Science.gov

Sample records for celso bianco md

  1. Stratigraphy and depositional history of the Pliocene Bianco section, Calabria, southern Italy

    USGS Publications Warehouse

    Rio, D.; Thunell, R.; Sprovieri, R.; Bukry, D.; Destefano, E.; Howell, M.; Raffi, I.; Sancetta, C.; Sanfilippo, A.

    1989-01-01

    An integrated micropaleontological and geochemical study was carried out on the Pliocene-age Bianco section located in Calabria, southern Italy. This section is somewhat unique for the Pliocene of the Mediterranean region in that it contains abundant calcareous and siliceous microfossils. Based on the biostratigraphic findings, it ranges in age from approximately 3.7-3.0 Ma. The Bianco section is composed of marly mudstones intercalated with diatomites, with the diatomites being particularly common in the upper 50 m of the section (above 3.1 Ma). The diatomites contain an abundant benthic foraminiferal assemblage and have a low organic carbon content indicating that bottom waters were fairly well-oxygenated during their deposition. Faunal and floral indicators suggest a cooling of surface waters in this region at 3.1 Ma. The diatom assemblages within the Bianco diatomites are very similar to those living in the Gulf of California, suggesting an upwelling origin for these silica-rich units. A model is proposed which attributes diatomite formation to upwelling induced by climatically controlled changes in local hydrography. ?? 1989.

  2. Developmental, transcriptome, and genetic alterations associated with parthenocarpy in the grapevine seedless somatic variant Corinto bianco.

    PubMed

    Royo, Carolina; Carbonell-Bejerano, Pablo; Torres-Pérez, Rafael; Nebish, Anna; Martínez, Óscar; Rey, Manuel; Aroutiounian, Rouben; Ibáñez, Javier; Martínez-Zapater, José M

    2016-01-01

    Seedlessness is a relevant trait in grapevine cultivars intended for fresh consumption or raisin production. Previous DNA marker analysis indicated that Corinto bianco (CB) is a parthenocarpic somatic variant of the seeded cultivar Pedro Ximenes (PX). This study compared both variant lines to determine the basis of this parthenocarpic phenotype. At maturity, CB seedless berries were 6-fold smaller than PX berries. The macrogametophyte was absent from CB ovules, and CB was also pollen sterile. Occasionally, one seed developed in 1.6% of CB berries. Microsatellite genotyping and flow cytometry analyses of seedlings generated from these seeds showed that most CB viable seeds were formed by fertilization of unreduced gametes generated by meiotic diplospory, a process that has not been described previously in grapevine. Microarray and RNA-sequencing analyses identified 1958 genes that were differentially expressed between CB and PX developing flowers. Genes downregulated in CB were enriched in gametophyte-preferentially expressed transcripts, indicating the absence of regular post-meiotic germline development in CB. RNA-sequencing was also used for genetic variant calling and 14 single-nucleotide polymorphisms distinguishing the CB and PX variant lines were detected. Among these, CB-specific polymorphisms were considered as candidate parthenocarpy-responsible mutations, including a putative deleterious substitution in a HAL2-like protein. Collectively, these results revealed that the absence of a mature macrogametophyte, probably due to meiosis arrest, coupled with a process of fertilization-independent fruit growth, caused parthenocarpy in CB. This study provides a number of grapevine parthenocarpy-responsible candidate genes and shows how genomic approaches can shed light on the genetic origin of woody crop somatic variants. PMID:26454283

  3. Developmental, transcriptome, and genetic alterations associated with parthenocarpy in the grapevine seedless somatic variant Corinto bianco.

    PubMed

    Royo, Carolina; Carbonell-Bejerano, Pablo; Torres-Pérez, Rafael; Nebish, Anna; Martínez, Óscar; Rey, Manuel; Aroutiounian, Rouben; Ibáñez, Javier; Martínez-Zapater, José M

    2016-01-01

    Seedlessness is a relevant trait in grapevine cultivars intended for fresh consumption or raisin production. Previous DNA marker analysis indicated that Corinto bianco (CB) is a parthenocarpic somatic variant of the seeded cultivar Pedro Ximenes (PX). This study compared both variant lines to determine the basis of this parthenocarpic phenotype. At maturity, CB seedless berries were 6-fold smaller than PX berries. The macrogametophyte was absent from CB ovules, and CB was also pollen sterile. Occasionally, one seed developed in 1.6% of CB berries. Microsatellite genotyping and flow cytometry analyses of seedlings generated from these seeds showed that most CB viable seeds were formed by fertilization of unreduced gametes generated by meiotic diplospory, a process that has not been described previously in grapevine. Microarray and RNA-sequencing analyses identified 1958 genes that were differentially expressed between CB and PX developing flowers. Genes downregulated in CB were enriched in gametophyte-preferentially expressed transcripts, indicating the absence of regular post-meiotic germline development in CB. RNA-sequencing was also used for genetic variant calling and 14 single-nucleotide polymorphisms distinguishing the CB and PX variant lines were detected. Among these, CB-specific polymorphisms were considered as candidate parthenocarpy-responsible mutations, including a putative deleterious substitution in a HAL2-like protein. Collectively, these results revealed that the absence of a mature macrogametophyte, probably due to meiosis arrest, coupled with a process of fertilization-independent fruit growth, caused parthenocarpy in CB. This study provides a number of grapevine parthenocarpy-responsible candidate genes and shows how genomic approaches can shed light on the genetic origin of woody crop somatic variants.

  4. Creativity and innovation by empowering the customer: The case of Mulino Bianco. Part II: The digital customer value added

    NASA Astrophysics Data System (ADS)

    Bujor, A.; Avasilcăi, S.

    2015-11-01

    The terms of creativity, co-creation, creative industries, innovation, and coinnovation are more and more used nowadays. While co-creation offers the possibility and encourages a more active involvement from the customers to create value rich experiences, innovation is responsible for the little improvements made for a better life, to grow a business, to improve products, services or company's productivity. Either customers, current and potential, or stakeholders’ involvement into innovation activities, through their creativity, represent an important way of value creation, of actions’ performance that increases the worth of goods, services, or business as a whole. More and more, different size businesses gather ideas for innovation from customers / stakeholders by involving them into the early stages of the innovation process. Actually, it has been shown that their ideas sketch their needs and wishes, and have been described as “need information”. Customers and stakeholders, in general, also offer ideas that have been called “solution information”, which represents, not only need information, but also customer-based proposals that describe how ideas can be transformed into marketable products. The term of creative industries refers to those goods that can technically be reproduced, industrially produced, and commercially sold, this being one of the many definitions found in the literature. Mulino Bianco was first launched in Italy, being one of the value brands of Barilla Group, which we can say, according to the Italian definition and classification, it belongs to creative industries: industry of food and taste. Even though Barilla Group's Research & Development department does its job very efficiently, developing and creating new products under different brands, lately a key strategy for the Group and for Mulino Bianco, by default, is customers’ pro-active involvement in products’ development or creation. One of the tools used for this is the

  5. Creativity and innovation by empowering the customer: The case of Mulino Bianco. Part I: Organisational innovation context

    NASA Astrophysics Data System (ADS)

    Bujor, A.; Avasilcăi, S.

    2015-11-01

    The terms of creativity, co-creation, creative industries, innovation, and coinnovation are more and more used nowadays. While co-creation offers the possibility and encourages a more active involvement from the customers to create value rich experiences, innovation is responsible for the little improvements made for a better life, to grow a business, to improve products, services or company's productivity. Either customers, current and potential, or stakeholders’ involvement into innovation activities, through their creativity, represent an important way of value creation, of actions’ performance that increases the worth of goods, services, or business as a whole. More and more, different size businesses gather ideas for innovation from customers / stakeholders through their involvement into the early stages of the innovation process. Actually, it has been shown that their ideas sketch their needs and wishes, and have been described as “need information”. Customers and stakeholders, in general, also offer ideas that have been called “solution information”, which represents, not only need information, but also customer-based proposals that describe how ideas can be transformed into marketable products. The term of creative industries refers to those goods that can technically be reproduced, industrially produced, and commercially sold, this being one of the many definitions found in the literature. The classification of creative industries differs at the European Union level according to each country's development level and opening towards these industries’ perception as an engine for economic growth. To better explore and identify the importance of innovation and creativity for new products’ development or improvement a case study about Mulino Bianco was done. This methodology approach represents a part of a qualitative research approach within a broader research undertaken within the framework of the National Research Program PN II. Mulino Bianco

  6. MD-2 binds cholesterol.

    PubMed

    Choi, Soo-Ho; Kim, Jungsu; Gonen, Ayelet; Viriyakosol, Suganya; Miller, Yury I

    2016-02-19

    Cholesterol is a structural component of cellular membranes, which is transported from liver to peripheral cells in the form of cholesterol esters (CE), residing in the hydrophobic core of low-density lipoprotein. Oxidized CE (OxCE) is often found in plasma and in atherosclerotic lesions of subjects with cardiovascular disease. Our earlier studies have demonstrated that OxCE activates inflammatory responses in macrophages via toll-like receptor-4 (TLR4). Here we demonstrate that cholesterol binds to myeloid differentiation-2 (MD-2), a TLR4 ancillary molecule, which is a binding receptor for bacterial lipopolysaccharide (LPS) and is indispensable for LPS-induced TLR4 dimerization and signaling. Cholesterol binding to MD-2 was competed by LPS and by OxCE-modified BSA. Furthermore, soluble MD-2 in human plasma and MD-2 in mouse atherosclerotic lesions carried cholesterol, the finding supporting the biological significance of MD-2 cholesterol binding. These results help understand the molecular basis of TLR4 activation by OxCE and mechanisms of chronic inflammation in atherosclerosis.

  7. Arnold Aberman, MD.

    PubMed

    Aberman, A

    1995-10-01

    When the United States embarked on its effort to provide universal health insurance, the Canadian Medicare System was cited as a possible model for American health care. Often touted as an example of low-cost, high quality medicine, the Canadian system has mirrored the problems of health care across its southern border. With rocketing health care expenditures and financing having largely been decentralized to the individual provinces, local officials have struggled to cut costs and services. A central focus of these efforts has been a move to decrease the numbers of physicians, most notably a 10% decrease in medical school class size in 1993. While some Western provinces have experimented with the privatization of health care, the Canadian system still remains the epitome of government operated fee-for-service medicine. Given the likelihood of dramatic change in the American Medicare system, Canadian academic centers offer a unique perspective on the impact of capitation, evolving relationships with government payors, and the flip side of market oriented reforms. At the helm of one of Canada's largest schools is Arnold Aberman, MD, dean of the University of Toronto Faculty of Medicine. Born in Montreal, Quebec, Aberman received his MD from McGill University, but then did his residency both in Canada and the US, followed by a pulmonary fellowship at Albert Einstein College of Medicine and the Cardiovascular Research Institute of the University of California, San Francisco. Interviewed at his office in Toronto, Aberman reflected on the trials and tribulations confronting medicine on both sides of the 48th parallel.

  8. John M. Eisenberg, MD.

    PubMed

    Eisenberg, J M

    1995-08-01

    The complicated interaction between government, academic medical centers, health care payers, and burgeoning market forces has tested the leadership skills of a generation of academicians with little formal training in economics. The emergence of a new breed of physician investigator with solid business credentials has therefore proved attractive to many segments of the medical community. John M. Eisenberg received his MD from Washington University in 1972, his MBA from the Wharton School in 1976, and shortly thereafter headed the division of general internal medicine at the University of Pennsylvania. In addition to championing the role of the generalist in health care delivery, Eisenberg has also played a major part in the reformation of Medicare reimbursement. He has been a commissioner on the Congressional Physician Payment Review Commission since 1986, serving as chairman since 1993. After assuming the chairmanship of the department of medicine at Georgetown University in 1992, Eisenberg served as an advisor to the Clinton administration during its efforts towards national health care reform. Interviewed in his office in Georgetown, Eisenberg reflected on the economic forces twisting post-graduate medical education, the role of non-physician providers in future health care delivery, and the evolving relationship between specialists and generalists. PMID:7552581

  9. 78 FR 14547 - Praxedes E. Alverez Santiago, M.D., Daniel Perez Brisebois, M.D., Jorge Grillasca Palou, M.D...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-06

    ... Praxedes E. Alverez Santiago, M.D., Daniel Perez Brisebois, M.D., Jorge Grillasca Palou, M.D., Rafael..., M.D., Daniel P rez Brisebois, M.D., Jorge Grillasca Palou, M.D., Rafael Garc a Nieves, M.D., Francis... and Dr. Daniel Perez, met with Humana representatives to discuss the 20 percent COB. During...

  10. Measurement of the Md3+/Md2+ reduction potential studied with flow electrolytic chromatography.

    PubMed

    Toyoshima, Atsushi; Li, Zijie; Asai, Masato; Sato, Nozomi; Sato, Tetsuya K; Kikuchi, Takahiro; Kaneya, Yusuke; Kitatsuji, Yoshihiro; Tsukada, Kazuaki; Nagame, Yuichiro; Schädel, Matthias; Ooe, Kazuhiro; Kasamatsu, Yoshitaka; Shinohara, Atsushi; Haba, Hiromitsu; Even, Julia

    2013-11-01

    The reduction behavior of mendelevium (Md) was studied using a flow electrolytic chromatography apparatus. By application of the appropriate potentials on the chromatography column, the more stable Md(3+) is reduced to Md(2+). The reduction potential of the Md(3+) + e(-) → Md(2+) couple was determined to be -0.16 ± 0.05 V versus a normal hydrogen electrode. PMID:24116851

  11. Obituary of Philip H. Cooper, MD.

    PubMed

    Patterson, James W; Wick, Mark R; Mills, Stacey E

    2015-08-01

    Dermatopathology lost a giant in the field with the death of Philip H. Cooper, MD, on Friday, January 30, 2015. The following obituary represents a celebration of his life and his contributions to our field.

  12. VIEW IN OPPOSITE DIRECTION AS MD1351 AND MD1352. RAW MATERIAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW IN OPPOSITE DIRECTION AS MD-135-1 AND MD-135-2. RAW MATERIAL CONVEYOR AT LEFT DEPOSITS SHELL INTO MILLING MACHINE AT LOWER LEFT. ENGINE IS AT LOWER RIGHT AND RADIATOR AT LOWER CENTER. ROLLER SORTER IS AT TOP OF CONVEYOR. - F. & H. Benning Company Oyster Mill, 14430 Solomons Island Road (moved from 1014 Benning Road, Galesville, Anne Arundel County, Maryland), Solomons, Calvert County, MD

  13. New Message Differences for Collision Attacks on MD4 and MD5

    NASA Astrophysics Data System (ADS)

    Sasaki, Yu; Wang, Lei; Kunihiro, Noboru; Ohta, Kazuo

    In 2005, collision resistance of several hash functions was broken by Wang et al. The strategy of determining message differences is the most important part of collision attacks against hash functions. So far, many researchers have tried to analyze Wang et al.'s method and proposed improved collision attacks. Although several researches proposed improved attacks, all improved results so far were based on the same message differences proposed by Wang et al. In this paper, we propose new message differences for collision attacks on MD4 and MD5. Our message differences of MD4 can generate a collision with complexity of less than two MD4 computations, which is faster than the original Wang et al.'s attack, and moreover, than the all previous attacks. This is the first result that improves the complexity of collision attack by using different message differences from Wang et al.'s. Regarding MD5, so far, no other message difference from Wang et al.'s is known. Therefore, study for constructing method of other message differences on MD5 should be interesting. Our message differences of MD5 generates a collision with complexity of 242 MD5 computations, which is slower than the latest best attack. However, since our attack needs only 1 bit difference, it has some advantages in terms of message freedom of collision messages.

  14. 78 FR 2707 - Maryland Disaster # MD-00026

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-14

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Maryland Disaster MD-00026 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... Deadline Date: 10/02/2013. ADDRESSES: Submit completed loan applications to: U.S. Small...

  15. Howard Parnes, MD | Division of Cancer Prevention

    Cancer.gov

    Dr. Howard Parnes received a BA from Cornell University in 1977 and an MD from the University of Medicine and Dentistry of New Jersey in 1981. He trained in internal medicine at the Johns Hopkins Bayview Medical Center from 1981 to 1984 followed by a medical oncology fellowship at the University of Maryland Cancer Center (UMCC) from 1984 to 1987. |

  16. 76 FR 64421 - Maryland Disaster #MD-00018

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... ADMINISTRATION Maryland Disaster MD-00018 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for the... Disaster Assistance, U.S. Small Business Administration, 409 3rd Street, SW., Suite 6050, Washington,...

  17. 75 FR 79063 - Maryland Disaster #MD-00014

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-17

    ... ADMINISTRATION Maryland Disaster MD-00014 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Maryland dated 12/09... CONTACT: A. Escobar, Office of Disaster Assistance, U.S. Small Business Administration, 409 3rd Street,...

  18. 75 FR 27010 - Maryland Disaster #MD-00012

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... ADMINISTRATION Maryland Disaster MD-00012 AGENCY: Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for the... Disaster Assistance, U.S. Small Business Administration, 409 3rd Street, SW., Suite 6050, Washington,...

  19. 76 FR 77579 - Maryland Disaster #MD-00017

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-13

    ... ADMINISTRATION Maryland Disaster MD-00017 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Maryland, dated 12/07... INFORMATION CONTACT: A. Escobar, Office of Disaster Assistance, U.S. Small Business Administration, 409...

  20. The Multidimensional Curriculum Model (MdCM)

    ERIC Educational Resources Information Center

    Vidergor, Hava E.

    2010-01-01

    The multidimensional Curriculum Model (MdCM) helps teachers to better prepare gifted and able students for our changing world, acquiring much needed skills. It is influenced by general learning theory of constructivism, notions of preparing students for 21st century, Teaching the Future Model, and current comprehensive curriculum models for…

  1. 77 FR 76586 - Maryland Disaster #MD-00024

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-28

    ... ADMINISTRATION Maryland Disaster MD-00024 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for the State of Maryland (FEMA... Economic Injury Loans): Somerset. ] Contiguous Counties (Economic Injury Loans Only): Maryland:...

  2. 75 FR 9005 - Maryland Disaster #MD-00011

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Maryland Disaster MD-00011 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... the State of Maryland (FEMA- 1875-DR), dated 02/19/2010. Incident: Severe Winter Storm and...

  3. 76 FR 59478 - Maryland Disaster #MD-00016

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-26

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Maryland Disaster MD-00016 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of Maryland (FEMA- 4034-DR), dated 09/16/2011. Incident: Hurricane Irene. Incident Period:...

  4. 77 FR 48197 - Maryland Disaster #MD-00021

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Maryland Disaster MD-00021 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of Maryland (FEMA- 4075-DR), dated 08/02/2012. Incident: Severe storms and straight-line...

  5. MD-11 PCA - Research flight team photo

    NASA Technical Reports Server (NTRS)

    1995-01-01

    On Aug. 30, 1995, a the McDonnell Douglas MD-11 transport aircraft landed equipped with a computer-assisted engine control system that has the potential to increase flight safety. In landings at NASA Dryden Flight Research Center, Edwards, California, on August 29 and 30, the aircraft demonstrated software used in the aircraft's flight control computer that essentially landed the MD-11 without a need for the pilot to manipulate the flight controls significantly. In partnership with McDonnell Douglas Aerospace (MDA), with Pratt & Whitney and Honeywell helping to design the software, NASA developed this propulsion-controlled aircraft (PCA) system following a series of incidents in which hydraulic failures resulted in the loss of flight controls. This new system enables a pilot to operate and land the aircraft safely when its normal, hydraulically-activated control surfaces are disabled. This August 29, 1995, photo shows the MD-11 team. Back row, left to right: Tim Dingen, MDA pilot; John Miller, MD-11 Chief pilot (MDA); Wayne Anselmo, MD-11 Flight Test Engineer (MDA); Gordon Fullerton, PCA Project pilot; Bill Burcham, PCA Chief Engineer; Rudey Duran, PCA Controls Engineer (MDA); John Feather, PCA Controls Engineer (MDA); Daryl Townsend, Crew Chief; Henry Hernandez, aircraft mechanic; Bob Baron, PCA Project Manager; Don Hermann, aircraft mechanic; Jerry Cousins, aircraft mechanic; Eric Petersen, PCA Manager (Honeywell); Trindel Maine, PCA Data Engineer; Jeff Kahler, PCA Software Engineer (Honeywell); Steve Goldthorpe, PCA Controls Engineer (MDA). Front row, left to right: Teresa Hass, Senior Project Management Analyst; Hollie Allingham (Aguilera), Senior Project Management Analyst; Taher Zeglum, PCA Data Engineer (MDA); Drew Pappas, PCA Project Manager (MDA); John Burken, PCA Control Engineer.

  6. Direct NOE simulation from long MD trajectories.

    PubMed

    Chalmers, G; Glushka, J N; Foley, B L; Woods, R J; Prestegard, J H

    2016-04-01

    A software package, MD2NOE, is presented which calculates Nuclear Overhauser Effect (NOE) build-up curves directly from molecular dynamics (MD) trajectories. It differs from traditional approaches in that it calculates correlation functions directly from the trajectory instead of extracting inverse sixth power distance terms as an intermediate step in calculating NOEs. This is particularly important for molecules that sample conformational states on a timescale similar to molecular reorientation. The package is tested on sucrose and results are shown to differ in small but significant ways from those calculated using an inverse sixth power assumption. Results are also compared to experiment and found to be in reasonable agreement despite an expected underestimation of water viscosity by the water model selected. PMID:26826977

  7. Direct NOE simulation from long MD trajectories

    NASA Astrophysics Data System (ADS)

    Chalmers, G.; Glushka, J. N.; Foley, B. L.; Woods, R. J.; Prestegard, J. H.

    2016-04-01

    A software package, MD2NOE, is presented which calculates Nuclear Overhauser Effect (NOE) build-up curves directly from molecular dynamics (MD) trajectories. It differs from traditional approaches in that it calculates correlation functions directly from the trajectory instead of extracting inverse sixth power distance terms as an intermediate step in calculating NOEs. This is particularly important for molecules that sample conformational states on a timescale similar to molecular reorientation. The package is tested on sucrose and results are shown to differ in small but significant ways from those calculated using an inverse sixth power assumption. Results are also compared to experiment and found to be in reasonable agreement despite an expected underestimation of water viscosity by the water model selected.

  8. Gunpowder River Bridge. Harewood, Baltimore Co., MD. Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Gunpowder River Bridge. Harewood, Baltimore Co., MD. Sec. 1201, MP 78.86. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  9. Edgewood Interlocking Tower. Edgewood, Hareford Co., MD. Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Edgewood Interlocking Tower. Edgewood, Hareford Co., MD. Sec. 1201, MP 75.50. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  10. Pennsylvania Railroad: Pennsylvania Station. Baltimore, Baltimore City, MD. Sec. 1201, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Pennsylvania Railroad: Pennsylvania Station. Baltimore, Baltimore City, MD. Sec. 1201, MP 95.50. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  11. B & O Railroad: Bayview Bridge. Baltimore, Baltimore City, MD. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    B & O Railroad: Bayview Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP 92.02. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  12. Susquehanna River Bridge. Havre de Grace, Hareford Co., MD. Sec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Susquehanna River Bridge. Havre de Grace, Hareford Co., MD. Sec. 1201, MP 60.07. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  13. Edison Highway Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Edison Highway Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP 92.02. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  14. Gunpowder River Bridge drawspan. Harewood, Baltimore Co., MD. Sec. 1201, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Gunpowder River Bridge drawspan. Harewood, Baltimore Co., MD. Sec. 1201, MP 78.86. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  15. Wilkins Avenue Bridge. Violetville, Baltimore City, MD. Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Wilkins Avenue Bridge. Violetville, Baltimore City, MD. Sec. 1201, MP 100.59. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  16. Bush River Bridge drawspan. Bush River, Hareford Co., MD. Sec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Bush River Bridge drawspan. Bush River, Hareford Co., MD. Sec. 1201, MP 72.14. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  17. Bush River Bridge. Bush River, Hareford Co., MD. Sec. 1201, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Bush River Bridge. Bush River, Hareford Co., MD. Sec. 1201, MP 72.14. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  18. Juniata Street Culvert. Havre de Grace, Hareford Co., MD. Sec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Juniata Street Culvert. Havre de Grace, Hareford Co., MD. Sec. 1201, MP 60.77. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  19. LANDOVER SUBSTATION. LANDOVER, PRINCE GEORGES CO., MD Sec, 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    LANDOVER SUBSTATION. LANDOVER, PRINCE GEORGES CO., MD Sec, 1201, MP 129.00. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  20. BIG PATUXENT RIVER BRIDGE. ARUNDEL, ANNE ARUNDEL CO., MD. Sec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BIG PATUXENT RIVER BRIDGE. ARUNDEL, ANNE ARUNDEL CO., MD. Sec. 1201, MP 115.61. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  1. SEVERN RUN CULVERT. MAYFIELD, ANNE ARUNDEL CO., MD Sec. 1201, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SEVERN RUN CULVERT. MAYFIELD, ANNE ARUNDEL CO., MD Sec. 1201, MP 112.17. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  2. Gwynns Falls Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Gwynns Falls Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP 99.20. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  3. B & P Tunnel west portal. Baltimore, Baltimore City, MD. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    B & P Tunnel west portal. Baltimore, Baltimore City, MD. Sec. 1201, MP 97.43. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  4. CHESTNUT AVENUE BRIDGE. BOWIE, PRINCE GEORGES CO., MD. Sec. 1201,, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CHESTNUT AVENUE BRIDGE. BOWIE, PRINCE GEORGES CO., MD. Sec. 1201,, MP 120.48. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  5. Boothby Hill Road Bridge. Aberdeen, Hareford Co., MD. Sec. 1201, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Boothby Hill Road Bridge. Aberdeen, Hareford Co., MD. Sec. 1201, MP 66.88. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  6. Loudon Park Substation. Violetville, Baltimore City, MD. Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Loudon Park Substation. Violetville, Baltimore City, MD. Sec. 1201, MP 100.50. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  7. Union Junction Interlocking Tower. Baltimore, Baltimore City, MD. Sec. 1201, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Union Junction Interlocking Tower. Baltimore, Baltimore City, MD. Sec. 1201, MP 95.49. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  8. North Avenue Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North Avenue Bridge. Baltimore, Baltimore City, MD. Sec. 1201, MP 96.00. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  9. BOWIE SUBSTATION. BOWIE, PRINCE GEORGES CO., MD Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BOWIE SUBSTATION. BOWIE, PRINCE GEORGES CO., MD Sec. 1201, MP 129.55. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  10. Graphs for Isotopes of 101-Md (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides a graphic representation of nucleon separation energies and residual interaction parameters for isotopes of the chemical element 101-Md (Mendelevium, atomic number Z = 101).

  11. Toxic wastes discovered in Cecil County, MD

    SciTech Connect

    Not Available

    1994-01-01

    This report focuses on the clean-up activities following toxic waste contamination in Cecil County, MD. The potentially hazardous leakage from waste containers buried in a quarry was addressed before it was turned into a civil emergency of the magnitude of Love Canal. US Superfund is a federal government trust fund largely comprised of taxes on the chemical and petrochemical industries. Local governments share in the cost of removing the waste and other aspects of emergency management.

  12. Toxic wastes discovered in Cecil County, MD

    SciTech Connect

    1994-12-31

    This report focuses on the clean-up activities following toxic waste contamination in Cecil County, MD. The potentially hazardous leakage from waste containers buried in a quarry was addressed before it was turned into a civil emergency of the magnitude of Love Canal. US Superfund is a federal government trust fund largely comprised of taxes on the chemical and petrochemical industries. Local governments share in the cost of removing the waste and other aspects of emergency management.

  13. Eva Szabo, MD | Division of Cancer Prevention

    Cancer.gov

    Dr. Eva Szabo is Chief of the Lung and Upper Aerodigestive Cancer Research Group at the NCI Division of Cancer Prevention. She graduated from Yale University with a BS in Molecular Biophysics and Biochemistry, received her MD from Duke University, and completed her internal medicine residency at Bellevue-NYU Medical Center. After completing her medical oncology fellowship at the National Cancer Institute, Dr. Szabo led a laboratory effort studying lung cancer biology. |

  14. Remains of abutments for Bridge No. 1575 at MD Rt. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Remains of abutments for Bridge No. 1575 at MD Rt. 51 in Spring Gap, Maryland, looking northeast. (Compare with HAER MD-115 photos taken 1988). - Western Maryland Railway, Cumberland Extension, Pearre to North Branch, from WM milepost 125 to 160, Pearre, Washington County, MD

  15. Discovery of /sup 260/Md and the decay properties of /sup 258/Fm, /sup 258m,g/Md, and /sup 259/Md

    SciTech Connect

    Lougheed, R.W.; Hulet, E.K.; Dougan, R.J.; Wild, J.F.; Dupzyk, R.J.; Henderson, C.M.; Moody, K.J.; Hahn, R.L.; Suemmerer, K.; Bethune, G.

    1985-08-01

    We have discovered a new neutron-rich isotope, /sup 260/Md, from /sup 18/O and /sup 22/Ne bombardments of /sup 254/Es. We observed a spontaneous-fission (SF) activity with a 32-day half-life in electromagnetically separated mass-260 fractions from these bombardments and we have measured the mass and kinetic energy distributions of this SF activity. The mass distribution was symmetric with the principal energy peak at 234-MeV total kinetic energy (TKE), similar to previous observations for heavy Fm isotopes. Surprisingly, we also observed a smaller symmetric component with 195-MeV TKE. We interpret these two peaks in the TKE distribution as arising from two types of fission in the same nucleus, or bimodal fission. The observed fission activity may be either from the SF decay of /sup 260/Md or /sup 260/Fm which would arise from electron capture (EC) decay of /sup 260/Md. We have eliminated the possible ..beta../sup -/ decay of /sup 260/Md by measuring ..beta../sup -/-SF time correlations for the decay of /sup 260/Md and we plan to determine if /sup 260/Md decays by EC by measuring time correlations between Fm x-rays and SF events. We also measured properties for heavy Fm and Md isotopes which include: (1) more accurate cross sections for the neutron-rich Md isotopes which we use to predict the production rates of yet undiscovered nuclides; (2) improved half-live measurements for /sup 258m,g/Md and /sup 259/Md; (3) confirmation of the EC decay of /sup 258m/Md by measuring Fm x-rays preceding the SF decay of /sup 258/Fm; and (4) very substantially improved mass and TKE distributions for the SF decay of /sup 258/Fm and /sup 259/Md.

  16. ProtoMD: A prototyping toolkit for multiscale molecular dynamics

    NASA Astrophysics Data System (ADS)

    Somogyi, Endre; Mansour, Andrew Abi; Ortoleva, Peter J.

    2016-05-01

    ProtoMD is a toolkit that facilitates the development of algorithms for multiscale molecular dynamics (MD) simulations. It is designed for multiscale methods which capture the dynamic transfer of information across multiple spatial scales, such as the atomic to the mesoscopic scale, via coevolving microscopic and coarse-grained (CG) variables. ProtoMD can be also be used to calibrate parameters needed in traditional CG-MD methods. The toolkit integrates 'GROMACS wrapper' to initiate MD simulations, and 'MDAnalysis' to analyze and manipulate trajectory files. It facilitates experimentation with a spectrum of coarse-grained variables, prototyping rare events (such as chemical reactions), or simulating nanocharacterization experiments such as terahertz spectroscopy, AFM, nanopore, and time-of-flight mass spectroscopy. ProtoMD is written in python and is freely available under the GNU General Public License from github.com/CTCNano/proto_md.

  17. Gordon Fullerton in PCA (MD-11) Simulator

    NASA Technical Reports Server (NTRS)

    1998-01-01

    NASA research pilot Gordon Fullerton 'flying' in the MD-11 simulator during the Propulsion Controlled Aircraft (PCA) project. This investigation grew out of the crash of a DC-10 airliner on July 19, 1989, following an explosion in the rear engine which caused the loss of all manual flight controls. The flight crew attempted to control the airliner using only the thrust from the two remaining engines. Although the DC-10 crashed during the landing attempt, 184 of the 296 passengers and crew aboard survived. The PCA effort at the Dryden Flight Research Center grew out of the crash, and attempted to develop a means to successfully land an aircraft using only engine thrust. After more than five years of work, on August 29, 1995, Gordon Fullerton made the first PCA touchdown aboard an MD-11 airliner (a later version of the DC-10). The concept was further refined over the years that followed this first landing. Simulators were essential ingredients of the PCA development process. The feasibility of the concept was first tested with an F-15 simulator, then the results of actual flight tests in an F-15 were incorporated back into the simulator. Additional simulations were run on the Boeing 720 airliner simulator used in the Controlled Impact Demonstration project. After the MD-11 test landings, Boeing 747 and 757 simulators tested a wide range of possible situations. Simulations even helped develop a method of landing an airliner if it lost its complete hydraulic system as well as a wing engine, by transferring fuel to shift the center of gravity toward the working engine. The most extreme procedure was undertaken in a 747 simulator. The aircraft simulated the loss of the hydraulic system at 35,000 feet and rolled upside down. Then, the PCA mode was engaged, the airliner righted itself, leveled its wings, and made an approach nearly identical to that of a normal auto landing.

  18. Dawbeney Turbervile, MD (1612-1696).

    PubMed

    Simunovic, Matthew P

    2012-03-01

    The year 2012 marks the quatercentenary of the birth of Dawbeney Turbervile,MD(1612-1696), one-time Royalist soldier and later ophthalmologist to England’s Princess Anne, the diarist Samuel Pepys, the natural philosopher Robert Boyle, and the astronomer Walter Pope. Turbervile is remarkable for many reasons: He specialized at a time when generalization was prized; though he was a qualified physician, he also practiced the trade of surgery. Furthermore, he provided in his communications with the Royal Society early descriptions of achromatopsia, ocular foreign body removal with a magnet, and tic doloreaux. He is a forebear worth remembering

  19. Dawbeney Turbervile, MD (1612-1696).

    PubMed

    Simunovic, Matthew P

    2012-03-01

    The year 2012 marks the quatercentenary of the birth of Dawbeney Turbervile,MD(1612-1696), one-time Royalist soldier and later ophthalmologist to England’s Princess Anne, the diarist Samuel Pepys, the natural philosopher Robert Boyle, and the astronomer Walter Pope. Turbervile is remarkable for many reasons: He specialized at a time when generalization was prized; though he was a qualified physician, he also practiced the trade of surgery. Furthermore, he provided in his communications with the Royal Society early descriptions of achromatopsia, ocular foreign body removal with a magnet, and tic doloreaux. He is a forebear worth remembering PMID:22411681

  20. 75 FR 12441 - Airworthiness Directives; MD Helicopters, Inc. Model MD-900 Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... FR 42824, August 6, 1999), Docket No. 98-SW-42-AD, for the MDHI Model MD-900 helicopters was published in the Federal Register on October 22, 2009 (74 FR 54495). The action proposed to decrease the... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a...

  1. 75 FR 69862 - Airworthiness Directives; MD Helicopters, Inc. Model MD900 Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... beam bolt hole locations during maintenance on two MDHI Model MD900 helicopters. The actions specified... loss of control of the helicopter. DATES: Effective December 1, 2010, to all persons except those... AD was prompted by two reports of cracks detected in the hub in the area near the flex beam bolt...

  2. Benjamin Rush, MD: assassin or beloved healer?

    PubMed Central

    2000-01-01

    Benjamin Rush, MD (1745–1813), was not only the most well known physician in 18th-century America, he was also a patriot, philosopher, author, lecturer, fervent evangelist, politician, and dedicated social reformer. He was unshakable in his convictions, as well as self-righteous, caustic, satirical, humorless, and polemical. Unquestionably brilliant, he graduated from what later became Princeton University at age 14. He translated Hippocrates' Aphorisms from the Greek at age 17. He wrote the first textbook of chemistry to be published in America. He was by all accounts a devoted, if highly paternalistic, medical practitioner, who cared deeply for his patients' welfare. His principles or theories and his championship of extreme purging and bleeding (“depletion therapy”) have engendered 200 years of controversy and debate that continue today. The contradiction in his character is particularly well illustrated by his behavior during the Philadelphia yellow fever epidemic of 1793, as is briefly examined in this essay. PMID:16389324

  3. Benjamin Rush, MD: assassin or beloved healer?

    PubMed

    North, R L

    2000-01-01

    Benjamin Rush, MD (1745-1813), was not only the most well known physician in 18th-century America, he was also a patriot, philosopher, author, lecturer, fervent evangelist, politician, and dedicated social reformer. He was unshakable in his convictions, as well as self-righteous, caustic, satirical, humorless, and polemical. Unquestionably brilliant, he graduated from what later became Princeton University at age 14. He translated Hippocrates' Aphorisms from the Greek at age 17. He wrote the first textbook of chemistry to be published in America. He was by all accounts a devoted, if highly paternalistic, medical practitioner, who cared deeply for his patients' welfare. His principles or theories and his championship of extreme purging and bleeding ("depletion therapy") have engendered 200 years of controversy and debate that continue today. The contradiction in his character is particularly well illustrated by his behavior during the Philadelphia yellow fever epidemic of 1793, as is briefly examined in this essay. PMID:16389324

  4. Ashley W. Oughterson, MD: Surgeon, Soldier, Leader

    PubMed Central

    Kunstman, John W.; Longo, Walter E.

    2015-01-01

    Ashley W. Oughterson, MD, (1895-1956) was a longtime faculty surgeon at Yale University. He performed some of the earliest pancreatic resections in the United States. During World War II, Colonel Oughterson was the primary “Surgical Consultant” in the South Pacific and present at nearly every major battle. His meticulously kept diary is regarded as the foremost source detailing wartime surgical care. Colonel Oughterson led the initial Army team to survey Hiroshima and Nagasaki following the nuclear attacks. Thoughout his academic career at Yale, Oughterson was a key leader in several medical and surgical societies. As scientific director of the American Cancer Society, Oughterson lectured widely and guided research priorities in oncology following World War II. Oughterson also authored numerous benchmark papers in surgical oncology that continue to be cited today. These extensive contributions are examined here and demonstrate the wide-ranging impact Oughterson exerted during a formative period of American surgery. PMID:26029018

  5. 77 FR 24838 - Safety Zone; Magothy River, Sillery Bay, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-26

    ... ``Safety Zone; Magothy River, Sillery Bay, MD'' in the Federal Register (77 FR 11423). We received no... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Magothy River, Sillery Bay, MD AGENCY... waters of the Magothy River, in Sillery Bay, Maryland. This safety zone is necessary to provide for...

  6. 76 FR 51887 - Safety Zone; Patuxent River, Patuxent River, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ...) entitled ``Safety Zone; Patuxent River, Patuxent River, MD'' in the Federal Register (76 FR 36447). We... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Patuxent River, Patuxent River, MD AGENCY... safety zone during the ``NAS Patuxent River Air Expo '11,'' which consists of aerial...

  7. 77 FR 5201 - Drawbridge Operation Regulation; Bear Creek, Dundalk, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-02

    ... Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a public meeting. But you may... SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulation; Bear Creek, Dundalk, MD... across Bear Creek, mile 3.4, between Dundalk and Sparrows Point, MD. The proposed change will alter...

  8. 78 FR 35773 - Airworthiness Directives; MD Helicopters, Inc. (MDHI), Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-14

    ...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); 3. Will not affect... Directives; MD Helicopters, Inc. (MDHI), Helicopters AGENCY: Federal Aviation Administration, DOT. ACTION... the MDHI Model MD900 helicopters with certain main rotor blade (MRB) retention bolts (bolts)...

  9. 77 FR 42459 - Airworthiness Directives; MD Helicopters, Inc. (MDHI) Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-19

    ..., and MD900 helicopters to require determining the cure date for each NOTAR fan blade tension-torsion... (expiration date). For the MDHI Model MD900 helicopters, AD 2006-18-01 (71 FR 51095, August 29, 2006) already... these same type designs. Related Service Information We have reviewed one MDHI service bulletin,...

  10. 75 FR 10172 - Drawbridge Operation Regulation; Chester River, Chestertown, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-05

    ... drawbridge operation regulations of the S213 Bridge, at mile 26.8, across Chester River at Chestertown, MD. This final rule allows the bridge to open on signal if at least six hours notice is given and will... Operation Regulations; Chester River, Chestertown, MD'' in the Federal Register (74 FR 48889). We...

  11. 33 CFR 110.72 - Blackhole Creek, Md.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Blackhole Creek, Md. 110.72... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.72 Blackhole Creek, Md. The waters on the west side of Blackhole Creek, a tributary of Magothy River, southwest of a line bearing 310°30′ from the most...

  12. 33 CFR 110.72 - Blackhole Creek, Md.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Blackhole Creek, Md. 110.72... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.72 Blackhole Creek, Md. The waters on the west side of Blackhole Creek, a tributary of Magothy River, southwest of a line bearing 310°30′ from the most...

  13. 33 CFR 110.72 - Blackhole Creek, Md.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Blackhole Creek, Md. 110.72... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.72 Blackhole Creek, Md. The waters on the west side of Blackhole Creek, a tributary of Magothy River, southwest of a line bearing 310°30′ from the most...

  14. 33 CFR 110.72 - Blackhole Creek, Md.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Blackhole Creek, Md. 110.72... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.72 Blackhole Creek, Md. The waters on the west side of Blackhole Creek, a tributary of Magothy River, southwest of a line bearing 310°30′ from the most...

  15. 33 CFR 110.72 - Blackhole Creek, Md.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Blackhole Creek, Md. 110.72... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.72 Blackhole Creek, Md. The waters on the west side of Blackhole Creek, a tributary of Magothy River, southwest of a line bearing 310°30′ from the most...

  16. Leroy D Vandam, MD: an anesthesia journey.

    PubMed

    Ortega, Rafael A

    2005-08-01

    Leroy D Vandam, MD was a remarkable man--an intricate amalgamation of an artist, scientist, and physician. He was a bastion of medical historical knowledge. Dr Vandam became a most influential anesthesiologist, some say, a giant. He was an example of someone who, with resolve, overcame adversity. His artwork is displayed in countless places, and several of his paintings form part of the Wood Library Museum Heritage Series. Dr Vandam was first a surgeon, but he abandoned surgery and pursued a career in anesthesiology under the leadership of Robert Dripps. He completed his residency training at the University of Pennsylvania and joined its staff in 1949. When he arrived at Brigham and Women's Hospital in the 1950s as director of anesthesia, he embarked on one of the most illustrious careers in American anesthesiology. Dr Vandam published more than 250 original articles, chapters, abstracts, and other reports on a wide variety of subjects including history, art, and pharmacology. His classic article on the complications of neuroaxial blocks is a seminal work in anesthesiology. This article describes how an anesthesiologist who shared an interest with Dr Vandam in the history of anesthesiology came to produce a movie based on his career, the evolution of anesthesia equipment, and the transformation of our specialty. PMID:16102694

  17. Leroy D Vandam, MD: an anesthesia journey.

    PubMed

    Ortega, Rafael A

    2005-08-01

    Leroy D Vandam, MD was a remarkable man--an intricate amalgamation of an artist, scientist, and physician. He was a bastion of medical historical knowledge. Dr Vandam became a most influential anesthesiologist, some say, a giant. He was an example of someone who, with resolve, overcame adversity. His artwork is displayed in countless places, and several of his paintings form part of the Wood Library Museum Heritage Series. Dr Vandam was first a surgeon, but he abandoned surgery and pursued a career in anesthesiology under the leadership of Robert Dripps. He completed his residency training at the University of Pennsylvania and joined its staff in 1949. When he arrived at Brigham and Women's Hospital in the 1950s as director of anesthesia, he embarked on one of the most illustrious careers in American anesthesiology. Dr Vandam published more than 250 original articles, chapters, abstracts, and other reports on a wide variety of subjects including history, art, and pharmacology. His classic article on the complications of neuroaxial blocks is a seminal work in anesthesiology. This article describes how an anesthesiologist who shared an interest with Dr Vandam in the history of anesthesiology came to produce a movie based on his career, the evolution of anesthesia equipment, and the transformation of our specialty.

  18. DFT-MD simulations of shocked Xenon

    NASA Astrophysics Data System (ADS)

    Magyar, Rudolph J.; Mattsson, Thomas R.

    2009-03-01

    Xenon is not only a technologically important element used in laser technologies, jet propulsion and dental anesthesia, but it is also arguably the simplest material in which to study the metal-insulator transition at high pressure. Because of its closed shell electronic configuration, Xenon is often assumed to be chemically inert, interacting almost entirely through the van der Waals interaction, and at liquid density, is typically modeled well using Leonard-Jones potentials. However, such modeling has a limited range of validity as Xenon is known to form compounds at normal conditions and likely exhibits considerably more chemistry at higher densities when hybridization of occupied orbitals becomes significant. In this talk, we present DFT-MD simulations of shocked liquid Xenon with the goal of developing an improved equation of state. The relative importance of the van der Waals interaction compared to other Coulomb interactions is considered, and estimates of the relative accuracy of various density functionals are quantified. Sandia is a multiprogram laboratory operated by Sandia Corporation, a Lockheed Martin Company, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.

  19. 33 CFR 334.180 - Patuxent River, Md.; restricted areas, U.S. Naval Air Test Center, Patuxent River, Md.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Patuxent River, Md.; restricted areas, U.S. Naval Air Test Center, Patuxent River, Md. 334.180 Section 334.180 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED...

  20. Gene Expression Profiling in rMd5- and rMd5-delta-meq-Infected Chickens

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Marek’s disease (MD) is a lymphoproliferative disorder of domestic chickens caused by a highly contagious and oncogenic alpha-herpesvirus, Marek’s disease virus (MDV). MD is characterized by bursal/thymic atrophy and rapid onset of T cell lymphomas that infiltrate lymphoid tissues, visceral organs,...

  1. 75 FR 12464 - Airworthiness Directives; McDonnell Douglas Corporation Model MD-11 and MD-11F Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ...'' (66 FR 23086, May 7, 2001). In addition to new airworthiness standards for transport airplanes and new... Procedures (44 FR 11034, February 26, 1979), and 3. Will not have a significant economic impact, positive or... Directives; McDonnell Douglas Corporation Model MD- 11 and MD-11F Airplanes AGENCY: Federal...

  2. MD-PhD training: looking back and looking forward.

    PubMed

    Bonham, Ann C

    2014-01-01

    MD-PhD programs provide rigorous, integrated training for physician-scientists, enabling them to frame scientific questions in unique ways and to apply clinical insight to fundamental science. Few would question the influential contributions of MD-PhD physician-scientists in advancing medical science. In this issue of Academic Medicine, Jeffe et al affirm high levels of excellence in educational outcomes from MD-PhD training programs at U.S. MD-granting medical schools, especially programs that receive funding from the NIH Medical Scientist Training Program (MSTP). The author of this commentary observes that, in the face of current economic pressures, comprehensive, longitudinal national outcomes data from MSTP- and non-MSTP-funded MD-PhD programs will help verify the value provided by MD-PhD physician-scientists. She proposes that MD-PhD programs should better prepare the next generation of physician-scientists for future research environments, which will provide new technologies, venues, and modalities. These research environments will be more closely integrated within health care delivery systems, extend into diverse communities and regions, and employ complex technologies. MD-PhD physician-scientists also will train and gain expertise in broadening areas of research, such as health policy, health economics, clinical epidemiology, and medical informatics. Program leaders are ideally situated to foster innovative learning environments and methodologies. By sharing their innovations, they can help ensure production of a diverse MD-PhD physician-scientist workforce, prepared to engage in myriad research opportunities to meet patient and population needs in a new environment. PMID:24280863

  3. MD-PhD training: looking back and looking forward.

    PubMed

    Bonham, Ann C

    2014-01-01

    MD-PhD programs provide rigorous, integrated training for physician-scientists, enabling them to frame scientific questions in unique ways and to apply clinical insight to fundamental science. Few would question the influential contributions of MD-PhD physician-scientists in advancing medical science. In this issue of Academic Medicine, Jeffe et al affirm high levels of excellence in educational outcomes from MD-PhD training programs at U.S. MD-granting medical schools, especially programs that receive funding from the NIH Medical Scientist Training Program (MSTP). The author of this commentary observes that, in the face of current economic pressures, comprehensive, longitudinal national outcomes data from MSTP- and non-MSTP-funded MD-PhD programs will help verify the value provided by MD-PhD physician-scientists. She proposes that MD-PhD programs should better prepare the next generation of physician-scientists for future research environments, which will provide new technologies, venues, and modalities. These research environments will be more closely integrated within health care delivery systems, extend into diverse communities and regions, and employ complex technologies. MD-PhD physician-scientists also will train and gain expertise in broadening areas of research, such as health policy, health economics, clinical epidemiology, and medical informatics. Program leaders are ideally situated to foster innovative learning environments and methodologies. By sharing their innovations, they can help ensure production of a diverse MD-PhD physician-scientist workforce, prepared to engage in myriad research opportunities to meet patient and population needs in a new environment.

  4. Michael S. Brown, MD and Joseph L. Goldstein, MD. 1985 Nobel laureates in medicine.

    PubMed

    Brown, M S; Goldstein, J L

    1996-02-01

    When Michael S. Brown, MD and Joseph L. Goldstein, MD first met as interns at the Massachusetts General Hospital in 1966, they could hardly have imagined that their careers would continue to be intertwined some 30 years later. It was shortly following their arrival as clinical associates at the National Institutes of Health in 1968 that the pair developed an interest in abnormalities of cholesterol metabolism. Bolstered by epidemiologic data that showed elevated cholesterol levels in many patients with myocardial infarction, Brown and Goldstein, who relocated to the University of Texas Southwestern Medical Center in 1972, began a search for receptors important in cholesterol homeostasis. These studies, performed in their early stages while juggling clinical duties at Parkland Hospital, culminated in a series of scientific achievements which merited among other honors the Hazen Award in 1982, the Lasker Award in 1985, and the Nobel Prize in Medicine in 1985. Today, as Regental Professors of the University of Texas, Brown and Goldstein head a laboratory group which continues to test the cutting edge of medical research. Although impressed with the pace of technological advances in biology, the declining role of clinically oriented physicians in biomedical research troubles the pair. Interviewed in their library in Dallas, Brown and Goldstein spoke about the complicated balance of science, medicine, and education necessary to produce another generation of successful investigators.

  5. Find an Orthopaedic Foot and Ankle MD/DO

    MedlinePlus

    ... AOFAS / FootCareMD / Find a Surgeon Find an Orthopaedic Foot & Ankle Surgeon Page Content The Orthopaedic Distinction Who are Orthopaedic Foot & Ankle Surgeons? Orthopaedic foot and ankle surgeons are ...

  6. Two types of spermine synthase gene: MdACL5 and MdSPMS are differentially involved in apple fruit development and cell growth.

    PubMed

    Kitashiba, Hiroyasu; Hao, Yu-Jin; Honda, Chikako; Moriguchi, Takaya

    2005-11-21

    Three cDNAs with high homology to spermine (Spm) synthases in Arabidopsis were isolated from apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.]. MdACL5-1 and MdACL5-2 have high homology with ACL5 and MdSPMS has high homology with AtSPMS. The similarity of MdSPMS to spermidine synthases (SPDSs) was higher than that of MdACL5s, despite the fact that both are putative Spm synthases. However, MdSPMS could be discriminated from SPDSs by the presence of several characteristic amino acids, i.e., Val-149, Ser-161, Ala-205, and Val-235, in the decarboxylated S-adenosylmethionine (dcSAM)-binding motif of MdSPMS. Both MdACL5-1 and MdSPMS complemented Spm biosynthesis in a yeast mutant deficient in Spm synthase, and ectopic expression of MdACL5-1 in the Arabidopsis dwarf mutant acl5 allowed recovery of the normal phenotype. RNA gel blot analysis showed that MdACL5 and MdSPMS are differentially expressed in tissues and suspension cells. These results suggest that functional MdACL5 and MdSPMS are independently involved in apple fruit development and cell growth.

  7. 75 FR 47203 - Airworthiness Directives; McDonnell Douglas Corporation Model MD-11 and MD-11F Airplanes Equipped...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-05

    ... Corporation Model MD- 11 and MD-11F Airplanes Equipped With General Electric CF6-80C2 Series Engines AGENCY..., equipped with General Electric Model CF6-80C2 or CF6-80A series engines. These airplanes have been... partially agree with the commenter's request. We agree that the General Electric (GE) CF6-80C2 series...

  8. MdSOS2L1 forms a complex with MdMYB1 to control vacuolar pH by transcriptionally regulating MdVHA-B1 in apples.

    PubMed

    Sun, Cui-Hui; Zhang, Quan-Yan; Sun, Mei-Hong; Hu, Da-Gang

    2016-01-01

    Vacuolar pH is important and involves in many different physiological processes in plants. A recent paper published in Plant Physiology reveals that MdMYB1 regulates vacuolar pH by directly transcriptionally regulating proton pump genes and malate transporters genes, such as V-ATPase subunit gene MdVHA-B1. Here, we found that MdSOS2L1 in vitro did not directly interact with MdMYB1, however, in vivo formed a complex with MdMYB1 in the nucleus to regulate MdVHA-B1-mediated vacuolar acidification. This finding shed light on the role of MdSOS2L1 in transcriptionally regulating MdVHA-B1 in addition to its post-modified function in apples. PMID:26910596

  9. 76 FR 53346 - Airworthiness Directives; The Boeing Company Model DC-9-81 (MD-81), DC-9-82 (MD-82), DC-9-83 (MD...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-26

    ... proposed AD affects Model MD-80 series airplanes. We issued AD 2011-01-11, Amendment 39-16565 (76 FR 430... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... hinge bearing lugs of the aft face of the center section of the horizontal stabilizer; measuring...

  10. Cummins MD & HD Accessory Hybridization CRADA -Annual Report FY15

    SciTech Connect

    Deter, Dean D.

    2015-10-01

    There are many areas of MD and HD vehicles that can be improved by new technologies and optimized control strategies. Component optimization and idle reduction need to be addressed, this is best done by a two part approach that includes selecting the best component technology, and/or architecture, and optimized controls that are vehicle focused. While this is a common focus in the light duty industry it has been gaining momentum in the MD and HD market as the market gets more competitive and the regulations become more stringent. When looking into systems optimization and idle reduction technologies, affected vehicle systems must first be considered, and if possible included in the new architecture to get the most benefit out of these new capabilities. Typically, when looking into idle reduction or component optimization for MD/HD, the vehicle s accessories become a prime candidate for electrification or hybridization. While this has already been studied on light duty vehicles (especially on hybrids and electric vehicles) it has not made any head way or market penetration in most MD and HD applications. If hybrids and electric MD and HD vehicles begin to break into the market this would be a necessary step into the ability to make those vehicles successful by allowing for independent, optimized operation separate from the engine.

  11. 76 FR 9965 - Amendment of Class E Airspace and Revocation of Class E Airspace; Easton, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-23

    ... surface, and remove Class E airspace designated as an extension to Class D airspace at Easton, MD (75 FR... Class E airspace designated as an extension to Class D surface area, and, therefore, will be removed for... Designated as Surface Areas. * * * * * AEA MD E2 Easton, MD Easton Airport/Newnam Field, MD (Lat. 38...

  12. Apple MdACS6 Regulates Ethylene Biosynthesis During Fruit Development Involving Ethylene-Responsive Factor.

    PubMed

    Li, Tong; Tan, Dongmei; Liu, Zhi; Jiang, Zhongyu; Wei, Yun; Zhang, Lichao; Li, Xinyue; Yuan, Hui; Wang, Aide

    2015-10-01

    Ethylene biosynthesis in plants involves different 1-aminocyclopropane-1-carboxylic acid synthase (ACS) genes. The regulation of each ACS gene during fruit development is unclear. Here, we characterized another apple (Malus×domestica) ACS gene, MdACS6. The transcript of MdACS6 was observed not only in fruits but also in other tissues. During fruit development, MdACS6 was initiated at a much earlier stage, whereas MdACS3a and MdACS1 began to be expressed at 35 d before harvest and immediateley after harvest, respectively. Moreover, the enzyme activity of MdACS6 was significantly lower than that of MdACS3a and MdACS1, accounting for the low ethylene biosynthesis in young fruits. Overexpression of MdACS6 (MdACS6-OE) by transient assay in apple showed enhanced ethylene production, and MdACS3a was induced in MdACS6-OE fruits but not in control fruits. In MdACS6 apple fruits silenced by the virus-induced gene silencing (VIGS) system (MdACS6-AN), neither ethylene production nor MdACS3a transcript was detectable. In order to explore the mechanism through which MdACS3a was induced in MdACS6-OE fruits, we investigated the expression of apple ethylene-responsive factor (ERF) genes. The results showed that the expression of MdERF2 was induced in MdACS6-OE fruits and inhibited in MdACS6-AN fruits. Yeast one-hybrid assay showed that MdERF2 protein could bind to the promoter of MdACS3a. Moreover, down-regulation of MdERF2 in apple flesh callus led to a decrease of MdACS3a expression, demonstrating the regulation of MdERF2 on MdACS3a. The mechanism through which MdACS6 regulates the action of MdACS3a was discussed.

  13. CoMD Implementation Suite in Emerging Programming Models

    2014-09-23

    CoMD-Em is a software implementation suite of the CoMD [4] proxy app using different emerging programming models. It is intended to analyze the features and capabilities of novel programming models that could help ensure code and performance portability and scalability across heterogeneous platforms while improving programmer productivity. Another goal is to provide the authors and venders with some meaningful feedback regarding the capabilities and limitations of their models. The actual application is a classical molecularmore » dynamics (MD) simulation using either the Lennard-Jones method (LJ) or the embedded atom method (EAM) for primary particle interaction. The code can be extended to support alternate interaction models. The code is expected ro run on a wide class of heterogeneous hardware configurations like shard/distributed/hybrid memory, GPU's and any other platform supported by the underlying programming model.« less

  14. 3MdB: the Mexican Million Models database

    NASA Astrophysics Data System (ADS)

    Morisset, C.; Delgado-Inglada, G.

    2014-10-01

    The 3MdB is an original effort to construct a large multipurpose database of photoionization models. This is a more modern version of a previous attempt based on Cloudy3D and IDL tools. It is accessed by MySQL requests. The models are obtained using the well known and widely used Cloudy photoionization code (Ferland et al, 2013). The database is aimed to host grids of models with different references to identify each project and to facilitate the extraction of the desired data. We present here a description of the way the database is managed and some of the projects that use 3MdB. Anybody can ask for a grid to be run and stored in 3MdB, to increase the visibility of the grid and the potential side applications of it.

  15. CoMD Implementation Suite in Emerging Programming Models

    SciTech Connect

    Haque, Riyaz; Reeve, Sam; Juallmes, Luc; Asal, Sameer Abu; Landmehr, Aaron; Gaffer, Sanian; Teodor Bercea, Gheorghe; Rubinstein, Zach

    2014-09-23

    CoMD-Em is a software implementation suite of the CoMD [4] proxy app using different emerging programming models. It is intended to analyze the features and capabilities of novel programming models that could help ensure code and performance portability and scalability across heterogeneous platforms while improving programmer productivity. Another goal is to provide the authors and venders with some meaningful feedback regarding the capabilities and limitations of their models. The actual application is a classical molecular dynamics (MD) simulation using either the Lennard-Jones method (LJ) or the embedded atom method (EAM) for primary particle interaction. The code can be extended to support alternate interaction models. The code is expected ro run on a wide class of heterogeneous hardware configurations like shard/distributed/hybrid memory, GPU's and any other platform supported by the underlying programming model.

  16. Automatic distributed workflow generation with GridMD library

    NASA Astrophysics Data System (ADS)

    Morozov, I. V.; Valuev, I. A.

    2011-09-01

    GridMD is a C++ class library intended for constructing simulation applications and running them in distributed environments. The library abstracts away from details of distributed environments, so that almost no knowledge of distributed computing is required from a physicist working with the library. She or he just uses GridMD function calls inside the application C++ code to perform parameter sweeps or other tasks that can be distributed at run-time. In this paper we briefly review the GridMD architecture. We also describe the job manager component which submits jobs to a remote system. The C++ source code of our PBS job manager may be used as a standalone tool and it is freely available as well as the full library source code. As illustrative examples we use simple expression evaluation codes and the real application of Coulomb cluster explosion simulation by Molecular Dynamics.

  17. 76 FR 78695 - Barry M. Schultz, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    .... [See Michael G. Dolin, M.D., 65 FR 5,661 (DEA 2000); Jesus R. Juarez, M.D., 62 FR 14,945 (DEA 1997... 1 (citing 21 U.S.C. 801(21), 823(f), 824(a)(3); Layfe Robert Anthony, M.D., 67 FR 20,346 (2009... Roy Chi Lung, M.D., 74 FR 20,346 (2009); Michael Chait, M.D., 73 FR 40,382 (2008); Shahid...

  18. MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples.

    PubMed

    An, Xiu-Hong; Tian, Yi; Chen, Ke-Qin; Liu, Xiao-Juan; Liu, Dan-Dan; Xie, Xing-Bin; Cheng, Cun-Gang; Cong, Pei-Hua; Hao, Yu-Jin

    2015-04-01

    Anthocyanin and proanthocyanidin (PA) are important secondary metabolites and beneficial to human health. Their biosynthesis is induced by jasmonate (JA) treatment and regulated by MYB transcription factors (TFs). However, which and how MYB TFs regulate this process is largely unknown in apple. In this study, MdMYB9 and MdMYB11 which were induced by methyl jasmonate (MeJA) were functionally characterized. Overexpression of MdMYB9 or MdMYB11 promoted not only anthocyanin but also PA accumulation in apple calluses, and the accumulation was further enhanced by MeJA. Subsequently, yeast two-hybrid, pull-down and bimolecular fluorescence complementation assays showed that both MYB proteins interact with MdbHLH3. Moreover, Jasmonate ZIM-domain (MdJAZ) proteins interact with MdbHLH3. Furthermore, chromatin immunoprecipitation-quantitative PCR and yeast one-hybrid assays demonstrated that both MdMYB9 and MdMYB11 bind to the promoters of ANS, ANR and LAR, whereas MdbHLH3 is recruited to the promoters of MdMYB9 and MdMYB11 and regulates their transcription. In addition, transient expression assays indicated that overexpression of MdJAZ2 inhibits the recruitment of MdbHLH3 to the promoters of MdMYB9 and MdMYB11. Our findings provide new insight into the mechanism of how MeJA regulates anthocyanin and PA accumulation in apple. PMID:25527830

  19. MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples.

    PubMed

    An, Xiu-Hong; Tian, Yi; Chen, Ke-Qin; Liu, Xiao-Juan; Liu, Dan-Dan; Xie, Xing-Bin; Cheng, Cun-Gang; Cong, Pei-Hua; Hao, Yu-Jin

    2015-04-01

    Anthocyanin and proanthocyanidin (PA) are important secondary metabolites and beneficial to human health. Their biosynthesis is induced by jasmonate (JA) treatment and regulated by MYB transcription factors (TFs). However, which and how MYB TFs regulate this process is largely unknown in apple. In this study, MdMYB9 and MdMYB11 which were induced by methyl jasmonate (MeJA) were functionally characterized. Overexpression of MdMYB9 or MdMYB11 promoted not only anthocyanin but also PA accumulation in apple calluses, and the accumulation was further enhanced by MeJA. Subsequently, yeast two-hybrid, pull-down and bimolecular fluorescence complementation assays showed that both MYB proteins interact with MdbHLH3. Moreover, Jasmonate ZIM-domain (MdJAZ) proteins interact with MdbHLH3. Furthermore, chromatin immunoprecipitation-quantitative PCR and yeast one-hybrid assays demonstrated that both MdMYB9 and MdMYB11 bind to the promoters of ANS, ANR and LAR, whereas MdbHLH3 is recruited to the promoters of MdMYB9 and MdMYB11 and regulates their transcription. In addition, transient expression assays indicated that overexpression of MdJAZ2 inhibits the recruitment of MdbHLH3 to the promoters of MdMYB9 and MdMYB11. Our findings provide new insight into the mechanism of how MeJA regulates anthocyanin and PA accumulation in apple.

  20. David Kasner, MD, and the Road to Pars Plana Vitrectomy.

    PubMed

    Blodi, Christopher F

    2016-01-01

    David Kasner, MD (1927-2001), used his extensive dissections of eye bank eyes and experiences in teaching cataract surgery to resident physicians to realize that excision of vitreous when present in the anterior chamber of eyes undergoing cataract surgery was preferable to prior intraoperative procedures. Noting that eyes tolerated his maneuvers, he then performed planned subtotal open-sky vitrectomies; first on a traumatized eye in 1961, then on two eyes of patients with amyloidosis (1966-1967). The success of these operations was noted by others, most particularly Robert Machemer, MD. Kasner's work directly led to further surgical developments, including closed pars plana vitrectomy. PMID:27660504

  1. David Kasner, MD, and the Road to Pars Plana Vitrectomy

    PubMed Central

    Blodi, Christopher F.

    2016-01-01

    David Kasner, MD (1927–2001), used his extensive dissections of eye bank eyes and experiences in teaching cataract surgery to resident physicians to realize that excision of vitreous when present in the anterior chamber of eyes undergoing cataract surgery was preferable to prior intraoperative procedures. Noting that eyes tolerated his maneuvers, he then performed planned subtotal open-sky vitrectomies; first on a traumatized eye in 1961, then on two eyes of patients with amyloidosis (1966–1967). The success of these operations was noted by others, most particularly Robert Machemer, MD. Kasner’s work directly led to further surgical developments, including closed pars plana vitrectomy. PMID:27660504

  2. David Kasner, MD, and the Road to Pars Plana Vitrectomy

    PubMed Central

    Blodi, Christopher F.

    2016-01-01

    David Kasner, MD (1927–2001), used his extensive dissections of eye bank eyes and experiences in teaching cataract surgery to resident physicians to realize that excision of vitreous when present in the anterior chamber of eyes undergoing cataract surgery was preferable to prior intraoperative procedures. Noting that eyes tolerated his maneuvers, he then performed planned subtotal open-sky vitrectomies; first on a traumatized eye in 1961, then on two eyes of patients with amyloidosis (1966–1967). The success of these operations was noted by others, most particularly Robert Machemer, MD. Kasner’s work directly led to further surgical developments, including closed pars plana vitrectomy.

  3. Single fiber electromyography (SFEMG) in mitochondrial diseases (MD).

    PubMed

    Cruz-Martínez, A; Arpa, J; Santiago, S; Pérez-Conde, C; Gutiérrez-Molina, M; Campos, Y

    2004-01-01

    Conventional EMG, nerve conduction studies and SFEMG were performed in 18 patients with various phenotypes of MD. 14 cases showed findings consistent with mild myopathy, 2 patients signs of sensory-motor axonal neuropathy and 2 cases a mixture of myopathy and axonal neuropathy. Motor unit fiber density was mild increased in 8 out of 13 tested cases. Jitter was abnormal in 10 out of 18 tested patients. Jitter abnormalities were not related to myopathic or neurogenic features in the EMG study, and may be observed in muscles without clinical weakness. The results suggest the existence of neuromuscular transmission disturbances in patients with MD.

  4. Edward Jenner, MD, and the scourge that was.

    PubMed

    White, P J; Shackelford, P G

    1983-09-01

    This article was inspired by the global eradication of smallpox in 1980. Rather than reiterate the recent history of this remarkable achievement, we have chosen to celebrate the event by reviewing the early history of smallpox immunization and the contribution of Edward Jenner, MD, to this endeavor. In addition, we present these historical events within the context of knowledge acquired by modern virologists.

  5. 77 FR 64709 - Airworthiness Directives; MD Helicopters, Inc.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-23

    ..., at 77 FR 18963, the Federal Register published our notice of proposed rulemaking (NPRM), which... Order 12866; (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034..., Amendment 39-16740 (76 FR 41662, July 15, 2011), and adding the following new AD: 2012-21-01 MD...

  6. 75 FR 54069 - Drawbridge Operation Regulations; Curtis Creek, Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-03

    ... (75 FR 30747-30750). The rulemaking concerned eliminating the need for a bridge tender by allowing the... SECURITY Coast Guard 33 CFR Part 117 RIN 1625--AA09 Drawbridge Operation Regulations; Curtis Creek... Avenue Bridge, at mile 0.9, across Curtis Creek at Baltimore, MD. The requested change would have...

  7. 77 FR 12476 - Drawbridge Operation Regulation; Curtis Creek, Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulation; Curtis Creek, Baltimore..., across Curtis Creek, mile 1.0, at Baltimore, MD. This deviation allows the bridge to operate on...

  8. 75 FR 50707 - Drawbridge Operation Regulation; Curtis Creek, Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-17

    ... SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulation; Curtis Creek, Baltimore... operation of the Pennington Avenue Bridge, across Curtis Creek, mile 0.9, at Baltimore, MD. This deviation... vessels bound for the Coast Guard Yard at Curtis Bay, as well as a significant amount of commercial...

  9. 76 FR 9225 - Drawbridge Operation Regulation; Curtis Creek, Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ... Regulations; Curtis Creek, Baltimore, MD'' in the Federal Register (74 FR 50707). The temporary deviation... SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulation; Curtis Creek, Baltimore... changing the drawbridge operation regulations of the Pennington Avenue Bridge, across Curtis Creek, mile...

  10. 75 FR 1705 - Drawbridge Operation Regulations; Curtis Creek, Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-13

    ... SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulations; Curtis Creek... operation of the I695 Bridge across Curtis Creek, mile 0.9, at Baltimore, MD. The deviation is necessary to... section of Curtis Creek and the bridge will not be able to open in the event of an emergency. Coast...

  11. 77 FR 73967 - Drawbridge Operation Regulation; Bear Creek, Dundalk, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-12

    ... Regulation; Bear Creek, Dundalk, MD'' in the Federal Register (77 FR 5201). The rulemaking concerned would... proposed rulemaking the bridge owner displayed on the Wise Avenue Bridge signage that stated a 48-hour advance notice was required to open the draw bridge. This signage portrayed improper...

  12. 76 FR 70528 - Maryland Disaster Number MD-00018

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... ADMINISTRATION Maryland Disaster Number MD-00018 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Only for the State of Maryland (FEMA-4038-DR), dated 10/05/2011 . Incident: Remnants of Tropical Storm... Private Non-Profit organizations in the State of Maryland, dated 10/05/2011, is hereby amended to...

  13. 78 FR 11725 - Maryland Disaster Number MD-00024

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-19

    ... ADMINISTRATION Maryland Disaster Number MD-00024 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Maryland (FEMA-4091-DR), dated 12/14/ 2012. Incident: Hurricane Sandy. Incident Period: 10/26/2012 through... the President's major disaster declaration for the State of Maryland, dated 12/14/2012 is...

  14. 75 FR 20400 - Maryland Disaster Number MD-00011

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-19

    ... ADMINISTRATION Maryland Disaster Number MD-00011 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Only for the State of MARYLAND (FEMA-1875-DR), dated 02/19/2010. Incident: Severe Winter Storm and... Private Non-Profit organizations in the State of Maryland, dated 02/19/2010, is hereby amended to...

  15. 76 FR 70527 - Maryland Disaster Number MD-00018

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... ADMINISTRATION Maryland Disaster Number MD-00018 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Only for the State of Maryland (FEMA-4038-DR), dated 10/05/2011. Incident: Remnants of Tropical Storm... disaster declaration for Private Non-Profit organizations in the State of Maryland, dated 10/05/2011,...

  16. 77 FR 74908 - Maryland Disaster Number MD-00025

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-18

    ... ADMINISTRATION Maryland Disaster Number MD-00025 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Only for the State of MARYLAND (FEMA-4091-DR), dated 11/20/2012. Incident: Hurricane Sandy. Incident... Non-Profit organizations in the State of MARYLAND, dated 11/20/2012, is hereby amended to include...

  17. 78 FR 3496 - Maryland Disaster Number MD-00025

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-16

    ... ADMINISTRATION Maryland Disaster Number MD-00025 AGENCY: U.S. Small Business Administration. ACTION: Amendment 3... Only for the State of Maryland (FEMA-4091-DR), dated 11/20/2012. Incident: Hurricane Sandy. Incident... Non-Profit organizations in the State of Maryland, dated 11/20/2012, is hereby amended to include...

  18. 77 FR 76587 - Maryland Disaster Number MD-00025

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-28

    ... ADMINISTRATION Maryland Disaster Number MD-00025 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Only for the State of Maryland (FEMA-4091-DR), dated 11/20/2012. Incident: Hurricane Sandy. Incident... Non-Profit organizations in the State of Maryland, dated 11/20/2012, is hereby amended to include...

  19. 76 FR 67245 - Maryland Disaster Number MD-00016

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... ADMINISTRATION Maryland Disaster Number MD-00016 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Only for the State of Maryland (FEMA-4034-DR), dated 09/16/2011. Incident: Hurricane Irene. Incident... Non-Profit organizations in the State of Maryland, dated 09/16/2011, is hereby amended to include...

  20. Spectroscopic studies beyond N = 152 neutron gap : decay of {sup 255 ovr sub 101}Md and {sup 256 ovr sub 101}Md.

    SciTech Connect

    Ahmad, I.; Chasman, R. R.; Fields, P. R.

    2000-01-01

    The isotopes {sup 255}Md and {sup 256}Md were produced by the irradiation of {sup 253}Es with 35-45 MeV {alpha} particles by ({alpha},n) and ({alpha},2n) reactions and were removed from the target by a helium jet system. {alpha}, {gamma}, and {alpha}-{gamma} coincidence spectra were measured with Si and Ge(Li) detectors. From the EC decays of {sup 255}Md and {sup 256}Md, levels in {sup 255}Fm and {sup 256}Fm were deduced. Favored {alpha} decay of {sup 255}Md was found to populate the 7/2{sup -}[514] single-particle state in {sup 251}Es, thus establishing the 7/2{sup -}[514] as the {sup 255}Md ground state. Several {gamma} rays were observed in the {sub 256}Md {alpha}-{gamma} coincidence spectrum. {sup 256}Fm is the heaviest nucleus in which excited intrinsic states have been identified.

  1. Spectroscopic studies beyond the N=152 neutron gap: Decay of {sub 101}{sup 255}Md and {sub 101}{sup 256}Md

    SciTech Connect

    Ahmad, I.; Chasman, R. R.; Fields, P. R.

    2000-04-01

    The isotopes {sup 255}Md and {sup 256}Md were produced by the irradiation of {sup 253}Es with 35-45 MeV {alpha} particles by ({alpha},n) and ({alpha},2n) reactions and were removed from the target by a helium jet system. {alpha}, {gamma}, and {alpha}-{gamma} coincidence spectra were measured with Si and Ge(Li) detectors. From the EC decays of {sup 255}Md and {sup 256}Md, levels in {sup 255}Fm and {sup 256}Fm were deduced. Favored {alpha} decay of {sup 255}Md was found to populate the 7/2{sup -}[514] single-particle state in {sup 251}Es, thus establishing the 7/2{sup -}[514] as the {sup 255}Md ground state. Several {gamma} rays were observed in the {sup 256}Md {alpha}-{gamma} coincidence spectrum. {sup 256}Fm is the heaviest nucleus in which excited intrinsic states have been identified. (c) 2000 The American Physical Society.

  2. Developing Improved MD Codes for Understanding Processive Cellulases

    SciTech Connect

    Crowley, M. F.; Uberbacher, E. C.; Brooks III, C. L.; Walker, R.C.; Nimlos, M. R.; Himmel, M. E.

    2008-01-01

    The mechanism of action of cellulose-degrading enzymes is illuminated through a multidisciplinary collaboration that uses molecular dynamics (MD) simulations and expands the capabilities of MD codes to allow simulations of enzymes and substrates on petascale computational facilities. There is a class of glycoside hydrolase enzymes called cellulases that are thought to decrystallize and processively depolymerize cellulose using biochemical processes that are largely not understood. Understanding the mechanisms involved and improving the efficiency of this hydrolysis process through computational models and protein engineering presents a compelling grand challenge. A detailed understanding of cellulose structure, dynamics and enzyme function at the molecular level is required to direct protein engineers to the right modifications or to understand if natural thermodynamic or kinetic limits are in play. Much can be learned about processivity by conducting carefully designed molecular dynamics (MD) simulations of the binding and catalytic domains of cellulases with various substrate configurations, solvation models and thermodynamic protocols. Most of these numerical experiments, however, will require significant modification of existing code and algorithms in order to efficiently use current (terascale) and future (petascale) hardware to the degree of parallelism necessary to simulate a system of the size proposed here. This work will develop MD codes that can efficiently use terascale and petascale systems, not just for simple classical MD simulations, but also for more advanced methods, including umbrella sampling with complex restraints and reaction coordinates, transition path sampling, steered molecular dynamics, and quantum mechanical/molecular mechanical simulations of systems the size of cellulose degrading enzymes acting on cellulose.

  3. Apple fruit copper amine oxidase isoforms: peroxisomal MdAO1 prefers diamines as substrates, whereas extracellular MdAO2 exclusively utilizes monoamines.

    PubMed

    Zarei, Adel; Trobacher, Christopher P; Cooke, Alison R; Meyers, Ashley J; Hall, J Christopher; Shelp, Barry J

    2015-01-01

    4-Aminobutyrate (GABA) accumulates in apple fruit during controlled atmosphere storage. A potential source of GABA is the polyamine putrescine, which can be oxidized via copper-containing amine oxidase (CuAO), resulting in the production 4-aminobutanal/Δ(1)-pyrroline, with the consumption of O2 and release of H2O2 and ammonia. Five putative CuAO genes (MdAO genes) were cloned from apple (Malus domestica Borkh. cv. Empire) fruit, and the deduced amino acid sequences found to contain the active sites typically conserved in CuAOs. Genes encoding two of these enzymes, MdAO1 and MdAO2, were highly expressed in apple fruit and selected for further analysis. Amino acid sequence analysis predicted the presence of a C-terminal peroxisomal targeting signal 1 tripeptide in MdAO1 and an N-terminal signal peptide and N-glycosylation site in MdAO2. Transient expression of green fluorescent fusion proteins in Arabidopsis protoplasts or onion epidermal cells revealed a peroxisomal localization for MdAO1 and an extracellular localization for MdAO2. The enzymatic activities of purified recombinant MdAO1 and MdAO2 were measured continuously as H2O2 production using a coupled reaction. MdAO1 did not use monoamines or polyamines and displayed high catalytic efficiency for 1,3-diaminopropane, putrescine and cadaverine, whereas MdAO2 exclusively utilized aliphatic and aromatic monoamines, including 2-phenylethylamine and tyramine. Together, these results indicate that MdAO1 may contribute to GABA production via putrescine oxidation in the peroxisome of apple fruit under controlled atmosphere conditions. MdAO2 seems to be involved in deamination of 2-phenylethylamine, which is a step in the biosynthesis of 2-phenylethanol, a contributor to fruit flavor and flower fragrance.

  4. Apple fruit copper amine oxidase isoforms: peroxisomal MdAO1 prefers diamines as substrates, whereas extracellular MdAO2 exclusively utilizes monoamines.

    PubMed

    Zarei, Adel; Trobacher, Christopher P; Cooke, Alison R; Meyers, Ashley J; Hall, J Christopher; Shelp, Barry J

    2015-01-01

    4-Aminobutyrate (GABA) accumulates in apple fruit during controlled atmosphere storage. A potential source of GABA is the polyamine putrescine, which can be oxidized via copper-containing amine oxidase (CuAO), resulting in the production 4-aminobutanal/Δ(1)-pyrroline, with the consumption of O2 and release of H2O2 and ammonia. Five putative CuAO genes (MdAO genes) were cloned from apple (Malus domestica Borkh. cv. Empire) fruit, and the deduced amino acid sequences found to contain the active sites typically conserved in CuAOs. Genes encoding two of these enzymes, MdAO1 and MdAO2, were highly expressed in apple fruit and selected for further analysis. Amino acid sequence analysis predicted the presence of a C-terminal peroxisomal targeting signal 1 tripeptide in MdAO1 and an N-terminal signal peptide and N-glycosylation site in MdAO2. Transient expression of green fluorescent fusion proteins in Arabidopsis protoplasts or onion epidermal cells revealed a peroxisomal localization for MdAO1 and an extracellular localization for MdAO2. The enzymatic activities of purified recombinant MdAO1 and MdAO2 were measured continuously as H2O2 production using a coupled reaction. MdAO1 did not use monoamines or polyamines and displayed high catalytic efficiency for 1,3-diaminopropane, putrescine and cadaverine, whereas MdAO2 exclusively utilized aliphatic and aromatic monoamines, including 2-phenylethylamine and tyramine. Together, these results indicate that MdAO1 may contribute to GABA production via putrescine oxidation in the peroxisome of apple fruit under controlled atmosphere conditions. MdAO2 seems to be involved in deamination of 2-phenylethylamine, which is a step in the biosynthesis of 2-phenylethanol, a contributor to fruit flavor and flower fragrance. PMID:25378687

  5. Enhancing MD simulations of proteins using vague and combinatorics information

    NASA Astrophysics Data System (ADS)

    Dill, Ken

    We have developed MELD, a method that `melds' together replica-exchange molecular dynamics simulations with external information. Traditionally, accelerating MD simulations has only been possible by using information that is precise and correct. In contrast, MELD allows us to leverage information that is vague or corrupted. For example, we give generic instructives, such as `make a hydrophobic core', `make good secondary structures', or `search only compact structures'. Normally, such information implies a loss of ability to compute free energies and populations. But, MELD satisfies detailed balance. We show that it can fold small proteins much faster than brute-force MD can, that it gives reasonable populations, and that it can succeed in CASP, the blind protein-structure prediction event.

  6. Samuel Smith, M.D.: first American professor of psychiatry.

    PubMed

    Pinta, E R

    1994-04-01

    In 1847 Samuel M. Smith, M.D., was appointed professor of medical jurisprudence and insanity at the Willoughby Medical College of Columbus, Ohio, making him the first person to chair a department of psychiatry at an American medical school. Using materials from newspaper reports, records of the medical school and state medical society, and other sources, the author presents a biographical sketch of this pioneer educator. Dr. Smith received his first practical experience in psychiatry as an assistant to William M. Awl, M.D., one of the 13 founders of the American Psychiatric Association. Over the course of his career, he held many prominent positions, including lecturer and dean at the medical school, president of the Ohio State Medical Society, and surgeon general of Ohio during the Civil War.

  7. 78 FR 38001 - Special Local Regulations; Marine Events, Breton Bay; St. Mary's County, Leonardtown, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-25

    ...; St. Mary's County, Leonardtown, MD'' in the Federal Register (78 FR 21864). The rulemaking concerned... along longitude 076 38'30'' W, located at Leonardtown, MD. The regulations were needed to...

  8. Perryman Substation. Perryman, Hareford Co., MD. Sec. 1201, MP 69.30 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Perryman Substation. Perryman, Hareford Co., MD. Sec. 1201, MP 69.30 - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  9. Gunpowder Substation. Harewood, Baltimore Co., MD. Sec. 1201, MP 80.00. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Gunpowder Substation. Harewood, Baltimore Co., MD. Sec. 1201, MP 80.00. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  10. Orangeville Yards. Baltimore, Baltimore City, MD. Sec. 1201, MP 91.00. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Orangeville Yards. Baltimore, Baltimore City, MD. Sec. 1201, MP 91.00. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  11. Fulton Avenue Bridge/Tunnel. Baltimore, Baltimore City, MD. Sec. 1201, MP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Fulton Avenue Bridge/Tunnel. Baltimore, Baltimore City, MD. Sec. 1201, MP 97.56. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  12. North Point Substation. Rosedale, Baltimore Co., MD. Sec. 1201, 90.00. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North Point Substation. Rosedale, Baltimore Co., MD. Sec. 1201, 90.00. - Northeast Railroad Corridor, Amtrak route between District of Columbia/Maryland state line & Maryland/Delaware state line, Baltimore, Independent City, MD

  13. Hybrid MC/MD Method For High Entropy Alloy

    NASA Astrophysics Data System (ADS)

    Feng, Bojun; Widom, Michael

    High entropy alloys (HEA) are materials that contain multiple components of elements consisting of a single solid solution phase which could make the entropy of mixing very high. From recent investigations, HEA's possess promising properties such as strength at high temperature, tensile strength, thermal stability and corrosion resistance. In this talk, a hybrid Molecular Dynamics (MD)/Monte Carlo (MC) simulation method is introduced to the computational analysis of HEA, treating atomic displacement by MD as well as swapping atomic species by MC. This method efficiently models the phase separation and short range order by swapping between different types of atoms, while structural deviation from the perfect lattice sites of atoms is equilibrated quickly by MD. We apply this method to HfNbTaZr HEA modeled using an embedded-atom potential. The result gives a strong phase separation of Hf-Zr and Nb-Ta pairs shown by the pair correlation function. Diffuse scattering patterns are predicted and compared to experiments. Doe Grant No. DE-SC0014506.

  14. Structure and Properties of HELICAL CARBON NANOTUBES through MD Simulations

    NASA Astrophysics Data System (ADS)

    Dahiya, Akshay; Verma, Deepti; Gupta, Shakti S.

    Helical Carbon Nanotubes (HCNTs) are coiled 3-valent carbon networks which represent pure carbon helix. Here we study the geometries of two classes: hexagonal helix containing purely polyhex networks and the second class with 5-and 7-membered rings besides hexagons. We followed a model of hexagonal, single wall HCNTs, and determined their relaxed configuration using MD simulations based on Tersoff potential. A race-track like structure is observed in the cross-section of HCNTs upon minimization. For generating class two helix, the adjacency matrix eigenvector's (AME) method is applied which utilizes 3-coordinated tiling of the plane by 5-,6-,and 7-membered ring for the construction of helical structures. The application of the AME method to torusenes is crucial for class two helix generation as it is based on an appropriate choice of bi-lobial eigenvectors triplet which can be selected on the basis of their nodal properties as verified here. After 3-D transformations the final structure was obtained with the help of MM3-potential based MD simulations on Tinker commercial code. The spring constants of HCNTs are computed through MD simulations.

  15. 76 FR 71369 - Robert G. Crummie, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-17

    ... authority under the laws of the state in which he does business. See Scott Sandarg, D.M.D., 74 FR 17528 (DEA 2009); David W. Wang, M.D., 72 FR 54297 (DEA 2007); Sheran Arden Yeates, M.D., 71 FR 39130 (DEA 2006); Dominick A. Ricci, M.D., 58 FR 51104 (DEA 1993); Bobby Watts M.D., 53 FR 11919 (DEA 1988). In the...

  16. 77 FR 67669 - Wayne D. Longmore, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-13

    .... Edwards, M.D., 75 FR 49,991 (DEA 2010); Joseph Baumstarck, M.D., 74 FR 17,525 (DEA 2009). In this case... Baumstarck, M.D., 74 FR at 17,527 (DEA 2009). Thus, because there is no dispute that the Respondent lacks... cancellation); William R. Lockridge, M.D., 71 FR 77,791, 77,797 (DEA 2006) (interpreting 21 CFR 1301.52(a)...

  17. 76 FR 71370 - Silviu Ziscovici, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-17

    ... the state in which he does business. See Scott Sandarg, D.M.D., 74 FR 17,528 (DEA 2009); David W. Wang, M.D., 72 FR 54,297 (DEA 2007); Sheran ] Arden Yeates, M.D., 71 FR 39,130 (DEA 2006); Dominick A. Ricci, M.D., 58 FR 51,104 (DEA 1993); Bobby Watts M.D., 53 Fed. Reg. 11,919 (DEA 1988)....

  18. 78 FR 7813 - Sanjay Trivedi, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-04

    ... authority''); Beverley P. Edwards, M.D., 75 FR 49,991 (DEA 2010); Joseph Baumstarck, M.D., 74 FR 17,525 (DEA... Florida. Consequently, his DEA registration must be revoked. See Joseph Baumstarck, M.D., 74 FR 17,525, 17... Coast Specialty Pharmacy, 76 FR 66,965 (DEA 2011); Roy Chi Lung, M.D., 74 FR 20,346 (DEA 2009);...

  19. 77 FR 7182 - Scott W. Houghton, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-10

    ... practitioner who lacks .'' Roy Chi Lung, 74 FR 20346, 20347 (2009); Scott Sandarg, D.M.D., 74 FR 17528, 174529 (2009); John B. Freitas, D.O., 74 FR 17524, 17525 (2009); Roger A. Rodriguez, M.D., 70 FR 33206, 33207 (2005); Stephen J. Graham, M.D., 69 FR 11661 (2004); Dominick A. Ricci, M.D., 58 FR 51104...

  20. 78 FR 19009 - Gary Alfred Shearer, M.D.; Decision And Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... (2000); Jesus R. Juarez, M.D., 62 FR 14945 (1997); see also Philip E. Kirk, M.D., 48 FR 32887 (1983... proceeding under section 304, 21 U.S.C. 824, of the CSA.'' Zhiwei Lin, 77 FR 18862, 18864 (2012) (citing... Kentucky, not Florida.) \\16\\ See Michael G. Dolin, M.D., 65 FR 5661 (2000); see also Philip E. Kirk,...

  1. 77 FR 67673 - Fernando Valle, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-13

    ... not required. See Jesus R. Juarez, M.D., 62 FR 14945 (1997); Dominick A. Ricci, M.D., 58 FR 51104... DEA registration. Serenity Caf , 77 FR 35027, 35028 (2012); David W. Wang, 72 FR 54297, 54298 (2007); Sheran Arden Yeates, 71 FR 39130, 39131 (2006); Dominick A. Ricci, M.D., 58 FR 51104 (1993); Bobby...

  2. 77 FR 67671 - Larry Elbert Perry, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-13

    ... Enforcement Administration Larry Elbert Perry, M.D.; Decision and Order On July 2, 2012, Chief Administrative... Elbert Perry, M.D., be, and it hereby is, revoked. I further order that any pending application of Larry Elbert Perry, M.D., to renew or modify his registration, be, and it hereby is, denied. This Order...

  3. The Application Research of MD5 Encryption Algorithm in DCT Digital Watermarking

    NASA Astrophysics Data System (ADS)

    Xijin, Wang; Linxiu, Fan

    This article did the preliminary study of the application of algorithm for MD5 in the digital watermark. It proposed that copyright information will be encrypted using an algorithm MD5, and made rules for the second value image watermarks, through DCT algorithm that embeds an image by the carrier. The extraction algorithms can pick up the watermark and restore MD5 code.

  4. 76 FR 60889 - Stephen L. Reitman, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-30

    ... Enforcement Administration Stephen L. Reitman, M.D.; Decision and Order On July 20, 2010, Administrative Law... January 19, 2011. In re Stephen Lee Reitman, M.D., Decision at 1 (Cal. Med. Bd. Dec. 20, 2010). I take... substances properly if entrusted with a DEA registration.''' Id. (quoting Leonardo v. Lopez, M.D., 54...

  5. Crystal structure of soluble MD-1 and its interaction with lipid IVa

    SciTech Connect

    Yoon, Sung-il; Hong, Minsun; Han, Gye Won; Wilson, Ian A.

    2010-07-22

    Lipopolysaccharide (LPS) of Gram-negative bacteria is a common pathogen-associated molecular pattern (PAMP) that induces potent innate immune responses. The host immune response against LPS is triggered by myeloid differentiation factor 2 (MD-2) in association with Toll-like receptor 4 (TLR4) on the cell surface. The MD-2/TLR4-mediated LPS response is regulated by the evolutionarily related complex of MD-1 and Toll-like receptor homolog RP105. Here, we report crystallographic and biophysical data that demonstrate a previously unidentified direct interaction of MD-1 with LPS. The crystal structure of chicken MD-1 (cMD-1) at 2.0 {angstrom} resolution exhibits a {beta}-cup-like fold, similar to MD-2, that encloses a hydrophobic cavity between the two {beta}-sheets. A lipid-like moiety was observed inside the cavity, suggesting the possibility of a direct MD-1/LPS interaction. LPS was subsequently identified as an MD-1 ligand by native gel electrophoresis and gel filtration analyses. The crystal structure of cMD-1 with lipid IVa, an LPS precursor, at 2.4 {angstrom} resolution revealed that the lipid inserts into the deep hydrophobic cavity of the {beta}-cup-like structure, but with some important differences compared with MD-2. These findings suggest that soluble MD-1 alone, in addition to its complex with RP105, can regulate host LPS sensitivity.

  6. Validity of the Medical College Admission Test for Predicting MD-PhD Student Outcomes

    ERIC Educational Resources Information Center

    Bills, James L.; VanHouten, Jacob; Grundy, Michelle M.; Chalkley, Roger; Dermody, Terence S.

    2016-01-01

    The Medical College Admission Test (MCAT) is a quantitative metric used by MD and MD-PhD programs to evaluate applicants for admission. This study assessed the validity of the MCAT in predicting training performance measures and career outcomes for MD-PhD students at a single institution. The study population consisted of 153 graduates of the…

  7. 75 FR 52461 - Drawbridge Operation Regulation; Pocomoke River, Snow Hill, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-26

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulation; Pocomoke River, Snow Hill, MD AGENCY... the S12 ] Bridge across the Pocomoke River, mile 29.9, at Snow Hill, MD. The deviation restricts the... hours advance notice is given. The S12 Bridge across Pocomoke River, mile 29.9 at Snow Hill MD, has...

  8. The Class of 1989 and post-MD training

    PubMed Central

    Ryten, E; Thurber, A D; Buske, L

    1998-01-01

    BACKGROUND: "The Class of 1989" is a longitudinal study of 1722 people who were awarded an MD degree by a Canadian university in 1989. This paper reports on the details of their post-MD training up to spring 1996. METHODS: Several medical professional and educational associations in Canada and the United States provided year-by-year information on field and location of post-MD training, certification achieved, whether in practice and location of practice through to spring 1996. Information from all sources was linked to a list of 1989 medical school graduates. RESULTS: Of the 1722 graduates 57 (3.3%) never entered post-MD training in Canada; 147 (8.5%) did 1 or more years of training in the United States. A total of 222 graduates (12.9%) took a break of at least 1 year from training, and 301 (17.5%) changed their choice of field or specialty after starting training. Substantial numbers took 1 or more years longer to complete training than would be expected based on the prescribed length of the training program chosen. The field or specialty choices of the cohort produced a generalist:specialist ratio of 58:42. The final numbers in several fields depended heavily on trainees changing their initial career choice. INTERPRETATION: The data point out widely differing and often very long lead times from start to completion of training. Since 1993, changes to licensure requirements have reduced opportunities for recent graduating cohorts to delay final career choices, take a break in training, prolong training or change initial career choices. Rigidities in the post-1993 training environment point to the emergence of a number of serious problems, such as dissatisfaction and high anxiety levels among residents, licensing authorities being faced with people who have not completed a training program to certification, and insufficient provision of positions for post-MD training because of underestimates of the time needed to complete training programs. The insights gained

  9. Atomic Mass and Nuclear Binding Energy for Md-281 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-281 (Mendelevium, atomic number Z = 101, mass number A = 281).

  10. Atomic Mass and Nuclear Binding Energy for Md-289 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-289 (Mendelevium, atomic number Z = 101, mass number A = 289).

  11. Atomic Mass and Nuclear Binding Energy for Md-282 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-282 (Mendelevium, atomic number Z = 101, mass number A = 282).

  12. Atomic Mass and Nuclear Binding Energy for Md-330 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-330 (Mendelevium, atomic number Z = 101, mass number A = 330).

  13. Atomic Mass and Nuclear Binding Energy for Md-299 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-299 (Mendelevium, atomic number Z = 101, mass number A = 299).

  14. Atomic Mass and Nuclear Binding Energy for Md-318 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-318 (Mendelevium, atomic number Z = 101, mass number A = 318).

  15. Atomic Mass and Nuclear Binding Energy for Md-328 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-328 (Mendelevium, atomic number Z = 101, mass number A = 328).

  16. Atomic Mass and Nuclear Binding Energy for Md-263 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-263 (Mendelevium, atomic number Z = 101, mass number A = 263).

  17. Atomic Mass and Nuclear Binding Energy for Md-286 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-286 (Mendelevium, atomic number Z = 101, mass number A = 286).

  18. Atomic Mass and Nuclear Binding Energy for Md-324 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-324 (Mendelevium, atomic number Z = 101, mass number A = 324).

  19. Atomic Mass and Nuclear Binding Energy for Md-331 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-331 (Mendelevium, atomic number Z = 101, mass number A = 331).

  20. Atomic Mass and Nuclear Binding Energy for Md-279 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-279 (Mendelevium, atomic number Z = 101, mass number A = 279).

  1. Atomic Mass and Nuclear Binding Energy for Md-334 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-334 (Mendelevium, atomic number Z = 101, mass number A = 334).

  2. Atomic Mass and Nuclear Binding Energy for Md-326 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-326 (Mendelevium, atomic number Z = 101, mass number A = 326).

  3. Atomic Mass and Nuclear Binding Energy for Md-297 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-297 (Mendelevium, atomic number Z = 101, mass number A = 297).

  4. Atomic Mass and Nuclear Binding Energy for Md-339 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-339 (Mendelevium, atomic number Z = 101, mass number A = 339).

  5. Atomic Mass and Nuclear Binding Energy for Md-315 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-315 (Mendelevium, atomic number Z = 101, mass number A = 315).

  6. Atomic Mass and Nuclear Binding Energy for Md-268 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-268 (Mendelevium, atomic number Z = 101, mass number A = 268).

  7. Atomic Mass and Nuclear Binding Energy for Md-335 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-335 (Mendelevium, atomic number Z = 101, mass number A = 335).

  8. Atomic Mass and Nuclear Binding Energy for Md-272 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-272 (Mendelevium, atomic number Z = 101, mass number A = 272).

  9. Atomic Mass and Nuclear Binding Energy for Md-329 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-329 (Mendelevium, atomic number Z = 101, mass number A = 329).

  10. Atomic Mass and Nuclear Binding Energy for Md-336 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-336 (Mendelevium, atomic number Z = 101, mass number A = 336).

  11. Atomic Mass and Nuclear Binding Energy for Md-307 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-307 (Mendelevium, atomic number Z = 101, mass number A = 307).

  12. Atomic Mass and Nuclear Binding Energy for Md-273 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-273 (Mendelevium, atomic number Z = 101, mass number A = 273).

  13. Atomic Mass and Nuclear Binding Energy for Md-308 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-308 (Mendelevium, atomic number Z = 101, mass number A = 308).

  14. Atomic Mass and Nuclear Binding Energy for Md-327 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-327 (Mendelevium, atomic number Z = 101, mass number A = 327).

  15. Atomic Mass and Nuclear Binding Energy for Md-341 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-341 (Mendelevium, atomic number Z = 101, mass number A = 341).

  16. Atomic Mass and Nuclear Binding Energy for Md-266 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-266 (Mendelevium, atomic number Z = 101, mass number A = 266).

  17. Atomic Mass and Nuclear Binding Energy for Md-274 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-274 (Mendelevium, atomic number Z = 101, mass number A = 274).

  18. Atomic Mass and Nuclear Binding Energy for Md-338 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-338 (Mendelevium, atomic number Z = 101, mass number A = 338).

  19. Atomic Mass and Nuclear Binding Energy for Md-291 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-291 (Mendelevium, atomic number Z = 101, mass number A = 291).

  20. Atomic Mass and Nuclear Binding Energy for Md-321 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-321 (Mendelevium, atomic number Z = 101, mass number A = 321).

  1. Atomic Mass and Nuclear Binding Energy for Md-319 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-319 (Mendelevium, atomic number Z = 101, mass number A = 319).

  2. Atomic Mass and Nuclear Binding Energy for Md-267 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-267 (Mendelevium, atomic number Z = 101, mass number A = 267).

  3. Atomic Mass and Nuclear Binding Energy for Md-325 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-325 (Mendelevium, atomic number Z = 101, mass number A = 325).

  4. Atomic Mass and Nuclear Binding Energy for Md-302 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-302 (Mendelevium, atomic number Z = 101, mass number A = 302).

  5. Atomic Mass and Nuclear Binding Energy for Md-292 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-292 (Mendelevium, atomic number Z = 101, mass number A = 292).

  6. Atomic Mass and Nuclear Binding Energy for Md-304 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-304 (Mendelevium, atomic number Z = 101, mass number A = 304).

  7. Atomic Mass and Nuclear Binding Energy for Md-288 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-288 (Mendelevium, atomic number Z = 101, mass number A = 288).

  8. Atomic Mass and Nuclear Binding Energy for Md-254 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-254 (Mendelevium, atomic number Z = 101, mass number A = 254).

  9. Atomic Mass and Nuclear Binding Energy for Md-261 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-261 (Mendelevium, atomic number Z = 101, mass number A = 261).

  10. Atomic Mass and Nuclear Binding Energy for Md-275 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-275 (Mendelevium, atomic number Z = 101, mass number A = 275).

  11. Atomic Mass and Nuclear Binding Energy for Md-337 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-337 (Mendelevium, atomic number Z = 101, mass number A = 337).

  12. Atomic Mass and Nuclear Binding Energy for Md-320 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-320 (Mendelevium, atomic number Z = 101, mass number A = 320).

  13. Atomic Mass and Nuclear Binding Energy for Md-285 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-285 (Mendelevium, atomic number Z = 101, mass number A = 285).

  14. Atomic Mass and Nuclear Binding Energy for Md-284 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-284 (Mendelevium, atomic number Z = 101, mass number A = 284).

  15. Atomic Mass and Nuclear Binding Energy for Md-332 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-332 (Mendelevium, atomic number Z = 101, mass number A = 332).

  16. Atomic Mass and Nuclear Binding Energy for Md-290 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-290 (Mendelevium, atomic number Z = 101, mass number A = 290).

  17. Atomic Mass and Nuclear Binding Energy for Md-312 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-312 (Mendelevium, atomic number Z = 101, mass number A = 312).

  18. Atomic Mass and Nuclear Binding Energy for Md-296 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-296 (Mendelevium, atomic number Z = 101, mass number A = 296).

  19. Atomic Mass and Nuclear Binding Energy for Md-253 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-253 (Mendelevium, atomic number Z = 101, mass number A = 253).

  20. Atomic Mass and Nuclear Binding Energy for Md-314 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-314 (Mendelevium, atomic number Z = 101, mass number A = 314).

  1. Atomic Mass and Nuclear Binding Energy for Md-301 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-301 (Mendelevium, atomic number Z = 101, mass number A = 301).

  2. Atomic Mass and Nuclear Binding Energy for Md-317 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-317 (Mendelevium, atomic number Z = 101, mass number A = 317).

  3. Atomic Mass and Nuclear Binding Energy for Md-271 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-271 (Mendelevium, atomic number Z = 101, mass number A = 271).

  4. Atomic Mass and Nuclear Binding Energy for Md-313 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-313 (Mendelevium, atomic number Z = 101, mass number A = 313).

  5. Atomic Mass and Nuclear Binding Energy for Md-293 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-293 (Mendelevium, atomic number Z = 101, mass number A = 293).

  6. Atomic Mass and Nuclear Binding Energy for Md-277 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-277 (Mendelevium, atomic number Z = 101, mass number A = 277).

  7. Atomic Mass and Nuclear Binding Energy for Md-298 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-298 (Mendelevium, atomic number Z = 101, mass number A = 298).

  8. Atomic Mass and Nuclear Binding Energy for Md-287 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-287 (Mendelevium, atomic number Z = 101, mass number A = 287).

  9. Atomic Mass and Nuclear Binding Energy for Md-262 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-262 (Mendelevium, atomic number Z = 101, mass number A = 262).

  10. Atomic Mass and Nuclear Binding Energy for Md-280 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-280 (Mendelevium, atomic number Z = 101, mass number A = 280).

  11. Atomic Mass and Nuclear Binding Energy for Md-270 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-270 (Mendelevium, atomic number Z = 101, mass number A = 270).

  12. Atomic Mass and Nuclear Binding Energy for Md-260 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-260 (Mendelevium, atomic number Z = 101, mass number A = 260).

  13. Atomic Mass and Nuclear Binding Energy for Md-303 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-303 (Mendelevium, atomic number Z = 101, mass number A = 303).

  14. Atomic Mass and Nuclear Binding Energy for Md-316 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-316 (Mendelevium, atomic number Z = 101, mass number A = 316).

  15. Atomic Mass and Nuclear Binding Energy for Md-283 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-283 (Mendelevium, atomic number Z = 101, mass number A = 283).

  16. Atomic Mass and Nuclear Binding Energy for Md-264 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-264 (Mendelevium, atomic number Z = 101, mass number A = 264).

  17. Atomic Mass and Nuclear Binding Energy for Md-278 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-278 (Mendelevium, atomic number Z = 101, mass number A = 278).

  18. Atomic Mass and Nuclear Binding Energy for Md-276 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-276 (Mendelevium, atomic number Z = 101, mass number A = 276).

  19. Atomic Mass and Nuclear Binding Energy for Md-259 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-259 (Mendelevium, atomic number Z = 101, mass number A = 259).

  20. Atomic Mass and Nuclear Binding Energy for Md-306 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-306 (Mendelevium, atomic number Z = 101, mass number A = 306).

  1. Atomic Mass and Nuclear Binding Energy for Md-309 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-309 (Mendelevium, atomic number Z = 101, mass number A = 309).

  2. Atomic Mass and Nuclear Binding Energy for Md-333 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-333 (Mendelevium, atomic number Z = 101, mass number A = 333).

  3. Atomic Mass and Nuclear Binding Energy for Md-305 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-305 (Mendelevium, atomic number Z = 101, mass number A = 305).

  4. Atomic Mass and Nuclear Binding Energy for Md-323 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-323 (Mendelevium, atomic number Z = 101, mass number A = 323).

  5. Atomic Mass and Nuclear Binding Energy for Md-310 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-310 (Mendelevium, atomic number Z = 101, mass number A = 310).

  6. Atomic Mass and Nuclear Binding Energy for Md-300 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-300 (Mendelevium, atomic number Z = 101, mass number A = 300).

  7. Atomic Mass and Nuclear Binding Energy for Md-269 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-269 (Mendelevium, atomic number Z = 101, mass number A = 269).

  8. Atomic Mass and Nuclear Binding Energy for Md-295 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-295 (Mendelevium, atomic number Z = 101, mass number A = 295).

  9. Atomic Mass and Nuclear Binding Energy for Md-265 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-265 (Mendelevium, atomic number Z = 101, mass number A = 265).

  10. Atomic Mass and Nuclear Binding Energy for Md-294 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-294 (Mendelevium, atomic number Z = 101, mass number A = 294).

  11. Atomic Mass and Nuclear Binding Energy for Md-311 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-311 (Mendelevium, atomic number Z = 101, mass number A = 311).

  12. Atomic Mass and Nuclear Binding Energy for Md-322 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-322 (Mendelevium, atomic number Z = 101, mass number A = 322).

  13. LifeBridge new publication: Md.MD. Listing of 1,200 physicians a major part of consumer-type magazine.

    PubMed

    Botvin, Judith D

    2004-01-01

    LifeBridge Health, Baltimore, created a directory of its physicians, which developed into a 100-page consumer magazine titled Md.MD A Directory of the Region's Finest Physicians. Written and designed in-house, the premier edition paid for itself, thanks to publishing partner, Alter Communications. The premier edition was mailed to 50,000 Baltimore area homes last September. PMID:15022542

  14. Full-Scale Crash Test of an MD-500 Helicopter

    NASA Technical Reports Server (NTRS)

    Littell, Justin

    2011-01-01

    A full-scale crash test was successfully conducted in March 2010 of an MD-500 helicopter at NASA Langley Research Center s Landing and Impact Research Facility. The reasons for conducting this test were threefold: 1 To generate data to be used with finite element computer modeling efforts, 2 To study the crashworthiness features typically associated with a small representative helicopter, and 3 To compare aircraft response to data collected from a previously conducted MD-500 crash test, which included an externally deployable energy absorbing (DEA) concept. Instrumentation on the airframe included accelerometers on various structural components of the airframe; and strain gages on keel beams, skid gear and portions of the skin. Three Anthropomorphic Test Devices and a specialized Human Surrogate Torso Model were also onboard to collect occupant loads for evaluation with common injury risk criteria. This paper presents background and results from this crash test conducted without the DEA concept. These results showed accelerations of approximately 30 to 50 g on the airframe at various locations, little energy attenuation through the airframe, and moderate to high probability of occupant injury for a variety of injury criteria.

  15. Harvey Cushing, M.D., in his world.

    PubMed

    Hart, Curtis W

    2014-12-01

    Harvey Cushing, M.D. (1869-1939), is the acknowledged father of the discipline of neurosurgery who inspired others to join him in this new field. He was a prolific researcher in the area of human growth disturbances. And he was among the most literary of doctors having won the Pulitzer Prize for his two-volume biography of his mentor and teacher William Osler, M.D. A driven man, he both inspired and intimidated others. This essay explores Cushing's character and background along with his relationship to Osler. It seeks to understand why and how he may be considered a great figure in spite and because of his demanding and often problematic character. It further seeks to place Cushing in the context of the transition of American society and American medicine in the latter decades of the nineteenth and early decades of the twentieth century. Portions of this essay were originally delivered as part of a Grand Rounds presentation for the Department of Neurosurgery at the New York Presbyterian Hospital Weill Cornell Center. PMID:25064311

  16. Harvey Cushing, M.D., in his world.

    PubMed

    Hart, Curtis W

    2014-12-01

    Harvey Cushing, M.D. (1869-1939), is the acknowledged father of the discipline of neurosurgery who inspired others to join him in this new field. He was a prolific researcher in the area of human growth disturbances. And he was among the most literary of doctors having won the Pulitzer Prize for his two-volume biography of his mentor and teacher William Osler, M.D. A driven man, he both inspired and intimidated others. This essay explores Cushing's character and background along with his relationship to Osler. It seeks to understand why and how he may be considered a great figure in spite and because of his demanding and often problematic character. It further seeks to place Cushing in the context of the transition of American society and American medicine in the latter decades of the nineteenth and early decades of the twentieth century. Portions of this essay were originally delivered as part of a Grand Rounds presentation for the Department of Neurosurgery at the New York Presbyterian Hospital Weill Cornell Center.

  17. [Professor Frantisek Por MD and Professor Robert Klopstock MD, students at Budapest and Prague Faculties of Medicine].

    PubMed

    Mydlík, M; Derzsiová, K

    2010-11-01

    Professor Frantisek Por MD and Professor Robert Klopstock MD were contemporaries, both born in 1899, one in Zvolen, the other in Dombovar, at the time of Austro-Hungarian Monarchy. Prof. Por attended the Faculty of Medicine in Budapest from 1918 to 1920, and Prof. Klopstock studied at the same place between 1917 and 1919. From 1920 until graduation on 6th February 1926, Prof. Por continued his studies at the German Faculty of Medicine, Charles University in Prague. Prof. Klopstock had to interrupt his studies in Budapest due to pulmonary tuberculosis; he received treatment at Tatranske Matliare where he befriended Franz Kafka. Later, upon Kafka's encouragement, he changed institutions and continued his studies at the German Faculty of Medicine, Charles University in Prague, where he graduated the first great go. It is very likely that, during their studies in Budapest and Prague, both professors met repeatedly, even though their life paths later separated. Following his graduation, Prof. Por practiced as an internist in Prague, later in Slovakia, and from 1945 in Kosice. In 1961, he was awarded the title of university professor of internal medicine at the Faculty of Medicine, Pavol Jozef Safarik University in Kosice, where he practiced until his death in 1980. Prof. Klopstock continued his studies in Kiel and Berlin. After his graduation in 1933, he practiced in Berlin as a surgeon and in 1938 left for USA. In 1962, he was awarded the title of university professor of pulmonary surgery in NewYork, where he died in 1972.

  18. 78 FR 19012 - Pawan Kumar Jain, M.D.; Decision And Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ...., 65 FR 5,661 (DEA 2000); Jesus R. Juarez, M.D., 62 FR 14,945 (DEA 1997). Accordingly, both the plain.... Edwards, M.D., 75 FR 49,991 (DEA 2010); Joseph Baumstarck, M.D., 74 FR 17,525 (DEA 2009). In this case... practice medicine, his DEA registration must be revoked. See 21 U.S.C. 824(a)(3); Edwards, 75 FR...

  19. Fred Loe, MD, and the history of trachoma.

    PubMed

    Feibel, Robert M

    2011-04-01

    Trachoma has been one of the most blinding diseases in the history of ophthalmology. From its initial description in antiquity until the late 1930s, no specific treatment or effective cure had been known, and the only expedient had been to destroy the diseased tissue containing the infectious agent, rendering the disease inactive. Virtually all medical, mechanical, and surgical treatments were unsatisfactory, with cure rates of approximately 20%. Therapy for trachoma had barely advanced from the measures used by the ancient Egyptian, Greek, and Roman physicians. All prior therapies became obsolete in 1938 when Fred Loe, MD, working on an American Indian reservation, introduced sulfanilamide as a treatment of trachoma, achieving a 90% cure rate. One of the most unusual aspects of Loe's career was that he had no formal training in ophthalmology and was completely self-taught as an ophthalmologist. PMID:21482877

  20. A workshop on leadership for MD/PhD students

    PubMed Central

    Ciampa, Erin j.; Hunt, Aubrey A.; Arneson, Kyle O.; Mordes, Daniel A.; Oldham, William M.; Vin Woo, Kel; Owens, David A.; Cannon, Mark D.; Dermody, Terence S.

    2011-01-01

    Success in academic medicine requires scientific and clinical aptitude and the ability to lead a team effectively. Although combined MD/PhD training programs invest considerably in the former, they often do not provide structured educational opportunities in leadership, especially as applied to investigative medicine. To fill a critical knowledge gap in physician-scientist training, the Vanderbilt Medical Scientist Training Program (MSTP) developed a biennial two-day workshop in investigative leadership. MSTP students worked in partnership with content experts to develop a case-based curriculum and deliver the material. In its initial three offerings in 2006, 2008, and 2010, the workshop was judged by MSTP student attendees to be highly effective. The Vanderbilt MSTP Leadership Workshop offers a blueprint for collaborative student-faculty interactions in curriculum design and a new educational modality for physician-scientist training. PMID:21841905

  1. RADIATION PRESSURE DETECTION AND DENSITY ESTIMATE FOR 2011 MD

    SciTech Connect

    Micheli, Marco; Tholen, David J.; Elliott, Garrett T. E-mail: tholen@ifa.hawaii.edu

    2014-06-10

    We present our astrometric observations of the small near-Earth object 2011 MD (H ∼ 28.0), obtained after its very close fly-by to Earth in 2011 June. Our set of observations extends the observational arc to 73 days, and, together with the published astrometry obtained around the Earth fly-by, allows a direct detection of the effect of radiation pressure on the object, with a confidence of 5σ. The detection can be used to put constraints on the density of the object, pointing to either an unexpectedly low value of ρ=(640±330)kg m{sup −3} (68% confidence interval) if we assume a typical probability distribution for the unknown albedo, or to an unusually high reflectivity of its surface. This result may have important implications both in terms of impact hazard from small objects and in light of a possible retrieval of this target.

  2. Thermal Sensitivity of MD Hematite: Implication for Magnetic Anomalies

    NASA Technical Reports Server (NTRS)

    Kletetschka, Gunther; Wasilewski, Peter J.; Taylor, Patrick T.

    1999-01-01

    Magnetic remanence of crustal rocks can reside in three common rock-forming magnetic minerals: magnetite, pyrrhotite, and hematite. Thermoremanent magnetization (TRM) of magnetite and pyrrhotite is carried mostly by single domain (SD) grains. The TRM of hematite grains, however, is carried mostly by multidomain (NM) grains. This characteristic is illustrated by TRM acquisition curves for hematite of variable grainsizes. The transition between truly NM behavior and tendency towards SD behavior his been established between hematite grainsizes of 0. 1 and 0.05 mm. Coarse grainsize of lower crustal rocks and the large sensitivity of MD hematite grains to acquire TRM indicates that hematite could be a significant contributor to long-wavelength magnetic anomalies.

  3. Emily Dickinson's ophthalmic consultation with Henry Willard Williams, MD.

    PubMed

    Blanchard, Donald L

    2012-12-01

    Emily Dickinson is one of America's premier poets of the 19th century. Henry Willard Williams, MD, was one of the very first physicians to limit his practice to ophthalmology and was the established leader in his field in Boston, Massachusetts. They met during the time of the Civil War, when Emily consulted him about her ophthalmic disorder. No records of the diagnosis survive. Photophobia, aching eyes, and a restriction in her ability to work up close were her main symptoms. Iritis, exotropia, or psychiatric problems are the most frequent diagnoses offered to explain her difficulties. Rather than attempt a definitive conclusion, this article will offer an additional possibility that Dr Williams likely considered (ie, hysterical hyperaesthesia of the retina). This was a common diagnosis at that time, although it has currently faded from use.

  4. Plasticity and Failure in Nanocrystalline BCC Metals via MD Simulation

    SciTech Connect

    Rudd, R E

    2010-02-12

    Advances in the ability to generate extremely high pressures in dynamic experiments such as at the National Ignition Facility has motivated the need for special materials optimized for those conditions as well as ways to probe the response of these materials as they are deformed. We need to develop a much deeper understanding of the behavior of materials subjected to high pressure, especially the effect of rate at the extremely high rates encountered in those experiments. Here we use large-scale molecular dynamics (MD) simulations of the high-rate deformation of nanocrystalline tantalum at pressures less than 100 GPa to investigate the processes associated with plastic deformation for strains up to 100%. We focus on 3D polycrystalline systems with typical grain sizes of 10-20 nm. We also study a rapidly quenched liquid (amorphous solid) tantalum. We apply a constant volume (isochoric), constant temperature (isothermal) shear deformation over a range of strain rates, and compute the resulting stress-strain curves to large strains for both uniaxial and biaxial compression. We study the rate dependence and identify plastic deformation mechanisms. The identification of the mechanisms is facilitated through a novel technique that computes the local grain orientation, returning it as a quaternion for each atom. This analysis technique is robust and fast, and has been used to compute the orientations on the fly during our parallel MD simulations on supercomputers. We find both dislocation and twinning processes are important, and they interact in the weak strain hardening in these extremely fine-grained microstructures. We also present some results on void growth in nanocrystalline BCC metals under tension.

  5. MD-2 determinants of nickel and cobalt-mediated activation of human TLR4.

    PubMed

    Oblak, Alja; Pohar, Jelka; Jerala, Roman

    2015-01-01

    Recent findings unexpectedly revealed that human TLR4 can be directly activated by nickel ions. This activation is due to the coordination of nickel by a cluster of histidine residues on the ectodomain of human TLR4, which is absent in most other species. We aimed to elucidate the role of MD-2 in the molecular mechanism of TLR4/MD-2 activation by nickel, as nickel binding site on TLR4 is remote from MD-2, which directly binds the endotoxin as the main pathological activator of TLR4. We identified MD-2 and TLR4 mutants which abolished TLR4/MD-2 receptor activation by endotoxin but could nevertheless be significantly activated by nickel, which acts in synergy with LPS. Human TLR4/MD-2 was also activated by cobalt ions, while copper and cadmium were toxic in the tested concentration range. Activation of TLR4 by cobalt required MD-2 and was abolished by human TLR4 mutations of histidine residues at positions 456 and 458. We demonstrated that activation of TLR4 by nickel and cobalt ions can trigger both the MyD88-dependent and the -independent pathway. Based on our results we propose that predominantly hydrophobic interactions between MD-2 and TLR4 contribute to the stabilization of the TLR4/MD-2/metal ion complex in a conformation that enables activation.

  6. Characterization of an Autophagy-Related Gene MdATG8i from Apple.

    PubMed

    Wang, Ping; Sun, Xun; Jia, Xin; Wang, Na; Gong, Xiaoqing; Ma, Fengwang

    2016-01-01

    Nutrient deficiencies restrict apple (Malus sp.) tree growth and productivity in Northwest China. The process of autophagy, a conserved degradation pathway in eukaryotic cells, has important roles in nutrient-recycling and helps improve plant performance during periods of nutrient-starvation. Little is known about the functioning of autophagy-related genes (ATGs) in apple. In this study, one of the ATG8 gene family members MdATG8i was isolated from Malus domestica. MdATG8i has conserved putative tubulin binding sites and ATG7 interaction domains. A 1865-bp promoter region cloned from apple genome DNA was predicated to have cis-regulatory elements responsive to light, environmental stresses, and hormones. MdATG8i transcriptions were induced in response to leaf senescence, nitrogen depletion, and oxidative stress. At cellular level, MdATG8i protein was expressed in the nucleus and cytoplasm of onion epidermal cells. Yeast two-hybrid tests showed that MdATG8i could interact with MdATG7a and MdATG7b. In Arabidopsis, its heterologous expression was associated with enhanced vegetative growth, leaf senescence, and tolerance to nitrogen- and carbon-starvation. MdATG8i-overexpressing "Orin" apple callus lines also displayed improved tolerance to nutrient-limited conditions. Our results demonstrate that MdATG8i protein could function in autophagy in a conserved way, as a positive regulator in the response to nutrient-starvation. PMID:27252732

  7. Characterization of an Autophagy-Related Gene MdATG8i from Apple

    PubMed Central

    Wang, Ping; Sun, Xun; Jia, Xin; Wang, Na; Gong, Xiaoqing; Ma, Fengwang

    2016-01-01

    Nutrient deficiencies restrict apple (Malus sp.) tree growth and productivity in Northwest China. The process of autophagy, a conserved degradation pathway in eukaryotic cells, has important roles in nutrient-recycling and helps improve plant performance during periods of nutrient-starvation. Little is known about the functioning of autophagy-related genes (ATGs) in apple. In this study, one of the ATG8 gene family members MdATG8i was isolated from Malus domestica. MdATG8i has conserved putative tubulin binding sites and ATG7 interaction domains. A 1865-bp promoter region cloned from apple genome DNA was predicated to have cis-regulatory elements responsive to light, environmental stresses, and hormones. MdATG8i transcriptions were induced in response to leaf senescence, nitrogen depletion, and oxidative stress. At cellular level, MdATG8i protein was expressed in the nucleus and cytoplasm of onion epidermal cells. Yeast two-hybrid tests showed that MdATG8i could interact with MdATG7a and MdATG7b. In Arabidopsis, its heterologous expression was associated with enhanced vegetative growth, leaf senescence, and tolerance to nitrogen- and carbon-starvation. MdATG8i-overexpressing “Orin” apple callus lines also displayed improved tolerance to nutrient-limited conditions. Our results demonstrate that MdATG8i protein could function in autophagy in a conserved way, as a positive regulator in the response to nutrient-starvation. PMID:27252732

  8. 77 FR 68149 - Karen Paul Holley, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-15

    ... essential condition for obtaining and maintaining a DEA registration. Serenity Caf , 77 FR 35027, 35028 (2012); David W. Wang, 72 FR 54297, 54298 (2007); Sheran Arden Yeates, 71 FR 39130, 39131 (2006); Dominick A. Ricci, M.D., 58 FR 51104 (1993); Bobby Watts, M.D., 53 FR 11919 (1988). Because...

  9. 78 FR 12091 - Brian Earl Cressman, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-21

    ... proceeding is not required. See Jesus R. Juarez, M.D., 62 FR 14945 (1997); Dominick A. Ricci, M.D., 58 FR..., 873 F.2d 1089, 1091 (8th Cir. 1989); Thomas E. Johnston, 45 FR 72311 (1980). The Controlled Substances... DEA registration. Serenity Caf , 77 FR 35027, 35028 (2012); David W. Wang, 72 FR 54297, 54298...

  10. 78 FR 59060 - Gabriel Sanchez, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-25

    ... sufficiently reliable to be accepted and relied upon in this .'' See Cynthia M. Cadet, M.D., 76 FR 19450, 19458... factors are . . . considered in the disjunctive.'' Robert A. Leslie, M.D., 68 FR 15227, 15230 (2003). It... Krishna-Iyer, 74 FR 459, 462 (2009). Accordingly, as the Tenth Circuit has recognized, findings under...

  11. 33 CFR 110.70 - Chesapeake and Delaware Canal, easterly of Courthouse Point, Md.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Chesapeake and Delaware Canal, easterly of Courthouse Point, Md. 110.70 Section 110.70 Navigation and Navigable Waters COAST GUARD... and Delaware Canal, easterly of Courthouse Point, Md. The waters southerly of a line joining...

  12. 76 FR 75448 - Establishment of Class D and E Airspace; Frederick, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-02

    ... Class D and E airspace at Frederick, MD, to accommodate new Area Navigation (RNAV) Global Positioning System (GPS) Standard Instrument Approach Procedures (SIAPs) at Frederick Municipal Airport. This action... and E airspace for the new Frederick Municipal Airport, Frederick, MD (76 FR 50156) Docket No....

  13. 33 CFR 165.509 - Security Zone; Severn River and College Creek, Annapolis, MD.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... College Creek, Annapolis, MD. 165.509 Section 165.509 Navigation and Navigable Waters COAST GUARD... § 165.509 Security Zone; Severn River and College Creek, Annapolis, MD. (a) Definitions. For purposes of... the Naval Academy waterfront. This security zone includes the waters of College Creek eastward of...

  14. 75 FR 8749 - Dwayne LaFrantz Wilson, M.D.; Revocation of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... Enforcement Administration Dwayne LaFrantz Wilson, M.D.; Revocation of Registration On October 22, 2008, the... Order to Show Cause to Dwayne LaFrantz Wilson, M.D. (Respondent), of Providence, Rhode Island. The Show... suspended, and that he therefore lacks authority to handle controlled substances under the laws of...

  15. 77 FR 73677 - Stephanie A. Tarapchak, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ... . . . dispensing of controlled substances.'' See, e.g., Richard H. Ng, 77 FR 29694 (2012); Segun M. Rasaki, 77 FR 29692 (2012); David W. Wang, 72 FR 54297 (2007). Rather, DEA's rule derives primarily from two other... (quoting Calvin Ramsey, M.D., 76 FR 20034, 20036 (2011)). See also Kamal Tiwari, M.D., 76 FR 71604,...

  16. 77 FR 22523 - Safety Zone; 2012 Ocean City Air Show; Atlantic Ocean, Ocean City, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-16

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; 2012 Ocean City Air Show; Atlantic Ocean... proposes establishing a safety zone on the navigable waters of the Atlantic Ocean in Ocean City, MD. This..., 2012, the Town of Ocean City will host an air show event over the Atlantic Ocean in Ocean City, MD....

  17. 77 FR 57116 - Cleveland J. Enmon, Jr., M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... Enforcement Administration Cleveland J. Enmon, Jr., M.D.; Decision and Order On April 26, 2012, Administrative... 1177, 1182 (11th Cir. 2008); J.A.M. Builders v. Herman, 233 F.3d 1350, 1354 (11th Cir. 2000); Hoska v... that DEA Certificate of Registration No. BE9655284, issued to Cleveland J. Enmon, Jr., M.D., be, and...

  18. 75 FR 76688 - Drawbridge Operation Regulation; Isle of Wight (Sinepuxent) Bay, Ocean City, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-09

    ... (Sinepuxent) Bay, Ocean City, MD AGENCY: Coast Guard, DHS. ACTION: Notice of proposed rulemaking. SUMMARY: The... of Wight (Sinepuxent) Bay, mile 0.5, at Ocean City, MD. This proposed rule will require any mariner... opening. The Coast Guard also proposes to change the waterway location from Isle of Wight Bay to Isle...

  19. 33 CFR 110.71 - Jacobs Nose Cove, Elk River, Md.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Jacobs Nose Cove, Elk River, Md... ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.71 Jacobs Nose Cove, Elk River, Md. The water area of Jacobs Nose Cove, on the west side of the mouth of Elk River, Maryland, comprising the...

  20. 33 CFR 110.71 - Jacobs Nose Cove, Elk River, Md.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Jacobs Nose Cove, Elk River, Md... ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.71 Jacobs Nose Cove, Elk River, Md. The water area of Jacobs Nose Cove, on the west side of the mouth of Elk River, Maryland, comprising the...

  1. 33 CFR 110.71 - Jacobs Nose Cove, Elk River, Md.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Jacobs Nose Cove, Elk River, Md... ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.71 Jacobs Nose Cove, Elk River, Md. The water area of Jacobs Nose Cove, on the west side of the mouth of Elk River, Maryland, comprising the...

  2. 33 CFR 110.71 - Jacobs Nose Cove, Elk River, Md.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Jacobs Nose Cove, Elk River, Md... ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.71 Jacobs Nose Cove, Elk River, Md. The water area of Jacobs Nose Cove, on the west side of the mouth of Elk River, Maryland, comprising the...

  3. 33 CFR 110.71 - Jacobs Nose Cove, Elk River, Md.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Jacobs Nose Cove, Elk River, Md... ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.71 Jacobs Nose Cove, Elk River, Md. The water area of Jacobs Nose Cove, on the west side of the mouth of Elk River, Maryland, comprising the...

  4. 77 FR 35021 - Kwan Bo Jin, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-12

    ... revoking Respondent's DEA registration. See Johnnie Melvin Turner, M.D., 67 FR 71,203, 71,204 (DEA 2002... 62,095 (DEA 2004) (respondent waived hearing); \\23\\ Johnnie-Melvin Turner, M.D., 67 FR 71,203 (DEA..., at 14-16; \\1\\ which is contrary to agency precedent.\\2\\ See Linda Sue Cheek, 76 FR 66972, 66973...

  5. 33 CFR 165.512 - Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., Northwest and Inner Harbors, Baltimore, MD. 165.512 Section 165.512 Navigation and Navigable Waters COAST... Guard District § 165.512 Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD. (a... CONSTELLATION, while operating in the Inner Harbor, the Northwest Harbor and the Patapsco River. (c)...

  6. 33 CFR 165.512 - Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., Northwest and Inner Harbors, Baltimore, MD. 165.512 Section 165.512 Navigation and Navigable Waters COAST... Guard District § 165.512 Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD. (a... CONSTELLATION, while operating in the Inner Harbor, the Northwest Harbor and the Patapsco River. (c)...

  7. 33 CFR 165.512 - Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Northwest and Inner Harbors, Baltimore, MD. 165.512 Section 165.512 Navigation and Navigable Waters COAST... Guard District § 165.512 Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD. (a... CONSTELLATION, while operating in the Inner Harbor, the Northwest Harbor and the Patapsco River. (c)...

  8. 33 CFR 165.512 - Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., Northwest and Inner Harbors, Baltimore, MD. 165.512 Section 165.512 Navigation and Navigable Waters COAST... Guard District § 165.512 Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD. (a... CONSTELLATION, while operating in the Inner Harbor, the Northwest Harbor and the Patapsco River. (c)...

  9. 33 CFR 165.512 - Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., Northwest and Inner Harbors, Baltimore, MD. 165.512 Section 165.512 Navigation and Navigable Waters COAST... Guard District § 165.512 Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD. (a... CONSTELLATION, while operating in the Inner Harbor, the Northwest Harbor and the Patapsco River. (c)...

  10. 75 FR 21167 - Special Local Regulations for Marine Events; Chester River, Chestertown, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-23

    ... Regulations for Marine Events; Chester River, Chestertown, MD'' in the Federal Register (75 FR 043). We... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA08 Special Local Regulations for Marine Events; Chester... Party Festival'', a marine event to be held on the waters of the Chester River, Chestertown, MD on...

  11. 76 FR 29640 - Special Local Regulations for Marine Events; Chester River, Chestertown, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-23

    ... Regulations for Marine Events; Chester River, Chestertown, MD'' in the Federal Register (76 FR 54). We... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations for Marine Events; Chester... Party Festival,'' a marine event to be held on the waters of the Chester River, Chestertown, MD on...

  12. 75 FR 57388 - Special Local Regulations for Marine Events; Patuxent River, Solomons, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-21

    ... Marine Events; Patuxent River, Solomons, MD'' in the Federal Register (75 FR 32866). Additionally, on... Local Regulations for Marine Events; Patuxent River, Solomons, MD'' in the Federal Register (75 FR 41789... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations for Marine Events;...

  13. 76 FR 23185 - Drawbridge Operation Regulation; Isle of Wight (Sinepuxent) Bay, Ocean City, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-26

    ... (Sinepuxent) Bay, Ocean City, MD AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast Guard is... (Sinepuxent) Bay, Ocean City, MD in the Federal Register (75 FR 236). We received no comments on the proposed... fields, and governmental jurisdictions with populations of less than 50,000. The Coast Guard...

  14. 77 FR 23599 - Special Local Regulations for Marine Events; Potomac River, Charles County, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-20

    .... Ronald Houck, U.S. Coast Guard Sector Baltimore, MD; telephone 410-576-2674, email Ronald.L.Houck@uscg...; Potomac River, Charles County, MD'' in the Federal Register (77 FR 27). We received one comment on the... the commenter concerning the proposed regulations. The commenter, Mr. David R. Horning of...

  15. 76 FR 16296 - Drawbridge Operation Regulation; Pocomoke River, Snow Hill, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-23

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulation; Pocomoke River, Snow Hill, MD AGENCY... the S12 Bridge across Pocomoke River, mile 29.9, at Snow Hill, MD. The deviation restricts the... least five hours advance notice is given. The S12 Bridge across Pocomoke River, mile 29.9 at Snow...

  16. 77 FR 29692 - Segun M. Rasaki, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ...., 67 FR 35,582 (DEA 2002); Michael G. Dolin, M.D., 65 Fed. Reg. 5661 (DEA 2000); see also Philip E...'s action at which he may ultimately prevail.'' Kamal Tiwari, M.D., 76 FR 71604, 71606 (2011); see also Bourne Pharmacy, Inc., 72 FR 18273, 18274 (2007); Anne Lazar Thorn, 62 FR 12847...

  17. MD Simulations of P-Type ATPases in a Lipid Bilayer System.

    PubMed

    Autzen, Henriette Elisabeth; Musgaard, Maria

    2016-01-01

    Molecular dynamics (MD) simulation is a computational method which provides insight on protein dynamics with high resolution in both space and time, in contrast to many experimental techniques. MD simulations can be used as a stand-alone method to study P-type ATPases as well as a complementary method aiding experimental studies. In particular, MD simulations have proved valuable in generating and confirming hypotheses relating to the structure and function of P-type ATPases. In the following, we describe a detailed practical procedure on how to set up and run a MD simulation of a P-type ATPase embedded in a lipid bilayer using software free of use for academics. We emphasize general considerations and problems typically encountered when setting up simulations. While full coverage of all possible procedures is beyond the scope of this chapter, we have chosen to illustrate the MD procedure with the Nanoscale Molecular Dynamics (NAMD) and the Visual Molecular Dynamics (VMD) software suites.

  18. Center of excellence for mobile sensor data-to-knowledge (MD2K).

    PubMed

    Kumar, Santosh; Abowd, Gregory D; Abraham, William T; al'Absi, Mustafa; Beck, J Gayle; Chau, Duen Horng; Condie, Tyson; Conroy, David E; Ertin, Emre; Estrin, Deborah; Ganesan, Deepak; Lam, Cho; Marlin, Benjamin; Marsh, Clay B; Murphy, Susan A; Nahum-Shani, Inbal; Patrick, Kevin; Rehg, James M; Sharmin, Moushumi; Shetty, Vivek; Sim, Ida; Spring, Bonnie; Srivastava, Mani; Wetter, David W

    2015-11-01

    Mobile sensor data-to-knowledge (MD2K) was chosen as one of 11 Big Data Centers of Excellence by the National Institutes of Health, as part of its Big Data-to-Knowledge initiative. MD2K is developing innovative tools to streamline the collection, integration, management, visualization, analysis, and interpretation of health data generated by mobile and wearable sensors. The goal of the big data solutions being developed by MD2K is to reliably quantify physical, biological, behavioral, social, and environmental factors that contribute to health and disease risk. The research conducted by MD2K is targeted at improving health through early detection of adverse health events and by facilitating prevention. MD2K will make its tools, software, and training materials widely available and will also organize workshops and seminars to encourage their use by researchers and clinicians. PMID:26555017

  19. Center of excellence for mobile sensor data-to-knowledge (MD2K)

    PubMed Central

    Kumar, Santosh; Abowd, Gregory D; Abraham, William T; al’Absi, Mustafa; Gayle Beck, J; Chau, Duen Horng; Condie, Tyson; Conroy, David E; Ertin, Emre; Estrin, Deborah; Ganesan, Deepak; Lam, Cho; Marlin, Benjamin; Marsh, Clay B; Murphy, Susan A; Nahum-Shani, Inbal; Patrick, Kevin; Rehg, James M; Sharmin, Moushumi; Shetty, Vivek; Sim, Ida; Spring, Bonnie; Srivastava, Mani; Wetter, David W

    2015-01-01

    Mobile sensor data-to-knowledge (MD2K) was chosen as one of 11 Big Data Centers of Excellence by the National Institutes of Health, as part of its Big Data-to-Knowledge initiative. MD2K is developing innovative tools to streamline the collection, integration, management, visualization, analysis, and interpretation of health data generated by mobile and wearable sensors. The goal of the big data solutions being developed by MD2K is to reliably quantify physical, biological, behavioral, social, and environmental factors that contribute to health and disease risk. The research conducted by MD2K is targeted at improving health through early detection of adverse health events and by facilitating prevention. MD2K will make its tools, software, and training materials widely available and will also organize workshops and seminars to encourage their use by researchers and clinicians. PMID:26555017

  20. H5MD: A structured, efficient, and portable file format for molecular data

    NASA Astrophysics Data System (ADS)

    de Buyl, Pierre; Colberg, Peter H.; Höfling, Felix

    2014-06-01

    We propose a new file format named "H5MD" for storing molecular simulation data, such as trajectories of particle positions and velocities, along with thermodynamic observables that are monitored during the course of the simulation. H5MD files are HDF5 (Hierarchical Data Format) files with a specific hierarchy and naming scheme. Thus, H5MD inherits many benefits of HDF5, e.g., structured layout of multi-dimensional datasets, data compression, fast and parallel I/O, and portability across many programming languages and hardware platforms. H5MD files are self-contained, and foster the reproducibility of scientific data and the interchange of data between researchers using different simulation programs and analysis software. In addition, the H5MD specification can serve for other kinds of data (e.g. experimental data) and is extensible to supplemental data, or may be part of an enclosing file structure.

  1. MD studies on conformational behavior of a DNA photolyase enzyme

    NASA Astrophysics Data System (ADS)

    Dushanov, E.; Kholmurodov, Kh.; Yasuoka, K.; Krasavin, E.

    2013-11-01

    In this work, molecular dynamics (MD) simulations were performed on a DNA photolyase protein with two cofactors, FAD (flavin adenine dinucleotide) and MTHF (methenyltetrahydrofolate), inside the enzyme pocket. A DNA photolyase is a highly efficient light-driven enzyme that repairs the UV-induced cyclobutane-pyrimidine dimer in damaged DNA. We were aimed to compare the conformational changes of the FAD cofactor and other constituent fragments of the molecular system under consideration. The conformational behavior of the FAD molecule is very important for understanding the functional and structural properties of the DNA repair protein photolyase. The photoactive FAD is an essential cofactor both for specificial binding to damaged DNA and for catalysis. The second chromophore (MTHF or 8-HDF) is not necessary for catalysis and has no effect on specific enzyme—substrate binding. The obtained results were discussed to gain insight into the light-driven mechanism of DNA repair by a DNA photolyase enzyme—based on the enzyme structure, the FAD mobility, and conformation shape.

  2. Breast reconstruction at the MD Anderson Cancer Center

    PubMed Central

    2016-01-01

    The introduction of the transverse rectus abdominis myocutaneous flap in the 1970s marks the beginning of modern breast reconstruction although implants were available even earlier mainly for breast augmentation. Mastectomy techniques have evolved from the early Halsted radical mastectomy to the modern skin sparing mastectomy. The latter made possible using implants for breast reconstruction. Although prosthetic reconstruction provides a simpler procedure with quick recovery, autologous reconstruction offers more natural and long-lasting results especially in the setting of radiotherapy. Both forms have been extensively used at the MD Anderson Cancer Center (MDACC) while microsurgical breast reconstruction has been the hallmark of the MDACC experience. One of the most challenging areas of breast reconstruction is how to achieve good results without compromising adjuvant therapy when post-mastectomy radiotherapy is required. Managing upper extremity lymphedema following breast cancer treatment is another difficult issue which has gained great attention in recent years. This article highlights the important work in various aspects of breast reconstruction that has been done at the MDACC. PMID:27563563

  3. Using MD Simulations To Calculate How Solvents Modulate Solubility.

    PubMed

    Liu, Shuai; Cao, Shannon; Hoang, Kevin; Young, Kayla L; Paluch, Andrew S; Mobley, David L

    2016-04-12

    Here, our interest is in predicting solubility in general, and we focus particularly on predicting how the solubility of particular solutes is modulated by the solvent environment. Solubility in general is extremely important, both for theoretical reasons - it provides an important probe of the balance between solute-solute and solute-solvent interactions - and for more practical reasons, such as how to control the solubility of a given solute via modulation of its environment, as in process chemistry and separations. Here, we study how the change of solvent affects the solubility of a given compound. That is, we calculate relative solubilities. We use MD simulations to calculate relative solubility and compare our calculated values with experiment as well as with results from several other methods, SMD and UNIFAC, the latter of which is commonly used in chemical engineering design. We find that straightforward solubility calculations based on molecular simulations using a general small-molecule force field outperform SMD and UNIFAC both in terms of accuracy and coverage of the relevant chemical space. PMID:26878198

  4. Breast reconstruction at the MD Anderson Cancer Center.

    PubMed

    Yu, Peirong

    2016-08-01

    The introduction of the transverse rectus abdominis myocutaneous flap in the 1970s marks the beginning of modern breast reconstruction although implants were available even earlier mainly for breast augmentation. Mastectomy techniques have evolved from the early Halsted radical mastectomy to the modern skin sparing mastectomy. The latter made possible using implants for breast reconstruction. Although prosthetic reconstruction provides a simpler procedure with quick recovery, autologous reconstruction offers more natural and long-lasting results especially in the setting of radiotherapy. Both forms have been extensively used at the MD Anderson Cancer Center (MDACC) while microsurgical breast reconstruction has been the hallmark of the MDACC experience. One of the most challenging areas of breast reconstruction is how to achieve good results without compromising adjuvant therapy when post-mastectomy radiotherapy is required. Managing upper extremity lymphedema following breast cancer treatment is another difficult issue which has gained great attention in recent years. This article highlights the important work in various aspects of breast reconstruction that has been done at the MDACC. PMID:27563563

  5. The value circle. A profile of J. Richard Gaintner, MD.

    PubMed

    Howard, R

    1999-01-01

    This article describes how the arrival of CEO J. Richard Gaintner, MD, at Shands HealthCare signaled a time for refocusing the organization's direction and helping physicians to cope with the changes buffeting the industry. He saw angst and disenfranchisement, sentiments that characterized not only Shands and the University of Florida Health Science Center, but also the entire establishment of American scientific medicine. Gaintner believes--and continually preaches--that practicing medicine in a cost-effective manner will improve, not harm, the quality of care. His willingness to face reality objectively is perhaps his greatest asset in helping physicians deal with managed care. He conveys heartfelt empathy with the day-to-day conflicts they face. But he does not allow himself the temporary luxury of cynicism, and he refuses to accept negativity and pessimism in others. Rather, he asks that physicians and managers understand the system and develop the capacity to work within it and take responsibility for improving it. Beyond exhorting physicians to be accountable for the success of the enterprise, Gaintner creates mechanisms for meaningful physician participation in enterprise management. PMID:10351728

  6. John Call Dalton, Jr., MD: America's first neurophysiologist.

    PubMed

    Fine, E J; Manteghi, T; Sobel, S H; Lohr, L A

    2000-09-26

    Before the discoveries of John Call Dalton, Jr., MD (1824-1889), innervation of laryngeal muscles, long-term effects of cerebellar lesions, and consequences of raised intracranial pressure were poorly understood. Dalton discovered that the posterior cricoarytenoid muscles adducted the vocal cords during inspiration. He confirmed Flourens' observations that acute ablation of the cerebellum of pigeons caused loss of coordination. Dalton observed that properly cared for pigeons gradually recovered "coordinating power." Dalton observed that prolonged raised intracranial pressure caused tachycardia and then fatal bradycardia in dogs. Before Dalton published his photographic atlas of the human brain, neuroanatomy atlases were sketched by Europeans and imported into the United States. Dalton's atlas of the human brain contained precise photographs of vertical and horizontal sections that equal modern works. Before Dalton introduced live demonstrations of animals, physiology was taught by recitation of texts only. Dalton was the first American-born professor to teach physiology employing demonstrations of live animals operated on under ether anesthesia. He wrote an essay advocating experimentation on animals as the proper method of acquiring knowledge of function and that humane animal experimentation would ultimately improve the health of man and animals. His eloquent advocacy for humane experimental physiology quelled attacks by contemporaneous antivivisectionists. Dalton was America's first experimental neurophysiologist.

  7. Md1 and Rp105 regulate innate immunity and viral resistance in zebrafish.

    PubMed

    Candel, Sergio; Sepulcre, María P; Espín-Palazón, Raquel; Tyrkalska, Sylwia D; de Oliveira, Sofía; Meseguer, José; Mulero, Victoriano

    2015-06-01

    TLR4 was the first TLR family member identified in mammals and is responsible for the activation of the immune response by bacterial LPS. Later, MD1 and RP105 were shown to form complexes that directly interact with the MD2-TLR4 complex, acting as physiological negative regulators of LPS signaling. Despite the general conservation of various TLR families from fish to mammals, several differences can be appreciated, such as the high tolerance of fish to LPS, the absence of the crucial accessory molecules Md2 and Cd14 for Tlr4 signaling in fish, the absence of Tlr4 in some fish species, and the confirmation that LPS does not signal through Tlr4 in zebrafish. The present study has identified the Rp105 and Md1 homologs in zebrafish, confirming (i) Rp105 and Tlr4 evolved from a common ancestor before the divergence between fish and tetrapods and (ii) the presence of Md1 in teleost fish and the lack of Md2, suggesting that the divergence of these accessory molecules occurred in the tetrapod lineage. Biochemical and functional studies indicate that Md1 binds both Rp105 and Tlr4 in zebrafish. Genetic inhibition of zebrafish Md1 and Rp105 reveals that Md1 or Rp105 deficiency impairs the expression of genes encoding pro-inflammatory and antiviral molecules, leading to increased susceptibility to viral infection. These results shed light on the evolutionary history of Md1 and Rp105 and uncover a previously unappreciated function of these molecules in the regulation of innate immunity.

  8. Studies of the TLR4-associated protein MD-2 using yeast-display and mutational analyses.

    PubMed

    Mattis, Daiva M; Chervin, Adam S; Ranoa, Diana R; Kelley, Stacy L; Tapping, Richard I; Kranz, David M

    2015-12-01

    Bacterial lipopolysaccharide (LPS) activates the innate immune system by forming a complex with myeloid differentiation factor 2 (MD-2) and Toll-like receptor 4 (TLR4), which is present on antigen presenting cells. MD-2 plays an essential role in this activation of the innate immune system as a member of the ternary complex, TLR4:MD-2:LPS. With the goal of further understanding the molecular details of the interaction of MD-2 with LPS and TLR4, and possibly toward engineering dominant negative regulators of the MD-2 protein, here we subjected MD-2 to a mutational analysis using yeast display. The approach included generation of site-directed alanine mutants, and ligand-driven selections of MD-2 mutant libraries. Our findings showed that: (1) proline mutations in the F119-K132 loop that binds LPS were strongly selected for enhanced yeast surface stability, (2) there was a preference for positive-charged side chains (R/K) at residue 120 for LPS binding, and negative-charged side chains (D/E) for TLR4 binding, (3) aromatic residues were strongly preferred at F119 and F121 for LPS binding, and (4) an MD-2 mutant (T84N/D101A/S118A/S120D/K122P) exhibited increased binding to TLR4 but decreased binding to LPS. These studies revealed the impact of specific residues and regions of MD-2 on the binding of LPS and TLR4, and they provide a framework for further directed evolution of the MD-2 protein. PMID:26320630

  9. 33 CFR 162.40 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... section in 33 CFR part 207. ... River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal). 162.40 Section 162.40 Navigation... Bay, Del. and Md. (Chesapeake and Delaware Canal). (a) Applicability. The regulations in this...

  10. MdERFs, two ethylene-response factors involved in apple fruit ripening.

    PubMed

    Wang, Aide; Tan, Dongmei; Takahashi, Ayako; Li, Tian Zhong; Harada, Takeo

    2007-01-01

    Two MdERFs (ethylene-response factors) were isolated from ripening apple (Malusxdomestica Borkh. cv. Golden Delicious) fruit. The features of their conserved motifs indicated that MdERF1 and MdERF2 belong to group VII and group IX categories in Arabidopsis, respectively. MdERF1 was expressed predominantly in ripening fruit, although a small degree of expression was also observed in non-fruit tissues, whereas MdERF2 was expressed exclusively in ripening fruit. The increased expression in ripening fruit was repressed by treatment with 1-methylcyclopropene (1-MCP: a potent antagonist of ethylene receptors), indicating that transcription is regulated positively by the ethylene signalling system. Indeed, it was a tendency for cultivars with low ethylene production to show lower MdERFs expression than those with high ethylene production. On the basis of concomitant analyses of the expression of some genes related to ripening, the functions of MdERFs and the role of ethylene in the ripening process are discussed.

  11. A hybrid MD-DSMC coupling method to investigate flow characteristics of micro-devices

    NASA Astrophysics Data System (ADS)

    Watvisave, D. S.; Puranik, B. P.; Bhandarkar, U. V.

    2015-12-01

    A new methodology is proposed to couple Molecular Dynamics (MD) and Direct Simulation Monte Carlo (DSMC) methods to simulate high Knudsen number (Kn) flows. For this purpose a two-dimensional hybrid MD-DSMC code is developed. In this method gas-surface interactions are modeled using MD, and gas-gas interactions are modeled using DSMC method. Two-way coupling between MD and DSMC is implemented by employing buffer zones for both MD and DSMC regions. Bootstrap sampling and energy minimization algorithms are employed for dynamic coupling of these two methods since MD utilizes real number of molecules during simulation whereas DSMC utilizes a lesser number of simulated molecules. The hybrid methodology combines the advantages of both methods; it has the capability of modeling the gas-surface interaction accurately considering the effect of the presence of neighboring real number of gas molecules, while in the bulk it utilizes DSMC with only the simulated number of molecules thus increasing the computational efficiency significantly compared to pure MD codes. As a result comparatively large domain sizes can be simulated with realistic behavior at the walls. The utility of the hybrid method is demonstrated by simulating high Kn flows through a micro-channel, micro-nozzle and micro-scale shock tube. The effect of partial accommodation of gas molecules with the wall is seen to be captured dynamically with this approach.

  12. Electroporation of archaeal lipid membranes using MD simulations.

    PubMed

    Polak, Andraž; Tarek, Mounir; Tomšič, Matija; Valant, Janez; Ulrih, Nataša Poklar; Jamnik, Andrej; Kramar, Peter; Miklavčič, Damijan

    2014-12-01

    Molecular dynamics (MD) simulations were used to investigate the electroporation of archaeal lipid bilayers when subjected to high transmembrane voltages induced by a charge imbalance, mimicking therefore millisecond electric pulse experiments. The structural characteristics of the bilayer, a 9:91 mol% 2,3-di-O-sesterterpanyl-sn-glicerol-1-phospho-myo-inositol (AI) and 2,3-di-O-sesterterpanyl-sn-glicerol-1-phospho-1'(2'-O-α-D-glucosyl)-myo-inositol (AGI) were compared to small angle X-ray scattering data. A rather good agreement of the electron density profiles at temperatures of 298 and 343 K was found assessing therefore the validity of the protocols and force fields used in simulations. Compared to dipalmitoyl-phosphatidylcholine (DPPC), the electroporation threshold for the bilayer was found to increase from ~2 V to 4.3 V at 323 K, and to 5.2 V at 298 K. Comparing the electroporation thresholds of the archaeal lipids to those of simple diphytanoyl-phosphatidylcholine (DPhPC) bilayers (2.5 V at 323 K) allowed one to trace back the stability of the membranes to the structure of their lipid head groups. Addition of DPPC in amounts of 50 mol% to the archaeal lipid bilayers decreases their stability and lowers the electroporation thresholds to 3.8 V and 4.1 V at respectively 323 and 298 K. The present study therefore shows how membrane compositions can be selected to cover a wide range of responses to electric stimuli. This provides new routes for the design of liposomes that can be efficiently used as drug delivery carriers, as the selection of their composition allows one to tune in their electroporation threshold for subsequent release of their load. PMID:24461702

  13. Electroporation of archaeal lipid membranes using MD simulations.

    PubMed

    Polak, Andraž; Tarek, Mounir; Tomšič, Matija; Valant, Janez; Ulrih, Nataša Poklar; Jamnik, Andrej; Kramar, Peter; Miklavčič, Damijan

    2014-12-01

    Molecular dynamics (MD) simulations were used to investigate the electroporation of archaeal lipid bilayers when subjected to high transmembrane voltages induced by a charge imbalance, mimicking therefore millisecond electric pulse experiments. The structural characteristics of the bilayer, a 9:91 mol% 2,3-di-O-sesterterpanyl-sn-glicerol-1-phospho-myo-inositol (AI) and 2,3-di-O-sesterterpanyl-sn-glicerol-1-phospho-1'(2'-O-α-D-glucosyl)-myo-inositol (AGI) were compared to small angle X-ray scattering data. A rather good agreement of the electron density profiles at temperatures of 298 and 343 K was found assessing therefore the validity of the protocols and force fields used in simulations. Compared to dipalmitoyl-phosphatidylcholine (DPPC), the electroporation threshold for the bilayer was found to increase from ~2 V to 4.3 V at 323 K, and to 5.2 V at 298 K. Comparing the electroporation thresholds of the archaeal lipids to those of simple diphytanoyl-phosphatidylcholine (DPhPC) bilayers (2.5 V at 323 K) allowed one to trace back the stability of the membranes to the structure of their lipid head groups. Addition of DPPC in amounts of 50 mol% to the archaeal lipid bilayers decreases their stability and lowers the electroporation thresholds to 3.8 V and 4.1 V at respectively 323 and 298 K. The present study therefore shows how membrane compositions can be selected to cover a wide range of responses to electric stimuli. This provides new routes for the design of liposomes that can be efficiently used as drug delivery carriers, as the selection of their composition allows one to tune in their electroporation threshold for subsequent release of their load.

  14. Paleomagnetic Study of Core MD012380 from Banda Sea

    NASA Astrophysics Data System (ADS)

    Lee, T.; Huang, Y.; Chen, C.; Yang, T.; Hsu, S.; Wei, K.

    2003-12-01

    This study presents the paleomagnetic results of core MD012380 taken from Banda Sea during IMAGES VII cruise in 2001. The locality of the coring site is 126o 54.25­ÝE and 5o45.64­ÝS, and the water depth is about 3232m. The total recovered length of this core is 39.9 m. The simulated paleo-intensity pattern is well correlated to that of the SINT 800 (Guyodo and Valet, 1999). Several magnetic events and excursions, such as Blake, Jarmaica, Lavantine, Big Lost Emperor, Delta and B/M Boundary could be identified. These events appeared at the depths of about 13.84 m, 18.05 m, 22.75 m, 31.81 m, 35.89 m, 38.70 m, respectively. Paleoinclination data also indicate that the boundary of Brunhes and Matuyama epochs appeared at the depth of 38.7 m. So, the paleomagnetic result is used to construct the age model of this core. Then, apply it to the studied oxygen isotope pattern and compare to that proposed by Bassinot et al. (1994), they are well correlated. In addition, the LAD of P. lacunosa is found at the depth of 28.4 m, which also supports the proposed age model. Result of magnetic susceptibility (c) shows several high peaks, especially at the bottom portion, which might relate to the volcanic activity in the surrounding area. Generally, high values occurred at the interglacial periods and low values appeared at the interglacial periods. However, magnetic susceptibility has a special increasing trend from stage 8 to the early stage 5. It may be worthy of further study.

  15. 33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and... Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and navigation. (a... River to Chesapeake Bay, Del. and Md., between Reedy Point, Delaware River, and Old Town Point...

  16. 33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and... Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and navigation. (a... River to Chesapeake Bay, Del. and Md., between Reedy Point, Delaware River, and Old Town Point...

  17. 33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and... Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and navigation. (a... River to Chesapeake Bay, Del. and Md., between Reedy Point, Delaware River, and Old Town Point...

  18. 33 CFR 110.72a - Chester River, southeast of Chestertown, Md.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Chestertown, Md. The waters of the Chester River enclosed by a line beginning at a point on the Rolph Marina... Point Marina pier; thence southwesterly along the Rolph Point Marina pier to the point of beginning....

  19. 33 CFR 110.72a - Chester River, southeast of Chestertown, Md.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Chestertown, Md. The waters of the Chester River enclosed by a line beginning at a point on the Rolph Marina... Point Marina pier; thence southwesterly along the Rolph Point Marina pier to the point of beginning....

  20. 75 FR 31835 - Environmental Impact Statement: Calvert and St. Mary's Counties, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ... Counties, Maryland (Federal Register Vol. 72, No. 203; FR Doc. 07-5190) is being withdrawn and an..., pedestrian and bicycle safety along MD 4, while supporting existing and planned development in the...

  1. 33 CFR 110.72a - Chester River, southeast of Chestertown, Md.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Chestertown, Md. The waters of the Chester River enclosed by a line beginning at a point on the Rolph Marina... Point Marina pier; thence southwesterly along the Rolph Point Marina pier to the point of beginning....

  2. 33 CFR 110.72a - Chester River, southeast of Chestertown, Md.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Chestertown, Md. The waters of the Chester River enclosed by a line beginning at a point on the Rolph Marina... Point Marina pier; thence southwesterly along the Rolph Point Marina pier to the point of beginning....

  3. 33 CFR 110.72a - Chester River, southeast of Chestertown, Md.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Chestertown, Md. The waters of the Chester River enclosed by a line beginning at a point on the Rolph Marina... Point Marina pier; thence southwesterly along the Rolph Point Marina pier to the point of beginning....

  4. Osler's Pupil, Henry W. Ochsner, MD (1877–1902): His Life, Lineage, and Death

    PubMed Central

    Mueller, Paul S.

    2007-01-01

    In multiple editions of his Principles and Practice of Medicine, a 1904 speech, and his essay “A Student Life,” Sir William Osler mentions and laments the death due to typhoid of his pupil, Henry W. Ochsner (1877–1902). Harvey Cushing, MD, in his biography of Osler, describes how deeply Osler was moved by “poor” Ochsner's death. Yet little is known about Ochsner. This article describes the life story, lineage, and death of Henry W. Ochsner, MD, a son of Swiss pioneers who settled in Waumandee, Wisconsin. He was a member of a family that includes medical luminaries (e.g., Albert J. Ochsner, MD, the famous Chicago surgeon, and Alton Ochsner, MD, the founder of the Ochsner Clinic); a brilliant student and physician; a humble and beloved fellow citizen; and a favorite pupil of Osler. PMID:21603518

  5. 78 FR 38000 - Special Local Regulations; Marine Events, Spa Creek and Annapolis Harbor; Annapolis, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-25

    ...; Annapolis, MD'' in the Federal Register (78 FR 20066). The rulemaking concerned the Coast Guard's proposal... regulations. The rulemaking was initiated to establish special local regulations during the swim segment...

  6. MdCOP1 Ubiquitin E3 Ligases Interact with MdMYB1 to Regulate Light-Induced Anthocyanin Biosynthesis and Red Fruit Coloration in Apple1[W][OA

    PubMed Central

    Li, Yuan-Yuan; Mao, Ke; Zhao, Cheng; Zhao, Xian-Yan; Zhang, Hua-Lei; Shu, Huai-Rui; Hao, Yu-Jin

    2012-01-01

    MdMYB1 is a crucial regulator of light-induced anthocyanin biosynthesis and fruit coloration in apple (Malus domestica). In this study, it was found that MdMYB1 protein accumulated in the light but degraded via a ubiquitin-dependent pathway in the dark. Subsequently, the MdCOP1-1 and MdCOP1-2 genes were isolated from apple fruit peel and were functionally characterized in the Arabidopsis (Arabidopsis thaliana) cop1-4 mutant. Yeast (Saccharomyces cerevisiae) two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays showed that MdMYB1 interacts with the MdCOP1 proteins. Furthermore, in vitro and in vivo experiments indicated that MdCOP1s are necessary for the ubiquitination and degradation of MdMYB1 protein in the dark and are therefore involved in the light-controlled stability of the MdMYB1 protein. Finally, a viral vector-based transformation approach demonstrated that MdCOP1s negatively regulate the peel coloration of apple fruits by modulating the degradation of the MdMYB1 protein. Our findings provide new insight into the mechanism by which light controls anthocyanin accumulation and red fruit coloration in apple and even other plant species. PMID:22855936

  7. 76 FR 71374 - Joseph Giacchino, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-17

    ... does business. See Scott Sandarg, DMD, 74 FR 17528 (DEA 2009); David W. Wang, M.D., 72 FR 54297 (DEA... hearing, at which he may ultimately prevail. See, e.g., Bourne Pharmacy, 72 FR 18,273, 18,274 (2007); Agostino Carlucci, M.D., 49 FR 33,184, 33,184-85 (1984). Rather, what matters--as DEA has repeatedly...

  8. Sustainable water recovery from oily wastewater via forward osmosis-membrane distillation (FO-MD).

    PubMed

    Zhang, Sui; Wang, Peng; Fu, Xiuzhu; Chung, Tai-Shung

    2014-04-01

    This study proposed and investigated a hybrid forward osmosis - membrane distillation (FO-MD) system for sustainable water recovery from oily wastewater by employing lab-fabricated FO and MD hollow fiber membranes. Stable oil-in-water emulsions of different concentrations with small droplet sizes (<1 μm) were firstly prepared and applied as the feed solution in the FO process. Fouling was immediately observed in the FO mode and was low on the cellulose triacetate (CTA) - based thin film composite (TFC) membranes. Moreover, slight increment of fouling was observed in the first few hours and the water flux was then stabilized over 24 h. The characterizations of water flux and solute rejection in separate FO and MD processes revealed that a high water flux, good NaCl rejection, impressively high retention of oil droplets and partial permeation of acetic acid could be achieved. Finally, an integrated FO-MD system was developed to treat the oily wastewater containing petroleum, surfactant, NaCl and acetic acid at 60 °C in the batch mode. The water flux in FO undergoes three-stage decline due to fouling and reduction in osmotic driving force, but is quite stable in MD regardless of salt concentration. Oily wastewater with relatively high salinity could be effectively recovered by the FO-MD hybrid system while maintaining large water flux, at least 90% feed water recovery could be readily attained with only trace amounts of oil and salts, and the draw solution was re-generated for the next rounds of FO-MD run. Interestingly, significant amount of acetic acid was also retained in the permeate for further reuse as a chemical additive during the production of crude oil. The work has demonstrated that not only water but also organic additives in the wastewater could be effectively recovered by FO-MD systems for reuse or other utilizations. PMID:24463175

  9. Sustainable water recovery from oily wastewater via forward osmosis-membrane distillation (FO-MD).

    PubMed

    Zhang, Sui; Wang, Peng; Fu, Xiuzhu; Chung, Tai-Shung

    2014-04-01

    This study proposed and investigated a hybrid forward osmosis - membrane distillation (FO-MD) system for sustainable water recovery from oily wastewater by employing lab-fabricated FO and MD hollow fiber membranes. Stable oil-in-water emulsions of different concentrations with small droplet sizes (<1 μm) were firstly prepared and applied as the feed solution in the FO process. Fouling was immediately observed in the FO mode and was low on the cellulose triacetate (CTA) - based thin film composite (TFC) membranes. Moreover, slight increment of fouling was observed in the first few hours and the water flux was then stabilized over 24 h. The characterizations of water flux and solute rejection in separate FO and MD processes revealed that a high water flux, good NaCl rejection, impressively high retention of oil droplets and partial permeation of acetic acid could be achieved. Finally, an integrated FO-MD system was developed to treat the oily wastewater containing petroleum, surfactant, NaCl and acetic acid at 60 °C in the batch mode. The water flux in FO undergoes three-stage decline due to fouling and reduction in osmotic driving force, but is quite stable in MD regardless of salt concentration. Oily wastewater with relatively high salinity could be effectively recovered by the FO-MD hybrid system while maintaining large water flux, at least 90% feed water recovery could be readily attained with only trace amounts of oil and salts, and the draw solution was re-generated for the next rounds of FO-MD run. Interestingly, significant amount of acetic acid was also retained in the permeate for further reuse as a chemical additive during the production of crude oil. The work has demonstrated that not only water but also organic additives in the wastewater could be effectively recovered by FO-MD systems for reuse or other utilizations.

  10. Parallel Cascade Selection Molecular Dynamics (PaCS-MD) to generate conformational transition pathway.

    PubMed

    Harada, Ryuhei; Kitao, Akio

    2013-07-21

    Parallel Cascade Selection Molecular Dynamics (PaCS-MD) is proposed as a molecular simulation method to generate conformational transition pathway under the condition that a set of "reactant" and "product" structures is known a priori. In PaCS-MD, the cycle of short multiple independent molecular dynamics simulations and selection of the structures close to the product structure for the next cycle are repeated until the simulated structures move sufficiently close to the product. Folding of 10-residue mini-protein chignolin from the extended to native structures and open-close conformational transition of T4 lysozyme were investigated by PaCS-MD. In both cases, tens of cycles of 100-ps MD were sufficient to reach the product structures, indicating the efficient generation of conformational transition pathway in PaCS-MD with a series of conventional MD without additional external biases. Using the snapshots along the pathway as the initial coordinates, free energy landscapes were calculated by the combination with multiple independent umbrella samplings to statistically elucidate the conformational transition pathways.

  11. Imaging algorithm of a hand-held GPR MD sensor system (ALIS)

    NASA Astrophysics Data System (ADS)

    Feng, Xuan; Fujiwara, Jun; Zhou, Zheng-shu; Kobayashi, Takao; Sato, Motoyuki

    2005-06-01

    We are developing a new landmine detection system, called advanced landmine imaging system (ALIS), which is equipped with metal detector (MD) and ground penetrating radar (GPR). Although this is a hand-held system, we can record the MD and GPR signal with the sensor position information acquired by CCD camera. Therefore, 2D MD image and 3D GPR image are possible after signal processing. But because ALIS is a hand-held system, the sensor position is random when it is operated in the field. So interpolation processing is used to deal with the problem and offer grid data set for both MD and GPR. Good MD image can be achieved after interpolation. Also, interpolation can prepare good data set for migration to get good horizontal slice image. After interpolation, 3D diffraction stacking migration with migration aperture is used to refocus the scattered signals and enhance the signal-clutter ratio for reconstructed good GPR image. The ALIS was tested in Afghanistan in December 2004 and could achieve good landmine image. Especially, GPR could obtain good image of anti-person (AP) mine buried at more than 20cm depth. Also MD image and GPR image could combine to distinguish mine from metal fragment.

  12. Comparative Global Gene Expression Profiling Between rMd5- and rMd5deltaMeq-Infected Chickens: Host-Pathogen Interaction

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Marek’s disease virus (MDV), an oncogenic alpha-herpesvirus, is the etiological agent of Marek’s disease (MD), a highly contagious lymphoproliferative disease of domestic chickens. MDV encodes a basic leucine zipper protein, meq (MDV Eco Q), which is homologous to the Jun/Fos family of transcriptio...

  13. Cell lines, Md108 and Md66, from the hemocytes of Malacosoma disstria (Lepidoptera) display aspects of plasma-free innate non-self activities.

    PubMed

    Lapointe, Jason F; Dunphy, Gary B; Giannoulis, Paschalis; Mandato, Craig A; Nardi, James B; Gharib, Osama H; Niven, Donald F

    2011-11-01

    The innate non-self response systems of the deciduous tree pest, the forest tent caterpillar, Malacosoma disstria has been documented by us in terms of in vitro and in vivo reactions towards the Gram-positive nonpathogenic bacterium, Bacillus subtilis and Gram-negative pathogenic microbe, Xenorhabdus nematophila and their respective surface antigens, lipopoteichoic acids (LTA) and lipopolysaccharides (LPS). These studies, often conducted in whole and diluted hemolymph, preclude examination of plasma-free cellular (hemocyte) responses. Plasma-free hemocytes as primary cultures are difficult to obtain. The floating cell line Md66 and attached cell line Md108 from M. disstria hemocytes were examined as a model for plasma-free M. disstria hemocyte non-self responses. Herein, it was established that although both lines differed from each other and from the primary hemocyte cultures of M. disstria in growth parameters, cell composition and sizes both cell lines displayed granular cell-like (GL) cells and plasmatocyte-like (PL) cells according to morphological criteria and to some extent antigenic similarities based on labeling with anti-Chrysodeixis includens hemocyte monoclonal antibodies. Hemocyte-specific neuroglian-like protein was detected on cells of both cell lines and in the primary hemocyte cultures albeit with staining patterns differing according to culture and cell types, confluency levels and cell-cell adhesion. Both cell lines bound B. subtilis and X. nematophila, the reaction extent varying with the cell line and its cell types. LPS damaged both cell types in the two cell lines whereas LTA enhanced the adhesion of Md66 GL cells to flask surfaces followed by PL cell adhesion. PL cells of both lines, like the primary cultures, phagocytosed FITC-labeled B. subtilis; only Md108 GL cells phagocytosed B. subtilis. In either case phagocytosis was always less in frequency and intensity than the primary cultures. Proteins released from the cell lines differed in

  14. Novel matrine derivative MD-1 attenuates hepatic fibrosis by inhibiting EGFR activation of hepatic stellate cells.

    PubMed

    Feng, Yi; Ying, Hai-Yan; Qu, Ying; Cai, Xiao-Bo; Xu, Ming-Yi; Lu, Lun-Gen

    2016-09-01

    Matrine (MT), the effective component of Sophora flavescens Ait, has been shown to have anti-inflammation, immune-suppressive, anti-tumor, and anti-hepatic fibrosis activities. However, the pharmacological effects of MT still need to be strengthened due to its relatively low efficacy and short half-life. In the present study, we report a more effective thio derivative of MT, MD-1, and its inhibitory effects on the activation of hepatic stellate cells (HSCs) in both cell culture and animal models. Cytological experiments showed that MD-1 can inhibit the proliferation of HSC-T6 cells with a half-maximal inhibitory concentration (IC50) of 62 μmol/L. In addition, MD-1 more strongly inhibits the migration of HSC-T6 cells compared to MT and can more effectively induce G0/G1 arrest and apoptosis. Investigating the biological mechanisms underlying anti-hepatic fibrosis in the presence of MD-1, we found that MD-1 can bind the epidermal growth factor receptor (EGFR) on the surface of HSC-T6 cells, which can further inhibit the phosphorylation of EGFR and its downstream protein kinase B (Akt), resulting in decreased expression of cyclin D1 and eventual inhibition of the activation of HSC-T6 cells. Furthermore, in rats with dimethylnitrosamine (DMN)-induced hepatic fibrosis, MD-1 slowed the development and progression of hepatic fibrosis, protecting hepatic parenchymal cells and improving hepatic functions. Therefore, MD-1 is a potential drug for anti-hepatic fibrosis.

  15. TLR4/MD-2 activation by a synthetic agonist with no similarity to LPS

    PubMed Central

    Wang, Ying; Su, Lijing; Morin, Matthew D.; Jones, Brian T.; Whitby, Landon R.; Surakattula, Murali M. R. P.; Huang, Hua; Shi, Hexin; Choi, Jin Huk; Wang, Kuan-wen; Moresco, Eva Marie Y.; Berger, Michael; Zhan, Xiaoming; Zhang, Hong; Boger, Dale L.; Beutler, Bruce

    2016-01-01

    Structurally disparate molecules reportedly engage and activate Toll-like receptor (TLR) 4 and other TLRs, yet the interactions that mediate binding and activation by dissimilar ligands remain unknown. We describe Neoseptins, chemically synthesized peptidomimetics that bear no structural similarity to the established TLR4 ligand, lipopolysaccharide (LPS), but productively engage the mouse TLR4 (mTLR4)/myeloid differentiation factor 2 (MD-2) complex. Neoseptin-3 activates mTLR4/MD-2 independently of CD14 and triggers canonical myeloid differentiation primary response gene 88 (MyD88)- and Toll-interleukin 1 receptor (TIR) domain-containing adaptor inducing IFN-beta (TRIF)-dependent signaling. The crystal structure mTLR4/MD-2/Neoseptin-3 at 2.57-Å resolution reveals that Neoseptin-3 binds as an asymmetrical dimer within the hydrophobic pocket of MD-2, inducing an active receptor complex similar to that induced by lipid A. However, Neoseptin-3 and lipid A form dissimilar molecular contacts to achieve receptor activation; hence strong TLR4/MD-2 agonists need not mimic LPS. PMID:26831104

  16. PuReMD-GPU: A reactive molecular dynamics simulation package for GPUs

    SciTech Connect

    Kylasa, S.B.; Aktulga, H.M.; Grama, A.Y.

    2014-09-01

    We present an efficient and highly accurate GP-GPU implementation of our community code, PuReMD, for reactive molecular dynamics simulations using the ReaxFF force field. PuReMD and its incorporation into LAMMPS (Reax/C) is used by a large number of research groups worldwide for simulating diverse systems ranging from biomembranes to explosives (RDX) at atomistic level of detail. The sub-femtosecond time-steps associated with ReaxFF strongly motivate significant improvements to per-timestep simulation time through effective use of GPUs. This paper presents, in detail, the design and implementation of PuReMD-GPU, which enables ReaxFF simulations on GPUs, as well as various performance optimization techniques we developed to obtain high performance on state-of-the-art hardware. Comprehensive experiments on model systems (bulk water and amorphous silica) are presented to quantify the performance improvements achieved by PuReMD-GPU and to verify its accuracy. In particular, our experiments show up to 16× improvement in runtime compared to our highly optimized CPU-only single-core ReaxFF implementation. PuReMD-GPU is a unique production code, and is currently available on request from the authors.

  17. Chosen-Prefix Collisions for MD5 and Colliding X.509 Certificates for Different Identities

    NASA Astrophysics Data System (ADS)

    Stevens, Marc; Lenstra, Arjen; de Weger, Benne

    We present a novel, automated way to find differential paths for MD5. As an application we have shown how, at an approximate expected cost of 250 calls to the MD5 compression function, for any two chosen message prefixes P and P', suffixes S and S' can be constructed such that the concatenated values P||S and P'||S' collide under MD5. Although the practical attack potential of this construction of chosen-prefix collisions is limited, it is of greater concern than random collisions for MD5. To illustrate the practicality of our method, we constructed two MD5 based X.509 certificates with identical signatures but different public keys and different Distinguished Name fields, whereas our previous construction of colliding X.509 certificates required identical name fields. We speculate on other possibilities for abusing chosen-prefix collisions. More details than can be included here can be found on www.win.tue.nl/hashclash/ChosenPrefixCollisions/.

  18. An unusual dimeric structure and assembly for TLR4 regulator RP105-MD-1

    SciTech Connect

    Yoon, Sung-il; Hong, Minsun; Wilson, Ian A

    2011-11-16

    RP105-MD-1 modulates the TLR4-MD-2-mediated, innate immune response against bacterial lipopolysaccharide (LPS). The crystal structure of the bovine 1:1 RP105-MD-1 complex bound to a putative endogenous lipid at 2.9 Å resolution shares a similar overall architecture to its homolog TLR4-MD-2 but assembles into an unusual 2:2 homodimer that differs from any other known TLR-ligand assembly. The homodimer is assembled in a head-to-head orientation that juxtaposes the N-terminal leucine-rich repeats (LRRs) of the two RP105 chains, rather than the usual tail-to-tail configuration of C-terminal LRRs in ligand-activated TLR dimers, such as TLR1-TRL2, TLR2-TLR6, TLR3-TLR3 and TLR4-TLR4. Another unusual interaction is mediated by an RP105-specific asparagine-linked glycan, which wedges MD-1 into the co-receptor binding concavity on RP105. This unique mode of assembly represents a new paradigm for TLR complexes and suggests a molecular mechanism for regulating LPS responses.

  19. The protective efficacy of rMd5deltaMeq against challenge with a very virulent plus strain of MDV in relatively resistant lines of chickens

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Marek’s disease (MD) is a T-cell lymphoma of domestic chickens induced by MD virus (MDV), a naturally oncogenic and highly contagious cell-associated alpha-herpesvirus. A recombinant MDV lacking both copies of Meq oncogene, rMd5deltaMeq, was tested as a candidate vaccine against MD in a series of 19...

  20. An endophytic taxol-producing fungus from Taxus x media, Aspergillus candidus MD3.

    PubMed

    Zhang, Peng; Zhou, Peng-Peng; Yu, Long-Jiang

    2009-04-01

    An endophytic taxol-producing fungus (strain MD3) isolated from the inner bark of Taxus x media was identified as Aspergillus candidus according to its morphological characteristics, physiological and biochemical characteristics, and 18S rRNA gene sequence analysis. Taxol produced by A. candidus MD3 was shown to be identical to authentic taxol analyzed by UV, HPLC, MS and nuclear magnetic resonance spectroscopy. The gene encoding the 10-deacetylbaccatin III-10-O-acetyl transferase, which catalyzes formation of the last diterpene intermediate in the taxol biosynthetic pathway, has been cloned from A. candidus MD3 for the first time and possesses high homology to the same gene found in Taxus spp.

  1. MEGA-MD: molecular evolutionary genetics analysis software with mutational diagnosis of amino acid variation.

    PubMed

    Stecher, Glen; Liu, Li; Sanderford, Maxwell; Peterson, Daniel; Tamura, Koichiro; Kumar, Sudhir

    2014-05-01

    Computational diagnosis of amino acid variants in the human exome is the first step in assessing the disruptive impacts of non-synonymous single nucleotide variants (nsSNVs) on human health and disease. The Molecular Evolutionary Genetics Analysis software with mutational diagnosis (MEGA-MD) is a suite of tools developed to forecast the deleteriousness of nsSNVs using multiple methods and to explore nsSNVs in the context of the variability permitted in the long-term evolution of the affected position. In its graphical interface for use on desktops, it enables interactive computational diagnosis and evolutionary exploration of nsSNVs. As a web service, MEGA-MD is suitable for diagnosing variants on an exome scale. The MEGA-MD suite intends to serve the needs for conducting low- and high-throughput analysis of nsSNVs in diverse applications.

  2. The Emerging Physician-Scientist Workforce: Demographic, Experiential, and Attitudinal Predictors of MD-PhD Program Enrollment

    PubMed Central

    Jeffe, Donna B.; Andriole, Dorothy A.; Wathington, Heather D.; Tai, Robert H.

    2014-01-01

    Purpose MD-PhD scientists are a very successful, but small and fairly homogenous group of biomedical researchers. The authors conducted a retrospective cohort study to identify predictors of MD-PhD program enrollment to inform the development of evidence-based strategies to increase the size and diversity of the biomedical research workforce. Method Using de-identified data from all 2001–2006 Pre-Medical College Admission Test Questionnaire (PMQ) respondents, the authors developed multivariate logistic regression models to identify the demographic, experiential, and attitudinal variables associated with MD-PhD program enrollment at matriculation compared with all other MD program enrollment at matriculation and with not enrolling in medical school by August 2012. Results Of 207,436 PMQ respondents with complete data for all variables of interest, 2,575 (1.2%) were MD-PhD program enrollees, 80,856 (39.0%) were other MD program enrollees, and 124,005 (59.8%) were non-medical school matriculants. Respondents who were black (vs. white), high school and college laboratory research apprenticeship participants, and highly endorsed the importance of research/finding cures as reasons to study medicine were more likely to be MD-PhD program enrollees, whereas respondents who highly endorsed the status of medicine as a reason to study medicine were less likely to be MD-PhD program enrollees than either other MD program enrollees or non-medical school matriculants. Conclusions MD-PhD program directors appear to be successful in enrolling students whose attitudes and interests align with MD-PhD program goals. Continued efforts are needed to promote MD-PhD workforce diversity and the value of both high school and college research apprenticeships for students considering careers as physician-scientists. PMID:25006709

  3. Computation of shear viscosity of colloidal suspensions by SRD-MD.

    PubMed

    Laganapan, A M K; Videcoq, A; Bienia, M; Ala-Nissila, T; Bochicchio, D; Ferrando, R

    2015-04-14

    The behaviour of sheared colloidal suspensions with full hydrodynamic interactions (HIs) is numerically studied. To this end, we use the hybrid stochastic rotation dynamics-molecular dynamics (SRD-MD) method. The shear viscosity of colloidal suspensions is computed for different volume fractions, both for dilute and concentrated cases. We verify that HIs help in the collisions and the streaming of colloidal particles, thereby increasing the overall shear viscosity of the suspension. Our results show a good agreement with known experimental, theoretical, and numerical studies. This work demonstrates the ability of SRD-MD to successfully simulate transport coefficients that require correct modelling of HIs.

  4. Simulating picosecond X-ray diffraction from crystals using FFT methods on MD output

    SciTech Connect

    Kimminau, Giles; Nagler, Bob; Higginbotham, Andrew; Murphy, William; Wark, Justin; Park, Nigel; Hawreliak, James; Kalantar, Dan; Lorenzana, Hector; Remington, Bruce

    2007-12-12

    Multi-million atom non-equilibrium molecular dynamics (MD) simulations give significant insight into the transient processes that occur under shock compression. Picosecond X-ray diffraction enables the probing of materials on a timescale fast enough to test such effects. In order to simulate diffraction patterns, Fourier methods are required to gain a picture of reciprocal lattice space. We present here results of fast Fourier transforms of atomic coordinates of shocked crystals simulated by MD, and comment on the computing power required as a function of problem size. The relationship between reciprocal space and particular experimental geometries is discussed.

  5. Computation of shear viscosity of colloidal suspensions by SRD-MD

    SciTech Connect

    Laganapan, A. M. K.; Videcoq, A. Bienia, M.; Ala-Nissila, T.; Bochicchio, D.; Ferrando, R.

    2015-04-14

    The behaviour of sheared colloidal suspensions with full hydrodynamic interactions (HIs) is numerically studied. To this end, we use the hybrid stochastic rotation dynamics-molecular dynamics (SRD-MD) method. The shear viscosity of colloidal suspensions is computed for different volume fractions, both for dilute and concentrated cases. We verify that HIs help in the collisions and the streaming of colloidal particles, thereby increasing the overall shear viscosity of the suspension. Our results show a good agreement with known experimental, theoretical, and numerical studies. This work demonstrates the ability of SRD-MD to successfully simulate transport coefficients that require correct modelling of HIs.

  6. Study on the structure of Bayer liquor with spectroscopy and MD simulation

    NASA Astrophysics Data System (ADS)

    Chen, Yun; Feng, Qiming; Liu, Kun; Chen, Yuandao; Zhang, Guofan

    2006-05-01

    The combination of spectroscopy analysis (Raman and IR) and MD simulation was applied to explore the structure characters of Bayer liquor. In this work, MD simulation had been used to probe the nature of sodium metal-aluminate ion pairing and its role in the stabilization of clusters. It was found that the maximum concentration of Al(OH)4- occurs in low caustic solutions, but in high caustic solution, the appearance of Al(OH)63- was found. Results of molecular dynamic simulation indicated that the formation of clustering of aluminates in solution, and the clusters stabilized by sodium ions made a contribution to the formation of polyaluminate.

  7. Identification of Evolutionarily Conserved Md1 Splice Variants That Regulate Innate Immunity through Differential Induction of NF-кB.

    PubMed

    Candel, Sergio; Tyrkalska, Sylwia D; García-Moreno, Diana; Meseguer, José; Mulero, Victoriano

    2016-08-15

    Although in mammals the TLR4/myeloid differentiation factor (MD)2/CD14 complex is responsible for the recognition of bacterial LPS, and it is known that the RP105/MD1 complex negatively regulates TLR4 signaling, the evolutionary history of LPS recognition remains enigmatic. Thus, zebrafish has orthologs of mammalian TLR4 (Tlr4a and Tlr4b), RP105, and MD1, but MD2 and CD14 seem to be absent from all fish genomes available to date. In addition, and to make the story more intriguing, zebrafish Tlr4a and Tlr4b do not recognize LPS, whereas the zebrafish Rp105/Md1 complex unexpectedly participates in the regulation of innate immunity and viral resistance. In this work, we report the identification of two novel splice variants of Md1, which are expressed at similar levels as full-length Md1 in the main immune-related organs of zebrafish and are highly induced upon viral infection. One of these splice variants, which is also expressed by mouse macrophages, lacks three conserved cysteine residues that have been shown to form disulfide bonds that are crucial for the three-dimensional structure of the MD-2-related lipid recognition domain of Md1. Functional studies in zebrafish demonstrate that this evolutionarily conserved splice variant shows higher antiviral activity than full-length Md1, but reduced proinflammatory activity, due to an impaired ability to activate the master regulator of inflammation, NF-κB. These results uncover a previously unappreciated evolutionarily conserved Md1 splice variant with important functions in the regulation of innate immunity and the antiviral response in zebrafish, and point to the need for additional functional studies in mammals on this little explored molecule.

  8. 76 FR 71371 - James L. Hooper, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-17

    ...); Shatz v. U.S. Dept. of Justice, 873 F.2d 1089, 1091 (8th Cir. 1989); Thomas E. Johnston, 45 FR 72311... decision in Anne Lazar Thorn, M.D., 62 FR 12847 (1997), stands for the proposition that the Agency's... this case. Id. at 2 (quoting 62 FR at 12848). Notwithstanding the implication of the above passage,...

  9. 78 FR 62678 - Morris W. Cochran, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ... Specialty Pharmacy, 76 FR 66,965 (DEA 2011); Jesus R. Juarez, M.D., 62 FR 14,945 (DEA 1997); Dominick A... action.'' R.D. at 11 (citing Kamal Tiwari, 76 FR 76 FR 71604, 71605 (2011)). This is an... finding that the practitioner's federal registration had been suspended. See 76 FR at 71606...

  10. 76 FR 80230 - Amendment of Class D and E Airspace; Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-23

    ... FR 72837). This action further corrects the geographic coordinates to be in concert with the FAAs..., 2011, 76 FR 72837, FR Doc. 2011-30489, are corrected as follows: AEA MD D Baltimore, Martin State... the geographic coordinates in the airspace description of a final rule published in the...

  11. 76 FR 72837 - Amendment of Class D and Class E Airspace; Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ..., Baltimore, MD (76 FR 54153). Interested parties were invited to participate in this rulemaking effort by... Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p....

  12. 76 FR 65118 - Drawbridge Operation Regulation; Bear Creek, Sparrows Point, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-20

    ... SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulation; Bear Creek, Sparrows... Avenue) highway toll drawbridge across Bear Creek, mile 1.5, Sparrows Point, MD was replaced with a fixed... Bear Creek, mile 1.5 was removed and replaced with a fixed bridge in 1998. Prior to 1998, a...

  13. 75 FR 27430 - Special Local Regulations for Marine Events; Patapsco River, Northwest Harbor, Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-17

    ...; Patapsco River, Northwest Harbor, Baltimore, MD'' in the Federal Register (75 FR 16374). We received no...: The Coast Guard is establishing special local regulations during the ``Baltimore Dragon Boat Challenge... June 19, 2010, Baltimore Dragon Boat Club, Inc. will sponsor Dragon Boat Races in the Patapsco...

  14. 75 FR 70126 - Safety Zone; Fireworks Displays, Potomac River, National Harbor, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ..., Potomac River, National Harbor, MD'' in the Federal Register (75 FR 169). We received one comment on the... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Fireworks Displays, Potomac River, National... the safety of life on navigable waters during fireworks displays launched from a discharge...

  15. 76 FR 77580 - Maryland Disaster #MD-00020 Declaration of Economic Injury

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-13

    ... ADMINISTRATION Maryland Disaster MD-00020 Declaration of Economic Injury AGENCY: U.S. Small Business...: Submit completed loan applications to: U.S. Small Business Administration, Processing and Disbursement... of Disaster Assistance, U.S. Small Business Administration, 409 3rd Street SW., Suite...

  16. Crystal twinning of human MD-2 recognizing endotoxin cores of lipopolysaccharide.

    PubMed

    Ohto, Umeharu; Satow, Yoshinori

    2008-05-01

    Twinning of crystals causes overlapping of two or more reciprocal lattice points, and hence structure amplitudes for a single crystalline domain are hardly obtained from X-ray diffraction intensities. MD-2 protein forms a stable complex with Toll-like receptor 4 and recognizes bacterial lipopolysaccharide (LPS). Excessive immune responses activated by LPS cause septic shocks. Saccharide-trimmed human MD-2 crystallizes in the tetragonal form with apparent Laue symmetry of 4/mmm, and diffraction intensities from these crystals indicate crystal twinning. The crystal consists of two different domains, A and B. The c(A) axis of domain A coincides with the c(B) axis of domain B with a smaller lattice, and the a(A) axis corresponds to the (a(B) + b(B)) axis. This twinning severely imposes difficulty in structure determination. Through optimization of cryoprotectant, domain A was thoroughly transformed into domain B. The crystal containing only domain B is in space group P4(1)2(1)2 with one MD-2 molecule in the asymmetric unit. The structure of this form of MD-2 as well as its complex with antiendotoxic lipid IVa was successfully determined using the multiple isomorphous replacement method. PMID:18421154

  17. 76 FR 60873 - Jeffery M. Freesemann, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-30

    ... Enforcement Administration Jeffery M. Freesemann, M.D.; Decision and Order On January 24, 2011, Administrative Law Judge (ALJ) John J. Mulrooney, II, issued the attached recommended decision. The Respondent did not file exceptions to the decision. Having considered the ALJ's decision and the record in light...

  18. 77 FR 57133 - T.J. Mcnichol, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... making the initial decision * * *'')). Because even assuming, without deciding, that the Expert's... Enforcement Administration T.J. Mcnichol, M.D.; Decision and Order On October 27, 2011, I, the Administrator... issued his recommended decision. With respect to factor one--the recommendation of the state...

  19. 76 FR 16823 - Robert L. Dougherty, M.D.; Denial of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ... prescribing of controlled substances to three patients. Id. (citing 60 FR 55047). More specifically, the Show... treatment record of Patient 1 could have ultimately jeopardized that patient's welfare.'' 60 FR at 55051. \\2... previously held a DEA Certificate of Registration as a practitioner. Robert L. Dougherty, Jr., M.D., 60...

  20. 76 FR 21406 - Cynthia M. Cadet, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF JUSTICE Drug Enforcement Administration Cynthia M. Cadet, M.D.; Decision and Order ACTION: Correction. On Thursday, April 7, 2011, the Drug Enforcement Administration published the above-titled Decision and Order, as well as...

  1. Ab Initio ONIOM-Molecular Dynamics (MD) Study on the Deamination Reaction by Cytidine Deaminase

    SciTech Connect

    Matsubara, Toshiaki; Dupuis, Michel; Aida, Misako

    2007-08-23

    We applied the ONIOM-molecular dynamics (MD) method to the hydrolytic deamination of cytidine by cytidine deaminase, which is an essential step of the activation process of the anticancer drug inside the human body. The direct MD simulations were performed for the realistic model of cytidine deaminase calculating the energy and its gradient by the ab initio ONIOM method on the fly. The ONIOM-MD calculations including the thermal motion show that the neighboring amino acid residue is an important factor of the environmental effects and significantly affects not only the geometry and energy of the substrate trapped in the pocket of the active site but also the elementary step of the catalytic reaction. We successfully simulate the second half of the catalytic cycle, which has been considered to involve the rate-determining step, and reveal that the rate-determing step is the release of the NH3 molecule. TM and MA were supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan. MD was supported by the Division of Chemical Sciences, Office of Basic Energy Sciences, and by the Office of Biological and Environmental Research of the U.S. Department of Energy DOE. Battelle operates Pacific Northwest National Laboratory for DOE.

  2. 76 FR 66968 - Abelardo E. Lecompte-Torres, M.D. Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-28

    ... registration. Steven B. Brown, 75 FR 65660, 65663 (2010) (citing John B. Freitas, 74 FR 17524, 17525 (2009)); Dominick A. Ricci, 58 FR 51104, 51105 (1993); Bobby Watts, 53 FR 11919, 11920 (1988). It is undisputed that... Enforcement Administration Abelardo E. Lecompte-Torres, M.D. Decision and Order On April 29, 2010, the...

  3. Large-scale MD simulations investigating H plasma interactions with Tungsten surfaces

    NASA Astrophysics Data System (ADS)

    Cusentino, Mary Alice; Wirth, Brian

    2015-11-01

    Tungsten is a prime candidate material for the divertor in future fusion reactors such as ITER. However, the tungsten divertor will need to be able to withstand high fluxes, on the order of 1024m-2s-1, of low energy hydrogen. It is crucial to understand both the tungsten surface response as well as the hydrogen retention and recycling for the divertor region. Molecular dynamics (MD) is a useful tool to study these effects. One issue with MD is that implantation fluxes tend to be very high, on the order of 1027 m-2s-1, due to time and computational limitations. By performing large scale MD on supercomputers, it is possible to reach more realistic fluxes of 1025 m-2s-1. Results will be presented from MD simulations from a 50 nm x 50 nm x 25 nm tungsten box at 1200 K and 2000 K. Hydrogen is implanted every 10 ps based on the 60 eV depth distribution calculated by SRIM, which amounts to a flux of 4 x 1025 m-2s-1. A modified version of the Juslin bond order W-H potential is used to describe the W-H interactions. Preliminary results show an initially high retention of hydrogen that accumulates in a sub-surface region. These simulations provide insight into the early stages of surface deformation as well as hydrogen retention for the tungsten divertor.

  4. James McHenry, M.D. of Fort McHenry in Baltimore Towne.

    PubMed

    Miller, J M

    1992-05-01

    James McHenry, M.D., whose name is best known for the fort immortalized in the Star Spangled Banner, was one of the early members of Med Chi. In addition, he was secretary to George Washington; aide to Lafayette; member of the Maryland Senate, the General Assembly of Maryland, and the US Congress; and signer of the Constitution.

  5. PERSONAL EXPOSURE TO PM2.5 MASS AND TRACE ELEMENTS IN BALTIMORE, MD, USA

    EPA Science Inventory

    In a recent study, EPA found significant relationships between PM2.5 mass measurements at a community site and personal exposure samples in a Towson, MD retirement facility. This manuscript builds upon these results by evaluating the exposure relationships with the elemental c...

  6. Vienna-PTM web server: a toolkit for MD simulations of protein post-translational modifications.

    PubMed

    Margreitter, Christian; Petrov, Drazen; Zagrovic, Bojan

    2013-07-01

    Post-translational modifications (PTMs) play a key role in numerous cellular processes by directly affecting structure, dynamics and interaction networks of target proteins. Despite their importance, our understanding of protein PTMs at the atomistic level is still largely incomplete. Molecular dynamics (MD) simulations, which provide high-resolution insight into biomolecular function and underlying mechanisms, are in principle ideally suited to tackle this problem. However, because of the challenges associated with the development of novel MD parameters and a general lack of suitable computational tools for incorporating PTMs in target protein structures, MD simulations of post-translationally modified proteins have historically lagged significantly behind the studies of unmodified proteins. Here, we present Vienna-PTM web server (http://vienna-ptm.univie.ac.at), a platform for automated introduction of PTMs of choice to protein 3D structures (PDB files) in a user-friendly visual environment. With 256 different enzymatic and non-enzymatic PTMs available, the server performs geometrically realistic introduction of modifications at sites of interests, as well as subsequent energy minimization. Finally, the server makes available force field parameters and input files needed to run MD simulations of modified proteins within the framework of the widely used GROMOS 54A7 and 45A3 force fields and GROMACS simulation package. PMID:23703210

  7. Vienna-PTM web server: a toolkit for MD simulations of protein post-translational modifications

    PubMed Central

    Margreitter, Christian; Petrov, Drazen; Zagrovic, Bojan

    2013-01-01

    Post-translational modifications (PTMs) play a key role in numerous cellular processes by directly affecting structure, dynamics and interaction networks of target proteins. Despite their importance, our understanding of protein PTMs at the atomistic level is still largely incomplete. Molecular dynamics (MD) simulations, which provide high-resolution insight into biomolecular function and underlying mechanisms, are in principle ideally suited to tackle this problem. However, because of the challenges associated with the development of novel MD parameters and a general lack of suitable computational tools for incorporating PTMs in target protein structures, MD simulations of post-translationally modified proteins have historically lagged significantly behind the studies of unmodified proteins. Here, we present Vienna-PTM web server (http://vienna-ptm.univie.ac.at), a platform for automated introduction of PTMs of choice to protein 3D structures (PDB files) in a user-friendly visual environment. With 256 different enzymatic and non-enzymatic PTMs available, the server performs geometrically realistic introduction of modifications at sites of interests, as well as subsequent energy minimization. Finally, the server makes available force field parameters and input files needed to run MD simulations of modified proteins within the framework of the widely used GROMOS 54A7 and 45A3 force fields and GROMACS simulation package. PMID:23703210

  8. THE NATIONAL COASTAL ASSESSMENT SETAC ANNUAL MEETING, BALTIMORE, MD NOV 2001

    EPA Science Inventory

    To be presented at the 22nd Annual Meeting of the Society of Environmental Toxicology and Chemistry: Changing Environmental Awareness: Societal Concerns and Scientific Responses, 11-15 November 2001, Baltimore, MD. 1 p. (ERL,GB R859).

    The primary purpose of the Environmen...

  9. PHYSICAL PROPERTIES OF NEAR-EARTH ASTEROID 2011 MD

    SciTech Connect

    Mommert, M.; Trilling, D. E.; Farnocchia, D.; Chesley, S. R.; Chodas, P. W.; Hora, J. L.; Smith, H. A.; Fazio, G. G.; Mueller, M.; Harris, A. W.

    2014-07-01

    We report on observations of near-Earth asteroid 2011 MD with the Spitzer Space Telescope. We have spent 19.9 hr of observing time with channel 2 (4.5 μm) of the Infrared Array Camera and detected the target within the 2σ positional uncertainty ellipse. Using an asteroid thermophysical model and a model of nongravitational forces acting upon the object, we constrain the physical properties of 2011 MD, based on the measured flux density and available astrometry data. We estimate 2011 MD to be (6{sub −2}{sup +4}) m in diameter with a geometric albedo of 0.3{sub −0.2}{sup +0.4} (uncertainties are 1σ). We find the asteroid's most probable bulk density to be (1.1{sub −0.5}{sup +0.7}) g cm{sup –3}, which implies a total mass of (50-350) t and a macroporosity of ≥65%, assuming a material bulk density typical of non-primitive meteorite materials. A high degree of macroporosity suggests that 2011 MD is a rubble-pile asteroid, the rotation of which is more likely to be retrograde than prograde.

  10. 75 FR 49992 - Peter W.S. Grigg, M.D.; Revocation of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-16

    ... * * * considered in the disjunctive.'' Robert A. Leslie, M.D., 68 FR 15227, 15230 (2003). I ``may rely on any one...). However, under Agency precedent, neither of these findings is dispositive. See Edmund Chein, 72 FR 6580, 6590 n.22 (2007); Mortimer B. Levin, 55 FR 8209, 8210 (1990). Moreover, the evidence with respect...

  11. Comments on ""Lake Woebegone," Twenty Years Later" by J. J. Cannell, MD

    ERIC Educational Resources Information Center

    McRae, D. J.

    2006-01-01

    This article presents the author's comments on ""Lake Woebegone," Twenty Years Later" by J. J. Cannell, MD. J. J. Cannell's article on the so-called "Lake Woebegone" effect for K-12 educational testing systems is mostly an historical account of technical issues and policy considerations that led in part to development of new types of test…

  12. 75 FR 36577 - Airworthiness Directives; McDonnell Douglas Corporation Model MD-90-30 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-28

    ...-07-04, Amendment 39-15863 (74 FR 14460, March 31, 2009), for certain Model MD-90-30 airplanes. That... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... Amendment 39-15863 (74 FR 14460, March 31, 2009) and adding the following new AD: McDonnell...

  13. 78 FR 32556 - Safety Zone; 2013 Ocean City Air Show, Atlantic Ocean; Ocean City, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; 2013 Ocean City Air Show, Atlantic Ocean... establishing a temporary safety zone on the navigable waters of the Atlantic Ocean in the vicinity of Ocean... Atlantic Ocean in Ocean City, MD. In recent years, there have been unfortunate instances of jets and...

  14. 76 FR 31235 - Safety Zone; Ocean City Air Show, Atlantic Ocean, Ocean City, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Ocean City Air Show, Atlantic Ocean, Ocean... establish a temporary safety zone on the Atlantic Ocean in the vicinity of Ocean City, MD to support the... Atlantic Ocean to protect mariners from the hazards associated with air show events. DATES: This rule...

  15. 76 FR 416 - CSX Transportation, Inc.-Abandonment Exemption-in Allegany County, Md.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-04

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Surface Transportation Board CSX Transportation, Inc.--Abandonment Exemption--in Allegany County, Md. AGENCY: Surface Transportation Board, DOT. ACTION: Notice of exemption. SUMMARY: Under 49 U.S.C....

  16. 75 FR 18056 - Safety Zone; Fireworks Display, Patuxent River, Solomons Island Harbor, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-09

    ... Island Harbor, MD AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone upon specified waters of Solomons Island Harbor, a tributary of the... fireworks display launched from discharge barge located in Solomons Island, Calvert County, Maryland....

  17. Electrochemical endotoxin sensors based on TLR4/MD-2 complexes immobilized on gold electrodes.

    PubMed

    Yeo, Tae Yun; Choi, Ji Suk; Lee, Byung Kook; Kim, Beob Soo; Yoon, Hwa In; Lee, Hyeong Yun; Cho, Yong Woo

    2011-10-15

    Even low concentrations of endotoxins can be life-threatening. As such, continuous effort has been directed toward the development of sensitive and specific endotoxin detection systems. In this paper, we report the design and fabrication of a new electrochemical endotoxin sensor based on a human recombinant toll-like receptor 4 (rhTLR4) and myeloid differentiation-2 (MD-2) complex. The rhTLR4/MD-2 complex, which specifically binds to endotoxin, was immobilized on gold electrodes through a self-assembled monolayer (SAM) technique involving the use of dithiobis(succinimidyl undecanoate) (DSU). The surface topography of the electrodes at each fabrication stage was characterized with a nanosurface profiler and atomic force microscope (AFM). The electrochemical signals generated from interactions between the rhTLR4/MD-2 complex and the endotoxin were characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). A linear relationship between the peak current and endotoxin concentration was obtained in the range of 0.0005 to 5 EU/mL with a correlation coefficient (R(2)) of 0.978. The estimated limit of detection (LOD) was fairly low, 0.0002 EU/mL. The rhTLR4/MD-2 based sensors exhibited no current responses to dipalmitoylphosphatidylcholine (DPPC) bearing two lipid chains, which is structurally similar to endotoxin, indicating the high specificity of the sensors to endotoxin. PMID:21816600

  18. Genome Sequence of Chlamydia suis MD56, Isolated from the Conjunctiva of a Weaned Piglet

    PubMed Central

    Donati, Manuela; Huot-Creasy, Heather; Humphrys, Michael; Di Paolo, Maria; Di Francesco, Antonietta

    2014-01-01

    Chlamydia suis is a natural pathogen of pigs (Sus scrofa) and causes conjunctivitis, pneumonia, enteritis, and various reproductive disorders that adversely impact this economically important animal. Here, we report the first C. suis genome, that of C. suis MD56, isolated from a conjunctival swab of a weaned piglet. PMID:24812227

  19. 77 FR 45675 - Margy Temponeras, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-01

    ... Judge (ALJ) Timothy D. Wing issued the attached recommended decision. Neither party filed exceptions to... encompasses the factors listed in Section 824(a).'' ALJ at 19 n.24 (citing Kuen H. Chen, M.D., 58 FR 65401... 21 U.S.C. 824(a).'' 58 FR at 65402. However, whether this constitutes a holding or merely...

  20. 76 FR 21406 - Michael J. Aruta, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-15

    ... Decision of the Administrative Law Judge (76 FR 19420). In preparing the document for publication, the... at 76 FR 19420 under the third column. Dated: April 8, 2011. Michele M. Leonhart, Administrator... Enforcement Administration Michael J. Aruta, M.D.; Decision and Order ACTION: Correction. On Thursday, April...

  1. 76 FR 4940 - Algirdas J. Krisciunas, M.D.; Revocation of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-27

    .... Freitas, 74 FR 17524, 17525 (2009); Worth S. Wilkinson, 71 FR 30173 (2006); Stephen J. Graham, 69 FR 11661, 11662 (2004); Dominick A. Ricci, 58 FR 51104, 51105 (1993); Bobby Watts, 53 FR 11919, 11920 (1988). I... Enforcement Administration Algirdas J. Krisciunas, M.D.; Revocation of Registration On January 19, 2010,...

  2. 77 FR 29691 - Matthew J. Kachinas, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... practices medicine. See, e.g., Calvin Ramsey, 76 FR 20034, 20036 (2011); Dominick A. Ricci, 58 FR 51104, 51105 (1993); Bobby Watts, 53 FR 11919, 11920 (1988). As found above, on April 16, 2010, the Florida... Enforcement Administration Matthew J. Kachinas, M.D.; Decision and Order On September 27, 2011, the...

  3. 75 FR 8465 - Airworthiness Directives; McDonnell Douglas Corporation Model MD-90-30 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... Aircraft Certification Office, 3960 Paramount Boulevard, Lakewood, California 90712-4137; telephone (562... Model MD-90-30 airplanes. That NPRM was published in the Federal Register on September 4, 2009 (74 FR... flight of civil aircraft in air commerce by prescribing regulations for practices, methods,...

  4. Novel plasmid conferring kanamycin and tetracycline resistance in turkey-derived Campylobacter jejuni strain 11601MD

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In Campylobacter spp., resistance to the antibiotics kanamycin and tetracycline is frequently associated with plasmid-borne genes. However, relatively few plasmids of Campylobacter jejuni have been fully characterized to date. A novel plasmid (p11601MD; 44,095 bp.) harboring tet(O) was identified in...

  5. 33 CFR 334.140 - Chesapeake Bay; U.S. Army Proving Ground Reservation, Aberdeen, Md.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Chesapeake Bay; U.S. Army Proving... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.140 Chesapeake Bay; U.S. Army Proving Ground Reservation, Aberdeen, Md. (a) Restricted area...

  6. 33 CFR 334.140 - Chesapeake Bay; U.S. Army Proving Ground Reservation, Aberdeen, Md.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Chesapeake Bay; U.S. Army Proving... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.140 Chesapeake Bay; U.S. Army Proving Ground Reservation, Aberdeen, Md. (a) Restricted area...

  7. 77 FR 64128 - Jose Gonzalo Zavaleta, M.D.; Denial of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-18

    ... of the first Show Cause Order. See Jose Gonzalo Zavaleta, M.D., 76 FR 49506 (Aug. 10, 2011). Therein...., Alexandria, Louisiana. 76 FR 49506. However, on March 26, 2008, concurrent with Applicant's arrest on state... allowed.'' 76 FR at 49506. Upon receipt of this information, an undercover state trooper (UC1)...

  8. 76 FR 20025 - Alan H. Olefsky, M.D.; Denial of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ... entirety and revoked Respondent's registration. GX 4, at 3 (57 FR 928 (1992)). The Administrator expressly.... Olefsky, M.D., 72 FR 42127 (2007) (GX 3B, at 1). Among the conditions imposed by the order were that....'' See 72 FR at 42128 (citing 21 U.S.C. 802(21), 823(f), and 843(a)(3)). Following his...

  9. 76 FR 35802 - Special Local Regulations for Marine Events; Patuxent River, Solomons, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a public meeting. But you may... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations for Marine Events; Patuxent... races, a marine event to be held on the waters of the Patuxent River, near Solomons, MD on September...

  10. 76 FR 52865 - Special Local Regulations for Marine Events; Patuxent River, Solomons, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-24

    ... Marine Events; Patuxent River, Solomons, MD'' in the Federal Register (76 FR 118). We received no... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations for Marine Events; Patuxent... establishing special local regulations during the ``Chesapeake Challenge'' power boat races, a marine event...

  11. 77 FR 47279 - Special Local Regulations for Marine Events, Patuxent River; Solomons, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice..., Solomons, MD'' in the Federal Register (77 FR 25649). We received no comments on the proposed rule. No... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations for Marine Events,...

  12. 75 FR 41789 - Special Local Regulations for Marine Events; Patuxent River, Solomons, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... for Marine Events; Patuxent River, Solomons, MD'' in the Federal Register (75 FR 111). The NPRM stated... public dockets in the January 17, 2008, issue of the Federal Register (73 FR 3316). Public Meeting We do... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations for Marine Events;...

  13. 75 FR 32866 - Special Local Regulations for Marine Events; Patuxent River, Solomons, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-10

    ... public dockets in the January 17, 2008, issue of the Federal Register (73 FR 3316). Public Meeting We do... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations for Marine Events; Patuxent... races, a marine event to be held on the waters of the Patuxent River, near Solomons, MD on October...

  14. 77 FR 25649 - Special Local Regulations for Marine Events; Patuxent River, Solomons, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-01

    ... notice regarding our public dockets in the January 17, 2008, issue of the Federal Register (73 FR 3316... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations for Marine Events; Patuxent... races, a marine event to be held on the waters of the Patuxent River, near Solomons, MD on September...

  15. 76 FR 51424 - Satinder Dang, M.D.; Revocation of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... continuation of a registration is consistent with the public interest.'' Id. (citing Patrick W. Stodola, 74 FR 20727, 20730 (2009); Jayam Krishna-Iyer, 74 FR 459, 461 (2009)). The ALJ then concluded that ``this... Respondent's registration.'' Id. (citing Harrell E. Robinson, M.D., 74 FR 61370, 61376-77 (2010)). The...

  16. 33 CFR 165.507 - Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Security Zone; Chesapeake Bay... Guard District § 165.507 Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD. (a... security zone: All waters of the Chesapeake Bay, from the surface to the bottom, within 250 yards north...

  17. 33 CFR 165.507 - Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Security Zone; Chesapeake Bay... Guard District § 165.507 Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD. (a... security zone: All waters of the Chesapeake Bay, from the surface to the bottom, within 250 yards north...

  18. 33 CFR 165.507 - Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Security Zone; Chesapeake Bay... Guard District § 165.507 Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD. (a... security zone: All waters of the Chesapeake Bay, from the surface to the bottom, within 250 yards north...

  19. 33 CFR 165.507 - Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Security Zone; Chesapeake Bay... Guard District § 165.507 Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD. (a... security zone: All waters of the Chesapeake Bay, from the surface to the bottom, within 250 yards north...

  20. 33 CFR 165.507 - Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Chesapeake Bay... Guard District § 165.507 Security Zone; Chesapeake Bay, between Sandy Point and Kent Island, MD. (a... security zone: All waters of the Chesapeake Bay, from the surface to the bottom, within 250 yards north...

  1. 76 FR 16821 - Gregory F. Saric, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ... similar to that of Respondent's.'' Exceptions at 1 (citing Stuart A. Bergman, M.D., 70 FR 33193 (2005... Health Servs., 72 FR 42126 (2007) (quoting Bourne Pharmacy, Inc., 72 FR 18273, 18274 (2007) (quoting 21 U... * * * controlled substances under the laws of the State in which he practices.''); Bourne Pharmacy, 72 FR at...

  2. 76 FR 70346 - Drawbridge Operation Regulation; Isle of Wight (Sinepuxent) Bay, Ocean City, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulation; Isle of Wight (Sinepuxent) Bay, Ocean City, MD AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast Guard is... their fields, and governmental jurisdictions with populations of less than 50,000. The Coast...

  3. The draft genome of MD-2 pineapple using hybrid error correction of long reads

    PubMed Central

    Redwan, Raimi M.; Saidin, Akzam; Kumar, S. Vijay

    2016-01-01

    The introduction of the elite pineapple variety, MD-2, has caused a significant market shift in the pineapple industry. Better productivity, overall increased in fruit quality and taste, resilience to chilled storage and resistance to internal browning are among the key advantages of the MD-2 as compared with its previous predecessor, the Smooth Cayenne. Here, we present the genome sequence of the MD-2 pineapple (Ananas comosus (L.) Merr.) by using the hybrid sequencing technology from two highly reputable platforms, i.e. the PacBio long sequencing reads and the accurate Illumina short reads. Our draft genome achieved 99.6% genome coverage with 27,017 predicted protein-coding genes while 45.21% of the genome was identified as repetitive elements. Furthermore, differential expression of ripening RNASeq library of pineapple fruits revealed ethylene-related transcripts, believed to be involved in regulating the process of non-climacteric pineapple fruit ripening. The MD-2 pineapple draft genome serves as an example of how a complex heterozygous genome is amenable to whole genome sequencing by using a hybrid technology that is both economical and accurate. The genome will make genomic applications more feasible as a medium to understand complex biological processes specific to pineapple. PMID:27374615

  4. 75 FR 68245 - Airworthiness Directives; McDonnell Douglas Corporation Model MD-90-30 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-05

    ... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; McDonnell Douglas...Donnell Douglas Model MD-90-30 airplanes. This proposed AD would require installing new fire...

  5. 75 FR 21528 - Airworthiness Directives; McDonnell Douglas Corporation Model MD-90-30 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-26

    ... Policies and Procedures (44 FR 11034, February 26, 1979), and 3. Will not have a significant economic... Douglas Corporation Model MD- 90-30 Airplanes AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... main landing gear (MLG) during gear extension, damaging the hydraulic system on McDonnell...

  6. 76 FR 21406 - Beau Boshers, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-15

    ... Decision of the Administrative Law Judge (76 FR 19401). In preparing the document for publication, the... at 76 FR 19404 under the third column. Dated: April 8, 2011. Michele M. Leonhart, Administrator... Enforcement Administration Beau Boshers, M.D.; Decision and Order ACTION: Correction. On Thursday, April...

  7. 76 FR 21406 - Jacobo Dreszer, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-15

    ... Decision of the Administrative Law Judge (76 FR 19386). In preparing the document for publication, the... at 76 FR 19390 under the first column. Dated: April 8, 2011. Michele M. Leonhart, Administrator... Enforcement Administration Jacobo Dreszer, M.D.; Decision and Order ACTION: Correction. On Thursday, April...

  8. 76 FR 21406 - Roni Dreszer, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-15

    ... Decision of the Administrative Law Judge (76 FR 19434). In preparing the document for publication, the... at 76 FR 19437 under the second column. Dated: April 8, 2011. Michele M. Leonhart, Administrator... Enforcement Administration Roni Dreszer, M.D.; Decision and Order ACTION: Correction. On Thursday, April...

  9. 77 FR 25592 - Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-01

    ...; Patapsco River, Northwest and Inner Harbors, Baltimore, MD'' in the Federal Register (77 FR 11434). We... Harbor during the movement of the historic sloop-of-war USS CONSTELLATION on May 24, 2012. This action is...-of-war USS CONSTELLATION in Baltimore, Maryland on May 24, 2012. Planned events include a three-...

  10. A mathematic model that describes modes of MdSGHV transmission within house fly populations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this paper it is proposed that one potential component by which the Musca domestica salivary gland hypertrophy virus (MdSGHV) infects individual flies is through cuticular damage. Breaks in the cuticle allow entry of the virus into the hemocoel causing the infection. Male flies typically have a h...

  11. 77 FR 2493 - Special Local Regulations for Marine Events; Spa Creek and Annapolis Harbor, Annapolis, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-18

    ... ``TriRock Triathlon Series'', a marine event to be held on the waters of Spa Creek and Annapolis Harbor...), U.S. Department of Transportation, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue... Houck, U.S. Coast Guard Sector Baltimore, MD; telephone (410) 576-2674, email...

  12. Prediction of nitroxide spin label EPR spectra from MD trajectories: application to myoglobin.

    PubMed

    Kuprusevicius, Egidijus; White, Gaye; Oganesyan, Vasily S

    2011-01-01

    We report the prediction of motional EPR spectra of the metalloprotein sperm whale myoglobin spin labelled with nitroxide directly from Molecular Dynamics (MD) simulations at the atomistic scale. We show that an accurate simulation of EPR spectra can be achieved from a single MD trajectory which is in excellent agreement with experiment. Simulations have been carried out using a general method reported previously by us for the simulation of EPR spectra form single dynamical trajectories. Our calculations demonstrate the complex nature of the dynamics of a spin label which is a superposition of the fast librational motions around dihedral states, of slow conformational flips among different rotameric states and of the slow rotational diffusion of the protein itself. The MD-EPR methodology reported does not require any additional stochastic modelling using adjustable parameters and opens, for the first time, the prospect of the simulation of EPR spectra entirely from single MD trajectories. Such a technique not only simplifies the interpretation and analysis of EPR spectra but also opens the possibility, for example, of "computer engineering" of spin-labelled proteins with the desired properties prior to actual EPR experiment. PMID:21322490

  13. 78 FR 42998 - CSX Transportation, Inc.-Abandonment Exemption-in Washington County, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-18

    ... Surface Transportation Board CSX Transportation, Inc.--Abandonment Exemption--in Washington County, MD On June 28, 2013, CSX Transportation, Inc. (CSXT) filed with the Surface Transportation Board a petition... must refer to Docket No. AB 55 (Sub-No. 727X) and must be sent to: (1) Surface Transportation...

  14. An endophytic taxol-producing fungus from Taxus media, Cladosporium cladosporioides MD2.

    PubMed

    Zhang, Peng; Zhou, Peng-Peng; Yu, Long-Jiang

    2009-09-01

    Fermentation processes using taxol-producing fungi other than Taxus spp. may be an alternative way to produce taxol, which is an important antitumor agent used widely in the clinic setting. In this study, a taxol-producing endophytic fungus strain MD2 was isolated from the inner bark of Taxus media. Strain MD2 produced taxol when grown in potato dextrose liquid medium. The fungal taxol-which was analyzed by ultraviolet, high-performance liquid chromatography and mass spectrometry-was shown to be identical to authentic taxol and 10-deacetylbaccatin III. Further analysis with nuclear magnetic resonance (NMR) spectroscopy to show the chemical structure of the fungal taxol indicated that the fungal taxol produced an NMR spectrum identical to that of authentic taxol. Strain MD2 was identified as Cladosporium cladosporioides according to morphology of the fungal culture, characteristics of the spores, and analysis of 18S rDNA sequence. In addition, 10-deacetylbaccatin III-10-O-acetyl transferase gene of C. cladosporioides MD2 was cloned for the first time and was shown to share 99% identity with that of T. x media and 97% identity with that of T. wallichiana var. mairei.

  15. 75 FR 26993 - Alvin Darby, M.D.; Denial of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... factors are * * * considered in the disjunctive.'' Robert A. Leslie, M.D., 68 FR 15227, 15230 (2003). I... application under section 303. See The Lawsons, Inc., 72 FR 74334, 74337 (2007); Anthony D. Funches, 64 FR 14267, 14268 (1999); Alan R. Schankman, 63 FR 45260 (1998); Kuen H. Chen, 58 FR 65401, 65402...

  16. 76 FR 53961 - Harold Edward Smith, M.D.; Revocation Of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ... * * * are considered in the disjunctive.'' Robert A. Leslie, 68 FR 15227, 15230 (2003). I may rely on any... interpretation of the law.'' Id. at 772 (int. quotations and citations omitted). See also Craig H. Bammer, 73 FR...\\ See Kenneth Wayne Green, Jr., M.D., 59 FR 51453, 51454 (1994) (registrant's ``continued drug usage...

  17. Validity of the Medical College Admission Test for predicting MD-PhD student outcomes.

    PubMed

    Bills, James L; VanHouten, Jacob; Grundy, Michelle M; Chalkley, Roger; Dermody, Terence S

    2016-03-01

    The Medical College Admission Test (MCAT) is a quantitative metric used by MD and MD-PhD programs to evaluate applicants for admission. This study assessed the validity of the MCAT in predicting training performance measures and career outcomes for MD-PhD students at a single institution. The study population consisted of 153 graduates of the Vanderbilt Medical Scientist Training Program (combined MD-PhD program) who matriculated between 1963 and 2003 and completed dual-degree training. This population was divided into three cohorts corresponding to the version of the MCAT taken at the time of application. Multivariable regression (logistic for binary outcomes and linear for continuous outcomes) was used to analyze factors associated with outcome measures. The MCAT score and undergraduate GPA (uGPA) were treated as independent variables; medical and graduate school grades, time-to-PhD defense, USMLE scores, publication number, and career outcome were dependent variables. For cohort 1 (1963-1977), MCAT score was not associated with any assessed outcome, although uGPA was associated with medical school preclinical GPA and graduate school GPA (gsGPA). For cohort 2 (1978-1991), MCAT score was associated with USMLE Step II score and inversely correlated with publication number, and uGPA was associated with preclinical GPA (mspGPA) and clinical GPA (mscGPA). For cohort 3 (1992-2003), the MCAT score was associated with mscGPA, and uGPA was associated with gsGPA. Overall, MCAT score and uGPA were inconsistent or weak predictors of training metrics and career outcomes for this population of MD-PhD students.

  18. MuSTAR MD: multi-scale sampling using temperature accelerated and replica exchange molecular dynamics.

    PubMed

    Yamamori, Yu; Kitao, Akio

    2013-10-14

    A new and efficient conformational sampling method, MuSTAR MD (Multi-scale Sampling using Temperature Accelerated and Replica exchange Molecular Dynamics), is proposed to calculate the free energy landscape on a space spanned by a set of collective variables. This method is an extension of temperature accelerated molecular dynamics and can also be considered as a variation of replica-exchange umbrella sampling. In the MuSTAR MD, each replica contains an all-atom fine-grained model, at least one coarse-grained model, and a model defined by the collective variables that interacts with the other models in the same replica through coupling energy terms. The coarse-grained model is introduced to drive efficient sampling of large conformational space and the fine-grained model can serve to conduct more accurate conformational sampling. The collective variable model serves not only to mediate the coarse- and fine-grained models, but also to enhance sampling efficiency by temperature acceleration. We have applied this method to Ala-dipeptide and examined the sampling efficiency of MuSTAR MD in the free energy landscape calculation compared to that for replica exchange molecular dynamics, replica exchange umbrella sampling, temperature accelerated molecular dynamics, and conventional MD. The results clearly indicate the advantage of sampling a relatively high energy conformational space, which is not sufficiently sampled with other methods. This feature is important in the investigation of transition pathways that go across energy barriers. MuSTAR MD was also applied to Met-enkephalin as a test case in which two Gō-like models were employed as the coarse-grained model.

  19. 78 FR 52109 - Proposed Amendment of Class D and E Airspace, and Establishment of Class E Airspace; Salisbury, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-22

    ... Wicomico Regional Airport, Salisbury, MD. Class D airspace and Class E surface area airspace would be... an extension to a Class D surface area airspace would be established within a 4.3-mile radius of the... Directory. Paragraph 6002 Class E airspace designated as surface areas. * * * * * AEA MD E2 Salisbury,...

  20. 76 FR 53442 - Viridian Energy MD LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Viridian Energy MD LLC; Supplemental Notice That Initial Market- Based Rate...-referenced proceeding of Viridian Energy MD LLC's application for market-based rate authority, with...

  1. The Convergence of Business and Medicine: A Study of MD/MBA Programs in the United States

    ERIC Educational Resources Information Center

    Keogh, Timothy J.; Martin, William Marty

    2011-01-01

    The purpose of this paper is to identify the convergence of business and medical education and describe the curricula of MD/MBA (Medical Doctor/Master of Business Administration) programs in the US. The focus of this study is to provide a guide to dual MD/MBA programs for physicians, aspiring physicians, policy makers and healthcare organizations.…

  2. Draft Genome Sequence of Ralstonia sp. MD27, a Poly(3-Hydroxybutyrate)-Degrading Bacterium, Isolated from Compost

    PubMed Central

    Zhu, Morgan; McCully, Lucy M.; Silby, Mark W.; Charles-Ogan, Tamunonengiyeofori I.

    2015-01-01

    Ralstonia sp. strain MD27, a novel biopolymer-degrading betaproteobacterium, was isolated from compost samples. This organism has been shown to utilize the biopolymer poly(3-hydroxybutyrate) [P(3HB)] as a carbon source for growth. We report the draft genome sequence of MD27 with an estimated total sequence length of 5.9 Mb. PMID:26450738

  3. 33 CFR 334.155 - Severn River, Naval Station Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area. 334.155 Section 334.155 Navigation and... RESTRICTED AREA REGULATIONS § 334.155 Severn River, Naval Station Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area. (a) The area. The waters within the Naval Station Annapolis small boat basin...

  4. 33 CFR 334.155 - Severn River, Naval Station Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area. 334.155 Section 334.155 Navigation and... RESTRICTED AREA REGULATIONS § 334.155 Severn River, Naval Station Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area. (a) The area. The waters within the Naval Station Annapolis small boat basin...

  5. 33 CFR 334.155 - Severn River, Naval Station Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area. 334.155 Section 334.155 Navigation and... RESTRICTED AREA REGULATIONS § 334.155 Severn River, Naval Station Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area. (a) The area. The waters within the Naval Station Annapolis small boat basin...

  6. 33 CFR 334.155 - Severn River, Naval Station Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area. 334.155 Section 334.155 Navigation and... RESTRICTED AREA REGULATIONS § 334.155 Severn River, Naval Station Annapolis, Small Boat Basin, Annapolis, MD; naval restricted area. (a) The area. The waters within the Naval Station Annapolis small boat basin...

  7. 33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and... Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and navigation. (a... regulations and monitor traffic through the canal. (c) Safe navigation required. Clearance for any vessel...

  8. Phagocytosis and intracellular killing of MD-2 opsonized Gram-negative bacteria depend on TLR4 signaling

    PubMed Central

    Jain, Vishal; Halle, Annett; Halmen, Kristen A.; Lien, Egil; Charrel-Dennis, Marie; Ram, Sanjay; Golenbock, Douglas T.

    2008-01-01

    Both Toll-like receptor 4 (TLR4)– and MD-2–deficient mice succumb to otherwise nonfatal Gram-negative bacteria inocula, demonstrating the pivotal role played by these proteins in antibacterial defense in mammals. MD-2 is a soluble endogenous ligand for TLR4 and a receptor for lipopolysaccharide (LPS). LPS-bound MD-2 transmits an activating signal onto TLR4. In this report, we show that both recombinant and endogenous soluble MD-2 bind tightly to the surface of live Gram-negative bacteria. As a consequence, MD-2 enhances cellular activation, bacterial internalization, and intracellular killing, all in a TLR4-dependent manner. The enhanced internalization of MD-2–coated bacteria was not observed in macrophages expressing Lpsd, a signaling-incompetent mutant form of TLR4, suggesting that the enhanced phagocytosis observed is dependent on signal transduction. The data confirm the notion that soluble MD-2 is a genuine opsonin that enhances proinflammatory opsonophagocytosis by bridging live Gram-negative bacteria to the LPS transducing complex. The presented results extend our understanding of the role of the TLR4/MD-2 signaling axis in bacterial recognition by phagocytes. PMID:18203953

  9. 33 CFR 334.170 - Chesapeake Bay, in the vicinity of Chesapeake Beach, Md.; firing range, Naval Research Laboratory.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... of Chesapeake Beach, Md.; firing range, Naval Research Laboratory. 334.170 Section 334.170 Navigation... RESTRICTED AREA REGULATIONS § 334.170 Chesapeake Bay, in the vicinity of Chesapeake Beach, Md.; firing range...-west line through Chesapeake Beach Light 2 at the entrance channel to Fishing Creek; on the south by...

  10. 33 CFR 334.170 - Chesapeake Bay, in the vicinity of Chesapeake Beach, Md.; firing range, Naval Research Laboratory.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... of Chesapeake Beach, Md.; firing range, Naval Research Laboratory. 334.170 Section 334.170 Navigation... RESTRICTED AREA REGULATIONS § 334.170 Chesapeake Bay, in the vicinity of Chesapeake Beach, Md.; firing range...-west line through Chesapeake Beach Light 2 at the entrance channel to Fishing Creek; on the south by...

  11. 33 CFR 334.170 - Chesapeake Bay, in the vicinity of Chesapeake Beach, Md.; firing range, Naval Research Laboratory.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of Chesapeake Beach, Md.; firing range, Naval Research Laboratory. 334.170 Section 334.170 Navigation... RESTRICTED AREA REGULATIONS § 334.170 Chesapeake Bay, in the vicinity of Chesapeake Beach, Md.; firing range...-west line through Chesapeake Beach Light 2 at the entrance channel to Fishing Creek; on the south by...

  12. 33 CFR 334.170 - Chesapeake Bay, in the vicinity of Chesapeake Beach, Md.; firing range, Naval Research Laboratory.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of Chesapeake Beach, Md.; firing range, Naval Research Laboratory. 334.170 Section 334.170 Navigation... RESTRICTED AREA REGULATIONS § 334.170 Chesapeake Bay, in the vicinity of Chesapeake Beach, Md.; firing range...-west line through Chesapeake Beach Light 2 at the entrance channel to Fishing Creek; on the south by...

  13. 33 CFR 165.556 - Regulated Navigation Area; Chesapeake and Delaware Canal, Chesapeake City Anchorage Basin, MD.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... areas, found in 33 CFR 165.13, apply to the regulated navigation area described in paragraph (a) of this...; Chesapeake and Delaware Canal, Chesapeake City Anchorage Basin, MD. 165.556 Section 165.556 Navigation and..., Chesapeake City Anchorage Basin, MD. (a) Location. The following area is a regulated navigation area:...

  14. 33 CFR 334.160 - Severn River, at U.S. Naval Academy Santee Basin, Annapolis, Md.; naval restricted area.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Academy Santee Basin, Annapolis, Md.; naval restricted area. 334.160 Section 334.160 Navigation and... RESTRICTED AREA REGULATIONS § 334.160 Severn River, at U.S. Naval Academy Santee Basin, Annapolis, Md.; naval restricted area. (a) The area. The waters within the U.S. Naval Academy Santee Basin and adjacent waters...

  15. Pathogenesis and protective efficacy of attenuated Meq null rMd5 virus in maternal antibody negative and commercial chickens.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A serotype 1 Marek’s disease Meq null virus (rMd5'Meq) has been shown to be an excellent vaccine in maternal antibody positive (MAb+) chickens. The only drawback of this non-oncogenic virus is that, like parental rMd5, it retains the ability to induce bursa and thymus atrophy (BTA) in MAb- chickens....

  16. Characterizing a point mutation within the UL5 gene that reduces in vivo replication and MD incidence

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Marek’s disease virus (MDV) is an oncogenic alphaherpesvirus of chickens and the causative agent for Marek’s disease (MD). Vaccination is the primary method to control MD. Despite the success of vaccination, more virulent field strains are evolving, requiring the development of new vaccines. Attenua...

  17. Measuring the effects of Meniere's disease: results of the Patient-Oriented Severity Index (MD POSI) version 1.

    PubMed

    Murphy, M P; Gates, G A

    1999-04-01

    We describe the development and initial psychometric testing of a new self-report instrument for quantifying the impact of Meniere's disease (MD) on patients' lives: the Meniere's Disease Patient-Oriented Severity Index (MD POSI). Eighty-five volunteers with chronic MD took a self-administered MD POSI. The internal consistency form of reliability was determined by calculating Cronbach's alpha. The relationship between total MD POSI problem severity scores and responses to a global question concerning the degree to which his or her MD had changed the patient's life assessed construct-related validity. The total problem severity score yielded excellent reliability (Cronbach's alpha = .928). No significant age or gender effects were seen. A highly statistically significant association was noted between total problem severity scores and the degree to which the patient's life had been changed by his or her MD (Spearman rank correlation = .526, p < .0001), indicating excellent construct-related validity. The material included in the initial questionnaire provides important information that can be used clinically to quantify the impact of MD on patients' health status. A beta version of the instrument is in the process of validation and determination of its sensitivity to change in health status.

  18. Assessing the allelotypic effect of two aminocyclopropane carboxylic acid synthase-encoding genes MdACS1 and MdACS3a on fruit ethylene production and softening in Malus.

    PubMed

    Dougherty, Laura; Zhu, Yuandi; Xu, Kenong

    2016-01-01

    Phytohormone ethylene largely determines apple fruit shelf life and storability. Previous studies demonstrated that MdACS1 and MdACS3a, which encode 1-aminocyclopropane-1-carboxylic acid synthases (ACS), are crucial in apple fruit ethylene production. MdACS1 is well-known to be intimately involved in the climacteric ethylene burst in fruit ripening, while MdACS3a has been regarded a main regulator for ethylene production transition from system 1 (during fruit development) to system 2 (during fruit ripening). However, MdACS3a was also shown to have limited roles in initiating the ripening process lately. To better assess their roles, fruit ethylene production and softening were evaluated at five time points during a 20-day post-harvest period in 97 Malus accessions and in 34 progeny from 2 controlled crosses. Allelotyping was accomplished using an existing marker (ACS1) for MdACS1 and two markers (CAPS866 and CAPS870) developed here to specifically detect the two null alleles (ACS3a-G289V and Mdacs3a) of MdACS3a. In total, 952 Malus accessions were allelotyped with the three markers. The major findings included: The effect of MdACS1 was significant on fruit ethylene production and softening while that of MdACS3a was less detectable; allele MdACS1-2 was significantly associated with low ethylene and slow softening; under the same background of the MdACS1 allelotypes, null allele Mdacs3a (not ACS3a-G289V) could confer a significant delay of ethylene peak; alleles MdACS1-2 and Mdacs3a (excluding ACS3a-G289V) were highly enriched in M. domestica and M. hybrid when compared with those in M. sieversii. These findings are of practical implications in developing apples of low and delayed ethylene profiles by utilizing the beneficial alleles MdACS1-2 and Mdacs3a. PMID:27231553

  19. Assessing the allelotypic effect of two aminocyclopropane carboxylic acid synthase-encoding genes MdACS1 and MdACS3a on fruit ethylene production and softening in Malus

    PubMed Central

    Dougherty, Laura; Zhu, Yuandi; Xu, Kenong

    2016-01-01

    Phytohormone ethylene largely determines apple fruit shelf life and storability. Previous studies demonstrated that MdACS1 and MdACS3a, which encode 1-aminocyclopropane-1-carboxylic acid synthases (ACS), are crucial in apple fruit ethylene production. MdACS1 is well-known to be intimately involved in the climacteric ethylene burst in fruit ripening, while MdACS3a has been regarded a main regulator for ethylene production transition from system 1 (during fruit development) to system 2 (during fruit ripening). However, MdACS3a was also shown to have limited roles in initiating the ripening process lately. To better assess their roles, fruit ethylene production and softening were evaluated at five time points during a 20-day post-harvest period in 97 Malus accessions and in 34 progeny from 2 controlled crosses. Allelotyping was accomplished using an existing marker (ACS1) for MdACS1 and two markers (CAPS866 and CAPS870) developed here to specifically detect the two null alleles (ACS3a-G289V and Mdacs3a) of MdACS3a. In total, 952 Malus accessions were allelotyped with the three markers. The major findings included: The effect of MdACS1 was significant on fruit ethylene production and softening while that of MdACS3a was less detectable; allele MdACS1–2 was significantly associated with low ethylene and slow softening; under the same background of the MdACS1 allelotypes, null allele Mdacs3a (not ACS3a-G289V) could confer a significant delay of ethylene peak; alleles MdACS1–2 and Mdacs3a (excluding ACS3a-G289V) were highly enriched in M. domestica and M. hybrid when compared with those in M. sieversii. These findings are of practical implications in developing apples of low and delayed ethylene profiles by utilizing the beneficial alleles MdACS1-2 and Mdacs3a. PMID:27231553

  20. MdMYB1 Regulates Anthocyanin and Malate Accumulation by Directly Facilitating Their Transport into Vacuoles in Apples1[OPEN

    PubMed Central

    Hu, Da-Gang; Sun, Cui-Hui; Ma, Qi-Jun; You, Chun-Xiang; Hao, Yu-Jin

    2016-01-01

    Tonoplast transporters, including proton pumps and secondary transporters, are essential for plant cell function and for quality formation of fleshy fruits and ornamentals. Vacuolar transport of anthocyanins, malate, and other metabolites is directly or indirectly dependent on the H+-pumping activities of vacuolar H+-ATPase (VHA) and/or vacuolar H+-pyrophosphatase, but how these proton pumps are regulated in modulating vacuolar transport is largely unknown. Here, we report a transcription factor, MdMYB1, in apples that binds to the promoters of two genes encoding the B subunits of VHA, MdVHA-B1 and MdVHA-B2, to transcriptionally activate its expression, thereby enhancing VHA activity. A series of transgenic analyses in apples demonstrates that MdMYB1/10 controls cell pH and anthocyanin accumulation partially by regulating MdVHA-B1 and MdVHA-B2. Furthermore, several other direct target genes of MdMYB10 are identified, including MdVHA-E2, MdVHP1, MdMATE-LIKE1, and MdtDT, which are involved in H+-pumping or in the transport of anthocyanins and malates into vacuoles. Finally, we show that the mechanism by which MYB controls malate and anthocyanin accumulation in apples also operates in Arabidopsis (Arabidopsis thaliana). These findings provide novel insights into how MYB transcription factors directly modulate the vacuolar transport system in addition to anthocyanin biosynthesis, consequently controlling organ coloration and cell pH in plants. PMID:26637549

  1. MdMYB1 Regulates Anthocyanin and Malate Accumulation by Directly Facilitating Their Transport into Vacuoles in Apples.

    PubMed

    Hu, Da-Gang; Sun, Cui-Hui; Ma, Qi-Jun; You, Chun-Xiang; Cheng, Lailiang; Hao, Yu-Jin

    2016-03-01

    Tonoplast transporters, including proton pumps and secondary transporters, are essential for plant cell function and for quality formation of fleshy fruits and ornamentals. Vacuolar transport of anthocyanins, malate, and other metabolites is directly or indirectly dependent on the H(+)-pumping activities of vacuolar H(+)-ATPase (VHA) and/or vacuolar H(+)-pyrophosphatase, but how these proton pumps are regulated in modulating vacuolar transport is largely unknown. Here, we report a transcription factor, MdMYB1, in apples that binds to the promoters of two genes encoding the B subunits of VHA, MdVHA-B1 and MdVHA-B2, to transcriptionally activate its expression, thereby enhancing VHA activity. A series of transgenic analyses in apples demonstrates that MdMYB1/10 controls cell pH and anthocyanin accumulation partially by regulating MdVHA-B1 and MdVHA-B2. Furthermore, several other direct target genes of MdMYB10 are identified, including MdVHA-E2, MdVHP1, MdMATE-LIKE1, and MdtDT, which are involved in H(+)-pumping or in the transport of anthocyanins and malates into vacuoles. Finally, we show that the mechanism by which MYB controls malate and anthocyanin accumulation in apples also operates in Arabidopsis (Arabidopsis thaliana). These findings provide novel insights into how MYB transcription factors directly modulate the vacuolar transport system in addition to anthocyanin biosynthesis, consequently controlling organ coloration and cell pH in plants. PMID:26637549

  2. Species-specific activation of TLR4 by hypoacylated endotoxins governed by residues 82 and 122 of MD-2.

    PubMed

    Oblak, Alja; Jerala, Roman

    2014-01-01

    The Toll-like receptor 4/MD-2 receptor complex recognizes endotoxin, a Gram-negative bacterial cell envelope component. Recognition of the most potent hexaacylated form of endotoxin is mediated by the sixth acyl chain that protrudes from the MD-2 hydrophobic pocket and bridges TLR4/MD-2 to the neighboring TLR4 ectodomain, driving receptor dimerization via hydrophobic interactions. In hypoacylated endotoxins all acyl chains could be accommodated within the binding pocket of the human hMD-2. Nevertheless, tetra- and pentaacylated endotoxins activate the TLR4/MD-2 receptor of several species. We observed that amino acid residues 82 and 122, located at the entrance to the endotoxin binding site of MD-2, have major influence on the species-specific endotoxin recognition. We show that substitution of hMD-2 residue V82 with an amino acid residue with a bulkier hydrophobic side chain enables activation of TLR4/MD-2 by pentaacylated and tetraacylated endotoxins. Interaction of the lipid A phosphate group with the amino acid residue 122 of MD-2 facilitates the appropriate positioning of the hypoacylated endotoxin. Moreover, mouse TLR4 contributes to the agonistic effect of pentaacylated msbB endotoxin. We propose a molecular model that explains how the molecular differences between the murine or equine MD-2, which both have sufficiently large hydrophobic pockets to accommodate all five or four acyl chains, influence the positioning of endotoxin so that one of the acyl chains remains outside the pocket and enables hydrophobic interactions with TLR4, leading to receptor activation.

  3. Moving Beyond Active-Site Detection: MixMD Applied to Allosteric Systems.

    PubMed

    Ghanakota, Phani; Carlson, Heather A

    2016-08-25

    Mixed-solvent molecular dynamics (MixMD) is a hotspot-mapping technique that relies on molecular dynamics simulations of proteins in binary solvent mixtures. Previous work on MixMD has established the technique's effectiveness in capturing binding sites of small organic compounds. In this work, we show that MixMD can identify both competitive and allosteric sites on proteins. The MixMD approach embraces full protein flexibility and allows competition between solvent probes and water. Sites preferentially mapped by probe molecules are more likely to be binding hotspots. There are two important requirements for the identification of ligand-binding hotspots: (1) hotspots must be mapped at very high signal-to-noise ratio and (2) the hotspots must be mapped by multiple probe types. We have developed our mapping protocol around acetonitrile, isopropanol, and pyrimidine as probe solvents because they allowed us to capture hydrophilic, hydrophobic, hydrogen-bonding, and aromatic interactions. Charged probes were needed for mapping one target, and we introduce them in this work. In order to demonstrate the robust nature and wide applicability of the technique, a combined total of 5 μs of MixMD was applied across several protein targets known to exhibit allosteric modulation. Most notably, all the protein crystal structures used to initiate our simulations had no allosteric ligands bound, so there was no preorganization of the sites to predispose the simulations to find the allosteric hotspots. The protein test cases were ABL Kinase, Androgen Receptor, CHK1 Kinase, Glucokinase, PDK1 Kinase, Farnesyl Pyrophosphate Synthase, and Protein-Tyrosine Phosphatase 1B. The success of the technique is demonstrated by the fact that the top-four sites solely map the competitive and allosteric sites. Lower-ranked sites consistently map other biologically relevant sites, multimerization interfaces, or crystal-packing interfaces. Lastly, we highlight the importance of including protein

  4. PoopMD, a Mobile Health Application, Accurately Identifies Infant Acholic Stools

    PubMed Central

    Franciscovich, Amy; Vaidya, Dhananjay; Doyle, Joe; Bolinger, Josh; Capdevila, Montserrat; Rice, Marcus; Hancock, Leslie; Mahr, Tanya; Mogul, Douglas B.

    2015-01-01

    Biliary atresia (BA) is the leading cause of pediatric end-stage liver disease in the United States. Education of parents in the perinatal period with stool cards depicting acholic and normal stools has been associated with improved time-to-diagnosis and survival in BA. PoopMD is a mobile application that utilizes a smartphone’s camera and color recognition software to analyze an infant’s stool and determine if additional follow-up is indicated. PoopMD was developed using custom HTML5/CSS3 and wrapped to work on iOS and Android platforms. In order to define the gold standard regarding stool color, seven pediatricians were asked to review 45 photographs of infant stool and rate them as acholic, normal, or indeterminate. Samples for which 6+ pediatricians demonstrated agreement defined the gold standard, and only these samples were included in the analysis. Accuracy of PoopMD was assessed using an iPhone 5s with incandescent lighting. Variability in analysis of stool photographs as acholic versus normal with intermediate rating weighted as 50% agreement (kappa) was compared between three laypeople and one expert user. Variability in output was also assessed between an iPhone 5s and a Samsung Galaxy S4, as well as between incandescent lighting and compact fluorescent lighting. Six-plus pediatricians agreed on 27 normal and 7 acholic photographs; no photographs were defined as indeterminate. The sensitivity was 7/7 (100%). The specificity was 24/27 (89%) with 3/27 labeled as indeterminate; no photos of normal stool were labeled as acholic. The Laplace-smoothed positive likelihood ratio was 6.44 (95% CI 2.52 to 16.48) and the negative likelihood ratio was 0.13 (95% CI 0.02 to 0.83). kappauser was 0.68, kappaphone was 0.88, and kappalight was 0.81. Therefore, in this pilot study, PoopMD accurately differentiates acholic from normal color with substantial agreement across users, and almost perfect agreement across two popular smartphones and ambient light settings

  5. PoopMD, a Mobile Health Application, Accurately Identifies Infant Acholic Stools.

    PubMed

    Franciscovich, Amy; Vaidya, Dhananjay; Doyle, Joe; Bolinger, Josh; Capdevila, Montserrat; Rice, Marcus; Hancock, Leslie; Mahr, Tanya; Mogul, Douglas B

    2015-01-01

    Biliary atresia (BA) is the leading cause of pediatric end-stage liver disease in the United States. Education of parents in the perinatal period with stool cards depicting acholic and normal stools has been associated with improved time-to-diagnosis and survival in BA. PoopMD is a mobile application that utilizes a smartphone's camera and color recognition software to analyze an infant's stool and determine if additional follow-up is indicated. PoopMD was developed using custom HTML5/CSS3 and wrapped to work on iOS and Android platforms. In order to define the gold standard regarding stool color, seven pediatricians were asked to review 45 photographs of infant stool and rate them as acholic, normal, or indeterminate. Samples for which 6+ pediatricians demonstrated agreement defined the gold standard, and only these samples were included in the analysis. Accuracy of PoopMD was assessed using an iPhone 5s with incandescent lighting. Variability in analysis of stool photographs as acholic versus normal with intermediate rating weighted as 50% agreement (kappa) was compared between three laypeople and one expert user. Variability in output was also assessed between an iPhone 5s and a Samsung Galaxy S4, as well as between incandescent lighting and compact fluorescent lighting. Six-plus pediatricians agreed on 27 normal and 7 acholic photographs; no photographs were defined as indeterminate. The sensitivity was 7/7 (100%). The specificity was 24/27 (89%) with 3/27 labeled as indeterminate; no photos of normal stool were labeled as acholic. The Laplace-smoothed positive likelihood ratio was 6.44 (95% CI 2.52 to 16.48) and the negative likelihood ratio was 0.13 (95% CI 0.02 to 0.83). kappauser was 0.68, kappaphone was 0.88, and kappalight was 0.81. Therefore, in this pilot study, PoopMD accurately differentiates acholic from normal color with substantial agreement across users, and almost perfect agreement across two popular smartphones and ambient light settings. PoopMD

  6. PoopMD, a Mobile Health Application, Accurately Identifies Infant Acholic Stools.

    PubMed

    Franciscovich, Amy; Vaidya, Dhananjay; Doyle, Joe; Bolinger, Josh; Capdevila, Montserrat; Rice, Marcus; Hancock, Leslie; Mahr, Tanya; Mogul, Douglas B

    2015-01-01

    Biliary atresia (BA) is the leading cause of pediatric end-stage liver disease in the United States. Education of parents in the perinatal period with stool cards depicting acholic and normal stools has been associated with improved time-to-diagnosis and survival in BA. PoopMD is a mobile application that utilizes a smartphone's camera and color recognition software to analyze an infant's stool and determine if additional follow-up is indicated. PoopMD was developed using custom HTML5/CSS3 and wrapped to work on iOS and Android platforms. In order to define the gold standard regarding stool color, seven pediatricians were asked to review 45 photographs of infant stool and rate them as acholic, normal, or indeterminate. Samples for which 6+ pediatricians demonstrated agreement defined the gold standard, and only these samples were included in the analysis. Accuracy of PoopMD was assessed using an iPhone 5s with incandescent lighting. Variability in analysis of stool photographs as acholic versus normal with intermediate rating weighted as 50% agreement (kappa) was compared between three laypeople and one expert user. Variability in output was also assessed between an iPhone 5s and a Samsung Galaxy S4, as well as between incandescent lighting and compact fluorescent lighting. Six-plus pediatricians agreed on 27 normal and 7 acholic photographs; no photographs were defined as indeterminate. The sensitivity was 7/7 (100%). The specificity was 24/27 (89%) with 3/27 labeled as indeterminate; no photos of normal stool were labeled as acholic. The Laplace-smoothed positive likelihood ratio was 6.44 (95% CI 2.52 to 16.48) and the negative likelihood ratio was 0.13 (95% CI 0.02 to 0.83). kappauser was 0.68, kappaphone was 0.88, and kappalight was 0.81. Therefore, in this pilot study, PoopMD accurately differentiates acholic from normal color with substantial agreement across users, and almost perfect agreement across two popular smartphones and ambient light settings. PoopMD

  7. Baltimore, MD

    NASA Technical Reports Server (NTRS)

    2001-01-01

    Baltimore is the largest city in Maryland and one of the busiest ports in the United States. Its economy focuses on research and development, especially in the areas of aquaculture, pharmaceuticals, and medical supplies and services. Before European settlement, the site of Baltimore was inhabited by Native Americans of the Susquehannock tribe. The town was founded in 1729 and named for the barons Baltimore, the British founders of the Maryland Colony. This ASTER image of Baltimore was acquired on April 4, 2000, covers an area of 17 by 20 km, and is located at 39.3 degrees north latitude and 76.7 degrees west longitude.

    The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission Directorate.

  8. A High-Resolution Carbonate Record from Holocene IMAGES core MD992286, Skagerrak

    NASA Astrophysics Data System (ADS)

    Gyllencreutz, R.

    2003-04-01

    Marine sediments in the 32.4 m long piston core MD99-2286, provides a continuous and detailed palaeoceanographic and palaeoenvironmenatal record of the last 12 000 years in the Skagerrak. The investigated area is characterised by high sedimentation rates and intense water mass mixing, as a branch of the North Atlantic Current turns anti-clockwise, slows down and becomes mixed with other waters to form the Norwegian Coastal Current. Present-day SSTs in Skagerrak are strongly linked to the NAO-index. Carbonate content in core MD99-2286 was measured using coulometry with 5-cm resolution. The carbonate record shows a steep rise from 9 to 15 % carbonate from 10 ka to 8 ka, and then a gradual decreasing trend down to about 12 % around 4 500 cal y BP. The timing of the peak at 8 ka coincides with the opening of the English Channel. The most likely processes controlling carbonate content in the Skagerrak sediments are redeposition of older carbonate particles (mainly controlled by the Jutland Current) and primary productivity competing with dilution by terrigenous material. The carbonate record from MD99-2286 correlates with a palaeotemperature reconstruction based on oxygen isotopes from a speleothem in northern Norway. The correlation is positive in the interval 9 ka to about 5 ka, and negative from 5 ka to present. The timing of the shift in correlation matches the establishment of the modern circulation pattern, which is marked by a hydrographic shift at 5.5 ka, interpreted from grain size and biostratigraphical data in a core from Skagen. This shift is manifested by an increase in the Jutland Current and stronger inflow of saline North Sea water to Skagerrak and Kattegat. The CALYPSO-corer used for core MD99-2286 is believed to disturb the topmost sediments in cores. Therefore, a 2.5 m long gravity core, Sk000209-2, was retrieved from a nearby location in order to get full recovery of the surface sediments. Age control in core MD99-2286 is obtained from 20 AMS C-14

  9. MD-CTS: An integrated terminology reference of clinical and translational medicine

    PubMed Central

    Ray, Will; Finamore, Joe; Rastegar-Mojarad, Majid; Kadolph, Chris; Ye, Zhan; Bohne, Jacquie; Xu, Yin; Burish, Dan; Sondelski, Joshua; Easker, Melissa; Finnegan, Brian; Bartkowiak, Barbara; Smith, Catherine Arnott; Tachinardi, Umberto; Mendonca, Eneida A.; Weichelt, Bryan; Lin, Simon M.

    2016-01-01

    New vocabularies are rapidly evolving in the literature relative to the practice of clinical medicine and translational research. To provide integrated access to new terms, we developed a mobile and desktop online reference—Marshfield Dictionary of Clinical and Translational Science (MD-CTS). It is the first public resource that comprehensively integrates Wiktionary (word definition), BioPortal (ontology), Wiki (image reference), and Medline abstract (word usage) information. MD-CTS is accessible at http://spellchecker.mfldclin.edu/. The website provides a broadened capacity for the wider clinical and translational science community to keep pace with newly emerging scientific vocabulary. An initial evaluation using 63 randomly selected biomedical words suggests that online references generally provided better coverage (73%-95%) than paper-based dictionaries (57–71%). PMID:27069559

  10. Structure of Anion-Conducting Polymers from Waxs and MD Simulations

    NASA Astrophysics Data System (ADS)

    Frisken, Barbara; Tahmasebi, Sepehr; Schibli, Eric; Holdcroft, Steven

    The structure of novel polymers for anion exchange membranes (AEMs) is investigated using wide angle X-ray scattering (WAXS) combined with molecular dynamics (MD) simulations using a united-atom force field model based on the DREIDING force field. The polymers being studied are poly(benzimidazole) (PBI) derivatives including poly(dimethylbenzimidazole) (PDMBI), mesitylene poly(benzimidazole) (mes-PBI), and mesitylene poly(dimethylbenzimidazole) (mes-PDMBI). WAXS reveals an amorphous structure with two main length scales. By comparing simulation results to WAXS data, we attribute features observed in the scattering data to side-to-side spacing between polymer chains and to the parallel-ring stacking of the benzimidazole rings. Overall, we are able to validate the interpretation of scattering data by combining MD simulations and scattering experiments.

  11. Mimicking titration experiments with MD simulations: A protocol for the investigation of pH-dependent effects on proteins

    NASA Astrophysics Data System (ADS)

    Socher, Eileen; Sticht, Heinrich

    2016-03-01

    Protein structure and function are highly dependent on the environmental pH. However, the temporal or spatial resolution of experimental approaches hampers direct observation of pH-induced conformational changes at the atomic level. Molecular dynamics (MD) simulation strategies (e.g. constant pH MD) have been developed to bridge this gap. However, one frequent problem is the sampling of unrealistic conformations, which may also lead to poor pKa predictions. To address this problem, we have developed and benchmarked the pH-titration MD (pHtMD) approach, which is inspired by wet-lab titration experiments. We give several examples how the pHtMD protocol can be applied for pKa calculation including peptide systems, Staphylococcus nuclease (SNase), and the chaperone HdeA. For HdeA, pHtMD is also capable of monitoring pH-dependent dimer dissociation in accordance with experiments. We conclude that pHtMD represents a versatile tool for pKa value calculation and simulation of pH-dependent effects in proteins.

  12. Mimicking titration experiments with MD simulations: A protocol for the investigation of pH-dependent effects on proteins.

    PubMed

    Socher, Eileen; Sticht, Heinrich

    2016-03-03

    Protein structure and function are highly dependent on the environmental pH. However, the temporal or spatial resolution of experimental approaches hampers direct observation of pH-induced conformational changes at the atomic level. Molecular dynamics (MD) simulation strategies (e.g. constant pH MD) have been developed to bridge this gap. However, one frequent problem is the sampling of unrealistic conformations, which may also lead to poor pKa predictions. To address this problem, we have developed and benchmarked the pH-titration MD (pHtMD) approach, which is inspired by wet-lab titration experiments. We give several examples how the pHtMD protocol can be applied for pKa calculation including peptide systems, Staphylococcus nuclease (SNase), and the chaperone HdeA. For HdeA, pHtMD is also capable of monitoring pH-dependent dimer dissociation in accordance with experiments. We conclude that pHtMD represents a versatile tool for pKa value calculation and simulation of pH-dependent effects in proteins.

  13. 76 FR 17672 - Alfred E. Boyce, M.D.; Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-30

    ... v. U.S. Dept. of Justice, 873 F.2d 1089, 1091 (8th Cir. 1989); Thomas E. Johnston, 45 FR 72311 (1980... '' (emphasis supplied). Roy Chi Lung, 74 FR 20346, 20347 (2009); Scott Sandarg, D.M.D., 74 FR 17528, 174529 (2009); John B. Freitas, D.O., 74 FR 17524, 17525 (2009); Roger A. Rodriguez, M.D., 70 FR 33206,...

  14. Unusually low fragment energies in the symmetric fission of /sup 259/Md

    SciTech Connect

    Wild, J.F.; Hulet, E.K.; Lougheed, R.W.; Baisden, P.A.; Landrum, J.H.; Dougan, R.J.; Mustafa, M.G.

    1982-10-01

    The 103-min isotope /sup 259/Md has been identified as the daughter of an electron-capture decay branch of /sup 259/No produced via the /sup 248/Cm(/sup 18/O,..cap alpha..3n) reaction. Chemical separations were used to confirm the identity of /sup 259/Md, which decays by spontaneous fission. The kinetic energies of coincident fission fragments were measured, corresponding to a fragment mass which is highly symmetric, similar to those of /sup 258/Fm and /sup 259/Fm. However, the total kinetic energy distribution for /sup 259/Md is considerably broader (FWHM approx.60 MeV) than those of /sup 258/Fm and /sup 259/Fm, and peaks at 201 MeV, about 35--40 MeV lower in energy. Furthermore, the maximum total Kinetic energy of 215 MeV for mass-symmetric events is about 30 MeV lower than for similar events from the spontaneous fission of /sup 258/Fm and /sup 259/Fm. A hypothesis that this energy difference resulted from the emission of light, hydrogen-like particles at scission in a large fraction of /sup 259/Md spontaneous fission decays was shown to be unfounded. From experiments to observe such particles with counter telescopes, an upper limit of 5% was determined for the fraction of fission events accompanied by light-particle emission. The total kinetic energy deficit at mass symmetry must, therefore, be distributed between internal excitation energy and fragment deformation energy at scission. Although the presence of a large amount of fragment deformation energy seems incompatible with symmetric fission into spherical Sn-like fragments, we prefer this explanation because the low total kinetic energy suggests a lowered Coulomb energy resulting from greater separation of the charge centers of deformed fragments at scission.

  15. Recognition of lipid A variants by the TLR4-MD-2 receptor complex

    PubMed Central

    Maeshima, Nina; Fernandez, Rachel C.

    2012-01-01

    Lipopolysaccharide (LPS) is a component of the outer membrane of almost all Gram-negative bacteria and consists of lipid A, core sugars, and O-antigen. LPS is recognized by Toll-like receptor 4 (TLR4) and MD-2 on host innate immune cells and can signal to activate the transcription factor NFκB, leading to the production of pro-inflammatory cytokines that initiate and shape the adaptive immune response. Most of what is known about how LPS is recognized by the TLR4-MD-2 receptor complex on animal cells has been studied using Escherichia coli lipid A, which is a strong agonist of TLR4 signaling. Recent work from several groups, including our own, has shown that several important pathogenic bacteria can modify their LPS or lipid A molecules in ways that significantly alter TLR4 signaling to NFκB. Thus, it has been hypothesized that expression of lipid A variants is one mechanism by which pathogens modulate or evade the host immune response. Additionally, several key differences in the amino acid sequences of human and mouse TLR4-MD-2 receptors have been shown to alter the ability to recognize these variations in lipid A, suggesting a host-specific effect on the immune response to these pathogens. In this review, we provide an overview of lipid A variants from several human pathogens, how the basic structure of lipid A is recognized by mouse and human TLR4-MD-2 receptor complexes, as well as how alteration of this pattern affects its recognition by TLR4 and impacts the downstream immune response. PMID:23408095

  16. The path ahead for MD/PhD programs in Canada Commentary on Jones et al.

    PubMed

    Rosenblum, Norman D

    2016-09-11

    The paper by Jones and colleagues, published in this edition of Clinical Investigative Medicine, contributes to our understanding of Canadian MD/PhD Programs. While there has been little published on this subject by the Canadian programs, themselves, this paper is the most recent in a series by leaders of the Clinical Investigator Trainee Association of Canada (CITAC). The authors are to be commended for their efforts and productivity.

  17. Hybrid MD-Nernst Planck Model of Alpha-hemolysin Conductance Properties

    NASA Technical Reports Server (NTRS)

    Cozmuta, Ioana; O'Keefer, James T.; Bose, Deepak; Stolc, Viktor

    2006-01-01

    Motivated by experiments in which an applied electric field translocates polynucleotides through an alpha-hemolysin protein channel causing ionic current transient blockade, a hybrid simulation model is proposed to predict the conductance properties of the open channel. Time scales corresponding to ion permeation processes are reached using the Poisson-Nemst-Planck (PNP) electro-diffusion model in which both solvent and local ion concentrations are represented as a continuum. The diffusion coefficients of the ions (K(+) and Cl(-)) input in the PNP model are, however, calculated from all-atom molecular dynamics (MD). In the MD simulations, a reduced representation of the channel is used. The channel is solvated in a 1 M KCI solution, and an external electric field is applied. The pore specific diffusion coefficients for both ionic species are reduced 5-7 times in comparison to bulk values. Significant statistical variations (17-45%) of the pore-ions diffusivities are observed. Within the statistics, the ionic diffusivities remain invariable for a range of external applied voltages between 30 and 240mV. In the 2D-PNP calculations, the pore stem is approximated by a smooth cylinder of radius approx. 9A with two constriction blocks where the radius is reduced to approx. 6A. The electrostatic potential includes the contribution from the atomistic charges. The MD-PNP model shows that the atomic charges are responsible for the rectifying behaviour and for the slight anion selectivity of the a-hemolysin pore. Independent of the hierarchy between the anion and cation diffusivities, the anionic contribution to the total ionic current will dominate. The predictions of the MD-PNP model are in good agreement with experimental data and give confidence in the present approach of bridging time scales by combining a microscopic and macroscopic model.

  18. The path ahead for MD/PhD programs in Canada Commentary on Jones et al.

    PubMed

    Rosenblum, Norman D

    2016-01-01

    The paper by Jones and colleagues, published in this edition of Clinical Investigative Medicine, contributes to our understanding of Canadian MD/PhD Programs. While there has been little published on this subject by the Canadian programs, themselves, this paper is the most recent in a series by leaders of the Clinical Investigator Trainee Association of Canada (CITAC). The authors are to be commended for their efforts and productivity. PMID:27619401

  19. Comparison of fission modes in {sup 252}Cf, {sup 257}Fm, and {sup 260}Md

    SciTech Connect

    Aarle, J. van; Siemon, K.; Patzelt, P.; Wild, J. F.; Lougheed, R. W.; Westmeier, W.

    1998-10-26

    Although the spontaneous-fission properties of heavy actinides have been studied for well over 35 years, many interesting and informative details continue to come into light. During the last decade, the spontaneous fission of {sup 252}Cf, {sup 257}Fm and {sup 260}Md has been extensively investigated at the Philipps University of Marburg, by means of a gadolinium-doped liquid scintillation tank for neutron counting and surface barrier detectors for fission fragment detection. The three nuclides represent the transition from the well-known asymmetric fission yield distribution, as it is characteristic for {sup 252}Cf, to a much more symmetrical one, found in the fission of {sup 260}Md. Therefore, trends in the dynamical changes of fission properties have been derived from these studies. For the spontaneous fission of {sup 252}Cf and {sup 260}Md, it was already shown that different fission modes, as proposed by theoretical calculations of Brosa et al. (5), could be separated, using the correlation between the neutrons emitted in a fission event and both the observed fission-fragment mass and the total kinetic energy (1, 2). In the case of {sup 257}Fm, no theoretical calculations for fission modes exist. However, from the fission properties of the two surrounding actinides, one can expect at least three different fission modes, namely two 'standard' and the 'supershort' mode. In this paper, results from the recent {sup 257}Fm experiment will be presented and compared to systematics extracted from the fission properties of other heavy actinides.

  20. mdRNA-Seq analysis of marine microbial communities from the northern Red Sea

    PubMed Central

    Hou, Shengwei; Pfreundt, Ulrike; Miller, Dan; Berman-Frank, Ilana; Hess, Wolfgang R.

    2016-01-01

    Metatranscriptomic differential RNA-Seq (mdRNA-Seq) identifies the suite of active transcriptional start sites at single-nucleotide resolution through enrichment of primary transcript 5′ ends. Here we analyzed the microbial community at 45 m depth at Station A in the northern Gulf of Aqaba, Red Sea, during 500 m deep mixing in February 2012 using mdRNA-Seq and a parallel classical RNA-Seq approach. We identified promoters active in situ for five different pico-planktonic genera (the SAR11 clade of Alphaproteobacteria, Synechococcus of Cyanobacteria, Euryarchaeota, Thaumarchaeota, and Micromonas as an example for picoeukaryotic algae), showing the applicability of this approach to highly diverse microbial communities. 16S rDNA quantification revealed that 24% of the analyzed community were group II marine Euryarchaeota in which we identified a highly abundant non-coding RNA, Tan1, and detected very high expression of genes encoding intrinsically disordered proteins, as well as enzymes for the synthesis of specific B vitamins, extracellular peptidases, carbohydrate-active enzymes, and transport systems. These results highlight previously unknown functions of Euryarchaeota with community-wide relevance. The complementation of metatranscriptomic studies with mdRNA-Seq provides substantial additional information regarding transcriptional start sites, promoter activities, and the identification of non-coding RNAs. PMID:27759035

  1. An Integrated Procedure for Tree N-body Simulations: FLY and AstroMD

    NASA Astrophysics Data System (ADS)

    Becciani, U.; Antonuccio-Delogu, V.; Buonomo, F.; Gheller, C.

    We present a new code for evolving three-dimensional self-gravitating collisionless systems with a large number of particles N >= 107. FLY (Fast Level-based N-bodY code) is a fully parallel code based on a tree algorithm. It adopts periodic boundary conditions implemented by means of the Ewald summation technique. FLY is based on the one-side communication paradigm for sharing data among the processors that access remote private data, avoiding any kind of synchronization. The code was originally developed on a CRAY T3E system using the SHMEM library and it was ported to SGI ORIGIN 2000 and IBM SP (on the latter making use of the LAPI library). FLY version 1.1 is open source, freely available code. FLY output data can be analysed with AstroMD, an analysis and visualization tool specifically designed for astrophysical data. AstroMD can manage different physical quantities. It can find structures without well defined shape or symmetries, and perform quantitative calculations on selected regions. AstroMD is freely available.

  2. Wettability of graphitic-carbon and silicon surfaces: MD modeling and theoretical analysis

    SciTech Connect

    Ramos-Alvarado, Bladimir; Kumar, Satish; Peterson, G. P.

    2015-07-28

    The wettability of graphitic carbon and silicon surfaces was numerically and theoretically investigated. A multi-response method has been developed for the analysis of conventional molecular dynamics (MD) simulations of droplets wettability. The contact angle and indicators of the quality of the computations are tracked as a function of the data sets analyzed over time. This method of analysis allows accurate calculations of the contact angle obtained from the MD simulations. Analytical models were also developed for the calculation of the work of adhesion using the mean-field theory, accounting for the interfacial entropy changes. A calibration method is proposed to provide better predictions of the respective contact angles under different solid-liquid interaction potentials. Estimations of the binding energy between a water monomer and graphite match those previously reported. In addition, a breakdown in the relationship between the binding energy and the contact angle was observed. The macroscopic contact angles obtained from the MD simulations were found to match those predicted by the mean-field model for graphite under different wettability conditions, as well as the contact angles of Si(100) and Si(111) surfaces. Finally, an assessment of the effect of the Lennard-Jones cutoff radius was conducted to provide guidelines for future comparisons between numerical simulations and analytical models of wettability.

  3. Cryptanalysis of Two MD5-Based Authentication Protocols: APOP and NMAC

    NASA Astrophysics Data System (ADS)

    Wang, Lei; Ohta, Kazuo; Sasaki, Yu; Sakiyama, Kazuo; Kunihiro, Noboru

    Many hash-based authentication protocols have been proposed, and proven secure assuming that underlying hash functions are secure. On the other hand, if a hash function compromises, the security of authentication protocols based on this hash function becomes unclear. Therefore, it is significantly important to verify the security of hash-based protocols when a hash function is broken. In this paper, we will re-evaluate the security of two MD5-based authentication protocols based on a fact that MD5 cannot satisfy a required fundamental property named collision resistance. The target protocols are APOP (Authenticated Post Office Protocol) and NMAC (Nested Message Authentication Code), since they or their variants are widely used in real world. For security evaluation of APOP, we will propose a modified password recovery attack procedure, which is twice as fast as previous attacks. Moreover, our attack is more realistic, as the probability of being detected is lower than that of previous attacks. For security evaluation of MD5-based NMAC, we will propose a new key-recovery attack procedure, which has a complexity lower than that of previous attack. The complexity of our attack is 276, while that of previous attack is 2100.**Moreover, our attack has another interesting point. NMAC has two keys: the inner key and the outer key. Our attack can recover the outer key partially without the knowledge of the inner key.

  4. Structural and Dynamic Analysis on IDPs by Modified AWSEM-MD

    NASA Astrophysics Data System (ADS)

    Wu, Hao; Papoian, Garegin; Papoian Theoretical Biophysics Group Team

    Unlike globular proteins, intrinsically disordered proteins (IDPs) lack both secondary and tertiary structures and can play key roles in various biological processes, including transcriptional regulation, molecular recognition and cellular signaling. These functions can be potentially elucidated by structural heterogeneity of IDPs. Because of their flexibility and disordered nature, it has been difficult to investigate IDPs both computationally and experimentally. In particular, it is desirable to develop coarse-grained, yet accurate models of IDPs, such that simulations exploring sufficient conformational ensembles could be carried out within feasible times. To achieve this goal, we modified the associative memory, water mediated, structure and energy model (AWSEM-MD), which is typically used for folding of globular proteins or binding studies. We tested modified AWSEM-MD on several well-studied IDPs and found the transient secondary structure propensity is consistent with NMR experimental results. The rugged free energy landscapes obtained also show structural heterogeneity of these IDPs. Our proposed extension of AWSEM-MD may allow simulating a wider range of IDPs with high accuracy and computational efficiency.

  5. Migration and interpolation for the hand-held GPR MD sensor system (ALIS)

    NASA Astrophysics Data System (ADS)

    Feng, Xuan; Kobayashi, Takao; Takahashi, Kazunori; Fujiwara, Jun; Sato, Motoyuki

    2006-05-01

    We developed a hand-held landmine detection sensor system, ALIS (Advanced Landmine Imaging System), combined with a metal detector and GPR (Ground penetrating radar). The system has a CCD camera attached on the sensor handle and can record the MD and GPR signal with the sensor position information. Therefore, it can offer the visual MD image and GPR image, which is used to define targets. But because ALIS is a hand-held system, the sensor position is random when it is operated in the field by human being. Also GPR normally suffers from very strong clutter. To deal with these problems, the interpolation is a common choice for both MD and GPR to create grid data set firstly and migration was used to improve the quality of GPR image. But generally the interpolation can not improve the quality of data set, although it can offer grid data set for visualization. Also for 3D GPR data set, it will consume much processing time. In fact, the migration can not only improve the quality of GPR data but also interpolate data to offer grid data set. It is a kind of 2.5D interpolation and just uses related data in the diffraction trajectory surface. So it can offer directly the visual GPR image and save the processing time. We will discuss two procedures for GPR, interpolation + migration or only migration, in this paper. Lastly, we also will report some results of evaluation test in 2006 February in Croatia.

  6. Solvation structure and dynamics of Na+ in liquid ammonia studied by ONIOM-XS MD simulations

    NASA Astrophysics Data System (ADS)

    Sripradite, Jarukorn; Tongraar, Anan; Kerdcharoen, Teerakiat

    2015-12-01

    The molecular dynamics (MD) technique based on the ONIOM-XS method, known as the ONIOM-XS MD, has been applied to investigate the solvation structure and dynamics of Na+ in liquid ammonia. Regarding the ONIOM-XS MD results, it is observed that Na+ is able to order the surrounding ammonia molecules to form its specific first and second solvation shells with the average coordination numbers of 5.1 and 11.2, respectively. The first solvation shell of Na+ is rather well-defined, forming a preferred 5-fold coordinated complex with a distorted square pyramidal geometry. In this respect, the most preferential Na+(NH3)5 species could convert back and forth to the lower probability Na+(NH3)6 and Na+(NH3)4 configurations. The second solvation shell of Na+ is detectable, in which a number of ammonia molecules, ranging from 7 to 14, are involved in this layer and they are arranged according to recognizable influence of the ion.

  7. Agrobacterium tumefaciens-mediated transformation of a taxol-producing endophytic fungus, Cladosporium cladosporioides MD2.

    PubMed

    Zhang, Peng; Liu, Ting-Ting; Zhou, Peng-Peng; Li, Shu-Tao; Yu, Long-Jiang

    2011-04-01

    In this study, an Agrobacteriurn tumefaciens-mediated transformation (ATMT) protocol was successfully developed for the genetic transformation of a taxol-producing fungus, Cladosporium cladosporioides MD2, and the co-cultivation conditions affecting the transformation efficiency were optimized. The optimal transformation conditions were that 1 ml of C. cladosporioides MD2 spore suspension (10(8) spores/ml) was mixed with an equal volume of A. tumefaciens cultures, which contained 400 μl of A. tumefaciens LBA4404 (OD(660) ≈ 0.6) and 600 μl LB medium that were used to make up difference in volume, and the mix cultures were supplemented with 300 μM acetosyringone (AS) and co-cultivated at 26°C and 50 rpm for 48 h. Stable transformants were obtained through analysis of the mitotic stability of inserted T-DNA and the presence of hygromycin resistance gene (hpt II). This study laid a fine groundwork for development of transgenic C. cladosporioides MD2 strains.

  8. Theoretical investigation of loratadine reactivity in order to understand its degradation properties: DFT and MD study.

    PubMed

    Armaković, Stevan; Armaković, Sanja J; Abramović, Biljana F

    2016-10-01

    Antihistamines are frequently used pharmaceuticals that treat the symptoms of allergic reactions. Loratadine (LOR) is an active component of the second generation of selective antihistaminic pharmaceutical usually known as Claritin. Frequent usage of this type of pharmaceuticals imposes the need for understanding their fundamental reactive properties. In this study we have theoretically investigated reactive properties of LOR using both density functional theory (DFT) calculations and molecular dynamics (MD) simulations. DFT study is used for collecting information related to the molecule stability, structure, frontier molecular orbitals, quantum molecular descriptors, charge distribution, molecular electrostatic potential surfaces, charge polarization, and local reactivity properties according to average local ionization energy surfaces. Based on these results, N24 atom of pyridine ring and oxygen atom O1 were identified with nucleophilic nature. In order to collect the information necessary for the proposition of degradation compounds we also calculated bond dissociation energies (BDE) for hydrogen abstraction and single acyclic bonds as well. According to BDE, the oxidation is likely to occur in the piperidine and cycloheptane rings. MD simulations were used in order to understand the interactions with water through radial distribution functions (RDF). Based on RDFs the most important interactions with solvent are determined for carbon atom C5, chlorine atom Cl15, and oxygen atom O1. Collected results based on DFT calculations and MD simulations provided information important for suggestion of possible degradation compounds. Covalent and noncovalent interactions between LOR and (•)OH have also been investigated. PMID:27640160

  9. The transcriptional response of apple alcohol acyltransferase (MdAAT2) to salicylic acid and ethylene is mediated through two apple MYB TFs in transgenic tobacco.

    PubMed

    Li, Peng-Cheng; Yu, Shao-Wei; Shen, Jin; Li, Qing-Qing; Li, Da-Peng; Li, De-Quan; Zheng, Cheng-Chao; Shu, Huai-Rui

    2014-08-01

    Volatile esters are major factors affecting the aroma of apple fruits, and alcohol acyltransferases (AATs) are key enzymes involved in the last steps of ester biosynthesis. The expression of apple AAT (MdAAT2) is known to be induced by salicylic acid (SA) or ethylene in apple fruits, although the mechanism of its transcriptional regulation remains elusive. In this study, we reveal that two apple transcription factors (TFs), MdMYB1 and MdMYB6, are involved in MdAAT2 promoter response to SA and ethylene in transgenic tobacco. According to electrophoretic mobility shift assays, MdMYB1 or MdMYB6 can directly bind in vitro to MYB binding sites in the MdAAT2 promoter. In vivo, overexpression of the two MYB TFs can greatly enhance MdAAT2 promoter activity, as demonstrated by dual luciferase reporter assays in transgenic tobacco. In contrast to the promoter of MdMYB1 or MdMYB6, the MdAAT2 promoter cannot be induced by SA or ethephon (ETH) in transgenic tobacco, even in stigmas in which the MdAAT2 promoter can be highly induced under normal conditions. However, the induced MYB TFs can dramatically enhance MdAAT2 promoter activity under SA or ETH treatment. We conclude that MdMYB1 and MdMYB6 function in MdAAT2 responses to SA and ethylene in transgenic tobacco, suggesting that a similar regulation mechanism may exist in apple.

  10. The topology of foliations formed by the generic K-orbits of a subclass of the indecomposable MD5-groups

    NASA Astrophysics Data System (ADS)

    Vu, Le Anh; Hoa, Duong Quang

    2009-03-01

    The present paper is a continuation of [13], [14] of the authors. Specifically, the paper considers the MD5-foliations associated to connected and simply connected MD5-groups such that their Lie algebras have 4-dimensional commutative derived ideal. In the paper, we give the topological classification of all considered MD5-foliations. A description of these foliations by certain fibrations or suitable actions of $\\mathbb{R}^{2}$ and the Connes' C*-algebras of the foliations which come from fibrations are also given in the paper.

  11. Spontaneous fission properties of sup 258 Fm, sup 259 Md, sup 260 Md, sup 258 No, and sup 260 (104): Bimodal fission

    SciTech Connect

    Hulet, E.K.; Wild, J.F.; Dougan, R.J.; Lougheed, R.W.; Landrum, J.H.; Dougan, A.D.; Baisden, P.A.; Henderson, C.M.; Dupzyk, R.J. ); Hahn, R.L.; and others

    1989-08-01

    We have measured the mass and kinetic-energy distributions from the spontaneous fission of {sup 258}Fm, {sup 258}No, {sup 259}Md, {sup 260}Md, and {sup 260}(104). All are observed to fission with a symmetrical division of mass. The total-kinetic-energy distributions strongly deviated from the Gaussian shape characteristically found in the fission of all other actinides. When the total-kinetic-energy distributions are resolved into two Gaussians, the constituent peaks lie near 200 and 233 MeV. We conclude that both low- and high-energy fission modes occur in four of the five nuclides studied. We call this property bimodal fission.'' Even though both modes are possible in the same nuclide, one generally predominates. We offer an explanation for each mode based on shell structures of the fissioning nucleus and of its fragments. The appearance of both modes of fission in this region of the nuclide chart seems to be a coincidence in that the opportunity to divide into near doubly magic Sn fragments occurs in the same region where the second fission barrier is expected to drop in energy below the ground state of the fissioning nucleus. Appropriate paths on the potential-energy surface of deformation have been found by theorists, but no physical grounds have been advanced that would allow the near equal populations we observe traveling each path. We suggest that this failure to find a reason for somewhat equal branching may be a fundamental flaw of current fission models. Assuming the proposed origins of these modes are correct, we conclude the low-energy, but also mass-symmetrical mode is likely to extend to far heavier nuclei. The high-energy mode will be restricted to a smaller region, a realm of nuclei defined by the proximity of the fragments to the strong neutron and proton shells in {sup 132}Sn.

  12. 75 FR 516 - Norfolk Southern Railway Company-Petition for Exemption-in Baltimore City and Baltimore County, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-05

    ... Cockeysville Industrial Track (``CIT''). The Line is located between railroad milepost UU-1.00 (located just... railroad milepost UU-15.44 in the City of Baltimore and in Baltimore County, MD. In addition to...

  13. Safety and efficacy of a backpassaged rMd5-delta-Meq vaccine virus in chickens

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Marek’s disease virus EcoR1-Q fragment of the viral genome encodes meq (MDV EcoQ) gene. Meq is an unique oncogene, present only in serotype 1 MDV and is consistently expressed in all latent or tumor cells. The meq gene was deleted from the very virulent rMd5 genome and was designated as rMd5-delta-M...

  14. An obesity-like gene MdTLP7 from apple (Malus × domestica) enhances abiotic stress tolerance.

    PubMed

    Du, Fan; Xu, Jia-Ning; Zhan, Chun-Yan; Yu, Zhi-Bo; Wang, Xiao-Yun

    2014-03-01

    Tubby-like proteins (TLPs) are found in a broad range of multicellular organisms. In mammals, genetic mutation of tubby or other TLPs can result in certain disease phenotypes related to animal specific characters: obesity, retinal degeneration, hearing loss, et al. Plants also harbor a large number of TLP genes, but the information in plants is far more limited. We identified a highly up-regulated obesity-like gene, MdTLP7, in our previous study of apple differential gene expression profile under chilling, indicating its possible role in plant abiotic stress tolerance. cDNA of MdTLP7 was amplified and expressed in Escherichia coli. In the solid and solution medium, the rate of growth and the quantity of the cell carrying MdTLP7 gene were significantly more than that of empty vector under salt and temperature stresses. To identify the functional region, serial deletion from both N-terminus and C-terminus of MdTLP7 was performed. In 415 amino acid polypeptide chain of MdTLP7, a middle conservative fragment (120-310 amino acid residues) played vital roles in stress tolerance. This fragment was involved in β barrel of Tubby domain according to the model of Tubby domain. All above results suggested MdTLP7 confers stress-tolerance to E. coli cell against abiotic stresses.

  15. Comparison of fission modes in {sup 252}Cf, {sup 257}Fm, and {sup 260}Md

    SciTech Connect

    van Aarle, J.; Siemon, K.; Patzelt, P.; Wild, J.F.; Lougheed, R.W.; Westmeier, W.

    1998-10-01

    Although the spontaneous-fission properties of heavy actinides have been studied for well over 35 years, many interesting and informative details continue to come into light. During the last decade, the spontaneous fission of {sup 252}Cf, {sup 257}Fm and {sup 260}Md has been extensively investigated at the Philipps University of Marburg (1{endash}4), by means of a gadolinium-doped liquid scintillation tank for neutron counting and surface barrier detectors for fission fragment detection. The three nuclides represent the transition from the well-known asymmetric fission yield distribution, as it is characteristic for {sup 252}Cf, to a much more symmetrical one, found in the fission of {sup 260}Md. Therefore, trends in the dynamical changes of fission properties have been derived from these studies. For the spontaneous fission of {sup 252}Cf and {sup 260}Md, it was already shown that different fission modes, as proposed by theoretical calculations of Brosa et al. (5), could be separated, using the correlation between the neutrons emitted in a fission event and both the observed fission-fragment mass and the total kinetic energy (1, 2). In the case of {sup 257}Fm, no theoretical calculations for fission modes exist. However, from the fission properties of the two surrounding actinides, one can expect at least three different fission modes, namely two {open_quotes}standard{close_quotes} and the {open_quotes}supershort{close_quotes} mode. In this paper, results from the recent {sup 257}Fm experiment will be presented and compared to systematics extracted from the fission properties of other heavy actinides. {copyright} {ital 1998 American Institute of Physics.}

  16. Sub-Terrahertz Spectroscopy of E.COLI Dna: Experiment, Statistical Model, and MD Simulations

    NASA Astrophysics Data System (ADS)

    Sizov, I.; Dorofeeva, T.; Khromova, T.; Gelmont, B.; Globus, T.

    2012-06-01

    We will present result of combined experimental and computational study of sub-THz absorption spectra from Escherichia coli (E.coli) DNA. Measurements were conducted using a Bruker FTIR spectrometer with a liquid helium cooled bolometer and a recently developed frequency domain sensor operating at room temperature, with spectral resolution of 0.25 cm-1 and 0.03 cm-1, correspondingly. We have earlier demonstrated that molecular dynamics (MD) simulation can be effectively applied for characterizing relatively small biological molecules, such as transfer RNA or small protein thioredoxin from E. coli , and help to understand and predict their absorption spectra. Large size of DNA macromolecules ( 5 million base pairs for E. coli DNA) prevents, however, direct application of MD simulation at the current level of computational capabilities. Therefore, by applying a second order Markov chain approach and Monte-Carlo technique, we have developed a new statistical model to construct DNA sequences from biological cells. These short representative sequences (20-60 base pairs) are built upon the most frequently repeated fragments (2-10 base pairs) in the original DNA. Using this new approach, we constructed DNA sequences for several non-pathogenic strains of E.coli, including a well-known strain BL21, uro-pathogenic strain, CFT073, and deadly EDL933 strain (O157:H7), and used MD simulations to calculate vibrational absorption spectra of these strains. Significant differences are clearly present in spectra of strains in averaged spectra and in all components for particular orientations. The mechanism of interaction of THz radiation with a biological molecule is studied by analyzing dynamics of atoms and correlation of local vibrations in the modeled molecule. Simulated THz vibrational spectra of DNA are compared with experimental results. With the spectral resolution of 0.1 cm-1 or better, which is now available in experiments, the very easy discrimination between different

  17. Understanding the mechanism of LCST phase separation of mixed ionic liquids in water by MD simulations.

    PubMed

    Zhao, Yuling; Wang, Huiyong; Pei, Yuanchao; Liu, Zhiping; Wang, Jianji

    2016-08-17

    Recently, it has been found experimentally that two different amino acid ionic liquids (ILs) can be mixed to show unique lowest critical solution temperature (LCST) phase separation in water. However, little is known about the mechanism of phase separation in these IL/water mixtures at the molecular level. In this work, five kinds of amino acid ILs were chosen to study the mechanism of LCST-type phase separation by molecular dynamics (MD) simulations. Toward this end, a series of all-atom MD simulations were carried out on the ternary mixtures consisting of two different ILs and water at different temperatures. The various interaction energies and radial distribution functions (RDFs) were calculated and analyzed for these mixed systems. It was found that for amino acid ILs, the -NH2 or -COOH group of one anion could have a hydrogen bonding interaction with the -COO(-) group of another anion. With the increase of temperature, this kind of hydrogen bonding interaction between anions was strengthened and then the anion-H2O electrostatic interaction was weakened, which led to the LCST-type phase separation of the mixed ILs in water. In addition, a series of MD simulations for [P6668]1[Lys]n[Asp]1-n/H2O systems were also performed to study the effect of the mixing ratio of ILs on phase separation. It was also noted that the experimental critical composition corresponding to the lowest critical solution temperature was well predicted from the total electrostatic interaction energies as a function of mole fraction of [P6668][Lys] in these systems. The conclusions drawn from this study may provide new insight into the LCST-type phase behavior of ILs in water, and motivate further studies on practical applications. PMID:27498928

  18. Ionic liquid induced dehydration and domain closure in lysozyme: FCS and MD simulation.

    PubMed

    Ghosh, Shirsendu; Parui, Sridip; Jana, Biman; Bhattacharyya, Kankan

    2015-09-28

    Effect of a room temperature ionic liquid (RTIL, [pmim][Br]) on the structure and dynamics of the protein, lysozyme, is investigated by fluorescence correlation spectroscopy (FCS) and molecular dynamic (MD) simulation. The FCS data indicate that addition of the RTIL ([pmim][Br]) leads to reduction in size and faster conformational dynamics of the protein. The hydrodynamic radius (rH) of lysozyme decreases from 18 Å in 0 M [pmim][Br] to 11 Å in 1.5 M [pmim][Br] while the conformational relaxation time decreases from 65 μs to 5 μs. Molecular origin of the collapse (size reduction) of lysozyme in aqueous RTIL is analyzed by MD simulation. The radial distribution function of water, RTIL cation, and RTIL anion from protein clearly indicates that addition of RTIL causes replacement of interfacial water by RTIL cation ([pmim](+)) from the first solvation layer of the protein providing a comparatively dehydrated environment. This preferential solvation of the protein by the RTIL cation extends up to ∼30 Å from the protein surface giving rise to a nanoscopic cage of overall radius 42 Å. In the nanoscopic cage of the RTIL (42 Å), volume fraction of the protein (radius 12 Å) is only about 2%. RTIL anion does not show any preferential solvation near protein surface. Comparison of effective radius obtained from simulation and from FCS data suggests that the "dry" protein (radius 12 Å) alone diffuses in a nanoscopic cage of RTIL (radius 42 Å). MD simulation further reveals a decrease in distance ("domain closure") between the two domains (alpha and beta) of the protein leading to a more compact structure compared to that in the native state. PMID:26429044

  19. Kinetics of CH4 and CO2 hydrate dissociation and gas bubble evolution via MD simulation.

    PubMed

    Uddin, M; Coombe, D

    2014-03-20

    Molecular dynamics simulations of gas hydrate dissociation comparing the behavior of CH4 and CO2 hydrates are presented. These simulations were based on a structurally correct theoretical gas hydrate crystal, coexisting with water. The MD system was first initialized and stabilized via a thorough energy minimization, constant volume-temperature ensemble and constant volume-energy ensemble simulations before proceeding to constant pressure-temperature simulations for targeted dissociation pressure and temperature responses. Gas bubble evolution mechanisms are demonstrated as well as key investigative properties such as system volume, density, energy, mean square displacements of the guest molecules, radial distribution functions, H2O order parameter, and statistics of hydrogen bonds. These simulations have established the essential similarities between CH4 and CO2 hydrate dissociation. The limiting behaviors at lower temperature (no dissociation) and higher temperature (complete melting and formation of a gas bubble) have been illustrated for both hydrates. Due to the shift in the known hydrate stability curves between guest molecules caused by the choice of water model as noted by other authors, the intermediate behavior (e.g., 260 K) showed distinct differences however. Also, because of the more hydrogen-bonding capability of CO2 in water, as reflected in its molecular parameters, higher solubility of dissociated CO2 in water was observed with a consequence of a smaller size of gas bubble formation. Additionally, a novel method for analyzing hydrate dissociation based on H-bond breakage has been proposed and used to quantify the dissociation behaviors of both CH4 and CO2 hydrates. Activation energies Ea values from our MD studies were obtained and evaluated against several other published laboratory and MD values. Intrinsic rate constants were estimated and upscaled. A kinetic reaction model consistent with macroscale fitted kinetic models has been proposed to

  20. The scope and variety of combined baccalaureate-MD programs in the United States.

    PubMed

    Eaglen, Robert H; Arnold, Louise; Girotti, Jorge A; Cosgrove, Ellen M; Green, Marianne M; Kollisch, Donald O; McBeth, Dani L; Penn, Mark A; Tracy, Sarah W

    2012-11-01

    The landscape of combined baccalaureate-MD programs has changed substantially in the last two decades but has not been documented in detail. The authors review the current state of these programs and discuss opportunities for future study of their evolving role and potential impact.In 2011, using a definition of baccalaureate-MD program built on prior research, the authors reviewed Association of American Medical Colleges sources and medical school Web sites to identify and characterize 81 active programs. In addition, they surveyed the 57 medical schools offering those programs; 31 schools with 39 programs responded. The resulting database inventories the number and distribution of programs; institutional affiliations; missions or goals; length; size; admissions criteria; curricula; and retention requirements.Since the inception of combined programs in 1961, their number and curricular length have increased. Pressures that spurred earlier programs remain evident in the goals of today's programs: attract talented high school or early college students, especially from diverse backgrounds; prepare physicians to meet societal needs; and offer an enriched premedical environment. Baccalaureate educational activities achieve program goals through special courses, medical experiences, community service, and learning communities tailored to students' needs. Admission and retention criteria are comparable to those of traditional medical schools.Combined baccalaureate-MD programs have evolved along several paths during the last half century and have enriched the baccalaureate experiences of medical students. Shifting expectations for the selection and education of future physicians warrant focused research on these programs to document their effectiveness in addressing those expectations. PMID:23018320

  1. A workshop on leadership for senior MD-PhD students.

    PubMed

    Meador, Catherine B; Parang, Bobak; Musser, Melissa A; Haliyur, Rachana; Owens, David A; Dermody, Terence S

    2016-01-01

    Leadership skills are essential for a successful career as a physician-scientist, yet many MD-PhD training programs do not offer formal training in leadership. The Vanderbilt Medical Scientist Training Program (MSTP) previously established a 2-day leadership workshop that has been held biennially since 2006 for students in the first and second years of the graduate school portion of combined MD and PhD training (G1/G2 students). Workshop attendees have consistently rated this workshop as a highly effective experience. However, opportunities for structured training in leadership competencies during the subsequent 3-5 years of MD-PhD training are limited. Given the success of the G1/G2 leadership workshop and the need for continuity in this model of leadership training, we developed a half-day workshop for MSTP students in the clinical years of medical school (M3/M4 students) to foster continued training in leadership. Our workshop curriculum, based in part on original cases drafted by Vanderbilt MSTP students, provides concrete strategies to manage conflict and navigate leadership transitions in the physician-scientist career path. The curriculum emphasizes both short-term competencies, such as effective participation as a member of a clinical team, and long-term competencies, such as leadership of a research team, division, or department. Our inaugural senior leadership workshop, held in August, 2015, was judged by student participants to be well organized and highly relevant to leadership concepts and skills. It will be offered biennially in our training curriculum for M3 and M4 MSTP students. PMID:27499363

  2. Atomic Mass and NuclearBinding Energy for Md-342(Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-342 (Mendelevium, atomic number Z = 101, mass number A = 342).

  3. Atomic Mass and NuclearBinding Energy for Md-340(Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-340 (Mendelevium, atomic number Z = 101, mass number A = 340).

  4. Atomic Mass and NuclearBinding Energy for Md-250(Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-250 (Mendelevium, atomic number Z = 101, mass number A = 250).

  5. Atomic Mass and NuclearBinding Energy for Md-248(Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-248 (Mendelevium, atomic number Z = 101, mass number A = 248).

  6. Atomic Mass and NuclearBinding Energy for Md-249(Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-249 (Mendelevium, atomic number Z = 101, mass number A = 249).

  7. Atomic Mass and NuclearBinding Energy for Md-257 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-257 (Mendelevium, atomic number Z = 101, mass number A = 257).

  8. Atomic Mass and NuclearBinding Energy for Md-255 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-255 (Mendelevium, atomic number Z = 101, mass number A = 255).

  9. Atomic Mass and NuclearBinding Energy for Md-258 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-258 (Mendelevium, atomic number Z = 101, mass number A = 258).

  10. Atomic Mass and NuclearBinding Energy for Md-251 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-251 (Mendelevium, atomic number Z = 101, mass number A = 251).

  11. Atomic Mass and NuclearBinding Energy for Md-252 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-252 (Mendelevium, atomic number Z = 101, mass number A = 252).

  12. Atomic Mass and NuclearBinding Energy for Md-256 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-256 (Mendelevium, atomic number Z = 101, mass number A = 256).

  13. Prompt neutron emission from the spontaneous fission of sup 260 Md

    SciTech Connect

    Wild, J.F.; van Aarle, J.; Westmeier, W.; Lougheed, R.W.; Hulet, E.K.; Moody, K.J.; Dougan, R.J.; Koop, E.; Glaser, R.E.; Brandt, R.; Patzelt, P. Philipps University, D-3550, Marburg an der Lahn, )

    1990-02-01

    We have made the first measurement of the number of neutrons emitted from the spontaneous fission of a nuclide in which very high fragment energies dominate the fission process. In bombardments of {sup 254}Es, we produced a large sample of 28-d {sup 260}Md, which was neutron counted in a 1-m-diameter spherical tank containing a Gd-doped scintillator solution. The average number of neutrons emitted per fission is only 2.58{plus minus}0.11, substantially less than for other actinides. A linear dependence of neutron multiplicity on fragment-excitation energy is observed to the highest values of total kinetic energy.

  14. Neutron emission as a function of fragment energy in the spontaneous fission of /sup 260/Md

    SciTech Connect

    Wild, J.F.; van Aarle, J.; Westmeiser, W.; Lougheed, R.W.; Hulet, E.K.; Moody, K.J.; Dougan, R.J.; Brandt, R.; Patzelt, P.

    1989-04-19

    We have made the first measurement of the number of neutrons emitted in the spontaneous fission of a nuclide in which very high fragment energies dominate the fission process. In bombardments of /sup 254/Es, we produced 28-d /sup 260/Md, which was neutron-counted in a 1-m-diam spherical tank containing a Gd-doped scintillator solution. The average number of neutrons emitted per fission is only 2.58 +- 0.11, substantially less than for other actinides. A direct correlation of neutron multiplicity with fragment excitation energy is clearly demonstrated. 3 refs., 5 figs.

  15. Constructing Atomic-Resolution RNA Structural Ensembles Using MD and Motionally Decoupled NMR RDCs

    PubMed Central

    Stelzer, Andrew C.; Frank, Aaron T.; Bailor, Maximillian H.; Andricioaei, Ioan; Al-Hashimi, Hashim M.

    2012-01-01

    A broad structural landscape often needs to be characterized in order to fully understand how regulatory RNAs perform their biological functions at the atomic level. We present a protocol for visualizing thermally accessible RNA conformations at atomic-resolution and with timescales extending up to milliseconds. The protocol combines molecular dynamics (MD) simulations with experimental residual dipolar couplings (RDCs) measured in partially aligned 13C/15N isotopically enriched elongated RNA samples. The structural ensembles generated in this manner provide insights into RNA dynamics and its role in functionally important transitions. PMID:19699798

  16. Human microbiome science: vision for the future, Bethesda, MD, July 24 to 26, 2013

    PubMed Central

    2014-01-01

    A conference entitled ‘Human microbiome science: Vision for the future’ was organized in Bethesda, MD from July 24 to 26, 2013. The event brought together experts in the field of human microbiome research and aimed at providing a comprehensive overview of the state of microbiome research, but more importantly to identify and discuss gaps, challenges and opportunities in this nascent field. This report summarizes the presentations but also describes what is needed for human microbiome research to move forward and deliver medical translational applications.

  17. The morning visit by Oliver Wendell Holmes, Sr., M.D.

    PubMed

    Ciric, Ivan S

    2009-10-01

    Today, physicians in general and neurosurgeons in particular practice in an era of fundamental change in Medicine from a profession to an industry. This change has produced erosion in the very personal physician-patient relationship. "The Morning Visit" is a poem written by Oliver Wendell Holmes, Sr., M.D., a 19th century physician, poet, and humanist. Although this beautiful poem was written approximately 150 years ago, it seems contemporarily relevant with its plea for the resurrection of the human touch in the behavior of physicians. It is in this spirit that this poem is shared with the readers of Neurosurgery. PMID:19834370

  18. Delivery of nitric oxide to the interior of mammalian cell by carbon nanotube: MD simulation.

    PubMed

    Raczyński, Przemysław; Górny, Krzysztof; Dawid, Aleksander; Gburski, Zygmunt

    2014-07-15

    Computer simulations have been performed to study the nanoindentation of phospholipid bilayer by the single-walled armchair carbon nanotube, filled with the nitric oxide molecules. The process has been simulated by means of molecular dynamics (MD) technique at physiological temperature T = 310 K with a constant pulling velocity of the nanotube. The force acting on the nanotube during membrane penetration has been calculated. We show that the indentation by carbon nanotube does not permanently destroy the membrane structure (self-sealing of the membrane occurs). The mobility of nitric oxide molecules during the membrane nanoindentation is discussed.

  19. Dynamics of biopolymers on nanomaterials studied by quasielastic neutron scattering and MD simulations

    NASA Astrophysics Data System (ADS)

    Dhindsa, Gurpreet K.

    Neutron scattering has been proved to be a powerful tool to study the dynamics of biological systems under various conditions. This thesis intends to utilize neutron scattering techniques, combining with MD simulations, to develop fundamental understanding of several biologically interesting systems. Our systems include a drug delivery system containing Nanodiamonds with nucleic acid (RNA), and two specific model proteins, beta-Casein and Inorganic Pyrophosphatase (IPPase). RNA and nanodiamond (ND) both are suitable for drug-delivery applications in nano-biotechnology. The architecturally flexible RNA with catalytic functionality forms nanocomposites that can treat life-threatening diseases. The non-toxic ND has excellent mechanical and optical properties and functionalizable high surface area, and thus actively considered for biomedical applications. In this thesis, we utilized two tools, quasielastic neutron scattering (QENS) and Molecular Dynamics Simulations to probe the effect of ND on RNA dynamics. Our work provides fundamental understanding of how hydrated RNA motions are affected in the RNA-ND nanocomposites. From the experimental and Molecular Dynamics Simulation (MD), we found that hydrated RNA motion is faster on ND surface than a freestanding one. MD Simulation results showed that the failure of Stokes Einstein relation results the presence of dynamic heterogeneities in the biomacromolecules. Radial pair distribution function from MD Simulation confirmed that the hydrophilic nature of ND attracts more water than RNA results the de-confinement of RNA on ND. Therefore, RNA exhibits faster motion in the presence of ND than freestanding RNA. In the second project, we studied the dynamics of a natively disordered protein beta-Casein which lacks secondary structures. In this study, the temperature and hydration effects on the dynamics of beta-Casein are explored by Quasielastic Neutron Scattering (QENS). We investigated the mean square displacement (MSD) of

  20. Full-Scale Crash Test of a MD-500 Helicopter with Deployable Energy Absorbers

    NASA Technical Reports Server (NTRS)

    Kellas, Sotiris; Jackson, Karen E.; Littell, Justin D.

    2010-01-01

    A new externally deployable energy absorbing system was demonstrated during a full-scale crash test of an MD-500 helicopter. The deployable system is a honeycomb structure and utilizes composite materials in its construction. A set of two Deployable Energy Absorbers (DEAs) were fitted on the MD-500 helicopter for the full-scale crash demonstration. Four anthropomorphic dummy occupants were also used to assess human survivability. A demonstration test was performed at NASA Langley's Landing and Impact Research Facility (LandIR). The test involved impacting the helicopter on a concrete surface with combined forward and vertical velocity components of 40-ft/s and 26-ft/s, respectively. The objectives of the test were to evaluate the performance of the DEA concept under realistic crash conditions and to generate test data for validation of dynamic finite element simulations. Descriptions of this test as well as other component and full-scale tests leading to the helicopter test are discussed. Acceleration data from the anthropomorphic dummies showed that dynamic loads were successfully attenuated to within non-injurious levels. Moreover, the airframe itself survived the relatively severe impact and was retested to provide baseline data for comparison for cases with and without DEAs.

  1. The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica).

    PubMed

    Pessina, Stefano; Angeli, Dario; Martens, Stefan; Visser, Richard G F; Bai, Yuling; Salamini, Francesco; Velasco, Riccardo; Schouten, Henk J; Malnoy, Mickael

    2016-10-01

    Varieties resistant to powdery mildew (PM; caused by Podosphaera leucotricha) are a major component of sustainable apple production. Resistance can be achieved by knocking-out susceptibility S-genes to be singled out among members of the MLO (Mildew Locus O) gene family. Candidates are MLO S-genes of phylogenetic clade V up-regulated upon PM inoculation, such as MdMLO11 and 19 (clade V) and MdMLO18 (clade VII). We report the knock-down through RNA interference of MdMLO11 and 19, as well as the complementation of resistance with MdMLO18 in the Arabidopsis thaliana triple mlo mutant Atmlo2/6/12. The knock-down of MdMLO19 reduced PM disease severity by 75%, whereas the knock-down of MdMLO11, alone or in combination with MdMLO19, did not result in any reduction or additional reduction of susceptibility compared with MdMLO19 alone. The test in A. thaliana excluded a role for MdMLO18 in PM susceptibility. Cell wall appositions (papillae) were present in both PM-resistant and PM-susceptible plants, but were larger in resistant lines. No obvious negative phenotype was observed in plants with mlo genes knocked down. Apparently, MdMLO19 plays the pivotal role in apple PM susceptibility and its knock-down induces a very significant level of resistance. PMID:26997489

  2. The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica).

    PubMed

    Pessina, Stefano; Angeli, Dario; Martens, Stefan; Visser, Richard G F; Bai, Yuling; Salamini, Francesco; Velasco, Riccardo; Schouten, Henk J; Malnoy, Mickael

    2016-10-01

    Varieties resistant to powdery mildew (PM; caused by Podosphaera leucotricha) are a major component of sustainable apple production. Resistance can be achieved by knocking-out susceptibility S-genes to be singled out among members of the MLO (Mildew Locus O) gene family. Candidates are MLO S-genes of phylogenetic clade V up-regulated upon PM inoculation, such as MdMLO11 and 19 (clade V) and MdMLO18 (clade VII). We report the knock-down through RNA interference of MdMLO11 and 19, as well as the complementation of resistance with MdMLO18 in the Arabidopsis thaliana triple mlo mutant Atmlo2/6/12. The knock-down of MdMLO19 reduced PM disease severity by 75%, whereas the knock-down of MdMLO11, alone or in combination with MdMLO19, did not result in any reduction or additional reduction of susceptibility compared with MdMLO19 alone. The test in A. thaliana excluded a role for MdMLO18 in PM susceptibility. Cell wall appositions (papillae) were present in both PM-resistant and PM-susceptible plants, but were larger in resistant lines. No obvious negative phenotype was observed in plants with mlo genes knocked down. Apparently, MdMLO19 plays the pivotal role in apple PM susceptibility and its knock-down induces a very significant level of resistance.

  3. Polyelectrolyte-promoted forward osmosis-membrane distillation (FO-MD) hybrid process for dye wastewater treatment.

    PubMed

    Ge, Qingchun; Wang, Peng; Wan, Chunfeng; Chung, Tai-Shung

    2012-06-01

    Polyelectrolytes have proven their advantages as draw solutes in forward osmosis process in terms of high water flux, minimum reverse flux, and ease of recovery. In this work, the concept of a polyelectrolyte-promoted forward osmosis-membrane distillation (FO-MD) hybrid system was demonstrated and applied to recycle the wastewater containing an acid dye. A poly(acrylic acid) sodium (PAA-Na) salt was used as the draw solute of the FO to dehydrate the wastewater, while the MD was employed to reconcentrate the PAA-Na draw solution. With the integration of these two processes, a continuous wastewater treatment process was established. To optimize the FO-MD hybrid process, the effects of PAA-Na concentration, experimental duration, and temperature were investigated. Almost a complete rejection of PAA-Na solute was observed by both FO and MD membranes. Under the conditions of 0.48 g mL(-1) PAA-Na and 66 °C, the wastewater was most efficiently dehydrated yet with a stabilized PAA-Na concentration around 0.48 g mL(-1). The practicality of PAA-Na-promoted FO-MD hybrid technology demonstrates not only its suitability in wastewater reclamation, but also its potential in other membrane-based separations, such as protein or pharmaceutical product enrichment. This study may provide the insights of exploring novel draw solutes and their applications in FO related processes.

  4. Diffusion of hydrocarbon in zeolite and effect due to pore topology: Neutron scattering and MD simulation studies

    NASA Astrophysics Data System (ADS)

    Mitra, S.; Sharma, V. K.; Chaplot, S. L.; Mukhopadhyay, R.

    2014-02-01

    Here we report detailed dynamical landscape of propylene adsorbed in ZSM5 and Na-Y zeolites as studied by neutron scattering and molecular dynamics (MD) simulation. Separation of propylene molecule from propane in the petrochemical industry is an important issue because it is one of the most demanding energetic separation processes due to their very close relative volatilities and molecular sizes. Aim here is to investigate the correlation of the host topology towards the dynamics of guest molecules. ZSM5 zeolite is typified by a network of intersecting channels while Na-Y has a network of spherical supercages interconnected by windows. Both neutron scattering and MD simulation studies indicated that translational diffusion of propylene is more restricted in ZSM5 compared to Na-Y zeolite. Fully atomistic MD simulation studies showed that the translation involves three different time scales and rotational motion of the propylene is much faster than translation. The observed dynamics in QENS spectrometer (ΔE ˜ 200 μeV) corresponds to one of the three translational components indicated in the MD simulation. The faster rotational motion is observed in a wider energy window spectrometer (ΔE ˜ 3 meV). MD simulation results also show some interesting features like, non-isotropic rotation in ZSM5 while it has been isotropic in Na-Y zeolite. It is also found that propylene molecules prefer to orient along channels of ZSM5 zeolite.

  5. Detection of germline BRCA1 mutations by Multiple-Dye Cleavase Fragment Length Polymorphism (MD-CFLP) method

    PubMed Central

    Casadei, S; Cortesi, L; Pensotti, V; Radice, P; Pierotti, M; Amadori, D; Calistri, D

    2001-01-01

    We describe the Multiple-Dye Cleavase Fragment Length Polymorphism (MD-CFLP) method set up for a sensitive and preliminary rapid screening of BRCA1 mutations. We analysed exons 11 and 16, which are known to cover slightly more than 70% of the whole coding region of the gene, subdivided into 4 amplicons and labelled with different fluorescent dUTPs. MD-CFLP was first utilised on a panel of 30 DNA samples in which the presence of single-base substitutions or small deletions/insertions had been previously identified by direct sequencing as gold standard, in order to define the optimal conditions in terms of PCR amplification and temperature of digestion. In a second step, we blindly analysed 21 DNA samples by MD-CFLP to verify its reliability. The sensitivity and specificity of MD-CFLP were both 100% in the first study, and 80% and 94%, respectively, in the blind sample assay. Our results demonstrate the capability of the MD-CFLP method to detect DNA sequence alterations in fragments of more than 1 kb. We conclude that CFLP is a powerful tool in mutational analysis, offering reliable results in a shorter time and at a lower cost than conventional methods, and its potential can be enhanced when internal fluorescent labelling and laser detection are used. © 2001 Cancer Research Campaignhttp://www.bjcancer.com PMID:11556835

  6. Structural and dynamical aspects of Streptococcus gordonii FabH through molecular docking and MD simulations.

    PubMed

    Shamim, Amen; Abbasi, Sumra Wajid; Azam, Syed Sikander

    2015-07-01

    β-Ketoacyl-ACP-synthase III (FabH or KAS III) has become an attractive target for the development of new antibacterial agents which can overcome the multidrug resistance. Unraveling the fatty acid biosynthesis (FAB) metabolic pathway and understanding structural coordinates of FabH will provide valuable insights to target Streptococcus gordonii for curing oral infection. In this study, we designed inhibitors against therapeutic target FabH, in order to block the FAB pathway. As compared to other targets, FabH has more interactions with other proteins, located on the leading strand with higher codon adaptation index value and associated with lipid metabolism category of COG. Current study aims to gain in silico insights into the structural and dynamical aspect of S. gordonii FabH via molecular docking and molecular dynamics (MD) simulations. The FabH protein is catalytically active in dimerization while it can lock in monomeric state. Current study highlights two residues Pro88 and Leu315 that are close to each other by dimerization. The active site of FabH is composed of the catalytic triad formed by residues Cys112, His249, and Asn279 in which Cys112 is involved in acetyl transfer, while His249 and Asn279 play an active role in decarboxylation. Docking analysis revealed that among the studied compounds, methyl-CoA disulfide has highest GOLD score (82.75), binding affinity (-11 kcal/mol) and exhibited consistently better interactions. During MD simulations, the FabH structure remained stable with the average RMSD value of 1.7 Å and 1.6 Å for undocked protein and docked complex, respectively. Further, crucial hydrogen bonding of the conserved catalytic triad for exhibiting high affinity between the FabH protein and ligand is observed by RDF analysis. The MD simulation results clearly demonstrated that binding of the inhibitor with S. gordonii FabH enhanced the structure and stabilized the dimeric FabH protein. Therefore, the inhibitor has the potential to become

  7. Estimation of Hydrogen-Exchange Protection Factors from MD Simulation Based on Amide Hydrogen Bonding Analysis.

    PubMed

    Park, In-Hee; Venable, John D; Steckler, Caitlin; Cellitti, Susan E; Lesley, Scott A; Spraggon, Glen; Brock, Ansgar

    2015-09-28

    Hydrogen exchange (HX) studies have provided critical insight into our understanding of protein folding, structure, and dynamics. More recently, hydrogen exchange mass spectrometry (HX-MS) has become a widely applicable tool for HX studies. The interpretation of the wealth of data generated by HX-MS experiments as well as other HX methods would greatly benefit from the availability of exchange predictions derived from structures or models for comparison with experiment. Most reported computational HX modeling studies have employed solvent-accessible-surface-area based metrics in attempts to interpret HX data on the basis of structures or models. In this study, a computational HX-MS prediction method based on classification of the amide hydrogen bonding modes mimicking the local unfolding model is demonstrated. Analysis of the NH bonding configurations from molecular dynamics (MD) simulation snapshots is used to determine partitioning over bonded and nonbonded NH states and is directly mapped into a protection factor (PF) using a logistics growth function. Predicted PFs are then used for calculating deuteration values of peptides and compared with experimental data. Hydrogen exchange MS data for fatty acid synthase thioesterase (FAS-TE) collected for a range of pHs and temperatures was used for detailed evaluation of the approach. High correlation between prediction and experiment for observable fragment peptides is observed in the FAS-TE and additional benchmarking systems that included various apo/holo proteins for which literature data were available. In addition, it is shown that HX modeling can improve experimental resolution through decomposition of in-exchange curves into rate classes, which correlate with prediction from MD. Successful rate class decompositions provide further evidence that the presented approach captures the underlying physical processes correctly at the single residue level. This assessment is further strengthened in a comparison of

  8. Molecular dynamics (MD) studies on phase transformation and deformation behaviors in FCC metals and alloys

    NASA Astrophysics Data System (ADS)

    Qi, Yue

    This thesis focused on the phase transformation and deformation behaviors in face center cubic (FCC) metals and alloys. These studies used the new quantum modified Sutton-Chen (QMSC) many-body potentials for Cu, Ni, Ag, and Au and for their alloys through simple combination rules. Various systems and processes are simulated by standard equilibrium molecular dynamics (MD), quasi-static equilibrium MD and non-equilibrium MD (NEMD), cooperated with different periodic boundary conditions. The main topics include: (1) Melting, glass formation, and crystallization processes in bulk alloys. In our simulation CuNi and pure Cu always form an FCC crystal, while Cu4Ag6 always forms glass (with Tg decreasing as the quench rate increases) due to the large atomic size difference. (2) Size effects in melting and crystallization in Ni nano clusters. There is a transition from cluster or molecular regime (where the icosahedral is the stable structure) below ˜500 atoms to a mesoscale regime (with well-defined bulk and surface properties and surface melting processes, which leads to Tm,N = Tm,B - alpha N-1/3) above ˜750 atoms. (3) The deformation behavior of metallic nanowires of pure Ni, NiCu and NiAu alloys, under high rates of uniaxial tensile strain, ranging from 5*108/s to 5*1010/s. We find that deformation proceeds through twinning and coherent slipping at low strain rate and amorphization at high strain rate. This research provides a new method, fast straining, to induce amorphization except fast cooling and disordering. (4) The calculation of the ½ <110> screw dislocation in nickel (Ni). We calculated the core energy of screw dislocation after dissociation is 0.5 eV/b, the annihilation process of opposite signed dislocations depends dramatically on the configurations of dissociation planes and the cross-slip energy barrier is 0.1eV/b. (5) Friction anisotropy on clean Ni(100)/(100) interface. We found that static friction coefficient on flat and incommensurate interface is

  9. Steric effects on intramolecular reactivity in cyclic dipeptides: Conformational analysis validated by a combined MD/DFT approach

    NASA Astrophysics Data System (ADS)

    Lewandowska, A.; Carmichael, I.; Hörner, G.; Hug, G. L.; Marciniak, B.

    2011-08-01

    The present Molecular Dynamics (MD) simulation study addresses the geometric requirements of close-contact formation in short peptides. This process, that is probed herein by intramolecular H-atom transfer, initiated by triplet-excited ketones, demands close contact between the H-donating and H-accepting moieties. Thus, any deduction about the compound's reactivity based just on MD simulations, requires independent verification of the computed conformational preferences. In this study, a procedure was developed using diketopiperazine-linked benzophenone/tyrosine dyads. Specifically, it involves a comparison of the dyads' experimental 3J(H α-H β(a/b)) spin-spin coupling constants with the theoretical values obtained by weighting DFT-computed spin-spin coupling constants with the MD-computed probability distributions for the dyads' configurations.

  10. Screening of UV-B-induced genes from apple peels by SSH: possible involvement of MdCOP1-mediated signaling cascade genes in anthocyanin accumulation.

    PubMed

    Peng, Ting; Saito, Takanori; Honda, Chikako; Ban, Yusuke; Kondo, Satoru; Liu, Ji-Hong; Hatsuyama, Yoshimichi; Moriguchi, Takaya

    2013-07-01

    Suppression subtractive hybridization (SSH) was employed to identify candidate genes involved in red coloration in apple peel with the ultraviolet (UV)-B-treated 'Mutsu'. After reverse Northern blotting verification, nearly 80 clones were successfully sequenced. Large portions of the expressed sequence tags (ESTs) are well characterized anthocyanin biosynthesis-related genes, such as chalcone synthase (11A5), flavonol synthase (12F3), anthocyanidin synthase (11H5) and UDP-glycosyl transferase (14A12) whose presence proved the success of SSH. Eight ESTs were selected for quantitative real-time polymerase chain reaction analysis and their expressions were all elevated in 'Induction', further confirming the reliability of the SSH library. One EST, 11F4 (CONSTITUTIVE PHOTOMORPHOGENIC 1: COP1) with putative function in light signal relay was further analyzed in 'Mutsu' and 'Tsugaru', along with MdHY5 (ELONGATED HYPOCOTYL 5: the downstream target of COP1), MdMYB22 (a possible flavonol-specific activator under the regulation of HY5, belonging to the SG7/PRODUCTION OF FLAVONOL GLYCOSIDES family) and MdMYBA. Results showed that MdCOP1, MdHY5, MdMYB22 and MdMYBA were all UV-B inducible genes and anthocyanin accumulation occurred after their increased expressions. Moreover, their expressions and anthocyanin content were enhanced under UV-B plus 17°C treatment. The presence of G box, a known consensus binding site of HY5, in the MdMYBA promoter region implicated that it could be regulated by MdHY5, which was verified by the result of the yeast one-hybrid analysis. Our data suggested that UV-B irradiation would induce the utmost upstream light signaling factor, MdCOP1, which activates MdHY5 signaling by binding to the promoter regions of MdMYBs, and finally leads to the red coloration of apple peels.

  11. Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10.

    PubMed

    Espley, Richard V; Hellens, Roger P; Putterill, Jo; Stevenson, David E; Kutty-Amma, Sumathi; Allan, Andrew C

    2007-02-01

    Anthocyanin concentration is an important determinant of the colour of many fruits. In apple (Malus x domestica), centuries of breeding have produced numerous varieties in which levels of anthocyanin pigment vary widely and change in response to environmental and developmental stimuli. The apple fruit cortex is usually colourless, although germplasm does exist where the cortex is highly pigmented due to the accumulation of either anthocyanins or carotenoids. From studies in a diverse array of plant species, it is apparent that anthocyanin biosynthesis is controlled at the level of transcription. Here we report the transcript levels of the anthocyanin biosynthetic genes in a red-fleshed apple compared with a white-fleshed cultivar. We also describe an apple MYB transcription factor, MdMYB10, that is similar in sequence to known anthocyanin regulators in other species. We further show that this transcription factor can induce anthocyanin accumulation in both heterologous and homologous systems, generating pigmented patches in transient assays in tobacco leaves and highly pigmented apple plants following stable transformation with constitutively expressed MdMYB10. Efficient induction of anthocyanin biosynthesis in transient assays by MdMYB10 was dependent on the co-expression of two distinct bHLH proteins from apple, MdbHLH3 and MdbHLH33. The strong correlation between the expression of MdMYB10 and apple anthocyanin levels during fruit development suggests that this transcription factor is responsible for controlling anthocyanin biosynthesis in apple fruit; in the red-fleshed cultivar and in the skin of other varieties, there is an induction of MdMYB10 expression concurrent with colour formation during development. Characterization of MdMYB10 has implications for the development of new varieties through classical breeding or a biotechnological approach.

  12. Measuring service quality at the University of Texas M.D. Anderson Cancer Center.

    PubMed

    Anderson, E A; Zwelling, L A

    1996-01-01

    Evaluates the service quality of four clinics at the University of Texas M.D. Anderson Cancer Center using a questionnaire methodology. The SERVQUAL instrument was administered to patients of the Medical Breast, Leukemia, Medical Gastroenterology and Bone Marrow Aspiration clinics. Results show that, according to the service gap methodology of comparing expectations and perceptions, across all four clinics the issues of billing accuracy and waiting times are deemed by patients as significant problems. In comparing the individual clinics, the Medical Gastroenterology and Leukemia clinics are best performers and the Medical Breast clinic is the worst. However, these differences in performance are due to differences in patients' expectations of service quality, rather than differences in perceptions. Concludes that customer expectations can have a strong impact on a firm's evaluation of its service quality. PMID:10162922

  13. Three-phase permeabilities and other characteristics of 260-mD fired Berea

    SciTech Connect

    Maloney, D.; Brinkmeyer, A.

    1992-04-01

    A laboratory investigation was conducted to determine relative permeabilities and other characteristics of a 260-mD fired Berea sandstone. The mineralogical and physical characteristics of the sample were characterized by XRD tests, thin section analyses, mercury injection tests, and centrifuge capillary pressure and wettability tests. Two-phase oil/water relative permeabilities were measured under several stress conditions. Resistivity characteristics of the sample were also evaluated during several of the oil/water tests. Oil/gas and gas/water relative permeabilities were measured during steady-state tests. Three-phase steady-state oil/gas/water tests were performed for six DDI saturation trajectories (decreasing brine and oil saturations, increasing gas saturation) in which the sample was not cleaned between saturation trajectories.

  14. Prediction of drug-packaging interactions via molecular dynamics (MD) simulations.

    PubMed

    Feenstra, Peter; Brunsteiner, Michael; Khinast, Johannes

    2012-07-15

    The interaction between packaging materials and drug products is an important issue for the pharmaceutical industry, since during manufacturing, processing and storage a drug product is continuously exposed to various packaging materials. The experimental investigation of a great variety of different packaging material-drug product combinations in terms of efficacy and safety can be a costly and time-consuming task. In our work we used molecular dynamics (MD) simulations in order to evaluate the applicability of such methods to pre-screening of the packaging material-solute compatibility. The solvation free energy and the free energy of adsorption of diverse solute/solvent/solid systems were estimated. The results of our simulations agree with experimental values previously published in the literature, which indicates that the methods in question can be used to semi-quantitatively reproduce the solid-liquid interactions of the investigated systems.

  15. Static and dynamic properties of confined, cold ion plasmas: MD (molecular dynamics) simulations

    SciTech Connect

    Schiffer, J.P.

    1989-01-01

    Some four years ago it was suggested that in the new generation of heavy ion accelerator storage rings for multiply charged ions, being planned in Europe, one may well attain internal temperatures that would correspond to very cold plasmas. Since that time, the techniques of electron or laser cooling of such beams has evolved and it may well be possible to reach temperatures corresponding to a plasma coupling parameter {Gamma} >> 100. I was fortunate to have had an opportunity to collaborate during 1986-87 with my former colleague Aneesur Rahman, of Molecular Dynamics fame, and we adapted the MD method to the calculation of the properties of cold confined plasmas. After Rahman's premature death two years ago I have continued the exploration of these systems and would like to summarize the results here. 9 refs., 10 figs.

  16. Mary Edwards Walker, M.D.: a feminist physician a century ahead of her time.

    PubMed

    Spiegel, A D; Suskind, P B

    1996-06-01

    In her teens, Mary Edwards Walker already wore the "bloomer" outfit began to campaign for reforming the "unhygienic" clothing of women. Assertively, she attended medical school and earned her M.D. degree. Due to prejudice, her practice did not flourish and she moved to Washington to offer her medical services to the Union as the Civil War began. Rebuffed by the male medical bureaucrats, she volunteered her services anyway. Eventually, she was awarded the Congressional Medal of Honor, the only women to ever gain such distinction. After the war, Walker became a journalist, an author of two sensational books, a political lobbyist, a suffrage campaigner, a professional and public lecturer, an ardent dress reformer, a peace activist, a Utopianist and a women's right advocate. Light-years ahead of her times, Dr. Walker was an intelligent, independent, irrepressible and indefatigable proponent for a host of worthy causes.

  17. Study of delayed fission of the isotopes of Bk, Es, and Md

    SciTech Connect

    Gangrskii, Y.P.; Miller, M.B.; Mikhailov, L.V.; Kharisov, I.F.

    1980-02-01

    We have measured the probabilities of delayed fission in electron capture for the nuclei /sup 240,242/Bk, /sup 244,246,248/Es, and /sup 248,250/Md. The data are analyzed by means of analytical expressions obtained in the work which explicitly relate the probability of delayed fission (in electron capture or ..beta../sup -/ decay) to the parameters of a two-humped fission barrier. As a result of the analysis the fission barriers are evaluated for the corresponding daughter nuclei: the isotopes of Cm, Cf, and Fm. According to the estimates the height of the fission barrier for the group of nuclei investigated is close to the value 6 MeV and does not decrease appreciably with increase of the Z of the nucleus or with removal from the ..beta..-stability band.

  18. Frank H. Netter, M.D. (1906-1991): the artist and his legacy.

    PubMed

    Hansen, John T

    2006-09-01

    The renowned medical illustrator Frank H. Netter, M.D., was born on 25 April 1906 in Brooklyn, NY, and died on 17 September 1991 in New York, NY, at the age of 85. In his professional career, Dr. Netter painted over 4,000 medical illustrations, many of which became the center pieces of over 250 issues of Clinical Symposia and were compiled in the 13 volume set of The CIBA Collection of Medical Illustrations. Two years prior to his death, the Atlas of Human Anatomy was published, considered by many to be Dr. Netter's crowning achievement. The Atlas rapidly became the most widely used atlas of anatomy in American medical schools and currently is published in 16 languages. Netter's impressive career as an illustrator and his unique style and legacy to medical education are summarized briefly in this review.

  19. Fluorescence of PRODAN in Water: a Computational QM/MM MD Study

    SciTech Connect

    Pederzoli, Marek; Sobek, Lukas; Brabec, Jiri; Kowalski, Karol; Cwiklik, Lukasz; Pittner, Jiri

    2014-03-28

    Fluorescent properties of PRODAN (6-propionyl-2-dimethylaminonaphthalene) in water were studied by means of excited state molecular dynamics simulations employing a combined quantum mechanical and molecular mechanical approach with the time-dependent density functional theory (TD-DFT QM/MM MD). The state of the art coupled cluster method was used to benchmark density functional theory calculations. The influence of the water environment on PRODAN emission was investigated by employing several computational schemes with varying description of the solvent. The issue of the molecular geometry of the excited state PRODAN molecule in water was addressed. The experimental emission spectrum was reproduced for the planar excited state conformer of PRODAN in the extended environment of 300 explicit water molecules. The planar conformer was shown to be predominantly responsible for fluorescence. The twisted isomer is strongly stabilized in water, but rapidly evolve towards a conical intersection, and hence the twisted conformer is fluorescently inactive.

  20. Measuring service quality at the University of Texas M.D. Anderson Cancer Center.

    PubMed

    Anderson, E A; Zwelling, L A

    1996-01-01

    Evaluates the service quality of four clinics at the University of Texas M.D. Anderson Cancer Center using a questionnaire methodology. The SERVQUAL instrument was administered to patients of the Medical Breast, Leukemia, Medical Gastroenterology and Bone Marrow Aspiration clinics. Results show that, according to the service gap methodology of comparing expectations and perceptions, across all four clinics the issues of billing accuracy and waiting times are deemed by patients as significant problems. In comparing the individual clinics, the Medical Gastroenterology and Leukemia clinics are best performers and the Medical Breast clinic is the worst. However, these differences in performance are due to differences in patients' expectations of service quality, rather than differences in perceptions. Concludes that customer expectations can have a strong impact on a firm's evaluation of its service quality.