF enables high contrast continuous imaging and the visualization of mycolyltransferase activity in cells. QTF revealed that mycolyltransferase activity is augmented before cell division and localized to the septa and cell poles, especially at the old pole. This observed localization suggests that mycolyltransferases are components of extracellular cell envelope assemblies, in analogy to the intracellular divisomes and polar elongation complexes. We anticipate QTF can be exploited to detect and monitor mycobacteria in physiologically relevant environments.

Field Trial of an Aerosol-Based Enclosure Sealing Technology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harrington, Curtis; Springer, David

2015-09-01

This report presents the results from several demonstrations of a new method for sealing building envelope air leaks using an aerosol sealing process developed by the Western Cooling Efficiency Center at UC Davis. The process involves pressurizing a building while applying an aerosol sealant to the interior. As air escapes through leaks in the building envelope, the aerosol particles are transported to the leaks where they collect and form a seal that blocks the leak. Standard blower door technology is used to facilitate the building pressurization, which allows the installer to track the sealing progress during the installation and automaticallymore » verify the final building tightness. Each aerosol envelope sealing installation was performed after drywall was installed and taped, and the process did not appear to interrupt the construction schedule or interfere with other trades working in the homes. The labor needed to physically seal bulk air leaks in typical construction will not be replaced by this technology. However, this technology is capable of bringing the air leakage of a building that was built with standard construction techniques and HERS-verified sealing down to levels that would meet DOE Zero Energy Ready Homes program requirements. When a developer is striving to meet a tighter envelope leakage specification, this technology could greatly reduce the cost to achieve that goal by providing a simple and relatively low cost method for reducing the air leakage of a building envelope with little to no change in their common building practices.« less

Building America Case Study: Field Trial of an Aerosol-Based Enclosure Sealing Technology, Clovis, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

This report presents the results from several demonstrations of a new method for sealing building envelope air leaks using an aerosol sealing process developed by the Western Cooling Efficiency Center at UC Davis. The process involves pressurizing a building while applying an aerosol sealant to the interior. As air escapes through leaks in the building envelope, the aerosol particles are transported to the leaks where they collect and form a seal that blocks the leak. Standard blower door technology is used to facilitate the building pressurization, which allows the installer to track the sealing progress during the installation and automaticallymore » verify the final building tightness. Each aerosol envelope sealing installation was performed after drywall was installed and taped, and the process did not appear to interrupt the construction schedule or interfere with other trades working in the homes. The labor needed to physically seal bulk air leaks in typical construction will not be replaced by this technology. However, this technology is capable of bringing the air leakage of a building that was built with standard construction techniques and HERS-verified sealing down to levels that would meet DOE Zero Energy Ready Homes program requirements. When a developer is striving to meet a tighter envelope leakage specification, this technology could greatly reduce the cost to achieve that goal by providing a simple and relatively low cost method for reducing the air leakage of a building envelope with little to no change in their common building practices.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hun, Diana E.; Atchley, Jerald Allen; Childs, Phillip W.

Estimates for 2010 indicate that infiltration in residential buildings was responsible for 2.85 quads of energy (DOE 2014), which is about 3% of the total energy consumed in the US. One of the mechanisms being implemented to reduce this energy penalty is the use of air barriers as part of the building envelope. These technologies decrease airflow through major leakage sites such as oriented strand board (OSB) joints, and gaps around penetrations (e.g., windows, doors, pipes, electrical outlets) as indicated by Hun et al. (2014). However, most air barrier materials do not properly address leakage spots such as wall-to-roof jointsmore » and wall-to-foundation joints because these are difficult to seal, and because air barrier manufacturers usually do not provide adequate instructions for these locations. The present study focuses on characterizing typical air leakage sites in wall assemblies with air barrier materials.« less

Behavior of sandwich panels in a fire

NASA Astrophysics Data System (ADS)

Chelekova, Eugenia

2018-03-01

For the last decades there emerged a vast number of buildings and structures erected with the use of sandwich panels. The field of application for this construction material is manifold, especially in the construction of fire and explosion hazardous buildings. In advanced evacu-ation time calculation methods the coefficient of heat losses is defined with dire regard to fire load features, but without account to thermal and physical characteristics of building envelopes, or, to be exact, it is defined for brick and concrete walls with gross heat capacity. That is why the application of the heat loss coefficient expression obtained for buildings of sandwich panels is impossible because of different heat capacity of these panels from the heat capacities of brick and concrete building envelopes. The article conducts an analysis and calculation of the heal loss coefficient for buildings and structures of three layer sandwich panels as building envelopes.

Analysis for Building Envelopes and Mechanical Systems Using 2012 CBECS Data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Winiarski, David W.; Halverson, Mark A.; Butzbaugh, Joshua B.

This report describes the aggregation and mapping of certain building characteristics data available in the most recent Commercial Building Energy Consumption Survey (CBECS) (DOE EIA 2012) to describe most typical building construction practices. This report provides summary data for potential use in the support of modifications to the Pacific Northwest National Laboratory’s commercial building prototypes used for building energy code analysis. Specifically, this report outlines findings and most typical design choices for certain building envelope and heating, ventilating, and air-conditioning (HVAC) system choices.

Comparative study of air-conditioning energy use of four office buildings in China and USA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Xin; Yan, Da; An, Jingjing

Energy use in buildings has great variability. In order to design and operate low energy buildings as well as to establish building energy codes and standards and effective energy policy, it is crucial to understand and quantify key factors influencing building energy performance. Here, this study investigates air-conditioning (AC) energy use of four office buildings in four locations: Beijing, Taiwan, Hong Kong, and Berkeley. Building simulation was employed to quantify the influences of key factors, including climate, building envelope and occupant behavior. Through simulation of various combinations of the three influencing elements, it is found that climate can lead tomore » AC cooling consumption differences by almost two times, while occupant behavior resulted in the greatest differences (of up to three times) in AC cooling consumption. The influence of occupant behavior on AC energy consumption is not homogeneous. Under similar climates, when the occupant behavior in the building differed, the optimized building envelope design also differed. In conclusion, the optimal building envelope should be determined according to the climate as well as the occupants who use the building.« less

Comparative study of air-conditioning energy use of four office buildings in China and USA

DOE PAGES

Zhou, Xin; Yan, Da; An, Jingjing; ...

2018-04-05

Energy use in buildings has great variability. In order to design and operate low energy buildings as well as to establish building energy codes and standards and effective energy policy, it is crucial to understand and quantify key factors influencing building energy performance. Here, this study investigates air-conditioning (AC) energy use of four office buildings in four locations: Beijing, Taiwan, Hong Kong, and Berkeley. Building simulation was employed to quantify the influences of key factors, including climate, building envelope and occupant behavior. Through simulation of various combinations of the three influencing elements, it is found that climate can lead tomore » AC cooling consumption differences by almost two times, while occupant behavior resulted in the greatest differences (of up to three times) in AC cooling consumption. The influence of occupant behavior on AC energy consumption is not homogeneous. Under similar climates, when the occupant behavior in the building differed, the optimized building envelope design also differed. In conclusion, the optimal building envelope should be determined according to the climate as well as the occupants who use the building.« less

Evaluating wood-based composites for incipient fungal decay with the immunodiagnostic wood decay test.

Treesearch

C.A. Clausen; L. Haughton; C. Murphy

2003-01-01

Early and accurate detection of the extent of fungal deterioration during forensic inspection of the building envelope would eliminate excessive or unnecessary replacement of wood-based building materials. Areas of water infiltration in wood-framed building envelopes in the Pacific Northwest were evaluated visually and sampled for moisture content. Wood samples were...

Low-cost phase change material as an energy storage medium in building envelopes: Experimental and numerical analyses

DOE PAGES

Biswas, Kaushik; Abhari, Ramin

2014-10-03

A promising approach to increasing the energy efficiency of buildings is the implementation of a phase change material (PCM) in the building envelope. Numerous studies over the last two decades have reported the energy saving potential of PCMs in building envelopes, but their wide application has been inhibited, in part, by their high cost. This article describes a novel PCM made of naturally occurring fatty acids/glycerides trapped into high density polyethylene (HDPE) pellets and its performance in a building envelope application. The PCM-HDPE pellets were mixed with cellulose insulation and then added to an exterior wall of a test buildingmore » in a hot and humid climate, and tested over a period of several months, To demonstrate the efficacy of the PCM-enhanced cellulose insulation in reducing the building envelope heat gains and losses, side-by-side comparison was performed with another wall section filled with cellulose-only insulation. Further, numerical modeling of the test wall was performed to determine the actual impact of the PCM-HDPE pellets on wall-generated heating and cooling loads and the associated electricity consumption. The model was first validated using experimental data and then used for annual simulations using typical meteorological year (TMY3) weather data. Furthermore, this article presents the experimental data and numerical analyses showing the energy-saving potential of the new PCM.« less

Isochronic carrier-envelope phase-shift compensator.

PubMed

Görbe, Mihaly; Osvay, Karoly; Grebing, Christian; Steinmeyer, Günter

2008-11-15

A concept for orthogonal control of phase and group delay inside a laser cavity by a specially designed compensator assembly is discussed. Similar to the construction of variable polarization retarder, this assembly consists of two thin wedge prisms made from appropriately chosen optical materials. Being shifted as a whole, the assembly allows changing the phase delay with no influence on the cavity round-trip time, whereas relative shifting of the prisms enables adjustment of the latter. This scheme is discussed theoretically and verified experimentally, indicating a factor 30 reduction of the influence on the repetition rate compared to the commonly used silica wedge pair. For a 2pi adjustment of the carrier-envelope phase shift, single-pass timing differences are reduced to the single-femtosecond regime. With negligible distortions of timing and dispersion, the described compensator device greatly simplifies carrier-envelope phase control and experiments in extreme nonlinear optics. Copyright (c) 2008 Optical Society of America.

Apolipoprotein E Likely Contributes to a Maturation Step of Infectious Hepatitis C Virus Particles and Interacts with Viral Envelope Glycoproteins

PubMed Central

Lee, Ji-Young; Acosta, Eliana G.; Stoeck, Ina Karen; Long, Gang; Hiet, Marie-Sophie; Mueller, Birthe; Fackler, Oliver T.; Kallis, Stephanie

2014-01-01

ABSTRACT The assembly of infectious hepatitis C virus (HCV) particles is tightly linked to components of the very-low-density lipoprotein (VLDL) pathway. We and others have shown that apolipoprotein E (ApoE) plays a major role in production of infectious HCV particles. However, the mechanism by which ApoE contributes to virion assembly/release and how it gets associated with the HCV particle is poorly understood. We found that knockdown of ApoE reduces titers of infectious intra- and extracellular HCV but not of the related dengue virus. ApoE depletion also reduced amounts of extracellular HCV core protein without affecting intracellular core amounts. Moreover, we found that ApoE depletion affected neither formation of nucleocapsids nor their envelopment, suggesting that ApoE acts at a late step of assembly, such as particle maturation and infectivity. Importantly, we demonstrate that ApoE interacts with the HCV envelope glycoproteins, most notably E2. This interaction did not require any other viral proteins and depended on the transmembrane domain of E2 that also was required for recruitment of HCV envelope glycoproteins to detergent-resistant membrane fractions. These results suggest that ApoE plays an important role in HCV particle maturation, presumably by direct interaction with viral envelope glycoproteins. IMPORTANCE The HCV replication cycle is tightly linked to host cell lipid pathways and components. This is best illustrated by the dependency of HCV assembly on lipid droplets and the VLDL component ApoE. Although the role of ApoE for production of infectious HCV particles is well established, it is still poorly understood how ApoE contributes to virion formation and how it gets associated with HCV particles. Here, we provide experimental evidence that ApoE likely is required for an intracellular maturation step of HCV particles. Moreover, we demonstrate that ApoE associates with the viral envelope glycoproteins. This interaction appears to be dispensable for envelopment of virus particles but likely contributes to the quality control of secreted infectious virions. These results shed new light on the exploitation of host cell lipid pathways by HCV and the link of viral particle assembly to the VLDL component ApoE. PMID:25122793

Coordination of Hepatitis C Virus Assembly by Distinct Regulatory Regions in Nonstructural Protein 5A

PubMed Central

Zayas, Margarita; Long, Gang; Madan, Vanesa; Bartenschlager, Ralf

2016-01-01

Hepatitis C virus (HCV) nonstructural protein (NS)5A is a RNA-binding protein composed of a N-terminal membrane anchor, a structured domain I (DI) and two intrinsically disordered domains (DII and DIII) interacting with viral and cellular proteins. While DI and DII are essential for RNA replication, DIII is required for assembly. How these processes are orchestrated by NS5A is poorly understood. In this study, we identified a highly conserved basic cluster (BC) at the N-terminus of DIII that is critical for particle assembly. We generated BC mutants and compared them with mutants that are blocked at different stages of the assembly process: a NS5A serine cluster (SC) mutant blocked in NS5A-core interaction and a mutant lacking the envelope glycoproteins (ΔE1E2). We found that BC mutations did not affect core-NS5A interaction, but strongly impaired core–RNA association as well as virus particle envelopment. Moreover, BC mutations impaired RNA-NS5A interaction arguing that the BC might be required for loading of core protein with viral RNA. Interestingly, RNA-core interaction was also reduced with the ΔE1E2 mutant, suggesting that nucleocapsid formation and envelopment are coupled. These findings argue for two NS5A DIII determinants regulating assembly at distinct, but closely linked steps: (i) SC-dependent recruitment of replication complexes to core protein and (ii) BC-dependent RNA genome delivery to core protein, triggering encapsidation that is tightly coupled to particle envelopment. These results provide a striking example how a single viral protein exerts multiple functions to coordinate the steps from RNA replication to the assembly of infectious virus particles. PMID:26727512

NSF- and SNARE-mediated membrane fusion is required for nuclear envelope formation and completion of nuclear pore complex assembly in Xenopus laevis egg extracts.

PubMed

Baur, Tina; Ramadan, Kristijan; Schlundt, Andreas; Kartenbeck, Jürgen; Meyer, Hemmo H

2007-08-15

Despite the progress in understanding nuclear envelope (NE) reformation after mitosis, it has remained unclear what drives the required membrane fusion and how exactly this is coordinated with nuclear pore complex (NPC) assembly. Here, we show that, like other intracellular fusion reactions, NE fusion in Xenopus laevis egg extracts is mediated by SNARE proteins that require activation by NSF. Antibodies against Xenopus NSF, depletion of NSF or the dominant-negative NSF(E329Q) variant specifically inhibited NE formation. Staging experiments further revealed that NSF was required until sealing of the envelope was completed. Moreover, excess exogenous alpha-SNAP that blocks SNARE function prevented membrane fusion and caused accumulation of non-flattened vesicles on the chromatin surface. Under these conditions, the nucleoporins Nup107 and gp210 were fully recruited, whereas assembly of FxFG-repeat-containing nucleoporins was blocked. Together, we define NSF- and SNARE-mediated membrane fusion events as essential steps during NE formation downstream of Nup107 recruitment, and upstream of membrane flattening and completion of NPC assembly.

Weather Correlations to Calculate Infiltration Rates for U. S. Commercial Building Energy Models.

PubMed

Ng, Lisa C; Quiles, Nelson Ojeda; Dols, W Stuart; Emmerich, Steven J

2018-01-01

As building envelope performance improves, a greater percentage of building energy loss will occur through envelope leakage. Although the energy impacts of infiltration on building energy use can be significant, current energy simulation software have limited ability to accurately account for envelope infiltration and the impacts of improved airtightness. This paper extends previous work by the National Institute of Standards and Technology that developed a set of EnergyPlus inputs for modeling infiltration in several commercial reference buildings using Chicago weather. The current work includes cities in seven additional climate zones and uses the updated versions of the prototype commercial building types developed by the Pacific Northwest National Laboratory for the U. S. Department of Energy. Comparisons were made between the predicted infiltration rates using three representations of the commercial building types: PNNL EnergyPlus models, CONTAM models, and EnergyPlus models using the infiltration inputs developed in this paper. The newly developed infiltration inputs in EnergyPlus yielded average annual increases of 3 % and 8 % in the HVAC electrical and gas use, respectively, over the original infiltration inputs in the PNNL EnergyPlus models. When analyzing the benefits of building envelope airtightening, greater HVAC energy savings were predicted using the newly developed infiltration inputs in EnergyPlus compared with using the original infiltration inputs. These results indicate that the effects of infiltration on HVAC energy use can be significant and that infiltration can and should be better accounted for in whole-building energy models.

Interior thermal insulation systems for historical building envelopes

NASA Astrophysics Data System (ADS)

Jerman, Miloš; Solař, Miloš; Černý, Robert

2017-11-01

The design specifics of interior thermal insulation systems applied for historical building envelopes are described. The vapor-tight systems and systems based on capillary thermal insulation materials are taken into account as two basic options differing in building-physical considerations. The possibilities of hygrothermal analysis of renovated historical envelopes including laboratory methods, computer simulation techniques, and in-situ tests are discussed. It is concluded that the application of computational models for hygrothermal assessment of interior thermal insulation systems should always be performed with a particular care. On one hand, they present a very effective tool for both service life assessment and possible planning of subsequent reconstructions. On the other, the hygrothermal analysis of any historical building can involve quite a few potential uncertainties which may affect negatively the accuracy of obtained results.

10 CFR 434.516 - Building exterior envelope.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Proposed Design, the envelope characteristics of the Proposed Design shall be used. 516.2 Infiltration. For Prototype and Reference Buildings, the infiltration assumptions in subsection 516.2.1 shall be prescribed.... Infiltration shall impact perimeter zones only. 516.2.1 When the HVAC system is switched “on,” no infiltration...

Thermal Insulating Concrete Wall Panel Design for Sustainable Built Environment

PubMed Central

Zhou, Ao; Wong, Kwun-Wah

2014-01-01

Air-conditioning system plays a significant role in providing users a thermally comfortable indoor environment, which is a necessity in modern buildings. In order to save the vast energy consumed by air-conditioning system, the building envelopes in envelope-load dominated buildings should be well designed such that the unwanted heat gain and loss with environment can be minimized. In this paper, a new design of concrete wall panel that enhances thermal insulation of buildings by adding a gypsum layer inside concrete is presented. Experiments have been conducted for monitoring the temperature variation in both proposed sandwich wall panel and conventional concrete wall panel under a heat radiation source. For further understanding the thermal effect of such sandwich wall panel design from building scale, two three-story building models adopting different wall panel designs are constructed for evaluating the temperature distribution of entire buildings using finite element method. Both the experimental and simulation results have shown that the gypsum layer improves the thermal insulation performance by retarding the heat transfer across the building envelopes. PMID:25177718

Thermal insulating concrete wall panel design for sustainable built environment.

PubMed

Zhou, Ao; Wong, Kwun-Wah; Lau, Denvid

2014-01-01

Air-conditioning system plays a significant role in providing users a thermally comfortable indoor environment, which is a necessity in modern buildings. In order to save the vast energy consumed by air-conditioning system, the building envelopes in envelope-load dominated buildings should be well designed such that the unwanted heat gain and loss with environment can be minimized. In this paper, a new design of concrete wall panel that enhances thermal insulation of buildings by adding a gypsum layer inside concrete is presented. Experiments have been conducted for monitoring the temperature variation in both proposed sandwich wall panel and conventional concrete wall panel under a heat radiation source. For further understanding the thermal effect of such sandwich wall panel design from building scale, two three-story building models adopting different wall panel designs are constructed for evaluating the temperature distribution of entire buildings using finite element method. Both the experimental and simulation results have shown that the gypsum layer improves the thermal insulation performance by retarding the heat transfer across the building envelopes.

Breach of the nuclear lamina during assembly of herpes simplex viruses.

PubMed

Morrison, Lynda A; DeLassus, Gregory S

2011-01-01

Beneath the inner nuclear membrane lies the dense meshwork of the nuclear lamina, which provides structural support for the nuclear envelope and serves as an important organizing center for a number of nuclear and cytoplasmic constituents and processes. Herpesviruses have a significant and wide-ranging impact on human health, and their capacity to replicate and cause disease includes events that occur in the host cell nucleus. Herpesviruses begin assembly of progeny virus in the nuclei of infected cells and their capsids must escape the confines of the nucleus by budding through the inner nuclear membrane (INM) to proceed with later stages of virion assembly and egress. Access of viral capsids to the INM thus necessitates disruption of the dense nuclear lamina layer. We review herpesvirus effects on the nuclear lamina and in particular the roles of the herpes simplex virus-encoded nuclear envelope complex and viral kinases on lamin phosphorylation, dissociation, and nucleocapsid envelopment at the INM.

The assembly of stellar haloes in massive Early-Type Galaxies

NASA Astrophysics Data System (ADS)

Buitrago, F.

2017-03-01

Massive (Mstellar >= 5×1010 M⊙) Early-Type Galaxies (ETGs) must build an outer stellar envelope over cosmic time in order to account for their remarkable size evolution. This is similar to what occurs to nearby Late-Type Galaxies (LTGs), which create their stellar haloes out of the debris of lower mass systems. We analysed the outer parts of massive ETGs at z < 1 by exploiting the Hubble Ultra Deep Field imaging. These galaxies store 10-30% of their stellar mass at distances 10 < R/kpc < 50, in contrast to the low percentages (< 5%) found for LTGs. We find evidence for a progressive outskirt development with redshift driven solely via merging.

Structure of a Venezuelan equine encephalitis virus assembly intermediate isolated from infected cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lamb, Kristen; Lokesh, G.L.; Sherman, Michael

2010-10-25

Venezuelan equine encephalitis virus (VEEV) is a prototypical enveloped ssRNA virus of the family Togaviridae. To better understand alphavirus assembly, we analyzed newly formed nucleocapsid particles (termed pre-viral nucleocapsids) isolated from infected cells. These particles were intermediates along the virus assembly pathway, and ultimately bind membrane-associated viral glycoproteins to bud as mature infectious virus. Purified pre-viral nucleocapsids were spherical with a unimodal diameter distribution. The structure of one class of pre-viral nucleocapsids was determined with single particle reconstruction of cryo-electron microscopy images. These studies showed that pre-viral nucleocapsids assembled into an icosahedral structure with a capsid stoichiometry similar to themore » mature nucleocapsid. However, the individual capsomers were organized significantly differently within the pre-viral and mature nucleocapsids. The pre-viral nucleocapsid structure implies that nucleocapsids are highly plastic and undergo glycoprotein and/or lipid-driven rearrangements during virus self-assembly. This mechanism of self-assembly may be general for other enveloped viruses.« less

Design tools

Treesearch

Anton TenWolde; Mark T. Bomberg

2009-01-01

Overall, despite the lack of exact input data, the use of design tools, including models, is much superior to the simple following of rules of thumbs, and a moisture analysis should be standard procedure for any building envelope design. Exceptions can only be made for buildings in the same climate, similar occupancy, and similar envelope construction. This chapter...

Assembly of viral particles in Xenopus oocytes: pre-surface-antigens regulate secretion of the hepatitis B viral surface envelope particle.

PubMed Central

Standring, D N; Ou, J H; Rutter, W J

1986-01-01

Infection with hepatitis B virus (HBV) is associated with the production of a viral envelope particle that contains membrane lipids, surface antigen (S), and two presurface-antigens (pre-S) comprised of the entire S moiety with approximately 55 (pre-S2) and 174 (pre-S1) additional NH2-terminal amino acids. We show here that Xenopus oocytes injected with synthetic S mRNA assemble and secrete characteristic 22-nm viral envelope particles. In contrast, pre-S1 and pre-S2 antigens are synthesized but not secreted. By coinjecting mRNAs, we found that synthesis of high levels of pre-S proteins specifically inhibits S antigen secretion. On the other hand, high levels of S synthesis can drive the secretion of small amounts of either pre-S antigen. These observations are consistent with a model for viral envelope assembly in which both S and pre-S proteins are incorporated into a multimeric particle, presumably via interactions between the S protein domains, while the pre-S amino-terminal moieties regulate the secretion of this structure. Our results indicate that Xenopus oocytes will provide a powerful system for studying the morphogenesis of simple structures of viral or cellular origin. Images PMID:3467308

14 CFR Appendix A to Part 31 - Instructions for Continued Airworthiness

Code of Federal Regulations, 2011 CFR

2011-01-01

... that covers details regarding servicing of balloon components, including burner nozzles, fuel tanks... envelope, controls, rigging, basket structure, fuel systems, instruments, and heater assembly that provides... preparation including any storage limits. (i) Instructions for repair on the balloon envelope and its basket...

14 CFR Appendix A to Part 31 - Instructions for Continued Airworthiness

Code of Federal Regulations, 2012 CFR

2012-01-01

... that covers details regarding servicing of balloon components, including burner nozzles, fuel tanks... envelope, controls, rigging, basket structure, fuel systems, instruments, and heater assembly that provides... preparation including any storage limits. (i) Instructions for repair on the balloon envelope and its basket...

14 CFR Appendix A to Part 31 - Instructions for Continued Airworthiness

Code of Federal Regulations, 2014 CFR

2014-01-01

... that covers details regarding servicing of balloon components, including burner nozzles, fuel tanks... envelope, controls, rigging, basket structure, fuel systems, instruments, and heater assembly that provides... preparation including any storage limits. (i) Instructions for repair on the balloon envelope and its basket...

14 CFR Appendix A to Part 31 - Instructions for Continued Airworthiness

Code of Federal Regulations, 2013 CFR

2013-01-01

... that covers details regarding servicing of balloon components, including burner nozzles, fuel tanks... envelope, controls, rigging, basket structure, fuel systems, instruments, and heater assembly that provides... preparation including any storage limits. (i) Instructions for repair on the balloon envelope and its basket...

Hygrothermal Simulation: A Tool for Building Envelope Design Analysis

Treesearch

Samuel V. Glass; Anton TenWolde; Samuel L. Zelinka

2013-01-01

Is it possible to gauge the risk of moisture problems while designing the building envelope? This article provides a brief introduction to computer-based hygrothermal (heat and moisture) simulation, shows how simulation can be useful as a design tool, and points out a number of im-portant considerations regarding model inputs and limita-tions. Hygrothermal simulation...

Complex analysis of energy efficiency in operated high-rise residential building: Case study

NASA Astrophysics Data System (ADS)

Korniyenko, Sergey

2018-03-01

Energy conservation and human thermal comfort enhancement in buildings is a topical issue of modern architecture and construction. The innovative solution of this problem makes it possible to enhance building ecological and maintenance safety, to reduce hydrocarbon fuel consumption, and to improve life standard of people. The requirements to increase of energy efficiency in buildings should be provided at all the stages of building's life cycle that is at the stage of design, construction and maintenance of buildings. The research purpose is complex analysis of energy efficiency in operated high-rise residential building. Many actions for building energy efficiency are realized according to the project; mainly it is the effective building envelope and engineering systems. Based on results of measurements the energy indicators of the building during annual period have been calculated. The main reason of increase in heat losses consists in the raised infiltration of external air in the building through a building envelope owing to the increased air permeability of windows and balcony doors (construction defects). Thermorenovation of the building based on ventilating and infiltration heat losses reduction through a building envelope allows reducing annual energy consumption. Energy efficiency assessment based on the total annual energy consumption of building, including energy indices for heating and a ventilation, hot water supply and electricity supply, in comparison with heating is more complete. The account of various components in building energy balance completely corresponds to modern direction of researches on energy conservation and thermal comfort enhancement in buildings.

Field Trial of an Aerosol-Based Enclosure Sealing Technology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harrington, Curtis; Springer, David

2015-09-01

This report presents the results from several demonstrations of a new method for sealing building envelope air leaks using an aerosol sealing process developed by the Western Cooling Efficiency Center at UC Davis. The process involves pressurizing a building while applying an aerosol sealant to the interior. As air escapes through leaks in the building envelope, the aerosol particles are transported to the leaks where they collect and form a seal that blocks the leak. Standard blower door technology is used to facilitate the building pressurization, which allows the installer to track the sealing progress during the installation and automaticallymore » verify the final building tightness. Each aerosol envelope sealing installation was performed after drywall was installed and taped, and the process did not appear to interrupt the construction schedule or interfere with other trades working in the homes. The labor needed to physically seal bulk air leaks in typical construction will not be replaced by this technology.« less

Endogenous avian leukosis viral loci in the Red Jungle Fowl genome assembly.

PubMed

Benkel, Bernhard; Rutherford, Katherine

2014-12-01

The current build (galGal4) of the genome of the ancestor of the modern chicken, the Red Jungle Fowl, contains a single endogenous avian leukosis viral element (ALVE) on chromosome 1 (designated RSV-LTR; family ERVK). The assembly shows the ALVE provirus juxtaposed with a member of a second family of avian endogenous retroviruses (designated GGERV20; family ERVL); however, the status of the 3' end of the ALVE element as well as its flanking region remain unclear due to a gap in the reference genome sequence. In this study, we filled the gap in the assembly using a combination of long-range PCR (LR-PCR) and a short contig present in the unassembled portion of the reference genome database. Our results demonstrate that the ALVE element (ALVE-JFevB) is inserted into the putative envelope region of a GGERV20 element, roughly 1 kbp from its 3' end, and that ALVE-JFevB is complete, and depending on its expression status, potentially capable of directing the production of virus. Moreover, the unassembled portion of the genome database contains junction fragments for a second, previously characterized endogenous proviral element, ALVE-6. ©2014 Poultry Science Association Inc.

To Investigate the Influence of Building Envelope and Natural Ventilation on Thermal Heat Balance in Office Buildings in Warm and Humid Climate

NASA Astrophysics Data System (ADS)

Kini, Pradeep G.; Garg, Naresh Kumar; Kamath, Kiran

2017-07-01

India’s commercial building sector is witnessing robust growth. India continues to be a key growth market among global corporates and this is reflective in the steady growth in demand for prime office space. A recent trend that has been noted is the increase in demand for office spaces not just in major cities but also in smaller tier II and Tier III cities. Growth in the commercial building sector projects a rising trend of energy intensive mechanical systems in office buildings in India. The air conditioning market in India is growing at 25% annually. This is due to the ever increasing demand to maintain thermal comfort in tropical regions. Air conditioning is one of the most energy intensive technologies which are used in buildings. As a result India is witnessing significant spike in energy demand and further widening the demand supply gap. Challenge in India is to identify passive measures in building envelope design in office buildings to reduce the cooling loads and conserve energy. This paper investigates the overall heat gain through building envelope components and natural ventilation in warm and humid climate region through experimental and simulation methods towards improved thermal environmental performance.

Analyzing the effect of the longwave emissivity and solar reflectance of building envelopes on energy-saving in buildings in various climates

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shi, Zhiyang; Zhang, Xiong

A dynamic computer simulation is carried out in the climates of 35 cities distributed around the world. The variation of the annual air-conditioning energy loads due to changes in the longwave emissivity and the solar reflectance of the building envelopes is studied to find the most appropriate exterior building finishes in various climates (including a tropical climate, a subtropical climate, a mountain plateau climate, a frigid-temperate climate and a temperate climate). Both the longwave emissivity and the solar reflectance are set from 0.1 to 0.9 with an interval of 0.1 in the simulation. The annual air-conditioning energy loads trends ofmore » each city are listed in a chart. The results show that both the longwave emissivity and the solar reflectance of building envelopes play significant roles in energy-saving for buildings. In tropical climates, the optical parameters of the building exterior surface affect the building energy-saving most significantly. In the mountain plateau climates and the subarctic climates, the impacts on energy-saving in buildings due to changes in the longwave emissivity and the solar reflectance are still considerable, but in the temperate continental climates and the temperate maritime climates, only limited effects are seen. (author)« less

Low-Cost Bio-Based Phase Change Materials as an Energy Storage Medium in Building Envelopes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Biswas, Kaushik; Abhari, Mr. Ramin; Shukla, Dr. Nitin

2015-01-01

A promising approach to increasing the energy efficiency of buildings is the implementation of phase change material (PCM) in building envelope systems. Several studies have reported the energy saving potential of PCM in building envelopes. However, wide application of PCMs in building applications has been inhibited, in part, by their high cost. This article describes a novel paraffin product made of naturally occurring fatty acids/glycerides trapped into high density polyethylene (HDPE) pellets and its performance in a building envelope application, with the ultimate goal of commercializing a low-cost PCM platform. The low-cost PCM pellets were mixed with cellulose insulation, installedmore » in external walls and field-tested under natural weatherization conditions for a period of several months. In addition, several PCM samples and PCM-cellulose samples were prepared under controlled conditions for laboratory-scale testing. The laboratory tests were performed to determine the phase change properties of PCM-enhanced cellulose insulation both at microscopic and macroscopic levels. This article presents the data and analysis from the exterior test wall and the laboratory-scale test data. PCM behavior is influenced by the weather and interior conditions, PCM phase change temperature and PCM distribution within the wall cavity, among other factors. Under optimal conditions, the field data showed up to 20% reduction in weekly heat transfer through an external wall due to the PCM compared to cellulose-only insulation.« less

Intracellular self-assembly based multi-labeling of key viral components: Envelope, capsid and nucleic acids.

PubMed

Wen, Li; Lin, Yi; Zhang, Zhi-Ling; Lu, Wen; Lv, Cheng; Chen, Zhi-Liang; Wang, Han-Zhong; Pang, Dai-Wen

2016-08-01

Envelope, capsid and nucleic acids are key viral components that are all involved in crucial events during virus infection. Thus simultaneous labeling of these key components is an indispensable prerequisite for monitoring comprehensive virus infection process and dissecting virus infection mechanism. Baculovirus was genetically tagged with biotin on its envelope protein GP64 and enhanced green fluorescent protein (EGFP) on its capsid protein VP39. Spodoptera frugiperda 9 (Sf9) cells were infected by the recombinant baculovirus and subsequently fed with streptavidin-conjugated quantum dots (SA-QDs) and cell-permeable nucleic acids dye SYTO 82. Just by genetic engineering and virus propagation, multi-labeling of envelope, capsid and nucleic acids was spontaneously accomplished during virus inherent self-assembly process, significantly simplifying the labeling process while maintaining virus infectivity. Intracellular dissociation and transportation of all the key viral components, which was barely reported previously, was real-time monitored based on the multi-labeling approach, offering opportunities for deeply understanding virus infection and developing anti-virus treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

Role of Gag and lipids during HIV-1 assembly in CD4+ T cells and macrophages

PubMed Central

Mariani, Charlotte; Desdouits, Marion; Favard, Cyril; Benaroch, Philippe; Muriaux, Delphine M.

2014-01-01

HIV-1 is an RNA enveloped virus that preferentially infects CD4+ T lymphocytes and also macrophages. In CD4+ T cells, HIV-1 mainly buds from the host cell plasma membrane. The viral Gag polyprotein targets the plasma membrane and is the orchestrator of the HIV assembly as its expression is sufficient to promote the formation of virus-like particles carrying a lipidic envelope derived from the host cell membrane. Certain lipids are enriched in the viral membrane and are thought to play a key role in the assembly process and the envelop composition. A large body of work performed on infected CD4+ T cells has provided important knowledge about the assembly process and the membrane virus lipid composition. While HIV assembly and budding in macrophages is thought to follow the same general Gag-driven mechanism as in T-lymphocytes, the HIV cycle in macrophage exhibits specific features. In these cells, new virions bud from the limiting membrane of seemingly intracellular compartments, where they accumulate while remaining infectious. These structures are now often referred to as Virus Containing Compartments (VCCs). Recent studies suggest that VCCs represent intracellularly sequestered regions of the plasma membrane, but their precise nature remains elusive. The proteomic and lipidomic characterization of virions produced by T cells or macrophages has highlighted the similarity between their composition and that of the plasma membrane of producer cells, as well as their enrichment in acidic lipids, some components of raft lipids and in tetraspanin-enriched microdomains. It is likely that Gag promotes the coalescence of these components into an assembly platform from which viral budding takes place. How Gag exactly interacts with membrane lipids and what are the mechanisms involved in the interaction between the different membrane nanodomains within the assembly platform remains unclear. Here we review recent literature regarding the role of Gag and lipids on HIV-1 assembly in CD4+ T cells and macrophages. PMID:25009540

Biomimetic interfaces based on S-layer proteins, lipid membranes and functional biomolecules

PubMed Central

Schuster, Bernhard; Sleytr, Uwe B.

2014-01-01

Designing and utilization of biomimetic membrane systems generated by bottom-up processes is a rapidly growing scientific and engineering field. Elucidation of the supramolecular construction principle of archaeal cell envelopes composed of S-layer stabilized lipid membranes led to new strategies for generating highly stable functional lipid membranes at meso- and macroscopic scale. In this review, we provide a state-of-the-art survey of how S-layer proteins, lipids and polymers may be used as basic building blocks for the assembly of S-layer-supported lipid membranes. These biomimetic membrane systems are distinguished by a nanopatterned fluidity, enhanced stability and longevity and, thus, provide a dedicated reconstitution matrix for membrane-active peptides and transmembrane proteins. Exciting areas in the (lab-on-a-) biochip technology are combining composite S-layer membrane systems involving specific membrane functions with the silicon world. Thus, it might become possible to create artificial noses or tongues, where many receptor proteins have to be exposed and read out simultaneously. Moreover, S-layer-coated liposomes and emulsomes copying virus envelopes constitute promising nanoformulations for the production of novel targeting, delivery, encapsulation and imaging systems. PMID:24812051

Targeting and assembly of components of the TOC protein import complex at the chloroplast outer envelope membrane

PubMed Central

Richardson, Lynn G. L.; Paila, Yamuna D.; Siman, Steven R.; Chen, Yi; Smith, Matthew D.; Schnell, Danny J.

2014-01-01

The translocon at the outer envelope membrane of chloroplasts (TOC) initiates the import of thousands of nuclear encoded preproteins required for chloroplast biogenesis and function. The multimeric TOC complex contains two GTP-regulated receptors, Toc34 and Toc159, which recognize the transit peptides of preproteins and initiate protein import through a β–barrel membrane channel, Toc75. Different isoforms of Toc34 and Toc159 assemble with Toc75 to form structurally and functionally diverse translocons, and the composition and levels of TOC translocons is required for the import of specific subsets of coordinately expressed proteins during plant growth and development. Consequently, the proper assembly of the TOC complexes is key to ensuring organelle homeostasis. This review will focus on our current knowledge of the targeting and assembly of TOC components to form functional translocons at the outer membrane. Our analyses reveal that the targeting of TOC components involves elements common to the targeting of other outer membrane proteins, but also include unique features that appear to have evolved to specifically facilitate assembly of the import apparatus. PMID:24966864

Targeting and assembly of components of the TOC protein import complex at the chloroplast outer envelope membrane.

PubMed

Richardson, Lynn G L; Paila, Yamuna D; Siman, Steven R; Chen, Yi; Smith, Matthew D; Schnell, Danny J

2014-01-01

The translocon at the outer envelope membrane of chloroplasts (TOC) initiates the import of thousands of nuclear encoded preproteins required for chloroplast biogenesis and function. The multimeric TOC complex contains two GTP-regulated receptors, Toc34 and Toc159, which recognize the transit peptides of preproteins and initiate protein import through a β-barrel membrane channel, Toc75. Different isoforms of Toc34 and Toc159 assemble with Toc75 to form structurally and functionally diverse translocons, and the composition and levels of TOC translocons is required for the import of specific subsets of coordinately expressed proteins during plant growth and development. Consequently, the proper assembly of the TOC complexes is key to ensuring organelle homeostasis. This review will focus on our current knowledge of the targeting and assembly of TOC components to form functional translocons at the outer membrane. Our analyses reveal that the targeting of TOC components involves elements common to the targeting of other outer membrane proteins, but also include unique features that appear to have evolved to specifically facilitate assembly of the import apparatus.

Isolating The Building Thermal Envelope

NASA Astrophysics Data System (ADS)

Harrje, D. T.; Dutt, G. S.; Gadsby, K. J.

1981-01-01

The evaluation of the thermal integrity of building envelopes by infrared scanning tech-niques is often hampered in mild weather because temperature differentials across the envelope are small. Combining the infrared scanning with positive or negative building pressures, induced by a "blower door" or the building ventilation system, considerably extends the periods during which meaningful diagnostics can be conducted. Although missing or poorly installed insulation may lead to a substantial energy penalty, it is the search for air leakage sites that often has the largest potential for energy savings. Infrared inspection of the attic floor with air forced from the occupied space through ceiling by-passes, and inspecting the interior of the building when outside air is being sucked through the envelope reveals unexpected leakage sites. Portability of the diagnostic equipment is essential in these surveys which may include access into some tight spaces. A catalog of bypass heat losses that have been detected in residential housing using the combined infrared pressure differential technique is included to point out the wide variety of leakage sites which may compromise the benefits of thermal insulation and allow excessive air infiltration. Detection and suppression of such leaks should be key items in any building energy audit program. Where a calibrated blower door is used to pressurize or evacuate the house, the leakage rate can be quantified and an excessively tight house recognized. Houses that are too tight may be improved with a minimal energy penalty by forced ventilation,preferably with a heat recuperator and/or by providing combustion air directly to the furnace.

Arabidopsis ARC6 coordinates the division machineries of the inner and outer chloroplast membranes through interaction with PDV2 in the intermembrane space.

PubMed

Glynn, Jonathan M; Froehlich, John E; Osteryoung, Katherine W

2008-09-01

Chloroplasts arose from a free-living cyanobacterial endosymbiont and divide by binary fission. Division involves the assembly and constriction of the endosymbiont-derived, tubulin-like FtsZ ring on the stromal surface of the inner envelope membrane and the host-derived, dynamin-like ARC5 ring on the cytosolic surface of the outer envelope membrane. Despite the identification of many proteins required for plastid division, the factors coordinating the internal and external division machineries are unknown. Here, we provide evidence that this coordination is mediated in Arabidopsis thaliana by an interaction between ARC6, an FtsZ assembly factor spanning the inner envelope membrane, and PDV2, an ARC5 recruitment factor spanning the outer envelope membrane. ARC6 and PDV2 interact via their C-terminal domains in the intermembrane space, consistent with their in vivo topologies. ARC6 acts upstream of PDV2 to localize PDV2 (and hence ARC5) to the division site. We present a model whereby ARC6 relays information on stromal FtsZ ring positioning through PDV2 to the chloroplast surface to specify the site of ARC5 recruitment. Because orthologs of ARC6 occur in land plants, green algae, and cyanobacteria but PDV2 occurs only in land plants, the connection between ARC6 and PDV2 represents the evolution of a plant-specific adaptation to coordinate the assembly and activity of the endosymbiont- and host-derived plastid division components.

Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope

PubMed Central

Otsuka, Shotaro; Bui, Khanh Huy; Schorb, Martin; Hossain, M Julius; Politi, Antonio Z; Koch, Birgit; Eltsov, Mikhail; Beck, Martin; Ellenberg, Jan

2016-01-01

The nuclear pore complex (NPC) mediates nucleocytoplasmic transport through the nuclear envelope. How the NPC assembles into this double membrane boundary has remained enigmatic. Here, we captured temporally staged assembly intermediates by correlating live cell imaging with high-resolution electron tomography and super-resolution microscopy. Intermediates were dome-shaped evaginations of the inner nuclear membrane (INM), that grew in diameter and depth until they fused with the flat outer nuclear membrane. Live and super-resolved fluorescence microscopy revealed the molecular maturation of the intermediates, which initially contained the nuclear and cytoplasmic ring component Nup107, and only later the cytoplasmic filament component Nup358. EM particle averaging showed that the evagination base was surrounded by an 8-fold rotationally symmetric ring structure from the beginning and that a growing mushroom-shaped density was continuously associated with the deforming membrane. Quantitative structural analysis revealed that interphase NPC assembly proceeds by an asymmetric inside-out extrusion of the INM. DOI: http://dx.doi.org/10.7554/eLife.19071.001 PMID:27630123

HIV-1 Envelope Glycoprotein Trafficking through the Endosomal Recycling Compartment Is Required for Particle Incorporation.

PubMed

Kirschman, Junghwa; Qi, Mingli; Ding, Lingmei; Hammonds, Jason; Dienger-Stambaugh, Krista; Wang, Jaang-Jiun; Lapierre, Lynne A; Goldenring, James R; Spearman, Paul

2018-03-01

The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) encodes specific trafficking signals within its long cytoplasmic tail (CT) that regulate incorporation into HIV-1 particles. Rab11-family interacting protein 1C (FIP1C) and Rab14 are host trafficking factors required for Env particle incorporation, suggesting that Env undergoes sorting from the endosomal recycling compartment (ERC) to the site of particle assembly on the plasma membrane. We disrupted outward sorting from the ERC by expressing a C-terminal fragment of FIP1C (FIP1C 560-649 ) and examined the consequences on Env trafficking and incorporation into particles. FIP1C 560-649 reduced cell surface levels of Env and prevented its incorporation into HIV-1 particles. Remarkably, Env was trapped in an exaggerated perinuclear ERC in a CT-dependent manner. Mutation of either the Yxxϕ endocytic motif or the YW 795 motif in the CT prevented Env trapping in the ERC and restored incorporation into particles. In contrast, simian immunodeficiency virus SIVmac239 Env was not retained in the ERC, while substitution of the HIV-1 CT for the SIV CT resulted in SIV Env retention in this compartment. These results provide the first direct evidence that Env traffics through the ERC and support a model whereby HIV-1 Env is specifically targeted to the ERC prior to FIP1C- and CT-dependent outward sorting to the particle assembly site on the plasma membrane. IMPORTANCE The HIV envelope protein is an essential component of the viral particle. While many aspects of envelope protein structure and function have been established, the pathway it follows in the cell prior to reaching the site of particle assembly is not well understood. The envelope protein has a very long cytoplasmic tail that interacts with the host cell trafficking machinery. Here, we utilized a truncated form of the trafficking adaptor FIP1C protein to arrest the intracellular transport of the envelope protein, demonstrating that it becomes trapped inside the cell within the endosomal recycling compartment. Intracellular trapping resulted in a loss of envelope protein on released particles and a corresponding loss of infectivity. Mutations of specific trafficking motifs in the envelope protein tail prevented its trapping in the recycling compartment. These results establish that trafficking to the endosomal recycling compartment is an essential step in HIV envelope protein particle incorporation. Copyright © 2018 American Society for Microbiology.

Composite S-layer lipid structures

PubMed Central

Schuster, Bernhard; Sleytr, Uwe B.

2010-01-01

Designing and utilization of biomimetic membrane systems generated by bottom-up processes is a rapidly growing scientific and engineering field. Elucidation of the supramolecular construction principle of archaeal cell envelopes composed of S-layer stabilized lipid membranes led to new strategies for generating highly stable functional lipid membranes at meso- and macroscopic scale. In this review, we provide a state of the art survey how S-layer proteins, lipids, and polysaccharides may be used as basic building blocks for the assembly of S-layer supported lipid membranes. These biomimetic membrane systems are distinguished by a nanopatterned fluidity, enhanced stability and longevity and thus, provide a dedicated reconstitution matrix for membrane-active peptides and transmembrane proteins. Exciting areas for application of composite S-layer membrane systems concern sensor systems involving specific membrane functions. PMID:19303933

On the application of a new thermal diagnostic model: the passive elements equivalent in term of ventilation inside a room

NASA Astrophysics Data System (ADS)

El Khattabi, El Mehdi; Mharzi, Mohamed; Raefat, Saad; Meghari, Zouhair

2018-05-01

In this paper, the thermal equivalence of the passive elements of a room in a building located in Fez-Morocco has been studied. The possibility of replacing them with a semi-passive element such as ventilation has been appraised. For this aim a Software in Fortran taking into account the meteorological external conditions along with different parameters of the building envelope has been performed. A new computational approach is adapted to determinate the temperature distribution throughout the building multilayer walls. A novel equation gathering the internal temperature with the external conditions, and the building envelope has been deduced in transient state.

The impact of roofing material on building energy performance

NASA Astrophysics Data System (ADS)

Badiee, Ali

The last decade has seen an increase in the efficient use of energy sources such as water, electricity, and natural gas as well as a variety of roofing materials, in the heating and cooling of both residential and commercial infrastructure. Oil costs, coal and natural gas prices remain high and unstable. All of these instabilities and increased costs have resulted in higher heating and cooling costs, and engineers are making an effort to keep them under control by using energy efficient building materials. The building envelope (that which separates the indoor and outdoor environments of a building) plays a significant role in the rate of building energy consumption. An appropriate architectural design of a building envelope can considerably lower the energy consumption during hot summers and cold winters, resulting in reduced HVAC loads. Several building components (walls, roofs, fenestration, foundations, thermal insulation, external shading devices, thermal mass, etc.) make up this essential part of a building. However, thermal insulation of a building's rooftop is the most essential part of a building envelope in that it reduces the incoming "heat flux" (defined as the amount of heat transferred per unit area per unit time from or to a surface) (Sadineni et al., 2011). Moreover, more than 60% of heat transfer occurs through the roof regardless of weather, since a roof is often the building surface that receives the largest amount of solar radiation per square annually (Suman, and Srivastava, 2009). Hence, an argument can be made that the emphasis on building energy efficiency has influenced roofing manufacturing more than any other building envelope component. This research project will address roofing energy performance as the source of nearly 60% of the building heat transfer (Suman, and Srivastava, 2009). We will also rank different roofing materials in terms of their energy performance. Other parts of the building envelope such as walls, foundation, fenestration, etc. and their thermal insulation energy performance value will not be included this study. Five different UAB campus buildings with the same reinforced concrete structure (RC Structure), each having a different roofing material were selected, surveyed, analyzed, and evaluated in this study. Two primary factors are considered in this evaluation: the energy consumption and utility bills. The data has been provided by the UAB Facilities Management Department and has been monitored from 2007 to 2013 using analysis of variance (ANOVA) and t-test methods. The energy utilities examined in this study involved electricity, domestic water, and natural gas. They were measured separately in four different seasons over a seven-year time period. The building roofing materials consisted of a green roof, a white (reflective) roof, a river rock roof, a concrete paver roof, and a traditional black roof. Results of the tested roofs from this study indicate that the white roof is the most energy efficient roofing material.

Envelope lipid-packing as a critical factor for the biological activity and stability of alphavirus particles isolated from mammalian and mosquito cells.

PubMed

Sousa, Ivanildo P; Carvalho, Carlos A M; Ferreira, Davis F; Weissmüller, Gilberto; Rocha, Gustavo M; Silva, Jerson L; Gomes, Andre M O

2011-01-21

Alphaviruses are enveloped arboviruses. The viral envelope is derived from the host cell and is positioned between two icosahedral protein shells (T = 4). Because the viral envelope contains glycoproteins involved in cell recognition and entry, the integrity of the envelope is critical for the success of the early events of infection. Differing levels of cholesterol in different hosts leads to the production of alphaviruses with distinct levels of this sterol loaded in the envelope. Using Mayaro virus, a New World alphavirus, we investigated the role of cholesterol on the envelope of alphavirus particles assembled in either mammalian or mosquito cells. Our results show that although quite different in their cholesterol content, Mayaro virus particles obtained from both cells share a similar high level of lateral organization in their envelopes. This organization, as well as viral stability and infectivity, is severely compromised when cholesterol is depleted from the envelope of virus particles isolated from mammalian cells, but virus particles isolated from mosquito cells are relatively unaffected by cholesterol depletion. We suggest that it is not cholesterol itself, but rather the organization of the viral envelope, that is critical for the biological activity of alphaviruses.

Canine distemper virus matrix protein influences particle infectivity, particle composition, and envelope distribution in polarized epithelial cells and modulates virulence.

PubMed

Dietzel, Erik; Anderson, Danielle E; Castan, Alexandre; von Messling, Veronika; Maisner, Andrea

2011-07-01

In paramyxoviruses, the matrix (M) protein mediates the interaction between the envelope and internal proteins during particle assembly and egress. In measles virus (MeV), M mutations, such as those found in subacute sclerosing panencephalitis (SSPE) strains, and differences in vaccine and wild-type M proteins can affect the strength of interaction with the envelope glycoproteins, assembly efficiency, and spread. However, the contribution of the M protein to the replication and pathogenesis of the closely related canine distemper virus (CDV) has not been characterized. To this end this, we generated a recombinant wild-type CDV carrying a vaccine strain M protein. The recombinant virus retained the parental growth phenotype in VerodogSLAMtag cells, but displayed an increased particle-to-infectivity ratio very similar to that of the vaccine strain, likely due to inefficient H protein incorporation. Even though infectious virus was released only from the apical surface, consistent with the release polarity of the wild-type CDV strain, envelope protein distribution in polarized epithelial cells reproduced the bipolar pattern seen in vaccine strain-infected cells. Most notably, the chimeric virus was completely attenuated in ferrets and caused only a mild and transient leukopenia, indicating that the differences in particle infectivity and envelope protein sorting mediated by the vaccine M protein contribute importantly to vaccine strain attenuation.

Gas recombination assembly for electrochemical cells

DOEpatents

Levy, Isaac; Charkey, Allen

1989-01-01

An assembly for recombining gases generated in electrochemical cells wherein a catalyst strip is enveloped within a hydrophobic, gas-porous film which, in turn, is encased between gas-porous, metallic layers. The sandwich construction of metallic layers and film is formed into a spiral with a tab for connection to the cell.

58. Neg. No. F160a, Jan 11, 1933, INTERIOROFFICE BUILDING/ ASSEMBLY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

58. Neg. No. F-160a, Jan 11, 1933, INTERIOR-OFFICE BUILDING/ ASSEMBLY BUILDING, FIRST AID ROOM - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Building thermography as a tool in energy audits and building commissioning procedure

NASA Astrophysics Data System (ADS)

Kauppinen, Timo

2007-04-01

A Building Commissioning-project (ToVa) was launched in Finland in the year 2003. A comprehensive commissioning procedure, including the building process and operation stage was developed in the project. This procedure will confirm the precise documentation of client's goals, definition of planning goals and the performance of the building. It is rather usual, that within 1-2 years after introduction the users complain about the defects or performance malfunctions of the building. Thermography is one important manual tool in verifying the thermal performance of the building envelope. In this paper the results of one pilot building (a school) will be presented. In surveying the condition and energy efficiency of buildings, various auxiliary means are needed. We can compare the consumption data of the target building with other, same type of buildings by benchmarking. Energy audit helps to localize and determine the energy saving potential. The most general and also most effective auxiliary means in monitoring the thermal performance of building envelopes is an infrared camera. In this presentation some examples of the use of thermography in energy audits are presented.

Rab33B Controls Hepatitis B Virus Assembly by Regulating Core Membrane Association and Nucleocapsid Processing.

PubMed

Bartusch, Christina; Döring, Tatjana; Prange, Reinhild

2017-06-21

Many viruses take advantage of cellular trafficking machineries to assemble and release new infectious particles. Using RNA interference (RNAi), we demonstrate that the Golgi/autophagosome-associated Rab33B is required for hepatitis B virus (HBV) propagation in hepatoma cell lines. While Rab33B is dispensable for the secretion of HBV subviral envelope particles, its knockdown reduced the virus yield to 20% and inhibited nucleocapsid (NC) formation and/or NC trafficking. The overexpression of a GDP-restricted Rab33B mutant phenocopied the effect of deficit Rab33B, indicating that Rab33B-specific effector proteins may be involved. Moreover, we found that HBV replication enhanced Rab33B expression. By analyzing HBV infection cycle steps, we identified a hitherto unknown membrane targeting module in the highly basic C-terminal domain of the NC-forming core protein. Rab33B inactivation reduced core membrane association, suggesting that membrane platforms participate in HBV assembly reactions. Biochemical and immunofluorescence analyses provided further hints that the viral core, rather than the envelope, is the main target for Rab33B intervention. Rab33B-deficiency reduced core protein levels without affecting viral transcription and hampered core/NC sorting to envelope-positive, intracellular compartments. Together, these results indicate that Rab33B is an important player in intracellular HBV trafficking events, guiding core transport to NC assembly sites and/or NC transport to budding sites.

BUILDING ENVELOPE OPTIMIZATION USING EMERGY ANALYSIS

EPA Science Inventory

Energy analysis is an integral component of sustainable building practices. Energy analysis coupled with optimization techniques may offer solutions for greater energy efficiency over the lifetime of the building. However, all such computationsemploy the energy used for operation...

Coupling indoor airflow, HVAC, control and building envelope heat transfer in the Modelica Buildings library

DOE PAGES

Zuo, Wangda; Wetter, Michael; Tian, Wei; ...

2015-07-13

Here, this paper describes a coupled dynamic simulation of an indoor environment with heating, ventilation, and air conditioning (HVAC) systems, controls and building envelope heat transfer. The coupled simulation can be used for the design and control of ventilation systems with stratified air distributions. Those systems are commonly used to reduce building energy consumption while improving the indoor environment quality. The indoor environment was simulated using the fast fluid dynamics (FFD) simulation programme. The building fabric heat transfer, HVAC and control system were modelled using the Modelica Buildings library. After presenting the concept, the mathematical algorithm and the implementation ofmore » the coupled simulation were introduced. The coupled FFD–Modelica simulation was then evaluated using three examples of room ventilation with complex flow distributions with and without feedback control. Lastly, further research and development needs were also discussed.« less

Coupling indoor airflow, HVAC, control and building envelope heat transfer in the Modelica Buildings library

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zuo, Wangda; Wetter, Michael; Tian, Wei

Here, this paper describes a coupled dynamic simulation of an indoor environment with heating, ventilation, and air conditioning (HVAC) systems, controls and building envelope heat transfer. The coupled simulation can be used for the design and control of ventilation systems with stratified air distributions. Those systems are commonly used to reduce building energy consumption while improving the indoor environment quality. The indoor environment was simulated using the fast fluid dynamics (FFD) simulation programme. The building fabric heat transfer, HVAC and control system were modelled using the Modelica Buildings library. After presenting the concept, the mathematical algorithm and the implementation ofmore » the coupled simulation were introduced. The coupled FFD–Modelica simulation was then evaluated using three examples of room ventilation with complex flow distributions with and without feedback control. Lastly, further research and development needs were also discussed.« less

Predictive model for CO2 generation and decay in building envelopes

NASA Astrophysics Data System (ADS)

Aglan, Heshmat A.

2003-01-01

Understanding carbon dioxide generation and decay patterns in buildings with high occupancy levels is useful to identify their indoor air quality, air change rates, percent fresh air makeup, occupancy pattern, and how a variable air volume system to off-set undesirable CO2 level can be modulated. A mathematical model governing the generation and decay of CO2 in building envelopes with forced ventilation due to high occupancy is developed. The model has been verified experimentally in a newly constructed energy efficient healthy house. It was shown that the model accurately predicts the CO2 concentration at any time during the generation and decay processes.

Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola Virus

PubMed Central

Adu-Gyamfi, Emmanuel; Johnson, Kristen A.; Fraser, Mark E.; Scott, Jordan L.; Soni, Smita P.; Jones, Keaton R.; Digman, Michelle A.; Gratton, Enrico; Tessier, Charles R.

2015-01-01

ABSTRACT Lipid-enveloped viruses replicate and bud from the host cell where they acquire their lipid coat. Ebola virus, which buds from the plasma membrane of the host cell, causes viral hemorrhagic fever and has a high fatality rate. To date, little has been known about how budding and egress of Ebola virus are mediated at the plasma membrane. We have found that the lipid phosphatidylserine (PS) regulates the assembly of Ebola virus matrix protein VP40. VP40 binds PS-containing membranes with nanomolar affinity, and binding of PS regulates VP40 localization and oligomerization on the plasma membrane inner leaflet. Further, alteration of PS levels in mammalian cells inhibits assembly and egress of VP40. Notably, interactions of VP40 with the plasma membrane induced exposure of PS on the outer leaflet of the plasma membrane at sites of egress, whereas PS is typically found only on the inner leaflet. Taking the data together, we present a model accounting for the role of plasma membrane PS in assembly of Ebola virus-like particles. IMPORTANCE The lipid-enveloped Ebola virus causes severe infection with a high mortality rate and currently lacks FDA-approved therapeutics or vaccines. Ebola virus harbors just seven genes in its genome, and there is a critical requirement for acquisition of its lipid envelope from the plasma membrane of the human cell that it infects during the replication process. There is, however, a dearth of information available on the required contents of this envelope for egress and subsequent attachment and entry. Here we demonstrate that plasma membrane phosphatidylserine is critical for Ebola virus budding from the host cell plasma membrane. This report, to our knowledge, is the first to highlight the role of lipids in human cell membranes in the Ebola virus replication cycle and draws a clear link between selective binding and transport of a lipid across the membrane of the human cell and use of that lipid for subsequent viral entry. PMID:26136573

Study of potential nonconformities of a new recreation center building's envelope

NASA Astrophysics Data System (ADS)

Stanescu, M.; Kajl, S.; Lamarche, L.

2016-09-01

This article presents a building envelope's analysis in order to verify the compliance with mandatory provisions of the Model National Energy Code for Buildings in Canada (MNECB 1997). Because some of the requirements are «not met», investigations were carried out to provide justifications in order to prove that the building can be considered as an exception to the mandatory provisions of MNECB. Therefore, we evaluate the impact of three (3) potential nonconformities of the building's walls on the building energy performance. In regards to article 3.1.1.1.4 of MNECB, there is an exception if it can be proved that permanent process (like heat recovery of refrigeration compressors) can produce at all times enough heat that no other heating source is required. First of all, by using simulation, we were able to indicate that almost all building's heating will be provided by energy recovery from ice rinks refrigeration systems (99.2%). Secondly, by using an energy analysis carried out with HEAT2 software, we can show that the increase of heating energy demand caused by the 3 studied walls is very low. This represents an increase of the heating energy demand of only 0.2%, and this, regardless of the heat recovery process. Because the nonconforming wall sections are small (0.97% of the envelope area), this mainly explains the minor impact in terms of building performance. In conclusion, according to the results obtained, we were able to recommend the building for consideration as an exception to the mandatory provisions of MNECB.

Nup53 Is Required for Nuclear Envelope and Nuclear Pore Complex Assembly

PubMed Central

Hawryluk-Gara, Lisa A.; Platani, Melpomeni; Santarella, Rachel

2008-01-01

Transport across the nuclear envelope (NE) is mediated by nuclear pore complexes (NPCs). These structures are composed of various subcomplexes of proteins that are each present in multiple copies and together establish the eightfold symmetry of the NPC. One evolutionarily conserved subcomplex of the NPC contains the nucleoporins Nup53 and Nup155. Using truncation analysis, we have defined regions of Nup53 that bind to neighboring nucleoporins as well as those domains that target Nup53 to the NPC in vivo. Using this information, we investigated the role of Nup53 in NE and NPC assembly using Xenopus egg extracts. We show that both events require Nup53. Importantly, the analysis of Nup53 fragments revealed that the assembly activity of Nup53 depleted extracts could be reconstituted using a region of Nup53 that binds specifically to its interacting partner Nup155. On the basis of these results, we propose that the formation of a Nup53–Nup155 complex plays a critical role in the processes of NPC and NE assembly. PMID:18256286

Completely assembled virus particles detected by transmission electron microscopy in proximal and mid-axons of neurons infected with herpes simplex virus type 1, herpes simplex virus type 2 and pseudorabies virus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Huang Jialing, E-mail: hjialing@mail.med.upenn.edu; Lazear, Helen M., E-mail: Hlazear@DOM.wustl.edu; Friedman, Harvey M., E-mail: hfriedma@mail.med.upenn.ed

2011-01-05

The morphology of alphaherpesviruses during anterograde axonal transport from the neuron cell body towards the axon terminus is controversial. Reports suggest that transport of herpes simplex virus type 1 (HSV-1) nucleocapsids and envelope proteins occurs in separate compartments and that complete virions form at varicosities or axon termini (subassembly transport model), while transport of a related alphaherpesvirus, pseudorabies virus (PRV) occurs as enveloped capsids in vesicles (assembled transport model). Transmission electron microscopy of proximal and mid-axons of primary superior cervical ganglion (SCG) neurons was used to compare anterograde axonal transport of HSV-1, HSV-2 and PRV. SCG cell bodies were infectedmore » with HSV-1 NS and 17, HSV-2 2.12 and PRV Becker. Fully assembled virus particles were detected intracellularly within vesicles in proximal and mid-axons adjacent to microtubules after infection with each virus, indicating that assembled virions are transported anterograde within axons for all three alphaherpesviruses.« less

Operative air temperature data for different measures applied on a building envelope in warm climate.

PubMed

Baglivo, Cristina; Congedo, Paolo Maria

2018-04-01

Several technical combinations have been evaluated in order to design high energy performance buildings for the warm climate. The analysis has been developed in several steps, avoiding the use of HVAC systems. The methodological approach of this study is based on a sequential search technique and it is shown on the paper entitled "Envelope Design Optimization by Thermal Modeling of a Building in a Warm Climate" [1]. The Operative Air Temperature trends (TOP), for each combination, have been plotted through a dynamic simulation performed using the software TRNSYS 17 (a transient system simulation program, University of Wisconsin, Solar Energy Laboratory, USA, 2010). Starting from the simplest building configuration consisting of 9 rooms (equal-sized modules of 5 × 5 m 2 ), the different building components are sequentially evaluated until the envelope design is optimized. The aim of this study is to perform a step-by-step simulation, simplifying as much as possible the model without making additional variables that can modify their performances. Walls, slab-on-ground floor, roof, shading and windows are among the simulated building components. The results are shown for each combination and evaluated for Brindisi, a city in southern Italy having 1083 degrees day, belonging to the national climatic zone C. The data show the trends of the TOP for each measure applied in the case study for a total of 17 combinations divided into eight steps.

1. MISSILE TEST AND ASSEMBLY BUILDING, FRONT, LOOKING SOUTH. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. MISSILE TEST AND ASSEMBLY BUILDING, FRONT, LOOKING SOUTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

Builders Challenge High Performance Builder Spotlight: Yavapai College, Chino Valley, Arizona

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2009-12-22

Building America Builders Challenge fact sheet on Yavapai College of Chino Valley, Arizona. These college students built a Building America Builders Challenge house that achieved the remarkably low HERS score of -3 and achieved a tight building envelope.

Endoplasmic Reticulum: The Favorite Intracellular Niche for Viral Replication and Assembly.

PubMed

Romero-Brey, Inés; Bartenschlager, Ralf

2016-06-07

The endoplasmic reticulum (ER) is the largest intracellular organelle. It forms a complex network of continuous sheets and tubules, extending from the nuclear envelope (NE) to the plasma membrane. This network is frequently perturbed by positive-strand RNA viruses utilizing the ER to create membranous replication factories (RFs), where amplification of their genomes occurs. In addition, many enveloped viruses assemble progeny virions in association with ER membranes, and viruses replicating in the nucleus need to overcome the NE barrier, requiring transient changes of the NE morphology. This review first summarizes some key aspects of ER morphology and then focuses on the exploitation of the ER by viruses for the sake of promoting the different steps of their replication cycles.

Endoplasmic Reticulum: The Favorite Intracellular Niche for Viral Replication and Assembly

PubMed Central

Romero-Brey, Inés; Bartenschlager, Ralf

2016-01-01

The endoplasmic reticulum (ER) is the largest intracellular organelle. It forms a complex network of continuous sheets and tubules, extending from the nuclear envelope (NE) to the plasma membrane. This network is frequently perturbed by positive-strand RNA viruses utilizing the ER to create membranous replication factories (RFs), where amplification of their genomes occurs. In addition, many enveloped viruses assemble progeny virions in association with ER membranes, and viruses replicating in the nucleus need to overcome the NE barrier, requiring transient changes of the NE morphology. This review first summarizes some key aspects of ER morphology and then focuses on the exploitation of the ER by viruses for the sake of promoting the different steps of their replication cycles. PMID:27338443

KSC-2010-1106

NASA Image and Video Library

2010-01-07

CAPE CANAVERAL, Fla. - In Launch Complex 39 at NASA's Kennedy Space Center in Florida, the first wall of the Propellants North Administrative and Maintenance Facility is lifted into place. In the background is the 525-foot-tall Vehicle Assembly Building. A tilt-up construction method is being used to erect a THERMOMASS concrete wall insulation system for the facility's walls. In this approach, the exterior layer of concrete for the wall panels is poured and leveled on the building's footprint. Then, prefabricated, predrilled insulation sheets are arranged on top of the unhardened concrete, and connectors, designed to hold the sandwiched layers of concrete and insulation secure, are inserted through the predrilled holes. Next, the structural wythe is poured. Once cured, these panels are lifted upright to form the building's envelope. The facility will have a two-story administrative building to house managers, mechanics and technicians who fuel spacecraft at Kennedy adjacent to an 1,800-square-foot single-story shop to store cryogenic fuel transfer equipment. The new facility will feature high-efficiency roofs and walls, “Cool Dry Quiet” air conditioning with energy recovery technology, efficient lighting, and other sustainable features. The facility is striving to qualify for the U.S. Green Building Council’s Leadership in Energy and Environmental Design, or LEED, Platinum certification. If successful, Propellants North will be the first Kennedy facility to achieve this highest of LEED ratings after it is completed in the summer of 2010. The facility was designed for NASA by Jones Edmunds and Associates. Photo credit: NASA/Jim Grossmann

A metallicity recipe for rocky planets

NASA Astrophysics Data System (ADS)

Dawson, Rebekah I.; Chiang, Eugene; Lee, Eve J.

2015-10-01

Planets with sizes between those of Earth and Neptune divide into two populations: purely rocky bodies whose atmospheres contribute negligibly to their sizes, and larger gas-enveloped planets possessing voluminous and optically thick atmospheres. We show that whether a planet forms rocky or gas-enveloped depends on the solid surface density of its parent disc. Assembly times for rocky cores are sensitive to disc solid surface density. Lower surface densities spawn smaller planetary embryos; to assemble a core of given mass, smaller embryos require more mergers between bodies farther apart and therefore exponentially longer formation times. Gas accretion simulations yield a rule of thumb that a rocky core must be at least 2M⊕ before it can acquire a volumetrically significant atmosphere from its parent nebula. In discs of low solid surface density, cores of such mass appear only after the gas disc has dissipated, and so remain purely rocky. Higher surface density discs breed massive cores more quickly, within the gas disc lifetime, and so produce gas-enveloped planets. We test model predictions against observations, using planet radius as an observational proxy for gas-to-rock content and host star metallicity as a proxy for disc solid surface density. Theory can explain the observation that metal-rich stars host predominantly gas-enveloped planets.

Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways

PubMed Central

Vlasblom, James; Gagarinova, Alla; Phanse, Sadhna; Graham, Chris; Yousif, Fouad; Ding, Huiming; Xiong, Xuejian; Nazarians-Armavil, Anaies; Alamgir, Md; Ali, Mehrab; Pogoutse, Oxana; Pe'er, Asaf; Arnold, Roland; Michaut, Magali; Parkinson, John; Golshani, Ashkan; Whitfield, Chris; Wodak, Shoshana J.; Moreno-Hagelsieb, Gabriel; Greenblatt, Jack F.; Emili, Andrew

2011-01-01

As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among >235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target. PMID:22125496

Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways.

PubMed

Babu, Mohan; Díaz-Mejía, J Javier; Vlasblom, James; Gagarinova, Alla; Phanse, Sadhna; Graham, Chris; Yousif, Fouad; Ding, Huiming; Xiong, Xuejian; Nazarians-Armavil, Anaies; Alamgir, Md; Ali, Mehrab; Pogoutse, Oxana; Pe'er, Asaf; Arnold, Roland; Michaut, Magali; Parkinson, John; Golshani, Ashkan; Whitfield, Chris; Wodak, Shoshana J; Moreno-Hagelsieb, Gabriel; Greenblatt, Jack F; Emili, Andrew

2011-11-01

As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among > 235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target.

97. JOB NO. 1347F, SHEET 5, 1927, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

97. JOB NO. 1347-F, SHEET 5, 1927, ASSEMBLY BUILDING; FORD MOTOR COMPANY; ELEVATIONS AND DOOR DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

105. JOB NO. 1347F, SHEET 15, 1927/1929, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

105. JOB NO. 1347-F, SHEET 15, 1927/1929, ASSEMBLY BUILDING; FORD MOTOR COMPANY; MISCELLANEOUS DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

98. JOB NO. 1347F, SHEET 7, 1927/1929, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

98. JOB NO. 1347-F, SHEET 7, 1927/1929, ASSEMBLY BUILDING; FORD MOTOR COMPANY; EXTERIOR DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

99. JOB NO. 1347F, SHEET 14, 1929, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

99. JOB NO. 1347-F, SHEET 14, 1929, ASSEMBLY BUILDING; FORD MOTOR COMPANY; OFFICE SECTIONS AND DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

102. JOB NO. 1347F, SHEET 3, 1927, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

102. JOB NO. 1347-F, SHEET 3, 1927, ASSEMBLY BUILDING; FORD MOTOR COMPANY; FIRST FLOOR PLAN - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

106. JOB NO. 1347F, SHEET 9, 1927, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

106. JOB NO. 1347-F, SHEET 9, 1927, ASSEMBLY BUILDING; FORD MOTOR COMPANY; TOILET-SECTION AND DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

110. JOB NO. 1347F, SHEET 11, 1927, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

110. JOB NO. 1347-F, SHEET 11, 1927, ASSEMBLY BUILDING; FORD MOTOR COMPANY; WAREHOUSE SECTION AND DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

111. JOB NO. 1347F, SHEET 12, 1927/1929, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

111. JOB NO. 1347-F, SHEET 12, 1927/1929, ASSEMBLY BUILDING; FORD MOTOR COMPANY; WAREHOUSE DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Final Technical Report. Training in Building Audit Technologies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brosemer, Kathleen

In 2011, the Tribe proposed and was awarded the Training in Building Audit Technologies grant from the DOE in the amount of $55,748 to contract for training programs for infrared cameras, blower door technology applications and building systems. The coursework consisted of; Infrared Camera Training: Level I - Thermal Imaging for Energy Audits; Blower Door Analysis and Building-As-A-System Training, Building Performance Institute (BPI) Building Analyst; Building Envelope Training, Building Performance Institute (BPI) Envelope Professional; and Audit/JobFLEX Tablet Software. Competitive procurement of the training contractor resulted in lower costs, allowing the Tribe to request and receive DOE approval to additionally purchasemore » energy audit equipment and contract for residential energy audits of 25 low-income Tribal Housing units. Sault Tribe personnel received field training to supplement the classroom instruction on proper use of the energy audit equipment. Field experience was provided through the second DOE energy audits grant, allowing Sault Tribe personnel to join the contractor, Building Science Academy, in conducting 25 residential energy audits of low-income Tribal Housing units.« less

4. MISSILE TEST AND ASSEMBLY BUILDING, LEFT SIDE, LOOKING NORTH. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. MISSILE TEST AND ASSEMBLY BUILDING, LEFT SIDE, LOOKING NORTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

2. MISSILE TEST AND ASSEMBLY BUILDING, RIGHT SIDE, LOOKING WEST. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. MISSILE TEST AND ASSEMBLY BUILDING, RIGHT SIDE, LOOKING WEST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

3. MISSILE TEST AND ASSEMBLY BUILDING, REAR SIDE, LOOKING NORTH. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. MISSILE TEST AND ASSEMBLY BUILDING, REAR SIDE, LOOKING NORTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

Numerical and experimental simulation of the mechanical behavior of super-pressure balloon subsystems

NASA Astrophysics Data System (ADS)

Siguier, J.; Guigue, P.; Karama, M.; Mistou, S.; Dalverny, O.; Granier, S.

Long duration super-pressure balloons are a great challenge in scientific ballooning. Whatever the balloon type considered (spherical, pumpkin,...), it is necessary to have good knowledge of the mechanical behavior of the envelope regarding the flight level and the life-span of the balloon. For this reason CNES, ONERA and ENIT are carrying out a research program of modelization and experimentation in order to predict the envelope shape of a balloon in different conditions of temperature and differential pressure. On the one hand, we define the mechanical laws of envelope materials, that is the elasticity, plasticity and viscosity properties of polymers, and find the parameters of the law with unidirectional tests. These laws are introduced in a finite element code which predict the stress and strain state of a complex envelope structure. On the other hand, we are developing an experimental set-up to measure the 3D strain of a balloon sub-system, that is including the envelope, assemblies and apex parts, with realistic flight conditions. This facility, called NIRVANA, is a 1m3 vacuum chamber with cooled screens equipped with a stereoscopic CCD measurement system. We can submit a 1,5m diameter sample to differential pressure, regulate the temperature from +20°C to -120°C and apply a load to tendons of up to 6 tons if required. This paper presents the first results of the modelizations and m asurements of ane envelope sample submitted to axisymetrical stress due to the differential pressure. This sample consists of a 50μm multi-layer polymer film with an assembly, used in 10m diameter STRATEOLE super-pressure balloons. The modelization gives results which largely agree with the experiment and enable us to continue with cold conditions and more complex structures.

Numerical and experimental simulation of the mechanical behavior of super-pressure balloon subsystems

NASA Astrophysics Data System (ADS)

Siguier, J.-M.; Guigue, P.; Karama, M.; Mistou, S.; Dalverny, O.; Granier, S.

2004-01-01

Long duration super-pressure balloons constitute a great challenge in scientific ballooning. For any type of balloons (spherical, pumpkin, …), it is necessary to have a good knowledge of the mechanical behavior of envelopes regarding the level and the lifetime of the flight. For this reason CNES, ONERA and ENIT are carrying out a research program of modelization and experimentation in order to predict the envelope shape of a balloon in different conditions of temperature and differential pressure. This study was conducted in two parts. During the first one, we defined, with parameters obtained from unidirectional tests, the mechanical laws (elasticity, plasticity and viscosity properties of polymers) of materials involved in the envelope. These laws are introduced in a finite element code, which predicts the stress and strain status of a complex envelope structure. During the second one, we developed an experimental set-up to measure the 3D strain on a balloon subsystem, which includes envelope, assemblies and apex parts, in real flight conditions. This facility, called NIRVANA, is a 1 m 3 vacuum chamber with cooled screens equipped with a stereoscopic CCD measurement system. A 1.5 m diameter sample can be tested under differential pressure, regulated temperature (from +20 to -120 °C) and a load (up to 6 tonnes) applied on tendons. This paper presents the first results obtained from the modelizations and measurements done on an envelope sample submitted to axisymmetrical stress due to the differential pressure. This sample consists of a 50 μm multilayer polymer film with an assembly, used in 10 m diameter STRATEOLE super-pressure balloons. The modelization gives results in good accordance with the experiments and will enable us to follow this work with cold conditions, time dependence (creeping) and more complex structures.

88. Neg. No. F124, Jul 9, 1931, INTERIORASSEMBLY BUILDING, SHOWING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

88. Neg. No. F-124, Jul 9, 1931, INTERIOR-ASSEMBLY BUILDING, SHOWING PARTIALLY ASSEMBLED CARS AND FENDER CONVEYORS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

112. JOB NO. 1347F, SHEET 16, 1929/1930, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

112. JOB NO. 1347-F, SHEET 16, 1929/1930, ASSEMBLY BUILDING; FORD MOTOR COMPANY; FREIGHT ELEVATOR DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

100. JOB NO. 1347F, SHEET 13, 1927, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

100. JOB NO. 1347-F, SHEET 13, 1927, ASSEMBLY BUILDING; FORD MOTOR COMPANY; DETAILS OF OFFICE AND SECTIONS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

108. JOB NO. 1347F, SHEET 17, 1929, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

108. JOB NO. 1347-F, SHEET 17, 1929, ASSEMBLY BUILDING; FORD MOTOR COMPANY; TUNNELs TRUSSES UNDER TANK, ETC. - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

109. JOB NO. 1347F, SHEET 8S, 1929, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

109. JOB NO. 1347-F, SHEET 8S, 1929, ASSEMBLY BUILDING; FORD MOTOR COMPANY; TRANSVERSE SECTION OF WAREHOUSE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Operating and Maintaining Energy Smart Schools Action Plan Template - All Action Plans

DOE Office of Scientific and Technical Information (OSTI.GOV)

none,

2009-07-01

EnergySmart Schools action plan templates for benchmarking, lighting, HVAC, water heating, building envelope, transformer, plug loads, kitchen equipment, swimming pool, building automation system, other.

Russia’s R&D for Low Energy Buildings: Insights for Cooperation with Russia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schaaf, Rebecca E.; Evans, Meredydd

Russian buildings, Russian buildings sector energy consumption. Russian government has made R&D investment a priority again. The government and private sector both invest in a range of building energy technologies. In particular, heating, ventilation and air conditioning, district heating, building envelope, and lighting have active technology research projects and programs in Russia.

5. MISSILE TEST AND ASSEMBLY BUILDING, FRONT AND RIGHT SIDES, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. MISSILE TEST AND ASSEMBLY BUILDING, FRONT AND RIGHT SIDES, LOOKING SOUTHEAST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

6. MISSILE TEST AND ASSEMBLY BUILDING, REAR AND LEFT SIDES, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. MISSILE TEST AND ASSEMBLY BUILDING, REAR AND LEFT SIDES, LOOKING NORTHWEST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

96. JOB NO. 1347F, SHEET 6, 1927/1929, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

96. JOB NO. 1347-F, SHEET 6, 1927/1929, ASSEMBLY BUILDING; FORD MOTOR COMPANY; NORTH, EAST, AND SOUTH ELEVATIONS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

101. JOB NO. 1347F, SHEET 3M, 1927, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

101. JOB NO. 1347-F, SHEET 3M, 1927, ASSEMBLY BUILDING; FORD MOTOR COMPANY; ONE-EIGHTH SCALE OF OFFICES - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

104. JOB NO. 1347F, SHEET 5S 1927, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

104. JOB NO. 1347-F, SHEET 5S 1927, ASSEMBLY BUILDING; FORD MOTOR COMPANY; LONGITUDINAL SECTION AND TRUSS DETAILS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

103. JOB NO. 1347F, SHEET 4, 1927/1929, ASSEMBLY BUILDING; FORD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

103. JOB NO. 1347-F, SHEET 4, 1927/1929, ASSEMBLY BUILDING; FORD MOTOR COMPANY; ROOF PLAN CROSS SECTION AND DETAIL - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cordero, Zachary C.; Meyer, Harry M.; Nandwana, Peeyush

Electrons injected into the build envelope during powder-bed electron-beam additive manufacturing can accumulate on the irradiated particles and cause them to repel each other. Furthermore, these electrostatic forces can grow so large that they drive the particles out of the build envelope in a process known as smoking. Here, a model of powder bed charging is formulated and used to develop criteria that predict the conditions under which the powder bed will smoke. These criteria suggest dependences on particle size, pre-heat temperature, and process parameters that align closely with those observed in practice.

Solution Properties of Murine Leukemia Virus Gag Protein: Differences from HIV-1 Gag▿

PubMed Central

Datta, Siddhartha A. K.; Zuo, Xiaobing; Clark, Patrick K.; Campbell, Stephen J.; Wang, Yun-Xing; Rein, Alan

2011-01-01

Immature retrovirus particles are assembled from the multidomain Gag protein. In these particles, the Gag proteins are arranged radially as elongated rods. We have previously characterized the properties of HIV-1 Gag in solution. In the absence of nucleic acid, HIV-1 Gag displays moderately weak interprotein interactions, existing in monomer-dimer equilibrium. Neutron scattering and hydrodynamic studies suggest that the protein is compact, and biochemical studies indicate that the two ends can approach close in three-dimensional space, implying the need for a significant conformational change during assembly. We now describe the properties of the Gag protein of Moloney murine leukemia virus (MLV), a gammaretrovirus. We found that this protein is very different from HIV-1 Gag: it has much weaker protein-protein interaction and is predominantly monomeric in solution. This has allowed us to study the protein by small-angle X-ray scattering and to build a low-resolution molecular envelope for the protein. We found that MLV Gag is extended in solution, with an axial ratio of ∼7, comparable to its dimensions in immature particles. Mutational analysis suggests that runs of prolines in its matrix and p12 domains and the highly charged stretch at the C terminus of its capsid domain all contribute to this extended conformation. These differences between MLV Gag and HIV-1 Gag and their implications for retroviral assembly are discussed. PMID:21917964

Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles.

PubMed

Acuña, Rodrigo; Cifuentes-Muñoz, Nicolás; Márquez, Chantal L; Bulling, Manuela; Klingström, Jonas; Mancini, Roberta; Lozach, Pierre-Yves; Tischler, Nicole D

2014-02-01

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

91. Neg. No. F174A, Apr 24, 1936, INTERIORASSEMBLY BUILDING, FINISHED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

91. Neg. No. F-174A, Apr 24, 1936, INTERIOR-ASSEMBLY BUILDING, FINISHED CARS AT THE END OF THE ASSEMBLY LINE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Proteins required for lipopolysaccharide assembly in Escherichia coli form a trans-envelope complex†

PubMed Central

Chng, Shu-Sin; Gronenberg, Luisa S.; Kahne, Daniel

2010-01-01

The viability of Gram-negative organisms is dependent on the proper placement of lipopolysaccharide (LPS) in the outer leaflet of its outer membrane. LPS is synthesized inside the cell and transported to the surface by seven essential Lpt proteins. How these proteins cooperate to transport LPS is unknown. We show that these Lpt proteins can be found in a membrane fraction that contains inner and outer membranes, and that they co-purify. This constitutes the first evidence that the Lpt proteins form a trans-envelope complex. We suggest that this protein bridge provides a route for LPS transport across the cell envelope. PMID:20446753

Structure of a bacterial virus DNA-injection protein complex reveals a decameric assembly with a constricted molecular channel

DOE PAGES

Zhao, Haiyan; Speir, Jeffrey A.; Matsui, Tsutomu; ...

2016-02-16

The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we show that a DNA-injection protein of bacteriophage Sf6, gp12, forms a 465-kDa, decameric assembly in vitro. The electron microscopic structure of the gp12 assembly shows a ~150-Å, mushroom-like architecture consisting of a crown domain and a tube-like domain, which embraces a 25-Å-wide channel that could precisely accommodate dsDNA. The constricted channel suggests that gp12 mediates rapid, uni-directional injection of phage DNA into host cells by providing a molecular conduit for DNA translocation. Themore » assembly exhibits a 10-fold symmetry, which may be a common feature among DNA-injection proteins of P22-like phages and may suggest a symmetry mismatch with respect to the 6-fold symmetric phage tail. As a result, the gp12 monomer is highly flexible in solution, supporting a mechanism for translocation of the protein through the conduit of the phage tail toward the host cell envelope, where it assembles into a DNA-injection device.« less

HSV-1 Glycoproteins Are Delivered to Virus Assembly Sites Through Dynamin-Dependent Endocytosis.

PubMed

Albecka, Anna; Laine, Romain F; Janssen, Anne F J; Kaminski, Clemens F; Crump, Colin M

2016-01-01

Herpes simplex virus-1 (HSV-1) is a large enveloped DNA virus that belongs to the family of Herpesviridae. It has been recently shown that the cytoplasmic membranes that wrap the newly assembled capsids are endocytic compartments derived from the plasma membrane. Here, we show that dynamin-dependent endocytosis plays a major role in this process. Dominant-negative dynamin and clathrin adaptor AP180 significantly decrease virus production. Moreover, inhibitors targeting dynamin and clathrin lead to a decreased transport of glycoproteins to cytoplasmic capsids, confirming that glycoproteins are delivered to assembly sites via endocytosis. We also show that certain combinations of glycoproteins colocalize with each other and with the components of clathrin-dependent and -independent endocytosis pathways. Importantly, we demonstrate that the uptake of neutralizing antibodies that bind to glycoproteins when they become exposed on the cell surface during virus particle assembly leads to the production of non-infectious HSV-1. Our results demonstrate that transport of viral glycoproteins to the plasma membrane prior to endocytosis is the major route by which these proteins are localized to the cytoplasmic virus assembly compartments. This highlights the importance of endocytosis as a major protein-sorting event during HSV-1 envelopment. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Structure of a bacterial virus DNA-injection protein complex reveals a decameric assembly with a constricted molecular channel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, Haiyan; Speir, Jeffrey A.; Matsui, Tsutomu

The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we show that a DNA-injection protein of bacteriophage Sf6, gp12, forms a 465-kDa, decameric assembly in vitro. The electron microscopic structure of the gp12 assembly shows a ~150-Å, mushroom-like architecture consisting of a crown domain and a tube-like domain, which embraces a 25-Å-wide channel that could precisely accommodate dsDNA. The constricted channel suggests that gp12 mediates rapid, uni-directional injection of phage DNA into host cells by providing a molecular conduit for DNA translocation. Themore » assembly exhibits a 10-fold symmetry, which may be a common feature among DNA-injection proteins of P22-like phages and may suggest a symmetry mismatch with respect to the 6-fold symmetric phage tail. As a result, the gp12 monomer is highly flexible in solution, supporting a mechanism for translocation of the protein through the conduit of the phage tail toward the host cell envelope, where it assembles into a DNA-injection device.« less

Demand controlled ventilating systems: Sensor market survey. Energy conservation in buildings and community systems programme, annex 18, December 1991

NASA Astrophysics Data System (ADS)

Raatschen, W.; Sjoegren, M.

The subject of indoor and outdoor air quality has generated a great deal of attention in many countries. Areas of concern include outgassing of building materials as well as occupant-generated pollutants such as carbon dioxide, moisture, and odors. Progress has also been made towards addressing issues relating to the air tightness of the building envelope. Indoor air quality studies indicate that better control of supply flow rates as well as the air distribution pattern within buildings are necessary. One method of maintaining good indoor air quality without extensive energy consumption is to control the ventilation rate according to the needs and demands of the occupants, or to preserve the building envelope. This is accomplished through the use of demand controlled ventilating (DCV) systems. The specific objective of Annex 18 is to develop guidelines for demand controlled ventilating systems based on state of the art analyses, case studies on ventilation effectiveness, and proposed ventilation rates for different users in domestic, office, and school buildings.

Indoor environmental quality (IEQ) of school classrooms: Case study in Malaysia

NASA Astrophysics Data System (ADS)

Samad, Muna Hanim Abdul; Aziz, Zalena Abdul; Isa, Mohd Hafizal Mohd

2017-10-01

Thermal Comfort is one of the key criteria for occupants' comfort and productivity in a building. In schools, it is vital for a conduciveness for teaching and learning environment. Thermal comfort is dependent on air temperature, humidity, radiation, internal lighting, air movement, activities, clothing and climatic change and is part of the Indoor Environmental Quality (IEQ) components which have has significant effects on occupants. The main concern over energy and running cost has meant that most public schools in Malaysia are designed for natural ventilation and not air-conditioning. The building envelope plays a significant role in reducing the radiant heat and keeps the interior cooler than the outdoor temperature for acceptable thermal comfort level. The requirement of Industrial Building System (IBS) as the envelope system for school building in Malaysia could affect the role of envelope as a climate moderator. This paper is based on a research conducted on two schools in Malaysia of varied construction materials as the building envelopes to ascertain the thermal comfort level of the classrooms. Elements of IEQ such as air temperature, air movement, daylighting and noise level were taken of various classrooms to fulfill the required objectives of determining the level of quality. The data collected and analysed from the study shows that in terms of air temperature which range from 28°C to 34.5°C, the schools do not achieve the recommended comfort level for tropical climate. As for daylighting element, results also show that some classrooms suffered with too much glare whilst others had insufficient daylighting. The findings also show the unsatisfactory level of air movement in the classrooms as well as an unacceptable noise level exceeding the allowable threshold. This research also concluded that the use of materials and orientation in the school design are the major determinant factors towards good IEQ levels in school buildings.

Effect of Moisture Content on Thermal Properties of Porous Building Materials

NASA Astrophysics Data System (ADS)

Kočí, Václav; Vejmelková, Eva; Čáchová, Monika; Koňáková, Dana; Keppert, Martin; Maděra, Jiří; Černý, Robert

2017-02-01

The thermal conductivity and specific heat capacity of characteristic types of porous building materials are determined in the whole range of moisture content from dry to fully water-saturated state. A transient pulse technique is used in the experiments, in order to avoid the influence of moisture transport on measured data. The investigated specimens include cement composites, ceramics, plasters, and thermal insulation boards. The effect of moisture-induced changes in thermal conductivity and specific heat capacity on the energy performance of selected building envelopes containing the studied materials is then analyzed using computational modeling of coupled heat and moisture transport. The results show an increased moisture content as a substantial negative factor affecting both thermal properties of materials and energy balance of envelopes, which underlines the necessity to use moisture-dependent thermal parameters of building materials in energy-related calculations.

Laboratory Evaluation of Energy Recovery Ventilators

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kosar, D.

Over the years, building scientists have characterized the relationship between building airtightness, exhaust-only appliances airflows, and building depressurization. Now, as the use of deep retrofit measures and new construction practices is growing to realize lower infiltration levels in increasingly tighter envelopes, performance issues can arise with the operation of exhaust-only appliances in a depressurized home. As the depressurization levels climb in tighter homes, many of these exhaust-only appliances see their rated airflows reduced and other related performance issues arise as a result. If sufficiently depressurized, atmospherically vented combustion appliances that may be present in the home can backdraft as well.more » Furthermore, when exhaust-only appliances operate and the tight home becomes depressurized, water vapor intrusion from outdoors can raise additional issues of mold in the building envelope in more humid climates.« less

Visualization of pores (export sites) correlated with cellulose production in the envelope of the gram-negative bacterium Acetobacter xylinum.

PubMed

Zaar, K

1979-03-01

The Gram-negative bacterium Acetobacter xylinum assembles a cellulse ribbon composed of a number of microfibrils in the longitudinal axis of its envelope. The zone of ribbon assembly was investigated by freeze-etch electron microscopy. Freeze-etching revealed, beneath the cellulose ribbons, a linear array of pores on the lipopolysaccharide membrane. These pores have a rim diameter of 120--150 A and a central hole or deepening of approximately 35 A. The axes of pore arrays closely coincide with linear arrays of 100 A particles on the E- and P-faces of the fractured lipopolysaccharide membranes. Pores and particles in the lipopolysaccharide membrane are probably congruent. The pores are hypothesized to be the export sites (penetration sites) for cellulose.

2. MISSILE ASSEMBLY BUILDING, RIGHT SIDE, LOOKING NORTH. NIKE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. MISSILE ASSEMBLY BUILDING, RIGHT SIDE, LOOKING NORTH. - NIKE Missile Base C-84, Missile Test & Assembly Building, North of Launch Area Entrance Drive & east of Mess Hall, Barrington, Cook County, IL

KSC-2010-1114

NASA Image and Video Library

2010-01-07

CAPE CANAVERAL, Fla. - The 525-foot-tall Vehicle Assembly Building, in the background, is witness to the formation of the Propellants North Administrative and Maintenance Facility, a new "green" building under construction in Launch Complex 39 at NASA's Kennedy Space Center in Florida. Concrete layers on either side of high-density foam insulation in the facility's walls will prevent any transfer of radiant heat between the exterior and interior of the buildings. A tilt-up construction method is being used to erect a THERMOMASS concrete wall insulation system for the facility's walls. In this approach, the exterior layer of concrete for the wall panels is poured and leveled on the building's footprint. Then, prefabricated, predrilled insulation sheets are arranged on top of the unhardened concrete, and connectors, designed to hold the sandwiched layers of concrete and insulation secure, are inserted through the predrilled holes. Next, the structural wythe is poured. Once cured, these panels are lifted upright to form the building's envelope. The facility will have a two-story administrative building to house managers, mechanics and technicians who fuel spacecraft at Kennedy adjacent to an 1,800-square-foot single-story shop to store cryogenic fuel transfer equipment. The new facility will feature high-efficiency roofs and walls, “Cool Dry Quiet” air conditioning with energy recovery technology, efficient lighting, and other sustainable features. The facility is striving to qualify for the U.S. Green Building Council’s Leadership in Energy and Environmental Design, or LEED, Platinum certification. If successful, Propellants North will be the first Kennedy facility to achieve this highest of LEED ratings after it is completed in the summer of 2010. The facility was designed for NASA by Jones Edmunds and Associates. Photo credit: NASA/Jim Grossmann

Postdoctoral Fellow | Center for Cancer Research

Cancer.gov

A postdoctoral position is currently available in a research program focused on a variety of key aspects of HIV-1 assembly and release.  Of particular interest are the interplay between viral and host factors in the targeting of assembly to the plasma membrane and the mechanism by which the viral envelope glycoproteins are incorporated into virions.  Recent studies have been

Multicriteria Analysis of Assembling Buildings from Steel Frame Structures

NASA Astrophysics Data System (ADS)

Miniotaite, Ruta

2017-10-01

Steel frame structures are often used in the construction of public and industrial buildings. They are used for: all types of slope roofs; walls of newly-built public and industrial buildings; load bearing structures; roofs of renovated buildings. The process of assembling buildings from steel frame structures should be analysed as an integrated process influenced by such factors as construction materials and machinery used, the qualification level of construction workers, complexity of work, available finance. It is necessary to find a rational technological design solution for assembling buildings from steel frame structures by conducting a multiple criteria analysis. The analysis provides a possibility to evaluate the engineering considerations and find unequivocal solutions. The rational alternative of a complex process of assembling buildings from steel frame structures was found through multiple criteria analysis and multiple criteria evaluation. In multiple criteria evaluation of technological solutions for assembling buildings from steel frame structures by pairwise comparison method the criteria by significance are distributed as follows: durability is the most important criterion in the evaluation of alternatives; the price (EUR/unit of measurement) of a part of assembly process; construction workers’ qualification level (category); mechanization level of a part of assembling process (%), and complexity of assembling work (in points) are less important criteria.

Hantavirus Gn and Gc Glycoproteins Self-Assemble into Virus-Like Particles

PubMed Central

Acuña, Rodrigo; Cifuentes-Muñoz, Nicolás; Márquez, Chantal L.; Bulling, Manuela; Klingström, Jonas; Mancini, Roberta; Lozach, Pierre-Yves

2014-01-01

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera. PMID:24335294

Solid cartridge for a pulse weld forming electrode and method of joining tubular members

DOEpatents

Bonnen, John Joseph Francis; Golovashchenko, Sergey Fedorovich; Mamutov, Alexander; Maison, Lloyd Douglas; Dawson, Scott Alwyn; deVries, James

2016-02-23

A cartridge assembly is disclosed for a pulse welding a first tube supported on a mandrel to a second tube. An outer tool is assembled over the second tube and a stored charge is discharged in the cartridge assembly. The cartridge comprises an annular conductor and a solid casing enveloping the conductor. The stored charge is electrically connected to the conductor and discharged through the conductor to compress the second tube and pulse weld the second tube to the first tube.

High Resolution Imager (HRI) for the Roentgen Satellite (ROSAT) definition study

NASA Technical Reports Server (NTRS)

1983-01-01

The design of the high resolution imager (HRI) on HEAO 2 was modified for use in the instrument complement of the Roentgen Satellite (ROSAT). Mechanical models of the front end assembly, central electronics assembly, and detector assembly were used to accurately represent the HRI envelope for both fit checks and focal plane configuration studies. The mechanical and electrical interfaces were defined and the requirements for electrical ground support equipment were established. A summary description of the ROSAT telescope and mission is included.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Badia-Martinez, Daniel; Peralta, Bibiana; Andres, German

Hepatitis C virus infects almost 170 million people per year but its assembly pathway, architecture and the structures of its envelope proteins are poorly understood. Using electron tomography of plastic-embedded sections of insect cells, we have visualized the morphogenesis of recombinant Hepatitis C virus-like particles. Our data provide a three-dimensional sketch of viral assembly at the endoplasmic reticulum showing different budding stages and contiguity of buds. This latter phenomenon could play an important role during the assembly of wt-HCV and explain the size-heterogeneity of its particles.

Hepatitis C Virus Proteins Interact with the Endosomal Sorting Complex Required for Transport (ESCRT) Machinery via Ubiquitination To Facilitate Viral Envelopment.

PubMed

Barouch-Bentov, Rina; Neveu, Gregory; Xiao, Fei; Beer, Melanie; Bekerman, Elena; Schor, Stanford; Campbell, Joseph; Boonyaratanakornkit, Jim; Lindenbach, Brett; Lu, Albert; Jacob, Yves; Einav, Shirit

2016-11-01

Enveloped viruses commonly utilize late-domain motifs, sometimes cooperatively with ubiquitin, to hijack the endosomal sorting complex required for transport (ESCRT) machinery for budding at the plasma membrane. However, the mechanisms underlying budding of viruses lacking defined late-domain motifs and budding into intracellular compartments are poorly characterized. Here, we map a network of hepatitis C virus (HCV) protein interactions with the ESCRT machinery using a mammalian-cell-based protein interaction screen and reveal nine novel interactions. We identify HRS (hepatocyte growth factor-regulated tyrosine kinase substrate), an ESCRT-0 complex component, as an important entry point for HCV into the ESCRT pathway and validate its interactions with the HCV nonstructural (NS) proteins NS2 and NS5A in HCV-infected cells. Infectivity assays indicate that HRS is an important factor for efficient HCV assembly. Specifically, by integrating capsid oligomerization assays, biophysical analysis of intracellular viral particles by continuous gradient centrifugations, proteolytic digestion protection, and RNase digestion protection assays, we show that HCV co-opts HRS to mediate a late assembly step, namely, envelopment. In the absence of defined late-domain motifs, K63-linked polyubiquitinated lysine residues in the HCV NS2 protein bind the HRS ubiquitin-interacting motif to facilitate assembly. Finally, ESCRT-III and VPS/VTA1 components are also recruited by HCV proteins to mediate assembly. These data uncover involvement of ESCRT proteins in intracellular budding of a virus lacking defined late-domain motifs and a novel mechanism by which HCV gains entry into the ESCRT network, with potential implications for other viruses. Viruses commonly bud at the plasma membrane by recruiting the host ESCRT machinery via conserved motifs termed late domains. The mechanism by which some viruses, such as HCV, bud intracellularly is, however, poorly characterized. Moreover, whether envelopment of HCV and other viruses lacking defined late domains is ESCRT mediated and, if so, what the entry points into the ESCRT pathway are remain unknown. Here, we report the interaction network of HCV with the ESCRT machinery and a critical role for HRS, an ESCRT-0 complex component, in HCV envelopment. Viral protein ubiquitination was discovered to be a signal for HRS binding and HCV assembly, thereby functionally compensating for the absence of late domains. These findings characterize how a virus lacking defined late domains co-opts ESCRT to bud intracellularly. Since the ESCRT machinery is essential for the life cycle of multiple viruses, better understanding of this virus-host interplay may yield targets for broad-spectrum antiviral therapies. Copyright © 2016 Barouch-Bentov et al.

Virtual auditorium concepts for exhibition halls

NASA Astrophysics Data System (ADS)

Evans, Jack; Himmel, Chad; Knight, Sarah

2002-11-01

Many communities lack good performance facilities for symphonic music, opera, dramatic and musical arts, but have basic convention, exhibition or assembly spaces. It should be possible to develop performance space environments within large multipurpose facilities that will accommodate production and presentation of dramatic arts. Concepts for moderate-cost, temporary enhancements that transform boxy spaces into more intimate, acoustically articulated venues will be presented. Acoustical criteria and design parameters will be discussed in the context of creating a virtual auditorium within the building envelope. Physical, economic, and logistical limitations affect implementation. Sound reinforcement system augmentation can supplement the room conversion. Acceptable control of reflection patterns, reverberation, and to some extent, ambient noise, may be achieved with an array of nonpermanent reflector and absorber elements. These elements can sculpture an enclosure to approach the shape and acoustic characteristics of an auditorium. Plan and section illustrations will be included.

81. Neg. No. F63, Apr 13, 1930, INTERIORASSEMBLY BUILDING, HOOD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

81. Neg. No. F-63, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, HOOD DEPARTMENT - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Assessment of energy and economic performance of office building models: a case study

NASA Astrophysics Data System (ADS)

Song, X. Y.; Ye, C. T.; Li, H. S.; Wang, X. L.; Ma, W. B.

2016-08-01

Energy consumption of building accounts for more than 37.3% of total energy consumption while the proportion of energy-saving buildings is just 5% in China. In this paper, in order to save potential energy, an office building in Southern China was selected as a test example for energy consumption characteristics. The base building model was developed by TRNSYS software and validated against the recorded data from the field work in six days out of August-September in 2013. Sensitivity analysis was conducted for energy performance of building envelope retrofitting; five envelope parameters were analyzed for assessing the thermal responses. Results indicated that the key sensitivity factors were obtained for the heat-transfer coefficient of exterior walls (U-wall), infiltration rate and shading coefficient (SC), of which the sum sensitivity factor was about 89.32%. In addition, the results were evaluated in terms of energy and economic analysis. The analysis of sensitivity validated against some important results of previous studies. On the other hand, the cost-effective method improved the efficiency of investment management in building energy.

Mutations altering the gammaretrovirus endoproteolytic motif affect glycosylation of the envelope glycoprotein and early events of the virus life cycle

DOE Office of Scientific and Technical Information (OSTI.GOV)

Argaw, Takele; Wilson, Carolyn A., E-mail: carolyn.wilson@fda.hhs.gov

Previously, we found that mutation of glutamine to proline in the endoproteolytic cleavage signal of the PERV-C envelope (RQKK to RPKK) resulted in non-infectious vectors. Here, we show that RPKK results in a non-infectious vector when placed in not only a PERV envelope, but also the envelope of a related gammaretrovirus, FeLV-B. The amino acid substitutions do not prevent envelope precursor cleavage, viral core and genome assembly, or receptor binding. Rather, the mutations result in the formation of hyperglycosylated glycoprotein and a reduction in the reverse transcribed minus strand synthesis and undetectable 2-LTR circular DNA in cells exposed to vectorsmore » with these mutated envelopes. Our findings suggest novel functions associated with the cleavage signal sequence that may affect trafficking through the glycosylation machinery of the cell. Further, the glycosylation status of the envelope appears to impact post-binding events of the viral life cycle, either membrane fusion, internalization, or reverse transcription. - Highlights: • Env cleavage signal impacts infectivity of gammaretroviruses. • Non-infectious mutants have hyper-glycosylated envelope that bind target cells. • Non-infectious mutants have defects in the formation of the double-stranded DNA. • Env cleavage motif has functions beyond cleavage of the env precursor.« less

Energy Conscious Design: Educational Facilities. [Brief No.] 1.

ERIC Educational Resources Information Center

American Inst. of Architects, Washington, DC.

An energy task group of the American Institute of Architects discusses design features and options that educational facility designers can use to create an energy efficient school building. Design elements cover the building envelope, energy storage system, hydronic heating/cooling systems, solar energy collection, building orientation and shape,…

83. Neg. No. F53, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BACK ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

83. Neg. No. F-53, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BACK TRIM LINE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

85. Neg. No. F51, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

85. Neg. No. F-51, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BODY AND CUSHION LINE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

79. Neg. No. F61A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

79. Neg. No. F-61A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BODY CONSTRUCTION - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

89. Neg. No. F126, Jul 21, 1931, INTERIORASSEMBLY BUILDING, SHOWING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

89. Neg. No. F-126, Jul 21, 1931, INTERIOR-ASSEMBLY BUILDING, SHOWING HEADLIGHT TESTING BOOTH - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

92. Neg. No. F142, Mar 29, 1932, INTERIORASSEMBLY BUILDING, STAGE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

92. Neg. No. F-142, Mar 29, 1932, INTERIOR-ASSEMBLY BUILDING, STAGE AND EXHIBITION ROOM - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

86. Neg. No. F64, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

86. Neg. No. F-64, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BODY STORAGE CONVEYOR - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Hepatitis C Virus Proteins Interact with the Endosomal Sorting Complex Required for Transport (ESCRT) Machinery via Ubiquitination To Facilitate Viral Envelopment

PubMed Central

Barouch-Bentov, Rina; Neveu, Gregory; Xiao, Fei; Beer, Melanie; Bekerman, Elena; Schor, Stanford; Campbell, Joseph; Boonyaratanakornkit, Jim; Lindenbach, Brett; Lu, Albert; Jacob, Yves

2016-01-01

ABSTRACT Enveloped viruses commonly utilize late-domain motifs, sometimes cooperatively with ubiquitin, to hijack the endosomal sorting complex required for transport (ESCRT) machinery for budding at the plasma membrane. However, the mechanisms underlying budding of viruses lacking defined late-domain motifs and budding into intracellular compartments are poorly characterized. Here, we map a network of hepatitis C virus (HCV) protein interactions with the ESCRT machinery using a mammalian-cell-based protein interaction screen and reveal nine novel interactions. We identify HRS (hepatocyte growth factor-regulated tyrosine kinase substrate), an ESCRT-0 complex component, as an important entry point for HCV into the ESCRT pathway and validate its interactions with the HCV nonstructural (NS) proteins NS2 and NS5A in HCV-infected cells. Infectivity assays indicate that HRS is an important factor for efficient HCV assembly. Specifically, by integrating capsid oligomerization assays, biophysical analysis of intracellular viral particles by continuous gradient centrifugations, proteolytic digestion protection, and RNase digestion protection assays, we show that HCV co-opts HRS to mediate a late assembly step, namely, envelopment. In the absence of defined late-domain motifs, K63-linked polyubiquitinated lysine residues in the HCV NS2 protein bind the HRS ubiquitin-interacting motif to facilitate assembly. Finally, ESCRT-III and VPS/VTA1 components are also recruited by HCV proteins to mediate assembly. These data uncover involvement of ESCRT proteins in intracellular budding of a virus lacking defined late-domain motifs and a novel mechanism by which HCV gains entry into the ESCRT network, with potential implications for other viruses. PMID:27803188

Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola Virus.

PubMed

Adu-Gyamfi, Emmanuel; Johnson, Kristen A; Fraser, Mark E; Scott, Jordan L; Soni, Smita P; Jones, Keaton R; Digman, Michelle A; Gratton, Enrico; Tessier, Charles R; Stahelin, Robert V

2015-09-01

Lipid-enveloped viruses replicate and bud from the host cell where they acquire their lipid coat. Ebola virus, which buds from the plasma membrane of the host cell, causes viral hemorrhagic fever and has a high fatality rate. To date, little has been known about how budding and egress of Ebola virus are mediated at the plasma membrane. We have found that the lipid phosphatidylserine (PS) regulates the assembly of Ebola virus matrix protein VP40. VP40 binds PS-containing membranes with nanomolar affinity, and binding of PS regulates VP40 localization and oligomerization on the plasma membrane inner leaflet. Further, alteration of PS levels in mammalian cells inhibits assembly and egress of VP40. Notably, interactions of VP40 with the plasma membrane induced exposure of PS on the outer leaflet of the plasma membrane at sites of egress, whereas PS is typically found only on the inner leaflet. Taking the data together, we present a model accounting for the role of plasma membrane PS in assembly of Ebola virus-like particles. The lipid-enveloped Ebola virus causes severe infection with a high mortality rate and currently lacks FDA-approved therapeutics or vaccines. Ebola virus harbors just seven genes in its genome, and there is a critical requirement for acquisition of its lipid envelope from the plasma membrane of the human cell that it infects during the replication process. There is, however, a dearth of information available on the required contents of this envelope for egress and subsequent attachment and entry. Here we demonstrate that plasma membrane phosphatidylserine is critical for Ebola virus budding from the host cell plasma membrane. This report, to our knowledge, is the first to highlight the role of lipids in human cell membranes in the Ebola virus replication cycle and draws a clear link between selective binding and transport of a lipid across the membrane of the human cell and use of that lipid for subsequent viral entry. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Powder bed charging during electron-beam additive manufacturing

DOE PAGES

Cordero, Zachary C.; Meyer, Harry M.; Nandwana, Peeyush; ...

2016-11-18

Electrons injected into the build envelope during powder-bed electron-beam additive manufacturing can accumulate on the irradiated particles and cause them to repel each other. Furthermore, these electrostatic forces can grow so large that they drive the particles out of the build envelope in a process known as smoking. Here, a model of powder bed charging is formulated and used to develop criteria that predict the conditions under which the powder bed will smoke. These criteria suggest dependences on particle size, pre-heat temperature, and process parameters that align closely with those observed in practice.

Passivhaus: indoor comfort and energy dynamic analysis.

NASA Astrophysics Data System (ADS)

Guida, Antonella; Pagliuca, Antonello; Cardinale, Nicola; Rospi, Gianluca

2013-04-01

The research aims to verify the energy performance as well as the indoor comfort of an energy class A+ building, built so that the sum of the heat passive contributions of solar radiation, transmitted through the windows, and the heat generated inside the building, are adeguate to compensate for the envelope loss during the cold season. The building, located in Emilia Romagna (Italy), was built using a wooden structure, an envelope realized using a pinewood sandwich panels (transmittance U = 0.250 W/m2K) and, inside, a wool flax insulation layer and thermal window frame with low-emissivity glass (U = 0524 W/m2K). The building design and construction process has followed the guidelines set by "CasaClima". The building has been modeled in the code of dynamic calculation "Energy Plus" by the Design Builder application and divided it into homogenous thermal zones, characterized by winter indoor temperature set at 20 ° (+ / - 1 °) and summer indoor temperature set at 26 ° (+ / - 1 °). It has modeled: the envelope, as described above, the "free" heat contributions, the air conditioning system, the Mechanical Ventilation system as well as home automation solutions. The air conditioning system is an heat pump, able to guarantee an optimization of energy consumption (in fact, it uses the "free" heat offered by the external environment for conditioning indoor environment). As regards the air recirculation system, it has been used a mechanical ventilation system with internal heat cross-flow exchanger, with an efficiency equal to 50%. The domotic solutions, instead, regard a system for the control of windows external screening using reeds, adjustable as a function of incident solar radiation and a lighting management system adjusted automatically using a dimmer. A so realized building meets the requirement imposed from Italian standard UNI/TS 11300 1, UNI/TS 11300 2 and UNI/TS 11300 3. The analysis was performed according to two different configurations: in "spontaneous-state analysis" (that provides the only energy performance of the structure) and considering the "building-equipments" as a system (which provides the overall performance of the "building system"). The first analysis shows as the absence of thermal mass and the envelope super-heating prevent to incoming heat to exit, overheating the indoor environment. The analysis of the overall performance of the "building system" highlights, instead, as the thermal load is much greater during the summer than in winter; this means that, using a low inertia envelopes, the energy saved in the winter can be used to satisfy the thermal performance in the summer. This is further demonstrated by comparing the performance of indoor temperatures and the relative energy consumption of a similar building with greater thermal inertia. Further analysis involved a critical comparison between the "semisteady-state analysis" ("CasaClima" methodology) and the analysis in dynamic conditions (using "Energy Plus" software).

65. Neg. No. F99, Dec 27, 1931, INTERIORPRESSED STEEL BUILDING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

65. Neg. No. F-99, Dec 27, 1931, INTERIOR-PRESSED STEEL BUILDING FACING SOUTH TOWARD WAREHOUSE AND ASSEMBLY BUILDING 1ST FLOOR, EAST SIDE FACING SOUTH, SHOWING PRESSED STEEL MACHINERY AND RAILROAD CARS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

94. Neg. No. F130, Sep 24, 1931, EXTERIOROFFICE BUILDING AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

94. Neg. No. F-130, Sep 24, 1931, EXTERIOR-OFFICE BUILDING AND ASSEMBLY BUILDING, WEST SIDE, SHOWING TRUCKS AND TRAILORS LOADED WITH NEW TRUCKS DISPLAYING SIGNS 'MORE FORDS FOR HOOVER DAM' - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

76. Neg. No. F58, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BURNOFF, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

76. Neg. No. F-58, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BURNOFF, LOAD END OF ENAMEL OVEN - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

77. Neg. No. F65A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, UNLOADING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

77. Neg. No. F-65A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, UNLOADING THE ENAMEL OVEN - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

90. Neg. No. F59A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, CHASSIS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

90. Neg. No. F-59A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, CHASSIS LINE, LOOKING SOUTH - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

87. Neg. No. F74A, Jun 14, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

87. Neg. No. F-74A, Jun 14, 1930, INTERIOR-ASSEMBLY BUILDING, BODY STORAGE CONVEYORS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

84. Neg. No. F62, Apr 13, 1930, INTERIORASSEMBLY BUILDING, FRAME ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

84. Neg. No. F-62, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, FRAME AND MOTOR STORAGE CONVEYOR - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dyson, Anna

Intelligent Facades for High Performance Green Buildings: Previous research and development of intelligent facades systems has been limited in their contribution towards national goals for achieving on-site net zero buildings, because this R&D has failed to couple the many qualitative requirements of building envelopes such as the provision of daylighting, access to exterior views, satisfying aesthetic and cultural characteristics, with the quantitative metrics of energy harvesting, storage and redistribution. To achieve energy self-sufficiency from on-site solar resources, building envelopes can and must address this gamut of concerns simultaneously. With this project, we have undertaken a high-performance building- integrated combined-heat andmore » power concentrating photovoltaic system with high temperature thermal capture, storage and transport towards multiple applications (BICPV/T). The critical contribution we are offering with the Integrated Concentrating Solar Façade (ICSF) is conceived to improve daylighting quality for improved health of occupants and mitigate solar heat gain while maximally capturing and transferring on- site solar energy. The ICSF accomplishes this multi-functionality by intercepting only the direct-normal component of solar energy (which is responsible for elevated cooling loads) thereby transforming a previously problematic source of energy into a high- quality resource that can be applied to building demands such as heating, cooling, dehumidification, domestic hot water, and possible further augmentation of electrical generation through organic Rankine cycles. With the ICSF technology, our team is addressing the global challenge in transitioning commercial and residential building stock towards on-site clean energy self-sufficiency, by fully integrating innovative environmental control systems strategies within an intelligent and responsively dynamic building envelope. The advantage of being able to use the entire solar spectrum for active and passive benefits, along with the potential savings of avoiding transmission losses through direct current (DC) transfer to all buildings systems directly from the site of solar conversion, gives the system a compounded economic viability within the commercial and institutional building markets.« less

Analysis of building envelope insulation performance utilizing integrated temperature and humidity sensors.

PubMed

Hung, San-Shan; Chang, Chih-Yuan; Hsu, Cheng-Jui; Chen, Shih-Wei

2012-01-01

A major cause of high energy consumption for air conditioning in indoor spaces is the thermal storage characteristics of a building's envelope concrete material; therefore, the physiological signals (temperature and humidity) within concrete structures are an important reference for building energy management. The current approach to measuring temperature and humidity within concrete structures (i.e., thermocouples and fiber optics) is limited by problems of wiring requirements, discontinuous monitoring, and high costs. This study uses radio frequency integrated circuits (RFIC) combined with temperature and humidity sensors (T/H sensors) for the design of a smart temperature and humidity information material (STHIM) that automatically, regularly, and continuously converts temperature and humidity signals within concrete and transmits them by radio frequency (RF) to the Building Physiology Information System (BPIS). This provides a new approach to measurement that incorporates direct measurement, wireless communication, and real-time continuous monitoring to assist building designers and users in making energy management decisions and judgments.

Analysis of Building Envelope Insulation Performance Utilizing Integrated Temperature and Humidity Sensors

PubMed Central

Hung, San-Shan; Chang, Chih-Yuan; Hsu, Cheng-Jui; Chen, Shih-Wei

2012-01-01

A major cause of high energy consumption for air conditioning in indoor spaces is the thermal storage characteristics of a building's envelope concrete material; therefore, the physiological signals (temperature and humidity) within concrete structures are an important reference for building energy management. The current approach to measuring temperature and humidity within concrete structures (i.e., thermocouples and fiber optics) is limited by problems of wiring requirements, discontinuous monitoring, and high costs. This study uses radio frequency integrated circuits (RFIC) combined with temperature and humidity sensors (T/H sensors) for the design of a smart temperature and humidity information material (STHIM) that automatically, regularly, and continuously converts temperature and humidity signals within concrete and transmits them by radio frequency (RF) to the Building Physiology Information System (BPIS). This provides a new approach to measurement that incorporates direct measurement, wireless communication, and real-time continuous monitoring to assist building designers and users in making energy management decisions and judgments. PMID:23012529

82. Neg. No. F66A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, TRIM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

82. Neg. No. F-66A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, TRIM LINE AND GLASS DEPARTMENTS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

78. Neg. No. F143A, Apr 1, 1932, INTERIORASSEMBLY BUILDING, CONVEYOR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

78. Neg. No. F-143A, Apr 1, 1932, INTERIOR-ASSEMBLY BUILDING, CONVEYOR LINE WITH CAR CHASSIS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Energy efficiency façade design in high-rise apartment buildings using the calculation of solar heat transfer through windows with shading devices

NASA Astrophysics Data System (ADS)

Ha, P. T. H.

2018-04-01

The architectural design orientation at the first design stage plays a key role and has a great impact on the energy consumption of a building throughout its life-cycle. To provide designers with a simple and useful tool in quantitatively determining and simply optimizing the energy efficiency of a building at the very first stage of conceptual design, a factor namely building envelope energy efficiency (Khqnl ) should be investigated and proposed. Heat transfer through windows and other glazed areas of mezzanine floors accounts for 86% of overall thermal transfer through building envelope, so the factor Khqnl of high-rise buildings largely depends on shading solutions. The author has established tables and charts to make reference to the values of Khqnl factor in certain high-rise apartment buildings in Hanoi calculated with a software program subject to various inputs including: types and sizes of shading devices, building orientations and at different points of time to be respectively analyzed. It is possible and easier for architects to refer to these tables and charts in façade design for a higher level of energy efficiency.

Synergic effects of thermal mass and natural ventilation on the thermal behaviour of traditional massive buildings

NASA Astrophysics Data System (ADS)

Gagliano, A.; Nocera, F.; Patania, F.; Moschella, A.; Detommaso, M.; Evola, G.

2016-05-01

The energy policies about energy efficiency in buildings currently focus on new buildings and on existing buildings in case of energy retrofit. However, historic and heritage buildings, that are the trademark of numerous European cities, should also deserve attention; nevertheless, their energy efficiency is nowadays not deeply investigated. In this context, this study evaluates the thermal performance of a traditional massive building situated in a Mediterranean city. Dynamic numerical simulations were carried out on a yearly basis through the software DesignBuilder, both in free-running conditions and in the presence of an air-conditioning (AC) system. The results highlight that the massive envelope of traditional residential buildings helps in maintaining small fluctuations of the indoor temperature, thus limiting the need for AC in the mid-season and in summer. This feature is highly emphasised by exploiting natural ventilation at night, which allows reducing the building energy demand for cooling by about 30%.The research also indicates that, for Mediterranean climate, the increase in thermal insulation does not always induce positive effects on the thermal performance in summer, and that it might even produce an increase in the heat loads due to the transmission through the envelope.

Photovoltaic building sheathing element with anti-slide features

DOEpatents

Keenihan, James R.; Langmaid, Joseph A.; Lopez, Leonardo C.

2015-09-08

The present invention is premised` upon an assembly that includes at least a photovoltaic building sheathing element capable of being affixed on a building structure, the photovoltaic building sheathing element. The element including a photovoltaic cell assembly, a body portion attached to one or more portions of the photovoltaic cell assembly; and at feast a first and a second connector assembly capable of directly or indirectly electrically connecting the photovoltaic cell assembly to one or more adjoining devices; wherein the body portion includes one or more geometric features adapted to engage a vertically adjoining device before installation.

Single molecule fate of HIV-1 envelope reveals late-stage viral lattice incorporation.

PubMed

Buttler, Carmen A; Pezeshkian, Nairi; Fernandez, Melissa V; Aaron, Jesse; Norman, Sofya; Freed, Eric O; van Engelenburg, Schuyler B

2018-05-10

Human immunodeficiency virus type 1 (HIV-1) assembly occurs on the inner leaflet of the host cell plasma membrane, incorporating the essential viral envelope glycoprotein (Env) within a budding lattice of HIV-1 Gag structural proteins. The mechanism by which Env incorporates into viral particles remains poorly understood. To determine the mechanism of recruitment of Env to assembly sites, we interrogate the subviral angular distribution of Env on cell-associated virus using multicolor, three-dimensional (3D) superresolution microscopy. We demonstrate that, in a manner dependent on cell type and on the long cytoplasmic tail of Env, the distribution of Env is biased toward the necks of cell-associated particles. We postulate that this neck-biased distribution is regulated by vesicular retention and steric complementarity of Env during independent Gag lattice formation.

Postdoctoral Fellow | Center for Cancer Research

Cancer.gov

A postdoctoral position is currently available in a research program focused on a variety of key aspects of HIV-1 assembly and release.  Of particular interest are the interplay between viral and host factors in the targeting of assembly to the plasma membrane and the mechanism by which the viral envelope glycoproteins are incorporated into virions.  Recent studies have been aimed at defining the cellular pathways and host factors involved in envelope glycoprotein incorporation and the budding of retrovirus particles from the plasma membrane and identifying inhibitors of virus budding and entry.  Mechanisms of HIV-1 drug resistance are also under investigation, and studies are underway to define the target and mechanism of action of a novel HIV-1 maturation inhibitor.  Further details and a list of relevant publications can be found at http://home.ncifcrf.gov/hivdrp/Freed.html.

L2, the minor capsid protein of papillomavirus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Joshua W.; Roden, Richard B.S., E-mail: roden@jhmi.edu; Department of Oncology, The Johns Hopkins University, Baltimore, MD 21287

2013-10-15

The capsid protein L2 plays major roles in both papillomavirus assembly and the infectious process. While L1 forms the majority of the capsid and can self-assemble into empty virus-like particles (VLPs), L2 is a minor capsid component and lacks the capacity to form VLPs. However, L2 co-assembles with L1 into VLPs, enhancing their assembly. L2 also facilitates encapsidation of the ∼8 kbp circular and nucleosome-bound viral genome during assembly of the non-enveloped T=7d virions in the nucleus of terminally differentiated epithelial cells, although, like L1, L2 is not detectably expressed in infected basal cells. With respect to infection, L2 ismore » not required for particles to bind to and enter cells. However L2 must be cleaved by furin for endosome escape. L2 then travels with the viral genome to the nucleus, wherein it accumulates at ND-10 domains. Here, we provide an overview of the biology of L2. - Highlights: • L2 is the minor antigen of the non-enveloped T=7d icosahedral Papillomavirus capsid. • L2 is a nuclear protein that can traffic to ND-10 and facilitate genome encapsidation. • L2 is critical for infection and must be cleaved by furin. • L2 is a broadly protective vaccine antigen recognized by neutralizing antibodies.« less

A Point Mutation in the N-Terminal Amphipathic Helix α0 in NS3 Promotes Hepatitis C Virus Assembly by Altering Core Localization to the Endoplasmic Reticulum and Facilitating Virus Budding

PubMed Central

Yan, Yu; He, Ying; Boson, Bertrand; Wang, Xuesong; Cosset, François-Loïc

2017-01-01

ABSTRACT The assembly of hepatitis C virus (HCV), a complicated process in which many viral and cellular factors are involved, has not been thoroughly deciphered. NS3 is a multifunctional protein that contains an N-terminal amphipathic α helix (designated helix α0), which is crucial for the membrane association and stability of NS3 protein, followed by a serine protease domain and a C-terminal helicase/NTPase domain. NS3 participates in HCV assembly likely through its C-terminal helicase domain, in which all reported adaptive mutations enhancing virion assembly reside. In this study, we determined that the N-terminal helix α0 of NS3 may contribute to HCV assembly. We identified a single mutation from methionine to threonine at amino acid position 21 (M21T) in helix α0, which significantly promoted viral production while having no apparent effect on the membrane association and protease activity of NS3. Subsequent analyses demonstrated that the M21T mutation did not affect HCV genome replication but rather promoted virion assembly. Further study revealed a shift in the subcellular localization of core protein from lipid droplets (LD) to the endoplasmic reticulum (ER). Finally, we showed that the M21T mutation increased the colocalization of core proteins and viral envelope proteins, leading to a more efficient envelopment of viral nucleocapsids. Collectively, the results of our study revealed a new function of NS3 helix α0 and aid understanding of the role of NS3 in HCV virion morphogenesis. IMPORTANCE HCV NS3 protein possesses the protease activity in its N-terminal domain and the helicase activity in its C-terminal domain. The role of NS3 in virus assembly has been mainly attributed to its helicase domain, because all adaptive mutations enhancing progeny virus production are found to be within this domain. Our study identified, for the first time to our knowledge, an adaptive mutation within the N-terminal helix α0 domain of NS3 that significantly enhanced virus assembly while having no effect on viral genome replication. The mechanistic studies suggested that this mutation promoted the relocation of core proteins from LD to the ER, leading to a more efficient envelopment of viral nucleocapsids. Our results revealed a possible new function of helix α0 in the HCV life cycle and provided new clues to understanding the molecular mechanisms for the action of NS3 in HCV assembly. PMID:28053108

The Problem and Needs of Existing Urban Multifamily Building Estates in Poland

NASA Astrophysics Data System (ADS)

Rybka, Adam; Rybka, Sławomir

2012-06-01

The present paper explains in a descriptive way the findings and derivations, concerning the various needs, solutions and priorities which are identified in existing urban building envelopes of Poland, especially in Rzeszow housing estates.

BORED AND ASSEMBLED GATE VALVES RECEIVING PROTECTIVE COATING IN THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

BORED AND ASSEMBLED GATE VALVES RECEIVING PROTECTIVE COATING IN THE VALVE PAINT BOOTH OF THE VALVE ASSEMBLY BUILDING. - Stockham Pipe & Fittings Company, Valve Assembly Building, 4000 Tenth Avenue North, Birmingham, Jefferson County, AL

Effect of heat and moisture transport and storage properties of building stones on the hygrothermal performance of historical building envelopes

NASA Astrophysics Data System (ADS)

KoÅáková, Dana; Kočí, Václav; Žumár, Jaromír; Keppert, Martin; Holčapek, Ondřej; Vejmelková, Eva; Černý, Robert

2016-12-01

The heat and moisture transport and storage parameters of three different natural stones used on the Czech territory since medieval times are determined experimentally, together with the basic physical properties and mechanical parameters. The measured data are applied as input parameters in the computational modeling of hygrothermal performance of building envelopes made of the analyzed stones. Test reference year climatic data of three different locations within the Czech Republic are used as boundary conditions on the exterior side. Using the simulated hygric and thermal performance of particular stone walls, their applicability is assessed in a relation to the geographical and climatic conditions. The obtained results indicate that all three investigated stones are highly resistant to weather conditions, freeze/thaw cycles in particular.

Analysis of Numerical Models for Dispersion of Chemical/Biological Agents in Complex Building Environments

DTIC Science & Technology

2004-11-01

variation in ventilation rates over time and the distribution of ventilation air within a building, and to estimate the impact of envelope air ... tightening efforts on infiltration rates. • It may be used to determine the indoor air quality performance of a building before construction, and to

10 CFR 434.516 - Building exterior envelope.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Prototype and Reference Buildings, the infiltration assumptions in subsection 516.2.1 shall be prescribed.... Infiltration shall impact perimeter zones only. 516.2.1When the HVAC system is switched “on,” no infiltration shall be assumed. When the HVAC system is switched “off,” the infiltration rate for buildings with or...

10 CFR 434.516 - Building exterior envelope.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Prototype and Reference Buildings, the infiltration assumptions in subsection 516.2.1 shall be prescribed.... Infiltration shall impact perimeter zones only. 516.2.1When the HVAC system is switched “on,” no infiltration shall be assumed. When the HVAC system is switched “off,” the infiltration rate for buildings with or...

10 CFR 434.516 - Building exterior envelope.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Prototype and Reference Buildings, the infiltration assumptions in subsection 516.2.1 shall be prescribed.... Infiltration shall impact perimeter zones only. 516.2.1When the HVAC system is switched “on,” no infiltration shall be assumed. When the HVAC system is switched “off,” the infiltration rate for buildings with or...

10 CFR 434.516 - Building exterior envelope.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Prototype and Reference Buildings, the infiltration assumptions in subsection 516.2.1 shall be prescribed.... Infiltration shall impact perimeter zones only. 516.2.1When the HVAC system is switched “on,” no infiltration shall be assumed. When the HVAC system is switched “off,” the infiltration rate for buildings with or...

Innovative Session 7. [Concurrent Innovative Session at AHRD Annual Conference, 2000.

ERIC Educational Resources Information Center

2000

This document consists of two papers on theory building from a conference on human resource development (HRD). Both "Theory Building Research in HRD--Pushing the Envelope!" (Richard A. Swanson, Susan A. Lynham, Wendy E. A. Ruona, Richard J. Torraco) and "The Role of Theory Building in Maturing the Human Resource Development…

Robotically Assembled Aerospace Structures: Digital Material Assembly using a Gantry-Type Assembler

NASA Technical Reports Server (NTRS)

Trinh, Greenfield; Copplestone, Grace; O'Connor, Molly; Hu, Steven; Nowak, Sebastian; Cheung, Kenneth; Jenett, Benjamin; Cellucci, Daniel

2017-01-01

This paper evaluates the development of automated assembly techniques for discrete lattice structures using a multi-axis gantry type CNC machine. These lattices are made of discrete components called digital materials. We present the development of a specialized end effector that works in conjunction with the CNC machine to assemble these lattices. With this configuration we are able to place voxels at a rate of 1.5 per minute. The scalability of digital material structures due to the incremental modular assembly is one of its key traits and an important metric of interest. We investigate the build times of a 5x5 beam structure on the scale of 1 meter (325 parts), 10 meters (3,250 parts), and 30 meters (9,750 parts). Utilizing the current configuration with a single end effector, performing serial assembly with a globally fixed feed station at the edge of the build volume, the build time increases according to a scaling law of n4, where n is the build scale. Build times can be reduced significantly by integrating feed systems into the gantry itself, resulting in a scaling law of n3. A completely serial assembly process will encounter time limitations as build scale increases. Automated assembly for digital materials can assemble high performance structures from discrete parts, and techniques such as built in feed systems, parallelization, and optimization of the fastening process will yield much higher throughput.

Robotically Assembled Aerospace Structures: Digital Material Assembly using a Gantry-Type Assembler

NASA Technical Reports Server (NTRS)

Trinh, Greenfield; Copplestone, Grace; O'Connor, Molly; Hu, Steven; Nowak, Sebastian; Cheung, Kenneth; Jenett, Benjamin; Cellucci, Daniel

2017-01-01

This paper evaluates the development of automated assembly techniques for discrete lattice structures using a multi-axis gantry type CNC machine. These lattices are made of discrete components called "digital materials." We present the development of a specialized end effector that works in conjunction with the CNC machine to assemble these lattices. With this configuration we are able to place voxels at a rate of 1.5 per minute. The scalability of digital material structures due to the incremental modular assembly is one of its key traits and an important metric of interest. We investigate the build times of a 5x5 beam structure on the scale of 1 meter (325 parts), 10 meters (3,250 parts), and 30 meters (9,750 parts). Utilizing the current configuration with a single end effector, performing serial assembly with a globally fixed feed station at the edge of the build volume, the build time increases according to a scaling law of n4, where n is the build scale. Build times can be reduced significantly by integrating feed systems into the gantry itself, resulting in a scaling law of n3. A completely serial assembly process will encounter time limitations as build scale increases. Automated assembly for digital materials can assemble high performance structures from discrete parts, and techniques such as built in feed systems, parallelization, and optimization of the fastening process will yield much higher throughput.

Built-up outer wall and roofing sections for double walled envelope homes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brodhead, B.

1980-01-01

A site built system that uses the inner envelope wall is described. Blocking and vertical nailers are attached to this wall and sheathed with foil faced drywall to create the envelope cavity. An outer layer of 3 1/2 in. of Expended Poly Styrene provides continuous solid insulation. The trusses are also sheathed in foil faced drywall and insulated with 5 1/2 in. of E.P.S. This effectively surrounds the building with a continuous vapor and infiltration barrier. Construction details as well as cost breakdowns are presented.

56. V2 ASSEMBLY BUILDING (BUILDING 1538): VIEW FROM NORTHEAST, WITH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

56. V-2 ASSEMBLY BUILDING (BUILDING 1538): VIEW FROM NORTHEAST, WITH MILL BUILDING IN BACKGROUND AT FAR RIGHT - White Sands Missile Range, V-2 Rocket Facilities, Near Headquarters Area, White Sands, Dona Ana County, NM

93. Neg. No. F78K, Aug 11, 1930, EXTERIORASSEMBLY BUILDING, NORTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

93. Neg. No. F-78K, Aug 11, 1930, EXTERIOR-ASSEMBLY BUILDING, NORTH SIDE, WITH TAYLOR-TRUCK-A-WAY TRUCKS AND TRAILORS - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Crystal Structure of the Japanese Encephalitis Virus Envelope Protein

DOE Office of Scientific and Technical Information (OSTI.GOV)

Luca, Vincent C.; AbiMansour, Jad; Nelson, Christopher A.

2012-03-13

Japanese encephalitis virus (JEV) is the leading global cause of viral encephalitis. The JEV envelope protein (E) facilitates cellular attachment and membrane fusion and is the primary target of neutralizing antibodies. We have determined the 2.1-{angstrom} resolution crystal structure of the JEV E ectodomain refolded from bacterial inclusion bodies. The E protein possesses the three domains characteristic of flavivirus envelopes and epitope mapping of neutralizing antibodies onto the structure reveals determinants that correspond to the domain I lateral ridge, fusion loop, domain III lateral ridge, and domain I-II hinge. While monomeric in solution, JEV E assembles as an antiparallel dimermore » in the crystal lattice organized in a highly similar fashion as seen in cryo-electron microscopy models of mature flavivirus virions. The dimer interface, however, is remarkably small and lacks many of the domain II contacts observed in other flavivirus E homodimers. In addition, uniquely conserved histidines within the JEV serocomplex suggest that pH-mediated structural transitions may be aided by lateral interactions outside the dimer interface in the icosahedral virion. Our results suggest that variation in dimer structure and stability may significantly influence the assembly, receptor interaction, and uncoating of virions.« less

Crystal structure of the Japanese encephalitis virus envelope protein.

PubMed

Luca, Vincent C; AbiMansour, Jad; Nelson, Christopher A; Fremont, Daved H

2012-02-01

Japanese encephalitis virus (JEV) is the leading global cause of viral encephalitis. The JEV envelope protein (E) facilitates cellular attachment and membrane fusion and is the primary target of neutralizing antibodies. We have determined the 2.1-Å resolution crystal structure of the JEV E ectodomain refolded from bacterial inclusion bodies. The E protein possesses the three domains characteristic of flavivirus envelopes and epitope mapping of neutralizing antibodies onto the structure reveals determinants that correspond to the domain I lateral ridge, fusion loop, domain III lateral ridge, and domain I-II hinge. While monomeric in solution, JEV E assembles as an antiparallel dimer in the crystal lattice organized in a highly similar fashion as seen in cryo-electron microscopy models of mature flavivirus virions. The dimer interface, however, is remarkably small and lacks many of the domain II contacts observed in other flavivirus E homodimers. In addition, uniquely conserved histidines within the JEV serocomplex suggest that pH-mediated structural transitions may be aided by lateral interactions outside the dimer interface in the icosahedral virion. Our results suggest that variation in dimer structure and stability may significantly influence the assembly, receptor interaction, and uncoating of virions.

FlaF is a β-sandwich protein that anchors the archaellum in the archaeal cell envelope by binding the S-layer protein

DOE PAGES

Banerjee, Ankan; Tsai, Chi -Lin; Chaudhury, Paushali; ...

2015-05-01

Archaea employ the archaellum, a type IV pilus-like nanomachine, for swimming motility. In the crenarchaeon Sulfolobus acidocaldarius, the archaellum consists of seven proteins: FlaB/X/G/F/H/I/J. FlaF is conserved and essential for archaellum assembly but no FlaF structures exist. Here, we truncated the FlaF N terminus and solved 1.5-Å and 1.65-Å resolution crystal structures of this monotopic membrane protein. Structures revealed an N-terminal α-helix and an eight-strand β-sandwich, immunoglobulin-like fold with striking similarity to S-layer proteins. Crystal structures, X-ray scattering, and mutational analyses suggest dimer assembly is needed for in vivo function. The sole cell envelope component of S. acidocaldarius is amore » paracrystalline S-layer, and FlaF specifically bound to S-layer protein, suggesting that its interaction domain is located in the pseudoperiplasm with its N-terminal helix in the membrane. From these data, FlaF may act as the previously unknown archaellum stator protein that anchors the rotating archaellum to the archaeal cell envelope.« less

Gene Targeting of Envoplakin, a Cytoskeletal Linker Protein and Precursor of the Epidermal Cornified Envelope

PubMed Central

Määttä, Arto; DiColandrea, Teresa; Groot, Karen; Watt, Fiona M.

2001-01-01

Envoplakin, a member of the plakin family of cytoskeletal linker proteins, is localized in desmosomes of stratified epithelial cells and is a component of the epidermal cornified envelope. Gene targeting in mouse embryonic stem cells was used to generate a null allele of envoplakin. No envoplakin transcripts from the targeted allele could be detected in the skin of newborn mice. Mice homozygous for the targeted allele were born in the normal Mendelian ratio and were fertile. They did not develop any discernible pathological phenotype up to the age of 1 year. The ultrastructural appearance of cornified envelopes from adult epidermis was indistinguishable between wild-type and knockout mice, and there was no evidence that the absence of envoplakin affected the subcellular distribution of periplakin or desmoplakin, two other plakins found in desmosomes. The proportion of immature cornified envelopes in the epidermis of newborn mice was greater in envoplakin-null animals than in heterozygous littermates or wild-type mice, and the envelopes had a larger surface area. This correlated with a slight delay in barrier acquisition during embryonic development. We conclude that although envoplakin is part of the scaffolding on which the cornified envelope is assembled, it is not essential for envelope formation or epidermal barrier function. PMID:11564887

DOE Office of Scientific and Technical Information (OSTI.GOV)

Levy, E.; Mullens, M.; Rath, P.

The Advanced Envelope Research effort will provide factory homebuilders with high performance, cost-effective envelope designs that can be effectively integrated into the plant production process while meeting the thermal requirements of the 2012 IECC standards. Given the affordable nature of manufactured homes, impact on first cost is a major consideration in developing new envelope technologies. This work is part of a multi-phase effort. Phase 1 identified seven envelope technologies and provided a preliminary assessment of three methods for building high performance walls. Phase 2 focused on developing viable product designs, manufacturing strategies, addressing code and structural issues, and cost analysismore » of the three selected options. An industry advisory committee helped narrow the research focus to perfecting a stud wall design with exterior continuous insulation (CI). Phase 3, completed in two stages, continued the design development effort, exploring and evaluating a range or methods for applying CI to factory built homes. The scope also included material selection, manufacturing and cost analysis, and prototyping and testing. During this phase, a home was built with CI, evaluated, and placed in service. The experience of building a mock up wall section with CI and then constructing on line a prototype home resolved important concerns about how to integrate the material into the production process. First steps were taken toward finding least expensive approaches for incorporating CI in standard factory building practices and a preliminary assessment suggested that even at this early stage the technology is attractive when viewed from a life cycle cost perspective.« less

Solution Properties of Murine Leukemia Virus Gag Protein: Differences from HIV-1 Gag

DOE Office of Scientific and Technical Information (OSTI.GOV)

Datta, Siddhartha A.K.; Zuo, Xiaobing; Clark, Patrick K.

2012-05-09

Immature retrovirus particles are assembled from the multidomain Gag protein. In these particles, the Gag proteins are arranged radially as elongated rods. We have previously characterized the properties of HIV-1 Gag in solution. In the absence of nucleic acid, HIV-1 Gag displays moderately weak interprotein interactions, existing in monomer-dimer equilibrium. Neutron scattering and hydrodynamic studies suggest that the protein is compact, and biochemical studies indicate that the two ends can approach close in three-dimensional space, implying the need for a significant conformational change during assembly. We now describe the properties of the Gag protein of Moloney murine leukemia virus (MLV),more » a gammaretrovirus. We found that this protein is very different from HIV-1 Gag: it has much weaker protein-protein interaction and is predominantly monomeric in solution. This has allowed us to study the protein by small-angle X-ray scattering and to build a low-resolution molecular envelope for the protein. We found that MLV Gag is extended in solution, with an axial ratio of {approx}7, comparable to its dimensions in immature particles. Mutational analysis suggests that runs of prolines in its matrix and p12 domains and the highly charged stretch at the C terminus of its capsid domain all contribute to this extended conformation. These differences between MLV Gag and HIV-1 Gag and their implications for retroviral assembly are discussed.« less

Spreadsheet Assessment Tool v. 2.4

DOE Office of Scientific and Technical Information (OSTI.GOV)

Allen, David J.; Martinez, Ruben

2016-03-03

The Spreadsheet Assessment Tool (SAT) is an easy to use, blast assessment tool that is intended to estimate the potential risk due to an explosive attack on a blood irradiator. The estimation of risk is based on the methodology, assumptions, and results of a detailed blast effects assessment study that is summarized in Sandia National Laboratories Technical Report SAND2015-6166. Risk as defined in the report and as used in the SAT is: "The potential risk of creating an air blast-induced vent opening at a buildings envelope surface". Vent openings can be created at a buildings envelope through the failure ofmore » an exterior building component—like a wall, window, or door—due to an explosive sabotage of an irradiator within the building. To estimate risk, the tool requires that users obtain and input information pertaining to the building's characteristics and the irradiator location. The tool also suggests several prescriptive mitigation strategies that can be considered to reduce risk. Given the variability in civilian building construction practices, the input parameters used by this tool may not apply to all buildings being assessed. The tool should not be used as a substitute for engineering judgment. The tool is intended for assessment purposes only.« less

A Fast Evaluation Method for Energy Building Consumption Based on the Design of Experiments

NASA Astrophysics Data System (ADS)

Belahya, Hocine; Boubekri, Abdelghani; Kriker, Abdelouahed

2017-08-01

Building sector is one of the effective consumer energy by 42% in Algeria. The need for energy has continued to grow, in inordinate way, due to lack of legislation on energy performance in this large consumer sector. Another reason is the simultaneous change of users’ requirements to maintain their comfort, especially summer in dry lands and parts of southern Algeria, where the town of Ouargla presents a typical example which leads to a large amount of electricity consumption through the use of air conditioning. In order to achieve a high performance envelope of the building, an optimization of major parameters building envelope is required, using design of experiments (DOE), can determine the most effective parameters and eliminate the less importance. The study building is often complex and time consuming due to the large number of parameters to consider. This study focuses on reducing the computing time and determines the major parameters of building energy consumption, such as area of building, factor shape, orientation, ration walls to windows …etc to make some proposal models in order to minimize the seasonal energy consumption due to air conditioning needs.

Measuring Thermal Performance of Building Envelopes: Nine Case Studies,

DTIC Science & Technology

1985-03-01

inch P/B = 11.36, present worth factor for an es- of expanded polystyrene insulation) to the build- calating series for a 15-year period ing exterior...inch of The one MCA building roof we measured was a expanded polystyrene at R-3.6 per inch. Where the cathedral system with a sloped built-up roofing

Study of an experimental methodology for thermal properties diagnostic of building envelop

NASA Astrophysics Data System (ADS)

Yang, Yingying; Sempy, Alain; Vogt Wu, Tingting; Sommier, Alain; Dumoulin, Jean; Batsale, Jean Christophe

2017-04-01

The building envelope plays a critical role in determining levels of comfort and building efficiency. Its real thermal properties characterization is of major interest to be able to diagnose energy efficiency performance of buildings (new construction and retrofitted existing old building). Research and development on a possible methodology for energy diagnostic of the building envelop is a hot topic and necessary trend. Many kinds of sensors and instruments are used for the studies. The application of infrared (IR) thermography in non-destructive evaluation has been widely employed for qualitative evaluations for building diagnostics; meanwhile, the IR thermography technology also has a large potentiality for the evaluation of the thermal characteristics of the building envelope. Some promising recent research studies have been carried out with such contactless measurement technique. Nevertheless, research efforts are still required for in situ measurements under natural environmental conditions. In order to develop new solutions for non-intrusive evaluation of local thermal performance, enabling quantitative assessment of thermal properties of buildings and materials, experiments were carried out on a multi-layer pratical scale wall fixed on a caisson placed in a climatic chamber. Six halogen lamps (1.5 kW for each lamp) placed in front of objective wall were used to emulate sunny conditions. The radiative heat flux emitted was monitored and modulated with time according to typical weather data set encountered in France. Both steady state and transient regime heat transfer were studied during these experiments. Contact sensors (thermocouples, heat flux meters, Peltier sensors) and non-contact sensors (thermal IR camera, pyranometer) were used to measure the temperatures and heat flux density evolution. It has to be noticed that the Peltier sensors have been tuned and used with a specific processing to set them compliant for heat flux density measurements. The measured data from different sensors were analysed and compared. The emissivity of wall surface and treated sensor surfaces were evaluated by using an IR camera with an adapted post-processing. Then, convective and radiative heat fluxes, at wall level, were estimated. Finally, the wall thermal properties can be calculated by using the measured temperatures and estimated heat fluxes using a dedicated thermal quadrupoles heat transfer model and an inverse method. This study aims at providing some guidelines for the choice of sensors, measurements protocol and adapted inverse model to be tested in real conditions on pilot situ scale. Aknowledgments : The Authors are very grateful to H2020 Built2Spec project for supporting this work.

Intelligent Facades for High Performance Green Buildings

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dyson, Anna

Progress Towards Net-Zero and Net-Positive-Energy Commercial Buildings and Urban Districts Through Intelligent Building Envelope Strategies Previous research and development of intelligent facades systems has been limited in their contribution towards national goals for achieving on-site net zero buildings, because this R&D has failed to couple the many qualitative requirements of building envelopes such as the provision of daylighting, access to exterior views, satisfying aesthetic and cultural characteristics, with the quantitative metrics of energy harvesting, storage and redistribution. To achieve energy self-sufficiency from on-site solar resources, building envelopes can and must address this gamut of concerns simultaneously. With this project, wemore » have undertaken a high-performance building integrated combined-heat and power concentrating photovoltaic system with high temperature thermal capture, storage and transport towards multiple applications (BICPV/T). The critical contribution we are offering with the Integrated Concentrating Solar Façade (ICSF) is conceived to improve daylighting quality for improved health of occupants and mitigate solar heat gain while maximally capturing and transferring onsite solar energy. The ICSF accomplishes this multi-functionality by intercepting only the direct-normal component of solar energy (which is responsible for elevated cooling loads) thereby transforming a previously problematic source of energy into a high quality resource that can be applied to building demands such as heating, cooling, dehumidification, domestic hot water, and possible further augmentation of electrical generation through organic Rankine cycles. With the ICSF technology, our team is addressing the global challenge in transitioning commercial and residential building stock towards on-site clean energy self-sufficiency, by fully integrating innovative environmental control systems strategies within an intelligent and responsively dynamic building envelope. The advantage of being able to use the entire solar spectrum for active and passive benefits, along with the potential savings of avoiding transmission losses through direct current (DC) transfer to all buildings systems directly from the site of solar conversion, gives the system a compounded economic viability within the commercial and institutional building markets. With a team that spans multiple stakeholders across disparate industries, from CPV to A&E partners that are responsible for the design and development of District and Regional Scale Urban Development, this project demonstrates that integrating utility-scale high efficiency CPV installations with urban and suburban environments is both viable and desirable within the marketplace. The historical schism between utility scale CPV and BIPV has been one of differing scale and cultures. There is no technical reason why utility-scale CPV cannot be located within urban embedded district scale sites of energy harvesting. New models for leasing large areas of district scale roofs and facades are emerging, such that the model for utility scale energy harvesting can be reconciled to commercial and public scale building sites and campuses. This consortium is designed to unite utility scale solar harvesting into building applications for smart grid development.« less

95. JOB NO. 1347F, SHEET 1, 1927/1931, PLOT PLAN, ASSEMBLY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

95. JOB NO. 1347-F, SHEET 1, 1927/1931, PLOT PLAN, ASSEMBLY BUILDING FOR FORD MOTOR COMPANY, LONG BEACH, CALIFORNIA - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates

PubMed Central

Franz, Cerstin; Askjaer, Peter; Antonin, Wolfram; Iglesias, Carmen López; Haselmann, Uta; Schelder, Malgorzata; de Marco, Ario; Wilm, Matthias; Antony, Claude; Mattaj, Iain W

2005-01-01

Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segregation at anaphase. Nup155 depletion inhibited accumulation of nucleoporins at the nuclear periphery, including those recruited to chromatin early in NE formation. Electron microscopy analysis revealed that Nup155 is also required for the formation of a continuous nuclear membrane in vivo and in vitro. Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs. PMID:16193066

One piece microwave container screens for electrodeless lamps

DOEpatents

Turner, Brian; Ury, Michael

1998-01-01

A microwave powered electrodeless lamp includes an improved screen unit having mesh and solid sections with an internal reflector to reflect light into a light-transmitting chamber defined in the lamp microwave cavity by the reflector and the mesh section. A discharge envelope of a bulb is disposed in the light-transmitting chamber. Light emitted from the envelope is prevented by the reflector from entering the cavity portion bounded by the solid section of the screen. Replacing mesh material by solid metal material as part of the screen unit significantly reduces leakage of microwave energy from the lamp. The solid section has multiple compliant fingers defined therein for engaging the periphery of a flange on the waveguide unit so that a hose clamp can easily secure the screen to the assembly. Screen units of this type having different mesh section configurations can be interchanged in the lamp assembly to produce different respective illumination patterns.

Solvent mediated hybrid 2D materials: black phosphorus - graphene heterostructured building blocks assembled for sodium ion batteries.

PubMed

Li, Mengya; Muralidharan, Nitin; Moyer, Kathleen; Pint, Cary L

2018-06-07

Here we demonstrate the broad capability to exploit interactions at different length scales in 2D materials to prepare macroscopic functional materials containing hybrid black phosphorus/graphene (BP/G) heterostructured building blocks. First, heterostructured 2D building blocks are self-assembled during co-exfoliation in the solution phase based on electrostatic attraction of different 2D materials. Second, electrophoretic deposition is used as a tool to assemble these building blocks into macroscopic films containing these self-assembled 2D heterostructures. Characterization of deposits formed using this technique elucidates the presence of stacked and sandwiched 2D heterostructures, and zeta potential measurements confirm the mechanistic interactions driving this assembly. Building on the exceptional sodium alloying capacity of BP, these materials were demonstrated as superior binder-free and additive-free anodes for sodium batteries with specific discharge capacity of 2365 mA h gP-1 and long stable cycling duration. This study demonstrates how controllable co-processing of 2D materials can enable material control for stacking and building block assembly relevant to broad future applications of 2D materials.

37 CFR 360.4 - Compliance with statutory dates.

Code of Federal Regulations, 2012 CFR

2012-07-01

... to the Copyright Office Public Information Office, in the James Madison Memorial Building, Room LM... Congress, James Madison Memorial Building, 101 Independence Avenue, SE., Washington, DC 20559-6000. Claims... envelope must be addressed as follows: Copyright Royalty Board, Library of Congress, James Madison Memorial...

37 CFR 360.13 - Compliance with statutory dates.

Code of Federal Regulations, 2013 CFR

2013-07-01

... delivered to the Copyright Office Public Information Office, in the James Madison Memorial Building, Room LM... Congress, James Madison Memorial Building, 101 Independence Avenue, SE., Washington, DC 20559-6000. Claims... envelope must be addressed as follows: Copyright Royalty Board, Library of Congress, James Madison Memorial...

37 CFR 360.13 - Compliance with statutory dates.

Code of Federal Regulations, 2012 CFR

2012-07-01

... delivered to the Copyright Office Public Information Office, in the James Madison Memorial Building, Room LM... Congress, James Madison Memorial Building, 101 Independence Avenue, SE., Washington, DC 20559-6000. Claims... envelope must be addressed as follows: Copyright Royalty Board, Library of Congress, James Madison Memorial...

37 CFR 360.13 - Compliance with statutory dates.

Code of Federal Regulations, 2014 CFR

2014-07-01

... delivered to the Copyright Office Public Information Office, in the James Madison Memorial Building, Room LM... Congress, James Madison Memorial Building, 101 Independence Avenue, SE., Washington, DC 20559-6000. Claims... envelope must be addressed as follows: Copyright Royalty Board, Library of Congress, James Madison Memorial...

37 CFR 360.4 - Compliance with statutory dates.

Code of Federal Regulations, 2013 CFR

2013-07-01

... to the Copyright Office Public Information Office, in the James Madison Memorial Building, Room LM... Congress, James Madison Memorial Building, 101 Independence Avenue, SE., Washington, DC 20559-6000. Claims... envelope must be addressed as follows: Copyright Royalty Board, Library of Congress, James Madison Memorial...

37 CFR 360.4 - Compliance with statutory dates.

Code of Federal Regulations, 2014 CFR

2014-07-01

... to the Copyright Office Public Information Office, in the James Madison Memorial Building, Room LM... Congress, James Madison Memorial Building, 101 Independence Avenue, SE., Washington, DC 20559-6000. Claims... envelope must be addressed as follows: Copyright Royalty Board, Library of Congress, James Madison Memorial...

Improved Peak Detection and Deconvolution of Native Electrospray Mass Spectra from Large Protein Complexes.

PubMed

Lu, Jonathan; Trnka, Michael J; Roh, Soung-Hun; Robinson, Philip J J; Shiau, Carrie; Fujimori, Danica Galonic; Chiu, Wah; Burlingame, Alma L; Guan, Shenheng

2015-12-01

Native electrospray-ionization mass spectrometry (native MS) measures biomolecules under conditions that preserve most aspects of protein tertiary and quaternary structure, enabling direct characterization of large intact protein assemblies. However, native spectra derived from these assemblies are often partially obscured by low signal-to-noise as well as broad peak shapes because of residual solvation and adduction after the electrospray process. The wide peak widths together with the fact that sequential charge state series from highly charged ions are closely spaced means that native spectra containing multiple species often suffer from high degrees of peak overlap or else contain highly interleaved charge envelopes. This situation presents a challenge for peak detection, correct charge state and charge envelope assignment, and ultimately extraction of the relevant underlying mass values of the noncovalent assemblages being investigated. In this report, we describe a comprehensive algorithm developed for addressing peak detection, peak overlap, and charge state assignment in native mass spectra, called PeakSeeker. Overlapped peaks are detected by examination of the second derivative of the raw mass spectrum. Charge state distributions of the molecular species are determined by fitting linear combinations of charge envelopes to the overall experimental mass spectrum. This software is capable of deconvoluting heterogeneous, complex, and noisy native mass spectra of large protein assemblies as demonstrated by analysis of (1) synthetic mononucleosomes containing severely overlapping peaks, (2) an RNA polymerase II/α-amanitin complex with many closely interleaved ion signals, and (3) human TriC complex containing high levels of background noise. Graphical Abstract ᅟ.

Development of low thermal conductivity brick using rice husk, corn cob and waste tea in clay brick manufacturing

NASA Astrophysics Data System (ADS)

Saman, Nor Sarwani Mat; Deraman, Rafikullah; Hamzah, Mohamad Hazmi

2017-12-01

The consumption of energy for cooling the indoor environment of buildings in Malaysia is high and mostly related to poor thermal performance of the building envelope. It is evident that reducing energy consumption of buildings has become vital, taking into considerations the limitation of conventional energy resources and the adverse effects associated with the use of such type of energy on the environment. Therefore, selecting the proper thermal properties of a building envelope play a major role in determining the energy consumption patterns and comfort conditions in enclosed spaces. The objective of this study is to investigate the potential application of rice husk (RH), corn cob (CC) and waste tea (WT) as an additive agent in a fired clay brick manufacturing to produce an improved thermal conductivity of final brick product. In the execution of this study, these agricultural wastes were mixed together with clay soil in different percentages, ranging from 0 %, 2.5 %, 5 %, 7.5 % and 10 % by weight. Physical and mechanical properties including soil physical properties, density, shrinkage, water absorption, compressive strength as well as thermal conductivity were measured, reported and discussed in accordance with BS 1377: Part 2: 1990, BS 3921: 1985, MS 76: 1972: Part 2 and ASTM C 518. The results show that RH at 7.5 % is the most effective combination to achieve low thermal conductivity of fired clay brick. This finding suggests that RH waste is a potentially good additive material to be used for thermal properties enhancement of the building envelope.

Passive solar/Earth sheltered office/dormitory cooling season thermal performance

NASA Astrophysics Data System (ADS)

Christian, J.

1984-06-01

Continuous detailed hourly thermal performance measurements were taken since February 1982 in and around an occupied, underground, 4000 ft(2) office/dormitory building at the Oak Ridge National Laboratory in Oak Ridge, Tennessee. This building has a number of energy saving features which were analyzed relative to their performance in a southeastern US climate and with respect to overall commercial building performance. Cooling season performance is documented, as well as effects of earth constact, interior thermal mass, an economizer cycle and interface of an efficient building envelope with a central three-ton heat pump. The Joint Institute Dormitory obtains a cooling energy savings of about 30% compared with an energy-efficient, above-grade structure and has the potential to save as much as 50%. The proper instllation of the overhand, interior thermal mass, massive supply duct system, and earth contact team up to prevent summertime overheating. From May through September, this building cost a total of $300 (at 5.7) cents/kWh) to cool and ventilate 24 hours per day. Besides thermal performance of the building envelope, extensive comfort data was taken illustrating that at least 90% of the occupants are comfortable all of the time according to the PMV measurements.

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA, John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA, John F. Kennedy Space Center, Florida. Drawing 79K05424, Seelye Stevenson Value & Knecht, March, 1975. SITE WORK, GENERAL AREA PLAN. Sheet 8 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING. NASA John F. Kennedy ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING. NASA John F. Kennedy Space Center, Florida. File Number 79K06740, NASA, November 1975. SPACE & WEIGHT ALLOCATION, ORBITER PATH IN TRANSFER AISLE. Sheet 6 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Country Report on Building Energy Codes in Australia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shui, Bin; Evans, Meredydd; Somasundaram, Sriram

2009-04-02

This report is part of a series of reports on building energy efficiency codes in countries associated with the Asian Pacific Partnership (APP) - Australia, South Korea, Japan, China, India, and the United States of America (U.S.). This reports gives an overview of the development of building energy codes in Australia, including national energy policies related to building energy codes, history of building energy codes, recent national projects and activities to promote building energy codes. The report also provides a review of current building energy codes (such as building envelope, HVAC, and lighting) for commercial and residential buildings in Australia.

Self-Assembly: How Nature Builds

ERIC Educational Resources Information Center

Jones, M. Gail; Falvo, Michael R.; Broadwell, Bethany; Dotger, Sharon

2006-01-01

Self-assembly or spontaneous assembly is a process in which materials build themselves without assistance. This process plays a central role in the construction of biological structures and materials such as cells, viruses, and bone, and also in abiotic processes like phase transitions and crystal formation. The principles of self-assembly help…

A Point Mutation in the N-Terminal Amphipathic Helix α0 in NS3 Promotes Hepatitis C Virus Assembly by Altering Core Localization to the Endoplasmic Reticulum and Facilitating Virus Budding.

PubMed

Yan, Yu; He, Ying; Boson, Bertrand; Wang, Xuesong; Cosset, François-Loïc; Zhong, Jin

2017-03-15

The assembly of hepatitis C virus (HCV), a complicated process in which many viral and cellular factors are involved, has not been thoroughly deciphered. NS3 is a multifunctional protein that contains an N-terminal amphipathic α helix (designated helix α 0 ), which is crucial for the membrane association and stability of NS3 protein, followed by a serine protease domain and a C-terminal helicase/NTPase domain. NS3 participates in HCV assembly likely through its C-terminal helicase domain, in which all reported adaptive mutations enhancing virion assembly reside. In this study, we determined that the N-terminal helix α 0 of NS3 may contribute to HCV assembly. We identified a single mutation from methionine to threonine at amino acid position 21 (M21T) in helix α 0 , which significantly promoted viral production while having no apparent effect on the membrane association and protease activity of NS3. Subsequent analyses demonstrated that the M21T mutation did not affect HCV genome replication but rather promoted virion assembly. Further study revealed a shift in the subcellular localization of core protein from lipid droplets (LD) to the endoplasmic reticulum (ER). Finally, we showed that the M21T mutation increased the colocalization of core proteins and viral envelope proteins, leading to a more efficient envelopment of viral nucleocapsids. Collectively, the results of our study revealed a new function of NS3 helix α 0 and aid understanding of the role of NS3 in HCV virion morphogenesis. IMPORTANCE HCV NS3 protein possesses the protease activity in its N-terminal domain and the helicase activity in its C-terminal domain. The role of NS3 in virus assembly has been mainly attributed to its helicase domain, because all adaptive mutations enhancing progeny virus production are found to be within this domain. Our study identified, for the first time to our knowledge, an adaptive mutation within the N-terminal helix α 0 domain of NS3 that significantly enhanced virus assembly while having no effect on viral genome replication. The mechanistic studies suggested that this mutation promoted the relocation of core proteins from LD to the ER, leading to a more efficient envelopment of viral nucleocapsids. Our results revealed a possible new function of helix α 0 in the HCV life cycle and provided new clues to understanding the molecular mechanisms for the action of NS3 in HCV assembly. Copyright © 2017 American Society for Microbiology.

Smart Energy Choices Free Up Dollars for Capital Improvements.

ERIC Educational Resources Information Center

Ritchey, David

2003-01-01

Describes several ways to design or renovate school building to save thousand of dollars of energy costs. Considers site design, energy-efficient building envelope, renewable energy systems, lighting and electrical systems, mechanical and ventilation systems, water conservation, and transportation. Describes how to obtain information about the…

34. SOUTHWEST CORNER OF BUILDING 233 (MISSILE ASSEMBLY SHOP) IN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

34. SOUTHWEST CORNER OF BUILDING 233 (MISSILE ASSEMBLY SHOP) IN ASSEMBLY AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

ARCHITECTURAL, 777M, PHYSICS ASSEMBLY LABORATORY BUILDING, EQUIPMENT ARRANGEMENT – SECTIONS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

ARCHITECTURAL, 777-M, PHYSICS ASSEMBLY LABORATORY BUILDING, EQUIPMENT ARRANGEMENT – SECTIONS “B” AND “C” (W157132) - Physics Assembly Laboratory, Area A/M, Savannah River Site, Aiken, Aiken County, SC

13. VIEW NORTHEAST, BUILDING 12 INTERIOR, WIND TUNNEL FAN ASSEMBLY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. VIEW NORTHEAST, BUILDING 12 INTERIOR, WIND TUNNEL FAN ASSEMBLY - Naval Surface Warfare Center, Transonic Wind Tunnel Building, Bounded by Clara Barton Parkway & McArthur Boulevard, Silver Spring, Montgomery County, MD

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cheng, Meng -Dawn; Allman, Steve L.; Graham, David E.

Building envelope, such as a roof, is the interface between a building structure and the environment. Understanding of the physics of microbial interactions with the building envelope is limited. In addition to the natural weathering, microorganisms and airborne particulate matter that attach to a cool roof tend to reduce the roof reflectance over time, compromising the energy efficiency advantages of the reflective coating designs. We applied microbial ecology analysis to identify the natural communities present on the exposed coatings and investigated the reduction kinetics of the surface reflectance upon the introduction of a defined mixture of both photoautotrophic and heterotrophicmore » microorganisms representing the natural communities. The result are (1) reflectance degradation by microbial communities follows a first-order kinetic relationship and (2) more than 50% of degradation from the initial reflectance value can be caused by microbial species alone in much less time than 3 years required by the current standard ENERGY STAR® test methods.« less

Low-Cost Phase Change Material for Building Envelopes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abhari, Ramin

2015-08-06

A low-cost PCM process consisting of conversion of fats and oils to PCM-range paraffins, and subsequent “encapsulation” of the paraffin using conventional plastic compounding/pelletizing equipment was demonstrated. The PCM pellets produced were field-tested in a building envelope application. This involved combining the PCM pellets with cellulose insulation, whereby 33% reduction in peak heat flux and 12% reduction in heat gain was observed (average summertime performance). The selling price of the PCM pellets produced according to this low-cost process is expected to be in the $1.50-$3.00/lb range, compared to current encapsulated PCM price of about $7.00/lb. Whole-building simulations using corresponding PCMmore » thermal analysis data suggest a payback time of 8 to 16 years (at current energy prices) for an attic insulation retrofit project in the Phoenix climate area.« less

Laboratory testing of a building envelope segment based on cellular concrete

NASA Astrophysics Data System (ADS)

Fořt, Jan; Pavlík, Zbyšek; Černý, Robert

2016-07-01

Hygrothermal performance of a building envelope based on cellular concrete blocks is studied in the paper. Simultaneously, the strain fields induced by the heat and moisture changes are monitored. The studied wall is exposed to the climatic load corresponding to the winter climatic conditions of the moderate year for Prague. The winter climatic exposure is chosen in order to simulate the critical conditions of the building structure from the point of view of material performance and temperature and humidity loading. The evaluation of hygrothermal performance of a researched wall is done on the basis of relative humidity and temperature profiles measured along the cross section of the cellular concrete blocks. Strain gauges are fixed on the wall surface in expected orientation of the blocks expansion. The obtained results show a good hygrothermal function of the analyzed cellular concrete wall and its insignificant strain.

Surface reflectance degradation by microbial communities

DOE PAGES

Cheng, Meng -Dawn; Allman, Steve L.; Graham, David E.; ...

2015-11-05

Building envelope, such as a roof, is the interface between a building structure and the environment. Understanding of the physics of microbial interactions with the building envelope is limited. In addition to the natural weathering, microorganisms and airborne particulate matter that attach to a cool roof tend to reduce the roof reflectance over time, compromising the energy efficiency advantages of the reflective coating designs. We applied microbial ecology analysis to identify the natural communities present on the exposed coatings and investigated the reduction kinetics of the surface reflectance upon the introduction of a defined mixture of both photoautotrophic and heterotrophicmore » microorganisms representing the natural communities. The result are (1) reflectance degradation by microbial communities follows a first-order kinetic relationship and (2) more than 50% of degradation from the initial reflectance value can be caused by microbial species alone in much less time than 3 years required by the current standard ENERGY STAR® test methods.« less

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. ALL PLATFORMS-ARCHITECTURAL, GENERAL ARRANGEMENT, EAST-WEST ELEVATIONS. Sheet 12 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Vehicle Assembly Building (VAB)

NASA Image and Video Library

2017-09-27

NASA's Vehicle Assembly Building at Kennedy Space Center in Florida was used to assemble and house American-crewed launch vehicles from 1968 to 2011. AT 3,684,883 cubic meters, it is one of the largest buildings in the world by volume. Inside the facility, High Bay 3 is being upgraded and modified to support processing of the agency's Space Launch System rocket and Orion spacecraft.

Spatiotemporal Regulation of Nuclear Transport Machinery and Microtubule Organization

PubMed Central

Okada, Naoyuki; Sato, Masamitsu

2015-01-01

Spindle microtubules capture and segregate chromosomes and, therefore, their assembly is an essential event in mitosis. To carry out their mission, many key players for microtubule formation need to be strictly orchestrated. Particularly, proteins that assemble the spindle need to be translocated at appropriate sites during mitosis. A small GTPase (hydrolase enzyme of guanosine triphosphate), Ran, controls this translocation. Ran plays many roles in many cellular events: nucleocytoplasmic shuttling through the nuclear envelope, assembly of the mitotic spindle, and reorganization of the nuclear envelope at the mitotic exit. Although these events are seemingly distinct, recent studies demonstrate that the mechanisms underlying these phenomena are substantially the same as explained by molecular interplay of the master regulator Ran, the transport factor importin, and its cargo proteins. Our review focuses on how the transport machinery regulates mitotic progression of cells. We summarize translocation mechanisms governed by Ran and its regulatory proteins, and particularly focus on Ran-GTP targets in fission yeast that promote spindle formation. We also discuss the coordination of the spatial and temporal regulation of proteins from the viewpoint of transport machinery. We propose that the transport machinery is an essential key that couples the spatial and temporal events in cells. PMID:26308057

Endoplasmic Reticulum-Golgi Intermediate Compartment Membranes and Vimentin Filaments Participate in Vaccinia Virus Assembly

PubMed Central

Risco, Cristina; Rodríguez, Juan R.; López-Iglesias, Carmen; Carrascosa, José L.; Esteban, Mariano; Rodríguez, Dolores

2002-01-01

Vaccinia virus (VV) has a complex morphogenetic pathway whose first steps are poorly characterized. We have studied the early phase of VV assembly, when viral factories and spherical immature viruses (IVs) form in the cytoplasm of the infected cell. After freeze-substitution numerous cellular elements are detected around assembling viruses: membranes, ribosomes, microtubules, filaments, and unidentified structures. A double membrane is clearly resolved in the VV envelope for the first time, and freeze fracture reveals groups of tubules interacting laterally on the surface of the viroplasm foci. These data strongly support the hypothesis of a cellular tubulovesicular compartment, related to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC), as the origin of the first VV envelope. Moreover, the cytoskeletal vimentin intermediate filaments are found around viral factories and inside the viroplasm foci, where vimentin and the VV core protein p39 colocalize in the areas where crescents protrude. Confocal microscopy showed that ERGIC elements and vimentin filaments concentrate in the viral factories. We propose that modified cellular ERGIC membranes and vimentin intermediate filaments act coordinately in the construction of viral factories and the first VV form through a unique mechanism of viral morphogenesis from cellular elements. PMID:11799179

36. CONTEXT VIEW LOOKING SOUTHWEST OF BUILDING 233 (MISSLE ASSEMBLY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

36. CONTEXT VIEW LOOKING SOUTHWEST OF BUILDING 233 (MISSLE ASSEMBLY SHOP) IN ASSEMBLY AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

35. EAST FRONT ELEVATION OF BUILDING 233 (MISSLE ASSEMBLY SHOP) ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. EAST FRONT ELEVATION OF BUILDING 233 (MISSLE ASSEMBLY SHOP) IN ASSEMBLY AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

16. Exterior of assembly building (mold loft #2) used for ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. Exterior of assembly building (mold loft #2) used for runabout production, constructed CA. 1932. Most of the production run boats were assembled here. - Barbour Boat Works, Tryon Palace Drive, New Bern, Craven County, NC

SynTrack: DNA Assembly Workflow Management (SynTrack) v2.0.1

DOE Office of Scientific and Technical Information (OSTI.GOV)

MENG, XIANWEI; SIMIRENKO, LISA

2016-12-01

SynTrack is a dynamic, workflow-driven data management system that tracks the DNA build process: Management of the hierarchical relationships of the DNA fragments; Monitoring of process tasks for the assembly of multiple DNA fragments into final constructs; Creations of vendor order forms with selectable building blocks. Organizing plate layouts barcodes for vendor/pcr/fusion/chewback/bioassay/glycerol/master plate maps (default/condensed); Creating or updating Pre-Assembly/Assembly process workflows with selected building blocks; Generating Echo pooling instructions based on plate maps; Tracking of building block orders, received and final assembled for delivering; Bulk updating of colony or PCR amplification information, fusion PCR and chewback results; Updating with QA/QCmore » outcome with .csv & .xlsx template files; Re-work assembly workflow enabled before and after sequencing validation; and Tracking of plate/well data changes and status updates and reporting of master plate status with QC outcomes.« less

Venture from the Interior-Herpesvirus pUL31 Escorts Capsids from Nucleoplasmic Replication Compartments to Sites of Primary Envelopment at the Inner Nuclear Membrane.

PubMed

Bailer, Susanne M.

2017-11-25

Herpesviral capsid assembly is initiated in the nucleoplasm of the infected cell. Size constraints require that newly formed viral nucleocapsids leave the nucleus by an evolutionarily conserved vescular transport mechanism called nuclear egress. Mature capsids released from the nucleoplasm are engaged in a membrane-mediated budding process, composed of primary envelopment at the inner nuclear membrane and de-envelopment at the outer nuclear membrane. Once in the cytoplasm, the capsids receive their secondary envelope for maturation into infectious virions. Two viral proteins conserved throughout the herpesvirus family, the integral membrane protein pUL34 and the phosphoprotein pUL31, form the nuclear egress complex required for capsid transport from the infected nucleus to the cytoplasm. Formation of the nuclear egress complex results in budding of membrane vesicles revealing its function as minimal virus-encoded membrane budding and scission machinery. The recent structural analysis unraveled details of the heterodimeric nuclear egress complex and the hexagonal coat it forms at the inside of budding vesicles to drive primary envelopment. With this review, I would like to present the capsid-escort-model where pUL31 associates with capsids in nucleoplasmic replication compartments for escort to sites of primary envelopment thereby coupling capsid maturation and nuclear egress.

Kinetic Super-Resolution Long-Wave Infrared (KSR LWIR) Thermography Diagnostic for Building Envelopes: Camp Lejeune, NC

DTIC Science & Technology

2015-08-18

of Defense (DoD) to achieve cost-effective energy efficiency at much greater scale than other commercially available techniques of measuring energy...recommends specific energy conservation measures (ECMs), and quantifies significant potential return on investment. ERDC/CERL TR-15-18 iii...effective energy efficiency at much greater scale than other commercially available techniques of measuring energy loss due to envelope inefficien- cies

36 CFR § 702.9 - Inspection of property.

Code of Federal Regulations, 2013 CFR

2013-07-01

....9 Section § 702.9 Parks, Forests, and Public Property LIBRARY OF CONGRESS CONDUCT ON LIBRARY PREMISES § 702.9 Inspection of property. (a) Individuals entering Library buildings do so with the... envelopes, packages, and office equipment may be inspected. (b) Upon entering the Library buildings...

19. Launch Area, general view of Missile Assembly Building and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. Launch Area, general view of Missile Assembly Building and Generator Building VIEW SOUTHWEST - NIKE Missile Battery PR-79, Launch Area, East Windsor Road south of State Route 101, Foster, Providence County, RI

Feasibility and Scaling of Composite Based Additive Manufacturing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nuttall, David; Chen, Xun; Kunc, Vlastimil

2016-04-27

Engineers and Researchers at Oak Ridge National Lab s Manufacturing Demonstration Facility (ORNL MDF) collaborated with Impossible Objects (IO) in the characterization of PEEK infused carbon fiber mat manufactured by means of CBAM composite-based additive manufacturing, a first generation assembly methodology developed by Robert Swartz, Chairman, Founder, and CTO of Impossible Objects.[1] The first phase of this project focused on demonstration of CBAM for composite tooling. The outlined steps focused on selecting an appropriate shape that fit the current machine s build envelope, characterized the resulting form, and presented next steps for transitioning to a Phase II CRADA agreement. Phasemore » I of collaborative research and development agreement NFE-15-05698 was initiated in April of 2015 with an introduction to Impossible Objects, and concluded in March of 2016 with a visitation to Impossible Objects headquarters in Chicago, IL. Phase II as discussed herein is under consideration by Impossible Objects as of this writing.« less

Vision-based building energy diagnostics and retrofit analysis using 3D thermography and building information modeling

NASA Astrophysics Data System (ADS)

Ham, Youngjib

The emerging energy crisis in the building sector and the legislative measures on improving energy efficiency are steering the construction industry towards adopting new energy efficient design concepts and construction methods that decrease the overall energy loads. However, the problems of energy efficiency are not only limited to the design and construction of new buildings. Today, a significant amount of input energy in existing buildings is still being wasted during the operational phase. One primary source of the energy waste is attributed to unnecessary heat flows through building envelopes during hot and cold seasons. This inefficiency increases the operational frequency of heating and cooling systems to keep the desired thermal comfort of building occupants, and ultimately results in excessive energy use. Improving thermal performance of building envelopes can reduce the energy consumption required for space conditioning and in turn provide building occupants with an optimal thermal comfort at a lower energy cost. In this sense, energy diagnostics and retrofit analysis for existing building envelopes are key enablers for improving energy efficiency. Since proper retrofit decisions of existing buildings directly translate into energy cost saving in the future, building practitioners are increasingly interested in methods for reliable identification of potential performance problems so that they can take timely corrective actions. However, sensing what and where energy problems are emerging or are likely to emerge and then analyzing how the problems influence the energy consumption are not trivial tasks. The overarching goal of this dissertation focuses on understanding the gaps in knowledge in methods for building energy diagnostics and retrofit analysis, and filling these gaps by devising a new method for multi-modal visual sensing and analytics using thermography and Building Information Modeling (BIM). First, to address the challenges in scaling and localization issues of 2D thermal image-based inspection, a new computer vision-based method is presented for automated 3D spatio-thermal modeling of building environments from images and localizing the thermal images into the 3D reconstructed scenes, which helps better characterize the as-is condition of existing buildings in 3D. By using these models, auditors can conduct virtual walk-through in buildings and explore the as-is condition of building geometry and the associated thermal conditions in 3D. Second, to address the challenges in qualitative and subjective interpretation of visual data, a new model-based method is presented to convert the 3D thermal profiles of building environments into their associated energy performance metrics. More specifically, the Energy Performance Augmented Reality (EPAR) models are formed which integrate the actual 3D spatio-thermal models ('as-is') with energy performance benchmarks ('as-designed') in 3D. In the EPAR models, the presence and location of potential energy problems in building environments are inferred based on performance deviations. The as-is thermal resistances of the building assemblies are also calculated at the level of mesh vertex in 3D. Then, based on the historical weather data reflecting energy load for space conditioning, the amount of heat transfer that can be saved by improving the as-is thermal resistances of the defective areas to the recommended level is calculated, and the equivalent energy cost for this saving is estimated. The outcome provides building practitioners with unique information that can facilitate energy efficient retrofit decision-makings. This is a major departure from offhand calculations that are based on historical cost data of industry best practices. Finally, to improve the reliability of BIM-based energy performance modeling and analysis for existing buildings, a new model-based automated method is presented to map actual thermal resistance measurements at the level of 3D vertexes to the associated BIM elements and update their corresponding thermal properties in the gbXML schema. By reflecting the as-is building condition in the BIM-based energy modeling process, this method bridges over the gap between the architectural information in the as-designed BIM and the as-is building condition for accurate energy performance analysis. The performance of each method was validated on ten case studies from interiors and exteriors of existing residential and instructional buildings in IL and VA. The extensive experimental results show the promise of the proposed methods in addressing the fundamental challenges of (1) visual sensing : scaling 2D visual assessments to real-world building environments and localizing energy problems; (2) analytics: subjective and qualitative assessments; and (3) BIM-based building energy analysis : a lack of procedures for reflecting the as-is building condition in the energy modeling process. Beyond the technical contributions, the domain expert surveys conducted in this dissertation show that the proposed methods have potential to improve the quality of thermographic inspection processes and complement the current building energy analysis tools.

Development of Design Guidance for K-12 Schools from 30% to 50% Energy Savings: Preprint

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pless, S.; Torcellini, P.; Long, N.

2008-07-01

This paper describes the development of energy efficiency recommendations for achieving 30% whole-building energy savings in K-12 schools over levels achieved by following the ANSI/ASHRAE/IESNA Standard 90.1. These design recommendations look at building envelope, fenestration, lighting systems (including electrical lights and daylighting), HVAC systems, building automation and controls, outside air treatment, and service water heating.

Monitoring Sealant Durability during Instrumented Outdoor Exposure with Variation in Prestrain

Treesearch

Gregory T. Schueneman; Steven Lacher; Christopher G. Hunt

2015-01-01

Sealants are a vital part of a building’s environmental barrier envelope. Their ability to bond to numerous dissimilar substrates and form cure-in-place seals results in their widespread use throughout single story and high rise buildings. A typical 40 story concrete and glass facade building can have 70 miles of sealant bonds. Failure of sealants can lead to energy...

Technologies developed by CNES balloon team

NASA Astrophysics Data System (ADS)

Sosa-Sesma, Sergio; Charbonnier, Jean-Marc; Deramecourt, Arnaud

CNES balloon team develops and operates all the components of this kind of vehicle: it means envelope and gondola. This abstract will point out only developments done for envelope. Nowadays CNES offers to scientists four types of envelops that cover a large range of mission demands. These envelops are: 1. Zero pressure balloons: Size going from 3,000m3 to 600,000m3, this kind of envelop is ideal for short duration flights (a few hours) but if we use an intelligent management of ballast consumption and if we chose the best launch site, it is possible to perform medium duration flights (10/20 days depending on the ballast on board). Flight train mass starts at 50kg for small balloons and reach 1000kg for larger ones. Zero pressure balloons are inflated with helium gas. 2. Super pressure balloons: Diameter going from 2.5m to 12m, this kind of envelop is ideal for long duration flights (1 to 6 months). Flight train is inside the envelop for small balloons, it means 2.5 diameter meters which is usually called BPCL (Super pressure balloon for Earth boundary layer) and it is about 3kg of mass. Larger ones could lift external flight trains about 50kg of mass. Super pressure balloons are inflated with helium gas. 3. MIR balloons: Size going from 36,000m3 to 46,000m3. Ceiling is reach with helium gas but after three days helium is no longer present inside and lift force is produced by difference of temperature between air inside and air of atmosphere. Flight trains must not be over 50kg. 4. Aero Clipper balloons: A concept to correlate measurements done in oceans and in nearest layers of atmosphere simultaneously. Flight train is made by a "fish" that drags inside water and an atmospheric gondola few meters above "fish", both pushed by a balloon which profits of the wind force. Materials used for construction and assembling depend on balloon type; they are usually made of polyester or polyethylene. Thickness varies from 12 micrometers to 120 micrometers. Balloon assembling is made at ZODIAC site (near Toulouse) by Zodiac teams although all mechanical machines belong to CNES. These machines had been developed by CNES to cut, to weld and to thermo-joint the different parts of the balloon.

Cryo-Electron Tomography of Marburg Virus Particles and Their Morphogenesis within Infected Cells

PubMed Central

Kolesnikova, Larissa; Welsch, Sonja; Krähling, Verena; Davey, Norman; Parsy, Marie-Laure; Becker, Stephan; Briggs, John A. G.

2011-01-01

Several major human pathogens, including the filoviruses, paramyxoviruses, and rhabdoviruses, package their single-stranded RNA genomes within helical nucleocapsids, which bud through the plasma membrane of the infected cell to release enveloped virions. The virions are often heterogeneous in shape, which makes it difficult to study their structure and assembly mechanisms. We have applied cryo-electron tomography and sub-tomogram averaging methods to derive structures of Marburg virus, a highly pathogenic filovirus, both after release and during assembly within infected cells. The data demonstrate the potential of cryo-electron tomography methods to derive detailed structural information for intermediate steps in biological pathways within intact cells. We describe the location and arrangement of the viral proteins within the virion. We show that the N-terminal domain of the nucleoprotein contains the minimal assembly determinants for a helical nucleocapsid with variable number of proteins per turn. Lobes protruding from alternate interfaces between each nucleoprotein are formed by the C-terminal domain of the nucleoprotein, together with viral proteins VP24 and VP35. Each nucleoprotein packages six RNA bases. The nucleocapsid interacts in an unusual, flexible “Velcro-like” manner with the viral matrix protein VP40. Determination of the structures of assembly intermediates showed that the nucleocapsid has a defined orientation during transport and budding. Together the data show striking architectural homology between the nucleocapsid helix of rhabdoviruses and filoviruses, but unexpected, fundamental differences in the mechanisms by which the nucleocapsids are then assembled together with matrix proteins and initiate membrane envelopment to release infectious virions, suggesting that the viruses have evolved different solutions to these conserved assembly steps. PMID:22110401

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘E’, ROOF PLAN, ARCHITECTURAL. Sheet 22 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘D’, 2ND FLOOR PLAN, ARCHITECTURAL. Sheet 38 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘D’, MAIN FLOOR PLAN, ARCHITECTURAL. Sheet 39 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘E’, MAIN FLOOR PLAN, ARCHITECTURAL. Sheet 23 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘D’, ROOF PLAN, ARCHITECTURAL. Sheet 36 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘D’, 3RD FLOOR PLAN, ARCHITECTURAL. Sheet 37 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘B’, MAIN FLOOR PLAN, ARCHITECTURAL. Sheet 30 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘C’, 2ND FLOOR PLAN, ARCHITECTURAL. Sheet 15 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘C’, ROOF PLAN, ARCHITECTURAL. Sheet 14 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘B’, ROOF PLAN, ARCHITECTURAL. Sheet 28 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘C’, MAIN FLOOR PLAN, ARCHITECTURAL. Sheet 16 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘B’, 2ND FLOOR PLAN, ARCHITECTURAL. Sheet 29 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS, HIGH BAY AREA. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS, HIGH BAY AREA. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. TRANSFER AISLE NORTH DOOR,ARCHITECTURAL NORTH ELEVATION AND MISC. DETAILS. Sheet 78 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Russian Apartment Building Thermal Response Models for Retrofit Selection and Verification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Armstrong, Peter R.; Dirks, James A.; Reilly, Raymond W.

2000-08-21

The Enterprise Housing Divestiture Project (EHDP) aims to identify cost-effective energy efficiency and conservation measures for Russian apartment buildings and to implement these measures in the entire stock of buildings undergoing divestiture in six cities. Short-term measurements of infiltration and exterior wall heat-loss coefficient were made in the cities of Cheropovets, Orenburg, Petrozavodsk, Ryazan, and Vladimir. Long-term monitoring equipment was installed in six or more buildings in the aforementioned and in the city of Volxhov. The results of these measurements will be used to calibrate models used to select optimal retrofit packages and to verify energy savings. The retrofit categoriesmore » representing the largest technical potential in these buildings are envelope, heat recovery, and heating/hot water system improvements. This paper describes efforts to establish a useful thermal model calibration process. The model structures and analytical methods for obtaining building parameters from time series weather, energy use, and thermal response data are developed. Our experience applying these methods to two, nominally identical 5-story apartment buildings in the city of Ryazan is presented. Building envelope UA?s inferred from measured whole-building thermal response data are compared with UA?s based on window and wall U-values (the latter obtained by ASTM in-situ measurements of 20 wall sections in various Ryazan panel buildings) as well. The UA's obtained by these completely independent measurements differ by less than 10%.« less

a Risk Based Methodology to Assess the Energy Efficiency Improvements in Traditionally Constructed Buildings

NASA Astrophysics Data System (ADS)

Herrera, D.; Bennadji, A.

2013-07-01

In order to achieve the CO2 reduction targets set by the Scottish government, it will be necessary to improve the energy efficiency of existing buildings. Within the total Scottish building stock, historic and traditionally constructed buildings are an important proportion, in the order of 19 % (Curtis, 2010), and represent cultural, emotional and identity values that should be protected. However, retrofit interventions could be a complex operation because of the several aspects that are involved in the hygrothermal performance of traditional buildings. Moreover, all these factors interact with each other and therefore need to be analysed as a whole. Upgrading the envelope of traditional buildings may produce severe changes to the moisture migration leading to superficial or interstitial condensation and thus fabric decay and mould growth. Retrofit projects carried out in the past have failed because of the misunderstanding, or the lack of expert prediction, of the potential consequences associated to the envelope's alteration. The evaluation of potential risks, prior to any alteration on building's physics in order to improve its energy efficiency, is critical to avoid future damage on the wall's performance or occupants' health and well being. The aim of this PhD research project is to point out the most critical aspects related to the energy efficiency improvement of traditional buildings and to develop a risk based methodology that helps owners and practitioners during the decision making process.

Alteration of Mature Nucleocapsid and Enhancement of Covalently Closed Circular DNA Formation by Hepatitis B Virus Core Mutants Defective in Complete-Virion Formation.

PubMed

Cui, Xiuji; Luckenbaugh, Laurie; Bruss, Volker; Hu, Jianming

2015-10-01

Assembly of hepatitis B virus (HBV) begins with packaging of the pregenomic RNA (pgRNA) into immature nucleocapsids (NC), which are converted to mature NCs containing the genomic relaxed circular (RC) DNA as a result of reverse transcription. Mature NCs have two alternative fates: (i) envelopment by viral envelope proteins, leading to secretion extracellularly as virions, or (ii) disassembly (uncoating) to deliver their RC DNA content into the host cell nucleus for conversion to the covalently closed circular (CCC) DNA, the template for viral transcription. How these two alternative fates are regulated remains to be better understood. The NC shell is composed of multiple copies of a single viral protein, the HBV core (HBc) protein. HBc mutations located on the surface of NC have been identified that allow NC maturation but block its envelopment. The potential effects of some of these mutations on NC uncoating and CCC DNA formation have been analyzed by transfecting HBV replication constructs into hepatoma cells. All envelopment-defective HBc mutations tested were competent for CCC DNA formation, indicating that core functions in envelopment and uncoating/nuclear delivery of RC DNA were genetically separable. Some of the envelopment-defective HBc mutations were found to alter specifically the integrity of mature, but not immature, NCs such that RC DNA became susceptible to nuclease digestion. Furthermore, CCC DNA formation could be enhanced by NC surface mutations that did or did not significantly affect mature NC integrity, indicating that the NC surface residues may be closely involved in NC uncoating and/or nuclear delivery of RC DNA. Hepatitis B virus (HBV) infection is a major health issue worldwide. HBV assembly begins with the packaging into immature nucleocapsids (NCs) of a viral RNA pregenome, which is converted to the DNA genome in mature NCs. Mature NCs are then selected for envelopment and secretion as complete-virion particles or, alternatively, can deliver their DNA to the host cell nucleus to maintain the viral genome as nuclear episomes, which are the basis for virus persistence. Previous studies have identified mutations on the capsid surface that selectively block NC envelopment without affecting NC maturation. We have now discovered that some of the same mutations result in preferential alteration of mature NCs and increased viral nuclear episomes. These findings provide important new insights into the regulation of the two alternative fates of mature NCs and suggest new ways to perturb viral persistence by manipulating levels of viral nuclear episomes. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

ARCHITECTURAL, 777M, PHYSICS ASSEMBLY LABORATORY BUILDING, PLAN OF +13’1” AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

ARCHITECTURAL, 777-M, PHYSICS ASSEMBLY LABORATORY BUILDING, PLAN OF +13’-1” AND +27’-0” FLOOR LEVELS (W157114) - Physics Assembly Laboratory, Area A/M, Savannah River Site, Aiken, Aiken County, SC

Novel dynamic thermal characterization of multifunctional concretes with microencapsulated phase change materials

NASA Astrophysics Data System (ADS)

Pisello, Anna Laura; Fabiani, Claudia; D'Alessandro, Antonella; Cabeza, Luisa F.; Ubertini, Filippo; Cotana, Franco

2017-04-01

Concrete is widely applied in the construction sector for its reliable mechanical performance, its easiness of use and low costs. It also appears promising for enhancing the thermal-energy behavior of buildings thanks to its capability to be doped with multifunctional fillers. In fact, key studies acknowledged the benefits of thermally insulated concretes for applications in ceilings and walls. At the same time, thermal capacity also represents a key property to be optimized, especially for lightweight constructions. In this view, Thermal-Energy Storage (TES) systems have been recently integrated into building envelopes for increasing thermal inertia. More in detail, numerical experimental investigations showed how Phase Change materials (PCMs), as an acknowledged passive TES strategy, can be effectively included in building envelope, with promising results in terms of thermal buffer potentiality. In particular, this work builds upon previous papers aimed at developing the new PCM-filled concretes for structural applications and optimized thermalenergy efficiency, and it is focused on the development of a new experimental method for testing such composite materials in thermal-energy dynamic conditions simulated in laboratory by exposing samples to environmentally controlled microclimate while measuring thermal conductivity and diffusivity by means of transient plane source techniques. The key findings show how the new composites are able to increasingly delay the thermal wave with increasing the PCM concentration and how the thermal conductivity varies during the course of the phase change, in both melting and solidification processes. The new analysis produces useful findings in proposing an effective method for testing composite materials with adaptive thermal performance, much needed by the scientific community willing to study building envelopes dynamics.

Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting

PubMed Central

Müller, Oliver; Ivanova, Lyudmila; Bialy, Dagmara; Pohlmann, Anja; Binz, Anne; Hegemann, Maike; Viejo-Borbolla, Abel; Rosenhahn, Bodo; Bauerfeind, Rudolf; Sodeik, Beate

2017-01-01

Upon reactivation from latency and during lytic infections in neurons, alphaherpesviruses assemble cytosolic capsids, capsids associated with enveloping membranes, and transport vesicles harboring fully enveloped capsids. It is debated whether capsid envelopment of herpes simplex virus (HSV) is completed in the soma prior to axonal targeting or later, and whether the mechanisms are the same in neurons derived from embryos or from adult hosts. We used HSV mutants impaired in capsid envelopment to test whether the inner tegument proteins pUL36 or pUL37 necessary for microtubule-mediated capsid transport were sufficient for axonal capsid targeting in neurons derived from the dorsal root ganglia of adult mice. Such neurons were infected with HSV1-ΔUL20 whose capsids recruited pUL36 and pUL37, with HSV1-ΔUL37 whose capsids associate only with pUL36, or with HSV1-ΔUL36 that assembles capsids lacking both proteins. While capsids of HSV1-ΔUL20 were actively transported along microtubules in epithelial cells and in the somata of neurons, those of HSV1-ΔUL36 and -ΔUL37 could only diffuse in the cytoplasm. Employing a novel image analysis algorithm to quantify capsid targeting to axons, we show that only a few capsids of HSV1-ΔUL20 entered axons, while vesicles transporting gD utilized axonal transport efficiently and independently of pUL36, pUL37, or pUL20. Our data indicate that capsid motility in the somata of neurons mediated by pUL36 and pUL37 does not suffice for targeting capsids to axons, and suggest that capsid envelopment needs to be completed in the soma prior to targeting of herpes simplex virus to the axons, and to spreading from neurons to neighboring cells. PMID:29284065

Inhibitors of SARS-CoV Entry - Identification using an Internally-Controlled Dual Envelope Pseudovirion Assay

PubMed Central

Zhou, Yanchen; Agudelo, Juliet; Lu, Kai; Goetz, David H.; Hansell, Elizabeth; Chen, Yen Ting; Roush, William R.; McKerrow, James; Craik, Charles S.; Amberg, Sean M.; Simmons, Graham

2011-01-01

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged as the causal agent of an endemic atypical pneumonia, infecting thousands of people worldwide. Although a number of promising potential vaccines and therapeutic agents for SARS-CoV have been described, no effective antiviral drug against SARS-CoV is currently available. The intricate, sequential nature of the viral entry process provides multiple valid targets for drug development. Here, we describe a rapid and safe cell-based high-throughput screening system, Dual Envelope Pseudovirion (DEP) Assay, for specifically screening inhibitors of viral entry. The assay system employs a novel dual envelope strategy, using lentiviral pseudovirions as targets whose entry is driven by the SARS-CoV Spike glycoprotein. A second, unrelated viral envelope is used as an internal control to reduce the number of false positives. As an example of the power of this assay a class of inhibitors is reported with the potential to inhibit SARS-CoV at two steps of the replication cycle, viral entry and particle assembly. This assay system can be easily adapted to screen entry inhibitors against other viruses with the careful selection of matching partner virus envelopes. PMID:21820471

7. PHOTOCOPY, PLANS, ELEVATIONS, AND SECTION DRAWING FOR MISSILE TEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. PHOTOCOPY, PLANS, ELEVATIONS, AND SECTION DRAWING FOR MISSILE TEST AND ASSEMBLY BUILDING. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

74. Neg. No. F116A, May 22, 1931, INTERIORPRESSED STEEL BUILDING, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

74. Neg. No. F-116A, May 22, 1931, INTERIOR-PRESSED STEEL BUILDING, SHOWING FENDER CRATE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Bearing defect signature analysis using advanced nonlinear signal analysis in a controlled environment

NASA Technical Reports Server (NTRS)

Zoladz, T.; Earhart, E.; Fiorucci, T.

1995-01-01

Utilizing high-frequency data from a highly instrumented rotor assembly, seeded bearing defect signatures are characterized using both conventional linear approaches, such as power spectral density analysis, and recently developed nonlinear techniques such as bicoherence analysis. Traditional low-frequency (less than 20 kHz) analysis and high-frequency envelope analysis of both accelerometer and acoustic emission data are used to recover characteristic bearing distress information buried deeply in acquired data. The successful coupling of newly developed nonlinear signal analysis with recovered wideband envelope data from accelerometers and acoustic emission sensors is the innovative focus of this research.

Conserved Tryptophan Motifs in the Large Tegument Protein pUL36 Are Required for Efficient Secondary Envelopment of Herpes Simplex Virus Capsids

PubMed Central

Ivanova, Lyudmila; Buch, Anna; Döhner, Katinka; Pohlmann, Anja; Binz, Anne; Prank, Ute; Sandbaumhüter, Malte

2016-01-01

ABSTRACT Herpes simplex virus (HSV) replicates in the skin and mucous membranes, and initiates lytic or latent infections in sensory neurons. Assembly of progeny virions depends on the essential large tegument protein pUL36 of 3,164 amino acid residues that links the capsids to the tegument proteins pUL37 and VP16. Of the 32 tryptophans of HSV-1-pUL36, the tryptophan-acidic motifs 1766WD1767 and 1862WE1863 are conserved in all HSV-1 and HSV-2 isolates. Here, we characterized the role of these motifs in the HSV life cycle since the rare tryptophans often have unique roles in protein function due to their large hydrophobic surface. The infectivity of the mutants HSV-1(17+)Lox-pUL36-WD/AA-WE/AA and HSV-1(17+)Lox-CheVP26-pUL36-WD/AA-WE/AA, in which the capsid has been tagged with the fluorescent protein Cherry, was significantly reduced. Quantitative electron microscopy shows that there were a larger number of cytosolic capsids and fewer enveloped virions compared to their respective parental strains, indicating a severe impairment in secondary capsid envelopment. The capsids of the mutant viruses accumulated in the perinuclear region around the microtubule-organizing center and were not dispersed to the cell periphery but still acquired the inner tegument proteins pUL36 and pUL37. Furthermore, cytoplasmic capsids colocalized with tegument protein VP16 and, to some extent, with tegument protein VP22 but not with the envelope glycoprotein gD. These results indicate that the unique conserved tryptophan-acidic motifs in the central region of pUL36 are required for efficient targeting of progeny capsids to the membranes of secondary capsid envelopment and for efficient virion assembly. IMPORTANCE Herpesvirus infections give rise to severe animal and human diseases, especially in young, immunocompromised, and elderly individuals. The structural hallmark of herpesvirus virions is the tegument, which contains evolutionarily conserved proteins that are essential for several stages of the herpesvirus life cycle. Here we characterized two conserved tryptophan-acidic motifs in the central region of the large tegument protein pUL36 of herpes simplex virus. When we mutated these motifs, secondary envelopment of cytosolic capsids and the production of infectious particles were severely impaired. Our data suggest that pUL36 and its homologs in other herpesviruses, and in particular such tryptophan-acidic motifs, could provide attractive targets for the development of novel drugs to prevent herpesvirus assembly and spread. PMID:27009950

Dynamic Assembly of Brambleberry Mediates Nuclear Envelope Fusion during Early Development

PubMed Central

Abrams, Elliott W.; Zhang, Hong; Marlow, Florence L.; Kapp, Lee; Lu, Sumei; Mullins, Mary C.

2012-01-01

Summary To accommodate the large cells following zygote formation, early blastomeres employ modified cell divisions. Karyomeres are one such modification, a mitotic intermediate wherein individual chromatin masses are surrounded by nuclear envelope, which then fuse to form a single mononucleus. We identified brambleberry, a maternal-effect zebrafish mutant that disrupts karyomere fusion resulting in formation of multiple micronuclei. brambleberry is a previously unannotated gene homologous to Kar5p, which participates in nuclear fusion in yeast. We demonstrate that Brambleberry is required for pronuclear fusion following fertilization in zebrafish. As karyomeres form, Brambleberry localizes to the nuclear envelope with prominent puncta evident near karyomere-karyomere interfaces corresponding to membrane fusion sites. Our studies identify the first factor acting in karyomere fusion and suggest that specialized proteins are necessary for proper nuclear division in large dividing blastomeres. PMID:22863006

Incorporation of homologous and heterologous proteins into the envelope of Moloney murine leukemia virus.

PubMed Central

Suomalainen, M; Garoff, H

1994-01-01

The efficiencies with which homologous and heterologous proteins are incorporated into the envelope of Moloney murine leukemia virus (M-MuLV) have been analyzed by utilizing a heterologous, Semliki Forest virus-driven M-MuLV assembly system and quantitative pulse-chase assays. Homologous M-MuLV spike protein was found to be efficiently incorporated into extracellular virus particles when expressed at a relatively low density at the plasma membrane. In contrast, efficient incorporation of heterologous proteins (the spike complex of Semliki Forest virus and a cytoplasmically truncated mutant of the human transferrin receptor) was observed only when these proteins were expressed at high densities at the cell surface. These results imply that homologous and heterologous proteins are incorporated into the M-MuLV envelope via two distinct pathways. Images PMID:8035486

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS, HIGH BAY AREA. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS, HIGH BAY AREA. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. TRANSFER AISLE NORTH DOOR, ARCHITECTURAL AND STRUCTURAL ELEVATIONS, SECTIONS AND DETAILS. Sheet 79 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Modeling study of seated reach envelopes based on spherical harmonics with consideration of the difficulty ratings.

PubMed

Yu, Xiaozhi; Ren, Jindong; Zhang, Qian; Liu, Qun; Liu, Honghao

2017-04-01

Reach envelopes are very useful for the design and layout of controls. In building reach envelopes, one of the key problems is to represent the reach limits accurately and conveniently. Spherical harmonics are proved to be accurate and convenient method for fitting of the reach capability envelopes. However, extensive study are required on what components of spherical harmonics are needed in fitting the envelope surfaces. For applications in the vehicle industry, an inevitable issue is to construct reach limit surfaces with consideration of the seating positions of the drivers, and it is desirable to use population envelopes rather than individual envelopes. However, it is relatively inconvenient to acquire reach envelopes via a test considering the seating positions of the drivers. In addition, the acquired envelopes are usually unsuitable for use with other vehicle models because they are dependent on the current cab packaging parameters. Therefore, it is of great significance to construct reach envelopes for real vehicle conditions based on individual capability data considering seating positions. Moreover, traditional reach envelopes provide little information regarding the assessment of reach difficulty. The application of reach envelopes will improve design quality by providing difficulty-rating information about reach operations. In this paper, using the laboratory data of seated reach with consideration of the subjective difficulty ratings, the method of modeling reach envelopes is studied based on spherical harmonics. The surface fitting using spherical harmonics is conducted for circumstances both with and without seat adjustments. For use with adjustable seat, the seating position model is introduced to re-locate the test data. The surface fitting is conducted for both population and individual reach envelopes, as well as for boundary envelopes. Comparison of the envelopes of adjustable seat and the SAE J287 control reach envelope shows that the latter is nearly at the middle difficulty level. It is also found that the abilities of reach envelope models in expressing the shape of the reach limits based on spherical harmonics depends both on the terms in the model expression and on the data used to fit the envelope surfaces. Copyright © 2016 Elsevier Ltd. All rights reserved.

Smart Building. Volume 2: System Description

DTIC Science & Technology

2006-05-01

demonstrated.this technology at the 2002 Winter Olympic Games in Salt Lake City, Utah. The system was installed on a building known as Social Hall Plaza...select the detailed engineering contractors. 3.1.3.8 Sealing the Protective Envelope Due to the type of roof construction on the building there was ...in time to support the Olympics . Prototype testing was completed following the Olympics and additional testing may be performed to better

Synthesis of chlorophyll b: Localization of chlorophyllide a oxygenase and discovery of a stable radical in the catalytic subunit

PubMed Central

Eggink, Laura L; LoBrutto, Russell; Brune, Daniel C; Brusslan, Judy; Yamasato, Akihiro; Tanaka, Ayumi; Hoober, J Kenneth

2004-01-01

Background Assembly of stable light-harvesting complexes (LHCs) in the chloroplast of green algae and plants requires synthesis of chlorophyll (Chl) b, a reaction that involves oxygenation of the 7-methyl group of Chl a to a formyl group. This reaction uses molecular oxygen and is catalyzed by chlorophyllide a oxygenase (CAO). The amino acid sequence of CAO predicts mononuclear iron and Rieske iron-sulfur centers in the protein. The mechanism of synthesis of Chl b and localization of this reaction in the chloroplast are essential steps toward understanding LHC assembly. Results Fluorescence of a CAO-GFP fusion protein, transiently expressed in young pea leaves, was found at the periphery of mature chloroplasts and on thylakoid membranes by confocal fluorescence microscopy. However, when membranes from partially degreened cells of Chlamydomonas reinhardtii cw15 were resolved on sucrose gradients, full-length CAO was detected by immunoblot analysis only on the chloroplast envelope inner membrane. The electron paramagnetic resonance spectrum of CAO included a resonance at g = 4.3, assigned to the predicted mononuclear iron center. Instead of a spectrum of the predicted Rieske iron-sulfur center, a nearly symmetrical, approximately 100 Gauss peak-to-trough signal was observed at g = 2.057, with a sensitivity to temperature characteristic of an iron-sulfur center. A remarkably stable radical in the protein was revealed by an isotropic, 9 Gauss peak-to-trough signal at g = 2.0042. Fragmentation of the protein after incorporation of 125I- identified a conserved tyrosine residue (Tyr-422 in Chlamydomonas and Tyr-518 in Arabidopsis) as the radical species. The radical was quenched by chlorophyll a, an indication that it may be involved in the enzymatic reaction. Conclusion CAO was found on the chloroplast envelope and thylakoid membranes in mature chloroplasts but only on the envelope inner membrane in dark-grown C. reinhardtii cells. Such localization provides further support for the envelope membranes as the initial site of Chl b synthesis and assembly of LHCs during chloroplast development. Identification of a tyrosine radical in the protein provides insight into the mechanism of Chl b synthesis. PMID:15086960

DOE Office of Scientific and Technical Information (OSTI.GOV)

Murguia-Berthier, Ariadna; Ramirez-Ruiz, Enrico; Antoni, Andrea

During a common envelope (CE) episode in a binary system, the engulfed companion spirals to tighter orbital separations under the influence of drag from the surrounding envelope material. As this object sweeps through material with a steep radial gradient of density, net angular momentum is introduced into the flow, potentially leading to the formation of an accretion disk. The presence of a disk would have dramatic consequences for the outcome of the interaction because accretion might be accompanied by strong, polar outflows with enough energy to unbind the entire envelope. Without a detailed understanding of the necessary conditions for diskmore » formation during CE, therefore, it is difficult to accurately predict the population of merging compact binaries. This paper examines the conditions for disk formation around objects embedded within CEs using the “wind tunnel” formalism developed by MacLeod et al. We find that the formation of disks is highly dependent on the compressibility of the envelope material. Disks form only in the most compressible of stellar envelope gas, found in envelopes’ outer layers in zones of partial ionization. These zones are largest in low-mass stellar envelopes, but comprise small portions of the envelope mass and radius in all cases. We conclude that disk formation and associated accretion feedback in CE is rare, and if it occurs, transitory. The implication for LIGO black hole binary assembly is that by avoiding strong accretion feedback, CE interactions should still result in the substantial orbital tightening needed to produce merging binaries.« less

Passive solar/earth sheltered office/dormitory cooling season thermal performance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Christian, J.

1984-01-01

Continuous detailed hourly thermal performance measurements have been taken since February 1982 in and around an occupied, underground, 4000 ft/sup 2/ office/dormitory building at the Oak Ridge National Laboratory in Oak Ridge, Tennessee. This building has a number of energy saving features which have been analyzed relative to their performance in a southeastern US climate and with respect to overall commercial building performance. This analysis documents cooling season performance, as well as effects of earth contact, interior thermal mass, an economizer cycle and interface of an efficient building envelope with a central three-ton heat pump. The Joint Institute Dormitory obtainsmore » a cooling energy savings of about 30% compared with an energy-efficient, above-grade structure and has the potential to save as much as 50%. The proper installation of the overhand, interior thermal mass, massive supply duct system, and earth contact team up to prevent summertime overheating. From May through September, this building cost a total of $300 (at 5.7 cents/kWh) to cool and ventilate 24 hours per day. Besides thermal performance of the building envelope, extensive comfort data was taken illustrating that at least 90% of the occupants are comfortable all of the time according to the PMV measurements.« less

41. ARAIII Prototype assembly and evaluation building ARA630. West end ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

41. ARA-III Prototype assembly and evaluation building ARA-630. West end and south side of building. Camera facing northeast. Ineel photo no. 3-22. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID

SOUTHERN END OF ASSEMBLY PLANT, LOOKING N ON SECOND FLOOR, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

SOUTHERN END OF ASSEMBLY PLANT, LOOKING N ON SECOND FLOOR, WHICH DID NOT EXIST ON DURING SHIP BUILDING DAYS. NOTE VEHICLE ELEVATOR. ABOVE AND TO THE LEFT IS SHIP BUILDING BALCONY. - B Building, 3001 Miller Road, Dearborn, MI

56. Neg. No. F161, Mar 18, 1933, INTERIOROFFICE BUILDING, LOBBY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

56. Neg. No. F-161, Mar 18, 1933, INTERIOR-OFFICE BUILDING, LOBBY AND STAIRWELL, SHOWING DAMAGE AFTER AN EARTHQUAKE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

ARCHITECTURE AS PEDAGOGY: INTERDISCIPLINARY DESIGN AND CREATION OF A CARBON NEUTRAL IDAHO ENVIRONMENTAL LEARNING CENTER AT THE UNIVERSITY OF IDAHO MCCALL FIELD CAMPUS

EPA Science Inventory

Output 1. (short-term) Design a carbon neutral field campus with the following design components: structural systems, building envelope, environmental systems, site construction, building materials, information technology, spatial systems and integration ...