50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2011 CFR

2011-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2013 CFR

2013-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2012 CFR

2012-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

50 CFR 226.211 - Critical habitat for Seven Evolutionarily Significant Units (ESUs) of Salmon (Oncorhynchus spp...

Code of Federal Regulations, 2010 CFR

2010-10-01

... form and maintain physical habitat conditions and support juvenile growth and mobility; (ii) Water...); Fossil Creek (39.9447, -123.0403); Middle Fork Eel River (40.0780, -123.0442); North Fork Middle Fork Eel...

Input-form data for the U.S. Geological Survey assessment of the Devonian and Mississippian Bakken and Devonian Three Forks Formations of the U.S. Williston Basin Province, 2013

USGS Publications Warehouse

,; Gaswirth, Stephanie B.; Marra, Kristen R.; Cook, Troy A.; Charpentier, Ronald R.; Gautier, Donald L.; Higley, Debra K.; Klett, Timothy R.; Lewan, Michael D.; Lillis, Paul G.; Schenk, Christopher J.; Tennyson, Marilyn E.; Whidden, Katherine J.

2013-01-01

In 2013, the U.S. Geological Survey assessed the technically recoverable oil and gas resources of the Bakken and Three Forks Formations of the U.S. portion of the Williston Basin. The Bakken and Three Forks Formations were assessed as continuous and hypothetical conventional oil accumulations using a methodology similar to that used in the assessment of other continuous- and conventional-type assessment units throughout the United States. The purpose of this report is to provide supplemental documentation and information used in the Bakken-Three Forks assessment.

15. Potential Transformer for Unit 2 and Operating Floor Front ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. Potential Transformer for Unit 2 and Operating Floor Front Corridor, view to the east-southeast. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

THE FORK AND THE KINASE: A DNA REPLICATION TALE FROM A CHK1 PERSPECTIVE

PubMed Central

González Besteiro, Marina A.; Gottifredi, Vanesa

2014-01-01

Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. The checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged-DNA. Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Such findings unveil a puzzling connection between Chk1 and DNA-lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, the multifaceted and versatile functions of Chk1 at ongoing forks and replication origins determine the extent and quality of the cellular response to replication stress. PMID:25795119

Hydrogeologic framework of the North Fork and surrounding areas, Long Island, New York

USGS Publications Warehouse

Schubert, Christopher E.; Bova, Richard G.; Misut, Paul E.

2004-01-01

Ground water on the North Fork of Long Island is the sole source of drinking water, but the supply is vulnerable to saltwater intrusion and upconing in response to heavy pumping. Information on the area's hydrogeologic framework is needed to analyze the effects of pumping and drought on ground-water levels and the position of the freshwater-saltwater interface. This will enable water-resource managers and water-supply purveyors to evaluate a wide range of water-supply scenarios to safely meet water-use demands. The extent and thickness of hydrogeologic units and position of the freshwater-saltwater interface were interpreted from previous work and from exploratory drilling during this study.The fresh ground-water reservoir on the North Fork consists of four principal freshwater flow systems (referred to as Long Island mainland, Cutchogue, Greenport, and Orient) within a sequence of unconsolidated Pleistocene and Late Cretaceous deposits. A thick glacial-lake-clay unit appears to truncate underlying deposits in three buried valleys beneath the northern shore of the North Fork. Similar glacial-lake deposits beneath eastern and east-central Long Island Sound previously were inferred to be younger than the surficial glacial deposits exposed along the northern shore of Long Island. Close similarities in thickness and upper-surface altitude between the glacial-lake-clay unit on the North Fork and the glacial-lake deposits in Long Island Sound indicate, however, that the two are correlated at least along the North Fork shore.The Matawan Group and Magothy Formation, undifferentiated, is the uppermost Cretaceous unit on the North Fork and constitutes the Magothy aquifer. The upper surface of this unit contains a series of prominent erosional features that can be traced beneath Long Island Sound and the North Fork. Northwest-trending buried ridges extend several miles offshore from areas southeast of Rocky Point and Horton Point. A promontory in the irregular, north-facing cuesta slope extends offshore from an area southwest of Mattituck Creek and James Creek. Buried valleys that trend generally southeastward beneath Long Island Sound extend onshore northeast of Hashamomuck Pond and east of Goldsmith Inlet.An undifferentiated Pleistocene confining layer, the lower confining unit, consists of apparently contiguous units of glacial-lake, marine, and nonmarine clay. This unit is more than 200 feet thick in buried valleys filled with glacial-lake clay along the northern shore, but elsewhere on the North Fork, it is generally less than 50 feet thick and presumably represents an erosional remnant of marine clay. Its upper surface is generally 75 feet or more below sea level where it overlies buried valleys, and is generally 100 feet or less below sea level in areas where marine clay has been identified.A younger unit of glacial-lake deposits, the upper confining unit, is a local confining layer and underlies a sequence of late Pleistocene moraine and outwash deposits. This unit is thickest (more than 45 feet thick) beneath two lowland areas--near Mattituck Creek and James Creek, and near Hashamomuck Pond--but pinches out close to the northern and southern shores and is locally absent in inland areas of the North Fork. Its upper-surface altitude generally rises to near sea level toward the southern shore.Freshwater in the Orient flow system is limited to the upper glacial aquifer above the top of the lower confining unit. The upper confining unit substantially impedes the downward flow of freshwater in inland parts of the Greenport flow system. Deep freshwater within the lower confining unit in the east-central part of the Cutchogue flow system probably is residual from an interval of lower sea level. The upper confining unit is absent or only a few feet thick in the west-central part of the Cutchogue flow system and does not substantially impede the downward flow of freshwater, but the lower confining unit probably impedes the downward flow of freshwater within a southeast-trending buried valley in this area.

Rinne revisited: steel versus aluminum tuning forks.

PubMed

MacKechnie, Cheryl A; Greenberg, Jesse J; Gerkin, Richard C; McCall, Andrew A; Hirsch, Barry E; Durrant, John D; Raz, Yael

2013-12-01

(1) Determine whether tuning fork material (aluminum vs stainless steel) affects Rinne testing in the clinical assessment of conductive hearing loss (CHL). (2) Determine the relative acoustic and mechanical outputs of 512-Hz tuning forks made of aluminum and stainless steel. Prospective, observational. Outpatient otology clinic. Fifty subjects presenting May 2011 to May 2012 with negative or equivocal Rinne in at least 1 ear and same-day audiometry. Rinne test results using aluminum and steel forks were compared and correlated with the audiometric air-bone gap. Bench top measurements using sound-level meter, microphone, and artificial mastoid. Patients with CHL were more likely to produce a negative Rinne test with a steel fork than with an aluminum fork. Logistic regression revealed that the probability of a negative Rinne reached 50% at a 19 dB air-bone gap for stainless steel versus 27 dB with aluminum. Bench top testing revealed that steel forks demonstrate, in effect, more comparable air and bone conduction efficiencies while aluminum forks have relatively lower bone conduction efficiency. We have found that steel tuning forks can detect a lesser air-bone gap compared to aluminum tuning forks. This is substantiated by observations of clear differences in the relative acoustic versus mechanical outputs of steel and aluminum forks, reflecting underlying inevitable differences in acoustic versus mechanical impedances of these devices, and thus efficiency of coupling sound/vibratory energy to the auditory system. These findings have clinical implications for using tuning forks to determine candidacy for stapes surgery.

8. Generator Barrel and Shaft of Unit 1, view to ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. Generator Barrel and Shaft of Unit 1, view to the northwest, with turbine shaft and thrust bearing visible in upper center of photograph. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

11. DETAIL VIEW OF BRIDGE DATEPLATE AT SOUTHEAST CORNER OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. DETAIL VIEW OF BRIDGE DATEPLATE AT SOUTHEAST CORNER OF BRIDGE WHICH READS 'NORTH FORK OF WHITE RIVER, VINCENNES STEEL CORP., CONTRACTOR, ARKANSAS STATE HIGHWAY COMMISSION AND THE UNITED STATES BUREAU OF PUBLIC ROADS, 1936' - North Fork Bridge, Spans North Fork of White River at State Highway 5, Norfork, Baxter County, AR

Archaeological Investigations on the East Fork of the Salmon River, Custer County, Idaho.

DTIC Science & Technology

1984-01-01

coniferous environment in addition to pine marten (Martes americana), red squirrel (Tamiasciurus hudsonicus), porcupine (Erithizon dorsatum), mountain vole...can be seen in small herds throughout the East Fork valley from the Salmon River to Big Boulder Creek. Two bands of Rocky Mountain bighorn sheep...utilize the Challis Planning Unit, one on the East Fork and the other in the Birch Creek area. The East Fork herd is comprised of approximately 50-70

The fork and the kinase: a DNA replication tale from a CHK1 perspective.

PubMed

González Besteiro, Marina A; Gottifredi, Vanesa

2015-01-01

Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. Checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged DNA. Subsequently, Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Indeed, such findings have unveiled a puzzling connection between Chk1 and DNA lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, Chk1 is a multifaceted and versatile signaling factor that acts at ongoing forks and replication origins to determine the extent and quality of the cellular response to replication stress. Copyright © 2014 Elsevier B.V. All rights reserved.

7. Unit 3 Service Water System Valves, view to the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Unit 3 Service Water System Valves, view to the east. These pipes and valves supply water from the draft chest for cooling the generator barrels. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

Peak Metamorphic Temperature Profile across Eastern Belt Franciscan, Northern California Coast Ranges

NASA Astrophysics Data System (ADS)

Schmidt, W. L.; Platt, J. P.

2017-12-01

Previous work done on metamorphic temperatures across the lawsonite-albite to blueschist facies rocks of the Eastern Belt of the Franciscan accretionary complex has relied on a combination of many methods, and suggests that temperature broadly increases from west to east. The Taliaferro Metamorphic Complex is an exception to this pattern and shows higher pressures, and possibly higher temperatures, than its surroundings. The exact location and nature of the faults separating accreted packets in the Eastern Belt is somewhat controversial. A recently calibrated low-temperature laser Raman geothermometer for use on carbonaceous material provides a uniform method of estimating peak metamorphic temperature across the eastern Franciscan and is here used to identify the position of major tectonic boundaries. Temperatures were obtained from exposures in Thomes Creek, Cottonwood Creek, Grindstone Creek, and the middle fork of the Eel River. Peak T in the South Fork Mountain Schist, the highest grade and easternmost unit in the Franciscan, is 310-375°C, whereas in immediately underlying lawsonite-albite facies rocks below the Log Springs thrust, peak T is 270 - 300°C. The Taliaferro Metamorphic Complex reached a peak temperature of 336°C, whereas the surrounding lawsonite-albite facies rocks yield peak temperatures as low as 232°C. Preliminary temperature profiles clearly allow the major faults bounding the Taliaferro Metamorphic Complex and the South Fork Mountain Schist to be located. Extension of the temperature profile has the potential to reveal further detail within these units and the lower grade rocks surrounding them.

16. Governor Accumulator Tanks for Units 3 and 4 and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. Governor Accumulator Tanks for Units 3 and 4 and Grounding Transformer for Unit 4, view to the east. The back of the governor housing is visible in center of photograph, between the accumulator tanks. The grounding transformer for Unit 4 is located on left side of photograph, behind wire mesh safety cage. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

Caspar Creek ecology project: annual report, 1967-68

Treesearch

John W. DeWitt

1968-01-01

Two summers of calibration of the north and south fork Caspar Creek stream ecology study areas were completed in 1967. Clearing for logging road construction in the south fork watershed began in May, 1957. Bulldozer operations first reached the stream itself in July. Some calibration determinations were made during the period of road construction and stream clearance...

26. Generator Voltage Regulator Cabinet Exterior for Unit 1, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. Generator Voltage Regulator Cabinet Exterior for Unit 1, view to the northwest. The exciter supplies the DC current to the generator rotor to create electricity. Each of the four original units has an exciter identical to this one, and all are scheduled for replacement. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

The carpenter fork bed, a new - and older - Black-shale unit at the base of the New Albany shale in central Kentucky: Characterization and significance

USGS Publications Warehouse

Barnett, S.F.; Ettensohn, F.R.; Norby, R.D.

1996-01-01

Black shales previously interpreted to be Late Devonian cave-fill or slide deposits are shown to be much older Middle Devonian black shales only preserved locally in Middle Devonian grabens and structural lows in central Kentucky. This newly recognized - and older -black-shale unit occurs at the base of the New Albany Shale and is named the Carpenter Fork Bed of the Portwood Member of the New Albany Shale after its only known exposure on Carpenter Fork in Boyle County, central Kentucky; two other occurrences are known from core holes in east-central Kentucky. Based on stratigraphic position and conodont biostratigraphy, the unit is Middle Devonian (Givetian: probably Middle to Upper P. varcus Zone) in age and occurs at a position represented by an unconformity atop the Middle Devonian Boyle Dolostone and its equivalents elsewhere on the outcrop belt. Based on its presence as isolated clasts in the overlying Duffin Bed of the Portwood Member, the former distribution of the unit was probably much more widespread - perhaps occurring throughout western parts of the Rome trough. Carpenter Fork black shales apparently represent an episode of subsidence or sea-level rise coincident with inception of the third tectophase of the Acadian orogeny. Deposition, however, was soon interrupted by reactivation of several fault zones in central Kentucky, perhaps in response to bulge migration accompanying start of the tectophase. As a result, much of central Kentucky was uplifted and tilted, and the Carpenter Fork Bed was largely eroded from the top of the Boyle, except in a few structural lows like the Carpenter Fork graben where a nearly complete record of Middle to early Late Devonian deposition is preserved.

Grand Forks - East Grand Forks Urban Water Resources Study. Background Information Appendix.

DTIC Science & Technology

1981-07-01

Social Organization 121 Introduction 121 Demography 122 Racial Distribution 139 Ethnic Groups 141 Neighborhood Groups 141 Religion 143 Crime 144...of agricultural clearing operations and urban growth. Native tree species are important because of their longevity and now relatively infrequent... Demography Geographic Distribution Governmental Organiization Economy Occupational Structure Education Social Support Services 109 CULTURAL ELEM1ENTS

10. Turbine Pit of Unit 5, view to the north. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Turbine Pit of Unit 5, view to the north. Note the difference in configuration within this turbine pit as compared to one of the original pits illustrated in photograph number MT-105-A-11. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

11. Turbine Pit and Shaft of Unit 1, view to ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Turbine Pit and Shaft of Unit 1, view to the south, with operating ring at base of shaft and servo motor arms in foreground and in left background recess. Turbine monitoring and auxiliary equipment is located in the rightbackground recess. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

Appendix 5 Case Studies of Sampling and Monitoring

EPA Science Inventory

The North Fork of Clear Creek (NFCC), Colorado, is an acid-mine drainage impacted stream located approximately 48 km west of Denver, CO, is approximately 29 km long, and flows into the main stem of Clear Creek in Golden, CO. The entire Clear Creek watershed is a Superfund site, ...

Relations between precipitation and daily and monthly mean flows in gaged, unmined and valley-filled watersheds, Ballard Fork, West Virginia, 1999-2001

USGS Publications Warehouse

Messinger, Terence; Paybins, Katherine S.

2003-01-01

Large-scale surface mining using valley fills has changed hydrologic storage and processes in the Ballard Fork Watershed in West Virginia. Total unit flow for the 2-year study period (November 15, 1999?November 14, 2001) on the Unnamed Tributary (extensively mined) (11,700 cubic feet per second per square mile) was almost twice that on Spring Branch (unmined) (6,260 cubic feet per second per square mile), and about 1.75 times that on Ballard Fork (downstream, partly mined) (6,690 cubic feet per second per square mile). Unit flow from the Unnamed Tributary exceeded that from the other two streams for all flows analyzed (5?95 percent duration). Unit flow from Ballard Fork exceeded unit flow from Spring Branch about 80 percent of the time, but was about the same for high flows (less than 20 percent duration). The proportional differences among sites were greatest at low flows. Spring Branch was dry for several days in October and November 2000 and for most of October 2001, and the Unnamed Tributary had flow throughout the study period. The increase in flows from mined parts of the Ballard Fork Watershed appears to result from decreases in evapotranspiration caused by removal of trees and soil during mining. During both years, evapotranspiration from the Spring Branch Watershed greatly exceeded that from the Unnamed Tributary Watershed during May through October, when leaves were open. Evapotranspiration from the Unnamed Tributary Watershed slightly exceeded that from the Spring Branch Watershed in February and March during both years. Evapotranspiration, as a percentage of total rainfall, decreased from the first to the second, drier, year from the Unnamed Tributary Watershed (from 61 percent to 49 percent) but changed little from the Spring Branch (from 77 to 76 percent) and Ballard Fork (73 to 76 percent) Watersheds. Precipitation and flow during the study period at three nearby long-term sites, the U.S. Geological Survey stream-gaging station East Fork Twelvepole Creek near Dunlow, West Virginia, and two National Oceanic Atmospheric Administration rain gages at Madison and Dunlow, West Virginia, were less than long-term annual averages. Relations observed among the three streams in the Ballard Fork Watershed during this study may not represent those in years when annual precipitation and flow are closer to long-term averages.

U.S. Geological Survey 2013 assessment of undiscovered resources in the Bakken and Three Forks Formations of the U.S. Williston Basin Province

USGS Publications Warehouse

Gaswirth, Stephanie B.; Marra, Kristen R.

2014-01-01

The Upper Devonian Three Forks and Upper Devonian to Lower Mississippian Bakken Formations comprise a major United States continuous oil resource. Current exploitation of oil is from horizontal drilling and hydraulic fracturing of the Middle Member of the Bakken and upper Three Forks, with ongoing exploration of the lower Three Forks, and the Upper, Lower, and Pronghorn Members of the Bakken Formation. In 2008, the U.S. Geological Survey (USGS) estimated a mean of 3.65 billion bbl of undiscovered, technically recoverable oil resource within the Bakken Formation. The USGS recently reassessed the Bakken Formation, which included an assessment of the underlying Three Forks Formation. The Pronghorn Member of the Bakken Formation, where present, was included as part of the Three Forks assessment due to probable fluid communication between reservoirs. For the Bakken Formation, five continuous and one conventional assessment units (AUs) were defined. These AUs are modified from the 2008 AU boundaries to incorporate expanded geologic and production information. The Three Forks Formation was defined with one continuous and one conventional AU. Within the continuous AUs, optimal regions of hydrocarbon recovery, or “sweet spots,” were delineated and estimated ultimate recoveries were calculated for each continuous AU. Resulting undiscovered, technically recoverable resource estimates were 3.65 billion bbl for the five Bakken continuous oil AUs and 3.73 billion bbl for the Three Forks Continuous Oil AU, generating a total mean resource estimate of 7.38 billion bbl. The two conventional AUs are hypothetical and represent a negligible component of the total estimated resource (8 million barrels of oil).

A complex mechanism determines polarity of DNA replication fork arrest by the replication terminator complex of Bacillus subtilis.

PubMed

Duggin, Iain G; Matthews, Jacqueline M; Dixon, Nicholas E; Wake, R Gerry; Mackay, Joel P

2005-04-01

Two dimers of the replication terminator protein (RTP) of Bacillus subtilis bind to a chromosomal DNA terminator site to effect polar replication fork arrest. Cooperative binding of the dimers to overlapping half-sites within the terminator is essential for arrest. It was suggested previously that polarity of fork arrest is the result of the RTP dimer at the blocking (proximal) side within the complex binding very tightly and the permissive-side RTP dimer binding relatively weakly. In order to investigate this "differential binding affinity" model, we have constructed a series of mutant terminators that contain half-sites of widely different RTP binding affinities in various combinations. Although there appeared to be a correlation between binding affinity at the proximal half-site and fork arrest efficiency in vivo for some terminators, several deviated significantly from this correlation. Some terminators exhibited greatly reduced binding cooperativity (and therefore have reduced affinity at each half-site) but were highly efficient in fork arrest, whereas one terminator had normal affinity over the proximal half-site, yet had low fork arrest efficiency. The results show clearly that there is no direct correlation between the RTP binding affinity (either within the full complex or at the proximal half-site within the full complex) and the efficiency of replication fork arrest in vivo. Thus, the differential binding affinity over the proximal and distal half-sites cannot be solely responsible for functional polarity of fork arrest. Furthermore, efficient fork arrest relies on features in addition to the tight binding of RTP to terminator DNA.

Base flow (1966-2009) and streamflow gain and loss (2010) of the Brazos River from the New Mexico-Texas State line to Waco, Texas

USGS Publications Warehouse

Baldys, Stanley; Schalla, Frank E.

2012-01-01

Streamflow was measured at 66 sites from June 6–9, 2010, and at 68 sites from October 16–19, 2010, to identify reaches in the upper Brazos River Basin that were gaining or losing streamflow. Gaining reaches were identified in each of the five subbasins. The gaining reach in the Salt Fork Brazos River Basin began at USGS streamflow-gaging station 08080940 Salt Fork Brazos River at State Highway 208 near Clairemont, Tex. (site SF–6), upstream from where Duck Creek flows into the Salt Fork Brazos River and continued downstream past USGS streamflow-gaging station 08082000 Salt Fork Brazos River near Aspermont, Tex. (site SF–9), to the outlet of the basin. In the Double Mountain Fork Brazos River Basin, a gaining reach from near Post, Tex., downstream to the outlet of the basin was identified. Two gaining reaches were identified in the Clear Fork Brazos River Basin—one from near Roby, Tex., downstream to near Noodle, Tex., and second from Hawley, Tex., downstream to Nugent, Tex. Most of the North Bosque River was characterized as gaining streamflow. Streamflow gains were identified in the main stem of the Brazos River from where the Brazos River main stem forms at the confluence of the Salt Fork Brazos River and Double Mountain Fork Brazos River near Knox City, Tex., downstream to near Seymour, Tex.

4. Unit 4 Turbine Pit Oil Jacking Pump and Wicket ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Unit 4 Turbine Pit Oil Jacking Pump and Wicket Gate Linkages, view to the north. The jacking pump, located along the wall on the left side of photograph, is used for pumping oil to lift the thrust bearing prior to starting the unit. Note the wicket gate linkages attached to the operating ring and visible in the lower center of the photograph. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

Grand Forks - East Grand Forks Urban Water Resources Study. Social and Environmental Inventory.

DTIC Science & Technology

1979-01-01

61 Introduction 57IPrehistoric 58 Historic 58-61 Social Organization 62-72 Introduction 62 Demography 62-63 Population Distribution by Age 64-65 Urban...programs, native timber is decreasing due to agricultural clearing operatings and urban growth. Native tree species are important because of their longevity ... Demography 62-63 Population Distribution by Age 64-65 Urban-Rural Distribution 66 Population Projections 67-68 Racial Distribution 69 Ethnic Groups 70

BRCA2 and RAD51 promote double-strand break formation and cell death in response to gemcitabine.

PubMed

Jones, Rebecca M; Kotsantis, Panagiotis; Stewart, Grant S; Groth, Petra; Petermann, Eva

2014-10-01

Replication inhibitors cause replication fork stalling and double-strand breaks (DSB) that result from processing of stalled forks. During recovery from replication blocks, the homologous recombination (HR) factor RAD51 mediates fork restart and DSB repair. HR defects therefore sensitize cells to replication inhibitors, with clear implications for cancer therapy. Gemcitabine is a potent replication inhibitor used to treat cancers with mutations in HR genes such as BRCA2. Here, we investigate why, paradoxically, mutations in HR genes protect cells from killing by gemcitabine. Using DNA replication and DNA damage assays in mammalian cells, we show that even short gemcitabine treatments cause persistent replication inhibition. BRCA2 and RAD51 are recruited to chromatin early after removal of the drug, actively inhibit replication fork progression, and promote the formation of MUS81- and XPF-dependent DSBs that remain unrepaired. Our data suggest that HR intermediates formed at gemcitabine-stalled forks are converted into DSBs and thus contribute to gemcitabine-induced cell death, which could have implications for the treatment response of HR-deficient tumors. ©2014 American Association for Cancer Research.

BRCA2 and RAD51 promote double-strand break formation and cell death in response to Gemcitabine

PubMed Central

Jones, Rebecca M.; Kotsantis, Panagiotis; Stewart, Grant S.; Groth, Petra; Petermann, Eva

2014-01-01

Replication inhibitors cause replication fork stalling and double-strand breaks (DSBs) that result from processing of stalled forks. During recovery from replication blocks, the homologous recombination (HR) factor RAD51 mediates fork restart and DSB repair. HR defects therefore sensitise cells to replication inhibitors, with clear implications for cancer therapy. Gemcitabine is a potent replication inhibitor used to treat cancers with mutations in HR genes such as BRCA2. Here we investigate why, paradoxically, mutations in HR genes protect cells from killing by Gemcitabine. Using DNA replication and -damage assays in mammalian cells, we show that even short Gemcitabine treatments cause persistent replication inhibition. BRCA2 and RAD51 are recruited to chromatin early after removal of the drug, actively inhibit replication fork progression and promote the formation of MUS81- and XPF-dependent DSBs that remain unrepaired. Our data suggest that HR intermediates formed at Gemcitabine-stalled forks are converted into DSBs and thus contribute to Gemcitabine-induced cell death, which could have implications for the treatment response of HR-deficient tumours. PMID:25053826

Production of Artificial Lightning in An Ordinary Clear Light Bulb.

ERIC Educational Resources Information Center

Zaffo, Peter Alfred

1981-01-01

Reported is a method of producing artificial lightning in an ordinary clear lightbulb. The appearance of sparks produced is that of a miniature stroke of forked lightning seen in natural thunderstorms. The sparks also show the intricate branching patterns often seen in natural lightning. (JT)

The distribution of early recombination nodules on zygotene bivalents from plants.

PubMed Central

Anderson, L K; Hooker, K D; Stack, S M

2001-01-01

Early recombination nodules (ENs) are protein complexes approximately 100 nm in diameter that are associated with forming synaptonemal complexes (SCs) during leptotene and zygotene of meiosis. Although their functions are not yet clear, ENs may have roles in synapsis and recombination. Here we report on the frequency and distribution of ENs in zygotene SC spreads from six plant species that include one lower vascular plant, two dicots, and three monocots. For each species, the number of ENs per unit length is higher for SC segments than for (asynapsed) axial elements (AEs). In addition, EN number is strongly correlated with SC segment length. There are statistically significant differences in EN frequencies on SCs between species, but these differences are not related to genome size, number of chromosomes, or phylogenetic class. There is no difference in the frequency of ENs per unit length of SC from early to late zygotene. The distribution of distances between adjacent ENs on SC segments is random for all six species, but ENs are found at synaptic forks more often than expected for a random distribution of ENs on SCs. From these observations, we conclude that in plants: (1) some ENs bind to AEs prior to synapsis, (2) most ENs bind to forming SCs at synaptic forks, and (3) ENs do not bind to already formed SCs. PMID:11729167

1. Pipe Floor Rear Corridor, view to the southeast. The ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Pipe Floor Rear Corridor, view to the southeast. The wall of Unit 2 turbine pit is visible in the right foreground. The pipe and valve cluster in the right foreground is part of the blow down valve for Unit 2. This valve allows the water in the draft chest to be lowered (i.e., 'blown down') so that the unit can be motored (i.e., run like an electric motor rather than an electric power generator). - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

Cultural Resource Investigation of the Grand Forks/East Grand Forks Urban Study and the East Grand Forks Flood Control Project,

DTIC Science & Technology

1981-04-01

and will not be impacted. Remarks: Level II. Father William Sherman, Pastor of St. Michael’s Church can be reached at St. Michael’s Church, 520 North...Trygg, J. William 1967 Composite Map of the Uni- % [ted States Land Surveyors’ L Original Plats and Field Notes, Minnesota Series, Sheet 20, Ely...Investigators/Years: Surveyors’ Original Plats and Notes, ’ 1872 Report/Reference: Trygg, J. William * 1967 Composite Map of United States Surveyors

"Forked Tongue: The Politics of Bilingual Education," by Rosalie Pedalino Porter. Book Review.

ERIC Educational Resources Information Center

Amabisca, Anastasia Aimee

1994-01-01

Based on personal experience rather than empirical evidence, "Forked Tongue" presents a biased view that could damage the future of bilingual education in the United States. The book considers bilingual education a threat because it prolongs the learning of English and promotes the maintenance of other "un-American" languages…

11. Station Accumulator Tanks, view to the northeast. The tanks ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Station Accumulator Tanks, view to the northeast. The tanks are visible along the right side of photograph, opposite a wall of the Unit 1 turbine pit. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

Recombinase and translesion DNA polymerase decrease the speed of replication fork progression during the DNA damage response in Escherichia coli cells

PubMed Central

Tan, Kang Wei; Pham, Tuan Minh; Furukohri, Asako; Maki, Hisaji; Akiyama, Masahiro Tatsumi

2015-01-01

The SOS response is a DNA damage response pathway that serves as a general safeguard of genome integrity in bacteria. Extensive studies of the SOS response in Escherichia coli have contributed to establishing the key concepts of cellular responses to DNA damage. However, how the SOS response impacts on the dynamics of DNA replication fork movement remains unknown. We found that inducing the SOS response decreases the mean speed of individual replication forks by 30–50% in E. coli cells, leading to a 20–30% reduction in overall DNA synthesis. dinB and recA belong to a group of genes that are upregulated during the SOS response, and encode the highly conserved proteins DinB (also known as DNA polymerase IV) and RecA, which, respectively, specializes in translesion DNA synthesis and functions as the central recombination protein. Both genes were independently responsible for the SOS-dependent slowdown of replication fork progression. Furthermore, fork speed was reduced when each gene was ectopically expressed in SOS-uninduced cells to the levels at which they are expressed in SOS-induced cells. These results clearly indicate that the increased expression of dinB and recA performs a novel role in restraining the progression of an unperturbed replication fork during the SOS response. PMID:25628359

77 FR 63311 - Lake Clementine Hydro, LLC; Notice of Successive Preliminary Permit Application Accepted for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-16

... Dam on the North Fork of the American River, near the City of Auburn, Placer County, California. The... Fork Dam, Lake Clementine Hydro plans to install two 7.5 megawatt (MW) generation units, for a total... to study three alternative designs for the project. Applicant Contact: Mr. Magnus Johannesson...

Estimating Sedimentation from an Erosion-Hazard Rating

Treesearch

R.M. Rice; S.A. Sherbin

1977-01-01

Data from two watersheds in northern California were used to develop an interpretation of the erosion hazard rating (EHR) of the Coast Forest District as amount of sedimentation. For the Caspar Creek Experimental Watershed (North Fork and South Fork), each EHR unit was estimated as equivalent to 0.0543 cubic yards per acre per year, on undisturbed forest. Experience...

Estimating sedimentation from an erosion-hazard rating

Treesearch

R. M. Rice; S. A. Sherbin

1977-01-01

Data from two watersheds in northern California were used to develop an interpretation of the erosion-hazard rating (EHR) of the Coast Forest District as amount of sedimentation. For the Caspar Creek Experimental Watershed (North Fork and South Fork), each EHR unit was estimated as equivalent to 0.0543 cubic yards per acre per year, on undisturbed forest. Experience...

5. Station Unwatering Pumps and Sump Pump for Units 1 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Station Unwatering Pumps and Sump Pump for Units 1 and 2, view to the west. The unwatering pumps are the two larger items toward the right side of the photograph (one in foreground and one in background. The smaller item toward the left of the photograph is the sump pump. These pumps are used for draining water from the draft chest for maintenance. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

Viscosity of thickened fluids that relate to the Australian National Standards.

PubMed

Karsten Hadde, Enrico; Ann Yvette Cichero, Julie; Michael Nicholson, Timothy

2016-08-01

In 2007, Australia published standardized terminology and definitions for three levels of thickened fluids used in the management of dysphagia. This study examined the thickness of the current Australian National Fluid Standards rheologically (i.e. viscosity, yield stress) and correlated these results with the "fork test", as described in the national standards. Clinicians who prescribe or work with thickened liquids and laypersons were recruited to categorize 15 different thickened fluids of known viscosities using the fork test. The mean apparent viscosity and the yield stress for each fluid category were calculated. Clear responses were obtained by both clinicians and laypersons for very thin fluids (< 90 mPa.s) and very thick fluids (> 1150 mPa.s), but large variations of responses were seen for intermediate viscosities. Measures of viscosity and yield stress were important in allocating liquids to different categories. Three bands of fluid viscosity with distinct intermediate band gaps and associated yield stress measures were clearly identifiable and are proposed as objective complements to the Australian National Standards. The "fork test" provides rudimentary information about both viscosity and yield stress, but is an inexact measure of both variables.

Two-dimensional resistivity investigation along West Fork Trinity River, Naval Air Station-Joint Reserve Base, Carswell Field, Fort Worth, Texas, October 2004

USGS Publications Warehouse

Shah, Sachin D.; Stanton, Gregory P.

2006-01-01

Naval Air Station-Joint Reserve Base Carswell Field (NAS-JRB) at Fort Worth, Tex., constitutes a government-owned, contractor-operated facility that has been in operation since 1942. Contaminants, primarily volatile organic compounds and metals, have entered the ground-water-flow system through leakage from waste-disposal sites and manufacturing processes. Ground water flows from west to east toward the West Fork Trinity River. During October 2004, the U.S. Geological Survey conducted a two-dimensional (2D) resistivity investigation at a site along the West Fork Trinity River at the eastern boundary of NAS-JRB to characterize the distribution of subsurface resistivity. Five 2D resistivity profiles were collected, which ranged from 500 to 750 feet long and extended to a depth of 25 feet. The Goodland Limestone and the underlying Walnut Formation form a confining unit that underlies the alluvial aquifer. The top of this confining unit is the top of bedrock at NAS-JRB. The bedrock confining unit is the zone of interest because of the potential for contaminated ground water to enter the West Fork Trinity River through saturated bedrock. The study involved a capacitively-coupled resistivity survey and inverse modeling to obtain true or actual resistivity from apparent resistivity. The apparent resistivity was processed using an inverse modeling software program. The results of this program were used to generate distributions (images) of actual resistivity referred to as inverted sections or profiles. The images along the five profiles show a wide range of resistivity values. The two profiles nearest the West Fork Trinity River generally showed less resistivity than the three other profiles.

22. Blow Down Valve for Unit 1, view to the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. Blow Down Valve for Unit 1, view to the southwest. This valve allows the water in the draft chest to be lowered (i.e., 'blown down') so that the unit can be motored (i.e., run like an electric motor rather than an electric power generator). The valve is operated by pressure from the instrument air system (part of which is visible in photograph MT-105-A-17 above), but the unit draws on the station air system (see photograph MT-105-A-24 below) to lower the water in the draft chest. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

Lower Paleozoic deep-water facies of the Medfra area, central Alaska: A section in Geologic studies in Alaska by the U.S. Geological Survey, 1997

USGS Publications Warehouse

Dumoulin, Julie A.; Bradley, Dwight C.; Harris, Anita G.; Repetski, John E.

1999-01-01

Deep-water facies, chiefly hemipelagic deposits and turbidites, of Cambrian through Devonian age are widely exposed in the Medfra and Mt. McKinley quadrangles. These strata include the upper part of the Telsitna Formation (Middle-Upper Ordovician) and the Paradise Fork Formation (Lower Silurian-Lower Devonian) in the Nixon Fork terrane, the East Fork Hills Formation (Upper Cambrian-Lower Devonian) in the East Fork subterrane of the Minchumina terrane, and the chert and argillite unit (Ordovician) and the argillite and quartzite unit (Silurian- Devonian? and possibly older) in the Telida subterrane of the Minchumina terrane.In the western part of the study area (Medfra quadrangle), both hemipelagic deposits and turbidites are largely calcareous and were derived from the Nixon Fork carbonate platform. East- ern exposures (Mt. McKinley quadrangle; eastern part of the Telida subterrane) contain much less carbonate; hemipelagic strata are mostly chert, and turbidites contain abundant rounded quartz and lesser plagioclase and potassium feldspar. Deep-water facies in the Medfra quadrangle correlate well with rocks of the Dillinger terrane exposed to the south (McGrath quadrangle), but coeval strata in the Mt. McKinley quadrangle are compositionally similar to rocks to the northeast (Livengood quadrangle). Petrographic data thus suggest that the Telida subterranes presently defined is an artificial construct made up of two distinct sequences of disparate provenance.Restoration of 90 and 150 km of dextral strike-slip on the Iditarod and Farewell faults, respectively, aligns the deep-water strata of the Minchumina and Dillinger terranes in a position east of the Nixon Fork carbonate platform. This restoration supports the interpretation that lower Paleozoic rocks in the Nixon Fork and Dillinger terranes, and in the western part of the Minchumina terrane (East Fork subterrane and western part of the Telida subterrane), formed along a single continental margin. Rocks in the eastern part of the Telida subterrane are compositionally distinct from those to the west and may have had a different origin and history.

20. Station Unwatering Pumps and Sump Pump, view to the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. Station Unwatering Pumps and Sump Pump, view to the north. The station unwatering pumps are the two large units in the center and right foreground of photograph and are marked with the numbers 1 and 2. The sump pump is the smaller unit in left foreground of photograph. These pumps are used for unwatering the draft chests for maintenance. Note the draft tube unwatering valve visible in background between the two unwatering pumps. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

Integrating conservation genetic considerations into conservation planning: a case study of bull trout in the Lake Pend Oreille-lower Clark Fork River system

Treesearch

John Epifanio; Gordon Haas; Karen Pratt; Bruce Rieman; Paul Spruell; Craig Stockwell; Fred Utter; William Young

2003-01-01

Bull trout (Salvelinus confluentus) is a species of conservation concern-listed as "threatened" under the Endangered Species Act-throughout its native range in the western United States. The authors were assembled by the Clark Fork River Aquatic Implementation Team, composed of biologists representing Montana Fish, Wildlife and Parks (MFWP); Idaho Department...

EFFECTS OF RESOURCE DEVELOPMENT ON WATER QUALITY IN THE BIG SOUTH FORK NATIONAL RIVER AND RECREATION AREA, TENNESSEE AND KENTUCKY.

USGS Publications Warehouse

Carey, William P.; ,

1984-01-01

The South Fork Cumberland River begins in Tennessee at the confluence of the New River and Clear Fork. Strip mining for coal in the New River basin has been ongoing for decades with little reclamation prior to 1977. Water-quality data show that suspended-sediment and dissolved-constituent loads from the New River dominate the water quality in the National River and Recreation Area. The suspended sediment can impart a highly turbid and aesthetically displeasing appearance to the water during low-flow periods which are times of maximum recreational use. High suspended-sediment concentrations are also potentially harmful to the aquatic habitat in the Recreation Area. In addition to the suspended-sediment load, a large supply of coarse material is slowly moving through the channels of the New River basin toward the Recreation Area.

Evaluation of the Life History of Native Salmonids in the Malheur River Basin; Cooperative Bull Trout/Redband Trout Research Project, 1999-2000 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schwabe, Lawrence; Tiley, Mark; Perkins, Raymond R.

2000-11-01

The purpose of this study is to document the seasonal distribution of adult/sub-adult bull trout (Salvelinus confluentus) in the Malheur River basin. Due to the decline of bull trout in the Columbia Basin, the United States Fish and Wildlife Service listed bull trout as a threatened species in June 1998. Past land management activities; construction of dams; and fish eradication projects in the North Fork and Middle Fork Malheur River by poisoning have worked in concert to cumulatively impact native species in the Malheur Basin (Bowers et. al. 1993). Survival of the remaining bull trout populations is severely threatened (Buchananmore » 1997). 1999 Research Objects are: (1) Document the migratory patterns of adult/sub-adult bull trout in the North Fork Malheur River; (2) Determine the seasonal bull trout use of Beulah Reservoir and bull trout entrainment; and (3) Timing and location of bull trout spawning in the North Fork Malheur River basin. The study area includes the Malheur basin from the mouth of the Malheur River located near Ontario, Oregon to the headwaters of the North Fork Malheur River (Map 1). All fish collected and most of the telemetry effort was done on the North Fork Malheur River subbasin (Map 2). Fish collection was conducted on the North Fork Malheur River at the tailwaters of Beulah Reservoir (RK 29), Beulah Reservoir (RK 29-RK 33), and in the North Fork Malheur River at Crane Crossing (RK 69) to the headwaters of the North Fork Malheur. Radio telemetry was done from the mouth of the Malheur River in Ontario, Oregon to the headwaters of the North Fork Malheur. This report will reflect all migration data collected from 3/1/99 to 12/31/99.« less

Late Early Permian continental ichnofauna from Lake Kemp, north-central Texas, USA

USGS Publications Warehouse

Lucas, S.G.; Voigt, S.; Lerner, A.J.; Nelson, W.J.

2011-01-01

Continental trace fossils of Early Permian age are well known in the western United States from Wolfcampian (~. Asselian to Artinskian) strata, but few examples are known from Leonardian (~. Kungurian) deposits. A substantial ichnofauna from strata of the lower part of the Clear Fork Formation at Lake Kemp, Baylor County, Texas, augments the meager North American record of Leonardian continental trace fossil assemblages. Ichnofossils at Lake Kemp occur in the informally-named Craddock dolomite member of the Clear Fork Formation, which is 12-15. m above the local base of the Clear Fork. The trace-bearing stratum is an up-to-0.3. m thick, laminated to flaser-bedded, dolomitic siltstone that also contains mud cracks, raindrop impressions, microbially induced mat structures, and some land-plant impressions. We interpret the Craddock dolomite member as the feather-edge of a marine transgressive carbonate deposit of an irregular coastline marked by shallow bays or estuaries on the eastern shelf of the Midland basin, and the trace-fossil-bearing stratum at Lake Kemp is an unchannelized flow deposit on a muddy coastal plain. The fossil site at Lake Kemp yields a low to moderately diverse fauna of invertebrate and vertebrate traces. A sparse invertebrate ichnofauna consists of arthropod feeding and locomotion traces assigned to Walpia cf. W. hermitensis White, 1929 and Diplichnites gouldi Gevers in Gevers et al., 1971. Tetrapod footprints are most common and assigned to Batrachichnus salamandroides (Geinitz, 1861), cf. Amphisauropus kablikae (Geinitz and Deichm??ller, 1882), and Dromopus lacertoides (Geinitz, 1861), which represent small temnospondyl, seymouriamorph, and basal sauropsid trackmakers. Both the traces and sedimentary features of the fossil horizon indicate a freshwater setting at the time of track formation, and the trace assemblage represents the Scoyenia ichnofacies and the Batrachichnus ichnofacies in an overbank environment with sheet flooding and shallow ephemeral pools on an extensive coastal plain. The Lake Kemp tetrapod track assemblage is characteristic of the global Early Permian tetrapod ichnofauna found in red beds, which is dominated by a handful of ichnogenera that include Batrachichnus, Limnopus, Amphisauropus, Dromopus, Varanopus, Hyloidichnus, Ichniotherium and Dimetropus, which are the tracks of temnospondyls, seymouriamorphs, diadectomorphs, "pelycosaurs", "captorhinomorphs", and araeoscelids. The Lake Kemp tracks also further document the continuity of the ichnogenera Batrachichnus, Amphisauropus and Dromopus from Wolfcampian into Leonardian time and thus support the concept that Wolfcampian and Leonardian red-bed tetrapod footprints represent a single biostratigraphic assemblage. ?? 2011 Elsevier B.V.

Channelization and floodplain forests: Impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

USGS Publications Warehouse

Oswalt, S.N.; King, S.L.

2005-01-01

We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased overbank flooding, a rise in the groundwater table, and ponding of upstream timber. Our objectives were to: (1) determine the composition of floodplain vegetation communities along the degraded river reach, (2) to isolate relationships among these communities, geomorphic features, and environmental variables and (3) evaluate successional changes based on current stand conditions. Vegetation communities were not specifically associated with predefined geomorphic features; nevertheless, hydrologic and geomorphic processes as a result of channelization have clearly affected vegetation communities. The presence of valley plugs and continued degradation of upstream reaches and tributaries on the impacted study reach has arrested recovery of floodplain plant communities. Historically common species like Liquidambar styraciflua L. and Quercus spp. L. were not important, with importance values (IV) less than 1, and occurred in less than 20% of forested plots, while Acer rubrum L., a disturbance-tolerant species, was the most important species on the site (IV = 78.1) and occurred in 87% of forested plots. The results of this study also indicate that channelization impacts on the Middle Fork Forked Deer River are more temporally and spatially complex than previously described for other river systems. Rehabilitation of this system necessitates a long-term, landscape-scale solution that addresses watershed rehabilitation in a spatially and temporally hierarchical manner. ?? 2005 Elsevier B.V. All rights reserved.

Assessing the potential for rainbow trout reproduction in tributaries of the Mountain Fork River below Broken Bow Dam, southeastern Oklahoma

Treesearch

James M. Long; Trevor A. Starks; Tyler Farling; Robert Bastarache

2016-01-01

Stocked trout (Salmonidae) in reservoir tailwater systems in the Southern United States have been shown to use tributary streams for spawning and rearing. The lower Mountain Fork of the Little River below Broken Bow Dam is one of two year-round tailwater trout fisheries in Oklahoma, and the only one with evidence of reproduction by stocked rainbow trout (Oncorhynchus...

Method for surmounting an obstacle by a robot vehicle

NASA Technical Reports Server (NTRS)

Wilcox, Brian H. (Inventor); Ohm, Timothy R. (Inventor)

1994-01-01

Surmounting obstacles in the path of a robot vehicle is accomplished by rotating the wheel forks of the vehicle about their transverse axes with respect to the vehicle body so as to shift most of the vehicle weight onto the rear wheels, and then driving the vehicle forward so as to drive the now lightly-loaded front wheels (only) over the obstacle. Then, after the front wheels have either surmounted or completely passed the obstacle (depending upon the length of the obstacle), the forks are again rotated about their transverse axes so as to shift most of the vehicle weight onto the front wheels. Then the vehicle is again driven forward so as to drive the now lightly-loaded rear wheels over the obstacle. Once the obstacle has been completely cleared and the vehicle is again on relatively level terrain, the forks are again rotated so as to uniformly distribute the vehicle weight between the front and rear wheels.

Comparison of storm response of streams in small, unmined and valley-filled watersheds, 1999-2001, Ballard fork, West Virginia

USGS Publications Warehouse

Messinger, Terence

2003-01-01

During storms when rainfall intensity exceeded about 1 inch per hour, peak unit runoff from the Unnamed Tributary (surface-mined and filled) Watershed exceeded peak unit runoff from the Spring Branch (unmined) Watershed in the Ballard Fork Watershed in southern West Virginia. During most storms, those with intensity less than about 1 inch per hour, peak unit (area-normalized) flows were greater from the Spring Branch Watershed than the Unnamed Tributary Watershed. One storm that produced less than an inch of rain before flow from the previous storm had receded caused peak unit flow from the Unnamed Tributary Watershed to exceed peak unit flow from the Spring Branch Watershed. Peak unit flow was usually similar in Spring Branch and Ballard Fork. Peak unit flows are expected to decrease with increasing watershed size in homogeneous watersheds; drainage area and proportion of the three watersheds covered by valley fills are 0.19 square mile (mi?) and 44 percent for the Unnamed Tributary Watershed, 0.53 mi? and 0 percent for the Spring Branch Watershed, and 2.12 mi? and 12 percent for the Ballard Fork Watershed. Following all storms with sufficient rainfall intensity, about 0.25 inches per hour, the storm hydrograph from the Unnamed Tributary Watershed showed a double peak, as a sharp initial rise was followed by a decrease in flow and then a delayed secondary peak of water that had apparently flowed through the valley fill. Hortonian (excess overland) flow may be important in the Unnamed Tributary Watershed during intense storms, and may cause the initial peak on the rising arm of storm hydrographs; the water composing the initial peaks may be conveyed by drainage structures on the mine. Ballard Fork and Spring Branch had hydrographs with single peaks, typical of elsewhere in West Virginia. During all storms with 1-hour rainfall greater than 0.75 inches or 24-hour rainfall greater than 1.75 inches during which all stream gages recorded a complete record, the Unnamed Tributary yielded the most total unit flow. In three selected major storms, total unit flow from the Unnamed Tributary during recessions exceeded storm flow, and its total unit flow was greatest among the streams during all three recessions. Runoff patterns from the mined watershed are influenced by the compaction of soils on the mine, the apparent low maximum rate of infiltration into the valley fill compared to that in the unmined, forested watershed, storage of water in the valley fill, and the absence of interception from trees and leaf litter. No storms during this study produced 1-hour or 24-hour rainfall in excess of the 5-year return period, and streamflow during this study never exceeded a magnitude equivalent to the 1.5-year return period; relative peak unit flow among the three streams in this study could be different in larger storms. Rainfall-runoff relations on altered landscapes are site-specific, and aspects of mining and reclamation practice that affect storm response may vary among mines.

Installation Restoration Program. Phase I: Records Search Minot Air Force Base, North Dakota.

DTIC Science & Technology

1984-12-01

MAFB and Grand Forks AFB. This move localized command along with •. •• insuring that assigned units would be capable of conducting aerial...were realigned !•;>•’ to become members of the 57th AD. Also on May 1, Grand Forks AFB was \\.•/ assigned to the 4th AD at F.E. Warren AFB, Wyoming...potential for vertical - - stratification of possible ground water contaminants. During drilling, Shelby tube samples should be taken to provide soils

4. Main Control Switchboard (south end rear), view to the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Main Control Switchboard (south end rear), view to the north, with item 2 (the load frequency control panel) visible in right foreground, through item 7 (generator Unit 4 control panel) obliquely visible on left side of the photograph. Part of item 1 (the synchronization monitor) is visible behind the phone on right side of photograph. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

Demonstration of Advanced Geophysics and Classification Methods on Munitions Response Sites - East Fork Valley Range Complex, Former Camp Hale

DTIC Science & Technology

2016-04-01

with cores of igneous and metamorphic rocks flanked by steeply dipping sedimentary rocks . The valley floors range in elevation from about 9,310 to...Camp Hale, East Fork Valley Range Complex Munitions Response Site. This project is one in a series of projects funded by ESTCP to use advanced...Technology Certification Program ft Feet FUDS Formerly Used Defense Site GPS Global Positioning System ID Identification IMU Inertial Measurement Unit

Photolithography and Selective Etching of an Array of Quartz Tuning Fork Resonators with Improved Impact Resistance Characteristics

NASA Astrophysics Data System (ADS)

Lee, Sungkyu

2001-08-01

Quartz tuning fork blanks with improved impact-resistant characteristics for use in Qualcomm mobile station modem (MSM)-3000 central processing unit (CPU) chips for code division multiple access (CDMA), personal communication system (PCS), and global system for mobile communication (GSM) systems were designed using finite element method (FEM) analysis and suitable processing conditions were determined for the reproducible precision etching of a Z-cut quartz wafer into an array of tuning forks. Negative photoresist photolithography for the additive process was used in preference to positive photoresist photolithography for the subtractive process to etch the array of quartz tuning forks. The tuning fork pattern was transferred via a conventional photolithographical chromium/quartz glass template using a standard single-sided aligner and subsequent negative photoresist development. A tightly adhering and pinhole-free 600/2000 Å chromium/gold mask was coated over the developed photoresist pattern which was subsequently stripped in acetone. This procedure was repeated on the back surface of the wafer. With the protective metallization area of the tuning fork geometry thus formed, etching through the quartz wafer was performed at 80°C in a ± 1.5°C controlled bath containing a concentrated solution of ammonium bifluoride to remove the unwanted areas of the quartz wafer. The quality of the quartz wafer surface finish after quartz etching depended primarily on the surface finish of the quartz wafer prior to etching and the quality of quartz crystals used. Selective etching of a 100 μm quartz wafer could be achieved within 90 min at 80°C. A selective etching procedure with reproducible precision has thus been established and enables the photolithographic mass production of miniature tuning fork resonators.

Advancing the Fork detector for quantitative spent nuclear fuel verification

DOE PAGES

Vaccaro, S.; Gauld, I. C.; Hu, J.; ...

2018-01-31

The Fork detector is widely used by the safeguards inspectorate of the European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) to verify spent nuclear fuel. Fork measurements are routinely performed for safeguards prior to dry storage cask loading. Additionally, spent fuel verification will be required at the facilities where encapsulation is performed for acceptance in the final repositories planned in Sweden and Finland. The use of the Fork detector as a quantitative instrument has not been prevalent due to the complexity of correlating the measured neutron and gamma ray signals with fuel inventories and operator declarations.more » A spent fuel data analysis module based on the ORIGEN burnup code was recently implemented to provide automated real-time analysis of Fork detector data. This module allows quantitative predictions of expected neutron count rates and gamma units as measured by the Fork detectors using safeguards declarations and available reactor operating data. This study describes field testing of the Fork data analysis module using data acquired from 339 assemblies measured during routine dry cask loading inspection campaigns in Europe. Assemblies include both uranium oxide and mixed-oxide fuel assemblies. More recent measurements of 50 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel are also analyzed. An evaluation of uncertainties in the Fork measurement data is performed to quantify the ability of the data analysis module to verify operator declarations and to develop quantitative go/no-go criteria for safeguards verification measurements during cask loading or encapsulation operations. The goal of this approach is to provide safeguards inspectors with reliable real-time data analysis tools to rapidly identify discrepancies in operator declarations and to detect potential partial defects in spent fuel assemblies with improved reliability and minimal false positive alarms. Finally, the results are summarized, and sources and magnitudes of uncertainties are identified, and the impact of analysis uncertainties on the ability to confirm operator declarations is quantified.« less

Advancing the Fork detector for quantitative spent nuclear fuel verification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vaccaro, S.; Gauld, I. C.; Hu, J.

The Fork detector is widely used by the safeguards inspectorate of the European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) to verify spent nuclear fuel. Fork measurements are routinely performed for safeguards prior to dry storage cask loading. Additionally, spent fuel verification will be required at the facilities where encapsulation is performed for acceptance in the final repositories planned in Sweden and Finland. The use of the Fork detector as a quantitative instrument has not been prevalent due to the complexity of correlating the measured neutron and gamma ray signals with fuel inventories and operator declarations.more » A spent fuel data analysis module based on the ORIGEN burnup code was recently implemented to provide automated real-time analysis of Fork detector data. This module allows quantitative predictions of expected neutron count rates and gamma units as measured by the Fork detectors using safeguards declarations and available reactor operating data. This study describes field testing of the Fork data analysis module using data acquired from 339 assemblies measured during routine dry cask loading inspection campaigns in Europe. Assemblies include both uranium oxide and mixed-oxide fuel assemblies. More recent measurements of 50 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel are also analyzed. An evaluation of uncertainties in the Fork measurement data is performed to quantify the ability of the data analysis module to verify operator declarations and to develop quantitative go/no-go criteria for safeguards verification measurements during cask loading or encapsulation operations. The goal of this approach is to provide safeguards inspectors with reliable real-time data analysis tools to rapidly identify discrepancies in operator declarations and to detect potential partial defects in spent fuel assemblies with improved reliability and minimal false positive alarms. Finally, the results are summarized, and sources and magnitudes of uncertainties are identified, and the impact of analysis uncertainties on the ability to confirm operator declarations is quantified.« less

Advancing the Fork detector for quantitative spent nuclear fuel verification

NASA Astrophysics Data System (ADS)

Vaccaro, S.; Gauld, I. C.; Hu, J.; De Baere, P.; Peterson, J.; Schwalbach, P.; Smejkal, A.; Tomanin, A.; Sjöland, A.; Tobin, S.; Wiarda, D.

2018-04-01

The Fork detector is widely used by the safeguards inspectorate of the European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) to verify spent nuclear fuel. Fork measurements are routinely performed for safeguards prior to dry storage cask loading. Additionally, spent fuel verification will be required at the facilities where encapsulation is performed for acceptance in the final repositories planned in Sweden and Finland. The use of the Fork detector as a quantitative instrument has not been prevalent due to the complexity of correlating the measured neutron and gamma ray signals with fuel inventories and operator declarations. A spent fuel data analysis module based on the ORIGEN burnup code was recently implemented to provide automated real-time analysis of Fork detector data. This module allows quantitative predictions of expected neutron count rates and gamma units as measured by the Fork detectors using safeguards declarations and available reactor operating data. This paper describes field testing of the Fork data analysis module using data acquired from 339 assemblies measured during routine dry cask loading inspection campaigns in Europe. Assemblies include both uranium oxide and mixed-oxide fuel assemblies. More recent measurements of 50 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel are also analyzed. An evaluation of uncertainties in the Fork measurement data is performed to quantify the ability of the data analysis module to verify operator declarations and to develop quantitative go/no-go criteria for safeguards verification measurements during cask loading or encapsulation operations. The goal of this approach is to provide safeguards inspectors with reliable real-time data analysis tools to rapidly identify discrepancies in operator declarations and to detect potential partial defects in spent fuel assemblies with improved reliability and minimal false positive alarms. The results are summarized, and sources and magnitudes of uncertainties are identified, and the impact of analysis uncertainties on the ability to confirm operator declarations is quantified.

The role of psychological determinants and demographic factors in consumer demand for farm-to-fork traceability systems.

PubMed

Myae, Aye Chan; Goddard, Ellen; Aubeeluck, Ashwina

2011-01-01

Traceability systems are an important tool (1) for tracking, monitoring, and managing product flows through the supply chain for better efficiency and profitability of suppliers, and (2) to improve consumer confidence in the face of serious food safety incidents. After the global bovine spongiform encephalopathy (BSE) crisis affected producers, consumers, trade, and the health status of animals and humans, new systems to help confirm the status of cattle products along the supply chain from farm to fork were implemented in many countries (Trautman et al. 2008 ). In this study, people's overall food safety beliefs are explored with the main objective of measuring the link between their food safety beliefs and their attitudes toward traceability. A comparison is made among English-speaking Canadians, French-speaking Canadians, and Japanese consumers. In the study, an Internet-based survey was used to collect data from nationally representative samples of the population in Canada-English (1275), Canada-French (343), and Japanese (1940) in the summer of 2009. Respondents' interests in traceability systems are clearly linked to their sense that the industry is primarily responsible for any food safety outbreaks. Moreover, it is clear that certain segments of the population in all samples feel strongly about the importance of farm to fork traceability in beef; thus, policymakers may wish to consider extending traceability beyond the point of slaughter as a way of encouraging beef sales in Canada.

Potential effects of climate change on streamflow, eastern and western slopes of the Sierra Nevada, California and Nevada

USGS Publications Warehouse

Jeton, A.E.; Dettinger, M.D.; Smith, J. LaRue

1996-01-01

Precipitation-runoff models of the East Fork Carson and North Fork American Rivers were developed and calibrated for use in evaluating the sensitivity of streamflow in the north-central Sierra Nevada to climate change. The East Fork Carson River drains part of the rain-shadowed, eastern slope of the Sierra Nevada and is generally higher than the North Fork American River, which drains the wetter, western slope. First, a geographic information system was developed to describe the spatial variability of basin characteristics and to help estimate model parameters. The result was a partitioning of each basin into noncontiguous, but hydrologically uniform, land units. Hydrologic descriptions of these units were developed and the Precipitation- Runoff Modeling System (PRMS) was used to simulate water and energy balances for each unit in response to daily weather conditions. The models were calibrated and verified using historical streamflows over 22-year (Carson River) and 42-year (American River) periods. Simulated annual streamflow errors average plus 10 percent of the observed flow for the East Fork Carson River basin and plus 15 percent for the North Fork American River basin. Interannual variability is well simulated overall, but, at daily scales, wet periods are simulated more accurately than drier periods. The simulated water budgets for the two basins are significantly different in seasonality of streamflow, sublimation, evapotranspiration, and snowmelt. The simulations indicate that differences in snowpack and snowmelt timing can play pervasive roles in determining the sensitivity of water resources to climate change, in terms of both resource availability and amount. The calibrated models were driven by more than 25 hypothetical climate-change scenarios, each 100 years long. The scenarios were synthesized and spatially disaggregated by methods designed to preserve realistic daily, monthly, annual, and spatial statistics. Simulated streamflow timing was not very sensitive to changes in mean precipitation, but was sensitive to changes in mean temperatures. Changes in annual streamflow amounts were amplified reflections of imposed mean precipitation changes, with especially large responses to wetter climates. In contrast, streamflow amount was surprisingly insensitive to mean temperature changes as a result of temporal links between peak snowmelt and the beginning of warm-season evapotranspiration. Comparisons of simulations driven by temporally detailed climate-model changes in which mean temperature changes vary from month to month and simulations in which uniform climate changes were imposed throughout the year indicate that the snowpack accumulates the influences of short-term conditions so that season average climate changes were more important than shorter term changes.

North Fork Caspar Creek stream biology study

Treesearch

Micheal S. Parker

1991-01-01

Timber harvesting is one of the most widespread land-uses in forested watersheds throughout western North America. It has long been recognized that timber removal, primarily through clearcutting, may have significant impacts on some environmental conditions within streams draining forested watersheds. It is also clear that logging related impacts are highly variable...

14. Station Control Batteries and Battery Chargers, view to the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. Station Control Batteries and Battery Chargers, view to the northeast. The original battery charger is the center cabinet on the left side of photograph, with the new charger on the far left of photograph and a circuit breaker unit for the chargers is visible in the center of the photograph. The batteries are visible on three racks through the open doorway. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

17. Governor Accumulator Tank Compressor and motor located along rear ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. Governor Accumulator Tank Compressor and motor located along rear corridor between Units 3 and 4, view to the west. The compressor motor is located just right of center in photograph. The pressure tank on the right side of the photograph is a reserve pressure tank for governor system. The pressure tank on the left side of the photograph is the original instrument air pressure tank. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

Initial Geomorphic Responses to Removal of Milltown Dam, Clark Fork River, Montana, USA

NASA Astrophysics Data System (ADS)

Wilcox, A. C.; Brinkerhoff, D.; Woelfle-Erskine, C.

2008-12-01

The removal of Milltown Dam on the Clark Fork River, Montana, USA, is creating a field-scale experiment on upstream and downstream responses to dam removal and on how gravel-bed rivers respond to sediment pulses. Milltown Dam was removed in 2008, reconnecting the Clark Fork River to its upstream basin in terms of sediment transport and fish passage. This dam removal is especially notable because (1) it is the largest dam removal to date in the United States in terms of the volume of reservoir sediment potentially available for downstream transport (over 3 million m3; 1.7 million m3 are being mechanically removed); and (2) the dam is the downstream end of the largest Superfund site in the United States, the Clark Fork Complex, and reservoir sediments are composed largely of contaminated mine tailings. Data collection on pre- and post-dam removal channel morphology, bed sediment characteristics, and sediment loads are being used to investigate spatial and temporal patterns of sediment transport and deposition associated with this dam removal. In the first several months following breaching of the dam, snowmelt runoff with a 3-year recurrence interval peak caused substantial erosion and downstream transport of metals-laden sediments from Milltown reservoir. Reservoir sediments in the Clark Fork arm of Milltown reservoir eroded at levels far exceeding modeling predictions as a result of both incision to the new base level created by dam removal and bank retreat of over 200 m in reaches upstream of a constructed bypass reach and remediation area. Copper and other metals in these eroded reservoir sediments provide a tracer for identifying whether sediment deposits observed downstream of the dam originated from Milltown reservoir or uncontaminated tributaries and indicate that Milltown sediments have reached over 200 km downstream. Downstream deposition has been greatest along channel margins and in side-channel areas, whereas the transport capacity of the active channel has limited channel changes there.

Spillway for Benbrook Dam, Clear Fork of the Trinity River, Texas: Model Investigation

DTIC Science & Technology

1949-03-01

section at tho too of tho spillway to tho trapezoidal cross section of tho exit channel. Concreto training walls 11 ft in height will confine the...embankment sections to the spillway, thus eliminating the concreto sec- tiona . This procedure would require training walls at each abutment t o protect

Metals Release From Mining-Impacted Streambed Sediments In The North Fork Of Clear Creek, Colorado

EPA Science Inventory

Many miles of streams in the US (and worldwide) are contaminated by metals originating from both active and abandoned mine sites. Streams affected by mine drainage are often toxic to aquatic life. Thus, it is desirable to remediate these sites through removal or treatment of th...

Trends in streamflow and suspended sediment after logging, North Fork Caspar Creek

Treesearch

Jack Lewis; Elizabeth T. Keppeler

2007-01-01

Streamflow and suspended sediment were intensively monitored at fourteen gaging stations before and after logging a second-growth redwood (Sequoia sempervirens) forest. About 50 percent of the watershed was harvested, primarily by clear-cutting with skyline-cable systems. New road construction and tractor skidding were restricted to gently-sloping...

16 CFR 1512.6 - Requirements for steering system.

Code of Federal Regulations, 2014 CFR

2014-01-01

... stem insertion mark. Quill-type handlebar stems shall contain a permanent ring or mark which clearly indicates the minimum insertion depth of the handlebar stem into the fork assembly. The insertion mark shall not affect the structural integrity of the stem and shall not be less than 21/2 times the stem...

16 CFR 1512.6 - Requirements for steering system.

Code of Federal Regulations, 2012 CFR

2012-01-01

... stem insertion mark. Quill-type handlebar stems shall contain a permanent ring or mark which clearly indicates the minimum insertion depth of the handlebar stem into the fork assembly. The insertion mark shall not affect the structural integrity of the stem and shall not be less than 21/2 times the stem...

16 CFR 1512.6 - Requirements for steering system.

Code of Federal Regulations, 2010 CFR

2010-01-01

... stem insertion mark. The handlebar stem shall contain a permanent ring or mark which clearly indicates the minimum insertion depth of the handlebar stem into the fork assembly. The insertion mark shall not affect the structural integrity of the stem and shall not be less than 21/2 times the stem diameter from...

16 CFR 1512.6 - Requirements for steering system.

Code of Federal Regulations, 2011 CFR

2011-01-01

... stem insertion mark. The handlebar stem shall contain a permanent ring or mark which clearly indicates the minimum insertion depth of the handlebar stem into the fork assembly. The insertion mark shall not affect the structural integrity of the stem and shall not be less than 21/2 times the stem diameter from...

16 CFR § 1512.6 - Requirements for steering system.

Code of Federal Regulations, 2013 CFR

2013-01-01

... stem insertion mark. Quill-type handlebar stems shall contain a permanent ring or mark which clearly indicates the minimum insertion depth of the handlebar stem into the fork assembly. The insertion mark shall not affect the structural integrity of the stem and shall not be less than 21/2 times the stem...

Environmental settings of the South Fork Iowa River basin, Iowa, and the Bogue Phalia basin, Mississippi, 2006-10

USGS Publications Warehouse

McCarthy, Kathleen A.; Rose, Claire E.; Kalkhoff, Stephen J.

2012-01-01

Studies of the transport and fate of agricultural chemicals in different environmental settings were conducted by the U.S. Geological Survey (USGS) National Water-Quality Assessment (NAWQA) Program's Agricultural Chemicals Team (ACT) at seven sites across the Nation, including the South Fork Iowa River basin in central Iowa and the Bogue Phalia basin in northwestern Mississippi. The South Fork Iowa River basin is representative of midwestern agriculture, where corn and soybeans are the predominant crops and a large percentage of the cultivated land is underlain by artificial drainage. The Bogue Phalia basin is representative of corn, soybean, cotton, and rice cropping in the humid, subtropical southeastern United States. Details of the environmental settings of these basins and the data-collection activities conducted by the USGS ACT over the 2006-10 study period are described in this report.

Environmental covariates associated with Cambarus veteranus (Decapoda: Cambaridae), an imperiled Appalachian crayfish endemic to West Virginia, USA

USGS Publications Warehouse

Loughman, Zachary J.; Welsh, Stuart A.; Sadecky, Nicole M.; Dillard, Zachary W.; Scott, R. Katie

2016-01-01

Cambarus veteranus Faxon, 1914, a narrow endemic crayfish native to the Upper Guyandotte River Basin (UGB) in West Virginia, USA, was petitioned in 2014 by the United States Fish and Wildlife Service to be listed as endangered, but a status survey was recommended to determine if listing was warranted. During May and June 2015, surveys were undertaken across the UGB to determine the current distribution of the species. A total of 71 sites were sampled, including all streams where the species was previously recorded, as well as semi-randomly selected streams, with 1-9 125 m long sites sampled per wadeable stream. Physiochemical and physical habitat data (based on the Qualitative Habitat Evaluation Index, QHEI) were obtained at each site to determine abiotic factors that were associated with the presence of C. veteranus. Site detection or non-detection of C. veteranus and associated site covariates were modeled using logistic regression to determine covariates associated with the presence of the species. Cambarus veteranus was present in both the Pinnacle Creek and Clear Fork/Laurel Fork watersheds at 10 sites, but it was not observed in the remaining 61 sites. An additive effects model with conductivity and QHEI was selected as the best approximating model. Cambarusveteranus was associated with lower than average UGB conductivity (379 µS) and high (>80) QHEI score. All sites where C. veteranus was not detected had higher conductivity and/or lower QHEI scores.

Food safety and organic meats.

PubMed

Van Loo, Ellen J; Alali, Walid; Ricke, Steven C

2012-01-01

The organic meat industry in the United States has grown substantially in the past decade in response to consumer demand for nonconventionally produced products. Consumers are often not aware that the United States Department of Agriculture (USDA) organic standards are based only on the methods used for production and processing of the product and not on the product's safety. Food safety hazards associated with organic meats remain unclear because of the limited research conducted to determine the safety of organic meat from farm-to-fork. The objective of this review is to provide an overview of the published results on the microbiological safety of organic meats. In addition, antimicrobial resistance of microbes in organic food animal production is addressed. Determining the food safety risks associated with organic meat production requires systematic longitudinal studies that quantify the risks of microbial and nonmicrobial hazards from farm-to-fork.

Maps showing anomaly patterns for silver, molybdenum, lead, and zinc in altered rocks and soils, Williams Fork and St. Louis Peak Roadless Areas, Clear Creek, Grand, and Summit counties, Colorado

USGS Publications Warehouse

Eppinger, R.G.; Theobald, P.K.; Barton, H.N.; Carlson, R.R.

1985-01-01

The U.S. Geological Survey and the U.S Bureau of Mines conducted field studies from 1979 through 1982 to assess the mineral resource potential of the Williams Fork and St. Louis Peak Roadless Areas and the Vasquez Peak Wilderness Study Area.  Included were geological, geochemical, and geophysical studies by the U.S. Geological Survey and investigation of known prospects and mines by the U.S. Bureau of Mines.  The area of study is located in the Arapaho National Forest, nort of Interstate Highway 70, west of U.S. Highway 40, and east of the Blue River, on the west side of the Front Range (fig. 1).

Recovery of thermophilic Campylobacter by three sampling methods from classified river sites in Northeast Georgia, USA

USDA-ARS?s Scientific Manuscript database

It is not clear how best to sample streams for the detection of Campylobacter which may be introduced from agricultural or community land use. Fifteen sites in the watershed of the South Fork of the Broad River (SFBR) in Northeastern Georgia, USA, were sampled in three seasons. Seven sites were cl...

Observed And Modeled Seasonal Trends In Dissolved And Particulate Cu, Fe, Mn, And Zn In A Mining-Impacted Stream

EPA Science Inventory

North Fork Clear Creek (NFCC) in Colorado, an acid-mine drainage (AMD) impacted stream, was chosen to examine the distribution of dissolved and particulate Cu, Fe, Mn, and Zn in the water column, with respect to seasonal hydrologic controls. NFCC is a high-gradient stream with d...

Replication fork reversal triggers fork degradation in BRCA2-defective cells.

PubMed

Mijic, Sofija; Zellweger, Ralph; Chappidi, Nagaraja; Berti, Matteo; Jacobs, Kurt; Mutreja, Karun; Ursich, Sebastian; Ray Chaudhuri, Arnab; Nussenzweig, Andre; Janscak, Pavel; Lopes, Massimo

2017-10-16

Besides its role in homologous recombination, the tumor suppressor BRCA2 protects stalled replication forks from nucleolytic degradation. Defective fork stability contributes to chemotherapeutic sensitivity of BRCA2-defective tumors by yet-elusive mechanisms. Using DNA fiber spreading and direct visualization of replication intermediates, we report that reversed replication forks are entry points for fork degradation in BRCA2-defective cells. Besides MRE11 and PTIP, we show that RAD52 promotes stalled fork degradation and chromosomal breakage in BRCA2-defective cells. Inactivation of these factors restores reversed fork frequency and chromosome integrity in BRCA2-defective cells. Conversely, impairing fork reversal prevents fork degradation, but increases chromosomal breakage, uncoupling fork protection, and chromosome stability. We propose that BRCA2 is dispensable for RAD51-mediated fork reversal, but assembles stable RAD51 nucleofilaments on regressed arms, to protect them from degradation. Our data uncover the physiopathological relevance of fork reversal and illuminate a complex interplay of homologous recombination factors in fork remodeling and stability.BRCA2 is involved in both homologous recombination (HR) and the protection of stalled replication forks from degradation. Here the authors reveal how HR factors cooperate in fork remodeling, showing that BRCA2 supports RAD51 loading on the regressed arms of reversed replication forks to protect them from degradation.

The UL5 and UL52 subunits of the herpes simplex virus type 1 helicase-primase subcomplex exhibit a complex interdependence for DNA binding.

PubMed

Biswas, N; Weller, S K

2001-05-18

Herpes simplex virus type 1 encodes a heterotrimeric helicase-primase complex composed of the products of the UL5, UL52, and UL8 genes. The UL5 protein contains seven motifs found in all members of helicase Superfamily 1 (SF1), and the UL52 protein contains several conserved motifs found in primases; however, the contributions of each subunit to the biochemical activities of the subcomplex are not clear. In this work, the DNA binding properties of wild type and mutant subcomplexes were examined using single-stranded, duplex, and forked substrates. A gel mobility shift assay indicated that the UL5-UL52 subcomplex binds more efficiently to the forked substrate than to either single strand or duplex DNA. Although nucleotides are not absolutely required for DNA binding, ADP stimulated the binding of UL5-UL52 to single strand DNA whereas ATP, ADP, and adenosine 5'-O-(thiotriphosphate) stimulated the binding to a forked substrate. We have previously shown that both subunits contact single-stranded DNA in a photocross-linking assay (Biswas, N., and Weller, S. K. (1999) J. Biol. Chem. 274, 8068-8076). In this study, photocross-linking assays with forked substrates indicate that the UL5 and UL52 subunits contact the forked substrates at different positions, UL52 at the single-stranded DNA tail and UL5 near the junction between single-stranded and double-stranded DNA. Neither subunit was able to cross-link a forked substrate when 5-iododeoxyuridine was located within the duplex portion. Photocross-linking experiments with subcomplexes containing mutant versions of UL5 and wild type UL52 indicated that the integrity of the ATP binding region is important for DNA binding of both subunits. These results support our previous proposal that UL5 and UL52 exhibit a complex interdependence for DNA binding (Biswas, N., and Weller, S. K. (1999) J. Biol. Chem. 274, 8068-8076) and indicate that the UL52 subunit may play a more active role in helicase activity than had previously been thought.

75 FR 25197 - Shasta Trinity National Forest, South Fork Management Unit, California Salt Timber Harvest and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-07

... use telecommunication devices for the deaf (TDD) may call the Federal Information Relay Service (FIRS..., Environmental Protection Information Center and Kiamath Forest Alliance. On February 5, 2010, Appeal Reviewing...

77 FR 50668 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-22

... County, Texas, and Incorporated Areas. Specifically, it addresses the flooding sources: Black Fork Creek, Black Fork Creek Tributary BF-1, Black Fork Creek Tributary BF-M-1, Black Fork Creek Tributary D, Black Fork Creek Tributary D-1, Black Fork Creek Tributary D-2, Black Fork Creek Tributary D-3, Butler Creek...

Effects of forest harvesting on large organic debris in coastal streams

Treesearch

Christopher G. Surfleet; Robert R. Ziemer

1996-01-01

Abstract - Large organic debris (LOD) was inventoried in two coastal streams to assess the impacts of forest harvesting on LOD recruitment in 90-year-old, second-growth redwood and fir stands on the Jackson Demonstration State Forest in northern California. One stream, North Fork of Caspar Creek, drained a 508-ha watershed that had been 60% clear-cut, with riparian...

3. View of Clark Fork Vehicle Bridge facing southwest. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. View of Clark Fork Vehicle Bridge facing southwest. Bridge from north shore of Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

An allocation of undiscovered oil and gas resources to Big South Fork National Recreation Area and Obed Wild and Scenic River, Kentucky and Tennessee

USGS Publications Warehouse

Schenk, Christopher J.; Klett, Timothy R.; Charpentier, Ronald R.; Cook, Troy A.; Pollastro, Richard M.

2006-01-01

The U.S. Geological Survey (USGS) estimated volumes of undiscovered oil and gas resources that may underlie Big South Fork National Recreation Area and Obed Wild and Scenic River in Kentucky and Tennessee. Applying the results of existing assessments of undiscovered resources from three assessment units in the Appalachian Basin Province and three plays in the Cincinnati Arch Province that include these land parcels, the USGS allocated approximately (1) 16 billion cubic feet of gas, 15 thousand barrels of oil, and 232 thousand barrels of natural gas liquids to Big South Fork National Recreation Area; and (2) 0.5 billion cubic feet of gas, 0.6 thousand barrels of oil, and 10 thousand barrels of natural gas liquids to Obed Wild and Scenic River. These estimated volumes of undiscovered resources represent potential volumes in new undiscovered fields, but do not include potential additions to reserves within existing fields.

4. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shoreof Clark Fork River showing 4 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Patterns of Storm Injury and Tree Response

Treesearch

Kevin Smith; Walter Shortle; Kenneth Dudzik

2001-01-01

The ice storm of January 1998 in the northeastern United States and adjacent Canada was an extreme example of severe weather that injures trees every year. Broken branches, split branch forks, and snapped stems are all examples of storm injury.

2. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

7. View of Clark Fork Vehicle Bridge facing northwest. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. View of Clark Fork Vehicle Bridge facing northwest. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

1. View of Clark Fork Vehicle Bridge facing west. Panorama ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. View of Clark Fork Vehicle Bridge facing west. Panorama showing the entire span of bridge from north shore of the Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Profile surveys along Henrys Fork, Idaho, and Logan River and Blacksmith Fork, Utah

USGS Publications Warehouse

Herron, William Harrison

1916-01-01

In order to determine the location of undeveloped water powers the United States Geological Survey has from time to time, alone and in cooperation with State organizations, made surveys and profiles of some of the rivers of the United States that are adapted to the development of power by low or medium heads of 20 to 100 feet.The surveys are made by means of plane table and stadia. Elevations are based on heights derived from primary or precise levels of the United States Geological Survey. The maps/are made in the field, and show not only the outlines of the river banks, the islands, the positions of rapids/falls, shoals, and existing dams, and the crossings of all ferries and roads, but the contours of banks to an elevation high enough to indicate the possibility of using the stream. The elevations of the various bench marks left are noted on the field sheets in their proper positions. The figures given with the gaging stations shown on the maps indicate the elevation of the zero of the gage.

5. View of Clark Fork Vehicle Bridge facing east. Bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. View of Clark Fork Vehicle Bridge facing east. Bridge from south shore of Clark Fork River-southernmost span. 1900-era Northern Pacific Railway Bridge in background. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Phytotoxicity of floodplain soils contaminated with trace metals along the clark fork river, Grant-Kohrs Ranch National Historic Site, Deer Lodge, Montana, United States

USGS Publications Warehouse

Rader, B.R.; Nimmo, D.W.R.; Chapman, P.L.

1997-01-01

Concentrations of metals in sediments and soils deposited along the floodplain of the Clark Fork River, within the Grant-Kohrs Ranch National Historic Site, Deer Lodge, Montana, USA, have exceeded maximum background concentrations in the United States for most metals tested. As a result of mining and smelting activities, portions of the Deer Lodge Valley, including the Grant-Kohrs Ranch, have received National Priority List Designation under the Comprehensive Environmental Response, Compensation and Liability Act. Using a series of plant germination tests, pH measurements, and metal analyses, this study investigated the toxicity of soils from floodplain 'slicken' areas, bare spots devoid of vegetation, along the Clark Fork River. The slicken soils collected from the Grant-Kohrs Ranch were toxic to all four plant species tested. The most sensitive endpoint in the germination tests was root length and the least sensitive was emergence. Considering emergence, the most sensitive species was the resident grass species Agrostis gigantea. The sensitivities were reversed when root lengths were examined, with Echinochloa crusgalli showing the greatest sensitivity. Both elevated concentrations of metals and low pH were necessary to produce an acutely phytotoxic response in laboratory seed germination tests using slicken soils. Moreover, pH values on the Grant-Kohrs Ranch appear to be a better predictor of acutely phytotoxic conditions than total metal levels.

50 CFR 226.212 - Critical habitat for 13 Evolutionarily Significant Units (ESUs) of salmon and steelhead...

Code of Federal Regulations, 2014 CFR

2014-10-01

... floodplain connectivity to form and maintain physical habitat conditions and support juvenile growth and... endpoint(s) in: Crooked Creek (46.3033, -123.6222); East Fork Grays River (46.4425, -123.4081); Fossil...

50 CFR 226.212 - Critical habitat for 13 Evolutionarily Significant Units (ESUs) of salmon and steelhead...

Code of Federal Regulations, 2011 CFR

2011-10-01

... floodplain connectivity to form and maintain physical habitat conditions and support juvenile growth and... endpoint(s) in: Crooked Creek (46.3033, -123.6222); East Fork Grays River (46.4425, -123.4081); Fossil...

50 CFR 226.212 - Critical habitat for 13 Evolutionarily Significant Units (ESUs) of salmon and steelhead...

Code of Federal Regulations, 2013 CFR

2013-10-01

... floodplain connectivity to form and maintain physical habitat conditions and support juvenile growth and... endpoint(s) in: Crooked Creek (46.3033, -123.6222); East Fork Grays River (46.4425, -123.4081); Fossil...

50 CFR 226.212 - Critical habitat for 13 Evolutionarily Significant Units (ESUs) of salmon and steelhead...

Code of Federal Regulations, 2012 CFR

2012-10-01

... floodplain connectivity to form and maintain physical habitat conditions and support juvenile growth and... endpoint(s) in: Crooked Creek (46.3033, -123.6222); East Fork Grays River (46.4425, -123.4081); Fossil...

50 CFR 226.212 - Critical habitat for 13 Evolutionarily Significant Units (ESUs) of salmon and steelhead...

Code of Federal Regulations, 2010 CFR

2010-10-01

... floodplain connectivity to form and maintain physical habitat conditions and support juvenile growth and... endpoint(s) in: Crooked Creek (46.3033, -123.6222); East Fork Grays River (46.4425, -123.4081); Fossil...

Comparative geomorphic analysis of surficial deposits at three central Appalachian watersheds: Implications for controls on sediment-transport efficiency

NASA Astrophysics Data System (ADS)

Taylor, Stephen B.; Steven Kite, J.

2006-08-01

Factors that control the routing and storage of sediments in the Appalachian region are poorly understood. This study involves a comparative geomorphic analysis of three watersheds underlain by sandstones and shales of the Acadian clastic wedge. These areas include the Fernow Experimental Forest, Tucker County, West Virginia; the North Fork basin, Pocahontas County, West Virginia; and the Little River basin, Augusta County, Virginia. GIS-based analyses of surficial map units allow first-order approximation of sediment-storage volumes in valley bottoms. Estimates of volumes are examined in tandem with morphometric analyses and the distribution of bedrock channels to make inferences regarding controls on sediment-transport efficiency in the central Appalachians. The Fernow and North Fork areas are characterized by V-shaped valleys with mixed reaches of alluvial-bedrock channels distributed throughout the drainage network. In contrast, the Little River valley is notably wider and gravelly alluvial fill is abundant. Comparator watershed parameters for the Fernow, North Fork and Little River areas include, respectively: (1) basin area = 15.2 km 2, 49.3 km 2, 41.5 km 2; (2) basin relief = 0.586 km, 0.533 km, 0.828 km; (3) drainage density = 4.2 km - 1 , 3.3 km - 1 , 4.7 km - 1 ; (4) ruggedness = 2.5, 1.7, 3.9; (5) Shreve magnitude = 139, 287, 380; (6) total valley-bottom area (km 2) = 0.76 km 2, 1.86 km 2, 3.09 km 2; (7) average hillslope gradients = 17.2°, 18.4°, 22.1°; (8) total debris-fan surface area = 0.113 km 2, 0.165 km 2, 0.486 km 2; and (9) debris-fan frequency = 2.0 km - 2 , 1.0 km - 2 , 2.8 km - 2 . The storage volumes in valley bottoms were estimated using map polygon areas and surface heights above channel grade. The Little River contains significantly higher sediment volumes in floodplain, terrace and fan storage compartments; total volumes of the valley bottoms are approximately twice that of the Fernow and North Fork areas combined. Unit storage volumes for the Fernow, North Fork and Little River are 5.2 × 10 4 m 3 km - 2 , 5.5 × 10 4 m 3 km - 2 and 1.6 × 10 5 m 3 km - 2 , respectively. A conceptual model postulates that valley-width morphometry and style of delivery from hillslopes are the primary factors controlling the efficiency of sediment transport. Steep, debris-flow-prone hillslopes at the Little River deliver high volumes of gravelly sediment at magnitudes greater than transport capacity of the channel. Patterns of stream power are complex, as low-order tributaries are under capacity and high-order tributaries over capacity with respect to sediment load. Aggraded alluvial fill insulates valley-floor bedrock from vertical erosion and valley widening dominates. Expansion of the valley width creates a positive response via increased storage capacity and lower unit stream power. Conversely, the Fernow and North Fork are characterized by diffusive mass movement on hillslopes with incremental bedload transport to higher-order tributaries. Rates of hillslope delivery are balanced by the rate of channel export. Mixed alluvial-bedrock reaches provide the optimal channel configuration for active incision of the valley floor. Low expansion of valley width promotes high unit stream power and processes of vertical erosion. The model implies that the Fernow and North Fork have been more effective at sediment transport during the Late Quaternary. Given similar climatic and tectonic settings, variation in bedrock lithofacies is likely the primary factor modulating the efficiency of sediment transport.

8. View of Clark Fork Vehicle Bridge facing southwest. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. View of Clark Fork Vehicle Bridge facing southwest. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

20. View of Clark Fork Vehicle Bridge facing up. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. View of Clark Fork Vehicle Bridge facing up. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

18. View of Clark Fork Vehicle Bridge facing north. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

18. View of Clark Fork Vehicle Bridge facing north. Looking at north concrete abutment and timber stringers. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

USDA-ARS update

USDA-ARS?s Scientific Manuscript database

Potato research at the Red River Valley Agricultural Research Center is conducted by the Sugarbeet & Potato Research Unit at two locations: the Northern Crop Science Laboratory in Fargo, ND and the Potato Research Worksite located in East Grand Forks, MN. Research in Fargo is laboratory oriented an...

19. View of Clark Fork Vehicle Bridge facing north. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. View of Clark Fork Vehicle Bridge facing north. Looking at north abutment and underside of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

22. View of Clark Fork Vehicle Bridge facing downwest side. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. View of Clark Fork Vehicle Bridge facing down-west side. Looking at road deck and vertical laced channel. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

21. View of Clark Fork Vehicle Bridge facing west. Looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

21. View of Clark Fork Vehicle Bridge facing west. Looking at bridge deck, guard rail, juncture of two bridge spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost span. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Reconnaissance Geologic Map of the Hayfork 15' Quadrangle, Trinity County, California

USGS Publications Warehouse

Irwin, William P.

2010-01-01

The Hayfork 15' quadrangle is located just west of the Weaverville 15' quadrangle in the southern part of the Klamath Mountains geologic province of northern California. It spans parts of six generally north-northwest-trending tectonostratigraphic terranes that are, from east to west, the Eastern Klamath, Central Metamorphic, North Fork, Eastern Hayfork, Western Hayfork, and Rattlesnake Creek terranes. Remnants of a once-widespread postaccretionary overlap assemblage, the Cretaceous Great Valley sequence, crop out at three localities in the southern part of the Hayfork quadrangle. The Tertiary fluvial and lacustrine Weaverville Formation occupies a large, shallow, east-northeast-trending graben in the south half of the quadrangle. The small area of Eastern Klamath terrane is part of the Oregon Mountain outlier, which is more widely exposed to the east in the Weaverville 15' quadrangle. It was originally mapped as a thrust plate of Bragdon(?) Formation, but it is now thought by some to be part of an outlier of Yreka terrane that has been dislocated 60 km southward by the La Grange Fault. The Central Metamorphic terrane, which forms the footwall of the La Grange Fault, was formed by the eastward subduction of oceanic crustal basalt (the Salmon Hornblende Schist) and its overlying siliceous sediments with interbedded limestone (the Abrams Mica Schist) beneath the Eastern Klamath terrane. Rb-Sr analysis of the Abrams Mica Schist indicates a Middle Devonian metamorphic age of approximately 380 Ma, which probably represents the age of subduction. The North Fork terrane, which is faulted against the western boundary of the Central Metamorphic terrane, consists of the Permian(?) North Fork ophiolite and overlying broken formation and melange of Permian to Early Jurassic (Pliensbachian) marine metasedimentary and metavolcanic rocks. The ophiolite, which crops out along the western border of the terrane, is thrust westward over the Eastern Hayfork terrane. The Eastern Hayfork terrane is a broken formation and melange of volcanic and sedimentary rocks, including chert and blocks of amphibolite, limestone, and serpentinized ultramafic rocks. The chert contains radiolarians of Permian and Triassic ages, but none of clearly Jurassic age. In contrast, the cherts of the North Fork terrane contain Early and Middle Jurassic radiolarians in addition to those of Permian and Triassic ages; also, some limestones of the Eastern Hayfork terrane contain fossil faunas of Tethyan affinity, but those of the North Fork terrane do not. The Western Hayfork terrane is an andesitic volcanic arc that was accreted to the Eastern Hayfork terrane. It consists mainly of metavolcaniclastic andesitic agglomerate and tuff, as well as argillite and chert, and it includes the dioritic Ironside Mountain batholith, which intruded during Middle Jurassic time. Two large patches of Western Hayfork terrane mapped in the central part of the Eastern Hayfork terrane may be exposed through windows in the Eastern Hayfork terrane, although the structural relation is not clear. The Rattlesnake Creek terrane is a melange that occupies only a small area in the southwest corner of the Hayfork quadrangle; however, it is a major unit in the Hyampom 15' quadrangle to the west. It consists mainly of broken and sheared ophiolitic rocks of probable Permian or early Mesozoic age. The Cretaceous Great Valley sequence overlap assemblage here postdates the Early Cretaceous (approximately 136 Ma) emplacement of the Shasta Bally batholith, which is widely exposed to the east in the Weaverville 15' quadrangle. The Great Valley sequence once covered much of the southern Klamath Mountains; however, in the Hayfork quadrangle, only three small patches remain near its southern boundary. Weakly consolidated nonmarine sedimentary rocks of the Weaverville Formation of mid-Tertiary age, which contain abundant fossil plants, occupy a large, shallow, east-northeast-trending graben

29 CFR 1910.178 - Powered industrial trucks.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., design, maintenance, and use of fork trucks, tractors, platform lift trucks, motorized hand trucks, and... new powered industrial trucks acquired and used by an employer shall meet the design and construction... that the electrical fittings and equipment are so designed, constructed and assembled that the units...

29 CFR 1910.178 - Powered industrial trucks.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., design, maintenance, and use of fork trucks, tractors, platform lift trucks, motorized hand trucks, and... new powered industrial trucks acquired and used by an employer shall meet the design and construction... that the electrical fittings and equipment are so designed, constructed and assembled that the units...

29 CFR 1910.178 - Powered industrial trucks.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., design, maintenance, and use of fork trucks, tractors, platform lift trucks, motorized hand trucks, and... new powered industrial trucks acquired and used by an employer shall meet the design and construction... that the electrical fittings and equipment are so designed, constructed and assembled that the units...

29 CFR 1910.178 - Powered industrial trucks.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., design, maintenance, and use of fork trucks, tractors, platform lift trucks, motorized hand trucks, and... new powered industrial trucks acquired and used by an employer shall meet the design and construction... that the electrical fittings and equipment are so designed, constructed and assembled that the units...

29 CFR 1910.178 - Powered industrial trucks.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., design, maintenance, and use of fork trucks, tractors, platform lift trucks, motorized hand trucks, and... new powered industrial trucks acquired and used by an employer shall meet the design and construction... that the electrical fittings and equipment are so designed, constructed and assembled that the units...

Rad53 regulates replication fork restart after DNA damage in Saccharomyces cerevisiae

PubMed Central

Szyjka, Shawn J.; Aparicio, Jennifer G.; Viggiani, Christopher J.; Knott, Simon; Xu, Weihong; Tavaré, Simon; Aparicio, Oscar M.

2008-01-01

Replication fork stalling at a DNA lesion generates a damage signal that activates the Rad53 kinase, which plays a vital role in survival by stabilizing stalled replication forks. However, evidence that Rad53 directly modulates the activity of replication forks has been lacking, and the nature of fork stabilization has remained unclear. Recently, cells lacking the Psy2–Pph3 phosphatase were shown to be defective in dephosphorylation of Rad53 as well as replication fork restart after DNA damage, suggesting a mechanistic link between Rad53 deactivation and fork restart. To test this possibility we examined the progression of replication forks in methyl-methanesulfonate (MMS)-damaged cells, under different conditions of Rad53 activity. Hyperactivity of Rad53 in pph3Δ cells slows fork progression in MMS, whereas deactivation of Rad53, through expression of dominant-negative Rad53-KD, is sufficient to allow fork restart during recovery. Furthermore, combined deletion of PPH3 and PTC2, a second, unrelated Rad53 phosphatase, results in complete replication fork arrest and lethality in MMS, demonstrating that Rad53 deactivation is a key mechanism controlling fork restart. We propose a model for regulation of replication fork progression through damaged DNA involving a cycle of Rad53 activation and deactivation that coordinates replication restart with DNA repair. PMID:18628397

23. View of Clark Fork Vehicle Bridge facing upwest side. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. View of Clark Fork Vehicle Bridge facing up-west side. Looking at structural connection of top chord, vertical laced channel and diagonal bars. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

13. View of Clark Fork Vehicle Bridge facing south. Concrete ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. View of Clark Fork Vehicle Bridge facing south. Concrete barrier blocks access. Plaque was originally located where strioed traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

12. View of Clark Fork Vehicle Bridge facing south. Approach ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. View of Clark Fork Vehicle Bridge facing south. Approach from the north road. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability.

PubMed

Schalbetter, Stephanie A; Mansoubi, Sahar; Chambers, Anna L; Downs, Jessica A; Baxter, Jonathan

2015-08-18

Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA precatenation formed. Although fork rotation predominates at replication termination, in vitro studies have suggested that it also occurs frequently during elongation. However, the factors that influence fork rotation and how rotation and precatenation may influence other replication-associated processes are unknown. Here we analyze the causes and consequences of fork rotation in budding yeast. We find that fork rotation and precatenation preferentially occur in contexts that inhibit topoisomerase action ahead of the fork, including stable protein-DNA fragile sites and termination. However, generally, fork rotation and precatenation are actively inhibited by Timeless/Tof1 and Tipin/Csm3. In the absence of Tof1/Timeless, excessive fork rotation and precatenation cause extensive DNA damage following DNA replication. With Tof1, damage related to precatenation is focused on the fragile protein-DNA sites where fork rotation is induced. We conclude that although fork rotation and precatenation facilitate unwinding in hard-to-replicate contexts, they intrinsically disrupt normal chromosome duplication and are therefore restricted by Timeless/Tipin.

14. View of Clark Fork Vehicle Bridge facing north. Approach ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. View of Clark Fork Vehicle Bridge facing north. Approach from the south. Concrete barrier blocks access. Plaque was originally located where striped traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

40 CFR 131.33 - Idaho.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Creek, Doe Creek, Duck Creek, East Fork Holy Terror Creek, Fawn Creek, Flume Creek, Fly Creek, Forge... Terror Creek, J Fell Creek, Jacobs Ladder Creek, Lewis Creek, Liberty Creek, Lick Creek, Lime Creek... Creek, South Fork Chamberlain Creek, South Fork Holy Terror Creek, South Fork Norton Creek, South Fork...

23. Station Compressor Room 1 with Air Compressors and Accumulator ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. Station Compressor Room 1 with Air Compressors and Accumulator Tanks, view to the south. One of the two large station air compressor units used for depressing the draft tube water level is visible atop a concrete pedestal on the left side of photograph (the second identical compressor is located in an adjacent room). Two of the six station air accumulator tanks are visible in the background. The smaller station service air compressor is visible in right foreground of the photograph was installed in the early 1980s, and replaced the original station service air compressor. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

Endonuclease EEPD1 Is a Gatekeeper for Repair of Stressed Replication Forks*

PubMed Central

Kim, Hyun-Suk; Nickoloff, Jac A.; Wu, Yuehan; Williamson, Elizabeth A.; Sidhu, Gurjit Singh; Reinert, Brian L.; Jaiswal, Aruna S.; Srinivasan, Gayathri; Patel, Bhavita; Kong, Kimi; Burma, Sandeep; Lee, Suk-Hee; Hromas, Robert A.

2017-01-01

Replication is not as continuous as once thought, with DNA damage frequently stalling replication forks. Aberrant repair of stressed replication forks can result in cell death or genome instability and resulting transformation to malignancy. Stressed replication forks are most commonly repaired via homologous recombination (HR), which begins with 5′ end resection, mediated by exonuclease complexes, one of which contains Exo1. However, Exo1 requires free 5′-DNA ends upon which to act, and these are not commonly present in non-reversed stalled replication forks. To generate a free 5′ end, stalled replication forks must therefore be cleaved. Although several candidate endonucleases have been implicated in cleavage of stalled replication forks to permit end resection, the identity of such an endonuclease remains elusive. Here we show that the 5′-endonuclease EEPD1 cleaves replication forks at the junction between the lagging parental strand and the unreplicated DNA parental double strands. This cleavage creates the structure that Exo1 requires for 5′ end resection and HR initiation. We observed that EEPD1 and Exo1 interact constitutively, and Exo1 repairs stalled replication forks poorly without EEPD1. Thus, EEPD1 performs a gatekeeper function for replication fork repair by mediating the fork cleavage that permits initiation of HR-mediated repair and restart of stressed forks. PMID:28049724

24. View of one of the plaques from Clark Fork ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. View of one of the plaques from Clark Fork Vehicle Bridge. Presently located at the Bonner County Historical Museum in Sandpoint, Idaho. A plaque was attached at each end of the bridge. Only one remains. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

77 FR 76420 - Final Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-28

... Fort Gay, confluence. Unincorporated Areas of Wayne County. At the Tug Fork +575 confluence. Mill Creek (backwater effects from From the Tug Fork +575 Town of Fort Gay. Tug Fork). confluence to approximately 1.1 miles upstream of the Tug Fork confluence. Tug Fork At the Big Sandy River +575 Town of Fort Gay...

Seasonal variation of macrolide resistance gene abundances in the South Fork Iowa River Watershed

USDA-ARS?s Scientific Manuscript database

The Midwestern United States is dominated by agricultural production with high concentrations of swine, leading to application of swine manure onto lands with artificial subsurface drainage. Previous reports have indicated elevated levels of antibiotic resistance genes (ARGs) in surface water and gr...

Publications - GMC 7 | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

the source rock of Standard Oil Company of California North Fork Unit #41-35 Authors: Valentine, Grant , and Shell Oil Company Publication Date: 1976 Publisher: Alaska Division of Geological & for more information. Bibliographic Reference Valentine, Grant, and Shell Oil Company, 1976, Vitrinite

Continuous hydrologic simulation of runoff for the Middle Fork and South Fork of the Beargrass Creek basin in Jefferson County, Kentucky

USGS Publications Warehouse

Jarrett, G. Lynn; Downs, Aimee C.; Grace-Jarrett, Patricia A.

1998-01-01

The Hydrological Simulation Pro-gram-FORTRAN (HSPF) was applied to an urban drainage basin in Jefferson County, Ky to integrate the large amounts of information being collected on water quantity and quality into an analytical framework that could be used as a management and planning tool. Hydrologic response units were developed using geographic data and a K-means analysis to characterize important hydrologic and physical factors in the basin. The Hydrological Simulation Program FORTRAN Expert System (HSPEXP) was used to calibrate the model parameters for the Middle Fork Beargrass Creek Basin for 3 years (June 1, 1991, to May 31, 1994) of 5-minute streamflow and precipitation time series, and 3 years of hourly pan-evaporation time series. The calibrated model parameters were applied to the South Fork Beargrass Creek Basin for confirmation. The model confirmation results indicated that the model simulated the system within acceptable tolerances. The coefficient of determination and coefficient of model-fit efficiency between simulated and observed daily flows were 0.91 and 0.82, respectively, for model calibration and 0.88 and 0.77, respectively, for model confirmation. The model is most sensitive to estimates of the area of effective impervious land in the basin; the spatial distribution of rain-fall; and the lower-zone evapotranspiration, lower-zone nominal storage, and infiltration-capacity parameters during recession and low-flow periods. The error contribution from these sources varies with season and antecedent conditions.

Both DNA Polymerases δ and ε Contact Active and Stalled Replication Forks Differently

PubMed Central

Yu, Chuanhe; Gan, Haiyun

2017-01-01

ABSTRACT Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase with active and stalled replication forks. Here, we show that each DNA polymerase has a distinct pattern of association with active and stalled replication forks. Pol α is enriched at extending Okazaki fragments of active and stalled forks. In contrast, although Pol δ contacts the nascent lagging strands of active and stalled forks, it binds to only the matured (and not elongating) Okazaki fragments of stalled forks. Pol ε has greater contact with the nascent single-stranded DNA (ssDNA) of the leading strand on active forks than on stalled forks. We propose that the configuration of DNA polymerases at stalled forks facilitates the resumption of DNA synthesis after stress removal. PMID:28784720

The fecundity of fork-tailed threadfin bream (Nemipterus furcosus) in Bangka, Bangka Belitung

NASA Astrophysics Data System (ADS)

Utami, E.; Safitriyani, E.; Gatra Persada, Leo

2018-04-01

Fork-tailed threadfin bream (Nemipterus furcosus) is one of important economic fishes in Bangka. The sustainability of fork-tailed threadfin bream is threatened by degradation of natural habitat. Information of reproductive is needed for further management. The objective of this study was to examine fecundity of fork-tailed threadfin bream. The mean values of temperature was 28.83 ± 0,37°C, respectively. Sex ratio during sampling showed that female fork-tailed threadfin bream greater than male population. Berried female fork-tailed threadfin bream found from March until November. The greatest number of berried female fork-tailed threadfin bream showed in July with berried female value of 25. Fork-tailed threadfin bream fecundity was 19951 and 66628, respectively. The fecundity data can be used to access the reproductive potential of fish stock and also as an assessment on stock size of their natural population.

Inter-Fork Strand Annealing causes genomic deletions during the termination of DNA replication.

PubMed

Morrow, Carl A; Nguyen, Michael O; Fower, Andrew; Wong, Io Nam; Osman, Fekret; Bryer, Claire; Whitby, Matthew C

2017-06-06

Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. Replication fork barriers are a commonly encountered problem, which can cause fork collapse and act as hotspots for replication termination. Collapsed forks can be rescued by homologous recombination, which restarts replication. However, replication restart is relatively slow and, therefore, replication termination may frequently occur by an active fork converging on a collapsed fork. We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. Based on our findings, we propose that IFSA is a potential threat to genomic stability in eukaryotes.

Termination of DNA replication forks: "Breaking up is hard to do".

PubMed

Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

2015-01-01

To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component - Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field.

11. Photocopy of photograph (original copy in Edison collection). Photographer ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Photocopy of photograph (original copy in Edison collection). Photographer and date unknown, although photo taken prior to 1930 reconstruction of Project flumes. VIEW OF ORIGINAL SOUTH FORK OF THE TULE RIVER MIDDLE FORK "BOX" WOOD FLUME BRANCH SHOWING NORTH FORK OF TULE RIVER MIDDLE FORK CROSSING. VIEW TO NORTHWEST. - Tule River Hydroelectric Project, Water Conveyance System, Middle Fork Tule River, Springville, Tulare County, CA

Physical habitat classification and instream flow modeling to determine habitat availability during low-flow periods, North Fork Shenandoah River, Virginia

USGS Publications Warehouse

Krstolic, Jennifer L.; Hayes, Donald C.; Ruhl, Peter M.

2006-01-01

Increasing development and increasing water withdrawals for public, industrial, and agricultural water supply threaten to reduce streamflows in the Shenandoah River basin in Virginia. Water managers need more information to balance human water-supply needs with the daily streamflows necessary for maintaining the aquatic ecosystems. To meet the need for comprehensive information on hydrology, water supply, and instream-flow requirements of the Shenandoah River basin, the U.S. Geological Survey and the Northern Shenandoah Valley Regional Commission conducted a cooperative investigation of habitat availability during low-flow periods on the North Fork Shenandoah River. Historic streamflow data and empirical data on physical habitat, river hydraulics, fish community structure, and recreation were used to develop a physical habitat simulation model. Hydraulic measurements were made during low, medium, and high flows in six reaches at a total of 36 transects that included riffles, runs, and pools, and that had a variety of substrates and cover types. Habitat suitability criteria for fish were developed from detailed fish-community sampling and microhabitat observations. Fish were grouped into four guilds of species and life stages with similar habitat requirements. Simulated habitat was considered in the context of seasonal flow regimes to show the availability of flows that sustain suitable habitat during months when precipitation and streamflow are scarce. The North Fork Shenandoah River basin was divided into three management sections for analysis purposes: the upper section, middle section, and lower section. The months of July, August, and September were chosen to represent a low-flow period in the basin with low mean monthly flows, low precipitation, high temperatures, and high water withdrawals. Exceedance flows calculated from the combined data from these three months describe low-flow periods on the North Fork Shenandoah River. Long-term records from three streamflow-gaging stations were used to characterize the flow regime: North Fork Shenandoah River at Cootes Store, Va. (1925-2002), North Fork Shenandoah River at Mount Jackson, Va. (1943-2002), and North Fork Shenandoah River near Strasburg, Va. (1925-2002). The predominant mesohabitat types (14 percent riffle, 67.3 percent run, and 18.7 percent pool) were classified along the entire river (100 miles) to assist in the selection of reaches for hydraulic and fish community data collection. The upper section has predominantly particle substrate, ranging in size from sand to boulders, and the shortest habitat units. The middle section is a transitional section with increased bedrock substrate and habitat unit length. The lower section has predominantly bedrock substrate and the longest habitat units in the river. The model simulations show that weighted usable-habitat area in the upper management section is highest at flows higher than the 25-percent exceedance flow for July, August, and September. During these three months, total weighted usable-habitat area in this section is often less than the simulated maximum weighted usable-habitat area. Habitat area in the middle management section is highest at flows between the 25- and 75-percent exceedance flows for July, August, and September. In the middle section during these months, both the actual weighted usable-habitat area and the simulated maximum weighted usable-habitat area are associated with this flow range. Weighted usable-habitat area in the lower management section is highest at flows lower than the 75-percent exceedance flow for July, August, and September. In the lower section during these three months, some weighted usable-habitat area is available, but the normal range of flows does not include the simulated maximum weighted usable-habitat area. A time-series habitat analysis associated with the historic streamflow, zero water withdrawals, and doubled water withdrawals was completed. During s

Grand Forks/East Grand Forks ITS strategy plan

DOT National Transportation Integrated Search

2001-01-15

The Grand Forks/East Grand Forks (GF/EGF) Area's Intelligent Transportation Systems (ITS) Strategy Plan is an effort by the GF/EGF Metropolitan Planning Organization (MPO) and its partners to develop a plan for deploying Intelligent Transportation Sy...

77 FR 39675 - Wallowa-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-05

...-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining AGENCY: Forest Service, USDA... North Fork Burnt River Mining Record of Decision will replace and supercede the 2004 North Fork Burnt...

Replication stress-induced chromosome breakage is correlated with replication fork progression and is preceded by single-stranded DNA formation.

PubMed

Feng, Wenyi; Di Rienzi, Sara C; Raghuraman, M K; Brewer, Bonita J

2011-10-01

Chromosome breakage as a result of replication stress has been hypothesized to be the direct consequence of defective replication fork progression, or "collapsed" replication forks. However, direct and genome-wide evidence that collapsed replication forks give rise to chromosome breakage is still lacking. Previously we showed that a yeast replication checkpoint mutant mec1-1, after transient exposure to replication impediment imposed by hydroxyurea (HU), failed to complete DNA replication, accumulated single-stranded DNA (ssDNA) at the replication forks, and fragmented its chromosomes. In this study, by following replication fork progression genome-wide via ssDNA detection and by direct mapping of chromosome breakage after HU exposure, we have tested the hypothesis that the chromosome breakage in mec1 cells occurs at collapsed replication forks. We demonstrate that sites of chromosome breakage indeed correlate with replication fork locations. Moreover, ssDNA can be detected prior to chromosome breakage, suggesting that ssDNA accumulation is the common precursor to double strand breaks at collapsed replication forks.

Baseline geochemical data for stream sediment and surface water samples from Panther Creek, the Middle Fork of the Salmon River, and the Main Salmon River from North Fork to Corn Creek, collected prior to the severe wildfires of 2000 in central Idaho

USGS Publications Warehouse

Eppinger, Robert G.; Briggs, Paul H.; Brown, Zoe Ann; Crock, James G.; Meier, Allen; Theodorakos, Peter M.; Wilson, Stephen A.

2001-01-01

In 1996, the U.S. Geological Survey conducted a reconnaissance baseline geochemical study in central Idaho. The purpose of the baseline study was to establish a 'geochemical snapshot' of the area, as a datum for monitoring future change in the geochemical landscape, whether natural or human-induced. This report presents the methology, analytical results, and sample descriptions for water, sediment, and heavy-mineral concentrate samples collected during this geochemical investigation. In the summer of 2000, the Clear Creek, Little Pistol, and Shellrock wildfires swept across much of the area that was sampled. Thus, these data represent a pre-fire baseline geochemical dataset. A 2001 post- fire study is planned and will involve re-sampling of the pre-fire baseline sites, to allow for pre- and post-fire comparison.

FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5′-DNA end

PubMed Central

Sato, Koichi; Shimomuki, Mayo; Katsuki, Yoko; Takahashi, Daisuke; Kobayashi, Wataru; Ishiai, Masamichi; Miyoshi, Hiroyuki; Takata, Minoru; Kurumizaka, Hitoshi

2016-01-01

The FANCI-FANCD2 (I-D) complex is considered to work with RAD51 to protect the damaged DNA in the stalled replication fork. However, the means by which this DNA protection is accomplished have remained elusive. In the present study, we found that the I-D complex directly binds to RAD51, and stabilizes the RAD51-DNA filament. Unexpectedly, the DNA binding activity of FANCI, but not FANCD2, is explicitly required for the I-D complex-mediated RAD51-DNA filament stabilization. The RAD51 filament stabilized by the I-D complex actually protects the DNA end from nucleolytic degradation by an FA-associated nuclease, FAN1. This DNA end protection is not observed with the RAD51 mutant from FANCR patient cells. These results clearly answer the currently enigmatic question of how RAD51 functions with the I-D complex to prevent genomic instability at the stalled replication fork. PMID:27694619

General perspective view of the North Fork Butter Creek Bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

General perspective view of the North Fork Butter Creek Bridge, view looking southwest - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Approach view of the North Fork Butter Creek Bridge, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Approach view of the North Fork Butter Creek Bridge, view looking south - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

General perspective view of the North Fork Butter Creek Bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

General perspective view of the North Fork Butter Creek Bridge, view looking north - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Elevation view of the North Fork Butter Creek Bridge, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Elevation view of the North Fork Butter Creek Bridge, view looking west - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Approach view of the North Fork Butter Creek Bridge, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Approach view of the North Fork Butter Creek Bridge, view looking north - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Detail perspective view of the North Fork Butter Creek Bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail perspective view of the North Fork Butter Creek Bridge, view looking southwest - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

General perspective view of the North Fork Butter Creek Bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

General perspective view of the North Fork Butter Creek Bridge, view looking south - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Assessment of hand-transmitted vibration exposure from motorized forks used for beach-cleaning operations.

PubMed

McDowell, Thomas W; Welcome, Daniel E; Warren, Christopher; Xu, Xueyan S; Dong, Ren G

2013-01-01

Motorized vibrating manure forks were used in beach-cleaning operations following the massive Deepwater Horizon oil spill in the Gulf of Mexico during the summer of 2010. The objectives of this study were to characterize the vibration emissions of these motorized forks and to provide a first approximation of hand-transmitted vibration exposures to workers using these forks for beach cleaning. Eight operators were recruited to operate the motorized forks during this laboratory study. Four fork configurations were used in the study; two motor speeds and two fork basket options were evaluated. Accelerations were measured near each hand as the operators completed the simulated beach-cleaning task. The dominant vibration frequency for these tools was identified to be around 20 Hz. Because acceleration was found to increase with motor speed, workers should consider operating these tools with just enough speed to get the job done. These forks exhibited considerable acceleration magnitudes when unloaded. The study results suggest that the motor should not be operated with the fork in the unloaded state. Anti-vibration gloves are not effective at attenuating the vibration frequencies produced by these forks, and they may even amplify the transmitted vibration and increase hand/arm fatigue. While regular work gloves are suitable, vibration-reducing gloves may not be appropriate for use with these tools. These considerations may also be generally applicable for the use of motorized forks in other workplace environments.

Factors influencing stream fish recovery following a large-scale disturbance

Treesearch

William E. Ensign; Angermeier Leftwich; C. Andrew Dolloff

1997-01-01

The authors examined fish distribution and abundance in erosional habitat units in South Fork Roanoke River, VA, following a fish kill by using a reachwide sampling approach for 3 species and a representative-reach sampling approach for 10 species. Qualitative (presence-absence) and quantitative (relative abundance) estimates of distribution and abundance provided...

Multiscale thermal refugia and stream habitat associations of chinook salmon in northwestern Oregon

USGS Publications Warehouse

Torgersen, Christian E.; Price, David M.; Li, Hiram W.; McIntosh, B.A.

1999-01-01

We quantified distribution and behavior of adult spring chinook salmon (Oncorhynchus tshawytscha) related to patterns of stream temperature and physical habitat at channel-unit, reach-, and section-level spatial scales in a wilderness stream and a disturbed stream in the John Day River basin in northeastern Oregon. We investigated the effectiveness of thermal remote sensing for analyzing spatial patterns of stream temperature and assessed habitat selection by spring chinook salmon, evaluating whether thermal refugia might be responsible for the persistence of these stocks in rivers where water temperatures frequently exceed their upper tolerance levels (25A?C) during spawning migration. By presenting stream temperature and the ecology of chinook salmon in a historical context, we could evaluate how changes in riverine habitat and thermal spatial structure, which can be caused by land-use practices, may influence distributional patterns of chinook salmon. Thermal remote sensing provided spatially continuous maps of stream temperature for reaches used by chinook salmon in the upper subbasins of the Middle Fork and North Fork John Day River. Electivity analysis and logistic regression were used to test for associations between the longitudinal distribution of salmon and cool-water areas and stream habitat characteristics. Chinook salmon were distributed nonuniformly in reaches throughout each stream. Salmon distribution and cool water temperature patterns were most strongly related at reach-level spatial scales in the warm stream, the Middle Fork (maximum likelihood ratio: P 0.30). Pools were preferred by adult chinook salmon in both subbasins (Bonferroni confidence interval: P a?? 0.05); however, riffles were used proportionately more frequently in the North Fork than in the Middle Fork. Our observations of thermal refugia and their use by chinook salmon at multiple spatial scales reveal that, although heterogeneity in the longitudinal stream temperature profile may be viewed as an ecological warning sign, thermal patchiness in streams also should be recognized for its biological potential to provide habitat for species existing at the margin of their environmental tolerances.

Note: Enhanced energy harvesting from low-frequency magnetic fields utilizing magneto-mechano-electric composite tuning-fork.

PubMed

Yang, Aichao; Li, Ping; Wen, Yumei; Yang, Chao; Wang, Decai; Zhang, Feng; Zhang, Jiajia

2015-06-01

A magnetic-field energy harvester using a low-frequency magneto-mechano-electric (MME) composite tuning-fork is proposed. This MME composite tuning-fork consists of a copper tuning fork with piezoelectric Pb(Zr(1-x)Ti(x))O3 (PZT) plates bonded near its fixed end and with NdFeB magnets attached at its free ends. Due to the resonance coupling between fork prongs, the MME composite tuning-fork owns strong vibration and high Q value. Experimental results show that the proposed magnetic-field energy harvester using the MME composite tuning-fork exhibits approximately 4 times larger maximum output voltage and 7.2 times higher maximum power than the conventional magnetic-field energy harvester using the MME composite cantilever.

Topographic view of the North Fork Butter Creek Bridge (located ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Topographic view of the North Fork Butter Creek Bridge (located center of frame), view looking west - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks.

PubMed

Elia, Andrew E H; Wang, David C; Willis, Nicholas A; Boardman, Alexander P; Hajdu, Ildiko; Adeyemi, Richard O; Lowry, Elizabeth; Gygi, Steven P; Scully, Ralph; Elledge, Stephen J

2015-10-15

We have used quantitative proteomics to profile ubiquitination in the DNA damage response (DDR). We demonstrate that RPA, which functions as a protein scaffold in the replication stress response, is multiply ubiquitinated upon replication fork stalling. Ubiquitination of RPA occurs on chromatin, involves sites outside its DNA binding channel, does not cause proteasomal degradation, and increases under conditions of fork collapse, suggesting a role in repair at stalled forks. We demonstrate that the E3 ligase RFWD3 mediates RPA ubiquitination. RFWD3 is necessary for replication fork restart, normal repair kinetics during replication stress, and homologous recombination (HR) at stalled replication forks. Mutational analysis suggests that multisite ubiquitination of the entire RPA complex is responsible for repair at stalled forks. Multisite protein group sumoylation is known to promote HR in yeast. Our findings reveal a similar requirement for multisite protein group ubiquitination during HR at stalled forks in mammalian cells. Copyright © 2015 Elsevier Inc. All rights reserved.

Feasibility and safety of modified inverted T-shaped method using linear stapler with movable cartridge fork for esophagojejunostomy following laparoscopic total gastrectomy.

PubMed

Ohuchida, Kenoki; Nagai, Eishi; Moriyama, Taiki; Shindo, Koji; Manabe, Tatsuya; Ohtsuka, Takao; Shimizu, Shuji; Nakamura, Masafumi

2017-01-01

We previously reported the use of an inverted T-shaped method to obtain a suitable view for hand sewing to close the common entry hole when the linear stapler was fired for esophagojejunostomy after laparoscopic total gastrectomy (LTG). This conventional method involved insertion of the fixed cartridge fork to the Roux limb and the fine movable anvil fork to the esophagus to avoid perforation of the jejunum. However, insertion of the movable anvil fork to the esophagus during this procedure often requires us to strongly push down the main body of the stapler with the fixed cartridge fork to bring the direction of the anvil fork in line with the direction of the long axis of the esophagus while controlling the opening of the movable anvil fork. We therefore modified this complicated inverted T-shaped method using a linear stapler with a movable cartridge fork. This modified method involved insertion of the movable cartridge fork into the Roux limb followed by natural, easy insertion of the fixed anvil fork into the esophagus without controlling the opening of the movable cartridge fork. We performed LTG in a total of 155 consecutive patients with gastric cancer from November 2007 to December 2015 in Kyushu University Hospital. After LTG, we performed the conventional inverted T-shaped method using a linear stapler with a fixed cartridge fork in 61 patients from November 2007 to July 2011 (fixed cartridge group). From August 2011, we used a linear stapler with a movable cartridge fork and performed the modified inverted T-shaped method in 94 patients (movable cartridge group). We herein compare the short-term outcomes in 94 cases of LTG using the modified method (movable cartridge fork) with those in 61 cases using the conventional method (fixed cartridge fork). We found no significant differences in the perioperative or postoperative events between the movable and fixed cartridge groups. One case of anastomotic leakage occurred in the fixed cartridge group, but no anastomotic leakage occurred in the movable cartridge group. Although there were no remarkable differences in the short-term outcomes between the movable and fixed cartridge groups, we believe that the modified inverted T-shaped method is technically more feasible and reliable than the conventional method and will contribute to the improved safety of LTG.

Feasibility and safety of modified inverted T-shaped method using linear stapler with movable cartridge fork for esophagojejunostomy following laparoscopic total gastrectomy

PubMed Central

Ohuchida, Kenoki; Moriyama, Taiki; Shindo, Koji; Manabe, Tatsuya; Ohtsuka, Takao; Shimizu, Shuji; Nakamura, Masafumi

2017-01-01

Background We previously reported the use of an inverted T-shaped method to obtain a suitable view for hand sewing to close the common entry hole when the linear stapler was fired for esophagojejunostomy after laparoscopic total gastrectomy (LTG). This conventional method involved insertion of the fixed cartridge fork to the Roux limb and the fine movable anvil fork to the esophagus to avoid perforation of the jejunum. However, insertion of the movable anvil fork to the esophagus during this procedure often requires us to strongly push down the main body of the stapler with the fixed cartridge fork to bring the direction of the anvil fork in line with the direction of the long axis of the esophagus while controlling the opening of the movable anvil fork. We therefore modified this complicated inverted T-shaped method using a linear stapler with a movable cartridge fork. This modified method involved insertion of the movable cartridge fork into the Roux limb followed by natural, easy insertion of the fixed anvil fork into the esophagus without controlling the opening of the movable cartridge fork. Methods We performed LTG in a total of 155 consecutive patients with gastric cancer from November 2007 to December 2015 in Kyushu University Hospital. After LTG, we performed the conventional inverted T-shaped method using a linear stapler with a fixed cartridge fork in 61 patients from November 2007 to July 2011 (fixed cartridge group). From August 2011, we used a linear stapler with a movable cartridge fork and performed the modified inverted T-shaped method in 94 patients (movable cartridge group). We herein compare the short-term outcomes in 94 cases of LTG using the modified method (movable cartridge fork) with those in 61 cases using the conventional method (fixed cartridge fork). Results We found no significant differences in the perioperative or postoperative events between the movable and fixed cartridge groups. One case of anastomotic leakage occurred in the fixed cartridge group, but no anastomotic leakage occurred in the movable cartridge group. Conclusions Although there were no remarkable differences in the short-term outcomes between the movable and fixed cartridge groups, we believe that the modified inverted T-shaped method is technically more feasible and reliable than the conventional method and will contribute to the improved safety of LTG. PMID:28616606

[History of the tuning fork. I: Invention of the tuning fork, its course in music and natural sciences. Pictures from the history of otorhinolaryngology, presented by instruments from the collection of the Ingolstadt German Medical History Museum].

PubMed

Feldmann, H

1997-02-01

G. Cardano, physician, mathematician, and astrologer in Pavia, Italy, in 1550 described how sound may be perceived through the skull. A few years later H. Capivacci, also a physician in Padua, realized that this phenomenon might be used as a diagnostic tool for differentiating between hearing disorders located either in the middle ear or in the acoustic nerve. The German physician G. C. Schelhammer in 1684 was the first to use a common cutlery fork in further developing the experiments initiated by Cardano and Capivacci. For a long time to come, however, there was no demand for this in practical otology. The tuning fork was invented in 1711 by John Shore, trumpeter and lutenist to H. Purcell and G.F. Händel in London. A picture of Händel's own tuning fork, probably the oldest tuning fork in existence, is presented here for the first time. There are a number of anecdotes connected with the inventor of the tuning fork, using plays on words involving the name Shore, and mixing up pitch-pipe and pitchfork. Some of these are related here. The tuning fork as a musical instrument soon became a success throughout Europe. The German physicist E. F. F. Chladni in Wittenberg around 1800 was the first to systematically investigate the mode of vibration of the tuning fork with its nodal points. Besides this, he and others tried to construct a complete musical instrument based on sets of tuning forks, which, however, were not widely accepted. J. H. Scheibler in Germany in 1834 presented a set of 54 tuning forks covering the range from 220 Hz to 440 Hz, at intervals of 4 Hz. J. Lissajous in Paris constructed a very elaborate tuning fork with a resonance box, which was intended to represent the international standard of the musical note A with 435 vibrations per second, but this remained controversial. K. R. Koenig, a German physicist living in Paris, invented a tuning fork which was kept in continuous vibration by a clockwork. H. Helmholtz, physiologist in Heidelberg, in 1863 used sets of electromagnetically powered tuning forks for his famous experiments on the sensations of tone. Until the invention of the electronic valve, tuning forks remained indispensible instruments for producing defined sinusoidal vibrations. The history of this development is presented in detail. The diagnostic use of the tuning fork in otology will be described in a separate article.

Water quality of Fremont Lake and New Fork Lakes, western Wyoming; a progress report

USGS Publications Warehouse

Peterson, D.A.; Averett, R.C.; Mora, K.L.

1987-01-01

Fremont Lake and New Fork Lakes in the New Fork River drainage of western Wyoming were selected for a comprehensive study of hydrologic processes affecting mountain lakes in the Rocky Mountains. Information is needed about lakes in this area to assess their response to existing and planned development. The concerns include regional issues such as acid precipitation from gas-sweetening plants, coal-fired powerplants, and smelters, as well as local issues, such as shoreline development and raising outlet control structures. Onsite measurements indicated strong thermal stratification in the lakes during the summer. Isothermal conditions occurred during December 1983 and May 1984. Mean phytoplankton concentrations were less than 5,000 cells/ml, and chlorophyll a concentrations were weakly correlated with phytoplankton concentrations. Zooplankton concentrations were small, less than 6 organisms/L. The numbers of benthic invertebrates/unit area in Fremont Lake were extremely small. The lake waters and inflow and outflow streams were chemically dilute solutions. Mean dissolved-solids concentrations were 13 mg/L in Fremont Lake and 24 mg/L in New Fork Lakes. Calcium and bicarbonate were the predominant ions. Concentrations of phosphorus and nitrogen usually were less than detection limits. Trace-metals concentrations in the lakes were similar to those in precipitation and generally were small. Dissolved organic-carbon concentrations were about 1 mg/L. Concentrations of fulvic and humic acids were relatively large in the inlet of Fremont Lake during the spring. Pine Creek has deposited 800 metric tons of sediment, on an annual average, to the delta of Fremont Lake. Most sediment is deposited during spring runoff. (USGS)

21 CFR 882.1525 - Tuning fork.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning fork...

21 CFR 882.1525 - Tuning fork.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning fork...

36 CFR 292.62 - Valid existing rights.

Code of Federal Regulations, 2010 CFR

2010-07-01

... RECREATION AREAS Smith River National Recreation Area § 292.62 Valid existing rights. (a) Definition. For the... “wild” segments of the Wild and Scenic Smith River, Middle Fork Smith River, North Fork Smith River, Siskiyou Fork Smith River, South Fork Smith River, and their designated tributaries, except Peridotite...

Perspectives from the Third International Summit on Medical Nutrition Education and Research

PubMed Central

Crowley, Jennifer Jean; Laur, Celia; Carter, Harrison David Edward; Jones, Glenys; Ray, Sumantra

2018-01-01

Nutrition is an important component of public health and health care, including in education and research, and in the areas of policy and practice. This statement was the overarching message during the third annual International Summit on Medical Nutrition Education and Research, held at Wolfson College, University of Cambridge, United Kingdom, in August 2017. This summit encouraged attendees to think more broadly about the impact of nutrition policy on health and communities, including the need to visualize the complete food system from “pre-farm to post-fork.” Evidence of health issues related to food and nutrition were presented, including the need for translation of knowledge into policy and practice. Methods for this translation included the use of implementation and behavior change techniques, recognizing the needs of health-care professionals, policy makers, and the public. In all areas of nutrition and health, clear and effective messages, supported by open data, information, and actionable knowledge, are also needed along with strong measures of impact centered on an ultimate goal: to improve nutritional health and wellbeing for patients and the public. PMID:29629367

Perspectives from the Third International Summit on Medical Nutrition Education and Research.

PubMed

Crowley, Jennifer Jean; Laur, Celia; Carter, Harrison David Edward; Jones, Glenys; Ray, Sumantra

2018-01-01

Nutrition is an important component of public health and health care, including in education and research, and in the areas of policy and practice. This statement was the overarching message during the third annual International Summit on Medical Nutrition Education and Research, held at Wolfson College, University of Cambridge, United Kingdom, in August 2017. This summit encouraged attendees to think more broadly about the impact of nutrition policy on health and communities, including the need to visualize the complete food system from "pre-farm to post-fork." Evidence of health issues related to food and nutrition were presented, including the need for translation of knowledge into policy and practice. Methods for this translation included the use of implementation and behavior change techniques, recognizing the needs of health-care professionals, policy makers, and the public. In all areas of nutrition and health, clear and effective messages, supported by open data, information, and actionable knowledge, are also needed along with strong measures of impact centered on an ultimate goal: to improve nutritional health and wellbeing for patients and the public.

Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism

PubMed Central

Reynolds, John J; Bicknell, Louise S; Carroll, Paula; Higgs, Martin R; Shaheen, Ranad; Murray, Jennie E; Papadopoulos, Dimitrios K; Leitch, Andrea; Murina, Olga; Tarnauskaitė, Žygimantė; Wessel, Sarah R; Zlatanou, Anastasia; Vernet, Audrey; von Kriegsheim, Alex; Mottram, Rachel MA; Logan, Clare V; Bye, Hannah; Li, Yun; Brean, Alexander; Maddirevula, Sateesh; Challis, Rachel C; Skouloudaki, Kassiani; Almoisheer, Agaadir; Alsaif, Hessa S; Amar, Ariella; Prescott, Natalie J; Bober, Michael B; Duker, Angela; Faqeih, Eissa; Seidahmed, Mohammed Zain; Al Tala, Saeed; Alswaid, Abdulrahman; Ahmed, Saleem; Al-Aama, Jumana Yousuf; Altmüller, Janine; Al Balwi, Mohammed; Brady, Angela F; Chessa, Luciana; Cox, Helen; Fischetto, Rita; Heller, Raoul; Henderson, Bertram D; Hobson, Emma; Nürnberg, Peter; Percin, E Ferda; Peron, Angela; Spaccini, Luigina; Quigley, Alan J; Thakur, Seema; Wise, Carol A; Yoon, Grace; Alnemer, Maha; Tomancak, Pavel; Yigit, Gökhan; Taylor, A Malcolm R; Reijns, Martin AM; Simpson, Michael A; Cortez, David; Alkuraya, Fowzan S; Mathew, Christopher G; Jackson, Andrew P; Stewart, Grant S

2017-01-01

To ensure efficient genome duplication, cells have evolved numerous factors that promote unperturbed DNA replication, and protect, repair and restart damaged forks. Here we identify DONSON as a novel fork protection factor, and report biallelic DONSON mutations in 29 individuals with microcephalic dwarfism. We demonstrate that DONSON is a replisome component that stabilises forks during genome replication. Loss of DONSON leads to severe replication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks. Furthermore, ATR-dependent signalling in response to replication stress is impaired in DONSON-deficient cells, resulting in decreased checkpoint activity, and potentiating chromosomal instability. Hypomorphic mutations substantially reduce DONSON protein levels and impair fork stability in patient cells, consistent with defective DNA replication underlying the disease phenotype. In summary, we identify mutations in DONSON as a common cause of microcephalic dwarfism, and establish DONSON as a critical replication fork protein required for mammalian DNA replication and genome stability. PMID:28191891

Hydrology of the North Fork of the Right Fork of Miller Creek, Carbon County, Utah, before, during, and after underground coal mining

USGS Publications Warehouse

Slaughter, C.B.; Freethey, G.W.; Spangler, L.E.

1995-01-01

From 1988-92 the U.S. Geological Survey, in cooperation with the Utah Division of Oil, Gas, and Mining, studied the effects of underground coal mining and the resulting subsidence on the hydrologic system near the North Fork of the Right Fork of Miller Creek, Carbon County, Utah. The subsidence caused open fractures at land surface, debris slides, and rockfalls in the canyon above the mined area. Land surface subsided and moved several feet horizontally. The perennial stream and a tributary upstream from the mined area were diverted below the ground by surface fractures where the overburden thickness above the Wattis coal seam is 300 to 500 feet. The reach downstream was dry but flow resumed where the channel traversed the Star Point Sandstone, which forms the aquifer below the coal seams where ground-water discharge provides new base flow. Concentrations of dissolved constituents in the stream water sampled just downstream from the mined area increased from about 300 mg/L (milligrams per liter) to more than 1,500 mg/L, and the water changed from primarily a magnesium calcium bicarbonate to primarily a magnesium sulfate type. Monitored water levels in two wells completed in the perched aquifer(s) above the mine indicate that fractures from subsidence- related deformation drained the perched aquifer in the Blackhawk Formation. The deformation also could have contributed to the decrease in discharge of three springs above the mined area, but discharge from other springs in the area did not change ubstantially; thus, the relation between subsidence and spring discharge, if any, is not clear. No significant changes in the chemical character of water discharging from springs were detected, but the dissolved-solids concentration in water collected from a perched sandstone aquifer overlying the mined coal seams increased during mining activity.

High-affinity DNA-binding Domains of Replication Protein A (RPA) Direct SMARCAL1-dependent Replication Fork Remodeling*

PubMed Central

Bhat, Kamakoti P.; Bétous, Rémy; Cortez, David

2015-01-01

SMARCAL1 catalyzes replication fork remodeling to maintain genome stability. It is recruited to replication forks via an interaction with replication protein A (RPA), the major ssDNA-binding protein in eukaryotic cells. In addition to directing its localization, RPA also activates SMARCAL1 on some fork substrates but inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reactions. We investigated the mechanism by which RPA regulates SMARCAL1. Our results indicate that although an interaction between SMARCAL1 and RPA is essential for SMARCAL1 activation, the location of the interacting surface on RPA is not. Counterintuitively, high-affinity DNA binding of RPA DNA-binding domain (DBD) A and DBD-B near the fork junction makes it easier for SMARCAL1 to remodel the fork, which requires removing RPA. We also found that RPA DBD-C and DBD-D are not required for SMARCAL1 regulation. Thus, the orientation of the high-affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity. PMID:25552480

High-affinity DNA-binding domains of replication protein A (RPA) direct SMARCAL1-dependent replication fork remodeling.

PubMed

Bhat, Kamakoti P; Bétous, Rémy; Cortez, David

2015-02-13

SMARCAL1 catalyzes replication fork remodeling to maintain genome stability. It is recruited to replication forks via an interaction with replication protein A (RPA), the major ssDNA-binding protein in eukaryotic cells. In addition to directing its localization, RPA also activates SMARCAL1 on some fork substrates but inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reactions. We investigated the mechanism by which RPA regulates SMARCAL1. Our results indicate that although an interaction between SMARCAL1 and RPA is essential for SMARCAL1 activation, the location of the interacting surface on RPA is not. Counterintuitively, high-affinity DNA binding of RPA DNA-binding domain (DBD) A and DBD-B near the fork junction makes it easier for SMARCAL1 to remodel the fork, which requires removing RPA. We also found that RPA DBD-C and DBD-D are not required for SMARCAL1 regulation. Thus, the orientation of the high-affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Physical Habitat Characteristics on the North and South Forks of the Shenandoah River, VA in 2002-2007

USGS Publications Warehouse

Krstolic, Jennifer L.; Hayes, Donald C.

2010-01-01

Data collected with the GeoXT Trimble GPS unit using ArcPad 6.1. (summer 2006-2007). Files were created within a geodatabase to create a data dictionary for use in ArcPad during data collection. Drop down lists for habitat type, substrate, depth, width, length, and descriptions were included. Data files produced on the GeoXT were point shapefiles that could be checked back into the geodatabase and viewable as a layer. Points were gathered while canoeing along the South Fork Shenandoah River. Each location marked a change in meso-scale habitat type. GPS points were supplemented with GIS-derived points in areas where manual measurements were made. The points were used to generate a line coverage. This coverage represents physical habitat at a meso-scale (width of stream).

Runoff generation from neighboring headwater basins with differing glacier coverage using the distributed hydrological model WaSiM, Eklutna, Alaska

NASA Astrophysics Data System (ADS)

Ostman, J. S.; Loso, M.; Liljedahl, A. K.; Gaedeke, A.; Geck, J. E.

2017-12-01

Many Alaska glaciers are thinning and retreating, and glacier wastage is projected to affect runoff processes from glacierized basins. Accordingly, effective resource management in glacierized watersheds requires quantification of a glacier's role on streamflow generation. The Eklutna catchment (311 km2) supplies water and electricity for Anchorage, Alaska (pop. 300,000) via Eklutna Lake. The Eklutna headwaters include the West Fork (64 km2, 46% glacier), and the East Fork (101 km2, 12% glacier). Total average annual discharge (2009-2015) is similar from the West (42,100 m3) and East (42,200 m3) forks, while specific annual runoff from the West Fork (2940 mm) exceeds that of the East Fork (1500 mm). To better understand what controls runoff, we are simulating the Eklutna annual water budget using a distributed watershed-level hydrological model. We force the Water Flow and Balance Simulation Model (WaSiM) using continuous air temperature, precipitation, wind speed, shortwave incoming radiation, and relative humidity primarily measured in the West Fork basin. We use Eklutna Glacier snow accumulation and ablation to calibrate the snowmelt and glacier sub-modules. Melt season discharge from the West and East forks is used for runoff comparison. Preliminary results show 2013-2015 simulated glacier point balances (accumulation and melt) are within 15% of glacier stake observations. Runoff was effectively modeled in the West Fork (NSE=0.80), while being over-predicted in the East Fork , which we attribute to a lack of forcing data in the less-glacierized basin. The simulations suggest that 78% of West Fork total runoff is from glacier melt, compared with <40% in the East Fork where glacier runoff contribution is higher during low-snow years.

78 FR 26384 - Indian Entities Recognized and Eligible To Receive Services From the United States Bureau of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-06

...: (Barona Group of Capitan Grande Band of Mission Indians of the Barona Reservation, California; Viejas (Baron Long) Group of Capitan Grande Band of Mission Indians of the Viejas Reservation, California... Indians of Nevada (Four constituent bands: Battle Mountain Band; Elko Band; South Fork Band and Wells Band...

Aflatoxin contamination of dried red chilies: Contrasts between the United States and Nigeria, two markets differing in regulation enforcement

USDA-ARS?s Scientific Manuscript database

Dried red chilies are among the world’s most consumed spices. From farm to fork, chilies go through cropping, harvest, drying, processing and storage. Chilies are susceptible to infection by aflatoxin producing fungi and subsequent contamination by aflatoxins at every stage. Aflatoxins are highly re...

Consumer-Driven Health Care: Answer to Global Competition or Threat to Social Justice?

ERIC Educational Resources Information Center

Owen, Carol L.

2009-01-01

Health planning in the United States is rapidly approaching a fork in the policy road, with one direction leading the nation toward a universal plan with strong government involvement and the other direction strengthening existing market-based reforms and preserving a commercial health insurance industry. "Consumer-driven health care," a slogan…

76 FR 6114 - Lincoln National Forest, New Mexico, North Fork Eagle Creek Wells Special Use Authorization

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-03

... groundwater drawdown from this well field to maintain surface flows and protect water-dependent ecosystems.... The United States Geological Survey (USGS) conducted the independent study from 2007-2009 to determine... during both time periods, there were no days of zero flow recorded at the Eagle Creek gage from 1969-1980...

Movement of bull trout in the upper Jarbidge River watershed, Idaho and Nevada, 2008-09--A supplement to Open-File Report 2010-1033

USGS Publications Warehouse

Munz, Carrie S.; Allen, M. Brady; Connolly, Patrick J.

2011-01-01

We monitored bull trout (Salvelinus confluentus) in 2008 and 2009 as a continuation of our work in 2006 and 2007, which involved the tagging of 1,536 bull trout with passive integrated transponder (PIT) tags in the East Fork Jarbidge River and West Fork Jarbidge River and their tributaries in northeastern Nevada and southern Idaho. We installed PIT tag interrogation systems (PTISs) at established locations soon after ice-out, and maintained the PTISs in order to collect information on bull trout movements through December of each year. We observed a marked increase of movement in 2008 and 2009. Bull trout tagged in the uppermost portions of the East Fork Jarbidge River at altitudes greater than 2,100 meters moved to the confluence of the East Fork Jarbidge River and West Fork Jarbidge River in summer and autumn. Ten bull trout tagged upstream of the confluence of Pine Creek and the West Fork Jarbidge River moved downstream and then upstream in the East Fork Jarbidge River, and then past the PTIS at Murphy Hot Springs (river kilometer [rkm] 4.1). Two of these fish ascended Dave Creek, a tributary of the East Fork Jarbidge River, past the PTIS at rkm 0.4. One bull trout that was tagged at rkm 11 in Dave Creek on June 28, 2007 moved downstream to the confluence of the East Fork Jarbidge River and West Fork Jarbidge River (rkm 0) on July 28, 2007, and it was then detected in the West Fork Jarbidge River moving past our PTIS at rkm 15 on May 4, 2008. Combined, the extent and types of bull trout movements observed indicated that the primarily age-1 and age-2 bull trout that we tagged in 2006 and 2007 showed increased movement with age and evidence of a substantial amount of fluvial life history. The movements suggest strong connectivity between spawning areas and downstream mainstem areas, as well as between the East Fork Jarbidge River and West Fork Jarbidge River.

Salmon Supplementation Studies in Idaho Rivers, 1999-2000 Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kohler, Andy; Taki, Doug; Teton, Angelo

2001-11-01

As part of the Idaho Supplementation Studies, fisheries crews from the Shoshone-Bannock Tribes have been snorkeling tributaries of the Salmon River to estimate chinook salmon (Oncorhynchus tshawytscha) parr abundance; conducting surveys of spawning adult chinook salmon to determine the number of redds constructed and collect carcass information; operating a rotary screw trap on the East Fork Salmon River and West Fork Yankee Fork Salmon River to enumerate and PIT-tag emigrating juvenile chinook salmon; and collecting and PIT-tagging juvenile chinook salmon on tributaries of the Salmon River. The Tribes work in the following six tributaries of the Salmon River: Bear Valleymore » Creek, East Fork Salmon River, Herd Creek, South Fork Salmon River, Valley Creek, and West Fork Yankee Fork Salmon River. Snorkeling was used to obtain parr population estimates for ISS streams from 1992 to 1997. However, using the relatively vigorous methods described in the ISS experimental design to estimate summer chinook parr populations, results on a project-wide basis showed extraordinarily large confidence intervals and coefficients of variation. ISS cooperators modified their sampling design over a few years to reduce the variation around parr population estimates without success. Consequently, in 1998 snorkeling to obtain parr population estimates was discontinued and only General Parr Monitoring (GPM) sites are snorkeled. The number of redds observed in SBT-ISS streams has continued to decline as determined by five year cycles. Relatively weak strongholds continue to occur in the South Fork Salmon River and Bear Valley Creek. A rotary screw trap was operated on the West Fork Yankee Fork during the spring and fall of 1999 and the spring of 2000 to monitor juvenile chinook migration. A screw trap was also operated on the East Fork of the Salmon River during the spring and fall from 1993 to 1997 and 1999 (fall only) to 2000. Significant supplementation treatments have occurred in the South Fork Salmon River (IDFG). The East Fork Salmon River received supplementation treatments yearly through 1995. There have been no treatments since 1995, and no significant future treatments from local broodstock are conceivable due to extremely poor escapement. The West Fork Yankee Fork received a single presmolt treatment in 1994. Similarly, no significant future treatments are planned for the WFYF due to extremely poor escapement. However, small scale experimental captive rearing and broodstock techniques are currently being tested with populations from the EFSR and WFYF. Captive rearing/broodstock techniques could potentially provide feedback for evaluation of supplementation. The other three SBT-ISS streams are control streams and do not receive hatchery treatments.« less

27 CFR 9.113 - North Fork of Long Island.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries of...

27 CFR 9.113 - North Fork of Long Island.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries of...

27 CFR 9.113 - North Fork of Long Island.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries of...

High quality-factor quartz tuning fork glass probe used in tapping mode atomic force microscopy for surface profile measurement

NASA Astrophysics Data System (ADS)

Chen, Yuan-Liu; Xu, Yanhao; Shimizu, Yuki; Matsukuma, Hiraku; Gao, Wei

2018-06-01

This paper presents a high quality-factor (Q-factor) quartz tuning fork (QTF) with a glass probe attached, used in frequency modulation tapping mode atomic force microscopy (AFM) for the surface profile metrology of micro and nanostructures. Unlike conventionally used QTFs, which have tungsten or platinum probes for tapping mode AFM, and suffer from a low Q-factor influenced by the relatively large mass of the probe, the glass probe, which has a lower density, increases the Q-factor of the QTF probe unit allowing it to obtain better measurement sensitivity. In addition, the process of attaching the probe to the QTF with epoxy resin, which is necessary for tapping mode AFM, is also optimized to further improve the Q-factor of the QTF glass probe. The Q-factor of the optimized QTF glass probe unit is demonstrated to be very close to that of a bare QTF without a probe attached. To verify the effectiveness and the advantages of the optimized QTF glass probe unit, the probe unit is integrated into a home-built tapping mode AFM for conducting surface profile measurements of micro and nanostructures. A blazed grating with fine tool marks of 100 nm, a microprism sheet with a vertical amplitude of 25 µm and a Fresnel lens with a steep slope of 90 degrees are used as measurement specimens. From the measurement results, it is demonstrated that the optimized QTF glass probe unit can achieve higher sensitivity as well as better stability than conventional probes in the measurement of micro and nanostructures.

Mouse embryonic stem cells have increased capacity for replication fork restart driven by the specific Filia-Floped protein complex.

PubMed

Zhao, Bo; Zhang, Weidao; Cun, Yixian; Li, Jingzheng; Liu, Yan; Gao, Jing; Zhu, Hongwen; Zhou, Hu; Zhang, Rugang; Zheng, Ping

2018-01-01

Pluripotent stem cells (PSCs) harbor constitutive DNA replication stress during their rapid proliferation and the consequent genome instability hampers their applications in regenerative medicine. It is therefore important to understand the regulatory mechanisms of replication stress response in PSCs. Here, we report that mouse embryonic stem cells (ESCs) are superior to differentiated cells in resolving replication stress. Specifically, ESCs utilize a unique Filia-Floped protein complex-dependent mechanism to efficiently promote the restart of stalled replication forks, therefore maintaining genomic stability. The ESC-specific Filia-Floped complex resides on replication forks under normal conditions. Replication stress stimulates their recruitment to stalling forks and the serine 151 residue of Filia is phosphorylated in an ATR-dependent manner. This modification enables the Filia-Floped complex to act as a functional scaffold, which then promotes the stalling fork restart through a dual mechanism: both enhancing recruitment of the replication fork restart protein, Blm, and stimulating ATR kinase activation. In the Blm pathway, the scaffolds recruit the E3 ubiquitin ligase, Trim25, to the stalled replication forks, and in turn Trim25 tethers and concentrates Blm at stalled replication forks through ubiquitination. In differentiated cells, the recruitment of the Trim25-Blm complex to replication forks and the activation of ATR signaling are much less robust due to lack of the ESC-specific Filia-Floped scaffold. Thus, our study reveals that ESCs utilize an additional and unique regulatory layer to efficiently promote the stalled fork restart and maintain genomic stability.

Effects of Transposable Elements on the Expression of the Forked Gene of Drosophila Melanogaster

PubMed Central

Hoover, K. K.; Chien, A. J.; Corces, V. G.

1993-01-01

The products of the forked gene are involved in the formation and/or maintenance of a temporary fibrillar structure within the developing bristle rudiment of Drosophila melanogaster. Mutations in the forked locus alter this structure and result in aberrant development of macrochaetae, microchaetae and trichomes. The locus has been characterized at the molecular level by walking, mutant characterization and transcript analysis. Expression of the six forked transcripts is temporally restricted to midlate pupal development. At this time, RNAs of 6.4, 5.6, 5.4, 2.5, 1.9 and 1.1 kilobases (kb) are detected by Northern analysis. The coding region of these RNAs has been found to be within a 21-kb stretch of genomic DNA. The amino terminus of the proteins encoded by the 5.4- and 5.6-kb forked transcripts contain tandem copies of ankyrin-like repeats that may play an important role in the function of forked-encoded products. The profile of forked RNA expression is altered in seven spontaneous mutations characterized during this study. Three forked mutations induced by the insertion of the gypsy retrotransposon contain a copy of this element inserted into an intron of the gene. In these mutants, the 5.6-, 5.4- and 2.5-kb forked mRNAs are truncated via recognition of the polyadenylation site in the 5' long terminal repeat of the gypsy retrotransposon. These results help explain the role of the forked gene in fly development and further our understanding of the role of transposable elements in mutagenesis. PMID:8244011

Development of Tuning Fork Based Probes for Atomic Force Microscopy

NASA Astrophysics Data System (ADS)

Jalilian, Romaneh; Yazdanpanah, Mehdi M.; Torrez, Neil; Alizadeh, Amirali; Askari, Davood

2014-03-01

This article reports on the development of tuning fork-based AFM/STM probes in NaugaNeedles LLC for use in atomic force microscopy. These probes can be mounted on different carriers per customers' request. (e.g., RHK carrier, Omicron carrier, and tuning fork on a Sapphire disk). We are able to design and engineer tuning forks on any type of carrier used in the market. We can attach three types of tips on the edge of a tuning fork prong (i.e., growing Ag2Ga nanoneedles at any arbitrary angle, cantilever of AFM tip, and tungsten wire) with lengths from 100-500 μm. The nanoneedle is located vertical to the fork. Using a suitable insulation and metallic coating, we can make QPlus sensors that can detect tunneling current during the AFM scan. To make Qplus sensors, the entire quartz fork will be coated with an insulating material, before attaching the nanoneedle. Then, the top edge of one prong is coated with a thin layer of conductive metal and the nanoneedle is attached to the fork end of the metal coated prong. The metal coating provides electrical connection to the tip for tunneling current readout and to the electrodes and used to read the QPlus current. Since the amount of mass added to the fork is minimal, the resonance frequency spectrum does not change and still remains around 32.6 KHz and the Q factor is around 1,200 in ambient condition. These probes can enhance the performance of tuning fork based atomic microscopy.

Water quality of some logged and unlogged California streams

Treesearch

Fredric R. Kopperdahl; James W. Burns; Gary E. Smith

1971-01-01

Water quality was monitored in 1968 and 1969 in six coastal streams in northern California, four of which were subjected to logging and/or road building (Bummer Lake Creek, South Fork Yager Creek, Little North Fork Noyo River, and South Fork Caspar Creek), while the others remained undisturbed (Godwood Creek and North Fork Caspar Creek). The purposes of this study were...

Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery

PubMed Central

Murphy, Anar K.; Fitzgerald, Michael; Ro, Teresa; Kim, Jee Hyun; Rabinowitsch, Ariana I.; Chowdhury, Dipanjan; Schildkraut, Carl L.

2014-01-01

Phosphorylation of replication protein A (RPA) by Cdk2 and the checkpoint kinase ATR (ATM and Rad3 related) during replication fork stalling stabilizes the replisome, but how these modifications safeguard the fork is not understood. To address this question, we used single-molecule fiber analysis in cells expressing a phosphorylation-defective RPA2 subunit or lacking phosphatase activity toward RPA2. Deregulation of RPA phosphorylation reduced synthesis at forks both during replication stress and recovery from stress. The ability of phosphorylated RPA to stimulate fork recovery is mediated through the PALB2 tumor suppressor protein. RPA phosphorylation increased localization of PALB2 and BRCA2 to RPA-bound nuclear foci in cells experiencing replication stress. Phosphorylated RPA also stimulated recruitment of PALB2 to single-strand deoxyribonucleic acid (DNA) in a cell-free system. Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. These data demonstrate that phosphorylated RPA recruits repair factors to stalled forks, thereby enhancing fork integrity during replication stress. PMID:25113031

Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery.

PubMed

Murphy, Anar K; Fitzgerald, Michael; Ro, Teresa; Kim, Jee Hyun; Rabinowitsch, Ariana I; Chowdhury, Dipanjan; Schildkraut, Carl L; Borowiec, James A

2014-08-18

Phosphorylation of replication protein A (RPA) by Cdk2 and the checkpoint kinase ATR (ATM and Rad3 related) during replication fork stalling stabilizes the replisome, but how these modifications safeguard the fork is not understood. To address this question, we used single-molecule fiber analysis in cells expressing a phosphorylation-defective RPA2 subunit or lacking phosphatase activity toward RPA2. Deregulation of RPA phosphorylation reduced synthesis at forks both during replication stress and recovery from stress. The ability of phosphorylated RPA to stimulate fork recovery is mediated through the PALB2 tumor suppressor protein. RPA phosphorylation increased localization of PALB2 and BRCA2 to RPA-bound nuclear foci in cells experiencing replication stress. Phosphorylated RPA also stimulated recruitment of PALB2 to single-strand deoxyribonucleic acid (DNA) in a cell-free system. Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. These data demonstrate that phosphorylated RPA recruits repair factors to stalled forks, thereby enhancing fork integrity during replication stress. © 2014 Murphy et al.

Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.

PubMed

Reynolds, John J; Bicknell, Louise S; Carroll, Paula; Higgs, Martin R; Shaheen, Ranad; Murray, Jennie E; Papadopoulos, Dimitrios K; Leitch, Andrea; Murina, Olga; Tarnauskaitė, Žygimantė; Wessel, Sarah R; Zlatanou, Anastasia; Vernet, Audrey; von Kriegsheim, Alex; Mottram, Rachel M A; Logan, Clare V; Bye, Hannah; Li, Yun; Brean, Alexander; Maddirevula, Sateesh; Challis, Rachel C; Skouloudaki, Kassiani; Almoisheer, Agaadir; Alsaif, Hessa S; Amar, Ariella; Prescott, Natalie J; Bober, Michael B; Duker, Angela; Faqeih, Eissa; Seidahmed, Mohammed Zain; Al Tala, Saeed; Alswaid, Abdulrahman; Ahmed, Saleem; Al-Aama, Jumana Yousuf; Altmüller, Janine; Al Balwi, Mohammed; Brady, Angela F; Chessa, Luciana; Cox, Helen; Fischetto, Rita; Heller, Raoul; Henderson, Bertram D; Hobson, Emma; Nürnberg, Peter; Percin, E Ferda; Peron, Angela; Spaccini, Luigina; Quigley, Alan J; Thakur, Seema; Wise, Carol A; Yoon, Grace; Alnemer, Maha; Tomancak, Pavel; Yigit, Gökhan; Taylor, A Malcolm R; Reijns, Martin A M; Simpson, Michael A; Cortez, David; Alkuraya, Fowzan S; Mathew, Christopher G; Jackson, Andrew P; Stewart, Grant S

2017-04-01

To ensure efficient genome duplication, cells have evolved numerous factors that promote unperturbed DNA replication and protect, repair and restart damaged forks. Here we identify downstream neighbor of SON (DONSON) as a novel fork protection factor and report biallelic DONSON mutations in 29 individuals with microcephalic dwarfism. We demonstrate that DONSON is a replisome component that stabilizes forks during genome replication. Loss of DONSON leads to severe replication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks. Furthermore, ATM- and Rad3-related (ATR)-dependent signaling in response to replication stress is impaired in DONSON-deficient cells, resulting in decreased checkpoint activity and the potentiation of chromosomal instability. Hypomorphic mutations in DONSON substantially reduce DONSON protein levels and impair fork stability in cells from patients, consistent with defective DNA replication underlying the disease phenotype. In summary, we have identified mutations in DONSON as a common cause of microcephalic dwarfism and established DONSON as a critical replication fork protein required for mammalian DNA replication and genome stability.

Checkpoint-dependent RNR induction promotes fork restart after replicative stress.

PubMed

Morafraile, Esther C; Diffley, John F X; Tercero, José Antonio; Segurado, Mónica

2015-01-20

The checkpoint kinase Rad53 is crucial to regulate DNA replication in the presence of replicative stress. Under conditions that interfere with the progression of replication forks, Rad53 prevents Exo1-dependent fork degradation. However, although EXO1 deletion avoids fork degradation in rad53 mutants, it does not suppress their sensitivity to the ribonucleotide reductase (RNR) inhibitor hydroxyurea (HU). In this case, the inability to restart stalled forks is likely to account for the lethality of rad53 mutant cells after replication blocks. Here we show that Rad53 regulates replication restart through the checkpoint-dependent transcriptional response, and more specifically, through RNR induction. Thus, in addition to preventing fork degradation, Rad53 prevents cell death in the presence of HU by regulating RNR-expression and localization. When RNR is induced in the absence of Exo1 and RNR negative regulators, cell viability of rad53 mutants treated with HU is increased and the ability of replication forks to restart after replicative stress is restored.

Eukaryotic DNA Replication Fork.

PubMed

Burgers, Peter M J; Kunkel, Thomas A

2017-06-20

This review focuses on the biogenesis and composition of the eukaryotic DNA replication fork, with an emphasis on the enzymes that synthesize DNA and repair discontinuities on the lagging strand of the replication fork. Physical and genetic methodologies aimed at understanding these processes are discussed. The preponderance of evidence supports a model in which DNA polymerase ε (Pol ε) carries out the bulk of leading strand DNA synthesis at an undisturbed replication fork. DNA polymerases α and δ carry out the initiation of Okazaki fragment synthesis and its elongation and maturation, respectively. This review also discusses alternative proposals, including cellular processes during which alternative forks may be utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leading and lagging strand DNA synthesis separately and as an integrated replication fork.

Operations Plans for Anadromous Fish Production Facilities in the Columbia River Basin, Volume II of V; 1992 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hutchison, Bill

1993-05-01

Clearwater Hatchery is located on the north bank of the North Fork of the Clearwater River, downstream from Dworshak Dam. It is approximately 72 miles from Lower Granite Dam, and 504 miles from the mouth of the Columbia River. Site elevation is approximately 994 feet above sea level. The hatchery is staffed with 7 FTE's. Clearwater Hatchery has two pipelines from Dworshak Reservoir. One is attached to a floating platform and is capable of providing various temperatures at varying depths. The other is a stationary intake about 245 feet below the top of the dam. All water is gravity fedmore » to the hatchery. An l8 inch intake pipe provides an estimated 10 cfs with temperature remaining constant at approximately 40 F. The primary 42-inch intake pipe can draw water from 5 to 45 feet in depth with temperatures ranging from 55 to 60 F and 70 cfs of flow. The hatchery facility consists of 11 chinook raceways, 24 steelhead raceways, 2 adult holding ponds, a covered spawning area with 2 live wells and 60 concrete rearing vats. There are 40 double stacks of Heath-type incubators and each vat also has an incubation jar. All facility units are in excellent condition. Clearwater Hatchery also supports satellite facilities at Red River, Crooked River and Powell. The Red River satellite facility is located approximately 15 miles east of Elk City, Idaho. It is approximately 186 miles upstream from Lower Granite Dam and 618 miles from the mouth of the Columbia River. It was first built in 1974 by the Columbia River Project and then remodeled by the U.S. Army Corps of Engineers in 1986. Red River is supplied by gravity flow from an intake located at the bottom of the South Fork of Red River, 225 yards upstream from the facility. Water rights allow for 10 cfs and during low flows in the summer about 5 cfs is available. Temperatures range from 40 F in the spring to 71 F in early August. The facility consists of two adult holding ponds, a removable tripod and panel weir, and a rearing pond. All units are in good condition due to the recent remodeling. The Crooked River satellite facility is located 20 miles downstream of Red River. The trap is located 0.5 miles upstream of the mouth of Crooked River, a tributary of the South Fork of the Clearwater River. The rearing ponds are 10 miles upstream from the Crooked River adult trap. Crooked River water is supplied by gravity flow by an intake 200 yards upstream of the facility raceways. Water rights allow for 10 cfs at the rearing facility and 10 cfs at the trapping facility. Water temperatures range from 42 to 70 F. The trap and weir are located at the mouth of Crooked River. Ten miles upstream from the mouth are two raceways, a cleaning waste pond and final settling pond. All facility units are in good condition. The Powell satellite facility is located 122 miles east of the Clear-water Hatchery at the headwaters of the Lochsa River, the confluence of the Crooked Fork Creek and White Sands Creek. Powell is 192.5 miles from Lower Granite Dam and 624 miles from the mouth of the Columbia River. The Powell Facility receives gravity flow water from Walton Creek at a rate of 7 cfs with the intake being located 100 yards upstream from the facility. Powell also has a pumped supply from White Sands Creek at 3 cfs. Water temperature ranges from 45.8 to 50.2 F from the Walton Creek intake and 41 to 65 F from the White Sands pump station. The facility consists of one rearing pond, a diversion and intake screen, two adult holding ponds, a floating weir, and an open bay spawning shelter. All facility units are in good condition.« less

Salmon Supplementation Studies in Idaho Rivers, 1996-1998 Progress Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reighn, Christopher A.; Lewis, Bert; Taki, Doug

1999-06-01

Information contained in this report summarizes the work that has been done by the Shoshone-Bannock Tribes Fisheries Department under BPA Project No. 89-098-3, Contract Number 92-BI-49450. Relevant data generated by the Shoshone-Bannock Tribe will be collated with other ISS cooperator data collected from the Salmon and Clearwater rivers and tributary streams. A summary of data presented in this report and an initial project-wide level supplementation evaluation will be available in the ISS 5 year report that is currently in progress. The Shoshone-Bannock Tribal Fisheries Department is responsible for monitoring a variety of chinook salmon (Oncorhynchus tshawytscha) production parameters as partmore » of the Idaho Supplementation Studies (BPA Project No. 89-098-3, Contract Number 92-BI-49450). Parameters include parr abundance in tributaries to the upper Salmon River; adult chinook salmon spawner abundance, redd counts, and carcass collection. A rotary screw trap is operated on the East Fork Salmon River and West Fork Yankee Fork Salmon River to enumerate and PIT-tag chinook smolts. These traps are also used to monitor parr movement, and collect individuals for the State and Tribal chinook salmon captive rearing program. The SBT monitors fisheries parameters in the following six tributaries of the Salmon River: Bear Valley Creek, East Fork Salmon River, Herd Creek, South Fork Salmon River, Valley Creek, and West Fork Yankee Fork. Chinook populations in all SBT-ISS monitored streams continue to decline. The South Fork Salmon River and Bear Valley Creek have the strongest remaining populations. Snorkel survey methodology was used to obtain parr population estimates for ISS streams from 1992 to 1997. Confidence intervals for the parr population estimates were large, especially when the populations were low. In 1998, based on ISS cooperator agreement, snorkeling to obtain parr population estimates was ceased due to the large confidence intervals. A rotary screw trap was operated on the West Fork Yankee Fork during the spring, summer, and fall of 1998 to monitor juvenile chinook migration. A screw trap was also operated on the East Fork of the Salmon River during the spring and fall from 1993 to 1997. Supplementation treatments have occurred on the South Fork Salmon River (IDFG), the East Fork Salmon River (EFSR), and the West Fork Yankee Fork of the Salmon River (WFYF). The EFSR received supplementation treatments yearly through 1995. There have been no treatments since 1995, and no significant future treatments from local broodstock are planned due to extremely poor escapement. The WFYF received a single presmolt treatment in 1994. There was an egg and adult release treatment in 1998 from the captive rearing program, not part of the original ISS study. Similarly, no significant future treatments are planned for the West Fork Yankee Fork due to extremely poor escapement. However, small scale experimental captive rearing and broodstock techniques are currently being tested with populations from the EFSR and WFYF. Captive rearing/broodstock techniques could potentially provide feedback for evaluation of supplementation. The other three SBT-ISS streams are control streams and do not receive supplementation treatments.« less

Hydraulic geometry and sediment data for the South Fork Salmon River, Idaho, 1985-86

USGS Publications Warehouse

Williams, Rhea P.; O'Dell, Ivalou; Megahan, Walter F.

1989-01-01

Hydraulic geometry, suspended-sediment, and bedload samples were collected at three sites in the upper reach of the South Fork Salmon River drainage basin from April 1985 to June 1986. Sites selected were South Fork Salmon River near Krassel Ranger Station, Buckhorn Creek, and North Fork Lick Creek. Results of the data collection are presented in this report.

75 FR 60740 - Record of Decision for the Base Closure and Realignment Beddown and Flight Operations of Remotely...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-01

... DEPARTMENT OF DEFENSE Department of the Air Force Record of Decision for the Base Closure and Realignment Beddown and Flight Operations of Remotely Piloted Aircraft at Grand Forks Air Force Base (AFB), ND... United States Air Force signed the ROD for the 2005 Base Closure and Realignment (BRAC 2005) directed...

1. Roaring Fork Motor Nature Trail, entrance sign. Great ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

9. Roaring Fork Motor Nature Trail, Reagan House. Great ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

Regulation of Replication Fork Advance and Stability by Nucleosome Assembly

PubMed Central

Prado, Felix; Maya, Douglas

2017-01-01

The advance of replication forks to duplicate chromosomes in dividing cells requires the disassembly of nucleosomes ahead of the fork and the rapid assembly of parental and de novo histones at the newly synthesized strands behind the fork. Replication-coupled chromatin assembly provides a unique opportunity to regulate fork advance and stability. Through post-translational histone modifications and tightly regulated physical and genetic interactions between chromatin assembly factors and replisome components, chromatin assembly: (1) controls the rate of DNA synthesis and adjusts it to histone availability; (2) provides a mechanism to protect the integrity of the advancing fork; and (3) regulates the mechanisms of DNA damage tolerance in response to replication-blocking lesions. Uncoupling DNA synthesis from nucleosome assembly has deleterious effects on genome integrity and cell cycle progression and is linked to genetic diseases, cancer, and aging. PMID:28125036

Channelization and floodplain forests: impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

Treesearch

Sonja N. Oswalt; Sammy L. King

2005-01-01

We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased...

At the centre of the tuning fork

NASA Image and Video Library

2015-11-02

This galaxy is known as Mrk 820 and is classified as a lenticular galaxy — type S0 on the Hubble Tuning Fork. The Hubble Tuning Fork is used to classify galaxies according to their morphology. Elliptical galaxies look like smooth blobs in the sky and lie on the handle of the fork. They are arranged along the handle based on how elliptical they are, with the more spherical galaxies furthest from the tines of the fork, and the more egg-shaped ones closest to the end of the handle where it divides. The two prongs of the tuning fork represent types of unbarred and barred spiral galaxies. Lenticular galaxies like Mrk 820 are in the transition zone between ellipticals and spirals and lie right where the fork divides. A closer look at the appearance of Mrk 820 reveals hints of a spiral structure embedded in a circular halo of stars. Surrounding Mrk 820 in this image is good sampling of other galaxy types, covering almost every type found on the Hubble Tuning Fork, both elliptical and spiral. Most of the smears and specks are distant galaxies, but the prominent bright object at the bottom is a foreground star called TYC 4386-787-1. A version of this image was entered into the Hubble's Hidden Treasures image processing competition by contestant Judy Schmidt.

Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments.

PubMed

Kolinjivadi, Arun Mouli; Sannino, Vincenzo; De Antoni, Anna; Zadorozhny, Karina; Kilkenny, Mairi; Técher, Hervé; Baldi, Giorgio; Shen, Rong; Ciccia, Alberto; Pellegrini, Luca; Krejci, Lumir; Costanzo, Vincenzo

2017-09-07

Brca2 deficiency causes Mre11-dependent degradation of nascent DNA at stalled forks, leading to cell lethality. To understand the molecular mechanisms underlying this process, we isolated Xenopus laevis Brca2. We demonstrated that Brca2 protein prevents single-stranded DNA gap accumulation at replication fork junctions and behind them by promoting Rad51 binding to replicating DNA. Without Brca2, forks with persistent gaps are converted by Smarcal1 into reversed forks, triggering extensive Mre11-dependent nascent DNA degradation. Stable Rad51 nucleofilaments, but not RPA or Rad51 T131P mutant proteins, directly prevent Mre11-dependent DNA degradation. Mre11 inhibition instead promotes reversed fork accumulation in the absence of Brca2. Rad51 directly interacts with the Pol α N-terminal domain, promoting Pol α and δ binding to stalled replication forks. This interaction likely promotes replication fork restart and gap avoidance. These results indicate that Brca2 and Rad51 prevent formation of abnormal DNA replication intermediates, whose processing by Smarcal1 and Mre11 predisposes to genome instability. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

Co-localization of polar replication fork barriers and rRNA transcription terminators in mouse rDNA.

PubMed

López-estraño, C; Schvartzman, J B; Krimer, D B; Hernández, P

1998-03-27

We investigated the replication of the region where transcription terminates in mouse rDNA. It contains a replication fork barrier (RFB) that behaves in a polar manner, arresting only replication forks moving in the direction opposite to transcription. This RFB consists of several closely spaced fork arrest sites that co-localize with the transcription terminator elements, known as Sal boxes. Sal boxes are the target for mTTF-I (murine transcription termination factor I). These results suggest that both termination of rRNA transcription and replication fork arrest may share cis-acting as well as trans-acting factors. Copyright 1998 Academic Press Limited.

On the sound field radiated by a tuning fork

NASA Astrophysics Data System (ADS)

Russell, Daniel A.

2000-12-01

When a sounding tuning fork is brought close to the ear, and rotated about its long axis, four distinct maxima and minima are heard. However, when the same tuning fork is rotated while being held at arm's length from the ear only two maxima and minima are heard. Misconceptions concerning this phenomenon are addressed and the fundamental mode of the fork is described in terms of a linear quadrupole source. Measured directivity patterns in the near field and far field of several forks agree very well with theoretical predictions for a linear quadrupole. Other modes of vibration are shown to radiate as dipole and lateral quadrupole sources.

8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

ATR-like kinase Mec1 facilitates both chromatin accessibility at DNA replication forks and replication fork progression during replication stress

PubMed Central

Rodriguez, Jairo; Tsukiyama, Toshio

2013-01-01

Faithful DNA replication is essential for normal cell division and differentiation. In eukaryotic cells, DNA replication takes place on chromatin. This poses the critical question as to how DNA replication can progress through chromatin, which is inhibitory to all DNA-dependent processes. Here, we developed a novel genome-wide method to measure chromatin accessibility to micrococcal nuclease (MNase) that is normalized for nucleosome density, the NCAM (normalized chromatin accessibility to MNase) assay. This method enabled us to discover that chromatin accessibility increases specifically at and ahead of DNA replication forks in normal S phase and during replication stress. We further found that Mec1, a key regulatory ATR-like kinase in the S-phase checkpoint, is required for both normal chromatin accessibility around replication forks and replication fork rate during replication stress, revealing novel functions for the kinase in replication stress response. These results suggest a possibility that Mec1 may facilitate DNA replication fork progression during replication stress by increasing chromatin accessibility around replication forks. PMID:23307868

Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities.

PubMed

Ward, T R; Hoang, M L; Prusty, R; Lau, C K; Keil, R L; Fangman, W L; Brewer, B J

2000-07-01

In the ribosomal DNA of Saccharomyces cerevisiae, sequences in the nontranscribed spacer 3' of the 35S ribosomal RNA gene are important to the polar arrest of replication forks at a site called the replication fork barrier (RFB) and also to the cis-acting, mitotic hyperrecombination site called HOT1. We have found that the RFB and HOT1 activity share some but not all of their essential sequences. Many of the mutations that reduce HOT1 recombination also decrease or eliminate fork arrest at one of two closely spaced RFB sites, RFB1 and RFB2. A simple model for the juxtaposition of RFB and HOT1 sequences is that the breakage of strands in replication forks arrested at RFB stimulates recombination. Contrary to this model, we show here that HOT1-stimulated recombination does not require the arrest of forks at the RFB. Therefore, while HOT1 activity is independent of replication fork arrest, HOT1 and RFB require some common sequences, suggesting the existence of a common trans-acting factor(s).

Man-induced channel adjustment in Tennessee streams

USGS Publications Warehouse

Robbins, C.H.; Simon, Andrew

1983-01-01

Channel modifications in Tennessee, particularly in the western part, have led to large-scale instabilities in the channelized rivers and may have contributed to several bridge failures. These modifications, together with land-use practices, led to downcutting, headward erosion, downstream aggradation, accelerated scour, and bank instabilities. Changes in gradient by channel straightening caused more severe channel response than did dredging or clearing. Large-scale changes continue to occur in all the channelized rivers: the Obion River, its forks, and the South Fork Forked Deer River. However, the non-channelized Hatchie River in west Tennessee not only withstood the natural stresses imposed by the wet years of 1973 to 1975 but continues to exhibit characteristics of stability. Water-surface slope, the primary dependent variable, proved to be a sensitive and descriptive parameter useful in determining channel adjustment. Adjustments to man-induced increases in channel-slope are described by inverse exponential functions of the basic form S=ae(-b(t)); where ' S ' is some function describing channel-slope, ' t ' is the number of years since completion of channel work, and ' a ' and ' b ' are coefficients. Response times for the attainment of ' equilibrium ' channel slopes are a function of the magnitude and extent of the imposed modifications. The adjusted profile gradients attained by the streams following channelization are similar to the predisturbed profile gradients, where no alteration to channel length was made. Where the channels were straightened by constructing cut-offs, thus shortening channel length, then slope adjustments (reduction) proceed past the predisturbed profile gradients, to new profiles with lower gradients. (USGS)

LOOKING EASTSOUTHEAST. Showing downstream side of completed bridge, from confluence ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOOKING EAST-SOUTHEAST. Showing downstream side of completed bridge, from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

7. Roaring Fork Motor Nature Trail, rocks along edge of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

5. Roaring Fork Motor Nature Trail, vista at stop three. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO SETTLING BASIN, SHOWING RIGHT FORK WITH GATE IN PLACE AND A FEW NEEDLES IN PLACE - Electron Hydroelectric Project, Along Puyallup River, Electron, Pierce County, WA

2. Roaring Fork Motor Nature Trail, road view before first ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

6. Roaring Fork Motor Nature Trail, road view after stop ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

5. VIEW EAST ACROSS BRIDGE SHOWING RAILING DETAILS AND WATERFALLS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. VIEW EAST ACROSS BRIDGE SHOWING RAILING DETAILS AND WATERFALLS OF THE SOUTH FORK OF THE TUOLUMNE. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA

Evaluating the impacts of logging activities on erosion and suspended sediment transport in the Caspar Creek watersheds

Treesearch

Jack Lewis

1998-01-01

Suspended sediment has been sampled at both the North and South Fork weirs of Caspar Creek in northwestern California since 1963, and at 13 tributary locations in the North Fork since 1986. The North Fork gaging station (NFC) was used as a control to evaluate the effects of logging in the South Fork, in the 1970's, on annual sediment loads. In the most...

South Fork Latrine, east elevation showing structure in context, view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Latrine, east elevation showing structure in context, view west - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

3. Roaring Fork Motor Nature Trail, view between second and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

11. Roaring Fork Motor Nature Trail, boulders along road after ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

2. VIEW OF NORTH FACE SHOWING SUBSTRUCTURE AND ABUTMENTS OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. VIEW OF NORTH FACE SHOWING SUBSTRUCTURE AND ABUTMENTS OF BRIDGE CROSSING THE SOUTH FORK OF THE TUOLUMNE RIVER. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA

Distribution and movement of bull trout in the upper Jarbidge River watershed, Nevada

USGS Publications Warehouse

Allen, M. Brady; Connolly, Patrick J.; Mesa, Matthew G.; Charrier, Jodi; Dixon, Chris

2010-01-01

In 2006 and 2007, we surveyed the occurrence of bull trout (Salvelinus confluentus), the relative distributions of bull trout and redband trout (Oncorhynchus mykiss), and stream habitat conditions in the East and West Forks of the Jarbidge River in northeastern Nevada and southern Idaho. We installed passive integrated transponder (PIT) tag interrogation systems at strategic locations within the watershed, and PIT-tagged bull trout were monitored to evaluate individual fish growth, movement, and the connectivity of bull trout between streams. Robust bull trout populations were found in the upper portions of the East Fork Jarbidge River, the West Fork Jarbidge River, and in the Pine, Jack, Dave, and Fall Creeks. Small numbers of bull trout also were found in Slide and Cougar Creeks. Bull trout were numerically dominant in the upper portions of the East Fork Jarbidge River, and in Fall, Dave, Jack, and Pine Creeks, whereas redband trout were numerically dominant throughout the rest of the watershed. The relative abundance of bull trout was notably higher at altitudes above 2,100 m. This study was successful in documenting bull trout population connectivity within the West Fork Jarbidge River, particularly between West Fork Jarbidge River and Pine Creek. Downstream movement of bull trout to the confluence of the East Fork and West Fork Jarbidge River both from Jack Creek (rkm 16.6) in the West Fork Jarbidge River and from Dave Creek (rkm 7.5) in the East Fork Jarbidge River was detected. Although bull trout exhibited some downstream movement during the spring and summer, much of their emigration occurred in the autumn, concurrent with decreasing water temperatures and slightly increasing flows. The bull trout that emigrated were mostly age-2 or older, but some age-1 fish also emigrated. Upstream movement by bull trout was detected less than downstream movement. The overall mean annual growth rate of bull trout in the East Fork and West Fork Jarbidge River was 36 mm. This growth rate is within the range reported in other river systems and is indicative of good habitat conditions. Mark-recapture methods were used to estimate a population of 147 age-1 or older bull trout in the reach of Jack Creek upstream of Jenny Creek.

Electromagnetic analysis of groundwater on the Arizona-Utah border

NASA Astrophysics Data System (ADS)

Vander Vis, T.; Porter, R. C.; Macy, J. P.

2016-12-01

Understanding subsurface structure and groundwater flow is an essential part of managing groundwater resources, especially in southwestern United States where supply is limited and demand is increasing. This study describes the preliminary results of a transient electromagnetic survey conducted on the Arizona-Utah border to better understand the groundwater system which supplies water to many wells and springs in the region. Electromagnetic surveys are ideal for groundwater investigations because they can locate and characterize areas of high conductivity, which often are indicative of groundwater. The study area is on the southwestern margin of the Colorado Plateau and consists of uplifted, flat-lying sedimentary units. Regionally, groundwater is located within the Navajo Sandstone and underlying Kayenta Formation as an unconfined aquifer that extends from Pipe Springs National Monument north to the East Fork of the Virgin River. This area is characterized by step-like structural blocks that accommodate small amounts of extension and are bounded by long north-south-trending normal faults. The Sevier Fault runs through the sedimentary units near the study area and has been shown to influence groundwater movement by offsetting permeable units west of the fault adjacent to impermeable units east of the fault. Electromagnetic measurements were recorded with a Zonge GDP-32 receiver at 30 receiver locations at 16 and 32 Hz with a 100mx100m transmitter loop. These data were used to create a subsurface conductivity model. Water levels from local wells and local geologic data were utilized to relate the geophysical data to the groundwater system. Preliminary results define the depth to water table and the location of the groundwater divide between the groundwater that flows north towards the springs that feed the East Fork of the Virgin River and the groundwater that flows south towards Pipe Springs National Monument.

A Cultural Resource Reconnaissance of Two Proposed Bank Unloading Areas, East Grand Forks, Polk County, Minnesota,

DTIC Science & Technology

1984-12-01

architectural or archeotogical evidence was identified. The southern unloading area includes the former sites of a brewery and a sawmill, both of... brewery and a sawmill, both of which were associated with 0 significant historic events and themes in the late nineteenth and early twentieth century...Forks Brewery (1888) . . . . . . . 16 Figure 4. The Grand Forks Lumber Company mill is located at A. -. Building at B is probably the East Grand Forks

A dual role of BRCA1 in two distinct homologous recombination mediated repair in response to replication arrest

PubMed Central

Feng, Zhihui; Zhang, Junran

2012-01-01

Homologous recombination (HR) is a major mechanism utilized to repair blockage of DNA replication forks. Here, we report that a sister chromatid exchange (SCE) generated by crossover-associated HR efficiently occurs in response to replication fork stalling before any measurable DNA double-strand breaks (DSBs). Interestingly, SCE produced by replication fork collapse following DNA DSBs creation is specifically suppressed by ATR, a central regulator of the replication checkpoint. BRCA1 depletion leads to decreased RPA2 phosphorylation (RPA2-P) following replication fork stalling but has no obvious effect on RPA2-P following replication fork collapse. Importantly, we found that BRCA1 promotes RAD51 recruitment and SCE induced by replication fork stalling independent of ATR. In contrast, BRCA1 depletion leads to a more profound defect in RAD51 recruitment and SCE induced by replication fork collapse when ATR is depleted. We concluded that BRCA1 plays a dual role in two distinct HR-mediated repair upon replication fork stalling and collapse. Our data established a molecular basis for the observation that defective BRCA1 leads to a high sensitivity to agents that cause replication blocks without being associated with DSBs, and also implicate a novel mechanism by which loss of cell cycle checkpoints promotes BRCA1-associated tumorigenesis via enhancing HR defect resulting from BRCA1 deficiency. PMID:21954437

Dissolved-Solids Load in Henrys Fork Upstream from the Confluence with Antelope Wash, Wyoming, Water Years 1970-2009

USGS Publications Warehouse

Foster, Katharine; Kenney, Terry A.

2010-01-01

Annual dissolved-solids load at the mouth of Henrys Fork was estimated by using data from U.S. Geological Survey streamflow-gaging station 09229500, Henrys Fork near Manila, Utah. The annual dissolved-solids load for water years 1970-2009 ranged from 18,300 tons in 1977 to 123,300 tons in 1983. Annual streamflows for this period ranged from 14,100 acre-feet in 1977 to 197,500 acre-feet in 1983. The 25-percent trimmed mean dissolved-solids load for water years 1970-2009 was 44,300 tons per year at Henrys Fork near Manila, Utah. Previous simulations using a SPAtially Referenced Regression On Watershed attributes (SPARROW) model for dissolved solids specific to water year 1991 conditions in the Upper Colorado River Basin predicted an annual dissolved-solids load of 25,000 tons for the Henrys Fork Basin upstream from Antelope Wash. On the basis of computed dissolved-solids load data from Henrys Fork near Manila, Utah, together with estimated annual dissolved-solids load from Antelope Wash and Peoples Canal, this prediction was adjusted to 37,200 tons. As determined by simulations with the Upper Colorado River Basin SPARROW model, approximately 56 percent (14,000 tons per year) of the dissolved-solids load at Henrys Fork upstream from Antelope Wash is associated with the 21,500 acres of irrigated agricultural lands in the upper Henrys Fork Basin.

Estimating canopy fuel characteristics in five conifer stands in the western United States using tree and stand measurements

Treesearch

Elizabeth Reinhardt; Joe Scott; Kathy Gray; Robert Keane

2006-01-01

Isolated wilderness ecosystems with a history of frequent, low-severity fires have been altered due to many decades of fire exclusion and, as a result, are difficult to restore for philosophical and logistical reasons. In this paper, we describe the successional conditions of ponderosa pine (Pinus ponderosa) communities along the South Fork of the...

South Fork Latrine, oblique view showing south and east sides; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Latrine, oblique view showing south and east sides; view northwest - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

South Fork Latrine showing north and west sides, general view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Latrine showing north and west sides, general view to southeast - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

COMPLETED STRUCTURE. View is eastsoutheast of downstream side of bridge, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

COMPLETED STRUCTURE. View is east-southeast of downstream side of bridge, from beyond confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

7. View to southeast. View of downstream side of bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. View to southeast. View of downstream side of bridge from confluence of Trinity and South Fork Trinity Rivers. (90mm Lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

LAKE FORK

EPA Science Inventory

The Lake Fork of the Arkansas River Watershed has been adversely affected through mining, water diversion and storage projects, grazing, logging, and other human influences over the past 120 years. It is the goals of the LFWWG to improve the health of Lake fork by addressing th...

12. Roaring Fork Motor Nature Trail, place of a thousand ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

Multistory duplexes with forward dipping roofs, north central Brooks Range, Alaska

USGS Publications Warehouse

Wallace, W.K.; Moore, Thomas E.; Plafker, G.

1997-01-01

The Endicott Mountains allochthon has been thrust far northward over the North Slope parautochthon in the northern Brooks Range. Progressively younger units are exposed northward within the allochthon. To the south, the incompetent Hunt Fork Shale has thickened internally by asymmetric folds and thrust faults. Northward, the competent Kanayut Conglomerate forms a duplex between a floor thrust in Hunt Fork and a roof thrust in the Kayak Shale. To the north, the competent Lisburne Group forms a duplex between a floor thrust in Kayak and a roof thrust in the Siksikpuk Formation. Both duplexes formed from north vergent detachment folds whose steep limbs were later truncated by south dipping thrust faults that only locally breach immediately overlying roof thrusts. Within the parautochthon, the Kayak, Lisburne, and Siksikpuk-equivalent Echooka Formation form a duplex identical to that in the allochthon. This duplex is succeeded abruptly northward by detachment folds in Lisburne. These folds are parasitic to an anticlinorium interpreted to reflect a fault-bend folded horse in North Slope "basement," with a roof thrust in Kayak and a floor thrust at depth. These structures constitute two northward tapered, internally deformed wedges that are juxtaposed at the base of the allochthon. Within each wedge, competent units have been shortened independently between detachments, located mainly in incompetent units. The basal detachment of each wedge cuts upsection forward (northward) to define a wedge geometry within which units dip regionally forward. These dips reflect forward decrease in internal structural thickening by forward vergent folds and hindward dipping thrust faults. Copyright 1997 by the American Geophysical Union.

Force regulated dynamics of RPA on a DNA fork

PubMed Central

Kemmerich, Felix E.; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

2016-01-01

Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg2+ concentrations, such that human RPA can melt DNA in absence of force. PMID:27016742

South Fork Telephone Switchboard Building, oblique view of east side; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Telephone Switchboard Building, oblique view of east side; view northwest - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

South Fork Telephone Switchboard Building, interior west room showing hardwood ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Telephone Switchboard Building, interior west room showing hardwood floor; view south - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

South Fork Latrine, interior showing head with steel tank mounted ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Latrine, interior showing head with steel tank mounted to wall; view south - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

14. Roaring Fork Motor Nature Trail, Place of a thousand ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

Class I Histone Deacetylase HDAC1 and WRN RECQ Helicase Contribute Additively to Protect Replication Forks upon Hydroxyurea-induced Arrest.

PubMed

Kehrli, Keffy; Phelps, Michael; Lazarchuk, Pavlo; Chen, Eleanor; Monnat, Ray; Sidorova, Julia M

2016-11-18

The WRN helicase/exonuclease is mutated in Werner syndrome of genomic instability and premature aging. WRN-depleted fibroblasts, although remaining largely viable, have a reduced capacity to maintain replication forks active during a transient hydroxyurea-induced arrest. A strand exchange protein, RAD51, is also required for replication fork maintenance, and here we show that recruitment of RAD51 to stalled forks is reduced in the absence of WRN. We performed a siRNA screen for genes that are required for viability of WRN-depleted cells after hydroxyurea treatment, and identified HDAC1, a member of the class I histone deacetylase family. One of the functions of HDAC1, which it performs together with a close homolog HDAC2, is deacetylation of new histone H4 deposited at replication forks. We show that HDAC1 depletion exacerbates defects in fork reactivation and progression after hydroxyurea treatment observed in WRN- or RAD51-deficient cells. The additive WRN, HDAC1 loss-of-function phenotype is also observed with a catalytic mutant of HDAC1; however, it does not correlate with changes in histone H4 deacetylation at replication forks. On the other hand, inhibition of histone deacetylation by an inhibitor specific to HDACs 1-3, CI-994, correlates with increased processing of newly synthesized DNA strands in hydroxyurea-stalled forks. WRN co-precipitates with HDAC1 and HDAC2. Taken together, our findings indicate that WRN interacts with HDACs 1 and 2 to facilitate activity of stalled replication forks under conditions of replication stress. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

South Fork Telephone Switchboard Building, general view in setting showing ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Telephone Switchboard Building, general view in setting showing (N) side; view (S) - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

South Fork Telephone Switchboard Building, oblique view of (W) and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

South Fork Telephone Switchboard Building, oblique view of (W) and (S) sides, view to northeast - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

STEEL ERECTION. View of downstream of bridge, looking southeast from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

STEEL ERECTION. View of downstream of bridge, looking southeast from confluence of Trinity and South Fork Trinity rivers. The old suspension bridge is in background - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe.

PubMed

Margalef, Pol; Kotsantis, Panagiotis; Borel, Valerie; Bellelli, Roberto; Panier, Stephanie; Boulton, Simon J

2018-01-25

Telomere maintenance critically depends on the distinct activities of telomerase, which adds telomeric repeats to solve the end replication problem, and RTEL1, which dismantles DNA secondary structures at telomeres to facilitate replisome progression. Here, we establish that reversed replication forks are a pathological substrate for telomerase and the source of telomere catastrophe in Rtel1 -/- cells. Inhibiting telomerase recruitment to telomeres, but not its activity, or blocking replication fork reversal through PARP1 inhibition or depleting UBC13 or ZRANB3 prevents the rapid accumulation of dysfunctional telomeres in RTEL1-deficient cells. In this context, we establish that telomerase binding to reversed replication forks inhibits telomere replication, which can be mimicked by preventing replication fork restart through depletion of RECQ1 or PARG. Our results lead us to propose that telomerase inappropriately binds to and inhibits restart of reversed replication forks within telomeres, which compromises replication and leads to critically short telomeres. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes.

PubMed

Hilton, Benjamin A; Liu, Ji; Cartwright, Brian M; Liu, Yiyong; Breitman, Maya; Wang, Youjie; Jones, Rowdy; Tang, Hui; Rusinol, Antonio; Musich, Phillip R; Zou, Yue

2017-09-01

Hutchinson-Gilford progeria syndrome (HGPS) is a rare genetic disorder that is caused by a point mutation in the LMNA gene, resulting in production of a truncated farnesylated-prelamin A protein (progerin). We previously reported that XPA mislocalized to the progerin-induced DNA double-strand break (DSB) sites, blocking DSB repair, which led to DSB accumulation, DNA damage responses, and early replication arrest in HGPS. In this study, the XPA mislocalization to DSBs occurred at stalled or collapsed replication forks, concurrent with a significant loss of PCNA at the forks, whereas PCNA efficiently bound to progerin. This PCNA sequestration likely exposed ds-ssDNA junctions at replication forks for XPA binding. Depletion of XPA or progerin each significantly restored PCNA at replication forks. Our results suggest that although PCNA is much more competitive than XPA in binding replication forks, PCNA sequestration by progerin may shift the equilibrium to favor XPA binding. Furthermore, we demonstrated that progerin-induced apoptosis could be rescued by XPA, suggesting that XPA-replication fork binding may prevent apoptosis in HGPS cells. Our results propose a mechanism for progerin-induced genome instability and accelerated replicative senescence in HGPS.-Hilton, B. A., Liu, J., Cartwright, B. M., Liu, Y., Breitman, M., Wang, Y., Jones, R., Tang, H., Rusinol, A., Musich, P. R., Zou, Y. Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes. © FASEB.

The effectiveness of front fork systems at damping accelerations during isolated aspects specific to cross-country mountain biking.

PubMed

Macdermid, Paul W; Miller, Matthew C; Fink, Philip W; Stannard, Stephen R

2017-11-01

Cross-country mountain bike suspension reportedly enhances comfort and performance through reduced vibration and impact exposure. This study analysed the effectiveness of three different front fork systems at damping accelerations during the crossing of three isolated obstacles (stairs, drop, and root). One participant completed three trials on six separate occasions in a randomised order using rigid, air-sprung, and carbon leaf-sprung forks. Performance was determined by time to cross obstacles, while triaxial accelerometers quantified impact exposure and damping response. Results identified significant main effect of fork type for performance time (p < 0.05). The air-sprung and leaf-sprung forks were significantly slower than the rigid forks for the stairs (p < 0.05), while air-sprung suspension was slower than the rigid for the root protocol (p < 0.05). There were no differences for the drop protocol (p < 0.05). Rigid forks reduced overall exposure (p < 0.05), specifically at the handlebars for the stairs and drop trials. More detailed analysis presented smaller vertical accelerations at the handlebar for air-sprung and leaf-sprung forks on the stairs (p < 0.05), and drop (p < 0.05) but not the root. As such, it appears that the suspension systems tested were ineffective at reducing overall impact exposure at the handlebar during isolated aspects of cross-country terrain features which may be influenced to a larger extent by rider technique.

An annotated bibliography of the hydrology and fishery studies of the South Fork Salmon River

Treesearch

Kathleen A. Seyedbagheri; Michael L. McHenry; William S. Platts

1987-01-01

A brief summary of the land management history of the South Fork Salmon River (Idaho) watershed includes citations and annotations of published and unpublished reports of fishery and hydrology studies conducted in the South Fork drainage for 1960 to 1986.

OLD SUSPENSION BRIDGE. This view appears to be looking southeast, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

OLD SUSPENSION BRIDGE. This view appears to be looking southeast, at the downstream side of the suspension bridge, which was replaced by the South Fork Trinity River Bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Impact. The southwest end is down, as the buckled middle ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Impact. The southwest end is down, as the buckled middle section splashes into the river. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Escapement and Productivity of Spring Chinook and Summer Steelhead in the John Day River Basin, Technical Report 2004-2005.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, Wayne

The objectives are: (1) Estimate number and distribution of spring Chinook salmon Oncorhynchus tshawytscha redds and spawners in the John Day River subbasin; and (2) Estimate smolt-to-adult survival rates (SAR) and out-migrant abundance for spring Chinook and summer steelhead O. mykiss and life history characteristics of summer steelhead. Spawning ground surveys for spring (stream-type) Chinook salmon were conducted in four main spawning areas (Mainstem, Middle Fork, North Fork, and Granite Creek System) and seven minor spawning areas (South Fork, Camas Creek, Desolation Creek, Trail Creek, Deardorff Creek, Clear Creek, and Big Creek) in the John Day River basin during Augustmore » and September of 2005. Census surveys included 298.2 river kilometers (88.2 rkm within index, 192.4 rkm additional within census, and 17.6 rkm within random survey areas) of spawning habitat. We observed 902 redds and 701 carcasses including 227 redds in the Mainstem, 178 redds in the Middle Fork, 420 redds in the North Fork, 62 redds in the Granite Creek System, and 15 redds in Desolation Creek. Age composition of carcasses sampled for the entire basin was 1.6% age 3, 91.2% age 4, and 7.1% age 5. The sex ratio was 57.4% female and 42.6% male. Significantly more females than males were observed in the Granite Creek System. During 2005, 82.3% of female carcasses sampled had released all of their eggs. Significantly more pre-spawn mortalities were observed in Granite Creek. Nine (1.3%) of 701 carcasses were of hatchery origin. Of 298 carcasses examined, 4.0% were positive for the presence of lesions. A significantly higher incidence of gill lesions was found in the Granite Creek System when compared to the rest of the basin. Of 114 kidney samples tested, two (1.8%) had clinical BKD levels. Both infected fish were age-4 females in the Middle Fork. All samples tested for IHNV were negative. To estimate spring Chinook and summer steelhead smolt-to-adult survival (SAR) we PIT tagged 5,138 juvenile Chinook and 4,913 steelhead during the spring of 2005. We estimated that 130,144 (95% CL's 97,133-168,409) Chinook emigrated from the upper John Day subbasin past our seining area in the Mainstem John Day River (river kilometers 274-296) between February 4 and June 16, 2005. We also estimated that 32,601 (95% CL's 29,651 and 36,264) Chinook and 47,921 (95% CL's 35,025 and 67,366) steelhead migrated past our Mainstem rotary screw trap at river kilometer (rkm) 326 between October 4, 2004 and July 6, 2005. We estimated that 20,193 (95% CL's 17,699 and 22,983) Chinook and 28,980 (95% CL's 19,914 and 43,705) steelhead migrated past our Middle Fork trap (rkm 24) between October 6, 2004 and June 17, 2005. Seventy three percent of PIT tagged steelhead migrants were age-2 fish, 13.8% were age-3, 12.7% were age-2, and 0.3% were age 4. Spring Chinook SAR for the 2002 brood year was estimated at 2.5% (100 returns of 4,000 PIT tagged smolts). Preliminary steelhead SAR (excluding 2-ocean fish) for the 2004 tagging year was estimated at 1.61% (60 returns of 3,732 PIT-tagged migrants).« less

Societal Unity of Effort, a Fork in the Road to Development or Disaster -- Considerations for State Building

DTIC Science & Technology

2006-05-25

This places it somewhere between the Structuralist and Dependency schools. International Political Economy theory balances between the Marxist and...does not portend to provide that explanation, but rather seeks to provide considerations for study in developmental theory and for United States...of globalization. Historic and contemporary developmental theories are insufficient in that they fail to account for societal characteristics in

Aftermath. The remains of the southwest end of the bridge ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Aftermath. The remains of the southwest end of the bridge lie next to the southwest pier. View is south-southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Dust trailing from the top chord, the bridge falls toward ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Dust trailing from the top chord, the bridge falls toward the river, as the southwest end (right) falls first. View southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

With water still in midflight, the northeast end of the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

With water still in mid-flight, the northeast end of the bridge (left) nears total collapse. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

76 FR 13572 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-14

.... Specifically, it addresses the following flooding sources: Left Bank Overflow Main Stem Skagit River, Left Bank Overflow Main Stem Skagit River/South Fork Skagit River, Left Bank Overflow North Fork Skagit River, Main Stem Skagit River, North Fork Skagit River, Overflow from the Main Stem Skagit River between the North...

16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070 ...

16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070 ...

Claspin Promotes Normal Replication Fork Rates in Human Cells

PubMed Central

Helleday, Thomas; Caldecott, Keith W.

2008-01-01

The S phase-specific adaptor protein Claspin mediates the checkpoint response to replication stress by facilitating phosphorylation of Chk1 by ataxia-telangiectasia and Rad3-related (ATR). Evidence suggests that these components of the ATR pathway also play a critical role during physiological S phase. Chk1 is required for high rates of global replication fork progression, and Claspin interacts with the replication machinery and might therefore monitor normal DNA replication. Here, we have used DNA fiber labeling to investigate, for the first time, whether human Claspin is required for high rates of replication fork progression during normal S phase. We report that Claspin-depleted HeLa and HCT116 cells display levels of replication fork slowing similar to those observed in Chk1-depleted cells. This was also true in primary human 1BR3 fibroblasts, albeit to a lesser extent, suggesting that Claspin is a universal requirement for high replication fork rates in human cells. Interestingly, Claspin-depleted cells retained significant levels of Chk1 phosphorylation at both Ser317 and Ser345, raising the possibility that Claspin function during normal fork progression may extend beyond facilitating phosphorylation of either individual residue. Consistent with this possibility, depletion of Chk1 and Claspin together doubled the percentage of very slow forks, compared with depletion of either protein alone. PMID:18353973

Force regulated dynamics of RPA on a DNA fork.

PubMed

Kemmerich, Felix E; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

2016-07-08

Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg(2+) concentrations, such that human RPA can melt DNA in absence of force. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Flow field and oscillatory shear stress in a tuning-fork-shaped model of the average human carotid bifurcation.

PubMed

Ding, Z; Wang, K; Li, J; Cong, X

2001-12-01

The oscillatory shear index (OSI) was developed based on the hypothesis that intimal hyperplasia was correlated with oscillatory shear stresses. However, the validity of the OSI was in question since the correlation between intimal thickness and the OSI at the side walls of the sinus in the Y-shaped model of the average human carotid bifurcation (Y-AHCB) was weak. The objectives of this paper are to examine whether the reason for the weak correlation lies in the deviation in geometry of Y-AHCB from real human carotid bifurcation, and whether this correlation is clearly improved in the tuning-fork-shaped model of the average human carotid bifurcation (TF-AHCB). The geometry of the TF-AHCB model was based on observation and statistical analysis of specimens from 74 cadavers. The flow fields in both models were studied and compared by using flow visualization methods under steady flow conditions and by using laser Doppler anemometer (LDA) under pulsatile flow conditions. The TF-shaped geometry leads to a more complex flow field than the Y-shaped geometry. This added complexity includes strengthened helical movements in the sinus, new flow separation zone, and directional changes in the secondary flow patterns. The results show that the OSI-values at the side walls of the sinus in the TF-shaped model were more than two times as large as those in the Y-shaped model. This study confirmed the stronger correlation between the OSI and intimal thickness in the tuning-fork geometry of human carotid bifurcation, and the TF-AHCB model is a significant improvement over the traditional Y-shaped model.

The Late Cretaceous Middle Fork caldera, its resurgent intrusion, and enduring landscape stability in east-central Alaska

USGS Publications Warehouse

Bacon, Charles R.; Dusel-Bacon, Cynthia; Aleinikoff, John N.; Slack, John F.

2014-01-01

The Middle Fork is a relatively well preserved caldera within a broad region of Paleozoic metamorphic rocks and Mesozoic plutons bounded by northeast-trending faults. In the relatively downdropped and less deeply exhumed crustal blocks, Cretaceous–Early Tertiary silicic volcanic rocks attest to long-term stability of the landscape. Within the Middle Fork caldera, the granite porphyry is interpreted to have been exposed by erosion of thick intracaldera tuff from an asymmetric resurgent dome. The Middle Fork of the North Fork of the Fortymile River incised an arcuate valley into and around the caldera fill on the west and north and may have cut down from within an original caldera moat. The 70 Ma land surface is preserved beneath proximal outflow tuff at the west margin of the caldera structure and beneath welded outflow tuff 16–23 km east-southeast of the caldera in a paleovalley. Within ∼50 km of the Middle Fork caldera are 14 examples of Late Cretaceous (?)–Tertiary felsic volcanic and hypabyssal intrusive rocks that range in area from <1 km2 to ∼100 km2. Rhyolite dome clusters north and northwest of the caldera occupy tectonic basins associated with northeast-trending faults and are relatively little eroded. Lava of a latite complex, 12–19 km northeast of the caldera, apparently flowed into the paleovalley of the Middle Fork of the North Fork of the Fortymile River. To the northwest of the Middle Fork caldera, in the Mount Harper crustal block, mid-Cretaceous plutonic rocks are widely exposed, indicating greater total exhumation. To the southeast of the Middle Fork block, the Mount Veta block has been uplifted sufficiently to expose a ca. 68–66 Ma equigranular granitic pluton. Farther to the southeast, in the Kechumstuk block, the flat-lying outflow tuff remnant in Gold Creek and a regionally extensive high terrace indicate that the landscape there has been little modified since 70 Ma other than entrenchment of tributaries in response to post–2.7 Ma lowering of base level of the Yukon River associated with advance of the Cordilleran ice sheet.

Effects of rainbow trout fry of a metals-contaminated diet of benthic invertebrates from the Clark Fork River, Montana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Woodward, D.F.; Brumbaugh, W.G.; DeLonay, A.J.

1994-01-01

The upper Clark Fork River in northwestern Montana has received mining wastes from the Butte and Anaconda areas since 1880. These wastes have contaminated areas of the river bed and floodplain with tailings and heavy metal sludge, resulting in elevated concentration of metals in surface water, sediments, and biota. Rainbow trout Oncorhynchus mykiss were exposed immediately after hatching for 91 d to cadmium, copper, lead, and zinc in water at concentrations simulating those in Clark Fork River. From exogenous feeding (21 d posthatch) through 91 d, fry were also fed benthic invertebrates from the Clark Fork River that contained elevatedmore » concentrations of arsenic, cadmium, copper, and lead. Evaluations of different combinations of diet and water exposure indicated diet-borne metals were more important than water-borne metals - at the concentrations we tested - in reducing survival and growth of rainbow trout. Whole-body metal concentrations ([mu]g/g, wet weight) at 91 d in fish fed Clark Fork invertebrates without exposure to Clark Fork water were arsenic, 1.4; cadmium, 0.16; and copper, 6.7. These were similar to concentrations found in Clark Fork River fishes. Livers from fish on the high-metals diets exhibited degenerative changes and generally lacked glycogen vacuolation. Indigenous Clark Fork River invertebrates provide a concentrated source of metals for accumulation into young fishes, and probably were the cause of decreased survival and growth of age-0 rainbow trout in our laboratory exposures. 30 refs., 8 figs., 4 tabs.« less

76 FR 53082 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-25

..., 201 Saint Andrews Street, Tarboro, NC 27886. Smith County, Texas, and Incorporated Areas Black Fork.... Tributary BF-1 At the Black Fork Creek +434 +436 City of Tyler, confluence. Unincorporated Areas of Smith County. Approximately 1.2 miles None +476 upstream of Loop 323. Tributary BF-M-1 At the Black Fork Creek...

With deck removed and critical members severed, the bridge begins ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

With deck removed and critical members severed, the bridge begins to succumb to tractors, pulling cables attached to its downstream side. View is to the southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Spray and dust hang in the air, as the demolition ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Spray and dust hang in the air, as the demolition is completed. Only the southeast pier remains standing at right. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

76 FR 70397 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-14

... the Ohio River +549 +550 Town of Fort Gay, Unincorporated Areas of Wayne County. confluence. At the... of Fort Gay. Fork). confluence to approximately 1.1 miles upstream of the Tug Fork confluence. Tug Fork At the Big Sandy River +576 +575 Town of Fort Gay. confluence. Approximately 0.5 mile +577 +575...

Integrating Salmon Recovery, Clean Water Act Compliance, Restoration, and Climate Change Impacts in the South Fork Nooksack River

EPA Science Inventory

"The South Fork Nooksack River (SFNR) is an important tributary to the Nooksack River, Bellingham Bay, and the Salish Sea. The South Fork Nooksack River comprises one of the 22 independent populations of spring Chinook in the Puget Sound Chinook Evolutionarily Significant Un...

76 FR 46721 - Salmon-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-03

...-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project Environmental Impact... improve the health of the ecosystem and reach the desired future condition. DATES: Comments concerning the... Ecosystem Restoration Project EIS, P.O. Box 180, 11 Casey Rd., North Fork, ID 83466. Comments may also be...

75 FR 57493 - Notice of Inventory Completion: Department of Anthropology & Ethnic Studies, University of Nevada...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-21

..., Nevada; Shoshone-Paiute Tribes of the Duck Valley Reservation, Nevada; South Fork Band (Constituent Band... of Idaho; Shoshone-Paiute Tribes of the Duck Valley Reservation, Nevada; South Fork Band (Constituent... Idaho; Shoshone-Paiute Tribes of the Duck Valley Reservation, Nevada; South Fork Band; Summit Lake...

76 FR 35009 - Draft Oil and Gas Management Plan/Environmental Impact Statement for Big South Fork National...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-15

... DEPARTMENT OF THE INTERIOR National Park Service [5130-0400-NZM] Draft Oil and Gas Management Plan... Management Plan/ Environmental Impact Statement for Big South Fork National River and Recreation Area and... gas management plan/environmental impact statement (OGMP/DEIS) for the proposed Big South Fork...

Training Guidelines: Fork Lift Truck Driving.

ERIC Educational Resources Information Center

Ceramics, Glass, and Mineral Products Industry Training Board, Harrow (England).

This manual of operative training guidelines for fork lift truck driving has been developed by the Ceramics, Glass and Mineral Products Industry Training Board (Great Britain) in consultation with a number of firms which manufacture fork lift trucks or which already have training--programs for their use. The purpose of the guidelines is to assist…

HOWARD FORK ACID ROCK DRAINAGE SOURCE INTERCEPTION STUDY; HOWARD FORK OF THE SAN MIGUEL RIVER NEAR OPHIR, COLORADO

EPA Science Inventory

This project proposes to analyze regional hydrogeology as it relates to mine workings which discharge significant heavy metals into the Howard Fork of the San Miguel River and recommend strategies to intercept and divert water away from mineralized zones. The study also includes...

Chk1 promotes replication fork progression by controlling replication initiation

PubMed Central

Petermann, Eva; Woodcock, Mick; Helleday, Thomas

2010-01-01

DNA replication starts at initiation sites termed replication origins. Metazoan cells contain many more potential origins than are activated (fired) during each S phase. Origin activation is controlled by the ATR checkpoint kinase and its downstream effector kinase Chk1, which suppresses origin firing in response to replication blocks and during normal S phase by inhibiting the cyclin-dependent kinase Cdk2. In addition to increased origin activation, cells deficient in Chk1 activity display reduced rates of replication fork progression. Here we investigate the causal relationship between increased origin firing and reduced replication fork progression. We use the Cdk inhibitor roscovitine or RNAi depletion of Cdc7 to inhibit origin firing in Chk1-inhibited or RNAi-depleted cells. We report that Cdk inhibition and depletion of Cdc7 can alleviate the slow replication fork speeds in Chk1-deficient cells. Our data suggest that increased replication initiation leads to slow replication fork progression and that Chk1 promotes replication fork progression during normal S phase by controlling replication origin activity. PMID:20805465

Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation

NASA Astrophysics Data System (ADS)

Min, Wookee; Bruhn, Christopher; Grigaravicius, Paulius; Zhou, Zhong-Wei; Li, Fu; Krüger, Anja; Siddeek, Bénazir; Greulich, Karl-Otto; Popp, Oliver; Meisezahl, Chris; Calkhoven, Cornelis F.; Bürkle, Alexander; Xu, Xingzhi; Wang, Zhao-Qi

2013-12-01

Damaged replication forks activate poly(ADP-ribose) polymerase 1 (PARP1), which catalyses poly(ADP-ribose) (PAR) formation; however, how PARP1 or poly(ADP-ribosyl)ation is involved in the S-phase checkpoint is unknown. Here we show that PAR, supplied by PARP1, interacts with Chk1 via a novel PAR-binding regulatory (PbR) motif in Chk1, independent of ATR and its activity. iPOND studies reveal that Chk1 associates readily with the unperturbed replication fork and that PAR is required for efficient retention of Chk1 and phosphorylated Chk1 at the fork. A PbR mutation, which disrupts PAR binding, but not the interaction with its partners Claspin or BRCA1, impairs Chk1 and the S-phase checkpoint activation, and mirrors Chk1 knockdown-induced hypersensitivity to fork poisoning. We find that long chains, but not short chains, of PAR stimulate Chk1 kinase activity. Collectively, we disclose a previously unrecognized mechanism of the S-phase checkpoint by PAR metabolism that modulates Chk1 activity at the replication fork.

Role of replication protein A as sensor in activation of the S-phase checkpoint in Xenopus egg extracts

PubMed Central

Recolin, Bénédicte; Van Der Laan, Siem; Maiorano, Domenico

2012-01-01

Uncoupling between DNA polymerases and helicase activities at replication forks, induced by diverse DNA lesions or replication inhibitors, generate long stretches of primed single-stranded DNA that is implicated in activation of the S-phase checkpoint. It is currently unclear whether nucleation of the essential replication factor RPA onto this substrate stimulates the ATR-dependent checkpoint response independently of its role in DNA synthesis. Using Xenopus egg extracts to investigate the role of RPA recruitment at uncoupled forks in checkpoint activation we have surprisingly found that in conditions in which DNA synthesis occurs, RPA accumulation at forks stalled by either replication stress or UV irradiation is dispensable for Chk1 phosphorylation. In contrast, when both replication fork uncoupling and RPA hyperloading are suppressed, Chk1 phosphorylation is inhibited. Moreover, we show that extracts containing reduced levels of RPA accumulate ssDNA and induce spontaneous, caffeine-sensitive, Chk1 phosphorylation in S-phase. These results strongly suggest that disturbance of enzymatic activities of replication forks, rather than RPA hyperloading at stalled forks, is a critical determinant of ATR activation. PMID:22187152

A single active nanoelectromechanical tuning fork front-end radio-frequency receiver

NASA Astrophysics Data System (ADS)

Bartsch, Sebastian T.; Rusu, A.; Ionescu, Adrian M.

2012-06-01

Nanoelectromechanical systems (NEMS) offer the potential to revolutionize fundamental methods employed for signal processing in today’s telecommunication systems, owing to their spectral purity and the prospect of integration with existing technology. In this work we present a novel, front-end receiver topology based on a single device silicon nanoelectromechanical mixer-filter. The operation is demonstrated by using the signal amplification in a field effect transistor (FET) merged into a tuning fork resonator. The combination of both a transistor and a mechanical element into a hybrid unit enables on-chip functionality and performance previously unachievable in silicon. Signal mixing, filtering and demodulation are experimentally demonstrated at very high frequencies ( > 100 MHz), maintaining a high quality factor of Q = 800 and stable operation at near ambient pressure (0.1 atm) and room temperature (T = 300 K). The results show that, ultimately miniaturized, silicon NEMS can be utilized to realize multi-band, single-chip receiver systems based on NEMS mixer-filter arrays with reduced system complexity and power consumption.

Water-Quality, Bed-Sediment, and Biological Data (October 2007 through September 2008) and Statistical Summaries of Long-Term Data for Streams in the Clark Fork Basin, Montana

USGS Publications Warehouse

Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

2009-01-01

Water, bed sediment, and biota were sampled in streams from Butte to near Missoula as part of a long-term monitoring program in the upper Clark Fork basin; additional water samples were collected in the Clark Fork basin from sites near Missoula downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water samples were collected periodically at 23 sites from October 2007 through September 2008. Bed-sediment and biota samples were collected once at 13 sites during August 2008. This report presents the analytical results and quality assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2007 through September 2008. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Turbidity was analyzed for water samples collected at sites where seasonal daily values of turbidity were being determined and at Clark Fork above Missoula. Nutrients also were analyzed at all the supplemental water-quality sites, except for Clark Fork Bypass, near Bonner. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for four sites, and seasonal daily values of turbidity were determined for four sites. Bed-sediment data include trace-element concentrations in the fine-grained fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

Water-quality, bed-sediment, and biological data (October 2008 through September 2009) and statistical summaries of long-term data for streams in the Clark Fork basin, Montana

USGS Publications Warehouse

Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

2010-01-01

Water, bed sediment, and biota were sampled in streams from Butte to near Missoula, Montana, as part of a long-term monitoring program in the upper Clark Fork basin; additional water samples were collected in the Clark Fork basin from sites near Missoula downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted by the U.S. Geological Survey in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water samples were collected periodically at 24 sites from October 2008 through September 2009. Bed-sediment and biota samples were collected once at 13 sites during August 2009. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2008 through September 2009. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Turbidity was analyzed for water samples collected at the four sites where seasonal daily values of turbidity were being determined as well as at Clark Fork above Missoula. Nutrients also were analyzed at all the supplemental water-quality sites, except for Clark Fork Bypass, near Bonner. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for four sites. Bed-sediment data include trace-element concentrations in the fine-grained fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

The Human RNA Polymerase I Transcription Terminator Complex Acts as a Replication Fork Barrier That Coordinates the Progress of Replication with rRNA Transcription Activity.

PubMed

Akamatsu, Yufuko; Kobayashi, Takehiko

2015-05-01

In S phase, the replication and transcription of genomic DNA need to accommodate each other, otherwise their machineries collide, with chromosomal instability as a possible consequence. Here, we characterized the human replication fork barrier (RFB) that is present downstream from the 47S pre-rRNA gene (ribosomal DNA [rDNA]). We found that the most proximal transcription terminator, Sal box T1, acts as a polar RFB, while the other, Sal box T4/T5, arrests replication forks bidirectionally. The fork-arresting activity at these sites depends on polymerase I (Pol I) transcription termination factor 1 (TTF-1) and a replisome component, TIMELESS (TIM). We also found that the RFB activity was linked to rDNA copies with hypomethylated CpG and coincided with the time that actively transcribed rRNA genes are replicated. Failed fork arrest at RFB sites led to a slowdown of fork progression moving in the opposite direction to rRNA transcription. Chemical inhibition of transcription counteracted this deceleration of forks, indicating that rRNA transcription impedes replication in the absence of RFB activity. Thus, our results reveal a role of RFB for coordinating the progression of replication and transcription activity in highly transcribed rRNA genes. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

76 FR 34690 - Placer County Water Agency; Notice of Application Accepted for Filing, Soliciting Motions To...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-14

... Water Agency; Notice of Application Accepted for Filing, Soliciting Motions To Intervene and Protests... Water Agency. e. Name of Project: Middle Fork American River Project. f. Location: The Middle Fork....S. Department of Agriculture-- Forest Service. g. Filed Pursuant to: Federal Power Act 16 U.S.C. 791...

Qualitative Assessment: Evaluating the Impacts of Climate Change on Endangered Species Act Recovery Actions for the South Fork Nooksack River, WA

EPA Science Inventory

The South Fork Nooksack River (South Fork) is located in northwest Washington State and is home to nine species of Pacific salmon, including Nooksack early Chinook (aka, spring Chinook salmon), an iconic species for the Nooksack Indian Tribe. The quantity of salmon in the South F...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean...

76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-20

... Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the National... Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park Service... services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not to exceed 3...

9. 'CRIB DAM IN LAKE FORK RIVER AT HEADING OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. 'CRIB DAM IN LAKE FORK RIVER AT HEADING OF LAKE FORK CANAL, UINTAH PROJECT. TWO SLUICEWAYS TWENTY FEET WIDE HAVE BEEN LEFT IN THE DAM TO PASS BOULDERS DURING HIGH WATER. THESE SLUICEWAYS ARE CLOSED BY LOGS AND HAY DURING LOW WATER.' Date unknown - Irrigation Canals in the Uinta Basin, Duchesne, Duchesne County, UT

16 CFR 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Requirements for fork and frame assembly... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

16 CFR 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Requirements for fork and frame assembly... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

16 CFR 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for fork and frame assembly... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

16 CFR 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Requirements for fork and frame assembly... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

Calibration of Speed Enforcement Down-The-Road Radars

PubMed Central

Jendzurski, John; Paulter, Nicholas G.

2009-01-01

We examine the measurement uncertainty associated with different methods of calibrating the ubiquitous down-the-road (DTR) radar used in speed enforcement. These calibration methods include the use of audio frequency sources, tuning forks, a fifth wheel attached to the rear of the vehicle with the radar unit, and the speedometer of the vehicle. We also provide an analysis showing the effect of calibration uncertainty on DTR-radar speed measurement uncertainty. PMID:27504217

Phytotoxicity of floodplain soils contaminated with trace metals along the Clark Fork River, Grant-Kohrs Ranch National Historic Site, Deer Lodge, Montana, USA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rader, B.R.; Nimmo, D.W.R.; Chapman, P.L.

1997-07-01

Concentrations of metals in sediments and soils deposited along the floodplain of the Clark Fork River, within the Grant-Kohrs Ranch National Historic Site, Deer Lodge, Montana, USA, have exceeded maximum background concentrations in the United States for most metals tested. As a result of mining and smelting activities, portions of the Deer Lodge Valley, including the Grant-Kohrs Ranch, have received National Priority List Designation under the Comprehensive Environmental Response, Compensation and Liability Act. Using a series of plant germination tests, pH measurements, and metal analyses, this study investigated the toxicity of soils from floodplain slicken areas, bare spots devoid ofmore » vegetation, along the Clark Fork River. The slicken soils collected from the Grant-Kohrs Ranch were toxic to all four plant species tested. The most sensitive endpoint in the germination tests was root length and the least sensitive was emergence. Considering emergence, the most sensitive species was the resident grass species Agrostis gigantea. The sensitivities were reversed when root lengths were examined, with Echinochloa crusgalli showing the greatest sensitivity. Both elevated concentrations of metals and low pH were necessary to produce an acutely phytotoxic response in laboratory seed germination tests using slicken soils. Moreover, pH values on the Grant-Kohrs Ranch appear to be a better predictor of acutely phytotoxic conditions than total metal levels.« less

Utilization of protein expression profiles as indicators of environmental impairment of smallmouth bass (Micropterus dolomieu) from the Shenandoah River, Virginia, USA.

PubMed

Ripley, Jennifer; Iwanowicz, Luke; Blazer, Vicki; Foran, Christy

2008-08-01

The Shenandoah River (VA, USA), the largest tributary of the Potomac River (MD, USA) and an important source of drinking water, has been the site of extensive fish kills since 2004. Previous investigations indicate environmental stressors may be adversely modulating the immune system of smallmouth bass (Micropterus dolomieu) and other species. Anterior kidney (AK) tissue, the major site of blood cell production in fish, was collected from smallmouth bass at three sites along the Shenandoah River. The tissue was divided for immune function and proteomics analyses. Bactericidal activity and respiratory burst were significantly different between North Fork and mainstem Shenandoah River smallmouth bass, whereas South Fork AK tissue did not significantly differ in either of these measures compared with the other sites. Cytotoxic cell activity was highest among South Fork and lowest among North Fork AK leukocytes. The composite two-dimension gels of the North Fork and mainstem smallmouth bass AK tissues contained 584 and 591 spots, respectively. South Fork smallmouth bass AK expressed only 335 proteins. Nineteen of 50 proteins analyzed by matrix-assisted laser desorption ionization-time of flight were successfully identified. Three of the four identified proteins with increased expression in South Fork AK tissue were involved in metabolism. Seven proteins exclusive to mainstem and North Fork smallmouth bass AK and expressed at comparable abundances serve immune and stress response functions. The proteomics data indicate these fish differ in metabolic capacity of AK tissue and in the ability to produce functional leukocytes. The variable responses of the immune function assays further indicate disruption to the immune system. Our results allow us to hypothesize underlying physiological changes that may relate to fish kills and suggest relevant contaminants known to produce similar physiological disruption.

Utilization of protein expression profiles as indicators of environmental impairment of smallmouth bass (Micropterus dolomieu) from the Shenandoah River, Virginia, USA

USGS Publications Warehouse

Ripley, J.; Iwanowicz, L.; Blazer, V.; Foran, C.

2008-01-01

The Shenandoah River (VA, USA), the largest tributary of the Potomac River (MD, USA) and an important source of drinking water, has been the site of extensive fish kills since 2004. Previous investigations indicate environmental stressors may be adversely modulating the immune system of smallmouth bass (Micropterus dolomieu) and other species. Anterior kidney (AK) tissue, the major site of blood cell production in fish, was collected from smallmouth bass at three sites along the Shenandoah River. The tissue was divided for immune function and proteomics analyses. Bactericidal activity and respiratory burst were significantly different between North Fork and mainstem Shenandoah River smallmouth bass, whereas South Fork AK tissue did not significantly differ in either of these measures compared with the other sites. Cytotoxic cell activity was highest among South Fork and lowest among North Fork AK leukocytes. The composite two-dimension gels of the North Fork and mainstem smallmouth bass AK tissues contained 584 and 591 spots, respectively. South Fork smallmouth bass AK expressed only 335 proteins. Nineteen of 50 proteins analyzed by matrix-assisted laser desorption ionization-time of flight were successfully identified. Three of the four identified proteins with increased expression in South Fork AK tissue were involved in metabolism. Seven proteins exclusive to mainstem and North Fork smallmouth bass AK and expressed at comparable abundances serve immune and stress response functions. The proteomics data indicate these fish differ in metabolic capacity of AK tissue and in the ability to produce functional leukocytes. The variable responses of the immune function assays further indicate disruption to the immune system. Our results allow us to hypothesize underlying physiological changes that may relate to fish kills and suggest relevant contaminants known to produce similar physiological disruption. ?? 2008 SETAC.

A Galaxy at the Center of the Hubble Tuning Fork

NASA Image and Video Library

2017-12-08

This galaxy is known as Mrk 820 and is classified as a lenticular galaxy — type S0 on the Hubble Tuning Fork. The Hubble Tuning Fork is used to classify galaxies according to their morphology. Elliptical galaxies look like smooth blobs in the sky and lie on the handle of the fork. They are arranged along the handle based on how elliptical they are, with the more spherical galaxies furthest from the tines of the fork, and the more egg-shaped ones closest to the end of the handle where it divides. The two prongs of the tuning fork represent types of unbarred and barred spiral galaxies. Lenticular galaxies like Mrk 820 are in the transition zone between ellipticals and spirals and lie right where the fork divides. A closer look at the appearance of Mrk 820 reveals hints of a spiral structure embedded in a circular halo of stars. Surrounding Mrk 820 in this image is a good sampling of other galaxy types, covering almost every type found on the Hubble Tuning Fork, both elliptical and spiral. Most of the smears and specks are distant galaxies, but the prominent bright object at the bottom is a foreground star called TYC 4386-787-1. Credit: ESA/Hubble & NASA and N. Gorin (STScI), Acknowledgement: Judy Schmidt NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Tuning fork enhanced interferometric photoacoustic spectroscopy: a new method for trace gas analysis

NASA Astrophysics Data System (ADS)

Köhring, M.; Pohlkötter, A.; Willer, U.; Angelmahr, M.; Schade, W.

2011-01-01

A photoacoustic trace gas sensor based on an optical read-out method of a quartz tuning fork is shown. Instead of conventional piezoelectric signal read-out, as applied in well-known quartz-enhanced photoacoustic spectroscopy (QEPAS), an interferometric read-out method for measurement of the tuning fork's oscillation is presented. To demonstrate the potential of the optical read-out of tuning forks in photoacoustics, a comparison between the performances of a sensor with interferometric read-out and conventional QEPAS with piezoelectric read-out is reported. The two sensors show similar characteristics. The detection limit (L) for the optical read-out is determined to be L opt=(2598±84) ppm (1 σ) compared to L elec=(2579±78) ppm (1 σ) for piezoelectric read-out. In both cases the detection limit is defined by the thermal noise of the tuning fork.

ATR prohibits replication catastrophe by preventing global exhaustion of RPA.

PubMed

Toledo, Luis Ignacio; Altmeyer, Matthias; Rask, Maj-Britt; Lukas, Claudia; Larsen, Dorthe Helena; Povlsen, Lou Klitgaard; Bekker-Jensen, Simon; Mailand, Niels; Bartek, Jiri; Lukas, Jiri

2013-11-21

ATR, activated by replication stress, protects replication forks locally and suppresses origin firing globally. Here, we show that these functions of ATR are mechanistically coupled. Although initially stable, stalled forks in ATR-deficient cells undergo nucleus-wide breakage after unscheduled origin firing generates an excess of single-stranded DNA that exhausts the nuclear pool of RPA. Partial reduction of RPA accelerated fork breakage, and forced elevation of RPA was sufficient to delay such "replication catastrophe" even in the absence of ATR activity. Conversely, unscheduled origin firing induced breakage of stalled forks even in cells with active ATR. Thus, ATR-mediated suppression of dormant origins shields active forks against irreversible breakage via preventing exhaustion of nuclear RPA. This study elucidates how replicating genomes avoid destabilizing DNA damage. Because cancer cells commonly feature intrinsically high replication stress, this study also provides a molecular rationale for their hypersensitivity to ATR inhibitors. Copyright © 2013 Elsevier Inc. All rights reserved.

Habitat-dependent interactions between two size-classes of juvenile steelhead in a small stream

Treesearch

Bret C. Harvey; Rodney J. Nakamoto

1997-01-01

Abstract - The presence of small steelhead (Oncorhynchus mykiss; averaging 55 mm fork length) influenced the growth of larger juvenile steelhead (90 mm fork length) during a 6-week experiment conducted in North Fork Caspar Creek, California, in summer 1994. In fenced replicate deep stream sections in this small stream, growth of the larger steelhead was greater in...

Status of the Mussel Resource in Little South Fork Cumberland River

Treesearch

Melvin L. Warren; Wendell R. Haag; Brooks M. Burr

1999-01-01

As recently as the 198Os, the Little South Fork Cumberland River of southeastern Kentucky supported a diverse freshwater mussel fauna (Starnes and Bogan 1982; Appendix A). The Little South Fork represented one of the last rivers to support a high number of mussel species in the Cumberland River drainage of Kentucky and Tennessee. The river was first surveyed...

A watershed's response to logging and roads: South Fork of Caspar Creek, California, 1967-1976

Treesearch

Raymond M. Rice; Forest B. Tilley; Patricia A. Datzman

1979-01-01

The effect of logging and roadbuilding on erosion and sedimentation are analyzed by comparing the North Fork and South Fork of Caspar Creek, in northern California. Increased sediment production during the 4 years after road construction, was 326 cu yd/sq mi/yr—80 percent greater than that predicted by the predisturbance regression analysis. The average...

16 CFR § 1512.14 - Requirements for fork and frame assembly.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Requirements for fork and frame assembly. Â... assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N (200 lbf) or at least 39.5 J (350 in-lb) of energy, whichever results in the greater force, in accordance...

Gas Phase Photoacoustic Sensor at 8.41 mu m Using Quartz Tuning Forks and Amplitude Modulated Quantum Cascade Lasers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wojcik, Michael D.; Phillips, Mark C.; Cannon, Bret D.

2006-10-01

We demonstrate the performance of a novel long-wave infrared photoacoustic laser absorbance spectrometer for gas-phase species using an amplitude modulated (AM) quantum cascade (QC) laser and a quartz tuning fork microphone. Photoacoustic signal was generated by focusing the output of a Fabry-Perot QC laser operating at 8.41 ?m between the legs of a quartz tuning fork which served as a transducer for the transient acoustic pressure wave. The QC laser was modulated at the resonant frequency of the tuning fork (32.8 kHz) and delivered a modest 5.3 mW at the tuning fork. This spectrometer was calibrated using the infrared absorbermore » Freon-134a by performing a simultaneous absorption measurement using a 35 cm absorption cell. The NEAS of this instrument was determined to be 2 x 10{sup -8} W cm-1 Hz{sup -1/2}. A corresponding theoretical analysis of the instrument sensitivity is presented and is capable of quantitatively reproducing the experimental NEAS, indicating that the fundamental sensitivity of this technique is limited by the noise floor of the tuning fork itself.« less

Evaluate Status of Pacific Lamprey in the Clearwater River Drainage, Idaho, Annual Report 2002.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cochnauer, Tim; Claire, Christopher

In 2002 Idaho Department of Fish and Game continued investigation into the status of Pacific lamprey populations in Idaho's Clearwater River drainage. Trapping, electrofishing, and spawning ground redd surveys were used to determine Pacific lamprey distribution, life history strategies, and habitat requirements in the South Fork Clearwater River, Lochsa River, Selway River, and Middle Fork Clearwater River subbasins. Five-hundred forty-one ammocoetes were captured electroshocking 70 sites in the South Fork Clearwater River, Lochsa River, Selway River, Middle Fork Clearwater River, Clearwater River, and their tributaries in 2002. Habitat utilization surveys in Red River support previous work indicating Pacific lamprey ammocoetemore » densities are greater in lateral scour pool habitats compared to riffles and rapids. Presence-absence survey findings in 2002 augmented 2000 and 2001 indicating Pacific lamprey macrothalmia and ammocoetes are not numerous or widely distributed. Pacific lamprey distribution was confined to the lower reaches of Red River below rkm 8.0, the South Fork Clearwater River, Lochsa River (Ginger Creek to mouth), Selway River (Race Creek to mouth), Middle Fork Clearwater River, and the Clearwater River (downstream to Potlatch River).« less

Geothermal observation wells, Mt. Hood, Oregon. Final report, October 4, 1977-July 9, 1979

DOE Office of Scientific and Technical Information (OSTI.GOV)

Covert, W.F.; Meyer, H.J.

1979-11-01

Exploration drilling operations were conducted which included the deepening of an existing hole, designated as Old Maid Flat No. 1, from 1850 ft (564 m) to 4002 (1220 m) on the western approaches to Mt. Hood and the drilling of three new holes ranging from 940 ft (287 m) to 1340 ft (409 m). The Clear Fork hole, located in Old Maid Flat, was drilled to 1320 ft (402 m). The Zigzag hole was drilled to 940 ft (287 m) at the southwestern base of Mt. Hood in the Zigzag River valley. The remaining hole was drilled on the Timberlinemore » Lodge grounds which is on the south flank of Mt. Hood at an elevation of about 6000 ft (1829 m) above sea level. The deepening project designated as Old Maid Flat No. 1 encountered a maximum bottom hole temperature of about 180/sup 0/F (82/sup 0/C) and is to this date the deepest exploratory hole in the Mt. Hood vicinity. No significant drilling problems were encountered. The Clear Fork and Zigzag River holes were completed without significant problems. The Timberline Lodge hole encountered severe drilling conditions, including unconsolidated formations. Two strings of tools were left in the hole from structural collapse of the hole. The hole was scheduled as a 2000 ft (610 m) test. Drilling did not proceed beyond 1350 ft (412 m) and due to junk it was unobstructed to a depth of 838 ft (255 m). Observation pipe was installed to 735 ft (224 m) due to further disintegration of the hole. The work was prematurely terminated due to weather conditions.« less

Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination

PubMed Central

Fredsøe, Jacob; Nielsen, Ida; Pedersen, Jakob Madsen; Bentsen, Iben Bach; Lisby, Michael; Bjergbaek, Lotte; Andersen, Anni H

2015-01-01

Faithful DNA replication with correct termination is essential for genome stability and transmission of genetic information. Here we have investigated the potential roles of Topoisomerase II (Top2) and the RecQ helicase Sgs1 during late stages of replication. We find that cells lacking Top2 and Sgs1 (or Top3) display two different characteristics during late S/G2 phase, checkpoint activation and accumulation of asymmetric X-structures, which are both independent of homologous recombination. Our data demonstrate that checkpoint activation is caused by a DNA structure formed at the strongest rDNA replication fork barrier (RFB) during replication termination, and consistently, checkpoint activation is dependent on the RFB binding protein, Fob1. In contrast, asymmetric X-structures are formed independent of Fob1 at less strong rDNA replication fork barriers. However, both checkpoint activation and formation of asymmetric X-structures are sensitive to conditions, which facilitate fork merging and progression of replication forks through replication fork barriers. Our data are consistent with a redundant role of Top2 and Sgs1 together with Top3 (Sgs1-Top3) in replication fork merging at rDNA barriers. At RFB either Top2 or Sgs1-Top3 is essential to prevent formation of a checkpoint activating DNA structure during termination, but at less strong rDNA barriers absence of the enzymes merely delays replication fork merging, causing an accumulation of asymmetric termination structures, which are solved over time. PMID:26630413

Environmental Assessment Deicer Recovery at Grand Forks AFB, North Dakota

DTIC Science & Technology

2004-12-15

Air Force Base (AFB), North Dakota. Contacts: 319 CES/CEVA 525 Tuskegee Airmen Boulevard (Blvd) Grand Forks AFB, ND...ACRONYMS, ABBREVIATIONS, AND TERMS AAM Annual Arithmetic Mean ACM Asbestos Containing Material AFB Air Force Base AFI Air Force Instruction AICUZ...meter 10 GFAFB Grand Forks Air Force Base HAP Hazardous Air Pollutants hr Hour H2S Hydrogen Sulfide IRP Installation Restoration

Continuous water-quality monitoring and regression analysis to estimate constituent concentrations and loads in the Red River of the North at Fargo and Grand Forks, North Dakota, 2003-12

USGS Publications Warehouse

Galloway, Joel M.

2014-01-01

The Red River of the North (hereafter referred to as “Red River”) Basin is an important hydrologic region where water is a valuable resource for the region’s economy. Continuous water-quality monitors have been operated by the U.S. Geological Survey, in cooperation with the North Dakota Department of Health, Minnesota Pollution Control Agency, City of Fargo, City of Moorhead, City of Grand Forks, and City of East Grand Forks at the Red River at Fargo, North Dakota, from 2003 through 2012 and at Grand Forks, N.Dak., from 2007 through 2012. The purpose of the monitoring was to provide a better understanding of the water-quality dynamics of the Red River and provide a way to track changes in water quality. Regression equations were developed that can be used to estimate concentrations and loads for dissolved solids, sulfate, chloride, nitrate plus nitrite, total phosphorus, and suspended sediment using explanatory variables such as streamflow, specific conductance, and turbidity. Specific conductance was determined to be a significant explanatory variable for estimating dissolved solids concentrations at the Red River at Fargo and Grand Forks. The regression equations provided good relations between dissolved solid concentrations and specific conductance for the Red River at Fargo and at Grand Forks, with adjusted coefficients of determination of 0.99 and 0.98, respectively. Specific conductance, log-transformed streamflow, and a seasonal component were statistically significant explanatory variables for estimating sulfate in the Red River at Fargo and Grand Forks. Regression equations provided good relations between sulfate concentrations and the explanatory variables, with adjusted coefficients of determination of 0.94 and 0.89, respectively. For the Red River at Fargo and Grand Forks, specific conductance, streamflow, and a seasonal component were statistically significant explanatory variables for estimating chloride. For the Red River at Grand Forks, a time component also was a statistically significant explanatory variable for estimating chloride. The regression equations for chloride at the Red River at Fargo provided a fair relation between chloride concentrations and the explanatory variables, with an adjusted coefficient of determination of 0.66 and the equation for the Red River at Grand Forks provided a relatively good relation between chloride concentrations and the explanatory variables, with an adjusted coefficient of determination of 0.77. Turbidity and streamflow were statistically significant explanatory variables for estimating nitrate plus nitrite concentrations at the Red River at Fargo and turbidity was the only statistically significant explanatory variable for estimating nitrate plus nitrite concentrations at Grand Forks. The regression equation for the Red River at Fargo provided a relatively poor relation between nitrate plus nitrite concentrations, turbidity, and streamflow, with an adjusted coefficient of determination of 0.46. The regression equation for the Red River at Grand Forks provided a fair relation between nitrate plus nitrite concentrations and turbidity, with an adjusted coefficient of determination of 0.73. Some of the variability that was not explained by the equations might be attributed to different sources contributing nitrates to the stream at different times. Turbidity, streamflow, and a seasonal component were statistically significant explanatory variables for estimating total phosphorus at the Red River at Fargo and Grand Forks. The regression equation for the Red River at Fargo provided a relatively fair relation between total phosphorus concentrations, turbidity, streamflow, and season, with an adjusted coefficient of determination of 0.74. The regression equation for the Red River at Grand Forks provided a good relation between total phosphorus concentrations, turbidity, streamflow, and season, with an adjusted coefficient of determination of 0.87. For the Red River at Fargo, turbidity and streamflow were statistically significant explanatory variables for estimating suspended-sediment concentrations. For the Red River at Grand Forks, turbidity was the only statistically significant explanatory variable for estimating suspended-sediment concentration. The regression equation at the Red River at Fargo provided a good relation between suspended-sediment concentration, turbidity, and streamflow, with an adjusted coefficient of determination of 0.95. The regression equation for the Red River at Grand Forks provided a good relation between suspended-sediment concentration and turbidity, with an adjusted coefficient of determination of 0.96.

Gold placers of the historical Fortymile River region, Alaska

USGS Publications Warehouse

Yeend, Warren E.

1996-01-01

The Fortymile River region in east-central Alaska has a long and colorful history as the site of the first major gold discovery in interior Alaska. Placer gold has been mined in the region nearly every year since its original discovery in 1886. Total gold production is approximately 500,000 troy ounces. Although many of the rich deposits have been mined, there still exist areas that contain gold. Areas of mined and unmined gold-bearing creek and terrace gravels are outlined on the accompanying geologic map. The early history of the Fortymile area centered on the small frontier settlement of Fortymile City located at the junction of the Fortymile and Yukon Rivers in Canadian territory. This was the supply and jumping-off point for prospectors who worked their way into Alaska up the Fortymile River and found gold on many of its tributaries. Hand mining, both underground and surface, using sluice boxes and (or) rockers were the earliest methods; later, hydraulicking, dredging, and draglining methods were used. More recently, bulldozers and elevated trammels have been used, as well as very portable floating suction dredges. The rich mining lore of the area is closely associated with events of the nearby world-famous Klondike District. Bedrock and placer geology and mining history of individual gold-rich creeks are herein updated. The Fortymile area, which is part of the Yukon-Tanana Upland, contains quartzite, schist, gneiss, amphibolite, marble, serpentinite, and granite overlain by basalt, sandstone, conglomerate, shale, tuff, and coal; overlying these rocks are several deposits of varying ages consisting of gold-bearing gravel and colluvium. The close spatial association of creeks containing placer gold and the gneiss, schist, amphibolite, and marble unit strongly suggests this metamorphic unit is the gold source. High terrace gravels record a time from the late Tertiary to early Pleistocene when the ancestral Fortymile River and its major tributaries, the North and South Forks, had floodplains roughly 1 to 2 miles (2-3 kilometers) wide and gradients of about 4 feet per mile (0.75 meters per kilometer). Base-level lowering during the post-early Pleistocene caused the rivers to cut into their floodplains and to develop the youthful characteristics they have today such as V-shaped canyons, narrow floodplains, and gradients of at least twice those of the old river. Colluvium marginal to creek deposits in steep-sided valleys is often gold bearing. Much of the unconsolidated gravel within the major drainages of the Fortymile River, South Fork, North Fork, and Mosquito Fork is colluvium.Heavy-mineral-concentrate samples from the gold-producing creeks and high terrace gravels contain varying amounts of magnetite (20 to 80 percent) and ilmenite (10 to 30 percent), and samples from creeks draining areas principally composed of metamorphic rocks contain abundant garnet (10 to 30 percent). Gold fineness ranges from 620 to 927, but it is difficult to attach any geologic significance to the fineness data.Most placer gold in the Fortymile River area has been recovered at, or near, the gravelbedrock contact. The lowermost 3.3 feet (1 meter) of gravel and the uppermost 1.6 feet (0.5 meter) of bedrock may contain as much as 80 to 90 percent of the gold that is ultimately recovered. Gold nuggets are rare and most of the gold recovered is in the form of flattened fragments less than .2 inches (5 millimeters) in greatest dimension. However, large gold nuggets have been found on Wade Creek; examples are ones of 25,33,56, and 70 ounces. Occasionally, large nuggets may still be found in the tailing piles along the creek. The Fortymile River and its tributaries the South Fork, Walker Fork, and Mosquito Fork, all of which at one time were the sites of bucket-line dredge operations, now are almost exclusively mined using floating suction dredges. Unmined gold-bearing gravel is present in the floodplain of the Walker Fork valley below Cherry Creek and in low (about 100 to 130 feet or 30 to 40 meters) terraces along the north side of Walker Fork and east side of Cherry Creek. Considering the locations of where most gold has been found in the South Fork valley both by the older bucket dredges and the modern suction dredges, it seems likely that the tributary drainages of Lost Chicken, Napoleon, Franklin, and Buckskin Creeks have supplied the bulk of the gold to the South Fork valley. A quarter acre (0.10 hectare), 130-foot-thick ( 40 meters) section of the high terrace gravels on the north side of Napoleon Creek was mined for placer gold and yielded values estimated to be $8.50 per cubic yard (or $6.50 per cubic meter) at $350 per troy ounce. The unmined high terrace gravels on the south side of Buckskin Creek contain gold; however, this gravel is only 3 to 6.5 feet (1 to 2 meters) thick. The search for a lode gold source in the Fortymile River region may be in vain, because substantially more gold than has been recovered from the placers can be derived by the gradual erosion of large volumes of source rocks that contain background mean gold amounts. Using Leon's mass balance equation, 5,167 metric tons of gold may exist in the placers of the Fortymile River region, less than 1 percent of the recovered amount of 15.6 tons. The largest gold resource remaining in the Fortymile River region is probably in the high terrace gravels exposed along many of the creeks and rivers. Until there is exploratory drilling or a comprehensive sampling program, the amount of gold in these gravels will remain unknown. Environmental constraints imposed by Federal and State agencies have slowed, but not stopped, placer mining in the Fortymile River area, and a significant gold price rise would result in more mining.

Integrated Microreactor for Chemical and Biochemical Applications

NASA Technical Reports Server (NTRS)

Schwesinger, N.; Dressler, L.; Frank, Th.; Wurmus, H.

1995-01-01

A completely integrated microreactor was developed that allows for the processing of very small amounts of chemical solutions. The entire system comprises several pumps and valves arranged in different branches as well as a mixing unit and a reaction chamber. The streaming path of each branch contains two valves and one pump each. The pumps are driven by piezoelectric elements mounted on thin glass membranes. Each pump is about 3.5 mm x 3.5 mm x 0.7 mm. A pumping rate up to 25 microliters per hour can be achieved. The operational voltage ranges between 40 and 200 V. A volume stroke up to 1.5 millimeter is achievable from the membrane structures. The valves are designed as passive valves. Sealing is by thin metal films. The dimension of a valve unit is 0.8 x 0.8. 07 mm. The ends of the separate streaming branches are arranged to meet in one point. This point acts as the beginning of a mixer unit which contains several fork-shaped channels. The arrangement of these channels allows for the division of the whole liquid stream into partial streams and their reuniting. A homogeneous mixing of solutions and/or gases can be observed after having passed about 10 of the fork elements. A reaction chamber is arranged behind the mixing unit to support the chemical reaction of special fluids. This unit contains heating elements placed outside of the chamber. The complete system is arranged in a modular structure and is built up of silicon. It comprises three silicon wafers bonded together by applying the silicon direct bonding technology. The silicon structures are made only by wet chemical etching processes. The fluid connections to the outside are realized using standard injection needles glued into v-shaped structures on the silicon wafers. It is possible to integrate other components, like sensors or electronic circuits using silicon as the basic material.

Rinne test: does the tuning fork position affect the sound amplitude at the ear?

PubMed

Butskiy, Oleksandr; Ng, Denny; Hodgson, Murray; Nunez, Desmond A

2016-03-24

Guidelines and text-book descriptions of the Rinne test advise orienting the tuning fork tines in parallel with the longitudinal axis of the external auditory canal (EAC), presumably to maximise the amplitude of the air conducted sound signal at the ear. Whether the orientation of the tuning fork tines affects the amplitude of the sound signal at the ear in clinical practice has not been previously reported. The present study had two goals: determine if (1) there is clinician variability in tuning fork placement when presenting the air-conduction stimulus during the Rinne test; (2) the orientation of the tuning fork tines, parallel versus perpendicular to the EAC, affects the sound amplitude at the ear. To assess the variability in performing the Rinne test, the Canadian Society of Otolaryngology - Head and Neck Surgery members were surveyed. The amplitudes of the sound delivered to the tympanic membrane with the activated tuning fork tines held in parallel, and perpendicular to, the longitudinal axis of the EAC were measured using a Knowles Electronics Mannequin for Acoustic Research (KEMAR) with the microphone of a sound level meter inserted in the pinna insert. 47.4 and 44.8% of 116 survey responders reported placing the fork parallel and perpendicular to the EAC respectively. The sound intensity (sound-pressure level) recorded at the tympanic membrane with the 512 Hz tuning fork tines in parallel with as opposed to perpendicular to the EAC was louder by 2.5 dB (95% CI: 1.35, 3.65 dB; p < 0.0001) for the fundamental frequency (512 Hz), and by 4.94 dB (95% CI: 3.10, 6.78 dB; p < 0.0001) and 3.70 dB (95% CI: 1.62, 5.78 dB; p = .001) for the two harmonic (non-fundamental) frequencies (1 and 3.15 kHz), respectively. The 256 Hz tuning fork in parallel with the EAC as opposed to perpendicular to was louder by 0.83 dB (95% CI: -0.26, 1.93 dB; p = 0.14) for the fundamental frequency (256 Hz), and by 4.28 dB (95% CI: 2.65, 5.90 dB; p < 0.001) and 1.93 dB (95% CI: 0.26, 3.61 dB; p = .02) for the two harmonic frequencies (500 and 4 kHz) respectively. Clinicians vary in their orientation of the tuning fork tines in relation to the EAC when performing the Rinne test. Placement of the tuning fork tines in parallel as opposed to perpendicular to the EAC results in a higher sound amplitude at the level of the tympanic membrane.

Geochemical map of the North Fork John Day River Roadless Area, Grant County, Oregon

USGS Publications Warehouse

Evans, James G.

1986-01-01

The North Fork John Day River Roadless Area comprised 21,210 acres in the Umatilla and Wallowa-Whitman National Forests, Grant County, Oregon, about 30 miles northwest of Baker, Oregon. The irregularly shaped area extends for about 1 mile on both sides of a 25-mile segment of the North Fork John Day River from Big Creek on the west to North Fork John Day Campground on the east. Most of the roadless area is in the northern half of the Desolation Butte 15-minute quadrangle. The eastern end of the area is in parts of the Granite and Trout Meadows 7½-minute quadrangles.

Cancer therapy and replication stress: forks on the road to perdition.

PubMed

Kotsantis, Panagiotis; Jones, Rebecca M; Higgs, Martin R; Petermann, Eva

2015-01-01

Deregulated DNA replication occurs in cancer where it contributes to genomic instability. This process is a target of cytotoxic therapies. Chemotherapies exploit high DNA replication in cancer cells by modifying the DNA template or by inhibiting vital enzymatic activities that lead to slowing or stalling replication fork progression. Stalled replication forks can be converted into toxic DNA double-strand breaks resulting in cell death, i.e., replication stress. While likely crucial for many cancer treatments, replication stress is poorly understood due to its complexity. While we still know relatively little about the role of replication stress in cancer therapy, technical advances in recent years have shed new light on the effect that cancer therapeutics have on replication forks and the molecular mechanisms that lead from obstructed fork progression to cell death. This chapter will give an overview of our current understanding of replication stress in the context of cancer therapy. © 2015 Elsevier Inc. All rights reserved.

Assembly of Slx4 signaling complexes behind DNA replication forks.

PubMed

Balint, Attila; Kim, TaeHyung; Gallo, David; Cussiol, Jose Renato; Bastos de Oliveira, Francisco M; Yimit, Askar; Ou, Jiongwen; Nakato, Ryuichiro; Gurevich, Alexey; Shirahige, Katsuhiko; Smolka, Marcus B; Zhang, Zhaolei; Brown, Grant W

2015-08-13

Obstructions to replication fork progression, referred to collectively as DNA replication stress, challenge genome stability. In Saccharomyces cerevisiae, cells lacking RTT107 or SLX4 show genome instability and sensitivity to DNA replication stress and are defective in the completion of DNA replication during recovery from replication stress. We demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. Slx4 complex formation is nucleated by Mec1 phosphorylation of histone H2A, which is recognized by the constitutive Slx4 binding partner Rtt107. Slx4 is essential for recruiting the Mec1 activator Dpb11 behind stressed replication forks, and Slx4 complexes are important for full activity of Mec1. We propose that Slx4 complexes promote robust checkpoint signaling by Mec1 by stably recruiting Dpb11 within a discrete domain behind the replication fork, during DNA replication stress. © 2015 The Authors.

Using Caffeine as a Water Quality Indicator in the Ambient Monitoring Program for Third Fork Creek Watershed, Durham, North Carolina

PubMed Central

Spence, Porché L

2015-01-01

Caffeine has been suggested as a chemical indicator for domestic wastewater in freshwater systems, although it is not included in water quality monitoring programs. The Third Fork Creek watershed in Durham, NC, is highly urbanized, with a history of receiving untreated wastewater from leaking and overflowing sanitary sewers. The poor water quality originating in the Third Fork Creek watershed threatens its intended uses and jeopardizes drinking water, aquatic life, and recreational activities provided by Jordan Lake. Organic waste contaminants have been detected in both Third Fork Creek watershed and Jordan Lake; however, the sampling periods were temporary, resulting in a few samples collected during nonstorm periods. It is recommended that (1) the concentration of caffeine and other organic waste contaminants are determined during storm and nonstorm periods and (2) caffeine is monitored regularly with traditional water quality indicators to evaluate the health of Third Fork Creek watershed. PMID:26157335

Ecological condition of the East Fork of the Gila River and selected tributaries: Gila National Forest, New Mexico

Treesearch

Robert D. Ohmart

1996-01-01

Ecological condition of riparian habitats along the East Fork of the Gila River, Main Diamond Creek, lower South Diamond Creek, and Black Canyon Creek are all in very heavily degraded condition. Channel cross-sections show extensive entrenchment, high width-to-depth ratios, and numerous reaches where banks are sloughing into the stream, especially on the East Fork of...

The Sound Field around a Tuning Fork and the Role of a Resonance Box

ERIC Educational Resources Information Center

Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

2015-01-01

Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: (1) Why is a bare tuning fork such a weak emitter of sound? (2) What is the role of the…

Qualitative Assessment: Evaluating the Impacts of Climate ...

EPA Pesticide Factsheets

The South Fork Nooksack River (South Fork) is located in northwest Washington State and is home to nine species of Pacific salmon, including Nooksack early Chinook (aka, spring Chinook salmon), an iconic species for the Nooksack Indian Tribe. The quantity of salmon in the South Fork, especially spring Chinook salmon, has dramatically declined from historic levels, due primarily to habitat degradation from the legacy impacts of various land uses such as commercial forestry, agriculture, flood control, and transportation infrastructure. Segments of the South Fork and some of its tributaries exceed temperature criteria established for the protection of cold-water salmonid populations, and were listed on Washington State’s Clean Water Act (CWA) 303(d) list of impaired waterbodies. High water temperatures in the South Fork are detrimental to fish and other native species that depend on cool, clean, well-oxygenated water. Of the nine salmon species, three have been listed as threatened under the federal Endangered Species Act (ESA) and are of high priority to restoration efforts in the South Fork—spring Chinook salmon, summer steelhead trout, and bull trout. Growing evidence shows that climate change will exacerbate legacy impacts. This qualitative assessment is a comprehensive analysis of climate change impacts on freshwater habitat and Pacific salmon in the South Fork. It also evaluates the effectiveness of restoration tools that address Pacific salmon recovery.

The PriA Replication Restart Protein Blocks Replicase Access Prior to Helicase Assembly and Directs Template Specificity through Its ATPase Activity*

PubMed Central

Manhart, Carol M.; McHenry, Charles S.

2013-01-01

The PriA protein serves as an initiator for the restart of DNA replication on stalled replication forks and as a checkpoint protein that prevents the replicase from advancing in a strand displacement reaction on forks that do not contain a functional replicative helicase. We have developed a primosomal protein-dependent fluorescence resonance energy transfer (FRET) assay using a minimal fork substrate composed of synthetic oligonucleotides. We demonstrate that a self-loading reaction, which proceeds at high helicase concentrations, occurs by threading of a preassembled helicase over free 5′-ends, an event that can be blocked by attaching a steric block to the 5′-end or coating DNA with single-stranded DNA binding protein. The specificity of PriA for replication forks is regulated by its intrinsic ATPase. ATPase-defective PriA K230R shows a strong preference for substrates that contain no gap between the leading strand and the duplex portion of the fork, as demonstrated previously. Wild-type PriA prefers substrates with larger gaps, showing maximal activity on substrates on which PriA K230R is inactive. We demonstrate that PriA blocks replicase function on forks by blocking its binding. PMID:23264623

Quartz tuning-fork oscillations in He II and drag coefficient

NASA Astrophysics Data System (ADS)

Gritsenko, I. A.; Zadorozhko, A. A.; Neoneta, A. S.; Chagovets, V. K.; Sheshin, G. A.

2011-07-01

The temperature dependencies of drag coefficient for quartz tuning forks of various geometric dimensions, immersed in the He II, were determined experimentally in the temperature range 0.1-3 K. It is identified, that these dependencies are similar, but the values of drag coefficient are different for tuning forks with different geometric dimensions. It is shown, that the obtained specific drag coefficient depends only on the temperature and frequency of vibrations, when the value of drag coefficient is normalized to the surface area of moving tuning-fork prong. The temperature dependencies of normalized drag coefficient for the tuning forks of various dimensions, wire, and microsphere, oscillating in the Не II, are compared. It is shown, that in the ballistic regime of scattering of quasiparticles, these dependencies are identical and have a slope proportional to T4, which is determined by the density of thermal excitations. In the hydrodynamic regime at T > 0.5 K, the behavior of the temperature dependence of specific drag coefficient is affected by the size and frequency of vibrating body. The empirical relation, which allows to describe the behavior of specific drag coefficient for vibrating tuning forks, microsphere, and wire everywhere over the temperature region and at various frequencies, is proposed.

Movements of adult chinook salmon during spawning migration in a metals-contaminated system, Coeur d'Alene River, Idaho

USGS Publications Warehouse

Goldstein, J.N.; Woodward, D.F.; Farag, A.M.

1999-01-01

Spawning migration of adult male chinook salmon Oncorhynchus tshawytscha was monitored by radio telemetry to determine their response to the presence of metals contamination in the South Fork of the Coeur d'Alene River, Idaho. The North Fork of the Coeur d'Alene River is relatively free of metals contamination and was used as a control. In all, 45 chinook salmon were transported from their natal stream, Wolf Lodge Creek, tagged with radio transmitters, and released in the Coeur d'Alene River 2 km downstream of the confluence of the South Fork and the North Fork of the Coeur d'Alene River. Fixed telemetry receivers were used to monitor the upstream movement of the tagged chinook salmon through the confluence area for 3 weeks after release. During this period, general water quality and metals concentrations were monitored in the study area. Of the 23 chinook salmon observed to move upstream from the release site and through the confluence area, the majority (16 fish, 70%) moved up the North Fork, and only 7 fish (30%) moved up the South Fork, where greater metals concentrations were observed. Our results agree with laboratory findings and suggest that natural fish populations will avoid tributaries with high metals contamination.

Simultaneous binding to the tracking strand, displaced strand and the duplex of a DNA fork enhances unwinding by Dda helicase

PubMed Central

Aarattuthodiyil, Suja; Byrd, Alicia K.; Raney, Kevin D.

2014-01-01

Interactions between helicases and the tracking strand of a DNA substrate are well-characterized; however, the role of the displaced strand is a less understood characteristic of DNA unwinding. Dda helicase exhibited greater processivity when unwinding a DNA fork compared to a ss/ds DNA junction substrate. The lag phase in the unwinding progress curve was reduced for the forked DNA compared to the ss/ds junction. Fewer kinetic steps were required to unwind the fork compared to the ss/ds junction, suggesting that binding to the fork leads to disruption of the duplex. DNA footprinting confirmed that interaction of Dda with a fork leads to two base pairs being disrupted whereas no disruption of base pairing was observed with the ss/ds junction. Neutralization of the phosphodiester backbone resulted in a DNA-footprinting pattern similar to that observed with the ss/ds junction, consistent with disruption of the interaction between Dda and the displaced strand. Several basic residues in the 1A domain which were previously proposed to bind to the incoming duplex DNA were replaced with alanines, resulting in apparent loss of interaction with the duplex. Taken together, these results suggest that Dda interaction with the tracking strand, displaced strand and duplex coordinates DNA unwinding. PMID:25249618

Occurrence and distribution of fecal indicator bacteria, and physical and chemical indicators of water quality in streams receiving discharge from Dallas/Fort Worth International Airport and vicinity, North-Central Texas, 2008

USGS Publications Warehouse

Harwell, Glenn R.; Mobley, Craig A.

2009-01-01

This report, done by the U.S. Geological Survey in cooperation with Dallas/Fort Worth International (DFW) Airport in 2008, describes the occurrence and distribution of fecal indicator bacteria (fecal coliform and Escherichia [E.] coli), and the physical and chemical indicators of water quality (relative to Texas Surface Water Quality Standards), in streams receiving discharge from DFW Airport and vicinity. At sampling sites in the lower West Fork Trinity River watershed during low-flow conditions, geometric mean E. coli counts for five of the eight West Fork Trinity River watershed sampling sites exceeded the Texas Commission on Environmental Quality E. coli criterion, thus not fully supporting contact recreation. Two of the five sites with geometric means that exceeded the contact recreation criterion are airport discharge sites, which here means that the major fraction of discharge at those sites is from DFW Airport. At sampling sites in the Elm Fork Trinity River watershed during low-flow conditions, geometric mean E. coli counts exceeded the geometric mean contact recreation criterion for seven (four airport, three non-airport) of 13 sampling sites. Under low-flow conditions in the lower West Fork Trinity River watershed, E. coli counts for airport discharge sites were significantly different from (lower than) E. coli counts for non-airport sites. Under low-flow conditions in the Elm Fork Trinity River watershed, there was no significant difference between E. coli counts for airport sites and non-airport sites. During stormflow conditions, fecal indicator bacteria counts at the most downstream (integrator) sites in each watershed were considerably higher than counts at those two sites during low-flow conditions. When stormflow sample counts are included with low-flow sample counts to compute a geometric mean for each site, classification changes from fully supporting to not fully supporting contact recreation on the basis of the geometric mean contact recreation criterion. All water temperature measurements at sampling sites in the lower West Fork Trinity River watershed were less than the maximum criterion for water temperature for the lower West Fork Trinity segment. Of the measurements at sampling sites in the Elm Fork Trinity River watershed, 95 percent were less than the maximum criterion for water temperature for the Elm Fork Trinity River segment. All dissolved oxygen concentrations were greater than the minimum criterion for stream segments classified as exceptional aquatic life use. Nearly all pH measurements were within the pH criterion range for the classified segments in both watersheds, except for those at one airport site. For sampling sites in the lower West Fork Trinity River watershed, all annual average dissolved solids concentrations were less than the maximum criterion for the lower West Fork Trinity segment. For sampling sites in the Elm Fork Trinity River, nine of the 13 sites (six airport, three non-airport) had annual averages that exceeded the maximum criterion for that segment. For ammonia, 23 samples from 12 different sites had concentrations that exceeded the screening level for ammonia. Of these 12 sites, only one non-airport site had more than the required number of exceedances to indicate a screening level concern. Stormflow total suspended solids concentrations were significantly higher than low-flow concentrations at the two integrator sites. For sampling sites in the lower West Fork Trinity River watershed, all annual average chloride concentrations were less than the maximum annual average chloride concentration criterion for that segment. For the 13 sampling sites in the Elm Fork Trinity River watershed, one non-airport site had an annual average concentration that exceeded the maximum annual average chloride concentration criterion for that segment.

Geohydrologic Investigations and Landscape Characteristics of Areas Contributing Water to Springs, the Current River, and Jacks Fork, Ozark National Scenic Riverways, Missouri

USGS Publications Warehouse

Mugel, Douglas N.; Richards, Joseph M.; Schumacher, John G.

2009-01-01

The Ozark National Scenic Riverways (ONSR) is a narrow corridor that stretches for approximately 134 miles along the Current River and Jacks Fork in southern Missouri. Most of the water flowing in the Current River and Jacks Fork is discharged to the rivers from springs within the ONSR, and most of the recharge area of these springs is outside the ONSR. This report describes geohydrologic investigations and landscape characteristics of areas contributing water to springs and the Current River and Jacks Fork in the ONSR. The potentiometric-surface map of the study area for 2000-07 shows that the groundwater divide extends beyond the surface-water divide in some places, notably along Logan Creek and the northeastern part of the study area, indicating interbasin transfer of groundwater between surface-water basins. A low hydraulic gradient occurs in much of the upland area west of the Current River associated with areas of high sinkhole density, which indicates the presence of a network of subsurface karst conduits. The results of a low base-flow seepage run indicate that most of the discharge in the Current River and Jacks Fork was from identified springs, and a smaller amount was from tributaries whose discharge probably originated as spring discharge, or from springs or diffuse groundwater discharge in the streambed. Results of a temperature profile conducted on an 85-mile reach of the Current River indicate that the lowest average temperatures were within or downstream from inflows of springs. A mass-balance on heat calculation of the discharge of Bass Rock Spring, a previously undescribed spring, resulted in an estimated discharge of 34.1 cubic feet per second (ft3/s), making it the sixth largest spring in the Current River Basin. The 13 springs in the study area for which recharge areas have been estimated accounted for 82 percent (867 ft3/s of 1,060 ft3/s) of the discharge of the Current River at Big Spring during the 2006 seepage run. Including discharge from other springs, the cumulative discharge from springs was over 90 percent of the river discharge at most of the spring locations, and was 92 percent at Big Spring and at the lower end of the ONSR. The discharge from the 1.9-mile long Pulltite Springs Complex measured in the 2006 seepage run was 88 ft3/s. Most of this (77 ft3/s) was from the first approximately 0.25 mi of the Pulltite Springs Complex. It has been estimated that the annual mean discharge from the Current River Springs Complex is 125 ft3/s, based on an apparent discharge of 50 ft3/s during a 1966 U.S. Geological Survey seepage run. However, a reinterpretation of the 1966 seepage run data shows that the discharge from the Current River Springs Complex instead was about 12.6 ft3/s, and the annual mean discharge was estimated to be 32 ft3/s, substantially less than 125 ft3/s. The 2006 seepage run showed a gain of only 12 ft3/s from the combined Round Spring and Current River Springs Complex from the mouth of Sinking Creek to 0.7 mi upstream from Root Hollow. The 2006 temperature profile measurements did not indicate any influx of spring discharge throughout the length of the Current River Springs Complex. The spring recharge areas with the largest number of identified sinkholes are Big Spring, Alley Spring, and Welch Spring. The spring recharge areas with the largest number of sinkholes per square mile of recharge area are Alley Spring, Blue Spring (Jacks Fork), Welch Spring, and Round Spring and the Current River Springs Complex. Using the currently known locations of losing streams, the Big Spring recharge area has the largest number of miles of losing stream, and the Bass Rock Spring recharge area has the largest number of miles of losing stream per unit recharge area. The spring recharge areas with the most open land and the least forested land per unit recharge area are Blue Spring (Jacks Fork), Welch Spring, Montauk Springs, and Alley Spring. The spring recharge areas with the least amount

Pre- and postharvest preventive measures and intervention strategies to control microbial food safety hazards of fresh leafy vegetables.

PubMed

Gil, Maria I; Selma, Maria V; Suslow, Trevor; Jacxsens, Liesbeth; Uyttendaele, Mieke; Allende, Ana

2015-01-01

This review includes an overview of the most important preventive measures along the farm to fork chain to prevent microbial contamination of leafy greens. It also includes the technological and managerial interventions related to primary production, postharvest handling, processing practices, distribution, and consumer handling to eliminate pathogens in leafy greens. When the microbiological risk is already present, preventive measures to limit actual contamination events or pathogen survival are considered intervention strategies. In codes of practice the focus is mainly put on explaining preventive measures. However, it is also important to establish more focused intervention strategies. This review is centered mainly on leafy vegetables as the commodity identified as the highest priority in terms of fresh produce microbial safety from a global perspective. There is no unique preventive measure or intervention strategy that could be applied at one point of the food chain. We should encourage growers of leafy greens to establish procedures based on the HACCP principles at the level of primary production. The traceability of leafy vegetables along the chain is an essential element in ensuring food safety. Thus, in dealing with the food safety issues associated with fresh produce it is clear that a multidisciplinary farm to fork strategy is required.

Archaeological Investigations in the Halls-Fowlkes Region South Fork of the Forked Deer River, West Tennessee

DTIC Science & Technology

1985-01-01

Obion-Forked Deer River and Reelfoot -Indian Creek drainages (Smith 1979a), the Mud Creek drainage (Dye 1975), the Cypress Creek drainages (Peterson 1975...sites have been identified by the presence of Palmer, Cypress Creek, Lost Lake , Decatur, Kirk Stemmed, Big Sandy, Plevna, Haywood, Kirk Corner Notched...necessary to clarify this problem. Several different Mississippian phases, including the Walls, Boxtown, Ensley, Tiptonville and Reelfoot phases have

Study on vacuum packaging reliability of micromachined quartz tuning fork gyroscopes

NASA Astrophysics Data System (ADS)

Fan, Maoyan; Zhang, Lifang

2017-09-01

Packaging technology of the micromachined quartz tuning fork gyroscopes by vacuum welding has been experimentally studied. The performance of quartz tuning fork is influenced by the encapsulation shell, encapsulation method and fixation of forks. Alloy solder thick film is widely used in the package to avoid the damage of the chip structure by the heat resistance and hot temperature, and this can improve the device performance and welding reliability. The results show that the bases and the lids plated with gold and nickel can significantly improve the airtightness and reliability of the vacuum package. Vacuum packaging is an effective method to reduce the vibration damping, improve the quality factor and further enhance the performance. The threshold can be improved nearly by 10 times.

Maps showing mines, quarries, prospects, and exposures in the Devils Fork Roadless Area, Scott County, Virginia

USGS Publications Warehouse

Behum, Paul T.

1984-01-01

The Devils Fork Roadless Area is located at the eastern edge of the Appalachian coal region and is within the Cumberland Mountain section of the Appalachian Plateau physiographic province. Most of the area is drained by Devil Fork and its tributaries. Clinch Rock Branch of Straight Creek, Roddy Branch of Valley Creek, and Stinking Creek, all tributary to the Clinch River, drain small fringe tracts. Altitudes range from about 1,550 ft on the lower part of Straight Fork to about 3,490 ft at Cox Place on Little Mountain. Vegetation varies from mixed hardwoods in the uplands to thickets of conifer, rhododendron, and laurel in moist protected areas, as in coves along drainage courses.

Replication Fork Protection Factors Controlling R-Loop Bypass and Suppression.

PubMed

Chang, Emily Yun-Chia; Stirling, Peter C

2017-01-14

Replication-transcription conflicts have been a well-studied source of genome instability for many years and have frequently been linked to defects in RNA processing. However, recent characterization of replication fork-associated proteins has revealed that defects in fork protection can directly or indirectly stabilize R-loop structures in the genome and promote transcription-replication conflicts that lead to genome instability. Defects in essential DNA replication-associated activities like topoisomerase, or the minichromosome maintenance (MCM) helicase complex, as well as fork-associated protection factors like the Fanconi anemia pathway, both appear to mitigate transcription-replication conflicts. Here, we will highlight recent advances that support the concept that normal and robust replisome function itself is a key component of mitigating R-loop coupled genome instability.

Crystallization and preliminary X-ray characterization of the eukaryotic replication terminator Reb1-Ter DNA complex.

PubMed

Jaiswal, Rahul; Singh, Samarendra K; Bastia, Deepak; Escalante, Carlos R

2015-04-01

The Reb1 protein from Schizosaccharomyces pombe is a member of a family of proteins that control programmed replication termination and/or transcription termination in eukaryotic cells. These events occur at naturally occurring replication fork barriers (RFBs), where Reb1 binds to termination (Ter) DNA sites and coordinates the polar arrest of replication forks and transcription approaching in opposite directions. The Reb1 DNA-binding and replication-termination domain was expressed in Escherichia coli, purified and crystallized in complex with a 26-mer DNA Ter site. Batch crystallization under oil was required to produce crystals of good quality for data collection. Crystals grew in space group P2₁, with unit-cell parameters a = 68.9, b = 162.9, c = 71.1 Å, β = 94.7°. The crystals diffracted to a resolution of 3.0 Å. The crystals were mosaic and required two or three cycles of annealing. This study is the first to yield structural information about this important family of proteins and will provide insights into the mechanism of replication and transcription termination.

Solar project description for Design Construction Association single family dwelling, Big Fork, Montana

NASA Astrophysics Data System (ADS)

1980-04-01

A solar energy system was installed in a 2100 sq ft house located in Big Fork, Montana. The system is designed to provide solar energy for heating and domestic hot water. Solar energy is collected by flat plate collectors with a gross area of 792 square feet. The collector banks are mounted on the roof of the house and face due south at an angle of 45 deg to the horizontal optimizing solar energy collection. Solar energy is transferred from the collector array to a 1500 gallon storage tank. Water is used as the heat collection, transfer and storage medium. Freeze protection is provided by use of a drain down system. Space heating demands are met by circulating hot water from storage through baseboard units in the distribution system of the house. Auxiliary space heating is provided by an electrical heating element in the boiler. Similarly, an electrical heating element in the DHW tank provides energy for water heating. The dwelling was fully instrumented for performance evaluation since October 1977 and the data is integrated into the National Solar Data Network.

Rotational and accretionary evolution of the Klamath Mountains, California and Oregon, from Devonian to present time

USGS Publications Warehouse

Irwin, William P.; Mankinen, Edward A.

1998-01-01

The purpose of this report is to show graphically how the Klamath Mountains grew from a relatively small nucleus in Early Devonian time to its present size while rotating clockwise approximately 110°. This growth occurred by the addition of large tectonic slices of oceanic lithosphere, volcanic arcs, and melange during a sequence of accretionary episodes. The Klamath Mountains province consists of eight lithotectonoic units called terranes, some of which are divided into subterranes. The Eastern Klamath terrane, which was the early Paleozoic nucleus of the province, is divided into the Yreka, Trinity, and Redding subterranes. Through tectonic plate motion, usually involving subduction, the other terranes joined the early Paleozoic nucleus during seven accretionary episodes ranging in age from Early Devonian to Late Jurassic. The active terrane suture is shown for each episode by a bold black line. Much of the western boundary of the Klamath Mountains is marked by the South Fork and correlative faults along which the Klamath terranes overrode the Coast Range rocks during an eighth accretionary episode, forming the South Fork Mountain Schist in Early Cretaceous time.

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation...

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation...

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation...

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation...

40 CFR 131.34 - Kansas.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Contact Recreation Basin: Solomon Subbasin: Upper North Fork Solomon Ash Creek 10260011 24 Primary Contact... Recreation Subbasin: Lower North Fork Solomon Beaver Creek 10260012 10 Primary Contact Recreation Beaver...

Assessment of possible sources of microbiological contamination in the water column and streambed sediment of the Jacks Fork, Ozark National Scenic Riverways, Missouri - Phase III

USGS Publications Warehouse

Davis, Jerri V.; Barr, Miya N.

2006-01-01

In 1998, a 5 river-mile reach of the Jacks Fork was included on Missouri's list of impaired waters as required by Section 303(d) of the Federal Clean Water Act. The identified pollutant on the Jacks Fork was fecal coliform bacteria. The length of the impaired reach was changed to 7 miles on the Missouri 2002 303(d) list because of data indicating the fecal coliform bacteria problem existed over a broader area. The U.S. Geological Survey, in cooperation with the National Park Service, conducted a study to better understand the extent and sources of microbiological contamination within the Jacks Fork from Alley Spring to the mouth, which includes the 7-mile 303(d) reach. Ten sites were sampled from June 2003 through October 2003 and from June 2004 through October 2004. Water-column and streambed sediment samples were collected from main-stem and tributary sites mostly during base-flow conditions during a variety of recreational season river uses and analyzed for fecal coliform and Escherichia coli bacteria. Isolates of Escherichia coli obtained from water samples collected at five sites were submitted for rep-PCR analysis to identify presumptive sources of fecal indicator bacteria in the Jacks Fork. Results indicate that recreational users (including boaters and swimmers) are not the primary source of fecal coliform bacteria in the Jacks Fork; rather, the presence of fecal coliform bacteria is associated with other animals, of which horses are the primary source. Increases in fecal coliform bacteria densities in the Jacks Fork are associated with cross-country horseback trail-riding events.

Water-Quality, Bed-Sediment, and Biological Data (October 2005 through September 2006) and Statistical Summaries of Long-Term Data for Streams in the Clark Fork Basin, Montana

USGS Publications Warehouse

Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

2007-01-01

Water, bed sediment, and biota were sampled in streams from Butte to below Milltown Reservoir as part of a long-term monitoring program in the upper Clark Fork basin; additional water-quality samples were collected in the Clark Fork basin from sites near Milltown Reservoir downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water-quality samples were collected periodically at 22 sites from October 2005 through September 2006. Bed-sediment and biological samples were collected once at 12 sites during August 2006. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2005 through September 2006. Water-quality data include concentrations of selected major ions, trace ele-ments, and suspended sediment. Nutrients also were analyzed in the supplemental water-quality samples. Daily values of suspended-sed-iment concentration and suspended-sediment discharge were determined for four sites, and seasonal daily values of turbidity were determined for four sites. Bed-sediment data include trace-ele-ment concentrations in the fine-grained fraction. Bio-logical data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

Stream-Sediment Geochemistry in Mining-Impacted Drainages of the Yankee Fork of the Salmon River, Custer County, Idaho

USGS Publications Warehouse

Frost, Thomas P.; Box, Stephen E.

2009-01-01

This reconnaissance study was undertaken at the request of the USDA Forest Service, Region 4, to assess the geochemistry, in particular the mercury and selenium contents, of mining-impacted sediments in the Yankee Fork of the Salmon River in Custer County Idaho. The Yankee Fork has been the site of hard-rock and placer mining, primarily for gold and silver, starting in the 1880s. Major dredge placer mining from the 1930s to 1950s in the Yankee Fork disturbed about a 10-kilometer reach. Mercury was commonly used in early hard-rock mining and placer operations for amalgamation and recovery of gold. During the late 1970s, feasibility studies were done on cyanide-heap leach recovery of gold from low-grade ores of the Sunbeam and related deposits. In the mid-1990s a major open-pit bulk-vat leach operation was started at the Grouse Creek Mine. This operation shut down when gold values proved to be lower than expected. Mercury in stream sediments in the Yankee Fork ranges from below 0.02 ppm to 7 ppm, with the highest values associated with old mill locations and lode and placer mines. Selenium ranges from below the detection limit for this study of 0.2 ppm to 4 ppm in Yankee Fork sediment samples. The generally elevated selenium content in the sediment samples reflect the generally high selenium contents in the volcanic rocks that underlie the Yankee Fork and the presence of gold and silver selenides in some of the veins that were exploited in the early phases of mining.

Shallow stratigraphy of the Skagit River Delta, Washington, derived from sediment cores

USGS Publications Warehouse

Grossman, Eric E.; George, Douglas A.; Lam, Angela

2011-01-01

Sedimentologic analyses of 21 sediment cores, ranging from 0.4 to 9.6 m in length, reveal that the shallow geologic framework of the Skagit River Delta, western Washington, United States, has changed significantly since 1850. The cores collected from elevations of 3.94 to -2.41 m (relative to mean lower low water) along four cross-shore transects between the emergent marsh and delta front show relatively similar environmental changes across an area spanning ~75 km2. Offshore of the present North Fork Skagit River and South Fork Skagit River mouths where river discharge is focused by diked channels through the delta, the entire 5–7-km-wide tidal flats are covered with 1–2 m of cross-bedded medium-to-coarse sands. The bottoms of cores, collected in these areas are composed of mud. A sharp transition from mud to a cross-bedded sand unit indicates that the tidal flats changed abruptly from a calm environment to an energetic one. This is in stark contrast to the Martha's Bay tidal flats north of the Skagit Bay jetty that was completed in the 1940s to protect the newly constructed Swinomish Channel from flooding and sedimentation. North of the jetty, mud ranging from 1 to 2 m thick drapes a previously silt- and sand-rich tidal flat. The silty sand is a sediment facies that would be expected there where North Fork Skagit River sedimentation occurred prior to jetty emplacement. This report describes the compositional and textural properties of the sediment cores by using geophysical, photographic, x-radiography, and standard sediment grain-size and carbon-analytical methods. The findings help to characterize benthic habitat structure and sediment transport processes and the environmental changes that have occurred across the nearshore of the Skagit River Delta. The findings will be useful for quantifying changes to nearshore marine resources, including impacts resulting from diking, river-delta channelization, shoreline development, and natural variations in fluvial-sediment inputs. These results also provide important quantitative data on the amount of sediment delivered to the nearshore from the Skagit River for use in calculating sediment budgets for application to watershed planning and wetland and coastal-ecosystem restoration.

Surface-water quality assessment of the North Fork Red River basin upstream from Lake Altus, Oklahoma, 2002

USGS Publications Warehouse

Smith, S. Jerrod; Schneider, M.L.; Masoner, J.R.; Blazs, R.L.

2003-01-01

Elevated salinity in the North Fork Red River is a major concern of the Bureau of Reclamation W. C. Austin Project at Lake Altus. Understanding the relation between surface-water runoff, ground-water discharge, and surface-water quality is important for maintaining the beneficial use of water in the North Fork Red River basin. Agricultural practices, petroleum production, and natural dissolution of salt-bearing bedrock have the potential to influence the quality of nearby surface water. The U.S. Geological Survey, in cooperation with the Bureau of Reclamation, sampled stream discharge and water chemistry at 19 stations on the North Fork Red River and tributaries. To characterize surface-water resources of the basin in a systematic manner, samples were collected synoptically during receding streamflow conditions during July 8-11, 2002. Together, sulfate and chloride usually constitute greater than half of the dissolved solids. Concentrations of sulfate ranged from 87.1 to 3,450 milligrams per liter. The minimum value was measured at McClellan Creek near Back (07301220), and the maximum value was measured at Bronco Creek near Twitty (07301303). Concentrations of chloride ranged from 33.2 to 786 milligrams per liter. The minimum value was measured at a North Fork Red River tributary (unnamed) near Twitty (07301310), and the maximum value was measured at the North Fork Red River near Back (07301190), the most upstream sample station.

Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.

PubMed

Parajuli, Shankar; Teasley, Daniel C; Murali, Bhavna; Jackson, Jessica; Vindigni, Alessandro; Stewart, Sheila A

2017-09-15

Faithful DNA replication is essential for genome stability. To ensure accurate replication, numerous complex and redundant replication and repair mechanisms function in tandem with the core replication proteins to ensure DNA replication continues even when replication challenges are present that could impede progression of the replication fork. A unique topological challenge to the replication machinery is posed by RNA-DNA hybrids, commonly referred to as R-loops. Although R-loops play important roles in gene expression and recombination at immunoglobulin sites, their persistence is thought to interfere with DNA replication by slowing or impeding replication fork progression. Therefore, it is of interest to identify DNA-associated enzymes that help resolve replication-impeding R-loops. Here, using DNA fiber analysis, we demonstrate that human ribonuclease H1 (RNH1) plays an important role in replication fork movement in the mammalian nucleus by resolving R-loops. We found that RNH1 depletion results in accumulation of RNA-DNA hybrids, slowing of replication forks, and increased DNA damage. Our data uncovered a role for RNH1 in global DNA replication in the mammalian nucleus. Because accumulation of RNA-DNA hybrids is linked to various human cancers and neurodegenerative disorders, our study raises the possibility that replication fork progression might be impeded, adding to increased genomic instability and contributing to disease. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Geologic map of the Dillon quadrangle, Summit and Grand Counties, Colorado

USGS Publications Warehouse

Kellogg, Karl S.

2002-01-01

New 1:24,000-scale geologic mapping along the Interstate-70 urban corridor in western Colorado, in support of the USGS Central Region State/USGS Cooperative Geologic Mapping Project, is contributing to a more complete understanding of the stratigraphy, structure, tectonic evolution, and hazard potential of this rapidly developing region. The 1:24,000-scale Dillon quadrangle is near the headwaters of the Blue River and straddles features of the Blue River graben (Kellogg, 1999), part of the northernmost reaches of the Rio Grande rift, a major late Oligocene to recent zone of extension that extends from Colorado to Mexico. The Williams Range thrust fault, the western structural margin of the Colorado Front Range, cuts through the center of the quadrangle, although is mostly covered by surficial deposits. The oldest rocks in the quadrangle underlie the Williams Fork Mountains and the ridge immediately east of South Fork Middle Fork River, and include biotite-sillimanite schist and gneiss, amphibolite, and migmatite that are intruded by granite inferred to be part of the 1,667-1,750 Ma Routt Plutonic Suite (Tweto, 1987). The oldest exposed sedimentary unit is the Upper Jurassic Morrison Formation, but Pennsylvanian Maroon Formation, a sequence of red sandstone, conglomerate, and interbedded shale, underlies the southern part of the quadrangle. The thickest sequence of sedimentary rocks is Cretaceous in age and includes at least 500 m of the Upper Cretaceous Pierre Shale. Surficial deposits include (1) an old, deeply dissected landslide deposit, possibly as old as Pliocene, on the west flank of the Williams Fork Mountains, (2) deeply weathered, very coarse gravel deposits underlying a mesa in the southwest part of the quadrangle (the Mesa Cortina subdivision. The gravels are gold bearing and were mined by hydraulic methods in the 1800s), (3) moderately to deeply weathered, widespread, bouldery material that is a combination of till of the Bull Lake glaciation, debris-flow deposits, landslide deposits, and possibly pre-Bull Lake till, (4) glacial deposits of both Bull Lake (middle Pleistocene) and Pinedale (late Pleistocene)glaciations, (5) recent landslide deposits, and (6)extensive colluvial and alluvial deposits.

Geologic map of the Dillon quadrangle, Summit and Grand Counties, Colorado

USGS Publications Warehouse

Kellogg, Karl S.

1997-01-01

New 1:24,000-scale geologic mapping along the Interstate-70 urban corridor in western Colorado, in support of the USGS Central Region State/USGS Cooperative Geologic Mapping Project, is contributing to a more complete understanding of the stratigraphy, structure, tectonic evolution, and hazard potential of this rapidly developing region. The 1:24,000-scale Dillon quadrangle is near the headwaters of the Blue River and straddles features of the Blue River graben (Kellogg, 1999), part of the northernmost reaches of the Rio Grande rift, a major late Oligocene to recent zone of extension that extends from Colorado to Mexico. The Williams Range thrust fault, the western structural margin of the Colorado Front Range, cuts through the center of the quadrangle, although is mostly covered by surficial deposits. The oldest rocks in the quadrangle underlie the Williams Fork Mountains and the ridge immediately east of South Fork Middle Fork River, and include biotite-sillimanite schist and gneiss, amphibolite, and migmatite that are intruded by granite inferred to be part of the 1,667-1,750 Ma Routt Plutonic Suite (Tweto, 1987). The oldest exposed sedimentary unit is the Upper Jurassic Morrison Formation, but Pennsylvanian Maroon Formation, a sequence of red sandstone, conglomerate, and interbedded shale, underlies the southern part of the quadrangle. The thickest sequence of sedimentary rocks is Cretaceous in age and includes at least 500 m of the Upper Cretaceous Pierre Shale. Surficial deposits include (1) an old, deeply dissected landslide deposit, possibly as old as Pliocene, on the west flank of the Williams Fork Mountains, (2) deeply weathered, very coarse gravel deposits underlying a mesa in the southwest part of the quadrangle (the Mesa Cortina subdivision. The gravels are gold bearing and were mined by hydraulic methods in the 1800s), (3) moderately to deeply weathered, widespread, bouldery material that is a combination of till of the Bull Lake glaciation, debris-flow deposits, landslide deposits, and possibly pre-Bull Lake till, (4) glacial deposits of both Bull Lake (middle Pleistocene) and Pinedale (late Pleistocene)glaciations, (5) recent landslide deposits, and (6)extensive colluvial and alluvial deposits.

Historical and current perspectives on fish assemblages of the Snake River, Idaho and Wyoming

USGS Publications Warehouse

Maret, T.R.; Mebane, C.A.

2005-01-01

The Snake River is the tenth longest river in the United States, extending 1,667 km from its origin in Yellowstone National Park in western Wyoming to its union with the Columbia River at Pasco, Washington. Historically, the main-stem Snake River upstream from the Hells Canyon Complex supported at least 26 native fish species, including anadromous stocks of Chinook salmon Oncorhynchus tshawytscha, steelhead O. mykiss, Pacific lamprey Lampetra tridentata, and white sturgeon Acipenser transmontanus. Of these anadromous species, only the white sturgeon remains in the Snake River between the Hells Canyon Complex and Shoshone Falls. Today, much of the Snake River has been transformed into a river with numerous impoundments and flow diversions, increased pollutant loads, and elevated water temperatures. Current (1993-2002) fish assemblage collections from 15 sites along the Snake River and Henrys Fork contained 35 fish species, including 16 alien species. Many of these alien species such as catfish (Ictaluridae), carp (Cyprinidae), and sunfish (Centrarchidae) are adapted for warmwater impounded habitats. Currently, the Snake River supports 19 native species. An index of biotic integrity (IBI), developed to evaluate large rivers in the Northwest, was used to evaluate recent (1993-2002) fish collections from the Snake River and Henrys Fork in southern Idaho and western Wyoming. Index of biotic integrity site scores and component metrics revealed a decline in biotic integrity from upstream to downstream in both the Snake River and Henrys Fork. Two distinct groups of sites were evident that correspond to a range of IBI scores-an upper Snake River and Henrys Fork group with relatively high biotic integrity (mean IBI scores of 46-84) and a lower Snake River group with low biotic integrity (mean IBI scores of 10-29). Sites located in the lower Snake River exhibited fish assemblages that reflect poor-quality habitat where coldwater and sensitive species are rare or absent, and where tolerant, less desirable species predominate. Increases in percentages of agricultural land, total number of diversions, and number of constructed channels were strongly associated with these decreasing IBI scores.

Water and Proppant Requirements and Water Production Associated with Undiscovered Petroleum in the Bakken and Three Forks Formations, North Dakota and Montana, USA

NASA Astrophysics Data System (ADS)

Haines, S. S.; Varela, B. A.; Thamke, J.; Hawkins, S. J.; Gianoutsos, N. J.; Tennyson, M. E.

2017-12-01

Water is used for several stages of oil and gas production, in particular for hydraulic fracturing that is typically used during production of petroleum from low-permeability shales and other rock types (referred to as "continuous" petroleum accumulations). Proppant, often sand, is also consumed during hydraulic fracturing. Water is then produced from the reservoir along with the oil and gas, representing either a disposal consideration or a possible source of water for further petroleum development or other purposes. The U.S. Geological Survey (USGS) has developed an approach for regional-scale estimation of these water and proppant quantities in order to provide an improved understanding of possible impacts and to help with planning and decision-making. Using the new methodology, the USGS has conducted a quantitative assessment of water and proppant requirements, and water production volumes, associated with associated with possible future production of undiscovered petroleum resources in the Bakken and Three Forks Formations, Williston Basin, USA. This water and proppant assessment builds directly from the 2013 USGS petroleum assessment for the Bakken and Three Forks Formations. USGS petroleum assessments incorporate all available geologic and petroleum production information, and include the definition of assessment units (AUs) that specify the geographic regions and geologic formations for the assessment. The 2013 petroleum assessment included 5 continuous AUs for the Bakken Formation and one continuous AU for the Three Forks Formation. The assessment inputs are defined probabilistically, and a Monte Carlo approach provides outputs that include uncertainty bounds. We can summarize the assessment outputs with the mean values of the associated distributions. The mean estimated total volume of water for well drilling and cement for all six continuous AUs is 5.9 billion gallons, and the mean estimated volume of water for hydraulic fracturing for all AUs is 164.3 billion gallons. The mean estimated quantity of proppant for hydraulic fracturing is 101.3 million tons. Summing over all of the AUs, the mean estimated total flowback water volume is 9.9 billion gallons and the mean estimated total produced water is 414.5 billion gallons.

Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks.

PubMed

Sotiriou, Sotirios K; Kamileri, Irene; Lugli, Natalia; Evangelou, Konstantinos; Da-Ré, Caterina; Huber, Florian; Padayachy, Laura; Tardy, Sebastien; Nicati, Noemie L; Barriot, Samia; Ochs, Fena; Lukas, Claudia; Lukas, Jiri; Gorgoulis, Vassilis G; Scapozza, Leonardo; Halazonetis, Thanos D

2016-12-15

Human cancers are characterized by the presence of oncogene-induced DNA replication stress (DRS), making them dependent on repair pathways such as break-induced replication (BIR) for damaged DNA replication forks. To better understand BIR, we performed a targeted siRNA screen for genes whose depletion inhibited G1 to S phase progression when oncogenic cyclin E was overexpressed. RAD52, a gene dispensable for normal development in mice, was among the top hits. In cells in which fork collapse was induced by oncogenes or chemicals, the Rad52 protein localized to DRS foci. Depletion of Rad52 by siRNA or knockout of the gene by CRISPR/Cas9 compromised restart of collapsed forks and led to DNA damage in cells experiencing DRS. Furthermore, in cancer-prone, heterozygous APC mutant mice, homozygous deletion of the Rad52 gene suppressed tumor growth and prolonged lifespan. We therefore propose that mammalian RAD52 facilitates repair of collapsed DNA replication forks in cancer cells. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

DNA Replication Origins and Fork Progression at Mammalian Telomeres

PubMed Central

Higa, Mitsunori; Fujita, Masatoshi; Yoshida, Kazumasa

2017-01-01

Telomeres are essential chromosomal regions that prevent critical shortening of linear chromosomes and genomic instability in eukaryotic cells. The bulk of telomeric DNA is replicated by semi-conservative DNA replication in the same way as the rest of the genome. However, recent findings revealed that replication of telomeric repeats is a potential cause of chromosomal instability, because DNA replication through telomeres is challenged by the repetitive telomeric sequences and specific structures that hamper the replication fork. In this review, we summarize current understanding of the mechanisms by which telomeres are faithfully and safely replicated in mammalian cells. Various telomere-associated proteins ensure efficient telomere replication at different steps, such as licensing of replication origins, passage of replication forks, proper fork restart after replication stress, and dissolution of post-replicative structures. In particular, shelterin proteins have central roles in the control of telomere replication. Through physical interactions, accessory proteins are recruited to maintain telomere integrity during DNA replication. Dormant replication origins and/or homology-directed repair may rescue inappropriate fork stalling or collapse that can cause defects in telomere structure and functions. PMID:28350373

Water-quality and algal conditions in the Clackamas River basin, Oregon, and their relations to land and water management

USGS Publications Warehouse

Carpenter, Kurt D.

2003-01-01

In 1998, the U.S. Geological Survey sampled the Clackamas River, its major tributaries, and reservoirs to characterize basic water quality (nutrients, dissolved oxygen, pH, temperature, and conductance), water quantity (water sources within the basin), and algal conditions (biomass and species composition). Sampling locations reflected the dominant land uses in the basin (forest management, agriculture, and urban development) as well as the influence of hydroelectric projects, to examine how these human influences might be affecting water quality and algal conditions. Nuisance algal growths, with accompanying negative effects on water quality, were observed at several locations in the basin during this study. Algal biomass in the lower Clackamas River reached a maximum of 300 mg/m2 chlorophyll a, producing nuisance algal conditions, including fouled stream channels and daily fluctuations in pH and dissolved oxygen concentrations to levels that did not meet water-quality standards. Algal biomass was highest at sites immediately downstream from the hydroelectric project's reservoirs and/or powerhouses. Nuisance algal conditions also were observed in some of the tributaries, including the North Fork of the Clackamas River, Clear Creek, Rock Creek, and Sieben Creek. High amounts of drifting algae increased turbidity levels in the Clackamas River during June, which coincided with a general increase in the concentration of disinfection by-products found in treated Clackamas River water used for drinking, presumably due to the greater amounts of organic matter in the river. The highest nutrient concentrations were found in the four lowermost tributaries (Deep, Richardson, Rock, and Sieben Creeks), where most of the agriculture and urban development is concentrated. Of these, the greatest load of nutrients came from Deep Creek, which had both high nutrient concentrations and relatively high streamflow. Streams draining forestland in the upper basin (upper Clackamas River and Oak Grove Fork) had the highest concentrations of phosphorus (and lowest concentrations of nitrogen), and streams draining forestland in the middle basin (Clear Creek, Eagle Creek, and the North Fork of the Clackamas River) had relatively high concentrations of nitrogen (and low concentrations of phosphorus). In contrast, relatively low concentrations of both nitrogen and phosphorus were found at the two reference streams, reflecting their pristine condition. Relatively high phosphorus levels in the upper basin are probably due to the erosion of naturally occurring phosphorus deposits in this area. Likely sources of nitrogen (mostly nitrate) in the forested watersheds include nitrogen-fixing plants, atmospheric deposition, timber harvesting, and applications of urea fertilizers.

Distinct functions of human RecQ helicases during DNA replication.

PubMed

Urban, Vaclav; Dobrovolna, Jana; Janscak, Pavel

2017-06-01

DNA replication is the most vulnerable process of DNA metabolism in proliferating cells and therefore it is tightly controlled and coordinated with processes that maintain genomic stability. Human RecQ helicases are among the most important factors involved in the maintenance of replication fork integrity, especially under conditions of replication stress. RecQ helicases promote recovery of replication forks being stalled due to different replication roadblocks of either exogenous or endogenous source. They prevent generation of aberrant replication fork structures and replication fork collapse, and are involved in proper checkpoint signaling. The essential role of human RecQ helicases in the genome maintenance during DNA replication is underlined by association of defects in their function with cancer predisposition. Copyright © 2016 Elsevier B.V. All rights reserved.

Comparison of Three Biomass Sampling Techniques on Submersed Aquatic Plants in a Northern Tier Lake

DTIC Science & Technology

2010-07-01

distribution in 3 out of 14 species when comparing the box-core sampler and the rake method. These included forked duckweed (Lemna trisulca L, p...each site did not exhibit differences. These included coontail (p=0.2949), muskgrass (p=0.2746), American elodea (p=0.7622), forked duckweed (p...collected by the PVC-core sampler. These included coontail (p=0.000), chara (p=0.0219), American elodea (p=0.0061), forked duckweed (p=0.0000), najas (p

Installation Restoration Program, Phase 2. Confirmation/Quantification, Stage 1. Kalispell Air Force Station, Montana

DTIC Science & Technology

1989-05-01

AFB Statement of Work. The Phase II Stage 1 investigation of Malmstrom AFB and two off-Base sites at Shelby and Brady, Montana are described in a...Flathead National Forest Headquarters in Kalispell and the Big Fork Ranger Station in Big Fork . Because there has been litigation involved with this...reviewed by phone. An interview at the spill site was held with Mr. William Pedersen, U.S. Forest Service Ranger for the Big Fork District. He personally

ANSYS simulation of the capacitance coupling of quartz tuning fork gyroscope

NASA Astrophysics Data System (ADS)

Zhang, Qing; Feng, Lihui; Zhao, Ke; Cui, Fang; Sun, Yu-nan

2013-12-01

Coupling error is one of the main error sources of the quartz tuning fork gyroscope. The mechanism of capacitance coupling error is analyzed in this article. Finite Element Method (FEM) is used to simulate the structure of the quartz tuning fork by ANSYS software. The voltage output induced by the capacitance coupling is simulated with the harmonic analysis and characteristics of electrical and mechanical parameters influenced by the capacitance coupling between drive electrodes and sense electrodes are discussed with the transient analysis.

An Inventory and Evaluation of Architectural and Engineering Resources of the Big South Fork National River and Recreation Area, Tennessee and Kentucky.

DTIC Science & Technology

1982-02-25

coordinated multidisciplinary study of both the architectural and engineering resources of the National Area. Both research b1 orientation and...South Fork just north of Rugby , and traveled through the site where Jamestown, Tennessee, now stands. A third trail, the Chickamauga Path, left the...Thomas Hughes (1881), the founder of the English colony of Rugby , Tennessee, described his neighbors in the Big South Fork area as mostly poor men

41 CFR 101-25.405 - Materials handling equipment.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 50 45 40 30 25 20 15 10 Straddle truck 15 50 50 50 45 45 45 40 40 35 35 30 25 20 15 10 Electric Fork... percentage of acquisition costs (years in use) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Gasoline Fork truck (2000 pounds to 6000 pounds) 8 50 45 40 30 25 20 15 10 Fork truck (over 6000 pounds) 10 50 45 40 35 30 25 20 15...

Spring constant of a tuning-fork sensor for dynamic force microscopy

PubMed Central

Lange, Manfred; Schmuck, Merlin; Schmidt, Nico; Möller, Rolf

2012-01-01

Summary We present an overview of experimental and numerical methods to determine the spring constant of a quartz tuning fork in qPlus configuration. The simple calculation for a rectangular cantilever is compared to the values obtained by the analysis of the thermal excitation and by the direct mechanical measurement of the force versus displacement. To elucidate the difference, numerical simulations were performed taking account of the real geometry including the glue that is used to mount the tuning fork. PMID:23365793

Structure of the replication fork in ultraviolet light-irradiated human cells.

PubMed Central

Cordeiro-Stone, M; Schumacher, R I; Meneghini, R

1979-01-01

The DNA extracted from xeroderma pigmentosum human fibroblasts previously irradiated with 12.5 J/m2 of UV light and pulse-labeled for 45 min with radioactive and (or) heavy precursors, was used to determine the structural characteristics of the replication fork. Density equilibrium centrifugation experiments showed that a fork moved 6 micrometer in 45 min and bypassed 3 pyrimidine dimers in both strands. The same length was covered in 15-20 min in control cells. The delay in irradiated cells was apparently due to pyrimidine dimers acting as temporary blocks to the fork movement. Evidence for this interpretation comes from kinetics of incorporation of [3H]thymidine into DNA, which show that the time necessary to attain a new stable level of DNA synthesis in irradiated cells is equivalent to that required for the replication fork to cover the interdimer distance in one strand. On the other hand, the action of S1 nuclease on DNA synthesized soon after irradiation gives rise to a bimodal distribution in neutral sucrose gradients, one peak corresponding to 43 X 10(6) daltons and the other to 3 X 10(6) daltons. These two DNA species are generated by the attack of the S1 nuclease on single-stranded regions associated with the replication fork. A possible explanation for these results is given by a model according to which there is a delayed bypass of the dimer in the leading strand and the appearance of gaps opposite pyrimidine dimers in the lagging strand, as a direct consequence of the discontinuous mode of DNA replication. In terms of the model, the DNA of 43 X 10(6) daltons corresponds to the leading strand, linked to the unreplicated branch of the forks, whereas the piece of 3 X 10(6) daltons is the intergap DNA coming from the lagging strand. Pulse and chase experiments reveal that the low molecular weight DNA grows in a pattern that suggests that more than one gap may be formed per replication fork. PMID:233582

Structure of the replication fork in ultraviolet light-irradiated human cells.

PubMed

Cordeiro-Stone, M; Schumacher, R I; Meneghini, R

1979-08-01

The DNA extracted from xeroderma pigmentosum human fibroblasts previously irradiated with 12.5 J/m2 of UV light and pulse-labeled for 45 min with radioactive and (or) heavy precursors, was used to determine the structural characteristics of the replication fork. Density equilibrium centrifugation experiments showed that a fork moved 6 micrometer in 45 min and bypassed 3 pyrimidine dimers in both strands. The same length was covered in 15-20 min in control cells. The delay in irradiated cells was apparently due to pyrimidine dimers acting as temporary blocks to the fork movement. Evidence for this interpretation comes from kinetics of incorporation of [3H]thymidine into DNA, which show that the time necessary to attain a new stable level of DNA synthesis in irradiated cells is equivalent to that required for the replication fork to cover the interdimer distance in one strand. On the other hand, the action of S1 nuclease on DNA synthesized soon after irradiation gives rise to a bimodal distribution in neutral sucrose gradients, one peak corresponding to 43 X 10(6) daltons and the other to 3 X 10(6) daltons. These two DNA species are generated by the attack of the S1 nuclease on single-stranded regions associated with the replication fork. A possible explanation for these results is given by a model according to which there is a delayed bypass of the dimer in the leading strand and the appearance of gaps opposite pyrimidine dimers in the lagging strand, as a direct consequence of the discontinuous mode of DNA replication. In terms of the model, the DNA of 43 X 10(6) daltons corresponds to the leading strand, linked to the unreplicated branch of the forks, whereas the piece of 3 X 10(6) daltons is the intergap DNA coming from the lagging strand. Pulse and chase experiments reveal that the low molecular weight DNA grows in a pattern that suggests that more than one gap may be formed per replication fork.

[History of the tuning fork. III: On the way to quantitative pure-tone measurement. Pictures from the history of otorhinolaryngology, represented by instruments from the collection of the Ingolstadt German Medical History Museum].

PubMed

Feldmann, H

1997-07-01

Weber's and Rinne's tuning-fork tests were for a long time considered unreliable, as they often seemed to yield inconsistent results. The sources of error were manifold and lay in the fields of physics, physiology, pathophysiology, and psychology. When the problems came to be understood, more sophisticated instruments and techniques were developed. The prongs of the tuning fork were fitted with clamps to deaden overtones when it was put into vibration (Politzer 1870). By shifting the clamps along the prongs the tone of the tuning fork could be varied in a range up to one octave (Könlg 1878). A knob of hom or metal was fixed to the end of the shaft to ensure a good coupling to the skull when testing bone conduction (Lucae 1886). A small hammer fixed to the shaft and driven by a spring would activate the tuning fork with reproducible strength (Lucae 1899). A wedge-shaped figure drawn on the lateral surface of the clamps would allow one to optically control the amplitude of vibration (Gradenigo 1899). The time during which a patient hears the tuning fork after it has been struck as compared to that of a normal hearing subject was measured as parameter of hearing acutiy (v. Conta 1864). A number of tuning forks at intervals of one octave each were assembled in sets to cover the whole frequency range of hearing. The most sophisticated example of these sets was the Bezold-Edelmann continuous tone series (1894). It comprised ten tuning forks with sliding clamps, two pipes of the organ type, and a Galton whistle. With this instrumentation it was possible to test the whole range of hearing. The results of testing the hearing via air conduction and bone conduction measured in duration and calculated as percentage of normal hearing were presented in charts (Hartmann 1885, Gradenigo 1893) which can be considered precursors of modern audiograms. The evolution of these instruments and methods is described in detail and illustrated by exhibits from the museum.

Recharge of shallow aquifers through two ephemeral-stream channels in northeastern Wyoming, 1982-1983

USGS Publications Warehouse

Lenfest, L.W.

1987-01-01

Quantifying the recharge from ephemeral streams to alluvial and bedrock aquifers will help evaluate the effects of surface mining on alluvial valley floors in Wyoming. Two stream reaches were chosen for study in the Powder River basin. One reach was located along the North Fork Dry Fork Cheyenne River near Glenrock, Wyoming, and the other reach was located along Black Thunder Creek near Hampshire, Wyoming. The reach along the North Fork Dry Fork Cheyenne River was instrumented with 3 gaging stations to measure streamflow and with 6 observation wells to measure groundwater level fluctuations in alluvial and bedrock aquifers in response to streamflow. The 3 streamflow gaging stations were located within the 2.5-mi study reach to measure the approximate gain or loss of discharge along the reach. Computed streamflow losses ranged from 0.43 acre-ft/mi on July 9 , 1982, to 1.44 acre-ft/mi on August 9, 1982. The observation wells completed only in the alluvial aquifer were dry during flow in the North Fork Dry Fork Cheyenne River, whereas water levels in half of the observation wells completed in the bedrock aquifers or the alluvial and bedrock aquifers rose in response to flow in the North Fork Dry Fork Cheyenne River. Groundwater recharge on August 9, 1982, was calculated using a convolution technique using groundwater levels at the upstream site and was estimated to be 26.5 acre-ft/mi. The reach along Black Thunder Creek was instrumented with one gaging station to measure streamflow and with 4 observation wells to measure water level response in alluvial and bedrock aquifers to streamflow. Recharge to the alluvial aquifer from flow in Black Thunder Creek ranged from 3.56 to 12.4 acre-ft/mi. The recharge was estimated using the convolution technique using water level measurements in the observation wells completed in the alluvial aquifer. Water level measurements in the observation wells indicated water level rises in the alluvial and bedrock aquifers in response to flow in Black Thunder Creek. (Author 's abstract)

14. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM TOWARD SETTLING BASIN, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM TOWARD SETTLING BASIN, SHOWING RIGHT FORK TO BYPASS, LEFT FORK TO BASIN - Electron Hydroelectric Project, Along Puyallup River, Electron, Pierce County, WA

77 FR 41897 - Airworthiness Directives; PZL Swidnik S.A. Helicopters

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-17

... control system hydraulic actuator fork end. These actions are intended to prevent rotation of the... locking of the longitudinal control system hydraulic actuator fork end. This condition, if not detected...

Little Known Facts about the Common Tuning Fork.

ERIC Educational Resources Information Center

Ong, P. P.

2002-01-01

Explains the physical principles of the tuning fork which has a common use in teaching laboratories. Includes information on its vibration, frequency of vibration, elasticity, and reasons for having two prongs. (YDS)

NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection

PubMed Central

Abeyta, Antonio; Castella, Maria; Jacquemont, Celine; Taniguchi, Toshiyasu

2017-01-01

ABSTRACT Proteins essential for homologous recombination play a pivotal role in the repair of DNA double strand breaks, DNA inter-strand crosslinks and replication fork stability. Defects in homologous recombination also play a critical role in the development of cancer and the sensitivity of these cancers to chemotherapy. RAD51, an essential factor for homologous recombination and replication fork protection, accumulates and forms immunocytochemically detectable nuclear foci at sites of DNA damage. To identify kinases that may regulate RAD51 localization to sites of DNA damage, we performed a human kinome siRNA library screen, using DNA damage-induced RAD51 foci formation as readout. We found that NEK8, a NIMA family kinase member, is required for efficient DNA damage-induced RAD51 foci formation. Interestingly, knockout of Nek8 in murine embryonic fibroblasts led to cellular sensitivity to the replication inhibitor, hydroxyurea, and inhibition of the ATR kinase. Furthermore, NEK8 was required for proper replication fork protection following replication stall with hydroxyurea. Loading of RAD51 to chromatin was decreased in NEK8-depleted cells and Nek8-knockout cells. Single-molecule DNA fiber analyses revealed that nascent DNA tracts were degraded in the absence of NEK8 following treatment with hydroxyurea. Consistent with this, Nek8-knockout cells showed increased chromosome breaks following treatment with hydroxyurea. Thus, NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51. PMID:27892797

NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection.

PubMed

Abeyta, Antonio; Castella, Maria; Jacquemont, Celine; Taniguchi, Toshiyasu

2017-02-16

Proteins essential for homologous recombination play a pivotal role in the repair of DNA double strand breaks, DNA inter-strand crosslinks and replication fork stability. Defects in homologous recombination also play a critical role in the development of cancer and the sensitivity of these cancers to chemotherapy. RAD51, an essential factor for homologous recombination and replication fork protection, accumulates and forms immunocytochemically detectable nuclear foci at sites of DNA damage. To identify kinases that may regulate RAD51 localization to sites of DNA damage, we performed a human kinome siRNA library screen, using DNA damage-induced RAD51 foci formation as readout. We found that NEK8, a NIMA family kinase member, is required for efficient DNA damage-induced RAD51 foci formation. Interestingly, knockout of Nek8 in murine embryonic fibroblasts led to cellular sensitivity to the replication inhibitor, hydroxyurea, and inhibition of the ATR kinase. Furthermore, NEK8 was required for proper replication fork protection following replication stall with hydroxyurea. Loading of RAD51 to chromatin was decreased in NEK8-depleted cells and Nek8-knockout cells. Single-molecule DNA fiber analyses revealed that nascent DNA tracts were degraded in the absence of NEK8 following treatment with hydroxyurea. Consistent with this, Nek8-knockout cells showed increased chromosome breaks following treatment with hydroxyurea. Thus, NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51.

Paleomagnetism and rock magnetism of remagnetized carbonate rocks from the Helena Salient, western Montana

NASA Astrophysics Data System (ADS)

Baugh, B.; Housen, B. A.; Burmester, R. F.

2010-12-01

The Helena salient is a curved orogen in southwest Montana, characterized by thin-skinned folding and thrusting. Ages dated from volcanic sills imply that deformation in the region began 77 million years ago during the late Cretaceous (Harlan et al, 1998). This study investigates the nature of curvature associated with this orogen using paleomagnetic techniques. Carbonate rocks of the Mississippian Madison Group were sampled from 24 sites across three folds: the Devil’s Fence anticline, the Three Forks anticline and the Turner anticline (near Townsend, MT). Results from 16 sites have well defined, but very weak, magnetizations. At 100% untilting, two components of magnetization are revealed: a Mississippian primary magnetization (M-group) from at least two sites, and a Late Cretaceous chemical remanent magnetization (CRM) for 13 sites (K-group). Fold tests for the K-group indicate that each fold acquired a magnetization at 90-100% untilting. A mean direction, in geographic coordinates and D = 35°, I = 72.9°, α95 = 8.8° is obtained for Devil’s Fence anticline, D = 37.7°, I = 70.1°, α95 = 23.9° for Three forks anticline and D = 224.6°, I = 69.1°, α95 = 29.1° for Turner anticline. Using a calculated late Cretaceous NA reference pole (D = 335.8, I = 70.1 and ΔDx = 6.3°;), the K-group indicates large (~60° CW) vertical axis rotations from Devil’s Fence and Three Forks anticlines, as well as 111° counter-clockwise rotation from Turner anticline, since the Late Cretaceous. Hysteresis data fall on the SP-PSD mixing lines, consistent with rock magnetic data from other studies sampling remagnetized carbonate units (e.g. Suk et al., 1993; Xu et al., 1998). When K-group directions are un-rotated on an equal-area plot to a Late Cretaceous NA reference pole, the M-group restores nearly to a Mississippian NA reference pole (D = 310, I = 8.2 and ΔDx = 4.7°) and indicates a clockwise rotation of the pre-deformational sedimentary basin of 22 ± 18° to 59 ± 14°. Pink units show Mission Canyon limestone exposures. Yellow units show Lodgepole limestone exposures. Rectangles are locations of the three anticlines sampled in this study. In red are approximate thrust trace locations, which disappear beneath quaternary alluvial deposits. The western most trace is the Lombard thrust fault.

Molecular biology of Hel308 helicase in archaea.

PubMed

Woodman, Isabel L; Bolt, Edward L

2009-02-01

Hel308 is an SF2 (superfamily 2) helicase with clear homologues in metazoans and archaea, but not in fungi or bacteria. Evidence from biochemistry and genetics implicates Hel308 in remodelling compromised replication forks. In the last 4 years, significant advances have been made in understanding the biochemistry of archaeal Hel308, most recently through atomic structures from cren- and eury-archaea. These are good templates for SF2 helicase function more generally, highlighting co-ordinated actions of accessory domains around RecA folds. We review the emerging molecular biology of Hel308, drawing together ideas of how it may contribute to genome stability through the control of recombination, with reference to paradigms developed in bacteria.

Flexible and wearable 3D graphene sensor with 141 KHz frequency signal response capability

NASA Astrophysics Data System (ADS)

Xu, R.; Zhang, H.; Cai, Y.; Ruan, J.; Qu, K.; Liu, E.; Ni, X.; Lu, M.; Dong, X.

2017-09-01

We developed a flexible force sensor consisting of 3D graphene foam (GF) encapsulated in flexible polydimethylsiloxane (PDMS). Because the 3D GF/PDMS sensor is based on the transformation of an electronic band structure aroused by static mechanical strain or KHz vibration, it can detect frequency signals by both tuning fork tests and piezoelectric ceramic transducer tests, which showed a clear linear response from audio frequencies, including frequencies up to 141 KHz in the ultrasound range. Because of their excellent response with a wide bandwidth, the 3D GF/PDMS sensors are attractive for interactive wearable devices or artificial prosthetics capable of perceiving seismic waves, ultrasonic waves, shock waves, and transient pressures.

Water-quality trends and constituent-transport analysis for selected sampling sites in the Milltown Reservoir/Clark Fork River Superfund Site in the upper Clark Fork Basin, Montana, water years 1996–2015

USGS Publications Warehouse

Sando, Steven K.; Vecchia, Aldo V.

2016-07-20

During the extended history of mining in the upper Clark Fork Basin in Montana, large amounts of waste materials enriched with metallic contaminants (cadmium, copper, lead, and zinc) and the metalloid trace element arsenic were generated from mining operations near Butte and milling and smelting operations near Anaconda. Extensive deposition of mining wastes in the Silver Bow Creek and Clark Fork channels and flood plains had substantial effects on water quality. Federal Superfund remediation activities in the upper Clark Fork Basin began in 1983 and have included substantial remediation near Butte and removal of the former Milltown Dam near Missoula. To aid in evaluating the effects of remediation activities on water quality, the U.S. Geological Survey began collecting streamflow and water-quality data in the upper Clark Fork Basin in the 1980s.Trend analysis was done on specific conductance, selected trace elements (arsenic, copper, and zinc), and suspended sediment for seven sampling sites in the Milltown Reservoir/Clark Fork River Superfund Site for water years 1996–2015. The most upstream site included in trend analysis is Silver Bow Creek at Warm Springs, Montana (sampling site 8), and the most downstream site is Clark Fork above Missoula, Montana (sampling site 22), which is just downstream from the former Milltown Dam. Water year is the 12-month period from October 1 through September 30 and is designated by the year in which it ends. Trend analysis was done by using a joint time-series model for concentration and streamflow. To provide temporal resolution of changes in water quality, trend analysis was conducted for four sequential 5-year periods: period 1 (water years 1996–2000), period 2 (water years 2001–5), period 3 (water years 2006–10), and period 4 (water years 2011–15). Because of the substantial effect of the intentional breach of Milltown Dam on March 28, 2008, period 3 was subdivided into period 3A (October 1, 2005–March 27, 2008) and period 3B (March 28, 2008–September 30, 2010) for the Clark Fork above Missoula (sampling site 22). Trend results were considered statistically significant when the statistical probability level was less than 0.01.In conjunction with the trend analysis, estimated normalized constituent loads (hereinafter referred to as “loads”) were calculated and presented within the framework of a constituent-transport analysis to assess the temporal trends in flow-adjusted concentrations (FACs) in the context of sources and transport. The transport analysis allows assessment of temporal changes in relative contributions from upstream source areas to loads transported past each reach outflow.Trend results indicate that FACs of unfiltered-recoverable copper decreased at the sampling sites from the start of period 1 through the end of period 4; the decreases ranged from large for one sampling site (Silver Bow Creek at Warm Springs [sampling site 8]) to moderate for two sampling sites (Clark Fork near Galen, Montana [sampling site 11] and Clark Fork above Missoula [sampling site 22]) to small for four sampling sites (Clark Fork at Deer Lodge, Montana [sampling site 14], Clark Fork at Goldcreek, Montana [sampling site 16], Clark Fork near Drummond, Montana [sampling site 18], and Clark Fork at Turah Bridge near Bonner, Montana [sampling site 20]). For period 4 (water years 2011–15), the most notable changes indicated for the Milltown Reservoir/Clark Fork River Superfund Site were statistically significant decreases in FACs and loads of unfiltered-recoverable copper for sampling sites 8 and 22. The period 4 changes in FACs of unfiltered-recoverable copper for all other sampling sites were not statistically significant.Trend results indicate that FACs of unfiltered-recoverable arsenic decreased at the sampling sites from period 1 through period 4 (water years 1996–2015); the decreases ranged from minor (sampling sites 8–20) to small (sampling site 22). For period 4 (water years 2011–15), the most notable changes indicated for the Milltown Reservoir/Clark Fork River Superfund Site were statistically significant decreases in FACs and loads of unfiltered-recoverable arsenic for sampling site 8 and near statistically significant decreases for sampling site 22. The period 4 changes in FACs of unfiltered-recoverable arsenic for all other sampling sites were not statistically significant.Trend results indicate that FACs of suspended sediment decreased at the sampling sites from period 1 through period 4 (water years 1996–2015); the decreases ranged from moderate (sampling site 8) to small (sampling sites 11–22). For period 4 (water years 2011–15), the changes in FACs of suspended sediment were not statistically significant for any sampling sites.The reach of the Clark Fork from Galen to Deer Lodge is a large source of metallic contaminants and suspended sediment, which strongly affects downstream transport of those constituents. Mobilization of copper and suspended sediment from flood-plain tailings and the streambed of the Clark Fork and its tributaries within the reach results in a contribution of those constituents that is proportionally much larger than the contribution of streamflow from within the reach. Within the reach from Galen to Deer Lodge, unfiltered-recoverable copper loads increased by a factor of about 4 and suspended-sediment loads increased by a factor of about 5, whereas streamflow increased by a factor of slightly less than 2. For period 4 (water years 2011–15), unfiltered-recoverable copper and suspended-sediment loads sourced from within the reach accounted for about 41 and 14 percent, respectively, of the loads at Clark Fork above Missoula (sampling site 22), whereas streamflow sourced from within the reach accounted for about 4 percent of the streamflow at sampling site 22. During water years 1996–2015, decreases in FACs and loads of unfiltered-recoverable copper and suspended sediment for the reach generally were proportionally smaller than for most other reaches.Unfiltered-recoverable copper loads sourced within the reaches of the Clark Fork between Deer Lodge and Turah Bridge near Bonner (just upstream from the former Milltown Dam) were proportionally smaller than contributions of streamflow sourced from within the reaches; these reaches contributed proportionally much less to copper loading in the Clark Fork than the reach between Galen and Deer Lodge. Although substantial decreases in FACs and loads of unfiltered-recoverable copper and suspended sediment were indicated for Silver Bow Creek at Warm Springs (sampling site 8), those substantial decreases were not translated to downstream reaches between Deer Lodge and Turah Bridge near Bonner. The effect of the reach of the Clark Fork from Galen to Deer Lodge as a large source of copper and suspended sediment, in combination with little temporal change in those constituents for the reach, contributes to this pattern.With the removal of the former Milltown Dam in 2008, substantial amounts of contaminated sediments that remained in the Clark Fork channel and flood plain in reach 9 (downstream from Turah Bridge near Bonner) became more available for mobilization and transport than before the dam removal. After the removal of the former Milltown Dam, the Clark Fork above Missoula (sampling site 22) had statistically significant decreases in FACs of unfiltered-recoverable copper in period 3B (March 28, 2008, through water year 2010) that continued in period 4 (water years 2011–15). Also, decreases in FACs of unfiltered-recoverable arsenic and suspended sediment were indicated for period 4 at this site. The decrease in FACs of unfiltered-recoverable copper for sampling site 22 during period 4 was proportionally much larger than the decrease for the Clark Fork at Turah Bridge near Bonner (sampling site 20). Net mobilization of unfiltered-recoverable copper and arsenic from sources within reach 9 are smaller for period 4 than for period 1 when the former Milltown Dam was in place, providing evidence that contaminant source materials have been substantially reduced in reach 9.

Von Economo Neurons and Fork Cells: A Neurochemical Signature Linked to Monoaminergic Function.

PubMed

Dijkstra, Anke A; Lin, Li-Chun; Nana, Alissa L; Gaus, Stephanie E; Seeley, William W

2018-01-01

The human anterior cingulate and frontoinsular cortices are distinguished by 2 unique Layer 5 neuronal morphotypes, the von Economo neurons (VENs) and fork cells, whose biological identity remains mysterious. Insights could impact research on diverse neuropsychiatric diseases to which these cells have been linked. Here, we leveraged the Allen Brain Atlas to evaluate mRNA expression of 176 neurotransmitter-related genes and identified vesicular monoamine transporter 2 (VMAT2), gamma-aminobutyric acid (GABA) receptor subunit θ (GABRQ), and adrenoreceptor α-1A (ADRA1A) expression in human VENs, fork cells, and a minority of neighboring Layer 5 neurons. We confirmed these results using immunohistochemistry or in situ hybridization. VMAT2 and GABRQ expression was absent in mouse cerebral cortex. Although VMAT2 is known to package monoamines into synaptic vesicles, in VENs and fork cells its expression occurs in the absence of monoamine-synthesizing enzymes or reuptake transporters. Thus, VENs and fork cells may possess a novel, uncharacterized mode of cortical monoaminergic function that distinguishes them from most other mammalian Layer 5 neurons. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Numerical performance analysis of quartz tuning fork-based force sensors

NASA Astrophysics Data System (ADS)

Dagdeviren, Omur E.; Schwarz, Udo D.

2017-01-01

Quartz tuning fork-based force sensors where one prong is immobilized onto a holder while the other one is allowed to oscillate freely (‘qPlus’ configuration) are in widespread use for high-resolution scanning probe microscopy applications. Due to the small size of the tuning forks (≈3 mm) and the complexity of the sensor assemblies, the reliable and repeatable manufacturing of the sensors has been challenging. In this paper, we investigate the contribution of the amount and location of the epoxy glue used to attach the tuning fork to its holder on the sensor’s performance. Towards this end, we use finite element analysis to model the entire sensor assembly and to perform static and dynamic numerical simulations. Our analysis reveals that increasing the thickness of the epoxy layer between prong and holder results in a decrease of the sensor’s spring constant, eigenfrequency, and quality factor while showing an increasing deviation from oscillation in its primary modal shape. Adding epoxy at the sides of the tuning fork also leads to a degradation of the quality factor even though in this case, spring constant and eigenfrequency rise in tandem with a lessening of the deviation from its ideal modal shape.

Effects of acid mine drainage on the stream ecosystem of the east fork of the Obey River, Tennessee

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nichols, L.E.; Bulow, F.L.

1973-01-01

The stream ecosystem of the east fork of the Obey River, Tennessee was studied from January through December 1970. Emphasis centered on water quality, macroinvertebrates, fish and aquatic flora affected by acid mine drainage. Two control stations were established within the study area, one located below the zone of pollution. A reservoir station was established to detect any neutralization occurring within Dale Hollow Reservoir below the confluence of the east fork and west fork. An area approximately 40 miles in length was found to be severely degraded by acid mine drainage. Limited macroinvertebrate populations existed within this region. Chironomus andmore » Sialis were the predominate benthic indicator organisms present in the polluted zone. Euglena mutabilis was the most abundant representative organism of the aquatic flora. This organism was found to be characteristic of acid mine pollution. Fish were recovered above and below, but not within, the zone of pollution. Fish recovered were characteristic of the type of habitat in which they were collected, being either typical stream or reservoir fish. A fish kill occurred in the east fork embayment of Dale Hollow on August 15, 1970 from acid mine drainage discharged upstream.« less

The annealing helicase and branch migration activities of Drosophila HARP.

PubMed

Kassavetis, George A; Kadonaga, James T

2014-01-01

HARP (SMARCAL1, MARCAL1) is an annealing helicase that functions in the repair and restart of damaged DNA replication forks through its DNA branch migration and replication fork regression activities. HARP is conserved among metazoans. HARP from invertebrates differs by the absence of one of the two HARP-specific domain repeats found in vertebrates. The annealing helicase and branch migration activity of invertebrate HARP has not been documented. We found that HARP from Drosophila melanogaster retains the annealing helicase activity of human HARP, the ability to disrupt D-loops and to branch migrate Holliday junctions, but fails to regress model DNA replication fork structures. A comparison of human and Drosophila HARP on additional substrates revealed that both HARPs are competent in branch migrating a bidirectional replication bubble composed of either DNA:DNA or RNA:DNA hybrid. Human, but not Drosophila, HARP is also capable of regressing a replication fork structure containing a highly stable poly rG:dC hybrid. Persistent RNA:DNA hybrids in vivo can lead to replication fork arrest and genome instability. The ability of HARP to strand transfer hybrids may signify a hybrid removal function for this enzyme, in vivo.

Accurate aging of juvenile salmonids using fork lengths

USGS Publications Warehouse

Sethi, Suresh; Gerken, Jonathon; Ashline, Joshua

2017-01-01

Juvenile salmon life history strategies, survival, and habitat interactions may vary by age cohort. However, aging individual juvenile fish using scale reading is time consuming and can be error prone. Fork length data are routinely measured while sampling juvenile salmonids. We explore the performance of aging juvenile fish based solely on fork length data, using finite Gaussian mixture models to describe multimodal size distributions and estimate optimal age-discriminating length thresholds. Fork length-based ages are compared against a validation set of juvenile coho salmon, Oncorynchus kisutch, aged by scales. Results for juvenile coho salmon indicate greater than 95% accuracy can be achieved by aging fish using length thresholds estimated from mixture models. Highest accuracy is achieved when aged fish are compared to length thresholds generated from samples from the same drainage, time of year, and habitat type (lentic versus lotic), although relatively high aging accuracy can still be achieved when thresholds are extrapolated to fish from populations in different years or drainages. Fork length-based aging thresholds are applicable for taxa for which multiple age cohorts coexist sympatrically. Where applicable, the method of aging individual fish is relatively quick to implement and can avoid ager interpretation bias common in scale-based aging.

Preliminary assessment of aggradation potential in the North Fork Stillaguamish River downstream of the State Route 530 landslide near Oso, Washington

USGS Publications Warehouse

Magirl, Christopher S.; Keith, Mackenzie K.; Anderson, Scott W.; O'Connor, Jim; Robert Aldrich,; Mastin, Mark C.

2015-12-28

On March 22, 2014, the State Route 530 Landslide near Oso, Washington, traveled almost 2 kilometers (km), destroyed more than 40 structures, and impounded the North Fork Stillaguamish River to a depth of 8 meters (m) and volume of 3.3×106 cubic meters (m3). The landslide killed 43 people. After overtopping and establishing a new channel through the landslide, the river incised into the landslide deposit over the course of 10 weeks draining the impoundment lake and mobilizing an estimated 280,000±56,000 m3 of predominantly sand-sized and finer sediment. During the first 4 weeks after the landslide, this eroded sediment caused downstream riverbed aggradation of 1–2 m within 1 km of the landslide and 0.4 m aggradation at Whitman Road Bridge, 3.5 km downstream. Winter high flows in 2014–15 were anticipated to mobilize an additional 220,000±44,000 m3 of sediment, potentially causing additional aggradation and exacerbating flood risk downstream of the landslide. Analysis of unit stream power and bed-material transport capacity along 35 km of the river corridor indicated that most fine-grained sediment will transport out of the North Fork Stillaguamish River, although some localized additional aggradation was possible. This new aggradation was not likely to exceed 0.1 m except in reaches within a few kilometers downstream of the landslide, where additional aggradation of up to 0.5 m is possible. Alternative river response scenarios, including continued mass wasting from the landslide scarp, major channel migration or avulsion, or the formation of large downstream wood jams, although unlikely, could result in reaches of significant local aggradation or channel change.

Remedial investigation work plan for the Upper East Fork Poplar Creek characterization area, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-09-01

The Oak Ridge Y-12 Plant, located within the Oak Ridge Reservation (ORR), is owned by the US Department of Energy (DOE) and managed by Lockheed Martin Energy Systems, Inc. The entire ORR was placed on the National Priorities List (NPL) of CERCLA sites in November 1989. Following CERCLA guidelines, sites under investigation require a remedial investigation (RI) to define the nature and extent of contamination, evaluate the risks to public health and the environment, and determine the goals for a feasibility study (FS) of potential remedial actions. The need to complete RIs in a timely manner resulted in the establishmentmore » of the Upper East Fork Poplar Creek (UEFPC) Characterization Area (CA) and the Bear Creek CA. The CA approach considers the entire watershed and examines all appropriate media within it. The UEFPC CA, which includes the main Y-12 Plant area, is an operationally and hydrogeologically complex area that contains numerous contaminants and containment sources, as well as ongoing industrial and defense-related activities. The UEFPC CA also is the suspected point of origin for off-site groundwater and surface-water contamination. The UEFPC CA RI also will address a carbon-tetrachloride/chloroform-dominated groundwater plume that extends east of the DOE property line into Union Valley, which appears to be connected with springs in the valley. In addition, surface water in UEFPC to the Lower East Fork Poplar Creek CA boundary will be addressed. Through investigation of the entire watershed as one ``site,`` data gaps and contaminated areas will be identified and prioritized more efficiently than through separate investigations of many discrete units.« less

Valley aggradation in the San Gabriel Mountains, California: climate change versus catastrophic landslide

NASA Astrophysics Data System (ADS)

Scherler, D.; Lamb, M. P.; Rhodes, E. J.; Avouac, J. P.

2014-12-01

The San Gabriel Mountains (SGM) in Southern California, rate amongst the most rapidly uplifting and eroding mountains in the United States. Their steep slopes and sensitivity to wildfires, flash floods, landslides, and debris flows account for imminent hazards to nearby urban areas that might be accentuated by climatic and other environmental changes. Previous studies suggested that river terraces along the North Fork of the San Gabriel River, record temporal variations in sediment supply and river transport capacity that are representative for the SGM and related to climatic changes during the Quaternary. Based on field observations, digital topographic analysis, and dating of Quaternary deposits, we suggest that valley aggradation in the North Fork San Gabriel Canyon was spatially confined and a consequence of the sudden supply of unconsolidated material to upstream reaches by one of the largest known landslides in the SGM. New 10Be-derived surface exposure ages from the landslide deposits, previously assumed to be early to middle Pleistocene in age, indicate at least three Holocene events at ~8-9 ka, ~4-5 ka, and ~0.5-1 ka. The oldest landslide predates the valley aggradation period, which is constrained by existing 14C ages and new luminescence ages to ~7-8 ka. The spatial distribution, morphology, and sedimentology of the river terraces are consistent with deposition from far-travelling debris flows that originated within the landslide deposits. Valley aggradation in the North Fork San Gabriel Canyon therefore resulted from locally enhanced sediment supply that temporarily overwhelmed river capacity but the lack of similar deposits in other parts of the SGM argues against a regional climatic signal. So far, there exists no evidence that in the San Gabriel Mountains, climatic changes can cause sustained increases in hillslope sediment supply that lead to river aggradation and terrace formation.

Evaluating turbidity and suspended-sediment concentration relations from the North Fork Toutle River basin near Mount St. Helens, Washington; annual, seasonal, event, and particle size variations - a preliminary analysis.

USGS Publications Warehouse

Uhrich, Mark A.; Spicer, Kurt R.; Mosbrucker, Adam; Christianson, Tami

2015-01-01

Regression of in-stream turbidity with concurrent sample-based suspended-sediment concentration (SSC) has become an accepted method for producing unit-value time series of inferred SSC (Rasmussen et al., 2009). Turbidity-SSC regression models are increasingly used to generate suspended-sediment records for Pacific Northwest rivers (e.g., Curran et al., 2014; Schenk and Bragg, 2014; Uhrich and Bragg, 2003). Recent work developing turbidity-SSC models for the North Fork Toutle River in Southwest Washington (Uhrich et al., 2014), as well as other studies (Landers and Sturm, 2013, Merten et al., 2014), suggests that models derived from annual or greater datasets may not adequately reflect shorter term changes in turbidity-SSC relations, warranting closer inspection of such relations. In-stream turbidity measurements and suspended-sediment samples have been collected from the North Fork Toutle River since 2010. The study site, U.S. Geological Survey (USGS) streamgage 14240525 near Kid Valley, Washington, is 13 river km downstream of the debris avalanche emplaced by the 1980 eruption of Mount St. Helens (Lipman and Mullineaux, 1981), and 2 river km downstream of the large sediment retention structure (SRS) built from 1987–1989 to mitigate the associated sediment hazard. The debris avalanche extends roughly 25 km down valley from the edifice of the volcano and is the primary source of suspended sediment moving past the streamgage (NF Toutle-SRS). Other significant sources are debris flow events and sand deposits upstream of the SRS, which are periodically remobilized and transported downstream. Also, finer material often is derived from the clay-rich original debris avalanche deposit, while coarser material can derive from areas such as fluvially reworked terraces.

CANVAS FINISH TO REMOVE FLOAT MARKS. View is to the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

CANVAS FINISH TO REMOVE FLOAT MARKS. View is to the northwest of deck finishing operations - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

27 CFR 9.65 - North Fork of Roanoke.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the North... and 697 in Roanoke County. (2) Then the boundary follows State Route 697 northeast over Crawford Ridge...

27 CFR 9.65 - North Fork of Roanoke.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the North... and 697 in Roanoke County. (2) Then the boundary follows State Route 697 northeast over Crawford Ridge...

14. View to southwest. View through truss along centerline from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. View to southwest. View through truss along centerline from below deck. (65mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

20. View to southeast. Aerial view of bridge in setting; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. View to southeast. Aerial view of bridge in setting; downstream side. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

COUNTERWEIGHT FOOTINGS ON EAST SIDE. View is to the north ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

COUNTERWEIGHT FOOTINGS ON EAST SIDE. View is to the north from the old suspension bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

13. View to northeast. View along centerline from Humboldt County ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. View to northeast. View along centerline from Humboldt County side of bridge. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

JIGGER STICK USED IN ERECTION. Shown on highline, direction of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

JIGGER STICK USED IN ERECTION. Shown on highline, direction of view is probably to the north - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

12. View to southwest. View along centerline from Trinity County ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. View to southwest. View along centerline from Trinity County side of bridge. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

11. View to southeast. More distant overview of bridge in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. View to southeast. More distant overview of bridge in setting; downstream side. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

26. MOORSE DRILL CABINET AND FORK ART FABRICATED AT SHOP ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. MOORSE DRILL CABINET AND FORK ART FABRICATED AT SHOP (L TO R)- LOOKING SOUTHEAST. - W. A. Young & Sons Foundry & Machine Shop, On Water Street along Monongahela River, Rices Landing, Greene County, PA

Concentrations and loads of cadmium, lead, and zinc measured near the peak of the 1999 snowmelt-runoff hydrographs for 42 water-quality stations, Coeur d'Alene River basin, Idaho

USGS Publications Warehouse

Woods, Paul F.

2000-01-01

The Coeur d’Alene River near Harrison transported 924 pounds of dissolved lead per day, of which 82.8 pounds came from the South Fork and 11.7 pounds from the North Fork. Only 10.2 percent of the load at Harrison was measured at the Pinehurst and Enaville stations; therefore, a substantial load of dissolved lead is being contributed downstream from the confluence of the North and South Forks.

Cultural Resources Reconnaissance for Proposed Flood Control Measures at Cramerton, Gaston County, North Carolina.

DTIC Science & Technology

1982-01-28

by the South Fork of the 6 I. .. . ... . .. . . . Catawba Piver. The South Fork is a major upper tributary of the Santee drainage system. :t rises in...streams in the survey area are first order tributaries . Several of the smaller streams are not depicted an the 1:24,000 topographic sheet. The South Fork...of i-ost.oid O’. Cultura aate’rial density in the s=r2eJ and deflated sample area is 0.35 piecei1.0 szuare meter. Anite quartz ncdules w€ere eroded

The Sound Field Around a Tuning Fork and the Role of a Resonance Box

NASA Astrophysics Data System (ADS)

Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

2015-02-01

Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: —Why is a bare tuning fork such a weak emitter of sound?—What is the role of the resonance box?—Where does energy connected with larger intensity of emitted acoustic waves come from?

Water-Quality, Bed-Sediment, and Biological Data (October 2006 through September 2007) and Statistical Summaries of Long-Term Data for Streams in the Clark Fork Basin, Montana

USGS Publications Warehouse

Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

2008-01-01

Water, bed sediment, and biota were sampled in streams from Butte to below Milltown Reservoir as part of a long-term monitoring program in the upper Clark Fork basin; additional water-quality samples were collected in the Clark Fork basin from sites near Milltown Reservoir downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water-quality samples were collected periodically at 22 sites from October 2006 through September 2007. Bed-sediment and biological samples were collected once at 12 sites during August 2007. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2006 through September 2007. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Turbidity was analyzed for samples collected at sites where seasonal daily values of turbidity were being determined. Nutrients also were analyzed in the supplemental water-quality samples. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for four sites, and seasonal daily values of turbidity were determined for five sites. Bed-sediment data include trace-element concentrations in the fine-grained fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

37. BRIDGE 115, SMITH RIVER MIDDLE FORK OREGON STATE HIGHWAY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

37. BRIDGE 1-15, SMITH RIVER MIDDLE FORK OREGON STATE HIGHWAY 199. JOSEPHINE COUNTY, OREGON. LOOKING SSW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

2. GENERAL VIEW OF BRIDGE FROM ROADBED WITH 4' RANGE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. GENERAL VIEW OF BRIDGE FROM ROADBED WITH 4' RANGE POLE NEAR NORTHWEST CORNER OF BRIDGE, LOOKING SOUTH - North Fork Bridge, Spans North Fork of White River at State Highway 5, Norfork, Baxter County, AR

PIER 2. View is to the northeast, looking from Pier ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PIER 2. View is to the northeast, looking from Pier 1 toward Pier 2 from beneath completed bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

3. View to southwest. Oblique view of downstream side of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. View to southwest. Oblique view of downstream side of bridge and west pier. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

2. View to east. Oblique view of downstream side of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. View to east. Oblique view of downstream side of bridge and east pier. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

9. View to northeast. Oblique view of upstream side of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. View to northeast. Oblique view of upstream side of bridge from approximately deck level. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

PLACING TOP CHORD CENTER PANEL. View to the northwest from ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PLACING TOP CHORD CENTER PANEL. View to the northwest from the old suspension bridge. Chord members in place - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

17. View to west. Detail, connection point L2 (see plans), ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. View to west. Detail, connection point L2 (see plans), from below deck. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

35. CHARGING DOOR OF CUPOLA FORM LOFT, WITH FORKS FOR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. CHARGING DOOR OF CUPOLA FORM LOFT, WITH FORKS FOR FEEDING COKE, FOUNDRY BELOW-LOOKING NORTH. - W. A. Young & Sons Foundry & Machine Shop, On Water Street along Monongahela River, Rices Landing, Greene County, PA

Tuning Forks and Monitor Screens.

ERIC Educational Resources Information Center

Harrison, M. A. T.

2000-01-01

Defines the vibrations of a tuning fork against a computer monitor screen as a pattern that can illustrate or explain physical concepts like wave vibrations, wave forms, and phase differences. Presents background information and demonstrates the experiment. (Author/YDS)

6. VIEW FACING EAST ALONG NORTH FACE OF BRIDGE AT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. VIEW FACING EAST ALONG NORTH FACE OF BRIDGE AT CONSTRUCTION DETAILS OF WOOD RAILINGS AND STONE ABUTMENTS. - South Fork Tuolumne River Bridge, Spanning South Fork Tuolumne River on Tioga Road, Mather, Tuolumne County, CA

17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK THAT MIXED COFFEE BEANS AS THEY WERE HUSKED - Hacienda Cafetalera Santa Clara, Coffee Mill, KM 19, PR Route 372, Hacienda La Juanita, Yauco Municipio, PR

Chemical bond imaging using higher eigenmodes of tuning fork sensors in atomic force microscopy

NASA Astrophysics Data System (ADS)

Ebeling, Daniel; Zhong, Qigang; Ahles, Sebastian; Chi, Lifeng; Wegner, Hermann A.; Schirmeisen, André

2017-05-01

We demonstrate the ability of resolving the chemical structure of single organic molecules using non-contact atomic force microscopy with higher normal eigenmodes of quartz tuning fork sensors. In order to achieve submolecular resolution, CO-functionalized tips at low temperatures are used. The tuning fork sensors are operated in ultrahigh vacuum in the frequency modulation mode by exciting either their first or second eigenmode. Despite the high effective spring constant of the second eigenmode (on the order of several tens of kN/m), the force sensitivity is sufficiently high to achieve atomic resolution above the organic molecules. This is observed for two different tuning fork sensors with different tip geometries (small tip vs. large tip). These results represent an important step towards resolving the chemical structure of single molecules with multifrequency atomic force microscopy techniques where two or more eigenmodes are driven simultaneously.

General Reevaluation and Supplement to Environmental Impact Statement for Flood Control and Related Purposes. Red and Red Lake Rivers at East Grand Forks, Minnesota.

DTIC Science & Technology

1984-11-01

ORGANIZATION (if applicable) 8c. ADDRESS (City, State, and ZIP Code) 10. SOURCE OF FUNDING NUMBERS PROGRAM PROJECT TASK IWORK UNIT ELEMENT NO. NO. NO...participate in tne project. The city has also entered the regular phase of tne National Flood Insurance program adopted 23 September 1977. The State ’V of...releases It o Possible sites outside area of city control/ during periods of low flow. responsibility. -s Red Lake Watersned District has a current program

Is there sufficient evidence for tuning fork tests in diagnosing fractures? A systematic review.

PubMed

Mugunthan, Kayalvili; Doust, Jenny; Kurz, Bodo; Glasziou, Paul

2014-08-04

To determine the diagnostic accuracy of tuning fork tests for detecting fractures. Systematic review of primary studies evaluating the diagnostic accuracy of tuning fork tests for the presence of fracture. We searched MEDLINE, CINAHL, AMED, EMBASE, Sports Discus, CAB Abstracts and Web of Science from commencement to November 2012. We manually searched the reference lists of any review papers and any identified relevant studies. Two reviewers independently reviewed the list of potentially eligible studies and rated the studies for quality using the QUADAS-2 tool. Data were extracted to form 2×2 contingency tables. The primary outcome measure was the accuracy of the test as measured by its sensitivity and specificity with 95% CIs. We included six studies (329 patients), with two types of tuning fork tests (pain induction and loss of sound transmission). The studies included patients with an age range 7-60 years. The prevalence of fracture ranged from 10% to 80%. The sensitivity of the tuning fork tests was high, ranging from 75% to 100%. The specificity of the tests was highly heterogeneous, ranging from 18% to 95%. Based on the studies in this review, tuning fork tests have some value in ruling out fractures, but are not sufficiently reliable or accurate for widespread clinical use. The small sample size of the studies and the observed heterogeneity make generalisable conclusion difficult. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

15. View to northeast. View along centerline from below deck; ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. View to northeast. View along centerline from below deck; detail of bent and floor beams. (65mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

STEEL ERECTION. View of upstream side of bridge, looking north ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

STEEL ERECTION. View of upstream side of bridge, looking north from the old suspension bridge at unjoined cantilever arms - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

16. View to southsouthwest. Detail, connection point U1 (see plans), ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. View to south-southwest. Detail, connection point U1 (see plans), from below deck. (135mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

LOWER CHORD ERECTION. View is to northnorthwest from the old ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOWER CHORD ERECTION. View is to north-northwest from the old suspension bridge. Chord members suspended from jigger stick - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

STEEL ERECTION. View is to the north from the old ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

STEEL ERECTION. View is to the north from the old suspension bridge, looking at upstream side of new bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

4. View to westsouthwest. Oblique view of upstream side of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. View to west-southwest. Oblique view of upstream side of bridge from approximately deck level. (90mm lens) - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

117. Laurel Fork Viaduct. Elevation view of this 545 1939 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

117. Laurel Fork Viaduct. Elevation view of this 545 1939 steel girder viaduct. Example of structure with plain reinforced concrete arches. Looking northwest. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

78 FR 48901 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-12

..., 13000637 NORTH DAKOTA Grand Forks County Hariman Sanatorium, 2002 University Ave., Grand Forks, 13000633... Municipal de la Playa de Ponce, 28 Alfonso XII St., Ponce, 13000639 UTAH Salt Lake County Bennion, Howard...

Sequence-stratigraphic controls on sandstone diagenesis: An example from the Williams Fork formation, Piceance Basin, Colorado

NASA Astrophysics Data System (ADS)

Aboktef, Adel

This study documents the distribution of diagenetic alterations in Williams Fork fluvial sandstones, assess sequence stratigraphic controls on diagenetic features, and addresses diagenetic impacts on porosity. Petrographic point counts of 220 thin sections from six wells forms the database. The near absence of potassium feldspar and volcanic rock fragments in the lower Williams Fork interval and increasing plagioclase content upward represent changes in sediment provenance rather than stratigraphic variability in diagenesis. The lower Williams Fork sands are from sedimentary sources whereas middle and upper Williams Fork sands include input from magmatic arcs and basement uplifts. Compaction, early and late cementation, dissolution, and replacement by calcite or clay minerals combined to alter Williams Fork sandstones. Infiltration of clays occurred prior to any burial. Chlorite, quartz, non-ferroan calcite, compaction and dissolution features, and kaolinite formed during eo-diagenesis at <70°C. More quartz, compaction and dissolution features, plus albite, illite, mixed-layer illite/smectite, ferroan calcite, and dolomite formed in the meso-diagenetic realm (>70°C). Four of these features show spatial variability with respect to systems tracts. Infiltrated clays are concentrated in lowstand systems tracts (LST) and highstand systems tracts (HST) because accommodation space rose slow or fell during deposition of those sands, which led to prolonged sand body exposure on floodplain and ample opportunities for downward percolation of mud during flood events. Concentration of pseudomatrix (mud intraclasts) in HST and LST deposits resulted from floodplain erosion when base-level fell with decreasing accommodation space. Authigenic chlorite formed in the HST and transgressive systems tracts (TST) of the upper half of the Williams Fork Formation because volcanic clasts are abundant in that interval. Quartz overgrowths are more likely to exceed 7% in TST deposits for reasons that are unknown. High total clay content (infiltrated, grain coatings, pseudomatrix) does inhibit quartz overgrowths in all systems tracts. Williams Fork sandstones form low-permeability tight-gas reservoirs. Primary porosity was almost entirely destroyed by compaction and cementation. Reservoir rock resulted from one of two pathways. Eogenetic authigenic chlorite and/or calcite inhibited quartz cementation, minimized compaction and protected some primary porosity. Alternately, dissolution of framework grains or cements created secondary porosity. The later pathway tends to be the more dominant.

Water Quality and Biological Characteristics of the Middle Fork of the Saline River, Arkansas, 2003-06

USGS Publications Warehouse

Galloway, Joel M.; Petersen, James C.; Shelby, Erica L.; Wise, Jim A.

2008-01-01

The Middle Fork of the Saline River has many qualities that have been recognized by State and Federal agencies. The Middle Fork provides habitat for several rare aquatic species and is part of a larger stream system (the Upper Saline River) that is known for relatively high levels of species richness and relatively high numbers of species of concern. Water-quality samples were collected and streamflow was measured by the U.S. Geological Survey at three sites in the Middle Fork Basin between October 2003 and October 2006. The Arkansas Department of Environmental Quality collected discrete synoptic water-quality samples from eight sites between January 2004 and October 2006. The Arkansas Department of Environmental Quality also sampled fish (September-October 2003) and benthic macroinvertebrate communities (September 2003-December 2005) at five sites. Streamflow varied annually among the three streamflow sites from October 2003 to October 2006. The mean annual streamflow for Brushy Creek near Jessieville (MFS06) was 0.72 cubic meters per second for water years 2004-2006. The Middle Fork below Jessieville (MFS05) had a mean annual streamflow of 1.11 cubic meters per second for water years 2004-2006. The Middle Fork near Owensville (MFS02), the most downstream site, had a mean annual streamflow of 3.01 cubic meters per second. The greatest streamflows at the three sites generally occurred in the winter and spring and the least in the summer. Nutrient dynamics in the Middle Fork are controlled by activities in the basin and processes that occur in the stream. Point sources and nonpoint sources of nutrients occur in the Middle Fork Basin that could affect the water-quality. Nitrogen and phosphorus concentrations generally were greatest in Mill Creek (MFS04E) and in the Middle Fork immediately downstream from the confluence with Mill Creek (MFS04) with decreasing concentrations at sites farther downstream in Middle Fork. The site in Mill Creek is located downstream from a wastewater-treatment plant discharge and concentrations at sites farther downstream probably had lesser concentrations because of dilution effects and from algal uptake. Nutrient concentrations generally were significantly greater during high-flow conditions compared to base-flow conditions. Flow-weighted nutrient concentrations were computed for the three streamflow sites and were compared to 82 relatively undeveloped sites identified across the Nation, to the Alum Fork of the Saline River near Reform, Arkansas, and to the Illinois River south of Siloam Springs, Arkansas, a site influenced by numerous point and nonpoint sources of nutrients. Annual flow-weighted nutrient concentrations for MFS06, MFS05, and MFS02 were greater than relatively undeveloped sites, but were substantially less than the Illinois River south of Siloam Springs. Fecal indicator bacteria concentrations were slightly greater at MFS06 and MFS05 compared to concentrations at MFS02 for October 2003 to October 2006. MFS05 had the greatest E.coli concentrations and MFS06 had the greatest fecal coliform concentrations. Overall, fecal indicator bacteria concentrations were significantly greater for samples collected during high-flow conditions compared to samples collected during low-flow conditions at all three sites. Suspended-sediment concentrations did not vary significantly among MFS06, MFS05, and MFS02 for all the samples collected from October 2003 to October 2006. Suspended-sediment concentrations were significantly greater in samples collected during high-flow conditions compared to samples collected during base-flow conditions. Synoptic samples indicated varied total suspended-solids distributions from upstream to downstream in the Middle Fork between January 2004 and October 2006. Overall, total suspended-solids values were the greatest at site MFS02 and decreased at sites upstream and downstream. Turbidity measured when water-quality samples were

PLACING DIAGONALS IN CENTER PANEL. View is northnorthwest from the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PLACING DIAGONALS IN CENTER PANEL. View is north-northwest from the old suspension bridge, looking at upstream side of new bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

PLANT AND PIER #2 EXCAVATION. View is to the northeast, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PLANT AND PIER #2 EXCAVATION. View is to the northeast, looking from Humboldt County side of river toward Trinity County side - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

MOVING SHAFT FORMS FROM PIER #2 TO PIER #1. View ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

MOVING SHAFT FORMS FROM PIER #2 TO PIER #1. View is to the northeast, with shaft forms being moved by highline - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

75 FR 51104 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-18

.... Blome Granitoid Pavement in Grand Forks Boundary Increase, Lewis Blvd between Conklin and Fenton Ave, Lewis Blvd between Fenton Ave and Seward Ave; Woodland Ave between S 4th and Grand Forks, 10000605...

8. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading crane, manufactured by Cleveland Tramrail, 2-1/2 ton capacity. - Juniata Shops, Erecting Shop & Machine Shop, East of Fourth Avenue, between Fourth & Fifth Streets, Altoona, Blair County, PA

9. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading crane, manufactured by Cleveland Tramrail, 2-1/2 ton capacity. - Juniata Shops, Erecting Shop & Machine Shop, East of Fourth Avenue, between Fourth & Fifth Streets, Altoona, Blair County, PA

Detail view of the concrete arched balustrade railing and bushhammered ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail view of the concrete arched balustrade railing and bush-hammered inset posts - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

Drainage Areas of Streams at Selected Locations in Kentucky

DTIC Science & Technology

1981-01-01

23󈧸", Long 82 ° 17󈧇" 195 LICK CREEK .2 .3 .70 1 .81 03207995 Levisa Fork (Fishtrap Lake ) near Millard--Lat 37°26󈧄", Long 82 ° 24󈧾" 195 MILLARD...130 .1 209 .3 392 1,015 (Area in Kentucky) 84 .6 219 (Area outside Kentucky) 308 798 03208000 Levisa Fork (below Fishtrap Lake ) near Millard--Lat 37...547 1,417 Elkhorn Creek Basin--at Russell Fork mile 12 .3 Little Elkhorn Creek at Jenkins--Lat 37 ° 10󈧊", Long 82°38󈧊", at Elkhorn Lake outlet

Grand Forks - East Grand Forks Urban Water Resources Study. Grand Forks Flood Fight Manual

DTIC Science & Technology

1981-07-01

insurance is available for residential and commercial structures and contents at actuarial rates. As of 30 April 1980, 1258 policies were in effect...STATION #3 (WW) CLOSE SHEAR OATE ON LEW« M.VD. ON RIVER SOE OF IMKE. (S) PUCE PUMP M CATCH ■ ASM ON OTHER «DC OF MKE CLOSE SHEAR OATE ON OKE AT ISIS mVBMM...DRIVE. (S>* PUCE PUMP M CATCH « ASM ON OTHER SBC OF (NKE CLOU SHEAR OATE AT UFT STATION «S M MANHOU «WW> SWITCH OVER UFT STATION «I - CLOSE OATE

Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante

Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteinsmore » retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. We suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal.« less

Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling.

PubMed

Cobb, Andrew M; Murray, Thomas V; Warren, Derek T; Liu, Yiwen; Shanahan, Catherine M

2016-09-02

The accumulation of prelamin A is linked to disruption of cellular homeostasis, tissue degeneration and aging. Its expression is implicated in compromised genome stability and increased levels of DNA damage, but to date there is no complete explanation for how prelamin A exerts its toxic effects. As the nuclear lamina is important for DNA replication we wanted to investigate the relationship between prelamin A expression and DNA replication fork stability. In this study we report that the expression of prelamin A in U2OS cells induced both mono-ubiquitination of proliferating cell nuclear antigen (PCNA) and subsequent induction of Pol η, two hallmarks of DNA replication fork stalling. Immunofluorescence microscopy revealed that cells expressing prelamin A presented with high levels of colocalisation between PCNA and γH2AX, indicating collapse of stalled DNA replication forks into DNA double-strand breaks. Subsequent protein-protein interaction assays showed prelamin A interacted with PCNA and that its presence mitigated interactions between PCNA and the mature nuclear lamina. Thus, we propose that the cytotoxicity of prelamin A arises in part, from it actively competing against mature lamin A to bind PCNA and that this destabilises DNA replication to induce fork stalling which in turn contributes to genomic instability.

Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

DOE PAGES

Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante; ...

2015-09-08

Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteinsmore » retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. We suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal.« less

Antibiotic Resistance Patterns of Major Zoonotic Pathogens from All-Natural, Antibiotic-Free, Pasture-Raised Broiler Flocks in the Southeastern United States.

PubMed

Rothrock, Michael J; Hiett, Kelli L; Guard, Jean Y; Jackson, Charlene R

2016-03-01

The use of antibiotics in agroecosystems has been implicated in the rise in antibiotic resistance (AR), which can affect environmental, animal, and human health. To determine the environmental impact of antibiotic use in agroecosystems, appropriate background levels of AR in agricultural environments in the absence of antibiotic application must be determined. Therefore, to determine background levels of AR in broiler production, four target microbes (, , , and ) were isolated from 15 all-natural, antibiotic-free, pasture-raised broiler flocks from six farms within the southeastern United States. The AR profiles of these isolates were characterized using the CDC National Antimicrobial Resistance Monitoring System for Enteric Bacteria (NARMS), and these resistance patterns were compared across target microbes and farms and throughout the life cycle of the flocks along the farm-to-fork continuum. Antibiotic resistances were most prevalent in and and least prevalent in . Although and were isolated from the same farms and characterized using the same NARMS plates, they exhibited distinct AR profiles, with demonstrating clear farm-specific resistance patterns. Multidrug resistance rates (three or more antibiotics), in order of prevalence, were (63.9%), (36.0%), (12.7%), and (1.4%). The results of this study demonstrate the variability in background AR among major food safety-related microbes, even when isolated from similar production and processing samples from the same farms, and indicate the need for the proper design of future broiler production studies to account for this highly dynamic background AR. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

Water-quality trends for selected sampling sites in the upper Clark Fork Basin, Montana, water years 1996-2010

USGS Publications Warehouse

Sando, Steven K.; Vecchia, Aldo V.; Lorenz, David L.; Barnhart, Elliott P.

2014-01-01

A large-scale trend analysis was done on specific conductance, selected trace elements (arsenic, cadmium, copper, iron, lead, manganese, and zinc), and suspended-sediment data for 22 sites in the upper Clark Fork Basin for water years 1996–2010. Trend analysis was conducted by using two parametric methods: a time-series model (TSM) and multiple linear regression on time, streamflow, and season (MLR). Trend results for 1996–2010 indicate moderate to large decreases in flow-adjusted concentrations (FACs) and loads of copper (and other metallic elements) and suspended sediment in Silver Bow Creek upstream from Warm Springs. Deposition of metallic elements and suspended sediment within Warm Springs Ponds substantially reduces the downstream transport of those constituents. However, mobilization of copper and suspended sediment from floodplain tailings and stream banks in the Clark Fork reach from Galen to Deer Lodge is a large source of metallic elements and suspended sediment, which also affects downstream transport of those constituents. Copper and suspended-sediment loads mobilized from within this reach accounted for about 40 and 20 percent, respectively, of the loads for Clark Fork at Turah Bridge (site 20); whereas, streamflow contributed from within this reach only accounted for about 8 percent of the streamflow at Turah Bridge. Minor changes in FACs and loads of copper and suspended sediment are indicated for this reach during 1996–2010. Clark Fork reaches downstream from Deer Lodge are relatively smaller sources of metallic elements than the reach from Galen to Deer Lodge. In general, small decreases in loads and FACs of copper and suspended sediment are indicated for Clark Fork sites downstream from Deer Lodge during 1996–2010. Thus, although large decreases in FACs and loads of copper and suspended sediment are indicated for Silver Bow Creek upstream from Warm Springs, those large decreases are not translated to the more downstream reaches largely because of temporal stationarity in constituent transport relations in the Clark Fork reach from Galen to Deer Lodge. Unlike metallic elements, arsenic (a metalloid element) in streams in the upper Clark Fork Basin typically is mostly in dissolved phase, has less variability in concentrations, and has weaker direct relations with suspended-sediment concentrations and streamflow. Arsenic trend results for 1996–2010 indicate generally moderate decreases in FACs and loads in Silver Bow Creek upstream from Opportunity. In general, small temporal changes in loads and FACs of arsenic are indicated for Silver Bow Creek and Clark Fork reaches downstream from Opportunity during 1996–2010. Contribution of arsenic (from Warm Springs Ponds, the Mill-Willow bypass, and groundwater sources) in the Silver Bow Creek reach from Opportunity to Warm Springs is a relatively large source of arsenic. Arsenic loads originating from within this reach accounted for about 11 percent of the load for Clark Fork at Turah Bridge; whereas, streamflow contributed from within this reach only accounted for about 2 percent of the streamflow at Turah Bridge.

12. Sewage Ejector Pumps, view to the southwest. These pumps ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Sewage Ejector Pumps, view to the southwest. These pumps are connected to sewage treatment tanks. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

POURING FOOTING OF PIER #1. View is to the southwest, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

POURING FOOTING OF PIER #1. View is to the southwest, looking from Pier 2 in Trinity County toward Pier 1 in Humboldt County - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

COUNTERWEIGHT ON WEST SIDE. View is to the northwest of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

COUNTERWEIGHT ON WEST SIDE. View is to the northwest of counterweight on Humboldt County end of bridge, probably taken from the old suspension bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Detail view of substructure, view looking south at the center ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail view of substructure, view looking south at the center in-water frame bent - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

77 FR 66541 - Safety Zone; Alliance Road Bridge Demolition; Black Warrior River, Locust Fork; Birmingham, AL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-06

... 1625-AA00 Safety Zone; Alliance Road Bridge Demolition; Black Warrior River, Locust Fork; Birmingham... of the Alliance Road Bridge (Co. Rd. 61). Entry into, transiting or anchoring in this zone is.... [[Page 66542

Remedial Investigation Work Plan for Upper East Fork Poplar Creek Operable Unit 3 at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. Environmental Restoration Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1993-08-01

Upper East Fork Popular Creek Operable Unit 3 (UEFPC OU 3) is a source term OU composed of seven sites, and is located in the western portion of the Y-12 Plant. For the most part, the UEFPC OU 3 sites served unrelated purposes and are geographically removed from one another. The seven sites include the following: Building 81-10, the S-2 Site, Salvage Yard oil storage tanks, the Salvage Yard oil/solvent drum storage area, Tank Site 2063-U, the Salvage Yard drum deheader, and the Salvage Yard scrap metal storage area. All of these sites are contaminated with at least one ormore » more hazardous and/or radioactive chemicals. All sites have had some previous investigation under the Y-12 Plant RCRA Program. The work plan contains summaries of geographical, historical, operational, geological, and hydrological information specific to each OU 3 site. The potential for release of contaminants to receptors through various media is addressed, and a sampling and analysis plan is presented to obtain objectives for the remedial investigation. Proposed sampling activities are contingent upon the screening level risk assessment, which includes shallow soil sampling, soil borings, monitoring well installation, groundwater sampling, and surface water sampling. Data from the site characterization activities will be used to meet the above objectives. A Field Sampling Investigation Plan, Health and Safety Plan, and Waste Management Plan are also included in this work plan.« less

Patterns of genetic differentiation and conservation of the slabside pearlymussel, Lexingtonia dolabelloides (Lea, 1840) in the Tennessee River drainage

USGS Publications Warehouse

Grobler, P.J.; Jones, J.W.; Johnson, N.A.; Beaty, B.; Struthers, J.; Neves, R.J.; Hallerman, E.M.

2006-01-01

The restoration and recovery of imperiled mussel species will require the re-establishment of populations into historically occupied habitats. The possible existence of genetic differentiation among populations should be considered before inter-basin transfers are made. Eighty individuals of the federal candidate species Lexingtonia dolabelloides were sampled from populations in the North Fork Holston, Middle Fork Holston, Clinch, Paint Rock and Duck rivers of the Tennessee River basin in the southeastern United States. We sequenced 603 base-pairs of a mitochondrial DNA gene (ND-1) and 512 base-pairs of a nuclear DNA gene (ITS-1). Analyses of molecular variation (AMOVA) values for both genes indicated that the majority of variation in L. dolabelloides resided within populations (82.9-88.3%), with 11.7-17.1% of variation among populations. Haplotype frequencies differed significantly among populations for both genes sequenced. Clustering of haplotypes in minimum-spanning networks did not conform stringently to population boundaries, reflecting high within-population and low between-population variability. Maximum parsimony analysis did not identify any population as a monophyletic lineage. A Mantel test showed no significant correlation between geographical stream distance and genetic distance, thus not supporting a pattern of isolation-by-distance. Overall, results provided support to manage fragmented populations of L. dolabelloides in the Tennessee River drainage as two management units (MUs), but did not provide evidence for the existence of ESUs following published molecular criteria. ?? The Author 2005. Published by Oxford University Studies on behalf of The Malacological Society of London, all rights reserved.

Recent sedimentation and surface-water flow patterns on the flood plain of the North Fork Forked Deer River, Dyer County, Tennessee

USGS Publications Warehouse

Wolfe, W.J.; Diehl, T.H.

1993-01-01

Sedimentation in the 19th and 20th centuries has had a major effect on surface-water drainage conditions along a 7-mile section of the North, Fork Forked Deer River flood plain, Dyer County, Tenn. During the century prior to 1930, 5 to 12 feet of sediment were deposited over much of the flood plain, resulting in channel obstruction and widespread flooding. The estimated bankfull capacity of the natural channel before it was channelized in 19 16 was comparable to the base flow of the river during the 1980's. Ditching of the river between 191i6 and 1;9,21 was followed by reductions in sedimentation rates over parts of the flood plain. However, the effects of sedimentation have persisted. Occlusions along the natural channel of the river have divided this stream reach into a series of sloughs. These sloughs continue to fill with sediment and are surrounded by ponds that have expanded since 1941. Degradation of the North Fork Forked Deer ditch may eventually reduce ponding over much of the flood plain. Active incision of headcuts in both banks of the ditch is enhancing the drainage of widespread ponded areas. These headcuts likely will have limited effect on drainage of most tributaries. The highest recent sedimentation rates, in places more than 0.2 foot per year, are concentrated near the flood-plain margin along tributary streams. In conjunction with beaver dams and debris, ongoing sedimentation has blocked flow in several tributaries, posing a flood hazard to agricultural land near the flood-plain margin. The occluded tributaries likely will continue to overflow unless they are periodically dredged or their sediment loads are reduced.

Oak Grove Fork Habitat Improvement Project, 1988 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bettin, Scott

The Lower Oak Grove Fork of the Clackamas River is a fifth-order tributary of the Clackamas River drainage supporting depressed runs of coho and chinook salmon, and summer and winter steelhead. Habitat condition rating for the Lower Oak Grove is good, but smelt production estimates are below the average for Clackamas River tributaries. Limiting factors in the 3.8 miles of the Lower Oak Grove supporting anadromous fish include an overall lack of quality spawning and rearing habitat. Beginning in 1986. measures to improve fish habitat in the Lower Oak Grove were developed in coordination with the Oregon Department of Fishmore » and Wildlife (ODF&W) and Portland General Electric (PGE) fisheries biologists. Prior to 1986, no measures had been applied to the stream to mitigate for PGE's storage and regulation of flows in the Oak Grove Fork (Timothy Lake, Harriet Lake). Catchable rainbow trout are stocked by ODF&W two or three times a year during the trout fishing season in the lowermost portion of the Oak Grove Fork near two Forest Service campgrounds (Ripplebrook and Rainbow). The 1987 field season marked the third year of efforts to improve fish habitat of the Lower Oak Grove Fork and restore anadromous fish production. The efforts included the development of an implementation plan for habitat improvement activities in the Lower Oak Grove Fork. post-project monitoring. and maintenance of the 1986 improvement structures. No new structures were constructed or placed in 1987. Fiscal year 1988 brought a multitude of changes which delayed implementation of plans developed in 1987. The most prominent change was the withdrawal of the proposed Spotted Owl Habitat Area (SOHA) which overlapped the Oak Grove project implementation area. Another was the change in the Forest Service biologist responsible for implementation and design of this project.« less

Dietary effects of metals-contaminated invertebrates from the Coeur d'Alene River, Idaho, on cutthroat trout

USGS Publications Warehouse

Farag, A.M.; Woodward, D.F.; Brumbaugh, W.; Goldstein, J.N.; MacConnell, Elizabeth; Hogstrand, Christer; Barrows, F.T.

1999-01-01

Benthic macroinvertebrates with elevated concentrations of metals were collected from the Coeur d'Alene (CDA) River, Idaho, pasteurized, and fed to cutthroat trout Oncorhynchus clarki in the laboratory from start of feeding until 90 d posthatch. Invertebrates were collected from two sites known to contain elevated concentrations of metals: near Pinehurst in the South Fork of the CDA River and at Cataldo, approximately 5 km below the confluence of the South Fork and the North Fork. Invertebrates collected from a relatively clean site in the North Fork were used as a reference diet. We performed measurements of fish health that indicate reduced fitness of fish fed the South Fork and Cataldo diets. Effects measured were reduced feeding activity, increased number of macrophage aggregates and hyperplasia of cells in the kidney, degeneration of mucosal epithelium in the pyloric caecae, and metallothionein induction. These effects would likely reduce growth and survival of fish in the wild. Vacuolization of glial cells were also observed in fish fed the Cataldo diet. Metals in the water often exacerbated the histological effects observed. Although the invertebrates collected near Cataldo had lower concentrations of arsenic (As), cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn) than the invertebrates from the South Fork, fish fed the Cataldo diet had equally high or higher concentrations of all metals except as by day 44. The Cataldo diet also caused the most deleterious effects on survival and growth. These findings are especially important for early life stage fish, whose diet consists wholly of benthic macroinvertebrates. Therefore, fish feeding on invertebrates in the CDA River below the Bunker Hill smelting complex are at risk of reduced fitness.

Use of a watershed-modeling approach to assess hydrologic effects of urbanization, North Fork Pheasant Branch basin near Middleton, Wisconsin

USGS Publications Warehouse

Steuer, Jeffrey J.; Hunt, R.J.

2001-01-01

The North Fork Pheasant Branch Basin in Dane County, Wisconsin is expected to undergo development. There are concerns that development will adversely affect water resources with increased flood peaks, increased runoff volumes, and increased pollutant loads. To provide a scientific basis for evaluating the hydrologic system response to development the Precipitation Runoff Modeling System (PRMS) was used to model the upper Pheasant Branch Creek watershed with an emphasis on the North Fork Basin. The upper Pheasant Branch Creek (18.3 mi2; 11,700 acres) Basin was represented with 21 Hydrologic Response Units (daily time step) and 50 flow planes (5-minute time steps). Precipitation data from the basin outlet streamflow-gaging station located at Highway 12 and temperature data from a nearby airport were used to drive the model. Continuous discharge records at three gaging stations were used for model calibration. To qualitatively assess model representation of small subbasins, periodic reconnaissance, often including a depth measurement, was made after precipitation to determine the occurrence of flow in ditches and channels from small subbasins. As a further effort to verify the model on a small subbasin scale, continuous-stage sensors (15-minute intervals) measured depth at the outlets of three small subbasins (500 to 1,200 acres). Average annual precipitation for the simulation period from 1993 to 1998 was 35.2 inches. The model simulations showed that, on average, 23.9 inches were intercepted by vegetation, or lost to evapotranspiration, 6.0 inches were infiltrated and moved to the regional ground-water system, and 4.8 inches contributed to the upper Pheasant Branch streamflow. The largest runoff event during the calibration interval was in July 1993 (746 ft3/sec; with a recurrence interval of approximately 25 years). Resulting recharge rates from the calibrated model were subsequently used as input into a ground-water-flow model. Average annual recharge varied spatially from 2.3 inches per year in the highly impervious commercial/industrial area to 9.7 inches per year in the undeveloped North Fork Basin with an average overall recharge rate of 8.1 inches per year. Two development scenarios were examined to assess changes in water-budget fluxes. In scenario A, when development was predominantly low-density residential with 5 to 10 percent commercial development along principal roadways, mean annual streamflow increased by 53 percent, overland flow increased by 84 percent, base flow decreased by 15 percent and annual recharge to the regional ground-water system was reduced by 10 percent. In development scenario B, the entire North Fork and intervening area basins contained 50 percent commercial and 50 percent medium density residential land use. Annual storm runoff increased by over 450 percent. The ground-water model for the Pheasant Branch that used the scenario B recharge rates simulated a lowered water table with zero base flow and that flow from Frederick Springs would be reduced 26 percent from present-day (1993?98) conditions.An additional example application of the model evaluated locations of flood detention ponds and potential recharge areas that may mitigate the changes in flood peaks and ground-water recharge resulting from urbanization. From February 1998 through July 1998, water-quality samples were collected by use of stage-activated automated samplers. Median suspended- sediment concentrations were similar between the North and South Fork Basins (194 and 242 mg/L, respectively); however, for other constituents, North Fork values were considerably higher: median phosphorus concentrations by 4 times (1.5 and 0.35 mg/L), median ammonia concentrations by 13 times (1.9 and 0.14 mg/L), and the phosphorus-to-sediment ratio by more than 6 times (21 and 3.1 mg/g).

76 FR 13172 - Placer County Water Agency

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-10

... Water Agency Notice of Application Tendered for Filing with the Commission and Establishing Procedural... County Water Agency e. Name of Project: Middle Fork American River Project f. Location: The Middle Fork...) h. Applicant Contact: Andy Fecko, Project Manager, Placer County Water Agency, 144 Ferguson Road...

PLACING TOP CHORD CENTER PANEL. View is to the northwest ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PLACING TOP CHORD CENTER PANEL. View is to the northwest from the old suspension bridge. Chord members being moved into place by jigger stick and highline - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

19. Heat Pump, view to the southwest. This system provides ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. Heat Pump, view to the southwest. This system provides ventilation air heating and cooling throughout the powerhouse. - Washington Water Power Clark Fork River Noxon Rapids Hydroelectric Development, Powerhouse, South bank of Clark Fork River at Noxon Rapids, Noxon, Sanders County, MT

Superfluidity of 4He in dense aerogel studied using quartz tuning fork

NASA Astrophysics Data System (ADS)

Matsumoto, K.; Okamoto, R.; Nakajima, A.; Abe, S.

2018-03-01

Superfluid 4He in aerogel is of interest because it has a normal component coupling to gel strand due to viscosity and a superfluid component with zero viscosity. Superfluid helium in aerogel has two sound modes, a slow critical mode and a fast one. In this study, quartz tuning fork was used in order to study acoustic properties of liquid 4He in aerogel with 90% porosity. Two pieces of aerogel were glued on both prongs of quartz tuning fork that had a resonance frequency of 33 kHz. The tuning fork was immersed in liquid 4He from 2 to 20 bar. The resonance frequency increased in the superfluid phase due to decrease in loaded mass. Temperature variation of resonance frequency was explained by that of superfluid density. Superfluid transition in aerogel was 2 mK lower than that without gel. Additional dissipation was observed in the temperature range between 1 K and transition temperature.

77 FR 23059 - Endangered and Threatened Wildlife and Plants; Determination of Endangered Status for Three Forks...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-17

...We, the U.S. Fish and Wildlife Service (Service), determine endangered status for the Three Forks springsnail (Pyrgulopsis trivialis) and threatened status for the San Bernardino springsnail (Pyrgulopsis bernardina); and designate critical habitat for both species under the Endangered Species Act of 1973, as amended (Act). In total, approximately 17.2 acres (6.9 hectares) are designated as critical habitat for Three Forks springsnail in Apache County, Arizona, and approximately 2.0 acres (0.8 hectares) for San Bernardino springsnail in Cochise County, Arizona. This final rule implements the Federal protections provided by the Act for these species.

Real-Time Ozone Detection Based on a Microfabricated Quartz Crystal Tuning Fork Sensor

PubMed Central

Wang, Rui; Tsow, Francis; Zhang, Xuezhi; Peng, Jhih-Hong; Forzani, Erica S.; Chen, Yongsheng; Crittenden, John C.; Destaillats, Hugo; Tao, Nongjian

2009-01-01

A chemical sensor for ozone based on an array of microfabricated tuning forks is described. The tuning forks are highly sensitive and stable, with low power consumption and cost. The selective detection is based on the specific reaction of the polymer with ozone. With a mass detection limit of ∼2 pg/mm2 and response time of 1 second, the sensor coated with a polymer sensing material can detect ppb-level ozone in air. The sensor is integrated into a miniaturized wearable device containing a detection circuit, filtration, battery and wireless communication chip, which is ideal for personal and microenvironmental chemical exposure monitoring. PMID:22346720

LOOKING EAST. View is to the northeast along centerline of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOOKING EAST. View is to the northeast along centerline of deck, looking from Humboldt County toward Trinity County. The old suspension bridge is still in place at right - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

77 FR 61656 - Petition for Waiver of Compliance

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-10

... County Heritage Foundation, Inc. (MCHF), on behalf of the Big South Fork Scenic Railway (BSFSRY), has... on the historic Kentucky & Tennessee Railway into the river gorge of the Big South Fork of the... in these proceedings by submitting written views, data, or comments. FRA does not anticipate...

Development of a Flood-Warning System and Flood-Inundation Mapping in Licking County, Ohio

DOT National Transportation Integrated Search

2012-08-01

Digital flood-inundation maps for selected reaches of South Fork Licking River, Raccoon Creek, North Fork Licking River, and the Licking River in Licking County, Ohio, were created by the U.S. Geological Survey (USGS), in cooperation with the Ohio De...

East Fork Watershed Cooperative Meeting: Local Representatives Briefing

EPA Science Inventory

USEPA research in the East Fork of the Little Miami River Watershed takes a whole system approach to determining how to best manage water quality in this large multi-use watershed. The success of the research relies on effective partnerships with other stakeholders of water quali...

Geology, Streamflow, and Water Chemistry of the Talufofo Stream Basin, Saipan, Northern Mariana Islands

USGS Publications Warehouse

Izuka, Scot K.; Ewart, Charles J.

1995-01-01

A study of the geology, streamflow, and water chemistry of Talufofo Stream Basin, Saipan, Commonwealth of the Northern Mariana Islands, was undertaken to determine the flow characteristics of Talufofo Stream and the relation to the geology of the drainage basin. The Commonwealth government is exploring the feasibility of using water from Talufofo Stream to supplement Saipan's stressed municipal water supply. Streamflow records from gaging stations on the principal forks of Talufofo Stream indicate that peak streamflows and long-term average flow are higher at the South Fork gaging station than at the Middle Fork gaging station because the drainage area of the South Fork gaging station is larger, but persistent base flow from ground-water discharge during dry weather is greater in the Middle Fork gaging station. The sum of the average flows at the Middle Fork and South Fork gaging stations, plus an estimate of the average flow at a point in the lower reaches of the North Fork, is about 2.96 cubic feet per second or 1.91 million gallons per day. Although this average represents the theoretical maximum long-term draft rate possible from the Talufofo Stream Basin if an adequate reservoir can be built, the actual amount of surface water available will be less because of evaporation, leaks, induced infiltration, and reservoir-design constraints. Base-flow characteristics, such as stream seepage and spring discharge, are related to geology of the basin. Base flow in the Talufofo Stream Basin originates as discharge from springs near the base of limestones located in the headwaters of Talufofo Stream, flows over low-permeability volcanic rocks in the middle reaches, and seeps back into the high-permeability limestones in the lower reaches. Water sampled from Talufofo Stream during base flow had high dissolved-calcium concentrations (between 35 and 98 milligrams per liter), characteristic of water from a limestone aquifer. Concentrations of potassium, sodium, and chloride ions in water samples from Talufofo Stream are characteristic of water draining a heavily vegetated basin near the ocean. The streamflow and water-chemistry data indicate that discharge from springs is in hydraulic connection with the limestone aquifer near the headwaters of the basin. The base flow therefore is subject to stresses placed on the nearby limestone ground-water system. Pumping from wells in the limestones at the headwaters of Talufofo Stream Basin may decrease spring flow in Talufofo Stream.

Hydrogeology and simulated groundwater flow and availability in the North Fork Red River aquifer, southwest Oklahoma, 1980–2013

USGS Publications Warehouse

Smith, S. Jerrod; Ellis, John H.; Wagner, Derrick L.; Peterson, Steven M.

2017-09-28

On September 8, 1981, the Oklahoma Water Resources Board established regulatory limits on the maximum annual yield of groundwater (343,042 acre-feet per year) and equal-proportionate-share (EPS) pumping rate (1.0 acre-foot per acre per year) for the North Fork Red River aquifer. The maximum annual yield and EPS were based on a hydrologic investigation that used a numerical groundwater-flow model to evaluate the effects of potential groundwater withdrawals on groundwater availability in the North Fork Red River aquifer. The Oklahoma Water Resources Board is statutorily required (every 20 years) to update the hydrologic investigation on which the maximum annual yield and EPS were based. Because 20 years have elapsed since the final order was issued, the U.S. Geological Survey, in cooperation with the Oklahoma Water Resources Board, conducted an updated hydrologic investigation and evaluated the effects of potential groundwater withdrawals on groundwater flow and availability in the North Fork Red River aquifer in Oklahoma. This report describes a hydrologic investigation of the North Fork Red River aquifer that includes an updated summary of the aquifer hydrogeology. As part of this investigation, groundwater flow and availability were simulated by using a numerical groundwater-flow model.The North Fork Red River aquifer in Beckham, Greer, Jackson, Kiowa, and Roger Mills Counties in Oklahoma is composed of about 777 square miles (497,582 acres) of alluvium and terrace deposits along the North Fork Red River and tributaries, including Sweetwater Creek, Elk Creek, Otter Creek, and Elm Fork Red River. The North Fork Red River is the primary source of surface-water inflow to Lake Altus, which overlies the North Fork Red River aquifer. Lake Altus is a U.S. Bureau of Reclamation reservoir with the primary purpose of supplying irrigation water to the Lugert-Altus Irrigation District.A hydrogeologic framework was developed for the North Fork Red River aquifer and included a definition of the aquifer extent and potentiometric surface, as well as a description of the textural and hydraulic properties of aquifer materials. The hydrogeologic framework was used in the construction of a numerical groundwater-flow model of the North Fork Red River aquifer described in this report. A conceptual model of aquifer inflows and outflows was developed for the North Fork Red River aquifer to constrain the construction and calibration of a numerical groundwater-flow model that reasonably represented the groundwater-flow system. The conceptual-model water budget estimated mean annual inflows to and outflows from the North Fork Red River aquifer for the period 1980–2013 and included a sub-accounting of mean annual inflows and outflows for the portions of the aquifer that were upgradient and downgradient from Lake Altus. The numerical groundwater-flow model simulated the period 1980–2013 and was calibrated to water-table-altitude observations at selected wells, monthly base flow at selected streamgages, net streambed seepage as estimated for the conceptual model, and Lake Altus stage.Groundwater-availability scenarios were performed by using the calibrated numerical groundwater-flow model to (1) estimate the EPS pumping rate that guarantees a minimum 20-, 40-, and 50-year life of the aquifer, (2) quantify the potential effects of projected well withdrawals on groundwater storage over a 50-year period, and (3) simulate the potential effects of a hypothetical (10-year) drought on base flow and groundwater storage. The results of the groundwater-availability scenarios could be used by the Oklahoma Water Resources Board to reevaluate the maximum annual yield of groundwater from the North Fork Red River aquifer.EPS scenarios for the North Fork Red River aquifer were run for periods of 20, 40, and 50 years. The 20-, 40-, and 50-year EPS pumping rates under normal recharge conditions were 0.59, 0.52, and 0.52 acre-foot per acre per year, respectively. Given the 497,582-acre aquifer area, these rates correspond to annual yields of about 294,000, 259,000, and 259,000 acre-feet per year, respectively. Groundwater storage at the end of the 20-year EPS scenario was about 951,000 acre-feet, or about 1,317,000 acre-feet (58 percent) less than the starting EPS scenario storage. This decrease in storage was equivalent to a mean water-level decline of about 22 feet. Most areas of the active alluvium near the North Fork Red River, Elk Creek, and Elm Fork Red River remained partially saturated through the end of the EPS scenario because of streambed seepage. Lake Altus storage was reduced to zero after 6–7 years of EPS pumping in each scenario.Projected 50-year pumping scenarios were used to simulate the effects of selected well withdrawal rates on groundwater storage of the North Fork Red River aquifer and base flows in the North Fork Red River upstream from Lake Altus. The effects of well withdrawals were evaluated by comparing changes in groundwater storage and base flow between four 50-year scenarios using (1) no groundwater pumping, (2) mean pumping rates for the study period (1980–2013), (3) 2013 pumping rates, and (4) increasing demand pumping rates. The increasing demand pumping rates assumed a 20.4-percent increase in pumping over 50 years based on 2010–60 demand projections for southwest Oklahoma.Groundwater storage after 50 years with no pumping was about 2,606,000 acre-feet, or 137,000 acre-feet (5.5 percent) greater than the initial groundwater storage; this groundwater storage increase is equivalent to a mean water-level increase of 2.3 feet. Groundwater storage after 50 years with the mean pumping rate for the study period (1980–2013) was about 2,476,000 acre-feet, or about 7,000 acre-feet (0.3 percent) greater than the initial groundwater storage; this groundwater storage increase is equivalent to a mean water-level increase of 0.1 foot. Groundwater storage at the end of the 50-year period with 2013 pumping rates was about 2,398,000 acre-feet, or about 70,000 acre-feet (2.8 percent) less than the initial storage; this groundwater storage decrease is equivalent to a mean water-level decline of 1.2 feet. Groundwater storage at the end of the 50-year period with increasing demand pumping rates was about 2,361,000 acre-feet, or about 107,000 acre-feet (4.3 percent) less than the initial storage; this groundwater storage decrease is equivalent to a mean water-level decline of 1.8 feet. Mean annual base flow simulated at the Carter streamgage (07301500) on North Fork Red River increased by about 4,000 acre-feet (10 percent) after 50 years with no pumping and decreased by about 5,400 acre-feet (13 percent) after 50 years with increasing demand pumping rates. Mean annual base flow simulated at the North Fork Red River inflow to Lake Altus increased by about 7,400 acre-feet (15 percent) after 50 years with no pumping and decreased by about 5,800 acre-feet (12 percent) after 50 years with increasing demand pumping rates.A hypothetical 10-year drought scenario was used to simulate the effects of a prolonged period of reduced recharge on groundwater storage and Lake Altus stage and storage. Drought effects were quantified by comparing the results of the drought scenario to those of the calibrated numerical model (no drought). To simulate the hypothetical drought, recharge in the calibrated numerical model was reduced by 50 percent during the simulated drought period (1984–1993). Groundwater storage at the end of the drought period was about 2,271,000 acre-feet, or about 426,000 acre-feet (15.8 percent) less than the groundwater storage of the calibrated numerical model. This decrease in groundwater storage is equivalent to a mean water-table-altitude decline of 7.1 feet. At the end of the 10-year hypothetical drought period, base flows at the Sweetwater (07301420), Carter (07301500), Headrick (07305000), and Snyder (07307010) streamgages had decreased by about 37, 61, 44, and 45 percent, respectively. The minimum Lake Altus storage simulated during the drought period was 403 acre-feet, which was a decline of 92 percent from the nondrought storage. Reduced base flows in the North Fork Red River were the primary cause of Lake Altus storage declines.

E-Time for Learning

ERIC Educational Resources Information Center

Fawson, Peter; Peterson, Troy

2011-01-01

For the past three years, the faculty and administrators of Diamond Fork Junior High School--a large junior high in Spanish Fork, Utah--have been on an amazing journey of examining the school's educational practices with the intent of improving learning opportunities for all its students. In March 2009, the administrators and the faculty…

LIFE HISTORY MONITORING OF SALMONIDS IN THE WEST FORK SMITH RIVER, UMPQUA BASIN, OREGON

EPA Science Inventory

As a life-cycle monitoring basin for the Oregon Salmon Plan, the Oregon Department of Fish and Wildlife has estimated adult returns, distribution and smolt outmigration of coho, chinook and winter steelhead in the West Fork Smith River since 1998. In 2001/2002, the Environmenta...

East Fork Watershed Cooperative: Toward better system-scale watershed management

EPA Science Inventory

The East Fork Watershed Cooperative is a group intent on understanding how to best manage water quality in a large mixed-use Midwestern watershed system. The system contains a reservoir that serves as a source of drinking water and is popular for water recreation. The reservoir i...

Final Environmental Assessment Demolition of Alpha Ramp, Grand Forks Air Force Base, North Dakota

DTIC Science & Technology

2007-01-01

including spring wheat, barley, sunflowers, potatoes, and sugar beets. Uncultivated lands are generally used for pasture and hay, urban...Forks, ND 58203 Jack HOURS; Adul* Mon.-Sat. 12 noon - 6pm Main www.gfpets.com (next to Dairy Queen) Emerado, ND 58228 * Competitive prices & Monthly

Technical Assessment of Roaring Fork Transit Authority ITS / Related Alternative Transportation Concepts on the White River National Forest.

DOT National Transportation Integrated Search

2007-12-12

At the request of the U.S. Department of Agriculture Forest Service (USFS), the U.S. DOT : Volpe Center conducted a review of the status of Intelligent Transportation Systems (ITS) : planning by the Roaring Fork Transit Authority (RFTA). The assessme...

STARTING EXCAVATION PIER 2. This view is roughly northeast, with ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

STARTING EXCAVATION PIER 2. This view is roughly northeast, with Pier 2 on the Trinity County end of the bridge. The old suspension bridge, at upper right, was upstream of new bridge - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

"Hydrologic effects of forest harvest in northwestern California, USA"

Treesearch

Robert Ziemer

2000-01-01

Streamflow, suspended sediment, and bedload have been monitored since 1962 in the 473-ha North Fork and 424-ha South Fork of Caspar Creek. These watersheds are about 7 km from the Pacific Ocean, on the Jackson Demonstation State Forest, 10 km south of Fort Bragg, California, USA

131. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

131. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN FALLS COUNTY, SOUTH OF HANSEN, IDAHO; INLET SIDE OF LOW LINE CANAL, WEST VIEW. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

Final report from VFL Technologies for the pilot-scale thermal treatment of lower East Fork Poplar Creek floodplain soils. LEFPC appendices, Volume 4, Appendix V-C

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1994-09-01

This is the the final verification run data package for pilot scale thermal treatment of lower East Fork Poplar Creek floodplain soils. Included are data on volatiles, semivolatiles, and TCLP volatiles.

133. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

133. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN FALLS COUNTY, SOUTH OF HANSEN, IDAHO; VIEW OF OUTLET SIDE OF LOW LINE GATES. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

27 CFR 9.112 - Arkansas Mountain.

Code of Federal Regulations, 2010 CFR

2010-04-01

.... (2) Fort Smith, Arkansas-Oklahoma, 1:250,000 series, 1978. (c) Boundary—(1) General. The Arkansas... converges with the Arkansas River, near Yoestown, Arkansas (or the Fort Smith map), the boundary proceeds... county line to Jones Fork (on the Fort Smith map). (xvi) Then southward along Jones Fork until it joins...

Development of a Flood-Warning System and Flood-Inundation Mapping in Licking County, Ohio : Executive Summary Report

DOT National Transportation Integrated Search

2012-04-01

Licking County, Ohio, has experienced numerous floods with the majority of flood damages occurring in the central and south-central areas of the county along four streams: the Licking River, North Fork Licking River, South Fork Licking River, and Rac...

NUTRIENT CONCENTRATIONS IN FLOWING WATERS OF THE SOUTH FORK BROAD RIVER, GEORGIA WATERSHED

EPA Science Inventory

We monitored concentrations of nutrients, dissolved organic matter (DOM) and other parameters in 17 headwater streams, at three sites on the main stem, and in three major tributaries near their confluence with the South Fork Broad River on a monthly basis for over a year. Concent...

NUTRIENT CONCENTRATIONS IN FLOWING WATERS OF THE SOUTH FORK BROAD RIVER, GEORGIA WATERSHED

EPA Science Inventory

The South Fork Broad River (SFBR) drains about 635 km2 of the Georgia Piedmont. The SFBR watershed is primarily rural and undeveloped although the human population increased by about 25% between 1990 and 2000. Forestry and agriculture are the main land uses. Agriculture consis...

36 CFR 261.75 - Regulations applicable to Region 5, California Region, as defined in § 200.2.

Code of Federal Regulations, 2013 CFR

2013-07-01

...: (1) Middle Fork of the Feather River means the river and land area in or adjacent to Plumas National... equipment in the wild river zone of the Middle Fork of the Feather River, except on the Stag Point Trail or...

36 CFR 261.75 - Regulations applicable to Region 5, California Region, as defined in § 200.2.

Code of Federal Regulations, 2010 CFR

2010-07-01

...: (1) Middle Fork of the Feather River means the river and land area in or adjacent to Plumas National... equipment in the wild river zone of the Middle Fork of the Feather River, except on the Stag Point Trail or...

36 CFR 261.75 - Regulations applicable to Region 5, California Region, as defined in § 200.2.

Code of Federal Regulations, 2012 CFR

2012-07-01

...: (1) Middle Fork of the Feather River means the river and land area in or adjacent to Plumas National... equipment in the wild river zone of the Middle Fork of the Feather River, except on the Stag Point Trail or...

36 CFR 261.75 - Regulations applicable to Region 5, California Region, as defined in § 200.2.

Code of Federal Regulations, 2011 CFR

2011-07-01

...: (1) Middle Fork of the Feather River means the river and land area in or adjacent to Plumas National... equipment in the wild river zone of the Middle Fork of the Feather River, except on the Stag Point Trail or...

36 CFR 261.75 - Regulations applicable to Region 5, California Region, as defined in § 200.2.

Code of Federal Regulations, 2014 CFR

2014-07-01

...: (1) Middle Fork of the Feather River means the river and land area in or adjacent to Plumas National... equipment in the wild river zone of the Middle Fork of the Feather River, except on the Stag Point Trail or...

13. Greasing Pump and Governor Accumulator Tank Compressors, view to ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Greasing Pump and Governor Accumulator Tank Compressors, view to the west. The greasing pump, visible in left foreground, services all four turbine pits. - Washington Water Power Clark Fork River Cabinet Gorge Hydroelectric Development, Powerhouse, North Bank of Clark Fork River at Cabinet Gorge, Cabinet, Bonner County, ID

78 FR 78995 - Proposed Flood Hazard Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-27

... resolution process. SRPs are independent panels of experts in hydrology, hydraulics, and other pertinent..., Fountain City, IN 47341. Town of Greens Fork Town Hall, 12 South Water Street, Greens Fork, IN 47345. Town... Office, 212 Water Street, Lyons, MI 48851. Village of Muir Village Hall, 122 Superior Street, Muir, MI...

How Clean Is the River?

ERIC Educational Resources Information Center

Schaaf, Sherry

2005-01-01

On the Olympic Peninsula of Washington State, salmon and water quality are more than just a news story. They are vitally important to the economy and environment--and are the inspiration for environmental science learning adventures. In this water quality project, four third-grade classes from Forks Elementary School in Forks, Washington,…