Capillary flow enhancement in rectangular polymer microchannels with a deformable wall.

PubMed

Anoop, R; Sen, A K

2015-07-01

We report the capillary flow enhancement in rectangular polymer microchannels, when one of the channel walls is a deformable polymer membrane. We provide detailed insight into the physics of elastocapillary interaction between the capillary flow and elastic membrane, which leads to significant improvements in capillary flow performance. As liquid flows by capillary action in such channels, the deformable wall deflects inwards due to the Young-Laplace pressure drop across the liquid meniscus. This, in turn, decreases the radius of curvature of the meniscus and increases the driving capillary pressure. A theoretical model is proposed to predict the resultant increase in filling speed and rise height, respectively, in deformable horizontal and vertical microchannels having large aspect ratios. A non-dimensional parameter J, which represents the ratio of the capillary force to the mechanical restoring force, is identified to quantify the elastocapillary effects in terms of the improvement in filling speed (for J>0.238) and the condition for channel collapse (J>1). The theoretical predictions show good agreement with experimental data obtained using deformable rectangular poly(dimethylsiloxane) microchannels. Both model predictions and experimental data show that over 15% improvement in the Washburn coefficient in horizontal channels, and over 30% improvement in capillary rise height in vertical channels, are possible prior to channel collapse. The proposed technique of using deformable membranes as channel walls is a viable method for capillary flow enhancement in microfluidic devices.

Adsorption and movement of water by skin of the Australian thorny devil (Agamidae: Moloch horridus)

NASA Astrophysics Data System (ADS)

Comanns, Philipp; Esser, Falk J.; Kappel, Peter H.; Baumgartner, Werner; Shaw, Jeremy; Withers, Philip C.

2017-09-01

Moisture-harvesting lizards, such as the Australian thorny devil Moloch horridus, have remarkable adaptations for inhabiting arid regions. Their microstructured skin surface, with channels in between overlapping scales, enables them to collect water by capillarity and passively transport it to the mouth for ingestion. We characterized this capillary water transport for live thorny devils using high-speed video analyses. Comparison with preserved specimens showed that live lizards are required for detailed studies of skin water transport. For thorny devils, there was no directionality in cutaneous water transport (unlike Phrynosoma) as 7 µl water droplets applied to the skin were transported radially over more than 9.2 mm. We calculated the total capillary volume as 5.76 µl cm-2 (dorsal) and 4.45 µl cm-2 (ventral), which is reduced to 50% filling by the time transportation ceases. Using micro-computed tomography and scanning electron microscopy of shed skin to investigate capillary morphology, we found that the channels are hierarchically structured as a large channel between the scales that is sub-divided by protrusions into smaller sub-capillaries. The large channel quickly absorbs water whereas the sub-capillary structure extends the transport distance by about 39% and potentially reduces the water volume required for drinking. An adapted dynamics function, which closely reflects the channel morphology, includes that ecological role.

Adsorption and movement of water by skin of the Australian thorny devil (Agamidae: Moloch horridus).

PubMed

Comanns, Philipp; Esser, Falk J; Kappel, Peter H; Baumgartner, Werner; Shaw, Jeremy; Withers, Philip C

2017-09-01

Moisture-harvesting lizards, such as the Australian thorny devil Moloch horridus , have remarkable adaptations for inhabiting arid regions. Their microstructured skin surface, with channels in between overlapping scales, enables them to collect water by capillarity and passively transport it to the mouth for ingestion. We characterized this capillary water transport for live thorny devils using high-speed video analyses. Comparison with preserved specimens showed that live lizards are required for detailed studies of skin water transport. For thorny devils, there was no directionality in cutaneous water transport (unlike Phrynosoma ) as 7 µl water droplets applied to the skin were transported radially over more than 9.2 mm. We calculated the total capillary volume as 5.76 µl cm -2 (dorsal) and 4.45 µl cm -2 (ventral), which is reduced to 50% filling by the time transportation ceases. Using micro-computed tomography and scanning electron microscopy of shed skin to investigate capillary morphology, we found that the channels are hierarchically structured as a large channel between the scales that is sub-divided by protrusions into smaller sub-capillaries. The large channel quickly absorbs water whereas the sub-capillary structure extends the transport distance by about 39% and potentially reduces the water volume required for drinking. An adapted dynamics function, which closely reflects the channel morphology, includes that ecological role.

Nanoscale Capillary Flows in Alumina: Testing the Limits of Classical Theory.

PubMed

Lei, Wenwen; McKenzie, David R

2016-07-21

Anodic aluminum oxide (AAO) membranes have well-formed cylindrical channels, as small as 10 nm in diameter, in a close packed hexagonal array. The channels in AAO membranes simulate very small leaks that may be present for example in an aluminum oxide device encapsulation. The 10 nm alumina channel is the smallest that has been studied to date for its moisture flow properties and provides a stringent test of classical capillary theory. We measure the rate at which moisture penetrates channels with diameters in the range of 10 to 120 nm with moist air present at 1 atm on one side and dry air at the same total pressure on the other. We extend classical theory for water leak rates at high humidities by allowing for variable meniscus curvature at the entrance and show that the extended theory explains why the flow increases greatly when capillary filling occurs and enables the contact angle to be determined. At low humidities our measurements for air-filled channels agree well with theory for the interdiffusive flow of water vapor in air. The flow rate of water-filled channels is one order of magnitude less than expected from classical capillary filling theory and is coincidentally equal to the helium flow rate, validating the use of helium leak testing for evaluating moisture flows in aluminum oxide leaks.

Water imbibition by mica pores: what happens when capillary flow is suppressed?

NASA Astrophysics Data System (ADS)

Fang, Chao; Qiao, Rui

2017-11-01

The imbibition of liquids into porous media plays a critical role in numerous applications. Most prior studies focused on imbibition driven by capillary flows. In this work, we study the imbibition of water into slit-shaped mica pores filled with pressurized methane using molecular simulations. Despite that capillary flow is suppressed by the high gas pressure, water is imbibed into the pore as monolayer liquid films. Since the classical hydrodynamic flow is not readily applicable for the monolayer water film propagating on the mica wall and the imbibition is driven by the strong affinity of water molecules to the mica walls, the observed imbibition is best taken as surface hydration. We show that the dynamics of water's imbibition front follows a simple diffusive scaling law. The effective diffusion coefficient of the imbibition front, however, is more than ten times larger than the diffusion coefficient of the water molecules in the water film adsorbed on the mica walls. Using a molecular theory originally developed for the spreading of monolayer films on solid substrates, we clarify the mechanism underlying the rapid water imbibition observed here.

Demonstration of a high repetition rate capillary discharge waveguide

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gonsalves, A. J., E-mail: ajgonsalves@lbl.gov; Pieronek, C.; Daniels, J.

2016-01-21

A hydrogen-filled capillary discharge waveguide operating at kHz repetition rates is presented for parameters relevant to laser plasma acceleration (LPA). The discharge current pulse was optimized for erosion mitigation with laser guiding experiments and MHD simulation. Heat flow simulations and measurements showed modest temperature rise at the capillary wall due to the average heat load at kHz repetition rates with water-cooled capillaries, which is promising for applications of LPAs such as high average power radiation sources.

Particle sizer and DNA sequencer

DOEpatents

Olivares, Jose A.; Stark, Peter C.

2005-09-13

An electrophoretic device separates and detects particles such as DNA fragments, proteins, and the like. The device has a capillary which is coated with a coating with a low refractive index such as Teflon.RTM. AF. A sample of particles is fluorescently labeled and injected into the capillary. The capillary is filled with an electrolyte buffer solution. An electrical field is applied across the capillary causing the particles to migrate from a first end of the capillary to a second end of the capillary. A detector light beam is then scanned along the length of the capillary to detect the location of the separated particles. The device is amenable to a high throughput system by providing additional capillaries. The device can also be used to determine the actual size of the particles and for DNA sequencing.

Water Dynamics and Dewetting Transitions in the Small Mechanosensitive Channel MscS

PubMed Central

Anishkin, Andriy; Sukharev, Sergei

2004-01-01

The dynamics of confined water in capillaries and nanotubes suggests that gating of ion channels may involve not only changes of the pore geometry, but also transitions between water-filled and empty states in certain locations. The recently solved heptameric structure of the small mechanosensitive channel of Escherichia coli, MscS, has revealed a relatively wide (7–15 Å) yet highly hydrophobic transmembrane pore. Continuum estimations based on the properties of pore surface suggest low conductance and a thermodynamic possibility of dewetting. To test the predictions we performed molecular dynamics simulations of MscS filled with flexible TIP3P water. Irrespective to the initial conditions, several independent 6-ns simulations converged to the same stable state with the pore water-filled in the wider part, but predominantly empty in the narrow hydrophobic part, displaying intermittent vapor-liquid transitions. The polar gain-of-function substitution L109S in the constriction resulted in a stable hydration of the entire pore. Steered passages of Cl− ions through the narrow part of the pore consistently produced partial ion dehydration and required a force of 200–400 pN to overcome an estimated barrier of 10–20 kcal/mole, implying negligibly low conductance. We conclude that the crystal structure of MscS does not represent an open state. We infer that MscS gate, which is similar to that of the nicotinic ACh receptor, involves a vapor-lock mechanism where limited changes of geometry or surface polarity can locally switch the regime between water-filled (conducting) and empty (nonconducting) states. PMID:15111405

Transmission in situ and operando high temperature X-ray powder diffraction in variable gaseous environments

NASA Astrophysics Data System (ADS)

Schlicker, Lukas; Doran, Andrew; Schneppmüller, Peter; Gili, Albert; Czasny, Mathias; Penner, Simon; Gurlo, Aleksander

2018-03-01

This work describes a device for time-resolved synchrotron-based in situ and operando X-ray powder diffraction measurements at elevated temperatures under controllable gaseous environments. The respective gaseous sample environment is realized via a gas-tight capillary-in-capillary design, where the gas flow is achieved through an open-end 0.5 mm capillary located inside a 0.7 mm capillary filled with a sample powder. Thermal mass flow controllers provide appropriate gas flows and computer-controlled on-the-fly gas mixing capabilities. The capillary system is centered inside an infrared heated, proportional integral differential-controlled capillary furnace allowing access to temperatures up to 1000 °C.

Shapes and dynamics of miscible liquid/liquid interfaces in horizontal capillary tubes.

PubMed

Stevar, M S P; Vorobev, A

2012-10-01

We report optical observations of the dissolution behaviour of glycerol/water, soybean oil/hexane, and isobutyric acid (IBA)/water binary mixtures within horizontal capillary tubes. Tubes with diameters as small as 0.2mm were initially filled with one component of the binary mixture (solute) and then immersed into a solvent-filled thermostatic bath. Both ends of the tubes were open, and no pressure difference was applied between the ends. In the case of glycerol/water and soybean oil/hexane mixtures, we managed to isolate the dissolution (the interfacial mass transfer) from the hydrodynamic motion. Two phase boundaries moving from the ends into the middle section of the tube with the speeds v∼D(1/3)t(-2/3)d(2) (D,t and d are the coefficient of diffusion, time and the diameter of the tube, respectively) were observed. The boundaries slowly smeared but their smearing occurred considerably slower than their motion. The motion of the phase boundaries cannot be explained by the dependency of the diffusion coefficient on concentration, and should be explained by the effect of barodiffusion. The shapes of the solute/solvent boundaries are defined by the balance between gravity and surface tension effects. The contact line moved together with the bulk interface: no visible solute remained on the walls after the interface passage. Changes in temperature and in the ratio between gravity and capillary forces altered the apparent contact angles. The IBA/water system had different behaviour. Below the critical (consolute) point, no dissolution was observed: IBA and water behaved like two immiscible liquids, with the IBA phase being displaced from the tube by capillary pressure (the spontaneous imbibition process). Above the critical point, two IBA/water interfaces could be identified, however the interfaces did not penetrate much into the tube. Copyright © 2012 Elsevier Inc. All rights reserved.

Modeling capillary bridge dynamics and crack healing between surfaces of nanoscale roughness

NASA Astrophysics Data System (ADS)

Soylemez, Emrecan; de Boer, Maarten P.

2017-12-01

Capillary bridge formation between adjacent surfaces in humid environments is a ubiquitous phenomenon. It strongly influences tribological performance with respect to adhesion, friction and wear. Only a few studies, however, assess effects due to capillary dynamics. Here we focus on how capillary bridge evolution influences crack healing rates. Experimental results indicated a logarithmic decrease in average crack healing velocity as the energy release rate increases. Our objective is to model that trend. We assume that capillary dynamics involve two mechanisms: capillary bridge growth and subsequently nucleation followed by growth. We show that by incorporating interface roughness details and the presence of an adsorbed water layer, the behavior of capillary force dynamics can be understood quantitatively. We identify three important regimes that control the healing process, namely bridge growth, combined bridge growth and nucleation, and finally bridge nucleation. To fully capture the results, however, the theoretical model for nucleation time required an empirical modification. Our model enables significant insight into capillary bridge dynamics, with a goal of attaining a predictive capability for this important microelectromechanical systems (MEMS) reliability failure mechanism.

Wettability Control on Fluid-Fluid Displacements in Patterned Microfluidics

NASA Astrophysics Data System (ADS)

Zhao, B.; Trojer, M.; Cueto-Felgueroso, L.; Juanes, R.

2014-12-01

Two-phase flow in porous media is important in many natural and industrial processes like geologic CO2 sequestration, enhanced oil recovery, and water infiltration in soil. While it is well known that the wetting properties of porous media can vary drastically depending on the type of media and the pore fluids, the effect of wettability on fluid displacement continues to challenge our microscopic and macroscopic descriptions. Here we study this problem experimentally, starting with the classic experiment of two-phase flow in a capillary tube. We image the shape of the meniscus and measure the associated capillary pressure for a wide range of capillary numbers. We confirm that wettability exerts a fundamental control on meniscus deformation, and synthesize new observations on the dependence of the dynamic capillary pressure on wetting properties (contact angle) and flow conditions (viscosity contrast and capillary number). We compare our experiments to a macroscopic phase-field model of two-phase flow. We use the insights gained from the capillary tube experiments to explore the viscous fingering instability in the Hele-Shaw geometry in the partial-wetting regime. A key difference between a Hele-Shaw cell and a porous medium is the existence of micro-structures (i.e. pores and pore throats). To investigate how these micro-structrues impact fluid-fluid displacement, we conduct experiments on a planar microfluidic device patterned with vertical posts. We track the evolution of the fluid-fluid interface and elucidate the impact of wetting on the cooperative nature of fluid displacement during pore invasion events. We use the insights gained from the capillary tube and patterned microfluidics experiments to elucidate the effect of wetting properties on viscous fingering and capillary fingering in a Hele-Shaw cell filled with glass beads, where we observe a contact-angle-dependent stabilizing behavior for the emerging flow instabilities, as the system transitions from drainage to imbibition.

Impact of polymer film thickness and cavity size on polymer flow during embossing : towards process design rules for nanoimprint lithography.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schunk, Peter Randall; King, William P.; Sun, Amy Cha-Tien

2006-08-01

This paper presents continuum simulations of polymer flow during nanoimprint lithography (NIL). The simulations capture the underlying physics of polymer flow from the nanometer to millimeter length scale and examine geometry and thermophysical process quantities affecting cavity filling. Variations in embossing tool geometry and polymer film thickness during viscous flow distinguish different flow driving mechanisms. Three parameters can predict polymer deformation mode: cavity width to polymer thickness ratio, polymer supply ratio, and Capillary number. The ratio of cavity width to initial polymer film thickness determines vertically or laterally dominant deformation. The ratio of indenter width to residual film thickness measuresmore » polymer supply beneath the indenter which determines Stokes or squeeze flow. The local geometry ratios can predict a fill time based on laminar flow between plates, Stokes flow, or squeeze flow. Characteristic NIL capillary number based on geometry-dependent fill time distinguishes between capillary or viscous driven flows. The three parameters predict filling modes observed in published studies of NIL deformation over nanometer to millimeter length scales. The work seeks to establish process design rules for NIL and to provide tools for the rational design of NIL master templates, resist polymers, and process parameters.« less

Crossover from capillary fingering to viscous fingering in a rough fracture

NASA Astrophysics Data System (ADS)

Hu, R.; Chen, Y.; Wu, D. S.

2017-12-01

Controlled by the competition between capillary and viscous forces, the displacement patterns of one fluid displacing another more viscous one exhibit capillary fingering, viscous fingering, and the crossover between the two. Although extensive studies have investigated viscous and capillary fingerings in porous and fractured media, a few studies focused on the crossover in rough fractures, and how viscous and capillary forces affect the crossover remains unclear. Using a transparent fracture visualization system, we studied how the competition impacts the crossover in a horizontal rough fracture. Drainage experiments of water displacing oil were conducted at seven flow rates (capillary number log10Ca ranging from -7.07 to -3.07) and four viscosity ratios (M = 1/1000, 1/500, 1/100 and 1/50). We consistently observed lower invading fluid saturations in the crossover zone. In addition, we proposed a phase diagram for the displacement patterns in a rough fracture that is consistent with similar studies in porous media. Based on real-time imaging and statistical analysis of the invasion morphology, we showed that the competition between the capillary and viscous forces is responsible for the saturation reduction in the crossover zone. In this zone, finger propagation toward the outlet (characteristic of viscous fingering) as well as void-filling in the transverse and backward directions (characteristic of capillary fingering), are both suppressed. Therefore, the invading fluid tends to occupy larger apertures with higher characteristic front velocity, promoting void-filling toward the outlet with thinner finger growth and resulting in a larger volume of defending fluid left behind.

Invasion of gas into mica nanopores: a molecular dynamics study

NASA Astrophysics Data System (ADS)

Fang, Chao; Zhang, Fei; Qiao, Rui

2018-06-01

The invasion of gas into liquid-filled nanopores is encountered in many engineering problems but is not yet well understood. We report molecular dynamics simulations of the invasion of methane gas into water-filled mica pores with widths of 2–6 nm. Gas invades into a pore only when the pressure exceeds a breakthrough pressure and a thin residual water film is left on the mica wall as the gas phase moves deeper into the pore. The gas breakthrough pressure of pores as narrow as 2 nm can be modeled reasonably well by the capillary pressure if the finite thickness of residual liquid water film and the liquid–gas interface are taken into account. The movement of the front of the liquid meniscus during gas invasion can be quantitatively described using the classical hydrodynamics when the negative slip length on the strongly hydrophilic mica walls is taken into account. Understanding the molecular mechanisms underlying the gas invasion in the system studied here will form the foundation for quantitative prediction of gas invasion in practical porous media.

Stability of Buoyancy-Driven Gas Flow: Visualization of Coherent and Incoherent Gas Flow Patterns and Capillary Trapping

NASA Astrophysics Data System (ADS)

Geistlinger, H. W.; Samani, S.; Pohlert, M.; Jia, R.; Lazik, D.

2009-12-01

There are several mechanisms by which the CO2 can be stored: (1) In hydrodynamic trapping, the buoyant CO2 remains as a mobile fluid but is prevented from flowing back to the surface by an impermeable cap rock. (2) In solution trapping, CO2 dissolves into the brine, possibly enhanced by gravity instabilities due to the larger density of the brine-CO2 liquid mixture. (3) In mineral trapping, geochemical binding to the rock due to mineral precipitation. (4) In capillary trapping, the CO2 phase is disconnected into a coherent, mobile phase and an incoherent, immobile (trapped) phase. Recent analytical and numerical investigations [Juanes et al., 2006, 2009; Hesse et al., 2007 ] of buoyant-driven CO2-plume along a sloped aquifer are based on the following conceptual process model: (1) During the injection period, the less wetting CO2 displaces the more wetting brine in a drainage-like process. It is assumed that no capillary trapping occurs and that the CO2-network is coherent and driven both by the injection pressure and the buoyant pressure. Because of this coherence assumption a generalized Darcy-law can be used for the dynamics of the mobile, gaseous CO2-phase. (2) After injection the buoyant CO2 migrates laterally and upward, and water displaces CO2 at the trailing edge of the plume in an imbibition-like process. During this process, there are several physical mechanisms by which the water can displace the CO2 [Lenormand et al., 1983]. In addition to piston-type displacement, core-annular flow (also called: cooperative pore-body filling) may occur, i.e. the wetting phase moves along the walls and under certain conditions the CO2-core flow becomes unstable (snap-off). For water wet rocks, snap-off is the dominant mechanism [Al-Futaisi and Patzek, 2003; Valvatne and Blunt, 2004]. There seems to be consensus that the capillary trapping mechanism has a huge impact on the migration and distribution of CO2 which, in turn, affects the effectiveness of the other sequestration mechanisms. In order to investigate the stability of buoyancy-driven gas flow and the transition between coherent flow, incoherent flow, and their correlation to capillary trapping, we conducted high-resolution optical bench scale experiments. We observed a grain-size (dk) - and flow-rate (Q) dependent transition from incoherent to coherent flow. Based on core-annular flow (= cooperative pore-body filling), we propose a dynamic stability criterion that could describe our experimental results. Our experimental results for vertical gas flow support the experimental results by Lenormand et al. [1983] obtained for horizontal flow, if one takes into account that gravity leads to more unstable flow conditions. Our main results, which are in strong contradiction to the accepted conceptual model of the sloped aquifer, are: (1) Capillary Trapping can already occur during injection and at the front of the plume [Lazik et al., 2008] (2) Gas clusters or bubbles can be mobile (incoherent gas flow) and immobile (capillary trapping), and (3) Incoherent gas flow can not be described by a generalized Darcy law [Geistlinger et al., 2006, 2009].

Capillary Rise: Validity of the Dynamic Contact Angle Models.

PubMed

Wu, Pingkeng; Nikolov, Alex D; Wasan, Darsh T

2017-08-15

The classical Lucas-Washburn-Rideal (LWR) equation, using the equilibrium contact angle, predicts a faster capillary rise process than experiments in many cases. The major contributor to the faster prediction is believed to be the velocity dependent dynamic contact angle. In this work, we investigated the dynamic contact angle models for their ability to correct the dynamic contact angle effect in the capillary rise process. We conducted capillary rise experiments of various wetting liquids in borosilicate glass capillaries and compared the model predictions with our experimental data. The results show that the LWR equations modified by the molecular kinetic theory and hydrodynamic model provide good predictions on the capillary rise of all the testing liquids with fitting parameters, while the one modified by Joos' empirical equation works for specific liquids, such as silicone oils. The LWR equation modified by molecular self-layering model predicts well the capillary rise of carbon tetrachloride, octamethylcyclotetrasiloxane, and n-alkanes with the molecular diameter or measured solvation force data. The molecular self-layering model modified LWR equation also has good predictions on the capillary rise of silicone oils covering a wide range of bulk viscosities with the same key parameter W(0), which results from the molecular self-layering. The advantage of the molecular self-layering model over the other models reveals the importance of the layered molecularly thin wetting film ahead of the main meniscus in the energy dissipation associated with dynamic contact angle. The analysis of the capillary rise of silicone oils with a wide range of bulk viscosities provides new insights into the capillary dynamics of polymer melts.

Dynamic fluid connectivity during steady-state multiphase flow in a sandstone.

PubMed

Reynolds, Catriona A; Menke, Hannah; Andrew, Matthew; Blunt, Martin J; Krevor, Samuel

2017-08-01

The current conceptual picture of steady-state multiphase Darcy flow in porous media is that the fluid phases organize into separate flow pathways with stable interfaces. Here we demonstrate a previously unobserved type of steady-state flow behavior, which we term "dynamic connectivity," using fast pore-scale X-ray imaging. We image the flow of N 2 and brine through a permeable sandstone at subsurface reservoir conditions, and low capillary numbers, and at constant fluid saturation. At any instant, the network of pores filled with the nonwetting phase is not necessarily connected. Flow occurs along pathways that periodically reconnect, like cars controlled by traffic lights. This behavior is consistent with an energy balance, where some of the energy of the injected fluids is sporadically converted to create new interfaces.

Capillary flow of amorphous metal for high performance electrode

PubMed Central

Kim, Se Yun; Kim, Suk Jun; Jee, Sang Soo; Park, Jin Man; Park, Keum Hwan; Park, Sung Chan; Cho, Eun Ae; Lee, Jun Ho; Song, In Yong; Lee, Sang Mock; Han, In Taek; Lim, Ka Ram; Kim, Won Tae; Park, Ju Cheol; Eckert, Jürgen; Kim, Do Hyang; Lee, Eun-Sung

2013-01-01

Metallic glass (MG) assists electrical contact of screen-printed silver electrodes and leads to comparable electrode performance to that of electroplated electrodes. For high electrode performance, MG needs to be infiltrated into nanometer-scale cavities between Ag particles and reacts with them. Here, we show that the MG in the supercooled state can fill the gap between Ag particles within a remarkably short time due to capillary effect. The flow behavior of the MG is revealed by computational fluid dynamics and density funtional theory simulation. Also, we suggest the formation mechanism of the Ag electrodes, and demonstrate the criteria of MG for higher electrode performance. Consequently, when Al85Ni5Y8Co2 MG is added in the Ag electrodes, cell efficiency is enhanced up to 20.30% which is the highest efficiency reported so far for screen-printed interdigitated back contact solar cells. These results show the possibility for the replacement of electroplating process to screen-printing process. PMID:23851671

Capillary pumping independent of the liquid surface energy and viscosity

NASA Astrophysics Data System (ADS)

Guo, Weijin; Hansson, Jonas; van der Wijngaart, Wouter

2018-03-01

Capillary pumping is an attractive means of liquid actuation because it is a passive mechanism, i.e., it does not rely on an external energy supply during operation. The capillary flow rate generally depends on the liquid sample viscosity and surface energy. This poses a problem for capillary-driven systems that rely on a predictable flow rate and for which the sample viscosity or surface energy are not precisely known. Here, we introduce the capillary pumping of sample liquids with a flow rate that is constant in time and independent of the sample viscosity and sample surface energy. These features are enabled by a design in which a well-characterized pump liquid is capillarily imbibed into the downstream section of the pump and thereby pulls the unknown sample liquid into the upstream pump section. The downstream pump geometry is designed to exert a Laplace pressure and fluidic resistance that are substantially larger than those exerted by the upstream pump geometry on the sample liquid. Hence, the influence of the unknown sample liquid on the flow rate is negligible. We experimentally tested pumps of the new design with a variety of sample liquids, including water, different samples of whole blood, different samples of urine, isopropanol, mineral oil, and glycerol. The capillary filling speeds of these liquids vary by more than a factor 1000 when imbibed to a standard constant cross-section glass capillary. In our new pump design, 20 filling tests involving these liquid samples with vastly different properties resulted in a constant volumetric flow rate in the range of 20.96-24.76 μL/min. We expect this novel capillary design to have immediate applications in lab-on-a-chip systems and diagnostic devices.

Visualizing and quantifying the crossover from capillary fingering to viscous fingering in a rough fracture

NASA Astrophysics Data System (ADS)

Chen, Yi-Feng; Fang, Shu; Wu, Dong-Sheng; Hu, Ran

2017-09-01

Immiscible fluid-fluid displacement in permeable media is important in many subsurface processes, including enhanced oil recovery and geological CO2 sequestration. Controlled by capillary and viscous forces, displacement patterns of one fluid displacing another more viscous one exhibit capillary and viscous fingering, and crossover between the two. Although extensive studies investigated viscous and capillary fingering in porous media, a few studies focused on the crossover in rough fractures, and how viscous and capillary forces affect the crossover remains unclear. Using a transparent fracture-visualization system, we studied how the two forces impact the crossover in a horizontal rough fracture. Drainage experiments of water displacing oil were conducted at seven flow rates (capillary number log10Ca ranging from -7.07 to -3.07) and four viscosity ratios (M=1/1000,1/500,1/100 and 1/50). We consistently observed lower invading fluid saturations in the crossover zone. We also proposed a phase diagram for the displacement patterns in a rough fracture that is consistent with similar studies in porous media. Based on real-time imaging and statistical analysis of the invasion morphology, we showed that the competition between capillary and viscous forces is responsible for the saturation reduction in the crossover zone. In this zone, finger propagation toward the outlet (characteristic of viscous fingering) as well as void-filling in the transverse/backward directions (characteristic of capillary fingering), are both suppressed. Therefore, the invading fluid tends to occupy larger apertures with higher characteristic front velocity, promoting void-filling toward the outlet with thinner finger growth and resulting in a larger volume of defending fluid left behind.

Capillary filling rules and displacement mechanisms for spontaneous imbibition of CO2 for carbon storage and EOR using micro-model experiments and pore scale simulation

NASA Astrophysics Data System (ADS)

Chapman, E.; Yang, J.; Crawshaw, J.; Boek, E. S.

2012-04-01

In the 1980s, Lenormand et al. carried out their pioneering work on displacement mechanisms of fluids in etched networks [1]. Here we further examine displacement mechanisms in relation to capillary filling rules for spontaneous imbibition. Understanding the role of spontaneous imbibition in fluid displacement is essential for refining pore network models. Generally, pore network models use simple capillary filling rules and here we examine the validity of these rules for spontaneous imbibition. Improvement of pore network models is vital for the process of 'up-scaling' to the field scale for both enhanced oil recovery (EOR) and carbon sequestration. In this work, we present our experimental microfluidic research into the displacement of both supercritical CO2/deionised water (DI) systems and analogous n-decane/air - where supercritical CO2 and n-decane are the respective wetting fluids - controlled by imbibition at the pore scale. We conducted our experiments in etched PMMA and silicon/glass micro-fluidic hydrophobic chips. We first investigate displacement in single etched pore junctions, followed by displacement in complex network designs representing actual rock thin sections, i.e. Berea sandstone and Sucrosic dolomite. The n-decane/air experiments were conducted under ambient conditions, whereas the supercritical CO2/DI water experiments were conducted under high temperature and pressure in order to replicate reservoir conditions. Fluid displacement in all experiments was captured via a high speed video microscope. The direction and type of displacement the imbibing fluid takes when it enters a junction is dependent on the number of possible channels in which the wetting fluid can imbibe, i.e. I1, I2 and I3 [1]. Depending on the experiment conducted, the micro-models were initially filled with either DI water or air before the wetting fluid was injected. We found that the imbibition of the wetting fluid through a single pore is primarily controlled by the geometry of the pore body rather than the downstream pore throat sizes, contrary to the established capillary filling rules as used in current pore network models. Our experimental observations are confirmed by detailed lattice-Boltzmann pore scale computer simulations of fluid displacement in the same geometries. This suggests that capillary filling rules for imbibition as used in pore network models may need to be revised. [1] G. Lenormand, C. Zarcone and A. Sarr, J. Fluid Mech. 135 , 337-353 (1983).

Pore-scale modeling of capillary trapping in water-wet porous media: A new cooperative pore-body filling model

NASA Astrophysics Data System (ADS)

Ruspini, L. C.; Farokhpoor, R.; Øren, P. E.

2017-10-01

We present a pore-network model study of capillary trapping in water-wet porous media. The amount and distribution of trapped non-wetting phase is determined by the competition between two trapping mechanisms - snap-off and cooperative pore-body filling. We develop a new model to describe the pore-body filling mechanism in geologically realistic pore-networks. The model accounts for the geometrical characteristics of the pore, the spatial location of the connecting throats and the local fluid topology at the time of the displacement. We validate the model by comparing computed capillary trapping curves with published data for four different water-wet rocks. Computations are performed on pore-networks extracted from micro-CT images and process-based reconstructions of the actual rocks used in the experiments. Compared with commonly used stochastic models, the new model describes more accurately the experimental measurements, especially for well connected porous systems where trapping is controlled by subtleties of the pore structure. The new model successfully predicts relative permeabilities and residual saturation for Bentheimer sandstone using in-situ measured contact angles as input to the simulations. The simulated trapped cluster size distributions are compared with predictions from percolation theory.

Dynamic indocyanine green angiography measurements

NASA Astrophysics Data System (ADS)

Holmes, Timothy; Invernizzi, Alessandro; Larkin, Sean; Staurenghi, Giovanni

2012-11-01

Dynamic indocyanine green imaging uses a scanning laser ophthalmoscope and a fluorescent dye to produce movies of the dye-filling pattern in the retina and choroid of the eye. It is used for evaluating choroidal neovascularization. Movies are examined to identify the anatomy of the pathology for planning treatment and to evaluate progression or response to treatment. The popularity of this approach is affected by the complexity and difficulty in interpreting the movies. Software algorithms were developed to produce images from the movies that are easy to interpret. A mathematical model is formulated of the flow dynamics, and a fitting algorithm is designed that solves for the flow parameters. The images provide information about flow and perfusion, including regions of change between examinations. Imaged measures include the dye fill-time, temporal dispersion, and magnitude of the dye dilution temporal curves associated with image pixels. Cases show how the software can help to identify clinically relevant anatomy such as feeder vessels, drain vessels, capillary networks, and normal choroidal draining vessels. As a potential tool for research into the character of neovascular conditions and treatments, it reveals the flow dynamics and character of the lesion. Future varieties of this methodology may be used for evaluating the success of engineered tissue transplants, surgical flaps, reconstructive surgery, breast surgery, and many other surgical applications where flow, perfusion, and vascularity of tissue are important.

A novel model for simulating the racing effect in capillary-driven underfill process in flip chip

NASA Astrophysics Data System (ADS)

Zhu, Wenhui; Wang, Kanglun; Wang, Yan

2018-04-01

Underfill is typically applied in flip chips to increase the reliability of the electronic packagings. In this paper, the evolution of the melt-front shape of the capillary-driven underfill flow is studied through 3D numerical analysis. Two different models, the prevailing surface force model and the capillary model based on the wetted wall boundary condition, are introduced to test their applicability, where level set method is used to track the interface of the two phase flow. The comparison between the simulation results and experimental data indicates that, the surface force model produces better prediction on the melt-front shape, especially in the central area of the flip chip. Nevertheless, the two above models cannot simulate properly the racing effect phenomenon that appears during underfill encapsulation. A novel ‘dynamic pressure boundary condition’ method is proposed based on the validated surface force model. Utilizing this approach, the racing effect phenomenon is simulated with high precision. In addition, a linear relationship is derived from this model between the flow front location at the edge of the flip chip and the filling time. Using the proposed approach, the impact of the underfill-dispensing length on the melt-front shape is also studied.

[Is the use of plastic capillary tubes justified for blood gases analysis?].

PubMed

Daurès, Marie-Françoise; Bozonnat, Marie-Cécile; Cristol, Jean-Paul

2011-01-01

Some clinical units, such as neonatal or maternity units, preferentially use capillary tubes when analysing blood gases. Using glass tubes is delicate and nurses must recollect blood when breaking. In order to eliminate this problem, we tested flexible, plastic capillary tubes in both the above mentionned units and in our biochemistry laboratory. Each unit, where glass tubes were habitually used, tested 200 flexible, plastic capillary tubes. In addition, the nursing staffed filled out a questionnaire concerned tube usage. Both units clearly preferred using the flexible tubes. In the laboratory, results for blood gas analyses were compared between rigid glass and flexible plastic capillary tubes for 112 patients. Concordance tests did not showed significant differences between the two tube types, except for hematocrit and total haemoglobin. A questionnaire was also presented to the lab technician, who confirmed the easier usability of plastic capillary tubes.

Silicon deposition in nanopores using a liquid precursor.

PubMed

Masuda, Takashi; Tatsuda, Narihito; Yano, Kazuhisa; Shimoda, Tatsuya

2016-11-22

Techniques for depositing silicon into nanosized spaces are vital for the further scaling down of next-generation devices in the semiconductor industry. In this study, we filled silicon into 3.5-nm-diameter nanopores with an aspect ratio of 70 by exploiting thermodynamic behaviour based on the van der Waals energy of vaporized cyclopentasilane (CPS). We originally synthesized CPS as a liquid precursor for semiconducting silicon. Here we used CPS as a gas source in thermal chemical vapour deposition under atmospheric pressure because vaporized CPS can fill nanopores spontaneously. Our estimation of the free energy of CPS based on Lifshitz van der Waals theory clarified the filling mechanism, where CPS vapour in the nanopores readily undergoes capillary condensation because of its large molar volume compared to those of other vapours such as water, toluene, silane, and disilane. Consequently, a liquid-specific feature was observed during the deposition process; specifically, condensed CPS penetrated into the nanopores spontaneously via capillary force. The CPS that filled the nanopores was then transformed into solid silicon by thermal decomposition at 400 °C. The developed method is expected to be used as a nanoscale silicon filling technology, which is critical for the fabrication of future quantum scale silicon devices.

Silicon deposition in nanopores using a liquid precursor

NASA Astrophysics Data System (ADS)

Masuda, Takashi; Tatsuda, Narihito; Yano, Kazuhisa; Shimoda, Tatsuya

2016-11-01

Techniques for depositing silicon into nanosized spaces are vital for the further scaling down of next-generation devices in the semiconductor industry. In this study, we filled silicon into 3.5-nm-diameter nanopores with an aspect ratio of 70 by exploiting thermodynamic behaviour based on the van der Waals energy of vaporized cyclopentasilane (CPS). We originally synthesized CPS as a liquid precursor for semiconducting silicon. Here we used CPS as a gas source in thermal chemical vapour deposition under atmospheric pressure because vaporized CPS can fill nanopores spontaneously. Our estimation of the free energy of CPS based on Lifshitz van der Waals theory clarified the filling mechanism, where CPS vapour in the nanopores readily undergoes capillary condensation because of its large molar volume compared to those of other vapours such as water, toluene, silane, and disilane. Consequently, a liquid-specific feature was observed during the deposition process; specifically, condensed CPS penetrated into the nanopores spontaneously via capillary force. The CPS that filled the nanopores was then transformed into solid silicon by thermal decomposition at 400 °C. The developed method is expected to be used as a nanoscale silicon filling technology, which is critical for the fabrication of future quantum scale silicon devices.

Capillary electrochromatography and capillary electrochromatography-electrospray mass spectrometry for the separation of non-steroidal anti-inflammatory drugs.

PubMed

Desiderio, C; Fanali, S

2000-10-20

In this study capillary electrochromatography (CEC) was utilized for the separation of ten non-steroidal anti-inflammatory drugs (NSAIDs). Experiments were carried out in a commercially available CE instrument using a packed capillary with RP-18 silica particles where the stationary phase completely filled the capillary. The mobile phase consisted of a mixture of ammonium formate buffer pH 2.5 and acetonitrile. Selectivity and resolution were studied changing the pH and the concentration of the buffer, the acetonitrile content mobile phase and the capillary temperature. The optimum experimental conditions for CEC separation of the studied drug mixture were found using 50 mM ammonium formate pH 2.5-acetonitrile (40:60) at 25 degrees C. The CEC capillary was coupled to an electrospray mass spectrometer for the characterization of the NSAIDs. A mobile phase composed by the same buffer but with a higher concentration of acetonitrile (90%) was used in order to speed up the separation of analytes.

Laser beam coupling with capillary discharge plasma for laser wakefield acceleration applications

NASA Astrophysics Data System (ADS)

Bagdasarov, G. A.; Sasorov, P. V.; Gasilov, V. A.; Boldarev, A. S.; Olkhovskaya, O. G.; Benedetti, C.; Bulanov, S. S.; Gonsalves, A.; Mao, H.-S.; Schroeder, C. B.; van Tilborg, J.; Esarey, E.; Leemans, W. P.; Levato, T.; Margarone, D.; Korn, G.

2017-08-01

One of the most robust methods, demonstrated to date, of accelerating electron beams by laser-plasma sources is the utilization of plasma channels generated by the capillary discharges. Although the spatial structure of the installation is simple in principle, there may be some important effects caused by the open ends of the capillary, by the supplying channels etc., which require a detailed 3D modeling of the processes. In the present work, such simulations are performed using the code MARPLE. First, the process of capillary filling with cold hydrogen before the discharge is fired, through the side supply channels is simulated. Second, the simulation of the capillary discharge is performed with the goal to obtain a time-dependent spatial distribution of the electron density near the open ends of the capillary as well as inside the capillary. Finally, to evaluate the effectiveness of the beam coupling with the channeling plasma wave guide and of the electron acceleration, modeling of the laser-plasma interaction was performed with the code INF&RNO.

Capillary reference half-cell

DOEpatents

Hall, Stephen H.

1996-01-01

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods.

Capillary reference half-cell

DOEpatents

Hall, S.H.

1996-02-13

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods. 11 figs.

Finite volume solution for two-phase flow in a straight capillary

NASA Astrophysics Data System (ADS)

Yelkhovsky, Alexander; Pinczewski, W. Val

2018-04-01

The problem of two-phase flow in straight capillaries of polygonal cross section displays many of the dynamic characteristics of rapid interfacial motions associated with pore-scale displacements in porous media. Fluid inertia is known to be important in these displacements but is usually ignored in network models commonly used to predict macroscopic flow properties. This study presents a numerical model for two-phase flow which describes the spatial and temporal evolution of the interface between the fluids. The model is based on an averaged Navier-Stokes equation and is shown to be successful in predicting the complex dynamics of both capillary rise in round capillaries and imbibition along the corners of polygonal capillaries. The model can form the basis for more realistic network models which capture the effect of capillary, viscous, and inertial forces on pore-scale interfacial dynamics and consequent macroscopic flow properties.

Fetal villosity and microvasculature of the bovine placentome in the second half of gestation

PubMed Central

LEISER, R.; KREBS, C.; KLISCH, K.; EBERT, B.; DANTZER, V.; SCHULER, G.; HOFFMANN, B.

1997-01-01

The architecture of the fetal villous tree and its vasculature in the bovine placentome were studied in the second half of gestation using both conventional histology and histology of ink-filled blood vessels. These were compared with corrosion casts of plastic fillings of the vasculature, prepared for scanning electron microscopy. This combination of morphological methods allows perception of the villous tree throughout gestation from broad-conical to tall-conical form where branch ramification occurs mainly at right angles to the stem. The stem villus typically contains a single central artery and several peripheral veins arranged in parallel. The proximal branches to the stem, the intermediate villi, contain a central arteriole and accompanying venules. The distal branches, the terminal villi, enclose capillary convolutions which consist of an afferent arterial capillary limb, capillary loops and efferent venous capillary limbs. Vascular interconnections exist within the terminal villi, as capillaries or venules between the capillary convolutions, serially bridging them in up to 5 places, and as capillary anastomoses between the capillary loops. Coiling and sinusoidal dilatations of these loops develop near the end of gestation. The intraplacentomal rearrangement of villous trees with progressive gestation and their morphological vascular adaptations are discussed in relation to placental function, including the ever increasing need for transplacental substance exchange. This adaptation allows the blood to traverse the shortest possible arterioarteriolar route to the periphery of the trees where exchange takes place. The need for an increasing blood flow stimulates capillary growth and at the same time optimises the blood flow reaching the placental barrier represented by the vessel cast surface. The capillaries also carry the blood back into the very voluminous system of venules and veins where back diffusion may occur. The total volume of terminal villi of bovine placentome, the ‘working part’ of villous trees, hence distinctly increases with respect to the stem and intermediate villi, the ‘supplying part’ of the villous tree. In morphological terms the efficiency of the bovine transplacental diffusional exchange is higher than in the closely related ‘co-ruminants’ sheep and goats and distinctly higher when compared with the human placenta. PMID:9449071

Mathematical Model of Bubble Sloshing Dynamics for Cryogenic Liquid Helium in Orbital Spacecraft Dewar Container

NASA Technical Reports Server (NTRS)

Hung, R. J.; Pan, H. L.

1995-01-01

A generalized mathematical model is investigated of sloshing dynamics for dewar containers, partially filled with a liquid of cryogenic superfluid helium 2, driven by both gravity gradient and jitter accelerations applicable to two types of scientific spacecrafts, which are eligible to carry out spinning motion and/or slew motion to perform scientific observations during normal spacecraft operation. Two examples are given for the Gravity Probe-B (GP-B) with spinning motion, and the Advanced X-Ray Astrophysics Facility-Spectroscopy (AXAF-S) with slew motion, which are responsible for the sloshing dynamics. Explicit mathematical expressions for the modelling of sloshing dynamics to cover these forces acting on the spacecraft fluid systems are derived. The numerical computation of sloshing dynamics will be based on the noninertial frame spacecraft bound coordinate, and we will solve the time-dependent three-dimensional formulations of partial differential equations subject to initial and boundary conditions. Explicit mathematical expressions of boundary conditions lo cover capillary force effects on the liquid-vapor interface in microgravity environments are also derived. Results of the simulations of the mathematical model are illustrated.

Low Volume Resuscitation with Cell Impermeants

DTIC Science & Technology

2016-04-01

to rise) and a fall in peripheral vascular resistance . In this model, hemorrhage and blood loss was controlled so any changes in hemoglobin...appealing. The increase in capillary filling together with reduced resistance to flow in these peripheral beds leads to increased blood flow and oxygen...delivery. The low resistance , compared to saline controls, likely represents a physical decompression of the capillary beds by controlling cell and

Capillary descent.

PubMed

Delannoy, Joachim; de Maleprade, Hélène; Clanet, Christophe; Quéré, David

2018-05-31

A superhydrophobic capillary tube immersed in water and brought in contact with the bath surface will be invaded by air, owing to its aerophilicity. We discuss this phenomenon where the ingredients of classical capillary rise are inverted, which leads to noticeable dynamical features. (1) The main regime of air invasion is linear in time, due to the viscous resistance of water. (2) Menisci in tubes with millimetre-size radii strongly oscillate before reaching their equilibrium depth, a consequence of inertia. On the whole, capillary descent provides a broad variety of dynamics where capillary effects, viscous friction and liquid inertia all play a role.

Nonlinear model and attitude dynamics of flexible spacecraft with large amplitude slosh

NASA Astrophysics Data System (ADS)

Deng, Mingle; Yue, Baozeng

2017-04-01

This paper is focused on the nonlinearly modelling and attitude dynamics of spacecraft coupled with large amplitude liquid sloshing dynamics and flexible appendage vibration. The large amplitude fuel slosh dynamics is included by using an improved moving pulsating ball model. The moving pulsating ball model is an equivalent mechanical model that is capable of imitating the whole liquid reorientation process. A modification is introduced in the capillary force computation in order to more precisely estimate the settling location of liquid in microgravity or zero-g environment. The flexible appendage is modelled as a three dimensional Bernoulli-Euler beam and the assumed modal method is employed to derive the nonlinear mechanical model for the overall coupled system of liquid filled spacecraft with appendage. The attitude maneuver is implemented by the momentum transfer technique, and a feedback controller is designed. The simulation results show that the liquid sloshing can always result in nutation behavior, but the effect of flexible deformation of appendage depends on the amplitude and direction of attitude maneuver performed by spacecraft. Moreover, it is found that the liquid sloshing and the vibration of flexible appendage are coupled with each other, and the coupling becomes more significant with more rapid motion of spacecraft. This study reveals that the appendage's flexibility has influence on the liquid's location and settling time in microgravity. The presented nonlinear system model can provide an important reference for the overall design of the modern spacecraft composed of rigid platform, liquid filled tank and flexible appendage.

Shifts in pore connectivity from precipitation versus groundwater rewetting increases soil carbon loss after drought

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, A. Peyton; Bond-Lamberty, Ben; Benscoter, Brian W.

Droughts and other extreme precipitation events are predicted to increase in intensity, duration and extent, with uncertain implications for terrestrial carbon (C) sequestration. Soil wetting from above (precipitation) results in a characteristically different pattern of pore-filling than wetting from below (groundwater), with larger, well-connected pores filling before finer pore spaces, unlike groundwater rise in which capillary forces saturate the finest pores first. Here we demonstrate that pore-scale wetting patterns interact with antecedent soil moisture conditions to alter pore-, core- and field-scale C dynamics. Drought legacy and wetting direction are perhaps more important determinants of short-term C mineralization than current soilmore » moisture content in these soils. Our results highlight that microbial access to C is not solely limited by physical protection, but also by drought or wetting-induced shifts in hydrologic connectivity. We argue that models should treat soil moisture within a three-dimensional framework emphasizing hydrologic conduits for C and resource diffusion.« less

Method of filling a microchannel separation column

DOEpatents

Arnold, Don W.

2002-01-01

A method for packing a stationary phase into a small diameter fluid passageway or flow channel. Capillary action is employed to distribute a stationary phase uniformly along both the length and diameter of the flow channel. The method disclosed here: 1) eliminates the need for high pressure pumps and fittings and the safety hazards associated therewith; 2) allows the use of readily available commercial microparticles, either coated or uncoated, as the stationary phase; 3) provides for different types of particles, different particle sizes, and different particle size distributions to be packed in sequence, or simultaneously; 4) eliminates the need for plugging the flow channel prior to adding the stationary phase to retain the packing particles; and 5) many capillaries can be filled simultaneously.

Effects of aperture variability and wettability on immiscible displacement in fractures

NASA Astrophysics Data System (ADS)

Yang, Zhibing; Méheust, Yves; Neuweiler, Insa

2017-04-01

Fluid-fluid displacement in porous and fractured media is an important process. Understanding and controlling this process is key to many practical applications, such as hydrocarbon recovery, geological storage of CO2, groundwater remediation, etc. Here, we numerically study fluid-fluid displacement in rough-walled fractures. We focus on the combined effect of wettability and fracture surface topography on displacement patterns and interface growth. We develop a novel numerical model to simulate dynamic fluid invasion under the influence of capillary and viscous forces. The capillary force is calculated using the two principal curvatures (aperture-induced curvature and in-plane curvature) at the fluid-fluid interface, and the viscous force is taken into account by solving the fluid pressure distribution. The aperture field of a fracture is represented by a spatially correlated random field, which is described by a power spectrum for the fracture wall topography and a cutoff wave-length. We numerically produce displacement patterns ranging from stable displacement, capillary fingering, and viscous fingering, as well as the transitions between them. We show that both reducing the aperture variability and increasing the contact angle (from drainage to weak imbibition) stabilize the displacement due to the influence of the in-plane curvature, an effect analogous to that of the cooperative pore filling in porous media. Implications of these results will be discussed.

Capillary-Effect Root-Environment System

NASA Technical Reports Server (NTRS)

Wright, Bruce D.

1991-01-01

Capillary-effect root-environment system (CERES) is experimental apparatus for growing plants in nutrient solutions. Solution circulated at slight tension in cavity filled with plastic screen and covered by porous plastic membrane. By adsorptive attraction, root draws solution through membrane. Conceived for use in microgravity of space, also finds terrestrial application in germinating seedlings, because it protects them from extremes of temperature, moisture, and soil pH and from overexposure to fertilizers and herbicides.

Into the theory of the partial-filling affinity capillary electrophoresis and the determination of apparent stability constants of analyte-ligand complexes.

PubMed

Ansorge, Martin; Dubský, Pavel; Ušelová, Kateřina

2018-03-01

The partial-filling affinity capillary electrophoresis (pf-ACE) works with a ligand present in a background electrolyte that forms a weak complex with an analyte. In contrast to a more popular mobility-shift affinity capillary electrophoresis, only a short plug of the ligand is introduced into a capillary in the pf-ACE. Both methods can serve for determining apparent stability constants of the formed complexes but this task is hindered in the pf-ACE by the fact that the analyte spends only a part of its migration time in a contact with the ligand. In 1998, Amini and Westerlund published a linearization strategy that allows for extracting an effective mobility of an analyte in the presence of a neutral ligand out of the pf-ACE data. The main purpose of this paper is to show that the original formula is only approximate. We derive a new formula and demonstrate its applicability by means of computer simulations. We further inspect several strategies of data processing in the pf-ACE regarding a risk of an error propagation. This establishes a good practice of determining apparent stability constants of analyte-ligand complexes by means of the pf-ACE. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Method development of enantiomer separations by affinity capillary electrophoresis, cyclodextrin electrokinetic chromatography and capillary electrophoresis-mass spectrometry.

PubMed

Tanaka, Yoshihide

2002-07-01

Capillary electrophoresis (CE) has become a powerful tool for enantiomer separations during the last decade. Since 1993, the author has investigated enantiomer separations by affinity capillary electrophoresis (affinity CE) with some proteins and by cyclodextrin electrokinetic chromatography (CDEKC) with some charged cyclodextrins (CDs). Many successful enantiomer separations are demonstrated from our study in this review article. In the enantiomer separations by affinity CE, the deterioration of detection sensitivity was observed under high concentration of the protein in running solutions. The partial filling technique was practically useful to solve the serious problem. It allowed operation at high protein concentrations, such as 500 mumol/L, without the detection problem. Charged CDs had several advantages for the enantiomer separations over neutral ones. Strong electrostatic interactions between a charged CD and oppositely charged analytes should be effective for the formation of the complex. A large difference in electrophoretic mobility between the free analyte and the inclusion complex should also enhance the enantiomeric resolution. In CE-mass spectrometry (CE-MS), the partial filling technique was applied to avoid the introduction of nonvolatile chiral selectors into the CE-MS interface. By replacing the nonvolatile electrolytes in the running buffer by volatile ones, the separation conditions employed in CE with the UV detection method could be transferred to CE-MS.

Dynamical behavior of surface tension on rotating fluids in low and microgravity environments

NASA Technical Reports Server (NTRS)

Hung, R. J.; Tsao, Y. D.; Hong, B. B.; Leslie, F. W.

1989-01-01

Consideration is given to the time-dependent evolutions of the free surface profile (bubble shapes) of a cylindrical container, partially filled with a Newtonian fluid of constant density, rotating about its axis of symmetry in low and microgravity environments. The dynamics of the bubble shapes are calculated for four cases: linear time-dependent functions of spin-up and spin-down in low and microgravity, linear time-dependent functions of increasing and decreasing gravity at high and low rotating cylinder speeds, time-dependent step functions of spin-up and spin-down in low gravity, and sinusoidal function oscillation of the gravity environment in high and low rotating cylinder speeds. It is shown that the computer algorithms developed by Hung et al. (1988) may be used to simulate the profile of time-dependent bubble shapes under variations of centrifugal, capillary, and gravity forces.

Pulmonary diffusing capacity, capillary blood volume, and cardiac output during sustained microgravity

NASA Technical Reports Server (NTRS)

Prisk, G. K.; Guy, Harold J. B.; Elliott, Ann R.; Deutschman, Robert A., III; West, John B.

1993-01-01

We measured pulmonary diffusing capacity (DL), diffusing capacity per unit lung volume, pulmonary capillary blood volume (Vc), membrane diffusing capacity (Dm), pulmonary capillary blood flow or cardiac output (Qc), and cardiac stroke volume (SV) in four subjects exposed to nine days of microgravity. DL in microgravity was elevated compared with preflight standing values and was higher than preflight supine because of the elevation of both Vc and Dm. The elevation in Vc was comparable to that measured supine in 1 G, but the increase in Dm was in sharp contrast to the supine value. We postulate that, in 0 G, pulmonary capillary blood is evenly distributed throughout the lung, providing for uniform capillary filling, leading to an increase in the surface area available for diffusion. By contrast, in the supine 1-G state, the capillaries are less evenly filled, and although a similar increase in blood volume is observed, the corresponding increase in surface area does not occur. DL and its subdivisions showed no adaptive changes from the first measurement 24 h after the start of 0 G to eight days later. Similarly, there were no trends in the postflight data, suggesting that the principal mechanism of these changes was gravitational. The increase in Dm suggests that subclinical pulmonary edema did not result from exposure to 0 G. Qc was modestly increased inflight and decreased postflight compared with preflight standing. Compared with preflight standing, SV was increased 46 percent inflight and decreased 14 percent in the 1st week postflight. There were temporal changes in Qc and SV during 0 G, with the highest values recorded at the first measurement, 24 h into the flight. The lowest values of Qc and SV occurred on the day of return.

A many-body dissipative particle dynamics study of forced water-oil displacement in capillary.

PubMed

Chen, Chen; Zhuang, Lin; Li, Xuefeng; Dong, Jinfeng; Lu, Juntao

2012-01-17

The forced water-oil displacement in capillary is a model that has important applications such as the groundwater remediation and the oil recovery. Whereas it is difficult for experimental studies to observe the displacement process in a capillary at nanoscale, the computational simulation is a unique approach in this regard. In the present work, the many-body dissipative particle dynamics (MDPD) method is employed to simulate the process of water-oil displacement in capillary with external force applied by a piston. As the property of all interfaces involved in this system can be manipulated independently, the dynamic displacement process is studied systematically under various conditions of distinct wettability of water in capillary and miscibility between water and oil as well as of different external forces. By analyzing the dependence of the starting force on the properties of water/capillary and water/oil interfaces, we find that there exist two different modes of the water-oil displacement. In the case of stronger water-oil interaction, the water particles cannot displace those oil particles sticking to the capillary wall, leaving a low oil recovery efficiency. To minimize the residual oil content in capillary, enhancing the wettability of water and reducing the external force will be beneficial. This simulation study provides microscopic insights into the water-oil displacement process in capillary and guiding information for relevant applications. © 2011 American Chemical Society

Intermittent KoldBlue cryotherapy of 3x10 min changes mid-portion Achilles tendon microcirculation.

PubMed

Knobloch, Karsten; Grasemann, Ruth; Spies, Marcus; Vogt, Peter M

2007-06-01

Neovascularisation and microcirculatory changes have been reported in Achilles tendinopathy. Cryotherapy and compression, as part of a rest, ice, compression and elevation regimen, are shown to decrease pain and improve function. However, the microcirculatory changes following a given dosage of cryotherapy on mid-portion Achilles tendon remain unclear. Prospective clinical cohort study, level of evidence 2. 30 people (12 males, 33 (SD 12) years, body mass index 25.6 (5.3) kg/m2) were included in the cohort. 3x10 min KoldBlue ankle-cooling bandages were applied and microcirculation of Achilles tendon mid-portion was real-time and continuously assessed using a laser-Doppler-spectrophotometry system (O2C, Germany). Superficial capillary blood flow was reduced from 42 to 6, 5 and 3 relative units (rU) in the first, second and third cryotherapy periods, respectively (-65%, p = 0.001), with no significant capillary hyperaemia. Deep capillary tendon blood flow was reduced from 180 to 82, 53 and 52 rU (-71%, p = 0.001) within 6-9 min of application without hyperaemia. Superficial tendon oxygen saturation dropped significantly from 43% to 26%, 18% and 11% (p = 0.001) after repetitive cryotherapy, with persisting increase of tendon oxygenation during rewarming (51%, 49% and 54%, p = 0.077) up to 27% of the baseline level. At 8 mm tendon depth, cryotherapy preserved local oxygenation. Relative postcapillary venous tendon filling pressures were favourably reduced from 41 (11) to 31, 28 and 26 rU (-36%, p = 0.001) superficially and from 56 (11) to 45, 46 and 48 rU (-18%, p = 0.001) in deep capillary blood flow during cryotherapy, facilitating capillary venous clearance. Intermittent cryotherapy of 3x10 min significantly decreases local Achilles tendon mid-portion capillary blood flow by 71%. Within 2 min of rewarming, tendon oxygen saturation is re-established following cryotherapy. Postcapillary venous filling pressures are reduced during cryotherapy, favouring capillary venous outflow of the healthy Achilles tendon.

Optoelectronic Capillary Sensors in Microfluidic and Point-of-Care Instrumentation

PubMed Central

Borecki, Michał; Korwin-Pawlowski, Michael L.; Beblowska, Maria; Szmidt, Jan; Jakubowski, Andrzej

2010-01-01

This paper presents a review, based on the published literature and on the authors’ own research, of the current state of the art of fiber-optic capillary sensors and related instrumentation as well as their applications, with special emphasis on point-of-care chemical and biochemical sensors, systematizing the various types of sensors from the point of view of the principles of their construction and operation. Unlike classical fiber-optic sensors which rely on changes in light propagation inside the fiber as affected by outside conditions, optical capillary sensors rely on changes of light transmission in capillaries filled with the analyzed liquid, which opens the possibility of interesting new applications, while raising specific issues relating to the construction, materials and instrumentation of those sensors. PMID:22319325

Capillary Tube and Thermostatic Expansion Valve Comparative Analysis in Water Chiller Air Conditioning

NASA Astrophysics Data System (ADS)

Wijaya Sunu, Putu; Made Rasta, I.; Anakottapary, Daud Simon; Made Suarta, I.; Cipta Santosa, I. D. M.

2018-01-01

The aims of this study to compares the performance characteristics of a water chiller air conditioning simulation equipped with thermostatic expansion valve (TEV) with those of a capillary tube. Water chiller system filled with the same charge of refrigerant. Comparative analyses were performed based on coefficient of performance (COP) and performance parameter of the refrigeration system, carried out at medium cooling load level with the ambient temperature of 29-31°C, constant compressor speed and fixed chilled water volume flowrate at 15 lpm. It was shown that the TEV system showed better energy consumption compared to that of capillary tube. From the coefficient of performance perspective, the thermostatic expansion valve system showed higher COP (± 21.4%) compared to that of capillary tube system.

Control of electroosmosis in coated quartz capillaries

NASA Technical Reports Server (NTRS)

Herren, Blair J.; Van Alstine, James; Snyder, Robert S.; Shafer, Steven G.; Harris, J. Milton

1987-01-01

The effectiveness of various coatings for controlling the electroosmotic fluid flow that hinders electrophoretic processes is studied using analytical particle microelectrophoresis. The mobilities of 2-micron diameter glass and polystyrene latex spheres (exhibiting both negative and zero effective surface charge) were measured in 2-mm diameter quartz capillaries filled with NaCl solutions within the 3.5-7.8 pH range. It is found that capillary inner surface coatings using 5000 molecular weight (or higher) poly(ethylene glycol): significantly reduced electroosmosis within the selected pH range, were stable for long time periods, and appeared to be more effective than dextran, methylcellulose, or silane coatings.

Effect of hindpaw electrical stimulation on capillary flow heterogeneity and oxygen delivery (Conference Presentation)

NASA Astrophysics Data System (ADS)

Li, Yuandong; Wei, Wei; Li, Chenxi; Wang, Ruikang K.

2017-02-01

We report a novel use of optical coherence tomography (OCT) based angiography to visualize and quantify dynamic response of cerebral capillary flow pattern in mice upon hindpaw electrical stimulation through the measurement of the capillary transit-time heterogeneity (CTH) and capillary mean transit time (MTT) in a wide dynamic range of a great number of vessels in vivo. The OCT system was developed to have a central wavelength of 1310 nm, a spatial resolution of 8 µm and a system dynamic range of 105 dB at an imaging rate of 92 kHz. The mapping of dynamic cerebral microcirculations was enabled by optical microangiography protocol. From the imaging results, the spatial homogenization of capillary velocity (decreased CTH) was observed in the region of interest (ROI) corresponding to the stimulation, along with an increase in the MTT in the ROI to maintain sufficient oxygen exchange within the brain tissue during functional activation. We validated the oxygen consumption due to an increase of the MTT through demonstrating an increase in the deoxygenated hemoglobin (HbR) during the stimulation by the use of laser speckle contrast imaging.

Harvesting liquid from unsaturated vapor - nanoflows induced by capillary condensation

NASA Astrophysics Data System (ADS)

Vincent, Olivier; Marguet, Bastien; Stroock, Abraham

2016-11-01

A vapor, even subsaturated, can spontaneously form liquid in nanoscale spaces. This process, known as capillary condensation, plays a fundamental role in various contexts, such as the formation of clouds or the dynamics of hydrocarbons in the geological subsurface. However, large uncertainties remain on the thermodynamics and fluid mechanics of the phenomenon, due to experimental challenges as well as outstanding questions about the validity of macroscale physics at the nanometer scale. We studied experimentally the spatio-temporal dynamics of water condensation in a model nanoporous medium (pore radius 2 nm), taking advantage of the color change of the material upon hydration. We found that at low relative humidities (< 60 % RH), capillary condensation progressed in a diffusive fashion, while it occurred through a well-defined capillary-viscous imbibition front at > 60 % RH, driven by a balance between the pore capillary pressure and the condensation stress given by Kelvin equation. Further analyzing the imbibition dynamics as a function of saturation allowed us to extract detailed information about the physics of nano-confined fluids. Our results suggest excellent extension of macroscale fluid dynamics and thermodynamics even in pores 10 molecules in diameter.

Partitioning dynamics of unsaturated flows in fractured porous media: Laboratory studies and three-dimensional multi-scale smoothed particle hydrodynamics simulations of gravity-driven flow in fractures

NASA Astrophysics Data System (ADS)

Kordilla, J.; Bresinsky, L. T.; Shigorina, E.; Noffz, T.; Dentz, M.; Sauter, M.; Tartakovsky, A. M.

2017-12-01

Preferential flow dynamics in unsaturated fractures remain a challenging topic on various scales. On pore- and fracture-scales the highly erratic gravity-driven flow dynamics often provoke a strong deviation from classical volume-effective approaches. Against the common notion that flow in fractures (or macropores) can only occur under equilibrium conditions, i.e., if the surrounding porous matrix is fully saturated and capillary pressures are high enough to allow filling of the fracture void space, arrival times suggest the existence of rapid preferential flow along fractures, fracture networks, and fault zones, even if the matrix is not fully saturated. Modeling such flows requires efficient numerical techniques to cover various flow-relevant physics, such as surface tension, static and dynamic contact angles, free-surface (multi-phase) interface dynamics, and formation of singularities. Here we demonstrate the importance of such flow modes on the partitioning dynamics at simple fracture intersections, with a combination of laboratory experiments, analytical solutions and numerical simulations using our newly developed massively parallel smoothed particle hydrodynamics (SPH) code. Flow modes heavily influence the "bypass" behavior of water flowing along a fracture junction. Flows favoring the formation of droplets exhibit a much stronger bypass capacity compared to rivulet flows, where nearly the whole fluid mass is initially stored within the horizontal fracture. This behavior is demonstrated for a multi-inlet laboratory setup where the inlet-specific flow rate is chosen so that either a droplet or rivulet flow persists. The effect of fluid buffering within the horizontal fracture is presented in terms of dimensionless fracture inflow so that characteristic scaling regimes can be recovered. For both cases (rivulets and droplets), flow within the horizontal fracture transitions into a Washburn regime until a critical threshold is reached and the bypass efficiency increases. For rivulet flows, the initial filling of the horizontal fracture is described by classical plug flow. Meanwhile, for droplet flows, a size-dependent partitioning behavior is observed, and the filling of the fracture takes longer.

Capillary rise in a textured channel

NASA Astrophysics Data System (ADS)

Beilharz, Daniel; Clanet, Christophe; Quere, David

2016-11-01

A wetting liquid can invade a textured material, for example a forest of micropillars. The driving and the viscous forces of this motion are determined by the texture parameters and the influence of shape, height and spacing of posts has been widely studied for the last decade. In this work, we build a channel with textured walls. Brought into contact with a reservoir of wetting liquid, we observe in some cases two advancing fronts. A first one ahead invading the forest of micropillars, and a second one behind filling the remaining gap. We study and model the conditions of existence and the dynamics of these two fronts as a function of the characteristics of both microstructure and gap of this elementary porous medium.

Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal.

PubMed

Li, Chenxi; Wang, Ruikang

2017-04-01

We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes.

Surfactant effects on contact line alteration of a liquid drop in a capillary tube

NASA Astrophysics Data System (ADS)

Yulianti, K.; Marwati, R.

2018-05-01

In this paper, the effect of an insoluble surfactant on the moving contact line of an interface between two fluids filling a capillary tube is studied. The governing equations are the incompressible Navier-Stokes equations with the couple of Eulerian fluid variables and Lagrangian interfacial markers. In our model, capillary force plays a role in the fluids motion. Here, we propose that besides lowering the interfacial tension which affects the capillary force, the surfactant also decreases the surface tension between fluids and a solid surface. That condition is applied to the unbalanced Young condition at the contact line. The front-tracking method is used to solve numerically the free boundary motion of the interface. Results show that the surfactant has a significant effect on the motion of the contact line.

Effect of ultrasonic capillary dynamics on the mechanics of thermosonic ball bonding.

PubMed

Huang, Yan; Shah, Aashish; Mayer, Michael; Zhou, Norman Y; Persic, John

2010-01-01

Microelectronic wire bonding is an essential step in today's microchip production. It is used to weld (bond) microwires to metallized pads of integrated circuits using ultrasound with hundreds of thousands of vibration cycles. Thermosonic ball bonding is the most popular variant of the wire bonding process and frequently investigated using finite element (FE) models that simplify the ultrasonic dynamics of the process with static or quasistatic boundary conditions. In this study, the ultrasonic dynamics of the bonding tool (capillary), made from Al(2)O(3), is included in a FE model. For more accuracy of the FE model, the main material parameters are measured. The density of the capillary was measured to be rho(cap) = 3552 +/- 100 kg/m(3). The elastic modulus of the capillary, E(cap) = 389 +/- 11 GPa, is found by comparing an auxiliary FE model of the free vibrating capillary with measured values. A capillary "nodding effect" is identified and found to be essential when describing the ultrasonic vibration shape. A main FE model builds on these results and adds bonded ball, pad, chip, and die attach components. There is excellent agreement between the main model and the ultrasonic force measured at the interface on a test chip with stress microsensors. Bonded ball and underpad stress results are reported. When adjusted to the same ultrasonic force, a simplified model without ultrasonic dynamics and with an infinitely stiff capillary tip is substantially off target by -40% for the maximum underpad stress. The compliance of the capillary causes a substantial inclination effect at the bonding interface between wire and pad. This oscillating inclination effect massively influences the stress fields under the pad and is studied in more detail. For more accurate results, it is therefore recommended to include ultrasonic dynamics of the bonding tool in mechanical FE models of wire bonding.

Hydraulic droplet coarsening in open-channel capillaries

NASA Astrophysics Data System (ADS)

Warren, Patrick B.

2016-11-01

Over a range of liquid-solid contact angles, an open-channel capillary with curved or angled sides can show a maximum in the Laplace pressure as a function of the filling state. Examples include double-angle wedges, grooves scored into flat surfaces, steps on surfaces, and the groove between touching parallel cylinders. The liquid in such a channel exhibits a beading instability if the channel is filled beyond the Laplace pressure maximum. The subsequent droplet coarsening takes place by hydraulic transport through the connecting liquid columns that remain in the groove. A mean-field scaling argument predicts the characteristic droplet radius R ˜t1 /7 , as a function of time t . This is confirmed by one-dimensional simulations of the coarsening kinetics. Some remarks are also made on the spreading kinetics of an isolated drop deposited in such a channel.

Modelling infiltration processes in frozen soils

NASA Astrophysics Data System (ADS)

Ireson, A. M.; Barbour, L. S.

2014-12-01

Understanding the hydrological processes in soils subject to significant freeze-thaw is fraught by "experimental vagaries and theoretical imponderables" (Miller 1980, Applications of soil physics). The infiltration of snowmelt water and the subsequent transmission of unfrozen water during thawing, is governed by hydraulic conductivity values which are changing with both ice and unfrozen water content. Water held within pores is subject to capillary forces, which results in a freezing point depression (i.e. water remains in the liquid state slightly below 0°C). As the temperature drops below zero, water freezes first in the larger pores, and then in progressively smaller pores. Since the larger pores also are the first to empty by drainage, these pores may be air filled during freezing, while smaller water filled pores freeze. This explains why an unsaturated, frozen soil may still have a considerable infiltration capacity. Infiltration into frozen soil is a critical phenomena related to the risk of flooding in the Canadian prairies, controlling the partitioning of snowmelt into either infiltration or runoff. We propose a new model, based on conceptualizing the pore space as a bundle of capillary tubes (with significant differences to the capillary bundle model of Wannatabe and Flury, 2008, WRR, doi:10.1029/2008WR007102) which allows any air-filled macropores to contribute to the potential infiltration capacity of the soil. The patterns of infiltration and water movement during freeze-thaw from the model are compared to field observations from the Canadian prairies and Boreal Plains.

Reconstruction of thin fluorophore-filled capillaries in thick scattering medium using fluorescence diffuse optical tomography within the diffusion approximation

NASA Astrophysics Data System (ADS)

Desrochers, Johanne; Vermette, Patrick; Fontaine, Réjean; Bérubé-Lauzière, Yves

2009-02-01

Current efforts in tissue engineering target the growth of 3D volumes of tissue cultures in bioreactor conditions. Fluorescence optical tomography has the potential to monitor cells viability and tissue growth non-destructively directly within the bioreactor via bio-molecular fluorescent labelling strategies. We currently work on developing the imaging instrumentation for tissue cultures in bioreactor conditions. Previously, we localized in 3D thin fluorescent-labelled capillaries in a cylindrically shaped bioreactor phantom containing a diffusive medium with our time-of-flight localization technique. Here, we present our first reconstruction results of the spatial distribution of fluorophore concentrations for labelled capillaries embedded in a bioreactor phantom.

Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer

DOEpatents

Burtis, Carl A.; Johnson, Wayne F.; Walker, William A.

1988-01-01

A rotor and disc assembly for use in a centrifugal fast analyzer. The assembly is designed to process multiple samples of whole blood followed by aliquoting of the resultant serum into precisely measured samples for subsequent chemical analysis. The assembly requires minimal operator involvement with no mechanical pipetting. The system comprises (1) a whole blood sample disc, (2) a serum sample disc, (3) a sample preparation rotor, and (4) an analytical rotor. The blood sample disc and serum sample disc are designed with a plurality of precision bore capillary tubes arranged in a spoked array. Samples of blood are loaded into the blood sample disc in capillary tubes filled by capillary action and centrifugally discharged into cavities of the sample preparation rotor where separation of serum and solids is accomplished. The serum is loaded into the capillaries of the serum sample disc by capillary action and subsequently centrifugally expelled into cuvettes of the analytical rotor for analysis by conventional methods.

Optical fiber nanoprobe preparation for near-field optical microscopy by chemical etching under surface tension and capillary action.

PubMed

Mondal, Samir K; Mitra, Anupam; Singh, Nahar; Sarkar, S N; Kapur, Pawan

2009-10-26

We propose a technique of chemical etching for fabrication of near perfect optical fiber nanoprobe (NNP). It uses photosensitive single mode optical fiber to etch in hydro fluoric (HF) acid solution. The difference in etching rate for cladding and photosensitive core in HF acid solution creates capillary ring along core-cladding boundary under a given condition. The capillary ring is filled with acid solution due to surface tension and capillary action. Finally it creates near perfect symmetric tip at the apex of the fiber as the height of the acid level in capillary ring decreases while width of the ring increases with continuous etching. Typical tip features are short taper length (approximately 4 microm), large cone angle (approximately 38 degrees ), and small probe tip dimension (<100 nm). A finite difference time domain (FDTD) analysis is also presented to compare near field optics of the NNP with conventional nanoprobe (CNP). The probe may be ideal for near field optical imaging and sensor applications.

Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal

PubMed Central

Li, Chenxi; Wang, Ruikang

2017-01-01

Abstract. We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes. PMID:28384709

A Zero Dimensional Time-Dependent Model of High-Pressure Ablative Capillary Discharge (Preprint)

DTIC Science & Technology

2008-06-01

comprehensive model of capillary discharge is important to understand the physics and engineering aspects of the capillary discharge thruster. A schematic...investigators since the mid-1980s see 1-11 and references therein, satisfy both of these conditions well. These studies investigated the dynamics of high...is a comprehensive description of the radiative heat transfer in the capillary discharge. It is worth noting that in other types of capillary

Electron density measurement in gas discharge plasmas by optical and acoustic methods

NASA Astrophysics Data System (ADS)

Biagioni, A.; Anania, M. P.; Bellaveglia, M.; Chiadroni, E.; Cianchi, A.; Di Giovenale, D.; Di Pirro, G.; Ferrario, M.; Filippi, F.; Mostacci, A.; Pompili, R.; Shpakov, V.; Vaccarezza, C.; Villa, F.; Zigler, A.

2016-08-01

Plasma density represents a very important parameter for both laser wakefield and plasma wakefield acceleration, which use a gas-filled capillary plasma source. Several techniques can be used to measure the plasma density within a capillary discharge, which are mainly based on optical diagnostic methods, as for example the well-known spectroscopic method using the Stark broadening effect. In this work, we introduce a preliminary study on an alternative way to detect the plasma density, based on the shock waves produced by gas discharge in a capillary. Firstly, the measurements of the acoustic spectral content relative to the laser-induced plasmas by a solid target allowed us to understand the main properties of the acoustic waves produced during this kind of plasma generation; afterwards, we have extended such acoustic technique to the capillary plasma source in order to calibrate it by comparison with the stark broadening method.

Intermittent KoldBlue cryotherapy of 3×10 min changes mid‐portion Achilles tendon microcirculation

PubMed Central

Knobloch, Karsten; Grasemann, Ruth; Spies, Marcus; Vogt, Peter M

2007-01-01

Background Neovascularisation and microcirculatory changes have been reported in Achilles tendinopathy. Cryotherapy and compression, as part of a rest, ice, compression and elevation regimen, are shown to decrease pain and improve function. However, the microcirculatory changes following a given dosage of cryotherapy on mid‐portion Achilles tendon remain unclear. Study design Prospective clinical cohort study, level of evidence 2. Methods 30 people (12 males, 33 (SD 12) years, body mass index 25.6 (5.3) kg/m2) were included in the cohort. 3×10 min KoldBlue ankle‐cooling bandages were applied and microcirculation of Achilles tendon mid‐portion was real‐time and continuously assessed using a laser‐Doppler‐spectrophotometry system (O2C, Germany). Results Superficial capillary blood flow was reduced from 42 to 6, 5 and 3 relative units (rU) in the first, second and third cryotherapy periods, respectively (−65%, p = 0.001), with no significant capillary hyperaemia. Deep capillary tendon blood flow was reduced from 180 to 82, 53 and 52 rU (−71%, p = 0.001) within 6–9 min of application without hyperaemia. Superficial tendon oxygen saturation dropped significantly from 43% to 26%, 18% and 11% (p = 0.001) after repetitive cryotherapy, with persisting increase of tendon oxygenation during rewarming (51%, 49% and 54%, p = 0.077) up to 27% of the baseline level. At 8 mm tendon depth, cryotherapy preserved local oxygenation. Relative postcapillary venous tendon filling pressures were favourably reduced from 41 (11) to 31, 28 and 26 rU (−36%, p = 0.001) superficially and from 56 (11) to 45, 46 and 48 rU (−18%, p = 0.001) in deep capillary blood flow during cryotherapy, facilitating capillary venous clearance. Conclusion Intermittent cryotherapy of 3×10 min significantly decreases local Achilles tendon mid‐portion capillary blood flow by 71%. Within 2 min of rewarming, tendon oxygen saturation is re‐established following cryotherapy. Postcapillary venous filling pressures are reduced during cryotherapy, favouring capillary venous outflow of the healthy Achilles tendon. PMID:17138636

Three-dimensional spatial localization of thin fluorophore-filled capillaries in thick scattering media

NASA Astrophysics Data System (ADS)

Desrochers, Johanne; Vermette, Patrick; Fontaine, Réjean; Bérubé-Lauzière, Yves

2008-06-01

Fluorescence optical diffuse tomography (fDOT) is of much interest in molecular imaging to retrieve information from fluorescence signals emitted from specifically targeted bioprocesses deep within living tissues. An exciting application of fDOT is in the growing field of tissue engineering, where 3D non-invasive imaging techniques are required to ultimately grow 3D engineered tissues. Via appropriate labelling strategies and fluorescent probes, fDOT has the potential to monitor culture environment and cells viability non-destructively directly within the bioreactor environment where tissues are to be grown. Our ultimate objective is to image the formation of blood vessels in bioreactor conditions. Herein, we use a non-contact setup for small animal fDOT imaging designed for 3D light collection around the sample. We previously presented a time of flight approach using a numerical constant fraction discrimination technique to assign an early photons arrival time to every fluorescence time point-spread function collected around the sample. Towards bioreactor in-situ imaging, we have shown the capability of our approach to localize a fluorophore-filled 500 μm capillary immersed coaxially in a cylindrically shaped bioreactor phantom containing an absorbing/scattering medium representative of experiments on real tissue cultures. Here, we go one step further, and present results for the 3D localization of thinner indocyanine green labelled capillaries (250 μm and 360 μm inner diameter) immersed in the same phantom conditions and geometry but with different spatial configurations (10° and 30° capillary inclination).

Instability of the capillary bridge

NASA Astrophysics Data System (ADS)

Pare, Gounseti; Hoepffner, Jerome

2014-11-01

Capillary adhesion is a physical mechanism that maintains two bodies in contact by capillarity through a liquid ligament. The capillary bridge is an idealization of this capillary adhesion. In this study we first focus on the classical case of the stability of the capillary bridge. Secondly we study a slightly more complex configuration, imagining a flow in the capillary bridge as in the case of the dynamics of the neck of a liquid ligament, in its withdrawal under the effect of capillarity. Inspired by the experiments on soap films of Plateau, the configuration analyzed consists of an initially axisymmetric, mass of fluid held by surface tension forces between two parallel, coaxial, solid pipes of the same diameter. The results presented are obtained by numerical simulations using the free software, Gerris Flow Solver. We first focus on the capillary Venturi. In the static configuration the stability diagram of the capillary bridge obtained is in perfect agreement with the results of Lev A. Slobozhanin. In the dynamic case we develop a matlab code based on the one dimensional equations of Eggers and Dupont. The comparison of the bifurcation diagram obtained and the numerical simulations shows a good agreement.

A comparison of capillary and rotational viscometry of aqueous solutions of hypromellose.

PubMed

Sklubalová, Z; Zatloukal, Z

2007-10-01

A comparison of capillary and rotational viscometry of gentle pseudoplastic solutions of hypromellose (HPMC 4000) by using only single-point value of viscosity is difficult. Single-point comparison becomes topical in consequence to the pharmacopoeial requirement that the apparent viscosity of 2% hypromellose solution should be read at the shear rate of approximately 10 s(-1). This communication is focused on the estimation of the suitable shear rate, D eta, at which the apparent viscosity read using the rotational viscometer is numerically equal to the dynamic viscosity read using a capillary viscometer. For the solutions of HPMC in concentrations up to 2% w/v, the non-linear regression equations generated showed the influencing of the D eta value by the dynamic viscosity and/or by the originally derived linear velocity of the solution flowing through the capillary viscometer tube. To compare the apparent viscosity read using the rotational viscometer with the dynamic viscosity read using capillary viscometer, the exact estimation of the shear rate D eta at which both viscosities are numerically equal is essential since it is markedly affected by the concentration of HPMC solution.

Direct Numerical Simulations of Dynamic Drainage and Imbibition to Investigate Capillary Pressure-Saturation-Interfacial Area Relation

NASA Astrophysics Data System (ADS)

Konangi, S.; Palakurthi, N. K.; Karadimitriou, N.; Comer, K.; Ghia, U.

2017-12-01

We present results of pore-scale direct numerical simulations (DNS) of drainage and imbibition in a quasi-two-dimensional (40µm thickness) porous medium with a randomly distributed packing of cylindrical obstructions. The Navier-Stokes (NS) equations are solved in the pore space on an Eulerian mesh using the open-source finite-volume computational fluid dynamics (CFD) code, OpenFOAM. The Volume-of-Fluid (VOF) method is employed to track the evolution of the fluid-fluid interface; a static contact angle is used to account for wall adhesion. From the DNS data, we focus on the macroscopic capillary pressure-saturation (Pc-Sw) relation, which is known to be hysteretic, i.e., this relation is flow process (such as drainage, imbibition and scanning curves) and history dependent. In order to overcome the problem of hysteresis, extended theories of multiphase flow hypothesized that the inclusion of specific interfacial area as a state variable will result in a unique relation between capillary pressure, saturation and interfacial area (Pc-Sw-awn). We study the role of specific interfacial area on hysteresis in the macroscopic Pc-Sw relation under non-equilibrium (dynamic) conditions. Under dynamic conditions, capillary pressure depends on the rate of change of the wetting phase saturation, and the dynamic Pc-Sw relation includes the changes caused by viscous effects. Simulations of drainage and imbibition are performed for two capillary numbers by controlling the flow rate of the non-wetting (polydimenthlysiloxane oil) and wetting (water) fluids. From these simulations, the Pc-Sw curves will be estimated; the Pc-S-awn surface will be constructed to determine whether the data points from drainage and imbibition processes fall on a unique surface under transient conditions. Different macroscopic capillary pressure definitions based on phase-averaged pressures and interfacial area will be evaluated. Understanding macroscopic capillary pressure definitions and the uniqueness of the Pc-S- awn relation is step towards complete description of two-phase flow at the Darcy scale.

A Galinstan-Filled Capillary Probe for Thermal Conductivity Measurements and Its Application to Molten Eutectic {KNO}_3-{NaNO}_3-{NO}_2 (HTS) up to 700 K

NASA Astrophysics Data System (ADS)

Le Brun, Niccolò; Markides, Christos N.

2015-11-01

The successful measurement of the thermal conductivity of molten salts is a challenging undertaking due to the electrically conducting and possibly also aggressive nature of the materials, as well as the elevated temperatures at which these data are required. For accurate and reproducible measurements, it is important to develop a suitable experimental apparatus and methodology. In this study, we explore a modified version of the transient hot-wire method, which employs a molten-metal-filled capillary in order to circumvent some of the issues encountered in previous studies. Specifically, by using a novel flexible U-shaped quartz-capillary, filled with a eutectic mixture of gallium, indium and tin, commercially known as Galinstan, we proceed to measure the thermal conductivity of molten eutectic {KNO}_3-{NaNO}_3-{NaNO}_2. The new probe is demonstrated as being able to measure the thermal conductivity of this molten salt, which is found to range from 0.48 {W}{\\cdot }{m}^{-1}{\\cdot }{K}^{-1} at 500 K to 0.47 {W}{\\cdot }{m}^{-1}{\\cdot }{K}^{-1} at close to 700 K, with an overall expanded uncertainty (95 % confidence) of 3.1 %. The quartz is found to retain its electrically insulating properties and no current leakage is detected in the sample over the investigated temperature range. The thermal conductivity data reported in the present study are also used to elucidate a partial disagreement found in the literature for this material.

Capillary-tube-based extension of thermoacoustic theory for a random medium

NASA Astrophysics Data System (ADS)

Roh, Heui-Seol; Raspet, Richard; Bass, Henry E.

2005-09-01

Thermoacoustic theory for a single capillary tube is extended to random bulk medium on the basis of capillary tubes. The characteristics of the porous stack inside the resonator such as the tortuosity, dynamic shape factor, and porosity are introduced for the extension of wave equation by following Attenborough's approach. Separation of the dynamic shape factor for the viscous and thermal effect is adopted and scaling using the dynamic shape factor and tortuosity factor is demonstrated. The theoretical and experimental comparison of thermoviscous functions in reticulated vitreous carbon (RVC) and aluminum foam shows reasonable agreement. The extension is useful for investigations of the properties of a stack with arbitrary shapes of non-parallel pores.

Sloshing dynamics on rotating helium dewar tank

NASA Technical Reports Server (NTRS)

Hung, R. J.

1993-01-01

The generalized mathematical formulation of sloshing dynamics for partially filled liquid of cryogenic superfluid helium II in dewar containers driven by both the gravity gradient and jitter accelerations applicable to scientific spacecraft which is eligible to carry out spinning motion and/or slew motion for the purpose to perform scientific observation during the normal spacecraft operation are investigated. An example is given with Gravity Probe-B (GP-B) spacecraft which is responsible for the sloshing dynamics. The jitter accelerations include slew motion, spinning motion, atmospheric drag on the spacecraft, spacecraft attitude motions arising from machinery vibrations, thruster firing, pointing control of spacecraft, crew motion, etc. Explicit mathematical expressions to cover these forces acting on the spacecraft fluid systems are derived. The numerical computation of sloshing dynamics were based on the non-inertia frame spacecraft bound coordinate, and solve time dependent, three-dimensional formulations of partial differential equations subject to initial and boundary conditions. The explicit mathematical expressions of boundary conditions to cover capillary force effect on the liquid vapor interface in microgravity environments are also derived. The formulations of fluid moment and angular moment fluctuations in fluid profiles induced by the sloshing dynamics, together with fluid stress and moment fluctuations exerted on the spacecraft dewar containers were derived. Results were widely published in the open journals.

Capillary-induced crack healing between surfaces of nanoscale roughness.

PubMed

Soylemez, Emrecan; de Boer, Maarten P

2014-10-07

Capillary forces are important in nature (granular materials, insect locomotion) and in technology (disk drives, adhesion). Although well studied in equilibrium state, the dynamics of capillary formation merit further investigation. Here, we show that microcantilever crack healing experiments are a viable experimental technique for investigating the influence of capillary nucleation on crack healing between rough surfaces. The average crack healing velocity, v̅, between clean hydrophilic polycrystalline silicon surfaces of nanoscale roughness is measured. A plot of v̅ versus energy release rate, G, reveals log-linear behavior, while the slope |d[log(v̅)]/dG| decreases with increasing relative humidity. A simplified interface model that accounts for the nucleation time of water bridges by an activated process is developed to gain insight into the crack healing trends. This methodology enables us to gain insight into capillary bridge dynamics, with a goal of attaining a predictive capability for this important microelectromechanical systems (MEMS) reliability failure mechanism.

Dynamics and density distributions in a capillary-discharge waveguide with an embedded supersonic jet

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matlis, N. H., E-mail: nmatlis@gmail.com; Gonsalves, A. J.; Steinke, S.

We present an analysis of the gas dynamics and density distributions within a capillary-discharge waveguide with an embedded supersonic jet. This device provides a target for a laser plasma accelerator which uses longitudinal structuring of the gas-density profile to enable control of electron trapping and acceleration. The functionality of the device depends sensitively on the details of the density profile, which are determined by the interaction between the pulsed gas in the jet and the continuously-flowing gas in the capillary. These dynamics are captured by spatially resolving recombination light from several emission lines of the plasma as a function ofmore » the delay between the jet and the discharge. We provide a phenomenological description of the gas dynamics as well as a quantitative evaluation of the density evolution. In particular, we show that the pressure difference between the jet and the capillary defines three regimes of operation with qualitatively different longitudinal density profiles and show that jet timing provides a sensitive method for tuning between these regimes.« less

Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo

NASA Astrophysics Data System (ADS)

Yao, Junjie; Maslov, Konstantin; Hu, Song; Wang, Lihong V.

2009-09-01

Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we use Evans blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption and distributes uniformly in the blood stream by chemically binding to albumin. With the help of EB, complete and continuous microvascular networks--especially capillaries--are imaged. The diffusion dynamics of EB leaving the blood stream and the clearance dynamics of the EB-albumin complex are also quantitatively investigated.

Liquid Acquisition Device Design Sensitivity Study

NASA Technical Reports Server (NTRS)

VanDyke, M. K.; Hastings, L. J.

2012-01-01

In-space propulsion often necessitates the use of a capillary liquid acquisition device (LAD) to assure that gas-free liquid propellant is available to support engine restarts in microgravity. If a capillary screen-channel device is chosen, then the designer must determine the appropriate combination screen mesh and channel geometry. A screen mesh selection which results in the smallest LAD width when compared to any other screen candidate (for a constant length) is desirable; however, no best screen exists for all LAD design requirements. Flow rate, percent fill, and acceleration are the most influential drivers for determining screen widths. Increased flow rates and reduced percent fills increase the through-the-screen flow pressure losses, which drive the LAD to increased widths regardless of screen choice. Similarly, increased acceleration levels and corresponding liquid head pressures drive the screen mesh selection toward a higher bubble point (liquid retention capability). After ruling out some screens on the basis of acceleration requirements alone, candidates can be identified by examining screens with small flow-loss-to-bubble point ratios for a given condition (i.e., comparing screens at certain flow rates and fill levels). Within the same flow rate and fill level, the screen constants inertia resistance coefficient, void fraction, screen pore or opening diameter, and bubble point can become the driving forces in identifying the smaller flow-loss-to-bubble point ratios.

Investigation of Coronal Leakage of Root Fillings after Smear Layer Removal with EDTA or Er,Cr:YSGG Laser through Capillary Flow Porometry

PubMed Central

Vergauwen, Tom Edgard Maria; Michiels, Rafaël; Torbeyns, Dries; Meire, Maarten; De Moor, Roeland Jozef Gentil

2014-01-01

No studies have been performed evaluating the marginal seal of root fillings after direct exposure of root canal (RC) walls to Er,Cr:YSGG laser irradiation. Therefore, 75 root filled teeth (5 × 15–cold lateral condensation) were analyzed for through-and-through leakage (TTL) using capillary flow porometry (CFP). The cleaning protocol determined the experimental groups: (1) irrigation with NaOCl 2.5% and EDTA 17% or standard protocol (SP), (2) SP + Er,Cr:YSGG lasing (dried RC), (3) NaOCl 2.5% + Er,Cr:YSGG lasing (dried RC), (4) SP + Er,Cr:YSGG lasing (wet RC), and (5) NaOCl 2.5% + Er,Cr:YSGG lasing (wet RC). Groups 6 to 10 consisted of the same filled teeth with resected apices. Resection was performed after the first CFP measurement. CFP was used to assess minimum, mean flow, and maximum pore diameters after 48 h. Statistics were performed using nonparametric tests (P > 0.05). Additional three roots per group were submitted to SEM of the RC walls. TTL was observed in all groups without statistically significant differences between the different groups for minimum, mean, and maximum pore diameter (P > 0.05). In this study, the use of EDTA and/or Er,Cr:YSGG laser did not reduce through-and-through leakage in nonresected and resected roots. PMID:24696685

Effective slip over partially filled microcavities and its possible failure

NASA Astrophysics Data System (ADS)

Ge, Zhouyang; Holmgren, Hanna; Kronbichler, Martin; Brandt, Luca; Kreiss, Gunilla

2018-05-01

Motivated by the emerging applications of liquid-infused surfaces (LIS), we study the drag reduction and robustness of transverse flows over two-dimensional microcavities partially filled with an oily lubricant. Using separate simulations at different scales, characteristic contact line velocities at the fluid-solid intersection are first extracted from nanoscale phase field simulations and then applied to micronscale two-phase flows, thus introducing a multiscale numerical framework to model the interface displacement and deformation within the cavities. As we explore the various effects of the lubricant-to-outer-fluid viscosity ratio μ˜2/μ˜1 , the capillary number Ca, the static contact angle θs, and the filling fraction of the cavity δ , we find that the effective slip is most sensitive to the parameter δ . The effects of μ˜2/μ˜1 and θs are generally intertwined but weakened if δ <1 . Moreover, for an initial filling fraction δ =0.94 , our results show that the effective slip is nearly independent of the capillary number when it is small. Further increasing Ca to about 0.01 μ˜1/μ˜2 , we identify a possible failure mode, associated with lubricants draining from the LIS, for μ˜2/μ˜1≲0.1 . Very viscous lubricants (e.g., μ˜2/μ˜1>1 ), however, are immune to such failure due to their generally larger contact line velocity.

Capillary red blood cell velocimetry by phase-resolved optical coherence tomography

NASA Astrophysics Data System (ADS)

Tang, Jianbo; Erdener, Sefik Evren; Fu, Buyin; Boas, David A.

2018-02-01

Quantitative measurement of blood flow velocity in capillaries is challenging due to their small size (around 5-10 μm), and the discontinuity and single-file feature of RBCs flowing in a capillary. In this work, we present a phase-resolved Optical Coherence Tomography (OCT) method for accurate measurement of the red blood cell (RBC) speed in cerebral capillaries. To account for the discontinuity of RBCs flowing in capillaries, we applied an M-mode scanning strategy that repeated A-scans at each scanning position for an extended time. As the capillary size is comparable to the OCT resolution size (3.5×3.5×3.5μm), we applied a high pass filter to remove the stationary signal component so that the phase information of the dynamic component (i.e. from the moving RBC) could be enhanced to provide an accurate estimate of the RBC axial speed. The phase-resolved OCT method accurately quantifies the axial velocity of RBC's from the phase shift of the dynamic component of the signal. We validated our measurements by RBC passage velocimetry using the signal magnitude of the same OCT time series data. These proposed method of capillary velocimetry proved to be a robust method of mapping capillary RBC speeds across the micro-vascular network.

Primary drainage in geological fractures: Effects of aperture variability and wettability

NASA Astrophysics Data System (ADS)

Yang, Z.; Méheust, Y.; Neuweiler, I.

2017-12-01

Understanding and controlling fluid-fluid displacement in porous and fractured media is a key asset for many practical applications, such as the geological storage of CO2, hydrocarbon recovery, groundwater remediation, etc. We numerically investigate fluid-fluid displacement in rough-walled fractures with a focus on the combined effect of wettability, the viscous contrast between the two fluids, and fracture surface topography on drainage patterns and interface growth. A model has been developed to simulate the dynamic displacement of one fluid by another immiscible one in a rough geological fracture; the model takes both capillary and viscous forces into account. Capillary pressures at the fluid-fluid interface are calculated based on the Young-Laplace equation using the two principal curvatures (aperture-induced curvature and in-plane curvature) [1], while viscous forces are calculated by continuously solving the fluid pressure field in the fracture. The aperture field of a fracture is represented by a spatially correlated random field, with a power spectral density of the fracture wall topographies scaling as a power law, and a cutoff wave-length above which the Fourier modes of the two walls are identical [2]. We consider flow scenarios with both rectangular and radial configurations. Results show that the model is able to produce displacement patterns of compact displacement, capillary fingering, and viscous fingering, as well as the transitions between them. Both reducing the aperture variability and increasing the contact angle (from drainage to weak imbibition) can stabilize the displacement due to the influence of the in-plane curvature, an effect analogous to that of the cooperative pore filling in porous media. These results suggest that for geometries typical of geological fractures we can extend the phase diagram in the parameter space of capillary number and mobility ratio by another dimension to take into account the combined effect of wettability and fracture aperture topography. References: [1] Yang, Z. et al. (2012), A generalized approach for estimation of in-plane curvature in invasion percolation models for drainage in fractures. Wat. Resour. Res., 48(9), W09507. [2] Yang, Z. et al. (2016), Fluid trapping during capillary displacement in fractures. Adv. Water Resour., 95, 264-275.

Drainage and impregnation capillary pressure curves calculated by the X-ray CT model of Berea sandstone using Lattice Boltzmann's method

NASA Astrophysics Data System (ADS)

Zakirov, T.; Galeev, A.; Khramchenkov, M.

2018-05-01

The study deals with the features of the technique for simulating the capillary pressure curves of porous media on their X-ray microtomographic images. The results of a computational experiment on the immiscible displacement of an incompressible fluid by another in the pore space represented by a digital image of the Berea sandstone are presented. For the mathematical description of two-phase fluid flow we use Lattice Boltzmann Equation (LBM), and phenomena at the fluids interface are described by the color-gradient model. Compared with laboratory studies, the evaluation of capillary pressure based on the results of a computational filtration experiment is a non-destructive method and has a number of advantages: the absence of labor for preparation of fluids and core; the possibility of modeling on the scale of very small core fragments (several mm), which is difficult to realize under experimental conditions; three-dimensional visualization of the dynamics of filling the pore space with a displacing fluid during drainage and impregnation; the possibility of carrying out multivariate calculations for specified parameters of multiphase flow (density and viscosity of fluids, surface tension, wetting contact angle). A satisfactory agreement of the capillary pressure curves during drainage with experimental results was obtained. It is revealed that with the increase in the volume of the digital image, the relative deviation of the calculated and laboratory data decreases and for cubic digital cores larger than 1 mm it does not exceed 5%. The behavior of the non-wetting fluid flow during drainage is illustrated. It is shown that flow regimes under which computational and laboratory experiments are performed the distribution of the injected phase in directions different from the gradient of the hydrodynamic drop, including the opposite ones, is characteristic. Experimentally confirmed regularities are obtained when carrying out calculations for drainage and imbibition at different values of interfacial tension. There is a close coincidence in the average diameters of permeable channels, estimated by capillary curves for different interfacial tension and pore network model. The differences do not exceed 15%.

Impact of eliminating fracture intersection nodes in multiphase compositional flow simulation

NASA Astrophysics Data System (ADS)

Walton, Kenneth M.; Unger, Andre J. A.; Ioannidis, Marios A.; Parker, Beth L.

2017-04-01

Algebraic elimination of nodes at discrete fracture intersections via the star-delta technique has proven to be a valuable tool for making multiphase numerical simulations more tractable and efficient. This study examines the assumptions of the star-delta technique and exposes its effects in a 3-D, multiphase context for advective and dispersive/diffusive fluxes. Key issues of relative permeability-saturation-capillary pressure (kr-S-Pc) and capillary barriers at fracture-fracture intersections are discussed. This study uses a multiphase compositional, finite difference numerical model in discrete fracture network (DFN) and discrete fracture-matrix (DFM) modes. It verifies that the numerical model replicates analytical solutions and performs adequately in convergence exercises (conservative and decaying tracer, one and two-phase flow, DFM and DFN domains). The study culminates in simulations of a two-phase laboratory experiment in which a fluid invades a simple fracture intersection. The experiment and simulations evoke different invading fluid flow paths by varying fracture apertures as oil invades water-filled fractures and as water invades air-filled fractures. Results indicate that the node elimination technique as implemented in numerical model correctly reproduces the long-term flow path of the invading fluid, but that short-term temporal effects of the capillary traps and barriers arising from the intersection node are lost.

Influence of granulometry in the Hurst exponent of air liquid interfaces formed during capillary rising in a granular media

NASA Astrophysics Data System (ADS)

Gontijo, Guilherme L.; Souza, Flávia B.; Braga, Rafael M. L.; Silva, Pedro H. E.; Correia, Maury D.; Atman, A. P. F.

2017-06-01

We report results concerning the fractal dimension of a air/fluid interface formed during the capillary rising of a fluid into a dense granular media. The system consists in a modified Hele-Shaw cell filled with grains at different granulometries and confined in a narrow gap between the glass plates. The system is then placed onto a water reservoir, and the liquid penetrates the medium due to capillary forces. We measure the Hurst exponent of the liquid/air interface with help of image processing, and follow the temporal evolution of the profiles. We observe that the Hurst exponent can be related with the granulometry, but the range of values are odd to the predicted values from models or theory.

Properties of pendular liquid bridges determined on Delaunay's roulettes

NASA Astrophysics Data System (ADS)

Mielniczuk, Boleslaw; Millet, Olivier; Gagneux, Gérard; El Youssoufi, Moulay Said

2017-06-01

This work addresses the study of capillary bridge properties between two grains, with use of recent analytical model, based on solutions of Young-Laplace equation from an inverse problem. A simple explicit criterion allows to classify the profile of capillary bridge as a surface of revolution with constant mean curvature (Delaunay roulette) using its measured geometrical parameters (gorge radius, contact angle, half-filling angle). Necessary data are obtained from experimental tests, realized on liquid bridges between two equal spherical grains. Sequences of images are recorded at several (fixed) volumes of liquid and different separations distances between the spheres (from contact to rupture), in laboratory and in micro-gravity conditions. For each configuration, an exact parametric representation of the meridian is revealed. Mean bridge curvature, internal pressure and intergranular capillary force are also determined.

Proof Of Concept of Integrated Load Measurement in 3D Printed Structures

DOE PAGES

Hinderdael, Michael; Strantza, Maria; De Baere, Dieter; ...

2017-02-09

Currently, research on structural health monitoring systems is focused on direct integration of the system into a component or structure. The latter results in a so-called smart structure. One example of a smart structure is a component with integrated strain sensing for continuous load monitoring. Additive manufacturing, or 3D printing, now also enables such integration of functions inside components. As a proof-of-concept, the Fused Deposition Modeling (FDM) technique was used to integrate a strain sensing element inside polymer (ABS) tensile test samples. The strain sensing element consisted of a closed capillary filled with a fluid and connected to an externallymore » mounted pressure sensor. The volumetric deformation of the integrated capillary resulted in pressure changes in the fluid. The obtained pressure measurements during tensile testing are reported in this paper and compared to state-of-the-art extensometer measurements. The sensitivity of the 3D printed pressure-based strain sensor is primarily a function of the compressibility of the capillary fluid. Air- and watertightness are of critical importance for the proper functioning of the 3D printed pressure-based strain sensor. Therefore, the best after-treatment procedure was selected on basis of a comparative analysis. The obtained pressure measurements are linear with respect to the extensometer readings, and the uncertainty on the strain measurement of a capillary filled with water (incompressible fluid) is ±3.1 µstrain, which is approximately three times less sensitive than conventional strain gauges (±1 µstrain), but 32 times more sensitive than the same sensor based on air (compressible fluid) (±101 µstrain).« less

Proof Of Concept of Integrated Load Measurement in 3D Printed Structures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hinderdael, Michael; Strantza, Maria; De Baere, Dieter

Currently, research on structural health monitoring systems is focused on direct integration of the system into a component or structure. The latter results in a so-called smart structure. One example of a smart structure is a component with integrated strain sensing for continuous load monitoring. Additive manufacturing, or 3D printing, now also enables such integration of functions inside components. As a proof-of-concept, the Fused Deposition Modeling (FDM) technique was used to integrate a strain sensing element inside polymer (ABS) tensile test samples. The strain sensing element consisted of a closed capillary filled with a fluid and connected to an externallymore » mounted pressure sensor. The volumetric deformation of the integrated capillary resulted in pressure changes in the fluid. The obtained pressure measurements during tensile testing are reported in this paper and compared to state-of-the-art extensometer measurements. The sensitivity of the 3D printed pressure-based strain sensor is primarily a function of the compressibility of the capillary fluid. Air- and watertightness are of critical importance for the proper functioning of the 3D printed pressure-based strain sensor. Therefore, the best after-treatment procedure was selected on basis of a comparative analysis. The obtained pressure measurements are linear with respect to the extensometer readings, and the uncertainty on the strain measurement of a capillary filled with water (incompressible fluid) is ±3.1 µstrain, which is approximately three times less sensitive than conventional strain gauges (±1 µstrain), but 32 times more sensitive than the same sensor based on air (compressible fluid) (±101 µstrain).« less

Genotyping of alpha-thalassemia deletions using multiplex polymerase chain reactions and gold nanoparticle-filled capillary electrophoresis.

PubMed

Chen, Yen-Ling; Shih, Chi-Jen; Ferrance, Jerome; Chang, Ya-Sian; Chang, Jan-Gowth; Wu, Shou-Mei

2009-02-13

A gold nanoparticle-filled capillary electrophoresis method combined with three multiplex polymerase chain reactions (PCRs) was established for simultaneous diagnosis of five common alpha-thalassemia deletions, including the -alpha(3.7) deletion, -alpha(4.2) deletion, Southeast Asian (--(SEA)), Filipino (--(FIL)) and Thai (--(THAI)) deletions. Gold nanoparticles (GNPs) were used as a pseudostationary phase to improve the resolution between DNA fragments in a low-viscosity polymer. To achieve the best CE separation, several parameters were evaluated for optimizing the separation conditions, including the capillary coating, the concentrations of polymer sieving matrix, the sizes and concentrations of GNPs, the buffer concentrations, and the pH. The final CE method for separating a 200-base pair (bp) DNA ladder and alpha-thalassemia deletions used a DB-17 capillary, 0.6% poly(ethylene oxide) (PEO) prepared in a mixture of GNP(32nm) solution and glycine buffer (25mM, pH 9.0) (80:20, v/v) as the sieving matrix with 1microM YO-PRO-1 for fluorescence detection; the applied voltage was -10kV (detector at anode side) and the separation temperature was 25 degrees C. Under these optimal conditions, 15 DNA fragments with sizes ranging from 0.2kb to 3.0kb were resolved within 11.5min. The RSDs of migration times were less than 2.81%. A total of 21 patients with alpha-thalassemia deletions were analyzed using this method, and all results showed good agreement with those obtained by gel electrophoresis.

Proof of Concept of Integrated Load Measurement in 3D Printed Structures

PubMed Central

Hinderdael, Michaël; Jardon, Zoé; Lison, Margot; De Baere, Dieter; Devesse, Wim; Strantza, Maria; Guillaume, Patrick

2017-01-01

Currently, research on structural health monitoring systems is focused on direct integration of the system into a component or structure. The latter results in a so-called smart structure. One example of a smart structure is a component with integrated strain sensing for continuous load monitoring. Additive manufacturing, or 3D printing, now also enables such integration of functions inside components. As a proof-of-concept, the Fused Deposition Modeling (FDM) technique was used to integrate a strain sensing element inside polymer (ABS) tensile test samples. The strain sensing element consisted of a closed capillary filled with a fluid and connected to an externally mounted pressure sensor. The volumetric deformation of the integrated capillary resulted in pressure changes in the fluid. The obtained pressure measurements during tensile testing are reported in this paper and compared to state-of-the-art extensometer measurements. The sensitivity of the 3D printed pressure-based strain sensor is primarily a function of the compressibility of the capillary fluid. Air- and watertightness are of critical importance for the proper functioning of the 3D printed pressure-based strain sensor. Therefore, the best after-treatment procedure was selected on basis of a comparative analysis. The obtained pressure measurements are linear with respect to the extensometer readings, and the uncertainty on the strain measurement of a capillary filled with water (incompressible fluid) is ±3.1 µstrain, which is approximately three times less sensitive than conventional strain gauges (±1 µstrain), but 32 times more sensitive than the same sensor based on air (compressible fluid) (±101 µstrain). PMID:28208779

Numerical investigations of two-phase flow with dynamic capillary pressure in porous media via a moving mesh method

NASA Astrophysics Data System (ADS)

Zhang, Hong; Zegeling, Paul Andries

2017-09-01

Motivated by observations of saturation overshoot, this paper investigates numerical modeling of two-phase flow in porous media incorporating dynamic capillary pressure. The effects of the dynamic capillary coefficient, the infiltrating flux rate and the initial and boundary values are systematically studied using a traveling wave ansatz and efficient numerical methods. The traveling wave solutions may exhibit monotonic, non-monotonic or plateau-shaped behavior. Special attention is paid to the non-monotonic profiles. The traveling wave results are confirmed by numerically solving the partial differential equation using an accurate adaptive moving mesh solver. Comparisons between the computed solutions using the Brooks-Corey model and the laboratory measurements of saturation overshoot verify the effectiveness of our approach.

Capillary spreading of contact line over a sinking sphere

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Seong Jin; Fezzaa, Kamel; An, Jim

The contact line dynamics over a sinking solid sphere are investigated in comparison with classical spreading theories. Experimentally, high-speed imaging systems with optical light or x-ray illumination are employed to accurately measure the spreading motion and dynamic contact angle of the contact line. Millimetric spheres are controlled to descend with a constant speed ranging from 7.3 × 10-5 to 0.79 m/s. We observed three different spreading stages over a sinking sphere, which depends on the contact line velocity and contact angle. These stages consistently showed the characteristics of capillarity-driven spreading as the contact line spreads faster with a higher contactmore » angle. The contact line velocity is observed to follow a classical capillary-viscous model at a high Ohnesorge number (> 0.02). For the cases with a relatively low Ohnesorge number (< 0.02), the contact line velocity is significantly lower than the speed predicted by the capillary-viscous balance. This indicates the existence of an additional opposing force (inertia) for a decreasing Ohnesorge number. The capillary-inertial balance is only observed at the very beginning of the capillary rise, in which the maximum velocity is independent of the sphere’s sinking speed. Additionally, we observed the linear relation between the contact line velocity and the sphere sinking speed during the second stage, which represents capillary adjustment by dynamic contact angle.« less

Water infiltration in prewetted porous media: dynamic capillary pressure and Green-Ampt modeling

NASA Astrophysics Data System (ADS)

Hsu, S.; Hilpert, M.

2013-12-01

Recently, an experimental study has shown that the modified Green-Ampt (GA) model, which accounts for a velocity-dependent capillary pressure, can describe water infiltration in dry sand columns better than the classical GA model. Studies have also shown that the initial water content of prewetted porous media affects the dynamic capillary pressure during infiltration. In this study, we performed a series of downward water infiltration experiments in prewetted sand columns for four different initial water contents: 0%, 3.3%, 6.5%, and 13.8%. We also used three different ponding heights: 10 cm, 20 cm, and 40 cm. As expected, an increase in ponding height resulted in a monotonic increase in cumulative infiltration. However, we found anomalous behavior, in that the cumulative infiltration did not monotonically decrease as the initial water content increased. When modeling the experiments with the modified GA approach, we linked this anomalous behavior to the reduction factor in the model for dynamic capillary pressure that is a function of initial water content.

Optimal design of porous structures for the fastest liquid absorption.

PubMed

Shou, Dahua; Ye, Lin; Fan, Jintu; Fu, Kunkun

2014-01-14

Porous materials engineered for rapid liquid absorption are useful in many applications, including oil recovery, spacecraft life-support systems, moisture management fabrics, medical wound dressings, and microfluidic devices. Dynamic absorption in capillary tubes and porous media is driven by the capillary pressure, which is inversely proportional to the pore size. On the other hand, the permeability of porous materials scales with the square of the pore size. The dynamic competition between these two superimposed mechanisms for liquid absorption through a heterogeneous porous structure may lead to an overall minimum absorption time. In this work, we explore liquid absorption in two different heterogeneous porous structures [three-dimensional (3D) circular tubes and porous layers], which are composed of two sections with variations in radius/porosity and height. The absorption time to fill the voids of porous constructs is expressed as a function of radius/porosity and height of local sections, and the absorption process does not follow the classic Washburn's law. Under given height and void volume, these two-section structures with a negative gradient of radius/porosity against the absorption direction are shown to have faster absorption rates than control samples with uniform radius/porosity. In particular, optimal structural parameters, including radius/porosity and height, are found that account for the minimum absorption time. The liquid absorption in the optimized porous structure is up to 38% faster than in a control sample. The results obtained can be used a priori for the design of porous structures with excellent liquid management property in various fields.

Fully packed capillary electrochromatographic microchip with self-assembly colloidal silica beads.

PubMed

Park, Jongman; Lee, Dami; Kim, Won; Horiike, Shigeyoshi; Nishimoto, Takahiro; Lee, Se Hwan; Ahn, Chong H

2007-04-15

A fully packed capillary electrochromatographic (CEC) microchip showing improved solution and chip handling was developed. Microchannels for the CEC microchip were patterned on a cyclic olefin copolymer substrate by injection molding and packed fully with 0.8-microm monodisperse colloidal silica beads utilizing a self-assembly packing technique. The silica packed chip substrate was covered and thermally press-bonded. After fabrication, the chip was filled with buffer solution by self-priming capillary action. The self-assembly packing at each channel served as a built-in nanofilter allowing quick loading of samples and running buffer solution without filtration. Because of a large surface area-to-volume ratio of the silica packing, reproducible control of electroosmotic flow was possible without leveling of the solutions in the reservoirs resulting 1.3% rsd in migration rate. The capillary electrophoretic separation characteristics of the chip were studied using fluorescein isothiocyanate (FITC)-derivatized amino acids as probe molecules. A mixture of FITC and four FITC-derivatized amino acids was successfully separated with 2-mm separation channel length.

Condensation in Nanoporous Packed Beds.

PubMed

Ally, Javed; Molla, Shahnawaz; Mostowfi, Farshid

2016-05-10

In materials with tiny, nanometer-scale pores, liquid condensation is shifted from the bulk saturation pressure observed at larger scales. This effect is called capillary condensation and can block pores, which has major consequences in hydrocarbon production, as well as in fuel cells, catalysis, and powder adhesion. In this study, high pressure nanofluidic condensation studies are performed using propane and carbon dioxide in a colloidal crystal packed bed. Direct visualization allows the extent of condensation to be observed, as well as inference of the pore geometry from Bragg diffraction. We show experimentally that capillary condensation depends on pore geometry and wettability because these factors determine the shape of the menisci that coalesce when pore filling occurs, contrary to the typical assumption that all pore structures can be modeled as cylindrical and perfectly wetting. We also observe capillary condensation at higher pressures than has been done previously, which is important because many applications involving this phenomenon occur well above atmospheric pressure, and there is little, if any, experimental validation of capillary condensation at such pressures, particularly with direct visualization.

Quantification in an Introductory Diffusion Experiment.

ERIC Educational Resources Information Center

Snow, George E.

1991-01-01

Describes a take-home experiment in which students measure the diffusion of acid through acid-filled capillary tubes immersed into base solutions and vice versa. Students represent and analyze the effects of ambient temperature, molecular weight, and concentrations of the solutions on that movement. (MDH)

A two-angle model of dynamic wetting in microscale capillaries under low capillary numbers with experiments.

PubMed

Lei, Da; Lin, Mian; Li, Yun; Jiang, Wenbin

2018-06-15

An accurate model of the dynamic contact angle θ d is critical for the calculation of capillary force in applications like enhanced oil recovery, where the capillary number Ca ranges from 10 -10 to 10 -5 and the Bond number Bo is less than 10 -4 . The rate-dependence of the dynamic contact angle under such conditions remains blurred, and is the main target of this study. Featuring with pressure control and interface tracking, the innovative experimental system presented in this work achieves the desired ranges of Ca and Bo, and enables the direct optical measurement of dynamic contact angles in capillaries as tiny as 40 × 20 (width × height) μm and 80 × 20 μm. The advancing and receding processes of wetting and nonwetting liquids were tested. The dynamic contact angle was confirmed velocity-independent with 10 -9  < Ca < 10 -5 (contact line velocity V = 0.135-490 μm/s) and it can be described by a two-angle model with desirable accuracy. A modified two-angle model was developed and an empirical form was obtained from experiments. For different liquids contacting the same surface, the advancing angle θ adv approximately equals the static contact angle θ o . The receding angle θ rec was found to be a linear function of θ adv , in good agreement with our and other experiments from the literature. Copyright © 2018 Elsevier Inc. All rights reserved.

Six orders of magnitude dynamic range in capillary electrophoresis with ultrasensitive laser-induced fluorescence detection

PubMed Central

Whitmore, Colin D.; Essaka, David; Dovichi, Norman J.

2009-01-01

An ultrasensitive laser-induced fluorescence detector was used with capillary electrophoresis for the study of 5-carboxy-tetramethylrhodamine. The raw signal from the detector provided roughly three orders of magnitude dynamic range. The signal saturated at high analyte concentrations due to the dead time associated with the single-photon counting avalanche photodiode employed in the detector. The signal can be corrected for the detector dead time, providing an additional order of magnitude dynamic range. To further increase dynamic range, two fiber-optic beam-splitters were cascaded to generate a primary signal and two attenuated signals, each monitored by a single-photon counting avalanche photodiode. The combined signals from the three photodiodes are reasonably linear from the concentration detection limit of 3 pM to 10 μM, the maximum concentration investigated, a range of 3,000,000. Mass detection limits were 150 yoctomoles injected onto the capillary. PMID:19836546

Modifying effect of dynamic space flight factors on radiation damage of air-dry seeds of Crepis capillaris (L) Wallr.

PubMed

Vaulina, E N; Kostina, L N

1975-01-01

The influence of dynamic factors (vibration and linear acceleration) on the rate of chromosome aberrations in Crepis capillaris was studied. The vibrational process simulated was similar in its characteristics to that occurring at the launch of spaceships. In combination with linear acceleration it caused a statistically significant increase in the rate of chromosome aberrations compared with the control (R=7.70). The dynamic factors modified the effect of radiation damage induced by acute gamma-irradiation (3 krad). Pre-radiation treatment with vibration and acceleration on the seeds caused a significant decrease (R=10.23) of the effect of radiation damage, from 15.57% to 9.74%. The post-radiation treatment of C. capillaris seeds with the dynamic factors did not change the rate of chromosome aberrations significantly (from 15.57% to 15.90%).

Numerical studies of the surface tension effect of cryogenic liquid helium

NASA Technical Reports Server (NTRS)

Hung, R. J.

1994-01-01

The generalized mathematical formulation of sloshing dynamics for partially filled liquid of cryogenic superfluid helium II in dewar containers driven by both the gravity gradient and jitter accelerations applicable to scientific spacecraft which is eligible to carry out spinning motion and/or slew motion for the purpose of performing scientific observation during the normal spacecraft operation is investigated. An example is given with Gravity Probe-B (GP-B) spacecraft which is responsible for the sloshing dynamics. The jitter accelerations include slew motion, spinning motion, atmospheric drag on the spacecraft, spacecraft attitude motions arising from machinery vibrations, thruster firing, pointing control of spacecraft, crew motion, etc. Explicit mathematical expressions to cover these forces acting on the spacecraft fluid systems are derived. The numerical computation of sloshing dynamics has been based on the non-inertia frame spacecraft bound coordinate, and solve time-dependent, three-dimensional formulations of partial differential equations subject to initial and boundary conditions. The explicit mathematical expressions of boundary conditions to cover capillary force effect on the liquid vapor interface in microgravity environments are also derived. The formulations of fluid moment and angular moment fluctuations in fluid profiles induced by the sloshing dynamics, together with fluid stress and moment fluctuations exerted on the spacecraft dewar containers, have been derived.

Imbibition of ``Open Capillary'': Fundamentals and Applications

NASA Astrophysics Data System (ADS)

Tani, Marie; Kawano, Ryuji; Kamiya, Koki; Okumura, Ko

2015-11-01

Control or transportation of small amount of liquid is one of the most important issues in various contexts including medical sciences or pharmaceutical industries to fuel delivery. We studied imbibition of ``open capillary'' both experimentally and theoretically, and found simple scaling laws for both statics and dynamics of the imbibition, similarly as that of imbibition of capillary tubes. Furthermore, we revealed the existence of ``precursor film,'' which developed ahead of the imbibing front, and the dynamics of it is described well by another scaling law for capillary rise in a corner. Then, to show capabilities of open capillaries, we demonstrated two experiments by fabricating micro mixing devices to achieve (1) simultaneous multi-color change of the Bromothymol blue (BTB) solution and (2) expression of the green florescent protein (GFP). This research was partly supported by ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan). M. T. is supported by the Japan Society for the Promotion of Science Research Fellowships for Young Scientists.

Surface-dependent chemical equilibrium constants and capacitances for bare and 3-cyanopropyldimethylchlorosilane coated silica nanochannels.

PubMed

Andersen, Mathias Bækbo; Frey, Jared; Pennathur, Sumita; Bruus, Henrik

2011-01-01

We present a combined theoretical and experimental analysis of the solid-liquid interface of fused-silica nanofabricated channels with and without a hydrophilic 3-cyanopropyldimethylchlorosilane (cyanosilane) coating. We develop a model that relaxes the assumption that the surface parameters C(1), C(2), and pK(+) are constant and independent of surface composition. Our theoretical model consists of three parts: (i) a chemical equilibrium model of the bare or coated wall, (ii) a chemical equilibrium model of the buffered bulk electrolyte, and (iii) a self-consistent Gouy-Chapman-Stern triple-layer model of the electrochemical double layer coupling these two equilibrium models. To validate our model, we used both pH-sensitive dye-based capillary filling experiments as well as electro-osmotic current-monitoring measurements. Using our model we predict the dependence of ζ potential, surface charge density, and capillary filling length ratio on ionic strength for different surface compositions, which can be difficult to achieve otherwise. Copyright © 2010 Elsevier Inc. All rights reserved.

Capillary Flow in Containers of Polygonal Section: Theory and Experiment

NASA Technical Reports Server (NTRS)

Weislogel, Mark M.; Rame, Enrique (Technical Monitor)

2001-01-01

An improved understanding of the large-length-scale capillary flows arising in a low-gravity environment is critical to that engineering community concerned with the design and analysis of spacecraft fluids management systems. Because a significant portion of liquid behavior in spacecraft is capillary dominated it is natural to consider designs that best exploit the spontaneous character of such flows. In the present work, a recently verified asymptotic analysis is extended to approximate spontaneous capillary flows in a large class of cylindrical containers of irregular polygonal section experiencing a step reduction in gravitational acceleration. Drop tower tests are conducted using partially-filled irregular triangular containers for comparison with the theoretical predictions. The degree to which the experimental data agree with the theory is a testament to the robustness of the basic analytical assumption of predominantly parallel flow. As a result, the closed form analytical expressions presented serve as simple, accurate tools for predicting bulk flow characteristics essential to practical low-g system design and analysis. Equations for predicting corner wetting rates, total container flow rates, and transient surfaces shapes are provided that are relevant also to terrestrial applications such as capillary flow in porous media.

Dynamical clustering of red blood cells in capillary vessels.

PubMed

Boryczko, Krzysztof; Dzwinel, Witold; Yuen, David A

2003-02-01

We have modeled the dynamics of a 3-D system consisting of red blood cells (RBCs), plasma and capillary walls using a discrete-particle approach. The blood cells and capillary walls are composed of a mesh of particles interacting with harmonic forces between nearest neighbors. We employ classical mechanics to mimic the elastic properties of RBCs with a biconcave disk composed of a mesh of spring-like particles. The fluid particle method allows for modeling the plasma as a particle ensemble, where each particle represents a collective unit of fluid, which is defined by its mass, moment of inertia, translational and angular momenta. Realistic behavior of blood cells is modeled by considering RBCs and plasma flowing through capillaries of various shapes. Three types of vessels are employed: a pipe with a choking point, a curved vessel and bifurcating capillaries. There is a strong tendency to produce RBC clusters in capillaries. The choking points and other irregularities in geometry influence both the flow and RBC shapes, considerably increasing the clotting effect. We also discuss other clotting factors coming from the physical properties of blood, such as the viscosity of the plasma and the elasticity of the RBCs. Modeling has been carried out with adequate resolution by using 1 to 10 million particles. Discrete particle simulations open a new pathway for modeling the dynamics of complex, viscoelastic fluids at the microscale, where both liquid and solid phases are treated with discrete particles. Figure A snapshot from fluid particle simulation of RBCs flowing along a curved capillary. The red color corresponds to the highest velocity. We can observe aggregation of RBCs at places with the most stagnant plasma flow.

Nano-Wilhelmy investigation of dynamic wetting properties of AFM tips through tip-nanobubble interaction

PubMed Central

Wang, Yuliang; Wang, Huimin; Bi, Shusheng; Guo, Bin

2016-01-01

The dynamic wetting properties of atomic force microscopy (AFM) tips are of much concern in many AFM-related measurement, fabrication, and manipulation applications. In this study, the wetting properties of silicon and silicon nitride AFM tips are investigated through dynamic contact angle measurement using a nano-Wilhelmy balance based method. This is done by capillary force measurement during extension and retraction motion of AFM tips relative to interfacial nanobubbles. The working principle of the proposed method and mathematic models for dynamic contact angle measurement are presented. Geometric models of AFM tips were constructed using scanning electronic microscopy (SEM) images taken from different view directions. The detailed process of tip-nanobubble interaction was investigated using force-distance curves of AFM on nanobubbles. Several parameters including nanobubble height, adhesion and capillary force between tip and nanobubbles are extracted. The variation of these parameters was studied over nanobubble surfaces. The dynamic contact angles of the AFM tips were calculated from the capillary force measurements. The proposed method provides direct measurement of dynamic contact angles for AFM tips and can also be taken as a general approach for nanoscale dynamic wetting property investigation. PMID:27452115

Nano-Wilhelmy investigation of dynamic wetting properties of AFM tips through tip-nanobubble interaction

NASA Astrophysics Data System (ADS)

Wang, Yuliang; Wang, Huimin; Bi, Shusheng; Guo, Bin

2016-07-01

The dynamic wetting properties of atomic force microscopy (AFM) tips are of much concern in many AFM-related measurement, fabrication, and manipulation applications. In this study, the wetting properties of silicon and silicon nitride AFM tips are investigated through dynamic contact angle measurement using a nano-Wilhelmy balance based method. This is done by capillary force measurement during extension and retraction motion of AFM tips relative to interfacial nanobubbles. The working principle of the proposed method and mathematic models for dynamic contact angle measurement are presented. Geometric models of AFM tips were constructed using scanning electronic microscopy (SEM) images taken from different view directions. The detailed process of tip-nanobubble interaction was investigated using force-distance curves of AFM on nanobubbles. Several parameters including nanobubble height, adhesion and capillary force between tip and nanobubbles are extracted. The variation of these parameters was studied over nanobubble surfaces. The dynamic contact angles of the AFM tips were calculated from the capillary force measurements. The proposed method provides direct measurement of dynamic contact angles for AFM tips and can also be taken as a general approach for nanoscale dynamic wetting property investigation.

LASER PLASMA AND LASER APPLICATIONS: Plasma transparency in laser absorption waves in metal capillaries

NASA Astrophysics Data System (ADS)

Anisimov, V. N.; Kozolupenko, A. P.; Sebrant, A. Yu

1988-12-01

An experimental investigation was made of the plasma transparency to heating radiation in capillaries when absorption waves propagated in these capillaries as a result of interaction with a CO2 laser pulse of 5-μs duration. When the length of the capillary was in excess of 20 mm, total absorption of the radiation by the plasma was observed at air pressures of 1-100 kPa. When the capillary length was 12 mm, a partial recovery of the transparency took place. A comparison was made with the dynamics and recovery of the plasma transparency when breakdown of air took place near the free surface.

Capillary flow weld-bonding

NASA Technical Reports Server (NTRS)

Vaughan, R. W.; Jones, R. J. (Inventor)

1976-01-01

The invention of a weld-bonding technique for titanium plates was described. This involves fastening at least two plates of titanium together using spot-welding and applying a bead of adhesive along the edge of the resistance spot-welded joint which upon heating, flows and fills the separation between the joint components.

A novel method for calculating the dynamic capillary force and correcting the pressure error in micro-tube experiment.

PubMed

Wang, Shuoliang; Liu, Pengcheng; Zhao, Hui; Zhang, Yuan

2017-11-29

Micro-tube experiment has been implemented to understand the mechanisms of governing microcosmic fluid percolation and is extensively used in both fields of micro electromechanical engineering and petroleum engineering. The measured pressure difference across the microtube is not equal to the actual pressure difference across the microtube. Taking into account the additional pressure losses between the outlet of the micro tube and the outlet of the entire setup, we propose a new method for predicting the dynamic capillary pressure using the Level-set method. We first demonstrate it is a reliable method for describing microscopic flow by comparing the micro-model flow-test results against the predicted results using the Level-set method. In the proposed approach, Level-set method is applied to predict the pressure distribution along the microtube when the fluids flow along the microtube at a given flow rate; the microtube used in the calculation has the same size as the one used in the experiment. From the simulation results, the pressure difference across a curved interface (i.e., dynamic capillary pressure) can be directly obtained. We also show that dynamic capillary force should be properly evaluated in the micro-tube experiment in order to obtain the actual pressure difference across the microtube.

Tuning and Freezing Disorder in Photonic Crystals using Percolation Lithography.

PubMed

Burgess, Ian B; Abedzadeh, Navid; Kay, Theresa M; Shneidman, Anna V; Cranshaw, Derek J; Lončar, Marko; Aizenberg, Joanna

2016-01-21

Although common in biological systems, synthetic self-assembly routes to complex 3D photonic structures with tailored degrees of disorder remain elusive. Here we show how liquids can be used to finely control disorder in porous 3D photonic crystals, leading to complex and hierarchical geometries. In these optofluidic crystals, dynamically tunable disorder is superimposed onto the periodic optical structure through partial wetting or evaporation. In both cases, macroscopic symmetry breaking is driven by subtle sub-wavelength variations in the pore geometry. These variations direct site-selective infiltration of liquids through capillary interactions. Incorporating cross-linkable resins into our liquids, we developed methods to freeze in place the filling patterns at arbitrary degrees of partial wetting and intermediate stages of drying. These percolation lithography techniques produced permanent photonic structures with adjustable disorder. By coupling strong changes in optical properties to subtle differences in fluid behavior, optofluidic crystals may also prove useful in rapid analysis of liquids.

A Microsystem Based on Porous Silicon-Glass Anodic Bonding for Gas and Liquid Optical Sensing

PubMed Central

De Stefano, Luca; Malecki, Krzysztof; Della Corte, Francesco G.; Moretti, Luigi; Rea, Ilaria; Rotiroti, Lucia; Rendina, Ivo

2006-01-01

We have recently presented an integrated silicon-glass opto-chemical sensor for lab-on-chip applications, based on porous silicon and anodic bonding technologies. In this work, we have optically characterized the sensor response on exposure to vapors of several organic compounds by means of reflectivity measurements. The interaction between the porous silicon, which acts as transducer layer, and the organic vapors fluxed into the glass sealed microchamber, is preserved by the fabrication process, resulting in optical path increase, due to the capillary condensation of the vapors into the pores. Using the Bruggemann theory, we have calculated the filled pores volume for each substance. The sensor dynamic has been described by time-resolved measurements: due to the analysis chamber miniaturization, the response time is only of 2 s. All these results have been compared with data acquired on the same PSi structure before the anodic bonding process.

Competition between anisotropic viscous fingers

NASA Astrophysics Data System (ADS)

Pecelerowicz, M.; Budek, A.; Szymczak, P.

2014-09-01

We consider viscous fingers created by injection of low viscosity fluid into the network of capillaries initially filled with a more viscous fluid (motor oil). Due to the anisotropy of the system and its geometry, such a setup promotes the formation of long-and-thin fingers which then grow and compete for the available flow, interacting through the pressure field. The interaction between the fingers is analyzed using the branched growth formalism of Halsey and Leibig (Phys. Rev. A 46, 7723, 1992) using a number of simple, analytically tractable models. It is shown that as soon as the fingers are allowed to capture the flow from one another, the fixed point appears in the phase space, corresponding to the asymptotic state in which the growth of one of the fingers in hindered by the other. The properties of phase space flows in such systems are shown to be remarkably insensitive to the details of the dynamics.

Transport through a network of capillaries from ultrametric diffusion equation with quadratic nonlinearity

NASA Astrophysics Data System (ADS)

Oleschko, K.; Khrennikov, A.

2017-10-01

This paper is about a novel mathematical framework to model transport (of, e.g., fluid or gas) through networks of capillaries. This framework takes into account the tree structure of the networks of capillaries. (Roughly speaking, we use the tree-like system of coordinates.) As is well known, tree-geometry can be topologically described as the geometry of an ultrametric space, i.e., a metric space in which the metric satisfies the strong triangle inequality: in each triangle, the third side is less than or equal to the maximum of two other sides. Thus transport (e.g., of oil or emulsion of oil and water in porous media, or blood and air in biological organisms) through networks of capillaries can be mathematically modelled as ultrametric diffusion. Such modelling was performed in a series of recently published papers of the authors. However, the process of transport through capillaries can be only approximately described by the linear diffusion, because the concentration of, e.g., oil droplets, in a capillary can essentially modify the dynamics. Therefore nonlinear dynamical equations provide a more adequate model of transport in a network of capillaries. We consider a nonlinear ultrametric diffusion equation with quadratic nonlinearity - to model transport in such a network. Here, as in the linear case, we apply the theory of ultrametric wavelets. The paper also contains a simple introduction to theory of ultrametric spaces and analysis on them.

Nonequilibrium capillarity effects in multiphase flow through small volume fractured porous media

NASA Astrophysics Data System (ADS)

Tang, M.; Zhan, H.; Lu, S.

2017-12-01

Analyzing and understanding the capillary pressure curves in fractured porous media is a crucial subject in a number of industrial applications, such as crude oil recovery in the fractured reservoir, CO2 sequestration in fractured brine aquifers and shale gas development. Many studies have observed the significant nonequilibrium capillarity effects in multiphase flow through porous media and proposed that conventional equilibrium capillary pressure may not accurately describe transient two-phase flow behavior under dynamical conditions. To date, only several laboratory experiments and numerical models have been conducted into investigating the characteristic of nonequilibrium capillary pressure in unfractured porous media, a clear picture of the effects of fractures on the dynamic capillary pressure in fractured porous media remains elusive. In this study, four digital porous models were built based on CT image data from ZEISS Xradia 520 Versa CT scanning, a series of direct simulations of multiphase flow in fractured porous media were carried out based on lattice Boltzmann method and three-dimensional porous models. The results show that both the aperture and orientation of the fractures have significant effects on the nonequilibrium capillary pressure coefficients and multiphase flow behaviors. The nonequilibrium capillary pressure coefficients in fractured porous media are one to two orders of magnitude lower than unfractured porous media. This study presents a new direct simulation based methodology for the detailed analysis of nonequilibrium capillary pressure in fractured porous media.

Automated one-step DNA sequencing based on nanoliter reaction volumes and capillary electrophoresis.

PubMed

Pang, H M; Yeung, E S

2000-08-01

An integrated system with a nano-reactor for cycle-sequencing reaction coupled to on-line purification and capillary gel electrophoresis has been demonstrated. Fifty nanoliters of reagent solution, which includes dye-labeled terminators, polymerase, BSA and template, was aspirated and mixed with the template inside the nano-reactor followed by cycle-sequencing reaction. The reaction products were then purified by a size-exclusion chromatographic column operated at 50 degrees C followed by room temperature on-line injection of the DNA fragments into a capillary for gel electrophoresis. Over 450 bases of DNA can be separated and identified. As little as 25 nl reagent solution can be used for the cycle-sequencing reaction with a slightly shorter read length. Significant savings on reagent cost is achieved because the remaining stock solution can be reused without contamination. The steps of cycle sequencing, on-line purification, injection, DNA separation, capillary regeneration, gel-filling and fluidic manipulation were performed with complete automation. This system can be readily multiplexed for high-throughput DNA sequencing or PCR analysis directly from templates or even biological materials.

Time resolved EUV spectra from Zpinching capillary discharge plasma

NASA Astrophysics Data System (ADS)

Jancarek, Alexandr; Nevrkla, Michal; Nawaz, Fahad

2015-09-01

We developed symmetrically charged driver to obtain high voltage, high current Z-pinching capillary discharge. Plasma is created by up to 70 kA, 29 ns risetime current pulse passing through a 5 mm inner diameter, 224 mm long capillary filled with gas to initial pressure in the range of 1 kPa. Due to the low inductance design of the driver, the pinch is observable directly from the measured current curve. Time-integrated and time-resolved spectra of discharge plasma radiation are recorded together with the capillary current and analyzed. The most encouraging spectra were captured in the wavelength range 8.3 ÷ 14 nm. This spectral region contains nitrogen Balmer series lines including potentially lasing NVII 2 - 3 transition. Spectral lines are identified in the NIST database using the FLY kinetic code. The line of 13.38 nm wavelength, transition NVII 2 - 3, was observed in gated, and also in time-integrated spectra for currents >60 kA. This work has been supported by the Ministry of Education, Youth and Sports of the Czech Republic grants LG13029.

Regioisomeric and enantiomeric analyses of 24 designer cathinones and phenethylamines using ultra high performance liquid chromatography and capillary electrophoresis with added cyclodextrins.

PubMed

Li, Li; Lurie, Ira S

2015-09-01

DESIGNER: phenethylamines (PEAs) and cathinones have been encountered worldwide. Complete characterization of these substances can be challenging due to their chirality and variably substituted phenyl rings. In this study, 24 PEAs and cathinones were analyzed by ultra high performance liquid chromatography with photo diode array detection (UHPLC-PDA) on a variety of stationary phases, and by capillary electrophoresis on a dynamically coated capillary with PDA detection (CE-PDA). In the UHPLC-PDA study, a BEH Phenyl column resolved 18 of the 24 regioisomers in 8min, with good discrimination of the PEAs. In contrast, capillary zone electrophoresis (CZE) on a dynamically coated capillary partially or baseline resolved only 10 of the 24 regioisomers, but with improved discrimination of mono-substituted cathinones. A second series of CE-PDA experiments using 80mM (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) in the run buffer resolved all 24 regioisomers and all but two sets of enantiomers within 18min. Five illicit samples were successfully analyzed using the described methods. Published by Elsevier Ireland Ltd.

Superfluid helium sloshing dynamics induced oscillations and fluctuations of angular momentum, force and moment actuated on spacecraft driven by gravity gradient or jitter acceleration associated with slew motion

NASA Technical Reports Server (NTRS)

Hung, R. J.

1994-01-01

The generalized mathematical formulation of sloshing dynamics for partially filled liquid of cryogenic superfluid helium II in dewar containers driven by the gravity gradient and jitter accelerations associated with slew motion for the purpose to perform scientific observation during the normal spacecraft operation are investigated. An example is given with the Advanced X-Ray Astrophysics Facility-Spectroscopy (AXAF-S) for slew motion which is responsible for the sloshing dynamics. The jitter accelerations include slew motion, spinning motion, atmospheric drag on the spacecraft, spacecraft attitude motions arising from machinery vibrations, thruster firing, pointing control of spacecraft, crew motion, etc. Explicit mathematical expressions to cover these forces acting on the spacecraft fluid systems are derived. The numerical computation of sloshing dynamics is based on the non-inertia frame spacecraft bound coordinate, and solve time-dependent, three-dimensional formulations of partial differential equations subject to initial and boundary conditions. The explicit mathematical expressions of boundary conditions to cover capillary force effect on the liquid-vapor interface in microgravity environments are also derived. The formulations of fluid moment and angular moment fluctuations in fluid profiles induced by the sloshing dynamics, together with fluid stress and moment fluctuations exerted on the spacecraft dewar containers have also been derived. Examples are also given for cases applicable to the AXAF-S spacecraft sloshing dynamics associated with slew motion.

Liquid Microjunction Surface Sampling Probe Fluid Dynamics: Computational and Experimental Analysis of Coaxial Intercapillary Positioning Effects on Sample Manipulation

DOE Office of Scientific and Technical Information (OSTI.GOV)

ElNaggar, Mariam S; Barbier, Charlotte N; Van Berkel, Gary J

A coaxial geometry liquid microjunction surface sampling probe (LMJ-SSP) enables direct extraction of analytes from surfaces for subsequent analysis by techniques like mass spectrometry. Solution dynamics at the probe-to-sample surface interface in the LMJ-SSP has been suspected to influence sampling efficiency and dispersion but has not been rigorously investigated. The effect on flow dynamics and analyte transport to the mass spectrometer caused by coaxial retraction of the inner and outer capillaries from each other and the surface during sampling with a LMJ-SSP was investigated using computational fluid dynamics and experimentation. A transparent LMJ-SSP was constructed to provide the means formore » visual observation of the dynamics of the surface sampling process. Visual observation, computational fluid dynamics (CFD) analysis, and experimental results revealed that inner capillary axial retraction from the flush position relative to the outer capillary transitioned the probe from a continuous sampling and injection mode through an intermediate regime to sample plug formationmode caused by eddy currents at the sampling end of the probe. The potential for analytical implementation of these newly discovered probe operational modes is discussed.« less

Determining lead, cadmium and mercury in cosmetics using sweeping via dynamic chelation by capillary electrophoresis.

PubMed

Chen, Kuan-Ling; Jiang, Shiuh-Jen; Chen, Yen-Ling

2017-03-01

International limits have been established for metal impurities in cosmetics to prevent overexposure to heavy metal ions. Sweeping via dynamic chelation was developed using capillary electrophoresis to analyze lead (Pb), cadmium (Cd) and mercury (Hg) impurities in cosmetics. The sweeping via dynamic chelation mechanism involves a large volume of metal ions being swept by a small quantity of chelating agents that were electrokinetically injected into the capillary to chelate metal ions and increase the detection sensitivity. The optimized conditions were as follows: Firstly, the capillary was rinsed by a 0.6 mM TTAB solution to reverse the EOF. The sample solution, which was diluted using 25 mM ammonium acetate (pH 6.0), was injected into the capillary using a pressure of 3.5 psi for 99.9 s. Then, EDTA was injected at -25 kV for 1 min from the EDTA buffer (25 mM ammonium acetate containing 0.6 mM TTAB and 5 mM EDTA), and the metal ions were swept and stacked simultaneously. Finally, the separation was performed at -20 kV using a separation buffer (100 mM ammonium acetate (pH 6.0)). A small quantity of chelating agents introduced into the capillary could yield 33-, 50- and 100-fold detection improvements for Pb, Cd and Hg, respectively, more sensitive than conventional capillary zone electrophoresis. Correlation coefficients greater than 0.998 indicated that this method exhibited good linearity. The relative standard deviation and relative error were less than 8.7%, indicating high precision and accuracy. The recovery value of the homemade lotion, which was employed to simulate the real sample matrix, was 93-104%, which indicated that the sample matrix does not affect the quantitative results. Finally, commercial cosmetics were employed to demonstrate the feasibility of the method to determine Pb, Cd and Hg without complicated sample pretreatment. Graphical Abstract The procedure of analyzing metal ions in cosmetics by sweeping via dynamic chelation.

Comparison of influence of incorporated 3d-, 4d- and 4f-metal chlorides on electronic properties of single-walled carbon nanotubes

NASA Astrophysics Data System (ADS)

Kharlamova, M. V.

2013-06-01

In the present work, the channels of single-walled carbon nanotubes were filled with melts of ZnCl2, CdCl2, and TbCl3 by a capillary method with subsequent slow cooling. The detailed study of electronic structure of filled nanotubes was performed using Raman, optical absorption, and X-ray photoelectron spectroscopy. The obtained data are in mutual agreement and it proves that the filling of carbon nanotube channels with all these salts leads to the charge transfer from nanotube walls to the incorporated compounds, thus acceptor doping of nanotubes takes place. It was found out that encapsulated terbium chloride has the largest influence on the electronic properties of carbon nanotubes.

Automatic cryogenic liquid level controller is safe for use near combustible substances

NASA Technical Reports Server (NTRS)

Krejsa, M.

1966-01-01

Automatic mechanical liquid level controller that is independent of any external power sources is used with safety in the presence of combustibles. A gas filled capillary tube which leads from a pressurized chamber, is inserted into the cryogenic liquid reservoir and becomes a liquid level sensing element or probe.

Confinement-Driven Phase Separation of Quantum Liquid Mixtures

NASA Astrophysics Data System (ADS)

Prisk, T. R.; Pantalei, C.; Kaiser, H.; Sokol, P. E.

2012-08-01

We report small-angle neutron scattering studies of liquid helium mixtures confined in Mobil Crystalline Material-41 (MCM-41), a porous silica glass with narrow cylindrical nanopores (d=3.4nm). MCM-41 is an ideal model adsorbent for fundamental studies of gas sorption in porous media because its monodisperse pores are arranged in a 2D triangular lattice. The small-angle scattering consists of a series of diffraction peaks whose intensities are determined by how the imbibed liquid fills the pores. Pure He4 adsorbed in the pores show classic, layer-by-layer film growth as a function of pore filling, leaving the long range symmetry of the system intact. In contrast, the adsorption of He3-He4 mixtures produces a structure incommensurate with the pore lattice. Neither capillary condensation nor preferential adsorption of one helium isotope to the pore walls can provide the symmetry-breaking mechanism. The scattering is consistent with the formation of randomly distributed liquid-liquid microdomains ˜2.3nm in size, providing evidence that confinement in a nanometer scale capillary can drive local phase separation in quantum liquid mixtures.

Liquid redistribution behind a drainage front in porous media imaged by neutron radiography

NASA Astrophysics Data System (ADS)

Hoogland, Frouke; Lehmann, Peter; Moebius, Franziska; Vontobel, Peter; Or, Dani

2013-04-01

Drainage from porous media is a highly dynamic process involving the motion of a displacement front with rapid pore scale interfacial jumps and phase entrapment, but also a more gradual host of liquid redistribution processes in the unsaturated region behind the front. Depending on the velocity of the drainage process, liquid properties and the permeability of the porous medium, redistribution lingers long after the main drainage process is stopped, until gravity and capillary forces regain equilibrium. The rapid and often highly inertial Haines jumps at the drainage front challenge the validity of Buckingham-Darcy law and thus representation of the process based on the foundation of Richards equation. To quantify front displacement and liquid reconfiguration and to test validity of Richards equation with respect to fast drainage dynamics, we carried out drainage experiments by withdrawing water from the bottom of initially saturated sand-filled Hele-Shaw cells at constant water flux (2.6 or 13.1 mm/minute). Water content distribution and evolution of drainage front were measured with neutron radiography at spatial and temporal resolutions of 0.1 mm and 3 seconds, respectively. Water pressure was measured above and below the front using pressure transducers and a tensiometer. After the pump was stopped (at a front depth around 100 mm), capillary pressure values in the unsaturated region (above the front) gradually converged to a new equilibrium. The pressure signal in the saturated region below the front reflected viscous losses during flow that were relaxed when the pump stopped. During pressure relaxation water was redistributed primarily downward in the unsaturated region. Pressure signals and dynamics of water content profiles for fast process (13.6 mm/minute) could not be reproduced with Richards equation based on hydraulic functions determined in preceding laboratory experiments. To explore if the deviations stem from inappropriate hydraulic functions we redefined them based on fitting the slow experiment (2.6 mm/min) and apply the optimized functions for the fast experiment. Finally we will discuss application of alternative formulation based on foam drainage equation to represent liquid redistribution dynamics behind the front.

On Thermocapillary Mechanism of Spatial Separation of Metal Melts

NASA Astrophysics Data System (ADS)

Demin, V. A.; Mizev, A. I.; Petukhov, M. I.

2018-02-01

Theoretical research has been devoted to the study of binary metal melts behavior in a thin capillary. Earlier it has been found experimentally that unusually significant and quick redistribution of melts components takes place along capillary after the cooling. Numerical simulation of concentration-induced convection has been carried out to explain these experimental data. Two-component melt of both liquid metals filling vertical thin capillary with non-uniform temperature distribution on the boundaries is considered. It is assumed that the condition of absolute non-wetting is valid on the sidewalls. Because of this effect there is a free surface on vertical boundaries, where thermocapillary force is appeared due to the external longitudinal temperature gradient. It makes to move liquid elements at a big distance, compared with axial size of capillary. Effects of adsorption-desorption on the surface, thermal and concentration-capillary forces, convective motion in a volume and diffusion generate the large-scale circulation. This process includes the admixture carrying-out on the surface in the more hot higher part of the channel, its following transfer down along the boundary due to the thermocapillary force and its return in the volume over the desorption in the lower part of capillary. Intensity of motion and processes of adsorption-desorption on the free boundary have the decisive influence upon the formation of concentration fields and speed of components redistribution. Thus, one of the possible mechanisms of longitudinal division on components of liquid binary mixtures in thin channels has been demonstrated.

A catalytic chiral gel microfluidic reactor assembled via dynamic covalent chemistry† †Electronic supplementary information (ESI) available: Experimental details, additional characterization and catalytic data. See DOI: 10.1039/c5sc00314h Click here for additional data file.

PubMed Central

Liu, Haoliang; Feng, Juan; Chen, Liuping

2015-01-01

A novel dynamic covalent gel strategy is reported to immobilize an asymmetric catalyst within the channels of a microfluidic flow reactor. A layer of a catalytically active Mn–salen dynamic covalent imine gel matrix was coated onto a functionalized capillary. Mn–salen active moiety was incorporated into dynamic covalent imine gel matrix via the reaction of a chiral Mn–salen dialdehyde unit with a tetraamine linker. The catalytic activity of the capillary reactor has been demonstrated in enantioselective kinetic resolution of secondary alcohols. PMID:28706652

Rare-earth metal halogenide encapsulation-induced modifications in Raman spectra of single-walled carbon nanotubes

NASA Astrophysics Data System (ADS)

Kharlamova, M. V.

2015-01-01

In the present work, a detailed Raman spectroscopy investigation on the single-walled carbon nanotubes (SWCNTs) filled with praseodymium chloride, terbium chloride and thulium chloride was performed. The salts were incorporated inside the SWCNTs by a capillary filling method using melts, and the high-resolution transmission electron microscopy data proved the high filling degree of the nanotube channels. A thorough analysis of the radial breathing mode and G-band of the Raman spectra of the pristine and filled SWCNTs showed that the encapsulated salts cause acceptor doping of the host nanotubes, and the doping efficiency depends on the compound. The incorporated thulium chloride has the strongest doping effect on the SWCNTs, whereas praseodymium chloride has the weakest effect. It was found that the encapsulated salts modify more significantly the electronic structure of metallic nanotubes than semiconducting SWCNTs.

Filling carbon nanotubes with particles.

PubMed

Kim, Byong M; Qian, Shizhi; Bau, Haim H

2005-05-01

The filling of carbon nanotubes (CNTs) with fluorescent particles was studied experimentally and theoretically. The fluorescent signals emitted by the particles were visible through the walls of the nanotubes, and the particles inside the tubes were observable with an electron microscope. Taking advantage of the template-grown carbon nanotubes' transparency to fluorescent light, we measured the filling rate of the tubes with particles at room conditions. Liquids such as ethylene glycol, water, and ethylene glycol/water mixtures, laden with 50 nm diameter fluorescent particles, were brought into contact with 500 nm diameter CNTs. The liquid and the particles' transport were observed, respectively, with optical and fluorescence microscopy. The CNTs were filled controllably with particles by the complementary action of capillary forces and the evaporation of the liquid. The experimental results were compared and favorably agreed with theoretical predictions. This is the first report on fluorescence studies of particle transport in carbon nanotubes.

Acquisition system environmental effects study. [for capillary-screen propellant retention devices

NASA Technical Reports Server (NTRS)

1975-01-01

The effects of vibration, warm gas exposure, and feed system startup/shutdown fluid dynamics on capillary-screen propellant retention capabilities are quantified. The existing technology is extended to the point where quantitative conlusions in terms of design criteria may be drawn.

Treelike networks accelerating capillary flow.

PubMed

Shou, Dahua; Ye, Lin; Fan, Jintu

2014-05-01

Transport in treelike networks has received wide attention in natural systems, oil recovery, microelectronic cooling systems, and textiles. Existing studies are focused on transport behaviors under a constant potential difference (including pressure, temperature, and voltage) in a steady state [B. Yu and B. Li, Phys. Rev. E 73, 066302 (2006); J. Chen, B. Yu, P. Xu, and Y. Li, Phys. Rev. E 75, 056301 (2007)]. However, dynamic (time-dependent) transport in such systems has rarely been concerned. In this work, we theoretically investigate the dynamics of capillary flow in treelike networks and design the distribution of radius and length of local branches for the fastest capillary flow. It is demonstrated that capillary flow in the optimized tree networks is faster than in traditional parallel tube nets under fixed constraints. As well, the flow time of the liquid is found to increase approximately linearly with penetration distance, which differs from Washburn's classic description that flow time increases as the square of penetration distance in a uniform tube.

Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.

PubMed

Horka, Marie; Ruzicka, Filip; Horký, Jaroslav; Holá, Veronika; Slais, Karel

2006-12-15

The nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. This dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. The values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pI markers and were found comparable with pI of the native compounds. The mixed cultures of proteins and microorganisms, Escherichia coli CCM 3954, Staphylococcus epidermidis CCM 4418, Proteus vulgaris, Enterococcus faecalis CCM 4224, and Stenotrophomonas maltophilia, the strains of the yeast cells, Candida albicans CCM 8180, Candida krusei, Candida parapsilosis, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae were reproducibly focused and separated by the suggested technique. Using UV excitation for the on-column fluorometric detection, the minimum detectable amount was down to 10 cells injected on the separation capillary.

The effect of concentration in the patterning of silica particles by the soft lithographic technique

NASA Astrophysics Data System (ADS)

Singh, Akanksha; Malek, Chantal Khan; Kulkarni, Sulabha K.

2008-12-01

Soft lithography provides remarkable surface patterning techniques to organize colloidal particles for a wide variety of applications. In particular, micromolding in capillaries (MIMIC) has emerged as a patterning method in the nanometer to micrometer scale in a single step by using templating and directing nanoparticles via capillary forces in the channel. The present work reports the results of the micropatterning of monodispersed silica particles of ~338 ± 2 nm size in ethanol medium, using MIMIC on silicon substrates. The effect of the concentration of silica particles on the patterning has been investigated. The patterns are well aligned and completely filled at 2 wt% concentration of silica particles.

Liquid film drag out in the presence of molecular forces

NASA Astrophysics Data System (ADS)

Schmidhalter, I.; Cerro, R. L.; Giavedoni, M. D.; Saita, F. A.

2013-03-01

From a practical as well as a conceptual point of view, one of the most interesting problems of physicochemical hydrodynamics is the drag out of a liquid film by a moving solid out of a pool of liquid. The basic problem, sometimes denoted the Landau-Levich problem [L. Landau and B. Levich, "Dragging of a liquid by a moving plate," Acta Physicochim. USSR 17, 42-54 (1942)], involves an interesting blend of capillary and viscous forces plus a matching of the static solution for capillary rise with a numerical solution of the film evolution equation, neglecting gravity, on the downstream region of the flow field. The original solution describes experimental data for a wide range of Capillary numbers but fails to match results for large and very small Capillary numbers. Molecular level forces are introduced to create an augmented version of the film evolution equation to show the effect of van der Waals forces at the lower range of Capillary numbers. A closed form solution for static capillary rise, including molecular forces, was matched with a numerical solution of the augmented film evolution equation in the dynamic meniscus region. Molecular forces do not sensibly modify the static capillary rise region, since film thicknesses are larger than the range of influence of van der Waals forces, but are determinant in shaping the downstream dynamic meniscus of the very thin liquid films. As expected, a quantitatively different level of disjoining pressure for different values of molecular constants remains in the very thin liquid film far downstream. Computational results for a wide range of Capillary numbers and Hamaker constants show a clear transition towards a region where the film thickness becomes independent of the coating speed.

Progression of Diabetic Capillary Occlusion: A Model

PubMed Central

Gens, John Scott; Glazier, James A.; Burns, Stephen A.; Gast, Thomas J.

2016-01-01

An explanatory computational model is developed of the contiguous areas of retinal capillary loss which play a large role in diabetic maculapathy and diabetic retinal neovascularization. Strictly random leukocyte mediated capillary occlusion cannot explain the occurrence of large contiguous areas of retinal ischemia. Therefore occlusion of an individual capillary must increase the probability of occlusion of surrounding capillaries. A retinal perifoveal vascular sector as well as a peripheral retinal capillary network and a deleted hexagonal capillary network are modelled using Compucell3D. The perifoveal modelling produces a pattern of spreading capillary loss with associated macular edema. In the peripheral network, spreading ischemia results from the progressive loss of the ladder capillaries which connect peripheral arterioles and venules. System blood flow was elevated in the macular model before a later reduction in flow in cases with progression of capillary occlusions. Simulations differing only in initial vascular network structures but with identical dynamics for oxygen, growth factors and vascular occlusions, replicate key clinical observations of ischemia and macular edema in the posterior pole and ischemia in the retinal periphery. The simulation results also seem consistent with quantitative data on macular blood flow and qualitative data on venous oxygenation. One computational model applied to distinct capillary networks in different retinal regions yielded results comparable to clinical observations in those regions. PMID:27300722

Capillary Movement in Substrates in Microgravity

NASA Technical Reports Server (NTRS)

Bula, R. J.; Duffie, N. A.

1996-01-01

A more complete understanding of the dynamics of capillary flow through an unsaturated porous medium would be useful for a number of space and terrestrial applications. Knowledge of capillary migration of liquids in granular beds in microgravity would significantly enhance the development and understanding of how a matrix based nutrient delivery system for the growth of plants would function in a microgravity environment. Thus, such information is of interest from the theoretical as well as practical point of view.

Integrated microfluidic chip for rapid DNA digestion and time-resolved capillary electrophoresis analysis

PubMed Central

Lin, Che-Hsin; Wang, Yao-Nan; Fu, Lung-Ming

2012-01-01

An integrated microfluidic chip is proposed for rapid DNA digestion and time-resolved capillary electrophoresis (CE) analysis. The chip comprises two gel-filled chambers for DNA enrichment and purification, respectively, a T-form micromixer for DNA/restriction enzyme mixing, a serpentine channel for DNA digestion reaction, and a CE channel for on-line capillary electrophoresis analysis. The DNA and restriction enzyme are mixed electroomostically using a pinched-switching DC field. The experimental and numerical results show that a mixing performance of 97% is achieved within a distance of 1 mm from the T-junction when a driving voltage of 90 V/cm and a switching frequency of 4 Hz are applied. Successive mixing digestion and capillary electrophoresis operation clearly present the changes on digesting φx-174 DNA in different CE runs. The time-resolved electropherograms show that the proposed device enables a φx-174 DNA sample comprising 11 fragments to be concentrated and analyzed within 24 min. Overall, the results presented in this study show that the proposed microfluidic chip provides a rapid and effective tool for DNA digestion and CE analysis applications. PMID:22662085

Influence of steric hindrance on enantioseparation of Dns-amino acids and pesticides on terguride based chiral selectors in capillary electrophoresis.

PubMed

Honzátko, Ales; Cvak, Jan; Vaingátová, Silvie; Flieger, Miroslav

2005-05-01

Three urea derivatives of ergoline-based chiral selectors (CSs), differing in the size of the urea side chain, i.e. dimethyl- (CSI), diethyl- (CSII), and diisopropylurea (CSIII), were used to study the effect of steric hindrance on the enantioseparation of dansyl amino acids (Dns-AAs), pesticides, and mandelic acid under condition of capillary electrophoresis (CE) in linear polyacrylamide coated capillaries. A mixture of organic modifiers (MeOH/THF, 4:1 v/v) in a BGE consisting of 100 mM beta-alanine-acetate was used to increase the solubility of CSs up to 25 mM. The capillary was filled with CS (high UV absorption), and the inlet and outlet vials contained buffer solutions only. The best enantioseparation of Dns-AAs was achieved on CSI. Increased steric hindrance of the chiral binding site led to reduction of both enantioselectivity and resolution. The opposite pattern was observed for the separation of mandelic acid enantiomers, where the best enantioseparation and resolution was obtained with CSIII. Most of the pesticides studied reached maximum selectivity on the diethylurea ergoline derivative (CSII). Enantioseparation of fenoxaprop was found to be independent of steric hindrance.

Analysis of Cement-Based Pastes Mixed with Waste Tire Rubber

NASA Astrophysics Data System (ADS)

Sola, O. C.; Ozyazgan, C.; Sayin, B.

2017-03-01

Using the methods of thermal gravimetry, differential thermal analysis, Furier transform infrared analysis, and capillary absorption, the properties of a cement composite produced by introducing waste tyre rubber into a cement mixture were investigated. It was found that the composite filled with the rubber had a much lower water absorption ability than the unfilled one.

Liquid-Vapor Coexistence at a Mesoporous Substrate

NASA Astrophysics Data System (ADS)

Kityk, A. V.; Hofmann, T.; Knorr, K.

2008-01-01

The condensation of hexane vapor onto a mesoporous Si substrate with a pore radius of 3.5 nm has been studied by means of volumetry and ellipsometry. The filling fraction of the pores and the coverage of the substrate have been determined. The coverage of the regime after the completion of capillary condensation has been compared to recent theoretical work.

Improvement of chromatographic performances of in-situ synthesized hybrid C8 silica monoliths by reduction of structural radial heterogeneities.

PubMed

Roux, R; Abi Jaoudé, M; Demesmay, C

2009-05-01

Several modifications of a previously described protocol are proposed to improve the performances of in-situ synthesized C(8) hybrid silica monoliths. Our attention was focused on reducing the sources of radial heterogeneity that may be responsible for the poor efficiencies observed in the hydrodynamic elution mode. It was demonstrated that a decrease in the temperature of the capillary during the filling step equally to that of the polymerization mixture (0 degrees C), associated with a decrease of the gelation temperature to 20 degrees C along with a new pre-treatment of the capillary's internal walls [with a mixture of tetraethoxysilane (TEOS)/EtOH (1/3, v/v)] allows (i) increasing the radial homogeneity of the monolith, thus further enhancing the performances in the nano-liquid chromatography (nano-LC) mode, (ii) improving the capillary to capillary reproducibility in terms of permeability and efficiencies. In fact, the average minimum plate height H(min) was lowered from 24 to 14 microm and the capillary-to-capillary reproducibility of the synthesis was widely improved by factors two and three of reduction on the calculated standard deviation, respectively for both the efficiency in the nano-LC mode and the permeability. At last, the improved radial homogeneity and anchoring of the synthesized monoliths allowed increasing the inner diameter of the capillary (up to 150 microm) without any significant loss in efficiency. Finally, long term stability of the as-obtained monolithic stationary phases in terms of retention and efficiency was studied. In addition, the evaluation of their chromatographic behaviour was also achieved with the Tanaka test and the results were compared to those already published for commercial monoliths (Chromolith) as well as for particulate stationary phases.

Collective motion of macroscopic spheres floating on capillary ripples: Dynamic heterogeneity and dynamic criticality

NASA Astrophysics Data System (ADS)

Sanlı, Ceyda; Saitoh, Kuniyasu; Luding, Stefan; van der Meer, Devaraj

2014-09-01

When a densely packed monolayer of macroscopic spheres floats on chaotic capillary Faraday waves, a coexistence of large scale convective motion and caging dynamics typical for glassy systems is observed. We subtract the convective mean flow using a coarse graining (homogenization) method and reveal subdiffusion for the caging time scales followed by a diffusive regime at later times. We apply the methods developed to study dynamic heterogeneity and show that the typical time and length scales of the fluctuations due to rearrangements of observed particle groups significantly increase when the system approaches its largest experimentally accessible packing concentration. To connect the system to the dynamic criticality literature, we fit power laws to our results. The resultant critical exponents are consistent with those found in densely packed suspensions of colloids.

Collective motion of macroscopic spheres floating on capillary ripples: dynamic heterogeneity and dynamic criticality.

PubMed

Sanlı, Ceyda; Saitoh, Kuniyasu; Luding, Stefan; van der Meer, Devaraj

2014-09-01

When a densely packed monolayer of macroscopic spheres floats on chaotic capillary Faraday waves, a coexistence of large scale convective motion and caging dynamics typical for glassy systems is observed. We subtract the convective mean flow using a coarse graining (homogenization) method and reveal subdiffusion for the caging time scales followed by a diffusive regime at later times. We apply the methods developed to study dynamic heterogeneity and show that the typical time and length scales of the fluctuations due to rearrangements of observed particle groups significantly increase when the system approaches its largest experimentally accessible packing concentration. To connect the system to the dynamic criticality literature, we fit power laws to our results. The resultant critical exponents are consistent with those found in densely packed suspensions of colloids.

The unsaturated flow in porous media with dynamic capillary pressure

NASA Astrophysics Data System (ADS)

Milišić, Josipa-Pina

2018-05-01

In this paper we consider a degenerate pseudoparabolic equation for the wetting saturation of an unsaturated two-phase flow in porous media with dynamic capillary pressure-saturation relationship where the relaxation parameter depends on the saturation. Following the approach given in [13] the existence of a weak solution is proved using Galerkin approximation and regularization techniques. A priori estimates needed for passing to the limit when the regularization parameter goes to zero are obtained by using appropriate test-functions, motivated by the fact that considered PDE allows a natural generalization of the classical Kullback entropy. Finally, a special care was given in obtaining an estimate of the mixed-derivative term by combining the information from the capillary pressure with the obtained a priori estimates on the saturation.

[Advances of the coatings used in columns for capillary electrophoresis and in nanochannels of chips].

PubMed

Liu, Chunye; Chen, Jierong

2005-01-01

An overview is provided on the advancement and development of coating preparation methodology and materials used in capillaries and channels in microfluidic chip. Discussion is also given on the effects of coatings in the resolutions of separation and the reproducibility of separations. Dynamic coatings and linked coatings, classified as homo-polymers, copolymers and heterocyclic compounds, are further discussed, and so are the methods for the preparation of the coatings by cross-linked reaction, sol-gel process, photomodification and chemical deposition, etc. The discussion will be useful for the optimization of capillary columns that are used in capillary electrophoresis and nanochannels of chip.

Lennard-Jones fluids in two-dimensional nano-pores. Multi-phase coexistence and fluid structure

NASA Astrophysics Data System (ADS)

Yatsyshin, Petr; Savva, Nikos; Kalliadasis, Serafim

2014-03-01

We present a number of fundamental findings on the wetting behaviour of nano-pores. A popular model for fluid confinement is a one-dimensional (1D) slit pore formed by two parallel planar walls and it exhibits capillary condensation (CC): a first-order phase transition from vapour to capillary-liquid (Kelvin shift). Capping such a pore at one end by a third orthogonal wall forms a prototypical two-dimensional (2D) pore. We show that 2D pores possess a wetting temperature such that below this temperature CC remains of first order, above it becomes a continuous phase transition manifested by a slab of capillary-liquid filling the pore from the capping wall. Continuous CC exhibits hysteresis and can be preceded by a first-order capillary prewetting transition. Additionally, liquid drops can form in the corners of the 2D pore (remnant of 2D wedge prewetting). The three fluid phases, vapour, capillary-liquid slab and corner drops, can coexist at the pore triple point. Our model is based on the statistical mechanics of fluids in the density functional formulation. The fluid-fluid and fluid-substrate interactions are dispersive. We analyze in detail the microscopic fluid structure, isotherms and full phase diagrams. Our findings also suggest novel ways to control wetting of nano-pores. We are grateful to the European Research Council via Advanced Grant No. 247031 for support.

Preparation and evaluation of molecularly imprinted polymers based on 9-ethyladenine for the recognition of nucleotide bases in capillary electrochromatography.

PubMed

Huang, Yi-Chen; Lin, Chun-Chi; Liu, Chuen-Ying

2004-02-01

A molecularly imprinted polymer (MIP) comprising 9-ethyladenine was polymerized in situ inside the capillary for the electrochromatographic separation of nucleotide bases. The capillary wall was first functionalized with 3-trimethoxysilylpropyl methacrylate (10% v/v) and 1,1-diphenyl-2-picrylhydrazyl (0.01% w/v) in toluene. Following this treatment, the capillary was filled with acetonitrile containing 9-ethyladenine, methacrylic acid, ethylene glycol dimethacrylate, and initiator. After polymerization, the MIP was shrunk into a film against the inner wall of the capillary with the syringe pump. The template was then removed with methanol under nitrogen flow. For evaluation the feasibility of the MIP column for the separation of nucleotide bases, some parameters including the pH, concentration of the background electrolyte, the applied voltage as well as the effect of organic modifier were studied. The migration behavior of nucleotide bases on the MIP column was also compared with that on the bare fused-silica column. The results indicated that the MIP columns demonstrated better recognition properties at a pH range of 6-8. The efficiency (plates/m) at pH 8 for the nonimprinted analyte was 75,300 for cytosine, 50,200 for thymine, and 14,800 for guanine. However, the efficiency for the imprinted analyte, adenine, was quite low. This was evidenced by the broad peak, yielding only 2600 plates/m.

Free Surface Flows and Extensional Rheology of Polymer Solutions

NASA Astrophysics Data System (ADS)

Dinic, Jelena; Jimenez, Leidy Nallely; Biagioli, Madeleine; Estrada, Alexandro; Sharma, Vivek

Free-surface flows - jetting, spraying, atomization during fuel injection, roller-coating, gravure printing, several microfluidic drop/particle formation techniques, and screen-printing - all involve the formation of axisymmetric fluid elements that spontaneously break into droplets by a surface-tension-driven instability. The growth of the capillary-driven instability and pinch-off dynamics are dictated by a complex interplay of inertial, viscous and capillary stresses for simple fluids. Additional contributions by elasticity, extensibility and extensional viscosity play a role for complex fluids. We show that visualization and analysis of capillary-driven thinning and pinch-off dynamics of the columnar neck in an asymmetric liquid bridge created by dripping-onto-substrate (DoS) can be used for characterizing the extensional rheology of complex fluids. Using a wide variety of complex fluids, we show the measurement of the extensional relaxation time, extensional viscosity, power-law index and shear viscosity. Lastly, we elucidate how polymer composition, flexibility, and molecular weight determine the thinning and pinch-off dynamics of polymeric complex fluids.

Dynamics and Stability of Capillary Surfaces: Liquid Switches at Small Scales

NASA Technical Reports Server (NTRS)

Steen, Paul H.; Bhandar, Anand; Vogel, Michael J.; Hirsa, Amir H.

2004-01-01

The dynamics and stability of systems of interfaces is central to a range of technologies related to the Human Exploration and Development of Space (HEDS). Our premise is that dramatic shape changes can be manipulated to advantage with minimal input, if the system is near instability. The primary objective is to develop the science base to allow novel approaches to liquid management in low-gravity based on this premise. HEDS requires efficient, reliable and lightweight technologies. Our poster will highlight our progress toward this goal using the capillary switch as an example. A capillary surface is a liquid/liquid or liquid/gas interface whose shape is determined by surface tension. For typical liquids (e.g., water) against gas on earth, capillary surfaces occur on the millimeterscale and smaller where shape deformation due to gravity is unimportant. In low gravity, they can occur on the centimeter scale. Capillary surfaces can be combined to make a switch a system with multiple stable states. A capillary switch can generate motion or effect force. To be practical, the energy barriers of such a switch must be tunable, its switching time (kinetics) short and its triggering mechanism reliable. We illustrate these features with a capillary switch that consists of two droplets, coupled by common pressure. As long as contact lines remained pinned, motions are inviscid, even at sub-millimeter scales, with consequent promise of low-power consumption at the device level. Predictions of theory are compared to experiment on i) a soap-film prototype at centimeter scale and ii) a liquid droplet switch at millimeter-scale.

Uptake and withdrawal of droplets from carbon nanotubes.

PubMed

Schebarchov, D; Hendy, S C

2011-01-01

We give an account of recent studies of droplet uptake and withdrawal from carbon nanotubes using simple theoretical arguments and molecular dynamics simulations. Firstly, the thermodynamics of droplet uptake and release is considered and tested via simulation. We show that the Laplace pressure acting on a droplet assists capillary uptake, allowing sufficiently small non-wetting droplets to be absorbed. We then demonstrate how the uptake and release of droplets of non-wetting fluids can be exploited for the use of carbon nanotubes as nanopipettes. Finally, we extend the Lucas-Washburn model to deal with the dynamics of droplet capillary uptake, and again test this by comparison with molecular dynamics simulations.

Uptake and withdrawal of droplets from carbon nanotubes

NASA Astrophysics Data System (ADS)

Schebarchov, D.; Hendy, S. C.

2011-01-01

We give an account of recent studies of droplet uptake and withdrawal from carbon nanotubes using simple theoretical arguments and molecular dynamics simulations. Firstly, the thermodynamics of droplet uptake and release is considered and tested via simulation. We show that the Laplace pressure acting on a droplet assists capillary uptake, allowing sufficiently small non-wetting droplets to be absorbed. We then demonstrate how the uptake and release of droplets of non-wetting fluids can be exploited for the use of carbon nanotubes as nanopipettes. Finally, we extend the Lucas-Washburn model to deal with the dynamics of droplet capillary uptake, and again test this by comparison with molecular dynamics simulations.

Effect of wave action on near-well zone cleaning

NASA Astrophysics Data System (ADS)

Pen'kovskii, V. I.; Korsakova, N. K.

2017-10-01

Drilling filtrate invasion into the producing formation and native water accumulating of the near-well zone in well operation reduce the well productivity. As a result of that, depending on characteristic capillary pressure scale and differential pressure drawdown, oil production rate may become lower than expected one. In this paper, it is considered the hysteresis effects of capillary pressure after reversion of displacement. As applied to laboratory experiment conditions, the solution of problem of oil flow in formation model with a pressure drop on the model sides harmonically varied with time is presented. It was estimated a range of fluid vibration effective action on the near-well zone cleaning from capillary locking water. The plant simulating extraction of oil from formation using widely practised sucker-rod pump has been created. Formation model is presented as a slot filled with broken glass between two plates. In the process, natural oil and sodium chloride solution were used as working fluids. The experiments qualitatively confirm a positive effect of jack pumps on the near-well zone cleaning.

Fabrication of self-enclosed nanochannels based on capillary-pressure balance mechanism

NASA Astrophysics Data System (ADS)

Kou, Yu; Sang, Aixia; Li, Xin; Wang, Xudi

2017-10-01

Polymer-based micro/nano fluidic devices are becoming increasingly important to biological applications and fluidic control. In this paper, we propose a self-enclosure method for the fabrication of large-area nanochannels without external force by using a capillary-pressure balance mechanism. The melt polymer coated on the nanogrooves fills into the trenches inevitably and the air in the trenches is not excluded but compressed, which leads to an equilibrium state between pressure of the trapped air and capillary force of melt polymer eventually, resulting in the channels’ formation. A pressure balance model was proposed to elucidate the unique self-sealing phenomenon and the criteria for the design and construction of sealed channels was discussed. According to the bonding mechanism investigated using the volume of fluid (VOF) simulation and experiments, we can control the dimension of sealed channels by varying the baking condition. This fabrication technique has great potential for low-cost and mass production of polymeric-based micro/nano fluidic devices.

Wettability and Flow Rate Impacts on Immiscible Displacement: A Theoretical Model

NASA Astrophysics Data System (ADS)

Hu, Ran; Wan, Jiamin; Yang, Zhibing; Chen, Yi-Feng; Tokunaga, Tetsu

2018-04-01

When a more viscous fluid displaces a less viscous one in porous media, viscous pressure drop stabilizes the displacement front against capillary pressure fluctuation. For this favorable viscous ratio conditions, previous studies focused on the front instability under slow flow conditions but did not address competing effects of wettability and flow rate. Here we study how this competition controls displacement patterns. We propose a theoretical model that describes the crossover from fingering to stable flow as a function of invading fluid contact angle θ and capillary number Ca. The phase diagram predicted by the model shows that decreasing θ stabilizes the displacement for θ≥45° and the critical contact angle θc increases with Ca. The boundary between corner flow and cooperative filling for θ < 45° is also described. This work extends the classic phase diagram and has potential applications in predicting CO2 capillary trapping and manipulating wettability to enhance gas/oil displacement efficiency.

Field-Scale Modeling of Local Capillary Trapping During CO2 Injection into a Saline Aquifer

NASA Astrophysics Data System (ADS)

Ren, B.; Lake, L. W.; Bryant, S. L.

2015-12-01

Local capillary trapping is the small-scale (10-2 to 10+1 m) CO2 trapping that is caused by the capillary pressure heterogeneity. The benefit of LCT, applied specially to CO2 sequestration, is that saturation of stored CO2 is larger than the residual gas, yet these CO2 are not susceptible to leakage through failed seals. Thus quantifying the extent of local capillary trapping is valuable in design and risk assessment of geologic storage projects. Modeling local capillary trapping is computationally expensive and may even be intractable using a conventional reservoir simulator. In this paper, we propose a novel method to model local capillary trapping by combining geologic criteria and connectivity analysis. The connectivity analysis originally developed for characterizing well-to-reservoir connectivity is adapted to this problem by means of a newly defined edge weight property between neighboring grid blocks, which accounts for the multiphase flow properties, injection rate, and gravity effect. Then the connectivity is estimated from shortest path algorithm to predict the CO2 migration behavior and plume shape during injection. A geologic criteria algorithm is developed to estimate the potential local capillary traps based only on the entry capillary pressure field. The latter is correlated to a geostatistical realization of permeability field. The extended connectivity analysis shows a good match of CO2 plume computed by the full-physics simulation. We then incorporate it into the geologic algorithm to quantify the amount of LCT structures identified within the entry capillary pressure field that can be filled during CO2 injection. Several simulations are conducted in the reservoirs with different level of heterogeneity (measured by the Dykstra-Parsons coefficient) under various injection scenarios. We find that there exists a threshold Dykstra-Parsons coefficient, below which low injection rate gives rise to more LCT; whereas higher injection rate increases LCT in heterogeneous reservoirs. Both the geologic algorithm and connectivity analysis are very fast; therefore, the integrated methodology can be used as a quick tool to estimate local capillary trapping. It can also be used as a potential complement to the full-physics simulation to evaluate safe storage capacity.

State diagram for adhesion dynamics of deformable capsules under shear flow.

PubMed

Luo, Zheng Yuan; Bai, Bo Feng

2016-08-17

Due to the significance of understanding the underlying mechanisms of cell adhesion in biological processes and cell capture in biomedical applications, we numerically investigate the adhesion dynamics of deformable capsules under shear flow by using a three-dimensional computational fluid dynamic model. This model is based on the coupling of the front tracking-finite element method for elastic mechanics of the capsule membrane and the adhesion kinetics simulation for adhesive interactions between capsules and functionalized surfaces. Using this model, three distinct adhesion dynamic states are predicted, such as detachment, rolling and firm-adhesion. Specifically, the effects of capsule deformability quantified by the capillary number on the transitions of these three dynamic states are investigated by developing an adhesion dynamic state diagram for the first time. At low capillary numbers (e.g. Ca < 0.0075), whole-capsule deformation confers the capsule a flattened bottom in contact with the functionalized surface, which hence promotes the rolling-to-firm-adhesion transition. It is consistent with the observations from previous studies that cell deformation promotes the adhesion of cells lying in the rolling regime. However, it is surprising to find that, at relatively high capillary numbers (e.g. 0.0075 < Ca < 0.0175), the effect of capsule deformability on its adhesion dynamics is far more complex than just promoting adhesion. High deformability of capsules makes their bottom take a concave shape with no adhesion bond formation in the middle. The appearance of this specific capsule shape inhibits the transitions of both rolling-to-firm-adhesion and detachment-to-rolling, and it means that capsule deformation no longer promotes the capsule adhesion. Besides, it is interesting to note that, when the capillary number exceeds a critical value (e.g. Ca = 0.0175), the rolling state no longer appears, since capsules exhibit large deviation from the spherical shape.

Sol-gel approach to in situ creation of high pH-resistant surface-bonded organic-inorganic hybrid zirconia coating for capillary microextraction (in-tube SPME).

PubMed

Alhooshani, Khalid; Kim, Tae-Young; Kabir, Abuzar; Malik, Abdul

2005-01-07

A novel zirconia-based hybrid organic-inorganic sol-gel coating was developed for capillary microextraction (CME) (in-tube SPME). High degree of chemical inertness inherent in zirconia makes it very difficult to covalently bind a suitable organic ligand to its surface. In the present work, this problem was addressed from a sol-gel chemistry point of view. Principles of sol-gel chemistry were employed to chemically bind a hydroxy-terminated silicone polymer (polydimethyldiphenylsiloxane, PDMDPS) to a sol-gel zirconia network in the course of its evolution from a highly reactive alkoxide precursor undergoing controlled hydrolytic polycondensation reactions. A fused silica capillary was filled with a properly designed sol solution to allow for the sol-gel reactions to take place within the capillary for a predetermined period of time (typically 15-30 min). In the course of this process, a layer of the evolving hybrid organic-inorganic sol-gel polymer got chemically anchored to the silanol groups on the capillary inner walls via condensation reaction. At the end of this in-capillary residence time, the unbonded part of the sol solution was expelled from the capillary under helium pressure, leaving behind a chemically bonded sol-gel zirconia-PDMDPS coating on the inner walls. Polycyclic aromatic hydrocarbons, ketones, and aldehydes were efficiently extracted and preconcentrated from dilute aqueous samples using sol-gel zirconia-PDMDPS coated capillaries followed by thermal desorption and GC analysis of the extracted solutes. The newly developed sol-gel hybrid zirconia coatings demonstrated excellent pH stability, and retained the extraction characteristics intact even after continuous rinsing with a 0.1 M NaOH solution for 24 h. To our knowledge, this is the first report on the use of a sol-gel zirconia-based hybrid organic-inorganic coating as an extraction medium in solid phase microextraction (SPME).

Applications of liquid-based separation in conjunction with mass spectrometry to the analysis of forensic evidence.

PubMed

Moini, Mehdi

2018-05-01

In the past few years, there has been a significant effort by the forensic science community to develop new scientific techniques for the analysis of forensic evidence. Forensic chemists have been spearheaded to develop information-rich confirmatory technologies and techniques and apply them to a broad array of forensic challenges. The purpose of these confirmatory techniques is to provide alternatives to presumptive techniques that rely on data such as color changes, pattern matching, or retention time alone, which are prone to more false positives. To this end, the application of separation techniques in conjunction with mass spectrometry has played an important role in the analysis of forensic evidence. Moreover, in the past few years the role of liquid separation techniques, such as liquid chromatography and capillary electrophoresis in conjunction with mass spectrometry, has gained significant tractions and have been applied to a wide range of chemicals, from small molecules such as drugs and explosives, to large molecules such as proteins. For example, proteomics and peptidomics have been used for identification of humans, organs, and bodily fluids. A wide range of HPLC techniques including reversed phase, hydrophilic interaction, mixed-mode, supercritical fluid, multidimensional chromatography, and nanoLC, as well as several modes of capillary electrophoresis mass spectrometry, including capillary zone electrophoresis, partial filling, full filling, and micellar electrokenetic chromatography have been applied to the analysis drugs, explosives, and questioned documents. In this article, we review recent (2015-2017) applications of liquid separation in conjunction with mass spectrometry to the analysis of forensic evidence. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The direct determination of dose-to-water using a water calorimeter.

PubMed

Schulz, R J; Wuu, C S; Weinhous, M S

1987-01-01

A flexible, temperature-regulated, water calorimeter has been constructed which consists of three nested cylinders. The innermost "core" is a 10 X 10 cm right cylinder made of glass, the contents of which are isolated from the environment. It has two Teflon-washered glass valves for filling, and two thermistors are supported at the center by glass capillary tubes. Surrounding the core is a "jacket" that provides approximately 2 cm of air insulation between the core and the "shield." The shield surrounds the jacket with a 2.5-cm layer of temperature-regulated water flowing at 51/min. The core is filled with highly purified water the gas content of which is established prior to filling. Convection currents, which may be induced by dose gradients or thermistor power dissipation, are eliminated by operating the calorimeter at 4 degrees C. Depending upon the power level of the thermistors, 15-200 microW, and the insulation provided by the glass capillary tubing, the temperature of the thermistors is higher than that of the surrounding water. To minimize potential errors caused by differences between calibration curves obtained at finite power levels, the zero-power-level calibration curve obtained by extrapolation is employed. Also the calorimeter response is corrected for the change in power level, and therefore thermistor temperature, that follows the resistance change caused by irradiation. The response of the calorimeter to 4-MV x rays has been compared to that of an ionization chamber irradiated in an identical geometry.(ABSTRACT TRUNCATED AT 250 WORDS)

Liquid crystal-based biosensor with backscattering interferometry: A quantitative approach.

PubMed

Khan, Mashooq; Park, Soo-Young

2017-01-15

We developed a new technology that uses backscattering interferometry (BSI) to quantitatively measure nematic liquid crystal (NLC)-based biosensors, those usually relied on texture reading for on/off signals. The LC-based BSI comprised an octadecyltrichlorosilane (OTS)-coated square capillary filled with 4-cyano-4'-pentylbiphenyl (5CB, a nematic LC at room temperature). The LC/water interface in the capillary was functionalized by a coating of poly(acrylicacid-b-4-cyanobiphenyl-4'-oxyundecylacrylate) (PAA-b-LCP) and immobilized with the enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP) through covalent linkage to the PAA chains (5CB PAA-GOx:HRP ) for glucose detection. Laser irradiation of the LC near the LC/water interface resulted in backscattered fringes with high contrast. The change in the spatial position of the fringes (because of the change in the orientation of the LC caused by the GOx:HRP enzymatic reaction of glucose) altered the output voltage of the photodetector when its active area was aligned with the edge of one of the fringes. The change in the intensity at the photodetector allowed the detection limit of the instrument to be as low as 0.008mM with a linear range of 0.02-9mM in a short response time (~60s). This LC-based BSI technique allows for quantitative, sensitive, selective, reproducible, easily obtainable, and interference-free detection in a large linear dynamic range and for practical applications with human serum. Copyright © 2016 Elsevier B.V. All rights reserved.

Experimental investigations on sodium-filled heat pipes

NASA Technical Reports Server (NTRS)

Dorner, S.; Reiss, F.; Schretzmann, K.

1977-01-01

The possibilities of producing heat pipes and, especially, the necessary capillary structures are discussed. Several types of heat pipes were made from stainless steel and tested at temperatures between 400 and 1055 deg C. The thermal power was determined by a calorimeter. Results indicate: bubble-free evaporation of sodium from rectangular open chennels is possible with a heat flux of more than 1,940 W/sq cm at 1055 C. The temperature drop along the tube could be measured only at low temperatures. A subdivided heat pipe worked against the gravitational field. A heat pipe with a capillary structure made of a rolled screen was supported by rings and bars operated at 250 W/sq cm heat flux in the evaporating region.

Powerless tunable photonic crystal with bistable color and millisecond switching.

PubMed

Chan, Chia-Tsung; Yeh, J Andrew

2011-07-04

This study demonstrated a tunable photonic crystal (PhC) with 70 nm-wide spectral tuning (535 nm to 605 nm) and 3 ms of response time. The tunable PhC is based on reciprocal capillary action of liquid in the nanoscale PhC voids. By wetting the porous silicon PhC with ethanol and water, the PhC can be bistably switched respectively between liquid-filled state (orange color) and vapor-filled state (yellow color). Owing to the energy barrier between the two wetting states, the tunable PhC can remain at either of the two states with no external power consumption.

Jet pump assisted artery

NASA Technical Reports Server (NTRS)

1975-01-01

A procedure for priming an arterial heat pump is reported; the procedure also has a means for maintaining the pump in a primed state. This concept utilizes a capillary driven jet pump to create the necessary suction to fill the artery. Basically, the jet pump consists of a venturi or nozzle-diffuser type constriction in the vapor passage. The throat of this venturi is connected to the artery. Thus vapor, gas, liquid, or a combination of the above is pumped continuously out of the artery. As a result, the artery is always filled with liquid and an adequate supply of working fluid is provided to the evaporator of the heat pipe.

A computational DFT study of structural transitions in textured solid-fluid interfaces

NASA Astrophysics Data System (ADS)

Yatsyshin, Petr; Parry, Andrew O.; Kalliadasis, Serafim

2015-11-01

Fluids adsorbed at walls, in capillary pores and slits, and in more exotic, sculpted geometries such as grooves and wedges can exhibit many new phase transitions, including wetting, pre-wetting, capillary-condensation and filling, compared to their bulk counterparts. As well as being of fundamental interest to the modern statistical mechanical theory of inhomogeneous fluids, these are also relevant to nanofluidics, chemical- and bioengineering. In this talk we will show using a microscopic Density Functional Theory (DFT) for fluids how novel, continuous, interfacial transitions associated with the first-order prewetting line, can occur on steps, in grooves and in wedges, that are sensitive to both the range of the intermolecular forces and interfacial fluctuation effects. These transitions compete with wetting, filling and condensation producing very rich phase diagrams even for relatively simple geometries. We will also discuss practical aspects of DFT calculations, and demonstrate how this statistical-mechanical framework is capable of yielding complex fluid structure, interfacial tensions, and regions of thermodynamic stability of various fluid configurations. As a side note, this demonstrates that DFT is an excellent tool for the investigations of complex multiphase systems. We acknowledge financial support from the European Research Council via Advanced Grant No. 247031.

Dynamics of ultra-thin polystyrene with and without a (artificial) dead layer studied by resonance enhanced dynamic light scattering

NASA Astrophysics Data System (ADS)

Vianna, S. D. B.; Lin, F. Y.; Plum, M. A.; Duran, H.; Steffen, W.

2017-05-01

Using non-invasive, marker-free resonance enhanced dynamic light scattering, the dynamics of capillary waves on ultrathin polystyrene films' coupling to the viscoelastic and mechanical properties have been studied. The dynamics of ultrathin polymer films is still debated. In particular the question of what influence either the solid substrate and/or the fluid-gas interface has on the dynamics and the mechanical properties of films of glass forming liquids as polymers is in the focus of the present research. As a consequence, e.g., viscosity close to interfaces and thus the average viscosity of very thin films are prone to change. This study is focused on atactic, non-entangled polystyrene thin films on the gold surface. A slow dynamic mode was observed with Vogel-Fulcher-Tammann temperature dependence, slowing down with decreasing film thickness. We tentatively attribute this relaxation mode to overdamped capillary waves because of its temperature dependence and the dispersion with a wave vector which was found. No signs of a more mobile layer at the air/polymer interface or of a "dead layer" at the solid/polymer interface were found. Therefore we investigated the influence of an artificially created dead layer on the capillary wave dynamics by introducing covalently bound polystyrene polymer brushes as anchors. The dynamics was slowed down to a degree more than expected from theoretical work on the increase of density close to the solid liquid interface—instead of a "dead layer" of 2 nm, the interaction seems to extend more than 10 nm into the polymer.

Viscosity and Wetting Property of Water Confined in Extended Nanospace Simultaneously Measured from Highly-Pressurized Meniscus Motion.

PubMed

Li, Lixiao; Kazoe, Yutaka; Mawatari, Kazuma; Sugii, Yasuhiko; Kitamori, Takehiko

2012-09-06

Understanding fluid and interfacial properties in extended nanospace (10-1000 nm) is important for recent advances of nanofluidics. We studied properties of water confined in fused-silica nanochannels of 50-1500 nm sizes with two types of cross-section: (1) square channel of nanoscale width and depth, and (2) plate channel of microscale width and nanoscale depth. Viscosity and wetting property were simultaneously measured from capillary filling controlled by megapascal external pressure. The viscosity increased in extended nanospace, while the wetting property was almost constant. Especially, water in the square nanochannels had much higher viscosity than the plate channel, which can be explained considering loosely coupled water molecules by hydrogen bond on the surface within 24 nm. This study suggests specificity of fluids two-dimensionally confined in extended nanoscale, in which the liquid is highly viscous by the specific water phase, while the wetting dynamics is governed by the well-known adsorbed water layer of several-molecules thickness.

Tuning and Freezing Disorder in Photonic Crystals using Percolation Lithography

PubMed Central

Burgess, Ian B.; Abedzadeh, Navid; Kay, Theresa M.; Shneidman, Anna V.; Cranshaw, Derek J.; Lončar, Marko; Aizenberg, Joanna

2016-01-01

Although common in biological systems, synthetic self-assembly routes to complex 3D photonic structures with tailored degrees of disorder remain elusive. Here we show how liquids can be used to finely control disorder in porous 3D photonic crystals, leading to complex and hierarchical geometries. In these optofluidic crystals, dynamically tunable disorder is superimposed onto the periodic optical structure through partial wetting or evaporation. In both cases, macroscopic symmetry breaking is driven by subtle sub-wavelength variations in the pore geometry. These variations direct site-selective infiltration of liquids through capillary interactions. Incorporating cross-linkable resins into our liquids, we developed methods to freeze in place the filling patterns at arbitrary degrees of partial wetting and intermediate stages of drying. These percolation lithography techniques produced permanent photonic structures with adjustable disorder. By coupling strong changes in optical properties to subtle differences in fluid behavior, optofluidic crystals may also prove useful in rapid analysis of liquids. PMID:26790372

Nanoporous Anodic Alumina Surface Modification by Electrostatic, Covalent, and Immune Complexation Binding Investigated by Capillary Filling.

PubMed

Eckstein, Chris; Acosta, Laura K; Pol, Laura; Xifré-Pérez, Elisabet; Pallares, Josep; Ferré-Borrull, Josep; Marsal, Lluis F

2018-03-28

The fluid imbibition-coupled laser interferometry (FICLI) technique has been applied to detect and quantify surface changes and pore dimension variations in nanoporous anodic alumina (NAA) structures. FICLI is a noninvasive optical technique that permits the determination of the NAA average pore radius with high accuracy. In this work, the technique is applied after each step of different surface modification paths of the NAA pores: (i) electrostatic immobilization of bovine serum albumin (BSA), (ii) covalent attachment of streptavidin via (3-aminipropyl)-triethoxysilane and glutaraldehyde grafting, and (iii) immune complexation. Results show that BSA attachment can be detected as a reduction in estimated radius from FICLI with high accuracy and reproducibility. In the case of the covalent attachment of streptavidin, FICLI is able to recognize a multilayer formation of the silane and the protein. For immune complexation, the technique is able to detect different antibody-antigen bindings and distinguish different dynamics among different immune species.

Direct Numerical Simulation of Low Capillary Number Pore Scale Flows

NASA Astrophysics Data System (ADS)

Esmaeilzadeh, S.; Soulaine, C.; Tchelepi, H.

2017-12-01

The arrangement of void spaces and the granular structure of a porous medium determines multiple macroscopic properties of the rock such as porosity, capillary pressure, and relative permeability. Therefore, it is important to study the microscopic structure of the reservoir pores and understand the dynamics of fluid displacements through them. One approach for doing this, is direct numerical simulation of pore-scale flow that requires a robust numerical tool for prediction of fluid dynamics and a detailed understanding of the physical processes occurring at the pore-scale. In pore scale flows with a low capillary number, Eulerian multiphase methods are well-known to produce additional vorticity close to the interface. This is mainly due to discretization errors which lead to an imbalance of capillary pressure and surface tension forces that causes unphysical spurious currents. At the pore scale, these spurious currents can become significantly stronger than the average velocity in the phases, and lead to unphysical displacement of the interface. In this work, we first investigate the capability of the algebraic Volume of Fluid (VOF) method in OpenFOAM for low capillary number pore scale flow simulations. Afterward, we compare VOF results with a Coupled Level-Set Volume of Fluid (CLSVOF) method and Iso-Advector method. It has been shown that the former one reduces the VOF's unphysical spurious currents in some cases, and both are known to capture interfaces sharper than VOF. As the conclusion, we will investigate that whether the use of CLSVOF or Iso-Advector will lead to less spurious velocities and more accurate results for capillary driven pore-scale multiphase flows or not. Keywords: Pore-scale multiphase flow, Capillary driven flows, Spurious currents, OpenFOAM

Influence of the capillary on the ignition of the transient spark discharge

NASA Astrophysics Data System (ADS)

Gerling, T.; Hoder, T.; Brandenburg, R.; Bussiahn, R.; Weltmann, K.-D.

2013-04-01

A self-pulsing negative dc discharge in argon generated in a needle-to-plane geometry at open atmosphere is investigated. Additionally, the needle electrode can be surrounded by a quartz capillary. It is shown that the relative position of the capillary end to the needle tip strongly influences the discharge inception and its spatio-temporal dynamics. Without the capillary for the selected working parameters a streamer corona is ignited, but when the capillary surrounds the needle, the transient spark (TS) discharge is ignited after a pre-streamer (PS) occurs. The time between PS and TS discharge depends on the relative capillary end position. The existence of the PS is confirmed by electro-optical characterization. Furthermore, spectrally and spatio-temporally resolved cross-correlation spectroscopy is applied to show the most active region of pre-phase emission activity as indicators for high local electric field strength. The results indicate that with a capillary in place, the necessary energy input of the pre-phase into the system is mainly reduced by additional electrical fields at the capillary edge. Even such a small change as a shift of dielectric surface close to the plasma largely changes the energy balance in the system.

Effect of using acetone and distilled water on the performance of open loop pulsating heat pipe (OLPHP) with different filling ratios

NASA Astrophysics Data System (ADS)

Rahman, Md. Lutfor; Afrose, Tonima; Tahmina, Halima Khatun; Rinky, Rumana Parvin; Ali, Mohammad

2016-07-01

Pulsating heat pipe (PHP) is a new innovation in the modern era of miniaturizes thermal management system for its higher heating and cooling capacity. The objective of this experiment is to observe the performance of open loop pulsating heat pipe using two fluids at different filling ratios. This OLPHP is a copper capillary tube of 2.5mm outer diameter and 2mm inner diameter. It consists of 8 loops where the evaporative section is 50mm, adiabatic section is 120mm and condensation section is 80mm. The experiment is conducted with distilled water and acetone at 40%, 50%, 60%, and 70% filling ratios where 0° (vertical) is considered as definite angle of inclination. Distilled water and acetone are selected as working fluids considering their different latent heat of vaporization and surface tension. It is found that acetone shows lower thermal resistance than water at all heat inputs. Best performance of acetone is attained at 70% filling ratio. Water displays better heat transfer capability at 50% filling ratio.

Monogroove liquid heat exchanger

NASA Technical Reports Server (NTRS)

Brown, Richard F. (Inventor); Edelstein, Fred (Inventor)

1990-01-01

A liquid supply control is disclosed for a heat transfer system which transports heat by liquid-vapor phase change of a working fluid. An assembly (10) of monogroove heat pipe legs (15) can be operated automatically as either heat acquisition devices or heat discharge sources. The liquid channels (27) of the heat pipe legs (15) are connected to a reservoir (35) which is filled and drained by respective filling and draining valves (30, 32). Information from liquid level sensors (50, 51) on the reservoir (35) is combined (60) with temperature information (55) from the liquid heat exchanger (12) and temperature information (56) from the assembly vapor conduit (42) to regulate filling and draining of the reservoir (35), so that the reservoir (35) in turn serves the liquid supply/drain needs of the heat pipe legs (15), on demand, by passive capillary action (20, 28).

Quantitative analysis of nailfold capillary morphology in patients with fibromyalgia

PubMed Central

Choi, Dug-Hyun

2015-01-01

Background/Aims Nailfold capillaroscopy (NFC) has been used to examine morphological and functional microcirculation changes in connective tissue diseases. It has been demonstrated that NFC patterns reflect abnormal microvascular dynamics, which may play a role in fibromyalgia (FM) syndrome. The aim of this study was to determine NFC patterns in FM, and their association with clinical features of FM. Methods A total of 67 patients with FM, and 30 age- and sex-matched healthy controls, were included. Nailfold capillary patterns were quantitatively analyzed using computerized NFC. The parameters of interest were as follows: number of capillaries within the central 3 mm, deletion score, apical limb width, capillary width, and capillary dimension. Capillary dimension was determined by calculating the number of capillaries using the Adobe Photoshop version 7.0. Results FM patients had a lower number of capillaries and higher deletion scores on NFC compared to healthy controls (17.3 ± 1.7 vs. 21.8 ± 2.9, p < 0.05; 2.2 ± 0.9 vs. 0.7 ± 0.6, p < 0.05, respectively). Both apical limb width (µm) and capillary width (µm) were significantly decreased in FM patients (1.1 ± 0.2 vs. 3.7 ± 0.6; 5.4 ± 0.5 vs. 7.5 ± 1.4, respectively), indicating that FM patients have abnormally decreased digital capillary diameter and density. Interestingly, there was no difference in capillary dimension between the two groups, suggesting that the length or tortuosity of capillaries in FM patients is increased to compensate for diminished microcirculation. Conclusions FM patients had altered capillary density and diameter in the digits. Diminished microcirculation on NFC may alter capillary density and increase tortuosity. PMID:26161020

Impairment of Skin Capillary Recruitment Precedes Chronic Complications in Patients with Type 1 Diabetes

PubMed Central

Tibirica, Eduardo; Rodrigues, Elba; Cobas, Roberta; Gomes, Marilia B.

2007-01-01

Microvascular function in patients with type 1 diabetes without chronic complications was assessed using skin capillary recruitment during post-occlusive reactive hyperemia (PORH). Structural (maximal) capillary density was evaluated during venous occlusion. The study included 48 consecutive outpatients aged 26.3 ± 10.8 years with type 1 diabetes (duration of 9.5 years) without chronic complications and 34 control subjects. Intravital capillary video-microscopy was used in the dynamic study of skin capillaries in the dorsum of the fingers and toes. Capillary recruitment during PORH (% increase in mean capillary density, MCD) was significantly higher in the controls than the patients in both the fingers (p < 0.001) and toes (p < 0.001). During venous occlusion, MCD increase was also higher in the controls than the patients in both the fingers (p < 0.05) and toes (p < 0.0001). In patients, no difference was found between MCD at baseline and after venous occlusion in the fingers but a decrease was observed in the toes (p < 0.001). It is concluded that skin capillary function is significantly impaired in both fingers and toes of patients with type 1 diabetes without chronic complications. Moreover, capillary density during venous occlusion did not increase in either extremity in the patients, suggesting that their capillaries at rest are already maximally recruited. PMID:17823692

[Key factors in the control of electroosmosis with external radial electric field in CE].

PubMed

Zhu, Y; Chen, Y

1999-11-01

Direct control of electroosmosis flow (EOF) by external radial electric field was performed at room temperature using a home-made field-modulated capillary electrophoresis (CE) system. The EOF was monitored at 206 nm by using DMSO as a probe. To apply a radial electric field across the CE capillary wall, the capillary was cased with a wide column. Both of the concentric space and the capillary bore were then filled with an identical running buffer and applied with an axial electric field of 150 V/cm but starting from different levels. All of the tubes used were made of fused silica with polyimide over-coating (from the Yongnian Optical Fiber Work, Hebei, P. R. China). The size of the CE capillaries adopted was 25-100 microns i.d. (375 microns o.d.) x 28.5/45 cm (effective/total length), and that of the casing column 400 microns i.d. x 32 cm. To investigate the fundamentals of the external EOF control when using the flexible fused silica capillaries, various parameters have been inspected such as pH, buffer composition, additives and capillary wall feature etc.. As expected, to well control both of the magnitude and direction of the electroosmosis, the buffer pH should be kept below 4 and the buffer concentration below 50 mmol/L. However, buffers below 1 mmol/L should be avoided because such a diluted running buffer may result in poor CE separation. Weak electrolytes like citric acid, tartaric acid and acetic acid were found to be capable of generating better EOF control than the strong electrolytes such as phosphate and chlorides. This is possibly due to the formation of looser electric double layer with the weak rather than the strong electrolytes. Some wall coatings like calix arene and its derivatives can evidently improve the EOF control even at pH 5. This reveals an exciting way to expend the controllable pH range. In addition, narrow-bore capillaries were demonstrated to be better than wide-bore tubes. Other conditions such as buffer additives and capillary rinse procedure were shown to have only negligible influence on the control.

Subsurface Flow and Moisture Dynamics in Response to Swash Motions: Effects of Beach Hydraulic Conductivity and Capillarity

NASA Astrophysics Data System (ADS)

Geng, Xiaolong; Heiss, James W.; Michael, Holly A.; Boufadel, Michel C.

2017-12-01

A combined field and numerical study was conducted to investigate dynamics of subsurface flow and moisture response to waves in the swash zone of a sandy beach located on Cape Henlopen, DE. A density-dependent variably saturated flow model MARUN was used to simulate subsurface flow beneath the swash zone. Values of hydraulic conductivity (K) and characteristic pore size (α, a capillary fringe property) were varied to evaluate their effects on subsurface flow and moisture dynamics in response to swash motions in beach aquifers. The site-specific modeling results were validated against spatiotemporal measurements of moisture and pore pressure in the beach. Sensitivity analyses indicated that the hydraulic conductivity and capillary fringe thickness of the beach greatly influenced groundwater flow pathways and associated transit times in the swash zone. A higher value of K enhanced swash-induced seawater infiltration into the beach, thereby resulting in a faster expansion of a wedge of high moisture content induced by swash cycles, and a flatter water table mound beneath the swash zone. In contrast, a thicker capillary fringe retained higher moisture content near the beach surface, and thus, significantly reduced the available pore space for infiltration of seawater. This attenuated wave effects on pore water flow in the unsaturated zone of the beach. Also, a thicker capillary fringe enhanced horizontal flow driven by the larger-scale hydraulic gradient caused by tides.

Current characteristics of λ-DNA molecules/polystyrene nanoparticles in TBE buffer solution through micro/nanofluidic capillaries under DC electric field

NASA Astrophysics Data System (ADS)

Duan, Yifei; Zhao, Wei; Xue, Jing; Sun, Dan; Wang, Kaige; Wang, Guiren; Li, Junjie; Bai, Jintao; Gu, Changzhi

2017-03-01

In practical applications of biochips and bio-sensors, electrokinetic mechanisms are commonly employed to manipulate single bio-molecules and analyze their characteristics. To accurately and flexibly control the movement of single-molecule within micro/nanofluidic channels which are the basic components of Lab-chips, the current signals in micro/nanocapillaries filled with solutions of DNA molecules or polystyrene (PS) nanoparticles are systematically studied. Experimental results indicate that the current response along the micro/nanocapillaries can be significantly influenced by the diameter of the capillaries and the pH value of the solutions. Specifically, when there is only a pure (TBE) solution, the electric conductance does not monotonically decrease with decreasing the diameter of the capillaries, but slightly increases with decreasing the capillary diameter. When λ-DNA molecules or PS nanoparticles are added into the TBE buffer, the size effect on the electric conductance of the solutions are quite different. Although in the former, the electric conductance behaves differently from that in the pure TBE solution and decreases with the decreasing diameter, in the latter, the change is similar to that in the pure TBE solution. Besides, an abnormal ‘falling’ of the electric conductance is observed in a capillary with diameter of 200 nm. The investigation will significantly enhance the understanding on the electric properties of the solutions of biomolecules and particles in micro/nanofluidics. This is especially helpful for designing functional Lab-chip devices.

Behavior of CO2/water flow in porous media for CO2 geological storage.

PubMed

Jiang, Lanlan; Yu, Minghao; Liu, Yu; Yang, Mingjun; Zhang, Yi; Xue, Ziqiu; Suekane, Tetsuya; Song, Yongchen

2017-04-01

A clear understanding of two-phase fluid flow properties in porous media is of importance to CO 2 geological storage. The study visually measured the immiscible and miscible displacement of water by CO 2 using MRI (magnetic resonance imaging), and investigated the factor influencing the displacement process in porous media which were filled with quartz glass beads. For immiscible displacement at slow flow rates, the MR signal intensity of images increased because of CO 2 dissolution; before the dissolution phenomenon became inconspicuous at flow rate of 0.8mLmin -1 . For miscible displacement, the MR signal intensity decreased gradually independent of flow rates, because supercritical CO 2 and water became miscible in the beginning of CO 2 injection. CO 2 channeling or fingering phenomena were more obviously observed with lower permeable porous media. Capillary force decreases with increasing particle size, which would increase permeability and allow CO 2 and water to invade into small pore spaces more easily. The study also showed CO 2 flow patterns were dominated by dimensionless capillary number, changing from capillary finger to stable flow. The relative permeability curve was calculated using Brooks-Corey model, while the results showed the relative permeability of CO 2 slightly decreases with the increase of capillary number. Copyright © 2016 Elsevier Inc. All rights reserved.

Aquaporin-4 Functionality and Virchow-Robin Space Water Dynamics: Physiological Model for Neurovascular Coupling and Glymphatic Flow

PubMed Central

Kwee, Ingrid L.

2017-01-01

The unique properties of brain capillary endothelium, critical in maintaining the blood-brain barrier (BBB) and restricting water permeability across the BBB, have important consequences on fluid hydrodynamics inside the BBB hereto inadequately recognized. Recent studies indicate that the mechanisms underlying brain water dynamics are distinct from systemic tissue water dynamics. Hydrostatic pressure created by the systolic force of the heart, essential for interstitial circulation and lymphatic flow in systemic circulation, is effectively impeded from propagating into the interstitial fluid inside the BBB by the tightly sealed endothelium of brain capillaries. Instead, fluid dynamics inside the BBB is realized by aquaporin-4 (AQP-4), the water channel that connects astrocyte cytoplasm and extracellular (interstitial) fluid. Brain interstitial fluid dynamics, and therefore AQP-4, are now recognized as essential for two unique functions, namely, neurovascular coupling and glymphatic flow, the brain equivalent of systemic lymphatics. PMID:28820467

Aquaporin-4 Functionality and Virchow-Robin Space Water Dynamics: Physiological Model for Neurovascular Coupling and Glymphatic Flow.

PubMed

Nakada, Tsutomu; Kwee, Ingrid L; Igarashi, Hironaka; Suzuki, Yuji

2017-08-18

The unique properties of brain capillary endothelium, critical in maintaining the blood-brain barrier (BBB) and restricting water permeability across the BBB, have important consequences on fluid hydrodynamics inside the BBB hereto inadequately recognized. Recent studies indicate that the mechanisms underlying brain water dynamics are distinct from systemic tissue water dynamics. Hydrostatic pressure created by the systolic force of the heart, essential for interstitial circulation and lymphatic flow in systemic circulation, is effectively impeded from propagating into the interstitial fluid inside the BBB by the tightly sealed endothelium of brain capillaries. Instead, fluid dynamics inside the BBB is realized by aquaporin-4 (AQP-4), the water channel that connects astrocyte cytoplasm and extracellular (interstitial) fluid. Brain interstitial fluid dynamics, and therefore AQP-4, are now recognized as essential for two unique functions, namely, neurovascular coupling and glymphatic flow, the brain equivalent of systemic lymphatics.

Micro-channel filling flow considering surface tension effect

NASA Astrophysics Data System (ADS)

Kim, Dong Sung; Lee, Kwang-Cheol; Kwon, Tai Hun; Lee, Seung S.

2002-05-01

Understanding filling flow into micro-channels is important in designing micro-injection molding, micro-fluidic devices and an MIMIC (micromolding in capillaries) process. In this paper, we investigated, both experimentally and numerically, 'transient filling' flow into micro-channels, which differs from steady-state completely 'filled' flow in micro-channels. An experimental flow visualization system was devised to facilitate observation of flow characteristics in filling into micro-channels. Three sets of micro-channels of various widths of different thicknesses (20, 30, and 40 μm) were fabricated using SU-8 on the silicon substrate to find a geometric effect with regard to pressure gradient, viscous force and, in particular, surface tension. A numerical analysis system has also been developed taking into account the surface tension effect with a contact angle concept. Experimental observations indicate that surface tension significantly affects the filling flow to such an extent that even a flow blockage phenomenon was observed at channels of small width and thickness. A numerical analysis system also confirms that the flow blockage phenomenon could take place due to the flow hindrance effect of surface tension, which is consistent with experimental observation. For proper numerical simulations, two correction factors have also been proposed to correct the conventional hydraulic radius for the filling flow in rectangular cross-sectioned channels.

Investigation of water droplet dynamics in PEM fuel cell gas channels

NASA Astrophysics Data System (ADS)

Gopalan, Preethi

Water management in Proton Exchange Membrane Fuel Cell (PEMFC) has remained one of the most important issues that need to be addressed before its commercialization in automotive applications. Accumulation of water on the gas diffusion layer (GDL) surface in a PEMFC introduces a barrier for transport of reactant gases through the GDL to the catalyst layer. Despite the fact that the channel geometry is one of the key design parameters of a fluidic system, very limited research is available to study the effect of microchannel geometry on the two-phase flow structure. In this study, the droplet-wall dynamics and two-phase pressure drop across the water droplet present in a typical PEMFC channel, were examined in auto-competitive gas channel designs (0.4 x 0.7 mm channel cross section). The liquid water flow pattern inside the gas channel was analyzed for different air velocities. Experimental data was analyzed using the Concus-Finn condition to determine the wettability characteristics in the corner region. It was confirmed that the channel angle along with the air velocity and the channel material influences the water distribution and holdup within the channel. Dynamic contact angle emerged as an important parameter in controlling the droplet-wall interaction. Experiments were also performed to understand how the inlet location of the liquid droplet on the GDL surface affects the droplet dynamic behavior in the system. It was found that droplets emerging near the channel wall or under the land lead to corner filling of the channel. Improvements in the channel design has been proposed based on the artificial channel roughness created to act as capillary grooves to transport the liquid water away from the land area. For droplets emerging near the center of the channel, beside the filling and no-filling behavior reported in the literature, a new droplet jumping behavior was observed. As droplets grew and touched the sidewalls, they jumped off to the sidewall leaving the whole GDL exposed for gases to diffuse to the catalyst layer. A theoretical model was developed and a criterion was proposed to predict the droplet jumping behavior in the gas channel. A theoretical force balance model was proposed to predict the pressure force and air velocity required to remove the droplet from the channel to avoid complete channel blockage. The overall goal of this work was to identify the gas channel configuration that provides efficient water removal with a lower pressure drop in the system efficiency while meeting the US Department of Energy's specifications for a PEMFC for automotive application.

A Capillary Flow Dynamics-Based Sensing Modality for Direct Environmental Pathogen Monitoring.

PubMed

Klug, Katherine E; Reynolds, Kelly A; Yoon, Jeong-Yeol

2018-04-20

Toward ultra-simple and field-ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody-conjugated submicron particle suspensions. Differential capillary flow is tunable, allowing pathogen quantification as a function of flow rate through a paper-based microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target-particle aggregation. This mechanism is demonstrated for assays of Escherichia coli K12 in water samples and Zika virus (ZIKV) in blood serum. These assays achieved very low limits of detection compared with other demonstrated methods (1 log CFU/mL E. coli and 20 pg/mL ZIKV whole virus) with an operating time of 30 s, showing promise for environmental and health monitoring. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Sol-gel chemistry-based Ucon-coated columns for capillary electrophoresis.

PubMed

Hayes, J D; Malik, A

1997-07-18

A sol-gel chemistry-based novel approach for the preparation of a Ucon-coated fused-silica capillary column in capillary electrophoresis is presented. In this approach the sol-gel process is carried out inside 25 microm I.D. fused-silica capillaries. The sol solution contained appropriate quantities of an alkoxide-based sol-gel precursor, a polymeric coating material (Ucon), a crosslinking reagent, a surface derivatizing reagent, controlled amounts of water and a catalyst dissolved in a suitable solvent system. The coating procedure involves filling a capillary with the sol solution and allowing the sol-gel process to proceed for an optimum period. Hydrolysis of the alkoxide precursor and polycondensation of the hydrolyzed products with the surface silanol groups and the hydroxy-terminated Ucon molecules lead to the formation of a surface-bonded sol-gel coating on the inner walls of the capillary. The thickness of the coated film can be controlled by varying the reaction time, coating solution composition and experimental conditions. Commercial availability of high purity sol-gel precursors (e.g., TEOS 99.999%), the ease of coating, run-to-run and column-to-column reproducibility, and long column lifetimes make sol-gel coating chemistry very much suitable for being applied in analytical microseparations column technology. Test samples of basic proteins and nucleotides were used to evaluate the column performance. These results show that the sol-gel coating scheme has allowed for the generation of bio-compatible surfaces characterized by high separation efficiencies in CE. For different types of solutes, the sol-gel coated Ucon column consistently provided migration time R.S.D. values of the order of 0.5%.

Terrestrial Testing of the CapiBRIC, a Microgravity Optimized Brine Processor

NASA Technical Reports Server (NTRS)

Sargusingh, Miriam J.; Callahan, Michael R.; Weislogel, Mark M.

2016-01-01

Utilizing geometry based static phase separation exhibited in the radial vaned capillary drying tray, a system was conceived to recover water from brine. This technology has been named the Capillary BRIC; abbreviated CapiBRIC. The CapiBRIC utilizes a capillary drying tray within a drying chamber. Water is recovered from clean water vapor evaporating from the free surface leaving waste brine solids behind. A novel approach of optimizing the containment geometry to support passive capillary flow and static phase separation provides the opportunity for a low power system that is not as susceptible to fouling as membranes or other technologies employing physical barriers across the free brine surface to achieve phase separation in microgravity. Having been optimized for operation in microgravity, full-scale testing of the CapiBRIC as designed cannot be performed on the ground as the force of gravity would dominate over the capillary forces. However, subscale units relevant to full-scale design were used to characterize fill rates, containment stability, and interaction with a variable volume reservoir in the PSU Dryden Drop Tower (DDT) facility. PSU also using tested units scaled such that capillary forces dominated in a 1-g environment to characterize evaporation from a free-surface in 1-g upward, sideways and downward orientations. In order to augment the subscale testing performed by PSU, a full scale 1-g analogue of the CapiBRIC drying unit was initiated to help validate performance predictions regarding expected water recovery ratio, estimated processing time, and interface definitions for inlets, outlets, and internal processes, including vent gas composition. This paper describes the design, development and test of the terrestrial CapiBRIC prototypes.

Continuous condensation in nanogrooves

NASA Astrophysics Data System (ADS)

Malijevský, Alexandr

2018-05-01

We consider condensation in a capillary groove of width L and depth D , formed by walls that are completely wet (contact angle θ =0 ), which is in a contact with a gas reservoir of the chemical potential μ . On a mesoscopic level, the condensation process can be described in terms of the midpoint height ℓ of a meniscus formed at the liquid-gas interface. For macroscopically deep grooves (D →∞ ), and in the presence of long-range (dispersion) forces, the condensation corresponds to a second-order phase transition, such that ℓ ˜(μcc-μ ) -1 /4 as μ →μcc - where μc c is the chemical potential pertinent to capillary condensation in a slit pore of width L . For finite values of D , the transition becomes rounded and the groove becomes filled with liquid at a chemical potential higher than μc c with a difference of the order of D-3. For sufficiently deep grooves, the meniscus growth initially follows the power law ℓ ˜(μcc-μ ) -1 /4 , but this behavior eventually crosses over to ℓ ˜D -(μ-μc c) -1 /3 above μc c, with a gap between the two regimes shown to be δ ¯μ ˜D-3 . Right at μ =μc c , when the groove is only partially filled with liquid, the height of the meniscus scales as ℓ*˜(D3L) 1 /4 . Moreover, the chemical potential (or pressure) at which the groove is half-filled with liquid exhibits a nonmonotonic dependence on D with a maximum at D ≈3 L /2 and coincides with μc c when L ≈D . Finally, we show that condensation in finite grooves can be mapped on the condensation in capillary slits formed by two asymmetric (competing) walls a distance D apart with potential strengths depending on L . All these predictions, based on mesoscopic arguments, are confirmed by fully microscopic Rosenfeld's density functional theory with a reasonable agreement down to surprisingly small values of both L and D .

Chitosan as cationic polyelectrolyte for the modification of electroosmotic flow and its utilization for the separation of inorganic anions by capillary zone electrophoresis.

PubMed

Takayanagi, Toshio; Motomizu, Shoji

2006-09-01

Cationic polyelectrolyte of chitosan was used for the reversal of electroosmotic flow in capillary zone electrophoresis. The chitosan was dissolved in acetic acid solution, and stable electroosmotic flow was obtained at the chitosan concentrations between 50 and 300 microg/mL. Separation of inorganic anions was carried out using the dynamically coated capillary by capillary zone electrophoresis. Nine kinds of anions were separated and detected with the capillary. The electrophoretic mobility of the analyte anions decreased with increasing concentrations of chitosan in the migrating solution through ion-ion interaction, but the migration order of the analyte anions was not changed in the concentration range of the chitosan examined. The signal shape for the analyte anions was developed by using field-enhanced sample stacking with 10 mM sodium sulfate.

Acoustic cavitation of individual ultrasound contrast agent microbubbles confined in capillaries

NASA Astrophysics Data System (ADS)

Almaqwashi, Ali; McIntyre, David; Ammi, Azzdine

2011-10-01

Ultrasound targeted therapies mainly rely on the inertial cavitation of ultrasound contrast agent (UCA) microbubbles. Our objective is to determine the cavitation acoustic pressure threshold for the destruction of UCA microbubbles inside cellulose capillaries. Acoustic emission from individual Optison microbubbles confined inside a 200-μm diameter capillary was detected using a passive cavitation detection system. Excitation signals from a 2.25 MHz transmitter were applied to the microbubbles while their acoustic emission was detected by a broadband 15 MHz receiver. Time traces were recorded (100 MHz sampling, 12- bit), and frequency-domain analysis of the received signals was performed to characterize microbubble cavitation. The cavitation acoustic pressure threshold was found to be 1 MPa inside the capillary in comparison with ˜0.7 MPa previously reported for unconfined UCA microbubbles. This work provides a clearer understanding of the role of ultrasound contrast agent dynamics inside a capillary.

Computer capillaroscopy as a new cardiological diagnostics method

NASA Astrophysics Data System (ADS)

Gurfinkel, Youri I.; Korol, Oleg A.; Kufal, George E.

1998-04-01

The blood flow in capillary vessels plays an important role in sustaining the vital activity of the human organism. The computerized capillaroscope is used for the investigations of nailfold (eponychium) capillary blood flow. An important advantage of the instrument is the possibility of performing non-invasive investigations, i.e., without damage to skin or vessels and causing no pain or unpleasant sensations. The high-class equipment and software allow direct observation of capillary blood flow dynamics on a computer screen at a 700 - 1300 times magnification. For the first time in the clinical practice, it has become possible to precisely measure the speed of capillary blood flow, as well as the frequency of aggregate formation (glued together in clots of blood particles). In addition, provision is made for automatic measurement of capillary size and wall thickness and automatic recording of blood aggregate images for further visual study, documentation, and electronic database management.

Multicomponent micropatterned sol-gel materials by capillary molding

NASA Astrophysics Data System (ADS)

Lochhead, Michael J.; Yager, Paul

1997-10-01

A physically and chemically benign method for patterning multiple sol-gel materials onto a single substrate is described. Structures are demonstrated for potential micro- optical chemical sensor, biosensor, and waveguiding applications. Fabrication is based on the micro molding in capillaries (MIMIC) approach. A novel mold design allows several sols to be cast simultaneously. Closely spaced, organically modified silica ridges containing fluorescent dyes are demonstrated. Ridges have cross sectional dimensions from one to 50 micrometers and are centimeters in length. Processing issues, particularly those related to mold filling, are discussed in detail. Because sol-gel MIMIC avoids the harsh physical and chemical environments normally associated with patterning, the approach allows full exploitation of sol- gel processing advantages, such as the ability to entrap sensitive organic dopant molecules in the sol-gel matrix.

Copolymers For Capillary Gel Electrophoresis

DOEpatents

Liu, Changsheng; Li, Qingbo

2005-08-09

This invention relates to an electrophoresis separation medium having a gel matrix of at least one random, linear copolymer comprising a primary comonomer and at least one secondary comonomer, wherein the comonomers are randomly distributed along the copolymer chain. The primary comonomer is an acrylamide or an acrylamide derivative that provides the primary physical, chemical, and sieving properties of the gel matrix. The at least one secondary comonomer imparts an inherent physical, chemical, or sieving property to the copolymer chain. The primary and secondary comonomers are present in a ratio sufficient to induce desired properties that optimize electrophoresis performance. The invention also relates to a method of separating a mixture of biological molecules using this gel matrix, a method of preparing the novel electrophoresis separation medium, and a capillary tube filled with the electrophoresis separation medium.

Velocity dependence of biphasic flow structuration: steady-state and oscillating flow effects

NASA Astrophysics Data System (ADS)

Tore Tallakstad, Ken; Jankov, Mihailo; Løvoll, Grunde; Toussaint, Renaud; Jørgen Mâløy, Knut; Grude Flekkøy, Eirik; Schmittbuhl, Jean; Schäfer, Gerhard; Méheust, Yves; Arendt Knudsen, Henning

2010-05-01

We study various types of biphasic flows in quasi-two-dimensional transparent porous models. These flows imply a viscous wetting fluid, and a lowly viscous one. The models are transparent, allowing the displacement process and structure to be monitored in space and time. Three different aspects will be presented: 1. In stationary biphasic flows, we study the relationship between the macroscopic pressure drop (related to relative permeability) and the average flow rate, and how this arises from the cluster size distribution of the lowly viscous fluid [1]. 2. In drainage situations, we study how the geometry of the invader can be explained, and how it gives rise to apparent dynamic capillary effects. We show how these can be explained by viscous effects on evolving geometries of invading fluid [2]. 3. We study the impact of oscillating pressure fields superimposed to a background flow over the flow regimes patterns [3]. Steady-State Two-Phase Flow in Porous Media: Statistics and Transport Properties. First, in stationary flow with a control of the flux of both fluids, we show how the pressure drop depends on the flow rate. We will show that the dynamics is dominated by the interplay between a viscous pressure field from the wetting fluid and bubble transport of a less viscous, nonwetting phase. In contrast with more studied displacement front systems, steady-state flow is in equilibrium, statistically speaking. The corresponding theoretical simplicity allows us to explain a data collapse in the cluster size distribution of lowly viscous fluid in the system, as well as the relation |?P|∞√Ca--. This allows to explain so called relative permeability effects by the morphological changes of the cluster size distribution. Influence of viscous fingering on dynamic saturation-pressure curves in porous media. Next, we study drainage in such models, and investigate the relationship between the pressure field and the morphology of the invading fluid. This allows to model the impact of the saturation changes in the system over the pressure difference between the wetting and non wetting phase. We show that the so-called dynamic effects referred in the hydrology literature of experimentally measured capillary pressure curves might be explained by the combined effect of capillary pressure along the invasion front of the gaseous phase and pressure changes caused by viscous effects. A detailed study of the structure optically followed shows that the geometry of the invader is self-similar with two different behaviors at small and large scales: the structure corresponds to the ones of invasion percolation models at small scales (capillary fingering structures with fractal dimension D=1.83), whereas at large scales, viscous pressure drops dominate over the capillary threshold variations, and the structures are self-similar fingering structures with a fractal dimension corresponding to Dielectric Breakdown Models (variants of the DLA model), with D ≠ƒ 1.5. The cross-over scale is set by the scale at which capillary fluctuations are of the order of the viscous pressure drops. This leads physically to the fact that cross-over scale between the two fingering dimensions, goes like the inverse of the capillary number. This study utilizes these geometrical characteristics of the viscous fingers forming in dynamic drainage, to obtain a meaningfull scaling law for the saturation-pressure curve at finite speed, i.e. the so-called dynamic capillary pressure relations. We thus show how the micromechanical interplay between viscous and capillary forces leads to some pattern formation, which results in a general form of dynamic capillary pressure relations. By combining these detailed informations on the displacement structure with global measures of pressure, saturation and controlling the capillary number Ca, a scaling relation relating pressure, saturation, system size and capillary number is developed. By applying this scaling relation, pressure-saturation curves for a wide range of capillary numbers can be collapsed. Effects of pressure oscillations on drainage in an elastic porous medium: The effects of seismic stimulation on the flow of two immiscible fluids in an elastic synthetic porous medium is experimentally investigated. A wetting fluid is slowly evacuated from the medium, while a pressure oscillation is applied on the injected non-wetting fluid. The amplitude and frequency of the pressure oscillations as well as the evacuation speed are kept constant throughout an experiment. The resulting morphology of the invading structure is found to be strongly dependent on the interplay between the amplitude and the frequency of the applied pressure oscillations and the elasticity of the porous medium. Different combinations of these properties yield morphologically similar structures, allowing a classification of structures that is found to depend on a proposed dimensionless number. [1] Tallakstad, K.T., H.A. Knudsen, T. Ramstad, G. Løvoll, K.J. Maløy, R. Toussaint and E.G. Flekkøy , Steady-state two-phase flow in porous media: statistics and transport properties, Phys. Rev. Lett. 102, 074502 (2009). doi:10.1103/PhysRevLett.102.074502 [2] Løvoll, G., M. Jankov, K.J. Maløy, R. Toussaint, J. Schmittbuhl, G. Schaefer and Y. Ḿ eheust, Influence of viscous fingering on dynamic saturation-pressure curves in porous media, submitted to Transport In Porous Media, (2010) [3] Jankov, M., G. Løvoll, H.A. Knudsen, K.J. Maløy, R. Planet, R. Toussaint and E.G. Flekkøy; Effects of pressure oscillations on drainage in an elastic porous medium, Transport In Porous Media, in press (2010).

Fluid Dynamics of Bottle Filling

NASA Astrophysics Data System (ADS)

McGough, Patrick; Gao, Haijing; Appathurai, Santosh; Basaran, Osman

2011-11-01

Filling of bottles is a widely practiced operation in a large number of industries. Well known examples include filling of ``large'' bottles with shampoos and cleaners in the household products and beauty care industries and filling of ``small'' bottles in the pharmaceutical industry. Some bottle filling operations have recently drawn much attention from the fluid mechanics community because of the occurrence of a multitude of complex flow regimes, transitions, and instabilities such as mounding and coiling that occur as a bottle is filled with a fluid. In this talk, we present a primarily computational study of the fluid dynamical challenges that can arise during the rapid filling of bottles. Given the diversity of fluids used in filling applications, we consider four representative classes of fluids that exhibit Newtonian, shear-thinning, viscoelastic, and yield-stress rheologies. The equations governing the dynamics of bottle filling are solved either in their full 3D but axisymmetric form or using the slender-jet approximation.

Critical capillary channel flow

NASA Astrophysics Data System (ADS)

Grah, Aleksander; Klatte, Jörg; Dreyer, Michael E.

The main subject are numerical studies on capillary channel flow, based on results of the sounding rocket experiments TEXUS 41/42. The flow through a capillary channel is established by a gear pump at the outlet. The channel, consists of two parallel glass plates with a width of 25 mm, a gap of 10 mm and a length of 12 mm. The meniscus of a compensation tube maintains a constant system pressure. Steady and dynamic pressure effects in the system force the surfaces to bend inwards. A maximum flow rate is achieved when the free surface collapses and gas ingestion occurs at the outlet. This critical flow rate depends on the channel geometry, the flow regime and the liquid properties. The aim of the experiments is the determination of the free surface shape and to find the maximum flow rate. In order to study the unsteady liquid loop behaviour, a dimensionless transient model was developed. It is based on the unsteady Bernoulli equation, the unsteady continuity equation and geometrical conditions for the surface curvature and the flow cross-section. The pressure is related to the curvature of the free liquid surface by the dimensionless Gauss-Laplace equation with two principal radii. The experimental and evaluated contour data shows good agreement for a sequence of transient flow rate perturbations. The surface oscillation frequencies and amplitudes can be predicted with quite high accuracy. The dynamic of the pump is defined by the increase of the flow rate in a time period. To study the unsteady system behavior in the "worst case", we use a perturbations related to the natural frequency of the oscillating liquid. In the case of steady flow at maximum flow rate, when the "choking" effect occurs, the surfaces collapse and cause gas ingestion into the channel. This effect is related to the Speed Index. At the critical flow rate the Speed Index reaches the value Sca = 1, in analogy to the Mach Number. Unsteady choking does not necessarily cause surface collapse. We show, that temporarily Speed Index values exceeding One may be achieved for a perfectly stable supercritical dynamic flow. As a supercritical criterion for the dynamic free surface stability we define a Dynamic Index D considering the local capillary pressure and the convective pressure, which is a function of the local velocity. The Dynamic Index is below One for stable flow while D = 1 indicates surface collapse. This studies result in a stability diagram, which defines the limits of flow dynamics and the maximum unsteady flow rate. It may serve as a road map for open capillary channel flow control.

Drop Tower Experiments concerning Fluid Management under Microgravity

NASA Astrophysics Data System (ADS)

Gaulke, Diana; Dreyer, Michael

2012-07-01

Transport and positioning of liquid under microgravity is done utilizing capillary forces. Therefore, capillary transport processes have to be understood for a wide variety of space applications, ranging from propellant management in tanks of space transportation systems to eating and drinking devices for astronauts. There are two types of liquid transportation in microgravity using capillary forces. First, the driven liquid flow in open channels where the capillary forces at free surfaces ensure a gas and vapor free flow. Here it is important to know the limiting flow rate through such an open channel before the free surface collapses and gas is sucked into the channel. A number of different experiments at the drop tower Bremen, on sounding rockets and at the ISS have been conducted to analyse this phenomenon within different geometries. As result a geometry dependent theory for calculating the maximum flow rate has been found. On the other hand liquid positioning and transportation requires the capillary pressure of curved surfaces to achieve a liquid flow to a desired area. Especially for space applications the weight of structure has to be taken into account for development. For example liquid positioning in tanks can be achieved via a complicated set of structure filling the whole tank resulting in heavy devices not reasonable in space applications. Astrium developed in cooperation with ZARM a propellant management device much smaller than the tank volume and ensuring a gas and vapour free supply of propellant to the propulsion system. In the drop tower Bremen a model of this device was tested concerning different microgravity scenarios. To further decrease weight and ensure functionality within different scenarios structure elements are designed as perforated geometries. Capillary transport between perforated plates has been analyzed concerning the influence of geometrical pattern of perforations. The conducted experiments at the drop tower Bremen show the remarkable influence of perforations on the capillary transport capability.

Analysis of microdialysate monoamines, including noradrenaline, dopamine and serotonin, using capillary ultra-high performance liquid chromatography and electrochemical detection.

PubMed

Ferry, Barbara; Gifu, Elena-Patricia; Sandu, Ioana; Denoroy, Luc; Parrot, Sandrine

2014-03-01

Electrochemical methods are very often used to detect catecholamine and indolamine neurotransmitters separated by conventional reverse-phase high performance liquid chromatography (HPLC). The present paper presents the development of a chromatographic method to detect monoamines present in low-volume brain dialysis samples using a capillary column filled with sub-2μm particles. Several parameters (repeatability, linearity, accuracy, limit of detection) for this new ultrahigh performance liquid chromatography (UHPLC) method with electrochemical detection were examined after optimization of the analytical conditions. Noradrenaline, adrenaline, serotonin, dopamine and its metabolite 3-methoxytyramine were separated in 1μL of injected sample volume; they were detected above concentrations of 0.5-1nmol/L, with 2.1-9.5% accuracy and intra-assay repeatability equal to or less than 6%. The final method was applied to very low volume dialysates from rat brain containing monoamine traces. The study demonstrates that capillary UHPLC with electrochemical detection is suitable for monitoring dialysate monoamines collected at high sampling rate. Copyright © 2014 Elsevier B.V. All rights reserved.

Squeezing and de-wetting of a shear thinning fluid drop between plane parallel surfaces: capillary adhesion phenomenon

NASA Astrophysics Data System (ADS)

Ward, Thomas

2017-11-01

The radial squeezing and de-wetting of a thin film of viscous shear thinning fluid filling the gap between parallel plane walls is examined both experimentally and theoretically for gap spacing much smaller than the capillary length. The interaction between motion of fluid in the gap driven by squeezing or de-wetting and surface tension is parameterized by a dimensionless variable, F, that is the ratio of the constant force supplied by the top plate (either positive or negative) to surface tension at the drop's circumference. Furthermore, the dimensionless form of the rate equation for the gap's motion reveals a time scale that is dependent on the drop volume when analyzed for a power law shear thinning fluid. In the de-wetting problem the analytical solution reveals the formation of a singularity, leading to capillary adhesion, as the gap spacing approaches a critical value that depends on F and the contact angle. Experiments are performed to test the analytical predictions for both squeezing, and de-wetting in the vicinity of the singularity.

Microjet formation in a capillary by laser-induced cavitation

NASA Astrophysics Data System (ADS)

Peters, Ivo R.; Tagawa, Yoshiyuki; van der Meer, Devaraj; Prosperetti, Andrea; Sun, Chao; Lohse, Detlef

2010-11-01

A vapor bubble is created by focusing a laser pulse inside a capillary that is partially filled with water. Upon creation of the bubble, a shock wave travels through the capillary. When this shock wave meets the meniscus of the air-water interface, a thin jet is created that travels at very high speeds. A crucial ingredient for the creation of the jet is the shape of the meniscus, which is responsible for focusing the energy provided by the shock wave. We examine the formation of this jet numerically using a boundary integral method, where we prepare an initial interface at rest inside a tube with a diameter ranging from 50 to 500 μm. To simulate the effect of the bubble we then apply a short, strong pressure pulse, after which the jet forms. We investigate the influence of the shape of the meniscus, and pressure amplitude and duration on the jet formation. The jet shape and velocity obtained by the simulation compare well with experimental data, and provides good insight in the origin of the jet.

Microbial growth and transport in saturated and unsaturated porous media

NASA Astrophysics Data System (ADS)

Hron, Pavel; Jost, Daniel; Bastian, Peter; Ippisch, Olaf

2014-05-01

There is a considerable ongoing effort aimed at understanding the behavior of microorganisms in porous media. Microbial activity is of significant interest in various environmental applications such as in situ bioremediation, protection of drinking water supplies and for subsurface geochemistry in general. The main limiting factors for bacterial growth are the availability of electron acceptors, nutrients and bio-available water. The capillary fringe, defined - in a wider sense than usual - as the region of the subsurface above the groundwater table, but still dominated by capillary rise, is a region where all these factors are abundantly available. It is thus a region where high microbial activity is to be expected. In a research unit 'Dynamic Capillary Fringes - A Multidisciplinary Approach (DyCap)' founded by the German Research Foundation (DFG), the growth of microorganisms in the capillary fringe was studied experimentally and with numerical simulations. Processes like component transport and diffusion, exchange between the liquid phase and the gas phase, microbial growth and cell attachment and detachment were incorporated into a numerical simulator. The growth of the facultative anaerobic Escherichia coli as a function of nutrient availability and oxygen concentration in the liquid phase is modeled with modified Monod-type models and modifications for the switch between aerobic and anaerobic growth. Laboratory batch experiments with aqueous solutions of bacteria have been carried out under various combinations of oxygen concentrations in the gas phase and added amounts of dissolved organic carbon to determine the growth model parameters by solution of a parameter estimation problem. For the transport of bacteria the adhesion to phase boundaries is also very important. As microorganisms are transported through porous media, they are removed from the pore fluid by physicochemical filtration (attachment to sediment grain surfaces) or are adhering to gas-water interface. The cell attachment and detachment model was based on flow-through experiments and the parameters were obtained by fitting the model to measured bacteria breakthrough curves. Experiments on bacterial growth in porous media with and without groundwater flow were performed in Hele-Shaw cells filled with quartz sands. The cell density was determined by the fluorescence of a special protein produced by the genetically modified strain of E. coli. The simulation results are compared to experimental data and different modeling approaches are discussed.

Capillary waves' dynamics at the nanoscale

NASA Astrophysics Data System (ADS)

Delgado-Buscalioni, Rafael; Chacón, Enrique; Tarazona, Pedro

2008-12-01

We study the dynamics of thermally excited capillary waves (CW) at molecular scales, using molecular dynamics simulations of simple liquid slabs. The analysis is based on the Fourier modes of the liquid surface, constructed via the intrinsic sampling method (Chacón and Tarazona 2003 Phys. Rev. Lett. 91 166103). We obtain the time autocorrelation of the Fourier modes to get the frequency and damping rate Γd(q) of each mode, with wavenumber q. Continuum hydrodynamics predicts \\Gamma (q) \\propto q\\gamma (q) and thus provides a dynamic measure of the q-dependent surface tension, γd(q). The dynamical estimation is much more robust than the structural prediction based on the amplitude of the Fourier mode, γs(q). Using the optimal estimation of the intrinsic surface, we obtain quantitative agreement between the structural and dynamic pictures. Quite surprisingly, the hydrodynamic prediction for CW remains valid up to wavelengths of about four molecular diameters. Surface tension hydrodynamics break down at shorter scales, whereby a transition to a molecular diffusion regime is observed.

Nonlinear Dynamics of Formation of Drops of Non-Newtonian Liquids from Capillaries: Satellite Formation and Flow Transitions

NASA Astrophysics Data System (ADS)

Yildirim, Ozgur E.; Basaran, Osman A.

1999-11-01

Drop formation from capillaries, and the often undesired phenomenon of satellite generation, play a central role in diverse applications including ink-jet printing, biochip processors, and spray coating, where the working fluid is usually non-Newtonian. Although some work has been done in related areas, the phenomenon of formation of drops of non--Newtonian fluids from capillaries has remained largely unexplored. Here a theoretical approach is adopted to study the dripping of axisymmetric drops of non--Newtonian liquids from capillaries. The constitutive equation used accounts for both shear thinning and strain hardening. First, regular perturbation theory is utilized to reduce the spatial dimension of the governing equations to one. The computations rely on Galerkin/finite element analysis with adaptive finite differencing for time integration. The dynamics are followed beyond the first breakup to investigate conditions for occurrence of satellites. Effect of increasing flow rate is also studied to uncover transitions that occur as one moves from a regime of periodic drop formation to one of jetting.

Capillary electromigration separation of proteins and microorganisms dynamically modified by chromophoric nonionogenic surfactant.

PubMed

Horká, Marie; Růzicka, Filip; Holá, Veronika; Kahle, Vladislav; Moravcová, Dana; Slais, Karel

2009-08-15

A chromophoric nonionogenic surfactant poly(ethylene glycol) 3-(2-hydroxy-5-n-octylphenylazo)-benzoate, HOPAB, has been prepared and used as a buffer additive for a dynamic modification of proteins and/or microorganisms including Escherichia coli , Staphylococcus epidermidis (biofilm-positive and biofilm-negative), and the strains of yeast cells Candida albicans and Candida parapsilosis (biofilm-positive and biofilm-negative) during a capillary electrophoresis and a capillary isoelectric focusing (CIEF) with UV detection at 326 nm. Values of isoelectric points of labeled proteins and microorganisms have been calculated using UV-detectable pI markers and have been found comparable with pI of the native compounds. Minimum detectable amount has been assessed lower than picograms of proteins and lower than a hundred cells injected into a separation capillary. The introduced labeling method facilitates CIEF separation of microorganisms from the clinical sample of the infected urine at their clinically important levels in the pH gradient pH range of 2-5 and their subsequent cultivation. At the same time, it has enabled the determination of albumin in human urine as a major clinical marker of urinary tract infections and kidney diseases.

Superparamagnetic properties of carbon nanotubes filled with NiFe{sub 2}O{sub 4} nanoparticles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stojak Repa, K.; Israel, D.; Phan, M. H., E-mail: phanm@usf.edu, E-mail: sharihar@usf.edu

2015-05-07

Multi walled carbon nanotubes (MWCNTs) were successfully synthesized using custom-made 80 nm pore-size alumina templates, and were uniformly filled with nickel ferrite (NFO) nanoparticles of 7.4 ± 1.7 nm diameter using a novel magnetically assisted capillary action method. X-ray diffraction confirmed the inverse spinel phase for the synthesized NFO. Transmission electron microscopy confirms spherical NFO nanoparticles with an average diameter of 7.4 nm inside MWCNTs. Magnetometry indicates that both NFO and NFO-filled MWCNTs present a blocking temperature around 52 K, with similar superparamagnetic-like behavior, and weak dipolar interactions, giving rise to a super-spin-glass-like behavior at low temperatures. These properties along with the uniformity of sub-100 nm structuresmore » and the possibility of tunable magnetic response in variable diameter carbon nanotubes make them ideal for advanced biomedical and microwave applications.« less

Effect of dispersion forces on the capillary-wave fluctuations of liquid surfaces.

PubMed

Chacón, Enrique; Fernández, Eva M; Tarazona, Pedro

2014-04-01

We present molecular dynamics evidence for the nonanalytic effects of the long-range dispersion forces on the capillary waves fluctuations of a Lennard-Jones liquid surface. The results of the intrinsic sampling method, for the analysis of the instantaneous interfacial shape, are obtained in large systems for several cut-off distances of the potential tail, and they show good agreement with the theoretical prediction by Napiórkowski and Dietrich, based on a density functional analysis. The enhancement of the capillary waves is quantified to be within 1% for a simple liquid near its triple point.

Viscosity of particulate soap films: approaching the jamming of 2D capillary suspensions.

PubMed

Timounay, Yousra; Rouyer, Florence

2017-05-14

We compute the effective viscosity of particulate soap films thanks to local velocity fields obtained by Particle Image Velocimetry (PIV) during film retraction experiments. We identify the jamming of these 2D capillary suspensions at a critical particle surface fraction (≃0.84) where effective viscosity diverges. Pair correlation function and number of neighbors in contact or close to contact reveal the cohesive nature of this 2D capillary granular media. The experimental 2D dynamic viscosities can be predicted by a model considering viscous dissipation at the liquid interfaces induced by the motion of individual particles.

Apparent and microscopic dynamic contact angles in confined flows

NASA Astrophysics Data System (ADS)

Omori, Takeshi; Kajishima, Takeo

2017-11-01

An abundance of empirical correlations between a dynamic contact angle and a capillary number representing a translational velocity of a contact line have been provided for the last decades. The experimentally obtained dynamic contact angles are inevitably apparent contact angles but often undistinguished from microscopic contact angles formed right on the wall. As Bonn et al. ["Wetting and spreading," Rev. Mod. Phys. 81, 739-805 (2009)] pointed out, however, most of the experimental studies simply report values of angles recorded at some length scale which is quantitatively unknown. It is therefore hard to evaluate or judge the physical validity and the generality of the empirical correlations. The present study is an attempt to clear this clutter regarding the dynamic contact angle by measuring both the apparent and the microscopic dynamic contact angles from the identical data sets in a well-controlled manner, by means of numerical simulation. The numerical method was constructed so that it reproduced the fine details of the flow with a moving contact line predicted by molecular dynamics simulations [T. Qian, X. Wang, and P. Sheng, "Molecular hydrodynamics of the moving contact line in two-phase immiscible flows," Commun. Comput. Phys. 1, 1-52 (2006)]. We show that the microscopic contact angle as a function of the capillary number has the same form as Blake's molecular-kinetic model [T. Blake and J. Haynes, "Kinetics of liquid/liquid displacement," J. Colloid Interface Sci. 30, 421-423 (1969)], regardless of the way the flow is driven, the channel width, the mechanical properties of the receding fluid, and the value of the equilibrium contact angle under the conditions where the Reynolds and capillary numbers are small. We have also found that the apparent contact angle obtained by the arc-fitting of the interface behaves surprisingly universally as claimed in experimental studies in the literature [e.g., X. Li et al., "An experimental study on dynamic pore wettability," Chem. Eng. Sci. 104, 988-997 (2013)], although the angle deviates significantly from the microscopic contact angle. It leads to a practically important point that it suffices to measure arc-fitted contact angles to make formulae to predict flow rates in capillary tubes.

Jet dynamics post drop impact on a deep pool

NASA Astrophysics Data System (ADS)

Michon, Guy-Jean; Josserand, Christophe; Séon, Thomas

2017-02-01

We investigate experimentally the jet formed by the collapse of a cavity created by the impact of a drop on a pool of the same aqueous liquid. We show that jets can emerge with very different shapes and velocities, depending on the impact parameters, thus generating droplets with various initial sizes and velocities. After presenting the jet velocity and top drop radius variation as a function of the impact parameters, we discuss the influence of the liquid parameters on the jet velocity. This allows us to define two different regimes: the singular jet and the cavity jet regimes, where the mechanisms leading to the cavity retraction and subsequent jet dynamics are drastically different. In particular, we demonstrate that in the first regime, a singular capillary wave collapse sparks the whole jet dynamics, making the jet's fast, thin, liquid parameters dependent and barely reproducible. On the contrary, in the cavity jet regime, defined for higher impact Froude numbers, the jets are fat and slow. We show that jet velocity is simply proportional to the capillary velocity √{γ /ρlDd }, where γ is the liquid surface tension, ρl the liquid density, and Dd the impacting drop diameter, and it is in particular independent of viscosity, impact velocity, and gravity, even though the cavity is larger than the capillary length. Finally, we demonstrate that capillary wave collapse and cavity retraction are correlated in the singular regime and decorrelated in the cavity jet regime.

Analysis of flow dynamics through small diameter gas sampling systems

NASA Technical Reports Server (NTRS)

Brown, K. G.

1984-01-01

The removal of gas material through a capillary opening in a surface is analyzed. The gas, from which the sample is removed, is moving past the surface at supersonic velocities. A variety of possible conditions of temperature, pressure and composition are discussed in an effort to emulate conditions that might be found at the surface of a vehicle traversing the altitude range 100-50 km, or might exist at the surface of a model in the stream of a high enthalpy wind tunnel. Aspects discussed include: (1) the throughput of the capillary for conditions of different lengths and different L/a (length/radius) ratios; (2) the total throughput when the surface in question contains many hundreds of these capillaries; (3) the effect of the capillaries upon the composition of the analyzed gas; (4) the effect of the capillary or capillaries upon the gas stream itself; and (5) the implications of the calculations upon the possible implementation of this type of device as an inlet for a mass spectrometer to be developed for analyzing the upper atmosphere.

Capillary red blood cell velocimetry by phase-resolved optical coherence tomography.

PubMed

Tang, Jianbo; Erdener, Sefik Evren; Fu, Buyin; Boas, David A

2017-10-01

We present a phase-resolved optical coherence tomography (OCT) method to extend Doppler OCT for the accurate measurement of the red blood cell (RBC) velocity in cerebral capillaries. OCT data were acquired with an M-mode scanning strategy (repeated A-scans) to account for the single-file passage of RBCs in a capillary, which were then high-pass filtered to remove the stationary component of the signal to ensure an accurate measurement of phase shift of flowing RBCs. The angular frequency of the signal from flowing RBCs was then quantified from the dynamic component of the signal and used to calculate the axial speed of flowing RBCs in capillaries. We validated our measurement by RBC passage velocimetry using the signal magnitude of the same OCT time series data.

Characterizing the interaction between enantiomers of eight psychoactive drugs and highly sulfated-β-cyclodextrin by counter-current capillary electrophoresis.

PubMed

Asensi-Bernardi, Lucía; Escuder-Gilabert, Laura; Martín-Biosca, Yolanda; Sagrado, Salvador; Medina-Hernández, María José

2014-01-01

The estimation of apparent binding constants and limit mobilities of the complexes of the enantiomers that characterize the interaction of enantiomers with chiral selectors, in this case highly sulfated β-cyclodextrin, was approached using a simple and economic electrophoretic modality, the complete filling technique (CFT) in counter-current mode. The enantiomers of eight psychoactive drugs, four antihistamines (dimethindene, promethazine, orphenadrine and terfenadine) and four antidepressants (bupropion, fluoxetine, nomifensine and viloxazine) were separated for the first time for this cyclodextrin (CD). Estimations of thermodynamic and electrophoretic enantioselectivies were also performed. Results indicate that, in general, thermodynamic enantioselectivity is the main component explaining the high resolution found, but also one case suggests that electrophoretic enantioselectivity itself is enough to obtain a satisfactory resolution. CFT results advantageous compared with conventional capillary electrophoresis (CE) and partial filling technique (PFT) for the study of the interaction between drugs and chiral selectors. It combines the use of a simple fitting model (as in CE), when the enantiomers do not exit the chiral selector plug during the separation (i.e. mobility of electroosmotic flow larger than mobility of CD), and drastic reduction of the consumption (and cost; ~99.7%) of the CD reagent (as in PFT) compared with the conventional CE. Copyright © 2013 John Wiley & Sons, Ltd.

Thermal performance analysis of a flat heat pipe working with carbon nanotube-water nanofluid for cooling of a high heat flux heater

NASA Astrophysics Data System (ADS)

Arya, A.; Sarafraz, M. M.; Shahmiri, S.; Madani, S. A. H.; Nikkhah, V.; Nakhjavani, S. M.

2018-04-01

Experimental investigation on the thermal performance of a flat heat pipe working with carbon nanotube nanofluid is conducted. It is used for cooling a heater working at high heat flux conditions up to 190 kW/m2. The heat pipe is fabricated from aluminium and is equipped with rectangular fin for efficient cooling of condenser section. Inside the heat pipe, a screen mesh was inserted as a wick structure to facilitate the capillary action of working fluid. Influence of different operating parameters such as heat flux, mass concentration of carbon nanotubes and filling ratio of working fluid on thermal performance of heat pipe and its thermal resistance are investigated. Results showed that with an increase in heat flux, the heat transfer coefficient in evaporator section of the heat pipe increases. For filling ratio, however, there is an optimum value, which was 0.8 for the test heat pipe. In addition, CNT/water enhanced the heat transfer coefficient up to 40% over the deionized water. Carbon nanotubes intensified the thermal performance of wick structure by creating a fouling layer on screen mesh structure, which changes the contact angle of liquid with the surface, intensifying the capillary forces.

A Comprehensive Fluid Dynamic-Diffusion Model of Blood Microcirculation with Focus on Sickle Cell Disease

NASA Astrophysics Data System (ADS)

Le Floch, Francois; Harris, Wesley L.

2009-11-01

A novel methodology has been developed to address sickle cell disease, based on highly descriptive mathematical models for blood flow in the capillaries. Our investigations focus on the coupling between oxygen delivery and red blood cell dynamics, which is crucial to understanding sickle cell crises and is unique to this blood disease. The main part of our work is an extensive study of blood dynamics through simulations of red cells deforming within the capillary vessels, and relies on the use of a large mathematical system of equations describing oxygen transfer, blood plasma dynamics and red cell membrane mechanics. This model is expected to lead to the development of new research strategies for sickle cell disease. Our simulation model could be used not only to assess current researched remedies, but also to spur innovative research initiatives, based on our study of the physical properties coupled in sickle cell disease.

Self organized spatio-temporal structure within the fractured Vadose Zone: The influence of dynamic overloading at fracture intersections

NASA Astrophysics Data System (ADS)

LaViolette, Randall A.; Glass, Robert J.

2004-09-01

Under low flow conditions (where gravity and capillary forces dominate) within an unsaturated fracture network, fracture intersections act as capillary barriers to integrate flow from above and then release it as a pulse below. Water exiting a fracture intersection is often thought to enter the single connected fracture with the lowest invasion pressure. When the accumulated volume varies between intersections, the smaller volume intersections can be overloaded to cause all of the available fractures exiting an intersection to flow. We included the dynamic overloading process at fracture intersections within our previously discussed model where intersections were modeled as tipping buckets connected within a two-dimensional diamond lattice. With dynamic overloading, the flow behavior transitioned smoothly from diverging to converging flow with increasing overload parameter, as a consequence of a heterogeneous field, and they impose a dynamic structure where additional pathways activate or deactivate in time.

Study on a neon cryogenic oscillating heat pipe with long heat transport distance

NASA Astrophysics Data System (ADS)

Liang, Qing; Li, Yi; Wang, Qiuliang

2018-06-01

An experimental study is carried out to study the heat transfer characteristics of a cryogenic oscillating heat pipe (OHP) with long heat transport distance. The OHP is made up of a capillary tube with an inner diameter of 1.0 mm and an outer diameter of 2.0 mm. The working fluid is neon, and the length of the adiabatic section is 480 mm. Tests are performed with the different heat inputs, liquid filling ratios and condenser temperature. For the cryogenic OHP with a liquid filling ratio of 30.7% at the condenser temperature of 28 K, the effective thermal conductivity is 3466-30,854 W/m K, and the maximum transfer power is 35.60 W. With the increment of the heat input, the effective thermal conductivity of the cryogenic OHP increases at the liquid filling ratios of 30.7% and 38.5%, while it first increases and then decreases at the liquid filling ratios of 15.2% and 23.3%. Moreover, the effective thermal conductivity increases with decreasing liquid filling ratio at the small heat input, and the maximum transfer power first increases and then decreases with increasing liquid filling ratio. Finally, it is found that the thermal performance of the cryogenic OHP can be improved by increasing the condenser temperature.

Coherent X-ray Scattering from Liquid-Air Interfaces

NASA Astrophysics Data System (ADS)

Shpyrko, Oleg

Advances in synchrotron x-ray scattering techniques allow studies of structure and dynamics of liquid surfaces with unprecedented resolution. I will review x-ray scattering measurements of thermally excited capillary fluctuations in liquids, thin polymer liquid films and polymer surfaces in confined geometry. X-ray Diffuse scattering profile due to Debye-Waller like roughening of the surface allows to probe the distribution of capillary fluctuations over a wide range of length scales, while using X-ray Photon Correlation Spectroscopy (XPCS) one is able to directly couple to nanoscale dynamics of these surface fluctuations, over a wide range of temporal and spacial scales. I will also discuss recent XPCS measurements of lateral diffusion dynamics in Langmuir monolayers assembled at the liquid-air interface. This research was supported by NSF CAREER Grant 0956131.

Cellular context–mediated Akt dynamics regulates MAP kinase signaling thresholds during angiogenesis

PubMed Central

Hellesøy, Monica; Lorens, James B.

2015-01-01

The formation of new blood vessels by sprouting angiogenesis is tightly regulated by contextual cues that affect angiogeneic growth factor signaling. Both constitutive activation and loss of Akt kinase activity in endothelial cells impair angiogenesis, suggesting that Akt dynamics mediates contextual microenvironmental regulation. We explored the temporal regulation of Akt in endothelial cells during formation of capillary-like networks induced by cell–cell contact with vascular smooth muscle cells (vSMCs) and vSMC-associated VEGF. Expression of constitutively active Akt1 strongly inhibited network formation, whereas hemiphosphorylated Akt1 epi-alleles with reduced kinase activity had an intermediate inhibitory effect. Conversely, inhibition of Akt signaling did not affect endothelial cell migration or morphogenesis in vSMC cocultures that generate capillary-like structures. We found that endothelial Akt activity is transiently blocked by proteasomal degradation in the presence of SMCs during the initial phase of capillary-like structure formation. Suppressed Akt activity corresponded to the increased endothelial MAP kinase signaling that was required for angiogenic endothelial morphogenesis. These results reveal a regulatory principle by which cellular context regulates Akt protein dynamics, which determines MAP kinase signaling thresholds necessary drive a morphogenetic program during angiogenesis. PMID:26023089

Developing a capillary electrophoresis based method for dynamically monitoring enzyme cleavage activity using quantum dots-peptide assembly.

PubMed

Wang, Jianhao; Fan, Jie; Liu, Li; Ding, Shumin; Liu, Xiaoqian; Wang, Jianpeng; Gao, Liqian; Chattopadhaya, Souvik; Miao, Peng; Xia, Jiang; Qiu, Lin; Jiang, Pengju

2017-10-01

Herein, a novel assay has been developed for monitoring PreScission protease (His-PSP) mediated enzyme cleavage of ATTO 590 labeled peptide substrate (ATTO-LEV). This novel method is based on combining the use of capillary electrophoresis and fluorescence detection (CE-FL) to dynamically monitor the enzyme cleavage activity. A multivalent peptide substrate was first constructed by immobilizing His-tagged ATTO 590 labeled peptide substrate (ATTO-LEVH6) onto the surface of CdSe/ZnS quantum dots (QDs). Once successfully immobilized, the novel multivalent peptide substrate resulted in the Förster resonance energy transfer (FRET) from QDs to ATTO 590. The ATTO-LEVH6-QD assembly was then incubated with His-PSP to study the proteolytic cleavage of surface bound ATTO-LEVH6 by CE-FL. Our data suggests that PreScission-mediated proteolytic cleavage is enzyme concentration- and incubation time-dependent. By combining capillary electrophoresis, QDs and FRET, our study herein not only provides a new method for the detection and dynamically monitoring of PSP enzyme cleavage activity, but also can be extended to the detection of many other enzymes and proteases. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A microvascular compartment model validated using 11C-methylglucose liver PET in pigs

NASA Astrophysics Data System (ADS)

Munk, Ole L.; Keiding, Susanne; Baker, Charles; Bass, Ludvik

2018-01-01

The standard compartment model (CM) is widely used to analyse dynamic PET data. The CM is fitted to time-activity curves to estimate rate constants that describe the transport of a tracer between well-mixed compartments. The aim of this study was to develop and validate a more realistic microvascular compartment model (MCM) that includes capillary tracer concentration gradients, backflux from cells into the perfused capillaries and multiple re-uptakes during the passage through a capillary. The MCM incorporates only parameters with clear physiological meaning, it is easy to implement, and it does not require numerical solution. We compared the MCM and CM for the analysis of 3 min dynamic PET data of pig livers (N  =  5) following injection of 11C-methylglucose. During PET scans, the tracer concentrations in blood were measured in the abdominal aorta, portal vein and liver vein by manual sampling. We found that the MCM outperformed the CM and that dynamic PET data include information which cannot be extracted using standard CM. The MCM fitted dynamic PET data better than the CM (Akaike values were 46  ±  4 for best MCM fits, and 82  ±  8 for best CM fits; mean  ±  standard deviation) and extracted physiologically reasonable parameter estimates such as blood perfusion that were in agreement with independent measurements. The difference between model-independent perfusion estimates and the best MCM perfusion estimates was  -0.01  ±  0.05 ml/ml/min, whereas the difference was 0.30  ±  0.13 ml/ml/min using the CM. In addition, the MCM predicted the time course of concentrations in the liver vein, a prediction fundamentally unobtainable using the CM as it does not return tracer backflux from cells to capillary blood. The results demonstrate the benefit of using models that include more physiology and that models including concentration gradients should be preferred when analysing the blood-cell exchange of any tracer in any capillary bed.

Development and demonstration of a water-window soft x-ray microscope using a Z-pinching capillary discharge source

NASA Astrophysics Data System (ADS)

Nawaz, M. F.; Jancarek, Alexandr; Nevrkla, Michal; Duda, Martin Jakub; Pina, Ladislav

2017-05-01

The development and demonstration of a soft X-ray (SXR) microscope, based on a Z-pinching capillary discharge source has been realized. The Z-pinching plasma acts as a source of SXR radiation. A ceramic capacitor bank is pulsed charged up to 80 kV, and discharged through a pre- ionized nitrogen filled ceramic capillary. The discharge current has an amplitude of 25 kA. Working within the water-window spectral region (λ = 2.88 nm), corresponding to the 1s2-1s2p quantum transition of helium-like nitrogen (N5+), the microscope has a potential in exploiting the natural contrast existing between the K-absorption edges of carbon and oxygen as the main constituents of biological materials, and hence imaging them with high spatial resolution. The SXR microscope uses the grazing incidence ellipsoidal condenser mirror for the illumination, and the Fresnel zone plate optics for the imaging of samples onto a BI-CCD camera. The half- pitch spatial resolution of 100 nm [1] was achieved, as demonstrated by the knife-edge test. In order to enhance the photon-flux at the sample plane, a new scheme for focusing the radiation, from multiple capillary sources has been investigated. Details about the source, and the construction of the microscope are presented and discussed.

Polymer microfluidic device replacing fluids using only capillary force

NASA Astrophysics Data System (ADS)

Chung, Kwang Hyo; Lee, Dae Sik; Yang, Haesik; Kim, Sung Jin; Pyo, Hyun Bong

2005-02-01

A novel polymer microfluidic device for self-wash using only capillary force is presented. A liquid filled in a reaction chamber is replaced by another liquid with no external actuation. All the fluidic actuations in the device is pre-programmed about time and sequence, and accomplished by capillary force naturally. Careful design is necessary for exact actions. The fluidic conduits were designed by the newly derived theoretical equations about the capillary stop pressure and flow time. Simulations using CFD-ACE+ were conducted to check the validity of theory and the performance of the chip. These analytic results were consistent with experimental ones. The chip was made of polymers for the purpose of single use and low price. It was fabricated by sealing the hot-embossed PMMA substrate with a PET film. For simpler fabrication, the chip was of a single height. The embossing master was produced from a nickel-electroplating on a SU8-patterned Ni-plate followed by CMP. The contact angles of liquids on substrates were manipulated through the mixing of surfactants, and the temporal variations were monitored for a more exact design. The real actuation steps in experiment revealed the stable performance of selfwash, and coincided well with the designed ones. The presented microfluidic method can be applicable to other LOCs of special purposes through simple modification. For example, array or serial types would be possible for multiple selfwashes.

Confocal laser-induced fluorescence detector for narrow capillary system with yoctomole limit of detection.

PubMed

Weaver, Mitchell T; Lynch, Kyle B; Zhu, Zaifang; Chen, Huang; Lu, Joann J; Pu, Qiaosheng; Liu, Shaorong

2017-04-01

Laser-induced fluorescence (LIF) detectors for low-micrometer and sub-micrometer capillary on-column detection are not commercially available. In this paper, we describe in details how to construct a confocal LIF detector to address this issue. We characterize the detector by determining its limit of detection (LOD), linear dynamic range (LDR) and background signal drift; a very low LOD (~70 fluorescein molecules or 12 yoctomole fluorescein), a wide LDR (greater than 3 orders of magnitude) and a small background signal drift (~1.2-fold of the root mean square noise) are obtained. For detecting analytes inside a low-micrometer and sub-micrometer capillary, proper alignment is essential. We present a simple protocol to align the capillary with the optical system and use the position-lock capability of a translation stage to fix the capillary in position during the experiment. To demonstrate the feasibility of using this detector for narrow capillary systems, we build a 2-μm-i.d. capillary flow injection analysis (FIA) system using the newly developed LIF prototype as a detector and obtain an FIA LOD of 14 zeptomole fluorescein. We also separate a DNA ladder sample by bare narrow capillary - hydrodynamic chromatography and use the LIF prototype to monitor the resolved DNA fragments. We obtain not only well-resolved peaks but also the quantitative information of all DNA fragments. Copyright © 2016 Elsevier B.V. All rights reserved.

Occlusion and rupture of ex vivo capillary bifurcation due to acoustic droplet vaporization

NASA Astrophysics Data System (ADS)

Feng, Yi; Qin, Dui; Zhang, Jun; Zhang, Lei; Bouakaz, Ayache; Wan, Mingxi

2018-06-01

Gas embolotherapy (GE) consists in the occlusion of tumor blood vessels using gas emboli induced by acoustic droplet vaporization (ADV), to create tumor starvation and localized drug delivery. Therefore, the occlusion and rupture of capillary bifurcation due to ADV was investigated in an ex vivo rat mesentery model using a confocal acousto-optical high-speed microscope system. Following ADV bubble formation, coalescence, and translational movement, the growing bubbles lodged in and then occluded two different capillary bifurcations. Capillary rupture was induced at the bubble lodging area, immediately followed by gas extravasation and bubble dislodging. Before and after bubble lodgment/occlusion, a local microvessel invagination was observed due to the interactions between ADV bubbles and the microvessel itself, indicating a contribution to the capillary rupture. Understanding the transient dynamics of ADV bubble, the bubble-microvessel interaction and the consequent mechanical bio-effects in GE is of the paramount importance for developing and applying this approach in clinical practice.

Colloidal Asphaltene Deposition and Aggregation in Capillary Flow: Experiments and Mesoscopic Simulation

NASA Astrophysics Data System (ADS)

Boek, Edo S.; Ladva, Hemant K.; Crawshaw, John P.; Padding, Johan T.

2008-07-01

The aggregation and deposition of colloidal asphaltene in reservoir rock is a significant problem in the oil industry. To obtain a fundamental understanding of this phenomenon, we have studied the deposition and aggregation of colloidal asphaltene in capillary flow by experiment and simulation. For the simulation, we have used the stochastic rotation dynamics (SRD) method, in which the solvent hydrodynamic emerges from the collisions between the solvent particles, while the Brownian motion emerges naturally from the interactions between the colloidal asphaltene particles and the solvent. The asphaltene colloids interact through a screened Coulomb potential. We vary the well depth ɛ∝ and the flow rate v to obtain Peflow≫1 (hydrodynamic interactions dominate) and Re≪1 (Stokes flow). In the simulations, we impose a pressure drop over the capillary length and measure the corresponding solvent flow rate. We observe that the transient solvent flow rate decreases when the asphaltene particles become more "sticky". For a well depth ɛ∝ = 2kBT, a monolayer deposits on the capillary wall. With an increasing well depth, the capillary becomes totally blocked. The clogging is transient for ɛ∝ = 5kBT, but appears to be permanent for ɛ∝ = 10-20 kBT. We compare our simulation results with flow experiments in glass capillaries, where we use extracted asphaltenes in toluene, reprecipitated with n-heptane. In the experiments, the dynamics of asphaltene precipitation and deposition were monitored in a slot capillary using optical microscopy under flow conditions similar to those used in the simulation. Maintaining a constant flow rate of 5 μL min-1, we found that the pressure drop across the capillary first increased slowly, followed by a sharp increase, corresponding to a complete local blockage of the capillary. Doubling the flow rate to 10 μL min-1, we observe that the initial deposition occurs faster but the deposits are subsequently entrained by the flow. We calculate the change in the dimensionless permeability as a function of time for both experiment and simulation. By matching the experimental and simulation results, we obtain information about (1) the interaction potential well depth for the particular asphaltenes used in the experiments and (2) the flow conditions associated with the asphaltene deposition process.

Infrared thermography of evaporative fluxes and dynamics of salt deposition on heterogeneous porous surfaces

NASA Astrophysics Data System (ADS)

Nachshon, Uri; Shahraeeni, Ebrahim; Or, Dani; Dragila, Maria; Weisbrod, Noam

2011-12-01

Evaporation of saline solutions from porous media, common in arid areas, involves complex interactions between mass transport, energy exchange and phase transitions. We quantified evaporation of saline solutions from heterogeneous sand columns under constant hydraulic boundary conditions to focus on effects of salt precipitation on evaporation dynamics. Mass loss measurements and infrared thermography were used to quantify evaporation rates. The latter method enables quantification of spatial and temporal variability of salt precipitation to identify its dynamic effects on evaporation. Evaporation from columns filled with texturally-contrasting sand using different salt solutions revealed preferential salt precipitation within the fine textured domains. Salt precipitation reduced evaporation rates from the fine textured regions by nearly an order of magnitude. In contrast, low evaporation rates from coarse-textured regions (due to low capillary drive) exhibited less salt precipitation and consequently less evaporation rate suppression. Experiments provided insights into two new phenomena: (1) a distinct increase in evaporation rate at the onset of evaporation; and (2) a vapor pumping mechanism related to the presence of a salt crust over semidry media. Both phenomena are related to local vapor pressure gradients established between pore water and the surface salt crust. Comparison of two salts: NaCl and NaI, which tend to precipitate above the matrix surface and within matrix pores, respectively, shows a much stronger influence of NaCl on evaporation rate suppression. This disparity reflects the limited effect of NaI precipitation on matrix resistivity for solution and vapor flows.

A microliter capillary rheometer for characterization of protein solutions.

PubMed

Hudson, Steven D; Sarangapani, Prasad; Pathak, Jai A; Migler, Kalman B

2015-02-01

Rheometry is an important characterization tool for therapeutic protein solutions because it determines syringeability and relates indirectly to solution stability and thermodynamic interactions. Despite the maturity of rheometry, there remains a need for a rheometer that meets the following three needs of the biopharamaceutical industry: small volume; large dynamic range of shear rates; and no air-sample interface. Here, we report the development of a miniaturized capillary rheometer that meets these needs and is potentially scalable to a multiwell format. These measurements consume only a few microliters of sample and have an uncertainty of a few percent. We demonstrate its performance on monoclonal antibody solutions at different concentrations and temperatures. The instrument has a dynamic range of approximately three decades (in shear rate) and can measure Newtonian, shear thinning, and yielding behaviors, which are representative of the different solution behaviors typically encountered. We compare our microliter capillary rheometer with existing instruments to describe the range of parameter space covered by our device. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

Infiltration of MHD liquid into a deformable porous material

NASA Astrophysics Data System (ADS)

Naseem, Anum; Mahmood, Asif; Siddique, J. I.; Zhao, Lifeng

2018-03-01

We analyze the capillary rise dynamics for magnetohydrodynamics (MHD) fluid flow through deformable porous material in the presence of gravity effects. The modeling is performed using mixture theory approach and mathematical manipulation yields a nonlinear free boundary problem. Due to the capillary rise action, the pressure gradient in the liquid generates a stress gradient that results in the deformation of porous substrate. The capillary rise process for MHD fluid slows down as compared to Newtonian fluid case. Numerical solutions are obtained using a method of lines approach. The graphical results are presented for important physical parameters, and comparison is presented with Newtonian fluid case.

Stability analysis for capillary channel flow: 1d and 3d computations

NASA Astrophysics Data System (ADS)

Grah, Aleksander; Klatte, Jörg; Dreyer, Michael E.

The subject of the presentation are numerical studies on capillary channel flow, based on results of the sounding rocket TEXUS experiments. The flow through a capillary channel is established by a gear pump at the outlet. The channel, consists of two parallel glass plates with a width of 25 mm, a gap of 10 mm and a length of 12 mm. The meniscus of a compensation tube maintains a constant system pressure. Steady and dynamic pressure effects in the system force the surfaces to bend inwards. A maximum flow rate is achieved when the free surface collapses and gas ingestion occurs at the outlet. This critical flow rate depends on the channel geometry, the flow regime and the liquid properties. The aim of the experiments is the determination of the free surface shape and to find the maximum flow rate. In order to study the unsteady liquid loop behavior, a dimensionless one-dimensional model and a corresponding three-dimensional model were developed. The one-dimensional model is based on the unsteady Bernoulli equation, the unsteady continuity equation and geometrical conditions for the surface curvature and the flow cross-section. The experimental and evaluated contour data show good agreement for a sequence of transient flow rate perturbations. In the case of steady flow at maximum flow rate, when the "choking" effect occurs, the surfaces collapse and cause gas ingestion into the channel. This effect is related to the Speed Index. At the critical flow rate the Speed Index reaches the value 1, in analogy to the Mach Number. Unsteady choking does not necessarily cause surface collapse. We show, that temporarily Speed Index values exceeding One may be achieved for a perfectly stable supercritical dynamic flow. As a supercritical criterion for the dynamic free surface stability we define a Dynamic Index considering the local capillary pressure and the convective pressure, which is a function of the local velocity. The Dynamic Index is below One for stable flow while D = 1 indicates surface collapse. This studies lead to a stability diagram, which defines the limits of flow dynamics and the maximum unsteady flow rate.

Breakup Behavior of a Capillary Bridge on a Hydrophobic Stripe Separating Two Hydrophilic Stripes

NASA Astrophysics Data System (ADS)

Hartmann, Maximilian; Hardt, Steffen

2017-11-01

The breakup dynamics of a capillary bridge on a hydrophobic area between two liquid filaments occupying two parallel hydrophilic stripes is studied experimentally. In addition calculations with the finite-element software Surface Evolver are performed to obtain the corresponding stable minimal surfaces. Droplets of de-ionized water are placed on substrates with alternating hydrophilic and hydrophobic stripes of different width. Their volume decreases by evaporation. This results in a droplet shaped as the letter ``H'' covering two hydrophilic stripes separated by one hydrophobic stripe. The width of the capillary bridge d(t) on the hydrophobic stripe during the breakup process is observed using a high-speed camera mounted on a bright-field microscope. The results of the experiments and the numerical studies show that the critical width dcrit, indicating the point where the capillary bridge becomes unstable, mainly depends on the width ratio of the hydrophilic and hydrophobic stripes. It is found that the time derivative of d(t) first decreases after dcrit has been reached. The final breakup dynamics then follows a t 2 / 3 scaling. We kindly acknowledge the financial support by the German Research Foundation (DFG) within the Collaborative Research Centre 1194 ``Interaction of Transport and Wetting Processes'', Project A02a.

Controlling retention, selectivity and magnitude of EOF by segmented monolithic columns consisting of octadecyl and naphthyl monolithic segments--applications to RP-CEC of both neutral and charged solutes.

PubMed

Karenga, Samuel; El Rassi, Ziad

2011-04-01

Monolithic capillaries made of two adjoining segments each filled with a different monolith were introduced for the control and manipulation of the electroosmotic flow (EOF), retention and selectivity in reversed phase-capillary electrochromatography (RP-CEC). These columns were called segmented monolithic columns (SMCs) where one segment was filled with a naphthyl methacrylate monolith (NMM) to provide hydrophobic and π-interactions, while the other segment was filled with an octadecyl acrylate monolith (ODM) to provide solely hydrophobic interaction. The ODM segment not only provided hydrophobic interactions but also functioned as the EOF accelerator segment. The average EOF of the SMC increased linearly with increasing the fractional length of the ODM segment. The neutral SMC provided a convenient way for tuning EOF, selectivity and retention in the absence of annoying electrostatic interactions and irreversible solute adsorption. The SMCs allowed the separation of a wide range of neutral solutes including polycyclic aromatic hydrocarbons (PAHs) that are difficult to separate using conventional alkyl-bonded stationary phases. In all cases, the k' of a given solute was a linear function of the fractional length of the ODM or NMM segment in the SMCs, thus facilitating the tailoring of a given SMC to solve a given separation problem. At some ODM fractional length, the fabricated SMC allowed the separation of charged solutes such as peptides and proteins that could not otherwise be achieved on a monolithic column made from NMM as an isotropic stationary phase due to the lower EOF exhibited by this monolith. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Living cell manipulation, manageable sampling, and shotgun picoliter electrospray mass spectrometry for profiling metabolites.

PubMed

Gholipour, Yousef; Erra-Balsells, Rosa; Hiraoka, Kenzo; Nonami, Hiroshi

2013-02-01

A modified cell pressure probe and an online Orbitrap mass spectrometer were used to sample in situ plant single cells without any additional manipulation. The cell pressure probe, a quartz capillary tip filled with an oil mixture, was penetrated to various depths into parenchyma cells of tulip bulb scale, followed by a hydraulic continuity test to determine the exact location of the tip inside target cells. The operation was conducted under a digital microscope, and the capillary tip was photographed to calculate the volume of the cell sap sucked. The cell sap sample was then directly nebulized/ionized under high-voltage conditions at the entrance of the mass spectrometer. Several sugars, amino acids, organic acids, vitamins, fatty acids, and secondary metabolites were detected. Because picoliter solutions can be accurately handled and measured, known volumes of standard solutions can be added to cell sap samples inside the capillary tip to be used as references for metabolite characterization and relative quantitation. The high precision and sensitivity of the cell pressure probe and Orbitrap mass spectrometer allow for the manipulation and analysis of both femtoliter cell sap samples and standard solutions. Copyright © 2012 Elsevier Inc. All rights reserved.

Nanokit for single-cell electrochemical analyses.

PubMed

Pan, Rongrong; Xu, Mingchen; Jiang, Dechen; Burgess, Jame D; Chen, Hong-Yuan

2016-10-11

The development of more intricate devices for the analysis of small molecules and protein activity in single cells would advance our knowledge of cellular heterogeneity and signaling cascades. Therefore, in this study, a nanokit was produced by filling a nanometer-sized capillary with a ring electrode at the tip with components from traditional kits, which could be egressed outside the capillary by electrochemical pumping. At the tip, femtoliter amounts of the kit components were reacted with the analyte to generate hydrogen peroxide for the electrochemical measurement by the ring electrode. Taking advantage of the nanotip and small volume injection, the nanokit was easily inserted into a single cell to determine the intracellular glucose levels and sphingomyelinase (SMase) activity, which had rarely been achieved. High cellular heterogeneities of these two molecules were observed, showing the significance of the nanokit. Compared with the current methods that use a complicated structural design or surface functionalization for the recognition of the analytes, the nanokit has adapted features of the well-established kits and integrated the kit components and detector in one nanometer-sized capillary, which provides a specific device to characterize the reactivity and concentrations of cellular compounds in single cells.

Biodegradation of phenol, salicylic acid, benzenesulfonic acid, and iomeprol by Pseudomonas fluorescens in the capillary fringe

NASA Astrophysics Data System (ADS)

Hack, Norman; Reinwand, Christian; Abbt-Braun, Gudrun; Horn, Harald; Frimmel, Fritz H.

2015-12-01

Mass transfer and biological transformation phenomena in the capillary fringe were studied using phenol, salicylic acid, benzenesulfonic acid, and the iodinated X-ray contrast agent iomeprol as model organic compounds and the microorganism strain Pseudomonas fluorescens. Three experimental approaches were used: Batch experiments (uniform water saturation and transport by diffusion), in static columns (with a gradient of water saturation and advective transport in the capillaries) and in a flow-through cell (with a gradient of water saturation and transport by horizontal and vertical flow: 2-dimension flow-through microcosm). The reactors employed for the experiments were filled with quartz sand of defined particle size distribution (dp = 200…600 μm, porosity ε = 0.42). Batch experiments showed that phenol and salicylic acid have a high, whereas benzenesulfonic acid and iomeprol have a quite low potential for biodegradation under aerobic conditions and in a matrix nearly close to water saturation. Batch experiments under anoxic conditions with nitrate as electron acceptor revealed that the biodegradation of the model compounds was lower than under aerobic conditions. Nevertheless, the experiments showed that the moisture content was also responsible for an optimized transport in the liquid phase of a porous medium. Biodegradation in the capillary fringe was found to be influenced by both the moisture content and availability of the dissolved substrate, as seen in static column experiments. The gas-liquid mass transfer of oxygen also played an important role for the biological activity. In static column experiments under aerobic conditions, the highest biodegradation was found in the capillary fringe (e.g. βt/β0 (phenol) = 0 after t = 6 d) relative to the zone below the water table and unsaturated zone. The highest biodegradation occurred in the flow-through cell experiment where the height of the capillary fringe was largest.

Biodegradation of phenol, salicylic acid, benzenesulfonic acid, and iomeprol by Pseudomonas fluorescens in the capillary fringe.

PubMed

Hack, Norman; Reinwand, Christian; Abbt-Braun, Gudrun; Horn, Harald; Frimmel, Fritz H

2015-12-01

Mass transfer and biological transformation phenomena in the capillary fringe were studied using phenol, salicylic acid, benzenesulfonic acid, and the iodinated X-ray contrast agent iomeprol as model organic compounds and the microorganism strain Pseudomonas fluorescens. Three experimental approaches were used: Batch experiments (uniform water saturation and transport by diffusion), in static columns (with a gradient of water saturation and advective transport in the capillaries) and in a flow-through cell (with a gradient of water saturation and transport by horizontal and vertical flow: 2-dimension flow-through microcosm). The reactors employed for the experiments were filled with quartz sand of defined particle size distribution (dp=200...600 μm, porosity ε=0.42). Batch experiments showed that phenol and salicylic acid have a high, whereas benzenesulfonic acid and iomeprol have a quite low potential for biodegradation under aerobic conditions and in a matrix nearly close to water saturation. Batch experiments under anoxic conditions with nitrate as electron acceptor revealed that the biodegradation of the model compounds was lower than under aerobic conditions. Nevertheless, the experiments showed that the moisture content was also responsible for an optimized transport in the liquid phase of a porous medium. Biodegradation in the capillary fringe was found to be influenced by both the moisture content and availability of the dissolved substrate, as seen in static column experiments. The gas-liquid mass transfer of oxygen also played an important role for the biological activity. In static column experiments under aerobic conditions, the highest biodegradation was found in the capillary fringe (e.g. βt/β0 (phenol)=0 after t=6 d) relative to the zone below the water table and unsaturated zone. The highest biodegradation occurred in the flow-through cell experiment where the height of the capillary fringe was largest. Copyright © 2015 Elsevier B.V. All rights reserved.

Synthesis of capillary pressure curves from post-stack seismic data with the use of intelligent estimators: A case study from the Iranian part of the South Pars gas field, Persian Gulf Basin

NASA Astrophysics Data System (ADS)

Golsanami, Naser; Kadkhodaie-Ilkhchi, Ali; Erfani, Amir

2015-01-01

Capillary pressure curves are important data for reservoir rock typing, analyzing pore throat distribution, determining height above free water level, and reservoir simulation. Laboratory experiments provide accurate data, however they are expensive, time-consuming and discontinuous through the reservoir intervals. The current study focuses on synthesizing artificial capillary pressure (Pc) curves from seismic attributes with the use of artificial intelligent systems including Artificial Neural Networks (ANNs), Fuzzy logic (FL) and Adaptive Neuro-Fuzzy Inference Systems (ANFISs). The synthetic capillary pressure curves were achieved by estimating pressure values at six mercury saturation points. These points correspond to mercury filled pore volumes of core samples (Hg-saturation) at 5%, 20%, 35%, 65%, 80%, and 90% saturations. To predict the synthetic Pc curve at each saturation point, various FL, ANFIS and ANN models were constructed. The varying neural network models differ in their training algorithm. Based on the performance function, the most accurately functioning models were selected as the final solvers to do the prediction process at each of the above-mentioned mercury saturation points. The constructed models were then tested at six depth points of the studied well which were already unforeseen by the models. The results show that the Fuzzy logic and neuro-fuzzy models were not capable of making reliable estimations, while the predictions from the ANN models were satisfyingly trustworthy. The obtained results showed a good agreement between the laboratory derived and synthetic capillary pressure curves. Finally, a 3D seismic cube was captured for which the required attributes were extracted and the capillary pressure cube was estimated by using the developed models. In the next step, the synthesized Pc cube was compared with the seismic cube and an acceptable correspondence was observed.

Investigation of capillary nanosecond discharges in air at moderate pressure: comparison of experiments and 2D numerical modelling

NASA Astrophysics Data System (ADS)

Klochko, Andrei V.; Starikovskaia, Svetlana M.; Xiong, Zhongmin; Kushner, Mark J.

2014-09-01

Nanosecond electrical discharges in the form of ionization waves are of interest for rapidly ionizing and exciting complex gas mixtures to initiate chemical reactions. Operating with a small discharge tube diameter can significantly increase the specific energy deposition and so enable optimization of the initiation process. Analysis of the uniformity of energy release in small diameter capillary tubes will aid in this optimization. In this paper, results for the experimentally derived characteristics of nanosecond capillary discharges in air at moderate pressure are presented and compared with results from a two-dimensional model. The quartz capillary tube, having inner and outer diameters of 1.5 and 3.4 mm, is about 80 mm long and filled with synthetic dry air at 27 mbar. The capillary tube with two electrodes at the ends is inserted into a break of the central wire of a long coaxial cable. A metal screen around the tube is connected to the cable ground shield. The discharge is driven by a 19 kV 35 ns voltage pulse applied to the powered electrode. The experimental measurements are conducted primarily by using a calibrated capacitive probe and back current shunts. The numerical modelling focuses on the fast ionization wave (FIW) and the plasma properties in the immediate afterglow after the conductive plasma channel has been established between the two electrodes. The FIW produces a highly focused region of electric field on the tube axis that sustains the ionization wave that eventually bridges the electrode gap. Results from the model predict FIW propagation speed and current rise time that agree with the experiment.

Resonant Acoustic Measurement of Vapor Phase Transport Phenomenon

NASA Astrophysics Data System (ADS)

Schuhmann, R. J.; Garrett, S. L.; Matson, J. V.

2002-12-01

A major impediment to accurate non steady-state diffusion measurements is the ability to accurately measure and track a rapidly changing gas concentration without disturbing the system. Non-destructive methods that do not interfere with system dynamics have been developed in the past. These methods, however, have tended to be cumbersome or inaccurate at low concentrations. A new experimental approach has been developed to measure gaseous diffusion in free air and through porous materials. The method combines the traditional non steady-state laboratory methodology with resonant acoustic gas analysis. A phase-locked-loop (PLL) resonance frequency tracker is combined with a thermally insulated copper resonator. A piston sealed with a metal bellows excites the fundamental standing wave resonance of the resonator. The PLL maintains a constant phase difference (typically 90§) between the accelerometer mounted on the piston and a microphone near the piston to track the resonance frequency in real time. A capillary or glass bead filled core is fitted into an o-ring sealed opening at the end of the resonator opposite the bellows. The rate at which the tracer gas is replaced by air within the resonator is controlled by the diffusion coefficient of the gas in free air through the capillary (DA) or by the effective diffusion coefficient of the gas through the core (De). The mean molecular weight of the gas mixture in the resonator is directly determined six times each minute from the ratio of the absolute temperature to the square of the fundamental acoustic resonance frequency. Average system stability (temperature divided by frequency squared) is better than 350 ppm. DA values for a 0.3-inch diameter capillary were in excellent agreement with published values. De values for porous media samples (0.5 mm glass beads) of four different lengths (1 through 4 inches) using three different tracer gases (He, CH4, Kr) will be reported. Comments will be offered regarding tracer gas selection and device orientation and their effect on experimental results. [Work supported by the Office of Naval Research.

Using evaporation to control capillary instabilities in micro-systems.

PubMed

Ledesma-Aguilar, Rodrigo; Laghezza, Gianluca; Yeomans, Julia M; Vella, Dominic

2017-12-06

The instabilities of fluid interfaces represent both a limitation and an opportunity for the fabrication of small-scale devices. Just as non-uniform capillary pressures can destroy micro-electrical mechanical systems (MEMS), so they can guide the assembly of novel solid and fluid structures. In many such applications the interface appears during an evaporation process and is therefore only present temporarily. It is commonly assumed that this evaporation simply guides the interface through a sequence of equilibrium configurations, and that the rate of evaporation only sets the timescale of this sequence. Here, we use Lattice-Boltzmann simulations and a theoretical analysis to show that, in fact, the rate of evaporation can be a factor in determining the onset and form of dynamical capillary instabilities. Our results shed light on the role of evaporation in previous experiments, and open the possibility of exploiting diffusive mass transfer to directly control capillary flows in MEMS applications.

Capillary device refilling. [liquid rocket propellant tank tests

NASA Technical Reports Server (NTRS)

Blatt, M. H.; Merino, F.; Symons, E. P.

1980-01-01

An analytical and experimental study was conducted dealing with refilling start baskets (capillary devices) with settled fluid. A computer program was written to include dynamic pressure, screen wicking, multiple-screen barriers, standpipe screens, variable vehicle mass for computing vehicle acceleration, and calculation of tank outflow rate and vapor pullthrough height. An experimental apparatus was fabricated and tested to provide data for correlation with the analytical model; the test program was conducted in normal gravity using a scale-model capillary device and ethanol as the test fluid. The test data correlated with the analytical model; the model is a versatile and apparently accurate tool for predicting start basket refilling under actual mission conditions.

Simulation of the dynamical transmission of several-hundred-keV protons through a conical capillary

NASA Astrophysics Data System (ADS)

Yang, A. X.; Zhu, B. H.; Niu, S. T.; Pan, P.; Han, C. Z.; Song, H. Y.; Shao, J. X.; Chen, X. M.

2018-05-01

The time evolution of the trajectories, angular distributions, and two-dimensional images of intermediate-energy protons being transmitted through a conical capillary was simulated. The simulation results indicate that the charge deposited in the capillary significantly enhances the probability of surface specular scattering and thus greatly enhances the transmission rate. Furthermore, this deposited-charge-assisted specular reflection causes the transmission rate to exhibit an energy dependence proportional to E-1, which is very consistent with the experimental data. After transmission at nonzero tilt angles, the angular distribution of several-hundred-keV protons is far from symmetric, unlike in the case of keV protons.

Micro-engineered remote palpation device for assessing tissue compliance.

PubMed

Hien, M; Yang, T H J; Leung, S K W; Reuben, R L; Habib, F K; McNeill, S A; Schneider, A; McBride, G; Stevens, R; Else, R W

2008-01-01

This paper concerns the operation of the actuator for a prototype micro-engineered mechanical palpation device for deployment via a cystoscope to measure the dynamic mechanical properties of the prostate gland in vivo. The subassembly consists of a 400x200 microm silicon (Si) piston manufactured using deep reactive ion etching (DRIE) housed within an anodically bonded glass-Si-glass sandwiched housing. The micro-channel on the Si layer was formed by powder blasting and contains the micro-piston with one end pointing to the side of the housing and the other facing a via hole leading to a capillary tube. The opening on the side of the housing was sealed by a 5 microm thick silicone membrane which acts to retain the micro-piston and act as a return spring. A 320 microm diameter capillary forms the connection between the micro-channel and a micro-syringe which is operated by a programmable syringe pump to produce a reciprocating action. A pressure sensor is connected along the capillary tube to measure the dynamic pressure within the system. The micro-piston has already been used, separately actuated to measure the dynamic mechanical properties of known viscoelastic materials and prostate tissue. The purpose of the present work is to assess the functionality of the actuator assembly.

A compartment model of alveolar-capillary oxygen diffusion with ventilation-perfusion gradient and dynamics of air transport through the respiratory tract.

PubMed

Jaworski, Jacek; Redlarski, Grzegorz

2014-08-01

This paper presents a model of alveolar-capillary oxygen diffusion with dynamics of air transport through the respiratory tract. For this purpose electrical model representing the respiratory tract mechanics and differential equations representing oxygen membrane diffusion are combined. Relevant thermodynamic relations describing the mass of oxygen transported into the human body are proposed as the connection between these models, as well as the influence of ventilation-perfusion mismatch on the oxygen diffusion. The model is verified based on simulation results of varying exercise intensities and statistical calculations of the results obtained during various clinical trials. The benefit of the approach proposed is its application in simulation-based research aimed to generate quantitative data of normal and pathological conditions. Based on the model presented, taking into account many essential physiological processes and air transport dynamics, comprehensive and combined studies of the respiratory efficiency can be performed. The impact of physical exercise, precise changes in respiratory tract mechanics and alterations in breathing pattern can be analyzed together with the impact of various changes in alveolar-capillary oxygen diffusion. This may be useful in simulation of effects of many severe medical conditions and increased activity level. Copyright © 2014 Elsevier Ltd. All rights reserved.

Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip.

PubMed

Liu, Vincent; Song, Yong-Ak; Han, Jongyoon

2010-06-07

In this paper, we report a novel method for fabricating ion-selective membranes in poly(dimethylsiloxane) (PDMS)/glass-based microfluidic preconcentrators. Based on the concept of capillary valves, this fabrication method involves filling a lithographically patterned junction between two microchannels with an ion-selective material such as Nafion resin; subsequent curing results in a high aspect-ratio membrane for use in electrokinetic sample preconcentration. To demonstrate the concentration performance of this high-aspect-ratio, ion-selective membrane, we integrated the preconcentrator with a surface-based immunoassay for R-Phycoerythrin (RPE). Using a 1x PBS buffer system, the preconcentrator-enhanced immunoassay showed an approximately 100x improvement in sensitivity within 30 min. This is the first time that an electrokinetic microfluidic preconcentrator based on ion concentration polarization (ICP) has been used in high ionic strength buffer solutions to enhance the sensitivity of a surface-based immunoassay.

Magnetic properties of permalloy wires in vycor capillaries

NASA Astrophysics Data System (ADS)

Lubitz, P.; Ayers, J. D.; Davis, A.

1991-11-01

Thin wires of NiFe alloys with compositions near 80% Ni were prepared by melting the alloy in vycor tubes and drawing fibers from the softened glass. The resulting fibers consist of relatively thick-walled vycor capillaries containing permalloy wires filling a few percent of the volume. The wires are continuous over considerable lengths, uniform in circular cross section, nearly free of contact with the walls and can be drawn to have diameters less than 1 μm. Their magnetic properties are generally similar to bulk permalloy, but show a variety of magnetic switching behaviors for fields along the wire axis, depending on composition, wire diameter, and thermal history. As pulled, the wires can show sharp switching, reversible rotation or mixed behavior. This method can produce NiFe alloy wires suitable for use in applications as sensor, memory or inductive elements; other alloys, such as supermalloy and sendust, also can be fabricated as fine wires by this method.

Properties of Cement Mortar Produced from Mixed Waste Materials with Pozzolanic Characteristics.

PubMed

Yen, Chi-Liang; Tseng, Dyi-Hwa; Wu, Yue-Ze

2012-07-01

Waste materials with pozzolanic characteristics, such as sewage sludge ash (SSA), coal combustion fly ash (FA), and granulated blast furnace slag (GBS), were reused as partial cement replacements for making cement mortar in this study. Experimental results revealed that with dual replacement of cement by SSA and GBS and triple replacement by SSA, FA, and GBS at 50% of total cement replacement, the compressive strength (Sc) of the blended cement mortars at 56 days was 93.7% and 92.9% of the control cement mortar, respectively. GBS had the highest strength activity index value and could produce large amounts of CaO to enhance the pozzolanic activity of SSA/FA and form calcium silicate hydrate gels to fill the capillary pores of the cement mortar. Consequently, the Sc development of cement mortar with GBS replacement was better than that without GBS, and the total pore volume of blended cement mortars with GBS/SSA replacement was less than that with FA/SSA replacement. In the cement mortar with modified SSA and GBS at 70% of total cement replacement, the Sc at 56 days was 92.4% of the control mortar. Modifying the content of calcium in SSA also increased its pozzolanic reaction. CaCl(2) accelerated the pozzolanic activity of SSA better than lime did. Moreover, blending cement mortars with GBS/SSA replacement could generate more monosulfoaluminate to fill capillary pores.

Properties of Cement Mortar Produced from Mixed Waste Materials with Pozzolanic Characteristics

PubMed Central

Yen, Chi-Liang; Tseng, Dyi-Hwa; Wu, Yue-Ze

2012-01-01

Abstract Waste materials with pozzolanic characteristics, such as sewage sludge ash (SSA), coal combustion fly ash (FA), and granulated blast furnace slag (GBS), were reused as partial cement replacements for making cement mortar in this study. Experimental results revealed that with dual replacement of cement by SSA and GBS and triple replacement by SSA, FA, and GBS at 50% of total cement replacement, the compressive strength (Sc) of the blended cement mortars at 56 days was 93.7% and 92.9% of the control cement mortar, respectively. GBS had the highest strength activity index value and could produce large amounts of CaO to enhance the pozzolanic activity of SSA/FA and form calcium silicate hydrate gels to fill the capillary pores of the cement mortar. Consequently, the Sc development of cement mortar with GBS replacement was better than that without GBS, and the total pore volume of blended cement mortars with GBS/SSA replacement was less than that with FA/SSA replacement. In the cement mortar with modified SSA and GBS at 70% of total cement replacement, the Sc at 56 days was 92.4% of the control mortar. Modifying the content of calcium in SSA also increased its pozzolanic reaction. CaCl2 accelerated the pozzolanic activity of SSA better than lime did. Moreover, blending cement mortars with GBS/SSA replacement could generate more monosulfoaluminate to fill capillary pores. PMID:22783062

Gas Flow in the Capillary of the Atmosphere-to-Vacuum Interface of Mass Spectrometers

NASA Astrophysics Data System (ADS)

Skoblin, Michael; Chudinov, Alexey; Soulimenkov, Ilia; Brusov, Vladimir; Kozlovskiy, Viacheslav

2017-10-01

Numerical simulations of a gas flow through a capillary being a part of mass spectrometer atmospheric interface were performed using a detailed laminar flow model. The simulated interface consisted of atmospheric and forevacuum volumes connected via a thin capillary. The pressure in the forevacuum volume where the gas was expanding after passing through the capillary was varied in the wide range from 10 to 900 mbar in order to study the volume flow rate as well as the other flow parameters as functions of the pressure drop between the atmospheric and forevacuum volumes. The capillary wall temperature was varied in the range from 24 to 150 °C. Numerical integration of the complete system of Navier-Stokes equations for a viscous compressible gas taking into account the heat transfer was performed using the standard gas dynamic simulation software package ANSYS CFX. The simulation results were compared with experimental measurements of gas flow parameters both performed using our experimental setup and taken from the literature. The simulated volume flow rates through the capillary differed no more than by 10% from the measured ones over the entire pressure and temperatures ranges. A conclusion was drawn that the detailed digital laminar model is able to quantitatively describe the measured gas flow rates through the capillaries under conditions considered. [Figure not available: see fulltext.

Gas Flow in the Capillary of the Atmosphere-to-Vacuum Interface of Mass Spectrometers.

PubMed

Skoblin, Michael; Chudinov, Alexey; Soulimenkov, Ilia; Brusov, Vladimir; Kozlovskiy, Viacheslav

2017-10-01

Numerical simulations of a gas flow through a capillary being a part of mass spectrometer atmospheric interface were performed using a detailed laminar flow model. The simulated interface consisted of atmospheric and forevacuum volumes connected via a thin capillary. The pressure in the forevacuum volume where the gas was expanding after passing through the capillary was varied in the wide range from 10 to 900 mbar in order to study the volume flow rate as well as the other flow parameters as functions of the pressure drop between the atmospheric and forevacuum volumes. The capillary wall temperature was varied in the range from 24 to 150 °C. Numerical integration of the complete system of Navier-Stokes equations for a viscous compressible gas taking into account the heat transfer was performed using the standard gas dynamic simulation software package ANSYS CFX. The simulation results were compared with experimental measurements of gas flow parameters both performed using our experimental setup and taken from the literature. The simulated volume flow rates through the capillary differed no more than by 10% from the measured ones over the entire pressure and temperatures ranges. A conclusion was drawn that the detailed digital laminar model is able to quantitatively describe the measured gas flow rates through the capillaries under conditions considered. Graphical Abstract ᅟ.

Influence of Air Discontinuity and Wall Effects on the Measurements of Hydraulic Parameters Under Dynamic Conditions

NASA Astrophysics Data System (ADS)

Looms, M. C.; Jensen, K. H.; Wildenschild, D.; Christensen, B. S.; Gudbjerg, J.

2003-12-01

Both dynamic (one-step) and semi-static (syringe pump) outflow experiments were carried out in the lab to test whether the resulting retention characteristics differed according to experiment type. Three sands of varying uniformity and coarseness were packed in a cylindrical sample holder. Compressed air was used to control the air phase pressure, while water was allowed to drain at atmospheric pressure from the outlet at the bottom of the sample. During the outflow experiments the capillary pressure was measured within the sample holder using a tensiometer connected to a pressure transducer. A medical CT-scanner was used to visualize and quantify the outflow patterns within the sand matrix during selected outflow experiments. Positive vertical shifts in capillary pressure during dynamic experiments were found in all three sand types at saturations close to porosity. The size and shape of the shifts corresponded with the dynamic effects found in previous work on the topic. Furthermore, the shifts were slightly greater in the coarsest and most uniform sand type. Numerical simulations of the one-step experiments using HYDRUS1D and T2VOC showed, however, that one of the basic assumptions when calculating the capillary pressure was most likely violated. The air phase could not be considered to be continuous at all times, and assuming this to be the case would result in positive shifts of the retention curves when running T2VOC. The results of using the CT-scanner showed the importance of achieving a homogeneous packing, since the investigated sand packing turned out to have an area at the edge of the sample holder with a higher porosity. This caused the edge to control the initial drainage. Therefore, the data collected at high saturations could not be expected to adequately describe the hydraulic properties of the inner sand. We also found that the time at which the inner sand commenced drainage coincided with a jump in capillary pressure for the resulting measured retention curve.

Wettability impact on supercritical CO2 capillary trapping: Pore-scale visualization and quantification

NASA Astrophysics Data System (ADS)

Hu, Ran; Wan, Jiamin; Kim, Yongman; Tokunaga, Tetsu K.

2017-08-01

How the wettability of pore surfaces affects supercritical (sc) CO2 capillary trapping in geologic carbon sequestration (GCS) is not well understood, and available evidence appears inconsistent. Using a high-pressure micromodel-microscopy system with image analysis, we studied the impact of wettability on scCO2 capillary trapping during short-term brine flooding (80 s, 8-667 pore volumes). Experiments on brine displacing scCO2 were conducted at 8.5 MPa and 45°C in water-wet (static contact angle θ = 20° ± 8°) and intermediate-wet (θ = 94° ± 13°) homogeneous micromodels under four different flow rates (capillary number Ca ranging from 9 × 10-6 to 8 × 10-4) with a total of eight conditions (four replicates for each). Brine invasion processes were recorded and statistical analysis was performed for over 2000 images of scCO2 saturations, and scCO2 cluster characteristics. The trapped scCO2 saturation under intermediate-wet conditions is 15% higher than under water-wet conditions under the slowest flow rate (Ca ˜ 9 × 10-6). Based on the visualization and scCO2 cluster analysis, we show that the scCO2 trapping process in our micromodels is governed by bypass trapping that is enhanced by the larger contact angle. Smaller contact angles enhance cooperative pore filling and widen brine fingers (or channels), leading to smaller volumes of scCO2 being bypassed. Increased flow rates suppress this wettability effect.

Effect of surfactant on kinetics of thinning of capillary bridges

NASA Astrophysics Data System (ADS)

Nowak, Emilia; Kovalchuk, Nina; Simmons, Mark

2015-11-01

Kinetics of thinning of capillary bridges is of great scientific and industrial interest being of vital importance for example in various emulsification and microfluidic processes. It is well known that the rate of bridge thinning is proportional to the interfacial tension. Therefore it is expected that the process should slow down by addition of surfactant. The kinetics of capillary bridges in the presence of surfactant was studied by the dripping of liquid from a capillary tip under conditions of nearly zero flow rate (We << 1). The tested liquids were aqueous solutions of sodium lauryl ether sulphate (SLES), which is broadly used in personal care products. The viscosity, surfactant activity and adsorption kinetics have been controlled by addition of glycerol and sodium chloride. The study has shown that the kinetics of capillary bridges are determined by dynamic surface tension rather than by its equilibrium value. In particular, the kinetics of the bridge thinning for the 0.1 g L-1 aqueous SLES solution is practically the same as that of pure water despite twice lower equilibrium surface tension. EPSRC Programme Grant, MEMPHIS, EP/K0039761/1.

Apparatus for characterizing the temporo-spatial properties of a dynamic fluid front and method thereof

DOEpatents

Battiste, Richard L.

2007-12-25

Methods and apparatus are described for characterizing the temporal-spatial properties of a dynamic fluid front within a mold space while the mold space is being filled with fluid. A method includes providing a mold defining a mold space and having one or more openings into the mold space; heating a plurality of temperature sensors that extend into the mold space; injecting a fluid into the mold space through the openings, the fluid experiencing a dynamic fluid front while filling the mold space with the fluid; and characterizing temporal-spatial properties of the dynamic fluid front by monitoring a temperature of each of the plurality of heated temperature sensors while the mold space is being filled with the fluid. An apparatus includes a mold defining a mold space; one or more openings for introducing a fluid into the mold space and filling the mold space with the fluid, the fluid experiencing a dynamic fluid front while filling the mold space; a plurality of heated temperature sensors extending into the mold space; and a computer coupled to the plurality of heated temperature sensors for characterizing the temporal-spatial properties of the dynamic fluid front.

Apparatus for characterizing the temporo-spatial properties of a dynamic fluid front and method thereof

DOEpatents

Battiste, Richard L

2013-12-31

Methods and apparatus are described for characterizing the temporal-spatial properties of a dynamic fluid front within a mold space while the mold space is being filled with fluid. A method includes providing a mold defining a mold space and having one or more openings into the mold space; heating a plurality of temperature sensors that extend into the mold space; injecting a fluid into th emold space through the openings, the fluid experiencing a dynamic fluid front while filling the mold space with a fluid; and characterizing temporal-spatial properties of the dynamic fluid front by monitoring a termperature of each of the plurality of heated temperature sensors while the mold space is being filled with the fluid. An apparatus includes a mold defining a mold space; one or more openings for introducing a fluid into th emold space and filling the mold space with the fluid, the fluid experiencing a dynamic fluid front while filling the mold space; a plurality of heated temperature sensors extending into the mold space; and a computer coupled to the plurality of heated temperature sensors for characterizing the temporal-spatial properties of the dynamic fluid front.

TOPICAL REVIEW Recent developments in inorganically filled carbon nanotubes: successes and challenges

NASA Astrophysics Data System (ADS)

Gautam, Ujjal K.; Costa, Pedro M. F. J.; Bando, Yoshio; Fang, Xiaosheng; Li, Liang; Imura, Masataka; Golberg, Dmitri

2010-10-01

Carbon nanotubes (CNTs) are a unique class of nanomaterials that can be imagined as rolled graphene sheets. The inner hollow of a CNT provides an extremely small, one-dimensional space for storage of materials. In the last decade, enormous effort has been spent to produce filled CNTs that combine the properties of both the host CNT and the guest filling material. CNTs filled with various inorganic materials such as metals, alloys, semiconductors and insulators have been obtained using different synthesis approaches including capillary filling and chemical vapor deposition. Recently, several potential applications have emerged for these materials, such as the measurement of temperature at the nanoscale, nano-spot welding, and the storage and delivery of extremely small quantities of materials. A clear distinction between this class of materials and other nanostructures is the existence of an enormous interfacial area between the CNT and the filling matter. Theoretical investigations have shown that the lattice mismatch and strong exchange interaction of CNTs with the guest material across the interface should result in reordering of the guest crystal structure and passivation of the surface dangling bonds and thus yielding new and interesting physical properties. Despite preliminary successes, there remain many challenges in realizing applications of CNTs filled with inorganic materials, such as a comprehensive understanding of their growth and physical properties and control of their structural parameters. In this article, we overview research on filled CNT nanomaterials with special emphasis on recent progress and key achievements. We also discuss the future scope and the key challenges emerging out of a decade of intensive research on these fascinating materials.

Left atrial strain: a new parameter for assessment of left ventricular filling pressure.

PubMed

Cameli, Matteo; Mandoli, Giulia Elena; Loiacono, Ferdinando; Dini, Frank Lloyd; Henein, Michael; Mondillo, Sergio

2016-01-01

In order to obtain accurate diagnosis, treatment and prognostication in many cardiac conditions, there is a need for assessment of left ventricular (LV) filling pressure. While systole depends on ejection function of LV, diastole and its disturbances influence filling function and pressures. The commonest condition that represents the latter is heart failure with preserved ejection fraction in which LV ejection is maintained, but diastole is disturbed and hence filling pressures are raised. Significant diastolic dysfunction results in raised LV end-diastolic pressure, mean left atrial (LA) pressure and pulmonary capillary wedge pressure, all referred to as LV filling pressures. Left and right heart catheterization has traditionally been used as the gold standard investigation for assessing these pressures. More recently, Doppler echocardiography has taken over such application because of its noninvasive nature and for being patient friendly. A number of indices are used to achieve accurate assessment of filling pressures including: LV pulsed-wave filling velocities (E/A ratio, E wave deceleration time), pulmonary venous flow (S wave and D wave), tissue Doppler imaging (E' wave and E/E' ratio) and LA volume index. LA longitudinal strain derived from speckle tracking echocardiography (STE) is also sensitive in estimating intracavitary pressures. It is angle-independent, thus overcomes Doppler limitations and provides highly reproducible measures of LA deformation. This review examines the application of various Doppler echocardiographic techniques in assessing LV filling pressures, in particular the emerging role of STE in assessing LA pressures in various conditions, e.g., HF, arterial hypertension and atrial fibrillation.

Stability limits of unsteady open capillary channel flow

NASA Astrophysics Data System (ADS)

Grah, Aleksander; Haake, Dennis; Rosendahl, Uwe; Klatte, J.?Rg; Dreyer, Michael E.

This paper is concerned with steady and unsteady flow rate limitations in open capillary channels under low-gravity conditions. Capillary channels are widely used in Space technology for liquid transportation and positioning, e.g. in fuel tanks and life support systems. The channel observed in this work consists of two parallel plates bounded by free liquid surfaces along the open sides. The capillary forces of the free surfaces prevent leaking of the liquid and gas ingestion into the flow.In the case of steady stable flow the capillary pressure balances the differential pressure between the liquid and the surrounding constant-pressure gas phase. Increasing the flow rate in small steps causes a decrease of the liquid pressure. A maximum steady flow rate is achieved when the flow rate exceeds a certain limit leading to a collapse of the free surfaces due to the choking effect. In the case of unsteady flow additional dynamic effects take place due to flow rate transition and liquid acceleration. The maximum flow rate is smaller than in the case of steady flow. On the other hand, the choking effect does not necessarily cause surface collapse and stable temporarily choked flow is possible under certain circumstances.To determine the limiting volumetric flow rate and stable flow dynamic properties, a new stability theory for both steady and unsteady flow is introduced. Subcritical and supercritical (choked) flow regimes are defined. Stability criteria are formulated for each flow type. The steady (subcritical) criterion corresponds to the speed index defined by the limiting longitudinal small-amplitude wave speed, similar to the Mach number. The unsteady (supercritical) criterion for choked flow is defined by a new characteristic number, the dynamic index. It is based on pressure balances and reaches unity at the stability limit.The unsteady model based on the Bernoulli equation and the mass balance equation is solved numerically for perfectly wetting incompressible liquids. The unsteady model and the stability theory are verified by comparison to results of a sounding rocket experiment (TEXUS 41) on capillary channel flows launched in December 2005 from ESRANGE in north Sweden. For a clear overview of subcritical, supercritical, and unstable flow, parametric studies and stability diagrams are shown and compared to experimental observations.

Accretion Dynamics on Wet Granular Materials

NASA Astrophysics Data System (ADS)

Saingier, Guillaume; Sauret, Alban; Jop, Pierre

2017-05-01

Wet granular aggregates are common precursors of construction materials, food, and health care products. The physical mechanisms involved in the mixing of dry grains with a wet substrate are not well understood and difficult to control. Here, we study experimentally the accretion of dry grains on a wet granular substrate by measuring the growth dynamics of the wet aggregate. We show that this aggregate is fully saturated and its cohesion is ensured by the capillary depression at the air-liquid interface. The growth dynamics is controlled by the liquid fraction at the surface of the aggregate and exhibits two regimes. In the viscous regime, the growth dynamics is limited by the capillary-driven flow of liquid through the granular packing to the surface of the aggregate. In the capture regime, the capture probability depends on the availability of the liquid at the saturated interface, which is controlled by the hydrostatic depression in the material. We propose a model that rationalizes our observations and captures both dynamics based on the evolution of the capture probability with the hydrostatic depression.

Comparison of HPLC and CE for the analysis of dichlorprop in a case of intoxication.

PubMed

West, A; Frost, M; Köhler, H

1997-01-01

A 49-year-old white male was found lying unconscious at home. He had vomited, his mouth was filled with a white foam and a pungent odour filled the room. After emergency treatment blood, urine and stomach contents were screened for drugs after acid and alkaline extraction with subsequent derivatisation and GC-MS analysis. Large quantites of 2,4-dichlorophenoxypropionic acid (dichlorprop, 2,4-DP) were found in all acid extracts. The man died 3 h later in hospital. Body fluids and tissues obtained at autopsy were analysed for 2,4-DP by high-performance liquid chromatography and capillary electrophoresis. The concentrations of 2,4-DP in cardiac blood, stomach contents, bile, liver, spleen, kidney and brain found by both methods were very similar.

Dynamics of liquid spreading on solid surfaces

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kalliadasis, S.; Chang, H.C.

1996-09-01

Using simple scaling arguments and a precursor film model, the authors show that the appropriate macroscopic contact angle {theta} during the slow spreading of a completely or partially wetting liquid under conditions of viscous flow and small slopes should be described by tan {theta} = [tan{sup 3} {theta}{sub e} {minus} 9 log {eta}Ca]{sup 1/3} where {theta}{sub e} is the static contact angle, Ca is the capillary number, and {eta} is a scaled Hamaker constant. Using this simple relation as a boundary condition, the authors are able to quantitatively model, without any empirical parameter, the spreading dynamics of several classical spreadingmore » phenomena (capillary rise, sessile, and pendant drop spreading) by simply equating the slope of the leading order static bulk region to the dynamic contact angle boundary condition without performing a matched asymptotic analysis for each case independently as is usually done in the literature.« less

Accuracy of the HumaSensplus point-of-care uric acid meter using capillary blood obtained by fingertip puncture.

PubMed

Fabre, Stéphanie; Clerson, Pierre; Launay, Jean-Marie; Gautier, Jean-François; Vidal-Trecan, Tiphaine; Riveline, Jean-Pierre; Platt, Adam; Abrahamsson, Anna; Miner, Jeffrey N; Hughes, Glen; Richette, Pascal; Bardin, Thomas

2018-05-02

The uric acid (UA) level in patients with gout is a key factor in disease management and is typically measured in the laboratory using plasma samples obtained after venous puncture. This study aimed to assess the reliability of immediate UA measurement with capillary blood samples obtained by fingertip puncture with the HumaSens plus point-of-care meter. UA levels were measured using both the HumaSens plus meter in the clinic and the routine plasma UA method in the biochemistry laboratory of 238 consenting diabetic patients. HumaSens plus capillary and routine plasma UA measurements were compared by linear regression, Bland-Altman plots, intraclass correlation coefficient (ICC), and Lin's concordance coefficient. Values outside the dynamic range of the meter, low (LO) or high (HI), were analyzed separately. The best capillary UA thresholds for detecting hyperuricemia were determined by receiver operating characteristic (ROC) curves. The impact of potential confounding factors (demographic and biological parameters/treatments) was assessed. Capillary and routine plasma UA levels were compared to reference plasma UA measurements by liquid chromatography-mass spectrometry (LC-MS) for a subgroup of 67 patients. In total, 205 patients had capillary and routine plasma UA measurements available. ICC was 0.90 (95% confidence interval (CI) 0.87-0.92), Lin's coefficient was 0.91 (0.88-0.93), and the Bland-Altman plot showed good agreement over all tested values. Overall, 17 patients showed values outside the dynamic range. LO values were concordant with plasma values, but HI values were considered uninterpretable. Capillary UA thresholds of 299 and 340 μmol/l gave the best results for detecting hyperuricemia (corresponding to routine plasma UA thresholds of 300 and 360 μmol/l, respectively). No significant confounding factor was found among those tested, except for hematocrit; however, this had a negligible influence on the assay reliability. When capillary and routine plasma results were discordant, comparison with LC-MS measurements showed that plasma measurements had better concordance: capillary UA, ICC 0.84 (95% CI 0.75-0.90), Lin's coefficient 0.84 (0.77-0.91); plasma UA, ICC 0.96 (0.94-0.98), Lin's coefficient 0.96 (0.94-0.98). UA measurements with the HumaSens plus meter were reasonably comparable with those of the laboratory assay. The meter is easy to use and may be useful in the clinic and in epidemiologic studies.

The Soil Foam Drainage Equation - an alternative model for unsaturated flow in porous media

NASA Astrophysics Data System (ADS)

Assouline, Shmuel; Lehmann, Peter; Hoogland, Frouke; Or, Dani

2017-04-01

The analogy between the geometry and dynamics of wet foam drainage and gravity drainage of unsaturated porous media expands modeling capabilities for capillary flows and supplements the standard Richards equation representation. The governing equation for draining foam (or a soil variant termed the soil foam drainage equation - SFDE) obviates the need for macroscopic unsaturated hydraulic conductivity function by an explicit account of diminishing flow pathway sizes as the medium gradually drains. Potential advantages of the proposed drainage foam formalism include direct description of transient flow without requiring constitutive functions; evolution of capillary cross sections that provides consistent description of self-regulating internal fluxes (e.g., towards field capacity); and a more intuitive geometrical picture of capillary flow across textural boundaries. We will present new and simple analytical expressions for drainage rates and volumes from unsaturated porous media subjected to different boundary conditions that are in good agreement with the numerical solution of the SFDE and experimental results. The foam drainage methodology expands the range of tools available for describing and quantifying unsaturated flows and provides geometrically tractable links between evolution of liquid configuration and flow dynamics in unsaturated porous media. The resulting geometrical representation of capillary drainage could improve understanding of colloid and pathogen transport. The explicit geometrical interpretation of flow pathways underlying the hydraulic functions used by the Richards equation offers new insights that benefit both approaches.

Recombination-pumped XUV lasing in capillary discharges and dynamic z-pinches

NASA Astrophysics Data System (ADS)

Pöckl, M.; Hebenstreit, M.; Fertner, R.; Neger, T.; Aumayr, F.

1996-08-01

A fully time-dependent collisional - radiative model is employed to calculate relevant population densities in a recombining carbon/hydrogen z-pinch plasma. In particular, the dependence of the small signal gain G on the maximum electron temperature and cooling rate, as well as the influence of Lyman-0022-3727/29/8/005/img8 reabsorption, are studied. Although in conditions typical for dynamic z-pinches the maximum electron temperature and cooling rates would, in principle, be sufficiently high, gain on the Balmer-0022-3727/29/8/005/img8 transition is strongly reduced by Lyman-0022-3727/29/8/005/img8 reabsorption. In order to investigate vacuum spark capillary discharges, the system of rate equations is coupled with balance equations of the plasma energy and the total number of heavy particles. The resulting set of equations is solved self-consistently. Results are presented that show the systematic dependence of the small signal gain on electrical input power, wall material, and capillary geometry. High gain coefficients 0022-3727/29/8/005/img11 could be achieved by modelling high-voltage discharges with short ringing periods through capillaries containing boron or carbon. While the maximum achievable gain coefficient for lithium is rather poor 0022-3727/29/8/005/img12 the duration of population inversion would be long enough (a few tens of nanoseconds) to make multi-pass operation possible.

Interfacial layering and capillary roughness in immiscible liquids.

PubMed

Geysermans, P; Pontikis, V

2010-08-21

The capillary roughness and the atomic density profiles of extended interfaces between immiscible liquids are determined as a function of the interface area by using molecular dynamics and Lennard-Jones (12-6) potentials. We found that with increasing area, the interface roughness diverges logarithmically, thus fitting the theoretical mean-field prediction. In systems small enough for the interfacial roughness not to blur the structural details, atomic density profiles across the fluid interface are layered with correlation length in the range of molecular correlations in liquids. On increasing the system size, the amplitude of the thermally excited position fluctuations of the interface increases, thus causing layering to rapidly vanish, if density profiles are computed without special care. In this work, we present and validate a simple method, operating in the direct space, for extracting from molecular dynamics trajectories the "intrinsic" structure of a fluid interface that is the local density profile of the interface cleaned from capillary wave effects. Estimated values of interfacial properties such as the tension, the intrinsic width, and the lower wavelength limit of position fluctuations are in agreement with results collected from the literature.

Lymphocyte migration in the micro-channel of splenic sheathed capillaries in Chinese soft-shelled turtles, Pelodiscus sinensis.

PubMed

Zhang, Qian; Ullah, Shakeeb; Liu, Yi; Yang, Ping; Chen, Bing; Waqas, Yasir; Bao, Huijun; Hu, Lisi; Li, Quanfu; Chen, Qiusheng

2016-01-01

The structural characteristics of the splenic sheathed capillary were investigated using light microscopy and transmission electron microscopy (TEM). This study mainly focused on lymphocyte migration to the splenic white pulp via micro-channels in Chinese soft-shelled turtles, Pelodiscus sinensis. The results showed that the sheathed capillaries in the turtle spleen were high endothelial venule (HEV)-like vessels. These capillaries consist of micro-channels that facilitate lymphocyte migration to the splenic white pulp. The micro-channel is a dynamic structure comprising processes of endothelial cells, supporting cells, and ellipsoid-associated cells (EACs), which provides a microenvironment for lymphocyte migration. The pattern of lymphocyte migration in the micro-channel of the turtle spleen includes the following steps: (i) lymphocyte first adheres to the endothelium of the sheathed capillary, passes through the endothelial cells, and traverses through the basement membrane of the sheathed capillary; (ii) it then enters into the ellipsoid combined with supporting cells and EACs; and (iii) lymphocyte migrates from the ellipsoid to the periellipsoidal lymphatic sheath (PELS) via the micro-channel. This study provides morphological evidence for lymphocyte migration in the micro-channels of turtle spleens and also an insight into the mechanism of lymphocyte homing to the splenic white pulp of reptiles. Copyright © 2015 Elsevier Ltd. All rights reserved.

Correlating contact line capillarity and dynamic contact angle hysteresis in surfactant-nanoparticle based complex fluids

NASA Astrophysics Data System (ADS)

Harikrishnan, A. R.; Dhar, Purbarun; Agnihotri, Prabhat K.; Gedupudi, Sateesh; Das, Sarit K.

2018-04-01

Dynamic wettability and contact angle hysteresis can be correlated to shed insight onto any solid-liquid interaction. Complex fluids are capable of altering the expected hysteresis and dynamic wetting behavior due to interfacial interactions. We report the effect of capillary number on the dynamic advancing and receding contact angles of surfactant-based nanocolloidal solutions on hydrophilic, near hydrophobic, and superhydrophobic surfaces by performing forced wetting and de-wetting experiments by employing the embedded needle method. A segregated study is performed to infer the contributing effects of the constituents and effects of particle morphology. The static contact angle hysteresis is found to be a function of particle and surfactant concentrations and greatly depends on the nature of the morphology of the particles. An order of estimate of line energy and a dynamic flow parameter called spreading factor and the transient variations of these parameters are explored which sheds light on the dynamics of contact line movement and response to perturbation of three-phase contact. The Cox-Voinov-Tanner law was found to hold for hydrophilic and a weak dependency on superhydrophobic surfaces with capillary number, and even for the complex fluids, with a varying degree of dependency for different fluids.

Effects of intermediate wettability on entry capillary pressure in angular pores.

PubMed

Rabbani, Harris Sajjad; Joekar-Niasar, Vahid; Shokri, Nima

2016-07-01

Entry capillary pressure is one of the most important factors controlling drainage and remobilization of the capillary-trapped phases as it is the limiting factor against the two-phase displacement. It is known that the entry capillary pressure is rate dependent such that the inertia forces would enhance entry of the non-wetting phase into the pores. More importantly the entry capillary pressure is wettability dependent. However, while the movement of a meniscus into a strongly water-wet pore is well-defined, the invasion of a meniscus into a weak or intermediate water-wet pore especially in the case of angular pores is ambiguous. In this study using OpenFOAM software, high-resolution direct two-phase flow simulations of movement of a meniscus in a single capillary channel are performed. Interface dynamics in angular pores under drainage conditions have been simulated under constant flow rate boundary condition at different wettability conditions. Our results shows that the relation between the half corner angle of pores and contact angle controls the temporal evolution of capillary pressure during the invasion of a pore. By deviating from pure water-wet conditions, a dip in the temporal evolution of capillary pressure can be observed which will be pronounced in irregular angular cross sections. That enhances the pore invasion with a smaller differential pressure. The interplay between the contact angle and pore geometry can have significant implications for enhanced remobilization of ganglia in intermediate contact angles in real porous media morphologies, where pores are very heterogeneous with small shape factors. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

The Capillary Flow Experiments Aboard the International Space Station: Increments 9-15

NASA Technical Reports Server (NTRS)

Jenson, Ryan M.; Weislogel, Mark M.; Tavan, Noel T.; Chen, Yongkang; Semerjian, Ben; Bunnell, Charles T.; Collicott, Steven H.; Klatte, Jorg; dreyer, Michael E.

2009-01-01

This report provides a summary of the experimental, analytical, and numerical results of the Capillary Flow Experiment (CFE) performed aboard the International Space Station (ISS). The experiments were conducted in space beginning with Increment 9 through Increment 16, beginning August 2004 and ending December 2007. Both primary and extra science experiments were conducted during 19 operations performed by 7 astronauts including: M. Fincke, W. McArthur, J. Williams, S. Williams, M. Lopez-Alegria, C. Anderson, and P. Whitson. CFE consists of 6 approximately 1 to 2 kg handheld experiment units designed to investigate a selection of capillary phenomena of fundamental and applied importance, such as large length scale contact line dynamics (CFE-Contact Line), critical wetting in discontinuous structures (CFE-Vane Gap), and capillary flows and passive phase separations in complex containers (CFE-Interior Corner Flow). Highly quantitative video from the simply performed flight experiments provide data helpful in benchmarking numerical methods, confirming theoretical models, and guiding new model development. In an extensive executive summary, a brief history of the experiment is reviewed before introducing the science investigated. A selection of experimental results and comparisons with both analytic and numerical predictions is given. The subsequent chapters provide additional details of the experimental and analytical methods developed and employed. These include current presentations of the state of the data reduction which we anticipate will continue throughout the year and culminate in several more publications. An extensive appendix is used to provide support material such as an experiment history, dissemination items to date (CFE publication, etc.), detailed design drawings, and crew procedures. Despite the simple nature of the experiments and procedures, many of the experimental results may be practically employed to enhance the design of spacecraft engineering systems involving capillary interface dynamics.

Water Displacement in Oil-Wet Tight Reservoirs by Dynamic Network Simulation

NASA Astrophysics Data System (ADS)

Wang, Y.; Li, M.; Chen, M.

2017-12-01

Pore network simulation is an effective tool for studying the multiphase flow in porous media. Based on the topological information and pore-throat size distribution obtained from the analysis of Scanning Electron Microscope (SEM) and constant-rate mercury injection (CRMI) for tight cores (composed by micro-nano scale throats and micro scale pores), a simple cubic (SC) pore-throat network was built with equilateral triangular cross-section throats and cubic bodies. Rules for oil and water movement and redistribution were devised in accordance with the physics process at pore-throat scale. Water flooding from oil-saturated under irreducible water were simulated by considering the changing displacement rate and viscosity ratio at the slightly oil-wet condition (the static contact angle ranges between π/2 to 2π/3). Different from the double pressure field algorithm, a single pressure field which solved by using successive over relaxation method was used with the flow of irreducible water in corners was ignored while its swilling was take into consideration. Dynamic of displacement fronts, relative permeability curves and residual oil saturation were obtained. It showed that there were obviously snap-off at low capillary number (Nc<10-5) and fingering at high capillary number (Nc<10-4) even at a favorable viscosity ratio (M=1). The magnitude of viscosity ratio effect on relative permeability depended largely on the capillary number, which the effect wasn't noticeable for a high capillary number. For residual oil saturation Sor, it showed that Sor decreased with the increase of capillary number at different viscosity ratio. Changing of residual oil saturation from simulation was in good agreement with the experimental results in a certain range, which indicated that this network model could be used to character the water flooding in tight reservoirs.

Laser-driven acceleration of electrons in a partially ionized plasma channel.

PubMed

Rowlands-Rees, T P; Kamperidis, C; Kneip, S; Gonsalves, A J; Mangles, S P D; Gallacher, J G; Brunetti, E; Ibbotson, T; Murphy, C D; Foster, P S; Streeter, M J V; Budde, F; Norreys, P A; Jaroszynski, D A; Krushelnick, K; Najmudin, Z; Hooker, S M

2008-03-14

The generation of quasimonoenergetic electron beams, with energies up to 200 MeV, by a laser-plasma accelerator driven in a hydrogen-filled capillary discharge waveguide is investigated. Injection and acceleration of electrons is found to depend sensitively on the delay between the onset of the discharge current and the arrival of the laser pulse. A comparison of spectroscopic and interferometric measurements suggests that injection is assisted by laser ionization of atoms or ions within the channel.

Multifunctional Poro-Vascular Composites for UAV Performance Enhancement

DTIC Science & Technology

2012-07-31

structural “skin” materials with surface pores and internal vascular channels filled with an ionic- liquid whose height and shape at the pore exits is...2 V t e e q q          Intrinsic contact angle (zero voltage) Applied potential Interfacial tension (IFT) of ionic liquid Lippmann...Characterization flat plates  single (capillary) pore  PV pore arrays FTA 1000 Drop-Shape Characterization • Microscope lens : 0.5 to 12x magnification • Side

Realizing A Mid-Infrared Optically Pumped Molecular Gas Laser Inside Hollow-Core Photonic Crystal Fiber

DTIC Science & Technology

2012-01-01

54  Figure 5.2 External transmission of (a) BK7 glass , (b) CaF2, and (c) Germanium substrates of various thicknesses. The transmission values...a hollow, air-filled core surrounded by cladding structure which is comprised of a periodic array of smaller air holes. Unlike glass capillaries...region of the fiber’s core. The low overlap of the guided light with the HC-PCF’s glass structure together with a higher threshold for the onset of

Development in electrophoresis: instrumentation for two-dimensional gel electrophoresis of protein separation and application of capillary electrophoresis in micro-bioanalysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, Aoshuang

2008-01-01

This dissertation begins with a general introduction of topics related to this work. The following chapters contain three scientific manuscripts, each presented in a separate chapter with accompanying tables, figures, and literature citations. The final chapter summarizes the work and provides some prospective on this work. This introduction starts with a brief treatment of the basic principles of electrophoresis separation, followed by a discussion of gel electrophoresis and particularly polyacrylamide gel electrophoresis for protein separation, a summary of common capillary electrophoresis separation modes, and a brief treatment of micro-bioanalysis application of capillary electrophoresis, and ends with an overview of proteinmore » conformation and dynamics.« less

Single particles accelerate final stages of capillary break-up

NASA Astrophysics Data System (ADS)

Lindner, Anke; Fiscina, Jorge Eduardo; Wagner, Christian

2015-06-01

Droplet formation of suspensions is present in many industrial and technological processes such as coating and food engineering. Whilst the finite-time singularity of the minimum neck diameter in capillary break-up of simple liquids can be described by well-known self-similarity solutions, the pinching of non-Brownian suspension depends in a complex way on the particle dynamics in the thinning thread. Here we focus on the very dilute regime where the filament contains only isolated beads to identify the physical mechanisms leading to the pronounced acceleration of the filament thinning observed. This accelerated regime is characterized by an asymmetric shape of the filament with an enhanced curvature that depends on the size and the spatial distribution of the particles within the capillary thread.

Adsorption detection for polylysine biomolecules based on high-Q silica capillary whispering gallery mode microresonator

NASA Astrophysics Data System (ADS)

Wu, Jixuan; Liu, Bo; Zhang, Hao; Song, Binbin

2017-11-01

A silica-capillary-based whispering gallery mode (WGM) microresonator has been proposed and experimentally demonstrated for the real-time monitoring of the polylysine adsorption process. The spectral characteristics of the WGM resonance dips with high quality factor and good wavelength selectivity have been investigated to evaluate the dynamic process for the binding of polylysine with a capillary surface. The WGM transmission spectrum shows a regular shift with increments of observation time, which could be exploited for the analysis of the polylysine adsorption process. The proposed WGM microresonator system possesses desirable qualities such as high sensitivity, fast response, label-free method, high detection resolution and compactness, which could find promising applications in histology and related bioengineering areas.

Capillary rise and swelling in cellulose sponges

NASA Astrophysics Data System (ADS)

Ha, Jonghyun; Kim, Jungchul; Kim, Ho-Young

2015-11-01

A cellulose sponge, which is a mundane example of a porous hydrophilic structure, can absorb and hold a significant amount of liquid. We present the results of experimental and theoretical investigation of the dynamics of the capillary imbibition of various aqueous solutions in the sponge that swells at the same time. We find that the rate of water rise against the resistance caused by gravitational and viscous effects deviates from Washburn's rule beyond a certain threshold height. We rationalize the novel power law of the rise height versus time by combining Darcy's law with hygroscopic swelling equation and also predict the threshold height. The scaling law constructed through this work agrees well with the experimental results, shedding light on the physics of capillary flow in deforming porous media.

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014-2016).

PubMed

Breadmore, Michael C; Wuethrich, Alain; Li, Feng; Phung, Sui Ching; Kalsoom, Umme; Cabot, Joan M; Tehranirokh, Masoomeh; Shallan, Aliaa I; Abdul Keyon, Aemi S; See, Hong Heng; Dawod, Mohamed; Quirino, Joselito P

2017-01-01

One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biennial reviews, first published in Electrophoresis in 2007, on developments in the field of on-line/in-line concentration methods in capillaries and microchips, covering the period July 2014-June 2016. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction, and sweeping. Attention is also given to on-line or in-line extraction methods that have been used for electrophoresis. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Bubble deformations and segmented flows in corrugated microchannels at large capillary numbers

NASA Astrophysics Data System (ADS)

Sauzade, Martin; Cubaud, Thomas

2018-03-01

We experimentally investigate the interaction between individual bubble deformations and collective distortions of segmented flows in nonlinear microfluidic geometries. Using highly viscous carrier fluids, we study the evolution of monodisperse trains of gas bubbles from a square to a smoothly corrugated microchannel characterized with a series of extensions and constrictions along the flow path. The hysteresis in the bubble shape between accelerating and decelerating flow fields is shown to increase with the capillary number. Measurements of instantaneous bubble velocities reveal the presence of a capillary pull that produces a nonmonotonic behavior for the front velocity in accelerating flow regions. Functional relationships are developed for predicting the morphology and dynamics of viscous multiphase flow patterns at the pore scale.

Theoretical and experimental separation dynamics in capillary zone electrophoresis

NASA Technical Reports Server (NTRS)

Thormann, Wolfgang; Michaud, Jon-Pierre; Mosher, Richard A.

1986-01-01

The mathematical model of Bier et al. (1983) is used in a computer aided analysis of the conditions in capillary zone electrophoresis (ZE) under which sample zones migrate noninteractively with the carrier electrolyte. The monitoring of sample zones with a capillary analyzer that features both on-line conductivity and UV detection at the end of the separation trough is discussed. Data from a ZE analysis of a 5-component mixture are presented, and it is noted that all five components can be monitored via their conductivity change if enough sample is present. It is suggested from the results that the concentration ratio of background buffer to sample should be a minimum of 100:1 to effectively apply the plate concept to ZE.

On-line focusing of flavin derivatives using Dynamic pH junction-sweeping capillary electrophoresis with laser-induced fluorescence detection.

PubMed

Britz-McKibbin, Philip; Otsuka, Koji; Terabe, Shigeru

2002-08-01

Simple yet effective methods to enhance concentration sensitivity is needed for capillary electrophoresis (CE) to become a practical method to analyze trace levels of analytes in real samples. In this report, the development of a novel on-line preconcentration technique combining dynamic pH junction and sweeping modes of focusing is applied to the sensitive and selective analysis of three flavin derivatives: riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Picomolar (pM) detectability of flavins by CE with laser-induced fluorescence (LIF) detection is demonstrated through effective focusing of large sample volumes (up to 22% capillary length) using a dual pH junction-sweeping focusing mode. This results in greater than a 1,200-fold improvement in sensitivity relative to conventional injection methods, giving a limit of detection (S/N = 3) of approximately 4.0 pM for FAD and FMN. Flavin focusing is examined in terms of analyte mobility dependence on buffer pH, borate complexation and SDS interaction. Dynamic pH junction-sweeping extends on-line focusing to both neutral (hydrophobic) and weakly acidic (hydrophilic) species and is considered useful in cases when either conventional sweeping or dynamic pH junction techniques used alone are less effective for certain classes of analytes. Enhanced focusing performance by this hyphenated method was demonstrated by greater than a 4-fold reduction in flavin bandwidth, as compared to either sweeping or dynamic pH junction, reflected by analyte detector bandwidths <0.20 cm. Novel on-line focusing strategies are required to improve sensitivity in CE, which may be applied toward more effective biochemical analysis methods for diverse types of analytes.

Enabling two-phase microfluidic thermal transport systems using a novel thermal-flux degassing and fluid charging approach

NASA Astrophysics Data System (ADS)

Singh Dhillon, Navdeep; Pisano, Albert P.

2014-03-01

A novel two-port thermal-flux method has been proposed and demonstrated for degassing and charging two-phase microfluidic thermal transport systems with a degassed working fluid. In microscale heat pipes and loop heat pipes (mLHPs), small device volumes and large capillary forces associated with smaller feature sizes render conventional vacuum pump-based degassing methods quite impractical. Instead, we employ a thermally generated pressure differential to purge non-condensable gases from these devices before charging them with a degassed working fluid in a two-step process. Based on the results of preliminary experiments studying the effectiveness and reliability of three different high temperature-compatible device packaging approaches, an optimized compression packaging technique was developed to degas and charge a mLHP device using the thermal-flux method. An induction heating-based noninvasive hermetic sealing approach for permanently sealing the degassed and charged mLHP devices has also been proposed. To demonstrate the efficacy of this approach, induction heating experiments were performed to noninvasively seal 1 mm square silicon fill-hole samples with donut-shaped solder preforms. The results show that the minimum hole sealing induction heating time is heat flux limited and can be estimated using a lumped capacitance thermal model. However, further continued heating of the solder uncovers the hole due to surface tension-induced contact line dynamics of the molten solder. It was found that an optimum mass of the solder preform is required to ensure a wide enough induction-heating time window for successful sealing of a fill-hole.

Quantitative evaluation of capillaroscopic microvascular changes in patients with established coronary heart disease.

PubMed

Sanchez-Garcia, M Esther; Ramirez-Lara, Irene; Gomez-Delgado, Francisco; Yubero-Serrano, Elena M; Leon-Acuña, Ana; Marin, Carmen; Alcala-Diaz, Juan F; Camargo, Antonio; Lopez-Moreno, Javier; Perez-Martinez, Pablo; Tinahones, Francisco José; Ordovas, Jose M; Caballero, Javier; Blanco-Molina, Angeles; Lopez-Miranda, Jose; Delgado-Lista, Javier

2018-02-23

Microcirculation disturbances have been associated to most of the cardiovascular risk factors as well as to multiple inflammatory diseases. However, whether these abnormalities are specifically augmented in patients with coronary heart disease is still unknown. We aimed to evaluate if there is a relationship between the presence of coronary heart disease and the existence of functional and structural capillary abnormalities evaluated in the cutaneous microcirculation by videocapillaroscopy. Two matched samples of 30 participants with and without coronary heart disease but with similar clinical and anthropometric characteristics were evaluated by videocapillaroscopy at the dorsal skin of the third finger of the non-dominant hand. We calculated basal capillary density as well as capillary density after a period of arterial and venous occlusion in order to evaluate functionality and maximum capillary density. We also measured capillary recruitment. Microvascular capillary density at rest was significantly lower in patients suffering from coronary heart disease than in controls. This fact was also found after dynamic tests (arterial and venous occlusion), suggesting functional impairments. Capillary recruitment of the samples was not different in our sample. In our study, patients with coronary heart disease exhibit functional and structural microvascular disturbances. Although this is a very preliminary study, these findings open the door for further studying the microvascular functionality in coronary patients and how it relates to the response to treatment and/or the prognosis of the disease. Copyright © 2017 Elsevier España, S.L.U. All rights reserved.

Numerical simulation of ion transport in an atmosphere-to-vacuum interface taking into account gas dynamics and space charge.

PubMed

Skoblin, Michael G; Chudinov, Alexey V; Sulimenkov, Ilia V; Brusov, Vladimir S; Makarov, Alexander A; Wouters, Eloy R; Kozlovskiy, Viacheslav I

2017-08-01

A two-step approach was developed for the study of ion transport in an atmospheric pressure interface. In the first step, the flow in the interface was numerically simulated using the standard gas dynamic package ANSYS CFX 15.0. In the second step, the calculated fields of pressure, temperature, and velocity were imported into a custom-built software application for simulation of ion motion under the influence of both gas dynamic and electrostatic forces. To account for space charge effects in axially symmetric interfaces an analytical expression was used for the Coulomb force. For all other types of interfaces, an iterative approach for the Coulomb force computation was developed. The simulations show that the influence of the space charge is the main contributor to the loss of ion current in the heated capillary. In addition, the maximum ion current which can be transmitted through the heated capillary (0.58 mm inner diameter and 58.5 mm length) is limited to ∼6 nA for ions with m/z = 508 Da and with reduced ion mobility 1.05 cm 2 V -1 s -1 . This limit remains practically constant and independent of the ion current at the entrance of the capillary. For a particular ion type, this limit depends on its m/z ratio and ion mobility.

Chiral analysis of UV nonabsorbing compounds by capillary electrophoresis using macrocyclic antibiotics: 1. Separation of aspartic and glutamic acid enantiomers.

PubMed

Bednar, P; Aturki, Z; Stransky, Z; Fanali, S

2001-07-01

Glycopeptide antibiotics, namely vancomycin or teicoplanin, were evaluated in capillary electrophoresis for the analysis of UV nonabsorbing compounds such as aspartic and glutamic acid enantiomers. Electrophoretic runs were performed in laboratory-made polyacrylamide-coated capillaries using the partial filling-counter current method in order to avoid the presence on the detector path of the absorbing chiral selector. The background electrolyte consisted of an aqueous or aqueous-organic buffer in the pH range of 4.5-6.5 of sorbic acid/histidine and the appropriate concentration of chiral selector. Several experimental parameters such as antibiotic concentration and type, buffer pH, organic modifier, type and concentration of absorbing co-ion (for the indirect UV detection) were studied in order to find the optimum conditions for the chiral resolution of the two underivatized amino acids in their enantiomers. Among the two investigated chiral selectors, vancomycin resulted to be the most useful chiral selector allowing relatively high chiral resolution of the studied compounds even at low concentration. The optimized method (10 mM sorbic acid/histidine, pH 5, and 10 mM of vancomycin) was used for the analysis of real samples such as teeth dentine and beer.

Cerebral Microcirculation and Oxygen Tension in the Human Secondary Cortex

PubMed Central

Linninger, A. A.; Gould, I. G.; Marinnan, T.; Hsu, C.-Y.; Chojecki, M.; Alaraj, A.

2013-01-01

The three-dimensional spatial arrangement of the cortical microcirculatory system is critical for understanding oxygen exchange between blood vessels and brain cells. A three-dimensional computer model of a 3 × 3 × 3 mm3 subsection of the human secondary cortex was constructed to quantify oxygen advection in the microcirculation, tissue oxygen perfusion, and consumption in the human cortex. This computer model accounts for all arterial, capillary and venous blood vessels of the cerebral microvascular bed as well as brain tissue occupying the extravascular space. Microvessels were assembled with optimization algorithms emulating angiogenic growth; a realistic capillary bed was built with space filling procedures. The extravascular tissue was modeled as a porous medium supplied with oxygen by advection–diffusion to match normal metabolic oxygen demand. The resulting synthetic computer generated network matches prior measured morphometrics and fractal patterns of the cortical microvasculature. This morphologically accurate, physiologically consistent, multi-scale computer network of the cerebral microcirculation predicts the oxygen exchange of cortical blood vessels with the surrounding gray matter. Oxygen tension subject to blood pressure and flow conditions were computed and validated for the blood as well as brain tissue. Oxygen gradients along arterioles, capillaries and veins agreed with in vivo trends observed recently in imaging studies within experimental tolerances and uncertainty. PMID:23842693

Liquid-metal plasma-facing component research on the National Spherical Torus Experiment

NASA Astrophysics Data System (ADS)

Jaworski, M. A.; Khodak, A.; Kaita, R.

2013-12-01

Liquid metal plasma-facing components (PFCs) have been proposed as a means of solving several problems facing the creation of economically viable fusion power reactors. Liquid metals face critical issues in three key areas: free-surface stability, material migration and demonstration of integrated scenarios. To date, few demonstrations exist of this approach in a diverted tokamak and we here provide an overview of such work on the National Spherical Torus Experiment (NSTX). The liquid lithium divertor (LLD) was installed and operated for the 2010 run campaign using evaporated coatings as the filling method. Despite a nominal liquid level exceeding the capillary structure and peak current densities into the PFCs exceeding 100 kA m-2, no macroscopic ejection events were observed. The stability can be understood from a Rayleigh-Taylor instability analysis. Capillary restraint and thermal-hydraulic considerations lead to a proposed liquid-metal PFCs scheme of actively-supplied, capillary-restrained systems. Even with state-of-the-art cooling techniques, design studies indicate that the surface temperature with divertor-relevant heat fluxes will still reach temperatures above 700 °C. At this point, one would expect significant vapor production from a liquid leading to a continuously vapor-shielded regime. Such high-temperature liquid lithium PFCs may be possible on the basis of momentum-balance arguments.

Streptavidin-functionalized capillary immune microreactor for highly efficient chemiluminescent immunoassay.

PubMed

Yang, Zhanjun; Zong, Chen; Ju, Huangxian; Yan, Feng

2011-11-07

A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron microscopy. Compared to conventional packed tube or thin-layer cell immunoreactor, the proposed microreactor showed remarkable properties such as lower cost, simpler fabrication, better practicality and wider dynamic range for fast CL immunoassay with good reproducibility and stability. Using α-fetoprotein as model analyte, the highly efficient CL flow-through immunoassay system showed a linear range of 3 orders of magnitude from 0.5 to 200 ng mL(-1) and a low detection limit of 0.1 ng mL(-1). The capillary immune microreactor could make up the shortcoming of conventional CL immunoreactors and provided a promising alternative for highly efficient flow-injection immunoassay. Copyright © 2011 Elsevier B.V. All rights reserved.

End-faced waveguide mediated optical propulsion of microspheres and single cells in a microfluidic device.

PubMed

Lilge, Lothar; Shah, Duoaud; Charron, Luc

2013-07-07

Single cell transport in microfluidic devices is a topic of interest as their utility is becoming appreciated by cell and molecular biologist. Cell transport should minimize mechanical stress due to friction or pressure gradients. Optical forces have the advantage of applying their forces across the cell volume and not only at the cell membrane and are thus preferable. Optical pushing by scattering force is a suitable candidate so highly dependent on the photon irradiance field inside the propagation capillary which in turn is determined by the waveguide properties delivering the radiation pressure. Here we present a numerical approach to predict the optical scattering force, speed and trajectory of cells as a function of waveguide and propagation capillary geometry. Experimental verification of the simulation approach is demonstrated using polystyrene microspheres and leukemia cells. Effects of optical fibre to waveguide alignment, capillary wall angle and temperature on the dynamic viscosity on speed and position of the microspheres and cells inside the propagation capillary are demonstrated.

The Dynamics of Glomerular Ultrafiltration in the Rat

PubMed Central

Brenner, Barry M.; Troy, Julia L.; Daugharty, Terrance M.

1971-01-01

Using a unique strain of Wistar rats endowed with glomeruli situated directly on the renal cortical surface, we measured glomerular capillary pressures using servo-nulling micropipette transducer techniques. Pressures in 12 glomerular capillaries from 7 rats averaged 60 cm H2O, or approximately 50% of mean systemic arterial values. Wave form characteristics for these glomerular capillaries were found to be remarkably similar to those of the central aorta. From similarly direct estimates of hydrostatic pressures in proximal tubules, and colloid osmotic pressures in systemic and efferent arteriolar plasmas, the net driving force for ultrafiltration was calculated. The average value of 14 cm H2O is lower by some two-thirds than the majority of estimates reported previously based on indirect techniques. Single nephron GFR (glomerular filtration rate) was also measured in these rats, thereby permitting calculation of the glomerular capillary ultrafiltration coefficient. The average value of 0.044 nl sec−1 cm H2O−1 glomerulus−1 is at least fourfold greater than previous estimates derived from indirect observations. PMID:5097578

Gas Flow and Ion Transfer in Heated ESI Capillary Interfaces

NASA Astrophysics Data System (ADS)

Bernier, Laurent; Pinfold, Harry; Pauly, Matthias; Rauschenbach, Stephan; Reiss, Julius

2018-02-01

Transfer capillaries are the preferred means to transport ions, generated by electrospray ionization, from ambient conditions to vacuum. During the transfer of ions through the narrow, long tubes into vacuum, substantial losses are typical. However, recently it was demonstrated that these losses can be avoided altogether. To understand the experimental observation and provide a general model for the ion transport, here, we investigate the ion transport through capillaries by numerical simulation of interacting ions. The simulation encompasses all relevant factors, such as space charge, diffusion, gas flow, and heating. Special attention is paid to the influence of the gas flow on the transmission and especially the change imposed by heating. The gas flow is modeled by a one-dimensional gas dynamics description. A large number of ions are treated as point particles in this gas flow. This allows to investigate the influence of the capillary heating on the gas flow and by this on the ion transport. The results are compared with experimental findings. [Figure not available: see fulltext.

Inkjet Printing Based Droplet Generation for Integrated Online Digital Polymerase Chain Reaction.

PubMed

Zhang, Weifei; Li, Nan; Koga, Daisuke; Zhang, Yong; Zeng, Hulie; Nakajima, Hizuru; Lin, Jin-Ming; Uchiyama, Katsumi

2018-04-17

We report on the development of a novel and flexible online digital polymerase chain reaction (dPCR) system. The system was composed of three parts: an inkjet for generating the droplets, a coiled fused-silica capillary for thermal cycling, and a laser-induced fluorescence detector (LIFD) for positive droplet counting. Upon inkjet printing, monodisperse droplets were continuously generated in the oil phase and then introduced into the capillary in the form of a stable dispersion. The droplets containing one or zero molecules of target DNA passed through the helical capillary that was attached to a cylindrical thermal cycler for PCR amplification, resulting in the generation of fluorescence for the DNA-positive droplet. After 36 PCR cycles, the fluorescence signal intensity was detected by laser-induced fluorescence located at the downstream of the capillary, followed by a positive/negative counting. The present system was successfully applied to the absolute quantification of the HPV sequence in Caski cells with dynamic ranges spanning 4 orders of magnitude.

Control of evaporation by geometry in capillary structures. From confined pillar arrays in a gap radial gradient to phyllotaxy-inspired geometry.

PubMed

Chen, Chen; Duru, Paul; Joseph, Pierre; Geoffroy, Sandrine; Prat, Marc

2017-11-08

Evaporation is a key phenomenon in the natural environment and in many technological systems involving capillary structures. Understanding the evaporation front dynamics enables the evaporation rate from microfluidic devices and porous media to be finely controlled. Of particular interest is the ability to control the position of the front through suitable design of the capillary structure. Here, we show how to design model capillary structures in microfluidic devices so as to control the drying kinetics. This is achieved by acting on the spatial organization of the constrictions that influence the invasion of the structure by the gas phase. Two types of control are demonstrated. The first is intended to control the sequence of primary invasions through the pore space, while the second aims to control the secondary liquid structures: films, bridges, etc., that can form in the region of pore space invaded by the gas phase. It is shown how the latter can be obtained from phyllotaxy-inspired geometry. Our study thus opens up a route toward the control of the evaporation kinetics by means of tailored capillary structures.

In Situ Determination of Illegal Drugs in Oral Fluid by Portable Capillary Electrophoresis with Deep UV Excited Fluorescence Detection.

PubMed

Saar-Reismaa, Piret; Erme, Enn; Vaher, Merike; Kulp, Maria; Kaljurand, Mihkel; Mazina-Šinkar, Jekaterina

2018-05-15

The present study demonstrates the potential of a portable capillary electrophoresis (CE) instrument, coupled to deep UV fluorescence detector (FD) with a 230-255 nm excitation wavelength range, for the determination of the abuse of illegal drugs in oral fluids in situ. CE was introduced in this study due its exceptional power of separation and resolution, short analysis time, and ability for miniaturization for on-site assessment of different substances. The deep UV fluorescence detector was equipped with five interchangeable emission filters, in the emission wavelength range from 278 to 600 nm, and was successfully employed for determination of natively fluorescing illegal drugs, such as cocaine, cocaethylene, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxeamphetamine (MDA), 3,4-methylenedioxy- N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxy- N-methylamphetamine (PMMA), amphetamine (AMP), methamphetamine (METH), tetrahydrocannabinol (THC) and cannabidiol (CBD). The developed FD showed impressive sensitivity. The instrumental detection limit was 0.5 μg/L for MDMA. It also showed broad linearity, up to 50 mg/L for MDMA. The noise CV% was 1.1% for an empty capillary and 0.6% for a capillary filled with acetonitrile. The portable CE-FD with developed electrophoretic methodologies was successfully utilized for the determination of illegal abuse of drugs during "Weekend" 2016 and 2017 Music Festivals (Estonia). Moreover, CE-FD can be employed for detection of other natively fluorescing compounds in the proposed range (e.g., for different phenolic compounds, BTEX, naphthalene derivatives, and others), significantly widening the applicability of developed CE-FD instrument.

A visualization study on two-phase gravity drainage in porous media by using magnetic resonance imaging.

PubMed

Teng, Ying; Liu, Yu; Jiang, Lanlan; Song, Yongchen; Zhao, Jiafei; Zhang, Yi; Wang, Dayong

2016-09-01

Gravity drainage characteristics are important to improve our understanding of gas-liquid or liquid-liquid two-phase flow in porous media. Stable or unstable displacement fronts that controlled by the capillary force, viscous force, gravitational force, etc., are relevant features of immiscible two-phase flow. In this paper, three dimensionless parameters, namely, the gravity number, the capillary number and the Bond number, were used to describe the effect of the above mentioned forces on two-phase drainage features, including the displacement front and final displacing-phase saturation. A series of experiments on the downward displacement of a viscous fluid by a less viscous fluid in a vertical vessel that is filled with quartz beads are performed by using magnetic resonance imaging (MRI). The experimental results indicate that the wetting properties at both high and low capillary numbers exert remarkable control on the fluid displacement. When the contact angle is lower than 90°, i.e., the displaced phase is the wetting phase, the average velocity Vf of the interface of the two phases (displacement front velocity) is observably lower than when the displaced phase is the non-wetting phase (contact angle higher than 90°). The results show that a fingering phenomenon occurs when the gravity number G is less than the critical gravity number G'=Δμ/μg. Moreover, the higher Bond number results in higher final displacing-phase saturation, whereas the capillary number has an opposite effect. Copyright © 2016 Elsevier Inc. All rights reserved.

Drop formation, pinch-off dynamics and liquid transfer of simple and complex fluids

NASA Astrophysics Data System (ADS)

Dinic, Jelena; Sharma, Vivek

Liquid transfer and drop formation processes underlying jetting, spraying, coating, and printing - inkjet, screen, roller-coating, gravure, nanoimprint hot embossing, 3D - often involve formation of unstable columnar necks. Capillary-driven thinning of such necks and their pinchoff dynamics are determined by a complex interplay of inertial, viscous and capillary stresses for simple, Newtonian fluids. Micro-structural changes in response to extensional flow field that arises within the thinning neck give rise to additional viscoelastic stresses in complex, non- Newtonian fluids. Using FLOW-3D, we simulate flows realized in prototypical geometries (dripping and liquid bridge stretched between two parallel plates) used for studying pinch-off dynamics and influence of microstructure and viscoelasticity. In contrast with often-used 1D or 2D models, FLOW-3D allows a robust evaluation of the magnitude of the underlying stresses and extensional flow field (both uniformity and magnitude). We find that the simulated radius evolution profiles match the pinch-off dynamics that are experimentally-observed and theoretically-predicted for model Newtonian fluids and complex fluids.

Curvature capillary migration of microspheres.

PubMed

Sharifi-Mood, Nima; Liu, Iris B; Stebe, Kathleen J

2015-09-14

We address the question: how does capillarity propel microspheres along curvature gradients? For a particle on a fluid interface, there are two conditions that can apply at the three phase contact line: either the contact line adopts an equilibrium contact angle, or it can be pinned by kinetic trapping, e.g. at chemical heterogeneities, asperities, or other pinning sites on the particle surface. We formulate the curvature capillary energy for both scenarios for particles smaller than the capillary length and far from any pinning boundaries. The scale and range of the distortion made by the particle are set by the particle radius; we use singular perturbation methods to find the distortions and to rigorously evaluate the associated capillary energies. For particles with equilibrium contact angles, contrary to the literature, we find that the capillary energy is negligible, with the first contribution bounded to fourth order in the product of the particle radius and the deviatoric curvature of the host interface. For pinned contact lines, we find curvature capillary energies that are finite, with a functional form investigated previously by us for disks and microcylinders on curved interfaces. In experiments, we show microspheres migrate along deterministic trajectories toward regions of maximum deviatoric curvature with curvature capillary energies ranging from 6 × 10(3)-5 × 10(4)kBT. These data agree with the curvature capillary energy for the case of pinned contact lines. The underlying physics of this migration is a coupling of the interface deviatoric curvature with the quadrupolar mode of nanometric disturbances in the interface owing to the particle's contact line undulations. This work is an example of the major implications of nanometric roughness and contact line pinning for colloidal dynamics.

The effect of a microscale fracture on dynamic capillary pressure of two-phase flow in porous media

NASA Astrophysics Data System (ADS)

Tang, Mingming; Lu, Shuangfang; Zhan, Hongbin; Wenqjie, Guo; Ma, Huifang

2018-03-01

Dynamic capillary pressure (DCP) effects, which is vital for predicting multiphase flow behavior in porous media, refers to the injection rate dependence capillary pressure observed during non-equilibrium displacement experiments. However, a clear picture of the effects of microscale fractures on DCP remains elusive. This study quantified the effects of microscale fractures on DCP and simulated pore-scale force and saturation change in fractured porous media using the multiphase lattice Boltzmann method (LBM). Eighteen simulation cases were carried out to calculate DCP as a function of wetting phase saturation. The effects of viscosity ratio and fracture orientation, aperture and length on DCP and DCP coefficient τ were investigated, where τ refers to the ratio of the difference of DCP and static capillary pressure (SCP) over the rate of wetting-phase saturation change versus time. Significant differences in τ values were observed between unfractured and fractured porous media. The τ values of fractured porous media were 1.1  × 104 Pa ms to 5.68 × 105 Pa ms, which were one or two orders of magnitude lower than those of unfractured porous media with a value of 4 × 106 Pa. ms. A horizontal fracture had greater effects on DCP and τ than a vertical fracture, given the same fracture aperture and length. This study suggested that a microscale fracture might result in large magnitude changes in DCP for two-phase flow.

The Dynamics of Miscible Interfaces: Simulations

NASA Technical Reports Server (NTRS)

Meiburg, Eckart

2002-01-01

The goal of this experimental/computational investigation (joint with Prof Maxworthy at USC) has been to study the dynamics of miscible interfaces, both from a scientific and a practical point of view, and to prepare a related experiment to be flown on the International Space Station. In order to address these effects, we have focused experimental and computational investigations on miscible displacements in cylindrical capillary tubes, as well as in Hele-Shaw cells. Regarding the flow in a capillary tube, the question was addressed as to whether Korteweg stresses and/or divergence effects can potentially account for discrepancies observed between conventional Stokes flow simulations and experiments for miscible flows in capillary tubes. An estimate of the vorticity and streamfunction fields induced by the Kortewegs stresses was derived, which shows these stresses to result in the formation of a vortex ring structure near the tip of the concentration front. Through this mechanism the propagation velocity of the concentration front is reduced, in agreement with the experimental observations. Divergence effects, on the other hand, were seen to be very small, and they have a negligible influence on the tip velocity. As a result, it can be concluded that they are not responsible for the discrepancies between experiments and conventional Stokes simulations. A further part of our investigation focussed on the development of high-accuracy three-dimensional spectral element simulation techniques for miscible flows in capillary tubes, including the effects of variable density and viscosity. Towards this end, the conservation equations are treated in cylindrical coordinates.

Conceptual design of a pre-loaded liquid lithium divertor target for NSTX-U

DOE PAGES

Rindt, P.; Lopes Cardozo, N. J.; van Dommelen, J. A. W.; ...

2016-09-03

In this study, a conceptual design for a pre-filled liquid lithium divertor target for the National Spherical Torus Experiment Upgrade (NSTX-U) is presented. The design is aimed at facilitating experiments with high lithium flux from the plasma facing components (PFCs) in NSTX-U and investigating the potential of capillary based liquid lithium components. In the design, lithium is supplied from a reservoir in the PFC to the plasma facing surface via capillary action in a wicking structure. This working principle is also demonstrated experimentally. Next, a titanium zirconium molybdenum (TZM) prototype design is presented, required to withstand a steady state heatmore » flux peaking at 10 MW m –2 for 5 s and edge localized modes depositing (130 kJ in 2 ms at 10 Hz). The main challenge is to sufficiently reduce the thermal stresses. This is achieved by dividing the surface into brushes and filling the slots in between with liquid lithium. The principle of using this liquid “interlayer” allows for thermal expansion and simultaneously heat conduction, and could be used to significantly reduce the demands to solids in future PFCs. Lithium flow to the surface is analyzed using a novel analytical model, ideally suited for design purposes. Thermal stresses in the PFC are analyzed using the finite element method. As a result, the requirements are met, and thus a prototype will be manufactured for physical testing.« less

Self-interference fluorescence microscopy with three-phase detection for depth-resolved confocal epi-fluorescence imaging.

PubMed

Braaf, Boy; de Boer, Johannes F

2017-03-20

Three-dimensional confocal fluorescence imaging of in vivo tissues is challenging due to sample motion and limited imaging speeds. In this paper a novel method is therefore presented for scanning confocal epi-fluorescence microscopy with instantaneous depth-sensing based on self-interference fluorescence microscopy (SIFM). A tabletop epi-fluorescence SIFM setup was constructed with an annular phase plate in the emission path to create a spectral self-interference signal that is phase-dependent on the axial position of a fluorescent sample. A Mach-Zehnder interferometer based on a 3 × 3 fiber-coupler was developed for a sensitive phase analysis of the SIFM signal with three photon-counter detectors instead of a spectrometer. The Mach-Zehnder interferometer created three intensity signals that alternately oscillated as a function of the SIFM spectral phase and therefore encoded directly for the axial sample position. Controlled axial translation of fluorescent microsphere layers showed a linear dependence of the SIFM spectral phase with sample depth over axial image ranges of 500 µm and 80 µm (3.9 × Rayleigh range) for 4 × and 10 × microscope objectives respectively. In addition, SIFM was in good agreement with optical coherence tomography depth measurements on a sample with indocyanine green dye filled capillaries placed at multiple depths. High-resolution SIFM imaging applications are demonstrated for fluorescence angiography on a dye-filled capillary blood vessel phantom and for autofluorescence imaging on an ex vivo fly eye.

Relationship of echocardiographic indices to pulmonary capillary wedge pressures in healthy volunteers

NASA Technical Reports Server (NTRS)

Firstenberg, M. S.; Levine, B. D.; Garcia, M. J.; Greenberg, N. L.; Cardon, L.; Morehead, A. J.; Zuckerman, J.; Thomas, J. D.

2000-01-01

OBJECTIVES: We sought to determine the relationship between different echocardiographic indices and pulmonary capillary wedge pressures (PCWP) in normal volunteers. BACKGROUND: Indices based on tissue Doppler (TDE) and color M-mode (CMM) echocardiography have been proposed to reflect left (LV) ventricular filling pressures. These include the ratio of early diastolic transmitral velocity (E) to early myocardial velocity measured by TDE (E') and the ratio of E to the wave propagation velocity (Vp) measured from CMM images. These indices, however, have not been validated in normal individuals. METHODS: We studied seven volunteers during two phases of preload altering maneuvers, baseline, with two stages of lower body negative pressure, and repeat baseline with two stages of volume loading. The PCWP obtained from right heart catheterization was compared with diastolic indices using pulsed Doppler, TDE and CMM echocardiography. RESULTS: The PCWP ranged from 2.2 to 23.5 mm Hg. During preload alterations, significant changes in E and septal E' (both p < 0.05) but not lateral E' or Vp were observed. Furthermore, E, septal E' and E/Vp correlated with PCWP (all r > 0.80) but not combined E and TDE indices (both r < 0.15). Within individuals, a similar linear relationship was observed among E/Vp, E and septal E' (average r > 0.80). CONCLUSIONS: In subjects without heart disease, E, septal E' and E/Vp correlate with PCWP. Because the influence of ventricular relaxation is minimized, the ratio E/Vp may be the best overall index of LV filling pressures.

Conceptual design of a pre-loaded liquid lithium divertor target for NSTX-U

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rindt, P.; Lopes Cardozo, N. J.; van Dommelen, J. A. W.

In this study, a conceptual design for a pre-filled liquid lithium divertor target for the National Spherical Torus Experiment Upgrade (NSTX-U) is presented. The design is aimed at facilitating experiments with high lithium flux from the plasma facing components (PFCs) in NSTX-U and investigating the potential of capillary based liquid lithium components. In the design, lithium is supplied from a reservoir in the PFC to the plasma facing surface via capillary action in a wicking structure. This working principle is also demonstrated experimentally. Next, a titanium zirconium molybdenum (TZM) prototype design is presented, required to withstand a steady state heatmore » flux peaking at 10 MW m –2 for 5 s and edge localized modes depositing (130 kJ in 2 ms at 10 Hz). The main challenge is to sufficiently reduce the thermal stresses. This is achieved by dividing the surface into brushes and filling the slots in between with liquid lithium. The principle of using this liquid “interlayer” allows for thermal expansion and simultaneously heat conduction, and could be used to significantly reduce the demands to solids in future PFCs. Lithium flow to the surface is analyzed using a novel analytical model, ideally suited for design purposes. Thermal stresses in the PFC are analyzed using the finite element method. As a result, the requirements are met, and thus a prototype will be manufactured for physical testing.« less

CO2 Capillary-Trapping Processes in Deep Saline Aquifers

NASA Astrophysics Data System (ADS)

Gershenzon, Naum I.; Soltanian, Mohamadreza; Ritzi, Robert W., Jr.; Dominic, David F.

2014-05-01

The idea of reducing the Earth's greenhouse effect by sequestration of CO2 into the Earth's crust has been discussed and evaluated for more than two decades. Deep saline aquifers are the primary candidate formations for realization of this idea. Evaluation of reservoir capacity and the risk of CO2 leakage require a detailed modeling of the migration and distribution of CO2 in the subsurface structure. There is a finite risk that structural (or hydrodynamic) trapping by caprock may be compromised (e.g. by improperly abandoned wells, stratigraphic discontinuities, faults, etc.). Therefore, other trapping mechanisms (capillary trapping, dissolution, and mineralization) must be considered. Capillary trapping may be very important in providing a "secondary-seal", and is the focus of our investigation. The physical mechanism of CO2 trapping in porous media by capillary trapping incorporates three related processes, i.e. residual trapping, trapping due to hysteresis of the relative permeability, and trapping due to hysteresis of the capillary pressure. Additionally CO2 may be trapped in heterogeneous media due to difference in capillary pressure entry points for different materials. The amount of CO2 trapped by these processes is a complicated nonlinear function of the spatial distribution of permeability, permeability anisotropy, capillary pressure, relative permeability of brine and CO2, permeability hysteresis and residual gas saturation (as well as the rate, total amount and placement of injected CO2). Geological heterogeneities essentially affect the dynamics of a CO2 plume in subsurface environments. Recent studies have led to new conceptual and quantitative models for sedimentary architecture in fluvial deposits over a range of scales that are relevant to the performance of some deep saline reservoirs [1, 2]. We investigated how the dynamics of a CO2 plume, during and after injection, is influenced by the hierarchical and multi-scale stratal architecture in such reservoirs. The results strongly suggest that representing these small scales features, and representing how they are organized within a hierarchy of larger-scale features, is critical to understanding capillary trapping processes. References [1] Bridge, J.S. (2006), Fluvial facies models: Recent developments, in Facies Models Revisited, SEPM Spec. Publ., 84, edited by H. W. Posamentier and R. G. Walker, pp. 85-170, Soc. for Sediment. Geol. (SEPM), Tulsa, Okla [2] Ramanathan, R., A. Guin, R.W. Ritzi, D.F. Dominic, V.L. Freedman, T.D. Scheibe, and I.A. Lunt (2010), Simulating the heterogeneity in channel belt deposits: Part 1. A geometric-based methodology and code, Water Resources Research, v. 46, W04515.

The hummingbird's tongue: a self-assembling capillary syphon

PubMed Central

Kim, Wonjung; Peaudecerf, François; Baldwin, Maude W.; Bush, John W. M.

2012-01-01

We present the results of a combined experimental and theoretical investigation of the dynamics of drinking in ruby-throated hummingbirds. In vivo observations reveal elastocapillary deformation of the hummingbird's tongue and capillary suction along its length. By developing a theoretical model for the hummingbird's drinking process, we investigate how the elastocapillarity affects the energy intake rate of the bird and how its open tongue geometry reduces resistance to nectar uptake. We note that the tongue flexibility is beneficial for accessing, transporting and unloading the nectar. We demonstrate that the hummingbird can attain the fastest nectar uptake when its tongue is roughly semicircular. Finally, we assess the relative importance of capillary suction and a recently proposed fluid trapping mechanism, and conclude that the former is important in many natural settings. PMID:23075839

Dynamic Contrast-Enhanced Magnetic Resonance Imaging Reveals Stress-Induced Angiogenesis in MCF7 Human Breast Tumors

NASA Astrophysics Data System (ADS)

Furman-Haran, Edna; Margalit, Raanan; Grobgeld, Dov; Degani, Hadassa

1996-06-01

The mechanism of contrast enhancement of tumors using magnetic resonance imaging was investigated in MCF7 human breast cancer implanted in nude mice. Dynamic contrast-enhanced images recorded at high spatial resolution were analyzed by an image analysis method based on a physiological model, which included the blood circulation, the tumor, the remaining tissues, and clearance via the kidneys. This analysis enabled us to map in rapidly enhancing regions within the tumor, the capillary permeability factor (capillary permeability times surface area per voxel volume) and the fraction of leakage space. Correlation of these maps with T2-weighted spin echo images, with histopathology, and with immunohistochemical staining of endothelial cells demonstrated the presence of dense permeable microcapillaries in the tumor periphery and in intratumoral regions that surrounded necrotic loci. The high leakage from the intratumoral permeable capillaries indicated an induction of a specific angiogenic process associated with stress conditions that cause necrosis. This induction was augmented in tumors responding to tamoxifen treatment. Determination of the distribution and extent of this stress-induced angiogenic activity by contrast-enhanced MRI might be of diagnostic and of prognostic value.

Dynamic wetting failure in surfactant solutions

NASA Astrophysics Data System (ADS)

Liu, Chen-Yu; Vandre, Eric; Carvalho, Marcio; Kumar, Satish

2015-11-01

The influence of insoluble surfactants on dynamic wetting failure during displacement of Newtonian fluids in a rectangular channel is studied in this work. A hydrodynamic model for steady Stokes flows of dilute surfactant solutions is developed and evaluated using three approaches: (i) a one-dimensional (1D) lubrication-type approach, (ii) a novel hybrid of a 1D description of the receding phase and a 2D description of the advancing phase, and (iii) an asymptotic theory of Cox. Steady-state solution families in the form of macroscopic contact angles as a function of the capillary number are determined and limit points are identified. When air is the receding fluid, Marangoni stresses are found to increase the receding-phase pressure gradients near the contact line by thinning the air film without significantly changing the capillary-pressure gradients there. As consequence, the limit points shift to lower capillary numbers and the onset of wetting failure is promoted. The model predictions are then used to interpret decades-old experimental observations concerning the influence of surfactants on air entrainment. The hybrid modeling approach developed here can readily be extended to more complicated geometries where a thin air layer is present near a contact line.

The Micromechanics of the Moving Contact Line

NASA Technical Reports Server (NTRS)

Lichter, Seth

1999-01-01

A transient moving contact line is investigated experimentally. The dynamic interface shape between 20 and 800 microns from the contact line is compared with theory. A novel experiment is devised, in which the contact line is set into motion by electrically altering the solid-liquid surface tension gamma(sub SL). The contact line motion simulates that of spontaneous wetting along a vertical plate with a maximum capillary number Ca approx. = 4 x 10(exp -2). The images of the dynamic meniscus are analyzed as a funtion of Ca. For comparison, the steady-state hydrodynamic equation based on the creeping flow model in a wedge geometry and the three-region uniform perturbation expansion of Cox (1986) is adopted. The interface shape is well depicted by the uniform solutions for Ca <= 10(exp -3). However, for Ca > 10(exp -3), the uniform solution over-predicts the viscous bending. This over-prediction can be accounted for by modifying the slip coefficient within the intermediate solution. With this correction, the measured interface shape is seen to match the theoretical prediction for all capillary numbers. The amount of slip needed to fit the measurements does not scale with the capillary number.

Easy-to-use microfluidic chip for long-term 3D-cell cultures

NASA Astrophysics Data System (ADS)

Bunge, Frank; van den Driesche, Sander; Vellekoop, Michael J.

2017-05-01

We present a microfluidic chip for an easy setup of a 3D-culture of mammalian cells. The chip contains feeding structures and gas supply for long-term cultivation of mammalian cells. The device is fabricated out of hard materials like silicon and glass that are all highly biocompatible. The chip uses the concept of surficial phaseguides that allows the partial filling of a microfluidic chip with liquids based on hydrophobic and hydrophilic surfaces. Here, a suspension of mammalian cells and melted agarose is filled into the chip and is pulled by the capillary pressure on the hydrophilic areas but not on the hydrophobic phaseguides. Consequently, only a part of the chip is filled with the agarose which gels by cooling a form the 3D-cell culture. The unfilled areas are used as supply structures for nutrition and gases. So the supply is based on diffusion and the supply of nutrition and gases is controlled independently. We cultured HaCaT-cells over 24 hours in our device and achieve a good viability.

Spontaneous imbibition in fractal tortuous micro-nano pores considering dynamic contact angle and slip effect: phase portrait analysis and analytical solutions.

PubMed

Li, Caoxiong; Shen, Yinghao; Ge, Hongkui; Zhang, Yanjun; Liu, Tao

2018-03-02

Shales have abundant micro-nano pores. Meanwhile, a considerable amount of fracturing liquid is imbibed spontaneously in the hydraulic fracturing process. The spontaneous imbibition in tortuous micro-nano pores is special to shale, and dynamic contact angle and slippage are two important characteristics. In this work, we mainly investigate spontaneous imbibition considering dynamic contact angle and slip effect in fractal tortuous capillaries. We introduce phase portrait analysis to analyse the dynamic state and stability of imbibition. Moreover, analytical solutions to the imbibition equation are derived under special situations, and the solutions are verified by published data. Finally, we discuss the influences of slip length, dynamic contact angle and gravity on spontaneous imbibition. The analysis shows that phase portrait is an ideal tool for analysing spontaneous imbibition because it can evaluate the process without solving the complex governing ordinary differential equations. Moreover, dynamic contact angle and slip effect play an important role in fluid imbibition in fractal tortuous capillaries. Neglecting slip effect in micro-nano pores apparently underestimates imbibition capability, and ignoring variations in contact angle causes inaccuracy in predicting imbibition speed at the initial stage of the process. Finally, gravity is one of the factors that control the stabilisation of the imbibition process.

MicroCT angiography detects vascular formation and regression in skin wound healing

PubMed Central

Urao, Norifumi; Okonkwo, Uzoagu A.; Fang, Milie M.; Zhuang, Zhen W.; Koh, Timothy J.; DiPietro, Luisa A.

2016-01-01

Properly regulated angiogenesis and arteriogenesis are essential for effective wound healing. Tissue injury induces robust new vessel formation and subsequent vessel maturation, which involves vessel regression and remodeling. Although formation of functional vasculature is essential for healing, alterations in vascular structure over the time course of skin wound healing are not well understood. Here, using high-resolution ex vivo X-ray micro-computed tomography (microCT), we describe the vascular network during healing of skin excisional wounds with highly detailed three-dimensional (3D) reconstructed images and associated quantitative analysis. We found that relative vessel volume, surface area and branching number are significantly decreased in wounds from day 7 to day 14 and 21. Segmentation and skeletonization analysis of selected branches from high-resolution images as small as 2.5 μm voxel size show that branching orders are decreased in the wound vessels during healing. In histological analysis, we found that the contrast agent fills mainly arterioles, but not small capillaries nor large veins. In summary, high-resolution microCT revealed dynamic alterations of vessel structures during wound healing. This technique may be useful as a key tool in the study of the formation and regression of wound vessels. PMID:27009591

Molecular dynamics simulation and NMR investigation of the association of the β-blockers atenolol and propranolol with a chiral molecular micelle

NASA Astrophysics Data System (ADS)

Morris, Kevin F.; Billiot, Eugene J.; Billiot, Fereshteh H.; Hoffman, Charlene B.; Gladis, Ashley A.; Lipkowitz, Kenny B.; Southerland, William M.; Fang, Yayin

2015-08-01

Molecular dynamics simulations and NMR spectroscopy were used to compare the binding of two β-blocker drugs to the chiral molecular micelle poly-(sodium undecyl-(L)-leucine-valine). The molecular micelle is used as a chiral selector in capillary electrophoresis. This study is part of a larger effort to understand the mechanism of chiral recognition in capillary electrophoresis by characterizing the molecular micelle binding of chiral compounds with different geometries and charges. Propranolol and atenolol were chosen because their structures are similar, but their chiral interactions with the molecular micelle are different. Molecular dynamics simulations showed both propranolol enantiomers inserted their aromatic rings into the molecular micelle core and that (S)-propranolol associated more strongly with the molecular micelle than (R)-propranolol. This difference was attributed to stronger molecular micelle hydrogen bonding interactions experienced by (S)-propranolol. Atenolol enantiomers were found to bind near the molecular micelle surface and to have similar molecular micelle binding free energies.

Surface Deformation by Thermo-capillary Convection -Sounding Rocket COMPERE Experiment SOURCE

NASA Astrophysics Data System (ADS)

Fuhrmann, Eckart; Dreyer, Michael E.

The sounding rocket COMPERE experiment SOURCE was successfully flown on MASER 11, launched in Kiruna (ESRANGE), May 15th, 2008. SOURCE has been intended to partly ful-fill the scientific objectives of the European Space Agency (ESA) Microgravity Applications Program (MAP) project AO-2004-111 (Convective boiling and condensation). Three parties of principle investigators have been involved to design the experiment set-up: ZARM for thermo-capillary flows, IMFT (Toulouse, France) for boiling studies, EADS Astrium (Bremen, Ger-many) for depressurization. The scientific aims are to study the effect of wall heat flux on the contact line of the free liquid surface and to obtain a correlation for a convective heat transfer coefficient. The experiment has been conducted along a predefined time line. A preheating sequence at ground was the first operation to achieve a well defined temperature evolution within the test cell and its environment inside the rocket. Nearly one minute after launch, the pressurized test cell was filled with the test liquid HFE-7000 until a certain fill level was reached. Then the free surface could be observed for 120 s without distortion. Afterwards, the first depressurization was started to induce subcooled boiling, the second one to start saturated boiling. The data from the flight consists of video images and temperature measurements in the liquid, the solid, and the gaseous phase. Data analysis provides the surface shape versus time and the corresponding apparent contact angle. Computational analysis provides information for the determination of the heat transfer coefficient in a compensated gravity environment where a flow is caused by the temperature difference between the hot wall and the cold liquid. Correlations for the effective contact angle and the heat transfer coefficient shall be delivered as a function of the relevant dimensionsless parameters. The data will be used for benchmarking of commercial CFD codes and the tank design (using these codes) of future cryogenic upper stages.

The Relationship Between Microscopic Grain Surface Structure and the Dynamic Capillary-Driven Advance of Water Films over Individual Dry Natural Sand Grains

NASA Astrophysics Data System (ADS)

Kibbey, T. C. G.; Adegbule, A.; Yan, S.

2017-12-01

The movement of nonvolatile solutes in unsaturated porous media at low water contents depends on transport in surface-associated water films. The focus of the work described here was on studying solute movement in water films advancing by capillary forces over initially-dry grain surfaces, to understand how microscopic surface roughness features influence the initial velocity of water film advance. For this work, water containing a non-adsorbing conservative tracer was used to track the movement of advancing water films. A stainless steel capillary tube connected to an external reservoir a fixed distance below the grain surface was used to transmit solution to the grain surface under negative pressure (positive capillary pressure), consistent with conditions that might be expected in the unsaturated zone. The small internal diameter of the capillary prevents solution from draining out of the capillary back into the reservoir. When the capillary is contacted with a grain surface, capillary forces that result from contact between the fluid and the rough grain surface cause water films to wick across the grain surface. Multiple experiments were conducted on the same grain, rotating the grain and varying the capillary contact point around the circumference of the grain. Imaging was conducted at fixed intervals using an automated Extended Depth of Field (EDF) imaging system, and images were analyzed to determine initial velocity. Grain surfaces were then characterized through scanning electron microscope (SEM) imaging, using a hybrid stereoscopic reconstruction method designed to extract maximum detail in creating elevation maps of geologic surfaces from tilted pairs of SEM images. The resulting elevation maps were used to relate surface roughness profiles around the grain with initial velocities. Results suggest that velocity varies significant with contact point around an individual grain, and correlates quantitatively with the local grain surface structure. Preliminary simulation results will also be discussed.

An air-pressure-free elastomeric valve for integrated nucleic acid analysis by capillary electrophoresis

NASA Astrophysics Data System (ADS)

Jung, Wooseok; Barrett, Matthew; Brooks, Carla; Rivera, Andrew; Birdsell, Dawn N.; Wagner, David M.; Zenhausern, Frederic

2015-12-01

We present a new elastomeric valve for integrated nucleic acid analysis by capillary electrophoresis. The valve functions include metering to capture a designated volume of biological sample into a polymerase chain reaction (PCR) chamber, sealing to preserve the sample during PCR cycling, and transfer of the PCR-products and on-chip formamide post-processing for the analysis of DNA fragments by capillary gel electrophoresis. This new valve differs from prior art polydimethylsiloxane (PDMS) valves in that the valve is not actuated externally by air-pressure or vacuum so that it simplifies a DNA analysis system by eliminating the need for an air-pressure or vacuum source, and off-cartridge solenoid valves, control circuit boards and software. Instead, the new valve is actuated by a thermal cycling peltier assembly integrated within the hardware instrument that tightly comes in contact with a microfluidic cartridge for thermal activation during PCR, so that it spontaneously closes the valve without an additional actuator system. The valve has bumps in the designated locations so that it has a self-alignment that does not require precise alignment of a valve actuator. Moreover, the thickness of the new valve is around 600 μm with an additional bump height of 400 μm so that it is easy to handle and very feasible to fabricate by injection molding compared to other PDMS valves whose thicknesses are around 30-100 μm. The new valve provided over 95% of metering performance in filling the fixed volume of the PCR chamber, preserved over 97% of the sample volume during PCR, and showed very comparable capillary electrophoresis peak heights to the benchtop assay tube controls with very consistent transfer volume of the PCR-product and on-chip formamide. The new valve can perform a core function for integrated nucleic acid analysis by capillary electrophoresis.

QENS investigation of filled rubbers

NASA Astrophysics Data System (ADS)

Triolo, A.; Lo Celso, F.; Negroni, F.; Arrighi, V.; Qian, H.; Lechner, R. E.; Desmedt, A.; Pieper, J.; Frick, B.; Triolo, R.

The polymer segmental dynamics is investigated in a series of silica-filled rubbers. The presence of inert fillers in polymers greatly affects the mechanical and physical performance of the final materials. For example, silica has been proposed as a reinforcing agent of elastomers in tire production. Results from quasielastic neutron scattering and Dynamic Mechanical Thermal Analysis (DMTA) measurements are presented on styrene-ran-butadiene rubber filled with silica. A clear indication is obtained of the existence of a bimodal dynamics, which can be rationalized in terms of the relaxation of bulk rubber and the much slower relaxation of the rubber adsorbed on the filler surface.

Extreme degree of ionization in homogenous micro-capillary plasma columns heated by ultrafast current pulses.

PubMed

Avaria, G; Grisham, M; Li, J; Tomasel, F G; Shlyaptsev, V N; Busquet, M; Woolston, M; Rocca, J J

2015-03-06

Homogeneous plasma columns with ionization levels typical of megaampere discharges are created by rapidly heating gas-filled 520-μm-diameter channels with nanosecond rise time current pulses of 40 kA. Current densities of up to 0.3  GA cm^{-2} greatly increase Joule heating with respect to conventional capillary discharge Z pinches, reaching unprecedented degrees of ionization for a high-Z plasma column heated by a current pulse of remarkably low amplitude. Dense xenon plasmas are ionized to Xe^{28+}, while xenon impurities in hydrogen discharges reach Xe^{30+}. The unique characteristics of these hot, ∼300:1 length-to-diameter aspect ratio plasmas allow the observation of unexpected spectroscopic phenomena. Axial spectra show the unusual dominance of the intercombination line over the resonance line of He-like Al by nearly an order of magnitude, caused by differences in opacities in the axial and radial directions. These plasma columns could enable the development of sub-10-nm x-ray lasers.

Counting polymers moving through a single ion channel

NASA Astrophysics Data System (ADS)

Bezrukov, Sergey M.; Vodyanoy, Igor; Parsegian, V. Adrian

1994-07-01

THE change in conductance of a small electrolyte-filled capillary owing to the passage of sub-micrometre-sized particles has long been used for particle counting and sizing. A commercial device for such measurements, the Coulter counter, is able to detect particles of sizes down to several tenths of a micrometre1-3. Nuclepore technology (in which pores are etched particle tracks) has extended the lower limit of size detection to 60-nm particles by using a capillary of diameter 0.45 μm (ref. 4). Here we show that natural channel-forming peptides incorporated into a bilayer lipid membrane can be used to detect the passage of single molecules with gyration radii as small as 5-15 Å. From our experiments with alamethicin pores we infer both the average number and the diffusion coefficients of poly(ethylene glycol) molecules in the pore. Our approach provides a means of observing the statistics and mechanics of flexible polymers moving within the confines of precisely defined single-molecule structures.

Comparison of CO2 trapping in highly heterogeneous reservoirs with Brooks-Corey and van Genuchten capillary pressure curves

NASA Astrophysics Data System (ADS)

Gershenzon, Naum; Soltanian, Mohamadreza; Ritzi, Robert, Jr.; Dominic, David

2015-04-01

Geological heterogeneities essentially affect the dynamics of a CO2 plume in subsurface environments. Recent studies have led to new conceptual and quantitative models for sedimentary architecture in fluvial deposits over a range of scales that are relevant to the performance of some deep saline reservoirs [1, 2]. Previously we showed how the dynamics of a CO2 plume, during and after injection, is influenced by the hierarchical and multi-scale stratal architecture in such reservoirs [3]. The results strongly suggest that representing these small scales (few cm in vertical direction and few meters in horizontal direction) features and representing how they are organized within a hierarchy of larger-scale features, is critical to understanding capillary trapping processes. The results also demonstrated the importance of using separate capillary pressure and relative permeability relationships for different textural facies types. Here we present the result of simulation of CO2 trapping in deep saline aquifers using two different conventional approaches, i.e. Brooks-Corey and van Genuchten, to capillary pressure. We showed that capillary trapping as well as dissolution rates are very different for the Brooks-Corey and van Genuchten approaches if reservoir consists from various species with different capillary pressure and relative permeability curves. We also found a dramatic difference in simulation time; using the van Genuchten approach improves convergence and thus reduces calculation time by one-two orders of magnitude. [1] Bridge, J.S. (2006), Fluvial facies models: Recent developments, in Facies Models Revisited, SEPM Spec. Publ., 84, edited by H. W. Posamentier and R. G. Walker, pp. 85-170, Soc. for Sediment. Geol. (SEPM), Tulsa, Okla [2] Ramanathan, R., A. Guin, R.W. Ritzi, D.F. Dominic, V.L. Freedman, T.D. Scheibe, and I.A. Lunt (2010), Simulating the heterogeneity in channel belt deposits: Part 1. A geometric-based methodology and code, Water Resources Research, v. 46, W04515. [3] Gershenzon N.I., M. Soltanian, R.W. Ritzi Jr., and D.F. Dominic (2014) Influence of small scale heterogeneity on CO2 trapping processes in deep saline aquifers, Energy Procedia, 59, 166 - 173.

Studies related to ocean dynamics. Task 3.2: Aircraft Field Test Program to investigate the ability of remote sensing methods to measure current/wind-wave interactions

NASA Technical Reports Server (NTRS)

Huang, N. E.; Flood, W. A.; Brown, G. S.

1975-01-01

The feasibility of remote sensing of current flows in the ocean and the remote sensing of ocean currents by backscattering cross section techniques was studied. It was established that for capillary waves, small scale currents could be accurately measured through observation of wave kinematics. Drastic modifications of waves by changing currents were noted. The development of new methods for the measurement of capillary waves are discussed. Improvement methods to resolve data processing problems are suggested.

The dynamics and stability of lubricating oil films during droplet transport by electrowetting in microfluidic devices.

PubMed

Kleinert, Jairus; Srinivasan, Vijay; Rival, Arnaud; Delattre, Cyril; Velev, Orlin D; Pamula, Vamsee K

2015-05-01

The operation of digital microfluidic devices with water droplets manipulated by electrowetting is critically dependent on the static and dynamic stability and lubrication properties of the oil films that separate the droplets from the solid surfaces. The factors determining the stability of the films and preventing surface fouling in such systems are not yet thoroughly understood and were experimentally investigated in this study. The experiments were performed using a standard digital microfluidic cartridge in which water droplets enclosed in a thin, oil-filled gap were transported over an array of electrodes. Stable, continuous oil films separated the droplets from the surfaces when the droplets were stationary. During droplet transport, capillary waves formed in the films on the electrode surfaces as the oil menisci receded. The waves evolved into dome-shaped oil lenses. Droplet deformation and oil displacement caused the films at the surface opposite the electrode array to transform into dimples of oil trapped over the centers of the droplets. Lower actuation voltages were associated with slower film thinning and formation of fewer, but larger, oil lenses. Lower ac frequencies induced oscillations in the droplets that caused the films to rupture. Films were also destabilized by addition of surfactants to the oil or droplet phases. Such a comprehensive understanding of the oil film behavior will enable more robust electrowetting-actuated lab-on-a-chip devices through prevention of loss of species from droplets and contamination of surfaces at points where films may break.

The dynamics and stability of lubricating oil films during droplet transport by electrowetting in microfluidic devices

PubMed Central

Kleinert, Jairus; Srinivasan, Vijay; Rival, Arnaud; Delattre, Cyril; Velev, Orlin D.; Pamula, Vamsee K.

2015-01-01

The operation of digital microfluidic devices with water droplets manipulated by electrowetting is critically dependent on the static and dynamic stability and lubrication properties of the oil films that separate the droplets from the solid surfaces. The factors determining the stability of the films and preventing surface fouling in such systems are not yet thoroughly understood and were experimentally investigated in this study. The experiments were performed using a standard digital microfluidic cartridge in which water droplets enclosed in a thin, oil-filled gap were transported over an array of electrodes. Stable, continuous oil films separated the droplets from the surfaces when the droplets were stationary. During droplet transport, capillary waves formed in the films on the electrode surfaces as the oil menisci receded. The waves evolved into dome-shaped oil lenses. Droplet deformation and oil displacement caused the films at the surface opposite the electrode array to transform into dimples of oil trapped over the centers of the droplets. Lower actuation voltages were associated with slower film thinning and formation of fewer, but larger, oil lenses. Lower ac frequencies induced oscillations in the droplets that caused the films to rupture. Films were also destabilized by addition of surfactants to the oil or droplet phases. Such a comprehensive understanding of the oil film behavior will enable more robust electrowetting-actuated lab-on-a-chip devices through prevention of loss of species from droplets and contamination of surfaces at points where films may break. PMID:26045729

Development of an Uncooled Photomechanic Infrared Sensor Based on the IR Organ of the Pyrophilous Jewel Beetle Melanophila Acuminata

DTIC Science & Technology

2007-01-04

been found to be a suitable means for depositing thin films of chitosan onto the surface of a silicon wafer. A small amount of liquid solution is...response of the system to changes of the meniscus position in the capillary. This was done by gradually filling the working volume with an ionic liquid ...from the wetting of the electrodes by the ionic liquid . From this data we have calculated a signal to noise ratio of 100 for the read out mechanism

Retinovascular physiology and pathophysiology: new experimental approach/new insights

PubMed Central

Puro, Donald G.

2012-01-01

An important challenge in visual neuroscience is understand the physiology and pathophysiology of the intra-retinal vasculature, whose function is required for ophthalmoception by humans and most other mammals. In the quest to learn more about this highly specialized portion of the circulatory system, a newly developed method for isolating vast microvascular complexes from the rodent retina has opened the way for using techniques such as patch-clamping, fluorescence imaging and time-lapse photography to elucidate the functional organization of a capillary network and its pre-capillary arteriole. For example, the ability to obtain dual perforated-patch recordings from well-defined sites within an isolated microvascular complex permitted the first characterization of the electrotonic architecture of a capillary/arteriole unit. This analysis revealed that this operational unit is not simply a homogenous synctium, but has a complex functional organization that is dynamically modulated by extracellular signals such as angiotensin II. Another recent discovery is that a capillary and its pre-capillary arteriole have distinct physiological differences; capillaries have an abundance of ATP-sensitive potassium (KATP) channels and a dearth of voltage-dependent calcium channels (VDCCs) while the converse is true for arterioles. In addition, voltage transmission between abluminal cells and the endothelium is more efficient in the capillaries. Thus, the capillary network is well-equipped to generate and transmit voltages, and the pre-capillary arteriole is well-adapted to transduce a capillary-generated voltage into a change in abluminal cell calcium and thereby, a vasomotor response. Use of microvessels isolated from the diabetic retina has led to new insights concerning retinal vascular pathophysiology. For example, soon after the onset of diabetes, the efficacy of voltage transmission through the endothelium is diminished; arteriolar VDCCs is inhibited, and there is increased vulnerability to purinergic vasotoxicity, which is a newly identified pathobiological mechanism. Other recent studies reveal that KATP channels not only have an essential physiological role in generating vasomotor responses, but their activation substantially boosts the lethality of hypoxia. Thus, the pathophysiology of the retinal microvasculature is closely linked with its physiology. PMID:22333041

Effect of water table dynamics on land surface hydrologic memory

NASA Astrophysics Data System (ADS)

Lo, Min-Hui; Famiglietti, James S.

2010-11-01

The representation of groundwater dynamics in land surface models has received considerable attention in recent years. Most studies have found that soil moisture increases after adding a groundwater component because of the additional supply of water to the root zone. However, the effect of groundwater on land surface hydrologic memory (persistence) has not been explored thoroughly. In this study we investigate the effect of water table dynamics on National Center for Atmospheric Research Community Land Model hydrologic simulations in terms of land surface hydrologic memory. Unlike soil water or evapotranspiration, results show that land surface hydrologic memory does not always increase after adding a groundwater component. In regions where the water table level is intermediate, land surface hydrologic memory can even decrease, which occurs when soil moisture and capillary rise from groundwater are not in phase with each other. Further, we explore the hypothesis that in addition to atmospheric forcing, groundwater variations may also play an important role in affecting land surface hydrologic memory. Analyses show that feedbacks of groundwater on land surface hydrologic memory can be positive, negative, or neutral, depending on water table dynamics. In regions where the water table is shallow, the damping process of soil moisture variations by groundwater is not significant, and soil moisture variations are mostly controlled by random noise from atmospheric forcing. In contrast, in regions where the water table is very deep, capillary fluxes from groundwater are small, having limited potential to affect soil moisture variations. Therefore, a positive feedback of groundwater to land surface hydrologic memory is observed in a transition zone between deep and shallow water tables, where capillary fluxes act as a buffer by reducing high-frequency soil moisture variations resulting in longer land surface hydrologic memory.

Smartphone Cortex Controlled Real-Time Image Processing and Reprocessing for Concentration Independent LED Induced Fluorescence Detection in Capillary Electrophoresis.

PubMed

Szarka, Mate; Guttman, Andras

2017-10-17

We present the application of a smartphone anatomy based technology in the field of liquid phase bioseparations, particularly in capillary electrophoresis. A simple capillary electrophoresis system was built with LED induced fluorescence detection and a credit card sized minicomputer to prove the concept of real time fluorescent imaging (zone adjustable time-lapse fluorescence image processor) and separation controller. The system was evaluated by analyzing under- and overloaded aminopyrenetrisulfonate (APTS)-labeled oligosaccharide samples. The open source software based image processing tool allowed undistorted signal modulation (reprocessing) if the signal was inappropriate for the actual detection system settings (too low or too high). The novel smart detection tool for fluorescently labeled biomolecules greatly expands dynamic range and enables retrospective correction for injections with unsuitable signal levels without the necessity to repeat the analysis.

Disposable pen-shaped capillary gel electrophoresis cartridge for fluorescence detection of bio-molecules

NASA Astrophysics Data System (ADS)

Amirkhanian, Varoujan; Tsai, Shou-Kuan

2014-03-01

We introduce a novel and cost-effective capillary gel electrophoresis (CGE) system utilizing disposable pen-shaped gelcartridges for highly efficient, high speed, high throughput fluorescence detection of bio-molecules. The CGE system has been integrated with dual excitation and emission optical-fibers with micro-ball end design for fluorescence detection of bio-molecules separated and detected in a disposable pen-shaped capillary gel electrophoresis cartridge. The high-performance capillary gel electrophoresis (CGE) analyzer has been optimized for glycoprotein analysis type applications. Using commercially available labeling agent such as ANTS (8-aminonapthalene-1,3,6- trisulfonate) as an indicator, the capillary gel electrophoresis-based glycan analyzer provides high detection sensitivity and high resolving power in 2-5 minutes of separations. The system can hold total of 96 samples, which can be automatically analyzed within 4-5 hours. This affordable fiber optic based fluorescence detection system provides fast run times (4 minutes vs. 20 minutes with other CE systems), provides improved peak resolution, good linear dynamic range and reproducible migration times, that can be used in laboratories for high speed glycan (N-glycan) profiling applications. The CGE-based glycan analyzer will significantly increase the pace at which glycoprotein research is performed in the labs, saving hours of preparation time and assuring accurate, consistent and economical results.

Investigation of the capillary flow through open surface microfluidic structures

NASA Astrophysics Data System (ADS)

Taher, Ahmed; Jones, Benjamin; Fiorini, Paolo; Lagae, Liesbet

2017-02-01

The passive nature of capillary microfluidics for pumping and actuation of fluids is attractive for many applications including point of care medical diagnostics. For such applications, there is often the need to spot dried chemical reagents in the bottom of microfluidic channels after device fabrication; it is often more practical to have open surface devices (i.e., without a cover or lid). However, the dynamics of capillary driven flow in open surface devices have not been well studied for many geometries of interest. In this paper, we investigate capillary flow in an open surface microchannel with a backward facing step. An analytical model is developed to calculate the capillary pressure as the liquid-vapor interface traverses a backward facing step in an open microchannel. The developed model is validated against results from Surface Evolver liquid-vapor surface simulations and ANSYS Fluent two-phase flow simulations using the volume of fluid approach. Three different aspect ratios (inlet channel height by channel width) were studied. The analytical model shows good agreement with the simulation results from both modeling methods for all geometries. The analytical model is used to derive an expression for the critical aspect ratio (the minimum channel aspect ratio for flow to proceed across the backward facing step) as a function of contact angle.

Measurements and simulation of liquid films during drainage displacements and snap-off in constricted capillary tubes.

PubMed

Roman, Sophie; Abu-Al-Saud, Moataz O; Tokunaga, Tetsu; Wan, Jiamin; Kovscek, Anthony R; Tchelepi, Hamdi A

2017-12-01

When a wetting liquid is displaced by air in a capillary tube, a wetting film develops between the tube wall and the air that is responsible for the snap-off mechanism of the gas phase. By dissolving a dye in the wetting phase it is possible to relate a measure of the absorbance in the capillary to the thickness of liquid films. These data could be used to compare with cutting edge numerical simulations of the dynamics of snap-off for which experimental and numerical data are lacking. Drainage experiments in constricted capillary tubes were performed where a dyed wetting liquid is displaced by air for varying flow rates. We developed an optical method to measure liquid film thicknesses that range from 3 to 1000μm. The optical measures are validated by comparison with both theory and direct numerical simulations. In a constricted capillary tube we observed, both experimentally and numerically, a phenomenon of snap-off coalescence events in the vicinity of the constriction that bring new insights into our understanding and modeling of two-phase flows. In addition, the good agreement between experiments and numerical simulations gives confidence to use the numerical method for more complex geometries in the future. Copyright © 2017 Elsevier Inc. All rights reserved.

Intermittent Surface Water Connectivity: Fill and Spill Vs. Fill and Merge Dynamics.

EPA Science Inventory

Intermittent surface connectivity can influence aquatic systems, since chemical and biotic movements are often associated with water flow. Although often referred to as fill and spill, wetlands also fill and merge. We examined the effects of these connection types on water level...

Intermittent Surface Water Connectivity: Fill and Spill vs. Fill and Merge Dynamics

EPA Science Inventory

Intermittent surface connectivity can influence aquatic systems, since chemical and biotic movements are often associated with water flow. Although often referred to as fill and spill, wetlands also fill and merge. We examined the effects of these connection types on water level...

Longitudinal imaging of microvascular remodelling in proliferative diabetic retinopathy using adaptive optics scanning light ophthalmoscopy.

PubMed

Chui, Toco Yuen Ping; Pinhas, Alexander; Gan, Alexander; Razeen, Moataz; Shah, Nishit; Cheang, Eric; Liu, Chun L; Dubra, Alfredo; Rosen, Richard B

2016-05-01

To characterise longitudinal changes in the retinal microvasculature of type 2 diabetes mellitus (T2DM) as exemplified in a patient with proliferative diabetic retinopathy (PDR) using an adaptive optics scanning light ophthalmoscope (AOSLO). A 35-year-old T2DM patient with PDR treated with scatter pan-retinal photocoagulation at the inferior retina 1 day prior to initial AOSLO imaging along with a 24-year-old healthy control were imaged in this study. AOSLO vascular structural and perfusion maps were acquired at four visits over a 20-week period. Capillary diameter and microaneurysm area changes were measured on the AOSLO structural maps. Imaging repeatability was established using longitudinal imaging of microvasculature in the healthy control. Capillary occlusion and recanalisation, capillary dilatation, resolution of local retinal haemorrhage, capillary hairpin formation, capillary bend formation, microaneurysm formation, progression and regression were documented over time in a region 2° superior to the fovea in the PDR patient. An identical microvascular network with same capillary diameter was observed in the control subject over time. High-resolution serial AOSLO imaging enables in vivo observation of vasculopathic changes seen in diabetes mellitus. The implications of this methodology are significant, providing the opportunity for studying the dynamics of the pathological process, as well as the possibility of identifying highly sensitive and non-invasive biomarkers of end organ damage and response to treatment. © 2016 The Authors Ophthalmic & Physiological Optics © 2016 The College of Optometrists.

IB-LBM simulation of the haemocyte dynamics in a stenotic capillary.

PubMed

Yuan-Qing, Xu; Xiao-Ying, Tang; Fang-Bao, Tian; Yu-Hua, Peng; Yong, Xu; Yan-Jun, Zeng

2014-01-01

To study the behaviour of a haemocyte when crossing a stenotic capillary, the immersed boundary-lattice Boltzmann method was used to establish a quantitative analysis model. The haemocyte was assumed to be spherical and to have an elastic cell membrane, which can be driven by blood flow to adopt a highly deformable character. In the stenotic capillary, the spherical blood cell was stressed both by the flow and the wall dimension, and the cell shape was forced to be stretched to cross the stenosis. Our simulation investigated the haemocyte crossing process in detail. The velocity and pressure were anatomised to obtain information on how blood flows through a capillary and to estimate the degree of cell damage caused by excessive pressure. Quantitative velocity analysis results demonstrated that a large haemocyte crossing a small stenosis would have a noticeable effect on blood flow, while quantitative pressure distribution analysis results indicated that the crossing process would produce a special pressure distribution in the cell interior and to some extent a sudden change between the cell interior and the surrounding plasma.

Label free measurement of retinal blood cell flux, velocity, hematocrit and capillary width in the living mouse eye

PubMed Central

Guevara-Torres, A.; Joseph, A.; Schallek, J. B.

2016-01-01

Measuring blood cell dynamics within the capillaries of the living eye provides crucial information regarding the health of the microvascular network. To date, the study of single blood cell movement in this network has been obscured by optical aberrations, hindered by weak optical contrast, and often required injection of exogenous fluorescent dyes to perform measurements. Here we present a new strategy to non-invasively image single blood cells in the living mouse eye without contrast agents. Eye aberrations were corrected with an adaptive optics camera coupled with a fast, 15 kHz scanned beam orthogonal to a capillary of interest. Blood cells were imaged as they flowed past a near infrared imaging beam to which the eye is relatively insensitive. Optical contrast of cells was optimized using differential scatter of blood cells in the split-detector imaging configuration. Combined, these strategies provide label-free, non-invasive imaging of blood cells in the retina as they travel in single file in capillaries, enabling determination of cell flux, morphology, class, velocity, and rheology at the single cell level. PMID:27867728

Toward direct pore-scale modeling of three-phase displacements

NASA Astrophysics Data System (ADS)

Mohammadmoradi, Peyman; Kantzas, Apostolos

2017-12-01

A stable spreading film between water and gas can extract a significant amount of bypassed non-aqueous phase liquid (NAPL) through immiscible three-phase gas/water injection cycles. In this study, the pore-scale displacement mechanisms by which NAPL is mobilized are incorporated into a three-dimensional pore morphology-based model under water-wet and capillary equilibrium conditions. The approach is pixel-based and the sequence of invasions is determined by the fluids' connectivity and the threshold capillary pressure of the advancing interfaces. In addition to the determination of three-phase spatial saturation profiles, residuals, and capillary pressure curves, dynamic finite element simulations are utilized to predict the effective permeabilities of the rock microtomographic images as reasonable representations of the geological formations under study. All the influential features during immiscible fluid flow in pore-level domains including wetting and spreading films, saturation hysteresis, capillary trapping, connectivity, and interface development strategies are taken into account. The capabilities of the model are demonstrated by the successful prediction of saturation functions for Berea sandstone and the accurate reconstruction of three-phase fluid occupancies through a micromodel.

Label free measurement of retinal blood cell flux, velocity, hematocrit and capillary width in the living mouse eye.

PubMed

Guevara-Torres, A; Joseph, A; Schallek, J B

2016-10-01

Measuring blood cell dynamics within the capillaries of the living eye provides crucial information regarding the health of the microvascular network. To date, the study of single blood cell movement in this network has been obscured by optical aberrations, hindered by weak optical contrast, and often required injection of exogenous fluorescent dyes to perform measurements. Here we present a new strategy to non-invasively image single blood cells in the living mouse eye without contrast agents. Eye aberrations were corrected with an adaptive optics camera coupled with a fast, 15 kHz scanned beam orthogonal to a capillary of interest. Blood cells were imaged as they flowed past a near infrared imaging beam to which the eye is relatively insensitive. Optical contrast of cells was optimized using differential scatter of blood cells in the split-detector imaging configuration. Combined, these strategies provide label-free, non-invasive imaging of blood cells in the retina as they travel in single file in capillaries, enabling determination of cell flux, morphology, class, velocity, and rheology at the single cell level.

Dynamic responses of concrete-filled steel tubular member under axial compression considering creep effect

NASA Astrophysics Data System (ADS)

Jiang, X. T.; Wang, Y. D.; Dai, C. H.; Ding, M.

2017-08-01

The finite element model of concrete-filled steel tubular member was established by the numerical analysis software considering material nonlinearity to analyze concrete creep effect on the dynamic responses of the member under axial compression and lateral impact. In the model, the constitutive model of core concrete is the plastic damage model, that of steel is the Von Mises yield criterion and kinematic hardening model, and the creep effect at different ages is equivalent to the change of concrete elastic modulus. Then the dynamic responses of concrete-filled steel tubular member considering creep effects was simulated, and the effects of creep on contact time, impact load, deflection, stress and strain were discussed. The fruits provide a scientific basis for the design of the impact resistance of concrete filled steel tubular members.

Analysis of anabolic androgenic steroids in urine by full-capillary sample injection combined with a sweeping CE stacking method.

PubMed

Wang, Chun-Chi; Cheng, Shu-Fang; Cheng, Hui-Ling; Chen, Yen-Ling

2013-02-01

This study describes an on-line stacking CE approach by sweeping with whole capillary sample filling for analyzing five anabolic androgenic steroids in urine samples. The five anabolic steroids for detection were androstenedione, testosterone, epitestosterone, boldenone, and clostebol. Anabolic androgenic steroids are abused in sport doping because they can promote muscle growth. Therefore, a sensitive detection method is imperatively required for monitoring the urine samples of athletes. In this research, an interesting and reliable stacking capillary electrophoresis method was established for analysis of anabolic steroids in urine. After liquid-liquid extraction by n-hexane, the supernatant was dried and reconstituted with 30 mM phosphate buffer (pH 5.00) and loaded into the capillary by hydrodynamic injection (10 psi, 99.9 s). The stacking and separation were simultaneously accomplished at -20 kV in phosphate buffer (30 mM, pH 5.0) containing 100 mM sodium dodecyl sulfate and 40 % methanol. During the method validation, calibration curves were linear (r≥0.990) over a range of 50-1,000 ng/mL for the five analytes. In the evaluation of precision and accuracy for this method, the absolute values of the RSD and the RE in the intra-day (n=3) and inter-day (n=5) analyses were all less than 6.6 %. The limit of detection for the five analytes was 30 ng/mL (S/N=5, sampling 99.9 s at 10 psi). Compared with simple MECK, this stacking method possessed a 108- to 175-fold increase in sensitivity. This simple and sensitive stacking method could be used as a powerful tool for monitoring the illegal use of doping.

An experimental investigation of bubble splitting through multiple bifurcations

NASA Astrophysics Data System (ADS)

Bull, Joseph L.; Eshpuniyani, Brijesh; Fowlkes, J. Brian

2004-11-01

A bench top vascular bifurcation model is used to investigate the splitting of long bubbles in a series of liquid-filled bifurcations. These experiments are motivated by a gas embolotherapy technique for the potential treatment of cancer by using gas emboli to infarct tumors. The gas bubbles originate as perfluorocarbon droplets that are small enough to pass through capillaries and are injected into the bloodstream. Low intensity ultrasound is used to track their motion, and they are vaporized at the desired location for treatment via high intensity ultrasound to produce gas bubbles whose volumes are approximately 125 to 150 times the droplet volume. Achieving complete tumor necrosis requires infarction of most of the tumor. Understanding the transport and splitting of the gas bubbles, which can be long enough to extend through more than one bifurcation, is necessary to design delivery strategies. The current experiments investigate the behavior of a bubble as it passes through a series of two geometrically symmetric bifurcations, for different values of effective Bond number, which depends on gravity and the positioning of the bifurcation, capillary number, and bubble volume. The experiments are designed to match the Reynolds, Bond and capillary numbers to the physiological values for arterioles, and to provide guidance in achieving uniform tumor infarction. This work is supported by NSF grant BES-0301278 and NIH grant EB003541-01.

Screening HIV-1 fusion inhibitors based on capillary electrophoresis head-end microreactor targeting to the core structure of gp41.

PubMed

Liu, Lihong; Xu, Xiaoying; Liu, Yanhui; Zhang, Xuanxuan; Li, Lin; Jia, Zhimin

2016-02-20

In this paper, we design a microreactor based on electrophoretically mediated microanalysis (EMMA) with capillary electrophoresis (CE) for screening HIV-1 inhibitors that bind to the N-terminal heptad repeat (NHR, N36) region. Initially, a test sample plug is loaded into a capillary filled with buffer solution followed by N36 peptide solution, and the two solutions simultaneously mix by diffusion. Then, voltage is applied, and the sample molecules pass through the N36 peptide zone. The active compounds combine with N36, leading to a loss in the peak height of the active compound. More than 100 traditional Chinese medicine extracts (TCME) were screened, and an extract of Pheretima aspergillum (E. Perrier) (L5) was identified as having potent inhibitory activity. The results showed that L5 could significantly inhibit the HIV-1JR-FL pseudotyped virus infection; the 50% effective concentration (EC50) of L5 was approximately 32.1±1.2μg/mL, and the 50% cytotoxicity concentration (CC50) value of L5 was 146.9±4.4μg/mL, suggesting that L5 had low in vitro cytotoxicity on U87-CD4-CCR5 cells. The new method is simple and rapid, is free of antibodies, and does not require tedious processes. Copyright © 2015 Elsevier B.V. All rights reserved.

Experiment K-7-28: Lung Morphology Study

NASA Technical Reports Server (NTRS)

West, J. B.; Elliott, A. R.; Mathieu-Costello, O.; Kaplansky, A. S.

1994-01-01

There are no previous studies investigating the effect of microgravity exposure during spaceflight on lung tissue. We examined the ultrastructure of the left lungs of 5 Czechoslovakian Wistar rats flown on the 13 day, 19+ hr. Cosmos 2044 mission, and compared them to 5 vivarium and 5 synchronous controls at 1-g conditions, and 5 rats exposed to 14 days of tail-suspension. Within 10 minutes of sacrifice by decapitation, the lungs were removed and immersed in 3% glutaraldehyde in 0.1M phosphate buffer (total osmolarity of the fixative: 560 mOsm; pH = 7.4). The tissue stored at 5 C was transported to our laboratory where it was processed for light and electron microscopy. No significant perivascular cuffing caused by interstitial edema was present in the tissue samples. Some of the flight, tail-suspended, and synchronous control rats showed alveolar edema, while vivarium controls did not. The pulmonary capillaries appeared to be more congested in the flight animals than in the other groups. This could be related to the increased hematocrit due to the microgravity exposure. In all 5 flight, 4 tail-suspended, and 3 synchronous rats, red blood cells (RBC) were present in the alveolar spaces. The RBC were either suspended free in the alveoli or observed lining the alveolar wall. The frequency of RBC lining the alveolar walls appeared greater in the dorsal (gravity non-dependent) than in ventral (gravity dependent) regions of the lung in these three animal groups. In 3 of the vivarium controls, a few RBC were found in the alveolar spaces. Intra-capillary fluid-filled vesicles were observed in the flight, tail-suspended and synchronous animals, but not in the vivarium controls. The formation of intra-capillary fluid-filled vesicles has been previously associated with pulmonary hypertension induced by high altitude exposure and mitral stenosis. In conclusion, pulmonary hemorrhage and alveolar edema of unknown origin occurred to a greater extent in the flight, tail-suspended, and synchronous control animals, and in the dorsal regions of the lung when compared to the vivarium controls. The etiology of these changes, which are possibly due to an increase in pulmonary vascular pressure, requires further investigation.

Influence of local capillary trapping on containment system effectiveness

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bryant, Steven

2014-03-31

Immobilization of CO 2 injected into deep subsurface storage reservoirs is a critical component of risk assessment for geologic CO 2 storage (GCS). Local capillary trapping (LCT) is a recently established mode of immobilization that arises when CO 2 migrates due to buoyancy through heterogeneous storage reservoirs. This project sought to assess the amount and extent of LCT expected in storage formations under a range of injection conditions, and to confirm the persistence of LCT if the seal overlying the reservoir were to lose its integrity. Numerical simulation using commercial reservoir simulation software was conducted to assess the influence ofmore » injection. Laboratory experiments, modeling and numerical simulation were conducted to assess the effect of compromised seal integrity. Bench-scale (0.6 m by 0.6 m by 0.03 m) experiments with surrogate fluids provided the first empirical confirmation of the key concepts underlying LCT: accumulation of buoyant nonwetting phase at above residual saturations beneath capillary barriers in a variety of structures, which remains immobile under normal capillary pressure gradients. Immobilization of above-residual saturations is a critical distinction between LCT and the more familiar “residual saturation trapping.” To estimate the possible extent of LCT in a storage reservoir an algorithm was developed to identify all potential local traps, given the spatial distribution of capillary entry pressure in the reservoir. The algorithm assumes that the driving force for CO 2 migration can be represented as a single value of “critical capillary entry pressure” P c,entry crit, such that cells with capillary entry pressure greater/less than P c,entry crit act as barriers/potential traps during CO 2 migration. At intermediate values of P c,entry crit, the barrier regions become more laterally extensive in the reservoir, approaching a percolation threshold while non-barrier regions remain numerous. The maximum possible extent of LCT thus occurs at P c,entry crit near this threshold. Testing predictions of this simple algorithm against full-physics simulations of buoyancy-driven CO 2 migration support the concept of critical capillary entry pressure. However, further research is needed to determine whether a single value of critical capillary entry pressure always applies and how that value can be determined a priori. Simulations of injection into high-resolution (cells 0.3 m on a side) 2D and 3D heterogeneous domains show two characteristic behaviors. At small gravity numbers (vertical flow velocity much less than horizontal flow velocity) the CO 2 fills local traps as well as regions that would act as local barriers if CO 2 were moving only due to buoyancy. When injection ceases, the CO 2 migrates vertically to establish large saturations within local traps and residual saturation elsewhere. At large gravity numbers, the CO 2 invades a smaller portion of the perforated interval. Within this smaller swept zone the local barriers are not invaded, but local traps are filled to large saturation during injection and remain during post-injection gravity-driven migration. The small gravity number behavior is expected in the region within 100 m of a vertical injection well at anticipated rates of injection for commercial GCS. Simulations of leakage scenarios (through-going region of large permeability imposed in overlying seal) indicate that LCT persists (i.e. CO 2 remains held in a large fraction of the local iv traps) and the persistence is independent of injection rate during storage. Simulations of leakage for the limiting case of CO 2 migrating vertically from an areally extensive emplacement in the lower portion of a reservoir showed similar strong persistence of LCT. This research has two broad implications for GCS. The first is that LCT can retain a significant fraction of the CO 2 stored in a reservoir – above and beyond the residual saturation -- if the overlying seal were to fail. Thus frameworks for risk assessment should be extended to account for LCT. The second implication is that compared to pressure driven flow in reservoirs, CO 2 migration and trapping behave in a qualitatively different manner in heterogeneous reservoirs when buoyancy is the dominant driving force for flow. Thus simulations of GCS that neglect capillary heterogeneity will fail to capture important features of the CO 2 plume. While commercial reservoir simulation software can account for fine scale capillary heterogeneity, it has not been designed to work efficiently with such domains, and no simulators can handle fine-scale resolution throughout the reservoir. A possible way to upscale the migration and trapping is to apply an “effective residual saturation” to coarse-scale grids. While the extent of overall immobilization can be correlated in this way, all coarser grids failed to capture the distance traveled by the migrating CO 2 for large gravity number. Thus it remains unclear how best to account for LCT in the routine simulation work-flow that will be needed for large-scale GCS. Alternatives meriting investigation include streamline methods, reduced-physics proxies (e.g. particle tracking), and biased invasion percolation algorithms, which are based on precisely the capillary heterogeneity essential for LCT.« less

Effect of filling ratio and orientation on the thermal performance of closed loop pulsating heat pipe using ethanol

NASA Astrophysics Data System (ADS)

Rahman, Md. Lutfor; Chowdhury, Mehrin; Islam, Nawshad Arslan; Mufti, Sayed Muhammad; Ali, Mohammad

2016-07-01

Pulsating heat pipe (PHP) is a new, promising yet ambiguous technology for effective heat transfer of microelectronic devices where heat is carried by the vapor plugs and liquid slugs of the working fluid. The aim of this research paper is to better understand the operation of PHP through experimental investigations and obtain comparative results for different parameters. A series of experiments are conducted on a closed loop PHP (CLPHP) with 8 loops made of copper capillary tube of 2 mm inner diameter. Ethanol is taken as the working fluid. The operating characteristics are studied for the variation of heat input, filling ratio (FR) and orientation. The filling ratios are 40%, 50%, 60% and 70% based on its total volume. The orientations are 0° (vertical), 30°, 45° and 60°. The results clearly demonstrate the effect of filling ratio and inclination angle on the performance, operational stability and heat transfer capability of ethanol as working fluid of CLPHP. Important insight of the operational characteristics of CLPHP is obtained and optimum performance of CLPHP using ethanol is thus identified. Ethanol works best at 50-60%FR at wide range of heat inputs. At very low heat inputs, 40%FR can be used for attaining a good performance. Filling ratio below 40%FR is not suitable for using in CLPHP as it gives a low performance. The optimum performance of the device can be obtained at vertical position.

Extra permeability is required to model dynamic oxygen measurements: evidence for functional recruitment?

PubMed Central

Barrett, Matthew JP; Suresh, Vinod

2013-01-01

Neural activation triggers a rapid, focal increase in blood flow and thus oxygen delivery. Local oxygen consumption also increases, although not to the same extent as oxygen delivery. This ‘uncoupling' enables a number of widely-used functional neuroimaging techniques; however, the physiologic mechanisms that govern oxygen transport under these conditions remain unclear. Here, we explore this dynamic process using a new mathematical model. Motivated by experimental observations and previous modeling, we hypothesized that functional recruitment of capillaries has an important role during neural activation. Using conventional mechanisms alone, the model predictions were inconsistent with in vivo measurements of oxygen partial pressure. However, dynamically increasing net capillary permeability, a simple description of functional recruitment, led to predictions consistent with the data. Increasing permeability in all vessel types had the same effect, but two alternative mechanisms were unable to produce predictions consistent with the data. These results are further evidence that conventional models of oxygen transport are not sufficient to predict dynamic experimental data. The data and modeling suggest that it is necessary to include a mechanism that dynamically increases net vascular permeability. While the model cannot distinguish between the different possibilities, we speculate that functional recruitment could have this effect in vivo. PMID:23673433

Break-up dynamics of fluctuating liquid threads

PubMed Central

Petit, Julien; Rivière, David; Kellay, Hamid; Delville, Jean-Pierre

2012-01-01

The thinning dynamics of a liquid neck before break-up, as may happen when a drop detaches from a faucet or a capillary, follows different rules and dynamic scaling laws depending on the importance of inertia, viscous stresses, or capillary forces. If now the thinning neck reaches dimensions comparable to the thermally excited interfacial fluctuations, as for nanojet break-up or the fragmentation of thermally annealed nanowires, these fluctuations should play a dominant role according to recent theory and observations. Using near-critical interfaces, we here fully characterize the universal dynamics of this thermal fluctuation-dominated regime and demonstrate that the cross-over from the classical two-fluid pinch-off scenario of a liquid thread to the fluctuation-dominated regime occurs at a well-defined neck radius proportional to the thermal length scale. Investigating satellite drop formation, we also show that at the level of the cross-over between these two regimes it is more probable to produce monodisperse droplets because fluctuation-dominated pinch-off may allow the unique situation where satellite drop formation can be inhibited. Nonetheless, the interplay between the evolution of the neck profiles from the classical to the fluctuation-dominated regime and the satellites’ production remains to be clarified. PMID:23090994

Two reference time scales for studying the dynamic cavitation of liquid films

NASA Technical Reports Server (NTRS)

Sun, D. C.; Brewe, D. E.

1992-01-01

Two formulas, one for the characteristic time of filling a void with the vapor of the surrounding liquid, and one of filling the void by diffusion of the dissolved gas in the liquid, are derived. By comparing these time scales with that of the dynamic operation of oil film bearings, it is concluded that the evaporation process is usually fast enough to fill the cavitation bubble with oil vapor; whereas the diffusion process is much too slow for the dissolved air to liberate itself and enter the cavitation bubble. These results imply that the formation of a two phase fluid in dynamically loaded bearings, as often reported in the literature, is caused by air entrainment. They further indicate a way to simplify the treatment of the dynamic problem of bubble evolution.

Advances in Spacecraft Brine Water Recovery: Development of a Radial Vaned Capillary Drying Tray

NASA Technical Reports Server (NTRS)

Callahan, Michael R.; Sargusingh, Miriam J.; Pickering, Karen D.; Weislogel, Mark M.

2014-01-01

Technology improvements in the recovery of water from brine are critical to establishing closed-loop water recovery systems, enabling long-duration missions, and achieving a sustained human presence in space. A genre of 'in-place drying' brine water recovery concepts, collectively referred to herein as Brine Residual In-Containment, are under development. These brine water recovery concepts aim to increase the overall robustness and reliability of the brine recovery process by performing drying inside the container used for final disposal of the solid residual waste. Implementation of in-place drying techniques have been demonstrated for applications where gravity is present and phase separation occurs naturally by buoyancy-induced effects. In this work, a microgravity-compatible analogue of the gravity-driven phase separation process is considered by exploiting capillarity in the form of surface wetting, surface tension, and container geometry. The proposed design consists of a series of planar radial vanes aligned about a central slotted core. Preliminary testing of the fundamental geometry in a reduced gravity environment has shown the device to spontaneously fill and saturate rapidly, thereby creating a free surface from which evaporation and phase separation can occur similar to a terrestrial-like 'cylindrical pool' of fluid. Mathematical modeling and analysis of the design suggest predictable rates of filling and stability of fluid containment as a function of relevant system dimensions; e.g., number of vanes, vane length, width, and thickness. A description of the proposed capillary design solution is presented along with preliminary results from testing, modeling, and analysis of the system.

Advancements in Spacecraft Brine Water Recovery: Development of a Radial Vaned Capillary Drying Tray

NASA Technical Reports Server (NTRS)

Callahan, Michael R.; Sargusingh, Miriam J.; Pickerin, Karen D.; Weislogel, Mark M.

2013-01-01

Technology improvements in the recovery of water from brine are critical to establishing closedloop water recovery systems, enabling long duration missions, and achieving a sustained human presence in space. A genre of 'in-place drying' brine water recovery concepts, collectively referred to herein as Brine Residual In-Containment (BRIC), are under development which aim to increase the overall robustness and reliability of the brine recovery process by performing drying inside the container used for final disposal of the solid residual waste. Implementation of in-place drying techniques have been demonstrated for applications where gravity is present and phase separation occurs naturally by buoyancy induced effects. In this work, a microgravity compatible analogue of the gravity-driven phase separation process is considered by exploiting capillarity in the form of surface wetting, surface tension, and container geometry. The proposed design consists of a series of planar radial vanes aligned about a central slotted core. Preliminary testing of the fundamental geometry in a reduced gravity environment has shown the device to spontaneously fill and saturate rapidly creating a free surface from which evaporation and phase separation can occur similar to a 1-g like 'cylindrical pool' of fluid. Mathematical modeling and analysis of the design suggest predictable rates of filling and stability of fluid containment as a function of relevant system dimensions, e.g., number of vanes, vane length, width, and thickness. A description of the proposed capillary design solution is presented along with preliminary results from testing, modeling and analysis of the system.

Heat transfer within a flat micro heat pipe with extra liquid

NASA Astrophysics Data System (ADS)

Sprinceana, Silviu; Mihai, Ioan

2016-12-01

In the real functioning of flat micro heat pipe (FMHP), there can appear cases when the temperature from the vaporization zone can exceed a critical value caused by a sudden increase of the thermal flow. The heat transfer which is completed conductively through the copper wall of a FMHP vaporizer causes the vaporization of the work fluid. On the condenser, the condensation of the fluid vapors and the transfer of the condenser to the vaporizer can no longer be achieved. The solution proposed for enhancing heat transfer in the event of blockage phenomenon FMHP, it is the injection of a certain amount of working fluid in the vaporization zone. By this process the working fluid injected into the evaporator passes suddenly in the vapor, producing a cooling zone. The new product additional mass of vapor will leave the vaporization zone and will condense in condensation zone, thereby supplementing the amount of condensation. Thus resumes normal operating cycle of FMHP. For the experimental measurements made for the transfer of heat through the FMHP working fluid demineralized water, they were made two micro-capillary tubes of sintered copper layer. The first was filled with 1ml of demineralized water was dropped under vacuum until the internal pressure has reached a level of 1•104Pa. The second FMHP was filled with the same amount of working fluid was used and the same capillary inner layer over which was laid a polysynthetic material that will accrue an additional amount of fluid. In this case, the internal pressure was reduced to 1•104Pa.

Impact of biochar and root-induced changes on metal dynamics in the rhizosphere of Agrostis capillaris and Lupinus albus.

PubMed

Houben, David; Sonnet, Philippe

2015-11-01

Rhizosphere interactions are deemed to play a key role in the success of phytoremediation technologies. Here, the effects of biochar and root-induced changes in the rhizosphere of Agrostis capillaris L. and Lupinus albus L. on metal (Cd, Pb and Zn) dynamics were investigated using a biotest on a 2mm soil layer and a sequential extraction procedure (Tessier's scheme). In the bulk soil, the application of 5% biochar significantly reduced the exchangeable pool of metals primarily due to a liming effect which subsequently promoted the metal shift into the carbonate-bound pool. However, metals were re-mobilized in the rhizosphere of both A. capillaris and L. albus due to root-induced acidification which counteracted the liming effect of biochar. As a result, the concentrations of metals in roots and shoots of both plants were not significantly reduced by the application of biochar. Although the study should be considered a worst-case scenario because experimental conditions induced the intensification of rhizosphere processes, the results highlight that changes in rhizosphere pH can impact the effectiveness of biochar to immobilize metals in soil. Biochar has thus a potential as amendment for reducing metal uptake by plants, provided the acidification of the rhizosphere is minimized. Copyright © 2014 Elsevier Ltd. All rights reserved.

An Amorphous Network Model for Capillary Flow and Dispersion in a Partially Saturated Porous Medium

NASA Astrophysics Data System (ADS)

Simmons, C. S.; Rockhold, M. L.

2013-12-01

Network models of capillary flow are commonly used to represent conduction of fluids at pore scales. Typically, a flow system is described by a regular geometric lattice of interconnected tubes. Tubes constitute the pore throats, while connection junctions (nodes) are pore bodies. Such conceptualization of the geometry, however, is questionable for the pore scale, where irregularity clearly prevails, although prior published models using a regular lattice have demonstrated successful descriptions of the flow in the bulk medium. Here a network is allowed to be amorphous, and is not subject to any particular lattice structure. Few network flow models have treated partially saturated or even multiphase conditions. The research trend is toward using capillary tubes with triangular or square cross sections that have corners and always retain some fluid by capillarity when drained. In contrast, this model uses only circular capillaries, whose filled state is controlled by a capillary pressure rule for the junctions. The rule determines which capillary participate in the flow under an imposed matric potential gradient during steady flow conditions. Poiseuille's Law and Laplace equation are used to describe flow and water retention in the capillary units of the model. A modified conjugate gradient solution for steady flow that tracks which capillary in an amorphous network contribute to fluid conduction was devised for partially saturated conditions. The model thus retains the features of classical capillary models for determining hydraulic flow properties under unsaturated conditions based on distribution of non-interacting tubes, but now accounts for flow exchange at junctions. Continuity of the flow balance at every junction is solved simultaneously. The effective water retention relationship and unsaturated permeability are evaluated for an extensive enough network to represent a small bulk sample of porous medium. The model is applied for both a hypothetically randomly generate network and for a directly measured porous medium structure, by means of xray-CT scan. A randomly generated network has the benefit of providing ensemble averages for sample replicates of a medium's properties, whereas network structure measurements are expected to be more predictive. Dispersion of solute in a network flow is calculate by using particle tracking to determine the travel time breakthrough between inflow and outflow boundaries. The travel time distribution can exhibit substantial skewness that reflects both network velocity variability and mixing dilution at junctions. When local diffusion is not included, and transport is strictly advective, then the skew breakthrough is not due to mobile-immobile flow region behavior. The approach of dispersivity to its asymptotic value with sample size is examined, and may be only an indicator of particular stochastic flow variation. It is not proven that a simplified network flow model can accurately predict the hydraulic properties of a sufficiently large-size medium sample, but such a model can at least demonstrate macroscopic flow resulting from the interaction of physical processes at pore scales.

Ocean dynamics studies. [of current-wave interactions

NASA Technical Reports Server (NTRS)

1974-01-01

Both the theoretical and experimental investigations into current-wave interactions are discussed. The following three problems were studied: (1) the dispersive relation of a random gravity-capillary wave field; (2) the changes of the statistical properties of surface waves under the influence of currents; and (3) the interaction of capillary-gravity with the nonuniform currents. Wave current interaction was measured and the feasibility of using such measurements for remote sensing of surface currents was considered. A laser probe was developed to measure the surface statistics, and the possibility of using current-wave interaction as a means of current measurement was demonstrated.

Capillary Thinning of Particle-laden Drops

NASA Astrophysics Data System (ADS)

Wagoner, Brayden; Thete, Sumeet; Jahns, Matt; Doshi, Pankaj; Basaran, Osman

2015-11-01

Drop formation is central in many applications such as ink-jet printing, microfluidic devices, and atomization. During drop formation, a thinning filament is created between the about-to-form drop and the fluid hanging from the nozzle. Therefore, the physics of capillary thinning of filaments is key to understanding drop formation and has been thoroughly studied for pure Newtonian fluids. The thinning dynamics is, however, altered completely when the fluid contains particles, the physics of which is not well understood. In this work, we explore the impact of solid particles on filament thinning and drop formation by using a combination of experiments and numerical simulations.

Three-Dimensional Simulation of Liquid Drop Dynamics Within Unsaturated Vertical Hele-Shaw Cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hai Huang; Paul Meakin

A three-dimensional, multiphase fluid flow model with volume of fluid-interface tracking was developed and applied to study the multiphase dynamics of moving liquid drops of different sizes within vertical Hele-Shaw cells. The simulated moving velocities are significantly different from those obtained from a first-order analytical approximation, based on simple force-balance concepts. The simulation results also indicate that the moving drops can exhibit a variety of shapes and that the transition among these different shapes is largely determined by the moving velocities. More important, there is a transition from a linear moving regime at small capillary numbers, in which the capillarymore » number scales linearly with the Bond number, to a nonlinear moving regime at large capillary numbers, in which the moving drop releases a train of droplets from its trailing edge. The train of droplets forms a variety of patterns at different moving velocities.« less

Whole-body mathematical model for simulating intracranial pressure dynamics

NASA Technical Reports Server (NTRS)

Lakin, William D. (Inventor); Penar, Paul L. (Inventor); Stevens, Scott A. (Inventor); Tranmer, Bruce I. (Inventor)

2007-01-01

A whole-body mathematical model (10) for simulating intracranial pressure dynamics. In one embodiment, model (10) includes 17 interacting compartments, of which nine lie entirely outside of intracranial vault (14). Compartments (F) and (T) are defined to distinguish ventricular from extraventricular CSF. The vasculature of the intracranial system within cranial vault (14) is also subdivided into five compartments (A, C, P, V, and S, respectively) representing the intracranial arteries, capillaries, choroid plexus, veins, and venous sinus. The body's extracranial systemic vasculature is divided into six compartments (I, J, O, Z, D, and X, respectively) representing the arteries, capillaries, and veins of the central body and the lower body. Compartments (G) and (B) include tissue and the associated interstitial fluid in the intracranial and lower regions. Compartment (Y) is a composite involving the tissues, organs, and pulmonary circulation of the central body and compartment (M) represents the external environment.

Capillary trapping of particles in thin-film flows

NASA Astrophysics Data System (ADS)

Dressaire, Emilie; Gomez, Michael; Colnet, Benedicte; Sauret, Alban

2017-11-01

When a thin layer of suspension flows over a substrate, some particles remain trapped on the solid surface. When the thickness of the liquid layer is comparable to the particle size, the particles deform the liquid interface, which leads to local interactions. These effects modify the transport of particles and the dynamics of the liquid films. Here, we characterize how capillary interactions affect the transport and deposition of non-Brownian particles moving in thin liquid films and the resulting loss of transported material. We focus on gravitational drainage flows, in which the film thickness becomes comparable to the particle size. Depending on the concentration of particles, we find that the drainage dynamics exhibits behavior that cannot be captured with a continuum model, due to the deposition of particles on the substrate. ANR-16-CE30-0009 & CNRS-PICS-07242 & ACS-PRF 55845-ND9.

Capillary wave propagation during the delamination of graphene by the precursor films in electro-elasto-capillarity

PubMed Central

Zhu, Xueyan; Yuan, Quanzi; Zhao, Ya-Pu

2012-01-01

Molecular dynamics simulations were carried out to explore the capillary wave propagation induced by the competition between one upper precursor film (PF) on the graphene and one lower PF on the substrate in electro-elasto-capillarity (EEC). During the wave propagation, the graphene was gradually delaminated from the substrate by the lower PF. The physics of the capillary wave was explored by the molecular kinetic theory. Besides, the dispersion relation of the wave was obtained theoretically. The theory showed that the wave was controlled by the driving work difference of the two PFs. Simulating the EEC process under different electric field intensities (E), the wave velocity was found insensitive to E. We hope this research could expand our knowledge on the wetting, electrowetting and EEC. As a potential application, the electrowetting of the PF between the graphene and the substrate is a promising candidate for delaminating graphene from substrate. PMID:23226593

Investigation of transient dynamics of capillary assisted particle assembly yield

NASA Astrophysics Data System (ADS)

Virganavičius, D.; Juodėnas, M.; Tamulevičius, T.; Schift, H.; Tamulevičius, S.

2017-06-01

In this paper, the transient behavior of the particle assembly yield dynamics when switching from low yield to high yield deposition at different velocity and thermal regimes is investigated. Capillary force assisted particle assembly (CAPA) using colloidal suspension of green fluorescent 270 nm diameter polystyrene beads was performed on patterned poly (dimethyl siloxane) substrates using a custom-built deposition setup. Two types of patterns with different trapping site densities were used to assess CAPA process dynamics and the influence of pattern density and geometry on the deposition yield transitions. Closely packed 300 nm diameter circular pits ordered in hexagonal arrangement with 300 nm pitch, and 2 × 2 mm2 square pits with 2 μm spacing were used. 2-D regular structures of the deposited particles were investigated by means of optical fluorescence and scanning electron microscopy. The fluorescence micrographs were analyzed using a custom algorithm enabling to identify particles and calculate efficiency of the deposition performed at different regimes. Relationship between the spatial distribution of particles in transition zone and ambient conditions was evaluated and quantified by approximation of the yield profile with a logistic function.

Comparison of linear and nonlinear models for coherent hemodynamics spectroscopy (CHS)

NASA Astrophysics Data System (ADS)

Sassaroli, Angelo; Kainerstorfer, Jana; Fantini, Sergio

2015-03-01

A recently proposed linear time-invariant hemodynamic model for coherent hemodynamics spectroscopy1 (CHS) relates the tissue concentrations of oxy- and deoxy-hemoglobin (outputs of the system) to given dynamics of the tissue blood volume, blood flow and rate constant of oxygen diffusion (inputs of the system). This linear model was derived in the limit of "small" perturbations in blood flow velocity. We have extended this model to a more general model (which will be referred to as the nonlinear extension to the original model) that yields the time-dependent changes of oxy and deoxy-hemoglobin concentrations in response to arbitrary dynamic changes in capillary blood flow velocity. The nonlinear extension to the model relies on a general solution of the partial differential equation that governs the spatio-temporal behavior of oxygen saturation of hemoglobin in capillaries and venules on the basis of dynamic (or time resolved) blood transit time. We show preliminary results where the CHS spectra obtained from the linear and nonlinear models are compared to quantify the limits of applicability of the linear model.

Scaling law on formation and rupture of a dynamical liquid bridge

NASA Astrophysics Data System (ADS)

Zhang, Huang; Zhang, Zehao; Liu, Qianfeng; Li, Shuiqing; Department of Thermal Engineering, Tsinghua University Collaboration; Institute of Nuclear Energy; Technology, Tsinghua University Collaboration

2017-11-01

The formation and breakup of a pendular liquid bridge in dynamic state is investigated experimentally. The experimental setup arises from a system to measure the coefficient of restitution (COR) of a glass sphere impacting and bouncing on a wetted surface. We compare the effect of surface tension and gravity on the liquid bridge rupture by the capillary length κ-1. For water and liquid 1 (50% water mixed with 50% glycerol), the gravity is dominant on the liquid bridge breakup. And we find that the rupture distance is in good linear trend with the non-dimensional number G by the scaling law analysis. Further, for liquid 2 (25% water mixed with 75% glycerol) that is relatively high viscous, the linear changing of the rupture distance with the capillary number Ca is found. The relation of the rupture distance with G and Ca would be helpful in understanding the complex behavior of the dynamical liquid bridge. This work was funded by the Major State Basic Research Development Program of China (Grant No. 2016YFC0203705) and the China Postdoctoral Science Foundation (Grant No. 2016M601024).

Development of a flow controller for long-term sampling of gases and vapors using evacuated canisters.

PubMed

Rossner, Alan; Farant, Jean Pierre; Simon, Philippe; Wick, David P

2002-11-15

Anthropogenic activities contribute to the release of a wide variety of volatile organic compounds (VOC) into microenvironments. Developing and implementing new air sampling technologies that allow for the characterization of exposures to VOC can be useful for evaluating environmental and health concerns arising from such occurrences. A novel air sampler based on the use of a capillary flow controller connected to evacuated canisters (300 mL, 1 and 6 L) was designed and tested. The capillary tube, used to control the flow of air, is a variation on a sharp-edge orifice flow controller. It essentially controls the velocity of the fluid (air) as a function of the properties of the fluid, tube diameter and length. A model to predict flow rate in this dynamic system was developed. The mathematical model presented here was developed using the Hagen-Poiseuille equation and the ideal gas law to predict flow into the canisters used to sample for long periods of time. The Hagen-Poiseuille equation shows the relationship between flow rate, pressure gradient, capillary resistance, fluid viscosity, capillary length and diameter. The flow rates evaluated were extremely low, ranging from 0.05 to 1 mL min(-1). The model was compared with experimental results and was shown to overestimate the flow rate. Empirical equations were developed to more accurately predict flow for the 300 mL, 1 and 6 L canisters used for sampling periods ranging from several hours to one month. The theoretical and observed flow rates for different capillary geometries were evaluated. Each capillary flow controller geometry that was tested was found to generate very reproducible results, RSD < 2%. Also, the empirical formulas developed to predict flow rate given a specified diameter and capillary length were found to predict flow rate within 6% of the experimental data. The samplers were exposed to a variety of airborne vapors that allowed for comparison of the effectiveness of capillary flow controllers to sorbent samplers and to an online gas chromatograph. The capillary flow controller was found to exceed the performance of the sorbent samplers in this comparison.

Experimental study on nonmonotonicity of Capillary Desaturation Curves in a 2-D pore network

NASA Astrophysics Data System (ADS)

Rodríguez de Castro, Antonio; Shokri, Nima; Karadimitriou, Nikolaos; Oostrom, Mart; Joekar-Niasar, Vahid

2015-10-01

Immiscible displacement in porous media is important in many applications such as soil remediation and enhanced oil recovery. When gravitational forces are negligible, two-phase immiscible displacement at the pore level is controlled by capillary and viscous forces whose relative importance is quantified through the dimensionless capillary number Ca and the viscosity ratio M between liquid phases. Depending on the values of Ca and M, capillary fingering, viscous fingering, or stable displacement may be observed resulting in a variety of patterns affecting the phase entrapment. The Capillary Desaturation Curve (CDC), which represents the relationship between the residual oil saturation and Ca, is an important relation to describe the phase entrapment at a given Ca. In the present study, we investigated the CDC as influenced by the viscosity ratio. To do so, we have conducted a comprehensive series of experiments using a high-resolution microscope and state-of-art micromodels to investigate the dynamics and patterns of phase entrapment at different Ca and M. By postprocessing of the experimental high-resolution images, we calculated the CDC and quantified the effects of the Ca and M on the phase entrapment and number of blobs trapped in the micromodel and their size distributions during immiscible two-phase flow. Our results show that CDCs are not necessarily monotonic for all M, and the physical mechanisms causing this nonmonotonic behavior are discussed.

Defect reduction of high-density full-field patterns in jet and flash imprint lithography

NASA Astrophysics Data System (ADS)

Singh, Lovejeet; Luo, Kang; Ye, Zhengmao; Xu, Frank; Haase, Gaddi; Curran, David; LaBrake, Dwayne; Resnick, Douglas; Sreenivasan, S. V.

2011-04-01

Imprint lithography has been shown to be an effective technique for replication of nano-scale features. Jet and Flash Imprint Lithography (J-FIL) involves the field-by-field deposition and exposure of a low viscosity resist deposited by jetting technology onto the substrate. The patterned mask is lowered into the fluid which then quickly flows into the relief patterns in the mask by capillary action. Following this filling step, the resist is crosslinked under UV radiation, and then the mask is removed leaving a patterned resist on the substrate. Acceptance of imprint lithography for manufacturing will require demonstration that it can attain defect levels commensurate with the defect specifications of high end memory devices. Typical defectivity targets are on the order of 0.10/cm2. This work summarizes the results of defect inspections focusing on two key defect types; random non-fill defects occurring during the resist filling process and repeater defects caused by interactions with particles on the substrate. Non-fill defectivity must always be considered within the context of process throughput. The key limiting throughput step in an imprint process is resist filling time. As a result, it is critical to characterize the filling process by measuring non-fill defectivity as a function of fill time. Repeater defects typically have two main sources; mask defects and particle related defects. Previous studies have indicated that soft particles tend to cause non-repeating defects. Hard particles, on the other hand, can cause either resist plugging or mask damage. In this work, an Imprio 500 twenty wafer per hour (wph) development tool was used to study both defect types. By carefully controlling the volume of inkjetted resist, optimizing the drop pattern and controlling the resist fluid front during spreading, fill times of 1.5 seconds were achieved with non-fill defect levels of approximately 1.2/cm2. Longevity runs were used to study repeater defects and a nickel contamination was identified as the key source of particle induced repeater defects.

Modeling of FMISO [F18] nanoparticle PET tracer in normal-cancerous tissue based on real clinical image.

PubMed

Asgari, Hanie; Soltani, M; Sefidgar, Mostafa

2018-07-01

Hypoxia as one of the principal properties of tumor cells is a reaction to the deprivation of oxygen. The location of tumor cells could be identified by assessment of oxygen and nutrient level in human body. Positron emission tomography (PET) is a well-known non-invasive method that is able to measure hypoxia based on the FMISO (Fluoromisonidazole) tracer dynamic. This paper aims to study the PET tracer concentration through convection-diffusion-reaction equations in a real human capillary-like network. A non-uniform oxygen pressure along the capillary path and convection mechanism for FMISO transport are taken into account to accurately model the characteristics of the tracer. To this end, a multi-scale model consists of laminar blood flow through the capillary network, interstitial pressure, oxygen pressure, FMISO diffusion and FMISO convection transport in the extravascular region is developed. The present model considers both normal and tumor tissue regions in computational domain. The accuracy of numerical model is verified with the experimental results available in the literature. The convection and diffusion types of transport mechanism are employed in order to calculate the concentration of FMISO in the normal and tumor sub-domain. The influences of intravascular oxygen pressure, FMISO transport mechanisms, capillary density and different types of tissue on the FMISO concentration have been investigated. According to result (Table 4) the convection mechanism of FMISO molecules transportation is negligible, but it causes more accuracy of the proposed model. The approach of present study can be employed in order to investigate the effects of various parameters, such as tumor shape, on the dynamic behavior of different PET tracers, such as FDG, can be extended to different case study problems, such as drug delivery. Copyright © 2018 Elsevier Inc. All rights reserved.

Effects of membrane deformability and bond formation/dissociation rates on adhesion dynamics of a spherical capsule in shear flow.

PubMed

Zhang, Ziying; Du, Jun; Wei, Zhengying; Wang, Zhen; Li, Minghui

2018-02-01

Cellular adhesion plays a critical role in biological systems and biomedical applications. Cell deformation and biophysical properties of adhesion molecules are of significance for the adhesion behavior. In the present work, dynamic adhesion of a deformable capsule to a planar substrate, in a linear shear flow, is numerically simulated to investigate the combined influence of membrane deformability (quantified by the capillary number) and bond formation/dissociation rates on the adhesion behavior. The computational model is based on the immersed boundary-lattice Boltzmann method for the capsule-fluid interaction and a probabilistic adhesion model for the capsule-substrate interaction. Three distinct adhesion states, detachment, rolling adhesion and firm adhesion, are identified and presented in a state diagram as a function of capillary number and bond dissociation rate. The impact of bond formation rate on the state diagram is further investigated. Results show that the critical bond dissociation rate for the transition of rolling or firm adhesion to detachment is strongly related to the capsule deformability. At the rolling-adhesion state, smaller off rates are needed for larger capillary number to increase the rolling velocity and detach the capsule. In contrast, the critical off rate for firm-to-detach transition slightly increases with the capillary number. With smaller on rate, the effect of capsule deformability on the critical off rates is more pronounced and capsules with moderate deformability are prone to detach by the shear flow. Further increasing of on rate leads to large expansion of both rolling-adhesion and firm-adhesion regions. Even capsules with relatively large deformability can maintain stable rolling adhesion at certain off rate.

Alignment-enhancing feed-through conductors for stackable silicon-on-sapphire wafers

NASA Technical Reports Server (NTRS)

Anthony, Thomas R. (Inventor)

1983-01-01

Alignment-enhancing electrically conductive feed-through paths are provided for the high-speed low-loss transfer of electrical signals between integrated circuits of a plurality of silicon-on-sapphire bodies arrayed in a stack. The alignment-enhancing feed-throughs are made by a process involving the drilling of holes through the body, double-sided sputtering, electroplating, and the filling of the holes with solder by capillary action. The alignment-enhancing feed-throughs are activated by forming a stack of wafers and remelting the solder whereupon the wafers, and the feed-through paths, are pulled into alignment by surface tension forces.

Tunable multimode-interference bandpass fiber filter.

PubMed

Antonio-Lopez, J E; Castillo-Guzman, A; May-Arrioja, D A; Selvas-Aguilar, R; Likamwa, P

2010-02-01

We report on a wavelength-tunable filter based on multimode interference (MMI) effects. A typical MMI filter consists of a multimode fiber (MMF) spliced between two single-mode fibers (SMF). The peak wavelength response of the filter exhibits a linear dependence when the length of the MMF is modified. Therefore a capillary tube filled with refractive-index-matching liquid is used to effectively increase the length of the MMF, and thus wavelength tuning is achieved. Using this filter a ring-based tunable erbium-doped fiber laser is demonstrated with a tunability of 30 nm, covering the full C-band.

Development of 3D out-of-plane SU-8 microlenses using modified micromolding in capillaries (MIMIC) technology

NASA Astrophysics Data System (ADS)

Llobera, A.; Wilke, R.; Johnson, D. W.; Büttgenbach, S.

2006-04-01

This paper describes a modification of the standard MIMIC technology, solving its main drawbacks, to define arrays of spherical or ellipsoidal microlenses. Perfectly symmetrical meniscuses have been obtained by using a XP SU-8 NO-2 layer beneath the PDMS mold. Moreover, the photostructurable properties of this polymer allow obtaining self-alignment structures for adequate fiber optics positioning. Microchannels ended with these meniscuses have been filled with standard SU-8 to obtain 3D microlenses. Agreement between theory and experimental results allows confirming the validity of the proposed technology.

Dynamic Moisture Sorption and Desorption in Fumed Silica-filled Silicone Foam

DOE Office of Scientific and Technical Information (OSTI.GOV)

Trautschold, Olivia Carol

Characterizing dynamic moisture sorption and desorption in fumed silica-filled silicone foam is necessary for determining material compatibilities and life predictions, particularly in sealed environments that may be exposed to a range of environmental conditions. Thermogravimetric analysis (TGA) and near infrared spectroscopy (NIR) were performed on S5470 fumed silica-filled silicone foam to determine the weight percent of moisture at saturation. Additionally, TGA was used to determine the time, temperature, and relative humidity levels required for sorption and desorption of physisorbed moisture in S5470.

Two-phase computed tomography study of warthin tumor of parotid gland: differentiation from other parotid gland tumors and its pathologic explanation.

PubMed

Woo, Seung Hoon; Choi, Dae-Seob; Kim, Jin-pyeong; Park, Jung Je; Joo, Yeon Hee; Chung, Phil-Sang; Kim, Bo-Young; Ko, Young-Hyeh; Jeong, Han-Sin; Kim, Hyung-Jin

2013-01-01

The objective of this study was to define the radiological characteristics of 2-phase computed tomography (CT) of parotid gland Warthin tumors (WTs) with a pathologic basis for these findings. We prospectively enrolled 116 patients with parotid gland tumor who underwent preoperative 2-phase CT scans(scanning delays of 30 and 120 seconds). The attenuation changes and enhancement patterns were analyzed according to pathology. We also evaluated size-matched samples of WTs and pleomorphic adenoma by staining CD31, vascular endothelial growth factor-receptor 2, collagen IV, and smooth muscle actin. Computed tomography numbers in WTs were significantly higher than those in other tumors in early-phase scans and lower in delayed scans. Pathologically, CD31(+) blood vessel area was significantly higher in WTs than in pleomorphic adenomas. In addition, WTs had an extensive capillary network and many leaky blood vessels. The enhancement pattern of early fill-in and early washout is the typical finding of WTs on 2-phase CT scans, which may be attributed pathologically to abundant blood vessel and extensive capillary network.

Ultrastructural changes in lung tissue after acute lead intoxication in the rat.

PubMed

Kaczynska, Katarzyna; Walski, Michał; Szereda-Przestaszewska, Małgorzata

2011-01-01

Pulmonary toxicity of lead was studied in rats after an intraperitoneal administration of lead acetate at a dose of 25 mg/kg. Three consecutive days of treatment increased lead content in the whole blood to 2.1 µg/dl and in lung homogenate it attained 9.62 µg/g w.w. versus control values of 0.17 µg/dl and 0.78 µg/g w.w., respectively. At the ultrastructural level, the effects of lead toxicity were observed in lung capillaries, interstitium, epithelial cells and alveolar lining layer. Accumulation of aggregated platelets, leucocytic elements and monocytes was found within capillaries. Interstitium comprised a substantial number of collagen, elastin filaments and lipofibroblasts. Lamellar bodies of type II pneumocytes contained phospolipid lamellae, which stratified into an irregular arrangement. Pulmonary alveoli were filled with macrophages. The extracellular lining layer of lung alveoli was partially destroyed. This study provided evidence that acute lead intoxication affects the whole lung parenchyma and by impairing production of the surfactant might disturb the regular respiratory function.

A novel pneumatic micropipette aspiration method using a balance pressure model.

PubMed

Zhao, Qili; Wu, Ming; Cui, Maosheng; Qin, Yanding; Yu, Jin; Sun, Mingzhu; Zhao, Xin; Feng, Xizeng

2013-12-01

This paper presents a novel micropipette aspiration (MA) method based on a common pneumatic micro-injection system. This method is the first to quantify the influence of capillary effect on aspiration pressure using a balance pressure model, and in return, uses the capillary effect to quantify the aspiration pressure. Subsequently, the seal between the cell and the micropipette is detected to judge and exclude the ineffective MA attempts. The rationality of the balance pressure model is validated by the designed micropipette-filling experiments. Through applied to elasticity-determination of the cells with different sizes, the feasibility and versatility of this MA method are proved. With abilities to quantify aspiration pressures and detect the seam between the cell and the micropipette, our method is expected to advance the application of the commercial pneumatic injector in the MA of cells. Moreover, with the quantified volume of the liquid entering into the micropipette during MA process, our method also has a potential applicability to the study of the permeability of the cell membrane in the future.

Mechanism of metabolic stroke and spontaneous cerebral hemorrhage in glutaric aciduria type I

PubMed Central

2014-01-01

Background Metabolic stroke is the rapid onset of lasting central neurological deficit associated with decompensation of an underlying metabolic disorder. Glutaric aciduria type I (GA1) is an inherited disorder of lysine and tryptophan metabolism presenting with metabolic stroke in infancy. The clinical presentation includes bilateral striatal necrosis and spontaneous subdural and retinal hemorrhages, which has been frequently misdiagnosed as non-accidental head trauma. The mechanisms underlying metabolic stroke and spontaneous hemorrhage are poorly understood. Results Using a mouse model of GA1, we show that metabolic stroke progresses in the opposite sequence of ischemic stroke, with initial neuronal swelling and vacuole formation leading to cerebral capillary occlusion. Focal regions of cortical followed by striatal capillaries are occluded with shunting to larger non-exchange vessels leading to early filling and dilation of deep cerebral veins. Blood–brain barrier breakdown was associated with displacement of tight-junction protein Occludin. Conclusion Together the current findings illuminate the pathophysiology of metabolic stroke and vascular compromise in GA1, which may translate to other neurometabolic disorders presenting with stroke. PMID:24468193

Mechanism of metabolic stroke and spontaneous cerebral hemorrhage in glutaric aciduria type I.

PubMed

Zinnanti, William J; Lazovic, Jelena; Housman, Cathy; Antonetti, David A; Koeller, David M; Connor, James R; Steinman, Lawrence

2014-01-27

Metabolic stroke is the rapid onset of lasting central neurological deficit associated with decompensation of an underlying metabolic disorder. Glutaric aciduria type I (GA1) is an inherited disorder of lysine and tryptophan metabolism presenting with metabolic stroke in infancy. The clinical presentation includes bilateral striatal necrosis and spontaneous subdural and retinal hemorrhages, which has been frequently misdiagnosed as non-accidental head trauma. The mechanisms underlying metabolic stroke and spontaneous hemorrhage are poorly understood. Using a mouse model of GA1, we show that metabolic stroke progresses in the opposite sequence of ischemic stroke, with initial neuronal swelling and vacuole formation leading to cerebral capillary occlusion. Focal regions of cortical followed by striatal capillaries are occluded with shunting to larger non-exchange vessels leading to early filling and dilation of deep cerebral veins. Blood-brain barrier breakdown was associated with displacement of tight-junction protein Occludin. Together the current findings illuminate the pathophysiology of metabolic stroke and vascular compromise in GA1, which may translate to other neurometabolic disorders presenting with stroke.

New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide

PubMed Central

Janković, Bojan; Janković, Marija; Nikolić, Bogdan; Dimkić, Ivica; Lalević, Blažo; Raičević, Vera

2017-01-01

Proposed distributed reactivity model of dehydration for seedling parts of two various maize hybrids (ZP434, ZP704) was established. Dehydration stresses were induced thermally, which is also accompanied by response of hybrids to heat stress. It was found that an increased value of activation energy counterparts within radicle dehydration of ZP434, with a high concentration of 24-epibrassinolide (24-EBL) at elevated operating temperatures, probably causes activation of diffusion mechanisms in cutin network and may increases likelihood of formation of free volumes, large enough to accommodate diffusing molecule. Many small random effects were detected and can be correlated with micro-disturbing in a space filled with water caused by thermal gradients, increasing capillary phenomena, and which can induce thermo-capillary migration. The influence of seedling content of various sugars and minerals on dehydration was also examined. Estimated distributed reactivity models indicate a dependence of reactivity on structural arrangements, due to present interactions between water molecules and chemical species within the plant. PMID:28644899

New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide.

PubMed

Waisi, Hadi; Janković, Bojan; Janković, Marija; Nikolić, Bogdan; Dimkić, Ivica; Lalević, Blažo; Raičević, Vera

2017-01-01

Proposed distributed reactivity model of dehydration for seedling parts of two various maize hybrids (ZP434, ZP704) was established. Dehydration stresses were induced thermally, which is also accompanied by response of hybrids to heat stress. It was found that an increased value of activation energy counterparts within radicle dehydration of ZP434, with a high concentration of 24-epibrassinolide (24-EBL) at elevated operating temperatures, probably causes activation of diffusion mechanisms in cutin network and may increases likelihood of formation of free volumes, large enough to accommodate diffusing molecule. Many small random effects were detected and can be correlated with micro-disturbing in a space filled with water caused by thermal gradients, increasing capillary phenomena, and which can induce thermo-capillary migration. The influence of seedling content of various sugars and minerals on dehydration was also examined. Estimated distributed reactivity models indicate a dependence of reactivity on structural arrangements, due to present interactions between water molecules and chemical species within the plant.

Development of an on-column enrichment technique based on C18-functionalized magnetic silica nanoparticles for the determination of lidocaine in rat plasma by high performance liquid chromatography.

PubMed

Chu, Bin; Lou, Dujuan; Yu, Panfeng; Hu, Shaonan; Shen, Shun

2011-10-14

In this study, a novel on-column enrichment technique filled with C(18)-functionalized magnetic silica nanoparticles was successfully developed for the determination of lidocaine in rat plasma by high performance liquid chromatography (HPLC). The synthesized Fe(3)O(4)@SiO(2)-C(18) nanoparticles were locally packed into the capillary by the application of magnets. Lidocaine in the sample solutions pumped into the capillary tube could be easily adsorbed by Fe(3)O(4)@SiO(2)-C(18) through hydrophobic interaction by the interior C(18) groups, and eluted by acetonitrile solution. Different extraction conditions were investigated. Method validations including linear range, quantification limit, detection limit, precision, accuracy and recovery were also studied. The results showed that the proposed method based on on-column enrichment by Fe(3)O(4)@SiO(2)-C(18) was a novel, little solvent and efficient approach for the determination of lidocaine in the complex plasma samples. Copyright © 2011 Elsevier B.V. All rights reserved.

Sequence requirements of oligonucleotide chiral selectors for the capillary electrophoresis resolution of low-affinity DNA binders.

PubMed

Tohala, Luma; Oukacine, Farid; Ravelet, Corinne; Peyrin, Eric

2017-05-01

We recently reported that a great variety of DNA oligonucleotides (ONs) used as chiral selectors in partial-filling capillary electrophoresis (CE) exhibited interesting enantioresolution properties toward low-affinity DNA binders. Herein, the sequence prerequisites of ONs for the CE enantioseparation process were studied. First, the chiral resolution properties of a series of homopolymeric sequences (Poly-dT) of different lengths (from 5 to 60-mer) were investigated. It was shown that the size increase-dependent random coil-like conformation of Poly-dT favorably acted on the apparent selectivity and resolution. The base-unpairing state constituted also an important factor in the chiral resolution ability of ONs as the switch from the single-stranded to double-stranded structure was responsible for a significant decrease in the analyte selectivity range. Finally, the chemical diversity enhanced the enantioresolution ability of single-stranded ONs. The present work could lay the foundation for the design of performant ON chiral selectors for the CE separation of weak DNA binder enantiomers. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Separation of plant hormones from biofertilizer by capillary electrophoresis using a capillary coated dynamically with polycationic polymers.

PubMed

Jiang, Ting-Fu; Lv, Zhi-Hua; Wang, Yuan-Hong; Yue, Mei-E

2006-06-01

A new, simple and rapid capillary electrophoresis (CE) method, using hexadimethrine bromide (HDB) as electroosmotic flow (EOF) modifier, was developed for the identification and quantitative determination of four plant hormones, including gibberellin A3 (GA3), indole-3-acetic acid (IAA), alpha-naphthaleneacetic acid (NAA) and 4-chlorophenoxyacetic acid (4-CA). The optimum separation was achieved with 20 mM borate buffer at pH 10.00 containing 0.005% (w/v) of HDB. The applied voltage was -25 kV and the capillary temperature was kept constant at 25 degrees C. Salicylic acid was used as internal standard for quantification. The calibration dependencies exhibited good linearity within the ratios of the concentrations of standard samples and internal standard and the ratios of the peak areas of samples and internal standard. The correlation coefficients were from 0.9952 to 0.9997. The relative standard deviations of migration times and peak areas were < 1.93 and 6.84%, respectively. The effects of buffer pH, the concentration of HDB and the voltage on the resolution were studied systematically. By this method, the contents of plant hormone in biofertilizer were successfully determined within 7 min, with satisfactory repeatability and recovery.

Steadily oscillating axial bands of binary granules in a nearly filled coaxial cylinder.

PubMed

Inagaki, Shio; Ebata, Hiroyuki; Yoshikawa, Kenichi

2015-01-01

Granular materials often segregate under mechanical agitation such as flowing, shaking, or rotating, in contrast to an expectation of mixing. It is well known that bidisperse mixtures of granular materials in a partially filled rotating cylinder exhibit monotonic coarsening dynamics of segregation. Here we report the steady oscillation of segregated axial bands under the stationary rotation of a nearly filled coaxial cylinder for O(10(3)) revolutions. The axial bands demonstrate steady back-and-forth motion along the axis of rotation. Experimental findings indicated that these axial band dynamics are driven by global convection throughout the system. The essential features of the spatiotemporal dynamics are reproduced with a simple phenomenological equation that incorporates the effect of global convection.

Dynamic mechanical analysis of waste tyre rubber filled brake friction composite materials

NASA Astrophysics Data System (ADS)

Rathi, Mukesh Kumar; Singh, Tej; Chauhan, Ranchan

2018-05-01

In this research work, the dynamic mechanical properties of waste tyre rubber filled friction composites were studied. Four friction composites with varying amount of waste rubber (0, 4, 8, 12 wt.%) and barium sulphate (38, 42, 46, 50 wt.%) were designed and fabricated as per industrial norms. Dynamic mechanical analysis has been carried out to characterize the storage modulus, loss modulus and damping factor of the fabricated friction composite. Experimental results indicated that storage modulus decreases with increasing waste rubber content up to particular loading (4 wt.%), and after that it increases with further loading. The loss modulus of the composites increases steadily with increasing waste rubber content whereas, damping factor remain maximum for 12 wt.% waste rubber filled friction composites.

Modeling the interactions between compliant microcapsules and pillars in microchannels

NASA Astrophysics Data System (ADS)

Zhu, Guangdong; Alexeev, Alexander; Kumacheva, Eugenia; Balazs, Anna C.

2007-07-01

Using a computational model, we investigate the motion of microcapsules inside a microchannel that encompasses a narrow constriction. The microcapsules are composed of a compliant, elastic shell and an encapsulated fluid; these fluid-filled shells model synthetic polymeric microcapsules or biological cells (e.g., leukocytes). Driven by an imposed flow, the capsules are propelled along the microchannel and through the constricted region, which is formed by two pillars that lie in registry, extending from the top and bottom walls of the channels. The tops of these pillars (facing into the microchannel) are modified to exhibit either a neutral or an attractive interaction with the microcapsules. The pillars (and constriction) model topological features that can be introduced into microfluidic devices or the physical and chemical heterogeneities that are inherently present in biological vessels. To simulate the behavior of this complex system, we employ a hybrid method that integrates the lattice Boltzmann model (LBM) for fluid dynamics and the lattice spring model (LSM) for the micromechanics of elastic solids. Through this LBM/LSM technique, we probe how the capsule's stiffness and interaction with the pillars affect its passage through the chambers. The results yield guidelines for regulating the movement of microcarriers in microfluidic systems and provide insight into the flow properties of biological cells in capillaries.

MicroCT angiography detects vascular formation and regression in skin wound healing.

PubMed

Urao, Norifumi; Okonkwo, Uzoagu A; Fang, Milie M; Zhuang, Zhen W; Koh, Timothy J; DiPietro, Luisa A

2016-07-01

Properly regulated angiogenesis and arteriogenesis are essential for effective wound healing. Tissue injury induces robust new vessel formation and subsequent vessel maturation, which involves vessel regression and remodeling. Although formation of functional vasculature is essential for healing, alterations in vascular structure over the time course of skin wound healing are not well understood. Here, using high-resolution ex vivo X-ray micro-computed tomography (microCT), we describe the vascular network during healing of skin excisional wounds with highly detailed three-dimensional (3D) reconstructed images and associated quantitative analysis. We found that relative vessel volume, surface area and branching number are significantly decreased in wounds from day 7 to days 14 and 21. Segmentation and skeletonization analysis of selected branches from high-resolution images as small as 2.5μm voxel size show that branching orders are decreased in the wound vessels during healing. In histological analysis, we found that the contrast agent fills mainly arterioles, but not small capillaries nor large veins. In summary, high-resolution microCT revealed dynamic alterations of vessel structures during wound healing. This technique may be useful as a key tool in the study of the formation and regression of wound vessels. Copyright © 2016 Elsevier Inc. All rights reserved.

Orthostatic stress is necessary to maintain the dynamic range of cardiovascular control in space

NASA Technical Reports Server (NTRS)

Baisch, J. F.; Wolfram, G.; Beck, L.; Drummer, C.; Stormer, I.; Buckey, J.; Blomqvist, G.

2000-01-01

In the upright position, gravity fills the low-pressure systems of human circulation with blood and interstitial fluid in the sections below the diaphragm. Without gravity one pressure component in the vessels disappears and the relationship between hydrostatic pressure and oncotic pressure, which regulates fluid passage across the capillary endothelium in the terminal vascular bed, shifts constantly. The visible consequences of this are a puffy face and "bird" legs. The plasma volume shrinks in space and the range of cardiovascular control is reduced. When they stand up for the first time after landing, 30-50% of astronauts suffer from orthostatic intolerance. It remains unclear whether microgravity impairs cardiovascular reflexes, or whether it is the altered volume status that causes the cardiovascular instability following space flight. Lower body negative pressure was used in several space missions to stimulate the cardiovascular reflexes before, during and after a space flight. The results show that cardiovascular reflexes are maintained in microgravity. However, the astronauts' volume status changed in space, towards a volume-retracted state, as measurements of fluid-regulating hormones have shown. It can be hypothesized that the control of circulation and body fluid homeostasis in humans is adapted to their upright posture in the Earth's gravitational field. Autonomic control regulates fluid distribution to maintain the blood pressure in that posture, which most of us have to cope with for two-thirds of the day. A determined amount of interstitial volume is necessary to maintain the dynamic range of cardiovascular control in the upright posture; otherwise orthostatic intolerance may occur more often.

3D printed Lego®-like modular microfluidic devices based on capillary driving.

PubMed

Nie, Jing; Gao, Qing; Qiu, Jing-Jiang; Sun, Miao; Liu, An; Shao, Lei; Fu, Jian-Zhong; Zhao, Peng; He, Yong

2018-03-12

The field of how to rapidly assemble microfluidics with modular components continuously attracts researchers' attention, however, extra efforts must be devoted to solving the problems of leaking and aligning between individual modules. This paper presents a novel type of modular microfluidic device, driven by capillary force. There is no necessity for a strict seal or special alignment, and its open structures make it easy to integrate various stents and reactants. The key rationale for this method is to print different functional modules with a low-cost three-dimensional (3D) printer, then fill the channels with capillary materials and assemble them with plugs like Lego ® bricks. This rapidly reconstructed modular microfluidic device consists of a variety of common functional modules and other personalized modules, each module having a unified standard interface for easy assembly. As it can be printed by a desktop 3D printer, the manufacturing process is simple and efficient, with controllable regulation of the flow channel scale. Through diverse combinations of different modules, a variety of different functions can be achieved, without duplicating the manufacturing process. A single module can also be taken out for testing and analysis. What's more, combined with basic circuit components, it can serve as a low-cost Lego ® -like modular microfluidic circuits. As a proof of concept, the modular microfluidic device has been successfully demonstrated and used for stent degradation and cell cultures, revealing the potential use of this method in both chemical and biological research.

Automatic detection of recoil-proton tracks and background rejection criteria in liquid scintillator-micro-capillary-array fast neutron spectrometer

NASA Astrophysics Data System (ADS)

Mor, Ilan; Vartsky, David; Dangendorf, Volker; Tittelmeier, Kai.; Weierganz, Mathias; Goldberg, Mark Benjamin; Bar, Doron; Brandis, Michal

2018-06-01

We describe an analysis procedure for automatic unambiguous detection of fast-neutron-induced recoil proton tracks in a micro-capillary array filled with organic liquid scintillator. The detector is viewed by an intensified CCD camera. This imaging neutron detector possesses the capability to perform high position-resolution (few tens of μm), energy-dispersive transmission-imaging using ns-pulsed beams. However, when operated with CW or DC beams, it also features medium-quality spectroscopic capabilities for incident neutrons in the energy range 2-20 MeV. In addition to the recoil proton events which display a continuous extended track structure, the raw images exhibit complex ion-tracks from nuclear interactions of fast-neutrons in the scintillator, capillaries quartz-matrix and CCD. Moreover, as expected, one also observes a multitude of isolated scintillation spots of varying intensity (henceforth denoted "blobs") that originate from several different sources, such as: fragmented proton tracks, gamma-rays, heavy-ion reactions as well as events and noise that occur in the image-intensifier and CCD. In order to identify the continuous-track recoil proton events and distinguish them from all these background events, a rapid, computerized and automatic track-recognition-procedure was developed. Based on an appropriately weighted analysis of track parameters such as: length, width, area and overall light intensity, the method is capable of distinguishing a single continuous-track recoil proton from typically surrounding several thousands of background events that are found in each CCD frame.

Self-similarity and scaling transitions during rupture of thin free films of Newtonian fluids

NASA Astrophysics Data System (ADS)

Thete, Sumeet Suresh; Anthony, Christopher; Doshi, Pankaj; Harris, Michael T.; Basaran, Osman A.

2016-09-01

Rupture of thin liquid films is crucial in many industrial applications and nature such as foam stability in oil-gas separation units, coating flows, polymer processing, and tear films in the eye. In some of these situations, a liquid film may have two free surfaces (referred to here as a free film or a sheet) as opposed to a film deposited on a solid substrate that has one free surface. The rupture of such a free film or a sheet of a Newtonian fluid is analyzed under the competing influences of inertia, viscous stress, van der Waals pressure, and capillary pressure by solving a system of spatially one-dimensional evolution equations for film thickness and lateral velocity. The dynamics close to the space-time singularity where the film ruptures is asymptotically self-similar and, therefore, the problem is also analyzed by reducing the transient partial differential evolution equations to a corresponding set of ordinary differential equations in similarity space. For sheets with negligible inertia, it is shown that the dominant balance of forces involves solely viscous and van der Waals forces, with capillary force remaining negligible throughout the thinning process in a viscous regime. On the other hand, for a sheet of an inviscid fluid for which the effect of viscosity is negligible, it is shown that the dominant balance of forces is between inertial, capillary, and van der Waals forces as the film evolves towards rupture in an inertial regime. Real fluids, however, have finite viscosity. Hence, for real fluids, it is further shown that the viscous and the inertial regimes are only transitory and can only describe the initial thinning dynamics of highly viscous and slightly viscous sheets, respectively. Moreover, regardless of the fluid's viscosity, it is shown that for sheets that initially thin in either of these two regimes, their dynamics transition to a late stage or final inertial-viscous regime in which inertial, viscous, and van der Waals forces balance each other while capillary force remains negligible, in accordance with the results of Vaynblat, Lister, and Witelski.

Capillary droplet propulsion on a fibre.

PubMed

Haefner, Sabrina; Bäumchen, Oliver; Jacobs, Karin

2015-09-21

A viscous liquid film coating a fibre becomes unstable and decays into droplets due to the Rayleigh-Plateau instability (RPI). Here, we report on the generation of uniform droplets on a hydrophobized fibre by taking advantage of this effect. In the late stages of liquid column breakup, a three-phase contact line can be formed at one side of the droplet by spontaneous rupture of the thinning film. The resulting capillary imbalance leads to droplet propulsion along the fibre. We study the dynamics and the dewetting speed of the droplet as a function of molecular weight as well as temperature and compare to a force balance model based on purely viscous dissipation.

Dynamics of a grain-filled ball on a vibrating plate.

PubMed

Pacheco-Vázquez, F; Ludewig, F; Dorbolo, S

2014-09-12

We study experimentally how the bouncing dynamics of a hollow ball on a vibrating plate is modified when it is partially filled with liquid or grains. Whereas empty and liquid-filled balls display a dominant chaotic dynamics, a ball with grains exhibits a rich variety of stationary states, determined by the grain size and filling volume. In the collisional regime, i.e., when the energy injected to the system is mainly dissipated by interparticle collisions, an unexpected period-1 orbit appears independently of the vibration conditions, over a wide range. This is a self-regulated state driven by the formation and collapse of a granular gas within the ball during one cycle. In the frictional regime (dissipation dominated by friction), the grains move collectively and generate different patterns and steady modes: oscillons, waves, period doubling, etc. From a phase diagram and a geometrical analysis, we deduce that these modes are the result of a coupling (synchronization) between the vibrating plate frequency and the trajectory followed by the particles inside the cavity.

Dynamics of a Grain-Filled Ball on a Vibrating Plate

NASA Astrophysics Data System (ADS)

Pacheco-Vázquez, F.; Ludewig, F.; Dorbolo, S.

2014-09-01

We study experimentally how the bouncing dynamics of a hollow ball on a vibrating plate is modified when it is partially filled with liquid or grains. Whereas empty and liquid-filled balls display a dominant chaotic dynamics, a ball with grains exhibits a rich variety of stationary states, determined by the grain size and filling volume. In the collisional regime, i.e., when the energy injected to the system is mainly dissipated by interparticle collisions, an unexpected period-1 orbit appears independently of the vibration conditions, over a wide range. This is a self-regulated state driven by the formation and collapse of a granular gas within the ball during one cycle. In the frictional regime (dissipation dominated by friction), the grains move collectively and generate different patterns and steady modes: oscillons, waves, period doubling, etc. From a phase diagram and a geometrical analysis, we deduce that these modes are the result of a coupling (synchronization) between the vibrating plate frequency and the trajectory followed by the particles inside the cavity.

Heat Transfer by Thermo-capillary Convection -Sounding Rocket COMPERE Experiment SOURCE

NASA Astrophysics Data System (ADS)

Dreyer, Michael; Fuhrmann, Eckart

The sounding rocket COMPERE experiment SOURCE was successfully flown on MASER 11, launched in Kiruna (ESRANGE), May 15th, 2008. SOURCE has been intended to partly ful-fill the scientific objectives of the European Space Agency (ESA) Microgravity Applications Program (MAP) project AO-2004-111 (Convective boiling and condensation). Three parties of principle investigators have been involved to design the experiment set-up: ZARM for thermo-capillary flows, IMFT (Toulouse, France) for boiling studies, EADS Astrium (Bremen, Ger-many) for depressurization. The topic of this paper is to study the effect of wall heat flux on the contact line of the free liquid surface and to obtain a correlation for a convective heat trans-fer coefficient. The experiment has been conducted along a predefined time line. A preheating sequence at ground was the first operation to achieve a well defined temperature evolution within the test cell and its environment inside the rocket. Nearly one minute after launch, the pressurized test cell was filled with the test liquid HFE-7000 until a certain fill level was reached. Then the free surface could be observed for 120 s without distortion. Afterwards, the first depressurization was started to induce subcooled boiling, the second one to start saturated boiling. The data from the flight consists of video images and temperature measurements in the liquid, the solid, and the gaseous phase. Data analysis provides the surface shape versus time and the corresponding apparent contact angle. Computational analysis provides information for the determination of the heat transfer coefficient in a compensated gravity environment where a flow is caused by the temperature difference between the hot wall and the cold liquid. The paper will deliver correlations for the effective contact angle and the heat transfer coefficient as a function of the relevant dimensionsless parameters as well as physical explanations for the observed behavior. The data will be used for benchmarking of commercial CFD codes and the tank design (using these codes) of future cryogenic upper stages. References Eckart Fuhrmann, Michael E. Dreyer, Description of the Sounding Rocket Experiment SOURCE, Microgravity sci. technol., 20/3-4, 206 (2008)

High throughput nanoimprint lithography for semiconductor memory applications

NASA Astrophysics Data System (ADS)

Ye, Zhengmao; Zhang, Wei; Khusnatdinov, Niyaz; Stachowiak, Tim; Irving, J. W.; Longsine, Whitney; Traub, Matthew; Fletcher, Brian; Liu, Weijun

2017-03-01

Imprint lithography is a promising technology for replication of nano-scale features. For semiconductor device applications, Canon deposits a low viscosity resist on a field by field basis using jetting technology. A patterned mask is lowered into the resist fluid which then quickly flows into the relief patterns in the mask by capillary action. Following this filling step, the resist is crosslinked under UV radiation, and then the mask is removed, leaving a patterned resist on the substrate. There are two critical components to meeting throughput requirements for imprint lithography. Using a similar approach to what is already done for many deposition and etch processes, imprint stations can be clustered to enhance throughput. The FPA-1200NZ2C is a four station cluster system designed for high volume manufacturing. For a single station, throughput includes overhead, resist dispense, resist fill time (or spread time), exposure and separation. Resist exposure time and mask/wafer separation are well understood processing steps with typical durations on the order of 0.10 to 0.20 seconds. To achieve a total process throughput of 17 wafers per hour (wph) for a single station, it is necessary to complete the fluid fill step in 1.2 seconds. For a throughput of 20 wph, fill time must be reduced to only one 1.1 seconds. There are several parameters that can impact resist filling. Key parameters include resist drop volume (smaller is better), system controls (which address drop spreading after jetting), Design for Imprint or DFI (to accelerate drop spreading) and material engineering (to promote wetting between the resist and underlying adhesion layer). In addition, it is mandatory to maintain fast filling, even for edge field imprinting. In this paper, we address the improvements made in all of these parameters to first enable a 1.20 second filling process for a device like pattern and have demonstrated this capability for both full fields and edge fields. Non-fill defectivity is well under 1.0 defects/cm2 for both field types. Next, by further reducing drop volume and optimizing drop patterns, a fill time of 1.1 seconds was demonstrated.

Evaporation from bare ground with different water-table depths based on an in-situ experiment in Ordos Plateau, China

NASA Astrophysics Data System (ADS)

Zhang, Zaiyong; Wang, Wenke; Wang, Zhoufeng; Chen, Li; Gong, Chengcheng

2018-03-01

The dynamic processes of ground evaporation are complex and are related to a multitude of factors such as meteorological influences, water-table depth, and materials in the unsaturated zone. To investigate ground evaporation from a homogeneous unsaturated zone, an in-situ experiment was conducted in Ordos Plateau of China. Two water-table depths were chosen to explore the water movement in the unsaturated zone and ground evaporation. Based on the experimental and calculated results, it was revealed that (1) bare ground evaporation is an atmospheric-limited stage for the case of water-table depth being close to the capillary height; (2) the bare ground evaporation is a water-storage-limited stage for the case of water-table depth being beyond the capillary height; (3) groundwater has little effect on ground-surface evaporation when the water depth is larger than the capillary height; and (4) ground evaporation is greater at nighttime than that during the daytime; and (5) a liquid-vapor interaction zone at nearly 20 cm depth is found, in which there exists a downward vapor flux on sunny days, leading to an increasing trend of soil moisture between 09:00 to 17:00; the maximum value is reached at midday. The results of this investigation are useful to further understand the dynamic processes of ground evaporation in arid areas.

Optimal MEMS device for mobility and zeta potential measurements using DC electrophoresis.

PubMed

Karam, Pascal R; Dukhin, Andrei; Pennathur, Sumita

2017-05-01

We have developed a novel microchannel geometry that allows us to perform simple DC electrophoresis to measure the electrophoretic mobility and zeta potential of analytes and particles. In standard capillary geometries, mobility measurements using DC fields are difficult to perform. Specifically, measurements in open capillaries require knowledge of the hard to measure and often dynamic wall surface potential. Although measurements in closed capillaries eliminate this requirement, the measurements must be performed at infinitesimally small regions of zero flow where the pressure driven-flow completely cancels the electroosmotic flow (Komagata Planes). Furthermore, applied DC fields lead to electrode polarization, further questioning the reliability and accuracy of the measurement. In contrast, our geometry expands and moves the Komagata planes to where velocity gradients are at a minimum, and thus knowledge of the precise location of a Komagata plane is not necessary. Additionally, our microfluidic device prevents electrode polarization because of fluid recirculation around the electrodes. We fabricated our device using standard MEMS fabrication techniques and performed electrophoretic mobility measurements on 500 nm fluorescently tagged polystyrene particles at various buffer concentrations. Results are comparable to two different commercial dynamic light scattering based particle sizing instruments. We conclude with guidelines to further develop this robust electrophoretic tool that allows for facile and efficient particle characterization. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Design and Operation of a Cryogenic Nitrogen Pulsating Heat Pipe

NASA Astrophysics Data System (ADS)

Diego Fonseca, Luis; Miller, Franklin; Pfotenhauer, John

2015-12-01

We report the design, experimental setup and successful test results using an innovative passive cooling system called a “Pulsating Heat Pipe” (PHP) operating at temperatures ranging from 77 K to 80 K and using nitrogen as the working fluid. PHPs, which transfer heat by two phase flow mechanisms through a closed loop tubing have the advantage that no electrical pumps are needed to drive the fluid flow. In addition, PHPs have an advantage over copper straps and thermal conductors since they are lighter in weight, exhibit lower temperature gradients and have higher heat transfer rates. PHPs consist of an evaporator section, thermally anchored to a solid, where heat is received at the saturation temperature where the liquid portion of the two-phase flow evaporates, and a condenser where heat is rejected at the saturation temperature where the vapor is condensed. The condenser section in our experiment has been thermally interfaced to a CT cryocooler from SunPower that has a cooling capacity of 10 W at 77 K. Alternating regions of liquid slugs and small vapor plugs fill the capillary tubing, with the vapor regions contracting in the condenser section and expanding in the evaporator section due to an electric heater that will generate heat loads up to 10 W. This volumetric expansion and contraction provides the oscillatory flow of the fluid throughout the capillary tubing thereby transferring heat from one end to the other. The thermal performance and temperature characteristics of the PHP will be correlated as a function of average condenser temperature, PHP fill liquid ratio, and evaporator heat load. The experimental data show that the heat transfer between the evaporator and condenser sections can produce an effective thermal conductivity up to 35000 W/m-K at a 3.5 W heat load.

Influence of laser wavelength and pulse duration on gas bubble formation in blood filled glass capillaries.

PubMed

Kimel, Sol; Choi, Bernard; Svaasand, Lars O; Lotfi, Justin; Viator, John A; Nelson, J Stuart

2005-04-01

Hypervascular skin lesions (HVSL) are treated with medical lasers characterized by a variety of parameters such as wavelength lambda, pulse duration t(p), and radiant exposure E that can be adjusted for different pathology and blood vessel size. Treatment parameters have been optimized assuming constant optical properties of blood during laser photocoagulation. However, recent studies suggest that this assumption may not always be true. Our objective was to quantify thermally induced changes in blood that occur during irradiation using standard laser parameters. Glass capillary tubes (diameter D = 100, 200, and 337 microm) filled with fresh or hemolyzed rabbit blood were irradiated once at lambda = 585, 595, or 600 nm, t(p) = 1.5 milliseconds; and also at lambda = 585 nm, t(p) = 0.45 milliseconds. E was increased until blood ablation caused formation of permanent gas bubbles. In a corroborative study, human blood was heated at 50 degrees C and absorbance spectra were measured as a function of time. Threshold radiant exposure, E(thresh), for gas bubble formation was found not to depend on lambda, which might be surprising in view of the 10-fold lower absorption coefficient at 600 nm as compared to 585 nm. The spectroscopic study revealed heat-induced changes in blood constituent composition of hemoglobins (Hb) from initially 100% oxyhemoglobin (HbO2) to deoxyhemoglobin (HHb) and, ultimately, methemoglobin (metHb) as the major constituent. Model calculations of E(thresh)(lambda,D) based on changing constituent blood composition during heating with milliseconds lasers were found to correlate with experimental results. For laser treatment of HVSL it appears that lambda is of secondary importance and that the choice of t(p) is a more important factor. Copyright 2005 Wiley-Liss, Inc.

Noninvasive estimation of tissue edema in healthy volunteers and in patients suffering from heart failure

NASA Astrophysics Data System (ADS)

Gurfinkel, Yuri I.; Mikhailov, Valery M.; Kudutkina, Marina I.

2004-06-01

Capillaries play a critical role in cardiovascular function as the point of exchange of nutrients and waste products between tissues and circulation. A common problem for healthy volunteers examined during isolation, and for the patients suffering from heart failure is a quantitative estimation tissue oedema. Until now, objective assessment body fluids retention in tissues did not exist. Optical imaging of living capillaries is a challenging and medically important scientific problem. Goal of the investigation was to study dynamic of microcriculation parameters including tissue oedema in healthy volunteers during extended isolation and relative hypokinesia as a model of mission to the International Space Station. The other aim was to study dynamic of microcirculation parameters including tissue oedema in patients suffering from heart failure under treatment. Healthy volunteers and patients. We studied four healthy male subjects at the age of 41, 37, 40, and 48 before the experiment (June 1999), and during the 240-d isolation period starting from July3, 1999. Unique hermetic chambers with artidicial environmental parameters allowed performing this study with maximum similarity to real conditions in the International Space Station (ISS). With the regularity of 3 times a week at the same time, each subject recorded three video episodes with the total length of one-minute using the optical computerized capillaroscope for noninvasive measurement of the capillary diameters sizes, capillary blood velocity as well as the size of the perivascular zone. All this parameters of microcirculation determined during three weeks in 15 patients (10 male, 5 female, aged 62,2+/-8,8) suffering from heart failure under Furosemid 40 mg 2 times a week, as diuretic. Results. About 1500 episodes recorded on laser disks and analyzed during this experiment. Every subject had wave-like variations of capillary blood velocity within the minute, week, and month ranges. It was found that the perivascular zone sizes rising during isolation correlate with body mass of subjects and probably depend on retention of body fluids in tissues. Computerized capillaroscopy provides a new opportunity for non-invasive quantitative estimation tissue oedema and suggests for exact management patients suffering from heart failure under diuretic treatment.

Effect of wettability on two-phase quasi-static displacement: Validation of two pore scale modeling approaches

NASA Astrophysics Data System (ADS)

Verma, Rahul; Icardi, Matteo; Prodanović, Maša

2018-05-01

Understanding of pore-scale physics for multiphase flow in porous media is essential for accurate description of various flow phenomena. In particular, capillarity and wettability strongly influence capillary pressure-saturation and relative permeability relationships. Wettability is quantified by the contact angle of the fluid-fluid interface at the pore walls. In this work we focus on the non-trivial interface equilibria in presence of non-neutral wetting and complex geometries. We quantify the accuracy of a volume-of-fluid (VOF) formulation, implemented in a popular open-source computational fluid dynamics code, compared with a new formulation of a level set (LS) method, specifically developed for quasi-static capillarity-dominated displacement. The methods are tested in rhomboidal packings of spheres for a range of contact angles and for different rhomboidal configurations and the accuracy is evaluated against the semi-analytical solutions obtained by Mason and Morrow (1994). While the VOF method is implemented in a general purpose code that solves the full Navier-Stokes (NS) dynamics in a finite volume formulation, with additional terms to model surface tension, the LS method is optimized for the quasi-static case and, therefore, less computationally expensive. To overcome the shortcomings of the finite volume NS-VOF system for low capillary number flows, and its computational cost, we introduce an overdamped dynamics and a local time stepping to speed up the convergence to the steady state, for every given imposed pressure gradient (and therefore saturation condition). Despite these modifications, the methods fundamentally differ in the way they capture the interface, as well as in the number of equations solved and in the way the mean curvature (or equivalently capillary pressure) is computed. This study is intended to provide a rigorous validation study and gives important indications on the errors committed by these methods in solving more complex geometry and dynamics, where usually many sources of errors are interplaying.

Interwoven four-compartment capillary membrane technology for three-dimensional perfusion with decentralized mass exchange to scale up embryonic stem cell culture.

PubMed

Gerlach, Jörg C; Lübberstedt, Marc; Edsbagge, Josefina; Ring, Alexander; Hout, Mariah; Baun, Matt; Rossberg, Ingrid; Knöspel, Fanny; Peters, Grant; Eckert, Klaus; Wulf-Goldenberg, Annika; Björquist, Petter; Stachelscheid, Harald; Urbaniak, Thomas; Schatten, Gerald; Miki, Toshio; Schmelzer, Eva; Zeilinger, Katrin

2010-01-01

We describe hollow fiber-based three-dimensional (3D) dynamic perfusion bioreactor technology for embryonic stem cells (ESC) which is scalable for laboratory and potentially clinical translation applications. We added 2 more compartments to the typical 2-compartment devices, namely an additional media capillary compartment for countercurrent 'arteriovenous' flow and an oxygenation capillary compartment. Each capillary membrane compartment can be perfused independently. Interweaving the 3 capillary systems to form repetitive units allows bioreactor scalability by multiplying the capillary units and provides decentralized media perfusion while enhancing mass exchange and reducing gradient distances from decimeters to more physiologic lengths of <1 mm. The exterior of the resulting membrane network, the cell compartment, is used as a physically active scaffold for cell aggregation; adjusting intercapillary distances enables control of the size of cell aggregates. To demonstrate the technology, mouse ESC (mESC) were cultured in 8- or 800-ml cell compartment bioreactors. We were able to confirm the hypothesis that this bioreactor enables mESC expansion qualitatively comparable to that obtained with Petri dishes, but on a larger scale. To test this, we compared the growth of 129/SVEV mESC in static two-dimensional Petri dishes with that in 3D perfusion bioreactors. We then tested the feasibility of scaling up the culture. In an 800-ml prototype, we cultured approximately 5 x 10(9) cells, replacing up to 800 conventional 100-mm Petri dishes. Teratoma formation studies in mice confirmed protein expression and gene expression results with regard to maintaining 'stemness' markers during cell expansion. Copyright 2010 S. Karger AG, Basel.

Phenotypic heterogeneity in lung capillary and extra-alveolar endothelial cells. Increased extra-alveolar endothelial permeability is sufficient to decrease compliance.

PubMed

Lowe, Kevin; Alvarez, Diego; King, Judy; Stevens, Troy

2007-11-01

In acute respiratory distress syndrome, pulmonary vascular permeability increases, causing intravascular fluid and protein to move into the lung's interstitium. The classic model describing the formation of pulmonary edema suggests that fluid crossing the capillary endothelium is drawn by negative interstitial pressure into the potential space surrounding extra-alveolar vessels and, as interstitial pressure builds, is forced into the alveolar air space. However, the validity of this model is challenged by animal models of acute lung injury in which extra-alveolar vessels are more permeable than capillaries under a variety of conditions. In the current study, we sought to determine whether extravascular fluid accumulation can be produced because of increased permeability of either the capillary or extra-alveolar endothelium, and whether different pathophysiology results from such site-specific increases in permeability. We perfused isolated lungs with either the plant alkaloid thapsigargin, which increases extra-alveolar endothelial permeability, or with 4alpha-phorbol 12, 13-didecanoate, which increases capillary endothelial permeability. Both treatments produced equal increases in whole lung vascular permeability, but caused fluid accumulations in separate anatomical compartments. Light microscopy of isolated lungs showed that thapsigargin caused fluid cuffing of large vessels, while 4alpha-phorbol 12, 13-didecanoate caused alveolar flooding. Dynamic compliance was reduced in lungs with cuffing of large vessels, but not in lungs with alveolar flooding. Phenotypic differences between vascular segments resulted in site-specific increases in permeability, which have different pathophysiological outcomes. Our findings suggest that insults leading to acute respiratory distress syndrome may increase permeability in extra-alveolar or capillary vascular segments, resulting in different pathophysiological sequela.

Using Neutron Radiography to Quantify Water Transport and the Degree of Saturation in Entrained Air Cement Based Mortar

NASA Astrophysics Data System (ADS)

Lucero, Catherine L.; Bentz, Dale P.; Hussey, Daniel S.; Jacobson, David L.; Weiss, W. Jason

Air entrainment is commonly added to concrete to help in reducing the potential for freeze thaw damage. It is hypothesized that the entrained air voids remain unsaturated or partially saturated long after the smaller pores fill with water. Small gel and capillary pores in the cement matrix fill quickly on exposure to water, but larger pores (entrapped and entrained air voids) require longer times or other methods to achieve saturation. As such, it is important to quantitatively determine the water content and degree of saturation in air entrained cementitious materials. In order to further investigate properties of cement-based mortar, a model based on Beer's Law has been developed to interpret neutron radiographs. This model is a powerful tool for analyzing images acquired from neutron radiography. A mortar with a known volume of aggregate, water to cement ratio and degree of hydration can be imaged and the degree of saturation can be estimated.

Drop deformation and breakup in a partially filled horizontal rotating cylinder

NASA Astrophysics Data System (ADS)

White, Andrew; Pereira, Caroline; Hyacinthe, Hyaquino; Ward, Thomas

2014-11-01

Drop deformation and breakup due to shear flow has been studied extensively in Couette devices as well as in gravity-driven flows. In these cases shear is generated either by the moving wall or the drop's motion. For such flows the drop shape remains unperturbed at low capillary number (Ca), deforms at moderate Ca , and can experience breakup as Ca --> 1 and larger. Here single drops of NaOH(aq) will be placed in a horizontal cylindrical rotating tank partially filled with vegetable oil resulting in 10-2 < Ca <101 . It will be shown that the reactive vegetable oil-NaOH(aq) system, where surfactants are produced in situ by saponification, can yield lower minimum surface tensions and faster adsorption than non-reactive surfactant systems. Oil films between the wall and drop as well as drop shape will be observed as rotation rates and NaOH(aq) concentration are varied. Results will be presented in the context of previous work on bubble and drop shapes and breakup. NSF CBET #1262718.

Experimental analysis for fabrication of high-aspect-ratio piezoelectric ceramic structure by micro-powder injection molding process

NASA Astrophysics Data System (ADS)

Han, Jun Sae; Gal, Chang Woo; Park, Jae Man; Kim, Jong Hyun; Park, Seong Jin

2018-04-01

Aspect ratio effects in the micro-powder injection molding process were experimentally analyzed for fabrication of high-aspect-ratio piezoelectric ceramic structure. The mechanisms of critical defects have been studied according to individual manufacturing steps. In the molding process, incomplete filling phenomenon determines the critical aspect ratios of a micro pattern. According to mold temperature, an incomplete filling phenomenon has been analyzed with respect to different pattern sizes and aspect ratio. In demolding and drying process, the capillary behavior of sacrificial polymeric mold insert determines the critical aspect ratio of a micro pattern. With respect to pattern dimensions, slumping behavior has been analyzed. Based on our current systems, micro PZT feature has stability when it has lower aspect ratio than 5. Under optimized processing conditions, 20 μm and 40 μm ceramic rod array feature which has 5 of aspect ratio were successfully fabricated by the developed process. Further modification points to fabricate the smaller and higher feature were specifically addressed.

Determination of blood oxygenation in the brain by time-resolved reflectance spectroscopy: influence of the skin, skull, and meninges

NASA Astrophysics Data System (ADS)

Hielscher, Andreas H.; Liu, Hanli; Wang, Lihong V.; Tittel, Frank K.; Chance, Britton; Jacques, Steven L.

1994-07-01

Near infrared light has been used for the determination of blood oxygenation in the brain but little attention has been paid to the fact that the states of blood oxygenation in arteries, veins, and capillaries differ substantially. In this study, Monte Carlo simulations for a heterogeneous system were conducted, and near infrared time-resolved reflectance measurements were performed on a heterogeneous tissue phantom model. The model was made of a solid polyester resin, which simulates the tissue background. A network of tubes was distributed uniformly through the resin to simulate the blood vessels. The time-resolved reflectance spectra were taken with different absorbing solutions filled in the network. Based on the simulation and experimental results, we investigated the dependence of the absorption coefficient obtained from the heterogeneous system on the absorption of the actual absorbing solution filled in the tubes. We show that light absorption by the brain should result from the combination of blood and blood-free tissue background.

Development of novel separation techniques for biological samples in capillary electrophoresis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chang, Huan -Tsung

1994-07-27

This dissertation includes three different topics: general introduction of capillary electrophoresis (CE); gradient in CE and CE in biological separations; and capillary gel electrophoresis (CGE) for DNA separation. Factors such as temperature, viscosity, pH, and the surface of capillary walls affecting the separation performance are demonstrated. A pH gradient between 3.0 and 5.2 is useful to improve the resolution among eight different organic acids. A flow gradient due to the change in the concentration of surfactant, which is able to coat to the capillary wall to change the flow rate and its direction, is also shown as a good waymore » to improve the resolution for organic compounds. A temperature gradient caused by joule heat is shown by voltage programming to enhance the resolution and shorten the separation time for several phenolic compounds. The author also shows that self-regulating dynamic control of electroosmotic flow in CE by simply running separation in different concentrations of surfactant has less matrix effect on the separation performance. One of the most important demonstrations in this dissertation is that the author proposes on-column reaction which gives several advantages including the use of a small amount of sample, low risk of contamination, and time saving and kinetic features. The author uses this idea with laser induced fluorescence (LIF) as a detection mode to detect an on-column digestion of sub-ng of protein. This technique also is applied to single cell analysis in the group.« less

Blood Vessel Adaptation with Fluctuations in Capillary Flow Distribution

PubMed Central

Hu, Dan; Cai, David; Rangan, Aaditya V.

2012-01-01

Throughout the life of animals and human beings, blood vessel systems are continuously adapting their structures – the diameter of vessel lumina, the thickness of vessel walls, and the number of micro-vessels – to meet the changing metabolic demand of the tissue. The competition between an ever decreasing tendency of luminal diameters and an increasing stimulus from the wall shear stress plays a key role in the adaptation of luminal diameters. However, it has been shown in previous studies that the adaptation dynamics based only on these two effects is unstable. In this work, we propose a minimal adaptation model of vessel luminal diameters, in which we take into account the effects of metabolic flow regulation in addition to wall shear stresses and the decreasing tendency of luminal diameters. In particular, we study the role, in the adaptation process, of fluctuations in capillary flow distribution which is an important means of metabolic flow regulation. The fluctuation in the flow of a capillary group is idealized as a switch between two states, i.e., an open-state and a close-state. Using this model, we show that the adaptation of blood vessel system driven by wall shear stress can be efficiently stabilized when the open time ratio responds sensitively to capillary flows. As micro-vessel rarefaction is observed in our simulations with a uniformly decreased open time ratio of capillary flows, our results point to a possible origin of micro-vessel rarefaction, which is believed to induce hypertension. PMID:23029014

Substructured multibody molecular dynamics.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grest, Gary Stephen; Stevens, Mark Jackson; Plimpton, Steven James

2006-11-01

We have enhanced our parallel molecular dynamics (MD) simulation software LAMMPS (Large-scale Atomic/Molecular Massively Parallel Simulator, lammps.sandia.gov) to include many new features for accelerated simulation including articulated rigid body dynamics via coupling to the Rensselaer Polytechnic Institute code POEMS (Parallelizable Open-source Efficient Multibody Software). We use new features of the LAMMPS software package to investigate rhodopsin photoisomerization, and water model surface tension and capillary waves at the vapor-liquid interface. Finally, we motivate the recipes of MD for practitioners and researchers in numerical analysis and computational mechanics.

Dynamic behaviors of liquid droplets on a gas diffusion layer surface: Hybrid lattice Boltzmann investigation

NASA Astrophysics Data System (ADS)

Wu, Jie; Huang, Jun-Jie

2015-07-01

Water management is one of the key issues in proton exchange membrane fuel cells. Fundamentally, it is related to dynamic behaviors of droplets on a gas diffusion layer (GDL) surface, and consequently they are investigated in this work. A two-dimensional hybrid method is employed to implement numerical simulations, in which the flow field is solved by using the lattice Boltzmann method and the interface between droplet and gas is captured by solving the Cahn-Hilliard equation directly. One or two liquid droplets are initially placed on the GDL surface of a gas channel, which is driven by the fully developed Poiseuille flow. At a fixed channel size, the effects of viscosity ratio of droplet to gas ( μ ∗ ), Capillary number (Ca, ratio of gas viscosity to surface tension), and droplet interaction on the dynamic behaviors of droplets are systematically studied. By decreasing viscosity ratio or increasing Capillary number, the single droplet can detach from the GDL surface easily. On the other hand, when two identical droplets stay close to each other or a larger droplet is placed in front of a smaller droplet, the removal of two droplets is promoted.

Dynamical phase separation using a microfluidic device: experiments and modeling

NASA Astrophysics Data System (ADS)

Aymard, Benjamin; Vaes, Urbain; Radhakrishnan, Anand; Pradas, Marc; Gavriilidis, Asterios; Kalliadasis, Serafim; Complex Multiscale Systems Team

2017-11-01

We study the dynamical phase separation of a binary fluid by a microfluidic device both from the experimental and from the modeling points of view. The experimental device consists of a main channel (600 μm wide) leading into an array of 276 trapezoidal capillaries of 5 μm width arranged on both sides and separating the lateral channels from the main channel. Due to geometrical effects as well as wetting properties of the substrate, and under well chosen pressure boundary conditions, a multiphase flow introduced into the main channel gets separated at the capillaries. Understanding this dynamics via modeling and numerical simulation is a crucial step in designing future efficient micro-separators. We propose a diffuse-interface model, based on the classical Cahn-Hilliard-Navier-Stokes system, with a new nonlinear mobility and new wetting boundary conditions. We also propose a novel numerical method using a finite-element approach, together with an adaptive mesh refinement strategy. The complex geometry is captured using the same computer-aided design files as the ones adopted in the fabrication of the actual device. Numerical simulations reveal a very good qualitative agreement between model and experiments, demonstrating also a clear separation of phases.

Unusual Contact-Line Dynamics of Thick Films and Drops

NASA Technical Reports Server (NTRS)

Veretennikov, Igor; Agarwal, Abhishek; Indeikina, Alexandra; Chang, Hsueh-Chia

1999-01-01

We report several novel phenomena In contact-line and fingering dynamics of macroscopic spinning drops and gravity-driven films with dimensions larger than the capillary length. It is shown through experimental and theoretical analysis that such macroscopic films can exhibit various interfacial shapes, including multi valued ones, near the contact line due to a balance between the external body forces with capillarity. This rich variety of front shapes couples with the usual capillary, viscous, and intermolecular forces at the contact line to produce a rich and unexpected spectrum of contact-line dynamics. A single finger develops when part of the front becomes multivalued on a partially wetting macroscopic spinning drop in contrast to a different mechanism for microscopic drops of completely wetting fluids. Contrary to general expectation, we observe that, at high viscosity and low frequencies of rotation, the speed of a glycerine finger increases with increasing viscosity. Completely wetting Dow Corning 200 Fluid spreads faster over a dry inclined plane than a prewetted one. The presence of a thin prewetted film suppresses fingering both for gravity-driven flow and for spin coating. We analyze some of these unique phenomena in detail and offer qualitative physical explanations for the others.

Radial fingering under arbitrary viscosity and density ratios

NASA Astrophysics Data System (ADS)

Anjos, Pedro H. A.; Dias, Eduardo O.; Miranda, José A.

2017-08-01

We study viscous fingering formation in radial Hele-Shaw cell geometry considering the combined action of capillary and inertial effects for arbitrary values of viscosity and density ratios. We tackle the problem by employing a perturbative mode-coupling approach and focus our attention on weakly nonlinear stages of the dynamics. If inertial effects are neglected, our theoretical results indicate that the shape of the resulting interfacial patterns is significantly affected by changes in the viscosity ratio. Under such conditions, the growing fingers tend to proliferate through a repeated ramification process (e.g., by finger bifurcation, quadrifurcation, etc.) as the capillary number is increased. Nevertheless, we find that this scenario is dramatically altered when inertia is taken into account. When inertia is relevant, the conventional finger splitting morphologies are replaced by three-lobed structures, characterized by the occurrence of sidebranching phenomena. We verify that slightly different types of sidebranched patterns arise, presenting either wide or sharp fingertips, for a range of capillary numbers and density ratios.

Capillary evaporation of the ionic liquid [EMIM][BF4] in nanoscale solvophobic confinement

NASA Astrophysics Data System (ADS)

Shrivastav, Gourav; Remsing, Richard C.; Kashyap, Hemant K.

2018-05-01

Solvent density fluctuations play a crucial role in liquid-vapor transitions in solvophobic confinement and can also be important for understanding solvation of polar and apolar solutes. In the case of ionic liquids (ILs), density fluctuations can be used to understand important processes in the context of nanoscale aggregation and colloidal self-assemblies. In this article, we explore the nature of density fluctuations associated with capillary evaporation of the IL 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIM][BF4]) in the confined region of model solvophobic nanoscale sheets by using molecular dynamics simulations combined with non-Boltzmann sampling techniques. We demonstrate that density fluctuations of the confined IL play an important role in capillary evaporation, suggesting analogies to dewetting transitions involving water. Significant changes in the interfacial structure of the IL are also detailed and suggested to underlie a non-classical (non-parabolic) dependence of the free energy barrier to evaporation on the degree of confinement.

Tube radial distribution phenomenon with a two-phase separation solution of a fluorocarbon and hydrocarbon organic solvent mixture in a capillary tube and metal compounds separation.

PubMed

Kitaguchi, Koichi; Hanamura, Naoya; Murata, Masaharu; Hashimoto, Masahiko; Tsukagoshi, Kazuhiko

2014-01-01

A fluorocarbon and hydrocarbon organic solvent mixture is known as a temperature-induced phase-separation solution. When a mixed solution of tetradecafluorohexane as a fluorocarbon organic solvent and hexane as a hydrocarbon organic solvent (e.g., 71:29 volume ratio) was delivered in a capillary tube that was controlled at 10°C, the tube radial distribution phenomenon (TRDP) of the solvents was clearly observed through fluorescence images of the dye, perylene, dissolved in the mixed solution. The homogeneous mixed solution (single phase) changed to a heterogeneous solution (two phases) with inner tetradecafluorohexane and outer hexane phases in the tube under laminar flow conditions, generating the dynamic liquid-liquid interface. We also tried to apply TRDP to a separation technique for metal compounds. A model analyte mixture, copper(II) and hematin, was separated through the capillary tube, and detected with a chemiluminescence detector in this order within 4 min.

Dynamics of associative polymer solutions: Capillary break-up, jetting and rheology

NASA Astrophysics Data System (ADS)

Sharma, Vivek; Serdy, James G.; Threfall-Holmes, Phil; McKinley, Gareth H.

2010-03-01

Associative polymer solutions are used in extensively in the formulations for water-borne paints, food, inks, cosmetics, etc to control the rheology and processing behavior of multi-component dispersions. The commercially relevant formulations use dilute solutions of associative polymers, which have low viscosity and short relaxation times, and hence their non-Newtonian response is not apparent in a conventional rheometer. In this talk, we explore several methods for systematically exploring the linear and nonlinear solution rheology of associative polymer dispersions, including: high frequency oscillatory tests at frequencies up to 10 kHz, microfluidic shear rheometry at deformation rates up to 10^6 s-1 and the influence of transientextensional rheology in the jet breakup. The presence of inertial, elastic and viscous effects typically leads to complex dynamics in a necking fluid thread. We show that by carefully controlling the excitation frequency, it is possible to drive the break-up in a particularly simple and symmetric mode, which can be used to extract extensional viscosity information using capillary thinning analysis.

Stabilizing detached Bridgman melt crystal growth: Proportional-integral feedback control

NASA Astrophysics Data System (ADS)

Yeckel, Andrew; Daoutidis, Prodromos; Derby, Jeffrey J.

2012-10-01

The dynamics, operability limits, and tuning of a proportional-integral feedback controller to stabilize detached vertical Bridgman crystal growth are analyzed using a capillary model of shape stability. The manipulated variable is the pressure difference between upper and lower vapor spaces, and the controlled variable is the gap width at the triple-phase line. Open and closed loop dynamics of step changes in these state variables are analyzed under both shape stable and shape unstable growth conditions. Effects of step changes in static contact angle and growth angle are also studied. Proportional and proportional-integral control can stabilize unstable growth, but only within tight operability limits imposed by the narrow range of allowed meniscus shapes. These limits are used to establish safe operating ranges of controller gain. Strong nonlinearity of the capillary model restricts the range of perturbations that can be stabilized, and under some circumstances, stabilizes a spurious operating state far from the set point. Stabilizing detachment at low growth angle proves difficult and becomes impossible at zero growth angle.

Determining tumor blood flow parameters from dynamic image measurements

NASA Astrophysics Data System (ADS)

Libertini, Jessica M.

2008-11-01

Many recent cancer treatments focus on preventing angiogenesis, the process by which a tumor promotes the growth of large and efficient capillary beds for the increased nourishment required to support the tumor's rapid growth[l]. To measure the efficacy of these treatments in a timely fashion, there is an interest in using data from dynamic sequences of contrast-enhanced medical imaging, such as MRI and CT, to measure blood flow parameters such as perfusion, permeability-surface-area product, and the relative volumes of the plasma and extracellular-extravascular space. Starting with a two compartment model presented by the radiology community[2], this work challenges the application of a simplification to this problem, which was originally developed to model capillary reuptake[3]. While the primary result of this work is the demonstration of the inaccuracy of this simplification, the remainder of the paper is dedicated to presenting alternative methods for calculating the perfusion and plasma volume coefficients. These methods are applied to model data sets based on real patient data, and preliminary results are presented.

Numerical modeling of interface displacement in heterogeneously wetting porous media

NASA Astrophysics Data System (ADS)

Hiller, T.; Brinkmann, M.; Herminghaus, S.

2013-12-01

We use the mesoscopic particle method stochastic rotation dynamics (SRD) to simulate immiscible multi-phase flow on the pore and sub-pore scale in three dimensions. As an extension to the standard SRD method, we present an approach on implementing complex wettability on heterogeneous surfaces. We use 3D SRD to simulate immiscible two-phase flow through a model porous medium (disordered packing of spherical beads) where the substrate exhibits different spatial wetting patterns. The simulations are designed to resemble experimental measurements of capillary pressure saturation. We show that the correlation length of the wetting patterns influences the temporal evolution of the interface and thus percolation, residual saturation and work dissipated during the fluid displacement. Our numerical results are in qualitatively good agreement with the experimental data. Besides of modeling flow in porous media, our SRD implementation allows us to address various questions of interfacial dynamics, e.g. the formation of capillary bridges between spherical beads or droplets in microfluidic applications to name only a few.

Computational fluid dynamics assisted characterization of parafoveal hemodynamics in normal and diabetic eyes using adaptive optics scanning laser ophthalmoscopy.

PubMed

Lu, Yang; Bernabeu, Miguel O; Lammer, Jan; Cai, Charles C; Jones, Martin L; Franco, Claudio A; Aiello, Lloyd Paul; Sun, Jennifer K

2016-12-01

Diabetic retinopathy (DR) is the leading cause of visual loss in working-age adults worldwide. Previous studies have found hemodynamic changes in the diabetic eyes, which precede clinically evident pathological alterations of the retinal microvasculature. There is a pressing need for new methods to allow greater understanding of these early hemodynamic changes that occur in DR. In this study, we propose a noninvasive method for the assessment of hemodynamics around the fovea (a region of the eye of paramount importance for vision). The proposed methodology combines adaptive optics scanning laser ophthalmoscopy and computational fluid dynamics modeling. We compare results obtained with this technique with in vivo measurements of blood flow based on blood cell aggregation tracking. Our results suggest that parafoveal hemodynamics, such as capillary velocity, wall shear stress, and capillary perfusion pressure can be noninvasively and reliably characterized with this method in both healthy and diabetic retinopathy patients.

Low-gravity fluid dynamics and transport phenomena. Progress in Astronautics and Aeronautics. Vol. 130

DOE Office of Scientific and Technical Information (OSTI.GOV)

Koster, J.N.; Sani, R.L.

1990-01-01

Various papers on low-gravity fluid dynamics and transport phenomena are presented. Individual topics addressed include: fluid management in low gravity, nucleate pool boiling in variable gravity, application of energy-stability theory to problems in crystal growth, thermosolutal convection in liquid HgCdTe near the liquidus temperature, capillary surfaces in microgravity, thermohydrodynamic instabilities and capillary flows, interfacial oscillators, effects of gravity jitter on typical fluid science experiments and on natural convection in a vertical cylinder. Also discussed are: double-diffusive convection and its effects under reduced gravity, segregation and convection in dendritic alloys, fluid flow and microstructure development, analysis of convective situations with themore » Soret effect, complex natural convection in low Prandtl number metals, separation physics, phase partitioning in reduced gravity, separation of binary alloys with miscibility gap in the melt, Ostwald ripening in liquids, particle cloud combustion in reduced gravity, opposed-flow flame spread with implications for combustion at microgravity.« less

Capillary Thinning and Pinch-off Dynamics and Printability of Polyelectrolyte Solutions

NASA Astrophysics Data System (ADS)

Sharma, Vivek; Jimenez, Leidy N.; Dinic, Jelena; Parsi, Nikila

Biological macromolecules like proteins, DNA and polysaccharides, and many industrial polymers, are classified together as polyelectrolytes for in solution, the repeat units in their backbone are decorated with disassociated, charge-bearing ionic groups, surrounded by counter-ions. In diverse applications like inkjet printing, sprayable cosmetics and insecticides, paints and coatings that involve formation of fluid columns or sheets that undergo progressive thinning and pinch-off into drops, the dominant flow within the necking filament is extensional in nature. The extensional rheology response of the charged macromolecular solutions is not as well understood as that of their uncharged counterparts. Here focus on the characterization of capillary thinning and pinch-off dynamics, extensional rheology and printability of two model systems: sodium (polystyrene sulfonate) and poly(acrylic acid) by using dripping-onto-substrate (DoS) rheometry technique. Both the measured extensional relaxation times and the extensional viscosity values show salt- and polymer concentration-dependent behavior that is not expected or anticipated from the typical shear rheology response.

Computational fluid dynamics assisted characterization of parafoveal hemodynamics in normal and diabetic eyes using adaptive optics scanning laser ophthalmoscopy

PubMed Central

Lu, Yang; Bernabeu, Miguel O.; Lammer, Jan; Cai, Charles C.; Jones, Martin L.; Franco, Claudio A.; Aiello, Lloyd Paul; Sun, Jennifer K.

2016-01-01

Diabetic retinopathy (DR) is the leading cause of visual loss in working-age adults worldwide. Previous studies have found hemodynamic changes in the diabetic eyes, which precede clinically evident pathological alterations of the retinal microvasculature. There is a pressing need for new methods to allow greater understanding of these early hemodynamic changes that occur in DR. In this study, we propose a noninvasive method for the assessment of hemodynamics around the fovea (a region of the eye of paramount importance for vision). The proposed methodology combines adaptive optics scanning laser ophthalmoscopy and computational fluid dynamics modeling. We compare results obtained with this technique with in vivo measurements of blood flow based on blood cell aggregation tracking. Our results suggest that parafoveal hemodynamics, such as capillary velocity, wall shear stress, and capillary perfusion pressure can be noninvasively and reliably characterized with this method in both healthy and diabetic retinopathy patients. PMID:28078170

Observation of Dipolar Spin-Exchange Interactions with Polar Molecules in a Lattice

DTIC Science & Technology

2013-01-01

extend beyond nearest neighbours. This allows coherent spin dynamics to persist even for gases with relatively high entropy and low lattice filling...dynamics to persist even for gases with relatively high entropy and low lat- tice filling. While measured effects of dipolar interactions in ultracold...limits superexchange to nearest-neighbor interactions and requires extremely low temperature and entropy . In contrast, long-range dipolar

Dynamic Analysis of Gene Expression in Rice Superior and Inferior Grains by RNA-Seq

PubMed Central

Sun, Hongzheng; Peng, Ting; Zhao, Yafan; Du, Yanxiu; Zhang, Jing; Li, Junzhou; Xin, Zeyu; Zhao, Quanzhi

2015-01-01

Poor grain filling of inferior grains located on lower secondary panicle branch causes great drop in rice yield and quality. Dynamic gene expression patterns between superior and inferior grains were examined from the view of the whole transcriptome by using RNA-Seq method. In total, 19,442 genes were detected during rice grain development. Genes involved in starch synthesis, grain storage and grain development were interrogated in particular in superior and inferior grains. Of the genes involved in sucrose to starch transformation process, most were expressed at lower level in inferior grains at early filling stage compared to that of superior grains. But at late filling stage, the expression of those genes was higher in inferior grains and lower in superior grains. The same trends were observed in the expression of grain storage protein genes. While, evidence that genes involved in cell cycle showed higher expression in inferior grains during whole period of grain filling indicated that cell proliferation was active till the late filling stage. In conclusion, delayed expression of most starch synthesis genes in inferior grains and low capacity of sink organ might be two important factors causing low filling rate of inferior grain at early filling stage, and shortage of carbohydrate supply was a limiting factor at late filling stage. PMID:26355995

Identification and Characterization of microRNAs during Maize Grain Filling

PubMed Central

Lv, Panqing; Peng, Qian; Ding, Dong; Li, Weihua; Tang, Jihua

2015-01-01

The grain filling rate is closely associated with final grain yield of maize during the period of maize grain filling. To identify the key microRNAs (miRNAs) and miRNA-dependent gene regulation networks of grain filling in maize, a deep-sequencing technique was used to research the dynamic expression patternsof miRNAs at four distinct developmental grain filling stages in Zhengdan 958, which is an elite hybrid and cultivated widely in China. The sequencing result showed that the expression amount of almost all miRNAs was changing with the development of the grain filling and formed in seven groups. After normalization, 77 conserved miRNAs and 74 novel miRNAs were co-detected in these four samples. Eighty-one out of 162 targets of the conserved miRNAs belonged to transcriptional regulation (81, 50%), followed by oxidoreductase activity (18, 11%), signal transduction (16, 10%) and development (15, 9%). The result showed that miRNA 156, 393, 396 and 397, with their respective targets, might play key roles in the grain filling rate by regulating maize growth, development and environment stress response. The result also offered novel insights into the dynamic change of miRNAs during the developing process of maize kernels and assistedin the understanding of how miRNAs are functioning about the grain filling rate. PMID:25951054

Identification and Characterization of microRNAs during Maize Grain Filling.

PubMed

Jin, Xining; Fu, Zhiyuan; Lv, Panqing; Peng, Qian; Ding, Dong; Li, Weihua; Tang, Jihua

2015-01-01

The grain filling rate is closely associated with final grain yield of maize during the period of maize grain filling. To identify the key microRNAs (miRNAs) and miRNA-dependent gene regulation networks of grain filling in maize, a deep-sequencing technique was used to research the dynamic expression patterns of miRNAs at four distinct developmental grain filling stages in Zhengdan 958, which is an elite hybrid and cultivated widely in China. The sequencing result showed that the expression amount of almost all miRNAs was changing with the development of the grain filling and formed in seven groups. After normalization, 77 conserved miRNAs and 74 novel miRNAs were co-detected in these four samples. Eighty-one out of 162 targets of the conserved miRNAs belonged to transcriptional regulation (81, 50%), followed by oxidoreductase activity (18, 11%), signal transduction (16, 10%) and development (15, 9%). The result showed that miRNA 156, 393, 396 and 397, with their respective targets, might play key roles in the grain filling rate by regulating maize growth, development and environment stress response. The result also offered novel insights into the dynamic change of miRNAs during the developing process of maize kernels and assisted in the understanding of how miRNAs are functioning about the grain filling rate.

Modeling and Fault Simulation of Propellant Filling System

NASA Astrophysics Data System (ADS)

Jiang, Yunchun; Liu, Weidong; Hou, Xiaobo

2012-05-01

Propellant filling system is one of the key ground plants in launching site of rocket that use liquid propellant. There is an urgent demand for ensuring and improving its reliability and safety, and there is no doubt that Failure Mode Effect Analysis (FMEA) is a good approach to meet it. Driven by the request to get more fault information for FMEA, and because of the high expense of propellant filling, in this paper, the working process of the propellant filling system in fault condition was studied by simulating based on AMESim. Firstly, based on analyzing its structure and function, the filling system was modular decomposed, and the mathematic models of every module were given, based on which the whole filling system was modeled in AMESim. Secondly, a general method of fault injecting into dynamic system was proposed, and as an example, two typical faults - leakage and blockage - were injected into the model of filling system, based on which one can get two fault models in AMESim. After that, fault simulation was processed and the dynamic characteristics of several key parameters were analyzed under fault conditions. The results show that the model can simulate effectively the two faults, and can be used to provide guidance for the filling system maintain and amelioration.

Characterization and Expression Patterns of microRNAs Involved in Rice Grain Filling

PubMed Central

Du, Yanxiu; Zhang, Jing; Li, Junzhou; Liu, Yanxia; Zhao, Yafan; Zhao, Quanzhi

2013-01-01

MicroRNAs (miRNAs) are upstream gene regulators of plant development and hormone homeostasis through their directed cleavage or translational repression of the target mRNAs, which may play crucial roles in rice grain filling and determining the final grain weight and yield. In this study, high-throughput sequencing was performed to survey the dynamic expressions of miRNAs and their corresponding target genes at five distinct developmental stages of grain filling. In total, 445 known miRNAs and 45 novel miRNAs were detected with most of them expressed in a developmental stage dependent manner, and the majority of known miRNAs, which increased gradually with rice grain filling, showed negatively related to the grain filling rate. Detailed expressional comparisons revealed a clear negative correlation between most miRNAs and their target genes. It was found that specific miRNA cohorts are expressed in a developmental stage dependent manner during grain filling and the known functions of these miRNAs are involved in plant hormone homeostasis and starch accumulation, indicating that the expression dynamics of these miRNAs might play key roles in regulating rice grain filling. PMID:23365650

Dynamic analysis of bulk-fill composites: Effect of food-simulating liquids.

PubMed

Eweis, Ahmed Hesham; Yap, Adrian U-Jin; Yahya, Noor Azlin

2017-10-01

This study investigated the effect of food simulating liquids on visco-elastic properties of bulk-fill restoratives using dynamic mechanical analysis. One conventional composite (Filtek Z350 [FZ]), two bulk-fill composites (Filtek Bulk-fill [FB] and Tetric N Ceram [TN]) and a bulk-fill giomer (Beautifil-Bulk Restorative [BB]) were evaluated. Specimens (12 × 2 × 2mm) were fabricated using customized stainless steel molds. The specimens were light-cured, removed from their molds, finished, measured and randomly divided into six groups. The groups (n = 10) were conditioned in the following mediums for 7 days at 37°C: air (control), artificial saliva (SAGF), distilled water, 0.02N citric acid, heptane, 50% ethanol-water solution. Specimens were assessed using dynamic mechanical testing in flexural three-point bending mode and their respective mediums at 37°C and a frequency range of 0.1-10Hz. The distance between the supports were fixed at 10mm and an axial load of 5N was employed. Data for elastic modulus, viscous modulus and loss tangent were subjected to ANOVA/Tukey's tests at significance level p < 0.05. Significant differences in visco-elastic properties were observed between materials and mediums. Apart from bulk-fill giomer, elastic modulus was the highest after conditioning in heptane. No apparent trends were noted for viscous modulus. Generally, loss tangent was the highest after conditioning in ethanol. The effect of food-simulating liquids on the visco-elastic properties of bulk-fill composites was material and medium dependent. Copyright © 2017 Elsevier Ltd. All rights reserved.

Ionic charge accumulation at microscopic interfaces in filled composites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhu Yutao; Wang Xinheng; Xie Hengkun

1996-12-31

In this paper the charge accumulation process at microscopic interfaces in insulating materials filled with inorganic fillers is analyzed by using a unit model. Dynamic equations of interfacial ionic charge accumulation are proposed by the authors. The charge accumulation and its regulations are proved by TSC test results obtained on silica filled EPDM samples.

Method for making alignment-enhancing feed-through conductors for stackable silicon-on-sapphire

NASA Technical Reports Server (NTRS)

Anthony, Thomas R. (Inventor)

1985-01-01

Alignment-enhancing electrically conductive feed-through paths are provided for the high-speed low-loss transfer of electrical signals between integrated circuits of a plurality of silicon-on-sapphire bodies arrayed in a stack. The alignment-enhancing feed-throughs are made by a process of this invention involving the drilling of holes through the body, double-sided sputtering, electroplating, and the filling of the holes with solder by capillary action. The alignment-enhancing feed-throughs are activated by forming a stack of wafers and remelting the solder whereupon the wafers, and the feed-through paths, are pulled into alignment by surface tension forces.

Capillarity-induced disassembly of virions in carbon nanotubes

NASA Astrophysics Data System (ADS)

Fan, Xiaobin; Barclay, J. Elaine; Peng, Wenchao; Li, Yang; Li, Xianyu; Zhang, Guoliang; Evans, David J.; Zhang, Fengbao

2008-04-01

Studying the transport and fate of viruses through nanochannels is of great importance. By using the nanochannel of a carbon nanotube (CNT) as an ideal model, we evaluated the possibility of capillarity-induced viral transport through a closely fitting nanochannel and explored the mechanisms involved. It is shown both experimentally and theoretically that Cowpea mosaic virus can enter CNTs by capillarity. However, when introduced into a nanotube the protein capsid may disassemble. During the initial capillary filling stage, anomalous needle-shaped high pressure exists in the centre of the nanotube's entrance. This high pressure, combining with the significant negative pressure within the nanotube, may account for the disassembly of the virions.

Experimental characterization of active plasma lensing for electron beams

NASA Astrophysics Data System (ADS)

Pompili, R.; Anania, M. P.; Bellaveglia, M.; Biagioni, A.; Bini, S.; Bisesto, F.; Brentegani, E.; Castorina, G.; Chiadroni, E.; Cianchi, A.; Croia, M.; Di Giovenale, D.; Ferrario, M.; Filippi, F.; Giribono, A.; Lollo, V.; Marocchino, A.; Marongiu, M.; Mostacci, A.; Di Pirro, G.; Romeo, S.; Rossi, A. R.; Scifo, J.; Shpakov, V.; Vaccarezza, C.; Villa, F.; Zigler, A.

2017-03-01

The active plasma lens represents a compact and affordable tool with radially symmetric focusing and field gradients up to several kT/m. In order to be used as a focusing device, its effects on the particle beam distribution must be well characterized. Here, we present the experimental results obtained by focusing an high-brightness electron beam by means of a 3 cm-long discharge-capillary pre-filled with Hydrogen gas. We achieved minimum spot sizes of 24 μ m (rms) showing that, during plasma lensing, the beam emittance increases due to nonlinearities in the focusing field. The results have been cross-checked with numerical simulations, showing an excellent agreement.

Fast-acting self-healing metallic fuse.

NASA Technical Reports Server (NTRS)

Schwartz, F. C.; Renton, C. A.; Rabinovici, B.

1971-01-01

Description of a fast-acting nonmechanical self-healing mercury fuse capable of protecting a high current circuit or device from overcurrent fault damages. Basically the self-healing fuse consists of two enclosed mercury reservoirs connected by a fine capillary tube filled with mercury that serves as the fusing element. It is pointed out that a better understanding of the energy conversion process involved in the operation of the device could help explore other device configurations (such as a tapering geometry and use of magnetic field to drive the arc into the fuse wall on inductive loads, etc.) and thus extend the range of capabilities for this type of protective device.

Nearly 1000 Protein Identifications from 50 ng of Xenopus laevis Zygote Homogenate Using Online Sample Preparation on a Strong Cation Exchange Monolith Based Microreactor Coupled with Capillary Zone Electrophoresis.

PubMed

Zhang, Zhenbin; Sun, Liangliang; Zhu, Guijie; Cox, Olivia F; Huber, Paul W; Dovichi, Norman J

2016-01-05

A sulfonate-silica hybrid strong cation exchange monolith microreactor was synthesized and coupled to a linear polyacrylamide coated capillary for online sample preparation and capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) bottom-up proteomic analysis. The protein sample was loaded onto the microreactor in an acidic buffer. After online reduction, alkylation, and digestion with trypsin, the digests were eluted with 200 mM ammonium bicarbonate at pH 8.2 for CZE-MS/MS analysis using 1 M acetic acid as the background electrolyte. This combination of basic elution and acidic background electrolytes results in both sample stacking and formation of a dynamic pH junction. 369 protein groups and 1274 peptides were identified from 50 ng of Xenopus laevis zygote homogenate, which is comparable with an offline sample preparation method, but the time required for sample preparation was decreased from over 24 h to less than 40 min. Dramatically improved performance was produced by coupling the reactor to a longer separation capillary (∼100 cm) and a Q Exactive HF mass spectrometer. 975 protein groups and 3749 peptides were identified from 50 ng of Xenopus protein using the online sample preparation method.

Sensitive detection of malachite green and crystal violet by nonlinear laser wave mixing and capillary electrophoresis.

PubMed

Maxwell, Eric J; Tong, William G

2016-05-01

An ultrasensitive label-free antibody-free detection method for malachite green and crystal violet is presented using nonlinear laser wave-mixing spectroscopy and capillary zone electrophoresis. Wave-mixing spectroscopy provides a sensitive absorption-based detection method for trace analytes. This is accomplished by forming dynamic gratings within a sample cell, which diffracts light to create a coherent laser-like signal beam with high optical efficiency and high signal-to-noise ratio. A cubic dependence on laser power and square dependence on analyte concentration make wave mixing sensitive enough to detect molecules in their native form without the use of fluorescent labels for signal enhancement. A 532 nm laser and a 635 nm laser were used for malachite green and crystal violet sample excitation. The use of two lasers of different wavelengths allows the method to simultaneously detect both analytes. Selectivity is obtained through the capillary zone electrophoresis separation, which results in characteristic migration times. Measurement in capillary zone electrophoresis resulted in a limit of detection of 6.9 × 10(-10)M (2.5 × 10(-19) mol) for crystal violet and 8.3 × 10(-11)M (3.0 × 10(-20) mol) for malachite green at S/N of 2. Copyright © 2016. Published by Elsevier B.V.

The fabrication of highly ordered block copolymer micellar arrays: control of the separation distances of silicon oxide dots

NASA Astrophysics Data System (ADS)

Yoo, Hana; Park, Soojin

2010-06-01

We demonstrate the fabrication of highly ordered silicon oxide dotted arrays prepared from polydimethylsiloxane (PDMS) filled nanoporous block copolymer (BCP) films and the preparation of nanoporous, flexible Teflon or polyimide films. Polystyrene-block-poly(2-vinylpyridine) (PS-b-P2VP) films were annealed in toluene vapor to enhance the lateral order of micellar arrays and were subsequently immersed in alcohol to produce nano-sized pores, which can be used as templates for filling a thin layer of PDMS. When a thin layer of PDMS was spin-coated onto nanoporous BCP films and thermally annealed at a certain temperature, the PDMS was drawn into the pores by capillary action. PDMS filled BCP templates were exposed to oxygen plasma environments in order to fabricate silicon oxide dotted arrays. By addition of PS homopolymer to PS-b-P2VP copolymer, the separation distances of micellar arrays were tuned. As-prepared silicon oxide dotted arrays were used as a hard master for fabricating nanoporous Teflon or polyimide films by spin-coating polymer precursor solutions onto silicon patterns and peeling off. This simple process enables us to fabricate highly ordered nanoporous BCP templates, silicon oxide dots, and flexible nanoporous polymer patterns with feature size of sub-20 nm over 5 cm × 5 cm.

The fabrication of highly ordered block copolymer micellar arrays: control of the separation distances of silicon oxide dots.

PubMed

Yoo, Hana; Park, Soojin

2010-06-18

We demonstrate the fabrication of highly ordered silicon oxide dotted arrays prepared from polydimethylsiloxane (PDMS) filled nanoporous block copolymer (BCP) films and the preparation of nanoporous, flexible Teflon or polyimide films. Polystyrene-block-poly(2-vinylpyridine) (PS-b-P2VP) films were annealed in toluene vapor to enhance the lateral order of micellar arrays and were subsequently immersed in alcohol to produce nano-sized pores, which can be used as templates for filling a thin layer of PDMS. When a thin layer of PDMS was spin-coated onto nanoporous BCP films and thermally annealed at a certain temperature, the PDMS was drawn into the pores by capillary action. PDMS filled BCP templates were exposed to oxygen plasma environments in order to fabricate silicon oxide dotted arrays. By addition of PS homopolymer to PS-b-P2VP copolymer, the separation distances of micellar arrays were tuned. As-prepared silicon oxide dotted arrays were used as a hard master for fabricating nanoporous Teflon or polyimide films by spin-coating polymer precursor solutions onto silicon patterns and peeling off. This simple process enables us to fabricate highly ordered nanoporous BCP templates, silicon oxide dots, and flexible nanoporous polymer patterns with feature size of sub-20 nm over 5 cm x 5 cm.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dewers, Thomas; Heath, Jason E.; Leigh, Christi D.

The nature of geologic disposal of nuclear waste in salt formations requires validated and verified two - phase flow models of transport of brine and gas through intact, damaged, and consolidating crushed salt. Such models exist in oth er realms of subsurface engineering for other lithologic classes (oil and gas, carbon sequestration etc. for clastics and carbonates) but have never been experimentally validated and parameterized for salt repository scenarios or performance assessment. Mo dels for waste release scenarios in salt back - fill require phenomenological expressions for capillary pressure and relative permeability that are expected to change with degree ofmore » consolidation, and require experimental measurement to parameterize and vali date. This report describes a preliminary assessment of the influence of consolidation (i.e. volume strain or porosity) on capillary entry pressure in two phase systems using mercury injection capillary pressure (MICP). This is to both determine the potent ial usefulness of the mercury intrusion porosimetry method, but also to enable a better experimental design for these tests. Salt consolidation experiments are performed using novel titanium oedometers, or uniaxial compression cells often used in soil mech anics, using sieved run - of - mine salt from the Waste Isolation Pilot Plant (WIPP) as starting material. Twelve tests are performed with various starting amounts of brine pore saturation, with axial stresses up to 6.2 MPa (%7E900 psi) and temperatures to 90 o C. This corresponds to UFD Work Package 15SN08180211 milestone "FY:15 Transport Properties of Run - of - Mine Salt Backfill - Unconsolidated to Consolidated". Samples exposed to uniaxial compression undergo time - dependent consolidation, or creep, to various deg rees. Creep volume strain - time relations obey simple log - time behavior through the range of porosities (%7E50 to 2% as measured); creep strain rate increases with temperature and applied stress as expected. Mercury porosimetry is used to determine characteri stic capillary pressure curves from a series of consolidation tests and show characteristic saturation - capillary pressure curves that follow the common van Genuchten (1978, 1980) formulation at low stresses. Higher capillary pressure data are suspect due t o the large potential for sample damage, including fluid inclusion decrepitation and pore collapse. Data are supportive of use of the Leverett "J" function (Leverett, 1941) to use for scaling characteristic curves at different degrees of consolidation, but better permeability determinations are needed to support this hypothesis. Recommendations for further and refined testing are made with the goal of developing a self - consistent set of constitutive laws for granular salt consolidation and multiphase (brin e - air) flow.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dewers, Thomas; Heath, Jason E.; Leigh, Christi D.

The nature of geologic disposal of nuclear waste in salt formations requires validated and verified two-phase flow models of transport of brine and gas through intact, damaged, and consolidating crushed salt. Such models exist in other realms of subsurface engineering for other lithologic classes (oil and gas, carbon sequestration etc. for clastics and carbonates) but have never been experimentally validated and parameterized for salt repository scenarios or performance assessment. Models for waste release scenarios in salt back-fill require phenomenological expressions for capillary pressure and relative permeability that are expected to change with degree of consolidation, and require experimental measurement tomore » parameterize and validate. This report describes a preliminary assessment of the influence of consolidation (i.e. volume strain or porosity) on capillary entry pressure in two phase systems using mercury injection capillary pressure (MICP). This is to both determine the potential usefulness of the mercury intrusion porosimetry method, but also to enable a better experimental design for these tests. Salt consolidation experiments are performed using novel titanium oedometers, or uniaxial compression cells often used in soil mechanics, using sieved run-of-mine salt from the Waste Isolation Pilot Plant (WIPP) as starting material. Twelve tests are performed with various starting amounts of brine pore saturation, with axial stresses up to 6.2 MPa (~900 psi) and temperatures to 90°C. This corresponds to UFD Work Package 15SN08180211 milestone “FY:15 Transport Properties of Run-of-Mine Salt Backfill – Unconsolidated to Consolidated”. Samples exposed to uniaxial compression undergo time-dependent consolidation, or creep, to various degrees. Creep volume strain-time relations obey simple log-time behavior through the range of porosities (~50 to 2% as measured); creep strain rate increases with temperature and applied stress as expected. Mercury porosimetry is used to determine characteristic capillary pressure curves from a series of consolidation tests and show characteristic saturation-capillary pressure curves that follow the common van Genuchten (1978, 1980) formulation at low stresses. Higher capillary pressure data are suspect due to the large potential for sample damage, including fluid inclusion decrepitation and pore collapse. Data are supportive of use of the Leverett “J” function (Leverett, 1941) to use for scaling characteristic curves at different degrees of consolidation, but better permeability determinations are needed to support this hypothesis. Recommendations for further and refined testing are made with the goal of developing a self- consistent set of constitutive laws for granular salt consolidation and multiphase (brine-air) flow.« less

Surface structure of imidazolium-based ionic liquids: Quantitative comparison between simulations and high-resolution RBS measurements.

PubMed

Nakajima, Kaoru; Nakanishi, Shunto; Lísal, Martin; Kimura, Kenji

2016-03-21

Elemental depth profiles of 1-alkyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([CnMIM][TFSI], n = 4, 6, 8) are measured using high-resolution Rutherford backscattering spectroscopy (HRBS). The profiles are compared with the results of molecular dynamics (MD) simulations. Both MD simulations and HRBS measurements show that the depth profiles deviate from the uniform stoichiometric composition in the surface region, showing preferential orientations of ions at the surface. The MD simulations qualitatively reproduce the observed HRBS profiles but the agreement is not satisfactory. The observed discrepancy is ascribed to the capillary waves. By taking account of the surface roughness induced by the capillary waves, the agreement becomes almost perfect.

Surface capillary currents: Rediscovery of fluid-structure interaction by forced evolving boundary theory

NASA Astrophysics Data System (ADS)

Wang, Chunbai; Mitra, Ambar K.

2016-01-01

Any boundary surface evolving in viscous fluid is driven with surface capillary currents. By step function defined for the fluid-structure interface, surface currents are found near a flat wall in a logarithmic form. The general flat-plate boundary layer is demonstrated through the interface kinematics. The dynamics analysis elucidates the relationship of the surface currents with the adhering region as well as the no-slip boundary condition. The wall skin friction coefficient, displacement thickness, and the logarithmic velocity-defect law of the smooth flat-plate boundary-layer flow are derived with the advent of the forced evolving boundary method. This fundamental theory has wide applications in applied science and engineering.

Surface structure of imidazolium-based ionic liquids: Quantitative comparison between simulations and high-resolution RBS measurements

NASA Astrophysics Data System (ADS)

Nakajima, Kaoru; Nakanishi, Shunto; Lísal, Martin; Kimura, Kenji

2016-03-01

Elemental depth profiles of 1-alkyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([CnMIM][TFSI], n = 4, 6, 8) are measured using high-resolution Rutherford backscattering spectroscopy (HRBS). The profiles are compared with the results of molecular dynamics (MD) simulations. Both MD simulations and HRBS measurements show that the depth profiles deviate from the uniform stoichiometric composition in the surface region, showing preferential orientations of ions at the surface. The MD simulations qualitatively reproduce the observed HRBS profiles but the agreement is not satisfactory. The observed discrepancy is ascribed to the capillary waves. By taking account of the surface roughness induced by the capillary waves, the agreement becomes almost perfect.

Using Molecular Dynamics Simulation to Reinforce Student Understanding of Intermolecular Forces

ERIC Educational Resources Information Center

Burkholder, Phillip R.; Purser, Gordon H.; Cole, Renee S.

2008-01-01

Intermolecular forces play an important role in many aspects of chemistry ranging from inorganic to biological chemistry. These forces dictate molecular conformation, species aggregation (including self-assembly), trends in solubility and boiling points, adsorption characteristics, viscosity, phase changes, surface tension, capillary action, vapor…

NASA LeRC's Acoustic Fill Effect Test Program and Results

NASA Technical Reports Server (NTRS)

Hughes, William O.; Mcnelis, Mark E.; Manning, Jerome E.

1994-01-01

NASA Lewis Research Center, in conjunction with General Dynamics Space Systems Division, has performed a test program to investigate the acoustic fill effect for an unblanketed payload fairing for a variety of payload simulators. This paper will discuss this test program and fill factor test data, and make comparisons with theoretical predictions. This paper will also address the NASA acoustic fill effect standard which was verified from the test data analysis.

Drop Weight Impact Behavior of Al-Si-Cu Alloy Foam-Filled Thin-Walled Steel Pipe Fabricated by Friction Stir Back Extrusion

NASA Astrophysics Data System (ADS)

Hangai, Yoshihiko; Nakano, Yukiko; Utsunomiya, Takao; Kuwazuru, Osamu; Yoshikawa, Nobuhiro

2017-02-01

In this study, Al-Si-Cu alloy ADC12 foam-filled thin-walled stainless steel pipes, which exhibit metal bonding between the ADC12 foam and steel pipe, were fabricated by friction stir back extrusion. Drop weight impact tests were conducted to investigate the deformation behavior and mechanical properties of the foam-filled pipes during dynamic compression tests, which were compared with the results of static compression tests. From x-ray computed tomography observation, it was confirmed that the fabricated foam-filled pipes had almost uniform porosity and pore size distributions. It was found that no scattering of the fragments of collapsed ADC12 foam occurred for the foam-filled pipes owing to the existence of the pipe surrounding the ADC12 foam. Preventing the scattering of the ADC12 foam decreases the drop in stress during dynamic compression tests and therefore improves the energy absorption properties of the foam.

Chromatography, solid-phase extraction, and capillary electrochromatography with MIPs.

PubMed

Tóth, Blanka; Horvai, George

2012-01-01

Most analytical applications of molecularly imprinted polymers are based on their selective adsorption properties towards the template or its analogs. In chromatography, solid phase extraction and electrochromatography this adsorption is a dynamic process. The dynamic process combined with the nonlinear adsorption isotherm of the polymers and other factors results in complications which have limited the success of imprinted polymers. This chapter explains these problems and shows many examples of successful applications overcoming or avoiding the problems.

Combination of ICP-MS, capillary electrophoresis, and their hyphenation for probing Ru(III) metallodrug-DNA interactions.

PubMed

Foteeva, Lidia S; Matczuk, Magdalena; Pawlak, Katarzyna; Aleksenko, Svetlana S; Nosenko, Sergey V; Karandashev, Vasily K; Jarosz, Maciej; Timerbaev, Andrei R

2017-03-01

Determination of the DNA-binding reactivity and affinity is an important part of a successful program for the selection of metallodrug candidates. For such assaying, a range of complementary analytical techniques was proposed and tested here using one of few anticancer metal-based drugs that are currently in clinical trials, indazolium trans-[tetrachloridobis(1H-indazole)ruthenate(III), and a DNA oligonucleotide. A high reactivity of the Ru drug was confirmed in affinity capillary electrophoresis (CE) mode, where adduct formation takes place in situ (i.e., in the capillary filled with an oligonucleotide-containing electrolyte). To further characterize the binding kinetics, a drug-oligonucleotide mixture was incubated for a different period of time, followed by ultrafiltration separation into two different in molecular weight fractions (>3 and <3 kDa). The time-dependent distribution profiles of the Ru drug were then assessed by CE-inductively coupled plasma mass spectrometry (ICP-MS), revealing that at least two DNA adducts exist at equilibrium conditions. Using standalone ICP-MS, dominant equilibrium amount of the bound ruthenium was found to occur in a fraction of 5-10 kDa, which includes the oligonucleotide (ca. 6 kDa). Importantly, in all three assays, the drug was used for the first time in in-vitro studies, not in the intact form but as its active species released from the transferrin adduct at simulated cancer cytosolic conditions. This circumstance makes the established analytical platform promising to provide a detailed view on metallodrug targeting, including other possible biomolecules and ex vivo samples.

A new model for studying the revascularization of skin grafts in vivo: the role of angiogenesis.

PubMed

Lindenblatt, Nicole; Calcagni, Maurizio; Contaldo, Claudio; Menger, Michael D; Giovanoli, Pietro; Vollmar, Brigitte

2008-12-01

Models of skin graft revascularization are based mostly on histologic evaluations but lack the possibility of analyzing the vascular biology in vivo. The aim of the present study was therefore to develop an animal model that allows continuous monitoring of the microcirculation during skin graft healing. Skin and subcutaneous tissue were removed from the back of dorsal skinfold chamber preparations in mice, leaving one layer of striated muscle and subcutaneous tissue as a wound bed (n = 5). A corresponding full-thickness skin graft was harvested from the groin and sutured into the defect in the back of the chamber. To study graft healing, repetitive intravital microscopy was performed during the first 10 days after engraftment. Capillary widening in the wound bed appeared at day 1 after grafting and increased until day 4. Capillary buds and sprouts first appeared at day 2. Blood filling of autochthonous graft capillaries occurred at day 3, resulting in almost complete restoration of the original skin microcirculation on day 5. This was achieved by interconnections between the microvasculature of the wound bed and the skin graft through a temporary angiogenic response. In principle, angiogenic blood vessel growth originated in the wound bed and was directed toward the graft. This new model allows for repetitive analysis of the microcirculation during skin graft healing. It provides ideal in vivo conditions to further delineate the exact mechanisms of blood vessel interconnection during the complex process of angiogenesis, and may also allow study of the vascularization of tissue-engineered skin substitutes.

DNA migration mechanism analyses for applications in capillary and microchip electrophoresis

PubMed Central

Forster, Ryan E.; Hert, Daniel G.; Chiesl, Thomas N.; Fredlake, Christopher P.; Barron, Annelise E.

2009-01-01

In 2009, electrophoretically driven DNA separations in slab gels and capillaries have the sepia tones of an old-fashioned technology in the eyes of many, even while they remain ubiquitously used, fill a unique niche, and arguably have yet to reach their full potential. For comic relief, what is old becomes new again: agarose slab gel separations are used to prepare DNA samples for “next-gen” sequencing platforms (e.g., the Illumina and 454 machines)—dsDNA molecules within a certain size range are “cut out” of a gel and recovered for subsequent “massively parallel” pyrosequencing. In this review, we give a Barron lab perspective on how our comprehension of DNA migration mechanisms in electrophoresis has evolved, since the first reports of DNA separations by CE (∼1989) until now, 20 years later. Fused silica capillaries, and borosilicate glass and plastic microchips, quietly offer increasing capacities for fast (and even “ultra-fast”), efficient DNA separations. While the channel-by-channel scaling of both old and new electrophoresis platforms provides key flexibility, it requires each unique DNA sample to be prepared in its own micro- or nanovolume. This Achille's heel of electrophoresis technologies left an opening through which pooled-sample, next-gen DNA sequencing technologies rushed. We shall see, over time, whether sharpening understanding of transitions in DNA migration modes in crosslinked gels, nanogel solutions, and uncrosslinked polymer solutions will allow electrophoretic DNA analysis technologies to flower again. Microchannel electrophoresis, after a quiet period of metamorphosis, may emerge sleeker and more powerful, to claim its own important niche applications. PMID:19582705

Contact Line Instability Caused by Air Rim Formation under Nonsplashing Droplets.

PubMed

Pack, Min; Kaneelil, Paul; Kim, Hyoungsoo; Sun, Ying

2018-05-01

Drop impact is fundamental to various natural and industrial processes such as rain-induced soil erosion and spray-coating technologies. The recent discovery of the role of air entrainment between the droplet and the impacting surface has produced numerous works, uncovering the unique physics that correlates the air film dynamics with the drop impact outcomes. In this study, we focus on the post-failure air entrainment dynamics for We numbers well below the splash threshold under different ambient pressures and elucidate the interfacial instabilities formed by air entrainment at the wetting front of impacting droplets on perfectly smooth, viscous films of constant thickness. A high-speed total internal reflection microscopy technique accounting for the Fresnel reflection at the drop-air interface allows for in situ measurements of an entrained air rim at the wetting front. The presence of an air rim is found to be a prerequisite to the interfacial instability which is formed when the capillary pressure in the vicinity of the contact line can no longer balance the increasing gas pressure near the wetting front. A critical capillary number for the air rim formation is experimentally identified above which the wetting front becomes unstable where this critical capillary number inversely scales with the ambient pressure. The contact line instabilities at relatively low We numbers ( We ∼ O(10)) observed in this study provide insight into the conventional understanding of hydrodynamic instabilities under drop impact which usually require We ≫ 10.

Surface roughness effects on contact line motion with small capillary number

NASA Astrophysics Data System (ADS)

Yang, Feng-Chao; Chen, Xiao-Peng; Yue, Pengtao

2018-01-01

In this work, we investigate how surface roughness influences contact line dynamics by simulating forced wetting in a capillary tube. The tube wall is decorated with microgrooves and is intrinsically hydrophilic. A phase-field method is used to capture the fluid interface and the moving contact line. According to the numerical results, a criterion is proposed to judge whether the grooves are entirely wetted or not at vanishing capillary numbers. When the contact line moves over a train of grooves, the apparent contact angle exhibits a periodic nature, no matter whether the Cassie-Baxter or the Wenzel state is achieved. The oscillation amplitude of apparent contact angle is analyzed and found to be inversely proportional to the interface area. The contact line motion can be characterized as stick-jump-slip in the Cassie-Baxter state and stick-slip in the Wenzel state. By comparing to the contact line dynamics on smooth surfaces, equivalent microscopic contact angles and slip lengths are obtained. The equivalent slip length in the Cassie-Baxter state agrees well with the theoretical model in the literature. The equivalent contact angles are, however, much greater than the predictions of the Cassie-Baxter model and the Wenzel model for equilibrium stable states. Our results reveal that the pinning of the contact line at surface defects effectively enhances the hydrophobicity of rough surfaces, even when the surface material is intrinsically hydrophilic and the flow is under the Wenzel state.

Comparative study on occurrence characteristics of matrix water in static and gas double-dynamic solid-state fermentations using low-field NMR and MRI.

PubMed

He, Qin; Chen, Hong-zhang

2015-12-01

The water in a solid substrate is generally divided into three forms: hygroscopic, capillary, and free. However, there are few methods available for detecting the contents of different states of water in substrates. In this paper, low-field NMR and MRI were used to analyze the water occurrence characteristics of steam-exploded corn straw in solid-state fermentation (SSF). A significant linear relationship was found between the total NMR peak areas and the total water contents with a correlation coefficient of 0.993. It was further proved to be successful in comparing the contents and distributions of different states of water in static SSF and gas double-dynamic SSF (GDD-SSF). The results showed that among the three states of water, capillary water was the main form of water present and lost in substrates during fermentation. Total water and capillary water contents did not significantly differ as a result of different sample treatments, but hygroscopic water and free water contents in static SSF were respectively 0.38 and 2.98 times that in GDD-SSF with a packing height of 3 cm after fermentation. A relatively uniform water distribution and deep-depth region for microbial growth were found in GDD-SSF, suggesting that GDD-SSF was more suitable for industrialization. This technology has great potential for achieving efficient on-line water supply through water loss detection in SSF.

Composition of the C6+ Fraction of Natural Gas by Multiple Porous Layer Open Tubular Capillaries Maintained at Low Temperatures.

PubMed

Burger, Jessica L; Lovestead, Tara M; Bruno, Thomas J

2016-03-17

As the sources of natural gas become more diverse, the trace constituents of the C 6 + fraction are of increasing interest. Analysis of fuel gas (including natural gas) for compounds with more than 6 carbon atoms (the C 6 + fraction) has historically been complex and expensive. Hence, this is a procedure that is used most often in troubleshooting rather than for day-to-day operations. The C 6 + fraction affects gas quality issues and safety considerations such as anomalies associated with odorization. Recent advances in dynamic headspace vapor collection can be applied to this analysis and provide a faster, less complex alternative for compositional determination of the C 6 + fraction of natural gas. Porous layer open tubular capillaries maintained at low temperatures (PLOT-cryo) form the basis of a dynamic headspace sampling method that was developed at NIST initially for explosives in 2009. This method has been recently advanced by the combining of multiple PLOT capillary traps into one "bundle," or wafer, resulting in a device that allows the rapid trapping of relatively large amounts of analyte. In this study, natural gas analytes were collected by flowing natural gas from the laboratory (gas out of the wall) or a prepared surrogate gas flowing through a chilled wafer. The analytes were then removed from the PLOT-cryo wafer by thermal desorption and subsequent flushing of the wafer with helium. Gas chromatography (GC) with mass spectrometry (MS) was then used to identify the analytes.

Dynamic cerebral autoregulation measured with coherent hemodynamics spectroscopy (CHS)

NASA Astrophysics Data System (ADS)

Kainerstorfer, Jana M.; Sassaroli, Angelo; Tgavalekos, Kristen T.; Fantini, Sergio

2015-03-01

Coherent Hemodynamics Spectroscopy (CHS) is a novel technique for non-invasive measurements of local microcirculation quantities such as the capillary blood transit times and dynamic autoregulation. The basis of CHS is to measure, for instance with near-infrared spectroscopy (NIRS), peripheral coherent hemodynamic changes that are induced by controlled perturbations in the systemic mean arterial pressure (MAP). In this study, the MAP perturbation was induced by the fast release of two pneumatic cuffs placed around the subject's thighs after they were kept inflated (at 200 mmHg) for two minutes. The resulting transient changes in cerebral oxy- (O) and deoxy- (D) hemoglobin concentrations measured with NIRS on the prefrontal cortex are then described by a novel hemodynamic model, from which quantifiable parameters such as the capillary blood transit time and a cutoff frequency for cerebral autoregulation are obtained. We present results on eleven healthy volunteers in a protocol involving measurements during normal breathing and during hyperventilation, which is known to cause a hypocapnia-induced increase in cerebral autoregulation. The measured capillary transit time was unaffected by hyperventilation (normal breathing: 1.1±0.1 s; hyperventilation: 1.1±0.1 s), whereas the cutoff frequency of autoregulation, which increases for higher autoregulation efficiency, was indeed found to be significantly greater during hyperventilation (normal breathing: 0.017±0.002 Hz; hyperventilation: 0.034±0.005 Hz). These results provide a validation of local cerebral autoregulation measurements with the new technique of CHS.

Optically Reconfigurable Chiral Microspheres of Self-Organized Helical Superstructures with Handedness Inversion.

PubMed

Wang, Ling; Chen, Dong; Gutierrez-Cuevas, Karla G; Bisoyi, Hari Krishna; Fan, Jing; Zola, Rafael S; Li, Guoqiang; Urbas, Augustine M; Bunning, Timothy J; Weitz, David A; Li, Quan

2017-01-01

Optically reconfigurable monodisperse chiral microspheres of self-organized helical superstructures with dynamic chirality were fabricated via a capillary-based microfluidic technique. Light-driven handedness-invertible transformations between different configurations of microspheres were vividly observed and optically tunable RGB photonic cross-communications among the microspheres were demonstrated.

Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection.

PubMed

Duffy, Ciarán F; MacCraith, Brian; Diamond, Dermot; O'Kennedy, Richard; Arriaga, Edgar A

2006-08-01

The analysis of mitochondria by capillary electrophoresis usually takes longer than 20 min per replicate which may compromise the quality of the mitochondria due to degradation. In addition, low sample consumption may be beneficial in the analysis of rare or difficult samples. In this report, we demonstrate the ability to analyze individual mitochondrial events in picoliter-volume samples (approximately 80 pL) taken from a bovine liver preparation using microchip capillary electrophoresis with laser-induced fluorescence detection (micro-chip CE-LIF). Using a commercial "double-T" glass microchip, the sample was electrokinetically loaded in the "double-T" intersection and then subjected to electrophoretic separation along the main separation channel. In order to decrease interactions of mitochondria with channel walls during the analysis, poly(vinyl alcohol) was used as a dynamic coating. This procedure eliminates the need for complicated covalent surface modifications within the channels that were previously used in capillary electrophoresis methods. For analysis, mitochondria, isolated from bovine liver tissue, were selectively labelled using 10-nonyl acridine orange (NAO). The results consist of electropherograms where each mitochondrial event is a narrow spike (240 +/- 44 ms). While the spike intensity is representative of its NAO content, its migration time is used to calculate and describe its electrophoretic mobility, which is a property still largely unexplored for intracellular organelles. The five-fold decrease in separation time (4 min for microchip versus 20 min for capillary electrophoresis) makes microchip electrophoretic separations of organelles a faster, sensitive, low-sample volume alternative for the characterization of individual organelle properties and for investigations of subcellular heterogeneity.

Effect of Marangoni Convection on Surfactant Transfer Between the Drop Connected to the Reservoir and Surrounding Liquid

NASA Astrophysics Data System (ADS)

Kostarev, K.; Denisova, M.; Shmyrov, A.

2018-03-01

The paper presents the results of comparative investigation of the interaction between the capillary and buoyant mechanisms of motion in a problem of surfactant mass transfer between an insoluble drop and surrounding fluid under different gravity conditions. The research was performed for the drop that is coupled with the reservoir filled with a source mixture through a long thin tube (needle). Visualization of the flow patterns and concentration fields has shown that surfactant diffusion from the needle at normal gravity leads to the onset of the oscillatory mode of the capillary convection in the drop. It has been found that the frequency of the Marangoni convection outbursts, the lifetime of the oscillatory flow modes and the amount of the source mixture involved in the process of mass transfer depend on the drop size and initial concentration of the surfactant. The obtained results are compared with the cases of surfactant diffusion from the isolated drop under terrestrial conditions and from the drop coupled with reservoir in microgravity. Additionally, a series of experiments were performed to investigate diffusion of a surfactant from the surrounding solution into a drop.

Sorptivity of rocks and soils of the van Genuchten-Mualem type

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zimmerman, R.W.; Bodvarsson, G.S.

1991-06-01

One hydrological process that will have great relevance to the performance of the proposed underground radioactive waste repository at Yucca Mountain, Nevada, is that of the absorption of water from a water-filled fracture into the adjacent unsaturated rock formation. The rate at which water is imbibed by a rock depends on the hydrological properties of the rock and on the initial saturation (or initial capillary suction) of the formation. The hydrological properties that affect imbibition are the relative permeability function and the capillary pressure function. These functions are often collectively referred to as the `characteristic functions` of the porous medium.more » For one-dimensional absorption, it can be shown that, regardless of the details of the characteristic functions, the total amount of water imbibed by the formation, per unit surface area, will be proportional to the square root of the elapsed time. Hence the ability of a rock or soil to imbibe water can be quantified by a parameter known as the sorptivity S, which is defined such that the cumulative volumetric liquid influx per unit area is given by Q = S{radical}t. The paper discusses the simplification of these characteristic functions of porous medium.« less

Surfactants non-monotonically modify the onset of Faraday waves

NASA Astrophysics Data System (ADS)

Strickland, Stephen; Shearer, Michael; Daniels, Karen

2017-11-01

When a water-filled container is vertically vibrated, subharmonic Faraday waves emerge once the driving from the vibrations exceeds viscous dissipation. In the presence of an insoluble surfactant, a viscous boundary layer forms at the contaminated surface to balance the Marangoni and Boussinesq stresses. For linear gravity-capillary waves in an undriven fluid, the surfactant-induced boundary layer increases the amount of viscous dissipation. In our analysis and experiments, we consider whether similar effects occur for nonlinear Faraday (gravity-capillary) waves. Assuming a finite-depth, infinite-breadth, low-viscosity fluid, we derive an analytic expression for the onset acceleration up to second order in ɛ =√{ 1 / Re } . This expression allows us to include fluid depth and driving frequency as parameters, in addition to the Marangoni and Boussinesq numbers. For millimetric fluid depths and driving frequencies of 30 to 120 Hz, our analysis recovers prior numerical results and agrees with our measurements of NBD-PC surfactant on DI water. In both case, the onset acceleration increases non-monotonically as a function of Marangoni and Boussinesq numbers. For shallower systems, our model predicts that surfactants could decrease the onset acceleration. DMS-0968258.

Stabilization of a capillary bridge far beyond the Rayleigh--Plateau limit using active feedback and acoustic radiation pressure.

NASA Astrophysics Data System (ADS)

Marr-Lyon, Mark J.; Thiessen, David B.; Marston, Philip L.

1997-11-01

A liquid bridge between two solid surfaces is known as a capillary bridge. For a cylindrical bridge in low gravity of radius R and length L, the slenderness S=L/2R has a natural (Rayleigh--Plateau) limit of π beyond which the bridge breaks. Using the radiation pressure of an ultrasonic standing wave to control the shape of the bridge and an optical sensor to detect the shape of the bridge, an active feedback system was constructed that stabilized bridges significantly beyond the Rayleigh limit in simulated low gravity(Marr--Lyon, M. J., phet al., J. Fluid Mech.), accepted for publication.. The Plateau tank which contained the bridge was a dual frequency ultrasonic resonator and the spatial distribution of the radiation pressure was controlled by adjusting the ultrasonic frequency. Bridges have been extended with S as large as 4.3. To be useful in low gravity, modifications for liquid bridges in air are needed. Acoustic resonators in air having the required property that the sound amplitude can be spatially redistributed rapidly are being investigated using gas-filled soap-film bridges. Work supported by NASA.

Functional integration of PCR amplification and capillary eletrophoresis in a microfabricated DNA analysis device

DOE Office of Scientific and Technical Information (OSTI.GOV)

Woolley, A.T.; deMello, A.J.; Mathies, R.A.

Microfabricated silicon PCR reactors and glass capillary electrophoresis (CE) chips have been successfully coupled to form an integrated DNA analysis system. This construct combines the rapid thermal cycling capabilities of microfabricated PCR devices (10{degree}C/s heating, 2.5{degree}C/s cooling) with the high-speed (<120 s) DNA separations provided by microfabricated CE chips. The PCR chamber and the CE chip were directly linked through a photolithographically fabricated channel filled with hydroxyethylcellulose sieving matrix. Electrophoretic injection directly from the PCR chamber through the cross injection channel was used as an `electrophoretic valve` to couple the PCR and CE devices on-chip. To demonstrate the functionality ofmore » this system, a 15 min PCR amplification of a {Beta}-globin target cloned in m13 was immediately followed by high-speed CE chip separation in under 120 s, providing a rapid PCR-CE analysis in under 20 min. A rapid assay for genomic Salmonella DNA was performed in under 45 min, demonstrating that challenging amplifications of diagnostically interesting targets can also be performed. Real-time monitoring of PCR target amplification in these integrated PCR-CE devices is also feasible. 33 refs., 6 figs.« less

A Study of Crystalline Mechanism of Penetration Sealer Materials.

PubMed

Teng, Li-Wei; Huang, Ran; Chen, Jie; Cheng, An; Hsu, Hui-Mi

2014-01-14

It is quite common to dispense a topping material like crystalline penetration sealer materials (CPSM) onto the surface of a plastic substance such as concrete to extend its service life span by surface protections from outside breakthrough. The CPSM can penetrate into the existing pores or possible cracks in such a way that it may form crystals to block the potential paths which provide breakthrough for any unknown materials. This study investigated the crystalline mechanism formed in the part of concrete penetrated by the CPSM. We analyzed the chemical composites, in order to identify the mechanism of CPSM and to evaluate the penetrated depth. As shown in the results, SEM observes the acicular-structured crystals filling capillary pores for mortar substrate of the internal microstructure beneath the concrete surface; meanwhile, XRD and FT-IR showed the main hydration products of CPSM to be C-S-H gel and CaCO₃. Besides, MIP also shows CPSM with the ability to clog capillary pores of mortar substrate; thus, it reduces porosity, and appears to benefit in sealing pores or cracks. The depth of CPSM penetration capability indicated by TGA shows 0-10 mm of sealer layer beneath the concrete surface.

Potential pressurized payloads: Fluid and thermal experiments

NASA Technical Reports Server (NTRS)

Swanson, Theodore D.

1992-01-01

Space Station Freedom (SSF) presents the opportunity to perform long term fluid and thermal experiments in a microgravity environment. This presentation provides perspective on the need for fluids/thermal experimentation in a microgravity environment, addresses previous efforts, identifies possible experiments, and discusses the capabilities of a proposed fluid physics/dynamics test facility. Numerous spacecraft systems use fluids for their operation. Thermal control, propulsion, waste management, and various operational processes are examples of such systems. However, effective ground testing is very difficult. This is because the effect of gravity induced phenomena, such as hydrostatic pressure, buoyant convection, and stratification, overcome such forces as surface tension, diffusion, electric potential, etc., which normally dominate in a microgravity environment. Hence, space experimentation is necessary to develop and validate a new fluid based technology. Two broad types of experiments may be performed on SSF: basic research and applied research. Basic research might include experiments focusing on capillary phenomena (with or without thermal and/or solutal gradients), thermal/solutal convection, phase transitions, and multiphase flow. Representative examples of applied research might include two-phase pressure drop, two-phase flow instabilities, heat transfer coefficients, fluid tank fill/drain, tank slosh dynamics, condensate removal enhancement, and void formation within thermal energy storage materials. In order to better support such fluid/thermal experiments on board SSF, OSSA has developed a conceptual design for a proposed Fluid Physics/Dynamics Facility (FP/DF). The proposed facility consists of one facility rack permanently located on SSF and one experimenter rack which is changed out as needed to support specific experiments. This approach will minimize the on-board integration/deintegration required for specific experiments. The FP/DF will have acceleration/vibration compensation, power and thermal interfaces, computer command/data collection, a video imaging system, and a portable glove box for operations. This facility will allow real-time astronaut interaction with the testing.

Features of lava lake filling and draining and their implications for eruption dynamics

USGS Publications Warehouse

Stovall, W.K.; Houghton, Bruce F.; Harris, A.J.L.; Swanson, D.A.

2009-01-01

Lava lakes experience filling, circulation, and often drainage depending upon the style of activity and location of the vent. Features formed by these processes have proved difficult to document due to dangerous conditions during the eruption, inaccessibility, and destruction of features during lake drainage. Kilauea Iki lava lake, Kilauea, Hawai'i, preserves many such features, because lava ponded in a pre-existing crater adjacent to the vent and eventually filled to the level of, and interacted with, the vent and lava fountains. During repeated episodes, a cyclic pattern of lake filling to above vent level, followed by draining back to vent level, preserved features associated with both filling and draining. Field investigations permit us to describe the characteristic features associated with lava lakes on length scales ranging from centimeters to hundreds of meters in a fashion analogous to descriptions of lava flows. Multiple vertical rinds of lava coating the lake walls formed during filling as the lake deepened and lava solidified against vertical faces. Drainage of the lake resulted in uneven formation of roughly horizontal lava shelves on the lakeward edge of the vertical rinds; the shelves correlate with stable, staggered lake stands. Shelves either formed as broken relict slabs of lake crust that solidified in contact with the wall or by accumulation, accretion, and widening at the lake surface in a dynamic lateral flow regime. Thin, upper lava shelves reflect an initially dynamic environment, in which rapid lake lowering was replaced by slower and more staggered drainage with the formation of thicker, more laterally continuous shelves. At all lava lakes experiencing stages of filling and draining these processes may occur and result in the formation of similar sets of features. ?? Springer-Verlag 2009.

Fluid dynamics following flow shut-off in bottle filling

NASA Astrophysics Data System (ADS)

Thete, Sumeet; Appathurai, Santosh; Gao, Haijing; Basaran, Osman

2012-11-01

Bottle filling is ubiquitous in industry. Examples include filling of bottles with shampoos and cleaners, engine oil and pharmaceuticals. In these examples, fluid flows out of a nozzle to fill bottles in an assembly line. Once the required volume of fluid has flowed out of the nozzle, the flow is shut off. However, an evolving fluid thread or string may remain suspended from the nozzle following flow shut-off and persist. This stringing phenomenon can be detrimental to a bottle filling operation because it can adversely affect line speed and filling accuracy by causing uncertainty in fill volume, product loss and undesirable marring of the bottles' exterior surfaces. The dynamics of stringing are studied numerically primarily by using the 1D, slender-jet approximation of the flow equations. A novel feature entails development and use of a new boundary condition downstream of the nozzle exit to expedite the computations. While the emphasis is on stringing of Newtonian fluids and use of 1D approximations, results will also be presented for situations where (a) the fluids are non-Newtonian and (b) the full set of equations are solved without invoking the 1D approximation. Phase diagrams will be presented that identify conditions for which stringing can be problematic.

Soil water retention and maximum capillary drive from saturation to oven dryness

USGS Publications Warehouse

Morel-Seytoux, Hubert J.; Nimmo, John R.

1999-01-01

This paper provides an alternative method to describe the water retention curve over a range of water contents from saturation to oven dryness. It makes two modifications to the standard Brooks and Corey [1964] (B-C) description, one at each end of the suction range. One expression proposed by Rossi and Nimmo [1994] is used in the high-suction range to a zero residual water content. (This Rossi-Nimmo modification to the Brooks-Corey model provides a more realistic description of the retention curve at low water contents.) Near zero suction the second modification eliminates the region where there is a change in suction with no change in water content. Tests on seven soil data sets, using three distinct analytical expressions for the high-, medium-, and low-suction ranges, show that the experimental water retention curves are well fitted by this composite procedure. The high-suction range of saturation contributes little to the maximum capillary drive, defined with a good approximation for a soil water and air system as HcM = ∫0∞ Krwdhc , where krw is relative permeability (or conductivity) to water and hc is capillary suction, a positive quantity in unsaturated soils. As a result, the modification suggested to describe the high-suction range does not significantly affect the equivalence between Brooks-Corey (B-C) and van Genuchten [1980] parameters presented earlier. However, the shape of the retention curve near “natural saturation” has a significant impact on the value of the capillary drive. The estimate using the Brooks-Corey power law, extended to zero suction, will exceed that obtained with the new procedure by 25 to 30%. It is not possible to tell which procedure is appropriate. Tests on another data set, for which relative conductivity data are available, support the view of the authors that measurements of a retention curve coupled with a speculative curve of relative permeability as from a capillary model are not sufficient to accurately determine the (maximum) capillary drive. The capillary drive is a dynamic scalar, whereas the retention curve is of a static character. Only measurements of infiltration rates with time can determine the capillary drive with precision for a given soil.

On the kinetics of the capillary imbibition of a simple fluid through a designed nanochannel using the molecular dynamics simulation approach.

PubMed

Ahadian, Samad; Mizuseki, Hiroshi; Kawazoe, Yoshiyuki

2010-12-15

A molecular dynamics (MD) approach was employed to simulate the imbibition of a designed nanopore by a simple fluid (i.e., a Lennard-Jones (LJ) fluid). The length of imbibition as a function of time for various interactions between the LJ fluid and the pore wall was recorded for this system (i.e., the LJ fluid and the nanopore). By and large, the kinetics of imbibition was successfully described by the Lucas-Washburn (LW) equation, although deviation from it was observed in some cases. This lack of agreement is due to the neglect of the dynamic contact angle (DCA) in the LW equation. Two commonly used models (i.e., hydrodynamic and molecular-kinetic (MK) models) were thus employed to calculate the DCA. It is demonstrated that the MK model is able to justify the simulation results in which are not in good agreement with the simple LW equation. However, the hydrodynamic model is not capable of doing that. Further investigation of the MD simulation data revealed an interesting fact that there is a direct relationship between the wall-fluid interaction and the speed of the capillary imbibition. More evidence to support this claim is presented. Copyright © 2010 Elsevier Inc. All rights reserved.

Modeling meniscus rise in capillary tubes using fluid in rigid-body motion approach

NASA Astrophysics Data System (ADS)

Hamdan, Mohammad O.; Abu-Nabah, Bassam A.

2018-04-01

In this study, a new term representing net flux rate of linear momentum is introduced to Lucas-Washburn equation. Following a fluid in rigid-body motion in modeling the meniscus rise in vertical capillary tubes transforms the nonlinear Lucas-Washburn equation to a linear mass-spring-damper system. The linear nature of mass-spring-damper system with constant coefficients offers a nondimensional analytical solution where meniscus dynamics are dictated by two parameters, namely the system damping ratio and its natural frequency. This connects the numerous fluid-surface interaction physical and geometrical properties to rather two nondimensional parameters, which capture the underlying physics of meniscus dynamics in three distinct cases, namely overdamped, critically damped, and underdamped systems. Based on experimental data available in the literature and the understanding meniscus dynamics, the proposed model brings a new approach of understanding the system initial conditions. Accordingly, a closed form relation is produced for the imbibition velocity, which equals half of the Bosanquet velocity divided by the damping ratio. The proposed general analytical model is ideal for overdamped and critically damped systems. While for underdamped systems, the solution shows fair agreement with experimental measurements once the effective viscosity is determined. Moreover, the presented model shows meniscus oscillations around equilibrium height occur if the damping ratio is less than one.

Analysis of enantiomeric and non-enantiomeric monoterpenes in plant emissions using portable dynamic air sampling/solid-phase microextraction (PDAS-SPME) and chiral gas chromatography/mass spectrometry

NASA Astrophysics Data System (ADS)

Yassaa, Noureddine; Williams, Jonathan

A portable dynamic air sampler (PDAS) using a porous polymer solid-phase microextraction (SPME) fibre has been validated for the determination of biogenic enantiomeric and non-enantiomeric monoterpenes in air. These compounds were adsorbed in the field, and then thermally desorbed at 250 °C in a gas chromatograph injector port connected via a β-cyclodextrin capillary separating column to a mass spectrometer. The optimized method has been applied for investigating the emissions of enantiomeric monoterpenes from Pseudotsuga menziesii (Douglas-fir), Rosmarinus officinalis (Rosemary) and Lavandula lanata (Lavender) which were selected as representative of coniferous trees and aromatic plants, respectively. The enantiomers of α-pinene, sabinene, camphene, δ-3-carene, β-pinene, limonene, β-phellandrene, 4-carene and camphor were successfully determined in the emissions from the three plants. While Douglas-fir showed a strong predominance toward (-)-enantiomers, Rosemary and Lavender demonstrated a large variation in enantiomeric distribution of monoterpenes. The simplicity, rapidity and sensitivity of dynamic sampling with porous polymer coated SPME fibres coupled to chiral capillary gas chromatography/mass spectrometry (GC/MS) makes this method potentially useful for in-field investigations of atmosphere-biosphere interactions and studies of optically explicit atmospheric chemistry.

Altering Emulsion Stability with Heterogeneous Surface Wettability

NASA Astrophysics Data System (ADS)

Meng, Qiang; Zhang, Yali; Li, Jiang; Lammertink, Rob G. H.; Chen, Haosheng; Tsai, Peichun Amy

2016-06-01

Emulsions-liquid droplets dispersed in another immiscible liquid-are widely used in a broad spectrum of applications, including food, personal care, agrochemical, and pharmaceutical products. Emulsions are also commonly present in natural crude oil, hampering the production and quality of petroleum fuels. The stability of emulsions plays a crucial role in their applications, but controlling the stability without external driving forces has been proven to be difficult. Here we show how heterogeneous surface wettability can alter the stability and dynamics of oil-in-water emulsions, generated by a co-flow microfluidic device. We designed a useful methodology that can modify a micro-capillary of desired heterogeneous wettability (e.g., alternating hydrophilic and hydrophobic regions) without changing the hydraulic diameter. We subsequently investigated the effects of flow rates and heterogeneous wettability on the emulsion morphology and motion. The experimental data revealed a universal critical timescale of advective emulsions, above which the microfluidic emulsions remain stable and intact, whereas below they become adhesive or inverse. A simple theoretical model based on a force balance can be used to explain this critical transition of emulsion dynamics, depending on the droplet size and the Capillary number-the ratio of viscous to surface effects. These results give insight into how to control the stability and dynamics of emulsions in microfluidics with flow velocity and different wettability.

Carbon monoxide transport and actions in blood and tissues.

PubMed

Thom, Stephen R

2011-01-01

This chapter will discuss the transport of carbon monoxide (CO) from the environment to the tissues of the body and physiological effects on blood-borne cells and perivascular tissues. It will review the physiology of CO exchange between alveolar gas and pulmonary capillary blood, dynamics of hemoglobin transport, the effects of CO on blood elements, and the effects of CO on extravascular tissues at the capillary bed. Effects of CO from exogenous and endogenous sources on the activities of different proteins will be reviewed. Because CO binds competitively to heme-containing proteins its effects depend on CO concentration relative to alternative ligands. Therefore, some discussion is devoted to how nitric oxide and hydrogen sulfide influence CO effects. © 2011 American Physiological Society.

Experimental Investigation on the Resonance of a Liquid Column in a Capillary Tube.

PubMed

Hilpert; Miller

1999-11-01

Using a visualization technique, we observed the resonance of a water column trapped in a vertically oriented capillary tube due to acoustic excitation. The analysis of the quasi-static response suggests that the upper nonvisible meniscus followed the imposed flow by means of a sliding contact line without changing its shape. We compared the experiments with a previously developed theoretical model that addresses dissipation by assuming an axially symmetric and incompressible flow field that is spatially constant along the tube axis. Whereas the model agrees well with the measured quasi-static response, the deviations in the dynamic response reveal shortcomings of the model due to the simplified treatment of the viscous dissipation. Copyright 1999 Academic Press.

High-throughput biological small-angle X-ray scattering with a robotically loaded capillary cell

PubMed Central

Nielsen, S. S.; Møller, M.; Gillilan, R. E.

2012-01-01

With the rise in popularity of biological small-angle X-ray scattering (BioSAXS) measurements, synchrotron beamlines are confronted with an ever-increasing number of samples from a wide range of solution conditions. To meet these demands, an increasing number of beamlines worldwide have begun to provide automated liquid-handling systems for sample loading. This article presents an automated sample-loading system for BioSAXS beamlines, which combines single-channel disposable-tip pipetting with a vacuum-enclosed temperature-controlled capillary flow cell. The design incorporates an easily changeable capillary to reduce the incidence of X-ray window fouling and cross contamination. Both the robot-control and the data-processing systems are written in Python. The data-processing code, RAW, has been enhanced with several new features to form a user-friendly BioSAXS pipeline for the robot. The flow cell also supports efficient manual loading and sample recovery. An effective rinse protocol for the sample cell is developed and tested. Fluid dynamics within the sample capillary reveals a vortex ring pattern of circulation that redistributes radiation-damaged material. Radiation damage is most severe in the boundary layer near the capillary surface. At typical flow speeds, capillaries below 2 mm in diameter are beginning to enter the Stokes (creeping flow) regime in which mixing due to oscillation is limited. Analysis within this regime shows that single-pass exposure and multiple-pass exposure of a sample plug are functionally the same with regard to exposed volume when plug motion reversal is slow. The robot was tested on three different beamlines at the Cornell High-Energy Synchrotron Source, with a variety of detectors and beam characteristics, and it has been used successfully in several published studies as well as in two introductory short courses on basic BioSAXS methods. PMID:22509071

Expanding Taylor bubble under constant heat flux

NASA Astrophysics Data System (ADS)

Voirand, Antoine; Benselama, Adel M.; Ayel, Vincent; Bertin, Yves

2016-09-01

Modelization of non-isothermal bubbles expanding in a capillary, as a contribution to the understanding of the physical phenomena taking place in Pulsating Heat Pipes (PHPs), is the scope of this paper. The liquid film problem is simplified and solved, while the thermal problem takes into account a constant heat flux density applied at the capillary tube wall, exchanging with the liquid film surrounding the bubble and also with the capillary tube outside medium. The liquid slug dynamics is solved using the Lucas-Washburn equation. Mass and energy balance on the vapor phase allow governing equations of bubble expansion to be written. The liquid and vapor phases are coupled only through the saturation temperature associated with the vapor pressure, assumed to be uniform throughout the bubble. Results show an over-heating of the vapor phase, although the particular thermal boundary condition used here always ensures an evaporative mass flux at the liquid-vapor interface. Global heat exchange is also investigated, showing a strong decreasing of the PHP performance to convey heat by phase change means for large meniscus velocities.

A mean curvature model for capillary flows in asymmetric containers and conduits

NASA Astrophysics Data System (ADS)

Chen, Yongkang; Tavan, Noël; Weislogel, Mark M.

2012-08-01

Capillarity-driven flows resulting from critical geometric wetting criterion are observed to yield significant shifts of the bulk fluid from one side of the container to the other during "zero gravity" experiments. For wetting fluids, such bulk shift flows consist of advancing and receding menisci sometimes separated by secondary capillary flows such as rivulet-like flows along gaps. Here we study the mean curvature of an advancing meniscus in hopes of approximating a critical boundary condition for fluid dynamics solutions. It is found that the bulk shift flows behave as if the bulk menisci are either "connected" or "disconnected." For the connected case, an analytic method is developed to calculate the mean curvature of the advancing meniscus in an asymptotic sense. In contrast, for the disconnected case the method to calculate the mean curvature of the advancing and receding menisci uses a well-established procedure. Both disconnected and connected bulk shifts can occur as the first tier flow of more complex compound capillary flows. Preliminary comparisons between the analytic method and the results of drop tower experiments are encouraging.

Anti-infiltration for fabrication of a suspended nanoparticle layer on porous close-packed colloidal arrays.

PubMed

Teh, Lay K; Yan, Qingfeng; Wong, Chee C

2009-04-01

We develop a new method to fabricate suspended sheets of nanocrystals (NCs) on porous surfaces. The method relies on the resistance of an aqueous suspension droplet to infiltrate a porous network; hence, the method is named anti-infiltration. The process works by combining fluid dynamics of a liquid droplet during impact/absorption onto a porous surface with the convective self-assembly of NCs. The immobilization of the liquid droplet edge due to the self-assembly of NCs at the meniscus is harnessed to halt the lateral spreading of the droplet and, consequently, the capillary penetration of the liquid immediately after droplet impact. Further capillary penetration of the liquid is drastically reduced because of the competition between capillary forces and convective losses as well as the rapid occlusion of the pores as soon as a continuous NC film has formed upon evaporation of the suspension. This method holds promise for a wide variety of optoelectronic, sensing, and separation membrane applications. As an example, we demonstrate that these suspended NC layers are suitable candidates as planar defects embedded within a colloidal photonic crystal.

Separation of attogram terpenes by the capillary zone electrophoresis with fluorometric detection.

PubMed

Kubesová, Anna; Horká, Marie; Růžička, Filip; Slais, Karel; Glatz, Zdeněk

2010-11-12

An original method based on capillary zone electrophoresis with fluorimetric detection has been developed for the determination of terpenic compounds. The method is based on the separation of a terpenes dynamically labeled by the non-ionogenic tenside poly(ethylene glycol) pyrenebutanoate, which was used previously for the labeling of biopolymers. The background electrolytes were composed of taurine-Tris buffer (pH 8.4). In addition to the non-ionogenic tenside aceton and poly(ethylene glycol) were used as the additives. The capillary zone electrophoresis with fluorometric detection at the excitation wavelength 335 nm and the emission wavelength 463 nm was successfully applied to the analysis of tonalid, cholesterol, vitamin A, ergosterol, estrone and farnesol at level of 10(-17) mol L(-1). Farnesol, is produced by Candida albicans as an extracellular quorum-sensing molecule that influences expression of a number of virulence factors, especially morphogenesis and biofilm formation. It enables this yeast to cause serious nosocomial infections. The sensitivity of this method was demonstrated on the separation of farnesol directly from the cultivation medium. Copyright © 2010 Elsevier B.V. All rights reserved.

Capillary Ion Concentration Polarization for Power-Free Salt Purification

NASA Astrophysics Data System (ADS)

Park, Sungmin; Jung, Yeonsu; Cho, Inhee; Kim, Ho-Young; Kim, Sung Jae

2014-11-01

In this presentation, we experimentally and theoretically demonstrated the capillary based ion concentration polarization for power-free salt purification system. Traditional ion concentration polarization phenomenon has been studied for a decade for both fundamental nanoscale fluid dynamics and novel engineering applications such as desalination, preconcentration and energy harvesting devices. While the conventional system utilizes an external power source, the system based on capillary ion concentration polarization is capable of perm-selective ion transportation only by capillarity so that the same ion depletion zone can be formed without any external power sources. An ion concentration profile near the nanostructure was tracked using fluorescent probes and analyzed by solving the modified Nernst-Planck equation. As a result, the concentration in the vicinity of the nanostructure was at least 10 times lower than that of bulk electrolyte and thus, the liquid absorbed into the nanostructure had the low concentration. This mechanism can be used for the power free salt purification system which would be significantly useful in underdeveloped and remote area. This work was supported by Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-MA1301-02.

Interfacial free energy of the NaCl crystal-melt interface from capillary wave fluctuations.

PubMed

Benet, Jorge; MacDowell, Luis G; Sanz, Eduardo

2015-04-07

In this work we study, by means of molecular dynamics simulations, the solid-liquid interface of NaCl under coexistence conditions. By analysing capillary waves, we obtain the stiffness for different orientations of the solid and calculate the interfacial free energy by expanding the dependency of the interfacial free energy with the solid orientation in terms of cubic harmonics. We obtain an average value for the solid-fluid interfacial free energy of 89 ± 6 mN m(-1) that is consistent with previous results based on the measure of nucleation free energy barriers [Valeriani et al., J. Chem. Phys. 122, 194501 (2005)]. We analyse the influence of the simulation setup on interfacial properties and find that facets prepared as an elongated rectangular stripe give the same results as those prepared as squares for all cases but the 111 face. For some crystal orientations, we observe at small wave-vectors a behaviour not consistent with capillary wave theory and show that this behavior does not depend on the simulation setup.

Scaling during capillary thinning of particle-laden drops

NASA Astrophysics Data System (ADS)

Thete, Sumeet; Wagoner, Brayden; Basaran, Osman

2017-11-01

A fundamental understanding of drop formation is crucial in many applications such as ink-jet printing, microfluidic devices, and atomization. During drop formation, the about-to-form drop is connected to the fluid hanging from the nozzle via a thinning filament. Therefore, the physics of capillary thinning of filaments is key to understanding drop formation and has been thoroughly studied for pure Newtonian fluids using theory, simulations, and experiments. In some of the applications however, the forming drop and hence the thinning filament may contain solid particles. The thinning dynamics of such particle-laden filaments differs radically from that of particle-free filaments. Moreover, our understanding of filament thinning in the former case is poor compared to that in the latter case despite the growing interest in pinch-off of particle-laden filaments. In this work, we go beyond similar studies and experimentally explore the impact of solid particles on filament thinning by measuring both the radial and axial scalings in the neck region. The results are summarized in terms of a phase diagram of capillary thinning of particle-laden filaments.

[Pathomorphological peculiarities of hemomicrocirculatory bed of the small and large intestine in acute peritonitis].

PubMed

Siplivyĭ, V A; Grinchenko, S V; Gorgol', N I; Dotsenko, V V; Evtushenko, A V

2014-01-01

Experimental comparative morphological investigation of hemomicrocirculation bed (HMCB) of the small and large bowel wall was performed in dynamics of an acute serous peritonitis. Spreaded aseptic peritonitis was simulated using injection of 5 ml of gamma-caraginen (Sigma, USA) in 1 ml of isotonic solution of sodium chloride. On the early stage of peritonitis (in 12 h from beginning of the experiment) in mucosa of small bowel nonsignificant venuls dilatation and the capillary lumen reduction were observed. In 1 day (reactive stage of peritonitis) in mucosa the quantity of capillars have had reduced significantly, comparing with such observed previously. On the 2-nd day (toxic stage of peritonitis) some capillary dilatation in intestinal villi and crypts coexistant with the blood rheology disorders in a form of stasis, change in permeability of the vessels walls, predominantly of the venous, was noted. On the 3-d day (late stage) the arteriol's spasm have had reduced, capillary paralytic dilatation was revealed. The staged course of experimental peritonitis with the HMCB changes, characteristic for every stage, was confirmed, basing on analysis of the investigation result.

Two reference time scales for studying the dynamic cavitation of liquid films

NASA Technical Reports Server (NTRS)

Sun, D. C.; Brewe, David E.

1991-01-01

Two formulas, one for characteristic time of filling a void with a vapor of the surrounding liquid, and one of filling the void by diffusion of the dissolved gas in the liquid, are derived. Based on this analysis, it is seen that in an oil film bearing operating under dynamic loads, the content of cavitation region should be oil vapor rather than the air liberated from solution, if the oil is free of entrained air.

Capillary fluctuations of surface steps: An atomistic simulation study for the model Cu(111) system

NASA Astrophysics Data System (ADS)

Freitas, Rodrigo; Frolov, Timofey; Asta, Mark

2017-10-01

Molecular dynamics (MD) simulations are employed to investigate the capillary fluctuations of steps on the surface of a model metal system. The fluctuation spectrum, characterized by the wave number (k ) dependence of the mean squared capillary-wave amplitudes and associated relaxation times, is calculated for 〈110 〉 and 〈112 〉 steps on the {111 } surface of elemental copper near the melting temperature of the classical potential model considered. Step stiffnesses are derived from the MD results, yielding values from the largest system sizes of (37 ±1 ) meV/A ˚ for the different line orientations, implying that the stiffness is isotropic within the statistical precision of the calculations. The fluctuation lifetimes are found to vary by approximately four orders of magnitude over the range of wave numbers investigated, displaying a k dependence consistent with kinetics governed by step-edge mediated diffusion. The values for step stiffness derived from these simulations are compared to step free energies for the same system and temperature obtained in a recent MD-based thermodynamic-integration (TI) study [Freitas, Frolov, and Asta, Phys. Rev. B 95, 155444 (2017), 10.1103/PhysRevB.95.155444]. Results from the capillary-fluctuation analysis and TI calculations yield statistically significant differences that are discussed within the framework of statistical-mechanical theories for configurational contributions to step free energies.