Buoyant densities of phototrophic sulfur bacteria and cyanobacteria

NASA Technical Reports Server (NTRS)

Guerrero, R.

1985-01-01

The buoyant densities of bacterial cells are greatly influenced by the accumulation of intracellular reserve material. The buoyant density of phototrophic bacteria that are planktonic is of particular interest, since these organisms must remain in the photic zone of the water column for optimal growth. Separation of cells by their buoyant density may also be of use in separating and identifying organisms from a natural population. The bacteria used were obtained from pure cultures, enrichments, or samples taken directly from the environment.

Observations of currents and density structure across a buoyant plume front

USGS Publications Warehouse

Gelfenbaum, G.; Stumpf, R.P.

1993-01-01

Observations of the Mobile Bay, Alabama, plume during a flood event in April 1991 reveal significant differences in the current field on either side of a front associated with the buoyant plume. During a strong southeasterly wind, turbid, low salinity water from Mobile Bay was pushed through an opening in the west side of the ebb-tidal delta and moved parallel to the coast. A stable front developed between the low salinity water of the buoyant plume (11‰) and the high salinity coastal water (>23‰) that was being forced landward by the prevailing winds. Despite the shallow water depth of 6 m, measurements of currents, temperature, and salinity show large shears and density gradients in both the vertical and the horizontal directions. At a station outside of the buoyant plume, currents at 0.5 m and 1.5 m below the surface were in the same direction as the wind. Inside the plume, however, currents at 0.5 m below the surface were parallel to the coast, 45°, off the direction of the wind and the magnitude was 45% larger than the magnitude of the surface currents outside the plume. Beneath the level of the plume, the currents were identical to the wind-driven currents in the ambient water south of the front. Our observations suggest that the wind-driven surface currents of the ambient water converged with the buoyant plume at the front and were subducted beneath the plume. The motion of the ambient coastal surface water was in the direction of the local wind stress, however, the motion of the plume had no northerly component of motion. The plume also did not show any flow toward the front, suggesting a balance between the northerly component of wind stress and the southerly component of buoyant spreading. In addition, the motion of the plume did not appear to affect the motion of the underlying ambient water, suggesting a lack of mixing between the two waters.

Determination of buoyant density and sensitivity to chloroform and freon for the etiological agent of infectious salmonid anaemia

USGS Publications Warehouse

Christie, K.E.; Hjeltnes, B.; Uglenes , I.; Winton, J.R.

1993-01-01

Plasma was collected from Atlantic salmon Salrno salar with acute infectious salmon anaemia (ISA) and used to challenge Atlantic salmon parr by intraperitoneal injection. Treatment of plasma with the lipid solvent, chloroform, showed that the etiological agent of ISA contained essential lipids, probably as a viral envelope. Some infectivity remained following treatment with freon. Injection challenges using fractions from equilibrium density gradient centrifugation of plasma from fish with acute ISA revealed a band of infectivity in the range 1.184 to 1.262 g cm-3. The band was believed to conta~n both complete ISA-virus particles and infectious particles lacking a complete envelope, nucleocapsid or genome. Density gradient centrifugation of infectious plasma for enrichment of the putative ISA virus appeared to offer a suitable method for obtaining virus-specific nucleic acid for use in the construction of cDNA libraries. 

Sedimentation dynamics and equilibrium profiles in multicomponent mixtures of colloidal particles.

PubMed

Spruijt, E; Biesheuvel, P M

2014-02-19

In this paper we give a general theoretical framework that describes the sedimentation of multicomponent mixtures of particles with sizes ranging from molecules to macroscopic bodies. Both equilibrium sedimentation profiles and the dynamic process of settling, or its converse, creaming, are modeled. Equilibrium profiles are found to be in perfect agreement with experiments. Our model reconciles two apparently contradicting points of view about buoyancy, thereby resolving a long-lived paradox about the correct choice of the buoyant density. On the one hand, the buoyancy force follows necessarily from the suspension density, as it relates to the hydrostatic pressure gradient. On the other hand, sedimentation profiles of colloidal suspensions can be calculated directly using the fluid density as apparent buoyant density in colloidal systems in sedimentation-diffusion equilibrium (SDE) as a result of balancing gravitational and thermodynamic forces. Surprisingly, this balance also holds in multicomponent mixtures. This analysis resolves the ongoing debate of the correct choice of buoyant density (fluid or suspension): both approaches can be used in their own domain. We present calculations of equilibrium sedimentation profiles and dynamic sedimentation that show the consequences of these insights. In bidisperse mixtures of colloids, particles with a lower mass density than the homogeneous suspension will first cream and then settle, whereas particles with a suspension-matched mass density form transient, bimodal particle distributions during sedimentation, which disappear when equilibrium is reached. In all these cases, the centers of the distributions of the particles with the lowest mass density of the two, regardless of their actual mass, will be located in equilibrium above the so-called isopycnic point, a natural consequence of their hard-sphere interactions. We include these interactions using the Boublik-Mansoori-Carnahan-Starling-Leland (BMCSL) equation of state. Finally, we demonstrate that our model is not limited to hard spheres, by extending it to charged spherical particles, and to dumbbells, trimers and short chains of connected beads.

An ultracentrifugation analysis of two hundred fish genomes.

PubMed

Bucciarelli, Giuseppe; Bernardi, Giacomo; Bernardi, Giorgio

2002-08-07

The goal of this study was to provide a comprehensive view of the compositional characteristics of fish genomes. We therefore expanded the number of fish species that we had explored so far in their DNAs by analytical ultracentrifugation in CsCl density gradient from 122 to 201. This study included representatives from three out of nine orders of Elasmobranchs (sharks and rays), both orders of dipnoan lungfishes, and both orders of chondrosteans (sturgeons and bichirs). We also studied 19 out of 38 teleostean orders, which represent all but four (minor) superorders of the subdivision Teleostei, a group comprising about 23,600 species (96% of all extant fishes). This leaves for further studies two subclasses, Holocephali (chimaeras), and Coelacanthimorpha (gombessas). In spite of this substantial increase in the number of species and orders analysed, all average properties (the modal buoyant density, rho(0), the average buoyant density, , the CsCl profile asymmetry, A, and the compositional heterogeneity, H), and all their ranges were unchanged compared to a previous study [J. Mol. Evol. 31 (1990) 265]. This suggests that, in all likelihood, the properties reported in the present paper can be considered as generally valid for all fish genomes.

CONSEQUENCES OF NON-LINEAR DENSITY EFFECTS ON BUOYANCY AND PLUME BEHAVIOR

EPA Science Inventory

Aquatic plumes, as turbulent streams, grow by entraining ambient water. Buoyant plumes rise and dense ones sink, but, non-linear kinetic effects can reverse the buoyant force in mid-phenomenon. The class of nascent-density plumes begin as buoyant, upwardly accelerating plumes tha...

On the rising motion of a drop in stratified fluids

NASA Astrophysics Data System (ADS)

Bayareh, M.; Doostmohammadi, A.; Dabiri, S.; Ardekani, A. M.

2013-10-01

The rising dynamics of a deformable drop in a linearly stratified fluid is numerically obtained using a finite-volume/front-tracking method. Our results show that the drag coefficient of a spherical drop in a stratified fluid enhances as C_{d,s}/C_{d,h}-1˜ Fr_d^{-2.86} for drop Froude numbers in the range of 4 < Frd < 16. The role of the deformability of the drop on the temporal evolution of the motion is investigated along with stratification and inertial effects. We also present the important role of stratification on the transient rising motion of the drop. It is shown that a drop can levitate in the presence of a vertical density gradient. The drop undergoes a fading oscillatory motion around its neutrally buoyant position except for high viscosity ratio drops where the oscillation occurs around a density level lighter than the neutral buoyancy level. In addition, a detailed characterization of the flow signature of a rising drop in a linearly stratified fluid including the buoyancy induced vortices and the resultant buoyant jet is presented.

High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol.

PubMed

Andeer, Peter; Strand, Stuart E; Stahl, David A

2012-01-01

Stable-isotope probing (SIP) has proved a valuable cultivation-independent tool for linking specific microbial populations to selected functions in various natural and engineered systems. However, application of SIP to microbial populations with relatively minor buoyant density increases, such as populations that utilize compounds as a nitrogen source, results in reduced resolution of labeled populations. We therefore developed a tandem quantitative PCR (qPCR)-TRFLP (terminal restriction fragment length polymorphism) protocol that improves resolution of detection by quantifying specific taxonomic groups in gradient fractions. This method combines well-controlled amplification with TRFLP analysis to quantify relative taxon abundance in amplicon pools of FAM-labeled PCR products, using the intercalating dye EvaGreen to monitor amplification. Method accuracy was evaluated using mixtures of cloned 16S rRNA genes, DNA extracted from low- and high-G+C bacterial isolates (Escherichia coli, Rhodococcus, Variovorax, and Microbacterium), and DNA from soil microcosms amended with known amounts of genomic DNA from bacterial isolates. Improved resolution of minor shifts in buoyant density relative to TRFLP analysis alone was confirmed using well-controlled SIP analyses.

Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo.

PubMed

Hayashi, Y; Ando, T; Utagawa, E; Sekine, S; Okada, S; Yabuuchi, K; Miki, T; Ohashi, M

1989-08-01

Small, round-structured virus (SRSV) was detected in a stool specimen of a patient during an acute gastroenteritis outbreak in Tokyo and was tentatively named SRSV-9. SRSV-9 was purified by sucrose velocity gradient centrifugation after CsCl density gradient centrifugation. The buoyant density of SRSV-9 appeared to be 1.36 g/ml in CsCl. A Western blot (immunoblot) assay using the biotin-avidin system revealed that SRSV-9 was antigenically related to the Hawaii agent but distinct from the Norwalk agent and contained a single major structural protein with a molecular size of 63.0 +/- 0.6 kilodaltons. The prevalence of SRSV-9 infection in Tokyo was surveyed by the Western blot antibody assay by using a crude virus preparation as the antigen. Seroconversion was observed in 56.5% of the patients involved in the outbreaks from which SRSV was detected by electron microscopy.

Supercoiled circular DNA of an insect granulosis virus

PubMed Central

Tweeten, Kathleen A.; Bulla, Lee A.; Consigli, Richard A.

1977-01-01

The DNA of the granulosis virus of the Indian meal moth, Plodia interpunctella, was characterized by physical chemical and electron microscopic techniques. Twenty-five percent of the DNA extracted from purified virus was isolated as supercoiled circular molecules. The remaining 75% consisted of relaxed circular molecules. These molecular forms were indicated by the production of two radioactive bands during sedimentation of 3H-labeled granulosis virus DNA in alkaline sucrose gradients or in equilibrium density gradients of neutral cesium chloride/propidium iodide. Electron microscopic visualization of the DNA that banded at the higher density in the latter gradients revealed supercoiled structures whereas that of DNA that banded at the lower density demonstrated relaxed circular molecules. The superhelical molecules were converted to relaxed circles by treatment with pancreatic DNase. The molecular weight of the viral DNA was calculated to be 81 × 106 by sedimentation in neutral sucrose and 78 × 106 by sedimentation in alkaline sucrose. The molecular weight estimated from length measurements in electron micrographs was 76 × 106. The buoyant density of the granulosis virus DNA was 1.703 g/cm3 and that of its insect host DNA was 1.697 g/cm3. Equilibrium sedimentation in cesium chloride and thermal denaturation indicated G + C contents of 44% and 39% for the viral and host DNA, respectively. Images PMID:198791

Supercoiled circular DNA of an insect granulosis virus.

PubMed

Tweeten, K A; Bulla, L A; Consigli, R A

1977-08-01

The DNA of the granulosis virus of the Indian meal moth, Plodia interpunctella, was characterized by physical chemical and electron microscopic techniques. Twenty-five percent of the DNA extracted from purified virus was isolated as supercoiled circular molecules. The remaining 75% consisted of relaxed circular molecules. These molecular forms were indicated by the production of two radioactive bands during sedimentation of (3)H-labeled granulosis virus DNA in alkaline sucrose gradients or in equilibrium density gradients of neutral cesium chloride/propidium iodide. Electron microscopic visualization of the DNA that banded at the higher density in the latter gradients revealed supercoiled structures whereas that of DNA that banded at the lower density demonstrated relaxed circular molecules. The superhelical molecules were converted to relaxed circles by treatment with pancreatic DNase. The molecular weight of the viral DNA was calculated to be 81 x 10(6) by sedimentation in neutral sucrose and 78 x 10(6) by sedimentation in alkaline sucrose. The molecular weight estimated from length measurements in electron micrographs was 76 x 10(6). The buoyant density of the granulosis virus DNA was 1.703 g/cm(3) and that of its insect host DNA was 1.697 g/cm(3). Equilibrium sedimentation in cesium chloride and thermal denaturation indicated G + C contents of 44% and 39% for the viral and host DNA, respectively.

Effect of centrifugal fractionation protocols on quality and recovery rate of equine sperm.

PubMed

Edmond, A J; Brinsko, S P; Love, C C; Blanchard, T L; Teague, S R; Varner, D D

2012-03-15

Centrifugal fractionation of semen is commonly done to improve quality of human semen in assisted-reproduction laboratories, allowing sperm separation based on their isopycnic points. Sperm with morphologic abnormalities are often more buoyant, promoting their retention above defined density media, with structurally normal sperm passing through the media following centrifugation. Three experiments were conducted to evaluate the effects of density-medium type, centrifuge-tube size, sperm number, and density-medium volume (column height) on stallion sperm quality and recovery rate in sperm pellets following centrifugation. In all three experiments, equine semen was initially centrifuged to increase sperm concentration. In Experiment 1, semen was layered over continuous or discontinuous gradients. For Experiment 2, semen was layered over three column heights of continuous gradients in 15- or 50-ml conical-bottom tubes. For Experiment 3, increasing sperm numbers were layered over continuous gradient in 15- or 50-ml conical-bottom tubes. Following centrifugation, sperm pellets were evaluated for sperm morphologic quality, motility, DNA integrity, and recovery rate. Centrifugal fractionation improved (P < 0.05) sperm morphology, motility, and DNA integrity, as compared to controls. The continuous gradient increased (P < 0.05) sperm recovery rate relative to the discontinuous gradient, whereas sperm processed in 15-ml tubes yielded higher velocity and higher recovery rates (P < 0.05 for each) than that processed in 50-ml tubes. Sperm recovery rate was not affected (P > 0.05) by column height of gradient. Increasing sperm number subjected to gradient centrifugation decreased (P < 0.05) sperm recovery rate when 15-ml tubes were used. Copyright © 2012 Elsevier Inc. All rights reserved.

Separation of active and inactive fractions from starved culture of Vibrio parahaemolyticus by density dependent cell sorting.

PubMed

Nayak, Binaya Bhusan; Kamiya, Eriko; Nishino, Tomohiko; Wada, Minoru; Nishimura, Masahiko; Kogure, Kazuhiro

2005-01-01

The co-existence of physiologically different cells in bacterial cultures is a general phenomenon. We have examined the applicability of the density dependent cell sorting (DDCS) method to separate subpopulations from a long-term starvation culture of Vibrio parahaemolyticus. The cells were subjected to Percoll density gradient and separated into 12 fractions of different buoyant densities, followed by measuring the cell numbers, culturability, respiratory activity and leucine incorporation activity. While more than 78% of cells were in lighter fractions, about 95% of culturable cells were present in heavier fractions. The high-density subpopulations also had high proportion of cells capable of forming formazan granules. Although this was accompanied by the cell specific INT-reduction rate, both leucine incorporation rates and INT-reduction rates per cell had a peak at mid-density fraction. The present results indicated that DDCS could be used to separate subpopulations of different physiological conditions.

Flow convergence caused by a salinity minimum in a tidal channel

USGS Publications Warehouse

Warner, John C.; Schoellhamer, David H.; Burau, Jon R.; Schladow, S. Geoffrey

2006-01-01

Residence times of dissolved substances and sedimentation rates in tidal channels are affected by residual (tidally averaged) circulation patterns. One influence on these circulation patterns is the longitudinal density gradient. In most estuaries the longitudinal density gradient typically maintains a constant direction. However, a junction of tidal channels can create a local reversal (change in sign) of the density gradient. This can occur due to a difference in the phase of tidal currents in each channel. In San Francisco Bay, the phasing of the currents at the junction of Mare Island Strait and Carquinez Strait produces a local salinity minimum in Mare Island Strait. At the location of a local salinity minimum the longitudinal density gradient reverses direction. This paper presents four numerical models that were used to investigate the circulation caused by the salinity minimum: (1) A simple one-dimensional (1D) finite difference model demonstrates that a local salinity minimum is advected into Mare Island Strait from the junction with Carquinez Strait during flood tide. (2) A three-dimensional (3D) hydrodynamic finite element model is used to compute the tidally averaged circulation in a channel that contains a salinity minimum (a change in the sign of the longitudinal density gradient) and compares that to a channel that contains a longitudinal density gradient in a constant direction. The tidally averaged circulation produced by the salinity minimum is characterized by converging flow at the bed and diverging flow at the surface, whereas the circulation produced by the constant direction gradient is characterized by converging flow at the bed and downstream surface currents. These velocity fields are used to drive both a particle tracking and a sediment transport model. (3) A particle tracking model demonstrates a 30 percent increase in the residence time of neutrally buoyant particles transported through the salinity minimum, as compared to transport through a constant direction density gradient. (4) A sediment transport model demonstrates increased deposition at the near-bed null point of the salinity minimum, as compared to the constant direction gradient null point. These results are corroborated by historically noted large sedimentation rates and a local maximum of selenium accumulation in clams at the null point in Mare Island Strait.

A novel method to create high density stratification with matching refractive index for optical flow investigations

NASA Astrophysics Data System (ADS)

Krohn, Benedikt; Manera, Annalisa; Petrov, Victor

2018-04-01

Turbulent mixing in stratified environments represents a challenging task in experimental turbulence research, especially when large density gradients are desired. When optical measurement techniques like particle image velocimetry (PIV) are applied to stratified liquids, it is common practice to combine two aqueous solutions with different density but equal refractive index, to suppress particle image deflections. While refractive image matching (RIM) has been developed in the late 1970s, the achieved limit of 4% density ratio was not rivalled up to day. In the present work, we report a methodology, based on the behavior of excess properties and their change in a multicomponent system while mixing, that allows RIM for solutions with higher density differences. The methodology is then successfully demonstrated using a ternary combination of water, isopropanol and glycerol, for which RIM in presence of a density ratio of 8.6% has been achieved. Qualitative PIV results of a turbulent buoyant jet with 8.6% density ratio are shown.

On the mound of Macrotermes michaelseni as an organ of respiratory gas exchange.

PubMed

Turner, J S

2001-01-01

Patterns and rates of air movements in the mounds and nests of Macrotermes michaelseni were studied using tracer methods. Wind is a significant source of energy for powering nest ventilation, despite the mound being a completely enclosed structure. Nests are ventilated by a tidal movement of air driven by temporal variation in wind speed and wind direction. Density gradients sufficiently steep to drive bulk flow by natural convection will be rare. However, metabolism-induced buoyant forces may interact with wind energy in a way that promotes homeostasis of the mound atmosphere.

Surface-Active Agents for Isolation of the Core Component of Avian Myeloblastosis Virus 1

PubMed Central

Stromberg, Kurt

1972-01-01

Sixty-one surface-active agents were evaluated in a procedure designed to assess their ability to remove the envelope from the core component of avian myeloblastosis virus (AMV). The procedure consisted of centrifugation of intact AMV through a series of sucrose gradients each containing an upper layer of agent at one of eight concentrations between 0.01 and 10%. The effectiveness of an agent in producing AMV cores was indicated by (i) the appearance of light-scattering bands in the region of core buoyant density in gradient tubes; (ii) the range of surfactant concentration over which these bands appeared; and (iii) an electron microscopy assessment by the negative-staining technique of the relative proportion of core to non-core material in each of these bands. Six nonionic surfactants were selected by this screening method for comparison in regard to recovery of core protein and endogenous ribonucleic acid (RNA)-dependent deoxyribonucleic acid (DNA) polymerase activity, as well as further morphologic evaluation by electron microscopy. The nonionic surfactants of the polyoxyethylene alcohol class (particularly, Sterox SL) were most effective. Nonionic surfactants of the polyoxyethylene alkylphenol class (particularly, Nonidet P-40) were also effective. Sterox SL and Nonidet P-40 each gave a more than fivefold increase in specific activity of endogenous RNA-dependent DNA polymerase, and each gave a low recovery of core protein. Sterox SL did not interfere to the extent that Nonidet P-40 did in procedures which involved spectrophotometric assay at 260 nm. The use of Sterox SL resulted in the least envelope contamination of core preparations by electron microscopy examination, the most recovery of protein and endogenous RNA-dependent DNA polymerase activity, and a core buoyant density in sucrose of 1.27 g/ml. Images PMID:4112071

The Purification and Concentration of Hog Cholera Virus*

PubMed Central

Cunliffe, H. R.; Rebers, P. A.

1968-01-01

Partial purification of hog cholera virus (HCV) using a simple batch-type chromatographic procedure with magnetic ferric oxide (MFO) is described. Infectious HCV was adsorbed from isotonic solutions to MFO and was eluted under conditions of low ionic strength and high pH. Aqueous solutions of 0.01 M sodium cyanide or 0.0003 M ammonium hydroxide effectively dissociated MFO-HCV complexes. The data indicate that 50 to 100% of the original HCV infectivity was recovered concomitant with a 90 to 95% reduction of extraneous organic nitrogen. MFO-purified HCV was concentrated by density gradient type centrifugations in buffered solutions of cesium chloride and sucrose. Prolonged isodensity centrifugations of concentrated MFO-purified HCV indicated a buoyant density of 1.14 to 1.15 gm/ml for the strain of virus used. PMID:15846899

Multi-gradient drilling method and system

DOEpatents

Maurer, William C.; Medley, Jr., George H.; McDonald, William J.

2003-01-01

A multi-gradient system for drilling a well bore from a surface location into a seabed includes an injector for injecting buoyant substantially incompressible articles into a column of drilling fluid associated with the well bore. Preferably, the substantially incompressible articles comprises hollow substantially spherical bodies.

The effects of Venus' thermal structure on buoyant magma ascent

NASA Technical Reports Server (NTRS)

Sakimoto, S. E. H.; Zuber, M. T.

1992-01-01

The recent Magellan images have revealed a broad spatial distribution of surface volcanism on Venus. Previous work in modeling the ascent of magma on both Venus and Earth has indicated that the planetary thermal structure significantly influences the magmatic cooling rates and thus the amount of magma that can be transported to the surface before solidification. In order to understand which aspects of the thermal structure have the greatest influence on the cooling of ascending magma, we have constructed magma cooling curves for both plutonic and crack buoyant ascent mechanisms, and evaluated the curves for variations in the planetary mantle temperature, thermal gradient curvature with depth, surface temperature gradient, and surface temperature. The planetary thermal structure is modeled as T/T(sub 0) = 1-tau(1-Z/Z(sub 0)(exp n), where T is the temperature, T(sub 0) is the source depth temperature, tau = 1-(T(sub s)/T(sub 0)) where T(sub s) is the planetary surface temperature, Z is the depth, Z(sub 0) is the source depth, and n is a constant that controls thermal gradient curvature with depth. The equation is used both for mathematical convenience and flexibility, as well as its fit to the thermal gradients predicted by the cooling half-space models. We assume a constant velocity buoyant ascent, body-averaged magma temperatures and properties, an initially crystal-free magma, and the same liquidus and solidus for both Venus and Earth.

Isolation and purification of a granulosis virus from infected larvae of the Indian meal moth, Plodia interpunctella.

PubMed

Tweeten, K A; Bulla, L A; Consigli, R A

1977-09-01

A procedure was developed for purification of a granulosis virus inclusion body produced in vivo in the Indian meal moth, Plodia interpunctella (Hübner). Purification was accomplished by differential centrifugation, treatment with sodium deoxycholate, and velocity sedimentation in sucrose gradients. The adequacy of the procedure was confirmed by mixing experiments in which uninfected, radioactively labeled larvae were mixed with infected, unlabeled larvae. After purification, the virus was shown to be free of host tissue, to retain its physical integrity, and to be highly infectious per os. Preparations of purified virus consisted of homogeneous populations of intact inclusion bodies (210 by 380 nm) whose buoyant density was 1.271 g/cm3 when centrifuged to equilibrium in sucrose gradients. Electron microscopy of thin-sectioned virus or of virus sequentially disrupted on electron microscope grids demonstrated three components: protein matrix, envelope, and nucleocapsid.

Isolation and purification of a granulosis virus from infected larvae of the Indian meal moth, Plodia interpunctella.

PubMed Central

Tweeten, K A; Bulla, L A; Consigli, R A

1977-01-01

A procedure was developed for purification of a granulosis virus inclusion body produced in vivo in the Indian meal moth, Plodia interpunctella (Hübner). Purification was accomplished by differential centrifugation, treatment with sodium deoxycholate, and velocity sedimentation in sucrose gradients. The adequacy of the procedure was confirmed by mixing experiments in which uninfected, radioactively labeled larvae were mixed with infected, unlabeled larvae. After purification, the virus was shown to be free of host tissue, to retain its physical integrity, and to be highly infectious per os. Preparations of purified virus consisted of homogeneous populations of intact inclusion bodies (210 by 380 nm) whose buoyant density was 1.271 g/cm3 when centrifuged to equilibrium in sucrose gradients. Electron microscopy of thin-sectioned virus or of virus sequentially disrupted on electron microscope grids demonstrated three components: protein matrix, envelope, and nucleocapsid. Images PMID:334076

Horizontal deflection of single particle in a paramagnetic fluid.

PubMed

Liu, S; Yi, Xiang; Leaper, M; Miles, N J

2014-06-01

This paper describes the horizontal deflection behaviour of a single particle in paramagnetic fluids under a high-gradient superconducting magnetic field. A glass box was designed to carry out experiments and test assumptions. It was found that the particles were deflected away from the magnet bore centre and particles with different density and/or susceptibility settled at a certain position on the container floor due to the combined forces of gravity and magneto-Archimedes as well as lateral buoyant (displacement) force. Matlab was chosen to simulate the movement of the particle in the magnetic fluid, the simulation results were in good accordance with experimental data. The results presented here, though, are still very much in their infancy, which could potentially form the basis of a new approach to separating materials based on a combination of density and susceptibility.

On the Alignment of Strain, Vorticity and Scalar Gradient in Turbulent, Buoyant, Nonpremixed Flames

NASA Technical Reports Server (NTRS)

Boratav, O. N.; Elghobashi, S. E.; Zhong, R.

1999-01-01

The alignment of vorticity and scalar gradient with the eigendirections of the rate of strain tensor is investigated in turbulent buoyant nonpremixed horizontal and vertical flames. The uniqueness of a buoyant nonpremixed flame is that it contains regions with distinct alignment characteristics. The strain-enstrophy angle Psi is used to identify these regions. Examination of the vorticity field and the vorticity production in these different regions indicates that Psi and consequently the alignment properties near the flame surface identified by the mixture fraction band F approximately equals F(sub st) differ from those in the fuel region, F > F(sub st) and the oxidizer region, F < F(sub st). The F approximately equals F(sub st) band shows strain-dominance resulting in vorticity/alpha alignment while F > F(sub st) (and F < F(sub st) for the vertical flame) band(s) show(s) vorticity/beta alignment. The implication of this result is that the scalar dissipation, epsilon(sub F), attains its maximum value always near F approximately equals F(sub st). These results are also discussed within the framework of recent dynamical results [Galanti et al., Nonlinearity 10, 1675 (1997)] suggesting that the Navier-Stokes equations evolved towards an attracting solution. It is shown that the properties of such an attracting solution are also consistent with our results of buoyant turbulent nonpremixed flames.

Analysis of the intermediate size proteoglycans from the developing chick limb buds.

PubMed

Vasan, N

1982-08-01

Limb-bud proteoglycans are heterogeneous molecules which vary in their chemical and physical properties with development. This report describes proteoglycan intermediates (PG-I) that predominate in stage-34 limbs, and compares them with proteoglycan aggregates (PG-A) in stage-38 limbs. We analysed proteoglycans and their components extracted with guanidinium chloride by subjecting them to density gradient centrifugation, molecular sieve chromatography, electrophoretic separation, and selective enzymatic degradation. PG-I and PG-A have similar chondroitin sulphate composition, amino sugars, chondroitin sulphate side-chain length, glycoprotein link factors, and hyaluronic acid binding capacity, and both cross react with antisera prepared against cartilage-specific chick sternal proteoglycans. However, PG-I has lower molecular weight, lower buoyant density, and fewer chondroitin sulphate side chains on the protein core. The PG-I in the developing limb can be considered a mixture of smaller aggregates and cartilage-specific large monomers in which the former predominate.

The influence of buoyant forces and volume fraction of particles on the particle pushing/entrapment transition during directional solidification of Al/SiC and Al/graphite composites

NASA Technical Reports Server (NTRS)

Stefanescu, Doru M.; Moitra, Avijit; Kacar, A. Sedat; Dhindaw, Brij K.

1990-01-01

Directional solidification experiments in a Bridgman-type furnace were used to study particle behavior at the liquid/solid interface in aluminum metal matrix composites. Graphite or silicon-carbide particles were first dispersed in aluminum-base alloys via a mechanically stirred vortex. Then, 100-mm-diameter and 120-mm-long samples were cast in steel dies and used for directional solidification. The processing variables controlled were the direction and velocity of solidification and the temperature gradient at the interface. The material variables monitored were the interface energy, the liquid/particle density difference, the particle/liquid thermal conductivity ratio, and the volume fraction of particles. These properties were changed by selecting combinations of particles (graphite or silicon carbide) and alloys (Al-Cu, Al-Mg, Al-Ni). A model which consideres process thermodynamics, process kinetics (including the role of buoyant forces), and thermophysical properties was developed. Based on solidification direction and velocity, and on materials properties, four types of behavior were predicted. Sessile drop experiments were also used to determine some of the interface energies required in calculation with the proposed model. Experimental results compared favorably with model predictions.

The influence of buoyant forces and volume fraction of particles on the particle pushing/entrapment transition during directional solidification of Al/SiC and Al/graphite composites

NASA Astrophysics Data System (ADS)

Stefanescu, Doru M.; Moitra, Avijit; Kacar, A. Sedat; Dhindaw, Brij K.

1990-01-01

Directional solidification experiments in a Bridgman-type furnace were used to study particle behavior at the liquid/solid interface in aluminum metal matrix composites. Graphite or siliconcarbide particles were first dispersed in aluminum-base alloys via a mechanically stirred vortex. Then, 100-mm-diameter and 120-mm-long samples were cast in steel dies and used for directional solidification. The processing variables controlled were the direction and velocity of solidification and the temperature gradient at the interface. The material variables monitored were the interface energy, the liquid/particle density difference, the particle/liquid thermal conductivity ratio, and the volume fraction of particles. These properties were changed by selecting combinations of particles (graphite or silicon carbide) and alloys (Al-Cu, Al-Mg, Al-Ni). A model which considers process thermodynamics, process kinetics (including the role of buoyant forces), and thermophysical properties was developed. Based on solidification direction and velocity, and on materials properties, four types of behavior were predicted. Sessile drop experiments were also used to determine some of the interface energies required in calculation with the proposed model. Experimental results compared favorably with model predictions.

Physio-chemical and morphological characteristics of avian encephalomyelitis virus

USGS Publications Warehouse

Gosting, L.H.; Grinnell, B.W.; Matsumoto, M.

1980-01-01

Avian encephalomyelitis virus (AEV) was purified from infected chick embryos by a gradient centrifugation in cesium chloride. The virus had a buoyant density of 1.31 to 1.32 g/ml and a sedimentation coefficient of 148 S. The purified AEV was resistant to treatments with chloroform, acid pH or trypsin. The presence of Mg++ stabilized the virus against heat inactivation (56°C, 1 h). Electron microscopic study showed the virus to be 24 to 32 nm in diameter. The surface structure of the purified virus was not easily discernable. Nevertheless, with uranyl acetate-stained particles, Markham's rotation technique revealed that AEV has five-fold symmetry with 32 or 42 capsomers. Exact classification of AEV awaits characterization of the viral nucleic acid.

Sulfur cycling and metabolism of phototrophic and filamentous sulfur bacteria

NASA Technical Reports Server (NTRS)

Guerrero, R.; Brune, D.; Poplawski, R.; Schmidt, T. M.

1985-01-01

Phototrophic sulfur bacteria taken from different habitate (Alum Rock State Park, Palo Alto salt marsh, and Big Soda Lake) were grown on selective media, characterized by morphological and pigment analysis, and compared with bacteria maintained in pure culture. A study was made of the anaerobic reduction of intracellular sulfur globules by a phototrophic sulfur bacterium (Chromatium vinosum) and a filamentous aerobic sulfur bacterium (Beggiatoa alba). Buoyant densities of different bacteria were measured in Percoll gradients. This method was also used to separate different chlorobia in mixed cultures and to assess the relative homogeneity of cultures taken directly or enriched from natural samples (including the purple bacterial layer found at a depth of 20 meters at Big Soda Lake.) Interactions between sulfide oxidizing bacteria were studied.

Acoustic waves in gases with strong pressure gradients

NASA Technical Reports Server (NTRS)

Zorumski, William E.

1989-01-01

The effect of strong pressure gradients on the acoustic modes (standing waves) of a rectangular cavity is investigated analytically. When the cavity response is represented by a sum of modes, each mode is found to have two resonant frequencies. The lower frequency is near the Viaesaela-Brundt frequency, which characterizes the buoyant effect, and the higher frequency is above the ordinary acoustic resonance frequency. This finding shows that the propagation velocity of the acoustic waves is increased due to the pressure gradient effect.

Laboratory flow experiments for visualizing carbon dioxide-induced, density-driven brine convection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kneafsey, T.; Pruess, K.

2009-09-01

Injection of carbon dioxide (CO{sub 2}) into saline aquifers confined by low-permeability cap rock will result in a layer of CO{sub 2} overlying the brine. Dissolution of CO{sub 2} into the brine increases the brine density, resulting in an unstable situation in which more-dense brine overlies less-dense brine. This gravitational instability could give rise to density-driven convection of the fluid, which is a favorable process of practical interest for CO{sub 2} storage security because it accelerates the transfer of buoyant CO{sub 2} into the aqueous phase, where it is no longer subject to an upward buoyant drive. Laboratory flow visualizationmore » tests in transparent Hele-Shaw cells have been performed to elucidate the processes and rates of this CO{sub 2} solute-driven convection (CSC). Upon introduction of CO{sub 2} into the system, a layer of CO{sub 2}-laden brine forms at the CO{sub 2}-water interface. Subsequently, small convective fingers form, which coalesce, broaden, and penetrate into the test cell. Images and time-series data of finger lengths and wavelengths are presented. Observed CO{sub 2} uptake of the convection system indicates that the CO{sub 2} dissolution rate is approximately constant for each test and is far greater than expected for a diffusion-only scenario. Numerical simulations of our system show good agreement with the experiments for onset time of convection and advancement of convective fingers. There are differences as well, the most prominent being the absence of cell-scale convection in the numerical simulations. This cell-scale convection observed in the experiments is probably initiated by a small temperature gradient induced by the cell illumination.« less

Estimating the neutrally buoyant energy density of a Rankine-cycle/fuel-cell underwater propulsion system

NASA Astrophysics Data System (ADS)

Waters, Daniel F.; Cadou, Christopher P.

2014-02-01

A unique requirement of underwater vehicles' power/energy systems is that they remain neutrally buoyant over the course of a mission. Previous work published in the Journal of Power Sources reported gross as opposed to neutrally-buoyant energy densities of an integrated solid oxide fuel cell/Rankine-cycle based power system based on the exothermic reaction of aluminum with seawater. This paper corrects this shortcoming by presenting a model for estimating system mass and using it to update the key findings of the original paper in the context of the neutral buoyancy requirement. It also presents an expanded sensitivity analysis to illustrate the influence of various design and modeling assumptions. While energy density is very sensitive to turbine efficiency (sensitivity coefficient in excess of 0.60), it is relatively insensitive to all other major design parameters (sensitivity coefficients < 0.15) like compressor efficiency, inlet water temperature, scaling methodology, etc. The neutral buoyancy requirement introduces a significant (∼15%) energy density penalty but overall the system still appears to offer factors of five to eight improvements in energy density (i.e., vehicle range/endurance) over present battery-based technologies.

Infectivity and RNA patterns as functions of high- and low-dilution passage of murine sarcoma-leukemia virus: evidence for autointerference within an oncornavirus population.

PubMed

Bondurant, M C; Hackett, A J; Schaffer, F L

1973-05-01

Heterogeneity of buoyant density and RNA content of virions of Moloney murine leukemia-sarcoma complex [MSV (MLV)] was the result of passage at low dilution. Heterogeneous stocks revealed two major RNA components in the population, with the smaller component, apparent mol wt 4 x 10(6) to 5 x 10(6), becoming predominant upon serial passage at low dilution. Concomitantly, infectivity titers of both MLV and MSV decreased upon serial passage at low dilution. MSV (MLV) passaged at high dilution retained high titers and a rather homogeneous high-molecular-weight RNA population characteristic of high-buoyant-density virions. Interference of both MLV and MSV replication was demonstrated by employing mixed inocula containing both low- and high-dilution passage stocks of MSV (MLV). In contrast to results with MSV (MLV), MLV freed of MSV by limit dilution did not show heterogeneity of buoyant density or of RNA when propagated at low dilution.

Infectivity and RNA Patterns as Functions of High- and Low-Dilution Passage of Murine Sarcoma-Leukemia Virus: Evidence for Autointerference Within an Oncornavirus Population

PubMed Central

Bondurant, M. C.; Hackett, A. J.; Schaffer, F. L.

1973-01-01

Heterogeneity of buoyant density and RNA content of virions of Moloney murine leukemia-sarcoma complex [MSV (MLV)] was the result of passage at low dilution. Heterogeneous stocks revealed two major RNA components in the population, with the smaller component, apparent mol wt 4 × 106 to 5 × 106, becoming predominant upon serial passage at low dilution. Concomitantly, infectivity titers of both MLV and MSV decreased upon serial passage at low dilution. MSV (MLV) passaged at high dilution retained high titers and a rather homogeneous high-molecular-weight RNA population characteristic of high-buoyant-density virions. Interference of both MLV and MSV replication was demonstrated by employing mixed inocula containing both low- and high-dilution passage stocks of MSV (MLV). In contrast to results with MSV (MLV), MLV freed of MSV by limit dilution did not show heterogeneity of buoyant density or of RNA when propagated at low dilution. PMID:4350709

3D Numerical Simulation of Turbulent Buoyant Flow and Heat Transport in a Curved Open Channel

USDA-ARS?s Scientific Manuscript database

A three-dimensional buoyancy-extended version of kappa-epsilon turbulence model was developed for simulating the turbulent flow and heat transport in a curved open channel. The density- induced buoyant force was included in the model, and the influence of temperature stratification on flow field was...

Driving bubbles out of glass

NASA Technical Reports Server (NTRS)

Mattox, D. M.

1981-01-01

Surface tension gradient in melt forces gas bubbles to surface, increasing glass strength and transparency. Conventional chemical and buoyant fining are extremely slow in viscous glasses, but tension gradient method moves 250 um bubbles as rapidly as 30 um/s. Heat required for high temperature part of melt is furnished by stationary electrical or natural-gas heater; induction and laser heating are also possible. Method has many applications in industry processes.

Observations of Inner Shelf Flows Influenced by a Small-Scale River Plume in the Northern Gulf of Mexico

NASA Astrophysics Data System (ADS)

Roth, M.; MacMahan, J.; Reniers, A.; Ozgokmen, T. M.

2016-02-01

Recent work has demonstrated that wind and waves are important forcing mechanisms for the inner shelf vertical current structure. Here, the inner shelf flows are evaluated away from an adjacent inlet where a small-scale buoyant plume emerges. The plume's nearshore extent, speed, vertical thickness, and density are controlled by the passage of low-pressure extratropical cyclones that are common in the northern Gulf of Mexico. The colder, brackish plume water provides vertical stratification and a cross-shore density gradient with the warmer, saline oceanic water. An Acoustic Doppler Current Profiler (ADCP) was deployed in 10m water depth as part of an intensive 2-week experiment (SCOPE), which also obtained wind and cross-shelf temperature, salinity, and velocity. The 10m ADCP remained collecting an additional year of velocity observations. The plume was not always present, but episodically influenced the experiment site. When the plume reached the site, the alongshore surface and subsurface typically flowed in opposite directions, likely caused by plume-induced pressure gradients. Plumes that extended into the subsurface appear to have caused depth-averaged onshore flow above that expected from wind and wave-driven forcing. Observations from SCOPE and the 1-year ADCP are used to describe seasonal full-depth flow patterns influenced by wind, waves, and plume presence.

Anaerobic reduction of elemental sulfur by Chromatium vinosum and Beggiatoa alba

NASA Technical Reports Server (NTRS)

Schmidt, T. M.

1985-01-01

The effect of sulfur globules on the buoyant density of Chromatium vinosum and Beggiatoa alba was examined. The potential use of sulfur as a terminal electron acceptor in the anaerobic metabolism of Beggiatoa alba is also examined. The effect of the reduction of intracellular sulfur was investigated during dark metabolism on the buoyant density of C. vinosum. It is hypothesized from the results that the sulfur reduction to sulfide is part of an anaerobic energy operating system. Carbon stored as PHB can be oxidized with the concomitant reduction of sulfur to sulfide.

Free convection in the Matian atmosphere

NASA Technical Reports Server (NTRS)

Clow, G. D.; Haberle, R. M.

1990-01-01

The 'free convective' regime for the Martian atmospheric boundary layer (ABL) was investigated. This state occurs when the mean windspeed at the top of the ABL drops below some critical value U(sub c) and positive buoyant forces are present. Such forces can arise either from vertical temperature or water vapor gradients across the atmospheric surface layer. During free convection, buoyant forces drive narrow plumes that ascend to the inversion height with a return circulation consisting of broad slower-moving downdraughts. Horizontal pressure, temperature, windspeed, and water vapor fluctuations resulting form this circulation pattern can be quite large adjacent to the ground (within the surface layer). The local turbulent fluctuations cause non-zero mean surface stresses, sensible heat fluxes, and latent heat fluxes, even when the mean regional windspeed is zero. Although motions above the surface layer are insensitive to the nature of the surface, the sensible and latent heat fluxes are primarily controlled by processes within the interfacial sublayer immediately adjacent to the ground during free convection. Thus the distinction between aerodynamically smooth and rough airflow within the interfacial sublayer is more important than for the more typical situation where the mean regional windspeed is greater than U(sub c). Buoyant forces associated with water vapor gradients are particularly large on Mars at low pressures and high temperatures when the surface relative humidity is 100 percent, enhancing the likelihood of free convection under these conditions. On this basis, Ingersol postulated the evaporative heat losses from an icy surface on Mars at 237 K and current pressures would exceed the available net radiative flux at the surface, thus prohibiting ice from melting at low atmospheric pressures. Schumann has developed equations describing the horizontal fluctuations and mean vertical gradients occurring during free convection. Schumann's model was generalized to include convection driven by water vapor gradients and to include the effects of circulation above both aerodynamically smooth and rough surfaces.

Design and evaluation of hydrophobic coated buoyant core as floating drug delivery system for sustained release of cisapride

PubMed Central

Jacob, Shery; Nair, Anroop B; Patil, Pandurang N

2010-01-01

An inert hydrophobic buoyant coated–core was developed as floating drug delivery system (FDDS) for sustained release of cisapride using direct compression technology. Core contained low density, porous ethyl cellulose, which was coated with an impermeable, insoluble hydrophobic coating polymer such as rosin. It was further seal coated with low viscosity hydroxypropyl methyl cellulose (HPMC E15) to minimize moisture permeation and better adhesion with an outer drug layer. It was found that stable buoyant core was sufficient to float the tablet more than 8 h without the aid of sodium bicarbonate and citric acid. Sustained release of cisapride was achieved with HPMC K4M in the outer drug layer. The floating lag time required for these novel FDDS was found to be zero, however it is likely that the porosity or density of the core is critical for floatability of these tablets. The in vitro release pattern of these tablets in simulated gastric fluid showed the constant and controlled release for prolonged time. It can be concluded that the hydrophobic coated buoyant core could be used as FDDS for gastroretentive delivery system of cisapride or other suitable drugs. PMID:24825997

Relativistic g-modes in rapidly rotating neutron stars

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gaertig, Erich; Kokkotas, Kostas D.; Department of Physics, Aristotle University of Thessaloniki, Thessaloniki 54124

2009-09-15

We study the g-modes of fast rotating stratified neutron stars in the general relativistic Cowling approximation, where we neglect metric perturbations and where the background models take into account the buoyant force due to composition gradients. This is the first paper studying this problem in a general relativistic framework. In a recent paper [A. Passamonti, B. Haskell, N. Andersson, D. I. Jones, and I. Hawke, Mon. Not. R. Astron. Soc. 394, 730 (2009)], a similar study was performed within the Newtonian framework, where the authors presented results about the onset of CFS-unstable g-modes and the close connection between inertial andmore » gravity modes for sufficiently high rotation rates and small composition gradients. This correlation arises from the interplay between the buoyant force which is the restoring force for g-modes and the Coriolis force which is responsible for the existence of inertial modes. In our relativistic treatment of the problem, we find an excellent qualitative agreement with respect to the Newtonian results.« less

Characterization of the Leptospiral Outer Membrane and Description of Three Novel Leptospiral Membrane Proteins

PubMed Central

Haake, David A.; Matsunaga, James

2002-01-01

The outer membrane (OM) of the mammalian pathogen Leptospira kirschneri was isolated in the form of membrane vesicles by alkaline plasmolysis and separated from the protoplasmic cylinder by sucrose density gradient ultracentrifugation. All four components of the alkaline plasmolysis buffer, including 1.0 M NaCl, 27% sucrose (wt/vol), 2 mM EDTA, and 10 mM Tris (pH 9), were required for efficient OM release, as judged by recovery of leptospiral lipopolysaccharide. Two populations of OM vesicles (OMVs) were recovered, with peak concentrations found in the sucrose gradient at densities of 1.16 and 1.18 g/ml. Transmission electron microscopy revealed that the more buoyant OMV population was smaller (<0.1 μm in diameter) than the denser OMV population (0.2 to 0.3 μm in diameter). The densities of both populations of OMVs were distinct from that of the protoplasmic-cylinder material, which was found in the sucrose gradient at a density of 1.20 g/ml. The OMV fractions were free of protoplasmic-cylinder material, as judged by immunoblotting with antibodies to the endoflagellar sheath protein, heat shock protein GroEL, and two novel cytoplasmic membrane proteins, lipoprotein LipL31 and transmembrane protein ImpL63. The protein components of the OMVs were characterized by one- and two-dimensional immunoblotting and found to include previously described OM proteins (OMPs), including the porin OmpL1; the lipoproteins LipL32, LipL36, and LipL41; and the peripheral membrane protein P31LipL45. A number of less well-characterized OMPs were also identified, including those with molecular masses of 16, 21, 21.5, 22, 31, 36, 44, 48, 90, and 116 kDa. The 48-kDa OMP was identified as a novel OM lipoprotein designated LipL48. The use of membrane-specific markers in OM isolation techniques facilitates an accurate description of the leptospiral OM and its components. PMID:12183539

Fractured-rock hydrogeophysics with electrically conductive and neutrally buoyant tracers

NASA Astrophysics Data System (ADS)

Shakas, A.; Linde, N.; Baron, L.; Le Borgne, T.; Bour, O.; Lavenant, N.; Gerard, M. F.

2016-12-01

Artificial tracer tests help to characterize and understand the dynamics of groundwater systems. This remains a challenging task, especially when dealing with highly heterogeneous formations in which flow can be very localized and the interpretation of tracer breakthrough curves may be ambiguous. As a complement to tracer tests, ground-penetrating radar (GPR) and electrical resistivity tomography can map the space-time migration of electrically conductive tracers. In hydrogeophysics, the most common tracer is dissolved table salt in water. However, conventional salt tracers lead to density effects that are often ignored. Even less than 1% density variations can have a dramatic effect on transport behavior and affect tracer tests in complex ways. Such effects have been demonstrated in our previous experiments that used single-hole GPR to monitor saline push-pull tests in fractured granite. It is possible to model density effects, but this leads to computational complexity and field dynamics that are not necessarily representative of the natural responses of the system. To minimize density effects, we performed a new set of push-pull tests using a neutrally buoyant and electrically conductive tracer at the same test site located close to Ploemeur, France. This novel tracer consists of a mixture of salt (NaCl), water and pure ethanol. Ethanol has a density of 789 g/L at 20° C and is used to counter-act the salt-induced density increase. Our GPR time-lapse images and tracer breakthrough data indicate a largely reversible transport process that confirms the neutral buoyancy of the tracer. Ethanol is biodegradable and does not pose significant environmental issues. Furthermore, calibration of the neutral-buoyant mixture is straightforward to perform in the field using Archimedes principle. Based on these results, we argue that neutrally buoyant ethanol-salt-water mixtures are ideal for a wide variety of hydrogeophysical tracer tests in porous or fractured media.

Turbulence Statistics of a Buoyant Jet in a Stratified Environment

NASA Astrophysics Data System (ADS)

McCleney, Amy Brooke

Using non-intrusive optical diagnostics, turbulence statistics for a round, incompressible, buoyant, and vertical jet discharging freely into a stably linear stratified environment is studied and compared to a reference case of a neutrally buoyant jet in a uniform environment. This is part of a validation campaign for computational fluid dynamics (CFD). Buoyancy forces are known to significantly affect the jet evolution in a stratified environment. Despite their ubiquity in numerous natural and man-made flows, available data in these jets are limited, which constrain our understanding of the underlying physical processes. In particular, there is a dearth of velocity field data, which makes it challenging to validate numerical codes, currently used for modeling these important flows. Herein, jet near- and far-field behaviors are obtained with a combination of planar laser induced fluorescence (PLIF) and multi-scale time-resolved particle image velocimetry (TR-PIV) for Reynolds number up to 20,000. Deploying non-intrusive optical diagnostics in a variable density environment is challenging in liquids. The refractive index is strongly affected by the density, which introduces optical aberrations and occlusions that prevent the resolution of the flow. One solution consists of using index matched fluids with different densities. Here a pair of water solutions - isopropanol and NaCl - are identified that satisfy these requirements. In fact, they provide a density difference up to 5%, which is the largest reported for such fluid pairs. Additionally, by design, the kinematic viscosities of the solutions are identical. This greatly simplifies the analysis and subsequent simulations of the data. The spectral and temperature dependence of the solutions are fully characterized. In the near-field, shear layer roll-up is analyzed and characterized as a function of initial velocity profile. In the far-field, turbulence statistics are reported for two different scales, one capturing the entire jet at near Taylor microscale resolution, and the other, thanks to the careful refractive index matching of the liquids, resolving the Taylor scale at near Kolmogorov scale resolution. This is accomplished using a combination of TR-PIV and long-distance micro-PIV. The turbulence statistics obtained at various downstream locations and magnifications are obtained for density differences of 0%, 1%, and 3%. To validate the experimental methodology and provide a reference case for validation, the effect of initial velocity profile on the neutrally buoyant jet in the self-preserving regime is studied at two Reynolds numbers of 10,000 and 20,000. For the neutrally buoyant jet, it is found that independent of initial conditions the jet follows a self-similar behavior in the far-field; however, the spreading rate is strongly dependent on initial velocity profile. High magnification analysis at the small turbulent length scales shows a flow field where the mean statistics compare well to the larger field of view case. Investigation of the near-field shows the jet is strongly influenced by buoyancy, where an increase in vortex ring formation frequency and number of pairings occur. The buoyant jet with a 1% density difference shows an alteration of the centerline velocity decay, but the radial distribution of the mean axial velocity collapses well at all measurement locations. Jet formation dramatically changes for a buoyant jet with a 3% density difference, where the jet reaches a terminal height and spreads out horizontally at its neutral buoyancy location. Analysis of both the mean axial velocity and strain rates show the jet is no longer self-similar; for example, the mean centerline velocity does not decay uniformly as the jet develops. The centerline strain rates at this density difference also show trends which are strongly influenced by the altered centerline velocity. The overall centerline analysis shows that turbulence suppression occurs as a result of the stratification for both the 1% and 3% density difference. Analysis on the kinetic energy budget shows that the mean convection, production, transportation, and dissipation of energy is altered from stratification. High resolution data of the jet enable flow structures to be captured in the neutrally buoyant region of the flow. Vortices of different sizes are identified. Longer data sets are necessary to perform a statistical analysis of their distribution and to compare them to homogeneous environment case. This multi-scale analysis shows potential for studying energy transfer between length scales.

Large Eddy Simulation of a Forced Round Turbulent Buoyant Plume in Neutral Surroundings

NASA Technical Reports Server (NTRS)

Basu, A. J.; Mansour, N. N.; Koga, Dennis (Technical Monitor)

1999-01-01

Buoyant flows play an important role in various technological and environmental issues. For example, dispersal of pollutants, smoke, or volcano exhaust in the atmosphere, vertical motion of air, formation of clouds and other weather systems, and flows in cooling towers and fires are all determined primarily by buoyancy effects. The buoyancy force in such flows can originate from either a heat source or due to different densities between a fluid and its surroundings. Whatever the cause, the flow can be understood by studying the effects of the tight coupling between the thermal and the velocity fields since density differences can be characterized as temperature differences.

Starting buoyant plumes and vortex ring pinch-off

NASA Astrophysics Data System (ADS)

Pottebaum, Tait; Gharib, Mory

2003-11-01

The vortex ring formation process of a starting buoyant plume was studied experimentally. Buoyant plumes were produced using a heating element at the base of a water tank. The velocity and temperature fields in the flow were measured using digital particle image thermometry and velocimetry (DPITV), allowing the density and vorticity fields to be determined. The vortex ring initially grew, with additional circulation being supplied by the trailing plume. At later times, the vortex ring became disconnected from the trailing plume. This is analogous to the pinch-off of a vortex ring produced by a piston-cylinder apparatus reported by Gharib et al (1998 JFM 360: 121-140). The existence of a pinch-off process for starting buoyant plumes has many implications for environmental flows. Of particular interest is the effect of vortex ring pinch-off on the dispersal of particulates and contaminants in intermittent or sudden convection events.

Arc Evolution in Response to the Subduction of Buoyant Features

NASA Astrophysics Data System (ADS)

Jenkins, Luke; Fourel, Loic; Goes, Saskia; Morra, Gabriele

2015-04-01

The subduction of buoyant features such as aseismic ridges or oceanic plateaux has been invoked to explain arc deformation, flat subduction and increase in seismic coupling. Other studies have challenged these ideas, attributing a larger role to the overriding plate. However, many open questions remain about the dynamics of the relative simple case of a single freely subducting plate. How big does a plateau need to be to change the arc shape? What is the control of plate's strength on the impact of buoyant features? How do the velocities adapt to the subduction of less dense material? In the present study, we propose a systematic approach in order to tackle these questions. We use a new 3-D coupled fluid-solid subduction model where the interaction between the slab and the isoviscous mantle is only calculated on the slab surface, significantly increasing computational efficiency. The oceanic plate rheology is visco-elasto-plastic and its top surface is free. We find that arc shape is significantly altered by the subduction of buoyant plateaux. Along the subduction plane through the plateau and depending on its size, the dip angle and the retreat velocity significantly decrease. Flat subduction is obtained in the case of large and strongly buoyant plateau/ridge. An interesting feature is that retreat velocity increases right after the plateau or ridge has finished subducting in order to catch up with the rest of the plate. The gradient in retreat velocity obtained along the trench may cause the slab to have a heterogeneous response to ridge push, eventually leading to slab advance where buoyant material is present. We apply our models to the Izu-Bonin-Marianas (IBM) trench and propose that subduction of the buoyant Caroline Island Ridge at the southern edge of the Mariana trench can explain both trench motion history and the current morphology of the IBM slab as imaged by seismic tomography.

Preparation of membrane rafts.

PubMed

Waugh, Mark G; Hsuan, J Justin

2009-01-01

The concept that biological membranes contain microdomains of specialized lipid and protein composition has attracted great attention in recent years. Initially, the focus in the field was very much on the characterization of cholesterol-and sphingolipid-rich plasma membrane microdomains that were resistant to solubilization in the cold non-ionic detergent Triton X-100. Such detergent-insoluble membrane domains were of low buoyant density and could be readily purified on sucrose equilibrium density gradients. The intrinsic buoyancy of the detergent-insoluble domains gave rise to the term "lipid rafts." Cholesterol- and sphingolipid-rich rafts at the plasma membrane have been implicated in a wide range of cellular processes, including pathogen invasion, receptor signaling, and endocytosis. However, work with other non-ionic detergents such as Lubrol WX and Brij-98 has revealed the existence of various raft subtypes with differing lipid compositions and proposed functions. More recently, there has been some focus on isolating lipid rafts from intracellular organelles, in particular membranes from the Golgi-endosomal pathway, where raft lipids have been proposed to function in processes such as the sorting of vesicular cargo and the processing of amyloid precursor protein. While there remains a large degree of controversy surrounding the purity, the physiological importance, and even the existence of different types of lipid rafts in intact cells, the ability to routinely purify such domains has led to significant progress in understanding the functional architecture of biological membranes. We describe a number of widely used methods to prepare rafts, based on early preparations of caveolae by density gradient ultracentrifugation and immunoaffinity precipitation.

Observations of a tidal intrusion front in a tidal channel

NASA Astrophysics Data System (ADS)

Lu, Shasha; Xia, Xiaoming; Thompson, Charlie E. L.; Cao, Zhenyi; Liu, Yifei

2017-11-01

A visible front indicated by a surface colour change, and sometimes associated with foam or debris lines, was observed in a tidal channel during neap tide. This is an example of a tidal intrusion front occurring in the absence of sudden topographical changes or reversing flows, typically reported to be associated with such fronts. Detailed Acoustic Doppler Current Profiler and conductivity/temperature/depth measurements were taken on repeated transects both with fronts apparent and with fronts absent. The results indicated that the front occurred as a result of stratification, which was sustained by the buoyancy flux and the weak tide-induced mixing during neap ebb tide. The stronger tide-induced mixing during spring tide restrained stratification, leading to the absence of a front. The mechanism of the frontogenesis was the density gradient between the stratified water formed during neap ebb tide, and the more mixed seawater during neap flood tide; thus, the water on the landward (southwestern) side of the front was stratified, and that on the seaward side (northeastern) of the front was vertically well mixed. Gradient Richardson number estimates suggest that the flow between the stratified and mixed water was near the threshold 0.25 for shear instability. Meanwhile, the density gradient would provide an initial baroclinic contribution to velocity convergence, which is indicated by the accumulation of buoyant matter such as foam, grass, and debris into a sharply defined line along the surface. The front migrates with the flood current, with a local maximum towards the eastern side of the channel, leading to an asymmetrical shape with the eastern side of the front driven further into the Tiaozhoumen tidal channel.

Vortex Ring Formation in a Starting Buoyant Plume

NASA Astrophysics Data System (ADS)

Pottebaum, Tait; Shusser, Michael; Gharib, Morteza

1999-11-01

Vortex ring formation in starting buoyant plumes is studied experimentally. Buoyant plumes are produced using a heating element at the base of a water tank. Digital particle image velocimetry and thermometry (DPIVT) is used to obtain the velocity and temperature fields, from which the vorticity and density fields are determined. The results indicate that the circulation of the vortex ring initially grows and saturates at later times. This saturation process is associated with the disconnection of the vorticity field of the vortex ring from that of the trailing plume. This is analogous to the pinch off of a vortex ring produced by a piston as reported by Gharib et al (1998 JFM 360, 121-140). Similar to the definition used by Gharib et al, a 'formation number' can be defined as the normalized time at which the circulation produced by the buoyancy source is equal to the peak circulation achieved by the vortex ring. This formation number is examined for a variety of plume density ratios. The results are compared to predictions of a model based on the Kelvin-Benjamin variational principle for steady axis-touching vortex rings.

Use of a Force Sensor in Archimedes' Principle Experiment, Determination of Buoyant Force and Acceleration Due To Gravity

NASA Astrophysics Data System (ADS)

Aurora, Tarlok

2013-04-01

In introductory physics, students verify Archimedes' principle by immersing an object in water in a container, with a side-spout to collect the displaced water, resulting in a large uncertainty, due to surface tension. A modified procedure was introduced, in which a plastic bucket is suspended from a force sensor, and an object hangs underneath the bucket. The object is immersed in water in a glass beaker (without any side spout), and the weight loss is measured with a computer-controlled force sensor. Instead of collecting the water displaced by the object, tap water was added to the bucket to compensate for the weight loss, and the Archimedes' principle was verified within less than a percent. With this apparatus, buoyant force was easily studied as a function of volume of displaced water; as well as a function of density of saline solution. By graphing buoyant force as a function of volume (or density of liquid), value of g was obtained from slope. Apparatus and sources of error will be discussed.

Differential force balances during levitation

NASA Astrophysics Data System (ADS)

Todd, Paul

The simplest arithmetic of inertial, buoyant, magnetic and electrokinetic levitation is explored in the context of a model living system with “acceleration-sensitive structures” in which motion, if allowed, produces a biological effect. The simple model is a finite-sized object enclosed within another finite-sized object suspended in an outer fluid (liquid or vapor) medium. The inner object has density and electrical and magnetic properties quantitatively different from those of the outer object and the medium. In inertial levitation (“weightlessness”) inertial accelerations are balanced, and the forces due to them are canceled in accordance with Newton’s third law. In the presence of inertial acceleration (gravity, centrifugal) motionlessness depends on a balance between the levitating force and the inertial force. If the inner and outer objects differ in density one or the other will be subjected to an unbalanced force when one object is levitated by any other force (buoyant, magnetic, electrokinetic). The requirements for motionlessness of the internal object in the presence of a levitating force are equality of density in the case of buoyant levitation, equality of magnetic susceptibility in the case of magnetic levitation, and equality of zeta potential and dielectric constant in the case of electrokinetic levitation. Examples of internal “acceleration-sensitive structures” are cellular organelles and the organs of advanced plants and animals. For these structures fundamental physical data are important in the interpretation of the effects of forces used for levitation.

Surface winds over West Antarctica

NASA Astrophysics Data System (ADS)

Bromwich, David

1993-07-01

Five winter months (April-August 1988) of thermal infrared satellite images were examined to investigate the occurrence of dark (warm) signatures across the Ross Ice Shelf in the Antarctic continent. These features are inferred to be generated by katabatic winds that descend from southern Marie Byrd Land and then blow horizontally across the ice shelf. Significant mass is added to this airstream by katabatic winds blowing from the major glaciers that flow through the Transantarctic Mountains from East Antarctica. These negatively buoyant katabatic winds can reach the northwestern edge of the shelf - a horizontal propagation distance of up to 1,000 km - 14 percent of the time. Where the airstream crosses from the ice shelf to the ice-covered Ross Sea, a prominent coastal polynya is formed. Because the downslope buoyancy force is near zero over the Ross Ice Shelf, the northwestward propagation of the katabatic air mass requires pressure gradient support. The study shows that the extended horizontal propagation of this atmospheric density current occurred in conjunction with the passage of synoptic cyclones over the southern Amundsen Sea. These cyclones can strengthen the pressure gradient in the interior of West Antarctica and make the pressure field favorable for northwestward movement of the katabatic winds from West Antarctica across the ice shelf in a geostrophic direction. The glacier winds from East Antarctica are further accelerated by the synoptic pressure gradient, usually undergo abrupt adjustment beyond the exit to the glacier valley, and merge into the mountain-parallel katabatic air mass.

Wake-Driven Dynamics of Finite-Sized Buoyant Spheres in Turbulence

NASA Astrophysics Data System (ADS)

Mathai, Varghese; Prakash, Vivek N.; Brons, Jon; Sun, Chao; Lohse, Detlef

2015-09-01

Particles suspended in turbulent flows are affected by the turbulence and at the same time act back on the flow. The resulting coupling can give rise to rich variability in their dynamics. Here we report experimental results from an investigation of finite-sized buoyant spheres in turbulence. We find that even a marginal reduction in the particle's density from that of the fluid can result in strong modification of its dynamics. In contrast to classical spatial filtering arguments and predictions of particle models, we find that the particle acceleration variance increases with size. We trace this reversed trend back to the growing contribution from wake-induced forces, unaccounted for in current particle models in turbulence. Our findings highlight the need for improved multiphysics based models that account for particle wake effects for a faithful representation of buoyant-sphere dynamics in turbulence.

Behavior of a wave-driven buoyant surface jet on a coral reef

USGS Publications Warehouse

Herdman, Liv; Hench, James L.; Fringer, Oliver; Monismith, Stephen G.

2017-01-01

A wave-driven surface buoyant jet exiting a coral reef was studied in order to quantify the amount of water re-entrained over the reef crest. Both moored observations and Lagrangian drifters were used to study the fate of the buoyant jet. To investigate in detail the effects of buoyancy and along-shore flow variations, we developed an idealized numerical model of the system. Consistent with previous work, the ratio of along-shore velocity to jet-velocity and the jet internal Froude number were found to be important determinants of the fate of the jet. In the absence of buoyancy, the entrainment of fluid at the reef crest, creates a significant amount of retention, keeping 60% of water in the reef system. However, when the jet is lighter than the ambient ocean-water, the net effect of buoyancy is to enhance the separation of the jet from shore, leading to a greater export of reef water. Matching observations, our modeling predicts that buoyancy limits retention to 30% of the jet flow for conditions existing on the Moorea reef. Overall, the combination of observations and modeling we present here shows that reef-ocean temperature gradients can play an important role in reef-ocean exchanges.

Behavior of a wave-driven buoyant surface jet on a coral reef

NASA Astrophysics Data System (ADS)

Herdman, Liv M. M.; Hench, James L.; Fringer, Oliver; Monismith, Stephen G.

2017-05-01

A wave-driven surface-buoyant jet exiting a coral reef was studied in order to quantify the amount of water reentrained over the reef crest. Both moored observations and Lagrangian drifters were used to study the fate of the buoyant jet. To investigate in detail the effects of buoyancy and alongshore flow variations, we developed an idealized numerical model of the system. Consistent with previous work, the ratio of alongshore velocity to jet velocity and the jet internal Froude number were found to be important determinants of the fate of the jet. In the absence of buoyancy, the entrainment of fluid at the reef crest creates a significant amount of retention, keeping 60% of water in the reef system. However, when the jet is lighter than the ambient ocean water, the net effect of buoyancy is to enhance the separation of the jet from shore, leading to a greater export of reef water. Matching observations, our modeling predicts that buoyancy limits retention to 30% of the jet flow for conditions existing on the Moorea reef. Overall, the combination of observations and modeling we present here shows that reef-ocean temperature gradients can play an important role in reef-ocean exchanges.

Explosive Volcanic Eruptions from Linear Vents on Earth, Venus and Mars: Comparisons with Circular Vent Eruptions

NASA Technical Reports Server (NTRS)

Glaze, Lori S.; Baloga, Stephen M.; Wimert, Jesse

2010-01-01

Conditions required to support buoyant convective plumes are investigated for explosive volcanic eruptions from circular and linear vents on Earth, Venus, and Mars. Vent geometry (linear versus circular) plays a significant role in the ability of an explosive eruption to sustain a buoyant plume. On Earth, linear and circular vent eruptions are both capable of driving buoyant plumes to equivalent maximum rise heights, however, linear vent plumes are more sensitive to vent size. For analogous mass eruption rates, linear vent plumes surpass circular vent plumes in entrainment efficiency approximately when L(sub o) > 3r(sub o) owing to the larger entrainment area relative to the control volume. Relative to circular vents, linear vents on Venus favor column collapse and the formation of pyroclastic flows because the range of conditions required to establish and sustain buoyancy is narrow. When buoyancy can be sustained, however, maximum plume heights exceed those from circular vents. For current atmospheric conditions on Mars, linear vent eruptions are capable of injecting volcanic material slightly higher than analogous circular vent eruptions. However, both geometries are more likely to produce pyroclastic fountains, as opposed to convective plumes, owing to the low density atmosphere. Due to the atmospheric density profile and water content on Earth, explosive eruptions enjoy favorable conditions for producing sustained buoyant columns, while pyroclastic flows would be relatively more prevalent on Venus and Mars. These results have implications for the injection and dispersal of particulates into the planetary atmosphere and the ability to interpret the geologic record of planetary volcanism.

Coupling between Buoyancy Forces and Electroconvective Instability near Ion-Selective Surfaces.

PubMed

Karatay, Elif; Andersen, Mathias Bækbo; Wessling, Matthias; Mani, Ali

2016-05-13

Recent investigations have revealed that ion transport from aqueous electrolytes to ion-selective surfaces is subject to electroconvective instability that stems from coupling of hydrodynamics with electrostatic forces. These systems inherently involve fluid density variation set by salinity gradients. However, the coupling between the buoyancy effects and electroconvective instability has not yet been investigated although a wide range of electrochemical systems are naturally prone to these interplaying effects. In this study we thoroughly examine the interplay of gravitational convection and chaotic electroconvection. Our results reveal that buoyant forces can significantly influence the transport rates, otherwise set by electroconvection, when the Rayleigh number Ra of the system exceeds a value Ra∼1000. We show that buoyancy forces can significantly alter the flow patterns in these systems. When the buoyancy acts in the stabilizing direction, it limits the extent of penetration of electroconvection, but without eliminating it. When the buoyancy destabilizes the flow, it alters the electroconvective patterns by introducing upward and downward fingers of respectively light and heavy fluids.

Rotational effect of buoyancy in frontcrawl: Does it really cause the legs to sink?

PubMed

Yanai, T

2001-02-01

The purposes of this study were to quantify the rotational effect of buoyant force (buoyant torque) during the performance of front crawl and to reexamine the mechanics of horizontal alignment of the swimmers. Three-dimensional videography was used to measure the position and orientation of the body segments of 11 competitive swimmers performing front crawl stroke at a sub-maximum sprinting speed. The dimensions of each body segment were defined mathematically to match the body segment parameters (mass, density, and centroid position) reported in the literature. The buoyant force and torque were computed for every video-field (60fields/s), assuming that the water surface followed a sine curve along the length of the swimmer. The average buoyant torque over the stroke cycle (mean=22Nm) was directed to raise the legs and lower the head, primarily because the recovery arm and a part of the head were lifted out of the water and the center of buoyancy shifted toward the feet. This finding contradicts the prevailing speculation that buoyancy only causes the legs to sink throughout the stroke cycle. On the basis of a theoretical analysis of the results, it is postulated that the buoyant torque, and perhaps the forces generated by kicks, function to counteract the torque generated by the hydrodynamic forces acting on the hands, so as to maintain the horizontal alignment of the body in front crawl.

A study of the accuracy of neutrally buoyant bubbles used as flow tracers in air

NASA Technical Reports Server (NTRS)

Kerho, Michael F.

1993-01-01

Research has been performed to determine the accuracy of neutrally buoyant and near neutrally buoyant bubbles used as flow tracers in air. Theoretical, computational, and experimental results are presented to evaluate the dynamics of bubble trajectories and factors affecting their ability to trace flow-field streamlines. The equation of motion for a single bubble was obtained and evaluated using a computational scheme to determine the factors which affect a bubble's trajectory. A two-dimensional experiment was also conducted to experimentally determine bubble trajectories in the stagnation region of NACA 0012 airfoil at 0 deg angle of attack using a commercially available helium bubble generation system. Physical properties of the experimental bubble trajectories were estimated using the computational scheme. These properties included the density ratio and diameter of the individual bubbles. the helium bubble system was then used to visualize and document the flow field about a 30 deg swept semispan wing with simulated glaze ice. Results were compared to Navier-Stokes calculations and surface oil flow visualization. The theoretical and computational analysis have shown that neutrally buoyant bubbles will trace even the most complex flow patterns. Experimental analysis revealed that the use of bubbles to trace flow patterns should be limited to qualitative measurements unless care is taken to ensure neutral buoyancy. This is due to the difficulty in the production of neutrally buoyant bubbles.

Rayleigh-Taylor Gravity Waves and Quasiperiodic Oscillation Phenomenon in X-ray Binaries

NASA Technical Reports Server (NTRS)

Titarchuk, Lev

2002-01-01

Accretion onto compact objects in X-ray binaries (black hole, neutron star (NS), white dwarf) is characterized by non-uniform flow density profiles. Such an effect of heterogeneity in presence of gravitational forces and pressure gradients exhibits Rayleigh-Taylor gravity waves (RTGW). They should be seen as quasiperiodic wave oscillations (QPO) of the accretion flow in the transition (boundary) layer between the Keplerian disk and the central object. In this paper the author shows that the main QPO frequency, which is very close to the Keplerian frequency, is split into separate frequencies (hybrid and low branch) under the influence of the gravitational forces in the rotational frame of reference. The RTGWs must be present and the related QPOs should be detected in any system where the gravity, buoyancy and Coriolis force effects cannot be excluded (even in the Earth and solar environments). The observed low and high QPO frequencies are an intrinsic signature of the RTGW. The author elaborates the conditions for the density profile when the RTGW oscillations are stable. A comparison of the inferred QPO frequencies with QPO observations is presented. The author finds that hectohertz frequencies detected from NS binaries can be identified as the RTGW low branch frequencies. The author also predicts that an observer can see the double NS spin frequency during the NS long (super) burst events when the pressure gradients and buoyant forces are suppressed. The Coriolis force is the only force which acts in the rotational frame of reference and its presence causes perfect coherent pulsations with a frequency twice of the NS spin. The QPO observations of neutron binaries have established that the high QPO frequencies do not go beyond of the certain upper limit. The author explains this observational effect as a result of the density profile inversions. Also the author demonstrates that a particular problem of the gravity waves in the rotational frame of reference in the approximation of very small pressure gradients is reduced to the problem of the classical oscillator in the rotational frame of reference which was previously introduced and applied for the interpretation of kHZ QPO observation by Osherovich & Titarchuk.

Neutrally buoyant tracers in hydrogeophysics: Field demonstration in fractured rock

NASA Astrophysics Data System (ADS)

Shakas, Alexis; Linde, Niklas; Baron, Ludovic; Selker, John; Gerard, Marie-Françoise; Lavenant, Nicolas; Bour, Olivier; Le Borgne, Tanguy

2017-04-01

Electrical and electromagnetic methods are extensively used to map electrically conductive tracers within hydrogeologic systems. Often, the tracers used consist of dissolved salt in water, leading to a denser mixture than the ambient formation water. Density effects are often ignored and rarely modeled but can dramatically affect transport behavior and introduce dynamics that are unrepresentative of the response obtained with classical tracers (e.g., uranine). We introduce a neutrally buoyant tracer consisting of a mixture of salt, water, and ethanol and monitor its movement during push-pull experiments in a fractured rock aquifer using ground-penetrating radar. Our results indicate a largely reversible transport process and agree with uranine-based push-pull experiments at the site, which is in contrast to results obtained using dense saline tracers. We argue that a shift toward neutrally buoyant tracers in both porous and fractured media would advance hydrogeophysical research and enhance its utility in hydrogeology.

Bed failure induced by internal solitary waves

NASA Astrophysics Data System (ADS)

Rivera-Rosario, Gustavo A.; Diamessis, Peter J.; Jenkins, James T.

2017-07-01

The pressure field inside a porous bed induced by the passage of an Internal Solitary Wave (ISW) of depression is examined using high-accuracy numerical simulations. The velocity and density fields are obtained by solving the Dubreil-Jacotin-Long Equation, for a two-layer, continuously stratified water column. The total wave-induced pressure across the surface of the bed is computed by vertically integrating for the hydrostatic and nonhydrostatic contributions. The bed is assumed to be a continuum composed of either sand or silt, with a small amount of trapped gas. Results show variations in pore-water pressure penetrating deeper into more conductive materials and remaining for a prolonged period after the wave has passed. In order to quantify the potential for failure, the vertical pressure gradient is compared against the buoyant weight of the bed. The pressure gradient exceeds this weight for weakly conductive materials. Failure is further enhanced by a decrease in bed saturation, consistent with studies in surface-wave induced failure. In deeper water, the ISW-induced pressure is stronger, causing failure only for weakly conductive materials. The pressure associated with the free-surface displacement that accompanies ISWs is significant, when the water depth is less than 100 m, but has little influence when it is greater than 100 m, where the hydrostatic pressure due to the pycnocline displacement is much larger. Since the pore-pressure gradient reduces the specific weight of the bed, results show that particles are easier for the flow to suspend, suggesting that pressure contributes to the powerful resuspension events observed in the field.

Specific binding of sup 125 I-rErythropoietin to Friend polycythemia virus-transformed erythroleukemia cells purified by centrifugal elutriation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Correa, P.N.; Bard, V.; Axelrad, A.A.

1990-01-01

We have used countercurrent centrifugal elutriation (CCE) to determine the distribution of cells with respect to cell volume and buoyant density for an erythroleukemia cell line (JG6) transformed by the polycythemia strain of Friend virus (FV-P), and to determine the effect of inducing the cells to differentiate with dimethylsulfoxide (DMSO) on this distribution. CCE made it possible to obtain suspensions of modal JG6 populations virtually free of dead cells and uniform with respect to volume and buoyant density. These modal populations were assayed for specific binding of erythropoietin (Epo). Between 500 and 550 Epo receptors per cell were detected. Thesemore » belonged to a single class having a dissociation constant of 0.36 nM. DMSO induction of differentiation of the JG6 cells had no effect on the number of Epo receptors expressed.« less

Influence of roughness bottom on the dynamics of a buoyant cloud : application to a powder avalanche

NASA Astrophysics Data System (ADS)

Brossard, D.; Naaim-Bouvet, F.; Naaim, M.; Caccamo, P.

2009-04-01

A powder avalanche is referred to as a turbulent flow of snow particles in air. In the past such avalanches have been modelled by buoyant cloud in a watertank: buoyant clouds flow along an inclined plane from a small immersed tank with a release gate (injection is of short duration). The powder avalanches are simulated by a heavy fluid (salt water + colorant or kaolin) which is dispersing in a lighter one. Such experiments allow studies for the influence of roughness bottoms on the dynamics of a buoyant clouds. The authors studied the flows of buoyant clouds on an uniform slope of 20° with different roughness: smooth PVC, abrasive paper, bottom covered with glued particles of PMMA or with glued glass beads of different sizes arranged in a compact way. The released volume varies between 2 to 4 liters and the density of salted water is 1.2. Two cameras are used to obtain the height together with the front velocity. Inside the study area the front velocity is approximately constant and the height of the clouds varies linearly with the distance from the released gate as usually observed in previous experiments. So for each roughness a front velocity and height growth can be defined. It was shown from the experiments that: As the bottom increases in roughness, the front speed increases and the height growth decreases. Nevertheless the height of glued elements does not seem to be the most appropriate parameter to characterize the roughness.

Similar solutions of double-diffusive dissipative layers along free surfaces

NASA Astrophysics Data System (ADS)

Napolitano, L. G.; Viviani, A.; Savino, R.

1990-10-01

Free convection due to buoyant forces (natural convection) and surface tension gradients (Marangoni convection) generated by temperature and concentration gradients is discussed together with the formation of double-diffusive boundary layers along liquid-gas interfaces. Similarity solutions for each class of free convection are derived and the resulting nonlinear two-point problems are solved numerically using the quasi-linearization method. Velocity, temperature, concentration profiles, interfacial velocity, heat and mass transfer bulk coefficients for various Prandtl and Schmidt numbers, and different values of the similarity parameters are determined. The convective flows are of particular interest because they are considered to influence the processes of crystal growth, both on earth and in a microgravity environment.

Lifestyle and Metformin Treatment Favorably Influence Lipoprotein Subfraction Distribution in the Diabetes Prevention Program

PubMed Central

Temprosa, M.; Otvos, J.; Brunzell, J.; Marcovina, S.; Mather, K.; Arakaki, R.; Watson, K.; Horton, E.; Barrett-Connor, E.

2013-01-01

Context: Although intensive lifestyle change (ILS) and metformin reduce diabetes incidence in subjects with impaired glucose tolerance (IGT), their effects on lipoprotein subfractions have not been studied. Objective: The objective of the study was to characterize the effects of ILS and metformin vs placebo interventions on lipoprotein subfractions in the Diabetes Prevention Program. Design: This was a randomized clinical trial, testing the effects of ILS, metformin, and placebo on diabetes development in subjects with IGT. Participants: Selected individuals with IGT randomized in the Diabetes Prevention Program participated in the study. Interventions: Interventions included randomization to metformin 850 mg or placebo twice daily or ILS aimed at a 7% weight loss using a low-fat diet with increased physical activity. Main Outcome Measures: Lipoprotein subfraction size, density, and concentration measured by magnetic resonance and density gradient ultracentrifugation at baseline and 1 year were measured. Results: ILS decreased large and buoyant very low-density lipoprotein, small and dense low-density lipoprotein (LDL), and small high-density lipoprotein (HDL) and raised large HDL. Metformin modestly reduced small and dense LDL and raised small and large HDL. Change in insulin resistance largely accounted for the intervention-associated decreases in large very low-density lipoprotein, whereas changes in body mass index (BMI) and adiponectin were strongly associated with changes in LDL. Baseline and a change in adiponectin were related to change in large HDL, and BMI change associated with small HDL change. The effect of metformin to increase small HDL was independent of adiponectin, BMI, and insulin resistance. Conclusion: ILS and metformin treatment have favorable effects on lipoprotein subfractions that are primarily mediated by intervention-related changes in insulin resistance, BMI, and adiponectin. Interventions that slow the development of diabetes may also retard the progression of atherosclerosis. PMID:23979954

Physical trade-offs shape the evolution of buoyancy control in sharks.

PubMed

Gleiss, Adrian C; Potvin, Jean; Goldbogen, Jeremy A

2017-11-15

Buoyancy control is a fundamental aspect of aquatic life that has major implications for locomotor performance and ecological niche. Unlike terrestrial animals, the densities of aquatic animals are similar to the supporting fluid, thus even small changes in body density may have profound effects on locomotion. Here, we analysed the body composition (lipid versus lean tissue) of 32 shark species to study the evolution of buoyancy. Our comparative phylogenetic analyses indicate that although lean tissue displays minor positive allometry, liver volume exhibits pronounced positive allometry, suggesting that larger sharks evolved bulkier body compositions by adding lipid tissue to lean tissue rather than substituting lean for lipid tissue, particularly in the liver. We revealed a continuum of buoyancy control strategies that ranged from more buoyant sharks with larger livers in deeper ecosystems to relatively denser sharks with small livers in epipelagic habitats. Across this eco-morphological spectrum, our hydrodynamic modelling suggests that neutral buoyancy yields lower drag and more efficient steady swimming, whereas negative buoyancy may be more efficient during accelerated movements. The evolution of buoyancy control in sharks suggests that ecological and physiological factors mediate the selective pressures acting on these traits along two major gradients, body size and habitat depth. © 2017 The Author(s).

Membrane-association of mRNA decapping factors is independent of stress in budding yeast

PubMed Central

Huch, Susanne; Gommlich, Jessie; Muppavarapu, Mridula; Beckham, Carla; Nissan, Tracy

2016-01-01

Recent evidence has suggested that the degradation of mRNA occurs on translating ribosomes or alternatively within RNA granules called P bodies, which are aggregates whose core constituents are mRNA decay proteins and RNA. In this study, we examined the mRNA decapping proteins, Dcp1, Dcp2, and Dhh1, using subcellular fractionation. We found that decapping factors co-sediment in the polysome fraction of a sucrose gradient and do not alter their behaviour with stress, inhibition of translation or inhibition of the P body formation. Importantly, their localisation to the polysome fraction is independent of the RNA, suggesting that these factors may be constitutively localised to the polysome. Conversely, polysomal and post-polysomal sedimentation of the decapping proteins was abolished with the addition of a detergent, which shifts the factors to the non-translating RNP fraction and is consistent with membrane association. Using a membrane flotation assay, we observed the mRNA decapping factors in the lower density fractions at the buoyant density of membrane-associated proteins. These observations provide further evidence that mRNA decapping factors interact with subcellular membranes, and we suggest a model in which the mRNA decapping factors interact with membranes to facilitate regulation of mRNA degradation. PMID:27146487

Membrane-association of mRNA decapping factors is independent of stress in budding yeast.

PubMed

Huch, Susanne; Gommlich, Jessie; Muppavarapu, Mridula; Beckham, Carla; Nissan, Tracy

2016-05-05

Recent evidence has suggested that the degradation of mRNA occurs on translating ribosomes or alternatively within RNA granules called P bodies, which are aggregates whose core constituents are mRNA decay proteins and RNA. In this study, we examined the mRNA decapping proteins, Dcp1, Dcp2, and Dhh1, using subcellular fractionation. We found that decapping factors co-sediment in the polysome fraction of a sucrose gradient and do not alter their behaviour with stress, inhibition of translation or inhibition of the P body formation. Importantly, their localisation to the polysome fraction is independent of the RNA, suggesting that these factors may be constitutively localised to the polysome. Conversely, polysomal and post-polysomal sedimentation of the decapping proteins was abolished with the addition of a detergent, which shifts the factors to the non-translating RNP fraction and is consistent with membrane association. Using a membrane flotation assay, we observed the mRNA decapping factors in the lower density fractions at the buoyant density of membrane-associated proteins. These observations provide further evidence that mRNA decapping factors interact with subcellular membranes, and we suggest a model in which the mRNA decapping factors interact with membranes to facilitate regulation of mRNA degradation.

Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes.

PubMed

Vetrivel, Kulandaivelu S; Cheng, Haipeng; Lin, William; Sakurai, Takashi; Li, Tong; Nukina, Nobuyuki; Wong, Philip C; Xu, Huaxi; Thinakaran, Gopal

2004-10-22

Alzheimer's disease-associated beta-amyloid peptides (Abeta) are generated by the sequential proteolytic processing of amyloid precursor protein (APP) by beta- and gamma-secretases. There is growing evidence that cholesterol- and sphingolipid-rich membrane microdomains are involved in regulating trafficking and processing of APP. BACE1, the major beta-secretase in neurons is a palmitoylated transmembrane protein that resides in lipid rafts. A subset of APP is subject to amyloidogenic processing by BACE1 in lipid rafts, and this process depends on the integrity of lipid rafts. Here we describe the association of all four components of the gamma-secretase complex, namely presenilin 1 (PS1)-derived fragments, mature nicastrin, APH-1, and PEN-2, with cholesterol-rich detergent insoluble membrane (DIM) domains of non-neuronal cells and neurons that fulfill the criteria of lipid rafts. In PS1(-/-)/PS2(-/-) and NCT(-/-) fibroblasts, gamma-secretase components that still remain fail to become detergent-resistant, suggesting that raft association requires gamma-secretase complex assembly. Biochemical evidence shows that subunits of the gamma-secretase complex and three TGN/endosome-resident SNAREs cofractionate in sucrose density gradients, and show similar solubility or insolubility characteristics in distinct non-ionic and zwitterionic detergents, indicative of their co-residence in membrane microdomains with similar protein-lipid composition. This notion is confirmed using magnetic immunoisolation of PS1- or syntaxin 6-positive membrane patches from a mixture of membranes with similar buoyant densities following Lubrol WX extraction or sonication, and gradient centrifugation. These findings are consistent with the localization of gamma-secretase in lipid raft microdomains of post-Golgi and endosomes, organelles previously implicated in amyloidogenic processing of APP.

Association of γ-Secretase with Lipid Rafts in Post-Golgi and Endosome Membranes*

PubMed Central

Vetrivel, Kulandaivelu S.; Cheng, Haipeng; Lin, William; Sakurai, Takashi; Li, Tong; Nukina, Nobuyuki; Wong, Philip C.; Xu, Huaxi; Thinakaran, Gopal

2005-01-01

Alzheimer’s disease-associated β-amyloid peptides (Aβ) are generated by the sequential proteolytic processing of amyloid precursor protein (APP) by β- and γ-secretases. There is growing evidence that cholesterol- and sphingolipid-rich membrane microdomains are involved in regulating trafficking and processing of APP. BACE1, the major γ-secretase in neurons is a palmi-toylated transmembrane protein that resides in lipid rafts. A subset of APP is subject to amyloidogenic processing by BACE1 in lipid rafts, and this process depends on the integrity of lipid rafts. Here we describe the association of all four components of the γ-secretase complex, namely presenilin 1 (PS1)-derived fragments, mature nicastrin, APH-1, and PEN-2, with cholesterol-rich detergent insoluble membrane (DIM) domains of non-neuronal cells and neurons that fulfill the criteria of lipid rafts. In PS1−/−/PS2−/− and NCT−/− fibroblasts, γ-secretase components that still remain fail to become detergent-resistant, suggesting that raft association requires γ-secretase complex assembly. Biochemical evidence shows that subunits of the γ-secretase complex and three TGN/endosome-resident SNAREs cofractionate in sucrose density gradients, and show similar solubility or insolubility characteristics in distinct non-ionic and zwitterionic detergents, indicative of their co-residence in membrane microdomains with similar protein-lipid composition. This notion is confirmed using magnetic immunoisolation of PS1- or syntaxin 6-positive membrane patches from a mixture of membranes with similar buoyant densities following Lubrol WX extraction or sonication, and gradient centrifugation. These findings are consistent with the localization of γ-secretase in lipid raft microdomains of post-Golgi and endosomes, organelles previously implicated in amyloidogenic processing of APP. PMID:15322084

DNA of a Human Hepatitis B Virus Candidate

PubMed Central

Robinson, William S.; Clayton, David A.; Greenman, Richard L.

1974-01-01

Particles containing DNA polymerase (Dane particles) were purified from the plasma of chronic carriers of hepatitis B antigen. After a DNA polymerase reaction with purified Dane particle preparations treated with Nonidet P-40 detergent, Dane particle core structures containing radioactive DNA product were isolated by sedimentation in a sucrose density gradient. The radioactive DNA was extracted with sodium dodecyl sulfate and isolated by band sedimentation in a preformed CsCl gradient. Examination of the radioactive DNA band by electron microscopy revealed exclusively circular double-stranded DNA molecules approximately 0.78 μm in length. Identical circular molecules were observed when DNA was isolated by a similar procedure from particles that had not undergone a DNA polymerase reaction. The molecules were completely degraded by DNase 1. When Dane particle core structures were treated with DNase 1 before DNA extraction, only 0.78-μm circular DNA molecules were detected. Without DNase treatment of core structures, linear molecules with lengths between 0.5 and 12 μm, in addition to the 0.78-μm circles were found. These results suggest that the 0.78-μm circular molecules were in a protected position within Dane particle cores and the linear molecules were not within core structures. Length measurements on 225 circular molecules revealed a mean length of 0.78 ± 0.09 μm which would correspond to a molecular weight of around 1.6 × 106. The circular molecules probably serve as primer-template for the DNA polymerase reaction carried out by Dane particle cores. Thermal denaturation and buoyant density measurements on the Dane particle DNA polymerase reaction product revealed a guanosine plus cytosine content of 48 to 49%. Images PMID:4847328

Phosphoinositide 3-kinase regulates maturation of lysosomes in rat hepatocytes.

PubMed Central

Mousavi, Seyed Ali; Brech, Andreas; Berg, Trond; Kjeken, Rune

2003-01-01

To obtain information about the role of phosphoinositide 3-kinase (PI3K) in the endocytic pathway in hepatocytes, the uptake and intracellular transport of asialo-orosomucoid (ASOR) was followed in cells treated with wortmannin or LY294002. The two inhibitors, at concentrations known to inhibit the enzyme, did not affect internalization or the number of surface asialoglycoprotein receptors, but they caused a paradoxical increase (approx. 50% above control values) in the degradation of ASOR labelled with [(125)I]tyramine cellobiose ([(125)I]TC). Wortmannin or LY204002 inhibited the autophagic sequestration of lactate dehydrogenase very effectively, and the enhanced degradation of [(125)I]TC-ASOR could be an indirect effect of reduced autophagy, as an amino acid mixture known to inhibit autophagy also caused increased degradation of [(125)I]TC-ASOR, and its effect was not additive to that of wortmannin or LY294002. Wortmannin or LY294002 had pronounced effects on the late parts of the endocytic pathway in the hepatocytes: first, dense lysosomes disappeared and were replaced by swollen vesicles; secondly, degradation of [(125)I]TC-ASOR took place in an organelle of lower buoyant density (in a sucrose gradient) than the bulk of lysosomes (identified in the gradient by lysosomal marker enzymes). With increasing length of incubation with wortmannin or LY294002, the density distributions of the lysosomal markers also shifted to lower density and gradually approached that of the labelled degradation products. The labelled degradation products formed from [(125)I]TC-labelled proteins were trapped at the site of formation, because they did not penetrate the vesicle membranes. The results obtained indicate that internalization and intracellular transport of ASOR to lysomes may take place in the absence of PI3K activity in rat hepatocytes. On the other hand, fusion of late endosomes with lysosomes seems to produce 'hybrid organelles' (active lysosomes) that are unable to mature into dense lysosomes. PMID:12646047

The support of long wavelength loads on Venus

NASA Astrophysics Data System (ADS)

Benerdt, W. B.; Saunders, R. S.

1985-04-01

One of the great surprises of the Pioneer Venus mission was the high degree of correlation between topography and gravity found at all wavelengths. This implies a close relationship between topography and lateral subsurface density anomalies, such as those due to passive or dynamic compensation. Sleep-Phillips type compensation model with a variable crustal thickness and a variable upper mantle density was developed. The thin shell theory was used to investigate three end member cases: (1) loading by topographic construction, resulting in a downward deflection of the surface (no mantle support); (2) completely compensated support of a constructional load (no surface deflection); and (3) topography due entirely to upward deflection of the surface supported by a low density upper mantle (no surface load). In general, the models imply relatively thick crust and dense upper mantle for Ishtar Terra and Ovda Regio (western Aphrodite), thinned crust and buoyant upper mantle for Tethus Regio and regions near Sappho and Alpha Regio, and a nearly uniform crust with a buoyant upper mantle for Beta Regio and Atla Regio (eastern Aphrodite).

The Support of Long Wavelength Loads on Venus

NASA Technical Reports Server (NTRS)

Benerdt, W. B.; Saunders, R. S.

1985-01-01

One of the great surprises of the Pioneer Venus mission was the high degree of correlation between topography and gravity found at all wavelengths. This implies a close relationship between topography and lateral subsurface density anomalies, such as those due to passive or dynamic compensation. Sleep-Phillips type compensation model with a variable crustal thickness and a variable upper mantle density was developed. The thin shell theory was used to investigate three end member cases: (1) loading by topographic construction, resulting in a downward deflection of the surface (no mantle support); (2) completely compensated support of a constructional load (no surface deflection); and (3) topography due entirely to upward deflection of the surface supported by a low density upper mantle (no surface load). In general, the models imply relatively thick crust and dense upper mantle for Ishtar Terra and Ovda Regio (western Aphrodite), thinned crust and buoyant upper mantle for Tethus Regio and regions near Sappho and Alpha Regio, and a nearly uniform crust with a buoyant upper mantle for Beta Regio and Atla Regio (eastern Aphrodite).

Spatial patterns of water-dispersed seed deposition along stream riparian gradients

PubMed Central

Moinier, Sophie; van Gogh, Iris; Timmers, Robert; van Deelen, Joost J.; Verhoeven, Jos T. A.; Soons, Merel B.

2017-01-01

Riparian ecosystems along streams naturally harbour a high plant diversity with many increasingly endangered species. In our current heavily modified and fragmented catchments, many of these species are sensitive to dispersal limitation. Better understanding of riparian plant dispersal pathways is required to predict species (re-)colonization potential and improve success rates of stream and riparian zone conservation and restoration. Dispersal by water (hydrochory) is an important mechanism for longitudinal and lateral dispersal of riparian species. Crucially for recruitment potential, it also influences the elevation along the riparian hydrological gradient where seeds become deposited. Due to the complex interplay between abiotic and biotic factors, however, it remains unclear how exactly patterns in seed deposition are formed. We compared hydrochorous and non-hydrochorous seed deposition, and quantified patterns of seed deposition along the bare substrate of newly created stream riparian gradients. Water levels were monitored and seed deposition was measured with seed traps along the full range of riparian hydrological conditions (from permanently flooded to never flooded). Average seed numbers and species richness were significantly higher in flooded than in non-flooded seed traps (5.7 and 1.5 times higher, respectively). Community-weighted trait means indicated that typically water-dispersed seeds were more dominant in flooded than in non-flooded seed traps and gradually decreased in concentration from the channel to the upland. Moreover, highly buoyant seeds accumulated at the average water line, and clear elevational sorting of non-buoyant seeds occurred within the floodplain. These results establish a critical role of flooding in shaping patterns of seed deposition along the riparian gradient, delivering many seeds of typical riparian species to riparian zones and depositing them at species-specific elevations as influenced by seed traits, suggesting species-specific dispersal pathways. This shows that hydrochory likely has important consequences for riparian vegetation development and that flooding forms a key process for successful restoration. PMID:28957365

Characterizing the Severe Turbulence Environments Associated with Commercial Aviation Accidents. Part 2; Hydrostatic Mesobeta Scale Numerical Simulations of Supergradient Wind Flow and Streamwise Ageostrophic Frontogenesis

NASA Technical Reports Server (NTRS)

Kaplan, Michael L.; Huffman, Allan W.; Lux, Kevin M.; Cetola, Jeffrey D.; Charney, Joseph J.; Riordan, Allen J.; Lin, Yuh-Lang; Waight, Kenneth T., III; Proctor, Fred (Technical Monitor)

2003-01-01

Simulation experiments reveal key processes that organize a hydrostatic environment conducive to severe turbulence. The paradigm requires juxtaposition of the entrance region of a curved jet stream, which is highly subgeostrophic, with the entrance region of a straight jet stream, which is highly supergeostrophic. The wind and mass fields become misphased as the entrance regions converge resulting in the significant spatial variation of inertial forcing, centripetal forcing, and along- and cross-stream pressure gradient forcing over a mesobeta scale region. This results in frontogenesis and the along-stream divergence of cyclonic and convergence of cyclonic ageostrophic vertical vorticity. The centripetally forced mesoscale front becomes the locus of large gradients of ageostrophic vertical vorticity along an overturning isentrope. This region becomes favorable for streamwise vorticity gradient formation enhancing the environment for organization of horizontal vortex tubes in the presence of buoyant forcing.

Demonstration of lysosomal localization for the mammalian ependymin-related protein using classical approaches combined with a novel density shift method.

PubMed

Della Valle, Maria Cecilia; Sleat, David E; Sohar, Istvan; Wen, Ting; Pintar, John E; Jadot, Michel; Lobel, Peter

2006-11-17

Most newly synthesized soluble lysosomal proteins are delivered to the lysosome via the mannose 6-phosphate (Man-6-P)-targeting pathway. The presence of the Man-6-P post-translational modification allows these proteins to be affinity-purified on immobilized Man-6-P receptors. This approach has formed the basis for a number of proteomic studies that identified multiple as yet uncharacterized Man-6-P glycoproteins that may represent new lysosomal proteins. Although the presence of Man-6-P is suggestive of lysosomal function, the subcellular localization of such candidates requires experimental verification. Here, we have investigated one such candidate, ependymin-related protein (EPDR). EPDR is a protein of unknown function with some sequence similarity to ependymin, a fish protein thought to play a role in memory consolidation and learning. Using classical subcellular fractionation on rat brain, EPDR co-distributes with lysosomal proteins, but there is significant overlap between lysosomal and mitochondrial markers. For more definitive localization, we have developed a novel approach based upon a selective buoyant density shift of the brain lysosomes in a mutant mouse lacking NPC2, a lysosomal protein involved in lipid transport. EPDR, in parallel with lysosomal markers, shows this density shift in gradient centrifugation experiments comparing mutant and wild type mice. This approach, combined with morphological analyses, demonstrates that EPDR resides in the lysosome. In addition, the lipidosis-induced density shift approach represents a valuable tool for identification and validation of both luminal and membrane lysosomal proteins that should be applicable to high throughput proteomic studies.

Convective and interfacial instabilities during solidification of succinonitrile containing ethanol

NASA Technical Reports Server (NTRS)

Schaefer, R. J.; Coriell, S. R.

1982-01-01

Even though slow convective flow is difficult to detect in solidifying metals, it can readily be observed in transparent materials by observing the motion of small neutrally buoyant particles. Succinonitrile, which solidifies with an unfaceted solid/liquid interface and has well characterized physical properties, is considered an excellent material for such studies. For studies of solute-induced convection, ethanol is a useful addition to succinonitrile since it has a lower density and a somewhat similar molecular structure. Samples of high purity and ethanol-doped succinonitrile are unidirectionally solidified in a vertical temperature gradient. Latex mimcrospheres 2 microns in diameter are suspended in the liquid to reveal the convective flow. Convective and morphological stability is observed as a function of solute concentration and growth velocity. These measurements are compared with theoretical calculations that predict the transition from stability to instability as a function of solidification conditions. The predicted transitions occur at low concentrations and solidification velocities; for this reason, extreme care must be taken in order to eliminate the effects of impurities or thermally induced convection.

Incorporating Density Properties of MgSO4 Brines Into Icy World Ocean Simulations

NASA Astrophysics Data System (ADS)

Goodman, J. C.; Vance, S.

2011-12-01

The structure and flow of the subsurface oceans in icy worlds depends on the sources of buoyancy within these oceans. Buoyancy is determined by the equation of state, in which density is a nonlinear function of temperature, salinity, and pressure. Equations of state for terrestrial seawater (with Na and Cl as the principal dissolved species) are well-developed, but icy world oceans may contain a different balance of species, including Na, Mg, SO4, and NH4 (Kargel et al, 2000). Recent work by Vance and Brown (2011, pers. comm.) has mapped out the density and thermodynamic properties of MgSO4 brines under icy world conditions. We have developed code to incorporate this equation of state data for MgSO4 brines into two different ocean simulation models. First, we investigate a single-column convection model, which is able to find the equilibrium structure and heat transport of an icy world ocean. We explore the heat transport through the ocean subject to a variety of assumptions about ocean salinity and seafloor heat and salt flux. We resolve the paradox posed by Vance and Brown (2004): warm salty MgSO4 brine emitted by a seafloor hydrothermal system may be positively buoyant at the seafloor, but become negatively buoyant (sinking) at lower pressure. How does heat escape the ocean, if it cannot be transported by convection? Second, we add MgSO4 dynamics to a full 3-D time-dependent general circulation model (the MIT GCM), which is able to simulate both the global-scale circulation of the world's ocean and investigate the highly turbulent dynamics of buoyant hydrothermal systems. We ask, "Are buoyancy-driven flows in a MgSO4 brine ocean significantly different than similarly-driven flows in terrestrial seawater?"

A numerical study of variable density flow and mixing in porous media

NASA Astrophysics Data System (ADS)

Fan, Yin; Kahawita, René

1994-10-01

A numerical study of a negatively buoyant plume intruding into a neutrally stratified porous medium has been undertaken using finite different methods. Of particular interest has been to ascertain whether the experimentally observed gravitational instabilities that form along the lower edge of the plume are reproduced in the numerical model. The model has been found to faithfully reproduce the mean flow as well as the gravitational instabilities in the intruding plume. A linear stability analysis has confirmed the fact that the negatively buoyant plume is in fact gravitationally unstable and that the stability depends on two parameters: a concentration Rayleigh number and a characteristic length scale which is dependent on the transverse dispersivity.

Coupled Thermo-Hydro-Chemical (THC) Modeling of Hypogene Karst Evolution in a Prototype Mountain Hydrologic System

NASA Astrophysics Data System (ADS)

Chaudhuri, A.; Rajaram, H.; Viswanathan, H. S.; Zyvoloski, G.

2011-12-01

Hypogene karst systems are believed to develop when water flowing upward against the geothermal gradient dissolves limestone as it cools. We present a comprehensive THC model incorporating time-evolving fluid flow, heat transfer, buoyancy effects, multi-component reactive transport and aperture/permeability change to investigate the origin of hypogene karst systems. Our model incorporates the temperature and pressure dependence of the solubility and dissolution kinetics of calcite. It also allows for rigorous representation of temperature-dependent fluid density and its influence on buoyancy forces at various stages of karstification. The model is applied to investigate karstification over geological time scales in a prototype mountain hydrologic system. In this system, a high water table maintained by mountain recharge, drives flow downward through the country rock and upward via a high-permeability fault/fracture. The pressure boundary conditions are maintained constant in time. The fluid flux through the fracture remains nearly constant even though the fracture aperture and permeability increase by dissolution, largely because the permeability of the country rock is not altered significantly due to slower dissolution rates. However, karstification by fracture dissolution is not impeded even though the fluid flux stays nearly constant. Forced and buoyant convection effects arise due to the increased permeability of the evolving fracture system. Since in reality the aperture varies significantly within the fracture plane, the initial fracture aperture is modeled as a heterogeneous random field. In such a heterogeneous aperture field, the water initially flows at a significant rate mainly through preferential flow paths connecting the relatively large aperture zones. Dissolution is more prominent at early time along these flow paths, and the aperture grows faster within these paths. With time, the aperture within small sub-regions of these preferential flow paths grows to a point where the permeability is large enough for the onset of buoyant convection. As a result, a multitude of buoyant convection cells form that take on a two-dimensional (2D) maze-like appearance, which could represent a 2D analog of the three-dimensional (3D) mazework pattern widely thought to be characteristic of hypogene cave systems. Although computational limitations limited us to 2D, we suggest that similar process interactions in a 3D network of fractures and faults could produce a 3D mazework.

Experimental investigation of large-scale vortices in a freely spreading gravity current

NASA Astrophysics Data System (ADS)

Yuan, Yeping; Horner-Devine, Alexander R.

2017-10-01

A series of laboratory experiments are presented to compare the dynamics of constant-source buoyant gravity currents propagating into laterally confined (channelized) and unconfined (spreading) environments. The plan-form structure of the spreading current and the vertical density and velocity structures on the interface are quantified using the optical thickness method and a combined particle image velocimetry and planar laser-induced fluorescence method, respectively. With lateral boundaries, the buoyant current thickness is approximately constant and Kelvin-Helmholtz instabilities are generated within the shear layer. The buoyant current structure is significantly different in the spreading case. As the current spreads laterally, nonlinear large-scale vortex structures are observed at the interface, which maintain a coherent shape as they propagate away from the source. These structures are continuously generated near the river mouth, have amplitudes close to the buoyant layer thickness, and propagate offshore at speeds approximately equal to the internal wave speed. The observed depth and propagation speed of the instabilities match well with the fastest growing mode predicted by linear stability analysis, but with a shorter wavelength. The spreading flows have much higher vorticity, which is aggregated within the large-scale structures. Secondary instabilities are generated on the leading edge of the braids between the large-scale vortex structures and ultimately break and mix on the lee side of the structures. Analysis of the vortex dynamics shows that lateral stretching intensifies the vorticity in the spreading currents, contributing to higher vorticity within the large-scale structures in the buoyant plume. The large-scale instabilities and vortex structures observed in the present study provide new insights into the origin of internal frontal structures frequently observed in coastal river plumes.

Simultaneous generation of Superpiles and Superplumes in the lower mantle

NASA Astrophysics Data System (ADS)

Ballmer, M. D.; Lekic, V.; Ito, G.

2014-12-01

Seismic tomography reveals two antipodal large low shear-wave velocity provinces (LLSVP) at the base of the mantle, rising up to ~1900 km above the core-mantle boundary (CMB). A compositional distinction between the LLSVPs and the ambient mantle is supported by anti-correlation of bulk-sound and shear-wave velocity (Vs) anomalies as well as steep lateral gradients in Vs along the edges of the LLSVPs. These seismic observations however are mainly restricted to the bottom ~600 km of the mantle. Mineral-physics constraints on elastic properties of high-pressure rocks suggest that the seismic signature of these deep distinct domains (DDD) is unlikely to be caused by the presence of subducted basalt, but rather by that of primitive mantle. They further suggest that the LLSVP's top domains (that reach from heights of ~600 km to 1900 km above the CMB) are either composed of hot basaltic or warm average-mantle material. From a geodynamical point of view, however, the former explanation appears to be more consistent with the top domain's large widths. Here, we present a series of 2D numerical models of mantle convection with three distinct materials (representative of pyrolite, primitive and basaltic material), exploring the effects of their distinct densities and compressibilities. We find (1) that the dense primitive materials accumulate as Superpiles at the CMB, similar to the DDDs, and (2) that the moderately dense basaltic materials evolve into Superplumes sitting on top of the Superpiles, similar to the top LLSVP domains. We here refer to Superplumes as thermochemical domes that are buoyant at depth but negatively buoyant in the mid-mantle (due to excess heat and relatively low compressibility), where they stagnate. Small plumelets intermittently rise from the roofs of the Superplumes to entrain basalt that has evolved in the lower mantle and form hotspots at the surface. This prediction addresses the geochemical and geochronological record of intraplate Pacific volcanism. The predicted sub-horizontal compositional boundary between the basal Superpiles and the overlying Superplumes further provides an explanation for steep vertical gradients in Vs observed at 400-700 km height above the CMB. Such a LLSVP subdivision holds implications for the early and ongoing differentiation and thermal evolution of our planet.

Extended Subadiabatic Layer in Simulations of Overshooting Convection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Käpylä, Petri J.; Arlt, Rainer; Rheinhardt, Matthias

2017-08-20

We present numerical simulations of hydrodynamic overshooting convection in local Cartesian domains. We find that a substantial fraction of the lower part of the convection zone (CZ) is stably stratified according to the Schwarzschild criterion while the enthalpy flux is outward directed. This occurs when the heat conduction profile at the bottom of the CZ is smoothly varying, based either on a Kramers-like opacity prescription as a function of temperature and density or a static profile of a similar shape. We show that the subadiabatic layer arises due to nonlocal energy transport by buoyantly driven downflows in the upper partsmore » of the CZ. Analysis of the force balance of the upflows and downflows confirms that convection is driven by cooling at the surface. We find that the commonly used prescription for the convective enthalpy flux being proportional to the negative entropy gradient does not hold in the stably stratified layers where the flux is positive. We demonstrate the existence of a non-gradient contribution to the enthalpy flux, which is estimated to be important throughout the convective layer. A quantitative analysis of downflows indicates a transition from a tree-like structure where smaller downdrafts merge into larger ones in the upper parts to a structure in the deeper parts where a height-independent number of strong downdrafts persist. This change of flow topology occurs when a substantial subadiabatic layer is present in the lower part of the CZ.« less

Downregulation of surface sodium pumps by endocytosis during meiotic maturation of Xenopus laevis oocytes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmalzing, G.; Eckard, P.; Kroener, S.P.

1990-01-01

During meiotic maturation, plasma membranes of Xenopus laevis oocytes completely lose the capacity to transport Na and K and to bind ouabain. To explore whether the downregulation might be due to an internalization of the sodium pump molecules, the intracellular binding of ouabain was determined. Selective permeabilization of the plasma membrane of mature oocytes (eggs) by digitonin almost failed to disclose ouabain binding sites. However, when the eggs were additionally treated with 0.02% sodium dodecyl sulfate (SDS) to permeabilize inner membranes, all sodium pumps present before maturation were recovered. Phosphorylation by (gamma-32P)ATP combined with SDS-polyacrylamide gel electrophoresis (PAGE) and autoradiographymore » showed that sodium pumps were greatly reduced in isolated plasma membranes of eggs. According to sucrose gradient fractionation, maturation induced a shift of sodium pumps from the plasma membrane fraction to membranes of lower buoyant density with a protein composition different from that of the plasma membrane. Endocytosed sodium pumps identified on the sucrose gradient from (3H)ouabain bound to the cell surface before maturation could be phosphorylated with inorganic (32P)phosphate. The findings suggest that downregulation of sodium pumps during maturation is brought about by translocation of surface sodium pumps to an intracellular compartment, presumably endosomes. This contrasts the mechanism of downregulation of Na-dependent cotransport systems, the activities of which are reduced as a consequence of a maturation-induced depolarization of the membrane without a removal of the corresponding transporter from the plasma membrane.« less

Membrane-associated mucins in normal human conjunctiva.

PubMed

Berry, M; Ellingham, R B; Corfield, A P

2000-02-01

To examine the presence of specific membrane-associated mucins in normal human conjunctiva. Glycoconjugates were extracted from membranes with two detergents: octylglucoside and Triton X114. Mucins were separated by cesium chloride density gradient centrifugation. Size was assessed by gel filtration on Sepharose CL2B and charge by ion-exchange chromatography on MonoQ. Cross reaction with antibodies against mucin gene products was assessed in blots of electrophoresis gels. Extraction of total tissue membranes yielded material with a buoyant density typical of mucins. Gel filtration showed material reacting with antimucin antibodies in a range of molecular sizes. Agarose electrophoresis confirmed the presence of MUC1 and MUC4 and the absence of MUC2 or MUC5AC. Isolation of membrane mucins by sequential, exhaustive extraction with octylglucoside followed by Triton X114 suggested the existence of mucins in different membrane environments. Reagents to carbohydrate epitopes revealed high mobility material, comigrating with MUC1 and MUC4. Low mobility membrane-bound mucins did not cross-react with any antibodies to mucin genes known to be expressed in human conjunctiva. Membrane-associated mucins are distinct from secreted mucins in normal human conjunctiva. MUC1 and MUC4 mature products decorate the membranes of conjunctival epithelial cells. Their segregation between octyl glucoside and the detergent and aqueous phases of Triton X114 suggests a variety of membrane anchoring modes.

Sedimentation equilibrium and the generalized Archimedes' principle.

PubMed

Parola, Alberto; Buzzaccaro, Stefano; Secchi, Eleonora; Piazza, Roberto

2013-03-21

The buoyancy concept is critically re-examined for applications to dispersions of nano-particles, such as colloids, proteins, or macromolecules. It is shown that when the size of the buoyant particle is not too different (say, at most a factor of ten) from the size of the dispersed particles, new intriguing phenomena emerge, leading to the violation of the Archimedes' principle. The resulting buoyancy force depends not only on the volume of the particle and on the mass density of the dispersion, but also on the relative size of the particles, on their geometry, and on the interactions between the buoyant particle and the fluid. Explicit expressions for such a generalized Archimedes' principle are obtained and the results are tested against targeted experiments in colloidal dispersions.

Sedimentation equilibrium and the generalized Archimedes' principle

NASA Astrophysics Data System (ADS)

Parola, Alberto; Buzzaccaro, Stefano; Secchi, Eleonora; Piazza, Roberto

2013-03-01

The buoyancy concept is critically re-examined for applications to dispersions of nano-particles, such as colloids, proteins, or macromolecules. It is shown that when the size of the buoyant particle is not too different (say, at most a factor of ten) from the size of the dispersed particles, new intriguing phenomena emerge, leading to the violation of the Archimedes' principle. The resulting buoyancy force depends not only on the volume of the particle and on the mass density of the dispersion, but also on the relative size of the particles, on their geometry, and on the interactions between the buoyant particle and the fluid. Explicit expressions for such a generalized Archimedes' principle are obtained and the results are tested against targeted experiments in colloidal dispersions.

High-Aspect-Ratio Rotating Cell-Culture Vessel

NASA Technical Reports Server (NTRS)

Wolf, David A.; Sams, Clarence; Schwarz, Ray P.

1992-01-01

Cylindrical rotating cell-culture vessel with thin culture-medium layer of large surface area provides exchange of nutrients and products of metabolism with minimal agitation. Rotation causes averaging of buoyant forces otherwise separating components of different densities. Vessel enables growth of cells in homogeneous distribution with little agitation and little shear stress.

On the response of superpressure balloons to displacements from equilibrium density level

NASA Technical Reports Server (NTRS)

Levanon, N.; Kushnir, Y.

1976-01-01

The response of a superpressure balloon to an initial displacement from its constant-density floating level is examined. An approximate solution is obtained to the governing vertical equation of motion for constant-density superpressure balloons. This solution is used to filter out neutrally buoyant oscillations in balloon records despite the nonlinear behavior of the balloon. The graph depicting the pressure data after deconvolution between the raw pressure data and the normalized balloon wavelet shows clearly the strong filtering-out of the neutral buoyancy oscillations.

Gravitational Effects on Flow Instability and Transition in Low Density Jets

NASA Technical Reports Server (NTRS)

Agrawal A. K.; Parthasarathy, K.; Pasumarthi, K.; Griffin, D. W.

2000-01-01

Recent experiments have shown that low-density gas jets injected into a high-density ambient gas undergo an instability mode, leading to highly-periodic oscillations in the flow-field for certain conditions. The transition from laminar to turbulent flow in these jets is abrupt, without the gradual change in scales. Even the fine scale turbulent structure repeats itself with extreme regularity from cycle to cycle. Similar observations were obtained in buoyancy-dominated and momentum-dominated jets characterized by the Richardson numbers, Ri = [gD(rho(sub a)-rho(sub j))/rho(sub j)U(sub j)(exp 2) ] where g is the gravitational acceleration, D is the jet diameter, rho(sub a) and rho(sub a) are, respectively, the free-stream and jet densities, and U(sub j) is the mean jet exit velocity. At high Richardson numbers, the instability is presumably caused by buoyancy since the flow-oscillation frequency (f) or the Strouhal number, St = [fD/U(sub j)] scales with Ri. In momentum-dominated jets, however, the Strouhal number of the oscillating flow is relatively independent of the Ri. In this case, a local absolute instability is predicted in the potential core of low-density jets with S [= rho(sub j)/rho(sub a)] < 0.7, which agrees qualitatively with experiments. Although the instability in gas jets of high Richardson numbers is attributed to buoyancy, direct physical evidence has not been acquired in experiments. If the instability is indeed caused by buoyancy, the near-field flow structure of the jet will change significantly when the buoyancy is removed, for example, in the microgravity environment. Thus, quantitative data on the spatial and temporal evolutions of the instability, length and time scale of the oscillating mode and its effects on the mean flow and breakdown of the potential core are needed in normal and microgravity to delineate gravitational effects in buoyant jets. In momentum dominated low-density jets, the instability is speculated to originate in the potential core. However, experiments have not succeeded in identifying the direct physical cause of the instability. For example, the theory predicts an oscillating mode for S<0.62 in the limit of zero momentum thickness, which contradicts with the experimental findings of Kyle and Sreenivasan. The analyses of momentum-dominated jets neglect buoyancy effects because of the small Richardson number. Although this assumption is appropriate in the potential core, the gravitational effects are important in the annular region surrounding the jet, where the density and velocity gradients are large. This reasoning provides basis for the hypothesis that the instability in low Richardosn number jets studied by Kyle and Sreenivasan and Monkewitz et al. is caused by buoyancy. The striking similarity in characteristics of the instability and virtually the identical conclusions reached by Subbarao and Cantwell in buoyant (Ri>0.5) helium jets on one hand and by Kyle and Sreenivasan in momentum-dominated (Ri<1x10(exp -3)) helium jets on the other support this hypothesis. However, quantitative experiments in normal and microgravity are necessary to obtain direct physical evidence of buoyancy effects on the flow instability and structure of momentum-dominated low-density jets. The primary objective of this new research project is to quantify how buoyancy affects the flow instability and structure in the near field of low-density jets. The flow will be described by the spatial and temporal evolutions of the instability, length and time scales of the oscillating mode, and the mean and fluctuating concentration fields. To meet this objective, concentration measurements will be obtained across the whole field using quantitative Rainbow Schlieren Deflectometry, providing spatial resolution of 0.1mm and temporal resolution of 0.017s to 1ms. The experimental effort will be supplemented with linear stability analysis of low-density jets by considering buoyancy. The first objective of this research is to investigate the effects of gravity on the flow instability and structure of low-density jets. The flow instability in these jets has been attributed to buoyancy. By removing buoyancy in our experiments, we seek to obtain the direct physical evidence of the instability mechanism. In the absence of the instability, the flow structure will undergo a significant change. We seek to quantify these changes by mapping the flow field (in terms of the concentration profiles) of these jets at non-buoyant conditions. Such information is presently lacking in the existing literature. The second objective of this research is to determine if the instability in momentum-driven, low-density jets is caused by buoyancy. At these conditions, the buoyancy effects are commonly ignored because of the small Richardson based on global parameters. By eliminating buoyancy in our experiments, globally as well as locally, we seek to examine the possibility that the instability mechanism in self-excited, buoyant or momentum-driven jets is the same. To meet this objective, we would quantify the jet flow in normal and microgravity, while systematically decreasing the Richardson number from buoyancy-driven to momentum driven flow regime. The third objective of this research is to perform a linear stability analysis of low-density gas jets by including the gravitational effects. The flow oscillations in these jets are attributed to an absolute instability, whereby the disturbance grows exponentially at the site to ultimately contaminate the entire flow field. We seek to study the characteristics of both convective and absolute instabilities and demarcate the boundary between them.

Reconciling Long-Wavelength Dynamic Topography, Geoid Anomalies and Mass Distribution on Earth

NASA Astrophysics Data System (ADS)

Hoggard, M.; Richards, F. D.; Ghelichkhan, S.; Austermann, J.; White, N.

2017-12-01

Since the first satellite observations in the late 1950s, we have known that that the Earth's non-hydrostatic geoid is dominated by spherical harmonic degree 2 (wavelengths of 16,000 km). Peak amplitudes are approximately ± 100 m, with highs centred on the Pacific Ocean and Africa, encircled by lows in the vicinity of the Pacific Ring of Fire and at the poles. Initial seismic tomography models revealed that the shear-wave velocity, and therefore presumably the density structure, of the lower mantle is also dominated by degree 2. Anti-correlation of slow, probably low density regions beneath geoid highs indicates that the mantle is affected by large-scale flow. Thus, buoyant features are rising and exert viscous normal stresses that act to deflect the surface and core-mantle boundary (CMB). Pioneering studies in the 1980s showed that a viscosity jump between the upper and lower mantle is required to reconcile these geoid and tomographically inferred density anomalies. These studies also predict 1-2 km of dynamic topography at the surface, dominated by degree 2. In contrast to this prediction, a global observational database of oceanic residual depth measurements indicates that degree 2 dynamic topography has peak amplitudes of only 500 m. Here, we attempt to reconcile observations of dynamic topography, geoid, gravity anomalies and CMB topography using instantaneous flow kernels. We exploit a density structure constructed from blended seismic tomography models, combining deep mantle imaging with higher resolution upper mantle features. Radial viscosity structure is discretised, and we invert for the best-fitting viscosity profile using a conjugate gradient search algorithm, subject to damping. Our results suggest that, due to strong sensitivity to radial viscosity structure, the Earth's geoid seems to be compatible with only ± 500 m of degree 2 dynamic topography.

Integrated Monitoring of Mola mola Behaviour in Space and Time.

PubMed

Sousa, Lara L; López-Castejón, Francisco; Gilabert, Javier; Relvas, Paulo; Couto, Ana; Queiroz, Nuno; Caldas, Renato; Dias, Paulo Sousa; Dias, Hugo; Faria, Margarida; Ferreira, Filipe; Ferreira, António Sérgio; Fortuna, João; Gomes, Ricardo Joel; Loureiro, Bruno; Martins, Ricardo; Madureira, Luis; Neiva, Jorge; Oliveira, Marina; Pereira, João; Pinto, José; Py, Frederic; Queirós, Hugo; Silva, Daniel; Sujit, P B; Zolich, Artur; Johansen, Tor Arne; de Sousa, João Borges; Rajan, Kanna

2016-01-01

Over the last decade, ocean sunfish movements have been monitored worldwide using various satellite tracking methods. This study reports the near-real time monitoring of fine-scale (< 10 m) behaviour of sunfish. The study was conducted in southern Portugal in May 2014 and involved satellite tags and underwater and surface robotic vehicles to measure both the movements and the contextual environment of the fish. A total of four individuals were tracked using custom-made GPS satellite tags providing geolocation estimates of fine-scale resolution. These accurate positions further informed sunfish areas of restricted search (ARS), which were directly correlated to steep thermal frontal zones. Simultaneously, and for two different occasions, an Autonomous Underwater Vehicle (AUV) video-recorded the path of the tracked fish and detected buoyant particles in the water column. Importantly, the densities of these particles were also directly correlated to steep thermal gradients. Thus, both sunfish foraging behaviour (ARS) and possibly prey densities, were found to be influenced by analogous environmental conditions. In addition, the dynamic structure of the water transited by the tracked individuals was described by a Lagrangian modelling approach. The model informed the distribution of zooplankton in the region, both horizontally and in the water column, and the resultant simulated densities positively correlated with sunfish ARS behaviour estimator (rs = 0.184, p<0.001). The model also revealed that tracked fish opportunistically displace with respect to subsurface current flow. Thus, we show how physical forcing and current structure provide a rationale for a predator's fine-scale behaviour observed over a two weeks in May 2014.

Integrated Monitoring of Mola mola Behaviour in Space and Time

PubMed Central

Sousa, Lara L.; López-Castejón, Francisco; Gilabert, Javier; Relvas, Paulo; Couto, Ana; Queiroz, Nuno; Caldas, Renato; Dias, Paulo Sousa; Dias, Hugo; Faria, Margarida; Ferreira, Filipe; Ferreira, António Sérgio; Fortuna, João; Gomes, Ricardo Joel; Loureiro, Bruno; Martins, Ricardo; Madureira, Luis; Neiva, Jorge; Oliveira, Marina; Pereira, João; Pinto, José; Py, Frederic; Queirós, Hugo; Silva, Daniel; Sujit, P. B.; Zolich, Artur; Johansen, Tor Arne; de Sousa, João Borges; Rajan, Kanna

2016-01-01

Over the last decade, ocean sunfish movements have been monitored worldwide using various satellite tracking methods. This study reports the near-real time monitoring of fine-scale (< 10 m) behaviour of sunfish. The study was conducted in southern Portugal in May 2014 and involved satellite tags and underwater and surface robotic vehicles to measure both the movements and the contextual environment of the fish. A total of four individuals were tracked using custom-made GPS satellite tags providing geolocation estimates of fine-scale resolution. These accurate positions further informed sunfish areas of restricted search (ARS), which were directly correlated to steep thermal frontal zones. Simultaneously, and for two different occasions, an Autonomous Underwater Vehicle (AUV) video-recorded the path of the tracked fish and detected buoyant particles in the water column. Importantly, the densities of these particles were also directly correlated to steep thermal gradients. Thus, both sunfish foraging behaviour (ARS) and possibly prey densities, were found to be influenced by analogous environmental conditions. In addition, the dynamic structure of the water transited by the tracked individuals was described by a Lagrangian modelling approach. The model informed the distribution of zooplankton in the region, both horizontally and in the water column, and the resultant simulated densities positively correlated with sunfish ARS behaviour estimator (rs = 0.184, p<0.001). The model also revealed that tracked fish opportunistically displace with respect to subsurface current flow. Thus, we show how physical forcing and current structure provide a rationale for a predator’s fine-scale behaviour observed over a two weeks in May 2014. PMID:27494028

The initial giant umbrella cloud of the May 18th, 1980, explosive eruption of Mount St. Helens

USGS Publications Warehouse

Sparks, R.S.J.; Moore, J.G.; Rice, C.J.

1986-01-01

The initial eruption column of May 18th, 1980 reached nearly 30 km altitude and released 1017 joules of thermal energy into the atmosphere in only a few minutes. Ascent of the cloud resulted in forced intrusion of a giant umbrella-shaped cloud between altitudes of 10 and 20 km at radial horizontal velocities initially in excess of 50 m/s. The mushroom cloud expanded 15 km upwind, forming a stagnation point where the radial expansion velocity and wind velocity were equal. The cloud was initiated when the pyroclastic blast flow became buoyant. The flow reduced its density as it moved away from the volcano by decompression, by sedimentation, and by mixing with and heating the surrounding air. Observations indicate that much of the flow, covering an area of 600 km2, became buoyant within 1.5 minutes and abruptly ascended to form the giant cloud. Calculations are presented for the amount of air that must have been entrained into the flow to make it buoyant. Assuming an initial temperature of 450??C and a magmatic origin for the explosion, these calculations indicate that the flow became buoyant when its temperature was approximately 150??C and the flow consisted of a mixture of 3.25 ?? 1011 kg of pyroclasts and 5.0 ?? 1011 kg of air. If sedimentation is considered, these figures reduce to 1.1 ?? 1011 kg of pyroclasts and 1.0 ?? 1011 kg of air. ?? 1986.

Eddy diffusivity of quasi-neutrally-buoyant inertial particles

NASA Astrophysics Data System (ADS)

Martins Afonso, Marco; Muratore-Ginanneschi, Paolo; Gama, Sílvio M. A.; Mazzino, Andrea

2018-04-01

We investigate the large-scale transport properties of quasi-neutrally-buoyant inertial particles carried by incompressible zero-mean periodic or steady ergodic flows. We show how to compute large-scale indicators such as the inertial-particle terminal velocity and eddy diffusivity from first principles in a perturbative expansion around the limit of added-mass factor close to unity. Physically, this limit corresponds to the case where the mass density of the particles is constant and close in value to the mass density of the fluid, which is also constant. Our approach differs from the usual over-damped expansion inasmuch as we do not assume a separation of time scales between thermalization and small-scale convection effects. For a general flow in the class of incompressible zero-mean periodic velocity fields, we derive closed-form cell equations for the auxiliary quantities determining the terminal velocity and effective diffusivity. In the special case of parallel flows these equations admit explicit analytic solution. We use parallel flows to show that our approach sheds light onto the behavior of terminal velocity and effective diffusivity for Stokes numbers of the order of unity.

Nucleoprotein Complexes Containing Replicating Simian Virus 40 DNA: Comparison with Polyoma Nucleoprotein Complexes

PubMed Central

Hall, Mark R.; Meinke, William; Goldstein, David A.

1973-01-01

Procedures for isolating nucleoprotein complexes containing replicating polyoma DNA from infected mouse cells were used to prepare short-lived nucleoprotein complexes (r-SV40 complexes) containing replicating simian virus 40 (SV40) DNA from infected monkey cells. Like the polyoma complexes, r-SV40 complexes were only partially released from nuclei by cell lysis but could be extracted from nuclei by prolonged treatment with solutions containing Triton X-100. r-SV40 complexes sedimented faster than complexes containing SV40 supercoiled DNA (SV40 complex) in sucrose gradients, and both types of SV40 nucleoprotein complexes sedimented ahead of polyoma complexes containing supercoiled polyoma DNA (py complex). The sedimentation rates of py complex and SV40 complex were 56 and 61S, respectively, based on the sedimentation rate of the mouse large ribosomal subunit as a marker. r-SV40 complexes sedimented as multiple peaks between 56 and 75S. Sedimentation and buoyant density measurements indicated that protein is bound to all forms of SV40 DNA at about the same ratio of protein to DNA (1-2/1) as was reported for polyoma nucleoproteins. PMID:4359958

Trench Advance By the Subduction of Buoyant Features - Application to the Izu-Bonin-Marianas Arc

NASA Astrophysics Data System (ADS)

Goes, S. D. B.; Fourel, L.; Morra, G.

2014-12-01

Most subduction trenches retreat, not only today but throughout the Cenozoic. However, a few trenches clearly advance during part of the evolution, including Izu-Bonin Marianas (IBM) and Kermadec. Trench retreat is well understood as a basic consequence of slab pull, but it is debated what causes trench advance. The IBM trench underwent a complex evolution: right after its initiation, it rotated clockwise, leading to very fast retreat in the north and slow retreat in the south. But since 10-15 Ma, IBM trench motions have switched to advance at the southern end, and since 5 Ma also the northern end is advancing. Based on 2-D subduction models, it has been proposed proposed that the change in age of the subducting plate at the IBM trench (from 40-70 m.y. at the initiation of the trench 45 m.y. ago to 100-140 m.y. lithosphere subducting at the trench today) and its effect on plate strength could explain the transition from trench retreat to trench advance, and that the age gradient (younger in the north and older in the south) could explain the rotation of the trench. However, with new 3-D coupled fluid-solid subduction model where we can include such lateral age gradients, we find that this does not yield the observed behaviour. Instead, we propose an alternative mechanism, involving the subduction of the buoyant Caroline Island Ridge at the southern edge of the Mariana trench and show that it can explain both trench motion history and the current morphology of the IBM slab as imaged by seismic tomography.

Combining Step Gradients and Linear Gradients in Density.

PubMed

Kumar, Ashok A; Walz, Jenna A; Gonidec, Mathieu; Mace, Charles R; Whitesides, George M

2015-06-16

Combining aqueous multiphase systems (AMPS) and magnetic levitation (MagLev) provides a method to produce hybrid gradients in apparent density. AMPS—solutions of different polymers, salts, or surfactants that spontaneously separate into immiscible but predominantly aqueous phases—offer thermodynamically stable steps in density that can be tuned by the concentration of solutes. MagLev—the levitation of diamagnetic objects in a paramagnetic fluid within a magnetic field gradient—can be arranged to provide a near-linear gradient in effective density where the height of a levitating object above the surface of the magnet corresponds to its density; the strength of the gradient in effective density can be tuned by the choice of paramagnetic salt and its concentrations and by the strength and gradient in the magnetic field. Including paramagnetic salts (e.g., MnSO4 or MnCl2) in AMPS, and placing them in a magnetic field gradient, enables their use as media for MagLev. The potential to create large steps in density with AMPS allows separations of objects across a range of densities. The gradients produced by MagLev provide resolution over a continuous range of densities. By combining these approaches, mixtures of objects with large differences in density can be separated and analyzed simultaneously. Using MagLev to add an effective gradient in density also enables tuning the range of densities captured at an interface of an AMPS by simply changing the position of the container in the magnetic field. Further, by creating AMPS in which phases have different concentrations of paramagnetic ions, the phases can provide different resolutions in density. These results suggest that combining steps in density with gradients in density can enable new classes of separations based on density.

A rapid method for the preparation of ultrapure, functional lysosomes using functionalized superparamagnetic iron oxide nanoparticles.

PubMed

Walker, Mathew W; Lloyd-Evans, Emyr

2015-01-01

Lysosomes are an emerging and increasingly important cellular organelle. With every passing year, more novel proteins and key cellular functions are associated with lysosomes. Despite this, the methodologies for their purification have largely remained unchanged since the days of their discovery. With little advancement in this area, it is no surprise that analysis of lysosomal function has been somewhat stymied, largely in part by the change in buoyant densities that occur under conditions where lysosomes accumulate macromolecules. Such phenotypes are often associated with the lysosomal storage diseases but are increasingly being observed under conditions where lysosomal proteins or, in some cases, cellular functions associated with lysosomal proteins are being manipulated. These altered lysosomes poise a problem to the classical methods to purify lysosomes that are reliant largely on their correct sedimentation by density gradient centrifugation. Building upon a technique developed by others to purify lysosomes magnetically, we have developed a unique assay using superparamagnetic iron oxide nanoparticles (SPIONs) to purify high yields of ultrapure functional lysosomes from multiple cell types including the lysosomal storage disorders. Here we describe this method in detail, including the rationale behind using SPIONs, the potential pitfalls that can be avoided and the potential functional assays these lysosomes can be used for. Finally we also summarize the other methodologies and the exact reasons why magnetic purification of lysosomes is now the method of choice for lysosomal researchers. Copyright © 2015 Elsevier Inc. All rights reserved.

Double-diffusive boundary layers along vertical free surfaces

NASA Astrophysics Data System (ADS)

Napolitano, L. G.; Viviani, A.; Savino, R.

1992-05-01

This paper deals with double-diffusive (or thermosolutal) combined free convection, i.e., free convection due to buoyant forces (natural convection) and surface tension gradients (Marangoni convection), which are generated by volume differences and surface gradients of temperature and solute concentration. Attention is focused on boundary layers that form along a vertical liquid-gas interface, when the appropriately defined nondimensional characteristic transport numbers are large enough, in problems of thermosolutal natural and Marangoni convection, such as buoyancy and surface tension driven flows in differentially heated open cavities and liquid bridges. Classes of similar solutions are derived for each class of convection on the basis of a rigorous order of magnitude analysis. Velocity, temperature and concentration profiles are reported in the similarity plane; flow and transport properties at the liquid-gas interface (interfacial velocity, heat and mass transfer bulk coefficients) are obtained for a wide range of Prandtl and Schmidt numbers and different values of the similarity parameter.

Mapping the Influence of Prior Tectonism on Seismicity in the Central and Eastern US

NASA Astrophysics Data System (ADS)

Boyd, O. S.; Levandowski, W.; Ramirez-Guzman, L.; Zellman, M.; Briggs, R.

2015-12-01

From the Atlantic margin to the Rockies, most earthquakes in the central and eastern U.S. occur in ancient tectonic zones, yet many such features have been historically quiescent. If all intraplate stress were transferred from plate boundaries or bases, the stress field would be broadly uniform, with all well-oriented faults equally likely to slip. But faults are not the only product of tectonism; intrusions, metamorphism, or any number of other alterations may modify crustal and/or upper mantle density, leaving behind lithostatic pressure gradients that can locally elevate or reduce stress on faults. With data provided by Earthscope, we are working to map lithospheric density across the U.S. and to quantify gravitational body-forces using analytical and finite-element methods. Regional-scale 3D models show that gravitational forces focus seismicity and reorient principal stress both in the New Madrid seismic zone and the western Great Plains. Sedimentary fill and low elevation encourage Reelfoot Rift-normal contraction, yet along-strike variations in lower crustal density rotate body-forces beneath New Madrid to interfere constructively with far-field compression, augmenting differential stress by 5-10 MPa. On the plains of SE Colorado and SE Wyoming, the Cheraw and Wheatland/Whalen faults collocate with multiply reactivated Proterozoic sutures, enigmatic Quaternary extension, and focused seismicity with regionally anomalous NW-SE moment tensor T-axes. Earthscope data help reveal anomalously buoyant lower crust beneath each suture -­- which we hypothesize reflects hydration by Farallon slab-derived fluids that have preferentially migrated along ancient fracture networks -- that generates 10 MPa of localized suture-normal tension, consistent with geomorphic strain- and seismic stress-indicators. As continent-wide seismic models emerge from Earthscope data, we will continue to map regions where inherited structures encourage intraplate seismicity.

The dynamics of oceanic fronts. Part 1: The Gulf Stream

NASA Technical Reports Server (NTRS)

Kao, T. W.

1970-01-01

The establishment and maintenance of the mean hydrographic properties of large scale density fronts in the upper ocean is considered. The dynamics is studied by posing an initial value problem starting with a near surface discharge of buoyant water with a prescribed density deficit into an ambient stationary fluid of uniform density. The full time dependent diffusion and Navier-Stokes equations for a constant Coriolis parameter are used in this study. Scaling analysis reveals three independent length scales of the problem, namely a radius of deformation or inertial length scale, Lo, a buoyance length scale, ho, and a diffusive length scale, hv. Two basic dimensionless parameters are then formed from these length scales, the thermal (or more precisely, the densimetric) Rossby number, Ro = Lo/ho and the Ekman number, E = hv/ho. The governing equations are then suitably scaled and the resulting normalized equations are shown to depend on E alone for problems of oceanic interest. Under this scaling, the solutions are similar for all Ro. It is also shown that 1/Ro is a measure of the frontal slope. The governing equations are solved numerically and the scaling analysis is confirmed. The solution indicates that an equilibrium state is established. The front can then be rendered stationary by a barotropic current from a larger scale along-front pressure gradient. In that quasisteady state, and for small values of E, the main thermocline and the inclined isopycnics forming the front have evolved, together with the along-front jet. Conservation of potential vorticity is also obtained in the light water pool. The surface jet exhibits anticyclonic shear in the light water pool and cyclonic shear across the front.

Ocean acidification induces biochemical and morphological changes in the calcification process of large benthic foraminifera.

PubMed

Prazeres, Martina; Uthicke, Sven; Pandolfi, John M

2015-03-22

Large benthic foraminifera are significant contributors to sediment formation on coral reefs, yet they are vulnerable to ocean acidification. Here, we assessed the biochemical and morphological impacts of acidification on the calcification of Amphistegina lessonii and Marginopora vertebralis exposed to different pH conditions. We measured growth rates (surface area and buoyant weight) and Ca-ATPase and Mg-ATPase activities and calculated shell density using micro-computer tomography images. In A. lessonii, we detected a significant decrease in buoyant weight, a reduction in the density of inner skeletal chambers, and an increase of Ca-ATPase and Mg-ATPase activities at pH 7.6 when compared with ambient conditions of pH 8.1. By contrast, M. vertebralis showed an inhibition in Mg-ATPase activity under lowered pH, with growth rate and skeletal density remaining constant. While M. vertebralis is considered to be more sensitive than A. lessonii owing to its high-Mg-calcite skeleton, it appears to be less affected by changes in pH, based on the parameters assessed in this study. We suggest difference in biochemical pathways of calcification as the main factor influencing response to changes in pH levels, and that A. lessonii and M. vertebralis have the ability to regulate biochemical functions to cope with short-term increases in acidity. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

EXPERIMENTS ON BUOYANT PLUME DISPERSION IN A LABORATORY CONVENTION TANK

EPA Science Inventory

Buoyant plume dispersion in the convective boundary layer (CBL) is investigated experimentally in a laboratory convection tank. The focus is on highly-buoyant plumes that loft near the CBL capping inversion and resist downward mixing. Highly- buoyant plumes are those with dimen...

Southern hemisphere craton modification by plume-lithosphere interaction

NASA Astrophysics Data System (ADS)

Hu, J.; Liu, L.; Faccenda, M.; Zhou, Q.; Fischer, K. M.; Marshak, S.; Lundstrom, C.

2017-12-01

The longevity of cratons is generally attributed to neutrally-to-positively buoyant and mechanically strong lithosphere that shields the cratonic crust from underlying mantle dynamics. Large portions of the cratonic lithospheres in South America and Africa, however, have experienced significant modification since the Mesozoic, as demonstrated by widespread Cretaceous uplift and volcanism, present-day high topography, thin crust, and the presence of seismically fast but neutrally buoyant upper-mantle anomalies. We show that these observations reflect a permanent increase in lithospheric buoyancy due to plume-triggered lithosphere deformation and deep lithospheric loss during Late Cretaceous to early Tertiary, as further evidenced by positive lithosphere residual topography, negative lithosphere residual gravity and the realignment of seismic anisotropy in the cratonic roots. Lithosphere in these regions has been thermally reestablished since then, as confirmed by its present-day low heat flow and high seismic velocities. We conclude that lowermost cratonic lithospheres is compositionally denser than the asthenospheric mantle and can be episodically removed when perturbed by underlying mantle dynamics, while the shallower buoyant lithosphere helps to stabilize cratonic crust over billions of years. We further propose that zones where lithosphere was lost would take tens of millions of years to recover thermally, but the density of the new thermal root would remain less than that of the intact root.

Stabilization of electron-scale turbulence by electron density gradient in national spherical torus experiment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruiz Ruiz, J.; White, A. E.; Ren, Y.

2015-12-15

Theory and experiments have shown that electron temperature gradient (ETG) turbulence on the electron gyro-scale, k{sub ⊥}ρ{sub e} ≲ 1, can be responsible for anomalous electron thermal transport in NSTX. Electron scale (high-k) turbulence is diagnosed in NSTX with a high-k microwave scattering system [D. R. Smith et al., Rev. Sci. Instrum. 79, 123501 (2008)]. Here we report on stabilization effects of the electron density gradient on electron-scale density fluctuations in a set of neutral beam injection heated H-mode plasmas. We found that the absence of high-k density fluctuations from measurements is correlated with large equilibrium density gradient, which ismore » shown to be consistent with linear stabilization of ETG modes due to the density gradient using the analytical ETG linear threshold in F. Jenko et al. [Phys. Plasmas 8, 4096 (2001)] and linear gyrokinetic simulations with GS2 [M. Kotschenreuther et al., Comput. Phys. Commun. 88, 128 (1995)]. We also found that the observed power of electron-scale turbulence (when it exists) is anti-correlated with the equilibrium density gradient, suggesting density gradient as a nonlinear stabilizing mechanism. Higher density gradients give rise to lower values of the plasma frame frequency, calculated based on the Doppler shift of the measured density fluctuations. Linear gyrokinetic simulations show that higher values of the electron density gradient reduce the value of the real frequency, in agreement with experimental observation. Nonlinear electron-scale gyrokinetic simulations show that high electron density gradient reduces electron heat flux and stiffness, and increases the ETG nonlinear threshold, consistent with experimental observations.« less

46 CFR 131.865 - Inflatable liferafts and inflatable buoyant apparatus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Inflatable liferafts and inflatable buoyant apparatus... SUPPLY VESSELS OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.865 Inflatable liferafts and inflatable buoyant apparatus. The number of the inflatable liferaft or inflatable buoyant...

46 CFR 131.865 - Inflatable liferafts and inflatable buoyant apparatus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Inflatable liferafts and inflatable buoyant apparatus... SUPPLY VESSELS OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.865 Inflatable liferafts and inflatable buoyant apparatus. The number of the inflatable liferaft or inflatable buoyant...

On the early fate of hydrothermal iron at deep-sea vents: A reassessment after in situ filtration

NASA Astrophysics Data System (ADS)

Waeles, M.; Cotte, L.; Pernet-Coudrier, B.; Chavagnac, V.; Cathalot, C.; Leleu, T.; Laës-Huon, A.; Perhirin, A.; Riso, R. D.; Sarradin, P.-M.

2017-05-01

Deep-sea hydrothermal venting is now recognized as a major source of iron (Fe), an essential trace element that controls marine productivity. However, the reactions occurring during dispersal from buoyant plumes to neutrally buoyant hydrothermal plumes are still poorly constrained. Here we report for the first time on the dissolved-particulate partition of Fe after in situ filtration at the early stage of mixing at different hydrothermal discharges, i.e., Lucky Strike (37°N), TAG (26°N), and Snakepit (23°N) on the Mid-Atlantic Ridge. We found that hydrothermal iron is almost completely preserved (>90%) in the dissolved fraction, arguing for low iron-bearing sulfide precipitation of iron in basalt-hosted systems with low Fe:H2S ratios. This result can only be explained by a kinetically limited formation of pyrite. The small part of Fe being precipitated as sulfides in the mixing gradient (<10%) is restricted to the inclusion of Fe in minerals of high Cu and Zn content. We also show that secondary venting is a source of Fe-depleted hydrothermal solutions. These results provide new constrains on Fe fluxes from hydrothermal venting.

Density Gradients in Chemistry Teaching

ERIC Educational Resources Information Center

Miller, P. J.

1972-01-01

Outlines experiments in which a density gradient might be used to advantage. A density gradient consists of a column of liquid, the composition and density of which varies along its length. The procedure can be used in analysis of solutions and mixtures and in density measures of solids. (Author/TS)

46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

Code of Federal Regulations, 2011 CFR

2011-10-01

... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST... Inspections § 131.580 Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced...

46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

Code of Federal Regulations, 2010 CFR

2010-10-01

... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST... Inspections § 131.580 Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced...

Variation in body condition during the post-moult foraging trip of southern elephant seals and its consequences on diving behaviour.

PubMed

Richard, Gaëtan; Vacquié-Garcia, Jade; Jouma'a, Joffrey; Picard, Baptiste; Génin, Alexandre; Arnould, John P Y; Bailleul, Frédéric; Guinet, Christophe

2014-07-15

Mature female southern elephant seals (Mirounga leonina) come ashore only in October to breed and in January to moult, spending the rest of the year foraging at sea. Mature females may lose as much as 50% of their body mass, mostly in lipid stores, during the breeding season due to fasting and lactation. When departing to sea, post-breeding females are negatively buoyant, and the relative change in body condition (i.e. density) during the foraging trip has previously been assessed by monitoring the descent rate during drift dives. However, relatively few drift dives are performed, resulting in low resolution of the temporal reconstruction of body condition change. In this study, six post-breeding females were equipped with time-depth recorders and accelerometers to investigate whether changes in active swimming effort and speed could be used as an alternative method of monitoring density variations throughout the foraging trip. In addition, we assessed the consequences of density change on the swimming efforts of individuals while diving and investigated the effects on dive duration. Both descent swimming speed and ascent swimming effort were found to be strongly correlated to descent rate during drift dives, enabling the fine-scale monitoring of seal density change over the whole trip. Negatively buoyant seals minimized swimming effort during descents, gliding down at slower speeds, and reduced their ascent swimming effort to maintain a nearly constant swimming speed as their buoyancy increased. One per cent of seal density variation over time was found to induce a 20% variation in swimming effort during dives with direct consequences on dive duration. © 2014. Published by The Company of Biologists Ltd.

Density Driven Removal of Sediment from a Buoyant Muddy Plume

NASA Astrophysics Data System (ADS)

Rouhnia, M.; Strom, K.

2014-12-01

Experiments were conducted to study the effect of settling driven instabilities on sediment removal from hypopycnal plumes. Traditional approaches scale removal rates with particle settling velocity however, it has been suggested that the removal from buoyant suspensions happens at higher rates. The enhancement of removal is likely due to gravitational instabilities, such as fingering, at two-fluid interface. Previous studies have all sought to suppress flocculation, and no simple model exists to predict the removal rates under the effect of such instabilities. This study examines whether or not flocculation hampers instability formation and presents a simple removal rate model accounting for gravitational instabilities. A buoyant suspension of flocculated Kaolinite overlying a base of clear saltwater was investigated in a laboratory tank. Concentration was continuously measured in both layers with a pair of OBS sensors, and interface was monitored with digital cameras. Snapshots from the video were used to measure finger velocity. Samples of flocculated particles at the interface were extracted to retrieve floc size data using a floc camera. Flocculation did not stop creation of settling-driven fingers. A simple cylinder-based force balance model was capable of predicting finger velocity. Analogy of fingering process of fine grained suspensions to thermal plume formation and the concept of Grashof number enabled us to model finger spacing as a function of initial concentration. Finally, from geometry, the effective cross-sectional area was correlated to finger spacing. Reformulating the outward flux expression was done by substitution of finger velocity, rather than particle settling velocity, and finger area instead of total area. A box model along with the proposed outward flux was used to predict the SSC in buoyant layer. The model quantifies removal flux based on the initial SSC and is in good agreement with the experimental data.

Axisymmetric contour dynamics for buoyant vortex rings

NASA Astrophysics Data System (ADS)

Chang, Ching; Llewellyn Smith, Stefan

2017-11-01

Vortex rings are important in many fluid flows in engineering and environmental applications. A family of steady propagating vortex rings including thin-core rings and Hill's spherical vortex was obtained by Norbury (1973). However, the dynamics of vortex rings in the presence of buoyancy has not been investigated yet in detail. When the core of a ring is thin, we may formulate reduced equations using momentum balance for vortex filaments, but that is not the case for ``fat'' rings. In our study, we use contour dynamics to study the time evolution of axisymmetric vortex rings when the density of the fluid inside the ring differs from that of the ambient. Axisymmetry leads to an almost-conserved material variable when the Boussinesq approximation is made. A set of integro-differential equations is solved numerically for these buoyant vortex rings. The same physical settings are also used to run a DNS code and compare to the results from contour dynamics.

Rethinking Recycling in Arcs

NASA Astrophysics Data System (ADS)

Kelemen, P.; Behn, M. D.; Jagoutz, O.

2012-12-01

Hacker et al EPSL 2011 and Behn et al Nature Geosci 2011 investigated pathways for return of buoyant, subducted material to arc crust. These include (1) diapirs rising into the hot mantle wedge, with extensive melts adding a component to arc magmas, (2) flow of material back up a relatively cold "subduction channel", adding solids to the lower crust and small-degree partial melts to the upper crust, (3) flow from the forearc along the base of arc crust, and (4) imbrication of forearc material into arc crust. These processes add felsic, incompatible-element-rich components to arc crust. The flux of incompatible elements such as Th in arc lavas, thought to be mainly recycled from subducted sediments, is > sediment subduction flux. There are large uncertainties: arc crustal growth rates are imprecise; young, primitive arc lavas may not be representative of magmatic flux into arc crust; sediment subduction flux may have varied. Nevertheless, this result is found for all arcs examined, using recently published growth rates. Perhaps arc growth rates that include subduction erosion are systematically overestimated. Instead or in addition, maybe significant Th comes from material other than sediments. Here, we consider the implications of pathways 1-4 for arc growth rates and incompatible element enrichment, in the context of subduction erosion and arc-arc collision. Subducting arc lithologies can become separated, with only felsic components returned to arc crust. Buoyant lithologies are mobile in viscous instabilities at > 700-800°C. Whereas thin layers such as sediments may become mobile all at once, instabilities may periodically strip the hottest parts from the top of thick buoyant layers, replacing them with hot mantle. In arc-arc collision, the top of a subducting plate starts at about 0°C on the seafloor, so heating is slow. In subduction erosion, forearc material in the subducting package can be > 200°C before erosion so buoyant lithologies reach 700-800°C faster, and in larger volumes at a given time. Subduction erosion rarely, if ever, transports significant amounts of buoyant material deep into the convecting mantle. Because buoyant material can remain part of the crust, it may often be a mistake to add all of the eroded material to the observed arc volume to derive crustal growth rates. Buoyancy instabilities during subduction erosion or arc-arc collision will accumulate felsic arc crust. For example, > 50% of Aleutian arc lavas and exposed plutons are more buoyant than mantle peridotite at 700-800°C, 3-4 GPa. The buoyant material has an average of 60-62 wt% SiO2, molar Mg/(Mg+Fe) 0.4-0.5, and trace elements identical to bulk continental crust, though western Aleutian lavas have the most depleted Sr, Nd and Pb isotope ratios of all arc lavas worldwide. In general, density sorting of arc lithologies, and subsequent partial melting as buoyant rocks rise through the mantle wedge or along a subduction channel, could lead to a kind of double and triple distillation. Incompatible elements such as Th would be enriched in arc crust, retaining correlations with isotopic indicators of a recycled sediment component, while Th-poor, dense, mafic lavas and lower crustal cumulates return to the convecting mantle.

A novel technique for real-time estimation of edge pedestal density gradients via reflectometer time delay data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zeng, L., E-mail: zeng@fusion.gat.com; Doyle, E. J.; Rhodes, T. L.

2016-11-15

A new model-based technique for fast estimation of the pedestal electron density gradient has been developed. The technique uses ordinary mode polarization profile reflectometer time delay data and does not require direct profile inversion. Because of its simple data processing, the technique can be readily implemented via a Field-Programmable Gate Array, so as to provide a real-time density gradient estimate, suitable for use in plasma control systems such as envisioned for ITER, and possibly for DIII-D and Experimental Advanced Superconducting Tokamak. The method is based on a simple edge plasma model with a linear pedestal density gradient and low scrape-off-layermore » density. By measuring reflectometer time delays for three adjacent frequencies, the pedestal density gradient can be estimated analytically via the new approach. Using existing DIII-D profile reflectometer data, the estimated density gradients obtained from the new technique are found to be in good agreement with the actual density gradients for a number of dynamic DIII-D plasma conditions.« less

46 CFR 108.697 - Buoyant work vests.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Buoyant work vests. 108.697 Section 108.697 Shipping... EQUIPMENT Miscellaneous Equipment § 108.697 Buoyant work vests. (a) Each buoyant work vest on a unit must be... work vests must be— (1) Used, stowed, and maintained in accordance with the procedures set out in the...

46 CFR 131.870 - Life floats and buoyant apparatus.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Life floats and buoyant apparatus. 131.870 Section 131... OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.870 Life floats and buoyant apparatus. (a) The name of the vessel must be plainly marked or painted on each life float or buoyant apparatus...

46 CFR 131.870 - Life floats and buoyant apparatus.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Life floats and buoyant apparatus. 131.870 Section 131... OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.870 Life floats and buoyant apparatus. (a) The name of the vessel must be plainly marked or painted on each life float or buoyant apparatus...

46 CFR 131.870 - Life floats and buoyant apparatus.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Life floats and buoyant apparatus. 131.870 Section 131... OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.870 Life floats and buoyant apparatus. (a) The name of the vessel must be plainly marked or painted on each life float or buoyant apparatus...

46 CFR 108.697 - Buoyant work vests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Buoyant work vests. 108.697 Section 108.697 Shipping... EQUIPMENT Miscellaneous Equipment § 108.697 Buoyant work vests. (a) Each buoyant work vest on a unit must be... work vests must be— (1) Used, stowed, and maintained in accordance with the procedures set out in the...

46 CFR 108.697 - Buoyant work vests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Buoyant work vests. 108.697 Section 108.697 Shipping... EQUIPMENT Miscellaneous Equipment § 108.697 Buoyant work vests. (a) Each buoyant work vest on a unit must be... work vests must be— (1) Used, stowed, and maintained in accordance with the procedures set out in the...

46 CFR 108.697 - Buoyant work vests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Buoyant work vests. 108.697 Section 108.697 Shipping... EQUIPMENT Miscellaneous Equipment § 108.697 Buoyant work vests. (a) Each buoyant work vest on a unit must be... work vests must be— (1) Used, stowed, and maintained in accordance with the procedures set out in the...

46 CFR 108.697 - Buoyant work vests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Buoyant work vests. 108.697 Section 108.697 Shipping... EQUIPMENT Miscellaneous Equipment § 108.697 Buoyant work vests. (a) Each buoyant work vest on a unit must be... work vests must be— (1) Used, stowed, and maintained in accordance with the procedures set out in the...

46 CFR 131.870 - Life floats and buoyant apparatus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.870 Life floats and buoyant apparatus. (a) The name of the vessel must be plainly marked or painted on each life float or buoyant apparatus... 46 Shipping 4 2011-10-01 2011-10-01 false Life floats and buoyant apparatus. 131.870 Section 131...

46 CFR 131.870 - Life floats and buoyant apparatus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.870 Life floats and buoyant apparatus. (a) The name of the vessel must be plainly marked or painted on each life float or buoyant apparatus... 46 Shipping 4 2010-10-01 2010-10-01 false Life floats and buoyant apparatus. 131.870 Section 131...

Baculite 3D Modeling: a New Method for Computing Buoyancy, Stability, and Orientation with Implications for Ectocochleate Cephalopod Hydrostatics

NASA Astrophysics Data System (ADS)

Peterman, D. J.; Barton, C. C.

2017-12-01

Ectocochleate (external) cephalopod shells are comprised of a body chamber which houses the organism's soft parts and the phragmocone which consists of a series of progressively larger chambers (camerae) divided by septa. The phragmocone is used as a passive gas float for buoyancy regulation. The soft body and the mineralized shell are denser than water and are negatively buoyant while the phragmocone is positively buoyant due to some fraction of gas in its chambers. This provides a neutrally buoyant condition when the total mass of the organism is equal to the mass of the displaced water. The static orientation of the organism occurs when the centers of buoyancy and mass are vertically aligned and stability is determined by their degree of separation. Three-dimensional modeling of a specimen of Baculites compressus (which has a straight conical shell) was performed using Autodesk Meshmixer, Netfabb ®, Blender 2.78, and MeshLab. The initial 3D mesh shapefile was created by Autodesk ReCap 360™ photogrammetry software. The specimen requirements for the models include: an external shell (ideally complete, otherwise approximated), a septum showing lower order frilling, and a suture pattern to reconstruct the higher order septal frilling (for complex septa). Volumes and centers of mass/buoyancy were calculated with MeshLab in order to determine neutrality, stability, and orientation. Our method can be used to investigate the influence of morphological features on these hydrostatic properties of ectocochleate cephalopods and also the paleoecological implications of different morphotypes. Baculites compressus, is found to assume relatively stable vertical orientations when the shell is positively or neutrally buoyant. By arbitrarily flooding all chambers, the ammonite becomes negatively buoyant, and the centers of buoyancy and mass virtually coincide. This reduces stability but allows the living ammonite to assume a larger range of orientations, including horizontal, possibly by the active use of its hyponome (funnel used for jet propulsion). The geometry of the shell and amount of cameral gas, relative to the soft body and cameral liquid, have the greatest influence on the hydrostatic properties of ectocochleate cephalopods due to their large differences in density relative to that of seawater.

46 CFR 117.137 - Stowage of life floats and buoyant apparatus.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Stowage of life floats and buoyant apparatus. 117.137... EQUIPMENT AND ARRANGEMENTS Survival Craft Arrangements and Equipment § 117.137 Stowage of life floats and buoyant apparatus. (a) In addition to meeting § 117.130, each life float and buoyant apparatus must be...

46 CFR 117.137 - Stowage of life floats and buoyant apparatus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Stowage of life floats and buoyant apparatus. 117.137... EQUIPMENT AND ARRANGEMENTS Survival Craft Arrangements and Equipment § 117.137 Stowage of life floats and buoyant apparatus. (a) In addition to meeting § 117.130, each life float and buoyant apparatus must be...

46 CFR 117.137 - Stowage of life floats and buoyant apparatus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Stowage of life floats and buoyant apparatus. 117.137... EQUIPMENT AND ARRANGEMENTS Survival Craft Arrangements and Equipment § 117.137 Stowage of life floats and buoyant apparatus. (a) In addition to meeting § 117.130, each life float and buoyant apparatus must be...

46 CFR 160.010-5 - Buoyant apparatus with plastic foam buoyancy.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 6 2013-10-01 2013-10-01 false Buoyant apparatus with plastic foam buoyancy. 160.010-5... Vessels § 160.010-5 Buoyant apparatus with plastic foam buoyancy. (a) Buoyant apparatus with plastic foam buoyancy must have a plastic foam body with an external protective covering. The body may be reinforced as...

46 CFR 160.010-5 - Buoyant apparatus with plastic foam buoyancy.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Buoyant apparatus with plastic foam buoyancy. 160.010-5... Vessels § 160.010-5 Buoyant apparatus with plastic foam buoyancy. (a) Buoyant apparatus with plastic foam buoyancy must have a plastic foam body with an external protective covering. The body may be reinforced as...

46 CFR 160.010-5 - Buoyant apparatus with plastic foam buoyancy.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Buoyant apparatus with plastic foam buoyancy. 160.010-5... Vessels § 160.010-5 Buoyant apparatus with plastic foam buoyancy. (a) Buoyant apparatus with plastic foam buoyancy must have a plastic foam body with an external protective covering. The body may be reinforced as...

Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles

PubMed Central

Minciacchi, Valentina R.; You, Sungyong; Spinelli, Cristiana; Morley, Samantha; Zandian, Mandana; Aspuria, Paul-Joseph; Cavallini, Lorenzo; Ciardiello, Chiara; Sobreiro, Mariana Reis; Morello, Matteo; Kharmate, Geetanjali; Jang, Su Chul; Kim, Dae-Kyum; Hosseini-Beheshti, Elham; Guns, Emma Tomlinson; Gleave, Martin; Gho, Yong Song; Mathivanan, Suresh; Yang, Wei; Freeman, Michael R.; Di Vizio, Dolores

2015-01-01

Large oncosomes (LO) are atypically large (1-10μm diameter) cancer-derived extracellular vesicles (EVs), originating from the shedding of membrane blebs and associated with advanced disease. We report that 25% of the proteins, identified by a quantitative proteomics analysis, are differentially represented in large and nano-sized EVs from prostate cancer cells. Proteins enriched in large EVs included enzymes involved in glucose, glutamine and amino acid metabolism, all metabolic processes relevant to cancer. Glutamine metabolism was altered in cancer cells exposed to large EVs, an effect that was not observed upon treatment with exosomes. Large EVs exhibited discrete buoyant densities in iodixanol (OptiPrepTM) gradients. Fluorescent microscopy of large EVs revealed an appearance consistent with LO morphology, indicating that these structures can be categorized as LO. Among the proteins enriched in LO, cytokeratin 18 (CK18) was one of the most abundant (within the top 5th percentile) and was used to develop an assay to detect LO in the circulation and tissues of mice and patients with prostate cancer. These observations indicate that LO represent a discrete EV type that may play a distinct role in tumor progression and that may be a source of cancer-specific markers. PMID:25857301

Numerical modeling of physical vapor transport under microgravity conditions: Effect of thermal creep and stress

NASA Technical Reports Server (NTRS)

Mackowski, Daniel W.; Knight, Roy W.

1993-01-01

One of the most promising applications of microgravity (micro-g) environments is the manufacture of exotic and high-quality crystals in closed cylindrical ampoules using physical vapor transport (PVT) processes. The quality enhancements are believed to be due to the absence of buoyant convection in the weightless environment - resulting in diffusion-limited transport of the vapor. In a typical experiment, solid-phase sample material is initially contained at one end of the ampoule. The sample is made to sublime into the vapor phase and deposit onto the opposite end by maintaining the source at an elevated temperature with respect to the deposit. Identification of the physical factors governing both the rates and uniformity of crystal growth, and the optimization of the micro-g technology, will require an accurate modeling of the vapor transport within the ampoule. Previous micro-g modeling efforts have approached the problem from a 'classical' convective/diffusion formulation, in which convection is driven by the action of buoyancy on thermal and solutal density differences. The general conclusion of these works have been that in low gravity environments the effect of buoyancy on vapor transport is negligible, and vapor transport occurs in a diffusion-limited mode. However, it has been recently recognized than in the non-isothermal (and often low total pressure) conditions encountered in ampoules, the commonly-assumed no-slip boundary condition to the differential equations governing fluid motion can be grossly unrepresentative of the actual situation. Specifically, the temperature gradients can give rise to thermal creep flows at the ampoule side walls. In addition, temperature gradients in the vapor itself can, through the action of thermal stress, lead to bulk fluid convection.

Connections between density, wall-normal velocity, and coherent structure in a heated turbulent boundary layer

NASA Astrophysics Data System (ADS)

Saxton-Fox, Theresa; Gordeyev, Stanislav; Smith, Adam; McKeon, Beverley

2015-11-01

Strong density gradients associated with turbulent structure were measured in a mildly heated turbulent boundary layer using an optical sensor (Malley probe). The Malley probe measured index of refraction gradients integrated along the wall-normal direction, which, due to the proportionality of index of refraction and density in air, was equivalently an integral measure of density gradients. The integral output was observed to be dominated by strong, localized density gradients. Conditional averaging and Pearson correlations identified connections between the streamwise gradient of density and the streamwise gradient of wall-normal velocity. The trends were suggestive of a process of pick-up and transport of heat away from the wall. Additionally, by considering the density field as a passive marker of structure, the role of the wall-normal velocity in shaping turbulent structure in a sheared flow was examined. Connections were developed between sharp gradients in the density and flow fields and strong vertical velocity fluctuations. This research is made possible by the Department of Defense through the National Defense & Engineering Graduate Fellowship (NDSEG) Program and by the Air Force Office of Scientific Research Grant # FA9550-12-1-0060.

Cluster kinetics model of particle separation in vibrated granular media.

PubMed

McCoy, Benjamin J; Madras, Giridhar

2006-01-01

We model the Brazil-nut effect (BNE) by hypothesizing that granules form clusters that fragment and aggregate. This provides a heterogeneous medium in which the immersed intruder particle rises (BNE) or sinks (reverse BNE) according to relative convection currents and buoyant and drag forces. A simple relationship proposed for viscous drag in terms of the vibrational intensity and the particle to grain density ratio allows simulation of published experimental data for rise and sink times as functions of particle radius, initial depth of the particle, and particle-grain density ratio. The proposed model correctly describes the experimentally observed maximum in risetime.

Existence, uniqueness and regularity of a time-periodic probability density distribution arising in a sedimentation-diffusion problem

NASA Technical Reports Server (NTRS)

Nitsche, Ludwig C.; Nitsche, Johannes M.; Brenner, Howard

1988-01-01

The sedimentation and diffusion of a nonneutrally buoyant Brownian particle in vertical fluid-filled cylinder of finite length which is instantaneously inverted at regular intervals are investigated analytically. A one-dimensional convective-diffusive equation is derived to describe the temporal and spatial evolution of the probability density; a periodicity condition is formulated; the applicability of Fredholm theory is established; and the parameter-space regions are determined within which the existence and uniqueness of solutions are guaranteed. Numerical results for sample problems are presented graphically and briefly characterized.

The serine proteinase inhibitory proteins of the chondrodystrophoid (beagle) and non-chondrodystrophoid (greyhound) canine intervertebral disc.

PubMed

Melrose, J; Taylor, T K; Ghosh, P

1997-06-01

Trypsin inhibitory proteins of low buoyant density (p < or = 1.35 g/mL) fractions were prepared by CsCl density gradient ultracentrifugation of 4 M guanidinium hydrochloride extracts of lumbar beagle and greyhound annulus fibrosus and nucleus pulposus from animals aged 1 to 6 years. Affinity blotting with biotinylated trypsin was used to identify active trypsin inhibitory protein species; these species were also identified immunologically by Western blotting using antibodies against bovine pancreatic trypsin inhibitor (BPTI), and human inter-alpha-trypsin inhibitor (ITI). None of the trypsin inhibitory species evident in Western blots were reactive with anti-human alpha1-proteinase inhibitor (alpha-1-PI), alpha2-macroglobulin or secretory leucocyte proteinase inhibitor. The greyhound intervertebral disc samples generally had higher levels of active trypsin inhibitor species per unit weight of tissue extracted, and a more extensive range of inhibitor species. Inhibitor species of 30, 32, 34 kDa were identified in both beagle and greyhound intervertebral disc samples; these species were generally most prominent in the annulus fibrosus samples. In contrast, the nucleus pulposus samples contained relatively large trypsin inhibitor species; the anti-BPTI detected an inhibitor species of approximately 85-90 kDa; anti-ITI detected species of 120-250 kDa; biotinylated trypsin detected species of 60-110 kDa. A small molecular mass trypsin inhibitor species of 6 kDa, which was of similar mobility to BPTI, was also detected in annulus fibrosus samples; however, this species did not react with anti-BPTI.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arévalo, P.; Churazov, E.; Zhuravleva, I.

X-ray images of galaxy clusters and gas-rich elliptical galaxies show a wealth of small-scale features that reflect fluctuations in density and/or temperature of the intracluster medium. In this paper we study these fluctuations in M87/Virgo to establish whether sound waves/shocks, bubbles, or uplifted cold gas dominate the structure. We exploit the strong dependence of the emissivity on density and temperature in different energy bands to distinguish between these processes. Using simulations we demonstrate that our analysis recovers the leading type of fluctuation even in the presence of projection effects and temperature gradients. We confirm the isobaric nature of cool filamentsmore » of gas entrained by buoyantly rising bubbles, extending to 7′ to the east and southwest, and the adiabatic nature of the weak shocks at 40″ and 3′ from the center. For features of ∼5–10 kpc, we show that the central 4′ × 4′ region is dominated by cool structures in pressure equilibrium with the ambient hotter gas while up to 30% of the variance in this region can be ascribed to adiabatic fluctuations. The remaining part of the central 14′ × 14′ region, excluding the arms and shocks described above, is dominated by apparently isothermal fluctuations (bubbles) with a possible admixture (at the level of ∼30%) of adiabatic (sound waves) and by isobaric structures. Larger features, of about 30 kpc, show a stronger contribution from isobaric fluctuations. The results broadly agree with a model based on feedback from an active galactic nucleus mediated by bubbles of relativistic plasma.« less

Simulating the interaction of jets with the intracluster medium

NASA Astrophysics Data System (ADS)

Weinberger, Rainer; Ehlert, Kristian; Pfrommer, Christoph; Pakmor, Rüdiger; Springel, Volker

2017-10-01

Jets from supermassive black holes in the centres of galaxy clusters are a potential candidate for moderating gas cooling and subsequent star formation through depositing energy in the intracluster gas. In this work, we simulate the jet-intracluster medium interaction using the moving-mesh magnetohydrodynamics code arepo. Our model injects supersonic, low-density, collimated and magnetized outflows in cluster centres, which are then stopped by the surrounding gas, thermalize and inflate low-density cavities filled with cosmic rays. We perform high-resolution, non-radiative simulations of the lobe creation, expansion and disruption, and find that its dynamical evolution is in qualitative agreement with simulations of idealized low-density cavities that are dominated by a large-scale Rayleigh-Taylor instability. The buoyant rising of the lobe does not create energetically significant small-scale chaotic motion in a volume-filling fashion, but rather a systematic upward motion in the wake of the lobe and a corresponding back-flow antiparallel to it. We find that, overall, 50 per cent of the injected energy ends up in material that is not part of the lobe, and about 25 per cent remains in the inner 100 kpc. We conclude that jet-inflated, buoyantly rising cavities drive systematic gas motions that play an important role in heating the central regions, while mixing of lobe material is subdominant. Encouragingly, the main mechanisms responsible for this energy deposition can be modelled already at resolutions within reach in future, high-resolution cosmological simulations of galaxy clusters.

Single fiber lignin distributions based on the density gradient column method

Treesearch

Brian Boyer; Alan W. Rudie

2007-01-01

The density gradient column method was used to determine the effects of uniform and non-uniform pulping processes on variation in individual fiber lignin concentrations of the resulting pulps. A density gradient column uses solvents of different densities and a mixing process to produce a column of liquid with a smooth transition from higher density at the bottom to...

46 CFR 160.052-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... instruments of closure for buoyant vests. 160.052-3a Section 160.052-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests. (a...

46 CFR 160.060-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... instruments of closure for buoyant vests. 160.060-3a Section 160.060-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests...

46 CFR 160.060-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... instruments of closure for buoyant vests. 160.060-3a Section 160.060-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests...

46 CFR 160.052-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... instruments of closure for buoyant vests. 160.052-3a Section 160.052-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests. (a...

46 CFR 160.060-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... instruments of closure for buoyant vests. 160.060-3a Section 160.060-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests...

46 CFR 160.060-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... instruments of closure for buoyant vests. 160.060-3a Section 160.060-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests...

46 CFR 160.052-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... instruments of closure for buoyant vests. 160.052-3a Section 160.052-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests. (a...

46 CFR 160.052-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... instruments of closure for buoyant vests. 160.052-3a Section 160.052-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests. (a...

SUPERGRANULATION AS THE LARGEST BUOYANTLY DRIVEN CONVECTIVE SCALE OF THE SUN

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cossette, Jean-Francois; Rast, Mark P.

The origin of solar supergranulation remains a mystery. Unlike granulation, the size of which is comparable to both the thickness of the radiative boundary layer and local scale-height in the photosphere, supergranulation does not reflect any obvious length scale of the solar convection zone. Moreover, recent observations of flows in the photosphere using Doppler imaging or correlation or feature tracking show a monotonic decrease in horizontal flow power at scales larger than supergranulation. Both local area and global spherical shell simulations of solar convection by contrast show the opposite, an increase in horizontal flow amplitudes to a low wavenumber. Wemore » examine these disparities and investigate how the solar supergranulation may arise as a consequence of nonlocal heat transport by cool diving plumes. Using three-dimensional anelastic simulations with surface driving, we show that the kinetic energy of the largest convective scales in the upper layers of a stratified domain reflects the depth of transition from strong buoyant driving to adiabatic stratification below caused by the dilution of the granular downflows. This depth is quite shallow because of the rapid increase of the mean density below the photosphere. We interpret the observed monotonic decrease in solar convective power at scales larger than supergranulation to be a consequence of this rapid transition, with the supergranular scale the largest buoyantly driven mode of convection in the Sun.« less

Suppression of Buoyancy in Gaseous Media at High Temperatures

NASA Technical Reports Server (NTRS)

Gokoglu, Suleyman A.; Kuczmarski, Maria A.

2003-01-01

Consider a rectangular box filled with a fluid having a heated bottom and a cold top surface, and insulated side-walls (Benard problem). As the temperature difference between the horizontal top and bottom surfaces increases, a critical condition, defined quantitatively by the Rayleigh number, is reached beyond which density stratification can no longer be sustained by conduction and the fluid disrupts from its stable, quiescent state into an unstable, convective mode in which lighter and heavier gas mix. This paper suggests that such a statement is not necessarily true for gaseous media under normalized temperature differences that are much larger than justifiable for the Boussinesq approximation! In fact, there may be situations where a system cannot ever be made unstable with respect to the onset on buoyant convection no matter how large the temperature (density) difference becomes at a given pressure even under normal gravity! This unexpected behavior is primarily attributed to highly temperature-sensitive kinematic viscosity which counteracts the tendency toward instability and dampens convection by making the gas more viscous at higher temperatures. This compensation of the buoyant force by the viscous force exhibits itself by the formation of a peak hot-surface temperature beyond which a system will tend to be more stable as the hot-surface temperature increases.

Particle dynamics and pattern formation in a rotating suspension of positively buoyant particles

NASA Astrophysics Data System (ADS)

Konidena, Sudarshan; Lee, Jonghoon; Reddy, K. Anki; Singh, Anugrah

2018-04-01

Numerical simulations of positively buoyant suspension in a horizontally rotating cylinder were performed to study the formation of radial and axial patterns. The order parameter for the low-frequency segregated phase and dispersed phase is similar to that predicted for the settling suspension by Lee and Ladd [J. Fluid Mech. 577, 183 (2007), 10.1017/S002211200700465X], which is the average angular velocity of the particles. The particle density profiles for axial bands in the buoyancy-dominated phase shows an amplitude equivalent to the diameter of the cylinder. Axial density profiles show sinusoidal behavior for the drag-dominant phase and oscillating sinusoidal behavior for the centrifugal-force-dominant phase. Results also indicate that the traveling bands are formed as a consequence of the inhomogeneous distribution of particles arising from a certain imbalance of drag, buoyancy, and centrifugal forces. In the centrifugal limit, particles move towards the center of the cylinder, aggregating to form a dense core of particles with its axis coinciding with that of the rotating cylinder, a behavior which is in contrast to the sedimenting particles. The particle distribution patterns obtained from the simulations are found to be in good agreement with the experiments of Kalyankar et al. [Phys. Fluids 20, 083301 (2008), 10.1063/1.2970156].

Effects of Planetary Thermal Structure on the Ascent and Cooling of Magma on Venus

NASA Technical Reports Server (NTRS)

Sakimoto, Susan E. H.; Zuber, Maria T.

1995-01-01

Magellan radar images of the surface of Venus show a spatially broad distribution of volcanic features. Models of magmatic ascent processes to planetary surfaces indicate that the thermal structure of the interior significantly influences the rate of magmatic cooling and thus the amount of magma that can be transported to the surface before solidification. In order to understand which aspects of planetary thermal structure have the greatest influence on the cooling of buoyantly ascending magma, we have constructed magma cooling profiles for a plutonic ascent mechanism, and evaluated the profiles for variations in the surface and mantle temperature, surface temperature gradient, and thermal gradient curvature. Results show that, for a wide variety of thermal conditions, smaller and slower magma bodies are capable of reaching the surface on Venus compared to Earth, primarily due to the higher surface temperature of Venus. Little to no effect on the cooling and transport of magma are found to result from elevated mantle temperatures, elevation-dependent surface temperature variations, or details of the thermal gradient curvature. The enhanced tendency of magma to reach the surface on Venus may provide at least a partial explanation for the extensive spatial distribution of observed volcanism on the surface.

Heterotrophic Proteobacteria in the vicinity of diffuse hydrothermal venting.

PubMed

Meier, Dimitri V; Bach, Wolfgang; Girguis, Peter R; Gruber-Vodicka, Harald R; Reeves, Eoghan P; Richter, Michael; Vidoudez, Charles; Amann, Rudolf; Meyerdierks, Anke

2016-12-01

Deep-sea hydrothermal vents are highly dynamic habitats characterized by steep temperature and chemical gradients. The oxidation of reduced compounds dissolved in the venting fluids fuels primary production providing the basis for extensive life. Until recently studies of microbial vent communities have focused primarily on chemolithoautotrophic organisms. In our study, we targeted the change of microbial community compositions along mixing gradients, focusing on distribution and capabilities of heterotrophic microorganisms. Samples were retrieved from different venting areas within the Menez Gwen hydrothermal field, taken along mixing gradients, including diffuse fluid discharge points, their immediate surroundings and the buoyant parts of hydrothermal plumes. High throughput 16S rRNA gene amplicon sequencing, fluorescence in situ hybridization, and targeted metagenome analysis were combined with geochemical analyses. Close to diffuse venting orifices dominated by chemolithoautotrophic Epsilonproteobacteria, in areas where environmental conditions still supported chemolithoautotrophic processes, we detected microbial communities enriched for versatile heterotrophic Alpha- and Gammaproteobacteria. The potential for alkane degradation could be shown for several genera and yet uncultured clades. We propose that hotspots of chemolithoautotrophic life support a 'belt' of heterotrophic bacteria significantly different from the dominating oligotrophic microbiota of the deep sea. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

46 CFR 160.047-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... instruments of closure for buoyant vests. 160.047-3a Section 160.047-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests. (a...

46 CFR 160.047-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... instruments of closure for buoyant vests. 160.047-3a Section 160.047-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests. (a...

46 CFR 160.047-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... instruments of closure for buoyant vests. 160.047-3a Section 160.047-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests. (a...

46 CFR 160.047-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... instruments of closure for buoyant vests. 160.047-3a Section 160.047-3a Shipping COAST GUARD, DEPARTMENT OF... LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-3a Materials—Dee ring and snap hook assemblies and other instruments of closure for buoyant vests. (a...

Effects of Submarine Groundwater Discharge (SGD) on the Growth of the Lobe Coral Porites lobata in Maunalua Bay, Hawaii.

NASA Astrophysics Data System (ADS)

Lubarsky, K.

2016-02-01

Submarine groundwater discharge (SGD) constitutes a large percentage of the freshwater inputs onto coastal coral reefs on high islands such as the Hawaiian Islands, although the impact of SGD on coral reef health is currently understudied. In Maunalua Bay, on Oahu, Hawaii, SGD is discharged onto shallow reef flats from discrete seeps, creating natural gradients of water chemistry across the reef flat. We used this system to investigate rates of growth of the lobe coral Porites lobata across a gradient of SGD influence at two study sites within the bay, and to characterize the variation in water chemistry gradient over space and time due to SGD. SGD input at these sites is tidally modulated, and the groundwater itself is brackish and extremely nutrient-rich (mean=190 μM NO3- at the Black Point study site, mean=40 μM NO3- at Wailupe Beach Park), with distinct carbonate signatures at both study sites. Coral nubbins were placed across the gradient for 6 months, and growth was measured using three metrics: surface area (photo analysis), buoyant weight, and linear extension. Various chemical parameters, including pH, salinity, total alkalinity, nutrients, and chlorphyll were sampled at the same locations across the gradient over 24 hour periods in the spring and fall in order to capture spatial and temporal variation in water chemistry due to the SGD plume. Spatial patterns and temporal variation in water chemistry were correlated with the observed spatial patterns in coral growth across the SGD gradient.

A straightforward method for measuring the range of apparent density of microplastics.

PubMed

Li, Lingyun; Li, Mengmeng; Deng, Hua; Cai, Li; Cai, Huiwen; Yan, Beizhan; Hu, Jun; Shi, Huahong

2018-10-15

Density of microplastics has been regarded as the primary property that affect the distribution and bioavailability of microplastics in the water column. For measuring the density of microplastis, we developed a simple and rapid method based on density gradient solutions. In this study, we tested four solvents to make the density gradient solutions, i.e., ethanol (0.8 g/cm 3 ), ultrapure water (1.0 g/cm 3 ), saturated NaI (1.8 g/cm 3 ) and ZnCl 2 (1.8 g/cm 3 ). Density of microplastics was measured via observing the float or sink status in the density gradient solutions. We found that density gradient solutions made from ZnCl 2 had a larger uncertainty in measuring density than that from NaI, most likely due to a higher surface tension of ZnCl 2 solution. Solutions made from ethanol, ultrapure water, and NaI showed consistent density results with listed densities of commercial products, indicating that these density gradient solutions were suitable for measuring microplastics with a density range of 0.8-1.8 g/cm 3 . Copyright © 2018 Elsevier B.V. All rights reserved.

A two-layer model for buoyant inertial displacement flows in inclined pipes

NASA Astrophysics Data System (ADS)

Etrati, Ali; Frigaard, Ian A.

2018-02-01

We investigate the inertial flows found in buoyant miscible displacements using a two-layer model. From displacement flow experiments in inclined pipes, it has been observed that for significant ranges of Fr and Re cos β/Fr, a two-layer, stratified flow develops with the heavier fluid moving at the bottom of the pipe. Due to significant inertial effects, thin-film/lubrication models developed for laminar, viscous flows are not effective for predicting these flows. Here we develop a displacement model that addresses this shortcoming. The complete model for the displacement flow consists of mass and momentum equations for each fluid, resulting in a set of four non-linear equations. By integrating over each layer and eliminating the pressure gradient, we reduce the system to two equations for the area and mean velocity of the heavy fluid layer. The wall and interfacial stresses appear as source terms in the reduced system. The final system of equations is solved numerically using a robust, shock-capturing scheme. The equations are stabilized to remove non-physical instabilities. A linear stability analysis is able to predict the onset of instabilities at the interface and together with numerical solution, is used to study displacement effectiveness over different parametric regimes. Backflow and instability onset predictions are made for different viscosity ratios.

46 CFR 160.010-2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant Vessels § 160.010-2 Definitions. Buoyant apparatus. Buoyant apparatus is flotation equipment (other than lifeboats, liferafts, and personal...

46 CFR 160.048-7 - Procedure for approval.

Code of Federal Regulations, 2010 CFR

2010-10-01

... subpart, which are 2 inches thick, and which are filled with kapok or fibrous glass in accordance with... buoyant cushion more than 2 inches thick; or any buoyant cushion having a different shape. (c) A buoyant...

Dynamical consequences of compositional and thermal density stratification beneath spreading centers

NASA Technical Reports Server (NTRS)

Sotin, C.; Parmentier, E. M.

1989-01-01

Dynamical consequences of compositional buoyancy and the combined effects of compositional and thermal buoyancy on mantle flow and crustal production are explored. The results show that for a low enough mantle viscosity, buoyant upwelling can significantly enhance the crustal thickness relative to that which would be produced by plate spreading alone, while for a mantle viscosity of 10 to the 22nd Pa s, upwelling due to plate spreading is dominant and crustal thickness is predicted to be a function of spreading rate. The results indicate that thermal and compositional density variations result in opposing buoyancy forces that can cause time-dependent upwelling.

Investigation of Vibrational Control of the Bridgman Crystal Growth Technique

NASA Technical Reports Server (NTRS)

Fedoseyev, Alexandre I.; Alexander, J. I. D.; Feigelson, R. S.; Zharikov, E. V.; Ostrogorsky, A. G.; Marin, C.; Volz, M. P.; Kansa, E. J.; Friedman, M. J.

2001-01-01

The character of natural buoyant convection in rigidly contained inhomogeneous fluids can be drastically altered by vibrating the container. Vibrations are expected to play a crucial influence on heat and mass transfer onboard the International Space Station (ISS). It is becoming evident that substantial vibrations will exist on the ISS in the wide frequency spectrum. In general, vibrational flows are very complex and governed by many parameters. In many terrestrial crystal growth situations, convective transport of heat and constituent components is dominated by buoyancy driven convection arising from compositional and thermal gradients. Thus, it may be concluded that vibro-convective flow can potentially be used to influence and even control transport in some crystal growth situations.

A modified gradient approach for the growth of low-density InAs quantum dot molecules by molecular beam epitaxy

NASA Astrophysics Data System (ADS)

Sharma, Nandlal; Reuter, Dirk

2017-11-01

Two vertically stacked quantum dots that are electronically coupled, so called quantum dot molecules, are of great interest for the realization of solid state building blocks for quantum communication networks. We present a modified gradient approach to realize InAs quantum dot molecules with a low areal density so that single quantum dot molecules can be optically addressed. The individual quantum dot layers were prepared by solid source molecular beam epitaxy depositing InAs on GaAs(100). The bottom quantum dot layer has been grown without substrate rotation resulting in an In-gradient across the surface, which translated into a density gradient with low quantum dot density in a certain region of the wafer. For the top quantum dot layer, separated from the bottom quantum dot layer by a 6 nm thick GaAs barrier, various InAs amounts were deposited without an In-gradient. In spite of the absence of an In-gradient, a pronounced density gradient is observed for the top quantum dots. Even for an In-amount slightly below the critical thickness for a single dot layer, a density gradient in the top quantum dot layer, which seems to reproduce the density gradient in the bottom layer, is observed. For more or less In, respectively, deviations from this behavior occur. We suggest that the obvious influence of the bottom quantum dot layer on the growth of the top quantum dots is due to the strain field induced by the buried dots.

Effects of particle-fluid density ratio on the interactions between the turbulent channel flow and finite-size particles

NASA Astrophysics Data System (ADS)

Yu, Zhaosheng; Lin, Zhaowu; Shao, Xueming; Wang, Lian-Ping

2017-09-01

A parallel direct-forcing fictitious domain method is employed to perform fully resolved numerical simulations of turbulent channel flow laden with finite-size particles. The effects of the particle-fluid density ratio on the turbulence modulation in the channel flow are investigated at the friction Reynolds number of 180, the particle volume fraction of 0.84 % , and the particle-fluid density ratio ranging from 1 to 104.2. The results show that the variation of the flow drag with the particle-fluid density ratio is not monotonic, with a larger flow drag for the density ratio of 10.42, compared to those of unity and 104.2. A significant drag reduction by the particles is observed for large particle-fluid density ratios during the transient stage, but not at the statistically stationary stage. The intensity of particle velocity fluctuations generally decreases with increasing particle inertia, except that the particle streamwise root-mean-square velocity and streamwise-transverse velocity correlation in the near-wall region are largest at the density ratio of the order of 10. The averaged momentum equations are derived with the spatial averaging theorem and are used to analyze the mechanisms for the effects of the particles on the flow drag. The results indicate that the drag-reduction effect due to the decrease in the fluid Reynolds shear stress is counteracted by the drag-enhancement effect due to the increase in the total particle stress or the interphase drag force for the large particle-inertia case. The sum of the total Reynolds stress and particle inner stress contributions to the flow drag is largest at the density ratio of the order of 10, which is the reason for the largest flow drag at this density ratio. The interphase drag force obtained from the averaged momentum equation (the balance theory) is significantly smaller than (but agrees qualitatively with) that from the empirical drag formula based on the phase-averaged slip velocity for large density ratios. For the neutrally buoyant case, the balance theory predicts a positive interphase force on the particles arising from the negative gradient of the particle inner stress, which cannot be predicted by the drag formula based on the phase-averaged slip velocity. In addition, our results show that both particle collision and particle-turbulence interaction play roles in the formation of the inhomogeneous distribution of the particles at the density ratio of the order of 10.

Effects of particle-fluid density ratio on the interactions between the turbulent channel flow and finite-size particles.

PubMed

Yu, Zhaosheng; Lin, Zhaowu; Shao, Xueming; Wang, Lian-Ping

2017-09-01

A parallel direct-forcing fictitious domain method is employed to perform fully resolved numerical simulations of turbulent channel flow laden with finite-size particles. The effects of the particle-fluid density ratio on the turbulence modulation in the channel flow are investigated at the friction Reynolds number of 180, the particle volume fraction of 0.84%, and the particle-fluid density ratio ranging from 1 to 104.2. The results show that the variation of the flow drag with the particle-fluid density ratio is not monotonic, with a larger flow drag for the density ratio of 10.42, compared to those of unity and 104.2. A significant drag reduction by the particles is observed for large particle-fluid density ratios during the transient stage, but not at the statistically stationary stage. The intensity of particle velocity fluctuations generally decreases with increasing particle inertia, except that the particle streamwise root-mean-square velocity and streamwise-transverse velocity correlation in the near-wall region are largest at the density ratio of the order of 10. The averaged momentum equations are derived with the spatial averaging theorem and are used to analyze the mechanisms for the effects of the particles on the flow drag. The results indicate that the drag-reduction effect due to the decrease in the fluid Reynolds shear stress is counteracted by the drag-enhancement effect due to the increase in the total particle stress or the interphase drag force for the large particle-inertia case. The sum of the total Reynolds stress and particle inner stress contributions to the flow drag is largest at the density ratio of the order of 10, which is the reason for the largest flow drag at this density ratio. The interphase drag force obtained from the averaged momentum equation (the balance theory) is significantly smaller than (but agrees qualitatively with) that from the empirical drag formula based on the phase-averaged slip velocity for large density ratios. For the neutrally buoyant case, the balance theory predicts a positive interphase force on the particles arising from the negative gradient of the particle inner stress, which cannot be predicted by the drag formula based on the phase-averaged slip velocity. In addition, our results show that both particle collision and particle-turbulence interaction play roles in the formation of the inhomogeneous distribution of the particles at the density ratio of the order of 10.

Density Sorting During the Evolution of Continental Crust

NASA Astrophysics Data System (ADS)

Kelemen, P. B.; Behn, M. D.; Hacker, B. R.

2015-12-01

We consider two settings - in addition to "delamination" of arc lower crust - in which dense, mafic eclogites founder into the convecting mantle while buoyant, felsic lithologies accumulate at the base of evolving continental crust. Arc processes play a central role in generating continental crust, but it remains uncertain how basaltic arc crust is transformed to andesitic continental crust. Dense, SiO2-poor products of fractionation may founder from the base of arc crust by "delamination", but lower arc crust after delamination has significantly different trace elements compared to lower continental crust (LCC). In an alternative model, buoyant magmatic rocks generated at arcs are first subducted, mainly via subduction erosion. Upon heating, these buoyant lithologies ascend through the mantle wedge or along a subduction channel, and are "relaminated" at
the base of overlying crust (e.g., Hacker et al EPSL 11, AREPS 15). Average buoyant lavas and plutons
for the Aleutians, Izu-Bonin-Marianas, Kohistan and Talkeetna arcs fall within the range of estimated LCC major and trace elements. Relamination is more efficient in generating continental crust than delamination. Himalayan cross-sections show Indian crust thrust beneath Tibetan crust, with no intervening mantle. There is a horizontal Moho at ca 80 km depth, extending from thickened Indian crust, across the region where Tibetan crust overlies Indian crust, into thickened Tibetan crust. About half the subducted Indian crust is present, whereas the other half is missing. Data (Vp/Vs; Miocene lavas formed by interaction of continental crust with mantle; xenolith thermometry) indicate 1000°C or more from ca 50 km depth to the Moho since the Miocene. We build on earlier studies (LePichon et al Tectonics 92, T'phys 97; Schulte-Pelkum et al Nature 05; Monsalve et al JGR 08) to advance the hypothesis that rapid growth of garnet occurs at 70-80 km and 1000°C within subducting Indian crust. Dense eclogites founder, while buoyant lithologies accumulate in thickening crust. Mantle return flow and radioactive heating in thick, felsic crust maintains high T. The crustal volume in this region grows at 760 m3/yr/m of strike length. Moho-depth earthquakes may be due to localized deformation and thermal runaway in weak layers and diapir margins.

46 CFR 160.060-8 - Marking.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-8 Marking. (a) Each buoyant vest must have the following information clearly... manufacturer or distributor). (Lot No.). (b) Waterproof marking. Marking of buoyant vests shall be sufficiently...

46 CFR 160.060-8 - Marking.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-8 Marking. (a) Each buoyant vest must have the following information clearly... manufacturer or distributor). (Lot No.). (b) Waterproof marking. Marking of buoyant vests shall be sufficiently...

46 CFR 160.060-8 - Marking.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-8 Marking. (a) Each buoyant vest must have the following information clearly... manufacturer or distributor). (Lot No.). (b) Waterproof marking. Marking of buoyant vests shall be sufficiently...

46 CFR 160.010-3 - Inflatable buoyant apparatus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant Vessels § 160.010... container accessible to the occupants, or otherwise secured to the apparatus. Duplicate equipment must be provided, for each side of a reversible inflatable buoyant apparatus, if the equipment is not accessible...

46 CFR 160.010-3 - Inflatable buoyant apparatus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant Vessels § 160.010... container accessible to the occupants, or otherwise secured to the apparatus. Duplicate equipment must be provided, for each side of a reversible inflatable buoyant apparatus, if the equipment is not accessible...

46 CFR 160.010-4 - General requirements for buoyant apparatus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 6 2010-10-01 2010-10-01 false General requirements for buoyant apparatus. 160.010-4 Section 160.010-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant...

46 CFR 160.010-4 - General requirements for buoyant apparatus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 6 2011-10-01 2011-10-01 false General requirements for buoyant apparatus. 160.010-4 Section 160.010-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant...

Engineering zonal cartilage through bioprinting collagen type II hydrogel constructs with biomimetic chondrocyte density gradient.

PubMed

Ren, Xiang; Wang, Fuyou; Chen, Cheng; Gong, Xiaoyuan; Yin, Li; Yang, Liu

2016-07-20

Cartilage tissue engineering is a promising approach for repairing and regenerating cartilage tissue. To date, attempts have been made to construct zonal cartilage that mimics the cartilaginous matrix in different zones. However, little attention has been paid to the chondrocyte density gradient within the articular cartilage. We hypothesized that the chondrocyte density gradient plays an important role in forming the zonal distribution of extracellular matrix (ECM). In this study, collagen type II hydrogel/chondrocyte constructs were fabricated using a bioprinter. Three groups were created according to the total cell seeding density in collagen type II pre-gel: Group A, 2 × 10(7) cells/mL; Group B, 1 × 10(7) cells/mL; and Group C, 0.5 × 10(7) cells/mL. Each group included two types of construct: one with a biomimetic chondrocyte density gradient and the other with a single cell density. The constructs were cultured in vitro and harvested at 0, 1, 2, and 3 weeks for cell viability testing, reverse-transcription quantitative PCR (RT-qPCR), biochemical assays, and histological analysis. We found that total ECM production was positively correlated with the total cell density in the early culture stage, that the cell density gradient distribution resulted in a gradient distribution of ECM, and that the chondrocytes' biosynthetic ability was affected by both the total cell density and the cell distribution pattern. Our results suggested that zonal engineered cartilage could be fabricated by bioprinting collagen type II hydrogel constructs with a biomimetic cell density gradient. Both the total cell density and the cell distribution pattern should be optimized to achieve synergistic biological effects.

In vitro cell-mediated immunity after thermal injury is not impaired. Density gradient purification of mononuclear cells is associated with spurious (artifactual) immunosuppression.

PubMed Central

Xu, D Z; Deitch, E A; Sittig, K; Qi, L; McDonald, J C

1988-01-01

Mononuclear cells isolated by density gradient centrifugation from the peripheral blood of burn patients, but not healthy volunteers, are contaminated with large numbers of nonmononuclear cells. These contaminating leukocytes could cause artifactual alterations in standard in vitro tests of lymphocyte function. Thus, we compared the in vitro blastogenic response of density gradient purified leukocytes and T-cell purified lymphocytes from 13 burn patients to mitogenic (PHA) and antigenic stimuli. The mitogenic and antigenic response of the patients' density gradient purified leukocytes were impaired compared to healthy volunteers (p less than 0.01). However, when the contaminating nonlymphocytes were removed, the patients' cells responded normally to both stimuli. Thus, density gradient purified mononuclear cells from burn patients are contaminated by leukocytes that are not phenotypically or functionally lymphocytes. Since the lymphocytes from burn patients respond normally to PHA and alloantigens after the contaminating nonlymphocyte cell population has been removed, it appears that in vitro assays of lymphocyte function using density gradient purified leukocytes may give spurious results. PMID:2973771

Survival suit volume reduction associated with immersion: implications for buoyancy estimation in offshore workers of different size.

PubMed

Stewart, Arthur; Ledingham, Robert; Furnace, Graham; Williams, Hector; Coleshaw, Susan

2017-06-01

It is currently unknown how body size affects buoyancy in submerged helicopter escape. Eight healthy males aged 39.6 ± 12.6 year (mean ± SD) with BMI 22.0-40.0 kg m -2 wearing a standard survival ('dry') suit undertook a normal venting manoeuvre and underwent 3D scanning to assess body volume (wearing the suit) before and after immersion in a swimming pool. Immersion-induced volume loss averaged 14.4 ± 5.4 l, decreased with increasing dry density (mass volume -1 ) and theoretical buoyant force in 588 UK offshore workers was found to be 264 ± 46 and 232 ± 60 N using linear and power functions, respectively. Both approaches revealed heavier workers to have greater buoyant force. While a larger sample may yield a more accurate buoyancy prediction, this study shows heavier workers are likely to have greater buoyancy. Without free-swimming capability to overcome such buoyancy, some individuals may possibly exceed the safe limit to enable escape from a submerged helicopter. Practitioner Summary: Air expulsion reduced total body volume of survival-suited volunteers following immersion by an amount inversely proportional to body size. When applied to 588 offshore workers, the predicted air loss suggested buoyant force to be greatest in the heaviest individuals, which may impede their ability to exit a submerged helicopter.

A simple technique for measuring buoyant weight increment of entire, transplanted coral colonies in the field.

PubMed

Herler, Jürgen; Dirnwöber, Markus

2011-10-31

Estimating the impacts of global and local threats on coral reefs requires monitoring reef health and measuring coral growth and calcification rates at different time scales. This has traditionally been mostly performed in short-term experimental studies in which coral fragments were grown in the laboratory or in the field but measured ex situ. Practical techniques in which growth and measurements are performed over the long term in situ are rare. Apart from photographic approaches, weight increment measurements have also been applied. Past buoyant weight measurements under water involved a complicated and little-used apparatus. We introduce a new method that combines previous field and laboratory techniques to measure the buoyant weight of entire, transplanted corals under water. This method uses an electronic balance fitted into an acrylic glass underwater housing and placed atop of an acrylic glass cube. Within this cube, corals transplanted onto artificial bases can be attached to the balance and weighed at predetermined intervals while they continue growth in the field. We also provide a set of simple equations for the volume and weight determinations required to calculate net growth rates. The new technique is highly accurate: low error of weight determinations due to variation of coral density (< 0.08%) and low standard error (< 0.01%) for repeated measurements of the same corals. We outline a transplantation technique for properly preparing corals for such long-term in situ experiments and measurements.

46 CFR 160.047-6 - Marking.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-6 Marking. (a) Each buoyant vest must have the following information clearly... buoyant vests shall be sufficiently waterproof so that after 72 hours submergence in water, it will...

46 CFR 160.047-6 - Marking.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-6 Marking. (a) Each buoyant vest must have the following information clearly... buoyant vests shall be sufficiently waterproof so that after 72 hours submergence in water, it will...

46 CFR 160.047-6 - Marking.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-6 Marking. (a) Each buoyant vest must have the following information clearly... buoyant vests shall be sufficiently waterproof so that after 72 hours submergence in water, it will...

46 CFR 160.010-5 - Buoyant apparatus with plastic foam buoyancy.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 6 2010-10-01 2010-10-01 false Buoyant apparatus with plastic foam buoyancy. 160.010-5 Section 160.010-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant...

46 CFR 160.010-5 - Buoyant apparatus with plastic foam buoyancy.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 6 2011-10-01 2011-10-01 false Buoyant apparatus with plastic foam buoyancy. 160.010-5 Section 160.010-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant...

46 CFR 160.052-5 - Construction-standard vests.

Code of Federal Regulations, 2010 CFR

2010-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam... arranged and distributed so as to provide the flotation characteristics and buoyancy required to hold the...) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as shown on Dwg. 160...

Virus purification by CsCl density gradient using general centrifugation.

PubMed

Nasukawa, Tadahiro; Uchiyama, Jumpei; Taharaguchi, Satoshi; Ota, Sumire; Ujihara, Takako; Matsuzaki, Shigenobu; Murakami, Hironobu; Mizukami, Keijirou; Sakaguchi, Masahiro

2017-11-01

Virus purification by cesium chloride (CsCl) density gradient, which generally requires an expensive ultracentrifuge, is an essential technique in virology. Here, we optimized virus purification by CsCl density gradient using general centrifugation (40,000 × g, 2 h, 4 °C), which showed almost the same purification ability as conventional CsCl density gradient ultracentrifugation (100,000 × g, 1 h, 4 °C) using phages S13' and φEF24C. Moreover, adenovirus strain JM1/1 was also successfully purified by this method. We suggest that general centrifugation can become a less costly alternative to ultracentrifugation for virus purification by CsCl densiy gradient and will thus encourage research in virology.

Complex Convective Thermal Fluxes and Vorticity Structure

NASA Astrophysics Data System (ADS)

Redondo, Jose M.; Tellez, Jackson; Sotillos, Laura; Lopez Gonzalez-Nieto, Pilar; Sanchez, Jesus M.; Furmanek, Petr; Diez, Margarita

2015-04-01

Local Diffusion and the topological structure of vorticity and velocity fields is measured in the transition from a homogeneous linearly stratified fluid to a cellular or layered structure by means of convective cooling and/or heating[1,2]. Patterns arise by setting up a convective flow generated by an array of Thermoelectric devices (Peltier/Seebeck cells) these are controlled by thermal PID generating a buoyant heat flux [2]. The experiments described here investigate high Prandtl number mixing using brine and fresh water in order to form density interfaces and low Prandtl number mixing with temperature gradients. The set of dimensionless parameters define conditions of numeric and small scale laboratory modeling of environmental flows. Fields of velocity, density and their gradients were computed and visualized [3,4]. When convective heating and cooling takes place the combination of internal waves and buoyant turbulence is much more complicated if the Rayleigh and Reynolds numbers are high in order to study entrainment and mixing. Using ESS and selfsimilarity structures in the velocity and vorticity fieds and intermittency [3,5] that forms in the non-homogeneous flow is related to mixing and stiring. The evolution of the mixing fronts are compared and the topological characteristics of the merging of plumes and jets in different configurations presenting detailed comparison of the evolution of RM and RT, Jets and Plumes in overall mixing. The relation between structure functions, fractal analysis and spectral analysis can be very useful to determine the evolution of scales. Experimental and numerical results on the advance of a mixing or nonmixing front occurring at a density interface due to body forces [6]and gravitational acceleration are analyzed considering the fractal and spectral structure of the fronts like in removable plate experiments for Rayleigh-Taylor flows. The evolution of the turbulent mixing layer and its complex configuration is studied taking into account the dependence on the initial modes at the early stages and its spectral, self-similar information [3,7-9]. Spectral and Fractal analysis on the images has been used in order to estimate dominant mixing structures as well as the dispersion relations of basic instabilities [4,8. Comparison of the range of entrainment values from laboratory experiments with those ocurring in nature, both in the atmosphere and ocean or in Astrophysics shows the importance of modeling correctly the integral lengthscales of the turbulence. The Entrainment may actually be related to the ratio of the flux to gradient Richardson numbers as well as the Turbulent Schmidt or Prandtl number [6,8] and their structure functions [5]. Turbulent mixing diagnostics are based on schlieren and shadowgraph visualization, planar laser sheet, laser Doppler velocimetry (LDV) and particle image velocimetry (PIV). We are interested by the influence of initial conditions [9](flux gradients of various sizes) on the transition to turbulent mixing, the influence of subsequent vortices and waves, initially in a one dimensional or plane configuration and furthermore in a two-three dimensional configuration with the interface oblique with respect to the horizontal. [1] Redondo J.M. (1992) Termodinámica de los procesos irreversibles, efectos termoeléctricos Rev. Termoelectricidad 2, 16-29.AIT.Pamplona. [2] Redondo J.M., Sanchez J.M. Pascual I. Noriega,G.F.(1995).Thermoelectric regulation for electric cabinets. XVI International Conference in Thermoelectrics, 02-G20. Ed. V. Vedernikov. 456-468. St. Petersburg, Russia. [3] Nicolleau, F.C.G.A.; Cambon, C.; Redondo, J.M.; Vassilicos, J.C.; Reeks, M.; Nowakowski, A.F. (Eds.)(2012) New Approaches in Modeling Multiphase Flows and Dispersion in Turbulence, Fractal Methods and Synthetic Turbulence. ERCOFTAC Series. [4] Redondo J M, Sanchez M A and Cantalapiedra I R (1995). Turbulent Mechanisms in Stratified Flows, Dynamics of Atmospheres and Oceans, 23, 454-462. [5] Mahjoub O.B., Redondo J.M. and Babiano A.(2000) Hyerarchy flux in nonhomogeneous flows in Turbulent diffusion in the environment Eds. Redondo J.M. and Babiano A. 249-260. [6] Matulka A., Redondo J.M. and Carrillo A.(2008) Experiments in stratified and rotating decaying 2D flows, Il Nuovo Cimento 31, 5-6, 757-770. 2008. [7]Fraunie P., Berreba S. Chashechkin Yu.D., Velasco D. and Redondo J.M. (2008) Large eddy simulation and laboratory experiments on the decay of grid wakes in strongly stratified flows. Il Nuovo Cimento C 31, 909-930. [8]Matulka, A., López, P., Redondo, J. M., and Tarquis, A.(2014) On the entrainment coefficient in a forced plume: quantitative effects of source parameters, Nonlin. Processes Geophys., 21, 269-278. [9] Castilla R., Oñate E. and Redondo J.M. (2007) Models, Experiments and Computations in Turbulence. CIMNE, Barcelona, 255.

Loss of plasmid-mediated resistance after conversion of a group B streptococcus strain to a stable cell wall deficient variant.

PubMed

Schmitt-Slomska, J; Caravano, R; El-Solh, N

1979-01-01

A group B streptococcus strain carrying plasmid DNA determining resistance to several drugs was converted by penicillin to cell wall (CW) defective and then to CW deficient variants (L-forms). The stable CW deficient variants became susceptible to antibiotics in study. Dye-buoyant density analysis of the DNA of CW deficient variants showed that the loss of antibiotic resistance was associated with the loss of extrachromosomal DNA.

Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions

PubMed Central

Noguchi, Mana; Kurisu, Futoshi; Kasuga, Ikuro; Furumai, Hiroaki

2014-01-01

To identify the microorganisms involved in benzene degradation, DNA-stable isotope probing (SIP) with 13C-benzene was applied to a methanogenic benzene-degrading enrichment culture. Pyrosequencing of ribosomal RNA (rRNA) gene sequences revealed that the community structure was highly complex in spite of a 3-year incubation only with benzene. The culture degraded 98% of approximately 1 mM 13C-benzene and mineralized 72% of that within 63 d. The terminal restriction fragment length polymorphism (T-RFLP) profiles of the buoyant density fractions revealed the incorporation of 13C into two phylotypes after 64 d. These two phylotypes were determined to be Desulfobacterales- and Coriobacteriaceae-related bacteria by cloning and sequencing of the 16S rRNA gene in the 13C-labeled DNA abundant fraction. Comparative pyrosequencing analysis of the buoyant density fractions of 12C- and 13C-labeled samples indicated the incorporation of 13C into three bacterial and one archaeal OTUs related to Desulfobacterales, Coriobacteriales, Rhodocyclaceae, and Methanosarcinales. The first two OTUs included the bacteria detected by T-RFLP-cloning-sequencing analysis. Furthermore, time-resolved SIP analysis confirmed that the activity of all these microbes appeared at the earliest stage of degradation. In this methanogenic culture, Desulfobacterales- and Coriobacteriaceae-related bacteria were most likely to be the major benzene degraders. PMID:24909708

46 CFR 28.130 - Survival craft equipment.

Code of Federal Regulations, 2014 CFR

2014-10-01

... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

46 CFR 28.130 - Survival craft equipment.

Code of Federal Regulations, 2012 CFR

2012-10-01

... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

46 CFR 28.130 - Survival craft equipment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

46 CFR 28.130 - Survival craft equipment.

Code of Federal Regulations, 2013 CFR

2013-10-01

... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

46 CFR 28.130 - Survival craft equipment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

46 CFR 160.047-5 - Inspections and tests. 1

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult... labeled buoyant vests shall— (1) Maintain quality control of the materials used, the manufacturing methods.... (b) Lot size and sampling. (1) A lot consists of 500 buoyant vests or fewer. (2) A new lot begins...

46 CFR 160.047-3 - Materials.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-3 Materials. (a) General. All components used in the construction of buoyant vests... requirements of specification MIL-B-2766. (d) Envelope. The buoyant vest envelope, or cover, shall be made from...

46 CFR 160.052-2 - Size and model.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-2 Size and model. (a) A standard buoyant vest is manufactured in accordance... (for persons weighing less than 50 pounds). (b) A nonstandard buoyant vest is: (1) Manufactured in...

46 CFR 160.047-5 - Inspections and tests. 1

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult... labeled buoyant vests shall— (1) Maintain quality control of the materials used, the manufacturing methods.... (b) Lot size and sampling. (1) A lot consists of 500 buoyant vests or fewer. (2) A new lot begins...

46 CFR 160.047-3 - Materials.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-3 Materials. (a) General. All components used in the construction of buoyant vests... requirements of specification MIL-B-2766. (d) Envelope. The buoyant vest envelope, or cover, shall be made from...

46 CFR 160.052-2 - Size and model.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-2 Size and model. (a) A standard buoyant vest is manufactured in accordance... (for persons weighing less than 50 pounds). (b) A nonstandard buoyant vest is: (1) Manufactured in...

46 CFR 160.047-5 - Inspections and tests. 1

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult... labeled buoyant vests shall— (1) Maintain quality control of the materials used, the manufacturing methods.... (b) Lot size and sampling. (1) A lot consists of 500 buoyant vests or fewer. (2) A new lot begins...

46 CFR 160.052-2 - Size and model.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-2 Size and model. (a) A standard buoyant vest is manufactured in accordance... (for persons weighing less than 50 pounds). (b) A nonstandard buoyant vest is: (1) Manufactured in...

46 CFR 160.052-2 - Size and model.

Code of Federal Regulations, 2011 CFR

2011-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-2 Size and model. (a) A standard buoyant vest is manufactured in accordance... (for persons weighing less than 50 pounds). (b) A nonstandard buoyant vest is: (1) Manufactured in...

46 CFR 160.047-3 - Materials.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-3 Materials. (a) General. All components used in the construction of buoyant vests... requirements of specification MIL-B-2766. (d) Envelope. The buoyant vest envelope, or cover, shall be made from...

46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

Code of Federal Regulations, 2012 CFR

2012-10-01

... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST..., and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced... maintenance of inflatable rescue boats must follow the manufacturers' instructions. Each repair, except an...

46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

Code of Federal Regulations, 2013 CFR

2013-10-01

... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST..., and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced... maintenance of inflatable rescue boats must follow the manufacturers' instructions. Each repair, except an...

46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

Code of Federal Regulations, 2014 CFR

2014-10-01

... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST..., and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced... maintenance of inflatable rescue boats must follow the manufacturers' instructions. Each repair, except an...

The effect of particle properties on the depth profile of buoyant plastics in the ocean

NASA Astrophysics Data System (ADS)

Kooi, Merel; Reisser, Julia; Slat, Boyan; Ferrari, Francesco F.; Schmid, Moritz S.; Cunsolo, Serena; Brambini, Roberto; Noble, Kimberly; Sirks, Lys-Anne; Linders, Theo E. W.; Schoeneich-Argent, Rosanna I.; Koelmans, Albert A.

2016-10-01

Most studies on buoyant microplastics in the marine environment rely on sea surface sampling. Consequently, microplastic amounts can be underestimated, as turbulence leads to vertical mixing. Models that correct for vertical mixing are based on limited data. In this study we report measurements of the depth profile of buoyant microplastics in the North Atlantic subtropical gyre, from 0 to 5 m depth. Microplastics were separated into size classes (0.5-1.5 and 1.5-5.0 mm) and types (‘fragments’ and ‘lines’), and associated with a sea state. Microplastic concentrations decreased exponentially with depth, with both sea state and particle properties affecting the steepness of the decrease. Concentrations approached zero within 5 m depth, indicating that most buoyant microplastics are present on or near the surface. Plastic rise velocities were also measured, and were found to differ significantly for different sizes and shapes. Our results suggest that (1) surface samplers such as manta trawls underestimate total buoyant microplastic amounts by a factor of 1.04-30.0 and (2) estimations of depth-integrated buoyant plastic concentrations should be done across different particle sizes and types. Our findings can assist with improving buoyant ocean plastic vertical mixing models, mass balance exercises, impact assessments and mitigation strategies.

The effect of particle properties on the depth profile of buoyant plastics in the ocean

PubMed Central

Kooi, Merel; Reisser, Julia; Slat, Boyan; Ferrari, Francesco F.; Schmid, Moritz S.; Cunsolo, Serena; Brambini, Roberto; Noble, Kimberly; Sirks, Lys-Anne; Linders, Theo E. W.; Schoeneich-Argent, Rosanna I.; Koelmans, Albert A.

2016-01-01

Most studies on buoyant microplastics in the marine environment rely on sea surface sampling. Consequently, microplastic amounts can be underestimated, as turbulence leads to vertical mixing. Models that correct for vertical mixing are based on limited data. In this study we report measurements of the depth profile of buoyant microplastics in the North Atlantic subtropical gyre, from 0 to 5 m depth. Microplastics were separated into size classes (0.5–1.5 and 1.5–5.0 mm) and types (‘fragments’ and ‘lines’), and associated with a sea state. Microplastic concentrations decreased exponentially with depth, with both sea state and particle properties affecting the steepness of the decrease. Concentrations approached zero within 5 m depth, indicating that most buoyant microplastics are present on or near the surface. Plastic rise velocities were also measured, and were found to differ significantly for different sizes and shapes. Our results suggest that (1) surface samplers such as manta trawls underestimate total buoyant microplastic amounts by a factor of 1.04–30.0 and (2) estimations of depth-integrated buoyant plastic concentrations should be done across different particle sizes and types. Our findings can assist with improving buoyant ocean plastic vertical mixing models, mass balance exercises, impact assessments and mitigation strategies. PMID:27721460

The effect of particle properties on the depth profile of buoyant plastics in the ocean.

PubMed

Kooi, Merel; Reisser, Julia; Slat, Boyan; Ferrari, Francesco F; Schmid, Moritz S; Cunsolo, Serena; Brambini, Roberto; Noble, Kimberly; Sirks, Lys-Anne; Linders, Theo E W; Schoeneich-Argent, Rosanna I; Koelmans, Albert A

2016-10-10

Most studies on buoyant microplastics in the marine environment rely on sea surface sampling. Consequently, microplastic amounts can be underestimated, as turbulence leads to vertical mixing. Models that correct for vertical mixing are based on limited data. In this study we report measurements of the depth profile of buoyant microplastics in the North Atlantic subtropical gyre, from 0 to 5 m depth. Microplastics were separated into size classes (0.5-1.5 and 1.5-5.0 mm) and types ('fragments' and 'lines'), and associated with a sea state. Microplastic concentrations decreased exponentially with depth, with both sea state and particle properties affecting the steepness of the decrease. Concentrations approached zero within 5 m depth, indicating that most buoyant microplastics are present on or near the surface. Plastic rise velocities were also measured, and were found to differ significantly for different sizes and shapes. Our results suggest that (1) surface samplers such as manta trawls underestimate total buoyant microplastic amounts by a factor of 1.04-30.0 and (2) estimations of depth-integrated buoyant plastic concentrations should be done across different particle sizes and types. Our findings can assist with improving buoyant ocean plastic vertical mixing models, mass balance exercises, impact assessments and mitigation strategies.

Metabolic alterations, HFE gene mutations and atherogenic lipoprotein modifications in patients with primary iron overload.

PubMed

Meroño, Tomás; Brites, Fernando; Dauteuille, Carolane; Lhomme, Marie; Menafra, Martín; Arteaga, Alejandra; Castro, Marcelo; Saez, María Soledad; Ballerga, Esteban González; Sorroche, Patricia; Rey, Jorge; Lesnik, Philippe; Sordá, Juan Andrés; Chapman, M John; Kontush, Anatol; Daruich, Jorge

2015-05-01

Iron overload (IO) has been associated with glucose metabolism alterations and increased risk of cardiovascular disease (CVD). Primary IO is associated with mutations in the HFE gene. To which extent HFE gene mutations and metabolic alterations contribute to the presence of atherogenic lipoprotein modifications in primary IO remains undetermined. The present study aimed to assess small, dense low-density lipoprotein (LDL) levels, chemical composition of LDL and high-density lipoprotein (HDL) particles, and HDL functionality in IO patients. Eighteen male patients with primary IO and 16 sex- and age-matched controls were recruited. HFE mutations (C282Y, H63D and S65C), measures of insulin sensitivity and secretion (calculated from the oral glucose tolerance test), chemical composition and distribution profile of LDL and HDL subfractions (isolated by gradient density ultracentrifugation) and HDL functionality (as cholesterol efflux and antioxidative activity) were studied. IO patients compared with controls exhibited insulin resistance (HOMA-IR (homoeostasis model assessment-estimated insulin resistance): +93%, P< 0.001). Metabolic profiles differed across HFE genotypes. C282Y homozygotes (n=7) presented a reduced β-cell function and insulin secretion compared with non-C282Y patients (n=11) (-58% and -73%, respectively, P< 0.05). In addition, C282Y homozygotes featured a predominance of large, buoyant LDL particles (C282Y: 43±5; non-C282Y: 25±8; controls: 32±7%; P< 0.001), whereas non-C282Y patients presented higher amounts of small, dense LDL (C282Y: 23±5; non-C282Y: 39±10; controls: 26±4%; P< 0.01). HDL particles were altered in C282Y homozygotes. However, HDL functionality was conserved. In conclusion, metabolic alterations and HFE gene mutations are involved in the presence of atherogenic lipoprotein modifications in primary IO. To what extent such alterations could account for an increase in CVD risk remains to be determined.

Impact of the Extreme Warming of 2012 on Shelfbreak Frontal Structure North of Cape Hatteras

NASA Astrophysics Data System (ADS)

Gawarkiewickz, G.

2014-12-01

Continental shelf circulation north of Cape Hatteras is complex, with southward flowing Middle Atlantic Bight shelf water intersecting the Gulf Stream and subducting offshore into the Gulf Stream. In May, 2012, a cruise was conducted in order to study the shelf circulation and acoustic propagation through fish schools in the area. An important aspect of the study was to use Autonomous Underwater Vehicles to map fish schools with a sidescan sonar. High-resolution hydrographic surveys to map the continental shelf water masses and shelfbreak frontal structure were sampled to relate oceanographic conditions to the fish school distributions. The cold pool water mass over the continental shelf in May 2012 was extremely warm, with temperature anomalies of up to 5 Degrees C relative to observations from the same area in May, 1996. The normal cross-shelf temperature gradients within the shelfbreak front were not present because of the warming. As a result, the shelf density field was much more buoyant than usual, which led to an accelerated shelfbreak jet. Moored velocity measurements at the 60 m isobath recorded alongshelf flow of as much as 0.6 m/s. The anticipated fish species were not observed over the continental shelf. Some comments on the forcing leading to the large scale warming will be presented, along with a brief discussion of the impact of the warming on the marine ecosystem in the northeast U.S.

Use of Total Electron Content data to analyze ionosphere electron density gradients

NASA Astrophysics Data System (ADS)

Nava, B.; Radicella, S. M.; Leitinger, R.; Coisson, P.

In presence of electron density gradients the thin shell approximation for the ionosphere used together with a simple mapping function to convert slant Total Electron Content TEC to vertical TEC could lead to TEC conversion errors Therefore these mapping function errors can be used to identify the effects of the electron density gradients in the ionosphere In the present work high precision GPS derived slant TEC data have been used to investigate the effects of the electron density gradients in the middle and low latitude ionosphere under geomagnetic quiet and disturbed conditions In particular the data corresponding to the geographic area of the American sector for the days 5-7 April 2000 have been used to perform a complete analysis of mapping function errors based on the coinciding pierce point technique The results clearly illustrate the electron density gradient effects according to the locations considered and to the actual levels of disturbance of the ionosphere

First-row diatomics: Calculation of the geometry and energetics using self-consistent gradient-functional approximations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kutzler, F.W.; Painter, G.S.

1992-02-15

A fully self-consistent series of nonlocal (gradient) density-functional calculations has been carried out using the augmented-Gaussian-orbital method to determine the magnitude of gradient corrections to the potential-energy curves of the first-row diatomics, Li{sub 2} through F{sub 2}. Both the Langreth-Mehl-Hu and the Perdew-Wang gradient-density functionals were used in calculations of the binding energy, bond length, and vibrational frequency for each dimer. Comparison with results obtained in the local-spin-density approximation (LSDA) using the Vosko-Wilk-Nusair functional, and with experiment, reveals that bond lengths and vibrational frequencies are rather insensitive to details of the gradient functionals, including self-consistency effects, but the gradient correctionsmore » reduce the overbinding commonly observed in the LSDA calculations of first-row diatomics (with the exception of Li{sub 2}, the gradient-functional binding-energy error is only 50--12 % of the LSDA error). The improved binding energies result from a large differential energy lowering, which occurs in open-shell atoms relative to the diatomics. The stabilization of the atom arises from the use of nonspherical charge and spin densities in the gradient-functional calculations. This stabilization is negligibly small in LSDA calculations performed with nonspherical densities.« less

46 CFR 160.052-5 - Construction-standard vests.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as shown on Dwg. 160...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam.... Two pieces of fabric shall be cut to the pattern shown on Dwg. No. 160.052-1, Sheet 1 for adult size...

46 CFR 160.052-5 - Construction-standard vests.

Code of Federal Regulations, 2012 CFR

2012-10-01

...) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as shown on Dwg. 160...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam.... Two pieces of fabric shall be cut to the pattern shown on Dwg. No. 160.052-1, Sheet 1 for adult size...

46 CFR 160.052-5 - Construction-standard vests.

Code of Federal Regulations, 2014 CFR

2014-10-01

...) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as shown on Dwg. 160...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam.... Two pieces of fabric shall be cut to the pattern shown on Dwg. No. 160.052-1, Sheet 1 for adult size...

46 CFR 160.052-5 - Construction-standard vests.

Code of Federal Regulations, 2013 CFR

2013-10-01

...) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as shown on Dwg. 160...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam.... Two pieces of fabric shall be cut to the pattern shown on Dwg. No. 160.052-1, Sheet 1 for adult size...

46 CFR 180.137 - Stowage of life floats and buoyant apparatus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Stowage of life floats and buoyant apparatus. 180.137... (UNDER 100 GROSS TONS) LIFESAVING EQUIPMENT AND ARRANGEMENTS Survival Craft Arrangements and Equipment § 180.137 Stowage of life floats and buoyant apparatus. (a) In addition to meeting § 180.130, each life...

46 CFR 180.137 - Stowage of life floats and buoyant apparatus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Stowage of life floats and buoyant apparatus. 180.137... (UNDER 100 GROSS TONS) LIFESAVING EQUIPMENT AND ARRANGEMENTS Survival Craft Arrangements and Equipment § 180.137 Stowage of life floats and buoyant apparatus. (a) In addition to meeting § 180.130, each life...

46 CFR 160.010-6 - Capacity of buoyant apparatus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant Vessels § 160.010... immersed or where facilities are provided for climbing on top of the apparatus. (2) Number of 300 mm (1 ft... 46 Shipping 6 2011-10-01 2011-10-01 false Capacity of buoyant apparatus. 160.010-6 Section 160.010...

46 CFR 160.010-6 - Capacity of buoyant apparatus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant Vessels § 160.010... immersed or where facilities are provided for climbing on top of the apparatus. (2) Number of 300 mm (1 ft... 46 Shipping 6 2010-10-01 2010-10-01 false Capacity of buoyant apparatus. 160.010-6 Section 160.010...

Particle simulation of electromagnetic emissions from electrostatic instability driven by an electron ring beam on the density gradient

NASA Astrophysics Data System (ADS)

Horký, Miroslav; Omura, Yoshiharu; Santolík, Ondřej

2018-04-01

This paper presents the wave mode conversion between electrostatic and electromagnetic waves on the plasma density gradient. We use 2-D electromagnetic code KEMPO2 implemented with the generation of density gradient to simulate such a conversion process. In the dense region, we use ring beam instability to generate electron Bernstein waves and we study the temporal evolution of wave spectra, velocity distributions, Poynting flux, and electric and magnetic energies to observe the wave mode conversion. Such a conversion process can be a source of electromagnetic emissions which are routinely measured by spacecraft on the plasmapause density gradient.

Density-Gradient-Driven trapped-electron-modes in improved-confinement RFP plasmas

NASA Astrophysics Data System (ADS)

Duff, James; Sarff, John; Ding, Weixing; Brower, David; Parke, Eli; Chapman, Brett; Terry, Paul; Pueschel, M. J.; Williams, Zach

2017-10-01

Short wavelength density fluctuations in improved-confinement MST plasmas exhibit multiple features characteristic of the trapped-electron-mode (TEM). Core transport in the RFP is normally governed by magnetic stochasticity stemming from long wavelength tearing modes that arise from current profile peaking, which are suppressed via inductive control for this work. The improved confinement is associated with an increase in the pressure gradient that can destabilize drift waves. The measured density fluctuations have f 50 kHz, kϕρs < 0.14 , and propagate in the electron drift direction. Their spectral emergence coincides with a sharp decrease in global tearing mode associated fluctuations, their amplitude increases with local density gradient, and they exhibit a density-gradient threshold at R /Ln 15 . The GENE code, modified for the RFP, predicts the onset of density-gradient-driven TEM for these strong-gradient plasma conditions. While nonlinear analysis shows a large Dimits shift associated with predicted strong zonal flows, the inclusion of residual magnetic fluctuations, comparable to experimental magnetic fluctuations, causes a collapse of the zonal flows and an increase in the predicted transport to a level close to the experimentally measured heat flux. Work supported by US DOE.

Experimental Study of Buoyant-Thermocapillary Convection in a Rectangular Cavity

NASA Technical Reports Server (NTRS)

Braunsfurth, Manfred G.; Homsy, George M.

1996-01-01

The problem of buoyant-thermocapillary convection in cavities is governed by a relatively large number of nondimensional parameters, and there is consequently a large number of different types of flow that can be found in this system. Previous results give disjoint glimpses of a wide variety of qualitatively and quantitatively different results in widely different parts of parameter space. In this study, we report experiments on the primary and secondary instabilities in a geometry with equal aspect ratios in the range from 1 to 8 in both the direction along and perpendicular to the applied temperature gradient. We thus complement previous work which mostly involved either fluid layers of large extent in both directions, or consisted of investigations of strictly two-dimensional disturbances. We observe the primary transition from an essentially two-dimensional flow to steady three-dimensional longitudinal rolls. The critical Marangoni number is found to depend on the aspect ratios of the system, and varies from 4.6 x 10(exp 5) at aspect ratio 2.0 to 5.5 x 10(exp 4) at aspect ratio 3.5. Further, we have investigated the stability of the three-dimensional flow at larger Marangoni numbers, and find a novel oscillatory flow at critical Marangoni numbers of the order of 6 x 10(exp 5). We suggest possible mechanisms which give rise to the oscillation, and find that it is expected to be a relaxation type oscillation.

The effect of density gradients on hydrometers

NASA Astrophysics Data System (ADS)

Heinonen, Martti; Sillanpää, Sampo

2003-05-01

Hydrometers are simple but effective instruments for measuring the density of liquids. In this work, we studied the effect of non-uniform density of liquid on a hydrometer reading. The effect induced by vertical temperature gradients was investigated theoretically and experimentally. A method for compensating for the effect mathematically was developed and tested with experimental data obtained with the MIKES hydrometer calibration system. In the tests, the method was found reliable. However, the reliability depends on the available information on the hydrometer dimensions and density gradients.

Molecular Markers for Breast Cancer Susceptibility.

DTIC Science & Technology

1997-09-01

Cells were harvested after 24hrs and stored at -80’C until analyzed. Preparation and analysis of RNA The fourth abdominal mammary glands from virgin...at -80’C. Total cellular RNA was prepared using 4M guanidium isothiocynate and CsCl buoyant density centrifugation (Maniatis, 1988). RNA was...were identified. The RNA expression levels of EDD-C3, C6, C11, C14, C15, C18, G5, G6 and G7 were analyzed by RT-PCR in an attempt to identify clones

Density-Gradient-Driven trapped-electron-modes in improved-confinement RFP plasmas

NASA Astrophysics Data System (ADS)

Duff, James

2016-10-01

Short wavelength density fluctuations in improved-confinement MST plasmas exhibit multiple features characteristic of the trapped-electron-mode (TEM), strong evidence that drift wave turbulence emerges in RFP plasmas when transport associated with MHD tearing is reduced. Core transport in the RFP is normally governed by magnetic stochasticity stemming from long wavelength tearing modes that arise from current profile peaking. Using inductive control, the tearing modes are reduced and global confinement is increased to values expected for a comparable tokamak plasma. The improved confinement is associated with a large increase in the pressure gradient that can destabilize drift waves. The measured density fluctuations have frequencies >50 kHz, wavenumbers k_phi*rho_s<0.14, and propagate in the electron drift direction. Their spectral emergence coincides with a sharp decrease in fluctuations associated with global tearing modes. Their amplitude increases with the local density gradient, and they exhibit a density-gradient threshold at R/L_n 15, higher than in tokamak plasmas by R/a. the GENE code, modified for RFP equilibria, predicts the onset of microinstability for these strong-gradient plasma conditions. The density-gradient-driven TEM is the dominant instability in the region where the measured density fluctuations are largest, and the experimental threshold-gradient is close to the predicted critical gradient for linear stability. While nonlinear analysis shows a large Dimits shift associated with predicted strong zonal flows, the inclusion of residual magnetic fluctuations causes a collapse of the zonal flows and an increase in the predicted transport to a level close to the experimentally measured heat flux. Similar circumstances could occur in the edge region of tokamak plasmas when resonant magnetic perturbations are applied for the control of ELMs. Work supported by US DOE.

Wind influence on a coastal buoyant outflow

NASA Astrophysics Data System (ADS)

Whitney, Michael M.; Garvine, Richard W.

2005-03-01

This paper investigates the interplay between river discharge and winds in forcing coastal buoyant outflows. During light winds a plume influenced by the Earth's rotation will flow down shelf (in the direction of Kelvin wave propagation) as a slender buoyancy-driven coastal current. Downwelling favorable winds augment this down-shelf flow, narrow the plume, and mix the water column. Upwelling favorable winds drive currents that counter the buoyancy-driven flow, spread plume waters offshore, and rapidly mix buoyant waters. Two criteria are developed to assess the wind influence on a buoyant outflow. The wind strength index (Ws) determines whether a plume's along-shelf flow is in a wind-driven or buoyancy-driven state. Ws is the ratio of the wind-driven and buoyancy-driven along-shelf velocities. Wind influence on across-shelf plume structure is rated with a timescale (ttilt) for the isopycnal tilting caused by wind-driven Ekman circulation. These criteria are used to characterize wind influence on the Delaware Coastal Current and can be applied to other coastal buoyant outflows. The Delaware buoyant outflow is simulated for springtime high-river discharge conditions. Simulation results and Ws values reveal that the coastal current is buoyancy-driven most of the time (∣Ws∣ < 1 on average). Wind events, however, overwhelm the buoyancy-driven flow (∣Ws∣ > 1) several times during the high-discharge period. Strong upwelling events reverse the buoyant outflow; they constitute an important mechanism for transporting fresh water up shelf. Across-shelf plume structure is more sensitive to wind influence than the along-shelf flow. Values of ttilt indicate that moderate or strong winds persisting throughout a day can modify plume width significantly. Plume widening during upwelling events is accompanied by mixing that can erase the buoyant outflow.

Vehicle concepts and technology requirements for buoyant heavy-lift systems

NASA Technical Reports Server (NTRS)

Ardema, M. D.

1981-01-01

Several buoyant-vehicle (airship) concepts proposed for short hauls of heavy payloads are described. Numerous studies identified operating cost and payload capacity advantages relative to existing or proposed heavy-lift helicopters for such vehicles. Applications involving payloads of from 15 tons up to 800 tons were identified. The buoyant quad-rotor concept is discussed in detail, including the history of its development, current estimates of performance and economics, currently perceived technology requirements, and recent research and technology development. It is concluded that the buoyant quad-rotor, and possibly other buoyant vehicle concepts, has the potential of satisfying the market for very heavy vertical lift but that additional research and technology development are necessary. Because of uncertainties in analytical prediction methods and small-scale experimental measurements, there is a strong need for large or full-scale experiments in ground test facilities and, ultimately, with a flight research vehicle.

The small dense LDL particle/large buoyant LDL particle ratio is associated with glucose metabolic status in pregnancy.

PubMed

Chen, Yanmin; Du, Mengkai; Xu, Jianyun; Chen, Danqing

2017-12-14

The lipoprotein subfraction particle profile can be used to improve clinical assessments of cardiovascular disease risk and contribute to early detection of atherogenic dyslipidemia. Lipid alterations in gestational diabetes have been extensively studied, but the results have been inconsistent. Here, we investigated serum lipoprotein subfraction particle levels and their association with glucose metabolic status in pregnancy. Twenty-eight pregnant women with gestational diabetes and 56 pregnant women with normal glucose tolerance matched for body mass index were enrolled in this study. We assessed fasting serum lipid concentrations and lipoprotein subfraction particle levels in participants between 24 and 28 weeks of gestation. The level of low-density lipoprotein (LDL) cholesterol was significantly lower in women with gestational diabetes than in those with normal glucose tolerance, but the triglyceride and high-density lipoprotein (HDL) cholesterol levels of the two groups were similar. Lipoprotein particle analysis showed that very-low-density lipoprotein (VLDL) particle number and the small dense LDL particle/large buoyant LDL particle (sdLDL-P/lbLDL-P) ratio were significantly higher in women with gestational diabetes than in those with normal glucose tolerance (P = 0.013 and P = 0.015, respectively). In multivariate analysis, fasting glucose was independently and positively associated with sdLDL-P/lbLDL-P ratio even after adjustment for maternal age, gestational weight gain, BMI and LDL cholesterol (standardized Beta = 0.214, P = 0.029). The sdLDL-P/lbLDL-P ratio is higher in GDM compared with non-diabetic pregnant women, and positively and independently associated with fasting glucose in pregnant women.

Horizontal density-gradient effects on simulation of flow and transport in the Potomac Estuary

USGS Publications Warehouse

Schaffranek, Raymond W.; Baltzer, Robert A.; ,

1990-01-01

A two-dimensional, depth-integrated, hydrodynamic/transport model of the Potomac Estuary between Indian Head and Morgantown, Md., has been extended to include treatment of baroclinic forcing due to horizontal density gradients. The finite-difference model numerically integrates equations of mass and momentum conservation in conjunction with a transport equation for heat, salt, and constituent fluxes. Lateral and longitudinal density gradients are determined from salinity distributions computed from the convection-diffusion equation and an equation of state that expresses density as a function of temperature and salinity; thus, the hydrodynamic and transport computations are directly coupled. Horizontal density variations are shown to contribute significantly to momentum fluxes determined in the hydrodynamic computation. These fluxes lead to enchanced tidal pumping, and consequently greater dispersion, as is evidenced by numerical simulations. Density gradient effects on tidal propagation and transport behavior are discussed and demonstrated.

Use of total electron content data to analyze ionosphere electron density gradients

NASA Astrophysics Data System (ADS)

Nava, B.; Radicella, S. M.; Leitinger, R.; Coïsson, P.

In the presence of electron density gradients the thin shell approximation for the ionosphere, used together with a simple mapping function to convert slant total electron content (TEC) to vertical TEC, could lead to TEC conversion errors. These "mapping function errors" can therefore be used to detect the electron density gradients in the ionosphere. In the present work GPS derived slant TEC data have been used to investigate the effects of the electron density gradients in the middle and low latitude ionosphere under geomagnetic quiet and disturbed conditions. In particular the data corresponding to the geographic area of the American Sector for the days 5-7 April 2000 have been used to perform a complete analysis of mapping function errors based on the "coinciding pierce point technique". The results clearly illustrate the electron density gradient effects according to the locations considered and to the actual levels of disturbance of the ionosphere. In addition, the possibility to assess an ionospheric shell height able to minimize the mapping function errors has been verified.

On some physical and dynamical properties of microplastic particles in marine environment.

PubMed

Chubarenko, I; Bagaev, A; Zobkov, M; Esiukova, E

2016-07-15

Simplified physical models and geometrical considerations reveal general physical and dynamical properties of microplastic particles (0.5-5mm) of different density, shape and size in marine environment. Windage of extremely light foamed particles, surface area and fouling rate of slightly positively buoyant microplastic spheres, films and fibres and settling velocities of negatively buoyant particles are analysed. For the Baltic Sea dimensions and under the considered idealised external conditions, (i) only one day is required for a foamed polystyrene particle to cross the sea (ca. 250km); (ii) polyethylene fibres should spend about 6-8months in the euphotic zone before sinking due to bio-fouling, whilst spherical particles can be retained on the surface up to 10-15years; (iii) for heavy microplastic particles, the time of settling through the water column in the central Gotland basin (ca. 250m) is less than 18h. Proper physical setting of the problem of microplastics transport and developing of physically-based parameterisations are seen as applications. Copyright © 2016 Elsevier Ltd. All rights reserved.

An analysis of a mixed convection associated with thermal heating in contaminated porous media.

PubMed

Krol, Magdalena M; Johnson, Richard L; Sleep, Brent E

2014-11-15

The occurrence of subsurface buoyant flow during thermal remediation was investigated using a two dimensional electro-thermal model (ETM). The model incorporated electrical current flow associated with electrical resistance heating, energy and mass transport, and density dependent water flow. The model was used to examine the effects of heating on sixteen subsurface scenarios with different applied groundwater fluxes and soil permeabilities. The results were analyzed in terms of the ratio of Rayleigh to thermal Peclet numbers (the buoyancy ratio). It was found that when the buoyancy number was greater than unity and the soil permeability greater than 10(-12) m(2), buoyant flow and contaminant transport were significant. The effects of low permeability layers and electrode placement on heat and mass transport were also investigated. Heating under a clay layer led to flow stagnation zones resulting in the accumulation of contaminant mass and transport into the low permeability layer. The results of this study can be used to develop dimensionless number-based guidelines for site management during subsurface thermal activities. Copyright © 2014 Elsevier B.V. All rights reserved.

Experimental Demonstration of Underwater Acoustic Scattering Cancellation

PubMed Central

Rohde, Charles A.; Martin, Theodore P.; Guild, Matthew D.; Layman, Christopher N.; Naify, Christina J.; Nicholas, Michael; Thangawng, Abel L.; Calvo, David C.; Orris, Gregory J.

2015-01-01

We explore an acoustic scattering cancellation shell for buoyant hollow cylinders submersed in a water background. A thin, low-shear, elastic coating is used to cancel the monopole scattering from an air-filled, neutrally buoyant steel shell for all frequencies where the wavelength is larger than the object diameter. By design, the uncoated shell also has an effective density close to the aqueous background, independently canceling its dipole scattering. Due to the significantly reduced monopole and dipole scattering, the compliant coating results in a hollow cylindrical inclusion that is simultaneously impedance and sound speed matched to the water background. We demonstrate the proposed cancellation method with a specific case, using an array of hollow steel cylinders coated with thin silicone rubber shells. These experimental results are matched to finite element modeling predictions, confirming the scattering reduction. Additional calculations explore the optimization of the silicone coating properties. Using this approach, it is found that scattering cross-sections can be reduced by 20 dB for all wavelengths up to k0a = 0.85. PMID:26282067

A layer of neutrally buoyant olivine in the early stages of a deep lunar magma ocean and a possible consecutive overturn

NASA Astrophysics Data System (ADS)

Krättli, G.; Schmidt, M. W.

2017-12-01

The moon is thought to have undergone a completely molten stage during its accretion, the lunar magma ocean. In order to understand the evolution and first differentiation of the lunar magma ocean, we performed a series of consecutive liquidus experiments at pressures of the lower half of the lunar magma ocean. In these experiments, we determined the liquidus, crystallized some amount of minerals (typically 10-20%) and then stepped to a new bulk composition representing the residual liquid after fractionation of these minerals. Mineral and melt densities were then calculated in order to decide whether minerals would float or sink. The bulk lunar composition used in this study (Taylor 1982) results in extensive early olivine crystallization with high XMg (94-90) for all experimental pressures, the liquidus temperature slightly decreasing from 1900 to 1850°C from 4.5 to 3.5 GPa. Crystallization begins at the core-mantle boundary, but calculations indicate that olivine initially floats and becomes neutrally buoyant at 3.5-3.7 GPa, leading to a stable olivine layer of several 100 km thickness at this depth. This layer should rapidly compact yielding two chemically separated magma reservoirs. Olivine crystallization is followed by orthopyroxene (1650°C, twm_fr2), minor garnet (1600°C, twm_fr3), clinopyroxene and spinel (1550°C, twm_fr3) in the lower magma ocean. Despite continuously decreasing XMg and increasing Ca/Al, further experiments indicate that the more extensively fractionated lower magma should become finally buoyant, possibly causing an overturn of the previously layered structure. Additionally, few centrifuge assisted experiments at 2.5-3.5 GPa were performed showing decreasing olivine-melt density contrasts with increasing pressure. Slightly higher pressures would be necessary to positively prove the neutral buoyancy of olivine at 3.6 GPa, currently we are improving the piston cylinder on the centrifuge to reach 4 GPa. Taylor, Stuart Ross. Planetary science: a lunar perspective. Vol. 3303. Houston: Lunar and Planetary Institute, 1982.

The effect of shear flow and the density gradient on the Weibel instability growth rate in the dense plasma

NASA Astrophysics Data System (ADS)

Amininasab, S.; Sadighi-Bonabi, R.; Khodadadi Azadboni, F.

2018-02-01

Shear stress effect has been often neglected in calculation of the Weibel instability growth rate in laser-plasma interactions. In the present work, the role of the shear stress in the Weibel instability growth rate in the dense plasma with density gradient is explored. By increasing the density gradient, the shear stress threshold is increasing and the range of the propagation angles of growing modes is limited. Therefore, by increasing steps of the density gradient plasma near the relativistic electron beam-emitting region, the Weibel instability occurs at a higher stress flow. Calculations show that the minimum value of the stress rate threshold for linear polarization is greater than that of circular polarization. The Wiebel instability growth rate for linear polarization is 18.3 times circular polarization. One sees that for increasing stress and density gradient effects, there are smaller maximal growth rates for the range of the propagation angles of growing modes /π 2 < θ m i n < π and /3 π 2 < θ m i n < 2 π in circular polarized plasma and for /k c ω p < 4 in linear polarized plasma. Therefore, the shear stress and density gradient tend to stabilize the Weibel instability for /k c ω p < 4 in linear polarized plasma. Also, the shear stress and density gradient tend to stabilize the Weibel instability for the range of the propagation angles of growing modes /π 2 < θ m i n < π and /3 π 2 < θ m i n < 2 π in circular polarized plasma.

Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation.

PubMed

Fácio, Cássio L; Previato, Lígia F; Machado-Paula, Ligiane A; Matheus, Paulo Cs; Araújo, Edilberto

2016-12-01

This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals. Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sperm washing followed by swim-up, and 41 (group 2) by discontinuous density gradient centrifugation. This prospective non-randomized study assessed seminal parameters before and after semen processing. A dependent t-test was used for the same technique to analyze seminal parameters before and after semen processing; an independent t-test was used to compare the results before and after processing for both techniques. The two techniques produced decreases in sample concentration (sperm washing followed by swim-up: P<0.000006; discontinuous density gradient centrifugation: P=0.008457) and increases in motility and normal morphology sperm rates after processing. The difference in sperm motility between the two techniques was not statistically significant. Sperm washing followed by swim-up had better morphology recovery rates than discontinuous density gradient centrifugation (P=0.0095); and the density gradient group had better concentration recovery rates than the swim-up group (P=0.0027). The two methods successfully recovered the minimum sperm values needed to perform intrauterine insemination. Sperm washing followed by swim-up is indicated for semen with high sperm concentration and better morphology recovery rates. Discontinuous density gradient centrifugation produced improved concentration recovery rates.

Density Gradient Columns for Chemical Displays.

ERIC Educational Resources Information Center

Guenther, William B.

1986-01-01

Procedures for preparing density gradient columns for chemical displays are presented. They include displays illustrating acid-base reactions, metal ion equilibria, and liquid density. The lifetime of these metastable displays is surprising, some lasting for months in display cabinets. (JN)

Effects of low central fuelling on density and ion temperature profiles in reversed shear plasmas on JT-60U

NASA Astrophysics Data System (ADS)

Takenaga, H.; Ide, S.; Sakamoto, Y.; Fujita, T.; JT-60 Team

2008-07-01

Effects of low central fuelling on density and ion temperature profiles have been investigated using negative ion based neutral beam injection and electron cyclotron heating (ECH) in reversed shear plasmas on JT-60U. Strong internal transport barrier (ITB) was maintained in density and ion temperature profiles, when central fuelling was decreased by switching positive ion based neutral beam injection to ECH after the strong ITB formation. Similar density and ion temperature ITBs were formed for the low and high central fuelling cases during the plasma current ramp-up phase. Strong correlation between the density gradient and the ion temperature gradient was observed, indicating that particle transport and ion thermal transport are strongly coupled or the density gradient assists the ion temperature ITB formation through suppression of drift wave instabilities such as ion temperature gradient mode. These results support that the density and ion temperature ITBs can be formed under reactor relevant conditions.

Effect of density gradient centrifugation on reactive oxygen species in human semen.

PubMed

Takeshima, Teppei; Yumura, Yasushi; Kuroda, Shinnosuke; Kawahara, Takashi; Uemura, Hiroji; Iwasaki, Akira

2017-06-01

Density gradient centrifugation can separate motile sperm from immotile sperm and other cells for assisted reproduction, but may also remove antioxidants from seminal plasma, resulting in oxidative stress. Therefore, we investigated reactive oxygen species (ROS) concentrations and distribution in semen before and after density gradient centrifugation. We assessed semen volume, sperm concentration, sperm motility, and ROS levels before and after density gradient centrifugation (300 x g for 20 minutes) in 143 semen samples from 118 patients. The ROS removal rate was evaluated in ROS-positive samples and ROS formation rate in ROS-negative samples. Thirty-eight of 143 untreated samples (26.6%) were ROS-positive; sperm motility was significantly lower in these samples than in ROS-negative samples (p < 0.05). After density gradient centrifugation, only seven of the 38 ROS-positive samples (18.42%) exhibited a ROS-positive lower layer (containing motile sperm) with a ROS removal rate of 81.58%, whereas the upper layer was ROS-positive in 24 samples (63.16%). In the ROS-negative group (n = 105), ROS was detected in 19 samples after centrifugation (18.10%, ROS generation rate), of which 18 were ROS-positive only in the upper layer or interface and the other was ROS-positive in both layers. Density gradient centrifugation can separate motile sperm from immotile sperm as well as remove ROS (including newly generated ROS). This data supports the view that density gradient centrifugation can select motile spermatozoa without enhancing oxidative stress. ROS: reactive oxygen species; SOD: superoxide dismutase; GPx: glutathione peroxidase; DNA: deoxyribonucleic acid; DGC: density gradient centrifugation; IUI: intrauterine insemination; IVF: in vitro fertilization; HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; EDTA: ethylenediaminetetraacetic acid; HTF: HEPES-buffered human tubal fluid; IMSI: intracytoplasmic morphologically selected sperm injection; SMAS: sperm motility analyzing system; CASA: computer-assisted semen analyzer; WHO: World Health Organization.

Analytic Expressions for the Gravity Gradient Tensor of 3D Prisms with Depth-Dependent Density

NASA Astrophysics Data System (ADS)

Jiang, Li; Liu, Jie; Zhang, Jianzhong; Feng, Zhibing

2017-12-01

Variable-density sources have been paid more attention in gravity modeling. We conduct the computation of gravity gradient tensor of given mass sources with variable density in this paper. 3D rectangular prisms, as simple building blocks, can be used to approximate well 3D irregular-shaped sources. A polynomial function of depth can represent flexibly the complicated density variations in each prism. Hence, we derive the analytic expressions in closed form for computing all components of the gravity gradient tensor due to a 3D right rectangular prism with an arbitrary-order polynomial density function of depth. The singularity of the expressions is analyzed. The singular points distribute at the corners of the prism or on some of the lines through the edges of the prism in the lower semi-space containing the prism. The expressions are validated, and their numerical stability is also evaluated through numerical tests. The numerical examples with variable-density prism and basin models show that the expressions within their range of numerical stability are superior in computational accuracy and efficiency to the common solution that sums up the effects of a collection of uniform subprisms, and provide an effective method for computing gravity gradient tensor of 3D irregular-shaped sources with complicated density variation. In addition, the tensor computed with variable density is different in magnitude from that with constant density. It demonstrates the importance of the gravity gradient tensor modeling with variable density.

Investigating Students' Ideas About Buoyancy and the Influence of Haptic Feedback

NASA Astrophysics Data System (ADS)

Minogue, James; Borland, David

2016-04-01

While haptics (simulated touch) represents a potential breakthrough technology for science teaching and learning, there is relatively little research into its differential impact in the context of teaching and learning. This paper describes the testing of a haptically enhanced simulation (HES) for learning about buoyancy. Despite a lifetime of everyday experiences, a scientifically sound explanation of buoyancy remains difficult to construct for many. It requires the integration of domain-specific knowledge regarding density, fluid, force, gravity, mass, weight, and buoyancy. Prior studies suggest that novices often focus on only one dimension of the sinking and floating phenomenon. Our HES was designed to promote the integration of the subconcepts of density and buoyant forces and stresses the relationship between the object itself and the surrounding fluid. The study employed a randomized pretest-posttest control group research design and a suite of measures including an open-ended prompt and objective content questions to provide insights into the influence of haptic feedback on undergraduate students' thinking about buoyancy. A convenience sample (n = 40) was drawn from a university's population of undergraduate elementary education majors. Two groups were formed from haptic feedback (n = 22) and no haptic feedback (n = 18). Through content analysis, discernible differences were seen in the posttest explanations sinking and floating across treatment groups. Learners that experienced the haptic feedback made more frequent use of "haptically grounded" terms (e.g., mass, gravity, buoyant force, pushing), leading us to begin to build a local theory of language-mediated haptic cognition.

Stochastic transport models for mixing in variable-density turbulence

NASA Astrophysics Data System (ADS)

Bakosi, J.; Ristorcelli, J. R.

2011-11-01

In variable-density (VD) turbulent mixing, where very-different- density materials coexist, the density fluctuations can be an order of magnitude larger than their mean. Density fluctuations are non-negligible in the inertia terms of the Navier-Stokes equation which has both quadratic and cubic nonlinearities. Very different mixing rates of different materials give rise to large differential accelerations and some fundamentally new physics that is not seen in constant-density turbulence. In VD flows material mixing is active in a sense far stronger than that applied in the Boussinesq approximation of buoyantly-driven flows: the mass fraction fluctuations are coupled to each other and to the fluid momentum. Statistical modeling of VD mixing requires accounting for basic constraints that are not important in the small-density-fluctuation passive-scalar-mixing approximation: the unit-sum of mass fractions, bounded sample space, and the highly skewed nature of the probability densities become essential. We derive a transport equation for the joint probability of mass fractions, equivalent to a system of stochastic differential equations, that is consistent with VD mixing in multi-component turbulence and consistently reduces to passive scalar mixing in constant-density flows.

Development and validation of a critical gradient energetic particle driven Alfven eigenmode transport model for DIII-D tilted neutral beam experiments

DOE PAGES

Waltz, Ronald E.; Bass, Eric M.; Heidbrink, William W.; ...

2015-10-30

Recent experiments with the DIII-D tilted neutral beam injection (NBI) varying the beam energetic particle (EP) source profiles have provided strong evidence that unstable Alfven eigenmodes (AE) drive stiff EP transport at a critical EP density gradient. Here the critical gradient is identified by the local AE growth rate being equal to the local ITG/TEM growth rate at the same low toroidal mode number. The growth rates are taken from the gyrokinetic code GYRO. Simulation show that the slowing down beam-like EP distribution has a slightly lower critical gradient than the Maxwellian. The ALPHA EP density transport code, used tomore » validate the model, combines the low-n stiff EP critical density gradient AE mid-core transport with the energy independent high-n ITG/TEM density transport model controling the central core EP density profile. For the on-axis NBI heated DIII-D shot 146102, while the net loss to the edge is small, about half the birth fast ions are transported from the central core r/a < 0.5 and the central density is about half the slowing down density. Lastly, these results are in good agreement with experimental fast ion pressure profiles inferred from MSE constrained EFIT equilibria.« less

Thermo-chemical evolution of a one-plate planet: application to Mars

NASA Astrophysics Data System (ADS)

Plesa, A.-C.; Breuer, D.

2012-04-01

Little attention has been devoted so far to find a modelling framework able to explain the geophysical implications of the Martian meteorites, the so-called SNC meteorites. Geochemical analysis of the SNC meteorites implies the rapid formation, i.e. before ~4.5 Ga, of three to four isotopically distinct reservoirs that did not remix since then [3]. In [4] the authors argue that a fast overturn of an early fractionated magma ocean may have given origin to a stably stratified mantle with a large density gradient capable to keep the mantle heterogeneous and to prevent mixing due to thermal convection. This model, albeit capable to provide a plausible explanation to the SNC meteorites, suggests a conductive mantle after the overturn which is clearly at odds with the volcanic history of Mars. This is best explained by assuming a convective mantle and partial melting as the principal agents responsible for the generation and evolution of Martian volcanism. In this work, we present an alternative scenario assuming a homogeneous mantle and accounting for compositional changes and melting temperature variations due to mantle depletion, dehydration stiffening of the mantle material due to water partitioning from the minerals into the melt, redistribution of radioactive heat sources between mantle and crust and thermal conductivity decrease in crustal regions. We use the 2D cylindrical - 3D spherical convection code Gaia [1, 2] and to model the above mentioned effects of partial melting we use a Lagrangian, particle based method. Simulation results show that chemical reservoirs, which can be formed due to partial melting when accounting for compositional changes and dehydration stiffening, remain stable over the entire thermal evolution of Mars. However, an initially depleted (i.e. buoyant harzburgite) layer of about 200 km is needed. This depleted layer in an otherwise homogeneous mantle may be the consequence of equilibrium fractionation of a freezing magma ocean where only the residual melt rises to the surface. If the heat released by accretion never allowed for a magma ocean to build, a large amount of partial melting of about 20% in the earliest stage is required to form such a buoyant layer. These models show an active convective interior and long lived partial melt production, which agrees with the volcanic history of Mars [5].

X-ray tomography characterization of density gradient aerogel in laser targets

NASA Astrophysics Data System (ADS)

Borisenko, L.; Orekhov, A.; Musgrave, C.; Nazarov, W.; Merkuliev, Yu; Borisenko, N.

2016-04-01

The low-density solid laser target characterization studies begun with the SkyScan 1074 computer microtomograph (CMT) [1, 2] are now continued with higher resolution of SkyScan 1174. The research is particularly focused on the possibility to obtain, control and measure precisely the gradient density polymers for laser target production. Repeatability of the samples and possibility to obtain stable gradients are analysed. The measurements were performed on the mm-scale divinyl benzene (DVB) rods.

Interconfigurational energies in transition-metal atoms using gradient-corrected density-functional theory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kutzler, F.W.; Painter, G.S.

1991-03-15

The rapid variation of charge and spin densities in atoms and molecules provides a severe test for local-density-functional theory and for the use of gradient corrections. In the study reported in this paper, we use the Langreth, Mehl, and Hu (LMH) functional and the generalized gradient approximation (GGA) of Perdew and Yue to calculate {ital s}-{ital d} transition energies, 4{ital s} ionization energies, and 3{ital d} ionization energies for the 3{ital d} transition-metal atoms. These calculations are compared with results from the local-density functional of Vosko, Wilk, and Nusair. By comparison with experimental energies, we find that the gradient functionalsmore » are only marginally more successful than the local-density approximation in calculating energy differences between states in transition-metal atoms. The GGA approximation is somewhat better than the LMH functional for most of the atoms studied, although there are several exceptions.« less

Semi-local machine-learned kinetic energy density functional with third-order gradients of electron density

NASA Astrophysics Data System (ADS)

Seino, Junji; Kageyama, Ryo; Fujinami, Mikito; Ikabata, Yasuhiro; Nakai, Hiromi

2018-06-01

A semi-local kinetic energy density functional (KEDF) was constructed based on machine learning (ML). The present scheme adopts electron densities and their gradients up to third-order as the explanatory variables for ML and the Kohn-Sham (KS) kinetic energy density as the response variable in atoms and molecules. Numerical assessments of the present scheme were performed in atomic and molecular systems, including first- and second-period elements. The results of 37 conventional KEDFs with explicit formulae were also compared with those of the ML KEDF with an implicit formula. The inclusion of the higher order gradients reduces the deviation of the total kinetic energies from the KS calculations in a stepwise manner. Furthermore, our scheme with the third-order gradient resulted in the closest kinetic energies to the KS calculations out of the presented functionals.

46 CFR 160.047-4 - Construction.

Code of Federal Regulations, 2012 CFR

2012-10-01

... AF-1, CFM-1, and CFS-1. The buoyant pad inserts for Models AF-1, CFM-1, and CFS-1 buoyant vests shall...)—Distribution of Fibrous Glass in Buoyant Pad Inserts Model AF-1 (minimum) Model CFM-1 (minimum) Model CFS-1... and CFM-1 Each Models CKS-1 and CFS-1 Each Front pads 61/4 pounds ±1/4 pound 41/4 pounds ±1/4 pound 23...

46 CFR 160.047-4 - Construction.

Code of Federal Regulations, 2011 CFR

2011-10-01

... AF-1, CFM-1, and CFS-1. The buoyant pad inserts for Models AF-1, CFM-1, and CFS-1 buoyant vests shall...)—Distribution of Fibrous Glass in Buoyant Pad Inserts Model AF-1 (minimum) Model CFM-1 (minimum) Model CFS-1... and CFM-1 Each Models CKS-1 and CFS-1 Each Front pads 61/4 pounds ±1/4 pound 41/4 pounds ±1/4 pound 23...

Properties of ion temperature gradient and trapped electron modes in tokamak plasmas with inverted density profiles

NASA Astrophysics Data System (ADS)

Du, Huarong; Jhang, Hogun; Hahm, T. S.; Dong, J. Q.; Wang, Z. X.

2017-12-01

We perform a numerical study of linear stability of the ion temperature gradient (ITG) mode and the trapped electron mode (TEM) in tokamak plasmas with inverted density profiles. A local gyrokinetic integral equation is applied for this study. From comprehensive parametric scans, we obtain stability diagrams for ITG modes and TEMs in terms of density and temperature gradient scale lengths. The results show that, for the inverted density profile, there exists a normalized threshold temperature gradient above which the ITG mode and the TEM are either separately or simultaneously unstable. The instability threshold of the TEM for the inverted density profile is substantially different from that for normal and flat density profiles. In addition, deviations are found on the ITG threshold from an early analytic theory in sheared slab geometry with the adiabatic electron response [T. S. Hahm and W. M. Tang, Phys. Fluids B 1, 1185 (1989)]. A possible implication of this work on particle transport in pellet fueled tokamak plasmas is discussed.

Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures.

PubMed

Shen, Chen; Julius, Ethan F; Tyree, Timothy J; Dan, Ritwik; Moreau, David W; Thorne, Robert

2017-06-28

We demonstrate a method for determining the vitreous phase cryogenic temperature densities of aqueous mixtures, and other samples that require rapid cooling, to prepare the desired cryogenic temperature phase. Microliter to picoliter size drops are cooled by projection into a liquid nitrogen-argon (N2-Ar) mixture. The cryogenic temperature phase of the drop is evaluated using a visual assay that correlates with X-ray diffraction measurements. The density of the liquid N2-Ar mixture is adjusted by adding N2 or Ar until the drop becomes neutrally buoyant. The density of this mixture and thus of the drop is determined using a test mass and Archimedes principle. With appropriate care in drop preparation, management of gas above the liquid cryogen mixture to minimize icing, and regular mixing of the cryogenic mixture to prevent density stratification and phase separation, densities accurate to <0.5% of drops as small as 50 pL can readily be determined. Measurements on aqueous cryoprotectant mixtures provide insight into cryoprotectant action, and provide quantitative data to facilitate thermal contraction matching in biological cryopreservation.

Density gradients at hydrogel interfaces for enhanced cell penetration.

PubMed

Simona, B R; Hirt, L; Demkó, L; Zambelli, T; Vörös, J; Ehrbar, M; Milleret, V

2015-04-01

We report that stiffness gradients facilitate infiltration of cells through otherwise cell-impermeable hydrogel interfaces. By enabling the separation of hydrogel manufacturing and cell seeding, and by improving cell colonization of additively manufactured hydrogel elements, interfacial density gradients present a promising strategy to progress in the creation of 3D tissue models.

Merging Features and Optical-Near Infrared Color Gradients of Early-type Galaxies

NASA Astrophysics Data System (ADS)

Kim, Duho; Im, M.

2012-01-01

It has been suggested that merging plays an important role in the formation and the evolution of early-type galaxies (ETGs). Optical-NIR color gradients of ETGs in high density environments are found to be less steep than those of ETGs in low density environments, hinting frequent merger activities in ETGs in high density environments. In order to examine if the flat color gradients are the result of dry mergers, we studied the relations between merging features, color gradient, and environments of 198 low redshift ETGs selected from Sloan Digital Sky Survey (SDSS) Stripe82. Near Infrared (NIR) images are taken from UKIRT Infrared Deep Sky Survey (UKIDSS) Large Area Survey (LAS). Color(r-K) gradients of ETGs with tidal features are a little flatter than relaxed ETGs, but not significant. We found that massive (>1011.3 M⊙) relaxed ETGs have 2.5 times less scattered color gradients than less massive ETGs. The less scattered color gradients of massive ETGs could be evidence of dry merger processes in the evolution of massive ETGs. We found no relation between color gradients of ETGs and their environments.

Method and means for a spatial and temporal probe for laser-generated plumes based on density gradients

DOEpatents

Yeung, Edward S.; Chen, Guoying

1990-05-01

A method and means for a spatial and temporal probe for laser generated plumes based on density gradients includes generation of a plume of vaporized material from a surface by an energy source. The probe laser beam is positioned so that the plume passes through the probe laser beam. Movement of the probe laser beam caused by refraction from the density gradient of the plume is monitored. Spatial and temporal information, correlated to one another, is then derived.

Identification of a calmodulin-regulated Ca2+-ATPase in the endoplasmic reticulum

NASA Technical Reports Server (NTRS)

Hong, B.; Ichida, A.; Wang, Y.; Gens, J. S.; Pickard, B. G.; Harper, J. F.; Evans, M. L. (Principal Investigator)

1999-01-01

A unique subfamily of calmodulin-dependent Ca2+-ATPases was recently identified in plants. In contrast to the most closely related pumps in animals, plasma membrane-type Ca2+-ATPases, members of this new subfamily are distinguished by a calmodulin-regulated autoinhibitor located at the N-terminal instead of a C-terminal end. In addition, at least some isoforms appear to reside in non-plasma membrane locations. To begin delineating their functions, we investigated the subcellular localization of isoform ACA2p (Arabidopsis Ca2+-ATPase, isoform 2 protein) in Arabidopsis. Here we provide evidence that ACA2p resides in the endoplasmic reticulum (ER). In buoyant density sucrose gradients performed with and without Mg2+, ACA2p cofractionated with an ER membrane marker and a typical "ER-type" Ca2+-ATPase, ACA3p/ECA1p. To visualize its subcellular localization, ACA2p was tagged with a green fluorescence protein at its C terminus (ACA2-GFPp) and expressed in transgenic Arabidopsis. We collected fluorescence images from live root cells using confocal and computational optical-sectioning microscopy. ACA2-GFPp appeared as a fluorescent reticulum, consistent with an ER location. In addition, we observed strong fluorescence around the nuclei of mature epidermal cells, which is consistent with the hypothesis that ACA2p may also function in the nuclear envelope. An ER location makes ACA2p distinct from all other calmodulin-regulated pumps identified in plants or animals.

Apical sorting of a voltage- and Ca2+-activated K+ channel α-subunit in Madin-Darby canine kidney cells is independent of N-glycosylation

PubMed Central

Bravo-Zehnder, Marcela; Orio, Patricio; Norambuena, Andrés; Wallner, Martin; Meera, Pratap; Toro, Ligia; Latorre, Ramón; González, Alfonso

2000-01-01

The voltage- and Ca2+-activated K+ (KV,Ca) channel is expressed in a variety of polarized epithelial cells seemingly displaying a tissue-dependent apical-to-basolateral regionalization, as revealed by electrophysiology. Using domain-specific biotinylation and immunofluorescence we show that the human channel KV,Ca α-subunit (human Slowpoke channel, hSlo) is predominantly found in the apical plasma membrane domain of permanently transfected Madin-Darby canine kidney cells. Both the wild-type and a mutant hSlo protein lacking its only potential N-glycosylation site were efficiently transported to the cell surface and concentrated in the apical domain even when they were overexpressed to levels 200- to 300-fold higher than the density of intrinsic Slo channels. Furthermore, tunicamycin treatment did not prevent apical segregation of hSlo, indicating that endogenous glycosylated proteins (e.g., KV,Ca β-subunits) were not required. hSlo seems to display properties for lipid-raft targeting, as judged by its buoyant distribution in sucrose gradients after extraction with either detergent or sodium carbonate. The evidence indicates that the hSlo protein possesses intrinsic information for transport to the apical cell surface through a mechanism that may involve association with lipid rafts and that is independent of glycosylation of the channel itself or an associated protein. Thus, this particular polytopic model protein shows that glycosylation-independent apical pathways exist for endogenous membrane proteins in Madin-Darby canine kidney cells. PMID:11069304

Isolation and characterization of lipid rafts in Emiliania huxleyi: a role for membrane microdomains in host-virus interactions.

PubMed

Rose, Suzanne L; Fulton, James M; Brown, Christopher M; Natale, Frank; Van Mooy, Benjamin A S; Bidle, Kay D

2014-04-01

Coccolithoviruses employ a suite of glycosphingolipids (GSLs) to successfully infect the globally important coccolithophore Emiliania huxleyi. Lipid rafts, chemically distinct membrane lipid microdomains that are enriched in GSLs and are involved in sensing extracellular stimuli and activating signalling cascades through protein-protein interactions, likely play a fundamental role in host-virus interactions. Using combined lipidomics, proteomics and bioinformatics, we isolated and characterized the lipid and protein content of lipid rafts from control E. huxleyi cells and those infected with EhV86, the type strain for Coccolithovirus. Lipid raft-enriched fractions were isolated and purified as buoyant, detergent-resistant membranes (DRMs) in OptiPrep density gradients. Transmission electron microscopy of vesicle morphology, polymerase chain reaction amplification of the EhV major capsid protein gene and immunoreactivity to flotillin antisera served as respective physical, molecular and biochemical markers. Subsequent lipid characterization of DRMs via high performance liquid chromatography-triple quadrapole mass spectrometry revealed four distinct GSL classes. Parallel proteomic analysis confirmed flotillin as a major lipid raft protein, along with a variety of proteins affiliated with host defence, programmed cell death and innate immunity pathways. The detection of an EhV86-encoded C-type lectin-containing protein confirmed that infection occurs at the interface between lipid rafts and cellular stress/death pathways via specific GSLs and raft-associated proteins. © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd.

How well can an amoeba climb?

PubMed Central

Fukui, Yoshio; Uyeda, Taro Q. P.; Kitayama, Chikako; Inoué, Shinya

2000-01-01

We report here our efforts to measure the crawling force generated by cells undergoing amoeboid locomotion. In a centrifuge microscope, acceleration was increased until amoebae of Dictyostelium discoideum were “stalled” or no longer able to “climb up.” The “apparent weight” of the amoebae at stalling rpm in myosin mutants depended on the presence of myosin II (but not myosins IA and IB) and paralleled the cortical strength of the cells. Surprisingly, however, the cell stalled not only in low-density media as expected but also in media with densities greater than the cell density where the buoyant force should push the amoeba upward. We find that the leading pseudopod is bent under centrifugal force in all stalled amoebae, suggesting that this pseudopod is very dense indeed. This finding also suggests that directional cell locomotion against resistive forces requires a turgid forward-pointing pseudopod, most likely sustained by cortical actomyosin II. PMID:10963666

Buoyant miscible displacement flow of shear-thinning fluids: Experiments and Simulations

NASA Astrophysics Data System (ADS)

Ale Etrati Khosroshahi, Seyed Ali; Frigaard, Ian

2017-11-01

We study displacement flow of two miscible fluids with density and viscosity contrast in an inclined pipe. Our focus is mainly on displacements where transverse mixing is not significant and thus a two-layer, stratified flow develops. Our experiments are carried out in a long pipe, covering a wide range of flow-rates, inclination angles and viscosity ratios. Density and viscosity contrasts are achieved by adding Glycerol and Xanthan gum to water, respectively. At each angle, flow rate and viscosity ratio are varied and density contrast is fixed. We identify and map different flow regimes, instabilities and front dynamics based on Fr , Re / Frcosβ and viscosity ratio m. The problem is also studied numerically to get a better insight into the flow structure and shear-thinning effects. Numerical simulations are completed using OpenFOAM in both pipe and channel geometries and are compared against the experiments. Schlumberger, NSERC.

Investigating sea level rise due to global warming in the teaching laboratory using Archimedes’ principle

NASA Astrophysics Data System (ADS)

Hughes, Stephen; Pearce, Darren

2015-11-01

A teaching laboratory experiment is described that uses Archimedes’ principle to precisely investigate the effect of global warming on the oceans. A large component of sea level rise is due to the increase in the volume of water due to the decrease in water density with increasing temperature. Water close to 0 °C is placed in a beaker and a glass marble hung from an electronic balance immersed in the water. As the water warms, the weight of the marble increases as the water is less buoyant due to the decrease in density. In the experiment performed in this paper a balance with a precision of 0.1 mg was used with a marble 40.0 cm3 and mass of 99.3 g, yielding water density measurements with an average error of -0.008 ± 0.011%.

Product suitability of wood...determined by density gradients across growth rings

Treesearch

Robert M. Echols

1972-01-01

The suitability of wood for various uses can be determined by synthesizing single growth-ring density curves from accumulated means of wood density classes. Wood density gradients across growth rings were measured in large increment cores from 46-year-old ponderosa pines (Pinus ponderosa Laws.) by using X-rays. Of the 48 trees analyzed, 36 had been...

Numerical Study on Density Gradient Carbon-Carbon Composite for Vertical Launching System

NASA Astrophysics Data System (ADS)

Yoon, Jin-Young; Kim, Chun-Gon; Lim, Juhwan

2018-04-01

This study presents new carbon-carbon (C/C) composite that has a density gradient within single material, and estimates its heat conduction performance by a numerical method. To address the high heat conduction of a high-density C/C, which can cause adhesion separation in the steel structures of vertical launching systems, density gradient carbon-carbon (DGCC) composite is proposed due to its exhibiting low thermal conductivity as well as excellent ablative resistance. DGCC is manufactured by hybridizing two different carbonization processes into a single carbon preform. One part exhibits a low density using phenolic resin carbonization to reduce heat conduction, and the other exhibits a high density using thermal gradient-chemical vapor infiltration for excellent ablative resistance. Numerical analysis for DGCC is performed with a heat conduction problem, and internal temperature distributions are estimated by the forward finite difference method. Material properties of the transition density layer, which is inevitably formed during DGCC manufacturing, are assumed to a combination of two density layers for numerical analysis. By comparing numerical results with experimental data, we validate that DGCC exhibits a low thermal conductivity, and it can serve as highly effective ablative material for vertical launching systems.

A refined model of sedimentary rock cover in the southeastern part of the Congo basin from GOCE gravity and vertical gravity gradient observations

NASA Astrophysics Data System (ADS)

Martinec, Zdeněk; Fullea, Javier

2015-03-01

We aim to interpret the vertical gravity and vertical gravity gradient of the GOCE-GRACE combined gravity model over the southeastern part of the Congo basin to refine the published model of sedimentary rock cover. We use the GOCO03S gravity model and evaluate its spherical harmonic representation at or near the Earth's surface. In this case, the gradiometry signals are enhanced as compared to the original measured GOCE gradients at satellite height and better emphasize the spatial pattern of sedimentary geology. To avoid aliasing, the omission error of the modelled gravity induced by the sedimentary rocks is adjusted to that of the GOCO03S gravity model. The mass-density Green's functions derived for the a priori structure of the sediments show a slightly greater sensitivity to the GOCO03S vertical gravity gradient than to the vertical gravity. Hence, the refinement of the sedimentary model is carried out for the vertical gravity gradient over the basin, such that a few anomalous values of the GOCO03S-derived vertical gravity gradient are adjusted by refining the model. We apply the 5-parameter Helmert's transformation, defined by 2 translations, 1 rotation and 2 scale parameters that are searched for by the steepest descent method. The refined sedimentary model is only slightly changed with respect to the original map, but it significantly improves the fit of the vertical gravity and vertical gravity gradient over the basin. However, there are still spatial features in the gravity and gradiometric data that remain unfitted by the refined model. These may be due to lateral density variation that is not contained in the model, a density contrast at the Moho discontinuity, lithospheric density stratifications or mantle convection. In a second step, the refined sedimentary model is used to find the vertical density stratification of sedimentary rocks. Although the gravity data can be interpreted by a constant sedimentary density, such a model does not correspond to the gravitational compaction of sedimentary rocks. Therefore, the density model is extended by including a linear increase in density with depth. Subsequent L2 and L∞ norm minimization procedures are applied to find the density parameters by adjusting both the vertical gravity and the vertical gravity gradient. We found that including the vertical gravity gradient in the interpretation of the GOCO03S-derived data reduces the non-uniqueness of the inverse gradiometric problem for density determination. The density structure of the sedimentary formations that provide the optimum predictions of the GOCO03S-derived gravity and vertical gradient of gravity consists of a surface density contrast with respect to surrounding rocks of 0.24-0.28 g/cm3 and its decrease with depth of 0.05-0.25 g/cm3 per 10 km. Moreover, the case where the sedimentary rocks are gravitationally completely compacted in the deepest parts of the basin is supported by L∞ norm minimization. However, this minimization also allows a remaining density contrast at the deepest parts of the sedimentary basin of about 0.1 g/cm3.

Density functional theory for molecular and periodic systems using density fitting and continuous fast multipole method: Analytical gradients.

PubMed

Łazarski, Roman; Burow, Asbjörn Manfred; Grajciar, Lukáš; Sierka, Marek

2016-10-30

A full implementation of analytical energy gradients for molecular and periodic systems is reported in the TURBOMOLE program package within the framework of Kohn-Sham density functional theory using Gaussian-type orbitals as basis functions. Its key component is a combination of density fitting (DF) approximation and continuous fast multipole method (CFMM) that allows for an efficient calculation of the Coulomb energy gradient. For exchange-correlation part the hierarchical numerical integration scheme (Burow and Sierka, Journal of Chemical Theory and Computation 2011, 7, 3097) is extended to energy gradients. Computational efficiency and asymptotic O(N) scaling behavior of the implementation is demonstrated for various molecular and periodic model systems, with the largest unit cell of hematite containing 640 atoms and 19,072 basis functions. The overall computational effort of energy gradient is comparable to that of the Kohn-Sham matrix formation. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R.; Torczynski, John R.; Brady, Patrick V.; Gallis, Michail; Brooks, Carlton F.

2014-06-17

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R; Torczynski, John R; Brady, Patrick V; Gallis, Michail; Brooks, Carlton F

2013-09-17

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R; Torczynski, John R; Brady, Patrick V; Gallis, Michail; Brooks, Carlton F

2013-11-19

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Tripolar vortex formation in dense quantum plasma with ion-temperature-gradients

NASA Astrophysics Data System (ADS)

Qamar, Anisa; Ata-ur-Rahman, Mirza, Arshad M.

2012-05-01

We have derived system of nonlinear equations governing the dynamics of low-frequency electrostatic toroidal ion-temperature-gradient mode for dense quantum magnetoplasma. For some specific profiles of the equilibrium density, temperature, and ion velocity gradients, the nonlinear equations admit a stationary solution in the form of a tripolar vortex. These results are relevant to understand nonlinear structure formation in dense quantum plasmas in the presence of equilibrium ion-temperature and density gradients.

Effects of finite-size particles on the turbulent flows in a square duct

NASA Astrophysics Data System (ADS)

Yu, Zhaosheng; Lin, Zhaowu; Shao, Xueming; Wang, Lian-Ping

2015-11-01

Fully resolved numerical simulations of the particle-laden turbulent flows in a square duct are performed with a direct-forcing fictitious domain method. The effects of the finite-size particles on the mean and root-mean-square (RMS) velocities are investigated at the friction Reynolds number of 150 (based on the friction velocity and half duct width) and the particle volume fractions ranging from 0.78% to 7.07%. For the neutrally buoyant case, our results show that the mean secondary flow is enhanced and its circulation center shifts closer to the center of the duct cross-section when the particles are added. The reason for the particle effect on the mean secondary flow is analyzed by examining the terms in the mean streamwise vorticity equation. The particles enhance the wall-tangential component of the RMS velocity (i.e. Reynolds normal stress) more than its wall-normal component in the near-wall region near the corners, resulting in the enhancement in the gradients of the normal stress difference, which we think is mainly responsible for the enhancement in the mean secondary flow. The particles accumulate preferentially in the near-corner region in the neutrally buoyant case. In addition, the effects of particle sedimentation are examined at different Shields numbers. The work was supported by the National Natural Science Foundation of China (11372275) and Research Fund for the Doctoral Program of Higher Education of China (20130101110035).

Sensor Fish: an autonomous sensor package for characterizing complex flow fields and fish passage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Deng, Zhiqun; Martinez, Jayson J.; Lu, Jun

Fish passing through dams or other hydraulic structures may be injured or killed despite advances in turbine design, project operations, and other fish bypass systems. The Sensor Fish (SF) device is an autonomous sensor package that characterizes the physical conditions and stressors to which fish are exposed during passage through hydro facilities. It was designed to move passively as a neutrally buoyant object through severe hydraulic environments, while collecting high-resolution sensor data. Since its first generation1, the SF device has been successfully deployed in many fish passage studies and has evolved to be a major tool for characterizing fish passagemore » conditions during fish passage in the Columbia River Basin. To better accelerate hydropower development, the U.S. Department of Energy Water Power Program provided funding to develop a new generation (Gen 2 SF) to incorporate more capabilities and accommodate a wider range of users over a broader range of turbine designs and operating environments. The Gen 2 SF (Figure 1) is approximately the size and density of a yearling salmon smolt and is nearly neutrally buoyant. It contains three-dimensional (3D) rotation sensors, 3D linear acceleration sensors, a pressure sensor, a temperature sensor, a 3D orientation sensor, a radiofrequency (RF) transmitter, and a recovery module2. A low-power microcontroller collects data from the sensors and stores up to 5 min of data on internal flash memory at a sampling frequency of 2048 Hz. The recovery module makes the SF positively buoyant after a pre-programmed period of time, causing it to float to the surface for recovery.« less

Generalization of the van der Pauw Method: Analyzing Longitudinal Magnetoresistance Asymmetry to Quantify Doping Gradients

NASA Astrophysics Data System (ADS)

Grayson, M.; Zhou, Wang; Yoo, Heun-Mo; Prabhu-Gaunkar, S.; Tiemann, L.; Reichl, C.; Wegscheider, W.

A longitudinal magnetoresistance asymmetry (LMA) between a positive and negative magnetic field is known to occur in both the extreme quantum limit and the classical Drude limit in samples with a nonuniform doping density. By analyzing the current stream function in van der Pauw measurement geometry, it is shown that the electron density gradient can be quantitatively deduced from this LMA in the Drude regime. Results agree with gradients interpolated from local densities calibrated across an entire wafer, establishing a generalization of the van der Pauw method to quantify density gradients. Results will be shown of various semoconductor systems where this method is applied, from bulk doped semiconductors, to exfoliated 2D materials. McCormick Catalyst Award from Northwestern University, EECS Bridge Funding, and AFOSR FA9550-15-1-0247.

Temperature Gradients on the Cell Wall in the Critical Viscosity Experiment

NASA Technical Reports Server (NTRS)

Berg, Robert F.; Moldover, Michael R.

1993-01-01

Because of the diverging susceptibility delta rho/delta Tau near the liquid-vapor critical point, temperature gradients must be kept small to maintain adequate sample homogeneity. In our Science Requirements Document we paid particular attention to radial density gradients caused by equilibration of the xenon sample. Axial density gradients were addressed through the requirement that the cell's copper wall have a gradient less than 22 microK/m. This report re-examines the cell wall's temperature distribution in more detail by estimating all known significant contributions to temperature differences on the cell's wall.

46 CFR 160.049-4 - Construction and workmanship.

Code of Federal Regulations, 2011 CFR

2011-10-01

... MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Cushion Plastic Foam... box type filled with unicellular plastic foam buoyant material. Such cushions consist essentially of a...

46 CFR 160.049-4 - Construction and workmanship.

Code of Federal Regulations, 2014 CFR

2014-10-01

... MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Cushion Plastic Foam... box type filled with unicellular plastic foam buoyant material. Such cushions consist essentially of a...

Binding in pair potentials of liquid simple metals from nonlocality in electronic kinetic energy

NASA Technical Reports Server (NTRS)

Perrot, F.; March, N. H.

1990-01-01

The paper presents an explicit expression for the pair potential in liquid simple metals from low-order density-gradient theory when the superposition of single-center displaced charges is employed. Numerical results are presented for the gradient expansion pair interaction in liquid Na and Be. The low-order density-gradient equation for the pair potential is presented.

MEMS cantilever based magnetic field gradient sensor

NASA Astrophysics Data System (ADS)

Dabsch, Alexander; Rosenberg, Christoph; Stifter, Michael; Keplinger, Franz

2017-05-01

This paper describes major contributions to a MEMS magnetic field gradient sensor. An H-shaped structure supported by four arms with two circuit paths on the surface is designed for measuring two components of the magnetic flux density and one component of the gradient. The structure is produced from silicon wafers by a dry etching process. The gold leads on the surface carry the alternating current which interacts with the magnetic field component perpendicular to the direction of the current. If the excitation frequency is near to a mechanical resonance, vibrations with an amplitude within the range of 1-103 nm are expected. Both theoretical (simulations and analytic calculations) and experimental analysis have been carried out to optimize the structures for different strength of the magnetic gradient. In the same way the impact of the coupling structure on the resonance frequency and of different operating modes to simultaneously measure two components of the flux density were tested. For measuring the local gradient of the flux density the structure was operated at the first symmetrical and the first anti-symmetrical mode. Depending on the design, flux densities of approximately 2.5 µT and gradients starting from 1 µT mm-1 can be measured.

Method and means for a spatial and temporal probe for laser-generated plumes based on density gradients

DOEpatents

Yeung, E.S.; Chen, G.

1990-05-01

A method and means are disclosed for a spatial and temporal probe for laser generated plumes based on density gradients includes generation of a plume of vaporized material from a surface by an energy source. The probe laser beam is positioned so that the plume passes through the probe laser beam. Movement of the probe laser beam caused by refraction from the density gradient of the plume is monitored. Spatial and temporal information, correlated to one another, is then derived. 15 figs.

Parametric study of modern airship productivity

NASA Technical Reports Server (NTRS)

Ardema, M. D.; Flaig, K.

1980-01-01

A method for estimating the specific productivity of both hybrid and fully buoyant airships is developed. Various methods of estimating structural weight of deltoid hybrids are discussed and a derived weight estimating relationship is presented. Specific productivity is used as a figure of merit in a parametric study of fully buoyant ellipsoidal and deltoid hybrid semi-buoyant vehicles. The sensitivity of results as a function of assumptions is also determined. No airship configurations were found to have superior specific productivity to transport airplanes.

Stochastic Mapping for Chemical Plume Source Localization With Application to Autonomous Hydrothermal Vent Discovery

DTIC Science & Technology

2007-02-01

rise froom the seafloor a buoyant hydrothermal plume will have expanded laterally fromn oil the order of a few centimeters at an...diameters of rise height [20]. Detections of buoyant plume effluent are likely when the vehicle passes near the plume centerline; however, the in...the vertical extent of the plume . I will refer to this figure, W0 = 0.1 m/s, subsequently as the "canonical rise rate" for buoyant hydrothermal plumes

Evaluation of effects of geometrical parameters on density distribution in compaction of PM gears

NASA Astrophysics Data System (ADS)

Khodaee, Alireza; Melander, Arne

2017-10-01

The usage of powder metallurgy (PM) for manufacturing of transmission components in automotive industries has been studied by many researchers. PM components have become of interest in recent years due to advancements in post processing possibilities such as hot isostatic pressing (HIP). Still in many of the forming process routes for making components from PM materials, the compaction of the powder into green component is the first step. Compaction is required to put the powder into the near net shape of the desired component and it causes a density gradient in the body of the green component. Basically the friction between powder particles and between the powder particles and die walls are the well-known roots for such density gradients in the compacted component. Looking at forming of PM gears, the gradient in density is one of the most important roots of problems in the processing of PM gears as well. That is because making a gear with full density and no pores will be very costly if large density gradients exist in the green component. The purpose of this study is to find the possible relations between the gear geometry and the density gradients in the green component after compaction in addition to the friction effects. For this purpose several gears should be tested. To reduce the research costs, the finite element (FE) method is used. First a FE model of the compaction process is developed and verified. To investigate the relations between the density gradients and the gear parameters such as addendum diameter (da) and the face width (b) several gear geometries have been studied. The compaction of selected gears is simulated using the FE model. The simulations results which are the distribution of density in the green component are evaluated and discussed and conclusion are made based on them.

Correlations between quasi-coherent fluctuations and the pedestal evolution during the inter-edge localized modes phase on DIII-D

DOE PAGES

Diallo, A.; Groebner, R. J.; Rhodes, T. L.; ...

2015-05-15

Direct measurements of the pedestal recovery during an edge-localized mode cycle provide evidence that quasi-coherent fluctuations (QCFs) play a role in the inter-ELM pedestal dynamics. When using fast Thomson scattering measurements, we found that the pedestal density and temperature evolutions are probed on sub-millisecond time scales to show a fast recovery of the density gradient compared to the temperature gradient. The temperature gradient appears to provide a drive for the onset of quasi-coherent fluctuations (as measured with the magnetic probe and the density diagnostics) localized in the pedestal. The amplitude evolution of these QCFs tracks the temperature gradient evolution includingmore » its saturation. Such correlation suggests that these QCFs play a key role in limiting the pedestal temperature gradient. Moreover, the saturation of the QCFs coincides with the pressure gradient reaching the kinetic-ballooning mode (KBM) critical gradient as predicted by EPED1. Furthermore, linear microinstability analysis using GS2 indicates that the steep gradient is near the KBM threshold. Finally, the modeling and the observations together suggest that QCFs are consistent with dominant KBMs, although microtearing cannot be excluded as subdominant.« less

46 CFR 160.049-7 - Procedure for approval.

Code of Federal Regulations, 2011 CFR

2011-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Cushion Plastic Foam § 160.049-7... be granted separately to each manufacturer for each unicellular plastic foam buoyant cushion he...

46 CFR 160.049-7 - Procedure for approval.

Code of Federal Regulations, 2010 CFR

2010-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Cushion Plastic Foam § 160.049-7... be granted separately to each manufacturer for each unicellular plastic foam buoyant cushion he...

46 CFR 160.049-7 - Procedure for approval.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Cushion Plastic Foam § 160.049-7... be granted separately to each manufacturer for each unicellular plastic foam buoyant cushion he...

Floating liquid phase in sedimenting colloid-polymer mixtures.

PubMed

Schmidt, Matthias; Dijkstra, Marjolein; Hansen, Jean-Pierre

2004-08-20

Density functional theory and computer simulation are used to investigate sedimentation equilibria of colloid-polymer mixtures within the Asakura-Oosawa-Vrij model of hard sphere colloids and ideal polymers. When the ratio of buoyant masses of the two species is comparable to the ratio of differences in density of the coexisting bulk (colloid) gas and liquid phases, a stable "floating liquid" phase is found, i.e., a thin layer of liquid sandwiched between upper and lower gas phases. The full phase diagram of the mixture under gravity shows coexistence of this floating liquid phase with a single gas phase or a phase involving liquid-gas equilibrium; the phase coexistence lines meet at a triple point. This scenario remains valid for general asymmetric binary mixtures undergoing bulk phase separation.

Pervasive Eclogitization Due to Brittle Deformation and Rehydration of Subducted Basement: Effects on Continental Recycling?

NASA Astrophysics Data System (ADS)

Engi, Martin; Giuntoli, Francesco; Lanari, Pierre; Burn, Marco; Kunz, Barbara; Bouvier, Anne-Sophie

2018-03-01

The buoyancy of continental crust opposes its subduction to mantle depths, except where mineral reactions substantially increase rock density. Sluggish kinetics limit such densification, especially in dry rocks, unless deformation and hydrous fluids intervene. Here we document how hydrous fluids in the subduction channel invaded lower crustal granulites at 50-60 km depth through a dense network of probably seismically induced fractures. We combine analyses of textures and mineral composition with thermodynamic modeling to reconstruct repeated stages of interaction, with pulses of high-pressure (HP) fluid at 650-670°C, rehydrating the initially dry rocks to micaschists. SIMS oxygen isotopic data of quartz indicate fluids of crustal composition. HP growth rims in allanite and zircon show uniform U-Th-Pb ages of ˜65 Ma and indicate that hydration occurred during subduction, at eclogite facies conditions. Based on this case study in the Sesia Zone (Western Italian Alps), we conclude that continental crust, and in particular deep basement fragments, during subduction can behave as substantial fluid sinks, not sources. Density modeling indicates a bifurcation in continental recycling: Chiefly mafic crust, once it is eclogitized to >60%, are prone to end up in a subduction graveyard, such as is tomographically evident beneath the Alps at ˜550 km depth. By contrast, dominantly felsic HP fragments and mafic granulites remain positively buoyant and tend be incorporated into an orogen and be exhumed with it. Felsic and intermediate lithotypes remain positively buoyant even where deformation and fluid percolation allowed them to equilibrate at HP.

Development and validation of a critical gradient energetic particle driven Alfven eigenmode transport model for DIII-D tilted neutral beam experiments

NASA Astrophysics Data System (ADS)

Waltz, R. E.; Bass, E. M.; Heidbrink, W. W.; VanZeeland, M. A.

2015-11-01

Recent experiments with the DIII-D tilted neutral beam injection (NBI) varying the beam energetic particle (EP) source profiles have provided strong evidence that unstable Alfven eigenmodes (AE) drive stiff EP transport at a critical EP density gradient [Heidbrink et al 2013 Nucl. Fusion 53 093006]. Here the critical gradient is identified by the local AE growth rate being equal to the local ITG/TEM growth rate at the same low toroidal mode number. The growth rates are taken from the gyrokinetic code GYRO. Simulation show that the slowing down beam-like EP distribution has a slightly lower critical gradient than the Maxwellian. The ALPHA EP density transport code [Waltz and Bass 2014 Nucl. Fusion 54 104006], used to validate the model, combines the low-n stiff EP critical density gradient AE mid-core transport with the Angioni et al (2009 Nucl. Fusion 49 055013) energy independent high-n ITG/TEM density transport model controling the central core EP density profile. For the on-axis NBI heated DIII-D shot 146102, while the net loss to the edge is small, about half the birth fast ions are transported from the central core r/a  <  0.5 and the central density is about half the slowing down density. These results are in good agreement with experimental fast ion pressure profiles inferred from MSE constrained EFIT equilibria.

Steep, Transient Density Gradients in the Martian Ionosphere Similar to the Ionopause at Venus

NASA Astrophysics Data System (ADS)

Duru, Firdevs; Gurnett, Donald; Frahm, Rudy; Winningham, D. L.; Morgan, David; Howes, Gregory

Using Mars Advanced Radar for Subsurface and Ionospheric Sounding (MARSIS) on the Mars Express (MEX) spacecraft, the electron density can be measured by two methods: from the excitation of local plasma oscillations and from remote sounding. A study of the local electron density versus time for 1664 orbits revealed that in 132 orbits very sharp gradients in the electron density occurred that are similar to the ionopause boundary commonly observed at Venus. In 40 of these cases, remote sounding data have also confirmed identical locations of steep ionopause-like density gradients. Measurements from the Analyzer of Space Plasma and Energetic Atoms (ASPERA-3) Electron Spectrometer (ELS) and Ion Mass Analyzer (IMA) instruments (also on Mars Express) verify that these sharp decreases in the electron density occur somewhere between the end of the region where ionospheric photoelectrons are dominant and the magnetosheath. Combined studies of the two experiments reveal that the steep density gradients define a boundary where the magnetic fields change from open to closed. This study shows that, although the individual cases are from a wide range of altitudes, the average altitude of the boundary as a function of solar zenith angle is almost constant. The average altitude is approximately 500 km up to solar zenith angles of 60o, after which it shows a slight increase. The average thickness of the boundary is about 22 km according to remote sounding measurements. The altitude of the steep gradients shows an increase at locations with strong crustal magnetic fields.

A density gradient of VAPG peptides on a cell-resisting surface achieves selective adhesion and directional migration of smooth muscle cells over fibroblasts.

PubMed

Yu, Shan; Zuo, Xingang; Shen, Tao; Duan, Yiyuan; Mao, Zhengwei; Gao, Changyou

2018-05-01

Selective adhesion and migration of smooth muscle cells (SMCs) over fibroblasts (FIBs) is required to prevent adventitia fibrosis in vascular regeneration. In this study, a uniform cell-resisting layer of poly(ethylene glycol) (PEG) with a density gradient of azide groups was generated on a substrate by immobilizing two kinds of PEG molecules in a gradient manner. A density gradient of alkynyl-functionalized Val-Ala-Pro-Gly (VAPG) peptides was then prepared on the PEG layer via click chemistry. The VAPG density gradient was characterized by fluorescence imaging, revealing the gradual enhancement of the fluorescent intensity along the substrate direction. The adhesion and mobility of SMCs were selectively enhanced on the VAPG density gradient, leading to directional migration toward the higher peptide density (up to 84%). In contrast, the adhesion and mobility of FIBs were significantly weakened. The net displacement of SMCs also significantly increased compared with that on tissue culture polystyrene (TCPS) and that of FIBs on the gradient. The mitogen-activated protein kinase (MAPK) signaling pathways related to cell migration were studied, showing higher expressions of functional proteins from SMCs on the VAPG-modified surface in a density-dependent manner. For the first time the selective adhesion and directional migration of SMCs over FIBs was achieved by an elaborative design of a gradient surface, leading to a new insight in design of novel vascular regenerative materials. Selective cell adhesion and migration guided by regenerative biomaterials are extremely important for the regeneration of targeted tissues, which can avoid the drawbacks of incorrect and uncontrolled responses of tissue cells to implants. For example, selectivity of smooth muscle cells (SMCs) over fibroblasts (FIBs) is required to prevent adventitia fibrosis in vascular regeneration. Herein we prepare a uniform cell-repelling layer, on which SMCs-selective Val-Ala-Pro-Gly (VAPG) peptides are immobilized in a continuous manner. Selective adhesion and enhanced and directional migration of SMCs over FIBs are achieved by the interplay of cell-repelling layer and gradient SMCs-selective VAPG peptides, paving a new way for the design of novel vascular grafts with enhanced biological performance. Copyright © 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Generation of multiple toroidal dust vortices by a non-monotonic density gradient in a direct current glow discharge plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kaur, Manjit, E-mail: manjit@ipr.res.in; Bose, Sayak; Chattopadhyay, P. K.

2015-09-15

Observation of two well-separated dust vortices in an unmagnetized parallel plate DC glow discharge plasma is reported in this paper. A non-monotonic radial density profile, achieved by an especially designed cathode structure using a concentric metallic disk and ring of different radii, is observed to produce double dust tori between cathode and anode. PIV analysis of the still images of the double tori shows oppositely rotating dust structures between the central disk and the ring. Langmuir probe measurements of background plasma shows a non-uniform plasma density profile between the disk and the ring. Location and sense of rotation of themore » dust vortices coincides with the location and direction of the radial gradient in the ion drag force caused by the radial density gradient. The experimentally observed dust vorticity matches well with the calculated one using hydrodynamic formulations with shear in ion drag dominating over the dust charge gradient. These results corroborate that a radial gradient in the ion drag force directed towards cathode is the principal cause of dust rotation.« less

Preparation of synaptic plasma membrane and postsynaptic density proteins using a discontinuous sucrose gradient.

PubMed

Bermejo, Marie Kristel; Milenkovic, Marija; Salahpour, Ali; Ramsey, Amy J

2014-09-03

Neuronal subcellular fractionation techniques allow the quantification of proteins that are trafficked to and from the synapse. As originally described in the late 1960's, proteins associated with the synaptic plasma membrane can be isolated by ultracentrifugation on a sucrose density gradient. Once synaptic membranes are isolated, the macromolecular complex known as the post-synaptic density can be subsequently isolated due to its detergent insolubility. The techniques used to isolate synaptic plasma membranes and post-synaptic density proteins remain essentially the same after 40 years, and are widely used in current neuroscience research. This article details the fractionation of proteins associated with the synaptic plasma membrane and post-synaptic density using a discontinuous sucrose gradient. Resulting protein preparations are suitable for western blotting or 2D DIGE analysis.

46 CFR 164.015-2 - Types.

Code of Federal Regulations, 2010 CFR

2010-10-01

... APPROVAL MATERIALS Plastic Foam, Unicellular, Buoyant, Sheet and Molded Shape § 164.015-2 Types. (a..., polymer or copolymer plastic foam shall be of three types as follows: Type A—for life preservers, buoyant...

46 CFR 164.015-2 - Types.

Code of Federal Regulations, 2011 CFR

2011-10-01

... APPROVAL MATERIALS Plastic Foam, Unicellular, Buoyant, Sheet and Molded Shape § 164.015-2 Types. (a..., polymer or copolymer plastic foam shall be of three types as follows: Type A—for life preservers, buoyant...

46 CFR 160.060-1 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam... issue in effect on the date buoyant vests are manufacture, form a part of this subpart: Dwg. No. 160.060...

46 CFR 160.060-1 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam... issue in effect on the date buoyant vests are manufacture, form a part of this subpart: Dwg. No. 160.060...

46 CFR 160.060-1 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam... issue in effect on the date buoyant vests are manufacture, form a part of this subpart: Dwg. No. 160.060...

46 CFR 160.060-1 - Incorporation by reference.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam... issue in effect on the date buoyant vests are manufacture, form a part of this subpart: Dwg. No. 160.060...

46 CFR 160.060-5 - Construction-standard vests.

Code of Federal Regulations, 2010 CFR

2010-10-01

... arranged and distributed so as to provide the flotation characteristics and buoyancy required to hold the... drawings. (c) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as...

46 CFR 164.015-2 - Types.

Code of Federal Regulations, 2014 CFR

2014-10-01

... APPROVAL MATERIALS Plastic Foam, Unicellular, Buoyant, Sheet and Molded Shape § 164.015-2 Types. (a..., polymer or copolymer plastic foam shall be of three types as follows: Type A—for life preservers, buoyant...

Enviromental Effects on Internal Color Gradients of Early-Type Galaxies

NASA Astrophysics Data System (ADS)

La Barbera, F.; de Carvalho, R. R.; Gal, R. R.; Busarello, G.; Haines, C. P.; Mercurio, A.; Merluzzi, P.; Capaccioli, M.; Djorgovski, S. G.

2007-05-01

One of the most debated issues of observational and theoretical cosmology is that of how the environment affects the formation and evolution of galaxies. To gain new insight into this subject, we have derived surface photometry for a sample of 3,000 early-type galaxies belonging to 163 clusters with different richness, spanning a redshift range of 0.05 to 0.25. This large data-set is used to analyze how the color distribution inside galaxies depends on several parameters, such as cluster richness, local galaxy density, galaxy luminosity and redshift. We find that the internal color profile of galaxies strongly depends on the environment where galaxies reside. Galaxies in poor and rich clusters are found to follow two distinct trends in the color gradient vs. redshift diagram, with color gradients beeing less steep in rich rather than in poor clusters. No dependence of color gradients on galaxy luminosity is detected both for poor and rich clusters. We find that color gradients strongly depend on local galaxy density, with more shallow gradients in high density regions. Interestingly, this result holds only for low richness clusters, with color gradients of galaxies in rich clusters showing no dependence on local galaxy density. Our results support a reasonable picture whereby young early-type galaxies form in a dissipative collapse process, and then undergo increased (either major or minor) merging activity in richer rather than in poor clusters.

Role of density gradient driven trapped electron mode turbulence in the H-mode inner core with electron heating

DOE PAGES

Ernst, D. R.; Burrell, K. H.; Guttenfelder, W.; ...

2016-05-10

In a series of DIII-D [J. L. Luxon, Nucl. Fusion 42 614 (2002)] low torque quiescent H-mode experiments show that density gradient driven TEM (DGTEM) turbulence dominates the inner core of H-Mode plasmas during strong electron cyclotron heating (ECH). By adding 3.4 MW ECH doubles T e/T i from 0.5 to 1.0, which halves the linear DGTEM critical density gradient, locally reducing density peaking, while transport in all channels displays extreme stiffness in the density gradient. This then suggests fusion -heating may degrade inner core confinement in H-Mode plasmas with moderate density peaking and low collisionality, with equal electron andmore » ion temperatures, key conditions expected in burning plasmas. Gyrokinetic simulations using GYRO [J. Candy and R. E. Waltz, J. Comp. Phys. 186 545 (2003)] (and GENE [F. Jenko et al., Phys. Plasmas 7, 1904 (2000)]) closely match not only particle, energy, and momentum fluxes, but also density fluctuation spectra from Doppler Backscattering (DBS), with and without ECH. Inner core DBS density fluctuations display discrete frequencies with adjacent toroidal mode numbers, which we identify as DGTEMs. GS2 [W. Dorland et al., Phys. Rev. Lett. 85 5579 (2000)] predictions show the DGTEM can be suppressed, to avoid degradation with electron heating, by broadening the current density profile to attain q 0 > q min > 1.« less

46 CFR 160.047-2 - Model.

Code of Federal Regulations, 2012 CFR

2012-10-01

... APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-2 Model. Each buoyant vest specified in this subpart is a: (a) Model AK-1, adult, kapok (for...

46 CFR 160.047-2 - Model.

Code of Federal Regulations, 2011 CFR

2011-10-01

... APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-2 Model. Each buoyant vest specified in this subpart is a: (a) Model AK-1, adult, kapok (for...

46 CFR 160.047-2 - Model.

Code of Federal Regulations, 2014 CFR

2014-10-01

... APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-2 Model. Each buoyant vest specified in this subpart is a: (a) Model AK-1, adult, kapok (for...

46 CFR 160.047-2 - Model.

Code of Federal Regulations, 2013 CFR

2013-10-01

... APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Kapok or Fibrous Glass, Adult and Child § 160.047-2 Model. Each buoyant vest specified in this subpart is a: (a) Model AK-1, adult, kapok (for...

Magmatic densities control erupted volumes in Icelandic volcanic systems

NASA Astrophysics Data System (ADS)

Hartley, Margaret; Maclennan, John

2018-04-01

Magmatic density and viscosity exert fundamental controls on the eruptibility of magmas. In this study, we investigate the extent to which magmatic physical properties control the eruptibility of magmas from Iceland's Northern Volcanic Zone (NVZ). By studying subaerial flows of known age and volume, we are able to directly relate erupted volumes to magmatic physical properties, a task that has been near-impossible when dealing with submarine samples dredged from mid-ocean ridges. We find a strong correlation between magmatic density and observed erupted volumes on the NVZ. Over 85% of the total volume of erupted material lies close to a density and viscosity minimum that corresponds to the composition of basalts at the arrival of plagioclase on the liquidus. These magmas are buoyant with respect to the Icelandic upper crust. However, a number of small-volume eruptions with densities greater than typical Icelandic upper crust are also found in Iceland's neovolcanic zones. We use a simple numerical model to demonstrate that the eruption of magmas with higher densities and viscosities is facilitated by the generation of overpressure in magma chambers in the lower crust and uppermost mantle. This conclusion is in agreement with petrological constraints on the depths of crystallisation under Iceland.

The Relationships Between ELM Suppression, Pedestal Profiles, and Lithium Wall Coatings in NSTX

DOE Office of Scientific and Technical Information (OSTI.GOV)

D.P. Boyle, R. Maingi, P.B. Snyder, J. Manickam, T.H. Osborne, R.E. Bell, B.P. LeBlanc, and the NSTX Team

2012-08-17

Recently in the National Spherical Torus Experiment (NSTX), increasing lithium wall coatings suppressed edge localized modes (ELMs), gradually but not quite monotonically. This work details profile and stability analysis as ELMs disappeared throughout the lithium scan. While the quantity of lithium deposited between discharges did not uniquely determine the presence of ELMs, profile analysis demonstrated that lithium was correlated to wider density and pressure pedestals with peak gradients farther from the separatrix. Moreover, the ELMy and ELM-free discharges were cleanly separated by their density and pedestal widths and peak gradient locations. Ultimately, ELMs were only suppressed when lithium caused themore » density pedestal to widen and shift inward. These changes in the density gradient were directly reflected in the pressure gradient and calculated bootstrap current. This supports the theory that ELMs in NSTX are caused by peeling and/or ballooning modes, as kink/peeling modes are stabilized when the edge current and pressure gradient shift away from the separatrix. Edge stability analysis using ELITE corroborated this picture, as reconstructed equilibria from ELM-free discharges were generally farther from their kink/peeling stability boundaries than ELMy discharges. We conclude that density profile control provided by lithium is the key first step to ELM suppression in NSTX« less

The relationships between edge localized modes suppression, pedestal profiles and lithium wall coatings in NSTX

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boyle, D. P.; Maingi, R.; Snyder, P. B.

2011-01-01

Recently in the National Spherical Torus Experiment (NSTX), increasing lithium wall coatings suppressed edge localized modes (ELMs), gradually but not quite monotonically. This work details profile and stability analysis as ELMs disappeared throughout the lithium scan. While the quantity of lithium deposited between discharges did not uniquely determine the presence of ELMs, profile analysis demonstrated that lithium was correlated with wider density and pressure pedestals with peak gradients farther from the separatrix. Moreover, the ELMy and ELM-free discharges were cleanly separated by their density and pedestal widths and peak gradient locations. Ultimately, ELMs were only suppressed when lithium caused themore » density pedestal to widen and shift inward. These changes in the density gradient were directly reflected in the pressure gradient and calculated bootstrap current. This supports the theory that ELMs in NSTX are caused by peeling and/or ballooning modes, as kink/peeling modes are stabilized when the edge current and pressure gradient shift away from the separatrix. Edge stability analysis using ELITE corroborated this picture, as reconstructed equilibria from ELM-free discharges were generally farther from their kink/peeling stability boundaries than ELMy discharges. We conclude that density profile control provided by lithium is the key first step to ELM suppression in NSTX.« less

Testing of a new dense gas approach in the Lagrangian Dispersion Model SPRAY.

NASA Astrophysics Data System (ADS)

Mortarini, Luca; Alessandrini, Stefano; Ferrero, Enrico; Anfossi, Domenico; Manfrin, Massimiliano

2013-04-01

A new original method for the dispersion of a positively and negatively buoyant plume is proposed. The buoyant pollutant movement is treated introducing a fictitious scalar inside the Lagrangian Stochastic Particle Model SPRAY. The method is based on the same idea of Alessandrini and Ferrero (Phys. A 388:1375-1387, 2009) for the treatment of a background substance entrainment into the plume. In this application, the fictitious scalar is the density and momentum difference between the plume portions and the environment air that naturally takes into account the interaction between the plume and the environment. As a consequence, no more particles than those inside the plume have to be released to simulate the entrainment of the background air temperature. In this way the entrainment is properly simulated and the plume sink is calculated from the local property of the flow. This new approach is wholly Lagrangian in the sense that the Eulerian grid is only used to compute the propriety of a portion of the plume from the particles contained in every cell. No equation of the bulk plume is solved on a fixed grid. To thoroughly test the turbulent velocity field calculated by the model, the latter is compared with a water tank experiment carried out in the TURLAB laboratory in Turin (Italy). A vertical density driven current was created releasing a saline solution (salt and water) in a water tank with no mean flow. The experiment reproduces in physical similarity, based on the density Froud number, the release of a dense gas in the planetary boundary layer and the Particle Image Velocimetry technique has been used to analyze the buoyancy generated velocity field. The high temporal and spatial resolution of the measurements gives a deep insight to the problems of the bouncing of the dense gas and of the creation of the outflow velocity at the ground.

Complex mountain terrain and disturbance history drive variation in forest aboveground live carbon density in the western Oregon Cascades, USA

Treesearch

Harold S.J. Zald; Thomas A. Spies; Rupert Seidl; Robert J. Pabst; Keith A. Olsen; Ashley Steel

2016-01-01

Forest carbon (C) density varies tremendously across space due to the inherent heterogeneity of forest ecosystems. Variation of forest C density is especially pronounced in mountainous terrain, where environmental gradients are compressed and vary at multiple spatial scales. Additionally, the influence of environmental gradients may vary with forest age and...

Controls on stand transpiration and soil water utilization along a tree density gradient in a Neotropical savanna

Treesearch

Sandra J. Bucci; Fabian G. Scholz; Guillermo Goldstein; William A. Hoffmann; Frederick C. Meinzer; Augusto C. Franco; Thomas Giambelluca; Fernando Miralles-Wilhelm

2008-01-01

Environmental controls of stand-level tree transpiration (E) and seasonal patterns of soil water utilization were studied in five central Brazilian savanna (Cerrado) sites differing in tree density. Tree density of Cerrado vegetation in the study area consistently changes along topographic gradients from ~1,000 trees ha-1 in open savannas (campo...

The divergent fates of primitive hydrospheric water on Earth and Mars

NASA Astrophysics Data System (ADS)

Wade, Jon; Dyck, Brendan; Palin, Richard M.; Moore, James D. P.; Smye, Andrew J.

2017-12-01

Despite active transport into Earth’s mantle, water has been present on our planet’s surface for most of geological time. Yet water disappeared from the Martian surface soon after its formation. Although some of the water on Mars was lost to space via photolysis following the collapse of the planet’s magnetic field, the widespread serpentinization of Martian crust suggests that metamorphic hydration reactions played a critical part in the sequestration of the crust. Here we quantify the relative volumes of water that could be removed from each planet’s surface via the burial and metamorphism of hydrated mafic crusts, and calculate mineral transition-induced bulk-density changes at conditions of elevated pressure and temperature for each. The metamorphic mineral assemblages in relatively FeO-rich Martian lavas can hold about 25 per cent more structurally bound water than those in metamorphosed terrestrial basalts, and can retain it at greater depths within Mars. Our calculations suggest that in excess of 9 per cent by volume of the Martian mantle may contain hydrous mineral species as a consequence of surface reactions, compared to about 4 per cent by volume of Earth’s mantle. Furthermore, neither primitive nor evolved hydrated Martian crust show noticeably different bulk densities compared to their anhydrous equivalents, in contrast to hydrous mafic terrestrial crust, which transforms to denser eclogite upon dehydration. This would have allowed efficient overplating and burial of early Martian crust in a stagnant-lid tectonic regime, in which the lithosphere comprised a single tectonic plate, with only the warmer, lower crust involved in mantle convection. This provided an important sink for hydrospheric water and a mechanism for oxidizing the Martian mantle. Conversely, relatively buoyant mafic crust and hotter geothermal gradients on Earth reduced the potential for upper-mantle hydration early in its geological history, leading to water being retained close to its surface, and thus creating conditions conducive for the evolution of complex multicellular life.

The divergent fates of primitive hydrospheric water on Earth and Mars.

PubMed

Wade, Jon; Dyck, Brendan; Palin, Richard M; Moore, James D P; Smye, Andrew J

2017-12-20

Despite active transport into Earth's mantle, water has been present on our planet's surface for most of geological time. Yet water disappeared from the Martian surface soon after its formation. Although some of the water on Mars was lost to space via photolysis following the collapse of the planet's magnetic field, the widespread serpentinization of Martian crust suggests that metamorphic hydration reactions played a critical part in the sequestration of the crust. Here we quantify the relative volumes of water that could be removed from each planet's surface via the burial and metamorphism of hydrated mafic crusts, and calculate mineral transition-induced bulk-density changes at conditions of elevated pressure and temperature for each. The metamorphic mineral assemblages in relatively FeO-rich Martian lavas can hold about 25 per cent more structurally bound water than those in metamorphosed terrestrial basalts, and can retain it at greater depths within Mars. Our calculations suggest that in excess of 9 per cent by volume of the Martian mantle may contain hydrous mineral species as a consequence of surface reactions, compared to about 4 per cent by volume of Earth's mantle. Furthermore, neither primitive nor evolved hydrated Martian crust show noticeably different bulk densities compared to their anhydrous equivalents, in contrast to hydrous mafic terrestrial crust, which transforms to denser eclogite upon dehydration. This would have allowed efficient overplating and burial of early Martian crust in a stagnant-lid tectonic regime, in which the lithosphere comprised a single tectonic plate, with only the warmer, lower crust involved in mantle convection. This provided an important sink for hydrospheric water and a mechanism for oxidizing the Martian mantle. Conversely, relatively buoyant mafic crust and hotter geothermal gradients on Earth reduced the potential for upper-mantle hydration early in its geological history, leading to water being retained close to its surface, and thus creating conditions conducive for the evolution of complex multicellular life.

Porcine reproductive and respiratory syndrome virus: morphological, biochemical and serological characteristics of Quebec isolates associated with acute and chronic outbreaks of porcine reproductive and respiratory syndrome.

PubMed Central

Mardassi, H; Athanassious, R; Mounir, S; Dea, S

1994-01-01

Cytolytic and noncytolytic strains of the porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in primary cultures of porcine alveolar macrophages (PAM) from lung homogenates of stillborn fetuses or blood samples of dyspneic piglets collected from Quebec pig farms having experienced acute or chronic outbreaks of PRRS. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. Intracytoplasmic enveloped viral particles that tended to accumulate into cytoplasmic vesicles were observed in the infected PAM; no budding was demonstrated at the level of the cytoplasmic membrane. The extracellular virions appeared as pleomorphic but mostly spherical enveloped particles, 50-72 nm in diameter (averaged diameter of 50 particles was 58.3 nm), with an isometric core about 25-30 nm. Buoyant density of the virus in CsCL density gradients was estimated to 1.18-1.20 g/mL. No hemagglutinating activity was demonstrated. Analysis of semipurified virions of isolate IAF-exp91 by radioimmunoprecipitation (RIPA) and Western immunoblotting experiments, using reference rabbit and porcine hyperimmune sera, revealed four major viral proteins, a predominant 15 kD N protein and three other proteins with predicted M(r_ of 19, 26 and 42 kD. Progeny viral particles produced in PRRSV-infected PAM in the presence of tunicamycin lacked the 42 kD protein, thus confirming its N-glycosylated nature. Immunoprecipitation experiments using the anti-ATCC-VR2332 MoAbs confirmed the close antigenic relationships between Quebec and American reference isolates of PRRSV. Images Fig. 1. Fig. 2. Fig. 3. Fig. 4. Fig. 5. Fig. 6. PMID:8143254

Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning

PubMed Central

Walsby, Anthony E; Holland, Daryl P

2005-01-01

Two particular difficulties in measuring the sinking velocities of phytoplankton cells are preventing convection within the sedimenting medium and determining the changing depth of the cells. These problems are overcome by using a density-stabilized sedimentation column scanned by a laser. For freshwater species, a suspension of phytoplankton is layered over a vertical density gradient of Percoll solution; as the cells sink down the column their relative concentration is measured by the forward scattering of light from a laser beam that repeatedly scans up and down the column. The Percoll gradient stabilizes the column, preventing vertical mixing by convection, radiation or perturbation of density by the descending cells. Measurements were made on suspensions of 15 μm polystyrene microspheres with a density of 1050 kg m−3; the mean velocity was 6.28 μm s−1, within 1.5% of that calculated by the Stokes equation, 6.36 μm s−1. Measurements made on the filamentous cyanobacterium Planktothrix rubescens gave mean velocities within the theoretical range of values based on the range of size, shape, orientation and density of the particles in a modified Stokes equation. Measurements on marine phytoplankton may require density gradients prepared with other substances. PMID:16849271

Smoke-Point Properties of Non-Buoyant Round Laminar Jet Diffusion Flames. Appendix J

NASA Technical Reports Server (NTRS)

Urban, D. L.; Yuan, Z.-G.; Sunderland, P. B.; Lin, K.-C.; Dai, Z.; Faeth, G. M.

2000-01-01

The laminar smoke-point properties of non-buoyant round laminar jet diffusion flames were studied emphasizing results from long-duration (100-230 s) experiments at microgravity carried out in orbit aboard the space shuttle Columbia. Experimental conditions included ethylene- and propane-fueled flames burning in still air at an ambient temperature of 300 K, pressures of 35-130 kPa, jet exit diameters of 1.6 and 2.7 mm, jet exit velocities of 170-690 mm/s, jet exit Reynolds numbers of 46-172, characteristic flame residence times of 40-302 ms, and luminous flame lengths of 15-63 mm. Contrary to the normal-gravity laminar smoke point, in microgravity, the onset of laminar smoke-point conditions involved two flame configurations: closed-tip flames with soot emissions along the flame axis and open-tip flames with soot emissions from an annular ring about the flame axis. Open-tip flames were observed at large characteristic flame residence times with the onset of soot emissions associated with radiative quenching near the flame tip: nevertheless, unified correlations of laminar smoke-point properties were obtained that included both flame configurations. Flame lengths at laminar smoke-point conditions were well correlated in terms of a corrected fuel flow rate suggested by a simplified analysis of flame shape. The present steady and non-buoyant flames emitted soot more readily than non-buoyant flames in earlier tests using ground-based microgravity facilities and than buoyant flames at normal gravity, as a result of reduced effects of unsteadiness, flame disturbances, and buoyant motion. For example, present measurements of laminar smoke-point flame lengths at comparable conditions were up to 2.3 times shorter than ground-based microgravity measurements and up to 6.4 times shorter than buoyant flame measurements. Finally, present laminar smoke-point flame lengths were roughly inversely proportional to pressure to a degree that is a somewhat smaller than observed during earlier tests both at microgravity (using ground-based facilities) and at normal gravity.

Thymocyte plasma membrane of the rainbow trout, Salmo gairdneri: Associated immunoglobulin and heteroantigens

USGS Publications Warehouse

Warr, G.W.; DeLuca, D.; Anderson, D.P.

1983-01-01

1. Thymic lymphocytes of the rainbow trout, S. gairdneri were disrupted and a plasma membrane containing fraction isolated by differential and buoyant density centrifugation.2. Radioiodine introduced into the membrane by the lactoperoxidase catalyzed reaction and immunoglobulin (identified by radioimmunoassay with monoclonal antibody) both copurified in the plasma membrane fraction.3. Rabbit antibody raised to the plasma membrane fraction showed a strong reaction with trout lymphocytes in immunofluorescence, was mitogenic for trout lymphocytes, and recognized lymphocyte membrane heteroantigens of molecular weight > 70,000 in the thymus and 45,000–95,000 in the head kidney.

Airships for transporting highly volatile commodities

NASA Technical Reports Server (NTRS)

Sonstegaard, M.

1975-01-01

Large airships may prove feasible as carriers of commodities that move as gases or cryogenic liquids; buoyant gaseous cargo could be ballasted with liquid cargo. Airships are compact in shape, operate in a rarified medium, and hence can be fast and perhaps economic carriers of costly cryogenic tanks. The high-pressure gas pipeline has excessive surface area when carrying hydrogen and excessive fluid density when carrying natural gas, while the cryogenic ocean tanker runs in a dense medium and makes gravity waves. But the airship, despite its fluid dynamic advantages, faces problems of safety, weather, and altitude control.

Sorting process of nanoparticles and applications of same

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tyler, Timothy P.; Henry, Anne-Isabelle; Van Duyne, Richard P.

In one aspect of the present invention, a method for sorting nanoparticles includes preparing a high-viscosity density gradient medium filled in a container, dispersing nanoparticles into an aqueous solution to form a suspension of the nanoparticles, each nanoparticle having one or more cores and a shell encapsulating the one or more cores, layering the suspension of the nanoparticles on the top of the high-viscosity density gradient medium in the container, and centrifugating the layered suspension of the nanoparticles on the top of the high-viscosity density gradient medium in the container at a predetermined speed for a predetermined period of timemore » to form a gradient of fractions of the nanoparticles along the container, where each fraction comprises nanoparticles in a respective one of aggregation states of the nanoparticles.« less

A cross-sectional analysis of the relationship between tobacco and alcohol outlet density and neighbourhood deprivation.

PubMed

Shortt, Niamh K; Tisch, Catherine; Pearce, Jamie; Mitchell, Richard; Richardson, Elizabeth A; Hill, Sarah; Collin, Jeff

2015-10-05

There is a strong socio-economic gradient in both tobacco-and alcohol-related harm. One possible factor contributing to this social gradient may be greater availability of tobacco and alcohol in more socially-deprived areas. A higher density of tobacco and alcohol outlets is not only likely to increase supply but also to raise awareness of tobacco/alcohol brands, create a competitive local market that reduces product costs, and influence local social norms relating to tobacco and alcohol consumption. This paper examines the association between the density of alcohol and tobacco outlets and neighbourhood-level income deprivation. Using a national tobacco retailer register and alcohol licensing data this paper calculates the density of alcohol and tobacco retail outlets per 10,000 population for small neighbourhoods across the whole of Scotland. Average outlet density was calculated for neighbourhoods grouped by their level of income deprivation. Associations between outlet density and deprivation were analysed using one way analysis of variance. There was a positive linear relationship between neighbourhood deprivation and outlets for both tobacco (p <0.001) and off-sales alcohol (p <0.001); the most deprived quintile of neighbourhoods had the highest densities of both. In contrast, the least deprived quintile had the lowest density of tobacco and both off-sales and on-sales alcohol outlets. The social gradient evident in alcohol and tobacco supply may be a contributing factor to the social gradient in alcohol- and tobacco-related disease. Policymakers should consider such gradients when creating tobacco and alcohol control policies. The potential contribution to public health, and health inequalities, of reducing the physical availability of both alcohol and tobacco products should be examined in developing broader supply-side interventions.

Analytic energy gradients for the coupled-cluster singles and doubles method with the density-fitting approximation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bozkaya, Uğur, E-mail: ugur.bozkaya@hacettepe.edu.tr; Department of Chemistry, Atatürk University, Erzurum 25240; Sherrill, C. David

2016-05-07

An efficient implementation is presented for analytic gradients of the coupled-cluster singles and doubles (CCSD) method with the density-fitting approximation, denoted DF-CCSD. Frozen core terms are also included. When applied to a set of alkanes, the DF-CCSD analytic gradients are significantly accelerated compared to conventional CCSD for larger molecules. The efficiency of our DF-CCSD algorithm arises from the acceleration of several different terms, which are designated as the “gradient terms”: computation of particle density matrices (PDMs), generalized Fock-matrix (GFM), solution of the Z-vector equation, formation of the relaxed PDMs and GFM, back-transformation of PDMs and GFM to the atomic orbitalmore » (AO) basis, and evaluation of gradients in the AO basis. For the largest member of the alkane set (C{sub 10}H{sub 22}), the computational times for the gradient terms (with the cc-pVTZ basis set) are 2582.6 (CCSD) and 310.7 (DF-CCSD) min, respectively, a speed up of more than 8-folds. For gradient related terms, the DF approach avoids the usage of four-index electron repulsion integrals. Based on our previous study [U. Bozkaya, J. Chem. Phys. 141, 124108 (2014)], our formalism completely avoids construction or storage of the 4-index two-particle density matrix (TPDM), using instead 2- and 3-index TPDMs. The DF approach introduces negligible errors for equilibrium bond lengths and harmonic vibrational frequencies.« less

46 CFR 160.060-5 - Construction-standard vests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... drawings. (c) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as.... Two pieces of fabric shall be cut to the pattern shown on Dwg. No. 160.060-1, Sheet 1 for the adult...

46 CFR 160.060-5 - Construction-standard vests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... drawings. (c) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as.... Two pieces of fabric shall be cut to the pattern shown on Dwg. No. 160.060-1, Sheet 1 for the adult...

46 CFR 160.060-5 - Construction-standard vests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... drawings. (c) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as.... Two pieces of fabric shall be cut to the pattern shown on Dwg. No. 160.060-1, Sheet 1 for the adult...

46 CFR 160.060-5 - Construction-standard vests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... drawings. (c) Buoyant inserts. The unicellular plastic foam buoyant inserts shall be cut and formed as.... Two pieces of fabric shall be cut to the pattern shown on Dwg. No. 160.060-1, Sheet 1 for the adult...

46 CFR 160.010-4 - General requirements for buoyant apparatus.

Code of Federal Regulations, 2014 CFR

2014-10-01

... light twine. (h) Each peripheral body type buoyant apparatus without a net or platform on the inside... pigmented in a dark color. A typical method of securing lifelines and pendants to straps of webbing is shown...

46 CFR 160.010-4 - General requirements for buoyant apparatus.

Code of Federal Regulations, 2013 CFR

2013-10-01

... light twine. (h) Each peripheral body type buoyant apparatus without a net or platform on the inside... pigmented in a dark color. A typical method of securing lifelines and pendants to straps of webbing is shown...

46 CFR 160.010-4 - General requirements for buoyant apparatus.

Code of Federal Regulations, 2012 CFR

2012-10-01

... light twine. (h) Each peripheral body type buoyant apparatus without a net or platform on the inside... pigmented in a dark color. A typical method of securing lifelines and pendants to straps of webbing is shown...

Low-Temperature Oxidation Reactions and Cool Flames at Earth and Reduced Gravity

NASA Technical Reports Server (NTRS)

Pearlman, Howard

1999-01-01

Non-isothermal studies of cool flames and low temperature oxidation reactions in unstirred closed vessels are complicated by the perturbing effects of natural convection at earth gravity. Buoyant convection due to self-heating during the course of slow reaction produces spatio-temporal variations in the thermal and thus specie concentration fields due to the Arrhenius temperature dependence of the reaction rates. Such complexities have never been quantitatively modeled and were the primary impetus for the development of CSTR's (continuously stirred tank reactors) 30 years ago. While CSTR's have been widely adopted since they offer the advantage of spatial uniformity in temperature and concentration, all gradients are necessarily destroyed along with any structure that may otherwise develop. Microgravity offers a unique environment where buoyant convection can be effectively minimized and the need for stirring eliminated. Moreover, eliminating buoyancy and the need for stirring eliminates complications associated with the induced hydrodynamic field whose influence on heat transport and hot spot formation, hence explosion limits, is not fully realized. The objective of this research is to quantitatively determine and understand the fundamental mechanisms that control the onset and evolution of low temperature reactions and cool flames in both static and flow reactors. Microgravity experiments will be conducted to obtain benchmark data on the structure (spatio-temporal temperature, concentration, flow fields), the dynamics of the chemical fronts, and the ignition diagrams (pressure vs. temperature). Ground-based experiments will be conducted to ascertain the role of buoyancy. Numerical simulations including detailed kinetics will be conducted and compared to experiment.

Fully kinetic Biermann battery and associated generation of pressure anisotropy

NASA Astrophysics Data System (ADS)

Schoeffler, K. M.; Loureiro, N. F.; Silva, L. O.

2018-03-01

The dynamical evolution of a fully kinetic, collisionless system with imposed background density and temperature gradients is investigated analytically. The temperature gradient leads to the generation of temperature anisotropy, with the temperature along the gradient becoming larger than that in the direction perpendicular to it. This causes the system to become unstable to pressure anisotropy driven instabilities, dominantly to the electron Weibel instability. When both density and temperature gradients are present and nonparallel to each other, we obtain a Biermann-like linear-in-time magnetic field growth. Accompanying particle-in-cell numerical simulations are shown to confirm our analytical results.

Is the 'Fast Halo' around Hawaii as imaged in the PLUME experiment direct evidence for buoyant plume-fed asthenosphere?

NASA Astrophysics Data System (ADS)

Morgan, J. P.; Shi, C.; Hasenclever, J.

2010-12-01

An intriguing spatial pattern of variations in shear-wave arrival times has been mapped in the PLUME ocean bottom experiment (Wolfe et al., 2009) around Hawaii. The pattern consists of a halo of fast travel times surrounding a disk of slow arrivals from waves traveling up though the plume. We think it is directly sensing the pattern of dynamic uplift of the base of a buoyant asthenosphere - the buoyancy of the plume conduit lifting a 'rim' of the cooler, denser mantle that the plume rises through. The PLUME analysis inverted for lateral shear velocity variations beneath the lithosphere, after removing the assumed 1-D model velocity structure IASP91. They found that a slow plume-conduit extends to at least 1200 km below the Hawaiian hotspot. In this inversion the slow plume conduit is — quite surprisingly - surrounded by a fast wavespeed halo. A fast halo is impossible to explain as a thermal halo around the plume; this should lead to a slow wavespeed halo, not a fast one. Plume-related shearwave anisotropy also cannot simply explain this pattern — simple vertical strain around the plume conduit would result in an anisotropic slow shear-wavespeed halo, not a fast one. (Note the PLUME experiment’s uniform ‘fast-halo’ structure from 50-400km is likely to have strong vertical streaking in the seismic image; Pacific Plate-driven shear across a low-viscosity asthenosphere would be expected to disrupt and distort any cold sheet of vertical downwelling structure between 50-400km depths so that it would no longer be vertical as it is in the 2009 PLUME image with its extremely poor vertical depth control.) If the asthenosphere is plume-fed, hence more buoyant than underlying mantle, then there can be a simple explanation for this pattern. The anomaly would be due to faster traveltimes resulting from dynamic relief at the asthenosphere-mesosphere interface; uplift of the denser mesosphere by the buoyancy of the rising plume increases the distance a wave travels through faster mantle and reduces the distance though the slower asthenosphere. With this interpretation, the inference of a radially symmetric ~40-70 km high-~250 km-radius ‘bump’ of uplift of the base of buoyant plume-fed asthenosphere (PFA) can be directly estimated from PLUME results and the measured ~6-10% reduction in shear velocity between the PFA and underlying mantle. The inferred dynamic relief at the base of the PFA due to buoyancy within the underlying plume conduit is strikingly similar to the relief we find in recent axisymmetric 2D and Cartesian 3-D numerical experiments that explore the dynamics of mantle convection with a PFA. The width and height of the bump scale directly with the total buoyancy anomaly in the upper ~500km of the plume conduit, we discuss numerical experiments that quantify this relationship, show that it is, to first order, independent of the viscosity of material in the plume conduit or asthenosphere, and which also quantify the ~400km-radius geoid anomaly produced by these subasthenospheric mantle density anomalies. This effect can only happen if the asthenosphere is more buoyant than underlying mantle — and is therefore direct evidence that a buoyant plume-fed asthenosphere exists around Hawaii.

Generation of a wakefield undulator in plasma with transverse density gradient

DOE PAGES

Stupakov, Gennady V.

2017-11-30

Here, we show that a short relativistic electron beam propagating in a plasma with a density gradient perpendicular to the direction of motion generates a wakefield in which a witness bunch experiences a transverse force. A density gradient oscillating along the beam path would create a periodically varying force$-$an undulator, with an estimated strength of the equivalent magnetic field more than ten Tesla. This opens an avenue for creation of a high-strength, short-period undulators, which eventually may lead to all-plasma, free electron lasers where a plasma wakefield acceleration is naturally combined with a plasma undulator in a unifying, compact setup.

Generation of a wakefield undulator in plasma with transverse density gradient

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stupakov, Gennady V.

Here, we show that a short relativistic electron beam propagating in a plasma with a density gradient perpendicular to the direction of motion generates a wakefield in which a witness bunch experiences a transverse force. A density gradient oscillating along the beam path would create a periodically varying force$-$an undulator, with an estimated strength of the equivalent magnetic field more than ten Tesla. This opens an avenue for creation of a high-strength, short-period undulators, which eventually may lead to all-plasma, free electron lasers where a plasma wakefield acceleration is naturally combined with a plasma undulator in a unifying, compact setup.

Plasticity in skeletal characteristics of nursery-raised staghorn coral, Acropora cervicornis

USGS Publications Warehouse

Kuffner, Ilsa B.; Bartels, Erich; Stathakopoulos, Anastasios; Enochs, Ian C.; Kolodziej, Graham; Toth, Lauren; Manzello, Derek P.

2017-01-01

Staghorn coral, Acropora cervicornis, is a threatened species and the primary focus of western Atlantic reef restoration efforts to date. We compared linear extension, calcification rate, and skeletal density of nursery-raised A. cervicornis branches reared for 6 months either on blocks attached to substratum or hanging from PVC trees in the water column. We demonstrate that branches grown on the substratum had significantly higher skeletal density, measured using computerized tomography, and lower linear extension rates compared to water-column fragments. Calcification rates determined with buoyant weighing were not statistically different between the two grow-out methods, but did vary among coral genotypes. Whereas skeletal density and extension rates were plastic traits that depended on grow-out method, calcification rate was conserved. Our results show that the two rearing methods generate the same amount of calcium carbonate skeleton but produce colonies with different skeletal characteristics and suggest that there is genetically based variability in coral calcification performance.

The dynamics of oceanic fronts. I - The Gulf Stream

NASA Technical Reports Server (NTRS)

Kao, T. W.

1980-01-01

The establishment and maintenance of the mean hydrographic properties of large-scale density fronts in the upper ocean is considered. The dynamics is studied by posing an initial value problem starting with a near-surface discharge of buoyant water with a prescribed density deficit into an ambient stationary fluid of uniform density; full time dependent diffusion and Navier-Stokes equations are then used with constant eddy diffusion and viscosity coefficients, together with a constant Coriolis parameter. Scaling analysis reveals three independent scales of the problem including the radius of deformation of the inertial length, buoyancy length, and diffusive length scales. The governing equations are then suitably scaled and the resulting normalized equations are shown to depend on the Ekman number alone for problems of oceanic interest. It is concluded that the mean Gulf Stream dynamics can be interpreted in terms of a solution of the Navier-Stokes and diffusion equations, with the cross-stream circulation responsible for the maintenance of the front; this mechanism is suggested for the maintenance of the Gulf Stream dynamics.

Plasticity in skeletal characteristics of nursery-raised staghorn coral, Acropora cervicornis

NASA Astrophysics Data System (ADS)

Kuffner, Ilsa B.; Bartels, Erich; Stathakopoulos, Anastasios; Enochs, Ian C.; Kolodziej, G.; Toth, Lauren T.; Manzello, Derek P.

2017-09-01

Staghorn coral, Acropora cervicornis, is a threatened species and the primary focus of western Atlantic reef restoration efforts to date. We compared linear extension, calcification rate, and skeletal density of nursery-raised A. cervicornis branches reared for 6 months either on blocks attached to substratum or hanging from PVC trees in the water column. We demonstrate that branches grown on the substratum had significantly higher skeletal density, measured using computerized tomography, and lower linear extension rates compared to water-column fragments. Calcification rates determined with buoyant weighing were not statistically different between the two grow-out methods, but did vary among coral genotypes. Whereas skeletal density and extension rates were plastic traits that depended on grow-out method, calcification rate was conserved. Our results show that the two rearing methods generate the same amount of calcium carbonate skeleton but produce colonies with different skeletal characteristics and suggest that there is genetically based variability in coral calcification performance.

BATHYMETRIC IRREGULARITIES, JET FORMATION, AND SUBSEQUENT MIXING PROCESSES

EPA Science Inventory

It is well known that bathymetric contours influence and steer currents and that irregularities in bathymetry contribute to the formation of aquatic non-buoyant jets and buoyant plumes. For example, bathymetric irregularities can channel flow through canyons or accelerate flow ov...

Flow-field characteristics of high-temperature annular buoyant jets and their development laws influenced by ventilation system.

PubMed

Wang, Yi; Huang, Yanqiu; Liu, Jiaping; Wang, Hai; Liu, Qiuhan

2013-01-01

The flow-field characteristics of high-temperature annular buoyant jets as well as the development laws influenced by ventilation system were studied using numerical methods to eliminate the pollutants effectively in this paper. The development laws of high-temperature annular buoyant jets were analyzed and compared with previous studies, including radial velocity distribution, axial velocity and temperature decay, reattachment position, cross-section diameter, volumetric flow rate, and velocity field characteristics with different pressures at the exhaust hood inlet. The results showed that when the ratio of outer diameter to inner diameter of the annulus was smaller than 5/2, the flow-field characteristics had significant difference compared to circular buoyant jets with the same outer diameter. For similar diameter ratios, reattachment in this paper occurred further downstream in contrast to previous study. Besides, the development laws of volumetric flow rate and cross-section diameter were given with different initial parameters. In addition, through analyzing air distribution characteristics under the coupling effect of high-temperature annular buoyant jets and ventilation system, it could be found that the position where maximum axial velocity occurred was changing gradually when the pressure at the exhaust hood inlet changed from 0 Pa to -5 Pa.

Flow-Field Characteristics of High-Temperature Annular Buoyant Jets and Their Development Laws Influenced by Ventilation System

PubMed Central

Liu, Jiaping; Wang, Hai; Liu, Qiuhan

2013-01-01

The flow-field characteristics of high-temperature annular buoyant jets as well as the development laws influenced by ventilation system were studied using numerical methods to eliminate the pollutants effectively in this paper. The development laws of high-temperature annular buoyant jets were analyzed and compared with previous studies, including radial velocity distribution, axial velocity and temperature decay, reattachment position, cross-section diameter, volumetric flow rate, and velocity field characteristics with different pressures at the exhaust hood inlet. The results showed that when the ratio of outer diameter to inner diameter of the annulus was smaller than 5/2, the flow-field characteristics had significant difference compared to circular buoyant jets with the same outer diameter. For similar diameter ratios, reattachment in this paper occurred further downstream in contrast to previous study. Besides, the development laws of volumetric flow rate and cross-section diameter were given with different initial parameters. In addition, through analyzing air distribution characteristics under the coupling effect of high-temperature annular buoyant jets and ventilation system, it could be found that the position where maximum axial velocity occurred was changing gradually when the pressure at the exhaust hood inlet changed from 0 Pa to −5 Pa. PMID:24000278

The gravitational extension in the Central Range of Taiwan induced by the instability of intrinsic buoyancy

NASA Astrophysics Data System (ADS)

Lo, C.; Kuo-Chen, H.; Hsu, S.

2013-12-01

The active Taiwan orogen is situated in the tectonic convergence between the Philippine Sea plate and Eurasian passive margin. The thick crust under the Central Range of Taiwan was demonstrated by the results from the TAIGER project during 2004-2009. The results show that the deepest moho (~60 km thickness) is located at the eastern flank of the Central Range, while the averaged crust thickness is over 50 km beneath the whole mountain ranges from south to north. Physically the thickened crust provides an excess of the gravitational potential energy (GPE) with respect to the vicinity, implying that the Central Range itself behaves intrinsic extension stress environment. However, due to limited geophysical information such a phenomenon was not well evaluated and not considered to be one of the important factors for the Taiwan mountain building process. In this study, we calculate the GPE of the whole Taiwan region from recent Vp tomography via seismic velocity-rock density empirical relationship. From the catalogue of the earthquake focal mechanisms of Broadband Array in Taiwan for Seismology (BATS), a quite number of extensional earthquakes are distributed in the 10-40 km deep in and around the Central Range, where the crustal potential energy is distinctively higher. Besides, the principal axes of these extensional earthquakes are mainly normal to the large gradient of crust ΔGPE at the edge of Central Range. Accordingly, we conclude that the Central Range is undergoing the mountain building by the strong plate collision; meanwhile it is also bearing the gravitationally instable extension due to inherent buoyant thickening crust.

Active ammonia oxidizers in an acidic soil are phylogenetically closely related to neutrophilic archaeon.

PubMed

Wang, Baozhan; Zheng, Yan; Huang, Rong; Zhou, Xue; Wang, Dongmei; He, Yuanqiu; Jia, Zhongjun

2014-03-01

All cultivated ammonia-oxidizing archaea (AOA) within the Nitrososphaera cluster (former soil group 1.1b) are neutrophilic. Molecular surveys also indicate the existence of Nitrososphaera-like phylotypes in acidic soil, but their ecological roles are poorly understood. In this study, we present molecular evidence for the chemolithoautotrophic growth of Nitrososphaera-like AOA in an acidic soil with pH 4.92 using DNA-based stable isotope probing (SIP). Soil microcosm incubations demonstrated that nitrification was stimulated by urea fertilization and accompanied by a significant increase in the abundance of AOA rather than ammonia-oxidizing bacteria (AOB). Real-time PCR analysis of amoA genes as a function of the buoyant density of the DNA gradient following the ultracentrifugation of the total DNA extracted from SIP microcosms indicated a substantial growth of soil AOA during nitrification. Pyrosequencing of the total 16S rRNA genes in the "heavy" DNA fractions suggested that archaeal communities were labeled to a much greater extent than soil AOB. Acetylene inhibition further showed that (13)CO2 assimilation by nitrifying communities depended solely on ammonia oxidation activity, suggesting a chemolithoautotrophic lifestyle. Phylogenetic analysis of both (13)C-labeled amoA and 16S rRNA genes revealed that most of the active AOA were phylogenetically closely related to the neutrophilic strains Nitrososphaera viennensis EN76 and JG1 within the Nitrososphaera cluster. Our results provide strong evidence for the adaptive growth of Nitrososphaera-like AOA in acidic soil, suggesting a greater metabolic versatility of soil AOA than previously appreciated.

Active Ammonia Oxidizers in an Acidic Soil Are Phylogenetically Closely Related to Neutrophilic Archaeon

PubMed Central

Wang, Baozhan; Zheng, Yan; Huang, Rong; Zhou, Xue; Wang, Dongmei; He, Yuanqiu

2014-01-01

All cultivated ammonia-oxidizing archaea (AOA) within the Nitrososphaera cluster (former soil group 1.1b) are neutrophilic. Molecular surveys also indicate the existence of Nitrososphaera-like phylotypes in acidic soil, but their ecological roles are poorly understood. In this study, we present molecular evidence for the chemolithoautotrophic growth of Nitrososphaera-like AOA in an acidic soil with pH 4.92 using DNA-based stable isotope probing (SIP). Soil microcosm incubations demonstrated that nitrification was stimulated by urea fertilization and accompanied by a significant increase in the abundance of AOA rather than ammonia-oxidizing bacteria (AOB). Real-time PCR analysis of amoA genes as a function of the buoyant density of the DNA gradient following the ultracentrifugation of the total DNA extracted from SIP microcosms indicated a substantial growth of soil AOA during nitrification. Pyrosequencing of the total 16S rRNA genes in the “heavy” DNA fractions suggested that archaeal communities were labeled to a much greater extent than soil AOB. Acetylene inhibition further showed that 13CO2 assimilation by nitrifying communities depended solely on ammonia oxidation activity, suggesting a chemolithoautotrophic lifestyle. Phylogenetic analysis of both 13C-labeled amoA and 16S rRNA genes revealed that most of the active AOA were phylogenetically closely related to the neutrophilic strains Nitrososphaera viennensis EN76 and JG1 within the Nitrososphaera cluster. Our results provide strong evidence for the adaptive growth of Nitrososphaera-like AOA in acidic soil, suggesting a greater metabolic versatility of soil AOA than previously appreciated. PMID:24375137

Response of bird species densities to habitat structure and fire history along a Midwestern open-forest gradient

USGS Publications Warehouse

Grundel, R.; Pavlovic, N.B.

2007-01-01

Oak savannas were historically common but are currently rare in the Midwestern United States. We assessed possible associations of bird species with savannas and other threatened habitats in the region by relating fire frequency and vegetation characteristics to seasonal densities of 72 bird species distributed across an open-forest gradient in northwestern Indiana. About one-third of the species did not exhibit statistically significant relationships with any combination of seven vegetation characteristics that included vegetation cover in five vertical strata, dead tree density, and tree height. For 40% of the remaining species, models best predicting species density incorporated tree density. Therefore, management based solely on manipulating tree density may not be an adequate strategy for managing bird populations along this open-forest gradient. Few species exhibited sharp peaks in predicted density under habitat conditions expected in restored savannas, suggesting that few savanna specialists occur among Midwestern bird species. When fire frequency, measured over fifteen years, was added to vegetation characteristics as a predictor of species density, it was incorporated into models for about one-quarter of species, suggesting that fire may modify habitat characteristics in ways that are important for birds but not captured by the structural habitat variables measured. Among those species, similar numbers had peaks in predicted density at low, intermediate, or high fire frequency. For species suggested by previous studies to have a preference for oak savannas along the open-forest gradient, estimated density was maximized at an average fire return interval of about one fire every three years. ?? The Cooper Ornithological Society 2007.

Is the bulk mode conversion important in high density helicon plasma?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Isayama, Shogo; Hada, Tohru; Shinohara, Shunjiro

2016-06-15

In a high-density helicon plasma production process, a contribution of Trivelpiece-Gould (TG) wave for surface power deposition is widely accepted. The TG wave can be excited either due to an abrupt density gradient near the plasma edge (surface conversion) or due to linear mode conversion from the helicon wave in a density gradient in the bulk region (bulk mode conversion). By numerically solving the boundary value problem of linear coupling between the helicon and the TG waves in a background with density gradient, we show that the efficiency of the bulk mode conversion strongly depends on the dissipation included inmore » the plasma, and the bulk mode conversion is important when the dissipation is small. Also, by performing FDTD simulation, we show the time evolution of energy flux associated with the helicon and the TG waves.« less

Hydrodynamic Model for Density Gradients Instability in Hall Plasmas Thrusters

NASA Astrophysics Data System (ADS)

Singh, Sukhmander

2017-10-01

There is an increasing interest for a correct understanding of purely growing electromagnetic and electrostatic instabilities driven by a plasma gradient in a Hall thruster devices. In Hall thrusters, which are typically operated with xenon, the thrust is provided by the acceleration of ions in the plasma generated in a discharge chamber. The goal of this paper is to study the instabilities due to gradients of plasma density and conditions for the growth rate and real part of the frequency for Hall thruster plasmas. Inhomogeneous plasmas prone a wide class of eigen modes induced by inhomogeneities of plasma density and called drift waves and instabilities. The growth rate of the instability has a dependences on the magnetic field, plasma density, ion temperature and wave numbers and initial drift velocities of the plasma species.

Gravity Gradient Tensor of Arbitrary 3D Polyhedral Bodies with up to Third-Order Polynomial Horizontal and Vertical Mass Contrasts

NASA Astrophysics Data System (ADS)

Ren, Zhengyong; Zhong, Yiyuan; Chen, Chaojian; Tang, Jingtian; Kalscheuer, Thomas; Maurer, Hansruedi; Li, Yang

2018-03-01

During the last 20 years, geophysicists have developed great interest in using gravity gradient tensor signals to study bodies of anomalous density in the Earth. Deriving exact solutions of the gravity gradient tensor signals has become a dominating task in exploration geophysics or geodetic fields. In this study, we developed a compact and simple framework to derive exact solutions of gravity gradient tensor measurements for polyhedral bodies, in which the density contrast is represented by a general polynomial function. The polynomial mass contrast can continuously vary in both horizontal and vertical directions. In our framework, the original three-dimensional volume integral of gravity gradient tensor signals is transformed into a set of one-dimensional line integrals along edges of the polyhedral body by sequentially invoking the volume and surface gradient (divergence) theorems. In terms of an orthogonal local coordinate system defined on these edges, exact solutions are derived for these line integrals. We successfully derived a set of unified exact solutions of gravity gradient tensors for constant, linear, quadratic and cubic polynomial orders. The exact solutions for constant and linear cases cover all previously published vertex-type exact solutions of the gravity gradient tensor for a polygonal body, though the associated algorithms may differ in numerical stability. In addition, to our best knowledge, it is the first time that exact solutions of gravity gradient tensor signals are derived for a polyhedral body with a polynomial mass contrast of order higher than one (that is quadratic and cubic orders). Three synthetic models (a prismatic body with depth-dependent density contrasts, an irregular polyhedron with linear density contrast and a tetrahedral body with horizontally and vertically varying density contrasts) are used to verify the correctness and the efficiency of our newly developed closed-form solutions. Excellent agreements are obtained between our solutions and other published exact solutions. In addition, stability tests are performed to demonstrate that our exact solutions can safely be used to detect shallow subsurface targets.

Density-Gradient Theory: A Macroscopic Approach to Quantum Confinement and Tunneling in Semiconductor Devices

DTIC Science & Technology

2011-01-01

that are attractive as luminescent biolabels, and possibly also for optoelectronic devices and solar cells . The equilibrium nature of such situations...The boundary layers as- sociated with the diffusion and Debye lengths are familiar, while that of LQ defines the layer in which the quantum in...circuits, transmission lines Diffusion -drift, density-gradient Semi-classical electron dynamics, Boltzmann transport Schrödinger, density- matrix, Wigner

DOE Office of Scientific and Technical Information (OSTI.GOV)

Constantin, Lucian A.; Fabiano, Eduardo; Della Sala, Fabio

We introduce a novel non-local ingredient for the construction of exchange density functionals: the reduced Hartree parameter, which is invariant under the uniform scaling of the density and represents the exact exchange enhancement factor for one- and two-electron systems. The reduced Hartree parameter is used together with the conventional meta-generalized gradient approximation (meta-GGA) semilocal ingredients (i.e., the electron density, its gradient, and the kinetic energy density) to construct a new generation exchange functional, termed u-meta-GGA. This u-meta-GGA functional is exact for the exchange of any one- and two-electron systems, is size-consistent and non-empirical, satisfies the uniform density scaling relation, andmore » recovers the modified gradient expansion derived from the semiclassical atom theory. For atoms, ions, jellium spheres, and molecules, it shows a good accuracy, being often better than meta-GGA exchange functionals. Our construction validates the use of the reduced Hartree ingredient in exchange-correlation functional development, opening the way to an additional rung in the Jacob’s ladder classification of non-empirical density functionals.« less

Multiphase Dynamics of Magma Oceans

NASA Astrophysics Data System (ADS)

Boukaré, Charles-Edouard; Ricard, Yanick; Parmentier, Edgar M.

2017-04-01

Since the earliest study of the Apollo lunar samples, the magma ocean hypothesis has received increasing consideration for explaining the early evolution of terrestrial planets. Giant impacts seem to be able to melt significantly large planets at the end of their accretion. The evolution of the resulting magma ocean would set the initial conditions (thermal and compositionnal structure) for subsequent long-term solid-state planet dynamics. However, magma ocean dynamics remains poorly understood. The major challenge relies on understanding interactions between the physical properties of materials (e.g., viscosity (at liquid or solid state), buoyancy) and the complex dynamics of an extremely vigorously convecting system. Such complexities might be neglected in cases where liquidus/adiabat interactions and density stratification leads to stable situations. However, interesting possibilities arise when exploring magma ocean dynamics in other regime. In the case of the Earth, recent studies have shown that the liquidus might intersect the adiabat at mid-mantle depth and/or that solids might be buoyant at deep mantle conditions. These results require the consideration of more sophisticated scenarios. For instance, how does bottom-up crystallization look with buoyant crystals? To understand this complex dynamics, we develop a multiphase phase numerical code that can handle simultaneously phase change, the convection in each phase and in the slurry, as well as the compaction or decompaction of the two phases. Although our code can only run in a limited parameter range (Rayleigh number, viscosity contrast between phases, Prandlt number), it provides a rich dynamics that illustrates what could have happened. For a given liquidus/adiabat configuration and density contrast between melt and solid, we explore magma ocean scenarios by varying the relative timescales of three first order processes: solid-liquid separation, thermo-chemical convective motions and magma ocean cooling.

Buoyant subduction on Venus: Implications for subduction around coronae

NASA Astrophysics Data System (ADS)

Burt, J. D.; Head, J. W.

1993-03-01

Potentially low lithospheric densities, caused by high Venus surface and perhaps mantle temperatures, could inhibit the development of negative buoyancy-driven subduction and a global system of plate tectonics/crustal recycling on that planet. No evidence for a global plate tectonic system was found so far, however, specific features strongly resembling terrestrial subduction zones in planform and topographic cross-section were described, including trenches around large coronae and chasmata in eastern Aphrodite Terra. The cause for the absence, or an altered expression, of plate tectonics on Venus remains to be found. Slab buoyancy may play a role in this difference, with higher lithospheric temperatures and a tendency toward positive buoyancy acting to oppose the descent of slabs and favoring under thrusting instead. The effect of slab buoyancy on subduction was explored and the conditions which would lead to under thrusting versus those allowing the formation of trenches and self-perpetuating subduction were defined. Applying a finite element code to assess the effects of buoyant forces on slabs subducting into a viscous mantle, it was found that mantle flow induced by horizontal motion of the convergent lithosphere greatly influences subduction angle, while buoyancy forces produce a lesser effect. Induced mantle flow tends to decrease subduction angle to near an under thrusting position when the subducting lithosphere converges on a stationary overriding lithosphere. When the overriding lithosphere is in motion, as in the case of an expanding corona, subduction angles are expected to increase. An initial stage involved estimating the changes in slab buoyancy due to slab healing and pressurization over the course of subduction. Modeling a slab, descending at a fixed angle and heated by conduction, radioactivity, and the heat released in phase changes, slab material density changes due to changing temperature, phase, and pressure were derived.

46 CFR 180.72 - Personal flotation devices carried in addition to life jackets.

Code of Federal Regulations, 2014 CFR

2014-10-01

... emergencies. (b) Wearable marine buoyant devices that include “ski vests,” “boating vests,” and “fishing vests... Commandant, may be carried as additional equipment. (c) Buoyant work vests approved in accordance with § 160...

46 CFR 180.72 - Personal flotation devices carried in addition to life jackets.

Code of Federal Regulations, 2013 CFR

2013-10-01

... emergencies. (b) Wearable marine buoyant devices that include “ski vests,” “boating vests,” and “fishing vests... Commandant, may be carried as additional equipment. (c) Buoyant work vests approved in accordance with § 160...

46 CFR 180.72 - Personal flotation devices carried in addition to life jackets.

Code of Federal Regulations, 2012 CFR

2012-10-01

... emergencies. (b) Wearable marine buoyant devices that include “ski vests,” “boating vests,” and “fishing vests... Commandant, may be carried as additional equipment. (c) Buoyant work vests approved in accordance with § 160...

77 FR 9859 - Lifesaving Equipment: Production Testing and Harmonization With International Standards

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-21

... inflatable buoyant apparatuses may be certified using either occupant weight standard. Likewise, equipment...-AB46 Lifesaving Equipment: Production Testing and Harmonization With International Standards AGENCY... lifesaving equipment to harmonize Coast Guard regulations for inflatable liferafts and inflatable buoyant...

46 CFR 115.808 - Lifesaving.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) Inspection of each life jacket, work vest, and marine buoyant device; (3) If used, inspection of the... of each inflatable liferaft, inflatable buoyant apparatus, and inflatable life jacket to determine... managing operator shall destroy, in the presence of the marine inspector, each life jacket, other personal...

Density measurements of the lithium fluoride/lithium sulfide eutectic at high temperature

NASA Astrophysics Data System (ADS)

Lloyd, Charles L.; Gilbert, James B.

1994-10-01

A straightforward and reliable method to determine densities of molten salts at high temperatures was de-veloped by Janz and Lorenz several years ago.[1] This method was followed in order to determine the density of the LiF/Li2S eutectic[2] over the temperature range of 1176 to 1355 K in which the eutectic is liquid. The rel-ative lack of data for this eutectic is surprising given its potential usefulness in the study of advanced batteries'31 and electrowinning of metals from molten sulfides.[41] The method is based on the fact that a solid piece of metal of known volume suspended from a pan balance into a molten salt will weigh less than if it were sus-pended in air at the same temperature. This difference in weight measured in grams will be equal to the buoyant force of the liquid at that temperature. The density of the salt bath can then readily be determined by dividing this difference by the volume of the solid piece of metal that is immersed in the bath. The procedure can be re-peated to give density values over a range of temperatures.

Isoelectric focusing of red blood cells in a density gradient stabilized column

NASA Technical Reports Server (NTRS)

Smolka, A. J. K.; Miller, T. Y.

1980-01-01

The effects of Ficoll and cell application pH on red blood cell electrophoretic mobility and focusing pH were investigated by focusing cells in a density gradient stabilized column. Sample loading, cell dispersion, column conductivity, resolution of separation, and the effect of Ampholines were examined.

Subduction of a buoyant plateau at the Manila Trench: Tomographic evidence and geodynamic implications

NASA Astrophysics Data System (ADS)

Fan, Jianke; Zhao, Dapeng; Dong, Dongdong

2016-02-01

We determined P-wave tomographic images by inverting a large number of arrival-time data from 2749 local earthquakes and 1462 teleseismic events, which are used to depict the three-dimensional morphology of the subducted Eurasian Plate along the northern segment of the Manila Trench. Dramatic changes in the dip angle of the subducted Eurasian Plate are revealed from the north to the south, being consistent with the partial subduction of a buoyant plateau beneath the Luzon Arc. Slab tears may exist along the edges of the buoyant plateau within the subducted plate induced by the plateau subduction, and the subducted lithosphere may be absent at depths greater than 250 km at ˜19°N and ˜21°N. The subducted buoyant plateau is possibly oriented toward NW-SE, and the subducted plate at ˜21°N is slightly steeper than that at ˜19°N. These results may explain why the western and eastern volcanic chains in the Luzon Arc are separated by ˜50 km at ˜18°N, whereas they converge into a single volcanic chain northward, which may be related to the oblique subduction along the Manila Trench caused by the northwestern movement of the Philippine Sea Plate. A low-velocity zone is revealed at depths of 20-200 km beneath the Manila Accretionary Prism at ˜22°N, suggesting that the subduction along the Manila Trench may stop there and the collision develops northward. The Taiwan Orogeny may originate directly from the subduction of the buoyant plateau, because the initial time of the Taiwan Orogeny is coincident with that of the buoyant plateau subduction.

Equatorial magnetic Rossby waves — evidence for a thin, strongly-buoyant stratified layer in earth's core

NASA Astrophysics Data System (ADS)

Knezek, Nicholas; Buffett, Bruce

2017-04-01

A low density stratified layer at the top of Earth's core has been proposed by many authors on the basis of chemical and thermodynamic arguments and has implications for Earth's thermal history, core energetics, and core-mantle interactions. Past studies claiming to detect a layer using perturbations in seismic wave speeds are contentious due to the extremely small magnitude of the detected signal. Recently, several studies have instead argued for the existence of a stratified layer by hypothesizing that oscillations in the observed geomagnetic field arise from waves propagating in the layer. In particular, 60 year oscillations in dipole strength have been attributed to global MAC waves, and 8 year oscillations of secular acceleration have been attributed to equatorially-trapped waves. We use a new hybrid finite-volume and Fourier numerical method we developed to model magnetohydrodynamic waves in a thin layer and show that a thin, strongly buoyant layer can produce equatorially-trapped waves with similar structures and periods to the observed 8 year signal. Using these simulated wave structures, we provide additional evidence for the existence of several propagating wave modes and place constraints on estimates for the wave periods, stratified layer thickness, and strength of buoyancy within the layer.

The foraging benefits of being fat in a highly migratory marine mammal

PubMed Central

Adachi, Taiki; Maresh, Jennifer L.; Robinson, Patrick W.; Peterson, Sarah H.; Costa, Daniel P.; Naito, Yasuhiko; Watanabe, Yuuki Y.; Takahashi, Akinori

2014-01-01

Foraging theory predicts that breath-hold divers adjust the time spent foraging at depth relative to the energetic cost of swimming, which varies with buoyancy (body density). However, the buoyancy of diving animals varies as a function of their body condition, and the effects of these changes on swimming costs and foraging behaviour have been poorly examined. A novel animal-borne accelerometer was developed that recorded the number of flipper strokes, which allowed us to monitor the number of strokes per metre swam (hereafter, referred to as strokes-per-metre) by female northern elephant seals over their months-long, oceanic foraging migrations. As negatively buoyant seals increased their fat stores and buoyancy, the strokes-per-metre increased slightly in the buoyancy-aided direction (descending), but decreased significantly in the buoyancy-hindered direction (ascending), with associated changes in swim speed and gliding duration. Overall, the round-trip strokes-per-metre decreased and reached a minimum value when seals achieved neutral buoyancy. Consistent with foraging theory, seals stayed longer at foraging depths when their round-trip strokes-per-metre was less. Therefore, neutrally buoyant divers gained an energetic advantage via reduced swimming costs, which resulted in an increase in time spent foraging at depth, suggesting a foraging benefit of being fat. PMID:25377461

In vitro production of beta-very low density lipoproteins and small, dense low density lipoproteins in mildly hypertriglyceridemic plasma: role of activities of lecithin:cholester acyltransferase, cholesterylester transfer proteins and lipoprotein lipase.

PubMed

Chung, B H; Segrest, J P; Franklin, F

1998-12-01

As a model for the formation of beta-very low density lipoproteins (VLDL) and small, dense LDL by the intraplasma metabolic activities in vivo, lipoproteins in fresh plasma were interacted in vitro with endogenous lecithin:cholesterol acyltransferase (LCAT) and cholesterylester transfer proteins (CETP) and subsequently with purified lipoprotein lipase (LpL). The LCAT and CETP reactions in a mildly hypertriglyceridemic (HTG) plasma at 37 degrees C for 18 h resulted in (1) esterification of about 45% plasma unesterified cholesterol (UC), (2) a marked increase in cholesterylester (CE) (+129%) and a decrease in triglyceride (TG) (-45%) in VLDL, and (3) a marked increase of TG (+ 341%) with a small net decrease of CE (-3.6%) in LDL, causing a significant alteration in the TG/CE of VLDL (from 8.0 to 1.9) and of LDL (from 0.20 to 0.93). The LDL in LCAT and CETP-reacted plasma is larger and more buoyant than that in control plasma. In vitro lipolysis of control and LCAT and CETP-reacted plasma by LpL, which hydrolyzed >90% of VLDL-TG and about 50-60% of LDL-TG, converted most of VLDL in control plasma (>85%) but less than half (40%) of VLDL in LCAT and CETP-reacted plasma into the IDL-LDL density fraction and transformed the large, buoyant LDL in the LCAT and CETP-reacted plasma into particles smaller and denser than those in the control plasma. The remnants that accumulated in the VLDL density region of the postlipolysis LCAT and CETP-reacted plasma contained apo B-100 and E but little or no detectable apo Cs and consisted of particles having pre-beta and beta-electrophoretic mobilities. The inhibition of LCAT during incubation of plasma, which lessened the extent of alteration in VLDL and LDL core lipids, increased the extent of lipolytic removal of VLDL from the VLDL density region but lowered the extent of alteration in the size and density of LDL. The LCAT, CETP and/or LpL-mediated alterations in the density of LDL in normolipidemic fasting plasma were less pronounced than that in mildly HTG plasma, but they became highly pronounced upon increase of its TG-rich lipoprotein level by the addition of preisolated VLDL or by the induction of postprandial lipemia. Although the effect of LCAT, CETP and LpL reactions in non-circulating plasma in vitro may be different from that in vivo, the above data suggests that the plasma TG-rich lipoprotein level and the extent of intraplasma LCAT, CETP, LpL and likely hepatic lipase (HL) reactions in vivo may play a role in determining the LDL phenotype.

Nonlinear Upshift of Trapped Electron Mode Critical Density Gradient: Simulation and Experiment

NASA Astrophysics Data System (ADS)

Ernst, D. R.

2012-10-01

A new nonlinear critical density gradient for pure trapped electron mode (TEM) turbulence increases strongly with collisionality, saturating at several times the linear threshold. The nonlinear TEM threshold appears to limit the density gradient in new experiments subjecting Alcator C-Mod internal transport barriers to modulated radio-frequency heating. Gyrokinetic simulations show the nonlinear upshift of the TEM critical density gradient is associated with long-lived zonal flow dominated states [1]. This introduces a strong temperature dependence that allows external RF heating to control TEM turbulent transport. During pulsed on-axis heating of ITB discharges, core electron temperature modulations of 50% were produced. Bursts of line-integrated density fluctuations, observed on phase contrast imaging, closely follow modulations of core electron temperature inside the ITB foot. Multiple edge fluctuation measurements show the edge response to modulated heating is out of phase with the core response. A new limit cycle stability diagram shows the density gradient appears to be clamped during on-axis heating by the nonlinear TEM critical density gradient, rather than by the much lower linear threshold. Fluctuation wavelength spectra will be quantitatively compared with nonlinear TRINITY/GS2 gyrokinetic transport simulations, using an improved synthetic diagnostic. In related work, we are implementing the first gyrokinetic exact linearized Fokker Planck collision operator [2]. Initial results show short wavelength TEMs are fully stabilized by finite-gyroradius collisional effects for realistic collisionalities. The nonlinear TEM threshold and its collisionality dependence may impact predictions of density peaking based on quasilinear theory, which excludes zonal flows.[4pt] In collaboration with M. Churchill, A. Dominguez, C. L. Fiore, Y. Podpaly, M. L. Reinke, J. Rice, J. L. Terry, N. Tsujii, M. A. Barnes, I. Bespamyatnov, R. Granetz, M. Greenwald, A. Hubbard, J. W. Hughes, M. Landreman, B. Li, Y. Ma, P. Phillips, M. Porkolab, W. Rowan, S. Wolfe, and S. Wukitch.[4pt] [1] D. R. Ernst et al., Proc. 21st IAEA Fusion Energy Conference, Chengdu, China, paper IAEA-CN-149/TH/1-3 (2006). http://www-pub.iaea.org/MTCD/Meetings/FEC200/th1-3.pdf[0pt] [2] B. Li and D.R. Ernst, Phys. Rev. Lett. 106, 195002 (2011).

Local relative density modulates failure and strength in vertically aligned carbon nanotubes.

PubMed

Pathak, Siddhartha; Mohan, Nisha; Decolvenaere, Elizabeth; Needleman, Alan; Bedewy, Mostafa; Hart, A John; Greer, Julia R

2013-10-22

Micromechanical experiments, image analysis, and theoretical modeling revealed that local failure events and compressive stresses of vertically aligned carbon nanotubes (VACNTs) were uniquely linked to relative density gradients. Edge detection analysis of systematically obtained scanning electron micrographs was used to quantify a microstructural figure-of-merit related to relative local density along VACNT heights. Sequential bottom-to-top buckling and hardening in stress-strain response were observed in samples with smaller relative density at the bottom. When density gradient was insubstantial or reversed, bottom regions always buckled last, and a flat stress plateau was obtained. These findings were consistent with predictions of a 2D material model based on a viscoplastic solid with plastic non-normality and a hardening-softening-hardening plastic flow relation. The hardening slope in compression generated by the model was directly related to the stiffness gradient along the sample height, and hence to the local relative density. These results demonstrate that a microstructural figure-of-merit, the effective relative density, can be used to quantify and predict the mechanical response.

3D Imaging of Density Gradients Using Plenoptic BOS

NASA Astrophysics Data System (ADS)

Klemkowsky, Jenna; Clifford, Chris; Fahringer, Timothy; Thurow, Brian

2016-11-01

The combination of background oriented schlieren (BOS) and a plenoptic camera, termed Plenoptic BOS, is explored through two proof-of-concept experiments. The motivation of this work is to provide a 3D technique capable of observing density disturbances. BOS uses the relationship between density and refractive index gradients to observe an apparent shift in a patterned background through image comparison. Conventional BOS systems acquire a single line-of-sight measurement, and require complex configurations to obtain 3D measurements, which are not always conducive to experimental facilities. Plenoptic BOS exploits the plenoptic camera's ability to generate multiple perspective views and refocused images from a single raw plenoptic image during post processing. Using such capabilities, with regards to BOS, provides multiple line-of-sight measurements of density disturbances, which can be collectively used to generate refocused BOS images. Such refocused images allow the position of density disturbances to be qualitatively and quantitatively determined. The image that provides the sharpest density gradient signature corresponds to a specific depth. These results offer motivation to advance Plenoptic BOS with an ultimate goal of reconstructing a 3D density field.

The impact of domain aspect ratio on the inverse cascade in rotationally constrained convection.

NASA Astrophysics Data System (ADS)

Julien, K. A.; Plumley, M.; Knobloch, E.

2017-12-01

Rotationally constrained convective flows are characterized as buoyantly unstable flows with a primary geostrophic balance (i.e. a pointwise balance between the Coriolis and pressure gradient forces). Such flows are known to occur within planetary and stellar interiors and also within isolated regions of the worlds oceans. Rapidly rotating Rayleigh-B'enard convection represents the simplest paradigm for investigations. Recent numerical studies, performed in square domains, have discovered the existence of a strong non-local inverse energy cascade that results in a box filling dipole vortex upon which geostrophic turbulent convection resides. Utilizing the non-hydrostatic quasi-geostrophic equations, the effect of domain aspect ratio on the inverse energy cascade is explored. As the domain aspect ratio becomes anisotropy it is demonstrated that the large-scale states evolve from vortical dipoles to jets. Properties of these jets will be presented and discussed.

The impact of domain aspect ratio on the inverse cascade in rotationally constrained convection

NASA Astrophysics Data System (ADS)

Julien, Keith; Knobloch, Edgar; Plumley, Meredith

2017-11-01

Rotationally constrained convective flows are characterized as buoyantly unstable flows with a primary geostrophic balance (i.e. a pointwise balance between the Coriolis and pressure gradient forces). Such flows are known to occur within planetary and stellar interiors and also within isolated regions of the worlds oceans. Rapidly rotating Rayleigh-Benard convection represents the simplest paradigm for investigations. Recent numerical studies, performed in square domains, have discovered the existence of a strong non-local inverse energy cascade that results in a box filling dipole vortex upon which geostrophic turbulent convection resides. Utilizing the non-hydrostatic quasi-geostrophic equations, the effect of domain aspect ratio on the inverse energy cascade is explored. As the domain aspect ratio becomes anisotropy it is demonstrated that the large-scale states evolve from vortical dipoles to jets. Properties of these jets will be presented and discussed.

Low-gravity fluid physics: A program overview

NASA Technical Reports Server (NTRS)

1990-01-01

An overview is presented of the microgravity fluid physics program at Lewis Research Center. One of the main reasons for conducting low gravity research in fluid physics is to study phenomena such as surface tension, interfacial contact angles, and diffusion independent of such gravitationally induced effects as buoyant convection. Fluid physics is at the heart of many space-based technologies including power systems, thermal control systems, and life support systems. Fundamental understanding of fluid physics is a key ingredient to successful space systems design. In addition to describing ground-based and space-based low-gravity facilities, selected experiments are presented which highlight Lewis work in fluid physics. These experiments can be categorized into five theme areas which summarize the work being conducted at Lewis for OSSA: (1) isothermal/iso-solutal capillary phenomena; (2) capillary phenomena with thermal/solutal gradients; (3) thermal-solutal convection; (4) first- and second-order phase transitions in a static fluid; and (5) multiphase flow.

46 CFR 160.052-8 - Marking.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-8 Marking. (a) Each buoyant vest must have the following information clearly... vests shall be sufficiently waterproof so that after 72 hours submergence in water it will withstand...

46 CFR 160.052-8 - Marking.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-8 Marking. (a) Each buoyant vest must have the following information clearly... vests shall be sufficiently waterproof so that after 72 hours submergence in water it will withstand...

46 CFR 160.052-8 - Marking.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam, Adult and Child § 160.052-8 Marking. (a) Each buoyant vest must have the following information clearly... vests shall be sufficiently waterproof so that after 72 hours submergence in water it will withstand...

Automated Processing of Plasma Samples for Lipoprotein Separation by Rate-Zonal Ultracentrifugation.

PubMed

Peters, Carl N; Evans, Iain E J

2016-12-01

Plasma lipoproteins are the primary means of lipid transport among tissues. Defining alterations in lipid metabolism is critical to our understanding of disease processes. However, lipoprotein measurement is limited to specialized centers. Preparation for ultracentrifugation involves the formation of complex density gradients that is both laborious and subject to handling errors. We created a fully automated device capable of forming the required gradient. The design has been made freely available for download by the authors. It is inexpensive relative to commercial density gradient formers, which generally create linear gradients unsuitable for rate-zonal ultracentrifugation. The design can easily be modified to suit user requirements and any potential future improvements. Evaluation of the device showed reliable peristaltic pump accuracy and precision for fluid delivery. We also demonstrate accurate fluid layering with reduced mixing at the gradient layers when compared to usual practice by experienced laboratory personnel. Reduction in layer mixing is of critical importance, as it is crucial for reliable lipoprotein separation. The automated device significantly reduces laboratory staff input and reduces the likelihood of error. Overall, this device creates a simple and effective solution to formation of complex density gradients. © 2015 Society for Laboratory Automation and Screening.

DNA stable-isotope probing (DNA-SIP).

PubMed

Dunford, Eric A; Neufeld, Josh D

2010-08-02

DNA stable-isotope probing (DNA-SIP) is a powerful technique for identifying active microorganisms that assimilate particular carbon substrates and nutrients into cellular biomass. As such, this cultivation-independent technique has been an important methodology for assigning metabolic function to the diverse communities inhabiting a wide range of terrestrial and aquatic environments. Following the incubation of an environmental sample with stable-isotope labelled compounds, extracted nucleic acid is subjected to density gradient ultracentrifugation and subsequent gradient fractionation to separate nucleic acids of differing densities. Purification of DNA from cesium chloride retrieves labelled and unlabelled DNA for subsequent molecular characterization (e.g. fingerprinting, microarrays, clone libraries, metagenomics). This JoVE video protocol provides visual step-by-step explanations of the protocol for density gradient ultracentrifugation, gradient fractionation and recovery of labelled DNA. The protocol also includes sample SIP data and highlights important tips and cautions that must be considered to ensure a successful DNA-SIP analysis.

Chemical trends in ocean islands explained by plume–slab interaction

NASA Astrophysics Data System (ADS)

Dannberg, Juliane; Gassmöller, Rene

2018-04-01

Earth's surface shows many features, of which the genesis can be understood only through their connection with processes in Earth's deep interior. Recent studies indicate that spatial geochemical patterns at oceanic islands correspond to structures in the lowermost mantle inferred from seismic tomographic models. This suggests that hot, buoyant upwellings can carry chemical heterogeneities from the deep lower mantle toward the surface, providing a window to the composition of the lowermost mantle. The exact nature of this link between surface and deep Earth remains debated and poorly understood. Using computational models, we show that subducted slabs interacting with dense thermochemical piles can trigger the ascent of hot plumes that inherit chemical gradients present in the lowermost mantle. We identify two key factors controlling this process: (i) If slabs induce strong lower-mantle flow toward the edges of these piles where plumes rise, the pile-facing side of the plume preferentially samples material originating from the pile, and bilaterally asymmetric chemical zoning develops. (ii) The composition of the melt produced reflects this bilateral zoning if the overlying plate moves roughly perpendicular to the chemical gradient in the plume conduit. Our results explain some of the observed geochemical trends of oceanic islands and provide insights into how these trends may originate.

Preliminary study of ground handling characteristics of Buoyant Quad Rotor (BQR) vehicles

NASA Technical Reports Server (NTRS)

Browning, R. G. E.

1980-01-01

A preliminary investigation of mooring concepts appropriate for heavy lift buoyant quad rotor (BQR) vehicles was performed. A review of the evolution of ground handling systems and procedures for all airship types is presented to ensure that appropriate consideration is given to past experiences. Two buoyant quad rotor designs are identified and described. An analysis of wind loads on a moored airship and the effects of these loads on vehicle design is provided. Four mooring concepts are assessed with respect to the airship design, wind loads and mooring site considerations. Basing requirements and applicability of expeditionary mooring at various operational scenarios are addressed.

Small explosive volcanic plume dynamics: insights from feature tracking velocimetry at Santiaguito lava dome

NASA Astrophysics Data System (ADS)

Benage, M. C.; Andrews, B. J.

2016-12-01

Volcanic explosions eject turbulent, transient jets of hot volcanic gas and particles into the atmosphere. Though the jet of hot material is initially negatively buoyant, the jet can become buoyant through entrainment and subsequent thermal expansion of entrained air that allows the eruptive plume to rise several kilometers. Although basic plume structure is qualitatively well known, the velocity field and dynamic structure of volcanic plumes are not well quantified. An accurate and quantitative description of volcanic plumes is essential for hazard assessments, such as if the eruption will form a buoyant plume that will affect aviation or produce dangerous pyroclastic density currents. Santa Maria volcano, in Guatemala, provides the rare opportunity to safely capture video of Santiaguito lava dome explosions and small eruptive plumes. In January 2016, two small explosions (< 2 km) that lasted several minutes and with little cloud obstruction were recorded for image analysis. The volcanic plume structure is analyzed through sequential image frames from the video where specific features are tracked using a feature tracking velocimetry (FTV) algorithm. The FTV algorithm quantifies the 2D apparent velocity fields along the surface of the plume throughout the duration of the explosion. Image analysis of small volcanic explosions allows us to examine the maximum apparent velocities at two heights above the dome surface, 0-25 meters, where the explosions first appear, and 100-125 meters. Explosions begin with maximum apparent velocities of <15 m/s. We find at heights near the dome surface and 10 seconds after explosion initiation, the maximum apparent velocities transition to sustained velocities of 5-15 m/s. At heights 100-125 meters above the dome surface, the apparent velocities transition to sustained velocities of 5-15 m/s after 25 seconds. Throughout the explosion, transient velocity maximums can exceed 40 m/s at both heights. Here, we provide novel quantification and description of turbulent surface velocity fields of explosive volcanic eruptions at active lava domes.

46 CFR 160.049-1 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Cushion Plastic Foam § 160.049-1... Guard specifications: 160.055—Life Preservers, Unicellular Plastic Foam, Adult and Child. 164.015—Plastic Foam, Unicellular, Buoyant, Sheet and Molded Shapes. (4) Military specifications. MIL-C-43006...

46 CFR 160.049-1 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Cushion Plastic Foam § 160.049-1... Guard specifications: 160.055—Life Preservers, Unicellular Plastic Foam, Adult and Child. 164.015—Plastic Foam, Unicellular, Buoyant, Sheet and Molded Shapes. (4) Military specifications. MIL-C-43006...

46 CFR 160.047-3 - Materials.

Code of Federal Regulations, 2010 CFR

2010-10-01

... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS... and Child § 160.047-3 Materials. (a) General. All components used in the construction of buoyant vests... loops for an adult or child size buoyant vest specified by this subpart must be 3/4-inch cotton webbing...

Plasma Irregularities on the Leading and Trailing Edges of Polar Cap Patches

NASA Astrophysics Data System (ADS)

Lamarche, L. J.; Varney, R. H.; Gillies, R.; Chartier, A.; Mitchell, C. N.

2017-12-01

Plasma irregularities in the polar cap have often been attributed to the gradient drift instability (GDI). Traditional fluid theories of GDI predicts irregularity growth only on the trailing edge of polar patches, where the plasma density gradient is parallel to the plasma drift velocity, however many observations show irregularities also form on the leading edge of patches. We consider decameter-scale irregularities detected by polar-latitude SuperDARN (Super Dual Auroral Radar Network) radars with any relationship between the background density gradients and drift velocity. Global electron density from the Multi-Instrument Data Analysis System (MIDAS), a GPS tomography routine, is used to provide context for where irregularities are observed relative to polar patches and finer-scale background density gradients are found from 3D imaging from both the North and Canada faces of the Resolute Bay Incoherent Scatter Radars (RISR-N and RISR-C) jointly. Shear-based instabilities are considered as mechanisms by which plasma irregularities could form on the leading edge of patches. Theoretical predictions of instability growth from both GDI and shear instabilities are compared with irregularity observations for the October 13, 2016 storm.

Effect of Water Surface Salinity on Evaporation: The Case of a Diluted Buoyant Plume Over the Dead Sea

NASA Astrophysics Data System (ADS)

Mor, Z.; Assouline, S.; Tanny, J.; Lensky, I. M.; Lensky, N. G.

2018-03-01

Evaporation from water bodies strongly depends on surface water salinity. Spatial variation of surface salinity of saline water bodies commonly occurs across diluted buoyant plumes fed by freshwater inflows. Although mainly studied at the pan evaporation scale, the effect of surface water salinity on evaporation has not yet been investigated by means of direct measurement at the scale of natural water bodies. The Dead Sea, a large hypersaline lake, is fed by onshore freshwater springs that form local diluted buoyant plumes, offering a unique opportunity to explore this effect. Surface heat fluxes, micrometeorological variables, and water temperature and salinity profiles were measured simultaneously and directly over the salty lake and over a region of diluted buoyant plume. Relatively close meteorological conditions prevailed in the two regions; however, surface water salinity was significantly different. Evaporation rate from the diluted plume was occasionally 3 times larger than that of the main salty lake. In the open lake, where salinity was uniform with depth, increased wind speed resulted in increased evaporation rate, as expected. However, in the buoyant plume where diluted brine floats over the hypersaline brine, wind speed above a threshold value (˜4 m s-1) caused a sharp decrease in evaporation probably due to mixing of the stratified plume and a consequent increase in the surface water salinity.

Buoyancy instability of homologous implosions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, B. M.

2015-06-15

With this study, I consider the hydrodynamic stability of imploding ideal gases as an idealized model for inertial confinement fusion capsules, sonoluminescent bubbles and the gravitational collapse of astrophysical gases. For oblate modes (short-wavelength incompressive modes elongated in the direction of the mean flow), a second-order ordinary differential equation is derived that can be used to assess the stability of any time-dependent flow with planar, cylindrical or spherical symmetry. Upon further restricting the analysis to homologous flows, it is shown that a monatomic gas is governed by the Schwarzschild criterion for buoyant stability. Under buoyantly unstable conditions, both entropy andmore » vorticity fluctuations experience power-law growth in time, with a growth rate that depends upon mean flow gradients and, in the absence of dissipative effects, is independent of mode number. If the flow accelerates throughout the implosion, oblate modes amplify by a factor (2C) |N0|ti, where C is the convergence ratio of the implosion, N 0 is the initial buoyancy frequency and t i is the implosion time scale. If, instead, the implosion consists of a coasting phase followed by stagnation, oblate modes amplify by a factor exp(π|N 0|t s), where N 0 is the buoyancy frequency at stagnation and t s is the stagnation time scale. Even under stable conditions, vorticity fluctuations grow due to the conservation of angular momentum as the gas is compressed. For non-monatomic gases, this additional growth due to compression results in weak oscillatory growth under conditions that would otherwise be buoyantly stable; this over-stability is consistent with the conservation of wave action in the fluid frame. The above analytical results are verified by evolving the complete set of linear equations as an initial value problem, and it is demonstrated that oblate modes are the fastest-growing modes and that high mode numbers are required to reach this limit (Legendre mode ℓ ≳ 100 for spherical flows). Finally, comparisons are made with a Lagrangian hydrodynamics code, and it is found that a numerical resolution of ~30 zones per wavelength is required to capture these solutions accurately. This translates to an angular resolution of ~(12/ℓ)°, or ≲ 0.1° to resolve the fastest-growing modes.« less

Density gradient in SiO 2 films on silicon as revealed by positron annihilation spectroscopy

NASA Astrophysics Data System (ADS)

Revesz, A. G.; Anwand, W.; Brauer, G.; Hughes, H. L.; Skorupa, W.

2002-06-01

Positron annihilation spectroscopy of thermally grown and deposited SiO 2 films on silicon shows in a non-destructive manner that these films have a gradient in their density. The gradient is most pronounced for the oxide grown in dry oxygen. Oxidation in water-containing ambient results in an oxide with reduced gradient, similarly to the gradient in the deposited oxide. These observations are in accordance with earlier optical and other studies using stepwise etching or a set of samples of varying thickness. The effective oxygen charge, which is very likely one of the reasons for the difference in the W parameters of silica glass and quartz crystal, could be even higher at some localized configurations in the SiO 2 films resulting in increased positron trapping.

Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa.

PubMed

Nicolas, M; Alvarez, M; Borragán, S; Martinez-Pastor, F; Chamorro, C A; Alvarez-Rodriguez, M; de Paz, P; Anel, L

2012-04-01

Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample. Copyright © 2012 Elsevier Inc. All rights reserved.

Effects of semen storage and separation techniques on sperm DNA fragmentation.

PubMed

Jackson, Robert E; Bormann, Charles L; Hassun, Pericles A; Rocha, André M; Motta, Eduardo L A; Serafini, Paulo C; Smith, Gary D

2010-12-01

To determine the effect of semen storage and separation techniques on sperm DNA fragmentation. Controlled clinical study. An assisted reproductive technology laboratory. Thirty normoozospermic semen samples obtained from patients undergoing infertility evaluation. One aliquot from each sample was immediately prepared (control) for the sperm chromatin dispersion assay (SCD). Aliquots used to assess storage techniques were treated in the following ways: snap frozen by liquid nitrogen immersion, slow frozen with Tris-yolk buffer and glycerol, kept on ice for 24 hours or maintained at room temperature for 4 and 24 hours. Aliquots used to assess separation techniques were processed by the following methods: washed and centrifuged in media, swim-up from washed sperm pellet, density gradient separation, density gradient followed by swim-up. DNA integrity was then measured by SCD. DNA fragmentation as measured by SCD. There was no significant difference in fragmentation among the snap frozen, slow frozen, and wet-ice groups. Compared to other storage methods short-term storage at room temperature did not impact DNA fragmentation yet 24 hours storage significantly increased fragmentation. Swim-up, density gradient and density gradient/swim-up had significantly reduced DNA fragmentation levels compared with washed semen. Postincubation, density gradient/swim-up showed the lowest fragmentation levels. The effect of sperm processing methods on DNA fragmentation should be considered when selecting storage or separation techniques for clinical use. Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

The hard parts (trophi) of the rotifer mastax do contain chitin: evidence from studies on Brachionus plicatilis.

PubMed

Klusemann, J; Kleinow, W; Peters, W

1990-01-01

The jaws (trophi) of the rotifer Brachionus plicatilis are soluble in strong acids but are resistant to long treatments by strong alkali. They show the same buoyant density as chitin and also as the chitin-containing layers of rotifer egg-shells. The presence of chitin in these structures was confirmed using the following techniques: chitosan-tests, thin-layer chromatography of trophi-hydrolysates which revealed glucosamine, by dissolving trophi with chitinase and electron microscopic WGA/gold-labelling. The content of chitin in the trophi was estimated by two different methods to be approx. 64% (50-75%).

Amphibian egg cytoplasm response to altered g-forces and gravity orientation

NASA Technical Reports Server (NTRS)

Neff, A. W.; Smith, R. C.; Malacinski, G. M.

1986-01-01

Elucidation of dorsal/ventral polarity and primary embryonic axis development in amphibian embryos requires an understanding of cytoplasmic rearrangements in fertile eggs at the biophysical, physiological, and biochemical levels. Evidence is presented that amphibian egg cytoplasmic components are compartmentalized. The effects of altered orientation to the gravitational vector (i.e., egg inversion) and alterations in gravity force ranging from hypergravity (centrifugation) to simulated microgravity (i.e., horizontal clinostat rotation) on cytoplasmic compartment rearrangements are reviewed. The behavior of yolk compartments as well as a newly defined (with monoclonal antibody) nonyolk cytoplasmic compartment, in inverted eggs and in eggs rotated on horizontal clinostats at their buoyant density, is discussed.

Buoyancy effects on smoldering combustion

NASA Technical Reports Server (NTRS)

Dosanjh, S.; Peterson, J.; Fernandez-Pello, A. C.; Pagni, P. J.

1985-01-01

The effect of buoyancy on the rate of spread of a concurrent smolder reaction through a porous combustible material is investigated theoretically and experimentally. In the experiments, buoyant forces are controlled by varying the density difference, and the smolder rate spread through porous alpha cellulose (0.83 void fraction) is measured as a function of the ambient air pressure. The smolder velocity is found to increase with the ambient pressure; extinction occurs when the buoyancy forces cannot overcome the drag forces, indicating that diffusion by itself cannot support the spread of a smolder reaction. Theoretical predictions are found to be in good qualitative agreement with the experimental results.

On the Internal Structure of Bacteriophage Lambda

PubMed Central

Kaiser, A. D.

1966-01-01

The structure of bacteriophage lambda has been studied by electron microscopy of negatively stained particles. The phage particles will eject their DNA if they are heated or dialyzed against a chelating agent. The ghost particles, so formed, have a channel running down their tails. Since the channel is not visible in normal particles, the channel may be filled with part of the DNA molecule. Up to 30% of the ghosts contain round objects about half the internal diameter of the head. The round objects, called "cores," have the same buoyant density as the coat protein. The core may be a protein spool about which the phage DNA is wound. PMID:5967429

Evidence of toroidally localized turbulence with applied 3D fields in the DIII-D tokamak

DOE PAGES

Wilcox, R. S.; Shafer, M. W.; Ferraro, N. M.; ...

2016-09-21

New evidence indicates that there is significant 3D variation in density fluctuations near the boundary of weakly 3D tokamak plasmas when resonant magnetic perturbations are applied to suppress transient edge instabilities. The increase in fluctuations is concomitant with an increase in the measured density gradient, suggesting that this toroidally localized gradient increase could be a mechanism for turbulence destabilization in localized flux tubes. Two-fluid magnetohydrodynamic simulations find that, although changes to the magnetic field topology are small, there is a significant 3D variation of the density gradient within the flux surfaces that is extended along field lines. This modeling agreesmore » qualitatively with the measurements. The observed gradient and fluctuation asymmetries are proposed as a mechanism by which global profile gradients in the pedestal could be relaxed due to a local change in the 3D equilibrium. In conclusion, these processes may play an important role in pedestal and scrape-off layer transport in ITER and other future tokamak devices with small applied 3D fields.« less

Method and apparatus for ceramic analysis

DOEpatents

Jankowiak, Ryszard J.; Schilling, Chris; Small, Gerald J.; Tomasik, Piotr

2003-04-01

The present invention relates to a method and apparatus for ceramic analysis, in particular, a method for analyzing density, density gradients and/or microcracks, including an apparatus with optical instrumentation for analysis of density, density gradients and/or microcracks in ceramics. The method provides analyzing density of a ceramic comprising exciting a component on a surface/subsurface of the ceramic by exposing the material to excitation energy. The method may further include the step of obtaining a measurement of an emitted energy from the component. The method may additionally include comparing the measurement of the emitted energy from the component with a predetermined reference measurement so as to obtain a density for said ceramic.

A Theory of Density Layering in Stratified Turbulence using Statistical State Dynamics

NASA Astrophysics Data System (ADS)

Fitzgerald, J.; Farrell, B.

2016-12-01

Stably stratified turbulent fluids commonly develop density structures that are layered in the vertical direction (e.g., Manucharyan et al., 2015). Within layers, density is approximately constant and stratification is weak. Between layers, density varies rapidly and stratification is strong. A common explanation for the existence of layers invokes the negative diffusion mechanism of Phillips (1972) & Posmentier (1977). The physical principle underlying this mechanism is that the flux-gradient relationship connecting the turbulent fluxes of buoyancy to the background stratification must have the special property of weakening fluxes with strengthening gradient. Under these conditions, the evolution of the stratification is governed by a negative diffusion problem which gives rise to spontaneous layer formation. In previous work on stratified layering, this flux-gradient property is often assumed (e.g, Posmentier, 1977) or drawn from phenomenological models of turbulence (e.g., Balmforth et al., 1998).In this work we develop the theoretical underpinnings of layer formation by applying stochastic turbulence modeling and statistical state dynamics (SSD) to predict the flux-gradient relation and analyze layer formation directly from the equations of motion. We show that for stochastically-forced homogeneous 2D Boussinesq turbulence, the flux-gradient relation can be obtained analytically and indicates that the fluxes always strengthen with stratification. The Phillips mechanism thus does not operate in this maximally simplified scenario. However, when the problem is augmented to include a large scale background shear, we show that the flux-gradient relationship is modified so that the fluxes weaken with stratification. Sheared and stratified 2D Boussinesq turbulence thus spontaneously forms density layers through the Phillips mechanism. Using SSD (Farrell & Ioannou 2003), we obtain a closed, deterministic dynamics for the stratification and the statistical turbulent state. We show that density layers form as a linear instability of the sheared turbulence, associated with a supercritical bifurcation. We further show that SSD predicts the nonlinear equilibration and maintenance of the layers, and captures the phenomena of layer growth and mergers (Radko, 2007).

46 CFR 160.048-1 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Cushion, Fibrous Glass § 160.048-1... documents: (1) Military specification: MIL-B-2766—Batt, Fibrous Glass, Lifesaving Equipment. (2) Federal... issue in effect on the date kapok or fibrous glass buoyant cushions are manufactured, form a part of...

46 CFR 160.052-1 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam... Preservers, Unicellular Plastic Foam, Adult and Child. 164.015—Plastic Foam, Unicellular, Buoyant Sheet and... manufactured, form a part of this subpart: Dwg. No. 160.052-1: Sheet 1—Cutting Pattern and General Arrangement...

46 CFR 160.052-1 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam... Preservers, Unicellular Plastic Foam, Adult and Child. 164.015—Plastic Foam, Unicellular, Buoyant Sheet and... manufactured, form a part of this subpart: Dwg. No. 160.052-1: Sheet 1—Cutting Pattern and General Arrangement...

46 CFR 160.052-1 - Incorporation by reference.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam... Preservers, Unicellular Plastic Foam, Adult and Child. 164.015—Plastic Foam, Unicellular, Buoyant Sheet and... manufactured, form a part of this subpart: Dwg. No. 160.052-1: Sheet 1—Cutting Pattern and General Arrangement...

46 CFR 160.052-1 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Plastic Foam... Preservers, Unicellular Plastic Foam, Adult and Child. 164.015—Plastic Foam, Unicellular, Buoyant Sheet and... manufactured, form a part of this subpart: Dwg. No. 160.052-1: Sheet 1—Cutting Pattern and General Arrangement...

46 CFR 169.556 - Work vests.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Work vests. 169.556 Section 169.556 Shipping COAST GUARD... Firefighting Equipment Additional Lifesaving Equipment § 169.556 Work vests. (a) Buoyant work vests carried.... (b) Approved buoyant work vests are items of safety apparel and may be carried aboard vessels to be...

46 CFR 169.556 - Work vests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Work vests. 169.556 Section 169.556 Shipping COAST GUARD... Firefighting Equipment Additional Lifesaving Equipment § 169.556 Work vests. (a) Buoyant work vests carried.... (b) Approved buoyant work vests are items of safety apparel and may be carried aboard vessels to be...

46 CFR 160.073-1 - Scope.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Float-Free Link or Life Floats and Buoyant Apparatus § 160.073-1 Scope. (a... apparatus painter to a vessel. The float-free link is designed to be broken by the buoyant force of the life...

46 CFR 160.060-2 - Type and model.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-2 Type and model. Each buoyant vest specified in this subpart is a: (a... designation for a nonstandard vest is to be assigned by the individual manufactured and must be different from...

46 CFR 169.556 - Work vests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Work vests. 169.556 Section 169.556 Shipping COAST GUARD... Firefighting Equipment Additional Lifesaving Equipment § 169.556 Work vests. (a) Buoyant work vests carried.... (b) Approved buoyant work vests are items of safety apparel and may be carried aboard vessels to be...

46 CFR 160.060-2 - Type and model.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-2 Type and model. Each buoyant vest specified in this subpart is a: (a... designation for a nonstandard vest is to be assigned by the individual manufactured and must be different from...

46 CFR 160.060-2 - Type and model.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-2 Type and model. Each buoyant vest specified in this subpart is a: (a... designation for a nonstandard vest is to be assigned by the individual manufactured and must be different from...

46 CFR 169.556 - Work vests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Work vests. 169.556 Section 169.556 Shipping COAST GUARD... Firefighting Equipment Additional Lifesaving Equipment § 169.556 Work vests. (a) Buoyant work vests carried.... (b) Approved buoyant work vests are items of safety apparel and may be carried aboard vessels to be...

46 CFR 160.060-2 - Type and model.

Code of Federal Regulations, 2011 CFR

2011-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160.060-2 Type and model. Each buoyant vest specified in this subpart is a: (a... designation for a nonstandard vest is to be assigned by the individual manufactured and must be different from...

46 CFR 169.556 - Work vests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Work vests. 169.556 Section 169.556 Shipping COAST GUARD... Firefighting Equipment Additional Lifesaving Equipment § 169.556 Work vests. (a) Buoyant work vests carried.... (b) Approved buoyant work vests are items of safety apparel and may be carried aboard vessels to be...

29 CFR 1926.106 - Working over or near water.

Code of Federal Regulations, 2010 CFR

2010-07-01

... drowning exists, shall be provided with U.S. Coast Guard-approved life jacket or buoyant work vests. (b) Prior to and after each use, the buoyant work vests or life preservers shall be inspected for defects... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Personal Protective and Life Saving Equipment...

Plasma Irregularity Production in the Polar Cap F-Region Ionosphere

NASA Astrophysics Data System (ADS)

Lamarche, Leslie

Plasma in the Earth's ionosphere is highly irregular on scales ranging between a few centimeters and hundreds of kilometers. Small-scale irregularities or plasma waves can scatter radio waves resulting in a loss of signal for navigation and communication networks. The polar region is particularly susceptible to strong disturbances due to its direct connection with the Sun's magnetic field and energetic particles. In this thesis, factors that contribute to the production of decameter-scale plasma irregularities in the polar F region ionosphere are investigated. Both global and local control of irregularity production are studied, i.e. we consider global solar control through solar illumination and solar wind as well as much more local control by plasma density gradients and convection electric field. In the first experimental study, solar control of irregularity production is investigated using the Super Dual Auroral Radar Network (SuperDARN) radar at McMurdo, Antarctica. The occurrence trends for irregularities are analyzed statistically and a model is developed that describes the location of radar echoes within the radar's field-of-view. The trends are explained through variations in background plasma density with solar illumination affecting radar beam propagation. However, it is found that the irregularity occurrence during the night is higher than expected from ray tracing simulations based on a standard ionospheric density model. The high occurrence at night implies an additional source of plasma density and it is proposed that large-scale density enhancements called polar patches may be the source of this density. Additionally, occurrence maximizes around the terminator due to different competing irregularity production processes that favor a more or less sunlit ionosphere. The second study is concerned with modeling irregularity characteristics near a large-scale density gradient reversal, such as those expected near polar patches, with a particular focus on the asymmetry of the irregularity growth rate across the gradient reversal. Directional dependencies on the plasma density gradient, plasma drift, and wavevector are analyzed in the context of the recently developed general fluid theory of the gradient-drift instability. In the ionospheric F region, the strongest asymmetry is found when an elongated structure is oriented along the radar's boresight and moving perpendicular to its direction of elongation. These results have important implications for finding optimal configurations for oblique-scanning ionospheric radars such as SuperDARN to observe gradient reversals. To test the predictions of the developed model and the general theory of the gradient-drift instability, an experimental investigation is presented focusing on decameter-scale irregularities near a polar patch and the previously uninvestigated directional dependence of irregularity characteristics. Backscatter power and occurrence of irregularities are analyzed using measurements from the SuperDARN radar at Rankin Inlet, Canada, while background density gradients and convection electric fields are found from the north face of the Resolute Bay Incoherent Scatter Radar. It is shown that irregularity occurrence tends to follow the expected trends better than irregularity power, suggesting that while the gradient-drift instability may be a dominant process in generating small-scale irregularities, other mechanisms such as a shear-driven instability or nonlinear process may exert greater control over their intensity. It is concluded from this body of work that the production of small-scale plasma irregularities in the polar F-region ionosphere is controlled both by global factors such as solar illumination as well as local plasma density gradients and electric fields. In general, linear gradient-drift instability theory describes small-scale irregularity production well, particularly for low-amplitude perturbations. The production of irregularities is complex, and while ground-based radars are invaluable tools to study the ionosphere, care must be taken to interpret results correctly.

Origin of Stability in Particle Sedimentation

NASA Technical Reports Server (NTRS)

Segre, Philip N.

2003-01-01

Particle Image Velocimetry (PIV) is used to study the slow settling motions of spheres in suspensions ranging from dilute to highly concentrated, 0.0001 less than phi less than 0.50. During sedimentation, particle velocity fluctuations are found to be organized into regions of characteristic size xi approximately 11 a phi (exp -1/3). A simple model, based upon buoyant mass fluctuations DELTAm given by random density fluctuations in a region of size xi, accurately predicts the magnitudes of the velocity fluctuations DELTAV. We also find a new universal relation for particle diffusion during sedimentation. It can be written in a Stokes-Einstein form as Dapproximately(DELTAmxi)/(6pietaxi), where the effective temperature DELTAmgxi is the gravitational potential energy of density fluctuations. In addition related experiments examining inertial effects and transient states, that are aimed at uncovering the origin of the new lengthscale xi, will also be given.

Impacts of blending on dilution of negatively buoyant brine discharge in a shallow tidal sea.

PubMed

Kämpf, Jochen

2009-07-01

A fine-resolution three-dimensional hydrodynamic model is applied to study the dilution of desalination brine discharged into a tidal sea. Based on given inflow rate and salinity excess of discharge brine, this study explores variations in mid-field dilutions when other low-salinity wastewater is added to the discharge. Findings reveal that this blending leads to a decrease in dilution in the mixing zone and therefore to higher levels of pollutants in this zone, while, on the other hand, the mixing zone occupies a smaller area. The reason is that the discharge of brine creates a density-driven flow that operates to partially remove effluent from the discharge location. This removal is less efficient for the decrease in density excess of the discharge. Hence, in an ambient sea of moderate mixing, blending can be expected to increase the risk of marine pollution in the mixing zone.

Role of turbulence regime on determining the local density gradient

DOE PAGES

Wang, X.; Mordijck, Saskia; Doyle, E. J.; ...

2017-11-16

In this study we show that the local density gradient in the plasma core depends on the calculated mode-frequency of the most unstable linear mode and reaches a maximum when this frequency is close to zero. Previous theoretical and experimental work on AUG has shown that the ratio of electron to ion temperature, and as such the frequency of the dominant linear gyrokinetic mode, affects the local density gradient close to ρ = 0.3 [1, 2]. On DIII-D we find that by adding Electron Cyclotron Heating (ECH), we modify the dominant unstable linear gyro kinetic mode from an Ion Temperaturemore » Gradient (ITG) mode to a Trapped Electron Mode (TEM), which means that the frequency of the dominant mode changes sign (from the ion to the electron direction). Local density peaking around mid-radius increases by 50% right around the cross-over between the ITG and TEM regimes. By comparing how the particle flux changes, through the derivative of the electron density, n e, with respect to time, ∂n e/∂t, we find that the particle flux also exhibits the same trend versus mode frequency. As a result, we find that the changes in local particle transport are inversely proportional to the changes in electron density, indicating that the changes are driven by a change in thermo-diffusive pinch.« less

Double-hybrid density-functional theory with meta-generalized-gradient approximations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Souvi, Sidi M. O., E-mail: sidi.souvi@irsn.fr; Sharkas, Kamal; Toulouse, Julien, E-mail: julien.toulouse@upmc.fr

2014-02-28

We extend the previously proposed one-parameter double-hybrid density-functional theory [K. Sharkas, J. Toulouse, and A. Savin, J. Chem. Phys. 134, 064113 (2011)] to meta-generalized-gradient-approximation (meta-GGA) exchange-correlation density functionals. We construct several variants of one-parameter double-hybrid approximations using the Tao-Perdew-Staroverov-Scuseria (TPSS) meta-GGA functional and test them on test sets of atomization energies and reaction barrier heights. The most accurate variant uses the uniform coordinate scaling of the density and of the kinetic energy density in the correlation functional, and improves over both standard Kohn-Sham TPSS and second-order Møller-Plesset calculations.

Superhydrophobic Cones for Continuous Collection and Directional Transportation of CO2 Microbubbles in CO2 Supersaturated Solutions.

PubMed

Xue, Xiuzhan; Yu, Cunming; Wang, Jingming; Jiang, Lei

2016-12-27

Microbubbles are tiny bubbles with diameters below 50 μm. Because of their minute buoyant force, the microbubbles stagnate in aqueous media for a long time, and they sometimes cause serious damage. Most traditional methods chosen for elimination of gas bubbles utilize buoyancy forces including chemical methods and physical methods, and they only have a minor effect on microbubbles. Several approaches have been developed to collect and transport microbubbles in aqueous media. However, the realization of innovative strategies to directly collect and transport microbubbles in aqueous media remains a big challenge. In nature, both spider silk and cactus spines take advantage of their conical-shaped surface to yield the gradient of Laplace pressure and surface free energy for collecting fog droplets from the environment. Inspired by this, we introduce here the gradient of Laplace pressure and surface free energy to the interface of superhydrophobic copper cones (SCCs), which can continuously collect and directionally transport CO 2 microbubbles (from tip side to base side) in CO 2 -supersaturated solution. A gas layer was formed when the microbubbles encounter the SCCs. This offers a channel for microbubble directional transportation. The efficiency of microbubble transport is significantly affected by the apex angle of SCCs and the carbon dioxide concentration. The former provides different gradients of Laplace pressure as the driving force. The latter represents the capacity, which offers the quantity of CO 2 microbubbles for collection and transportation. We believe that this approach provides a simple and valid way to remove microbubbles.

Assembly of multiple cell gradients directed by three-dimensional microfluidic channels.

PubMed

Li, Yiwei; Feng, Xiaojun; Wang, Yachao; Du, Wei; Chen, Peng; Liu, Chao; Liu, Bi-Feng

2015-08-07

Active control over the cell gradient is essential for understanding biological systems and the reconstitution of the functionality of many types of tissues, particularly for organ-on-a-chip. Here, we propose a three-dimensional (3D) microfluidic strategy for generating controllable cell gradients. In this approach, a homogeneous cell suspension is loaded into a 3D stair-shaped PDMS microchannel to generate a cell gradient within 10 min by sedimentation. We demonstrate that cell gradients of various profiles (exponential and piecewise linear) can be achieved by precisely controlling the height of each layer during the fabrication. With sequential seeding, we further demonstrate the generation of two overlapping cell gradients on the same glass substrate with pre-defined designs. The cell gradient-based QD cytotoxicity assay also demonstrated that cell behaviors and resistances were regulated by the changes in cell density. These results reveal that the proposed 3D microfluidic strategy provides a simple and versatile means for establishing controllable gradients in cell density, opening up a new avenue for reconstructing functional tissues.

Direct numerical simulation of Taylor-Couette flow subjected to a radial temperature gradient

DOE Office of Scientific and Technical Information (OSTI.GOV)

Teng, Hao; Liu, Nansheng, E-mail: lns@ustc.edu.cn; Lu, Xiyun

Direct numerical simulations have been performed to study the Taylor-Couette (TC) flow between two rotating, coaxial cylinders in the presence of a radial temperature gradient. Specifically, the influence of the buoyant force and the outer cylinder rotation on the turbulent TC flow system with the radius ratio η = 0.912 was examined. For the co-rotating TC flows with Re{sub i} (inner cylinder) =1000 and Re{sub o} (outer cylinder) =100, a transition pathway to highly turbulent flows is realized by increasing σ, a parameter signifying the ratio of buoyant to inertial force. This nonlinear flow transition involves four intriguing states thatmore » emerge in sequence as chaotic wavy vortex flow for σ = 0, wavy interpenetrating spiral flows for σ = 0.02 and 0.05, intermittent turbulent spirals for σ = 0.1 and 0.2, and turbulent spirals for σ = 0.4. Overall, the fluid motion changes from a centrifugally driven flow regime characterized by large-scale wavy Taylor vortices (TVs) to a buoyancy-dominated flow regime characterized by small-scale turbulent vortices. Commensurate changes in turbulence statistics and heat transfer are seen as a result of the weakening of large-scale TV circulations and enhancement of turbulent motions. Additionally, the influence of variation of the outer cylinder rotation, −500 < Re{sub o} < 500 in presence of buoyancy (σ = 0.1) with Re{sub i} = 1000, has been considered. Specifically, it is demonstrated that this variation strongly influences the azimuthal and axial mean flows with a weaker influence on the fluctuating fluid motions. Of special interest, here are the turbulent dynamics near the outer wall where a marked decrease of turbulence intensity and a sign inversion of the Reynolds stress R{sub rz} are observed for the strongly counter-rotating regimes (Re{sub o} = − 300 and −500). To this end, it has been shown that the underlying flow physics for this drastic modification are associated with the modification of the correlation between the radial and axial fluctuating motions. In turn, the intriguing effects of this modification on the mean axial flow, turbulent statistics, force balance, and dynamic processes such as turbulence production and dissipation are discussed.« less

Density and fluence dependence of lithium cell damage and recovery characteristics

NASA Technical Reports Server (NTRS)

Faith, T. J.

1971-01-01

Experimental results on lithium-containing solar cells point toward the lithium donor density gradient dN sub L/dw as being the crucial parameter in the prediction of cell behavior after irradiation by electrons. Recovery measurements on a large number of oxygen-rich and oxygen-lean lithium cells have confirmed that cell recovery speed is directly proportional to the value of the lithium gradient for electron fluences. Gradient measurements have also been correlated with lithium diffusion schedules. Results have shown that long diffusion times (25 h) with a paint-on source result in large cell-to-cell variations in gradient, probably due to a loss of the lithium source with time.

The effect of a longitudinal density gradient on electron plasma wake field acceleration

NASA Astrophysics Data System (ADS)

Tsiklauri, David

2016-12-01

Three-dimensional, particle-in-cell, fully electromagnetic simulations of electron plasma wake field acceleration in the blow-out regime are presented. Earlier results are extended by (i) studying the effect of a longitudinal density gradient, (ii) avoiding the use of a co-moving simulation box, (iii) inclusion of ion motion, and (iv) studying fully electromagnetic plasma wake fields. It is established that injecting driving and trailing electron bunches into a positive density gradient of 10-fold increasing density over 10 cm long lithium vapour plasma results in spatially more compact and three times larger, compared with the uniform density case, electric fields (-6.4×1010 V m-1), leading to acceleration of the trailing bunch up to 24.4 GeV (starting from an initial 20.4 GeV), with energy transfer efficiencies from the leading to trailing bunch of 75%. In the uniform density case, a -2.5×1010 V m-1 wake is created leading to acceleration of the trailing bunch up to 22.4 GeV, with energy transfer efficiencies of 65%. It is also established that injecting the electron bunches into a negative density gradient of 10-fold decreasing density over 10 cm long plasma results in spatially more spread and two and a half smaller electric fields (-1.0×1010 V m-1), leading to a weaker acceleration of the trailing bunch up to 21.4 GeV, with energy transfer efficiencies of 45%. Taking ion motions into consideration shows that in the plasma wake ion number density can increase over a few times the background value. It is also shown that transverse electromagnetic fields in a plasma wake are of the same order as the longitudinal (electrostatic) ones.

Visualisation and orbital-free parametrisation of the large-Z scaling of the kinetic energy density of atoms

NASA Astrophysics Data System (ADS)

Cancio, Antonio C.; Redd, Jeremy J.

2017-03-01

The scaling of neutral atoms to large Z, combining periodicity with a gradual trend to homogeneity, is a fundamental probe of density functional theory, one that has driven recent advances in understanding both the kinetic and exchange-correlation energies. Although research focus is normally upon the scaling of integrated energies, insights can also be gained from energy densities. We visualise the scaling of the positive-definite kinetic energy density (KED) in closed-shell atoms, in comparison to invariant quantities based upon the gradient and Laplacian of the density. We notice a striking fit of the KED within the core of any atom to a gradient expansion using both the gradient and the Laplacian, appearing as an asymptotic limit around which the KED oscillates. The gradient expansion is qualitatively different from that derived from first principles for a slowly varying electron gas and is correlated with a nonzero Pauli contribution to the KED near the nucleus. We propose and explore orbital-free meta-GGA models for the kinetic energy to describe these features, with some success, but the effects of quantum oscillations in the inner shells of atoms make a complete parametrisation difficult. We discuss implications for improved orbital-free description of molecular properties.

Study of adhesion and friction properties on a nanoparticle gradient surface: transition from JKR to DMT contact mechanics.

PubMed

Ramakrishna, Shivaprakash N; Nalam, Prathima C; Clasohm, Lucy Y; Spencer, Nicholas D

2013-01-08

We have previously investigated the dependence of adhesion on nanometer-scale surface roughness by employing a roughness gradient. In this study, we correlate the obtained adhesion forces on nanometer-scale rough surfaces to their frictional properties. A roughness gradient with varying silica particle (diameter ≈ 12 nm) density was prepared, and adhesion and frictional forces were measured across the gradient surface in perfluorodecalin by means of atomic force microscopy with a polyethylene colloidal probe. Similarly to the pull-off measurements, the frictional forces initially showed a reduction with decreasing particle density and later an abrupt increase as the colloidal sphere began to touch the flat substrate beneath, at very low particle densities. The friction-load relation is found to depend on the real contact area (A(real)) between the colloid probe and the underlying particles. At high particle density, the colloidal sphere undergoes large deformations over several nanoparticles, and the contact adhesion (JKR type) dominates the frictional response. However, at low particle density (before the colloidal probe is in contact with the underlying surface), the colloidal sphere is suspended by a few particles only, resulting in local deformations of the colloid sphere, with the frictional response to the applied load being dominated by long-range, noncontact (DMT-type) interactions with the substrate beneath.

Material and Optical Densities

ERIC Educational Resources Information Center

Gluck, Paul

2007-01-01

The bending of a laser beam in a medium with a density and refractive index gradient in the same direction has been described previously. When a transparent container is half filled with a salt or sugar solution and an equal amount of water is floated on top of it, then diffusion will create a concentration gradient from top to bottom. A laser…

Particle Size Effects on CL-20 Initiation and Detonation

NASA Astrophysics Data System (ADS)

Valancius, Cole; Bainbridge, Joe; Love, Cody; Richardson, Duane

2017-06-01

Particle size or specific surface area effects on explosives has been of interest to the explosives community for both application and modeling of initiation and detonation. Different particles sizes of CL-20 were used in detonator experiments to determine the effects of particle size on initiation, run-up to steady state detonation, and steady state detonation. Historical tests have demonstrated a direct relationship between particle size and initiation. However, historical tests inadvertently employed density gradients, making it difficult to discern the effects of particle size from the effects of density. Density gradients were removed from these tests using a larger diameter, shorter charge column, allowing for similar loading across different particle sizes. Without the density gradient, the effects of particle size on initiation and detonation are easier to determine. The results of which contrast with historical results, showing particle size does not directly affect initiation threshold.

Possible influence of the Kuramoto length in a photo-catalytic water splitting reaction revealed by Poisson-Nernst-Planck equations involving ionization in a weak electrolyte

NASA Astrophysics Data System (ADS)

Suzuki, Yohichi; Seki, Kazuhiko

2018-03-01

We studied ion concentration profiles and the charge density gradient caused by electrode reactions in weak electrolytes by using the Poisson-Nernst-Planck equations without assuming charge neutrality. In weak electrolytes, only a small fraction of molecules is ionized in bulk. Ion concentration profiles depend on not only ion transport but also the ionization of molecules. We considered the ionization of molecules and ion association in weak electrolytes and obtained analytical expressions for ion densities, electrostatic potential profiles, and ion currents. We found the case that the total ion density gradient was given by the Kuramoto length which characterized the distance over which an ion diffuses before association. The charge density gradient is characterized by the Debye length for 1:1 weak electrolytes. We discuss the role of these length scales for efficient water splitting reactions using photo-electrocatalytic electrodes.

Imaging and quantification of spreading and trapping of carbon dioxide in saline aquifers using meter-scale laboratory experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Trevisan, Luca; Pini, Ronny; Cihan, Abdullah

The role of capillary forces during buoyant migration of CO 2 is critical toward plume immobilization within the postinjection phase of a geological carbon sequestration operation. However, the inherent heterogeneity of the subsurface makes it very challenging to evaluate the effects of capillary forces on the storage capacity of these formations and to assess in situ plume evolution. To overcome the lack of accurate and continuous observations at the field scale and to mimic vertical migration and entrapment of realistic CO 2 plumes in the presence of a background hydraulic gradient, we conducted two unique long-term experiments in a 2.44more » m × 0.5 m tank. X-ray attenuation allowed measuring the evolution of a CO 2 -surrogate fluid saturation, thus providing direct insight into capillarity-dominated and buoyancy-dominated flow processes occurring under successive drainage and imbibition conditions. The comparison of saturation distributions between two experimental campaigns suggests that layered-type heterogeneity plays an important role on nonwetting phase (NWP) migration and trapping, because it leads to (i) longer displacement times (3.6 months versus 24 days) to reach stable trapping conditions, (ii) limited vertical migration of the plume (with center of mass at 39% versus 55% of aquifer thickness), and (iii) immobilization of a larger fraction of injected NWP mass (67.2% versus 51.5% of injected volume) as compared to the homogenous scenario. Finally, while these observations confirm once more the role of geological heterogeneity in controlling buoyant flows in the subsurface, they also highlight the importance of characterizing it at scales that are below seismic resolution (1–10 m).« less

Imaging and quantification of spreading and trapping of carbon dioxide in saline aquifers using meter-scale laboratory experiments

DOE PAGES

Trevisan, Luca; Pini, Ronny; Cihan, Abdullah; ...

2016-12-27

The role of capillary forces during buoyant migration of CO 2 is critical toward plume immobilization within the postinjection phase of a geological carbon sequestration operation. However, the inherent heterogeneity of the subsurface makes it very challenging to evaluate the effects of capillary forces on the storage capacity of these formations and to assess in situ plume evolution. To overcome the lack of accurate and continuous observations at the field scale and to mimic vertical migration and entrapment of realistic CO 2 plumes in the presence of a background hydraulic gradient, we conducted two unique long-term experiments in a 2.44more » m × 0.5 m tank. X-ray attenuation allowed measuring the evolution of a CO 2 -surrogate fluid saturation, thus providing direct insight into capillarity-dominated and buoyancy-dominated flow processes occurring under successive drainage and imbibition conditions. The comparison of saturation distributions between two experimental campaigns suggests that layered-type heterogeneity plays an important role on nonwetting phase (NWP) migration and trapping, because it leads to (i) longer displacement times (3.6 months versus 24 days) to reach stable trapping conditions, (ii) limited vertical migration of the plume (with center of mass at 39% versus 55% of aquifer thickness), and (iii) immobilization of a larger fraction of injected NWP mass (67.2% versus 51.5% of injected volume) as compared to the homogenous scenario. Finally, while these observations confirm once more the role of geological heterogeneity in controlling buoyant flows in the subsurface, they also highlight the importance of characterizing it at scales that are below seismic resolution (1–10 m).« less

Imaging and quantification of spreading and trapping of carbon dioxide in saline aquifers using meter-scale laboratory experiments

NASA Astrophysics Data System (ADS)

Trevisan, Luca; Pini, Ronny; Cihan, Abdullah; Birkholzer, Jens T.; Zhou, Quanlin; González-Nicolás, Ana; Illangasekare, Tissa H.

2017-01-01

The role of capillary forces during buoyant migration of CO2 is critical toward plume immobilization within the postinjection phase of a geological carbon sequestration operation. However, the inherent heterogeneity of the subsurface makes it very challenging to evaluate the effects of capillary forces on the storage capacity of these formations and to assess in situ plume evolution. To overcome the lack of accurate and continuous observations at the field scale and to mimic vertical migration and entrapment of realistic CO2 plumes in the presence of a background hydraulic gradient, we conducted two unique long-term experiments in a 2.44 m × 0.5 m tank. X-ray attenuation allowed measuring the evolution of a CO2-surrogate fluid saturation, thus providing direct insight into capillarity-dominated and buoyancy-dominated flow processes occurring under successive drainage and imbibition conditions. The comparison of saturation distributions between two experimental campaigns suggests that layered-type heterogeneity plays an important role on nonwetting phase (NWP) migration and trapping, because it leads to (i) longer displacement times (3.6 months versus 24 days) to reach stable trapping conditions, (ii) limited vertical migration of the plume (with center of mass at 39% versus 55% of aquifer thickness), and (iii) immobilization of a larger fraction of injected NWP mass (67.2% versus 51.5% of injected volume) as compared to the homogenous scenario. While these observations confirm once more the role of geological heterogeneity in controlling buoyant flows in the subsurface, they also highlight the importance of characterizing it at scales that are below seismic resolution (1-10 m).

Investigation of Photographic Image Quality Estimators

DTIC Science & Technology

1980-04-01

WORDS (Conltnu* wi r« y «f •• »iä* ll n«c»*aarr «M läm’lly by ftloc* nuwWo Resolving Power Acutance SENTINEL SIGMA Math Model Modulation Transfer...Bibeman (1973) describes acutance as being "expressed in terms of the mean square of the gradient of . . . density (in a photographic image) with...the density difference AD. for each interval from the (smoothed) microdensitometer trace (calibrated in density units). 4. Compute the gradient -77

46 CFR 25.25-5 - Life preservers and other lifesaving equipment required.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Life preservers and other lifesaving equipment required... REQUIREMENTS Life Preservers and Other Lifesaving Equipment § 25.25-5 Life preservers and other lifesaving... must have at least one life preserver (Type I PFD), buoyant vest (Type II PFD), or marine buoyant...

46 CFR 25.25-5 - Life preservers and other lifesaving equipment required.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Life preservers and other lifesaving equipment required... REQUIREMENTS Life Preservers and Other Lifesaving Equipment § 25.25-5 Life preservers and other lifesaving... must have at least one life preserver (Type I PFD), buoyant vest (Type II PFD), or marine buoyant...

46 CFR 28.120 - Survival craft.

Code of Federal Regulations, 2013 CFR

2013-10-01

.... Beyond Boundary Line, within 12 miles of coastline, cold waters Less than 10.97 meters (36 feet) in... than 10.97 meters (36 feet) in length Buoyant apparatus.See note 2. Great Lakes, beyond 3 miles of... Boundary Line, within 12 miles of coastline, cold waters 10.97 meters (36 feet) or more in length Buoyant...

46 CFR 28.120 - Survival craft.

Code of Federal Regulations, 2014 CFR

2014-10-01

.... Beyond Boundary Line, within 12 miles of coastline, cold waters Less than 10.97 meters (36 feet) in... than 10.97 meters (36 feet) in length Buoyant apparatus.See note 2. Great Lakes, beyond 3 miles of... Boundary Line, within 12 miles of coastline, cold waters 10.97 meters (36 feet) or more in length Buoyant...

46 CFR 28.120 - Survival craft.

Code of Federal Regulations, 2012 CFR

2012-10-01

.... Beyond Boundary Line, within 12 miles of coastline, cold waters Less than 10.97 meters (36 feet) in... than 10.97 meters (36 feet) in length Buoyant apparatus.See note 2. Great Lakes, beyond 3 miles of... Boundary Line, within 12 miles of coastline, cold waters 10.97 meters (36 feet) or more in length Buoyant...

46 CFR 28.120 - Survival craft.

Code of Federal Regulations, 2011 CFR

2011-10-01

.... Beyond Boundary Line, within 12 miles of coastline, cold waters Less than 10.97 meters (36 feet) in... than 10.97 meters (36 feet) in length Buoyant apparatus.See note 2. Great Lakes, beyond 3 miles of... Boundary Line, within 12 miles of coastline, cold waters 10.97 meters (36 feet) or more in length Buoyant...

46 CFR 160.052-6 - Construction-nonstandard vests.

Code of Federal Regulations, 2013 CFR

2013-10-01

... the following volume of plastic foam buoyant material, determined by the displacement method: (1) Five... methods used for nonstandard buoyant vests must be equivalent to those requirements in § 160.052-5 for a... wearer in an upright or backward position with head and face out of water; (2) Have no tendency to turn a...

46 CFR 160.052-6 - Construction-nonstandard vests.

Code of Federal Regulations, 2014 CFR

2014-10-01

... the following volume of plastic foam buoyant material, determined by the displacement method: (1) Five... methods used for nonstandard buoyant vests must be equivalent to those requirements in § 160.052-5 for a... wearer in an upright or backward position with head and face out of water; (2) Have no tendency to turn a...

46 CFR 160.052-6 - Construction-nonstandard vests.

Code of Federal Regulations, 2012 CFR

2012-10-01

... the following volume of plastic foam buoyant material, determined by the displacement method: (1) Five... methods used for nonstandard buoyant vests must be equivalent to those requirements in § 160.052-5 for a... wearer in an upright or backward position with head and face out of water; (2) Have no tendency to turn a...

46 CFR 160.052-6 - Construction-nonstandard vests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... the following volume of plastic foam buoyant material, determined by the displacement method: (1) Five... methods used for nonstandard buoyant vests must be equivalent to those requirements in § 160.052-5 for a... wearer in an upright or backward position with head and face out of water; (2) Have no tendency to turn a...

46 CFR 160.060-3a - Materials-Dee ring and snap hook assemblies and other instruments of closure for buoyant vests.

Code of Federal Regulations, 2010 CFR

2010-10-01

... HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Specification for a Buoyant Vest, Unicellular Polyethylene Foam, Adult and Child § 160... and 115 pounds for a child size for 10 minutes at ambient temperatures without breaking or distorting...

46 CFR 160.064-2 - Types and models.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 6 2010-10-01 2010-10-01 false Types and models. 160.064-2 Section 160.064-2 Shipping...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Marine Buoyant Devices § 160.064-2 Types and models. (a) Types. Water safety buoyant devices covered by this subpart shall be of two general types, viz, those intended...

46 CFR 26.30-5 - Use.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., DEPARTMENT OF HOMELAND SECURITY UNINSPECTED VESSELS OPERATIONS Work Vest § 26.30-5 Use. (a) Approved buoyant work vests are considered to be items of safety apparel and may be carried aboard vessels to be worn by..., approved buoyant work vests shall not be accepted in lieu of any portion of the required number of approved...

46 CFR 26.30-5 - Use.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., DEPARTMENT OF HOMELAND SECURITY UNINSPECTED VESSELS OPERATIONS Work Vest § 26.30-5 Use. (a) Approved buoyant work vests are considered to be items of safety apparel and may be carried aboard vessels to be worn by..., approved buoyant work vests shall not be accepted in lieu of any portion of the required number of approved...

46 CFR 26.30-5 - Use.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., DEPARTMENT OF HOMELAND SECURITY UNINSPECTED VESSELS OPERATIONS Work Vest § 26.30-5 Use. (a) Approved buoyant work vests are considered to be items of safety apparel and may be carried aboard vessels to be worn by..., approved buoyant work vests shall not be accepted in lieu of any portion of the required number of approved...

46 CFR 26.30-5 - Use.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., DEPARTMENT OF HOMELAND SECURITY UNINSPECTED VESSELS OPERATIONS Work Vest § 26.30-5 Use. (a) Approved buoyant work vests are considered to be items of safety apparel and may be carried aboard vessels to be worn by..., approved buoyant work vests shall not be accepted in lieu of any portion of the required number of approved...

46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2013 CFR

2013-10-01

... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD..., inflatable life jackets, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant... other standard specified by the Commandant. (e) Repair and maintenance of inflated rescue boats must be...

46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2014 CFR

2014-10-01

... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... liferafts, inflatable buoyant apparatus, inflatable life jackets, and inflated rescue boats. (a) An... inflated rescue boats must be in accordance with the manufacturer's instructions. All repairs must be made...

46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2012 CFR

2012-10-01

... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD..., inflatable life jackets, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant... other standard specified by the Commandant. (e) Repair and maintenance of inflated rescue boats must be...

46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2013 CFR

2013-10-01

... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... liferafts, inflatable buoyant apparatus, inflatable life jackets, and inflated rescue boats. (a) An... inflated rescue boats must be in accordance with the manufacturer's instructions. All repairs must be made...

46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2014 CFR

2014-10-01

... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD..., inflatable life jackets, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant... other standard specified by the Commandant. (e) Repair and maintenance of inflated rescue boats must be...

46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2012 CFR

2012-10-01

... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... liferafts, inflatable buoyant apparatus, inflatable life jackets, and inflated rescue boats. (a) An... inflated rescue boats must be in accordance with the manufacturer's instructions. All repairs must be made...

46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2011 CFR

2011-10-01

... inflatable liferaft or inflatable buoyant apparatus must be serviced at a facility specifically approved by... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... Operational Readiness, Maintenance, and Inspection of Lifesaving Equipment § 185.730 Servicing of inflatable...

46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2010 CFR

2010-10-01

... inflatable liferaft or inflatable buoyant apparatus must be serviced at a facility specifically approved by... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... Operational Readiness, Maintenance, and Inspection of Lifesaving Equipment § 185.730 Servicing of inflatable...

46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2011 CFR

2011-10-01

... Inspection of Lifesaving Equipment § 122.730 Servicing of inflatable liferafts, inflatable buoyant apparatus... apparatus must be serviced at a facility specifically approved by the Commandant for the particular brand... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD...

46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

Code of Federal Regulations, 2010 CFR

2010-10-01

... Inspection of Lifesaving Equipment § 122.730 Servicing of inflatable liferafts, inflatable buoyant apparatus... apparatus must be serviced at a facility specifically approved by the Commandant for the particular brand... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD...

Hands-On Experiences with Buoyant-Less Water

ERIC Educational Resources Information Center

Slisko, Josip; Planinsic, Gorazd

2010-01-01

The phenomenon of weightlessness is known to students thanks to videos of amazing things astronauts do in spaceships orbiting the Earth. In this article we propose two hands-on activities which give students opportunities to infer by themselves the absence of buoyant force in a gravity accelerated system. The system is a free-falling or vertically…

Location and extent of Tertiary structures in Cook Inlet Basin, Alaska, and mantle dynamics that focus deformation and subsidence

USGS Publications Warehouse

Haeussler, Peter J.; Saltus, Richard W.

2011-01-01

Subduction of the buoyant Yakutat microplate likely caused deformation to be focused preferentially in upper Cook Inlet. The upper Cook Inlet region has both the highest degree of shortening and the deepest part of the Neogene basin. This forearc region has a long-wavelength magnetic high, a large isostatic gravity low, high conductivity in the lower mantle, low p-wave velocity (Vp), and a high p-wave to shear-wave velocity ratio (Vp/Vs). These data suggest that fluids in the mantle wedge caused serpentinization of mafic rocks, which may, at least in part, contribute to the long-wavelength magnetic anomaly. This area lies adjacent to the subducting and buoyant Yakutat microplate slab. We suggest the buoyant Yakutat slab acts much like a squeegee to focus mantle-wedge fluid flow at the margins of the buoyant slab. Such lateral flow is consistent with observed shear-wave splitting directions. The additional fluid in the adjacent mantle wedge reduces the wedge viscosity and allows greater corner flow. This results in focused subsidence, deformation, and gravity anomalies in the forearc region.

Rise of Buoyant Emissions from Low-Level Sources in the Presence of Upstream and Downstream Obstacles

NASA Astrophysics Data System (ADS)

Pournazeri, Sam; Princevac, Marko; Venkatram, Akula

2012-08-01

Field and laboratory studies have been conducted to investigate the effect of surrounding buildings on the plume rise from low-level buoyant sources, such as distributed power generators. The field experiments were conducted in Palm Springs, California, USA in November 2010 and plume rise from a 9.3 m stack was measured. In addition to the field study, a laboratory study was conducted in a water channel to investigate the effects of surrounding buildings on plume rise under relatively high wind-speed conditions. Different building geometries and source conditions were tested. The experiments revealed that plume rise from low-level buoyant sources is highly affected by the complex flows induced by buildings stationed upstream and downstream of the source. The laboratory results were compared with predictions from a newly developed numerical plume-rise model. Using the flow measurements associated with each building configuration, the numerical model accurately predicted plume rise from low-level buoyant sources that are influenced by buildings. This numerical plume rise model can be used as a part of a computational fluid dynamics model.

Cross-flow shearing effects on the trajectory of highly buoyant bent-over plumes

NASA Astrophysics Data System (ADS)

Tohidi, Ali; Kaye, Nigel Berkeley; Gollner, Michael J.

2017-11-01

The dynamics of highly buoyant plumes in cross-flow is ubiquitous throughout both industrial and environmental phenomena. The rise of smoke from a chimney, wastewater discharge into river currents, and dispersion of wildfire plumes are only a few instances. There have been many previous studies investigating the behavior of jets and highly buoyant plumes in cross-flow. So far, however, very little attention has been paid to the role of shearing effects in the boundary layer on the plume trajectory, particularly on the rise height. Numerical simulations and dimensional analysis are conducted to characterize the near- and far-field behavior of a highly buoyant plume in a boundary layer cross-flow. The results show that shear in the cross-flow leads to large differences in the rise height of the plume in relation to a uniform cross-flow, especially at far-field. This material is based upon work supported by the National Science Foundation under Grant No.1200560. Any opinions, findings, and conclusions or recommendations expressed in the material are of the authors and do not necessarily reflect the views of NSF.

Quantitative computational infrared imaging of buoyant diffusion flames

NASA Astrophysics Data System (ADS)

Newale, Ashish S.

Studies of infrared radiation from turbulent buoyant diffusion flames impinging on structural elements have applications to the development of fire models. A numerical and experimental study of radiation from buoyant diffusion flames with and without impingement on a flat plate is reported. Quantitative images of the radiation intensity from the flames are acquired using a high speed infrared camera. Large eddy simulations are performed using fire dynamics simulator (FDS version 6). The species concentrations and temperature from the simulations are used in conjunction with a narrow-band radiation model (RADCAL) to solve the radiative transfer equation. The computed infrared radiation intensities rendered in the form of images and compared with the measurements. The measured and computed radiation intensities reveal necking and bulging with a characteristic frequency of 7.1 Hz which is in agreement with previous empirical correlations. The results demonstrate the effects of stagnation point boundary layer on the upstream buoyant shear layer. The coupling between these two shear layers presents a model problem for sub-grid scale modeling necessary for future large eddy simulations.

On the derivation of empirical limits on the helium abundance in coronal holes below 1.5 solar radius

NASA Technical Reports Server (NTRS)

Habbal, Shadia Rifai; Esser, Ruth

1994-01-01

We present a simple technique describing how limits on the helium abundance, alpha, defined as the ratio of helium to proton number density, can be inferred from measurements of the electron density and temperature below 1.5 solar radius. As an illustration, we apply this technique to two different data sets: emission-line intensities in the extreme ultraviolet (EUV) and white-light observations, both measured in polar coronal holes. For the EUV data, the temperature gradient is derived from line intensity ratios, and the density gradient is replaced by the gradient of the line intensity. The lower limit on alpha derived from these data is 0.2-0.3 at 1 solar radius and drops very sharply to interplanetary values of a few percent below 1.06 solar radius. The white-light observations yield density gradients in the inner corona beyond 1.25 solar radius but do not have corresponding temperature gradients. In this case we consider an isothermal atmosphere, and derive an upper limit of 0.2 for alpha. These examples are used to illustrate how this technique could be applicable to the more extensive data to be obtained with the upcoming SOHO mission. Although only ranges on alpha can be derived, the application of the technique to data currently available merely points to the fact that alpha can be significantly large in the inner corona.

Purification of Piscirickettsia salmonis and partial characterisation of antigens

USGS Publications Warehouse

Barnes, M.N.; Landolt, M.L.; Powell, D.B.; Winton, J.R.

1998-01-01

Piscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia, an economically significant disease affecting the salmon aquaculture industry. As with other rickettsial pathogens, antigenic analysis of P. salmonis has been limited by the inherent difficulties of purifying an intracellular organism away from host cell material. In this report, we describe the use of diatrizoate meglumine and diatrizoate sodium (DMDS) density gradient centrifugation to purify P. salmonis grown in chinook salmon embryo (CHSE-214) cells. Plaque assay titers and total protein assays confirmed that viable P. salmonis was consistently concentrated in a visible band within the DMDS density gradient at a density of 1.15 to 1.16 g ml-1. Recovery of purified, viable organisms from DMDS density gradients varied from 0.6 to 3%. Preparations of uninfected CHSE-214 cells, CHSE-214 cells infected with P. salmonis, and gradient-purified P. salmonis were compared using sodium dodecyl sulfate polyacrylamide gel electrophoresis to assess the degree of purification and to identify P. salmonis-specific proteins. Although gradient-purified P. salmonis preparations were not completely free of host cell material, 8 bacterial proteins were identified. Polyclonal rabbit antiserum was used in an immunoblot of proteins from purified P. salmonis to identify 3 major and 5 minor antigens. The major antigens of 56, 30 and 20 kDa were potential candidates for experimental vaccines and development of novel diagnostic assays.

The influence of double-diffusive processes on the melting of ice in the Arctic Ocean: laboratory analogue experiments and their interpretation

NASA Astrophysics Data System (ADS)

Turner, J. S.; Veronis, G.

2004-03-01

This study has been motivated by two oceanographic observations: an increased rate of melting of sea ice in the Arctic Ocean, and the advance of an anomalously warm tongue of Atlantic water across the Arctic below the halocline over the last few decades. A series of laboratory experiments has been carried out in order to explore the physical principles underlying these phenomena, and the possibility that the extra heating at depth is responsible for the enhanced melting rate. A tank was filled with salt solution having various constant vertical density gradients. A block of ice one third of the length of the tank was floated on the surface at one end, and the rest of the surface and the walls of the tank were insulated. When no extra heat was supplied the melting rate (loss of weight of the ice in 1 h) systematically decreased as the stratification was changed from homogeneous fluid to increasingly large density gradients, while keeping the salinity of the solution in contact with the ice constant. An analogue of the intruding Atlantic water was produced by heating the lower portion of the vertical end wall at the end of the tank opposite to the ice end, keeping its temperature constant, and using the same range of salinity gradients as in the unheated experiments. Again the melting rate decreased as the density gradient was increased, but for low gradients it was larger than that in the unheated experiments. Above a certain intermediate gradient there was no significant difference in melting rate between the unheated and heated runs. The melting data were supplemented by photographs and vertical temperature and salinity profiles. The upward transfer of heat from the body of the fluid to melt the ice was clearly double-diffusive: overturning layers, separated by 'diffusive' interfaces, were visible on shadowgraphs, and the thickness of the layers decreased as the density gradient increased. The mean thickness of the layers through the depth of the tank also systematically decreased as the density gradient increased. With weak gradients an extra heat flux to the ice came from the intruding heated layer, but at large gradients this tongue of warm water at depth did not add to the flux near the surface. Though they were obtained in a simple, arbitrary and fixed geometry, we believe that the results of these experiments can be used as the basis for a better physical understanding of the melting rates of ice in the Arctic under various conditions.

An Experiment Using Sucrose Density Gradients in the Undergraduate Biochemistry Laboratory.

ERIC Educational Resources Information Center

Turchi, Sandra L.; Weiss, Monica

1988-01-01

Describes an experiment to be performed in an undergraduate biochemistry laboratory that is based on a gradient centrifugation system employing a simple bench top centrifuge, a freezer, and frozen surcose gradient solution to separate macromolecules and subcellular components. (CW)

Avifaunal responses to fire in southwestern montane forests along a burn severity gradient

USGS Publications Warehouse

Kotliar, N.B.; Kennedy, P.L.; Ferree, K.

2007-01-01

The effects of burn severity on avian communities are poorly understood, yet this information is crucial to fire management programs. To quantify avian response patterns along a burn severity gradient, we sampled 49 random plots (2001-2002) at the 17 351-ha Cerro Grande Fire (2000) in New Mexico, USA. Additionally, pre-fire avian surveys (1986-1988, 1990) created a unique opportunity to quantify avifaunal changes in 13 pre-fire transects (resampled in 2002) and to compare two designs for analyzing the effects of unplanned disturbances: after-only analysis and before-after comparisons. Distance analysis was used to calculate densities. We analyzed after-only densities for 21 species using gradient analysis, which detected a broad range of responses to increasing burn severity: (I) large significant declines, (II) weak, but significant declines, (III) no significant density changes, (IV) peak densities in low- or moderate-severity patches, (V) weak, but significant increases, and (VI) large significant increases. Overall, 71% of the species included in the after-only gradient analysis exhibited either positive or neutral density responses to fire effects across all or portions of the severity gradient (responses III-VI). We used pre/post pairs analysis to quantify density changes for 15 species using before-after comparisons; spatiotemporal variation in densities was large and confounded fire effects for most species. Only four species demonstrated significant effects of burn severity, and their densities were all higher in burned compared to unburned forests. Pre- and post-fire community similarity was high except in high-severity areas. Species richness was similar pre- and post-fire across all burn severities. Thus, ecosystem restoration programs based on the assumption that recent severe fires in Southwestern ponderosa pine forests have overriding negative ecological effects are not supported by our study of post-fire avian communities. This study illustrates the importance of quantifying burn severity and controlling confounding sources of spatiotemporal variation in studies of fire effects. After-only gradient analysis can be an efficient tool for quantifying fire effects. This analysis can also augment historical data sets that have small samples sizes coupled with high non-process variation, which limits the power of before-after comparisons. ?? 2007 by the Ecological Society of America.

Precise colloids with tunable interactions for confocal microscopy

PubMed Central

Kodger, Thomas E.; Guerra, Rodrigo E.; Sprakel, Joris

2015-01-01

Model colloidal systems studied with confocal microscopy have led to numerous insights into the physics of condensed matter. Though confocal microscopy is an extremely powerful tool, it requires a careful choice and preparation of the colloid. Uncontrolled or unknown variations in the size, density, and composition of the individual particles and interactions between particles, often influenced by the synthetic route taken to form them, lead to difficulties in interpreting the behavior of the dispersion. Here we describe the straightforward synthesis of copolymer particles which can be refractive index- and density-matched simultaneously to a non-plasticizing mixture of high dielectric solvents. The interactions between particles are accurately tuned by surface grafting of polymer brushes using Atom Transfer Radical Polymerization (ATRP), from hard-sphere-like to long-ranged electrostatic repulsion or mixed charge attraction. We also modify the buoyant density of the particles by altering the copolymer ratio while maintaining their refractive index match to the suspending solution resulting in well controlled sedimentation. The tunability of the inter-particle interactions, the low volatility of the solvents, and the capacity to simultaneously match both the refractive index and density of the particles to the fluid opens up new possibilities for exploring the physics of colloidal systems. PMID:26420044

The effects of density gradients on the convective amplification of upper hybrid waves in the magnetosphere

NASA Technical Reports Server (NTRS)

Engel, J.; Kennel, C.

1985-01-01

Intense (at least 10 mV/m) electrostatic plasma waves have been detected near the upper hybrid frequency between + or -50 deg MLAT during recent GEOS-1 crossings. Wave growth rate and convective amplification calculations were carried out in order to explain the occurrence of intense upper hybrid (IUH) events over such a wide range of latitudes. The effects of wave refractions were taken into account in the convective amplification calculations. Specific results are presented for the upper hybrid wave growth of an IUH event occurring at 10 deg MLAT. It is shown that a density gradient may be necessary to explain the observed amplification at 10 deg MLAT. At the equator, however, the long scale length of the magnetic field gradient enables large amplitudes to be attained without a density gradient. The results of a UH ray tracing analysis are discussed within the framework of current theories concerning magnetospheric continuum radiation.

Kuroshio Graduate Student Support

DTIC Science & Technology

2018-06-06

875 North Randolph Street Arlington, VA 22203-1995 11. SPONSOR/MONITOR’S REPORT NUMBER(S) 12. DISTRIBUTION / AVAILABILITY ...strong horizontal density gradients (e.g., midlatitude subtropical gyres), with a small fraction occurring in regions of deep mixed layers (e.g., high ...Society homogenous in the presence of sharp horizontal density contrasts (e.g., Fig. 1a). These sharp gradients provide a source of available

Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mortimer, Monika; Petersen, Elijah; Buchholz, Bruce

Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors. Microbes, as abundant organisms responsible for nutrient cycling in soil and water, are important ecological receptors for studying the effects of CNTs. Quantification of CNT association with microbial cells requires efficient separation of CNT-associated cells from individually dispersed CNTs and CNT agglomerates. Here in this paper, we designed, optimized, and demonstrated procedures for separating bacteria (Pseudomonas aeruginosa) from unbound multiwall carbon nanotubes (MWCNTs) and MWCNT agglomerates using sucrose density gradient centrifugation. We demonstratemore » separation of protozoa (Tetrahymena thermophila) from MWCNTs, bacterial agglomerates, and protozoan fecal pellets by centrifugation in an iodixanol solution. The presence of MWCNTs in the density gradients after centrifugation was determined by quantification of 14C-labeled MWCNTs; the recovery of microbes from the density gradient media was confirmed by optical microscopy. Protozoan intracellular contents of MWCNTs and of bacteria were also unaffected by the designed separation process. Lastly, the optimized methods contribute to improved efficiency and accuracy in quantifying MWCNT association with bacteria and MWCNT accumulation in protozoan cells, thus supporting improved assessment of CNT bioaccumulation.« less

Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation

DOE PAGES

Mortimer, Monika; Petersen, Elijah; Buchholz, Bruce; ...

2016-10-12

Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors. Microbes, as abundant organisms responsible for nutrient cycling in soil and water, are important ecological receptors for studying the effects of CNTs. Quantification of CNT association with microbial cells requires efficient separation of CNT-associated cells from individually dispersed CNTs and CNT agglomerates. Here in this paper, we designed, optimized, and demonstrated procedures for separating bacteria (Pseudomonas aeruginosa) from unbound multiwall carbon nanotubes (MWCNTs) and MWCNT agglomerates using sucrose density gradient centrifugation. We demonstratemore » separation of protozoa (Tetrahymena thermophila) from MWCNTs, bacterial agglomerates, and protozoan fecal pellets by centrifugation in an iodixanol solution. The presence of MWCNTs in the density gradients after centrifugation was determined by quantification of 14C-labeled MWCNTs; the recovery of microbes from the density gradient media was confirmed by optical microscopy. Protozoan intracellular contents of MWCNTs and of bacteria were also unaffected by the designed separation process. Lastly, the optimized methods contribute to improved efficiency and accuracy in quantifying MWCNT association with bacteria and MWCNT accumulation in protozoan cells, thus supporting improved assessment of CNT bioaccumulation.« less

Pulsed field gradients in simulations of one- and two-dimensional NMR spectra.

PubMed

Meresi, G H; Cuperlovic, M; Palke, W E; Gerig, J T

1999-03-01

A method for the inclusion of the effects of z-axis pulsed field gradients in computer simulations of an arbitrary pulsed NMR experiment with spin (1/2) nuclei is described. Recognizing that the phase acquired by a coherence following the application of a z-axis pulsed field gradient bears a fixed relation to its order and the spatial position of the spins in the sample tube, the sample is regarded as a collection of volume elements, each phase-encoded by a characteristic, spatially dependent precession frequency. The evolution of the sample's density matrix is thus obtained by computing the evolution of the density matrix for each volume element. Following the last gradient pulse, these density matrices are combined to form a composite density matrix which evolves through the rest of the experiment to yield the observable signal. This approach is implemented in a program which includes capabilities for rigorous inclusion of spin relaxation by dipole-dipole, chemical shift anisotropy, and random field mechanisms, plus the effects of arbitrary RF fields. Mathematical procedures for accelerating these calculations are described. The approach is illustrated by simulations of representative one- and two-dimensional NMR experiments. Copyright 1999 Academic Press.

SDSS-IV MaNGA: environmental dependence of stellar age and metallicity gradients in nearby galaxies

NASA Astrophysics Data System (ADS)

Zheng, Zheng; Wang, Huiyuan; Ge, Junqiang; Mao, Shude; Li, Cheng; Li, Ran; Mo, Houjun; Goddard, Daniel; Bundy, Kevin; Li, Hongyu; Nair, Preethi; Lin, Lihwai; Long, R. J.; Riffel, Rogério; Thomas, Daniel; Masters, Karen; Bizyaev, Dmitry; Brownstein, Joel R.; Zhang, Kai; Law, David R.; Drory, Niv; Roman Lopes, Alexandre; Malanushenko, Olena

2017-03-01

We present a study on the stellar age and metallicity distributions for 1105 galaxies using the STARLIGHT software on MaNGA (Mapping Nearby Galaxies at APO) integral field spectra. We derive age and metallicity gradients by fitting straight lines to the radial profiles, and explore their correlations with total stellar mass M*, NUV - r colour and environments, as identified by both the large-scale structure (LSS) type and the local density. We find that the mean age and metallicity gradients are close to zero but slightly negative, which is consistent with the inside-out formation scenario. Within our sample, we find that both the age and metallicity gradients show weak or no correlation with either the LSS type or local density environment. In addition, we also study the environmental dependence of age and metallicity values at the effective radii. The age and metallicity values are highly correlated with M* and NUV - r and are also dependent on LSS type as well as local density. Low-mass galaxies tend to be younger and have lower metallicity in low-density environments while high-mass galaxies are less affected by environment.

Protamine ratio and the level of histone retention in sperm selected from a density gradient preparation.

PubMed

Hammoud, S; Liu, L; Carrell, D T

2009-04-01

Fertile males express two forms of sperm nuclear proteins, protamine 1 (P1) and protamine 2 (P2), in roughly equal quantities, whereas some infertile men have been shown to have a reduction in protamine content and an increase in the level of histones retained in mature sperm. In this study, we assessed histone and protamine levels in spermatozoa isolated from different layers of a density gradient centrifugation column to evaluate the nuclear protein content of the sperm population selected. Protamine levels were measured using acid gel electrophoresis and immunofluorescence, and the percentage of cells retaining histones was evaluated using aniline staining and immunofluorescence. Our data suggests that there is an inverse correlation between P1/P2 ratio and the level of histone expression in the different layers of the density gradient. Paradoxically, the 90% layer had a lower P1/P2 ratio, which corresponded with an increase in histone expression. It is concluded that although the sperm population selected in the 90% layer of the density gradient columns had a lower P1/P2 ratio, it was yet similar to the P1/P2 ratio observed in previously screened fertile donors.

Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation

PubMed Central

Mortimer, Monika; Petersen, Elijah J.; Buchholz, Bruce A.; Holden, Patricia A.

2016-01-01

Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors. Microbes, as abundant organisms responsible for nutrient cycling in soil and water, are important ecological receptors for studying the effects of CNTs. Quantification of CNT association with microbial cells requires efficient separation of CNT-associated cells from individually dispersed CNTs and CNT agglomerates. Here, we designed, optimized, and demonstrated procedures for separating bacteria (Pseudomonas aeruginosa) from unbound multiwall carbon nanotubes (MWCNTs) and MWCNT agglomerates using sucrose density gradient centrifugation. We demonstrate separation of protozoa (Tetrahymena thermophila) from MWCNTs, bacterial agglomerates, and protozoan fecal pellets by centrifugation in an iodixanol solution. The presence of MWCNTs in the density gradients after centrifugation was determined by quantification of 14C-labeled MWCNTs; the recovery of microbes from the density gradient media was confirmed by optical microscopy. Protozoan intracellular contents of MWCNTs and of bacteria were also unaffected by the designed separation process. The optimized methods contribute to improved efficiency and accuracy in quantifying MWCNT association with bacteria and MWCNT accumulation in protozoan cells, thus supporting improved assessment of CNT bioaccumulation. PMID:27917301