17. Underside of bridge and abutment with large boulder looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. Underside of bridge and abutment with large boulder looking ENE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

13. Roadway and place of a thousand drips looking ESE. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Roadway and place of a thousand drips looking ESE. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

15. Culvert and corrugated pipe with place of a thousand ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. Culvert and corrugated pipe with place of a thousand drips in background looking S. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

7. DETAIL CENTRAL PIER (SKEWBACK) WITH BREAKWATER, UPSTREAM (EAST) SIDE. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. DETAIL CENTRAL PIER (SKEWBACK) WITH BREAKWATER, UPSTREAM (EAST) SIDE. NOTE FRACTURES ALONG BARREL ARCH EXTRADOS. - Roaring Creek Bridge, State Road 2005 spanning Roaring Creek in Locust Township, Slabtown, Columbia County, PA

Environmentally friendly education: A passive solar, straw-bale school

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stone, L.; Dickinson, J.

The Waldorf students in the Roaring Fork Valley of western Colorado are learning their reading, writing and arithmetic in the cozy confines of a solar heated, naturally lit, straw-bale school. The Waldorf education system, founded in 1919 by Austrian Rudolph Steiner, stresses what's appropriate for the kids, not what's easiest to teach. In constructing a new school, the Waldorf community wanted a building that would reflect their philosophy. There was a long list of requirements: natural, energy efficient, light, warm, alive, and earthy. Passive solar straw-bale construction brought together all those qualities.

Bed-material entrainment potential, Roaring Fork River at Basalt, Colorado

USGS Publications Warehouse

Elliott, John G.

2002-01-01

The Roaring Fork River at Basalt, Colorado, has a frequently mobile streambed composed of gravel, cobbles, and boulders. Recent urban and highway development on the flood plain, earlier attempts to realign and confine the channel, and flow obstructions such as bridge openings and piers have altered the hydrology, hydraulics, sediment transport, and sediment deposition areas of the Roaring Fork. Entrainment and deposition of coarse sediment on the streambed and in large alluvial bars have reduced the flood-conveying capacity of the river. Previous engineering studies have identified flood-prone areas and hazards related to inundation and high streamflow velocity, but those studies have not evaluated the potential response of the channel to discharges that entrain the coarse streambed. This study builds upon the results of earlier flood studies and identifies some potential areas of concern associated with bed-material entrainment. Cross-section surveys and simulated water-surface elevations from a previously run HEC?RAS model were used to calculate the boundary shear stress on the mean streambed, in the thalweg, and on the tops of adjacent alluvial bars for four reference streamflows. Sediment-size characteristics were determined for surficial material on the streambed, on large alluvial bars, and on a streambank. The median particle size (d50) for the streambed samples was 165 millimeters and for the alluvial bars and bank samples was 107 millimeters. Shear stresses generated by the 10-, 50-, and 100-year floods, and by a more common flow that just inundated most of the alluvial bars in the study reach were calculated at 14 of the cross sections used in the Roaring Fork River HEC?RAS model. The Shields equation was used with a Shields parameter of 0.030 to estimate the critical shear stress for entrainment of the median sediment particle size on the mean streambed, in the thalweg, and on adjacent alluvial bar surfaces at the 14 cross sections. Sediment-entrainment potential for a specific geomorphic surface was expressed as the ratio of the flood-generated boundary shear stress to the critical shear stress (to/tc) with respect to two threshold conditions. The partial entrainment threshold (to/tc=1) is the condition where the mean boundary shear stress (to) equals the critical shear stress for the median particle size (tc) at that cross section. At this threshold discharge, the d50 particle size becomes entrained, but movement of d50-size particles may be limited to a few individual particles or in a small area of the streambed surface. The complete entrainment threshold (to/tc=2) is the condition where to is twice the critical shear stress for the median particle size, the condition where complete or widespread mobilization of the d50 particle-size fraction is anticipated. Entrainment potential for a specific reference streamflow varied greatly in the downstream direction. At some cross sections, the bed or bar material was mobile, whereas at other cross sections, the bed or bar material was immobile for the same discharge. The significance of downstream variability is that sediment entrained at one cross section may be transported into, but not through, a cross section farther downstream, a situation resulting in sediment deposition and possibly progressive aggradation and loss of channel conveyance. Little or no sediment in the d50-size range is likely to be entrained or transported through much of the study reach by the bar-inundating streamflow. However, the entrainment potential at this discharge increases abruptly to more than twice the critical value, then decreases abruptly, at a series of cross sections located downstream from the Emma and Midland Avenue Bridges. Median particle-size sediment is mobile at most cross sections in the study reach during the 10-year flood; however, the bed material is immobile at cross sections just upstream from the Upper Bypass and Midland Avenue Bridges. A similar s

INDIAN PEAKS WILDERNESS, COLORADO.

USGS Publications Warehouse

Pearson, Robert C.; Speltz, Charles N.

1984-01-01

The Indian Peaks Wilderness northwest of Denver is partly within the Colorado Mineral Belt, and the southeast part of it contains all the geologic characteristics associated with the several nearby mining districts. Two deposits have demonstrated mineral resources, one of copper and the other of uranium; both are surrounded by areas with probable potential. Two other areas have probable resource potential for copper, gold, and possibly molydenum. Detailed gravity and magnetic studies in the southeast part of the Indian Peaks Wilderness might detect in the subsurface igneous bodies that may be mineralized. Physical exploration such as drilling would be necessary to determine more precisely the copper resources at the Roaring Fork locality and uranium resources at Wheeler Basin.

Response of red deer stags ( Cervus elaphus) to playback of harsh versus common roars

NASA Astrophysics Data System (ADS)

Garcia, Maxime; Wyman, Megan T.; Charlton, Benjamin D.; Tecumseh Fitch, W.; Reby, David

2014-10-01

Red deer stags ( Cervus elaphus) give two distinct types of roars during the breeding season, the "common roar" and the "harsh roar." Harsh roars are more frequent during contexts of intense competition, and characterized by a set of features that increase their perceptual salience, suggesting that they signal heightened arousal. While common roars have been shown to encode size information and mediate both male competition and female choice, to our knowledge, the specific function of harsh roars during male competition has not yet been studied. Here, we investigate the hypothesis that the specific structure of male harsh roars signals high arousal to competitors. We contrast the behavioral responses of free ranging, harem-holding stags to the playback of harsh roars from an unfamiliar competitor with their response to the playback of common roars from the same animal. We show that males react less strongly to sequences of harsh roars than to sequences of common roars, possibly because they are reluctant to escalate conflicts with highly motivated and threatening unfamiliar males in the absence of visual information. While future work should investigate the response of stags to harsh roars from familiar opponents, our observations remain consistent with the hypothesis that harsh roars may signal motivation during male competition, and illustrate how intrasexual selection can contribute to the diversification of male vocal signals.

Geological hazards, vulnerability, and risk assessment using GIS: model for Glenwood Springs, Colorado

NASA Astrophysics Data System (ADS)

Mejía-Navarro, Mario; Wohl, Ellen E.; Oaks, Sherry D.

1994-08-01

Glenwood Springs, Colorado, lies at the junction of the Roaring Fork and Colorado Rivers, surrounded by the steep peaks of the Colorado Rocky Mountains. Large parts of the region have had intensive sheet erosion, debris flows, and hyperconcentrated floods triggered by landslides and slumps. The latter come from unstable slopes in the many tributary channels on the mountainsides, causing concentration of debris in channels and a large accumulation of sediment in colluvial wedges and debris fans that line the river valleys. Many of the landslide and debris-flow deposits exist in a state resembling suspended animation, ready to be destabilized by intense precipitation and/or seismic activity. During this century urban development in the Roaring Fork River valley has increased rapidly. The city of Glenwood Springs continues to expand over unstable debris fans without any construction of hazard mitigation structures. Since 1900, Glenwood Springs has had at least 21 damaging debris flows and floods; on July 24, 1977 a heavy thunderstorm spread a debris flow over more than 80 ha of the city. This paper presents a method that uses Geographic Information Systems (GIS) to assess geological hazards, vulnerability, and risk in the Glenwood Springs area. The hazards evaluated include subsidence, rockfall, debris flows, and floods, and in this paper we focus on debris flows and subsidence. Information on topography, hydrology, precipitation, geomorphic processes, bedrock and surficial geology, structural geology, soils, vegetation, and land use, was processed for hazard assessment using a series of algorithms. ARC/INFO and GRASS GIS softwares were used to produce maps and tables in a format accessible to urban planners. After geological hazards were defined for the study area, we estimated the vulnerability ( Ve) of various elements for an event of intensity i. Risk is assessed as a function of hazard and vulnerability. We categorized the study area in 14 classes for planning procedures; 7 classes defined as areas suitable for human settlement, and 7 classes defined as unsuitable for building, and most effectively reserved for parks and forests.

Lion, ungulate, and visitor reactions to playbacks of lion roars at Zoo Atlanta.

PubMed

Kelling, Angela S; Allard, Stephanie M; Kelling, Nicholas J; Sandhaus, Estelle A; Maple, Terry L

2012-01-01

Felids in captivity are often inactive and elusive in zoos, leading to a frustrating visitor experience. Eight roars were recorded from an adult male lion and played back over speakers as auditory enrichment to benefit the lions while simultaneously enhancing the zoo visitor experience. In addition, ungulates in an adjacent exhibit were observed to ensure that the novel location and increased frequency of roars did not lead to a stress or fear response. The male lion in this study roared more in the playback phase than in the baseline phases while not increasing any behaviors that would indicate compromised welfare. In addition, zoo visitors remained at the lion exhibit longer during playback. The nearby ungulates never exhibited any reactions stronger than orienting to playbacks, identical to their reactions to live roars. Therefore, naturalistic playbacks of lion roars are a potential form of auditory enrichment that leads to more instances of live lion roars and enhances the visitor experience without increasing the stress levels of nearby ungulates or the lion themselves, who might interpret the roar as that of an intruder.

The carpenter fork bed, a new - and older - Black-shale unit at the base of the New Albany shale in central Kentucky: Characterization and significance

USGS Publications Warehouse

Barnett, S.F.; Ettensohn, F.R.; Norby, R.D.

1996-01-01

Black shales previously interpreted to be Late Devonian cave-fill or slide deposits are shown to be much older Middle Devonian black shales only preserved locally in Middle Devonian grabens and structural lows in central Kentucky. This newly recognized - and older -black-shale unit occurs at the base of the New Albany Shale and is named the Carpenter Fork Bed of the Portwood Member of the New Albany Shale after its only known exposure on Carpenter Fork in Boyle County, central Kentucky; two other occurrences are known from core holes in east-central Kentucky. Based on stratigraphic position and conodont biostratigraphy, the unit is Middle Devonian (Givetian: probably Middle to Upper P. varcus Zone) in age and occurs at a position represented by an unconformity atop the Middle Devonian Boyle Dolostone and its equivalents elsewhere on the outcrop belt. Based on its presence as isolated clasts in the overlying Duffin Bed of the Portwood Member, the former distribution of the unit was probably much more widespread - perhaps occurring throughout western parts of the Rome trough. Carpenter Fork black shales apparently represent an episode of subsidence or sea-level rise coincident with inception of the third tectophase of the Acadian orogeny. Deposition, however, was soon interrupted by reactivation of several fault zones in central Kentucky, perhaps in response to bulge migration accompanying start of the tectophase. As a result, much of central Kentucky was uplifted and tilted, and the Carpenter Fork Bed was largely eroded from the top of the Boyle, except in a few structural lows like the Carpenter Fork graben where a nearly complete record of Middle to early Late Devonian deposition is preserved.

Development of a Flood-Warning System and Flood-Inundation Mapping in Licking County, Ohio : Executive Summary Report

DOT National Transportation Integrated Search

2012-04-01

Licking County, Ohio, has experienced numerous floods with the majority of flood damages occurring in the central and south-central areas of the county along four streams: the Licking River, North Fork Licking River, South Fork Licking River, and Rac...

First Observations of 5fce Auroral Roar Emissions

NASA Astrophysics Data System (ADS)

Labelle, J. W.

2012-12-01

Auroral radio emissions reveal physics of beam-plasma interactions and provide possibilities to remotely sense ionospheric plasma processes. Sato et al. [2012] recently discovered that auroral roar emissions, long known to occur at two and three times the electron gyrofrequency (fce), also occur at 4fce. Using data from wave receivers in the British Antarctic Survey Automatic Geophysical Observatories (BAS AGOs), we confirm the existence of 4fce-roars and observe for the first time 5fce-roars. A search at higher frequencies did not find higher harmonics, however. Both 4fce- and 5fce-roars only occur in sunlit conditions near the summer soltices. The harmonic emissions scale as expected with the strength of the geomagnetic field, and combining data from four stations with a wide range of magnetic field strengths suggests that the source height of the 4fce may lie around 245 km, significantly lower than the ˜ 275 km estimated for 2fce-roars. These observations show that the auroral roar generation mechanism acts under a broader set of plasma densities than previously considered, highlight how ubiquitous and robust the mechanism must be in different plasma environments, and suggest a broader application for remote sensing methods exploiting auroral roar, such as those described by Weatherwax et al. [2002]. References: Sato, Y., T. Ono, N. Sato, and Y. Ogawa, First observations of 4fce auroral roar emissions, Geophys. Res. Lett., 39, L07101, doi:10.1029/2012GL051205, 2012. Weatherwax, A.T., P.H. Yoon, and J. LaBelle, Model results and interpretation related to topside observations of auroral roar, J. Geophys. Res., 107, 10.1029/2001JA000315, 2002.

CA Air Resource Board's Off-Road Diesel Equipment Regulation 2017 DOORS Reporting.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jadhav, Pradnya

Sandia National Laboratories/CA (SNL/CA) operates 14 diesel vehicles subject to the Off-Road Diesel Equipment Regulation; all the vehicles are classified as low-use vehicles. Annually, SNL/CA must report to the CA Air Resources Board (ARB) the hour-meter readings on our Off-Road Low-Use Diesel Equipment. This is to verify to the CA ARB that our equipment is being operated less than 200 hr/yr and therefore is considered “low-use” and not subject to the strict emissions standards for off-road diesel equipment. The reporting is done using DOORS, the CA ARB’s on-line reporting tool. The hour meter data for CY2017 has already been submittedmore » using DOORS. The final step in the annual reporting process, which needs to be done by DOE, is to submit the Responsible Official Affirmation of Reporting (ROAR), which affirms that the information reported using DOORS is accurate. The ROAR is to be signed by a “Responsible Official” and can be submitted as a hardcopy ROAR or an electronic ROAR (e-ROAR) by March 1st, 2018. CA ARB’s preferred mechanism of ROAR submission is by using the e-ROAR.« less

78 FR 56650 - Boundary Description and Final Map for Roaring Wild and Scenic River, Mount Hood National Forest...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-13

... DEPARTMENT OF AGRICULTURE Forest Service Boundary Description and Final Map for Roaring Wild and... availability. SUMMARY: In accordance with section 3(b) of the Wild and Scenic Rivers Act, the USDA Forest Service, Washington Office, is transmitting the final boundary description and map of the Roaring Wild and...

33 CFR 100.914 - Trenton Rotary Roar on the River, Trenton, MI.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Trenton Rotary Roar on the River, Trenton, MI. 100.914 Section 100.914 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY REGATTAS AND MARINE PARADES SAFETY OF LIFE ON NAVIGABLE WATERS § 100.914 Trenton Rotary Roar on...

33 CFR 100.919 - International Bay City River Roar, Bay City, MI.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false International Bay City River Roar, Bay City, MI. 100.919 Section 100.919 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Bay City River Roar, Bay City, MI. (a) Regulated Area. A regulated area is established to include all...

33 CFR 100.919 - International Bay City River Roar, Bay City, MI.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false International Bay City River Roar, Bay City, MI. 100.919 Section 100.919 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Bay City River Roar, Bay City, MI. (a) Regulated Area. A regulated area is established to include all...

THE FORK AND THE KINASE: A DNA REPLICATION TALE FROM A CHK1 PERSPECTIVE

PubMed Central

González Besteiro, Marina A.; Gottifredi, Vanesa

2014-01-01

Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. The checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged-DNA. Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Such findings unveil a puzzling connection between Chk1 and DNA-lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, the multifaceted and versatile functions of Chk1 at ongoing forks and replication origins determine the extent and quality of the cellular response to replication stress. PMID:25795119

Floods in south-central Oklahoma and north-central Texas, October 1981

USGS Publications Warehouse

Buckner, Harold D.; Kurklin, Joanne K.

1984-01-01

Substantial reductions in peak stages and discharges on the West Fork Trinity River downstream from Eagle Mountain Reservoir were attained as a result of reservoir storage. All floodwater on the Elm Fork Trinity River was contained by reservoir storage thus preventing a potentially devastating flood downstream on the Trinity River. Maximum stages and discharges and/or contents were recorded during and after this major flood at 83 gaging stations, crest-stage stations, reservoir stations, and a miscellaneous site.

A nose that roars: anatomical specializations and behavioural features of rutting male saiga

PubMed Central

Frey, Roland; Volodin, Ilya; Volodina, Elena

2007-01-01

The involvement of the unique saiga nose in vocal production has been neglected so far. Rutting male saigas produce loud nasal roars. Prior to roaring, they tense and extend their noses in a highly stereotypic manner. This change of nose configuration includes dorsal folding and convex curving of the nasal vestibulum and is maintained until the roar ends. Red and fallow deer males that orally roar achieve a temporary increase of vocal tract length (vtl) by larynx retraction. Saiga males attain a similar effect by pulling their flexible nasal vestibulum rostrally, allowing for a temporary elongation of the nasal vocal tract by about 20%. Decrease of formant frequencies and formant dispersion, as acoustic effects of an increase of vtl, are assumed to convey important information on the quality of a dominant male to conspecifics, e.g. on body size and fighting ability. Nasal roaring in saiga may equally serve to deter rival males and to attract females. Anatomical constraints might have set a limit to the rostral pulling of the nasal vestibulum. It seems likely that the sexual dimorphism of the saiga nose was induced by sexual selection. Adult males of many mammalian species, after sniffing or licking female urine or genital secretions, raise their head and strongly retract their upper lip and small nasal vestibulum while inhalating orally. This flehmen behaviour is assumed to promote transport of non-volatile substances via the incisive ducts into the vomeronasal organs for pheromone detection. The flehmen aspect in saiga involves the extensive flexible walls of the greatly enlarged nasal vestibulum and is characterized by a distinctly concave configuration of the nose region, the reverse of that observed in nasal roaring. A step-by-step model for the gradual evolution of the saiga nose is presented here. PMID:17971116

The fork and the kinase: a DNA replication tale from a CHK1 perspective.

PubMed

González Besteiro, Marina A; Gottifredi, Vanesa

2015-01-01

Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. Checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged DNA. Subsequently, Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Indeed, such findings have unveiled a puzzling connection between Chk1 and DNA lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, Chk1 is a multifaceted and versatile signaling factor that acts at ongoing forks and replication origins to determine the extent and quality of the cellular response to replication stress. Copyright © 2014 Elsevier B.V. All rights reserved.

Ménière disease

MedlinePlus

... Ringing or roaring in the affected ear, called tinnitus Vertigo, or dizziness Severe vertigo is the symptom ... may have roaring or ringing in the ear (tinnitus), along with a sense of pressure in your ...

Spatial Variations In The Fate And Transport Of Metals In A Mining-Influenced Stream, North Fork Clear Creek, Colorado

EPA Science Inventory

North Fork Clear Creek (NFCC) receives acid-mine drainage (AMD) from multiple abandoned mines in the Clear Creek Watershed. Point sources of AMD originate In the Black Hawk/Central City region of the stream. Water chemistry also is influenced by several non-point sources of AMD,...

Sediment delivery in the North Fork of Caspar Creek

Treesearch

Raymond M. Rice

1996-01-01

Sediment delivery was estimated for 13 tributary watersheds and the North Fork of Caspar Creek. The ratio of sediment to erosion averaged 16.4%, ranging from 1.0% to 89.7%. Because the data were so highly skewed their median is a better indicator of central tendency than their mean. The median delivery ratio was 6.3%

Plant composition in oak savanna and woodland restoration at Prairie Fork Conservation Area in Missouri

Treesearch

Nadia E. Navarrete-Tindall; J.W. Van Sambeek; Jamie Coe; Warren Taylor

2007-01-01

The wooded areas of the Prairie Fork Conservation Area in central Missouri are typical of the oak/hickory forest/prairie transition zone that will require active management to restore pre-settlement, grass dominated savannas and open woodlands to improve habitat for wildlife. We initiated a management program to restore savannas and woodlands by reducing the midstory (...

The larynx of roaring and non-roaring cats.

PubMed

Hast, M H

1989-04-01

Dissections were made of the larynges of 14 species of the cat family, with representative specimens from all genera. It was found that the vocal folds of the larynx of genus Panthera (with the exception of the snow leopard) form the basic structure of a sound generator well-designed to produce a high acoustical energy. Combined with an efficient sound radiator (vocal tract) that can be adjusted in length, a Panthera can use its vocal instrument literally to blow its own horn with a 'roar'. Also, it is proposed that laryngeal morphology can be used as an anatomical character in mammalian taxonomy.

A dual role of BRCA1 in two distinct homologous recombination mediated repair in response to replication arrest

PubMed Central

Feng, Zhihui; Zhang, Junran

2012-01-01

Homologous recombination (HR) is a major mechanism utilized to repair blockage of DNA replication forks. Here, we report that a sister chromatid exchange (SCE) generated by crossover-associated HR efficiently occurs in response to replication fork stalling before any measurable DNA double-strand breaks (DSBs). Interestingly, SCE produced by replication fork collapse following DNA DSBs creation is specifically suppressed by ATR, a central regulator of the replication checkpoint. BRCA1 depletion leads to decreased RPA2 phosphorylation (RPA2-P) following replication fork stalling but has no obvious effect on RPA2-P following replication fork collapse. Importantly, we found that BRCA1 promotes RAD51 recruitment and SCE induced by replication fork stalling independent of ATR. In contrast, BRCA1 depletion leads to a more profound defect in RAD51 recruitment and SCE induced by replication fork collapse when ATR is depleted. We concluded that BRCA1 plays a dual role in two distinct HR-mediated repair upon replication fork stalling and collapse. Our data established a molecular basis for the observation that defective BRCA1 leads to a high sensitivity to agents that cause replication blocks without being associated with DSBs, and also implicate a novel mechanism by which loss of cell cycle checkpoints promotes BRCA1-associated tumorigenesis via enhancing HR defect resulting from BRCA1 deficiency. PMID:21954437

The Properties of Lion Roars and Electron Dynamics in Mirror Mode Waves Observed by the Magnetospheric MultiScale Mission

NASA Astrophysics Data System (ADS)

Breuillard, H.; Le Contel, O.; Chust, T.; Berthomier, M.; Retino, A.; Turner, D. L.; Nakamura, R.; Baumjohann, W.; Cozzani, G.; Catapano, F.; Alexandrova, A.; Mirioni, L.; Graham, D. B.; Argall, M. R.; Fischer, D.; Wilder, F. D.; Gershman, D. J.; Varsani, A.; Lindqvist, P.-A.; Khotyaintsev, Yu. V.; Marklund, G.; Ergun, R. E.; Goodrich, K. A.; Ahmadi, N.; Burch, J. L.; Torbert, R. B.; Needell, G.; Chutter, M.; Rau, D.; Dors, I.; Russell, C. T.; Magnes, W.; Strangeway, R. J.; Bromund, K. R.; Wei, H.; Plaschke, F.; Anderson, B. J.; Le, G.; Moore, T. E.; Giles, B. L.; Paterson, W. R.; Pollock, C. J.; Dorelli, J. C.; Avanov, L. A.; Saito, Y.; Lavraud, B.; Fuselier, S. A.; Mauk, B. H.; Cohen, I. J.; Fennell, J. F.

2018-01-01

Mirror mode waves are ubiquitous in the Earth's magnetosheath, in particular behind the quasi-perpendicular shock. Embedded in these nonlinear structures, intense lion roars are often observed. Lion roars are characterized by whistler wave packets at a frequency ˜100 Hz, which are thought to be generated in the magnetic field minima. In this study, we make use of the high time resolution instruments on board the Magnetospheric MultiScale mission to investigate these waves and the associated electron dynamics in the quasi-perpendicular magnetosheath on 22 January 2016. We show that despite a core electron parallel anisotropy, lion roars can be generated locally in the range 0.05-0.2fce by the perpendicular anisotropy of electrons in a particular energy range. We also show that intense lion roars can be observed up to higher frequencies due to the sharp nonlinear peaks of the signal, which appear as sharp spikes in the dynamic spectra. As a result, a high sampling rate is needed to estimate correctly their amplitude, and the latter might have been underestimated in previous studies using lower time resolution instruments. We also present for the first-time 3-D high time resolution electron velocity distribution functions in mirror modes. We demonstrate that the dynamics of electrons trapped in the mirror mode structures are consistent with the Kivelson and Southwood (1996) model. However, these electrons can also interact with the embedded lion roars: first signatures of electron quasi-linear pitch angle diffusion and possible signatures of nonlinear interaction with high-amplitude wave packets are presented. These processes can lead to electron untrapping from mirror modes.

77 FR 58799 - Radio Broadcasting Services; Roaring Springs, TX

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-24

...] Radio Broadcasting Services; Roaring Springs, TX AGENCY: Federal Communications Commission. ACTION... Subjects in 47 CFR Part 73 Radio, Radio broadcasting. Federal Communications Commission. Nazifa Sawez... Communications Commission proposes to amend 47 CFR part 73 as follows: PART 73--RADIO BROADCAST SERVICES 1. The...

Long-term survey of lion-roar emissions inside the terrestrial magnetosheath obtained from the STAFF-SA measurements onboard the Cluster spacecraft

NASA Astrophysics Data System (ADS)

Pisa, D.; Krupar, V.; Kruparova, O.; Santolik, O.

2017-12-01

Intense whistler-mode emissions known as 'lion-roars' are often observed inside the terrestrial magnetosheath, where the solar wind plasma flow slows down, and the local magnetic field increases ahead of a planetary magnetosphere. Plasma conditions in this transient region lead to the electron temperature anisotropy, which can result in the whistler-mode waves. The lion-roars are narrow-band emissions with typical frequencies between 0.1-0.5 Fce, where Fce is the electron cyclotron frequency. We present results of a long-term survey obtained by the Spatio Temporal Analysis Field Fluctuations - Spectral Analyzer (STAFF-SA) instruments on board the four Cluster spacecraft between 2001 and 2010. We have visually identified the time-frequency intervals with the intense lion-roar signature. Using the Singular Value Decomposition (SVD) method, we analyzed the wave propagation properties. We show the spatial, frequency and wave power distributions. Finally, the wave properties as a function of upstream solar wind conditions are discussed.

The larynx of roaring and non-roaring cats.

PubMed Central

Hast, M H

1989-01-01

Dissections were made of the larynges of 14 species of the cat family, with representative specimens from all genera. It was found that the vocal folds of the larynx of genus Panthera (with the exception of the snow leopard) form the basic structure of a sound generator well-designed to produce a high acoustical energy. Combined with an efficient sound radiator (vocal tract) that can be adjusted in length, a Panthera can use its vocal instrument literally to blow its own horn with a 'roar'. Also, it is proposed that laryngeal morphology can be used as an anatomical character in mammalian taxonomy. Images Fig. 1 PMID:2606766

78 FR 40402 - Radio Broadcasting Services; Roaring Springs, Texas

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-05

... FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 [MB Docket No. 12-236; RM-11671; DA 13-986] Radio Broadcasting Services; Roaring Springs, Texas AGENCY: Federal Communications Commission. ACTION: Final rule. SUMMARY: The Audio Division, at the request of Jesus B. Salazar, allots FM Channel 227A and deletes FM...

33 CFR 100.914 - Trenton Rotary Roar on the River, Trenton, MI.

Code of Federal Regulations, 2011 CFR

2011-07-01

... SECURITY REGATTAS AND MARINE PARADES SAFETY OF LIFE ON NAVIGABLE WATERS § 100.914 Trenton Rotary Roar on... on land located at position 42°7.7′ N; 083°10.7′ W, and along the shoreline to the point of origin...

Learning to Roar

ERIC Educational Resources Information Center

Cowhey, Mary

2009-01-01

In the author's school district, teachers and parents found a way to change the question. "How can their community support the grassroots leadership development and organizing efforts of low income parents and guardians to close the "opportunity gap"?" Together they learned to roar. In 2007, the author and her colleague created Families with…

DNA Replication Origins and Fork Progression at Mammalian Telomeres

PubMed Central

Higa, Mitsunori; Fujita, Masatoshi; Yoshida, Kazumasa

2017-01-01

Telomeres are essential chromosomal regions that prevent critical shortening of linear chromosomes and genomic instability in eukaryotic cells. The bulk of telomeric DNA is replicated by semi-conservative DNA replication in the same way as the rest of the genome. However, recent findings revealed that replication of telomeric repeats is a potential cause of chromosomal instability, because DNA replication through telomeres is challenged by the repetitive telomeric sequences and specific structures that hamper the replication fork. In this review, we summarize current understanding of the mechanisms by which telomeres are faithfully and safely replicated in mammalian cells. Various telomere-associated proteins ensure efficient telomere replication at different steps, such as licensing of replication origins, passage of replication forks, proper fork restart after replication stress, and dissolution of post-replicative structures. In particular, shelterin proteins have central roles in the control of telomere replication. Through physical interactions, accessory proteins are recruited to maintain telomere integrity during DNA replication. Dormant replication origins and/or homology-directed repair may rescue inappropriate fork stalling or collapse that can cause defects in telomere structure and functions. PMID:28350373

Millennial Generation Spirituality and Religion in the United States Army

DTIC Science & Technology

2013-04-01

their predecessor, Generation X, was raised in a postmodern culture , which makes their perspectives on life and the world particularly different than...respected internally and externally Consciousness Revolution against Institutions, Society Values Culture Wars and Roaring ‘90s Entering Young...Adulthood Consciousness Revolution against Institutions, Society Values Culture Wars and Roaring ‘90s 9-11, Global War on Terror

Taxonomy and phylogeny of black-and-white colobus monkeys. Inferences from an analysis of loud call variation.

PubMed

Oates, J F; Trocco, T F

1983-01-01

Field recordings of male loud calls (or roars) from each major form of black-and-white colobus monkey have been analyzed spectrographically, and features of tempo and pitch measured. Considered together with data on cranial dimensions, coat pattern, and geographical distribution, the results of this analysis suggest that there are five species of black-and-white colobus: Colobus angolensis, C. guereza, C. polykomos, C. satanas, and C. vellerosus. C. guereza and C. vellerosus may have differentiated most recently during a major arid event prior to the last Pleistocene glacial maximum; they have an identical low-pitched roar which we consider to be a shared, derived character. The other species, of which C. satanas has the most distinct roar, may belong to older lineages.

Recombinase and translesion DNA polymerase decrease the speed of replication fork progression during the DNA damage response in Escherichia coli cells

PubMed Central

Tan, Kang Wei; Pham, Tuan Minh; Furukohri, Asako; Maki, Hisaji; Akiyama, Masahiro Tatsumi

2015-01-01

The SOS response is a DNA damage response pathway that serves as a general safeguard of genome integrity in bacteria. Extensive studies of the SOS response in Escherichia coli have contributed to establishing the key concepts of cellular responses to DNA damage. However, how the SOS response impacts on the dynamics of DNA replication fork movement remains unknown. We found that inducing the SOS response decreases the mean speed of individual replication forks by 30–50% in E. coli cells, leading to a 20–30% reduction in overall DNA synthesis. dinB and recA belong to a group of genes that are upregulated during the SOS response, and encode the highly conserved proteins DinB (also known as DNA polymerase IV) and RecA, which, respectively, specializes in translesion DNA synthesis and functions as the central recombination protein. Both genes were independently responsible for the SOS-dependent slowdown of replication fork progression. Furthermore, fork speed was reduced when each gene was ectopically expressed in SOS-uninduced cells to the levels at which they are expressed in SOS-induced cells. These results clearly indicate that the increased expression of dinB and recA performs a novel role in restraining the progression of an unperturbed replication fork during the SOS response. PMID:25628359

Developing a Natural Gas-Powered Bus Rapid Transit Service. A Case Study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mitchell, George

2015-11-01

The Roaring Fork Transit Authority (RFTA) and its VelociRFTA Bus Rapid Transit (BRT) program are unique in many ways. For example, VelociRFTA was the first rural BRT system in the United States and the operational environment of the VelociRFTA BRT is one of the most severe in the country, with extreme winter temperatures and altitudes close to 8,000 feet. RFTA viewed high altitude operation as the most challenging characteristic when it began considering the use of natural gas. RFTA is the second-largest public transit system in Colorado behind Denver's Regional Transportation District (RTD), and it is one of the largestmore » rural public transit systems in the country. In 2013, RFTA accepted delivery of 22 new compressed natural gas (CNG) buses that went into service after completion of maintenance and refueling facilities earlier that year. This paper examines the lessons learned from RFTA's experience of investigating--and ultimately choosing--CNG for their new BRT program and focuses on the unique environment of RFTA's BRT application; the decision process to include CNG fueling in the project; unforeseen difficulties encountered in the operation of CNG buses; public perception; cost comparison to competing fuels; and considerations for indoor fueling facilities and project funding.« less

Developing a Natural Gas-Powered Bus Rapid Transit Service: A Case Study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mitchell, G.

2015-11-03

The Roaring Fork Transit Authority (RFTA) and its VelociRFTA Bus Rapid Transit (BRT) program are unique in many ways. For example, VelociRFTA was the first rural BRT system in the United States and the operational environment of the VelociRFTA BRT is one of the most severe in the country, with extreme winter temperatures and altitudes close to 8,000 feet. RFTA viewed high altitude operation as the most challenging characteristic when it began considering the use of natural gas. RFTA is the second-largest public transit system in Colorado behind Denver's Regional Transportation District (RTD), and it is one of the largestmore » rural public transit systems in the country. In 2013, RFTA accepted delivery of 22 new compressed natural gas (CNG) buses that went into service after completion of maintenance and refueling facilities earlier that year. This paper examines the lessons learned from RFTA's experience of investigating--and ultimately choosing--CNG for their new BRT program and focuses on the unique environment of RFTA's BRT application; the decision process to include CNG fueling in the project; unforeseen difficulties encountered in the operation of CNG buses; public perception; cost comparison to competing fuels; and considerations for indoor fueling facilities and project funding.« less

The ophiolitic North Fork terrane in the Salmon River region, central Klamath Mountains, California

USGS Publications Warehouse

Ando, C.J.; Irwin, W.P.; Jones, D.L.; Saleeby, J.B.

1983-01-01

The North Fork terrane is an assemblage of ophiolitic and other oceanic volcanic and sedimentary rocks that has been internally imbricated and folded. The ophiolitic rocks form a north-trending belt through the central part of the region and consist of a disrupted sequence of homogeneous gabbro, diabase, massive to pillowed basalt, and interleaved tectonitic harzburgite. U-Pb zircon age data on a plagiogranite pod from the gabbroic unit indicate that at least this part of the igneous sequence is late Paleozoic in age.The ophiolitic belt is flanked on either side by mafic volcanic and volcaniclastic rocks, limestone, bedded chert, and argillite. Most of the chert is Triassic, including much of Late Triassic age, but chert with uncertain stratigraphic relations at one locality is Permian. The strata flanking the east side of the ophiolitic belt face eastward, and depositional contacts between units are for the most part preserved. The strata on the west side of the ophiolitic belt are more highly disrupted than those on the east side, contain chert-argillite melange, and have unproven stratigraphic relation to either the ophiolitic rocks or the eastern strata.Rocks of the North Fork terrane do not show widespread evidence of penetrative deformation at elevated temperatures, except an early tectonitic fabric in the harzburgite. Slip-fiber foliation in serpentinite, phacoidal foliation in chert and mafic rocks, scaly foliation in argillite, and mesoscopic folds in bedded chert are consistent with an interpretation of large-scale anti-formal folding of the terrane about a north-south hinge found along the ophiolitic belt, but other structural interpretations are tenable. The age of folding of North Fork rocks is constrained by the involvement of Triassic and younger cherts and crosscutting Late Jurassic plutons. Deformation in the North Fork terrane must have spanned a short period of time because the terrane is bounded structurally above and below by Middle or Late Jurassic thrust faults.The North Fork terrane appears to contain no arc volcanic rocks or arc-derived detritus, suggesting that it neither constituted the base for an arc nor was in a basinal setting adjacent to an arc sediment source. Details of the progressive accretion and evolutionary relationship of the North Fork to other terranes of the Klamath Mountains are not yet clear.

Female harbor seal (Phoca vitulina) behavioral response to playbacks of underwater male acoustic advertisement displays

PubMed Central

Blades, Brittany; Parks, Susan E.

2018-01-01

During the breeding season, male harbor seals (Phoca vitulina) make underwater acoustic displays using vocalizations known as roars. These roars have been shown to function in territory establishment in some breeding areas and have been hypothesized to be important for female choice, but the function of these sounds remains unresolved. This study consisted of a series of playback experiments in which captive female harbor seals were exposed to recordings of male roars to determine if females respond to recordings of male vocalizations and whether or not they respond differently to roars from categories with different acoustic characteristics. The categories included roars with characteristics of dominant males (longest duration, lowest frequency), subordinate males (shortest duration, highest frequency), combinations of call parameters from dominant and subordinate males (long duration, high frequency and short duration, low frequency), and control playbacks of water noise and water noise with tonal signals in the same frequency range as male signals. Results indicate that overall females have a significantly higher level of response to playbacks that imitate male vocalizations when compared to control playbacks of water noise. Specifically, there was a higher level of response to playbacks representing dominant male vocalization when compared to the control playbacks. For most individuals, there was a greater response to playbacks representing dominant male vocalizations compared to playbacks representing subordinate male vocalizations; however, there was no statistical difference between those two playback types. Additionally, there was no difference between the playbacks of call parameter combinations and the controls. Investigating female preference for male harbor seal vocalizations is a critical step in understanding the harbor seal mating system and further studies expanding on this captive study will help shed light on this important issue. PMID:29607261

Female harbor seal (Phoca vitulina) behavioral response to playbacks of underwater male acoustic advertisement displays.

PubMed

Matthews, Leanna P; Blades, Brittany; Parks, Susan E

2018-01-01

During the breeding season, male harbor seals ( Phoca vitulina ) make underwater acoustic displays using vocalizations known as roars. These roars have been shown to function in territory establishment in some breeding areas and have been hypothesized to be important for female choice, but the function of these sounds remains unresolved. This study consisted of a series of playback experiments in which captive female harbor seals were exposed to recordings of male roars to determine if females respond to recordings of male vocalizations and whether or not they respond differently to roars from categories with different acoustic characteristics. The categories included roars with characteristics of dominant males (longest duration, lowest frequency), subordinate males (shortest duration, highest frequency), combinations of call parameters from dominant and subordinate males (long duration, high frequency and short duration, low frequency), and control playbacks of water noise and water noise with tonal signals in the same frequency range as male signals. Results indicate that overall females have a significantly higher level of response to playbacks that imitate male vocalizations when compared to control playbacks of water noise. Specifically, there was a higher level of response to playbacks representing dominant male vocalization when compared to the control playbacks. For most individuals, there was a greater response to playbacks representing dominant male vocalizations compared to playbacks representing subordinate male vocalizations; however, there was no statistical difference between those two playback types. Additionally, there was no difference between the playbacks of call parameter combinations and the controls. Investigating female preference for male harbor seal vocalizations is a critical step in understanding the harbor seal mating system and further studies expanding on this captive study will help shed light on this important issue.

The Late Cretaceous Middle Fork caldera, its resurgent intrusion, and enduring landscape stability in east-central Alaska

USGS Publications Warehouse

Bacon, Charles R.; Dusel-Bacon, Cynthia; Aleinikoff, John N.; Slack, John F.

2014-01-01

The Middle Fork is a relatively well preserved caldera within a broad region of Paleozoic metamorphic rocks and Mesozoic plutons bounded by northeast-trending faults. In the relatively downdropped and less deeply exhumed crustal blocks, Cretaceous–Early Tertiary silicic volcanic rocks attest to long-term stability of the landscape. Within the Middle Fork caldera, the granite porphyry is interpreted to have been exposed by erosion of thick intracaldera tuff from an asymmetric resurgent dome. The Middle Fork of the North Fork of the Fortymile River incised an arcuate valley into and around the caldera fill on the west and north and may have cut down from within an original caldera moat. The 70 Ma land surface is preserved beneath proximal outflow tuff at the west margin of the caldera structure and beneath welded outflow tuff 16–23 km east-southeast of the caldera in a paleovalley. Within ∼50 km of the Middle Fork caldera are 14 examples of Late Cretaceous (?)–Tertiary felsic volcanic and hypabyssal intrusive rocks that range in area from <1 km2 to ∼100 km2. Rhyolite dome clusters north and northwest of the caldera occupy tectonic basins associated with northeast-trending faults and are relatively little eroded. Lava of a latite complex, 12–19 km northeast of the caldera, apparently flowed into the paleovalley of the Middle Fork of the North Fork of the Fortymile River. To the northwest of the Middle Fork caldera, in the Mount Harper crustal block, mid-Cretaceous plutonic rocks are widely exposed, indicating greater total exhumation. To the southeast of the Middle Fork block, the Mount Veta block has been uplifted sufficiently to expose a ca. 68–66 Ma equigranular granitic pluton. Farther to the southeast, in the Kechumstuk block, the flat-lying outflow tuff remnant in Gold Creek and a regionally extensive high terrace indicate that the landscape there has been little modified since 70 Ma other than entrenchment of tributaries in response to post–2.7 Ma lowering of base level of the Yukon River associated with advance of the Cordilleran ice sheet.

On the generation of magnetosheath lion roars

NASA Technical Reports Server (NTRS)

Lee, L. C.; Wu, C. S.; Price, C. P.

1987-01-01

A theoretical model is proposed to discuss the electron dynamics associated with the mirror waves and their effects on the generation of the observed lion roars in the magnetosheath. It is pointed out that the usual double-adiabatic theory of hydromagnetics is not applicable to the electrons in mirror waves. Although the electron magnetic moment is conserved, the energy of each electron in the mirror waves is expected to be constant. Assuming an initial electron temperature anisotropy, it can be shown that in the low field region the electron temperature and thermal anisotropy are higher than the initial values, whereas in the high field region the electron temperature and anisotropy are lower. This point can lead to a theoretical explanation of the important features of the observed lion roars. Then present discussion complements the existing theories in the literature.

Vocal anatomy, tongue protrusion behaviour and the acoustics of rutting roars in free-ranging Iberian red deer stags (Cervus elaphus hispanicus)

PubMed Central

Frey, Roland; Volodin, Ilya; Volodina, Elena; Carranza, Juan; Torres-Porras, Jerónimo

2012-01-01

Roaring in rutting Iberian red deer stags Cervus elaphus hispanicus is unusual compared to other subspecies of red deer, which radiated from the Iberian refugium after the last glacial maximum. In all red deer stags, the larynx occupies a permanent low mid-neck resting position and is momentarily retracted almost down to the rostral end of the sternum during the production of rutting calls. Simultaneous with the retraction of the larynx, male Iberian red deer pronouncedly protrude the tongue during most of their rutting roars. This poses a mechanical challenge for the vocal tract (vt) and for the hyoid apparatus, as tongue and larynx are strongly pulled in opposite directions. This study (i) examines the vocal anatomy and the acoustics of the rutting roars in free-ranging male C. e. hispanicus; (ii) establishes a potential mechanism of simultaneous tongue protrusion and larynx retraction by applying a two-dimensional model based on graphic reconstructions in single video frames of unrestrained animals; and (iii) advances a hypothesis of evaporative cooling by tongue protrusion in the males of a subspecies of red deer constrained to perform all of the exhausting rutting activities, including acoustic display, in a hot and arid season. PMID:22257361

Oil and gas resources of the Cheat Mountain Further Planning Area (RARE II), Randolph County, West Virginia

USGS Publications Warehouse

Weed, E.G.

1981-01-01

Cheat Mountain Further Planning Area comprises about 7,720 acres in the Monongahela National Forest in east-central West Virginia, southeast of Elkins. The study area lies on a northeast-trending linear ridge bordered on the west by the Right Fork of Tygart River and on the east by Shavers Fork. It averages about 2 mi in length and 1½ mi in width. Altitudes on Cheat Mountain range from about 2,550 to 3,900 ft. 

Photolithography and Selective Etching of an Array of Quartz Tuning Fork Resonators with Improved Impact Resistance Characteristics

NASA Astrophysics Data System (ADS)

Lee, Sungkyu

2001-08-01

Quartz tuning fork blanks with improved impact-resistant characteristics for use in Qualcomm mobile station modem (MSM)-3000 central processing unit (CPU) chips for code division multiple access (CDMA), personal communication system (PCS), and global system for mobile communication (GSM) systems were designed using finite element method (FEM) analysis and suitable processing conditions were determined for the reproducible precision etching of a Z-cut quartz wafer into an array of tuning forks. Negative photoresist photolithography for the additive process was used in preference to positive photoresist photolithography for the subtractive process to etch the array of quartz tuning forks. The tuning fork pattern was transferred via a conventional photolithographical chromium/quartz glass template using a standard single-sided aligner and subsequent negative photoresist development. A tightly adhering and pinhole-free 600/2000 Å chromium/gold mask was coated over the developed photoresist pattern which was subsequently stripped in acetone. This procedure was repeated on the back surface of the wafer. With the protective metallization area of the tuning fork geometry thus formed, etching through the quartz wafer was performed at 80°C in a ± 1.5°C controlled bath containing a concentrated solution of ammonium bifluoride to remove the unwanted areas of the quartz wafer. The quality of the quartz wafer surface finish after quartz etching depended primarily on the surface finish of the quartz wafer prior to etching and the quality of quartz crystals used. Selective etching of a 100 μm quartz wafer could be achieved within 90 min at 80°C. A selective etching procedure with reproducible precision has thus been established and enables the photolithographic mass production of miniature tuning fork resonators.

Hydrogeologic framework of the North Fork and surrounding areas, Long Island, New York

USGS Publications Warehouse

Schubert, Christopher E.; Bova, Richard G.; Misut, Paul E.

2004-01-01

Ground water on the North Fork of Long Island is the sole source of drinking water, but the supply is vulnerable to saltwater intrusion and upconing in response to heavy pumping. Information on the area's hydrogeologic framework is needed to analyze the effects of pumping and drought on ground-water levels and the position of the freshwater-saltwater interface. This will enable water-resource managers and water-supply purveyors to evaluate a wide range of water-supply scenarios to safely meet water-use demands. The extent and thickness of hydrogeologic units and position of the freshwater-saltwater interface were interpreted from previous work and from exploratory drilling during this study.The fresh ground-water reservoir on the North Fork consists of four principal freshwater flow systems (referred to as Long Island mainland, Cutchogue, Greenport, and Orient) within a sequence of unconsolidated Pleistocene and Late Cretaceous deposits. A thick glacial-lake-clay unit appears to truncate underlying deposits in three buried valleys beneath the northern shore of the North Fork. Similar glacial-lake deposits beneath eastern and east-central Long Island Sound previously were inferred to be younger than the surficial glacial deposits exposed along the northern shore of Long Island. Close similarities in thickness and upper-surface altitude between the glacial-lake-clay unit on the North Fork and the glacial-lake deposits in Long Island Sound indicate, however, that the two are correlated at least along the North Fork shore.The Matawan Group and Magothy Formation, undifferentiated, is the uppermost Cretaceous unit on the North Fork and constitutes the Magothy aquifer. The upper surface of this unit contains a series of prominent erosional features that can be traced beneath Long Island Sound and the North Fork. Northwest-trending buried ridges extend several miles offshore from areas southeast of Rocky Point and Horton Point. A promontory in the irregular, north-facing cuesta slope extends offshore from an area southwest of Mattituck Creek and James Creek. Buried valleys that trend generally southeastward beneath Long Island Sound extend onshore northeast of Hashamomuck Pond and east of Goldsmith Inlet.An undifferentiated Pleistocene confining layer, the lower confining unit, consists of apparently contiguous units of glacial-lake, marine, and nonmarine clay. This unit is more than 200 feet thick in buried valleys filled with glacial-lake clay along the northern shore, but elsewhere on the North Fork, it is generally less than 50 feet thick and presumably represents an erosional remnant of marine clay. Its upper surface is generally 75 feet or more below sea level where it overlies buried valleys, and is generally 100 feet or less below sea level in areas where marine clay has been identified.A younger unit of glacial-lake deposits, the upper confining unit, is a local confining layer and underlies a sequence of late Pleistocene moraine and outwash deposits. This unit is thickest (more than 45 feet thick) beneath two lowland areas--near Mattituck Creek and James Creek, and near Hashamomuck Pond--but pinches out close to the northern and southern shores and is locally absent in inland areas of the North Fork. Its upper-surface altitude generally rises to near sea level toward the southern shore.Freshwater in the Orient flow system is limited to the upper glacial aquifer above the top of the lower confining unit. The upper confining unit substantially impedes the downward flow of freshwater in inland parts of the Greenport flow system. Deep freshwater within the lower confining unit in the east-central part of the Cutchogue flow system probably is residual from an interval of lower sea level. The upper confining unit is absent or only a few feet thick in the west-central part of the Cutchogue flow system and does not substantially impede the downward flow of freshwater, but the lower confining unit probably impedes the downward flow of freshwater within a southeast-trending buried valley in this area.

On the generation of magnetosheath lion roars

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, L.C.; Wu, C.S.; Price, C.P.

1987-03-01

A theoretical model is proposed to discuss the electron dynamics associated with the mirror waves and their effects on the generation of the observed lion roars in the magnetosheath. It is pointed out that the usual double-adiabatic theory of hydromagnetics is not applicable to the electrons in mirror waves. Although the electron magnetic moment is conserved, the energy of each electron in the mirror waves is expected to be constant (because of the high electron speed along the magnetic field). Assuming an initial electron temperature anisotropy, the authors can show that in the low field region the electron temperature andmore » thermal anisotropy are higher than the initial values, whereas in the high field region the electron temperature and anisotropy are lower. This point can lead to a theoretical explanation of the important features of the observed lion roars. The present discussion complements the existing theories in the literature.« less

First observation of lion roar-like emissions in Saturn's magnetosheath

NASA Astrophysics Data System (ADS)

Pisa, David; Sulaiman, Ali H.; Santolik, Ondrej; Hospodarsky, George B.; Kurth, William S.; Gurnett, Donald A.

2017-04-01

Electromagnetic whistler mode waves known as "lion roars" have been reported by many missions inside the terrestrial magnetosheath. We show the observation of similar intense emissions in Saturn's magnetosheath as detected by the Cassini spacecraft. The emissions were observed inside the dawn sector (MLT˜0730) of the magnetosheath over a time period of nine hours before the satellite crossed the bow shock and entered the solar wind. The emissions were narrow-banded with a typical frequency of about 15 Hz well below the local electron cyclotron frequency (fce ˜100 Hz). Using the minimum variance analysis method, we show that the waves are right hand circularly polarized and propagate at small wave normal angles with respect to the ambient magnetic field. Here, for the first time, we report the evidence of lion roar-like emissions in Saturn's magnetosheath which represents a new and unique parameter regime.

Advancing the Fork detector for quantitative spent nuclear fuel verification

DOE PAGES

Vaccaro, S.; Gauld, I. C.; Hu, J.; ...

2018-01-31

The Fork detector is widely used by the safeguards inspectorate of the European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) to verify spent nuclear fuel. Fork measurements are routinely performed for safeguards prior to dry storage cask loading. Additionally, spent fuel verification will be required at the facilities where encapsulation is performed for acceptance in the final repositories planned in Sweden and Finland. The use of the Fork detector as a quantitative instrument has not been prevalent due to the complexity of correlating the measured neutron and gamma ray signals with fuel inventories and operator declarations.more » A spent fuel data analysis module based on the ORIGEN burnup code was recently implemented to provide automated real-time analysis of Fork detector data. This module allows quantitative predictions of expected neutron count rates and gamma units as measured by the Fork detectors using safeguards declarations and available reactor operating data. This study describes field testing of the Fork data analysis module using data acquired from 339 assemblies measured during routine dry cask loading inspection campaigns in Europe. Assemblies include both uranium oxide and mixed-oxide fuel assemblies. More recent measurements of 50 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel are also analyzed. An evaluation of uncertainties in the Fork measurement data is performed to quantify the ability of the data analysis module to verify operator declarations and to develop quantitative go/no-go criteria for safeguards verification measurements during cask loading or encapsulation operations. The goal of this approach is to provide safeguards inspectors with reliable real-time data analysis tools to rapidly identify discrepancies in operator declarations and to detect potential partial defects in spent fuel assemblies with improved reliability and minimal false positive alarms. Finally, the results are summarized, and sources and magnitudes of uncertainties are identified, and the impact of analysis uncertainties on the ability to confirm operator declarations is quantified.« less

Advancing the Fork detector for quantitative spent nuclear fuel verification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vaccaro, S.; Gauld, I. C.; Hu, J.

The Fork detector is widely used by the safeguards inspectorate of the European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) to verify spent nuclear fuel. Fork measurements are routinely performed for safeguards prior to dry storage cask loading. Additionally, spent fuel verification will be required at the facilities where encapsulation is performed for acceptance in the final repositories planned in Sweden and Finland. The use of the Fork detector as a quantitative instrument has not been prevalent due to the complexity of correlating the measured neutron and gamma ray signals with fuel inventories and operator declarations.more » A spent fuel data analysis module based on the ORIGEN burnup code was recently implemented to provide automated real-time analysis of Fork detector data. This module allows quantitative predictions of expected neutron count rates and gamma units as measured by the Fork detectors using safeguards declarations and available reactor operating data. This study describes field testing of the Fork data analysis module using data acquired from 339 assemblies measured during routine dry cask loading inspection campaigns in Europe. Assemblies include both uranium oxide and mixed-oxide fuel assemblies. More recent measurements of 50 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel are also analyzed. An evaluation of uncertainties in the Fork measurement data is performed to quantify the ability of the data analysis module to verify operator declarations and to develop quantitative go/no-go criteria for safeguards verification measurements during cask loading or encapsulation operations. The goal of this approach is to provide safeguards inspectors with reliable real-time data analysis tools to rapidly identify discrepancies in operator declarations and to detect potential partial defects in spent fuel assemblies with improved reliability and minimal false positive alarms. Finally, the results are summarized, and sources and magnitudes of uncertainties are identified, and the impact of analysis uncertainties on the ability to confirm operator declarations is quantified.« less

Advancing the Fork detector for quantitative spent nuclear fuel verification

NASA Astrophysics Data System (ADS)

Vaccaro, S.; Gauld, I. C.; Hu, J.; De Baere, P.; Peterson, J.; Schwalbach, P.; Smejkal, A.; Tomanin, A.; Sjöland, A.; Tobin, S.; Wiarda, D.

2018-04-01

The Fork detector is widely used by the safeguards inspectorate of the European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) to verify spent nuclear fuel. Fork measurements are routinely performed for safeguards prior to dry storage cask loading. Additionally, spent fuel verification will be required at the facilities where encapsulation is performed for acceptance in the final repositories planned in Sweden and Finland. The use of the Fork detector as a quantitative instrument has not been prevalent due to the complexity of correlating the measured neutron and gamma ray signals with fuel inventories and operator declarations. A spent fuel data analysis module based on the ORIGEN burnup code was recently implemented to provide automated real-time analysis of Fork detector data. This module allows quantitative predictions of expected neutron count rates and gamma units as measured by the Fork detectors using safeguards declarations and available reactor operating data. This paper describes field testing of the Fork data analysis module using data acquired from 339 assemblies measured during routine dry cask loading inspection campaigns in Europe. Assemblies include both uranium oxide and mixed-oxide fuel assemblies. More recent measurements of 50 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel are also analyzed. An evaluation of uncertainties in the Fork measurement data is performed to quantify the ability of the data analysis module to verify operator declarations and to develop quantitative go/no-go criteria for safeguards verification measurements during cask loading or encapsulation operations. The goal of this approach is to provide safeguards inspectors with reliable real-time data analysis tools to rapidly identify discrepancies in operator declarations and to detect potential partial defects in spent fuel assemblies with improved reliability and minimal false positive alarms. The results are summarized, and sources and magnitudes of uncertainties are identified, and the impact of analysis uncertainties on the ability to confirm operator declarations is quantified.

Vocal anatomy, tongue protrusion behaviour and the acoustics of rutting roars in free-ranging Iberian red deer stags (Cervus elaphus hispanicus).

PubMed

Frey, Roland; Volodin, Ilya; Volodina, Elena; Carranza, Juan; Torres-Porras, Jerónimo

2012-03-01

Roaring in rutting Iberian red deer stags Cervus elaphus hispanicus is unusual compared to other subspecies of red deer, which radiated from the Iberian refugium after the last glacial maximum. In all red deer stags, the larynx occupies a permanent low mid-neck resting position and is momentarily retracted almost down to the rostral end of the sternum during the production of rutting calls. Simultaneous with the retraction of the larynx, male Iberian red deer pronouncedly protrude the tongue during most of their rutting roars. This poses a mechanical challenge for the vocal tract (vt) and for the hyoid apparatus, as tongue and larynx are strongly pulled in opposite directions. This study (i) examines the vocal anatomy and the acoustics of the rutting roars in free-ranging male C. e. hispanicus; (ii) establishes a potential mechanism of simultaneous tongue protrusion and larynx retraction by applying a two-dimensional model based on graphic reconstructions in single video frames of unrestrained animals; and (iii) advances a hypothesis of evaporative cooling by tongue protrusion in the males of a subspecies of red deer constrained to perform all of the exhausting rutting activities, including acoustic display, in a hot and arid season. © 2012 The Authors. Journal of Anatomy © 2012 Anatomical Society.

Environmental settings of the South Fork Iowa River basin, Iowa, and the Bogue Phalia basin, Mississippi, 2006-10

USGS Publications Warehouse

McCarthy, Kathleen A.; Rose, Claire E.; Kalkhoff, Stephen J.

2012-01-01

Studies of the transport and fate of agricultural chemicals in different environmental settings were conducted by the U.S. Geological Survey (USGS) National Water-Quality Assessment (NAWQA) Program's Agricultural Chemicals Team (ACT) at seven sites across the Nation, including the South Fork Iowa River basin in central Iowa and the Bogue Phalia basin in northwestern Mississippi. The South Fork Iowa River basin is representative of midwestern agriculture, where corn and soybeans are the predominant crops and a large percentage of the cultivated land is underlain by artificial drainage. The Bogue Phalia basin is representative of corn, soybean, cotton, and rice cropping in the humid, subtropical southeastern United States. Details of the environmental settings of these basins and the data-collection activities conducted by the USGS ACT over the 2006-10 study period are described in this report.

An Archeological Survey of the Cross Ditch No.2 and Central Ditch Cleanout Project Poinsett County, Arkansas

DTIC Science & Technology

1986-10-01

REPORT October 1986 Report prepared by 0 % -’• Rt. 1 Box 186 ,o, West Fork, AR 72774 000 w ua0’ (501) 839-3663 0fr Memphis District, Corps of Engineers...Cleanout Project Poisett Co., AR c DACW66-86-M-1097 6. AUTHORS) Robert A. Taylor Carol S. Spears 7. PERFORW6NG ORGAN.ZATiON ANtMES) AND AD.JE.S3ýES) 8...ý-KiGkMING ORGANiAT.,NR•EPORT NUMEER Spears Professional Environmental & Archaeological Researcl Service West Fork, AR 9. SPONSORING MCNTO7’.G AG

Assessing RUSLE and hill-slope soil movement modeling in the central Appalachians

Treesearch

Jingxin Wang; Pam Edwards; Greg W. Hamons; William Goff

2010-01-01

The determination of the topographical attributes responsible for the origination and transfer of sediment were investigated in a central Appalachian mixed hardwood forest from 2002 through 2005. Two study watersheds were chosen on the left fork of Clover Run within the Indian Run watershed in Tucker County, West Virginia. Silt fence was installed around all the stream...

A reconnaissance of the effects of a forest fire on water quality in Kings Canyon National Park, California

USGS Publications Warehouse

Hoffman, Ray J.; Ferreira, Rodger F.

1976-01-01

Following two forest fires in the Roaring River drainage basin, Kings Canyon National Park, Calif., water samples were collected from May to July 1974 to determine water-quality changes resulting from the fires. Field measurements included alkalinity , pH, specific conductance, temperature, and discharge. Samples were analyzed in the laboratory for major dissolved chemical constituents, selected plant nutrients, trace metals, suspended sediment, total organic carbon, and seston. Periphytic algae and benthic invertebrate samples were collected. A noticeable increase in the concentration of nitrogen was found in Roaring River immediately downstream from the Moraine Creek fire. The increase in the concentration of inorganic nitrogen compounds, however, was not great enough to pose a serious threat to the aquatic ecosystem. High total organic nitrogen concentrations may have been due, in part, to factors other than the effect of fire. The results of other water-quality measurements were typical of dilute Sierra Nevada streams and indicate that Roaring River was not adversely affected by the fires. (Woodard-USGS)

Far-travelled permian chert of the North Fork terrane, Klamath mountains, California

USGS Publications Warehouse

Mankinen, E.A.; Irwin, W.P.; Blome, C.D.

1996-01-01

Permian chert in the North Fork terrane and correlative rocks of the Klamath Mountains province has a remanent magnetization that is prefolding and presumably primary. Paleomagnetic results indicate that the chert formed at a paleolatitude of 8.6?? ?? 2.5?? but in which hemisphere remains uncertain. This finding requires that these rocks have undergone at least 8.6?? ?? 4.4?? of northward transport relative to Permian North America since their deposition. Paleontological evidence suggests that the Permian limestone of the Eastern Klamath terrane originated thousands of kilometers distant from North America. The limestone of the North Fork terrane may have formed at a similar or even greater distance as suggested by its faunal affinity to the Eastern Klamath terrane and more westerly position. Available evidence indicates that convergence of the North Fork and composite Central Metamorphic-Eastern Klamath terranes occurred during Triassic or Early Jurassic time and that their joining together was a Middle Jurassic event. Primary and secondary magnetizations indicate that the new composite terrane containing these and other rocks of the Western Paleozoic and Triassic belt behaved as a single rigid block that has been latitudinally concordant with the North American craton since Middle Jurassic time.

Demographics and density estimates of two three-toed box turtle (Terrapene carolina triunguis) populations within forest and restored prairie sites in central Missouri.

PubMed

O'Connor, Kelly M; Rittenhouse, Chadwick D; Millspaugh, Joshua J; Rittenhouse, Tracy A G

2015-01-01

Box turtles (Terrapene carolina) are widely distributed but vulnerable to population decline across their range. Using distance sampling, morphometric data, and an index of carapace damage, we surveyed three-toed box turtles (Terrapene carolina triunguis) at 2 sites in central Missouri, and compared differences in detection probabilities when transects were walked by one or two observers. Our estimated turtle densities within forested cover was less at the Thomas S. Baskett Wildlife Research and Education Center, a site dominated by eastern hardwood forest (d = 1.85 turtles/ha, 95% CI [1.13, 3.03]) than at the Prairie Fork Conservation Area, a site containing a mix of open field and hardwood forest (d = 4.14 turtles/ha, 95% CI [1.99, 8.62]). Turtles at Baskett were significantly older and larger than turtles at Prairie Fork. Damage to the carapace did not differ significantly between the 2 populations despite the more prevalent habitat management including mowing and prescribed fire at Prairie Fork. We achieved improved estimates of density using two rather than one observer at Prairie Fork, but negligible differences in density estimates between the two methods at Baskett. Error associated with probability of detection decreased at both sites with the addition of a second observer. We provide demographic data on three-toed box turtles that suggest the use of a range of habitat conditions by three-toed box turtles. This case study suggests that habitat management practices and their impacts on habitat composition may be a cause of the differences observed in our focal populations of turtles.

Geomorphic Change Induced by 100 years of Flow Alteration on the Diamond Fork River, Central Utah

NASA Astrophysics Data System (ADS)

Jones, J.; Belmont, P.; Wilcock, P. R.

2017-12-01

Changes in hydrology and sediment supply affect the form of rivers. The rate of change of fluvial form is controlled by a variety of factors, including valley confinement, sediment size, and antecedent condition. The Diamond Fork River in central Utah has been altered by trans-basin flows delivered from the Colorado River system for over a century. Beginning in 1915, water used for irrigation was delivered through a tributary, Sixth Water Creek, with daily summer flows regularly exceeding the 50 - 100 year flood. Elevated flows caused drastic geomorphic change - resulting in incision and widening of the channel, and the destruction of riparian vegetation. Beginning in 1997, the outlet for the trans-basin diversion was moved downstream on Sixth Water, bypassing a large landslide, and flows were drastically reduced in 2004 through management actions. We delineated eight distinct process domains for the Sixth Water-Diamond Fork system and examined the response of each process domain to the altered flow and sediment regimes through the analysis of aerial photographs and repeat cross-sections. We measured a variety of channel metrics, including channel width, areal extent of bars and islands, and sinuosity in ArcGIS. Results indicate that unconfined reaches that were wide and braided during the period of elevated flows have narrowed to become single threaded and meandering in response to the reduced flows. Confined reaches have experienced minor changes since the reduction in flows, suggesting that confinement is a primary control on the degree of channel response. These findings and complimentary studies will provide managers of Sixth Water and Diamond Fork with a greater understanding of the physical response of the streams, and the resulting effects on ecological communities.

Lion roars and nonoscillatory drift mirror waves in the magnetosheath

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tsurutani, B.T.; Smith, E.J.; Anderson, R.R.

1982-08-01

A complete set of ISEE plasma wave, plasma, and field data are used to identify the plasma instability responsible for the generation of extremely low frequency (ELF) electromagnetic lion roars. Lion roars detected close to the magnetopause are generated by the cyclotron instability of anisotropic (T/sup -//sub perpendicular//T/sup -//sub parallel/approx. =1.2) thermal electrons when the local plasma critical energy, E/sub M/ = B/sup 2//8..pi..N, falls to values (E/sub M/ approx.10--30 eV) close to or below the electron thermal energy, 25 eV, as a result of decreases in B. The lion roars are terminated by increases in the ambient magnetic fieldmore » magnitude and consequential increases in E/sub M/ to values greater than 100 eV. Because there are few resonant particles at these high energies, the growth rate decreases by 3 orders of magnitude and measurable growth ceases. The value of the absolute upper limit of the frequency of unstable waves predicted by theory, ..omega../sub max/ = A/sup -/..cap omega../sup -//(A/sup -/+1), is compared with observations. The predictions and observations are found to be in general, but not exact, agreement. Several possible explanations are explored. The quasi-periodic, approx.20-s magnetic and plasma oscillations which cause the variations in E/sub M/ and hence alternately drive the cyclotron waves unstable and then stable are also investigated.« less

Lower Paleozoic deep-water facies of the Medfra area, central Alaska: A section in Geologic studies in Alaska by the U.S. Geological Survey, 1997

USGS Publications Warehouse

Dumoulin, Julie A.; Bradley, Dwight C.; Harris, Anita G.; Repetski, John E.

1999-01-01

Deep-water facies, chiefly hemipelagic deposits and turbidites, of Cambrian through Devonian age are widely exposed in the Medfra and Mt. McKinley quadrangles. These strata include the upper part of the Telsitna Formation (Middle-Upper Ordovician) and the Paradise Fork Formation (Lower Silurian-Lower Devonian) in the Nixon Fork terrane, the East Fork Hills Formation (Upper Cambrian-Lower Devonian) in the East Fork subterrane of the Minchumina terrane, and the chert and argillite unit (Ordovician) and the argillite and quartzite unit (Silurian- Devonian? and possibly older) in the Telida subterrane of the Minchumina terrane.In the western part of the study area (Medfra quadrangle), both hemipelagic deposits and turbidites are largely calcareous and were derived from the Nixon Fork carbonate platform. East- ern exposures (Mt. McKinley quadrangle; eastern part of the Telida subterrane) contain much less carbonate; hemipelagic strata are mostly chert, and turbidites contain abundant rounded quartz and lesser plagioclase and potassium feldspar. Deep-water facies in the Medfra quadrangle correlate well with rocks of the Dillinger terrane exposed to the south (McGrath quadrangle), but coeval strata in the Mt. McKinley quadrangle are compositionally similar to rocks to the northeast (Livengood quadrangle). Petrographic data thus suggest that the Telida subterranes presently defined is an artificial construct made up of two distinct sequences of disparate provenance.Restoration of 90 and 150 km of dextral strike-slip on the Iditarod and Farewell faults, respectively, aligns the deep-water strata of the Minchumina and Dillinger terranes in a position east of the Nixon Fork carbonate platform. This restoration supports the interpretation that lower Paleozoic rocks in the Nixon Fork and Dillinger terranes, and in the western part of the Minchumina terrane (East Fork subterrane and western part of the Telida subterrane), formed along a single continental margin. Rocks in the eastern part of the Telida subterrane are compositionally distinct from those to the west and may have had a different origin and history.

"Hunting with a Knife and... Fork": Examining Central Coherence in Autism, Attention Deficit/Hyperactivity Disorder, and Typical Development with a Linguistic Task

ERIC Educational Resources Information Center

Booth, Rhonda; Happe, Francesca

2010-01-01

A local processing bias, referred to as "weak central coherence," has been postulated to underlie key aspects of autism spectrum disorder (ASD). Little research has examined whether individual differences in this cognitive style can be found in typical development, independent of intelligence, and how local processing relates to executive control.…

Recovery from the DNA Replication Checkpoint

PubMed Central

Chaudhury, Indrajit; Koepp, Deanna M.

2016-01-01

Checkpoint recovery is integral to a successful checkpoint response. Checkpoint pathways monitor progress during cell division so that in the event of an error, the checkpoint is activated to block the cell cycle and activate repair pathways. Intrinsic to this process is that once repair has been achieved, the checkpoint signaling pathway is inactivated and cell cycle progression resumes. We use the term “checkpoint recovery” to describe the pathways responsible for the inactivation of checkpoint signaling and cell cycle re-entry after the initial stress has been alleviated. The DNA replication or S-phase checkpoint monitors the integrity of DNA synthesis. When replication stress is encountered, replication forks are stalled, and the checkpoint signaling pathway is activated. Central to recovery from the S-phase checkpoint is the restart of stalled replication forks. If checkpoint recovery fails, stalled forks may become unstable and lead to DNA breaks or unusual DNA structures that are difficult to resolve, causing genomic instability. Alternatively, if cell cycle resumption mechanisms become uncoupled from checkpoint inactivation, cells with under-replicated DNA might proceed through the cell cycle, also diminishing genomic stability. In this review, we discuss the molecular mechanisms that contribute to inactivation of the S-phase checkpoint signaling pathway and the restart of replication forks during recovery from replication stress. PMID:27801838

Floods of September 15-16, 1992, in the Thompson, Weldon, and Chariton River basins, south-central Iowa

USGS Publications Warehouse

Eash, D.A.; Koppensteiner, B.A.

1997-01-01

Water-surface-elevation profiles and peak discharges for the floods of September 15-16, 1992, in the Thompson, Weldon, and Chariton River Basins, south-central Iowa, are presented in this report. The profiles illustrate the 1992 floods along the Thompson, Weldon, Chariton, and South Fork Chariton Rivers and along Elk Creek in the south-central Iowa counties of Adair, Clarke, Decatur, Lucas, Madison, Ringgold, Union, and Wayne. Water-surface-elevation profiles for the floods of July 4, 1981, along the Chariton River in Lucas County and along the South Fork Chariton River in Wayne County also are included in the report for comparative purposes. The September 15-16, 1992, floods are the largest known peak discharges at gaging stations Thompson River at Davis City (station number 06898000) 57,000 cubic feet per second, Weldon River near Leon (station number 06898400) 76,200 cubic feet per second, Chariton River near Chariton (station number 06903400) 37,700 cubic feet per second, and South Fork Chariton River near Promise City (station number 06903700) 70,600 cubic feet per second. The peak discharges were, respectively, 1.7, 2.6, 1.4, and 2.1 times larger than calculated 100-year recurrence-interval discharges. The report provides information on flood stages and discharges and floodflow frequencies for streamflow-gaging stations in the Thompson, Weldon, and Chariton River Basins using flood information collected through 1995. Information on temporary bench marks and reference points established in the Thompson and Weldon River Basins during 1994-95, and in the Chariton River Basin during 1983-84 and 1994-95, also is included in the report. A flood history summarizes rainfall conditions and damages for floods that occurred during 1947, 1959, 1981, 1992, and 1993.

Space Shuttle Main Engine Public Test Firing

NASA Image and Video Library

2000-07-25

A new NASA Space Shuttle Main Engine (SSME) roars to the approval of more than 2,000 people who came to John C. Stennis Space Center in Hancock County, Miss., on July 25 for a flight-certification test of the SSME Block II configuration. The engine, a new and significantly upgraded shuttle engine, was delivered to NASA's Kennedy Space Center in Florida for use on future shuttle missions. Spectators were able to experience the 'shake, rattle and roar' of the engine, which ran for 520 seconds - the length of time it takes a shuttle to reach orbit.

Baseline geochemical data for stream sediment and surface water samples from Panther Creek, the Middle Fork of the Salmon River, and the Main Salmon River from North Fork to Corn Creek, collected prior to the severe wildfires of 2000 in central Idaho

USGS Publications Warehouse

Eppinger, Robert G.; Briggs, Paul H.; Brown, Zoe Ann; Crock, James G.; Meier, Allen; Theodorakos, Peter M.; Wilson, Stephen A.

2001-01-01

In 1996, the U.S. Geological Survey conducted a reconnaissance baseline geochemical study in central Idaho. The purpose of the baseline study was to establish a 'geochemical snapshot' of the area, as a datum for monitoring future change in the geochemical landscape, whether natural or human-induced. This report presents the methology, analytical results, and sample descriptions for water, sediment, and heavy-mineral concentrate samples collected during this geochemical investigation. In the summer of 2000, the Clear Creek, Little Pistol, and Shellrock wildfires swept across much of the area that was sampled. Thus, these data represent a pre-fire baseline geochemical dataset. A 2001 post- fire study is planned and will involve re-sampling of the pre-fire baseline sites, to allow for pre- and post-fire comparison.

24. A SECTION OF OPEN BALUSTRADE AND AN INTERMEDIATE PEDESTAL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. A SECTION OF OPEN BALUSTRADE AND AN INTERMEDIATE PEDESTAL (CENTERED ON A PYLON) OVER THE CENTRAL PORTIONS OF THE BRIDGE. THE WALKS ARE CONCRETE, WITH AN INTEGRAL CURB. THE ROADWAY IS ASPHALT. - Main Street Bridge, Spanning East Fork Whitewater River, Richmond, Wayne County, IN

The mouse that roared: neural mechanisms of social hierarchy.

PubMed

Wang, Fei; Kessels, Helmut W; Hu, Hailan

2014-11-01

Hierarchical social status greatly influences behavior and health. Human and animal studies have begun to identify the brain regions that are activated during the formation of social hierarchies. They point towards the prefrontal cortex (PFC) as a central regulator, with brain areas upstream of the PFC conveying information about social status, and downstream brain regions executing dominance behavior. This review summarizes our current knowledge on the neural circuits that control social status. We discuss how the neural mechanisms for various types of dominance behavior can be studied in laboratory rodents by selective manipulation of neuronal activity or synaptic plasticity. These studies may help in finding the cause of social stress-related mental and physical health problems. Copyright © 2014 Elsevier Ltd. All rights reserved.

Geologic map of the Jam Up Cave and Pine Crest quadrangles, Shannon, Texas, and Howell Counties, Missouri

USGS Publications Warehouse

Weary, David J.; Orndorff, Randall C.; Repetski, John E.

2013-01-01

The Jam Up Cave and Pine Crest 7.5-minute quadrangles are located in south-central Missouri within the Salem Plateau region of the Ozark Plateaus physiographic province. About 2,400 to 3,100 feet (ft) of flat-lying to gently dipping Lower Paleozoic sedimentary rocks, mostly dolomite, chert, sandstone, and orthoquartzite, overlie Mesoproterozoic igneous basement rocks. Unconsolidated residuum, colluvium, terrace deposits, and alluvium overlie the sedimentary rocks. Numerous karst features, such as sinkholes, caves, and springs, have formed in the carbonate rocks. Many streams are spring fed. The topography is a dissected karst plain with elevations ranging from about 690 ft where the Jacks Fork River exits the northeastern corner of the Jam Up Cave quadrangle to about 1,350 ft in upland areas along the north-central edge and southwestern corner of the Pine Crest quadrangle. The most prominent physiographic feature is the valley of the Jacks Fork River. This reach of the upper Jacks Fork, with its clean, swiftly-flowing water confined by low cliffs and bluffs, provides one of the most beautiful canoe float trips in the nation. Most of the land in the quadrangles is privately owned and used primarily for grazing cattle and horses and growing timber. A large minority of the land within the quadrangles is publicly owned by the Ozark National Scenic Riverways of the National Park Service. Geologic mapping for this investigation was conducted in 2005 and 2006.

Model documentation for relations between continuous real-time and discrete water-quality constituents in the North Fork Ninnescah River upstream from Cheney Reservoir, south-central Kansas, 1999--2009

USGS Publications Warehouse

Stone, Mandy L.; Graham, Jennifer L.; Gatotho, Jackline W.

2013-01-01

Cheney Reservoir in south-central Kansas is one of the primary sources of water for the city of Wichita. The North Fork Ninnescah River is the largest contributing tributary to Cheney Reservoir. The U.S. Geological Survey has operated a continuous real-time water-quality monitoring station since 1998 on the North Fork Ninnescah River. Continuously measured water-quality physical properties include streamflow, specific conductance, pH, water temperature, dissolved oxygen, and turbidity. Discrete water-quality samples were collected during 1999 through 2009 and analyzed for sediment, nutrients, bacteria, and other water-quality constituents. Regression models were developed to establish relations between discretely sampled constituent concentrations and continuously measured physical properties to estimate concentrations of those constituents of interest that are not easily measured in real time because of limitations in sensor technology and fiscal constraints. Regression models were published in 2006 that were based on a different dataset collected during 1997 through 2003. This report updates those models using discrete and continuous data collected during January 1999 through December 2009. Models also were developed for five new constituents, including additional nutrient species and indicator bacteria. The water-quality information in this report is important to the city of Wichita because it allows the concentrations of many potential pollutants of interest, including nutrients and sediment, to be estimated in real time and characterized over conditions and time scales that would not be possible otherwise.

River mainstem thermal regimes influence population structuring within an Appalachian brook trout population

USGS Publications Warehouse

Aunins, Aaron W.; Petty, J. Todd; King, Timothy L.; Schilz, Mariya; Mazik, Patricia M.

2015-01-01

Brook trout (Salvelinus fontinalis) often exist as highly differentiated populations, even at small spatial scales, due either to natural or anthropogenic sources of isolation and low rates of dispersal. In this study, we used molecular approaches to describe the unique population structure of brook trout inhabiting the Shavers Fork watershed, located in eastern West Virginia, and contrast it to nearby populations in tributaries of the upper Greenbrier River and North Fork South Branch Potomac Rivers. Bayesian and maximum likelihood clustering methods identified minimal population structuring among 14 collections of brook trout from throughout the mainstem and tributaries of Shavers Fork, highlighting the role of the cold-water mainstem for connectivity and high rates of effective migration among tributaries. In contrast, the Potomac and Greenbrier River collections displayed distinct levels of population differentiation among tributaries, presumably resulting from tributary isolation by warm-water mainstems. Our results highlight the importance of protecting and restoring cold-water mainstem habitats as part of region-wide brook trout conservation efforts. In addition, our results from Shavers Fork provide a contrast to previous genetic studies that characterize Appalachian brook trout as fragmented isolates rather than well-mixed populations. Additional study is needed to determine whether the existence of brook trout as genetically similar populations among tributaries is truly unique and whether connectivity among brook trout populations can potentially be restored within other central Appalachian watersheds.

VASQUEZ PEAK WILDERNESS STUDY AREA, AND ST. LOUIS PEAK, AND WILLIAMS FORK ROADLESS AREAS, COLORADO.

USGS Publications Warehouse

Theobald, P.K.; Bielski, A.M.

1984-01-01

A mineral-resource survey was conducted during the years 1979-82 in the Vasquez Peak Wilderness Study Area and in the St. Louis Peak and Williams Fork Roadless Areas, central Front Range, Colorado. Probable resource potential for the occurrence of copper, lead, zinc, and silver in massive sulfide deposits has been identified in calcareous metamorphic rocks in the northern part of the St. Louis Peak Roadless Area and in the southern part of the Williams Fork Roadless Area. A probable resource potential for vein-type uranium deposits is identified along the Berthoud Pass fault zone in the eastern part of the Vasquez Peak Wilderness Study Area. A large area encompassing the eastern and southeastern part of each of the three areas has probable and substantiated potential for either high-grade lead-zinc-silver vein deposits, or larger, lower-grade clustered vein deposits. A probable resource potential for stockwork molybdenum deposits related to porphyry molybdenum type mineralization exists beneath the lead-zinc-silver-rich veins. The nature of the geologic terrane indicates little likelihood for the occurrence of organic fuels.

Mercury and selenium concentrations in biofilm, macroinvertebrates, and fish collected in the Yankee Fork of the Salmon River, Idaho, USA, and their potential effects on fish health.

PubMed

Rhea, Darren T; Farag, Aïda M; Harper, David D; McConnell, Elizabeth; Brumbaugh, William G

2013-01-01

The Yankee Fork is a large tributary of the Salmon River located in central Idaho, USA, with an extensive history of placer and dredge-mining activities. Concentrations of selenium (Se) and mercury (Hg) in various aquatic trophic levels were measured in the Yankee Fork during 2001 and 2002. Various measurements of fish health were also performed. Sites included four on the mainstem of the Yankee Fork and two off-channel sites in partially reclaimed dredge pools used as rearing habitat for cultured salmonid eggs and fry. Hg concentrations in whole mountain whitefish and shorthead sculpin ranged from 0.28 to 0.56 μg/g dry weight (dw), concentrations that are generally less than those reported to have significant impacts on fish. Biofilm and invertebrates ranged from 0.05 to 0.43 μg Hg/g dw. Se concentrations measured in biota samples from the Yankee Fork were greater than many representative samples collected in the Snake and Columbia watersheds and often exceeded literature-based toxic thresholds. Biofilm and invertebrates ranged from 0.58 to 4.66 μg Se/g dw. Whole fish ranged from 3.92 to 7.10 μg Se/g dw, and gonads ranged from 6.91 to 31.84 μg Se/g dw. Whole-body Se concentrations exceeded reported toxicological thresholds at three of four sites and concentrations in liver samples were mostly greater than concentrations shown to have negative impacts on fish health. Histological examinations performed during this study noted liver abnormalities, especially in shorthead sculpin, a bottom-dwelling species.

Mercury and selenium concentrations in biofilm, macroinvertebrates, and fish collected in the Yankee Fork of the Salmon River, Idaho, USA, and their potential effects on fish health

USGS Publications Warehouse

Rhea, Darren T.; Farag, Aïda M.; Harper, David D.; McConnell, Elizabeth; Brumbaugh, William G.

2013-01-01

The Yankee Fork is a large tributary of the Salmon River located in central Idaho, USA, with an extensive history of placer and dredge-mining activities. Concentrations of selenium (Se) and mercury (Hg) in various aquatic trophic levels were measured in the Yankee Fork during 2001 and 2002. Various measurements of fish health were also performed. Sites included four on the mainstem of the Yankee Fork and two off-channel sites in partially reclaimed dredge pools used as rearing habitat for cultured salmonid eggs and fry. Hg concentrations in whole mountain whitefish and shorthead sculpin ranged from 0.28 to 0.56 μg/g dry weight (dw), concentrations that are generally less than those reported to have significant impacts on fish. Biofilm and invertebrates ranged from 0.05 to 0.43 μg Hg/g dw. Se concentrations measured in biota samples from the Yankee Fork were greater than many representative samples collected in the Snake and Columbia watersheds and often exceeded literature-based toxic thresholds. Biofilm and invertebrates ranged from 0.58 to 4.66 μg Se/g dw. Whole fish ranged from 3.92 to 7.10 μg Se/g dw, and gonads ranged from 6.91 to 31.84 μg Se/g dw. Whole-body Se concentrations exceeded reported toxicological thresholds at three of four sites and concentrations in liver samples were mostly greater than concentrations shown to have negative impacts on fish health. Histological examinations performed during this study noted liver abnormalities, especially in shorthead sculpin, a bottom-dwelling species.

Occurrence and distribution of fecal indicator bacteria, and physical and chemical indicators of water quality in streams receiving discharge from Dallas/Fort Worth International Airport and vicinity, North-Central Texas, 2008

USGS Publications Warehouse

Harwell, Glenn R.; Mobley, Craig A.

2009-01-01

This report, done by the U.S. Geological Survey in cooperation with Dallas/Fort Worth International (DFW) Airport in 2008, describes the occurrence and distribution of fecal indicator bacteria (fecal coliform and Escherichia [E.] coli), and the physical and chemical indicators of water quality (relative to Texas Surface Water Quality Standards), in streams receiving discharge from DFW Airport and vicinity. At sampling sites in the lower West Fork Trinity River watershed during low-flow conditions, geometric mean E. coli counts for five of the eight West Fork Trinity River watershed sampling sites exceeded the Texas Commission on Environmental Quality E. coli criterion, thus not fully supporting contact recreation. Two of the five sites with geometric means that exceeded the contact recreation criterion are airport discharge sites, which here means that the major fraction of discharge at those sites is from DFW Airport. At sampling sites in the Elm Fork Trinity River watershed during low-flow conditions, geometric mean E. coli counts exceeded the geometric mean contact recreation criterion for seven (four airport, three non-airport) of 13 sampling sites. Under low-flow conditions in the lower West Fork Trinity River watershed, E. coli counts for airport discharge sites were significantly different from (lower than) E. coli counts for non-airport sites. Under low-flow conditions in the Elm Fork Trinity River watershed, there was no significant difference between E. coli counts for airport sites and non-airport sites. During stormflow conditions, fecal indicator bacteria counts at the most downstream (integrator) sites in each watershed were considerably higher than counts at those two sites during low-flow conditions. When stormflow sample counts are included with low-flow sample counts to compute a geometric mean for each site, classification changes from fully supporting to not fully supporting contact recreation on the basis of the geometric mean contact recreation criterion. All water temperature measurements at sampling sites in the lower West Fork Trinity River watershed were less than the maximum criterion for water temperature for the lower West Fork Trinity segment. Of the measurements at sampling sites in the Elm Fork Trinity River watershed, 95 percent were less than the maximum criterion for water temperature for the Elm Fork Trinity River segment. All dissolved oxygen concentrations were greater than the minimum criterion for stream segments classified as exceptional aquatic life use. Nearly all pH measurements were within the pH criterion range for the classified segments in both watersheds, except for those at one airport site. For sampling sites in the lower West Fork Trinity River watershed, all annual average dissolved solids concentrations were less than the maximum criterion for the lower West Fork Trinity segment. For sampling sites in the Elm Fork Trinity River, nine of the 13 sites (six airport, three non-airport) had annual averages that exceeded the maximum criterion for that segment. For ammonia, 23 samples from 12 different sites had concentrations that exceeded the screening level for ammonia. Of these 12 sites, only one non-airport site had more than the required number of exceedances to indicate a screening level concern. Stormflow total suspended solids concentrations were significantly higher than low-flow concentrations at the two integrator sites. For sampling sites in the lower West Fork Trinity River watershed, all annual average chloride concentrations were less than the maximum annual average chloride concentration criterion for that segment. For the 13 sampling sites in the Elm Fork Trinity River watershed, one non-airport site had an annual average concentration that exceeded the maximum annual average chloride concentration criterion for that segment.

Replication fork reversal triggers fork degradation in BRCA2-defective cells.

PubMed

Mijic, Sofija; Zellweger, Ralph; Chappidi, Nagaraja; Berti, Matteo; Jacobs, Kurt; Mutreja, Karun; Ursich, Sebastian; Ray Chaudhuri, Arnab; Nussenzweig, Andre; Janscak, Pavel; Lopes, Massimo

2017-10-16

Besides its role in homologous recombination, the tumor suppressor BRCA2 protects stalled replication forks from nucleolytic degradation. Defective fork stability contributes to chemotherapeutic sensitivity of BRCA2-defective tumors by yet-elusive mechanisms. Using DNA fiber spreading and direct visualization of replication intermediates, we report that reversed replication forks are entry points for fork degradation in BRCA2-defective cells. Besides MRE11 and PTIP, we show that RAD52 promotes stalled fork degradation and chromosomal breakage in BRCA2-defective cells. Inactivation of these factors restores reversed fork frequency and chromosome integrity in BRCA2-defective cells. Conversely, impairing fork reversal prevents fork degradation, but increases chromosomal breakage, uncoupling fork protection, and chromosome stability. We propose that BRCA2 is dispensable for RAD51-mediated fork reversal, but assembles stable RAD51 nucleofilaments on regressed arms, to protect them from degradation. Our data uncover the physiopathological relevance of fork reversal and illuminate a complex interplay of homologous recombination factors in fork remodeling and stability.BRCA2 is involved in both homologous recombination (HR) and the protection of stalled replication forks from degradation. Here the authors reveal how HR factors cooperate in fork remodeling, showing that BRCA2 supports RAD51 loading on the regressed arms of reversed replication forks to protect them from degradation.

77 FR 50668 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-22

... County, Texas, and Incorporated Areas. Specifically, it addresses the flooding sources: Black Fork Creek, Black Fork Creek Tributary BF-1, Black Fork Creek Tributary BF-M-1, Black Fork Creek Tributary D, Black Fork Creek Tributary D-1, Black Fork Creek Tributary D-2, Black Fork Creek Tributary D-3, Butler Creek...

Water-quality assessment of Lakes Maumelle and Winona, Arkansas, 1991 through 2003

USGS Publications Warehouse

Galloway, Joel M.; Green, W. Reed

2004-01-01

Lakes Maumelle and Winona are water-supply reservoirs for the Little Rock and North Little Rock metropolitan areas in central Arkansas. In addition to water supply, the reservoirs are used for recreation and fish and wildlife habitat. The purpose of this report is to describe the hydrology and water quality of Lakes Maumelle and Winona and their inflows from data collected by the U.S. Geological Survey in cooperation with Central Arkansas Water for calendar years 1991 through 2003. The main inflows into Lakes Maumelle and Winona, the Maumelle River and Alum Fork Saline River, exhibited typical seasonal variability in streamflow with high flows usually occurring in the late fall, winter, and early spring, and low or no flow in the summer and early fall. The highest annual mean streamflow occurred in 1991 and the lowest annual mean streamflow occurred in 1992 for the Maumelle River and 1995 for the Alum Fork Saline River. Water quality measured in Lakes Maumelle and Winona varied spatially and temporally. Although total phosphorus concentrations were substantially higher at the upper ends of the lakes than at the lower ends of the lakes, nitrogen and orthophosphorus concentrations were not significantly different among the sampling sites on each lake. The highest concentrations of nitrogen generally were measured in 1991 and from 1998 through 2003 at all of the sampling sites. The highest total phosphorus concentrations were measured from 1994 to 1996 and from 1998 to 2001 on Lake Maumelle and from 1993 to 1994 on Lake Winona. Total and dissolved organic carbon concentrations were similar among sites on each lake and the greatest concentrations were measured in 1996 and 1997 at all of the sites. The chlorophyll a concentrations varied seasonally, with the highest concentrations in October and November, but were relatively uniform spatially and annually in Lakes Maumelle and Winona for 1991 through 2003. Water clarity was greater at the lower ends of the lakes than at the upper ends. Secchi depth varied seasonally, with the greatest depth from July to September and the least depth during October through December. There was no apparent trend in Secchi depth over the entire sampling period. The trophic state indices calculated from near-surface concentrations of total phosphorus and chlorophyll a for Lakes Maumelle and Winona indicated that they generally were oligotrophic although they fluctuated in time between mesotrophic and oligotrophic conditions. Water-quality concentrations generally were less for the main inflow to Lake Winona, the Alum Fork Saline River, than for the Maumelle River, Bringle, Yount, and Reece Creeks, which flow into Lake Maumelle. Nutrient concentrations for the Maumelle and Alum Fork Saline Rivers remained fairly uniform from 1991 through 2003. Suspended-sediment concentrations generally were greatest at Bringle Creek. Concentrations of fecal streptococci measured at the Alum Fork Saline River were similar to concentrations measured at the Maumelle River, and fecal coliforms concentrations for the Alum Fork Saline River were approximately half the concentration measured at the Maumelle River. Bringle and Reece Creeks had greater concentrations of fecal coliforms and fecal streptococci than the Maumelle River, and Yount Creek had the lowest concentration of fecal streptococci among all the sites. Annual loads of nutrients, dissolved organic carbon, and suspended sediment estimated for the Maumelle River and the Alum Fork Saline River were similar between sites and varied with time from 1991 through 2003. Annual loads were greatest in 1991 for the Maumelle and Alum Fork Saline Rivers and the least in 2000 for the Maumelle River and 1995 for the Alum Fork Saline River. Estimated loads also demonstrated seasonal trends with the highest daily loads in the winter and fall and lowest daily loads in the summer for both sites. Annual yields of nutrients and dissolved organic carbon computed

3. View of Clark Fork Vehicle Bridge facing southwest. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. View of Clark Fork Vehicle Bridge facing southwest. Bridge from north shore of Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Microhabitat use by brook trout inhabiting small tributaries and a large river main stem: Implications for stream habitat restoration in the central Appalachians

USGS Publications Warehouse

Hansbarger, Jeff L.; Petty, J. Todd; Mazik, Patricia M.

2008-01-01

Brook trout (Salvelinus fontinalis) habitat restoration is needed across a range of stream sizes; however, studies quantifying brook trout habitat preferences in streams of differing sizes are rare. We used radio-telemetry to quantify adult brook trout microhabitat use in a central Appalachian watershed, the upper Shavers Fork of the Cheat River in eastern West Virginia. Our objectives were to: 1) quantify non-random microhabitat use by adult brook trout in the Shavers Fork main stem (drainage area = 32 km2) and an adjacent tributary, Rocky Run (drainage area = 7 km2); and 2) construct stream-specific habitat suitability curves (HSCs) for four important microhabitat variables (depth, average current velocity, maximum current velocity within one meter, and distance to cover). Brook trout used a subset of available microhabitats in both the main stem and Rocky Run: trout tended to occupy microhabitats that were deeper, higher velocity, and closer to cover than expected by chance alone. Although specific microhabitat values differed between the main stem and tributary populations, the overall patterns in brook trout microhabitat use were consistent regardless of stream size. Habitat suitability curves were constructed based on brook trout microhabitat use and will be used to design and monitor the effectiveness of future habitat restoration efforts in the Shavers Fork watershed. Our results suggest that habitat enhancement projects that increase the availability of deep, high velocity microhabitats adjacent to cover would benefit brook trout in both small tributaries and larger river main stems.

4. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shoreof Clark Fork River showing 4 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

First Observation of Lion Roar Emission in Saturn's Magnetosheath

NASA Astrophysics Data System (ADS)

Píša, D.; Sulaiman, A. H.; Santolík, O.; Hospodarsky, G. B.; Kurth, W. S.; Gurnett, D. A.

2018-01-01

We present an observation of intense emissions in Saturn's magnetosheath as detected by the Cassini spacecraft. The emissions are observed in the dawn sector (magnetic local time ˜06:45) of the magnetosheath over a time period of 11 h before the spacecraft crossed the bow shock and entered the unshocked solar wind. They are found to be narrow-banded with a peak frequency of about 0.16 fce, where fce is the local electron gyrofrequency. Using plane wave propagation analysis, we show that the waves are right hand circularly polarized in the spacecraft frame and propagate at small wave normal angles (<10∘) with respect to the ambient magnetic field. Electromagnetic waves with the same properties known as "lion roars" have been reported by numerous missions in the terrestrial magnetosheath. Here we show the first evidence such emission outside the terrestrial environment. Our observations suggest that lion roars are a solar-system-wide phenomenon and capable of existing in a broad range of parameter space. This also includes 1 order of magnitude difference in frequencies. We anticipate our result to provide new insight into such emissions in a new parameter regime characterized by a higher plasma beta (owing to the substantially higher Mach number bow shock) compared to Earth.

2. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

7. View of Clark Fork Vehicle Bridge facing northwest. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. View of Clark Fork Vehicle Bridge facing northwest. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

1. View of Clark Fork Vehicle Bridge facing west. Panorama ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. View of Clark Fork Vehicle Bridge facing west. Panorama showing the entire span of bridge from north shore of the Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Observation of hectometric auroral radio emissions in Iceland

NASA Astrophysics Data System (ADS)

Sato, Y.; Ono, T.; Iizima, M.; Sato, N.

2006-12-01

The Earth's auroral region is an active radio source at frequencies from a few hertz to several megahertz. In the hectometric range, it was found that Terrestrial Hectometric Radiation (THR) is related to auroras by observations of the Ohzora satellite [Oya et al.(1985)]. In resent research, Shinbori et al. [2003] showed that occurrence of THR follows SC by several minutes using the Akebono satellite data. On the ground, auroral roar and MF burst were discovered by Kellogg and Monson [1979, 1984] and Weatherwax et al. [1994] in the northern Canada, respectively. Because there is not enough physical and geophysical characterization of these radio emissions, the physical mechanism of these phenomena in the auroral ionosphere has not been fully understood yet. We set up new observation system at Husafell station in Iceland in September, 2005 and have started to observe auroral radio emissions. Radio signals, which are received by the cross loop antennas, are converted into left- and right- handed polarized components within the frequency range from 1 MHz to 5 MHz. Based on the calibration of system, it was found that the possibility of occurence would be smaller than expected due to the low sensitivity because average power spectrum densities of auroral roar and MF burst are 50-100 nV/m/Hz^1/2. So, the system was planed to be upgraded in this September, which makes it possible to detect auroral roar and MF burst. It is expected that the detail physical process will be elucidated by clarifying the spectrum, polarization, dependence on the geomagnetic activity, and so on. In this presentation, we will show the improved points of the new system and preliminary observation results. There is a basic question whether auroral roar and MF burst observed on the ground are generated by the same process as THR observed by satellites. By comparing the results from the ground-based observation and the Akebono satellite observation of THR, it becomes possible to obtain a new picture of auroral radio emissions.

5. View of Clark Fork Vehicle Bridge facing east. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. View of Clark Fork Vehicle Bridge facing east. Bridge from south shore of Clark Fork River-southernmost span. 1900-era Northern Pacific Railway Bridge in background. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

8. View of Clark Fork Vehicle Bridge facing southwest. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. View of Clark Fork Vehicle Bridge facing southwest. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

20. View of Clark Fork Vehicle Bridge facing up. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. View of Clark Fork Vehicle Bridge facing up. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

18. View of Clark Fork Vehicle Bridge facing north. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

18. View of Clark Fork Vehicle Bridge facing north. Looking at north concrete abutment and timber stringers. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

19. View of Clark Fork Vehicle Bridge facing north. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. View of Clark Fork Vehicle Bridge facing north. Looking at north abutment and underside of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

22. View of Clark Fork Vehicle Bridge facing downwest side. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. View of Clark Fork Vehicle Bridge facing down-west side. Looking at road deck and vertical laced channel. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

21. View of Clark Fork Vehicle Bridge facing west. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

21. View of Clark Fork Vehicle Bridge facing west. Looking at bridge deck, guard rail, juncture of two bridge spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost span. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Influence of tectonic folding on rockfall susceptibility, American Fork Canyon, Utah, USA

USGS Publications Warehouse

Coe, J.A.; Harp, E.L.

2007-01-01

We examine rockfall susceptibility of folded strata in the Sevier fold-thrust belt exposed in American Fork Canyon in north-central Utah. Large-scale geologic mapping, talus production data, rock-mass-quality measurements, and historical rockfall data indicate that rockfall susceptibility is correlated with limb dip and curvature of the folded, cliff-forming Mississippian limestones. On fold limbs, rockfall susceptibility increases as dip increases. This relation is controlled by several factors, including an increase in adverse dip conditions and apertures of discontinuities, and shearing by flexural slip during folding that has reduced the friction angles of discontinuities by smoothing surface asperities. Susceptibility is greater in fold hinge zones than on adjacent limbs primarily because there are greater numbers of discontinuities in hinge zones. We speculate that susceptibility increases in hinge zones as fold curvature becomes tighter.

Rad53 regulates replication fork restart after DNA damage in Saccharomyces cerevisiae

PubMed Central

Szyjka, Shawn J.; Aparicio, Jennifer G.; Viggiani, Christopher J.; Knott, Simon; Xu, Weihong; Tavaré, Simon; Aparicio, Oscar M.

2008-01-01

Replication fork stalling at a DNA lesion generates a damage signal that activates the Rad53 kinase, which plays a vital role in survival by stabilizing stalled replication forks. However, evidence that Rad53 directly modulates the activity of replication forks has been lacking, and the nature of fork stabilization has remained unclear. Recently, cells lacking the Psy2–Pph3 phosphatase were shown to be defective in dephosphorylation of Rad53 as well as replication fork restart after DNA damage, suggesting a mechanistic link between Rad53 deactivation and fork restart. To test this possibility we examined the progression of replication forks in methyl-methanesulfonate (MMS)-damaged cells, under different conditions of Rad53 activity. Hyperactivity of Rad53 in pph3Δ cells slows fork progression in MMS, whereas deactivation of Rad53, through expression of dominant-negative Rad53-KD, is sufficient to allow fork restart during recovery. Furthermore, combined deletion of PPH3 and PTC2, a second, unrelated Rad53 phosphatase, results in complete replication fork arrest and lethality in MMS, demonstrating that Rad53 deactivation is a key mechanism controlling fork restart. We propose a model for regulation of replication fork progression through damaged DNA involving a cycle of Rad53 activation and deactivation that coordinates replication restart with DNA repair. PMID:18628397

23. View of Clark Fork Vehicle Bridge facing upwest side. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. View of Clark Fork Vehicle Bridge facing up-west side. Looking at structural connection of top chord, vertical laced channel and diagonal bars. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

13. View of Clark Fork Vehicle Bridge facing south. Concrete ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. View of Clark Fork Vehicle Bridge facing south. Concrete barrier blocks access. Plaque was originally located where strioed traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

12. View of Clark Fork Vehicle Bridge facing south. Approach ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. View of Clark Fork Vehicle Bridge facing south. Approach from the north road. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Tinnitus

MedlinePlus

Tinnitus is often described as a ringing in the ears. It also can sound like roaring, clicking, ... one or both ears. Millions of Americans have tinnitus. People with severe tinnitus may have trouble hearing, ...

Two new species of Pterostichus Bonelli subgenus Pseudoferonina Ball (Coleoptera, Carabidae, Pterostichini) from the mountains of central Idaho, U.S.A.

PubMed

Bergdahl, James C; Kavanaugh, David H

2011-01-01

Two new species of Pterostichus Bonelli subgenus Pseudoferonina Ball, are described from the mountains of central Idaho: Pterostichus bousqueti Bergdahl [type locality = small tributaries of South Fork of Payette River watershed, ca. 1170 m (3840 ft), 44.0675°; -115.6822°, near Lowman, Salmon River Mountains, Boise County, Idaho, U.S.A.] and Pterostichus lolo Bergdahl [type locality = Cottonwood/Orogrande Creek, ca. 870 m (2850 ft), 46.5528°; -115.5522°, North Fork of Clearwater River watershed, Clearwater Mountains, near Bungalow, Clearwater County, Idaho, U.S.A.]. Males of Pterostichus bousqueti and Pterostichus lolo are easily distinguished from each other and the seven previously described Pseudoferonina species by the form of the median lobe of the aedeagus, and from most individuals of the other species of Pseudoferonina in Idaho by features of pronotal shape and macrosculpture. Both species appear to be obligate ripicolous hygrophiles, restricted in distribution primarily to the margins of small montane streams in forested areas. Widespread intensive stream surveys for Pseudoferonina over many years indicate the geographic ranges of both species are highly localized, and additional undescribed species may occur in Idaho.

Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability.

PubMed

Schalbetter, Stephanie A; Mansoubi, Sahar; Chambers, Anna L; Downs, Jessica A; Baxter, Jonathan

2015-08-18

Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA precatenation formed. Although fork rotation predominates at replication termination, in vitro studies have suggested that it also occurs frequently during elongation. However, the factors that influence fork rotation and how rotation and precatenation may influence other replication-associated processes are unknown. Here we analyze the causes and consequences of fork rotation in budding yeast. We find that fork rotation and precatenation preferentially occur in contexts that inhibit topoisomerase action ahead of the fork, including stable protein-DNA fragile sites and termination. However, generally, fork rotation and precatenation are actively inhibited by Timeless/Tof1 and Tipin/Csm3. In the absence of Tof1/Timeless, excessive fork rotation and precatenation cause extensive DNA damage following DNA replication. With Tof1, damage related to precatenation is focused on the fragile protein-DNA sites where fork rotation is induced. We conclude that although fork rotation and precatenation facilitate unwinding in hard-to-replicate contexts, they intrinsically disrupt normal chromosome duplication and are therefore restricted by Timeless/Tipin.

14. View of Clark Fork Vehicle Bridge facing north. Approach ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. View of Clark Fork Vehicle Bridge facing north. Approach from the south. Concrete barrier blocks access. Plaque was originally located where striped traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

Meniere's Disease

MedlinePlus

... dizziness, a roaring sound in your ears called tinnitus, hearing loss that comes and goes and the ... on suddenly or after a short period of tinnitus or muffled hearing. Some people have single attacks ...

Ear Disorders

MedlinePlus

... most common illness in infants and young children. Tinnitus, a roaring in your ears, can be the ... problems in your inner ear; its symptoms include tinnitus and dizziness. Ear barotrauma is an injury to ...

Meniere's Disease

MedlinePlus

... vertigo (attacks of a spinning sensation), hearing loss, tinnitus (a roaring, buzzing, or ringing sound in the ... of the disease, hearing loss often becomes permanent. Tinnitus and fullness of the ear may come and ...

Making the Words Roar

ERIC Educational Resources Information Center

Armstrong, Thomas

2004-01-01

Every student is directly enabled to experience the full majesty and power of the literacy lion. Strategies for multiple intelligences that can help generate reading strategies specifically tailored to students are presented.

Juno Captures the Roar of Jupiter

NASA Image and Video Library

2016-06-30

NASA's Juno spacecraft has crossed into Jupiter's immense magnetic field. Juno's Waves instrument recorded the crossing of the bow shock on June 24, 2016, represented by the following animation and sound.

Endonuclease EEPD1 Is a Gatekeeper for Repair of Stressed Replication Forks*

PubMed Central

Kim, Hyun-Suk; Nickoloff, Jac A.; Wu, Yuehan; Williamson, Elizabeth A.; Sidhu, Gurjit Singh; Reinert, Brian L.; Jaiswal, Aruna S.; Srinivasan, Gayathri; Patel, Bhavita; Kong, Kimi; Burma, Sandeep; Lee, Suk-Hee; Hromas, Robert A.

2017-01-01

Replication is not as continuous as once thought, with DNA damage frequently stalling replication forks. Aberrant repair of stressed replication forks can result in cell death or genome instability and resulting transformation to malignancy. Stressed replication forks are most commonly repaired via homologous recombination (HR), which begins with 5′ end resection, mediated by exonuclease complexes, one of which contains Exo1. However, Exo1 requires free 5′-DNA ends upon which to act, and these are not commonly present in non-reversed stalled replication forks. To generate a free 5′ end, stalled replication forks must therefore be cleaved. Although several candidate endonucleases have been implicated in cleavage of stalled replication forks to permit end resection, the identity of such an endonuclease remains elusive. Here we show that the 5′-endonuclease EEPD1 cleaves replication forks at the junction between the lagging parental strand and the unreplicated DNA parental double strands. This cleavage creates the structure that Exo1 requires for 5′ end resection and HR initiation. We observed that EEPD1 and Exo1 interact constitutively, and Exo1 repairs stalled replication forks poorly without EEPD1. Thus, EEPD1 performs a gatekeeper function for replication fork repair by mediating the fork cleavage that permits initiation of HR-mediated repair and restart of stressed forks. PMID:28049724

24. View of one of the plaques from Clark Fork ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. View of one of the plaques from Clark Fork Vehicle Bridge. Presently located at the Bonner County Historical Museum in Sandpoint, Idaho. A plaque was attached at each end of the bridge. Only one remains. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

77 FR 76420 - Final Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-28

... Fort Gay, confluence. Unincorporated Areas of Wayne County. At the Tug Fork +575 confluence. Mill Creek (backwater effects from From the Tug Fork +575 Town of Fort Gay. Tug Fork). confluence to approximately 1.1 miles upstream of the Tug Fork confluence. Tug Fork At the Big Sandy River +575 Town of Fort Gay...

A chirp, a roar and a whisper

NASA Astrophysics Data System (ADS)

Wijers, Ralph

2018-02-01

In 2017, gravitational waves and electromagnetic radiation were detected from the merger of two stellar remnants called neutron stars. An observational analysis reveals how this radiation was released from the merger.

Dry demagnetization cryostat for sub-millikelvin helium experiments: Refrigeration and thermometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Todoshchenko, I., E-mail: todo@boojum.hut.fi; Kaikkonen, J.-P.; Hakonen, P. J.

We demonstrate successful “dry” refrigeration of quantum fluids down to T = 0.16 mK by using copper nuclear demagnetization stage that is pre-cooled by a pulse-tube-based dilution refrigerator. This type of refrigeration delivers a flexible and simple sub-mK solution to a variety of needs including experiments with superfluid {sup 3}He. Our central design principle was to eliminate relative vibrations between the high-field magnet and the nuclear refrigeration stage, which resulted in the minimum heat leak of Q = 4.4 nW obtained in field of 35 mT. For thermometry, we employed a quartz tuning fork immersed into liquid {sup 3}He. We show that themore » fork oscillator can be considered as self-calibrating in superfluid {sup 3}He at the crossover point from hydrodynamic into ballistic quasiparticle regime.« less

Male-specific coliphages for source tracking fecal contamination in irrigation waters and prevalence of Shiga toxigenic Escherichia coli in a major produce production region of central coast of California

USDA-ARS?s Scientific Manuscript database

Determining the environmental sources for Shiga toxigenic Escherichia coli is of paramount importance. Since dairy or feedlot cattle are likely sources for this pathogen, determining the sources of fecal contamination may provide supplemental data to traditional trace-back studies from fork to farm....

Appalachian hardwood stump sprouts are potential sawlog crop trees

Treesearch

Neil I. Lamson

1976-01-01

A survey of 8- and 12-year-old hardwood stump sprouts was made in north-central West Virginia. Species surveyed were yellow-poplar, black cherry, red oak, red maple, and basswood. Of the stumps cut 12 years ago, 66 percent produced at least one dominant or codominant sprout that originated at groundline and was free from forks in the lower 25 feet of the bole. The...

Tree Survival and Growth Following Ice Storm Injury

Treesearch

Walter C. Shortle; Kevin T. Smith; Kenneth R. Dudzik

2003-01-01

Nearly 25 million acres of forest from northwestern New York and southern Quebec to the south-central Maine coast were coated with ice from a 3-day storm in early January 1998. This storm was unusual in its size and the duration of icing. Trees throughout the region were injured as branches and stems broke and forks split under the weight of the ice. These injuries...

Both DNA Polymerases δ and ε Contact Active and Stalled Replication Forks Differently

PubMed Central

Yu, Chuanhe; Gan, Haiyun

2017-01-01

ABSTRACT Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase with active and stalled replication forks. Here, we show that each DNA polymerase has a distinct pattern of association with active and stalled replication forks. Pol α is enriched at extending Okazaki fragments of active and stalled forks. In contrast, although Pol δ contacts the nascent lagging strands of active and stalled forks, it binds to only the matured (and not elongating) Okazaki fragments of stalled forks. Pol ε has greater contact with the nascent single-stranded DNA (ssDNA) of the leading strand on active forks than on stalled forks. We propose that the configuration of DNA polymerases at stalled forks facilitates the resumption of DNA synthesis after stress removal. PMID:28784720

Better Ear Health

MedlinePlus

... loss related to otitis media can be alleviated. Tinnitus Tinnitus is the medical name indicating “ringing in the ... loud roaring to clicking, humming, or buzzing. Most tinnitus comes from damage to the microscopic endings of ...

Rinne revisited: steel versus aluminum tuning forks.

PubMed

MacKechnie, Cheryl A; Greenberg, Jesse J; Gerkin, Richard C; McCall, Andrew A; Hirsch, Barry E; Durrant, John D; Raz, Yael

2013-12-01

(1) Determine whether tuning fork material (aluminum vs stainless steel) affects Rinne testing in the clinical assessment of conductive hearing loss (CHL). (2) Determine the relative acoustic and mechanical outputs of 512-Hz tuning forks made of aluminum and stainless steel. Prospective, observational. Outpatient otology clinic. Fifty subjects presenting May 2011 to May 2012 with negative or equivocal Rinne in at least 1 ear and same-day audiometry. Rinne test results using aluminum and steel forks were compared and correlated with the audiometric air-bone gap. Bench top measurements using sound-level meter, microphone, and artificial mastoid. Patients with CHL were more likely to produce a negative Rinne test with a steel fork than with an aluminum fork. Logistic regression revealed that the probability of a negative Rinne reached 50% at a 19 dB air-bone gap for stainless steel versus 27 dB with aluminum. Bench top testing revealed that steel forks demonstrate, in effect, more comparable air and bone conduction efficiencies while aluminum forks have relatively lower bone conduction efficiency. We have found that steel tuning forks can detect a lesser air-bone gap compared to aluminum tuning forks. This is substantiated by observations of clear differences in the relative acoustic versus mechanical outputs of steel and aluminum forks, reflecting underlying inevitable differences in acoustic versus mechanical impedances of these devices, and thus efficiency of coupling sound/vibratory energy to the auditory system. These findings have clinical implications for using tuning forks to determine candidacy for stapes surgery.

8 Areas of Age-Related Change

MedlinePlus

... as they get older. The decline is slow. Tinnitus (tih-NIE-tuhs) accompanies many forms of hearing ... those that sometimes come with aging. People with tinnitus may hear a ringing, roaring, or some other ...

Sunrayce 97 Finish Sets Records

Science.gov Websites

in Indianapolis,, Sunrayce 97 roared to a record finish in Colorado Springs. Winning the event , followed closely by the University of Waterloo, and the University of Minnesota. The event started in

Age Estimation of African Lions Panthera leo by Ratio of Tooth Areas

PubMed Central

Ikanda, Dennis; Ferrante, Luigi; Chardonnet, Philippe; Mesochina, Pascal; Cameriere, Roberto

2016-01-01

Improved age estimation of African lions Panthera leo is needed to address a number of pressing conservation issues. Here we present a formula for estimating lion age to within six months of known age based on measuring the extent of pulp closure from X-rays, or Ratio Of tooth AReas (ROAR). Derived from measurements taken from lions aged 3–13 years for which exact ages were known, the formula explains 92% of the total variance. The method of calculating the pulp/tooth area ratio, which has been used extensively in forensic science, is novel in the study of lion aging. As a quantifiable measure, ROAR offers improved lion age estimates for population modeling and investigations of age-related mortality, and may assist national and international wildlife authorities in judging compliance with regulatory measures involving age. PMID:27089506

Age Estimation of African Lions Panthera leo by Ratio of Tooth Areas.

PubMed

White, Paula A; Ikanda, Dennis; Ferrante, Luigi; Chardonnet, Philippe; Mesochina, Pascal; Cameriere, Roberto

2016-01-01

Improved age estimation of African lions Panthera leo is needed to address a number of pressing conservation issues. Here we present a formula for estimating lion age to within six months of known age based on measuring the extent of pulp closure from X-rays, or Ratio Of tooth AReas (ROAR). Derived from measurements taken from lions aged 3-13 years for which exact ages were known, the formula explains 92% of the total variance. The method of calculating the pulp/tooth area ratio, which has been used extensively in forensic science, is novel in the study of lion aging. As a quantifiable measure, ROAR offers improved lion age estimates for population modeling and investigations of age-related mortality, and may assist national and international wildlife authorities in judging compliance with regulatory measures involving age.

Nonlinear whistler wave model for lion roars in the Earth's magnetosheath

NASA Astrophysics Data System (ADS)

Dwivedi, N. K.; Singh, S.

2017-09-01

In the present study, we construct a nonlinear whistler wave model to explain the magnetic field spectra observed for lion roars in the Earth's magnetosheath region. We use two-fluid theory and semi-analytical approach to derive the dynamical equation of whistler wave propagating along the ambient magnetic field. We examine the magnetic field localization of parallel propagating whistler wave in the intermediate beta plasma applicable to the Earth's magnetosheath. In addition, we investigate spectral features of the magnetic field fluctuations and the spectral slope value. The magnetic field spectrum obtained by semi-analytical approach shows a spectral break point and becomes steeper at higher wave numbers. The observations of IMP 6 plasma waves and magnetometer experiment reveal the existence of short period magnetic field fluctuations in the magnetosheath. The observation shows the broadband spectrum with a spectral slope of -4.5 superimposed with a narrow band peak. The broadband fluctuations appear due to the energy cascades attributed by low-frequency magnetohydrodynamic modes, whereas, a narrow band peak is observed due to the short period lion roars bursts. The energy spectrum predicted by the present theoretical model shows a similar broadband spectrum in the wave number domain with a spectral slope of -3.2, however, it does not show any narrow band peak. Further, we present a comparison between theoretical energy spectrum and the observed spectral slope in the frequency domain. The present semi-analytical model provides exposure to the whistler wave turbulence in the Earth's magnetosheath.

Water quality, organic chemistry of sediment, and biological conditions of streams near an abandoned wood-preserving plant site at Jackson, Tennessee

USGS Publications Warehouse

Bradfield, A.D.; Flexner, N.M.; Webster, D.A.

1993-01-01

An investigation of water quality, organic sediment chemistry, and biological conditions of streams near an abandoned wood-preserving plant site at Jackson, Tennessee, was conducted during December 1990. The study was designed to assess the extent of possible contamination of water and biota in the streams from creosote-related discharge originating at this Superfund site. Central Creek, adjacent to the plant, had degraded water quality and biological conditions. Water samples from the most downstream station on Central Creek contained 30 micrograms per liter of pentachlorophenol, which exceeds the State's criterion maximum concentrations of 9 micrograms per liter for fish and aquatic life. Bottom-sediment samples from stations on Central Creek contained concentrations of acenaphthene, napthalene, and phenanthrene ranging from 1,400 to 2,500 micrograms per kilogram. Chronic or acute toxicity resulted during laboratory experiments using test organisms exposed to creosote-related contaminants. Sediment elutriate samples from Central Creek caused slightly to highly toxic effects on Ceriodaphnia dubia. Pimephales promelas, and Photobacterium phosphoreum. Fish-tissue samples from this station contained concentrations of naphthalene. dibenzofuran, fluorene, and phenanthrene ranging from 1.5 to 3.9 micrograms per kilogram Blue-green algae at this station represented about 79 percent of the organisms counted, whereas diatoms accounted for only 11 percent. Benthic invertebrate and fish samples from Central Creek had low diversity and density. Sediment samples from a station on the South Fork Forked Deer River downstream from its confluence with Central Creek contained concentrations of acenaphthene, anthracene, chrysene, fluoranthene, fluorene, pyrere, and phenanthrene ranging from 2,800 to 69,000 micrograms per kilogram. Sediment elutriate samples using water as elutriate from this station contained concentrations of extractable organic compounds ranging from an estimated 43 to 420 micrograms per liter. Sediment elutriate samples were toxic to Ceriodaphnia dubta, Pimephales promelas, Photobacterium phosphoreum, and Salenastrum capricornulum.

Using a Forest Health Index as an Outreach Tool for Improving Public Understanding of Ecosystem Dynamics and Research-Based Management

NASA Astrophysics Data System (ADS)

Osenga, E. C.; Cundiff, J.; Arnott, J. C.; Katzenberger, J.; Taylor, J. R.; Jack-Scott, E.

2015-12-01

An interactive tool called the Forest Health Index (FHI) has been developed for the Roaring Fork watershed of Colorado, with the purpose of improving public understanding of local forest management and ecosystem dynamics. The watershed contains large areas of White River National Forest, which plays a significant role in the local economy, particularly for recreation and tourism. Local interest in healthy forests is therefore strong, but public understanding of forest ecosystems is often simplified. This can pose challenges for land managers and researchers seeking a scientifically informed approach to forest restoration, management, and planning. Now in its second iteration, the FHI is a tool designed to help bridge that gap. The FHI uses a suite of indicators to create a numeric rating of forest functionality and change, based on the desired forest state in relation to four categories: Ecological Integrity, Public Health and Safety, Ecosystem Services, and Sustainable Use and Management. The rating is based on data derived from several sources including local weather stations, stream gauge data, SNOTEL sites, and National Forest Service archives. In addition to offering local outreach and education, this project offers broader insight into effective communication methods, as well as into the challenges of using quantitative analysis to rate ecosystem health. Goals of the FHI include its use in schools as a means of using local data and place-based learning to teach basic math and science concepts, improved public understanding of ecological complexity and need for ongoing forest management, and, in the future, its use as a model for outreach tools in other forested communities in the Intermountain West.

The fecundity of fork-tailed threadfin bream (Nemipterus furcosus) in Bangka, Bangka Belitung

NASA Astrophysics Data System (ADS)

Utami, E.; Safitriyani, E.; Gatra Persada, Leo

2018-04-01

Fork-tailed threadfin bream (Nemipterus furcosus) is one of important economic fishes in Bangka. The sustainability of fork-tailed threadfin bream is threatened by degradation of natural habitat. Information of reproductive is needed for further management. The objective of this study was to examine fecundity of fork-tailed threadfin bream. The mean values of temperature was 28.83 ± 0,37°C, respectively. Sex ratio during sampling showed that female fork-tailed threadfin bream greater than male population. Berried female fork-tailed threadfin bream found from March until November. The greatest number of berried female fork-tailed threadfin bream showed in July with berried female value of 25. Fork-tailed threadfin bream fecundity was 19951 and 66628, respectively. The fecundity data can be used to access the reproductive potential of fish stock and also as an assessment on stock size of their natural population.

Inter-Fork Strand Annealing causes genomic deletions during the termination of DNA replication.

PubMed

Morrow, Carl A; Nguyen, Michael O; Fower, Andrew; Wong, Io Nam; Osman, Fekret; Bryer, Claire; Whitby, Matthew C

2017-06-06

Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. Replication fork barriers are a commonly encountered problem, which can cause fork collapse and act as hotspots for replication termination. Collapsed forks can be rescued by homologous recombination, which restarts replication. However, replication restart is relatively slow and, therefore, replication termination may frequently occur by an active fork converging on a collapsed fork. We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. Based on our findings, we propose that IFSA is a potential threat to genomic stability in eukaryotes.

Termination of DNA replication forks: "Breaking up is hard to do".

PubMed

Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

2015-01-01

To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component - Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field.

Sortal Concepts and Pragmatic Inference in Children's Early Quantification of Objects

ERIC Educational Resources Information Center

Srinivasan, Mahesh; Chestnut, Eleanor; Li, Peggy; Barner, David

2013-01-01

It is typically assumed that count nouns like "fork" act as logical sortals, specifying whether objects are countable units of a kind (e.g., that a whole fork counts as "one fork") or not (e.g., that a piece of a fork does not count as "one fork"). In four experiments, we provide evidence from linguistic and conceptual development that nouns do…

11. Photocopy of photograph (original copy in Edison collection). Photographer ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Photocopy of photograph (original copy in Edison collection). Photographer and date unknown, although photo taken prior to 1930 reconstruction of Project flumes. VIEW OF ORIGINAL SOUTH FORK OF THE TULE RIVER MIDDLE FORK "BOX" WOOD FLUME BRANCH SHOWING NORTH FORK OF TULE RIVER MIDDLE FORK CROSSING. VIEW TO NORTHWEST. - Tule River Hydroelectric Project, Water Conveyance System, Middle Fork Tule River, Springville, Tulare County, CA

Grand Forks/East Grand Forks ITS strategy plan

DOT National Transportation Integrated Search

2001-01-15

The Grand Forks/East Grand Forks (GF/EGF) Area's Intelligent Transportation Systems (ITS) Strategy Plan is an effort by the GF/EGF Metropolitan Planning Organization (MPO) and its partners to develop a plan for deploying Intelligent Transportation Sy...

77 FR 39675 - Wallowa-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-05

...-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining AGENCY: Forest Service, USDA... North Fork Burnt River Mining Record of Decision will replace and supercede the 2004 North Fork Burnt...

Replication stress-induced chromosome breakage is correlated with replication fork progression and is preceded by single-stranded DNA formation.

PubMed

Feng, Wenyi; Di Rienzi, Sara C; Raghuraman, M K; Brewer, Bonita J

2011-10-01

Chromosome breakage as a result of replication stress has been hypothesized to be the direct consequence of defective replication fork progression, or "collapsed" replication forks. However, direct and genome-wide evidence that collapsed replication forks give rise to chromosome breakage is still lacking. Previously we showed that a yeast replication checkpoint mutant mec1-1, after transient exposure to replication impediment imposed by hydroxyurea (HU), failed to complete DNA replication, accumulated single-stranded DNA (ssDNA) at the replication forks, and fragmented its chromosomes. In this study, by following replication fork progression genome-wide via ssDNA detection and by direct mapping of chromosome breakage after HU exposure, we have tested the hypothesis that the chromosome breakage in mec1 cells occurs at collapsed replication forks. We demonstrate that sites of chromosome breakage indeed correlate with replication fork locations. Moreover, ssDNA can be detected prior to chromosome breakage, suggesting that ssDNA accumulation is the common precursor to double strand breaks at collapsed replication forks.

General perspective view of the North Fork Butter Creek Bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

General perspective view of the North Fork Butter Creek Bridge, view looking southwest - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Approach view of the North Fork Butter Creek Bridge, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Approach view of the North Fork Butter Creek Bridge, view looking south - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

General perspective view of the North Fork Butter Creek Bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

General perspective view of the North Fork Butter Creek Bridge, view looking north - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Elevation view of the North Fork Butter Creek Bridge, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Elevation view of the North Fork Butter Creek Bridge, view looking west - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Approach view of the North Fork Butter Creek Bridge, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Approach view of the North Fork Butter Creek Bridge, view looking north - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Detail perspective view of the North Fork Butter Creek Bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail perspective view of the North Fork Butter Creek Bridge, view looking southwest - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

General perspective view of the North Fork Butter Creek Bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

General perspective view of the North Fork Butter Creek Bridge, view looking south - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Genetics Home Reference: Ménière disease

MedlinePlus

... dizziness (vertigo), a roaring sound in the ears (tinnitus), a feeling of pressure or fullness in the ... many affected individuals develop ongoing problems with unsteadiness, tinnitus, and a feeling of fullness in the ears. ...

When and How to Take Antibiotics

MedlinePlus

... Work Contact Us ABOUT THE ISSUE What is Antibiotic Resistance? General Background Science of Resistance Glossary References POLICY ... for Adaptation Genetics and Drug Resistance Reservoirs of Antibiotic Resistance Project (ROAR) INTERNATIONAL CHAPTERS APUA Chapter Network Africa ...

Assessment of hand-transmitted vibration exposure from motorized forks used for beach-cleaning operations.

PubMed

McDowell, Thomas W; Welcome, Daniel E; Warren, Christopher; Xu, Xueyan S; Dong, Ren G

2013-01-01

Motorized vibrating manure forks were used in beach-cleaning operations following the massive Deepwater Horizon oil spill in the Gulf of Mexico during the summer of 2010. The objectives of this study were to characterize the vibration emissions of these motorized forks and to provide a first approximation of hand-transmitted vibration exposures to workers using these forks for beach cleaning. Eight operators were recruited to operate the motorized forks during this laboratory study. Four fork configurations were used in the study; two motor speeds and two fork basket options were evaluated. Accelerations were measured near each hand as the operators completed the simulated beach-cleaning task. The dominant vibration frequency for these tools was identified to be around 20 Hz. Because acceleration was found to increase with motor speed, workers should consider operating these tools with just enough speed to get the job done. These forks exhibited considerable acceleration magnitudes when unloaded. The study results suggest that the motor should not be operated with the fork in the unloaded state. Anti-vibration gloves are not effective at attenuating the vibration frequencies produced by these forks, and they may even amplify the transmitted vibration and increase hand/arm fatigue. While regular work gloves are suitable, vibration-reducing gloves may not be appropriate for use with these tools. These considerations may also be generally applicable for the use of motorized forks in other workplace environments.

Note: Enhanced energy harvesting from low-frequency magnetic fields utilizing magneto-mechano-electric composite tuning-fork.

PubMed

Yang, Aichao; Li, Ping; Wen, Yumei; Yang, Chao; Wang, Decai; Zhang, Feng; Zhang, Jiajia

2015-06-01

A magnetic-field energy harvester using a low-frequency magneto-mechano-electric (MME) composite tuning-fork is proposed. This MME composite tuning-fork consists of a copper tuning fork with piezoelectric Pb(Zr(1-x)Ti(x))O3 (PZT) plates bonded near its fixed end and with NdFeB magnets attached at its free ends. Due to the resonance coupling between fork prongs, the MME composite tuning-fork owns strong vibration and high Q value. Experimental results show that the proposed magnetic-field energy harvester using the MME composite tuning-fork exhibits approximately 4 times larger maximum output voltage and 7.2 times higher maximum power than the conventional magnetic-field energy harvester using the MME composite cantilever.

Topographic view of the North Fork Butter Creek Bridge (located ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Topographic view of the North Fork Butter Creek Bridge (located center of frame), view looking west - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks.

PubMed

Elia, Andrew E H; Wang, David C; Willis, Nicholas A; Boardman, Alexander P; Hajdu, Ildiko; Adeyemi, Richard O; Lowry, Elizabeth; Gygi, Steven P; Scully, Ralph; Elledge, Stephen J

2015-10-15

We have used quantitative proteomics to profile ubiquitination in the DNA damage response (DDR). We demonstrate that RPA, which functions as a protein scaffold in the replication stress response, is multiply ubiquitinated upon replication fork stalling. Ubiquitination of RPA occurs on chromatin, involves sites outside its DNA binding channel, does not cause proteasomal degradation, and increases under conditions of fork collapse, suggesting a role in repair at stalled forks. We demonstrate that the E3 ligase RFWD3 mediates RPA ubiquitination. RFWD3 is necessary for replication fork restart, normal repair kinetics during replication stress, and homologous recombination (HR) at stalled replication forks. Mutational analysis suggests that multisite ubiquitination of the entire RPA complex is responsible for repair at stalled forks. Multisite protein group sumoylation is known to promote HR in yeast. Our findings reveal a similar requirement for multisite protein group ubiquitination during HR at stalled forks in mammalian cells. Copyright © 2015 Elsevier Inc. All rights reserved.

Feasibility and safety of modified inverted T-shaped method using linear stapler with movable cartridge fork for esophagojejunostomy following laparoscopic total gastrectomy.

PubMed

Ohuchida, Kenoki; Nagai, Eishi; Moriyama, Taiki; Shindo, Koji; Manabe, Tatsuya; Ohtsuka, Takao; Shimizu, Shuji; Nakamura, Masafumi

2017-01-01

We previously reported the use of an inverted T-shaped method to obtain a suitable view for hand sewing to close the common entry hole when the linear stapler was fired for esophagojejunostomy after laparoscopic total gastrectomy (LTG). This conventional method involved insertion of the fixed cartridge fork to the Roux limb and the fine movable anvil fork to the esophagus to avoid perforation of the jejunum. However, insertion of the movable anvil fork to the esophagus during this procedure often requires us to strongly push down the main body of the stapler with the fixed cartridge fork to bring the direction of the anvil fork in line with the direction of the long axis of the esophagus while controlling the opening of the movable anvil fork. We therefore modified this complicated inverted T-shaped method using a linear stapler with a movable cartridge fork. This modified method involved insertion of the movable cartridge fork into the Roux limb followed by natural, easy insertion of the fixed anvil fork into the esophagus without controlling the opening of the movable cartridge fork. We performed LTG in a total of 155 consecutive patients with gastric cancer from November 2007 to December 2015 in Kyushu University Hospital. After LTG, we performed the conventional inverted T-shaped method using a linear stapler with a fixed cartridge fork in 61 patients from November 2007 to July 2011 (fixed cartridge group). From August 2011, we used a linear stapler with a movable cartridge fork and performed the modified inverted T-shaped method in 94 patients (movable cartridge group). We herein compare the short-term outcomes in 94 cases of LTG using the modified method (movable cartridge fork) with those in 61 cases using the conventional method (fixed cartridge fork). We found no significant differences in the perioperative or postoperative events between the movable and fixed cartridge groups. One case of anastomotic leakage occurred in the fixed cartridge group, but no anastomotic leakage occurred in the movable cartridge group. Although there were no remarkable differences in the short-term outcomes between the movable and fixed cartridge groups, we believe that the modified inverted T-shaped method is technically more feasible and reliable than the conventional method and will contribute to the improved safety of LTG.

Feasibility and safety of modified inverted T-shaped method using linear stapler with movable cartridge fork for esophagojejunostomy following laparoscopic total gastrectomy

PubMed Central

Ohuchida, Kenoki; Moriyama, Taiki; Shindo, Koji; Manabe, Tatsuya; Ohtsuka, Takao; Shimizu, Shuji; Nakamura, Masafumi

2017-01-01

Background We previously reported the use of an inverted T-shaped method to obtain a suitable view for hand sewing to close the common entry hole when the linear stapler was fired for esophagojejunostomy after laparoscopic total gastrectomy (LTG). This conventional method involved insertion of the fixed cartridge fork to the Roux limb and the fine movable anvil fork to the esophagus to avoid perforation of the jejunum. However, insertion of the movable anvil fork to the esophagus during this procedure often requires us to strongly push down the main body of the stapler with the fixed cartridge fork to bring the direction of the anvil fork in line with the direction of the long axis of the esophagus while controlling the opening of the movable anvil fork. We therefore modified this complicated inverted T-shaped method using a linear stapler with a movable cartridge fork. This modified method involved insertion of the movable cartridge fork into the Roux limb followed by natural, easy insertion of the fixed anvil fork into the esophagus without controlling the opening of the movable cartridge fork. Methods We performed LTG in a total of 155 consecutive patients with gastric cancer from November 2007 to December 2015 in Kyushu University Hospital. After LTG, we performed the conventional inverted T-shaped method using a linear stapler with a fixed cartridge fork in 61 patients from November 2007 to July 2011 (fixed cartridge group). From August 2011, we used a linear stapler with a movable cartridge fork and performed the modified inverted T-shaped method in 94 patients (movable cartridge group). We herein compare the short-term outcomes in 94 cases of LTG using the modified method (movable cartridge fork) with those in 61 cases using the conventional method (fixed cartridge fork). Results We found no significant differences in the perioperative or postoperative events between the movable and fixed cartridge groups. One case of anastomotic leakage occurred in the fixed cartridge group, but no anastomotic leakage occurred in the movable cartridge group. Conclusions Although there were no remarkable differences in the short-term outcomes between the movable and fixed cartridge groups, we believe that the modified inverted T-shaped method is technically more feasible and reliable than the conventional method and will contribute to the improved safety of LTG. PMID:28616606

[History of the tuning fork. I: Invention of the tuning fork, its course in music and natural sciences. Pictures from the history of otorhinolaryngology, presented by instruments from the collection of the Ingolstadt German Medical History Museum].

PubMed

Feldmann, H

1997-02-01

G. Cardano, physician, mathematician, and astrologer in Pavia, Italy, in 1550 described how sound may be perceived through the skull. A few years later H. Capivacci, also a physician in Padua, realized that this phenomenon might be used as a diagnostic tool for differentiating between hearing disorders located either in the middle ear or in the acoustic nerve. The German physician G. C. Schelhammer in 1684 was the first to use a common cutlery fork in further developing the experiments initiated by Cardano and Capivacci. For a long time to come, however, there was no demand for this in practical otology. The tuning fork was invented in 1711 by John Shore, trumpeter and lutenist to H. Purcell and G.F. Händel in London. A picture of Händel's own tuning fork, probably the oldest tuning fork in existence, is presented here for the first time. There are a number of anecdotes connected with the inventor of the tuning fork, using plays on words involving the name Shore, and mixing up pitch-pipe and pitchfork. Some of these are related here. The tuning fork as a musical instrument soon became a success throughout Europe. The German physicist E. F. F. Chladni in Wittenberg around 1800 was the first to systematically investigate the mode of vibration of the tuning fork with its nodal points. Besides this, he and others tried to construct a complete musical instrument based on sets of tuning forks, which, however, were not widely accepted. J. H. Scheibler in Germany in 1834 presented a set of 54 tuning forks covering the range from 220 Hz to 440 Hz, at intervals of 4 Hz. J. Lissajous in Paris constructed a very elaborate tuning fork with a resonance box, which was intended to represent the international standard of the musical note A with 435 vibrations per second, but this remained controversial. K. R. Koenig, a German physicist living in Paris, invented a tuning fork which was kept in continuous vibration by a clockwork. H. Helmholtz, physiologist in Heidelberg, in 1863 used sets of electromagnetically powered tuning forks for his famous experiments on the sensations of tone. Until the invention of the electronic valve, tuning forks remained indispensible instruments for producing defined sinusoidal vibrations. The history of this development is presented in detail. The diagnostic use of the tuning fork in otology will be described in a separate article.

Statistical Study of the Properties of Magnetosheath Lion Roars using MMS observations

NASA Astrophysics Data System (ADS)

Giagkiozis, S.; Wilson, L. B., III

2017-12-01

Intense whistler-mode waves of very short duration are frequently encountered in the magnetosheath. These emissions have been linked to mirror mode waves and the Earth's bow shock. They can efficiently transfer energy between different plasma populations. These electromagnetic waves are commonly referred to as Lion roars (LR), due to the sound generated when the signals are sonified. They are generally observed during dips of the magnetic field that are anti-correlated with increases of density. Using MMS data, we have identified more than 1750 individual LR burst intervals. Each emission was band-pass filtered and further split into >35,000 subintervals, for which the direction of propagation and the polarization were calculated. The analysis of subinterval properties provides a more accurate representation of their true nature than the more commonly used time- and frequency-averaged dynamic spectra analysis. The results of the statistical analysis of the wave properties will be presented.

New insights into sub-ion scale turbulence in Earth's magnetosheath using MMS data

NASA Astrophysics Data System (ADS)

Breuillard, Hugo; Andriopoulou, Maria; Graham, Daniel; Le Contel, Olivier; Huang, Shiyong; Hadid, Lina; Sahraoui, Fouad; Alexandrova, Olga; Berthomier, Matthieu; Retino, Alessandro; Nakamura, Rumi; Baumjohann, Wolfgang

2017-04-01

On January 22nd 2016, MMS was located in Earth's magnetosheath and detected intense lion roars showing a secondary bandwidth. Detailed polarization analysis, using burst data from SCM and EDP instruments, and numerical simulation, using WHAMP, are performed in this study. They show that these mainly perpendicular fluctuations are highly nonlinear whistler wave packets, and that a high sampling rate is needed to pick up the peaks of the signal. As a result, their amplitude might have been underestimated in previous missions such as Cluster, which can have a significant impact on electron dynamics. Using FPI burst data, we show that electron velocity distribution functions exhibit a gyrophase-bunched signature in the presence of these lion roars. The analysis of magnetic and density fluctuations, inferred from spacecraft potential, also show the highly-compressible nature of turbulence up to electron scales.

Magnetosheath lion roars

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, E.J.; Tsurutani, B.T.

1976-05-01

Lion roars, which are intense packets of electromagnetic waves characteristically found in the magneosheath, have been studied. On the basis of these observations, several possible wave generation mechanisms are examined. Landau resonance is considered to be an unlikely source because this mechanism requires a substantial component of the wave electric field paralle to B, and the observation that the waves propagate along the ambient field is contrary to this requirement. It is not obvious that electron cyclotron resonance is responsible, because the field magnitude decreases should cause T/sub parallel//T/sub perpendicular/ to increase, and this rise could lead to wave dampingmore » rather than wave growth. A model which is consistent with all the observations of this study is a proton cyclotron overstability involving 10-keV protons streaming through the magnetosheath. It appears possible that the streaming protons could produce both the waves and the field decreases and that all three would be coincident. (AIP)« less

Investigating the Role of Helicobacter pylori PriA Protein.

PubMed

Singh, Aparna; Blaskovic, Dusan; Joo, Jungsoo; Yang, Zhen; Jackson, Sharon H; Coleman, William G; Yan, Ming

2016-08-01

In bacteria, PriA protein, a conserved DEXH-type DNA helicase, plays a central role in replication restart at stalled replication forks. Its unique DNA binding property allows it to recognize and stabilize stalled forks and the structures derived from them. PriA plays a very critical role in replication fork stabilization and DNA repair in E. coli and N. gonorrhoeae. In our in vivo expression technology screen, priA gene was induced in vivo when Helicobacter pylori infects mouse stomach. We decided to elucidate the role of H. pylori PriA protein in survival in mouse stomach, survival in gastric epithelial cells and macrophage cells, DNA repair, acid stress, and oxidative stress. The priA null mutant strain was unable to colonize mice stomach mucosa after long-term infections. Mouse colonization was observed after 1 week of infection, but the levels were much lower than the wild-type HpSS1 strain. PriA protein was found to be important for intracellular survival of epithelial cell-/macrophage cell-ingested H. pylori. Also, a priA null mutant was more sensitive to DNA-damaging agents and was much more sensitive to acid and oxidative stress as compared to the wild-type strain. These data suggest that the PriA protein is needed for survival and persistence of H. pylori in mice stomach mucosa. © 2016 John Wiley & Sons Ltd.

Naturally fractured tight gas reservoir detection optimization. Quarterly report, April--June 1994

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1994-07-01

Geologic assessment of the basin during the third quarter possessed several major objectives. The first task was to test the validity of the gas-centered basin model for the Piceance Basin. The second objective was to define the location and variability of gas-saturated zones within the Williams Fork and Iles Formation reservoir horizons. A third objective was to prepare an updated structure map of the Piceance Basin on the top of the Iles Formation (Rollins Sandstone) to take advantage of new data provided by ten years of drilling activity throughout the basin. The first two objectives formed the core of themore » ARI poster session presented at the AAPG annual meeting in Denver. The delineation of the gas and water-saturated zone geometries for the Williams Fork and Iles Formations in the basin was presented in the form of a poster session at the AAPG Annual meeting held in Denver in mid-June. The poster session outlined the nature of the gas-centered basin geometry and demonstrated the gas and water-saturated conditions for the Williams Fork, Cozzette and Corcoran reservoir horizons throughout the basin. Initial and cumulative production data indicate that these reservoir horizons are gas-saturated in most of the south-central and eastern basin. The attached report summarizes the data and conclusions of the poster session.« less

21 CFR 882.1525 - Tuning fork.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning fork...

21 CFR 882.1525 - Tuning fork.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning fork...

36 CFR 292.62 - Valid existing rights.

Code of Federal Regulations, 2010 CFR

2010-07-01

... RECREATION AREAS Smith River National Recreation Area § 292.62 Valid existing rights. (a) Definition. For the... “wild” segments of the Wild and Scenic Smith River, Middle Fork Smith River, North Fork Smith River, Siskiyou Fork Smith River, South Fork Smith River, and their designated tributaries, except Peridotite...

Reconnaissance Geologic Map of the Hayfork 15' Quadrangle, Trinity County, California

USGS Publications Warehouse

Irwin, William P.

2010-01-01

The Hayfork 15' quadrangle is located just west of the Weaverville 15' quadrangle in the southern part of the Klamath Mountains geologic province of northern California. It spans parts of six generally north-northwest-trending tectonostratigraphic terranes that are, from east to west, the Eastern Klamath, Central Metamorphic, North Fork, Eastern Hayfork, Western Hayfork, and Rattlesnake Creek terranes. Remnants of a once-widespread postaccretionary overlap assemblage, the Cretaceous Great Valley sequence, crop out at three localities in the southern part of the Hayfork quadrangle. The Tertiary fluvial and lacustrine Weaverville Formation occupies a large, shallow, east-northeast-trending graben in the south half of the quadrangle. The small area of Eastern Klamath terrane is part of the Oregon Mountain outlier, which is more widely exposed to the east in the Weaverville 15' quadrangle. It was originally mapped as a thrust plate of Bragdon(?) Formation, but it is now thought by some to be part of an outlier of Yreka terrane that has been dislocated 60 km southward by the La Grange Fault. The Central Metamorphic terrane, which forms the footwall of the La Grange Fault, was formed by the eastward subduction of oceanic crustal basalt (the Salmon Hornblende Schist) and its overlying siliceous sediments with interbedded limestone (the Abrams Mica Schist) beneath the Eastern Klamath terrane. Rb-Sr analysis of the Abrams Mica Schist indicates a Middle Devonian metamorphic age of approximately 380 Ma, which probably represents the age of subduction. The North Fork terrane, which is faulted against the western boundary of the Central Metamorphic terrane, consists of the Permian(?) North Fork ophiolite and overlying broken formation and melange of Permian to Early Jurassic (Pliensbachian) marine metasedimentary and metavolcanic rocks. The ophiolite, which crops out along the western border of the terrane, is thrust westward over the Eastern Hayfork terrane. The Eastern Hayfork terrane is a broken formation and melange of volcanic and sedimentary rocks, including chert and blocks of amphibolite, limestone, and serpentinized ultramafic rocks. The chert contains radiolarians of Permian and Triassic ages, but none of clearly Jurassic age. In contrast, the cherts of the North Fork terrane contain Early and Middle Jurassic radiolarians in addition to those of Permian and Triassic ages; also, some limestones of the Eastern Hayfork terrane contain fossil faunas of Tethyan affinity, but those of the North Fork terrane do not. The Western Hayfork terrane is an andesitic volcanic arc that was accreted to the Eastern Hayfork terrane. It consists mainly of metavolcaniclastic andesitic agglomerate and tuff, as well as argillite and chert, and it includes the dioritic Ironside Mountain batholith, which intruded during Middle Jurassic time. Two large patches of Western Hayfork terrane mapped in the central part of the Eastern Hayfork terrane may be exposed through windows in the Eastern Hayfork terrane, although the structural relation is not clear. The Rattlesnake Creek terrane is a melange that occupies only a small area in the southwest corner of the Hayfork quadrangle; however, it is a major unit in the Hyampom 15' quadrangle to the west. It consists mainly of broken and sheared ophiolitic rocks of probable Permian or early Mesozoic age. The Cretaceous Great Valley sequence overlap assemblage here postdates the Early Cretaceous (approximately 136 Ma) emplacement of the Shasta Bally batholith, which is widely exposed to the east in the Weaverville 15' quadrangle. The Great Valley sequence once covered much of the southern Klamath Mountains; however, in the Hayfork quadrangle, only three small patches remain near its southern boundary. Weakly consolidated nonmarine sedimentary rocks of the Weaverville Formation of mid-Tertiary age, which contain abundant fossil plants, occupy a large, shallow, east-northeast-trending graben

Potential effects of climate change on streamflow, eastern and western slopes of the Sierra Nevada, California and Nevada

USGS Publications Warehouse

Jeton, A.E.; Dettinger, M.D.; Smith, J. LaRue

1996-01-01

Precipitation-runoff models of the East Fork Carson and North Fork American Rivers were developed and calibrated for use in evaluating the sensitivity of streamflow in the north-central Sierra Nevada to climate change. The East Fork Carson River drains part of the rain-shadowed, eastern slope of the Sierra Nevada and is generally higher than the North Fork American River, which drains the wetter, western slope. First, a geographic information system was developed to describe the spatial variability of basin characteristics and to help estimate model parameters. The result was a partitioning of each basin into noncontiguous, but hydrologically uniform, land units. Hydrologic descriptions of these units were developed and the Precipitation- Runoff Modeling System (PRMS) was used to simulate water and energy balances for each unit in response to daily weather conditions. The models were calibrated and verified using historical streamflows over 22-year (Carson River) and 42-year (American River) periods. Simulated annual streamflow errors average plus 10 percent of the observed flow for the East Fork Carson River basin and plus 15 percent for the North Fork American River basin. Interannual variability is well simulated overall, but, at daily scales, wet periods are simulated more accurately than drier periods. The simulated water budgets for the two basins are significantly different in seasonality of streamflow, sublimation, evapotranspiration, and snowmelt. The simulations indicate that differences in snowpack and snowmelt timing can play pervasive roles in determining the sensitivity of water resources to climate change, in terms of both resource availability and amount. The calibrated models were driven by more than 25 hypothetical climate-change scenarios, each 100 years long. The scenarios were synthesized and spatially disaggregated by methods designed to preserve realistic daily, monthly, annual, and spatial statistics. Simulated streamflow timing was not very sensitive to changes in mean precipitation, but was sensitive to changes in mean temperatures. Changes in annual streamflow amounts were amplified reflections of imposed mean precipitation changes, with especially large responses to wetter climates. In contrast, streamflow amount was surprisingly insensitive to mean temperature changes as a result of temporal links between peak snowmelt and the beginning of warm-season evapotranspiration. Comparisons of simulations driven by temporally detailed climate-model changes in which mean temperature changes vary from month to month and simulations in which uniform climate changes were imposed throughout the year indicate that the snowpack accumulates the influences of short-term conditions so that season average climate changes were more important than shorter term changes.

Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism

PubMed Central

Reynolds, John J; Bicknell, Louise S; Carroll, Paula; Higgs, Martin R; Shaheen, Ranad; Murray, Jennie E; Papadopoulos, Dimitrios K; Leitch, Andrea; Murina, Olga; Tarnauskaitė, Žygimantė; Wessel, Sarah R; Zlatanou, Anastasia; Vernet, Audrey; von Kriegsheim, Alex; Mottram, Rachel MA; Logan, Clare V; Bye, Hannah; Li, Yun; Brean, Alexander; Maddirevula, Sateesh; Challis, Rachel C; Skouloudaki, Kassiani; Almoisheer, Agaadir; Alsaif, Hessa S; Amar, Ariella; Prescott, Natalie J; Bober, Michael B; Duker, Angela; Faqeih, Eissa; Seidahmed, Mohammed Zain; Al Tala, Saeed; Alswaid, Abdulrahman; Ahmed, Saleem; Al-Aama, Jumana Yousuf; Altmüller, Janine; Al Balwi, Mohammed; Brady, Angela F; Chessa, Luciana; Cox, Helen; Fischetto, Rita; Heller, Raoul; Henderson, Bertram D; Hobson, Emma; Nürnberg, Peter; Percin, E Ferda; Peron, Angela; Spaccini, Luigina; Quigley, Alan J; Thakur, Seema; Wise, Carol A; Yoon, Grace; Alnemer, Maha; Tomancak, Pavel; Yigit, Gökhan; Taylor, A Malcolm R; Reijns, Martin AM; Simpson, Michael A; Cortez, David; Alkuraya, Fowzan S; Mathew, Christopher G; Jackson, Andrew P; Stewart, Grant S

2017-01-01

To ensure efficient genome duplication, cells have evolved numerous factors that promote unperturbed DNA replication, and protect, repair and restart damaged forks. Here we identify DONSON as a novel fork protection factor, and report biallelic DONSON mutations in 29 individuals with microcephalic dwarfism. We demonstrate that DONSON is a replisome component that stabilises forks during genome replication. Loss of DONSON leads to severe replication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks. Furthermore, ATR-dependent signalling in response to replication stress is impaired in DONSON-deficient cells, resulting in decreased checkpoint activity, and potentiating chromosomal instability. Hypomorphic mutations substantially reduce DONSON protein levels and impair fork stability in patient cells, consistent with defective DNA replication underlying the disease phenotype. In summary, we identify mutations in DONSON as a common cause of microcephalic dwarfism, and establish DONSON as a critical replication fork protein required for mammalian DNA replication and genome stability. PMID:28191891

High-affinity DNA-binding Domains of Replication Protein A (RPA) Direct SMARCAL1-dependent Replication Fork Remodeling*

PubMed Central

Bhat, Kamakoti P.; Bétous, Rémy; Cortez, David

2015-01-01

SMARCAL1 catalyzes replication fork remodeling to maintain genome stability. It is recruited to replication forks via an interaction with replication protein A (RPA), the major ssDNA-binding protein in eukaryotic cells. In addition to directing its localization, RPA also activates SMARCAL1 on some fork substrates but inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reactions. We investigated the mechanism by which RPA regulates SMARCAL1. Our results indicate that although an interaction between SMARCAL1 and RPA is essential for SMARCAL1 activation, the location of the interacting surface on RPA is not. Counterintuitively, high-affinity DNA binding of RPA DNA-binding domain (DBD) A and DBD-B near the fork junction makes it easier for SMARCAL1 to remodel the fork, which requires removing RPA. We also found that RPA DBD-C and DBD-D are not required for SMARCAL1 regulation. Thus, the orientation of the high-affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity. PMID:25552480

High-affinity DNA-binding domains of replication protein A (RPA) direct SMARCAL1-dependent replication fork remodeling.

PubMed

Bhat, Kamakoti P; Bétous, Rémy; Cortez, David

2015-02-13

SMARCAL1 catalyzes replication fork remodeling to maintain genome stability. It is recruited to replication forks via an interaction with replication protein A (RPA), the major ssDNA-binding protein in eukaryotic cells. In addition to directing its localization, RPA also activates SMARCAL1 on some fork substrates but inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reactions. We investigated the mechanism by which RPA regulates SMARCAL1. Our results indicate that although an interaction between SMARCAL1 and RPA is essential for SMARCAL1 activation, the location of the interacting surface on RPA is not. Counterintuitively, high-affinity DNA binding of RPA DNA-binding domain (DBD) A and DBD-B near the fork junction makes it easier for SMARCAL1 to remodel the fork, which requires removing RPA. We also found that RPA DBD-C and DBD-D are not required for SMARCAL1 regulation. Thus, the orientation of the high-affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Areas contributing ground water to the Peconic Estuary, and ground-water budgets for the north and south forks and Shelter Island, eastern Suffolk County, New York

USGS Publications Warehouse

Schubert, C.E.

1998-01-01

The Peconic Estuary, at the eastern end of Long Island, has been plagued by a recurrent algal bloom, locally referred to as ?Brown Tide,? that has caused the severe decline of local marine resources. Although the factors that trigger Brown Tide blooms remain uncertain, groundwater discharge has previously been shown to affect surface-water quality in the western part of the estuary. A U.S. Geological Survey groundwater- flow model of the main body of Long Island indicates that a total of about 7.5 x 106 ft3/d (cubic feet per day) of freshwater discharges to the western part of the estuary, but the model does not include the ground-water flow systems on the North and South Forks and Shelter Island, which contribute significant amounts of freshwater to the central and eastern parts of the estuary. The need for information on freshwater discharge to the entire estuary prompted the U.S. Geological Survey to evaluate ground-water discharge from the North and South Forks and Shelter Island. Source areas that contribute ground water to the Peconic Estuary were delineated, and groundwater budgets for these areas were developed, to evaluate the distribution and magnitude of ground-water discharge to the central and eastern parts of the estuary. Contributing-area boundaries that were delineated coincide with the hydraulic boundaries of the fresh ground-water-flow systems of the North and South Forks and Shelter Island; these boundaries are of two types? external (saltwater bodies) and internal (groundwater divides). Hydrologic components that were evaluated include recharge from precipitation, public-supply withdrawal and return flow, and agricultural withdrawal. Values for each of these components were calculated or estimated for the individual freshwater flow subsystems that form each ground-water-budget area, then summed to obtain the total discharge of fresh ground water to tidewater. Ground-water discharge to the Peconic Estuary is about 3.8 x 106 ft3/d from the North Fork, 11 x 106 ft3/d from the South Fork, and 1.7 x 106 ft3/d from Shelter Island. The total contribution to the estuary from these areas is about 16 x 106 ft3/d?roughly twice the total contribution from the main body of Long Island. In contrast to the freshwater contribution from the main body of Long Island, which is concentrated near the head of the estuary, the contributions from the North and South Forks and Shelter Island are distributed along the east-west length of the estuary. Changes in water-table altitude and the resulting changes in total discharge to the Peconic Estuary were estimated from the relative changes in annual mean water level at observation wells. The 1985-95 interval included 7 years (1985-88, 1991- 92, 1995) of generally below-average water-table altitudes that presumably caused similar decreases in ground-water discharge to the estuary; intense Brown Tide blooms coincided with six of these years (1985-88, 1991, 1995), and localized blooms coincided with the remaining year (1992). Watertable altitudes in the remaining 4 years of the 1985-95 interval (1989-90, 1993-94) were nearly average or above average, and presumably produced comparably near-average or increased amounts of ground-water discharge to the estuary; none of these years saw any widespread Brown Tide blooms. Fluctuations in the amounts of ground-water discharge to the estuary appear to affect the occurrence of Brown Tide blooms, although the factors that trigger the blooms have not been determined.

Comparative geomorphic analysis of surficial deposits at three central Appalachian watersheds: Implications for controls on sediment-transport efficiency

NASA Astrophysics Data System (ADS)

Taylor, Stephen B.; Steven Kite, J.

2006-08-01

Factors that control the routing and storage of sediments in the Appalachian region are poorly understood. This study involves a comparative geomorphic analysis of three watersheds underlain by sandstones and shales of the Acadian clastic wedge. These areas include the Fernow Experimental Forest, Tucker County, West Virginia; the North Fork basin, Pocahontas County, West Virginia; and the Little River basin, Augusta County, Virginia. GIS-based analyses of surficial map units allow first-order approximation of sediment-storage volumes in valley bottoms. Estimates of volumes are examined in tandem with morphometric analyses and the distribution of bedrock channels to make inferences regarding controls on sediment-transport efficiency in the central Appalachians. The Fernow and North Fork areas are characterized by V-shaped valleys with mixed reaches of alluvial-bedrock channels distributed throughout the drainage network. In contrast, the Little River valley is notably wider and gravelly alluvial fill is abundant. Comparator watershed parameters for the Fernow, North Fork and Little River areas include, respectively: (1) basin area = 15.2 km 2, 49.3 km 2, 41.5 km 2; (2) basin relief = 0.586 km, 0.533 km, 0.828 km; (3) drainage density = 4.2 km - 1 , 3.3 km - 1 , 4.7 km - 1 ; (4) ruggedness = 2.5, 1.7, 3.9; (5) Shreve magnitude = 139, 287, 380; (6) total valley-bottom area (km 2) = 0.76 km 2, 1.86 km 2, 3.09 km 2; (7) average hillslope gradients = 17.2°, 18.4°, 22.1°; (8) total debris-fan surface area = 0.113 km 2, 0.165 km 2, 0.486 km 2; and (9) debris-fan frequency = 2.0 km - 2 , 1.0 km - 2 , 2.8 km - 2 . The storage volumes in valley bottoms were estimated using map polygon areas and surface heights above channel grade. The Little River contains significantly higher sediment volumes in floodplain, terrace and fan storage compartments; total volumes of the valley bottoms are approximately twice that of the Fernow and North Fork areas combined. Unit storage volumes for the Fernow, North Fork and Little River are 5.2 × 10 4 m 3 km - 2 , 5.5 × 10 4 m 3 km - 2 and 1.6 × 10 5 m 3 km - 2 , respectively. A conceptual model postulates that valley-width morphometry and style of delivery from hillslopes are the primary factors controlling the efficiency of sediment transport. Steep, debris-flow-prone hillslopes at the Little River deliver high volumes of gravelly sediment at magnitudes greater than transport capacity of the channel. Patterns of stream power are complex, as low-order tributaries are under capacity and high-order tributaries over capacity with respect to sediment load. Aggraded alluvial fill insulates valley-floor bedrock from vertical erosion and valley widening dominates. Expansion of the valley width creates a positive response via increased storage capacity and lower unit stream power. Conversely, the Fernow and North Fork are characterized by diffusive mass movement on hillslopes with incremental bedload transport to higher-order tributaries. Rates of hillslope delivery are balanced by the rate of channel export. Mixed alluvial-bedrock reaches provide the optimal channel configuration for active incision of the valley floor. Low expansion of valley width promotes high unit stream power and processes of vertical erosion. The model implies that the Fernow and North Fork have been more effective at sediment transport during the Late Quaternary. Given similar climatic and tectonic settings, variation in bedrock lithofacies is likely the primary factor modulating the efficiency of sediment transport.

Runoff generation from neighboring headwater basins with differing glacier coverage using the distributed hydrological model WaSiM, Eklutna, Alaska

NASA Astrophysics Data System (ADS)

Ostman, J. S.; Loso, M.; Liljedahl, A. K.; Gaedeke, A.; Geck, J. E.

2017-12-01

Many Alaska glaciers are thinning and retreating, and glacier wastage is projected to affect runoff processes from glacierized basins. Accordingly, effective resource management in glacierized watersheds requires quantification of a glacier's role on streamflow generation. The Eklutna catchment (311 km2) supplies water and electricity for Anchorage, Alaska (pop. 300,000) via Eklutna Lake. The Eklutna headwaters include the West Fork (64 km2, 46% glacier), and the East Fork (101 km2, 12% glacier). Total average annual discharge (2009-2015) is similar from the West (42,100 m3) and East (42,200 m3) forks, while specific annual runoff from the West Fork (2940 mm) exceeds that of the East Fork (1500 mm). To better understand what controls runoff, we are simulating the Eklutna annual water budget using a distributed watershed-level hydrological model. We force the Water Flow and Balance Simulation Model (WaSiM) using continuous air temperature, precipitation, wind speed, shortwave incoming radiation, and relative humidity primarily measured in the West Fork basin. We use Eklutna Glacier snow accumulation and ablation to calibrate the snowmelt and glacier sub-modules. Melt season discharge from the West and East forks is used for runoff comparison. Preliminary results show 2013-2015 simulated glacier point balances (accumulation and melt) are within 15% of glacier stake observations. Runoff was effectively modeled in the West Fork (NSE=0.80), while being over-predicted in the East Fork , which we attribute to a lack of forcing data in the less-glacierized basin. The simulations suggest that 78% of West Fork total runoff is from glacier melt, compared with <40% in the East Fork where glacier runoff contribution is higher during low-snow years.

Movement of bull trout in the upper Jarbidge River watershed, Idaho and Nevada, 2008-09--A supplement to Open-File Report 2010-1033

USGS Publications Warehouse

Munz, Carrie S.; Allen, M. Brady; Connolly, Patrick J.

2011-01-01

We monitored bull trout (Salvelinus confluentus) in 2008 and 2009 as a continuation of our work in 2006 and 2007, which involved the tagging of 1,536 bull trout with passive integrated transponder (PIT) tags in the East Fork Jarbidge River and West Fork Jarbidge River and their tributaries in northeastern Nevada and southern Idaho. We installed PIT tag interrogation systems (PTISs) at established locations soon after ice-out, and maintained the PTISs in order to collect information on bull trout movements through December of each year. We observed a marked increase of movement in 2008 and 2009. Bull trout tagged in the uppermost portions of the East Fork Jarbidge River at altitudes greater than 2,100 meters moved to the confluence of the East Fork Jarbidge River and West Fork Jarbidge River in summer and autumn. Ten bull trout tagged upstream of the confluence of Pine Creek and the West Fork Jarbidge River moved downstream and then upstream in the East Fork Jarbidge River, and then past the PTIS at Murphy Hot Springs (river kilometer [rkm] 4.1). Two of these fish ascended Dave Creek, a tributary of the East Fork Jarbidge River, past the PTIS at rkm 0.4. One bull trout that was tagged at rkm 11 in Dave Creek on June 28, 2007 moved downstream to the confluence of the East Fork Jarbidge River and West Fork Jarbidge River (rkm 0) on July 28, 2007, and it was then detected in the West Fork Jarbidge River moving past our PTIS at rkm 15 on May 4, 2008. Combined, the extent and types of bull trout movements observed indicated that the primarily age-1 and age-2 bull trout that we tagged in 2006 and 2007 showed increased movement with age and evidence of a substantial amount of fluvial life history. The movements suggest strong connectivity between spawning areas and downstream mainstem areas, as well as between the East Fork Jarbidge River and West Fork Jarbidge River.

Salmon Supplementation Studies in Idaho Rivers, 1999-2000 Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kohler, Andy; Taki, Doug; Teton, Angelo

2001-11-01

As part of the Idaho Supplementation Studies, fisheries crews from the Shoshone-Bannock Tribes have been snorkeling tributaries of the Salmon River to estimate chinook salmon (Oncorhynchus tshawytscha) parr abundance; conducting surveys of spawning adult chinook salmon to determine the number of redds constructed and collect carcass information; operating a rotary screw trap on the East Fork Salmon River and West Fork Yankee Fork Salmon River to enumerate and PIT-tag emigrating juvenile chinook salmon; and collecting and PIT-tagging juvenile chinook salmon on tributaries of the Salmon River. The Tribes work in the following six tributaries of the Salmon River: Bear Valleymore » Creek, East Fork Salmon River, Herd Creek, South Fork Salmon River, Valley Creek, and West Fork Yankee Fork Salmon River. Snorkeling was used to obtain parr population estimates for ISS streams from 1992 to 1997. However, using the relatively vigorous methods described in the ISS experimental design to estimate summer chinook parr populations, results on a project-wide basis showed extraordinarily large confidence intervals and coefficients of variation. ISS cooperators modified their sampling design over a few years to reduce the variation around parr population estimates without success. Consequently, in 1998 snorkeling to obtain parr population estimates was discontinued and only General Parr Monitoring (GPM) sites are snorkeled. The number of redds observed in SBT-ISS streams has continued to decline as determined by five year cycles. Relatively weak strongholds continue to occur in the South Fork Salmon River and Bear Valley Creek. A rotary screw trap was operated on the West Fork Yankee Fork during the spring and fall of 1999 and the spring of 2000 to monitor juvenile chinook migration. A screw trap was also operated on the East Fork of the Salmon River during the spring and fall from 1993 to 1997 and 1999 (fall only) to 2000. Significant supplementation treatments have occurred in the South Fork Salmon River (IDFG). The East Fork Salmon River received supplementation treatments yearly through 1995. There have been no treatments since 1995, and no significant future treatments from local broodstock are conceivable due to extremely poor escapement. The West Fork Yankee Fork received a single presmolt treatment in 1994. Similarly, no significant future treatments are planned for the WFYF due to extremely poor escapement. However, small scale experimental captive rearing and broodstock techniques are currently being tested with populations from the EFSR and WFYF. Captive rearing/broodstock techniques could potentially provide feedback for evaluation of supplementation. The other three SBT-ISS streams are control streams and do not receive hatchery treatments.« less

27 CFR 9.113 - North Fork of Long Island.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries of...

27 CFR 9.113 - North Fork of Long Island.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries of...

27 CFR 9.113 - North Fork of Long Island.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries of...

Occurrence and transport of selected constituents in streams near the Stibnite mining area, Central Idaho, 2012–14

USGS Publications Warehouse

Etheridge, Alexandra B.

2015-12-07

Ninety-eight percent of the estimated total mercury load transported downstream of the study area is attributable to Sugar Creek. A maximum concentration of 26 micrograms per liter was measured in Sugar Creek during May 2013 when snowmelt runoff occurred during a single peak in the hydrograph. Monitoring and modeling results indicate sediment and sediment-associated constituent concentrations and loads increase along Meadow Creek, likely because of the inflow of the East Fork of Meadow Creek, and decrease between sites 3 and 4 because the Glory Hole is trapping sediments. Sugar Creek (site 5) accounted for most of the sediment and sediment-associated constituent loading leaving the study area because loads from the East Fork of Meadow Creek remained trapped in the Glory Hole. Additionally, total mercury was detected at all five streamflow-gaging stations, and sampled mercury concentrations exceeded Idaho ambient water-quality criteria at all five streamflow-gaging stations.

Lambkins Roar as the Top High School in the 27th Colorado Science Bowl |

Science.gov Websites

round of the competition. As they readied to answer rapid-fire physics, math, biology, astronomy, energy group, which is an offshoot of his school's STEAM (science, technology, engineering, art, and math

Mouse embryonic stem cells have increased capacity for replication fork restart driven by the specific Filia-Floped protein complex.

PubMed

Zhao, Bo; Zhang, Weidao; Cun, Yixian; Li, Jingzheng; Liu, Yan; Gao, Jing; Zhu, Hongwen; Zhou, Hu; Zhang, Rugang; Zheng, Ping

2018-01-01

Pluripotent stem cells (PSCs) harbor constitutive DNA replication stress during their rapid proliferation and the consequent genome instability hampers their applications in regenerative medicine. It is therefore important to understand the regulatory mechanisms of replication stress response in PSCs. Here, we report that mouse embryonic stem cells (ESCs) are superior to differentiated cells in resolving replication stress. Specifically, ESCs utilize a unique Filia-Floped protein complex-dependent mechanism to efficiently promote the restart of stalled replication forks, therefore maintaining genomic stability. The ESC-specific Filia-Floped complex resides on replication forks under normal conditions. Replication stress stimulates their recruitment to stalling forks and the serine 151 residue of Filia is phosphorylated in an ATR-dependent manner. This modification enables the Filia-Floped complex to act as a functional scaffold, which then promotes the stalling fork restart through a dual mechanism: both enhancing recruitment of the replication fork restart protein, Blm, and stimulating ATR kinase activation. In the Blm pathway, the scaffolds recruit the E3 ubiquitin ligase, Trim25, to the stalled replication forks, and in turn Trim25 tethers and concentrates Blm at stalled replication forks through ubiquitination. In differentiated cells, the recruitment of the Trim25-Blm complex to replication forks and the activation of ATR signaling are much less robust due to lack of the ESC-specific Filia-Floped scaffold. Thus, our study reveals that ESCs utilize an additional and unique regulatory layer to efficiently promote the stalled fork restart and maintain genomic stability.

Effects of Transposable Elements on the Expression of the Forked Gene of Drosophila Melanogaster

PubMed Central

Hoover, K. K.; Chien, A. J.; Corces, V. G.

1993-01-01

The products of the forked gene are involved in the formation and/or maintenance of a temporary fibrillar structure within the developing bristle rudiment of Drosophila melanogaster. Mutations in the forked locus alter this structure and result in aberrant development of macrochaetae, microchaetae and trichomes. The locus has been characterized at the molecular level by walking, mutant characterization and transcript analysis. Expression of the six forked transcripts is temporally restricted to midlate pupal development. At this time, RNAs of 6.4, 5.6, 5.4, 2.5, 1.9 and 1.1 kilobases (kb) are detected by Northern analysis. The coding region of these RNAs has been found to be within a 21-kb stretch of genomic DNA. The amino terminus of the proteins encoded by the 5.4- and 5.6-kb forked transcripts contain tandem copies of ankyrin-like repeats that may play an important role in the function of forked-encoded products. The profile of forked RNA expression is altered in seven spontaneous mutations characterized during this study. Three forked mutations induced by the insertion of the gypsy retrotransposon contain a copy of this element inserted into an intron of the gene. In these mutants, the 5.6-, 5.4- and 2.5-kb forked mRNAs are truncated via recognition of the polyadenylation site in the 5' long terminal repeat of the gypsy retrotransposon. These results help explain the role of the forked gene in fly development and further our understanding of the role of transposable elements in mutagenesis. PMID:8244011

Development of Tuning Fork Based Probes for Atomic Force Microscopy

NASA Astrophysics Data System (ADS)

Jalilian, Romaneh; Yazdanpanah, Mehdi M.; Torrez, Neil; Alizadeh, Amirali; Askari, Davood

2014-03-01

This article reports on the development of tuning fork-based AFM/STM probes in NaugaNeedles LLC for use in atomic force microscopy. These probes can be mounted on different carriers per customers' request. (e.g., RHK carrier, Omicron carrier, and tuning fork on a Sapphire disk). We are able to design and engineer tuning forks on any type of carrier used in the market. We can attach three types of tips on the edge of a tuning fork prong (i.e., growing Ag2Ga nanoneedles at any arbitrary angle, cantilever of AFM tip, and tungsten wire) with lengths from 100-500 μm. The nanoneedle is located vertical to the fork. Using a suitable insulation and metallic coating, we can make QPlus sensors that can detect tunneling current during the AFM scan. To make Qplus sensors, the entire quartz fork will be coated with an insulating material, before attaching the nanoneedle. Then, the top edge of one prong is coated with a thin layer of conductive metal and the nanoneedle is attached to the fork end of the metal coated prong. The metal coating provides electrical connection to the tip for tunneling current readout and to the electrodes and used to read the QPlus current. Since the amount of mass added to the fork is minimal, the resonance frequency spectrum does not change and still remains around 32.6 KHz and the Q factor is around 1,200 in ambient condition. These probes can enhance the performance of tuning fork based atomic microscopy.

Water quality of some logged and unlogged California streams

Treesearch

Fredric R. Kopperdahl; James W. Burns; Gary E. Smith

1971-01-01

Water quality was monitored in 1968 and 1969 in six coastal streams in northern California, four of which were subjected to logging and/or road building (Bummer Lake Creek, South Fork Yager Creek, Little North Fork Noyo River, and South Fork Caspar Creek), while the others remained undisturbed (Godwood Creek and North Fork Caspar Creek). The purposes of this study were...

Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery

PubMed Central

Murphy, Anar K.; Fitzgerald, Michael; Ro, Teresa; Kim, Jee Hyun; Rabinowitsch, Ariana I.; Chowdhury, Dipanjan; Schildkraut, Carl L.

2014-01-01

Phosphorylation of replication protein A (RPA) by Cdk2 and the checkpoint kinase ATR (ATM and Rad3 related) during replication fork stalling stabilizes the replisome, but how these modifications safeguard the fork is not understood. To address this question, we used single-molecule fiber analysis in cells expressing a phosphorylation-defective RPA2 subunit or lacking phosphatase activity toward RPA2. Deregulation of RPA phosphorylation reduced synthesis at forks both during replication stress and recovery from stress. The ability of phosphorylated RPA to stimulate fork recovery is mediated through the PALB2 tumor suppressor protein. RPA phosphorylation increased localization of PALB2 and BRCA2 to RPA-bound nuclear foci in cells experiencing replication stress. Phosphorylated RPA also stimulated recruitment of PALB2 to single-strand deoxyribonucleic acid (DNA) in a cell-free system. Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. These data demonstrate that phosphorylated RPA recruits repair factors to stalled forks, thereby enhancing fork integrity during replication stress. PMID:25113031

Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery.

PubMed

Murphy, Anar K; Fitzgerald, Michael; Ro, Teresa; Kim, Jee Hyun; Rabinowitsch, Ariana I; Chowdhury, Dipanjan; Schildkraut, Carl L; Borowiec, James A

2014-08-18

Phosphorylation of replication protein A (RPA) by Cdk2 and the checkpoint kinase ATR (ATM and Rad3 related) during replication fork stalling stabilizes the replisome, but how these modifications safeguard the fork is not understood. To address this question, we used single-molecule fiber analysis in cells expressing a phosphorylation-defective RPA2 subunit or lacking phosphatase activity toward RPA2. Deregulation of RPA phosphorylation reduced synthesis at forks both during replication stress and recovery from stress. The ability of phosphorylated RPA to stimulate fork recovery is mediated through the PALB2 tumor suppressor protein. RPA phosphorylation increased localization of PALB2 and BRCA2 to RPA-bound nuclear foci in cells experiencing replication stress. Phosphorylated RPA also stimulated recruitment of PALB2 to single-strand deoxyribonucleic acid (DNA) in a cell-free system. Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. These data demonstrate that phosphorylated RPA recruits repair factors to stalled forks, thereby enhancing fork integrity during replication stress. © 2014 Murphy et al.

Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.

PubMed

Reynolds, John J; Bicknell, Louise S; Carroll, Paula; Higgs, Martin R; Shaheen, Ranad; Murray, Jennie E; Papadopoulos, Dimitrios K; Leitch, Andrea; Murina, Olga; Tarnauskaitė, Žygimantė; Wessel, Sarah R; Zlatanou, Anastasia; Vernet, Audrey; von Kriegsheim, Alex; Mottram, Rachel M A; Logan, Clare V; Bye, Hannah; Li, Yun; Brean, Alexander; Maddirevula, Sateesh; Challis, Rachel C; Skouloudaki, Kassiani; Almoisheer, Agaadir; Alsaif, Hessa S; Amar, Ariella; Prescott, Natalie J; Bober, Michael B; Duker, Angela; Faqeih, Eissa; Seidahmed, Mohammed Zain; Al Tala, Saeed; Alswaid, Abdulrahman; Ahmed, Saleem; Al-Aama, Jumana Yousuf; Altmüller, Janine; Al Balwi, Mohammed; Brady, Angela F; Chessa, Luciana; Cox, Helen; Fischetto, Rita; Heller, Raoul; Henderson, Bertram D; Hobson, Emma; Nürnberg, Peter; Percin, E Ferda; Peron, Angela; Spaccini, Luigina; Quigley, Alan J; Thakur, Seema; Wise, Carol A; Yoon, Grace; Alnemer, Maha; Tomancak, Pavel; Yigit, Gökhan; Taylor, A Malcolm R; Reijns, Martin A M; Simpson, Michael A; Cortez, David; Alkuraya, Fowzan S; Mathew, Christopher G; Jackson, Andrew P; Stewart, Grant S

2017-04-01

To ensure efficient genome duplication, cells have evolved numerous factors that promote unperturbed DNA replication and protect, repair and restart damaged forks. Here we identify downstream neighbor of SON (DONSON) as a novel fork protection factor and report biallelic DONSON mutations in 29 individuals with microcephalic dwarfism. We demonstrate that DONSON is a replisome component that stabilizes forks during genome replication. Loss of DONSON leads to severe replication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks. Furthermore, ATM- and Rad3-related (ATR)-dependent signaling in response to replication stress is impaired in DONSON-deficient cells, resulting in decreased checkpoint activity and the potentiation of chromosomal instability. Hypomorphic mutations in DONSON substantially reduce DONSON protein levels and impair fork stability in cells from patients, consistent with defective DNA replication underlying the disease phenotype. In summary, we have identified mutations in DONSON as a common cause of microcephalic dwarfism and established DONSON as a critical replication fork protein required for mammalian DNA replication and genome stability.

Checkpoint-dependent RNR induction promotes fork restart after replicative stress.

PubMed

Morafraile, Esther C; Diffley, John F X; Tercero, José Antonio; Segurado, Mónica

2015-01-20

The checkpoint kinase Rad53 is crucial to regulate DNA replication in the presence of replicative stress. Under conditions that interfere with the progression of replication forks, Rad53 prevents Exo1-dependent fork degradation. However, although EXO1 deletion avoids fork degradation in rad53 mutants, it does not suppress their sensitivity to the ribonucleotide reductase (RNR) inhibitor hydroxyurea (HU). In this case, the inability to restart stalled forks is likely to account for the lethality of rad53 mutant cells after replication blocks. Here we show that Rad53 regulates replication restart through the checkpoint-dependent transcriptional response, and more specifically, through RNR induction. Thus, in addition to preventing fork degradation, Rad53 prevents cell death in the presence of HU by regulating RNR-expression and localization. When RNR is induced in the absence of Exo1 and RNR negative regulators, cell viability of rad53 mutants treated with HU is increased and the ability of replication forks to restart after replicative stress is restored.

Eukaryotic DNA Replication Fork.

PubMed

Burgers, Peter M J; Kunkel, Thomas A

2017-06-20

This review focuses on the biogenesis and composition of the eukaryotic DNA replication fork, with an emphasis on the enzymes that synthesize DNA and repair discontinuities on the lagging strand of the replication fork. Physical and genetic methodologies aimed at understanding these processes are discussed. The preponderance of evidence supports a model in which DNA polymerase ε (Pol ε) carries out the bulk of leading strand DNA synthesis at an undisturbed replication fork. DNA polymerases α and δ carry out the initiation of Okazaki fragment synthesis and its elongation and maturation, respectively. This review also discusses alternative proposals, including cellular processes during which alternative forks may be utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leading and lagging strand DNA synthesis separately and as an integrated replication fork.

Salmon Supplementation Studies in Idaho Rivers, 1996-1998 Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reighn, Christopher A.; Lewis, Bert; Taki, Doug

1999-06-01

Information contained in this report summarizes the work that has been done by the Shoshone-Bannock Tribes Fisheries Department under BPA Project No. 89-098-3, Contract Number 92-BI-49450. Relevant data generated by the Shoshone-Bannock Tribe will be collated with other ISS cooperator data collected from the Salmon and Clearwater rivers and tributary streams. A summary of data presented in this report and an initial project-wide level supplementation evaluation will be available in the ISS 5 year report that is currently in progress. The Shoshone-Bannock Tribal Fisheries Department is responsible for monitoring a variety of chinook salmon (Oncorhynchus tshawytscha) production parameters as partmore » of the Idaho Supplementation Studies (BPA Project No. 89-098-3, Contract Number 92-BI-49450). Parameters include parr abundance in tributaries to the upper Salmon River; adult chinook salmon spawner abundance, redd counts, and carcass collection. A rotary screw trap is operated on the East Fork Salmon River and West Fork Yankee Fork Salmon River to enumerate and PIT-tag chinook smolts. These traps are also used to monitor parr movement, and collect individuals for the State and Tribal chinook salmon captive rearing program. The SBT monitors fisheries parameters in the following six tributaries of the Salmon River: Bear Valley Creek, East Fork Salmon River, Herd Creek, South Fork Salmon River, Valley Creek, and West Fork Yankee Fork. Chinook populations in all SBT-ISS monitored streams continue to decline. The South Fork Salmon River and Bear Valley Creek have the strongest remaining populations. Snorkel survey methodology was used to obtain parr population estimates for ISS streams from 1992 to 1997. Confidence intervals for the parr population estimates were large, especially when the populations were low. In 1998, based on ISS cooperator agreement, snorkeling to obtain parr population estimates was ceased due to the large confidence intervals. A rotary screw trap was operated on the West Fork Yankee Fork during the spring, summer, and fall of 1998 to monitor juvenile chinook migration. A screw trap was also operated on the East Fork of the Salmon River during the spring and fall from 1993 to 1997. Supplementation treatments have occurred on the South Fork Salmon River (IDFG), the East Fork Salmon River (EFSR), and the West Fork Yankee Fork of the Salmon River (WFYF). The EFSR received supplementation treatments yearly through 1995. There have been no treatments since 1995, and no significant future treatments from local broodstock are planned due to extremely poor escapement. The WFYF received a single presmolt treatment in 1994. There was an egg and adult release treatment in 1998 from the captive rearing program, not part of the original ISS study. Similarly, no significant future treatments are planned for the West Fork Yankee Fork due to extremely poor escapement. However, small scale experimental captive rearing and broodstock techniques are currently being tested with populations from the EFSR and WFYF. Captive rearing/broodstock techniques could potentially provide feedback for evaluation of supplementation. The other three SBT-ISS streams are control streams and do not receive supplementation treatments.« less

Hydraulic geometry and sediment data for the South Fork Salmon River, Idaho, 1985-86

USGS Publications Warehouse

Williams, Rhea P.; O'Dell, Ivalou; Megahan, Walter F.

1989-01-01

Hydraulic geometry, suspended-sediment, and bedload samples were collected at three sites in the upper reach of the South Fork Salmon River drainage basin from April 1985 to June 1986. Sites selected were South Fork Salmon River near Krassel Ranger Station, Buckhorn Creek, and North Fork Lick Creek. Results of the data collection are presented in this report.

Rita Roars Through a Warm Gulf September 21, 2005

NASA Image and Video Library

2005-09-21

This sea surface height map of the Gulf of Mexico, with the Florida peninsula on the right and the Texas-Mexico Gulf Coast on the left, is based on altimeter data from four satellites including NASA’s Topex/Poseidon and Jason.

West Europe Report.

DTIC Science & Technology

1986-04-21

the Didacta Association, among others. Mr. Merkle , chairman of the board for Bosch, will [deliver the keynote speech] to the...concerning the Dan-F and Rolf oil fields and the Roar gas field. Incidentally, last year A.P. Miller declared to the Energy Ministry that the company

Stennis Holds Last Planned Space Shuttle Engine Test

NASA Technical Reports Server (NTRS)

2009-01-01

With 520 seconds of shake, rattle and roar on July 29, 2009 NASA's John C. Stennis Space Center marked the end of an era for testing the space shuttle main engines that have powered the nation's Space Shuttle Program for nearly three decades.

The Chip That Roared: Of Micros and Men.

ERIC Educational Resources Information Center

Licata, Christine M.; Inzinga, Joan M.

1981-01-01

The curriculum revision of accounting and office practice programs at the National Technical Institute for the Deaf at Rochester Institute of Technology through infusion of data processing skills is described. Discusses the first-year core curriculum, and second- and third-year curricula. (CT)

In-Field Performance Testing of the Fork Detector for Quantitative Spent Fuel Verification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gauld, Ian C.; Hu, Jianwei; De Baere, P.

Expanding spent fuel dry storage activities worldwide are increasing demands on safeguards authorities that perform inspections. The European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) require measurements to verify declarations when spent fuel is transferred to difficult-to-access locations, such as dry storage casks and the repositories planned in Finland and Sweden. EURATOM makes routine use of the Fork detector to obtain gross gamma and total neutron measurements during spent fuel inspections. Data analysis is performed by modules in the integrated Review and Analysis Program (iRAP) software, developed jointly by EURATOM and the IAEA. Under the frameworkmore » of the US Department of Energy–EURATOM cooperation agreement, a module for automated Fork detector data analysis has been developed by Oak Ridge National Laboratory (ORNL) using the ORIGEN code from the SCALE code system and implemented in iRAP. EURATOM and ORNL recently performed measurements on 30 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel (Clab), operated by the Swedish Nuclear Fuel and Waste Management Company (SKB). The measured assemblies represent a broad range of fuel characteristics. Neutron count rates for 15 measured pressurized water reactor assemblies are predicted with an average relative standard deviation of 4.6%, and gamma signals are predicted on average within 2.6% of the measurement. The 15 measured boiling water reactor assemblies exhibit slightly larger deviations of 5.2% for the gamma signals and 5.7% for the neutron count rates, compared to measurements. These findings suggest that with improved analysis of the measurement data, existing instruments can provide increased verification of operator declarations of the spent fuel and thereby also provide greater ability to confirm integrity of an assembly. These results support the application of the Fork detector as a fully quantitative spent fuel verification technique.« less

Regulation of Replication Fork Advance and Stability by Nucleosome Assembly

PubMed Central

Prado, Felix; Maya, Douglas

2017-01-01

The advance of replication forks to duplicate chromosomes in dividing cells requires the disassembly of nucleosomes ahead of the fork and the rapid assembly of parental and de novo histones at the newly synthesized strands behind the fork. Replication-coupled chromatin assembly provides a unique opportunity to regulate fork advance and stability. Through post-translational histone modifications and tightly regulated physical and genetic interactions between chromatin assembly factors and replisome components, chromatin assembly: (1) controls the rate of DNA synthesis and adjusts it to histone availability; (2) provides a mechanism to protect the integrity of the advancing fork; and (3) regulates the mechanisms of DNA damage tolerance in response to replication-blocking lesions. Uncoupling DNA synthesis from nucleosome assembly has deleterious effects on genome integrity and cell cycle progression and is linked to genetic diseases, cancer, and aging. PMID:28125036

Channelization and floodplain forests: impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

Treesearch

Sonja N. Oswalt; Sammy L. King

2005-01-01

We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased...

At the centre of the tuning fork

NASA Image and Video Library

2015-11-02

This galaxy is known as Mrk 820 and is classified as a lenticular galaxy — type S0 on the Hubble Tuning Fork. The Hubble Tuning Fork is used to classify galaxies according to their morphology. Elliptical galaxies look like smooth blobs in the sky and lie on the handle of the fork. They are arranged along the handle based on how elliptical they are, with the more spherical galaxies furthest from the tines of the fork, and the more egg-shaped ones closest to the end of the handle where it divides. The two prongs of the tuning fork represent types of unbarred and barred spiral galaxies. Lenticular galaxies like Mrk 820 are in the transition zone between ellipticals and spirals and lie right where the fork divides. A closer look at the appearance of Mrk 820 reveals hints of a spiral structure embedded in a circular halo of stars. Surrounding Mrk 820 in this image is good sampling of other galaxy types, covering almost every type found on the Hubble Tuning Fork, both elliptical and spiral. Most of the smears and specks are distant galaxies, but the prominent bright object at the bottom is a foreground star called TYC 4386-787-1. A version of this image was entered into the Hubble's Hidden Treasures image processing competition by contestant Judy Schmidt.

Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments.

PubMed

Kolinjivadi, Arun Mouli; Sannino, Vincenzo; De Antoni, Anna; Zadorozhny, Karina; Kilkenny, Mairi; Técher, Hervé; Baldi, Giorgio; Shen, Rong; Ciccia, Alberto; Pellegrini, Luca; Krejci, Lumir; Costanzo, Vincenzo

2017-09-07

Brca2 deficiency causes Mre11-dependent degradation of nascent DNA at stalled forks, leading to cell lethality. To understand the molecular mechanisms underlying this process, we isolated Xenopus laevis Brca2. We demonstrated that Brca2 protein prevents single-stranded DNA gap accumulation at replication fork junctions and behind them by promoting Rad51 binding to replicating DNA. Without Brca2, forks with persistent gaps are converted by Smarcal1 into reversed forks, triggering extensive Mre11-dependent nascent DNA degradation. Stable Rad51 nucleofilaments, but not RPA or Rad51 T131P mutant proteins, directly prevent Mre11-dependent DNA degradation. Mre11 inhibition instead promotes reversed fork accumulation in the absence of Brca2. Rad51 directly interacts with the Pol α N-terminal domain, promoting Pol α and δ binding to stalled replication forks. This interaction likely promotes replication fork restart and gap avoidance. These results indicate that Brca2 and Rad51 prevent formation of abnormal DNA replication intermediates, whose processing by Smarcal1 and Mre11 predisposes to genome instability. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

Co-localization of polar replication fork barriers and rRNA transcription terminators in mouse rDNA.

PubMed

López-estraño, C; Schvartzman, J B; Krimer, D B; Hernández, P

1998-03-27

We investigated the replication of the region where transcription terminates in mouse rDNA. It contains a replication fork barrier (RFB) that behaves in a polar manner, arresting only replication forks moving in the direction opposite to transcription. This RFB consists of several closely spaced fork arrest sites that co-localize with the transcription terminator elements, known as Sal boxes. Sal boxes are the target for mTTF-I (murine transcription termination factor I). These results suggest that both termination of rRNA transcription and replication fork arrest may share cis-acting as well as trans-acting factors. Copyright 1998 Academic Press Limited.

On the sound field radiated by a tuning fork

NASA Astrophysics Data System (ADS)

Russell, Daniel A.

2000-12-01

When a sounding tuning fork is brought close to the ear, and rotated about its long axis, four distinct maxima and minima are heard. However, when the same tuning fork is rotated while being held at arm's length from the ear only two maxima and minima are heard. Misconceptions concerning this phenomenon are addressed and the fundamental mode of the fork is described in terms of a linear quadrupole source. Measured directivity patterns in the near field and far field of several forks agree very well with theoretical predictions for a linear quadrupole. Other modes of vibration are shown to radiate as dipole and lateral quadrupole sources.

ATR-like kinase Mec1 facilitates both chromatin accessibility at DNA replication forks and replication fork progression during replication stress

PubMed Central

Rodriguez, Jairo; Tsukiyama, Toshio

2013-01-01

Faithful DNA replication is essential for normal cell division and differentiation. In eukaryotic cells, DNA replication takes place on chromatin. This poses the critical question as to how DNA replication can progress through chromatin, which is inhibitory to all DNA-dependent processes. Here, we developed a novel genome-wide method to measure chromatin accessibility to micrococcal nuclease (MNase) that is normalized for nucleosome density, the NCAM (normalized chromatin accessibility to MNase) assay. This method enabled us to discover that chromatin accessibility increases specifically at and ahead of DNA replication forks in normal S phase and during replication stress. We further found that Mec1, a key regulatory ATR-like kinase in the S-phase checkpoint, is required for both normal chromatin accessibility around replication forks and replication fork rate during replication stress, revealing novel functions for the kinase in replication stress response. These results suggest a possibility that Mec1 may facilitate DNA replication fork progression during replication stress by increasing chromatin accessibility around replication forks. PMID:23307868

Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities.

PubMed

Ward, T R; Hoang, M L; Prusty, R; Lau, C K; Keil, R L; Fangman, W L; Brewer, B J

2000-07-01

In the ribosomal DNA of Saccharomyces cerevisiae, sequences in the nontranscribed spacer 3' of the 35S ribosomal RNA gene are important to the polar arrest of replication forks at a site called the replication fork barrier (RFB) and also to the cis-acting, mitotic hyperrecombination site called HOT1. We have found that the RFB and HOT1 activity share some but not all of their essential sequences. Many of the mutations that reduce HOT1 recombination also decrease or eliminate fork arrest at one of two closely spaced RFB sites, RFB1 and RFB2. A simple model for the juxtaposition of RFB and HOT1 sequences is that the breakage of strands in replication forks arrested at RFB stimulates recombination. Contrary to this model, we show here that HOT1-stimulated recombination does not require the arrest of forks at the RFB. Therefore, while HOT1 activity is independent of replication fork arrest, HOT1 and RFB require some common sequences, suggesting the existence of a common trans-acting factor(s).

LOOKING EASTSOUTHEAST. Showing downstream side of completed bridge, from confluence ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOOKING EAST-SOUTHEAST. Showing downstream side of completed bridge, from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO SETTLING BASIN, SHOWING RIGHT FORK WITH GATE IN PLACE AND A FEW NEEDLES IN PLACE - Electron Hydroelectric Project, Along Puyallup River, Electron, Pierce County, WA

5. VIEW EAST ACROSS BRIDGE SHOWING RAILING DETAILS AND WATERFALLS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. VIEW EAST ACROSS BRIDGE SHOWING RAILING DETAILS AND WATERFALLS OF THE SOUTH FORK OF THE TUOLUMNE. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA

Evaluating the impacts of logging activities on erosion and suspended sediment transport in the Caspar Creek watersheds

Treesearch

Jack Lewis

1998-01-01

Suspended sediment has been sampled at both the North and South Fork weirs of Caspar Creek in northwestern California since 1963, and at 13 tributary locations in the North Fork since 1986. The North Fork gaging station (NFC) was used as a control to evaluate the effects of logging in the South Fork, in the 1970's, on annual sediment loads. In the most...

Whichever way the wind blows, scientists and engineers try to find ways to protect people and property

NASA Astrophysics Data System (ADS)

Showstack, Randy

Timothy Marshall, a failure and damage consultant with the Haag Engineering Company in Dallas, Texas, possesses a passion for storm chasing. On the afternoon of May 3, 1999, with atmospheric conditions creating a potentially explosive situation, Marshall drove several hours north to central Oklahoma to spot tornadoes. A storm started blowing up near Lawton and moved parallel to Interstate 44, with Marshall ahead of it in his Chevy pickup. He parked on the Newcastle overpass bridge, videotaping the long-tracked twister for later study At 7:04 p.m. local time, with the vortex now just one mile away and moving straight toward him, it started appearing three-dimensional, debris and projectiles flying about, the tornado roaring like freight trains, wind howling, red mud raining down, and things “getting a little out of hand,” Marshall recalled. He drove out of its path, only to watch the tornado tear through the suburban streets and houses of Moore, on its way to Oklahoma City.

A complex mechanism determines polarity of DNA replication fork arrest by the replication terminator complex of Bacillus subtilis.

PubMed

Duggin, Iain G; Matthews, Jacqueline M; Dixon, Nicholas E; Wake, R Gerry; Mackay, Joel P

2005-04-01

Two dimers of the replication terminator protein (RTP) of Bacillus subtilis bind to a chromosomal DNA terminator site to effect polar replication fork arrest. Cooperative binding of the dimers to overlapping half-sites within the terminator is essential for arrest. It was suggested previously that polarity of fork arrest is the result of the RTP dimer at the blocking (proximal) side within the complex binding very tightly and the permissive-side RTP dimer binding relatively weakly. In order to investigate this "differential binding affinity" model, we have constructed a series of mutant terminators that contain half-sites of widely different RTP binding affinities in various combinations. Although there appeared to be a correlation between binding affinity at the proximal half-site and fork arrest efficiency in vivo for some terminators, several deviated significantly from this correlation. Some terminators exhibited greatly reduced binding cooperativity (and therefore have reduced affinity at each half-site) but were highly efficient in fork arrest, whereas one terminator had normal affinity over the proximal half-site, yet had low fork arrest efficiency. The results show clearly that there is no direct correlation between the RTP binding affinity (either within the full complex or at the proximal half-site within the full complex) and the efficiency of replication fork arrest in vivo. Thus, the differential binding affinity over the proximal and distal half-sites cannot be solely responsible for functional polarity of fork arrest. Furthermore, efficient fork arrest relies on features in addition to the tight binding of RTP to terminator DNA.

South Fork Latrine, east elevation showing structure in context, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Latrine, east elevation showing structure in context, view west - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

2. VIEW OF NORTH FACE SHOWING SUBSTRUCTURE AND ABUTMENTS OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. VIEW OF NORTH FACE SHOWING SUBSTRUCTURE AND ABUTMENTS OF BRIDGE CROSSING THE SOUTH FORK OF THE TUOLUMNE RIVER. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA

Distribution and movement of bull trout in the upper Jarbidge River watershed, Nevada

USGS Publications Warehouse

Allen, M. Brady; Connolly, Patrick J.; Mesa, Matthew G.; Charrier, Jodi; Dixon, Chris

2010-01-01

In 2006 and 2007, we surveyed the occurrence of bull trout (Salvelinus confluentus), the relative distributions of bull trout and redband trout (Oncorhynchus mykiss), and stream habitat conditions in the East and West Forks of the Jarbidge River in northeastern Nevada and southern Idaho. We installed passive integrated transponder (PIT) tag interrogation systems at strategic locations within the watershed, and PIT-tagged bull trout were monitored to evaluate individual fish growth, movement, and the connectivity of bull trout between streams. Robust bull trout populations were found in the upper portions of the East Fork Jarbidge River, the West Fork Jarbidge River, and in the Pine, Jack, Dave, and Fall Creeks. Small numbers of bull trout also were found in Slide and Cougar Creeks. Bull trout were numerically dominant in the upper portions of the East Fork Jarbidge River, and in Fall, Dave, Jack, and Pine Creeks, whereas redband trout were numerically dominant throughout the rest of the watershed. The relative abundance of bull trout was notably higher at altitudes above 2,100 m. This study was successful in documenting bull trout population connectivity within the West Fork Jarbidge River, particularly between West Fork Jarbidge River and Pine Creek. Downstream movement of bull trout to the confluence of the East Fork and West Fork Jarbidge River both from Jack Creek (rkm 16.6) in the West Fork Jarbidge River and from Dave Creek (rkm 7.5) in the East Fork Jarbidge River was detected. Although bull trout exhibited some downstream movement during the spring and summer, much of their emigration occurred in the autumn, concurrent with decreasing water temperatures and slightly increasing flows. The bull trout that emigrated were mostly age-2 or older, but some age-1 fish also emigrated. Upstream movement by bull trout was detected less than downstream movement. The overall mean annual growth rate of bull trout in the East Fork and West Fork Jarbidge River was 36 mm. This growth rate is within the range reported in other river systems and is indicative of good habitat conditions. Mark-recapture methods were used to estimate a population of 147 age-1 or older bull trout in the reach of Jack Creek upstream of Jenny Creek.

A Cultural Resource Reconnaissance of Two Proposed Bank Unloading Areas, East Grand Forks, Polk County, Minnesota,

DTIC Science & Technology

1984-12-01

architectural or archeotogical evidence was identified. The southern unloading area includes the former sites of a brewery and a sawmill, both of... brewery and a sawmill, both of which were associated with 0 significant historic events and themes in the late nineteenth and early twentieth century...Forks Brewery (1888) . . . . . . . 16 Figure 4. The Grand Forks Lumber Company mill is located at A. -. Building at B is probably the East Grand Forks

Archaeological Investigations on the East Fork of the Salmon River, Custer County, Idaho.

DTIC Science & Technology

1984-01-01

coniferous environment in addition to pine marten (Martes americana), red squirrel (Tamiasciurus hudsonicus), porcupine (Erithizon dorsatum), mountain vole...can be seen in small herds throughout the East Fork valley from the Salmon River to Big Boulder Creek. Two bands of Rocky Mountain bighorn sheep...utilize the Challis Planning Unit, one on the East Fork and the other in the Birch Creek area. The East Fork herd is comprised of approximately 50-70

Dissolved-Solids Load in Henrys Fork Upstream from the Confluence with Antelope Wash, Wyoming, Water Years 1970-2009

USGS Publications Warehouse

Foster, Katharine; Kenney, Terry A.

2010-01-01

Annual dissolved-solids load at the mouth of Henrys Fork was estimated by using data from U.S. Geological Survey streamflow-gaging station 09229500, Henrys Fork near Manila, Utah. The annual dissolved-solids load for water years 1970-2009 ranged from 18,300 tons in 1977 to 123,300 tons in 1983. Annual streamflows for this period ranged from 14,100 acre-feet in 1977 to 197,500 acre-feet in 1983. The 25-percent trimmed mean dissolved-solids load for water years 1970-2009 was 44,300 tons per year at Henrys Fork near Manila, Utah. Previous simulations using a SPAtially Referenced Regression On Watershed attributes (SPARROW) model for dissolved solids specific to water year 1991 conditions in the Upper Colorado River Basin predicted an annual dissolved-solids load of 25,000 tons for the Henrys Fork Basin upstream from Antelope Wash. On the basis of computed dissolved-solids load data from Henrys Fork near Manila, Utah, together with estimated annual dissolved-solids load from Antelope Wash and Peoples Canal, this prediction was adjusted to 37,200 tons. As determined by simulations with the Upper Colorado River Basin SPARROW model, approximately 56 percent (14,000 tons per year) of the dissolved-solids load at Henrys Fork upstream from Antelope Wash is associated with the 21,500 acres of irrigated agricultural lands in the upper Henrys Fork Basin.

78 FR 42653 - Taking of Marine Mammals Incidental to Commercial Fishing Operations; Atlantic Large Whale Take...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-16

... through on their way to their Cape Cod Bay feeding ground. Recent passive acoustic studies analyzing right.... (Southern Point, Great Duck Island) 43[deg]59.36' N. lat., 68[deg]37.95' W. long. (R ``2'' Bell, Roaring...

South Fork Latrine, oblique view showing south and east sides; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Latrine, oblique view showing south and east sides; view northwest - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

South Fork Latrine showing north and west sides, general view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Latrine showing north and west sides, general view to southeast - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

COMPLETED STRUCTURE. View is eastsoutheast of downstream side of bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

COMPLETED STRUCTURE. View is east-southeast of downstream side of bridge, from beyond confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

7. View to southeast. View of downstream side of bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. View to southeast. View of downstream side of bridge from confluence of Trinity and South Fork Trinity Rivers. (90mm Lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2014 CFR

2014-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2011 CFR

2011-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2013 CFR

2013-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2012 CFR

2012-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2010 CFR

2010-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

LAKE FORK

EPA Science Inventory

The Lake Fork of the Arkansas River Watershed has been adversely affected through mining, water diversion and storage projects, grazing, logging, and other human influences over the past 120 years. It is the goals of the LFWWG to improve the health of Lake fork by addressing th...

Evaluation of the Life History of Native Salmonids in the Malheur River Basin; Cooperative Bull Trout/Redband Trout Research Project, 1999-2000 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schwabe, Lawrence; Tiley, Mark; Perkins, Raymond R.

2000-11-01

The purpose of this study is to document the seasonal distribution of adult/sub-adult bull trout (Salvelinus confluentus) in the Malheur River basin. Due to the decline of bull trout in the Columbia Basin, the United States Fish and Wildlife Service listed bull trout as a threatened species in June 1998. Past land management activities; construction of dams; and fish eradication projects in the North Fork and Middle Fork Malheur River by poisoning have worked in concert to cumulatively impact native species in the Malheur Basin (Bowers et. al. 1993). Survival of the remaining bull trout populations is severely threatened (Buchananmore » 1997). 1999 Research Objects are: (1) Document the migratory patterns of adult/sub-adult bull trout in the North Fork Malheur River; (2) Determine the seasonal bull trout use of Beulah Reservoir and bull trout entrainment; and (3) Timing and location of bull trout spawning in the North Fork Malheur River basin. The study area includes the Malheur basin from the mouth of the Malheur River located near Ontario, Oregon to the headwaters of the North Fork Malheur River (Map 1). All fish collected and most of the telemetry effort was done on the North Fork Malheur River subbasin (Map 2). Fish collection was conducted on the North Fork Malheur River at the tailwaters of Beulah Reservoir (RK 29), Beulah Reservoir (RK 29-RK 33), and in the North Fork Malheur River at Crane Crossing (RK 69) to the headwaters of the North Fork Malheur. Radio telemetry was done from the mouth of the Malheur River in Ontario, Oregon to the headwaters of the North Fork Malheur. This report will reflect all migration data collected from 3/1/99 to 12/31/99.« less

Force regulated dynamics of RPA on a DNA fork

PubMed Central

Kemmerich, Felix E.; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

2016-01-01

Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg2+ concentrations, such that human RPA can melt DNA in absence of force. PMID:27016742

South Fork Telephone Switchboard Building, oblique view of east side; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Telephone Switchboard Building, oblique view of east side; view northwest - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

South Fork Telephone Switchboard Building, interior west room showing hardwood ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Telephone Switchboard Building, interior west room showing hardwood floor; view south - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

South Fork Latrine, interior showing head with steel tank mounted ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Latrine, interior showing head with steel tank mounted to wall; view south - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

Class I Histone Deacetylase HDAC1 and WRN RECQ Helicase Contribute Additively to Protect Replication Forks upon Hydroxyurea-induced Arrest.

PubMed

Kehrli, Keffy; Phelps, Michael; Lazarchuk, Pavlo; Chen, Eleanor; Monnat, Ray; Sidorova, Julia M

2016-11-18

The WRN helicase/exonuclease is mutated in Werner syndrome of genomic instability and premature aging. WRN-depleted fibroblasts, although remaining largely viable, have a reduced capacity to maintain replication forks active during a transient hydroxyurea-induced arrest. A strand exchange protein, RAD51, is also required for replication fork maintenance, and here we show that recruitment of RAD51 to stalled forks is reduced in the absence of WRN. We performed a siRNA screen for genes that are required for viability of WRN-depleted cells after hydroxyurea treatment, and identified HDAC1, a member of the class I histone deacetylase family. One of the functions of HDAC1, which it performs together with a close homolog HDAC2, is deacetylation of new histone H4 deposited at replication forks. We show that HDAC1 depletion exacerbates defects in fork reactivation and progression after hydroxyurea treatment observed in WRN- or RAD51-deficient cells. The additive WRN, HDAC1 loss-of-function phenotype is also observed with a catalytic mutant of HDAC1; however, it does not correlate with changes in histone H4 deacetylation at replication forks. On the other hand, inhibition of histone deacetylation by an inhibitor specific to HDACs 1-3, CI-994, correlates with increased processing of newly synthesized DNA strands in hydroxyurea-stalled forks. WRN co-precipitates with HDAC1 and HDAC2. Taken together, our findings indicate that WRN interacts with HDACs 1 and 2 to facilitate activity of stalled replication forks under conditions of replication stress. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Source Process of the Mw 5.0 Au Sable Forks, New York, Earthquake Sequence from Local Aftershock Monitoring Network Data

NASA Astrophysics Data System (ADS)

Kim, W.; Seeber, L.; Armbruster, J. G.

2002-12-01

On April 20, 2002, a Mw 5 earthquake occurred near the town of Au Sable Forks, northeastern Adirondacks, New York. The quake caused moderate damage (MMI VII) around the epicentral area and it is well recorded by over 50 broadband stations in the distance ranges of 70 to 2000 km in the Eastern North America. Regional broadband waveform data are used to determine source mechanism and focal depth using moment tensor inversion technique. Source mechanism indicates predominantly thrust faulting along 45° dipping fault plane striking due South. The mainshock is followed by at least three strong aftershocks with local magnitude (ML) greater than 3 and about 70 aftershocks are detected and located in the first three months by a 12-station portable seismographic network. The aftershock distribution clearly delineate the mainshock rupture to the westerly dipping fault plane at a depth of 11 to 12 km. Preliminary analysis of the aftershock waveform data indicates that orientation of the P-axis rotated 90° from that of the mainshock, suggesting a complex source process of the earthquake sequence. We achieved an important milestone in monitoring earthquakes and evaluating their hazards through rapid cross-border (Canada-US) and cross-regional (Central US-Northeastern US) collaborative efforts. Hence, staff at Instrument Software Technology, Inc. near the epicentral area joined Lamont-Doherty staff and deployed the first portable station in the epicentral area; CERI dispatched two of their technical staff to the epicentral area with four accelerometers and a broadband seismograph; the IRIS/PASSCAL facility shipped three digital seismographs and ancillary equipment within one day of the request; the POLARIS Consortium, Canada sent a field crew of three with a near real-time, satellite telemetry based earthquake monitoring system. The Polaris station, KSVO, powered by a solar panel and batteries, was already transmitting data to the central Hub in London, Ontario, Canada within a day after the field crew arrived in the Au Sable Forks area. This collaboration allowed us to maximize the scarce resources available for monitoring this damaging earthquake and its aftershocks in the Northeastern U.S.

South Fork Telephone Switchboard Building, general view in setting showing ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Telephone Switchboard Building, general view in setting showing (N) side; view (S) - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

South Fork Telephone Switchboard Building, oblique view of (W) and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Telephone Switchboard Building, oblique view of (W) and (S) sides, view to northeast - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

STEEL ERECTION. View of downstream of bridge, looking southeast from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

STEEL ERECTION. View of downstream of bridge, looking southeast from confluence of Trinity and South Fork Trinity rivers. The old suspension bridge is in background - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe.

PubMed

Margalef, Pol; Kotsantis, Panagiotis; Borel, Valerie; Bellelli, Roberto; Panier, Stephanie; Boulton, Simon J

2018-01-25

Telomere maintenance critically depends on the distinct activities of telomerase, which adds telomeric repeats to solve the end replication problem, and RTEL1, which dismantles DNA secondary structures at telomeres to facilitate replisome progression. Here, we establish that reversed replication forks are a pathological substrate for telomerase and the source of telomere catastrophe in Rtel1 -/- cells. Inhibiting telomerase recruitment to telomeres, but not its activity, or blocking replication fork reversal through PARP1 inhibition or depleting UBC13 or ZRANB3 prevents the rapid accumulation of dysfunctional telomeres in RTEL1-deficient cells. In this context, we establish that telomerase binding to reversed replication forks inhibits telomere replication, which can be mimicked by preventing replication fork restart through depletion of RECQ1 or PARG. Our results lead us to propose that telomerase inappropriately binds to and inhibits restart of reversed replication forks within telomeres, which compromises replication and leads to critically short telomeres. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

A Rich Metadata Filesystem for Scientific Data

ERIC Educational Resources Information Center

Bui, Hoang

2012-01-01

As scientific research becomes more data intensive, there is an increasing need for scalable, reliable, and high performance storage systems. Such data repositories must provide both data archival services and rich metadata, and cleanly integrate with large scale computing resources. ROARS is a hybrid approach to distributed storage that provides…

The Mouse that Roared

ERIC Educational Resources Information Center

Venola, Penelope

2011-01-01

Seizing ideas from chance encounters and combining them in new ways produces innovative and exciting art experiences for students and teachers alike. Such was the case when the author received a book of postcards with photographs of original art made from discarded computer mice by San Francisco artists. In this article, the author describes an…

Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes.

PubMed

Hilton, Benjamin A; Liu, Ji; Cartwright, Brian M; Liu, Yiyong; Breitman, Maya; Wang, Youjie; Jones, Rowdy; Tang, Hui; Rusinol, Antonio; Musich, Phillip R; Zou, Yue

2017-09-01

Hutchinson-Gilford progeria syndrome (HGPS) is a rare genetic disorder that is caused by a point mutation in the LMNA gene, resulting in production of a truncated farnesylated-prelamin A protein (progerin). We previously reported that XPA mislocalized to the progerin-induced DNA double-strand break (DSB) sites, blocking DSB repair, which led to DSB accumulation, DNA damage responses, and early replication arrest in HGPS. In this study, the XPA mislocalization to DSBs occurred at stalled or collapsed replication forks, concurrent with a significant loss of PCNA at the forks, whereas PCNA efficiently bound to progerin. This PCNA sequestration likely exposed ds-ssDNA junctions at replication forks for XPA binding. Depletion of XPA or progerin each significantly restored PCNA at replication forks. Our results suggest that although PCNA is much more competitive than XPA in binding replication forks, PCNA sequestration by progerin may shift the equilibrium to favor XPA binding. Furthermore, we demonstrated that progerin-induced apoptosis could be rescued by XPA, suggesting that XPA-replication fork binding may prevent apoptosis in HGPS cells. Our results propose a mechanism for progerin-induced genome instability and accelerated replicative senescence in HGPS.-Hilton, B. A., Liu, J., Cartwright, B. M., Liu, Y., Breitman, M., Wang, Y., Jones, R., Tang, H., Rusinol, A., Musich, P. R., Zou, Y. Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes. © FASEB.

Base flow (1966-2009) and streamflow gain and loss (2010) of the Brazos River from the New Mexico-Texas State line to Waco, Texas

USGS Publications Warehouse

Baldys, Stanley; Schalla, Frank E.

2012-01-01

Streamflow was measured at 66 sites from June 6–9, 2010, and at 68 sites from October 16–19, 2010, to identify reaches in the upper Brazos River Basin that were gaining or losing streamflow. Gaining reaches were identified in each of the five subbasins. The gaining reach in the Salt Fork Brazos River Basin began at USGS streamflow-gaging station 08080940 Salt Fork Brazos River at State Highway 208 near Clairemont, Tex. (site SF–6), upstream from where Duck Creek flows into the Salt Fork Brazos River and continued downstream past USGS streamflow-gaging station 08082000 Salt Fork Brazos River near Aspermont, Tex. (site SF–9), to the outlet of the basin. In the Double Mountain Fork Brazos River Basin, a gaining reach from near Post, Tex., downstream to the outlet of the basin was identified. Two gaining reaches were identified in the Clear Fork Brazos River Basin—one from near Roby, Tex., downstream to near Noodle, Tex., and second from Hawley, Tex., downstream to Nugent, Tex. Most of the North Bosque River was characterized as gaining streamflow. Streamflow gains were identified in the main stem of the Brazos River from where the Brazos River main stem forms at the confluence of the Salt Fork Brazos River and Double Mountain Fork Brazos River near Knox City, Tex., downstream to near Seymour, Tex.

The effectiveness of front fork systems at damping accelerations during isolated aspects specific to cross-country mountain biking.

PubMed

Macdermid, Paul W; Miller, Matthew C; Fink, Philip W; Stannard, Stephen R

2017-11-01

Cross-country mountain bike suspension reportedly enhances comfort and performance through reduced vibration and impact exposure. This study analysed the effectiveness of three different front fork systems at damping accelerations during the crossing of three isolated obstacles (stairs, drop, and root). One participant completed three trials on six separate occasions in a randomised order using rigid, air-sprung, and carbon leaf-sprung forks. Performance was determined by time to cross obstacles, while triaxial accelerometers quantified impact exposure and damping response. Results identified significant main effect of fork type for performance time (p < 0.05). The air-sprung and leaf-sprung forks were significantly slower than the rigid forks for the stairs (p < 0.05), while air-sprung suspension was slower than the rigid for the root protocol (p < 0.05). There were no differences for the drop protocol (p < 0.05). Rigid forks reduced overall exposure (p < 0.05), specifically at the handlebars for the stairs and drop trials. More detailed analysis presented smaller vertical accelerations at the handlebar for air-sprung and leaf-sprung forks on the stairs (p < 0.05), and drop (p < 0.05) but not the root. As such, it appears that the suspension systems tested were ineffective at reducing overall impact exposure at the handlebar during isolated aspects of cross-country terrain features which may be influenced to a larger extent by rider technique.

The relationship between fat content and biological parameters of bigeye tuna ( Thunnus obseus) in the Western Central Indian Ocean

NASA Astrophysics Data System (ADS)

Song, Liming; Shen, Zhibin; Li, Jie; Li, Dongjing; Li, Yuwei

2016-10-01

Comparing fat content with physiological status can throw light on the reproduction and feeding behavior of the fish. The biological data of 586 bigeye tuna were collected from the longline fishery in the waters of Western Central Indian Ocean from November, 2012 to March, 2013. The spatial or temporal distribution of the fat content, and the relationships of fat content with gender, round weight, gonadal maturity and fork length were analyzed. A generalized additive model (GAM) was used to analyze the relationships between fat content and fork length (FL), gonadosomatic index (GSI), condition factor (K), and somatic index (SI). Results showed that: 1) the fat content of bigeye tuna was in the range of 3.1%-27.1% with the average 12.8%; 2) there were no significant geographical differences of average fat content ( P > 0.05) among 1° squares in general; 3) there were no significant differences ( P > 0.05) of the fat content for different genders, months, or gonad maturity stages; 4) there was an extremely significant correlation ( P = 0.000) between fat content and FL and GSI. There was no significant correlation ( P = 0.051) between fat content and K. There was a significant correlation ( P = 0.003) between fat content and SI. The results of this study suggest that the fat content of the matured fish was relatively stable. The survey area was in a spawning region, and the survey period was the spawning season for bigeye tuna.

An annotated bibliography of the hydrology and fishery studies of the South Fork Salmon River

Treesearch

Kathleen A. Seyedbagheri; Michael L. McHenry; William S. Platts

1987-01-01

A brief summary of the land management history of the South Fork Salmon River (Idaho) watershed includes citations and annotations of published and unpublished reports of fishery and hydrology studies conducted in the South Fork drainage for 1960 to 1986.

OLD SUSPENSION BRIDGE. This view appears to be looking southeast, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

OLD SUSPENSION BRIDGE. This view appears to be looking southeast, at the downstream side of the suspension bridge, which was replaced by the South Fork Trinity River Bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Impact. The southwest end is down, as the buckled middle ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Impact. The southwest end is down, as the buckled middle section splashes into the river. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

"Storm Alley" on Saturn and "Roaring Forties" on Earth: two bright phenomena of the same origin

NASA Astrophysics Data System (ADS)

Kochemasov, G. G.

2009-04-01

"Storm Alley" on Saturn and "Roaring Forties' on Earth: two bright phenomena of the same origin. G. Kochemasov IGEM of the Russian Academy of Sciences, Moscow, Russia, kochem.36@mail.ru Persisting swirling storms around 35 parallel of the southern latitude in the Saturnian atmosphere and famous "Roaring Forties" of the terrestrial hydro- and atmosphere are two bright phenomena that should be explained by the same physical law. The saturnian "Storm Alley" (as it is called by the Cassini scientists) is a stable feature observed also by "Voyager". The Earth's "Roaring Forties" are well known to navigators from very remote times. The wave planetology [1-3 & others] explains this similarity by a fact that both atmospheres belong to rotating globular planets. This means that the tropic and extra-tropic belts of these bodies have differing angular momenta. Belonging to one body these belts, naturally, tend to equilibrate their angular momenta mainly by redistribution of masses and densities [4]. But a perfect equilibration is impossible as long as a rotating body (Saturn or Earth or any other) keeps its globular shape due to mighty gravity. So, a contradiction of tropics and extra-tropics will be forever and the zone mainly between 30 to 50 degrees in both hemispheres always will be a zone of friction, turbulence and strong winds. Some echoes of these events will be felt farther poleward up to 70 degrees. On Earth the Roaring Forties (40˚-50˚) have a continuation in Furious Fifties (50˚-60˚) and Shrieking (Screaming) Sixties (below 60˚, close to Antarctica). Below are some examples of excited atmosphere of Saturn imaged by Cassini. PIA09734 - storms within 46˚ south; PIA09778 - monitoring the Maelstrom, 44˚ north; PIA09787 - northern storms, 59˚ north; PIA09796 - cloud details, 44˚ north; PIA10413 - storms of the high north, 70˚ north; PIA10411 - swirling storms, "Storm Alley", 35˚ south; PIA10457 - keep it rolling, "Storm Alley", 35˚ south; PIA10439 - dance of the clouds, 47˚ south; PIA10437 - dual vortices, 33˚ north. In the Earth's case the turbulence touches the atmosphere, oceans and lithosphere. Navigators for sailing use strong westerly winds in Roaring Forties. Europe is often hit by anomalous, sometimes disasters weather conditions (though winds in the northern hemisphere are somehow softened by landmasses). In the crust of Eurasia, North America and in the Southern ocean along latitudes 46˚-48˚ there are two latitudinal geomorphologic planetary flexures marking transition of subsiding inward belts to uplifting outward (pole ward) belts [5]. These slow secular crust and lithosphere movements of opposite signs witness the tendency of rotating Earth to equilibrate angular momenta of its tropic and extra-tropic belts. Thus, both planets - the rocky sphere and the gaseous giant globe - obey the same fundamental law of nature and try to adjust uneven angular momenta of its tropic and extra-tropic belts marking transition between them by anomalous features. References: [1] Kochemasov G.G. Concerted wave supergranulation of the solar system bodies // 16th Russian-American microsymposium on planetology, Abstracts, Moscow, Vernadsky Inst. (GEOKHI), 1992, 36-37. [2] Kochemasov G.G. Tectonic dichotomy, sectoring and granulation of Earth and other celestial bodies // Proceedings of the International Symposium on New Concepts in Global Tectonics, "NCGT-98 TSUKUBA", Geological Survey of Japan, Tsukuba, Nov 20-23, 1998, p. 144-147. [3] Kochemasov G.G. Theorems of wave planetary tectonics // Geophys. Res. Abstr., 1999, V.1, №3, 700. [4] Kochemasov G.G. Tectonics of rotating celestial globes // Vernadsky-Brown microsymposium 48, 20-22 Oct. 2008, Moscow, Abstr. m48_20. [5] Kotov F. S. A reflection of planetary flexures in limits of the continental lithosphere // Tectonics and geodynamics of the continental lithosphere. Proceedings of the XXXVI Tectonic conference. T. I, 4-6 Febr. 2003, Ed. Yu.V. Karyakin, Moscow, GEOS, 2003, 370 pp (p. 305-308) (In Russian).

Aftermath. The remains of the southwest end of the bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Aftermath. The remains of the southwest end of the bridge lie next to the southwest pier. View is south-southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Dust trailing from the top chord, the bridge falls toward ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Dust trailing from the top chord, the bridge falls toward the river, as the southwest end (right) falls first. View southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

With water still in midflight, the northeast end of the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

With water still in mid-flight, the northeast end of the bridge (left) nears total collapse. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

76 FR 13572 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-14

.... Specifically, it addresses the following flooding sources: Left Bank Overflow Main Stem Skagit River, Left Bank Overflow Main Stem Skagit River/South Fork Skagit River, Left Bank Overflow North Fork Skagit River, Main Stem Skagit River, North Fork Skagit River, Overflow from the Main Stem Skagit River between the North...

16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070 ...

16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070 ...

Claspin Promotes Normal Replication Fork Rates in Human Cells

PubMed Central

Helleday, Thomas; Caldecott, Keith W.

2008-01-01

The S phase-specific adaptor protein Claspin mediates the checkpoint response to replication stress by facilitating phosphorylation of Chk1 by ataxia-telangiectasia and Rad3-related (ATR). Evidence suggests that these components of the ATR pathway also play a critical role during physiological S phase. Chk1 is required for high rates of global replication fork progression, and Claspin interacts with the replication machinery and might therefore monitor normal DNA replication. Here, we have used DNA fiber labeling to investigate, for the first time, whether human Claspin is required for high rates of replication fork progression during normal S phase. We report that Claspin-depleted HeLa and HCT116 cells display levels of replication fork slowing similar to those observed in Chk1-depleted cells. This was also true in primary human 1BR3 fibroblasts, albeit to a lesser extent, suggesting that Claspin is a universal requirement for high replication fork rates in human cells. Interestingly, Claspin-depleted cells retained significant levels of Chk1 phosphorylation at both Ser317 and Ser345, raising the possibility that Claspin function during normal fork progression may extend beyond facilitating phosphorylation of either individual residue. Consistent with this possibility, depletion of Chk1 and Claspin together doubled the percentage of very slow forks, compared with depletion of either protein alone. PMID:18353973

Force regulated dynamics of RPA on a DNA fork.

PubMed

Kemmerich, Felix E; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

2016-07-08

Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg(2+) concentrations, such that human RPA can melt DNA in absence of force. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Developing a Forest Health Index for public engagement and decision support using local climatic, ecological, and socioeconomic data

NASA Astrophysics Data System (ADS)

Arnott, J. C.; Katzenberger, J.; Cundiff, J.

2013-12-01

Forest health is an oft-used term without a generally accepted definition. Nonetheless, the concept of forest health continues to permeate scientific, resource management, and public discourse, and it is viewed as a helpful communication device for engagement on issues of concern to forests and their surrounding communities. Notwithstanding the challenges associated with defining the concept of 'forest health,' we present a model for assessing forest health at a watershed scale. Utilizing the Roaring Fork Valley, Colorado--a mountain watershed of 640,000 forested acres--as a case study, we have created a Forest Health Index that integrates a range of climatic, ecological, and socioeconomic data into an assessment organized along a series of public goals including, 1) Ecosystem Services, 2) Public Health & Safety, 3) Sustainable Use & Management, and 4) Ecological Integrity. Methods for this index were adopted from an earlier effort called the Ocean Health Index by Halpern et al, 2012. Indicators that represent drivers of change, such as temperature and precipitation, as well as effects of change, such as primary productivity and phenology, were selected. Each indicator is assessed by comparing a current status of that indicator to a reference scenario obtained through one of the following methods: a) statistical analysis of baseline data from the indicator record, b) commonly accepted normals, thresholds, limits, concentrations, etc., and c) subjective expert judgment. The result of this assessment is a presentation of graphical data and accompanying ratings that combine to form an index of health for the watershed forest ecosystem. We find this product to have potential merit for communities working to assess the range of conditions affecting forest health as well as making sense of the outcomes of those affects. Here, we present a description of the index methodology, data results from engagement with forest watershed stakeholders, example results of data analysis and indexing for the index, and a synthesis of lessons learned during this two-year development process.

Effects of rainbow trout fry of a metals-contaminated diet of benthic invertebrates from the Clark Fork River, Montana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Woodward, D.F.; Brumbaugh, W.G.; DeLonay, A.J.

1994-01-01

The upper Clark Fork River in northwestern Montana has received mining wastes from the Butte and Anaconda areas since 1880. These wastes have contaminated areas of the river bed and floodplain with tailings and heavy metal sludge, resulting in elevated concentration of metals in surface water, sediments, and biota. Rainbow trout Oncorhynchus mykiss were exposed immediately after hatching for 91 d to cadmium, copper, lead, and zinc in water at concentrations simulating those in Clark Fork River. From exogenous feeding (21 d posthatch) through 91 d, fry were also fed benthic invertebrates from the Clark Fork River that contained elevatedmore » concentrations of arsenic, cadmium, copper, and lead. Evaluations of different combinations of diet and water exposure indicated diet-borne metals were more important than water-borne metals - at the concentrations we tested - in reducing survival and growth of rainbow trout. Whole-body metal concentrations ([mu]g/g, wet weight) at 91 d in fish fed Clark Fork invertebrates without exposure to Clark Fork water were arsenic, 1.4; cadmium, 0.16; and copper, 6.7. These were similar to concentrations found in Clark Fork River fishes. Livers from fish on the high-metals diets exhibited degenerative changes and generally lacked glycogen vacuolation. Indigenous Clark Fork River invertebrates provide a concentrated source of metals for accumulation into young fishes, and probably were the cause of decreased survival and growth of age-0 rainbow trout in our laboratory exposures. 30 refs., 8 figs., 4 tabs.« less

76 FR 53082 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-25

..., 201 Saint Andrews Street, Tarboro, NC 27886. Smith County, Texas, and Incorporated Areas Black Fork.... Tributary BF-1 At the Black Fork Creek +434 +436 City of Tyler, confluence. Unincorporated Areas of Smith County. Approximately 1.2 miles None +476 upstream of Loop 323. Tributary BF-M-1 At the Black Fork Creek...

11. DETAIL VIEW OF BRIDGE DATEPLATE AT SOUTHEAST CORNER OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. DETAIL VIEW OF BRIDGE DATEPLATE AT SOUTHEAST CORNER OF BRIDGE WHICH READS 'NORTH FORK OF WHITE RIVER, VINCENNES STEEL CORP., CONTRACTOR, ARKANSAS STATE HIGHWAY COMMISSION AND THE UNITED STATES BUREAU OF PUBLIC ROADS, 1936' - North Fork Bridge, Spans North Fork of White River at State Highway 5, Norfork, Baxter County, AR

With deck removed and critical members severed, the bridge begins ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

With deck removed and critical members severed, the bridge begins to succumb to tractors, pulling cables attached to its downstream side. View is to the southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Spray and dust hang in the air, as the demolition ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Spray and dust hang in the air, as the demolition is completed. Only the southeast pier remains standing at right. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

76 FR 70397 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-14

... the Ohio River +549 +550 Town of Fort Gay, Unincorporated Areas of Wayne County. confluence. At the... of Fort Gay. Fork). confluence to approximately 1.1 miles upstream of the Tug Fork confluence. Tug Fork At the Big Sandy River +576 +575 Town of Fort Gay. confluence. Approximately 0.5 mile +577 +575...

Integrating Salmon Recovery, Clean Water Act Compliance, Restoration, and Climate Change Impacts in the South Fork Nooksack River

EPA Science Inventory

"The South Fork Nooksack River (SFNR) is an important tributary to the Nooksack River, Bellingham Bay, and the Salish Sea. The South Fork Nooksack River comprises one of the 22 independent populations of spring Chinook in the Puget Sound Chinook Evolutionarily Significant Un...

76 FR 46721 - Salmon-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-03

...-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project Environmental Impact... improve the health of the ecosystem and reach the desired future condition. DATES: Comments concerning the... Ecosystem Restoration Project EIS, P.O. Box 180, 11 Casey Rd., North Fork, ID 83466. Comments may also be...

75 FR 57493 - Notice of Inventory Completion: Department of Anthropology & Ethnic Studies, University of Nevada...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-21

..., Nevada; Shoshone-Paiute Tribes of the Duck Valley Reservation, Nevada; South Fork Band (Constituent Band... of Idaho; Shoshone-Paiute Tribes of the Duck Valley Reservation, Nevada; South Fork Band (Constituent... Idaho; Shoshone-Paiute Tribes of the Duck Valley Reservation, Nevada; South Fork Band; Summit Lake...

76 FR 35009 - Draft Oil and Gas Management Plan/Environmental Impact Statement for Big South Fork National...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-15

... DEPARTMENT OF THE INTERIOR National Park Service [5130-0400-NZM] Draft Oil and Gas Management Plan... Management Plan/ Environmental Impact Statement for Big South Fork National River and Recreation Area and... gas management plan/environmental impact statement (OGMP/DEIS) for the proposed Big South Fork...

Training Guidelines: Fork Lift Truck Driving.

ERIC Educational Resources Information Center

Ceramics, Glass, and Mineral Products Industry Training Board, Harrow (England).

This manual of operative training guidelines for fork lift truck driving has been developed by the Ceramics, Glass and Mineral Products Industry Training Board (Great Britain) in consultation with a number of firms which manufacture fork lift trucks or which already have training--programs for their use. The purpose of the guidelines is to assist…

HOWARD FORK ACID ROCK DRAINAGE SOURCE INTERCEPTION STUDY; HOWARD FORK OF THE SAN MIGUEL RIVER NEAR OPHIR, COLORADO

EPA Science Inventory

This project proposes to analyze regional hydrogeology as it relates to mine workings which discharge significant heavy metals into the Howard Fork of the San Miguel River and recommend strategies to intercept and divert water away from mineralized zones. The study also includes...

Chk1 promotes replication fork progression by controlling replication initiation

PubMed Central

Petermann, Eva; Woodcock, Mick; Helleday, Thomas

2010-01-01

DNA replication starts at initiation sites termed replication origins. Metazoan cells contain many more potential origins than are activated (fired) during each S phase. Origin activation is controlled by the ATR checkpoint kinase and its downstream effector kinase Chk1, which suppresses origin firing in response to replication blocks and during normal S phase by inhibiting the cyclin-dependent kinase Cdk2. In addition to increased origin activation, cells deficient in Chk1 activity display reduced rates of replication fork progression. Here we investigate the causal relationship between increased origin firing and reduced replication fork progression. We use the Cdk inhibitor roscovitine or RNAi depletion of Cdc7 to inhibit origin firing in Chk1-inhibited or RNAi-depleted cells. We report that Cdk inhibition and depletion of Cdc7 can alleviate the slow replication fork speeds in Chk1-deficient cells. Our data suggest that increased replication initiation leads to slow replication fork progression and that Chk1 promotes replication fork progression during normal S phase by controlling replication origin activity. PMID:20805465

Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation

NASA Astrophysics Data System (ADS)

Min, Wookee; Bruhn, Christopher; Grigaravicius, Paulius; Zhou, Zhong-Wei; Li, Fu; Krüger, Anja; Siddeek, Bénazir; Greulich, Karl-Otto; Popp, Oliver; Meisezahl, Chris; Calkhoven, Cornelis F.; Bürkle, Alexander; Xu, Xingzhi; Wang, Zhao-Qi

2013-12-01

Damaged replication forks activate poly(ADP-ribose) polymerase 1 (PARP1), which catalyses poly(ADP-ribose) (PAR) formation; however, how PARP1 or poly(ADP-ribosyl)ation is involved in the S-phase checkpoint is unknown. Here we show that PAR, supplied by PARP1, interacts with Chk1 via a novel PAR-binding regulatory (PbR) motif in Chk1, independent of ATR and its activity. iPOND studies reveal that Chk1 associates readily with the unperturbed replication fork and that PAR is required for efficient retention of Chk1 and phosphorylated Chk1 at the fork. A PbR mutation, which disrupts PAR binding, but not the interaction with its partners Claspin or BRCA1, impairs Chk1 and the S-phase checkpoint activation, and mirrors Chk1 knockdown-induced hypersensitivity to fork poisoning. We find that long chains, but not short chains, of PAR stimulate Chk1 kinase activity. Collectively, we disclose a previously unrecognized mechanism of the S-phase checkpoint by PAR metabolism that modulates Chk1 activity at the replication fork.

Role of replication protein A as sensor in activation of the S-phase checkpoint in Xenopus egg extracts

PubMed Central

Recolin, Bénédicte; Van Der Laan, Siem; Maiorano, Domenico

2012-01-01

Uncoupling between DNA polymerases and helicase activities at replication forks, induced by diverse DNA lesions or replication inhibitors, generate long stretches of primed single-stranded DNA that is implicated in activation of the S-phase checkpoint. It is currently unclear whether nucleation of the essential replication factor RPA onto this substrate stimulates the ATR-dependent checkpoint response independently of its role in DNA synthesis. Using Xenopus egg extracts to investigate the role of RPA recruitment at uncoupled forks in checkpoint activation we have surprisingly found that in conditions in which DNA synthesis occurs, RPA accumulation at forks stalled by either replication stress or UV irradiation is dispensable for Chk1 phosphorylation. In contrast, when both replication fork uncoupling and RPA hyperloading are suppressed, Chk1 phosphorylation is inhibited. Moreover, we show that extracts containing reduced levels of RPA accumulate ssDNA and induce spontaneous, caffeine-sensitive, Chk1 phosphorylation in S-phase. These results strongly suggest that disturbance of enzymatic activities of replication forks, rather than RPA hyperloading at stalled forks, is a critical determinant of ATR activation. PMID:22187152

The Study of World History: Triolets, Villanelles, and Roaring in the Pines.

ERIC Educational Resources Information Center

Roselle, Daniel

1992-01-01

Principal theme in world history should be universality of basic human experience. This approach values differing forms of human expression without associating abstract concepts (such as faith, reason, or creativity) to one specific period of history in one particular part of the world. Scholarship should remain the highest priority, and past…

Not Hoovervilles, but Hooch: Gertrude Chandler Warner's "The Boxcar Children" and the Roaring Twenties

ERIC Educational Resources Information Center

Abate, Michelle Ann

2016-01-01

This essay provides much-needed critical attention and historical context to the long-neglected 1924 edition of Gertrude Chandler Warner's "The Box-Car Children." Commonly overshadowed by its more recent and more popular 1942 version--known as "The Boxcar Children"--this earlier edition calls attention to the original cultural…

More Rip-Roaring Reads for Reluctant Teen Readers.

ERIC Educational Resources Information Center

Ammon, Bette D.; Sherman, Gale W.

This publication describes 40 fiction and nonfiction titles for grades 5 through 12 that will get the attention of even the most reluctant readers. Books range from humor and romance to adventure, mystery, and the supernatural. The titles were chosen based on the following criteria: recent publication date; relatively short length; appealing…

Learning to Roar

ERIC Educational Resources Information Center

Cowhey, Mary

2010-01-01

In K-12 education, there is a saying. How can teachers close the "achievement gap"? In other words, if the students' parents speak little or no English, often lack formal education, and are strained by poor health, violence, inadequate housing and transportation, and the stresses of living in poverty, how can teachers be expected to bring those…

From a Roar to a Murmur: Virginia's History & Social Science Standards, 1995-2009

ERIC Educational Resources Information Center

van Hover, Stephanie; Hicks, David; Stoddard, Jeremy; Lisanti, Melissa

2010-01-01

The authors trace the development and implementation of Virginia's History and Social Science standards-based accountability system from 1995 to 2009. They frame the study within an examination of the political ideologies that influence policy realization and unpack the relationship between ideological and epistemological beliefs about the nature…

Blended and Co-Existing Worlds in Intersectoral Mobilities of European PhD Graduates in the Social Sciences and Humanities

ERIC Educational Resources Information Center

Millard, Debbie

2018-01-01

This paper argues that links between academia and other economic sectors are increasing, especially through intersectoral mobility of university graduates. Murray [(2010). "The Oncomouse that Roared: Hybrid Exchange Strategies as a Source of Distinction at the Boundary of Overlapping Institutions." "American Journal of…

Blended Instruction: The Roaring Twenties Meets Coursesites.com

ERIC Educational Resources Information Center

Waldron, Diane L.

2014-01-01

The action research study described in this report outlines the design and implementation of a unit of blended instruction in a traditional high school English classroom. Twenty technical high school students in an 11th grade Honors English class engaged in a variety of internet-based activities in conjunction with traditional learning activities…

The Tunebook That Roars: The Sound and Style of Sacred Harp Singing.

ERIC Educational Resources Information Center

Scholten, James

1980-01-01

The Chattahoochee, Georgia, Sacred Harp Musical Convention has been held annually since 1852. Its tunebook uses a shape-note solmization system introduced to America in the seventeenth century. This article describes the history of shape-note tunebooks; some Sacred Harp songs, modern singing events, and teaching techniques. Resources are listed.…

Water-Quality, Bed-Sediment, and Biological Data (October 2007 through September 2008) and Statistical Summaries of Long-Term Data for Streams in the Clark Fork Basin, Montana

USGS Publications Warehouse

Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

2009-01-01

Water, bed sediment, and biota were sampled in streams from Butte to near Missoula as part of a long-term monitoring program in the upper Clark Fork basin; additional water samples were collected in the Clark Fork basin from sites near Missoula downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water samples were collected periodically at 23 sites from October 2007 through September 2008. Bed-sediment and biota samples were collected once at 13 sites during August 2008. This report presents the analytical results and quality assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2007 through September 2008. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Turbidity was analyzed for water samples collected at sites where seasonal daily values of turbidity were being determined and at Clark Fork above Missoula. Nutrients also were analyzed at all the supplemental water-quality sites, except for Clark Fork Bypass, near Bonner. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for four sites, and seasonal daily values of turbidity were determined for four sites. Bed-sediment data include trace-element concentrations in the fine-grained fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

Water-quality, bed-sediment, and biological data (October 2008 through September 2009) and statistical summaries of long-term data for streams in the Clark Fork basin, Montana

USGS Publications Warehouse

Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

2010-01-01

Water, bed sediment, and biota were sampled in streams from Butte to near Missoula, Montana, as part of a long-term monitoring program in the upper Clark Fork basin; additional water samples were collected in the Clark Fork basin from sites near Missoula downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted by the U.S. Geological Survey in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water samples were collected periodically at 24 sites from October 2008 through September 2009. Bed-sediment and biota samples were collected once at 13 sites during August 2009. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2008 through September 2009. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Turbidity was analyzed for water samples collected at the four sites where seasonal daily values of turbidity were being determined as well as at Clark Fork above Missoula. Nutrients also were analyzed at all the supplemental water-quality sites, except for Clark Fork Bypass, near Bonner. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for four sites. Bed-sediment data include trace-element concentrations in the fine-grained fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

Whistler Waves With Electron Temperature Anisotropy And Non-Maxwellian Distribution Functions

NASA Astrophysics Data System (ADS)

Masood, W.

2017-12-01

Low frequency waves (˜ 100Hz), popularly known as Lion roars, are ubiquitously observed by satellites in terrestrial magnetosheath. By dint of both wave and electron data from the Cluster spacecraft and employing the linear kinetic theory for the electromagnetic waves, Masood et. al. (Ann. Geophysicae. 24, 1725-1735 (2006)) examined the conjecture made by Thorne and Tsurutani (Nature, 93, 384 (1981)) that whistler waves with electron temperature anisotropy are the progenitors of lion roars. It turned out that the study based upon the bi-Maxwellian distribution function did not come up with a satisfactory explanation of certain disagreements between theory and data. In this paper, we revisit the problem using the generalized (r, q) distribution to carry out the linear stability analysis. It is shown that good qualitative and quantitative agreements are found between theory and data using this distribution. Whistler waves with electron temperature anisotropy are also investigated with other non-Maxwellian distribution functions and general comparison is made in the end and differences in each case are highlighted. The possible applications in space plasmas are also pointed out.

Water quality and relation to taste-and-odor compounds in North Fork Ninnescah River and Cheney Reservoir, south-central Kansas, 1997-2003

USGS Publications Warehouse

Christensen, Victoria G.; Graham, Jennifer L.; Milligan, Chad R.; Pope, Larry M.; Ziegler, Andrew C.

2006-01-01

Regression models were developed between geosmin and the physical property measurements continuously recorded by water-quality monitors at each site. The geosmin regression model was applied to water-quality monitor measurements, providing a continuous estimate of geosmin for 2003. The city of Wichita will be able to use this type of analysis to determine the probability of when concentrations of geosmin are likely to be at or above the human detection level of 0.01 microgram per liter.

Proceedings of the ninetieth stated meeting of the American Ornithologists' Union

USGS Publications Warehouse

Banks, R.C.

1973-01-01

The Ninetieth Stated Meeting of the American Ornithologists' Union was held 14-18 August 1972 at Grand Forks, North Dakota, under the sponsorship of the University of North Dakota and the North Dakota Natural Science Society. Business and technical sessions were held on the campus of the University; some social activities were held at the Westward Ho Motel. Daylong field trips were taken to Lake Itasca, Minnesota, State Park and to saline lakes and prairie potholes in central North Dakota. A longer post-meeting trip to western North Dakota was offered.

Geologic map of the Dillon quadrangle, Summit and Grand Counties, Colorado

USGS Publications Warehouse

Kellogg, Karl S.

2002-01-01

New 1:24,000-scale geologic mapping along the Interstate-70 urban corridor in western Colorado, in support of the USGS Central Region State/USGS Cooperative Geologic Mapping Project, is contributing to a more complete understanding of the stratigraphy, structure, tectonic evolution, and hazard potential of this rapidly developing region. The 1:24,000-scale Dillon quadrangle is near the headwaters of the Blue River and straddles features of the Blue River graben (Kellogg, 1999), part of the northernmost reaches of the Rio Grande rift, a major late Oligocene to recent zone of extension that extends from Colorado to Mexico. The Williams Range thrust fault, the western structural margin of the Colorado Front Range, cuts through the center of the quadrangle, although is mostly covered by surficial deposits. The oldest rocks in the quadrangle underlie the Williams Fork Mountains and the ridge immediately east of South Fork Middle Fork River, and include biotite-sillimanite schist and gneiss, amphibolite, and migmatite that are intruded by granite inferred to be part of the 1,667-1,750 Ma Routt Plutonic Suite (Tweto, 1987). The oldest exposed sedimentary unit is the Upper Jurassic Morrison Formation, but Pennsylvanian Maroon Formation, a sequence of red sandstone, conglomerate, and interbedded shale, underlies the southern part of the quadrangle. The thickest sequence of sedimentary rocks is Cretaceous in age and includes at least 500 m of the Upper Cretaceous Pierre Shale. Surficial deposits include (1) an old, deeply dissected landslide deposit, possibly as old as Pliocene, on the west flank of the Williams Fork Mountains, (2) deeply weathered, very coarse gravel deposits underlying a mesa in the southwest part of the quadrangle (the Mesa Cortina subdivision. The gravels are gold bearing and were mined by hydraulic methods in the 1800s), (3) moderately to deeply weathered, widespread, bouldery material that is a combination of till of the Bull Lake glaciation, debris-flow deposits, landslide deposits, and possibly pre-Bull Lake till, (4) glacial deposits of both Bull Lake (middle Pleistocene) and Pinedale (late Pleistocene)glaciations, (5) recent landslide deposits, and (6)extensive colluvial and alluvial deposits.

Geologic map of the Dillon quadrangle, Summit and Grand Counties, Colorado

USGS Publications Warehouse

Kellogg, Karl S.

1997-01-01

New 1:24,000-scale geologic mapping along the Interstate-70 urban corridor in western Colorado, in support of the USGS Central Region State/USGS Cooperative Geologic Mapping Project, is contributing to a more complete understanding of the stratigraphy, structure, tectonic evolution, and hazard potential of this rapidly developing region. The 1:24,000-scale Dillon quadrangle is near the headwaters of the Blue River and straddles features of the Blue River graben (Kellogg, 1999), part of the northernmost reaches of the Rio Grande rift, a major late Oligocene to recent zone of extension that extends from Colorado to Mexico. The Williams Range thrust fault, the western structural margin of the Colorado Front Range, cuts through the center of the quadrangle, although is mostly covered by surficial deposits. The oldest rocks in the quadrangle underlie the Williams Fork Mountains and the ridge immediately east of South Fork Middle Fork River, and include biotite-sillimanite schist and gneiss, amphibolite, and migmatite that are intruded by granite inferred to be part of the 1,667-1,750 Ma Routt Plutonic Suite (Tweto, 1987). The oldest exposed sedimentary unit is the Upper Jurassic Morrison Formation, but Pennsylvanian Maroon Formation, a sequence of red sandstone, conglomerate, and interbedded shale, underlies the southern part of the quadrangle. The thickest sequence of sedimentary rocks is Cretaceous in age and includes at least 500 m of the Upper Cretaceous Pierre Shale. Surficial deposits include (1) an old, deeply dissected landslide deposit, possibly as old as Pliocene, on the west flank of the Williams Fork Mountains, (2) deeply weathered, very coarse gravel deposits underlying a mesa in the southwest part of the quadrangle (the Mesa Cortina subdivision. The gravels are gold bearing and were mined by hydraulic methods in the 1800s), (3) moderately to deeply weathered, widespread, bouldery material that is a combination of till of the Bull Lake glaciation, debris-flow deposits, landslide deposits, and possibly pre-Bull Lake till, (4) glacial deposits of both Bull Lake (middle Pleistocene) and Pinedale (late Pleistocene)glaciations, (5) recent landslide deposits, and (6)extensive colluvial and alluvial deposits.

The Human RNA Polymerase I Transcription Terminator Complex Acts as a Replication Fork Barrier That Coordinates the Progress of Replication with rRNA Transcription Activity.

PubMed

Akamatsu, Yufuko; Kobayashi, Takehiko

2015-05-01

In S phase, the replication and transcription of genomic DNA need to accommodate each other, otherwise their machineries collide, with chromosomal instability as a possible consequence. Here, we characterized the human replication fork barrier (RFB) that is present downstream from the 47S pre-rRNA gene (ribosomal DNA [rDNA]). We found that the most proximal transcription terminator, Sal box T1, acts as a polar RFB, while the other, Sal box T4/T5, arrests replication forks bidirectionally. The fork-arresting activity at these sites depends on polymerase I (Pol I) transcription termination factor 1 (TTF-1) and a replisome component, TIMELESS (TIM). We also found that the RFB activity was linked to rDNA copies with hypomethylated CpG and coincided with the time that actively transcribed rRNA genes are replicated. Failed fork arrest at RFB sites led to a slowdown of fork progression moving in the opposite direction to rRNA transcription. Chemical inhibition of transcription counteracted this deceleration of forks, indicating that rRNA transcription impedes replication in the absence of RFB activity. Thus, our results reveal a role of RFB for coordinating the progression of replication and transcription activity in highly transcribed rRNA genes. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

76 FR 34690 - Placer County Water Agency; Notice of Application Accepted for Filing, Soliciting Motions To...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-14

... Water Agency; Notice of Application Accepted for Filing, Soliciting Motions To Intervene and Protests... Water Agency. e. Name of Project: Middle Fork American River Project. f. Location: The Middle Fork....S. Department of Agriculture-- Forest Service. g. Filed Pursuant to: Federal Power Act 16 U.S.C. 791...

Qualitative Assessment: Evaluating the Impacts of Climate Change on Endangered Species Act Recovery Actions for the South Fork Nooksack River, WA

EPA Science Inventory

The South Fork Nooksack River (South Fork) is located in northwest Washington State and is home to nine species of Pacific salmon, including Nooksack early Chinook (aka, spring Chinook salmon), an iconic species for the Nooksack Indian Tribe. The quantity of salmon in the South F...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-20

... Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the National... Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park Service... services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not to exceed 3...

9. 'CRIB DAM IN LAKE FORK RIVER AT HEADING OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. 'CRIB DAM IN LAKE FORK RIVER AT HEADING OF LAKE FORK CANAL, UINTAH PROJECT. TWO SLUICEWAYS TWENTY FEET WIDE HAVE BEEN LEFT IN THE DAM TO PASS BOULDERS DURING HIGH WATER. THESE SLUICEWAYS ARE CLOSED BY LOGS AND HAY DURING LOW WATER.' Date unknown - Irrigation Canals in the Uinta Basin, Duchesne, Duchesne County, UT

16 CFR 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Requirements for fork and frame assembly... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

16 CFR 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Requirements for fork and frame assembly... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

16 CFR 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for fork and frame assembly... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

16 CFR 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Requirements for fork and frame assembly... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

Utilization of protein expression profiles as indicators of environmental impairment of smallmouth bass (Micropterus dolomieu) from the Shenandoah River, Virginia, USA.

PubMed

Ripley, Jennifer; Iwanowicz, Luke; Blazer, Vicki; Foran, Christy

2008-08-01

The Shenandoah River (VA, USA), the largest tributary of the Potomac River (MD, USA) and an important source of drinking water, has been the site of extensive fish kills since 2004. Previous investigations indicate environmental stressors may be adversely modulating the immune system of smallmouth bass (Micropterus dolomieu) and other species. Anterior kidney (AK) tissue, the major site of blood cell production in fish, was collected from smallmouth bass at three sites along the Shenandoah River. The tissue was divided for immune function and proteomics analyses. Bactericidal activity and respiratory burst were significantly different between North Fork and mainstem Shenandoah River smallmouth bass, whereas South Fork AK tissue did not significantly differ in either of these measures compared with the other sites. Cytotoxic cell activity was highest among South Fork and lowest among North Fork AK leukocytes. The composite two-dimension gels of the North Fork and mainstem smallmouth bass AK tissues contained 584 and 591 spots, respectively. South Fork smallmouth bass AK expressed only 335 proteins. Nineteen of 50 proteins analyzed by matrix-assisted laser desorption ionization-time of flight were successfully identified. Three of the four identified proteins with increased expression in South Fork AK tissue were involved in metabolism. Seven proteins exclusive to mainstem and North Fork smallmouth bass AK and expressed at comparable abundances serve immune and stress response functions. The proteomics data indicate these fish differ in metabolic capacity of AK tissue and in the ability to produce functional leukocytes. The variable responses of the immune function assays further indicate disruption to the immune system. Our results allow us to hypothesize underlying physiological changes that may relate to fish kills and suggest relevant contaminants known to produce similar physiological disruption.

Utilization of protein expression profiles as indicators of environmental impairment of smallmouth bass (Micropterus dolomieu) from the Shenandoah River, Virginia, USA

USGS Publications Warehouse

Ripley, J.; Iwanowicz, L.; Blazer, V.; Foran, C.

2008-01-01

The Shenandoah River (VA, USA), the largest tributary of the Potomac River (MD, USA) and an important source of drinking water, has been the site of extensive fish kills since 2004. Previous investigations indicate environmental stressors may be adversely modulating the immune system of smallmouth bass (Micropterus dolomieu) and other species. Anterior kidney (AK) tissue, the major site of blood cell production in fish, was collected from smallmouth bass at three sites along the Shenandoah River. The tissue was divided for immune function and proteomics analyses. Bactericidal activity and respiratory burst were significantly different between North Fork and mainstem Shenandoah River smallmouth bass, whereas South Fork AK tissue did not significantly differ in either of these measures compared with the other sites. Cytotoxic cell activity was highest among South Fork and lowest among North Fork AK leukocytes. The composite two-dimension gels of the North Fork and mainstem smallmouth bass AK tissues contained 584 and 591 spots, respectively. South Fork smallmouth bass AK expressed only 335 proteins. Nineteen of 50 proteins analyzed by matrix-assisted laser desorption ionization-time of flight were successfully identified. Three of the four identified proteins with increased expression in South Fork AK tissue were involved in metabolism. Seven proteins exclusive to mainstem and North Fork smallmouth bass AK and expressed at comparable abundances serve immune and stress response functions. The proteomics data indicate these fish differ in metabolic capacity of AK tissue and in the ability to produce functional leukocytes. The variable responses of the immune function assays further indicate disruption to the immune system. Our results allow us to hypothesize underlying physiological changes that may relate to fish kills and suggest relevant contaminants known to produce similar physiological disruption. ?? 2008 SETAC.

U.S. Geological Survey 2013 assessment of undiscovered resources in the Bakken and Three Forks Formations of the U.S. Williston Basin Province

USGS Publications Warehouse

Gaswirth, Stephanie B.; Marra, Kristen R.

2014-01-01

The Upper Devonian Three Forks and Upper Devonian to Lower Mississippian Bakken Formations comprise a major United States continuous oil resource. Current exploitation of oil is from horizontal drilling and hydraulic fracturing of the Middle Member of the Bakken and upper Three Forks, with ongoing exploration of the lower Three Forks, and the Upper, Lower, and Pronghorn Members of the Bakken Formation. In 2008, the U.S. Geological Survey (USGS) estimated a mean of 3.65 billion bbl of undiscovered, technically recoverable oil resource within the Bakken Formation. The USGS recently reassessed the Bakken Formation, which included an assessment of the underlying Three Forks Formation. The Pronghorn Member of the Bakken Formation, where present, was included as part of the Three Forks assessment due to probable fluid communication between reservoirs. For the Bakken Formation, five continuous and one conventional assessment units (AUs) were defined. These AUs are modified from the 2008 AU boundaries to incorporate expanded geologic and production information. The Three Forks Formation was defined with one continuous and one conventional AU. Within the continuous AUs, optimal regions of hydrocarbon recovery, or “sweet spots,” were delineated and estimated ultimate recoveries were calculated for each continuous AU. Resulting undiscovered, technically recoverable resource estimates were 3.65 billion bbl for the five Bakken continuous oil AUs and 3.73 billion bbl for the Three Forks Continuous Oil AU, generating a total mean resource estimate of 7.38 billion bbl. The two conventional AUs are hypothetical and represent a negligible component of the total estimated resource (8 million barrels of oil).

A Galaxy at the Center of the Hubble Tuning Fork

NASA Image and Video Library

2017-12-08

This galaxy is known as Mrk 820 and is classified as a lenticular galaxy — type S0 on the Hubble Tuning Fork. The Hubble Tuning Fork is used to classify galaxies according to their morphology. Elliptical galaxies look like smooth blobs in the sky and lie on the handle of the fork. They are arranged along the handle based on how elliptical they are, with the more spherical galaxies furthest from the tines of the fork, and the more egg-shaped ones closest to the end of the handle where it divides. The two prongs of the tuning fork represent types of unbarred and barred spiral galaxies. Lenticular galaxies like Mrk 820 are in the transition zone between ellipticals and spirals and lie right where the fork divides. A closer look at the appearance of Mrk 820 reveals hints of a spiral structure embedded in a circular halo of stars. Surrounding Mrk 820 in this image is a good sampling of other galaxy types, covering almost every type found on the Hubble Tuning Fork, both elliptical and spiral. Most of the smears and specks are distant galaxies, but the prominent bright object at the bottom is a foreground star called TYC 4386-787-1. Credit: ESA/Hubble & NASA and N. Gorin (STScI), Acknowledgement: Judy Schmidt NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Tuning fork enhanced interferometric photoacoustic spectroscopy: a new method for trace gas analysis

NASA Astrophysics Data System (ADS)

Köhring, M.; Pohlkötter, A.; Willer, U.; Angelmahr, M.; Schade, W.

2011-01-01

A photoacoustic trace gas sensor based on an optical read-out method of a quartz tuning fork is shown. Instead of conventional piezoelectric signal read-out, as applied in well-known quartz-enhanced photoacoustic spectroscopy (QEPAS), an interferometric read-out method for measurement of the tuning fork's oscillation is presented. To demonstrate the potential of the optical read-out of tuning forks in photoacoustics, a comparison between the performances of a sensor with interferometric read-out and conventional QEPAS with piezoelectric read-out is reported. The two sensors show similar characteristics. The detection limit (L) for the optical read-out is determined to be L opt=(2598±84) ppm (1 σ) compared to L elec=(2579±78) ppm (1 σ) for piezoelectric read-out. In both cases the detection limit is defined by the thermal noise of the tuning fork.

ATR prohibits replication catastrophe by preventing global exhaustion of RPA.

PubMed

Toledo, Luis Ignacio; Altmeyer, Matthias; Rask, Maj-Britt; Lukas, Claudia; Larsen, Dorthe Helena; Povlsen, Lou Klitgaard; Bekker-Jensen, Simon; Mailand, Niels; Bartek, Jiri; Lukas, Jiri

2013-11-21

ATR, activated by replication stress, protects replication forks locally and suppresses origin firing globally. Here, we show that these functions of ATR are mechanistically coupled. Although initially stable, stalled forks in ATR-deficient cells undergo nucleus-wide breakage after unscheduled origin firing generates an excess of single-stranded DNA that exhausts the nuclear pool of RPA. Partial reduction of RPA accelerated fork breakage, and forced elevation of RPA was sufficient to delay such "replication catastrophe" even in the absence of ATR activity. Conversely, unscheduled origin firing induced breakage of stalled forks even in cells with active ATR. Thus, ATR-mediated suppression of dormant origins shields active forks against irreversible breakage via preventing exhaustion of nuclear RPA. This study elucidates how replicating genomes avoid destabilizing DNA damage. Because cancer cells commonly feature intrinsically high replication stress, this study also provides a molecular rationale for their hypersensitivity to ATR inhibitors. Copyright © 2013 Elsevier Inc. All rights reserved.

Habitat-dependent interactions between two size-classes of juvenile steelhead in a small stream

Treesearch

Bret C. Harvey; Rodney J. Nakamoto

1997-01-01

Abstract - The presence of small steelhead (Oncorhynchus mykiss; averaging 55 mm fork length) influenced the growth of larger juvenile steelhead (90 mm fork length) during a 6-week experiment conducted in North Fork Caspar Creek, California, in summer 1994. In fenced replicate deep stream sections in this small stream, growth of the larger steelhead was greater in...

Status of the Mussel Resource in Little South Fork Cumberland River

Treesearch

Melvin L. Warren; Wendell R. Haag; Brooks M. Burr

1999-01-01

As recently as the 198Os, the Little South Fork Cumberland River of southeastern Kentucky supported a diverse freshwater mussel fauna (Starnes and Bogan 1982; Appendix A). The Little South Fork represented one of the last rivers to support a high number of mussel species in the Cumberland River drainage of Kentucky and Tennessee. The river was first surveyed...

A watershed's response to logging and roads: South Fork of Caspar Creek, California, 1967-1976

Treesearch

Raymond M. Rice; Forest B. Tilley; Patricia A. Datzman

1979-01-01

The effect of logging and roadbuilding on erosion and sedimentation are analyzed by comparing the North Fork and South Fork of Caspar Creek, in northern California. Increased sediment production during the 4 years after road construction, was 326 cu yd/sq mi/yr—80 percent greater than that predicted by the predisturbance regression analysis. The average...

16 CFR § 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Requirements for fork and frame assembly. Â... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

Input-form data for the U.S. Geological Survey assessment of the Devonian and Mississippian Bakken and Devonian Three Forks Formations of the U.S. Williston Basin Province, 2013

USGS Publications Warehouse

,; Gaswirth, Stephanie B.; Marra, Kristen R.; Cook, Troy A.; Charpentier, Ronald R.; Gautier, Donald L.; Higley, Debra K.; Klett, Timothy R.; Lewan, Michael D.; Lillis, Paul G.; Schenk, Christopher J.; Tennyson, Marilyn E.; Whidden, Katherine J.

2013-01-01

In 2013, the U.S. Geological Survey assessed the technically recoverable oil and gas resources of the Bakken and Three Forks Formations of the U.S. portion of the Williston Basin. The Bakken and Three Forks Formations were assessed as continuous and hypothetical conventional oil accumulations using a methodology similar to that used in the assessment of other continuous- and conventional-type assessment units throughout the United States. The purpose of this report is to provide supplemental documentation and information used in the Bakken-Three Forks assessment.

Gas Phase Photoacoustic Sensor at 8.41 mu m Using Quartz Tuning Forks and Amplitude Modulated Quantum Cascade Lasers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wojcik, Michael D.; Phillips, Mark C.; Cannon, Bret D.

2006-10-01

We demonstrate the performance of a novel long-wave infrared photoacoustic laser absorbance spectrometer for gas-phase species using an amplitude modulated (AM) quantum cascade (QC) laser and a quartz tuning fork microphone. Photoacoustic signal was generated by focusing the output of a Fabry-Perot QC laser operating at 8.41 ?m between the legs of a quartz tuning fork which served as a transducer for the transient acoustic pressure wave. The QC laser was modulated at the resonant frequency of the tuning fork (32.8 kHz) and delivered a modest 5.3 mW at the tuning fork. This spectrometer was calibrated using the infrared absorbermore » Freon-134a by performing a simultaneous absorption measurement using a 35 cm absorption cell. The NEAS of this instrument was determined to be 2 x 10{sup -8} W cm-1 Hz{sup -1/2}. A corresponding theoretical analysis of the instrument sensitivity is presented and is capable of quantitatively reproducing the experimental NEAS, indicating that the fundamental sensitivity of this technique is limited by the noise floor of the tuning fork itself.« less

Evaluate Status of Pacific Lamprey in the Clearwater River Drainage, Idaho, Annual Report 2002.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cochnauer, Tim; Claire, Christopher

In 2002 Idaho Department of Fish and Game continued investigation into the status of Pacific lamprey populations in Idaho's Clearwater River drainage. Trapping, electrofishing, and spawning ground redd surveys were used to determine Pacific lamprey distribution, life history strategies, and habitat requirements in the South Fork Clearwater River, Lochsa River, Selway River, and Middle Fork Clearwater River subbasins. Five-hundred forty-one ammocoetes were captured electroshocking 70 sites in the South Fork Clearwater River, Lochsa River, Selway River, Middle Fork Clearwater River, Clearwater River, and their tributaries in 2002. Habitat utilization surveys in Red River support previous work indicating Pacific lamprey ammocoetemore » densities are greater in lateral scour pool habitats compared to riffles and rapids. Presence-absence survey findings in 2002 augmented 2000 and 2001 indicating Pacific lamprey macrothalmia and ammocoetes are not numerous or widely distributed. Pacific lamprey distribution was confined to the lower reaches of Red River below rkm 8.0, the South Fork Clearwater River, Lochsa River (Ginger Creek to mouth), Selway River (Race Creek to mouth), Middle Fork Clearwater River, and the Clearwater River (downstream to Potlatch River).« less

Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation

PubMed Central

Georgescu, Roxana; Yuan, Zuanning; Bai, Lin; de Luna Almeida Santos, Ruda; Sun, Jingchuan; Zhang, Dan; Yurieva, Olga; Li, Huilin; O’Donnell, Michael E.

2017-01-01

The eukaryotic CMG (Cdc45, Mcm2–7, GINS) helicase consists of the Mcm2–7 hexameric ring along with five accessory factors. The Mcm2–7 heterohexamer, like other hexameric helicases, is shaped like a ring with two tiers, an N-tier ring composed of the N-terminal domains, and a C-tier of C-terminal domains; the C-tier contains the motor. In principle, either tier could translocate ahead of the other during movement on DNA. We have used cryo-EM single-particle 3D reconstruction to solve the structure of CMG in complex with a DNA fork. The duplex stem penetrates into the central channel of the N-tier and the unwound leading single-strand DNA traverses the channel through the N-tier into the C-tier motor, 5′-3′ through CMG. Therefore, the N-tier ring is pushed ahead by the C-tier ring during CMG translocation, opposite the currently accepted polarity. The polarity of the N-tier ahead of the C-tier places the leading Pol ε below CMG and Pol α-primase at the top of CMG at the replication fork. Surprisingly, the new N-tier to C-tier polarity of translocation reveals an unforeseen quality-control mechanism at the origin. Thus, upon assembly of head-to-head CMGs that encircle double-stranded DNA at the origin, the two CMGs must pass one another to leave the origin and both must remodel onto opposite strands of single-stranded DNA to do so. We propose that head-to-head motors may generate energy that underlies initial melting at the origin. PMID:28096349

Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination

PubMed Central

Fredsøe, Jacob; Nielsen, Ida; Pedersen, Jakob Madsen; Bentsen, Iben Bach; Lisby, Michael; Bjergbaek, Lotte; Andersen, Anni H

2015-01-01

Faithful DNA replication with correct termination is essential for genome stability and transmission of genetic information. Here we have investigated the potential roles of Topoisomerase II (Top2) and the RecQ helicase Sgs1 during late stages of replication. We find that cells lacking Top2 and Sgs1 (or Top3) display two different characteristics during late S/G2 phase, checkpoint activation and accumulation of asymmetric X-structures, which are both independent of homologous recombination. Our data demonstrate that checkpoint activation is caused by a DNA structure formed at the strongest rDNA replication fork barrier (RFB) during replication termination, and consistently, checkpoint activation is dependent on the RFB binding protein, Fob1. In contrast, asymmetric X-structures are formed independent of Fob1 at less strong rDNA replication fork barriers. However, both checkpoint activation and formation of asymmetric X-structures are sensitive to conditions, which facilitate fork merging and progression of replication forks through replication fork barriers. Our data are consistent with a redundant role of Top2 and Sgs1 together with Top3 (Sgs1-Top3) in replication fork merging at rDNA barriers. At RFB either Top2 or Sgs1-Top3 is essential to prevent formation of a checkpoint activating DNA structure during termination, but at less strong rDNA barriers absence of the enzymes merely delays replication fork merging, causing an accumulation of asymmetric termination structures, which are solved over time. PMID:26630413

KSC-02pd0456

NASA Image and Video Library

2002-04-08

KENNEDY SPACE CENTER, FLA. - Space Shuttle Atlantis seems surrounded by birds - most likely pelicans - as it roars into the clear blue sky on mission STS-110. Liftoff occurred at 4:44:19 p.m. EDT (20:41:19 GMT). Carrying the S0 Integrated Truss Structure and Mobile Transporter, STS-110 is the 13th assembly flight to the International Space Station

Giants That Occasionally Roar: Broadcast Regulatory Policy in the United States.

ERIC Educational Resources Information Center

Busby, Linda J.

In this document, broadcast regulatory issues since 1940 are outlined and discussed in relation to social forces. The 1940s saw open warfare between the Federal Communications Commission (FCC) and broadcasters, as a result of the FCC expanding its powers. In 1946, the FCC issued its "Public Service Responsibility of Broadcast Licensees,"…

Intercollegiate Athletics in the Roaring Twenties.

ERIC Educational Resources Information Center

Keefe, Robert J.

College sports were started by students in the post Civil War period of the 1860's and 1870's. By the 1880's football, baseball, crew, and track and field were popular intercollegiate sports. The desire of the nation as a whole for diversion after World War I provided an impetus for sports in general and intercollegiate athletics in particular.…

The "Roar of Chatter" in the Library at San Joaquin Delta College. Research Project.

ERIC Educational Resources Information Center

Evans, Richard B.

Quiet signs and verbal cautioning by library staff do not decrease library noise levels as revealed by two tests using sound measuring equipment at San Joaquin Delta College. The levels in fact increased, confirming previous opinions that signs and staff intervention have little effect on patron behavior. Test methods, data, and five references…

They Work Together to Roar: Kindergartners' Understanding of an Interactive Causal Task

ERIC Educational Resources Information Center

Solis, S. Lynneth; Grotzer, Tina A.

2016-01-01

The aim of this study was to investigate kindergartners' exploration of interactive causality during their play with a pair of toy sound blocks. Interactive causality refers to a type of causal pattern in which two entities interact to produce a causal force, as in particle attraction and symbiotic relationships. Despite being prevalent in nature,…

Sediment transport and storage in North Fork Caspar Creek, Mendocino County, California: water years 1980-1988

Treesearch

Michael Brent Napolitano

1996-01-01

Abstract - The old-growth redwood forest of North Fork Caspar Creek was clear-cut between 1864 and 1904. Previous research on logging-related changes in suspended sediment and streamflow would suggest that North Fork Caspar Creek has recovered from historical logging (Rice et al., 1979; Ziemer, 1981); research on the influence of large woody debris (LWD) on channel...

Environmental Assessment Deicer Recovery at Grand Forks AFB, North Dakota

DTIC Science & Technology

2004-12-15

Air Force Base (AFB), North Dakota. Contacts: 319 CES/CEVA 525 Tuskegee Airmen Boulevard (Blvd) Grand Forks AFB, ND...ACRONYMS, ABBREVIATIONS, AND TERMS AAM Annual Arithmetic Mean ACM Asbestos Containing Material AFB Air Force Base AFI Air Force Instruction AICUZ...meter 10 GFAFB Grand Forks Air Force Base HAP Hazardous Air Pollutants hr Hour H2S Hydrogen Sulfide IRP Installation Restoration

Simulation of effects of ground-water development on water-levels in glacial-drift aquifers in the Brooten-Belgrade area, west-central Minnesota

USGS Publications Warehouse

Delin, G.N.

1991-01-01

The model was used to simulate the steady-state effects of below-normal precipitation (drought) and hypothetical increases in ground-water development. Model results indicate that reduced recharge and increased pumping during a hypothetical 3-year extended drought would lower regional water levels from 2 to 5 feet in each aquifer and as much as 20 feet in the lowermost aquifer zone; ground-water discharge to the East Branch Chippewa and North Fork Crow Rivers would be reduced by 38 percent. The addition of 10 to 20 hypothetical wells in confined aquifers, pumping 123 to 246 million gallons per year, would result in regional water-level declines of 0.1 to 0.5 feet. Simulated water-level declines in wells completed in the lower part of the system would be as much as 5.0 feet as a result of pumping 246 million gallons per year from 20 hypothetical wells. Water-level declines in overlying and underlying aquifers would range from 0.4 to 2.8 feet. Ground-water discharge to the East Branch Chippewa and North Fork Crow Rivers would be unaffected by the pumpage.

Snowmelt runoff modeling in simulation and forecasting modes with the Martinec-Mango model

NASA Technical Reports Server (NTRS)

Shafer, B.; Jones, E. B.; Frick, D. M. (Principal Investigator)

1982-01-01

The Martinec-Rango snowmelt runoff model was applied to two watersheds in the Rio Grande basin, Colorado-the South Fork Rio Grande, a drainage encompassing 216 sq mi without reservoirs or diversions and the Rio Grande above Del Norte, a drainage encompassing 1,320 sq mi without major reservoirs. The model was successfully applied to both watersheds when run in a simulation mode for the period 1973-79. This period included both high and low runoff seasons. Central to the adaptation of the model to run in a forecast mode was the need to develop a technique to forecast the shape of the snow cover depletion curves between satellite data points. Four separate approaches were investigated-simple linear estimation, multiple regression, parabolic exponential, and type curve. Only the parabolic exponential and type curve methods were run on the South Fork and Rio Grande watersheds for the 1980 runoff season using satellite snow cover updates when available. Although reasonable forecasts were obtained in certain situations, neither method seemed ready for truly operational forecasts, possibly due to a large amount of estimated climatic data for one or two primary base stations during the 1980 season.

Continuous water-quality monitoring and regression analysis to estimate constituent concentrations and loads in the Red River of the North at Fargo and Grand Forks, North Dakota, 2003-12

USGS Publications Warehouse

Galloway, Joel M.

2014-01-01

The Red River of the North (hereafter referred to as “Red River”) Basin is an important hydrologic region where water is a valuable resource for the region’s economy. Continuous water-quality monitors have been operated by the U.S. Geological Survey, in cooperation with the North Dakota Department of Health, Minnesota Pollution Control Agency, City of Fargo, City of Moorhead, City of Grand Forks, and City of East Grand Forks at the Red River at Fargo, North Dakota, from 2003 through 2012 and at Grand Forks, N.Dak., from 2007 through 2012. The purpose of the monitoring was to provide a better understanding of the water-quality dynamics of the Red River and provide a way to track changes in water quality. Regression equations were developed that can be used to estimate concentrations and loads for dissolved solids, sulfate, chloride, nitrate plus nitrite, total phosphorus, and suspended sediment using explanatory variables such as streamflow, specific conductance, and turbidity. Specific conductance was determined to be a significant explanatory variable for estimating dissolved solids concentrations at the Red River at Fargo and Grand Forks. The regression equations provided good relations between dissolved solid concentrations and specific conductance for the Red River at Fargo and at Grand Forks, with adjusted coefficients of determination of 0.99 and 0.98, respectively. Specific conductance, log-transformed streamflow, and a seasonal component were statistically significant explanatory variables for estimating sulfate in the Red River at Fargo and Grand Forks. Regression equations provided good relations between sulfate concentrations and the explanatory variables, with adjusted coefficients of determination of 0.94 and 0.89, respectively. For the Red River at Fargo and Grand Forks, specific conductance, streamflow, and a seasonal component were statistically significant explanatory variables for estimating chloride. For the Red River at Grand Forks, a time component also was a statistically significant explanatory variable for estimating chloride. The regression equations for chloride at the Red River at Fargo provided a fair relation between chloride concentrations and the explanatory variables, with an adjusted coefficient of determination of 0.66 and the equation for the Red River at Grand Forks provided a relatively good relation between chloride concentrations and the explanatory variables, with an adjusted coefficient of determination of 0.77. Turbidity and streamflow were statistically significant explanatory variables for estimating nitrate plus nitrite concentrations at the Red River at Fargo and turbidity was the only statistically significant explanatory variable for estimating nitrate plus nitrite concentrations at Grand Forks. The regression equation for the Red River at Fargo provided a relatively poor relation between nitrate plus nitrite concentrations, turbidity, and streamflow, with an adjusted coefficient of determination of 0.46. The regression equation for the Red River at Grand Forks provided a fair relation between nitrate plus nitrite concentrations and turbidity, with an adjusted coefficient of determination of 0.73. Some of the variability that was not explained by the equations might be attributed to different sources contributing nitrates to the stream at different times. Turbidity, streamflow, and a seasonal component were statistically significant explanatory variables for estimating total phosphorus at the Red River at Fargo and Grand Forks. The regression equation for the Red River at Fargo provided a relatively fair relation between total phosphorus concentrations, turbidity, streamflow, and season, with an adjusted coefficient of determination of 0.74. The regression equation for the Red River at Grand Forks provided a good relation between total phosphorus concentrations, turbidity, streamflow, and season, with an adjusted coefficient of determination of 0.87. For the Red River at Fargo, turbidity and streamflow were statistically significant explanatory variables for estimating suspended-sediment concentrations. For the Red River at Grand Forks, turbidity was the only statistically significant explanatory variable for estimating suspended-sediment concentration. The regression equation at the Red River at Fargo provided a good relation between suspended-sediment concentration, turbidity, and streamflow, with an adjusted coefficient of determination of 0.95. The regression equation for the Red River at Grand Forks provided a good relation between suspended-sediment concentration and turbidity, with an adjusted coefficient of determination of 0.96.

Lithostratigraphy of Upper Ordovician strata exposed in Kentucky

USGS Publications Warehouse

Weir, Gordon Whitney; Peterson, Warren Lee; Swadley, W.C.

1984-01-01

Ordovician formations above the Lexington Limestone crop out in the Blue Grass region of Kentucky and along the Cumberland River and its tributaries. The formations are all conformable and in places intertongue and intergrade. The major Ordovician units above the Lexington Limestone in the Blue Grass region are: The Clays Ferry Formation, the Kope Formation, the Garrard Siltstone, the Fairview Formation, the Calloway Creek Limestone, the Grant Lake Limestone, the Ashlock Formation, the Bull Fork Formation, and the Drakes Formation. The Clays Ferry Formation is made up of subequal amounts of fossiliferous limestone and shale and minor siltstone; the Clays Ferry is as much as 300 ft thick and intertongues with the Lexington Limestone and the Kope Formation. The Kope Formation resembles the partly equivalent Clays Ferry but has a higher shale content (60-80 percent) and thicker layers of shale; the Kope, as much as 275 ft thick, is mostly restricted to the northern part of the State. The Garrard Siltstone, which consists of very calcitic siltstone and minor shale, overlies the Clays Ferry Formation in the southeastern part of the Blue Grass region; the Garrard, as much as 100 ft thick, feathers out into the upper part of the Clays Ferry in southern central and northern east-central Kentucky. The Fairview Formation is characterized by even-bedded limestone interlayered with nearly equal amounts of shale and minor siltstone. The Fairview crops out in the northern part of the Blue Grass region, where it generally overlies the Kope Formation or the Garrard Siltstone; it grades southward into the Calloway Creek Limestone. The Calloway Creek contains more limestone (generally at least 70 percent) and is more irregularly and thinner bedded than the Fairview. The Grant Lake Limestone is composed of nodular-bedded limestone (70-90 percent), interlayered and intermixed with shale; it overlies the Fairview Formation in the northern part of the Blue Grass region and the Calloway Creek Limestone in the western and central parts. In east-central Kentucky, the Grant Lake is classified as a member of the Ashlock Formation, an assemblage of lithologically distinct units that were combined to facilitate mapping in the southeastern and southern part of the region. The Ashlock consists of the following members, in ascending order: The Tate (calcitic and dolomitic mudstone), the Grant Lake, the Gilbert (micrograined limestone and shale), the Stingy Creek (nodular-bedded mudstone and limestone), the Terrill (dolomitic and calcitic mudstone), the Sunset (micrograined limestone), and the Reba (nodular-bedded limestone and shale). The Bull Fork Formation, which overlies the Grant Lake Limestone, is made up of subequal amounts of thin-bedded highly fossiliferous limestone and shale; limestone makes up about 80 percent of the basal part of the formation and decreases in abundance irregularly upward to only 20 percent of the top part. On the east side of the Blue Grass region, the Bull Fork grades into the Reba Member of the Ashlock Formation; on the west side, it grades into the Grant Lake. The uppermost formation in the region is the Drakes Formation, which in east-central Kentucky consists of the Rowland Member (calcitic to dolomitic mudstone) overlain by the Preachersville Member (dolomitic to calcitic mudstone and dolomite and dolomitic siltstone). In northeast Kentucky, the Drakes is represented by only the Preachersville Member. In most of central and north-central Kentucky, the formation consists of three members: the Rowland at the base (dolomitic mudstone to muddy limestone), the Bardstown (fossiliferous limestone and shale), and the Saluda Dolomite (dolomite, in part calcitic and muddy). In northern north-central Kentucky, the Drakes is represented by only the Saluda Dolomite Member. The top of the Ordovician sequence in the Blue Grass region is generally formed by members of the Drakes Formation, which are overlain by str

Rinne test: does the tuning fork position affect the sound amplitude at the ear?

PubMed

Butskiy, Oleksandr; Ng, Denny; Hodgson, Murray; Nunez, Desmond A

2016-03-24

Guidelines and text-book descriptions of the Rinne test advise orienting the tuning fork tines in parallel with the longitudinal axis of the external auditory canal (EAC), presumably to maximise the amplitude of the air conducted sound signal at the ear. Whether the orientation of the tuning fork tines affects the amplitude of the sound signal at the ear in clinical practice has not been previously reported. The present study had two goals: determine if (1) there is clinician variability in tuning fork placement when presenting the air-conduction stimulus during the Rinne test; (2) the orientation of the tuning fork tines, parallel versus perpendicular to the EAC, affects the sound amplitude at the ear. To assess the variability in performing the Rinne test, the Canadian Society of Otolaryngology - Head and Neck Surgery members were surveyed. The amplitudes of the sound delivered to the tympanic membrane with the activated tuning fork tines held in parallel, and perpendicular to, the longitudinal axis of the EAC were measured using a Knowles Electronics Mannequin for Acoustic Research (KEMAR) with the microphone of a sound level meter inserted in the pinna insert. 47.4 and 44.8% of 116 survey responders reported placing the fork parallel and perpendicular to the EAC respectively. The sound intensity (sound-pressure level) recorded at the tympanic membrane with the 512 Hz tuning fork tines in parallel with as opposed to perpendicular to the EAC was louder by 2.5 dB (95% CI: 1.35, 3.65 dB; p < 0.0001) for the fundamental frequency (512 Hz), and by 4.94 dB (95% CI: 3.10, 6.78 dB; p < 0.0001) and 3.70 dB (95% CI: 1.62, 5.78 dB; p = .001) for the two harmonic (non-fundamental) frequencies (1 and 3.15 kHz), respectively. The 256 Hz tuning fork in parallel with the EAC as opposed to perpendicular to was louder by 0.83 dB (95% CI: -0.26, 1.93 dB; p = 0.14) for the fundamental frequency (256 Hz), and by 4.28 dB (95% CI: 2.65, 5.90 dB; p < 0.001) and 1.93 dB (95% CI: 0.26, 3.61 dB; p = .02) for the two harmonic frequencies (500 and 4 kHz) respectively. Clinicians vary in their orientation of the tuning fork tines in relation to the EAC when performing the Rinne test. Placement of the tuning fork tines in parallel as opposed to perpendicular to the EAC results in a higher sound amplitude at the level of the tympanic membrane.

Geochemical map of the North Fork John Day River Roadless Area, Grant County, Oregon

USGS Publications Warehouse

Evans, James G.

1986-01-01

The North Fork John Day River Roadless Area comprised 21,210 acres in the Umatilla and Wallowa-Whitman National Forests, Grant County, Oregon, about 30 miles northwest of Baker, Oregon. The irregularly shaped area extends for about 1 mile on both sides of a 25-mile segment of the North Fork John Day River from Big Creek on the west to North Fork John Day Campground on the east. Most of the roadless area is in the northern half of the Desolation Butte 15-minute quadrangle. The eastern end of the area is in parts of the Granite and Trout Meadows 7½-minute quadrangles.

Cancer therapy and replication stress: forks on the road to perdition.

PubMed

Kotsantis, Panagiotis; Jones, Rebecca M; Higgs, Martin R; Petermann, Eva

2015-01-01

Deregulated DNA replication occurs in cancer where it contributes to genomic instability. This process is a target of cytotoxic therapies. Chemotherapies exploit high DNA replication in cancer cells by modifying the DNA template or by inhibiting vital enzymatic activities that lead to slowing or stalling replication fork progression. Stalled replication forks can be converted into toxic DNA double-strand breaks resulting in cell death, i.e., replication stress. While likely crucial for many cancer treatments, replication stress is poorly understood due to its complexity. While we still know relatively little about the role of replication stress in cancer therapy, technical advances in recent years have shed new light on the effect that cancer therapeutics have on replication forks and the molecular mechanisms that lead from obstructed fork progression to cell death. This chapter will give an overview of our current understanding of replication stress in the context of cancer therapy. © 2015 Elsevier Inc. All rights reserved.

Assembly of Slx4 signaling complexes behind DNA replication forks.

PubMed

Balint, Attila; Kim, TaeHyung; Gallo, David; Cussiol, Jose Renato; Bastos de Oliveira, Francisco M; Yimit, Askar; Ou, Jiongwen; Nakato, Ryuichiro; Gurevich, Alexey; Shirahige, Katsuhiko; Smolka, Marcus B; Zhang, Zhaolei; Brown, Grant W

2015-08-13

Obstructions to replication fork progression, referred to collectively as DNA replication stress, challenge genome stability. In Saccharomyces cerevisiae, cells lacking RTT107 or SLX4 show genome instability and sensitivity to DNA replication stress and are defective in the completion of DNA replication during recovery from replication stress. We demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. Slx4 complex formation is nucleated by Mec1 phosphorylation of histone H2A, which is recognized by the constitutive Slx4 binding partner Rtt107. Slx4 is essential for recruiting the Mec1 activator Dpb11 behind stressed replication forks, and Slx4 complexes are important for full activity of Mec1. We propose that Slx4 complexes promote robust checkpoint signaling by Mec1 by stably recruiting Dpb11 within a discrete domain behind the replication fork, during DNA replication stress. © 2015 The Authors.

Using Caffeine as a Water Quality Indicator in the Ambient Monitoring Program for Third Fork Creek Watershed, Durham, North Carolina

PubMed Central

Spence, Porché L

2015-01-01

Caffeine has been suggested as a chemical indicator for domestic wastewater in freshwater systems, although it is not included in water quality monitoring programs. The Third Fork Creek watershed in Durham, NC, is highly urbanized, with a history of receiving untreated wastewater from leaking and overflowing sanitary sewers. The poor water quality originating in the Third Fork Creek watershed threatens its intended uses and jeopardizes drinking water, aquatic life, and recreational activities provided by Jordan Lake. Organic waste contaminants have been detected in both Third Fork Creek watershed and Jordan Lake; however, the sampling periods were temporary, resulting in a few samples collected during nonstorm periods. It is recommended that (1) the concentration of caffeine and other organic waste contaminants are determined during storm and nonstorm periods and (2) caffeine is monitored regularly with traditional water quality indicators to evaluate the health of Third Fork Creek watershed. PMID:26157335

Ecological condition of the East Fork of the Gila River and selected tributaries: Gila National Forest, New Mexico

Treesearch

Robert D. Ohmart

1996-01-01

Ecological condition of riparian habitats along the East Fork of the Gila River, Main Diamond Creek, lower South Diamond Creek, and Black Canyon Creek are all in very heavily degraded condition. Channel cross-sections show extensive entrenchment, high width-to-depth ratios, and numerous reaches where banks are sloughing into the stream, especially on the East Fork of...

The Sound Field around a Tuning Fork and the Role of a Resonance Box

ERIC Educational Resources Information Center

Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

2015-01-01

Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: (1) Why is a bare tuning fork such a weak emitter of sound? (2) What is the role of the…

Persistence of historical logging impacts on channel form in mainstem North Fork Caspar Creek

Treesearch

Michael B. Napolitano

1998-01-01

The old-growth redwood forest of North Fork Caspar Creek was clear-cut logged between 1860 and 1904. Transportation of logs involved construction of a splash dam in the headwaters of North Fork Caspar Creek. Water stored behind the dam was released during large storms to enable log drives. Before log drives could be conducted, the stream channel had to be prepared by...

Qualitative Assessment: Evaluating the Impacts of Climate ...

EPA Pesticide Factsheets

The South Fork Nooksack River (South Fork) is located in northwest Washington State and is home to nine species of Pacific salmon, including Nooksack early Chinook (aka, spring Chinook salmon), an iconic species for the Nooksack Indian Tribe. The quantity of salmon in the South Fork, especially spring Chinook salmon, has dramatically declined from historic levels, due primarily to habitat degradation from the legacy impacts of various land uses such as commercial forestry, agriculture, flood control, and transportation infrastructure. Segments of the South Fork and some of its tributaries exceed temperature criteria established for the protection of cold-water salmonid populations, and were listed on Washington State’s Clean Water Act (CWA) 303(d) list of impaired waterbodies. High water temperatures in the South Fork are detrimental to fish and other native species that depend on cool, clean, well-oxygenated water. Of the nine salmon species, three have been listed as threatened under the federal Endangered Species Act (ESA) and are of high priority to restoration efforts in the South Fork—spring Chinook salmon, summer steelhead trout, and bull trout. Growing evidence shows that climate change will exacerbate legacy impacts. This qualitative assessment is a comprehensive analysis of climate change impacts on freshwater habitat and Pacific salmon in the South Fork. It also evaluates the effectiveness of restoration tools that address Pacific salmon recovery.

The PriA Replication Restart Protein Blocks Replicase Access Prior to Helicase Assembly and Directs Template Specificity through Its ATPase Activity*

PubMed Central

Manhart, Carol M.; McHenry, Charles S.

2013-01-01

The PriA protein serves as an initiator for the restart of DNA replication on stalled replication forks and as a checkpoint protein that prevents the replicase from advancing in a strand displacement reaction on forks that do not contain a functional replicative helicase. We have developed a primosomal protein-dependent fluorescence resonance energy transfer (FRET) assay using a minimal fork substrate composed of synthetic oligonucleotides. We demonstrate that a self-loading reaction, which proceeds at high helicase concentrations, occurs by threading of a preassembled helicase over free 5′-ends, an event that can be blocked by attaching a steric block to the 5′-end or coating DNA with single-stranded DNA binding protein. The specificity of PriA for replication forks is regulated by its intrinsic ATPase. ATPase-defective PriA K230R shows a strong preference for substrates that contain no gap between the leading strand and the duplex portion of the fork, as demonstrated previously. Wild-type PriA prefers substrates with larger gaps, showing maximal activity on substrates on which PriA K230R is inactive. We demonstrate that PriA blocks replicase function on forks by blocking its binding. PMID:23264623

Quartz tuning-fork oscillations in He II and drag coefficient

NASA Astrophysics Data System (ADS)

Gritsenko, I. A.; Zadorozhko, A. A.; Neoneta, A. S.; Chagovets, V. K.; Sheshin, G. A.

2011-07-01

The temperature dependencies of drag coefficient for quartz tuning forks of various geometric dimensions, immersed in the He II, were determined experimentally in the temperature range 0.1-3 K. It is identified, that these dependencies are similar, but the values of drag coefficient are different for tuning forks with different geometric dimensions. It is shown, that the obtained specific drag coefficient depends only on the temperature and frequency of vibrations, when the value of drag coefficient is normalized to the surface area of moving tuning-fork prong. The temperature dependencies of normalized drag coefficient for the tuning forks of various dimensions, wire, and microsphere, oscillating in the Не II, are compared. It is shown, that in the ballistic regime of scattering of quasiparticles, these dependencies are identical and have a slope proportional to T4, which is determined by the density of thermal excitations. In the hydrodynamic regime at T > 0.5 K, the behavior of the temperature dependence of specific drag coefficient is affected by the size and frequency of vibrating body. The empirical relation, which allows to describe the behavior of specific drag coefficient for vibrating tuning forks, microsphere, and wire everywhere over the temperature region and at various frequencies, is proposed.

Movements of adult chinook salmon during spawning migration in a metals-contaminated system, Coeur d'Alene River, Idaho

USGS Publications Warehouse

Goldstein, J.N.; Woodward, D.F.; Farag, A.M.

1999-01-01

Spawning migration of adult male chinook salmon Oncorhynchus tshawytscha was monitored by radio telemetry to determine their response to the presence of metals contamination in the South Fork of the Coeur d'Alene River, Idaho. The North Fork of the Coeur d'Alene River is relatively free of metals contamination and was used as a control. In all, 45 chinook salmon were transported from their natal stream, Wolf Lodge Creek, tagged with radio transmitters, and released in the Coeur d'Alene River 2 km downstream of the confluence of the South Fork and the North Fork of the Coeur d'Alene River. Fixed telemetry receivers were used to monitor the upstream movement of the tagged chinook salmon through the confluence area for 3 weeks after release. During this period, general water quality and metals concentrations were monitored in the study area. Of the 23 chinook salmon observed to move upstream from the release site and through the confluence area, the majority (16 fish, 70%) moved up the North Fork, and only 7 fish (30%) moved up the South Fork, where greater metals concentrations were observed. Our results agree with laboratory findings and suggest that natural fish populations will avoid tributaries with high metals contamination.

Simultaneous binding to the tracking strand, displaced strand and the duplex of a DNA fork enhances unwinding by Dda helicase

PubMed Central

Aarattuthodiyil, Suja; Byrd, Alicia K.; Raney, Kevin D.

2014-01-01

Interactions between helicases and the tracking strand of a DNA substrate are well-characterized; however, the role of the displaced strand is a less understood characteristic of DNA unwinding. Dda helicase exhibited greater processivity when unwinding a DNA fork compared to a ss/ds DNA junction substrate. The lag phase in the unwinding progress curve was reduced for the forked DNA compared to the ss/ds junction. Fewer kinetic steps were required to unwind the fork compared to the ss/ds junction, suggesting that binding to the fork leads to disruption of the duplex. DNA footprinting confirmed that interaction of Dda with a fork leads to two base pairs being disrupted whereas no disruption of base pairing was observed with the ss/ds junction. Neutralization of the phosphodiester backbone resulted in a DNA-footprinting pattern similar to that observed with the ss/ds junction, consistent with disruption of the interaction between Dda and the displaced strand. Several basic residues in the 1A domain which were previously proposed to bind to the incoming duplex DNA were replaced with alanines, resulting in apparent loss of interaction with the duplex. Taken together, these results suggest that Dda interaction with the tracking strand, displaced strand and duplex coordinates DNA unwinding. PMID:25249618

Design and Theoretical Analysis of a Resonant Sensor for Liquid Density Measurement

PubMed Central

Zheng, Dezhi; Shi, Jiying; Fan, Shangchun

2012-01-01

In order to increase the accuracy of on-line liquid density measurements, a sensor equipped with a tuning fork as the resonant sensitive component is designed in this paper. It is a quasi-digital sensor with simple structure and high precision. The sensor is based on resonance theory and composed of a sensitive unit and a closed-loop control unit, where the sensitive unit consists of the actuator, the resonant tuning fork and the detector and the closed-loop control unit comprises precondition circuit, digital signal processing and control unit, analog-to-digital converter and digital-to-analog converter. An approximate parameters model of the tuning fork is established and the impact of liquid density, position of the tuning fork, temperature and structural parameters on the natural frequency of the tuning fork are also analyzed. On this basis, a tuning fork liquid density measurement sensor is developed. In addition, experimental testing on the sensor has been carried out on standard calibration facilities under constant 20 °C, and the sensor coefficients are calibrated. The experimental results show that the repeatability error is about 0.03% and the accuracy is about 0.4 kg/m3. The results also confirm that the method to increase the accuracy of liquid density measurement is feasible. PMID:22969378

Design and theoretical analysis of a resonant sensor for liquid density measurement.

PubMed

Zheng, Dezhi; Shi, Jiying; Fan, Shangchun

2012-01-01

In order to increase the accuracy of on-line liquid density measurements, a sensor equipped with a tuning fork as the resonant sensitive component is designed in this paper. It is a quasi-digital sensor with simple structure and high precision. The sensor is based on resonance theory and composed of a sensitive unit and a closed-loop control unit, where the sensitive unit consists of the actuator, the resonant tuning fork and the detector and the closed-loop control unit comprises precondition circuit, digital signal processing and control unit, analog-to-digital converter and digital-to-analog converter. An approximate parameters model of the tuning fork is established and the impact of liquid density, position of the tuning fork, temperature and structural parameters on the natural frequency of the tuning fork are also analyzed. On this basis, a tuning fork liquid density measurement sensor is developed. In addition, experimental testing on the sensor has been carried out on standard calibration facilities under constant 20 °C, and the sensor coefficients are calibrated. The experimental results show that the repeatability error is about 0.03% and the accuracy is about 0.4 kg/m(3). The results also confirm that the method to increase the accuracy of liquid density measurement is feasible.

KSC-02pp0465

NASA Image and Video Library

2002-04-08

KENNEDY SPACE CENTER, FLA. -- Launch! Birds in the foreground seem oblivious to the fire and smoke as Space Shuttle Atlantis roars into the sky on mission STS-110. Liftoff occurred at 4:44:19 p.m. EDT (20:41:19 GMT). Carrying the S0 Integrated Truss Structure and Mobile Transporter, STS-110 is the 13th assembly flight to the International Space Station

Meteorological factors in the Quartz Creek forest fire

Treesearch

H. T. Gisborne

1927-01-01

It is not often that a large forest fire occurs conveniently near a weather station specially equipped for measuring forest-fire weather. The 13,000-acre Quartz Creek fire on the Kaniksu National Forest during the summer of 1936 was close enough to the Priest River Experimental Forest of the Northern Rocky Mountain Forest Experiment Station for the roar of the flumes...

"The Mouse that Dared to Roar": Youths and the Camden Controversy

ERIC Educational Resources Information Center

Al-Natour, Ryan

2010-01-01

In late 2007, a proposal for an Islamic school in Camden, a semi-rural area located in the greater Sydney region, sparked concern among local residents. This paper examines the discourses relating to representations of young people in Camden at that time, as expressed by both young people and adults. It explores the prominent literature on moral…

Sound. Physical Science in Action[TM]. Schlessinger Science Library. [Videotape].

ERIC Educational Resources Information Center

2000

A door closes. A horn beeps. A crowd roars. Sound waves travel outward in all directions from the source. They can all be heard, but how? Did they travel directly to the ears? Perhaps they bounced off another object first or traveled through a different medium, changing speed along the way. Students learn how sound waves travel and about their…

Development and Functions of Signature Whistles of Free-Ranging Bottlenose Dolphins, Tursiops truncatus

DTIC Science & Technology

1992-09-01

34 (Waser 1977), "loud calls" of lion tailed macaques (Green 1981), long calls of orangutans (Galdikas and Insley 1988), and gorilla "roars" (Marler and...whistles and echolocation clicks. Galdikas, B. M. F. and S. J. Insley. 1988. The fast call of the adult male orangutan . J. Mamm. 69: 371-375. Gould, E

Early Rockets

NASA Image and Video Library

2004-04-15

According to one ancient legend, a Chinese official named Wan Hoo attempted a flight to the moon using a large wicker chair to which were fastened 47 large rockets. Forty seven assistants, each armed with a torch, rushed forward to light the fuses. In a moment, there was a tremendous roar accompanied by billowing clouds of smoke. When the smoke cleared, the flying chair and Wan Hoo were gone.

Hopewell Furnace: A Pennsylvania Iron-Making Plantation. Teaching with Historic Places.

ERIC Educational Resources Information Center

Koman, Rita G.

The rhythmic noises of the turning water wheel and the roar of the furnace blast never stopped at Hopewell Furnace (Pennsylvania) during its years of operation (1771-1883). As long as the furnace was in blast, the ironworkers' jobs were safe. In case of trouble, they could escape to the woods, fields, and creeks of rural Pennsylvania. Now a…

Rapid overt airborne reconnaissance (ROAR) for mines and obstacles in very shallow water, surf zone, and beach

NASA Astrophysics Data System (ADS)

Moran, Steven E.; Austin, William L.; Murray, James T.; Roddier, Nicolas A.; Bridges, Robert; Vercillo, Richard; Stettner, Roger; Phillips, Dave; Bisbee, Al; Witherspoon, Ned H.

2003-09-01

Under the Office of Naval Research's Organic Mine Countermeasures Future Naval Capabilities (OMCM FNC) program, Lite Cycles, Inc. is developing an innovative and highly compact airborne active sensor system for mine and obstacle detection in very shallow water (VSW), through the surf-zone (SZ) and onto the beach. The system uses an innovative LCI proprietary integrated scanner, detector, and telescope (ISDT) receiver architecture. The ISD tightly couples all receiver components and LIDAR electronics to achieve the system compaction required for tactical UAVintegration while providing a large aperture. It also includes an advanced compact multifunction laser transmitter; an industry-first high-resolution, compact 3-D camera, a scanning function for wide area search, and temporally displaced multiple looks on the fly over the ocean surface for clutter reduction. Additionally, the laser will provide time-multiplexed multi-color output to perform day/night multispectral imaging for beach surveillance. New processing algorithms for mine detection in the very challenging surf-zone clutter environment are under development, which offer the potential for significant processing gains in comparison to the legacy approaches. This paper reviews the legacy system approaches, describes the mission challenges, and provides an overview of the ROAR system architecture.

Holocene alluvial stratigraphy and response to climate change in the Roaring River valley, Front Range, Colorado, USA

USGS Publications Warehouse

Madole, Richard F.

2012-01-01

Stratigraphic analyses and radiocarbon geochronology of alluvial deposits exposed along the Roaring River, Colorado, lead to three principal conclusions: (1) the opinion that stream channels in the higher parts of the Front Range are relics of the Pleistocene and nonalluvial under the present climate, as argued in a water-rights trial USA v. Colorado, is untenable, (2) beds of clast-supported gravel alternate in vertical succession with beds of fine-grained sediment (sand, mud, and peat) in response to centennial-scale changes in snowmelt-driven peak discharges, and (3) alluvial strata provide information about Holocene climate history that complements the history provided by cirque moraines, periglacial deposits, and paleontological data. Most alluvial strata are of late Holocene age and record, among other things, that: (1) the largest peak flows since the end of the Pleistocene occurred during the late Holocene; (2) the occurrence of a mid- to late Holocene interval (~2450–1630(?) cal yr BP) of warmer climate, which is not clearly identified in palynological records; and (3) the Little Ice Age climate seems to have had little impact on stream channels, except perhaps for minor (~1 m) incision. Published

Holocene alluvial stratigraphy and response to climate change in the Roaring River valley, Front Range, Colorado, USA

NASA Astrophysics Data System (ADS)

Madole, Richard F.

2012-09-01

Stratigraphic analyses and radiocarbon geochronology of alluvial deposits exposed along the Roaring River, Colorado, lead to three principal conclusions: (1) the opinion that stream channels in the higher parts of the Front Range are relics of the Pleistocene and nonalluvial under the present climate, as argued in a water-rights trial USA v. Colorado, is untenable, (2) beds of clast-supported gravel alternate in vertical succession with beds of fine-grained sediment (sand, mud, and peat) in response to centennial-scale changes in snowmelt-driven peak discharges, and (3) alluvial strata provide information about Holocene climate history that complements the history provided by cirque moraines, periglacial deposits, and paleontological data. Most alluvial strata are of late Holocene age and record, among other things, that: (1) the largest peak flows since the end of the Pleistocene occurred during the late Holocene; (2) the occurrence of a mid- to late Holocene interval (~ 2450-1630(?) cal yr BP) of warmer climate, which is not clearly identified in palynological records; and (3) the Little Ice Age climate seems to have had little impact on stream channels, except perhaps for minor (~ 1 m) incision.

Archaeological Investigations in the Halls-Fowlkes Region South Fork of the Forked Deer River, West Tennessee

DTIC Science & Technology

1985-01-01

Obion-Forked Deer River and Reelfoot -Indian Creek drainages (Smith 1979a), the Mud Creek drainage (Dye 1975), the Cypress Creek drainages (Peterson 1975...sites have been identified by the presence of Palmer, Cypress Creek, Lost Lake , Decatur, Kirk Stemmed, Big Sandy, Plevna, Haywood, Kirk Corner Notched...necessary to clarify this problem. Several different Mississippian phases, including the Walls, Boxtown, Ensley, Tiptonville and Reelfoot phases have

UNIDENTIFIED CATENARY SUSPENSION BRIDGE ON RIVETED METAL PIERS, SHOWING HOWE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

UNIDENTIFIED CATENARY SUSPENSION BRIDGE ON RIVETED METAL PIERS, SHOWING HOWE PIPE TRUSS RAILING AND TRUSSED DECK BEAMS TYPICAL TO BRIDGES BUILT BY FLINN-MOYER COMPANY. TRIPODAL PIPE TOWERS RESEMBLE CLEAR FORK OF THE BRAZOS SUSPENSION BRIDGE’S TOWERS PRIOR TO ENCASEMENT IN CONCRETE. NOTE COLLAPSED TRUSS IN RIVER. ELEVATION VIEW. - Clear Fork of Brazos River Suspension Bridge, Spanning Clear Fork of Brazos River at County Route 179, Albany, Shackelford County, TX

Study on vacuum packaging reliability of micromachined quartz tuning fork gyroscopes

NASA Astrophysics Data System (ADS)

Fan, Maoyan; Zhang, Lifang

2017-09-01

Packaging technology of the micromachined quartz tuning fork gyroscopes by vacuum welding has been experimentally studied. The performance of quartz tuning fork is influenced by the encapsulation shell, encapsulation method and fixation of forks. Alloy solder thick film is widely used in the package to avoid the damage of the chip structure by the heat resistance and hot temperature, and this can improve the device performance and welding reliability. The results show that the bases and the lids plated with gold and nickel can significantly improve the airtightness and reliability of the vacuum package. Vacuum packaging is an effective method to reduce the vibration damping, improve the quality factor and further enhance the performance. The threshold can be improved nearly by 10 times.

Maps showing mines, quarries, prospects, and exposures in the Devils Fork Roadless Area, Scott County, Virginia

USGS Publications Warehouse

Behum, Paul T.

1984-01-01

The Devils Fork Roadless Area is located at the eastern edge of the Appalachian coal region and is within the Cumberland Mountain section of the Appalachian Plateau physiographic province. Most of the area is drained by Devil Fork and its tributaries. Clinch Rock Branch of Straight Creek, Roddy Branch of Valley Creek, and Stinking Creek, all tributary to the Clinch River, drain small fringe tracts. Altitudes range from about 1,550 ft on the lower part of Straight Fork to about 3,490 ft at Cox Place on Little Mountain. Vegetation varies from mixed hardwoods in the uplands to thickets of conifer, rhododendron, and laurel in moist protected areas, as in coves along drainage courses.

Replication Fork Protection Factors Controlling R-Loop Bypass and Suppression.

PubMed

Chang, Emily Yun-Chia; Stirling, Peter C

2017-01-14

Replication-transcription conflicts have been a well-studied source of genome instability for many years and have frequently been linked to defects in RNA processing. However, recent characterization of replication fork-associated proteins has revealed that defects in fork protection can directly or indirectly stabilize R-loop structures in the genome and promote transcription-replication conflicts that lead to genome instability. Defects in essential DNA replication-associated activities like topoisomerase, or the minichromosome maintenance (MCM) helicase complex, as well as fork-associated protection factors like the Fanconi anemia pathway, both appear to mitigate transcription-replication conflicts. Here, we will highlight recent advances that support the concept that normal and robust replisome function itself is a key component of mitigating R-loop coupled genome instability.

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation...

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation...

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation...

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation...

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation Beaver...

Assessment of possible sources of microbiological contamination in the water column and streambed sediment of the Jacks Fork, Ozark National Scenic Riverways, Missouri - Phase III

USGS Publications Warehouse

Davis, Jerri V.; Barr, Miya N.

2006-01-01

In 1998, a 5 river-mile reach of the Jacks Fork was included on Missouri's list of impaired waters as required by Section 303(d) of the Federal Clean Water Act. The identified pollutant on the Jacks Fork was fecal coliform bacteria. The length of the impaired reach was changed to 7 miles on the Missouri 2002 303(d) list because of data indicating the fecal coliform bacteria problem existed over a broader area. The U.S. Geological Survey, in cooperation with the National Park Service, conducted a study to better understand the extent and sources of microbiological contamination within the Jacks Fork from Alley Spring to the mouth, which includes the 7-mile 303(d) reach. Ten sites were sampled from June 2003 through October 2003 and from June 2004 through October 2004. Water-column and streambed sediment samples were collected from main-stem and tributary sites mostly during base-flow conditions during a variety of recreational season river uses and analyzed for fecal coliform and Escherichia coli bacteria. Isolates of Escherichia coli obtained from water samples collected at five sites were submitted for rep-PCR analysis to identify presumptive sources of fecal indicator bacteria in the Jacks Fork. Results indicate that recreational users (including boaters and swimmers) are not the primary source of fecal coliform bacteria in the Jacks Fork; rather, the presence of fecal coliform bacteria is associated with other animals, of which horses are the primary source. Increases in fecal coliform bacteria densities in the Jacks Fork are associated with cross-country horseback trail-riding events.

Water-Quality, Bed-Sediment, and Biological Data (October 2005 through September 2006) and Statistical Summaries of Long-Term Data for Streams in the Clark Fork Basin, Montana

USGS Publications Warehouse

Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

2007-01-01

Water, bed sediment, and biota were sampled in streams from Butte to below Milltown Reservoir as part of a long-term monitoring program in the upper Clark Fork basin; additional water-quality samples were collected in the Clark Fork basin from sites near Milltown Reservoir downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water-quality samples were collected periodically at 22 sites from October 2005 through September 2006. Bed-sediment and biological samples were collected once at 12 sites during August 2006. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2005 through September 2006. Water-quality data include concentrations of selected major ions, trace ele-ments, and suspended sediment. Nutrients also were analyzed in the supplemental water-quality samples. Daily values of suspended-sed-iment concentration and suspended-sediment discharge were determined for four sites, and seasonal daily values of turbidity were determined for four sites. Bed-sediment data include trace-ele-ment concentrations in the fine-grained fraction. Bio-logical data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

Stream-Sediment Geochemistry in Mining-Impacted Drainages of the Yankee Fork of the Salmon River, Custer County, Idaho

USGS Publications Warehouse

Frost, Thomas P.; Box, Stephen E.

2009-01-01

This reconnaissance study was undertaken at the request of the USDA Forest Service, Region 4, to assess the geochemistry, in particular the mercury and selenium contents, of mining-impacted sediments in the Yankee Fork of the Salmon River in Custer County Idaho. The Yankee Fork has been the site of hard-rock and placer mining, primarily for gold and silver, starting in the 1880s. Major dredge placer mining from the 1930s to 1950s in the Yankee Fork disturbed about a 10-kilometer reach. Mercury was commonly used in early hard-rock mining and placer operations for amalgamation and recovery of gold. During the late 1970s, feasibility studies were done on cyanide-heap leach recovery of gold from low-grade ores of the Sunbeam and related deposits. In the mid-1990s a major open-pit bulk-vat leach operation was started at the Grouse Creek Mine. This operation shut down when gold values proved to be lower than expected. Mercury in stream sediments in the Yankee Fork ranges from below 0.02 ppm to 7 ppm, with the highest values associated with old mill locations and lode and placer mines. Selenium ranges from below the detection limit for this study of 0.2 ppm to 4 ppm in Yankee Fork sediment samples. The generally elevated selenium content in the sediment samples reflect the generally high selenium contents in the volcanic rocks that underlie the Yankee Fork and the presence of gold and silver selenides in some of the veins that were exploited in the early phases of mining.

Surface-water quality assessment of the North Fork Red River basin upstream from Lake Altus, Oklahoma, 2002

USGS Publications Warehouse

Smith, S. Jerrod; Schneider, M.L.; Masoner, J.R.; Blazs, R.L.

2003-01-01

Elevated salinity in the North Fork Red River is a major concern of the Bureau of Reclamation W. C. Austin Project at Lake Altus. Understanding the relation between surface-water runoff, ground-water discharge, and surface-water quality is important for maintaining the beneficial use of water in the North Fork Red River basin. Agricultural practices, petroleum production, and natural dissolution of salt-bearing bedrock have the potential to influence the quality of nearby surface water. The U.S. Geological Survey, in cooperation with the Bureau of Reclamation, sampled stream discharge and water chemistry at 19 stations on the North Fork Red River and tributaries. To characterize surface-water resources of the basin in a systematic manner, samples were collected synoptically during receding streamflow conditions during July 8-11, 2002. Together, sulfate and chloride usually constitute greater than half of the dissolved solids. Concentrations of sulfate ranged from 87.1 to 3,450 milligrams per liter. The minimum value was measured at McClellan Creek near Back (07301220), and the maximum value was measured at Bronco Creek near Twitty (07301303). Concentrations of chloride ranged from 33.2 to 786 milligrams per liter. The minimum value was measured at a North Fork Red River tributary (unnamed) near Twitty (07301310), and the maximum value was measured at the North Fork Red River near Back (07301190), the most upstream sample station.

Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.

PubMed

Parajuli, Shankar; Teasley, Daniel C; Murali, Bhavna; Jackson, Jessica; Vindigni, Alessandro; Stewart, Sheila A

2017-09-15

Faithful DNA replication is essential for genome stability. To ensure accurate replication, numerous complex and redundant replication and repair mechanisms function in tandem with the core replication proteins to ensure DNA replication continues even when replication challenges are present that could impede progression of the replication fork. A unique topological challenge to the replication machinery is posed by RNA-DNA hybrids, commonly referred to as R-loops. Although R-loops play important roles in gene expression and recombination at immunoglobulin sites, their persistence is thought to interfere with DNA replication by slowing or impeding replication fork progression. Therefore, it is of interest to identify DNA-associated enzymes that help resolve replication-impeding R-loops. Here, using DNA fiber analysis, we demonstrate that human ribonuclease H1 (RNH1) plays an important role in replication fork movement in the mammalian nucleus by resolving R-loops. We found that RNH1 depletion results in accumulation of RNA-DNA hybrids, slowing of replication forks, and increased DNA damage. Our data uncovered a role for RNH1 in global DNA replication in the mammalian nucleus. Because accumulation of RNA-DNA hybrids is linked to various human cancers and neurodegenerative disorders, our study raises the possibility that replication fork progression might be impeded, adding to increased genomic instability and contributing to disease. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

BRCA2 and RAD51 promote double-strand break formation and cell death in response to gemcitabine.

PubMed

Jones, Rebecca M; Kotsantis, Panagiotis; Stewart, Grant S; Groth, Petra; Petermann, Eva

2014-10-01

Replication inhibitors cause replication fork stalling and double-strand breaks (DSB) that result from processing of stalled forks. During recovery from replication blocks, the homologous recombination (HR) factor RAD51 mediates fork restart and DSB repair. HR defects therefore sensitize cells to replication inhibitors, with clear implications for cancer therapy. Gemcitabine is a potent replication inhibitor used to treat cancers with mutations in HR genes such as BRCA2. Here, we investigate why, paradoxically, mutations in HR genes protect cells from killing by gemcitabine. Using DNA replication and DNA damage assays in mammalian cells, we show that even short gemcitabine treatments cause persistent replication inhibition. BRCA2 and RAD51 are recruited to chromatin early after removal of the drug, actively inhibit replication fork progression, and promote the formation of MUS81- and XPF-dependent DSBs that remain unrepaired. Our data suggest that HR intermediates formed at gemcitabine-stalled forks are converted into DSBs and thus contribute to gemcitabine-induced cell death, which could have implications for the treatment response of HR-deficient tumors. ©2014 American Association for Cancer Research.

BRCA2 and RAD51 promote double-strand break formation and cell death in response to Gemcitabine

PubMed Central

Jones, Rebecca M.; Kotsantis, Panagiotis; Stewart, Grant S.; Groth, Petra; Petermann, Eva

2014-01-01

Replication inhibitors cause replication fork stalling and double-strand breaks (DSBs) that result from processing of stalled forks. During recovery from replication blocks, the homologous recombination (HR) factor RAD51 mediates fork restart and DSB repair. HR defects therefore sensitise cells to replication inhibitors, with clear implications for cancer therapy. Gemcitabine is a potent replication inhibitor used to treat cancers with mutations in HR genes such as BRCA2. Here we investigate why, paradoxically, mutations in HR genes protect cells from killing by Gemcitabine. Using DNA replication and -damage assays in mammalian cells, we show that even short Gemcitabine treatments cause persistent replication inhibition. BRCA2 and RAD51 are recruited to chromatin early after removal of the drug, actively inhibit replication fork progression and promote the formation of MUS81- and XPF-dependent DSBs that remain unrepaired. Our data suggest that HR intermediates formed at Gemcitabine-stalled forks are converted into DSBs and thus contribute to Gemcitabine-induced cell death, which could have implications for the treatment response of HR-deficient tumours. PMID:25053826

Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks.

PubMed

Sotiriou, Sotirios K; Kamileri, Irene; Lugli, Natalia; Evangelou, Konstantinos; Da-Ré, Caterina; Huber, Florian; Padayachy, Laura; Tardy, Sebastien; Nicati, Noemie L; Barriot, Samia; Ochs, Fena; Lukas, Claudia; Lukas, Jiri; Gorgoulis, Vassilis G; Scapozza, Leonardo; Halazonetis, Thanos D

2016-12-15

Human cancers are characterized by the presence of oncogene-induced DNA replication stress (DRS), making them dependent on repair pathways such as break-induced replication (BIR) for damaged DNA replication forks. To better understand BIR, we performed a targeted siRNA screen for genes whose depletion inhibited G1 to S phase progression when oncogenic cyclin E was overexpressed. RAD52, a gene dispensable for normal development in mice, was among the top hits. In cells in which fork collapse was induced by oncogenes or chemicals, the Rad52 protein localized to DRS foci. Depletion of Rad52 by siRNA or knockout of the gene by CRISPR/Cas9 compromised restart of collapsed forks and led to DNA damage in cells experiencing DRS. Furthermore, in cancer-prone, heterozygous APC mutant mice, homozygous deletion of the Rad52 gene suppressed tumor growth and prolonged lifespan. We therefore propose that mammalian RAD52 facilitates repair of collapsed DNA replication forks in cancer cells. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

Why is SMOS Drier than the South Fork In-situ Soil Moisture Network?

NASA Astrophysics Data System (ADS)

Walker, V. A.; Hornbuckle, B. K.; Cosh, M. H.

2014-12-01

Global maps of near-surface soil moisture are currently being produced by the European Space Agency's Soil Moisture and Ocean Salinity (SMOS) satellite mission at 40 km. Within the next few months NASA's Soil Moisture Active Passive (SMAP) satellite mission will begin producing observations of near-surface soil moisture at 10 km. Near-surface soil moisture is the water content of the first 3 to 5 cm of the soil. Observations of near-surface soil moisture are expected to improve weather and climate forecasts. These satellite observations must be validated. We define validation as determining the space/time statistical characteristics of the uncertainty. A standard that has been used for satellite validation is in-situ measurements of near-surface soil moisture made with a network of sensors spanning the extent of a satellite footprint. Such a network of sensors has been established in the South Fork of the Iowa River in Central Iowa by the USDA ARS. Our analysis of data in 2013 indicates that SMOS has a dry bias: SMOS near-surface soil moisture is between 0.05 to 0.10 m^3m^{-3} lower than what is observed by the South Fork network. A dry bias in SMOS observations has also been observed in other regions of North America. There are many possible explanations for this difference: underestimation of vegetation, or soil surface roughness; undetected radio frequency interference (RFI); a retrieval model that is not appropriate for agricultural areas; or the use of an incorrect surface temperature in the retrieval process. We will begin our investigation by testing this last possibility: that SMOS is using a surface temperature that is too low which results in a drier soil moisture that compensates for this error. We will present a comparison of surface temperatures from the European Center for Medium-range Weather Forecasting (ECMWF) used to retrieve near-surface soil moisture from SMOS measurements of brightness temperature, and surface temperatures in the South Fork obtained from both tower and in-situ sensors. We will also use a long-term data set of tower and in-situ sensors collected in agricultural fields to develop a relationship between air temperature and the surface temperature relevant to the terrestrial microwave emission that is detected by SMOS.

Distinct functions of human RecQ helicases during DNA replication.

PubMed

Urban, Vaclav; Dobrovolna, Jana; Janscak, Pavel

2017-06-01

DNA replication is the most vulnerable process of DNA metabolism in proliferating cells and therefore it is tightly controlled and coordinated with processes that maintain genomic stability. Human RecQ helicases are among the most important factors involved in the maintenance of replication fork integrity, especially under conditions of replication stress. RecQ helicases promote recovery of replication forks being stalled due to different replication roadblocks of either exogenous or endogenous source. They prevent generation of aberrant replication fork structures and replication fork collapse, and are involved in proper checkpoint signaling. The essential role of human RecQ helicases in the genome maintenance during DNA replication is underlined by association of defects in their function with cancer predisposition. Copyright © 2016 Elsevier B.V. All rights reserved.

Navigating the socio-bio-geo-chemistry and engineering of nitrogen management in two illinois tile-drained watersheds.

PubMed

David, Mark B; Flint, Courtney G; Gentry, Lowell E; Dolan, Mallory K; Czapar, George F; Cooke, Richard A; Lavaire, Tito

2015-03-01

Reducing nitrate loads from corn and soybean, tile-drained, agricultural production systems in the Upper Mississippi River basin is a major challenge that has not been met. We evaluated a range of possible management practices from biophysical and social science perspectives that could reduce nitrate losses from tile-drained fields in the Upper Salt Fork and Embarras River watersheds of east-central Illinois. Long-term water quality monitoring on these watersheds showed that nitrate losses averaged 30.6 and 23.0 kg nitrate N ha yr (Embarras and Upper Salt Fork watersheds, respectively), with maximum nitrate concentrations between 14 and 18 mg N L. With a series of on-farm studies, we conducted tile monitoring to evaluate several possible nitrate reduction conservation practices. Fertilizer timing and cover crops reduced nitrate losses (30% reduction in a year with large nitrate losses), whereas drainage water management on one tile system demonstrated the problems with possible retrofit designs (water flowed laterally from the drainage water management tile to the free drainage system nearby). Tile woodchip bioreactors had good nitrate removal in 2012 (80% nitrate reduction), and wetlands had previously been shown to remove nitrate (45% reductions) in the Embarras watershed. Interviews and surveys indicated strong environmental concern and stewardship ethics among landowners and farmers, but the many financial and operational constraints that they operate under limited their willingness to adopt conservation practices that targeted nitrate reduction. Under the policy and production systems currently in place, large-scale reductions in nitrate losses from watersheds such as these in east-central Illinois will be difficult. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

Comparison of Three Biomass Sampling Techniques on Submersed Aquatic Plants in a Northern Tier Lake

DTIC Science & Technology

2010-07-01

distribution in 3 out of 14 species when comparing the box-core sampler and the rake method. These included forked duckweed (Lemna trisulca L, p...each site did not exhibit differences. These included coontail (p=0.2949), muskgrass (p=0.2746), American elodea (p=0.7622), forked duckweed (p...collected by the PVC-core sampler. These included coontail (p=0.000), chara (p=0.0219), American elodea (p=0.0061), forked duckweed (p=0.0000), najas (p

Grand Forks - East Grand Forks Urban Water Resources Study. East Grand Forks Flood Fight Manual.

DTIC Science & Technology

1981-07-01

wastewater management, and flood control) were identified, and a "plan of study" was developed. The plan of study outlined the general approach t~i -tiTd...three parts. Part 1 contains a general description and narrative on the need of the unit, Part 2 identifies the Unit Chief, Deputies, and Unit members...other units are discussed only in general terms. Future revisions will hopefully result in a happy medium between detailed specifics and generalities

Installation Restoration Program, Phase 2. Confirmation/Quantification, Stage 1. Kalispell Air Force Station, Montana

DTIC Science & Technology

1989-05-01

AFB Statement of Work. The Phase II Stage 1 investigation of Malmstrom AFB and two off-Base sites at Shelby and Brady, Montana are described in a...Flathead National Forest Headquarters in Kalispell and the Big Fork Ranger Station in Big Fork . Because there has been litigation involved with this...reviewed by phone. An interview at the spill site was held with Mr. William Pedersen, U.S. Forest Service Ranger for the Big Fork District. He personally

ANSYS simulation of the capacitance coupling of quartz tuning fork gyroscope

NASA Astrophysics Data System (ADS)

Zhang, Qing; Feng, Lihui; Zhao, Ke; Cui, Fang; Sun, Yu-nan

2013-12-01

Coupling error is one of the main error sources of the quartz tuning fork gyroscope. The mechanism of capacitance coupling error is analyzed in this article. Finite Element Method (FEM) is used to simulate the structure of the quartz tuning fork by ANSYS software. The voltage output induced by the capacitance coupling is simulated with the harmonic analysis and characteristics of electrical and mechanical parameters influenced by the capacitance coupling between drive electrodes and sense electrodes are discussed with the transient analysis.

An Inventory and Evaluation of Architectural and Engineering Resources of the Big South Fork National River and Recreation Area, Tennessee and Kentucky.

DTIC Science & Technology

1982-02-25

coordinated multidisciplinary study of both the architectural and engineering resources of the National Area. Both research b1 orientation and...South Fork just north of Rugby , and traveled through the site where Jamestown, Tennessee, now stands. A third trail, the Chickamauga Path, left the...Thomas Hughes (1881), the founder of the English colony of Rugby , Tennessee, described his neighbors in the Big South Fork area as mostly poor men

41 CFR 101-25.405 - Materials handling equipment.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 50 45 40 30 25 20 15 10 Straddle truck 15 50 50 50 45 45 45 40 40 35 35 30 25 20 15 10 Electric Fork... percentage of acquisition costs (years in use) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Gasoline Fork truck (2000 pounds to 6000 pounds) 8 50 45 40 30 25 20 15 10 Fork truck (over 6000 pounds) 10 50 45 40 35 30 25 20 15...

Spring constant of a tuning-fork sensor for dynamic force microscopy

PubMed Central

Lange, Manfred; Schmuck, Merlin; Schmidt, Nico; Möller, Rolf

2012-01-01

Summary We present an overview of experimental and numerical methods to determine the spring constant of a quartz tuning fork in qPlus configuration. The simple calculation for a rectangular cantilever is compared to the values obtained by the analysis of the thermal excitation and by the direct mechanical measurement of the force versus displacement. To elucidate the difference, numerical simulations were performed taking account of the real geometry including the glue that is used to mount the tuning fork. PMID:23365793

Water resources of the Fort Berthold Indian Reservation, west-central North Dakota

USGS Publications Warehouse

Cates, Steven W.; Macek-Rowland, Kathleen M.

1998-01-01

Water resources of the Fort Berthold Indian Reservation in west-central North Dakota occur as ground water in bedrock and buried-valley aquifers and as surface water in streams and Lake Sakakawea. The bedrock aquifers-the Fox Hills-Hell Creek, Tongue River, and Sentinel Butte store about 93 million acre-feet of water under the Reservation. The Fox Hills-Hell Creek aquifer is composed mainly of very fine to medium-grained sandstone and stores about 51 million acrefeet of water. Water levels in the aquifer declined from 1976 through 1992. The Tongue River aquifer is composed mainly of claystones and siltstones and has widely distributed pockets of sandstone or lignite layers. The aquifer stores about 24 million acre-feet of water. The Sentinel Butte aquifer is composed mainly of interbedded claystones, siltstones, shale, lignite, and sandstone and stores about 18 million acre-feet of water. Yields from the lignite beds are highly variable. Water in the aquifers was predominantly a sodium bicarbonate type. Mean dissolved solids concentrations were 1,530 milligrams per liter in water from the Fox Hills-Hell Creek aquifer, 2,110 milligrams per liter in water from the Tongue River aquifer, and 1,300 milligrams per liter in water from the Sentinel Butte aquifer. The East Fork Shell Creek, Shell Creek, White Shield, New Town, and Sanish aquifers occur within buried valleys and store about 1,414,000 acre-feet of water. The East Fork Shell Creek and Shell Creek aquifers are composed of sand and gravel lenses that are surrounded by less permeable till. Water in the East Fork Shell Creek aquifer is a sodium sulfate bicarbonate type, and water in the Shell Creek aquifer is a sodium bicarbonate sulfate type. Mean dissolved-solids concentrations were 3,220 milligrams per liter in water from the East Fork Shell Creek aquifer and 1,470 milligrams per liter in water from the Shell Creek aquifer.The White Shield aquifer is composed of very fine to coarse sand and fine to coarse gravel. Water in the aquifer varies from a sodium bicarbonate sulfate type to a mixed calcium magnesium sodium bicarbonate sulfate type. Mean dissolved-solids concentrations were 1,080 milligrams per liter in water from the eastern part of the aquifer and 1,430 milligrams per liter in water from the western part of the aquifer. Water levels in the western part of the aquifer rose during 1970-92. The New Town aquifer is composed of lenticular deposits of sand and gravel. Water in the aquifer is a calcium sodium bicarbonate sulfate type and had a mean dissolved-solids concentration of 1,390 milligrams per liter. Data indicate a close correspondence between ground-water levels and lake stage of Lake Sakakawea, implying a hydraulic connection between the aquifer and the lake.The Sanish aquifer is composed of sand, clayey sand, and thin gravels that are poorly cemented and highly permeable. Water in the aquifer is a mixed calcium magnesium bicarbonate sulfate type and had a mean dissolved-solids concentration of 1,350 milligrams per liter.Major streams on the Reservation are Bear Den Creek, Shell Creek, East Fork Shell Creek, Deepwater Creek, Moccasin Creek, and Squaw Creek. Mean streamflow for Bear Den Creek for June 1966 through September 1992 was 6.72 cubic feet per second. Mean streamflow for Shell Creek for September 1965 through September 1981 was 12.9 cubic feet per second. Streamflow measurements for East Fork Shell Creek for April 1990 through June 1991 ranged from zero to 3.65 cubic feet per second, measurements for Deepwater Creek for April 1990 through May 1991 ranged from zero to 4.28 cubic feet per second, measurements for Moccasin Creek for April 1990 through September 1992 ranged from zero to 7.07 cubic feet per second, and measurements for Squaw Creek for April 1990 through September 1992 ranged from zero to 4.22 cubic feet per second. Lake Sakakawea has a maximum surface area of 390,000 acres. The surface area is variable in relation to lake stage, which was unusually low during this study. The mean lake elevation for Lake Sakakawea for 1970-92 was 1,837.08 feet, and the mean lake elevation for 1990-92 was 1,821.14 feet.

What's in a goal? The role of motivational relevance in cognition and action.

PubMed

Eitam, Baruch; Higgins, E Tory

2014-04-01

We argue that it is possible to go beyond the "selfish goal" metaphor and make an even stronger case for the role of unconscious motivation in cognition and action. Through the relevance of a representation (ROAR) framework, we describe how not only value motivation, which relates to "selfish goals," but also truth motivation and control motivation impact cognition and action.

Paralysis at the Top of a Roaring Volcano: Israel and the Schooling of Palestinians in East Jerusalem

ERIC Educational Resources Information Center

Yair, Gad; Alayan, Samira

2009-01-01

Conflicts over East Jerusalem are often thought to reflect larger conflicts in the Middle East. In this article, the authors focus on schooling in East Jerusalem in order to provide a better appreciation of the protracted conflict in the area. This close examination of schooling in East Jerusalem can illuminate reasons for the political paralysis…

Educational Greenfield: A Critical Policy Analysis of Plans to Transform New Orleans Public Schools

ERIC Educational Resources Information Center

Torregano, Michelle Early; Shannon, Patrick

2009-01-01

New Orleans is known as a unique city. It is the birthplace of jazz, delicious food, and a "gumbo" of warm friendly people. On August 29, 2005, Hurricane Katrina roared ashore, leaving death and destruction in her wake. New Orleans Mayor C. Ray Nagin recognized that rebuilding the city of New Orleans would be a daunting task; one that he…

Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (85th, Miami, Florida, August 5-8, 2002). Magazine Division.

ERIC Educational Resources Information Center

2002

The Magazine Division of the proceedings contains the following 8 papers: "The Coverage of Prostate Cancer and Impotence in Four Magazines: 1991-2000" (W. Buzz Hoon); "A Content Analysis of Advertising Visuals in the Magazine Advertisements: The Roaring Twenties and the Great Depression" (Daechun An); "Do They 'Play Like…

The mouse that roared.

PubMed

Appleby, C

1996-02-20

They don't call the onslaught of managed care a revolution for nothing: everything is different now. Especially communications technology. The key tasks of managed care, from identifying best practices to coordinating physicians, are impossible when relying on paper records alone. That's why more health systems are depending on automated medical records. Though it's still not too late to catch up to stay competitive, now could be your last chance.

Structure of the replication fork in ultraviolet light-irradiated human cells.

PubMed Central

Cordeiro-Stone, M; Schumacher, R I; Meneghini, R

1979-01-01

The DNA extracted from xeroderma pigmentosum human fibroblasts previously irradiated with 12.5 J/m2 of UV light and pulse-labeled for 45 min with radioactive and (or) heavy precursors, was used to determine the structural characteristics of the replication fork. Density equilibrium centrifugation experiments showed that a fork moved 6 micrometer in 45 min and bypassed 3 pyrimidine dimers in both strands. The same length was covered in 15-20 min in control cells. The delay in irradiated cells was apparently due to pyrimidine dimers acting as temporary blocks to the fork movement. Evidence for this interpretation comes from kinetics of incorporation of [3H]thymidine into DNA, which show that the time necessary to attain a new stable level of DNA synthesis in irradiated cells is equivalent to that required for the replication fork to cover the interdimer distance in one strand. On the other hand, the action of S1 nuclease on DNA synthesized soon after irradiation gives rise to a bimodal distribution in neutral sucrose gradients, one peak corresponding to 43 X 10(6) daltons and the other to 3 X 10(6) daltons. These two DNA species are generated by the attack of the S1 nuclease on single-stranded regions associated with the replication fork. A possible explanation for these results is given by a model according to which there is a delayed bypass of the dimer in the leading strand and the appearance of gaps opposite pyrimidine dimers in the lagging strand, as a direct consequence of the discontinuous mode of DNA replication. In terms of the model, the DNA of 43 X 10(6) daltons corresponds to the leading strand, linked to the unreplicated branch of the forks, whereas the piece of 3 X 10(6) daltons is the intergap DNA coming from the lagging strand. Pulse and chase experiments reveal that the low molecular weight DNA grows in a pattern that suggests that more than one gap may be formed per replication fork. PMID:233582

Structure of the replication fork in ultraviolet light-irradiated human cells.

PubMed

Cordeiro-Stone, M; Schumacher, R I; Meneghini, R

1979-08-01

The DNA extracted from xeroderma pigmentosum human fibroblasts previously irradiated with 12.5 J/m2 of UV light and pulse-labeled for 45 min with radioactive and (or) heavy precursors, was used to determine the structural characteristics of the replication fork. Density equilibrium centrifugation experiments showed that a fork moved 6 micrometer in 45 min and bypassed 3 pyrimidine dimers in both strands. The same length was covered in 15-20 min in control cells. The delay in irradiated cells was apparently due to pyrimidine dimers acting as temporary blocks to the fork movement. Evidence for this interpretation comes from kinetics of incorporation of [3H]thymidine into DNA, which show that the time necessary to attain a new stable level of DNA synthesis in irradiated cells is equivalent to that required for the replication fork to cover the interdimer distance in one strand. On the other hand, the action of S1 nuclease on DNA synthesized soon after irradiation gives rise to a bimodal distribution in neutral sucrose gradients, one peak corresponding to 43 X 10(6) daltons and the other to 3 X 10(6) daltons. These two DNA species are generated by the attack of the S1 nuclease on single-stranded regions associated with the replication fork. A possible explanation for these results is given by a model according to which there is a delayed bypass of the dimer in the leading strand and the appearance of gaps opposite pyrimidine dimers in the lagging strand, as a direct consequence of the discontinuous mode of DNA replication. In terms of the model, the DNA of 43 X 10(6) daltons corresponds to the leading strand, linked to the unreplicated branch of the forks, whereas the piece of 3 X 10(6) daltons is the intergap DNA coming from the lagging strand. Pulse and chase experiments reveal that the low molecular weight DNA grows in a pattern that suggests that more than one gap may be formed per replication fork.

[History of the tuning fork. III: On the way to quantitative pure-tone measurement. Pictures from the history of otorhinolaryngology, represented by instruments from the collection of the Ingolstadt German Medical History Museum].

PubMed

Feldmann, H

1997-07-01

Weber's and Rinne's tuning-fork tests were for a long time considered unreliable, as they often seemed to yield inconsistent results. The sources of error were manifold and lay in the fields of physics, physiology, pathophysiology, and psychology. When the problems came to be understood, more sophisticated instruments and techniques were developed. The prongs of the tuning fork were fitted with clamps to deaden overtones when it was put into vibration (Politzer 1870). By shifting the clamps along the prongs the tone of the tuning fork could be varied in a range up to one octave (Könlg 1878). A knob of hom or metal was fixed to the end of the shaft to ensure a good coupling to the skull when testing bone conduction (Lucae 1886). A small hammer fixed to the shaft and driven by a spring would activate the tuning fork with reproducible strength (Lucae 1899). A wedge-shaped figure drawn on the lateral surface of the clamps would allow one to optically control the amplitude of vibration (Gradenigo 1899). The time during which a patient hears the tuning fork after it has been struck as compared to that of a normal hearing subject was measured as parameter of hearing acutiy (v. Conta 1864). A number of tuning forks at intervals of one octave each were assembled in sets to cover the whole frequency range of hearing. The most sophisticated example of these sets was the Bezold-Edelmann continuous tone series (1894). It comprised ten tuning forks with sliding clamps, two pipes of the organ type, and a Galton whistle. With this instrumentation it was possible to test the whole range of hearing. The results of testing the hearing via air conduction and bone conduction measured in duration and calculated as percentage of normal hearing were presented in charts (Hartmann 1885, Gradenigo 1893) which can be considered precursors of modern audiograms. The evolution of these instruments and methods is described in detail and illustrated by exhibits from the museum.

Recharge of shallow aquifers through two ephemeral-stream channels in northeastern Wyoming, 1982-1983

USGS Publications Warehouse

Lenfest, L.W.

1987-01-01

Quantifying the recharge from ephemeral streams to alluvial and bedrock aquifers will help evaluate the effects of surface mining on alluvial valley floors in Wyoming. Two stream reaches were chosen for study in the Powder River basin. One reach was located along the North Fork Dry Fork Cheyenne River near Glenrock, Wyoming, and the other reach was located along Black Thunder Creek near Hampshire, Wyoming. The reach along the North Fork Dry Fork Cheyenne River was instrumented with 3 gaging stations to measure streamflow and with 6 observation wells to measure groundwater level fluctuations in alluvial and bedrock aquifers in response to streamflow. The 3 streamflow gaging stations were located within the 2.5-mi study reach to measure the approximate gain or loss of discharge along the reach. Computed streamflow losses ranged from 0.43 acre-ft/mi on July 9 , 1982, to 1.44 acre-ft/mi on August 9, 1982. The observation wells completed only in the alluvial aquifer were dry during flow in the North Fork Dry Fork Cheyenne River, whereas water levels in half of the observation wells completed in the bedrock aquifers or the alluvial and bedrock aquifers rose in response to flow in the North Fork Dry Fork Cheyenne River. Groundwater recharge on August 9, 1982, was calculated using a convolution technique using groundwater levels at the upstream site and was estimated to be 26.5 acre-ft/mi. The reach along Black Thunder Creek was instrumented with one gaging station to measure streamflow and with 4 observation wells to measure water level response in alluvial and bedrock aquifers to streamflow. Recharge to the alluvial aquifer from flow in Black Thunder Creek ranged from 3.56 to 12.4 acre-ft/mi. The recharge was estimated using the convolution technique using water level measurements in the observation wells completed in the alluvial aquifer. Water level measurements in the observation wells indicated water level rises in the alluvial and bedrock aquifers in response to flow in Black Thunder Creek. (Author 's abstract)

14. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM TOWARD SETTLING BASIN, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM TOWARD SETTLING BASIN, SHOWING RIGHT FORK TO BYPASS, LEFT FORK TO BASIN - Electron Hydroelectric Project, Along Puyallup River, Electron, Pierce County, WA

77 FR 41897 - Airworthiness Directives; PZL Swidnik S.A. Helicopters

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-17

... control system hydraulic actuator fork end. These actions are intended to prevent rotation of the... locking of the longitudinal control system hydraulic actuator fork end. This condition, if not detected...

Little Known Facts about the Common Tuning Fork.

ERIC Educational Resources Information Center

Ong, P. P.

2002-01-01

Explains the physical principles of the tuning fork which has a common use in teaching laboratories. Includes information on its vibration, frequency of vibration, elasticity, and reasons for having two prongs. (YDS)

NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection

PubMed Central

Abeyta, Antonio; Castella, Maria; Jacquemont, Celine; Taniguchi, Toshiyasu

2017-01-01

ABSTRACT Proteins essential for homologous recombination play a pivotal role in the repair of DNA double strand breaks, DNA inter-strand crosslinks and replication fork stability. Defects in homologous recombination also play a critical role in the development of cancer and the sensitivity of these cancers to chemotherapy. RAD51, an essential factor for homologous recombination and replication fork protection, accumulates and forms immunocytochemically detectable nuclear foci at sites of DNA damage. To identify kinases that may regulate RAD51 localization to sites of DNA damage, we performed a human kinome siRNA library screen, using DNA damage-induced RAD51 foci formation as readout. We found that NEK8, a NIMA family kinase member, is required for efficient DNA damage-induced RAD51 foci formation. Interestingly, knockout of Nek8 in murine embryonic fibroblasts led to cellular sensitivity to the replication inhibitor, hydroxyurea, and inhibition of the ATR kinase. Furthermore, NEK8 was required for proper replication fork protection following replication stall with hydroxyurea. Loading of RAD51 to chromatin was decreased in NEK8-depleted cells and Nek8-knockout cells. Single-molecule DNA fiber analyses revealed that nascent DNA tracts were degraded in the absence of NEK8 following treatment with hydroxyurea. Consistent with this, Nek8-knockout cells showed increased chromosome breaks following treatment with hydroxyurea. Thus, NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51. PMID:27892797

NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection.

PubMed

Abeyta, Antonio; Castella, Maria; Jacquemont, Celine; Taniguchi, Toshiyasu

2017-02-16

Proteins essential for homologous recombination play a pivotal role in the repair of DNA double strand breaks, DNA inter-strand crosslinks and replication fork stability. Defects in homologous recombination also play a critical role in the development of cancer and the sensitivity of these cancers to chemotherapy. RAD51, an essential factor for homologous recombination and replication fork protection, accumulates and forms immunocytochemically detectable nuclear foci at sites of DNA damage. To identify kinases that may regulate RAD51 localization to sites of DNA damage, we performed a human kinome siRNA library screen, using DNA damage-induced RAD51 foci formation as readout. We found that NEK8, a NIMA family kinase member, is required for efficient DNA damage-induced RAD51 foci formation. Interestingly, knockout of Nek8 in murine embryonic fibroblasts led to cellular sensitivity to the replication inhibitor, hydroxyurea, and inhibition of the ATR kinase. Furthermore, NEK8 was required for proper replication fork protection following replication stall with hydroxyurea. Loading of RAD51 to chromatin was decreased in NEK8-depleted cells and Nek8-knockout cells. Single-molecule DNA fiber analyses revealed that nascent DNA tracts were degraded in the absence of NEK8 following treatment with hydroxyurea. Consistent with this, Nek8-knockout cells showed increased chromosome breaks following treatment with hydroxyurea. Thus, NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51.

Chromosomal Integrity after UV Irradiation Requires FANCD2-Mediated Repair of Double Strand Breaks

PubMed Central

Federico, María Belén; Vallerga, María Belén; Radl, Analía; Paviolo, Natalia Soledad; Bocco, José Luis; Di Giorgio, Marina; Soria, Gastón; Gottifredi, Vanesa

2016-01-01

Fanconi Anemia (FA) is a rare autosomal recessive disorder characterized by hypersensitivity to inter-strand crosslinks (ICLs). FANCD2, a central factor of the FA pathway, is essential for the repair of double strand breaks (DSBs) generated during fork collapse at ICLs. While lesions different from ICLs can also trigger fork collapse, the contribution of FANCD2 to the resolution of replication-coupled DSBs generated independently from ICLs is unknown. Intriguingly, FANCD2 is readily activated after UV irradiation, a DNA-damaging agent that generates predominantly intra-strand crosslinks but not ICLs. Hence, UV irradiation is an ideal tool to explore the contribution of FANCD2 to the DNA damage response triggered by DNA lesions other than ICL repair. Here we show that, in contrast to ICL-causing agents, UV radiation compromises cell survival independently from FANCD2. In agreement, FANCD2 depletion does not increase the amount of DSBs generated during the replication of UV-damaged DNA and is dispensable for UV-induced checkpoint activation. Remarkably however, FANCD2 protects UV-dependent, replication-coupled DSBs from aberrant processing by non-homologous end joining, preventing the accumulation of micronuclei and chromatid aberrations including non-homologous chromatid exchanges. Hence, while dispensable for cell survival, FANCD2 selectively safeguards chromosomal stability after UV-triggered replication stress. PMID:26765540

Chromosomal Integrity after UV Irradiation Requires FANCD2-Mediated Repair of Double Strand Breaks.

PubMed

Federico, María Belén; Vallerga, María Belén; Radl, Analía; Paviolo, Natalia Soledad; Bocco, José Luis; Di Giorgio, Marina; Soria, Gastón; Gottifredi, Vanesa

2016-01-01

Fanconi Anemia (FA) is a rare autosomal recessive disorder characterized by hypersensitivity to inter-strand crosslinks (ICLs). FANCD2, a central factor of the FA pathway, is essential for the repair of double strand breaks (DSBs) generated during fork collapse at ICLs. While lesions different from ICLs can also trigger fork collapse, the contribution of FANCD2 to the resolution of replication-coupled DSBs generated independently from ICLs is unknown. Intriguingly, FANCD2 is readily activated after UV irradiation, a DNA-damaging agent that generates predominantly intra-strand crosslinks but not ICLs. Hence, UV irradiation is an ideal tool to explore the contribution of FANCD2 to the DNA damage response triggered by DNA lesions other than ICL repair. Here we show that, in contrast to ICL-causing agents, UV radiation compromises cell survival independently from FANCD2. In agreement, FANCD2 depletion does not increase the amount of DSBs generated during the replication of UV-damaged DNA and is dispensable for UV-induced checkpoint activation. Remarkably however, FANCD2 protects UV-dependent, replication-coupled DSBs from aberrant processing by non-homologous end joining, preventing the accumulation of micronuclei and chromatid aberrations including non-homologous chromatid exchanges. Hence, while dispensable for cell survival, FANCD2 selectively safeguards chromosomal stability after UV-triggered replication stress.

Effectiveness of a redesigned water diversion using rock vortex weirs to enhance longitudinal connectivity for small Salmonids

USGS Publications Warehouse

Martens, Kyle D.; Connolly, Patrick J.

2010-01-01

For nearly 100 years, water diversions have affected fish passage in Beaver Creek, a tributary of the lower Methow River in north-central Washington State. From 2000 to 2004, four dam-style water diversions were replaced with a series of rock vortex weirs (RVWs). The weirs were designed to allow fish passage while maintaining the ability to divert water into irrigation canals. We observed the new appearance of three species (juvenile Chinook salmon Oncorhynchus tshawytscha, juvenile coho salmon O. kisutch, and mountain whitefish Prosopium williamsoni) upstream of the RVWs, indicating successful restoration of longitudinal connectivity. We used passive integrated transponder (PIT) tags and instream PIT tag interrogation systems during 2004–2007 to evaluate upstream passage of small salmonids (<240 mm fork length) through one series of RVWs. We documented 109 upstream passage events by small salmonids through the series of RVWs; most of the events (81%) involved passage of rainbow trout O. mykiss or juvenile steelhead (anadromous rainbow trout). Small rainbow trout or steelhead ranging from 86 to 238 mm (adjusted fork length) were able to pass upstream through the RVWs, although a delay in fish passage at discharges below 0.32 m3/s was detected in comparison with nearby control sections.

Chloride control and monitoring program in the Wichita River Basin, Texas, 1996-2009

USGS Publications Warehouse

Haynie, M.M.; Burke, G.F.; Baldys, Stanley

2011-01-01

Water resources of the Wichita River Basin in north-central Texas are vital to the water users in Wichita Falls, Tex., and surrounding areas. The Wichita River Basin includes three major forks of the Wichita River upstream from Lake Kemp, approximately 50 miles southwest of Wichita Falls, Tex. The main stem of the Wichita River is formed by the confluence of the North Wichita River and Middle Fork Wichita River upstream from Truscott Brine Lake. The confluence of the South Wichita River with the Wichita River is northwest of Seymour, Tex. (fig. 1). Waters from the Wichita River Basin, which is part of the Red River Basin, are characterized by high concentrations of chloride and other salinity-related constituents from salt springs and seeps (hereinafter salt springs) in the upper reaches of the basin. These salt springs have their origins in the Permian Period when the Texas Panhandle and western Oklahoma areas were covered by a broad shallow sea. Over geologic time, evaporation of the shallow seas resulted in the formation of salt deposits, which today are part of the geologic formations underlying the area. Groundwater in these formations is characterized by high chloride concentrations from these salt deposits, and some of this groundwater is discharged by the salt springs into the Wichita River.

Water-quality trends and constituent-transport analysis for selected sampling sites in the Milltown Reservoir/Clark Fork River Superfund Site in the upper Clark Fork Basin, Montana, water years 1996–2015

USGS Publications Warehouse

Sando, Steven K.; Vecchia, Aldo V.

2016-07-20

During the extended history of mining in the upper Clark Fork Basin in Montana, large amounts of waste materials enriched with metallic contaminants (cadmium, copper, lead, and zinc) and the metalloid trace element arsenic were generated from mining operations near Butte and milling and smelting operations near Anaconda. Extensive deposition of mining wastes in the Silver Bow Creek and Clark Fork channels and flood plains had substantial effects on water quality. Federal Superfund remediation activities in the upper Clark Fork Basin began in 1983 and have included substantial remediation near Butte and removal of the former Milltown Dam near Missoula. To aid in evaluating the effects of remediation activities on water quality, the U.S. Geological Survey began collecting streamflow and water-quality data in the upper Clark Fork Basin in the 1980s.Trend analysis was done on specific conductance, selected trace elements (arsenic, copper, and zinc), and suspended sediment for seven sampling sites in the Milltown Reservoir/Clark Fork River Superfund Site for water years 1996–2015. The most upstream site included in trend analysis is Silver Bow Creek at Warm Springs, Montana (sampling site 8), and the most downstream site is Clark Fork above Missoula, Montana (sampling site 22), which is just downstream from the former Milltown Dam. Water year is the 12-month period from October 1 through September 30 and is designated by the year in which it ends. Trend analysis was done by using a joint time-series model for concentration and streamflow. To provide temporal resolution of changes in water quality, trend analysis was conducted for four sequential 5-year periods: period 1 (water years 1996–2000), period 2 (water years 2001–5), period 3 (water years 2006–10), and period 4 (water years 2011–15). Because of the substantial effect of the intentional breach of Milltown Dam on March 28, 2008, period 3 was subdivided into period 3A (October 1, 2005–March 27, 2008) and period 3B (March 28, 2008–September 30, 2010) for the Clark Fork above Missoula (sampling site 22). Trend results were considered statistically significant when the statistical probability level was less than 0.01.In conjunction with the trend analysis, estimated normalized constituent loads (hereinafter referred to as “loads”) were calculated and presented within the framework of a constituent-transport analysis to assess the temporal trends in flow-adjusted concentrations (FACs) in the context of sources and transport. The transport analysis allows assessment of temporal changes in relative contributions from upstream source areas to loads transported past each reach outflow.Trend results indicate that FACs of unfiltered-recoverable copper decreased at the sampling sites from the start of period 1 through the end of period 4; the decreases ranged from large for one sampling site (Silver Bow Creek at Warm Springs [sampling site 8]) to moderate for two sampling sites (Clark Fork near Galen, Montana [sampling site 11] and Clark Fork above Missoula [sampling site 22]) to small for four sampling sites (Clark Fork at Deer Lodge, Montana [sampling site 14], Clark Fork at Goldcreek, Montana [sampling site 16], Clark Fork near Drummond, Montana [sampling site 18], and Clark Fork at Turah Bridge near Bonner, Montana [sampling site 20]). For period 4 (water years 2011–15), the most notable changes indicated for the Milltown Reservoir/Clark Fork River Superfund Site were statistically significant decreases in FACs and loads of unfiltered-recoverable copper for sampling sites 8 and 22. The period 4 changes in FACs of unfiltered-recoverable copper for all other sampling sites were not statistically significant.Trend results indicate that FACs of unfiltered-recoverable arsenic decreased at the sampling sites from period 1 through period 4 (water years 1996–2015); the decreases ranged from minor (sampling sites 8–20) to small (sampling site 22). For period 4 (water years 2011–15), the most notable changes indicated for the Milltown Reservoir/Clark Fork River Superfund Site were statistically significant decreases in FACs and loads of unfiltered-recoverable arsenic for sampling site 8 and near statistically significant decreases for sampling site 22. The period 4 changes in FACs of unfiltered-recoverable arsenic for all other sampling sites were not statistically significant.Trend results indicate that FACs of suspended sediment decreased at the sampling sites from period 1 through period 4 (water years 1996–2015); the decreases ranged from moderate (sampling site 8) to small (sampling sites 11–22). For period 4 (water years 2011–15), the changes in FACs of suspended sediment were not statistically significant for any sampling sites.The reach of the Clark Fork from Galen to Deer Lodge is a large source of metallic contaminants and suspended sediment, which strongly affects downstream transport of those constituents. Mobilization of copper and suspended sediment from flood-plain tailings and the streambed of the Clark Fork and its tributaries within the reach results in a contribution of those constituents that is proportionally much larger than the contribution of streamflow from within the reach. Within the reach from Galen to Deer Lodge, unfiltered-recoverable copper loads increased by a factor of about 4 and suspended-sediment loads increased by a factor of about 5, whereas streamflow increased by a factor of slightly less than 2. For period 4 (water years 2011–15), unfiltered-recoverable copper and suspended-sediment loads sourced from within the reach accounted for about 41 and 14 percent, respectively, of the loads at Clark Fork above Missoula (sampling site 22), whereas streamflow sourced from within the reach accounted for about 4 percent of the streamflow at sampling site 22. During water years 1996–2015, decreases in FACs and loads of unfiltered-recoverable copper and suspended sediment for the reach generally were proportionally smaller than for most other reaches.Unfiltered-recoverable copper loads sourced within the reaches of the Clark Fork between Deer Lodge and Turah Bridge near Bonner (just upstream from the former Milltown Dam) were proportionally smaller than contributions of streamflow sourced from within the reaches; these reaches contributed proportionally much less to copper loading in the Clark Fork than the reach between Galen and Deer Lodge. Although substantial decreases in FACs and loads of unfiltered-recoverable copper and suspended sediment were indicated for Silver Bow Creek at Warm Springs (sampling site 8), those substantial decreases were not translated to downstream reaches between Deer Lodge and Turah Bridge near Bonner. The effect of the reach of the Clark Fork from Galen to Deer Lodge as a large source of copper and suspended sediment, in combination with little temporal change in those constituents for the reach, contributes to this pattern.With the removal of the former Milltown Dam in 2008, substantial amounts of contaminated sediments that remained in the Clark Fork channel and flood plain in reach 9 (downstream from Turah Bridge near Bonner) became more available for mobilization and transport than before the dam removal. After the removal of the former Milltown Dam, the Clark Fork above Missoula (sampling site 22) had statistically significant decreases in FACs of unfiltered-recoverable copper in period 3B (March 28, 2008, through water year 2010) that continued in period 4 (water years 2011–15). Also, decreases in FACs of unfiltered-recoverable arsenic and suspended sediment were indicated for period 4 at this site. The decrease in FACs of unfiltered-recoverable copper for sampling site 22 during period 4 was proportionally much larger than the decrease for the Clark Fork at Turah Bridge near Bonner (sampling site 20). Net mobilization of unfiltered-recoverable copper and arsenic from sources within reach 9 are smaller for period 4 than for period 1 when the former Milltown Dam was in place, providing evidence that contaminant source materials have been substantially reduced in reach 9.

Von Economo Neurons and Fork Cells: A Neurochemical Signature Linked to Monoaminergic Function.

PubMed

Dijkstra, Anke A; Lin, Li-Chun; Nana, Alissa L; Gaus, Stephanie E; Seeley, William W

2018-01-01

The human anterior cingulate and frontoinsular cortices are distinguished by 2 unique Layer 5 neuronal morphotypes, the von Economo neurons (VENs) and fork cells, whose biological identity remains mysterious. Insights could impact research on diverse neuropsychiatric diseases to which these cells have been linked. Here, we leveraged the Allen Brain Atlas to evaluate mRNA expression of 176 neurotransmitter-related genes and identified vesicular monoamine transporter 2 (VMAT2), gamma-aminobutyric acid (GABA) receptor subunit θ (GABRQ), and adrenoreceptor α-1A (ADRA1A) expression in human VENs, fork cells, and a minority of neighboring Layer 5 neurons. We confirmed these results using immunohistochemistry or in situ hybridization. VMAT2 and GABRQ expression was absent in mouse cerebral cortex. Although VMAT2 is known to package monoamines into synaptic vesicles, in VENs and fork cells its expression occurs in the absence of monoamine-synthesizing enzymes or reuptake transporters. Thus, VENs and fork cells may possess a novel, uncharacterized mode of cortical monoaminergic function that distinguishes them from most other mammalian Layer 5 neurons. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Numerical performance analysis of quartz tuning fork-based force sensors

NASA Astrophysics Data System (ADS)

Dagdeviren, Omur E.; Schwarz, Udo D.

2017-01-01

Quartz tuning fork-based force sensors where one prong is immobilized onto a holder while the other one is allowed to oscillate freely (‘qPlus’ configuration) are in widespread use for high-resolution scanning probe microscopy applications. Due to the small size of the tuning forks (≈3 mm) and the complexity of the sensor assemblies, the reliable and repeatable manufacturing of the sensors has been challenging. In this paper, we investigate the contribution of the amount and location of the epoxy glue used to attach the tuning fork to its holder on the sensor’s performance. Towards this end, we use finite element analysis to model the entire sensor assembly and to perform static and dynamic numerical simulations. Our analysis reveals that increasing the thickness of the epoxy layer between prong and holder results in a decrease of the sensor’s spring constant, eigenfrequency, and quality factor while showing an increasing deviation from oscillation in its primary modal shape. Adding epoxy at the sides of the tuning fork also leads to a degradation of the quality factor even though in this case, spring constant and eigenfrequency rise in tandem with a lessening of the deviation from its ideal modal shape.

Effects of acid mine drainage on the stream ecosystem of the east fork of the Obey River, Tennessee

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nichols, L.E.; Bulow, F.L.

1973-01-01

The stream ecosystem of the east fork of the Obey River, Tennessee was studied from January through December 1970. Emphasis centered on water quality, macroinvertebrates, fish and aquatic flora affected by acid mine drainage. Two control stations were established within the study area, one located below the zone of pollution. A reservoir station was established to detect any neutralization occurring within Dale Hollow Reservoir below the confluence of the east fork and west fork. An area approximately 40 miles in length was found to be severely degraded by acid mine drainage. Limited macroinvertebrate populations existed within this region. Chironomus andmore » Sialis were the predominate benthic indicator organisms present in the polluted zone. Euglena mutabilis was the most abundant representative organism of the aquatic flora. This organism was found to be characteristic of acid mine pollution. Fish were recovered above and below, but not within, the zone of pollution. Fish recovered were characteristic of the type of habitat in which they were collected, being either typical stream or reservoir fish. A fish kill occurred in the east fork embayment of Dale Hollow on August 15, 1970 from acid mine drainage discharged upstream.« less

The annealing helicase and branch migration activities of Drosophila HARP.

PubMed

Kassavetis, George A; Kadonaga, James T

2014-01-01

HARP (SMARCAL1, MARCAL1) is an annealing helicase that functions in the repair and restart of damaged DNA replication forks through its DNA branch migration and replication fork regression activities. HARP is conserved among metazoans. HARP from invertebrates differs by the absence of one of the two HARP-specific domain repeats found in vertebrates. The annealing helicase and branch migration activity of invertebrate HARP has not been documented. We found that HARP from Drosophila melanogaster retains the annealing helicase activity of human HARP, the ability to disrupt D-loops and to branch migrate Holliday junctions, but fails to regress model DNA replication fork structures. A comparison of human and Drosophila HARP on additional substrates revealed that both HARPs are competent in branch migrating a bidirectional replication bubble composed of either DNA:DNA or RNA:DNA hybrid. Human, but not Drosophila, HARP is also capable of regressing a replication fork structure containing a highly stable poly rG:dC hybrid. Persistent RNA:DNA hybrids in vivo can lead to replication fork arrest and genome instability. The ability of HARP to strand transfer hybrids may signify a hybrid removal function for this enzyme, in vivo.

Accurate aging of juvenile salmonids using fork lengths

USGS Publications Warehouse

Sethi, Suresh; Gerken, Jonathon; Ashline, Joshua

2017-01-01

Juvenile salmon life history strategies, survival, and habitat interactions may vary by age cohort. However, aging individual juvenile fish using scale reading is time consuming and can be error prone. Fork length data are routinely measured while sampling juvenile salmonids. We explore the performance of aging juvenile fish based solely on fork length data, using finite Gaussian mixture models to describe multimodal size distributions and estimate optimal age-discriminating length thresholds. Fork length-based ages are compared against a validation set of juvenile coho salmon, Oncorynchus kisutch, aged by scales. Results for juvenile coho salmon indicate greater than 95% accuracy can be achieved by aging fish using length thresholds estimated from mixture models. Highest accuracy is achieved when aged fish are compared to length thresholds generated from samples from the same drainage, time of year, and habitat type (lentic versus lotic), although relatively high aging accuracy can still be achieved when thresholds are extrapolated to fish from populations in different years or drainages. Fork length-based aging thresholds are applicable for taxa for which multiple age cohorts coexist sympatrically. Where applicable, the method of aging individual fish is relatively quick to implement and can avoid ager interpretation bias common in scale-based aging.

CANVAS FINISH TO REMOVE FLOAT MARKS. View is to the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

CANVAS FINISH TO REMOVE FLOAT MARKS. View is to the northwest of deck finishing operations - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

27 CFR 9.65 - North Fork of Roanoke.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the North... and 697 in Roanoke County. (2) Then the boundary follows State Route 697 northeast over Crawford Ridge...

27 CFR 9.65 - North Fork of Roanoke.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the North... and 697 in Roanoke County. (2) Then the boundary follows State Route 697 northeast over Crawford Ridge...

14. View to southwest. View through truss along centerline from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. View to southwest. View through truss along centerline from below deck. (65mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

20. View to southeast. Aerial view of bridge in setting; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. View to southeast. Aerial view of bridge in setting; downstream side. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

COUNTERWEIGHT FOOTINGS ON EAST SIDE. View is to the north ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

COUNTERWEIGHT FOOTINGS ON EAST SIDE. View is to the north from the old suspension bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

13. View to northeast. View along centerline from Humboldt County ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. View to northeast. View along centerline from Humboldt County side of bridge. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

JIGGER STICK USED IN ERECTION. Shown on highline, direction of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

JIGGER STICK USED IN ERECTION. Shown on highline, direction of view is probably to the north - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

12. View to southwest. View along centerline from Trinity County ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. View to southwest. View along centerline from Trinity County side of bridge. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

11. View to southeast. More distant overview of bridge in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. View to southeast. More distant overview of bridge in setting; downstream side. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

26. MOORSE DRILL CABINET AND FORK ART FABRICATED AT SHOP ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. MOORSE DRILL CABINET AND FORK ART FABRICATED AT SHOP (L TO R)- LOOKING SOUTHEAST. - W. A. Young & Sons Foundry & Machine Shop, On Water Street along Monongahela River, Rices Landing, Greene County, PA

Concentrations and loads of cadmium, lead, and zinc measured near the peak of the 1999 snowmelt-runoff hydrographs for 42 water-quality stations, Coeur d'Alene River basin, Idaho

USGS Publications Warehouse

Woods, Paul F.

2000-01-01

The Coeur d’Alene River near Harrison transported 924 pounds of dissolved lead per day, of which 82.8 pounds came from the South Fork and 11.7 pounds from the North Fork. Only 10.2 percent of the load at Harrison was measured at the Pinehurst and Enaville stations; therefore, a substantial load of dissolved lead is being contributed downstream from the confluence of the North and South Forks.

Cultural Resource Investigation of the Grand Forks/East Grand Forks Urban Study and the East Grand Forks Flood Control Project,

DTIC Science & Technology

1981-04-01

and will not be impacted. Remarks: Level II. Father William Sherman, Pastor of St. Michael’s Church can be reached at St. Michael’s Church, 520 North...Trygg, J. William 1967 Composite Map of the Uni- % [ted States Land Surveyors’ L Original Plats and Field Notes, Minnesota Series, Sheet 20, Ely...Investigators/Years: Surveyors’ Original Plats and Notes, ’ 1872 Report/Reference: Trygg, J. William * 1967 Composite Map of United States Surveyors

Cultural Resources Reconnaissance for Proposed Flood Control Measures at Cramerton, Gaston County, North Carolina.

DTIC Science & Technology

1982-01-28

by the South Fork of the 6 I. .. . ... . .. . . . Catawba Piver. The South Fork is a major upper tributary of the Santee drainage system. :t rises in...streams in the survey area are first order tributaries . Several of the smaller streams are not depicted an the 1:24,000 topographic sheet. The South Fork...of i-ost.oid O’. Cultura aate’rial density in the s=r2eJ and deflated sample area is 0.35 piecei1.0 szuare meter. Anite quartz ncdules w€ere eroded

The Sound Field Around a Tuning Fork and the Role of a Resonance Box

NASA Astrophysics Data System (ADS)

Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

2015-02-01

Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: —Why is a bare tuning fork such a weak emitter of sound?—What is the role of the resonance box?—Where does energy connected with larger intensity of emitted acoustic waves come from?

Water-Quality, Bed-Sediment, and Biological Data (October 2006 through September 2007) and Statistical Summaries of Long-Term Data for Streams in the Clark Fork Basin, Montana

USGS Publications Warehouse

Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

2008-01-01

Water, bed sediment, and biota were sampled in streams from Butte to below Milltown Reservoir as part of a long-term monitoring program in the upper Clark Fork basin; additional water-quality samples were collected in the Clark Fork basin from sites near Milltown Reservoir downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water-quality samples were collected periodically at 22 sites from October 2006 through September 2007. Bed-sediment and biological samples were collected once at 12 sites during August 2007. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2006 through September 2007. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Turbidity was analyzed for samples collected at sites where seasonal daily values of turbidity were being determined. Nutrients also were analyzed in the supplemental water-quality samples. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for four sites, and seasonal daily values of turbidity were determined for five sites. Bed-sediment data include trace-element concentrations in the fine-grained fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

Leadership in elephants: the adaptive value of age

PubMed Central

McComb, Karen; Shannon, Graeme; Durant, Sarah M.; Sayialel, Katito; Slotow, Rob; Poole, Joyce; Moss, Cynthia

2011-01-01

The value of age is well recognized in human societies, where older individuals often emerge as leaders in tasks requiring specialized knowledge, but what part do such individuals play in other social species? Despite growing interest in how effective leadership might be achieved in animal social systems, the specific role that older leaders may play in decision-making has rarely been experimentally investigated. Here, we use a novel playback paradigm to demonstrate that in African elephants (Loxodonta africana), age affects the ability of matriarchs to make ecologically relevant decisions in a domain critical to survival—the assessment of predatory threat. While groups consistently adjust their defensive behaviour to the greater threat of three roaring lions versus one, families with younger matriarchs typically under-react to roars from male lions despite the severe danger they represent. Sensitivity to this key threat increases with matriarch age and is greatest for the oldest matriarchs, who are likely to have accumulated the most experience. Our study provides the first empirical evidence that individuals within a social group may derive significant benefits from the influence of an older leader because of their enhanced ability to make crucial decisions about predatory threat, generating important insights into selection for longevity in cognitively advanced social mammals. PMID:21411454

Distribution of biota in a stream polluted by acid mine-drainage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Warner, R.W.

1971-01-01

Acidic water draining from coal mines has severly restricted the diversity of biota inhabiting Roaring Creek, eastern West Virginia. Polluted reaches of the stream (median pH values ranging from 2.8 to 3.8) were inhabited by 3 to 12 genera of bottom-dwelling invertebrates and 10 to 19 species of periphytic algae. Invertebrates tolerant of the pollution included Sialis sp., chironomus plumosus and other Chironomidae, dytiscid beetles, and Ptilostomis sp. Predominant among the tolerant periphyton were Ulothrix tenerrima, Pinnularia termitina, Eunotia exigua, and Euglena mutabilis. Six other species of algae were tolerant of the acid mine-pollution, but were never numerous. Sections ofmore » Roaring Creek not severely polluted by acid drainage (pH medians of 4.5 or higher) supported diverse communities of 25 or more kinds of benthic animals and 27 or more species of periphytic algae. These stream reaches were inhibited by blackflies, crayfish, mayflies, stoneflies, and many species of caddisflies; these forms did not inhabit the more acidic stream reaches. Because of the complex and varying chemical composition of the acid mine-drainage, and also because of possible physical influences, measurements of pH values in the stream seemed to provide the most reliable, as well as unique, index of the effects of acid mine-drainage on aquatic life.« less

37. BRIDGE 115, SMITH RIVER MIDDLE FORK OREGON STATE HIGHWAY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

37. BRIDGE 1-15, SMITH RIVER MIDDLE FORK OREGON STATE HIGHWAY 199. JOSEPHINE COUNTY, OREGON. LOOKING SSW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

2. GENERAL VIEW OF BRIDGE FROM ROADBED WITH 4' RANGE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. GENERAL VIEW OF BRIDGE FROM ROADBED WITH 4' RANGE POLE NEAR NORTHWEST CORNER OF BRIDGE, LOOKING SOUTH - North Fork Bridge, Spans North Fork of White River at State Highway 5, Norfork, Baxter County, AR

PIER 2. View is to the northeast, looking from Pier ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PIER 2. View is to the northeast, looking from Pier 1 toward Pier 2 from beneath completed bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

3. View to southwest. Oblique view of downstream side of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. View to southwest. Oblique view of downstream side of bridge and west pier. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

2. View to east. Oblique view of downstream side of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. View to east. Oblique view of downstream side of bridge and east pier. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

9. View to northeast. Oblique view of upstream side of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. View to northeast. Oblique view of upstream side of bridge from approximately deck level. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

PLACING TOP CHORD CENTER PANEL. View to the northwest from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PLACING TOP CHORD CENTER PANEL. View to the northwest from the old suspension bridge. Chord members in place - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

17. View to west. Detail, connection point L2 (see plans), ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. View to west. Detail, connection point L2 (see plans), from below deck. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

35. CHARGING DOOR OF CUPOLA FORM LOFT, WITH FORKS FOR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. CHARGING DOOR OF CUPOLA FORM LOFT, WITH FORKS FOR FEEDING COKE, FOUNDRY BELOW-LOOKING NORTH. - W. A. Young & Sons Foundry & Machine Shop, On Water Street along Monongahela River, Rices Landing, Greene County, PA

Tuning Forks and Monitor Screens.

ERIC Educational Resources Information Center

Harrison, M. A. T.

2000-01-01

Defines the vibrations of a tuning fork against a computer monitor screen as a pattern that can illustrate or explain physical concepts like wave vibrations, wave forms, and phase differences. Presents background information and demonstrates the experiment. (Author/YDS)

6. VIEW FACING EAST ALONG NORTH FACE OF BRIDGE AT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. VIEW FACING EAST ALONG NORTH FACE OF BRIDGE AT CONSTRUCTION DETAILS OF WOOD RAILINGS AND STONE ABUTMENTS. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA

17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK THAT MIXED COFFEE BEANS AS THEY WERE HUSKED - Hacienda Cafetalera Santa Clara, Coffee Mill, KM 19, PR Route 372, Hacienda La Juanita, Yauco Municipio, PR

Chemical bond imaging using higher eigenmodes of tuning fork sensors in atomic force microscopy

NASA Astrophysics Data System (ADS)

Ebeling, Daniel; Zhong, Qigang; Ahles, Sebastian; Chi, Lifeng; Wegner, Hermann A.; Schirmeisen, André

2017-05-01

We demonstrate the ability of resolving the chemical structure of single organic molecules using non-contact atomic force microscopy with higher normal eigenmodes of quartz tuning fork sensors. In order to achieve submolecular resolution, CO-functionalized tips at low temperatures are used. The tuning fork sensors are operated in ultrahigh vacuum in the frequency modulation mode by exciting either their first or second eigenmode. Despite the high effective spring constant of the second eigenmode (on the order of several tens of kN/m), the force sensitivity is sufficiently high to achieve atomic resolution above the organic molecules. This is observed for two different tuning fork sensors with different tip geometries (small tip vs. large tip). These results represent an important step towards resolving the chemical structure of single molecules with multifrequency atomic force microscopy techniques where two or more eigenmodes are driven simultaneously.

Tectonic controls on large landslide complex: Williams Fork Mountains near Dillon, Colorado

USGS Publications Warehouse

Kellogg, K.S.

2001-01-01

An extensive (~ 25 km2) landslide complex covers a large area on the west side of the Williams Fork Mountains in central Colorado. The complex is deeply weathered and incised, and in most places geomorphic evidence of sliding (breakaways, hummocky topography, transverse ridges, and lobate distal zones) are no longer visible, indicating that the main mass of the slide has long been inactive. However, localized Holocene reactivation of the landslide deposits is common above the timberline (at about 3300 m) and locally at lower elevations. Clasts within the complex, as long as several tens of meters, are entirely of crystalline basement (Proterozoic gneiss and granitic rocks) from the hanging wall of the Laramide (Late Cretaceous to Early Tertiary), west-directed Williams Range thrust, which forms the western structural boundary of the Colorado Front Range. Late Cretaceous shale and sandstone compose most footwall rocks. The crystalline hanging-wall rocks are pervasively fractured or shattered, and alteration to clay minerals is locally well developed. Sackung structures (trenches or small-scale grabens and upslope-facing scarps) are common near the rounded crest of the range, suggesting gravitational spreading of the fractured rocks and oversteepening of the mountain flanks. Late Tertiary and Quaternary incision of the Blue River Valley, just west of the Williams Fork Mountains, contributed to the oversteepening. Major landslide movement is suspected during periods of deglaciation when abundant meltwater increased pore-water pressure in bedrock fractures. A fault-flexure model for the development of the widespread fracturing and weakening of the Proterozoic basement proposes that the surface of the Williams Range thrust contains a concave-downward flexure, the axis of which coincides approximately with the contact in the footwall between Proterozoic basement and mostly Cretaceous rocks. Movement of brittle, hanging-wall rocks through the flexure during Laramide deformation pervasively fractured the hanging-wall rocks. ?? 2001 Elsevier Science B.V. All rights reserved.

Evaluation of physicochemical and physical habitat associations for Cambarus callainus (Big Sandy crayfish), an imperilled crayfish endemic to the Central Appalachians

USGS Publications Warehouse

Loughman, Zachary J.; Welsh, Stuart A.; Sadecky, Nicole M.; Dillard, Zachary W.; Scott, R. Katie

2017-01-01

1. Crayfish represent one of the most imperilled animal groups on the planet. Habitat degradation, destruction and fragmentation, introduction of invasive crayfishes, and a lack of applied biological information have all been identified as agents thwarting crayfish conservation.2. Cambarus callainus was warranted federal protection by the United States Fish and Wildlife Service (USFWS) in April, 2016. As part of the USFWS listing procedure, a survey for C. callainus in the Big Sandy River catchment was conducted to determine points of occurrence with a secondary objective of determining reach level physical habitat and physicochemical correlates of C. callainus presence and absence.3. At each site, physicochemical and physical habitat data were collected to determine the influence of abiotic covariates on the presence of C. callainus. Cambarus callainus presence or absence and associated site covariates were modelled using logistic regression.4. Survey results recorded C. callainus at 39 sites in the Upper Levisa Fork (ULF) and Tug Fork (TF) drainages of the Big Sandy River; no C. callainus were collected in the Lower Levisa Fork (LLF). An additive effects model of physical habitat quality (Basin + Boulder presence/embeddedness) was the only model selected, supporting an association of C. callainus with slab boulders, open interstitial spaces, and moderate to no sedimentation. All sites lacking C. callainus were experiencing some degree of sedimentation. Physicochemical covariates were not supported by the data.5. Results indicated that good quality habitat was lacking in the LLF, but was present in the ULF and TF catchments, with ULF supporting the most robust populations and most suitable habitat. Effective conservation for C. callainus should focus on efforts that limit sedimentation as well as restore good quality instream habitat in the greater Big Sandy catchment.

Is there sufficient evidence for tuning fork tests in diagnosing fractures? A systematic review.

PubMed

Mugunthan, Kayalvili; Doust, Jenny; Kurz, Bodo; Glasziou, Paul

2014-08-04

To determine the diagnostic accuracy of tuning fork tests for detecting fractures. Systematic review of primary studies evaluating the diagnostic accuracy of tuning fork tests for the presence of fracture. We searched MEDLINE, CINAHL, AMED, EMBASE, Sports Discus, CAB Abstracts and Web of Science from commencement to November 2012. We manually searched the reference lists of any review papers and any identified relevant studies. Two reviewers independently reviewed the list of potentially eligible studies and rated the studies for quality using the QUADAS-2 tool. Data were extracted to form 2×2 contingency tables. The primary outcome measure was the accuracy of the test as measured by its sensitivity and specificity with 95% CIs. We included six studies (329 patients), with two types of tuning fork tests (pain induction and loss of sound transmission). The studies included patients with an age range 7-60 years. The prevalence of fracture ranged from 10% to 80%. The sensitivity of the tuning fork tests was high, ranging from 75% to 100%. The specificity of the tests was highly heterogeneous, ranging from 18% to 95%. Based on the studies in this review, tuning fork tests have some value in ruling out fractures, but are not sufficiently reliable or accurate for widespread clinical use. The small sample size of the studies and the observed heterogeneity make generalisable conclusion difficult. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Reconnaissance for radioactive deposits in Alaska, 1953

USGS Publications Warehouse

Matzko, John J.; Bates, Robert G.

1955-01-01

During the summer of 1953 the areas investigated for radioactive deposits in Alaska were on Nikolai Creek near Tyonek and on Likes Creek near Seward in south-central Alaska where carnotite-type minerals had been reported; in the headwaters of the Peace River in the eastern part of the Seward Peninsula and at Gold Bench on the South Fork of the Koyukuk River in east-central Alaska, where uranothorianite occurs in places associated with base metal sulfides and hematite; in the vicinity of Port Malmesbury in southeastern Alaska to check a reported occurrence of pitchblende; and, in the Miller House-Circle Hot Springs area of east-central Alaska where geochemical studies were made. No significant lode deposits of radioactive materials were found. However, the placer uranothorianite in the headwaters of the Peace River yet remains as an important lead to bedrock radioactive source materials in Alaska. Tundra cover prevents satisfactory radiometric reconnaissance of the area, and methods of geochemical prospecting such as soil and vegetation sampling may ultimately prove more fruitful in the search for the uranothorianite-sulfide lode source than geophysical methods.

15. View to northeast. View along centerline from below deck; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. View to northeast. View along centerline from below deck; detail of bent and floor beams. (65mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

STEEL ERECTION. View of upstream side of bridge, looking north ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

STEEL ERECTION. View of upstream side of bridge, looking north from the old suspension bridge at unjoined cantilever arms - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

16. View to southsouthwest. Detail, connection point U1 (see plans), ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. View to south-southwest. Detail, connection point U1 (see plans), from below deck. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

LOWER CHORD ERECTION. View is to northnorthwest from the old ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOWER CHORD ERECTION. View is to north-northwest from the old suspension bridge. Chord members suspended from jigger stick - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

STEEL ERECTION. View is to the north from the old ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

STEEL ERECTION. View is to the north from the old suspension bridge, looking at upstream side of new bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

4. View to westsouthwest. Oblique view of upstream side of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. View to west-southwest. Oblique view of upstream side of bridge from approximately deck level. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

117. Laurel Fork Viaduct. Elevation view of this 545 1939 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

117. Laurel Fork Viaduct. Elevation view of this 545 1939 steel girder viaduct. Example of structure with plain reinforced concrete arches. Looking northwest. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

78 FR 48901 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-12

..., 13000637 NORTH DAKOTA Grand Forks County Hariman Sanatorium, 2002 University Ave., Grand Forks, 13000633... Municipal de la Playa de Ponce, 28 Alfonso XII St., Ponce, 13000639 UTAH Salt Lake County Bennion, Howard...

Relations between precipitation and daily and monthly mean flows in gaged, unmined and valley-filled watersheds, Ballard Fork, West Virginia, 1999-2001

USGS Publications Warehouse

Messinger, Terence; Paybins, Katherine S.

2003-01-01

Large-scale surface mining using valley fills has changed hydrologic storage and processes in the Ballard Fork Watershed in West Virginia. Total unit flow for the 2-year study period (November 15, 1999?November 14, 2001) on the Unnamed Tributary (extensively mined) (11,700 cubic feet per second per square mile) was almost twice that on Spring Branch (unmined) (6,260 cubic feet per second per square mile), and about 1.75 times that on Ballard Fork (downstream, partly mined) (6,690 cubic feet per second per square mile). Unit flow from the Unnamed Tributary exceeded that from the other two streams for all flows analyzed (5?95 percent duration). Unit flow from Ballard Fork exceeded unit flow from Spring Branch about 80 percent of the time, but was about the same for high flows (less than 20 percent duration). The proportional differences among sites were greatest at low flows. Spring Branch was dry for several days in October and November 2000 and for most of October 2001, and the Unnamed Tributary had flow throughout the study period. The increase in flows from mined parts of the Ballard Fork Watershed appears to result from decreases in evapotranspiration caused by removal of trees and soil during mining. During both years, evapotranspiration from the Spring Branch Watershed greatly exceeded that from the Unnamed Tributary Watershed during May through October, when leaves were open. Evapotranspiration from the Unnamed Tributary Watershed slightly exceeded that from the Spring Branch Watershed in February and March during both years. Evapotranspiration, as a percentage of total rainfall, decreased from the first to the second, drier, year from the Unnamed Tributary Watershed (from 61 percent to 49 percent) but changed little from the Spring Branch (from 77 to 76 percent) and Ballard Fork (73 to 76 percent) Watersheds. Precipitation and flow during the study period at three nearby long-term sites, the U.S. Geological Survey stream-gaging station East Fork Twelvepole Creek near Dunlow, West Virginia, and two National Oceanic Atmospheric Administration rain gages at Madison and Dunlow, West Virginia, were less than long-term annual averages. Relations observed among the three streams in the Ballard Fork Watershed during this study may not represent those in years when annual precipitation and flow are closer to long-term averages.

Sequence-stratigraphic controls on sandstone diagenesis: An example from the Williams Fork formation, Piceance Basin, Colorado

NASA Astrophysics Data System (ADS)

Aboktef, Adel

This study documents the distribution of diagenetic alterations in Williams Fork fluvial sandstones, assess sequence stratigraphic controls on diagenetic features, and addresses diagenetic impacts on porosity. Petrographic point counts of 220 thin sections from six wells forms the database. The near absence of potassium feldspar and volcanic rock fragments in the lower Williams Fork interval and increasing plagioclase content upward represent changes in sediment provenance rather than stratigraphic variability in diagenesis. The lower Williams Fork sands are from sedimentary sources whereas middle and upper Williams Fork sands include input from magmatic arcs and basement uplifts. Compaction, early and late cementation, dissolution, and replacement by calcite or clay minerals combined to alter Williams Fork sandstones. Infiltration of clays occurred prior to any burial. Chlorite, quartz, non-ferroan calcite, compaction and dissolution features, and kaolinite formed during eo-diagenesis at <70°C. More quartz, compaction and dissolution features, plus albite, illite, mixed-layer illite/smectite, ferroan calcite, and dolomite formed in the meso-diagenetic realm (>70°C). Four of these features show spatial variability with respect to systems tracts. Infiltrated clays are concentrated in lowstand systems tracts (LST) and highstand systems tracts (HST) because accommodation space rose slow or fell during deposition of those sands, which led to prolonged sand body exposure on floodplain and ample opportunities for downward percolation of mud during flood events. Concentration of pseudomatrix (mud intraclasts) in HST and LST deposits resulted from floodplain erosion when base-level fell with decreasing accommodation space. Authigenic chlorite formed in the HST and transgressive systems tracts (TST) of the upper half of the Williams Fork Formation because volcanic clasts are abundant in that interval. Quartz overgrowths are more likely to exceed 7% in TST deposits for reasons that are unknown. High total clay content (infiltrated, grain coatings, pseudomatrix) does inhibit quartz overgrowths in all systems tracts. Williams Fork sandstones form low-permeability tight-gas reservoirs. Primary porosity was almost entirely destroyed by compaction and cementation. Reservoir rock resulted from one of two pathways. Eogenetic authigenic chlorite and/or calcite inhibited quartz cementation, minimized compaction and protected some primary porosity. Alternately, dissolution of framework grains or cements created secondary porosity. The later pathway tends to be the more dominant.

Water Quality and Biological Characteristics of the Middle Fork of the Saline River, Arkansas, 2003-06

USGS Publications Warehouse

Galloway, Joel M.; Petersen, James C.; Shelby, Erica L.; Wise, Jim A.

2008-01-01

The Middle Fork of the Saline River has many qualities that have been recognized by State and Federal agencies. The Middle Fork provides habitat for several rare aquatic species and is part of a larger stream system (the Upper Saline River) that is known for relatively high levels of species richness and relatively high numbers of species of concern. Water-quality samples were collected and streamflow was measured by the U.S. Geological Survey at three sites in the Middle Fork Basin between October 2003 and October 2006. The Arkansas Department of Environmental Quality collected discrete synoptic water-quality samples from eight sites between January 2004 and October 2006. The Arkansas Department of Environmental Quality also sampled fish (September-October 2003) and benthic macroinvertebrate communities (September 2003-December 2005) at five sites. Streamflow varied annually among the three streamflow sites from October 2003 to October 2006. The mean annual streamflow for Brushy Creek near Jessieville (MFS06) was 0.72 cubic meters per second for water years 2004-2006. The Middle Fork below Jessieville (MFS05) had a mean annual streamflow of 1.11 cubic meters per second for water years 2004-2006. The Middle Fork near Owensville (MFS02), the most downstream site, had a mean annual streamflow of 3.01 cubic meters per second. The greatest streamflows at the three sites generally occurred in the winter and spring and the least in the summer. Nutrient dynamics in the Middle Fork are controlled by activities in the basin and processes that occur in the stream. Point sources and nonpoint sources of nutrients occur in the Middle Fork Basin that could affect the water-quality. Nitrogen and phosphorus concentrations generally were greatest in Mill Creek (MFS04E) and in the Middle Fork immediately downstream from the confluence with Mill Creek (MFS04) with decreasing concentrations at sites farther downstream in Middle Fork. The site in Mill Creek is located downstream from a wastewater-treatment plant discharge and concentrations at sites farther downstream probably had lesser concentrations because of dilution effects and from algal uptake. Nutrient concentrations generally were significantly greater during high-flow conditions compared to base-flow conditions. Flow-weighted nutrient concentrations were computed for the three streamflow sites and were compared to 82 relatively undeveloped sites identified across the Nation, to the Alum Fork of the Saline River near Reform, Arkansas, and to the Illinois River south of Siloam Springs, Arkansas, a site influenced by numerous point and nonpoint sources of nutrients. Annual flow-weighted nutrient concentrations for MFS06, MFS05, and MFS02 were greater than relatively undeveloped sites, but were substantially less than the Illinois River south of Siloam Springs. Fecal indicator bacteria concentrations were slightly greater at MFS06 and MFS05 compared to concentrations at MFS02 for October 2003 to October 2006. MFS05 had the greatest E.coli concentrations and MFS06 had the greatest fecal coliform concentrations. Overall, fecal indicator bacteria concentrations were significantly greater for samples collected during high-flow conditions compared to samples collected during low-flow conditions at all three sites. Suspended-sediment concentrations did not vary significantly among MFS06, MFS05, and MFS02 for all the samples collected from October 2003 to October 2006. Suspended-sediment concentrations were significantly greater in samples collected during high-flow conditions compared to samples collected during base-flow conditions. Synoptic samples indicated varied total suspended-solids distributions from upstream to downstream in the Middle Fork between January 2004 and October 2006. Overall, total suspended-solids values were the greatest at site MFS02 and decreased at sites upstream and downstream. Turbidity measured when water-quality samples were

PLACING DIAGONALS IN CENTER PANEL. View is northnorthwest from the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PLACING DIAGONALS IN CENTER PANEL. View is north-northwest from the old suspension bridge, looking at upstream side of new bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

PLANT AND PIER #2 EXCAVATION. View is to the northeast, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PLANT AND PIER #2 EXCAVATION. View is to the northeast, looking from Humboldt County side of river toward Trinity County side - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

MOVING SHAFT FORMS FROM PIER #2 TO PIER #1. View ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

MOVING SHAFT FORMS FROM PIER #2 TO PIER #1. View is to the northeast, with shaft forms being moved by highline - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

BRIDGE BUILDER WILLIAM FLINN’S “CAMP & BRIDGE BUILDING OUTFIT”. INTERIOR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

BRIDGE BUILDER WILLIAM FLINN’S “CAMP & BRIDGE BUILDING OUTFIT”. INTERIOR VIEW SHOWING LABORERS AT MEAL TIME. - Clear Fork of Brazos River Suspension Bridge, Spanning Clear Fork of Brazos River at County Route 179, Albany, Shackelford County, TX

75 FR 51104 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-18

.... Blome Granitoid Pavement in Grand Forks Boundary Increase, Lewis Blvd between Conklin and Fenton Ave, Lewis Blvd between Fenton Ave and Seward Ave; Woodland Ave between S 4th and Grand Forks, 10000605...

8. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading crane, manufactured by Cleveland Tramrail, 2-1/2 ton capacity. - Juniata Shops, Erecting Shop & Machine Shop, East of Fourth Avenue, between Fourth & Fifth Streets, Altoona, Blair County, PA

9. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading crane, manufactured by Cleveland Tramrail, 2-1/2 ton capacity. - Juniata Shops, Erecting Shop & Machine Shop, East of Fourth Avenue, between Fourth & Fifth Streets, Altoona, Blair County, PA

Detail view of the concrete arched balustrade railing and bushhammered ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail view of the concrete arched balustrade railing and bush-hammered inset posts - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Drainage Areas of Streams at Selected Locations in Kentucky

DTIC Science & Technology

1981-01-01

23󈧸", Long 82 ° 17󈧇" 195 LICK CREEK .2 .3 .70 1 .81 03207995 Levisa Fork (Fishtrap Lake ) near Millard--Lat 37°26󈧄", Long 82 ° 24󈧾" 195 MILLARD...130 .1 209 .3 392 1,015 (Area in Kentucky) 84 .6 219 (Area outside Kentucky) 308 798 03208000 Levisa Fork (below Fishtrap Lake ) near Millard--Lat 37...547 1,417 Elkhorn Creek Basin--at Russell Fork mile 12 .3 Little Elkhorn Creek at Jenkins--Lat 37 ° 10󈧊", Long 82°38󈧊", at Elkhorn Lake outlet

Grand Forks - East Grand Forks Urban Water Resources Study. Grand Forks Flood Fight Manual

DTIC Science & Technology

1981-07-01

insurance is available for residential and commercial structures and contents at actuarial rates. As of 30 April 1980, 1258 policies were in effect...STATION #3 (WW) CLOSE SHEAR OATE ON LEW« M.VD. ON RIVER SOE OF IMKE. (S) PUCE PUMP M CATCH ■ ASM ON OTHER «DC OF MKE CLOSE SHEAR OATE ON OKE AT ISIS mVBMM...DRIVE. (S>* PUCE PUMP M CATCH « ASM ON OTHER SBC OF (NKE CLOU SHEAR OATE AT UFT STATION «S M MANHOU «WW> SWITCH OVER UFT STATION «I - CLOSE OATE

Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante

Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteinsmore » retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. We suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal.« less

Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling.

PubMed

Cobb, Andrew M; Murray, Thomas V; Warren, Derek T; Liu, Yiwen; Shanahan, Catherine M

2016-09-02

The accumulation of prelamin A is linked to disruption of cellular homeostasis, tissue degeneration and aging. Its expression is implicated in compromised genome stability and increased levels of DNA damage, but to date there is no complete explanation for how prelamin A exerts its toxic effects. As the nuclear lamina is important for DNA replication we wanted to investigate the relationship between prelamin A expression and DNA replication fork stability. In this study we report that the expression of prelamin A in U2OS cells induced both mono-ubiquitination of proliferating cell nuclear antigen (PCNA) and subsequent induction of Pol η, two hallmarks of DNA replication fork stalling. Immunofluorescence microscopy revealed that cells expressing prelamin A presented with high levels of colocalisation between PCNA and γH2AX, indicating collapse of stalled DNA replication forks into DNA double-strand breaks. Subsequent protein-protein interaction assays showed prelamin A interacted with PCNA and that its presence mitigated interactions between PCNA and the mature nuclear lamina. Thus, we propose that the cytotoxicity of prelamin A arises in part, from it actively competing against mature lamin A to bind PCNA and that this destabilises DNA replication to induce fork stalling which in turn contributes to genomic instability.

Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

DOE PAGES

Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante; ...

2015-09-08

Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteinsmore » retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. We suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal.« less

Water-quality trends for selected sampling sites in the upper Clark Fork Basin, Montana, water years 1996-2010

USGS Publications Warehouse

Sando, Steven K.; Vecchia, Aldo V.; Lorenz, David L.; Barnhart, Elliott P.

2014-01-01

A large-scale trend analysis was done on specific conductance, selected trace elements (arsenic, cadmium, copper, iron, lead, manganese, and zinc), and suspended-sediment data for 22 sites in the upper Clark Fork Basin for water years 1996–2010. Trend analysis was conducted by using two parametric methods: a time-series model (TSM) and multiple linear regression on time, streamflow, and season (MLR). Trend results for 1996–2010 indicate moderate to large decreases in flow-adjusted concentrations (FACs) and loads of copper (and other metallic elements) and suspended sediment in Silver Bow Creek upstream from Warm Springs. Deposition of metallic elements and suspended sediment within Warm Springs Ponds substantially reduces the downstream transport of those constituents. However, mobilization of copper and suspended sediment from floodplain tailings and stream banks in the Clark Fork reach from Galen to Deer Lodge is a large source of metallic elements and suspended sediment, which also affects downstream transport of those constituents. Copper and suspended-sediment loads mobilized from within this reach accounted for about 40 and 20 percent, respectively, of the loads for Clark Fork at Turah Bridge (site 20); whereas, streamflow contributed from within this reach only accounted for about 8 percent of the streamflow at Turah Bridge. Minor changes in FACs and loads of copper and suspended sediment are indicated for this reach during 1996–2010. Clark Fork reaches downstream from Deer Lodge are relatively smaller sources of metallic elements than the reach from Galen to Deer Lodge. In general, small decreases in loads and FACs of copper and suspended sediment are indicated for Clark Fork sites downstream from Deer Lodge during 1996–2010. Thus, although large decreases in FACs and loads of copper and suspended sediment are indicated for Silver Bow Creek upstream from Warm Springs, those large decreases are not translated to the more downstream reaches largely because of temporal stationarity in constituent transport relations in the Clark Fork reach from Galen to Deer Lodge. Unlike metallic elements, arsenic (a metalloid element) in streams in the upper Clark Fork Basin typically is mostly in dissolved phase, has less variability in concentrations, and has weaker direct relations with suspended-sediment concentrations and streamflow. Arsenic trend results for 1996–2010 indicate generally moderate decreases in FACs and loads in Silver Bow Creek upstream from Opportunity. In general, small temporal changes in loads and FACs of arsenic are indicated for Silver Bow Creek and Clark Fork reaches downstream from Opportunity during 1996–2010. Contribution of arsenic (from Warm Springs Ponds, the Mill-Willow bypass, and groundwater sources) in the Silver Bow Creek reach from Opportunity to Warm Springs is a relatively large source of arsenic. Arsenic loads originating from within this reach accounted for about 11 percent of the load for Clark Fork at Turah Bridge; whereas, streamflow contributed from within this reach only accounted for about 2 percent of the streamflow at Turah Bridge.

KSC-02pp0467

NASA Image and Video Library

2002-04-08

KENNEDY SPACE CENTER, FLA. -- Birds - herons or storks -- in the area of Launch Pad 39B beat a swift retreat as Space Shuttle Atlantis roars into the sky on mission STS-110. Flames from the solid rocket boosters look like inverted torches. Liftoff occurred at 4:44:19 p.m. EDT (20:41:19 GMT). Carrying the S0 Integrated Truss Structure and Mobile Transporter, STS-110 is the 13th assembly flight to the International Space Station

12. Sewage Ejector Pumps, view to the southwest. These pumps ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Sewage Ejector Pumps, view to the southwest. These pumps are connected to sewage treatment tanks. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

POURING FOOTING OF PIER #1. View is to the southwest, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

POURING FOOTING OF PIER #1. View is to the southwest, looking from Pier 2 in Trinity County toward Pier 1 in Humboldt County - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

COUNTERWEIGHT ON WEST SIDE. View is to the northwest of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

COUNTERWEIGHT ON WEST SIDE. View is to the northwest of counterweight on Humboldt County end of bridge, probably taken from the old suspension bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

15. Potential Transformer for Unit 2 and Operating Floor Front ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. Potential Transformer for Unit 2 and Operating Floor Front Corridor, view to the east-southeast. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

Detail view of substructure, view looking south at the center ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail view of substructure, view looking south at the center in-water frame bent - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

77 FR 66541 - Safety Zone; Alliance Road Bridge Demolition; Black Warrior River, Locust Fork; Birmingham, AL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-06

... 1625-AA00 Safety Zone; Alliance Road Bridge Demolition; Black Warrior River, Locust Fork; Birmingham... of the Alliance Road Bridge (Co. Rd. 61). Entry into, transiting or anchoring in this zone is.... [[Page 66542

Recent sedimentation and surface-water flow patterns on the flood plain of the North Fork Forked Deer River, Dyer County, Tennessee

USGS Publications Warehouse

Wolfe, W.J.; Diehl, T.H.

1993-01-01

Sedimentation in the 19th and 20th centuries has had a major effect on surface-water drainage conditions along a 7-mile section of the North, Fork Forked Deer River flood plain, Dyer County, Tenn. During the century prior to 1930, 5 to 12 feet of sediment were deposited over much of the flood plain, resulting in channel obstruction and widespread flooding. The estimated bankfull capacity of the natural channel before it was channelized in 19 16 was comparable to the base flow of the river during the 1980's. Ditching of the river between 191i6 and 1;9,21 was followed by reductions in sedimentation rates over parts of the flood plain. However, the effects of sedimentation have persisted. Occlusions along the natural channel of the river have divided this stream reach into a series of sloughs. These sloughs continue to fill with sediment and are surrounded by ponds that have expanded since 1941. Degradation of the North Fork Forked Deer ditch may eventually reduce ponding over much of the flood plain. Active incision of headcuts in both banks of the ditch is enhancing the drainage of widespread ponded areas. These headcuts likely will have limited effect on drainage of most tributaries. The highest recent sedimentation rates, in places more than 0.2 foot per year, are concentrated near the flood-plain margin along tributary streams. In conjunction with beaver dams and debris, ongoing sedimentation has blocked flow in several tributaries, posing a flood hazard to agricultural land near the flood-plain margin. The occluded tributaries likely will continue to overflow unless they are periodically dredged or their sediment loads are reduced.

Oak Grove Fork Habitat Improvement Project, 1988 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bettin, Scott

The Lower Oak Grove Fork of the Clackamas River is a fifth-order tributary of the Clackamas River drainage supporting depressed runs of coho and chinook salmon, and summer and winter steelhead. Habitat condition rating for the Lower Oak Grove is good, but smelt production estimates are below the average for Clackamas River tributaries. Limiting factors in the 3.8 miles of the Lower Oak Grove supporting anadromous fish include an overall lack of quality spawning and rearing habitat. Beginning in 1986. measures to improve fish habitat in the Lower Oak Grove were developed in coordination with the Oregon Department of Fishmore » and Wildlife (ODF&W) and Portland General Electric (PGE) fisheries biologists. Prior to 1986, no measures had been applied to the stream to mitigate for PGE's storage and regulation of flows in the Oak Grove Fork (Timothy Lake, Harriet Lake). Catchable rainbow trout are stocked by ODF&W two or three times a year during the trout fishing season in the lowermost portion of the Oak Grove Fork near two Forest Service campgrounds (Ripplebrook and Rainbow). The 1987 field season marked the third year of efforts to improve fish habitat of the Lower Oak Grove Fork and restore anadromous fish production. The efforts included the development of an implementation plan for habitat improvement activities in the Lower Oak Grove Fork. post-project monitoring. and maintenance of the 1986 improvement structures. No new structures were constructed or placed in 1987. Fiscal year 1988 brought a multitude of changes which delayed implementation of plans developed in 1987. The most prominent change was the withdrawal of the proposed Spotted Owl Habitat Area (SOHA) which overlapped the Oak Grove project implementation area. Another was the change in the Forest Service biologist responsible for implementation and design of this project.« less

Dietary effects of metals-contaminated invertebrates from the Coeur d'Alene River, Idaho, on cutthroat trout

USGS Publications Warehouse

Farag, A.M.; Woodward, D.F.; Brumbaugh, W.; Goldstein, J.N.; MacConnell, Elizabeth; Hogstrand, Christer; Barrows, F.T.

1999-01-01

Benthic macroinvertebrates with elevated concentrations of metals were collected from the Coeur d'Alene (CDA) River, Idaho, pasteurized, and fed to cutthroat trout Oncorhynchus clarki in the laboratory from start of feeding until 90 d posthatch. Invertebrates were collected from two sites known to contain elevated concentrations of metals: near Pinehurst in the South Fork of the CDA River and at Cataldo, approximately 5 km below the confluence of the South Fork and the North Fork. Invertebrates collected from a relatively clean site in the North Fork were used as a reference diet. We performed measurements of fish health that indicate reduced fitness of fish fed the South Fork and Cataldo diets. Effects measured were reduced feeding activity, increased number of macrophage aggregates and hyperplasia of cells in the kidney, degeneration of mucosal epithelium in the pyloric caecae, and metallothionein induction. These effects would likely reduce growth and survival of fish in the wild. Vacuolization of glial cells were also observed in fish fed the Cataldo diet. Metals in the water often exacerbated the histological effects observed. Although the invertebrates collected near Cataldo had lower concentrations of arsenic (As), cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn) than the invertebrates from the South Fork, fish fed the Cataldo diet had equally high or higher concentrations of all metals except as by day 44. The Cataldo diet also caused the most deleterious effects on survival and growth. These findings are especially important for early life stage fish, whose diet consists wholly of benthic macroinvertebrates. Therefore, fish feeding on invertebrates in the CDA River below the Bunker Hill smelting complex are at risk of reduced fitness.

Channelization and floodplain forests: Impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

USGS Publications Warehouse

Oswalt, S.N.; King, S.L.

2005-01-01

We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased overbank flooding, a rise in the groundwater table, and ponding of upstream timber. Our objectives were to: (1) determine the composition of floodplain vegetation communities along the degraded river reach, (2) to isolate relationships among these communities, geomorphic features, and environmental variables and (3) evaluate successional changes based on current stand conditions. Vegetation communities were not specifically associated with predefined geomorphic features; nevertheless, hydrologic and geomorphic processes as a result of channelization have clearly affected vegetation communities. The presence of valley plugs and continued degradation of upstream reaches and tributaries on the impacted study reach has arrested recovery of floodplain plant communities. Historically common species like Liquidambar styraciflua L. and Quercus spp. L. were not important, with importance values (IV) less than 1, and occurred in less than 20% of forested plots, while Acer rubrum L., a disturbance-tolerant species, was the most important species on the site (IV = 78.1) and occurred in 87% of forested plots. The results of this study also indicate that channelization impacts on the Middle Fork Forked Deer River are more temporally and spatially complex than previously described for other river systems. Rehabilitation of this system necessitates a long-term, landscape-scale solution that addresses watershed rehabilitation in a spatially and temporally hierarchical manner. ?? 2005 Elsevier B.V. All rights reserved.

Gold placers of the historical Fortymile River region, Alaska

USGS Publications Warehouse

Yeend, Warren E.

1996-01-01

The Fortymile River region in east-central Alaska has a long and colorful history as the site of the first major gold discovery in interior Alaska. Placer gold has been mined in the region nearly every year since its original discovery in 1886. Total gold production is approximately 500,000 troy ounces. Although many of the rich deposits have been mined, there still exist areas that contain gold. Areas of mined and unmined gold-bearing creek and terrace gravels are outlined on the accompanying geologic map. The early history of the Fortymile area centered on the small frontier settlement of Fortymile City located at the junction of the Fortymile and Yukon Rivers in Canadian territory. This was the supply and jumping-off point for prospectors who worked their way into Alaska up the Fortymile River and found gold on many of its tributaries. Hand mining, both underground and surface, using sluice boxes and (or) rockers were the earliest methods; later, hydraulicking, dredging, and draglining methods were used. More recently, bulldozers and elevated trammels have been used, as well as very portable floating suction dredges. The rich mining lore of the area is closely associated with events of the nearby world-famous Klondike District. Bedrock and placer geology and mining history of individual gold-rich creeks are herein updated. The Fortymile area, which is part of the Yukon-Tanana Upland, contains quartzite, schist, gneiss, amphibolite, marble, serpentinite, and granite overlain by basalt, sandstone, conglomerate, shale, tuff, and coal; overlying these rocks are several deposits of varying ages consisting of gold-bearing gravel and colluvium. The close spatial association of creeks containing placer gold and the gneiss, schist, amphibolite, and marble unit strongly suggests this metamorphic unit is the gold source. High terrace gravels record a time from the late Tertiary to early Pleistocene when the ancestral Fortymile River and its major tributaries, the North and South Forks, had floodplains roughly 1 to 2 miles (2-3 kilometers) wide and gradients of about 4 feet per mile (0.75 meters per kilometer). Base-level lowering during the post-early Pleistocene caused the rivers to cut into their floodplains and to develop the youthful characteristics they have today such as V-shaped canyons, narrow floodplains, and gradients of at least twice those of the old river. Colluvium marginal to creek deposits in steep-sided valleys is often gold bearing. Much of the unconsolidated gravel within the major drainages of the Fortymile River, South Fork, North Fork, and Mosquito Fork is colluvium.Heavy-mineral-concentrate samples from the gold-producing creeks and high terrace gravels contain varying amounts of magnetite (20 to 80 percent) and ilmenite (10 to 30 percent), and samples from creeks draining areas principally composed of metamorphic rocks contain abundant garnet (10 to 30 percent). Gold fineness ranges from 620 to 927, but it is difficult to attach any geologic significance to the fineness data.Most placer gold in the Fortymile River area has been recovered at, or near, the gravelbedrock contact. The lowermost 3.3 feet (1 meter) of gravel and the uppermost 1.6 feet (0.5 meter) of bedrock may contain as much as 80 to 90 percent of the gold that is ultimately recovered. Gold nuggets are rare and most of the gold recovered is in the form of flattened fragments less than .2 inches (5 millimeters) in greatest dimension. However, large gold nuggets have been found on Wade Creek; examples are ones of 25,33,56, and 70 ounces. Occasionally, large nuggets may still be found in the tailing piles along the creek. The Fortymile River and its tributaries the South Fork, Walker Fork, and Mosquito Fork, all of which at one time were the sites of bucket-line dredge operations, now are almost exclusively mined using floating suction dredges. Unmined gold-bearing gravel is present in the floodplain of the Walker Fork valley below Cherry Creek and in low (about 100 to 130 feet or 30 to 40 meters) terraces along the north side of Walker Fork and east side of Cherry Creek. Considering the locations of where most gold has been found in the South Fork valley both by the older bucket dredges and the modern suction dredges, it seems likely that the tributary drainages of Lost Chicken, Napoleon, Franklin, and Buckskin Creeks have supplied the bulk of the gold to the South Fork valley. A quarter acre (0.10 hectare), 130-foot-thick ( 40 meters) section of the high terrace gravels on the north side of Napoleon Creek was mined for placer gold and yielded values estimated to be $8.50 per cubic yard (or $6.50 per cubic meter) at $350 per troy ounce. The unmined high terrace gravels on the south side of Buckskin Creek contain gold; however, this gravel is only 3 to 6.5 feet (1 to 2 meters) thick. The search for a lode gold source in the Fortymile River region may be in vain, because substantially more gold than has been recovered from the placers can be derived by the gradual erosion of large volumes of source rocks that contain background mean gold amounts. Using Leon's mass balance equation, 5,167 metric tons of gold may exist in the placers of the Fortymile River region, less than 1 percent of the recovered amount of 15.6 tons. The largest gold resource remaining in the Fortymile River region is probably in the high terrace gravels exposed along many of the creeks and rivers. Until there is exploratory drilling or a comprehensive sampling program, the amount of gold in these gravels will remain unknown. Environmental constraints imposed by Federal and State agencies have slowed, but not stopped, placer mining in the Fortymile River area, and a significant gold price rise would result in more mining.

76 FR 13172 - Placer County Water Agency

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-10

... Water Agency Notice of Application Tendered for Filing with the Commission and Establishing Procedural... County Water Agency e. Name of Project: Middle Fork American River Project f. Location: The Middle Fork...) h. Applicant Contact: Andy Fecko, Project Manager, Placer County Water Agency, 144 Ferguson Road...