Sample records for chestnuts preliminary study

  1. Preliminary report on the segregation of resistance in chestnuts to infestation by oriental chestnut gall wasp

    Treesearch

    S Anagnostakis; Stacy Clark; Henry Mcnab

    2009-01-01

    In 1995, hybrid chestnuts were planted in North Carolina, (southern U.S.A.),where the introduced insect Oriental Chestnut Gall Wasp (Dryocosmus kuriphilus) ispresent. Of the 93 trees planted, 53 survived 12 years and were evaluated for the

  2. Ecology and pathology of European chestnut (Castanea sativa) in the deciduous forests of the Caucasus Mountains in southern Russia

    USGS Publications Warehouse

    Pridnya, M.V.; Cherpakov, V.V.; Paillet, Frederick L.

    1996-01-01

    Chestnut-dominated forests of the Caucasus Mountain area of Russia are very similar to former chestnut-dominated forests in eastern North America. The distribution, pathology, and reproductive status of European chestnut (Castanea sativa) in the Caucasus are described and compared to that of American chestnut (C. dentata). Chestnut forests are distributed continuously along the southern slope of the Caucasus mountains near the Black Sea, and are found in isolated populations on the north side of the Caucasus, at elevations ranging from 200 to 1300 meters. Chestnut blight was apparently introduced into the region after 1880 and continues to destroy chestnut forests today. Chestnut in the Caucasus is also infected by several other fungal and bacterial parasites and the joint infection of blight and bacteria may be especially dangerous for chestnut trees. Chestnut-dominated forests comprise only a few percent of total forest cover in the Caucasus Biosphere Preserve, and usually occur in mountain valleys or coves with deep brown soil. The age structure and reproductive status of chestnut in the Caucasus was investigated on six study plots in the Caucasus Biosphere Forest Preserve near the upper altitudinal limit of chestnut. Although chestnut is at least 70 percent of the overstory on these sites, there are very few trees less than 50 years old, and very few recent seedlings on any of the plots. Most large chestnut trees appear to have originated as basal spouts from previously established stems. Although chestnut seed production appears adequate, we suspect that competition with shrubs and other tree seedlings, and predation by herbivores and rodents, now prevent the establishment and survival of chestnut seedlings in the Biosphere Preserve.

  3. Impact of silvicultural treatment on chestnut seedling growth and survival

    Treesearch

    C.C. Pinchot; S.E. Schlarbaum; S.L. Clark; C.J. Schweitzer; A.M. Saxton; F. V. Hebard

    2014-01-01

    Putatively blight-resistant advanced backcross chestnut seedlings will soon be available for outplanting on a regional scale. Few studies have examined the importance of silvicultural treatment or seedling quality to chestnut reintroduction in the U.S. This paper examines results from a silvicultural study of high-quality chestnut seedlings on the Cumberland Plateau of...

  4. The importance of site quality to backcross chestnut establishment success

    Treesearch

    C.C. Pinchot; A.A. Royo; M.P. Peters; S.E. Schlarbaum; S.L. Anagnostakis

    2017-01-01

    Short-term studies show that American chestnut (Castanea dentata) grows faster on mesic compared to xeric sites. Long-term impacts of site quality and corresponding moisture and nutrient availability on backcross chestnut establishment success and resistance to the chestnut blight fungus, Cryphonectria parasitica, however, have...

  5. Plumage coloration and reproductive success in male chestnut-sided warblers

    Treesearch

    David I. King; Richard M. DeGraaf; Curtice R. Griffin

    2001-01-01

    We studied Chestnut-sided Warblers (Dendroica pensylvanica) to determine whether there exists any relationship between plumage coloration and reproductive success in this species. We observed that males with more extensive chestnut breast coloration initiated nests significantly earlier than males with less chestnut, and had marginally larger...

  6. Spray-dried chestnut extract containing Lactobacillus rhamnosus cells as novel ingredient for a probiotic chestnut mousse.

    PubMed

    Romano, A; Blaiotta, G; Di Cerbo, A; Coppola, R; Masi, P; Aponte, M

    2014-06-01

    Consumers' demand for innovative probiotic products has recently increased. In previous studies, chestnuts were evaluated as substrate for the growth of lactobacilli and chestnut extract was found to enhance acid tolerance of probiotic strains. The main objectives of this study were to evaluate the suitability of chestnut extract as carrier for spray drying of two probiotic Lactobacillus rhamnosus strains and to develop a probiotic food chestnut based. The optimal settings for the spray-drying processes were defined and the loads of undamaged cells in the dried powders were quantified. Spray-dried cultures were incorporated into an anhydrous basis for chestnut mousse developed ad hoc. In this form, viable cells remained stable over 10(8) CFU g(-1) during a 3 months long storage at 15°C. Sensorial analysis did not highlighted significant differences (P < 0·05) in preference between probiotic-supplemented and control mousses. Results suggest that chestnut mousse, a food product naturally rich in antioxidant compounds, may represent an excellent carrier for probiotics delivering. To authors' knowledge, this is the first information on the survival of lactobacilli in an anhydrous basis for dessert. © 2014 The Society for Applied Microbiology.

  7. Detection of Oil Chestnuts Infected by Blue Mold Using Near-Infrared Hyperspectral Imaging Combined with Artificial Neural Networks.

    PubMed

    Feng, Lei; Zhu, Susu; Lin, Fucheng; Su, Zhenzhu; Yuan, Kangpei; Zhao, Yiying; He, Yong; Zhang, Chu

    2018-06-15

    Mildew damage is a major reason for chestnut poor quality and yield loss. In this study, a near-infrared hyperspectral imaging system in the 874⁻1734 nm spectral range was applied to detect the mildew damage to chestnuts caused by blue mold. Principal component analysis (PCA) scored images were firstly employed to qualitatively and intuitively distinguish moldy chestnuts from healthy chestnuts. Spectral data were extracted from the hyperspectral images. A successive projections algorithm (SPA) was used to select 12 optimal wavelengths. Artificial neural networks, including back propagation neural network (BPNN), evolutionary neural network (ENN), extreme learning machine (ELM), general regression neural network (GRNN) and radial basis neural network (RBNN) were used to build models using the full spectra and optimal wavelengths to distinguish moldy chestnuts. BPNN and ENN models using full spectra and optimal wavelengths obtained satisfactory performances, with classification accuracies all surpassing 99%. The results indicate the potential for the rapid and non-destructive detection of moldy chestnuts by hyperspectral imaging, which would help to develop online detection system for healthy and blue mold infected chestnuts.

  8. Effects of temporal dynamics, nut weight and nut size on growth of American chestnut, Chinese chestnut and backcross generations in a commercial nursery

    Treesearch

    Cornelia Pinchot; Stacy Clark; Scott Schlarbaum; Arnold Saxton; Shi-Jean Sung; Frederick. Hebard

    2015-01-01

    Blight-resistant American chestnut (Castanea dentata) may soon be commercially available, but few studies have tested methods to produce high quality seedlings that will be competitive after planting. This study evaluated the performance of one American, one Chinese (C. mollissima), one second-generation backcross (BC3...

  9. The American chestnut and fire: 6-year research results

    Treesearch

    Stacy L. Clark; Callie J. Schweitzer; Mike R. Saunders; Ethan P. Belair; Scott J. Torreano; Scott E. Schlarbaum

    2014-01-01

    American chestnut [Castanea dentata Marsh. (Borkh.)] is an iconic species with important ecological and utilitarian values, but was decimated by the mid-20th century by exotic fungal species fromAsia. Successful restoration will require sustainable silvicultural methods to maximize survival and afford chestnut a competitive advantage over natural vegetation. The study...

  10. Do chestnut, northern red, and white oak germinant seedlings respond similarly to light treatments? Growth and biomass

    Treesearch

    Joanne Rebbeck; Kurt Gottschalk; Amy Scherzer

    2011-01-01

    Northern red oak (Quercus rubra L.) seedling growth has been extensively studied. White oak (Quercus alba L.) and chestnut oak (Quercus prinus L.), however, are far less investigated despite their importance among upland oak species in eastern North American forests. We characterized white and chestnut oak...

  11. Markers linked to vegetative incompatibility (vic) genes and a region of high heterogeneity and reduced recombination near the mating type locus (MAT) in Cryphonectria parasitica

    Treesearch

    Thomas L. Kubisiak; Michael g. Milgroom

    2006-01-01

    To find markers linked to vegetative incompatibility (vic) genes in the chestnut blight fungus, Cryphonectria parasitica, we constructed a preliminary linkage map. In general, this map is characterized by low levels of polymorphism, as evident from the more than 24 linkage groups observed, compared to seven expected from electrophoretic karyotyping....

  12. Castanea sativa by-products: a review on added value and sustainable application.

    PubMed

    Braga, Nair; Rodrigues, Francisca; Oliveira, M Beatriz P P

    2015-01-01

    Castanea sativa Mill. is a species of the family Fagaceae abundant in south Europe and Asia. The fruits (chestnut) are an added value resource in producing countries. Chestnut economic value is increasing not only for nutritional qualities but also for the beneficial health effects related with its consumption. During chestnut processing, a large amount of waste material is generated namely inner shell, outer shell and leaves. Studies on chestnut by-products revealed a good profile of bioactive compounds with antioxidant, anticarcinogenic and cardioprotective properties. These agro-industrial wastes, after valorisation, can be used by other industries, such as pharmaceutical, food or cosmetics, generating more profits, reducing pollution costs and improving social, economic and environmental sustainability. The purpose of this review is to provide knowledge about the type of chestnut by-products produced, the studies concerning its chemical composition and biological activity, and also to discuss other possible applications of these materials.

  13. Effect of whey protein isolate-pullulan edible coatings on the quality and shelf life of freshly roasted and freeze-dried Chinese chestnut.

    PubMed

    Gounga, M E; Xu, S-Y; Wang, Z; Yang, W G

    2008-05-01

    Harvested chestnut is characterized by a short shelf life, exposing many Chinese producers to a storage problem as product losses are very high. The objective of this study was to develop a suitable technology to extend the shelf life of harvested chestnut fruits for commercial use. The effect of whey protein isolate-pullulan (WPI-Pul) coating on fresh-roasted chestnuts (FRC) and roasted freeze-dried chestnut (RFDC) quality and shelf life was studied under 2 different storage temperature (4 and 20 degrees C) conditions. Coatings were formed directly onto the surface of the fruits by dipping them into a film solution. SEM micrographs showed homogeneous WPI-Pul to cover the whole surface of chestnut with good adherence and perfect integrity. Moisture loss or gain, fruit quality, and shelf life were evaluated by weight loss or gain, surface color development, and visible decay during the storage period of 15 to 120 d at 4 and 20 degrees C, respectively. WPI-Pul coating had a low, yet significant effect on reducing moisture loss and decay incidence of FRC, hence delaying changes in their external color. The results were satisfactory when the coating was done with freeze-drying at low temperature storage, thus improving the quality and increasing the shelf life. This provides an alternative strategy to minimize the significant losses in harvested chestnut.

  14. [Bioactive saponins and glycosides. XIII. Horse chestnut. (3): Quantitative analysis of escins Ia, Ib, IIa, and IIb by means of high performance liquid chromatography].

    PubMed

    Yoshikawa, M; Murakami, T; Otuki, K; Yamahara, J; Matsuda, H

    1999-01-01

    As a part of our studies on the characterization of bioactive saponin constituents of horse chestnut trees, a quantitative method using high performance liquid chromatography (HPLC) has been developed for four principle saponin constituents, such as escins Ia, Ib, IIa, and IIb, isolated from the seeds of European horse chestnut trees (Aesculus hippocastanum L., Hippocastanaceae). As an application of this HPLC method, we examined the contents and compositions of these escins in the seeds of Japanese horse chestnut trees (A. turbinata BLUME) and in several commercial materials named as "beta-escin". Additionally, the distribution of escins in the Japanese horse chestnut trees was examined, and escins were found to be contained only in the seeds.

  15. Outlook for blight-resistant American chestnut trees

    Treesearch

    Paul H. Sisco

    2009-01-01

    Culminating 20 years of breeding efforts, in spring 2008, The American Chestnut Foundation (TACF) delivered its first 500 chestnuts to the USDA Forest Service for testing on National Forest lands. The expectation is that these seedlings will be more resistant to chestnut blight (Cryphonectria parasitica) than are pure American chestnut trees (

  16. The Silvics of Castanea dentata (Marsh.) Borkh., American chestnut, Fagaceae (Beech Family)

    Treesearch

    G. Geoff Wang; Benjamin O. Knapp; Stacy L. Clark; Bryan T. Mudder

    2013-01-01

    This report describes how the American chestnut (Castanea dentata) was ecologically extirpated due to an exotic pathogen, the chestnut blight (Cryphonectria parasitica), and describes current restoration efforts. The habitat, life history, special uses, and genetics of the American chestnut are detailed. The American chestnut was...

  17. Winter Iinjury of American chestnut seedlings grown in a common garden at the species' northern range limit

    Treesearch

    Paul G. Schaberg; Thomas M. Saielli; Gary J. Hawley; Joshua M. Halman; Kendra M. Gurney

    2013-01-01

    Hybridization of American chestnut (Castanea dentata) with Chinese chestnut (C. mollissima), followed by backcrossing to American chestnut, is conducted to increase the resistance of resulting stock to chestnut blight, caused by the fungal pathogen Cryphonectria parasitica (Murr.) Barr. Backcross breeding is...

  18. Twolined Chestnut Borer

    Treesearch

    Robert A. Haack; Robert E. Acciavatti

    1992-01-01

    The twolined chestnut borer, Agrilus bilineatus (Weber), belongs to the beetle family Buprestidae. The word "chestnut" refers to the beetle's past status as a principal pest of American chestnut, Castanea dentata. The twolined chestnut borer is found from the Maritime Provinces of Canada, west to the Rocky Mountains, and south to Florida and Texas.

  19. Physico-chemical, rheological and antioxidant properties of sweet chestnut (Castanea sativa Mill.) as affected by pan and microwave roasting.

    PubMed

    Wani, Idrees Ahmed; Hamid, Humaira; Hamdani, Afshan Mumtaz; Gani, Adil; Ashwar, Bilal Ahmad

    2017-07-01

    Sweet chestnut ( Castanea sativa Mill. ) belongs to the family Fagaceae and sub family Castaneoideae. Bioactive components such as tannins are present in sweet chestnut in high proportion giving astringent bitter taste and reducing their palatability. Roasting reduces the anti-nutritional factors in chestnut. This study was conducted to compare the effects of pan and microwave roasting on physicochemical, functional, rheological and antioxidant properties of sweet chestnut. Antioxidant activity was determined using DPPH inhibition activity, reducing power, and total phenolic content. Structural analysis was carried out using FT-IR analysis. Protein, fat, and ash contents displayed insignificant ( P  > 0.05) variations. " L " value decreased from 90.66 to 81.43, whereas, " a " and " b " values increased from 0.02 to 0.90 and 11.99 to 20.5, respectively, upon roasting. Significant ( P  < 0.05) increase in water absorption capacity (1.32-3.39 g/g), oil absorption capacity (1.22-1.63 g/g), and antioxidant properties was observed following roasting. Flour obtained from roasted chestnuts exhibited a significant decrease in light transmittance, foaming, and pasting properties. Higher gelatinization temperatures and lower enthalpies were reported in microwave and pan roasted chestnut flours. Roasting also reduced the viscoelastic behavior of native sweet chestnut and changed the transmittance of identical functional groups as revealed by FT-IR analysis.

  20. Genetics and silvicultural treatments influence the growth and shoot winter injury of American chestnut in Vermont

    Treesearch

    Thomas M. Saielli; Paul G. Schaberg; Gary J. Hawley; Joshua M. Halman; Kendra M. Gurney

    2014-01-01

    The backcross breeding of American chestnut (Castanea dentata [Marsh.] Borkh.) with Chinese chestnut (Castanea mollissima Blume) may provide an effective method to increase resistance against chestnut blight and help restore American chestnut throughout its historic range. However, the comparative adaptation (e.g., cold hardiness...

  1. Nut cold hardiness as a factor influencing the restoration of American chestnut in northern latitudes and high elevations

    Treesearch

    Thomas M. Saielli; Paul G. Schaberg; Gary J. Hawley; Joshua M. Halman; Kendra M. Gurney

    2012-01-01

    American chestnut (Castanea dentata (Marsh.) Borkh.) was functionally removed as a forest tree by chestnut blight (caused by the fungal pathogen Cryphonectria parasitica (Murr.) Barr). Hybrid-backcross breeding between blight-resistant Chinese chestnut (Castanea mollissima Blume) and American chestnut is used to...

  2. Resistance of chestnut trees to Asia chestnut gall wasp

    Treesearch

    S. Anagnostakis; S.L. Clark; H. McNab

    2011-01-01

    Asian chestnut gall wasp (Dryocosmus kuriphihus) was introduced into Georgia (USA) in 1975 and has been spreading north throughout the range of American chestnut (Castanea dentate). This pest is now present throughout most of Tennessee. In 2003, it was found near Cleveland, Ohio and has been spreading south from there. In 1995, hybrid chestnuts with C. dentate female...

  3. The first research plantings of third-generation, third-backcross American chestnut (Castanea dentata) in the southeastern United States

    Treesearch

    Stacy Clark; S.E. Schlarbaum; F,V Saxton

    2014-01-01

    Production of American chestnut (Castanea dentata) resistant to the chestnut blight fungus (Cryphonectria parasitica) is being conducted currently through traditional breeding and genetic transformation. Sufficient material for field testing is currently available from The American Chestnut Foundation’s backcross breeding program. We planted approximately 4500 chestnut...

  4. Making history: Field testing of blight-resistant American chestnut (Castanea dentata) in the Southern Region

    Treesearch

    Stacy L. Clark; Scott E. Schlarbaum; A.M. Saxton; Fred V. Hebard

    2011-01-01

    The American chestnut (Castanea dentata Marsh. Borkh.) was decimated by an exotic fungus (chestnut blight [Cryphonectria parasitica Murr. Bar]) in the early part of the 20th century. The American Chestnut Foundation (TACF) uses a back-cross breeding program to produce a tree that is predicted to be American chestnut in character...

  5. Assessing potential changes of chestnut productivity in Europe under future climate conditions

    NASA Astrophysics Data System (ADS)

    Calheiros, T.; Pereira, M. G.; Pinto, J. G.; Caramelo, L.; Gomes-Laranjo, J.; Dacamara, C. C.

    2012-04-01

    The European chestnut is cultivated for its nuts and wood. Several studies point to the dependency of chestnut productivity on specific soil and climate characteristics. For instance, this species dislikes chalky and poorly drained soils, appreciates sedimentary, siliceous and acidic to neutral soils. Chestnut trees also seems to appreciate annual mean values of sunlight spanning between 2400 and 2600 h, rainfall ranging between 600 and 1500 mm, mean annual temperature between 9 and 13°C, 27°C being the mean of the maximum temperature (Heiniger and Conedera, 1992; Gomes-Laranjo et al.,2008). The amount of heat between May and October must range between 1800°D and 2400°D (Dinis et al., 2011) . In Poland, the growing season is defined as the period of time when the mean 24-h temperature is greater than 5°C (Wilczynski and Podalski, 2007). In Portugal, maximum photosynthetic activity occurs at 24-28°C for adult trees, but exhibits more than 50% of termoinhibition when the air temperature is above 32°C, which is frequent during summer (Gomes- Laranjo et al., 2006, 2008). Recently Pereira et al (2011) identified a set of meteorological variables/parameters with high impact on chestnut productivity. The main purpose of this work is to assess the potential impacts of future climate change on chestnut productivity in Portugal as well as on European chestnut orchards. First, observed data from the European Climate assessment (ECA) and simulations with the Regional Circulation Model (RCM) COSMO-CLM for recent climate conditions are used to assess the ability of the RCM to model the actual meteorological conditions. Then, ensemble projections from the ECHAM5/COSMO-CLM model chain for two climate scenarios (A1B and B1) are used to estimate the values of relevant meteorological variables and parameters und future climate conditions. Simulated values are then compared with those obtained for present climate. Results point to changes in the spatial and temporal distribution of meteorological variables and parameters. In particular, more severe conditions during spring and summer are expected, especially in the Mediterranean area, with less precipitation and higher temperatures. All these changes will have impacts on chestnut fruits and wood in Europe. Dinis, L-T. J., Ferreira-Cardoso, J., Peixoto, F., Costa, R. e Gomes-Laranjo, J., 2011: Study of morphological and chemical diversity in chestnut trees (var. 'Judia') as a function of temperature sum. Cyta- Journal of food, 9(3): 192-199 Gomes-Laranjo et al., 2008: Differences in photosynthetic apparatus of leaves from different sides of chestnut canopy, Photosynthetica, 46, 63-72. Heiniger,U. And Conedera, M., 1992: Chestnut forests and chestnut cultivation in Switzerland. Proceedings of the International Chestnut Conference, West Virginia University, Morgantown, 10-14 July 1992, 175-178. Pereira, M.G., Caramelo, L., Gouveia, C., Gomes-Laranjo, J., Magalhães, M., 2011: Assessment of weather-related risk on chestnut productivity. Nat. Hazards Earth Syst. Sci., 11, 1-12, doi:10.5194/nhess-11-12-011. Wilczynski, S. And Podlaski, R, 2007: The effect of climate on radial growth of horse chestnut (Aesculus hippocastanum L.) in the Swietokrzki National Park in Central Poland, J.For.Res., 12, 24-23.

  6. The content of phenolic compounds in leaf tissues of white (Aesculus hippocastanum L.) and red horse chestnut (Aesculus carea H.) colonized by the horse chestnut leaf miner (Cameraria ohridella Deschka & Dimić).

    PubMed

    Oszmiański, Jan; Kalisz, Stanisław; Aneta, Wojdyło

    2014-09-15

    Normally, plant phenolics are secondary metabolites involved in the defense mechanisms of plants against fungal pathogens. Therefore, in this study we attempted to quantify and characterize phenolic compounds in leaves of white and red horse chestnut with leaf miner larvae before and after Cameraria ohridella attack. A total of 17 phenolic compounds belonging to the hydroxycinnamic acid, flavan-3-ols and flavonol groups were identified and quantified in white and red horse chestnut leaf extracts. Significantly decreased concentrations of some phenolic compounds, especially of flavan-3-ols, were observed in infected leaves compared to the non-infected ones. Additionally, a higher content of polyphenolic compounds especially (-)-epicatechin and procyanidins in leaves of red-flowering than in white-flowering horse chestnut may explain their greater resistance to C. ohridella insects.

  7. Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control.

    PubMed

    Rigling, Daniel; Prospero, Simone

    2018-01-01

    Chestnut blight, caused by Cryphonectria parasitica, is a devastating disease infecting American and European chestnut trees. The pathogen is native to East Asia and was spread to other continents via infected chestnut plants. This review summarizes the current state of research on this pathogen with a special emphasis on its interaction with a hyperparasitic mycovirus that acts as a biological control agent of chestnut blight. Cryphonectria parasitica (Murr.) Barr. is a Sordariomycete (ascomycete) fungus in the family Cryphonectriaceae (Order Diaporthales). Closely related species that can also be found on chestnut include Cryphonectria radicalis, Cryphonectria naterciae and Cryphonectria japonica. Major hosts are species in the genus Castanea (Family Fagaceae), particularly the American chestnut (C. dentata), the European chestnut (C. sativa), the Chinese chestnut (C. mollissima) and the Japanese chestnut (C. crenata). Minor incidental hosts include oaks (Quercus spp.), maples (Acer spp.), European hornbeam (Carpinus betulus) and American chinkapin (Castanea pumila). Cryphonectria parasitica causes perennial necrotic lesions (so-called cankers) on the bark of stems and branches of susceptible host trees, eventually leading to wilting of the plant part distal to the infection. Chestnut blight cankers are characterized by the presence of mycelial fans and fruiting bodies of the pathogen. Below the canker the tree may react by producing epicormic shoots. Non-lethal, superficial or callusing cankers on susceptible host trees are usually associated with mycovirus-induced hypovirulence. After the introduction of C. parasitica into a new area, eradication efforts by cutting and burning the infected plants/trees have mostly failed. In Europe, the mycovirus Cryphonectria hypovirus 1 (CHV-1) acts as a successful biological control agent of chestnut blight by causing so-called hypovirulence. CHV-1 infects C. parasitica and reduces its parasitic growth and sporulation capacity. Individual cankers can be therapeutically treated with hypovirus-infected C. parasitica strains. The hypovirus may subsequently spread to untreated cankers and become established in the C. parasitica population. Hypovirulence is present in many chestnut-growing regions of Europe, either resulting naturally or after biological control treatments. In North America, disease management of chestnut blight is mainly focused on breeding with the goal to backcross the Chinese chestnut's blight resistance into the American chestnut genome. © 2017 BSPP AND JOHN WILEY & SONS LTD.

  8. Influence of xanthan, guar, CMC and gum acacia on functional properties of water chestnut (Trapa bispinosa) starch.

    PubMed

    Lutfi, Zubala; Nawab, Anjum; Alam, Feroz; Hasnain, Abid; Haider, Syed Zia

    2017-10-01

    This study was performed to determine the effect of xanthan, guar, CMC and gum acacia on functional and pasting properties of starch isolated from water chestnut (Trapa bispinosa). Morphological properties of water chestnut starch with CMC were studied by scanning electron microscopy (SEM). The addition of hydrocolloids significantly enhanced the solubility of water chestnut starch (WCS) while reduced swelling power and freeze-thaw stability. The hydrophilic tendency of WCS was increased by xanthan gum; however, with addition of gum acacia it decreased significantly. Starch was modified with guar and gum acacia exhibited highest% syneresis. Guar gum was found to be effective in increasing the clarity of water chestnut starch paste. The addition of CMC significantly reduced the pasting temperature of WCS indicating ease of gelatinization. The setback was accelerated in the presence of xanthan gum but gum acacia delayed this effect during the cooling of the starch paste. Only xanthan gum was found to be effective in increasing breakdown showing good paste stability of WCS. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Three American tragedies: chestnut blight, butternut canker, and Dutch elm disease

    Treesearch

    Scott E. Schlarbaum; Frederick Hebard; Pauline C. Spaine; Joseph C. Kamalay

    1998-01-01

    Three North American tree species, American chestnut (Castanea dentata), butternut (Juglans cinerea), and American elm (Ulmus americana), have been devastated by exotic fungal diseases over the last century. American chestnut was eliminated from eastern forests as a dominant species by chestnut blight (...

  10. Substantial genome synteny preservation among woody angiosperm species: comparative genomics of Chinese chestnut (Castanea mollissima) and plant reference genomes.

    PubMed

    Staton, Margaret; Zhebentyayeva, Tetyana; Olukolu, Bode; Fang, Guang Chen; Nelson, Dana; Carlson, John E; Abbott, Albert G

    2015-10-05

    Chinese chestnut (Castanea mollissima) has emerged as a model species for the Fagaceae family with extensive genomic resources including a physical map, a dense genetic map and quantitative trait loci (QTLs) for chestnut blight resistance. These resources enable comparative genomics analyses relative to model plants. We assessed the degree of conservation between the chestnut genome and other well annotated and assembled plant genomic sequences, focusing on the QTL regions of most interest to the chestnut breeding community. The integrated physical and genetic map of Chinese chestnut has been improved to now include 858 shared sequence-based markers. The utility of the integrated map has also been improved through the addition of 42,970 BAC (bacterial artificial chromosome) end sequences spanning over 26 million bases of the estimated 800 Mb chestnut genome. Synteny between chestnut and ten model plant species was conducted on a macro-syntenic scale using sequences from both individual probes and BAC end sequences across the chestnut physical map. Blocks of synteny with chestnut were found in all ten reference species, with the percent of the chestnut physical map that could be aligned ranging from 10 to 39 %. The integrated genetic and physical map was utilized to identify BACs that spanned the three previously identified QTL regions conferring blight resistance. The clones were pooled and sequenced, yielding 396 sequence scaffolds covering 13.9 Mbp. Comparative genomic analysis on a microsytenic scale, using the QTL-associated genomic sequence, identified synteny from chestnut to other plant genomes ranging from 5.4 to 12.9 % of the genome sequences aligning. On both the macro- and micro-synteny levels, the peach, grape and poplar genomes were found to be the most structurally conserved with chestnut. Interestingly, these results did not strictly follow the expectation that decreased phylogenetic distance would correspond to increased levels of genome preservation, but rather suggest the additional influence of life-history traits on preservation of synteny. The regions of synteny that were detected provide an important tool for defining and cataloging genes in the QTL regions for advancing chestnut blight resistance research.

  11. Status and future of breeding disease-resistant American chestnut

    Treesearch

    J. Westbrook; F.V. Hebard; S.F. Fitzsimmons; J. Donahue

    2017-01-01

    The American Chestnut Foundation (TACF) has worked since 1983 to introduce genetic resistance to the chestnut blight fungus (Cryphonectria parasitica) into an American chestnut (Castanea dentata) population. As part of a broader goal for species restoration, TACF seeks to instill within that population sufficient diversity so as to enable the...

  12. The implications of American chestnut reintroduction on landscape dynamics and carbon storage

    Treesearch

    Eric J. Gustafson; Arjan de Bruijn; Nathanael Lichti; Douglass F. Jacobs; Brian R. Sturtevant; Jane Foster; Brian R. Miranda; Harmony J. Dalgleish

    2017-01-01

    In the eastern United States, American chestnut (Castanea dentata) was historically a major component of forest communities, but was functionally extirpated in the early 20th century by an introduced pathogen, chestnut blight (Cryphonectria parasitica). Because chestnut is fast-growing, long-lived, and resistant to decay,...

  13. Ectomycorrhizal inoculum potential of northeastern U.S. forest soils for American chestnut: results from field and laboratory bioassays

    EPA Science Inventory

    American chestnut (Castanea dentata) was once a dominant overstory tree in the eastern United States but was decimated by chestnut blight (Cryphonectria parasitica). Blight resistant chestnut is being developed as part of a concerted restoration effort to bring this heritage tree...

  14. Current status of chestnut in eastern US forests

    Treesearch

    William H. McWiliams; Tonya W. Lister; Elizabeth B. LaPoint; Anita K. Rose; John S. Vissage

    2006-01-01

    The USDA Forest Service, Forest Inventory and Analysis (FIA) program provides the opportunity to assess the current distribution of American chestnut (Castanea dentata (Marsh.) Borkh) and prospective trends. Assessing chestnut using the FIA data was challenging because of the coarse nature of the FIA sample and chestnut's rarity in natural...

  15. American chestnut as an allelopath in the southern Appalachians

    Treesearch

    D.B. Vandermast; David H. van Lear; B.D. Clinton

    2002-01-01

    Prior to the chestnut blight (Crypkonectria parasitica), American chestnut (Castanea dentata (Marsh.) Borkh.) was the most common overstory tree in eastern deciduous forests. Chestnut's dominance has often been attributed to its resistance to fire and subsequent propensity to sprout vigorously and grow rapidly. Its role as an allelopath has...

  16. Can our chestnut survive another invasion?

    Treesearch

    Lynne K. Rieske; W. Rodney Cooper

    2011-01-01

    Plant breeders and land managers have been actively pursuing development of an American chestnut with desirable silvicultural characteristics that demonstrates resistance to the chestnut blight fungus. As progress towards development of a blight-resistant chestnut continues, questions arise as to how these plants will interact with pre-existing stresses. The Asian...

  17. Assessment of the chestnut production weather dependence

    NASA Astrophysics Data System (ADS)

    Pereira, Mário; Caramelo, Liliana; Gouveia, Célia; Gomes-Laranjo, José

    2010-05-01

    The vegetative cycle of chestnut trees is highly dependent on weather. Photosynthesis and pollen germination are mainly conditioned by the air temperature while heavy precipitation and strong wind have significant impacts during the flushing phase period (Gomes-Laranjo et al., 2005, 2006). In Portugal, chestnut tree orchads are located in mountainous areas of the Northeast region of Trás-os-Montes, between 600 and 1000 m of altitude. Topography controls the atmospheric environment and assures adequate conditions for the chestnut production. In the above mentioned context, remote sensing plays an important role because of its ability to monitor and characterise vegetation dynamics. A number of studies, based on remote sensing, have been conducted in Europe to analyse the year-to-year variations in European vegetation greenness as a function of precipitation and temperature (Gouveia et al., 2008). A previous study focusing on the relationship between meteorological variables and chestnut productivity provides indication that simulation models may benefit from the incorporation of such kind of relationships. The aim of the present work is to provide a detailed description of recent developments, in particular of the added value that may be brought by using satellite data. We have relied on regional fields of the Normalized Difference Vegetation Index (NDVI) dataset, at 8-km resolution, provided by the Global Inventory Monitoring and Modelling System (GIMMS) group. The data are derived from the Advanced Very High Resolution Radiometers (AVHRR), and cover the period from 1982 to 2006. Additionally we have used the chestnut productivity dataset, which includes the annual values of chestnut production and area of production provided by INE, the National Institute of Statistics of Portugal and the meteorological dataset which includes values of several variables from different providers (Meteorod, NCEP/NCAR, ECA&D and national Meteorological Institute). Results show that satellite and meteorological data are complementary in what respects to the evaluation of the spatial and temporal evolution of the chestnut production. The satellite data proves to be very useful to monitor the spatial and temporal evolution of the vegetation state in the locations of the chestnut orchads and when tested as potential predictors by means of correlation and regression analysis. Gomes-Laranjo, J., Coutinho, J.P., Ferreira-Cardoso, J., Pimentel-Pereira, M., Ramos, C., Torres-Pereira, J.(2005) "Assessment to a new concept of chestnut orchard management in vegetative wall.". Acta Hort. 693: 707-712. Gomes-Laranjo, J.C.E., Peixoto, F., Wong Fong Sang, H.W., Torres-Pereira, J.M.G.(2006) "Study of the temperature effect in three chestnut (Castanea sativa Mill.) cultivars' behavior". J. Plant Physiol. 163: 945-955. Gouveia C., Trigo R.M., DaCamara C.C., Libonati R., Pereira J.M.C., 2008b. The North Atlantic Oscillation and European vegetation dynamics. International Journal of Climatology, vol. 28, issue 14, pp. 1835-1847, DOI: 10.1002/joc.1682.

  18. Blight-resistant American chestnut trees: selection of progeny from a breeding program

    Treesearch

    Shiv Hiremath; Kirsten Lehtoma; Fred Hebard

    2007-01-01

    Introduction of the fungus Cryphonectria parasitica into North America in early 1900s resulted in the demise of the American chestnut, which was once the most dominant forest tree in the eastern United States. While the American chestnut (Castanea dentate) is susceptible, its counterpart from Asia, the Chinese chestnut, is...

  19. Establishment of American chestnuts (Castanea dentata) bred for blight (Cryphonectria parasitica ) resistance: influence of breeding and nursery grading

    Treesearch

    Stacy L. Clark; Scott E. Schlarbaum; Arnold M. Saxton; Frederick V. Hebard

    2016-01-01

    European and American chestnut species (Castanea) have been decimated by exotic species, most notably chestnut blight (Cryphonectria parasitica), since the early nineteenth century. Backcross breeding programs that transfer blight disease resistance from Chinese chestnut (C. mollissima) into American...

  20. Soil compaction and chestnut ink disease

    Treesearch

    T.F. Fonseca; C.G. Abreu; B.R. Parresol

    2004-01-01

    Chestnut ink disease caused by the oomycete Phytophthora cinnamomi, a soil-borne pathogen of world-wide distribution, accounts for the majority of disease problems on chestnuts in Portugal, limiting yield in a large number of stands and impeding establishment of trees in new areas. A survey was carried out in 32 chestnut stands in the Padrela...

  1. Cooperative test plots produce some promising Chinese and hybrid chestnut trees

    Treesearch

    Jesse D. Diller; Russell B. Clapper; Richard A. Jaynes

    1964-01-01

    In attempts to find a chestnut tree that is resistant to the blight fungus Endothia parasitica, Asiatic chestnuts have been imported and grown in this country, and tree breeders have worked to produce hybrid trees that might be suitable substitutes for the blight-susceptible American chestnut, Castanea dentate, in timber and nut...

  2. Molecular mapping of resistance to blight in an interspecific cross in the genus Castanea

    Treesearch

    Thomas L. Kubisiak; F.V. Hebard; C. Dana Nelson; Jiansu Zhang; R. Bernatzky; H. Huang; S.L. Anagnostakis; R.L. Doudrick

    1997-01-01

    A three-generation American chestnut x Chinese chestnut pedigree was used to construct a genetic linkage map for chestnut and to investigate the control of resistance to Endothia parasitica (chestnut blight fungus). DNA genotypes for 241 polymorphic markers (eight isozymes, 17 restriction fragment length polymorphisms [RFLPs], and 216 random...

  3. Transgenic American chestnuts show enhanced blight resistance and transmit the trait to T1 progeny.

    PubMed

    Newhouse, Andrew E; Polin-McGuigan, Linda D; Baier, Kathleen A; Valletta, Kristia E R; Rottmann, William H; Tschaplinski, Timothy J; Maynard, Charles A; Powell, William A

    2014-11-01

    American chestnut (Castanea dentata) is a classic example of a native keystone species that was nearly eradicated by an introduced fungal pathogen. This report describes progress made toward producing a fully American chestnut tree with enhanced resistance to the blight fungus (Cryphonectria parasitica). The transgenic American chestnut 'Darling4,' produced through an Agrobacterium co-transformation procedure to express a wheat oxalate oxidase gene driven by the VspB vascular promoter, shows enhanced blight resistance at a level intermediate between susceptible American chestnut and resistant Chinese chestnut (Castanea mollissima). Enhanced resistance was identified first with a leaf-inoculation assay using young chestnuts grown indoors, and confirmed with traditional stem inoculations on 3- and 4-year-old field-grown trees. Pollen from 'Darling4' and other events was used to produce transgenic T1 seedlings, which also expressed the enhanced resistance trait in leaf assays. Outcrossed transgenic seedlings have several advantages over tissue-cultured plantlets, including increased genetic diversity and faster initial growth. This represents a major step toward the restoration of the majestic American chestnut. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  4. American Chestnut Growth and Survival Five Years after Planting in Two Silvicultural Treatments in the Southern Appalachians, USA

    Treesearch

    Stacy L. Clark; Henry Mcnab; David Loftis; Stanley Zarnoch

    2012-01-01

    The ability to restore American chestnut (Castanea dentata) through the planting of blight-resistant (Cryphonectria parasitica) trees is currently being tested. Forest-based research on the species’ silvicultural requirements and chestnut blight development are lacking. Pure American chestnut seedlings were planted in a two-age...

  5. Chapter 12: Reestablishing American chestnut on mined lands in the Appalachian coalfields

    Treesearch

    Michael French; Chris Barton; Brian McCarthy; Carolyn Keiffer; Jeff Skousen; Carl Zipper; Patrick Angel

    2017-01-01

    American chestnut was formerly a major component of forests throughout the Appalachian coalfields and beyond. Chestnut's strong, lightweight wood was naturally rot-resistant, making it a preferred timber tree for many purposes. Unlike many nut-producing trees that flower early in the year, American chestnuts flower in June and July, so they were less susceptible...

  6. Effects of light acclimation on photosynthesis, growth, and biomass allocation in america chestnut seedlings

    Treesearch

    G. Geoff Wang; William L. Bauerle; Bryan T. Mudder

    2006-01-01

    American chestnut [Castanea dentate(Marshall) Borkh.] was a widely distributed tree species in the Eastern U.S., comprising an estimated 25 percent of native eastern hardwood forests. Chestnut blight eradicated American chestnut from the forest canopy by the 1950s, and now it only persists as understory sprouts. However, blight-resistant hybrids with...

  7. Polymorphic sequence-characterized codominant loci in the chestnut blight fungus, Cryphonectria parasitica

    Treesearch

    J. E. Davis; Thomas L. Kubisiak; M. G. Milgroom

    2005-01-01

    Studies on the population biology of the chestnut blight fungus, Cryphonectria parasitica, have previously been carried out with dominant restriction fragment length polymorphism (RFLP) fingerprinting markers. In this study, we described the development of 11 condominant markers from randomly amplified polymorphic DNAs (RAPDs). RAPD fragments were...

  8. Evaluation of chestnut test plantings in the Eastern United States

    Treesearch

    Frederick H. Berry

    1980-01-01

    Between 1947 and 1955, 15 plots were established in the Eastern United States to evaluate chestnut hybrids under forest conditions. During the 1978 field season these test plots were reassessed and all living chestnut trees critically examined. Ten percent of the 250 surviving hybrid chestnuts were blight resistant, and had the timber form and rapid growth of the...

  9. Ectomycorrhizal characterization of an American chestnut (Castanea dentata)-dominated community in Western Wisconsin

    Treesearch

    Jonathan M. Palmer; Daniel L. Lindner; Thomas J. Volk

    2008-01-01

    Circa 1900, a farmer from the eastern US planted 11 American chestnut (Castanea dentata) seeds on a newly established farm near West Salem in western Wisconsin. These trees were very successful, producing a large stand of over 6,000 trees. Since this area is well outside the natural range of chestnut, these trees remained free from chestnut blight...

  10. Electroantennographic responses of the lesser chestnut weevil curculio sayi (Coleoptera: Curculionidae) to volatile organic compounds identified from chestnut reproductive plant tissue

    USDA-ARS?s Scientific Manuscript database

    The primary insect pest of the developing chestnut industry in the central United States is the lesser chestnut weevil, Curculio sayi (Gyllenhal), which is a specialist on only Castanea trees. Recent research has shown this insect is attracted to and feeds upon the reproductive tissues of the chestn...

  11. The efficiency of introduced pisolithus tinctorius inoculum on backcrossed chestnut germination and survival

    Treesearch

    Jenise M. Bauman; Carolyn H. Keiffer; Shiv Hiremath

    2012-01-01

    American chestnut was eliminated as a canopy tree from the Appalachian region of North America with the introduction of chestnut blight in the early 1900s. Breeding programs initiated in the 1980s have produced seedling lines that display the pure American morphology with potential resistance to chestnut blight. More work is required to assess their field performance...

  12. Genetic and genomic resources for mapping resistance to Phytophthora cinnamomi in chestnut

    Treesearch

    T. Zhebentyayeva; A. Chandra; A.G. Abbott; M.E. Staton; B.A. Olukolu; F.V. Hebard; L.L. Georgi; S.N. Jeffers; P.H. Sisco; J.B. James; C. Dana Nelson

    2013-01-01

    Root rot (caused by Phytophthora cinnamomi) and chestnut blight (caused by Cryphonectria parasitica) are the two most destructive diseases affecting American chestnut, Castanea dentata. Therefore, breeding for resistance to both pathogens simultaneously is essential before the American chestnut can be restored to its full native range. Using combined genetic and...

  13. Assessing potential changes of weather-related risk on chestnut productivity

    NASA Astrophysics Data System (ADS)

    Pereira, Mário; Calheiros, Tomas; Pinto, Joaquim; Caramelo, Liliana

    2013-04-01

    Weather conditions play an important role during different phases of the vegetative cycle of the chestnut trees and, consequently, several meteorological parameters seem to be associated chestnut productivity (Heiniger and Conedera, 1992, Cesaraccio et al., 2001, Wilczynski and Podalski, 2007, Gomes-Laranjo et al., 2008, Dinis et al., 2011, Pereira et al., 2011). Observed data from European Climate Assessment and simulated data by COSMO-CLM model for the actual (C20) and future (A1B and B1) climate scenarios were used in this study to: (i) assess the model ability to reproduce weather parameters distribution; and, (ii) to assess future changes in the distribution of meteorological parameters which play an important role in the productivity of chestnut for different future periods. Results points to statistical significant changes in the mean and in variance in the future, more prominent in temperature than in precipitation based parameters. Changes in precipitation will be more significant in Northwestern Iberian Peninsula and France in the end of the 21st century for A1B scenario conditions. As expected, more significant changes will be expected to occur during spring and summer, in the Mediterranean areas and in the later period. The number of days with Tmax<28°C will generally decrease in both scenarios, while the changes in the number of days with 24°C

  14. Horse Chestnut

    MedlinePlus

    ... chestnut Latin Name: Aesculus hippocastanum Background Horse chestnut trees are native to the Balkan Peninsula (which includes ... and swelling after surgery. Preparations made from the tree’s bark are applied to skin sores. Usable parts ...

  15. Survey for the presence of Phytophthora cinnamomi on reclaimed mined lands in Ohio chosen for restoration of the American chestnut

    Treesearch

    Shiv Hiremath; Kirsten Lehtoma; Jenise M. Bauman

    2013-01-01

    We have been planting blight resistant American chestnut seedlings on reclaimed coal mined areas in Southeastern Ohio, which was once within the natural range of the American chestnut. Towards the goal of restoring the American chestnut, we are testing suitable sites that can aid survival, growth and establishment of planted seedlings pre-inoculated with...

  16. Cytogenetic analysis of American chestnut (Castanea dentata) using fluorescent in situ hybridization.

    Treesearch

    MN Islam Faridi; CD Nelson; PH Sisco; TL Kubisiak; FV Hebard; RL Paris; RL Phillips

    2009-01-01

    The American chestnut (Castanea dentata), once known as ‘The King of the Forest’ in the Appalachian Mountains of the eastern United States and southeastern Canada, has been all but extirpated by chestnut blight disease caused by an Asiatic bark fungus, Cryphonectria parasitica. A group of scientists at The American Chestnut Foundation has been working since 1983 to...

  17. Remedial Investigation Work Plan for Chestnut Ridge Operable Unit 1 (Chestnut Ridge Security Pits) at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1994-03-01

    This document outlines the activities necessary to conduct a Remedial Investigation (RI) of the Chestnut Ridge Security Pits (CRSP) at the Oak Ridge Y-12 Plant. The CRSP, also designated Chestnut Ridge Operable Unit (OU) 1, is one of four OUs along Chestnut Ridge on the Oak Ridge Reservation (ORR). The purpose of the RI is to collect data to (1) evaluate the nature and extent of known and suspected contaminants, (2) support an Ecological Risk Assessment (ERA) and a Human Health Risk Assessment (HHRA), (3) support the feasibility study in the development and analysis of remedial alternatives, and (4) ultimately,more » develop a Record of Decision (ROD) for the site. This chapter summarizes the regulatory background of environmental investigation on the ORR and the approach currently being followed and provides an overview of the RI to be conducted at the CRSP. Subsequent chapters provide details on site history, sampling activities, procedures and methods, quality assurance (QA), health and safety, and waste management related to the RI.« less

  18. Salicylic acid inhibits enzymatic browning of fresh-cut Chinese chestnut (Castanea mollissima) by competitively inhibiting polyphenol oxidase.

    PubMed

    Zhou, Dan; Li, Lin; Wu, Yanwen; Fan, Junfeng; Ouyang, Jie

    2015-03-15

    The inhibitory effect and associated mechanisms of salicylic acid (SA) on the browning of fresh-cut Chinese chestnut were investigated. Shelled and sliced chestnuts were immersed in different concentrations of an SA solution, and the browning of the chestnut surface and interior were inhibited. The activities of polyphenol oxidase (PPO) and peroxidase (POD) extracted from chestnuts were measured in the presence and absence of SA. SA at concentrations higher than 0.3g/L delayed chestnut browning by significantly inhibiting the PPO activity (P<0.01), and the POD activity was not significantly affected (P>0.05). The binding and inhibition modes of SA with PPO and POD, determined by AUTODOCK 4.2 and Lineweaver-Burk plots, respectively, established SA as a competitive inhibitor of PPO. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Shoot winter injury and nut cold tolerance: Possible limitations for American chestnut restoration in cold environments? In: Sniezko, Richard A.; Yanchuk, Alvin D.; Kliejunas, John T.; Palmieri, Katharine M.; Alexander, Janice M.; Frankel, Susan J., tech

    Treesearch

    Thomas M. Saielli; Paul G. Schaberg; Gary J. Hawley; Joshua M. Halman; Kendra M. Gurney

    2012-01-01

    Approximately 100 years ago, American chestnut (Castanea dentata (Marsh.) Borkh.) was rapidly removed as an overstory tree by the fungal pathogen Cryphonectria parasitica (the causal agent of chestnut blight). Currently, the most effective method of restoration involves the hybridization of American chestnut with the...

  20. Residues of diflubenzuron on horse chestnut (Aesculus hippocastanum) leaves and their efficacy against the horse chestnut leafminer, Cameraria ohridella.

    PubMed

    Nejmanová, Jana; Cvacka, Josef; Hrdý, Ivan; Kuldová, Jelena; Mertelík, Josef; Muck, Alexander; Nesnerová, Petra; Svatos, Ales

    2006-03-01

    Residues of the insect growth regulator diflubenzuron were quantified on horse chestnut (Aesculus hippocastanum L.) leaves treated with a diflubenzuron 480 g litre(-1) SC, Dimilin. To analyse the samples, an analytical procedure was developed involving a simple extraction step followed by high-performance liquid chromatography on an octadecyl-modified silica column with methanol + 0.01 M ammonium acetate mobile phase. The results showed diflubenzuron to be highly stable on horse chestnut leaves; more than 4 months (127 days) after application, 38% (on average) of the insecticide still remained on/in the leaves. The data confirmed biological observations showing diflubenzuron's long-term efficacy against the horse chestnut leafminer, Cameraria ohridella Deschka and Dimić, which is the most important pest of the horse chestnut in Europe. The hypothesis of possible penetration of diflubenzuron into the leaf mass is explored and discussed.

  1. Nursery quality and first-year response of american chestnut (Castanea dentata) seedlings planted in the southeastern United States

    Treesearch

    Stacy L. Clark; Callie J. Schweitzer; Scott E. Schlarbaum; Luben D. Dimov; Frederick V. Hebard

    2010-01-01

    We examined nursery seedling quality and 1-yr field performance of American chestnut [Castanea dentata (Marshall) Borkh.] seedlings planted in Alabama (AL study) and Tennessee (TN study). Root-collar diameter (RCD) had the highest correlations to nursery seedling quality and first-year fleld performance for both studies. Survival was low in the...

  2. Calendar year 1996 annual groundwater monitoring report for the Chestnut Ridge Hydrogeologic Regime at the U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1997-02-01

    This annual monitoring report contains groundwater and surface water monitoring data obtained in the Chestnut Ridge Hydrogeologic Regime (Chestnut Ridge Regime) during calendar year (CY) 1996. The Chestnut Ridge Regime encompasses a section of Chestnut Ridge west of Scarboro Road and east of an unnamed drainage feature southwest of the US Department of Energy (DOE) Oak Ridge Y-12 Plant (unless otherwise noted, directions are in reference to the Y-12 Plant administrative grid). The Chestnut Ridge Regime contains several sites used for management of hazardous and nonhazardous wastes associated with plant operations. Groundwater and surface water quality monitoring associated with thesemore » waste management sites is performed under the auspices of the Y-12 Plant Groundwater Protection Program (GWPP). Included in this annual monitoring report are the groundwater monitoring data obtained in compliance with the Resource Conservation and Recovery Act (RCRA) Post-Closure Permit for the Chestnut Ridge Regime (post-closure permit) issued by the Tennessee Department of Environment and Conservation (TDEC) in June 1996. Besides the signed certification statement and the RCRA facility information summarized below, condition II.C.6 of the post-closure permit requires annual reporting of groundwater monitoring activities, inclusive of the analytical data and results of applicable data evaluations, performed at three RCRA hazardous waste treatment, storage, or disposal (TSD) units: the Chestnut Ridge Sediment Disposal Basin (Sediment Disposal Basin), the Chestnut Ridge Security Pits (Security Pits), and Kerr Hollow Quarry.« less

  3. Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-κB-signaling Pathway

    PubMed Central

    Kim, Bora; Kim, Jin Eun; Choi, Byung-Kook; Kim, Hyun-Soo

    2015-01-01

    Water chestnut (Trapa japonica Flerov.) is an annual aquatic plant. In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species. In addition, water chestnut extract (WCE) inhibited lipopolysaccharide (LPS)-induced nitric oxide production and suppressed mRNA and protein expression of the inducible nitric oxide synthase gene. The cytokine array results showed that WCE inhibited inflammatory cytokine secretion. Also, WCE reduced tumor necrosis factor-α-and interleukin-6-induced nuclear factor-αB activity. Furthermore, during sodium lauryl sulfate (SLS)-induced irritation of human skin, WCE reduced SLS-induced skin erythema and improved barrier regeneration. These results indicate that WCE may be a promising topical anti-inflammatory agent. PMID:25593649

  4. Early results of a chestnut planting in eastern Kentucky illustrate reintroduction challenges

    Treesearch

    Cornelia C. Pinchot; Scott E. Schlarbaum; Jennifer A. Franklin; David S. Buckley; Stacy L. Clark; Callie J. Schweitzer; Arnold M. Saxton; Frederick V. Hebard

    2012-01-01

    This paper examines the first year results from a silvicultural study of American, hybrid (BC2F3) and Chinese chestnut seedlings (Castanea spp. Mill.) on the Daniel Boone National Forest in southeastern Kentucky. After one year, no significant differences in growth were found among the silvicultural...

  5. Growth of American chestnut and incidence of chestnut blight in the forest understory

    Treesearch

    Amy. Milo

    2010-01-01

    Three hundred individuals of American chestnut, Castanea dentata, at Mountain Lake Biological Station, elevation 1,160 m, were monitored over two field seasons for incidence and growth of cankers caused by the fungus Cryphonectria parasitica.

  6. Functional properties and in vitro antioxidant and antibacterial effectiveness of pigskin gelatin films incorporated with hydrolysable chestnut tannin.

    PubMed

    Peña-Rodriguez, Cristina; Martucci, Josefa F; Neira, Laura M; Arbelaiz, Aitor; Eceiza, Arantxa; Ruseckaite, Roxana A

    2015-04-01

    The impact of the incorporation of 10% w/w of hydrolyzable chestnut tannin into pigskin gelatin (G) films plasticized with glycerol (Gly) on the physicochemical properties as well as the in vitro antioxidant and antibacterial effectiveness against food-borne pathogens such as Escherichia coli and Streptococcus aureus was investigated. A higher tendency to both redness (a*) and yellowness (b*) coloration characterized gelatin films incorporated with chestnut tannin. The reduced lightness (L) and transparency of gelatin-chestnut tannin films plasticized with 30% w/w Gly might be associated with certain degree of phase separation which provoked the migration of the plasticizer to the film surface. The incorporation of chestnut tannin and glycerol affected the chemical structure of the resultant films due to the establishment of hydrogen interactions between components as revealed by Fourier transform infrared spectroscopy. These interactions reduced gelatin crystallinity and seemed to be involved in the substantial decrease of the water uptake of films with tannin, irrespective of the glycerol level. Such interactions had minor effect on tensile properties being similar to those of the control films (without chestnut tannin) at the same glycerol level. Films modified with 10% w/w chestnut tannin showed significant (P < 0.05) 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, ca. from 0 ± 0.033 to 87.1 ± 0.002% for chestnut tannin-free and chestnut tannin-containing gelatin films. The limited inhibitory activity of films incorporated with 10% w/w chestnut tannin against the selected bacteria evidenced by disk diffusion method probably resulted from the interactions within the film restricting the diffusion of the active agent into the agar medium. The more modest protective effect observed against a Gram-positive bacterium (S. aureus) was also discussed. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  7. Evaluation of Calendar Year 1997 Groundwater and Surface Water Quality Data For The Chestnut Ridge Hydrogeologic Regime At The U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jones, S.B.

    1998-09-01

    This report presents an evaluation of the groundwater monitoring data obtained in the Chestnut Ridge Hydrogeologic Regime (Chestnut Ridge Regime) during calendar year (CY) 1997. The Chestnut Ridge Regime encompasses a section of Chestnut Ridge bordered by the U.S. Department of Energy (DOE) Y-12 Plant in Bear Creek Valley (BCV) to the north, Scarboro Road to the eas~ Bethel Valley Road to the south, and an unnamed drainage basin southwest of the Y-12 Plant (Figure 1). Groundwater quality monitoring is performed at hazardous and nonhazardous waste management facilities in the regime under the auspices of the Y-12 Plant Groundwater Protectionmore » Program (GWPP). The CY 1997 monitoring data are presented in Calendar Year 1997 Annual Groundwater Monitoring Report for the Chestnut Ridge Hydrogeolo~"c Regime at the US. Department of Energy Y-12 Plant, Oak Ridge, Tennessee (MA Technical Services, Inc. 1998), which also presents results of site-specific monitoring data evaluations required under the Resource Conservation and Recovery Act (RCIL4) post-closure permit (PCP) for the Chestnut Ridge Regime« less

  8. The American Chestnut Foundation breeding program

    Treesearch

    F.V. Hebard

    2012-01-01

    Chestnut blight, incited by Cryphonectria parasistica, devastated American chestnut (Castanea dentata (Borkh.) Marsh) in the first half of the 20th century, killing approximately 4 billion dominant and codominant trees. Millions of small sprouts still persist throughout the botanical range of C. dentata. Most are not infected...

  9. American chestnut persistence in southwestern Virginia 80 years after chestnut blight introduction

    Treesearch

    Katie L. Burke

    2010-01-01

    Forest disease noticeably alters spatial patterns of a species' distribution and this alteration is complex when host mortality is affected by site qualities. In the 1930s, chestnut blight (Cryphonectria parasitica) spread through southwestern Virginia, after its introduction to New York in 1904.

  10. Effects of simulated prescribed fire on American chestnut and northern red oak regeneration

    Treesearch

    Ethan P. Belair; Mike R. Saunders; Stacy L. Clark

    2014-01-01

    American chestnut (Castanea dentata [Marsh.] Borkh.) was a dominant species in the forests of eastern North America prior to the importation of chestnut blight (Cryphonectria parasitica [Murr.] Barr) in the early 1900s and ink disease (Phytophthora cinnamomi Rands) in the 1800s (Anagnostakis 2012). Historical...

  11. Silvicultural and logistical considerations associated with the pending reintroduction of American chestnut

    Treesearch

    Douglass F. Jacobs

    2010-01-01

    Traditional breeding for blight resistance has led to the potential to restore American chestnut (Castanea dentata (Marsh.) Borkh.) to Eastern United States forests using a blight resistant hybrid chestnut tree. With prospects of pending wide-scale reintroduction, restoration strategies based on ecological and biological characteristics of the...

  12. Early results from a pilot test of planting small American chesnut seedlings under a forest canopy

    Treesearch

    W. Henry McNab; Steven Patch; A. Amelia Nutter

    2003-01-01

    Successful development of American chestnut (Castanea dentata) hybrids that are resistant to chestnut blight (Cryphonectria parasitica) will require information about methods for effective and economical reintroduction of this species in forests of the southern Appalachian Mountains (Boucher 2000) American chestnut regenerates...

  13. 97. Catalog B, Higher Plants, 200 2 American Chestnut Tree, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    97. Catalog B, Higher Plants, 200 2 American Chestnut Tree, Negative No. 6032 (Photographer and date unknown) THIS GHOST FOREST OF BLIGHTED CHESTNUTS ONCE STOOD APPROXIMATELY AT THE LOCATION OF THE BYRD VISITOR CENTER. - Skyline Drive, From Front Royal, VA to Rockfish Gap, VA , Luray, Page County, VA

  14. Lipid composition of Castanea sativa Mill. and Aesculus hippocastanum fruit oils.

    PubMed

    Zlatanov, Magdalen D; Antova, Ginka A; Angelova-Romova, Maria J; Teneva, Olga T

    2013-02-01

    Sweet and horse chestnut fruit contain carbohydrates, fibers, proteins, lipids, vitamins, glycosides and coumarin. The lipids are rich in biologically active substances as fatty acids, phospholipids, sterols and tocopherols. The fruit has been used as food, and for medicinal purposes to treat inflammatory and vascular problems. The fruits of sweet and horse chestnut contain 20 and 81 g kg(-1) glyceride oil respectively. The content of phospholipids in the oils was 49 and 3 g kg(-1). Sterols were found to be 8 and 12 g kg(-1). In the tocopherol fraction (1920 and 627 mg kg(-1)) γ-tocopherol predominated in the sweet chestnut oil (927 g kg(-1)); γ-tocopherol (591 g kg(-1)) and α-tocopherol (402 g kg(-1)) in horse chestnut oil. Palmitic, oleic and linoleic acids predominated in the triacylglycerols. Higher quantities of palmitic and oleic acids were established in the phospholipids and sterol esters. The fruits of horse and sweet chestnut have a close lipid composition. The oils are rich in essential fatty acids, such as linoleic and linolenic, as well as biologically active substances: phospholipids, sterols and tocopherols. This fact determines the good food value of sweet chestnut fruit and the possibilities for use of horse chestnuts in pharmacy and for technical purposes. © 2012 Society of Chemical Industry.

  15. Flavonoids in horse chestnut (Aesculus hippocastanum) seeds and powdered waste water byproducts.

    PubMed

    Kapusta, Ireneusz; Janda, Bogdan; Szajwaj, Barbara; Stochmal, Anna; Piacente, Sonia; Pizza, Cosimo; Franceschi, Federico; Franz, Chlodwig; Oleszek, Wieslaw

    2007-10-17

    Horse chestnut extracts are widely used in pharmacy and cosmetic industries. The main active constituents are saponins of oleane type, but seeds of horse chestnut also contain flavonoids, being glycosides of quercetin and kaempferol. Their contribution to the overall activity of the extracts was not clear. In the present work, the main flavonoids from horse chestnut seeds were isolated and their structures established with spectral methods. Seven glycosides were isolated, out of which six ( 2, 3, 4, 7, 11, 13) were previously reported and one ( 9) was identified as a new tamarixetin 3- O- [beta- d-glucopyranosyl(1-->3)]- O-beta- d-xylopyranosyl-(1-->2)- O-beta- d-glucopyranoside. The structures of three additional compounds 1, 10, and 12, not previously reported, were deduced on the basis of their LC-ESI/MS/MS fragmentation characteristics. A new ultraperformance liquid chromatographic (UPLC) method has been developed for profiling and quantitation of horse chestnut flavonoids. The method allowed good separation over 4.5 min. Thirteen compounds could be identified in the profile, out of which di- and triglycoisdes of quercetin and kaempferol were the dominant forms and their acylated forms occurred in just trace amounts. The total concentration of flavonoids in the powdered horse chestnut seed was 0.88% of dry matter. The alcohol extract contained 3.46%, and after purification on C18 solid phase, this concentration increased to 9.40% of dry matter. The flavonoid profile and their content were also measured in the horse chestnut wastewater obtained as byproduct in industrial processing of horse chestnut seeds. The total flavonoid concentration in the powder obtained after evaporation of water was 2.58%, while after purification on solid phase, this increased to 11.23% dry matter. It was concluded that flavonoids are present in a horse chestnut extract in a relatively high amount and have the potential to contribute to the overall activity of these extracts. Industrial horse chestnut wastewater can be used to obtain quercetine and kaempferol glycosides for cosmetic, nutraceutical, and food supplement industries.

  16. The effect of feed moisture and temperature on tannin content, antioxidant and antimicrobial activities of extruded chestnuts.

    PubMed

    Obiang-Obounou, Brice Wilfried; Ryu, Gi Hyung

    2013-12-15

    This study focuses on the effect of extrusion processing on tannin reduction, phenolic content, flavonoid content, antioxidant and anitimicrobial activity. Extrusion temperature (120 and 140 °C) and feed moisture (25% and 28%) were used on the tannin content, antioxidant and antimicrobial activities. Extrusion cooking reduced tannin content up to 78%, and improved antioxidant activity from 12.89% to 21.17% in a concentration dependant manner without affecting its antimicrobial activity that varied from 250 to 500 mg. The time-kill assay confirmed the ability of extruded chestnut to reduce Pseudomonas aeruginosa count below detectable limit that reduced the original inoculum by 3log10 CFU/mL. Overall, the results showed that extrusion cooking might serve as a tool for tannin reduction and could improve the antioxidant and antimicrobial properties of chestnut, which might be helpful for chestnut related products in the food industry. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Production of fermented chestnut purees by lactic acid bacteria.

    PubMed

    Blaiotta, G; Di Capua, M; Coppola, R; Aponte, M

    2012-09-03

    The objective of this study was to develop a new chestnut-based puree, in order to seasonally adjust the offer and use the surplus of undersized production, providing, at the same time, a response to the growing demand for healthy and environmentally friendly products. Broken dried chestnuts have been employed to prepare purees to be fermented with six different strains of Lactobacillus (Lb.) rhamnosus and Lactobacillus casei. The fermented purees were characterized by a technological and sensorial point of view, while the employed strains were tested for their probiotic potential. Conventional in vitro tests have indicated the six lactobacilli strains as promising probiotic candidates; moreover, being the strains able to grow and to survive in chestnut puree at a population level higher than 8 log₁₀ CFU/mL along 40 days of storage at 4 °C, the bases for the production of a new food, lactose-free and with reduced fat content, have been laid. Copyright © 2012 Elsevier B.V. All rights reserved.

  18. Recovery of bioactive molecules from chestnut (Castanea sativa Mill.) by-products through extraction by different solvents.

    PubMed

    Vella, Filomena Monica; Laratta, Bruna; La Cara, Francesco; Morana, Alessandra

    2018-05-01

    The underutilised forest and industrial biomass of Castanea sativa (Mill.) is generally discarded during post-harvest and food processing, with high impact on environmental quality. The searching on alternative sources of natural antioxidants from low-cost supplies, by methods involving environment-friendly techniques, has become a major goal of numerous researches in recent times. The aim of the present study was the set-up of a biomolecules extraction procedure from chestnut leaves, burs and shells and the assessing of their potential antioxidant activity. Boiling water was the best extraction solvent referring to polyphenols from chestnut shells and burs, whereas the most efficient for leaves resulted 60% ethanol at room temperature. Greatest polyphenol contents were 90.35, 60.01 and 17.68 mg gallic acid equivalents g -1 in leaves, burs and shells, respectively. Moreover, flavonoids, tannins and antioxidant activity were assessed on the best extract obtained from each chestnut by-product.

  19. Bur and nut production on three chestnut cultivars

    Treesearch

    Michele Warmund; Darin J. Enderton; J.W. Van Sambeek

    2010-01-01

    Studies were conducted to characterize bur and nut development on shoots of young chestnut (Castanea sp.) trees over a two year period and to determine the effect of secondary (2°) bur removal on subsequent bur and nut production. Terminal shoots of 'Peach' trees with primary (1°) and 2° burs (PS) grew longer and...

  20. Potential of ultrasonic pulse velocity for evaluating the dimensional stability of oak and chestnut wood

    Treesearch

    Turker Dundar; Xiping Wang; Nusret As; Erkan Avci

    2016-01-01

    The objective of this study was to examine the potential of ultrasonic velocity as a rapid and nondestructive method to predict the dimensional stability of oak (Quercus petraea (Mattuschka) Lieblein) and chestnut (Castanea sativa Mill.) that are commonly used in flooring industry. Ultrasonic velocity, specific gravity, and radial, tangential and volumetric shrinkages...

  1. Facilitation of American chestnut (Castanea dentata) seedling establishment by Pinus virginiana in mine restoration

    Treesearch

    Jenise M. Bauman; Carolyn H. Keiffer; Shiv Hiremath

    2012-01-01

    This study evaluated the influence of planting sites on the establishment and ectomycorrhizal (ECM) colonization of American chestnut (Castanea denetata (Marsh.) Borkh.) on an abandoned coal mine in an Appalachian region of the United States. Root morphotyping and sequencing of the fungal internal transcribed spacer (ITS) region were used to identify...

  2. The influence of inoculated and native ectomycorrhizal fungi on morphology, physiology and survival of American chestnut

    Treesearch

    Jenise M. Bauman; Carolyn H. Keiffer; Shiv Hiremath

    2011-01-01

    The objective of this study was to evaluate the influence of five different species of ectomycorrhizal (ECM) fungi on root colonization of native fungi on putatively blight resistant chestnut hybrids (Castanea dentata x C. mollissima) in a reclaimed mine site in central Ohio. The five species were Hebeloma crustuliniforme, Laccaria bicolor,...

  3. Genetic variation patterns of American chestnut populations at EST-SSRs

    Treesearch

    Oliver Gailing; C. Dana Nelson

    2017-01-01

    The objective of this study is to analyze patterns of genetic variation at genic expressed sequence tag - simple sequence repeats (EST-SSRs) and at chloroplast DNA markers in populations of American chestnut (Castanea dentata Borkh.) to assist in conservation and breeding efforts. Allelic diversity at EST-SSRs decreased significantly from southwest to northeast along...

  4. Using DNA Markers to Distinguish Among Chestnut Species and Hybrids

    Treesearch

    Thomas L. Kubisiak

    1999-01-01

    Identification of American chestnut trees in the wild for inclusion in breeding programs is currently done using morphological traits. Distinguishing traits include leafshape, stipule size, presence or absence of leaf and stem trichomes, and stem color. Application of these traits is reasonably clear if the trees are pure American chestnut, but identitication of...

  5. Mortality, early growth, and blight occurrence in hybrid, Chinese, and American chestnut seedlings in West Virginia

    Treesearch

    Melissa Thomas-Van Gundy; Jane Bard; Jeff Kochenderfer; Paul Berrang

    2017-01-01

    Two plantings of second (BC3F2) and third (BC3F3) backcross generations of hybrid American chestnuts established in east-central West Virginia were assessed after 4 years to determine family effects on growth and survival. Pure American and pure Chinese chestnut seedlings were...

  6. Nursery performance of American and Chinese chestnuts and backcross generations in commercial tree nurseries

    Treesearch

    Stacy L. Clark; Scott E. Schlarbaum; Arnold M. Saxton; Fred V. Hebard

    2012-01-01

    The American chestnut [Castanea dentata (Marsh.) Borkh.] was decimated by an exotic fungus [Cryphonectria parasitica (Murr.) Barr] in the early 1900s. Breeding efforts with American and Chinese chestnuts (C. mollissima Blume) produced putatively blight-resistant progeny (BC3F3) in 2007. We compared two nut size...

  7. Reintroduction of American Chestnut in the National Forest System

    Treesearch

    Stacy L. Clark; Scott E. Schlarbaum; Cornelia C. Pinchot; Sandra L. Anagnostakis; Michael R. Saunders; Melissa Thomas-Van Gundy; Paul Schaberg; James McKenna; Jane F. Bard; Paul C. Berrang; David M. Casey; Chris E. Casey; Barbara Crane; Brian D. Jackson; Jeff D. Kochenderfer; Russ MacFarlane; Robert Makowske; Mark D. Miller; Jason A. Rodrigue; Jim Stelick; Christopher D. Thornton; Tyler S. Williamson

    2014-01-01

    American chestnut restoration depends on a multitude of biological, administrative, and technological factors. Germplasm traditionally bred for resistance to the chestnut blight disease caused by the exotic pathogen Cryphonectria parasitica has been deployed on national forests in the Eastern and Southern Regions of the National Forest System (NFS) since 2009. Trees...

  8. Inadequate cold tolerance as a possible limitation to American chestnut restoration in the northeastern United States

    Treesearch

    Kendra M. Gurney; Paul G. Schaberg; Gary Hawley; John B. Shane

    2011-01-01

    The American chestnut (Castanea dentata (Marshall) Borkh.), once a major component of eastern forests from Maine to Georgia, was functionally removed from the forest ecosystem by chestnut blight (an exotic fungal disease caused by Cryphonectria parasitica (Murr.) Barr), first identified at the beginning of the twentieth century....

  9. Ocular Injury With Chestnut Burr: Our Experience.

    PubMed

    García-García, Gerardo P; Esaa-Caride, Neyla C; Jurado-Guano, Nancy D; Muñoz-Bellido, Luis

    2016-10-01

    To evaluate the medical and surgical management and treatment of ocular injuries with chestnut burr recorded at the Hospital El Bierzo between 2009 and 2015. A prospective study of ocular injuries with chestnut burr was conducted for the period from 2009 to 2015. Patients were detected in the Emergency Department and referred to the Ophthalmology Department for study and medical and surgical treatment. Ten eyes were evaluated in 10 patients. The injuries ranged from ocular contusion without corneal involvement to trauma with corneal perforation caused by spines. One patient with chronic retention of spines in the intrastromal corneal ring was included. Six of the eyes studied had chestnut burr spines in the cornea, one of which was chronically retained in the intrastromal cornea. One case of spine-induced corneal perforation was also studied. Six of the patients required surgery, 2 required removal of the spine using a slit lamp, 2 required outpatient medical treatments, and a wait-and-see approach was taken in 1 patient. All patients progressed favorably, and no surgical complications were recorded. Proper occupational risk prevention and the use of eye protection glasses are fundamental in avoiding these accidents. Once they have occurred, medical and surgical management must be done early and on an individual basis depending on the severity and location of the injuries, to avoid potentially serious complications.

  10. The influence of silvicultural treatments and site conditions on American chestnut (Castanea dentata) seedling establishment in eastern Kentucky, USA

    Treesearch

    Chuck Rhoades; David Loftis; Jeffrey Lewis; Stacy Clark

    2009-01-01

    After more than 50 years of research and selective breeding, blight-resistant American chestnut (Castanea dentata) trees will soon be available for planting into the species' pre-blight range. Increased understanding of the regeneration requirements of pure American chestnut (C. dentata [Marsh.] Borkh.) will increase the...

  11. American Chestnut, Rhododendron, and the Future Of Appalachian Cove Forests

    Treesearch

    David H. van Lear; D.B. Vandermast; C.T. Rivers; T.T. Baker; C.W. Hedman; B.D. Clinton; T.A. Waldrop

    2002-01-01

    Abstract - By the mid 1930s, the southern Appalachians had been heavily cutover and the dominant hardwood, American chestnut (Castanea dentata), had succumbed to the chestnut blight (Cryphonectria parasitica). Forests that had been burned on a frequent basis for millennia were now protected and fire was excluded in large degree. We estimated the pre-...

  12. Soil preparation methods promoting ectomycorrhizal colonization and American chestnut Castanea dentata establishment in coal mine restoration

    Treesearch

    Jenise M. Bauman; Carolyn H. Keiffer; Shiv Hiremath; Brian C. McCarthy

    2013-01-01

    The objective of this research was to evaluate soil subsurface methods that may aid in seedling establishment and encourage root colonization from a diverse group of ectomycorrhizal (ECM) fungi during restoration projects. American chestnut Castanea dentata Marsh. Borkh. and backcrossed chestnuts seedlings were planted on a reclaimed coal mine site...

  13. Lessons from the field: The first tests of restoration American chestnut (Castanea dentata) seedlings planted in the Southern Region

    Treesearch

    Stacy Clark; Scott Schlarbaum; John Saxton; Fred Hebard; John Blanton; David Casey; Barbara Crane; Russ MacFarlane; Jason Rodrigue; Stelick Jim

    2012-01-01

    An exotic fungus, the chestnut blight (Cryphonectria parasitica Murr. Barr), decimated the American chestnut tree (Castanea dentata Marsh. Borkh.) throughout eastern North America in the first half of the 20th century. The United States Department of Agriculture, Forest Service (FS), The University of Tennessee, and The American...

  14. Restoring the American chestnut tree

    Treesearch

    Bryan Burhans; Fredrick V. Hebard

    2012-01-01

    The American chestnut (Castanea dentata) was a dominate hardwood tree in the eastern United States. Its historic range extended from Maine south to the northern parts of Georgia, Alabama, Mississippi, and west to the Ohio River Valley. In 1904, an exotic Asian fungus responsible for the death of American chestnut trees was first identified at the Bronx Zoo (New York...

  15. Exploration of a rare population of Chinese chestnut in North America: stand dynamics, health and genetic relationships

    Treesearch

    Amy C. Miller; Keith E. Woeste; Sandra L. Anagnostakis; Doutlass F. Jacobs

    2014-01-01

    With the transport of plants around the globe, exotic species can readily spread disease to their native relatives; however, they can also provide genetic resistance to those relatives through hybrid breeding programmes. American chestnut (Castanea dentata) was an abundant tree species in North America until its decimation by introduced chestnut...

  16. Molecular markers linked to resistance to Cryphonectria parasitica in chestnut

    Treesearch

    Thomas L. Kubisiak

    1996-01-01

    Kubisiak describes how he came to work on the chestnut blight problem. He touches on the underlying theory behind recombinational linkage mapping, mentions some current results in work with chestnut, and discusses how these results compare to prior knowledge regarding the suspected pattern of inheritance of blight resistance. Finally, the author looks ahead and...

  17. Biology of the European oak borer in Michigan, United States of America, with comparisons to the native twolined chestnut borer

    Treesearch

    Toby R. Petrice; Robert A. Haack

    2014-01-01

    In 2010-2011, we studied the European oak borer (EOB), Agrilus sulcicollis Lacordaire (Coleoptera: Buprestidae), in Michigan, United States of America, and made comparisons with the native twolined chestnut borer (TLCB), Agrilus bilineatus (Weber). EOB adult flight began and peaked before TLCB. More EOB females were captured on...

  18. Hepatoprotective Potential of Chestnut Bee Pollen on Carbon Tetrachloride-Induced Hepatic Damages in Rats

    PubMed Central

    Yıldız, Oktay; Can, Zehra; Saral, Özlem; Yuluğ, Esin; Öztürk, Ferhat; Aliyazıcıoğlu, Rezzan; Canpolat, Sinan; Kolaylı, Sevgi

    2013-01-01

    Bee pollen has been used as an apitherapy agent for several centuries to treat burns, wounds, gastrointestinal disorders, and various other diseases. The aim of our study was to investigate the hepatoprotective effects of chestnut bee pollen against carbon tetrachloride (CCI4)-induced liver damage. Total phenolic content, flavonoid, ferric reducing/antioxidant power, and DPPH radical activity measurements were used as antioxidant capacity determinants of the pollen. The study was conducted in rats as seven groups. Two different concentrations of chestnut bee pollens (200 and 400 mg/kg/day) were given orally and one group was administered with silibinin (50 mg/kg/day, i.p.) for seven days to the rats following the CCI4 treatment. The protective effect of the bee pollen was monitored by aspartate transaminase (AST) and alanine transaminase (AST) activities, histopathological imaging, and antioxidant parameters from the blood and liver samples of the rats. The results were compared with the silibinin-treated and untreated groups. We detected that CCI4 treatment induced liver damage and both the bee pollen and silibinin-treated groups reversed the damage; however, silibinin caused significant weight loss and mortality due, severe diarrhea in the rats. The chestnut pollen had showed 28.87 mg GAE/g DW of total phenolic substance, 8.07 mg QUE/g DW of total flavonoid, 92.71 mg Cyn-3-glu/kg DW of total anthocyanins, and 9 mg β-carotene/100 g DW of total carotenoid and substantial amount of antioxidant power according to FRAP and DPPH activity. The results demonstrated that the chestnut bee pollen protects the hepatocytes from the oxidative stress and promotes the healing of the liver damage induced by CCI4 toxicity. Our findings suggest that chestnut bee pollen can be used as a safe alternative to the silibinin in the treatment of liver injuries. PMID:24250716

  19. Sourdough fermentation and chestnut flour in gluten-free bread: A shelf-life evaluation.

    PubMed

    Rinaldi, Massimiliano; Paciulli, Maria; Caligiani, Augusta; Scazzina, Francesca; Chiavaro, Emma

    2017-06-01

    The effect of sourdough fermentation combined with chestnut flour was investigated for improving technological and nutritional quality of gluten-free bread during 5day shelf life by means of chemico-physical and nutritional properties. Sourdough fermentation by itself and with chestnut flour reduced volume of loaves and heterogeneity in crumb grain. Sourdough technology allowed increasing crumb moisture content with no significant variations during shelf-life. Chestnut flour darkened crumb and crust while no effects on colour were observed for sourdough. Sourdough and/or chestnut flour addition caused a significant increase in crumb hardness at time 0 while a significant reduction of staling was observed only at 5days, even if a decrease in amylopectin fusion enthalpy was observed. The percentage of hydrolysed starch during in vitro digestion was significantly reduced by sourdough fermentation with a presumable lower glycaemic index. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Chestnut green waste composting for sustainable forest management: Microbiota dynamics and impact on plant disease control.

    PubMed

    Ventorino, Valeria; Parillo, Rita; Testa, Antonino; Viscardi, Sharon; Espresso, Francesco; Pepe, Olimpia

    2016-01-15

    Making compost from chestnut lignocellulosic waste is a possible sustainable management strategy for forests that employs a high-quality renewable organic resource. Characterization of the microbiota involved in composting is essential to better understand the entire process as well as the properties of the final product. Therefore, this study investigated the microbial communities involved in the composting of chestnut residues obtained from tree cleaning and pruning. The culture-independent approach taken highlighted the fact that the microbiota varied only slightly during the process, with the exception of those of the starting substrate and mature compost. The statistical analysis indicated that most of the bacterial and fungal species in the chestnut compost persisted during composting. The dominant microbial population detected during the process belonged to genera known to degrade recalcitrant lignocellulosic materials. Specifically, we identified fungal genera, such as Penicillium, Fusarium, Cladosporium, Aspergillus and Mucor, and prokaryotic species affiliated with Bacilli, Actinobacteria, Flavobacteria and γ-Proteobacteria. The suppressive properties of compost supplements for the biocontrol of Sclerotinia minor and Rhizoctonia solani were also investigated. Compared to pure substrate, the addition of compost to the peat-based growth substrates resulted in a significant reduction of disease in tomato plants of up to 70 % or 51 % in the presence of Sclerotinia minor or Rhizoctonia solani, respectively. The obtained results were related to the presence of putative bio-control agents and plant growth-promoting rhizobacteria belonging to the genera Azotobacter, Pseudomonas, Stenotrophomonas, Bacillus, Flavobacterium, Streptomyces and Actinomyces in the chestnut compost. The composting of chestnut waste may represent a sustainable agricultural practice for disposing of lignocellulosic waste by transforming it into green waste compost that can be used to improve the fitness of agricultural plants. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Restoration of chestnuts as a timber crop in Connecticut

    Treesearch

    S.L. Anagnostakis; C.C. Pinchot

    2014-01-01

    American chestnut trees were an important source of timber in Connecticut until chestnut blight disease reduced them to understory shrubs. Breeding begun in 1930 has now produced trees with enough resistance to initiate field trials in the forest. Biological control by hypovirulence viruses is being used in the plots in an effort to keep native trees alive. If native...

  2. Performance of container-grown seedlings of American chestnut backcross hybrids BC3 F3 generation in central Louisiana

    Treesearch

    Shi-Jean Susana Sung; Stacy L. Clark; Scott Schlarbaum; Daniel C. Dey; Daniel J. Leduc

    2016-01-01

    Seedlings from two families of the BC3F3 backcross generation of the American chestnut (Castanea dentata) and Chinese chestnut (C. mollissima) were cultured in 2013 in Missouri using the Root Production Method®, a container-based system used to avoid disease problems associated with...

  3. Physicochemical properties and in vitro digestibility of starch from naturally air-dried chestnut.

    PubMed

    Zhao, Jinkai; Zhang, Yuyang; Wu, Yanwen; Liu, Lingling; Ouyang, Jie

    2018-06-08

    Naturally air-dried chestnut is a type of traditionally processed chestnut in North China which has a pleasant flavor. After air drying at room temperature and low-air humidity for two wk, the moisture, total starch content and starch relative crystallinity decreased, while the content of water-soluble sugar and amylose increased because of the dehydration and the hydrolysis of endogenous amylase. The Fourier transform infrared (FTIR) spectroscopy ratio of 1047/1022 cm -1 and the relative area of the Raman spectrum peak at 480/865 cm -1 of air-dried chestnut starch decreased in the first two wk and then increased, while the full width at half-maximum height (FWHH) of the Raman spectrum peak at 480 cm -1 showed the opposite tendency. Crystallinity had a positive correlation with the springiness and chewiness, and was negatively correlated with the estimated glycemic index (eGI). The eGI of air-dried starch was lower than those of roasted or boiled starch, which indicated that naturally air-dried chestnut with low digestibility is a good alternative to thermally processed chestnut. Copyright © 2017. Published by Elsevier B.V.

  4. Remedial Investigation Report on Chestnut Ridge Operable Unit 2 (Filled Coal Ash Pond/Upper McCoy Branch) at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. Volume 1. Main Text

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1994-08-01

    This document is a report on the remedial investigation (RI) of Chestnut Ridge Operable Unit (OU) 2 at the Oak Ridge Y-12 Plant. Chestnut Ridge OU 2 consists of Upper McCoy Branch (UMB), the Filled Coal Ash Pond (FCAP), and the area surrounding the Sluice Channel formerly associated with coal ash disposal in the FCAP. Chestnut Ridge OU 2 is located within the U.S. Department of Energy`s (DOE`s) Oak Ridge Reservation in Anderson County, Tennessee, approximately 24 miles west of Knoxville. The pond is an 8.5-acre area on the southern slope of Chestnut Ridge, 0.5 mile south of the mainmore » Y-12 Plant and geographically separated from the Y-12 Plant by Chestnut Ridge. The elevation of the FCAP is {approximately} 950 ft above mean sea level (msl), and it is relatively flat and largely vegetated. Two small ponds are usually present at the northeast and northwest comers of the FCAP. The Sluice Channel Area extends {approximately}1000 ft from the northern margin of the FCAP to the crest of Chestnut Ridge, which has an elevation of {approximately}1100 ft above msl. The Sluice Channel Area is largely vegetated also. McCoy Branch runs from the top of Chestnut Ridge across the FCAP into Rogers Quarry and out of the quarry where it runs a short distance into Milton Hill Lake at McCoy Embayment, termed UMB. The portion south of Rogers Quarry, within Chestnut Ridge OU 4, is termed Lower McCoy Branch. The DOE Oak Ridge Y-12 Plant disposed of coal ash from its steam plant operations as a slurry that was discharged into an ash retention impoundment; this impoundment is the FCAP. The FCAP was built in 1955 to serve as a settling basin after coal ash slurried over Chestnut Ridge from the Y-12 Plant. The FCAP was constructed by building an earthen dam across the northern tributary of McCoy Branch. The dam was designed to hold 20 years of Y-12 steam plant ash. By July 1967, ash had filled up the impoundment storage behind the dam to within 4 ft of the top.« less

  5. Preparation and characterisation of the oligosaccharides derived from Chinese water chestnut polysaccharides.

    PubMed

    Wu, Sheng-Jun; Yu, Lin

    2015-08-15

    Hydrogen peroxide (H2O2) is a strong oxidant that cleaves glycosidic bonds in polysaccharides. In this study, the oligosaccharides were prepared by removing the starch from Chinese water chestnuts through hydrolysis using α-amylase and then hydrolysing the remaining polysaccharides with H2O2, during which the oligosaccharide yield was monitored. The yield of oligosaccharide was affected by reaction time, temperature, and H2O2 concentration. Extended reaction times, high temperatures, and high H2O2 concentrations decreased oligosaccharide yield. Under optimum conditions (i.e., reaction time of 4h, reaction temperature of 80°C, and 2.5% H2O2 concentration), the maximum oligosaccharide yield was 3.91%. The oligosaccharides derived from Chinese water chestnuts polysaccharides exhibited strong hydroxyl and 2,2-diphenyl-β-picrylhydrazyl radical scavenging activity when applied at a concentration of 100 μg/mL. The results indicate that the oligosaccharides derived from Chinese water chestnuts polysaccharides possessed good antioxidant properties and can be developed as a new dietary supplement and functional food. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Hydrolyzable Tannins from Sweet Chestnut Fractions Obtained by a Sustainable and Eco-friendly Industrial Process.

    PubMed

    Campo, Margherita; Pinelli, Patrizia; Romani, Annalisa

    2016-03-01

    Sweet Chestnut (Castanea sativa Mill.) wood extracts, rich in Hydrolyzable Tannins (HTs), are traditionally used in the tanning and textile industries, but recent studies suggest additional uses. The aim of this work is the HPLC-DAD-ESI-MS characterization of Sweet Chestnut aqueous extracts and fractions obtained through a membrane separation technology system without using other solvents, and the evaluation of their antioxidant and antiradical activities. Total tannins range between 2.7 and 138.4 mM; gallic acid ranges between 6% and 100%; castalagin and vescalagin range between 0% and 40%. Gallic Acid Equivalents, measured with the Folin-Ciocalteu test, range between 0.067 and 56.99 g/100 g extract weight; ORAC test results for the marketed fractions are 450.4 and 3050 µmol/g Trolox Equivalents/extract weight. EC₅₀ values, measured with the DPPH test, range between 0.444 and 2.399 µM. These results suggest a new ecofriendly and economically sustainable method for obtaining chestnut fractions with differentiated, stable and reproducible chemical compositions. Such fractions can be marketed for innovative uses in several sectors.

  7. Incidence of Craesus castaneae (Hymenoptera: Tenthredinidae) on Chestnut Seedlings Planted in the Daniel Boone National Forest, Kentucky

    Treesearch

    Cornelia C. Pinchot; Scott E. Schlarbaum; Arnold M. Saxton; Stacy L. Clark; Callie J. Schweitzer; David R. Smith; Alex. Mangini; Frederick V. Hebard

    2011-01-01

    American chestnut [Castanea dentate (Marshall) Borkhausen, Fagales: Fagaceae] was a dominant forest tree in the eastern forests of the U.S. until it was eliminated as a canopy tree species by 2 exotic pathogens. Ink disease, a root rot caused by Phytophthora cinnamomi Rands (Pythiales: Pythiaceae), began to destroy chestnut populations on bottomland and poorly-drained...

  8. Is nut cold tolerance a limitation to the restoration of American chestnut in the northeastern United States?

    Treesearch

    Paul G. Schaberg; Kendra M. Gurney; Benjamin R. Janes; Joshua M. Halman; Gary J. Hawley

    2009-01-01

    American chestnut (Castanea dentata) was once a dominant hardwood species in the eastern United States, growing from Maine to Georgia and west to the Ohio Valley (Harlow et al. 1979). Arguably, American chestnut may have been the most important hardwood species in North America, renowned for its quick growth, massive size, and great utility (Harlow...

  9. Proceedings, USDA Forest Service American Chestnut cooperators' meeting

    Treesearch

    H. Clay Smith; William L. MacDonald

    1982-01-01

    The American chestnut was an important tree in the history of the United States. The fruit of chestnut was not only important to man and his domesticated animals but to the wildlife of the eastern forests. The tree comprised over 25 percent of the eastern hardwood forest and its natural range included at least 200 million acres. On good sites, the tree often grew 1...

  10. Effectiveness of a detached‐leaf assay as a proxy for stem inoculations in backcrossed chestnut (Castanea) blight resistance breeding populations

    Treesearch

    N. R. LaBonte; J.R. McKenna; K. Woeste

    2016-01-01

    A recently developed detached-leaf blight resistance assay has generated interest because it could reduce the amount of time needed to evaluate backcrossed hybrid trees in the American chestnut blight resistance breeding programme. We evaluated the leaf inoculation technique on a sample of advanced progeny from the Indiana state chapter American Chestnut Foundation...

  11. The chestnut oak forests of the anthracite region

    Treesearch

    C. F. Burnham; M. J. Ferree; F. E. Cunningham

    1947-01-01

    The chestnut oak forests occur mostly on poor sites along the tops and southern slopes of ridges in the central and southern parts of the Anthracite Region (see map). This forest type is not of much commercial value. It contains some saw timber and mine timber, but most of the chestnut oak stands are of seedling-and-sapling size. Furthermore, many of them are in...

  12. Proceedings of the American chestnut symposium

    Treesearch

    William L. MacDonald; Franklin C. Cech; John Luchok; Clay Smith

    1978-01-01

    The year was 1904; the place the Bronx Zoological Park in New York City; the beginning of perhaps the greatest single natural catastrophe in the annals of forest history—the discovery of chestnut blight. In less than 50 years more than 80 percent of the American chestnut trees in the eastern hardwood forests were dead; the rest were dying. A tree species that once...

  13. Life in the cold - another challenge to American chestnut restoration?

    Treesearch

    Kenda Gurney; Paul Schaberg

    2010-01-01

    The restoration of the American chestnut is a goal that uniltes chestnut enthusiasts from Maine to Georgia, from the East Coast to the Ohio River, and even beyond the boundaries of this majestic species' native range. But while our goal is the same--to restore this tree to its former place in the forest--the obstacles vary with each location this effort is...

  14. Tannin analysis of chestnut bark samples (Castanea sativa Mill.) by HPLC-DAD-MS.

    PubMed

    Comandini, Patrizia; Lerma-García, María Jesús; Simó-Alfonso, Ernesto Francisco; Toschi, Tullia Gallina

    2014-08-15

    In the present investigation, an HPLC-DAD/ESI-MS method for the complete analysis of tannins and other phenolic compounds of different commercial chestnut bark samples was developed. A total of seven compounds (vescalin, castalin, gallic acid, vescalagin, 1-O-galloyl castalagin, castalagin and ellagic acid) were separated and quantified, being 1-O-galloyl castalagin tentatively identified and found for the first time in chestnut bark samples. Thus, this method provided information regarding the composition and quality of chestnut bark samples, which is required since these samples are commercialised due to their biochemical properties as ingredients of food supplements. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Searching for American chestnut: the estimation of rare species attributes in a national forest inventory

    Treesearch

    Francis A. Roesch; William H. McWilliams

    2007-01-01

    American chestnut, once a dominant tree species in forests of the Northeastern United States, has become extremely rare. It is so rare, in fact, that on completion of 80 percent of the plot measurements of the U.S. Department of Agriculture Forest Service's most recent inventory in Pennsylvania, only 33 American chestnut trees with a diameter at breast height !Y 1...

  16. Searching for American chestnut: the estimation of rare species attributes in a national forest inventory

    Treesearch

    Francis A. Roesch; William H. McWilliams

    2005-01-01

    American chestnut, once a dominant tree species in forests of the Northeastern United States, has become extremely rare. It is so rare, in fact, that on completion of 80 percent of the plot measurements of the U.S. Department of Agriculture Forest Service's most recent inventory in Pennsylvania, only 33 American chestnut trees with a diameter at breast height 2: 1...

  17. Influence of Soil Type and Drainage on Growth of Swamp Chestnut Oak (Quercus Michauxii Nutt.) Seedlings

    Treesearch

    Donald D. Hook

    1969-01-01

    Swamp chestnut oak (Quercus michauxii Nutt.) seedlings were grown for 2 years in five soil types in drained and undrained pots. First-year height growth was related to soil type and pot drainage, but second-year height growth was related only to soil type. Results suggest that swamp chestnut oak is site-sensitive. But slow growth, a maximum of 2...

  18. Short-term storage evaluation of quality and antioxidant capacity in chestnut-wheat bread.

    PubMed

    Rinaldi, Massimiliano; Paciulli, Maria; Dall'Asta, Chiara; Cirlini, Martina; Chiavaro, Emma

    2015-01-01

    Bread traditionally made from wheat is now often supplemented with alternative functional ingredients as chestnut flours; no data have been previously published about the staling of chestnut-containing bread. Thus short-term storage (3 days) for chestnut flour-supplemented soft wheat bread is evaluated by means of selected physicochemical properties (i.e. water dynamics, texture, colour, crumb grain characteristic, total antioxidant capacity). Bread prepared with a 20:80 ratio of chestnut:soft wheat flours maintained its moisture content in both crust and crumb. Crumb hardness, after baking, was found to be significantly higher than that of the soft wheat bread; it did not change during storage, whereas it significantly increased in the control bread until the end of the shelf life. The supplemented bread presented a heterogeneous crumb structure, with a significant decrease in the largest pores during shelf life, relative to the shrinkage of crumb grain. The control exhibited a significant redistribution of crumb holes, with a decrease in the smallest grain classes and an increase in the intermediate ones, most likely caused by cell wall thickening. The colour of the crumb remained unaltered in both breads. The crust of the control presented a significant decrease of a* (redness) and that of the supplemented bread exhibited a decrease of b* (yellowness). The antioxidant capacity was detected after day 1 of storage in the chestnut flour bread only. Chestnut flour supplementation could represent a feasible way of producing bread with improved characteristics, not only just after baking but also during shelf life. © 2014 Society of Chemical Industry.

  19. Multitemporal WorldView satellites imagery for mapping chestnut trees

    NASA Astrophysics Data System (ADS)

    Marchetti, F.; Arbelo, M.; Moreno-Ruíz, J. A.; Hernández-Leal, P. A.; Alonso-Benito, A.

    2017-10-01

    Chestnuts have been part of the landscape and popular culture of the Canary Islands (Spain) since the sixteenth century. Many crops of this species are in state of abandonment and an updated mapping for its study and evaluation is needed. This work proposes the elaboration of this cartography using two satellite images of very high spatial resolution captured on two different dates and representing well-differentiated phenological states of the chestnut: a WorldView-2 image of March 10th, 2015 and a WorldView-3 image of May 12th, 2015 (without and with leaves respectively). Two study areas were selected within the municipality of La Orotava (Tenerife Island). One of the areas contains chestnut trees dispersed in an agricultural and semi-urban environment and in the other one, the specimens are grouped forming a forest merged with Canarian pines and other species of Monteverde. The Maximum Likelihood (ML), the Artificial Neural Networks (ANN) and the Spectral Angle Mapper (SAM) classification algorithms were applied to the multi-temporal image resulting from the combination of both dates. The results show the benefits of using the multi-temporal image for Pinolere with the ANN algorithm and for Chasna area with ML algorithm, in both cases providing an overall accuracy close to 95%.

  20. Application of a new purification method of West-Kazakhstan chestnut soil microbiota DNA for metagenomic analysis

    NASA Astrophysics Data System (ADS)

    Sergaliev, N. Kh.; Kakishev, M. G.; Zhiengaliev, A. T.; Volodin, M. A.; Andronov, E. E.; Pinaev, A. G.

    2015-04-01

    A method for the extraction of soil microbial DNA has been tested on chestnut soils (Kastanozems) of the West Kazakhstan region. The taxonomic analysis of soil microbiome libraries has shown that the phyla Actinobacteria and Proteobacteria constitute the largest part of microbial communities in the analyzed soils. The Archaea form an appreciable part of the microbiome in the studied samples. In the underdeveloped dark chestnut soil, their portion is higher than 11%. This is of interest, as the proportion of Archaea in the soil communities of virgin lands usually does not exceed 5%. In addition to the phyla mentioned above, there are representatives of the phyla Acidobacteria, Bacteroidetes, Firmicutes, Gemmatimonadales, Planctomycetes, and Verrucomicrobia, which are all fairly common in soil communities.

  1. Antibacterial and antimycotic activities of Slovenian honeys.

    PubMed

    Kuncic, M Kralj; Jaklic, D; Lapanje, A; Gunde-Cimerman, N

    2012-01-01

    In the present study, Slovenian honey samples produced from different floral sources are evaluated for their antibacterial and antifungal properties. The peroxide contribution to antibacterial activity is also determined. Minimum inhibitory concentration (MIC) of the honeys was assessed against four bacterial species (Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus) and against eight fungal species (Aspergillus niger, Aureobasidium pullulans, Candida albicans, Candida parapsilosis, Candida tropicalis, Cladosporium cladosporioides, Penicillium chrysogenum and Rhodotorula mucilaginosa). Honey at concentrations between 1% and 50% (v/v) were tested. Although all of the bacterial species were inhibited by the different honey samples, the chestnut and pasture honeys showed the highest antibacterial activities. The antifungal activities were concentration-dependent, with five (Aureobasidium pullulans, Candida parapsilosis, Candida tropicalis, Cladosporium cladosporioides, Rhodotorula mucilaginosa) inhibited only at honey concentrations greater than 50%. The fungi Aspergillus niger, Candida albicans and Penicillium chrysogenum were not inhibited by any of the tested honeys, even at the highest concentrations. The lowest MICs seen were 2.5% (v/v) for the chestnut, fir and forest honeys against Staphylococcus aureus, and 10.0% (v/v) for the chestnut and pasture honeys against Cladosporium cladosporioides. The non-peroxide action of chestnut honey was tested against Escherichia coli. The MIC of the catalase-treated chestnut honey was 50% (v/v). The antibacterial effect of Slovenian honeys is mostly due to peroxide action. These data support the concept that Slovenian honeys are effective antibacterials and antifungals, and can thus be applied for medicinal purposes.

  2. Saprophytic Activity and Sporulation of Cryphonectria parasitica on Dead Chestnut Wood in Forests with Naturally Established Hypovirulence.

    PubMed

    Prospero, S; Conedera, M; Heiniger, U; Rigling, D

    2006-12-01

    ABSTRACT Sustainable biological control of the chestnut blight fungus Crypho-nectria parasitica with hypovirulence depends on the production and dissemination of hypovirus-infected propagules of the pathogen. We investigated the ability of C. parasitica to sporulate and produce hypo-virus-infected spores on recently dead chestnut wood in coppice stands in southern Switzerland where hypovirulence has been naturally established. The number and type (active, inactive, or none) of cankers was assessed on experimentally cut and stacked stems, firewood stacks, and natural dead wood. Hypovirus-free and hypovirus-infected strains readily survived for more than 1 year in the chestnut blight cankers of the stacked stems. Sporulation of C. parasitica was observed on the surface of preexisting inactive and active cankers, as well as on newly colonized bark areas and was significantly more abundant than on comparable cankers on living stems. On all types of dead wood, we observed more stromata with perithecia than with pycnidia; however, a large proportion of the stromata was not differentiated. All perithecia examined yielded only hypovirus-free ascospores. The incidence of pycnidia that produced hypovirus-infected conidia ranged from 5% on natural dead wood to 41% on the experimental stacks. The mean virus transmission rate into conidia was 69%. Our study demonstrates a considerable saprophytic activity of C. parasitica on recently dead chestnut wood and supports the hypothesis of a role of this saprophytic phase in the epidemiology of hypovirulence.

  3. Antiinflammatory effect of Japanese horse chestnut (Aesculus turbinata) seeds.

    PubMed

    Sato, Itaru; Kofujita, Hisayoshi; Suzuki, Tadahiko; Kobayashi, Haruo; Tsuda, Shuji

    2006-05-01

    The antiinflammatory effects of Japanese horse chestnut (Aesculus turbinata) seeds were examined in vivo and in vitro. The extract of this seed (HCSE) inhibited croton oil-induced swelling of the mouse concha. HCSE inhibited cyclooxygenase (COX) -1 and -2 activities, but had no effect on 15-lipoxygenase and phospholipase A2 activities. Inhibition of COX-2 occurred at a lower concentration of HCSE than for COX-1. Japanese horse chestnut seeds contain coumarins and saponins, but these chemicals did not inhibit COX activities. These results suggest that the antiinflammatory effect of Japanese horse chestnut seeds is caused, at least partly, by the inhibition of COX. The inhibitor of COX in this seed may be a chemical(s) other than coumarins and saponins.

  4. A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys.

    PubMed

    Kolayli, Sevgi; Can, Zehra; Yildiz, Oktay; Sahin, Huseyin; Karaoglu, Sengul Alpay

    2016-01-01

    This study was planned to investigate some physicochemical and anti-inflammatory, antioxidant, antimicrobial properties of three different degrees of unifloral characters of chestnut honeys. Antihyaluronidase, antiurease and antimicrobial activities were evaluated as anti-inflammatory characteristics. Total phenolic contents, flavonoids, tannins, phenolic profiles, ferric-reducing antioxidant power (FRAP), scavenging activities of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS + ) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals were evaluated as antioxidant properties. Color, optical rotation, conductivity, moisture, pH and ash content were evaluated as physicochemical parameters, and some sugars content, prolin, diastase, HMF and minerals (Na, K, Ca, P, Fe, Cu and Zn) were evaluated as chemical and biochemical parameters. All studied physicochemical and biological active properties were changed in line with the unifloral character of the chestnut honeys. A higher unifloral character was found associated with greater apitherapeutic capacity of the honey, as well as biological active compounds.

  5. Antioxidant activity of yogurt made from milk characterized by different casein haplotypes and fortified with chestnut and sulla honeys.

    PubMed

    Perna, Annamaria; Intaglietta, Immacolata; Simonetti, Amalia; Gambacorta, Emilio

    2014-11-01

    The aim of this work was to evaluate the antioxidant activity of yogurt made from milk characterized by different casein (CN) haplotypes (αs1-, β-, κ-CN) and fortified with chestnut and sulla honeys. The CN haplotype was determined by isoelectric focusing, whereas antioxidant activity of yogurt was measured using 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid and ferric-reducing antioxidant power. The statistical analysis showed a significant effect of the studied factors. The results showed that chestnut honey presented the highest phenolic acid and flavonoid contents, which are closely associated with its high antioxidant activity. The antioxidant activity of fortified yogurt samples was affected both by different CN haplotypes and by type of honey added. Yogurts fortified with chestnut honey showed higher antioxidant activity than those fortified with sulla honey. The different behavior observed among the fortified yogurts led us to hypothesize that the effects of protein-polyphenol complex on antioxidant activity are interactive. The results suggest that milk proteins polymorphism and polyphenols play different roles in affecting the bioavailability and the antioxidant activity of yogurt. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  6. [Intoxication by powdered seeds of horse chestnut (Aesculus hippocastanum) used nasally as snuff - a case report].

    PubMed

    Zając, Maciej; Wiśniewski, Marek; Sein Anand, Jacek

    2014-01-01

    There are only few reports in the medical literature about side effects and toxicity of horse chestnut (Aesculus hippocastanum). We report a 15-year-old woman who was admitted to the hospital because of symptoms including: vomiting, dyspnea, burning in the nose and throat, and syncope, after intranasal snuff of powdered horse chestnut seeds. Laboratory tests showed no abnormalities. After 2 days of hospitalization the female was discharged home with subjective and objective improvement. Preparation and use of snuff is related to the tradition of the kashubian region. The powder formed from horse chestnuts, which is white in color, effects after about 5-10 minutes, and causes severe irritation of the nasal mucous membranes, which results in sneezing. Responsible for side effects is mainly aescin. The most frequently observed aescin intoxication symptoms were gastrointestinal irritation and allergic reactions. Intoxication by powdered seeds of horse chestnut used nasally as snuff may lead, as it was in our case, to sudden and self-limiting clinical symptoms. Supportive therapy and a short hospital observation seems to be sufficient in such cases.

  7. Development of methodology for identification the nature of the polyphenolic extracts by FTIR associated with multivariate analysis

    NASA Astrophysics Data System (ADS)

    Grasel, Fábio dos Santos; Ferrão, Marco Flôres; Wolf, Carlos Rodolfo

    2016-01-01

    Tannins are polyphenolic compounds of complex structures formed by secondary metabolism in several plants. These polyphenolic compounds have different applications, such as drugs, anti-corrosion agents, flocculants, and tanning agents. This study analyses six different type of polyphenolic extracts by Fourier transform infrared spectroscopy (FTIR) combined with multivariate analysis. Through both principal component analysis (PCA) and hierarchical cluster analysis (HCA), we observed well-defined separation between condensed (quebracho and black wattle) and hydrolysable (valonea, chestnut, myrobalan, and tara) tannins. For hydrolysable tannins, it was also possible to observe the formation of two different subgroups between samples of chestnut and valonea and between samples of tara and myrobalan. Among all samples analysed, the chestnut and valonea showed the greatest similarity, indicating that these extracts contain equivalent chemical compositions and structure and, therefore, similar properties.

  8. 342. BAPTIZED PENTECOSTAL CHURCH AT 1606 WEST CHESTNUT STREET, EAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    342. BAPTIZED PENTECOSTAL CHURCH AT 1606 WEST CHESTNUT STREET, EAST SIDE - Russell Neighborhood, Bounded by Congress & Esquire Alley, Fifteenth & Twenty-first Streets, Louisville, Jefferson County, KY

  9. The Forest Health Initiative, American chestnut (Castanea dentata) as a model for forest tree restoration: Biological Research Program

    Treesearch

    C. Dana Nelson; W.A. Powell; C.A. Maynard; K.M. Baier; A. Newhouse; S.A. Merkle; C.J. Nairn; L. Kong; J.E. Carlson; C. Addo-Quaye; M.E. Staton; F.V. Hebard; L.L. Georgi; A.G. Abbott; B.A. Olukolu; T. Zhebentyayeva

    2013-01-01

    The Forest Health Initiative (FHI) was developed and implemented to test the hypothesis that a coordinated effort in biotechnology research could lead to resistant trees capable of restoring a species in a relevant time frame. As a test case, the American chestnut (Castanea dentata) was chosen for study as it is an iconic forest tree species in the eastern United...

  10. 300. VACANT LOTS BETWEEN WEST MADISON ALLEY AND WEST CHESTNUT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    300. VACANT LOTS BETWEEN WEST MADISON ALLEY AND WEST CHESTNUT STREET, TOWARD WEST - Russell Neighborhood, Bounded by Congress & Esquire Alley, Fifteenth & Twenty-first Streets, Louisville, Jefferson County, KY

  11. 5. Historic American Buildings Survey, December, 1959 DETAIL OF WINDOW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey, December, 1959 DETAIL OF WINDOW AND IRON GRILLE, FIRST FLOOR, CHESTNUT STREET FACADE. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  12. Identification and expression analysis of starch branching enzymes involved in starch synthesis during the development of chestnut (Castanea mollissima Blume) cotyledons

    PubMed Central

    Li, Zhi; Zhao, Yanyan; Jiang, Yichen; Zhang, Qing; Cao, Qingqin; Fang, Kefeng; Xing, Yu; Qin, Ling

    2017-01-01

    Chinese chestnut (Castanea mollissima Blume) is native to China and distributes widely in arid and semi-arid mountain area with barren soil. As a perennial crop, chestnut is an alternative food source and acts as an important commercial nut tree in China. Starch is the major metabolite in nuts, accounting for 46 ~ 64% of the chestnut dry weight. The accumulation of total starch and amylopectin showed a similar increasing trend during the development of nut. Amylopectin contributed up to 76% of the total starch content at 80 days after pollination (DAP). The increase of total starch mainly results from amylopectin synthesis. Among genes associated with starch biosynthesis, CmSBEs (starch branching enzyme) showed significant increase during nut development. Two starch branching enzyme isoforms, CmSBE I and CmSBE II, were identified from chestnut cotyledon using zymogram analysis. CmSBE I and CmSBE II showed similar patterns of expression during nut development. The accumulations of CmSBE transcripts and proteins in developing cotyledons were characterized. The expressions of two CmSBE genes increased from 64 DAP and reached the highest levels at 77 DAP, and SBE activity reached its peak at 74 DAP. These results suggested that the CmSBE enzymes mainly contributed to amylopectin synthesis and influenced the amylopectin content in the developing cotyledon, which would be beneficial to chestnut germplasm selection and breeding. PMID:28542293

  13. Anthraquinones isolated from the browned Chinese chestnut kernels (Castanea mollissima blume)

    NASA Astrophysics Data System (ADS)

    Zhang, Y. L.; Qi, J. H.; Qin, L.; Wang, F.; Pang, M. X.

    2016-08-01

    Anthraquinones (AQS) represent a group of secondary metallic products in plants. AQS are often naturally occurring in plants and microorganisms. In a previous study, we found that AQS were produced by enzymatic browning reaction in Chinese chestnut kernels. To find out whether non-enzymatic browning reaction in the kernels could produce AQS too, AQS were extracted from three groups of chestnut kernels: fresh kernels, non-enzymatic browned kernels, and browned kernels, and the contents of AQS were determined. High performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) methods were used to identify two compounds of AQS, rehein(1) and emodin(2). AQS were barely exists in the fresh kernels, while both browned kernel groups sample contained a high amount of AQS. Thus, we comfirmed that AQS could be produced during both enzymatic and non-enzymatic browning process. Rhein and emodin were the main components of AQS in the browned kernels.

  14. Identification of COX inhibitors in the hexane extract of Japanese horse chestnut (Aesculus turbinata) seeds.

    PubMed

    Sato, Itaru; Kofujita, Hisayoshi; Tsuda, Shuji

    2007-07-01

    Japanese horse chestnut (Aesculus turbinata) seed extract inhibits the activity of cyclooxygenase (COX), but its active constituents have not been identified. In the present study, COX inhibitors were isolated from the hexane extract of this seed by means of 4 steps of liquid chromatography and were identified by gas chromatography/mass spectrometry and nuclear magnetic resonance. The COX inhibitors in the extract of Japanese horse chestnut seeds were identified as linoleic acid, linolenic acid, and oleic acid. Their efficacies were in the following order: linolenic acid = linoleic acid > oleic acid. These active constituents are C18 unsaturated fatty acids; stearic acid, a C18 saturated fatty acid, had no activity. Linolenic acid and linoleic acid had high selectivity toward COX-2 (selectivity index = 10), whereas oleic acid had no selectivity. Considering the efficacy and yield of each fatty acid, linoleic acid may be the principal COX inhibitor in this seed.

  15. Development of methodology for identification the nature of the polyphenolic extracts by FTIR associated with multivariate analysis.

    PubMed

    Grasel, Fábio dos Santos; Ferrão, Marco Flôres; Wolf, Carlos Rodolfo

    2016-01-15

    Tannins are polyphenolic compounds of complex structures formed by secondary metabolism in several plants. These polyphenolic compounds have different applications, such as drugs, anti-corrosion agents, flocculants, and tanning agents. This study analyses six different type of polyphenolic extracts by Fourier transform infrared spectroscopy (FTIR) combined with multivariate analysis. Through both principal component analysis (PCA) and hierarchical cluster analysis (HCA), we observed well-defined separation between condensed (quebracho and black wattle) and hydrolysable (valonea, chestnut, myrobalan, and tara) tannins. For hydrolysable tannins, it was also possible to observe the formation of two different subgroups between samples of chestnut and valonea and between samples of tara and myrobalan. Among all samples analysed, the chestnut and valonea showed the greatest similarity, indicating that these extracts contain equivalent chemical compositions and structure and, therefore, similar properties. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Honey as an apitherapic product: its inhibitory effect on urease and xanthine oxidase.

    PubMed

    Sahin, Huseyin

    2016-01-01

    The aim of this study was to evaluate new natural inhibitor sources for the enzymes urease and xanthine oxidase (XO). Chestnut, oak and polyfloral honey extracts were used to determine inhibition effects of both enzymes. In addition to investigate inhibition, the antioxidant capacities of these honeys were determined using total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity assays. Due to their high phenolic content, chestnut and oak honeys are found to be a powerful source for inhibition of both enzymes. Especially, oak honeys were efficient for urease inhibition with 0.012-0.021 g/mL IC50 values, and also chestnut honeys were powerful for XO inhibition with 0.028-0.039 g/mL IC50 values. Regular daily consumption of these honeys can prevent gastric ulcers deriving from Helicobacter pylori and pathological disorders mediated by reactive oxygen species.

  17. Chestnut flowers as functionalizing agents to enhance the antioxidant properties of highly appreciated traditional pastry.

    PubMed

    Carocho, Márcio; Barreira, João C M; Bento, Albino; Morales, Patricia; Ferreira, Isabel C F R

    2014-11-01

    Some studies have proven the antioxidant and antimicrobial potency of chestnut flowers both in the raw matrix and after extraction, and the consumption of their decoctions has been related to beneficial effects towards health. In recent years, due to controversy and ambiguous legislation of chemical conservatives, plant extracts have been successfully used as functionalizing agents in different matrixes by displaying their various beneficial effects towards the foodstuff and/or the consumer. In this paper, decoctions of chestnut flowers as well as the dried flower were added to Portuguese traditional cakes that were then stored for 15 and 30 days, after which they were analysed for their antioxidant potential. The results were analysed by means of a 2 way ANOVA and a linear discriminant analysis, concluding that storage time had a slightly higher influence on alteration of the antioxidant activity. DPPH and TBARS were the most improved parameters, regardless of the concentration added.

  18. Sexually mature transgenic American chestnut trees via embryogenic suspension-based transformation.

    PubMed

    Andrade, Gisele M; Nairn, Campbell J; Le, Huong T; Merkle, Scott A

    2009-09-01

    The availability of a system for direct transfer of anti-fungal candidate genes into American chestnut (Castanea dentata), devastated by a fungal blight in the last century, would offer an alternative or supplemental approach to conventional breeding for production of chestnut trees resistant to the blight fungus and other pathogens. By taking advantage of the strong ability of embryogenic American chestnut cultures to proliferate in suspension, a high-throughput Agrobacterium tumefaciens-mediated transformation protocol for stable integration of foreign genes into the tree was established. Proembryogenic masses (PEMs) were co-cultivated with A. tumefaciens strain AGL1 harboring the plasmid pCAMBIA 2301, followed by stringent selection with 50 or 100 mg/l Geneticin. A protocol employing size-fractionation to enrich for small PEMs to use as target material and selection in suspension culture was applied to rapidly produce transgenic events with an average efficiency of four independent transformation events per 50 mg of target tissue and minimal escapes. Mature somatic embryos, representing 18 transgenic events and derived from multiple American chestnut target genotypes, were germinated and over 100 transgenic somatic seedlings were produced and acclimatized to greenhouse conditions. Multiple vigorous transgenic somatic seedlings produced functional staminate flowers within 3 years following regeneration.

  19. Effect of the inclusion of chestnut in the finishing diet on volatile compounds during the manufacture of dry-cured "Lacón" from Celta pig breed.

    PubMed

    Lorenzo, José M; Franco, Daniel; Carballo, Javier

    2014-01-01

    The effect of the finishing diet on the volatile compounds throughout the manufacture of dry-cured "lacón" (a Spanish traditional meat product), from the Celta pig breed was studied. Thirty-six pigs were separated into three groups according to the type of feeding during the finish-fattening period of three months (concentrate, mixed diet and chestnut). From the pigs of each diet, four batches of dry-cured "lacón" were manufactured. From each batch, samples of fresh meat, meat after salting, after post-salting, and after 14, 28, 56 and 84 days of drying-ripening were taken. Volatiles were extracted by a purge-and-trap method and analyzed by gas chromatographic/mass spectrometry (GC/MS). Seventy-six volatile compounds were identified and quantified from dry-cured "lacón" samples in pigs finished with chestnut, eighty-two for concentrate fed pigs and eighty in pigs fed with the mixed diet. The number of identified volatile compounds increased during the manufacturing process; at 84 days of drying-ripening, in the dry-cured "lacón" samples from pigs finished with concentrate, mixed diet and chestnut, 54, 58 and 62 volatile compounds were detected, respectively. The most abundant group of flavour compounds at the end of the manufacturing process was hydrocarbons in the three feeding systems, followed by aldehydes, ketones and alcohols. Discriminant analysis selected six variables (dodecane, butadienol, pentenol, 2-pentenal, decen-3-ona and pyridine-2-methyl) and calculated two discriminating functions which allowed verification of chestnut in the finishing diet. © 2013.

  20. The reintroduction of the American Chestnut

    Treesearch

    Stacy L Clark

    2013-01-01

    Successful reintroduction of the American chestnut will require far more than blight resistance. The greatest challenge will be the ability of blight-resistant seedlings to survive and reproduce in a forest that presents both native and non-native threats

  1. Repeated Small Bowel Obstruction Caused by Chestnut Ingestion without the Formation of Phytobezoars.

    PubMed

    Satake, Ryu; Chinda, Daisuke; Shimoyama, Tadashi; Satake, Miwa; Oota, Rie; Sato, Satoshi; Yamai, Kiyonori; Hachimori, Hisashi; Okamoto, Yutaka; Yamada, Kyogo; Matsuura, Osamu; Hashizume, Tadashi; Soma, Yasushi; Fukuda, Shinsaku

    2016-01-01

    A small number of cases of small bowel obstruction caused by foods without the formation of phytobezoars have been reported. Repeated small bowel obstruction due to the ingestion of the same food is extremely rare. We present the case of 63-year-old woman who developed small bowel obstruction twice due to the ingestion of chestnuts without the formation of phytobezoars. This is the first reported case of repeated small bowel obstruction caused by chestnut ingestion. Careful interviews are necessary to determine the meal history of elderly patients and psychiatric patients.

  2. Airborne castanea pollen forecasting model for ecological and allergological implementation.

    PubMed

    Astray, G; Fernández-González, M; Rodríguez-Rajo, F J; López, D; Mejuto, J C

    2016-04-01

    Castanea sativa Miller belongs to the natural vegetation of many European deciduous forests prompting impacts in the forestry, ecology, allergological and chestnut food industry fields. The study of the Castanea flowering represents an important tool for evaluating the ecological conservation of North-Western Spain woodland and the possible changes in the chestnut distribution due to recent climatic change. The Castanea pollen production and dispersal capacity may cause hypersensitivity reactions in the sensitive human population due to the relationship between patients with chestnut pollen allergy and a potential cross reactivity risk with other pollens or plant foods. In addition to Castanea pollen's importance as a pollinosis agent, its study is also essential in North-Western Spain due to the economic impact of the industry around the chestnut tree cultivation and its beekeeping interest. The aim of this research is to develop an Artificial Neural Networks for predict the Castanea pollen concentration in the atmosphere of the North-West Spain area by means a 20years data set. It was detected an increasing trend of the total annual Castanea pollen concentrations in the atmosphere during the study period. The Artificial Neural Networks (ANNs) implemented in this study show a great ability to predict Castanea pollen concentration one, two and three days ahead. The model to predict the Castanea pollen concentration one day ahead shows a high linear correlation coefficient of 0.784 (individual ANN) and 0.738 (multiple ANN). The results obtained improved those obtained by the classical methodology used to predict the airborne pollen concentrations such as time series analysis or other models based on the correlation of pollen levels with meteorological variables. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Nondestructive detection of infested chestnuts based on NIR spectroscopy

    USDA-ARS?s Scientific Manuscript database

    Insect feeding is a significant postharvest problem for processors of Chestnuts (Castanea sativa, Miller). In most cases, damage from insects is 'hidden', i.e. not visually detectable on the fruit surface. Consequently, traditional sorting techniques, including manual sorting, are generally inadequa...

  4. Biotechnology of trees: Chestnut

    Treesearch

    C.D. Nelson; W.A. Powell; S.A. Merkle; J.E. Carlson; F.V. Hebard; N Islam-Faridi; M.E. Staton; L. Georgi

    2014-01-01

    Biotechnology has been practiced on chestnuts (Castanea spp.) for many decades, including vegetative propagation, controlled crossing followed by testing and selection, genetic and cytogenetic mapping, genetic modifi cation, and gene and genome sequencing. Vegetative propagation methods have ranged from grafting and rooting to somatic embryogenesis, often in...

  5. Efficacy of horse chestnut leaf extract ALH-L1005 as a matrix metalloproteinase inhibitor in ligature-induced periodontitis in canine model

    PubMed Central

    Kim, Se Eun; Kim, Tae Hyun; Park, Shin Ae; Kim, Won Tae; Park, Young Woo; Ahn, Jae Sang; Jeong, Manbok; Kim, Min-Young

    2017-01-01

    Matrix metalloproteinases (MMPs) are the main proteinases associated with periodontal tissue destruction and remodeling. Therefore, inhibition of host-derived MMPs has a key role in the prevention and reduction of periodontitis progression. Horse chestnut (Aesculus hippocastanum L.) extracts have been used as treatments for inflammatory disease, traditionally. This study assessed the clinical effect as a MMP inhibitor of horse chestnut leaf extract ALH-L1005 on periodontitis. ALH-L1005 was obtained from horse chestnut leaf and its MMP inhibitory activities estimated. Periodontitis was induced in beagles assigned to 4 groups and medicated for 6 weeks: low dose test (LT; ALH-L1005, 100 mg/kg/day), high dose test (HT; ALH-L1005, 200 mg/kg/day), positive control (PC; doxycycline, 10 mg/kg/day), or negative control (NC; placebo). Before and after administration, clinical indices of the teeth and MMP quantity in gingival tissues using zymography were measured. Clinical conditions of the LT, HT, and PC groups were significantly improved after 6 weeks. In zymographic evaluations, gelatinolytic and caseinolytic activities were suppressed in LT, HT, and PC groups but not in the NC group. The results suggest that ALH-L1005 could be an effective agent for clinical prevention and treatment of periodontitis by inhibiting the gelatinase and collagenase activities, which can detach periodontal ligaments from alveolar bone. PMID:27515267

  6. Efficacy of horse chestnut leaf extract ALH-L1005 as a matrix metalloproteinase inhibitor in ligature-induced periodontitis in canine model.

    PubMed

    Kim, Se Eun; Kim, Tae Hyun; Park, Shin Ae; Kim, Won Tae; Park, Young Woo; Ahn, Jae Sang; Jeong, Manbok; Kim, Min-Young; Seo, Kangmoon

    2017-06-30

    Matrix metalloproteinases (MMPs) are the main proteinases associated with periodontal tissue destruction and remodeling. Therefore, inhibition of host-derived MMPs has a key role in the prevention and reduction of periodontitis progression. Horse chestnut ( Aesculus hippocastanum L.) extracts have been used as treatments for inflammatory disease, traditionally. This study assessed the clinical effect as a MMP inhibitor of horse chestnut leaf extract ALH-L1005 on periodontitis. ALH-L1005 was obtained from horse chestnut leaf and its MMP inhibitory activities estimated. Periodontitis was induced in beagles assigned to 4 groups and medicated for 6 weeks: low dose test (LT; ALH-L1005, 100 mg/kg/day), high dose test (HT; ALH-L1005, 200 mg/kg/day), positive control (PC; doxycycline, 10 mg/kg/day), or negative control (NC; placebo). Before and after administration, clinical indices of the teeth and MMP quantity in gingival tissues using zymography were measured. Clinical conditions of the LT, HT, and PC groups were significantly improved after 6 weeks. In zymographic evaluations, gelatinolytic and caseinolytic activities were suppressed in LT, HT, and PC groups but not in the NC group. The results suggest that ALH-L1005 could be an effective agent for clinical prevention and treatment of periodontitis by inhibiting the gelatinase and collagenase activities, which can detach periodontal ligaments from alveolar bone.

  7. Effects of environmental parameters on the chestnut gall wasp and its complex of indigenous parasitoids

    NASA Astrophysics Data System (ADS)

    Bonsignore, Carmelo Peter; Bernardo, Umberto

    2018-04-01

    The chestnut gall wasp (CGW), Dryocosmus kuriphilus, an invasive pest native to China, has caused severe yield and economic losses to chestnut production in Europe since its arrival in 2002. In Southern Italy, the complex of indigenous parasitoids colonizing CGW was monitored between 2013 and 2015, with the aim of estimating the composition of the indigenous parasitoid complex, its ability to control CGW populations, and the interactions of both factors with several measured environmental parameters. We compared results among three differently managed field types. Results showed an increase in the rate of parasitism both when the host population density was lower and in unmanaged chestnut stands with more natural conditions. The percentage of parasitism in galls was related to morphological traits of the galls and to higher seasonal temperatures, which reduced the parasitism intensity because CGW develops earlier under such conditions. The host-parasitoid mortality inside galls varied among sites and was associated mostly with rot fungi during wet spring and summer months. Parasitoid species richness was similar among the study sites, but the proportion of parasitoid species differed between orchards and unmanaged coppice stands. The timing of attack by parasitoids followed a species-specific successional sequence throughout the larva-to-adult life cycle of the CGW. These interactions should be considered in future research on trophic relationships and when modeling invasive scenarios for new pest species.

  8. Effect of tannins on the in vitro growth of Clostridium perfringens.

    PubMed

    Elizondo, Ana M; Mercado, Elsa C; Rabinovitz, Bettina C; Fernandez-Miyakawa, Mariano E

    2010-10-26

    Vegetable tannins are water-soluble polyphenolic compounds of varying molecular weights that occur abundantly in nature. The diet of many free-ranging wild animals contains significant amounts of tannins. Also, commercial tannins are used in animal industry as food additives to improve animal performance. In order to further determine the capacity of tannins to inhibit the development of intestinal diseases produced by Clostridium pefringens, we evaluated here the effect of tannins from quebracho, chestnut or combinations of both on C. perfringens and their toxins. The C. perfringens (types A, B, C, D and E) growth obtained from the intestine of healthy and diseased animals was reduced in a dose-dependent manner in the presence of quebracho tannins, chestnut tannins, combinations of both or a commercial formula based in these tannins. Although the minimal inhibitory concentration of both tannins varied between isolates, no statistically significant differences were observed between isolates from healthy or sick animals. Comparative analysis showed that the concentrations of quebracho tannin inhibiting the growth of C. perfringens were higher than chestnut tannin. In fact, antibacterial effect of quebracho tannin was increased up to 20 times with the addition of 25% of chestnut tannin and 85 times with 75% of chestnut tannin. Antibacterial activity of the commercial product was up to ~50 times higher than quebracho tannin alone. Quebracho tannin showed partial bactericidal activity, whereas chestnut tannin activity was stronger. Both tannins were able to reduce the alpha toxin lecithinase activity and epsilon toxin cytotoxicity in MDCK cells. These results suggest that tannin-supplemented diet could be useful to prevent some clostridial diseases. Copyright © 2010 Elsevier B.V. All rights reserved.

  9. Profile analysis of microbiomes in soils of solonetz complex in the Caspian Lowland

    NASA Astrophysics Data System (ADS)

    Chernov, T. I.; Lebedeva, M. P.; Tkhakakhova, A. K.; Kutovaya, O. V.

    2017-01-01

    The taxonomic structure of the microbiota in two associated soils—solonetz on a microhigh and meadow-chestnut soil in a microlow—was studied in the semidesert of the Caspian Lowland. A highthroughput sequencing of the 16S rRNA gene was used for the soil samples from genetic horizons. A considerable reduction in the bacterial diversity was found in the lower horizons of the solonetz and compact solonetzic horizon with a high content of exchangeable sodium. In the meadow-chestnut soil, the microbial diversity little decreased with the depth. In both soils, a portion of archaea from the Thaumarchaeota group also decreased in the deeper horizons. In the soil horizons with the lower total bacterial diversity, a share of proteobacteria of the Enterobacteriaceae, Pseudomonadaceae, and Sphingomonadaceae families became higher. The difference between the structure of the microbial population in the solonetz and meadow- chestnut soil can be first explained by the different water regimes and soil consistence.

  10. Chemical composition and functional properties of native chestnut starch (Castanea sativa Mill).

    PubMed

    Cruz, Bruno R; Abraão, Ana S; Lemos, André M; Nunes, Fernando M

    2013-04-15

    Starch isolation methods can change their physico-chemical and functional characteristics hindering the establishment of a starch-food functionality relation. A simple high yield and soft isolation method was applied for chestnut (Castanea sativa Mill) starch consisting in steeping and fruit disintegration in a 25 mM sodium bisulfite solution and purification by sedimentation. Starch integrity, physico-chemical composition, morphology and functional properties were determined, being observed significant differences from previous described methods for chestnut starch isolation. The X-ray pattern was of B-type, with a degree of crystallinity ranging from 51% to 9%, dependent on the starch moisture content. The onset, peak, and conclusion gelatinization temperatures were 57.1°C, 61.9°C and 67.9°C, respectively. Total amylose content was 26.6%, and there was not found any evidence for lipid complexed amylose. Swelling power at 90°C was 19 g/g starch, and the amount of leached amylose was 78% of the total amylose content. Native chestnut starch presents a type B pasting profile similar to corn starch but with a lower gelatinization (56.1°C) and peak viscosity (79.5°C) temperatures, making native chestnut starch a potential technological alternative to corn starch, especially in application where lower processing temperatures are needed. Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. Anatolian honey is not only sweet but can also protect from breast cancer: Elixir for women from artemis to present.

    PubMed

    Seyhan, Mehmet Fatih; Yılmaz, Eren; Timirci-Kahraman, Özlem; Saygılı, Neslihan; Kısakesen, Halil İbrahim; Eronat, Allison Pınar; Ceviz, Ayşe Begüm; Bilgiç Gazioğlu, Sema; Yılmaz-Aydoğan, Hülya; Öztürk, Oğuz

    2017-09-01

    Natural products with bioactive components are widely studied on various cancer cell lines for their possible cytotoxic effects, recently. Among these products, honey stands out as a valuable bee product containing many active phenolic compounds and flavonoids. Numerous types of multifloral honey and honeydew honey are produced in Turkey owing to its abundant vegetation. Therefore, in this study, we investigated the cytotoxic effects of particular tree-originated honeys from chestnut, cedar, pine, and multifloral honey on cell lines representing different types of the most common cancer of women, breast cancer, MCF7, SKBR3, and MDAMB-231, and fibrocystic breast epithelial cell line, MCF10A as a control. All honey samples were analyzed biochemically. The dose- (1, 2.5, 5, 7.5, and 10 µg/mL) and time (24th, 48th, and 72nd hours)-dependent effects of ethanol/water solutions of the honey samples were scrutinized. Cell viability/cytotoxicity was evaluated by the water soluble tetrazolium Salt-1 (WST-1) method. Apoptotic status was detected by Annexin V-PI assay using FACSCalibur. The statistical analysis was performed using GraphPad Prism 6 and the clustering data analysis with the R programming language. The biochemical analyses of the honey samples showed that the tree-originated honey samples contained more total phenolic compounds than the multifloral honey. Phenolic content of the honey types increases in order of multifloral, pine, cedar, and chestnut, respectively, which is compatible with their cytotoxic affectivity and dark color. In addition, the antioxidant capacity of the studied honey types was observed to increase in order of multifloral < pine < cedar ≅ chestnut. According to the WST-1 data, chestnut honey induced cytotoxicity over 50% on all the cell lines, including the control MCF10A cells, even with low doses (honey concentrations starting from 1 µg/mL) (P < 0.0001). Similarly, Cedar honey was observed to be the second most effective honey in this study. Cedar honey, with the dose of 1 µg/mL, was detected statistically highly significant on MCF10A, MCF7, and SKBR3. In contrast, pine honey showed dramatically significant cytotoxicity only on the MDAMB 231 cells with a 1 µg/mL dose at the same time point (P = 0.018). While pine honey caused an anticancer effect on the MCF-7 and SKBR3 cancer cell lines with a 2.5-5 µg/mL dose (P < 0.0001), like cedar and chestnut honeys, it increased the viability of the MCF10A control cells with the doses of 2.5-5 µg/mL. It only showed cytotoxicity with higher doses (10 µg/mL) on the MCF10A cell line (P < 0.0001). Moreover, we have observed that the multifloral and artificial honey samples were mostly ineffective or increased cell viability with the doses of 1-5 µg/mL. Apoptotic effects of the other honey samples on the MCF-7 cell line were found as chestnut> pine> cedar> multifloral in the Annexin V-propidium iodide (PI) analysis. Chestnut, cedar, and pine honey displayed a remarkably cytotoxic effect on breast cancer cell lines, MCF7, SKBR3, and even on the most aggressive MDAMB 231, representing the triple negative breast cancer, which lacks of targeted anticancer therapy. The chestnut and cedar honeys stand out to be the most cytotoxic on all cell lines, while pine honey was found to be the least toxic on control cells with appropriate toxicity on the cancer cells. © 2017 IUBMB Life, 69(9):677-688, 2017. © 2017 International Union of Biochemistry and Molecular Biology.

  12. Testing resistance to chestnut blight of hybrid chestnuts

    Treesearch

    Cécile Robin; Xavier Capdevielle; Gille Saint-Jean; Teresa Barreneche

    2012-01-01

    Castanea sativa Mill. is an ecologically and economically important species in Europe, not only as a forest tree, but also as a fruit tree. It is dramatically threatened by ink disease caused by Phytophthora spp., introduced during the nineteenth century. To limit its impact, C. mollissima and C....

  13. U. S. Forest Service American Chestnut Cooperators' Meeting

    Treesearch

    Clay H. Smith

    1981-01-01

    On January 8 and 9, 1980, a 2-day meeting of U.S. Forest Service American chestnut cooperators was held at Pipestem State Park, Pipestem, West Virginia. A total of 43 talks were given a t this meeting. All speakers provided short abstracts of their talks; these abstracts are presented here.

  14. 78 FR 37842 - Notice of Availability of the Environmental Assessment and Notice of Public Hearing Federal Coal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-24

    ... marked chestnut, witnessed by a white oak, chestnut, and black gum marked as bearing trees, the 4th...'' E a distance of 6204.99 feet to a drilled hole in the top of an oval shaped rock on the east bank of...

  15. Mapping resistance to Phytophthora cinnamomi in chestnut (Castanea sp.)

    Treesearch

    Bode A. Olukolu; C. Dana Nelson; Albert G. Abbott

    2012-01-01

    Phytophthora cinnamomi (Phytophthora crown and root rot, or ink disease) is now known to infect several hundred plant species in the world and is especially linked to the widespread death of mature chestnut (Castanea) and evergreen oak (Quercus ilex L.) trees in southeast United States. With an expanding...

  16. Germplasm conservation for species restoration: Examples from efforts to restore the American chestnut

    Treesearch

    S.F. Fitzsimmons; K.M. Collins; J. Westbrook; T.M. Saielli; M.D. Brinckman

    2017-01-01

    American chestnut (Castanea dentata) was once a foundational species in much of its native range, especially in the Appalachian Mountains of the eastern United States. Unfortunately, the species was driven to functional extinction by the accidental importation of an exotic fungal pathogen (Cryphonectria parasitica), the causal...

  17. Testing American chestnuts for blight resistance

    Treesearch

    Jesse D. Diller

    1957-01-01

    It has now been over half a century since chestnut blight, caused by the fungus Endothia parasitica (Murr.) A. & A., was introduced into America from the Orient. In that time the blight has spread relentlessly and has destroyed all of our commercial stands of this once most valuable hardwood species of the East.

  18. Ecophysiology of horse chestnut (Aesculus Hippocastanum L.) in degraded and restored urban sites

    Treesearch

    Jacek Oleksyn; Brian D. Kloeppel; Szymon Lukasiewicz; Piotr Karolewski; Peter B. Reich

    2007-01-01

    We explored changes in growth, phenology, net CO2 assimilation rate, water use efficiency, secondary defense compounds, substrate and foliage nutrient concentration of a degraded urban horse chestnut (Aesculus hippocastanum L.) site restored for three years using mulching (tree branches including foliage) and fertilization (...

  19. Lithocarpus densiflorus (Hook. & Arn.) Rehd. tanoak

    Treesearch

    John C. Tappeiner; Philip M. McDonald; Douglass F. Roy

    1990-01-01

    Tanoak (Lithocarpus densiflorus), also called tanbark-oak, is an evergreen hardwood that, with other species in the genus, is considered a link between the chestnut, Castanea, and the oak, Quercus (19). Tanoak has flowers like the chestnut and acorns like the oak. This medium-sized tree grows best on the...

  20. Antioxidant and antimicrobial properties of Casteanea sativa Miller chestnut honey produced on Mount Etna (Sicily).

    PubMed

    Ronsisvalle, Simone; Lissandrello, Edmondo; Fuochi, Virginia; Petronio Petronio, Giulio; Straquadanio, Claudia; Crascì, Lucia; Panico, Annamaria; Milito, Marcella; Cova, Anna Maria; Tempera, Gianna; Furneri, Pio Maria

    2017-12-13

    The aim of this study was the evaluation of antibacterial and antioxidant properties of Monofloral Etna Castanea sativa Miller honeys. Escherichia coli ATCC 25,922, Pseudomonas aeruginosa ATCC 27,853, Enterococcus faecalis ATCC 29,211 and Staphylococcus aureus ATCC 29,213 were investigated for their susceptibilities to two different honeys. Antioxidant activity was evaluated by ORAC, NO scavenger assays, FRAP and DPPH. Antioxidant activity and antibacterial properties were compared with chestnut honeys from different geographical areas and with Manuka honey. UPLC-MS/MS was used for major components characterisation.

  1. Assessment of the effect of condensed (acacia and quebracho) and hydrolysable (chestnut and valonea) tannins on rumen fermentation and methane production in vitro.

    PubMed

    Hassanat, Fadi; Benchaar, Chaouki

    2013-01-01

    Tannins added to animal diets may have a positive effect on energy and protein utilisation in the rumen. The objective of this study was to examine the impact of different sources and concentrations (20, 50, 100, 150 and 200 g kg⁻¹ dry matter (DM)) of condensed (acacia and quebracho) and hydrolysable (chestnut and valonea) tannins on rumen microbial fermentation in vitro. The experiment also included a negative control with no tannins (control) and a positive control with monensin (10 mg L⁻¹). In vitro gas production and total volatile fatty acid (VFA) concentration decreased as tannin concentration increased. Addition of acacia, chestnut or valonea tannins at ≥ 50 g kg⁻¹ or quebracho tannins at ≥ 100 g kg⁻¹ resulted in a decrease (up to 40%) in methane (CH₄) production compared with the control. Valonea tannins were the only tannin source that reduced (-11%) CH₄ production at 50 g kg⁻¹ without affecting VFA concentration. Tannin treatments reduced ammonia (NH₃) and branched-chain VFA concentrations, indicating a reduction in ruminal protein degradation. Monensin reduced CH₄ production (-37%) and NH₃ concentration (-20%) without affecting total VFA concentration. Supplying acacia, chestnut or valonea tannins at 50 g kg⁻¹ has the potential to reduce CH₄ production and ruminal protein degradation with minimum detrimental effects on efficiency of ruminal fermentation. Copyright © 2012 Crown in the right of Canada. Published by John Wiley & Sons, Ltd.

  2. Changes in Cryphonectria parasitica Populations Affect Natural Biological Control of Chestnut Blight.

    PubMed

    Ježić, Marin; Mlinarec, Jelena; Vuković, Rosemary; Katanić, Zorana; Krstin, Ljiljana; Nuskern, Lucija; Poljak, Igor; Idžojtić, Marilena; Tkalec, Mirta; Ćurković-Perica, Mirna

    2018-07-01

    Invasive species, especially plant pathogens, have a potential to completely eradicate native plant species and remodel landscapes. Tripartite interactions among sweet chestnut (Castanea sativa), chestnut blight-causing invasive fungus Cryphonectria parasitica, and hyperparasitic virus Cryphonectria hypovirus 1 (CHV1) were studied in two populations. The number of different vegetative compatibility (vc) types of C. parasitica more than doubled over the 10 years, while the hypovirulence incidence dropped in one population and slightly increased in the other one. Over the course of our 3-year monitoring experiment, the prevalence of hypovirulent isolates obtained from monitored cankers increased slowly (i.e., more hypovirulent isolates were being obtained from the same cankers over time). Within studied cankers, considerable changes in vc type and CHV1 presence were observed, indicating a highly dynamic system in which virulent and hypovirulent mycelia, sometimes of discordant vc types, often appeared together. The increase in hypovirulence prevalence did not have any observable curative effect on the cankers and, occasionally, reactivation of healed cankers by new, virulent C. parasitica isolates was observed. Both short- and long-term observations and revalidation of the infected plant populations are necessary to accurately estimate disease progress and formulate an adequate disease management strategy.

  3. Antiviral escin derivatives from the seeds of Aesculus turbinata Blume (Japanese horse chestnut).

    PubMed

    Kim, Ji Won; Ha, Thi-Kim-Quy; Cho, Hyomoon; Kim, Eunhee; Shim, Sang Hee; Yang, Jun-Li; Oh, Won Keun

    2017-07-01

    Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and high fatality of piglets, influencing the swine industry. Japanese horse chestnut (seed of Aesculus turbinata) contains many saponin mixtures, called escins, and has been used for a long time as a traditional medicinal plant. Structure-activity relationship (SAR) studies on escins have revealed that acylations at C-21 and C-22 with angeloyl or tigloyl groups were important for their cytotoxic effects. However, the strong cytotoxicity of escins makes them hard to utilize for other diseases and to develop as nutraceuticals. In this research, we investigated whether escin derivatives 1-7 (including new compounds 2, 3, 5 and 6), without the angeloyl or tigloyl groups and with modified glycosidic linkages by hydrolysis, have PEDV inhibitory effects with less cytotoxicity. Compounds 1-7 had no cytotoxicity at 20μM on VERO cells, while compounds 8-10 showed strong cytotoxicity at similar concentrations on PEDV. Our results suggest that escin derivatives showed strong inhibitory activities on PEDV replication with lowered cytotoxicity. These studies propose a method to utilize Japanese horse chestnut for treating PEDV and to increase the diversity of its bioactive compounds. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Sensory profiling and consumer acceptability of new dark cocoa bars containing Tuscan autochthonous food products.

    PubMed

    Cantini, Claudio; Salusti, Patrizia; Romi, Marco; Francini, Alessandra; Sebastiani, Luca

    2018-03-01

    A new set of cocoa bars named Toscolata ® were developed containing top-quality extra virgin olive oil, dried apples cultivars, and chestnut flour. The present work has been conducted to define the sensory profile of these products through tasting by trained experts and consumers to study the acceptability, preference, and quality perception. The four sensorial profiles of the bars differed in the level of persistence, bitterness, aromaticity, acidity, astringency, and tastiness. In particular, the sour attribute could be traced to the presence of dried apple. Bars containing apple and chestnut flour obtained higher acceptance ratings, compared to those with extra virgin olive oil. The bar with chestnut flour was preferred by consumers who considered it to be sweeter due to the presence of natural sugars, which lowered the bitter sensation of cocoa. These results showed that the selection of the preferred bar by consumers was mainly based on the level of bitterness and, in particular, elderly consumers expressed a strong preference for the sweetest product. As far as we know, this is the first study comparing the results of a panel of expert tasters with that of consumers in the tasting of dark chocolate.

  5. Infectivity and sporulation of Phytophthora ramorum on northern red oak and chestnut oak

    USDA-ARS?s Scientific Manuscript database

    Branches from northern red and chestnut oak seedlings were dip-inoculated with 5,000 sporangia per milliliter of Phytophthora ramorum and incubated at 100 percent relative humidity in dew chambers for 6 days. Three plants were then used to assess sporangia production, while the other three plants w...

  6. Coumarins in horse chestnut flowers: isolation and quantification by UPLC method.

    PubMed

    Dudek-Makuch, Marlena; Matławska, Irena

    2013-01-01

    The coumarins: scopoletin, esculetin and fraxetin were isolated from the flowers of horse chestnut (Aesculus hippocastanum L., Hippocastanaceae) and identified by spectrophotometric methods (UV, 1H, 13C NMR, ESI-MS). Their content, determined using the Ultra Performance Liquid Chromatography (UPLC), was 0.41, 0.13 and 0.05%, respectively.

  7. Resistance to Phytophthora cinnamomi among seedlings from backcross families of hybrid american chestnut

    Treesearch

    Steven N. Jeffers; Inga M. Meadows; Joseph B. James; Paul H. Sisco

    2012-01-01

    American chestnut (Castanea dentata (Marsh.) Borkh.) once was a primary hardwood species in forests of the eastern United States. Sometime during the late 18th century, it is speculated that Phytophthora cinnamomi, which causes Phytophthora root rot (PRR) on many woody plant species, was introduced to the southeast region of...

  8. Genetic structure of American chestnut populations based on neutral DNA markers

    Treesearch

    Thomas L. Kubisiak; James H. Roberds

    2006-01-01

    Microsatellite and RAPD markers suggest that American chestnut exists as a highly variable species. Even at the margins of its natural range, with a large proportion of its genetic variability occurring within populations (~95%). A statistically significant proportion also exists among population. Although genetic differentiation among populations has taken place, no...

  9. Antioxidant potential of chestnut (Castanea sativa L.) and almond (Prunus dulcis L.) by-products.

    PubMed

    Barreira, J C M; Ferreira, I C F R; Oliveira, M B P P; Pereira, J A

    2010-06-01

    The antioxidant properties of almond green husks (Cvs. Duro Italiano, Ferraduel, Ferranhês, Ferrastar and Orelha de Mula), chestnut skins and chestnut leaves (Cvs. Aveleira, Boa Ventura, Judia and Longal) were evaluated through several chemical and biochemical assays in order to provide a novel strategy to stimulate the application of waste products as new suppliers of useful bioactive compounds, namely antioxidants. All the assayed by-products revealed good antioxidant properties, with very low EC(50) values (lower than 380 μg/mL), particularly for lipid peroxidation inhibition (lower than 140 μg/mL). The total phenols and flavonoids contents were also determined. The correlation between these bioactive compounds and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation in pig brain tissue through formation of thiobarbituric acid reactive substances, was also obtained. Although, all the assayed by-products proved to have a high potential of application in new antioxidants formulations, chestnut skins and leaves demonstrated better results.

  10. Phylogeography of Japanese horse chestnut (Aesculus turbinata) in the Japanese Archipelago based on chloroplast DNA haplotypes.

    PubMed

    Sugahara, Kanako; Kaneko, Yuko; Ito, Satoshi; Yamanaka, Keisuke; Sakio, Hitoshi; Hoshizaki, Kazuhiko; Suzuki, Wajiro; Yamanaka, Norikazu; Setoguchi, Hiroaki

    2011-01-01

    Japanese horse chestnut (Aesculus turbinata: Hippocastanaceae) is one of the typical woody plants that grow in temperate riparian forests in the Japanese Archipelago. To analyze the phylogeography of this plant in the Japanese Archipelago, we determined cpDNA haplotypes for 337 samples from 55 populations covering the entire distribution range. Based on 1,313 bp of two spacers, we determined ten haplotypes that are distinguished from adjacent haplotypes by one or two steps. Most of the populations had a single haplotype, suggesting low diversity. Spatial analysis of molecular variance suggested three obvious phylogeographic structures in western Japan, where Japanese horse chestnut is scattered and isolated in mountainous areas. Conversely, no clear phylogeographic structure was observed from the northern to the southern limit of this species, including eastern Japan, where this plant is more common. Rare and private haplotypes were also found in southwestern Japan, where Japanese horse chestnuts are distributed sparsely. These findings imply that western Japan might have maintained a relatively large habitat for A. turbinata during the Quaternary climatic oscillations, while northerly regions could not.

  11. Chestnut astringent skin extract, an alpha-amylase inhibitor, retards carbohydrate absorption in rats and humans.

    PubMed

    Tsujita, Takahiro; Takaku, Takeshi; Suzuki, Tsuneo

    2008-02-01

    Inhibitors of carbohydrate-hydrolyzing enzyme play an important role to control postprandial blood glucose levels. In this paper, we investigated the effect of an ethanol extract from chestnut astringent skin (CAS) on alpha-amylase. Chestnut astringent skin extract strongly inhibited human and porcine pancreatic alpha-amylase. We also investigated the effect of CAS extract on carbohydrate absorption in rats and humans. Oral administration of CAS extract to normal rats fed corn starch (2 g/kg body weight), significantly suppressed the increase of blood glucose levels after starch loading in a dose-dependent manner. The effective dose of CAS extract required to achieve 20 and 40% suppression of the rise in blood glucose level was estimated to be 40 and 155 mg/kg body weight, respectively. Chestnut astringent skin extract also suppressed the rise in plasma insulin level and the fall in plasma non-esterified fatty acid level. In the type 2 diabetic rat model, CAS extract significantly suppressed the rise in blood glucose level after starch loading in a dose-dependent manner. Chestnut astringent skin extract also suppressed the rise in plasma glucose level after boiled rice loading in a dose-dependent manner in humans. The amount of CAS extract required to achieve 11 and 23% suppression in the rise in plasma glucose level was 300 and 600 mg/person, respectively. These results suggest that CAS extract retards absorption of carbohydrate and reduces post-prandial hyperglycemia.

  12. Estimating the ecology of extinct species with paleoecological data assimilation

    NASA Astrophysics Data System (ADS)

    Raiho, A.; McLachlan, J. S.; Dietze, M.

    2017-12-01

    In order to understand long term, unobservable ecosystem processes, ecologists must use both paleoecoloigcal data and ecosystem models. Models parameterize species competitive interactions using modern data. But, modern ecological or physiological observations are not available for extinct species, making it difficult for models to conceptualize their ecology. For instance, American chestnut (Castanea dentata), who played a large role in forests of northeastern US, was decimated by disease to virtual extinction. Since chestnut's demise, defining its ecology has been controversial. Models typically assume that chestnut's ecology was very similar to oak; They parameterize chestnut like oak species. These assumptions are drawn from paleoecological data, but these data are often reported without uncertainty. Since the paleoecological data are often reported without uncertainty, paleoecological data has never been directly incorporated with ecosystem models. We developed a Bayesian statistical model to estimate fractional composition from paleoecological data with uncertainty. Then, we assimilated this data product into an ecosystem model for long term forest succession using a generalized ensemble adjustment filter to determine which species demographic parameters lead to changes in species composition over the last 2,000 years at Harvard Forest. We found that chestnut was strongly negatively correlated with white pine (Pinus strobus) and red oak (Quercus rubra) in the process covariance matrix, suggesting a strong competitive interaction that is not currently understood by models for forest succession. These findings provide support for utilizing a data assimilation framework to ecologically interpret paleoecological data or data products to learn about the ecology of extinct species.

  13. Authentication of the botanical origin of honey by front-face fluorescence spectroscopy. A preliminary study.

    PubMed

    Ruoff, Kaspar; Karoui, Romdhane; Dufour, Eric; Luginbühl, Werner; Bosset, Jacques-Olivier; Bogdanov, Stefan; Amado, Renato

    2005-03-09

    The potential of front-face fluorescence spectroscopy for the authentication of unifloral and polyfloral honey types (n = 57 samples) previously classified using traditional methods such as chemical, pollen, and sensory analysis was evaluated. Emission spectra were recorded between 280 and 480 nm (excit: 250 nm), 305 and 500 nm (excit: 290 nm), and 380 and 600 nm (excit: 373 nm) directly on honey samples. In addition, excitation spectra (290-440 nm) were recorded with the emission measured at 450 nm. A total of four different spectral data sets were considered for data analysis. After normalization of the spectra, chemometric evaluation of the spectral data was carried out using principal component analysis (PCA) and linear discriminant analysis (LDA). The rate of correct classification ranged from 36% to 100% by using single spectral data sets (250, 290, 373, 450 nm) and from 73% to 100% by combining these four data sets. For alpine polyfloral honey and the unifloral varieties investigated (acacia, alpine rose, honeydew, chestnut, and rape), correct classification ranged from 96% to 100%. This preliminary study indicates that front-face fluorescence spectroscopy is a promising technique for the authentication of the botanical origin of honey. It is nondestructive, rapid, easy to use, and inexpensive. The use of additional excitation wavelengths between 320 and 440 nm could increase the correct classification of the less characteristic fluorescent varieties.

  14. Decomposition rates of American chestnut (Castanea dentata) wood and implications for coarse woody debris pools

    Treesearch

    Arjan de Bruijn; Eric J. Gustafson; Daniel M. Kashian; Harmony J. Dalgleish; Brian R. Sturtevant; Douglass F. Jacobs

    2014-01-01

    Observations of the rapid growth and slow decomposition of American chestnut (Castanea dentata (Marsh.) Borkh.) suggest that its reintroduction could enhance terrestrial carbon (C) sequestration. A suite of decomposition models was fit with decomposition data from coarse woody debris (CWD) sampled in Wisconsin and Virginia, U.S. The optimal (two-...

  15. The chapter breeding program of the American Chestnut Foundation

    Treesearch

    Sara Fitzsimmons; Kendra Gurney; William White; Katy McCune

    2012-01-01

    A unique feature of the American Chestnut Foundation breeding program is the use of volunteers to conduct most of the regional breeding that will help increase genetic diversity and preserve local adaptation in the products of our program. This effort is coordinated by the four authors of this abstract, who are employees of the Foundation. The Foundation has...

  16. Sporulation capacity of Phytophthora ramorum on northern red oak and chestnut oak

    USDA-ARS?s Scientific Manuscript database

    Branches from six 2 to 3-year old northern red and chestnut oak seedlings were dip-inoculated with ca. 5,000 sporangia per milliliter of Phytophthora ramorum isolate Pr-6 and incubated at 100 percent relative humidity in dew chambers for 6 days. Three plants were then used to assess sporangia produ...

  17. Genetic Variation in Natural Populations of American Chestnut

    Treesearch

    Thomas L. Kubisiak; James H. Roberds

    2003-01-01

    Prior to the blight epidemic, American chestnut (Castanea dentata Borkh.) was one of the most important timber and nut-producing tree species in eastern North America (U.S. Census Bureau 1908). Its native range extended from southern Maine and Ontario in the north to Georgia, Alabama and Mississippi in the south (Sargent 1905). It now exists...

  18. Exotic pests of eastern forests conference proceedings

    Treesearch

    Kerry O. Britton

    1998-01-01

    Invasive exotic pest plants, diseases, and insects, have had a dramatic impact on the health and composition of the Eastern forests for many decades. Chestnut blight was discovered in the United States in 1904. Since then, it has virtually destroyed the chestnut population, which once occupied 25 percent of the eastern forest. In the 1860's, the gypsy moth was...

  19. Response of chestnut oak and red oak to drought and fertilization: growth and physiology

    Treesearch

    M.D. Kleiner; M.D. Abrams; J.C. Schultz

    1991-01-01

    Chestnut oak (Quercus prinus L.) and red oak (Quercus rubra L.) seedlings were grown for two seasons under two nutrient regimes: fertilizer + (NPK) and fertilizer - (No NPK). Beginning two weeks after budbreak, water was withheld for 10 weeks during the second growing season. Leaf water potentials, gas exchange measurements and...

  20. Allozyme and RAPD Analysis of the Genetic Diversity and Geographic Variation in Wild Populations of the American Chestnut (Fagaceae)

    Treesearch

    Hongwen Huang; Fenny Dane; Thomas L. Kubisiak

    1998-01-01

    Genetic variation among 12 populations of the American chestnut (Custanea dentata) was investigated. Population genetic parameters estimated from allozyme variation suggest that C. dentata at both the population and species level has narrow genetic diversity as compared to other species in the genus. Average expected heterozygosity...

  1. Do chestnut, northern red, and white oak germinant seedlings respond similary to light treatments? II. Gas exchange and chlorophyll responses

    Treesearch

    Joanne Rebbeck; Amy Scherzer; Kurt Gottschalk

    2012-01-01

    Understanding differences in physiological and growth strategies in low-light environments among upland oak species may help managers address the challenges of oaks' poor regeneration. Gas exchange and chlorophyll content were measured for northern red oak (Quercus rubra L.), chestnut oak (Quercus prinus L.), and white oak (...

  2. A case of gastric perforation caused by chestnut bezoars.

    PubMed

    Okagawa, Yutaka; Takada, Kohichi; Arihara, Yohei; Kato, Junji

    A 65-year-old man was admitted under emergency to our hospital because of abdominal pain. His current medication history did not include steroids or nonsteroidal antiinflammatory drugs. He had taken an eradication agent for Helicobacter pylori, and his serum was negative for H. pylori IgG antibody. Abdominal computed tomography indicated gastric perforation;therefore, emergency surgery was performed. Two weeks later, esophagogastroduodenoscopy revealed a gastric ulcer on the lesser curvature of the gastric angle and bezoars. The gastric perforation was thought to be caused by the bezoars. The bezoars were successfully treated with endoscopic therapy using Coca-Cola ® . The bezoars included over 98% tannin, and the patient had frequently consumed chestnuts. We thus diagnosed a rare case of gastric perforation caused by chestnut bezoars.

  3. Effects of feed-supplementation and hide-spray application of two sources of tannins on enteric and hide bacteria of feedlot cattle.

    PubMed

    Gutierrez-Banuelos, Hector; Pinchak, William E; Min, Byeng R; Carstens, Gordon E; Anderson, Robin C; Tedeschi, Luis O; Krueger, Wimberley K; Krueger, Nathan A; Lancaster, Phillip A; Gomez, Robynne R

    2011-01-01

    Pathogenic bacteria attached to the hide or shed in the feces of cattle at slaughter can contaminate carcasses intended to be processed for human consumption. Therefore, new pre-harvest interventions are needed to prevent the carriage and excretion of foodborne pathogens in cattle presented to the processing plant. The objectives of this study were to examine the antimicrobial effects of hydrolysable tannin-rich chestnut and condensed tannin-rich mimosa extracts on bacterial indicators of foodborne pathogens when applied as a hide-intervention and as a feed additive to feedlot cattle. Water (control) or solutions (3 % wt/vol) of chestnut- and mimosa-extract treatments were sprayed (25 mL) at the left costal side of each animal to a 1000 cm² area, divided in four equal quadrants. Hide-swabs samples obtained at pre-, 2-min, 8-h, and 24-h post-spray application were cultured to enumerate Escherichia coli/total coliforms and total aerobic plate counts. In a second experiment, diets supplemented without (controls) or with (1.5 % of diet dry matter) chestnut- or mimosa-extracts were fed during a 42-day experimental feeding period. Weekly fecal samples starting on day 0, and rumen fluid obtained on days 0, 7, 21 or 42 were cultured to enumerate E.coli/total coliforms and Campylobacter. Tannin spray application showed no effect of treatment or post-application-time (P > 0.05) on measured bacterial populations, averaging 1.7/1.8, 1.5/1.6 and 1.5/1.7 (log₁₀CFU/cm²) for E. coli/total coliforms, and 4.0, 3.4 and 4.2 (log₁₀CFU/cm²) in total aerobes for control, chestnut and mimosa treatments, respectively. Mean (± SEM) ruminal E. coli and total coliform concentrations (log(10) CFU/mL) were reduced (P < 0.01) in steers fed chestnut-tannins (3.6 and 3.8 ± 0.1) in comparison with the controls (4.1 and 4.2 ± 0.1). Fecal E. coli concentrations were affected by treatment (P< 0.01), showing the highest values (log₁₀ CFU/g) in fecal contents from mimosa-fed steers compared to controls (5.9 versus 5.6 ± 0.1 SEM, respectively). Total coliforms (log CFU/g) showed the highest values (P < 0.01) in feces from chestnut- and mimosa-fed steers (6.0 and 6.1 ± 0.1 respectively) in comparison with controls (5.7 ± 0.1). Fecal Campylobacter concentrations (log₁₀CFU/g) were affected by treatment (P < 0.05), day (P < 0.001) and their interaction (P < 0.01) with the controls having lower concentrations than chestnut- and mimosa-fed steers (0.4, 1.0, and 0.8 ± 0.3, respectively). It was concluded that under our research conditions, tannins were not effective in decreasing measured bacterial populations on beef cattle hides. Additionally, chestnut tannin reduced E. coli and total coliforms within the rumen but the antimicrobial effect was not maintained in the lower gastrointestinal tract. Further research is necessary to elucidate the possible antimicrobial effects of tannins at site-specific locations of the gastrointestinal tract in beef cattle fed high-grain and high-forage diets.

  4. Biological object recognition in μ-radiography images

    NASA Astrophysics Data System (ADS)

    Prochazka, A.; Dammer, J.; Weyda, F.; Sopko, V.; Benes, J.; Zeman, J.; Jandejsek, I.

    2015-03-01

    This study presents an applicability of real-time microradiography to biological objects, namely to horse chestnut leafminer, Cameraria ohridella (Insecta: Lepidoptera, Gracillariidae) and following image processing focusing on image segmentation and object recognition. The microradiography of insects (such as horse chestnut leafminer) provides a non-invasive imaging that leaves the organisms alive. The imaging requires a high spatial resolution (micrometer scale) radiographic system. Our radiographic system consists of a micro-focus X-ray tube and two types of detectors. The first is a charge integrating detector (Hamamatsu flat panel), the second is a pixel semiconductor detector (Medipix2 detector). The latter allows detection of single quantum photon of ionizing radiation. We obtained numerous horse chestnuts leafminer pupae in several microradiography images easy recognizable in automatic mode using the image processing methods. We implemented an algorithm that is able to count a number of dead and alive pupae in images. The algorithm was based on two methods: 1) noise reduction using mathematical morphology filters, 2) Canny edge detection. The accuracy of the algorithm is higher for the Medipix2 (average recall for detection of alive pupae =0.99, average recall for detection of dead pupae =0.83), than for the flat panel (average recall for detection of alive pupae =0.99, average recall for detection of dead pupae =0.77). Therefore, we conclude that Medipix2 has lower noise and better displays contours (edges) of biological objects. Our method allows automatic selection and calculation of dead and alive chestnut leafminer pupae. It leads to faster monitoring of the population of one of the world's important insect pest.

  5. Impact of fertilization on chestnut growth, N and P concentrations in runoff water on degraded slope land in South China.

    PubMed

    Zeng, Shu-Cai; Chen, Bei-Guang; Jiang, Cheng-Ai; Wu, Qi-Tang

    2007-01-01

    Growing fruit trees on the slopes of rolling hills in South China was causing serious environmental problems because of heavy application of chemical fertilizers and soil erosion. Suitable sources of fertilizers and proper rates of applications were of key importance to both crop yields and environmental protection. In this article, the impact of four fertilizers, i.e., inorganic compound fertilizer, organic compound fertilizer, pig manure compost, and peanut cake (peanut oil pressing residue), on chestnut (Castanea mollissima Blume) growth on a slope in South China, and on the total N and total P concentrations in runoff waters have been investigated during two years of study, with an orthogonal experimental design. Results show that the organic compound fertilizer and peanut cake promote the heights of young chestnut trees compared to the control. In addition, peanut cake increases single-fruit weights and organic compound fertilizer raises single-seed weights. All the fertilizers increased the concentrations of total N and total P in runoff waters, except for organic compound fertilizer, in the first year experiment. The observed mean concentrations of total N varied from 1.6 mg/L to 3.2 mg/L and P from 0.12 mg/L to 0.22 mg/L, which were increased with the amount of fertilizer applications, with no pattern of direct proportion. On the basis of these experiment results, organic compound fertilizer at 2 kg/tree and peanut cake at 1 kg/tree are recommended to maximize chestnut growth and minimize water pollution.

  6. Efficacy of washing treatments in the reduction of postharvest decay of chestnuts (Castanea crenata 'Tsukuba') during storage

    Treesearch

    Uk Lee; Sukhyun Joo; Ned B. Klopfenstein; Mee-Sook Kim

    2016-01-01

    This research evaluated the influence of different washing treatments (i.e., tap water, ozone, microbubbles, and ozone combined with microbubbles) on post-harvest decay of chestnuts (Castanea crenata ‘Tsukuba’) during storage. Overall, treatments with ozone and microbubbles significantly reduced the decay frequency and the associated microbial populations (...

  7. Native mycorrhizal fungi replace introduced fungal species on Virginia pine and American chestnut planted on reclaimed mine sites of Ohio

    Treesearch

    Shivanand Hiremath; Kirsten Lehtoma; Jenise M. Bauman

    2014-01-01

    Plant-microbe community dynamics influence the natural succession of plant species where pioneer vegetation facilitates the establishment of a distantly related, later successional plant species. This has been observed in the case of restoration of the American chestnut (Castanea dentata) on abandoned mine land where Virginia pine (Pinus...

  8. American chestnut restoration in New England - cold damage as an added challenge

    Treesearch

    Paul Schaberg; Paula Murakami; Gary J. Hawley; Kendra Collins

    2017-01-01

    The American chestnut (Castanea dentata) was once an ecological and economic keystone species in the eastern United States, and once comprised up to 50% of the basal area in portions of the Appalachian hardwood forest (Braun 1950). Its stature was impressive (some over 120 feet tall) and it grew remarkably fast (up to an inch in diameter per year...

  9. Consequences of shifts in abundance and distribution of American chestnut for restoration of a foundation forest tree

    Treesearch

    Harmony Dalgleish; C. Dana Nelson; John Scrivani; Douglass Jacobs

    2015-01-01

    Restoration of foundation species, such as the American chestnut (Castanea dentata) that was devastated by an introduced fungus, can restore ecosystem function. Understanding both the current distribution as well as biogeographic patterns is important for restoration planning. We used United States Department of Agriculture Forest...

  10. Production comparisons of Chinese water chestnut [Eleocharis dulcis (Burm. f.) Trin. ex Hensch] functional corms grown in hydroponics versus flooded sand

    USDA-ARS?s Scientific Manuscript database

    Chinese water chestnut [Eleocharis dulcis (Burm. f.) Trin. ex Hensch.] corms are used as a canned or raw vegetable worldwide and may have potential use as a functional vegetable for human health uses. The accessions in the USDA, ARS, Plant Genetic Resources Conservation Unit do not produce very many...

  11. Screening for Phytophthora cinnamomi in reclaimed mined lands targeted for American chestnut restoration projects

    Treesearch

    Shiv Hiremath; Kirsten Lehtoma; Annemarie Nagle; Pierluigi Bonello

    2011-01-01

    We are working toward restoring the American chestnut in southeastern Ohio, which was once part of the tree's natural range. Some of these lands have been severely affected by excessive mining operations for several decades. Therefore, we are planning and testing use of ectomycorrhizal fungi in the restoration efforts. Mycorrhizal fungi may play a vital role in...

  12. Incidence of twolined chestnut borer and Hypoxylon atropunctatum on dead oaks along an acidic deposition gradient from Arkansas to Ohio

    Treesearch

    R.A. Haack; R.W. Blank

    1991-01-01

    The incidence of twolined chestnut borer (TLCB), Agrilus bilineatus (Weber), and the canker fungus Hypoxylon atropunctatum (Schw. ex Fr.) Cke. was recoreded on dead oak (Quercus) trees !Y7 cm diameter at breast height (DBH) along an acidic deposition gradient from Arkansas to Ohio in 1989 and 1990. Approximately...

  13. Complex of solonetzes and vertic chestnut soils in the manych-gudilo depression

    NASA Astrophysics Data System (ADS)

    Kovda, I. V.; Morgun, E. P.; Il'ina, L. P.

    2013-01-01

    Morphological, physicochemical, and isotopic properties of a two-member soil complex developed under dry steppe have been studied in the central part of the Manych Depression. The soils are formed on chocolate-colored clayey sediments, and have pronounced microrelief and the complex vegetation pattern. A specific feature of the studied soil complex is the inverse position of its components: vertic chestnut soil occupies the microhigh, while solonetz is in the microlow. The formation of such complexes is explained by the biological factor, i.e., by the destruction of the solonetzic horizon under the impact of vegetation and earth-burrowing animals with further transformation under steppe plants and dealkalinization of the soil in the microhighs. The manifestation of vertic features and shrink-swell process in soils of the complex developing in dry steppe are compared with those in the vertic soils of the Central Pre-Caucasus formed under more humid environment. It is supposed that slickensides in the investigated vertic chestnut soil are relict feature inherited from the former wetter stage of the soil development and are subjected to a gradual degradation at present. In the modern period, vertic processes are weak and cannot be distinctly diagnosed. However, their activation may take place upon an increase of precipitation or the rise in the groundwater level.

  14. Antioxidative and antigenotoxic effects of Japanese horse chestnut (Aesculus turbinata) seeds.

    PubMed

    Sato, Itaru; Suzuki, Tadahiko; Kobayashi, Haruo; Tsuda, Shuji

    2005-07-01

    Japanese horse chestnut seed extract (HCSE) dose-dependently inhibited the autooxidation of linoleic acid (IC(50): 0.2 mg/ml), and the inhibition was almost complete at a concentration of 1 mg/ml. The HCSE scavenged DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals and superoxide anions with EC(50)s of 0.65 and 0.21 mg/ml, respectively. However, it had no effect on hydrogen peroxide. The HCSE inhibited the genotoxicities of furylfuramide, N-methyl-N-nitrosourea, methyl methanesulfonate, mitomycin C, 2-aminoanthracene and aflatoxin B1 at a concentration of 1 mg/ml or more. Total polyphenol content of the HCSE was 21 mg/g (13 mg/g-seeds). These results indicate that the Japanese horse chestnut seed is an antioxidative and antimutagenic botanical resource.

  15. [Control of Soil Nutrient Loss of Typical Reforestation Patterns Along the Three Gorges Reservoir Area].

    PubMed

    Wu, Dong; Huang, Zhi-lin; Xiao, Wen-fa; Zeng, Li-xiong

    2015-10-01

    Annual soil nutrient loss characteristics on typical reforestation patterns in watershed along the Three Gorges Reservoir Area were studied based on runoff plot experiment. Runoff and sediment nutrition content from May to October 2014 of typical reforestation patterns including garden plot (tea garden), forest land (Chinese chestnut) and the original slope farmland were determined and then analyzed. The results showed that: (1) After the Returning Farmland to Forest Project the quantity of annual soil nutrient (nitrogen and phosphorus, the sum of them in sediment and runoff) loss decreased. The output of total nitrogen (TN) was in the order of slope farmland (2 444.27 g x hm(-2)) > tea garden (998.70 g x hm(-2)) > Chinese chestnut forest (532.61 g x hm(-2)), and for total phosphorus (TP) loss was slope farmland (1 690.48 g x hm(-2)) > tea garden (488.06 g x hm(-2)) > Chinese chestnut forest (129.00 g x hm(-2)) . Compared with slope farmland, the load of TN and TP output of reforestation patterns decreased 68.68% and 81.75%, respectively. (2) Compared with slope farmland, available nitrogen loss decreased in reforestation patterns. Total nitrate nitrogen (NO3(-)-N) loss ranked in the order of slope farmland (113.79 g x hm(-2)) > tea garden (73.75 g x hm(-2)) > Chinese chestnut forest (56.06 g x hm(-2)) The largest amount of ammonium nitrogen (NH4(+)-N) was found in tea garden (69.34 g x hm(-2)), then in farmland (52.45 g x hm(-2)), and the least in Chinese chestnut forest (47.23 g x hm(-2)). (3) The main route of NO3(-)-N and NH4(+)-N loss was both through runoff, the quantity of NO3(-)-N and NH4(+)-N output in which accounted for 91.4% and 92.2% of the total, respectively. The quantity of TN and TP in sediment accounted for 86.6% and 98.4% of the total. TN and TP loss showed an extremely significant correlation with sediments, which showed that sediment output was the main approach of TN and TP loss.

  16. Deposition pattern and throughfall fluxes in secondary cool temperate forest, South Korea

    NASA Astrophysics Data System (ADS)

    Kumar Gautam, Mukesh; Lee, Kwang-Sik; Song, Byeong-Yeol

    2017-07-01

    Chemistry and deposition fluxes in the rainfall and throughfall of red pine (Pinus densiflora), black locust (Robinia pseudoacacia), and chestnut (Castanea crenata) monocultures, and mixed red pine-black locust-chestnut stands were examined in a nutrient-limited cool temperate forest of central South Korea. Throughfall was enriched in both basic and acidic constituents relative to rainfall, suggesting that both dry deposition and canopy leaching are important sources of throughfall constituents. Net throughfall fluxes (NTFs) of cations and anions significantly differed among four different stands as well as seasonally. Red pine exhibited highest fluxes (TF and NTF) for Ca2+, black locust for K+, mixed stands for Mg2+, and chestnut for Na+. In contrast, NTF of SO42-, NO3-, and NH4+was highest in the red pine, intermediate in the chestnut and mixed stands, and lowest in the black locust. In general, canopy uptake of H+ and NH4+ for all stands was higher in summer than in winter. Dry deposition appears to play a major role in atmospheric deposition to this cool temperate forest, especially in summer. Dry deposition for both cations and anions displayed high spatial variability, even though stands were adjacent to one another and experienced identical atmospheric deposition loads. Canopy leaching of K+ (95-78% of NTF), Mg2+ (92-23% of NTF), and Ca2+ (91-12% of NTF) was highest for the black locust, lowest for chestnut, and intermediate for the red pine and mixed stands. The present study documented significant changes in throughfall chemistry and NTF among different forest stands, which presumably be related with the differences in the canopy characteristics and differences in their scavenging capacity for dry deposition and canopy exchange. Difference in the canopy retention of H+ and base cation leaching suggests that canopy exchange was mainly driven by weak acid excretion and lesser by H+ exchange reaction. Our results indicate that despite a high base cation deposition, a combination of higher input of acidifying constituents, low soil pH, and total acidic deposition approaching South Korean critical loads make regional forest vulnerable to acidification.

  17. Castanea sativa (European Chestnut) Leaf Extracts Rich in Ursene and Oleanene Derivatives Block Staphylococcus aureus Virulence and Pathogenesis without Detectable Resistance

    PubMed Central

    Quave, Cassandra L.; Lyles, James T.; Kavanaugh, Jeffery S.; Nelson, Kate; Parlet, Corey P.; Crosby, Heidi A.; Heilmann, Kristopher P.; Horswill, Alexander R.

    2015-01-01

    The Mediterranean is home to a rich history of medical traditions that have developed under the influence of diverse cultures over millennia. Today, many such traditions are still alive in the folk medical practices of local people. Investigation of botanical folk medicines used in the treatment of skin and soft tissue infections led us to study Castanea sativa (European Chestnut) for its potential antibacterial activity. Here, we report the quorum sensing inhibitory activity of refined and chemically characterized European Chestnut leaf extracts, rich in oleanene and ursene derivatives (pentacyclic triterpenes), against all Staphylococcus aureus accessory gene regulator (agr) alleles. We present layers of evidence of agr blocking activity (IC50 1.56–25 μg mL-1), as measured in toxin outputs, reporter assays hemolytic activity, cytotoxicity studies, and an in vivo abscess model. We demonstrate the extract’s lack of cytotoxicity to human keratinocytes and murine skin, as well as lack of growth inhibitory activity against S. aureus and a panel of skin commensals. Lastly, we demonstrate that serial passaging of the extract does not result in acquisition of resistance to the quorum quenching composition. In conclusion, through disruption of quorum sensing in the absence of growth inhibition, this study provides insight into the role that non-biocide inhibitors of virulence may play in future antibiotic therapies. PMID:26295163

  18. Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs

    Treesearch

    D.A. ​Varley; G.K. Podila; S.T. Hiremath

    1992-01-01

    Plant-pathogenic fungi produce cutinase, an enzyme required to degrade plant cuticles and facilitate penetration into the host. The absence of cutinase or a decrease in its production has been associated with a decrease in pathogenicity of the fungus. A set of isogenic strains of Cryphonectria parasitica, the chestnut blight fungus, was tested for...

  19. Long-term changes in forest composition and diversity following early logging (1919-1923) and the decline of American chestnut (Castanea dentata)

    Treesearch

    Katherine J. Elliott; Wayne T. Swank

    2008-01-01

    Chestnut blight fungus (Endothia parasitica [Murr.] P.I. And. & H.W. And. is a classic example of an invasive species, which severely damaged populations of its host, Castanea dentata, and had widespread and long-term impacts on eastern North American forests. Concurrently, forests were further disturbed by lumbering, which was common across the...

  20. Effects of polyphenols from seed shells of Japanese horse chestnut (Aesculus turbinata BLUME) on methotrexate-induced intestinal injury in rats.

    PubMed

    Sugiyama, Akihiko; Kimura, Hideto; Ogawa, Satoshi; Yokota, Kazushige; Takeuchi, Takashi

    2011-05-01

    The purpose of this study was to evaluate the effects of polyphenols from seed shells of Japanese horse chestnut (JHP) on methotrexate (MTX)-induced intestinal injury in rats. MTX application caused intestinal morphological injury and increase in malondialdehyde (MDA) levels, decrease in levels of glutathione (GSH) and glutathione peroxidase (GSH-Px) activities in small intestine. However, oral administration of JHP ameliorated MTX-induced intestinal injury and inhibited the increase in MDA and the decrease in GSH and GSH-Px activity in small intestine. In conclusion, our results indicated that oral administration of JHP alleviated MTX-induced intestinal injury through its antioxidant properties.

  1. Biogas production from plant biomass used for phytoremediation of industrial wastes.

    PubMed

    Verma, V K; Singh, Y P; Rai, J P N

    2007-05-01

    In present study, potentials of water hyacinth (Eichhornia crassipes) and water chestnut (Trapa bispinnosa) employed for phytoremediation of toxic metal rich brass and electroplating industry effluent, were examined in terms of biogas generation. Inability of the plants to grow in undiluted effluent directed to select 20%, 40% and 60% effluent concentrations (with deionized water) for phytoremediation experiments. Slurry of both the plants used for phytoremediation produced significantly more biogas than that by the control plants grown in unpolluted water; the effect being more pronounced with plants used for phytoremediation of 20% effluent. Maximum cumulative production of biogas (2430c.c./100gdm of water hyacinth and 1940c.c./100gdm of water chest nut) and per cent methane content (63.82% for water hyacinth and 57.04% for water chestnut) was observed at 5mm particle size and 1:1 substrate/inoculum ratio, after twenty days incubation. Biogas production was quicker (maximum from 8-12days) in water hyacinth than in water chestnut (maximum from 12-16days). The qualitative and quantitative variations in biogas production were correlated with COD, C, N, C/N ratio and toxic metal contents of the slurry used.

  2. Yeast Communities of Chestnut Soils under Vineyards in Dagestan

    NASA Astrophysics Data System (ADS)

    Abdullabekova, D. A.; Magomedova, E. S.; Magomedov, G. G.; Aliverdieva, D. A.; Kachalkin, A. V.

    2017-12-01

    The study of yeast communities in chestnut soils (Kastanozems) under vineyards in the Republic of Dagestan made it possible to isolate 20 yeast species. Most of the yeasts under vineyards belonged to ascomycetes, among which species of the Saccharomycetaceae family (in particular, Saccharomyces cerevisiae) comprised a significant part. The obtained results indicate that the soils under vineyards keep the pool of microbial diversity and ensure preservation of many species typical for grapes. The method of enrichment culture on grape juice medium proved to be more efficient than other methods of analysis with respect to the number of isolated species and the rate of their detection. However, implementation of different techniques to study yeasts' diversity can give somewhat different results; a set of methods should be used for an integrated analysis.

  3. [Environmental geochemical baseline of heavy metals in soils of the Ili river basin and pollution evaluation].

    PubMed

    Zhao, Xin-Ru; Nasier, Telajin; Cheng, Yong-Yi; Zhan, Jiang-Yu; Yang, Jian-Hong

    2014-06-01

    Environmental geochemical baseline models of Cu, Zn, Pb, As, Hg were established by standardized method in the ehernozem, chestnut soil, sierozem and saline soil from the Ili river valley region. The theoretical baseline values were calculated. Baseline factor pollution index evaluation method, environmental background value evaluation method and heavy metal cleanliness evaluation method were used to compare soil pollution degrees. The baseline factor pollution index evaluation showed that As pollution was the most prominent among the four typical types of soils within the river basin, with 7.14%, 9.76%, 7.50% of sampling points in chernozem, chestnut soil and sierozem reached the heavy pollution, respectively. 7.32% of sampling points of chestnut soil reached the permitted heavy metal Pb pollution index in the chestnut soil. The variation extent of As and Pb was the largest, indicating large human disturbance. Environmental background value evaluation showed that As was the main pollution element, followed by Cu, Zn and Pb. Heavy metal cleanliness evaluation showed that Cu, Zn and Pb were better than cleanliness level 2 and Hg was the of cleanliness level 1 in all four types of soils. As showed moderate pollution in sierozem, and it was of cleanliness level 2 or better in chernozem, chestnut soil and saline-alkali soil. Comparing the three evaluation systems, the baseline factor pollution index evaluation more comprehensively reflected the geochemical migration characteristics of elements and the soil formation processes, and the pollution assessment could be specific to the sampling points. The environmental background value evaluation neglected the natural migration of heavy metals and the deposition process in the soil since it was established on the regional background values. The main purpose of the heavy metal cleanliness evaluation was to evaluate the safety degree of soil environment.

  4. Multi-cropping edible truffles and sweet chestnuts: production of high-quality Castanea sativa seedlings inoculated with Tuber aestivum, its ecotype T. uncinatum, T. brumale, and T. macrosporum.

    PubMed

    Álvarez-Lafuente, Amaya; Benito-Matías, Luis F; Peñuelas-Rubira, Juan L; Suz, Laura M

    2018-01-01

    The plantation and management of sweet chestnut (Castanea sativa Mill.) orchards is a common and traditional land use system in many areas of Europe that offers the advantage of simultaneous production of nuts and timber. During the last decades, sweet chestnut has declined dramatically in many regions because of the profound social changes in rural areas coupled with pathogen attacks. Truffles, the hypogeous ascocarps of the ectomycorrhizal genus Tuber, are currently cultivated using host trees inoculated with these fungi for improving production in truffle orchards. The production of good forestry quality chestnut seedlings inoculated with European truffles in nurseries is essential for multi-cropping plantation establishment, but so far, it has not been implemented in agroforestry practices. Moreover, it is necessary to assess the physiological condition of the seedlings due to the high calcium amendment needed for the growth of Tuber spp. mycelium that can become toxic for the host plants. In this study, seedlings of C. sativa were inoculated with Tuber aestivum and its ecotypes T. uncinatum, T. brumale, and T. macrosporum and were grown in a greenhouse using culture conditions favorable for the production of high-quality plants for forestry purposes. At the end of the assay, levels of root colonization and morphological and physiological parameters of the seedlings were measured. The colonization of C. sativa with T. aestivum, its ecotype T. uncinatum, and T. brumale was successful, and the seedlings showed normal growth. Inoculation protocols with T. macrosporum need to be improved. Tuber species formed well-developed ectomycorrhizae on C. sativa in nursery conditions.

  5. Groundwater Protection Program Calendar Year 1998 Groundwater Monitoring Report, U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1999-03-01

    This annual monitoring report contains groundwater and surface water monitoring data obtained during calendar year (CY) 1998 by the Lockheed Martin Energy Systems, Inc. Y-12 Plant Groundwater Protection Program (GWPP) at the U.S. Department of Energy (DOE) Oak Ridge Y-12 Plant, Groundwater and surface water monitoring during CY 1998 was performed in three hydrogeologic regimes at the Y-12 Plant: the Bear Creek Hydrogeologic Regime (Bear Creek Regime), the Chestnut Ridge Hydrogeologic Regime (Chestnut Ridge Regime), and the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime). The Bear Creek and East Fork regimes are located in Bear Creek Valleymore » (BCV), and the Chestnut Ridge Regime which is located south of the Y-12 Plant.« less

  6. 75 FR 76453 - Top of the World Wind Energy, LLC; Kit Carson Windpower, LLC; Chestnut Flats Wind, LLC; Minco...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket Nos. EG10-65-000; EG10-66-000; EG10-67-000; EG10-68-000; EG10- 69-000; EG10-70-000; EG10-71-000] Top of the World Wind Energy, LLC; Kit Carson Windpower, LLC; Chestnut Flats Wind, LLC; Minco Wind, LLC; Arizona Solar One LLC; Criterion...

  7. 1954 Pulpwood production in the south

    Treesearch

    W.S. Stover; J.F. Christopher

    1955-01-01

    In 1954, pulpwood production in the South was 16,269,600 cords, an increase of 0.9 percent over 1953. It was the largest harvest on record. The cut of pine pulpwood was 14,108,000 cords, of hardwood 2,128,800 cords, and of dead chestnut 32,800 cords. While hardwood production was 11.0 percent more than in 1953, pine was 0.3 percent less, and dead chestnut 46.3 percent...

  8. A forest transect of pine mountain, Kentucky: changes since E. Lucy Braun and chestnut blight

    Treesearch

    Tracy S. Hawkins

    2006-01-01

    In 1997, forest composition and structure were determined for Hi Lewis Pine Barrens State Nature Preserve, a 68-ha tract on the south slope of Pine Mountain, Harlan County, Kentucky. Data collected from 28 0.04-ha plots were used to delineate forest types. Percent canopy compositions were compared with those reported by Dr. E. Lucy Braun prior to the peak of chestnut...

  9. Multiple introductions and recombination in Cryphonectria hypovirus 1: perspective for a sustainable biological control of chestnut blight

    PubMed Central

    Feau, Nicolas; Dutech, Cyril; Brusini, Jérémie; Rigling, Daniel; Robin, Cécile

    2014-01-01

    Cryphonectria hypovirus 1 (CHV1) is a mycovirus which decreases the virulence of its fungal host Cryphonectria parasitica, the causal agent of chestnut blight recently introduced in Europe. The understanding of the evolutionary processes which have shaped CHV1 populations in Europe is required to develop a sustainable biocontrol strategy targeting chestnut blight and effective in European chestnut forests. To retrace the evolutionary history of CHV1, we analyzed sequences from two genomic regions on a collection of 55 CHV1 strains from France and northern Spain, two countries where multiple introductions of C. parasitica occurred. Several recombination events and variable selection pressures contributed to CHV1 evolution, agreeing with a non-clock-like diversification rate. These two mechanisms may be at the origin of CHV1 population diversity observed in western Europe. Considering the actual prevalence of CHV1 and its association with host genotypes, multiple introductions of CHV1 may have occurred in Europe, some of them directly from Asia and some of them through North America. Although some viral strains remained with low frequency in their introduction area, multiple infections might have allowed homologous recombination within parental sequences. Some of these recombinant lineages are associated with the spread of CHV1 in European regions. PMID:24944571

  10. AmeriFlux US-Ha2 Harvard Forest Hemlock Site

    DOE Data Explorer

    Munger, William [Harvard University

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site US-Ha2 Harvard Forest Hemlock Site. Site Description - The forest surrounding the Hemlock site has remained pristine with two exceptions. In the early to mid-1700s, European settlers cleared the majority of the forest for agricultural purposes. Selective harvesting of hemlock and chestnut trees occurred up until the early 1900s, when the chestnut blight killed all of the chestnut trees. In the current forest, about 83% of the total basal area of trees is hemlock. The remainder is equally divided between eastern white pine (Pinus strobus L.) and deciduous species, including red maple (Acer rubrum), red oak (Quercus rubra) and black birch (Betula lenta). A very thick organic layer (10-20 cm or more) covers the soil surface, and highly decayed coarse woody debris is abundant.

  11. The influence of super-high-frequency radiation on the enzyme activity and number of microorganisms in soils of southern Russia

    NASA Astrophysics Data System (ADS)

    Denisova, T. V.; Kolesnikov, S. I.

    2009-04-01

    The effects of super-high-frequency radiation (SHF radiation) on the microflora and enzymatic activity of an ordinary chernozem, a chestnut soil, a brown forest soil, and gray sands were studied. The exposure time of the 800-W SHF radiation was 30 s, 1, 10, and 60 min. The activity of the soil enzymes (catalase and invertase) was found to be more resistant to the action of SHF radiation than the number of microorganisms (ammonifying bacteria (including sporogenous ones), bacteria of the genus Azotobacter, and micromycetes). According to the resistance of the enzymes, the soils studied form the following sequence: gray sands > ordinary chernozem ≥ chestnut soil > brown forest soil. Under the action of the SHF radiation, the number of microorganisms in the ordinary chernozem decreased to a lesser extent.

  12. Wheat-water chestnut flour blends: effect of baking on antioxidant properties of cookies.

    PubMed

    Shafi, Musarat; Baba, Waqas N; Masoodi, Farooq Ahmad; Bazaz, Rafiya

    2016-12-01

    Proximate composition, mineral content, functional, pasting and antioxidant properties of water chestnut flour (WCF) were compared with refined wheat flour. WCF showed higher phenolic (4.25 gGAE/1000 g), flavonoid (1.92 g QE/1000 g) and mineral content (K, Mg, Zn, Cu) than wheat flour. WCF showed greater retrogradation tendency but lower peak viscosity than wheat flour. Wheat flour - WCF blends and cookies were evaluated for water activity, physical & textural properties. Water activity of cookies decreased significantly (0.415-0.311) with increase in level of WCF in wheat flour. Total phenolic content, flavonoid content and antioxidant activity (DPPH• scavenging capacity, FRAP) of WCF - wheat flour blends as well as their cookies was also determined. Baking led to a greater increase in DPPH• scavenging capacity of WCF cookies (33.8%) than WF cookies (25%). Baking had a similar effect on FRAP value. Wheat flour cookies showed a decrease of 51%, and 62% while WCF cookies showed a decrease of 36%, and 34% in TPC and TFC values respectively. WCF cookies thus showed better retention of antioxidant activities suggesting greater stability of WC phenolics than wheat phenolics. Sensory analysis showed cookies made from water chestnut (100%) had fair acceptability due to their characteristic flavor. Thus, water chestnut flour serves both as a gluten free as well as antioxidant rich flour for production of cookies.

  13. Cytotoxic triterpenoids isolated from sweet chestnut heartwood (Castanea sativa) and their health benefits implication.

    PubMed

    Pérez, Andy J; Pecio, Łukasz; Kowalczyk, Mariusz; Kontek, Renata; Gajek, Gabriela; Stopinsek, Lidija; Mirt, Ivan; Stochmal, Anna; Oleszek, Wiesław

    2017-11-01

    For centuries wood containers have been used in aging of wines and spirits, due to the pleasant flavors they give to the beverages. Together with oak, sweet chestnut wood (Castanea sativa) have been often used for such purpose. The maturation process involves the transfer of secondary metabolites, mainly phenolics, from the wood to the liquid. At the same time, other metabolites, such as triterpenoids and their glycosides, can also be released. Searching for the extractable triterpenoids from sweet chestnut heartwood (C. sativa), two new ursane-type triterpenoid saponins named chestnoside A (1) and chestnoside B (2), together with two known oleanen-type analogs (3 and 4) were isolated and characterized. The cytotoxicity of isolated compounds was tested against two cancer cell lines (PC3 and MCF-7), and normal lymphocytes. Breast cancer cells (MCF-7) were more affected by tested compounds than prostate cancer cells (PC3). Chestnoside B (2) exhibited the strongest cytotoxicity with an IC 50 of 12.3 μM against MCF-7 cells, lower than those of positive controls, while it was moderately active against normal lymphocytes (IC 50  = 67.2 μM). These results highlight the occurrence of triterpenoid saponins in sweet chestnut heartwood and their potential for the chemoprevention of breast cancer. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Exploration of a rare population of Chinese chestnut in North America: stand dynamics, health and genetic relationships

    PubMed Central

    Miller, Amy C.; Woeste, Keith E.; Anagnostakis, Sandra L.; Jacobs, Douglass F.

    2014-01-01

    With the transport of plants around the globe, exotic species can readily spread disease to their native relatives; however, they can also provide genetic resistance to those relatives through hybrid breeding programmes. American chestnut (Castanea dentata) was an abundant tree species in North America until its decimation by introduced chestnut blight. To restore chestnut in North America, efforts are ongoing to test putative blight-resistant hybrids of Castanea dentata and Chinese chestnut (Castanea mollissima), but little is known about the ecology of C. mollissima. In a forest in northeastern USA in which C. mollissima has become established, we explored questions of stand dynamics, health and genetic relationships of C. mollissima offspring to an adjacent parent orchard. We found that C. mollissima was adapted and randomly distributed among native species in this relatively young forest. The genetics of the C. mollissima population compared with its parents indicated little effect of selection pressure as each of the parent trees contributed at least one offspring. The ease with which this exotic species proliferated calls to question why C. mollissima is rare elsewhere in forests of North America. It is likely that a time window of low animal predation allowed seedlings to establish, and the shallow soil at this site limited the maximum forest canopy height, permitting the characteristically short-statured C. mollissima to avoid suppression. Our results indicate that because C. mollissima exhibited pioneer species characteristics, hybrids between C. mollissima and C. dentata have the potential to be successful pioneer species of future forests in North America, and we challenge the paradigm that exotic tree species are wholly detrimental to native biodiversity. We contend that exotic tree species should be assessed not only by their level of threat to native species, but also by their potential positive impacts on ecosystems via hybrid breeding programmes. PMID:25336337

  15. Exploration of a rare population of Chinese chestnut in North America: stand dynamics, health and genetic relationships.

    PubMed

    Miller, Amy C; Woeste, Keith E; Anagnostakis, Sandra L; Jacobs, Douglass F

    2014-10-20

    With the transport of plants around the globe, exotic species can readily spread disease to their native relatives; however, they can also provide genetic resistance to those relatives through hybrid breeding programmes. American chestnut (Castanea dentata) was an abundant tree species in North America until its decimation by introduced chestnut blight. To restore chestnut in North America, efforts are ongoing to test putative blight-resistant hybrids of Castanea dentata and Chinese chestnut (Castanea mollissima), but little is known about the ecology of C. mollissima. In a forest in northeastern USA in which C. mollissima has become established, we explored questions of stand dynamics, health and genetic relationships of C. mollissima offspring to an adjacent parent orchard. We found that C. mollissima was adapted and randomly distributed among native species in this relatively young forest. The genetics of the C. mollissima population compared with its parents indicated little effect of selection pressure as each of the parent trees contributed at least one offspring. The ease with which this exotic species proliferated calls to question why C. mollissima is rare elsewhere in forests of North America. It is likely that a time window of low animal predation allowed seedlings to establish, and the shallow soil at this site limited the maximum forest canopy height, permitting the characteristically short-statured C. mollissima to avoid suppression. Our results indicate that because C. mollissima exhibited pioneer species characteristics, hybrids between C. mollissima and C. dentata have the potential to be successful pioneer species of future forests in North America, and we challenge the paradigm that exotic tree species are wholly detrimental to native biodiversity. We contend that exotic tree species should be assessed not only by their level of threat to native species, but also by their potential positive impacts on ecosystems via hybrid breeding programmes. Published by Oxford University Press on behalf of the Annals of Botany Company.

  16. A solid-phase extraction procedure coupled to 1H NMR, with chemometric analysis, to seek reliable markers of the botanical origin of honey.

    PubMed

    Beretta, Giangiacomo; Caneva, Enrico; Regazzoni, Luca; Bakhtyari, Nazanin Golbamaki; Maffei Facino, Roberto

    2008-07-14

    The aim of this work was to establish an analytical method for identifying the botanical origin of honey, as an alternative to conventional melissopalynological, organoleptic and instrumental methods (gas-chromatography coupled to mass spectrometry (GC-MS), high-performance liquid chromatography HPLC). The procedure is based on the (1)H nuclear magnetic resonance (NMR) profile coupled, when necessary, with electrospray ionisation-mass spectrometry (ESI-MS) and two-dimensional NMR analyses of solid-phase extraction (SPE)-purified honey samples, followed by chemometric analyses. Extracts of 44 commercial Italian honeys from 20 different botanical sources were analyzed. Honeydew, chestnut and linden honeys showed constant, specific, well-resolved resonances, suitable for use as markers of origin. Honeydew honey contained the typical resonances of an aliphatic component, very likely deriving from the plant phloem sap or excreted into it by sap-sucking aphids. Chestnut honey contained the typical signals of kynurenic acid and some structurally related metabolite. In linden honey the (1)H NMR profile gave strong signals attributable to the mono-terpene derivative cyclohexa-1,3-diene-1-carboxylic acid (CDCA) and to its 1-O-beta-gentiobiosyl ester (CDCA-GBE). These markers were not detectable in the other honeys, except for the less common nectar honey from rosa mosqueta. We compared and analyzed the data by multivariate techniques. Principal component analysis found different clusters of honeys based on the presence of these specific markers. The results, although obviously only preliminary, suggest that the (1)H NMR profile (with HPLC-MS analysis when necessary) can be used as a reference framework for identifying the botanical origin of honey.

  17. Honey shows potent inhibitory activity against the bovine testes hyaluronidase.

    PubMed

    Kolayli, Sevgi; Sahin, Huseyin; Can, Zehra; Yildiz, Oktay; Sahin, Kübra

    2016-08-01

    The purpose of this study was to investigate the anti-hyaluronidase activities of honeys from different botanical origins honeys in order to determine their anti-inflammatory properties. The total phenolic contents, total flavonoids and total tannin levels of six types of honey, chestnut, oak, heather, pine, buckwheat and mixed blossom, were determined. Concentration-related inhibition values were tested turbidimetrically on bovine testis hyaluronidase (BTHase) as IC50 (mg/mL). All honeys exhibited various concentration-dependent degrees of inhibition against BTHase. Inhibition values varied significantly depending on honeys' levels of phenolic contents, flavonoid and tannin. The honeys with the highest anti-hyaluronidase activity were oak, chestnut and heather. In conclusion, polyphenol-rich honeys have high anti-hyaluronidase activity, and these honeys have high protective and complementary potential against hyaluronidase-induced anti-inflammatory failures.

  18. The characterization and properties of castaprenol-11, -12 and -13 from the leaves of Aesculus hippocastanum (horse chestnut)

    PubMed Central

    Wellburn, A. R.; Stevenson, J.; Hemming, F. W.; Morton, R. A.

    1967-01-01

    The isolation and purification of a mixture of cis–trans-polyprenols from the leaves of Aesculus hippocastanum (horse chestnut) are described. Results of studies involving mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, micro-hydrogenation and ozonolytic degradation show the mixture to be made up of undecaprenol, dodecaprenol and tridecaprenol with dodecaprenol predominating. Each of the prenols contains three trans internal isoprene residues and a cis `OH-terminal' isoprene residue. They differ from each other only in the number of cis internal isoprene residues. The trivial names castaprenol-11, castaprenol-12 and castaprenol-13 are proposed to describe these compounds. Gas–liquid-chromatographic and reversed-phase partition thin-layer chromatographic evidence suggest the presence in the mixture of small quantities of castaprenol-10 also. PMID:6067665

  19. Lichenized fungi of a chestnut grove in Livari (Rumija, Montenegro).

    PubMed

    Mayrhofer, Helmut; Drescher, Anton; Stešević, Danijela; Bilovitz, Peter O

    2013-10-08

    Sixty taxa (59 species and 1 variety) of lichenized fungi are reported from a chestnut grove in Livari. The majority of them (55 species and 1 variety) occurred on Castanea sativa . The recently described Xylographa soralifera is new to the Balkan Peninsula. The lichenicolous fungus Monodictys epilepraria growing on Lepraria rigidula is new to Montenegro. The lichen mycota is compared with similar localities in Italy and Switzerland. The species composition in Livari is most similar to the Montieri site in Tuscany.

  20. Lichenized fungi of a chestnut grove in Livari (Rumija, Montenegro)

    PubMed Central

    Mayrhofer, Helmut; Drescher, Anton; Stešević, Danijela; Bilovitz, Peter O.

    2016-01-01

    Sixty taxa (59 species and 1 variety) of lichenized fungi are reported from a chestnut grove in Livari. The majority of them (55 species and 1 variety) occurred on Castanea sativa. The recently described Xylographa soralifera is new to the Balkan Peninsula. The lichenicolous fungus Monodictys epilepraria growing on Lepraria rigidula is new to Montenegro. The lichen mycota is compared with similar localities in Italy and Switzerland. The species composition in Livari is most similar to the Montieri site in Tuscany. PMID:26869743

  1. Abundance and Frequency of the Asiatic Oak Weevil (Coleoptera: Curculionidae) and Defoliation on American, Chinese, and Hybrid Chestnut (Castanea)

    PubMed Central

    Case, Ashley E.; Mayfield, Albert E.; Clark, Stacy L.; Schlarbaum, Scott E.; Reynolds, Barbara C.

    2016-01-01

    The Asiatic oak weevil, Cyrtepistomus castaneus Roelofs (Coleoptera: Curculionidae), is a nonnative defoliator of trees in the Fagaceae family in the United States but has not been studied on Castanea species in the southern Appalachian Mountains. Planted trees of Castanea dentata (Marsh.) Borkh. (Fagales: Fagaceae), Castanea mollissima Blume (Fagales: Fagaceae), and four hybrid breeding generations were evaluated in 2012 for insect defoliation and C. castaneus abundance and frequency. Defoliation was visually assessed throughout the growing season at two sites in the southern Appalachian Mountains (western North Carolina and eastern Tennessee). C. castaneus abundance and frequency were monitored on trees using beat sheets and emergence was recorded from ground traps. Asiatic oak weevils were more abundant and more frequently collected on American chestnut (Ca. dentata) and its most closely related BC3F3 hybrid generation than on the Asian species Ca. mollissima. In most months, C. castaneus colonization of hybrid generations was not significantly different than colonization of parental species. Frequency data for C. castaneus suggested that adults were distributed relatively evenly throughout the study sites rather than in dense clusters. Emergence of C. castaneus was significantly higher under a canopy dominated by Quercus species than under non-Quercus species or open sky. C. castaneus emergence began in May and peaked in late June and early July. These results may be useful for resource managers trying to restore blight-resistant chestnut to the Southern Appalachians while minimizing herbivory by insect pests. PMID:27001964

  2. 42 CFR 85.3 - Procedures for requesting health hazard evaluations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... OCCUPATIONAL SAFETY AND HEALTH RESEARCH AND RELATED ACTIVITIES REQUESTS FOR HEALTH HAZARD EVALUATIONS § 85.3..., and Field Studies, NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226. (2) Requests from mining industry. Environmental Investigations Branch, Division of Respiratory Disease Studies, NIOSH, 944 Chestnut...

  3. 42 CFR 85.3 - Procedures for requesting health hazard evaluations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... OCCUPATIONAL SAFETY AND HEALTH RESEARCH AND RELATED ACTIVITIES REQUESTS FOR HEALTH HAZARD EVALUATIONS § 85.3..., and Field Studies, NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226. (2) Requests from mining industry. Environmental Investigations Branch, Division of Respiratory Disease Studies, NIOSH, 944 Chestnut...

  4. 42 CFR 85.3 - Procedures for requesting health hazard evaluations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... OCCUPATIONAL SAFETY AND HEALTH RESEARCH AND RELATED ACTIVITIES REQUESTS FOR HEALTH HAZARD EVALUATIONS § 85.3..., and Field Studies, NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226. (2) Requests from mining industry. Environmental Investigations Branch, Division of Respiratory Disease Studies, NIOSH, 944 Chestnut...

  5. 42 CFR 85.3 - Procedures for requesting health hazard evaluations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... OCCUPATIONAL SAFETY AND HEALTH RESEARCH AND RELATED ACTIVITIES REQUESTS FOR HEALTH HAZARD EVALUATIONS § 85.3..., and Field Studies, NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226. (2) Requests from mining industry. Environmental Investigations Branch, Division of Respiratory Disease Studies, NIOSH, 944 Chestnut...

  6. 42 CFR 85.3 - Procedures for requesting health hazard evaluations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... OCCUPATIONAL SAFETY AND HEALTH RESEARCH AND RELATED ACTIVITIES REQUESTS FOR HEALTH HAZARD EVALUATIONS § 85.3..., and Field Studies, NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226. (2) Requests from mining industry. Environmental Investigations Branch, Division of Respiratory Disease Studies, NIOSH, 944 Chestnut...

  7. Paleoecological crisis in the steppes of the Lower Volga region in the Middle of the Bronze Age (III-II centuries BC)

    NASA Astrophysics Data System (ADS)

    Demkina, T. S.; Borisov, A. V.; Demkin, V. A.; Khomutova, T. E.; Kuznetsova, T. V.; El'tsov, M. V.; Udal'tsov, S. N.

    2017-07-01

    Diagnostic features of a catastrophic aridization of climate, desertification, and paleoecological crisis in steppes of the Lower Volga region have been identified on the basis of data on the morphological, chemical, and microbiological properties of paleosols under archeological monuments (burial mounds) of the Middle Bronze Age. These processes resulted in a certain convergence of the soil cover with transformation of zonal chestnut (Kastanozems) paleosols and paleosolonetzes (Solonetz Humic) into specific chestnut-like eroded saline calcareous paleosols analogous to the modern brown desert-steppe soils (Calcisols Haplic) that predominated in this region 4300-3800 years ago.1 In the second millennium BC, humidization of the climate led to the divergence of the soil cover with secondary formation of the complexes of chestnut soils and solonetzes. This paleoecological crisis had a significant effect on the economy of the tribes in the Late Catacomb and Post-Catacomb time stipulating their higher mobility and transition to the nomadic cattle breeding.

  8. Calendar year 1993 groundwater quality report for the Chestnut Ridge Hydrogeologic Regime, Y-12 Plant, Oak Ridge, Tennessee. 1993 groundwater quality data and calculated rate of contaminant migration, Part 1

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1994-02-01

    This annual groundwater report contains groundwater quality data obtained during the 1993 calendar year (CY) at several hazardous and non-hazardous waste-management facilities associated with the US Department of Energy (DOE) Y-12 Plant located on the DOE Oak Ridge Reservation (ORR) southeast of Oak Ridge, Tennessee. These sites are located south of the Y-12 Plant in the Chestnut Ridge Hydrogeologic Regime (Chestnut Ridge Regime), which is one of three regimes defined for the purposes of groundwater quality monitoring at the Y-12 Plant. The Environmental Management Department of the Y-12 Plant Health, Safety, Environment, and Accountability Organization manages the groundwater monitoring activitiesmore » in each regime as part of the Y-12 Plant Groundwater Protection Program (GWPP). The annual groundwater report for the Chestnut Ridge Regime is completed in two-parts; Part 1 (this report) containing the groundwater quality data and Part 2 containing a detailed evaluation of the data. The primary purpose of this report is to serve as a reference for the groundwater quality data obtained each year under the lead of the Y-12 Plant GWPP. However, because it contains information needed to comply with Resource Conservation and Recovery Act (RCRA) interim status assessment monitoring and reporting requirements, this report is submitted to the Tennessee Department of Health and Environment (TDEC) by the RCRA reporting deadline.« less

  9. Hierarchical chestnut-like MnCo2O4 nanoneedles grown on nickel foam as binder-free electrode for high energy density asymmetric supercapacitors

    NASA Astrophysics Data System (ADS)

    Hui, Kwun Nam; Hui, Kwan San; Tang, Zikang; Jadhav, V. V.; Xia, Qi Xun

    2016-10-01

    Hierarchical chestnut-like manganese cobalt oxide (MnCo2O4) nanoneedles (NNs) are successfully grown on nickel foam using a facile and cost-effective hydrothermal method. High resolution TEM image further verifies that the chestnut-like MnCo2O4 structure is assembled by numerous 1D MnCo2O4 nanoneedles, which are formed by numerous interconnected MnCo2O4 nanoparticles with grain diameter of ∼10 nm. The MnCo2O4 electrode exhibits high specific capacitance of 1535 F g-1 at 1 A g-1 and good rate capability (950 F g-1 at 10 A g-1) in a 6 M KOH electrolyte. An asymmetric supercapacitor is fabricated using MnCo2O4 NNs on Ni foam (MnCo2O4 NNs/NF) as the positive electrode and graphene/NF as the negative electrode. The device shows an operation voltage of 1.5 V and delivers a high energy density of ∼60.4 Wh kg-1 at a power density of ∼375 W kg-1. Moreover, the device exhibits an excellent cycling stability of 94.3% capacitance retention after 12000 cycles at 30 A g-1. This work demonstrates that hierarchical chestnut-like MnCo2O4 NNs could be a promising electrode for the high performance energy storage devices.

  10. Determination of neonicotinoid pesticides residues in agricultural samples by solid-phase extraction combined with liquid chromatography-tandem mass spectrometry.

    PubMed

    Xie, Wen; Han, Chao; Qian, Yan; Ding, Huiying; Chen, Xiaomei; Xi, Junyang

    2011-07-15

    This work reports a new sensitive multi-residue liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detection, confirmation and quantification of six neonicotinoid pesticides (dinotefuran, thiamethoxam, clothiandin, imidacloprid, acetamiprid and thiacloprid) in agricultural samples (chestnut, shallot, ginger and tea). Activated carbon and HLB solid-phase extraction cartridges were used for cleaning up the extracts. Analysis is performed by LC-MS/MS operated in the multiple reaction monitoring (MRM) mode, acquiring two specific precursor-product ion transitions per target compound. Quantification was carried by the internal standard method with D(4)-labeled imidacloprid. The method showed excellent linearity (R(2)≥0.9991) and precision (relative standard deviation, RSD≤8.6%) for all compounds. Limits of quantification (LOQs) were 0.01 mg kg(-1) for chestnut, shallot, ginger sample and 0.02 mg kg(-1) for tea sample. The average recoveries, measured at three concentrations levels (0.01 mg kg(-1), 0.02 mg kg(-1) and 0.1 mg kg(-1) for chestnut, shallot, ginger sample, 0.02 mg kg(-1), 0.04 mg kg(-1) and 0.2 mg kg(-1) for tea sample), were in the range 82.1-108.5%. The method was satisfactorily validated for the analysis of 150 agricultural samples (chestnut, shallot, ginger and tea). Imidacloprid and acetamiprid were detected at concentration levels ranging from 0.05 to 3.6 mg kg(-1). Copyright © 2011 Elsevier B.V. All rights reserved.

  11. The chestnut blight fungus for studies on virus/host and virus/virus interactions: from a natural to a model host.

    PubMed

    Eusebio-Cope, Ana; Sun, Liying; Tanaka, Toru; Chiba, Sotaro; Kasahara, Shin; Suzuki, Nobuhiro

    2015-03-01

    The chestnut blight fungus, Cryphonectria parasitica, is an important plant pathogenic ascomycete. The fungus hosts a wide range of viruses and now has been established as a model filamentous fungus for studying virus/host and virus/virus interactions. This is based on the development of methods for artificial virus introduction and elimination, host genome manipulability, available host genome sequence with annotations, host mutant strains, and molecular tools. Molecular tools include sub-cellular distribution markers, gene expression reporters, and vectors with regulatable promoters that have been long available for unicellular organisms, cultured cells, individuals of animals and plants, and certain filamentous fungi. A comparison with other filamentous fungi such as Neurospora crassa has been made to establish clear advantages and disadvantages of C. parasitica as a virus host. In addition, a few recent studies on RNA silencing vs. viruses in this fungus are introduced. Copyright © 2014 Elsevier Inc. All rights reserved.

  12. The Fractal Nature of Wood Revealed by Drying

    DTIC Science & Technology

    2000-01-01

    structure of pore space. Take the natural 298 Table 1. The fractal dimensions of Ginkgo and Chinese chestnut obtained at varable temperatures species 200C 400...C 600C 800C 1000C Ginkgo 2.106 2.547 2.851 2.863 2.876 Chinese chestnut 2.008 2.566 2.814 2.896 2.972 logarithm of both sides of Eq. (2) and we have...The materials for this investigation came from two species, one was a 37-year- old plantation-grown Ginkgo ( Ginkgo biloba) and the other was a 48-year

  13. Remedial investigation report on Chestnut Ridge Operable Unit 2 (filled coal ash pond/Upper McCoy Branch) at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. Volume 2: Appendixes

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1994-08-01

    This report comprises appendices A--J which support the Y-12 Plant`s remedial action report involving Chestnut Ridge Operable Unit 2 (filled coal ash pond/Upper McCoy Branch). The appendices cover the following: Sampling fish from McCoy Branch; well and piezometer logs; ecological effects of contaminants in McCoy Branch 1989-1990; heavy metal bioaccumulation data; microbes in polluted sediments; and baseline human health risk assessment data.

  14. Leaf physiology and biomass allocation of backcross hybrid American chestnut (Castanea dentata) seedlings in response to light and water availability.

    PubMed

    Brown, Caleb E; Mickelbart, Michael V; Jacobs, Douglass F

    2014-12-01

    Partial canopy cover promotes regeneration of many temperate forest trees, but the consequences of shading on seedling drought resistance are unclear. Reintroduction of blight-resistant American chestnut (Castanea dentata (Marsh.) Borkh.) into eastern North American forests will often occur on water-limited sites and under partial canopy cover. We measured leaf pre-dawn water potential (Ψpd), leaf gas exchange, and growth and biomass allocation of backcross hybrid American chestnut seedlings from three orchard sources grown under different light intensities (76, 26 and 8% full photosynthetically active radiation (PAR)) and subjected to well-watered or mid-season water-stressed conditions. Seedlings in the water-stress treatment were returned to well-watered conditions after wilting to examine recovery. Seedlings growing under medium- and high-light conditions wilted at lower leaf Ψpd than low-light seedlings. Recovery of net photosynthesis (Anet) and stomatal conductance (gs) was greater in low and medium light than in high light. Seed source did not affect the response to water stress or light level in most cases. Between 26 and 8% full PAR, light became limiting to the extent that the effects of water stress had no impact on some growth and morphological traits. We conclude that positive and negative aspects of shading on seedling drought tolerance and recovery are not mutually exclusive. Partial shade may help American chestnut tolerate drought during early establishment through effects on physiological conditioning. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  15. Compositional analysis of Chinese water chestnut (Eleocharis dulcis) cell-wall material from parenchyma, epidermis, and subepidermal tissues.

    PubMed

    Grassby, Terri; Jay, Andrew J; Merali, Zara; Parker, Mary L; Parr, Adrian J; Faulds, Craig B; Waldron, Keith W

    2013-10-09

    Chinese water chestnut (Eleocharis dulcis (Burman f.) Trin ex Henschel) is a corm consumed globally in Oriental-style cuisine. The corm consists of three main tissues, the epidermis, subepidermis, and parenchyma; the cell walls of which were analyzed for sugar, phenolic, and lignin content. Sugar content, measured by gas chromatography, was higher in the parenchyma cell walls (931 μg/mg) than in the subepidermis (775 μg/mg) or epidermis (685 μg/mg). The alkali-extractable phenolic content, measured by high-performance liquid chromatography, was greater in the epidermal (32.4 μg/mg) and subepidermal cell walls (21.7 μg/mg) than in the cell walls of the parenchyma (12.3 μg/mg). The proportion of diferulic acids was higher in the parenchyma. The Klason lignin content of epidermal and subepidermal cell walls was ~15%. Methylation analysis of Chinese water chestnut cell-wall polysaccharides identified xyloglucan as the predominant hemicellulose in the parenchyma for the first time, and also a significant pectin component, similar to other nongraminaceous monocots.

  16. Castanea sativa Mill. Flowers amongst the Most Powerful Antioxidant Matrices: A Phytochemical Approach in Decoctions and Infusions

    PubMed Central

    Carocho, Márcio; Barros, Lillian; Bento, Albino; Morales, Patricia; Ferreira, Isabel C. F. R.

    2014-01-01

    Infusions and decoction of chestnut tree flowers have been used for different medical purposes, but their phytochemical profile and antioxidant activity are still mostly unknown. Herein, decoctions and infusions of flowers from the two most appreciated chestnut cultivars (longal and judia) in Trás-os-Montes, Portugal, were prepared and characterized with regard to their content in free sugars, organic acids, and phenolic compounds, such as flavonoids and hydrolyzable tannins, and their antioxidant activity. Overall, the decoction of the cultivar judia was the sample with both the highest quantity of flavonoids and antioxidant activity. The phenolic compound with the highest abundance in all samples was trigalloyl-HHDP-glucoside, followed by pentagalloyl glucoside. The sample with the highest quantity of total phenolic compounds was judia infusion, closely followed by longal decoction, which also gave the highest quantities of ellagitannins. Regarding sugars and organic acids, the profiles were more similar. These results corroborate ancestral claims of the health benefits of infusions and decoctions of chestnut flowers. PMID:24822186

  17. A cytoplasmically transmissible hypovirulence phenotype associated with mitochondrial DNA mutations in the chestnut blight fungus Cryphonectria parasitica.

    PubMed Central

    Monteiro-Vitorello, C B; Bell, J A; Fulbright, D W; Bertrand, H

    1995-01-01

    Mutations causing mitochondrial defects were induced in a virulent strain of the chestnut blight fungus Cryphonectria parasitica (Murr.) Barr. Virulence on apples and chestnut trees was reduced in four of six extensively characterized mutants. Relative to the virulent progenitor, the attenuated mutants had reduced growth rates, abnormal colony morphologies, and few asexual spores, and they resembled virus-infected strains. The respiratory defects and attenuated virulence phenotypes (hypovirulence) were transmitted from two mutants to a virulent strain by hyphal contact. The infectious transmission of hypovirulence occurred independently of the transfer of nuclei, did not involve a virus, and dynamically reflects fungal diseases caused by mitochondrial mutations. In these mutants, mitochondrial mutations are further implicated in generation of the attenuated state by (i) uniparental (maternal) inheritance of the trait, (ii) presence of high levels of cyanide-insensitive mitochondrial alternative oxidase activity, (iii) cytochrome deficiencies, and (iv) structural abnormalities in the mtDNA. Hence, cytoplasmically transmissible hypovirulence phenotypes found in virus-free strains of C. parasitica from recovering trees may be caused by mutant forms of mtDNA. Images Fig. 2 Fig. 4 PMID:11607549

  18. Diameter increase in second-growth Appalachian hardwood stands - a comparison of species

    Treesearch

    George R., Jr. Trimble

    1967-01-01

    A study of growth at d.b.h. among eight hardwood species after partial cutting in second-growth stands. Red oak grew fastest, followed in order by yellow-poplar, sugar maple, basswood, black cherry, white ash, beech, and chestnut oak.

  19. Powdered hide model for vegetable tanning II. hydrolyzable tannin

    USDA-ARS?s Scientific Manuscript database

    Vegetable tannages employ both condensed and hydrolyzable tannins. As part of our exploration of tanning mechanisms, we reported last year on interactions of the condensed tannin, quebracho, with powdered hide. In this study, the interactions of chestnut extract, a hydrolyzable tannin, with powdere...

  20. Cryopreservation of embryogenic callus of Aesculus hippocastanum L. by vitrification or one-step freezing.

    PubMed

    Lambardi, Maurizio; De Carlo, Anna; Capuana, Maurizio

    2005-01-01

    An effective procedure for the cryopreservation of horse chestnut (Aesculus hippocastanum L.) embryogenic callus by vitrification/one-step freezing is described here. In particular, the study focused on the possibility of recovering the full proliferation potential of the embryogenic lines after storage in liquid nitrogen. The developmental stage of the embryogenic lines was shown to play an important role. Ninety-min incubation in PVS2 and preservation at -196 degrees C of callus samples, containing a prevalence of embryogenic masses at an advanced stage of somatic embryo maturation (i.e., the torpedo stage), gave optimum regrowth of healthy and proliferating embryogenic callus. Moreover, raising the thawing temperature to 45 degrees C yielded the maximum survival (94%) of torpedo-stage embryogenic samples, recovery of proliferation and, in more than 70% of cases, maturation to the cotyledonary stage. This study opens the way to the possibility of safe, long-term storage in liquid nitrogen of valuable embryogenic lines of horse chestnut, avoiding repeated subculturing.

  1. An investigation of Turkish honeys: their physico-chemical properties, antioxidant capacities and phenolic profiles.

    PubMed

    Can, Zehra; Yildiz, Oktay; Sahin, Huseyin; Akyuz Turumtay, Emine; Silici, Sibel; Kolayli, Sevgi

    2015-08-01

    This study investigated some physico-chemical and biochemical characteristics of different honey types belonging to Turkish flora. Sixty-two honey samples were examined on the basis of pollen analyses, including 11 unifloral honeys (chestnut, heather, chaste tree, rhododendron, common eryngo, lavender, Jerusalem tea, astragalus, clover and acacia), two different honeydew honeys (lime and oak), and 7 different multifloral honeys. Electrical conductivity, moisture, Hunter color values, HMF, proline, diastase number, and sugar analyses of the honey samples were assessed for chemical characterization. Some phenolic components were analyzed by reverse phase high performance liquid chromatography (RP-HPLC) to determine honeys' phenolic profiles. Total phenolic compounds, total flavonoids, ferric reducing antioxidant capacity (FRAP) and 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity were measured as antioxidant determinants. The study results confirm that physico-chemical and biological characteristics of honeys are closely related to their floral sources, and that dark-colored honeys such as oak, chestnut and heather, have a high therapeutic potential. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Dissolution and fractionation of nut shells in ionic liquids.

    PubMed

    Carneiro, Aristides P; Rodríguez, Oscar; Macedo, Eugénia A

    2017-03-01

    The aim of this work was to study the dissolution of raw peanut and chestnut shells in ionic liquids. Dissolution of raw biomass up to 7wt% was achieved under optimized operatory conditions. Quantification of polysaccharides dissolved through quantitative 13 Cq NMR revealed extractions of the cellulosic material to ionic liquids as high as 87%. Regeneration experiments using an antisolvent mixture allowed to recover the cellulosic material and the ionic liquid. The overall mass balance presented very low loss rates (<8%), recoveries of 75% and 95% of cellulosic material from peanut and chestnut shells, respectively, and the recovery of more than 95% of the ionic liquid in both cases. These results show the high potential of using nut shells and ionic liquids for biorefining purposes. Moreover, high recovery of ionic liquids favors the process from an economical point of view. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Determining the Leaf Emissivity of Three Crops by Infrared Thermometry

    PubMed Central

    Chen, Chiachung

    2015-01-01

    Plant temperature can provide important physiological information for crop management. Non-contact measurement with an infrared thermometer is useful for detecting leaf temperatures. In this study, a novel technique was developed to measure leaf emissivity using an infrared thermometer with an infrared sensor and a thermocouple wire. The measured values were transformed into true temperatures by calibration equations to improve the measurement accuracy. The relationship between two kinds of measurement temperatures and setting emissivities was derived as a model for calculating of true emissivity. The emissivities of leaves of three crops were calculated by the mathematical equation developed in this study. The mean emissivities were 0.9809, 0.9783, 0.981 and 0.9848 for Phalaenopsis mature and new leaves and Paphiopedilum and Malabar chestnut leaves, respectively. Emissivity differed significantly between leaves of Malabar chestnut and the two orchids. The range of emissivities determined in this study was similar to that in the literature. The precision of the measurement is acceptable. The method developed in this study is a real-time, in situ technique and could be used for agricultural and forestry plants. PMID:25988870

  4. Somatic Embryogenesis in Horse Chestnut (Aesculus hippocastanum L.).

    PubMed

    Capuana, Maurizio

    2016-01-01

    Embryogenic cultures of horse chestnut (Aesculus hippocastanum L.) can be obtained from different organs and tissues. We describe here the induction from stamen filaments and the procedures applied for the successive phases of somatic embryo development and maturation. Embryogenic tissues are obtained on Murashige and Skoog medium containing 9.0 μM 2,4-dichlorophenoxyacetic acid. Somatic embryos develop after transfer to hormone-free medium enriched with glutamine. Maturation and germination of isolated embryos are achieved by transfer to medium containing polyethylene glycol 4000 and activated charcoal, successive desiccation treatment, and cold storage at 4 °C for 8 weeks.

  5. Spectroscopy analysis of phenolic and sugar patterns in a food grade chestnut tannin.

    PubMed

    Ricci, A; Lagel, M-C; Parpinello, G P; Pizzi, A; Kilmartin, P A; Versari, A

    2016-07-15

    Tannin of chestnut (Castanea sativa Mill.) wood, commonly used in winemaking was characterised with a spectroscopy qualitative approach that revealed its phenolic composition: several vibrational diagnostic bands assigned using the Attenuated Total Reflectance-Infrared Spectroscopy, and fragmentation patterns obtained using the Laser-Desorption-Ionization Time-of-Flight technique evidenced polygalloylglucose, e.g. castalagin/vescalagin-like structures as the most representative molecules, together with sugar moieties. The implication of these findings on winemaking application and the potential influence of the chemical structure on the sensory properties of wine are discussed. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. The Suicide of Marigold Perry.

    ERIC Educational Resources Information Center

    Berman, Alan L., Ed.

    1992-01-01

    Presents case study of 50-year-old widow with history of emotional disturbance who committed suicide by a drug overdose 10 days after discharge from inpatient treatment for psychotic illness. Following case presentation are commentaries from John Maltsberger, MD (McLean Hospital, Belmont, Massachusetts) and Wayne Fenton, MD (Chestnut Lodge…

  7. Fabrication Studies of Ternary Rare Earth Sulfides for Infrared Applications.

    DTIC Science & Technology

    1982-05-01

    crystal growth by vapor transport. The objective was to achieve small , high purity, theoretically dense crystals for optical property characterization...Weapons Center White Oak Silver Spring MD 20910 Dr. S. Musikant General Electric Co - RESD 3198 Chestnut Street Philadelphia PA 19101 Dr. W. White

  8. The effect of chestnut coppice forests abandon on slope stability: a case study

    NASA Astrophysics Data System (ADS)

    Vergani, Chiara; Bassanelli, Chiara; Rossi, Lorenzo; Chiaradia, Enrico Antonio; Battista Bischetti, Gian

    2013-04-01

    Sweet chestnut has been fundamental for Italian mountainous economies for many centuries. This kind of forest was traditionally managed by coppicing in shortly rotation (15-20 years) to rapidly produce wood biomass until half of XX century. In the last decades these forests were in large part abandoned due to change in economy which made coppiced forest management unprofitable, especially in steeper slopes and where forest viability is scarce. As a consequence most of them are over aged and very dense, leading to an observed increasing in localized slope instability, primary because of the uprooting of stools (Vogt et al., 2006). In this work the effect of the abandon of chestnut coppice on slope stability was analyzed, focusing on shallow landslides triggering. The mechanical contribution to soil shear strength of differently managed chestnut stand was estimated and compared in terms of additional root cohesion. The study area is located in the Valcuvia Valley (Lombardy Prealps - Northern Italy) at an elevation about 600 m a.s.l., where two different stands, one managed and the other abandoned (over 40 year aged), were chosen. The two sampling stands are on cohesionless slopes (quaternary moraine deposits) and are homogeneous with regard to the substrate, exposure and elevation. Slope steepness influences heavily forestry practices and steeper stands are more frequently abandoned than stands on gentler terrain: in fact in the abandoned coppice the slope was higher (35 degrees against 13 in the managed stand) and no stands completely homogeneous can be found. In each site the main characteristics of the stand were surveyed and a trench in each stand was excavated to analyze root diameter and number distribution with depth; root specimens were also collected for the tensile force determination through laboratory tensile tests. Root distribution and force were then used to estimate root cohesion values through a Fiber Boundle Model (Pollen and Simon, 2005). Results, as expected, show that management didn't affect root mechanical properties, whereas root distribution within the soil profile did. In terms of additional root cohesion, values are higher in the managed stand, and lower in the abandoned one, at least in the first 50 cm of soil. In the abandoned stand, in fact, roots reach deeper layers of soil (100 cm) than the managed one (50 cm), mainly because of an unexpected greater soil depth. To assess the implication of such results in terms of slope stability, a simple infinite slope model was applied to the two conditions. The results showed that the abandoned stand is prone to instability also with a low level of saturation. On the contrary, by applying the additional root cohesion profile obtained in the managed stand to the steeper slopes, stability should be guaranteed, except in the case of total saturation. In conclusion, although more investigations are required especially to extend the number of stands, coppicing practice seem to be fundamental to prevent shallow landsliding in sweet chestnut forests over cohesionless slopes.

  9. An in vitro evaluation of the effects of a Yucca schidigera extract and chestnut tannins on composition and metabolic profiles of canine and feline faecal microbiota.

    PubMed

    Pinna, Carlo; Vecchiato, Carla Giuditta; Cardenia, Vladimiro; Rodriguez-Estrada, Maria Teresa; Stefanelli, Claudio; Grandi, Monica; Gatta, Pier Paolo; Biagi, Giacomo

    2017-10-01

    The in vitro effect of a Yucca schidigera extract (YSE) and tannins from chestnut wood on composition and metabolic activity of canine and feline faecal microbiota was evaluated. Four treatments were carried out: control diet, chestnut tannins (CT), YSE and CT + YSE. The YSE was added to canine and feline faecal cultures at 0.1 g/l, while CT were added at 0.3 g/l for a 24-h incubation. A total of 130 volatile compounds were detected by means of headspace-solid phase microextraction gas-chromatography/mass spectrometry analyses. Several changes in the metabolite profiles of fermentation fluids were found, including a decrease of alcohols (-19%) and esters (-42%) in feline and canine inoculum, respectively, which was due to the antibacterial properties of tannins. In canine inoculum, after 6 h, YSE + CT caused lower cadaverine concentrations (-37%), while ammonia (-4%) and quinolone (-27%) were reduced by addition of CT. After 24 h, the presence of CT resulted in a decrease of sulphur compounds, such as dimethyl sulphide (-69%) and dimethyl disulphide (-20%). In feline faecal cultures, after 6 h, CT lowered the amount of indole (-48%), whereas YSE tended to decrease trimethylamine levels (-16%). Both in canine and feline inoculum, addition of CT and, to a minor extent, YSE affected volatile fatty acids patterns. In canine faecal cultures, CT exerted a marginal inhibitory effect on Escherichia coli population (-0.45 log 10 numbers of DNA copies/ml), while enterococci were increased (+2.06 log 10 numbers of DNA copies/ml) by YSE. The results from the present study show that YSE and tannins from chestnut wood exert different effects on the composition and metabolism of canine and feline faecal microbiota. In particular, the supplementation of YSE and tannins to diets for dogs and cats may be beneficial due to the reduction of the presence of some potentially toxic volatile metabolites in the animals' intestine.

  10. Protective Effect of Highly Polymeric A-Type Proanthocyanidins from Seed Shells of Japanese Horse Chestnut (Aesculus turbinata BLUME) against Light-Induced Oxidative Damage in Rat Retina

    PubMed Central

    Ishihara, Tomoe; Kaidzu, Sachiko; Kimura, Hideto; Koyama, Yasurou; Matsuoka, Yotaro

    2018-01-01

    Retinal tissue is exposed to oxidative stress caused by visible light. Light-damaged rat used in age-related macular degeneration (AMD) studies clarified that antioxidants decrease retinal light damage. Albino rats were exposed to 5000 Lux light for 12 h with oral administration of the polyphenolic compounds fraction (PF) from the seed shells of Japanese horse chestnut (30 mg/kg, 100 mg/kg, and 300 mg/kg body weight: BW). To evaluate the protective effects against light damage, electroretinograms (ERGs), the outer nuclear layer (ONL) thickness, the antioxidant activity of plasma, oxidized retinal lipids, and the detection of apoptosis were examined. To reveal their active compounds, PF were separated into an A-type proanthocyanidin (PAF) and a flavonol O-glycosides fraction. The protective effects of these fractions against light damage were compared by measuring the thickness of the ERGs and ONL. Compared with the negative control, the PF group (100 mg/kg and 300 mg/kg BW) significantly suppressed the decrease of the ERG amplitudes and ONL thickness. PF (300 mg/kg BW) induced the elevation of in vivo antioxidant activity, and the suppression of retinal lipid oxidation. PF administration also suppressed apoptotic cell death. The protective effects against light damage were attributable to the antioxidant activity of PAF. The light-induced damage of retinas was protected by oral administration of PF and PAF. Taken together, these compounds are potentially useful for the prevention of the disease caused by light exposure. Highlights: The protective effects of retinal damage by light exposure were evaluated using polyphenolic compounds from the seed shells of Japanese horse chestnut (Aesculus turbinata BLUME) as an antioxidant. Decreases in the electroretinographic amplitude and outer nuclear layer thickness were suppressed by the polyphenolic compounds of the seed shells. Polyphenolic compounds from the seed shells of Japanese horse chestnut inhibited the oxidation of retinal lipids. Highly polymeric A-type proanthocyanidin from the seed shells protected the rat retina from light exposure damage by inhibiting oxidative stress and apoptotic mechanisms. PMID:29748512

  11. Protective Effect of Highly Polymeric A-Type Proanthocyanidins from Seed Shells of Japanese Horse Chestnut (Aesculus turbinata BLUME) against Light-Induced Oxidative Damage in Rat Retina.

    PubMed

    Ishihara, Tomoe; Kaidzu, Sachiko; Kimura, Hideto; Koyama, Yasurou; Matsuoka, Yotaro; Ohira, Akihiro

    2018-05-10

    Retinal tissue is exposed to oxidative stress caused by visible light. Light-damaged rat used in age-related macular degeneration (AMD) studies clarified that antioxidants decrease retinal light damage. Albino rats were exposed to 5000 Lux light for 12 h with oral administration of the polyphenolic compounds fraction (PF) from the seed shells of Japanese horse chestnut (30 mg/kg, 100 mg/kg, and 300 mg/kg body weight: BW). To evaluate the protective effects against light damage, electroretinograms (ERGs), the outer nuclear layer (ONL) thickness, the antioxidant activity of plasma, oxidized retinal lipids, and the detection of apoptosis were examined. To reveal their active compounds, PF were separated into an A-type proanthocyanidin (PAF) and a flavonol O -glycosides fraction. The protective effects of these fractions against light damage were compared by measuring the thickness of the ERGs and ONL. Compared with the negative control, the PF group (100 mg/kg and 300 mg/kg BW) significantly suppressed the decrease of the ERG amplitudes and ONL thickness. PF (300 mg/kg BW) induced the elevation of in vivo antioxidant activity, and the suppression of retinal lipid oxidation. PF administration also suppressed apoptotic cell death. The protective effects against light damage were attributable to the antioxidant activity of PAF. The light-induced damage of retinas was protected by oral administration of PF and PAF. Taken together, these compounds are potentially useful for the prevention of the disease caused by light exposure. The protective effects of retinal damage by light exposure were evaluated using polyphenolic compounds from the seed shells of Japanese horse chestnut ( Aesculus turbinata BLUME) as an antioxidant. Decreases in the electroretinographic amplitude and outer nuclear layer thickness were suppressed by the polyphenolic compounds of the seed shells. Polyphenolic compounds from the seed shells of Japanese horse chestnut inhibited the oxidation of retinal lipids. Highly polymeric A-type proanthocyanidin from the seed shells protected the rat retina from light exposure damage by inhibiting oxidative stress and apoptotic mechanisms.

  12. Effects of different tannin-rich extracts and rapeseed tannin monomers on methane formation and microbial protein synthesis in vitro.

    PubMed

    Wischer, G; Boguhn, J; Steingaß, H; Schollenberger, M; Rodehutscord, M

    2013-11-01

    Tannins, polyphenolic compounds found in plants, are known to complex with proteins of feed and rumen bacteria. This group of substances has the potential to reduce methane production either with or without negative effects on digestibility and microbial yield. In the first step of this study, 10 tannin-rich extracts from chestnut, mimosa, myrabolan, quebracho, sumach, tara, valonea, oak, cocoa and grape seed, and four rapeseed tannin monomers (pelargonidin, catechin, cyanidin and sinapinic acid) were used in a series of in vitro trials using the Hohenheim gas test, with grass silage as substrate. The objective was to screen the potential of various tannin-rich extracts to reduce methane production without a significant effect on total gas production (GP). Supplementation with pelargonidin and cyanidin did not reduce methane production; however, catechin and sinapinic acid reduced methane production without altering GP. All tannin-rich extracts, except for tara extract, significantly reduced methane production by 8% to 28% without altering GP. On the basis of these results, five tannin-rich extracts were selected and further investigated in a second step using a Rusitec system. Each tannin-rich extract (1.5 g) was supplemented to grass silage (15 g). In this experiment, nutrient degradation, microbial protein synthesis and volatile fatty acid production were used as additional response criteria. Chestnut extract caused the greatest reduction in methane production followed by valonea, grape seed and sumach, whereas myrabolan extract did not reduce methane production. Whereas chestnut extract reduced acetate production by 19%, supplementation with grape seed or myrabolan extract increased acetate production. However, degradation of fibre fractions was reduced in all tannin treatments. Degradation of dry matter and organic matter was also reduced by tannin supplementation, and no differences were found between the tannin-rich extracts. CP degradation and ammonia-N accumulation in the Rusitec were reduced by tannin treatment. The amount and efficiency of microbial protein synthesis were not significantly affected by tannin supplementation. The results of this study indicated that some tannin-rich extracts are able to reduce methane production without altering microbial protein synthesis. We hypothesized that chestnut and valonea extract have the greatest potential to reduce methane production without negative side effects.

  13. Environmental variation shifts the relationship between trees and scatterhoarders along the continuum from mutualism to antagonism.

    PubMed

    Sawaya, Gina M; Goldberg, Adam S; Steele, Michael A; Dalgleish, Harmony J

    2018-05-01

    The conditional mutualism between scatterhoarders and trees varies on a continuum from mutualism to antagonism and can change across time and space, and among species. We examined 4 tree species (red oak [Quercus rubra], white oak [Quercus alba], American chestnut [Castanea dentata] and hybrid chestnut [C. dentata × Castanea mollissima) across 5 sites and 3 years to quantify the variability in this conditional mutualism. We used a published model to compare the rates of seed emergence with and without burial to the probability that seeds will be cached and left uneaten by scatterhoarders to quantify variation in the conditional mutualism that can be explained by environmental variation among sites, years, species, and seed provenance within species. All species tested had increased emergence when buried. However, comparing benefits of burial to the probability of caching by scatterhoarders indicated a mutualism in red oak, while white oak was nearly always antagonistic. Chestnut was variable around the boundary between mutualism and antagonism, indicating a high degree of context dependence in the relationship with scatterhoarders. We found that different seed provenances did not vary in their potential for mutualism. Temperature did not explain microsite differences in seed emergence in any of the species tested. In hybrid chestnut only, emergence on the surface declined with soil moisture in the fall. By quantifying the variation in the conditional mutualism that was not caused by changes in scatterhoarder behavior, we show that environmental conditions and seed traits are an important and underappreciated component of the variation in the relationship between trees and scatterhoarders. © 2018 International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.

  14. L-Ascorbic acid metabolism during fruit development in an ascorbate-rich fruit crop chestnut rose (Rosa roxburghii Tratt).

    PubMed

    Huang, Ming; Xu, Qiang; Deng, Xiu-Xin

    2014-09-01

    Chestnut rose (Rosa roxburghii Tratt) is a fruit crop that contains unusually high levels of l-ascorbic acid (AsA; ∼1300 mg 100g(-1) FW). To explore the mechanisms underlying AsA metabolism, we investigated the distribution and abundance of AsA during fruit development. We also analyzed gene expression patterns, enzyme activities, and content of metabolites related to AsA biosynthesis and recycling. AsA first accumulated during late fruit development and continued to accumulate during ripening, with the highest accumulation rate near fruit maturity. The redox state of AsA in fruit was also enhanced during late fruit development, while leaf and other tissues had much lower levels of AsA and the redox state of AsA was lower. In mature fruit, AsA was mainly distributed in the cytoplasm of the mesocarp. Correlation analysis suggested that the gene expression patterns, enzyme activities, and related metabolite concentrations involved in the l-galactose pathway showed relatively high correlations with the accumulation rate of AsA. The gene expression pattern and activity of dehydroascorbate reductase (DHAR, EC 1.8.5.1) correlated strongly with AsA concentration, possibly indicating the crucial role of DHAR in the accumulation of high levels of AsA in chestnut rose fruit. Over expression of DHAR in Arabidopsis significantly increased the reduced AsA content and redox state. This was more effective than over expression of the l-galactose pathway gene GDP-d-mannose-3,5-epimerase (EC 5.1.3.18). These findings will enhance understanding of the molecular mechanisms regulating accumulation of AsA in chestnut rose. Copyright © 2014 Elsevier GmbH. All rights reserved.

  15. Color evaluation of seventeen European unifloral honey types by means of spectrophotometrically determined CIE L*Cab*h(ab)° chromaticity coordinates.

    PubMed

    Tuberoso, Carlo Ignazio Giovanni; Jerković, Igor; Sarais, Giorgia; Congiu, Francesca; Marijanović, Zvonimir; Kuś, Piotr Marek

    2014-02-15

    CIE (Commission Internationale de l'Eclairage) L(*)Cab(*)h(ab)° color coordinates for 305 samples of 17 unifloral honeys types (asphodel, buckwheat, black locust, sweet chestnut, citrus, eucalyptus, Garland thorn, honeydew, heather, lime, mint, rapeseed, sage, strawberry tree, sulla flower, savory and thistle) from different geographic locations in Europe were spectrophotometrically assessed and statistically evaluated. Preliminary separation of unifloral honeys was obtained by means of L(*)-C(ab)(*) color coordination correlation. Hierarchical Cluster Analysis (HCA) revealed an expected segregation of the honeys types according to their chromatic characteristics. Principal Component Analysis (PCA) allowed to obtain a more defined distinction of the 17 unifloral honey types, particularly when using 3D graphics. CIE L(*)C(ab)(*)hab(*) color coordinates were useful for the identification of several honey types. The proposed method represents a simple and efficient procedure that can be used as a basis for the authentication of unifloral honeys worldwide. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Preparation and CO 2 adsorption properties of soft-templated mesoporous carbons derived from chestnut tannin precursors

    DOE PAGES

    Nelson, Kimberly M.; Mahurin, Shannon Mark; Mayes, Richard T.; ...

    2015-10-09

    This paper presents a soft templating approach for mesoporous carbon using the polyphenolic heterogeneous biomass, chestnut tannin, as the carbon precursor. By varying synthesis parameters such as tannin:surfactant ratio, cross-linker, reaction time and acid catalyst, the pore structure could be controllably modulated from lamellar to a more ordered hexagonal array. Carbonization at 600 °C under nitrogen produced a bimodal micro-mesoporous carbonaceous material exhibiting enhanced hydrogen bonding with the soft template, similar to that shown by soft-templating of phenolic-formaldehyde resins, allowing for a tailorable pore size. By utilizing the acidic nature of chestnut tannin (i.e. gallic and ellagic acid), hexagonal-type mesostructuresmore » were formed without the use of an acid catalyst. The porous carbon materials were activated with ammonia to increase the available surface area and incorporate nitrogen-containing functionality which led to a maximum CO 2 adsorption capacity at 1 bar of 3.44 mmol/g and 2.27 mmol/g at 0 °C and 25 °C, respectively. The ammonia-activated carbon exhibited multiple peaks in the adsorption energy distribution which indicates heterogeneity of adsorption sites for CO 2 capture.« less

  17. The effects of flooding and sedimentation on seed germination of two bottomland hardwood tree species

    USGS Publications Warehouse

    Pierce, Aaron R.; King, S.L.

    2007-01-01

    Flooding and sedimentation are two of the dominant disturbances that influence tree species composition and succession in floodplain forests. The importance of these disturbances may be most notable during the germination and establishment phases of plant succession. Channelization of most alluvial systems in the southeastern United States has caused dramatic and systematic alterations to both hydrologic and sedimentation processes of floodplain systems. We determined the influence of these altered abiotic processes on the germination and growth of two common floodplain tree species: swamp chestnut oak (Quercus michauxii Nutt.) and overcup oak (Q. lyrata Walt.). Flood durations of 0 days, 15 days, and 30 days prior to germination was a factor in germination, but the effect varied by species. For instance, ovcrcup oak, which has a higher tolerance to flooding than swamp chestnut oak, had higher germination rates in the flooded treatments (15-day x?? = 78% and 30-day x?? = 85%) compared to the non-flooded treatment (x?? = 54%). In contrast, germination rates of swamp chestnut oak were negatively affected by the 30-day flood treatment. Sediment deposition rates of 2 cm of top soil, 2 cm of sand, and 8 cm of sand also affected germination, but were secondary to flood duration. The main effect of the sediment treatment in this experiment was a reduction in above-ground height of seedlings. Our study provides evidence for the importance of both flooding and sedimentation in determining tree species composition in floodplain systems, and that tolerance levels to such stressors vary by species. ?? 2007, The Society of Wetland Scientists.

  18. Osmotic dehydration effects on major and minor components of chestnut (Castanea sativa Mill.) slices.

    PubMed

    Delgado, Teresa; Pereira, José Alberto; Ramalhosa, Elsa; Casal, Susana

    2017-08-01

    The effect of osmotic dehydration (OD) conditions (temperature, time and sucrose concentration) on some nutritional parameters, soluble sugars, organic acids, fatty acids and vitamin E composition of chestnut slices was studied. Temperature at 60 °C and contact time of 7.5 h decreased significantly both protein (in 20 and 15%) and fat (in 25 and 20%) contents when compared to 30 °C and contact time of 2.5 h, simultaneously with the incorporation of sugars from the osmotic medium. An increase in temperature from 30 to 60 °C and contact time from 2.5 to 7.5 h also changed amylose percentage from 12 to 17 g/100 g of starch, suggesting modifications on starch conformation. Concerning organic acids, an increase in temperature from 30 to 60 °C induced thermal degradation of citric (54% of loss), malic (36% of loss) and ascorbic (23% of loss) acids. Temperature and sugar concentration did not affect significantly fat composition, particularly PUFA, the main fatty acid class, while contact times of 7.5 h led to the partial oxidation of linolenic acid (17% of loss when compared to 2.5 h). A 50% decrease was also observed on vitamin E content when temperature increased from 30 to 60 °C. Thus, OD might cause changes on the chemical composition of chestnut slices, requiring low temperature and contact times to avoid loss of important bioactive components such as ω-3 fatty acids (ex. linolenic acid) and vitamin E.

  19. Phylogeny of Castanea (Fagaceae) based on chloroplast trnT-L-F sequence data

    Treesearch

    Ping Lang; Fenny Dane; Thomas L. Kubisiak

    2005-01-01

    Species in the genus Castanea are widely distributed in the deciduous forests of the Northern Hemisphere from Asia to Europe and North America. They show floristic similarity but differences in chestnut blight resistance especially among eastern Asian and eastern North American species. Phylogenetic analyses were conducted in this study using...

  20. Influence of topographic aspect, precipitation and drought on radial growth of four major tree species in an Appalachian watershed

    Treesearch

    Desta Fekedulegn; Ray R., Jr. Hicks; J.J. Colbert

    2003-01-01

    This study used dated and measured tree-ring data to examine relationships between radial growth, topographic aspect, and precipitation for four hardwood species, yellow-poplar (Liriodendron tulipifera L.), northern red oak ( L.), chestnut oak (Quercus prinus L.), and red maple (Acer rubum...

  1. Abundance and frequency of the Asiatic oak weevil (Coleoptera: Curculionidae) and defoliation on American, Chinese, and hybrid chestnut ( Castanea )

    Treesearch

    Ashley E. Case; Albert (Bud) Mayfield; Stacy L. Clark; Scott E. Schlarbaum; Barbara C. Reynolds

    2016-01-01

    The Asiatic oak weevil, Cyrtepistomus castaneus Roelofs (Coleoptera: Curculionidae), is a nonnative defoliator of trees in the Fagaceae family in the United States but has not been studied on Castanea species in the southern Appalachian Mountains. Planted trees of Castanea dentata (...

  2. Dietary CP and Tannin Extracts Impact Ammonia Emissions From Manure Deposited On Dairy Barn Floors

    USDA-ARS?s Scientific Manuscript database

    The impact of dietary CP and Quebracho-Chestnut tannin extracts on dairy cow performance and N partitioning are reported elsewhere at this meeting. Mixtures of feces/urine from these studies were applied to lab-scale ventilated chambers to measure ammonia-N emissions (ANE) from simulated concrete ba...

  3. Changes in the relationship between annual tree growth and climatic variables for four hardwood species

    Treesearch

    E.R. Smith; J.C. Rennie

    1991-01-01

    A study was conducted to characterize temporal and spatial variability in the growth response of four major hardwood species (white oak, chestnut oak, northern red oak, and yellow-poplar) to climatic fluctuations, and to evaluate the role of environmental factors associated with difference in response among individuals. The study incorporated tree-ring data collected...

  4. Activated microporous-mesoporous carbon derived from chestnut shell as a sustainable anode material for high performance microbial fuel cells.

    PubMed

    Chen, Qin; Pu, Wenhong; Hou, Huijie; Hu, Jingping; Liu, Bingchuan; Li, Jianfeng; Cheng, Kai; Huang, Long; Yuan, Xiqing; Yang, Changzhu; Yang, Jiakuan

    2018-02-01

    Microbial fuel cells (MFCs) are promising biotechnologies tool to harvest electricity by decomposing organic matter in waste water, and the anode material is a critical factor in determining the performance of MFCs. In this study, chestnut shell is proposed as a novel anode material with mesoporous and microporous structure prepared via a simple carbonization procedure followed by an activation process. The chemical activation process successfully modified the macroporous structure, created more mesoporous and microporous structure and decreased the O-content and pyridinic/pyrrolic N groups on the biomass anode, which were beneficial for improving charge transfer efficiency between the anode surface and microbial biofilm. The MFC with activated biomass anode achieved a maximum power density (23.6 W m -3 ) 2.3 times higher than carbon cloth anode (10.4 W m -3 ). This study introduces a promising and feasible strategy for the fabrication of high performance anodes for MFCs derived from cost-effective, sustainable natural materials. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Grindability and combustion behavior of coal and torrefied biomass blends.

    PubMed

    Gil, M V; García, R; Pevida, C; Rubiera, F

    2015-09-01

    Biomass samples (pine, black poplar and chestnut woodchips) were torrefied to improve their grindability before being combusted in blends with coal. Torrefaction temperatures between 240 and 300 °C and residence times between 11 and 43 min were studied. The grindability of the torrefied biomass, evaluated from the particle size distribution of the ground sample, significantly improved compared to raw biomass. Higher temperatures increased the proportion of smaller-sized particles after grinding. Torrefied chestnut woodchips (280 °C, 22 min) showed the best grinding properties. This sample was blended with coal (5-55 wt.% biomass). The addition of torrefied biomass to coal up to 15 wt.% did not significantly increase the proportion of large-sized particles after grinding. No relevant differences in the burnout value were detected between the coal and coal/torrefied biomass blends due to the high reactivity of the coal. NO and SO2 emissions decreased as the percentage of torrefied biomass in the blend with coal increased. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. The effects of short- and long-term air pollutants on plant phenology and leaf characteristics.

    PubMed

    Jochner, Susanne; Markevych, Iana; Beck, Isabelle; Traidl-Hoffmann, Claudia; Heinrich, Joachim; Menzel, Annette

    2015-11-01

    Pollution adversely affects vegetation; however, its impact on phenology and leaf morphology is not satisfactorily understood yet. We analyzed associations between pollutants and phenological data of birch, hazel and horse chestnut in Munich (2010) along with the suitability of leaf morphological parameters of birch for monitoring air pollution using two datasets: cumulated atmospheric concentrations of nitrogen dioxide and ozone derived from passive sampling (short-term exposure) and pollutant information derived from Land Use Regression models (long-term exposure). Partial correlations and stepwise regressions revealed that increased ozone (birch, horse chestnut), NO2, NOx and PM levels (hazel) were significantly related to delays in phenology. Correlations were especially high when rural sites were excluded suggesting a better estimation of long-term within-city pollution. In situ measurements of foliar characteristics of birch were not suitable for bio-monitoring pollution. Inconsistencies between long- and short-term exposure effects suggest some caution when interpreting short-term data collected within field studies. Copyright © 2015 Elsevier Ltd. All rights reserved.

  7. Isolation and Characterization of Esters of Indole-3-Acetic Acid from the Liquid Endosperm of the Horse Chestnut (Aesculus species) 1

    PubMed Central

    Domagalski, Wojciech; Schulze, Aga; Bandurski, Robert S.

    1987-01-01

    Esters of indole-3-acetic acid were extracted and purified from the liquid endosperm of immature fruits of various species of the horse chestnut (Aesculus parviflora, A. baumanni, A.pavia rubra, and A. pavia humulis). The liquid endosperm contained, at least 12 chromatographically distinct esters. One of these compounds was purified and characterized as an ester of indole-3-acetic acid and myo-inositol. A second compound was found to be an ester of indole-3-acetic acid and the disaccharide rutinose (glucosyl-rhamnose). A third compound was partially characterized as an ester of indole-3-acetic acid and a desoxyaminohexose. PMID:11539676

  8. Isolation and characterization of esters of indole-3-acetic acid from the liquid endosperm of the horse chestnut (Aesculus species)

    NASA Technical Reports Server (NTRS)

    Domagalski, W.; Schulze, A.; Bandurski, R. S.

    1987-01-01

    Esters of indole-3-acetic acid were extracted and purified from the liquid endosperm of immature fruits of various species of the horse chestnut (Aesculus parviflora, A. baumanni, A. pavia rubra, and A. pavia humulis). The liquid endosperm contained, at least 12 chromatographically distinct esters. One of these compounds was purified and characterized as an ester of indole-3-acetic acid and myo-inositol. A second compound was found to be an ester of indole-3-acetic acid and the disaccharide rutinose (glucosyl-rhamnose). A third compound was partially characterized as an ester of indole-3-acetic acid and a desoxyaminohexose.

  9. Effects of feed-supplementation and hide-spray application of two sources of tannins on enteric and hide bacteria of feedlot cattle

    USDA-ARS?s Scientific Manuscript database

    Alternative pre-harvest interventions have to be evaluated to prevent carcass contamination at the slaughter house. The objectives of this study were to examine the antimicrobial effects of hydrolysable tannin-rich chestnut and condensed tannin-rich mimosa extracts on bacterial indicators of foodbo...

  10. Individual variation in acorn production by five species of southern Appalachian oaks

    Treesearch

    Cathryn H. Greenberg

    2000-01-01

    Acorns are an important wildlife food resource and seed source for oak regeneration. Most acorn production studies note wide and consistent differences in acorn productivity among individuals, but none clearly demonstrate determinants of productivity. Acorn production by black, northern red, scarlet, chestnut, and white oak was measured from 1993 to 1997 in the...

  11. Proposed modifications to the RCRA post-closure permit for the Chestnut Ridge Hydrogeologic Regime at the U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1997-05-01

    This report presents proposed modifications to several conditions of the Resource Conservation and Recovery Act (RCRA) Post-Closure Permit (PCP) for the Chestnut Ridge Hydrogeologic Regime (CRHR) (permit number TNHW-088, EPA ID No. TN3 89 009 0001). These permit conditions define the requirements for RCRA post-closure detection groundwater monitoring at the Chestnut Ridge Sediment Disposal Basin (CRSDB) and Kerr Hollow Quarry (KHQ), and RCRA post-closure corrective action groundwater monitoring at the Chestnut Ridge Security Pits (CRSPs). Modification of these PCP conditions is requested to: (1) clarify the planned integration of RCRA post-closure corrective action groundwater monitoring at the CRSPs with themore » monitoring program to be established in the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) record of decision (ROD), (2) revise several of the current technical requirements for groundwater monitoring based on implementation of the RCRA monitoring programs during 1996, (3) replace several of the technical procedures included in the PCP with updated versions recently issued by the Y-12 Plant Groundwater Protection Program (GWPP), and (4) correct inaccurate regulatory citations and references to permit conditions and permit attachments. With these modifications, the Y- 12 Plant will continue to meet the full intent of all regulatory obligations for post-closure care of these facilities. Section 2 provides the technical justification for each proposed permit modification. Section 3.0 contains proposed changes to Section II of the PCP. Modifications to site-specific permit conditions are presented in Section 4.0 (CRSDB), Section 5.0 (CRSPs), and Section 6.0 (KHQ). Sections 7.0 and 8.0 reference updated and revised procedures for groundwater sampling, and monitoring well plugging and abandonment, respectively. Appendix A includes all proposed revisions to the permit attachments.« less

  12. Calendar Year 2007 Resource Conservation and Recovery Act Annual Monitoring Report for the U.S. Department of Energy Y-12 National Security Complex, Oak Ridge, Tennessee - RCRA Post-Closure Permit Nos. TNHW-113, TNHW-116, and TNHW-128

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Elvado Environmental

    2008-02-01

    This report contains groundwater quality monitoring data obtained during calendar year (CY) 2007 at the following hazardous waste treatment, storage, and disposal (TSD) units located at the US Department of Energy (DOE) Y-12 National Security Complex (hereafter referenced as Y-12) in Oak Ridge, Tennessee; this S-3 Site, Oil Landfarm, Bear Creek Burial Grounds/Walk-In Pits (BCBG/WIP), Eastern S-3 Site Plume, Chestnut Ridge Security Pits (CRSP), Chestnut Ridge Sediment Disposal Baste (CRSDB), few Hollow Quarry (KHQ), and East Chestnut Ridge Waste Pile (ECRWP). Hit monitoring data were obtained in accordance with the applicable Resource Conservation and Recovery Act of 1976 (RCRA) hazardousmore » waste post-closure permit (PCP). The Tennessee Department of Environment and Conservation (TDEC) - Division of Solid Waste Management issued the PCPs to define the requirements for RCRA post-closure inspection, maintenance, and groundwater monitoring at the specified TSD units located within the Bear Creek Hydrogeologic Regime (PCP no. TNHW-116), Upper East Fork Poplar Creek Hydrogeologic Regime (PCP no. TNHW-113), and Chestnut Ridge Hydrogeologic Regime (PCP no. TNHW-128). Each PCP requires the Submittal of an annual RCRA groundwater monitoring report containing the groundwater sampling information and analytical results obtained at each applicable TSD unit during the preceding CY, along with an evaluation of groundwater low rates and directions and the analytical results for specified RCRA groundwater target compounds; this report is the RCRA annual groundwater monitoring report for CY 2007. The RCRA post-closure groundwater monitoring requirements specified in the above-referenced PCP for the Chestnut Ridge Regime replace those defined in the previous PCP (permit no. TNHW-088), which expired on September 18, 2005, but remained effective until the TDEC issued the new PCP in September 2006. The new PCP defines site-specific groundwater sampling and analysis requirements for the CRSDB, CRSP, and KHQ that differ from those established under the expired PCP, including modified suites of laboratory analytes (RCRA groundwater target compounds) for each site and annual rather than semiannual sampling frequencies for the CRSDB and KHQ. The new PCP also specifies the RCRA post-closure groundwater monitoring requirements for the ECRWP, a closed TSD unit that was not addressed in the expired PCP.« less

  13. Composting of pig manure and forest green waste amended with industrial sludge.

    PubMed

    Arias, O; Viña, S; Uzal, M; Soto, M

    2017-05-15

    The aim of this research was to study the composting of chestnut forest green waste (FGW) from short rotation chestnut stands amended with sludge resulting from the manufacture of Medium Density Fibreboard (MDFS) and pig manure (PM). Both FGW and MDFS presented low biodegradation potential but different characteristics in granulometry and bulk density that make its mixture of interest to achieve high composting temperatures. PM decreased the C/N ratio of the mixture and increased its moisture content (MC). Three mixtures of MDFS:FGW at volume ratios of 1:1.3 (M2), 1:2.4 (M3) and 0:1 (M4) were composted after increasing its MC to about 70% with PM. A control with food waste (OFW) and FGW (1:2.4 in volume) (M1) was run in parallel. Watering ratios reached 0.25 (M1), 1.08 (M2) 1.56 (M3) and 4.35 (M4) L PM/kg TS of added solids wastes. Treatments M2 and M3 reached a thermophilic phase shorter than M1, whilst M4 remained in the mesophilic range. After 48days of composting, temperature gradients in respect to ambient temperature were reduced, but the mineralization process continued for around 8months. Final reduction in total organic carbon reached 35-56%, depending mainly on the content in MDFS. MDFS addition to composting matrices largely reduced nitrogen losses, which range from 22% (M2) to 37% (M3) and 53% (M4). Final products had high nutrient content, low electrical conductivity and low heavy metal content which make it a valuable product for soil fertilization, right to amend in the chestnut forests and as a pillar of their sustainable management. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Mitochondrial and microsatellite DNA markers reveal a Balkan origin for the highly invasive horse-chestnut leaf miner Cameraria ohridella (Lepidoptera, Gracillariidae).

    PubMed

    Valade, R; Kenis, M; Hernandez-Lopez, A; Augustin, S; Mari Mena, N; Magnoux, E; Rougerie, R; Lakatos, F; Roques, A; Lopez-Vaamonde, C

    2009-08-01

    Biological invasions usually start with a small number of founder individuals. These founders are likely to represent a small fraction of the total genetic diversity found in the source population. Our study set out to trace genetically the geographical origin of the horse-chestnut leafminer, Cameraria ohridella, an invasive microlepidopteran whose area of origin is still unkown. Since its discovery in Macedonia 25 years ago, this insect has experienced an explosive westward range expansion, progressively colonizing all of Central and Western Europe. We used cytochrome oxidase I sequences (DNA barcode fragment) and a set of six polymorphic microsatellites to assess the genetic variability of C. ohridella populations, and to test the hypothesis that C. ohridella derives from the southern Balkans (Albania, Macedonia and Greece). Analysis of mtDNA of 486 individuals from 88 localities allowed us to identify 25 geographically structured haplotypes. In addition, 480 individuals from 16 populations from Europe and the southern Balkans were genotyped for 6 polymorphic microsatellite loci. High haplotype diversity and low measures of nucleotide diversities including a significantly negative Tajima's D indicate that C. ohridella has experienced rapid population expansion during its dispersal across Europe. Both mtDNA and microsatellites show a reduction in genetic diversity of C. ohridella populations sampled from artificial habitats (e.g. planted trees in public parks, gardens, along roads in urban or sub-urban areas) across Europe compared with C. ohridella sampled in natural stands of horse-chestnuts in the southern Balkans. These findings suggest that European populations of C. ohridella may indeed derive from the southern Balkans.

  15. Proximate analysis and physico-chemical properties of flour from the seeds of the China chestnut, Sterculia monosperma Ventenat.

    PubMed

    Noitang, S; Sooksai, S A; Foophow, T; Petsom, A

    2009-10-01

    The aim of this study was to evaluate nutritional composition of China chestnut seeds, Sterculia monosperma Vent. and analyze the physico-chemical properties of flour from the seeds. The results obtained on proximate analysis of China chestnut seeds, S. monosperma, revealed that they contained mostly carbohydrate (73.7% dm), followed by fat (12.0% dm), protein (7.8% dm), fiber (5.5% dm) and ash (1.0% dm). They have a relatively high content of potassium (12.3 mg g(-1) dm) following by phosphorus (2.30 mg g(-1) dm), magnesium (1.87 mg g(-1) dm), sulfur (0.88 mg g(-1) dm) and calcium (0.14 mg g(-1) dm). The fatty acids profile was found to be composed of mainly palmitic (42%) and oleic acids (34%), with general long-chain fatty acids the other significant component by mass (13%). Glutamic acid (17.4%), aspartic acid (12.5%) and arginine (12.5%) were the three major amino acid constituents. The purified seed starch was investigated for its morphological, starch content and physico-chemical properties, such as amylose content, swelling power, solubility and pasting properties. The starch granules were quite round, about 10-15 micron diameter and composed of more than 35% (w/w) of amylose. The pasting properties of flour from the seeds of S. monosperma revealed that gelatinization began at 72.6-73.2 degrees C and the maximum viscosity in the holding period at 95 degrees C was 633 BU. Interestingly and potentially of use, was that the viscosity at the cooling period was more than two-fold higher than that in the holding period.

  16. Phenolic compounds in chestnut (Castanea sativa Mill.) heartwood. Effect of toasting at cooperage.

    PubMed

    Sanz, Miriam; Cadahía, Estrella; Esteruelas, Enrique; Muñoz, Angel Ma; Fernández de Simón, Brígida; Hernández, Teresa; Estrella, Isabel

    2010-09-08

    The phenolic and tannic composition of heartwood extracts from Castanea sativa Mill., before and after toasting in cooperage, were studied using HPLC-DAD and HPLC-DAD/ESI-MS, and some low molecular weight phenolic compounds and hydrolyzable tannins were found. The low molecular weight phenolic compounds were lignin constituents as the acids gallic, protocatechuic, vanillic, syringic, ferulic, and ellagic, the aldehydes protocatechuic, vanillic, syringic, coniferylic, and sinapic, and the coumarin scopoletin. Their patterns were somewhat different those of oak because oak does not contain compounds such protocatechuic acid and aldehyde and is composed of much lower amounts of gallic acid than chestnut. Vescalagin and castalagin were the main ellagitannins, and acutissimin was tentatively identified for the first time in this wood. Moreover, some gallotannins were tentatively identified, including different isomers of di, tri, tetra, and pentagalloyl glucopyranose, and di and trigalloyl-hexahydroxydiphenoyl glucopyranose, comprising 20 different compounds, as well as some ellagic derivatives such as ellagic acid deoxyhexose, ellagic acid dimer dehydrated, and valoneic acid dilactone. These ellagic derivatives as well as some galloyl and hexahydroxydiphenoyl derivatives were tentatively identified for the first time in this wood. The profile of tannins was therefore different from that of oak wood because oak only contains tannins of the ellagitannins type. Seasoned and toasted chestnut wood showed a very different balance between lignin derivatives and tannins because toasting resulted in the degradation of tannins and the formation of low molecular weight phenolic compounds from lignin degradation. Moreover, the different toasting levels provoked different balances between tannins and lignin constituents because the intensity of lignin and tannin degradation was in relation to the intensity of toasting.

  17. Digestive enzymes activity in subsequent generations of Cameraria ohridella larvae harvested from horse chestnut trees after treatment with imidacloprid.

    PubMed

    Stygar, Dominika; Michalczyk, Katarzyna; Dolezych, Bogdan; Nakonieczny, Miroslaw; Migula, Pawel; Zaak, Maria; Sawczyn, Tomasz; Karcz-Socha, Iwona; Kukla, Michal; Zwirska-Korczala, Krystyna; Buldak, Rafal

    2013-01-01

    In the present study we describe the effect of chloronicotinoid pesticide (imidacloprid) on the digestive enzymes activity of the Cameraria ohridella larvae after lasting 1 year sublethal exposure to imidacloprid pesticide. Caterpillars - L4 stage (fourth instar, hyperphagic tissue-feeding phase) - were collected from chemically protected white horse chestnut trees 1 year after imidacloprid treatment, and compared with caterpillars collected from non-treated trees in a previous study. Enzymes activity of α-amylase, disaccharidases, glycosidases and proteases was assayed. The presence of pesticide in ingested food changed the digestive enzymes profile of caterpillars. The analysis of correlations between different digestive enzymes showed many significant correlations (P<0.05) among glycolytic activities like β-glucosidase and α-galactosidase activities. Statistically significant correlations for proteolytic activity were found between trypsin and chymotrypsin activity and aminopeptidase activity that occurred only in the 1st generation. PCA distinguished five primary components with eigenvalues higher than 1, from which the first two explain almost 59% of analyzed results. Surprisingly, in the pesticide treated groups significantly higher activities of sucrase and lactase in relation to control were found. In general, glycosidase (α-glucosidase, β-glucosidase and β-galactosidase) activities showed a similar pattern of activity in different generations. These results contrast with those obtained with control larvae, where significant differences in activities of α-glucosidase, β-glucosidase and β-galactosidase may result from the different quantity and quality food intake by subsequent generations of larvae. No inter-generation differences in total proteolytic activity were observed in treated larvae. The absolute value of total proteolytic activity was higher than that in the control group. The pesticide present in the vascular system of the horse chestnut tree significantly affected some of the digestive enzymes activities and - in consequence - also interrelationships between enzymes, what may affect the food digestion. Copyright © 2012 Elsevier Inc. All rights reserved.

  18. Transcriptome Analysis of Gene Expression during Chinese Water Chestnut Storage Organ Formation

    PubMed Central

    Chen, Sainan; Wang, Yan; Yu, Meizhen; Chen, Xuehao; Li, Liangjun; Yin, Jingjing

    2016-01-01

    The product organ (storage organ; corm) of the Chinese water chestnut has become a very popular food in Asian countries because of its unique nutritional value. Corm formation is a complex biological process, and extensive whole genome analysis of transcripts during corm development has not been carried out. In this study, four corm libraries at different developmental stages were constructed, and gene expression was identified using a high-throughput tag sequencing technique. Approximately 4.9 million tags were sequenced, and 4,371,386, 4,372,602, 4,782,494, and 5,276,540 clean tags, including 119,676, 110,701, 100,089, and 101,239 distinct tags, respectively, were obtained after removal of low-quality tags from each library. More than 39% of the distinct tags were unambiguous and could be mapped to reference genes, while 40% were unambiguous tag-mapped genes. After mapping their functions in existing databases, a total of 11,592, 10,949, 10,585, and 7,111 genes were annotated from the B1, B2, B3, and B4 libraries, respectively. Analysis of the differentially expressed genes (DEGs) in B1/B2, B2/B3, and B3/B4 libraries showed that most of the DEGs at the B1/B2 stages were involved in carbohydrate and hormone metabolism, while the majority of DEGs were involved in energy metabolism and carbohydrate metabolism at the B2/B3 and B3/B4 stages. All of the upregulated transcription factors and 9 important genes related to product organ formation in the above four stages were also identified. The expression changes of nine of the identified DEGs were validated using a quantitative PCR approach. This study provides a comprehensive understanding of gene expression during corm formation in the Chinese water chestnut. PMID:27716802

  19. 40 CFR 262.10 - Purpose, scope, and applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... current hazardous waste accumulation areas Boston College, Chestnut Hill, MA 120 Chemistry, Biology..., Biology, Psychology, Anthropology, Geology and Earth Sciences, and Environmental, Coastal and Ocean...

  20. [KINETICS OF PHOTO-INDUCED FREE RADICALS IN THE HUMAN HAIR CHESTNUT COLOR AFTER SHORT PERIODS OF RED, GREEN, BLUE AND WHITE LIGHT EXPOSURE].

    PubMed

    Tskhvediani, N; Chikvaidze, E; Tsibadze, A; Kvachadze, I; Gogoladze, T; Katsitadze, A

    2016-04-01

    The aim of the study was to investigate the kinetics of photo-induced free radicals in the human hair chestnut color with short-term exposure to visible light in different frequency ranges. Studies carried out on human volunteers aged 17-21 years (n=37). Hairs of volunteers of the study were not treated with dyes and other active cosmetic preparations. Hairs bundled in a bun had a length - 1.5 cm, weight - 40 mg. At the beginning background EPR-spectrum of a sample was measured and then hairs were irradiated with visible light (blue, green, red and white) of different wavelength subsequently; exposure duration - 60 minutes; after the exposure the kinetics of photo-induced free radicals was measured within 60 minutes. The radiation source was selected LED array of the four crystals that provides a nearly monochromatic radiation spectrum having no parasitic infrared and ultraviolet radiations. The studies give a reason to assume that the impact on hairs by visible electromagnetic rays a leading factor is their frequency characteristics: on the one hand - the proximity of the blue light to ultraviolet radiation, and on the other - the red light to the infrared range.

  1. Foliar nutrient variation in four species of upland oak

    Treesearch

    L. R. Auchmoody; K. P. Hammack

    1975-01-01

    Sampling of forest tree foliage for nutrient analyses is commonly restricted to specific and often dificult-to-reach locations such as the uppermost sun leaves growing on the south side of the crown. A study of 60-year-old red, white, chestnut, and scarlet oaks growing near Parsons, West Virginia, suggests that the easier-to-reach foliage from the lower crown is...

  2. Silvicultural methods of Lymantria dispar L. management: effects on Agrilus bilineatus (Weber) populations

    Treesearch

    Rose-Marie Muzika; Andrew Liebhold; Kurt. Gottschalk

    1997-01-01

    The abundance of twolined chestnut borer, Agrilus bilineatus (Weber), adults were sampled using sticky panels over a 6-year period in a mixed hardwood forest in West Virginia. Sixteen stands (average size 10.5 ha) were used in the study; eight of these were silviculturally thinned in 1989, the remainder were uncut. During 1990 and 1991, populations...

  3. An Old Chestnut Revisited: Teachers' Opinions and Attitudes toward Grading within a Competency Based Training Framework

    ERIC Educational Resources Information Center

    Richards, James

    2014-01-01

    The purpose of this study was to explore teachers' opinions with regard to the value and process of grading within a competency based training (CBT) framework, following the introduction of a formalised grading system at a specialist Technical and Further Education centre for hospitality and tourism training The data were gathered using a 16-item…

  4. Nutritional evaluation and health promoting activities of nuts and seeds cultivated in Greece.

    PubMed

    Kalogeropoulos, Nick; Chiou, Antonia; Ioannou, Maria S; Karathanos, Vaios T

    2013-09-01

    Available data suggest that genetic as well as environmental factors may influence nuts and seeds nutrients content. In this context nuts and seeds cultivated in Greece were studied. Macronutrients content was in agreement with that from other areas. Total phenolics content was in the range of 43.0 ± 2.1-1512.7 ± 60.7 mg GAE/100 g for chestnut and walnut, respectively. Thirteen to 22 individual phenolics were identified in the studied species. Oleanolic acid was in the range of 0.10-9.03 mg/100 g. Pumpkin seeds contained the higher squalene content (71.6 mg/100 g). β-Sitosterol predominated in all samples except pumpkin seeds. Tocopherols ranged from 8.9 mg/100 g (chestnut) to 29.3 mg/100 g (almond). Nuts and seeds hydrophilic extracts at quantities corresponding to the estimated daily consumption by the Greeks succeeded in inhibiting LDL oxidation in vitro by increasing lag time 1.1-14.1 times. One serving of nuts or seeds may cover a significant fraction of health promoting microconstituents daily intake.

  5. Determination of Four Major Saponins in Skin and Endosperm of Seeds of Horse Chestnut (Aesculus Hippocastanum L.) Using High Performance Liquid Chromatography with Positive Confirmation by Thin Layer Chromatography.

    PubMed

    Abudayeh, Zead Helmi Mahmoud; Al Azzam, Khaldun Mohammad; Naddaf, Ahmad; Karpiuk, Uliana Vladimirovna; Kislichenko, Viktoria Sergeevna

    2015-11-01

    To separate and quantify four major saponins in the extracts of the skin and the endosperm of seeds of horse chestnut (Aesculus hippocastanum L.) using ultrasonic solvent extraction followed by a high performance liquid chromatography-diode array detector (HPLC-DAD) with positive confirmation by thin layer chromatography (TLC). The saponins: escin Ia, escin Ib, isoescin Ia and isoescin Ib were extracted using ultrasonic extraction method. The optimized extraction conditions were: 70% methanol as extraction solvent, 80 °C as extraction temperature, and the extraction time was achieved in 4 hours. The HPLC conditions used: Zorbax SB-ODS-(150 mm × 2.1 mm, 3 μm) column, acetonitrile and 0.10% phosphoric acid solution (39:61 v/v) as mobile phase, flow rate was 0.5 mL min(-1) at 210 nm and 230 nm detection. The injection volume was 10 μL, and the separation was carried out isothermally at 30 °C in a heated chamber. The results indicated that the developed HPLC method is simple, sensitive and reliable. Moreover, the content of escins in seeds decreased by more than 30% in endosperm and by more than 40% in skin upon storage for two years. This assay can be readily utilized as a quality control method for horse chestnut and other related medicinal plants.

  6. Biochemical basis of osmotic potential for several hardwood species

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shirshac, T.L.; Gebre, G.M.; Tschaplinski, T.J.

    1995-06-01

    Metabolite concentrations in leaves of several tree species in an upland oak forest were characterized to determine the biochemical basis of species differences in osmotic potential at saturation. Hydrologic treatments at the site included ambient, wet (+33% throughfall precipitation), and dry (-33% throughfall precipitation). All samples collected from the ambient plot in May have been analyzed for free primary amino acids, inorganic ions, soluble carbohydrates, phenolic compounds, and organic acids. In all species, free primary amino acids accounted for less than 1% of the total solute pool. Dogwood had the lowest total solute concentration (687 {mu}mol/g dw), corresponding to themore » highest osmotic potential at saturation (-1.05 MPa). Understory red maple, sugar maple, scarlet oak, white oak, and overstory red maple had increasing total solute concentrations, with chestnut oak having the highest solute concentration (1126 {mu}mol/g dw) and the lowest osmotic potential (-1.76 MPa). Osmotic adjustment to water stress was evident in chestnut oak in the dry treatment throughout the growing season--up to a 0.21 MPa difference in the dry plot relative to the ambient plot in September. The data on carbohydrate concentrations, however, only partially account for the adjustments. Given that inorganic ions constituted almost 50% of the total solute pool in chestnut oak in May, any large solute adjustment would likely result from changes in inorganic ions.« less

  7. Effects of chestnut tannins on the meat quality, welfare, and antioxidant status of heat-stressed lambs.

    PubMed

    Liu, Huawei; Li, Ke; Mingbin, Lv; Zhao, Jinshan; Xiong, Benhai

    2016-06-01

    A study was conducted to evaluate the effects of chestnut tannins (CT) on the meat quality, welfare and antioxidant status of heat-stressed lambs. Lambs in one group were raised at 20°C and fed a basal diet (N), and three other groups (32°C) were fed a basal diet with 0 (CT0), 5 (CT5), and 10 g (CT10) of CT/kg. Addition of CT increased the b* and L* values of meat and superoxide dismutase and glutathione peroxidase activity in the serum and liver of heat-stressed lambs. The malondialdehyde concentration in meat, serum, and liver of heat-stressed lambs was decreased by dietary CT supplementation. Lambs in the CT0 group had higher cortisol, T3, and T4 levels, creatine kinase activity, white blood cell count, neutrophil count, neutrophil:lymphocyte ratio and a lower lymphocyte count than that in the N and CT10 groups. In conclusion, the addition of CT improved meat quality, certain stress parameters, and the antioxidant status of heat-stressed lambs. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Hydrothermal treatment of chestnut shells (Castanea sativa) to produce oligosaccharides and antioxidant compounds.

    PubMed

    Gullón, Beatriz; Eibes, Gemma; Dávila, Izaskun; Moreira, María Teresa; Labidi, Jalel; Gullón, Patricia

    2018-07-15

    Hydrothermal treatment is an environmentally friendly technology that allows the solubilisation of hemicellulosic oligosaccharides with potential for their use as prebiotics. The purpose of this study was to solubilize oligosaccharides and antioxidant compounds from chestnut shells by a hydrothermal processing. The highest content of oligosaccharides (18.3 g/L), with a relatively low level of monosaccharides (2.4 g/L) and degradation products (0.5 g/L) was obtained at 180 °C (severity of 3.08). In addition, the liquors presented a high content of phenolic and flavonoid compounds with good antioxidant properties. The GC-MS revealed that the most abundant phenolic compound was pyrogallol (13.2%). The molecular weight distribution of the solubilization products showed that a 26.5% presented an apparent Mw of 6077 g/mol and a 73.5% presented an apparent Mw of 586 g/mol with a high polydispersity index. MALDI-TOF, FTIR, and TGA analyses revealed structural information of these compounds and their thermal stability. Copyright © 2018 Elsevier Ltd. All rights reserved.

  9. Bioavailability assessment of essential and toxic metals in edible nuts and seeds.

    PubMed

    Moreda-Piñeiro, Jorge; Herbello-Hermelo, Paloma; Domínguez-González, Raquel; Bermejo-Barrera, Pilar; Moreda-Piñeiro, Antonio

    2016-08-15

    Bioavailability of essential and toxic metals in edible nuts and seeds has been assessed by using an in vitro dialyzability approach. The samples studied included walnuts, Brazil nuts, Macadamia nuts, pecans, hazelnuts, chestnuts, cashews, peanuts, pistachios and seeds (almond, pine, pumpkin and sunflower). Metals were measured by inductively coupled plasma-mass spectrometry in dialyzates and also in samples after a microwave assisted acid digestion pre-treatment. Low dialyzability percentages were found for Al, Fe and Hg; moderate percentages were found for Ba, Ca, Cd, Co, Cu, K, Li, Mg, Mn, Mo, P, Pb, Se, Sr, Tl and Zn; and high dialyzability ratios were found for As, Cr and Ni. The highest dialyzability percentages were found in raw chestnuts and raw hazelnuts. Metal dialyzability was found to be negatively affected by fat content. Positive correlation was found between carbohydrate content and metal dialyzability ratios. Protein and dietary fibre content did not influence metal bioavailability. Predicted dialyzability for some metals based on fat and protein content could also be established. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Expression Profiling of Castanea Genes during Resistant and Susceptible Interactions with the Oomycete Pathogen Phytophthora cinnamomi Reveal Possible Mechanisms of Immunity

    PubMed Central

    Santos, Carmen; Duarte, Sofia; Tedesco, Sara; Fevereiro, Pedro; Costa, Rita L.

    2017-01-01

    The most dangerous pathogen affecting the production of chestnuts is Phytophthora cinnamomi a hemibiotrophic that causes root rot, also known as ink disease. Little information has been acquired in chestnut on the molecular defense strategies against this pathogen. The expression of eight candidate genes potentially involved in the defense to P. cinnamomi was quantified by digital PCR in Castanea genotypes showing different susceptibility to the pathogen. Seven of the eight candidate genes displayed differentially expressed levels depending on genotype and time-point after inoculation. Cast_Gnk2-like revealed to be the most expressed gene across all experiments and the one that best discriminates between susceptible and resistant genotypes. Our data suggest that the pre-formed defenses are crucial for the resistance of C. crenata to P. cinnamomi. A lower and delayed expression of the eight studied genes was found in the susceptible Castanea sativa, which may be related with the establishment and spread of the disease in this species. A working model integrating the obtained results is presented. PMID:28443110

  11. Changes in the biological activity of chestnut soils upon the long-term application of fertilizers in a rotation with oil-bearing crops

    NASA Astrophysics Data System (ADS)

    Eleshev, R. E.; Bakenova, Z. B.

    2012-11-01

    Experimental studies showed that irrigated chestnut soils on the piedmont of the Zailiiskiy Alatau Range are characterized by the moderate activity of the hydrolytic and redox enzymes. The use of these soils in the crop rotation system increases the hydrolytic activity of the enzymes (invertase, urease, and ATP synthase) by 30% in comparison with the monoculture; at the same time, it does not have a significant impact on the changes in the biological activity of the redox enzymes (catalase and dehydrogenase). The hydrolytic activity of the soils is activated to a greater extent in the crop rotation and in the monoculture against the background application of organic fertilizers. In this case, the recommended rates of mineral fertilizers do not inhibit the activity of the hydrolytic and redox enzymes. An increase in the hydrolytic activity of the enzymes directly affects the yield of oilseed flax. Therefore, indices of the hydrolytic activity of soils can be used as a test for the diagnostics of the efficiency of fertilizers both in crop rotation and monoculture systems.

  12. Influence of chestnut tannins on welfare, carcass characteristics, meat quality, and lipid oxidation in rabbits under high ambient temperature.

    PubMed

    Liu, Huawei; Zhou, Daowei; Tong, Jianming; Vaddella, Venkata

    2012-01-01

    A study was conducted to evaluate the influence of chestnut tannins (CT) on welfare, carcass characteristics, meat quality, and lipid oxidation in rabbits under high ambient temperature. Rabbits in one group were raised at 20°C and fed with basal diet (N) and other three groups (33°C) were fed basal diet with 0 (C), 5 (CT5), and 10 g (CT10) of CT/kg of diet. Compared with the C group, rabbits in CT10 had higher pH(24) and lower cooking loss and thiobarbituric acid reacting substance values at 0, 30, and 60 min of forced oxidation. Rabbits in C group had higher cortisol levels, creatine kinase activities, white blood cell counts, neutrophil percentage, neutrophil:lymphocyte ratio and lower T(3), T(4) levels, lymphocyte percentage than N and CT10 groups. Supplementation of CT seemed to have a positive effect on growth performance, welfare, and meat quality of rabbits under high ambient temperature. Copyright © 2011 Elsevier Ltd. All rights reserved.

  13. Comparative vesicle proteomics reveals selective regulation of protein expression in chestnut blight fungus by a hypovirus.

    PubMed

    Wang, Jinzi; Wang, Fangzhen; Feng, Youjun; Mi, Ke; Chen, Qi; Shang, Jinjie; Chen, Baoshan

    2013-01-14

    The chestnut blight fungus (Cryphonectria parasitica) and hypovirus constitute a model system to study fungal pathogenesis and mycovirus-host interaction. Knowledge in this field has been gained largely from investigations at gene transcription level so far. Here we report a systematic analysis of the vesicle proteins of the host fungus with/without hypovirus infection. Thirty-three differentially expressed protein spots were identified in the purified vesicle protein samples by two-dimensional electrophoresis and mass spectrometry. Down-regulated proteins were mostly cargo proteins involved in primary metabolism and energy generation and up-regulated proteins were mostly vesicle associated proteins and ABC transporter. A virus-encoded protein p48 was found to have four forms with different molecular mass in vesicles from the virus-infected strain. While a few of the randomly selected differentially expressed proteins were in accordance with their transcription profiles, majority were not in agreement with their mRNA accumulation patterns, suggesting that an extensive post-transcriptional regulation may have occurred in the host fungus upon a hypovirus infection. Copyright © 2012 Elsevier B.V. All rights reserved.

  14. Gymnema

    MedlinePlus

    ... combination of gymnema, hydroxycitric acid, and niacin-bound chromium by mouth can reduce body weight in people ... these products include alpha-lipoic acid, bitter melon, chromium, devil's claw, fenugreek, garlic, guar gum, horse chestnut, ...

  15. 4. PERSPECTIVE VIEW OF MAIN AND EAST ELEVATIONS, SHOWING VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. PERSPECTIVE VIEW OF MAIN AND EAST ELEVATIONS, SHOWING VIEW TOWARD CARPENTER'S HALL - Carpenters' Company, Front Store, 322 Chestnut Street & Carpenters' Court, Philadelphia, Philadelphia County, PA

  16. Dispersive Raman spectroscopy for the nondestructive and rapid assessment of honey quality

    NASA Astrophysics Data System (ADS)

    Mignani, A. G.; Ciaccheri, L.; Mencaglia, A. A.; Di Sanzo, R.; Carabetta, S.; Russo, M. T.

    2015-09-01

    Raman spectroscopy performed using optical fibers, with excitation at 1064 nm and a dispersive detection scheme, was utilized to measure a selection of unifloral honeys produced in the Italian region of Calabria. The honey samples had three different botanical origins: chestnut, citrus, and acacia, respectively. A multivariate processing of the spectroscopic data enabled us to distinguish their botanical origin, and to build predictive models for quantifying important nutraceutic indicators such as the main sugars and potassium. Furthermore, the Raman spectra of chestnut honeys were compared with the taste profile measured by an electronic tongue, and a good correlation to bitter/savory taste was obtained. This experiment indicates the excellent potentials of Raman spectroscopy as an analytical tool for the nondestructive and rapid assessment of food-quality indicators.

  17. 93. TOWER STAIRHALL, SOUTH WALL, WEST TABERNACLE FRAME. DETAIL OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    93. TOWER STAIRHALL, SOUTH WALL, WEST TABERNACLE FRAME. DETAIL OF DOG EAR AND TRUSS (BRACKET) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  18. 1. Historic American Buildings Survey, Theodore F. Dillon, Photographer December, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey, Theodore F. Dillon, Photographer December, 1959 FRONT (SOUTH) ELEVATION. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  19. Host Preferences of Gypsy Moth on a New Frontier of Infestation

    Treesearch

    David A. Gansner; Owen W. Herrick; Owen W. Herrick

    1985-01-01

    Knowing what gypsy moth likes to eat can aid forest managers in making cost-effective control decisions. A recent 5-year study of defoliation in central Pennsylvania gives an up-to-date index of host species preferences. Chestnut oak is the most preferred species. Following in relatively close order are the other oaks and aspen. Hardwood species that rank low on the...

  20. Fifty-three polymorphic microsatellite loci in the chestnut blight fungus, Cryphonectria parasitica

    Treesearch

    T.L. Kubisiak; C. Dutech; M.G. Milgroom

    2006-01-01

    We report on 53 microsatellite loci for use in population genetic or linkage mapping studies in Cryphonectria parasitica . In 40 isolates collected from throughout the Northern Hemisphere, the number of alleles per locus ranged from two to 14 (mean 5.17) with gene diversity values ranging from 0.049 to 0.859 (mean 0.437). Samples from Asia were more...

  1. The attack of Aesculus hippocastanum L. by Cameraria ohridella Deschka and Dimic (Lepidoptera: Gracillariidae) in Greece

    Treesearch

    Nikolaos Avtzis; Dimitrios Avtzis

    2003-01-01

    The horse chestnut leaf miner Cameraria ohridella is a relatively new insect in Europe, which was first observed at Lake Ohrid in 1984. Within the framework of the European Community Project "Controcam", in which Greece participates through the TEI of Kavala, Department of Forestry in Drama, we attempted for the first time to study the bio-...

  2. 20. VIEW OF ELEVATOR SHAFT FROM FIRST FLOOR TO SKYLIGHT, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. VIEW OF ELEVATOR SHAFT FROM FIRST FLOOR TO SKYLIGHT, LOOKING SKYWARD, NORTH - Bates Manufacturing Company, Storehouse, Northeast corner of Chestnut Street & Hines Alley, Lewiston, Androscoggin County, ME

  3. 7 CFR 319.56-7 - Territorial applicability and exceptions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., kale, kudzu, leek, lettuce, onions, Portuguese cabbage, turnip, udo, water chestnut, watercress... other containers made of coconut fronds are not approved for use as containers for fruits and vegetables...

  4. 7 CFR 319.56-7 - Territorial applicability and exceptions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., kale, kudzu, leek, lettuce, onions, Portuguese cabbage, turnip, udo, water chestnut, watercress... other containers made of coconut fronds are not approved for use as containers for fruits and vegetables...

  5. 7 CFR 319.56-7 - Territorial applicability and exceptions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., kale, kudzu, leek, lettuce, onions, Portuguese cabbage, turnip, udo, water chestnut, watercress... other containers made of coconut fronds are not approved for use as containers for fruits and vegetables...

  6. 7 CFR 319.56-7 - Territorial applicability and exceptions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., kale, kudzu, leek, lettuce, onions, Portuguese cabbage, turnip, udo, water chestnut, watercress... other containers made of coconut fronds are not approved for use as containers for fruits and vegetables...

  7. 8. Historic American Buildings Survey, James C. Massey, Photographer November, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Historic American Buildings Survey, James C. Massey, Photographer November, 1959 INTERIOR LOOKING TO REAR. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  8. 4. RAMP FOR BENJAMIN FRANKLIN BRIDGE (FOURTH ST.) BETWEEN VINE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. RAMP FOR BENJAMIN FRANKLIN BRIDGE (FOURTH ST.) BETWEEN VINE AND RACE STS., LOOKING NORTHWEST - Independence National Historical Park, Walnut, Sixth, Chestnut & Second Streets, Philadelphia, Philadelphia County, PA

  9. Strategies for getting through labor

    MedlinePlus

    ... best ways to prepare is to take a childbirth class to learn what to expect in labor. ... PA: Elsevier Saunders; 2012:chap 51. Minnich ME. Childbirth preparation and nonpharmacologic analgesia. In: Chestnut DH, Wong ...

  10. 7 CFR 319.56-7 - Territorial applicability and exceptions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., gingerroot, horseradish, kale, kudzu, leek, lettuce, onions, Portuguese cabbage, turnip, udo, water chestnut... other containers made of coconut fronds are not approved for use as containers for fruits and vegetables...

  11. 9. Historic American Buildings Survey, James C. Massey, Photographer November, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Historic American Buildings Survey, James C. Massey, Photographer November, 1959 DETAIL OF EXPOSED ROOF TRUSS. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  12. 7. Historic American Buildings Survey, James C. Massey, Photographer November, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic American Buildings Survey, James C. Massey, Photographer November, 1959 REAR FACADE ON RANSTEAD STREET. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  13. 6. Historic American Buildings Survey, Theodore F. Dillon, Photographer December, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Historic American Buildings Survey, Theodore F. Dillon, Photographer December, 1959 ROOF TRUSSES EXPOSED DURING DEMOLITION. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  14. 122. HISTORIC AMERICAN BUILDINGS SURVEY TEAM MEASURING EXTERIOR OF INDEPENDENCE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    122. HISTORIC AMERICAN BUILDINGS SURVEY TEAM MEASURING EXTERIOR OF INDEPENDENCE HALL (LEE NELSON ON CORNER LEANING OVER) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  15. [The validity of radioimmunologic determination of bioavailability of beta-escin in horse chestnut extracts].

    PubMed

    Schrödter, A; Loew, D; Schwankl, W; Rietbrock, N

    1998-09-01

    The bioavailability under steady state conditions of a standard, slow-release horse chestnut seed extract (HCSE)-containing product was compared with that of an analogous, fast-release test preparation (Noricaven novo) in a prospective, randomised, double-blind study in a double cross-over design. The serum concentration of beta-escin (CAS 6805-41-0) was measured by radioimmunoassay. In addition, the biopharmaceutical properties of the HCSEs present in the products were investigated, the amount and composition of the active ingredient, escin, being analysed with a validated HPLC method. The pharmacokinetics of this study were compared with the corresponding data of a similar investigation carried out under analogous conditions concerning study design, analytical methods and reference preparation. Comparison of the similar studies revealed differences in characteristic pharmakokinetic values of beta-escin in terms of a shift of the concentration time curves as could be demonstrated for the reference product. The total amounts of escin in the two products investigated did not differ significantly. However, quantitative and qualitative differences were detected in the constituents of the two different extract preparations. It is concluded that the high specificity of the validated beta-escin radioimmunoassay leads to analytical imprecision due to the variable constituents of the extract preparations used. It is necessary to test whether this problem can be solved using an analytical approach, which is specific for each extract.

  16. 5. AERIAL VIEW, FROM NORTH, SHOWING ROOF OF CARPENTER'S FRONT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. AERIAL VIEW, FROM NORTH, SHOWING ROOF OF CARPENTER'S FRONT STORE AND CARPENTER'S HALL IN CENTER BACKGROUND - Carpenters' Company, Front Store, 322 Chestnut Street & Carpenters' Court, Philadelphia, Philadelphia County, PA

  17. 3. Photocopy of drawing (from Moses King, Philadelphia and Notable ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopy of drawing (from Moses King, Philadelphia and Notable Philadelphians, 1902) MAIN BUILDING WITH ADDITION - New York Mutual Life Insurance Company Building, 1001-1005 Chestnut Street, Philadelphia, Philadelphia County, PA

  18. 52. SUPREME COURT ROOM, SOUTH WALL, WEST WINDOW DETAIL OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    52. SUPREME COURT ROOM, SOUTH WALL, WEST WINDOW DETAIL OF EAST SPLAYED JAMB AND TRIM (NOTE REPAIRED AREAS) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  19. 13. DETAIL SOUTH FACADE, ENTRANCE Copy photograph of photogrammetric plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. DETAIL- SOUTH FACADE, ENTRANCE Copy photograph of photogrammetric plate LC-HABS-GS07-1116-102R. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  20. 10. Historic American Buildings Survey, James C. Massey, Photographer November, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Historic American Buildings Survey, James C. Massey, Photographer November, 1959 DETAIL OF COLUMN CAPITAL, FRONT ALCOVE. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  1. 17. INTERIOR: SKYLIGHT FROM MAIN LOBBY Copy photograph of photogrammetric ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. INTERIOR: SKYLIGHT FROM MAIN LOBBY Copy photograph of photogrammetric plate LC-HABS-GS07-1116-111L. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  2. Agrobacterium rhizogenes-mediated DNA transfer to Aesculus hippocastanum L. and the regeneration of transformed plants.

    PubMed

    Zdravković-Korać, S; Muhovski, Y; Druart, P; Calić, D; Radojević, L

    2004-04-01

    Hairy roots were induced from androgenic embryos of horse chestnut (Aesculus hippocastanum L.) by infection with Agrobacterium rhizogenes strain A4GUS. Single roots were selected according to their morphology in the absence of antibiotic or herbicide resistance markers. Seventy-one putative transformed hairy root lines from independent transformation events were established. Regeneration was induced in MS liquid medium supplemented with 30 microM 6-benzylaminopurine (BA), and the regenerants were multiplied on MS solid medium containing 10 microM BA. Following elongation on MS medium supplemented with 1 microM BA and 500 mg/l polyvinylpyrrolidone, the shoots were subjected to a root-inducing treatment. Stable integration of TL-DNA within the horse chestnut genome was confirmed by Southern hybridization. The copy number of transgenes was estimated to be from two to four.

  3. Phylogenetic and phenotypic characterisation of Sirococcus castaneae comb. nov. (synonym Diplodina castaneae), a fungal endophyte of European chestnut.

    PubMed

    Meyer, Joana B; Trapiello, Estefanía; Senn-Irlet, Beatrice; Sieber, Thomas N; Cornejo, Carolina; Aghayeva, Dilzara; González, Ana J; Prospero, Simone

    2017-08-01

    In this paper we resolve the taxonomic status of the fungus Diplodina castaneae (Ascomycetes, Diaporthales, Gnomoniaceae) which occurs on the European chestnut (Castanea sativa) as endophyte and as the causal agent of Javart disease. Specimens from Switzerland, Spain, and Azerbaijan were sequenced at five nuclear loci (β-tubulin, EF-1α, ITS, LSU, and RPB2). Phylogenies were inferred to place D. castaneae in the Gnomoniaceae family. Moreover, growth rates and morphological characteristics on different agar media were assessed and compared to those of Gnomoniopsis castaneae, which can easily be confused with D. castaneae. Based on morphological and phylogenetic characteristics, we propose to reallocate D. castaneae to the genus Sirococcus, as S. castaneae comb. nov. Copyright © 2017 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  4. Purified phenolics from hydrothermal treatments of biomass: ability to protect sunflower bulk oil and model food emulsions from oxidation.

    PubMed

    Conde, Enma; Moure, Andrés; Domínguez, Herminia; Gordon, Michael H; Parajó, Juan Carlos

    2011-09-14

    The phenolic fractions released during hydrothermal treatment of selected feedstocks (corn cobs, eucalypt wood chips, almond shells, chestnut burs, and white grape pomace) were selectively recovered by extraction with ethyl acetate and washed with ethanol/water solutions. The crude extracts were purified by a relatively simple adsorption technique using a commercial polymeric, nonionic resin. Utilization of 96% ethanol as eluting agent resulted in 47.0-72.6% phenolic desorption, yielding refined products containing 49-60% w/w phenolics (corresponding to 30-58% enrichment with respect to the crude extracts). The refined extracts produced from grape pomace and from chestnut burs were suitable for protecting bulk oil and oil-in-water and water-in-oil emulsions. A synergistic action with bovine serum albumin in the emulsions was observed.

  5. Environmental fate of emamectin benzoate after tree micro injection of horse chestnut trees.

    PubMed

    Burkhard, Rene; Binz, Heinz; Roux, Christian A; Brunner, Matthias; Ruesch, Othmar; Wyss, Peter

    2015-02-01

    Emamectin benzoate, an insecticide derived from the avermectin family of natural products, has a unique translocation behavior in trees when applied by tree micro injection (TMI), which can result in protection from insect pests (foliar and borers) for several years. Active ingredient imported into leaves was measured at the end of season in the fallen leaves of treated horse chestnut (Aesculus hippocastanum) trees. The dissipation of emamectin benzoate in these leaves seems to be biphasic and depends on the decomposition of the leaf. In compost piles, where decomposition of leaves was fastest, a cumulative emamectin benzoate degradation half-life time of 20 d was measured. In leaves immersed in water, where decomposition was much slower, the degradation half-life time was 94 d, and in leaves left on the ground in contact with soil, where decomposition was slowest, the degradation half-life time was 212 d. The biphasic decline and the correlation with leaf decomposition might be attributed to an extensive sorption of emamectin benzoate residues to leaf macromolecules. This may also explain why earthworms ingesting leaves from injected trees take up very little emamectin benzoate and excrete it with the feces. Furthermore, no emamectin benzoate was found in water containing decomposing leaves from injected trees. It is concluded, that emamectin benzoate present in abscised leaves from horse chestnut trees injected with the insecticide is not available to nontarget organisms present in soil or water bodies. Published 2014 SETAC.

  6. Determination of Four Major Saponins in Skin and Endosperm of Seeds of Horse Chestnut (Aesculus Hippocastanum L.) Using High Performance Liquid Chromatography with Positive Confirmation by Thin Layer Chromatography

    PubMed Central

    Abudayeh, Zead Helmi Mahmoud; Al Azzam, Khaldun Mohammad; Naddaf, Ahmad; Karpiuk, Uliana Vladimirovna; Kislichenko, Viktoria Sergeevna

    2015-01-01

    Purpose: To separate and quantify four major saponins in the extracts of the skin and the endosperm of seeds of horse chestnut (Aesculus hippocastanum L.) using ultrasonic solvent extraction followed by a high performance liquid chromatography-diode array detector (HPLC-DAD) with positive confirmation by thin layer chromatography (TLC). Methods: The saponins: escin Ia, escin Ib, isoescin Ia and isoescin Ib were extracted using ultrasonic extraction method. The optimized extraction conditions were: 70% methanol as extraction solvent, 80 °C as extraction temperature, and the extraction time was achieved in 4 hours. The HPLC conditions used: Zorbax SB-ODS-(150 mm × 2.1 mm, 3 μm) column, acetonitrile and 0.10% phosphoric acid solution (39:61 v/v) as mobile phase, flow rate was 0.5 mL min−1 at 210 nm and 230 nm detection. The injection volume was 10 μL, and the separation was carried out isothermally at 30 °C in a heated chamber. Results: The results indicated that the developed HPLC method is simple, sensitive and reliable. Moreover, the content of escins in seeds decreased by more than 30% in endosperm and by more than 40% in skin upon storage for two years. Conclusion: This assay can be readily utilized as a quality control method for horse chestnut and other related medicinal plants. PMID:26819933

  7. Sugars profiles of different chestnut (Castanea sativa Mill.) and almond (Prunus dulcis) cultivars by HPLC-RI.

    PubMed

    Barreira, João C M; Pereira, José Alberto; Oliveira, M Beatriz P P; Ferreira, Isabel C F R

    2010-03-01

    Sugar profiles of different almond and chestnut cultivars were obtained by high-performance liquid chromatography (HPLC), by means of a refractive index (RI) detector. A solid-liquid extraction procedure was used in defatted and dried samples. The chromatographic separation was achieved using a Eurospher 100-5 NH(2) column using an isocratic elution with acetonitrile/water (70:30, v/v) at a flow rate of 1.0 ml/min. All the compounds were separated in 16 min. The method was optimized and proved to be reproducible and accurate. Generally, more than 95% of sugars were identified for both matrixes. Sugars profiles were quite homogeneous for almond cultivars; sucrose was the main sugar (11.46 +/- 0.14 in Marcona to 22.23 +/- 0.59 in Ferragnes g/100 g of dried weight), followed by raffinose (0.71 +/- 0.05 in Ferraduel to 2.11 +/- 0.29 in Duro Italiano), glucose (0.42 +/- 0.12 in Pegarinhos two seeded to 1.47 +/- 0.19 in Ferragnes) and fructose (0.11 +/- 0.02 in Pegarinhos two seeded to 0.59 +/- 0.05 in Gloriette). Commercial cultivars proved to have higher sucrose contents, except in the case of Marcona. Nevertheless, chestnut cultivars revealed a high heterogeneity. Sucrose was the main sugar in Aveleira (22.05 +/- 1.48), Judia (23.30 +/- 0.83) and Longal (9.56 +/- 0.91), while glucose was slightly prevalent in Boa Ventura (6.63 +/- 0.49). The observed variance could serve for inter-cultivar discrimination.

  8. [Micrococcus sp.--the pathogen of leaf necrosis of horse-chestnuts (Aesculus L.) in Kiev].

    PubMed

    Iakovleva, L M; Makhinia, L V; Shcherbina, T N; Ogorodnik, L E

    2013-01-01

    A group of phytopathogenic bacteria was isolated from patterns of drying horse-chestnuts (Aesculus L.), which grow in Kyiv. The properties of slowly growing, highly aggressive microorganisms have been described in the paper. They grow up on the 8-10th day after sowing. The investigated microorganisms form very small (0.5-1 mm in diameter) colonies on the potato agar. Bacteria are protuberant, shining, smooth with flat edges, they are pale yellow, yellow, or pink. The bacteria are Gram-positive, spherical, are disposed in smears singly, in pairs, as accumulations, or netting. They are aerobes, do not form spores, are not mobile. They are inert in respect of different sources of carbon. They reduce nitrates, do not dilute gelatin, do not hydrolyze starch, do not release hydrogen sulphide and indole. The bacteria are catalase-positive, oxidase-negative. They do not cause potato and carrot rot. They lose quickly their viability under the laboratory conditions. The saturated acids C 14:0; C 15:0; C16:0; C18:0 have been revealed in the composition of cellular fatty acids. Microorganisms are identified as Micrococcus sp. Under artificial inoculation this highly aggressive pathogen causes drying of the horse-chestnut buds and necrosis, which occupies 1/3-1/2 of the leaf plate. A wide zone of chlorosis, surrounding necrosis, may occupy the whole leaf surface. The infected leaves use to twist up from the top (apex) or along a midrib and to dry.

  9. Stump sprout growth and quality of several Appalachian hardwood species after clearcutting

    Treesearch

    G. W. Wendel

    1975-01-01

    Results of a 10-year study showed that stumps from 50- to 60-year-old red oak, black cherry, yellow-poplar, white oak, and chestnut oak trees sprouted vigorously. A high percentage of the dominant sprouts had good stem form, and many had excellent height and diameter growth. For all species, we found that the proportion of stumps sprouting, number of sprouts per stump...

  10. Determination of escin content in androgenic embryos and hairy root culture of Aesculus hippocastanum.

    PubMed

    Calić-Dragosavac, Dusica; Zdravković-Korać, Snezana; Savikin-Fodulović, Katarina; Radojević, Ljiljana; Vinterhalter, Branka

    2010-05-01

    Escin, a group of chemically related triterpenic glycosides, is widely used in commercial preparations for the treatment of venous insufficiency. Since the zygotic embryo cotyledons accumulate the highest amount of escin, it is currently extracted from the seeds of horse chestnut, Aesculus hippocastanum L. (Hippocastanaceae), on a large scale. As this material is available during only short period of the year, we studied the possibility of using plant tissue culture to obtain escin. For this purpose, the content of escin in androgenic embryos and hairy root cultures of horse chestnut was studied. Escin content was found to be dependent on the stage of androgenic embryo development and the type of phytoregulator supplemented to the nutritive medium. In the absence of phytoregulators, androgenic embryos at the globular stage of development contained approximately four times less escin than those at the cotyledonary stage. Inclusion of various phytoregulators in the nutritive media stimulated escin production. Among them, 2,4-dichlorophenoxyacetic acid (2,4-D) showed the most pronounced effect, with escin content almost reaching that found in zygotic embryos (6.77% versus 6.96%). Two hairy root clones produced substantial amounts of escin (3.57% and 4.09%), less than zygotic embryos, but higher than cotyledonary embryos on phytoregulator-free medium.

  11. Chemometric analysis of minerals in gluten-free products.

    PubMed

    Gliszczyńska-Świgło, Anna; Klimczak, Inga; Rybicka, Iga

    2018-06-01

    Numerous studies indicate mineral deficiencies in people on a gluten-free (GF) diet. These deficiencies may indicate that GF products are a less valuable source of minerals than gluten-containing products. In the study, the nutritional quality of 50 GF products is discussed taking into account the nutritional requirements for minerals expressed as percentage of recommended daily allowance (%RDA) or percentage of adequate intake (%AI) for a model celiac patient. Elements analyzed were calcium, potassium, magnesium, sodium, copper, iron, manganese, and zinc. Analysis of %RDA or %AI was performed using principal component analysis (PCA) and hierarchical cluster analysis (HCA). Using PCA, the differentiation between products based on rice, corn, potato, GF wheat starch and based on buckwheat, chickpea, millet, oats, amaranth, teff, quinoa, chestnut, and acorn was possible. In the HCA, four clusters were created. The main criterion determining the adherence of the sample to the cluster was the content of all minerals included to HCA (K, Mg, Cu, Fe, Mn); however, only the Mn content differentiated four formed groups. GF products made of buckwheat, chickpea, millet, oats, amaranth, teff, quinoa, chestnut, and acorn are better source of minerals than based on other GF raw materials, what was confirmed by PCA and HCA. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  12. Dietary Effects of Oregano (Origanum Vulgaris L.) Plant or Sweet Chestnut (Castanea Sativa Mill.) Wood Extracts on Microbiological, Chemico-Physical Characteristics and Lipid Oxidation of Cooked ham During Storage.

    PubMed

    Ranucci, David; Miraglia, Dino; Trabalza-Marinucci, Massimo; Acuti, Gabriele; Codini, Michela; Ceccarini, Maria Rachele; Forte, Claudio; Branciari, Raffaella

    2015-11-02

    The aim of this study was to evaluate the dietary effect of feeding pigs with diets enriched with sweet chestnut wood ( Castanea sativa Mill.) or oregano ( Origanum vulgaris L.) extract on the microbiological and chemical characteristics of cooked pork ham. Three groups of 10 pigs were fed with a control diet (CTRL), with the CTRL diet enriched with 0.2% of oregano extract (OR) and with the CTRL diet enriched with 0.2% of sweet chestnut wood extract (SCW), respectively. Six cooked hams per group were produced, sliced and packaged under a modified atmosphere (N2:CO2=80:20) and stored at refrigeration temperature (4±1°C). Three packages per cooked ham were sampled for analyses at three different storage times (0, 10 and 20 days). At day 0 time, antioxidant capacity of the products (ORAC FL assay) and chemical composition were performed. At each sampling time, from all the samples the following analyses were performed: total microbial count (TMC), lactic acid bacteria count (LAB), Enterobacteriaceae count, Listeria monocytogenes , pH value, colour coordinates (L*, a*, b*), total basic volatile nitrogen (TBVN) and thiobarbituric reactive substances (TBARs) determinations. No differences in TMC, LAB and Enterobacteriaceae count, pH, TBVN, chemical composition and L* values were registered between the three groups at all the sampling times considered. No Listeria monocytogenes was detected in the samples tested. Significant differences were registered for ORAC FL at 0 days, a* and b* values and TBARs value at 10 and 20 days of storage, with higher values for ORAC FL , a* and b* values and lower values for TBARs in SCW and OR than CTRL. No antimicrobial effect could be recorded for OR and SCW but a higher oxidative stability, also highlighted by the colour maintenance, was observed in both OR and SCW.

  13. Key role of hydrogen peroxide in antimicrobial activity of spring, Honeydew maquis and chestnut grove Corsican honeys on Pseudomonas aeruginosa DNA.

    PubMed

    Poli, J-P; Guinoiseau, E; Luciani, A; Yang, Y; Battesti, M-J; Paolini, J; Costa, J; Quilichini, Y; Berti, L; Lorenzi, V

    2018-05-01

    In honeys, several molecules have been known for their antibacterial or wound healing properties. Corsican honeys just began to be tested for their antimicrobial activity with promising results on Pseudomonas aeruginosa. So, identification of active molecules and their mode of action was determined. Hydrogen peroxide concentrations were evaluated and, in parallel, the minimal inhibitory concentrations (MIC) values were performed with and without catalase. More, the quantity of phenolic compounds and ORAC assay were measured. Observation of antibacterial action was done using scanning electron microscopy (SEM) followed by plasmidic DNA extraction. MIC values of chestnut grove and honeydew maquis honeys vary between 7 and 8%, showing a strong antimicrobial capacity, associated with a plasmidic DNA degradation. When catalase is added, MIC values significatively increase (25%) without damaging DNA, proving the importance of H 2 O 2 . This hypothesis is confirmed by SEM micrographies which did not show any morphological damages but a depletion in bacterial population. Although, such low concentrations of H 2 O 2 (between 23 μmol l -1 and 54 μmol l -1 ) cannot explain antimicrobial activity and might be correlated with phenolic compounds concentration. Thus, Corsican honeys seem to induce DNA damage when H 2 O 2 and phenolic compounds act in synergy by a putative pro-oxidant effect. We started to determine the antibacterial efficiency of Corsican chestnut grove and honeydew maquis honeys on Pseudomonas aeruginosa. No morphological alteration of the bacterial surface was observed. Antimicrobial action seems to be related to the synergy between hydrogen peroxide and phenolic compounds. The exerted pro-oxidant activity leads to a degradation of P. aeruginosa plasmidic DNA. This is the first study that investigate the primary antibacterial mechanism of Corsican honeys. © 2018 The Society for Applied Microbiology.

  14. A Practical Field Method of Site Evaluation for Commercially Important Southern Hardwoods

    Treesearch

    James B. Baker; W.M. Broadfoot

    1979-01-01

    A new method of evaluating sites for planted cottonwood, sweetgum, sycamore, green ash, hackberry, sugarberry, pecan, yellow poplar and Nuttall, water, willow, swamp chestnut, Shumard and cherrybark oaks is presented.

  15. Site Index Curves For Upland Oak in the Southeast

    Treesearch

    David J. Olson

    1959-01-01

    These site index curves are based on 697 observations of height on age for white, northern red, southern red, scarlet, black, and chestnut oak in the Virginia- Carolina Piedmont and the Southern Appalachian Mountains.

  16. Microencapsulation of Lithium

    DTIC Science & Technology

    1985-12-31

    nuts, chestnuts, f ilberts, hazelnuts, pecans , walnuts (all nuts, in %hells). In other countries, the appropriate government regulatory agencies...75012 Paris, France, 10 Rue Villiot .. ...... . . . . . . 347.87-45 NEAR EAST 20124 Milan, Italy , Via Rosellini 12 ... ...... ...... 688.4563 Hemel

  17. 18. INTERIOR: ARCH AND COLUMN NEAR FRONT Copy photograph of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. INTERIOR: ARCH AND COLUMN NEAR FRONT Copy photograph of photogrammetric plate LC-HABS-GS07-1116-112L. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  18. 19. INTERIOR: ARCH AND CORBELED VAULT NEAR FRONT Copy photograph ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. INTERIOR: ARCH AND CORBELED VAULT NEAR FRONT Copy photograph of photogrammetric plate LC-HABS-GS07-1116-113L. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  19. 16. INTERIOR: REAR BALCONY LOOKING TOWARD FRONT Copy photograph of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. INTERIOR: REAR BALCONY LOOKING TOWARD FRONT Copy photograph of photogrammetric plate LC-HABS-GS07-1116-110R. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  20. 15. INTERIOR: REAR BALCONY LOOKING TOWARD FRONT Copy photograph of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. INTERIOR: REAR BALCONY LOOKING TOWARD FRONT Copy photograph of photogrammetric plate LC-HABS-GS07-1116-109L. - Provident Life & Trust Company Bank, 407-409 Chestnut Street, Philadelphia, Philadelphia County, PA

  1. 44 CFR 5.26 - Rules for public inspection and copying.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...: 26 Federal Plaza, Suite 1337, New York, New York 10278. Region III: 615 Chestnut Street, One... Center, 130 228th Street SW, Bothell, Washington 98021. (b) Time. Materials will be made available for...

  2. Contextual view to northnorthwest of Burton Park Club House and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Contextual view to north-northwest of Burton Park Club House and Amphitheater (90 mm lens). - Burton Park, Club House & Amphitheater, Adjacent ot south end of Chestnut Avenue, San Carlos, San Mateo County, CA

  3. Antioxidant potential of polyphenols and tannins from burs of Castanea mollissima Blume.

    PubMed

    Zhao, Shan; Liu, Jie Yuan; Chen, Si Yu; Shi, Ling Ling; Liu, Yu Jun; Ma, Chao

    2011-10-12

    Spiny burs of Castanea mollissima Blume (Chinese chestnut) are usually discarded as industrial waste during post-harvesting processing. The objective of this study was to establish an extraction and isolation procedure for tannins from chestnut burs, and to assess their potential antioxidant activity. Aqueous ethanol solution was used as extraction solvent, and HPD 100 macroporous resin column was applied for isolation. The influence of solvent concentration in the extraction and elution process on extraction yield, tannins and polyphenols content, as well as antioxidant potential, including DPPH and ABTS radical scavenging ability, reducing power ability and cellular antioxidant ability were assessed. In both the extraction and isolation process, 50% aqueous ethanol led to superior total tannins and polyphenols content as well as significantly higher antioxidant activity. In addition, the antioxidant activity and the total tannins content in extracts and fractions had a positive linear correlation, and the predominant components responsible for antioxidant activities were characterized as hydrolysable tannins. To the best of our knowledge, this is the first report on the enrichment of tannins from burs of C. mollissim using macroporous resin chromatography, and to assess the cellular antioxidant activity of them.

  4. Multiple introductions of divergent genetic lineages in an invasive fungal pathogen, Cryphonectria parasitica, in France.

    PubMed

    Dutech, C; Fabreguettes, O; Capdevielle, X; Robin, C

    2010-08-01

    The occurrence of multiple introductions may be a crucial factor in the successful establishment of invasive species, but few studies focus on the introduction of fungal pathogens, despite their significant effect on invaded habitats. Although Cryphonectria parasitica, the chestnut blight fungus introduced in North America and Europe from Asia during the 20th century, caused dramatic changes in its new range, the history of its introduction is not well retraced in Europe. Using 10 microsatellite loci, we investigated the genetic diversity of 583 isolates in France, where several introductions have been hypothesized. Our analyses showed that the seven most frequent multilocus genotypes belonged to three genetic lineages, which had a different and geographically limited distribution. These results suggest that different introduction events occurred in France. Genetic recombination was low among these lineages, despite the presence of the two mating types in each chestnut stand analysed. The spatial distribution of lineages suggests that the history of introductions in France associated with the slow expansion of the disease has contributed to the low observed rate of recombination among the divergent lineages. However, we discuss the possibility that environmental conditions or viral interactions could locally reduce recombination among genotypes.

  5. Forest regeneration composition and development in upland, mixed-oak forests.

    PubMed

    Fei, Songlin; Gould, Peter J; Steiner, Kim C; Finley, James C; McDill, Marc E

    2005-12-01

    Advance regeneration in 52 mature mixed-oak stands was analyzed and described. Red maple (Acer rubrum L.) was the most abundant species in the study area. Among oak (Quercus) species, northern red oak (Q. rubra L.) was the most abundant within the Allegheny Plateau physiographic province, whereas chestnut oak (Q. montana L.) was the most abundant within the Ridge and Valley physiographic province. Sixteen stands, for which data are available through the fourth growing season following harvest, were used to describe stand development. Cumulative height, a composite measure of size and density, was used to describe early stand development. Black gum (Nyssa sylvatica Marsh.) and black birch (Betula lenta L.) had dramatic increases in stand density and cumulative height after overstory removal. Cumulative height of northern red oak and chestnut oak showed a faster positive response to overstory removal than red maple. Oak retained its dominance in cumulative height for at least 4 years after harvest. Red maple nevertheless remained the most abundant tree species after overstory removal. Our results suggest that the principal advantage of red maple regeneration is its ability to accumulate in large numbers prior to harvest.

  6. Authentication of the botanical and geographical origin of honey by front-face fluorescence spectroscopy.

    PubMed

    Ruoff, Kaspar; Luginbühl, Werner; Künzli, Raphael; Bogdanov, Stefan; Bosset, Jacques Olivier; von der Ohe, Katharina; von der Ohe, Werner; Amado, Renato

    2006-09-06

    Front-face fluorescence spectroscopy, directly applied on honey samples, was used for the authentication of 11 unifloral and polyfloral honey types (n = 371 samples) previously classified using traditional methods such as chemical, pollen, and sensory analysis. Excitation spectra (220-400 nm) were recorded with the emission measured at 420 nm. In addition, emission spectra were recorded between 290 and 500 nm (excitation at 270 nm) as well as between 330 and 550 nm (excitation at 310 nm). A total of four different spectral data sets were considered for data analysis. Chemometric evaluation of the spectra included principal component analysis and linear discriminant analysis; the error rates of the discriminant models were calculated by using Bayes' theorem. They ranged from <0.1% (polyfloral and chestnut honeys) to 9.9% (fir honeydew honey) by using single spectral data sets and from <0.1% (metcalfa honeydew, polyfloral, and chestnut honeys) to 7.5% (lime honey) by combining two data sets. This study indicates that front-face fluorescence spectroscopy is a promising technique for the authentication of the botanical origin of honey and may also be useful for the determination of the geographical origin within the same unifloral honey type.

  7. Environmental Factors that Influence Physiological Functioning of Eight Bottomland Hardwood Species

    NASA Astrophysics Data System (ADS)

    Kassahun, Z.; Renninger, H. J.

    2017-12-01

    With increases in extreme precipitation, flooding, and prolonged drought events in the southeastern United States, bottomland hardwood forests are expected to experience a drastic shift in their productivity and composition. As environmental conditions shift, certain tree species may experience an increase in productivity or could be more negatively affected over more resilient species, leading to a shift in species composition, water use, and carbon uptake. The goals of this research were to use sap flow measurements, leaf phenology, and photosynthetic rates to study species-specific responses to environmental drivers. Sap flow of eight co-occurring hardwood species as well as soil moisture and vapor pressure deficit were measured continuously over the course of a calendar year that included drought conditions and extended saturated soil conditions. We found that cherrybark oak used the most water during the growing season, about 20% more water than the next highest consumer, swamp chestnut oak. Given low, ample or saturated soil moisture conditions, we found that sap flow of winged elm, American elm, cherrybark oak, and shagbark hickory exhibited varying relationships with vapor pressure deficit under the different soil moisture conditions. While the relationship between sap flow and vapor pressure deficit did not differ depending on soil moisture in willow oak, swamp chestnut oak, and green ash. This suggests that winged elm, American elm, cherrybark oak, and shagbark hickory may be more negatively affected by drought conditions while willow oak, swamp chestnut oak, and green ash are more drought tolerant. Regarding leaf phenology, willow oak, cherrybark oak, and shagbark hickory were the first to experience leaf abscission at the end of the growing season when extended drought conditions occurred. In terms of leaf gas exchange, green ash exhibited the highest photosynthesis and transpiration rates, resulting in the lowest water-use efficiency compared with other study species. Taken together, these responses can be used to estimate forest water budgets given stand species composition or to predict individual species resilience or adaptation to a changing climate, which can improve land surface models and identify species in this forest type that will be most successful under future climate conditions.

  8. 3. Photocopy of circa 1910 photograph showing a general view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopy of circa 1910 photograph showing a general view of the building, published in a 1911 book, Stanley's Views of Philadelphia. - Philadelphia Trust, Safe Deposit & Insurance Company, 415 Chestnut Street, Philadelphia, Philadelphia County, PA

  9. 25. VIEW FOURTH FLOOR, ELEVATOR PULLEY TRANSPORT SYSTEM, LOOKING AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. VIEW FOURTH FLOOR, ELEVATOR PULLEY TRANSPORT SYSTEM, LOOKING AT TOP MAIN WOOD AND STEEL BEAM SUPPORT SYSTEM, NORTHWEST - Bates Manufacturing Company, Storehouse, Northeast corner of Chestnut Street & Hines Alley, Lewiston, Androscoggin County, ME

  10. Influence of aging conditions on the quality of red Sangiovese wine.

    PubMed

    Castellari, M; Piermattei, B; Arfelli, G; Amati, A

    2001-08-01

    A red Sangiovese wine was stored in barrels of different woods (oak and chestnut) and types (225-L "barriques" and 1000-L barrels) at 12 and 22 degrees C for 320 days to evaluate the effects of different aging conditions on wine quality. Chestnut barrels led to wines richer in phenolics, and which were more tannic, colored, and fruity. Oak barrels gave wines with more monomeric phenolics, but less astringent, with higher vanilla smell, and more harmonious. The type of barrel could be used as a parameter to regulate the extraction of wood components and the polymerization of monomeric phenolics. Storage at 22 degrees C favored the formation of polymerized phenolics and the increase of color density and color hue. The temperature produced less pronounced effects on aroma and taste, even if wines stored at 12 degrees C showed more harmony.

  11. Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes.

    PubMed

    Zhang, Dong-Xiu; Nuss, Donald L

    2016-02-23

    Transmission of mycoviruses that attenuate virulence (hypovirulence) of pathogenic fungi is restricted by allorecognition systems operating in their fungal hosts. We report the use of systematic molecular gene disruption and classical genetics for engineering fungal hosts with superior virus transmission capabilities. Four of five diallelic virus-restricting allorecognition [vegetative incompatibility (vic)] loci were disrupted in the chestnut blight fungus Cryphonectria parasitica using an adapted Cre-loxP recombination system that allowed excision and recycling of selectable marker genes (SMGs). SMG-free, quadruple vic mutant strains representing both allelic backgrounds of the remaining vic locus were then produced through mating. In combination, these super donor strains were able to transmit hypoviruses to strains that were heteroallelic at one or all of the virus-restricting vic loci. These results demonstrate the feasibility of modulating allorecognition to engineer pathogenic fungi for more efficient transmission of virulence-attenuating mycoviruses and enhanced biological control potential.

  12. The use of tannin from chestnut (Castanea vesca).

    PubMed

    Krisper, P; Tisler, V; Skubic, V; Rupnik, I; Kobal, S

    1992-01-01

    After mimosa and quebracho extracts, chestnut extract is the third most important vegetable tannin used for leather production. It is produced only in Europe on the northern side of the Mediterranean sea. The extract is prepared by hot water extraction of the bark and timber, followed by spray-drying of the solution. Analysis shows that there are insignificant variations in extract quality between batches, so the extract can be used with modern automated leather production systems. The extract contains approximately 75 percent active tanning substances. The primary component is castalagin, along with smaller amounts of vescalagin, castalin, and vescalin. A castalagin-based pharmaceutical product is currently in use for prevention and treatment of diarrhea in pigs and cattle that is caused by changes in diet. The beneficial effect is due to prevention of water losses through mucous membranes. The castalagin may also form chelates with iron, which influences the reabsorption of the metal in the animal digestive tract.

  13. Spotted phenotypes in horses lost attractiveness in the Middle Ages

    PubMed Central

    Wutke, Saskia; Benecke, Norbert; Sandoval-Castellanos, Edson; Döhle, Hans-Jürgen; Friederich, Susanne; Gonzalez, Javier; Hallsson, Jón Hallsteinn; Hofreiter, Michael; Lõugas, Lembi; Magnell, Ola; Morales-Muniz, Arturo; Orlando, Ludovic; Pálsdóttir, Albína Hulda; Reissmann, Monika; Ruttkay, Matej; Trinks, Alexandra; Ludwig, Arne

    2016-01-01

    Horses have been valued for their diversity of coat colour since prehistoric times; this is especially the case since their domestication in the Caspian steppe in ~3,500 BC. Although we can assume that human preferences were not constant, we have only anecdotal information about how domestic horses were influenced by humans. Our results from genotype analyses show a significant increase in spotted coats in early domestic horses (Copper Age to Iron Age). In contrast, medieval horses carried significantly fewer alleles for these phenotypes, whereas solid phenotypes (i.e., chestnut) became dominant. This shift may have been supported because of (i) pleiotropic disadvantages, (ii) a reduced need to separate domestic horses from their wild counterparts, (iii) a lower religious prestige, or (iv) novel developments in weaponry. These scenarios may have acted alone or in combination. However, the dominance of chestnut is a remarkable feature of the medieval horse population. PMID:27924839

  14. Root development of acorn-origin oak seedlings in shelterwood stands on the Appalachian Plateau of northern Pennsylvania: 4-year results

    Treesearch

    Patrick H. Brose

    2008-01-01

    Post-fire sprouting of oak seedlings in prescribe-burned shelterwood stands hinges on their root collar diameters exceeding 6.4 mm and root size depends on the type of partial cut and the time since harvest. To better understand this shelterwood stage/time/oak root size relationship, a study was begun in northern Pennsylvania in 2001. Acorns of black, chestnut,...

  15. Environmental Fate of Emamectin Benzoate After Tree Micro Injection of Horse Chestnut Trees

    PubMed Central

    Burkhard, Rene; Binz, Heinz; Roux, Christian A; Brunner, Matthias; Ruesch, Othmar; Wyss, Peter

    2015-01-01

    Emamectin benzoate, an insecticide derived from the avermectin family of natural products, has a unique translocation behavior in trees when applied by tree micro injection (TMI), which can result in protection from insect pests (foliar and borers) for several years. Active ingredient imported into leaves was measured at the end of season in the fallen leaves of treated horse chestnut (Aesculus hippocastanum) trees. The dissipation of emamectin benzoate in these leaves seems to be biphasic and depends on the decomposition of the leaf. In compost piles, where decomposition of leaves was fastest, a cumulative emamectin benzoate degradation half-life time of 20 d was measured. In leaves immersed in water, where decomposition was much slower, the degradation half-life time was 94 d, and in leaves left on the ground in contact with soil, where decomposition was slowest, the degradation half-life time was 212 d. The biphasic decline and the correlation with leaf decomposition might be attributed to an extensive sorption of emamectin benzoate residues to leaf macromolecules. This may also explain why earthworms ingesting leaves from injected trees take up very little emamectin benzoate and excrete it with the feces. Furthermore, no emamectin benzoate was found in water containing decomposing leaves from injected trees. It is concluded, that emamectin benzoate present in abscised leaves from horse chestnut trees injected with the insecticide is not available to nontarget organisms present in soil or water bodies. Environ Toxicol Chem 2014;9999:1–6. © 2014 The Authors. Published 2014 SETAC PMID:25363584

  16. Acute Effusive Pericarditis due to Horse Chestnut Consumption

    PubMed Central

    Edem, Efe; Kahyaoğlu, Behlül; Çakar, Mehmet Akif

    2016-01-01

    Patient: Male, 32 Final Diagnosis: Pericardial effusion related to the consumption of herbal product Symptoms: Dyspnea Medication: Horse chestnut (Aesculus hippocastanum L) Clinical Procedure: Pericardial and pleural effusions were drained through a pericardiopleural window Specialty: Cardiology Objective: Unusual clinical course Background: There are many well-known causes of pericardial effusion, such as cancer metastasis, bacterial or viral pericarditis, and uremic pericarditis; however, no reports exist in the literature demonstrating a pericardial effusion that led to cardiac tamponade following consumption of an herbal remedy. Case Report: A 32-year-old male patient was referred to our cardiology outpatient clinic with a complaint of dyspnea. The patient’s medical history was unremarkable; however, he had consumed 3 boxes of horse chestnut (Aesculus hippocastanum L) paste over the previous 1.5 months. His chest x-ray examination revealed an enlarged cardiac shadow and bilateral pleural effusion. On transthoracic echocardiographic examination, his ejection fraction was found to be 55% with circumferentially extended pericardial effusion that reached 3.9 cm at its maximal thickness. No growth had been detected in the pericardial and pleural biopsies or blood samples; there was no evidence of an infectious process in the physical examination. Based on this information, we diagnosed pericarditis resulting from the use of herbal remedies. This is the first report to demonstrate that herbal remedy consumption may cause this type of clinical condition. Conclusions: Besides other well-known causes, pericardial effusion related to the consumption of herbal remedies should always be considered when treating patients with pericardial effusion caused by unclear etiologies. PMID:27141926

  17. Hydrolysable chestnut tannins for reduction of postweaning diarrhea: Efficacy on an experimental ETEC F4 model.

    PubMed

    Girard, Marion; Thanner, Sophie; Pradervand, Nicolas; Hu, Dou; Ollagnier, Catherine; Bee, Giuseppe

    2018-01-01

    An experimental model for postweaning diarrhea with enterotoxigenic Escherichia coli F4 (ETEC F4) was set up in piglets, and the efficacy of 1% chestnut-tannin extract in preventing diarrhea was subsequently assessed. In a first trial (infection model), 32 Swiss Large White piglets (age: 24 days; average BW: 7.8 ± 0.8 kg) were randomly assigned to two experimental groups (infected [INF], noninfected [NINF]). In a subsequent trial, 72 Swiss Large White piglets (age: 26 days; average BW: 7.4 ± 1.5 kg) were blocked by BW and assigned within block to four experimental groups: NINF-CO: not infected and fed a standard control starter diet (CO); INF-CO: infected and fed the CO diet; NINF-TA: not infected and fed the CO diet supplemented with 1% chestnut extract containing 54% of hydrolysable tannins (TA); and INF-TA: infected and fed the TA diet. Both diets (TA and CO) were formulated to be isocaloric and isoproteic and to meet or surpass the nutritional requirements. In both trials, four days after weaning, piglets assigned to the INF group received an oral suspension of ETEC F4. Fecal score, ETEC shedding in feces (only in trial 2), and growth performance traits were measured for the following 14 days post infection. In both trials, more than 50% of the INF piglets developed diarrhea within six days post infection. Tannins reduced (P < 0.05) the average fecal score, the percentage of piglets in diarrhea, and the duration of diarrhea, whereas feed intake and the average daily gain were unaffected.

  18. Russia: Saratov

    Atmospheric Science Data Center

    2013-04-17

    ... to differences in brightness and texture between bare soil and vegetated land. The chestnut-colored soils in this region are brighter ... of vegetation relative to the nadir camera, which sees more soil. In spring, therefore, the scene is brightest in the vertical view and ...

  19. 27. FIRST FLOOR CENTRAL HALL, EAST WALL, DETAIL OF ENTABLATURE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. FIRST FLOOR CENTRAL HALL, EAST WALL, DETAIL OF ENTABLATURE SHOWING EGG AND DART OVOLO AND GUTTAE OF THE THIRD MUTULE FROM THE SOUTHEAST CORNER - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  20. 76 FR 38891 - Companies Holding Certificates of Authority as Acceptable Sureties on Federal Bonds and as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ..., RI, SC, VT, VA, WI. INCORPORATED IN: Michigan. COLONIAL AMERICAN CASUALTY AND SURETY COMPANY (NAIC.... COLONIAL SURETY COMPANY (NAIC 10758) BUSINESS ADDRESS: 50 Chestnut Ridge Road, Montvale, NJ 07645. PHONE...

  1. 76. Photocopy of photograph (original print in the Minnesota Historical ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    76. Photocopy of photograph (original print in the Minnesota Historical Society, St. Paul, Minnesota, 1860. #22358.) UPPER LEVEE FROM CHESTNUT STREET, 1860 - Smith Avenue High Bridge, Smith Avenue between Cherokee Avenue & Cliff Street, Saint Paul, Ramsey County, MN

  2. GOVERNOR'S COUNCIL CHAMBER; SOUTH WALL, UPPER MIDDLE. Glass plate stereopair ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    GOVERNOR'S COUNCIL CHAMBER; SOUTH WALL, UPPER MIDDLE. Glass plate stereopair number PA-1430-139 LC-HABS-GS05-GC-S-4 157.4836. Right (not printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  3. 2. GENERAL VIEW OF INTERIOR. NOTE SKYLIGHTS ON VAULTED CEILING. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. GENERAL VIEW OF INTERIOR. NOTE SKYLIGHTS ON VAULTED CEILING. Photocopy of circa 1910 photo published in a 1911 book, Stanley's Views of Philadelphia. - Pennsylvania Company for Insurances on Lives & Granting Annuities, 431 Chestnut Street, Philadelphia, Philadelphia County, PA

  4. TOWER STAIRHALL; SOUTH SOFFIT, SOUTH WEST (R). Glass plate stereopair ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    TOWER STAIRHALL; SOUTH SOFFIT, SOUTH WEST (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-TS-D-26 (R) 157.4815. Left (printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  5. TOWER STAIRHALL; SOUTH SOFFIT, SOUTH WEST (R). Glass plate stereopair ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    TOWER STAIRHALL; SOUTH SOFFIT, SOUTH WEST (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-TS-D-26 (R) 157.4815. Right (not printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  6. 1. Photocopy of lithograph (from Annual Report of the Supervising ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopy of lithograph (from Annual Report of the Supervising Architect to the Secretary for the Calendar Year Ending December 31, 1888) GENERAL VIEW, SOUTH (FRONT) ELEVATION - Old U.S. Mint, Chestnut & Juniper Streets, Philadelphia, Philadelphia County, PA

  7. The Effects of Intermittent Flooding on Seedlings of Three Forest Species

    Treesearch

    P.H. Anderson; S.R. Pezeshki

    1999-01-01

    Under greenhouse conditions, seedlings of three forest species, baldcypress (Taxodium distichum), nuttall oak (Quercus nuttallii), and swamp chestnut oak (Quercus michauxii) were subjected to an intermittent flooding and subsequent physiological and growth responses to such conditions were evaluated....

  8. Existing Fish and Wildlife Resources Related to the Southern New Jersey Water Resources Study, Burlington, Camden and Gloucester Counties, New Jersey. Phase I Report.

    DTIC Science & Technology

    1978-05-01

    Cherry, black Prunus serotina Ehrh. Chestnut, American Castanea dentata (Marsh.) Borkh. (sprouts) Cucumbertree Magnolia acurninata L. Dogwood, flowering ...Conrad’s Corema conradii Torr. Deerberry Vaccinium stamineum L. Dewberry Rubus villosus Ait. Dewberry, single- flowered Rubus Villosus enslenii Tratt...Dogwood, alternate-leaved Cornus alternifolia L. Dogwood, flowering Cornus florida L. Dogwood, panicled Cornus paniculata L’Her. Dogwood, silky Cornus

  9. Chilling and heat requirements for flowering in temperate fruit trees

    NASA Astrophysics Data System (ADS)

    Guo, Liang; Dai, Junhu; Ranjitkar, Sailesh; Yu, Haiying; Xu, Jianchu; Luedeling, Eike

    2014-08-01

    Climate change has affected the rates of chilling and heat accumulation, which are vital for flowering and production, in temperate fruit trees, but few studies have been conducted in the cold-winter climates of East Asia. To evaluate tree responses to variation in chill and heat accumulation rates, partial least squares regression was used to correlate first flowering dates of chestnut ( Castanea mollissima Blume) and jujube ( Zizyphus jujube Mill.) in Beijing, China, with daily chill and heat accumulation between 1963 and 2008. The Dynamic Model and the Growing Degree Hour Model were used to convert daily records of minimum and maximum temperature into horticulturally meaningful metrics. Regression analyses identified the chilling and forcing periods for chestnut and jujube. The forcing periods started when half the chilling requirements were fulfilled. Over the past 50 years, heat accumulation during tree dormancy increased significantly, while chill accumulation remained relatively stable for both species. Heat accumulation was the main driver of bloom timing, with effects of variation in chill accumulation negligible in Beijing's cold-winter climate. It does not seem likely that reductions in chill will have a major effect on the studied species in Beijing in the near future. Such problems are much more likely for trees grown in locations that are substantially warmer than their native habitats, such as temperate species in the subtropics and tropics.

  10. Chilling and heat requirements for flowering in temperate fruit trees.

    PubMed

    Guo, Liang; Dai, Junhu; Ranjitkar, Sailesh; Yu, Haiying; Xu, Jianchu; Luedeling, Eike

    2014-08-01

    Climate change has affected the rates of chilling and heat accumulation, which are vital for flowering and production, in temperate fruit trees, but few studies have been conducted in the cold-winter climates of East Asia. To evaluate tree responses to variation in chill and heat accumulation rates, partial least squares regression was used to correlate first flowering dates of chestnut (Castanea mollissima Blume) and jujube (Zizyphus jujube Mill.) in Beijing, China, with daily chill and heat accumulation between 1963 and 2008. The Dynamic Model and the Growing Degree Hour Model were used to convert daily records of minimum and maximum temperature into horticulturally meaningful metrics. Regression analyses identified the chilling and forcing periods for chestnut and jujube. The forcing periods started when half the chilling requirements were fulfilled. Over the past 50 years, heat accumulation during tree dormancy increased significantly, while chill accumulation remained relatively stable for both species. Heat accumulation was the main driver of bloom timing, with effects of variation in chill accumulation negligible in Beijing’s cold-winter climate. It does not seem likely that reductions in chill will have a major effect on the studied species in Beijing in the near future. Such problems are much more likely for trees grown in locations that are substantially warmer than their native habitats, such as temperate species in the subtropics and tropics.

  11. Descriptor data of Castanea accessions at the University of Missouri

    USDA-ARS?s Scientific Manuscript database

    Chestnut, Castanea L., trees were propagated and planted in repositories at the Horticulture and Agroforestry Research Center, New Franklin, Missouri in 1996, 2002, 2009 with additional accessions acquired annually. Trees have been pruned, fertilized, irrigated, and pests controlled following Unive...

  12. SECOND FLOOR LOBBY; EAST WALL, UPPER LEFT (R). Glass plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECOND FLOOR LOBBY; EAST WALL, UPPER LEFT (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-2L-E-2 (R) 157.4825. Right (not printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  13. SECOND FLOOR LOBBY; EAST WALL, UPPER LEFT (R). Glass plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECOND FLOOR LOBBY; EAST WALL, UPPER LEFT (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-2L-E-2 (R) 157.4825. Left (printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  14. SECOND FLOOR LOBBY; EAST WALL, LOWER LEFT (R). Glass plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECOND FLOOR LOBBY; EAST WALL, LOWER LEFT (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-2L-E-1 (R) 157.4824. Left (printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  15. SECOND FLOOR LOBBY; EAST WALL, LOWER LEFT (R). Glass plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECOND FLOOR LOBBY; EAST WALL, LOWER LEFT (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-2L-E-1 (R) 157.4824. Right (not printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  16. SECOND FLOOR LOBBY; EAST WALL, LOWER RIGHT (R). Glass plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECOND FLOOR LOBBY; EAST WALL, LOWER RIGHT (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-2L-E-3 (R) 157.4826. Left (printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  17. SECOND FLOOR LOBBY; EAST WALL, LOWER RIGHT (R). Glass plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECOND FLOOR LOBBY; EAST WALL, LOWER RIGHT (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-2L-E-3 (R) 157.4826. Right (not printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  18. SECOND FLOOR LOBBY; EAST WALL, UPPER RIGHT (R). Glass plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECOND FLOOR LOBBY; EAST WALL, UPPER RIGHT (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-2L-E-4 (R) 157.4827. Right (not printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  19. SECOND FLOOR LOBBY; EAST WALL, UPPER RIGHT (R). Glass plate ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECOND FLOOR LOBBY; EAST WALL, UPPER RIGHT (R). Glass plate stereopair number PA-1430-139 LC-HABS-GS05-2L-E-4 (R) 157.4827. Left (printed) - Independence Hall Complex, Independence Hall, 500 Chestnut Street, Philadelphia, Philadelphia County, PA

  20. Foliar nutrient concentrations of oak, hickory, and red maple

    Treesearch

    Amy J. Scherzer; Robert P. Long; Joanne Rebbeck

    2003-01-01

    Early autumn foliar nutrient concentrations of overstory oak (white oak [Quercus alba L.] or chestnut oak [Q. prinus L.]) understory hickory (mockernut hickory [Carya tomentosa (Poir.) Nutt.] or pignut hickory [C. glabra (Mill.) Sweet]), and both overstory and understory red maple (...

  1. 1. Photocopy of circa 1910 photograph showing a general view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopy of circa 1910 photograph showing a general view of the ten-story addition, published in a 1911 book, Stanley's Views of Philadelphia. - Provident Life & Trust Company Bank, 1888-1890 Addition, 401-407 Chestnut Street, Philadelphia, Philadelphia County, PA

  2. East façade, Burton Park Club House, with Amphitheater in foreground, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    East façade, Burton Park Club House, with Amphitheater in foreground, view to north from Amphitheater stage (90 mm lens). - Burton Park, Club House & Amphitheater, Adjacent ot south end of Chestnut Avenue, San Carlos, San Mateo County, CA

  3. Fungi from industrial tannins: potential application in biotransformation and bioremediation of tannery wastewaters.

    PubMed

    Prigione, Valeria; Trocini, Bruno; Spina, Federica; Poli, Anna; Romanisio, Davide; Giovando, Samuele; Varese, Giovanna Cristina

    2018-05-01

    Tannins are a complex family of polyphenolic compounds, widely distributed in the plant kingdom where they act as growth inhibitors towards many microorganisms including bacteria, yeasts, and fungi. Tannins are one of the major components of tannery wastewaters and may cause serious environmental pollution. In the present study, four different tannins (the hydrolysable chestnut ellagitannin and tara gallotannin and the condensed quebracho and wattle tannins) were characterized from a mycological point of view with the aim of selecting fungal strains capable of growing in the presence of high tannin concentration and thus potentially useful in industrial biotransformations of these compounds or in the bioremediation of tannery wastewaters. A total of 125 isolates of filamentous fungi belonging to 10 species and four genera (Aspergillus, Paecilomyces, Penicillium, and Talaromyces) were isolated from the tannin industrial preparations. Miniaturized biotransformation tests were set up with 10 fungal strains and the high-performance liquid chromatography (HPLC) analysis pointed out a strong activity of all the tested fungi on both chestnut and tara tannins. Two strains (Aspergillus tubingensis MUT 990 and Paecilomyces variotii MUT 1125), tested against a real tannery wastewater, were particularly efficient in chemical oxygen demand (COD) and tannin removal (> 60%), with a detoxification above 74%. These results indicate that these fungi are potentially exploitable in the treatment of tannery wastewaters.

  4. Looking for variable molecular markers in the chestnut gall wasp Dryocosmus kuriphilus: first comparison across genes.

    PubMed

    Bonal, Raúl; Vargas-Osuna, Enrique; Mena, Juan Diego; Aparicio, José Miguel; Santoro, María; Martín, Angela

    2018-04-04

    The quick spread of the chestnut gall wasp Dryocosmus kuriphilus in Europe constitutes an outstanding example of recent human-aided biological invasion with dramatic economic losses. We screened for the first time a set of five nuclear and mitochondrial genes from D. kuriphilus collected in the Iberian Peninsula, and compared the sequences with those available from the native and invasive range of the species. We found no genetic variability in Iberia in none of the five genes, moreover, the three genes compared with other European samples showed no variability either. We recorded four cytochrome b haplotypes in Europe; one was genuine mitochondrial DNA and the rest nuclear copies of mitDNA (numts), what stresses the need of careful in silico analyses. The numts formed a separate cluster in the gene tree and at least two of them might be orthologous, what suggests that the invasion might have started with more than one individual. Our results point at a low initial population size in Europe followed by a quick population growth. Future studies assessing the expansion of this pest should include a large number of sampling sites and use powerful nuclear markers (e. g. Single Nucleotide Polymorphisms) to detect genetic variability.

  5. STUDIES IN THE STORAGE OF CHESTNUTS TREATED WITH GAMMA RADIATION. II

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Iwata, T.; Ogata, K.

    1961-03-01

    Two varieties of chestnuts were irradiated with relatively high doses of gamma rays, purporting the extension of storage life of the nuts through the inhibition of rooting and sprouting. The materials were treated with the doses of 1.5, 3, and 6 x 10>s4/sup / 6 weeks after harvest and then stored in moist sawdust at 20 deg C. An almost complete inhibiting effect was obtained with all of the doses used regardless of varieties. The contents of ascorbic acid and of reducing sugar were not influenced directly by any dose of irradiation, but the content of non-reducing sugar was affectedmore » to some extent. A sharp increase of respiration was found both in the whole nuts and in the cotyledon part of the nuts immediately after irradiation. In the embryonic axis part of the irradiated nuts, the respiration did not show any change for a considerable period after irradiation; it became fairly lower than the control from the time when some control nuts initiated rooting, and the discoloration of this part took place about the same time. There was a rapid increase of rot incidence when the irradiated nuts have been stored for 4 months at 20 deg C. (auth)« less

  6. The role of geography and host abundance in the distribution of parasitoids of an alien pest

    PubMed Central

    Nováková, Petra; Holuša, Jaroslav

    2016-01-01

    Chalcid wasps (Hymenoptera: Chalcidoidea) are probably the most effective and abundant parasitoids of the horse chestnut leaf miner (Cameraria ohridella), an alien pest in Europe that lacks specialized enemies. We studied how the species richness and abundance of chalcids are influenced by altitude, direction of an alien spread and host abundance of C. ohridella. We quantified the numbers and species richness of chalcid wasps and the numbers of C. ohridella that emerged from horse chestnut (Aesculus hippocastanum) leaf litter samples collected from 35 sites in the Czech Republic. Species richness of chalcids, which was considered an indicator of the possible adaptation of parasitoids to this alien host, was unrelated to C. ohridella abundance, direction of spread, or altitude. Chalcid abundance, which was considered an indicator of parasitism of the alien host, was strongly and positively related to C. ohridella abundance. Chalcid abundance was negatively related to direction of spread and positively related, although in a non-linear manner, to altitude. The relationship of chalcid abundance with direction of spread and altitude was weaker than that with C. ohridella abundance. The results provide evidence that biological control of the alien pest C. ohridella by natural enemies might develop in the future. PMID:26819849

  7. Host tracking or cryptic adaptation? Phylogeography of Pediobius saulius (Hymenoptera, Eulophidae), a parasitoid of the highly invasive horse-chestnut leafminer

    PubMed Central

    Hernández-López, Antonio; Rougerie, Rodolphe; Augustin, Sylvie; Lees, David C; Tomov, Rumen; Kenis, Marc; Çota, Ejup; Kullaj, Endrit; Hansson, Christer; Grabenweger, Giselher; Roques, Alain; López-Vaamonde, Carlos

    2012-01-01

    Classical biological control is often advocated as a tool for managing invasive species. However, accurate evaluations of parasitoid species complexes and assessment of host specificity are impeded by the lack of morphological variation. Here, we study the possibility of host races/species within the eulophid wasp Pediobius saulius, a pupal generalist parasitoid that parasitize the highly invasive horse-chestnut leaf-mining moth Cameraria ohridella. We analysed the population genetic structure, host associations and phylogeographic patterns of P. saulius in Europe using the COI mitochondrial gene. This marker strongly supports a division into at least five highly differentiated parasitoid complexes, within two of which clades with differing degrees of host specialization were found: a Balkan clade that mainly (but not only) attacks C. ohridella and a more generalist European group that attacks many hosts, including C. ohridella. The divergence in COI (up to 7.6%) suggests the existence of cryptic species, although this is neither confirmed by nuclear divergence nor morphology. We do not find evidence of host tracking. The higher parasitism rates observed in the Balkans and the scarcity of the Balkan–Cameraria haplotypes out of the Balkans open the possibility of using these Balkan haplotypes as biological control agents of C. ohridella elsewhere in Europe. PMID:25568046

  8. Selective Behaviour of Honeybees in Acquiring European Propolis Plant Precursors.

    PubMed

    Isidorov, Valery A; Bakier, Sławomir; Pirożnikow, Ewa; Zambrzycka, Monika; Swiecicka, Izabela

    2016-06-01

    Honey bees harvest resins from various plant species and use them in the hive as propolis. While there have been a number of studies concerning the chemical composition of this antimicrobial product, little is known about selective behavior and bee preference when different potential plant sources of resin are available. The main objective of this paper was to investigate some aspects of behavioral patterns of honeybees in the context of resin acquisition. Samples of propolis originating from temperate zones of Europe and the supposed botanical precursors of the product were analyzed. Taxonomical markers of bud resins of two white birch species, aspen, black poplar, horse-chestnut, black alder, and Scots pine were determined through GC-MS analysis. All these trees have been reported as sources of propolis, but comparisons of the chemical composition of their bud resins with the compositions of propolis samples from seven European countries have demonstrated the presence of taxonomical markers only from black poplar, aspen, and one species of birch. This suggests selective behavior during the collection of bud resins by honeybees. To examine the causes of such selectivity, the antimicrobial properties of bud resins were determined. Horse-chestnut resins had lower antimicrobial activity than the other resins which did not differ significantly.

  9. A tangled tale of two teal: Population history of the grey Anas gracilis and chestnut teal a. castanea of Australia

    USGS Publications Warehouse

    Joseph, L.; Adcock, G.J.; Linde, C.; Omland, K.E.; Heinsohn, R.; Terry, Chesser R.; Roshier, D.

    2009-01-01

    Two Australian species of teal (Anseriformes: Anatidae: Anas), the grey teal Anas gracilis and the chestnut teal A. castanea, are remarkable for the zero or near-zero divergence recorded between them in earlier surveys of mitochondrial DNA (mtDNA) diversity. We confirmed this result through wider geographical and population sampling as well as nucleotide sampling in the more rapidly evolving mtDNA control region. Any data set where two species share polymorphism as is the case here can be explained by a model of gene flow through hybridization on one hand or by incomplete lineage sorting on the other hand. Ideally, analysis of such shared polymorphism would simultaneously estimate the likelihood of both phenomena. To do this, we used the underlying principle of the IMa package to explore ramifications to understanding population histories of A. gracilis and A. castanea. We cannot reject that hybridization occurs between the two species but an equally or more plausible finding for their nearly zero divergence is incomplete sorting following very recent divergence between the two, probably in the mid-late Pleistocene. Our data add to studies that explore intermediate stages in the evolution of reciprocal monophyly and paraphyletic or polyphyletic relationships in mtDNA diversity among widespread Australian birds. ?? 2009 J. Avian Biol.

  10. Optimization of water curing for the preservation of chestnuts (Castanea sativa Mill.) and evaluation of microbial dynamics during process.

    PubMed

    Blaiotta, Giuseppe; Di Capua, Marika; Romano, Annalisa; Coppola, Raffaele; Aponte, Maria

    2014-09-01

    Chestnuts are very perishable fruits, whose quality may be compromised during postharvest handling. Damage can be caused both by insects and fungi. Water curing, a commonly used postharvest method, is based on soaking fruits in water typically for about one week. Factors that affect effectiveness of water curing have only been explained partially. A decrease in pH, likely imputable to a light fermentation caused by lactic acid bacteria, may inhibit the growth of moulds. In this study a Lactobacillus pentosus strain was selected for its ability to inhibit fungi, and used as a starter culture during water curing. As second goal, a reduction of the environmental impact of the process was evaluated by using water that had been re-cycled from a previous curing treatment. Experiments were performed on pilot as well as on farm scale. In all trials, microbial dynamics were evaluated by means of a polyphasic approach including conventional and molecular-based analyses. According to results, the employment of an adjunct culture appears as a very promising opportunity. Even if no reduction in the duration of the process was achieved, waters exhibited a minor microbial complexity and fruits did not lose the natural lustre after the process. Copyright © 2014 Elsevier Ltd. All rights reserved.

  11. Evaluation of organic carbon stocks and CO2 fluxes in grasslands of Western Transbaikalia

    NASA Astrophysics Data System (ADS)

    Lavrentyeva, I. N.; Merkusheva, M. G.; Ubugunov, L. L.

    2017-04-01

    The stocks of organic carbon and mean rates of the CO2 emission during the growing season (May-September) and the entire year were estimated in a sequence of grass ecosystems along the transect encompassing chestnut and meadow-chestnut steppe soils, marsh and meadow alluvial soils, and a haloxerophytic community on a typical solonchak. The total stocks of organic carbon comprised 6.17-9.70 kg C/m2 in steppe, 7.41-10.04 kg C/m2 in floodplain, and 4.74 kg C/m2 in haloxerophytic ecosystems. The portion of humus carbon in the upper 50-cm-thick soil layer comprised 79-92% of the total carbon stock. The mean daily CO2 emission (C-CO2/(m2 day)) from alluvial soils was moderate (3.3-4.9) or low (1.5-2.5). The dependence of the CO2 emission on the moistening of steppe soils, temperature of alluvial soils, and temperature and moistening of solonchak was revealed. In comparison with the CO2 emission from the zonal chestnut soil, its mean values during the growing season and the entire year were 1.2 times higher for the meadowchestnut soil, 3.3 times higher for the marsh alluvial soil, 2.3 times higher for the meadow alluvial soil, and 1.7 times higher for the solonchak. The portion of the CO2 emission beyond the growing season in the mean annual emission averaged 19.8-24.2% and depended on the type of grass ecosystem and on weather conditions of particular years. The sink of carbon in the grass ecosystems exceeded carbon emission, especially in the steppe ecosystems.

  12. 1. GENERAL VIEW, FROM SOUTHEAST. TWOSTORY AND DEMOLISHED SECTIONS ARE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. GENERAL VIEW, FROM SOUTHEAST. TWO-STORY AND DEMOLISHED SECTIONS ARE THE NEW HALL; THE BUILDING TO THE RIGHT IS THE FRONT STORE (HABS No. PA-1398-A) - Carpenters' Company, New Hall, Carpenters' Court & 322 Chestnut Street (rear), Philadelphia, Philadelphia County, PA

  13. A Kaleidoscope of Cultures and the Arts.

    ERIC Educational Resources Information Center

    Drago, Marganne

    This workshop presentation offers an introduction to and suggestions for a multicultural approach to teaching through music experiences. Experiences presented include: (1) "Funga Alafia," a welcome song from Liberia; (2) "Under the Spreading Chestnut Tree," an American traditional game song; (3) a Japanese version of…

  14. 76 FR 77991 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-15

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice...-000. Applicants: Sandy Ridge Wind, LLC. Description: Request for Category 1 Seller Status to be.... Docket Numbers: ER12-571-000. Applicants: Chestnut Flats Wind, LLC. Description: Request for Category 1...

  15. Physiological and structural foliar characteristics of four central Pennsylvania barrens species in contrasting light regimes

    Treesearch

    B.D. Kloeppel; M.E. Kubiske; M.D. Abrams

    1991-01-01

    Four central Pennsylvania barrens species, black oak (Quercus velutina), chestnut oak (Quercus prinus), red maple (Acer rubrum), and sassafras (Sassafras albidum), in the sapling size range were tagged and monitored in juxtaposed understory and full sunlight conditions.

  16. Gypsy moth IPM

    Treesearch

    Michael L. McManus; Andrew M. Liebhold

    2009-01-01

    Over the last 50 years, North American forests have been inundated by a multitude of alien pest invasions. Among these, noteworthy invaders include the hemlock woolly adelgid (Adelges tsugae), emerald ash borer (Agrilus planipennis), chestnut blight and Dutch elm disease. These species have greatly altered both the ecological and...

  17. Bactericidal effect of hydrolysable and condensed tannin extracts on Campylobacter jejuni in vitro

    USDA-ARS?s Scientific Manuscript database

    Strategies are sought to reduce intestinal colonization of food-producing animals by Campylobacter jejuni, a leading bacterial cause of human foodborne illness worldwide. Presently, we tested the antimicrobial activity of hydrolysable-rich blackberry, cranberry, chestnut tannin extracts, and conden...

  18. Elevation Request Letter to Army - signed December 22, 2000

    EPA Pesticide Factsheets

    A letter to request a review of a decision to issue four Rivers and Harbors Act Section 10 permits to deepen channels in Chestnut Cove, Frog Mortar Creek, and Greyhound Cove in Baltimore County, and in Grays Creek in Anne Arundel County, MD.

  19. Leaf gas exchange of mature bottomland oak trees

    Treesearch

    Rico M. Gazal; Mark E. Kubiske; Kristina F. Connor

    2009-01-01

    We determined how changes in environmental moisture affected leaf gas exchange in Nuttall (Quercus texana Buckley), overcup (Q. lyrata Walt.), and dominant and codominant swamp chestnut (Q. michauxii Nutt.) oak trees in Mississippi and Louisiana. We used canopy access towers to measure leaf level gas...

  20. 11. VIEW OF WASHINGTON SQUARE LOOKING WEST (top) BETWEEN SEVENTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. VIEW OF WASHINGTON SQUARE LOOKING WEST (top) BETWEEN SEVENTH (upper) AND SIXTH (lower) STS. SHOWING PENN MUTUAL LIFE INSURANCE BUILDING (right) AND CURTIS PUBLISHING COMPANY BUILDING (far right) - Independence National Historical Park, Walnut, Sixth, Chestnut & Second Streets, Philadelphia, Philadelphia County, PA

  1. Contextual view to northnorthwest of Burton Park Club House and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Contextual view to north-northwest of Burton Park Club House and Amphitheater (90 mm lens). View taken from top of steps visible in previous photo. - Burton Park, Club House & Amphitheater, Adjacent ot south end of Chestnut Avenue, San Carlos, San Mateo County, CA

  2. Silvical characteristics of the five upland oaks

    Treesearch

    Earl L. Core

    1971-01-01

    The five most important upland oaks of eastern North America are white oak (Quercus alba), chestnut oak (Q. prinus), northern red oak (Q. rubra), black oak (Q. velutina), and scarlet oak (Q. coccinea). Of these, white oak and northern red oak are most characteristic of...

  3. Contribution of botanical origin and sugar composition of honeys on the crystallization phenomenon.

    PubMed

    Escuredo, Olga; Dobre, Irina; Fernández-González, María; Seijo, M Carmen

    2014-04-15

    The present work provides information regarding the statistical relationships among the palynological characteristics, sugars (fructose, glucose, sucrose, melezitose and maltose), moisture content and sugar ratios (F+G, F/G and G/W) of 136 different honey types (including bramble, chestnut, eucalyptus, heather, acacia, lime, rape, sunflower and honeydew). Results of the statistical analyses (multiple comparison Bonferroni test, Spearman rank correlations and principal components) revealed the valuable significance of the botanical origin on the sugar ratios (F+G, F/G and G/W). Brassica napus and Helianthus annuus pollen were the variables situated near F+G and G/W ratio, while Castanea sativa, Rubus and Eucalyptus pollen were located further away, as shown in the principal component analysis. The F/G ratio of sunflower, rape and lime honeys were lower than those found for the chestnut, eucalyptus, heather, acacia and honeydew honeys (>1.4). A lower value F/G ratio and lower water content were related with a faster crystallization in the honey. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. Antioxidant activities and structural characterization of flavonol O-glycosides from seeds of Japanese horse chestnut (Aesculus turbinata BLUME).

    PubMed

    Kimura, Hideto; Ogawa, Satoshi; Ishihara, Tomoe; Maruoka, Mahoko; Tokuyama-Nakai, Shota; Jisaka, Mitsuo; Yokota, Kazushige

    2017-08-01

    We attempted to evaluate the contents and distribution of antioxidants in the whole seeds, seed shells, and peeled seeds of the Japanese horse chestnut. The seed shells exhibited the highest antioxidant activities due to the presence of highly polymeric proanthocyanidins as we have reported recently. On the other hand, the peeled seeds predominantly contained flavonols such as quercetin and kaempferol at a high level of 66.7% of total polyphenols, also contributing to the predominant antioxidant activities. The instrumental analysis of the extract from the whole seeds revealed the identification of eight flavonol O-glycosides, including six compounds with quercetin and two species with kaempferol as aglycones. The isolated species exhibited different antioxidant activities depending on the types of aglycones, glycosides, and acylated moieties. The results indicate that the peeled seeds are a good source of flavonol O-glycosides serving as antioxidants to be used for food additives and dietary supplements. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Fractionation and structural characterization of polyphenolic antioxidants from seed shells of Japanese horse chestnut (Aesculus turbinata BLUME).

    PubMed

    Ogawa, Satoshi; Kimura, Hideto; Niimi, Ai; Katsube, Takuya; Jisaka, Mitsuo; Yokota, Kazushige

    2008-12-24

    Seed shells of the Japanese horse chestnut (Aesculus turbinata BLUME) contain high levels of polyphenolic antioxidants. These compounds were extracted, fractionated, and finally separated into three fractions, F1, F2, and F3, according to their degrees of polymerization. The structures of the isolated fractions were characterized by a combination of mass spectrometric analyses. F1 contained mainly low molecular weight phenolic substances, including procyanidin trimers. The predominant fractions F2 and F3 consisted of polymeric proanthocyanidins having a series of heteropolyflavan-3-ols, (+)-catechin/(-)-epicatechin units, and polymerization degrees of 19 and 23, respectively. The polyphenol polymers had doubly linked A-type interflavan linkages in addition to single B-type bonds without gallic acid esterified to them. The isolated polyphenolic compounds exhibited potent antioxidative activities comparable to monomeric (+)-catechin and (-)-epicatechin, or more efficacious than those monomers. The results suggest the potential usefulness of polyphenol polymers from seed shells as a source for nutraceutical factors.

  6. Effect of artificial ageing using different wood chips on the antioxidant activity, resveratrol and catechin concentration, sensory properties and colour of two Greek red wines.

    PubMed

    Gortzi, Olga; Metaxa, Xenia; Mantanis, George; Lalas, Stavros

    2013-12-01

    Two Greek red wines (Syrah and Cabernet) were artificially aged with different wood chips (white oak, red oak, Turkey oak, chestnut, Bosnian pine, cherry, common juniper, common walnut, white mulberry, black locust and apricot). The influence of each wood species was tested for up to 20 days. The optimum duration for the extraction of total polyphenols was 20 days (Syrah) or 10 days (Cabernet) when chips of white oak, chestnut, cherry, white mulberry, black locust and apricot where used. Resveratrol and catechin concentrations ranged within the limits previously reported in literature. A high antioxidant activity was established after 10 days of artificial ageing. The sensory evaluation showed that the best results were produced by the apricot chips after 5 days (Syrah) or black locust and apricot after 5 days (Cabernet). Colour was seen to increase with both time of ageing and number of wood chips added. Copyright © 2013 Elsevier Ltd. All rights reserved.

  7. Efficient photodegradation of organic dye using anatase TiO2 plants as catalyst

    NASA Astrophysics Data System (ADS)

    Bahadur, Jitendra; Pal, Kaushik

    2017-11-01

    Anatase TiO2 hierarchical nanostructures with higher photocatalytic activity are of special importance in various applications. We have reported the synthesis of TiO2 as water chestnut plants like morphology via facile hydrothermal method, by using Titanium (IV) butoxide (TBOT) as a precursor solution. It is found that TiO2 nanoparticles work as seed and completely convert into water chestnut plants like structure or morphology, which are composed of crystallized anatase nanocrystals. X-ray diffraction spectra confirmed the presence of anatase phase of crystallized TiO2 plants (TPs). The average life time delay for generated charge carriers in TPs was calculated to be around 2.45 ns, which reflects slow recombination of charge carriers. The prepared TPs show excellent photocatalytic performance when applied in photo degradation of Rhodamine B organic dye. The unique features exhibited by TPs make them a promising candidate for vast potential applications in field such as solar cells, photocatalysis, supercapacitor, lithium ion batteries and some related fields.[Figure not available: see fulltext.

  8. Chestnut and lemon balm based ingredients as natural preserving agents of the nutritional profile in matured "Serra da Estrela" cheese.

    PubMed

    Carocho, Márcio; Barreira, João C M; Bento, Albino; Fernández-Ruiz, Virginia; Morales, Patricia; Ferreira, Isabel C F R

    2016-08-01

    Chestnut flowers, lemon balm plants and their decoctions were incorporated into "Serra da Estrela" cheese, to assess their potential to preserve its nutritional properties and provide new foodstuffs. The analyses were carried out after the normal ripening period of 1month and after 6months of storage. The most abundant nutrients were proteins and fats. The most abundant minerals were Ca and Na, while C16:0 and C18:1 were the main fatty acids. Saturated fatty acids were the most abundant, followed by the monounsaturated. Moisture seemed to be lower in the samples with the plants incorporated. The dried plants, when incorporated, seemed to be more efficient as preservers then the decoctions, although these better preserved the proteins. These plants can be regarded as promising natural preservers in foodstuffs cheese, given the preservation of key parameters and the slight impact on the nutritional value. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. II. Pathogens

    Treesearch

    Ned B. Klopfenstein; Brian W. Geils

    2011-01-01

    Invasive fungal pathogens have caused immeasurably large ecological and economic damage to forests. It is well known that invasive fungal pathogens can cause devastating forest diseases (e.g., white pine blister rust, chestnut blight, Dutch elm disease, dogwood anthracnose, butternut canker, Scleroderris canker of pines, sudden oak death, pine pitch canker) (Maloy 1997...

  10. 75 FR 67304 - Proposed Flood Elevation Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-02

    ....40 mile None +786 Borough of Twilight. downstream of Fox Stop Road. Approximately 1,800 None +789... inspection at the Borough Building, 1 Procasky Road, Marianna, PA 15345. Borough of Twilight Maps are available are available for inspection at the Twilight Borough Building, 8 Chestnut Road, Charleroi, PA...

  11. Site index curves for black, white, scarlet, and chestnut oaks in the Central States.

    Treesearch

    Willard H. Carmean

    1971-01-01

    Stem analyses showed polymorphic patterns of height growth for each species and for different levels of site quality. New site index curves are presented that show better height growth in later years than predicted by older harmonized site index curves.

  12. Oak Regeneration - What We Know

    Treesearch

    Harvey E. Kennedy

    1989-01-01

    Growth and development of saplings of four-oak (Quercus spp.) species planted at five spacings in a minor stream bottom in southeast Arkansas showed significant differences among species and spacings. Spacing affected all tree size and biomass variables except survival. Yater oak (Q. nigra L.) developed most rapidly; swamp chestnut...

  13. Framing Your Mission

    ERIC Educational Resources Information Center

    Jarrell, Andrea

    2009-01-01

    St. Paul's School in New Hampshire, the Orchard School in Indiana, Chestnut Hill Academy in Pennsylvania, and Dana Hall School in Massachusetts are like most independent schools--they have qualities that are distinctive and extraordinary. Line up their mission statements, however, and the schools sound almost interchangeable. They're all on a…

  14. Inoculum Density Relationships for Infection of Some Eastern US Forest Species by Phytophthora ramorum

    Treesearch

    Paul Tooley; Marsha Browning; Robert Leighty

    2013-01-01

    Our objectives were to establish inoculum density relationships between P. ramorum and selected hosts using detached leaf and whole-plant inoculations. Young plants and detached leaves of Quercus prinus (Chestnut oak), Q. rubra (Northern red oak), Acer rubrum (red maple), ...

  15. Further Applications of Linguistics to Australian Aboriginal Contexts. Occasional Papers Number 8.

    ERIC Educational Resources Information Center

    McKay, G. R., Ed.; Sommer, B. A., Ed.

    These papers represent applications of linguistics in Australian Aboriginal contexts, not only from a language viewpoint, but encompassing the interactions of language, society, politics, and culture. They include: "Loanwords: Ours or Theirs?" (Velma J. Leeding); "We Spell It 'Gooniyandi'" (David Street and Topsy Chestnut);…

  16. 78 FR 19016 - Importer of Controlled Substances; Notice of Registration; Mylan Pharmaceuticals, Inc.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... Registration; Mylan Pharmaceuticals, Inc. By Notice dated November 27, 2012, and published in the Federal Register on December 5, 2012, 77 FR 72409, Mylan Pharmaceuticals, Inc., 781 Chestnut Ridge Road, Morgantown... that the registration of Mylan Pharmaceuticals, Inc. to import the basic classes of controlled...

  17. 77 FR 30326 - Importer of Controlled Substances; Notice Of Registration; Mylan Pharmaceuticals, Inc.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-22

    ... Registration; Mylan Pharmaceuticals, Inc. By Notice dated February 23, 2012, and published in the Federal Register on March 1, 2012, 77 FR 12620, Mylan Pharmaceuticals, Inc., 781 Chestnut Ridge Road, Morgantown...), and determined that the registration of Mylan Pharmaceuticals, Inc. to import the basic classes of...

  18. 77 FR 12620 - Importer of Controlled Substances, Notice of Application, Mylan Pharmaceuticals, Inc.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-01

    ... DEPARTMENT OF JUSTICE Drug Enforcement Administration Importer of Controlled Substances, Notice of Application, Mylan Pharmaceuticals, Inc. Pursuant to 21 U.S.C. 958(i), the Attorney General shall, prior to... on December 30, 2011, Mylan Pharmaceuticals, Inc., 781 Chestnut Ridge Road, Morgantown, West Virginia...

  19. The Explosive Pulsed Power Test Facility at AFRL

    DTIC Science & Technology

    2005-06-01

    Air Force Research Laboratory , AFRL /DEHP, Albuquerque...NM 87117 S. Coffey, A. Brown, B. Guffey NumerEx, Albuquerque, NM Abstract The Air Force Research Laboratory has developed and tested a...Chestnut Site on Kirtland Air Force Base. The facility is described in this paper, including details of recent upgrades. I.

  20. 7 CFR 210.10 - Meal requirements for lunches and requirements for afterschool snacks.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... group served in the school. In addition, school lunches must meet the dietary specifications in... specifications for calories, saturated fat, trans fat, and sodium. Foods of minimal nutritional value and fluid..., green bananas, green peas, green lima beans, plantains, taro, water chestnuts, and white potatoes; and...

  1. Nondestructive inspection of nuts for food quality and safety using NIRS (abstract)

    USDA-ARS?s Scientific Manuscript database

    Mold infection and insect infestation are significant postharvest problems for processors of nuts. Fungal disease causes direct loss of product or reduced value due to the lower-quality grade of the chest-nut lot. In most cases, fungal infection is not detectable using traditional sorting techniques...

  2. 18 CFR 35.32 - General provisions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Chestnut Street, Philadelphia, PA 19104, and are also available in local law libraries. Copies may be inspected at the Federal Energy Regulatory Commission's Library, Room 95-01, 888 First Street, NE... relating to the Fund shall be open to inspection and audit at reasonable times by the utility or its...

  3. Restoring table mountain pine (Pinus pungens Lamb.) communities with prescribed fire: an overview of current research

    Treesearch

    Nicole Turrill Welch; Thomas A. Waldrop

    2001-01-01

    Table mountain pine (Pinus pungens Lamb.) communities of the Southern Appalachian Mountains have been maintained historically by lightning- and human-caused fires. Characteristic stands have a table mountain pine overstory, a chestnut oak (Quercus prinus L.), scarlet oak (Q. coccinea Muenchh.), and blackgum (

  4. 77 FR 58321 - National Oil and Hazardous Substances Pollution Contingency Plan; National Priorities List...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-20

    ... through http://www.regulations.gov or in hard copy at the site information repositories. Locations, contacts, phone numbers and viewing hours are: Regional Site Information Repository: U.S. EPA Record Center... Repository: New Hanover County Public Library 28401, 201 Chestnut Street, Wilmington, North Carolina 28401...

  5. Staff Directory, Department of Vertebrate Zoology, NMNH

    Science.gov Websites

    Research & Collections About Us Get Involved Calendar Department ofVertebrate Zoology Chestnut Mammals VZ Online Newsletter Visitor Information Research Fellowships Volunteers and Interns VZ Libraries Staff Contact Us NMNH Home › Research & Collections › Vertebrate Zoology › Staff Directory

  6. Development of seven hardwood species in small forest openings: 22-year results

    Treesearch

    R.L. Johnson; R.M. Krinard

    1983-01-01

    Small forest openings planted with seven leading commercial tree species were completely dominated by yellow-poplar (Liriodendron tulipifera) that ave raged 90 feet tall after 22 years. Of the other planted species, swamp chestnut oak (Quercus michauxii) was most shade tolerant while shumard oaks (Q. shumardii),...

  7. 75 FR 61772 - Meeting of the Paterson Great Falls National Historical Park Advisory Commission

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ... DEPARTMENT OF THE INTERIOR National Park Service Meeting of the Paterson Great Falls National... 1,10), notice is hereby given of the meeting of the Paterson Great Falls National Historical Park... Great Falls National Historical Park National Park Service, 200 Chestnut Street, Philadelphia, PA 19106...

  8. Trunk injury and fungal transport by Agrilus bilineatus, Chrysobothris femorata, and Xyloterinus sp. in oak wilt infected trees in Texas

    Treesearch

    R. Lewis

    1987-01-01

    Examinations for trunk borers revealed that over 95% of the oak wilt (Ceratocystis fagacearum) infected trees in Texas were infested with twolined chestnut borers (Agrilus bilineatus) and flatheaded appletree borers (Chrysobothris femorata). Infestations by the ambrosia beetle (Xyloterinus sp...

  9. A discriminant analysis of introgression between Quercus prinus L. and Quercus alba L.

    Treesearch

    F. Thomas Ledig; Robert W. Wilson; John W. Duffield; Gerald Maxwell

    1969-01-01

    The natural hybrid between rock chestnut oak, Q. prinus, and white oak, Q. alba, was named Q. x saulii by Schneider (1904). The nomenclature used herein follows that of Little (1953). Q. x saulii is frequently identified throughout the area of sympatry of...

  10. Realistic restoration targets

    Treesearch

    Melissa Thomas-Van Gundy; Robert Whetsell

    2016-01-01

    These reference ecosystems and historic range of variability may be hard to define or determine, but accepted methods can include cultural evidence such as written descriptions, oral histories, maps and photographs, and survey records (Egan and Howell 2001). Another tactic is to focus on restoring species composition (such as returning the American chestnut) and...

  11. 75 FR 32502 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ..., Harrisburg, 10000400 Lebanon County Colebrook Iron Master's House, 5200 Elizabethtown Rd, South Londonderry, 10000405 Salem Evangelical Lutheran Church, 119 N Eighth St, Lebanon, 10000402 Lehigh County Martin Tower..., 8832 Abilene Rd, Farmville, 10000384 Russell County Blackford Bridge, Chestnut Rd (Rte 652), Lebanon...

  12. Colour measurements of surfaces to evaluate the restoration materials

    NASA Astrophysics Data System (ADS)

    Lo Monaco, Angela; Marabelli, Maurizio; Pelosi, Claudia; Picchio, Rodolfo

    2011-06-01

    In this paper two case studies on the application of colour measurements for the evaluation of some restoration materials are discussed. The materials related to the research are: watercolours employed in restoration of wall paintings and preservative/consolidants for wood artifacts. Commercial watercolours, supplied by Maimeri, Windsor&Newton and Talens factories have been tested. Colour measurements have been performed by means of a reflectance spectrophotometer (RS) before and after accelerated ageing of watercolours at 92% relative humidity (RH) and in a Solar Box chamber. The experimental results show that watercolours based on natural earths and artificial ultramarine undergo the main colour changes, expressed as L*, a* and b* variations and total colour difference (▵E*). In the other cases colour differences depend on both watercolour typology and suppliers. The other example concerns the evaluation of colour change due to surface treatment of Poplar (Populus sp.) and chestnut (Castanea sativa L.) wood samples. The wooden samples have been treated with a novel organic preservative/consolidant product that has been tested also in a real case as comparison. The treated samples have been artificially aged in Solar Box chamber equipped with a 280 nm UV filter. Colour has been measured before and after the artificial ageing by means of a RS. Colour changes have been determined also for the main door of an historical mansion in Viterbo, made of chestnut wood, and exposed outdoors.

  13. Evidence for sexual selection on structural plumage coloration in female eastern bluebirds (Sialia sialis).

    PubMed

    Siefferman, Lynn; Hill, Geoffrey E

    2005-08-01

    Although the function of ornamental traits in males has been the focus of intensive research for decades, expression of such traits in females has received much less study. Eastern bluebirds (Sialia sialis) display structurally based ultraviolet/blue and melanin-based chestnut plumage, and in males this plumage coloration is related to both reproductive success and competitive ability. Compared to males, female bluebirds show a subdued expression of blue and chestnut ornamental coloration, and we used a combination of an aviary nutritional-stress experiment and four years of field data to test the hypothesis that coloration functions as a signal of female quality. First, we tested the effect of food intake on expression of structural and melanin coloration in female eastern bluebirds to determine whether structural or melanin coloration are condition-dependent traits. Females that were given ad libitum access to food displayed more ornamented structural coloration than females on a food-restricted diet, but there was no effect of the experiment on melanin ornamentation. Second, we used field data to assess whether female ornamentation correlated with measures of mate quality and parental effort. The structural coloration of females predicted first egg date, maternal provisioning rates, and measures of reproductive success. These data indicate that structural coloration is dependent on nutritional condition and suggest that sexual selection is acting on structurally based plumage coloration in female eastern bluebirds.

  14. Valorization of solid wastes from chestnut industry processing: Extraction and optimization of polyphenols, tannins and ellagitannins and its potential for adhesives, cosmetic and pharmaceutical industry.

    PubMed

    Aires, Alfredo; Carvalho, Rosa; Saavedra, Maria José

    2016-02-01

    The aim of the current study was to evaluate the potential of chestnut peels to produce pomaces enhanced with tannins to be used in the formulations of wood adhesives, leather tanning or as natural antioxidants in food, cosmetic and pharmaceutical industry. An analytical procedure was planned as 2 factorial design to analyze the influence of solvent (water, Na2SO3 and NaOH at different concentrations of 1, 2, 4, and 8% in water) and extraction time (30, 60, and 120, 240, 480 and 960min) on extraction yield, pH, Stiasny index, and tannins. HPLC-diode array detector equipped with an ionization mass spectrophotometer was used to assess the polyphenol composition. Our results showed that both extraction properties and phytochemicals were significantly affected (P<0.001) by all independent factors. The main tannins identified were the hydrolyzable gallic acid, vescalagin castalagin and ellagic acid, and the condensed epigallocatechin, catechin and epicatechin. The solvent 1% Na2SO3 was more effective to extract the condensed tannins whilst hydrolyzable tannins were extracted efficiently by 1% NaOH. The multivariable analysis and the Pearson's correlation coefficients showed a direct association between Stiasny number and the average levels of condensed tannins. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Antitumor, Antioxidant, and Nitrite Scavenging Effects of Chinese Water Chestnut (Eleocharis dulcis) Peel Flavonoids.

    PubMed

    Zhan, Ge; Pan, Leiqing; Tu, Kang; Jiao, Shunshan

    2016-10-01

    The preparation, quantification, and characterization of flavonoid compounds from Chinese water chestnut peel (CWCP) flavonoid extract and ethyl acetate fraction (EF), n-butanol fraction, and water fraction were studied. Among these, EF showed the maximum free radical levels (IC 50 values of 0.36, 0.40, and 0.37 mg/mL for DPPH•, ABTS• + , and •OH, respectively), nitrite scavenging effects (IC 50 = 1.89 mg/mL), and A549 cell inhibitory activities (IC 50 = 776.12 μg/mL) with the highest value of total flavonoid content (TFC, 421.32 mg/g). Moreover, the contents of 8 flavonoids in this fraction were quantified using high-performance liquid chromatography, and fisetin, diosmetin, luteolin, and tectorigenin were the 4 major flavonoids with levels of 31.66, 29.91, 13.69, and 12.41 mg/g, respectively. Luteolin produced a greater inhibition of human lung cancer A549 cells (IC 50 = 59.60 μg/mL) than did fisetin, diosmetin, and tectorigenin. Flow cytometry revealed that the cellular mechanisms of luteolin inhibition of A549 cells were achieved via the induction of cell proliferation arrest at G 1 phase and apoptosis/necrosis. Our findings suggest that flavonoids are closely associated with antitumor, antioxidant, and nitrite scavenging effects of CWCP. © 2016 Institute of Food Technologists®.

  16. Age-stage, two-sex life table of Parapoynx crisonalis (Lepidoptera: Pyralidae) at different temperatures

    PubMed Central

    Chen, Qi; Li, Ni; Wang, Xing; Ma, Li; Huang, Jian-Bin; Huang, Guo-Hua

    2017-01-01

    Parapoynx crisonalis is an important pest of many aquatic vegetables including water chestnuts. Understanding the relationship between temperature variations and the population growth rates of P. crisonalis is essential to predicting its population dynamics in water chestnuts ponds. These relationships were examined in this study based on the age-stage, two-sex life table of P. crisonalis developed in the laboratory at 21, 24, 27, 30, 33 and 36°C. The results showed that the values of Sxj (age-stage–specific survival rate), fxj (age-stage-specific fecundity), lx (age specific survival rate) and mx (age-specific fecundity) increased as the temperature rose from 21 to 27°C, then decreased from 30 to 36°C. Temperature also had a significant effect on the net reproductive rate (R0), gross reproductive rate (GRR), intrinsic rate of increase (r) and finite rate of increase (λ). The value of these parameters were at low levels at 21, 33, and 36°C. Further, the r value decreased as the temperature rose from 24 to 30°C, while the GRR reached its highest level at 27°C. The results indicated that optimal growth and development of P. crisonalis occurred at temperatures between 24°C to 30°C when compared to the lowest temperature (21°C) and higher temperatures of 33°C and 36°C. PMID:28264022

  17. Chemo-enzymatic synthesis of furfuralcohol from chestnut shell hydrolysate by a sequential acid-catalyzed dehydration under microwave and Escherichia coli CCZU-Y10 whole-cells conversion.

    PubMed

    Di, Junhua; Ma, Cuiluan; Qian, Jianghao; Liao, Xiaolong; Peng, Bo; He, Yucai

    2018-08-01

    In this study, chemo-enzymatic synthesis of furfuralcohol from biomass-derived xylose was successfully demonstrated by a sequential acid-catalyzed dehydration under microwave and whole-cells reduction. After dry dewaxed chestnut shells (CNS, 75 g/L) was acid-hydrolyzed with dilute oxalic acid (0.5 wt%) at 140 °C for 40 min, the obtained CNS-derived xylose (17.9 g/L xylose) could be converted to furfural at 78.8% yield with solid acid SO 4 2- /SnO 2 -Attapulgite (2.0 wt% catalyst loading) in the dibutyl phthalate-water (1:1, v:v) under microwave (600 W) at 180 °C for 10 min. In the dibutyl phthalate-water (1:1, v/v) media at 30 °C and pH 6.5, the furfural liquor (47.0 mM furfural) was biologically converted to furfuralcohol by recombinant Escherichia coli CCZU-Y10 whole-cells harboring an NADH-dependent reductase (PgCR) without extra addition of NAD + and glucose, and furfural was completely converted to furfuralcohol after 2.5 h. Clearly, this one-pot synthesis strategy can be effectively used for furfuralcohol production. Copyright © 2018 Elsevier Ltd. All rights reserved.

  18. Effects of plant tannin extracts supplementation on animal performance and gastrointestinal parasites infestation in steers grazing winter wheat

    USDA-ARS?s Scientific Manuscript database

    Twenty-six stocker cattle (286.1 ± 25.7 kg) were used to quantify the effect of commercial plant tannin extracts (control vs. mimosa and chestnut tannins) on animal performance, gastrointestinal parasites control, and plasma metabolite changes in heifers grazing winter wheat forage (Triticum aestivu...

  19. 27 CFR 9.41 - Lancaster Valley.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ....” (b) Approved maps. The appropriate maps for determining the boundaries of the Lancaster Valley... through the town of Gap and along Mine Ridge to the 76°07′30″ west longitude line in Paradise Township. (9... Chestnut Ridge, past Millers Run and continuing until the 400 foot contour line intersects an unnamed...

  20. 27 CFR 9.41 - Lancaster Valley.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ....” (b) Approved maps. The appropriate maps for determining the boundaries of the Lancaster Valley... through the town of Gap and along Mine Ridge to the 76°07′30″ west longitude line in Paradise Township. (9... Chestnut Ridge, past Millers Run and continuing until the 400 foot contour line intersects an unnamed...

  1. Environmental Impact Research Program. Application of the Habitat Evaluation Procedures in Cypress Bayou Basin, Texas.

    DTIC Science & Technology

    1987-04-01

    Species Cypress Cypress Chestnut lamprey Ichthyomyzon castaneus X NC Spotted gar Lepisosteus oculatus 0 NC Longnose gar Lepisosteus osseus X NC Bowfin Amia ... calva 0 0 Gizzard shad Dorosoma cepedianum 0 0 Grass pickerel Esox americanus 0 0 vermiculatus Chain pickerel Esox niger 0 0 Black buffalo Ictiobus

  2. Economic impacts of invasive species in forest past, present, and future

    Treesearch

    Thomas P. Holmes; Juliann E. Aukema; Betsy Von Holle; Andrew Liebhold; Erin Sills

    2009-01-01

    Biological invasions by nonnative species are a by-product of economic activities, with the vast majority of nonnative species introduced by trade and transport of products and people. Although most introduced species are relatively innocuous, a few species ultimately cause irreversible economic and ecological impacts, such as the chestnut blight that functionally...

  3. Unwelcome Guests: Extoic Forest Pests

    Treesearch

    Sun Jiang-Hua

    2002-01-01

    Exotic forest pests cost China and the United States billions of dollars each year. Current regulatory systems worldwide are over-whelmed with the increasing volume of international trade. Trade in nursery stock, wood products, pallets and dunnage have proven the most common means of transport for exotic forest pests. Despite our best efforts, pests such as chestnut...

  4. Utility of wire cages, tree shelters, and repellants to minimize herbivory to oak by white-tailed deer

    Treesearch

    James N. Kochenderfer; W.Mark Ford

    2008-01-01

    We evaluated the efficacy of exclusion cages and commercially available repellants in deterring white-tailed deer (Odocoileus virginianus) herbivory on northern red oak (Quercus rubra) and chestnut oak (Q. montana) stump sprouts and planted red oak seedlings following a commercial clearcut harvest in West...

  5. Effects of inoculum density and wounding on stem infection of three Eastern U.S. forest species by Phytophthora ramorum

    USDA-ARS?s Scientific Manuscript database

    Seedlings of three Eastern US forest species (red maple, northern red oak, and chestnut oak) were inoculated by applying Phytophthora ramorum sporangia to stems at different inoculum densities with and without wounding. Disease occurred in all treatments involving wounds, and no disease was observe...

  6. Effects of inoculum density and wounding on stem infection of three eastern US forest species by Phytophthora ramorum

    Treesearch

    P.W. Tooley; M. Browning; R.M. Leighty

    2014-01-01

    Seedlings of three Eastern US forest species Quercus rubra (northern red oak), Quercus prinus (chestnut oak) and Acer rubrum (red maple) were inoculated by applying Phytophthora ramorum sporangia to stems at different inoculum densities with and without wounding. Disease occurred in all...

  7. Acorn Production Characteristics of Southern Appalachian Oaks: A Simple Method to Predict Within-Year Crop Size

    Treesearch

    Cathryn H. Greenberg; Bernard R. Parresol

    2000-01-01

    We examined acorn production from 1993-97 by black oak (Quercus velutina Lam.), northern red oak (Q. rubra L.), scarlet oak (Q. coccinea Muenchh.), chestnut oak (Q. prinus L.), and white oak (Q. alba L.) in the Southern Appalichians to determine how frequency of acorn...

  8. Ex-situ conservation of Quercus oglethorpensis in living collections of arboreta and botanical gardens.

    Treesearch

    Matthew S. Lobdell; Patrick G. Thompson

    2017-01-01

    Quercus oglethorpensis (Oglethorpe oak) is an endangered species native to the southeastern United States. It is threatened by land use changes, competition, and chestnut blight disease caused by Cryphonectria parasitica. The species is distributed sparsely over a linear distance of ca. 950 km. Its range includes several...

  9. Oak Growth and Response to Thinning

    Treesearch

    Stephen R. Shifley

    2004-01-01

    Oak growth and yield is simultaneously influenced by tree-, stand-, and landscape-scale factors. At the tree scale oak diameter growth varies by tree species (typically n. red oak >= scarlet oak > black oak > white oak > chestnut oak > chinkapin oak > post oak), but oak diameter growth is even more strongly influenced by crown class. Oak stands go...

  10. High-Intensity Fires May Be Unnecessary For Stand Replacement Of Table Mountain Pine: An Overview Of Current Research

    Treesearch

    Thomas A. Waldrop; Patrick H. Brose; Nicole Turrill Welch; Helen H. Mohr; Ellen A. Gray; Frank H. Tainter; Lisa E. Ellis

    2002-01-01

    Abstract - After several decades of fire suppression, ridgetop pine communities of the Southern Appalachians are entering later seral stages and beginning to disappear. They typically have an overstory of Table Mountain pine (Pinus pungens), which is being replaced by shade-tolerant chestnut oaks (Quercus prinus...

  11. Development and characterization of fourteen novel microsatellite markers for the chestnut short-tailed fruit bat (Carollia castanea), and cross-amplification to related species.

    PubMed

    Cleary, Katherine A; Waits, Lisette P; Hohenlohe, Paul A

    2016-01-01

    Rapid anthropogenic land use change threatens the primary habitat of the Chestnut short-tailed bat (Carollia castanea) throughout much of its range. Information on population genetic structure can inform management strategies for this widespread frugivorous bat, and effective protection of C. castanea will also benefit the more than 20 mutualistic plant species of which this bat is the primary seed disperser. To facilitate understanding of population genetic structure in this species, fourteen novel microsatellite markers were developed using restriction-site-associated DNA libraries and Illumina sequencing and tested on 28 individuals from 13 locations in Costa Rica. These are the first microsatellite markers developed for C. castanea. All loci were polymorphic, with number of alleles ranging from 2-11 and average observed heterozygosity of 0.631. Markers were also cross-amplified in three additional frugivorous bat species threatened by habitat loss and fragmentation: Sowell's short-tailed bat (Carollia sowelli), Seba's short-tailed bat (Carollia perspicillata), and the Jamaican fruit bat (Artibeus jamaicensis), and 10, 11, and 8 were polymorphic, respectively.

  12. Effect of infrared and microwave radiations on properties of Indian Horse Chestnut starch.

    PubMed

    Shah, Umar; Gani, Adil; Ashwar, Bilal Ahmad; Shah, Asima; Wani, Idrees Ahmed; Masoodi, Farooq Ahmad

    2016-03-01

    Starch extracted from Indian Horse Chestnut (IHCN) was subjected to infrared and microwave radiations for different time intervals (15 s, 30 s, & 45 s) at constant dose. The structural change of MW and IR radiated IHCN starches were determined by Fourier transform-infra red spectroscopy. The increased peak intensity at 3240 cm(-1) of treated starch represents more exposure of hydroxyl groups due to radiation. Granule morphology of native starch showed round and elliptical granules with smooth surfaces. However radiation treatment resulted in the development of surface cracks. Effect of radiation on physicochemical properties of starch revealed increase in water absorption capacity and light transmittance and decrease in apparent amylose content, pH, and syneresis. The peak, trough, final, and setback viscosities were significantly reduced with increase in treatment time. Radiated starches displayed significantly lower values of To,Tp, and ΔHgel than native starch. Further antioxidant activities were evaluated by DPPH and FRAP assays. Results showed significant improvement in antioxidant activity of starch by both MW and IR treatments. Copyright © 2015 Elsevier B.V. All rights reserved.

  13. Seasonal dynamics and spatial distribution pattern of Parapoynx crisonalis (Lepidoptera: Crambidae) on water chestnuts.

    PubMed

    Li, Ni; Chen, Qi; Zhu, Jie; Wang, Xing; Huang, Jian-Bin; Huang, Guo-Hua

    2017-01-01

    Parapoynx crisonalis (Walker, 1859) (Lepidoptera: Crambidae) is a major pest of aquatic vegetables and aquatic landscape plants. It has been responsible for causing considerable economic damage to water chestnut (Trapa natans) plants. In the Changsha vicinity of China, P. crisonalis has five generations a year. Populations of P. crisonalis were relatively low in April and began to rapidly rise at the beginning of May. At the end of July and early August, the population dropped dramatically. A rebound occurred at the end of August and early September, which was referred to as the second population peak. From then, until early November, the P. crisonalis population steadily diminished in preparation for overwintering. The primary factors influencing the seasonal dynamics of P. crisonalis were the climatic conditions, especially the temperature, and secondarily precipitation. Between May and October, the P. crisonalis adults were evenly distributed in the pond. In May and June, the eggs of P. crisonalis were present in an aggregate distribution, due to the effects of environmental heterogeneity. In July and August, however, they were found to be in a uniform distribution.

  14. Seasonal dynamics and spatial distribution pattern of Parapoynx crisonalis (Lepidoptera: Crambidae) on water chestnuts

    PubMed Central

    Zhu, Jie; Wang, Xing; Huang, Jian-Bin; Huang, Guo-Hua

    2017-01-01

    Parapoynx crisonalis (Walker, 1859) (Lepidoptera: Crambidae) is a major pest of aquatic vegetables and aquatic landscape plants. It has been responsible for causing considerable economic damage to water chestnut (Trapa natans) plants. In the Changsha vicinity of China, P. crisonalis has five generations a year. Populations of P. crisonalis were relatively low in April and began to rapidly rise at the beginning of May. At the end of July and early August, the population dropped dramatically. A rebound occurred at the end of August and early September, which was referred to as the second population peak. From then, until early November, the P. crisonalis population steadily diminished in preparation for overwintering. The primary factors influencing the seasonal dynamics of P. crisonalis were the climatic conditions, especially the temperature, and secondarily precipitation. Between May and October, the P. crisonalis adults were evenly distributed in the pond. In May and June, the eggs of P. crisonalis were present in an aggregate distribution, due to the effects of environmental heterogeneity. In July and August, however, they were found to be in a uniform distribution. PMID:28863164

  15. Effects of Condensed and Hydrolyzable Tannins on Rumen Metabolism with Emphasis on the Biohydrogenation of Unsaturated Fatty Acids.

    PubMed

    Costa, Mónica; Alves, Susana P; Cappucci, Alice; Cook, Shaun R; Duarte, Ana; Caldeira, Rui M; McAllister, Tim A; Bessa, Rui J B

    2018-04-04

    The hypothesis that condensed tannins have higher inhibitory effect on ruminal biohydrogenation than hydrolyzable tannins was tested. Condensed tannin extract from mimosa (CT) and hydrolyzable tannin extract from chestnut (HT) or their mixture (MIX) were incorporated (10%) into oil supplemented diets and fed to rumen fistulated sheep. Fatty acid and dimethyl acetal composition of rumen contents and bacterial biomass were determined. Selected rumen bacteria were analyzed by quantitative real time PCR. Lower ( P < 0.05) rumen volatile fatty acids concentrations were observed with CT compared to HT. Moreover, lower concentration ( P < 0.05) of Fibrobacter succinogenes, Ruminococcus flavefaciens, Ruminococcus albus, and Butyrivibrio proteoclasticus were observed with CT compared to HT. The extension of biohydrogenation of 18:2n-6 and 18:3n-3 did not differ among treatments but was much more variable with CT and MIX than with HT. The trans-/ cis-18:1 ratio in bacterial biomass was higher ( P < 0.05) with HT than CT. Thus, mimosa condensed tannins had a higher inhibitory effect on ruminal metabolism and biohydrogenation than chestnut hydrolyzable tannins.

  16. Effects of short-term anoxia treatment on browning of fresh-cut Chinese water chestnut in relation to antioxidant activity.

    PubMed

    You, Yanli; Jiang, Yueming; Sun, Jian; Liu, Hai; Song, Lili; Duan, Xuewu

    2012-06-01

    The effects of short-term anoxia pre-treatment on browning of fresh-cut Chinese water chestnut (CWC), stored at 4°C, in relation to antioxidant activity were investigated. CWC slices were exposed to pure N 2 for 4h and then stored at 4°C for 18d. Anoxia significantly inhibited browning of CWC slices during storage, accompanied by lower contents of malondialdehyde, H 2 O 2 , and lipoxygenase activity. Furthermore, anoxia induced the activities of superoxide dismutase and ascorbate peroxidase, which could benefit scavenging reactive oxygen species and alleviating lipid peroxidation. In addition, better maintenance of reducing power and free-radical-scavenging activities against α,α-diphenyl-β-picrylhydrazy (DPPH), superoxide anions and hydroxyl was observed in N 2 -treated CWC slices, with higher phenolic compounds and ascorbic acid contents. Collectively, these finds suggest that N 2 pre-treatment enhanced enzymatic and non-enzymatic antioxidant activity in CWC slices, and thereby contributed to alleviating lipid peroxidation and maintenance of storage quality. Copyright © 2011 Elsevier Ltd. All rights reserved.

  17. Agricultural non-point source pollution management in a reservoir watershed based on ecological network analysis of soil nitrogen cycling.

    PubMed

    Xu, Wen; Cai, Yanpeng; Rong, Qiangqiang; Yang, Zhifeng; Li, Chunhui; Wang, Xuan

    2018-03-01

    The Miyun Reservoir plays a pivotal role in providing drinking water for the city of Beijing. In this research, ecological network analysis and scenario analysis were integrated to explore soil nitrogen cycling of chestnut and Chinese pine forests in the upper basin of the Miyun Reservoir, as well as to seek favorable fertilization modes to reduce agricultural non-point source pollution. Ecological network analysis results showed that (1) the turnover time was 0.04 to 0.37 year in the NH 4 + compartment and were 15.78 to 138.36 years in the organic N compartment; (2) the Finn cycling index and the ratio of indirect to direct flow were 0.73 and 11.92 for the chestnut forest model, respectively. Those of the Chinese pine forest model were 0.88 and 29.23, respectively; and (3) in the chestnut forest model, NO 3 - accounted for 96% of the total soil nitrogen loss, followed by plant N (2%), NH 4 + (1%), and organic N (1%). In the Chinese pine forest, NH 4 + accounted for 56% of the total soil nitrogen loss, followed by organic N (34%) and NO 3 - (10%). Fertilization mode was identified as the main factor affecting soil N export. To minimize NH 4 + and NO 3 - outputs while maintaining the current plant yield (i.e., 7.85e0 kg N/year), a fertilization mode of 162.50 kg N/year offered by manure should be adopted. Whereas, to achieve a maximum plant yield (i.e., 3.35e1 kg N/year) while reducing NH 4 + and NO 3 - outputs, a fertilization mode of 325.00 kg N/year offered by manure should be utilized. This research is of wide suitability to support agricultural non-point source pollution management at the watershed scale.

  18. The Success of the Horse-Chestnut Leaf-Miner, Cameraria ohridella, in the UK Revealed with Hypothesis-Led Citizen Science

    PubMed Central

    Pocock, Michael J. O.; Evans, Darren M.

    2014-01-01

    Citizen science is an increasingly popular way of undertaking research and simultaneously engaging people with science. However, most emphasis of citizen science in environmental science is on long-term monitoring. Here, we demonstrate the opportunities provided by short-term hypothesis-led citizen science. In 2010, we ran the ‘Conker Tree Science’ project, in which over 3500 people in Great Britain provided data at a national scale of an insect (horse-chestnut leaf-mining moth, Cameraria ohridella) undergoing rapid range-expansion. We addressed two hypotheses, and found that (1) the levels of damage caused to leaves of the horse-chestnut tree, Aesculus hippocastanum, and (2) the level of attack by parasitoids of C. ohridella larvae were both greatest where C. ohridella had been present the longest. Specifically there was a rapid rise in leaf damage during the first three years that C. ohridella was present and only a slight rise thereafter, while estimated rates of parasitism (an index of true rates of parasitism) increased from 1.6 to 5.9% when the time C. ohridella had been present in a location increased from 3 to 6 years. We suggest that this increase is due to recruitment of native generalist parasitoids, rather than the adaptation or host-tracking of more specialized parasitoids, as appears to have occurred elsewhere in Europe. Most data collected by participants were accurate, but the counts of parasitoids from participants showed lower concordance with the counts from experts. We statistically modeled this bias and propagated this through our analyses. Bias-corrected estimates of parasitism were lower than those from the raw data, but the trends were similar in magnitude and significance. With appropriate checks for data quality, and statistically correcting for biases where necessary, hypothesis-led citizen science is a potentially powerful tool for carrying out scientific research across large spatial scales while simultaneously engaging many people with science. PMID:24465973

  19. The success of the horse-chestnut leaf-miner, Cameraria ohridella, in the UK revealed with hypothesis-led citizen science.

    PubMed

    Pocock, Michael J O; Evans, Darren M

    2014-01-01

    Citizen science is an increasingly popular way of undertaking research and simultaneously engaging people with science. However, most emphasis of citizen science in environmental science is on long-term monitoring. Here, we demonstrate the opportunities provided by short-term hypothesis-led citizen science. In 2010, we ran the 'Conker Tree Science' project, in which over 3500 people in Great Britain provided data at a national scale of an insect (horse-chestnut leaf-mining moth, Cameraria ohridella) undergoing rapid range-expansion. We addressed two hypotheses, and found that (1) the levels of damage caused to leaves of the horse-chestnut tree, Aesculus hippocastanum, and (2) the level of attack by parasitoids of C. ohridella larvae were both greatest where C. ohridella had been present the longest. Specifically there was a rapid rise in leaf damage during the first three years that C. ohridella was present and only a slight rise thereafter, while estimated rates of parasitism (an index of true rates of parasitism) increased from 1.6 to 5.9% when the time C. ohridella had been present in a location increased from 3 to 6 years. We suggest that this increase is due to recruitment of native generalist parasitoids, rather than the adaptation or host-tracking of more specialized parasitoids, as appears to have occurred elsewhere in Europe. Most data collected by participants were accurate, but the counts of parasitoids from participants showed lower concordance with the counts from experts. We statistically modeled this bias and propagated this through our analyses. Bias-corrected estimates of parasitism were lower than those from the raw data, but the trends were similar in magnitude and significance. With appropriate checks for data quality, and statistically correcting for biases where necessary, hypothesis-led citizen science is a potentially powerful tool for carrying out scientific research across large spatial scales while simultaneously engaging many people with science.

  20. Comparative Genome Analysis Provides Insights into the Evolution and Adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum

    PubMed Central

    Green, Sarah; Studholme, David J.; Laue, Bridget E.; Dorati, Federico; Lovell, Helen; Arnold, Dawn; Cottrell, Joan E.; Bridgett, Stephen; Blaxter, Mark; Huitema, Edgar; Thwaites, Richard; Sharp, Paul M.

    2010-01-01

    A recently emerging bleeding canker disease, caused by Pseudomonas syringae pathovar aesculi (Pae), is threatening European horse chestnut in northwest Europe. Very little is known about the origin and biology of this new disease. We used the nucleotide sequences of seven commonly used marker genes to investigate the phylogeny of three strains isolated recently from bleeding stem cankers on European horse chestnut in Britain (E-Pae). On the basis of these sequences alone, the E-Pae strains were identical to the Pae type-strain (I-Pae), isolated from leaf spots on Indian horse chestnut in India in 1969. The phylogenetic analyses also showed that Pae belongs to a distinct clade of P. syringae pathovars adapted to woody hosts. We generated genome-wide Illumina sequence data from the three E-Pae strains and one strain of I-Pae. Comparative genomic analyses revealed pathovar-specific genomic regions in Pae potentially implicated in virulence on a tree host, including genes for the catabolism of plant-derived aromatic compounds and enterobactin synthesis. Several gene clusters displayed intra-pathovar variation, including those encoding type IV secretion, a novel fatty acid biosynthesis pathway and a sucrose uptake pathway. Rates of single nucleotide polymorphisms in the four Pae genomes indicate that the three E-Pae strains diverged from each other much more recently than they diverged from I-Pae. The very low genetic diversity among the three geographically distinct E-Pae strains suggests that they originate from a single, recent introduction into Britain, thus highlighting the serious environmental risks posed by the spread of an exotic plant pathogenic bacterium to a new geographic location. The genomic regions in Pae that are absent from other P. syringae pathovars that infect herbaceous hosts may represent candidate genetic adaptations to infection of the woody parts of the tree. PMID:20419105

  1. Comparative genome analysis provides insights into the evolution and adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum.

    PubMed

    Green, Sarah; Studholme, David J; Laue, Bridget E; Dorati, Federico; Lovell, Helen; Arnold, Dawn; Cottrell, Joan E; Bridgett, Stephen; Blaxter, Mark; Huitema, Edgar; Thwaites, Richard; Sharp, Paul M; Jackson, Robert W; Kamoun, Sophien

    2010-04-19

    A recently emerging bleeding canker disease, caused by Pseudomonas syringae pathovar aesculi (Pae), is threatening European horse chestnut in northwest Europe. Very little is known about the origin and biology of this new disease. We used the nucleotide sequences of seven commonly used marker genes to investigate the phylogeny of three strains isolated recently from bleeding stem cankers on European horse chestnut in Britain (E-Pae). On the basis of these sequences alone, the E-Pae strains were identical to the Pae type-strain (I-Pae), isolated from leaf spots on Indian horse chestnut in India in 1969. The phylogenetic analyses also showed that Pae belongs to a distinct clade of P. syringae pathovars adapted to woody hosts. We generated genome-wide Illumina sequence data from the three E-Pae strains and one strain of I-Pae. Comparative genomic analyses revealed pathovar-specific genomic regions in Pae potentially implicated in virulence on a tree host, including genes for the catabolism of plant-derived aromatic compounds and enterobactin synthesis. Several gene clusters displayed intra-pathovar variation, including those encoding type IV secretion, a novel fatty acid biosynthesis pathway and a sucrose uptake pathway. Rates of single nucleotide polymorphisms in the four Pae genomes indicate that the three E-Pae strains diverged from each other much more recently than they diverged from I-Pae. The very low genetic diversity among the three geographically distinct E-Pae strains suggests that they originate from a single, recent introduction into Britain, thus highlighting the serious environmental risks posed by the spread of an exotic plant pathogenic bacterium to a new geographic location. The genomic regions in Pae that are absent from other P. syringae pathovars that infect herbaceous hosts may represent candidate genetic adaptations to infection of the woody parts of the tree.

  2. Y-12 Groundwater Protection Program Groundwater and Surface Water Sampling and Analysis Plan for Calendar Year 2004

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Elvado Environmental LLC for the Environmental Compliance Department ES&H Division, Y-12 National Security Complex Oak Ridge, Tennessee

    2003-09-30

    This plan provides a description of the groundwater and surface water quality monitoring activities planned for calendar year (CY) 2004 at the U.S. Department of Energy (DOE) Y-12 National Security Complex that will be managed by the Y-12 Groundwater Protection Program (GWPP). Groundwater and surface water monitoring performed by the GWPP during CY 2004 will be in accordance with the following requirements of DOE Order 5400.1: (1) to maintain surveillance of existing and potential groundwater contamination sources; (2) to provide for the early detection of groundwater contamination and determine the quality of groundwater and surface water where contaminants are mostmore » likely to migrate beyond the Oak Ridge Reservation property line; (3) to identify and characterize long-term trends in groundwater quality at Y-12; and (4) to provide data to support decisions concerning the management and protection of groundwater resources. Groundwater and surface water monitoring during CY 2004 will be performed primarily in three hydrogeologic regimes at Y-12: the Bear Creek Hydrogeologic Regime (Bear Creek Regime), the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime), and the Chestnut Ridge Hydrogeologic Regime (Chestnut Ridge Regime). The Bear Creek and East Fork regimes are located in Bear Creek Valley, and the Chestnut Ridge Regime is located south of Y-12 (Figure A.1). Additional surface water monitoring will be performed north of Pine Ridge, along the boundary of the Oak Ridge Reservation (Figure A.1). Modifications to the CY 2004 monitoring program may be necessary during implementation. Changes in programmatic requirements may alter the analytes specified for selected monitoring wells, or wells could be added or removed from the planned monitoring network. All modifications to the monitoring program will be approved by the Y-12 GWPP manager and documented as addenda to this sampling and analysis plan.« less

  3. Y-12 Groundwater Protection Program Groundwater and Surface Water Sampling and Analysis Plan for Calendar Year 2005

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    2004-09-30

    This plan provides a description of the groundwater and surface water quality monitoring activities planned for calendar year (CY) 2005 at the U.S. Department of Energy (DOE) Y-12 National Security Complex (Y-12) that will be managed by the Y-12 Groundwater Protection Program (GWPP). Groundwater and surface water monitoring performed by the GWPP during CY 2005 will be in accordance with DOE Order 540.1 requirements and the following goals: (1) to maintain surveillance of existing and potential groundwater contamination sources; (2) to provide for the early detection of groundwater contamination and determine the quality of groundwater and surface water where contaminantsmore » are most likely to migrate beyond the Oak Ridge Reservation property line; (3) to identify and characterize long-term trends in groundwater quality at Y-12; and (4) to provide data to support decisions concerning the management and protection of groundwater resources. Groundwater and surface water monitoring during CY 2005 will be performed primarily in three hydrogeologic regimes at Y-12: the Bear Creek Hydrogeologic Regime (Bear Creek Regime), the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime), and the Chestnut Ridge Hydrogeologic Regime (Chestnut Ridge Regime). The Bear Creek and East Fork regimes are located in Bear Creek Valley, and the Chestnut Ridge Regime is located south of Y-12 (Figure A.1). Additional surface water monitoring will be performed north of Pine Ridge, along the boundary of the Oak Ridge Reservation (Figure A.1). Modifications to the CY 2005 monitoring program may be necessary during implementation. Changes in programmatic requirements may alter the analytes specified for selected monitoring wells or may add or remove wells from the planned monitoring network. All modifications to the monitoring program will be approved by the Y-12 GWPP manager and documented as addenda to this sampling and analysis plan.« less

  4. DNA fingerprinting and analysis of population structure in the chestnut blight fungus, Cryphonectria parasitica.

    PubMed

    Milgroom, M G; Lipari, S E; Powell, W A

    1992-06-01

    We analyzed DNA fingerprints in the chestnut blight fungus, Cryphonectria parasitica, for stability, inheritance, linkage and variability in a natural population. DNA fingerprints resulting from hybridization with a dispersed moderately repetitive DNA sequence of C. parasitica in plasmid pMS5.1 hybridized to 6-17 restriction fragments per individual isolate. In a laboratory cross and from progeny from a single perithecium collected from a field population, the presence/absence of 11 fragments in the laboratory cross and 12 fragments in the field progeny set segregated in 1:1 ratios. Two fragments in each progeny set cosegregated; no other linkage was detected among the segregating fragments. Mutations, identified by missing bands, were detected for only one fragment in which 4 of 43 progeny lacked a band present in both parents; no novel fragments were detected in any progeny. All other fragments appeared to be stably inherited. Hybridization patterns did not change during vegetative growth or sporulation. However, fingerprint patterns of single conidial isolates of strains EP155 and EP67 were found to be heterogenous due to mutations that occurred during culturing in the laboratory since these strains were first isolated in 1976-1977. In a population sample of 39 C. parasitica isolates, we found 33 different fingerprint patterns with pMS5.1. Most isolates differed from all other isolates by the presence or absence of several fragments. Six fingerprint patterns each occurred twice. Isolates with identical fingerprints occurred in cankers on the same chestnut stems three times; isolates within the other three pairs were isolated from cankers more than 5 m apart. The null hypothesis of random mating in this population could not be rejected if the six putative clones were removed from the analysis. Thus, a rough estimate of the clonal fraction of this population is 6 in 39 isolates (15.4%).

  5. Contribution of flowering trees to urban atmospheric biogenic volatile organic compound emissions

    NASA Astrophysics Data System (ADS)

    Baghi, R.; Helmig, D.; Guenther, A.; Duhl, T.; Daly, R.

    2012-03-01

    Emissions of biogenic volatile organic compounds (BVOC) from urban trees during and after blooming were measured during spring and early summer 2009 in Boulder, Colorado. Air samples were collected onto solid adsorbent cartridges from branch enclosures on the tree species crabapple, horse chestnut, honey locust, and hawthorn. These species constitute ~65 % of the insect-pollinated fraction of the flowering tree canopy (excluding catkin-producing trees) from the street area managed by the City of Boulder. Samples were analyzed for C10-C15 BVOC by thermal desorption and gas chromatography coupled to a flame ionization detector and a mass spectrometer (GC/FID/MS). Identified emissions and emission rates from these four tree species during the flowering phase were found to vary over a wide range. Monoterpene emissions were identified for honey locust, horse chestnut and hawthorn. Sesquiterpene emissions were observed in horse chestnut and hawthorn samples. Crabapple flowers were found to emit significant amounts of benzyl alcohol and benzaldehyde. Floral BVOC emissions increased with temperature, generally exhibiting exponential temperature dependence. Changes in BVOC speciation during and after the flowering period were observed for every tree studied. Emission rates were significantly higher during the blooming compared to the vegetative state for crabapple and honey locust. Total normalized (30 °C) monoterpene emissions from honey locust were higher during flowering (5.26 μg Cg-1 h-1) than after flowering (1.23 μg Cg-1 h-1). The total normalized BVOC emission rate from crabapple (93 μg Cg-1 h-1) during the flowering period is of the same order as isoprene emissions from oak trees, which are among the highest BVOC emissions observed from plants to date. These findings illustrate that during the relatively brief springtime flowering period, floral emissions constitute by far the most significant contribution to the BVOC flux from these tree species, some of which are leafless at this time. Experimental results were integrated into the MEGAN biogenic emission model and simulations were performed to estimate the contribution of floral BVOC emissions to the total urban BVOC flux during the spring flowering period. The floral BVOC emitted during this three-month simulation are equivalent to 11 % of the cumulative monoterpene flux for the Boulder urban area.

  6. Contribution of flowering trees to urban atmospheric biogenic volatile organic compound emissions

    NASA Astrophysics Data System (ADS)

    Baghi, R.; Helmig, D.; Guenther, A.; Duhl, T.; Daly, R.

    2012-04-01

    Emissions of biogenic volatile organic compounds (BVOC) from urban trees during and after blooming were measured during spring and early summer 2009 in Boulder, Colorado. Air samples were collected onto solid adsorbent cartridges from branch enclosures on the following trees: crabapple, horse chestnut, honey locust and hawthorn. These species constitute ~65% of the insect-pollinated fraction of the flowering tree canopy (excluding catkin-producing trees) from the total street area managed by the City of Boulder. Samples were subsequently analyzed for C10 - C15 BVOC by thermal desorption and gas chromatography coupled to a flame ionization detector and a mass spectrometer (GC/FID/MS). Identified emissions and emission rates from these four tree species during the flowering phase were found to vary over a wide range. Monoterpene emissions were identified for honey locust, horse chestnut and hawthorn. Sesquiterpene emissions were observed in horse chestnut and hawthorn samples. Crabapple flowers were found to emit significant amounts of benzyl alcohol and benzaldehyde. Floral BVOC emissions were found to increase with temperature, generally exhibiting exponential temperature dependence. Changes in BVOC speciation during and after the flowering period were observed for every tree studied. Emission rates were significantly higher during the blooming compared to the vegetative state for crabapple and honey locust. Total normalized (30oC) monoterpene emissions from honey locust were 4.3 fold higher during flowering (5.26 μgC g-1h-1) than after flowering (1.23 μgC g-1h-1). The total normalized BVOC emission rate from crabapple (93 μgC g-1h-1) during the flowering period is of the same order as isoprene emissions from oak trees, which are among the highest BVOC emissions observed to date. These findings illustrate that during the relatively brief springtime flowering period, floral emissions constitute by far the most significant contribution to the BVOC flux from these tree species, some of which are leafless at this time. These experimental results were integrated into the MEGAN biogenic emission model and simulations were performed to estimate the contribution of floral BVOC emissions to the total urban BVOC flux during the spring flowering period. The floral BVOC emitted during this three-month simulation constitute eleven percent of the cumulative monoterpene flux for the Boulder urban area.

  7. The Involvement of Thaumatin-Like Proteins in Plant Food Cross-Reactivity: A Multicenter Study Using a Specific Protein Microarray

    PubMed Central

    Palacín, Arantxa; Rivas, Luis A.; Gómez-Casado, Cristina; Aguirre, Jacobo; Tordesillas, Leticia; Bartra, Joan; Blanco, Carlos; Carrillo, Teresa; Cuesta-Herranz, Javier; Bonny, José A. Cumplido; Flores, Enrique; García-Alvarez-Eire, Mar G.; García-Nuñez, Ignacio; Fernández, Francisco J.; Gamboa, Pedro; Muñoz, Rosa; Sánchez-Monge, Rosa; Torres, Maria; Losada, Susana Varela; Villalba, Mayte; Vega, Francisco; Parro, Victor; Blanca, Miguel; Salcedo, Gabriel; Díaz-Perales, Araceli

    2012-01-01

    Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy. PMID:22970164

  8. Wisconsin's forest resources in 2001.

    Treesearch

    John S. Vissage; Gery J. Brand; Manfred E. Mielke

    2003-01-01

    Results of the 2001 annual inventory of Wisconsin show about 15.8 million acres of forest land, more than 21.6 billion cubic feet of live volume on forest land, and nearly 584 million dry tons of all live aboveground tree biomass on timberland. Gypsy moth, forest tent caterpillar, twolined chestnut borer, bronze birch borer, ash yellows, and white pine blister rust...

  9. Wisconsin's forest resources in 2002.

    Treesearch

    John S. Vissage; Gary J. Brand; Manfred E. Mielke

    2004-01-01

    Results of the 2002 annual inventory of Wisconsin show about 16.0 million acres of forest land, over 22.2 billion cubic feet of live volume on forest land, and nearly 598 million dry tons of all live aboveground tree biomass on timberland. Gypsy moth, forest tent caterpillar, twolined chestnut borer, bronze birch borer, ash yellows, and white pine blister rust were...

  10. Effects of a willow overstory on planted seedlings in a bottomland restoration

    Treesearch

    C. J. Dulohery; Randy K. Kolka; M. R. McKevlin

    2000-01-01

    Four bottomland tree species (green ash, bald cypress, water tupelo, and swamp chestnut oak) were planted under four levels of willow canopy (intact canopy, 60% thinned with herbicide, complete control with herbicide, and complete mechanical removal plus control with herbicide). Through age 5, species selection rather than canopy control treatment was the dominant...

  11. Temporal fluctuations and the role of disturbance in disease progression of the sudden oak death epidemic

    Treesearch

    Melina Kozanitas; Todd W. Osmundson; Matteo Garbelotto

    2013-01-01

    With its high host mortality and ability to cause landscape-scale alterations in forest cover and composition, sudden oak death (SOD) (etiological agent Phytophthora ramorum, Stramenopila, Oomycota) mirrors past forest disease epidemics such as Chestnut Blight and Dutch Elm Disease. In contrast with these past epidemics, however, the appearance of...

  12. Rhododendron maximum impacts seed bank composition and richness following Tsuga canadensis loss in riparian forests

    Treesearch

    Tristan M. Cofer; Katherine J. Elliott; Janis K. Bush; Chelcy F. Miniat

    2018-01-01

    Southern Appalachian riparian forests have undergone changes in composition and function from invasive pathogens and pests. Castanea dentata mortality in the 1930s from chestnut blight (Cryphonectria parasitica) and Tsuga canadensis mortality in the 2000s from the hemlock woolly adelgid (Adelges tsugae) have led to the expansion and...

  13. Rating forest stands for gypsy moth defoliation

    Treesearch

    Owen W. Herrick; David A. Gansner; David A. Gansner

    1986-01-01

    The severity of future defoliation can be estimated from the percentages of basal area in oaks (Quercus), black oak (Q. velutina) and chestnut oak (a prinus), and in trees with good crowns, along with the average diameter of the stand. With information on these variables, the defoliation potential of any hardwood forest stand in an approaching gypsy moth (Lymantria...

  14. Crown release increases growth of crop trees

    Treesearch

    Neil I. Lamson; H. Clay Smith; Arlyn W. Perkey; Samuel M. Brock; Samuel M. Brock

    1990-01-01

    Two Appalachian hardwood stands in north-central West Virginia were thinned. The principal species were red oak, yellow-poplar, and chestnut oak. For both stands the site index for northern red oak averaged 75 feet. An areawide thinning using "basal-area control" was applied to a 54-yearold stand while specific crop trees were selected and released in a 12-...

  15. 77 FR 3759 - Notice of Effectiveness of Exempt Wholesale Generator Status

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Effectiveness of Exempt Wholesale Generator Status Docket Nos. Agua Caliente Solar, LLC EG12-1-000 Windpower Partners 1993, L.P EG12-2-000 South Chestnut LLC EG12-3-000 Sandy Creek Energy Associates, L.P..... EG12-4-000 AES New Creek...

  16. A Key for the Identification of Eighteen Common Timbers.

    ERIC Educational Resources Information Center

    Thomas, P. A.

    1991-01-01

    Dichotomous key for 18 woods in common domestic and architectural use in Britain is provided. It is based upon structures visible with the naked eye and a hand-lens. Descriptions of the necessary anatomy and terminology are given. Timbers include yew, pine, spruce, oak, sweet chestnut, elm, ash, teak, cherry, walnut, mahogany, box, beech,…

  17. Teaching the West in the Early American Republic: Old Chestnuts and the Fruits of New Research.

    ERIC Educational Resources Information Center

    Larson, John Lauritz

    2000-01-01

    Contends that the literature on westward expansion during the early republic does not provide a platform for assisting teachers in the development of a coherent story on the rise of the west. Provides suggestions for recovering the truth about westward expansion using the available literature, both current and old. (CMK)

  18. Phytosanitation: A systematic approach to disease prevention

    Treesearch

    Thomas D. Landis

    2013-01-01

    Phytosanitation is not a new concept but has received renewed attention due to the increasing threat of nursery spread Phytophthora ramorum (PRAM), the fungus-like pathogen that causes Sudden Oak Death. This disease has the potental to become the most serious forest pest since white pine blister rust and chestnut blight. Phytosanitation can help prevent the spread of...

  19. Demonstrating the benefits of phytosanitary regulations: the case of ISPM 15

    Treesearch

    Eckehard G. Brockerhoff; Juliann E. Aukema; Joseph F. Cavey; Lynn J. Garrett; Robert A. Haack; Mark Kimberley; Andrew M. Liebhold; Frank L. Lowenstein; Carissa Marasas; Amelia Nuding; Lars Olson; Christa Speekmann; Michael Springborn; Christina Vieglais; James Turner

    2011-01-01

    Invasions of non-indigenous insects and pathogens threaten trees and forest ecosystems worldwide. For example, the arrival and spread of the pathogens causing chestnut blight and Dutch elm disease, along with the bark beetles vectoring the latter, had dramatic effects on North American forests. Despite our improved awareness of the risks associated with biological...

  20. 76 FR 20049 - Notice of Revised Determination on Reconsideration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ... Distribution Center, 100 T.G. Vaughan, Jr. Road, Galax, Virginia On December 3, 2010, the Department of Labor... Company, Inc., T.G. Vaughan Distribution Center, 100 T.G. Vaughan, Jr. Road, Galax, Virginia (TA-W-74,551B... to B.C. Vaughan Factory/Chestnut Creek Veneer Building (TA-W-74,551A) and T.G. Vaughan Distribution...

  1. Key to the larvae of Castanea-feeding Olethreutinae frequently intercepted at U.S. ports-of-entry

    USDA-ARS?s Scientific Manuscript database

    At least six species of olethreutine moths are common pests of chestnut (Castanea spp.) outside of the U.S. Three are native to, or naturalized in the Mediterranean Region of Europe: Pammene fasciana (L.), Cydia splendana (Hübner), and Cydia fagiglandana (Zeller). Three are native to the Far East...

  2. Ecology and Epidemiology of Crimean-Congo Hemorrhagic Fever Virus Transmission in the Republic of Senegal

    DTIC Science & Technology

    1989-02-01

    were found infested on at least one occasion including: 3/13 Red-beaked Hornbills (Tockus erythrorhynchus), 3/6 Long-Tailed Glossy Starlings...Onea capensis Chestnut-bellied Sand-grouse 5 3 2 10 Pterocles exustus Abyssinian Roller 6 1 7 Coracias abyssinica Red-beaked Hornbill 1 3 1 1 2 3 2 13

  3. Amplification of North American Red Oak Microsatellite Markers in European White Oaks and Chinese Chestnut

    Treesearch

    P. R. Aldrich; M. Jagtap; C. H. Michler; J. Romero-Severson

    2003-01-01

    We examined the cross-species amplification success of thirty microsatellite markers developed from North American northern red oak (Quercus rubra) in other members of the family Fagaceae. Sixteen of these markers are newly developed and we report primer sequences and amplification conditions here. Twelve of the thirty (40.0%) red oak markers...

  4. A novel approach to biocontrol: Release of live insect hosts pre-infected with entomopathogenic nematodes.

    PubMed

    Gumus, Arife; Karagoz, Mehmet; Shapiro-Ilan, David; Hazir, Selcuk

    2015-09-01

    As a new application approach, we tested the efficacy of releasing live insect hosts that were pre-infected with entomopathogenic nematodes against insect pests living in cryptic habitats. We hypothesized that the pre-infected hosts could carry the next generation of emerging nematode infective juveniles to hard-to-reach target sites, and thereby facilitate enhanced control in cryptic habitats. Thus, the infected hosts act as "living insect bombs" against the target pest. We tested this approach using two model insect pests: a chestnut tree pest, the goat moth Cossus cossus (Lepidiptera: Cossidae), and a lawn caterpillar, Spodoptera cilium (Lepidoptera: Noctuidae). One pest is considered hard-to-reach via aqueous spray (C. cossus) and the other is more openly exposed in the environment (S. cilium). C. cossus and S. cilium studies were conducted in chestnut logs and Bermudagrass arenas, respectively. The living bomb approach was compared with standard nematode application in aqueous spray and controls (without nematode application); Steinernema carpocapsae (Rize isolate) was used in all experiments. The percentage larval mortality of C. cossus was 86% in the living insect bomb treatment, whereas, all other treatments and controls exhibited less than 4% mortality. The new approach (living bomb) was equally successful as standard aqueous application for the control of S. cilium larvae. Both methods exhibited more than 90% mortality in the turfgrass arena. Our new approach showed an immense potential to control insect pests living in hard-to-reach cryptic habitats. Copyright © 2015 Elsevier Inc. All rights reserved.

  5. Green lumber grade yields from factory grade logs of three oak species

    Treesearch

    Daniel A. Yaussy

    1986-01-01

    Multivariate regression models were developed to predict green board foot yields for the seven common factory lumber grades processed from white, black, and chestnut oak factory grade logs. These models use the standard log measurements of grade, scaling diameter, log length, and proportion of scaling defect. Any combination of lumber grades (such as 1 Common and...

  6. Linking surface-fire behavior, stem heating, and tissue necrosis

    Treesearch

    A.S. Bova; M.B. Dickinson; M.B. Dickinson

    2005-01-01

    Data from 69 experimental, small-plot fires are used to describe relationships among fire intensity, barksurface heat flux, and depth of necrosis in stem tissue for red maple (Acer rubrum L.) and chestnut oak (Quercus prinus L.j. A tetrazolium staining technique was used to determine the depth of necrosis in tree boles subjected to fires with intensities of 20 to 2000...

  7. Growth, survival, and competitive ability of chestnut (Castanea Mill.) seedlings planted across a gradient of light levels

    Treesearch

    Cornelia C. Pinchot; Scott E. Schlarbaum; Stacy L. Clark; Arnold M. Saxton; Ami M. Sharp; Callie J. Schweitzer; Frederick V. Hebard

    2017-01-01

    There has been an increased interest in tree breeding for resistance to exotic pests and pathogens, however relatively little research has focused on the reintroduction of these tree species. Understanding the durability of resistance in field settings and the field performance of improved trees is critical for successful species reintroduction. To evaluate methods for...

  8. Predicting the losses in sawtimber volume and quality from fires in oak-hickory forests.

    Treesearch

    Robert M. Loomis

    1974-01-01

    Presents a method for predicting future sawtimber losses due to fire-caused wounds. Losses are in terms of: (1) lumber value in dollars, (2) volume in board feet, (3) length of defect in feet, and (4) cross sectional area of defect in square inches. The methods apply to northern red, black, scarlet, white and chestnut oaks.

  9. Site characteristics of American chestnut, oak, and hickory witness trees on the Monongahela National Forest, West Virginia

    Treesearch

    Melissa Thomas-Van Gundy; Michael. Strager

    2011-01-01

    Early metes and bounds surveys of the area that would become the Monongahela National Forest in West Virginia were used to create a digital database of corner witness trees. With these electronic and geo-referenced data, physical characteristics were obtained to describe species' locations on the landscape. To characterize the physical environment, variables...

  10. [Butcher's Broom, in the treatment of venous insufficiency].

    PubMed

    Bylka, Wiesława; Kornobis, Joanna

    2005-08-01

    Numerous extracts of the plants, natural compounds and their derivatives, acting on the venous system, including horse-chestnut seed extracts aescin, rutin, troxerutin, diosmin and hesperidine. They have a long tradition in herbal medicine for their venotonic and anti-oedematous properties. This review is concerning Rusci aculeati rhizoma, which recently taken in Poland on the symptoms of chronic venous insufficiency and hemorrhoids.

  11. Weight, volume, and physical properties of major hardwood species in the Southern Appalachian mountains

    Treesearch

    Alexander III Clark; James G. Schroeder

    1985-01-01

    The weight, volume, and physical properties of trees 1 to 20 inches d.h.h. were determined for basswood, blackgum (upland), red maple,yellow-poplar, white oak, sweet birch, black locust, hickory, black oak, chestnut oak, northern red oak, scarlet oak, and white oak in the Southern Appalachian Mountains. Hard hardwoods, soft hardwoods, and individual species equations...

  12. A Device for Comparing Callus Growth Rates in Vitro

    PubMed Central

    Krul, William R.; Combs, Michael

    1975-01-01

    A device to compare the kinetics of callus growth in vitro is described. Changes in volumes of callus grown in scintillation vials were monitored photometrically without removing the sample from the solid support and medium. It is shown that a fiberglass-paper solid support is superior to a plastic foam solid support for the growth of American chestnut callus. PMID:16659126

  13. Wisconsin's forest resources in 2003

    Treesearch

    John S. Vissage; Gary J. Brand; J.E. Cummings-Carlson,

    2005-01-01

    Results of the 2003 annual inventory of Wisconsin show about 15.9 million acres of forest land, over 21.9 billion cubic feet of live volume on forest land, and nearly 591 million dry tons of all live aboveground tree biomass on timberland. Gypsy moth, forest tent caterpillar, twolined chestnut borer, bronze birch borer, ash yellows, and oak wilt were among the pests of...

  14. Photosynthetic Characteristics of Five Hardwood Species in a Mixed Stand

    Treesearch

    Shi-Jean S. Sung; Paul P. Kormanik; Stanley J. Zarnoch

    1999-01-01

    In 1998, photosythesis (Pn) was measured in cherrybark oak, green ash, swamp chestnut oak, sweetgum, and water hickory in a mixed stand established in February 1994. Based on the apparent quantum yield obtained from light response curves, cherrybark oak had the lowest Pn in August whereas sweetgum, green ash, and water hickory were equally active in Pn. Daily August...

  15. Tic-Tac-Toe Performance as a Function of Maturational Level of Retarded Adolescents and Nonretarded Children

    ERIC Educational Resources Information Center

    Spitz, Herman H.; Winters, Emilia A.

    1977-01-01

    Available from: Ablex Publishing Corporation, 355 Chestnut Street, Norwood, New Jersey 07648. Two groups (36 Ss) of educable and trainable mentally retarded adolescents in an institution were compared with two groups (38 Ss) of nonretarded children (ages 8-9 years old) on a modified tic-tac-toe game for foresight in logical problem solving. (MH)

  16. Using fire to restore pine/hardwood ecosystems in the Southern Appalachians of North Carolina

    Treesearch

    James M. Vose; Wayne T. Swank; Barton D. Clinton; Ronald L. Hendrick; Amy E. Major

    1997-01-01

    In the Southern Appalachians, mixed pine/hardwood ecosystems occupy the most xeric sites (i.e., south/west aspect ridge sites). They are typically comprised of varying proportions of pitch pine (Pinus rigida), Virginia pine (Pinus virginiana), and/or shortleaf pine (Pinus echinata) and a mixture of hardwoods, including scarlet oak (Quercus coccinea), chestnut oak (...

  17. Long- and short-term flooding effects on survival and sink-source relationships of swamp-adapted tree species

    Treesearch

    M.N. Angelov; Shi-Jean S. Sung; R.L. Doong; W.R. Harms; Paul P. Kormanik; C.C. Black

    1995-01-01

    About 95% of swamp tupelo (Nyssa sylvatica var. biflora (Walt.) Sarg.) And sweetgum (Liquidambar styraciflua L.) seedlings survived continuous root flooding for more than two years, whereas none of the swamp chestnut oak (Quercus michauxii Nutt.) And cherrybark oak (Q. falcata var. pagodifolia Ell.) Seedlings survived one year of flooding.Flooding caused increases in...

  18. A double row alley-cropping system for establishing nut orchards

    Treesearch

    Jerry Van Sambeek; William. Reid

    2017-01-01

    One of the greatest deterrents to establishing a new nut orchard is the long period of time it takes from tree planting to first commercial nut harvest. At the Pecan Experiment Field, a pecan or walnut must grow ten seasons or more and maybe a little less for Chinese chestnuts before the trees produces enough nuts to warrant mechanical harvesting.

  19. Butt-log grade distributions for five Appalachian hardwood species

    Treesearch

    John R. Myers; Gary W. Miller; Harry V., Jr. Wiant; Joseph E. Barnard; Joseph E. Barnard

    1986-01-01

    Tree quality is an important factor in determining the market value of hardwood timber stands, but many forest inventories do not include estimates of tree quality. Butt-log grade distributions were developed for northern red oak, black oak, white oak, chestnut oak, and yellow-poplar using USDA Forest Service log grades on more than 4,700 trees in West Virginia. Butt-...

  20. Effects of prescribed fire in a central Appalachian oak-hickory stand

    Treesearch

    G.W. Wendel; H. Clay Smith; H. Clay Smith

    1986-01-01

    A prescribed fire in a central Appalachian mixed hardwood stand caused considerable damage to the butt logs of many overstory trees. Although there were increases in the abundance and distribution of several species of hardwoods, advanced red and chestnut oaks were poorly distributed 5-years after burning. An abundance of striped maple and other shrubs in the...

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