33 CFR 104.275 - Security measures for handling cargo.

Code of Federal Regulations, 2010 CFR

2010-07-01

... documentation, or that cargo markings or container numbers match the information provided with shipping..., container, or other cargo transport units are loaded; (3) Intensifying screening of vehicles to be loaded on... cargo. 104.275 Section 104.275 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND...

33 CFR 104.275 - Security measures for handling cargo.

Code of Federal Regulations, 2014 CFR

2014-07-01

... documentation, or that cargo markings or container numbers match the information provided with shipping..., container, or other cargo transport units are loaded; (3) Intensifying screening of vehicles to be loaded on... cargo. 104.275 Section 104.275 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND...

Use of a parasitic wasp as a biosensor

USDA-ARS?s Scientific Manuscript database

Screening cargo for illicit substances is still in need of high-throughput inspection systems that can rapidly screen and accurately identify suspicious cargo. Here we investigate the ability of a parasitic wasp, Microplitis croceipes to detect and respond to methyl benzoate, the volatile component ...

77 FR 65395 - Air Cargo Advance Screening (ACAS) Pilot Program

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-26

... DEPARTMENT OF HOMELAND SECURITY U.S. Customs and Border Protection Air Cargo Advance Screening (ACAS) Pilot Program Correction In notice document 2012-26031 appearing on pages 65006-65009 in the issue of October 24, 2012 make the following correction: On page 65007, in the first column, under the...

49 CFR 1515.9 - Appeal of security threat assessment based on other analyses.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., foreign air carriers, indirect air carriers (IACs), certified cargo screening facilities, or validation...'s reply and any accompanying information, and any other materials or information available to him or... operators, foreign air carriers, IACs, certified cargo screening facilities, or validation firms. [72 FR...

Aviation security : preliminary observations on TSA's progress and challenges in meeting the statutory mandate for screening air cargo on passenger aircraft.

DOT National Transportation Integrated Search

2009-03-01

The Implementing : Recommendations of the 9/11 : Commission Act of 2007 mandates : the Department of Homeland : Security (DHS) to establish a : system to physically screen 50 : percent of cargo transported on : passenger aircraft by February : 2009 a...

77 FR 25187 - Extension of Agency Information Collection Activity Under OMB Review: Certified Cargo Screening...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-27

...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0053, abstracted below to OMB for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on February 24, 2012, 77 FR 11146, and TSA received no comments. The collections include: (1) Applications from entities that wish to become Certified Cargo Screening Facilities (CCSFs); (2) personal information to allow TSA to conduct security threat assessments on key individuals employed by the CCSFs; (3) implementation of a standard security program or submission of a proposed modified security program; (4) information on the amount of cargo screened; (5) recordkeeping requirements for CCSFs, and any other requests for information relating to cargo screening required to meet the Implementing Recommendations of the 9/11 Commission Act of 2007 (9/ 11 Act) and the Aviation and Transportation Security Act (ATSA) mandates. TSA is seeking the renewal of the ICR for the continuation of the program in order to secure passenger aircraft transporting cargo as required in the 9/11 Act.

77 FR 65006 - Air Cargo Advance Screening (ACAS) Pilot Program

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-24

... later than the time of departure of the aircraft for the United States (from specified locations) or... earliest point practicable prior to loading of the cargo onto the aircraft destined to or transiting... electronic cargo information by way of a CBP-approved electronic data interchange (EDI) system before the...

Flashback flame arrester devices for fuel cargo tank vapor vents

NASA Technical Reports Server (NTRS)

Bjorklund, R. A.; Kushida, R. O.

1981-01-01

The flame quenching capability of four types of flame arresting devices suitable for installation on fuel cargo tank vents of marine transport vessels is evaluated. A single 30 mesh screen, a dual 20 mesh screen, a spiral wound crimped metal ribbon, and a packed bed of ballast rings were tested. Flame speed and flame penetration of the test arresters were determined. Eight fuels representative of bulk cargoes were tested. The test arresters quenched a minimum of three flashback flames from all eight fuels, with one exception: high speed ethylene flames penetrated the dual 20 mesh screen on three tests. The arresters withstood the sustained flame from a propane/air mixture for 30 minutes. None of the arresters withstood the sustained flame from an ethylene/air mixture for more than 7 minutes.

49 CFR 1546.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2013 CFR

2013-10-01

... thoroughly manipulate and handle such baggage, containers, cargo, and other objects subject to screening. (5... operating screening equipment must be able to distinguish on the screening equipment monitor the appropriate...

49 CFR 1546.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2011 CFR

2011-10-01

... thoroughly manipulate and handle such baggage, containers, cargo, and other objects subject to screening. (5... operating screening equipment must be able to distinguish on the screening equipment monitor the appropriate...

49 CFR 1546.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2012 CFR

2012-10-01

... thoroughly manipulate and handle such baggage, containers, cargo, and other objects subject to screening. (5... operating screening equipment must be able to distinguish on the screening equipment monitor the appropriate...

49 CFR 1546.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2010 CFR

2010-10-01

... thoroughly manipulate and handle such baggage, containers, cargo, and other objects subject to screening. (5... operating screening equipment must be able to distinguish on the screening equipment monitor the appropriate...

49 CFR 1546.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2014 CFR

2014-10-01

... thoroughly manipulate and handle such baggage, containers, cargo, and other objects subject to screening. (5... operating screening equipment must be able to distinguish on the screening equipment monitor the appropriate...

Multi-energy x-ray detectors to improve air-cargo security

NASA Astrophysics Data System (ADS)

Paulus, Caroline; Moulin, Vincent; Perion, Didier; Radisson, Patrick; Verger, Loïck

2017-05-01

X-ray based systems have been used for decades to screen luggage or cargo to detect illicit material. The advent of energy-sensitive photon-counting x-ray detectors mainly based on Cd(Zn)Te semi-conductor technology enables to improve discrimination between materials compared to single or dual energy technology. The presented work is part of the EUROSKY European project to develop a Single European Secure Air-Cargo Space. "Cargo" context implies the presence of relatively heavy objects and with potentially high atomic number. All the study is conducted on simulations with three different detectors: a typical dual energy sandwich detector, a realistic model of the commercial ME100 multi-energy detector marketed by MULTIX, and a ME100 "Cargo": a not yet existing modified multi-energy version of the ME100 more suited to air freight cargo inspection. Firstly, a comparison on simulated measurements shows the performances improvement of the new multi-energy detectors compared to the current dual-energy one. The relative performances are evaluated according to different criteria of separability or contrast-to-noise ratio and the impact of different parameters is studied (influence of channel number, type of materials and tube voltage). Secondly, performances of multi-energy detectors for overlaps processing in a dual-view system is accessed: the case of orthogonal projections has been studied, one giving dimensional values, the other one providing spectral data to assess effective atomic number. A method of overlap correction has been proposed and extended to multi-layer objects case. Therefore, Calibration and processing based on bi-material decomposition have been adapted for this purpose.

National Security Science and Technology Initiative: Air Cargo Screening, Final Report for CRADA Number NFE-07-01081

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bingham, Philip; Bush, John; Bowerman, Biays

The non-intrusive inspection (NII) of consolidated air cargo carried on commercial passenger aircraft continues to be a technically challenging, high-priority requirement of the Department of Homeland Security’s Science and Technology Directorate (DHS S&T), the Transportation Security Agency and the Federal Aviation Administration. The goal of deploying a screening system that can reliably and cost-effectively detect explosive threats in consolidated cargo without adversely affecting the flow of commerce will require significant technical advances that will take years to develop. To address this critical National Security need, the Battelle Memorial Institute (Battelle), under a Cooperative Research and Development Agreement (CRADA) with fourmore » of its associated US Department of Energy (DOE) National Laboratories (Oak Ridge, Pacific Northwest, Idaho, and Brookhaven), conducted a research and development initiative focused on identifying, evaluating, and integrating technologies for screening consolidated air cargo for the presence of explosive threats. Battelle invested $8.5M of internal research and development funds during fiscal years 2007 through 2009.« less

Radiation and Shipping Port Security | RadTown USA | US ...

EPA Pesticide Factsheets

2017-08-07

Cargo comes into the U.S. from all over the world. Ports can ensure safety by: Screening cargo for radiation before it gets here. Safely handling any radioactive material that needs to pass through a port.

Detection of weapons of mass destruction

NASA Astrophysics Data System (ADS)

Bjorkholm, Paul J.

2003-07-01

High Energy X-ray cargo screening is a mature technology that has proven its value in the detection of contraband material hidden within cargo including fully loaded sea containers. To date high energy screening has been largely applied to manifest verification and to drug detection. However, the dramatic change in world terrorism has altered the application. Now it is essential that weapons of mass destruction (WMD"s) be interdicted with incredibly high accuracy. The implication of a missed detection has gone from loss of revenue or the lowering of the street price of drugs to potentially stopping, at least for some significant time, most world commerce. Screening containers with high energy x-rays (~250+ mm of steel penetration) is capable of detecting all nuclear threats at a fraction of the strategically important mass. The screening operation can be automated so that no human decisions are required with very low false alarms. Finally, the goal of 100% inspection of cargo inbound to the United States from the twenty largest international ports is an achievable goal with hardware costs in the area of that already spent on airport security.

National Security Science and Technology Initiative: Air Cargo Screening

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bingham, Philip R; White, Tim; Cespedes, Ernesto

The non-intrusive inspection (NII) of consolidated air cargo carried on commercial passenger aircraft continues to be a technically challenging, high-priority requirement of the Department of Homeland Security's Science and Technology Directorate (DHS S&T), the Transportation Security Agency and the Federal Aviation Administration. The goal of deploying a screening system that can reliably and cost-effectively detect explosive threats in consolidated cargo without adversely affecting the flow of commerce will require significant technical advances that will take years to develop. To address this critical National Security need, the Battelle Memorial Institute (Battelle), under a Cooperative Research and Development Agreement (CRADA) with fourmore » of its associated US Department of Energy (DOE) National Laboratories (Oak Ridge, Pacific Northwest, Idaho, and Brookhaven), conducted a research and development initiative focused on identifying, evaluating, and integrating technologies for screening consolidated air cargo for the presence of explosive threats. Battelle invested $8.5M of internal research and development funds during fiscal years 2007 through 2009. The primary results of this effort are described in this document and can be summarized as follows: (1) Completed a gap analysis that identified threat signatures and observables, candidate technologies for detection, their current state of development, and provided recommendations for improvements to meet air cargo screening requirements. (2) Defined a Commodity/Threat/Detection matrix that focuses modeling and experimental efforts, identifies technology gaps and game-changing opportunities, and provides a means of summarizing current and emerging capabilities. (3) Defined key properties (e.g., elemental composition, average density, effective atomic weight) for basic commodity and explosive benchmarks, developed virtual models of the physical distributions (pallets) of three commodity types and three explosive benchmarks combinations, and conducted modeling and simulation studies to begin populating the matrix of commodities, threats, and detection technologies. (4) Designed and fabricated basic (homogeneous) commodity test pallets and fabricated inert stimulants to support experiments and to validate modeling/simulation results. (5) Developed/expanded the team's capabilities to conduct full-scale imaging (neutron and x-ray) experiments of air cargo commodities and explosive benchmarks. (6) Conducted experiments to improve the collection of trace particles of explosives from a variety of surfaces representative of air cargo materials by means of mechanical (air/vibration/pressure), thermal, and electrostatic methods. Air cargo screening is a difficult challenge that will require significant investment in both research and development to find a suitable solution to ensure the safety of passengers without significantly hindering the flow of commodities. The initiative funded by Battelle has positioned this group to make major contributions in meeting the air cargo challenge by developing collaborations, developing laboratory test systems, improving knowledge of the challenges (both technical and business) for air cargo screening, and increasing the understanding of the capabilities for current inspection methods (x-ray radiography, x-ray backscatter, etc.) and potential future inspection methods (neutron radiography, fusion of detector modalities, advanced trace detection, etc.). Lastly, air cargo screening is still an issue that will benefit from collaboration between Department of Energy Laboratories and Battelle. On January 7, 2010, DHS Secretary Napolitano joined White House Press Secretary Robert Gibbs and Assistant to the President for Counterterrorism and Homeland Security John Brennan to announce several recommendations DHS has made to the President for improving the technology and procedures used to protect air travel from acts of terrorism. (This announcement followed the 25 Dec'09 Delta/Northwest Airlines Flight 253 terror attack.) Secretary Napolitano outlined five recommendations DHS will pursue to enhance the safety of the traveling public. One of the five recommendations, read as follows: 'Establish a partnership on aviation security between DHS and the Department of Energy and its National Laboratories in order to develop new and more effective technologies to deter and disrupt known threats and proactively anticipate and protect against new ways by which terrorists could seek to board an aircraft.' In conclusion, it appears very timely that Battelle and its DOE lab partners initiated a serious collaboration on the air cargo topic, and that we should continue to work toward future collaboration in response to the government's needs.« less

Aviation Security: Background and Policy Options for Screening and Securing Air Cargo

DTIC Science & Technology

2008-02-25

today, employees with unescorted access privileges to security identification display areas ( SIDAs ) may access secured areas and aircraft without...where all-cargo aircraft are loaded and unloaded, as security identification display areas ( SIDAs ). This effectively elevates the required security

75 FR 9915 - Extension of Agency Information Collection Activity Under OMB Review: Certified Cargo Screening...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-04

...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), OMB control number 1652-0053, abstracted below to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on November 16, 2009, 74 FR 58967. TSA has received no comments. The collections include: (1) Applications from entities that wish to become Certified Cargo Screening Facilities (CCSF) or operate as a TSA-approved validation firm; (2) personal information to allow TSA to conduct security threat assessments on key individuals employed by the CCSFs and validation firms; (3) implementation of a standard security program or submission of a proposed modified security program; (4) information on the amount of cargo screened; (5) recordkeeping requirements for CCSFs and validation firms; and (6) submission of validation reports to TSA. TSA is seeking the renewal of the ICR for the continuation of the program in order to secure passenger aircraft carrying cargo by the deadlines set out in the Implementing Recommendations of the 9/11 Commission Act of 2007.

49 CFR 1544.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2010 CFR

2010-10-01

... must be able to efficiently and thoroughly manipulate and handle such baggage, containers, cargo, and... the screening equipment monitor the appropriate imaging standard specified in the aircraft operator's...

49 CFR 1544.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2011 CFR

2011-10-01

... must be able to efficiently and thoroughly manipulate and handle such baggage, containers, cargo, and... the screening equipment monitor the appropriate imaging standard specified in the aircraft operator's...

49 CFR 1544.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2014 CFR

2014-10-01

... must be able to efficiently and thoroughly manipulate and handle such baggage, containers, cargo, and... the screening equipment monitor the appropriate imaging standard specified in the aircraft operator's...

49 CFR 1544.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2012 CFR

2012-10-01

... must be able to efficiently and thoroughly manipulate and handle such baggage, containers, cargo, and... the screening equipment monitor the appropriate imaging standard specified in the aircraft operator's...

49 CFR 1544.405 - Qualifications of screening personnel.

Code of Federal Regulations, 2013 CFR

2013-10-01

... must be able to efficiently and thoroughly manipulate and handle such baggage, containers, cargo, and... the screening equipment monitor the appropriate imaging standard specified in the aircraft operator's...

Scatter in Cargo Radiography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Erin A. Miller; Joseph A. Caggiano; Robert C. Runkle

As a complement to passive detection systems, radiographic inspection of cargo is an increasingly important tool for homeland security because it has the potential to detect highly attenuating objects associated with special nuclear material or surrounding shielding, in addition to screening for items such as drugs or contraband. Radiographic detection of such threat objects relies on high image contrast between regions of different density and atomic number (Z). Threat detection is affected by scatter of the interrogating beamin the cargo, the radiographic system itself, and the surrounding environment, which degrades image contrast. Here, we estimate the extent to which scattermore » plays a role in radiographic imaging of cargo containers. Stochastic transport simulations were performed to determine the details of the radiography equipment and surrounding environment, which are important in reproducing measured data and to investigate scatter magnitudes for typical cargo. We find that scatter plays a stronger role in cargo radiography than in typicalmedical imaging scenarios, even for low-density cargo, with scatter-toprimary ratios ranging from 0.14 for very low density cargo, to between 0.20 and 0.40 for typical cargo, and higher yet for dense cargo.« less

46 CFR 154.500 - Cargo and process piping standards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Equipment Cargo and Process Piping Systems § 154.500 Cargo and process piping standards. The cargo liquid and vapor piping and process piping systems must meet the requirements in §§ 154.503 through 154.562... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo and process piping standards. 154.500 Section 154...

A microscopy-based screen employing multiplex genome sequencing identifies cargo-specific requirements for dynein velocity

PubMed Central

Tan, Kaeling; Roberts, Anthony J.; Chonofsky, Mark; Egan, Martin J.; Reck-Peterson, Samara L.

2014-01-01

The timely delivery of membranous organelles and macromolecules to specific locations within the majority of eukaryotic cells depends on microtubule-based transport. Here we describe a screening method to identify mutations that have a critical effect on intracellular transport and its regulation using mutagenesis, multicolor-fluorescence microscopy, and multiplex genome sequencing. This screen exploits the filamentous fungus Aspergillus nidulans, which has many of the advantages of yeast molecular genetics but uses long-range microtubule-based transport in a manner more similar to metazoan cells. Using this method, we identified seven mutants that represent novel alleles of components of the intracellular transport machinery: specifically, kinesin-1, cytoplasmic dynein, and the dynein regulators Lis1 and dynactin. The two dynein mutations identified in our screen map to dynein's AAA+ catalytic core. Single-molecule studies reveal that both mutations reduce dynein's velocity in vitro. In vivo these mutants severely impair the distribution and velocity of endosomes, a known dynein cargo. In contrast, another dynein cargo, the nucleus, is positioned normally in these mutants. These results reveal that different dynein functions have distinct stringencies for motor performance. PMID:24403603

Detecting terrorist nuclear weapons at sea: The 10th door problem

DOE Office of Scientific and Technical Information (OSTI.GOV)

Slaughter, D R

2008-09-15

While screening commercial cargo containers for the possible presence of WMD is important and necessary smugglers have successfully exploited the many other vehicles transporting cargo into the US including medium and small vessels at sea. These vessels provide a venue that is currently not screened and widely used. Physics limits that make screening of large vessels prohibitive impractical do not prohibit effective screening of the smaller vessels. While passive radiation detection is probably ineffective at sea active interrogation may provide a successful approach. The physics limits of active interrogation of ships at sea from standoff platforms are discussed. Autonomous platformsmore » that could carry interrogation systems at sea, both airborne and submersible, are summarized and their utilization discussed. An R&D program to investigate the limits of this approach to screening ships at sea is indicated and limitations estimated.« less

Meta-expert system for cargo container screening

NASA Astrophysics Data System (ADS)

Alberts, David S.

1994-02-01

This paper reports upon improvements and extensions of rule-based expert systems and related technologies in the context of their application to the cargo container screening problem. These innovations have been incorporated into a system built for and deployed by U.S. Customs with funding provided by the DCI's Counter Narcotics Committee. Given the serious nature of the drug smuggling threat and the low probability of intercept, the ability to target the extremely limited inspectional resources available to U.S. Customs is a prerequisite for success in fighting the `Drug War.'

49 CFR 1544.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) Preventing or deterring the carriage of any explosive or incendiary. Each aircraft operator operating under a... operator operating under a full program or a full all-cargo program, or a twelve-five program in an all... program, before loading it on its aircraft. (c) Control. Each aircraft operator operating under a full...

49 CFR 1549.1 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-10-01

... screening facility to screen cargo that will be transported on a passenger aircraft operated under a full program under 49 CFR 1544.101(a), or a foreign air carrier operating under a program under 49 CFR 1546.101...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Geelhood, Bruce D.; Wogman, Ned A.

In view of the terrorist threats to the United States, the country needs to consider new vectors and weapons related to nuclear and radiological threats against our homeland. The traditional threat vectors, missiles and bombers, have expanded to include threats arriving through the flow of commerce. The new commerce-related vectors include: sea cargo, truck cargo, rail cargo, air cargo, and passenger transport. The types of weapons have also expanded beyond nuclear war-heads to include radiation dispersal devices (RDD) or “dirty” bombs. The consequences of these nuclear and radiological threats are considered. The defense against undesirable materials enter-ing our borders ismore » considered. The radiation and other signatures of potential nuclear and radio-logical threats are examined along with potential sensors to discover undesirable items in the flow of commerce. Techniques to improve detection are considered. A strategy of primary and secondary screening is proposed to rapidly clear most cargo and carefully examine suspect cargo.« less

Nuclear Terrorism: Calibrating Funding for Defensive Programs in Response to the Threat

DTIC Science & Technology

2009-12-01

fertilizer , ceramic tile, and bananas, slow the cargo screening process and in some cases have even led officials to reduce the sensitivity settings...kilograms of HEU or 8 kilograms of plutonium (weights roughly equated to the size of a melon and a plum respectively).234 Terrorists would likely...ed. Schwartz, 214. 69 On February 26, 1993, terrorists detonated 1,400 pounds of fertilizer -based explosives in the underground parking garage of

77 FR 11146 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Certified...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-24

...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), OMB control number 1652-0053, abstracted below that we will submit to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. The collections include: (1) Applications from entities that wish to become Certified Cargo Screening Facilities (CCSF); (2) personal information to allow TSA to conduct security threat assessments on key individuals employed by the CCSFs; (3) acceptance of a standard security program or submission of a proposed modified security program; (4) information on the amount of cargo screened; and (5) recordkeeping requirements for CCSFs. TSA is seeking the renewal of the ICR for the continuation of the program in order to secure passenger aircraft carrying cargo.

75 FR 63192 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Air Cargo...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-14

... programs, security threat assessments (STA), known shipper data via the Known Shipper Management System... baggage, and other articles, that will be carried aboard a passenger aircraft; and (2) to establish a system to screen, inspect, report, or otherwise ensure the security of all cargo that is to be...

46 CFR 154.650 - Cargo tank and process pressure vessel welding.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank and process pressure vessel welding. 154.650... Equipment Construction § 154.650 Cargo tank and process pressure vessel welding. (a) Cargo tank and process pressure vessel welding must meet Subpart 54.05 and Part 57 of this chapter. (b) Welding consumables used...

46 CFR 154.650 - Cargo tank and process pressure vessel welding.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Cargo tank and process pressure vessel welding. 154.650... Equipment Construction § 154.650 Cargo tank and process pressure vessel welding. (a) Cargo tank and process pressure vessel welding must meet Subpart 54.05 and Part 57 of this chapter. (b) Welding consumables used...

46 CFR 154.650 - Cargo tank and process pressure vessel welding.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Cargo tank and process pressure vessel welding. 154.650... Equipment Construction § 154.650 Cargo tank and process pressure vessel welding. (a) Cargo tank and process pressure vessel welding must meet Subpart 54.05 and Part 57 of this chapter. (b) Welding consumables used...

46 CFR 154.650 - Cargo tank and process pressure vessel welding.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Cargo tank and process pressure vessel welding. 154.650... Equipment Construction § 154.650 Cargo tank and process pressure vessel welding. (a) Cargo tank and process pressure vessel welding must meet Subpart 54.05 and Part 57 of this chapter. (b) Welding consumables used...

46 CFR 154.650 - Cargo tank and process pressure vessel welding.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Cargo tank and process pressure vessel welding. 154.650... Equipment Construction § 154.650 Cargo tank and process pressure vessel welding. (a) Cargo tank and process pressure vessel welding must meet Subpart 54.05 and Part 57 of this chapter. (b) Welding consumables used...

77 FR 11145 - Intent to Request Renewal From OMB of One Current Public Collection of Information: Air Cargo...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-24

...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), OMB control number 1652-0040, abstracted below that we will submit to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. This ICR involves five broad categories of affected populations: airports, passenger aircraft operators, foreign air carriers, indirect air carriers operating under a security program, and all-cargo carriers. The collections of information that make up this ICR are security programs, security threat assessments (STA), known shipper data via the Known Shipper Management System (KSMS), Air Cargo Data Management System (ACDMS), Cargo Reporting Tool for cargo screening reporting, and evidence of compliance recordkeeping. TSA seeks continued OMB approval in order to secure passenger aircraft carrying cargo as authorized in the Aviation and Transportation Security Act.

A systematic approach to pair secretory cargo receptors with their cargo suggests a mechanism for cargo selection by Erv14.

PubMed

Herzig, Yonatan; Sharpe, Hayley J; Elbaz, Yael; Munro, Sean; Schuldiner, Maya

2012-01-01

The endoplasmic reticulum (ER) is the site of synthesis of secreted and membrane proteins. To exit the ER, proteins are packaged into COPII vesicles through direct interaction with the COPII coat or aided by specific cargo receptors. Despite the fundamental role of such cargo receptors in protein traffic, only a few have been identified; their cargo spectrum is unknown and the signals they recognize remain poorly understood. We present here an approach we term "PAIRS" (pairing analysis of cargo receptors), which combines systematic genetic manipulations of yeast with automated microscopy screening, to map the spectrum of cargo for a known receptor or to uncover a novel receptor for a particular cargo. Using PAIRS we followed the fate of ∼150 cargos on the background of mutations in nine putative cargo receptors and identified novel cargo for most of these receptors. Deletion of the Erv14 cargo receptor affected the widest range of cargo. Erv14 substrates have a wide array of functions and structures; however, they are all membrane-spanning proteins of the late secretory pathway or plasma membrane. Proteins residing in these organelles have longer transmembrane domains (TMDs). Detailed examination of one cargo supported the hypothesis that Erv14 dependency reflects the length rather than the sequence of the TMD. The PAIRS approach allowed us to uncover new cargo for known cargo receptors and to obtain an unbiased look at specificity in cargo selection. Obtaining the spectrum of cargo for a cargo receptor allows a novel perspective on its mode of action. The rules that appear to guide Erv14 substrate recognition suggest that sorting of membrane proteins at multiple points in the secretory pathway could depend on the physical properties of TMDs. Such a mechanism would allow diverse proteins to utilize a few receptors without the constraints of evolving location-specific sorting motifs.

46 CFR 154.534 - Cargo pumps and cargo compressors.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pumps and cargo compressors. 154.534 Section 154... SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Cargo and Process Piping Systems § 154.534 Cargo pumps and cargo compressors. Cargo pumps and...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Geelhood, Bruce D.; Wogman, Ned A.

In view of the terrorist threats to the United States, the country needs to consider new vectors and weapons related to nuclear and radiological threats against our homeland. The traditional threat vectors, missiles and bombers, have expanded to include threats arriving through the flow of commerce. The new commerce-related vectors include: sea cargo, truck cargo, rail cargo, and passenger transport. The types of weapons have also expanded beyond nuclear warheads to include radiation dispersal devices (RDD) or ''dirty'' bombs. The consequences of these nuclear and radiological threats are considered. The defense against undesirable materials entering our borders is considered. Themore » radiation and other signatures or potential nuclear and radiological threats are examined along with potential sensors to discover undesirable items in the flow of commerce. Techniques to improve detection are considered. A strategy of primary and secondary screening is proposed to rapidly clear most cargo and carefully examine suspect cargo.« less

49 CFR 1549.109 - Security Directives and Information Circulars.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 9 2010-10-01 2010-10-01 false Security Directives and Information Circulars... SCREENING PROGRAM Operations § 1549.109 Security Directives and Information Circulars. (a) TSA may issue an Information Circular to notify certified cargo screening facilities of security concerns. (b) When TSA...

Flavivirus internalization is regulated by a size-dependent endocytic pathway.

PubMed

Hackett, Brent A; Cherry, Sara

2018-04-17

Flaviviruses enter host cells through the process of clathrin-mediated endocytosis, and the spectrum of host factors required for this process are incompletely understood. Here we found that lymphocyte antigen 6 locus E (LY6E) promotes the internalization of multiple flaviviruses, including West Nile virus, Zika virus, and dengue virus. Perhaps surprisingly, LY6E is dispensable for the internalization of the endogenous cargo transferrin, which is also dependent on clathrin-mediated endocytosis for uptake. Since viruses are substantially larger than transferrin, we reasoned that LY6E may be required for uptake of larger cargoes and tested this using transferrin-coated beads of similar size as flaviviruses. LY6E was indeed required for the internalization of transferrin-coated beads, suggesting that LY6E is selectively required for large cargo. Cell biological studies found that LY6E forms tubules upon viral infection and bead internalization, and we found that tubule formation was dependent on RNASEK, which is also required for flavivirus internalization, but not transferrin uptake. Indeed, we found that RNASEK is also required for the internalization of transferrin-coated beads, suggesting it functions upstream of LY6E. These LY6E tubules resembled microtubules, and we found that microtubule assembly was required for their formation and flavivirus uptake. Since microtubule end-binding proteins link microtubules to downstream activities, we screened the three end-binding proteins and found that EB3 promotes virus uptake and LY6E tubularization. Taken together, these results highlight a specialized pathway required for the uptake of large clathrin-dependent endocytosis cargoes, including flaviviruses. Copyright © 2018 the Author(s). Published by PNAS.

Port Security Strategy 2012

DTIC Science & Technology

2007-06-15

the base -case, a series analysis can be performed by varying the various inputs to the network to examine the impact of potential changes to improve...successfully interrogated was the primary MOE. • Based solely on the cost benefit analysis , the RSTG found that the addition of an Unmanned Surface...cargo. The CBP uses a risk based analysis and intelligence to pre-screen, assess and examine 100% of suspicious containers. The remaining cargo is

Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer

PubMed Central

Michael Frei, Dominik; Hodneland, Erlend; Rios-Mondragon, Ivan; Burtey, Anne; Neumann, Beate; Bulkescher, Jutta; Schölermann, Julia; Pepperkok, Rainer; Gerdes, Hans-Hermann; Kögel, Tanja

2015-01-01

Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT. PMID:26271723

49 CFR 1549.103 - Qualifications and training of individuals with security-related duties.

Code of Federal Regulations, 2011 CFR

2011-10-01

... screening technologies that the facility is authorized to use. These include: (i) The ability to operate x-ray equipment and to distinguish on the x-ray monitor the appropriate imaging standard specified in the certified cargo screening facility security program. Wherever the x-ray system displays colors...

75 FR 63191 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Certified...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-14

...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), OMB control number 1652-0053, abstracted below that we will submit to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. The collections include: (1) Applications from entities that wish to become Certified Cargo Screening Facilities (CCSF) or operate as a TSA- approved validation firm; (2) personal information to allow TSA to conduct security threat assessments on key individuals employed by the CCSFs and validation firms; (3) implementation of a standard security program or submission of a proposed modified security program; (4) information on the amount of cargo screened; (5) recordkeeping requirements for CCSFs and validation firms; and (6) submission of validation reports to TSA. TSA is seeking the renewal of the ICR for the continuation of the program in order to secure passenger aircraft carrying cargo by the deadlines set out in the Implementing Recommendations of the 9/11 Commission Act of 2007.

Fast-neutron/gamma-ray radiography scanner for the detection of contraband in air cargo containers

NASA Astrophysics Data System (ADS)

Eberhardt, J.; Liu, Y.; Rainey, S.; Roach, G.; Sowerby, B.; Stevens, R.; Tickner, J.

2006-05-01

There is a worldwide need for efficient inspection of cargo containers at airports, seaports and road border crossings. The main objectives are the detection of contraband such as illicit drugs, explosives and weapons. Due to the large volume of cargo passing through Australia's airports every day, it is critical that any scanning system should be capable of working on unpacked or consolidated cargo, taking at most 1-2 minutes per container. CSIRO has developed a fast-neutron/gamma-ray radiography (FNGR) method for the rapid screening of air freight. By combining radiographs obtained using 14 MeV neutrons and 60Co gamma-rays, high resolution images showing both density and material composition are obtained. A near full-scale prototype scanner has been successfully tested in the laboratory. With the support of the Australian Customs Service, a full-scale scanner has recently been installed and commissioned at Brisbane International Airport.

The Effect of the Three-Dimensional Geometry of Cargo on the Detection of Radioactive Sources in Cargo Containers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schweppe, John E.; Ely, James H.; McConn, Ronald J.

Pacific Northwest National Laboratory has developed computer models to simulate the screening of vehicles and cargo with radiation portal monitors for the presence of illegitimate radioactive material. In addition, selected measurements have been conducted to validate the models. An important consideration in the modeling of realistic scenarios is the influence of the three-dimensional geometry of the cargo on the measured signature. This is particularly important for scenarios where the source and detector move with respect to each other. Two cases of the influence of the three-dimensional geometry of the cargo on the measured radiation signature are analyzed. In the first,more » measurements show that spectral data collected from moving sources so as to maximize the gross-counting signal-to-noise ratio has minimal spectral distortion, so that the spectral data can be summed over this time interval. In the second, modeling demonstrates that the ability to detect radioactive sources at all locations in a container full of cargo scales approximately linearly with the vertical height of the detector, suggesting that detectors should be approximately the same height as the container they scan.« less

The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles

PubMed Central

Topalidou, Irini; Cattin-Ortolá, Jérôme; MacCoss, Michael J.

2016-01-01

The dense-core vesicle is a secretory organelle that mediates the regulated release of peptide hormones, growth factors, and biogenic amines. Dense-core vesicles originate from the trans-Golgi of neurons and neuroendocrine cells, but it is unclear how this specialized organelle is formed and acquires its specific cargos. To identify proteins that act in dense-core vesicle biogenesis, we performed a forward genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We previously reported the identification of two conserved proteins that interact with the small GTPase RAB-2 to control normal dense-core vesicle cargo-sorting. Here we identify several additional conserved factors important for dense-core vesicle cargo sorting: the WD40 domain protein EIPR-1 and the endosome-associated recycling protein (EARP) complex. By assaying behavior and the trafficking of dense-core vesicle cargos, we show that mutants that lack EIPR-1 or EARP have defects in dense-core vesicle cargo-sorting similar to those of mutants in the RAB-2 pathway. Genetic epistasis data indicate that RAB-2, EIPR-1 and EARP function in a common pathway. In addition, using a proteomic approach in rat insulinoma cells, we show that EIPR-1 physically interacts with the EARP complex. Our data suggest that EIPR-1 is a new interactor of the EARP complex and that dense-core vesicle cargo sorting depends on the EARP-dependent trafficking of cargo through an endosomal sorting compartment. PMID:27191843

Characterizing X-ray Attenuation of Containerized Cargo

DOE Office of Scientific and Technical Information (OSTI.GOV)

Birrer, N.; Divin, C.; Glenn, S.

X-ray inspection systems can be used to detect radiological and nuclear threats in imported cargo. In order to better understand performance of these systems, the attenuation characteristics of imported cargo need to be determined. This project focused on developing image processing algorithms for segmenting cargo and using x-ray attenuation to quantify equivalent steel thickness to determine cargo density. These algorithms were applied to over 450 cargo radiographs. The results are summarized in this report.

Kinesin regulation dynamics through cargo delivery, a single molecule investigation

NASA Astrophysics Data System (ADS)

Kovacs, Anthony; Kessler, Jonathan; Lin, Huawen; Dutcher, Susan; Wang, Yan Mei

2015-03-01

Kinesins are microtubule-based motors that deliver cargo to their destinations in a highly regulated manner. Although in recent years numerous regulators of cargo delivery have been identified, the regulation mechanism of kinesin through the cargo delivery and recycling process is not known. By performing single molecule fluorescence imaging measurements in Chlamydomonas flagella, which are 200 nm in diameter, 10 microns in length, and contain 9 sets of microtubule doublets, we tracked the intraflagellar transport (IFT) trains, BBSome cargo, and kinesin-2 motors through the cargo delivery process and determined the aforementioned dynamics. Upon arrival at the microtubule plus end at the flagellar tip, (1) IFT trains and BBSome cargo remain intact, dissociate together from kinesins and microtubules, and diffuse along flagellar membrane for a mean of 2.3 sec before commencing retrograde travel. (2) Kinesin motors remain bound to and diffuse along microtubules for 1.3 sec before dissociating into the flagellar lumen for recycling.

46 CFR 153.907 - Cargo information.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Cargo information. 153.907 Section 153.907 Shipping... Information § 153.907 Cargo information. (a) The master shall ensure that the following information for each... process for the vessel. (b) The master shall make sure that the following information for cargoes other...

46 CFR 153.907 - Cargo information.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo information. 153.907 Section 153.907 Shipping... Information § 153.907 Cargo information. (a) The master shall ensure that the following information for each... process for the vessel. (b) The master shall make sure that the following information for cargoes other...

46 CFR 154.517 - Piping: Liquid pressure relief.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Piping: Liquid pressure relief. 154.517 Section 154.517... and Process Piping Systems § 154.517 Piping: Liquid pressure relief. The cargo loading and discharge crossover headers, cargo hoses, and cargo loading arms must have means to relieve cargo pressure and to...

46 CFR 154.517 - Piping: Liquid pressure relief.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Piping: Liquid pressure relief. 154.517 Section 154.517... and Process Piping Systems § 154.517 Piping: Liquid pressure relief. The cargo loading and discharge crossover headers, cargo hoses, and cargo loading arms must have means to relieve cargo pressure and to...

46 CFR 154.517 - Piping: Liquid pressure relief.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Piping: Liquid pressure relief. 154.517 Section 154.517... and Process Piping Systems § 154.517 Piping: Liquid pressure relief. The cargo loading and discharge crossover headers, cargo hoses, and cargo loading arms must have means to relieve cargo pressure and to...

46 CFR 154.517 - Piping: Liquid pressure relief.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Piping: Liquid pressure relief. 154.517 Section 154.517... and Process Piping Systems § 154.517 Piping: Liquid pressure relief. The cargo loading and discharge crossover headers, cargo hoses, and cargo loading arms must have means to relieve cargo pressure and to...

46 CFR 154.517 - Piping: Liquid pressure relief.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Piping: Liquid pressure relief. 154.517 Section 154.517... and Process Piping Systems § 154.517 Piping: Liquid pressure relief. The cargo loading and discharge crossover headers, cargo hoses, and cargo loading arms must have means to relieve cargo pressure and to...

Air Cargo Marketing Development

NASA Technical Reports Server (NTRS)

Kersey, J. W.

1972-01-01

The factors involved in developing a market for air cargo services are discussed. A comparison is made between the passenger traffic problems and those of cargo traffic. Emphasis is placed on distribution analyses which isolates total distribution cost, including logistical costs such as transportation, inventory, materials handling, packaging, and processing. Specific examples of methods for reducing air cargo costs are presented.

49 CFR 1549.3 - TSA inspection authority.

Code of Federal Regulations, 2012 CFR

2012-10-01

...) Oversee the implementation, and ensure the adequacy, of security measures at airports and other... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY CERTIFIED CARGO SCREENING PROGRAM General § 1549.3...

49 CFR 1549.3 - TSA inspection authority.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) Oversee the implementation, and ensure the adequacy, of security measures at airports and other... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY CERTIFIED CARGO SCREENING PROGRAM General § 1549.3...

49 CFR 1549.3 - TSA inspection authority.

Code of Federal Regulations, 2013 CFR

2013-10-01

...) Oversee the implementation, and ensure the adequacy, of security measures at airports and other... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY CERTIFIED CARGO SCREENING PROGRAM General § 1549.3...

49 CFR 1549.3 - TSA inspection authority.

Code of Federal Regulations, 2014 CFR

2014-10-01

...) Oversee the implementation, and ensure the adequacy, of security measures at airports and other... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY CERTIFIED CARGO SCREENING PROGRAM General § 1549.3...

Dust prevention in bulk material transportation and handling

NASA Astrophysics Data System (ADS)

Kirichenko, A. V.; Kuznetsov, A. L.; Pogodin, V. A.

2017-10-01

The environmental problem of territory and atmosphere pollution caused by transportation and handling of dust-generating bulk cargo materials is quite common for the whole world. The reducing of weight of fine class coal caused by air blowing reaches the level of 0.5-0.6 t per railcar over the 500 km transportation distance, which is equal to the loss of 1 % of the total weight. The studies showed that all over the country in the process of the railroad transportation, the industry loses 3-5 metric tonnes of coal annually. There are several common tactical measurers to prevent dust formation: treating the dust-producing materials at dispatch point with special liquid solutions; watering the stacks and open handling points of materials; frequent dust removing and working area cleaning. Recently there appeared several new radical measures for pollution prevention in export of ore and coal materials via sea port terminals, specifically: wind-dust protection screens, the container cargo handling system of delivery materials to the hold of the vessels. The article focuses on the discussion of these measures.

Analytical solution of the problem of acceleration of cargo by a bridge crane with constant acceleration at elimination of swings of a cargo rope

NASA Astrophysics Data System (ADS)

Korytov, M. S.; Shcherbakov, V. S.; Titenko, V. V.

2018-01-01

Limitation of the swing of the bridge crane cargo rope is a matter of urgency, as it can significantly improve the efficiency and safety of the work performed. In order to completely dampen the pendulum swing after the break-up of a bridge or a bridge-crane freight cart to maximum speed, it is necessary, in the normal repulsion control of the electric motor, to split the process of dispersion into a minimum of three gaps. For a dynamic system of swinging of a bridge crane on a flexible cable hanger in a separate vertical plane, an analytical solution was obtained to determine the temporal dependence of the cargo rope angle relative to the gravitational vertical when the cargo suspension point moves with constant acceleration. The resulting analytical dependence of the cargo rope angle and its first derivative can break the process of dispersing the cargo suspension point into three stages of dispersal and braking with various accelerations and enter maximum speed of movement of the cargo suspension point. In doing so, the condition of eliminating the swings of the cargo rope relative to the gravitational vertical is fulfilled. Provides examples of the maximum speed output constraints-to-time when removing the rope swing.

TBC-8, a putative RAB-2 GAP, regulates dense core vesicle maturation in Caenorhabditis elegans.

PubMed

Hannemann, Mandy; Sasidharan, Nikhil; Hegermann, Jan; Kutscher, Lena M; Koenig, Sabine; Eimer, Stefan

2012-01-01

Dense core vesicles (DCVs) are thought to be generated at the late Golgi apparatus as immature DCVs, which subsequently undergo a maturation process through clathrin-mediated membrane remodeling events. This maturation process is required for efficient processing of neuropeptides within DCVs and for removal of factors that would otherwise interfere with DCV release. Previously, we have shown that the GTPase, RAB-2, and its effector, RIC-19, are involved in DCV maturation in Caenorhabditis elegans motoneurons. In rab-2 mutants, specific cargo is lost from maturing DCVs and missorted into the endosomal/lysosomal degradation route. Cargo loss could be prevented by blocking endosomal delivery. This suggests that RAB-2 is involved in retention of DCV components during the sorting process at the Golgi-endosomal interface. To understand how RAB-2 activity is regulated at the Golgi, we screened for RAB-2-specific GTPase activating proteins (GAPs). We identified a potential RAB-2 GAP, TBC-8, which is exclusively expressed in neurons and which, when depleted, shows similar DCV maturation defects as rab-2 mutants. We could demonstrate that RAB-2 binds to its putative GAP, TBC-8. Interestingly, TBC-8 also binds to the RAB-2 effector, RIC-19. This interaction appears to be conserved as TBC-8 also interacted with the human ortholog of RIC-19, ICA69. Therefore, we propose that a dynamic ON/OFF cycling of RAB-2 at the Golgi induced by the GAP/effector complex is required for proper DCV maturation.

Cargo/Logistics Airlift System Study (CLASS), Volume 1

NASA Technical Reports Server (NTRS)

Norman, J. M.; Henderson, R. D.; Macey, F. C.; Tuttle, R. P.

1978-01-01

Current and advanced air cargo systems are evaluated using industrial and consumer statistics. Market and commodity characteristics that influence the use of the air mode are discussed along with a comparison of air and surface mode on typical routes. Results of on-site surveys of cargo processing facilities at airports are presented, and institutional controls and influences on air cargo operations are considered.

49 CFR 1522.127 - Assessment report.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program...) The assessment report must include the following information, in addition to any other information...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Voytchev, M; Radev, R; Chiaro, P

The International Electrotechnical Commission (IEC) is the leading and oldest global organization with over 100 years history of developing and publishing international standards for all electrical, electronic and related technologies, including radiation detection instrumentation. Subcommittee 45B 'Radiation Protection Instrumentation' of the IEC has recently started the development of two standards on radiation-generating devices. IEC 62463 'Radiation protection instrumentation--X-ray Systems for the Screening of Persons for Security and the Carrying of Illicit Items' is applicable to X-ray systems designed for screening people to detect if they are carrying objects such as weapons, explosives, chemical and biological agents and other concealed itemsmore » that could be used for criminal purposes, e.g. terrorist use, drug smuggling, etc. IEC 62523 'Radiation protection instrumentation--Cargo/Vehicle radiographic inspection systems' applies to cargo/vehicle imaging inspection systems using accelerator produced X-ray or gamma radiation to obtain images of the screened objects (e.g. cargo containers, transport and passenger vehicles and railroad cars). The objective of both standards is to specify standard requirements and general characteristics and test procedures, as well as, radiation, electrical, environmental, mechanical, and safety requirements and to provide examples of acceptable methods to test these requirements. In particular the standards address the design requirements as they relate to the radiation protection of the people being screened, people who are in the vicinity of the equipment and the operators. The standard IEC 62463 does not deal with the performance requirements for the quality of the object detection. Compliance with the standards requirements will provide the manufacturers with internationally acceptable specifications and the device users with assurance of the rigorous quality and accuracy of the measurements in relation to the radiological safety of the equipment. The main characteristics of IEC 62463 and IEC 62523 standards are presented and as well as the IEC methodology of standard development and approval.« less

Extensive cargo identification reveals distinct biological roles of the 12 importin pathways.

PubMed

Kimura, Makoto; Morinaka, Yuriko; Imai, Kenichiro; Kose, Shingo; Horton, Paul; Imamoto, Naoko

2017-01-24

Vast numbers of proteins are transported into and out of the nuclei by approximately 20 species of importin-β family nucleocytoplasmic transport receptors. However, the significance of the multiple parallel transport pathways that the receptors constitute is poorly understood because only limited numbers of cargo proteins have been reported. Here, we identified cargo proteins specific to the 12 species of human import receptors with a high-throughput method that employs stable isotope labeling with amino acids in cell culture, an in vitro reconstituted transport system, and quantitative mass spectrometry. The identified cargoes illuminated the manner of cargo allocation to the receptors. The redundancies of the receptors vary widely depending on the cargo protein. Cargoes of the same receptor are functionally related to one another, and the predominant protein groups in the cargo cohorts differ among the receptors. Thus, the receptors are linked to distinct biological processes by the nature of their cargoes.

Microencapsulation and characterization of liposomal vesicles using a supercritical fluid process coupled with vacuum-driven cargo loading.

PubMed

Tsai, Wen-Chyan; Rizvi, Syed S H

2017-06-01

A new technique of liposomal microencapsulation, consisting of supercritical fluid extraction followed by rapid expansion of the supercritical solution and vacuum-driven cargo loading, was successfully developed. It is a continuous flow-through process without usage of any toxic organic solvent. For use as a coating material, the solubility of soy phospholipids in supercritical carbon dioxide was first determined using a dynamic equilibrium system and the data was correlated with the Chrastil model with good agreement. Liposomes were made with D-(+)-glucose as a cargo and their properties were characterized as functions of expansion pressure, temperature, and cargo loading rates. The highest encapsulation efficiency attained was 31.7% at the middle expansion pressure of 12.41MPa, highest expansion temperature of 90°C, and lowest cargo loading rate of 0.25mL/s. The large unilamellar vesicles and multivesicular vesicles were observed to be a majority of the liposomes produced using this eco-friendly process. Copyright © 2017 Elsevier Ltd. All rights reserved.

Cargo container inspection test program at ARPA's Nonintrusive Inspection Technology Testbed

NASA Astrophysics Data System (ADS)

Volberding, Roy W.; Khan, Siraj M.

1994-10-01

An x-ray-based cargo inspection system test program is being conducted at the Advanced Research Project Agency (ARPA)-sponsored Nonintrusive Inspection Technology Testbed (NITT) located in the Port of Tacoma, Washington. The test program seeks to determine the performance that can be expected from a dual, high-energy x-ray cargo inspection system when inspecting ISO cargo containers. This paper describes an intensive, three-month, system test involving two independent test groups, one representing the criminal smuggling element and the other representing the law enforcement community. The first group, the `Red Team', prepares ISO containers for inspection at an off-site facility. An algorithm randomly selects and indicates the positions and preparation of cargoes within a container. The prepared container is dispatched to the NITT for inspection by the `Blue Team'. After in-gate processing, it is queued for examination. The Blue Team inspects the container and decides whether or not to pass the container. The shipment undergoes out-gate processing and returns to the Red Team. The results of the inspection are recorded for subsequent analysis. The test process, including its governing protocol, the cargoes, container preparation, the examination and results available at the time of submission are presented.

49 CFR 1522.129 - Recordkeeping requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program § 1522.129 Recordkeeping requirements. (a) Each validation firm must maintain records... operation as a validation firm, including the records listed below. (b) Each validation firm must retain the...

49 CFR 1522.101 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program § 1522.101 Applicability. This subpart governs the use of TSA-approved validation firms and validators to...

49 CFR 1522.119 - Training.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program § 1522.119 Training. (a) Initial training. The validation firm must ensure that its validators and...

75 FR 9919 - Extension of Agency Information Collection Activity Under OMB Review: Air Cargo Security...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-04

...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), OMB control number 1652-0040, abstracted below to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on November 16, 2009, 74 FR 58969. TSA has not received any comments. The collections of information that make up this ICR involve five broad categories affecting airports, passenger aircraft operators, foreign air carriers, indirect air carriers operating under a security program, and all-cargo carriers. These five categories are: security programs, security threat assessments (STA), known shipper data via the Known Shipper Management System (KSMS), cargo screening reporting, and evidence of compliance recordkeeping.

76 FR 4362 - Extension of Agency Information Collection Activity Under OMB Review: Air Cargo Security...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-25

...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), OMB control number 1652-0040, abstracted below, to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of this collection of information on October 14, 2010, 75 FR 63192. TSA has not received any comments. The collections of information that make up this ICR involve five broad categories affecting airports, passenger aircraft operators, foreign air carriers, indirect air carriers operating under a security program, and all-cargo carriers. These five categories are: Security programs, security threat assessments (STA), known shipper data via the Known Shipper Management System (KSMS), cargo screening reporting, and evidence of compliance recordkeeping.

High-Throughput Identification of Combinatorial Ligands for DNA Delivery in Cell Culture

NASA Astrophysics Data System (ADS)

Svahn, Mathias G.; Rabe, Kersten S.; Barger, Geoffrey; EL-Andaloussi, Samir; Simonson, Oscar E.; Didier, Boturyn; Olivier, Renaudet; Dumy, Pascal; Brandén, Lars J.; Niemeyer, Christof M.; Smith, C. I. Edvard

2008-10-01

Finding the optimal combinations of ligands for tissue-specific delivery is tedious even if only a few well-established compounds are tested. The cargo affects the receptor-ligand interaction, especially when it is charged like DNA. The ligand should therefore be evaluated together with its cargo. Several viruses have been shown to interact with more than one receptor, for efficient internalization. We here present a DNA oligonucleotide-based method for inexpensive and rapid screening of biotin labeled ligands for combinatorial effects on cellular binding and uptake. The oligonucleotide complex was designed as a 44 bp double-stranded DNA oligonucleotide with one central streptavidin molecule and a second streptavidin at the terminus. The use of a highly advanced robotic platform ensured stringent processing and execution of the experiments. The oligonucleotides were fluorescently labeled and used for detection and analysis of cell-bound, internalized and intra-cellular compartmentalized constructs by an automated line-scanning confocal microscope, IN Cell Analyzer 3000. All possible combinations of 22 ligands were explored in sets of 2 and tested on 6 different human cell lines in triplicates. In total, 10 000 transfections were performed on the automation platform. Cell-specific combinations of ligands were identified and their relative position on the scaffold oligonucleotide was found to be of importance. The ligands were found to be cargo dependent, carbohydrates were more potent for DNA delivery whereas cell penetrating peptides were more potent for delivery of less charged particles.

Aviation security cargo inspection queuing simulation model for material flow and accountability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olama, Mohammed M; Allgood, Glenn O; Rose, Terri A

Beginning in 2010, the U.S. will require that all cargo loaded in passenger aircraft be inspected. This will require more efficient processing of cargo and will have a significant impact on the inspection protocols and business practices of government agencies and the airlines. In this paper, we develop an aviation security cargo inspection queuing simulation model for material flow and accountability that will allow cargo managers to conduct impact studies of current and proposed business practices as they relate to inspection procedures, material flow, and accountability.

49 CFR 1522.125 - Protection of information.

Code of Federal Regulations, 2010 CFR

2010-10-01

... VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program § 1522.125 Protection of information. (a) Sensitive Security Information. Each validation... 49 Transportation 9 2010-10-01 2010-10-01 false Protection of information. 1522.125 Section 1522...

49 CFR 1522.113 - Withdrawal of approval.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program... TSA-approved validation firm if the validation firm ceases to meet the standards for approval, fails...

76 FR 60755 - Air Cargo Screening; Reopening of Comment Period

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-30

... activities, the disclosure of which would constitute an unwarranted invasion of privacy, reveal trade secrets... secrets, confidential commercial or financial information, or SSI to the public regulatory docket. Please.... TSA will hold documents containing SSI, confidential business information, or trade secrets in a...

49 CFR 1522.123 - Conduct of assessments.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program... immediately to TSA. (d) No authorization to take remedial or disciplinary action. Neither the validation firm...

A Hopping Mechanism for Cargo Transport by Molecular Motors on Crowded Microtubules

NASA Astrophysics Data System (ADS)

Goldman, Carla

2010-05-01

Most models designed to study the bidirectional movement of cargos as they are driven by molecular motors rely on the idea that motors of different polarities can be coordinated by external agents if arranged into a motor-cargo complex to perform the necessary work Gross, Hither and yon: a review of bidirectional microtubule-based transport (Gross in Phys. Biol. 1:R1-R11, 2004). Although these models have provided us with important insights into these phenomena, there are still many unanswered questions regarding the mechanisms through which the movement of the complex takes place on crowded microtubules. For example (i) how does cargo-binding affect motor motility? and in connection with that - (ii) how does the presence of other motors (and also other cargos) on the microtubule affect the motility of the motor-cargo complex? We discuss these questions from a different perspective. The movement of a cargo is conceived here as a hopping process resulting from the transference of cargo between neighboring motors. In the light of this, we examine the conditions under which cargo might display bidirectional movement even if directed by motors of a single polarity. The global properties of the model in the long-time regime are obtained by mapping the dynamics of the collection of interacting motors and cargos into an asymmetric simple exclusion process (ASEP) which can be resolved using the matrix ansatz introduced by Derrida (Derrida and Evans in Nonequilibrium Statistical Mechanics in One Dimension, pp. 277-304, 1997; Derrida et al. in J. Phys. A 26:1493-1517, 1993).

Scanning of vehicles for nuclear materials

NASA Astrophysics Data System (ADS)

Katz, J. I.

2014-05-01

Might a nuclear-armed terrorist group or state use ordinary commerce to deliver a nuclear weapon by smuggling it in a cargo container or vehicle? This delivery method would be the only one available to a sub-state actor, and it might enable a state to make an unattributed attack. Detection of a weapon or fissile material smuggled in this manner is difficult because of the large volume and mass available for shielding. Here I review methods for screening cargo containers to detect the possible presence of nuclear threats. Because of the large volume of innocent international commerce, and the cost and disruption of secondary screening by opening and inspection, it is essential that the method be rapid and have a low false-positive rate. Shielding can prevent the detection of neutrons emitted spontaneously or by induced fission. The two promising methods are muon tomography and high energy X-radiography. If they do not detect a shielded threat object they can detect the shield itself.

Scanning of vehicles for nuclear materials

DOE Office of Scientific and Technical Information (OSTI.GOV)

Katz, J. I.

2014-05-09

Might a nuclear-armed terrorist group or state use ordinary commerce to deliver a nuclear weapon by smuggling it in a cargo container or vehicle? This delivery method would be the only one available to a sub-state actor, and it might enable a state to make an unattributed attack. Detection of a weapon or fissile material smuggled in this manner is difficult because of the large volume and mass available for shielding. Here I review methods for screening cargo containers to detect the possible presence of nuclear threats. Because of the large volume of innocent international commerce, and the cost andmore » disruption of secondary screening by opening and inspection, it is essential that the method be rapid and have a low false-positive rate. Shielding can prevent the detection of neutrons emitted spontaneously or by induced fission. The two promising methods are muon tomography and high energy X-radiography. If they do not detect a shielded threat object they can detect the shield itself.« less

46 CFR 154.530 - Valves: Cargo tank MARVS 69 kPa gauge (10 psig) or lower.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Valves: Cargo tank MARVS 69 kPa gauge (10 psig) or lower..., Construction and Equipment Cargo and Process Piping Systems § 154.530 Valves: Cargo tank MARVS 69 kPa gauge (10... a MARVS of 69 kPa gauge (10 psig) or lower must have shut-off valves that— (1) Are located as close...

Combining reflectometry and fluorescence microscopy: an assay for the investigation of leakage processes across lipid membranes.

PubMed

Stephan, Milena; Mey, Ingo; Steinem, Claudia; Janshoff, Andreas

2014-02-04

The passage of solutes across a lipid membrane plays a central role in many cellular processes. However, the investigation of transport processes remains a serious challenge in pharmaceutical research, particularly the transport of uncharged cargo. While translocation reactions of ions across cell membranes is commonly measured with the patch-clamp, an equally powerful screening method for the transport of uncharged compounds is still lacking. A combined setup for reflectometric interference spectroscopy (RIfS) and fluorescence microscopy measurements is presented that allows one to investigate the passive exchange of uncharged compounds across a free-standing membrane. Pore-spanning lipid membranes were prepared by spreading giant 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles on porous anodic aluminum oxide (AAO) membranes, creating sealed attoliter-sized compartments. The time-resolved leakage of different dye molecules (pyranine and crystal violet) as well as avidin through melittin induced membrane pores and defects was investigated.

Site Preparation Guide: Increment 1 and Increment 2. Cargo Movement Operations System (CMOS). Revision

DTIC Science & Technology

1990-03-01

Freight, and Air Freight workcen- ters. Increment II workcenters will also use these computers. All order processing , cargo information processing...4. Work Clearance Permits .................................... 47 5. Work Order Processing ..................................... 47 6. Validation...implementation. 5. Work Order Processing . a. After SSC/AQFT/AQAE have reviewed and approved the site PSA, the site will be notified to begin

49 CFR Appendix A to Part 1511 - Aviation Security Infrastructure Fee

Code of Federal Regulations, 2010 CFR

2010-10-01

... costs of checkpoint screening personnel. 2. Salary, benefits, overtime, retirement and other costs of exit lane monitors. 3. Salary, benefits, overtime, retirement and other costs of cargo screeners. 4. Salary, benefits, overtime, retirement and other costs of checked baggage screeners. 5. Salary, benefits...

49 CFR 1544.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2013 CFR

2013-10-01

... provided in its security program. Such methods may include TSA-approved x-ray systems, explosives detection systems, explosives trace detection, explosives detection canine teams certified by TSA, or a physical...) Preventing or deterring the carriage of any explosive or incendiary. Each aircraft operator operating under a...

49 CFR 1544.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2011 CFR

2011-10-01

... provided in its security program. Such methods may include TSA-approved x-ray systems, explosives detection systems, explosives trace detection, explosives detection canine teams certified by TSA, or a physical...) Preventing or deterring the carriage of any explosive or incendiary. Each aircraft operator operating under a...

49 CFR 1544.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2012 CFR

2012-10-01

... provided in its security program. Such methods may include TSA-approved x-ray systems, explosives detection systems, explosives trace detection, explosives detection canine teams certified by TSA, or a physical...) Preventing or deterring the carriage of any explosive or incendiary. Each aircraft operator operating under a...

49 CFR 1544.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2014 CFR

2014-10-01

... provided in its security program. Such methods may include TSA-approved x-ray systems, explosives detection systems, explosives trace detection, explosives detection canine teams certified by TSA, or a physical...) Preventing or deterring the carriage of any explosive or incendiary. Each aircraft operator operating under a...

TBC-8, a Putative RAB-2 GAP, Regulates Dense Core Vesicle Maturation in Caenorhabditis elegans

PubMed Central

Hannemann, Mandy; Sasidharan, Nikhil; Hegermann, Jan; Kutscher, Lena M.; Koenig, Sabine; Eimer, Stefan

2012-01-01

Dense core vesicles (DCVs) are thought to be generated at the late Golgi apparatus as immature DCVs, which subsequently undergo a maturation process through clathrin-mediated membrane remodeling events. This maturation process is required for efficient processing of neuropeptides within DCVs and for removal of factors that would otherwise interfere with DCV release. Previously, we have shown that the GTPase, RAB-2, and its effector, RIC-19, are involved in DCV maturation in Caenorhabditis elegans motoneurons. In rab-2 mutants, specific cargo is lost from maturing DCVs and missorted into the endosomal/lysosomal degradation route. Cargo loss could be prevented by blocking endosomal delivery. This suggests that RAB-2 is involved in retention of DCV components during the sorting process at the Golgi-endosomal interface. To understand how RAB-2 activity is regulated at the Golgi, we screened for RAB-2–specific GTPase activating proteins (GAPs). We identified a potential RAB-2 GAP, TBC-8, which is exclusively expressed in neurons and which, when depleted, shows similar DCV maturation defects as rab-2 mutants. We could demonstrate that RAB-2 binds to its putative GAP, TBC-8. Interestingly, TBC-8 also binds to the RAB-2 effector, RIC-19. This interaction appears to be conserved as TBC-8 also interacted with the human ortholog of RIC-19, ICA69. Therefore, we propose that a dynamic ON/OFF cycling of RAB-2 at the Golgi induced by the GAP/effector complex is required for proper DCV maturation. PMID:22654674

46 CFR 154.519 - Piping relief valves.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Process Piping Systems § 154.519 Piping relief valves. (a) The liquid relief valve that protects the cargo piping system from liquid pressure exceeding the design pressure must discharge into: (1) A cargo tank; or (2) A cargo vent mast if that vent mast has a means for the detection and removal of the liquid...

46 CFR 154.519 - Piping relief valves.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Process Piping Systems § 154.519 Piping relief valves. (a) The liquid relief valve that protects the cargo piping system from liquid pressure exceeding the design pressure must discharge into: (1) A cargo tank; or (2) A cargo vent mast if that vent mast has a means for the detection and removal of the liquid...

46 CFR 154.519 - Piping relief valves.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Process Piping Systems § 154.519 Piping relief valves. (a) The liquid relief valve that protects the cargo piping system from liquid pressure exceeding the design pressure must discharge into: (1) A cargo tank; or (2) A cargo vent mast if that vent mast has a means for the detection and removal of the liquid...

46 CFR 154.519 - Piping relief valves.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Process Piping Systems § 154.519 Piping relief valves. (a) The liquid relief valve that protects the cargo piping system from liquid pressure exceeding the design pressure must discharge into: (1) A cargo tank; or (2) A cargo vent mast if that vent mast has a means for the detection and removal of the liquid...

Study on the multi-sensors monitoring and information fusion technology of dangerous cargo container

NASA Astrophysics Data System (ADS)

Xu, Shibo; Zhang, Shuhui; Cao, Wensheng

2017-10-01

In this paper, monitoring system of dangerous cargo container based on multi-sensors is presented. In order to improve monitoring accuracy, multi-sensors will be applied inside of dangerous cargo container. Multi-sensors information fusion solution of monitoring dangerous cargo container is put forward, and information pre-processing, the fusion algorithm of homogenous sensors and information fusion based on BP neural network are illustrated, applying multi-sensors in the field of container monitoring has some novelty.

Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy.

PubMed

Mancias, Joseph D; Wang, Xiaoxu; Gygi, Steven P; Harper, J Wade; Kimmelman, Alec C

2014-05-01

Autophagy, the process by which proteins and organelles are sequestered in double-membrane structures called autophagosomes and delivered to lysosomes for degradation, is critical in diseases such as cancer and neurodegeneration. Much of our understanding of this process has emerged from analysis of bulk cytoplasmic autophagy, but our understanding of how specific cargo, including organelles, proteins or intracellular pathogens, are targeted for selective autophagy is limited. Here we use quantitative proteomics to identify a cohort of novel and known autophagosome-enriched proteins in human cells, including cargo receptors. Like known cargo receptors, nuclear receptor coactivator 4 (NCOA4) was highly enriched in autophagosomes, and associated with ATG8 proteins that recruit cargo-receptor complexes into autophagosomes. Unbiased identification of NCOA4-associated proteins revealed ferritin heavy and light chains, components of an iron-filled cage structure that protects cells from reactive iron species but is degraded via autophagy to release iron through an unknown mechanism. We found that delivery of ferritin to lysosomes required NCOA4, and an inability of NCOA4-deficient cells to degrade ferritin led to decreased bioavailable intracellular iron. This work identifies NCOA4 as a selective cargo receptor for autophagic turnover of ferritin (ferritinophagy), which is critical for iron homeostasis, and provides a resource for further dissection of autophagosomal cargo-receptor connectivity.

77 FR 30542 - Air Cargo Screening Fees

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-23

... Security Identification Display Area (SIDA) IDs. Another commenter requested that if TSA intends to limit... obtain SIDA IDs were not included in the fee models for this rulemaking because STAs for holders of SIDA... FR 51854. Comment: One commenter appreciated TSA's recognition that the STAs performed under the SIDA...

49 CFR 1549.3 - TSA inspection authority.

Code of Federal Regulations, 2010 CFR

2010-10-01

... transportation facilities; (vi) Review security plans; and (vii) Carry out such other duties, and exercise such... access media or identification media issued or approved by a certified cargo screening facility or other person, except that the TSA and DHS officials will have identification media issued by TSA or DHS. ...

49 CFR 1546.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2014 CFR

2014-10-01

... in its security program. Such methods may include TSA-approved x-ray systems, explosives detection systems, explosives trace detection, explosives detection canine teams certified by TSA, a physical search... of any explosive or incendiary. Each foreign air carrier operating a program under § 1546.101(a), (b...

49 CFR 1546.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2012 CFR

2012-10-01

... in its security program. Such methods may include TSA-approved x-ray systems, explosives detection systems, explosives trace detection, explosives detection canine teams certified by TSA, a physical search... of any explosive or incendiary. Each foreign air carrier operating a program under § 1546.101(a), (b...

49 CFR 1546.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2013 CFR

2013-10-01

... in its security program. Such methods may include TSA-approved x-ray systems, explosives detection systems, explosives trace detection, explosives detection canine teams certified by TSA, a physical search... of any explosive or incendiary. Each foreign air carrier operating a program under § 1546.101(a), (b...

49 CFR 1546.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2011 CFR

2011-10-01

... in its security program. Such methods may include TSA-approved x-ray systems, explosives detection systems, explosives trace detection, explosives detection canine teams certified by TSA, a physical search... of any explosive or incendiary. Each foreign air carrier operating a program under § 1546.101(a), (b...

49 CFR 1522.109 - TSA review and approval.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program... that the applicant is qualified to be a validation firm. (b) Notice.—(1) Approval. If an application is...

77 FR 24506 - Extension of Agency Information Collection Activity Under OMB Review: Air Cargo Security...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-24

...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0040, abstracted below to OMB for review and approval of an extension of the currently approved collection under the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on February 24, 2012, 77 FR 11145. TSA has not received any comments. The collection of information that make up this ICR involve five broad categories affecting airports, passenger aircraft operators, foreign air carriers, indirect air carriers and all-cargo carriers operating under a TSA-approved security program. These five categories are: Security programs, security threat assessments (STAs), known shipper data via the Known Shipper Management System (KSMS), cargo screening reporting, and evidence of compliance recordkeeping.

Development of efficiency module of organization of Arctic sea cargo transportation with application of neural network technologies

NASA Astrophysics Data System (ADS)

Sobolevskaya, E. Yu; Glushkov, S. V.; Levchenko, N. G.; Orlov, A. P.

2018-05-01

The analysis of software intended for organizing and managing the processes of sea cargo transportation has been carried out. The shortcomings of information resources are presented, for the organization of work in the Arctic and Subarctic regions of the Far East: the lack of decision support systems, the lack of factor analysis to calculate the time and cost of delivery. The architecture of the module for calculating the effectiveness of the organization of sea cargo transportation has been developed. The simulation process has been considered, which is based on the neural network. The main classification factors with their weighting coefficients have been identified. The architecture of the neural network has been developed to calculate the efficiency of the organization of sea cargo transportation in Arctic conditions. The architecture of the intellectual system of organization of sea cargo transportation has been developed, taking into account the difficult navigation conditions in the Arctic. Its implementation will allow one to provide the management of the shipping company with predictive analytics; to support decision-making; to calculate the most efficient delivery route; to provide on demand online transportation forecast, to minimize the shipping cost, delays in transit, and risks to cargo safety.

Enhanced Images for Checked and Carry-on Baggage and Cargo Screening

NASA Technical Reports Server (NTRS)

Woodell, Glenn; Rahman, Zia-ur; Jobson, Daniel J.; Hines, Glenn

2004-01-01

The current X-ray systems used by airport security personnel for the detection of contraband, and objects such as knives and guns that can impact the security of a flight, have limited effect because of the limited display quality of the X-ray images. Since the displayed images do not possess optimal contrast and sharpness, it is possible for the security personnel to miss potentially hazardous objects. This problem is also common to other disciplines such as medical Xrays, and can be mitigated, to a large extent, by the use of state-of-the-art image processing techniques to enhance the contrast and sharpness of the displayed image. The NASA Langley Research Center's Visual Information Processing Group has developed an image enhancement technology that has direct applications to this problem of inadequate display quality. Airport security X-ray imaging systems would benefit considerably by using this novel technology, making the task of the personnel who have to interpret the X-ray images considerably easier, faster, and more reliable. This improvement would translate into more accurate screening as well as minimizing the screening time delays to airline passengers. This technology, Retinex, has been optimized for consumer applications but has been applied to medical X-rays on a very preliminary basis. The resultant technology could be incorporated into a new breed of commercial x-ray imaging systems which would be transparent to the screener yet allow them to see subtle detail much more easily, reducing the amount of time needed for screening while greatly increasing the effectiveness of contraband detection and thus public safety.

Enhanced Images for Checked and Carry-on Baggage and Cargo Screening

NASA Technical Reports Server (NTRS)

Woodell, Glen; Rahman, Zia-ur; Jobson, Daniel J.; Hines, Glenn

2004-01-01

The current X-ray systems used by airport security personnel for the detection of contraband, and objects such as knives and guns that can impact the security of a flight, have limited effect because of the limited display quality of the X-ray images. Since the displayed images do not possess optimal contrast and sharpness, it is possible for the security personnel to miss potentially hazardous objects. This problem is also common to other disciplines such as medical X-rays, and can be mitigated, to a large extent, by the use of state-of-the-art image processing techniques to enhance the contrast and sharpness of the displayed image. The NASA Langley Research Centers Visual Information Processing Group has developed an image enhancement technology that has direct applications to this problem of inadequate display quality. Airport security X-ray imaging systems would benefit considerably by using this novel technology, making the task of the personnel who have to interpret the X-ray images considerably easier, faster, and more reliable. This improvement would translate into more accurate screening as well as minimizing the screening time delays to airline passengers. This technology, Retinex, has been optimized for consumer applications but has been applied to medical X-rays on a very preliminary basis. The resultant technology could be incorporated into a new breed of commercial x-ray imaging systems which would be transparent to the screener yet allow them to see subtle detail much more easily, reducing the amount of time needed for screening while greatly increasing the effectiveness of contraband detection and thus public safety.

77 FR 12321 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Enhanced...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-29

... information on those who are to respond, including using appropriate automated, electronic, mechanical, or... for DCA, the operator must inspect the aircraft and screen the passengers, their carry-on property, and property carried in the cargo hold. For each passenger and crewmember onboard an aircraft that...

49 CFR 1546.205 - Acceptance and screening of cargo.

Code of Federal Regulations, 2010 CFR

2010-10-01

... system prescribed by this part. (c) Control. Each foreign air carrier operating a program under § 1546... of any explosive or incendiary. Each foreign air carrier operating a program under § 1546.101(a), (b... air carrier operating a program under § 1546.101(a), (b), (e), or (f) must refuse to transport any...

77 FR 12069 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-28

... the information collection and its expected burden. The collection requires interested volunteers to... to establish a program to deputize volunteer pilots of passenger air carriers as Federal law... cargo aircraft. In order to screen volunteers for entry into the FFDO program, TSA collects information...

78 FR 26382 - Advisory Committee on Commercial Operations of Customs and Border Protection (COAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-06

... Completed by the Export Mapping Working Group (EMWG) to date. 3. Review and Discuss the Global Supply Chain Subcommittee's Air Cargo Advance Screening (ACAS) Working Group and address Next Steps regarding Land Border... the Trusted Trader Subcommittee and the Work Completed by the Industry Standards Working Group (ISWG...

76 FR 4709 - Extension of Agency Information Collection Activity Under OMB Review: Certified Cargo Screening...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-26

... period soliciting comments, of the following collection of information on October 14, 2010, 75 FR 63191. TSA has received no comments. The collections include: (1) Applications from entities that wish to... CCSFs and validation firms; (3) implementation of a standard security program or submission of a...

Revenue management of air cargo service in theory and practice

NASA Astrophysics Data System (ADS)

Budiarto, S.; Putro, H. P.; Pradono, P.; Yudoko, G.

2018-05-01

This study examines the air cargo service by comparing existing theories from previous research with the conditions on the ground. The object of the study is focused on the freight forwarder and the airport management. This study reviews the models and results of previous research that will be summarized and used to identify any issues related to the characteristics of air cargo operational services, as well as observing and monitoring literature with airlines, shipping companies, and airport management to explore and see the gap between prior research and implementation of its process in the air cargo service. The first phase in this study is to provide an overview of the air cargo industry. The second phase analyzes the characteristic differences between air cargo services and air passenger operating services. And the third phase is a literary bibliography study of air cargo operations, where the focus is on the studies using quantitative models from the perspective of the object of the study, which is the optimization of revenue management on air cargo services. From the results of the study, which is based on the gap between theory and practice, new research opportunities which are related to management of air cargo service revenue in the form of model development are found by adding booking timelines aspects of cargo that can affect the revenue of cargo airline companies and airports.

CargoTIPS: an innovative approach to combating cargo theft

NASA Astrophysics Data System (ADS)

Toth, Gail E.

1998-12-01

Cargo theft has been estimated by the Federal Bureau o Investigations to be 6 billion annually, while others believe it to be more than 10 billion annually. Opportunistic thieves, street gangs, traditional organized crime groups, and new organized crime groups have been targeting cargo. They steal from warehouses, terminals, equipment, truck stops, or any place where freight comes to a rest. With zero inventory levels, our trailers have become virtual warehouses on wheels and easy targets for thieves. Without information and communication cargo thieves can thrive. The industry and law enforcement are forced into being reactive instead of developing proactive policies and procedures. Cargo thieves cross town lines, county lines, state lines and country borders. This makes communication within the law enforcement community imperative. CargoTIPS (cargo theft information processing system) was developed in response to the need for cargo theft information. The system allows us to collect cargo theft statistics to analyze the problem, assess the threat and develop a response on a national level. CargoTIPS includes a bulletin board, which allows users to communicate with each other, pass on alerts or seek information. The system is also used as an investigative tool. CargoTIPS can identify the mode of transportation (truck, small parcel, air, rail or ocean). It was designed to take in international data. Currently the system has identified that food products are the number one targeted commodity, followed by electronic products and third, computers and computer parts.

An Airbus arrives at KSC with third MPLM

NASA Technical Reports Server (NTRS)

2001-01-01

An Airbus '''Beluga''' air cargo plane, The Super Transporter, lands at KSC's Shuttle Landing Facility. Its cargo, from the factory of Alenia Aerospazio in Turin, Italy, is the Italian Space Agency's Multi-Purpose Logistics Module Donatello, the third of three for the International Space Station. The module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

An Airbus arrives at KSC with third MPLM

NASA Technical Reports Server (NTRS)

2001-01-01

An Airbus '''Beluga''' air cargo plane, The Super Transporter, arrives at KSC's Shuttle Landing Facility from the factory of Alenia Aerospazio in Turin, Italy. Its cargo is the Italian Space Agency's Multi-Purpose Logistics Module Donatello, the third of three for the International Space Station. The module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

KSC-2012-2907

NASA Image and Video Library

2012-05-22

CAPE CANAVERAL, Fla. – Participating in a post-launch news conference in the Press Site auditorium at NASA’s Kennedy Space Center in Florida are, from left, George H. Diller, NASA Public Affairs, William Gerstenmaier, associate administrator of NASA’s Human Exploration and Operations Directorate, Alan Lindenmoyer, manager of NASA’s Commercial Crew and Cargo Program, and Gwynne Shotwell, president of SpaceX. Also participating by video teleconference, on the screen at right, is Elon Musk, chief executive officer and chief designer for SpaceX. The SpaceX Falcon 9 rocket launched into space at 3:44 a.m. EDT from Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. The launch is the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services Program, or COTS. During the flight, the capsule will conduct a series of check-out procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. If the capsule performs as planned, the cargo and experiments it is carrying will be transferred to the station. The cargo includes food, water and provisions for the station’s Expedition crews, such as clothing, batteries and computer equipment. Under COTS, NASA has partnered with two aerospace companies to deliver cargo to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Kim Shiflett

Multipurpose Cargo Transfer Bag

NASA Technical Reports Server (NTRS)

Broyan, James; Baccus, Shelley

2014-01-01

The Logistics Reduction (LR) project within the Advanced Exploration Systems (AES) program is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag, the suitcase-shaped common logistics carrying bag for Shuttle and the International Space Station. After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unzipped, unsnapped, and unfolded to be reused. Reuse ideas that have been investigated include partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing.

A Reversible Light-Operated Nanovalve on Mesoporous Silica Nanoparticles

PubMed Central

Tarn, Derrick; Ferris, Daniel P.; Barnes, Jonathan C.; Ambrogio, Michael W.; Stoddart, J. Fraser

2014-01-01

Two azobenzene α-cyclodextrin based nanovalves are designed, synthesized and assembled on mesoporous silica nanoparticles. When in aqueous conditions, the cyclodextrin cap is tightly bound to the azobenzene moiety and capable of holding back loaded cargo molecules. Upon irradiation with a near-UV light laser, trans to cis- photoisomerization of azobenzene initiates a dethreading process, which causes the cyclodextrin cap to unbind followed by the release of cargo. The addition of a bulky stopper group to the end of the stalk allows this design to be reversible; complete dethreading of cyclodextrin as a result of unbinding with azobenzene is prevented as a consequence of steric interference. As a result, thermal relaxation of cis- to trans-azobenzene allows for the rebinding of cyclodextrin and resealing of the nanopores, a process which entraps the remaining cargo. Two stalks were designed with different lengths and tested with alizarin red S and propidium iodide. No cargo release was observed prior to light irradiation, and the system was capable of multiuse. On / off control was also demonstrated by monitoring the release of cargo when the light stimulus was applied and removed, respectively. PMID:24519642

An Airbus arrives at KSC with third MPLM

NASA Technical Reports Server (NTRS)

2001-01-01

An Airbus '''Beluga''' air cargo plane, The Super Transporter, taxis onto the parking apron at KSC's Shuttle Landing Facility. Its cargo, from the factory of Alenia Aerospazio in Turin, Italy, is the Italian Space Agency's Multi-Purpose Logistics Module Donatello, the third of three for the International Space Station. The module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

KSC01pp0234

NASA Image and Video Library

2001-02-01

An Airbus “Beluga” air cargo plane, The Super Transporter, taxis onto the parking apron at KSC’s Shuttle Landing Facility. Its cargo, from the factory of Alenia Aerospazio in Turin, Italy, is the Italian Space Agency’s Multi-Purpose Logistics Module Donatello, the third of three for the International Space Station. The module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle’s payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo

Cosmic Ray Inspection and Passive Tomography for SNM Detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Armitage, John; Oakham, Gerald; Bryman, Douglas

2009-12-02

The Cosmic Ray Inspection and Passive Tomography (CRIPT) project has recently started investigating the detection of illicit Special Nuclear Material in cargo using cosmic ray muon tomography and complementary neutron detectors. We are currently performing simulation studies to help with the design of small scale prototypes. Based on the prototype tests and refined simulations, we will determine whether the muon tracking system for the full scale prototype will be based on drift chambers or extruded scintillator trackers. An analysis of the operations of the Port of Montreal has determined how long muon scan times should take if all or amore » subset of the cargo is to be screened. As long as the throughput of the muon system(s) is equal to the rate at which containers are unloaded from ships, the impact on port operations would not be great if a muon scanning stage were required for all cargo. We also show preliminary simulation results indicating that excellent separation between Al, Fe and Pb is possible under ideal conditions. The discrimination power is reduced but still significant when realistic momentum resolution measurements are considered.« less

Cosmic Ray Inspection and Passive Tomography for SNM Detection

NASA Astrophysics Data System (ADS)

Armitage, John; Bryman, Douglas; Cousins, Thomas; Gallant, Grant; Jason, Andrew; Jonkmans, Guy; Noël, Scott; Oakham, Gerald; Stocki, Trevor J.; Waller, David

2009-12-01

The Cosmic Ray Inspection and Passive Tomography (CRIPT) project has recently started investigating the detection of illicit Special Nuclear Material in cargo using cosmic ray muon tomography and complementary neutron detectors. We are currently performing simulation studies to help with the design of small scale prototypes. Based on the prototype tests and refined simulations, we will determine whether the muon tracking system for the full scale prototype will be based on drift chambers or extruded scintillator trackers. An analysis of the operations of the Port of Montreal has determined how long muon scan times should take if all or a subset of the cargo is to be screened. As long as the throughput of the muon system(s) is equal to the rate at which containers are unloaded from ships, the impact on port operations would not be great if a muon scanning stage were required for all cargo. We also show preliminary simulation results indicating that excellent separation between Al, Fe and Pb is possible under ideal conditions. The discrimination power is reduced but still significant when realistic momentum resolution measurements are considered.

Dynamic optimization of cargo movement by trucks in metropolitan areas with adjacent ports

DOT National Transportation Integrated Search

2002-06-01

Today, in the trucking industry, dispatchers perform the tasks of cargo assignment, and driver scheduling. The growing number of containers processed at marine centers and the increasing traffic congestion in metropolitan areas adjacent to marine por...

Cargo-Delivery Platforms for Targeted Delivery of Inhibitor Cargos Against Botulism

PubMed Central

Wilson, Brenda A.; Ho, Mengfei

2015-01-01

Delivering therapeutic cargos to specific cell types in vivo poses many technical challenges. There is currently a plethora of drug leads and therapies against numerous diseases, ranging from small molecule compounds to nucleic acids to peptides to proteins with varying binding or enzymatic functions. Many of these candidate therapies have documented potential for mitigating or reversing disease symptoms, if only a means for gaining access to the intracellular target were available. Recent advances in our understanding of the biology of cellular uptake and transport processes and the mode of action of bacterial protein toxins have accelerated the development of toxin-based cargo-delivery vehicle platforms. This review provides an updated survey of the status of available platforms for targeted delivery of therapeutic cargos, outlining various strategies that have been used to deliver different types of cargo into cells. Particular emphasis is placed on the application of toxin-based approaches, examining critical issues that have hampered realization of post-intoxication antitoxins against botulism. PMID:25335885

Cargo-delivery platforms for targeted delivery of inhibitor cargos against botulism.

PubMed

Wilson, Brenda A; Ho, Mengfei

2014-01-01

Delivering therapeutic cargos to specific cell types in vivo poses many technical challenges. There is currently a plethora of drug leads and therapies against numerous diseases, ranging from small molecule compounds to nucleic acids to peptides to proteins with varying binding or enzymatic functions. Many of these candidate therapies have documented potential for mitigating or reversing disease symptoms, if only a means for gaining access to the intracellular target were available. Recent advances in our understanding of the biology of cellular uptake and transport processes and the mode of action of bacterial protein toxins have accelerated the development of toxin-based cargo-delivery vehicle platforms. This review provides an updated survey of the status of available platforms for targeted delivery of therapeutic cargos, outlining various strategies that have been used to deliver different types of cargo into cells. Particular emphasis is placed on the application of toxin-based approaches, examining critical issues that have hampered realization of post-intoxication antitoxins against botulism.

46 CFR 151.50-76 - Hydrochloric acid, spent (NTE 15%).

Code of Federal Regulations, 2010 CFR

2010-10-01

... not be less than three-eighths inch. A shell plating of a barge may not be on the boundary of any part... obstructions and away from any from any source of ignition; and (ii) Be fitted with a single flame screen or... prohibited and the person in charge of cargo transfer shall ensure that “No Smoking” signs are displayed...

GFP-complementation assay to detect functional CPP and protein delivery into living cells

PubMed Central

Milech, Nadia; Longville, Brooke AC; Cunningham, Paula T; Scobie, Marie N; Bogdawa, Heique M; Winslow, Scott; Anastasas, Mark; Connor, Theresa; Ong, Ferrer; Stone, Shane R; Kerfoot, Maria; Heinrich, Tatjana; Kroeger, Karen M; Tan, Yew-Foon; Hoffmann, Katrin; Thomas, Wayne R; Watt, Paul M; Hopkins, Richard M

2015-01-01

Efficient cargo uptake is essential for cell-penetrating peptide (CPP) therapeutics, which deliver widely diverse cargoes by exploiting natural cell processes to penetrate the cell’s membranes. Yet most current CPP activity assays are hampered by limitations in assessing uptake, including confounding effects of conjugated fluorophores or ligands, indirect read-outs requiring secondary processing, and difficulty in discriminating internalization from endosomally trapped cargo. Split-complementation Endosomal Escape (SEE) provides the first direct assay visualizing true cytoplasmic-delivery of proteins at biologically relevant concentrations. The SEE assay has minimal background, is amenable to high-throughput processes, and adaptable to different transient and stable cell lines. This split-GFP-based platform can be useful to study transduction mechanisms, cellular imaging, and characterizing novel CPPs as pharmaceutical delivery agents in the treatment of disease. PMID:26671759

REEPs Are Membrane Shaping Adapter Proteins That Modulate Specific G Protein-Coupled Receptor Trafficking by Affecting ER Cargo Capacity

PubMed Central

Ho, Vincent K.; Angelotti, Timothy

2013-01-01

Receptor expression enhancing proteins (REEPs) were identified by their ability to enhance cell surface expression of a subset of G protein-coupled receptors (GPCRs), specifically GPCRs that have proven difficult to express in heterologous cell systems. Further analysis revealed that they belong to the Yip (Ypt-interacting protein) family and that some REEP subtypes affect ER structure. Yip family comparisons have established other potential roles for REEPs, including regulation of ER-Golgi transport and processing/neuronal localization of cargo proteins. However, these other potential REEP functions and the mechanism by which they selectively enhance GPCR cell surface expression have not been clarified. By utilizing several REEP family members (REEP1, REEP2, and REEP6) and model GPCRs (α2A and α2C adrenergic receptors), we examined REEP regulation of GPCR plasma membrane expression, intracellular processing, and trafficking. Using a combination of immunolocalization and biochemical methods, we demonstrated that this REEP subset is localized primarily to ER, but not plasma membranes. Single cell analysis demonstrated that these REEPs do not specifically enhance surface expression of all GPCRs, but affect ER cargo capacity of specific GPCRs and thus their surface expression. REEP co-expression with α2 adrenergic receptors (ARs) revealed that this REEP subset interacts with and alter glycosidic processing of α2C, but not α2A ARs, demonstrating selective interaction with cargo proteins. Specifically, these REEPs enhanced expression of and interacted with minimally/non-glycosylated forms of α2C ARs. Most importantly, expression of a mutant REEP1 allele (hereditary spastic paraplegia SPG31) lacking the carboxyl terminus led to loss of this interaction. Thus specific REEP isoforms have additional intracellular functions besides altering ER structure, such as enhancing ER cargo capacity, regulating ER-Golgi processing, and interacting with select cargo proteins. Therefore, some REEPs can be further described as ER membrane shaping adapter proteins. PMID:24098485

Turnover of Lipidated LC3 and Autophagic Cargoes in Mammalian Cells.

PubMed

Rodríguez-Arribas, M; Yakhine-Diop, S M S; González-Polo, R A; Niso-Santano, M; Fuentes, J M

2017-01-01

Macroautophagy (usually referred to as autophagy) is the most important degradation system in mammalian cells. It is responsible for the elimination of protein aggregates, organelles, and other cellular content. During autophagy, these materials (i.e., cargo) must be engulfed by a double-membrane structure called an autophagosome, which delivers the cargo to the lysosome to complete its degradation. Autophagy is a very dynamic pathway called autophagic flux. The process involves all the steps that are implicated in cargo degradation from autophagosome formation. There are several techniques to monitor autophagic flux. Among them, the method most used experimentally to assess autophagy is the detection of LC3 protein processing and p62 degradation by Western blotting. In this chapter, we provide a detailed and straightforward protocol for this purpose in cultured mammalian cells, including a brief set of notes concerning problems associated with the Western-blotting detection of LC3 and p62. © 2017 Elsevier Inc. All rights reserved.

Dendritic polymer imaging systems for the evaluation of conjugate uptake and cleavage

NASA Astrophysics Data System (ADS)

Krüger, Harald R.; Nagel, Gregor; Wedepohl, Stefanie; Calderón, Marcelo

2015-02-01

Fluorescent turn-on probes combined with polymers have a broad range of applications, e.g. for intracellular sensing of ions, small molecules, or DNA. In the field of polymer therapeutics, these probes can be applied to extend the in vitro characterization of novel conjugates beyond cytotoxicity and cellular uptake studies. This is particularly true in cases in which polymer conjugates contain drugs attached by cleavable linkers. Better information on the intracellular linker cleavage and drug release would allow a faster evaluation and optimization of novel polymer therapeutic concepts. We therefore developed a fluorescent turn-on probe that enables direct monitoring of pH-mediated cleavage processes over time. This is achieved by exploiting the fluorescence resonance energy transfer (FRET) between two dyes that have been coupled to a dendritic polymer. We demonstrate the use of this probe to evaluate polymer uptake and intracellular release of cargo in a cell based microplate assay that is suitable for high throughput screening.Fluorescent turn-on probes combined with polymers have a broad range of applications, e.g. for intracellular sensing of ions, small molecules, or DNA. In the field of polymer therapeutics, these probes can be applied to extend the in vitro characterization of novel conjugates beyond cytotoxicity and cellular uptake studies. This is particularly true in cases in which polymer conjugates contain drugs attached by cleavable linkers. Better information on the intracellular linker cleavage and drug release would allow a faster evaluation and optimization of novel polymer therapeutic concepts. We therefore developed a fluorescent turn-on probe that enables direct monitoring of pH-mediated cleavage processes over time. This is achieved by exploiting the fluorescence resonance energy transfer (FRET) between two dyes that have been coupled to a dendritic polymer. We demonstrate the use of this probe to evaluate polymer uptake and intracellular release of cargo in a cell based microplate assay that is suitable for high throughput screening. Electronic supplementary information (ESI) available: Including detailed synthetic procedures of the dye and conjugate synthesis, as well as cellular uptake and inhibitor studies. See DOI: 10.1039/c4nr04467c

MODELING AND PERFORMANCE EVALUATION FOR AVIATION SECURITY CARGO INSPECTION QUEUING SYSTEM

DOE Office of Scientific and Technical Information (OSTI.GOV)

Allgood, Glenn O; Olama, Mohammed M; Rose, Terri A

Beginning in 2010, the U.S. will require that all cargo loaded in passenger aircraft be inspected. This will require more efficient processing of cargo and will have a significant impact on the inspection protocols and business practices of government agencies and the airlines. In this paper, we conduct performance evaluation study for an aviation security cargo inspection queuing system for material flow and accountability. The overall performance of the aviation security cargo inspection system is computed, analyzed, and optimized for the different system dynamics. Various performance measures are considered such as system capacity, residual capacity, and throughput. These metrics aremore » performance indicators of the system s ability to service current needs and response capacity to additional requests. The increased physical understanding resulting from execution of the queuing model utilizing these vetted performance measures will reduce the overall cost and shipping delays associated with the new inspection requirements.« less

Endocytosis and Endosomal Trafficking in Plants.

PubMed

Paez Valencia, Julio; Goodman, Kaija; Otegui, Marisa S

2016-04-29

Endocytosis and endosomal trafficking are essential processes in cells that control the dynamics and turnover of plasma membrane proteins, such as receptors, transporters, and cell wall biosynthetic enzymes. Plasma membrane proteins (cargo) are internalized by endocytosis through clathrin-dependent or clathrin-independent mechanism and delivered to early endosomes. From the endosomes, cargo proteins are recycled back to the plasma membrane via different pathways, which rely on small GTPases and the retromer complex. Proteins that are targeted for degradation through ubiquitination are sorted into endosomal vesicles by the ESCRT (endosomal sorting complex required for transport) machinery for degradation in the vacuole. Endocytic and endosomal trafficking regulates many cellular, developmental, and physiological processes, including cellular polarization, hormone transport, metal ion homeostasis, cytokinesis, pathogen responses, and development. In this review, we discuss the mechanisms that mediate the recognition and sorting of endocytic and endosomal cargos, the vesiculation processes that mediate their trafficking, and their connection to cellular and physiological responses in plants.

Integration of Synaptic Vesicle Cargo Retrieval with Endocytosis at Central Nerve Terminals

PubMed Central

Cousin, Michael A.

2017-01-01

Central nerve terminals contain a limited number of synaptic vesicles (SVs) which mediate the essential process of neurotransmitter release during their activity-dependent fusion. The rapid and accurate formation of new SVs with the appropriate cargo is essential to maintain neurotransmission in mammalian brain. Generating SVs containing the correct SV cargo with the appropriate stoichiometry is a significant challenge, especially when multiple modes of endocytosis exist in central nerve terminals, which occur at different locations within the nerve terminals. These endocytosis modes include ultrafast endocytosis, clathrin-mediated endocytosis (CME) and activity-dependent bulk endocytosis (ADBE) which are triggered by specific patterns of neuronal activity. This review article will assess the evidence for the role of classical adaptor protein complexes in SV retrieval, discuss the role of monomeric adaptors and how interactions between specific SV cargoes can facilitate retrieval. In addition it will consider the evidence for preassembled plasma membrane cargo complexes and their role in facilitating these endocytosis modes. Finally it will present a unifying model for cargo retrieval at the presynapse, which integrates endocytosis modes in time and space. PMID:28824381

Bacterial Swarms Recruit Cargo Bacteria To Pave the Way in Toxic Environments

PubMed Central

Finkelshtein, Alin; Roth, Dalit

2015-01-01

ABSTRACT Swarming bacteria are challenged by the need to invade hostile environments. Swarms of the flagellated bacterium Paenibacillus vortex can collectively transport other microorganisms. Here we show that P. vortex can invade toxic environments by carrying antibiotic-degrading bacteria; this transport is mediated by a specialized, phenotypic subpopulation utilizing a process not dependent on cargo motility. Swarms of beta-lactam antibiotic (BLA)-sensitive P. vortex used beta-lactamase-producing, resistant, cargo bacteria to detoxify BLAs in their path. In the presence of BLAs, both transporter and cargo bacteria gained from this temporary cooperation; there was a positive correlation between BLA resistance and dispersal. P. vortex transported only the most beneficial antibiotic-resistant cargo (including environmental and clinical isolates) in a sustained way. P. vortex displayed a bet-hedging strategy that promoted the colonization of nontoxic niches by P. vortex alone; when detoxifying cargo bacteria were not needed, they were lost. This work has relevance for the dispersal of antibiotic-resistant microorganisms and for strategies for asymmetric cooperation with agricultural and medical implications. PMID:25968641

Drive the Car(go)s-New Modalities to Control Cargo Trafficking in Live Cells.

PubMed

Mondal, Payel; Khamo, John S; Krishnamurthy, Vishnu V; Cai, Qi; Zhang, Kai

2017-01-01

Synaptic transmission is a fundamental molecular process underlying learning and memory. Successful synaptic transmission involves coupled interaction between electrical signals (action potentials) and chemical signals (neurotransmitters). Defective synaptic transmission has been reported in a variety of neurological disorders such as Autism and Alzheimer's disease. A large variety of macromolecules and organelles are enriched near functional synapses. Although a portion of macromolecules can be produced locally at the synapse, a large number of synaptic components especially the membrane-bound receptors and peptide neurotransmitters require active transport machinery to reach their sites of action. This spatial relocation is mediated by energy-consuming, motor protein-driven cargo trafficking. Properly regulated cargo trafficking is of fundamental importance to neuronal functions, including synaptic transmission. In this review, we discuss the molecular machinery of cargo trafficking with emphasis on new experimental strategies that enable direct modulation of cargo trafficking in live cells. These strategies promise to provide insights into a quantitative understanding of cargo trafficking, which could lead to new intervention strategies for the treatment of neurological diseases.

Combining Radiography and Passive Measurements for Radiological Threat Detection in Cargo

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, Erin A.; White, Timothy A.; Jarman, Kenneth D.

Abstract Radiography is widely understood to provide information complimentary to passive detection: while not directly sensitive to radiological materials, radiography can reveal highly shielded regions which may mask a passive radiological signal. We present a method for combining radiographic and passive data which uses the radiograph to provide an estimate of scatter and attenuation for possible sources. This approach allows quantitative use of radiographic images without relying on image interpretation, and results in a probabilistic description of likely source locations and strengths. We present first results for this method for a simple modeled test case of a cargo container drivingmore » through a PVT portal. With this inversion approach, we address criteria for an integrated passive and radiographic screening system and how detection of SNM threats might be improved in such a system.« less

An Improved Spectral Analysis Method for Fatigue Damage Assessment of Details in Liquid Cargo Tanks

NASA Astrophysics Data System (ADS)

Zhao, Peng-yuan; Huang, Xiao-ping

2018-03-01

Errors will be caused in calculating the fatigue damages of details in liquid cargo tanks by using the traditional spectral analysis method which is based on linear system, for the nonlinear relationship between the dynamic stress and the ship acceleration. An improved spectral analysis method for the assessment of the fatigue damage in detail of a liquid cargo tank is proposed in this paper. Based on assumptions that the wave process can be simulated by summing the sinusoidal waves in different frequencies and the stress process can be simulated by summing the stress processes induced by these sinusoidal waves, the stress power spectral density (PSD) is calculated by expanding the stress processes induced by the sinusoidal waves into Fourier series and adding the amplitudes of each harmonic component with the same frequency. This analysis method can take the nonlinear relationship into consideration and the fatigue damage is then calculated based on the PSD of stress. Take an independent tank in an LNG carrier for example, the accuracy of the improved spectral analysis method is proved much better than that of the traditional spectral analysis method by comparing the calculated damage results with the results calculated by the time domain method. The proposed spectral analysis method is more accurate in calculating the fatigue damages in detail of ship liquid cargo tanks.

MPLM Donatello is offloaded at the SLF

NASA Technical Reports Server (NTRS)

2001-01-01

At the Shuttle Landing Facility, cranes help offload the Italian Space Agency's Multi-Purpose Logistics Module Donatello from the Airbus '''Beluga''' air cargo plane. The third of three for the International Space Station, the module will be moved on a transporter to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

Quantifying the Dynamic Interactions Between a Clathrin-Coated Pit and Cargo Molecules

NASA Astrophysics Data System (ADS)

Weigel, Aubrey; Tamkun, Michael; Krapf, Diego

2014-03-01

Clathrin-mediated endocytosis is a major pathway of internalization of cargo in eukaryotic cells. This process involves the recruitment of cargo molecules into a growing clathrin-coated pit (CCP). However, cargo-CCP interactions are difficult to study because CCPs display a large degree of lifetime heterogeneity and the interactions with cargo molecules evolve over time. We use single-molecule total internal reflection fluorescence (TIRF) microscopy, in combination with automatic detection and tracking algorithms, to directly visualize the recruitment of individual voltage-gated potassium channels into forming CCPs in living cells. Contrary to widespread ideas, cargo often escapes from a pit before abortive CCP termination or endocytic vesicle production. By measuring tens of thousands of capturing events, we build the distribution of capture times and the times that cargo remains confined to a CCP. An analytical stochastic model is developed and compared to the measured distributions. Due to the dynamic nature of the pit, the model is non-Markovian and it displays long-tail power law statistics. Our findings identify one source of the large heterogeneities observed in CCP maturation and provide a mechanism for the anomalous diffusion of proteins in the plasma membrane. This work was supported by National Science Foundation Grant PHY-0956714.

40 CFR 63.2406 - What definitions apply to this subpart?

Code of Federal Regulations, 2012 CFR

2012-07-01

.... Examples of containers include, but are not limited to, drums and portable cargo containers known as... language in § 63.921, the terms “container” and “safety device” shall have the meaning found in this... storage or for further processing downstream. Cargo tank means a liquid-carrying tank permanently attached...

MPLM Donatello is offloaded at the SLF

NASA Technical Reports Server (NTRS)

2001-01-01

At the KSC Shuttle Landing Facility, an Airbus '''Beluga''' air cargo plane opens to reveal its cargo, the Italian Space Agency's Multi- Purpose Logistics Module Donatello, from the factory of Alenia Aerospazio in Turin, Italy. The third of three for the International Space Station, the module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

The Guardian. Volume 9, Number 2, Fall 2007

DTIC Science & Technology

2007-01-01

Working Group, the National Institute of Occupational Safety and Health , the National Institute of Justice, the National Fire Protection Association...protection under the SAFETY Act. The CEO refused to resubmit the bid without the caveat. He stated, “I can’t bet my corporation on the possibility...guide drivers to the inspection site, which can include under -vehicle screening and cargo inspection systems. Vehicle occupants will be directed to

Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables

PubMed Central

Clayton, Joseph E.; Pollard, Luther W.; Sckolnick, Maria; Bookwalter, Carol S.; Hodges, Alex R.; Trybus, Kathleen M.; Lord, Matthew

2014-01-01

A hallmark of class-V myosins is their processivity—the ability to take multiple steps along actin filaments without dissociating. Our previous work suggested, however, that the fission yeast myosin-V (Myo52p) is a nonprocessive motor whose activity is enhanced by tropomyosin (Cdc8p). Here we investigate the molecular mechanism and physiological relevance of tropomyosin-mediated regulation of Myo52p transport, using a combination of in vitro and in vivo approaches. Single molecules of Myo52p, visualized by total internal reflection fluorescence microscopy, moved processively only when Cdc8p was present on actin filaments. Small ensembles of Myo52p bound to a quantum dot, mimicking the number of motors bound to physiological cargo, also required Cdc8p for continuous motion. Although a truncated form of Myo52p that lacked a cargo-binding domain failed to support function in vivo, it still underwent actin-dependent movement to polarized growth sites. This result suggests that truncated Myo52p lacking cargo, or single molecules of wild-type Myo52p with small cargoes, can undergo processive movement along actin-Cdc8p cables in vivo. Our findings outline a mechanism by which tropomyosin facilitates sorting of transport to specific actin tracks within the cell by switching on myosin processivity. PMID:24196839

Versatile Loading of Diverse Cargo into Functional Polymer Capsules.

PubMed

Richardson, Joseph J; Maina, James W; Ejima, Hirotaka; Hu, Ming; Guo, Junling; Choy, Mei Y; Gunawan, Sylvia T; Lybaert, Lien; Hagemeyer, Christoph E; De Geest, Bruno G; Caruso, Frank

2015-02-01

Polymer microcapsules are of particular interest for applications including self-healing coatings, catalysis, bioreactions, sensing, and drug delivery. The primary way that polymer capsules can exhibit functionality relevant to these diverse fields is through the incorporation of functional cargo in the capsule cavity or wall. Diverse functional and therapeutic cargo can be loaded into polymer capsules with ease using polymer-stabilized calcium carbonate (CaCO 3 ) particles. A variety of examples are demonstrated, including 15 types of cargo, yielding a toolbox with effectively 500+ variations. This process uses no harsh reagents and can take less than 30 min to prepare, load, coat, and form the hollow capsules. For these reasons, it is expected that the technique will play a crucial role across scientific studies in numerous fields.

Structural Insights into Functional Overlapping and Differentiation among Myosin V Motors*

PubMed Central

Nascimento, Andrey F. Z.; Trindade, Daniel M.; Tonoli, Celisa C. C.; de Giuseppe, Priscila O.; Assis, Leandro H. P.; Honorato, Rodrigo V.; de Oliveira, Paulo S. L.; Mahajan, Pravin; Burgess-Brown, Nicola A.; von Delft, Frank; Larson, Roy E.; Murakami, Mario T.

2013-01-01

Myosin V (MyoV) motors have been implicated in the intracellular transport of diverse cargoes including vesicles, organelles, RNA-protein complexes, and regulatory proteins. Here, we have solved the cargo-binding domain (CBD) structures of the three human MyoV paralogs (Va, Vb, and Vc), revealing subtle structural changes that drive functional differentiation and a novel redox mechanism controlling the CBD dimerization process, which is unique for the MyoVc subclass. Moreover, the cargo- and motor-binding sites were structurally assigned, indicating the conservation of residues involved in the recognition of adaptors for peroxisome transport and providing high resolution insights into motor domain inhibition by CBD. These results contribute to understanding the structural requirements for cargo transport, autoinhibition, and regulatory mechanisms in myosin V motors. PMID:24097982

Sliding of microtubules by a team of dynein motors: Understanding the effect of spatial distribution of motor tails and mutual exclusion of motor heads on microtubules

NASA Astrophysics Data System (ADS)

Singh, Hanumant Pratap; Takshak, Anjneya; Mall, Utkarsh; Kunwar, Ambarish

2016-06-01

Molecular motors are natural nanomachines that use the free energy released from ATP hydrolysis to generate mechanical forces. Cytoplasmic dynein motors often work collectively as a team to drive important processes such as axonal growth, proplatelet formation and mitosis, as forces generated by single motors are insufficient. A large team of dynein motors is used to slide cytoskeletal microtubules with respect to one another during the process of proplatelet formation and axonal growth. These motors attach to a cargo microtubule via their tail domains, undergo the process of detachment and reattachment of their head domains on another track microtubule, while sliding the cargo microtubule along the track. Traditional continuum/mean-field approaches used in the past are not ideal for studying the sliding mechanism of microtubules, as they ignore spatial and temporal fluctuations due to different possible distributions of motor tails on cargo filament, as well as binding/unbinding of motors from their track. Therefore, these models cannot be used to address important questions such as how the distribution of motor tails on microtubules, or how the mutual exclusion of motor heads on microtubule tracks affects the sliding velocity of cargo microtubule. To answer these, here we use a computational stochastic model where we model each dynein motor explicitly. In our model, we use both random as well as uniform distributions of dynein motors on cargo microtubule, as well as mutual exclusion of motors on microtubule tracks. We find that sliding velocities are least affected by the distribution of motor tails on microtubules, whereas they are greatly affected by mutual exclusion of motor heads on microtubule tracks. We also find that sliding velocity depends on the length of cargo microtubule if mutual exclusion among motor heads is considered.

Chemically active reduced graphene oxide with tunable C/O ratios.

PubMed

Compton, Owen C; Jain, Bonny; Dikin, Dmitriy A; Abouimrane, Ali; Amine, Khalil; Nguyen, Sonbinh T

2011-06-28

Organic dispersions of graphene oxide can be thermally reduced in polar organic solvents under reflux conditions to afford electrically conductive, chemically active reduced graphene oxide (CARGO) with tunable C/O ratios, dependent on the boiling point of the solvent. The reductions are achieved after only 1 h of reflux, and the corresponding C/O ratios do not change upon further thermal treatment. Hydroxyl and carboxyl groups can be removed when the reflux is carried out above 155 °C, while epoxides are removable only when the temperature is higher than 200 °C. The increasing hydrophobic nature of CARGO, as its C/O ratio increases, improves the dispersibility of the nanosheets in a polystyrene matrix, in contrast to the aggregates formed with CARGO having lower C/O ratios. The excellent processability of the obtained CARGO dispersions is demonstrated via free-standing CARGO papers that exhibit tunable electrical conductivity/chemical activity and can be used as lithium-ion battery anodes with enhanced Coulombic efficiency.

Cargo binding promotes KDEL receptor clustering at the mammalian cell surface

PubMed Central

Becker, Björn; Shaebani, M. Reza; Rammo, Domenik; Bubel, Tobias; Santen, Ludger; Schmitt, Manfred J.

2016-01-01

Transmembrane receptor clustering is a ubiquitous phenomenon in pro- and eukaryotic cells to physically sense receptor/ligand interactions and subsequently translate an exogenous signal into a cellular response. Despite that receptor cluster formation has been described for a wide variety of receptors, ranging from chemotactic receptors in bacteria to growth factor and neurotransmitter receptors in mammalian cells, a mechanistic understanding of the underlying molecular processes is still puzzling. In an attempt to fill this gap we followed a combined experimental and theoretical approach by dissecting and modulating cargo binding, internalization and cellular response mediated by KDEL receptors (KDELRs) at the mammalian cell surface after interaction with a model cargo/ligand. Using a fluorescent variant of ricin toxin A chain as KDELR-ligand (eGFP-RTAH/KDEL), we demonstrate that cargo binding induces dose-dependent receptor cluster formation at and subsequent internalization from the membrane which is associated and counteracted by anterograde and microtubule-assisted receptor transport to preferred docking sites at the plasma membrane. By means of analytical arguments and extensive numerical simulations we show that cargo-synchronized receptor transport from and to the membrane is causative for KDELR/cargo cluster formation at the mammalian cell surface. PMID:27353000

Cargo binding promotes KDEL receptor clustering at the mammalian cell surface

NASA Astrophysics Data System (ADS)

Becker, Björn; Shaebani, M. Reza; Rammo, Domenik; Bubel, Tobias; Santen, Ludger; Schmitt, Manfred J.

2016-06-01

Transmembrane receptor clustering is a ubiquitous phenomenon in pro- and eukaryotic cells to physically sense receptor/ligand interactions and subsequently translate an exogenous signal into a cellular response. Despite that receptor cluster formation has been described for a wide variety of receptors, ranging from chemotactic receptors in bacteria to growth factor and neurotransmitter receptors in mammalian cells, a mechanistic understanding of the underlying molecular processes is still puzzling. In an attempt to fill this gap we followed a combined experimental and theoretical approach by dissecting and modulating cargo binding, internalization and cellular response mediated by KDEL receptors (KDELRs) at the mammalian cell surface after interaction with a model cargo/ligand. Using a fluorescent variant of ricin toxin A chain as KDELR-ligand (eGFP-RTAH/KDEL), we demonstrate that cargo binding induces dose-dependent receptor cluster formation at and subsequent internalization from the membrane which is associated and counteracted by anterograde and microtubule-assisted receptor transport to preferred docking sites at the plasma membrane. By means of analytical arguments and extensive numerical simulations we show that cargo-synchronized receptor transport from and to the membrane is causative for KDELR/cargo cluster formation at the mammalian cell surface.

A shuttle and space station manipulator system for assembly, docking, maintenance, cargo handling and spacecraft retrieval (preliminary design). Volume 3: Concept analysis. Part 1: Technical

NASA Technical Reports Server (NTRS)

1972-01-01

Information backing up the key features of the manipulator system concept and detailed technical information on the subsystems are presented. Space station assembly and shuttle cargo handling tasks are emphasized in the concept analysis because they involve shuttle berthing, transferring the manipulator boom between shuttle and station, station assembly, and cargo handling. Emphasis is also placed on maximizing commonality in the system areas of manipulator booms, general purpose end effectors, control and display, data processing, telemetry, dedicated computers, and control station design.

Science and Technology Review May 2004

DOE Office of Scientific and Technical Information (OSTI.GOV)

Henson, V E

2004-04-02

This month's issue has the following articles: (1) ''A Multidisciplinary Attack on Bioterrorism''--Commentary by Tomas Diaz de la Rubia. (2) ''Life at the Nanoscale''--Livermore researchers are exploring the molecules of life at the nanoscale to fight bioterrorism, improve human health, and understand how proteins function. (3) ''Screening Cargo Containers to Remove a Terrorist Threat''--A detection system for cargo containers will search for hidden nuclear materials that terrorists might try to ship to U.S. seaports. (4) ''Improved Algorithms Speed It Up for Codes''--Recent changes to the algorithms used in Monte Carlo calculations are reducing the time needed to run the high-fidelitymore » simulations being developed for the nation's supercomputers. (5) ''The Siren Call of the Seas: Sequestering Carbon Dioxide''--Scientists at Livermore evaluate possible methods for removing carbon dioxide from Earth's atmosphere and storing it in the planet's waters.« less

Water Flowing on Mars Today on This Week @NASA – October 2, 2015

NASA Image and Video Library

2015-10-02

A major scientific discovery was announced by NASA at a Sept. 28 news conference. From its vantage point high above the Martian surface, NASA’s Mars Reconnaissance Orbiter (MRO) spacecraft has found the strongest evidence yet, that under certain circumstances, liquid water has been found on Mars. Researchers say an imaging spectrometer on MRO detected signatures of hydrated minerals on slopes where downhill streaks, known as Recurring Slope Lineae (RSL) are seen. In the past, RSL flows have been described as possibly related to liquid water. But the new findings of hydrated minerals is key evidence. Hydrated salts can lower the freezing point of liquid brine – and produce liquid water. Also, Life beyond Earth in the next decade?, “The Martian” screening event, Cargo ship departs space station, New cargo ship delivers to space station, Rare double celestial treat and Espacio a Tierra!

Two Novel Rab2 Interactors Regulate Dense-core Vesicle Maturation

PubMed Central

Ailion, Michael; Hannemann, Mandy; Dalton, Susan; Pappas, Andrea; Watanabe, Shigeki; Hegermann, Jan; Liu, Qiang; Han, Hsiao-Fen; Gu, Mingyu; Goulding, Morgan Q.; Sasidharan, Nikhil; Schuske, Kim; Hullett, Patrick; Eimer, Stefan; Jorgensen, Erik M.

2014-01-01

Summary Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi, and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1 and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a new pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network. PMID:24698274

46 CFR 154.506 - Mechanical expansion joint: Limits in a piping system.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Mechanical expansion joint: Limits in a piping system..., Construction and Equipment Cargo and Process Piping Systems § 154.506 Mechanical expansion joint: Limits in a piping system. Mechanical expansion joints in a piping system outside of a cargo tank: (a) May be...

46 CFR 154.506 - Mechanical expansion joint: Limits in a piping system.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Mechanical expansion joint: Limits in a piping system..., Construction and Equipment Cargo and Process Piping Systems § 154.506 Mechanical expansion joint: Limits in a piping system. Mechanical expansion joints in a piping system outside of a cargo tank: (a) May be...

46 CFR 154.506 - Mechanical expansion joint: Limits in a piping system.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Mechanical expansion joint: Limits in a piping system..., Construction and Equipment Cargo and Process Piping Systems § 154.506 Mechanical expansion joint: Limits in a piping system. Mechanical expansion joints in a piping system outside of a cargo tank: (a) May be...

46 CFR 154.506 - Mechanical expansion joint: Limits in a piping system.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Mechanical expansion joint: Limits in a piping system..., Construction and Equipment Cargo and Process Piping Systems § 154.506 Mechanical expansion joint: Limits in a piping system. Mechanical expansion joints in a piping system outside of a cargo tank: (a) May be...

46 CFR 154.506 - Mechanical expansion joint: Limits in a piping system.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Mechanical expansion joint: Limits in a piping system..., Construction and Equipment Cargo and Process Piping Systems § 154.506 Mechanical expansion joint: Limits in a piping system. Mechanical expansion joints in a piping system outside of a cargo tank: (a) May be...

The tetraspanin CD63 regulates ESCRT-independent and dependent endosomal sorting during melanogenesis

PubMed Central

van Niel, Guillaume; Charrin, Stéphanie; Simoes, Sabrina; Romao, Maryse; Rochin, Leila; Saftig, Paul; Marks, Michael S.; Rubinstein, Eric; Raposo, Graça

2011-01-01

Summary Cargo sorting to intraluminal vesicles (ILVs) of multivesicular endosomes is required for numerous physiological processes including lysosome-related organelle (LRO) biogenesis. PMEL – a component of melanocyte LROs (melanosomes) – is sorted to ILVs in an ESCRT-independent manner, where it is proteolytically processed and assembled into functional amyloid fibrils during melanosome maturation. Here we show that the tetraspanin CD63 directly participates in ESCRT-independent sorting of the PMEL luminal domain, but not of traditional ESCRT-dependent cargoes, to ILVs. Inactivating CD63 in cell culture or in mice impairs amyloidogenesis and downstream melanosome morphogenesis. Whereas CD63 is required for normal PMEL luminal domain sorting, the disposal of the remaining PMEL transmembrane fragment requires functional ESCRTs but not CD63. In the absence of CD63, the PMEL luminal domain follows this fragment and is targeted for ESCRT-dependent degradation. Our data thus reveal a tight interplay regulated by CD63 between two distinct endosomal ILV sorting processes for a single cargo during LRO biogenesis. PMID:21962903

ORNL Trusted Corridors Project: Watts Bar Dam Inland Waterway Project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walker, Randy M; Gross, Ian G; Smith, Cyrus M

2011-11-01

Radiation has existed everywhere in the environment since the Earth's formation - in rocks, soil, water, and plants. The mining and processing of naturally occurring radioactive materials for use in medicine, power generation, consumer products, and industry inevitably generate emissions and waste. Radiological measuring devices have been used by industry for years to measure for radiation in undesired locations or simply identify radioactive materials. Since the terrorist attacks on the United States on 9-11-01 these radiation measuring devices have proliferated in many places in our nation's commerce system. DOE, TVA, the Army Corps and ORNL collaborated to test the usefulnessmore » of these devices in our nation's waterway system on this project. The purpose of the Watts Bar Dam ORNL Trusted Corridors project was to investigate the security, safety and enforcement needs of local, state and federal government entities for state-of-the-art sensor monitoring in regards to illegal cargo including utilization of the existing infrastructure. TVA's inland waterways lock system is a recognized and accepted infrastructure by the commercial carrier industry. Safety Monitoring activities included tow boat operators, commercial barges and vessels, recreational watercraft and their cargo, identification of unsafe vessels and carriers, and, monitoring of domestic and foreign commercial vessels and cargo identification. Safety Enforcement activities included cargo safety, tracking, identification of hazardous materials, waterway safety regulations, and hazardous materials regulations. Homeland Security and Law Enforcement Applications included Radiological Dispersive Devices (RDD) identification, identification of unsafe or illicit transport of hazardous materials including chemicals and radiological materials, and screening for shipments of illicit drugs. In the Fall of 2005 the SensorNet funding for the project expired. After several unsuccessful attempts to find a Federal sponsor to continue with the project, the Watts Bar Dam Project was canceled and the Exploranium radiation monitors were removed from the doors of Watts Bar Dam in early 2006. The DHS Domestic Nuclear Detection Office decided to proceed with a Pilot building on the ORNL work performed at the TN and SC weigh stations in the highway sector of the Trusted Corridors project and eventually expanded it to other southern states under the name of Southeastern Corridor Pilot Project (SETCP). Many of the Phase I goals were achieved however real-world test data of private watercraft and barges was never obtained.« less

Detection of hidden explosives in different scenarios with the use of nuclear probes

NASA Astrophysics Data System (ADS)

Nebbia, G.; Pesente, S.; Lunardon, M.; Moretto, S.; Viesti, G.; Cinausero, M.; Barbui, M.; Fioretto, E.; Filippini, V.; Sudac, D.; Nađ, K.; Blagus, S.; Valković, V.

2005-04-01

The detection of landmines by using available technologies is a time consuming, expensive and extremely dangerous job, so that there is a need for a technological breakthrough in this field. Atomic and nuclear physics based sensors might offer new possibilities in de-mining. Technology and methods derived from the studies applied to the detection of landmines can be successfully applied to the screening of cargo in customs inspections.

Experimental demonstration of multiple monoenergetic gamma radiography for effective atomic number identification in cargo inspection

NASA Astrophysics Data System (ADS)

Henderson, Brian S.; Lee, Hin Y.; MacDonald, Thomas D.; Nelson, Roberts G.; Danagoulian, Areg

2018-04-01

The smuggling of special nuclear materials (SNMs) through international borders could enable nuclear terrorism and constitutes a significant threat to global security. This paper presents the experimental demonstration of a novel radiographic technique for quantitatively reconstructing the density and type of material present in commercial cargo containers, as a means of detecting such threats. Unlike traditional techniques which use sources of bremsstrahlung photons with a continuous distribution of energies, multiple monoenergetic gamma radiography utilizes monoenergetic photons from nuclear reactions, specifically the 4.4 and 15.1 MeV photons from the 11B(d,nγ)12C reaction. By exploiting the Z-dependence of the photon interaction cross sections at these two specific energies, it is possible to simultaneously determine the areal density and the effective atomic number as a function of location for a 2D projection of a scanned object. The additional information gleaned from using and detecting photons of specific energies for radiography substantially increases the resolving power between different materials. This paper presents results from the imaging of mock cargo materials ranging from Z ≈5 -92 , demonstrating accurate reconstruction of the effective atomic number and areal density of the materials over the full range. In particular, the system is capable of distinguishing pure materials with Z ≳ 70 , such as lead and uranium—a critical requirement of a system designed to detect SNM. This methodology could be used to screen commercial cargoes with high material specificity, to distinguish most benign materials from SNM, such as uranium and plutonium.

Combining Radiography and Passive Measurements for Radiological Threat Localization in Cargo

NASA Astrophysics Data System (ADS)

Miller, Erin A.; White, Timothy A.; Jarman, Kenneth D.; Kouzes, Richard T.; Kulisek, Jonathan A.; Robinson, Sean M.; Wittman, Richard A.

2015-10-01

Detecting shielded special nuclear material (SNM) in a cargo container is a difficult problem, since shielding reduces the amount of radiation escaping the container. Radiography provides information that is complementary to that provided by passive gamma-ray detection systems: while not directly sensitive to radiological materials, radiography can reveal highly shielded regions that may mask a passive radiological signal. Combining these measurements has the potential to improve SNM detection, either through improved sensitivity or by providing a solution to the inverse problem to estimate source properties (strength and location). We present a data-fusion method that uses a radiograph to provide an estimate of the radiation-transport environment for gamma rays from potential sources. This approach makes quantitative use of radiographic images without relying on image interpretation, and results in a probabilistic description of likely source locations and strengths. We present results for this method for a modeled test case of a cargo container passing through a plastic-scintillator-based radiation portal monitor and a transmission-radiography system. We find that a radiograph-based inversion scheme allows for localization of a low-noise source placed randomly within the test container to within 40 cm, compared to 70 cm for triangulation alone, while strength estimation accuracy is improved by a factor of six. Improvements are seen in regions of both high and low shielding, but are most pronounced in highly shielded regions. The approach proposed here combines transmission and emission data in a manner that has not been explored in the cargo-screening literature, advancing the ability to accurately describe a hidden source based on currently-available instrumentation.

Active transport on disordered microtubule networks: the generalized random velocity model.

PubMed

Kahana, Aviv; Kenan, Gilad; Feingold, Mario; Elbaum, Michael; Granek, Rony

2008-11-01

The motion of small cargo particles on microtubules by means of motor proteins in disordered microtubule networks is investigated theoretically using both analytical tools and computer simulations. Different network topologies in two and three dimensions are considered, one of which has been recently studied experimentally by Salman [Biophys. J. 89, 2134 (2005)]. A generalization of the random velocity model is used to derive the mean-square displacement of the cargo particle. We find that all cases belong to the class of anomalous superdiffusion, which is sensitive mainly to the dimensionality of the network and only marginally to its topology. Yet in three dimensions the motion is very close to simple diffusion, with sublogarithmic corrections that depend on the network topology. When details of the thermal diffusion in the bulk solution are included, no significant change to the asymptotic time behavior is found. However, a small asymmetry in the mean microtubule polarity affects the corresponding long-time behavior. We also study a three-dimensional model of the microtubule network in living animal cells. Three first-passage-time problems of intracellular transport are simulated and analyzed for different motor processivities: (i) cargo that originates near the nucleus and has to reach the membrane, (ii) cargo that originates from the membrane and has to reach the nucleus, and (iii) cargo that leaves the nucleus and has to reach a specific target in the cytoplasm. We conclude that while a higher motor processivity increases the transport efficiency in cases (i) and (ii), in case (iii) it has the opposite effect. We conjecture that the balance between the different network tasks, as manifested in cases (i) and (ii) versus case (iii), may be the reason for the evolutionary choice of a finite motor processivity.

Active transport on disordered microtubule networks: The generalized random velocity model

NASA Astrophysics Data System (ADS)

Kahana, Aviv; Kenan, Gilad; Feingold, Mario; Elbaum, Michael; Granek, Rony

2008-11-01

The motion of small cargo particles on microtubules by means of motor proteins in disordered microtubule networks is investigated theoretically using both analytical tools and computer simulations. Different network topologies in two and three dimensions are considered, one of which has been recently studied experimentally by Salman [Biophys. J. 89, 2134 (2005)]. A generalization of the random velocity model is used to derive the mean-square displacement of the cargo particle. We find that all cases belong to the class of anomalous superdiffusion, which is sensitive mainly to the dimensionality of the network and only marginally to its topology. Yet in three dimensions the motion is very close to simple diffusion, with sublogarithmic corrections that depend on the network topology. When details of the thermal diffusion in the bulk solution are included, no significant change to the asymptotic time behavior is found. However, a small asymmetry in the mean microtubule polarity affects the corresponding long-time behavior. We also study a three-dimensional model of the microtubule network in living animal cells. Three first-passage-time problems of intracellular transport are simulated and analyzed for different motor processivities: (i) cargo that originates near the nucleus and has to reach the membrane, (ii) cargo that originates from the membrane and has to reach the nucleus, and (iii) cargo that leaves the nucleus and has to reach a specific target in the cytoplasm. We conclude that while a higher motor processivity increases the transport efficiency in cases (i) and (ii), in case (iii) it has the opposite effect. We conjecture that the balance between the different network tasks, as manifested in cases (i) and (ii) versus case (iii), may be the reason for the evolutionary choice of a finite motor processivity.

Straddle carrier radiation portal monitoring

NASA Astrophysics Data System (ADS)

Andersen, Eric S.; Samuel, Todd J.; Mullen, O. Dennis

2005-05-01

U.S. Customs and Border Protection (CBP) is the primary enforcement agency protecting the nation"s ports of entry. CBP is enhancing its capability to interdict the illicit import of nuclear and radiological materials and devices that may be used by terrorists. Pacific Northwest National Laboratory (PNNL) is providing scientific and technical support to CBP in their goal to enable rapid deployment of nuclear and radiation detection systems at U. S. ports of entry to monitor 100% of the incoming international traffic and cargo while not adversely impacting the operations or throughput of the ports. The U.S. ports of entry include the following vectors: land border crossings, seaports, airports, rail crossings, and mail and express consignment courier facilities. U.S. Customs and Border Protection (CBP) determined that a screening solution was needed for Seaport cargo containers being transported by Straddle Carriers (straddle carriers). A stationary Radiation Portal Monitor (RPM) for Straddle Carriers (SCRPM) is needed so that cargo containers can be scanned while in transit under a Straddle Carrier. The Straddle Carrier Portal operational impacts were minimized by conducting a time-motion study at the Port, and adaptation of a Remotely Operated RPM (RO-RPM) booth concept that uses logical lighting schemes for traffic control, cameras, Optical Character Recognition, and wireless technology.

Straddle Carrier Radiation Portal Monitoring

DOE Office of Scientific and Technical Information (OSTI.GOV)

Andersen, Eric S.; Samuel, Todd J.; Mullen, O Dennis

2005-08-01

U.S. Customs and Border Protection (CBP) is the primary enforcement agency protecting the nation’s ports of entry. CBP is enhancing its capability to interdict the illicit import of nuclear and radiological materials and devices that may be used by terrorists. Pacific Northwest National Laboratory (PNNL) is providing scientific and technical support to CBP in their goal to enable rapid deployment of nuclear and radiation detection systems at U. S. ports of entry to monitor 100% of the incoming international traffic and cargo while not adversely impacting the operations or throughput of the ports. The U.S. ports of entry include themore » following vectors: land border crossings, seaports, airports, rail crossings, and mail and express consignment courier facilities. U.S. Customs and Border Protection (CBP) determined that a screening solution was needed for Seaport cargo containers being transported by Straddle Carriers (straddle carriers). A stationary Radiation Portal Monitor (RPM) for Straddle Carriers (SCRPM) is needed so that cargo containers can be scanned while in transit under a Straddle Carrier. The Straddle Carrier Portal operational impacts were minimized by conducting a time-motion study at the Port, and adaptation of a Remotely Operated RPM (RO-RPM) booth concept that uses logical lighting schemes for traffic control, cameras, Optical Character Recognition, and wireless technology.« less

A binned clustering algorithm to detect high-Z material using cosmic muons

NASA Astrophysics Data System (ADS)

Thomay, C.; Velthuis, J. J.; Baesso, P.; Cussans, D.; Morris, P. A. W.; Steer, C.; Burns, J.; Quillin, S.; Stapleton, M.

2013-10-01

We present a novel approach to the detection of special nuclear material using cosmic rays. Muon Scattering Tomography (MST) is a method for using cosmic muons to scan cargo containers and vehicles for special nuclear material. Cosmic muons are abundant, highly penetrating, not harmful for organic tissue, cannot be screened against, and can easily be detected, which makes them highly suited to the use of cargo scanning. Muons undergo multiple Coulomb scattering when passing through material, and the amount of scattering is roughly proportional to the square of the atomic number Z of the material. By reconstructing incoming and outgoing tracks, we can obtain variables to identify high-Z material. In a real life application, this has to happen on a timescale of 1 min and thus with small numbers of muons. We have built a detector system using resistive plate chambers (RPCs): 12 layers of RPCs allow for the readout of 6 x and 6 y positions, by which we can reconstruct incoming and outgoing tracks. In this work we detail the performance of an algorithm by which we separate high-Z targets from low-Z background, both for real data from our prototype setup and for MC simulation of a cargo container-sized setup. (c) British Crown Owned Copyright 2013/AWE

MPLM Donatello is offloaded at the SLF

NASA Technical Reports Server (NTRS)

2001-01-01

At the Shuttle Landing Facility, workers in cherry pickers (right) help guide offloading of the Italian Space Agency's Multi-Purpose Logistics Module Donatello from the Airbus '''Beluga''' air cargo plane that brought it from the factory of Alenia Aerospazio in Turin, Italy. The third of three for the International Space Station, the module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

Modernization of dump truck onboard system

NASA Astrophysics Data System (ADS)

Semenov, M. A.; Bolshunova, O. M.; Korzhev, A. A.; Kamyshyan, A. M.

2017-10-01

The review of the only automated dispatch system for the career dump trucks, which is presented in the domestic market, was made. A method for upgrading the loading control system and technological weighing process of the career dump was proposed. The cargo weight during loading is determined by the gas pressure in the suspension cylinders at the time of the oscillation ending and at the start of the vibration smoothing process; the smoothing speed correction is performed. The error of the cargo weighting is 2.5-3%, and of the technological weighing process during driving - 1%, which corresponds to the error level of the steady-state weighting means.

An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor

PubMed Central

Dagdas, Yasin F; Belhaj, Khaoula; Maqbool, Abbas; Chaparro-Garcia, Angela; Pandey, Pooja; Petre, Benjamin; Tabassum, Nadra; Cruz-Mireles, Neftaly; Hughes, Richard K; Sklenar, Jan; Win, Joe; Menke, Frank; Findlay, Kim; Banfield, Mark J; Kamoun, Sophien; Bozkurt, Tolga O

2016-01-01

Plants use autophagy to safeguard against infectious diseases. However, how plant pathogens interfere with autophagy-related processes is unknown. Here, we show that PexRD54, an effector from the Irish potato famine pathogen Phytophthora infestans, binds host autophagy protein ATG8CL to stimulate autophagosome formation. PexRD54 depletes the autophagy cargo receptor Joka2 out of ATG8CL complexes and interferes with Joka2's positive effect on pathogen defense. Thus, a plant pathogen effector has evolved to antagonize a host autophagy cargo receptor to counteract host defenses. DOI: http://dx.doi.org/10.7554/eLife.10856.001 PMID:26765567

Explosives Detection: Exploitation of the Physical Signatures

NASA Astrophysics Data System (ADS)

Atkinson, David

2010-10-01

Explosives based terrorism is an ongoing threat that is evolving with respect to implementation, configuration and materials used. There are a variety of devices designed to detect explosive devices, however, each technology has limitations and operational constraints. A full understanding of the signatures available for detection coupled with the array of detection choices can be used to develop a conceptual model of an explosives screening operation. Physics based sensors provide a robust approach to explosives detection, typically through the identification of anomalies, and are currently used for screening in airports around the world. The next generation of detectors for explosives detection will need to be more sensitive and selective, as well as integrate seamlessly with devices focused on chemical signatures. An appreciation for the details of the physical signature exploitation in cluttered environments with time, space, and privacy constraints is necessary for effective explosives screening of people, luggage, cargo, and vehicles.

Capacity utilization study for aviation security cargo inspection queuing system

NASA Astrophysics Data System (ADS)

Allgood, Glenn O.; Olama, Mohammed M.; Lake, Joe E.; Brumback, Daryl

2010-04-01

In this paper, we conduct performance evaluation study for an aviation security cargo inspection queuing system for material flow and accountability. The queuing model employed in our study is based on discrete-event simulation and processes various types of cargo simultaneously. Onsite measurements are collected in an airport facility to validate the queuing model. The overall performance of the aviation security cargo inspection system is computed, analyzed, and optimized for the different system dynamics. Various performance measures are considered such as system capacity, residual capacity, throughput, capacity utilization, subscribed capacity utilization, resources capacity utilization, subscribed resources capacity utilization, and number of cargo pieces (or pallets) in the different queues. These metrics are performance indicators of the system's ability to service current needs and response capacity to additional requests. We studied and analyzed different scenarios by changing various model parameters such as number of pieces per pallet, number of TSA inspectors and ATS personnel, number of forklifts, number of explosives trace detection (ETD) and explosives detection system (EDS) inspection machines, inspection modality distribution, alarm rate, and cargo closeout time. The increased physical understanding resulting from execution of the queuing model utilizing these vetted performance measures should reduce the overall cost and shipping delays associated with new inspection requirements.

Early and Selective Impairments in Axonal Transport Kinetics of Synaptic Cargoes Induced by Soluble Amyloid β-Protein Oligomers

PubMed Central

Tang, Yong; Scott, David A.; Das, Utpal; Edland, Steven D.; Radomski, Kryslaine; Koo, Edward H.; Roy, Subhojit

2013-01-01

The downstream targets of amyloid β (Aβ)-oligomers remain elusive. One hypothesis is that Aβ-oligomers interrupt axonal transport. Although previous studies have demonstrated Aβ-induced transport blockade, early effects of low-n soluble Aβ-oligomers on axonal transport remain unclear. Furthermore, the cargo selectivity for such deficits (if any) or the specific effects of Aβ on the motility kinetics of transported cargoes are also unknown. Toward this, we visualized axonal transport of vesicles in cultured hippocampal neurons treated with picomolar (pm) levels of cell-derived soluble Aβ-oligomers. We examined select cargoes thought to move as distinct organelles and established imaging parameters that allow organelle tracking with consistency and high fidelity – analyzing all data in a blinded fashion. Aβ-oligomers induced early and selective diminutions in velocities of synaptic cargoes but had no effect on mitochondrial motility, contrary to previous reports. These changes were N-methyl d-aspartate receptor/glycogen synthase kinase-3β dependent and reversible upon washout of the oligomers. Cluster-mode analyses reveal selective attenuations in faster-moving synaptic vesicles, suggesting possible decreases in cargo/motor associations, and biochemical experiments implicate tau phosphorylation in the process. Collectively, the data provide a biological basis for Aβ-induced axonal transport deficits. PMID:22309053

Capacity Utilization Study for Aviation Security Cargo Inspection Queuing System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Allgood, Glenn O; Olama, Mohammed M; Lake, Joe E

In this paper, we conduct performance evaluation study for an aviation security cargo inspection queuing system for material flow and accountability. The queuing model employed in our study is based on discrete-event simulation and processes various types of cargo simultaneously. Onsite measurements are collected in an airport facility to validate the queuing model. The overall performance of the aviation security cargo inspection system is computed, analyzed, and optimized for the different system dynamics. Various performance measures are considered such as system capacity, residual capacity, throughput, capacity utilization, subscribed capacity utilization, resources capacity utilization, subscribed resources capacity utilization, and number ofmore » cargo pieces (or pallets) in the different queues. These metrics are performance indicators of the system s ability to service current needs and response capacity to additional requests. We studied and analyzed different scenarios by changing various model parameters such as number of pieces per pallet, number of TSA inspectors and ATS personnel, number of forklifts, number of explosives trace detection (ETD) and explosives detection system (EDS) inspection machines, inspection modality distribution, alarm rate, and cargo closeout time. The increased physical understanding resulting from execution of the queuing model utilizing these vetted performance measures should reduce the overall cost and shipping delays associated with new inspection requirements.« less

ICRP Publication 125: Radiological Protection in Security Screening.

PubMed

Cool, D A; Lazo, E; Tattersall, P; Simeonov, G; Niu, S

2014-07-01

The use of technologies to provide security screening for individuals and objects has been increasing rapidly, in keeping with the significant increase in security concerns worldwide. Within the spectrum of technologies, the use of ionizing radiation to provide backscatter and transmission screening capabilities has also increased. The Commission has previously made a number of statements related to the general topic of deliberate exposures of individuals in non-medical settings. This report provides advice on how the radiological protection principles recommended by the Commission should be applied within the context of security screening. More specifically, the principles of justification, optimisation of protection, and dose limitation for planned exposure situations are directly applicable to the use of ionising radiation in security screening. In addition, several specific topics are considered in this report, including the situation in which individuals may be exposed because they are concealed (‘stowaways’) in a cargo container or conveyance that may be subject to screening. The Commission continues to recommend that careful justification of screening should be considered before decisions are made to employ the technology. If a decision is made that its use is justified, the framework for protection as a planned exposure situation should be employed, including optimization of protection with the use of dose constraints and the appropriate provisions for authorisation and inspection.

A whole-system approach to x-ray spectroscopy in cargo inspection systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Langeveld, Willem G. J.; Gozani, Tsahi; Ryge, Peter

The bremsstrahlung x-ray spectrum used in high-energy, high-intensity x-ray cargo inspection systems is attenuated and modified by the materials in the cargo in a Z-dependent way. Therefore, spectroscopy of the detected x rays yields information about the Z of the x-rayed cargo material. It has previously been shown that such ZSpectroscopy (Z-SPEC) is possible under certain circumstances. A statistical approach, Z-SCAN (Z-determination by Statistical Count-rate ANalysis), has also been shown to be effective, and it can be used either by itself or in conjunction with Z-SPEC when the x-ray count rate is too high for individual x-ray spectroscopy. Both techniquesmore » require fast x-ray detectors and fast digitization electronics. It is desirable (and possible) to combine all techniques, including x-ray imaging of the cargo, in a single detector array, to reduce costs, weight, and overall complexity. In this paper, we take a whole-system approach to x-ray spectroscopy in x-ray cargo inspection systems, and show how the various parts interact with one another. Faster detectors and read-out electronics are beneficial for both techniques. A higher duty-factor x-ray source allows lower instantaneous count rates at the same overall x-ray intensity, improving the range of applicability of Z-SPEC in particular. Using an intensity-modulated advanced x-ray source (IMAXS) allows reducing the x-ray count rate for cargoes with higher transmission, and a stacked-detector approach may help material discrimination for the lowest attenuations. Image processing and segmentation allow derivation of results for entire objects, and subtraction of backgrounds. We discuss R and D performed under a number of different programs, showing progress made in each of the interacting subsystems. We discuss results of studies into faster scintillation detectors, including ZnO, BaF{sub 2} and PbWO{sub 4}, as well as suitable photo-detectors, read-out and digitization electronics. We discuss high-duty-factor linear-accelerator x-ray sources and their associated requirements, and how such sources improve spectroscopic techniques. We further discuss how image processing techniques help in correcting for backgrounds and overlapping materials. In sum, we present an integrated picture of how to optimize a cargo inspection system for x-ray spectroscopy.« less

Efficient material decomposition method for dual-energy X-ray cargo inspection system

NASA Astrophysics Data System (ADS)

Lee, Donghyeon; Lee, Jiseoc; Min, Jonghwan; Lee, Byungcheol; Lee, Byeongno; Oh, Kyungmin; Kim, Jaehyun; Cho, Seungryong

2018-03-01

Dual-energy X-ray inspection systems are widely used today for it provides X-ray attenuation contrast of the imaged object and also its material information. Material decomposition capability allows a higher detection sensitivity of potential targets including purposely loaded impurities in agricultural product inspections and threats in security scans for example. Dual-energy X-ray transmission data can be transformed into two basis material thickness data, and its transformation accuracy heavily relies on a calibration of material decomposition process. The calibration process in general can be laborious and time consuming. Moreover, a conventional calibration method is often challenged by the nonuniform spectral characteristics of the X-ray beam in the entire field-of-view (FOV). In this work, we developed an efficient material decomposition calibration process for a linear accelerator (LINAC) based high-energy X-ray cargo inspection system. We also proposed a multi-spot calibration method to improve the decomposition performance throughout the entire FOV. Experimental validation of the proposed method has been demonstrated by use of a cargo inspection system that supports 6 MV and 9 MV dual-energy imaging.

Combining Radiography and Passive Measurements for Radiological Threat Localization in Cargo

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, Erin A.; White, Timothy A.; Jarman, Kenneth D.

Detecting shielded special nuclear material (SNM) in a cargo container is a difficult problem, since shielding reduces the amount of radiation escaping the container. Radiography provides information that is complementary to that provided by passive gamma-ray detection systems: while not directly sensitive to radiological materials, radiography can reveal highly shielded regions that may mask a passive radiological signal. Combining these measurements has the potential to improve SNM detection, either through improved sensitivity or by providing a solution to the inverse problem to estimate source properties (strength and location). We present a data-fusion method that uses a radiograph to provide anmore » estimate of the radiation-transport environment for gamma rays from potential sources. This approach makes quantitative use of radiographic images without relying on image interpretation, and results in a probabilistic description of likely source locations and strengths. We present results for this method for a modeled test case of a cargo container passing through a plastic-scintillator-based radiation portal monitor and a transmission-radiography system. We find that a radiograph-based inversion scheme allows for localization of a low-noise source placed randomly within the test container to within 40 cm, compared to 70 cm for triangulation alone, while strength estimation accuracy is improved by a factor of six. Improvements are seen in regions of both high and low shielding, but are most pronounced in highly shielded regions. The approach proposed here combines transmission and emission data in a manner that has not been explored in the cargo-screening literature, advancing the ability to accurately describe a hidden source based on currently-available instrumentation.« less

Power Supply for a Manned International Asteroid Mission

NASA Technical Reports Server (NTRS)

Weingartner, Stefan; Nahra, Henry K.; Kohout, Lisa L.; Larin, Max

1991-01-01

A feasibility study considering the exploitation of a near Earth asteroid was performed. The power requirements and proposed power systems for the crew vehicle, cargo vehicles, mining and processing equipment are described. A photovoltaic power system was selected to meet the 52.1 kWe and the 3.9 kWe power requirements of the crew and cargo vehicles, respectively. A nuclear power plant using a thermodynamic Rankine cycle with a total mass of 62.1 tons was chosen to provide the 7.225 MWe and the 5.5 MWth required for the mining and processing activities at the asteroid.

Airlift Cargo Hub Port Hold Times: Controlling Variations in Defense Supply Chain Delivery

DTIC Science & Technology

2010-06-01

AIR UNIVERSITY AIR FORCE INSTITUTE OF TECHNOLOGY Wright-Patterson Air Force Base, Ohio APPROVED FOR PUBLIC RELEASE; DISTRIBUTION UNLIMITED...Department of Operational Sciences Graduate School of Engineering and Management Air Force Institute of Technology Air University Air...process for a seamless flow of cargo onto the aircraft segment (AMC and DDC , 24 Sep 08). Figure 6 AMC Velocity Efforts (Anderson D. , 2009) While

Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles

PubMed Central

Lukianova-Hleb, Ekaterina Y.; Wagner, Daniel S.; Brenner, Malcolm K.; Lapotko, Dmitri O.

2012-01-01

Optimal cell therapies require efficient, selective and rapid delivery of molecular cargo into target cells without compromising their viability. Achieving these goals ex vivo in bulk heterogeneous multi-cell systems such as human grafts is impeded by low selectivity and speed of cargo delivery and by significant damage to target and non-target cells. We have developed a cell level approach for selective and guided trans-membrane injection of extracellular cargo into specific target cells using transient plasmonic nanobubbles (PNB) as cell-specific nano-injectors. As a technical platform for this method we developed a laser flow cell processing system. The PNB injection method and flow system were tested in heterogeneous cell suspensions of target and non-target cells for delivery of Dextran-FITC dye into squamous cell carcinoma HN31 cells and transfection of human T-cells with a green fluorescent protein-encoding plasmid. In both models the method demonstrated single cell type selectivity, high efficacy of delivery (96% both for HN31 cells T-cells), speed of delivery (nanoseconds) and viability of treated target cells (96% for HN31 cells and 75% for T-cells). The PNB injection method may therefore be beneficial for real time processing of human grafts without removal of physiologically important cells. PMID:22521612

Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles.

PubMed

Lukianova-Hleb, Ekaterina Y; Wagner, Daniel S; Brenner, Malcolm K; Lapotko, Dmitri O

2012-07-01

Optimal cell therapies require efficient, selective and rapid delivery of molecular cargo into target cells without compromising their viability. Achieving these goals ex vivo in bulk heterogeneous multi-cell systems such as human grafts is impeded by low selectivity and speed of cargo delivery and by significant damage to target and non-target cells. We have developed a cell level approach for selective and guided transmembrane injection of extracellular cargo into specific target cells using transient plasmonic nanobubbles (PNB) as cell-specific nano-injectors. As a technical platform for this method we developed a laser flow cell processing system. The PNB injection method and flow system were tested in heterogeneous cell suspensions of target and non-target cells for delivery of Dextran-FITC dye into squamous cell carcinoma HN31 cells and transfection of human T-cells with a green fluorescent protein-encoding plasmid. In both models the method demonstrated single cell type selectivity, high efficacy of delivery (96% both for HN31 cells T-cells), speed of delivery (nanoseconds) and viability of treated target cells (96% for HN31 cells and 75% for T-cells). The PNB injection method may therefore be beneficial for real time processing of human grafts without removal of physiologically important cells. Copyright © 2012 Elsevier Ltd. All rights reserved.

Bi-stability in cooperative transport by ants in the presence of obstacles

PubMed Central

Pinkoviezky, Itai; Feinerman, Ofer

2018-01-01

To cooperatively carry large food items to the nest, individual ants conform their efforts and coordinate their motion. Throughout this expedition, collective motion is driven both by internal interactions between the carrying ants and a response to newly arrived informed ants that orient the cargo towards the nest. During the transport process, the carrying group must overcome obstacles that block their path to the nest. Here, we investigate the dynamics of cooperative transport, when the motion of the ants is frustrated by a linear obstacle that obstructs the motion of the cargo. The obstacle contains a narrow opening that serves as the only available passage to the nest, and through which single ants can pass but not with the cargo. We provide an analytical model for the ant-cargo system in the constrained environment that predicts a bi-stable dynamic behavior between an oscillatory mode of motion along the obstacle and a convergent mode of motion near the opening. Using both experiments and simulations, we show how for small cargo sizes, the system exhibits spontaneous transitions between these two modes of motion due to fluctuations in the applied force on the cargo. The bi-stability provides two possible problem solving strategies for overcoming the obstacle, either by attempting to pass through the opening, or take large excursions to circumvent the obstacle. PMID:29746457

Bi-stability in cooperative transport by ants in the presence of obstacles.

PubMed

Ron, Jonathan E; Pinkoviezky, Itai; Fonio, Ehud; Feinerman, Ofer; Gov, Nir S

2018-05-01

To cooperatively carry large food items to the nest, individual ants conform their efforts and coordinate their motion. Throughout this expedition, collective motion is driven both by internal interactions between the carrying ants and a response to newly arrived informed ants that orient the cargo towards the nest. During the transport process, the carrying group must overcome obstacles that block their path to the nest. Here, we investigate the dynamics of cooperative transport, when the motion of the ants is frustrated by a linear obstacle that obstructs the motion of the cargo. The obstacle contains a narrow opening that serves as the only available passage to the nest, and through which single ants can pass but not with the cargo. We provide an analytical model for the ant-cargo system in the constrained environment that predicts a bi-stable dynamic behavior between an oscillatory mode of motion along the obstacle and a convergent mode of motion near the opening. Using both experiments and simulations, we show how for small cargo sizes, the system exhibits spontaneous transitions between these two modes of motion due to fluctuations in the applied force on the cargo. The bi-stability provides two possible problem solving strategies for overcoming the obstacle, either by attempting to pass through the opening, or take large excursions to circumvent the obstacle.

Security inspection in ports by anomaly detection using hyperspectral imaging technology

NASA Astrophysics Data System (ADS)

Rivera, Javier; Valverde, Fernando; Saldaña, Manuel; Manian, Vidya

2013-05-01

Applying hyperspectral imaging technology in port security is crucial for the detection of possible threats or illegal activities. One of the most common problems that cargo suffers is tampering. This represents a danger to society because it creates a channel to smuggle illegal and hazardous products. If a cargo is altered, security inspections on that cargo should contain anomalies that reveal the nature of the tampering. Hyperspectral images can detect anomalies by gathering information through multiple electromagnetic bands. The spectrums extracted from these bands can be used to detect surface anomalies from different materials. Based on this technology, a scenario was built in which a hyperspectral camera was used to inspect the cargo for any surface anomalies and a user interface shows the results. The spectrum of items, altered by different materials that can be used to conceal illegal products, is analyzed and classified in order to provide information about the tampered cargo. The image is analyzed with a variety of techniques such as multiple features extracting algorithms, autonomous anomaly detection, and target spectrum detection. The results will be exported to a workstation or mobile device in order to show them in an easy -to-use interface. This process could enhance the current capabilities of security systems that are already implemented, providing a more complete approach to detect threats and illegal cargo.

Polymer-Based Nanofibers Impregnated with Drug Infused Plant Virus Particles as a Responsive Fabric for Therapeutic Delivery

NASA Astrophysics Data System (ADS)

Honarbakhsh, Sara

A biodegradable and controlled drug delivery system has been developed herein composed of electrospun polymeric nanofibers impregnated with cargo loaded Red clover necrotic mosaic virus (RCNMV)---a robust plant virus---as the drug carrier nanoparticle. In this system, controlled drug release is achieved by altering the porosity of the biodegradable matrix as well as controlling the position and distribution of the cargo loaded nanocarriers in the matrix. Solution electrospinning as well as dipping method are used to create and to impregnate the matrix (the fibers of which possess uniformly distributed nano-size surface pores) with cargo loaded nanocarriers. Prior to the impregnation stage of cargo loaded nanocarriers into the matrix, compatibility of a group of candidate cargos (Ampicillin, Novanthrone, Doxorubicin and Ethidium Bromide) and RCNMV functionality with potential electrospinning solvents were investigated and a solvent with the least degradative effect was selected. In order to achieve both sustained and immediate drug release profiles, cargo loaded nanocarriers were embedded into the matrix---through co-spinning process---as well as on the surface of matrix fibers---through dipping method. SEM, TEM and Fluorescent Light Microscopy images of the medicated structures suggested that the nanocarriers were incorporated into/on the matrix. In vitro release assays were also carried out the results of which confirmed having obtained sustained release in the co-spun medicated structures where as dipped samples showed an immediate release profile.

STS-116 Flight Controllers on console during mission - WFCR - Orbit 2

NASA Image and Video Library

2006-12-20

JSC2006-E-54711 (21 Dec. 2006) --- Overall view of the Shuttle Flight Control Room in the Johnson Space Center's Mission Control Center during the final deployment of some small satellites from Space Shuttle Discovery's cargo bay. On a screen in the front of the control room, a Department of Defense pico-satellite known as Atmospheric Neutral Density Experiment (ANDE) is released from the shuttle's payload bay by STS-116 crewmembers and viewed via live television on the ground.

Cargo contraband screening

NASA Astrophysics Data System (ADS)

Kim, L. H.; Danylewich-May, Lucy L.; Jadamec, Joseph R.; Su, Chih-Wu; Rigdon, Stephen W.; Norwood, LaVan J.; Hoglund, David E.

1994-10-01

The IONSCANTM operating parameters for detection of benzoic acid and acetic acid have been optimized. Various materials and chromatographic coatings were investigated for the vapor preconcentration of benzoic acid, acetic acid and cocaine. Limited field trials indicated a possibility of detecting benzoic acid from seized cocaine shipment. From the laboratory investigation and some field tests it has been shown that cocaine free base is present in cocaine hydrochloride in sufficiently high concentration to provide constant supply of cocaine vapors in the headspace for a long period of time.

Nondestructive inspection of explosive materials using linearly polarized two-colored photon beam

NASA Astrophysics Data System (ADS)

Toyokawa, H.; Hayakawa, T.; Shizuma, T.; Hajima, R.; Masuda, K.; Ohgaki, H.

2011-10-01

A nondestructive inspection method for screening explosive materials that are hidden in passenger vehicles, trucks, and cargo containers with radiation shielding was presented. The method was examined experimentally using linearly polarized two-colored photon beam. A sample object was irradiated with the photon beam, followed by an emission of gamma-rays in nuclear resonance fluorescence. The gamma-rays from oxygen and nitrogen emitted through nuclear resonance fluorescence were measured using high-purity germanium detectors. We were able to evaluate the element concentration ratio.

Cargo crowding at actin-rich regions along axons causes local traffic jams.

PubMed

Sood, Parul; Murthy, Kausalya; Kumar, Vinod; Nonet, Michael L; Menon, Gautam I; Koushika, Sandhya P

2018-03-01

Steady axonal cargo flow is central to the functioning of healthy neurons. However, a substantial fraction of cargo in axons remains stationary up to several minutes. We examine the transport of precursors of synaptic vesicles (pre-SVs), endosomes and mitochondria in Caenorhabditis elegans touch receptor neurons, showing that stationary cargo are predominantly present at actin-rich regions along the neuronal process. Stationary vesicles at actin-rich regions increase the propensity of moving vesicles to stall at the same location, resulting in traffic jams arising from physical crowding. Such local traffic jams at actin-rich regions are likely to be a general feature of axonal transport since they also occur in Drosophila neurons. Repeated touch stimulation of C. elegans reduces the density of stationary pre-SVs, indicating that these traffic jams can act as both sources and sinks of vesicles. This suggests that vesicles trapped in actin-rich regions are functional reservoirs that may contribute to maintaining robust cargo flow in the neuron. A video abstract of this article can be found at: Video S1; Video S2. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Monitoring Extracellular Vesicle Cargo Active Uptake by Imaging Flow Cytometry.

PubMed

Ofir-Birin, Yifat; Abou Karam, Paula; Rudik, Ariel; Giladi, Tal; Porat, Ziv; Regev-Rudzki, Neta

2018-01-01

Extracellular vesicles are essential for long distance cell-cell communication. They function as carriers of different compounds, including proteins, lipids and nucleic acids. Pathogens, like malaria parasites ( Plasmodium falciparum, Pf ), excel in employing vesicle release to mediate cell communication in diverse processes, particularly in manipulating the host response. Establishing research tools to study the interface between pathogen-derived vesicles and their host recipient cells will greatly benefit the scientific community. Here, we present an imaging flow cytometry (IFC) method for monitoring the uptake of malaria-derived vesicles by host immune cells. By staining different cargo components, we were able to directly track the cargo's internalization over time and measure the kinetics of its delivery. Impressively, we demonstrate that this method can be used to specifically monitor the translocation of a specific protein within the cellular milieu upon internalization of parasitic cargo; namely, we were able to visually observe how uptaken parasitic Pf -DNA cargo leads to translocation of transcription factor IRF3 from the cytosol to the nucleus within the recipient immune cell. Our findings demonstrate that our method can be used to study cellular dynamics upon vesicle uptake in different host-pathogen and pathogen-pathogen systems.

MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network.

PubMed

Sauer, Michael; Delgadillo, M Otilia; Zouhar, Jan; Reynolds, Gregory D; Pennington, Janice G; Jiang, Liwen; Liljegren, Sarah J; Stierhof, York-Dieter; De Jaeger, Geert; Otegui, Marisa S; Bednarek, Sebastian Y; Rojo, Enrique

2013-06-01

Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. In particular, it is unknown whether clathrin-coated vesicles (CCVs) participate in this transport step. Through a screen for modified transport to the vacuole (mtv) mutants that secrete the vacuolar protein VAC2, we identified MTV1, which encodes an epsin N-terminal homology protein, and MTV4, which encodes the ADP ribosylation factor GTPase-activating protein nevershed/AGD5. MTV1 and NEV/AGD5 have overlapping expression patterns and interact genetically to transport vacuolar cargo and promote plant growth, but they have no apparent roles in protein secretion or endocytosis. MTV1 and NEV/AGD5 colocalize with clathrin at the TGN and are incorporated into CCVs. Importantly, mtv1 nev/agd5 double mutants show altered subcellular distribution of CCV cargo exported from the TGN. Moreover, MTV1 binds clathrin in vitro, and NEV/AGD5 associates in vivo with clathrin, directly linking these proteins to CCV formation. These results indicate that MTV1 and NEV/AGD5 are key effectors for CCV-mediated trafficking of vacuolar proteins from the TGN to the PVC in plants.

Filling of a Poisson trap by a population of random intermittent searchers.

PubMed

Bressloff, Paul C; Newby, Jay M

2012-03-01

We extend the continuum theory of random intermittent search processes to the case of N independent searchers looking to deliver cargo to a single hidden target located somewhere on a semi-infinite track. Each searcher randomly switches between a stationary state and either a leftward or rightward constant velocity state. We assume that all of the particles start at one end of the track and realize sample trajectories independently generated from the same underlying stochastic process. The hidden target is treated as a partially absorbing trap in which a particle can only detect the target and deliver its cargo if it is stationary and within range of the target; the particle is removed from the system after delivering its cargo. As a further generalization of previous models, we assume that up to n successive particles can find the target and deliver its cargo. Assuming that the rate of target detection scales as 1/N, we show that there exists a well-defined mean-field limit N→∞, in which the stochastic model reduces to a deterministic system of linear reaction-hyperbolic equations for the concentrations of particles in each of the internal states. These equations decouple from the stochastic process associated with filling the target with cargo. The latter can be modeled as a Poisson process in which the time-dependent rate of filling λ(t) depends on the concentration of stationary particles within the target domain. Hence, we refer to the target as a Poisson trap. We analyze the efficiency of filling the Poisson trap with n particles in terms of the waiting time density f(n)(t). The latter is determined by the integrated Poisson rate μ(t)=∫(0)(t)λ(s)ds, which in turn depends on the solution to the reaction-hyperbolic equations. We obtain an approximate solution for the particle concentrations by reducing the system of reaction-hyperbolic equations to a scalar advection-diffusion equation using a quasisteady-state analysis. We compare our analytical results for the mean-field model with Monte Carlo simulations for finite N. We thus determine how the mean first passage time (MFPT) for filling the target depends on N and n.

Coarse-grained Simulations of Viral Assembly

NASA Astrophysics Data System (ADS)

Elrad, Oren M.

2011-12-01

The formation of viral capsids is a marvel of natural engineering and design. A large number (from 60 to thousands) of protein subunits assemble into complete, reproducible structures under a variety of conditions while avoiding kinetic and thermodynamic traps. Small single-stranded RNA viruses not only assemble their coat proteins in this fashion but also package their genome during the self-assembly process. Recent experiments have shown that the coat proteins are competent to assemble not merely around their own genomes but heterologous RNA, synthetic polyanions and even functionalized gold nanoparticles. Remarkably these viruses can even assemble around cargo not commensurate with their native state by adopting different morphologies. Understanding the properties that confer such exquisite precision and flexibility to the assembly process could aid biomedical research in the search for novel antiviral remedies, drug-delivery vehicles and contrast agents used in bioimaging. At the same time, viral assembly provides an excellent model system for the development of a statistical mechanical understanding of biological self-assembly, in the hopes of that we will identify some universal principles that underly such processes. This work consists of computational studies using coarse-grained representations of viral coat proteins and their cargoes. We find the relative strength of protein-cargo and protein-protein interactions has a profound effect on the assembly pathway, in some cases leading to assembly mechanisms that are markedly different from those found in previous work on the assembly of empty capsids. In the case of polymeric cargo, we find the first evidence for a previously theorized mechanism in which the polymer actively participates in recruiting free subunits to the assembly process through cooperative polymer-protein motions. We find that successful assembly is non-monotonic in protein-cargo affinity, such affinity can be detrimental to assembly if it becomes strong enough to stabilize frustrated intermediates that are incompatible with the ground state structure. In cases where the subunits are capable of assembly into different morphologies, we find that maintaining the precise spatial arrangement of subunits seen in the crystal structure is possible even if non-native interactions are disfavored by as little as the thermal energy.

Many-molecule encapsulation by an icosahedral shell

PubMed Central

Perlmutter, Jason D; Mohajerani, Farzaneh; Hagan, Michael F

2016-01-01

We computationally study how an icosahedral shell assembles around hundreds of molecules. Such a process occurs during the formation of the carboxysome, a bacterial microcompartment that assembles around many copies of the enzymes ribulose 1,5-bisphosphate carboxylase/ oxygenase and carbonic anhydrase to facilitate carbon fixation in cyanobacteria. Our simulations identify two classes of assembly pathways leading to encapsulation of many-molecule cargoes. In one, shell assembly proceeds concomitantly with cargo condensation. In the other, the cargo first forms a dense globule; then, shell proteins assemble around and bud from the condensed cargo complex. Although the model is simplified, the simulations predict intermediates and closure mechanisms not accessible in experiments, and show how assembly can be tuned between these two pathways by modulating protein interactions. In addition to elucidating assembly pathways and critical control parameters for microcompartment assembly, our results may guide the reengineering of viruses as nanoreactors that self-assemble around their reactants. DOI: http://dx.doi.org/10.7554/eLife.14078.001 PMID:27166515

Reducing Mission Logistics with Multipurpose Cargo Transfer Bags

NASA Technical Reports Server (NTRS)

Baccus, Shelley; Broyan, James Lee, Jr.; Borrego, Melissa

2016-01-01

The Logistics Reduction (LR) project within Advanced Exploration Systems (AES) is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag (CTB), the common logistics carrying bag for the International Space Station (ISS). After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unfolded into a flat panel for reuse. Concepts and potential benefits for various MCTB applications will be discussed including partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing. Acoustic MCTBs are currently in use on ISS to reduce the noise generated by the T2 treadmill, which reaches the hazard limit at high speeds. The development of the AMCTB included identification of keep-out zones, acoustic properties, deployment considerations, and structural testing. Features developed for these considerations are applicable to MCTBs for all crew outfitting applications.

Multipurpose Cargo Transfer Bags fro Reducing Exploration Mission Logistics

NASA Technical Reports Server (NTRS)

Baccus, Shelley; Broyan, James Lee, Jr.; Borrego, Melissa

2016-01-01

The Logistics Reduction (LR) project within the Advanced Exploration Systems (AES) division is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag (CTB), the common logistics carrying bag for the International Space Station (ISS). After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unfolded into a flat panel for reuse. Concepts and potential benefits for various MCTB applications will be discussed including partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing. Acoustic MCTBs are currently in use on ISS to reduce the noise generated by the T2 treadmill, which reaches the hazard limit at high speeds. The development of the AMCTB included identification of keep out zones, acoustic properties, deployment considerations, and structural testing. Features developed for these considerations are applicable to MCTBs for all crew outfitting applications.

Harnessing catalytic pumps for directional delivery of microparticles in microchambers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Das, Sambeeta; Shklyaev, Oleg E.; Altemose, Alicia

The directed transport of microparticles in microfluidic devices is vital for efficient bioassays and fabrication of complex microstructures. There remains, but, a need for methods to propel and steer microscopic cargo that do not require modifying these particles. By using theory and experiments, we show that catalytic surface reactions can be used to deliver microparticle cargo to specified regions in microchambers. Here reagents diffuse from a gel reservoir and react with the catalyst-coated surface. Fluid density gradients due to the spatially varying reagent concentration induce a convective flow, which carries the suspended particles until the reagents are consumed. Consequently, themore » cargo is deposited around a specific position on the surface. The velocity and final peak location of the cargo can be tuned independently. And by increasing the local particle concentration, highly sensitive assays can be performed efficiently and rapidly. Moreover, the process can be repeated by introducing fresh reagent into the microchamber.« less

Harnessing catalytic pumps for directional delivery of microparticles in microchambers

DOE PAGES

Das, Sambeeta; Shklyaev, Oleg E.; Altemose, Alicia; ...

2017-02-17

The directed transport of microparticles in microfluidic devices is vital for efficient bioassays and fabrication of complex microstructures. There remains, but, a need for methods to propel and steer microscopic cargo that do not require modifying these particles. By using theory and experiments, we show that catalytic surface reactions can be used to deliver microparticle cargo to specified regions in microchambers. Here reagents diffuse from a gel reservoir and react with the catalyst-coated surface. Fluid density gradients due to the spatially varying reagent concentration induce a convective flow, which carries the suspended particles until the reagents are consumed. Consequently, themore » cargo is deposited around a specific position on the surface. The velocity and final peak location of the cargo can be tuned independently. And by increasing the local particle concentration, highly sensitive assays can be performed efficiently and rapidly. Moreover, the process can be repeated by introducing fresh reagent into the microchamber.« less

Survey of Fire Detection Technologies and System Evaluation/Certification Methodologies and Their Suitability for Aircraft Cargo Compartments

NASA Technical Reports Server (NTRS)

Cleary, T.; Grosshandler, W.

1999-01-01

As part of the National Aeronautics and Space Administration (NASA) initiated program on global civil aviation, NIST is assisting Federal Aviation Administration in its research to improve fire detection in aircraft cargo compartments. Aircraft cargo compartment detection certification methods have been reviewed. The Fire Emulator-Detector Evaluator (FE/DE) has been designed to evaluate fire detection technologies such as new sensors, multi-element detectors, and detectors that employ complex algorithms. The FE/DE is a flow tunnel that can reproduce velocity, temperature, smoke, and Combustion gas levels to which a detector might be exposed during a fire. A scientific literature survey and patent search have been conducted relating to existing and emerging fire detection technologies, and the potential use of new fire detection strategies in cargo compartment areas has been assessed. In the near term, improved detector signal processing and multi-sensor detectors based on combinations of smoke measurements, combustion gases and temperature are envisioned as significantly impacting detector system performance.

Raffaello is offloaded from a Beluga super transporter

NASA Technical Reports Server (NTRS)

1999-01-01

At the Shuttle Landing Facility, the one-piece, upward-hinged main cargo door of the Airbus Industrie A300-600ST 'Beluga' Super Transporter is open to offload its cargo, the second Multi-Purpose Logistics Module (MPLM) for the International Space Station (ISS). One of Italy's major contributions to the ISS program, the MPLM, named Raffaello, is a reusable logistics carrier and the primary delivery system used to resupply and return station cargo requiring a pressurized environment. Weighing nearly 4.5 tons, the module measures 21 feet long and 15 feet in diameter. Raffaello will join Leonardo, the first Italian-built MPLM, in the Space Station Processing Facility for testing. NASA, Boeing, the Italian Space Agency and Alenia Aerospazio will provide engineering support.

A propagating ATPase gradient drives transport of surface-confined cellular cargo

NASA Astrophysics Data System (ADS)

Vecchiarelli, Anthony; Neuman, Keir; Mizuuchi, Kiyoshi

2014-03-01

The process of DNA segregation is of central importance for all organisms. Although eukaryotic mitosis is relatively well established, the most common mechanism employed for bacterial DNA segregation has been unclear. ParA ATPases form dynamic patterns on the bacterial nucleoid, to spatially organize plasmids, chromosomes and other large cellular cargo, but the force generating mechanism has been a source of controversy and debate. A dominant view proposes that ParA-mediated transport and cargo positioning occurs via a filament-based mechanism that resembles eukaryotic mitosis. Here we present direct evidence against such models. Our cell-free reconstitution supports a non-filament-based mode of transport that may be as widely found in nature as actin filaments and microtubules.

46 CFR 154.315 - Cargo pump and cargo compressor rooms.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pump and cargo compressor rooms. 154.315 Section... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Ship Arrangements § 154.315 Cargo pump and cargo compressor rooms. (a) Cargo pump rooms and cargo...

Contamination control and cleanliness level integrity for the Space Shuttle Orbiter PLB, payloads and facilities at KSC

NASA Technical Reports Server (NTRS)

Bartelson, D.

1984-01-01

The PLB, its cargo, and payload canister must satisfy the cleanliness requirements of visual clean (VC) level 1, 2, 3, or special as stated in NASA document SN-C-0005A. The specific level of cleanliness is chosen by the payload bay customer for their mission. During orbiter turnaround processing at KSC, the payload bay is exposed to the environments of the Orbiter Processing Facility (OPF) and the Payload Changeout Room (PCR). In supportive response to the orbiter payload bay/facility interface, it is necessary that the facility environment be controlled and monitored to protect the cleanliness/environmental integrity of the payload bay and its cargo. Techniques used to meet environmental requirements during orbiter processing are introduced.

Cytosolic delivery of multi-domain cargos by the N-terminus of Pasteurella multocida toxin.

PubMed

Clemons, Nathan C; Bannai, Yuka; Haywood, Elizabeth E; Xu, Yiting; Buschbach, James D; Ho, Mengfei; Wilson, Brenda A

2018-05-21

The zoonotic pathogen Pasteurella multocida produces a 146-kDa modular toxin (PMT) that enters host cells and manipulates intracellular signaling through action on its Gα-protein targets. The N-terminus of PMT (PMT-N) mediates cellular uptake through receptor-mediated endocytosis, followed by delivery of the C-terminal catalytic domain from acidic endosomes into the cytosol. The putative native cargo of PMT consists of a 710-residue polypeptide of three distinct modular subdomains (C1-C2-C3), where C1 contains a membrane localization domain (MLD), C2 has as-of-yet undefined function, and C3 catalyzes deamidation of a specific active-site glutamine residue in Gα-protein targets. However, whether the three cargo subdomains are delivered intact or undergo further proteolytic processing during or after translocation from the late endosome is unclear. Here, we demonstrate that PMT-N mediates delivery of its native C-terminal cargo as a single polypeptide, corresponding to C1-C2-C3, including the MLD, with no evidence of cleavage between subdomains. We show that PMT-N also delivers into the cytosol non-native GFP cargo, further supporting that the receptor-binding and translocation functions reside within PMT-N. Our findings further show that PMT-N can deliver C1-C2 alone but that the presence of C1-C2 is important for cytosolic delivery of the catalytic C3 subdomain by PMT-N. In addition, we further refine the minimum C3 domain required for intracellular activity as comprising residues 1105-1278. These findings reinforce that PMT-N serves as the cytosolic delivery vehicle for C-terminal cargo and demonstrate that its native cargo is delivered intact as C1-C2-C3. Copyright © 2018 American Society for Microbiology.

Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling

PubMed Central

Bouzat, Sebastián; Levi, Valeria; Bruno, Luciana

2012-01-01

In this work, we explored theoretically the transport of organelles driven along microtubules by molecular motors of opposed polarities using a stochastic model that considers a Langevin dynamics for the cargo, independent cargo-motor linkers and stepping motion for the motors. It has been recently proposed that the stiffness of the motor plays an important role when multiple motors collectively transport a cargo. Therefore, we considered in our model the recently reported values for the stiffness of the cargo-motor linker determined in living cells (∼0.01 pN/nm, [1]) which is significantly lower than the motor stiffness obtained in in vitro assays and used in previous studies. Our model could reproduce the multimodal velocity distributions and typical trajectory characteristics including the properties of the reversions in the overall direction of motion observed during melanosome transport along microtubules in Xenopus laevis melanophores. Moreover, we explored the contribution of the different motility states of the cargo-motor system to the different modes of the velocity distributions and could identify the microscopic mechanisms of transport leading to trajectories compatible with those observed in living cells. Finally, by changing the attachment and detachment rates, the model could reproduce the different velocity distributions observed during melanosome transport along microtubules in Xenopus laevis melanophores stimulated for aggregation and dispersion. Our analysis suggests that active tug-of-war processes with loose mechanical coupling can account for several aspects of cargo transport along microtubules in living cells. PMID:22952716

Evaluation of a Shuttle Derived Vehicle (SDV) for Cargo Transportation

NASA Technical Reports Server (NTRS)

Roman, Jose M.; Meacham, Stephen B.; Krupp, Donald R.; Threet, G. E.; Best, Joel; Davis, Stephan R.; Crumbly, Christopher; Olsen, Ronald A.; Engler, Leah M.; Garner, Tim

2005-01-01

In this new era of space exploration, a host of launch vehicles are being examined for possible use in transporting cargo and crew to low Earth orbit and beyond. Launch vehicles derived from the Space Shuttle Program (SSP), known as Shuttle Derived Vehicles (SDVs), are prime candidates for heavy-lift duty because of their potential to minimize non-recurring costs and because the Shuttle can leverage off proven high-performance flight systems with established ground and flight support. To determine the merits of SDVs, a detailed evaluation was performed. This evaluation included a trade study and risk assessment of options based on performance, safety reliability, cost, operations, and evolution. The purpose of this paper is to explain the approach, processes, and tools used to evaluate launch vehicles for heavy lift cargo transportation. The process included defining the trade space, characterizing the concepts, analyzing the systems, and scoring the options. The process also included a review by subject experts from NASA and industry to compare past and recent study data and assess the risks. A set of technical performance measures (TPMs) was generated based on the study requirements and constraints. Tools such as INTROS and POST were used to calculate performance, FIRST was used for prediction of reliability, and other software packages, both commercial and NASA-owned, were applied to study the trade space. By following a clear process and using the right tools a thorough assessment was performed. An SDV can be classified as either a side-mount vehicle (SMV) or an in-line vehicle OLV). An SMV is a Space Shuttle where the Orbiter is replaced by a cargo carrier. An ILV is comprised of a modified Shuttle External Tank (ET) with engines mounted to the bottom and cargo mounted atop. For both families of vehicles, Solid Rocket Boosters (SRBs) are attached to the ET. The first derivate of Shuttle is defined as the vehicle with minimum changes necessary to transform the Space Shuttle into an SDV. Deltas from the first derivate were also formulated to study more SDV options. Examples of deltas include replacing the SRBs with larger and/or more SRBs, adding an upper stage, increasing the size of the ET, changing the engines, and modifying the elements. Challenges for SDV range from tailoring infrastructure to meeting the exploration schedule. Although SDV is based on the Space Shuttle, it still includes development risk for designing and building a Cargo Carrier. There are also performance challenges in that Shuttle is not optimized for cargo-only missions, but it is a robust system built on reusability. Balancing the strengths and weaknesses of the Shuttle to meet Lunar and Mars mission objectives provides the framework for an informative trade study. SDV was carefully analyzed and the results of the study provide invaluable data for use in the new exploration initiative.

OA-7 Cargo Module Loading

NASA Image and Video Library

2017-02-07

In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, thousands of pounds of supplies, equipment and scientific research materials are prepared for loading aboard a Cygnus spacecraft's pressurized cargo module (PCM) for the Orbital ATK CRS-7 mission to the International Space Station. Scheduled to launch on March 19, 2017, the commercial resupply services mission will lift off atop a United Launch Alliance Atlas V rocket from Space launch Complex 41 at Cape Canaveral Air Force Station.

Dynamic Properties of the International Space Station throughout the Assembly Process

DTIC Science & Technology

1998-06-29

with the launch of the Russian-built FGB, or Functional Cargo Block (most fitting translation) atop a Russian Proton rocket in June of 1998. Phase Two...experiments were conducted in areas of plant growth, life sciences, and microgravity science. But more importantly, Mir-Shuttle operations presented...Configuration Rationale/Comments 30Jun 1998 1 A/R Russian Proton • Functional Cargo Block (FGB) • FGB is a self-supporting active vehicle. • It provides

Integrated payload and mission planning, phase 3. Volume 2: Logic/Methodology for preliminary grouping of spacelab and mixed cargo payloads

NASA Technical Reports Server (NTRS)

Rodgers, T. E.; Johnson, J. F.

1977-01-01

The logic and methodology for a preliminary grouping of Spacelab and mixed-cargo payloads is proposed in a form that can be readily coded into a computer program by NASA. The logic developed for this preliminary cargo grouping analysis is summarized. Principal input data include the NASA Payload Model, payload descriptive data, Orbiter and Spacelab capabilities, and NASA guidelines and constraints. The first step in the process is a launch interval selection in which the time interval for payload grouping is identified. Logic flow steps are then taken to group payloads and define flight configurations based on criteria that includes dedication, volume, area, orbital parameters, pointing, g-level, mass, center of gravity, energy, power, and crew time.

46 CFR 111.106-13 - Cargo handling devices or cargo pump rooms handling flammable or combustible cargoes.

Code of Federal Regulations, 2014 CFR

2014-10-01

... pierced by fixed lights, drive shafts, and pump-engine control rods, provided that the shafts and rods are... 46 Shipping 4 2014-10-01 2014-10-01 false Cargo handling devices or cargo pump rooms handling... OSVs § 111.106-13 Cargo handling devices or cargo pump rooms handling flammable or combustible cargoes...

Bird's eye view of the STS-7 cargo bay inside the VPF

NASA Technical Reports Server (NTRS)

1983-01-01

Bird's eye view of the STS-7 cargo bay inside the vertical processing facility during pre-move-to-pad preparations. From top to bottom (forward to aft), the payloads are West Germany/Rbb's SPAS-01 or shuttle pallet satellite; OSTA-2, the second shuttle experiment sponsored by NASA Office of Space and Terrestrial Applications; Palapa B-1, a second generation communications spacecraft designed and built for Indonesia and Anik C-2, a communications satellite owned and operated by Telesat of Canada.

Cargo Movement Operations System (CMOS). Increment II System Design Document, Final

DTIC Science & Technology

1990-08-02

NO [ ] COMMENT DISPOSITION: COMMENT STATUS: OPEN [ ] CLOSED [ ] ORIGINATOR CONTROL NUMBER: SSDD-0002 PROGRAM OFFICE CONTROL NUMBER: DATA ITEM DISCREPANCY WORKSHEET CDRL NUMBER: A002-06 DATE: 08/02/90 ORIGINATOR NAME: John J. Brassil OFFICE SYMBOL: SAIC TELEPHONE NUMBER: 272-2999 SUBSTANTIVE: X EDITORIAL: PAGE NUMBER: 35 PARA NUMBER: 4.2.1.3.1 COMMENT OR RECOMMENDED CHANGE: Add subordinate paragraphs to describe the 3-digit System Capabilities that are listed under System Administration in Appendix G. RATIONALE: Both Process Outbound Cargo

Effectiveness of the Civil Aviation Security Program.

DTIC Science & Technology

1978-03-31

Passenger Screening Results 12. Scope of Civil Aviation Security Program 13. Basic Policies 14. Explosives Detection Dog Teams 15. Explosives Detection... policies guiding the program recognize airline responsibilities for the safety of passengers, baggage and cargo in their care as well as for the...U *i * (U U Los -7 .cn cf) 1-4 ~~LL _m e- Hf LMU 0- u,-C -oL -ccJLL LII -~ LLIOL 0 _ CL. LLJ cr-L LCnIJ C ~ ~ CnCD C. ) &j 2ic- nc r JL AJ -L JC C.- L

Ultra-Trace and Vapor Detection of Explosives and Narcotics Finalist for R&D 100 Award

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ewing, Robert

An instrument more sensitive than a canine’s nose identifies explosives and narcotics vapors in real time and at levels previously undetectable than any other sampling technology. The instrument is one among five PNNL-developed technologies in the running for an R&D 100 Award. Known as VP-IDENT, the tool coupled with a mass spectrometer, is ideal for aviation security, cargo screening, and broader counter-terrorism and national security activities where discovering dangerous substances is of utmost importance. Listen as researcher Robert Ewing explains.

Ultra-Trace and Vapor Detection of Explosives and Narcotics Finalist for R&D 100 Award

ScienceCinema

Ewing, Robert

2018-06-13

An instrument more sensitive than a canine’s nose identifies explosives and narcotics vapors in real time and at levels previously undetectable than any other sampling technology. The instrument is one among five PNNL-developed technologies in the running for an R&D 100 Award. Known as VP-IDENT, the tool coupled with a mass spectrometer, is ideal for aviation security, cargo screening, and broader counter-terrorism and national security activities where discovering dangerous substances is of utmost importance. Listen as researcher Robert Ewing explains.

MPLM Donatello is offloaded at the SLF

NASA Technical Reports Server (NTRS)

2001-01-01

At the KSC Shuttle Landing Facility, the Italian Space Agency's Multi- Purpose Logistics Module Donatello begins rolling out of the Airbus '''Beluga''' air cargo plane that brought it from the factory of Alenia Aerospazio in Turin, Italy. The third of three for the International Space Station, the module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

MPLM Donatello is offloaded at the SLF

NASA Technical Reports Server (NTRS)

2001-01-01

At the KSC Shuttle Landing Facility, the Italian Space Agency's Multi- Purpose Logistics Module Donatello rolls out of the Airbus '''Beluga''' air cargo plane that brought it from the factory of Alenia Aerospazio in Turin, Italy. The third of three for the International Space Station, the module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

Kin5 Knockdown in Tetrahymena thermophila Using RNAi Blocks Cargo Transport of Gef1

PubMed Central

Awan, Aashir; Bell, Aaron J.; Satir, Peter

2009-01-01

A critical process that builds and maintains the eukaryotic cilium is intraflagellar transport (IFT). This process utilizes members of the kinesin-2 superfamily to transport cargo into the cilium (anterograde transport) and a dynein motor for the retrograde traffic. Using a novel RNAi knockdown method, we have analyzed the function of the homodimeric IFT kinesin-2, Kin5, in Tetrahymena ciliary transport. In RNAi transformants, Kin5 was severely downregulated and disappeared from the cilia, but cilia did not resorb, although tip structure was affected. After deciliation of the knockdown cell, cilia regrew and cells swam, which suggested that Kin5 is not responsible for the trafficking of axonemal precursors to build the cilium, but could be transporting molecules that act in ciliary signal transduction, such as guanine nucleotide exchange proteins (GEFs). Gef1 is a Tetrahymena ciliary protein, and current coimmunoprecipitation and immunofluorescence studies showed that it is absent in regrowing cilia of the knockdown cells lacking ciliary Kin5. We suggest that one important cargo of Kin5 is Gef1 and knockdown of Kin5 results in cell lethality. PMID:19290045

KSC-98pc1161

NASA Image and Video Library

1998-09-27

KENNEDY SPACE CENTER, FLA. -- The Shuttle Carrier Aircraft gently lands its piggyback cargo orbiter Atlantis at the Shuttle Landing Facility. Atlantis returns home after a 10-month stay in the Palmdale, CA, orbiter processing facility undergoing extensive inspections and modifications. They included several upgrades enabling it to support International Space Station missions, such as adding an external airlock for ISS docking missions and installing thinner, lighter thermal protection blankets for weight reduction which will allow it to haul heavier cargo. The flight from Palmdale included a fueling stop in Ft. Hood, TX, and overnight stay at Ft. Campbell, KY. Atlantis will undergo preparations in the Orbiter Processing Facility at KSC for its planned flight in June 1999

The STS-96 crew takes part in a Crew Equipment Interface Test at KSC

NASA Technical Reports Server (NTRS)

1999-01-01

In the Orbiter Processing Facility bay 1, STS-96 Mission Specialists Daniel Barry (M.D., Ph.D.), Valery Ivanovich Tokarev and Tamara E. Jernigan (Ph.D.) look into the payload bay of the orbiter Discovery. The STS-96 crew is at KSC for a Crew Equipment Interface Test. Other crew members participating are Commander Kent V. Rominger, Pilot Rick Douglas Husband, and Mission Specialists Ellen Ochoa (Ph.D.) and Julie Payette, with the Canadian Space Agency. The primary payload of STS-96 is the SPACEHAB Double Module. In addition, the Space Shuttle will carry unpressurized cargo such as the external Russian cargo crane known as STRELA; the Spacehab Oceaneering Space System Box (SHOSS), which is a logistics items carrier; and an ORU Transfer Device (OTD), a U.S.-built crane that will be stowed on the station for use during future ISS assembly missions. These cargo items will be stowed on the International Cargo Carrier, fitted inside the payload bay behind the SPACEHAB module. STS-96 is targeted for launch on May 24 from Launch Pad 39B.

The STS-96 crew takes part in a Crew Equipment Interface Test at KSC

NASA Technical Reports Server (NTRS)

1999-01-01

In the Orbiter Processing Facility bay 1, STS-96 Commander Kent V. Rominger and Mission Specialists Ellen Ochoa (Ph.D.) and Valery Ivanovich Tokarev pose inside the orbiter Discovery. The STS-96 crew is at KSC to take part in a Crew Equipment Interface Test. Other members participating are Pilot Rick Douglas Husband and Mission Specialists Tamara E. Jernigan (Ph.D.), Daniel Barry (M.D., Ph.D.) and Julie Payette, who is with the Canadian Space Agency. Tokarev represents the Russian Space Agency. The primary payload of STS-96 is the SPACEHAB Double Module. In addition, the Space Shuttle will carry unpressurized cargo such as the external Russian cargo crane known as STRELA; the Spacehab Oceaneering Space System Box (SHOSS), which is a logistics items carrier; and an ORU Transfer Device (OTD), a U.S.-built crane that will be stowed on the station for use during future ISS assembly missions. These cargo items will be stowed on the International Cargo Carrier, fitted inside the payload bay behind the SPACEHAB module. STS-96 is targeted for launch on May 24 from Launch Pad 39B.

Research on characteristics of radiated noise of large cargo ship in shallow water

NASA Astrophysics Data System (ADS)

Liu, Yongdong; Zhang, Liang

2017-01-01

With the rapid development of the shipping industry, the number of the world's ship is gradually increasing. The characteristics of the radiated noise of the ship are also of concern. Since the noise source characteristics of multichannel interference, the surface wave and the sea temperature microstructure and other reasons, the sound signal received in the time-frequency domain has varying characteristics. The signal of the radiated noise of the large cargo ship JOCHOH from horizontal hydrophone array in some shallow water of China is processed and analyzed in the summer of 2015, and the results show that a large cargo ship JOCHOH has a number of noise sources in the direction of the ship's bow and stern lines, such as host, auxiliary and propellers. The radiating sound waves generated by these sources do not meet the spherical wave law at lower frequency in the ocean, and its radiated noise has inherent spatial distribution, the variation characteristics of the radiated noise the large cargo ship in time and frequency domain are given. The research method and results are of particular importance.

Diffusion-Limited Cargo Loading of an Engineered Protein Container.

PubMed

Zschoche, Reinhard; Hilvert, Donald

2015-12-30

The engineered bacterial nanocompartment AaLS-13 is a promising artificial encapsulation system that exploits electrostatic interactions for cargo loading. In order to study its ability to take up and retain guests, a pair of fluorescent proteins was developed which allows spectroscopic determination of the extent of encapsulation by Förster resonance energy transfer (FRET). The encapsulation process is generally complete within a second, suggesting low energetic barriers for proteins to cross the capsid shell. Formation of intermediate aggregates upon mixing host and guest in vitro complicates capsid loading at low ionic strength, but can be sidestepped by increasing salt concentrations or diluting the components. Encapsulation of guests is completely reversible, and the position of the equilibrium is easily tuned by varying the ionic strength. These results, which challenge the notion that AaLS-13 is a continuous rigid shell, provide valuable information about cargo loading that will guide ongoing efforts to engineer functional host-guest complexes. Moreover, it should be possible to adapt the protein FRET pair described in this report to characterize functional capsid-cargo complexes generated by other encapsulation systems.

KSC-99pp0314

NASA Image and Video Library

1999-03-24

In the Orbiter Processing Facility bay 1, STS-96 Mission Specialist Daniel Barry, M.D., Ph.D., looks at one of the foot restraints used for extravehicular activity, or space walks. The STS-96 crew is at KSC to take part in a Crew Equipment Interface Test. The other crew members are Commander Kent V. Rominger, Pilot Rick Douglas Husband, and Mission Specialists Ellen Ochoa (Ph.D.), Tamara E. Jernigan (Ph.D.), Julie Payette and Valery Ivanovich Tokarev. Payette represents the Canadian Space Agency and Tokarev the Russian Space Agency. The primary payload of STS-96 is the SPACEHAB Double Module. In addition, the Space Shuttle will carry unpressurized cargo such as the external Russian cargo crane known as STRELA; the Spacehab Oceaneering Space System Box (SHOSS), which is a logistics items carrier; and an ORU Transfer Device (OTD), a U.S.-built crane that will be stowed on the station for use during future ISS assembly missions. These cargo items will be stowed on the International Cargo Carrier, fitted inside the payload bay behind the SPACEHAB module. STS-96 is targeted for launch on May 24 from Launch Pad 39B

46 CFR 280.2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

.... For purposes of this part only: (a) Commercial cargo means cargo other than military cargo and civilian preference cargo. (b) Military cargo means that cargo required to be carried on a U.S.-flag vessel... by law to be carried on a U.S.-flag vessel, including, but not limited to, cargo required to be...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abercrombie, Robert K; Buckner Jr, Dooley; Newton, David D

The Oak Ridge National Laboratory (ORNL) Weigh-In-Motion (WIM) system provides a portable and/or semi-portable means of accurately weighing vehicles and its cargo as each vehicle crosses the scales (while in motion), and determining (1) axle weights and (2) axle spacing for vehicles (for determination of Bridge Formula compliance), (3) total vehicle/cargo weight and (4) longitudinal center of gravity (for safety considerations). The WIM system can also weigh the above statically. Because of the automated nature of the WIM system, it eliminates the introduction of human errors caused by manual computations and data entry, adverse weather conditions, and stress. Individual vehiclesmore » can be weighed continuously at low speeds (approximately 3-10 mph) and at intervals of less than one minute. The ORNL WIM system operates and is integrated into the Bethel Jacobs Company Transportation Management and Information System (TMIS, a Radio-Frequency Identification [RFID] enabled information system). The integrated process is as follows: Truck Identification Number and Tare Weight are programmed into a RFID Tag. Handheld RFID devices interact with the RFID Tag, and Electronic Shipping Document is written to the RFID Tag. The RFID tag read by an RFID tower identifies the vehicle and its associated cargo, the specific manifest of radioactive debris for the uniquely identified vehicle. The weight of the cargo (in this case waste debris) is calculated from total vehicle weight information supplied from WIM to TMIS and is further processed into the Information System and kept for historical and archival purposes. The assembled data is the further process in downstream information systems where waste coordination activities at the Y-12 Environmental Management Waste Management Facility (EMWMF) are written to RFID Tag. All cycle time information is monitored by Transportation Operations and Security personnel.« less

Pricing of NASA Space Shuttle transportation system cargo

NASA Technical Reports Server (NTRS)

Hale, C. W.

1979-01-01

A two-part pricing policy is investigated as the most feasible method of pricing the transportation services to be provided by NASA's SSTS. Engineering cost estimates and a deterministic operating cost model generate a data base and develop a procedure for pricing the services of the SSTS. It is expected that the SSTS will have a monopoly on space material processing in areas of crystal growth, glass processing, metallurgical space applications, and biomedical processes using electrophoresis which will require efficient pricing. Pricing problems, the SSTS operating costs based on orbit elevation, number of launch sites, and number of flights, capital costs of the SSTS, research and development costs, allocation of joint transportation costs of the SSTS to a particular space processing activity, and rates for the SSTS are discussed. It is concluded that joint costs for commercial cargoes carried in the SSTS can be most usefully handled by making cost allocations based on proportionate capacity utilization.

46 CFR 150.130 - Loading a cargo on vessels carrying cargoes with which it is incompatible.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Loading a cargo on vessels carrying cargoes with which it is incompatible. 150.130 Section 150.130 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.130 Loading a cargo on vessels...

46 CFR 150.130 - Loading a cargo on vessels carrying cargoes with which it is incompatible.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Loading a cargo on vessels carrying cargoes with which it is incompatible. 150.130 Section 150.130 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.130 Loading a cargo on vessels...

46 CFR 150.130 - Loading a cargo on vessels carrying cargoes with which it is incompatible.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Loading a cargo on vessels carrying cargoes with which it is incompatible. 150.130 Section 150.130 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.130 Loading a cargo on vessels...

46 CFR 150.130 - Loading a cargo on vessels carrying cargoes with which it is incompatible.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Loading a cargo on vessels carrying cargoes with which it is incompatible. 150.130 Section 150.130 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.130 Loading a cargo on vessels...

46 CFR 97.12-1 - Bulk ores and similar cargoes.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Bulk ores and similar cargoes. 97.12-1 Section 97.12-1... OPERATIONS Cargo Stowage § 97.12-1 Bulk ores and similar cargoes. (a) The owners or operators of general cargo vessels which carry bulk cargoes such as ore, ore concentrates, and similar cargoes shall furnish...

Assessing the Impact of Electrostatic Drag on Processive Molecular Motor Transport.

PubMed

Smith, J Darby; McKinley, Scott A

2018-06-04

The bidirectional movement of intracellular cargo is usually described as a tug-of-war among opposite-directed families of molecular motors. While tug-of-war models have enjoyed some success, recent evidence suggests underlying motor interactions are more complex than previously understood. For example, these tug-of-war models fail to predict the counterintuitive phenomenon that inhibiting one family of motors can decrease the functionality of opposite-directed transport. In this paper, we use a stochastic differential equations modeling framework to explore one proposed physical mechanism, called microtubule tethering, that could play a role in this "co-dependence" among antagonistic motors. This hypothesis includes the possibility of a trade-off: weakly bound trailing molecular motors can serve as tethers for cargoes and processing motors, thereby enhancing motor-cargo run lengths along microtubules; however, this introduces a cost of processing at a lower mean velocity. By computing the small- and large-time mean-squared displacement of our theoretical model and comparing our results to experimental observations of dynein and its "helper protein" dynactin, we find some supporting evidence for microtubule tethering interactions. We extrapolate these findings to predict how dynein-dynactin might interact with the opposite-directed kinesin motors and introduce a criterion for when the trade-off is beneficial in simple systems.

Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs

PubMed Central

Dix, Carly I.; Soundararajan, Harish Chandra; Dzhindzhev, Nikola S.; Begum, Farida; Suter, Beat; Ohkura, Hiroyuki; Stephens, Elaine

2013-01-01

Microtubule-based transport mediates the sorting and dispersal of many cellular components and pathogens. However, the mechanisms by which motor complexes are recruited to and regulated on different cargos remain poorly understood. Here we describe a large-scale biochemical screen for novel factors associated with RNA localization signals mediating minus end–directed mRNA transport during Drosophila development. We identified the protein Lissencephaly-1 (Lis1) and found that minus-end travel distances of localizing transcripts are dramatically reduced in lis1 mutant embryos. Surprisingly, given its well-documented role in regulating dynein mechanochemistry, we uncovered an important requirement for Lis1 in promoting the recruitment of dynein and its accessory complex dynactin to RNA localization complexes. Furthermore, we provide evidence that Lis1 levels regulate the overall association of dynein with dynactin. Our data therefore reveal a critical role for Lis1 within the mRNA localization machinery and suggest a model in which Lis1 facilitates motor complex association with cargos by promoting the interaction of dynein with dynactin. PMID:23918939

Detection of cocaine in cargo containers by high-volume vapor sampling: field test at Port of Miami

NASA Astrophysics Data System (ADS)

Neudorfl, Pavel; Hupe, Michael; Pilon, Pierre; Lawrence, Andre H.; Drolet, Gerry; Su, Chih-Wu; Rigdon, Stephen W.; Kunz, Terry D.; Ulwick, Syd; Hoglund, David E.; Wingo, Jeff J.; Demirgian, Jack C.; Shier, Patrick

1997-02-01

The use of marine containers is a well known smuggling method for large shipments of drugs. Such containers present an ideal method of smuggling as the examination method is time consuming, difficult and expensive for the importing community. At present, various methods are being studied for screening containers which would allow to rapidly distinguish between innocent and suspicious cargo. Air sampling is one such method. Air is withdrawn for the inside of containers and analyzed for telltale vapors uniquely associated with the drug. The attractive feature of the technique is that the containers could be sampled without destuffing and opening, since air could be conveniently withdrawn via ventilation ducts. In the present paper, the development of air sampling methodology for the detection of cocaine hydrochloride will be discussed, and the results from a recent field test will be presented. The results indicated that vapors of cocaine and its decomposition product, ecgonidine methyl ester, could serve as sensitive indicators of the presence of the drug in the containers.

LC3/GABARAP family proteins: autophagy-(un)related functions.

PubMed

Schaaf, Marco B E; Keulers, Tom G; Vooijs, Marc A; Rouschop, Kasper M A

2016-12-01

From yeast to mammals, autophagy is an important mechanism for sustaining cellular homeostasis through facilitating the degradation and recycling of aged and cytotoxic components. During autophagy, cargo is captured in double-membraned vesicles, the autophagosomes, and degraded through lysosomal fusion. In yeast, autophagy initiation, cargo recognition, cargo engulfment, and vesicle closure is Atg8 dependent. In higher eukaryotes, Atg8 has evolved into the LC3/GABARAP protein family, consisting of 7 family proteins [LC3A (2 splice variants), LC3B, LC3C, GABARAP, GABARAPL1, and GABARAPL2]. LC3B, the most studied family protein, is associated with autophagosome development and maturation and is used to monitor autophagic activity. Given the high homology, the other LC3/GABARAP family proteins are often presumed to fulfill similar functions. Nevertheless, substantial evidence shows that the LC3/GABARAP family proteins are unique in function and important in autophagy-independent mechanisms. In this review, we discuss the current knowledge and functions of the LC3/GABARAP family proteins. We focus on processing of the individual family proteins and their role in autophagy initiation, cargo recognition, vesicle closure, and trafficking, a complex and tightly regulated process that requires selective presentation and recruitment of these family proteins. In addition, functions unrelated to autophagy of the LC3/GABARAP protein family members are discussed.-Schaaf, M. B. E., Keulers, T. G, Vooijs, M. A., Rouschop, K. M. A. LC3/GABARAP family proteins: autophagy-(un)related functions. © FASEB.

KSC-2012-2511

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians load cargo into the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2513

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians load cargo into the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2512

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians load cargo into the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2510

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, preparations are under way to load cargo into the Space Exploration Technologies Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2514

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians stow cargo in the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2516

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, a cargo bag slides through the docking ring into the Space Exploration Technologies Dragon capsule for stowage for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Local synaptic signaling enhances the stochastic transport of motor-driven cargo in neurons

NASA Astrophysics Data System (ADS)

Newby, Jay; Bressloff, Paul C.

2010-09-01

The tug-of-war model of motor-driven cargo transport is formulated as an intermittent trapping process. An immobile trap, representing the cellular machinery that sequesters a motor-driven cargo for eventual use, is located somewhere within a microtubule track. A particle representing a motor-driven cargo that moves randomly with a forward bias is introduced at the beginning of the track. The particle switches randomly between a fast moving phase and a slow moving phase. When in the slow moving phase, the particle can be captured by the trap. To account for the possibility that the particle avoids the trap, an absorbing boundary is placed at the end of the track. Two local signaling mechanisms—intended to improve the chances of capturing the target—are considered by allowing the trap to affect the tug-of-war parameters within a small region around itself. The first is based on a localized adenosine triphosphate (ATP) concentration gradient surrounding a synapse, and the second is based on a concentration of tau—a microtubule-associated protein involved in Alzheimer's disease—coating the microtubule near the synapse. It is shown that both mechanisms can lead to dramatic improvements in the capture probability, with a minimal increase in the mean capture time. The analysis also shows that tau can cause a cargo to undergo random oscillations, which could explain some experimental observations.

A quantitative examination of the role of cargo-exerted forces in axonal transport

PubMed Central

Mitchell, Cassie S.; Lee, Robert H.

2009-01-01

Axonal transport, via molecular motors kinesin and dynein, is a critical process in supplying the necessary constituents to maintain normal neuronal function. In this study, we predict the role of cooperativity by motors of the same polarity across the entire spectrum of physiological axonal transport. That is, we examined how the number of motors, either kinesin or dynein, working together to move a cargo, results in the experimentally determined velocity profiles seen in fast and slow anterograde and retrograde transport. We quantified the physiological forces exerted on a motor by a cargo as a function of cargo size, transport velocity, and transport type. Our results show that the force exerted by our base case neurofilament (DNF=10nm, LNF=1.6μm) is ~1.25pN at 600nm/s; additionally, the force exerted by our base case organelle (DOrg=1μm) at 1,000nm/s is ~5.7pN. Our results indicate that while a single motor can independently carry an average cargo, cooperativity is required to produce the experimental velocity profiles for fast transport. However, no cooperativity is required to produce the slow transport velocity profiles; thus, a single dynein or kinesin can carry the average neurofilament retrogradely or anterogradely, respectively. The potential role cooperativity may play in the hypothesized mechanisms of motoneuron transport diseases such as Amyotrophic Lateral Sclerosis (ALS) is discussed. PMID:19150364

Cargo-shell and cargo-cargo couplings govern the mechanics of artificially loaded virus-derived cages

NASA Astrophysics Data System (ADS)

Llauró, Aida; Luque, Daniel; Edwards, Ethan; Trus, Benes L.; Avera, John; Reguera, David; Douglas, Trevor; Pablo, Pedro J. De; Castón, José R.

2016-04-01

Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two types of proteinaceous payloads. While bound cargo to the inner capsid surface mechanically reinforced the capsid in a structural manner, unbound cargo diffusing freely within the shell cavity pressurized the cages up to ~30 atm due to steric effects. Strong cargo-cargo coupling reduces the resilience of these nanocompartments in ~20% when bound to the shell. Understanding the stability of artificially loaded nanocages will help to design more robust and durable molecular nanocontainers.Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two types of proteinaceous payloads. While bound cargo to the inner capsid surface mechanically reinforced the capsid in a structural manner, unbound cargo diffusing freely within the shell cavity pressurized the cages up to ~30 atm due to steric effects. Strong cargo-cargo coupling reduces the resilience of these nanocompartments in ~20% when bound to the shell. Understanding the stability of artificially loaded nanocages will help to design more robust and durable molecular nanocontainers. Electronic supplementary information (ESI) available: 6 figures, 3 tables and theory. See DOI: 10.1039/c6nr01007e

Biological Activities of Extracellular Vesicles and Their Cargos from Bovine and Human Milk in Humans and Implications for Infants.

PubMed

Zempleni, Janos; Aguilar-Lozano, Ana; Sadri, Mahrou; Sukreet, Sonal; Manca, Sonia; Wu, Di; Zhou, Fang; Mutai, Ezra

2017-01-01

Extracellular vesicles (EVs) in milk harbor a variety of compounds, including lipids, proteins, noncoding RNAs, and mRNAs. Among the various classes of EVs, exosomes are of particular interest, because cargo sorting in exosomes is a regulated, nonrandom process and exosomes play essential roles in cell-to-cell communication. Encapsulation in exosomes confers protection against enzymatic and nonenzymatic degradation of cargos and provides a pathway for cellular uptake of cargos by endocytosis of exosomes. Compelling evidence suggests that exosomes in bovine milk are transported by intestinal cells, vascular endothelial cells, and macrophages in human and rodent cell cultures, and bovine-milk exosomes are delivered to peripheral tissues in mice. Evidence also suggests that cargos in bovine-milk exosomes, in particular RNAs, are delivered to circulating immune cells in humans. Some microRNAs and mRNAs in bovine-milk exosomes may regulate the expression of human genes and be translated into protein, respectively. Some exosome cargos are quantitatively minor in the diet compared with endogenous synthesis. However, noncanonical pathways have been identified through which low concentrations of dietary microRNAs may alter gene expression, such as the accumulation of exosomes in the immune cell microenvironment and the binding of microRNAs to Toll-like receptors. Phenotypes observed in infant-feeding studies include higher Mental Developmental Index, Psychomotor Development Index, and Preschool Language Scale-3 scores in breastfed infants than in those fed various formulas. In mice, supplementation with plant-derived MIR-2911 improved the antiviral response compared with controls. Porcine-milk exosomes promote the proliferation of intestinal cells in mice. This article discusses the above-mentioned advances in research concerning milk exosomes and their cargos in human nutrition. Implications for infant nutrition are emphasized, where permitted, but data in infants are limited. © 2017 American Society for Nutrition.

46 CFR 151.13-1 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-1 General. This subpart prescribes the requirements for cargo segregation for cargo tanks. These requirements are based on considerations of cargo...

46 CFR 151.13-1 - General.

Code of Federal Regulations, 2011 CFR

2011-10-01

... HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-1 General. This subpart prescribes the requirements for cargo segregation for cargo tanks. These requirements are based on considerations of cargo...

Internal Cargo Integration

NASA Technical Reports Server (NTRS)

Hart, Angela

2006-01-01

A description of internal cargo integration is presented. The topics include: 1) Typical Cargo for Launch/Disposal; 2) Cargo Delivery Requirements; 3) Cargo Return Requirements; and 4) Vehicle On-Orbit Stay Time.

Profiling extracellular vesicle release by the filarial nematode Brugia malayi reveals sex-specific differences in cargo and a sensitivity to ivermectin

PubMed Central

Harischandra, Hiruni; Yuan, Wang; Zamanian, Mostafa

2018-01-01

The filarial nematode Brugia malayi is an etiological agent of Lymphatic Filariasis. The capability of B. malayi and other parasitic nematodes to modulate host biology is recognized but the mechanisms by which such manipulation occurs are obscure. An emerging paradigm is the release of parasite-derived extracellular vesicles (EV) containing bioactive proteins and small RNA species that allow secretion of parasite effector molecules and their potential trafficking to host tissues. We have previously described EV release from the infectious L3 stage B. malayi and here we profile vesicle release across all intra-mammalian life cycle stages (microfilariae, L3, L4, adult male and female worms). Nanoparticle Tracking Analysis was used to quantify and size EVs revealing discrete vesicle populations and indicating a secretory process that is conserved across the life cycle. Brugia EVs are internalized by murine macrophages with no preference for life stage suggesting a uniform mechanism for effector molecule trafficking. Further, the use of chemical uptake inhibitors suggests all life stage EVs are internalized by phagocytosis. Proteomic profiling of adult male and female EVs using nano-scale LC-MS/MS described quantitative and qualitative differences in the adult EV proteome, helping define the biogenesis of Brugia EVs and revealing sexual dimorphic characteristics in immunomodulatory cargo. Finally, ivermectin was found to rapidly inhibit EV release by all Brugia life stages. Further this drug effect was also observed in the related filarial nematode, the canine heartworm Dirofilaria immitis but not in an ivermectin-unresponsive field isolate of that parasite, highlighting a potential mechanism of action for this drug and suggesting new screening platforms for anti-filarial drug development. PMID:29659599

46 CFR 150.120 - Definition of incompatible cargoes.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Definition of incompatible cargoes. 150.120 Section 150.120 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.120 Definition of incompatible cargoes. Except as described in § 150.150, a cargo...

46 CFR 150.120 - Definition of incompatible cargoes.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Definition of incompatible cargoes. 150.120 Section 150.120 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.120 Definition of incompatible cargoes. Except as described in § 150.150, a cargo...

46 CFR 150.120 - Definition of incompatible cargoes.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Definition of incompatible cargoes. 150.120 Section 150.120 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.120 Definition of incompatible cargoes. Except as described in § 150.150, a cargo...

46 CFR 150.120 - Definition of incompatible cargoes.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Definition of incompatible cargoes. 150.120 Section 150.120 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.120 Definition of incompatible cargoes. Except as described in § 150.150, a cargo...

46 CFR 150.120 - Definition of incompatible cargoes.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Definition of incompatible cargoes. 150.120 Section 150.120 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.120 Definition of incompatible cargoes. Except as described in § 150.150, a cargo...

46 CFR 151.13-5 - Cargo segregation-tanks.

Code of Federal Regulations, 2014 CFR

2014-10-01

... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-5 Cargo segregation—tanks. (a... through design. (2) Segregation of cargo space from machinery spaces and other spaces which have or could... Grade E Liquid (if compatible with cargo) is satisfactory. (b) [Reserved] (c) If a cofferdam is required...

46 CFR 151.13-5 - Cargo segregation-tanks.

Code of Federal Regulations, 2013 CFR

2013-10-01

... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-5 Cargo segregation—tanks. (a... through design. (2) Segregation of cargo space from machinery spaces and other spaces which have or could... Grade E Liquid (if compatible with cargo) is satisfactory. (b) [Reserved] (c) If a cofferdam is required...

49 CFR 392.9 - Inspection of cargo, cargo securement devices and systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS DRIVING OF COMMERCIAL MOTOR VEHICLES General § 392.9 Inspection of cargo, cargo securement devices... drives that commercial motor vehicle; (2) Inspect the cargo and the devices used to secure the cargo...

33 CFR 105.265 - Security measures for handling cargo.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., containers, or other cargo transport units entering the facility match the delivery note or equivalent cargo..., containers or other cargo transport units, and cargo storage areas within the facility for evidence of... cargo. 105.265 Section 105.265 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND...

33 CFR 105.265 - Security measures for handling cargo.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., containers, or other cargo transport units entering the facility match the delivery note or equivalent cargo..., containers or other cargo transport units, and cargo storage areas within the facility for evidence of... cargo. 105.265 Section 105.265 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND...

33 CFR 105.265 - Security measures for handling cargo.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., containers, or other cargo transport units entering the facility match the delivery note or equivalent cargo..., containers or other cargo transport units, and cargo storage areas within the facility for evidence of... cargo. 105.265 Section 105.265 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND...

33 CFR 105.265 - Security measures for handling cargo.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., containers, or other cargo transport units entering the facility match the delivery note or equivalent cargo..., containers or other cargo transport units, and cargo storage areas within the facility for evidence of... cargo. 105.265 Section 105.265 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND...

33 CFR 105.265 - Security measures for handling cargo.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., containers, or other cargo transport units entering the facility match the delivery note or equivalent cargo..., containers or other cargo transport units, and cargo storage areas within the facility for evidence of... cargo. 105.265 Section 105.265 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND...

46 CFR 154.235 - Cargo tank location.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank location. 154.235 Section 154.235 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY STANDARDS... Survival Capability and Cargo Tank Location § 154.235 Cargo tank location. (a) For type IG hulls, cargo...

ADP SYSTEMS ANALYSIS - COMMITTED VS. AVAILABLE MILITARY TRANSPORTATION (LMI T1).

DTIC Science & Technology

LOGISTICS , * MANAGEMENT ENGINEERING), (*DATA PROCESSING, LOGISTICS), INFORMATION RETRIEVAL, SYSTEMS ENGINEERING, MILITARY TRANSPORTATION, CARGO VEHICLES, SCHEDULING, COMPUTER PROGRAMMING, MANAGEMENT PLANNING AND CONTROL

KSC-98pc1162

NASA Image and Video Library

1998-09-27

KENNEDY SPACE CENTER, FLA. -- The Shuttle Carrier Aircraft rolls to a stop with its piggyback cargo orbiter Atlantis at the Shuttle Landing Facility. In the background is the Vehicle Assembly Building. Atlantis returns home after a 10-month stay in the Palmdale, CA, orbiter processing facility undergoing extensive inspections and modifications. They included several upgrades enabling it to support International Space Station missions, such as adding an external airlock for ISS docking missions and installing thinner, lighter thermal protection blankets for weight reduction which will allow it to haul heavier cargo. The flight from Palmdale included a fueling stop in Ft. Hood, TX, and overnight stay at Ft. Campbell, KY. Atlantis will undergo preparations in the Orbiter Processing Facility at KSC for its planned flight in June 1999

KSC-98pc1163

NASA Image and Video Library

1998-09-27

KENNEDY SPACE CENTER, FLA. -- Stairs are rolled to the forward opening of the Shuttle Carrier Aircraft with its piggyback cargo, the orbiter Atlantis after it rolls to a stop at the Shuttle Landing Facility. Atlantis returns home after a 10-month stay in the Palmdale, CA, orbiter processing facility undergoing extensive inspections and modifications. They included several upgrades enabling it to support International Space Station missions, such as adding an external airlock for ISS docking missions and installing thinner, lighter thermal protection blankets for weight reduction which will allow it to haul heavier cargo. The flight from Palmdale included a fueling stop in Ft. Hood, TX, and overnight stay at Ft. Campbell, KY. Atlantis will undergo preparations in the Orbiter Processing Facility at KSC for its planned flight in June 1999

KSC-01pp0244

NASA Image and Video Library

2001-02-03

The lid is off the shipping container with the Multi-Purpose Logistics Module Donatello inside. It sits on a transporter inside the Space Station Processing Facility. In the SSPF, Donatello will undergo processing by the payload test team, including integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle’s payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo. Donatello will be launched on mission STS-130, currently planned for September 2004

KSC-01pp0245

NASA Image and Video Library

2001-02-03

Workers in the Space Station Processing Facility attach an overhead crane to the Multi-Purpose Logistics Module Donatello to lift it out of the shipping container. In the SSPF, Donatello will undergo processing by the payload test team, including integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle’s payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo. Donatello will be launched on mission STS-130, currently planned for September 2004

KSC-01pp0246

NASA Image and Video Library

2001-02-03

In the Space Station Processing Facility, workers help guide the overhead crane as it lifts the Multi-Purpose Logistics Module Donatello out of the shipping container. In the SSPF, Donatello will undergo processing by the payload test team, including integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle’s payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo. Donatello will be launched on mission STS-130, currently planned for September 2004

KSC-01pp0247

NASA Image and Video Library

2001-02-03

In the Space Station Processing Facility, workers help guide the Multi-Purpose Logistics Module Donatello as it moves the length of the SSPF toward a workstand. In the SSPF, Donatello will undergo processing by the payload test team, including integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle’s payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo. Donatello will be launched on mission STS-130, currently planned for September 2004

KSC-01pp0248

NASA Image and Video Library

2001-02-03

In the Space Station Processing Facility, workers wait for the Multi-Purpose Logistics Module Donatello, suspended by an overhead crane, to move onto a workstand. In the SSPF, Donatello will undergo processing by the payload test team, including integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle’s payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo. Donatello will be launched on mission STS-130, currently planned for September 2004

OA-7 Cargo Module Hatch Closure and Rotate to Vertical at SSPF

NASA Image and Video Library

2017-02-12

In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, the hatch is closed on the Cygnus spacecraft's pressurized cargo module (PCM) for the Orbital ATK CRS-7 mission to the International Space Station. The module is then rotated to vertical for mating to the service module. Scheduled to launch on March 19, 2017, the commercial resupply services mission will lift off atop a United Launch Alliance Atlas V rocket from Space launch Complex 41 at Cape Canaveral Air Force Station.

OA-7 Mate Service Module to Cargo Module

NASA Image and Video Library

2017-02-14

In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians and engineers mate a Cygnus spacecraft's pressurized cargo module to its service module. Cygnus is being prepared to deliver thousands of pounds of supplies, equipment and scientific research materials on the Orbital ATK CRS-7 mission to the International Space Station. Scheduled to launch on March 19, 2017, the commercial resupply services mission will lift off atop a United Launch Alliance Atlas V rocket from Space launch Complex 41 at Cape Canaveral Air Force Station.

46 CFR 154.476 - Cargo transfer devices and means.

Code of Federal Regulations, 2010 CFR

2010-10-01

... of cargo transfer, such as another pump or gas pressurization. (b) If cargo is transferred by gas... SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Support System § 154.476 Cargo transfer devices and means. (a) If a cargo pump in a cargo tank is...

46 CFR 154.1200 - Mechanical ventilation system: General.

Code of Federal Regulations, 2013 CFR

2013-10-01

...) Each cargo compressor room, pump room, gas-dangerous cargo control station, and space that contains... motors for cargo handling equipment. (2) Each gas-safe cargo control station in the cargo area. (3) Each...

46 CFR 154.1200 - Mechanical ventilation system: General.

Code of Federal Regulations, 2012 CFR

2012-10-01

...) Each cargo compressor room, pump room, gas-dangerous cargo control station, and space that contains... motors for cargo handling equipment. (2) Each gas-safe cargo control station in the cargo area. (3) Each...

46 CFR 154.1200 - Mechanical ventilation system: General.

Code of Federal Regulations, 2014 CFR

2014-10-01

...) Each cargo compressor room, pump room, gas-dangerous cargo control station, and space that contains... motors for cargo handling equipment. (2) Each gas-safe cargo control station in the cargo area. (3) Each...

46 CFR 151.20-15 - Cargo hose if carried on the barge.

Code of Federal Regulations, 2012 CFR

2012-10-01

... CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Transfer § 151.20-15 Cargo hose if carried on the barge. (a) Liquid and vapor line hose used for cargo transfer shall be of suitable material... subjected and shall be acceptable to the Commandant. (b) Hose subject to tank pressure, or the discharge...

46 CFR 151.20-15 - Cargo hose if carried on the barge.

Code of Federal Regulations, 2013 CFR

2013-10-01

... CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Transfer § 151.20-15 Cargo hose if carried on the barge. (a) Liquid and vapor line hose used for cargo transfer shall be of suitable material... subjected and shall be acceptable to the Commandant. (b) Hose subject to tank pressure, or the discharge...

46 CFR 151.20-15 - Cargo hose if carried on the barge.

Code of Federal Regulations, 2011 CFR

2011-10-01

... CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Transfer § 151.20-15 Cargo hose if carried on the barge. (a) Liquid and vapor line hose used for cargo transfer shall be of suitable material... subjected and shall be acceptable to the Commandant. (b) Hose subject to tank pressure, or the discharge...

46 CFR 151.20-15 - Cargo hose if carried on the barge.

Code of Federal Regulations, 2014 CFR

2014-10-01

... CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Transfer § 151.20-15 Cargo hose if carried on the barge. (a) Liquid and vapor line hose used for cargo transfer shall be of suitable material... subjected and shall be acceptable to the Commandant. (b) Hose subject to tank pressure, or the discharge...

46 CFR 151.05-2 - Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or...

Code of Federal Regulations, 2014 CFR

2014-10-01

... benzene and benzene containing cargoes, or butyl acrylate cargoes. 151.05-2 Section 151.05-2 Shipping... Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or butyl acrylate cargoes. A tank barge certificated to carry benzene and benzene containing cargoes or butyl...

46 CFR 151.05-2 - Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or...

Code of Federal Regulations, 2010 CFR

2010-10-01

... benzene and benzene containing cargoes, or butyl acrylate cargoes. 151.05-2 Section 151.05-2 Shipping... Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or butyl acrylate cargoes. A tank barge certificated to carry benzene and benzene containing cargoes or butyl...

46 CFR 151.05-2 - Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or...

Code of Federal Regulations, 2012 CFR

2012-10-01

... benzene and benzene containing cargoes, or butyl acrylate cargoes. 151.05-2 Section 151.05-2 Shipping... Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or butyl acrylate cargoes. A tank barge certificated to carry benzene and benzene containing cargoes or butyl...

46 CFR 151.05-2 - Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or...

Code of Federal Regulations, 2013 CFR

2013-10-01

... benzene and benzene containing cargoes, or butyl acrylate cargoes. 151.05-2 Section 151.05-2 Shipping... Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or butyl acrylate cargoes. A tank barge certificated to carry benzene and benzene containing cargoes or butyl...

46 CFR 151.05-2 - Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or...

Code of Federal Regulations, 2011 CFR

2011-10-01

... benzene and benzene containing cargoes, or butyl acrylate cargoes. 151.05-2 Section 151.05-2 Shipping... Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or butyl acrylate cargoes. A tank barge certificated to carry benzene and benzene containing cargoes or butyl...

46 CFR 153.908 - Cargo viscosity and melting point information; measuring cargo temperature during discharge...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Cargo viscosity and melting point information; measuring... Cargo viscosity and melting point information; measuring cargo temperature during discharge: Categories... lading, a written statement of the following: (1) For Category A or B NLS, the cargo's viscosity at 20 °C...

46 CFR 153.908 - Cargo viscosity and melting point information; measuring cargo temperature during discharge...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Cargo viscosity and melting point information; measuring... Cargo viscosity and melting point information; measuring cargo temperature during discharge: Categories... lading, a written statement of the following: (1) For Category A or B NLS, the cargo's viscosity at 20 °C...

46 CFR 153.908 - Cargo viscosity and melting point information; measuring cargo temperature during discharge...

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Cargo viscosity and melting point information; measuring... Cargo viscosity and melting point information; measuring cargo temperature during discharge: Categories... lading, a written statement of the following: (1) For Category A or B NLS, the cargo's viscosity at 20 °C...

14 CFR 121.583 - Carriage of persons without compliance with the passenger-carrying requirements of this part.

Code of Federal Regulations, 2013 CFR

2013-01-01

... confidential cargo; (v) The preservation of fragile or perishable cargo; (vi) Experiments on, or testing of, cargo containers or cargo handling devices; (vii) The operation of special equipment for loading or unloading cargo; and (viii) The loading or unloading of outsize cargo. (5) A person described in paragraph...

14 CFR 121.583 - Carriage of persons without compliance with the passenger-carrying requirements of this part.

Code of Federal Regulations, 2014 CFR

2014-01-01

... confidential cargo; (v) The preservation of fragile or perishable cargo; (vi) Experiments on, or testing of, cargo containers or cargo handling devices; (vii) The operation of special equipment for loading or unloading cargo; and (viii) The loading or unloading of outsize cargo. (5) A person described in paragraph...

Intentional cargo disruption by nefarious means: Examining threats, systemic vulnerabilities and securitisation measures in complex global supply chains.

PubMed

McGreevy, Conor; Harrop, Wayne

2015-01-01

Global trade and commerce requires products to be securely contained and transferred in a timely way across great distances and between national boundaries. Throughout the process, cargo and containers are stored, handled and checked by a range of authorities and authorised agents. Intermodal transportation involves the use of container ships, planes, railway systems, land bridges, road networks and barges. This paper examines the the nefarious nature of intentional disruption and nefarious risks associated with the movement of cargo and container freight. The paper explores main threats, vulnerabilities and security measures relevant to significant intermodal transit risk issues such as theft, piracy, terrorism, contamination, counterfeiting and product tampering. Three risk and vulnerability models are examined and basic standards and regulations that are relevant to safe and secure transit of container goods across international supply networks are outlined.

Microfluidic Droplet-Facilitated Hierarchical Assembly for Dual Cargo Loading and Synergistic Delivery.

PubMed

Yu, Ziyi; Zheng, Yu; Parker, Richard M; Lan, Yang; Wu, Yuchao; Coulston, Roger J; Zhang, Jing; Scherman, Oren A; Abell, Chris

2016-04-06

Bottom-up hierarchical assembly has emerged as an elaborate and energy-efficient strategy for the fabrication of smart materials. Herein, we present a hierarchical assembly process, whereby linear amphiphilic block copolymers are self-assembled into micelles, which in turn are accommodated at the interface of microfluidic droplets via cucurbit[8]uril-mediated host-guest chemistry to form supramolecular microcapsules. The monodisperse microcapsules can be used for simultaneous carriage of both organic (Nile Red) and aqueous-soluble (fluorescein isothiocyanate-dextran) cargo. Furthermore, the well-defined compartmentalized structure benefits from the dynamic nature of the supramolecular interaction and offers synergistic delivery of cargos with triggered release or through photocontrolled porosity. This demonstration of premeditated hierarchical assembly, where interactions from the molecular to microscale are designed, illustrates the power of this route toward accessing the next generation of functional materials and encapsulation strategies.

KSC-2012-2520

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians close the hatch of the Dragon capsule. The hatch was open for cargo to be stowed in the capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2521

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, the hatch of the Space Exploration Technologies Dragon capsule has been closed following stowage of cargo in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2519

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians prepare to close the hatch of the Dragon capsule. The hatch was open for cargo to be stowed in the capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Peroxisome protein import: a complex journey.

PubMed

Baker, Alison; Lanyon-Hogg, Thomas; Warriner, Stuart L

2016-06-15

The import of proteins into peroxisomes possesses many unusual features such as the ability to import folded proteins, and a surprising diversity of targeting signals with differing affinities that can be recognized by the same receptor. As understanding of the structure and function of many components of the protein import machinery has grown, an increasingly complex network of factors affecting each step of the import pathway has emerged. Structural studies have revealed the presence of additional interactions between cargo proteins and the PEX5 receptor that affect import potential, with a subtle network of cargo-induced conformational changes in PEX5 being involved in the import process. Biochemical studies have also indicated an interdependence of receptor-cargo import with release of unloaded receptor from the peroxisome. Here, we provide an update on recent literature concerning mechanisms of protein import into peroxisomes. © 2016 The Author(s).

X-ray cargo container inspection system with few-view projection imaging

NASA Astrophysics Data System (ADS)

Duan, Xinhui; Cheng, Jianping; Zhang, Li; Xing, Yuxiang; Chen, Zhiqiang; Zhao, Ziran

2009-01-01

An X-ray cargo inspection system with few-view projection imaging is developed for detecting contraband in air containers. This paper describes this developing inspection system, including its configuration and the process of inspection using three imaging modalities: digital radiography (DR), few view imaging and computed tomography (CT). The few-view imaging can provide 3D images with much faster scanning speed than CT and do great help to quickly locate suspicious cargo in a container. An algorithm to reconstruct tomographic images from severely sparse projection data of few-view imaging is discussed. A cooperative work manner of the three modalities is presented to make the inspection more convenient and effective. Numerous experiments of performance tests and modality comparison are performed on our system for inspecting air containers. Results demonstrate the effectiveness of our methods and implementation of few-view imaging in practical inspection systems.

An airport cargo inspection system based on X-ray and thermal neutron analysis (TNA).

PubMed

Ipe, Nisy E; Akery, A; Ryge, P; Brown, D; Liu, F; Thieu, J; James, B

2005-01-01

A cargo inspection system incorporating a high-resolution X-ray imaging system with a material-specific detection system based on Ancore Corporation's patented thermal neutron analysis (TNA) technology can detect bulk quantities of explosives and drugs concealed in trucks or cargo containers. The TNA process utilises a 252Cf neutron source surrounded by a moderator. The neutron interactions with the inspected object result in strong and unique gamma-ray signals from nitrogen, which is a key ingredient in modern high explosives, and from chlorinated drugs. The TNA computer analyses the gamma-ray signals and automatically determines the presence of explosives or drugs. The radiation source terms and shielding design of the facility are described. For the X-ray generator, the primary beam, leakage radiation, and scattered primary and leakage radiation were considered. For the TNA, the primary neutrons and tunnel scattered neutrons as well as the neutron-capture gamma rays were considered.

KSC-2012-2517

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, a Space Exploration Technologies technician attaches a cargo bag to the crane that will lift it toward the Dragon capsule where it will be stowed in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2515

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, a cargo bag is lowered into the hands of a Space Exploration Technologies technician who will load it into the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

A thermodynamic and heat transfer model for LNG ageing during ship transportation. Towards an efficient boil-off gas management

NASA Astrophysics Data System (ADS)

Krikkis, Rizos N.

2018-06-01

A non-equilibrium thermodynamic and heat transfer model for LNG ageing during ship transportation has been developed based on experimental data. The measurements reveal that the liquid temperature remains nearly constant, whereas significant variations are observed for the gas temperature. The measurement of the liquid temperature along the tank height suggests that a small scale rollover phenomenon may have taken place in one cargo tank. A time dependent heat transfer mechanism has been considered by taking into account the temperature variations of the atmospheric air, the seawater and the cofferdam environment which affect the cargo tanks. An important finding is that the evaporation rate (boil-of rate) is forced to follow the fuel flow consumption profile imposed by the vessel's propulsion system in order to match the tank pressure and volume constraints. The theoretical model is favorably compared to a comprehensive set on per hour basis of on board measurements of cargo temperatures and pressures, recorded during laden voyages, providing a better understanding of the underlying processes involved. The dominant role of the fuel consumption on the evaporation rate may be utilized in order to devise an efficient cargo management strategy during the laden voyage.

STS-96 M.S. Dan Barry checks equipment during a CEIT

NASA Technical Reports Server (NTRS)

1999-01-01

In the Orbiter Processing Facility bay 1, STS-96 Mission Specialist Daniel Barry, M.D., Ph.D., looks at one of the foot restraints used for extravehicular activity, or space walks. The STS-96 crew is at KSC to take part in a Crew Equipment Interface Test. The other crew members are Commander Kent V. Rominger, Pilot Rick Douglas Husband, and Mission Specialists Ellen Ochoa (Ph.D.), Tamara E. Jernigan (Ph.D.), Julie Payette and Valery Ivanovich Tokarev. Payette represents the Canadian Space Agency and Tokarev the Russian Space Agency. The primary payload of STS- 96 is the SPACEHAB Double Module. In addition, the Space Shuttle will carry unpressurized cargo such as the external Russian cargo crane known as STRELA; the Spacehab Oceaneering Space System Box (SHOSS), which is a logistics items carrier; and an ORU Transfer Device (OTD), a U.S.-built crane that will be stowed on the station for use during future ISS assembly missions. These cargo items will be stowed on the International Cargo Carrier, fitted inside the payload bay behind the SPACEHAB module. STS-96 is targeted for launch on May 24 from Launch Pad 39B.

MPLM Donatello is offloaded at the SLF

NASA Technical Reports Server (NTRS)

2001-01-01

At the Shuttle Landing Facility, workers in cherry pickers (left and right) help direct the offloading of the Italian Space Agency's Multi- Purpose Logistics Module Donatello from the Airbus '''Beluga''' air cargo plane that brought it from the factory of Alenia Aerospazio in Turin, Italy. The third of three for the International Space Station, the module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

MPLM Donatello is offloaded at the SLF

NASA Technical Reports Server (NTRS)

2001-01-01

At the Shuttle Landing Facility, cranes are poised to help offload the Italian Space Agency's Multi- Purpose Logistics Module Donatello from the Airbus '''Beluga''' air cargo plane that brought it from the factory of Alenia Aerospazio in Turin, Italy. The third of three for the International Space Station, the module will be transported to the Space Station Processing Facility for processing. Among the activities for the payload test team are integrated electrical tests with other Station elements in the SSPF, leak tests, electrical and software compatibility tests with the Space Shuttle (using the Cargo Integrated Test equipment) and an Interface Verification Test once the module is installed in the Space Shuttle's payload bay at the launch pad. The most significant mechanical task to be performed on Donatello in the SSPF is the installation and outfitting of the racks for carrying the various experiments and cargo.

46 CFR 232.5 - Income Statement Accounts.

Code of Federal Regulations, 2012 CFR

2012-10-01

... as terminal operations, cargo equipment, fleet operations, cargo pooling agreements, container... revenue from pooling agreements, terminal services provided to others, and cargo handling services performed for others; cargo equipment rentals, and repairs to cargo equipment belonging to others; agency...

46 CFR 232.5 - Income Statement Accounts.

Code of Federal Regulations, 2011 CFR

2011-10-01

... as terminal operations, cargo equipment, fleet operations, cargo pooling agreements, container... revenue from pooling agreements, terminal services provided to others, and cargo handling services performed for others; cargo equipment rentals, and repairs to cargo equipment belonging to others; agency...

46 CFR 232.5 - Income Statement Accounts.

Code of Federal Regulations, 2014 CFR

2014-10-01

... as terminal operations, cargo equipment, fleet operations, cargo pooling agreements, container... revenue from pooling agreements, terminal services provided to others, and cargo handling services performed for others; cargo equipment rentals, and repairs to cargo equipment belonging to others; agency...

46 CFR 232.5 - Income Statement Accounts.

Code of Federal Regulations, 2013 CFR

2013-10-01

... as terminal operations, cargo equipment, fleet operations, cargo pooling agreements, container... revenue from pooling agreements, terminal services provided to others, and cargo handling services performed for others; cargo equipment rentals, and repairs to cargo equipment belonging to others; agency...

46 CFR 150.140 - Cargoes not listed in Table I or II.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargoes not listed in Table I or II. 150.140 Section 150.140 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.140 Cargoes not listed in Table I or II. A cargo of hazardous material not listed...

46 CFR 150.140 - Cargoes not listed in Table I or II.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Cargoes not listed in Table I or II. 150.140 Section 150.140 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.140 Cargoes not listed in Table I or II. A cargo of hazardous material not listed...

46 CFR 150.140 - Cargoes not listed in Table I or II.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Cargoes not listed in Table I or II. 150.140 Section 150.140 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.140 Cargoes not listed in Table I or II. A cargo of hazardous material not listed...

46 CFR 150.140 - Cargoes not listed in Table I or II.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Cargoes not listed in Table I or II. 150.140 Section 150.140 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.140 Cargoes not listed in Table I or II. A cargo of hazardous material not listed...

46 CFR 150.140 - Cargoes not listed in Table I or II.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Cargoes not listed in Table I or II. 150.140 Section 150.140 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.140 Cargoes not listed in Table I or II. A cargo of hazardous material not listed...

Mission control activity during STS-61 EVA

NASA Image and Video Library

1993-12-07

STS61-S-101 (8 Dec 1993) --- Astronaut Gregory J. Harbaugh, spacecraft communicator (CAPCOM), observes as two astronauts work through a lengthy period of extravehicular activity (EVA) in the cargo bay of the Earth-orbiting Space Shuttle Endeavour. Seen on the screen in the front of the flight control room, preparing to work with the Hubble Space Telescope's (HST) magnetometers, are astronauts F. Story Musgrave and Jeffrey A. Hoffman. Harbaugh stayed busy passing up flight controllers suggestions and directions during the record-breaking battery of in-space servicing sessions. Lead flight director Milt Heflin is partially visible at left edge of frame.

Cargo Movement Operations System (CMOS). Final Software Design Document for the Applications CSCI, Increment II

DTIC Science & Technology

1991-10-03

NO [ ] COMMENT DISPOSITION: COMMENT STATUS: OPEN [ CLOSED [] ORIGINATOR CONTROL NUMBER: SDDl-0002 PROGRAM OFFiCE CONTROL NUMBER: DATA ITEM DISCREPANCY WORKSHEET CDRL NUMBER: A006-0250L DATE: 10/03/11 ORIGINATOR NAME: Ronald J. Lacour OFFICE SYMBOL: SAIC TELEPHONE NUMBER: 272-2999 SUBSTANTIVE: X EDITORIAL: PAGE NUMBER: CU2A5040-19 PARA NUMBER: N/A COMMENT OR RECOMMENDED CHANGE: Add "STANDBY PAX" on the last page of this screen. RATIONALE: This redesigned page is for handling standby passengers. However, no mention of that is present.

Aviation System Analysis Capability Air Carrier Investment Model-Cargo

NASA Technical Reports Server (NTRS)

Johnson, Jesse; Santmire, Tara

1999-01-01

The purpose of the Aviation System Analysis Capability (ASAC) Air Cargo Investment Model-Cargo (ACIMC), is to examine the economic effects of technology investment on the air cargo market, particularly the market for new cargo aircraft. To do so, we have built an econometrically based model designed to operate like the ACIM. Two main drivers account for virtually all of the demand: the growth rate of the Gross Domestic Product (GDP) and changes in the fare yield (which is a proxy of the price charged or fare). These differences arise from a combination of the nature of air cargo demand and the peculiarities of the air cargo market. The net effect of these two factors are that sales of new cargo aircraft are much less sensitive to either increases in GDP or changes in the costs of labor, capital, fuel, materials, and energy associated with the production of new cargo aircraft than the sales of new passenger aircraft. This in conjunction with the relatively small size of the cargo aircraft market means technology improvements to the cargo aircraft will do relatively very little to spur increased sales of new cargo aircraft.

Maritime industry : cargo preference laws--estimated costs and effects

DOT National Transportation Integrated Search

1994-11-30

Cargo preference laws require that certain government-owned or government-financed cargo shipped internationally (between a U.S. port and a foreign port) be carried on U.S.-flag vessels. Cargo subject to these laws is known as preference cargo. This ...

Characterizing Complexity of Containerized Cargo X-ray Images

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Guangxing; Martz, Harry; Glenn, Steven

X-ray imaging can be used to inspect cargos imported into the United States. In order to better understand the performance of X-ray inspection systems, the X-ray characteristics (density, complexity) of cargo need to be quantified. In this project, an image complexity measure called integrated power spectral density (IPSD) was studied using both DNDO engineered cargos and stream-of-commerce (SOC) cargos. A joint distribution of cargo density and complexity was obtained. A support vector machine was used to classify the SOC cargos into four categories to estimate the relative fractions.

Reconstituting the motility of isolated intracellular cargoes.

PubMed

Hendricks, Adam G; Goldman, Yale E; Holzbaur, Erika L F

2014-01-01

Kinesin, dynein, and myosin transport intracellular cargoes including organelles, membrane-bound vesicles, and mRNA along the cytoskeleton. These motor proteins work collectively in teams to transport cargoes over long distances and navigate around obstacles in the cell. In addition, several types of motors often interact on the same cargo to allow bidirectional transport and switching between the actin and microtubule networks. To examine transport of native cargoes in a simplified in vitro system, techniques have been developed to isolate endogenous cargoes and reconstitute their motility. Isolated cargoes can be tracked and manipulated with high precision using total internal reflection fluorescence microscopy and optical trapping. Through use of native cargoes, we can examine vesicular transport in a minimal system while retaining endogenous motor stoichiometry and the biochemical and mechanical characteristics of both motor and cargo. © 2014 Elsevier Inc. All rights reserved.

33 CFR 126.17 - Permits required for handling designated dangerous cargo.

Code of Federal Regulations, 2013 CFR

2013-07-01

... designated dangerous cargo. 126.17 Section 126.17 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) WATERFRONT FACILITIES HANDLING OF DANGEROUS CARGO AT WATERFRONT FACILITIES § 126.17 Permits required for handling designated dangerous cargo. Designated dangerous cargo may be...

46 CFR 148.72 - Dangerous cargo manifest; exceptions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Dangerous cargo manifest; exceptions. 148.72 Section 148.72 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DANGEROUS CARGOES CARRIAGE OF... Dangerous cargo manifest; exceptions. (a) No dangerous cargo manifest is required for— (1) Shipments by...

33 CFR 126.17 - Permits required for handling designated dangerous cargo.

Code of Federal Regulations, 2014 CFR

2014-07-01

... designated dangerous cargo. 126.17 Section 126.17 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) WATERFRONT FACILITIES HANDLING OF DANGEROUS CARGO AT WATERFRONT FACILITIES § 126.17 Permits required for handling designated dangerous cargo. Designated dangerous cargo may be...

46 CFR 148.72 - Dangerous cargo manifest; exceptions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Dangerous cargo manifest; exceptions. 148.72 Section 148.72 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DANGEROUS CARGOES CARRIAGE OF... Dangerous cargo manifest; exceptions. (a) No dangerous cargo manifest is required for— (1) Shipments by...

46 CFR 148.72 - Dangerous cargo manifest; exceptions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Dangerous cargo manifest; exceptions. 148.72 Section 148.72 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DANGEROUS CARGOES CARRIAGE OF... Dangerous cargo manifest; exceptions. (a) No dangerous cargo manifest is required for— (1) Shipments by...

33 CFR 126.17 - Permits required for handling designated dangerous cargo.

Code of Federal Regulations, 2012 CFR

2012-07-01

... designated dangerous cargo. 126.17 Section 126.17 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) WATERFRONT FACILITIES HANDLING OF DANGEROUS CARGO AT WATERFRONT FACILITIES § 126.17 Permits required for handling designated dangerous cargo. Designated dangerous cargo may be...

33 CFR 126.17 - Permits required for handling designated dangerous cargo.

Code of Federal Regulations, 2010 CFR

2010-07-01

... designated dangerous cargo. 126.17 Section 126.17 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) WATERFRONT FACILITIES HANDLING OF DANGEROUS CARGO AT WATERFRONT FACILITIES § 126.17 Permits required for handling designated dangerous cargo. Designated dangerous cargo may be...

46 CFR 148.72 - Dangerous cargo manifest; exceptions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Dangerous cargo manifest; exceptions. 148.72 Section 148.72 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DANGEROUS CARGOES CARRIAGE OF... Dangerous cargo manifest; exceptions. (a) No dangerous cargo manifest is required for— (1) Shipments by...

33 CFR 126.17 - Permits required for handling designated dangerous cargo.

Code of Federal Regulations, 2011 CFR

2011-07-01

... designated dangerous cargo. 126.17 Section 126.17 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) WATERFRONT FACILITIES HANDLING OF DANGEROUS CARGO AT WATERFRONT FACILITIES § 126.17 Permits required for handling designated dangerous cargo. Designated dangerous cargo may be...

46 CFR 153.235 - Exceptions to cargo piping location restrictions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Exceptions to cargo piping location restrictions. 153... DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Containment Systems § 153.235 Exceptions to cargo piping location restrictions...

46 CFR 153.235 - Exceptions to cargo piping location restrictions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Exceptions to cargo piping location restrictions. 153... DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Containment Systems § 153.235 Exceptions to cargo piping location restrictions...

46 CFR 151.01-15 - Dangerous cargoes not specifically named.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Dangerous cargoes not specifically named. 151.01-15 Section 151.01-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES General § 151.01-15 Dangerous cargoes not...

46 CFR 151.01-15 - Dangerous cargoes not specifically named.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Dangerous cargoes not specifically named. 151.01-15 Section 151.01-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES General § 151.01-15 Dangerous cargoes not...

46 CFR 151.01-15 - Dangerous cargoes not specifically named.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Dangerous cargoes not specifically named. 151.01-15 Section 151.01-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES General § 151.01-15 Dangerous cargoes not...

46 CFR 151.01-15 - Dangerous cargoes not specifically named.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Dangerous cargoes not specifically named. 151.01-15 Section 151.01-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES General § 151.01-15 Dangerous cargoes not...

46 CFR 151.01-15 - Dangerous cargoes not specifically named.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Dangerous cargoes not specifically named. 151.01-15 Section 151.01-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES General § 151.01-15 Dangerous cargoes not...

46 CFR 105.20-3 - Cargo tanks.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Cargo tanks. 105.20-3 Section 105.20-3 Shipping COAST... VESSELS DISPENSING PETROLEUM PRODUCTS Specific Requirements-Cargo Tanks § 105.20-3 Cargo tanks. (a) Construction and Materials. (1) The cargo tanks must be constructed of iron, steel, copper, nickel alloy...

46 CFR 105.20-3 - Cargo tanks.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Cargo tanks. 105.20-3 Section 105.20-3 Shipping COAST... VESSELS DISPENSING PETROLEUM PRODUCTS Specific Requirements-Cargo Tanks § 105.20-3 Cargo tanks. (a) Construction and Materials. (1) The cargo tanks must be constructed of iron, steel, copper, nickel alloy...

46 CFR 105.20-3 - Cargo tanks.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Cargo tanks. 105.20-3 Section 105.20-3 Shipping COAST... VESSELS DISPENSING PETROLEUM PRODUCTS Specific Requirements-Cargo Tanks § 105.20-3 Cargo tanks. (a) Construction and Materials. (1) The cargo tanks must be constructed of iron, steel, copper, nickel alloy...

46 CFR 105.20-3 - Cargo tanks.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Cargo tanks. 105.20-3 Section 105.20-3 Shipping COAST... VESSELS DISPENSING PETROLEUM PRODUCTS Specific Requirements-Cargo Tanks § 105.20-3 Cargo tanks. (a) Construction and Materials. (1) The cargo tanks must be constructed of iron, steel, copper, nickel alloy...

46 CFR 153.285 - Valving for cargo pump manifolds.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Valving for cargo pump manifolds. 153.285 Section 153... SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Piping Systems and Cargo Handling Equipment § 153.285 Valving for cargo pump manifolds. (a) When cargo...

46 CFR 153.438 - Cargo pressure or temperature alarms required.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Cargo pressure or temperature alarms required. 153.438... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Temperature Control Systems § 153.438 Cargo pressure or temperature alarms required. (a...

46 CFR 153.438 - Cargo pressure or temperature alarms required.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Cargo pressure or temperature alarms required. 153.438... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Temperature Control Systems § 153.438 Cargo pressure or temperature alarms required. (a...

46 CFR 153.438 - Cargo pressure or temperature alarms required.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Cargo pressure or temperature alarms required. 153.438... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Temperature Control Systems § 153.438 Cargo pressure or temperature alarms required. (a...

46 CFR 153.438 - Cargo pressure or temperature alarms required.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pressure or temperature alarms required. 153.438... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Temperature Control Systems § 153.438 Cargo pressure or temperature alarms required. (a...

46 CFR 153.438 - Cargo pressure or temperature alarms required.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Cargo pressure or temperature alarms required. 153.438... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Temperature Control Systems § 153.438 Cargo pressure or temperature alarms required. (a...

29 CFR 1918.87 - Ship's cargo elevators.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 7 2011-07-01 2011-07-01 false Ship's cargo elevators. 1918.87 Section 1918.87 Labor... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Handling Cargo § 1918.87 Ship's cargo elevators. (a) Safe working load. The safe working loads of ship's cargo elevators shall be determined and followed...

46 CFR 153.977 - Supervision of cargo transfer.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Supervision of cargo transfer. 153.977 Section 153.977 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Procedures § 153.977 Supervision of cargo transfer. The person in charge of cargo transfer shall: (a...

46 CFR 153.977 - Supervision of cargo transfer.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Supervision of cargo transfer. 153.977 Section 153.977 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Procedures § 153.977 Supervision of cargo transfer. The person in charge of cargo transfer shall: (a...

46 CFR 153.977 - Supervision of cargo transfer.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Supervision of cargo transfer. 153.977 Section 153.977 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Procedures § 153.977 Supervision of cargo transfer. The person in charge of cargo transfer shall: (a...

46 CFR 153.977 - Supervision of cargo transfer.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Supervision of cargo transfer. 153.977 Section 153.977 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Procedures § 153.977 Supervision of cargo transfer. The person in charge of cargo transfer shall: (a...

46 CFR 153.977 - Supervision of cargo transfer.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Supervision of cargo transfer. 153.977 Section 153.977 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Procedures § 153.977 Supervision of cargo transfer. The person in charge of cargo transfer shall: (a...

29 CFR 1918.87 - Ship's cargo elevators.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 7 2014-07-01 2014-07-01 false Ship's cargo elevators. 1918.87 Section 1918.87 Labor... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Handling Cargo § 1918.87 Ship's cargo elevators. (a) Safe working load. The safe working loads of ship's cargo elevators shall be determined and followed...

29 CFR 1918.87 - Ship's cargo elevators.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 7 2012-07-01 2012-07-01 false Ship's cargo elevators. 1918.87 Section 1918.87 Labor... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Handling Cargo § 1918.87 Ship's cargo elevators. (a) Safe working load. The safe working loads of ship's cargo elevators shall be determined and followed...

29 CFR 1918.87 - Ship's cargo elevators.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 7 2013-07-01 2013-07-01 false Ship's cargo elevators. 1918.87 Section 1918.87 Labor... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Handling Cargo § 1918.87 Ship's cargo elevators. (a) Safe working load. The safe working loads of ship's cargo elevators shall be determined and followed...

46 CFR 154.235 - Cargo tank location.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Cargo tank location. 154.235 Section 154.235 Shipping... FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Ship Survival Capability and Cargo Tank Location § 154.235 Cargo tank location. (a) For type IG hulls, cargo...

46 CFR 154.235 - Cargo tank location.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Cargo tank location. 154.235 Section 154.235 Shipping... FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Ship Survival Capability and Cargo Tank Location § 154.235 Cargo tank location. (a) For type IG hulls, cargo...

46 CFR 154.235 - Cargo tank location.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Cargo tank location. 154.235 Section 154.235 Shipping... FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Ship Survival Capability and Cargo Tank Location § 154.235 Cargo tank location. (a) For type IG hulls, cargo...

46 CFR 151.13-5 - Cargo segregation-tanks.

Code of Federal Regulations, 2010 CFR

2010-10-01

... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-5 Cargo segregation—tanks. (a... design. (2) Segregation of cargo space from machinery spaces and other spaces which have or could have a... separating medium. ii=Double bulkhead, required. Cofferdam, empty tank, pumproom, tank with Grade E Liquid...

46 CFR 151.13-5 - Cargo segregation-tanks.

Code of Federal Regulations, 2011 CFR

2011-10-01

... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-5 Cargo segregation—tanks. (a... design. (2) Segregation of cargo space from machinery spaces and other spaces which have or could have a... separating medium. ii=Double bulkhead, required. Cofferdam, empty tank, pumproom, tank with Grade E Liquid...

46 CFR 151.13-5 - Cargo segregation-tanks.

Code of Federal Regulations, 2012 CFR

2012-10-01

... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-5 Cargo segregation—tanks. (a... design. (2) Segregation of cargo space from machinery spaces and other spaces which have or could have a... separating medium. ii=Double bulkhead, required. Cofferdam, empty tank, pumproom, tank with Grade E Liquid...

46 CFR 154.701 - Cargo pressure and temperature control: General.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Cargo pressure and temperature control: General. 154.701... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Cargo Pressure and Temperature Control § 154.701 Cargo pressure and temperature control: General...

46 CFR 154.701 - Cargo pressure and temperature control: General.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Cargo pressure and temperature control: General. 154.701... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Cargo Pressure and Temperature Control § 154.701 Cargo pressure and temperature control: General...

46 CFR 154.701 - Cargo pressure and temperature control: General.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Cargo pressure and temperature control: General. 154.701... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Cargo Pressure and Temperature Control § 154.701 Cargo pressure and temperature control: General...

46 CFR 154.701 - Cargo pressure and temperature control: General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pressure and temperature control: General. 154.701... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Cargo Pressure and Temperature Control § 154.701 Cargo pressure and temperature control: General...

46 CFR 154.701 - Cargo pressure and temperature control: General.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Cargo pressure and temperature control: General. 154.701... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Cargo Pressure and Temperature Control § 154.701 Cargo pressure and temperature control: General...

46 CFR 308.545 - Facultative cargo policy, Form MA-316.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 308.545 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Iii-Facultative War Risk Cargo Insurance § 308.545 Facultative cargo policy, Form MA-316. The standard form of War Risk Facultative Cargo Policy, Form MA-316, may be obtained...

46 CFR 308.545 - Facultative cargo policy, Form MA-316.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 308.545 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Iii-Facultative War Risk Cargo Insurance § 308.545 Facultative cargo policy, Form MA-316. The standard form of War Risk Facultative Cargo Policy, Form MA-316, may be obtained...

46 CFR 308.511 - Cancellation of Open Cargo Policy.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Cancellation of Open Cargo Policy. 308.511 Section 308.511 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Open Policy War Risk Cargo Insurance § 308.511 Cancellation of Open Cargo...

46 CFR 308.545 - Facultative cargo policy, Form MA-316.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 308.545 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Iii-Facultative War Risk Cargo Insurance § 308.545 Facultative cargo policy, Form MA-316. The standard form of War Risk Facultative Cargo Policy, Form MA-316, may be obtained...

46 CFR 308.545 - Facultative cargo policy, Form MA-316.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 308.545 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Iii-Facultative War Risk Cargo Insurance § 308.545 Facultative cargo policy, Form MA-316. The standard form of War Risk Facultative Cargo Policy, Form MA-316, may be obtained...

46 CFR 308.545 - Facultative cargo policy, Form MA-316.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 308.545 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Facultative War Risk Cargo Insurance § 308.545 Facultative cargo policy, Form MA-316. The standard form of War Risk Facultative Cargo Policy, Form MA-316, may be obtained from...

46 CFR 153.907 - Cargo information.

Code of Federal Regulations, 2011 CFR

2011-10-01

... exposed to the cargo. (7) A list of fire fighting procedures and extinguishing agents effective with cargo fires. (8) Shipper's name. (9) Loading point. (10) Approximate quantity of cargo. (11) Tank in which the... of these two tables. (2) The name of the cargo prescribed in the letter authorizing carriage of the...

46 CFR 153.907 - Cargo information.

Code of Federal Regulations, 2014 CFR

2014-10-01

... exposed to the cargo. (7) A list of fire fighting procedures and extinguishing agents effective with cargo fires. (8) Shipper's name. (9) Loading point. (10) Approximate quantity of cargo. (11) Tank in which the... of these two tables. (2) The name of the cargo prescribed in the letter authorizing carriage of the...

46 CFR 153.907 - Cargo information.

Code of Federal Regulations, 2013 CFR

2013-10-01

... exposed to the cargo. (7) A list of fire fighting procedures and extinguishing agents effective with cargo fires. (8) Shipper's name. (9) Loading point. (10) Approximate quantity of cargo. (11) Tank in which the... of these two tables. (2) The name of the cargo prescribed in the letter authorizing carriage of the...

46 CFR 154.1831 - Persons in charge of transferring liquid cargo in bulk or preparing cargo tanks.

Code of Federal Regulations, 2011 CFR

2011-10-01

... in bulk or a cool-down, warm-up, gas-free, or air-out of each cargo tank; (2) Each transfer of liquid cargo in bulk, and each cool-down, warm-up, gas-free, or air-out of a cargo tank, is supervised by a... in bulk or a cool-down, warm-up, gas-free, or air-out of a cargo tank possesses the qualifications...

46 CFR 154.1831 - Persons in charge of transferring liquid cargo in bulk or preparing cargo tanks.

Code of Federal Regulations, 2014 CFR

2014-10-01

... in bulk or a cool-down, warm-up, gas-free, or air-out of each cargo tank; (2) Each transfer of liquid cargo in bulk, and each cool-down, warm-up, gas-free, or air-out of a cargo tank, is supervised by a... in bulk or a cool-down, warm-up, gas-free, or air-out of a cargo tank possesses the qualifications...

46 CFR 154.1831 - Persons in charge of transferring liquid cargo in bulk or preparing cargo tanks.

Code of Federal Regulations, 2012 CFR

2012-10-01

... in bulk or a cool-down, warm-up, gas-free, or air-out of each cargo tank; (2) Each transfer of liquid cargo in bulk, and each cool-down, warm-up, gas-free, or air-out of a cargo tank, is supervised by a... in bulk or a cool-down, warm-up, gas-free, or air-out of a cargo tank possesses the qualifications...

46 CFR 154.1831 - Persons in charge of transferring liquid cargo in bulk or preparing cargo tanks.

Code of Federal Regulations, 2013 CFR

2013-10-01

... in bulk or a cool-down, warm-up, gas-free, or air-out of each cargo tank; (2) Each transfer of liquid cargo in bulk, and each cool-down, warm-up, gas-free, or air-out of a cargo tank, is supervised by a... in bulk or a cool-down, warm-up, gas-free, or air-out of a cargo tank possesses the qualifications...

The promise of air cargo: System aspects and vehicle design

NASA Technical Reports Server (NTRS)

Whitehead, A. H., Jr.

1976-01-01

The current operation of the air cargo system is reviewed. An assessment of the future of air cargo is provided by: (1) analyzing statistics and trends, (2) by noting system problems and inefficiencies, (3) by analyzing characteristics of 'air eligible' commodities, and (4) by showing the promise of new technology for future cargo aircraft with significant improvements in costs and efficiency. The following topics are discussed: (1) air cargo demand forecasts; (2) economics of air cargo transport; (3) the integrated air cargo system; (4) evolution of airfreighter design; and (5) the span distributed load concept.

KSC-2009-4611

NASA Image and Video Library

2009-06-25

CAPE CANAVERAL, Fla. – The U.S. Air Force C-17 aircraft arrives at NASA Kennedy Space Center's Shuttle Landing Facility with its SV-1 cargo of the STSS Demonstrator spacecraft. The cargo will be transferred to the Astrotech payload processing facility in Titusville, Fla. The spacecraft is a midcourse tracking technology demonstrator, part of an evolving ballistic missile defense system. STSS is capable of tracking objects after boost phase and provides trajectory information to other sensors. It will be launched by NASA for the Missile Defense Agency in late summer. Photo credit: NASA/Kim Shiflett (Approved for Public Release 09-MDA-4804 [4 Aug 09] )

KSC-2009-4612

NASA Image and Video Library

2009-06-25

CAPE CANAVERAL, Fla. – At NASA Kennedy Space Center's Shuttle Landing Facility, the SV-1 cargo of the STSS Demonstrator spacecraft moves out of the U.S. Air Force C-17. The cargo will be transferred to the Astrotech payload processing facility in Titusville, Fla. The spacecraft is a midcourse tracking technology demonstrator, part of an evolving ballistic missile defense system. STSS is capable of tracking objects after boost phase and provides trajectory information to other sensors. It will be launched by NASA for the Missile Defense Agency in late summer. Photo credit: NASA/Kim Shiflett (Approved for Public Release 09-MDA-4804 [4 Aug 09] )

KSC-2009-4613

NASA Image and Video Library

2009-06-25

CAPE CANAVERAL, Fla. – At NASA Kennedy Space Center's Shuttle Landing Facility, the SV-1 cargo of the STSS Demonstrator spacecraft moves out of the U.S. Air Force C-17. The cargo will be transferred to the Astrotech payload processing facility in Titusville, Fla. The spacecraft is a midcourse tracking technology demonstrator, part of an evolving ballistic missile defense system. STSS is capable of tracking objects after boost phase and provides trajectory information to other sensors. It will be launched by NASA for the Missile Defense Agency in late summer. Photo credit: NASA/Kim Shiflett (Approved for Public Release 09-MDA-4804 [4 Aug 09] )

KSC-04PD-2309

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. In the Space Station Processing Facility, workers watch the progress of the Multi-Purpose Logistics Module Leonardo as it moves across the building to the Cargo Element Work Stand that Raffaello recently vacated. The payload canister was a temporary location during the switch. At right is the MPLM Raffaello, temporarily occupying the Element Rotation Stand formerly holding Leonardo. Three MPLMs were built by the Italian Space Agency Donatello, Leonardo and Raffaello to serve as a reusable logistics carrier and primary delivery system to resupply and return cargo requiring a pressurized environment to the International Space Station.

33 CFR 6.12-3 - Approval of facility for dangerous cargo.

Code of Federal Regulations, 2010 CFR

2010-07-01

... dangerous cargo. 6.12-3 Section 6.12-3 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... Control of Explosives or Other Dangerous Cargo § 6.12-3 Approval of facility for dangerous cargo. The... discharging, explosives, inflammable or combustible liquids in bulk, or other dangerous articles or cargo...

33 CFR 401.80 - Reporting dangerous cargo.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Reporting dangerous cargo. 401.80... dangerous cargo. (a) The master of any explosive vessel or hazardous cargo vessel shall report to a Seaway station, as set out in Schedule III, the nature, quantity, and IMO classification of the dangerous cargo...

33 CFR 401.80 - Reporting dangerous cargo.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Reporting dangerous cargo. 401.80... dangerous cargo. (a) The master of any explosive vessel or hazardous cargo vessel shall report to a Seaway station, as set out in Schedule III, the nature, quantity, and IMO classification of the dangerous cargo...

33 CFR 6.12-3 - Approval of facility for dangerous cargo.

Code of Federal Regulations, 2013 CFR

2013-07-01

... dangerous cargo. 6.12-3 Section 6.12-3 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... Control of Explosives or Other Dangerous Cargo § 6.12-3 Approval of facility for dangerous cargo. The... discharging, explosives, inflammable or combustible liquids in bulk, or other dangerous articles or cargo...

33 CFR 401.80 - Reporting dangerous cargo.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Reporting dangerous cargo. 401.80... dangerous cargo. (a) The master of any explosive vessel or hazardous cargo vessel shall report to a Seaway station, as set out in Schedule III, the nature, quantity, and IMO classification of the dangerous cargo...

33 CFR 401.80 - Reporting dangerous cargo.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Reporting dangerous cargo. 401.80... dangerous cargo. (a) The master of any explosive vessel or hazardous cargo vessel shall report to a Seaway station, as set out in Schedule III, the nature, quantity, and IMO classification of the dangerous cargo...

33 CFR 6.12-3 - Approval of facility for dangerous cargo.

Code of Federal Regulations, 2011 CFR

2011-07-01

... dangerous cargo. 6.12-3 Section 6.12-3 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... Control of Explosives or Other Dangerous Cargo § 6.12-3 Approval of facility for dangerous cargo. The... discharging, explosives, inflammable or combustible liquids in bulk, or other dangerous articles or cargo...

33 CFR 6.12-3 - Approval of facility for dangerous cargo.

Code of Federal Regulations, 2014 CFR

2014-07-01

... dangerous cargo. 6.12-3 Section 6.12-3 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... Control of Explosives or Other Dangerous Cargo § 6.12-3 Approval of facility for dangerous cargo. The... discharging, explosives, inflammable or combustible liquids in bulk, or other dangerous articles or cargo...

33 CFR 6.12-3 - Approval of facility for dangerous cargo.

Code of Federal Regulations, 2012 CFR

2012-07-01

... dangerous cargo. 6.12-3 Section 6.12-3 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... Control of Explosives or Other Dangerous Cargo § 6.12-3 Approval of facility for dangerous cargo. The... discharging, explosives, inflammable or combustible liquids in bulk, or other dangerous articles or cargo...

33 CFR 401.80 - Reporting dangerous cargo.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Reporting dangerous cargo. 401.80... dangerous cargo. (a) The master of any explosive vessel or hazardous cargo vessel shall report to a Seaway station, as set out in Schedule III, the nature, quantity, and IMO classification of the dangerous cargo...

46 CFR 153.251 - Independent cargo tanks.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Independent cargo tanks. 153.251 Section 153.251... CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Tanks § 153.251 Independent cargo tanks. All independent cargo tank must meet § 38.05-10 (a)(1), (b), (d), and...

46 CFR 153.251 - Independent cargo tanks.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Independent cargo tanks. 153.251 Section 153.251... CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Tanks § 153.251 Independent cargo tanks. All independent cargo tank must meet § 38.05-10 (a)(1), (b), (d), and...

46 CFR 153.436 - Heat transfer fluids: compatibility with cargo.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Heat transfer fluids: compatibility with cargo. 153.436... Equipment Cargo Temperature Control Systems § 153.436 Heat transfer fluids: compatibility with cargo. A heat transfer fluid separated from the cargo by only one wall (for example, the heat transfer fluid in a coil...

46 CFR 153.436 - Heat transfer fluids: compatibility with cargo.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Heat transfer fluids: compatibility with cargo. 153.436... Equipment Cargo Temperature Control Systems § 153.436 Heat transfer fluids: compatibility with cargo. A heat transfer fluid separated from the cargo by only one wall (for example, the heat transfer fluid in a coil...

46 CFR 153.436 - Heat transfer fluids: compatibility with cargo.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Heat transfer fluids: compatibility with cargo. 153.436... Equipment Cargo Temperature Control Systems § 153.436 Heat transfer fluids: compatibility with cargo. A heat transfer fluid separated from the cargo by only one wall (for example, the heat transfer fluid in a coil...

46 CFR 154.901 - Atmospheric control within cargo tanks and cargo piping systems.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Atmospheric control within cargo tanks and cargo piping systems. 154.901 Section 154.901 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN..., Construction and Equipment Atmospheric Control in Cargo Containment Systems § 154.901 Atmospheric control...

46 CFR 154.901 - Atmospheric control within cargo tanks and cargo piping systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Atmospheric control within cargo tanks and cargo piping systems. 154.901 Section 154.901 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN..., Construction and Equipment Atmospheric Control in Cargo Containment Systems § 154.901 Atmospheric control...

46 CFR 154.901 - Atmospheric control within cargo tanks and cargo piping systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Atmospheric control within cargo tanks and cargo piping systems. 154.901 Section 154.901 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN..., Construction and Equipment Atmospheric Control in Cargo Containment Systems § 154.901 Atmospheric control...

46 CFR 154.901 - Atmospheric control within cargo tanks and cargo piping systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Atmospheric control within cargo tanks and cargo piping systems. 154.901 Section 154.901 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN..., Construction and Equipment Atmospheric Control in Cargo Containment Systems § 154.901 Atmospheric control...

46 CFR 154.901 - Atmospheric control within cargo tanks and cargo piping systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Atmospheric control within cargo tanks and cargo piping systems. 154.901 Section 154.901 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN..., Construction and Equipment Atmospheric Control in Cargo Containment Systems § 154.901 Atmospheric control...

46 CFR 153.333 - Cargo pump discharge pressure gauge.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pump discharge pressure gauge. 153.333 Section 153... SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Pumprooms § 153.333 Cargo pump discharge pressure gauge. Each cargo pump within a pump-room must...

46 CFR 153.336 - Special cargo pump or pumproom requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Special cargo pump or pumproom requirements. 153.336... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Pumprooms § 153.336 Special cargo pump or pumproom requirements. (a) When Table 1 refers to...

46 CFR 154.412 - Cargo tank corrosion allowance.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Cargo tank corrosion allowance. 154.412 Section 154.412... Containment Systems § 154.412 Cargo tank corrosion allowance. A cargo tank must be designed with a corrosion...) carries a cargo that corrodes the tank material. Note: Corrosion allowance for independent tank type C is...

46 CFR 154.412 - Cargo tank corrosion allowance.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank corrosion allowance. 154.412 Section 154.412... Containment Systems § 154.412 Cargo tank corrosion allowance. A cargo tank must be designed with a corrosion...) carries a cargo that corrodes the tank material. Note: Corrosion allowance for independent tank type C is...

46 CFR 153.333 - Cargo pump discharge pressure gauge.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Cargo pump discharge pressure gauge. 153.333 Section 153... SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Pumprooms § 153.333 Cargo pump discharge pressure gauge. Each cargo pump within a pump-room must...

46 CFR 153.333 - Cargo pump discharge pressure gauge.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Cargo pump discharge pressure gauge. 153.333 Section 153... SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Pumprooms § 153.333 Cargo pump discharge pressure gauge. Each cargo pump within a pump-room must...

46 CFR 153.333 - Cargo pump discharge pressure gauge.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Cargo pump discharge pressure gauge. 153.333 Section 153... SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Pumprooms § 153.333 Cargo pump discharge pressure gauge. Each cargo pump within a pump-room must...

46 CFR 153.333 - Cargo pump discharge pressure gauge.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Cargo pump discharge pressure gauge. 153.333 Section 153... SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Pumprooms § 153.333 Cargo pump discharge pressure gauge. Each cargo pump within a pump-room must...

49 CFR 176.720 - Requirements for carriage of INF cargo in international transportation.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 2 2014-10-01 2014-10-01 false Requirements for carriage of INF cargo in... Requirements for carriage of INF cargo in international transportation. In addition to all other applicable requirements of this subchapter, a vessel carrying INF cargo (see § 176.2, under INF cargo definition) in...

49 CFR 176.720 - Requirements for carriage of INF cargo in international transportation.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 2 2012-10-01 2012-10-01 false Requirements for carriage of INF cargo in... Requirements for carriage of INF cargo in international transportation. In addition to all other applicable requirements of this subchapter, a vessel carrying INF cargo (see § 176.2, under INF cargo definition) in...

49 CFR 176.720 - Requirements for carriage of INF cargo in international transportation.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 2 2013-10-01 2013-10-01 false Requirements for carriage of INF cargo in... Requirements for carriage of INF cargo in international transportation. In addition to all other applicable requirements of this subchapter, a vessel carrying INF cargo (see § 176.2, under INF cargo definition) in...

49 CFR 176.720 - Requirements for carriage of INF cargo in international transportation.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Requirements for carriage of INF cargo in... Requirements for carriage of INF cargo in international transportation. In addition to all other applicable requirements of this subchapter, a vessel carrying INF cargo (see § 176.2, under INF cargo definition) in...

49 CFR 176.720 - Requirements for carriage of INF cargo in international transportation.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 2 2011-10-01 2011-10-01 false Requirements for carriage of INF cargo in... Requirements for carriage of INF cargo in international transportation. In addition to all other applicable requirements of this subchapter, a vessel carrying INF cargo (see § 176.2, under INF cargo definition) in...

46 CFR 154.1828 - Spaces containing cargo vapor: Entry.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Spaces containing cargo vapor: Entry. 154.1828 Section... Spaces containing cargo vapor: Entry. (a) No person may enter a cargo handling space without the... allowing anyone to enter a cargo handling space, the master shall ensure that: (1) The space is free of...

46 CFR 154.1828 - Spaces containing cargo vapor: Entry.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Spaces containing cargo vapor: Entry. 154.1828 Section... Spaces containing cargo vapor: Entry. (a) No person may enter a cargo handling space without the... allowing anyone to enter a cargo handling space, the master shall ensure that: (1) The space is free of...

46 CFR 154.1828 - Spaces containing cargo vapor: Entry.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Spaces containing cargo vapor: Entry. 154.1828 Section... Spaces containing cargo vapor: Entry. (a) No person may enter a cargo handling space without the... allowing anyone to enter a cargo handling space, the master shall ensure that: (1) The space is free of...

19 CFR 122.117 - Requirements for transit air cargo transport.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 19 Customs Duties 1 2010-04-01 2010-04-01 false Requirements for transit air cargo transport. 122... Requirements for transit air cargo transport. (a) Transportation—(1) Port to port. Transit air cargo may be... cargo, a receipt shall be given. The receipt shall be made by the airline responsible for transport or...

19 CFR 122.117 - Requirements for transit air cargo transport.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 19 Customs Duties 1 2011-04-01 2011-04-01 false Requirements for transit air cargo transport. 122... Requirements for transit air cargo transport. (a) Transportation—(1) Port to port. Transit air cargo may be... cargo, a receipt shall be given. The receipt shall be made by the airline responsible for transport or...

46 CFR 153.934 - Entry into spaces containing cargo vapor.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Entry into spaces containing cargo vapor. 153.934... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations General Vessel Safety § 153.934 Entry into spaces containing cargo vapor. (a) No person may enter a cargo...

46 CFR 153.934 - Entry into spaces containing cargo vapor.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Entry into spaces containing cargo vapor. 153.934... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations General Vessel Safety § 153.934 Entry into spaces containing cargo vapor. (a) No person may enter a cargo...

46 CFR 153.934 - Entry into spaces containing cargo vapor.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Entry into spaces containing cargo vapor. 153.934... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations General Vessel Safety § 153.934 Entry into spaces containing cargo vapor. (a) No person may enter a cargo...

46 CFR 153.934 - Entry into spaces containing cargo vapor.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Entry into spaces containing cargo vapor. 153.934... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations General Vessel Safety § 153.934 Entry into spaces containing cargo vapor. (a) No person may enter a cargo...

46 CFR 153.934 - Entry into spaces containing cargo vapor.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Entry into spaces containing cargo vapor. 153.934... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations General Vessel Safety § 153.934 Entry into spaces containing cargo vapor. (a) No person may enter a cargo...

46 CFR 153.436 - Heat transfer fluids: compatibility with cargo.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Heat transfer fluids: compatibility with cargo. 153.436... Equipment Cargo Temperature Control Systems § 153.436 Heat transfer fluids: compatibility with cargo. A heat transfer fluid separated from the cargo by only one wall (for example, the heat transfer fluid in a coil...

46 CFR 153.436 - Heat transfer fluids: compatibility with cargo.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Heat transfer fluids: compatibility with cargo. 153.436... Equipment Cargo Temperature Control Systems § 153.436 Heat transfer fluids: compatibility with cargo. A heat transfer fluid separated from the cargo by only one wall (for example, the heat transfer fluid in a coil...

46 CFR 308.521 - Application for Open Cargo Policy, Form MA-301.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Section 308.521 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.521 Application for Open Cargo Policy, Form MA-301. The standard form of application for a War Risk Open Cargo Policy...

46 CFR 308.517 - Open Cargo Policy, Form MA-300.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Open Policy War Risk Cargo Insurance § 308.517 Open Cargo Policy, Form MA-300. The standard form of War Risk Open Cargo, Form MA-300, may be obtained from MARAD's underwriting agent or MARAD. ...

46 CFR 308.521 - Application for Open Cargo Policy, Form MA-301.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Section 308.521 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.521 Application for Open Cargo Policy, Form MA-301. The standard form of application for a War Risk Open Cargo Policy...

46 CFR 308.517 - Open Cargo Policy, Form MA-300.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.517 Open Cargo Policy, Form MA-300. The standard form of War Risk Open Cargo, Form MA-300, may be obtained from the American War Risk...

46 CFR 308.521 - Application for Open Cargo Policy, Form MA-301.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Section 308.521 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.521 Application for Open Cargo Policy, Form MA-301. The standard form of application for a War Risk Open Cargo Policy...

46 CFR 308.517 - Open Cargo Policy, Form MA-300.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.517 Open Cargo Policy, Form MA-300. The standard form of War Risk Open Cargo, Form MA-300, may be obtained from the American War Risk...

46 CFR 308.508 - Issuance of an Open Cargo Policy.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Issuance of an Open Cargo Policy. 308.508 Section 308.508 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Open Policy War Risk Cargo Insurance § 308.508 Issuance of an Open Cargo...

46 CFR 308.517 - Open Cargo Policy, Form MA-300.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.517 Open Cargo Policy, Form MA-300. The standard form of War Risk Open Cargo, Form MA-300, may be obtained from the American War Risk...

46 CFR 308.517 - Open Cargo Policy, Form MA-300.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.517 Open Cargo Policy, Form MA-300. The standard form of War Risk Open Cargo, Form MA-300, may be obtained from the American War Risk...

46 CFR 308.521 - Application for Open Cargo Policy, Form MA-301.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Section 308.521 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Open Policy War Risk Cargo Insurance § 308.521 Application for Open Cargo Policy, Form MA-301. The standard form of application for a War Risk Open Cargo Policy may...

46 CFR 308.521 - Application for Open Cargo Policy, Form MA-301.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Section 308.521 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.521 Application for Open Cargo Policy, Form MA-301. The standard form of application for a War Risk Open Cargo Policy...

Selectivity Mechanism of the Nuclear Pore Complex Characterized by Single Cargo Tracking

PubMed Central

Lowe, Alan R.; Siegel, Jake J.; Kalab, Petr; Siu, Merek; Weis, Karsten; Liphardt, Jan T.

2010-01-01

The Nuclear Pore Complex (NPC) mediates all exchange between the cytoplasm and the nucleus. Small molecules can passively diffuse through the NPC, while larger cargos require transport receptors to translocate1. How the NPC facilitates the translocation of transport receptor/cargo complexes remains unclear. Here, we track single protein-functionalized Quantum Dot (QD) cargos as they translocate the NPC. Import proceeds by successive sub-steps comprising cargo capture, filtering and translocation, and release into the nucleus. The majority of QDs are rejected at one of these steps and return to the cytoplasm including very large cargos that abort at a size-selective barrier. Cargo movement in the central channel is subdiffusive and cargos that can bind more transport receptors diffuse more freely. Without Ran, cargos still explore the entire NPC, but have a markedly reduced probability of exit into the nucleus, suggesting that NPC entry and exit steps are not equivalent and that the pore is functionally asymmetric to importing cargos. The overall selectivity of the NPC appears to arise from the cumulative action of multiple reversible sub-steps and a final irreversible exit step. PMID:20811366

Safety recommendations and responses availability. [Training of shipmasters in automated radar plotting

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1980-09-25

The U.S. National Transportation Safety Board (NTSB) has recommended that the U.S. Coast Guard (USCG) and Exxon Co. USA require shipmasters assigned to a vessel equipped with an automated radar plotting aid to complete an approved training course before assignment to avoid the recurrence of accidents similar to the collision of the Exxon Chester tankship and the Regal Sword Liberian freighter in the Atlantic Ocean near Cape Cod, Massachusetts, on 6/18/79. Following the explosion, burning, and sinking of the Liberian tankship, Seatiger, at a Sun Oil Terminal Inc. berth in Nederland, Texas on 4/19/79, NTSB also urged USCG to requiremore » foreign and domestic crude oil tankers of 20,000 dwt and above that are equipped with inert systems to place these systems in operation while in US waters, except when cargo tanks are gas-free; instructed Sun Oil Terminals Inc. to provide an emergency communication system between berthed vessels and its terminal office during communication system between berthed vessels and its terminal office during cargo discharging; and recommended that the American Bureau of Shipping instruct tanker surveyors to examine flame screens. Other safety recommendations and responses by federal agencies are also discussed.« less

Scaling beta-delayed neutron measurements to large detector areas

NASA Astrophysics Data System (ADS)

Sutanto, F.; Nattress, J.; Jovanovic, I.

2017-08-01

We explore the performance of a cargo screening system that consists of two large-sized composite scintillation detectors and a high-energy neutron interrogation source by modeling and simulation. The goal of the system is to measure β-delayed neutron emission from an illicit special nuclear material by use of active interrogation. This task is challenging because the β-delayed neutron yield is small in comparison with the yield of the prompt fission secondary products, β-delayed neutrons are emitted with relatively low energies, and high neutron and gamma backgrounds are typically present. Detectors used to measure delayed neutron emission must exhibit high intrinsic efficiency and cover a large solid angle, which also makes them sensitive to background neutron radiation. We present a case study where we attempt to detect the presence of 5 kg-scale quantities of 235U in a standard air-filled cargo container using 14 MeV neutrons as a probe. We find that by using a total measurement time of ˜11.6 s and a dose equivalent of ˜1.7 mrem, the presence of 235U can be detected with false positive and false negative probabilities that are both no larger than 0.1%.

An outlook for cargo aircraft of the future. [assessment of the future of air cargo by analyzing statistics and trends

NASA Technical Reports Server (NTRS)

Nicks, O. W.; Whitehead, A. H., Jr.; Alford, W. J., Jr.

1975-01-01

An assessment is provided of the future of air cargo by analyzing air cargo statistics and trends, by noting air cargo system problems and inefficiencies, by analyzing characteristics of air-eligible commodities, and by showing the promise of new technology for future cargo aircraft with significant improvements in costs and efficiency. NASA's proposed program is reviewed which would sponsor the research needed to provide for development of advanced designs by 1985.

Length of encapsidated cargo impacts stability and structure of in vitro assembled alphavirus core-like particles

NASA Astrophysics Data System (ADS)

Rayaprolu, Vamseedhar; Moore, Alan; Che-Yen Wang, Joseph; Goh, Boon Chong; Perilla, Juan R.; Zlotnick, Adam; Mukhopadhyay, Suchetana

2017-12-01

In vitro assembly of alphavirus nucleocapsid cores, called core-like particles (CLPs), requires a polyanionic cargo. There are no sequence or structure requirements to encapsidate single-stranded nucleic acid cargo. In this work, we wanted to determine how the length of the cargo impacts the stability and structure of the assembled CLPs. We hypothesized that cargo neutralizes the basic region of the alphavirus capsid protein and if the cargo is long enough, it will also act to scaffold the CP monomers together. Experimentally we found that CLPs encapsidating short 27mer oligonucleotides were less stable than CLPs encapsidating 48mer or 90mer oligonucleotides under different chemical and thermal conditions. Furthermore, cryo-EM studies showed there were structural differences between CLPs assembled with 27mer and 48mer cargo. To mimic the role of the cargo in CLP assembly we made a mutant (4D) where we substituted a cluster of four Lys residues in the CP with four Asp residues. We found that these few amino acid substitutions were enough to initiate CLP assembly in the absence of cargo. The cargo-free 4D CLPs show higher resistance to ionic strength and increased temperature compared to wild-type cargo containing CLPs suggesting their CLP assembly mechanism might also be different.

Non-destructive automated sampling of mycotoxins in bulk food and feed - A new tool for required harmonization.

PubMed

Spanjer, M; Stroka, J; Patel, S; Buechler, S; Pittet, A; Barel, S

2001-06-01

Mycotoxins contamination is highly non-uniformly distributed as is well recog-nized by the EC, by not only setting legal limits in a series of commodities, but also schedule a sampling plan that takes this heterogeneity into account. In practice however, it turns out that it is very difficult to carry out this sampling plan in a harmonised way. Applying the sampling plan to a container filled with pallets of bags (i.e. with nuts or coffee beans) varies from very laborious to almost impossible. The presented non-destructive automated method to sample bulk food could help to overcome these practical problems and to enforcing of EC directives. It is derived from a tested and approved technology for detection of illicit substances in security applications. It has capability to collect and iden-tify ultra trace contaminants, i.e. from a fingerprint of chemical substance in a bulk of goods, a cargo pallet load (~ 1000 kg) with boxes and commodities.The technology, patented for explosives detection, uses physical and chemistry processes for excitation and remote rapid enhanced release of contaminant residues, vapours and particulate, of the inner/outer surfaces of inspected bulk and collect them on selective probes. The process is automated, takes only 10 minutes, is non-destructive and the bulk itself remains unharmed. The system design is based on applicable international regulations for shipped cargo hand-ling and transportation by road, sea and air. After this process the pallet can be loaded on a truck, ship or plane. Analysis can be carried out before the cargo leaves the place of shipping. The potent application of this technology for myco-toxins detection, has been demonstrated by preliminary feasibility experiments. Aflatoxins were detected in pistachios and ochratoxin A in green coffee beans bulk. Both commodities were naturally contaminated, priory found and confirm-ed by common methods as used at routine inspections. Once the contaminants are extracted from a bulk shipment, an appropriate existing analytical method, i.e. a CEN method, can be used to measure the mycotoxins.The system, routinely in use for explosives detection, was able to screen bulk food and feed for mycotoxins, through non-destructive automated sampling of a whole batch/lot/sublot of commodities. The opportunity to sample a whole bulk would provide more effective tools for inspection at seaports, production facili-ties and distri-bution points. It will advance the current process of myco-toxins check because: (i) Checks will be automated and harmonized, (ii) Checks will be non-destructive, (iii) Checks will be faster and allow a greater amount of bulk commodities to be inspected and (iv) The ability to check, with automated equipment, larger portions of lots of a shipment will increase the probability to detect the heterogeneous mycotoxins contamination in bulk foods. The poster provides some results of feasibility experiments indicating the capability of this technology for inspection of commodities bulks for the detection of mycotoxins, at legal limits, in naturally contaminated food.

46 CFR 150.160 - Carrying a cargo as an exception to the compatibility chart.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Carrying a cargo as an exception to the compatibility chart. 150.160 Section 150.160 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.160 Carrying a cargo as an exception to the...

46 CFR 150.160 - Carrying a cargo as an exception to the compatibility chart.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Carrying a cargo as an exception to the compatibility chart. 150.160 Section 150.160 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.160 Carrying a cargo as an exception to the...

46 CFR 150.160 - Carrying a cargo as an exception to the compatibility chart.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Carrying a cargo as an exception to the compatibility chart. 150.160 Section 150.160 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.160 Carrying a cargo as an exception to the...

46 CFR 150.160 - Carrying a cargo as an exception to the compatibility chart.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Carrying a cargo as an exception to the compatibility chart. 150.160 Section 150.160 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.160 Carrying a cargo as an exception to the...

46 CFR 150.160 - Carrying a cargo as an exception to the compatibility chart.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Carrying a cargo as an exception to the compatibility chart. 150.160 Section 150.160 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.160 Carrying a cargo as an exception to the...

Cargo/Logistics Airlift System Study (CLASS), Executive Summary

NASA Technical Reports Server (NTRS)

Norman, J. M.; Henderson, R. D.; Macey, F. C.; Tuttle, R. P.

1978-01-01

The current air cargo system is analyzed along with advanced air cargo systems studies. A forecast of advanced air cargo system demand is presented with cost estimates. It is concluded that there is a need for a dedicated advance air cargo system, and with application of advanced technology, reductions of 45% in air freight rates may be achieved.

46 CFR 153.297 - Emergency actuators at the point of cargo control.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Emergency actuators at the point of cargo control. 153... and Equipment Piping Systems and Cargo Handling Equipment § 153.297 Emergency actuators at the point of cargo control. (a) The point from which cargo transfer is controlled must have the same actuators...

46 CFR 153.935a - Storage of cargo samples.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Storage of cargo samples. 153.935a Section 153.935a Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... § 153.935a Storage of cargo samples. (a) The master shall make sure that any cargo samples are stored in...

46 CFR 36.10-1 - Cargo pump relief valves-TB/ALL.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo pump relief valves-TB/ALL. 36.10-1 Section 36.10-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS ELEVATED TEMPERATURE CARGOES Piping, Valves, Fittings, and Accessory Equipment § 36.10-1 Cargo pump relief valves—TB/ALL. (a) Cargo pump...

14 CFR 121.583 - Carriage of persons without compliance with the passenger-carrying requirements of this part.

Code of Federal Regulations, 2010 CFR

2010-01-01

... is governed by regulations in 49 CFR part 175; (iv) The security of valuable or confidential cargo; (v) The preservation of fragile or perishable cargo; (vi) Experiments on, or testing of, cargo containers or cargo handling devices; (vii) The operation of special equipment for loading or unloading cargo...

14 CFR 121.583 - Carriage of persons without compliance with the passenger-carrying requirements of this part.

Code of Federal Regulations, 2012 CFR

2012-01-01

... is governed by regulations in 49 CFR part 175; (iv) The security of valuable or confidential cargo; (v) The preservation of fragile or perishable cargo; (vi) Experiments on, or testing of, cargo containers or cargo handling devices; (vii) The operation of special equipment for loading or unloading cargo...

14 CFR 121.583 - Carriage of persons without compliance with the passenger-carrying requirements of this part.

Code of Federal Regulations, 2011 CFR

2011-01-01

... is governed by regulations in 49 CFR part 175; (iv) The security of valuable or confidential cargo; (v) The preservation of fragile or perishable cargo; (vi) Experiments on, or testing of, cargo containers or cargo handling devices; (vii) The operation of special equipment for loading or unloading cargo...

46 CFR 32.50-30 - Cargo hose-TB/ALL.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Cargo hose-TB/ALL. 32.50-30 Section 32.50-30 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Pumps, Piping, and Hose for Cargo Handling § 32.50-30 Cargo hose—TB/ALL. Cargo hose carried on...

46 CFR 32.50-30 - Cargo hose-TB/ALL.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Cargo hose-TB/ALL. 32.50-30 Section 32.50-30 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Pumps, Piping, and Hose for Cargo Handling § 32.50-30 Cargo hose—TB/ALL. Cargo hose carried on...

46 CFR 30.10-6 - Cargo handling room-TB/ALL.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Cargo handling room-TB/ALL. 30.10-6 Section 30.10-6 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS GENERAL PROVISIONS Definitions § 30.10-6 Cargo handling room—TB/ALL. The term cargo handling room means any enclosed space where cargo is pumped...

46 CFR 30.10-6 - Cargo handling room-TB/ALL.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo handling room-TB/ALL. 30.10-6 Section 30.10-6 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS GENERAL PROVISIONS Definitions § 30.10-6 Cargo handling room—TB/ALL. The term cargo handling room means any enclosed space where cargo is pumped...

46 CFR 32.50-30 - Cargo hose-TB/ALL.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo hose-TB/ALL. 32.50-30 Section 32.50-30 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Pumps, Piping, and Hose for Cargo Handling § 32.50-30 Cargo hose—TB/ALL. Cargo hose carried on...

46 CFR 30.10-6 - Cargo handling room-TB/ALL.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Cargo handling room-TB/ALL. 30.10-6 Section 30.10-6 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS GENERAL PROVISIONS Definitions § 30.10-6 Cargo handling room—TB/ALL. The term cargo handling room means any enclosed space where cargo is pumped...

46 CFR 30.10-6 - Cargo handling room-TB/ALL.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Cargo handling room-TB/ALL. 30.10-6 Section 30.10-6 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS GENERAL PROVISIONS Definitions § 30.10-6 Cargo handling room—TB/ALL. The term cargo handling room means any enclosed space where cargo is pumped...