Genetic diversity and population structure of Vibrio cholerae.

PubMed

Beltrán, P; Delgado, G; Navarro, A; Trujillo, F; Selander, R K; Cravioto, A

1999-03-01

Multilocus enzyme electrophoresis (MLEE) of 397 Vibrio cholerae isolates, including 143 serogroup reference strains and 244 strains from Mexico and Guatemala, identified 279 electrophoretic types (ETs) distributed in two major divisions (I and II). Linkage disequilibrium was demonstrated in both divisions and in subdivision Ic of division I but not in subdivision Ia, which includes 76% of the ETs. Despite this evidence of relatively frequent recombination, clonal lineages may persist for periods of time measured in at least decades. In addition to the pandemic clones of serogroups O1 and O139, which form a tight cluster of four ETs in subdivision Ia, MLEE analysis identified numerous apparent clonal lineages of non-O1 strains with intercontinental distributions. A clone of serogroup O37 that demonstrated epidemic potential in the 1960s is closely related to the pandemic O1/O139 clones, but the nontoxigenic O1 Inaba El Tor reference strain is not. A strain of serogroup O22, which has been identified as the most likely donor of exogenous rfb region DNA to the O1 progenitor of the O139 clone, is distantly related to the O1/O139 clones. The close evolutionary relationships of the O1, O139, and O37 epidemic clones indicates that new cholera clones are likely to arise by the modification of a lineage that is already epidemic or is closely related to such a clone.

Programing Procedures Manual (PPM).

DTIC Science & Technology

1981-12-15

terms ’reel’, ’unit’, and ’volume’ are synonymous and completely interchangeable in the CLOSE statement. Treatment of sequential mass storage files is...logically equivalent to the treatment of a file on tape or analogous sequential media. * For the purpose of showing the effect of various types of CLOSE...Overlay Area CA6 Address of Abend Relative to beginning of overlay segment The programer can now refer to the compile source listing for the overlay

Genome Sequencing of Steroid Producing Bacteria Using Ion Torrent Technology and a Reference Genome.

PubMed

Sola-Landa, Alberto; Rodríguez-García, Antonio; Barreiro, Carlos; Pérez-Redondo, Rosario

2017-01-01

The Next-Generation Sequencing technology has enormously eased the bacterial genome sequencing and several tens of thousands of genomes have been sequenced during the last 10 years. Most of the genome projects are published as draft version, however, for certain applications the complete genome sequence is required.In this chapter, we describe the strategy that allowed the complete genome sequencing of Mycobacterium neoaurum NRRL B-3805, an industrial strain exploited for steroid production, using Ion Torrent sequencing reads and the genome of a close strain as the reference. This protocol can be applied to analyze the genetic variations between closely related strains; for example, to elucidate the point mutations between a parental strain and a random mutagenesis-derived mutant.

Things to Consider If You Have MG and Are Thinking about Getting Pregnant

MedlinePlus

... diagnosis and optimal care of individuals affected by myasthenia gravis and closely related disorders and to improve their ... the authors, but not necessarily those of the Myasthenia Gravis Foundation of America (MGFA). Any reference to a ...

The Relational Humor Inventory: Functions of Humor in Close Relationships.

ERIC Educational Resources Information Center

DeKoning, E.; Weiss, R. L.

2002-01-01

This study describes the development of a self-report measure of functional humor in relationships. People were asked to report on their own and their partner's use of humor in the marriage. The Relational Humor Inventory proved to be a useful instrument for tapping important positive and negative relationship behaviors. (Contains 30 references, 4…

Beyond left and right: Automaticity and flexibility of number-space associations.

PubMed

Antoine, Sophie; Gevers, Wim

2016-02-01

Close links exist between the processing of numbers and the processing of space: relatively small numbers are preferentially associated with a left-sided response while relatively large numbers are associated with a right-sided response (the SNARC effect). Previous work demonstrated that the SNARC effect is triggered in an automatic manner and is highly flexible. Besides the left-right dimension, numbers associate with other spatial response mappings such as close/far responses, where small numbers are associated with a close response and large numbers with a far response. In two experiments we investigate the nature of this association. Associations between magnitude and close/far responses were observed using a magnitude-irrelevant task (Experiment 1: automaticity) and using a variable referent task (Experiment 2: flexibility). While drawing a strong parallel between both response mappings, the present results are also informative with regard to the question about what type of processing mechanism underlies both the SNARC effect and the association between numerical magnitude and close/far response locations.

ALTERED HISTOLOGY OF THE THYMUS AND SPLEEN IN CONTAMINANT-EXPOSED JUVENILE AMERICAN ALLIGATORS

EPA Science Inventory

Morphological difference in spleen and thymus are closely related to functional immune differences. Hormonal regulation of the immune system has been demonstrated in reptilian splenic and thymic tissue. Spleens and thymus were obtained from juvenile alligators at two reference si...

Multiple scattering corrections to the Beer-Lambert law. 2: Detector with a variable field of view.

PubMed

Zardecki, A; Tam, W G

1982-07-01

The multiple scattering corrections to the Beer-Lambert law in the case of a detector with a variable field of view are analyzed. We introduce transmission functions relating the received radiant power to reference power levels relevant to two different experimental situations. In the first case, the transmission function relates the received power to a reference power level appropriate to a nonattenuating medium. In the second case, the reference power level is established by bringing the receiver to the close-up position with respect to the source. To examine the effect of the variation of the detector field of view the behavior of the gain factor is studied. Numerical results modeling the laser beam propagation in fog, cloud, and rain are presented.

Reference equations of motion for automatic rendezvous and capture

NASA Technical Reports Server (NTRS)

Henderson, David M.

1992-01-01

The analysis presented in this paper defines the reference coordinate frames, equations of motion, and control parameters necessary to model the relative motion and attitude of spacecraft in close proximity with another space system during the Automatic Rendezvous and Capture phase of an on-orbit operation. The relative docking port target position vector and the attitude control matrix are defined based upon an arbitrary spacecraft design. These translation and rotation control parameters could be used to drive the error signal input to the vehicle flight control system. Measurements for these control parameters would become the bases for an autopilot or feedback control system (FCS) design for a specific spacecraft.

A Selected Bibliography.

ERIC Educational Resources Information Center

Planned Parenthood--World Population, New York, NY. Katherine Dexter McCormick Library.

The books in this bibliography have been chosen for those specialists and professionals in the fields of population and family planning who are seeking references either in their own disciplines or in closely related areas. Classification is based on that used by the Katharine Dexter McCormick Library. Annotated entries (182) are compiled under…

Determinants of Children's Health. Executive Summary.

ERIC Educational Resources Information Center

Grossman, Michael; And Others

This document consists of summaries of six closely-related studies which investigated empirically the determinants of children's health with particular reference to home and local environmental variables. The first study examined the relationship between a number of family characteristics and the health of white children (6 through 11 years of…

The Utility of Cognitive Plausibility in Language Acquisition Modeling: Evidence from Word Segmentation

ERIC Educational Resources Information Center

Phillips, Lawrence; Pearl, Lisa

2015-01-01

The informativity of a computational model of language acquisition is directly related to how closely it approximates the actual acquisition task, sometimes referred to as the model's "cognitive plausibility." We suggest that though every computational model necessarily idealizes the modeled task, an informative language acquisition…

Precise Electrochemical Drilling of Repeated Deep Holes

NASA Technical Reports Server (NTRS)

Kincheloe, J. P.

1985-01-01

Tooling enables maintenance of close tolerances. Tooling includes guide that holds electrochemical drilling electrodes in proper relative alinement and guide-positioning fixture clamps directly on reference surfaces of strut. High precision achieved by positioning tooling anew on each strut before drilling: Tolerances of (0.008 mm) maintained in some details.

Industrialization and Household Complexity in Rural Taiwan.

ERIC Educational Resources Information Center

Lavely, William

1990-01-01

In 274 Taiwanese townships, farm household complexity in 1960 and 1970 was positively related to the proportion of the labor force in nonagricultural occupations. The close proximity of industry to family farms in Taiwan has reduced rural to urban migration usually associated with industrialization. Contains 46 references. (Author/SV)

The session of the two dreams.

PubMed

Giannoni, Massimo

2009-02-01

Through the discussion of the clinical material the author tries to show how numerous aspects of traditional Jungian analysis are close to several theoretical and clinical developments of Relational Psychoanalysis. A short introduction about relational psychoanalysis is given. The relational aspects of Jungian theory and praxis are underlined. If we refer to these theoretical constructs, it becomes possible to work in an original way and think of the clinical setting as different from the classical Freudian one, without abandoning Jungian tradition.

Relations between normative beliefs and initiation intentions toward cigarette, alcohol and marijuana.

PubMed

Olds, R Scott; Thombs, Dennis L; Tomasek, Jennifer Ray

2005-07-01

To examine the relations between normative beliefs and intentions to initiate cigarette, alcohol, and marijuana use among adolescents reporting no prior use. An anonymous questionnaire was administered to 6,594 seventh- to twelfth-grade students in northeast Ohio. Separate analyses were conducted on sub-samples of respondents reporting no prior use of each substance. Within each of these 3 sub-samples, respondents were classified as holding high-risk intentions if they reported that they intended to begin using that particular substance within the next 6 months or were "not sure" of their intentions. Those reporting that they did not intend to start using a substance were classified as holding low-risk intentions. Multivariate logistic regression analyses examined the relations between normative beliefs and intention status (low- vs. high-risk), while accounting for socio-demographic characteristics. Across all 3 substances, normative beliefs were stronger predictors of intention status than socio-demographic variables. Higher levels of perceived acceptability and perceived prevalence were associated with holding high-risk intentions. Normative belief measures assessing close friend and sibling reference groups were much more important in explaining intention status than those assessing other reference groups (e.g., same age peers). Among adolescents with no prior use, normative beliefs concerning close friends and siblings may play an important role in the catalysis and support of intentions to initiate substance use. These findings challenge the utility of primary prevention strategies that provide normative feedback based on rates of substance use among distal reference groups.

New astronomical references in two Catalonian late medieval documents.

PubMed

Martínez, María José; Marco, Francisco J

2014-01-01

In 2008, after 13 years of preparation, the Generalitat of Catalunya finished the publication of the 10 volumes of the Dietaris de la Generalitat de Catalunya. The Dietaris, as well as a closely related source, the llibre de Jornades 1411/1484 de Jaume Safont, cover the period of 1411 to 1539. In this article, we examine astronomical references contained in these two sources, and place them in their historical context. Our main focus lies on astronomical phenomena that have not previously been published in the astronomical literature. In fact, relatively few astronomical records are accessible in Spanish medieval and early modern history, and our paper intends to fill this gap partially.

Scientifically Based Research and Peer-Reviewed Research under the IDEA: The Legal Definitions, Applications, and Implications

ERIC Educational Resources Information Center

Zirkel, Perry A.; Rose, Tessie

2009-01-01

A systematic analysis of the references to "scientifically based research" (SBR) and closely related terms, such as "peer-reviewed research" (PRR), in the Individuals with Disabilities Education Act (IDEA) legislation, regulations, commentary, and case law reveal that SBR and its primary variants apply largely to state support…

Japanese Culture-Bound Disorders: The Relationship between "Taijin Kyofusho, Hikikomori," and Shame

ERIC Educational Resources Information Center

Cole, Levi Edward

2013-01-01

First conceptualized in the 1960s, the term culture-bound disorders refers to a classification of mental disorders or syndromes that are considered specific or closely related to cultural factors and or particular ethnocultural groups. In Japan, two culture-bound disorders, "taijin kyofusho" and "hikikomori," have seized the…

Let's GO Tech to Pursue Sustainability and Happiness

ERIC Educational Resources Information Center

Lee, Lung-Sheng

2012-01-01

Technology refers to making or doing things to extend human capability to meet our needs or wants. Both technology and engineering are closely related. The majority of faculty and students in both Kisarazu National College of Technology (Kisarazu Kosen or KNCT) and National United University (NUU) are in the field of engineering. Based upon a…

Food Fussiness and Food Neophobia Share a Common Etiology in Early Childhood

ERIC Educational Resources Information Center

Smith, Andrea D.; Herle, Moritz; Fildes, Alison; Cooke, Lucy; Steinsbekk, Silje; Llewellyn, Clare H.

2017-01-01

Background: "Food fussiness" (FF) is the tendency to be highly selective about which foods one is willing to eat, and emerges in early childhood; "food neophobia" (FN) is a closely related characteristic but specifically refers to rejection of unfamiliar food. These behaviors are associated, but the extent to which their…

Self-Esteem: Issues for Adolescents with Chronic Illnesses and Disabilities. CYDLINE Reviews.

ERIC Educational Resources Information Center

Minnesota Univ., Minneapolis. National Center for Youth with Disabilities.

This annotated bibliography focuses on issues of self-esteem in adolescents with disabilities, which is seen as closely related to development of critical social skills. References have been drawn from the National Center for Youth with Disabilities' National Resource Library, a database about youth with chronic illnesses and disabilities. The…

ABACAS: algorithm-based automatic contiguation of assembled sequences

PubMed Central

Assefa, Samuel; Keane, Thomas M.; Otto, Thomas D.; Newbold, Chris; Berriman, Matthew

2009-01-01

Summary: Due to the availability of new sequencing technologies, we are now increasingly interested in sequencing closely related strains of existing finished genomes. Recently a number of de novo and mapping-based assemblers have been developed to produce high quality draft genomes from new sequencing technology reads. New tools are necessary to take contigs from a draft assembly through to a fully contiguated genome sequence. ABACAS is intended as a tool to rapidly contiguate (align, order, orientate), visualize and design primers to close gaps on shotgun assembled contigs based on a reference sequence. The input to ABACAS is a set of contigs which will be aligned to the reference genome, ordered and orientated, visualized in the ACT comparative browser, and optimal primer sequences are automatically generated. Availability and Implementation: ABACAS is implemented in Perl and is freely available for download from http://abacas.sourceforge.net Contact: sa4@sanger.ac.uk PMID:19497936

Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding.

PubMed

Versteirt, V; Nagy, Z T; Roelants, P; Denis, L; Breman, F C; Damiens, D; Dekoninck, W; Backeljau, T; Coosemans, M; Van Bortel, W

2015-03-01

Since its introduction in 2003, DNA barcoding has proven to be a promising method for the identification of many taxa, including mosquitoes (Diptera: Culicidae). Many mosquito species are potential vectors of pathogens, and correct identification in all life stages is essential for effective mosquito monitoring and control. To use DNA barcoding for species identification, a reliable and comprehensive reference database of verified DNA sequences is required. Hence, DNA sequence diversity of mosquitoes in Belgium was assessed using a 658 bp fragment of the mitochondrial cytochrome oxidase I (COI) gene, and a reference data set was established. Most species appeared as well-supported clusters. Intraspecific Kimura 2-parameter (K2P) distances averaged 0.7%, and the maximum observed K2P distance was 6.2% for Aedes koreicus. A small overlap between intra- and interspecific K2P distances for congeneric sequences was observed. Overall, the identification success using best match and the best close match criteria were high, that is above 98%. No clear genetic division was found between the closely related species Aedes annulipes and Aedes cantans, which can be confused using morphological identification only. The members of the Anopheles maculipennis complex, that is Anopheles maculipennis s.s. and An. messeae, were weakly supported as monophyletic taxa. This study showed that DNA barcoding offers a reliable framework for mosquito species identification in Belgium except for some closely related species. © 2014 John Wiley & Sons Ltd.

Automatic force balance calibration system

NASA Technical Reports Server (NTRS)

Ferris, Alice T. (Inventor)

1995-01-01

A system for automatically calibrating force balances is provided. The invention uses a reference balance aligned with the balance being calibrated to provide superior accuracy while minimizing the time required to complete the calibration. The reference balance and the test balance are rigidly attached together with closely aligned moment centers. Loads placed on the system equally effect each balance, and the differences in the readings of the two balances can be used to generate the calibration matrix for the test balance. Since the accuracy of the test calibration is determined by the accuracy of the reference balance and current technology allows for reference balances to be calibrated to within +/-0.05% the entire system has an accuracy of +/-0.2%. The entire apparatus is relatively small and can be mounted on a movable base for easy transport between test locations. The system can also accept a wide variety of reference balances, thus allowing calibration under diverse load and size requirements.

Automatic force balance calibration system

NASA Technical Reports Server (NTRS)

Ferris, Alice T. (Inventor)

1996-01-01

A system for automatically calibrating force balances is provided. The invention uses a reference balance aligned with the balance being calibrated to provide superior accuracy while minimizing the time required to complete the calibration. The reference balance and the test balance are rigidly attached together with closely aligned moment centers. Loads placed on the system equally effect each balance, and the differences in the readings of the two balances can be used to generate the calibration matrix for the test balance. Since the accuracy of the test calibration is determined by the accuracy of the reference balance and current technology allows for reference balances to be calibrated to within .+-.0.05%, the entire system has an accuracy of a .+-.0.2%. The entire apparatus is relatively small and can be mounted on a movable base for easy transport between test locations. The system can also accept a wide variety of reference balances, thus allowing calibration under diverse load and size requirements.

Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis.

PubMed

Nguyen, Duc Quan; Eamens, Andrew L; Grof, Christopher P L

2018-01-01

Quantitative real-time polymerase chain reaction (RT-qPCR) is the key platform for the quantitative analysis of gene expression in a wide range of experimental systems and conditions. However, the accuracy and reproducibility of gene expression quantification via RT-qPCR is entirely dependent on the identification of reliable reference genes for data normalisation. Green foxtail ( Setaria viridis ) has recently been proposed as a potential experimental model for the study of C 4 photosynthesis and is closely related to many economically important crop species of the Panicoideae subfamily of grasses, including Zea mays (maize), Sorghum bicolor (sorghum) and Sacchurum officinarum (sugarcane). Setaria viridis (Accession 10) possesses a number of key traits as an experimental model, namely; (i) a small sized, sequenced and well annotated genome; (ii) short stature and generation time; (iii) prolific seed production, and; (iv) is amendable to Agrobacterium tumefaciens -mediated transformation. There is currently however, a lack of reference gene expression information for Setaria viridis ( S. viridis ). We therefore aimed to identify a cohort of suitable S. viridis reference genes for accurate and reliable normalisation of S. viridis RT-qPCR expression data. Eleven putative candidate reference genes were identified and examined across thirteen different S. viridis tissues. Of these, the geNorm and NormFinder analysis software identified SERINE / THERONINE - PROTEIN PHOSPHATASE 2A ( PP2A ), 5 '- ADENYLYLSULFATE REDUCTASE 6 ( ASPR6 ) and DUAL SPECIFICITY PHOSPHATASE ( DUSP ) as the most suitable combination of reference genes for the accurate and reliable normalisation of S. viridis RT-qPCR expression data. To demonstrate the suitability of the three selected reference genes, PP2A , ASPR6 and DUSP , were used to normalise the expression of CINNAMYL ALCOHOL DEHYDROGENASE ( CAD ) genes across the same tissues. This approach readily demonstrated the suitably of the three selected reference genes for the accurate and reliable normalisation of S. viridis RT-qPCR expression data. Further, the work reported here forms a highly useful platform for future gene expression quantification in S. viridis and can also be potentially directly translatable to other closely related and agronomically important C 4 crop species.

To Enter the Text as into a Dream: Tracing the Unconscious Effects of Reading Experience

ERIC Educational Resources Information Center

Lewkowich, David

2016-01-01

In this article, I suggest that, when readers speak about reading, the language they use is closely related to the modes and movements of speech employed by the dreamer recounting their dream. With reference to the psychoanalytic formulations of unconscious desire and transferential attachment, this article--with its focus on…

Heteromeles arbutifolia (Lindl.) M. Roemer: Christmasberry

Treesearch

Susan E. Meyer

2008-01-01

The genus Heteromeles has only a single species - H. arbutifolia (Lindl.) M. Roemer, also known as H. salicifolia (K. Presl.) Abrams (Phipps 1992). It is closely related to the large tropical genus Photinia Lindl., to which it has sometimes been referred. Christmasberry, also known as toyon, California holly, and hollywood, is a long-lived shrub or small tree, 2 to 10...

Analysis of the Microstructure of Titles in the INSPEC Data-Base

ERIC Educational Resources Information Center

And Others; Lynch, Michael F.

1973-01-01

A high degree of constancy has been found in the microstructure of titles of samples of the INSPEC data base taken over a three-year period. Character and digram frequencies are relatively stable, while variable-length character-strings characterizing samples separated by three years in time show close similarities. (2 references) (Author/SJ)

Partnerships in Teacher Education--Going beyond the Rhetoric, with Reference to the Norwegian Context

ERIC Educational Resources Information Center

Smith, Kari

2016-01-01

Teacher education plays a central role in education and relates to various stakeholders of education. Currently, teacher education is not perceived as the sole responsibility of higher education institutions, and they are expected to work closely together with other partners. In this paper, the concept of "partnership" is defined and…

Conceptual Analysis and Implications of Students' Individual Differences to Curriculum Implementation in Technical Education

ERIC Educational Resources Information Center

Akpan, Godwin A.; Essien, Emmanuel O.; Okure, Okure S.

2013-01-01

Individual differences refer to the unique ways each human being differs from another human being as expressed in behaviour or perceived in the physical appearance. Three factors of individual differences identified to be closely related to learning/acquisition of skills and performance of tasks. These are personality dimensions, self-efficacy and…

GN/C translation and rotation control parameters for AR/C (category 2)

NASA Technical Reports Server (NTRS)

Henderson, David M.

1991-01-01

Detailed analysis of the Automatic Rendezvous and Capture problem indicate a need for three different regions of mathematical description for the GN&C algorithms: (1) multi-vehicle orbital mechanics to the rendezvous interface point, i.e., within 100 n.; (2) relative motion solutions (such as Clohessy-Wiltshire type) from the far-field to the near-field interface, i.e., within 1 nm; and (3) close proximity motion, the nearfield motion where the relative differences in the gravitational and orbit inertial accelerations can be neglected from the equations of motion. This paper defines the reference coordinate frames and control parameters necessary to model the relative motion and attitude of spacecraft in the close proximity of another space system (Region 2 and 3) during the Automatic Rendezvous and Capture phase of an orbit operation.

A Comparative Study of Standardized Infinity Reference and Average Reference for EEG of Three Typical Brain States

PubMed Central

Zheng, Gaoxing; Qi, Xiaoying; Li, Yuzhu; Zhang, Wei; Yu, Yuguo

2018-01-01

The choice of different reference electrodes plays an important role in deciphering the functional meaning of electroencephalography (EEG) signals. In recent years, the infinity zero reference using the reference electrode standard technique (REST) has been increasingly applied, while the average reference (AR) was generally advocated as the best available reference option in previous classical EEG studies. Here, we designed EEG experiments and performed a direct comparison between the influences of REST and AR on EEG-revealed brain activity features for three typical brain behavior states (eyes-closed, eyes-open and music-listening). The analysis results revealed the following observations: (1) there is no significant difference in the alpha-wave-blocking effect during the eyes-open state compared with the eyes-closed state for both REST and AR references; (2) there was clear frontal EEG asymmetry during the resting state, and the degree of lateralization under REST was higher than that under AR; (3) the global brain functional connectivity density (FCD) and local FCD have higher values for REST than for AR under different behavior states; and (4) the value of the small-world network characteristic in the eyes-closed state is significantly (in full, alpha, beta and gamma frequency bands) higher than that in the eyes-open state, and the small-world effect under the REST reference is higher than that under AR. In addition, the music-listening state has a higher small-world network effect than the eyes-closed state. The above results suggest that typical EEG features might be more clearly presented by applying the REST reference than by applying AR when using a 64-channel recording. PMID:29593490

Taxonomic reassessment of Hydralmosaurus as Styxosaurus: new insights on the elasmosaurid neck evolution throughout the Cretaceous

PubMed Central

2016-01-01

Two extremely-long necked elasmosaurids, AMNH 1495, holotype of Hydralmosaurus serpentinus, and AMNH 5835, previously referred to H. serpentinus, are here reviewed in detail. Unique features of the cervical vertebrae, which are only present on elasmosaurids from the Western Interior Seaway, are recognized based on these specimens and by comparison with penecontemporaneous taxa with biogeographic affinities. Phylogenetic analysis, bivariate graphic analysis of cervical vertebrae proportions, comparisons of different cervical vertebral types, paleobiogeographic distribution and study of the elasmosaurid axial evolution throughout the Cretaceous are here integrated. As a result, at least two separate lineages within the Elasmosauridae are identified by independently acquired extremely-long necks (over 60 cervical vertebrae). First, a still scarcely known lineage is so far represented by the lower Cenomanian Thalassomedon haningtoni, the Turonian Libonectes morgani and close relatives. A second lineage is here defined as a new clade, the Styxosaurinae, which groups the Campanian genera Terminonatator, Styxosaurus (=‘Hydralmosaurus’), Albertonectes and Elasmosaurus, the two latter forming a derived branch that includes the most extreme amniote necks known to date (more than 70 cervical vertebrae). Phylogenetic analysis supports AMNH 1495 and AMNH 5835 as being closely related to Styxosaurus snowii. Therefore, the species Styxosaurus browni is re-validated, while AMNH 1495 is here referred to Styxosaurus sp. This research also recognizes the ‘Cimoliasauridae’ (nomen dubium) as a paraphyletic group but informative of a plesiomorphic cervical vertebral morphology of elasmosaurids which was persistent throughout the whole Cretaceous and from whom aristonectines, styxosaurines and Thalassomedon and close relatives are derived. The genus Hydralmosaurus is recommended for being abandoned. PMID:27019781

Taxonomic reassessment of Hydralmosaurus as Styxosaurus: new insights on the elasmosaurid neck evolution throughout the Cretaceous.

PubMed

Otero, Rodrigo A

2016-01-01

Two extremely-long necked elasmosaurids, AMNH 1495, holotype of Hydralmosaurus serpentinus, and AMNH 5835, previously referred to H. serpentinus, are here reviewed in detail. Unique features of the cervical vertebrae, which are only present on elasmosaurids from the Western Interior Seaway, are recognized based on these specimens and by comparison with penecontemporaneous taxa with biogeographic affinities. Phylogenetic analysis, bivariate graphic analysis of cervical vertebrae proportions, comparisons of different cervical vertebral types, paleobiogeographic distribution and study of the elasmosaurid axial evolution throughout the Cretaceous are here integrated. As a result, at least two separate lineages within the Elasmosauridae are identified by independently acquired extremely-long necks (over 60 cervical vertebrae). First, a still scarcely known lineage is so far represented by the lower Cenomanian Thalassomedon haningtoni, the Turonian Libonectes morgani and close relatives. A second lineage is here defined as a new clade, the Styxosaurinae, which groups the Campanian genera Terminonatator, Styxosaurus (='Hydralmosaurus'), Albertonectes and Elasmosaurus, the two latter forming a derived branch that includes the most extreme amniote necks known to date (more than 70 cervical vertebrae). Phylogenetic analysis supports AMNH 1495 and AMNH 5835 as being closely related to Styxosaurus snowii. Therefore, the species Styxosaurus browni is re-validated, while AMNH 1495 is here referred to Styxosaurus sp. This research also recognizes the 'Cimoliasauridae' (nomen dubium) as a paraphyletic group but informative of a plesiomorphic cervical vertebral morphology of elasmosaurids which was persistent throughout the whole Cretaceous and from whom aristonectines, styxosaurines and Thalassomedon and close relatives are derived. The genus Hydralmosaurus is recommended for being abandoned.

FOOT PLACEMENT IN A BODY REFERENCE FRAME DURING WALKING AND ITS RELATIONSHIP TO HEMIPARETIC WALKING PERFORMANCE

PubMed Central

Balasubramanian, Chitralakshmi K.; Neptune, Richard R.; Kautz, Steven A.

2010-01-01

Background Foot placement during walking is closely linked to the body position, yet it is typically quantified relative to the other foot. The purpose of this study was to quantify foot placement patterns relative to body post-stroke and investigate its relationship to hemiparetic walking performance. Methods Thirty-nine participants with hemiparesis walked on a split-belt treadmill at their self-selected speeds and twenty healthy participants walked at matched slow speeds. Anterior-posterior and medial-lateral foot placements (foot center-of-mass) relative to body (pelvis center-of-mass) quantified stepping in body reference frame. Walking performance was quantified using step length asymmetry ratio, percent of paretic propulsion and paretic weight support. Findings Participants with hemiparesis placed their paretic foot further anterior than posterior during walking compared to controls walking at matched slow speeds (p < .05). Participants also placed their paretic foot further lateral relative to pelvis than non-paretic (p < .05). Anterior-posterior asymmetry correlated with step length asymmetry and percent paretic propulsion but some persons revealed differing asymmetry patterns in the translating reference frame. Lateral foot placement asymmetry correlated with paretic weight support (r = .596; p < .001), whereas step widths showed no relation to paretic weight support. Interpretation Post-stroke gait is asymmetric when quantifying foot placement in a body reference frame and this asymmetry related to the hemiparetic walking performance and explained motor control mechanisms beyond those explained by step lengths and step widths alone. We suggest that biomechanical analyses quantifying stepping performance in impaired populations should investigate foot placement in a body reference frame. PMID:20193972

Foot placement in a body reference frame during walking and its relationship to hemiparetic walking performance.

PubMed

Balasubramanian, Chitralakshmi K; Neptune, Richard R; Kautz, Steven A

2010-06-01

Foot placement during walking is closely linked to the body position, yet it is typically quantified relative to the other foot. The purpose of this study was to quantify foot placement patterns relative to body post-stroke and investigate its relationship to hemiparetic walking performance. Thirty-nine participants with hemiparesis walked on a split-belt treadmill at their self-selected speeds and 20 healthy participants walked at matched slow speeds. Anterior-posterior and medial-lateral foot placements (foot center-of-mass) relative to body (pelvis center-of-mass) quantified stepping in body reference frame. Walking performance was quantified using step length asymmetry ratio, percent of paretic propulsion and paretic weight support. Participants with hemiparesis placed their paretic foot further anterior than posterior during walking compared to controls walking at matched slow speeds (P<.05). Participants also placed their paretic foot further lateral relative to pelvis than non-paretic (P<.05). Anterior-posterior asymmetry correlated with step length asymmetry and percent paretic propulsion but some persons revealed differing asymmetry patterns in the translating reference frame. Lateral foot placement asymmetry correlated with paretic weight support (r=.596; P<.001), whereas step widths showed no relation to paretic weight support. Post-stroke gait is asymmetric when quantifying foot placement in a body reference frame and this asymmetry related to the hemiparetic walking performance and explained motor control mechanisms beyond those explained by step lengths and step widths alone. We suggest that biomechanical analyses quantifying stepping performance in impaired populations should investigate foot placement in a body reference frame. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

[The viral hepatitis and its economic significance and prevention].

PubMed

Petruchuk, O E; Shchenin, V O; Bedrin, A V; Leshcheva, G A

2008-01-01

The reference of only part of diseases having contagious nature to the infectious pathology is closely related to the tradition of considering the infections as highly contagious diseases inclined to epidemic propagation. The data is presented related to the morbidity of viral hepatitis among the population of the City of Krasnodar. The corresponding economic losses and the achievements of medical preventive activities considering the social economic and other characteristics of the specific socium are considered.

MATLAB Toolboxes for Reference Electrode Standardization Technique (REST) of Scalp EEG

PubMed Central

Dong, Li; Li, Fali; Liu, Qiang; Wen, Xin; Lai, Yongxiu; Xu, Peng; Yao, Dezhong

2017-01-01

Reference electrode standardization technique (REST) has been increasingly acknowledged and applied as a re-reference technique to transform an actual multi-channels recordings to approximately zero reference ones in electroencephalography/event-related potentials (EEG/ERPs) community around the world in recent years. However, a more easy-to-use toolbox for re-referencing scalp EEG data to zero reference is still lacking. Here, we have therefore developed two open-source MATLAB toolboxes for REST of scalp EEG. One version of REST is closely integrated into EEGLAB, which is a popular MATLAB toolbox for processing the EEG data; and another is a batch version to make it more convenient and efficient for experienced users. Both of them are designed to provide an easy-to-use for novice researchers and flexibility for experienced researchers. All versions of the REST toolboxes can be freely downloaded at http://www.neuro.uestc.edu.cn/rest/Down.html, and the detailed information including publications, comments and documents on REST can also be found from this website. An example of usage is given with comparative results of REST and average reference. We hope these user-friendly REST toolboxes could make the relatively novel technique of REST easier to study, especially for applications in various EEG studies. PMID:29163006

MATLAB Toolboxes for Reference Electrode Standardization Technique (REST) of Scalp EEG.

PubMed

Dong, Li; Li, Fali; Liu, Qiang; Wen, Xin; Lai, Yongxiu; Xu, Peng; Yao, Dezhong

2017-01-01

Reference electrode standardization technique (REST) has been increasingly acknowledged and applied as a re-reference technique to transform an actual multi-channels recordings to approximately zero reference ones in electroencephalography/event-related potentials (EEG/ERPs) community around the world in recent years. However, a more easy-to-use toolbox for re-referencing scalp EEG data to zero reference is still lacking. Here, we have therefore developed two open-source MATLAB toolboxes for REST of scalp EEG. One version of REST is closely integrated into EEGLAB, which is a popular MATLAB toolbox for processing the EEG data; and another is a batch version to make it more convenient and efficient for experienced users. Both of them are designed to provide an easy-to-use for novice researchers and flexibility for experienced researchers. All versions of the REST toolboxes can be freely downloaded at http://www.neuro.uestc.edu.cn/rest/Down.html, and the detailed information including publications, comments and documents on REST can also be found from this website. An example of usage is given with comparative results of REST and average reference. We hope these user-friendly REST toolboxes could make the relatively novel technique of REST easier to study, especially for applications in various EEG studies.

The ecology of micro-organisms in a closed environment

NASA Technical Reports Server (NTRS)

Fox, L.

1971-01-01

Microorganisms under closed environmental ecological conditions with reference to astronauts infectious diseases, discussing bacteria growth in Biosatellite 2 and earth based closed chamber experiments

GEOS observation systems intercomparison investigation results

NASA Technical Reports Server (NTRS)

Berbert, J. H.

1974-01-01

The results of an investigation designed to determine the relative accuracy and precision of the different types of geodetic observation systems used by NASA is presented. A collocation technique was used to minimize the effects of uncertainties in the relative station locations and in the earth's gravity field model by installing accurate reference tracking systems close to the systems to be compared, and by precisely determining their relative survey. The Goddard laser and camera systems were shipped to selected sites, where they tracked the GEOS satellite simultaneously with other systems for an intercomparison observation.

Intellectual Freedom and Privacy: Comments on a National Program for Library and Information Services. Related Paper No. 10.

ERIC Educational Resources Information Center

Molz, R. Kathleen

The civil libertarian aspects of the National Program for Library and Information Science are analyzed. The five assumptions on which the program is based are closely examined for their references to the word "right." Details are given of the historic development of the library profession's increasing concern for the protection of…

The Development of Altruism with Special Reference to Human Relationships: A 10-Stage Theory.

PubMed

Ma, Hing Keung

2017-01-01

All human relationships involve some form of cost and benefit and altruism forms the foundation upon which human relationships are built. In this paper, a taxonomy of human relationships in terms of altruism was constructed. In the proposed taxonomy, human relationships are categorized into three major groups: primary group, secondary group, and tertiary group. The primary group consists of members that are very closely related to each other either by genetic relatedness (e.g., parents, siblings, and cousins) or social relatedness (e.g., mate and close friends) or both. The secondary group consists of members that are socially related but also less closely related with each other (e.g., people of the same political or religious group, teachers, mentors, acquaintances, neighbors, working colleagues, and strangers). Lastly, the tertiary group consists of members of other species. A 10-stage theory of altruism with special reference to human relationships is proposed. The affective, cognitive, and relationship aspects of each stage are delineated in details. There are two developmental principles of altruism. The first principle states that the development of altruism follows the 10-stage theory and moves from Stage 1: Egoism toward the higher stages of altruism slowly. The second developmental principle states that the taxonomy of human relationships is valid at any stage of altruism development. In other words, people at any stage of altruism are more altruistic toward their kin and mate, and then close friends, extended family members, and so on. They are least altruistic toward enemies and members of non-human species. In summary, the proposed developmental principle of altruism and human relationships is logical and robust. It is formulated based on the major developmental and social psychological theories. The theory has the potential in providing a useful framework for future studies on the development and evolution of human relationships.

The Development of Altruism with Special Reference to Human Relationships: A 10-Stage Theory

PubMed Central

Ma, Hing Keung

2017-01-01

All human relationships involve some form of cost and benefit and altruism forms the foundation upon which human relationships are built. In this paper, a taxonomy of human relationships in terms of altruism was constructed. In the proposed taxonomy, human relationships are categorized into three major groups: primary group, secondary group, and tertiary group. The primary group consists of members that are very closely related to each other either by genetic relatedness (e.g., parents, siblings, and cousins) or social relatedness (e.g., mate and close friends) or both. The secondary group consists of members that are socially related but also less closely related with each other (e.g., people of the same political or religious group, teachers, mentors, acquaintances, neighbors, working colleagues, and strangers). Lastly, the tertiary group consists of members of other species. A 10-stage theory of altruism with special reference to human relationships is proposed. The affective, cognitive, and relationship aspects of each stage are delineated in details. There are two developmental principles of altruism. The first principle states that the development of altruism follows the 10-stage theory and moves from Stage 1: Egoism toward the higher stages of altruism slowly. The second developmental principle states that the taxonomy of human relationships is valid at any stage of altruism development. In other words, people at any stage of altruism are more altruistic toward their kin and mate, and then close friends, extended family members, and so on. They are least altruistic toward enemies and members of non-human species. In summary, the proposed developmental principle of altruism and human relationships is logical and robust. It is formulated based on the major developmental and social psychological theories. The theory has the potential in providing a useful framework for future studies on the development and evolution of human relationships. PMID:29085818

Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

DOEpatents

Bavykin, Sergei G.; Mirzabekova, legal representative, Natalia V.; Mirzabekov, deceased, Andrei D.

2007-12-04

The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

Closed-loop control of renal perfusion pressure in physiological experiments.

PubMed

Campos-Delgado, D U; Bonilla, I; Rodríguez-Martínez, M; Sánchez-Briones, M E; Ruiz-Hernández, E

2013-07-01

This paper presents the design, experimental modeling, and control of a pump-driven renal perfusion pressure (RPP)-regulatory system to implement precise and relatively fast RPP regulation in rats. The mechatronic system is a simple, low-cost, and reliable device to automate the RPP regulation process based on flow-mediated occlusion. Hence, the regulated signal is the RPP measured in the left femoral artery of the rat, and the manipulated variable is the voltage applied to a dc motor that controls the occlusion of the aorta. The control system is implemented in a PC through the LabView software, and a data acquisition board NI USB-6210. A simple first-order linear system is proposed to approximate the dynamics in the experiment. The parameters of the model are chosen to minimize the error between the predicted and experimental output averaged from eight input/output datasets at different RPP operating conditions. A closed-loop servocontrol system based on a pole-placement PD controller plus dead-zone compensation was proposed for this purpose. First, the feedback structure was validated in simulation by considering parameter uncertainty, and constant and time-varying references. Several experimental tests were also conducted to validate in real time the closed-loop performance for stepwise and fast switching references, and the results show the effectiveness of the proposed automatic system to regulate the RPP in the rat, in a precise, accurate (mean error less than 2 mmHg) and relatively fast mode (10-15 s of response time).

Photobacterium damselae ssp. piscicida: detection by direct amplification of 16S rRNA gene sequences and genotypic variation as determined by amplified fragment length polymorphism (AFLP).

PubMed

Kvitt, H; Ucko, M; Colorni, A; Batargias, C; Zlotkin, A; Knibb, W

2002-04-05

A PCR protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16S rRNA gene sequences was developed. The procedure combines low annealing temperature that detects low titers of Photobacterium damselae but also related species, and high annealing temperature for the specific identification of P. damselae directly from infected fish. The PCR protocol was validated on 19 piscine isolates of P. damselae ssp. piscicida from different geographic regions (Japan, Italy, Spain, Greece and Israel), on spontaneously infected sea bream Sparus aurata and sea bass Dicentrarchus labrax, and on closely related American Type Culture Collection (ATCC) reference strains. PCR using high annealing temperature (64 degrees C) discriminated between P. damselae and closely related reference strains, including P. histaminum. Sixteen isolates of P. damselae ssp. piscicida, 2 P. damselae ssp. piscicida reference strains and 1 P. damselae ssp. damselae reference strain were subjected to Amplified Fragment Length Polymorphism (AFLP) analysis, and a similarity matrix was produced. Accordingly, the Japanese isolates of P. damselae ssp. piscicida were distinguished from the Mediterranean/European isolates at a cut-off value of 83% similarity. A further subclustering at a cut-off value of 97% allowed discrimination between the Israeli P. damselae ssp. piscicida isolates and the other Mediterranean/European isolates. The combination of PCR direct amplification and AFLP provides a 2-step procedure, where P. damselae is rapidly identified at genus level on the basis of its 16S rRNA gene sequence and then grouped into distinct clusters on the basis of AFLP polymorphisms. The first step of direct amplification is highly sensitive and has immediate practical consequences, offering fish farmers a rapid diagnosis, while the AFLP is more specific and detects intraspecific variation which, in our study, also reflected geographic correspondence. Because of its superior discriminative properties, AFLP can be an important tool for epidemiological and taxonomic studies of this highly homogeneous genus.

Designing EEG Neurofeedback Procedures to Enhance Open-Ended versus Closed-Ended Creative Potentials

ERIC Educational Resources Information Center

Lin, Wei-Lun; Shih, Yi-Ling

2016-01-01

Recent empirical evidence demonstrated that open-ended creativity (which refers to creativity measures that require various and numerous responses, such as divergent thinking) correlated with alpha brain wave activation, whereas closed-ended creativity (which refers to creativity measures that ask for one final correct answer, such as insight…

A Test and Reformulation of Reference Group and Role Correlates of Premarital Sexual Permissiveness Theory

ERIC Educational Resources Information Center

Libby, Roger W.; And Others

1978-01-01

Propositions concerned with reference group and role correlates of Ira Reiss' premarital sexual permissiveness theory were tested. Reiss' basic propositions are only partially supported. Closeness to mother's sexual standards is considerably more predictive of self-permissiveness than was obvious in Reiss' theory. Closeness to friends' and peers'…

Descriptive Drinking Norms: For Whom Does Reference Group Matter?*

PubMed Central

Larimer, Mary E.; Neighbors, Clayton; LaBrie, Joseph W.; Atkins, David C.; Lewis, Melissa A.; Lee, Christine M.; Kilmer, Jason R.; Kaysen, Debra L.; Pedersen, Eric r.; Montoya, Heidi; Hodge, Kimberley; Desai, Sruti; Hummer, Justin F.; Walter, Theresa

2011-01-01

Objective: Perceived descriptive drinking norms often differ from actual norms and are positively related to personal consumption. However, it is not clear how normative perceptions vary with specificity of the reference group. Are drinking norms more accurate and more closely related to drinking behavior as reference group specificity increases? Do these relationships vary as a function of participant demographics? The present study examined the relationship between perceived descriptive norms and drinking behavior by ethnicity (Asian or White), sex, and fraternity/sorority status. Method: Participants were 2,699 (58% female) White (75%) or Asian (25%) undergraduates from two universities who reported their own alcohol use and perceived descriptive norms for eight reference groups: "typical student"; same sex, ethnicity, or fraternity/sorority status; and all combinations of these three factors. Results: Participants generally reported the highest perceived norms for the most distal reference group (typical student), with perceptions becoming more accurate as individuals' similarity to the reference group increased. Despite increased accuracy, participants perceived that all reference groups drank more than was actually the case. Across specific subgroups (fraternity/sorority members and men) different patterns emerged. Fraternity/sorority members reliably reported higher estimates of drinking for reference groups that included fraternity/ sorority status, and, to a lesser extent, men reported higher estimates for reference groups that included men. Conclusions: The results suggest that interventions targeting normative misperceptions may need to provide feedback based on participant demography or group membership. Although reference group-specific feedback may be important for some subgroups, typical student feedback provides the largest normative discrepancy for the majority of students. PMID:21906510

An annotated bibliography of Pyrrone and BBB publications

NASA Technical Reports Server (NTRS)

Burks, H. D.

1972-01-01

This annotated bibliography covers the research and development of two closely related classes of high-temperature polymers, polyimidazopyrrolones (Pyrrone) and bisbenzimidazo-benzophenanthrolines (BBB), from their inception in 1965 through 1971. This compilation of available reference information is not inclusive, but it is sufficiently complete to aid the polymer chemist and materials engineer in the research and development of these two high temperature stable polymeric systems.

Tactical Unmanned Ground Vehicle Related Research References (BTA Study)

DTIC Science & Technology

1993-03-01

draw bar pull - 4,297 lbs; Engine - 65 hp air cooled diesel engine ; dual electrical motors, hydrostatic drive; Observation - three closed-circuit...8217 Munitions and Chemical Command. Commander, U. S. Army Chemical Research, Development, and Engineering Center. 40..... "Unmanned Air Vehicles Payloads...8217 Larry Brantley Advanced Systems Concepts Office Research, Development, and Engineering Center MARCH 1993 edetone qArs nal, Alabama 35898-5000

Co-Adaptive Aiding and Automation Enhance Operator Performance

DTIC Science & Technology

2013-03-01

activation system. There is a close relation between physiologically activated adaptive aiding and brain- computer interfaces ( BCI ). BCI here refers...classification of EEG signals (Farwell & Donchin, 1988). Physiologically activated adaptive aiding is, in a sense, a special case of BCI wherein the...as passive BCI , e.g. Zander, Kothe, Jatzev, & 3 Distribution A: Approved for public release; distribution unlimited. 88 ABW Cleared 05/13/2013

Comparing the biocidal properties of non-thermal plasma sources by reference protocol

NASA Astrophysics Data System (ADS)

Khun, Josef; Jirešová, Jana; Kujalová, Lucie; Hozák, Pavel; Scholtz, Vladimír

2017-10-01

The previously proposed reference protocol enabling easy comparison of biocidal properties of different non-thermal plasma sources has been followed and discussed. For inactivation tests the reference protocol has used spores of Gram positive bacterium Bacillus subtilis (ATCC 6633) deposited on a polycarbonate membrane as reference sample. In this work, biocidal properties of a negative glow corona, positive streamer corona, positive transient spark and cometary discharges are being compared in both open air and closed apparatus. Despite the total number of bacteria surviving 1 h exposure has decreased by up to 7 orders in closed apparatus, in open one, only weak inhibition bactericidal effect has been observed.

Collaborative derivation of reference intervals for major clinical laboratory tests in Japan.

PubMed

Ichihara, Kiyoshi; Yomamoto, Yoshikazu; Hotta, Taeko; Hosogaya, Shigemi; Miyachi, Hayato; Itoh, Yoshihisa; Ishibashi, Midori; Kang, Dongchon

2016-05-01

Three multicentre studies of reference intervals were conducted recently in Japan. The Committee on Common Reference Intervals of the Japan Society of Clinical Chemistry sought to establish common reference intervals for 40 laboratory tests which were measured in common in the three studies and regarded as well harmonized in Japan. The study protocols were comparable with recruitment mostly from hospital workers with body mass index ≤28 and no medications. Age and sex distributions were made equal to obtain a final data size of 6345 individuals. Between-subgroup differences were expressed as the SD ratio (between-subgroup SD divided by SD representing the reference interval). Between-study differences were all within acceptable levels, and thus the three datasets were merged. By adopting SD ratio ≥0.50 as a guide, sex-specific reference intervals were necessary for 12 assays. Age-specific reference intervals for females partitioned at age 45 were required for five analytes. The reference intervals derived by the parametric method resulted in appreciable narrowing of the ranges by applying the latent abnormal values exclusion method in 10 items which were closely associated with prevalent disorders among healthy individuals. Sex- and age-related profiles of reference values, derived from individuals with no abnormal results in major tests, showed peculiar patterns specific to each analyte. Common reference intervals for nationwide use were developed for 40 major tests, based on three multicentre studies by advanced statistical methods. Sex- and age-related profiles of reference values are of great relevance not only for interpreting test results, but for applying clinical decision limits specified in various clinical guidelines. © The Author(s) 2015.

Full-Authority Fault-Tolerant Electronic Engine Control Systems for Variable Cycle Engines.

DTIC Science & Technology

1981-12-01

Geometry or Fuel Flow Scheduled as a Function of Engine State, i.e. FIGV = f( N1 C2 ) Closed Loop - Geometry or Fuel Flow Modulated To Maintain an Engine...Low Pressure Turbine Inlet Area (A41) Closed Loop (Integral) N2, T22 Core Stream Exhaust Nozzle Area (AJE) Closed Loop (Integral) N1 , T2 Duct Stream...to remain at the breakpoint value while low rotor speed reference ( N1 reference) is scheduled to decrease as a function of power lever angle (PLA), to

Genetic relatedness of Legionella longbeachae isolates from human and environmental sources in Australia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lanser, J.A.; Doyle, R.; Sangster, N.

1990-09-01

The genetic relatedness of Legionella longbeachae isolated in Australia since 1987 was investigated by restriction fragment length polymorphism (RFLP) analysis and allozyme electrophoresis. Three radiolabeled probes were used in Southern hybridizations for the RFLP studies. They were Escherichia coli 16S and 23S rRNA and cloned fragments of L. longbeachae selected empirically from genomal banks in lambda and a cosmid. The legionellae included in the study comprised 11 Legionella longbeachae serogroup 1 organisms isolated form humans, 28 L. longbeachae serogroup 1 isolates from environmental sources, 3 L. longbeachae serogroup 2 environmental isolates. These were compared with the American Type Culture Collectionmore » reference strains of both serogroups and some other related Legionella species. Results of allozyme and RFLP analysis showed that all the isolates from humans and all but three of the environmental L. longbeachae serogroup 1 isolates were closely related. They were also closely related to L. longbeachae serogroup 1 ATCC 33462. There was wider variation among the three L. longbeachae serogroup 2 environmental isolates. One of these was closely related to L. longbeachae serogroup 2 ATCC 33484. RFLP studies with the rRNA probe provided the most discrimination among isolates but did not distinguish between the two serogroups.« less

Construction of Reference Chromosome-Scale Pseudomolecules for Potato: Integrating the Potato Genome with Genetic and Physical Maps

PubMed Central

Sharma, Sanjeev Kumar; Bolser, Daniel; de Boer, Jan; Sønderkær, Mads; Amoros, Walter; Carboni, Martin Federico; D’Ambrosio, Juan Martín; de la Cruz, German; Di Genova, Alex; Douches, David S.; Eguiluz, Maria; Guo, Xiao; Guzman, Frank; Hackett, Christine A.; Hamilton, John P.; Li, Guangcun; Li, Ying; Lozano, Roberto; Maass, Alejandro; Marshall, David; Martinez, Diana; McLean, Karen; Mejía, Nilo; Milne, Linda; Munive, Susan; Nagy, Istvan; Ponce, Olga; Ramirez, Manuel; Simon, Reinhard; Thomson, Susan J.; Torres, Yerisf; Waugh, Robbie; Zhang, Zhonghua; Huang, Sanwen; Visser, Richard G. F.; Bachem, Christian W. B.; Sagredo, Boris; Feingold, Sergio E.; Orjeda, Gisella; Veilleux, Richard E.; Bonierbale, Merideth; Jacobs, Jeanne M. E.; Milbourne, Dan; Martin, David Michael Alan; Bryan, Glenn J.

2013-01-01

The genome of potato, a major global food crop, was recently sequenced. The work presented here details the integration of the potato reference genome (DM) with a new sequence-tagged site marker−based linkage map and other physical and genetic maps of potato and the closely related species tomato. Primary anchoring of the DM genome assembly was accomplished by the use of a diploid segregating population, which was genotyped with several types of molecular genetic markers to construct a new ~936 cM linkage map comprising 2469 marker loci. In silico anchoring approaches used genetic and physical maps from the diploid potato genotype RH89-039-16 (RH) and tomato. This combined approach has allowed 951 superscaffolds to be ordered into pseudomolecules corresponding to the 12 potato chromosomes. These pseudomolecules represent 674 Mb (~93%) of the 723 Mb genome assembly and 37,482 (~96%) of the 39,031 predicted genes. The superscaffold order and orientation within the pseudomolecules are closely collinear with independently constructed high density linkage maps. Comparisons between marker distribution and physical location reveal regions of greater and lesser recombination, as well as regions exhibiting significant segregation distortion. The work presented here has led to a greatly improved ordering of the potato reference genome superscaffolds into chromosomal “pseudomolecules”. PMID:24062527

New Techniques in Computational Aerodynamics.

DTIC Science & Technology

1987-08-06

the nature of a continuous range of nearby flow fields is of fundmental significance in the design and performance of aircraft. To treat this...parameters. The resulting matrix of derivatives of flow quantities is referred to as the Jacobi matrix. The subsequent procedure is in principle now...employed here also results in a method which is closely related to the method of characteristics. Special account must be taken of the appearance of

German Views of Irregular Warfare

DTIC Science & Technology

2015-06-01

closely observed much of the Napoleonic Wars (1803–1815). His military experiences occurred during the end of the Age of Absolutism10 in Europe when...10 The Age of Absolutism refers to a period when absolute monarchs controlled much of Europe and is commonly considered to...concluded with a chapter related to irregular warfare and the nuclear age and an appendix dedicated to the training notes of the famed English

Two Androdioecious and One Dioecious New Species of Pristionchus (Nematoda: Diplogastridae): New Reference Points for the Evolution of Reproductive Mode

PubMed Central

Kanzaki, Natsumi; Ragsdale, Erik J.; Herrmann, Matthias; Susoy, Vladislav

2013-01-01

Rhabditid nematodes are one of a few animal taxa in which androdioecious reproduction, involving hermaphrodites and males, is found. In the genus Pristionchus, several cases of androdioecy are known, including the model species P. pacificus. A comprehensive understanding of the evolution of reproductive mode depends on dense taxon sampling and careful morphological and phylogenetic reconstruction. In this article, two new androdioecious species, P. boliviae n. sp. and P. mayeri n. sp., and one gonochoristic outgroup, P. atlanticus n. sp., are described on morphological, molecular, and biological evidence. Their phylogenetic relationships are inferred from 26 ribosomal protein genes and a partial SSU rRNA gene. Based on current representation, the new androdioecious species are sister taxa, indicating either speciation from an androdioecious ancestor or rapid convergent evolution in closely related species. Male sexual characters distinguish the new species, and new characters for six closely related Pristionchus species are presented. Male papillae are unusually variable in P. boliviae n. sp. and P. mayeri n. sp., consistent with the predictions of “selfing syndrome.” Description and phylogeny of new androdioecious species, supported by fuller outgroup representation, establish new reference points for mechanistic studies in the Pristionchus system by expanding its comparative context. PMID:24115783

Detection and characterization of Aichi virus 1 in pediatric patients with diarrhea in Thailand.

PubMed

Chuchaona, Watchaporn; Khamrin, Pattara; Yodmeeklin, Arpaporn; Kumthip, Kattareeya; Saikruang, Wilaiporn; Thongprachum, Aksara; Okitsu, Shoko; Ushijima, Hiroshi; Maneekarn, Niwat

2017-02-01

Kobuvirus is a newly discovered virus that belongs to the Kobuvirus genus in Picornaviridae family, which comprised of three species including Aichivirus A, Aichivirus B, and Aichivirus C. The kobuvirus isolated from human has been classified as Aichi virus 1 and belongs to Aichivirus A species. The present study aimed to assess the epidemiology and to perform molecular characterization of Aichi virus 1 in children admitted to hospitals with acute gastroenteritis in Chiang Mai, Thailand. A total of 923 fecal specimens collected from January, 2011 to December, 2013 were screened for the presence of Aichi virus 1 by RT semi-nested PCR. Out of 923 fecal specimens tested, Aichi virus 1 was detected with the prevalence of 2.6% (24/923). Of these, 0.3% (3/923) was genotype A and 2.3% (21/923) were genotype B. It is interesting to note that the genotype A showed the nucleotide sequence closely related to the Aichi virus reference strain isolated from sewage in Tunisia, while genotype B was most closely related to other human Aichi virus B reference strains. The results suggest that Aichi virus 1 of both genotypes A and B are circulating in pediatric patients in Thailand. J. Med. Virol. 89:234-238, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

The malleability of injunctive norms among college students.

PubMed

Prince, Mark A; Carey, Kate B

2010-11-01

Alcohol use among college students has been associated with injunctive norms, which refer to the perceived acceptability of excessive drinking, and descriptive norms, which refer to perceptions of actual drinking. The purpose of this study was to examine the effect of a brief injunctive norms manipulation on both injunctive and descriptive norms about drinking alcohol and to explore differences in the malleability of norms across referent groups, sex, and gender role. Participants were 265 undergraduates (43% male, 70% freshmen) who completed a web-based survey for course credit. A randomly selected half was exposed to a page of information-based feedback about typical student injunctive norms. Relative to the control condition, the manipulation produced lower injunctive and descriptive norms related to typical students' drinking but no change in either type of norm related to close friends. Femininity was associated with less permissive normative beliefs about the acceptability of excessive drinking whereas masculinity was associated with elevated perceptions of peer drinking, but neither sex nor gender role moderated the manipulation effect. We conclude that perceptions of peer approval of drinking are malleable with a very brief information-based manipulation. Copyright 2010 Elsevier Ltd. All rights reserved.

30 CFR 203.54 - How does my relief arrangement for an oil and gas lease operate if prices rise sharply?

Code of Federal Regulations, 2011 CFR

2011-07-01

... reference price, you must pay the effective royalty rate on all monthly production. (a) Your current reference price is a weighted average of daily closing prices on the NYMEX for light sweet crude oil and... average of daily closing prices on the NYMEX for light sweet crude oil and natural gas during the...

Rapid identification of Burkholderia mallei and Burkholderia pseudomallei by intact cell Matrix-assisted Laser Desorption/Ionisation mass spectrometric typing.

PubMed

Karger, Axel; Stock, Rüdiger; Ziller, Mario; Elschner, Mandy C; Bettin, Barbara; Melzer, Falk; Maier, Thomas; Kostrzewa, Markus; Scholz, Holger C; Neubauer, Heinrich; Tomaso, Herbert

2012-10-10

Burkholderia (B.) pseudomallei and B. mallei are genetically closely related species. B. pseudomallei causes melioidosis in humans and animals, whereas B. mallei is the causative agent of glanders in equines and rarely also in humans. Both agents have been classified by the CDC as priority category B biological agents. Rapid identification is crucial, because both agents are intrinsically resistant to many antibiotics. Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF MS) has the potential of rapid and reliable identification of pathogens, but is limited by the availability of a database containing validated reference spectra. The aim of this study was to evaluate the use of MALDI-TOF MS for the rapid and reliable identification and differentiation of B. pseudomallei and B. mallei and to build up a reliable reference database for both organisms. A collection of ten B. pseudomallei and seventeen B. mallei strains was used to generate a library of reference spectra. Samples of both species could be identified by MALDI-TOF MS, if a dedicated subset of the reference spectra library was used. In comparison with samples representing B. mallei, higher genetic diversity among B. pseudomallei was reflected in the higher average Eucledian distances between the mass spectra and a broader range of identification score values obtained with commercial software for the identification of microorganisms. The type strain of B. pseudomallei (ATCC 23343) was isolated decades ago and is outstanding in the spectrum-based dendrograms probably due to massive methylations as indicated by two intensive series of mass increments of 14 Da specifically and reproducibly found in the spectra of this strain. Handling of pathogens under BSL 3 conditions is dangerous and cumbersome but can be minimized by inactivation of bacteria with ethanol, subsequent protein extraction under BSL 1 conditions and MALDI-TOF MS analysis being faster than nucleic amplification methods. Our spectra demonstrated a higher homogeneity in B. mallei than in B. pseudomallei isolates. As expected for closely related species, the identification process with MALDI Biotyper software (Bruker Daltonik GmbH, Bremen, Germany) requires the careful selection of spectra from reference strains. When a dedicated reference set is used and spectra of high quality are acquired, it is possible to distinguish both species unambiguously. The need for a careful curation of reference spectra databases is stressed.

Rapid identification of Burkholderia mallei and Burkholderia pseudomallei by intact cell Matrix-assisted Laser Desorption/Ionisation mass spectrometric typing

PubMed Central

2012-01-01

Background Burkholderia (B.) pseudomallei and B. mallei are genetically closely related species. B. pseudomallei causes melioidosis in humans and animals, whereas B. mallei is the causative agent of glanders in equines and rarely also in humans. Both agents have been classified by the CDC as priority category B biological agents. Rapid identification is crucial, because both agents are intrinsically resistant to many antibiotics. Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF MS) has the potential of rapid and reliable identification of pathogens, but is limited by the availability of a database containing validated reference spectra. The aim of this study was to evaluate the use of MALDI-TOF MS for the rapid and reliable identification and differentiation of B. pseudomallei and B. mallei and to build up a reliable reference database for both organisms. Results A collection of ten B. pseudomallei and seventeen B. mallei strains was used to generate a library of reference spectra. Samples of both species could be identified by MALDI-TOF MS, if a dedicated subset of the reference spectra library was used. In comparison with samples representing B. mallei, higher genetic diversity among B. pseudomallei was reflected in the higher average Eucledian distances between the mass spectra and a broader range of identification score values obtained with commercial software for the identification of microorganisms. The type strain of B. pseudomallei (ATCC 23343) was isolated decades ago and is outstanding in the spectrum-based dendrograms probably due to massive methylations as indicated by two intensive series of mass increments of 14 Da specifically and reproducibly found in the spectra of this strain. Conclusions Handling of pathogens under BSL 3 conditions is dangerous and cumbersome but can be minimized by inactivation of bacteria with ethanol, subsequent protein extraction under BSL 1 conditions and MALDI-TOF MS analysis being faster than nucleic amplification methods. Our spectra demonstrated a higher homogeneity in B. mallei than in B. pseudomallei isolates. As expected for closely related species, the identification process with MALDI Biotyper software (Bruker Daltonik GmbH, Bremen, Germany) requires the careful selection of spectra from reference strains. When a dedicated reference set is used and spectra of high quality are acquired, it is possible to distinguish both species unambiguously. The need for a careful curation of reference spectra databases is stressed. PMID:23046611

Parametric analysis of closed cycle magnetohydrodynamic (MHD) power plants

NASA Technical Reports Server (NTRS)

Owens, W.; Berg, R.; Murthy, R.; Patten, J.

1981-01-01

A parametric analysis of closed cycle MHD power plants was performed which studied the technical feasibility, associated capital cost, and cost of electricity for the direct combustion of coal or coal derived fuel. Three reference plants, differing primarily in the method of coal conversion utilized, were defined. Reference Plant 1 used direct coal fired combustion while Reference Plants 2 and 3 employed on site integrated gasifiers. Reference Plant 2 used a pressurized gasifier while Reference Plant 3 used a ""state of the art' atmospheric gasifier. Thirty plant configurations were considered by using parametric variations from the Reference Plants. Parametric variations include the type of coal (Montana Rosebud or Illinois No. 6), clean up systems (hot or cold gas clean up), on or two stage atmospheric or pressurized direct fired coal combustors, and six different gasifier systems. Plant sizes ranged from 100 to 1000 MWe. Overall plant performance was calculated using two methodologies. In one task, the channel performance was assumed and the MHD topping cycle efficiencies were based on the assumed values. A second task involved rigorous calculations of channel performance (enthalpy extraction, isentropic efficiency and generator output) that verified the original (task one) assumptions. Closed cycle MHD capital costs were estimated for the task one plants; task two cost estimates were made for the channel and magnet only.

A preliminary verification of the floating reference measurement method for non-invasive blood glucose sensing

NASA Astrophysics Data System (ADS)

Min, Xiaolin; Liu, Rong; Fu, Bo; Xu, Kexin

2017-06-01

In the non-invasive sensing of blood glucose by near-infrared diffuse reflectance spectroscopy, the spectrum is highly susceptible to the unstable and complicated background variations from the human body and the environment. In in vitro analyses, background variations are usually corrected by the spectrum of a standard reference sample that has similar optical properties to the analyte of interest. However, it is hard to find a standard sample for the in vivo measurement. Therefore, the floating reference measurement method is proposed to enable relative measurements in vivo, where the spectra under some special source-detector distance, defined as the floating reference position, are insensitive to the changes in glucose concentration due to the absorption effect and scattering effect. Because the diffuse reflectance signals at the floating reference positions only reflect the information on background variations during the measurement, they can be used as the internal reference. In this paper, the theoretical basis of the floating reference positions in a semi-infinite turbid medium was discussed based on the steady-state diffusion equation and its analytical solutions in a semi-infinite turbid medium (under the extrapolated boundary conditions). Then, Monte-Carlo (MC) simulations and in vitro experiments based on a custom-built continuous-moving spatially resolving double-fiber NIR measurement system, configured with two types of light source, a super luminescent diode (SLD) and a super-continuum laser, were carried out to verify the existence of the floating reference position in 5%, 10% and 20% Intralipid solutions. The results showed that the simulation values of the floating reference positions are close to the theoretical results, with a maximum deviation of approximately 0.3 mm in 1100-1320 nm. Great differences can be observed in 1340-1400 nm because the optical properties of Intralipid in this region don not satisfy the conditions of the steady-state diffusion equation. For the in vitro experiments, floating reference positions exist in 1220 nm and 1320 nm under two types of light source, and the results are quite close. However, the reference positions obtained from experiments are further from the light source compared with those obtained in the MC simulation. For the turbid media and the wavelengths investigated, the difference is up to 1 mm. This study is important for the design of optical fibers to be applied in the floating reference measurement.

Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

DOEpatents

Bavykin, Sergei G.; Mirzabekov, Andrei D.

2007-10-30

The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

The influence of plasma flows bringing the magnetotail back to a more symmetric configuration

NASA Astrophysics Data System (ADS)

Reistad, J. P.; Østgaard, N.; Laundal, K.; Tenfjord, P.; Snekvik, K.; Haaland, S.; Milan, S. E.; Ohma, A.; Grocott, A.; Oksavik, K.

2017-12-01

Research from the past decades, most importantly conjugate studies, have shown extensive evidence of the Earth's closed magnetotail being highly displaced from the quiet-day configuration in response to the IMF interacting with the magnetosphere. By displaced we here refer to the mapping of magnetic field-lines from one hemisphere to the other. The large-scale ionospheric convection related to such displaced closed field-lines has also been studied, showing that the footprint in one hemisphere tend to move faster to reduce the displacement, a process we refer to as the restoring of symmetry. Although the appearance and occurrence of the plasma flows related to the restoring of symmetry has been shown to have a strong Interplanetary Magnetic Field (IMF) control, its dynamics and relation to internal magnetospheric processes are unknown. Using multiple years of line-of-sight measurements of the ionospheric plasma convection from the Super Dual Auroral Radar Network binned by IMF, season, and SML index, we have found that the restoring symmetry flows dominate the average convection pattern in the nightside ionosphere during low levels of magnetotail activity, quantified by the SML index. For increasing magnetotail activity, signatures of the restoring symmetry process become less and less pronounced in the global average convection maps. This effect is seen for all clock angles away from IMF By = 0. These results are relevant in order to better understand the dynamic evolution of flux-tubes in the asymmetric magnetosphere.

Genomic resources for gene discovery, functional genome annotation, and evolutionary studies of maize and its close relatives.

PubMed

Wang, Chao; Shi, Xue; Liu, Lin; Li, Haiyan; Ammiraju, Jetty S S; Kudrna, David A; Xiong, Wentao; Wang, Hao; Dai, Zhaozhao; Zheng, Yonglian; Lai, Jinsheng; Jin, Weiwei; Messing, Joachim; Bennetzen, Jeffrey L; Wing, Rod A; Luo, Meizhong

2013-11-01

Maize is one of the most important food crops and a key model for genetics and developmental biology. A genetically anchored and high-quality draft genome sequence of maize inbred B73 has been obtained to serve as a reference sequence. To facilitate evolutionary studies in maize and its close relatives, much like the Oryza Map Alignment Project (OMAP) (www.OMAP.org) bacterial artificial chromosome (BAC) resource did for the rice community, we constructed BAC libraries for maize inbred lines Zheng58, Chang7-2, and Mo17 and maize wild relatives Zea mays ssp. parviglumis and Tripsacum dactyloides. Furthermore, to extend functional genomic studies to maize and sorghum, we also constructed binary BAC (BIBAC) libraries for the maize inbred B73 and the sorghum landrace Nengsi-1. The BAC/BIBAC vectors facilitate transfer of large intact DNA inserts from BAC clones to the BIBAC vector and functional complementation of large DNA fragments. These seven Zea Map Alignment Project (ZMAP) BAC/BIBAC libraries have average insert sizes ranging from 92 to 148 kb, organellar DNA from 0.17 to 2.3%, empty vector rates between 0.35 and 5.56%, and genome equivalents of 4.7- to 8.4-fold. The usefulness of the Parviglumis and Tripsacum BAC libraries was demonstrated by mapping clones to the reference genome. Novel genes and alleles present in these ZMAP libraries can now be used for functional complementation studies and positional or homology-based cloning of genes for translational genomics.

Integrated Model-Based Controls and PHM for Improving Turbine Engine Performance, Reliability, and Cost

DTIC Science & Technology

2009-09-01

capable of surviving the high-temperature, high- vibration environment of a jet engine. Active control spans active surge/stall control and three...other closely related areas, viz., active combustion control (references 21-22), active noise control, and active vibration control. All of these are...self-powered sensors that harvest energy from engine heat or vibrations replace sensors that require power. The long-term vision is one of a

The "Martes complex"-- an opportunity to bring together marten, fisher, sable, wolverine, and tayra biologists

Treesearch

Gilbert Proulx; Keith B. Aubry

2014-01-01

Recent phylogenetic studies have shown that the genus Martes is polyphyletic with respect to the Fisher (Pekania pennanti), which is more closely related to the Wolverine (Gulo gulo) and Tayra (Eira barbara) than it is to the martens. We refer to the 11 species in the genera Martes, Pekania, Gulo, and Eira as the “Martes Complex”. Because the species comprising the...

A crossbred reference population can improve the response to genomic selection for crossbred performance.

PubMed

Esfandyari, Hadi; Sørensen, Anders Christian; Bijma, Piter

2015-09-29

Breeding goals in a crossbreeding system should be defined at the commercial crossbred level. However, selection is often performed to improve purebred performance. A genomic selection (GS) model that includes dominance effects can be used to select purebreds for crossbred performance. Optimization of the GS model raises the question of whether marker effects should be estimated from data on the pure lines or crossbreds. Therefore, the first objective of this study was to compare response to selection of crossbreds by simulating a two-way crossbreeding program with either a purebred or a crossbred training population. We assumed a trait of interest that was controlled by loci with additive and dominance effects. Animals were selected on estimated breeding values for crossbred performance. There was no genotype by environment interaction. Linkage phase and strength of linkage disequilibrium between quantitative trait loci (QTL) and single nucleotide polymorphisms (SNPs) can differ between breeds, which causes apparent effects of SNPs to be line-dependent. Thus, our second objective was to compare response to GS based on crossbred phenotypes when the line origin of alleles was taken into account or not in the estimation of breeding values. Training on crossbred animals yielded a larger response to selection in crossbred offspring compared to training on both pure lines separately or on both pure lines combined into a single reference population. Response to selection in crossbreds was larger if both phenotypes and genotypes were collected on crossbreds than if phenotypes were only recorded on crossbreds and genotypes on their parents. If both parental lines were distantly related, tracing the line origin of alleles improved genomic prediction, whereas if both parental lines were closely related and the reference population was small, it was better to ignore the line origin of alleles. Response to selection in crossbreeding programs can be increased by training on crossbred genotypes and phenotypes. Moreover, if the reference population is sufficiently large and both pure lines are not very closely related, tracing the line origin of alleles in crossbreds improves genomic prediction.

Impact of the choice of reference genome on the ability of the core genome SNV methodology to distinguish strains of Salmonella enterica serovar Heidelberg.

PubMed

Usongo, Valentine; Berry, Chrystal; Yousfi, Khadidja; Doualla-Bell, Florence; Labbé, Genevieve; Johnson, Roger; Fournier, Eric; Nadon, Celine; Goodridge, Lawrence; Bekal, Sadjia

2018-01-01

Salmonella enterica serovar Heidelberg (S. Heidelberg) is one of the top serovars causing human salmonellosis. The core genome single nucleotide variant pipeline (cgSNV) is one of several whole genome based sequence typing methods used for the laboratory investigation of foodborne pathogens. SNV detection using this method requires a reference genome. The purpose of this study was to investigate the impact of the choice of the reference genome on the cgSNV-informed phylogenetic clustering and inferred isolate relationships. We found that using a draft or closed genome of S. Heidelberg as reference did not impact the ability of the cgSNV methodology to differentiate among 145 S. Heidelberg isolates involved in foodborne outbreaks. We also found that using a distantly related genome such as S. Dublin as choice of reference led to a loss in resolution since some sporadic isolates were found to cluster together with outbreak isolates. In addition, the genetic distances between outbreak isolates as well as between outbreak and sporadic isolates were overall reduced when S. Dublin was used as the reference genome as opposed to S. Heidelberg.

How do reference montage and electrodes setup affect the measured scalp EEG potentials?

NASA Astrophysics Data System (ADS)

Hu, Shiang; Lai, Yongxiu; Valdes-Sosa, Pedro A.; Bringas-Vega, Maria L.; Yao, Dezhong

2018-04-01

Objective. Human scalp electroencephalogram (EEG) is widely applied in cognitive neuroscience and clinical studies due to its non-invasiveness and ultra-high time resolution. However, the representativeness of the measured EEG potentials for the underneath neural activities is still a problem under debate. This study aims to investigate systematically how both reference montage and electrodes setup affect the accuracy of EEG potentials. Approach. First, the standard EEG potentials are generated by the forward calculation with a single dipole in the neural source space, for eleven channel numbers (10, 16, 21, 32, 64, 85, 96, 128, 129, 257, 335). Here, the reference is the ideal infinity implicitly determined by forward theory. Then, the standard EEG potentials are transformed to recordings with different references including five mono-polar references (Left earlobe, Fz, Pz, Oz, Cz), and three re-references (linked mastoids (LM), average reference (AR) and reference electrode standardization technique (REST)). Finally, the relative errors between the standard EEG potentials and the transformed ones are evaluated in terms of channel number, scalp regions, electrodes layout, dipole source position and orientation, as well as sensor noise and head model. Main results. Mono-polar reference recordings are usually of large distortions; thus, a re-reference after online mono-polar recording should be adopted in general to mitigate this effect. Among the three re-references, REST is generally superior to AR for all factors compared, and LM performs worst. REST is insensitive to head model perturbation. AR is subject to electrodes coverage and dipole orientation but no close relation with channel number. Significance. These results indicate that REST would be the first choice of re-reference and AR may be an alternative option for high level sensor noise case. Our findings may provide the helpful suggestions on how to obtain the EEG potentials as accurately as possible for cognitive neuroscientists and clinicians.

Aluminium-gold reference material for the k0-standardisation of neutron activation analysis

NASA Astrophysics Data System (ADS)

Ingelbrecht, C.; Peetermans, F.; De Corte, F.; De Wispelaere, A.; Vandecasteele, C.; Courtijn, E.; D'Hondt, P.

1991-05-01

Gold is an excellent comparator material for the k0-standardisation of neutron activation analysis because of its convenient and well defined nuclear properties. The most suitable form for a reference material is a dilute aluminium-gold alloy, for which the self-shielding effect for neutrons is small. Castings of composition Al-0.1 wt.% Au were prepared by crucible-less levitation melting, which gives close control of ingot composition with minimal contamination of the melt. The alloy composition was checked using induction-coupled plasma source emission spectrometry. The homogeneity of the alloy was measured by neutron activation analysis and a relative standard deviation of the gold content of 0.30% was found (10 mg samples). Metallography revealed a homogeneous distribution of AuAl 2 particles. The alloy was certified as Reference Material CBNM-530, with certified gold mass fraction 0.100±0.002 wt.%.

Increased Biomass Production by Mesophilic Food-Associated Bacteria through Lowering the Growth Temperature from 30°C to 10°C.

PubMed

Seel, Waldemar; Derichs, Julia; Lipski, André

2016-07-01

Five isolates from chilled food and refrigerator inner surfaces and closely related reference strains of the species Escherichia coli, Listeria monocytogenes, Staphylococcus xylosus, Bacillus cereus, Pedobacter nutrimenti, and Pedobacter panaciterrae were tested for the effect of growth temperature (30°C and 10°C) on biomass formation. Growth was monitored via optical density, and biomass formation was measured at the early stationary phase based on the following parameters in complex and defined media: viable cell count, total cell count, cell dry weight, whole-cell protein content, and cell morphology. According to the lack of growth at 1°C, all strains were assigned to the thermal class of mesophiles. Glucose and ammonium consumption related to cell yield were analyzed in defined media. Except for the protein content, temperature had a significant (t test, P < 0.05) effect on all biomass formation parameters for each strain. The results show a significant difference between the isolates and the related reference strains. Isolates achieved an increase in biomass production between 20% and 110% at the 10°C temperature, which is 15 to 25°C lower than their maximum growth rate temperatures. In contrast, reference strains showed a maximum increase of only about 25%, and some reference strains showed no increase or a decrease of approximately 25%. As expected, growth rates for all strains were higher at 30°C than at 10°C, while biomass production for isolates was higher at 10°C than at 30°C. In contrast, the reference strains showed similar growth yields at the two temperatures. This also demonstrates for mesophilic bacterial strains more efficient nutrient assimilation during growth at low temperatures. Until now, this characteristic was attributed only to psychrophilic microorganisms. For several psychrophilic species, increased biomass formation was described at temperatures lower than optimum growth temperatures, which are defined by the highest growth rate. This work shows increased biomass formation at low growth temperatures for mesophilic isolates. A comparison with closely related reference strains from culture collections showed a significantly smaller increase or no increase in biomass formation. This indicates a loss of specific adaptive mechanisms (e.g., cold adaptation) for mesophiles during long-term cultivation. The increased biomass production for mesophiles under low-temperature conditions opens new avenues for a more efficient biotechnological transformation of nutrients to microbial biomass. These findings may also be important for risk assessment of cooled foods since risk potential is often correlated with the cell numbers present in food samples. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

47 CFR 79.100 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 4 2013-10-01 2013-10-01 false Incorporation by reference. 79.100 Section 79.100 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES CLOSED CAPTIONING AND VIDEO DESCRIPTION OF VIDEO PROGRAMMING § 79.100 Incorporation by reference. (a) The materials...

47 CFR 79.100 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 4 2012-10-01 2012-10-01 false Incorporation by reference. 79.100 Section 79.100 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES CLOSED CAPTIONING AND VIDEO DESCRIPTION OF VIDEO PROGRAMMING § 79.100 Incorporation by reference. (a) The materials...

Disturbance rejection performance analyses of closed loop control systems by reference to disturbance ratio.

PubMed

Alagoz, Baris Baykant; Deniz, Furkan Nur; Keles, Cemal; Tan, Nusret

2015-03-01

This study investigates disturbance rejection capacity of closed loop control systems by means of reference to disturbance ratio (RDR). The RDR analysis calculates the ratio of reference signal energy to disturbance signal energy at the system output and provides a quantitative evaluation of disturbance rejection performance of control systems on the bases of communication channel limitations. Essentially, RDR provides a straightforward analytical method for the comparison and improvement of implicit disturbance rejection capacity of closed loop control systems. Theoretical analyses demonstrate us that RDR of the negative feedback closed loop control systems are determined by energy spectral density of controller transfer function. In this manner, authors derived design criteria for specifications of disturbance rejection performances of PID and fractional order PID (FOPID) controller structures. RDR spectra are calculated for investigation of frequency dependence of disturbance rejection capacity and spectral RDR analyses are carried out for PID and FOPID controllers. For the validation of theoretical results, simulation examples are presented. Copyright © 2014 ISA. Published by Elsevier Ltd. All rights reserved.

The relation between body semantics and spatial body representations.

PubMed

van Elk, Michiel; Blanke, Olaf

2011-11-01

The present study addressed the relation between body semantics (i.e. semantic knowledge about the human body) and spatial body representations, by presenting participants with word pairs, one below the other, referring to body parts. The spatial position of the word pairs could be congruent (e.g. EYE / MOUTH) or incongruent (MOUTH / EYE) with respect to the spatial position of the words' referents. In addition, the spatial distance between the words' referents was varied, resulting in word pairs referring to body parts that are close (e.g. EYE / MOUTH) or far in space (e.g. EYE / FOOT). A spatial congruency effect was observed when subjects made an iconicity judgment (Experiments 2 and 3) but not when making a semantic relatedness judgment (Experiment 1). In addition, when making a semantic relatedness judgment (Experiment 1) reaction times increased with increased distance between the body parts but when making an iconicity judgment (Experiments 2 and 3) reaction times decreased with increased distance. These findings suggest that the processing of body-semantics results in the activation of a detailed visuo-spatial body representation that is modulated by the specific task requirements. We discuss these new data with respect to theories of embodied cognition and body semantics. Copyright © 2011 Elsevier B.V. All rights reserved.

Complete genome sequence analysis of novel human bocavirus reveals genetic recombination between human bocavirus 2 and human bocavirus 4.

PubMed

Khamrin, Pattara; Okitsu, Shoko; Ushijima, Hiroshi; Maneekarn, Niwat

2013-07-01

Epidemiological surveillance of human bocavirus (HBoV) was conducted on fecal specimens collected from hospitalized children with diarrhea in Chiang Mai, Thailand in 2011. By partial sequence analysis of VP1 gene, an unusual strain of HBoV (CMH-S011-11), was initially identified as HBoV4. The complete genome sequence of CMH-S011-11 was performed and analyzed further to clarify whether it was a recombinant strain or a new HBoV variant. Analysis of complete genome sequence revealed that the coding sequence starting from NS1, NP1 to VP1/VP2 was 4795 nucleotides long. Interestingly, the nucleotide sequence of NS1 gene of CMH-S011-11 was most closely related to the HBoV2 reference strains detected in Pakistan, which contradicted to the initial genotyping result of the partial VP1 region in the previous study. In addition, comparison of NP1 nucleotide sequence of CMH-S011-11 with those of other HBoV1-4 reference strains also revealed a high level of sequence identity with HBoV2. On the other hand, nucleotide sequence of VP1/VP2 gene of CMH-S011-11 was most closely related to those of HBoV4 reference strains detected in Nigeria. The overall full-length sequence analysis revealed that this CMH-S011-11 was grouped within HBoV4 species, but located in a separate branch from other HBoV4 prototype strains. Recombination analysis revealed that CMH-S011-11 was the result of recombination between HBoV2 and HBoV4 strains with the break point located near the start codon of VP2. Copyright © 2013 Elsevier B.V. All rights reserved.

The Tuareg: A Nation Without Borders? A CNA Strategic Studies Conference Report

DTIC Science & Technology

2013-05-01

claim to “ethnic specificity.” An ethnic branch of the Berbers , the Tuareg are culturally and linguistically unique. Despite strong tribal and caste...The Tuareg are an ethnic branch of the Berbers but also have mem- bers of Arab and black African ancestry. Often referred to as the “Kurds of the...cluster of closely related dia- lects of a southern Berber language group (Tamasheq, Tamajeq, Tamahaq), which, while different, are mutually

Interagency Review of Prior Inspector General Recommendations Related to U.S. Export Controls. Volume I

DTIC Science & Technology

2008-09-01

applications that require referral to the Office of Export Enforcement ( OEE ), and sends them simultaneously to OEE and licensing officers in Export...Enforcement ( OEE ) and the Office of Enforcement Analysis (OEA). We also spoke with staff from OEE ’ s Boston field office regarding closed...OExS staff should refer them to the Office of Export Enforcement ( OEE ). However, in the two cases of noncompliance, both exporters had shipped

Smart Healthcare Agility Management - Tojisha Doctrine by Self-synchronization

DTIC Science & Technology

2014-06-01

impossibility at our age. One is the technology of space elevator and another is the space-walk tourism which the project has existed since the mid-20 th...In regards to the technologies of space-walk tourism , increase of patents related to the technologies of Pulse Detention Engine worldwide is some...medicine, and there is some reference cases such as "open agility" and "closed agility" is immersed in training specifically in sports science which

Estimation of the displacements among distant events based on parallel tracking of events in seismic traces under uncertainty

NASA Astrophysics Data System (ADS)

Huamán Bustamante, Samuel G.; Cavalcanti Pacheco, Marco A.; Lazo Lazo, Juan G.

2018-07-01

The method we propose in this paper seeks to estimate interface displacements among strata related with reflection seismic events, in comparison to the interfaces at other reference points. To do so, we search for reflection events in the reference point of a second seismic trace taken from the same 3D survey and close to a well. However, the nature of the seismic data introduces uncertainty in the results. Therefore, we perform an uncertainty analysis using the standard deviation results from several experiments with cross-correlation of signals. To estimate the displacements of events in depth between two seismic traces, we create a synthetic seismic trace with an empirical wavelet and the sonic log of the well, close to the second seismic trace. Then, we relate the events of the seismic traces to the depth of the sonic log. Finally, we test the method with data from the Namorado Field in Brazil. The results show that the accuracy of the event estimated depth depends on the results of parallel cross-correlation, primarily those from the procedures used in the integration of seismic data with data from the well. The proposed approach can correctly identify several similar events in two seismic traces without requiring all seismic traces between two distant points of interest to correlate strata in the subsurface.

Biogeography of a Novel Ensifer meliloti Clade Associated with the Australian Legume Trigonella suavissima

PubMed Central

Elia, Patrick; Brockwell, John; Golemboski, Daniel; van Berkum, Peter

2017-01-01

ABSTRACT Here, we describe a novel clade within Ensifer meliloti and consider how geographic and ecological isolation contributed to the limited distribution of this group. Members of the genus Ensifer are best known for their ability to form nitrogen-fixing symbioses with forage legumes of three related genera, Medicago L., Melilotus Mill., and Trigonella L., which are members of the tribe Trifolieae. These legumes have a natural distribution extending from the Mediterranean Basin through western Asia, where there is an unsurpassed number of species belonging to these genera. Trigonella suavissima L. is unusual in that it is the only species in the tribe Trifolieae that is native to Australia. We compared the genetic diversity and taxonomic placement of rhizobia nodulating T. suavissima with those of members of an Ensifer reference collection. Our goal was to determine if the T. suavissima rhizobial strains, like their plant host, are naturally limited to the Australian continent. We used multilocus sequence analysis to estimate the genetic relatedness of 56 T. suavissima symbionts to 28 Ensifer reference strains. Sequence data were partitioned according to the replicons in which the loci are located. The results were used to construct replicon-specific phylogenetic trees. In both the chromosomal and chromid trees, the Australian strains formed a distinct clade within E. meliloti. The strains also shared few alleles with Ensifer reference strains from other continents. Carbon source utilization assays revealed that the strains are also unusual in their ability to utilize 2-oxoglutarate as a sole carbon source. A strategy was outlined for locating similar strains elsewhere. IMPORTANCE In this study, we employed a biogeographical approach to investigate the origins of a symbiotic relationship between an Australian legume and its nitrogen-fixing rhizobia. The question of the ancestral origins of these symbionts is based on the observation that the legume host is not closely related to other native Australian legumes. Previous research has shown that the legume host Trigonella suavissima is instead closely related to legumes native to the Mediterranean Basin and western Asia, suggesting that it may have been introduced in Australia from those regions. This led to the question of whether its rhizobia may have been introduced as well. In this study, we were unable to find persuasive evidence supporting this hypothesis. Instead, our results suggest either that the T. suavissima rhizobia are native to Australia or that our methods for locating their close relatives elsewhere are inadequate. A strategy to investigate the latter alternative is proposed. PMID:28283520

Transcriptome-based differentiation of closely-related Miscanthus lines.

PubMed

Chouvarine, Philippe; Cooksey, Amanda M; McCarthy, Fiona M; Ray, David A; Baldwin, Brian S; Burgess, Shane C; Peterson, Daniel G

2012-01-01

Distinguishing between individuals is critical to those conducting animal/plant breeding, food safety/quality research, diagnostic and clinical testing, and evolutionary biology studies. Classical genetic identification studies are based on marker polymorphisms, but polymorphism-based techniques are time and labor intensive and often cannot distinguish between closely related individuals. Illumina sequencing technologies provide the detailed sequence data required for rapid and efficient differentiation of related species, lines/cultivars, and individuals in a cost-effective manner. Here we describe the use of Illumina high-throughput exome sequencing, coupled with SNP mapping, as a rapid means of distinguishing between related cultivars of the lignocellulosic bioenergy crop giant miscanthus (Miscanthus × giganteus). We provide the first exome sequence database for Miscanthus species complete with Gene Ontology (GO) functional annotations. A SNP comparative analysis of rhizome-derived cDNA sequences was successfully utilized to distinguish three Miscanthus × giganteus cultivars from each other and from other Miscanthus species. Moreover, the resulting phylogenetic tree generated from SNP frequency data parallels the known breeding history of the plants examined. Some of the giant miscanthus plants exhibit considerable sequence divergence. Here we describe an analysis of Miscanthus in which high-throughput exome sequencing was utilized to differentiate between closely related genotypes despite the current lack of a reference genome sequence. We functionally annotated the exome sequences and provide resources to support Miscanthus systems biology. In addition, we demonstrate the use of the commercial high-performance cloud computing to do computational GO annotation.

Is an eclipse described in the Odyssey?

PubMed

Baikouzis, Constantino; Magnasco, Marcelo O

2008-07-01

Plutarch and Heraclitus believed a certain passage in the 20th book of the Odyssey ("Theoclymenus's prophecy") to be a poetic description of a total solar eclipse. In the late 1920s, Schoch and Neugebauer computed that the solar eclipse of 16 April 1178 B.C.E. was total over the Ionian Islands and was the only suitable eclipse in more than a century to agree with classical estimates of the decade-earlier sack of Troy around 1192-1184 B.C.E. However, much skepticism remains about whether the verses refer to this, or any, eclipse. To contribute to the issue independently of the disputed eclipse reference, we analyze other astronomical references in the Epic, without assuming the existence of an eclipse, and search for dates matching the astronomical phenomena we believe they describe. We use three overt astronomical references in the epic: to Boötes and the Pleiades, Venus, and the New Moon; we supplement them with a conjectural identification of Hermes's trip to Ogygia as relating to the motion of planet Mercury. Performing an exhaustive search of all possible dates in the span 1250-1115 B.C., we looked to match these phenomena in the order and manner that the text describes. In that period, a single date closely matches our references: 16 April 1178 B.C.E. We speculate that these references, plus the disputed eclipse reference, may refer to that specific eclipse.

The height-, weight-, and BMI-for-age of Polish school-aged children and adolescents relative to international and local growth references.

PubMed

Kulaga, Zbigniew; Litwin, Mieczysław; Tkaczyk, Marcin; Rózdzyńska, Agnieszka; Barwicka, Katarzyna; Grajda, Aneta; Swiader, Anna; Gurzkowska, Beata; Napieralska, Ewelina; Pan, Huiqi

2010-03-04

The growth of children is an indicator of health and society's wellbeing. Growth references are useful in monitoring a child's growth, which is a very important part of child care. Poland's growth references are not updated regularly. Although several growth reference ranges have been developed in Poland over recent years, sampling was restricted to urban populations of major cities. The aim of this study was to assess how well Polish children match with, or diverge from, regional charts and to compare them with international growth references. Four Polish and two international (WHO 2007 and USCDC2000) growth references were used to calculate the height, weight and BMI z-scores in a recent, large, population-representative sample of school-aged children and adolescents in Poland. The distributions of z-scores were analysed with descriptive and inferential statistical methods. Mean height z-scores calculated with the use of the WHO 2007 and USCDC2000 references were positive and significantly different from zero over the entire age range. The mean height z-score was closest to zero in the Poznan reference for boys (0.05) and Warszawa reference for girls (0.01). Median weight z-scores were positive under all weight references over the entire age range with only the exception of 18-year-old girls' weight z-score calculated relative to USCDC2000. Median BMI z-scores were positive in males in early childhood, decreasing with age. In the case of girls, the median BMI z-score calculated using WHO 2007 and USCDC2000 was close to zero in early childhood, decreased in adolescents and reached minimum values at age 18 years. Median BMI z-scores calculated with the use of the Lodz reference fluctuated between 0.05 and 0.2 over the studied age range. In this contemporary sample of Polish school-aged children, distributions of height, weight and BMI differed from those of children from the international growth references. These differences should be considered when using the references. There exist certain limitations to the analysis of height, weight, and BMI z-scores when Polish regional references are used.

The height-, weight-, and BMI-for-age of Polish school-aged children and adolescents relative to international and local growth references

PubMed Central

2010-01-01

Background The growth of children is an indicator of health and society's wellbeing. Growth references are useful in monitoring a child's growth, which is a very important part of child care. Poland's growth references are not updated regularly. Although several growth reference ranges have been developed in Poland over recent years, sampling was restricted to urban populations of major cities. The aim of this study was to assess how well Polish children match with, or diverge from, regional charts and to compare them with international growth references. Methods Four Polish and two international (WHO 2007 and USCDC2000) growth references were used to calculate the height, weight and BMI z-scores in a recent, large, population-representative sample of school-aged children and adolescents in Poland. The distributions of z-scores were analysed with descriptive and inferential statistical methods. Results Mean height z-scores calculated with the use of the WHO 2007 and USCDC2000 references were positive and significantly different from zero over the entire age range. The mean height z-score was closest to zero in the Poznan reference for boys (0.05) and Warszawa reference for girls (0.01). Median weight z-scores were positive under all weight references over the entire age range with only the exception of 18-year-old girls' weight z-score calculated relative to USCDC2000. Median BMI z-scores were positive in males in early childhood, decreasing with age. In the case of girls, the median BMI z-score calculated using WHO 2007 and USCDC2000 was close to zero in early childhood, decreased in adolescents and reached minimum values at age 18 years. Median BMI z-scores calculated with the use of the Lodz reference fluctuated between 0.05 and 0.2 over the studied age range. Conclusions In this contemporary sample of Polish school-aged children, distributions of height, weight and BMI differed from those of children from the international growth references. These differences should be considered when using the references. There exist certain limitations to the analysis of height, weight, and BMI z-scores when Polish regional references are used. PMID:20199693

[Study on spectrum analysis of X-ray based on rotational mass effect in special relativity].

PubMed

Yu, Zhi-Qiang; Xie, Quan; Xiao, Qing-Quan

2010-04-01

Based on special relativity, the formation mechanism of characteristic X-ray has been studied, and the influence of rotational mass effect on X-ray spectrum has been given. A calculation formula of the X-ray wavelength based upon special relativity was derived. Error analysis was carried out systematically for the calculation values of characteristic wavelength, and the rules of relative error were obtained. It is shown that the values of the calculation are very close to the experimental values, and the effect of rotational mass effect on the characteristic wavelength becomes more evident as the atomic number increases. The result of the study has some reference meaning for the spectrum analysis of characteristic X-ray in application.

Can multi-subpopulation reference sets improve the genomic predictive ability for pigs?

PubMed

Fangmann, A; Bergfelder-Drüing, S; Tholen, E; Simianer, H; Erbe, M

2015-12-01

In most countries and for most livestock species, genomic evaluations are obtained from within-breed analyses. To achieve reliable breeding values, however, a sufficient reference sample size is essential. To increase this size, the use of multibreed reference populations for small populations is considered a suitable option in other species. Over decades, the separate breeding work of different pig breeding organizations in Germany has led to stratified subpopulations in the breed German Large White. Due to this fact and the limited number of Large White animals available in each organization, there was a pressing need for ascertaining if multi-subpopulation genomic prediction is superior compared with within-subpopulation prediction in pigs. Direct genomic breeding values were estimated with genomic BLUP for the trait "number of piglets born alive" using genotype data (Illumina Porcine 60K SNP BeadChip) from 2,053 German Large White animals from five different commercial pig breeding companies. To assess the prediction accuracy of within- and multi-subpopulation reference sets, a random 5-fold cross-validation with 20 replications was performed. The five subpopulations considered were only slightly differentiated from each other. However, the prediction accuracy of the multi-subpopulations approach was not better than that of the within-subpopulation evaluation, for which the predictive ability was already high. Reference sets composed of closely related multi-subpopulation sets performed better than sets of distantly related subpopulations but not better than the within-subpopulation approach. Despite the low differentiation of the five subpopulations, the genetic connectedness between these different subpopulations seems to be too small to improve the prediction accuracy by applying multi-subpopulation reference sets. Consequently, resources should be used for enlarging the reference population within subpopulation, for example, by adding genotyped females.

Capture Versus Capture Zones: Clarifying Terminology Related to Sources of Water to Wells.

PubMed

Barlow, Paul M; Leake, Stanley A; Fienen, Michael N

2018-03-15

The term capture, related to the source of water derived from wells, has been used in two distinct yet related contexts by the hydrologic community. The first is a water-budget context, in which capture refers to decreases in the rates of groundwater outflow and (or) increases in the rates of recharge along head-dependent boundaries of an aquifer in response to pumping. The second is a transport context, in which capture zone refers to the specific flowpaths that define the three-dimensional, volumetric portion of a groundwater flow field that discharges to a well. A closely related issue that has become associated with the source of water to wells is streamflow depletion, which refers to the reduction in streamflow caused by pumping, and is a type of capture. Rates of capture and streamflow depletion are calculated by use of water-budget analyses, most often with groundwater-flow models. Transport models, particularly particle-tracking methods, are used to determine capture zones to wells. In general, however, transport methods are not useful for quantifying actual or potential streamflow depletion or other types of capture along aquifer boundaries. To clarify the sometimes subtle differences among these terms, we describe the processes and relations among capture, capture zones, and streamflow depletion, and provide proposed terminology to distinguish among them. Published 2018. This article is a U.S. Government work and is in the public domain in the USA. Groundwater published by Wiley Periodicals, Inc. on behalf of National Ground Water Association.

Innovative monitoring campaign of the environmental conditions of the Stibbert museum in Florence

NASA Astrophysics Data System (ADS)

Angelini, E.; Civita, F.; Corbellini, S.; Fulginiti, D.; Giovagnoli, A.; Grassini, S.; Parvis, M.

2016-02-01

Conservation of ancient metallic artefact displayed inside museums is a complex problem due to the large number of constraints mainly related to the artefacts fruition by people. The development of a simple procedure for monitoring the artefact conservation state promptly highlighting risky conditions without impacting on the normal museum operations could be of interest in the cultural heritage world. This paper describes the interesting results obtained by using a highly sensitive and innovative methodology for evaluating the safety level of the museum indoor areas, and more specifically of the interior of the showcases, with respect to the metallic artefacts. The methodology is based on the use of an innovative smart sensors network and of copper reference samples. The smart sensors network was employed for the continuous monitoring of temperature and relative humidity close to the artefacts, i.e. inside the display showcases. The reference specimens were Cu coated with a 100 nm Cu nanostructured layer put for 1 year in the exhibition rooms inside and outside the showcases and characterised by means of normal imaging, colorimetric and FESEM techniques at regular intervals. The results of the monitoring activity evidenced the higher reactivity to the environmental aggressivity of the nanocoated copper specimen with respect to bulk artefacts and therefore the possibility to use them as alerts to possible corrosion phenomena that may occur to the real artefacts. A proper temperature and relative humidity monitoring inside the showcases and close to each group of artefacts is a powerful though economic and non-invasive way to highlight most of the possible critical display conditions.

Fish-based indicators of estuarine condition that do not require reference data

NASA Astrophysics Data System (ADS)

Tweedley, James R.; Warwick, Richard M.; Hallett, Chris S.; Potter, Ian C.

2017-05-01

The species composition of fish communities in 15 microtidal estuaries in south-western Australia, ranging from permanently-open to normally-closed, is shown to be related to the geomorphological and hydrological regimes and not to environmental condition. This study then explored the effectiveness of using qualitative taxonomic distinctness and ABC curves for fish data as indicators of the environmental condition in nearshore, shallow waters of these estuaries and, in the case of taxonomic distinctness, also of their offshore, deeper waters. Neither of these indices require spatial or temporal reference data, which may be either prohibitively expensive and time-consuming to collect or unavailable. Taxonomic distinctness, in both nearshore and offshore waters, varied consistently among estuaries in relation to their recorded environmental status, and is thus a good indicator of overall estuarine condition. ABC analyses, however, did not prove a good measure of the environmental condition of the estuaries, because their results largely reflect differences in accessibility of the estuary to marine estuarine-opportunist species and especially those that grow to a larger size. It is concluded that taxonomic distinctness indices provide a rapid and cost-effective method for assessing the environmental condition of estuaries, particularly those with limited spatial or temporal reference data.

Self-evaluated and Close Relative-Evaluated Epworth Sleepiness Scale vs. Multiple Sleep Latency Test in Patients with Obstructive Sleep Apnea

PubMed Central

Li, Yun; Zhang, Jihui; Lei, Fei; Liu, Hong; Li, Zhe; Tang, Xiangdong

2014-01-01

Objectives: The aims of this study were to determine (1) the agreement in Epworth Sleepiness Scale (ESS) evaluated by patients and their close relatives (CRs), and (2) the correlation of objective sleepiness as measured by multiple sleep latency test (MSLT) with self-evaluated and close relative-evaluated ESS. Methods: A total of 85 consecutive patients with obstructive sleep apnea (OSA) (70 males, age 46.7 ± 12.9 years old) with an apnea-hypopnea index (AHI) > 5 events per hour (mean 38.9 ± 26.8/h) were recruited into this study. All participants underwent an overnight polysomnographic assessment (PSG), MSLT, and ESS rated by both patients and their CRs. Mean sleep latency < 8 min on MSLT was considered objective daytime sleepiness. Results: Self-evaluated global ESS score (ESSG) was closely correlated with evaluation by CRs (r = 0.79, p < 0.001); the mean ESSG score evaluated by patients did not significantly differ from that evaluated by CRs (p > 0.05). However, Bland- Altman plot showed individual differences between self-evaluated and CR-evaluated ESS scores, with a 95%CI of -9.3 to 7.0. The mean sleep latency on MSLT was significantly associated with CR-evaluated ESSG (r = -0.23, p < 0.05); significance of association with self-evaluated ESSG was marginal (r = -0.21, p = 0.05). Conclusions: CR-evaluated ESS has a good correlation but also significant individual disagreement with self-evaluated ESS in Chinese patients with OSA. CR-evaluated ESS performs as well as, if not better than, self-evaluated ESS in this population when referring to MSLT. Citation: Li Y; Zhang J; Lei F; Liu H; Li Z; Tang X. Self-evaluated and close relative-evaluated Epworth Sleepiness Scale vs. multiple sleep latency test in patients with obstructive sleep apnea. J Clin Sleep Med 2014;10(2):171-176. PMID:24533000

Acoustical numerology and lucky equal temperaments

NASA Astrophysics Data System (ADS)

Hall, Donald E.

1988-04-01

Equally tempered musical scales with N steps per octave are known to work especially well in approximating justly tuned intervals for such values as N=12, 19, 31, and 53. A quantitative measure of the closeness of such fits is suggested, in terms of the probabilities of coming as close to randomly chosen intervals as to the justly tuned targets. When two or more harmonic intervals are considered simultaneously, this involves a Monte Carlo evaluation of the probabilities. The results can be used to gauge how much advantage the special values of N mentioned above have over others. This article presents the rationale and method of computation, together with illustrative results in a few of the most interesting cases. References are provided to help relate these results to earlier works by music theorists.

Transitioning Advanced Concept Technology Demonstrations to Acquisition Programs

DTIC Science & Technology

2003-12-01

for the first time, based on UMS reports. In addition, the RMWS portion of the UGC ACTD program, through close coordination with multiple users...reference a) X X X Market Research 10 U.S.C. §2377 (reference b) X X X Acquisition Program Baseline (APB) 10 U.S.C. §2435 (reference c

Statistical clumped isotope signatures

PubMed Central

Röckmann, T.; Popa, M. E.; Krol, M. C.; Hofmann, M. E. G.

2016-01-01

High precision measurements of molecules containing more than one heavy isotope may provide novel constraints on element cycles in nature. These so-called clumped isotope signatures are reported relative to the random (stochastic) distribution of heavy isotopes over all available isotopocules of a molecule, which is the conventional reference. When multiple indistinguishable atoms of the same element are present in a molecule, this reference is calculated from the bulk (≈average) isotopic composition of the involved atoms. We show here that this referencing convention leads to apparent negative clumped isotope anomalies (anti-clumping) when the indistinguishable atoms originate from isotopically different populations. Such statistical clumped isotope anomalies must occur in any system where two or more indistinguishable atoms of the same element, but with different isotopic composition, combine in a molecule. The size of the anti-clumping signal is closely related to the difference of the initial isotope ratios of the indistinguishable atoms that have combined. Therefore, a measured statistical clumped isotope anomaly, relative to an expected (e.g. thermodynamical) clumped isotope composition, may allow assessment of the heterogeneity of the isotopic pools of atoms that are the substrate for formation of molecules. PMID:27535168

Behavioral economics

PubMed Central

Hursh, Steven R.

1984-01-01

Economics, like behavioral psychology, is a science of behavior, albeit highly organized human behavior. The value of economic concepts for behavioral psychology rests on (1) their empirical validity when tested in the laboratory with individual subjects and (2) their uniqueness when compared to established behavioral concepts. Several fundamental concepts are introduced and illustrated by reference to experimental data: open and closed economies, elastic and inelastic demand, and substitution versus complementarity. Changes in absolute response rate are analyzed in relation to elasticity and intensity of demand. The economic concepts of substitution and complementarity are related to traditional behavioral studies of choice and to the matching relation. The economic approach has many implications for the future of behavioral research and theory. In general, economic concepts are grounded on a dynamic view of reinforcement. The closed-economy methodology extends the generality of behavioral principles to situations in which response rate and obtained rate of reinforcement are interdependent. Analysis of results in terms of elasticity and intensity of demand promises to provide a more direct method for characterizing the effects of “motivational” variables. Future studies of choice should arrange heterogeneous reinforcers with varying elasticities, use closed economies, and modulate scarcity or income. The economic analysis can be extended to the study of performances that involve subtle discriminations or skilled movements that vary in accuracy or quality as opposed to rate or quantity, and thus permit examination of time/accuracy trade-offs. PMID:16812401

Application of permanents of square matrices for DNA identification in multiple-fatality cases

PubMed Central

2013-01-01

Background DNA profiling is essential for individual identification. In forensic medicine, the likelihood ratio (LR) is commonly used to identify individuals. The LR is calculated by comparing two hypotheses for the sample DNA: that the sample DNA is identical or related to a reference DNA, and that it is randomly sampled from a population. For multiple-fatality cases, however, identification should be considered as an assignment problem, and a particular sample and reference pair should therefore be compared with other possibilities conditional on the entire dataset. Results We developed a new method to compute the probability via permanents of square matrices of nonnegative entries. As the exact permanent is known as a #P-complete problem, we applied the Huber–Law algorithm to approximate the permanents. We performed a computer simulation to evaluate the performance of our method via receiver operating characteristic curve analysis compared with LR under the assumption of a closed incident. Differences between the two methods were well demonstrated when references provided neither obligate alleles nor impossible alleles. The new method exhibited higher sensitivity (0.188 vs. 0.055) at a threshold value of 0.999, at which specificity was 1, and it exhibited higher area under a receiver operating characteristic curve (0.990 vs. 0.959, P = 9.6E-15). Conclusions Our method therefore offers a solution for a computationally intensive assignment problem and may be a viable alternative to LR-based identification for closed-incident multiple-fatality cases. PMID:23962363

Effect of Spatial Locality Prefetching on Structural Locality

DTIC Science & Technology

1991-12-01

Pollution module calculates the SLC and CAM cache pollution percentages. And finally, the Generate Reference Frequency List module produces the output...3.2.5 Generate Reference Frequency List 3.2.6 Each program module in the structure chart is mapped into an Ada package. By performing this encapsulation...call routine to generate reference -- frequency list -- end if -- end loop -- close input, output, and reference files end Cache Simulator Figure 3.5

Pneumatic pressure wave generator provides economical, simple testing of pressure transducers

NASA Technical Reports Server (NTRS)

Gaal, A. E.; Weldon, T. P.

1967-01-01

Testing device utilizes the change in pressure about a bias or reference pressure level produced by displacement of a center-driven piston in a closed cylinder. Closely controlled pneumatic pressure waves allow testing under dynamic conditions.

40 CFR 261.21 - Characteristic of ignitability.

Code of Federal Regulations, 2011 CFR

2011-07-01

... determined by a Pensky-Martens Closed Cup Tester, using the test method specified in ASTM Standard D 93-79 or D 93-80 (incorporated by reference, see § 260.11), or a Setaflash Closed Cup Tester, using the test...

40 CFR 261.21 - Characteristic of ignitability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... determined by a Pensky-Martens Closed Cup Tester, using the test method specified in ASTM Standard D 93-79 or D 93-80 (incorporated by reference, see § 260.11), or a Setaflash Closed Cup Tester, using the test...

Is an eclipse described in the Odyssey?

PubMed Central

Baikouzis, Constantino; Magnasco, Marcelo O.

2008-01-01

Plutarch and Heraclitus believed a certain passage in the 20th book of the Odyssey (“Theoclymenus's prophecy”) to be a poetic description of a total solar eclipse. In the late 1920s, Schoch and Neugebauer computed that the solar eclipse of 16 April 1178 B.C.E. was total over the Ionian Islands and was the only suitable eclipse in more than a century to agree with classical estimates of the decade-earlier sack of Troy around 1192–1184 B.C.E. However, much skepticism remains about whether the verses refer to this, or any, eclipse. To contribute to the issue independently of the disputed eclipse reference, we analyze other astronomical references in the Epic, without assuming the existence of an eclipse, and search for dates matching the astronomical phenomena we believe they describe. We use three overt astronomical references in the epic: to Boötes and the Pleiades, Venus, and the New Moon; we supplement them with a conjectural identification of Hermes's trip to Ogygia as relating to the motion of planet Mercury. Performing an exhaustive search of all possible dates in the span 1250–1115 B.C., we looked to match these phenomena in the order and manner that the text describes. In that period, a single date closely matches our references: 16 April 1178 B.C.E. We speculate that these references, plus the disputed eclipse reference, may refer to that specific eclipse. PMID:18577587

Improving the Virtual Reference Experience: How Closely Do Academic Libraries Adhere to RUSA Guidelines?

ERIC Educational Resources Information Center

Platt, Jessica; Benson, Pete

2010-01-01

The purpose of this study was to measure the degree to which academic libraries or library staff members throughout the United States adhere to the Guidelines for Virtual Reference Services provided by the Reference & User Services Association (RUSA). The results of the study were analyzed to identify specific areas where improvement is needed…

Age-related epigenetic drift and phenotypic plasticity loss: implications in prevention of age-related human diseases

PubMed Central

Li, Yuanyuan; Tollefsbol, Trygve O

2016-01-01

Aging is considered as one of the most important developmental processes in organisms and is closely associated with global deteriorations of epigenetic markers such as aberrant methylomic patterns. This altered epigenomic state, referred to ‘epigenetic drift’, reflects deficient maintenance of epigenetic marks and contributes to impaired cellular and molecular functions in aged cells. Epigenetic drift-induced abnormal changes during aging are scantily repaired by epigenetic modulators. This inflexibility in the aged epigenome may lead to an age-related decline in phenotypic plasticity at the cellular and molecular levels due to epigenetic drift. This perspective aims to provide novel concepts for understanding epigenetic effects on the aging process and to provide insights into epigenetic prevention and therapeutic strategies for age-related human disease. PMID:27882781

Evaluation of water quality threats to the endangered Okaloosa darter (Etheostoma okaloosae) in East Turkey Creek on Eglin Air Force Base.

PubMed

Weil, R E; Spade, D J; Knoebl, I; Hemming, J M; Tongue, M L; Szabo, N J; Kroll, K J; Tate, W B; Denslow, N D

2012-04-01

The threatened Okaloosa darter (Etheostoma okaloosae) is found almost exclusively on the Eglin Air Force Base in the Choctawhatchee Bay watershed of Florida. Portions of this limited habitat are threatened with soil erosion, altered hydrology, and impaired water quality. In the present study, general water quality parameters (i.e., dissolved oxygen, specific conductance, pH, temperature, relative turbidity, and primary productivity) were characterized in East Turkey Creek, which is a body of water potentially impacted by treated wastewater sprayfields, and Long Creek, an adjacent reference stream that does not border the sprayfields. Water quality was assessed during a 30-day exposure using passive samplers for both non-polar and polar effluent parameters. Because the Okaloosa darter was listed as endangered at the time of sampling we chose a closely related species from the same creeks, the sailfin shiner (Pteronotropis hypseleotris) in which to measure metal body burdens. Additionally, fathead minnows (Pimephales promelas) were used for microarray analysis on gonad and liver tissues after 48 h exposures to water collected from the two creeks and brought into the laboratory. Waters from all sites, including reference sites, affected the expression of genes related to various biological processes including transcription and translation, cell cycle control, metabolism, and signaling pathways, suggesting that the sum of anthropogenic compounds in the site waters may cause a generalized stress response in both liver and testis, an effect that could be related to the generally low populations of the Okaloosa darter. Furthermore, effects of site waters on fish gene expression may be related to the impact of human activities other than the wastewater sprayfields, as nearby areas are closed to the public for military testing, training, and administrative activities and due to ordnance contamination. Copyright © 2012 Elsevier B.V. All rights reserved.

Reference as an Interactive Achievement: Sequential and Longitudinal Analyses of Labeling Interactions in Shared Book Reading and Free Play.

PubMed

Heller, Vivien; Rohlfing, Katharina J

2017-01-01

The present study examines how young children and their caregivers establish reference by jointly developing stable patterns of bodily, perceptual, and interactive coordination. Our longitudinal investigation focuses on two mother-child dyads engaged in picture-book reading and play. The dyads were videotaped at home once every 6 weeks while the children aged from 9 to 24 months. Inspired by conversation analysis and multimodal analysis, our developmental approach builds on the insight that the situated and embodied production of reference is fundamentally an interactive achievement. To examine the acquisition of reference, we developed a descriptive instrument that takes account of not only the dyad's joint accomplishment but also each participant's contributions to it. The instrument is based on the sequential reconstruction of the jobs that both participants have to accomplish jointly in order to achieve reference: establishing visual perception as a relevant resource, constituting a domain of scrutiny, locating a target, and construing the (meaning of the) referent. Methodologically, these jobs serve as a tertium comparationis for the longitudinal comparison of both the adult's as well as the child's contributions to establishing reference. We used this instrument to examine (1) what bodily and verbal resources the participants employed, and (2) how their contributions to accomplishing the jobs changed over time. Findings showed that the acquisition of reference was closely related to the child's increasing ability to recognize, fulfill, and set up conditional relevancies. We conclude that the adult's dynamic and contextualized use of conditional relevancies, recipient design, and observability is a crucial driving force in the acquisition of reference.

Role and Variation of the Amount and Composition of Glomalin in Soil Properties in Farmland and Adjacent Plantations with Reference to a Primary Forest in North-Eastern China

PubMed Central

Wang, Qiong; Wang, Wenjie; He, Xingyuan; Zhang, Wentian; Song, Kaishan; Han, Shijie

2015-01-01

The glycoprotein known as glomalin-related soil protein (GRSP) is abundantly produced on the hyphae and spores of arbuscular mycorrhizal fungi (AMF) in soil and roots. Few studies have focused on its amount, composition and associations with soil properties and possible land-use influences, although the data hints at soil rehabilitation. By choosing a primary forest soil as a non-degraded reference, it is possible to explore whether afforestation can improve degraded farmland soil by altering GRSP. In this paper, close correlations were found between various soil properties (soil organic carbon, nitrogen, pH, electrical conductivity (EC), and bulk density) and the GRSP amount, between various soil properties and GRSP composition (main functional groups, fluorescent substances, and elements). Afforestation on farmland decreased the EC and bulk density (p < 0.05). The primary forest had a 2.35–2.56-fold higher GRSP amount than those in the plantation forest and farmland, and GRSP composition (tryptophan-like and fulvic acid-like fluorescence; functional groups of C–H, C–O, and O–H; elements of Al, O, Si, C, Ca, and N) in primary forest differed from those in plantation forest and farmland (p < 0.05). However, no evident differences in GRSP amount and composition were observed between the farmland and the plantation forest. Our finding highlights that 30 years poplar afforestation on degraded farmland is not enough to change GRSP-related properties. A longer period of afforestation with close-to-nature managements may favor the AMF-related underground recovery processes. PMID:26430896

Delineation of the Species Haemophilus influenzae by Phenotype, Multilocus Sequence Phylogeny, and Detection of Marker Genes▿ †

PubMed Central

Nørskov-Lauritsen, Niels; Overballe, Merete D.; Kilian, Mogens

2009-01-01

To obtain more information on the much-debated definition of prokaryotic species, we investigated the borders of Haemophilus influenzae by comparative analysis of H. influenzae reference strains with closely related bacteria including strains assigned to Haemophilus haemolyticus, cryptic genospecies biotype IV, and the never formally validated species “Haemophilus intermedius”. Multilocus sequence phylogeny based on six housekeeping genes separated a cluster encompassing the type and the reference strains of H. influenzae from 31 more distantly related strains. Comparison of 16S rRNA gene sequences supported this delineation but was obscured by a conspicuously high number of polymorphic sites in many of the strains that did not belong to the core group of H. influenzae strains. The division was corroborated by the differential presence of genes encoding H. influenzae adhesion and penetration protein, fuculokinase, and Cu,Zn-superoxide dismutase, whereas immunoglobulin A1 protease activity or the presence of the iga gene was of limited discriminatory value. The existence of porphyrin-synthesizing strains (“H. intermedius”) closely related to H. influenzae was confirmed. Several chromosomally encoded hemin biosynthesis genes were identified, and sequence analysis showed these genes to represent an ancestral genotype rather than recent transfers from, e.g., Haemophilus parainfluenzae. Strains previously assigned to H. haemolyticus formed several separate lineages within a distinct but deeply branching cluster, intermingled with strains of “H. intermedius” and cryptic genospecies biotype IV. Although H. influenzae is phenotypically more homogenous than some other Haemophilus species, the genetic diversity and multicluster structure of strains traditionally associated with H. influenzae make it difficult to define the natural borders of that species. PMID:19060144

H∞ output tracking control of discrete-time nonlinear systems via standard neural network models.

PubMed

Liu, Meiqin; Zhang, Senlin; Chen, Haiyang; Sheng, Weihua

2014-10-01

This brief proposes an output tracking control for a class of discrete-time nonlinear systems with disturbances. A standard neural network model is used to represent discrete-time nonlinear systems whose nonlinearity satisfies the sector conditions. H∞ control performance for the closed-loop system including the standard neural network model, the reference model, and state feedback controller is analyzed using Lyapunov-Krasovskii stability theorem and linear matrix inequality (LMI) approach. The H∞ controller, of which the parameters are obtained by solving LMIs, guarantees that the output of the closed-loop system closely tracks the output of a given reference model well, and reduces the influence of disturbances on the tracking error. Three numerical examples are provided to show the effectiveness of the proposed H∞ output tracking design approach.

Closed-loop analysis and control of a non-inverting buck-boost converter

NASA Astrophysics Data System (ADS)

Chen, Zengshi; Hu, Jiangang; Gao, Wenzhong

2010-11-01

In this article, a cascade controller is designed and analysed for a non-inverting buck-boost converter. The fast inner current loop uses sliding mode control. The slow outer voltage loop uses the proportional-integral (PI) control. Stability analysis and selection of PI gains are based on the nonlinear closed-loop error dynamics incorporating both the inner and outer loop controllers. The closed-loop system is proven to have a nonminimum phase structure. The voltage transient due to step changes of input voltage or resistance is predictable. The operating range of the reference voltage is discussed. The controller is validated by a simulation circuit. The simulation results show that the reference output voltage is well-tracked under system uncertainties or disturbances, confirming the validity of the proposed controller.

Development of a Polymerase Chain Reaction Assay for the Specific Identification of Burkholderia mallei and Differentiation from Burkholderia pseudomallei and Other Closely Related Burkholderiaceae

DTIC Science & Technology

2005-11-29

used in this work were grown in LB broth. C . violaceum and Y. pestis were propagated at 25 8C, whereas the remaining bacterial isolates were cultured...reference panel, which includ- ed B. cepacia, B. cenocepacia, B. stabilis, B. multivorans, B. vietnamiensis, E. coli, C . violaceum , Y. pestis, and P...19a. NAME OF RESPONSIBLE PERSON a. REPORT unclassified b. ABSTRACT unclassified c . THIS PAGE unclassified Standard Form 298 (Rev. 8-98

Recent advances on polyoxometalate-based molecular and composite materials.

PubMed

Song, Yu-Fei; Tsunashima, Ryo

2012-11-21

Polyoxometalates (POMs) are a subset of metal oxides with unique physical and chemical properties, which can be reliably modified through various techniques and methods to develop sophisticated materials and devices. In parallel with the large number of new crystal structures reported in the literature, the application of these POMs towards multifunctional materials has attracted considerable attention. This critical review summarizes recent progress on POM-based molecular and composite materials, and particularly highlights the emerging areas that are closely related to surface, electronic, energy, environment, life science, etc. (171 references).

National Economic Development Procedures Manual - Recreation. Volume 2. A Guide for Using the Contingent Value Methodology in Recreation Studies,

DTIC Science & Technology

1986-03-01

Directly from Sample Bid VI-16 Example 3 VI-16 Determining the Zero Price Qiantity Demanded VI-26 Summary VI -31 CHAPrER VII, THE DETERMINATION OF NED...While the standard deviation and variance are absolute measures of dispersion, a relative measure of dispersion can also be computed. This measure is...refers to the closeness of fit between the estimates obtained from Zli e and the true population value. The only way of being absolutely i: o-.iat the

An observational analysis: Tropical relative to Arctic influence on midlatitude weather in the era of Arctic amplification

NASA Astrophysics Data System (ADS)

Cohen, Judah

2016-05-01

The tropics, in general, and El Niño/Southern Oscillation (ENSO) in particular are almost exclusively relied upon for seasonal forecasting. Much less considered and certainly more controversial is the idea that Arctic variability is influencing midlatitude weather. However, since the late 1980s and early 1990s, the Arctic has undergone the most rapid warming observed globally, referred to as Arctic amplification (AA), which has coincided with an observed increase in extreme weather. Analysis of observed trends in hemispheric circulation over the period of AA more closely resembles variability associated with Arctic boundary forcings than with tropical forcing. Furthermore, analysis of intraseasonal temperature variability shows that the cooling in midlatitude winter temperatures has been accompanied by an increase in temperature variability and not a decrease, popularly referred to as "weather whiplash."

Evaluating the accuracy and large inaccuracy of two continuous glucose monitoring systems.

PubMed

Leelarathna, Lalantha; Nodale, Marianna; Allen, Janet M; Elleri, Daniela; Kumareswaran, Kavita; Haidar, Ahmad; Caldwell, Karen; Wilinska, Malgorzata E; Acerini, Carlo L; Evans, Mark L; Murphy, Helen R; Dunger, David B; Hovorka, Roman

2013-02-01

This study evaluated the accuracy and large inaccuracy of the Freestyle Navigator (FSN) (Abbott Diabetes Care, Alameda, CA) and Dexcom SEVEN PLUS (DSP) (Dexcom, Inc., San Diego, CA) continuous glucose monitoring (CGM) systems during closed-loop studies. Paired CGM and plasma glucose values (7,182 data pairs) were collected, every 15-60 min, from 32 adults (36.2±9.3 years) and 20 adolescents (15.3±1.5 years) with type 1 diabetes who participated in closed-loop studies. Levels 1, 2, and 3 of large sensor error with increasing severity were defined according to absolute relative deviation greater than or equal to ±40%, ±50%, and ±60% at a reference glucose level of ≥6 mmol/L or absolute deviation greater than or equal to ±2.4 mmol/L,±3.0 mmol/L, and ±3.6 mmol/L at a reference glucose level of <6 mmol/L. Median absolute relative deviation was 9.9% for FSN and 12.6% for DSP. Proportions of data points in Zones A and B of Clarke error grid analysis were similar (96.4% for FSN vs. 97.8% for DSP). Large sensor over-reading, which increases risk of insulin over-delivery and hypoglycemia, occurred two- to threefold more frequently with DSP than FSN (once every 2.5, 4.6, and 10.7 days of FSN use vs. 1.2, 2.0, and 3.7 days of DSP use for Level 1-3 errors, respectively). At levels 2 and 3, large sensor errors lasting 1 h or longer were absent with FSN but persisted with DSP. FSN and DSP differ substantially in the frequency and duration of large inaccuracy despite only modest differences in conventional measures of numerical and clinical accuracy. Further evaluations are required to confirm that FSN is more suitable for integration into closed-loop delivery systems.

Close-range hyperspectral image analysis for the early detection of stress responses in individual plants in a high-throughput phenotyping platform

NASA Astrophysics Data System (ADS)

Mohd Asaari, Mohd Shahrimie; Mishra, Puneet; Mertens, Stien; Dhondt, Stijn; Inzé, Dirk; Wuyts, Nathalie; Scheunders, Paul

2018-04-01

The potential of close-range hyperspectral imaging (HSI) as a tool for detecting early drought stress responses in plants grown in a high-throughput plant phenotyping platform (HTPPP) was explored. Reflectance spectra from leaves in close-range imaging are highly influenced by plant geometry and its specific alignment towards the imaging system. This induces high uninformative variability in the recorded signals, whereas the spectral signature informing on plant biological traits remains undisclosed. A linear reflectance model that describes the effect of the distance and orientation of each pixel of a plant with respect to the imaging system was applied. By solving this model for the linear coefficients, the spectra were corrected for the uninformative illumination effects. This approach, however, was constrained by the requirement of a reference spectrum, which was difficult to obtain. As an alternative, the standard normal variate (SNV) normalisation method was applied to reduce this uninformative variability. Once the envisioned illumination effects were eliminated, the remaining differences in plant spectra were assumed to be related to changes in plant traits. To distinguish the stress-related phenomena from regular growth dynamics, a spectral analysis procedure was developed based on clustering, a supervised band selection, and a direct calculation of a spectral similarity measure against a reference. To test the significance of the discrimination between healthy and stressed plants, a statistical test was conducted using a one-way analysis of variance (ANOVA) technique. The proposed analysis techniques was validated with HSI data of maize plants (Zea mays L.) acquired in a HTPPP for early detection of drought stress in maize plant. Results showed that the pre-processing of reflectance spectra with the SNV effectively reduces the variability due to the expected illumination effects. The proposed spectral analysis method on the normalized spectra successfully detected drought stress from the third day of drought induction, confirming the potential of HSI for drought stress detection studies and further supporting its adoption in HTPPP.

Family influences on the cognitive development of profoundly deaf children: exploring the effects of socioeconomic status and siblings.

PubMed

Macaulay, Catrin E; Ford, Ruth M

2013-10-01

We evaluated the cognitive development of 48 profoundly deaf children from hearing families (born 1994-2002, mean age M = 8.0 years at time of test, none of whom had received early auditory-verbal therapy) as a function of family socioeconomic status and number of siblings. Overall, the deaf children matched a younger group of 47 hearing controls (M = 4.6 years) on verbal ability, theory of mind, and cognitive inhibition. Partial correlations (controlling for age) revealed positive relations in the hearing group between maternal education and inhibition, between number of younger siblings and references to emotions, and between number of close-in-age siblings and references to desires and false beliefs. In the deaf group, there were positive relations between household income and memory span, between maternal education and references to false beliefs, and between number of younger siblings and nonverbal ability. In contrast, deaf children with a greater number of older siblings aged ≤12 years showed inferior memory span, inhibition, belief understanding, picture-sequencing accuracy, and mental-state language, suggesting that they failed to compete successfully with older siblings for their parents' attention and material resources. We consider the implications of the findings for understanding birth-order effects on deaf and language-impaired children.

Quantification of anhydride groups in anhydride-based epoxy hardeners by reaction headspace gas chromatography.

PubMed

Xie, Wei-Qi; Gong, Yi-Xian; Yu, Kong-Xian

2017-06-01

We demonstrate a reaction headspace gas chromatographic method for quantifying anhydride groups in anhydride-based epoxy hardeners. In this method, the conversion process of anhydride groups can be realized by two steps. In the first step, anhydride groups in anhydride-based epoxy hardeners completely reacted with water to form carboxyl groups. In the second step, the carboxyl groups reacted with sodium bicarbonate solution in a closed sample vial. After the complete reaction between the carboxyl groups and sodium bicarbonate, the CO 2 formed from this reaction was then measured by headspace gas chromatography. The data showed that the reaction in the closed headspace vial can be completed in 15 min at 55°C, the relative standard deviation of the reaction headspace gas chromatography method in the precision test was less than 3.94%, the relative differences between the new method and a reference method were no more than 9.38%. The present reaction method is automated, efficient and can be a reliable tool for quantifying the anhydride groups in anhydride-based epoxy hardeners and related research. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Comparative bioinformatics analyses and profiling of lysosome-related organelle proteomes

NASA Astrophysics Data System (ADS)

Hu, Zhang-Zhi; Valencia, Julio C.; Huang, Hongzhan; Chi, An; Shabanowitz, Jeffrey; Hearing, Vincent J.; Appella, Ettore; Wu, Cathy

2007-01-01

Complete and accurate profiling of cellular organelle proteomes, while challenging, is important for the understanding of detailed cellular processes at the organelle level. Mass spectrometry technologies coupled with bioinformatics analysis provide an effective approach for protein identification and functional interpretation of organelle proteomes. In this study, we have compiled human organelle reference datasets from large-scale proteomic studies and protein databases for seven lysosome-related organelles (LROs), as well as the endoplasmic reticulum and mitochondria, for comparative organelle proteome analysis. Heterogeneous sources of human organelle proteins and rodent homologs are mapped to human UniProtKB protein entries based on ID and/or peptide mappings, followed by functional annotation and categorization using the iProXpress proteomic expression analysis system. Cataloging organelle proteomes allows close examination of both shared and unique proteins among various LROs and reveals their functional relevance. The proteomic comparisons show that LROs are a closely related family of organelles. The shared proteins indicate the dynamic and hybrid nature of LROs, while the unique transmembrane proteins may represent additional candidate marker proteins for LROs. This comparative analysis, therefore, provides a basis for hypothesis formulation and experimental validation of organelle proteins and their functional roles.

Focus control system for stretched-membrane mirror module

DOEpatents

Butler, B.L.; Beninga, K.J.

1991-05-21

A focus control system dynamically sets and controls the focal length of a reflective membrane supported between a perimeter frame. A rear membrane is also supported between the perimeter frame rearward and spaced apart from a back side of the reflective membrane. The space between the membranes defines a plenum space into which a mass of gas at a first pressure is inserted. The pressure differential between the first pressure and an external pressure, such as the atmospheric pressure, causes the reflective membrane to assume a first curvature relative to a reference plane associated with the perimeter frame. This curvature defines the focal length of the reflective membrane. The focal length is dynamically controlled by changing the volume of the plenum space, thereby changing the first pressure. The system can be used to change or maintain the pressure differential and hence the front membrane curvature. The plenum volume is changed by pushing or pulling on a central section of the rear membrane using a suitable actuator. Sensing means continuously sense the location of the reflective membrane relative to the reference plane. This sensed position is compared to a reference position, and a resulting error signal, comprising the difference between the sensed position and reference position, drives the actuator in a direction to minimize the difference. A vent value compensates for temperature changes or leaks in the closed volume by allowing the pressure differential to be adjusted as required to center the working range of the actuator about the desired focal length. 13 figures.

Focus control system for stretched-membrane mirror module

DOEpatents

Butler, Barry L.; Beninga, Kelly J.

1991-01-01

A focus control system dynamically sets and controls the focal length of a reflective membrane supported between a perimeter frame. A rear membrane is also supported between the perimeter frame rearward and spaced apart from a back side of the reflective membrane. The space between the membranes defines a plenum space into which a mass of gas at a first pressure is inserted. The pressure differential between the first pressure and an external pressure, such as the atmospheric pressure, causes the reflective membrane to assume a first curvature relative to a reference plane associated with the perimeter frame. This curvature defines the focal length of the reflective membrane. The focal length is dynamically controlled by changing the volume of the plenum space, thereby changing the first pressure. The system can be used to change or maintain the pressure differential and hence the front membrane curvature. The plenum volume is changed by pushing or pulling on a central section of the rear membrane using a suitable actuator. Sensing means continuously sense the location of the reflective membrane relative to the reference plane. This sensed position is compared to a reference position, and a resulting error signal, comprising the difference between the sensed position and reference position, drives the actuator in a direction to minimize the difference. A vent value compensates for temperature changes or leaks in the closed volume by allowing the pressure differential to be adjusted as required to center the working range of the actuator about the desired focal length.

Influence of Ametropia and Its Correction on Measurement of Accommodation.

PubMed

Bernal-Molina, Paula; Vargas-Martín, Fernando; Thibos, Larry N; López-Gil, Norberto

2016-06-01

Amplitude of accommodation (AA) is reportedly greater for myopic eyes than for hyperopic eyes. We investigated potential explanations for this difference. Analytical analysis and computer ray tracing were performed on two schematic eye models of axial ametropia. Using paraxial and nonparaxial approaches, AA was specified for the naked and the corrected eye using the anterior corneal surface as the reference plane. Assuming that axial myopia is due entirely to an increase in vitreous chamber depth, AA increases with the amount of myopia for two reasons that have not always been taken into account. First is the choice of reference location for specifying refractive error and AA in diopters. When specified relative to the cornea, AA increases with the degree of myopia more than when specified relative to the eye's first Gaussian principal plane. The second factor is movement of the eye's second Gaussian principal plane toward the retina during accommodation, which has a larger dioptric effect in shorter eyes. Using the corneal plane (placed at the corneal vertex) as the reference plane for specifying accommodation, AA depends slightly on the axial length of the eye's vitreous chamber. This dependency can be reduced significantly by using a reference plane located 4 mm posterior to the corneal plane. A simple formula is provided to help clinicians and researchers obtain a value of AA that closely reflects power changes of the crystalline lens, independent of axial ametropia and its correction with lenses.

A population-based study of familial Alzheimer disease: Linkage to chromosomes 14, 19, and 21

DOE Office of Scientific and Technical Information (OSTI.GOV)

Duijn, C.M. van; Hofman, A.; Hendriks, L.

1994-10-01

Linkage of Alzheimer disease (AD) to DNA markers on chromosomes 14, 19, and 21 was studied in 10 families in which the disease was apparently inherited as an autosomal dominant trait. Families were derived from a Dutch population-based epidemiologic study of early-onset AD. Although in all probands the onset of AD was at or before age 65 years, the mean age at onset was after age 65 years in four families (referred to as {open_quotes}LOAD{close_quotes}). Among the six families with early-onset AD (referred to as {open_quotes}EOAD,{close_quotes} i.e., mean age of onset of AD of relatives was at or before agemore » 65 years), conclusive linkage to 14q24.3 was found in one family with a very early onset (around 47 years), while linkage to the same region was excluded in two other families. For the LOAD families, predominantly negative lod scores were obtained, and the overall lod score excluded linkage to chromosome 14. The results with markers on chromosome 19 and chromosome 21 were not conclusive for EOAD and LOAD. The findings of our study confirm genetic heterogeneity within familial EOAD. 50 refs., 7 figs., 2 tabs.« less

Solid state safety jumper cables

DOEpatents

Kronberg, James W.

1993-01-01

Solid state jumper cables for connecting two batteries in parallel, having two bridge rectifiers for developing a reference voltage, a four-input decoder for determining which terminals are to be connected based on a comparison of the voltage at each of the four terminals to the reference voltage, and a pair of relays for effecting the correct connection depending on the determination of the decoder. No connection will be made unless only one terminal of each battery has a higher voltage than the reference voltage, indicating "positive" terminals, and one has a lower voltage than the reference voltage, indicating "negative" terminals, and that, therefore, the two high voltage terminals may be connected and the two lower voltage terminals may be connected. Current flows once the appropriate relay device is closed. The relay device is preferably a MOSFET (metal oxide semiconductor field effect transistor) combined with a series array of photodiodes that develop MOSFET gate-closing potential when the decoder output causes an LED to light.

Solid state safety jumper cables

DOEpatents

Kronberg, J.W.

1993-02-23

Solid state jumper cables for connecting two batteries in parallel, having two bridge rectifiers for developing a reference voltage, a four-input decoder for determining which terminals are to be connected based on a comparison of the voltage at each of the four terminals to the reference voltage, and a pair of relays for effecting the correct connection depending on the determination of the decoder. No connection will be made unless only one terminal of each battery has a higher voltage than the reference voltage, indicating positive'' terminals, and one has a lower voltage than the reference voltage, indicating negative'' terminals, and that, therefore, the two high voltage terminals may be connected and the two lower voltage terminals may be connected. Current flows once the appropriate relay device is closed. The relay device is preferably a MOSFET (metal oxide semiconductor field effect transistor) combined with a series array of photodiodes that develop MOSFET gate-closing potential when the decoder output causes an LED to light.

Bronchoalveolar lavage for diagnosis of tuberculosis infection in elephants.

PubMed

Hermes, R; Saragusty, J; Moser, I; Holtze, S; Nieter, J; Sachse, K; Voracek, T; Bernhard, A; Bouts, T; Göritz, F; Hildebrandt, T B

2018-03-01

Tuberculosis (TB) has been known to affect elephants for thousands of years. It was put into spotlight when few circus elephants were diagnosed carrying Mycobacterium (M.) tuberculosis. Because of the zoonotic risk and high susceptibility to M. tuberculosis, periodic testing was enacted since, in captive breeding programmes. Presently, trunk wash is the recommended diagnostic procedure for TB. Trunk wash, however, puts the operator at risk, has low sensitivity, and is prone to contamination. Here, bronchoalveolar lavage is described for the first time for TB diagnosis in elephants. Bronchial, trunk and mouth fluids were investigated using bacterial culture, M. tuberculosis complex (MTC)-specific real-time quantitative PCR (qPCR) and mycobacterial genus-specific qPCR for overall presence of mycobacteria or mycobacterial DNA including bacteria or DNA of closely related genera, respectively, in 14 elephants. Neither bacteria of the MTC nor their DNA were identified in any of the elephants. Yet, 25% of the cultures grew non-tuberculous mycobacteria (NTM) or closely related bacterial species. Furthermore, 85% of the samples contained DNA of NTM or closely related bacterial genera. This finding might explain continued false-positive results from various serological tests. From a zoonotic point of view, bronchoalveolar lavage is safer for the testing personal, has higher probability of capturing MTC and, through PCR, identifies DNA NTM in elephants. Yet, necessary endoscopic equipment, animal sedation and access to a TB reference laboratory might pose challenging requirements in remote conditions in some elephant range countries.

Divergence of actual and reference evapotranspiration observations for irrigated sugarcane with windy tropical conditions

NASA Astrophysics Data System (ADS)

Anderson, R. G.; Wang, D.; Tirado-Corbalá, R.; Zhang, H.; Ayars, J. E.

2015-01-01

Standardized reference evapotranspiration (ET) and ecosystem-specific vegetation coefficients are frequently used to estimate actual ET. However, equations for calculating reference ET have not been well validated in tropical environments. We measured ET (ETEC) using eddy covariance (EC) towers at two irrigated sugarcane fields on the leeward (dry) side of Maui, Hawaii, USA in contrasting climates. We calculated reference ET at the fields using the short (ET0) and tall (ETr) vegetation versions of the American Society for Civil Engineers (ASCE) equation. The ASCE equations were compared to the Priestley-Taylor ET (ETPT) and ETEC. Reference ET from the ASCE approaches exceeded ETEC during the mid-period (when vegetation coefficients suggest ETEC should exceed reference ET). At the windier tower site, cumulative ETr exceeded ETEC by 854 mm over the course of the mid-period (267 days). At the less windy site, mid-period ETr still exceeded ETEC, but the difference was smaller (443 mm). At both sites, ETPT approximated mid-period ETEC more closely than the ASCE equations ((ETPT-ETEC) < 170 mm). Analysis of applied water and precipitation, soil moisture, leaf stomatal resistance, and canopy cover suggest that the lower observed ETEC was not the result of water stress or reduced vegetation cover. Use of a custom-calibrated bulk canopy resistance improved the reference ET estimate and reduced seasonal ET discrepancy relative to ETPT and ETEC in the less windy field and had mixed performance in the windier field. These divergences suggest that modifications to reference ET equations may be warranted in some tropical regions.

Divergence of reference evapotranspiration observations with windy tropical conditions

NASA Astrophysics Data System (ADS)

Anderson, R. G.; Wang, D.; Tirado-Corbalá, R.; Zhang, H.; Ayars, J. E.

2014-06-01

Standardized reference evapotranspiration (ET) and ecosystem-specific vegetation coefficients are frequently used to estimate actual ET. However, equations for calculating reference ET have not been well validated in tropical environments. We measured ET (ETEC) using Eddy Covariance (EC) towers at two irrigated sugarcane fields on the leeward (dry) side of Maui, Hawaii, USA in contrasting climates. We calculated reference ET at the fields using the short (ET0) and tall (ETr) vegetation versions of the American Society for Civil Engineers (ASCE) equation. The ASCE equations were compared to the Priestley-Taylor ET (ETPT) and ETEC. Reference ET from the ASCE approaches exceeded ETEC during the mid-period (when vegetation coefficients suggest ETEC should exceed reference ET). At the windier tower site, cumulative ETr exceeded ETEC by 854 mm over the course of the mid-period (267 days). At the less windy site, mid-period ETr still exceeded ETEC, but the difference was smaller (443 mm). At both sites, ETPT approximated mid-period ETEC more closely than the ASCE equations ((ETPT-ETEC) < 170 mm). Analysis of applied water and precipitation, soil moisture, leaf stomatal resistance, and canopy cover suggest that the lower observed ETEC was not the result of water stress or reduced vegetation cover. Use of a custom calibrated bulk canopy resistance improved the reference ET estimate and reduced seasonal ET discrepancy relative to ETPT and ETEC for the less windy field and had mixed performance at the windier field. These divergences suggest that modifications to reference ET equations may be warranted in some tropical regions.

Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum).

PubMed

Chaouachi, Maher; El Malki, Redouane; Berard, Aurélie; Romaniuk, Marcel; Laval, Valérie; Brunel, Dominique; Bertheau, Yves

2008-03-26

The labeling of products containing genetically modified organisms (GMO) is linked to their quantification since a threshold for the presence of fortuitous GMOs in food has been established. This threshold is calculated from a combination of two absolute quantification values: one for the specific GMO target and the second for an endogenous reference gene specific to the taxon. Thus, the development of reliable methods to quantify GMOs using endogenous reference genes in complex matrixes such as food and feed is needed. Plant identification can be difficult in the case of closely related taxa, which moreover are subject to introgression events. Based on the homology of beta-fructosidase sequences obtained from public databases, two couples of consensus primers were designed for the detection, quantification, and differentiation of four Solanaceae: potato (Solanum tuberosum), tomato (Solanum lycopersicum), pepper (Capsicum annuum), and eggplant (Solanum melongena). Sequence variability was studied first using lines and cultivars (intraspecies sequence variability), then using taxa involved in gene introgressions, and finally, using taxonomically close taxa (interspecies sequence variability). This study allowed us to design four highly specific TaqMan-MGB probes. A duplex real time PCR assay was developed for simultaneous quantification of tomato and potato. For eggplant and pepper, only simplex real time PCR tests were developed. The results demonstrated the high specificity and sensitivity of the assays. We therefore conclude that beta-fructosidase can be used as an endogenous reference gene for GMO analysis.

Rapid quantitative detection of glucose content in glucose injection by reaction headspace gas chromatography.

PubMed

Xie, Wei-Qi; Gong, Yi-Xian; Yu, Kong-Xian

2017-10-20

This work investigates an automated technique for rapid detecting the glucose content in glucose injection by reaction headspace gas chromatography (HS-GC). This method is based on the oxidation reaction of glucose in glucose injection with potassium dichromate. The carbon dioxide (CO 2 ) formed from the oxidation reaction can be quantitatively detected by GC. The results show that the relative standard deviation (RSD) of the present method was within 2.91%, and the measured glucose contents in glucose injection closely match those quantified by the reference method (relative differences <6.45%). The new HS-GC technique is rapid, practical and can be used to the batch detection of the glucose content in glucose injection related applications. Copyright © 2017 Elsevier B.V. All rights reserved.

A de novo redesign of the WW domain

PubMed Central

Kraemer-Pecore, Christina M.; Lecomte, Juliette T.J.; Desjarlais, John R.

2003-01-01

We have used a sequence prediction algorithm and a novel sampling method to design protein sequences for the WW domain, a small β-sheet motif. The procedure, referred to as SPANS, designs sequences to be compatible with an ensemble of closely related polypeptide backbones, mimicking the inherent flexibility of proteins. Two designed sequences (termed SPANS-WW1 and SPANS-WW2), using only naturally occurring l-amino acids, were selected for study and the corresponding polypeptides were prepared in Escherichia coli. Circular dichroism data suggested that both purified polypeptides adopted secondary structure features related to those of the target without the aid of disulfide bridges or bound cofactors. The structure exhibited by SPANS-WW2 melted cooperatively by raising the temperature of the solution. Further analysis of this polypeptide by proton nuclear magnetic resonance spectroscopy demonstrated that at 5°C, it folds into a structure closely resembling a natural WW domain. This achievement constitutes one of a small number of successful de novo protein designs through fully automated computational methods and highlights the feasibility of including backbone flexibility in the design strategy. PMID:14500877

A de novo redesign of the WW domain.

PubMed

Kraemer-Pecore, Christina M; Lecomte, Juliette T J; Desjarlais, John R

2003-10-01

We have used a sequence prediction algorithm and a novel sampling method to design protein sequences for the WW domain, a small beta-sheet motif. The procedure, referred to as SPANS, designs sequences to be compatible with an ensemble of closely related polypeptide backbones, mimicking the inherent flexibility of proteins. Two designed sequences (termed SPANS-WW1 and SPANS-WW2), using only naturally occurring L-amino acids, were selected for study and the corresponding polypeptides were prepared in Escherichia coli. Circular dichroism data suggested that both purified polypeptides adopted secondary structure features related to those of the target without the aid of disulfide bridges or bound cofactors. The structure exhibited by SPANS-WW2 melted cooperatively by raising the temperature of the solution. Further analysis of this polypeptide by proton nuclear magnetic resonance spectroscopy demonstrated that at 5 degrees C, it folds into a structure closely resembling a natural WW domain. This achievement constitutes one of a small number of successful de novo protein designs through fully automated computational methods and highlights the feasibility of including backbone flexibility in the design strategy.

Semantic and visual determinants of face recognition in a prosopagnosic patient.

PubMed

Dixon, M J; Bub, D N; Arguin, M

1998-05-01

Prosopagnosia is the neuropathological inability to recognize familiar people by their faces. It can occur in isolation or can coincide with recognition deficits for other nonface objects. Often, patients whose prosopagnosia is accompanied by object recognition difficulties have more trouble identifying certain categories of objects relative to others. In previous research, we demonstrated that objects that shared multiple visual features and were semantically close posed severe recognition difficulties for a patient with temporal lobe damage. We now demonstrate that this patient's face recognition is constrained by these same parameters. The prosopagnosic patient ELM had difficulties pairing faces to names when the faces shared visual features and the names were semantically related (e.g., Tonya Harding, Nancy Kerrigan, and Josee Chouinard -three ice skaters). He made tenfold fewer errors when the exact same faces were associated with semantically unrelated people (e.g., singer Celine Dion, actress Betty Grable, and First Lady Hillary Clinton). We conclude that prosopagnosia and co-occurring category-specific recognition problems both stem from difficulties disambiguating the stored representations of objects that share multiple visual features and refer to semantically close identities or concepts.

Correlation Analysis of PM10 and the Incidence of Lung Cancer in Nanchang, China.

PubMed

Zhou, Yi; Li, Lianshui; Hu, Lei

2017-10-19

Air pollution and lung cancer are closely related. In 2013, the World Health Organization listed outdoor air pollution as carcinogenic and regarded it as the most widespread carcinogen that humans are currently exposed to. Here, grey correlation and data envelopment analysis methods are used to determine the pollution factors causing lung cancer among residents in Nanchang, China, and identify population segments which are more susceptible to air pollution. This study shows that particulate matter with particle sizes below 10 micron (PM 10 ) is most closely related to the incidence of lung cancer among air pollution factors including annual mean concentrations of SO₂, NO₂, PM 10 , annual haze days, and annual mean Air Pollution Index/Air Quality Index (API/AQI). Air pollution has a greater impact on urban inhabitants as compared to rural inhabitants. When gender differences are considered, women are more likely to develop lung cancer due to air pollution. Smokers are more likely to suffer from lung cancer. These results provide a reference for the government to formulate policies to reduce air pollutant emissions and strengthen anti-smoking measures.

Correlation Analysis of PM10 and the Incidence of Lung Cancer in Nanchang, China

PubMed Central

Zhou, Yi; Li, Lianshui; Hu, Lei

2017-01-01

Air pollution and lung cancer are closely related. In 2013, the World Health Organization listed outdoor air pollution as carcinogenic and regarded it as the most widespread carcinogen that humans are currently exposed to. Here, grey correlation and data envelopment analysis methods are used to determine the pollution factors causing lung cancer among residents in Nanchang, China, and identify population segments which are more susceptible to air pollution. This study shows that particulate matter with particle sizes below 10 micron (PM10) is most closely related to the incidence of lung cancer among air pollution factors including annual mean concentrations of SO2, NO2, PM10, annual haze days, and annual mean Air Pollution Index/Air Quality Index (API/AQI). Air pollution has a greater impact on urban inhabitants as compared to rural inhabitants. When gender differences are considered, women are more likely to develop lung cancer due to air pollution. Smokers are more likely to suffer from lung cancer. These results provide a reference for the government to formulate policies to reduce air pollutant emissions and strengthen anti-smoking measures. PMID:29048397

Closing the Reference Interview: Implications for Policy and Practice.

ERIC Educational Resources Information Center

Nolan, Christopher W.

1992-01-01

Discusses reasons why patrons or librarians terminate the reference interview, including the content of the interview, interpersonal dynamics, and institutional or policy factors. Goals and objectives of the person terminating the interview are considered, and guidelines for policy development and performance improvement are offered. (30…

40 CFR 65.140 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... FEDERAL AIR RULE Closed Vent Systems, Control Devices, and Routing to a Fuel Gas System or a Process § 65..., shutdown, and malfunction provisions in § 65.6) apply to routing emissions to processes, fuel gas systems, closed vent systems, control devices, and recovery devices where another subpart expressly references the...

75 FR 68330 - Closed Meeting of the Threat Reduction Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-05

... DEPARTMENT OF DEFENSE Office of the Secretary Closed Meeting of the Threat Reduction Advisory... advisory committee meeting of the Threat Reduction Advisory Committee (Hereafter referred to as ``the...: Contact Mr. Eric Wright, Defense Threat Reduction Agency/AST, 8725 John J. Kingman Road, MS 6201, Fort...

Coupling GIS and multivariate approaches to reference site selection for wadeable stream monitoring.

PubMed

Collier, Kevin J; Haigh, Andy; Kelly, Johlene

2007-04-01

Geographic Information System (GIS) was used to identify potential reference sites for wadeable stream monitoring, and multivariate analyses were applied to test whether invertebrate communities reflected a priori spatial and stream type classifications. We identified potential reference sites in segments with unmodified vegetation cover adjacent to the stream and in >85% of the upstream catchment. We then used various landcover, amenity and environmental impact databases to eliminate sites that had potential anthropogenic influences upstream and that fell into a range of access classes. Each site identified by this process was coded by four dominant stream classes and seven zones, and 119 candidate sites were randomly selected for follow-up assessment. This process yielded 16 sites conforming to reference site criteria using a conditional-probabilistic design, and these were augmented by an additional 14 existing or special interest reference sites. Non-metric multidimensional scaling (NMS) analysis of percent abundance invertebrate data indicated significant differences in community composition among some of the zones and stream classes identified a priori providing qualified support for this framework in reference site selection. NMS analysis of a range standardised condition and diversity metrics derived from the invertebrate data indicated a core set of 26 closely related sites, and four outliers that were considered atypical of reference site conditions and subsequently dropped from the network. Use of GIS linked to stream typology, available spatial databases and aerial photography greatly enhanced the objectivity and efficiency of reference site selection. The multi-metric ordination approach reduced variability among stream types and bias associated with non-random site selection, and provided an effective way to identify representative reference sites.

Reference as an Interactive Achievement: Sequential and Longitudinal Analyses of Labeling Interactions in Shared Book Reading and Free Play

PubMed Central

Heller, Vivien; Rohlfing, Katharina J.

2017-01-01

The present study examines how young children and their caregivers establish reference by jointly developing stable patterns of bodily, perceptual, and interactive coordination. Our longitudinal investigation focuses on two mother–child dyads engaged in picture-book reading and play. The dyads were videotaped at home once every 6 weeks while the children aged from 9 to 24 months. Inspired by conversation analysis and multimodal analysis, our developmental approach builds on the insight that the situated and embodied production of reference is fundamentally an interactive achievement. To examine the acquisition of reference, we developed a descriptive instrument that takes account of not only the dyad's joint accomplishment but also each participant's contributions to it. The instrument is based on the sequential reconstruction of the jobs that both participants have to accomplish jointly in order to achieve reference: establishing visual perception as a relevant resource, constituting a domain of scrutiny, locating a target, and construing the (meaning of the) referent. Methodologically, these jobs serve as a tertium comparationis for the longitudinal comparison of both the adult's as well as the child's contributions to establishing reference. We used this instrument to examine (1) what bodily and verbal resources the participants employed, and (2) how their contributions to accomplishing the jobs changed over time. Findings showed that the acquisition of reference was closely related to the child's increasing ability to recognize, fulfill, and set up conditional relevancies. We conclude that the adult's dynamic and contextualized use of conditional relevancies, recipient design, and observability is a crucial driving force in the acquisition of reference. PMID:28261122

Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting.

PubMed

Romi, Wahengbam; Keisam, Santosh; Ahmed, Giasuddin; Jeyaram, Kumaraswamy

2014-02-28

Meyerozyma guilliermondii (anamorph Candida guilliermondii) and Meyerozyma caribbica (anamorph Candida fermentati) are closely related species of the genetically heterogenous M. guilliermondii complex. Conventional phenotypic methods frequently misidentify the species within this complex and also with other species of the Saccharomycotina CTG clade. Even the long-established sequencing of large subunit (LSU) rRNA gene remains ambiguous. We also faced similar problem during identification of yeast isolates of M. guilliermondii complex from indigenous bamboo shoot fermentation in North East India. There is a need for development of reliable and accurate identification methods for these closely related species because of their increasing importance as emerging infectious yeasts and associated biotechnological attributes. We targeted the highly variable internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) and identified seven restriction enzymes through in silico analysis for differentiating M. guilliermondii from M. caribbica. Fifty five isolates of M. guilliermondii complex which could not be delineated into species-specific taxonomic ranks by API 20 C AUX and LSU rRNA gene D1/D2 sequencing were subjected to ITS-restriction fragment length polymorphism (ITS-RFLP) analysis. TaqI ITS-RFLP distinctly differentiated the isolates into M. guilliermondii (47 isolates) and M. caribbica (08 isolates) with reproducible species-specific patterns similar to the in silico prediction. The reliability of this method was validated by ITS1-5.8S-ITS2 sequencing, mitochondrial DNA RFLP and electrophoretic karyotyping. We herein described a reliable ITS-RFLP method for distinct differentiation of frequently misidentified M. guilliermondii from M. caribbica. Even though in silico analysis differentiated other closely related species of M. guilliermondii complex from the above two species, it is yet to be confirmed by in vitro analysis using reference strains. This method can be used as a reliable tool for rapid and accurate identification of closely related species of M. guilliermondii complex and for differentiating emerging infectious yeasts of the Saccharomycotina CTG clade.

Memory for Details with Self-Referencing

PubMed Central

Serbun, Sarah J.; Shih, Joanne Y.; Gutchess, Angela H.

2011-01-01

Self-referencing benefits item memory, but little is known about the ways in which referencing the self affects memory for details. Experiment 1 assessed whether the effects of self-referencing operate only at the item, or general, level or also enhance memory for specific visual details of objects. Participants incidentally encoded objects by making judgments in reference to the self, a close other (one’s mother), or a familiar other (Bill Clinton). Results indicate that referencing the self or a close other enhances both specific and general memory. Experiments 2 and 3 assessed verbal memory for source in a task that relied on distinguishing between different mental operations (internal sources). Results indicate that self-referencing disproportionately enhances source memory, relative to conditions referencing other people, semantic, or perceptual information. We conclude that self-referencing not only enhances specific memory for both visual and verbal information, but can disproportionately improve memory for specific internal source details as well. PMID:22092106

Speckle interferometry using fiber optic phase stepping

NASA Technical Reports Server (NTRS)

Mercer, Carolyn R.; Beheim, Glenn

1989-01-01

A system employing closed-loop phase-stepping is used to measure the out-of-plane deformation of a diffusely reflecting object. Optical fibers are used to provide reference and object beam illumination for a standard two-beam speckle interferometer, providing set-up flexibility and ease of alignment. Piezoelectric fiber-stretchers and a phase-measurement/servo system are used to provide highly accurate phase steps. Intensity data is captured with a charge-injection-device camera, and is converted into a phase map using a desktop computer. The closed-loop phase-stepping system provides 90 deg phase steps which are accurate to 0.02 deg, greatly improving this system relative to open-loop interferometers. The system is demonstrated on a speckle interferometer, measuring the rigid-body translation of a diffusely reflecting object with an accuracy + or - 10 deg, or roughly + or - 15 nanometers. This accuracy is achieved without the use of a pneumatically mounted optics table.

Continuity, change and complexity in the performance of masculinity among elite young footballers in England.

PubMed

Roberts, Steven; Anderson, Eric; Magrath, Rory

2017-06-01

Following recent research evidencing that young men are redefining the essential components of what it is to be a man, this paper draws on qualitative interviews with 22 elite-level, English Premier League academy level football (soccer) players to investigate their performances and understandings of masculinity in relation to decreasing homohysteria. Even in this gender-segregated, near-total institution, these working-class, non-educationally aspiring adolescents evidence an attenuated performance of 'maleness' and improved attitudinal disposition toward homosexuality. Congruent with insights developed by inclusive masculinity scholars, respondents maintained emotional closeness and physical tactility with male teammates and friends. These more inclusive attitudes and homosocial behaviours were, however, slightly more conservative than in other recent research. We close by explaining this variation with reference to theoretical apparatus' provided by Goffman and Bourdieu to advance theoretical debates about social class and masculinities. © London School of Economics and Political Science 2016.

Memory for details with self-referencing.

PubMed

Serbun, Sarah J; Shih, Joanne Y; Gutchess, Angela H

2011-11-01

Self-referencing benefits item memory, but little is known about the ways in which referencing the self affects memory for details. Experiment 1 assessed whether the effects of self-referencing operate only at the item, or general, level or whether they also enhance memory for specific visual details of objects. Participants incidentally encoded objects by making judgements in reference to the self, a close other (one's mother), or a familiar other (Bill Clinton). Results indicate that referencing the self or a close other enhances both specific and general memory. Experiments 2 and 3 assessed verbal memory for source in a task that relied on distinguishing between different mental operations (internal sources). The results indicate that self-referencing disproportionately enhances source memory, relative to conditions referencing other people, semantic, or perceptual information. We conclude that self-referencing not only enhances specific memory for both visual and verbal information, but can also disproportionately improve memory for specific internal source details.

Longitudinal Relationships Among Perceived Injunctive and Descriptive Norms and Marijuana Use

PubMed Central

Napper, Lucy E.; Kenney, Shannon R.; Hummer, Justin F.; Fiorot, Sara; LaBrie, Joseph W.

2016-01-01

Objective: The current study uses longitudinal data to examine the relative influence of perceived descriptive and injunctive norms for proximal and distal referents on marijuana use. Method: Participants were 740 undergraduate students (67% female) who completed web-based surveys at two time points 12 months apart. Time 1 measures included reports of marijuana use, approval, perceived descriptive norms, and perceived injunctive norms for the typical student, close friends, and parents. At Time 2, students reported on their marijuana use. Results: Results of a path analysis suggest that, after we controlled for Time 1 marijuana use, greater perceived friend approval indirectly predicted Time 2 marijuana use as mediated by personal approval. Greater perceived parental approval was both indirectly and directly associated with greater marijuana use at follow-up. Perceived typical-student descriptive norms were neither directly nor indirectly related to Time 2 marijuana use. Conclusions: The findings support the role of proximal injunctive norms in predicting college student marijuana use up to 12 months later. The results indicate the potential importance of developing normative interventions that incorporate the social influences of proximal referents. PMID:27172578

Gene expression in caged fish as indicators of contaminants exposure in tropical karstic water bodies.

PubMed

Rodríguez-Fuentes, Gabriela; Luna-Ramírez, Karen S; Soto, Mélina; Richardson, Kristine L

2012-04-01

Karstic areas in Yucatan are very permeable, which allows contaminants to move rapidly into the aquifer. In the present study, we evaluated gene expression of vitellogenin (VTG) and cytochrome P-450 1A (CYP1A) in caged juvenile zebrafish deployed for 15 days in 13 different water bodies, cenotes and aguadas, throughout karstic region of the Yucatan peninsula. Gene expression was evaluated using qRT-PCR. Results indicated induction of VTG in 7 water bodies with respect to reference cage. The highest relative VTG expression, about 3000 times higher than reference cage, was found in an aguada close to a cattle farm. CYP1A induction with respect to reference cage was observed in 3 water bodies, all of them located near villages or used for tourist activities. Pollutants and biomarkers of effect should be monitored in these water bodies in order to have a better understanding of the actual levels of pollutants that are present at Yucatan's aquifer and the potential risk to human and environmental health. Copyright © 2011 Elsevier Ltd. All rights reserved.

DROMO Propagator Revisited

NASA Astrophysics Data System (ADS)

Urrutxua, H.; Sanjurjo-Rivo, M.; Peláez, J.

2013-12-01

In year 2000 a house-made orbital propagator was developed by the SDGUPM (former Grupo de Dinámica de Tethers) based in a set of redundant variables including Euler parameters. This propagator was called DROMO. and it was mainly used in numerical simulations of electrodynamic tethers. It was presented for the first time in the international meeting V Jornadas de Trabajo en Mecánica Celeste, held in Albarracín, Spain, in 2002 (see reference 1). The special perturbation method associated with DROMO can be consulted in the paper.2 In year 1975, Andre Deprit in reference 3 proposes a propagation scheme very similar to the one in which DROMO is based, by using the ideal frame concept of Hansen. The different approaches used in references 3 and 2 gave rise to a small controversy. In this paper we carried out a different deduction of the DROMO propagator, underlining its close relation with the Hansen ideal frame concept, and also the similarities and the differences with the theory carried out by Deprit in 3. Simultaneously we introduce some improvements in the formulation that leads to a more synthetic propagator.

ADHD and math - The differential effect on calculation and estimation.

PubMed

Ganor-Stern, Dana; Steinhorn, Ofir

2018-05-31

Adults with ADHD were compared to controls when solving multiplication problems exactly and when estimating the results of multidigit multiplication problems relative to reference numbers. The ADHD participants were slower than controls in the exact calculation and in the estimation tasks, but not less accurate. The ADHD participants were similar to controls in showing enhanced accuracy and speed for smaller problem sizes, for trials in which the reference numbers were smaller (vs. larger) than the exact answers and for reference numbers that were far (vs. close) from the exact answer. The two groups similarly used the approximated calculation and the sense of magnitude strategies. They differed however in strategy execution, mainly of the approximated calculation strategy, which requires working memory resources. The increase in reaction time associated with using the approximated calculation strategy was larger for the ADHD compared to the control participants. Thus, ADHD seems to selectively impair calculation processes in estimation tasks that rely on working memory, but it does not hamper estimation skills that are based on sense of magnitude. The educational implications of these findings are discussed. Copyright © 2018. Published by Elsevier B.V.

John Dewey and Robert Pirsig: An Invitation to "Fresh Seeing."

ERIC Educational Resources Information Center

Granger, David A.

While reading John Dewey's "Art as Experience" and Robert Pirsig's "Zen and the Art of Motorcycle Maintenance: An Inquiry into Values," a graduate student observed close affinities between what Dewey referred to as "experience" and Pirsig referred to as "quality." Both texts are concerned with cultivating…

Nutrient and chlorophyll relations in selected streams of the New England Coastal Basins in Massachusetts and New Hampshire, June-September 2001

USGS Publications Warehouse

Riskin, Melissa L.; Deacon, J.R.; Liebman, M.L.; Robinson, K.W.

2003-01-01

The U.S. Environmental Protection Agency is developing guidance to assist states with defining nutrient criteria for rivers and streams and to better describe nutrient-algal relations. As part of this effort, 13 wadeable stream sites were selected, primarily in eastern Massachusetts, for a nutrient-assessment study during the summer of 2001. The sites represent a range of water-quality impairment conditions (reference, moderately impaired, impaired) based on state regulatory agency assessments and previously assessed nitrogen, phosphorus, and dissolved-oxygen data. In addition, a combination of open- and closed-canopy locations were sampled at six of the sites to investigate the effect of sunlight on algal growth. Samples for nutrients and for chlorophyll I from phytoplankton and periphyton were collected at all stream sites. Total nitrogen (dissolved nitrite + nitrate + total ammonia + organic nitrogen) and total phosphorus (phosphorus in an unfiltered water sample) concentrations were lowest at reference sites and highest at impaired sites. There were statistically significant differences (p < 0.05) among reference, moderately impaired, and impaired sites for total nitrogen and total phosphorus. Chlorophyll a concentrations from phytoplankton were not significantly different among site impairment designations. Concentrations of chlorophyll a from periphyton were highest at nutrient-impaired open-canopy sites. Chlorophyll a concentrations from periphyton samples were positively correlated with total nitrogen and total phosphorus at the open- and closed-canopy sites. Correlations were higher at open-canopy sites (p < 0.05, rho = 0.64 to 0.71) than at closed-canopy sites (p < 0.05, rho = 0.36 to 0.40). Statistically significant differences in the median concentrations of chlorophyll a from periphyton samples were observed between the open- and closed-canopy sites (p < 0.05). Total nitrogen and total phosphorus data from moderately impaired and impaired sites in this study exceeded the preliminary U.S. Environmental Protection Agency nutrient criteria values for the coastal region of New England. In an effort to establish more appropriate nutrient and chlorophyll criteria for streams in the New England coastal region, relations between total nitrogen and total phosphorus to periphyton chlorophyll a in wadeable streams from this study were quantified to present potential techniques for determining nutrient concentrations. Linear regression was used to estimate the total nitrogen and total phosphorus concentrations that corresponded to various chlorophyll a concentrations. On the basis of this relation, a median concentration for moderately enriched streams of 21 milligrams per square meter (mg/m2) of periphyton chlorophyll a from the literature corresponded to estimated concentrations of 1.3 milligrams per liter (mg/L) for total nitrogen and 0.12 mg/L for total phosphorus. The median concentration for periphyton chlorophyll a from the literature is similar to the 50th-percentile concentration of periphyton chlorophyll a (17 mg/m2) calculated with the data from open-canopy sites in this study. The 25th-percentile concentration for periphyton chlorophyll a of all open-canopy sites (5.2 mg/m2) and the 75th-percentile concentration for periphyton chlorophyll a of open-canopy reference sites (16 mg/m2) also were plotted to provide additional estimates and methods for developing total nitrogen and total phosphorus criteria. The 25th-percentile concentrations of total nitrogen and total phosphorus were calculated based on all sites in this study and were used as another potential criteria estimation. A concentration of 0.64 mg/L for total nitrogen and 0.030 mg/L for total phosphorus were calculated. As another possible method to develop threshold concentrations, the 10th-percentile concentrations of total nitrogen and total phosphorus were calculated based on all the impaired sites in this study. A concentration threshold of 0

Self-regulation and the problem of human autonomy: does psychology need choice, self-determination, and will?

PubMed

Ryan, Richard M; Deci, Edward L

2006-12-01

The term autonomy literally refers to regulation by the self. Its opposite, heteronomy, refers to controlled regulation, or regulation that occurs without self-endorsement. At a time when philosophers and economists are increasingly detailing the nature of autonomy and recognizing its social and practical significance, many psychologists are questioning the reality and import of autonomy and closely related phenomena such as will, choice, and freedom. Using the framework of self-determination theory (Ryan & Deci, 2000), we review research concerning the benefits of autonomous versus controlled regulation for goal performance, persistence, affective experience, quality of relationships, and well-being across domains and cultures. We also address some of the controversies and terminological issues surrounding the construct of autonomy, including critiques of autonomy by biological reductionists, cultural relativists, and behaviorists. We conclude that there is a universal and cross-developmental value to autonomous regulation when the construct is understood in an exacting way.

Calmodulin Polymerase Chain Reaction–Restriction Fragment Length Polymorphism for Leishmania Identification and Typing

PubMed Central

Miranda, Aracelis; Samudio, Franklyn; González, Kadir; Saldaña, Azael; Brandão, Adeilton; Calzada, Jose E.

2016-01-01

A precise identification of Leishmania species involved in human infections has epidemiological and clinical importance. Herein, we describe a preliminary validation of a restriction fragment length polymorphism assay, based on the calmodulin intergenic spacer region, as a tool for detecting and typing Leishmania species. After calmodulin amplification, the enzyme HaeIII yielded a clear distinction between reference strains of Leishmania mexicana, Leishmania amazonensis, Leishmania infantum, Leishmania lainsoni, and the rest of the Viannia reference species analyzed. The closely related Viannia species: Leishmania braziliensis, Leishmania panamensis, and Leishmania guyanensis, are separated in a subsequent digestion step with different restriction enzymes. We have developed a more accessible molecular protocol for Leishmania identification/typing based on the exploitation of part of the calmodulin gene. This methodology has the potential to become an additional tool for Leishmania species characterization and taxonomy. PMID:27352873

GeneImp: Fast Imputation to Large Reference Panels Using Genotype Likelihoods from Ultralow Coverage Sequencing

PubMed Central

Spiliopoulou, Athina; Colombo, Marco; Orchard, Peter; Agakov, Felix; McKeigue, Paul

2017-01-01

We address the task of genotype imputation to a dense reference panel given genotype likelihoods computed from ultralow coverage sequencing as inputs. In this setting, the data have a high-level of missingness or uncertainty, and are thus more amenable to a probabilistic representation. Most existing imputation algorithms are not well suited for this situation, as they rely on prephasing for computational efficiency, and, without definite genotype calls, the prephasing task becomes computationally expensive. We describe GeneImp, a program for genotype imputation that does not require prephasing and is computationally tractable for whole-genome imputation. GeneImp does not explicitly model recombination, instead it capitalizes on the existence of large reference panels—comprising thousands of reference haplotypes—and assumes that the reference haplotypes can adequately represent the target haplotypes over short regions unaltered. We validate GeneImp based on data from ultralow coverage sequencing (0.5×), and compare its performance to the most recent version of BEAGLE that can perform this task. We show that GeneImp achieves imputation quality very close to that of BEAGLE, using one to two orders of magnitude less time, without an increase in memory complexity. Therefore, GeneImp is the first practical choice for whole-genome imputation to a dense reference panel when prephasing cannot be applied, for instance, in datasets produced via ultralow coverage sequencing. A related future application for GeneImp is whole-genome imputation based on the off-target reads from deep whole-exome sequencing. PMID:28348060

Comparative genomics analysis of Streptococcus agalactiae reveals that isolates from cultured tilapia in China are closely related to the human strain A909.

PubMed

Liu, Guangjin; Zhang, Wei; Lu, Chengping

2013-11-11

Streptococcus agalactiae, also referred to as Group B Streptococcus (GBS), is a frequent resident of the rectovaginal tract in humans, and a major cause of neonatal infection. In addition, S. agalactiae is a known fish pathogen, which compromises food safety and represents a zoonotic hazard. The complete genome sequence of the piscine S. agalactiae isolate GD201008-001 was compared with 14 other piscine, human and bovine strains to explore their virulence determinants, evolutionary relationships and the genetic basis of host tropism in S. agalactiae. The pan-genome of S. agalactiae is open and its size increases with the addition of newly sequenced genomes. The core genes shared by all isolates account for 50 ~ 70% of any single genome. The Chinese piscine isolates GD201008-001 and ZQ0910 are phylogenetically distinct from the Latin American piscine isolates SA20-06 and STIR-CD-17, but are closely related to the human strain A909, in the context of the clustered regularly interspaced short palindromic repeats (CRISPRs), prophage, virulence-associated genes and phylogenetic relationships. We identified a unique 10 kb gene locus in Chinese piscine strains. Isolates from cultured tilapia in China have a close genomic relationship with the human strain A909. Our findings provide insight into the pathogenesis and host-associated genome content of piscine S. agalactiae isolated in China.

Skin complaints in buildings with indoor climate problems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stenberg, B.

1989-01-01

The Sick Building Syndrome (SBS), as defined by the World Health Organization (WHO), is a combination of both common and unspecific symptoms. Few studies have been published with detailed descriptions of clinical findings. One of the few dermatological references with a close relation to sick buildings is the so-called low humidity occupational dermatoses. Since 1982, an increasing number of outpatients from building with indoor climate problems have been investigated at the Department of Dermatology in Umea, Sweden. The most common findings regarding work-related diseases have been seborrheic dermatitis, facial erythema, periorbital eczema, rosacea, urticaria, and itching folliculitis. It is suggestedmore » that physical, chemical, and psychological factors are of importance in producing these symptoms.« less

A reference system for animal biometrics: application to the northern leopard frog

USGS Publications Warehouse

Petrovska-Delacretaz, D.; Edwards, A.; Chiasson, J.; Chollet, G.; Pilliod, D.S.

2014-01-01

Reference systems and public databases are available for human biometrics, but to our knowledge nothing is available for animal biometrics. This is surprising because animals are not required to give their agreement to be in a database. This paper proposes a reference system and database for the northern leopard frog (Lithobates pipiens). Both are available for reproducible experiments. Results of both open set and closed set experiments are given.

Wound-induced suberization genes are differentially expressed during closing layer and wound periderm formation

USDA-ARS?s Scientific Manuscript database

Tuber wounds incurred at harvest and upon seed cutting require rapid suberization as a major part of the healing process to prevent infection and desiccation. The first stage of this healing process is referred to as closing layer development and is followed by the second stage, wound periderm deve...

The influence of ergonomic factors and perceptual-motor abilities on handwriting performance.

PubMed

Tseng, M H; Cermak, S A

1993-10-01

Difficulty with handwriting is one of the most frequent reasons that children in the public schools are referred to occupational therapy. Current research on the influence of ergonomic factors, such as pencil grip and pressure, and perceptual-motor factors traditionally believed to affect handwriting, is reviewed. Factors such as visual perception show little relationship to handwriting, whereas tactile-kinesthetic, visual-motor, and motor planning appear to be more closely related to handwriting. By better understanding the ergonomic and perceptual-motor factors that contribute to and influence handwriting, therapists will be better able to design rationally based intervention programs.

Effect of morning bright light on body temperature, plasma cortisol and wrist motility measured during 24 hour of constant conditions.

PubMed

Foret, J; Aguirre, A; Touitou, Y; Clodoré, M; Benoit, O

1993-06-11

Using 24 h constant conditions, time course of body temperature, plasma cortisol and wrist motility was measured in response to a 3 day morning 2 h bright light pulse. This protocol demonstrated that a 2000 lux illumination was sufficient to elicit a shift of about 2 h of temperature minimum and cortisol peak. In reference session, actimetric recordings showed a circadian time course, closely in relation with core temperature. Bright light pulse resulted in a decrease of amplitude and a disappearance of circadian pattern of actimetry.

The taxonomic status of "Halobacterium marismortui" from the Dead Sea: a comparison with Halobacterium vallismortis

NASA Technical Reports Server (NTRS)

Oren, A.; Lau, P. P.; Fox, G. E.

1988-01-01

A Halobacterium strain, isolated by Ginzburg et al. from the Dead Sea in the late 1960's, often referred to as "Halobacterium marismortui" or "Halobacterium of the Dead Sea" (deposited in the American Type Culture Collection as ATCC 43049) was compared with Halobacterium (Haloarcula) vallismortis ATCC 29715. The strains appeared to be very closely related, as shown by the near identity of their 5S and 16S ribosomal RNA's, and a large number of other common properties. Distinct differences exist, however, in cell morphology, and in their potency to utilize different sugars and other compounds.

Genome of Drosophila suzukii, the Spotted Wing Drosophila

PubMed Central

Chiu, Joanna C.; Jiang, Xuanting; Zhao, Li; Hamm, Christopher A.; Cridland, Julie M.; Saelao, Perot; Hamby, Kelly A.; Lee, Ernest K.; Kwok, Rosanna S.; Zhang, Guojie; Zalom, Frank G.; Walton, Vaughn M.; Begun, David J.

2013-01-01

Drosophila suzukii Matsumura (spotted wing drosophila) has recently become a serious pest of a wide variety of fruit crops in the United States as well as in Europe, leading to substantial yearly crop losses. To enable basic and applied research of this important pest, we sequenced the D. suzukii genome to obtain a high-quality reference sequence. Here, we discuss the basic properties of the genome and transcriptome and describe patterns of genome evolution in D. suzukii and its close relatives. Our analyses and genome annotations are presented in a web portal, SpottedWingFlyBase, to facilitate public access. PMID:24142924

Photovoltaic and thermal energy conversion for solar powered satellites

NASA Technical Reports Server (NTRS)

Von Tiesenhausen, G. F.

1976-01-01

A summary is provided concerning the most important aspects of present investigations related to a use of solar power satellites (SPS) as a future source of terrestrial energy. General SPS characteristics are briefly considered, early work is reviewed, and a description of current investigations is presented. System options presently under study include a photovoltaic array, a thermionic system, and a closed Brayton cycle. Attention is given to system reference options, basic building blocks, questions of system analysis and engineering, photovoltaic conversion, and the utility interface. It is concluded that an SPS may be cost effective compared to terrestrial systems by 1995.

Multimedia Information Networks in Social Media

NASA Astrophysics Data System (ADS)

Cao, Liangliang; Qi, Guojun; Tsai, Shen-Fu; Tsai, Min-Hsuan; Pozo, Andrey Del; Huang, Thomas S.; Zhang, Xuemei; Lim, Suk Hwan

The popularity of personal digital cameras and online photo/video sharing community has lead to an explosion of multimedia information. Unlike traditional multimedia data, many new multimedia datasets are organized in a structural way, incorporating rich information such as semantic ontology, social interaction, community media, geographical maps, in addition to the multimedia contents by themselves. Studies of such structured multimedia data have resulted in a new research area, which is referred to as Multimedia Information Networks. Multimedia information networks are closely related to social networks, but especially focus on understanding the topics and semantics of the multimedia files in the context of network structure. This chapter reviews different categories of recent systems related to multimedia information networks, summarizes the popular inference methods used in recent works, and discusses the applications related to multimedia information networks. We also discuss a wide range of topics including public datasets, related industrial systems, and potential future research directions in this field.

Soil Respiration in European Grasslands in Relation to Climate and Assimilate Supply

PubMed Central

Bahn, Michael; Rodeghiero, Mirco; Anderson-Dunn, Margaret; Dore, Sabina; Gimeno, Cristina; Drösler, Matthias; Williams, Michael; Ammann, Christof; Berninger, Frank; Flechard, Chris; Jones, Stephanie; Balzarolo, Manuela; Kumar, Suresh; Newesely, Christian; Priwitzer, Tibor; Raschi, Antonio; Siegwolf, Rolf; Susiluoto, Sanna; Tenhunen, John; Wohlfahrt, Georg; Cernusca, Alexander

2010-01-01

Soil respiration constitutes the second largest flux of carbon (C) between terrestrial ecosystems and the atmosphere. This study provides a synthesis of soil respiration (Rs) in 20 European grasslands across a climatic transect, including ten meadows, eight pastures and two unmanaged grasslands. Maximum rates of Rs (Rsmax), Rs at a reference soil temperature (10°C; Rs10) and annual Rs (estimated for 13 sites) ranged from 1.9 to 15.9 μmol CO2 m−2 s−1, 0.3 to 5.5 μmol CO2 m−2 s−1 and 58 to 1988 g C m−2 y−1, respectively. Values obtained for Central European mountain meadows are amongst the highest so far reported for any type of ecosystem. Across all sites Rsmax was closely related to Rs10. Assimilate supply affected Rs at timescales from daily (but not necessarily diurnal) to annual. Reductions of assimilate supply by removal of aboveground biomass through grazing and cutting resulted in a rapid and a significant decrease of Rs. Temperature-independent seasonal fluctuations of Rs of an intensively managed pasture were closely related to changes in leaf area index (LAI). Across sites Rs10 increased with mean annual soil temperature (MAT), LAI and gross primary productivity (GPP), indicating that assimilate supply overrides potential acclimation to prevailing temperatures. Also annual Rs was closely related to LAI and GPP. Because the latter two parameters were coupled to MAT, temperature was a suitable surrogate for deriving estimates of annual Rs across the grasslands studied. These findings contribute to our understanding of regional patterns of soil C fluxes and highlight the importance of assimilate supply for soil CO2 emissions at various timescales. PMID:20936099

Choice of Reference Serum Creatinine in Defining AKI

PubMed Central

Siew, Edward D.; Matheny, Michael E.

2015-01-01

Background/Aims The study of acute kidney injury (AKI) has expanded with the increasing availability of electronic health records and the use of standardized definitions. Understanding the impact of AKI between settings is limited by heterogeneity in the selection of reference creatinine to anchor the definition of AKI. In this mini-review, we discuss different approaches used to select reference creatinine and their relative merits and limitations. Methods We reviewed the literature to obtain representative examples of published baseline creatinine definitions when pre-hospital data were not available, as well as literature evaluating estimation of baseline renal function, using Pubmed and reference back-tracing within known works. Results 1) Prehospital creatinine values are useful in determining reference creatinine, and in high-risk populations, the mean outpatient serum creatinine value 7-365 days before hospitalization closely approximates nephrology adjudication, 2) in patients without pre-hospital data, the eGFR 75 approach does not reliably estimate true AKI incidence in most at-risk populations 3) using the lowest inpatient serum creatinine may be reasonable, especially in those with preserved kidney function, but may generously estimate AKI incidence and severity and miss community-acquired AKI that does not fully resolve, 4) using more specific definitions of AKI (e.g. KIDGO Stage 2 and 3) may help to reduce the effects of misclassification when using surrogate values, and 5) leveraging available clinical data may help refine the estimate of reference creatinine. Conclusions Choosing reference creatinine for AKI calculation is important for AKI classification and study interpretation. We recommend obtaining data on pre-hospital kidney function, wherever possible. In studies where surrogate estimates are used, transparency in how they are applied and discussion that informs the reader of potential biases should be provided. Further work to refine the estimation of reference creatinine is needed. PMID:26332325

Children's Picture Interpretation: Appearance or Intention?

ERIC Educational Resources Information Center

Armitage, Emma; Allen, Melissa L.

2015-01-01

Pictures are defined by their creator's intentions and resemblance to their real world referents. Here we examine whether young children follow a realist route (e.g., focusing on how closely pictures resemble their referents) or intentional route (e.g., focusing on what a picture is intended to represent by its artist) when identifying a picture's…

30 CFR 203.74 - When will MMS reconsider its determination?

Code of Federal Regulations, 2011 CFR

2011-07-01

... Sulfur General Royalty Relief for Pre-Act Deep Water Leases and for Development and Expansion Projects... as calculated under this paragraph. (1) Your current reference price is a weighted-average of daily... calendar months; (2) Your base reference price is a weighted average of daily closing prices on the NYMEX...

Time-efficient high-resolution whole-brain three-dimensional macromolecular proton fraction mapping

PubMed Central

Yarnykh, Vasily L.

2015-01-01

Purpose Macromolecular proton fraction (MPF) mapping is a quantitative MRI method that reconstructs parametric maps of a relative amount of macromolecular protons causing the magnetization transfer (MT) effect and provides a biomarker of myelination in neural tissues. This study aimed to develop a high-resolution whole-brain MPF mapping technique utilizing a minimal possible number of source images for scan time reduction. Methods The described technique is based on replacement of an actually acquired reference image without MT saturation by a synthetic one reconstructed from R1 and proton density maps, thus requiring only three source images. This approach enabled whole-brain three-dimensional MPF mapping with isotropic 1.25×1.25×1.25 mm3 voxel size and scan time of 20 minutes. The synthetic reference method was validated against standard MPF mapping with acquired reference images based on data from 8 healthy subjects. Results Mean MPF values in segmented white and gray matter appeared in close agreement with no significant bias and small within-subject coefficients of variation (<2%). High-resolution MPF maps demonstrated sharp white-gray matter contrast and clear visualization of anatomical details including gray matter structures with high iron content. Conclusions Synthetic reference method improves resolution of MPF mapping and combines accurate MPF measurements with unique neuroanatomical contrast features. PMID:26102097

Efficient and accurate local single reference correlation methods for high-spin open-shell molecules using pair natural orbitals

NASA Astrophysics Data System (ADS)

Hansen, Andreas; Liakos, Dimitrios G.; Neese, Frank

2011-12-01

A production level implementation of the high-spin open-shell (spin unrestricted) single reference coupled pair, quadratic configuration interaction and coupled cluster methods with up to doubly excited determinants in the framework of the local pair natural orbital (LPNO) concept is reported. This work is an extension of the closed-shell LPNO methods developed earlier [F. Neese, F. Wennmohs, and A. Hansen, J. Chem. Phys. 130, 114108 (2009), 10.1063/1.3086717; F. Neese, A. Hansen, and D. G. Liakos, J. Chem. Phys. 131, 064103 (2009), 10.1063/1.3173827]. The internal space is spanned by localized orbitals, while the external space for each electron pair is represented by a truncated PNO expansion. The laborious integral transformation associated with the large number of PNOs becomes feasible through the extensive use of density fitting (resolution of the identity (RI)) techniques. Technical complications arising for the open-shell case and the use of quasi-restricted orbitals for the construction of the reference determinant are discussed in detail. As in the closed-shell case, only three cutoff parameters control the average number of PNOs per electron pair, the size of the significant pair list, and the number of contributing auxiliary basis functions per PNO. The chosen threshold default values ensure robustness and the results of the parent canonical methods are reproduced to high accuracy. Comprehensive numerical tests on absolute and relative energies as well as timings consistently show that the outstanding performance of the LPNO methods carries over to the open-shell case with minor modifications. Finally, hyperfine couplings calculated with the variational LPNO-CEPA/1 method, for which a well-defined expectation value type density exists, indicate the great potential of the LPNO approach for the efficient calculation of molecular properties.

Orthostatic intolerance and postural tachycardia syndrome as suspected adverse effects of vaccination against human papilloma virus.

PubMed

Brinth, Louise S; Pors, Kirsten; Theibel, Ann C; Mehlsen, Jesper

2015-05-21

Infections with human papilloma virus (HPV) can result in cervical, oropharyngeal, anal, and penile cancer and vaccination programs have been launched in many countries as a preventive measure. We report the characteristics of a number of patients with a syndrome of orthostatic intolerance, headache, fatigue, cognitive dysfunction, and neuropathic pain starting in close relation to HPV vaccination. Patients were referred for orthostatic intolerance following HPV vaccination. Symptoms of autonomic dysfunction were quantified by standardised questionnaire. The diagnosis of postural orthostatic tachycardia syndrome (POTS) rested on finding a sustained heart rate increment of >30 min(-1) (>40 min(-1) in adolescents) or to levels >120 min(-1) during orthostatic challenge. 35 women aged 23.3 ± 7.1 years participated. Twenty-five had a high level of physical activity before vaccination and irregular periods were reported by all patients not on treatment with oral contraception. Serum bilirubin was below the lower detection limit in 17 patients. Twenty-one of the referred patients fulfilled the criteria for a diagnosis of POTS (60%, 95%CI 43-77%). All patients had orthostatic intolerance, 94% nausea, 82% chronic headache, 82% fatigue, 77% cognitive dysfunction, 72% segmental dystonia, 68% neuropathic pain. In a population referred for symptoms of orthostatic intolerance and other symptoms consistent with autonomic dysfunction that began in close temporal association with a quadrivalent HPV vaccination, we identified a 60% prevalence of POTS. Further work is urgently needed to elucidate the potential for a causal link between the vaccine and circulatory abnormalities and to establish targeted treatment options for the affected patients. Copyright © 2015 Elsevier Ltd. All rights reserved.

Solving Math Problems Approximately: A Developmental Perspective

PubMed Central

Ganor-Stern, Dana

2016-01-01

Although solving arithmetic problems approximately is an important skill in everyday life, little is known about the development of this skill. Past research has shown that when children are asked to solve multi-digit multiplication problems approximately, they provide estimates that are often very far from the exact answer. This is unfortunate as computation estimation is needed in many circumstances in daily life. The present study examined 4th graders, 6th graders and adults’ ability to estimate the results of arithmetic problems relative to a reference number. A developmental pattern was observed in accuracy, speed and strategy use. With age there was a general increase in speed, and an increase in accuracy mainly for trials in which the reference number was close to the exact answer. The children tended to use the sense of magnitude strategy, which does not involve any calculation but relies mainly on an intuitive coarse sense of magnitude, while the adults used the approximated calculation strategy which involves rounding and multiplication procedures, and relies to a greater extent on calculation skills and working memory resources. Importantly, the children were less accurate than the adults, but were well above chance level. In all age groups performance was enhanced when the reference number was smaller (vs. larger) than the exact answer and when it was far (vs. close) from it, suggesting the involvement of an approximate number system. The results suggest the existence of an intuitive sense of magnitude for the results of arithmetic problems that might help children and even adults with difficulties in math. The present findings are discussed in the context of past research reporting poor estimation skills among children, and the conditions that might allow using children estimation skills in an effective manner. PMID:27171224

Achromatic self-referencing interferometer

DOEpatents

Feldman, M.

1994-04-19

A self-referencing Mach-Zehnder interferometer is described for accurately measuring laser wavefronts over a broad wavelength range (for example, 600 nm to 900 nm). The apparatus directs a reference portion of an input beam to a reference arm and a measurement portion of the input beam to a measurement arm, recombines the output beams from the reference and measurement arms, and registers the resulting interference pattern ([open quotes]first[close quotes] interferogram) at a first detector. Optionally, subportions of the measurement portion are diverted to second and third detectors, which respectively register intensity and interferogram signals which can be processed to reduce the first interferogram's sensitivity to input noise. The reference arm includes a spatial filter producing a high quality spherical beam from the reference portion, a tilted wedge plate compensating for off-axis aberrations in the spatial filter output, and mirror collimating the radiation transmitted through the tilted wedge plate. The apparatus includes a thermally and mechanically stable baseplate which supports all reference arm optics, or at least the spatial filter, tilted wedge plate, and the collimator. The tilted wedge plate is mounted adjustably with respect to the spatial filter and collimator, so that it can be maintained in an orientation in which it does not introduce significant wave front errors into the beam propagating through the reference arm. The apparatus is polarization insensitive and has an equal path length configuration enabling measurement of radiation from broadband as well as closely spaced laser line sources. 3 figures.

Compensating effect of sap velocity for stand density leads to uniform hillslope-scale forest transpiration across a steep valley cross-section

NASA Astrophysics Data System (ADS)

Renner, Maik; Hassler, Sibylle; Blume, Theresa; Weiler, Markus; Hildebrandt, Anke; Guderle, Marcus; Schymanski, Stan; Kleidon, Axel

2016-04-01

Roberts (1983) found that forest transpiration is relatively uniform across different climatic conditions and suggested that forest transpiration is a conservative process compensating for environmental heterogeneity. Here we test this hypothesis at a steep valley cross-section composed of European Beech in the Attert basin in Luxemburg. We use sapflow, soil moisture, biometric and meteorological data from 6 sites along a transect to estimate site scale transpiration rates. Despite opposing hillslope orientation, different slope angles and forest stand structures, we estimated relatively similar transpiration responses to atmospheric demand and seasonal transpiration totals. This similarity is related to a negative correlation between sap velocity and site-average sapwood area. At the south facing sites with an old, even-aged stand structure and closed canopy layer, we observe significantly lower sap velocities but similar stand-average transpiration rates compared to the north-facing sites with open canopy structure, tall dominant trees and dense understorey. This suggests that plant hydraulic co-ordination allows for flexible responses to environmental conditions leading to similar transpiration rates close to the water and energy limits despite the apparent heterogeneity in exposition, stand density and soil moisture. References Roberts, J. (1983). Forest transpiration: A conservative hydrological process? Journal of Hydrology 66, 133-141.

Empirical Selection of Informative Microsatellite Markers within Co-ancestry Pig Populations Is Required for Improving the Individual Assignment Efficiency

PubMed Central

Li, Y. H.; Chu, H. P.; Jiang, Y. N.; Lin, C. Y.; Li, S. H.; Li, K. T.; Weng, G. J.; Cheng, C. C.; Lu, D. J.; Ju, Y. T.

2014-01-01

The Lanyu is a miniature pig breed indigenous to Lanyu Island, Taiwan. It is distantly related to Asian and European pig breeds. It has been inbred to generate two breeds and crossed with Landrace and Duroc to produce two hybrids for laboratory use. Selecting sets of informative genetic markers to track the genetic qualities of laboratory animals and stud stock is an important function of genetic databases. For more than two decades, Lanyu derived breeds of common ancestry and crossbreeds have been used to examine the effectiveness of genetic marker selection and optimal approaches for individual assignment. In this paper, these pigs and the following breeds: Berkshire, Duroc, Landrace and Yorkshire, Meishan and Taoyuan, TLRI Black Pig No. 1, and Kaohsiung Animal Propagation Station Black pig are studied to build a genetic reference database. Nineteen microsatellite markers (loci) provide information on genetic variation and differentiation among studied breeds. High differentiation index (FST) and Cavalli-Sforza chord distances give genetic differentiation among breeds, including Lanyu’s inbred populations. Inbreeding values (FIS) show that Lanyu and its derived inbred breeds have significant loss of heterozygosity. Individual assignment testing of 352 animals was done with different numbers of microsatellite markers in this study. The testing assigned 99% of the animals successfully into their correct reference populations based on 9 to 14 markers ranking D-scores, allelic number, expected heterozygosity (HE) or FST, respectively. All miss-assigned individuals came from close lineage Lanyu breeds. To improve individual assignment among close lineage breeds, microsatellite markers selected from Lanyu populations with high polymorphic, heterozygosity, FST and D-scores were used. Only 6 to 8 markers ranking HE, FST or allelic number were required to obtain 99% assignment accuracy. This result suggests empirical examination of assignment-error rates is required if discernible levels of co-ancestry exist. In the reference group, optimum assignment accuracy was achievable achieved through a combination of different markers by ranking the heterozygosity, FST and allelic number of close lineage populations. PMID:25049996

A Preliminary Research Plan for Development of a Photosynthetic Link in a Closed Ecological Life Support System

NASA Technical Reports Server (NTRS)

Morgan, P. W.

1979-01-01

The use of higher plants in a closed ecological life support system for long duration space missions involving large numbers of people is considered. The approach to planning and developing both the habitat for a long term space mission and closed ecological life support systems are discussed with emphasis on environmental compatibility and integrated systems design. The requirements of photosynthetic processes are summarized and evaluated in terms of their availability within a closed ecological life support environment. Specific references are recommended as a data base for future research on this topic.

Heat Transfer Analysis of a Closed Brayton Cycle Space Radiator

NASA Technical Reports Server (NTRS)

Juhasz, Albert J.

2007-01-01

This paper presents a mathematical analysis of the heat transfer processes taking place in a radiator for a closed cycle gas turbine (CCGT), also referred to as a Closed Brayton Cycle (CBC) space power system. The resulting equations and relationships have been incorporated into a radiator sub-routine of a numerical triple objective CCGT optimization program to determine operating conditions yielding maximum cycle efficiency, minimum radiator area and minimum overall systems mass. Study results should be of interest to numerical modeling of closed cycle Brayton space power systems and to the design of fluid cooled radiators in general.

Decomposition of the linking number of a closed ribbon: A problem from molecular biology

PubMed Central

Fuller, F. Brock

1978-01-01

A closed duplex DNA molecule relaxed and containing nucleosomes has a different linking number from the same molecule relaxed and without nucleosomes. What does this say about the structure of the nucleosome? A mathematical study of this question is made, representing the DNA molecule by a ribbon. It is shown that the linking number of a closed ribbon can be decomposed into the linking number of a reference ribbon plus a sum of locally determined “linking differences.” PMID:16592550

The Reference Genome of the Halophytic Plant Eutrema salsugineum

PubMed Central

Yang, Ruolin; Jarvis, David E.; Chen, Hao; Beilstein, Mark A.; Grimwood, Jane; Jenkins, Jerry; Shu, ShengQiang; Prochnik, Simon; Xin, Mingming; Ma, Chuang; Schmutz, Jeremy; Wing, Rod A.; Mitchell-Olds, Thomas; Schumaker, Karen S.; Wang, Xiangfeng

2013-01-01

Halophytes are plants that can naturally tolerate high concentrations of salt in the soil, and their tolerance to salt stress may occur through various evolutionary and molecular mechanisms. Eutrema salsugineum is a halophytic species in the Brassicaceae that can naturally tolerate multiple types of abiotic stresses that typically limit crop productivity, including extreme salinity and cold. It has been widely used as a laboratorial model for stress biology research in plants. Here, we present the reference genome sequence (241 Mb) of E. salsugineum at 8× coverage sequenced using the traditional Sanger sequencing-based approach with comparison to its close relative Arabidopsis thaliana. The E. salsugineum genome contains 26,531 protein-coding genes and 51.4% of its genome is composed of repetitive sequences that mostly reside in pericentromeric regions. Comparative analyses of the genome structures, protein-coding genes, microRNAs, stress-related pathways, and estimated translation efficiency of proteins between E. salsugineum and A. thaliana suggest that halophyte adaptation to environmental stresses may occur via a global network adjustment of multiple regulatory mechanisms. The E. salsugineum genome provides a resource to identify naturally occurring genetic alterations contributing to the adaptation of halophytic plants to salinity and that might be bioengineered in related crop species. PMID:23518688

Comparative genomics approach to detecting split-coding regions in a low-coverage genome: lessons from the chimaera Callorhinchus milii (Holocephali, Chondrichthyes).

PubMed

Dessimoz, Christophe; Zoller, Stefan; Manousaki, Tereza; Qiu, Huan; Meyer, Axel; Kuraku, Shigehiro

2011-09-01

Recent development of deep sequencing technologies has facilitated de novo genome sequencing projects, now conducted even by individual laboratories. However, this will yield more and more genome sequences that are not well assembled, and will hinder thorough annotation when no closely related reference genome is available. One of the challenging issues is the identification of protein-coding sequences split into multiple unassembled genomic segments, which can confound orthology assignment and various laboratory experiments requiring the identification of individual genes. In this study, using the genome of a cartilaginous fish, Callorhinchus milii, as test case, we performed gene prediction using a model specifically trained for this genome. We implemented an algorithm, designated ESPRIT, to identify possible linkages between multiple protein-coding portions derived from a single genomic locus split into multiple unassembled genomic segments. We developed a validation framework based on an artificially fragmented human genome, improvements between early and recent mouse genome assemblies, comparison with experimentally validated sequences from GenBank, and phylogenetic analyses. Our strategy provided insights into practical solutions for efficient annotation of only partially sequenced (low-coverage) genomes. To our knowledge, our study is the first formulation of a method to link unassembled genomic segments based on proteomes of relatively distantly related species as references.

Comparative genomics approach to detecting split-coding regions in a low-coverage genome: lessons from the chimaera Callorhinchus milii (Holocephali, Chondrichthyes)

PubMed Central

Zoller, Stefan; Manousaki, Tereza; Qiu, Huan; Meyer, Axel; Kuraku, Shigehiro

2011-01-01

Recent development of deep sequencing technologies has facilitated de novo genome sequencing projects, now conducted even by individual laboratories. However, this will yield more and more genome sequences that are not well assembled, and will hinder thorough annotation when no closely related reference genome is available. One of the challenging issues is the identification of protein-coding sequences split into multiple unassembled genomic segments, which can confound orthology assignment and various laboratory experiments requiring the identification of individual genes. In this study, using the genome of a cartilaginous fish, Callorhinchus milii, as test case, we performed gene prediction using a model specifically trained for this genome. We implemented an algorithm, designated ESPRIT, to identify possible linkages between multiple protein-coding portions derived from a single genomic locus split into multiple unassembled genomic segments. We developed a validation framework based on an artificially fragmented human genome, improvements between early and recent mouse genome assemblies, comparison with experimentally validated sequences from GenBank, and phylogenetic analyses. Our strategy provided insights into practical solutions for efficient annotation of only partially sequenced (low-coverage) genomes. To our knowledge, our study is the first formulation of a method to link unassembled genomic segments based on proteomes of relatively distantly related species as references. PMID:21712341

Developing a taxonomy of science concepts based on a scale of empirical distance

NASA Astrophysics Data System (ADS)

Jones, Brian L.

1990-01-01

The term ‘concept’ is used in different ways within educational literature and has at least two different, although related, referents in relation to science knowledge, namely, public knowledge and private understandings. A taxonomic structure for ‘science concepts’ (public knowledge) has been developed to provide a rationale for the choice of phenomena to be used in the investigation of students’ ‘concepts’ and also to act as a frame of reference for generating insights about the data to be collected. Furthermore, it may be a useful heuristic to predict other science concepts likely to be highly problematic in school teaching situations and thus worthy of detailed research. The taxonomy, called a ‘Scale of Empirical Distance’ (SED), enables science concepts to be mapped according to their degree of closeness to concrete realities. The scale shows a recognition of the empirical basis of science concepts and the role of human senses in the perception of the material world even though “absolute objectivity of observation is not a possible ideal of science” as Harre (1972) has noted. The scale uses two binary variables, namely, ‘visual’ and ‘tactile’, to generate four categories of science concepts ranging on a continuum from concrete to abstract. Some concepts related to ‘matter’ will be classified and discussed.

Roads, railways, and childhood cancers

PubMed Central

Knox, E G

2006-01-01

Study objectives To locate geographical sources of engine exhaust emissions in Great Britain and to link them with the birth addresses of children dying from cancer. To estimate the cancer initiating roles of nearby roads and railways and to measure effective ranges. Design Birth and death addresses of all children born between 1955 and 1980 in Great Britain, and dying from leukaemia or other cancer during those years, were linked to locations of railway stations, bus stations, ferry terminals, railways, roads, canals, and rivers. Nearest distances to births and deaths were measured, and migration data relating to children who had moved house were analysed. Excesses of close to hazard birth addresses, compared with close to hazard death addresses, indicate a high prenatal or early postnatal risk of cancer initiation. Setting and subjects Child cancer birth and death addresses and their map references were extracted from an earlier inquiry. Map references of putative hazards were downloaded from the Ordnance Survey national digital map of Great Britain. These data are recorded to a precision of one metre and have ground accuracies around 20 metres. Main results Significant birth excesses were found within short distances of bus stations, railway stations, ferries, railways, and A,B class roads, with a relative risk of 2.1 within 100 m, tapering to neutral after 3.0 km. About 24% of child cancers were attributable to these joint birth proximities. Roads exerted the major effect. Conclusions Child cancer initiations are strongly determined by prenatal or early postnatal exposures to engine exhaust gases, probably through maternal inhalation and accumulation of carcinogens over many months. The main active substance is probably 1,3‐butadiene. PMID:16415262

Distribution of the ACME-arcA gene among methicillin-resistant Staphylococcus aureus from England and Wales.

PubMed

Ellington, Matthew J; Yearwood, Lianne; Ganner, Mark; East, Claire; Kearns, Angela M

2008-01-01

The ST8-SCCmecIVa (USA300) methicillin-resistant Staphylococcus aureus (MRSA) clone can harbour the arginine catabolic mobile element (ACME). The arc gene cluster within the ACME may function as a virulence or strain survival factor. We determined the distribution of the ACME-associated arcA gene among genetically diverse MRSA from around England and Wales. MRSA isolates (n = 203) of diverse genetic types, referred to the England and Wales Staphylococcus reference laboratory, were tested for the presence of the ACME-arcA gene. ACME-arcA-positive isolates were characterized by toxin gene profiling, PFGE and spa sequence typing. MICs of a range of antimicrobials were also determined. The ACME-arcA gene was detected in 17 isolates. Twelve were related to known ST8-MRSA-SCCmecIVa isolates of the USA300 lineage by pulsotype and were resistant to oxacillin, with variable ciprofloxacin and erythromycin resistance. Outside the USA300 lineage, four of the remaining five ACME-arcA isolates were closely related ST97-MRSA-SCCmecV, Panton-Valentine leucocidin (PVL)-negative, resistant to oxacillin and variously resistant to erythromycin, ciprofloxacin, clindamycin, gentamicin, tetracycline and fusidic acid. The remaining isolate was ST1, PVL-positive and resistant to fusidic acid as well as oxacillin. Thirteen out of the 17 isolates were associated with skin and soft tissue infections. The detection of ACME-arcA in diverse MRSA types highlights the mobility of the elements encoding ACME-arcA genes. The diversity of strain types and resistance profiles among ACME-arcA-encoding MRSA is a cause for public-health concern and demands continued surveillance and close monitoring.

Juggling with Indianness in the Gestation of Translation with Special Reference to the English Translation of a Hindi Story

ERIC Educational Resources Information Center

Priya, K.

2014-01-01

This paper is an attempt to look closely at the process of translating dramas with special reference to the Hindi story Aadmi ka Baccha ("The Child of a Man") by Yashpal in India and the role and significance of prose transcreations in today's changing global scenario.

26 CFR 1.338-2 - Nomenclature and definitions; mechanics of the section 338 election.

Code of Federal Regulations, 2010 CFR

2010-04-01

... purchase of stock under section 338(a)(2), a reference to new target with respect to its own deemed... target is the common parent on the acquisition date. (17) Target; old target; new target. Target is the... before the close of target's acquisition date. New target refers to target for subsequent periods. (18...

26 CFR 1.338-2 - Nomenclature and definitions; mechanics of the section 338 election.

Code of Federal Regulations, 2011 CFR

2011-04-01

... purchase of stock under section 338(a)(2), a reference to new target with respect to its own deemed... target is the common parent on the acquisition date. (17) Target; old target; new target. Target is the... before the close of target's acquisition date. New target refers to target for subsequent periods. (18...

Standard and reference materials for marine science. Third edition. Technical memo

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cantillo, A.Y.

1992-08-01

The third edition of the catalog of reference materials suited for use in marine science, originally compiled in 1986 for NOAA, IOC, and UNEP. The catalog lists close to 2,000 reference materials from sixteen producers and contains information about their proper use, sources, availability, and analyte concentrations. Indices are included for elements, isotopes, and organic compounds, as are cross references to CAS registry numbers, alternate names, and chemical structures of selected organic compounds. The catalog is being published independently by both NOAA and IOC/UNEP and is available from NOAA/NOS/ORCA in electronic form.

College students' responses to mental health status updates on Facebook.

PubMed

Egan, Katie G; Koff, Rosalind N; Moreno, Megan A

2013-01-01

Facebook is widely used by the college population, and previous research has shown that mental health references on Facebook are common. Focus groups of college students were held to determine their views of mental health references seen in their peers' Facebook profiles. Students' views of mental health references varied from being serious calls for help, to being jokes or attention-seeking behavior. Responses to mental health references depended on the participants' offline relationship with the poster. Students would contact close friends through a phone call or in-person conversation, but would not approach acquaintances. The prevalence of mental health references on Facebook, and the awareness of these references by college students, may present opportunities for future peer intervention efforts.

Assessing performance of closed-loop insulin delivery systems by continuous glucose monitoring: drawbacks and way forward.

PubMed

Hovorka, Roman; Nodale, Marianna; Haidar, Ahmad; Wilinska, Malgorzata E

2013-01-01

We investigated whether continuous glucose monitoring (CGM) levels can accurately assess glycemic control while directing closed-loop insulin delivery. Data were analyzed retrospectively from 33 subjects with type 1 diabetes who underwent closed-loop and conventional pump therapy on two separate nights. Glycemic control was evaluated by reference plasma glucose and contrasted against three methods based on Navigator (Abbott Diabetes Care, Alameda, CA) CGM levels. Glucose mean and variability were estimated by unmodified CGM levels with acceptable clinical accuracy. Time when glucose was in target range was overestimated by CGM during closed-loop nights (CGM vs. plasma glucose median [interquartile range], 86% [65-97%] vs. 75% [59-91%]; P=0.04) but not during conventional pump therapy (57% [32-72%] vs. 51% [29-68%]; P=0.82) providing comparable treatment effect (mean [SD], 28% [29%] vs. 23% [21%]; P=0.11). Using the CGM measurement error of 15% derived from plasma glucose-CGM pairs (n=4,254), stochastic interpretation of CGM gave unbiased estimate of time in target during both closed-loop (79% [62-86%] vs. 75% [59-91%]; P=0.24) and conventional pump therapy (54% [33-66%] vs. 51% [29-68%]; P=0.44). Treatment effect (23% [24%] vs. 23% [21%]; P=0.96) and time below target were accurately estimated by stochastic CGM. Recalibrating CGM using reference plasma glucose values taken at the start and end of overnight closed-loop was not superior to stochastic CGM. CGM is acceptable to estimate glucose mean and variability, but without adjustment it may overestimate benefit of closed-loop. Stochastic CGM provided unbiased estimate of time when glucose is in target and below target and may be acceptable for assessment of closed-loop in the outpatient setting.

F-16 Low Altitude Navigation and Targeting Infrared System for Night (LANTIRN) and the Night Close Air Support (CAS) Mission

DTIC Science & Technology

1989-06-02

physical reference can be a natural feature, river bend, distinctly shaped wooded area, or lake. Artificial references such as colored smoke or...fluorescent ground panels can also be placed instead of, or as an aid to recognition of the natural feature.ដ> But, artificial references that are effective...Government Printin-gOffice, 1959. Central Intelligence Agenc7. National Intellignece Survey, East Germany, Section 23, Weather and Climate. Washington

Analyzing Forest Inventory Data from Geo-Located Photographs

NASA Astrophysics Data System (ADS)

Toivanen, Timo; Tergujeff, Renne; Andersson, Kaj; Molinier, Matthieu; Häme, Tuomas

2015-04-01

Forests are widely monitored using a variety of remote sensing data and techniques. Remote sensing offers benefits compared to traditional in-situ forest inventories made by experts. One of the main benefits is that the number of ground reference plots can be significantly reduced. Remote sensing of forests can provide reduced costs and time requirement compared to full forest inventories. The availability of ground reference data has been a bottleneck in remote sensing analysis over wide forested areas, as the acquisition of this data is an expensive and slow process. In this paper we present a tool for estimating forest inventory data from geo-located photographs. The tool can be used to estimate in-situ forest inventory data including estimated biomass, tree species, tree height and diameter. The collected in-situ forest measurements can be utilized as a ground reference material for spaceborne or airborne remote sensing data analysis. The GPS based location information with measured forest data makes it possible to introduce measurements easily as in-situ reference data. The central projection geometry of digital photographs allows the use of the relascope principle [1] to measure the basal area of stems per area unit, a variable very closely associated with tree biomass. Relascope is applied all over the world for forest inventory. Experiments with independent ground reference data have shown that in-situ data analysed from photographs can be utilised as reference data for satellite image analysis. The concept was validated by comparing mobile measurements with 54 independent ground reference plots from the Hyytiälä forest research station in Finland [2]. Citizen scientists could provide the manpower for analysing photographs from forests on a global level and support researchers working on tasks related to forests. This low-cost solution can also increase the coverage of forest management plans, particularly in regions where possibilities to invest on expensive planning work are limited. References [1] Bitterlich, W. (1984) The Relascope Idea: Relative Measurements in Forestry, Commonwealth Agricultural Bureaux, Farnham Royal, 1984. [2] Molinier, M., Hame, T., Toivanen, T., Andersson, K., Mutanen, T., Relasphone -- Mobile phone and interactive applications to collect ground reference biomass data for satellite image analysis, Geoscience and Remote Sensing Symposium (IGARSS), 2014 IEEE International, pp. 836-839, 13-18 July 2014, doi: 10.1109/IGARSS.2014.6946554

Bowel obstruction complicated by ischemia: analysis of CT findings.

PubMed

Cox, Veronica L; Tahvildari, Ali M; Johnson, Benjamin; Wei, Wei; Jeffrey, R Brooke

2018-06-01

To analyze CT signs of bowel ischemia in patients with surgical bowel obstruction, and thereby improve CT diagnosis in this common clinical scenario. Surgical and histopathological findings were used as the reference standard. We retrospectively analyzed CT findings in patients brought to surgery for bowel obstruction over 13 years. Etiology of obstruction (adhesion, hernia, etc.) was recorded. Specific CT features of acute mesenteric ischemia (AMI) were analyzed, including bowel wall thickening, mucosal hypoenhancement, and others. 173 cases were eligible for analysis. 21% of cases were positive for bowel ischemia. Volvulus, internal hernia, and closed-loop obstructions showed ischemia rates of 60%, 43%, and 43%; ischemia rate in obstruction from simple adhesion was 21%. Patients with bowel obstruction related to malignancy were never ischemic. Sensitivities and specificities for CT features predicting ischemia were calculated, with wall thickening, hypoenhancement, and pneumatosis showing high specificity for ischemia (86%-100%). Wall thickening, hypoenhancement, and pneumatosis are highly specific CT signs of ischemia in the setting of obstruction. None of the evaluated CT signs were found to be highly sensitive. Overall frequency of ischemia in surgical bowel obstruction is 21%, and 2-3 times that for complex obstructions (volvulus, closed loop, etc.). Obstructions related to malignancy virtually never become ischemic.

Activity concentration and spatial distribution of radionuclides in marine sediments close to the estuary of Shatt al-Arab/Arvand Rud River, the Gulf.

PubMed

Patiris, D L; Tsabaris, C; Anagnostou, C L; Androulakaki, E G; Pappa, F K; Eleftheriou, G; Sgouros, G

2016-06-01

Tigris and Euphrates rivers both emerge in eastern Turkey and cross Syria and Iraq. They unite to Shatt al-Arab/Arvand Rud River and discharge in Arabic/Persian Gulf. The activity concentration of natural and anthropogenic radionuclides was measured during the August of 2011 in a number of surficial sediment samples collected from the seabed along an almost straight line beginning near the estuary mouth and extending seaward. The results exhibited low activity concentration levels and an almost homogeneous spatial distribution except locations where sediment of biogenic origin, poor in radionuclides, dilute their concentrations. Dose rates absorbed by reference marine biota were calculated by the ERICA Assessment Tool considering the contribution of 40 K. The results revealed a relatively low impact of 40 K mainly to species living in, on and close to the seabed. Also, statistical association of radionuclides with selected stable elements (Ca, Ba and Sr) did not indicate presence of by-products related with oil and gas exploitation and transportation activities. Moreover, a semi-empirical sedimentology model applied to reproduce seabed granulometric facies based entirely on radionuclides activity concentrations. Copyright © 2016 Elsevier Ltd. All rights reserved.

Studies of effects of closed microbial ecology. Report of 180-day test period

NASA Technical Reports Server (NTRS)

Kenyon, A. J.

1972-01-01

Experiments were performed to determine the influence closed microbial ecologies have on modification or simplification of natural intestinal flora of ferrets in a closed environmental system. On the basis of previous tests in which certain species (Salmonella and Bacteroides) were decreased at 90 days of enclosure, a second trial was constructed for 180-day tests. In this trial there was little difference in the 8 major classes of intestinal flora between animals in the Open and Closed environmental groups except for the level of Lactobacillus. It is of extreme importance to note that when both Open and Closed groups contracted hemorrhagic gastritis, the interrelationship of this agent with other intestinal flora produced a more profound effect on animals from the Closed Group, particularly with reference to Lactobacillus levels.

The Minkowski metric in non-inertial observer radar coordinates

NASA Astrophysics Data System (ADS)

Minguzzi, E.

2005-12-01

We give a closed expression for the Minkowski (1+1)-dimensional metric in the radar coordinates of an arbitrary non-inertial observer O in terms of O's proper acceleration. Knowledge of the metric allows the non-inertial observer to perform experiments in spacetime without making reference to inertial frames. To clarify the relation between inertial and non-inertial observers the coordinate transformation between radar and inertial coordinates also is given. We show that every conformally flat coordinate system can be regarded as the radar coordinate system of a suitable observer for a suitable parametrization of the observer worldline. Therefore, the coordinate transformation between arbitrarily moving observers is a conformal transformation and conformally invariant (1+1)-dimensional theories lead to the same physics for all observers, independently of their relative motion.

A complete mass spectrometric map for the analysis of the yeast proteome and its application to quantitative trait analysis

PubMed Central

Picotti, Paola; Clement-Ziza, Mathieu; Lam, Henry; Campbell, David S.; Schmidt, Alexander; Deutsch, Eric W.; Röst, Hannes; Sun, Zhi; Rinner, Oliver; Reiter, Lukas; Shen, Qin; Michaelson, Jacob J.; Frei, Andreas; Alberti, Simon; Kusebauch, Ulrike; Wollscheid, Bernd; Moritz, Robert; Beyer, Andreas; Aebersold, Ruedi

2013-01-01

Complete reference maps or datasets, like the genomic map of an organism, are highly beneficial tools for biological and biomedical research. Attempts to generate such reference datasets for a proteome so far failed to reach complete proteome coverage, with saturation apparent at approximately two thirds of the proteomes tested, even for the most thoroughly characterized proteomes. Here, we used a strategy based on high-throughput peptide synthesis and mass spectrometry to generate a close to complete reference map (97% of the genome-predicted proteins) of the S. cerevisiae proteome. We generated two versions of this mass spectrometric map one supporting discovery- (shotgun) and the other hypothesis-driven (targeted) proteomic measurements. The two versions of the map, therefore, constitute a complete set of proteomic assays to support most studies performed with contemporary proteomic technologies. The reference libraries can be browsed via a web-based repository and associated navigation tools. To demonstrate the utility of the reference libraries we applied them to a protein quantitative trait locus (pQTL) analysis, which requires measurement of the same peptides over a large number of samples with high precision. Protein measurements over a set of 78 S. cerevisiae strains revealed a complex relationship between independent genetic loci, impacting on the levels of related proteins. Our results suggest that selective pressure favors the acquisition of sets of polymorphisms that maintain the stoichiometry of protein complexes and pathways. PMID:23334424

First significant image improvement from a sodium-layer laser guide star adaptive optics system at Lick Observatory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olivier, S.S.; Max, C.E.; Friedman, H.W.

1997-07-14

Atmospheric turbulence severely limits the resolution of ground-based telescopes. Adaptive optics can correct for the aberrations caused by the atmosphere, but requires a bright wavefront reference source in close angular proximity to the object being imaged. Since natural reference stars of the necessary brightness are relatively rare, methods of generating artificial reference beacons have been under active investigation for more than a decade. In this paper, we report the first significant image improvement achieved using a sodium-layer laser guide star as a wavefront reference for a high- order adaptive optics system. An artificial beacon was created by resonant scattering frommore » atomic sodium in the mesosphere, at an altitude of 95 km. Using this laser guide star, an adaptive optics system on the 3 m Shane Telescope at Lick Observatory produced a factor of 2.4 increase in peak intensity and a factor of 2 decrease in full width at half maximum of a stellar image, compared with image motion compensation alone. The Strehl ratio when using the laser guide star as the reference was 65% of that obtained with a natural guide star, and the image full widths at half maximum were identical, 0.3 arc sec, using either the laser or the natural guide star. This sodium-layer laser guide star technique holds great promise for the world`s largest telescopes. 24 refs., 4 figs., 1 tab.« less

Program Manager’s Handbook

DTIC Science & Technology

1989-03-01

management business with references for sources of more detailed information on each subject. Recipients are provided with updated and new fact sheets as...at Milestone Force, strategic offense, strategic defense; Army, 0 by the Defense Acquisition .oard An Acquisi- O close combat heavy, close combat...assessment of producibi’,ity: realistic competition. Conce-ts such as teaming, leader - industry surge and mobilization capacity: multiyear follower

[Current problems in determining children's nutritional needs].

PubMed

Koletzko, B; Toschke, A M; Von Kries, R

2004-03-01

Meeting children's nutritional needs is of fundamental importance for their immediate and later health, well-being, and performance. Age-adapted reference values of nutrient intake form the basis for analysis of the current situation and for policy planning, but for many nutrients they cannot be precisely defined due to inadequate scientific data. Therefore, such values are often extrapolated from adult reference values based on age-adapted mean body weight or body surface data, although such extrapolation does not reflect age-related physiological changes. There are considerable differences between various expert recommendations, in part also due to differences in definitions and underlying concepts for deriving reference values. Improvements and international harmonization are urgently needed. Nutritional needs of many children and adolescents are not adequately met at present. A particularly obvious indication is the epidemic-like increase of pediatric overweight and obesity, which could result in markedly increased prevalences of later metabolic syndrome, diabetes, and cardiovascular diseases. Contributing to increasing childhood obesity are low physical activity, changing eating culture and behavior, frequent consumption of high-fat foods with high energy density, and increasing portion sizes. Changes are urgently needed and might be achievable with close collaboration between scientists, public institutions, and industry.

Simple equation for calculation of plasma clearance for evaluation of renal function without urine collection in rats.

PubMed

Liu, Xiang; Peng, Dejun; Tian, Hao; Lu, Chengyu

2017-01-01

To develop an equation for the evaluation of renal function in rats using three dilutions of plasma samples and to validate this method by comparison with a reference method. The investigation was conducted in Sprague-Dawley (SD) rats after delivery of three doses of iohexol, with blood samples collected before and after dosage using a quantitative blood collection method. Plasma iohexol concentrations were detected by high performance liquid chromatography (HPLC). The extraction recovery of iohexol from plasma was >97.30% and the calibration curve was linear (r 2  = 0.9997) over iohexol concentrations ranging from 10 to 1000 µg/mL. The method had an RE of <9.310 and intra- and inter-day RSD of <5.137% and <3.693%, respectively. The plasma clearance values obtained from the equation correlated closely (r = 0.763) with those obtained using the reference method. The relatively correlation in the results obtained using the method under investigation and the reference method indicate that this new equation can be used for preliminary assessment of renal function in rats. © 2016 Asian Pacific Society of Nephrology.

Spatiotemporal signal space separation method for rejecting nearby interference in MEG measurements

NASA Astrophysics Data System (ADS)

Taulu, S.; Simola, J.

2006-04-01

Limitations of traditional magnetoencephalography (MEG) exclude some important patient groups from MEG examinations, such as epilepsy patients with a vagus nerve stimulator, patients with magnetic particles on the head or having magnetic dental materials that cause severe movement-related artefact signals. Conventional interference rejection methods are not able to remove the artefacts originating this close to the MEG sensor array. For example, the reference array method is unable to suppress interference generated by sources closer to the sensors than the reference array, about 20-40 cm. The spatiotemporal signal space separation method proposed in this paper recognizes and removes both external interference and the artefacts produced by these nearby sources, even on the scalp. First, the basic separation into brain-related and external interference signals is accomplished with signal space separation based on sensor geometry and Maxwell's equations only. After this, the artefacts from nearby sources are extracted by a simple statistical analysis in the time domain, and projected out. Practical examples with artificial current dipoles and interference sources as well as data from real patients demonstrate that the method removes the artefacts without altering the field patterns of the brain signals.

The generator coordinate Dirac-Fock method for open-shell atomic systems

NASA Astrophysics Data System (ADS)

Malli, Gulzari L.; Ishikawa, Yasuyuki

1998-11-01

Recently we developed generator coordinate Dirac-Fock and Dirac-Fock-Breit methods for closed-shell systems assuming finite nucleus and have reported Dirac-Fock and Dirac-Fock-Breit energies for the atoms He through Nobelium (Z=102) [see Refs. Reference 10Reference 11Reference 12Reference 13]. In this paper, we generalize our earlier work on closed-shell systems and develop a generator coordinate Dirac-Fock method for open-shell systems. We present results for a number of representative open-shell heavy atoms (with nuclear charge Z>80) including the actinide and superheavy transactinide (with Z>103) atomic systems: Fr (Z=87), Ac (Z=89), and Lr (Z=103) to E113 (eka-thallium, Z=113). The high accuracy obtained in our open-shell Dirac-Fock calculations is similar to that of our closed-shell calculations, and we attribute it to the fact that the representation of the relativistic dynamics of an electron in a spherical ball finite nucleus near the origin in terms of our universal Gaussian basis set is as accurate as that provided by the numerical finite difference method. The DF SCF energies calculated by Desclaux [At. Data. Nucl. Data Tables 12, 311 (1973)] (apart from a typographic error for Fr pointed out here) are higher than those reported here for atoms of some of the superheavy transactinide elements by as much as 5 hartrees (136 eV). We believe that this is due to the use by Desclaux of much larger atomic masses than the currently accepted values for these elements.

Comparative genomics analysis of Streptococcus agalactiae reveals that isolates from cultured tilapia in China are closely related to the human strain A909

PubMed Central

2013-01-01

Background Streptococcus agalactiae, also referred to as Group B Streptococcus (GBS), is a frequent resident of the rectovaginal tract in humans, and a major cause of neonatal infection. In addition, S. agalactiae is a known fish pathogen, which compromises food safety and represents a zoonotic hazard. The complete genome sequence of the piscine S. agalactiae isolate GD201008-001 was compared with 14 other piscine, human and bovine strains to explore their virulence determinants, evolutionary relationships and the genetic basis of host tropism in S. agalactiae. Results The pan-genome of S. agalactiae is open and its size increases with the addition of newly sequenced genomes. The core genes shared by all isolates account for 50 ~ 70% of any single genome. The Chinese piscine isolates GD201008-001 and ZQ0910 are phylogenetically distinct from the Latin American piscine isolates SA20-06 and STIR-CD-17, but are closely related to the human strain A909, in the context of the clustered regularly interspaced short palindromic repeats (CRISPRs), prophage, virulence-associated genes and phylogenetic relationships. We identified a unique 10 kb gene locus in Chinese piscine strains. Conclusions Isolates from cultured tilapia in China have a close genomic relationship with the human strain A909. Our findings provide insight into the pathogenesis and host-associated genome content of piscine S. agalactiae isolated in China. PMID:24215651

Determination of isocyanate groups in the organic intermediates by reaction-based headspace gas chromatography.

PubMed

Xie, Wei-Qi; Chai, Xin-Sheng

2016-10-14

This work reports on a novel method for the determination of isocyanate groups in the related organic intermediates by a reaction-based headspace gas chromatography. The method is based on measuring the CO 2 formed from the reaction between the isocyanate groups in the organic intermediates and water in a closed headspace sample vial at 45°C for 20min. The results showed that the method has a good precision and accuracy, in which the relative standard deviation in the repeatability measurement was 5.26%, and the relative differences between the data obtained by the HS-GC method and the reference back-titration method were within 9.42%. The present method is simple and efficient and is particularly suitable to be used for determining the isocyanate groups in the batch sample analysis. Copyright © 2016 Elsevier B.V. All rights reserved.

Relative deprivation in income and mortality by leading causes among older Japanese men and women: AGES cohort study.

PubMed

Kondo, Naoki; Saito, Masashige; Hikichi, Hiroyuki; Aida, Jun; Ojima, Toshiyuki; Kondo, Katsunori; Kawachi, Ichiro

2015-07-01

Relative deprivation of income is hypothesised to generate frustration and stress through upward social comparison with one's peers. If psychosocial stress is the mechanism, relative deprivation should be more strongly associated with specific health outcomes, such as cardiovascular disease (compared with other health outcomes, eg, non-tobacco-related cancer). We evaluated the association between relative income deprivation and mortality by leading causes, using a cohort of 21 031 community-dwelling adults aged 65 years or older. A baseline mail-in survey was conducted in 2003. Information on cause-specific mortality was obtained from death certificates. Our relative deprivation measure was the Yitzhaki Index, derived from the aggregate income shortfall for each person, relative to individuals with higher incomes in that person's reference group. Reference groups were defined according to gender, age group and same municipality of residence. We identified 1682 deaths during the 4.5 years of follow-up. A Cox regression demonstrated that, after controlling for demographic, health and socioeconomic factors including income, the HR for death from cardiovascular diseases per SD increase in relative deprivation was 1.50 (95% CI 1.09 to 2.08) in men, whereas HRs for mortality by cancer and other diseases were close to the null value. Additional adjustment for depressive symptoms and health behaviours (eg, smoking and preventive care utilisation) attenuated the excess risks for mortality from cardiovascular disease by 9%. Relative deprivation was not associated with mortality for women. The results partially support our hypothesised mechanism: relative deprivation increases health risks via psychosocial stress among men. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Mahan excitons in degenerate wurtzite InN: Photoluminescence spectroscopy and reflectivity measurements

NASA Astrophysics Data System (ADS)

Feneberg, Martin; Däubler, Jürgen; Thonke, Klaus; Sauer, Rolf; Schley, Pascal; Goldhahn, Rüdiger

2008-06-01

Unintentionally degenerately doped n -type hexagonal wurtzite InN samples were studied by using Fourier-transform photoluminescence spectroscopy and reflectivity measurements. We found in luminescence overlapping band acceptor (e,A0) transitions related to two different acceptors with a strong enhancement of their intensities close to the Fermi energy of the electrons recombining with the localized holes. Our explanation is in terms of a Fermi-edge singularity of the electrons due to strongly increased electron-hole scattering. Electron-hole pairs with such resonantly enhanced oscillator strengths have been referred to as Mahan excitons. Temperature-dependent reflectivity measurements confirm this interpretation.

Isolation and characterization of orf viruses from Korean black goats.

PubMed

Oem, Jae-Ku; Chung, Joon-Yee; Kim, Yong-Joo; Lee, Kyoung-Ki; Kim, Seong-Hee; Jung, Byeong-Yeal; Hyun, Bang-Hun

2013-01-01

Five cases of orf virus infection in Korean black goats were diagnosed in our laboratory between 2010 and 2011. One orf virus (ORF/2011) was isolated from an ovine testis cell line (OA3.Ts) for use as a vaccine candidate. Sequences of the major envelope protein and orf virus interferon resistance genes were determined and compared with published reference sequences. Phylogenetic analyses revealed that orf viruses from Korean black goats were most closely related to an isolate (ORF/09/Korea) from dairy goats in Korea. This result indicates that the orf viruses might have been introduced from dairy goats into the Korean black goat population.

The Crystal Structures of Potentially Tautomeric Compounds

NASA Astrophysics Data System (ADS)

Furmanova, Nina G.

1981-08-01

Data on the structures of potentially proto-, metallo-, and carbono-tropic compounds, obtained mainly by X-ray diffraction, are surveyed. The results of neutron and electron diffraction studies have also been partly used. It is shown that a characteristic feature of all the systems considered is the formation of hydrogen or secondary bonds ensuring the contribution of both possible tautomeric forms to the structure. Systematic consideration of the experimental data leads to the conclusion that there is a close relation between the crystal structure and the dynamic behaviour of the molecules in solution and that secondary and hydrogen bonds play a significant role in the tautomeric transition. The bibliography includes 152 references.

IDLN-MSP: Idiolocal normalization of real-time methylation-specific PCR for genetic imbalanced DNA specimens.

PubMed

Santourlidis, Simeon; Ghanjati, Foued; Beermann, Agnes; Hermanns, Thomas; Poyet, Cédric

2016-02-01

Sensitive, accurate, and reliable measurements of tumor cell-specific DNA methylation changes are of fundamental importance in cancer diagnosis, prognosis, and monitoring. Real-time methylation-specific PCR (MSP) using intercalating dyes is an established method of choice for this purpose. Here we present a simple but crucial adaptation of this widely applied method that overcomes a major obstacle: genetic abnormalities in the DNA samples, such as aneuploidy or copy number variations, that could result in inaccurate results due to improper normalization if the copy numbers of the target and reference sequences are not the same. In our idiolocal normalization (IDLN) method, the locus for the normalizing, methylation-independent reference amplification is chosen close to the locus of the methylation-dependent target amplification. This ensures that the copy numbers of both the target and reference sequences will be identical in most cases if they are close enough to each other, resulting in accurate normalization and reliable comparative measurements of DNA methylation in clinical samples when using real-time MSP.

Characterization of system-related geometric distortions in MR images employed in Gamma Knife radiosurgery applications.

PubMed

Pappas, E P; Seimenis, I; Moutsatsos, A; Georgiou, E; Nomikos, P; Karaiskos, P

2016-10-07

This work provides characterization of system-related geometric distortions present in MRIs used in Gamma Knife (GK) stereotactic radiosurgery (SRS) treatment planning. A custom-made phantom, compatible with the Leksell stereotactic frame model G and encompassing 947 control points (CPs), was utilized. MR images were obtained with and without the frame, thus allowing discrimination of frame-induced distortions. In the absence of the frame and following compensation for field inhomogeneities, measured average CP disposition owing to gradient nonlinearities was 0.53 mm. In presence of the frame, contrarily, detected distortion was greatly increased (up to about 5 mm) in the vicinity of the frame base due to eddy currents induced in the closed loop of its aluminum material. Frame-related distortion was obliterated at approximately 90 mm from the frame base. Although the region with the maximum observed distortion may not lie within the GK treatable volume, the presence of the frame results in distortion of the order of 1.5 mm at a 7 cm distance from the center of the Leksell space. Additionally, severe distortions observed outside the treatable volume could possibly impinge on the delivery accuracy mainly by adversely affecting the registration process (e.g. the position of the lower part of the N-shaped fiducials used to define the stereotactic space may be miss-registered). Images acquired with a modified version of the frame developed by replacing its front side with an acrylic bar, thus interrupting the closed aluminum loop and reducing the induced eddy currents, were shown to benefit from relatively reduced distortion. System-related distortion was also identified in patient MR images. Using corresponding CT angiography images as a reference, an offset of 1.1 mm was detected for two vessels lying in close proximity to the frame base, while excellent spatial agreement was observed for a vessel far apart from the frame base.

Characterization of system-related geometric distortions in MR images employed in Gamma Knife radiosurgery applications

NASA Astrophysics Data System (ADS)

Pappas, E. P.; Seimenis, I.; Moutsatsos, A.; Georgiou, E.; Nomikos, P.; Karaiskos, P.

2016-10-01

This work provides characterization of system-related geometric distortions present in MRIs used in Gamma Knife (GK) stereotactic radiosurgery (SRS) treatment planning. A custom-made phantom, compatible with the Leksell stereotactic frame model G and encompassing 947 control points (CPs), was utilized. MR images were obtained with and without the frame, thus allowing discrimination of frame-induced distortions. In the absence of the frame and following compensation for field inhomogeneities, measured average CP disposition owing to gradient nonlinearities was 0.53 mm. In presence of the frame, contrarily, detected distortion was greatly increased (up to about 5 mm) in the vicinity of the frame base due to eddy currents induced in the closed loop of its aluminum material. Frame-related distortion was obliterated at approximately 90 mm from the frame base. Although the region with the maximum observed distortion may not lie within the GK treatable volume, the presence of the frame results in distortion of the order of 1.5 mm at a 7 cm distance from the center of the Leksell space. Additionally, severe distortions observed outside the treatable volume could possibly impinge on the delivery accuracy mainly by adversely affecting the registration process (e.g. the position of the lower part of the N-shaped fiducials used to define the stereotactic space may be miss-registered). Images acquired with a modified version of the frame developed by replacing its front side with an acrylic bar, thus interrupting the closed aluminum loop and reducing the induced eddy currents, were shown to benefit from relatively reduced distortion. System-related distortion was also identified in patient MR images. Using corresponding CT angiography images as a reference, an offset of 1.1 mm was detected for two vessels lying in close proximity to the frame base, while excellent spatial agreement was observed for a vessel far apart from the frame base.

Breast Reference Set Application: GeorgeTuszynski-Temple (2012) — EDRN Public Portal

Cancer.gov

As recommended by the review committee, we will analyze 30 invasive cancer cases and 30 benign controls from the EDRN breast reference set. Cases and controls should be selected that are Caucasian and post-menopausal. It is suggested that the Caucasian cases and controls match as closely as possible in regards to age and body mass index.

Completed Genome Sequences of Strains from 36 Serotypes of Salmonella

PubMed Central

Robertson, James; Yoshida, Catherine; Gurnik, Simone; Rankin, Marisa

2018-01-01

ABSTRACT We report here the completed closed genome sequences of strains representing 36 serotypes of Salmonella. These genome sequences will provide useful references for understanding the genetic variation between serotypes, particularly as references for mapping of raw reads or to create assemblies of higher quality, as well as to aid in studies of comparative genomics of Salmonella. PMID:29348347

Detecting and Correcting Errors in Rapid Aiming Movements: Effects of Movement Time, Distance, and Velocity

ERIC Educational Resources Information Center

Sherwood, David E.

2010-01-01

According to closed-loop accounts of motor control, movement errors are detected by comparing sensory feedback to an acquired reference state. Differences between the reference state and the movement-produced feedback results in an error signal that serves as a basis for a correction. The main question addressed in the current study was how…

Unified Bayesian Estimator of EEG Reference at Infinity: rREST (Regularized Reference Electrode Standardization Technique).

PubMed

Hu, Shiang; Yao, Dezhong; Valdes-Sosa, Pedro A

2018-01-01

The choice of reference for the electroencephalogram (EEG) is a long-lasting unsolved issue resulting in inconsistent usages and endless debates. Currently, both the average reference (AR) and the reference electrode standardization technique (REST) are two primary, apparently irreconcilable contenders. We propose a theoretical framework to resolve this reference issue by formulating both (a) estimation of potentials at infinity, and (b) determination of the reference, as a unified Bayesian linear inverse problem, which can be solved by maximum a posterior estimation. We find that AR and REST are very particular cases of this unified framework: AR results from biophysically non-informative prior; while REST utilizes the prior based on the EEG generative model. To allow for simultaneous denoising and reference estimation, we develop the regularized versions of AR and REST, named rAR and rREST, respectively. Both depend on a regularization parameter that is the noise to signal variance ratio. Traditional and new estimators are evaluated with this framework, by both simulations and analysis of real resting EEGs. Toward this end, we leverage the MRI and EEG data from 89 subjects which participated in the Cuban Human Brain Mapping Project. Generated artificial EEGs-with a known ground truth, show that relative error in estimating the EEG potentials at infinity is lowest for rREST. It also reveals that realistic volume conductor models improve the performances of REST and rREST. Importantly, for practical applications, it is shown that an average lead field gives the results comparable to the individual lead field. Finally, it is shown that the selection of the regularization parameter with Generalized Cross-Validation (GCV) is close to the "oracle" choice based on the ground truth. When evaluated with the real 89 resting state EEGs, rREST consistently yields the lowest GCV. This study provides a novel perspective to the EEG reference problem by means of a unified inverse solution framework. It may allow additional principled theoretical formulations and numerical evaluation of performance.

The Extended-Image Tracking Technique Based on the Maximum Likelihood Estimation

NASA Technical Reports Server (NTRS)

Tsou, Haiping; Yan, Tsun-Yee

2000-01-01

This paper describes an extended-image tracking technique based on the maximum likelihood estimation. The target image is assume to have a known profile covering more than one element of a focal plane detector array. It is assumed that the relative position between the imager and the target is changing with time and the received target image has each of its pixels disturbed by an independent additive white Gaussian noise. When a rotation-invariant movement between imager and target is considered, the maximum likelihood based image tracking technique described in this paper is a closed-loop structure capable of providing iterative update of the movement estimate by calculating the loop feedback signals from a weighted correlation between the currently received target image and the previously estimated reference image in the transform domain. The movement estimate is then used to direct the imager to closely follow the moving target. This image tracking technique has many potential applications, including free-space optical communications and astronomy where accurate and stabilized optical pointing is essential.

Quantum theory of the structure and bonding in proteins. Part 11. A simplified method and its application to the α-amino-isobutyric acid residue

NASA Astrophysics Data System (ADS)

Peters, David; Peters, Jane

Information about the preferred conformation of the α-amino-isobutyric acid residue (α-AIB) is obtained without explicit computation of its wave function. The conformation of lowest energy of this residue is close to the usual helical conformation and so the residue may occur at the 2 position of a type I or I' bend or in either position of a type III or III' bend. The available experimental information refers to a β bend formed from α-AIB-PRO and then the theory and experiment agree that the only possibility is a type III bend. It is predicted that a β sheet structure may be formed at rather higher energy and in the planar and not the pleated form. There is no apparent reason why this residue should not form an α helix. The simplified method used here is closely related to the partitioned potential energy methods which are widely used in this subject.

Parallel conductance estimation by hypertonic dilution method with conductance catheter: effects of the bolus concentration and temperature.

PubMed

Herrera, M C; Olivera, J M; Valentinuzzi, M E

1999-07-01

The conductance catheter has gained momentum since its introduction in cardiovascular dynamics back in 1980. However, measuring errors are still blurring its clinical acceptance. The main objective here was to study the effects of the injected saline concentration and temperature on the evaluation of the parallel conductance, Gp, and thus, on the correction volume Vp. That conductance, Gp, and its associated volume, Vp, were computed using 167 saline dilution curves obtained with boluses at different concentrations and temperatures, injected in seven anesthetized closed-chest dogs. The excursion of the total conductance relative to the steady-state value during a saline maneuver showed good correlation with the injected concentration at both studied temperatures. The reference parallel volume (one reference per dog) was defined as the average value obtained with three successive maneuvers, at 6-M concentration and at body temperature; therefore, the method acted as its own reference. The variation of Vp relative to the reference value was clearly dependent on the injected concentration and on its temperature; dispersion was greater at 22 degrees C than at 40 degrees C. The variability would recognize also other causes, such as uncertainty of the extrapolation procedure and the thoracic redistribution of electrical field lines. As conclusion, it is recommended to characterize each maneuver by its concentration and temperature. Body temperature and 6-M concentration appear as the most recommendable combination for the injectate in most animals. Finally, these results intend to characterize the Vp estimation procedure in order to minimize errors. The variability of Vp, in different experimental conditions, demonstrated that both concentration and temperature are additional parameters that may modify the Gp estimate.

31 CFR 515.339 - Close relative.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Close relative. 515.339 Section 515... § 515.339 Close relative. (a) For purposes of this part, the term close relative used with respect to.... Your mother's first cousin is your close relative for purposes of this part, because you are both no...

Genetics Home Reference: SADDAN

MedlinePlus

... CLOSE navigation Home Page Search Home Health ... delay and acanthosis nigricans) is a rare disorder of bone growth characterized by skeletal, brain, and skin abnormalities. All people with this ...

The ancestry and affiliations of Kennewick Man.

PubMed

Rasmussen, Morten; Sikora, Martin; Albrechtsen, Anders; Korneliussen, Thorfinn Sand; Moreno-Mayar, J Víctor; Poznik, G David; Zollikofer, Christoph P E; de León, Marcia Ponce; Allentoft, Morten E; Moltke, Ida; Jónsson, Hákon; Valdiosera, Cristina; Malhi, Ripan S; Orlando, Ludovic; Bustamante, Carlos D; Stafford, Thomas W; Meltzer, David J; Nielsen, Rasmus; Willerslev, Eske

2015-07-23

Kennewick Man, referred to as the Ancient One by Native Americans, is a male human skeleton discovered in Washington state (USA) in 1996 and initially radiocarbon dated to 8,340-9,200 calibrated years before present (BP). His population affinities have been the subject of scientific debate and legal controversy. Based on an initial study of cranial morphology it was asserted that Kennewick Man was neither Native American nor closely related to the claimant Plateau tribes of the Pacific Northwest, who claimed ancestral relationship and requested repatriation under the Native American Graves Protection and Repatriation Act (NAGPRA). The morphological analysis was important to judicial decisions that Kennewick Man was not Native American and that therefore NAGPRA did not apply. Instead of repatriation, additional studies of the remains were permitted. Subsequent craniometric analysis affirmed Kennewick Man to be more closely related to circumpacific groups such as the Ainu and Polynesians than he is to modern Native Americans. In order to resolve Kennewick Man's ancestry and affiliations, we have sequenced his genome to ∼1× coverage and compared it to worldwide genomic data including for the Ainu and Polynesians. We find that Kennewick Man is closer to modern Native Americans than to any other population worldwide. Among the Native American groups for whom genome-wide data are available for comparison, several seem to be descended from a population closely related to that of Kennewick Man, including the Confederated Tribes of the Colville Reservation (Colville), one of the five tribes claiming Kennewick Man. We revisit the cranial analyses and find that, as opposed to genome-wide comparisons, it is not possible on that basis to affiliate Kennewick Man to specific contemporary groups. We therefore conclude based on genetic comparisons that Kennewick Man shows continuity with Native North Americans over at least the last eight millennia.

Some issues relating to the use of perfluorooctanesulfonate (PFOS) samples as reference standards.

PubMed

Arsenault, Gilles; Chittim, Brock; McAlees, Alan; McCrindle, Robert; Riddell, Nicole; Yeo, Brian

2008-01-01

Samples of potassium perfluorooctanesulfonate (PFOSK) from three suppliers were analyzed by LC-ESI-MS/MS for purity and by LC-ESI-MS for the percentage of linear isomer present. Our data indicated that the purity ranged from 80% to 98% and the percentages of linear isomer from 67% to 79%. The proportion of branched isomers present in the samples was also estimated using (19)F NMR. These results agreed quite closely with those found by LC-ESI-MS indicating that there is essentially no difference in overall SIM response factor for the branched isomers vs. that of the linear isomer. Several further observations relevant to the use of standards when analyzing for PFOS were encountered during this study. It appears unlikely that matrix effects attributable to the cation (sodium or potassium) present in PFOSNa or PFOSK internal standards is an issue. In seeking potential matrix effects, it was found that the chromatography was improved substantially when the standard was injected as a solution in 80:20 methanol/water rather than 100% methanol. Notably, in concert with the improvement in chromatography, an increase of about 10% in response was observed. In some closely related studies, when (18)O(2) mass-labeled perfluorohexanesulfonate was used as an internal standard, the actual and theoretical concentration ratios matched closely those for related native sulfonates as long as they did not co-elute. However, when they did co-elute, the peak intensities of the native species were enhanced by about 5%, while those of the labeled compound were suppressed by a similar amount. If this effect were not taken into account, the concentration of the native would be inflated by 10%.

Control method and system for hydraulic machines employing a dynamic joint motion model

DOEpatents

Danko, George [Reno, NV

2011-11-22

A control method and system for controlling a hydraulically actuated mechanical arm to perform a task, the mechanical arm optionally being a hydraulically actuated excavator arm. The method can include determining a dynamic model of the motion of the hydraulic arm for each hydraulic arm link by relating the input signal vector for each respective link to the output signal vector for the same link. Also the method can include determining an error signal for each link as the weighted sum of the differences between a measured position and a reference position and between the time derivatives of the measured position and the time derivatives of the reference position for each respective link. The weights used in the determination of the error signal can be determined from the constant coefficients of the dynamic model. The error signal can be applied in a closed negative feedback control loop to diminish or eliminate the error signal for each respective link.

Mental health problems in people living with HIV: changes in the last two decades: the London experience 1990–2014

PubMed Central

Adams, Catherine; Zacharia, Shilpa; Masters, Lisa; Coffey, Caroline; Catalan, Pepe

2016-01-01

ABSTRACT Mental health problems continue to be a significant comorbidity for people with HIV infection, even in the era of effective antiretroviral therapy. Here, we report on the changes in the mental health diagnoses based on clinical case reports amongst people with HIV referred to a specialist psychological medicine department over a 24-year period, which include the relative increase in depressive and anxiety disorders, often of a chronic nature, together with a decline in acute mental health syndromes, mania, and organic brain disorders. In addition, new challenges, like the presence of HIV and Hepatitis C co-infection, and the new problems created by recreational drugs, confirm the need for mental health services to be closely involved with the general medical services. A substantial proportion of people with HIV referred to specialist services suffer complex difficulties, which often require the collaboration of both psychiatrists and psychologists to deal effectively with their difficulties. PMID:26888472

Bibliographic search of the literature on lunar processing

NASA Technical Reports Server (NTRS)

Criswell, D. R.

1989-01-01

The Lunar and Planetary Institute (LPI) search produced approximately 80 references. Most of these are available at the LPI. The product includes only the author, title, date, and reference. The earliest (Cooper et al., Certain ecological aspects of a closed lunar base, Rand Corp.) is dated 6 March 1958. The latest is a semi-popular article (Cole and Majdacic, Astronomy, vol. 16) produced last year. The early references tend to be reports of government sponsored engineering studies. The later are generally semi-popular and science press articles. These references will be obtained on disk from the LPI and can be converted into an ASCII file for access via IBM-compatible PCs.

Use of the Primitive Unit Cell in Understanding Subtle Features of the Cubic Closest-Packed Structure

ERIC Educational Resources Information Center

Hawkins, John A.; Rittenhouse, Jeffrey L.; Soper, Linda M.; Rittenhouse, Robert C.

2008-01-01

One of the most important crystal structures adopted by metals is characterized by the "abcabc"...stacking of close-packed layers. This structure is commonly referred to in textbooks as the cubic close-packed (ccp) or face-centered cubic (fcc) structure, since the entire lattice can be generated by replication of a face-centered cubic unit cell…

Identifying and processing the gap between perceived and actual agreement in breast pathology interpretation.

PubMed

Carney, Patricia A; Allison, Kimberly H; Oster, Natalia V; Frederick, Paul D; Morgan, Thomas R; Geller, Berta M; Weaver, Donald L; Elmore, Joann G

2016-07-01

We examined how pathologists' process their perceptions of how their interpretations on diagnoses for breast pathology cases agree with a reference standard. To accomplish this, we created an individualized self-directed continuing medical education program that showed pathologists interpreting breast specimens how their interpretations on a test set compared with a reference diagnosis developed by a consensus panel of experienced breast pathologists. After interpreting a test set of 60 cases, 92 participating pathologists were asked to estimate how their interpretations compared with the standard for benign without atypia, atypia, ductal carcinoma in situ and invasive cancer. We then asked pathologists their thoughts about learning about differences in their perceptions compared with actual agreement. Overall, participants tended to overestimate their agreement with the reference standard, with a mean difference of 5.5% (75.9% actual agreement; 81.4% estimated agreement), especially for atypia and were least likely to overestimate it for invasive breast cancer. Non-academic affiliated pathologists were more likely to more closely estimate their performance relative to academic affiliated pathologists (77.6 vs 48%; P=0.001), whereas participants affiliated with an academic medical center were more likely to underestimate agreement with their diagnoses compared with non-academic affiliated pathologists (40 vs 6%). Before the continuing medical education program, nearly 55% (54.9%) of participants could not estimate whether they would overinterpret the cases or underinterpret them relative to the reference diagnosis. Nearly 80% (79.8%) reported learning new information from this individualized web-based continuing medical education program, and 23.9% of pathologists identified strategies they would change their practice to improve. In conclusion, when evaluating breast pathology specimens, pathologists do a good job of estimating their diagnostic agreement with a reference standard, but for atypia cases, pathologists tend to overestimate diagnostic agreement. Many participants were able to identify ways to improve.

Comparative analyses of quaternary arrangements in homo-oligomeric proteins in superfamilies: Functional implications.

PubMed

Sudha, Govindarajan; Srinivasan, Narayanaswamy

2016-09-01

A comprehensive analysis of the quaternary features of distantly related homo-oligomeric proteins is the focus of the current study. This study has been performed at the levels of quaternary state, symmetry, and quaternary structure. Quaternary state and quaternary structure refers to the number of subunits and spatial arrangements of subunits, respectively. Using a large dataset of available 3D structures of biologically relevant assemblies, we show that only 53% of the distantly related homo-oligomeric proteins have the same quaternary state. Considering these homologous homo-oligomers with the same quaternary state, conservation of quaternary structures is observed only in 38% of the pairs. In 36% of the pairs of distantly related homo-oligomers with different quaternary states the larger assembly in a pair shows high structural similarity with the entire quaternary structure of the related protein with lower quaternary state and it is referred as "Russian doll effect." The differences in quaternary state and structure have been suggested to contribute to the functional diversity. Detailed investigations show that even though the gross functions of many distantly related homo-oligomers are the same, finer level differences in molecular functions are manifested by differences in quaternary states and structures. Comparison of structures of biological assemblies in distantly and closely related homo-oligomeric proteins throughout the study differentiates the effects of sequence divergence on the quaternary structures and function. Knowledge inferred from this study can provide insights for improved protein structure classification and function prediction of homo-oligomers. Proteins 2016; 84:1190-1202. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Quality of life in relation to physical, psychosocial and socioeconomic conditions among reindeer-herding Sami.

PubMed

Daergo, Laila; Edin-Liljegren, Anette; Sjölander, Per

2008-02-01

To analyse different aspects of health-related quality of life factors among members of reindeer-herding families. Cross-sectional study based on data from a comprehensive survey. The health-related quality of life (SF-36) factors were analysed on 99 (56 men, 43 women) adult members of reindeer-herding families. Comparisons were made between the reindeer-herding family members and a Swedish reference population. Associations between mental and physical component summary measures and a number of sociodemographic, biomedical, physical, psychosocial and socio-economic variables were analysed with multivariate regression statistics. Men scored higher than women on physical and social function and vitality. The average scores on the subscales for the reindeer-herding family members were similar to those of the Swedish reference population, except for reindeer-herding men who scored higher on physical function and lower on bodily pain. For women, the quality of life was related to age, sense of coherence, lifestyle and behavioural variables, as well as to issues such as diseases among close relatives, social networks and the economy of their business. For men, it was mainly related to musculoskeletal pain conditions, age, sense of coherence and physical and psychosocial working conditions. Men and women of the reindeer-herding families need partly different conditions to enjoy a high quality of life. From the results, it might be predicted that poor somatic and psychosocial health, increased intrusion from exploiters on the grazing land and declining profit in reindeer husbandry constitute important threats to a good quality of life among members of reindeer-herding families.

Image matching for digital close-range stereo photogrammetry based on constraints of Delaunay triangulated network and epipolar-line

NASA Astrophysics Data System (ADS)

Zhang, K.; Sheng, Y. H.; Li, Y. Q.; Han, B.; Liang, Ch.; Sha, W.

2006-10-01

In the field of digital photogrammetry and computer vision, the determination of conjugate points in a stereo image pair, referred to as "image matching," is the critical step to realize automatic surveying and recognition. Traditional matching methods encounter some problems in the digital close-range stereo photogrammetry, because the change of gray-scale or texture is not obvious in the close-range stereo images. The main shortcoming of traditional matching methods is that geometric information of matching points is not fully used, which will lead to wrong matching results in regions with poor texture. To fully use the geometry and gray-scale information, a new stereo image matching algorithm is proposed in this paper considering the characteristics of digital close-range photogrammetry. Compared with the traditional matching method, the new algorithm has three improvements on image matching. Firstly, shape factor, fuzzy maths and gray-scale projection are introduced into the design of synthetical matching measure. Secondly, the topology connecting relations of matching points in Delaunay triangulated network and epipolar-line are used to decide matching order and narrow the searching scope of conjugate point of the matching point. Lastly, the theory of parameter adjustment with constraint is introduced into least square image matching to carry out subpixel level matching under epipolar-line constraint. The new algorithm is applied to actual stereo images of a building taken by digital close-range photogrammetric system. The experimental result shows that the algorithm has a higher matching speed and matching accuracy than pyramid image matching algorithm based on gray-scale correlation.

Fuel Composition Effects at Constant RON and MON in an HCCI Engine Operated with Negative Valve Overlap

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bunting, Bruce G; Farrell, John T

2006-01-01

The effects of fuel properties on gasoline HCCI operation have been investigated in a single cylinder, 500 cc, 11.3 CR port fuel injected research engine, operated at lambda=1 and equipped with hydraulic valve actuation. HCCI is promoted by early exhaust valve closing to retain hot exhaust in the cylinder, thereby increasing the cylinder gas temperature. Test fuels were formulated with pure components to have the same RON, MON, and octane sensitivity as an indolene reference fuel, but with a wide range of fuel composition differences. Experiments have been carried out to determine if fuel composition plays a role in HCCImore » combustion properties, independent of octane numbers. Fuel economy, emissions, and combustion parameters have been measured at several fixed speed/load conditions over a range of exhaust valve closing angles. When the data are compared at constant combustion phasing, fuel effects on emissions and other combustion properties are small. However, when compared at constant exhaust valve closing angle, fuel composition effects are more pronounced, specifically regarding ignition. Operability range differences are also related to fuel composition. An all-paraffinic (normal, iso, and cycloparaffins) fuel exhibited distinctly earlier combustion phasing, increased rate of cylinder pressure rise, and increased rate of maximum heat release compared to the indolene reference fuel. Conversely, olefin-containing fuels exhibited retarded combustion phasing. The fuels with the most advanced ignition showed a wider operating range in terms of engine speed and load, irrespective of exhaust closing angle. These ignition differences reflect contributions from both fuel and EGR kinetics, the effects of which are discussed. The fuel composition variables are somewhat inter-correlated, which makes the experimental separation their effects imprecise with this small set of fuels, though clear trends are evident. The overall effects of fuel composition on engine performance and emissions are small. However, the results suggest that the effects on combustion phasing and engine operability range may need to be considered in the practical implementation of HCCI for fuels with large compositional variations.« less

Device for measuring oxygen activity in liquid sodium

DOEpatents

Roy, P.; Young, R.S.

1973-12-01

A composite ceramic electrolyte in a configuration (such as a closed end tube or a plate) suitable to separate liquid sodium from a reference electrode with a high impedance voltmeter connected to measure EMF between the sodium and the reference electrode as a measure of oxygen activity in the sodium is described. The composite electrolyte consists of zirconiacalcia with a bonded layer of thoria-yttria. The device is used with a gaseous reference electrode on the zirconia-calcia side and liquid sodium on the thoria-yttria side of the electrolyte. (Official Gazette)

Design of ultra-low power biopotential amplifiers for biosignal acquisition applications.

PubMed

Zhang, Fan; Holleman, Jeremy; Otis, Brian P

2012-08-01

Rapid development in miniature implantable electronics are expediting advances in neuroscience by allowing observation and control of neural activities. The first stage of an implantable biosignal recording system, a low-noise biopotential amplifier (BPA), is critical to the overall power and noise performance of the system. In order to integrate a large number of front-end amplifiers in multichannel implantable systems, the power consumption of each amplifier must be minimized. This paper introduces a closed-loop complementary-input amplifier, which has a bandwidth of 0.05 Hz to 10.5 kHz, an input-referred noise of 2.2 μ Vrms, and a power dissipation of 12 μW. As a point of comparison, a standard telescopic-cascode closed-loop amplifier with a 0.4 Hz to 8.5 kHz bandwidth, input-referred noise of 3.2 μ Vrms, and power dissipation of 12.5 μW is presented. Also for comparison, we show results from an open-loop complementary-input amplifier that exhibits an input-referred noise of 3.6 μ Vrms while consuming 800 nW of power. The two closed-loop amplifiers are fabricated in a 0.13 μ m CMOS process. The open-loop amplifier is fabricated in a 0.5 μm SOI-BiCMOS process. All three amplifiers operate with a 1 V supply.

Terrain Correction on the moving equal area cylindrical map projection of the surface of a reference ellipsoid

NASA Astrophysics Data System (ADS)

Ardalan, A.; Safari, A.; Grafarend, E.

2003-04-01

An operational algorithm for computing the ellipsoidal terrain correction based on application of closed form solution of the Newton integral in terms of Cartesian coordinates in the cylindrical equal area map projected surface of a reference ellipsoid has been developed. As the first step the mapping of the points on the surface of a reference ellipsoid onto the cylindrical equal area map projection of a cylinder tangent to a point on the surface of reference ellipsoid closely studied and the map projection formulas are computed. Ellipsoidal mass elements with various sizes on the surface of the reference ellipsoid is considered and the gravitational potential and the vector of gravitational intensity of these mass elements has been computed via the solution of Newton integral in terms of ellipsoidal coordinates. The geographical cross section areas of the selected ellipsoidal mass elements are transferred into cylindrical equal area map projection and based on the transformed area elements Cartesian mass elements with the same height as that of the ellipsoidal mass elements are constructed. Using the close form solution of the Newton integral in terms of Cartesian coordinates the potential of the Cartesian mass elements are computed and compared with the same results based on the application of the ellipsoidal Newton integral over the ellipsoidal mass elements. The results of the numerical computations show that difference between computed gravitational potential of the ellipsoidal mass elements and Cartesian mass element in the cylindrical equal area map projection is of the order of 1.6 × 10-8m^2/s^2 for a mass element with the cross section size of 10 km × 10 km and the height of 1000 m. For a 1 km × 1 km mass element with the same height, this difference is less than 1.5 × 10-4 m^2}/s^2. The results of the numerical computations indicate that a new method for computing the terrain correction based on the closed form solution of the Newton integral in terms of Cartesian coordinates and with accuracy of ellipsoidal terrain correction has been achieved! In this way one can enjoy the simplicity of the solution of the Newton integral in terms of Cartesian coordinates and at the same time the accuracy of the ellipsoidal terrain correction, which is needed for the modern theory of geoid computations.

Stats Don't Tell the Whole Story: Using Qualitative Data Analysis of Chat Reference Transcripts to Assess and Improve Services

ERIC Educational Resources Information Center

Mungin, Michael

2017-01-01

In the five years following implementation of a chat reference service at James Madison University (JMU), the service proved very popular but was not closely assessed for quality of service. Using grounded theory and qualitative data analysis techniques, a comprehensive assessment effort was begun in earnest and is in progress. Preliminary results…

Variation in the Use of Therapy following Distal Radius Fractures in the United States

PubMed Central

Waljee, Jennifer F.; Zhong, Lin; Shauver, Melissa

2014-01-01

Background: Distal radius fractures (DRFs) are one of the most common injuries among the elderly, resulting in significant expense and disability. The specific aims of this study are (1) to examine rates of therapy following DRFs and (2) to identify those factors that influence utilization of therapy and time span between DRF treatment and therapy among a national cohort of elderly patients. Methods: We examined national use of physical and occupational therapy among all Medicare beneficiaries who suffered DRFs between January 1, 2007, and October 1, 2007, and assessed the effect of treatment, patient-related, and surgeon-related factors on utilization of therapy. Results: Overall, 20.6% of patients received either physical or occupational therapy following DRF. Use of therapy varied by DRF treatment, and patients who underwent open reduction and internal fixation were more likely to receive therapy compared with patients who received closed reduction. Patients who received open reduction and internal fixation were also referred to therapy earlier compared with patients who received external fixation, percutaneous pinning, and closed reduction. Surgeon specialization is associated with greater use of postoperative therapy. Patient predictors of therapy use include younger age, female sex, higher socioeconomic status, and fewer comorbidity conditions. Conclusion: Use of therapy following DRF varies significantly by both patient- and surgeon-related factors. Identifying patients who benefit from postinjury therapy can allow for better resource utilization following these common injuries. PMID:25289323

Plantactinospora sonchi sp. nov., an actinobacterium isolated from the leaves of common sowthistle (Sonchus oleraceus L.).

PubMed

Ma, Zhaoxu; Liu, Chongxi; Fan, Jianlong; He, Hairong; Li, Chuang; Li, Jiansong; Zhao, Shanshan; Xiang, Wensheng; Wang, Xiangjing

2015-12-01

A novel actinobacterium, designated strain NEAU-QY2T, was isolated from the leaves of Sonchus oleraceus L. specimen, collected from Wuchang, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic position of this strain. The organism formed single spores with rough surfaces on substrate mycelia. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-QY2T belonged to the genus Plantactinospora and formed a monophyletic clade with its closest related strains Plantactinospora endophytica YIM 68255T (99.2 % 16S rRNA gene sequence similarity), Plantactinospora veratri NEAU-FHS4T (98.8 %) and Plantactinospora mayteni YIM 61359T(98.7 %), an association that was supported by a bootstrap value of 90 % in the neighbor-joining tree and also recovered with the maximum-likelihood algorithm. However, DNA-DNA hybridization values between strain NEAU-QY2T and the three closely related strains were below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, strain NEAU-QY2T was distinguished from closely related strains and is classified as representing a novel species of the genus Plantactinospora, for which the name Plantactinospora sonchi sp. nov. is proposed. The type strain is NEAU-QY2T (=CGMCC4.7216T=JCM 30345T).

Remote Sensing Analysis of Temperature and Suspended Sediment Concentration in Ayeyarwady River in Myanmar

NASA Astrophysics Data System (ADS)

Thanda Ko, Nyein; Rutten, Martine

2017-04-01

Detailed spatial coverage of water quality parameters are crucial to better manage rivers. However, collection of water quality parameters is both time consuming and costly for large rivers. This study demonstrates that Operational Land Image (OLI) Sensor on board of Landsat 8 can be successfully applied for the detection of spatial patterns of water temperature as well as suspended sediment concentration (SSC) using the Ayeyarwady river, Myanmar as a case study. Water temperature estimation was obtained from the brightness thermal Band 10 by using the Split-Window algorithm. The study finds that there is a close agreement between the remote sensing temperature and in-situ temperature with relative error in the range from 4.5% to 8.2%. The sediment load of Ayeyarwady river is ranked as the third-largest sediment load among the world's rivers but there is very little known about this important parameter, due to a lack of adequate gauge data. The single band reflectance of Landsat image (Band 5) seems a good indicator for the estimation of SSC with relative error in the range of less than 10% but the developed empirical formula by the power relation with the only seven ground reference points is uncertain to apply for the entire river basin. It is to note that an important constraint for the sediment analysis is the availability of spatial and temporal ground reference data. Future studies should also focus on the improvement of ground reference data points to become more reliable, because most of the river in Asia, especially in Myanmar, don't have readily available continuous ground sediment data points due to lack of measurement gauge stations through the river.

Nutrient limitation drives response of Calamagrostis epigejos to arbuscular mycorrhiza in primary succession.

PubMed

Rydlová, Jana; Püschel, David; Dostálová, Magdalena; Janoušková, Martina; Frouz, Jan

2016-10-01

Little is known about the functioning of arbuscular mycorrhizal (AM) symbiosis over the course of primary succession, where soil, host plants, and AM fungal communities all undergo significant changes. Over the course of succession at the studied post-mining site, plant cover changes from an herbaceous community to the closed canopy of a deciduous forest. Calamagrostis epigejos (Poaceae) is a common denominator at all stages, and it dominates among AM host species. Its growth response to AM fungi was studied at four distinctive stages of natural succession: 12, 20, 30, and 50 years of age, each represented by three spatially separated sites. Soils obtained from all 12 studied sites were γ-sterilized and used in a greenhouse experiment in which C. epigejos plants were (1) inoculated with a respective community of native AM fungi, (2) inoculated with reference AM fungal isolates from laboratory collection, or (3) cultivated without AM fungi. AM fungi strongly boosted plant growth during the first two stages but not during the latter two, where the effect was neutral or even negative. While plant phosphorus (P) uptake was generally increased by AM fungi, no contribution of mycorrhizae to nitrogen (N) uptake was recorded. Based on N:P in plant biomass, we related the turn from a positive to a neutral/negative effect of AM fungi on plant growth, observed along the chronosequence, to a shift in relative P and N availability. No functional differences were found between native and reference inocula, yet root colonization by the native AM fungi decreased relative to the reference inoculum in the later succession stages, thereby indicating shifts in the composition of AM fungal communities reflected in different functional characteristics of their members.

African great apes are natural hosts of multiple related malaria species, including Plasmodium falciparum

PubMed Central

Prugnolle, Franck; Durand, Patrick; Neel, Cécile; Ollomo, Benjamin; Ayala, Francisco J.; Arnathau, Céline; Etienne, Lucie; Mpoudi-Ngole, Eitel; Nkoghe, Dieudonné; Leroy, Eric; Delaporte, Eric; Peeters, Martine; Renaud, François

2010-01-01

Plasmodium reichenowi, a chimpanzee parasite, was until very recently the only known close relative of Plasmodium falciparum, the most virulent agent of human malaria. Recently, Plasmodium gaboni, another closely related chimpanzee parasite, was discovered, suggesting that the diversity of Plasmodium circulating in great apes in Africa might have been underestimated. It was also recently shown that P. reichenowi is a geographically widespread and genetically diverse chimpanzee parasite and that the world diversity of P. falciparum is fully included within the much broader genetic diversity of P. reichenowi. The evidence indicates that all extant populations of P. falciparum originated from P. reichenowi, likely by a single transfer from chimpanzees. In this work, we have studied the diversity of Plasmodium species infecting chimpanzees and gorillas in Central Africa (Cameroon and Gabon) from both wild-living and captive animals. The studies in wild apes used noninvasive sampling methods. We confirm the presence of P. reichenowi and P. gaboni in wild chimpanzees. Moreover, our results reveal the existence of an unexpected genetic diversity of Plasmodium lineages circulating in gorillas. We show that gorillas are naturally infected by two related lineages of parasites that have not been described previously, herein referred to as Plasmodium GorA and P. GorB, but also by P. falciparum, a species previously considered as strictly human specific. The continuously increasing contacts between humans and primate populations raise concerns about further reciprocal host transfers of these pathogens. PMID:20133889

Equine-related treatments for mental disorders lack empirical support: a systematic review of empirical investigations.

PubMed

Anestis, Michael D; Anestis, Joye C; Zawilinski, Laci L; Hopkins, Tiffany A; Lilienfeld, Scott O

2014-12-01

Equine-related treatments (ERT) for mental disorders are becoming increasingly popular for a variety of diagnoses; however, they have been subjected only to limited systematic investigation. To examine the quality of and results from peer-reviewed research on ERT for mental disorders and related outcomes. Peer-reviewed studies (k = 14) examining treatments for mental disorders or closely related outcomes were identified from databases and article reference sections. All studies were compromised by a substantial number of threats to validity, calling into question the meaning and clinical significance of their findings. Additionally, studies failed to provide consistent evidence that ERT is superior to the mere passage of time in the treatment of any mental disorder. The current evidence base does not justify the marketing and utilization of ERT for mental disorders. Such services should not be offered to the public unless and until well-designed studies provide evidence that justify different conclusions. © 2014 Wiley Periodicals, Inc.

Metals in agricultural produce associated with acid-mine drainage in Mount Morgan (Queensland, Australia).

PubMed

Vicente-Beckett, Victoria A; McCauley, Gaylene J Taylor; Duivenvoorden, Leo J

2016-01-01

Acid-mine drainage (AMD) into the Dee River from the historic gold and copper mine in Mount Morgan, Queensland (Australia) has been of concern to farmers in the area since 1925. This study sought to determine the levels of AMD-related metals and sulfur in agricultural produce grown near the mine-impacted Dee River, compare these with similar produce grown in reference fields (which had no known AMD influence), and assess any potential health risk using relevant Australian or US guidelines. Analyses of lucerne (Medicago sativa; also known as alfalfa) from five Dee fields showed the following average concentrations (mg/kg dry basis): Cd < 1, Cu 11, Fe 106, Mn 52, Pb < 5, Zn 25 and S 3934; similar levels were found in lucerne hay (used as cattle feed) from two Dee fields. All lucerne and lucerne hay data were generally comparable with levels found in the lucerne reference fields, suggesting no AMD influence; the levels were within the US National Research Council (US NRC) guidelines for maximum tolerable cattle dietary intake. Pasture grass (also cattle feed) from two fields in the Dee River floodplains gave mean concentrations (mg/kg dry) of Cd 0.14, Cu 12, Fe 313, Mn 111, Pb 1.4, Zn 86 and S 2450. All metal levels from the Dee and from reference sites were below the US NRC guidelines for maximum tolerable cattle dietary intake; however, the average Cd, Cu and Fe levels in Dee samples were significantly greater than the corresponding levels in the pasture grass reference sites, suggesting AMD influence in the Dee samples. The average levels in the edible portions of mandarin oranges (Citrus reticulata) from Dee sites (mg/kg wet weight) were Cd 0.011, Cu 0.59, Fe 2.2, Mn 0.56, Pb 0.18, S 91 and Zn 0.96. Cd and Zn were less than or close to, average Fe and Mn levels were at most twice, Cd 1.8 or 6.5 times, and Pb 8.5 or 72 times the maximum levels in raw oranges reported in the US total diet study (TDS) or the Australian TDS, respectively. Average Cd, Fe, Mn, Pb and Zn levels in the citrus reference samples were found to exceed the maximum reported in one or both TDS surveys. Cu, Fe, Mn, Pb and Zn plant-soil transfer factor (TF) values were < 1 for all agricultural samples from both Dee and reference sites, suggesting relatively poor transfer of these metals from soil to plant. In the case of Cd, TF values for Dee pasture grass and citrus fruit samples were 0.14 and 0.73, respectively; lucerne and lucerne hay from both Dee and reference sites gave TF = 10, suggesting some potential risk to cattle, although this conclusion is tentative because Cd levels were close to or less than the detection limit. TF values for S in lucerne, lucerne hay, pasture grass and mandarin oranges from Dee sites were 18, 14, 3 and 3.6, respectively, indicating that S in soil was readily available to plant or fruit. Sulfur in pasture grass and citrus fruit (TF = 11 for both) was apparently more bioavailable at the reference sites than at the Dee sites (TF = 3.0 for pasture grass; TF = 3.6 for citrus fruit).

Ethical issues in using Twitter for public health surveillance and research: developing a taxonomy of ethical concepts from the research literature.

PubMed

Conway, Mike

2014-12-22

The rise of social media and microblogging platforms in recent years, in conjunction with the development of techniques for the processing and analysis of "big data", has provided significant opportunities for public health surveillance using user-generated content. However, relatively little attention has been focused on developing ethically appropriate approaches to working with these new data sources. Based on a review of the literature, this study seeks to develop a taxonomy of public health surveillance-related ethical concepts that emerge when using Twitter data, with a view to: (1) explicitly identifying a set of potential ethical issues and concerns that may arise when researchers work with Twitter data, and (2) providing a starting point for the formation of a set of best practices for public health surveillance through the development of an empirically derived taxonomy of ethical concepts. We searched Medline, Compendex, PsycINFO, and the Philosopher's Index using a set of keywords selected to identify Twitter-related research papers that reference ethical concepts. Our initial set of queries identified 342 references across the four bibliographic databases. We screened titles and abstracts of these references using our inclusion/exclusion criteria, eliminating duplicates and unavailable papers, until 49 references remained. We then read the full text of these 49 articles and discarded 36, resulting in a final inclusion set of 13 articles. Ethical concepts were then identified in each of these 13 articles. Finally, based on a close reading of the text, a taxonomy of ethical concepts was constructed based on ethical concepts discovered in the papers. From these 13 articles, we iteratively generated a taxonomy of ethical concepts consisting of 10 top level categories: privacy, informed consent, ethical theory, institutional review board (IRB)/regulation, traditional research vs Twitter research, geographical information, researcher lurking, economic value of personal information, medical exceptionalism, and benefit of identifying socially harmful medical conditions. In summary, based on a review of the literature, we present a provisional taxonomy of public health surveillance-related ethical concepts that emerge when using Twitter data.

Ethical Issues in Using Twitter for Public Health Surveillance and Research: Developing a Taxonomy of Ethical Concepts From the Research Literature

PubMed Central

2014-01-01

Background The rise of social media and microblogging platforms in recent years, in conjunction with the development of techniques for the processing and analysis of “big data”, has provided significant opportunities for public health surveillance using user-generated content. However, relatively little attention has been focused on developing ethically appropriate approaches to working with these new data sources. Objective Based on a review of the literature, this study seeks to develop a taxonomy of public health surveillance-related ethical concepts that emerge when using Twitter data, with a view to: (1) explicitly identifying a set of potential ethical issues and concerns that may arise when researchers work with Twitter data, and (2) providing a starting point for the formation of a set of best practices for public health surveillance through the development of an empirically derived taxonomy of ethical concepts. Methods We searched Medline, Compendex, PsycINFO, and the Philosopher’s Index using a set of keywords selected to identify Twitter-related research papers that reference ethical concepts. Our initial set of queries identified 342 references across the four bibliographic databases. We screened titles and abstracts of these references using our inclusion/exclusion criteria, eliminating duplicates and unavailable papers, until 49 references remained. We then read the full text of these 49 articles and discarded 36, resulting in a final inclusion set of 13 articles. Ethical concepts were then identified in each of these 13 articles. Finally, based on a close reading of the text, a taxonomy of ethical concepts was constructed based on ethical concepts discovered in the papers. Results From these 13 articles, we iteratively generated a taxonomy of ethical concepts consisting of 10 top level categories: privacy, informed consent, ethical theory, institutional review board (IRB)/regulation, traditional research vs Twitter research, geographical information, researcher lurking, economic value of personal information, medical exceptionalism, and benefit of identifying socially harmful medical conditions. Conclusions In summary, based on a review of the literature, we present a provisional taxonomy of public health surveillance-related ethical concepts that emerge when using Twitter data. PMID:25533619

Mechanism reduction for multicomponent surrogates: A case study using toluene reference fuels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Niemeyer, Kyle E.; Sung, Chih-Jen

Strategies and recommendations for performing skeletal reductions of multicomponent surrogate fuels are presented, through the generation and validation of skeletal mechanisms for a three-component toluene reference fuel. Using the directed relation graph with error propagation and sensitivity analysis method followed by a further unimportant reaction elimination stage, skeletal mechanisms valid over comprehensive and high-temperature ranges of conditions were developed at varying levels of detail. These skeletal mechanisms were generated based on autoignition simulations, and validation using ignition delay predictions showed good agreement with the detailed mechanism in the target range of conditions. When validated using phenomena other than autoignition, suchmore » as perfectly stirred reactor and laminar flame propagation, tight error control or more restrictions on the reduction during the sensitivity analysis stage were needed to ensure good agreement. In addition, tight error limits were needed for close prediction of ignition delay when varying the mixture composition away from that used for the reduction. In homogeneous compression-ignition engine simulations, the skeletal mechanisms closely matched the point of ignition and accurately predicted species profiles for lean to stoichiometric conditions. Furthermore, the efficacy of generating a multicomponent skeletal mechanism was compared to combining skeletal mechanisms produced separately for neat fuel components; using the same error limits, the latter resulted in a larger skeletal mechanism size that also lacked important cross reactions between fuel components. Based on the present results, general guidelines for reducing detailed mechanisms for multicomponent fuels are discussed.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eriksen, Janus J., E-mail: janusje@chem.au.dk; Jørgensen, Poul; Matthews, Devin A.

The accuracy at which total energies of open-shell atoms and organic radicals may be calculated is assessed for selected coupled cluster perturbative triples expansions, all of which augment the coupled cluster singles and doubles (CCSD) energy by a non-iterative correction for the effect of triple excitations. Namely, the second- through sixth-order models of the recently proposed CCSD(T–n) triples series [J. J. Eriksen et al., J. Chem. Phys. 140, 064108 (2014)] are compared to the acclaimed CCSD(T) model for both unrestricted as well as restricted open-shell Hartree-Fock (UHF/ROHF) reference determinants. By comparing UHF- and ROHF-based statistical results for a test setmore » of 18 modest-sized open-shell species with comparable RHF-based results, no behavioral differences are observed for the higher-order models of the CCSD(T–n) series in their correlated descriptions of closed- and open-shell species. In particular, we find that the convergence rate throughout the series towards the coupled cluster singles, doubles, and triples (CCSDT) solution is identical for the two cases. For the CCSD(T) model, on the other hand, not only its numerical consistency, but also its established, yet fortuitous cancellation of errors breaks down in the transition from closed- to open-shell systems. The higher-order CCSD(T–n) models (orders n > 3) thus offer a consistent and significant improvement in accuracy relative to CCSDT over the CCSD(T) model, equally for RHF, UHF, and ROHF reference determinants, albeit at an increased computational cost.« less

Mechanism reduction for multicomponent surrogates: A case study using toluene reference fuels

DOE PAGES

Niemeyer, Kyle E.; Sung, Chih-Jen

2014-11-01

Strategies and recommendations for performing skeletal reductions of multicomponent surrogate fuels are presented, through the generation and validation of skeletal mechanisms for a three-component toluene reference fuel. Using the directed relation graph with error propagation and sensitivity analysis method followed by a further unimportant reaction elimination stage, skeletal mechanisms valid over comprehensive and high-temperature ranges of conditions were developed at varying levels of detail. These skeletal mechanisms were generated based on autoignition simulations, and validation using ignition delay predictions showed good agreement with the detailed mechanism in the target range of conditions. When validated using phenomena other than autoignition, suchmore » as perfectly stirred reactor and laminar flame propagation, tight error control or more restrictions on the reduction during the sensitivity analysis stage were needed to ensure good agreement. In addition, tight error limits were needed for close prediction of ignition delay when varying the mixture composition away from that used for the reduction. In homogeneous compression-ignition engine simulations, the skeletal mechanisms closely matched the point of ignition and accurately predicted species profiles for lean to stoichiometric conditions. Furthermore, the efficacy of generating a multicomponent skeletal mechanism was compared to combining skeletal mechanisms produced separately for neat fuel components; using the same error limits, the latter resulted in a larger skeletal mechanism size that also lacked important cross reactions between fuel components. Based on the present results, general guidelines for reducing detailed mechanisms for multicomponent fuels are discussed.« less

Subsampled open-reference clustering creates consistent, comprehensive OTU definitions and scales to billions of sequences.

PubMed

Rideout, Jai Ram; He, Yan; Navas-Molina, Jose A; Walters, William A; Ursell, Luke K; Gibbons, Sean M; Chase, John; McDonald, Daniel; Gonzalez, Antonio; Robbins-Pianka, Adam; Clemente, Jose C; Gilbert, Jack A; Huse, Susan M; Zhou, Hong-Wei; Knight, Rob; Caporaso, J Gregory

2014-01-01

We present a performance-optimized algorithm, subsampled open-reference OTU picking, for assigning marker gene (e.g., 16S rRNA) sequences generated on next-generation sequencing platforms to operational taxonomic units (OTUs) for microbial community analysis. This algorithm provides benefits over de novo OTU picking (clustering can be performed largely in parallel, reducing runtime) and closed-reference OTU picking (all reads are clustered, not only those that match a reference database sequence with high similarity). Because more of our algorithm can be run in parallel relative to "classic" open-reference OTU picking, it makes open-reference OTU picking tractable on massive amplicon sequence data sets (though on smaller data sets, "classic" open-reference OTU clustering is often faster). We illustrate that here by applying it to the first 15,000 samples sequenced for the Earth Microbiome Project (1.3 billion V4 16S rRNA amplicons). To the best of our knowledge, this is the largest OTU picking run ever performed, and we estimate that our new algorithm runs in less than 1/5 the time than would be required of "classic" open reference OTU picking. We show that subsampled open-reference OTU picking yields results that are highly correlated with those generated by "classic" open-reference OTU picking through comparisons on three well-studied datasets. An implementation of this algorithm is provided in the popular QIIME software package, which uses uclust for read clustering. All analyses were performed using QIIME's uclust wrappers, though we provide details (aided by the open-source code in our GitHub repository) that will allow implementation of subsampled open-reference OTU picking independently of QIIME (e.g., in a compiled programming language, where runtimes should be further reduced). Our analyses should generalize to other implementations of these OTU picking algorithms. Finally, we present a comparison of parameter settings in QIIME's OTU picking workflows and make recommendations on settings for these free parameters to optimize runtime without reducing the quality of the results. These optimized parameters can vastly decrease the runtime of uclust-based OTU picking in QIIME.

A Model for Developing Clinical Analytics Capacity: Closing the Loops on Outcomes to Optimize Quality.

PubMed

Eggert, Corinne; Moselle, Kenneth; Protti, Denis; Sanders, Dale

2017-01-01

Closed Loop Analytics© is receiving growing interest in healthcare as a term referring to information technology, local data and clinical analytics working together to generate evidence for improvement. The Closed Loop Analytics model consists of three loops corresponding to the decision-making levels of an organization and the associated data within each loop - Patients, Protocols, and Populations. The authors propose that each of these levels should utilize the same ecosystem of electronic health record (EHR) and enterprise data warehouse (EDW) enabled data, in a closed-loop fashion, with that data being repackaged and delivered to suit the analytic and decision support needs of each level, in support of better outcomes.

Multimodal Fusion with Reference: Searching for Joint Neuromarkers of Working Memory Deficits in Schizophrenia

PubMed Central

Qi, Shile; Calhoun, Vince D.; van Erp, Theo G. M.; Bustillo, Juan; Damaraju, Eswar; Turner, Jessica A.; Du, Yuhui; Chen, Jiayu; Yu, Qingbao; Mathalon, Daniel H.; Ford, Judith M.; Voyvodic, James; Mueller, Bryon A.; Belger, Aysenil; Ewen, Sarah Mc; Potkin, Steven G.; Preda, Adrian; Jiang, Tianzi

2017-01-01

Multimodal fusion is an effective approach to take advantage of cross-information among multiple imaging data to better understand brain diseases. However, most current fusion approaches are blind, without adopting any prior information. To date, there is increasing interest to uncover the neurocognitive mapping of specific behavioral measurement on enriched brain imaging data; hence, a supervised, goal-directed model that enables a priori information as a reference to guide multimodal data fusion is in need and a natural option. Here we proposed a fusion with reference model, called “multi-site canonical correlation analysis with reference plus joint independent component analysis” (MCCAR+jICA), which can precisely identify co-varying multimodal imaging patterns closely related to reference information, such as cognitive scores. In a 3-way fusion simulation, the proposed method was compared with its alternatives on estimation accuracy of both target component decomposition and modality linkage detection. MCCAR+jICA outperforms others with higher precision. In human imaging data, working memory performance was utilized as a reference to investigate the covarying functional and structural brain patterns among 3 modalities and how they are impaired in schizophrenia. Two independent cohorts (294 and 83 subjects respectively) were used. Interestingly, similar brain maps were identified between the two cohorts, with substantial overlap in the executive control networks in fMRI, salience network in sMRI, and major white matter tracts in dMRI. These regions have been linked with working memory deficits in schizophrenia in multiple reports, while MCCAR+jICA further verified them in a repeatable, joint manner, demonstrating the potential of such results to identify potential neuromarkers for mental disorders. PMID:28708547

Validation of Reference Genes for Quantitative Expression Analysis by Real-Time RT-PCR in Four Lepidopteran Insects

PubMed Central

Teng, Xiaolu; Zhang, Zan; He, Guiling; Yang, Liwen; Li, Fei

2012-01-01

Quantitative real-time polymerase chain reaction (qPCR) is an efficient and widely used technique to monitor gene expression. Housekeeping genes (HKGs) are often empirically selected as the reference genes for data normalization. However, the suitability of HKGs used as the reference genes has been seldom validated. Here, six HKGs were chosen (actin A3, actin A1, GAPDH, G3PDH, E2F, rp49) in four lepidopteran insects Bombyx mori L. (Lepidoptera: Bombycidae), Plutella xylostella L. (Plutellidae), Chilo suppressalis Walker (Crambidae), and Spodoptera exigua Hübner (Noctuidae) to study their expression stability. The algorithms of geNorm, NormFinder, stability index, and ΔCt analysis were used to evaluate these HKGs. Across different developmental stages, actin A1 was the most stable in P. xylostella and C. suppressalis, but it was the least stable in B. mori and S. exigua. Rp49 and GAPDH were the most stable in B. mori and S. exigua, respectively. In different tissues, GAPDH, E2F, and Rp49 were the most stable in B. mori, S. exigua, and C. suppressalis, respectively. The relative abundances of Siwi genes estimated by 2-ΔΔCt method were tested with different HKGs as the reference gene, proving the importance of internal controls in qPCR data analysis. The results not only presented a list of suitable reference genes in four lepidopteran insects, but also proved that the expression stabilities of HKGs were different among evolutionarily close species. There was no single universal reference gene that could be used in all situations. It is indispensable to validate the expression of HKGs before using them as the internal control in qPCR. PMID:22938136

Validation of reference genes for quantitative expression analysis by real-time rt-PCR in four lepidopteran insects.

PubMed

Teng, Xiaolu; Zhang, Zan; He, Guiling; Yang, Liwen; Li, Fei

2012-01-01

Quantitative real-time polymerase chain reaction (qPCR) is an efficient and widely used technique to monitor gene expression. Housekeeping genes (HKGs) are often empirically selected as the reference genes for data normalization. However, the suitability of HKGs used as the reference genes has been seldom validated. Here, six HKGs were chosen (actin A3, actin A1, GAPDH, G3PDH, E2F, rp49) in four lepidopteran insects Bombyx mori L. (Lepidoptera: Bombycidae), Plutella xylostella L. (Plutellidae), Chilo suppressalis Walker (Crambidae), and Spodoptera exigua Hübner (Noctuidae) to study their expression stability. The algorithms of geNorm, NormFinder, stability index, and ΔCt analysis were used to evaluate these HKGs. Across different developmental stages, actin A1 was the most stable in P. xylostella and C. suppressalis, but it was the least stable in B. mori and S. exigua. Rp49 and GAPDH were the most stable in B. mori and S. exigua, respectively. In different tissues, GAPDH, E2F, and Rp49 were the most stable in B. mori, S. exigua, and C. suppressalis, respectively. The relative abundances of Siwi genes estimated by 2(-ΔΔCt) method were tested with different HKGs as the reference gene, proving the importance of internal controls in qPCR data analysis. The results not only presented a list of suitable reference genes in four lepidopteran insects, but also proved that the expression stabilities of HKGs were different among evolutionarily close species. There was no single universal reference gene that could be used in all situations. It is indispensable to validate the expression of HKGs before using them as the internal control in qPCR.

Geomorphological reference condition definition as a basis for river restoration and river management: the example of Oiartzun, Oria and Urumea River basins (Basque Country)

NASA Astrophysics Data System (ADS)

Ibisate, Askoa; Ollero, Alfredo; Sáenz de Olazagoitia, Ana; Acín, Vanesa; Granado, David; Herrero, Xabier; Horacio, Jesús

2017-04-01

The application of hydrogeomorphology as a tool for river management and decision making on reference condition definition for river restoration is presented. Water Framework Directive (2000/60/CE) requires the identification of reference conditions and attainable target images, to achieve the good ecological status, taking into account the direct and indirect changes in the basin and river course. Data collection was done through an exhaustive fieldwork and GIS tools. Based on geomorphological homogeneous river reaches identification (waterfall, bedrock, step-pool, cascade, coluvial, run, riffle-pool, heavily modified), the hydrogeomorphological assessment of all of them in relation to its "natural" condition allowed the identification of those with a good or very good hydrogeomorphological condition, considered as reference condition. The loss of hydrogeomorphological quality was closely linked to sociodemographical pressure, due to artificial elements in the river course, floodplain and land use changes on the basin. The assessment done based on pressures and impacts allowed the proposal of specific restoration objectives which facilitated the identification of the elements that degrade the hydrogeomorphological quality of the reaches, and helped the identification of specific restoration actions. In addition it was possible to set the reaches with the potentiality of being restored, those reversible and those that due to its high degradation were considered irreversible, and therefore not able to be restored, except for some rehabilitation or mitigation measures. The application in two basins, Oria and Oiartzun, concluded that 36% of the reaches could recover their geomorphological good status and a 40% could be considered as reference condition for other reaches. This geomorphological based reference condition definition could be linked and complete with ecological data.

A comparison of age, size, and fecundity of harvested and reference White Sucker populations

USGS Publications Warehouse

Begley, Meg; Coghlan, Stephen M.; Zydlewski, Joseph D.

2017-01-01

White Suckers Catostomus commersonii are an important source of fresh bait for the Maine lobster fishery. The Maine Department of Inland Fisheries and Wildlife began issuing commercial harvest permits in 1991, without reporting requirements or limits on the number of permits. There is recent concern that overfishing may be occurring. To infer impact, we investigated demographic differences between White Sucker populations in lakes open to harvest and those in lakes closed to harvest. Each of three harvested lakes was paired to a nearby closed lake as a reference based on general size, morphometry, and information on harvest pressure. In total, 976 spawning White Suckers were collected from the six lakes in 2014 (120–282 individuals/lake). Fish size, estimated age, fecundity, and mortality rates were compared between lakes. We hypothesized that we would find smaller, younger, and less-fecund individuals in harvested lakes compared to reference lakes. Size and age distributions for both sexes differed between nearly all lake pairs (except between males from one pair). White Suckers from reference lakes were larger and older and had greater gonadosomatic indices and fecundity than fish from harvested lakes. Estimated annual mortality rates were at least twofold higher in harvested lakes than in reference lakes. We detected some differences in von Bertalanffy growth parameters between lake pairs, as might occur under selective harvest pressure. The growth coefficient was smaller for reference lakes than for harvested lakes, while asymptotic length was greater for reference lakes than for harvested lakes. The data suggest that current levels of exploitation are resulting in greater age truncation in existing White Sucker populations.

Choice of Reference Serum Creatinine in Defining Acute Kidney Injury.

PubMed

Siew, Edward D; Matheny, Michael E

2015-01-01

The study of acute kidney injury (AKI) has expanded with the increasing availability of electronic health records and the use of standardized definitions. Understanding the impact of AKI between settings is limited by heterogeneity in the selection of reference creatinine to anchor the definition of AKI. In this mini-review, we discuss different approaches used to select reference creatinine and their relative merits and limitations. We reviewed the literature to obtain representative examples of published baseline creatinine definitions when pre-hospital data were not available, as well as literature evaluating the estimation of baseline renal function, using PubMed and reference back-tracing within known works. (1) Pre-hospital creatinine values are useful in determining reference creatinine, and in high-risk populations, the mean outpatient serum creatinine value 7-365 days before hospitalization closely approximates nephrology adjudication, (2) in patients without pre-hospital data, the eGFR 75 approach does not reliably estimate true AKI incidence in most at-risk populations, (3) using the lowest inpatient serum creatinine may be reasonable, especially in those with preserved kidney function, but may generously estimate AKI incidence and severity and miss community-acquired AKI that does not fully resolve, (4) using more specific definitions of AKI (e.g., KIDGO stages 2 and 3) may help to reduce the effects of misclassification when using surrogate values and (5) leveraging available clinical data may help refine the estimate of reference creatinine. Choosing reference creatinine for AKI calculation is important for AKI classification and study interpretation. We recommend obtaining data on pre-hospital kidney function, wherever possible. In studies where surrogate estimates are used, transparency in how they are applied and discussion that informs the reader of potential biases should be provided. Further work to refine the estimation of reference creatinine is needed. © 2015 S. Karger AG, Basel.

SIMPLIFIED PRACTICAL TEST METHOD FOR PORTABLE DOSE METERS USING SEVERAL SEALED RADIOACTIVE SOURCES.

PubMed

Mikamoto, Takahiro; Yamada, Takahiro; Kurosawa, Tadahiro

2016-09-01

Sealed radioactive sources which have small activity were employed for the determination of response and tests for non-linearity and energy dependence of detector responses. Close source-to-detector geometry (at 0.3 m or less) was employed to practical tests for portable dose meters to accumulate statistically sufficient ionizing currents. Difference between response in the present experimentally studied field and in the reference field complied with ISO 4037 due to non-uniformity of radiation fluence at close geometry was corrected by use of Monte Carlo simulation. As a consequence, corrected results were consistent with the results obtained in the ISO 4037 reference field within their uncertainties. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Stent-Grafts in the Management of Hemorrhagic Complications Related to Hemostatic Closure Devices: Report of Two Cases

DOE Office of Scientific and Technical Information (OSTI.GOV)

Giansante Abud, Daniel; Mounayer, Charbel; Saint-Maurice, Jean Pierre

We report 2 cases of hemorrhagic complications related to use of the Angio-Seal hemostatic closure device that were successfully managed with stent-grafts. Two patients with subarachnoid hemorrhage were referred to our departments for endovascular treatment of ruptured intracranial aneurysms. The treatment was performed through a femoral access; the sheaths were removed immediately after the procedures, and the punctures sites closed by Angio-Seals. Both patients presented clinical signs of hypovolemic shock after treatment. The diagnosis of active bleeding through the puncture site was made by emergency digital subtraction angiography. The lesions were managed with stent-grafts. The use of stent-grafts proved tomore » be efficient in the management of these life-threatening hemorrhagic complications following the use of the Angio-Seal hemostatic closure device.« less

Two new organic reference materials for δ13C and δ15N measurements and a new value for the δ13C of NBS 22 oil

USGS Publications Warehouse

Qi, Haiping; Coplen, Tyler B.; Geilmann, Heike; Brand, Willi A.; Böhlke, J.K.

2003-01-01

Analytical grade L-glutamic acid is chemically stable and has a C/N mole ratio of 5, which is close to that of many of natural biological materials, such as blood and animal tissue. Two L-glutamic acid reference materials with substantially different 13C and 15N abundances have been prepared for use as organic reference materials for C and N isotopic measurements. USGS40 is analytical grade L-glutamic acid and has a δ13C value of −26.24‰ relative to VPDB and a δ15N value of −4.52‰ relative to N2 in air. USGS41 was prepared by dissolving analytical grade L-glutamic acid with L-glutamic acid enriched in 13C and 15N. USGS41 has a δ13C value of +37.76‰ and a δ15N value of +47.57‰. The δ13C and δ15N values of both materials were measured against the international reference materials NBS 19 calcium carbonate (δ13C = +1.95‰), L-SVEC lithium carbonate (δ13C = −46.48‰), IAEA-N-1 ammonium sulfate (δ15N = 0.43‰), and USGS32 potassium nitrate (δ15N = 180‰) by on-line combustion continuous-flow and off-line dual-inlet isotope-ratio mass spectrometry. Both USGS40 and USGS41 are isotopically homogeneous; reproducibility of δ13C is better than 0.13‰, and that of δ15N is better than 0.13‰ in 100-μg amounts. These two isotopic reference materials can be used for (i) calibrating local laboratory reference materials, and (ii) quantifying drift with time, mass-dependent fractionations, and isotope-ratio-scale contraction in the isotopic analysis of various biological materials. Isotopic results presented in this paper yield a δ13C value for NBS 22 oil of −29.91‰, in contrast to the commonly accepted value of −29.78‰ for which off-line blank corrections probably have not been quantified satisfactorily.

Effect of twist and camber on the low-speed aerodynamic characteristics of a powered close-coupled wing-canard configuration

NASA Technical Reports Server (NTRS)

Paulson, J. W., Jr.; Thomas, J. L.

1978-01-01

A series of wind-tunnel tests were conducted in a V/STOL tunnel to determine the low-speed longitudinal aerodynamic characteristics of a powered close-coupled wing/canard fighter configuration. The data was obtained for a high angle-of-attack maneuvering configuration and a takeoff and landing configuration. The data presented in tabulated form are intended for reference purposes.

Optimal Control of a Circular Satellite Formation Subject to Gravitational Perturbations

DTIC Science & Technology

2007-03-01

fundamental reference in the study of the dynamics of close-proximity spacecraft is the paper by Clohessy and Wiltshire (5). In this work, the linear...dynamics for a satellite rendezvous problem are derived, which are now commonly known as either the Clohessy - Wiltshire (CW) equations or Hill’s...themselves to closed-form solutions, as did the Clohessy - Wiltshire development. When the nonlinear approach is undertaken, the numeric integration

Reference curve for the first-year growth response to growth hormone treatment in prepubertal children with idiopathic growth hormone deficiency: validation of the KIGS first-year growth response curve using the Belgian Register for the Study of Growth and Puberty Problems.

PubMed

Straetemans, Saartje; Roelants, Mathieu; Thomas, Muriel; Rooman, Raoul; De Schepper, Jean

2014-01-01

Comparing observed and expected growth after first-year growth hormone (GH) therapy is useful for identifying a poor growth response to GH. To generate a first-year, age-specific growth response reference curve for prepubertal Belgian children with idiopathic growth hormone deficiency (iGHD) treated with a standard weight-adjusted GH dose and to compare this national reference with the response references derived from KIGS. First-year height data of 357 prepubertal children (240 males) with iGHD were analyzed. Smooth reference curves of first-year height velocity (HV) in relation to age were created. Differences with the KIGS targets were evaluated after z-score transformation. The observed first-year HVs were log-normal distributed by age and decreased significantly with age (p<0.001). No GH dose or gender effect was observed (p=0.5). Distance to target height, severity of GHD and occurrence of multiple pituitary hormone deficiencies had a positive effect (p<0.01) on the calculated HV SDS. When applying the KIGS targets for severe iGHD, mean HV SDS was close to zero (-0.09±0.84). The developed age-specific growth response curves enable rapid identification of poor response to first-year GH treatment in prepubertal iGHD children. Our results validate the published growth targets derived from the KIGS database. © 2014 S. Karger AG, Basel.

Genetics Home Reference: schizophrenia

MedlinePlus

... Share: Email Facebook Twitter Home Health Conditions Schizophrenia Schizophrenia Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Schizophrenia is a brain disorder classified as a psychosis, ...

Fire Sale Loophole Closing Act

THOMAS, 113th Congress

Rep. Meng, Grace [D-NY-6

2013-01-14

House - 01/25/2013 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, And Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Fire Sale Loophole Closing Act

THOMAS, 113th Congress

Rep. Cicilline, David N. [D-RI-1

2013-01-03

House - 01/25/2013 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, And Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Detecting of the processes of the diamond formation using the monomineral thermobarometry .

NASA Astrophysics Data System (ADS)

Ashchepkov, Igor; Afanasiev, Valentin; Pokhilenko, Lyudmila; Logvinova, Alla; Vladykin, Nikolai

2010-05-01

The methods of the monomineral thermobarometry used for the reconstruction of the mantle sections beneath the kimberlite pipes (Ashchepkov et al., 2009) allow to determined PT range for the diamond inclusions (DI) and diamond bearing associations. They show various conditions for the crystallization of diamond for in mantle lithosphere beneath the Yakutia, Africa, and North America. In Yakutia most DI (Sobolev ea 1997, 2004; Logvinova ea., 2005 and ref their in) (Cr-pyropes, Mg -opx) form Mir and Udachnaya pipes are referred to the cold geotherms 35 (partly 33 mvm-2) at the pressure range from 35 to 80 kbar. Cr- pyropes (Ti-bearing) partly drops the on the heated area near convective branches 40-45 mvm-2 convective geotherms. Most Cr- rich pyroxenes refer to the coldest or heated (metasomatic type) at the deeper parts of the mantle columns while mildly Cr-rich varieties refer to the conditions of the crystallization from the melts related to the protokimberlites and associated carbonatites near the Graphite-Diamond boundary (G-D). They are more widely distributed in mantle beneath the Mir pipe where the essential part of mantle column from 50 to 35kbar was subjected to the refertilization. But chromite PT estimates mostly refer the heated conditions of the convective branch at the lithosphere base (~70-60kbar). They are most typical for the Alakite pipes. Diamond bearing eclogites show the some separate levels of crystallization with the high T-range reflecting conditions 35 to 45 mvm-2 mostly in the 60-50 kbar interval. They coincide with the levels of the intensive heating in the mantle columns. For the South Africa in the Mesozoic pipes beneath Lesotho - Jagersfontein (Viljoen ea. 2005), Finsch (Appleyard ea., 2004; Gurney, Switzer, 1973; She ea., 1983), Koffiefontein (Rickard ea., 1986), diamond bearing associations refer to three geotherm branches. The coldest (Cr-pyropes and Mg-Opx) is related to ancient subduction with the heating at 60 and 75 kbars. The 40 mvm-2 is related to the Diam-eclogites (To according to Krogh, 1988), Fe- Opx and chromites. And hottest (45mvm-2) refers to the magmatic type eclogites (cumulates) and some HT chromites (restites). In mantle columns beneath the Proterozoic pipes like Roberts Victor (Souter, Harte, 1988; Jacob ea, 2004), Premier (Gurney ea., 1985; Viljoin ea, 2009) the eclogitic DI trace mostly 40 mvm-2 geotherm but large amount of PT point drops onto advective hottest branch. Mostly eclogites are separated to several branches according to Mg#. The Fe- rich (ancient trondjemitic cumulates) are commonly low-To (LT). Conditions for the mantle beneath Tanzania (Stachel ea, 1998) and Ghana (Stachel ea, 1997) are close to 40mvm-2 geotherm from the deepest~80 kbar level to 35 kbar. The PT estimates for DI from Guinea (Denies, Harris, 2004), those from Angola kimberlites refer to colder geotherm branch. Similar but hotter conditions are detected for the V-Cm kimberlites from Botswana - Venetia (Viljoen ea 2009; Hin et al., 2009) which in mantle columns reveals subadiabatic branch from 1450oC (45mvm-2) but the Late Mesozoic pipes in this region like Letlhakane (Achtenbergh ea, 2001; Stienfenhofer ea, 1997; Deines, Harris, 2004), Orapa (Denies ea., 2009; Stachel ea., 2004) reveal conditions close to 42 mvm-2. PT for mantle xenoliths from the "of craton" Namibian pipes (Louwrensia, Hanous) (Mitchel, 1984; Boyd ea., 2004) trace the G-D boundary or are plotted above it and only relic associations are correspondent to the levels ~65-70 kbar. The PT for OPx and some Cpx in diamonds (Harris ea, 2004) from placers are close to this boundary but those for hot (1400oC) eclogites (magmatic type) are correspondent to the lower levels of mantle columns or coincides with the hottest PT conditions for peridotites. The location of the PT estimate for DI above the Diam -Graph boundary is probably due to metastable crystallization or mantle diapirism. In North America kimberlites the conditions for the DI are mostly LT. Garnet DI from the Diavic mine (Schultze ea, 2008) locate from 50 to 70 kbar near 36 mvm-2 geotherm. Hi-Mg eclogites demonstrate more LT conditions, while the conditions for Hi-Fe eclogite are close to convective 40 mvm-2 branch similar to those for Chromite DI and several points are lose to 45 mvm-2or locate slightly above Diam-Graph. DI from Panda (Tomlinson ea, 2006; Greighton ea, 2009) kimberlites from the same region reveals close PT but are slightly LT. The PT conditions for diamondiferous eclogites from Jericho (Lac De Gras) (Heaman ea, 2004) are most LT in Slave craton (33 -36 mvm-2) at 50-65 kbar range. The eclogitic Cpx and peridotitic pyrope DI (Aulbach et al., 2004) from Buffalo Heard (Banas ea., 2006) at the west of region also trace 36 heating to 40 mvm-2 geotherm at 70 kbar and some more hot PT points trace D-G line. In Wayoming craton the Cr-pyrope DI from low Paleozoic KL-1 pipe (Coopersmith ea ., 2004; Schultze ea 2008) reflect the range 35-40 mvm-2 of heating at lithosphere base 75-65 kbar and more Fe-rich Opx demonstrate HT conditions probably refer to cumulates. Thus the mantle inclusion in different part of the Earth mantle in general repeats the conditions of the whole mantle column. They reflect higher PT gradients in ancient time. In Africa they are hotter in general and often trace advective branches. While in the thicker and colder lithosphere beneath the Slave craton DI reveal colder and deeper in general conditions. In Siberia many of DI especially Chr as well as Diam- eclogites reflect the conditions of the heating and the influence of the protokimberlite melts.

Non-Invasive Monitoring of Cardiac Output in Critical Care Medicine.

PubMed

Nguyen, Lee S; Squara, Pierre

2017-01-01

Critically ill patients require close hemodynamic monitoring to titrate treatment on a regular basis. It allows administering fluid with parsimony and adjusting inotropes and vasoactive drugs when necessary. Although invasive monitoring is considered as the reference method, non-invasive monitoring presents the obvious advantage of being associated with fewer complications, at the expanse of accuracy, precision, and step-response change. A great many methods and devices are now used over the world, and this article focuses on several of them, providing with a brief review of related underlying physical principles and validation articles analysis. Reviewed methods include electrical bioimpedance and bioreactance, respiratory-derived cardiac output (CO) monitoring technique, pulse wave transit time, ultrasound CO monitoring, multimodal algorithmic estimation, and inductance thoracocardiography. Quality criteria with which devices were reviewed included: accuracy (closeness of agreement between a measurement value and a true value of the measured), precision (closeness of agreement between replicate measurements on the same or similar objects under specified conditions), and step response change (delay between physiological change and its indication). Our conclusion is that the offer of non-invasive monitoring has improved in the past few years, even though further developments are needed to provide clinicians with sufficiently accurate devices for routine use, as alternative to invasive monitoring devices.

Typhus-like Fevers of Unknown Ætiology, with Special Reference to the Malay States

PubMed Central

Fletcher, William

1930-01-01

Typhus exanthematicus, Rocky Mountain fever, and the tsutsugamushi disease have been classified in the “typhus group” by Megaw, as louse-typhus, tick-typhus, and mite-typhus. He has added a fourth-class, comprising typhus-like fevers, with unknown vectors. It is the diseases of this class with which this paper is concerned. Endemic typhus (Brill's disease) is very closely related to typhus fever; the Weil-Felix reaction is positive, typhus-like vascular lesions are present, and there is cross-immunity with typhus. In the exanthematic fever of Marseilles the relationship is more superficial; there is neither cross-immunity nor vascular lesion, and the Weil-Felix reaction is negative. Some, e.g., the scrub-typhus of Malaya (vector probably a mite), are more nearly related to tsutsugamushi than to typhus; others, e.g., Indian “tick-typhus” (vector probably a tick), to Rocky Mountain fever. All are non-contagious, non-epidemic, warm-weather diseases. They are unassociated with dirt, squalor, or lice, and are restricted to definite foci. Probably rodents or other animals are the reservoirs of the virus. On the question of identity with typhus, health authorities decide that notification is unnecessary; typhus introduced into America spreads, Brill's disease does not. These typhus-like diseases are not the same in all the countries where they occur. There are two main groups: (1) an urban group, more closely related to typhus, in which the Weil-Felix reaction is positive; (2) a rural group, more closely related to tsutsugamushi and Rocky Mountain fever, in which the Weil-Felix reaction is negative. There is a special non-indologenic strain of B. proteus, which is agglutinated in some of the fevers belonging to the second group. Tropical typhus in the Malay States: (1) urban form, or “shop-typhus,” resembling Brill's disease; (2) rural form or “scrub-typhus.” Peculiar association with oil-palms and coarse grass. PMID:19987538

[Non-invasive assessment of the perfusion of wounds using power Doppler imaging: vacuum assisted closure versus direct wound closure].

PubMed

Jungius, K P; Chilla, B K; Labler, L; Teodorovic, N; Marincek, B

2006-10-01

The goal of our study was to assess the perfusion in wounds treated by vacuum assisted closure (VAC) compared to primary wound closure. Power Doppler Ultrasound (PDUS) was carried out under standardised conditions in 15 VAC-treated and 10 primarily closed wounds as well as on altogether 25 intraindividual reference areas. All data were sent to a work station for post-processing to determine the perfused area. Statistical data analysis was performed with the Mann-Whitney test. Both VAC-treated wounds and primarily closed wounds showed a significant increase of the perfusion when compared to the intraindividual reference area (p < 0.0001). In VAC-treated wounds, a markedly increased perfusion was measured compared to the wounds closed primarily (p < 0.0001). Perfusion decreased during treatment, but in two VAC-treated wounds, an initial increase of the perfusion was observed. Both these wounds were grossly infected. PDUS allows the quantification of the differences in wound perfusion. This can be helpful in the detection of progressive local wound infections.

Modeling and closed-loop control of hypnosis by means of bispectral index (BIS) with isoflurane.

PubMed

Gentilini, A; Rossoni-Gerosa, M; Frei, C W; Wymann, R; Morari, M; Zbinden, A M; Schnider, T W

2001-08-01

A model-based closed-loop control system is presented to regulate hypnosis with the volatile anesthetic isoflurane. Hypnosis is assessed by means of the bispectral index (BIS), a processed parameter derived from the electroencephalogram. Isoflurane is administered through a closed-circuit respiratory system. The model for control was identified on a population of 20 healthy volunteers. It consists of three parts: a model for the respiratory system, a pharmacokinetic model and a pharmacodynamic model to predict BIS at the effect compartment. A cascaded internal model controller is employed. The master controller compares the actual BIS and the reference value set by the anesthesiologist and provides expired isoflurane concentration references to the slave controller. The slave controller maneuvers the fresh gas anesthetic concentration entering the respiratory system. The controller is designed to adapt to different respiratory conditions. Anti-windup measures protect against performance degradation in the event of saturation of the input signal. Fault detection schemes in the controller cope with BIS and expired concentration measurement artifacts. The results of clinical studies on humans are presented.

Genetics Home Reference: distal arthrogryposis type 1

MedlinePlus

... Conditions Distal arthrogryposis type 1 Distal arthrogryposis type 1 Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Distal arthrogryposis type 1 is a disorder ...

Genetics Home Reference: lymphangioleiomyomatosis

MedlinePlus

... Genetics Share: Email Facebook Twitter Home Health Conditions LAM Lymphangioleiomyomatosis Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Lymphangioleiomyomatosis ( LAM ) is a condition that affects the lungs , the ...

76 FR 24923 - National Science Board; Sunshine Act Meetings; Notice

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-03

...: Some portions open, some portions closed. UPDATES: Please refer to the National Science Board Web site... Information Item: Status Deep Underground Science and Engineering Laboratory Information Item: High...

Can natural variability trigger effects on fish and fish habitat as defined in environment Canada's metal mining environmental effects monitoring program?

PubMed

Mackey, Robin; Rees, Cassandra; Wells, Kelly; Pham, Samantha; England, Kent

2013-01-01

The Metal Mining Effluent Regulations (MMER) took effect in 2002 and require most metal mining operations in Canada to complete environmental effects monitoring (EEM) programs. An "effect" under the MMER EEM program is considered any positive or negative statistically significant difference in fish population, fish usability, or benthic invertebrate community EEM-defined endpoints. Two consecutive studies with the same statistically significant differences trigger more intensive monitoring, including the characterization of extent and magnitude and investigation of cause. Standard EEM study designs do not require multiple reference areas or preexposure sampling, thus results and conclusions about mine effects are highly contingent on the selection of a near perfect reference area and are at risk of falsely labeling natural variation as mine related "effects." A case study was completed to characterize the natural variability in EEM-defined endpoints during preexposure or baseline conditions. This involved completing a typical EEM study in future reference and exposure lakes surrounding a proposed uranium (U) mine in northern Saskatchewan, Canada. Moon Lake was sampled as the future exposure area as it is currently proposed to receive effluent from the U mine. Two reference areas were used: Slush Lake for both the fish population and benthic invertebrate community surveys and Lake C as a second reference area for the benthic invertebrate community survey. Moon Lake, Slush Lake, and Lake C are located in the same drainage basin in close proximity to one another. All 3 lakes contained similar water quality, fish communities, aquatic habitat, and a sediment composition largely comprised of fine-textured particles. The fish population survey consisted of a nonlethal northern pike (Esox lucius) and a lethal yellow perch (Perca flavescens) survey. A comparison of the 5 benthic invertebrate community effect endpoints, 4 nonlethal northern pike population effect endpoints, and 10 lethal yellow perch effect endpoints resulted in the observation of several statistically significant differences at the future exposure area relative to the reference area and/or areas. When the data from 2 reference areas assessed for the benthic invertebrate community survey were pooled, no significant differences in effect endpoints were observed. These results demonstrate weaknesses in the definition of an "effect" used by the MMER EEM program and in the use of a single reference area. Determination of the ecological significance of statistical differences identified as part of EEM programs conducted during the operational period should consider preexisting (background) natural variability between reference and exposure areas. Copyright © 2012 SETAC.

Testosterone, Cortisol and Financial Risk-Taking

PubMed Central

Herbert, Joe

2018-01-01

Both testosterone and cortisol have major actions on financial decision-making closely related to their primary biological functions, reproductive success and response to stress, respectively. Financial risk-taking represents a particular example of strategic decisions made in the context of choice under conditions of uncertainty. Such decisions have multiple components, and this article considers how much we know of how either hormone affects risk-appetite, reward value, information processing and estimation of the costs and benefits of potential success or failure, both personal and social. It also considers how far we can map these actions on neural mechanisms underlying risk appetite and decision-making, with particular reference to areas of the brain concerned in either cognitive or emotional functions. PMID:29867399

Case studies in configuration control for redundant robots

NASA Technical Reports Server (NTRS)

Seraji, H.; Lee, T.; Colbaugh, R.; Glass, K.

1989-01-01

A simple approach to configuration control of redundant robots is presented. The redundancy is utilized to control the robot configuration directly in task space, where the task will be performed. A number of task-related kinematic functions are defined and combined with the end-effector coordinates to form a set of configuration variables. An adaptive control scheme is then utilized to ensure that the configuration variables track the desired reference trajectories as closely as possible. Simulation results are presented to illustrate the control scheme. The scheme has also been implemented for direct online control of a PUMA industrial robot, and experimental results are presented. The simulation and experimental results validate the configuration control scheme for performing various realistic tasks.

The electronic spectra of benzo[b]thiete and transient o-thiobenzoquinonemethide. Spectral assignments on the basis of the electronic spectra of aniline, thiophenol, thioanisole, all-trans-octatetraene and transient o-xylylene in conjunction with quantum-chemical calculations

NASA Astrophysics Data System (ADS)

Schweig, Armin; Diehl, Frank; Kesper, Karl; Meyer, Hermann

1989-07-01

The electronic absorption spectra of benzo[b]thiete ( 1) and of transient o-thiobenzoquinonemethide ( 2) have been obtained. Semiempirical valence-electron calculations using the CNDO/S SECI, CNDO/S PERTCI and LNDO/S PERTCI methods and correlation diagrams using suitable reference compounds ad aniline, thiophenol, thioanisole, all-trans-octatetraene and o-xylylene are applied to the interpretation of the spectra. The results clearly reveal 1 as a typically donor-substituted benzene derivative and 2 as a polyene-like system closely related to o-xylylene.

Reemergence of rabies in the southern Han river region, Korea.

PubMed

Oem, Jae-Ku; Kim, Seong-Hee; Kim, Yeon-Hee; Lee, Myoung-Heon; Lee, Kyoung-Ki

2014-07-01

Recently, 11 cases of animal rabies were reported in the southern region (Suwon and Hwaseong cities) of Gyeonggi Province, South Korea. The cases were temporally separated into two cases in dogs (Canis lupus familiaris) in spring 2012 and nine cases in domestic animals and wildlife in winter 2012-13. All carcasses were submitted for histopathologic examination and viral antigen identification. Sequences of the glycoprotein, nucleoprotein, and glycoprotein-large polymerase protein intergenic noncoding loci of the 11 strains were determined and compared with published reference sequences. All rabies strains were closely related to the Gangwon strains isolated in 2008-09, suggesting that the rabies virus strains isolated in Gyeonggi were introduced from Gangwon Province.

[Intervening factors in attention flow of professionals injured by biological material].

PubMed

Ribeiro, Luana Cássia Miranda; Souza, Adenícia Custódia Silva E; Tipple, Anaclara Ferreira Veiga; Melo, Dulcelene Sousa; Peixoto, Myrian Karla Ayres Veronez; Munari, Denize Bouttelet

2014-06-01

To describe the barriers and facilitator factors to follow the attention flow of professionals injured by biological material in the worker perspective. Qualitative descriptive study with data collected through individual interviews with 18 injured workers, assisted in reference public units in the city of Goiânia. The content analysis was carried out with assistance of the ATLAS.ti 6.2 software, under the work organization and subjective perspectives. From the interviews regarding the barriers and facilitator factors emerged the categories: organizational structure, Support from close people, and Knowledge influence. The organized services have enabled more qualified consultations and the workers follow-up, which caused a satisfaction feeling in relation to the working environment.

Evaluation of the Liberian Petroleum Refining Company operations: crude oil refining vs product importation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Samuels, G.; Barron, W.F.; Barnes, R.W.

1985-02-01

This report is one of a series of project papers providing background information for an assessment of energy options for Liberia, West Africa. It presents information on a controversial recommendation of the energy assessment - that the only refinery in the country be closed and refined products be imported for a savings of approximately $20 million per year. The report reviews refinery operations, discusses a number of related issues, and presents a detailed analysis of the economics of the refinery operations as of 1982. This analysis corroborates the initial estimate of savings to be gained from importing all refined products.more » 1 reference, 24 tables.« less

Muon tomography imaging algorithms for nuclear threat detection inside large volume containers with the Muon Portal detector

NASA Astrophysics Data System (ADS)

Riggi, S.; Antonuccio-Delogu, V.; Bandieramonte, M.; Becciani, U.; Costa, A.; La Rocca, P.; Massimino, P.; Petta, C.; Pistagna, C.; Riggi, F.; Sciacca, E.; Vitello, F.

2013-11-01

Muon tomographic visualization techniques try to reconstruct a 3D image as close as possible to the real localization of the objects being probed. Statistical algorithms under test for the reconstruction of muon tomographic images in the Muon Portal Project are discussed here. Autocorrelation analysis and clustering algorithms have been employed within the context of methods based on the Point Of Closest Approach (POCA) reconstruction tool. An iterative method based on the log-likelihood approach was also implemented. Relative merits of all such methods are discussed, with reference to full GEANT4 simulations of different scenarios, incorporating medium and high-Z objects inside a container.

Schwannomatosis of Cervical Vagus Nerve.

PubMed

Abdulla, Faheem Ahmed; Sasi, M P

2016-01-01

Cervical vagal schwannoma is a rare entity among lesions presenting as a neck mass. They are usually slow-growing benign lesions closely associated with the vagus nerve. They are usually solitary and asymptomatic. Multiple schwannomas occurring in patients without neurofibromatosis (NF) are rare and have recently been referred to as schwannomatosis. Here, we present a case of a neck mass that had imaging features suggestive of vagal schwannoma and was operated upon. Intraoperatively, it was discovered to be a case of multiple vagal cervical schwannoma, all directly related to the right vagus nerve, and could be resected from the nerve in toto preserving the function of the vagus nerve. Final HPR confirmed our pre-op suspicion of vagal schwannomatosis.

Schwannomatosis of Cervical Vagus Nerve

PubMed Central

Sasi, M. P.

2016-01-01

Cervical vagal schwannoma is a rare entity among lesions presenting as a neck mass. They are usually slow-growing benign lesions closely associated with the vagus nerve. They are usually solitary and asymptomatic. Multiple schwannomas occurring in patients without neurofibromatosis (NF) are rare and have recently been referred to as schwannomatosis. Here, we present a case of a neck mass that had imaging features suggestive of vagal schwannoma and was operated upon. Intraoperatively, it was discovered to be a case of multiple vagal cervical schwannoma, all directly related to the right vagus nerve, and could be resected from the nerve in toto preserving the function of the vagus nerve. Final HPR confirmed our pre-op suspicion of vagal schwannomatosis. PMID:27807496

Near-optimal reconfiguration and maintenance of close spacecraft formations.

PubMed

Lovell, T A; Tragesser, S G

2004-05-01

This paper investigates orbit guidance algorithms for formation flying experiments. The relative motion of one satellite about a reference satellite is formulated in terms of a set of parameters that clearly describe the size, shape, and orientation of the formation. A nominal three-impulse burn maneuver algorithm is presented that is applicable for both reconfiguration and maintenance of spacecraft formations. Two methods of implementing the algorithm are discussed, one involving fixed times between each burn and one allowing the wait times to vary. The implications of employing four or more impulses for maneuvers are assessed. Examples applying the algorithm to various formation scenarios are presented, along with practical implications of each result.

Frequency comparison involving the Romanian primary length standard RO.1 within the framework of the EUROMET Project #498

NASA Astrophysics Data System (ADS)

Popescu, Gheorghe

2001-06-01

An international frequency comparison was carried out at the Bundesamt fuer Eich- und Vermessungswessen (BEV), Vienna, within the framework of the EUROMET Project #498 from August 29 to September 5, 1999. The frequency differences obtained when the RO.1 laser from the National Institute for Laser, Plasma and Radiation Physics (NILPRP), Romania, was compared with five lasers from Austria (BEV1), Czech Republic (PLD1), France (BIPM3), Poland (GUM1) and Hungary (OMH1) are reported. Frequency differences were computed by using the matrix determinations for the group d, e, f, g. Considering the frequency differences measured for a group of three lasers compared to each other, we call the closing frequency the difference between measured and expected frequency difference (resulting from the previous two measurements). For the RO1 laser, when the BIPM3 laser was the reference laser, the closing frequencies range from +8.1 kHz to - 3.8 kHz. The relative Allan standard deviation was used to express the frequency stability and resulted 3.8 parts in 1012 for 100 s sampling time and 14000 s duration of the measurements. The averaged offset frequency relative to the BIPM4 stationary laser was 5.6 kHz and the standard deviation was 9.9 kHz.

DNA Barcoding analysis of seafood accuracy in Washington, D.C. restaurants

PubMed Central

Stern, David B.; Castro Nallar, Eduardo; Rathod, Jason

2017-01-01

In Washington D.C., recent legislation authorizes citizens to test if products are properly represented and, if they are not, to bring a lawsuit for the benefit of the general public. Recent studies revealing the widespread phenomenon of seafood substitution across the United States make it a fertile area for consumer protection testing. DNA barcoding provides an accurate and cost-effective way to perform these tests, especially when tissue alone is available making species identification based on morphology impossible. In this study, we sequenced the 5′ barcoding region of the Cytochrome Oxidase I gene for 12 samples of vertebrate and invertebrate food items across six restaurants in Washington, D.C. and used multiple analytical methods to make identifications. These samples included several ambiguous menu listings, sequences with little genetic variation among closely related species and one sequence with no available reference sequence. Despite these challenges, we were able to make identifications for all samples and found that 33% were potentially mislabeled. While we found a high degree of mislabeling, the errors involved closely related species and we did not identify egregious substitutions as have been found in other cities. This study highlights the efficacy of DNA barcoding and robust analyses in identifying seafood items for consumer protection. PMID:28462038

Mansonella ozzardi mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding.

PubMed

Crainey, James Lee; Marín, Michel Abanto; Silva, Túllio Romão Ribeiro da; de Medeiros, Jansen Fernandes; Pessoa, Felipe Arley Costa; Santos, Yago Vinícius; Vicente, Ana Carolina Paulo; Luz, Sérgio Luiz Bessa

2018-04-18

Despite the broad distribution of M. ozzardi in Latin America and the Caribbean, there is still very little DNA sequence data available to study this neglected parasite's epidemiology. Mitochondrial DNA (mtDNA) sequences, especially the cytochrome oxidase (CO1) gene's barcoding region, have been targeted successfully for filarial diagnostics and for epidemiological, ecological and evolutionary studies. MtDNA-based studies can, however, be compromised by unrecognised mitochondrial pseudogenes, such as Numts. Here, we have used shot-gun Illumina-HiSeq sequencing to recover the first complete Mansonella genus mitogenome and to identify several mitochondrial-origin pseudogenes. Mitogenome phylogenetic analysis placed M. ozzardi in the Onchocercidae "ONC5" clade and suggested that Mansonella parasites are more closely related to Wuchereria and Brugia genera parasites than they are to Loa genus parasites. DNA sequence alignments, BLAST searches and conceptual translations have been used to compliment phylogenetic analysis showing that M. ozzardi from the Amazon and Caribbean regions are near-identical and that previously reported Peruvian M. ozzardi CO1 reference sequences are probably of pseudogene origin. In addition to adding a much-needed resource to the Mansonella genus's molecular tool-kit and providing evidence that some M. ozzardi CO1 sequence deposits are pseudogenes, our results suggest that all Neotropical M. ozzardi parasites are closely related.

The Effect of Electroencephalogram (EEG) Reference Choice on Information-Theoretic Measures of the Complexity and Integration of EEG Signals

PubMed Central

Trujillo, Logan T.; Stanfield, Candice T.; Vela, Ruben D.

2017-01-01

Converging evidence suggests that human cognition and behavior emerge from functional brain networks interacting on local and global scales. We investigated two information-theoretic measures of functional brain segregation and integration—interaction complexity CI(X), and integration I(X)—as applied to electroencephalographic (EEG) signals and how these measures are affected by choice of EEG reference. CI(X) is a statistical measure of the system entropy accounted for by interactions among its elements, whereas I(X) indexes the overall deviation from statistical independence of the individual elements of a system. We recorded 72 channels of scalp EEG from human participants who sat in a wakeful resting state (interleaved counterbalanced eyes-open and eyes-closed blocks). CI(X) and I(X) of the EEG signals were computed using four different EEG references: linked-mastoids (LM) reference, average (AVG) reference, a Laplacian (LAP) “reference-free” transformation, and an infinity (INF) reference estimated via the Reference Electrode Standardization Technique (REST). Fourier-based power spectral density (PSD), a standard measure of resting state activity, was computed for comparison and as a check of data integrity and quality. We also performed dipole source modeling in order to assess the accuracy of neural source CI(X) and I(X) estimates obtained from scalp-level EEG signals. CI(X) was largest for the LAP transformation, smallest for the LM reference, and at intermediate values for the AVG and INF references. I(X) was smallest for the LAP transformation, largest for the LM reference, and at intermediate values for the AVG and INF references. Furthermore, across all references, CI(X) and I(X) reliably distinguished between resting-state conditions (larger values for eyes-open vs. eyes-closed). These findings occurred in the context of the overall expected pattern of resting state PSD. Dipole modeling showed that simulated scalp EEG-level CI(X) and I(X) reflected changes in underlying neural source dependencies, but only for higher levels of integration and with highest accuracy for the LAP transformation. Our observations suggest that the Laplacian-transformation should be preferred for the computation of scalp-level CI(X) and I(X) due to its positive impact on EEG signal quality and statistics, reduction of volume-conduction, and the higher accuracy this provides when estimating scalp-level EEG complexity and integration. PMID:28790884

Genetics Home Reference: congenital hypothyroidism

MedlinePlus

... Facebook Twitter Home Health Conditions Congenital hypothyroidism Congenital hypothyroidism Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Congenital hypothyroidism is a partial or complete loss of function ...

Gun Show Loophole Closing Act of 2013

THOMAS, 113th Congress

Rep. McCarthy, Carolyn [D-NY-4

2013-01-03

House - 01/25/2013 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, And Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Genetics Home Reference: leukocyte adhesion deficiency type 1

MedlinePlus

... adhesion deficiency type 1 Leukocyte adhesion deficiency type 1 Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Leukocyte adhesion deficiency type 1 is a ...

Genetics Home Reference: cap myopathy

MedlinePlus

... Email Facebook Twitter Home Health Conditions Cap myopathy Cap myopathy Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Cap myopathy is a disorder that primarily affects skeletal ...

Genetics Home Reference: cyclic neutropenia

MedlinePlus

... Facebook Twitter Home Health Conditions Cyclic neutropenia Cyclic neutropenia Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Cyclic neutropenia is a disorder that causes frequent infections and ...

Genetics Home Reference: clopidogrel resistance

MedlinePlus

... Email Facebook Twitter Home Health Conditions Clopidogrel resistance Clopidogrel resistance Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Clopidogrel resistance is a condition in which the drug ...

Genetics Home Reference: alpha thalassemia

MedlinePlus

... Facebook Twitter Home Health Conditions Alpha thalassemia Alpha thalassemia Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Alpha thalassemia is a blood disorder that reduces the production ...

Genetics Home Reference: beta thalassemia

MedlinePlus

... Facebook Twitter Home Health Conditions Beta thalassemia Beta thalassemia Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Beta thalassemia is a blood disorder that reduces the production ...

Tone as a health concept: An analysis.

PubMed

McDowall, Donald; Emmanuel, Elizabeth; Grace, Sandra; Chaseling, Marilyn

2017-11-01

Concept analysis. This paper is a report on the analysis of the concept of tone in chiropractic. The purpose of this paper is to clarify the concept of tone as originally understood by Daniel David Palmer from 1895 to 1914 and to monitor its evolution over time. Data was sourced from Palmer's original work, published between 1895 and 1914. A literature search from 1980 to 2016 was also performed on the online databases CINHAL, PubMed and Scopus with key terms including 'tone', 'chiropractic', 'Palmer', 'vitalism', 'health', 'homeostasis', 'holism' and 'wellness'. Finally hand-searches were conducted through chiropractic books and professional literature from 1906 to 1980 for any references to 'tone'. Rodgers' evolutionary method of analysis was used to categorise the data in relation to the surrogates, attributes, references, antecedents and consequences of tone. A total of 49 references were found: five from publications by Palmer; three from the database searches, and; the remaining 41 from professional books, trade journals and websites. There is no clear interpretation of tone in the contemporary chiropractic literature. Tone is closely aligned with functional neurology and can be understood as an interface between the metaphysical and the biomedical. Using the concept of tone as a foundation for practice could strengthen the identity of the chiropractic profession. Copyright © 2017 Elsevier Ltd. All rights reserved.

Epidermal electronics for electromyography: An application to swallowing therapy.

PubMed

Constantinescu, Gabriela; Jeong, Jae-Woong; Li, Xinda; Scott, Dylan K; Jang, Kyung-In; Chung, Hyun-Joong; Rogers, John A; Rieger, Jana

2016-08-01

Head and neck cancer treatment alters the anatomy and physiology of patients. Resulting swallowing difficulties can lead to serious health concerns. Surface electromyography (sEMG) is used as an adjuvant to swallowing therapy exercises. sEMG signal collected from the area under the chin provides visual biofeedback from muscle contractions and is used to help patients perform exercises correctly. However, conventional sEMG adhesive pads are relatively thick and difficult to effectively adhere to a patient's altered chin anatomy, potentially leading to poor signal acquisition in this population. Here, the emerging technology of epidermal electronics is introduced, where ultra-thin geometry allows for close contouring of the chin. The two objectives of this study were to (1) assess the potential of epidermal electronics technology for use with swallowing therapy and (2) assess the significance of the reference electrode placement. This study showed comparative signals between the new epidermal sEMG patch and the conventional adhesive patches used by clinicians. Furthermore, an integrated reference yielded optimal signal for clinical use; this configuration was more robust to head movements than when an external reference was used. Improvements for future iterations of epidermal sEMG patches specific to day-to-day clinical use are suggested. Copyright © 2016 IPEM. Published by Elsevier Ltd. All rights reserved.

Compact Closed Cycle Brayton System Feasibility Study. Volume II.

DTIC Science & Technology

1979-08-01

62 3.5.3 Feasibility with Respect to Other Considerations 3-69 3.6 References 3-89 4.0 DEVELOPMENT PROGRAM 4-1 4.1 Overall CCCBS 4-1 4.1.1 System... Development Program 4-59 1 4.4 References 4-63 5.0 APPLICATIONS OF CCCBS RESULTS 5-1 5.1 Applications 5-2 5.1.1 Advantages of CCCBS 5-2 m 5.1.2...52 9.4.6 Summary of Results 9-59 9.5 Recommended Materials Test Program for Development 9-59 of the CCCBS 9.6 References 9-62 vi I! I I FIGURES g Page

Hatch closing between Mir and Shuttle

NASA Image and Video Library

1996-03-28

S76-E-5222 (28 March 1996) --- One would take this triumvirate of thumbs-up symbols to refer to a successful hatch closing, as the Space Shuttle Atlantis is about to be separated from its link with Russia's Mir Space Station. Hold photo with frame number and clock at bottom left. Astronaut Kevin P. Chilton, mission commander, is at lower left. Others are astronauts Richard A. Searfoss (top), pilot, and Michael R. (Rich) Clifford, mission specialist.

Close of Service Workshop. Trainer Guidelines and Workshop Materials. Training for Development. Peace Corps Information Collection & Exchange Training Manual No. T-07.

ERIC Educational Resources Information Center

Peace Corps, Washington, DC. Information Collection and Exchange Div.

This manual is intended for use by Peace Corps staff members and/or trainers contracted to facilitate the close of service (COS) workshop, the purpose of which is to help volunteers make the transition from the host country back to the United States. Chapter 1 provides an overview of the COS workshop. Chapter 2 is a trainer reference. It helps the…

Hematology and biochemistry reference intervals for Ontario commercial nursing pigs close to the time of weaning

PubMed Central

Perri, Amanda M.; O’Sullivan, Terri L.; Harding, John C.S.; Wood, R. Darren; Friendship, Robert M.

2017-01-01

The evaluation of pig hematology and biochemistry parameters is rarely done largely due to the costs associated with laboratory testing and labor, and the limited availability of reference intervals needed for interpretation. Within-herd and between-herd biological variation of these values also make it difficult to establish reference intervals. Regardless, baseline reference intervals are important to aid veterinarians in the interpretation of blood parameters for the diagnosis and treatment of diseased swine. The objective of this research was to provide reference intervals for hematology and biochemistry parameters of 3-week-old commercial nursing piglets in Ontario. A total of 1032 pigs lacking clinical signs of disease from 20 swine farms were sampled for hematology and iron panel evaluation, with biochemistry analysis performed on a subset of 189 randomly selected pigs. The 95% reference interval, mean, median, range, and 90% confidence intervals were calculated for each parameter. PMID:28373729

Species identification of mutans streptococci by groESL gene sequence.

PubMed

Hung, Wei-Chung; Tsai, Jui-Chang; Hsueh, Po-Ren; Chia, Jean-San; Teng, Lee-Jene

2005-09-01

The near full-length sequences of the groESL genes were determined and analysed among eight reference strains (serotypes a to h) representing five species of mutans group streptococci. The groES sequences from these reference strains revealed that there are two lengths (285 and 288 bp) in the five species. The intergenic spacer between groES and groEL appears to be a unique marker for species, with a variable size (ranging from 111 to 310 bp) and sequence. Phylogenetic analysis of groES and groEL separated the eight serotypes into two major clusters. Strains of serotypes b, c, e and f were highly related and had groES gene sequences of the same length, 288 bp, while strains of serotypes a, d, g and h were also closely related and their groES gene sequence lengths were 285 bp. The groESL sequences in clinical isolates of three serotypes of S. mutans were analysed for intraspecies polymorphism. The results showed that the groESL sequences could provide information for differentiation among species, but were unable to distinguish serotypes of the same species. Based on the determined sequences, a PCR assay was developed that could differentiate members of the mutans streptococci by amplicon size and provide an alternative way for distinguishing mutans streptococci from other viridans streptococci.

Diffusivity anomaly in modified Stillinger-Weber liquids

NASA Astrophysics Data System (ADS)

Sengupta, Shiladitya; Vasisht, Vishwas V.; Sastry, Srikanth

2014-01-01

By modifying the tetrahedrality (the strength of the three body interactions) in the well-known Stillinger-Weber model for silicon, we study the diffusivity of a series of model liquids as a function of tetrahedrality and temperature at fixed pressure. Previous work has shown that at constant temperature, the diffusivity exhibits a maximum as a function of tetrahedrality, which we refer to as the diffusivity anomaly, in analogy with the well-known anomaly in water upon variation of pressure at constant temperature. We explore to what extent the structural and thermodynamic changes accompanying changes in the interaction potential can help rationalize the diffusivity anomaly, by employing the Rosenfeld relation between diffusivity and the excess entropy (over the ideal gas reference value), and the pair correlation entropy, which provides an approximation to the excess entropy in terms of the pair correlation function. We find that in the modified Stillinger-Weber liquids, the Rosenfeld relation works well above the melting temperatures but exhibits deviations below, with the deviations becoming smaller for smaller tetrahedrality. Further we find that both the excess entropy and the pair correlation entropy at constant temperature go through maxima as a function of the tetrahedrality, thus demonstrating the close relationship between structural, thermodynamic, and dynamical anomalies in the modified Stillinger-Weber liquids.

Comparison of five Lonicera flowers by simultaneous determination of multi-components with single reference standard method and principal component analysis.

PubMed

Gao, Wen; Wang, Rui; Li, Dan; Liu, Ke; Chen, Jun; Li, Hui-Jun; Xu, Xiaojun; Li, Ping; Yang, Hua

2016-01-05

The flowers of Lonicera japonica Thunb. were extensively used to treat many diseases. As the demands for L. japonica increased, some related Lonicera plants were often confused or misused. Caffeoylquinic acids were always regarded as chemical markers in the quality control of L. japonica, but they could be found in all Lonicera species. Thus, a simple and reliable method for the evaluation of different Lonicera flowers is necessary to be established. In this work a method based on single standard to determine multi-components (SSDMC) combined with principal component analysis (PCA) for control and distinguish of Lonicera species flowers have been developed. Six components including three caffeoylquinic acids and three iridoid glycosides were assayed simultaneously using chlorogenic acid as the reference standard. The credibility and feasibility of the SSDMC method were carefully validated and the results demonstrated that there were no remarkable differences compared with external standard method. Finally, a total of fifty-one batches covering five Lonicera species were analyzed and PCA was successfully applied to distinguish the Lonicera species. This strategy simplifies the processes in the quality control of multiple-componential herbal medicine which effectively adapted for improving the quality control of those herbs belonging to closely related species. Copyright © 2015 Elsevier B.V. All rights reserved.

Molecular and systems approaches towards drought-tolerant canola crops.

PubMed

Zhu, Mengmeng; Monroe, J Grey; Suhail, Yasir; Villiers, Florent; Mullen, Jack; Pater, Dianne; Hauser, Felix; Jeon, Byeong Wook; Bader, Joel S; Kwak, June M; Schroeder, Julian I; McKay, John K; Assmann, Sarah M

2016-06-01

1169 I. 1170 II. 1170 III. 1172 IV. 1176 V. 1181 VI. 1182 1183 References 1183 SUMMARY: Modern agriculture is facing multiple challenges including the necessity for a substantial increase in production to meet the needs of a burgeoning human population. Water shortage is a deleterious consequence of both population growth and climate change and is one of the most severe factors limiting global crop productivity. Brassica species, particularly canola varieties, are cultivated worldwide for edible oil, animal feed, and biodiesel, and suffer dramatic yield loss upon drought stress. The recent release of the Brassica napus genome supplies essential genetic information to facilitate identification of drought-related genes and provides new information for agricultural improvement in this species. Here we summarize current knowledge regarding drought responses of canola, including physiological and -omics effects of drought. We further discuss knowledge gained through translational biology based on discoveries in the closely related reference species Arabidopsis thaliana and through genetic strategies such as genome-wide association studies and analysis of natural variation. Knowledge of drought tolerance/resistance responses in canola together with research outcomes arising from new technologies and methodologies will inform novel strategies for improvement of drought tolerance and yield in this and other important crop species. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

[Regular pattern of pain reaction by pressing along the Governor Vessel on the back in patients with digestive system disease].

PubMed

Yang, Guang-Yin; Xu, Jin-Sen; Wu, Zu-Xing

2012-02-01

To find out the regular pattern of pain reaction by pressing along the Governor Vessel at the levels of T3 to L4 on the back in patients with digestive system disease, so as to provide references for diagnosis of the disease. Thirty patients diagnosed with digestive system disease by gastroscopy and colonoscopy as well as 16 patients with digestive symptoms without accurate diagnosis by endoscopy checks were observed. Pressing was applied from Zhiyang (GV 9) to Yaoyangguan (GV 3), including non-acupoints along the Governor Vessel. Positive reaction was recognized since pain, soreness or distension sensation appeared in the space between the spinous process. The positive ratios of 30 patients with endoscopy checks and 16 without the check accounted for 100.0%. The tender spots, usually 5 to 9 in number, were mainly focused on the relative acupoints and non-acupoints along the Governor Vessel from T6 to T10. The pain reaction from Lingtai (GV 10) to Zhongshu (GV 7), which are at the level of T6 to T10, along the Governor Vessel is closely related with digestive system disease. And it can be regarded as function criterion of the digestive system and important reference of auxiliary diagnosis.

[Hikikomori (ひきこもり): a culture-bound syndrome in the web 2.0 era].

PubMed

De Michele, Francesco; Caredda, Maria; Delle Chiaie, Roberto; Salviati, Massimo; Biondi, Massimo

2013-01-01

In 1998 the Japanese psychiatrist Tamaki Sait¯o invented the term hikikomori, referring to a condition characterised by a state of social withdrawal and avoidance (education, work, friendships) combined with a persistent isolation and confinement in the own home for at least 6 months, due to various factors. Initially it undoubtedly regarded a disorder related to a specific socio-cultural context, however in the last couple of years some cases of hikikomori behaviour have also been observed in other countries far from Japan, both geographically and culturally. By way of hypothesis this diffusion can probably be attributed to the cultural revolution represented by mass communication in the internet era; in particular, it seems to be closely related to the immediateness and diffusion of web 2.0, i.e. of social networks. Therefore, we report a case of hikikomori behaviour, who was referred to our ward. M. is a 28-year-old man, who has lived the last 10 years in a state of almost complete isolation. He has maintained contacts with the outside world almost exclusively via internet. He started several therapies with psychiatrists and psychologists, without achieving significant results. The case of M. represents, to our knowledge, the first case of hikikomori described in Italy.

Traces of medical activity in Ephesus.

PubMed

Angeletti, L R

1989-01-01

Ephesus was an important city of Asia Minor, existing as an exchange point between Egypt and the Greek-Roman world. As it was the birthplace of famous physicians and situated between Kos-Knidos and Pergamon, it is surprising that no medical buildings have been clearly identified in this area. In the upper old Hellenistic city, two pillars include, on the southern face, a youth with a goat and Hermes, respectively. On the internal faces, reliefs of tripods, an omphalos, a mortar and a twined snake may refer to mantic and/or pharmacy and medicine. Near the pillars, a temple for sacrifices dedicated to Hera and a statue of Apollo manteion in the Prytaneoion have been found. Because both the Apollo and Hermes myths are closely related to medicine, the pillars may be a sign of medical activity in that part of the city. This activity may be related to both mantic in the direction of the temple and practice in the direction of a building which has not yet been identified. This interpretation is confirmed by an inscription on the Museion-Church of Virgin Mary: a physician from the Mouseion is referred to as a practitioner near the supreme priest (hieros): thus, the pillars may be an indication of both sacred and medical activities in that part of the city.

Cross-linguistic regularities and learner biases reflect “core” mechanics

PubMed Central

Strickland, Brent; Chemla, Emmanuel

2018-01-01

Recent research in infant cognition and adult vision suggests that the mechanical object relationships may be more salient and naturally attention grabbing than similar but non-mechanical relationships. Here we examine two novel sources of evidence from language related to this hypothesis. In Experiments 1 and 2, we show that adults preferentially infer that the meaning of a novel preposition refers to a mechanical as opposed to a non-mechanical relationship. Experiments 3 and 4 examine cross-linguistic adpositions obtained on a large scale from machines or from experts, respectively. While these methods differ in the ease of data collection relative to the reliability of the data, their results converge: we find that across a range of diverse and historically unrelated languages, adpositions (such as prepositions) referring to the mechanical relationships of containment (e.g “in”) and support (e.g. “on”) are systematically shorter than closely matched but not mechanical words such as “behind,” “beside,” “above,” “over,” “out,” and “off.” These results first suggest that languages regularly contain traces of core knowledge representations and that cross-linguistic regularities can therefore be a useful and easily accessible form of information that bears on the foundations of non-linguistic thought. PMID:29324761

Capillary reference half-cell

DOEpatents

Hall, Stephen H.

1996-01-01

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods.

Capillary reference half-cell

DOEpatents

Hall, S.H.

1996-02-13

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods. 11 figs.

Genetics Home Reference: breast cancer

MedlinePlus

... Email Facebook Twitter Home Health Conditions Breast cancer Breast cancer Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Breast cancer is a disease in which certain cells in ...

Closing Regulatory Loopholes Act of 2013

THOMAS, 113th Congress

Sen. Johanns, Mike [R-NE

2013-02-13

Senate - 02/13/2013 Read twice and referred to the Committee on Homeland Security and Governmental Affairs. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Genetics Home Reference: uncombable hair syndrome

MedlinePlus

... Twitter Home Health Conditions Uncombable hair syndrome Uncombable hair syndrome Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Uncombable hair syndrome is a condition that is characterized by ...

Genetics Home Reference: cartilage-hair hypoplasia

MedlinePlus

... Twitter Home Health Conditions Cartilage-hair hypoplasia Cartilage-hair hypoplasia Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Cartilage-hair hypoplasia is a disorder of bone growth characterized ...

Close the Revolving Door Act of 2014

THOMAS, 113th Congress

Sen. Bennet, Michael F. [D-CO

2014-06-03

Senate - 06/03/2014 Read twice and referred to the Committee on Homeland Security and Governmental Affairs. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Genetics Home Reference: juvenile myoclonic epilepsy

MedlinePlus

... Home Health Conditions Juvenile myoclonic epilepsy Juvenile myoclonic epilepsy Printable PDF Open All Close All Enable Javascript ... view the expand/collapse boxes. Description Juvenile myoclonic epilepsy is a condition characterized by recurrent seizures (epilepsy). ...

Genetics Home Reference: pyridoxine-dependent epilepsy

MedlinePlus

... Home Health Conditions Pyridoxine-dependent epilepsy Pyridoxine-dependent epilepsy Printable PDF Open All Close All Enable Javascript ... view the expand/collapse boxes. Description Pyridoxine-dependent epilepsy is a condition that involves seizures beginning in ...

Endocrine glands

MedlinePlus Videos and Cool Tools

... the pancreas, ovaries and testes. The endocrine and nervous systems work very closely together. The brain continuously sends ... endocrine glands. Because of this intimate relationship, the nervous and endocrine systems are referred to as the neuroendocrine system. The ...

Genetics Home Reference: early infantile epileptic encephalopathy 1

MedlinePlus

... infantile epileptic encephalopathy 1 Early infantile epileptic encephalopathy 1 Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Early infantile epileptic encephalopathy 1 (EIEE1) is ...

Genetics Home Reference: congenital bile acid synthesis defect type 1

MedlinePlus

... type 1 Congenital bile acid synthesis defect type 1 Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Congenital bile acid synthesis defect type 1 ...

Genetics Home Reference: Bartter syndrome

MedlinePlus

... Email Facebook Twitter Home Health Conditions Bartter syndrome Bartter syndrome Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Bartter syndrome is a group of very similar kidney disorders ...

Closing Regulatory Loopholes Act of 2011

THOMAS, 112th Congress

Sen. Johanns, Mike [R-NE

2011-09-08

Senate - 09/08/2011 Read twice and referred to the Committee on Homeland Security and Governmental Affairs. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Genetics Home Reference: Parkinson disease

MedlinePlus

... Email Facebook Twitter Home Health Conditions Parkinson disease Parkinson disease Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Parkinson disease is a progressive disorder of the nervous system. ...

Genetics Home Reference: keratoconus

MedlinePlus

... Health Conditions Genes Chromosomes & mtDNA Resources Help Me Understand Genetics Share: Email Facebook Twitter Home Health Conditions Keratoconus Keratoconus Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Keratoconus ...

Genetics Home Reference: sporadic hemiplegic migraine

MedlinePlus

... Home Health Conditions Sporadic hemiplegic migraine Sporadic hemiplegic migraine Printable PDF Open All Close All Enable Javascript ... view the expand/collapse boxes. Description Sporadic hemiplegic migraine is a rare form of migraine headache. Migraines ...

Genetics Home Reference: familial hemiplegic migraine

MedlinePlus

... Home Health Conditions Familial hemiplegic migraine Familial hemiplegic migraine Printable PDF Open All Close All Enable Javascript ... view the expand/collapse boxes. Description Familial hemiplegic migraine is a form of migraine headache that runs ...

Genetics Home Reference: chronic myeloid leukemia

MedlinePlus

... Home Health Conditions Chronic myeloid leukemia Chronic myeloid leukemia Printable PDF Open All Close All Enable Javascript ... view the expand/collapse boxes. Description Chronic myeloid leukemia is a slow-growing cancer of the blood- ...

Genetics Home Reference: arrhythmogenic right ventricular cardiomyopathy

MedlinePlus

... Email Facebook Twitter Home Health Conditions ARVC Arrhythmogenic right ventricular cardiomyopathy Printable PDF Open All Close All ... to view the expand/collapse boxes. Description Arrhythmogenic right ventricular cardiomyopathy ( ARVC ) is a form of heart ...

Genetics Home Reference: isolated Duane retraction syndrome

MedlinePlus

... Health Conditions Isolated Duane retraction syndrome Isolated Duane retraction syndrome Printable PDF Open All Close All Enable ... view the expand/collapse boxes. Description Isolated Duane retraction syndrome is a disorder of eye movement. This ...

Close the Revolving Door Act of 2010

THOMAS, 111th Congress

Sen. Bennet, Michael F. [D-CO

2010-04-28

Senate - 04/28/2010 Read twice and referred to the Committee on Homeland Security and Governmental Affairs. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Genetics Home Reference: Leydig cell hypoplasia

MedlinePlus

... Twitter Home Health Conditions Leydig cell hypoplasia Leydig cell hypoplasia Printable PDF Open All Close All Enable ... consumer genetic testing? What are genome editing and CRISPR-Cas9? What is precision medicine? What is newborn ...

Genetics Home Reference: Cole disease

MedlinePlus

... Email Facebook Twitter Home Health Conditions Cole disease Cole disease Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Cole disease is a disorder that affects the skin. People ...

Genetics Home Reference: cold-induced sweating syndrome

MedlinePlus

... Health Conditions Cold-induced sweating syndrome Cold-induced sweating syndrome Printable PDF Open All Close All Enable ... view the expand/collapse boxes. Description Cold-induced sweating syndrome is characterized by problems with regulating body ...

Genetics Home Reference: congenital hepatic fibrosis

MedlinePlus

... Home Health Conditions Congenital hepatic fibrosis Congenital hepatic fibrosis Printable PDF Open All Close All Enable Javascript ... view the expand/collapse boxes. Description Congenital hepatic fibrosis is a disease of the liver that is ...

Genetics Home Reference: systemic lupus erythematosus

MedlinePlus

... Twitter Home Health Conditions Systemic lupus erythematosus Systemic lupus erythematosus Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Systemic lupus erythematosus (SLE) is a chronic disease that causes ...

46 CFR 56.20-9 - Valve construction.

Code of Federal Regulations, 2010 CFR

2010-10-01

... closed. (b) Valves of Class I piping systems (for restrictions in other classes refer to sections on low temperature service), having diameters exceeding 2 inches must have bolted, pressure seal, or breech lock...

Genetics Home Reference: white sponge nevus

MedlinePlus

... Twitter Home Health Conditions White sponge nevus White sponge nevus Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description White sponge nevus is a condition characterized by the formation ...

Genetics Home Reference: Turner syndrome

MedlinePlus

... Email Facebook Twitter Home Health Conditions Turner syndrome Turner syndrome Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Turner syndrome is a chromosomal condition that affects development in ...

Genetics Home Reference: sialic acid storage disease

MedlinePlus

... Health Conditions Sialic acid storage disease Sialic acid storage disease Printable PDF Open All Close All Enable ... view the expand/collapse boxes. Description Sialic acid storage disease is an inherited disorder that primarily affects ...

Genetics Home Reference: potassium-aggravated myotonia

MedlinePlus

... Facebook Twitter Home Health Conditions Potassium-aggravated myotonia Potassium-aggravated myotonia Printable PDF Open All Close All ... Javascript to view the expand/collapse boxes. Description Potassium-aggravated myotonia is a disorder that affects muscles ...

Genetics Home Reference: pulmonary arterial hypertension

MedlinePlus

... Home Health Conditions Pulmonary arterial hypertension Pulmonary arterial hypertension Printable PDF Open All Close All Enable Javascript ... view the expand/collapse boxes. Description Pulmonary arterial hypertension is a progressive disorder characterized by abnormally high ...

Genetics Home Reference: central core disease

MedlinePlus

... Twitter Home Health Conditions Central core disease Central core disease Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Central core disease is a disorder that affects muscles used ...

Genetics Home Reference: Peters plus syndrome

MedlinePlus

... Facebook Twitter Home Health Conditions Peters plus syndrome Peters plus syndrome Printable PDF Open All Close All ... Javascript to view the expand/collapse boxes. Description Peters plus syndrome is an inherited condition that is ...

Genetics Home Reference: Dubin-Johnson syndrome

MedlinePlus

... Twitter Home Health Conditions Dubin-Johnson syndrome Dubin-Johnson syndrome Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Dubin-Johnson syndrome is a condition characterized by jaundice, which ...

Genetics Home Reference: brain-lung-thyroid syndrome

MedlinePlus

... Twitter Home Health Conditions Brain-lung-thyroid syndrome Brain-lung-thyroid syndrome Printable PDF Open All Close ... Javascript to view the expand/collapse boxes. Description Brain-lung-thyroid syndrome is a group of conditions ...

Genetics Home Reference: Smith-Lemli-Opitz syndrome

MedlinePlus

... Twitter Home Health Conditions Smith-Lemli-Opitz syndrome Smith-Lemli-Opitz syndrome Printable PDF Open All Close ... Javascript to view the expand/collapse boxes. Description Smith-Lemli-Opitz syndrome is a developmental disorder that ...

Genetics Home Reference: rheumatoid arthritis

MedlinePlus

... Email Facebook Twitter Home Health Conditions Rheumatoid arthritis Rheumatoid arthritis Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Rheumatoid arthritis is a disease that causes chronic abnormal inflammation, ...

Genetics Home Reference: factor V deficiency

MedlinePlus

... Twitter Home Health Conditions Factor V deficiency Factor V deficiency Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Factor V deficiency is a rare bleeding disorder. The signs ...

Genetics Home Reference: protein C deficiency

MedlinePlus

... Twitter Home Health Conditions Protein C deficiency Protein C deficiency Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Protein C deficiency is a disorder that increases the risk ...

Genetics Home Reference: X-linked dilated cardiomyopathy

MedlinePlus

... Twitter Home Health Conditions X-linked dilated cardiomyopathy X-linked dilated cardiomyopathy Printable PDF Open All Close ... Javascript to view the expand/collapse boxes. Description X-linked dilated cardiomyopathy is a form of heart ...

Genetics Home Reference: X-linked thrombocytopenia

MedlinePlus

... Facebook Twitter Home Health Conditions X-linked thrombocytopenia X-linked thrombocytopenia Printable PDF Open All Close All ... Javascript to view the expand/collapse boxes. Description X-linked thrombocytopenia is a bleeding disorder that primarily ...

Genetics Home Reference: X-linked myotubular myopathy

MedlinePlus

... Twitter Home Health Conditions X-linked myotubular myopathy X-linked myotubular myopathy Printable PDF Open All Close ... Javascript to view the expand/collapse boxes. Description X-linked myotubular myopathy is a condition that primarily ...

Genetics Home Reference: triple X syndrome

MedlinePlus

... Twitter Home Health Conditions Triple X syndrome Triple X syndrome Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Triple X syndrome , also called trisomy X or 47,XXX, ...

Genetics Home Reference: factor X deficiency

MedlinePlus

... Twitter Home Health Conditions Factor X deficiency Factor X deficiency Printable PDF Open All Close All Enable ... to view the expand/collapse boxes. Description Factor X deficiency is a rare bleeding disorder that varies ...

Genetics Home Reference: X-linked sideroblastic anemia

MedlinePlus

... Twitter Home Health Conditions X-linked sideroblastic anemia X-linked sideroblastic anemia Printable PDF Open All Close ... Javascript to view the expand/collapse boxes. Description X-linked sideroblastic anemia is an inherited disorder that ...

Genetics Home Reference: Kaufman oculocerebrofacial syndrome

MedlinePlus

... Facebook Twitter Home Health Conditions Kaufman oculocerebrofacial syndrome Kaufman oculocerebrofacial syndrome Printable PDF Open All Close All ... Javascript to view the expand/collapse boxes. Description Kaufman oculocerebrofacial syndrome is a disorder characterized by eye ...

Genetics Home Reference: Russell-Silver syndrome

MedlinePlus

... Facebook Twitter Home Health Conditions Russell-Silver syndrome Russell-Silver syndrome Printable PDF Open All Close All ... Javascript to view the expand/collapse boxes. Description Russell-Silver syndrome is a growth disorder characterized by ...

Validity Study of a Jump Mat Compared to the Reference Standard Force Plate.

PubMed

Rogan, Slavko; Radlinger, Lorenz; Imhasly, Caroline; Kneubuehler, Andrea; Hilfiker, Roger

2015-12-01

In the field of vertical jump diagnostics, force plates (FP) are the reference standard. Recently, despite a lack of evidence, jump mats have been used increasingly. Important factors in favor of jumping mats are their low cost and portability. This validity study compared the Haynl-Elektronik jump mat (HE jump mat) with the reference standard force plate. Ten healthy volunteers participated and each participant completed three series of five drop jumps (DJ). The parameters ground contact time (GCT) and vertical jump height (VJH) from the HE jump mat and the FP were used to evaluate the concurrent validity. The following statistical calculations were performed: Pearson's correlation (r), Bland-Altman plots (standard and for adjusted trend), and regression equations. The Bland-Altman plots suggest that the HE jump mat measures shorter contact times and higher jump heights than the FP. The trend-adjusted Bland-Altman plot shows higher mean differences and wider wing-spreads of confidence limits during longer GCT. During the VJH the mean differences and the wing-spreads of the confidence limits throughout the range present as relatively constant. The following regression equations were created, as close as possible to the true value: GCT = 5.920385 + 1.072293 × [value HE jump mat] and VJH = -1.73777 + 1.011156 × [value HE jump mat]. The HE jump mat can be recommended in relation to the validity of constraints. In this study, only a part of the quality criteria were examined. For the final recommendation it is advised to examine the HE jump mat on the other quality criteria (test-retest reliability, sensitivity change).

DNA microarray-based PCR ribotyping of Clostridium difficile.

PubMed

Schneeberg, Alexander; Ehricht, Ralf; Slickers, Peter; Baier, Vico; Neubauer, Heinrich; Zimmermann, Stefan; Rabold, Denise; Lübke-Becker, Antina; Seyboldt, Christian

2015-02-01

This study presents a DNA microarray-based assay for fast and simple PCR ribotyping of Clostridium difficile strains. Hybridization probes were designed to query the modularly structured intergenic spacer region (ISR), which is also the template for conventional and PCR ribotyping with subsequent capillary gel electrophoresis (seq-PCR) ribotyping. The probes were derived from sequences available in GenBank as well as from theoretical ISR module combinations. A database of reference hybridization patterns was set up from a collection of 142 well-characterized C. difficile isolates representing 48 seq-PCR ribotypes. The reference hybridization patterns calculated by the arithmetic mean were compared using a similarity matrix analysis. The 48 investigated seq-PCR ribotypes revealed 27 array profiles that were clearly distinguishable. The most frequent human-pathogenic ribotypes 001, 014/020, 027, and 078/126 were discriminated by the microarray. C. difficile strains related to 078/126 (033, 045/FLI01, 078, 126, 126/FLI01, 413, 413/FLI01, 598, 620, 652, and 660) and 014/020 (014, 020, and 449) showed similar hybridization patterns, confirming their genetic relatedness, which was previously reported. A panel of 50 C. difficile field isolates was tested by seq-PCR ribotyping and the DNA microarray-based assay in parallel. Taking into account that the current version of the microarray does not discriminate some closely related seq-PCR ribotypes, all isolates were typed correctly. Moreover, seq-PCR ribotypes without reference profiles available in the database (ribotype 009 and 5 new types) were correctly recognized as new ribotypes, confirming the performance and expansion potential of the microarray. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Reassortment and evolution of current human influenza A and B viruses.

PubMed

Xu, Xiyan; Lindstrom, Stephen E; Shaw, Michael W; Smith, Catherine B; Hall, Henrietta E; Mungall, Bruce A; Subbarao, Kanta; Cox, Nancy J; Klimov, Alexander

2004-07-01

During the 2001-2002 influenza season, human influenza A (H1N2) reassortant viruses were detected globally. The hemagglutinin (HA) of these H1N2 viruses was similar to that of the A/New Caledonia/20/99 (H1N1) vaccine strain both antigenically and genetically, while their neuraminidase (NA) was antigenically and genetically related to that of recent human influenza H3N2 reference viruses such as A/Moscow/10/99. All six internal genes of the H1N2 reassortants originated from an H3N2 virus. After being detected only in eastern Asia during the past 10 years, Influenza B/Victoria/2/87 lineage viruses reappeared in many countries outside of Asia in 2001. Additionally, reassortant influenza B viruses possessing an HA similar to that of B/Shandong/7/97, a recent B/Victoria/2/87 lineage reference strain, and an NA closely related to that of B/Sichuan/379/99, a recent B/Yamagata/16/88 lineage reference strain, were isolated globally and became the predominant influenza B epidemic strain. The current influenza vaccine is expected to provide good protection against H1N2 viruses because it contains A/New Caledonia/20/99 (H1N1) and A/Panama/2007/99 (H3N2) like viruses whose H1 HA or N2 NA are antigenically similar to those of recent circulating H1N2 viruses. On the other hand, widespread circulation of influenza B Victoria lineage viruses required inclusion of a strain from this lineage in influenza vaccines for the 2002-2003 season.

Classification of HCV and HIV-1 Sequences with the Branching Index

PubMed Central

Hraber, Peter; Kuiken, Carla; Waugh, Mark; Geer, Shaun; Bruno, William J.; Leitner, Thomas

2009-01-01

SUMMARY Classification of viral sequences should be fast, objective, accurate, and reproducible. Most methods that classify sequences use either pairwise distances or phylogenetic relations, but cannot discern when a sequence is unclassifiable. The branching index (BI) combines distance and phylogeny methods to compute a ratio that quantifies how closely a query sequence clusters with a subtype clade. In the hypothesis-testing framework of statistical inference, the BI is compared with a threshold to test whether sufficient evidence exists for the query sequence to be classified among known sequences. If above the threshold, the null hypothesis of no support for the subtype relation is rejected and the sequence is taken as belonging to the subtype clade with which it clusters on the tree. This study evaluates statistical properties of the branching index for subtype classification in HCV and HIV-1. Pairs of BI values with known positive and negative test results were computed from 10,000 random fragments of reference alignments. Sampled fragments were of sufficient length to contain phylogenetic signal that groups reference sequences together properly into subtype clades. For HCV, a threshold BI of 0.71 yields 95.1% agreement with reference subtypes, with equal false positive and false negative rates. For HIV-1, a threshold of 0.66 yields 93.5% agreement. Higher thresholds can be used where lower false positive rates are required. In synthetic recombinants, regions without breakpoints are recognized accurately; regions with breakpoints do not uniquely represent any known subtype. Web-based services for viral subtype classification with the branching index are available online. PMID:18753218

Reference independent species level profiling of the largest marine microbial ecosystem.

NASA Astrophysics Data System (ADS)

Mende, D. R.; DeLong, E.; Aylward, F.

2016-02-01

Marine microbes are of immense importance for the flux of matter and energy within the global oceans. Yet, the temporal variability of microbial communities in response to seasonal and environmental changes remains understudied. In addition, there is only a very limited understanding of the effects that changes within microbial communities at a certain depth have on the other microbes within the water column. Further, existing studies have mostly been limited by the lack of good reference databases. Here we present an reference independent analysis of a year long time series at 5 different water depth of the microbial communities at Station ALOHA, a sampling location representative of the largest contiguous ecosystem on earth, the North Pacific Subtropical Gyre (NPSG). Due to the lack the lack of closely related reference genomes most recent meta-genomic studies of marine microbial ecosystems have been limited to a coarse grained view at higher taxonomic levels. In order to gain a fine grained picture of the microbial communities and their dynamics within the NPSG, we extended the mOTU approach that has been successfully applied to the human microbiome to this marine ecosystem using more than 60 deeply sequenced metagenomic samples. This method allows for species level community profiling and diversity estimates, revealing seasonal shifts within the microbial communities. Additionally, we detected a number of microbes that respond to changes of different changing environmental conditions. We further surveyed the depth specificity of microbes at station ALOHA, showing species specific patterns of presence at different depths.

A Scalable Distributed Syntactic, Semantic, and Lexical Language Model

DTIC Science & Technology

2012-09-01

Here pa(τ) denotes the set of parent states of τ. If the recursive factorization refers to a graph , then we have a Bayesian network (Lauritzen 1996...Broadly speaking, however, the recursive factorization can refer to a representation more complicated than a graph with a fixed set of nodes and edges...factored language (FL) model (Bilmes and Kirchhoff 2003) is close to the smoothing technique we propose here, the major difference is that FL

[Effects of forest gap size and within-gap position on the microclimate in Pinus koraiensis-dominated broadleaved mixed forest].

PubMed

Feng, Jing; Duan, Wen-Biao; Chen, Li-Xin

2012-07-01

HOBO automatic weather stations were installed in the central parts and at the south, north, east, and west edges of large, medium, and small gaps in a Pinus koraiensis-dominated broadleaved mixed forest in Xiaoxing' anling Mountains to measure the air temperature, relative humidity, and photosynthetic photon flux density (PPFD) in these locations and the total radiation and precipitation in the gap centres from June to September 2010, taking the closed forest stand and open field as the controls. The differences in the microclimate between various size forest gaps and between the gap centers and their edges as well as the variations of the microclimatic factors over time were analyzed, and the effects of sunny and overcast days on the diurnal variations of the microclimatic factors within forest gaps were compared, aimed to offer basic data and practice reference for gap regeneration and sustainable management of Pinus koraiensis-dominated broadleaved mixed forest. The PPFD was decreased in the order of large gap, medium gap, and small gap. For the same gaps, the PPFD in gap centre was greater than that in gap edge. The mean monthly air temperature and total radiation in gap centres were declined in the sequence of July, June, August, and September, and the amplitudes of the two climatic factors were decreased in the order of open field, large gap, medium gap, small gap, and closed forest stand. The mean monthly relative humidity in gap centres dropped in the order of August, July, September, and June, and the amplitude of this climatic factor was decreased in the sequence of closed forest stand, small gap, medium gap, large gap, and open field. The total and monthly precipitations for the three different size gaps and open field during measurement period generally decreased in the order of open field, large gap, medium gap, small gap, and closed forest stand. In sunny days, the variations of PPFD, air temperature, and relative humidity were greater in large gap than in small gap, but in overcast days, it was in opposite.

Accurate determination of brain metabolite concentrations using ERETIC as external reference.

PubMed

Zoelch, Niklaus; Hock, Andreas; Heinzer-Schweizer, Susanne; Avdievitch, Nikolai; Henning, Anke

2017-08-01

Magnetic Resonance Spectroscopy (MRS) can provide in vivo metabolite concentrations in standard concentration units if a reliable reference signal is available. For 1 H MRS in the human brain, typically the signal from the tissue water is used as the (internal) reference signal. However, a concentration determination based on the tissue water signal most often requires a reliable estimate of the water concentration present in the investigated tissue. Especially in clinically interesting cases, this estimation might be difficult. To avoid assumptions about the water in the investigated tissue, the Electric REference To access In vivo Concentrations (ERETIC) method has been proposed. In this approach, the metabolite signal is compared with a reference signal acquired in a phantom and potential coil-loading differences are corrected using a synthetic reference signal. The aim of this study, conducted with a transceiver quadrature head coil, was to increase the accuracy of the ERETIC method by correcting the influence of spatial B 1 inhomogeneities and to simplify the quantification with ERETIC by incorporating an automatic phase correction for the ERETIC signal. Transmit field ( B1+) differences are minimized with a volume-selective power optimization, whereas reception sensitivity changes are corrected using contrast-minimized images of the brain and by adapting the voxel location in the phantom measurement closely to the position measured in vivo. By applying the proposed B 1 correction scheme, the mean metabolite concentrations determined with ERETIC in 21 healthy subjects at three different positions agree with concentrations derived with the tissue water signal as reference. In addition, brain water concentrations determined with ERETIC were in agreement with estimations derived using tissue segmentation and literature values for relative water densities. Based on the results, the ERETIC method presented here is a valid tool to derive in vivo metabolite concentration, with potential advantages compared with internal water referencing in diseased tissue. Copyright © 2017 John Wiley & Sons, Ltd.

EEG alpha frequency correlates of burnout and depression: The role of gender.

PubMed

Tement, Sara; Pahor, Anja; Jaušovec, Norbert

2016-02-01

EEG alpha frequency band biomarkers of depression are widely explored. Due to their trait-like features, they may help distinguish between depressive and burnout symptomatology, which is often referred to as "work-related depression". The present correlational study strived to examine whether individual alpha frequency (IAF), power, and coherence in the alpha band can provide evidence for establishing burnout as a separate diagnostic entity. Resting EEG (eyes closed) was recorded in 117 individuals (42 males). In addition, the participants filled-out questionnaires of burnout and depression. Regression analyses highlighted the differential value of IAF and power in predicting burnout and depression. IAF was significantly related to depressive symptomatology, whereas power was linked mostly to burnout. Moreover, seven out of twelve interactions between EEG indicators and gender were significant. Connectivity patterns were significant for depression displaying gender-related differences. The results offer tentative support for establishing burnout as a separate clinical syndrome. Copyright © 2015 Elsevier B.V. All rights reserved.

On the link between column density distribution and density scaling relation in star formation regions

NASA Astrophysics Data System (ADS)

Veltchev, Todor; Donkov, Sava; Stanchev, Orlin

2017-07-01

We present a method to derive the density scaling relation ∝ L^{-α} in regions of star formation or in their turbulent vicinities from straightforward binning of the column-density distribution (N-pdf). The outcome of the method is studied for three types of N-pdf: power law (7/5≤α≤5/3), lognormal (0.7≲α≲1.4) and combination of lognormals. In the last case, the method of Stanchev et al. (2015) was also applied for comparison and a very weak (or close to zero) correlation was found. We conclude that the considered `binning approach' reflects rather the local morphology of the N-pdf with no reference to the physical conditions in a considered region. The rough consistency of the derived slopes with the widely adopted Larson's (1981) value α˜1.1 is suggested to support claims that the density-size relation in molecular clouds is indeed an artifact of the observed N-pdf.

Efficient determination of average valence of manganese in manganese oxides by reaction headspace gas chromatography.

PubMed

Xie, Wei-Qi; Gong, Yi-Xian; Yu, Kong-Xian

2017-08-18

This work investigates a new reaction headspace gas chromatographic (HS-GC) technique for efficient quantifying average valence of manganese (Mn) in manganese oxides. This method is on the basis of the oxidation reaction between manganese oxides and sodium oxalate under the acidic condition. The carbon dioxide (CO 2 ) formed from the oxidation reaction can be quantitatively analyzed by headspace gas chromatography. The data showed that the reaction in the closed headspace vial can be completed in 20min at 80°C. The relative standard deviation of this reaction HS-GC method in the precision testing was within 1.08%, the relative differences between the new method and the reference method (titration method) were no more than 5.71%. The new HS-GC method is automated, efficient, and can be a reliable tool for the quantitative analysis of average valence of manganese in the manganese oxide related research and applications. Copyright © 2017 Elsevier B.V. All rights reserved.

Unified Bayesian Estimator of EEG Reference at Infinity: rREST (Regularized Reference Electrode Standardization Technique)

PubMed Central

Hu, Shiang; Yao, Dezhong; Valdes-Sosa, Pedro A.

2018-01-01

The choice of reference for the electroencephalogram (EEG) is a long-lasting unsolved issue resulting in inconsistent usages and endless debates. Currently, both the average reference (AR) and the reference electrode standardization technique (REST) are two primary, apparently irreconcilable contenders. We propose a theoretical framework to resolve this reference issue by formulating both (a) estimation of potentials at infinity, and (b) determination of the reference, as a unified Bayesian linear inverse problem, which can be solved by maximum a posterior estimation. We find that AR and REST are very particular cases of this unified framework: AR results from biophysically non-informative prior; while REST utilizes the prior based on the EEG generative model. To allow for simultaneous denoising and reference estimation, we develop the regularized versions of AR and REST, named rAR and rREST, respectively. Both depend on a regularization parameter that is the noise to signal variance ratio. Traditional and new estimators are evaluated with this framework, by both simulations and analysis of real resting EEGs. Toward this end, we leverage the MRI and EEG data from 89 subjects which participated in the Cuban Human Brain Mapping Project. Generated artificial EEGs—with a known ground truth, show that relative error in estimating the EEG potentials at infinity is lowest for rREST. It also reveals that realistic volume conductor models improve the performances of REST and rREST. Importantly, for practical applications, it is shown that an average lead field gives the results comparable to the individual lead field. Finally, it is shown that the selection of the regularization parameter with Generalized Cross-Validation (GCV) is close to the “oracle” choice based on the ground truth. When evaluated with the real 89 resting state EEGs, rREST consistently yields the lowest GCV. This study provides a novel perspective to the EEG reference problem by means of a unified inverse solution framework. It may allow additional principled theoretical formulations and numerical evaluation of performance. PMID:29780302

Dietary advice for muscularity, leanness and weight control in Men's Health magazine: a content analysis.

PubMed

Cook, Toni M; Russell, Jean M; Barker, Margo E

2014-10-11

The dietary content of advice in men's lifestyle magazines has not been closely scrutinised. We carried out an analysis of such content in all 2009 issues (n = 11) of Men's Health (MH) focusing on muscularity, leanness and weight control. Promotion of a mesomorphic body image underpinned advice to affect muscle building and control weight. Diet advice was underpinned by a strong pseudo-scientific discourse, with citation of expert sources widely used to legitimise the information. Frequently multiple dietary components were advocated within one article e.g. fat, omega-3 fatty acids, thiamine, zinc and high-glycaemic index foods. Furthermore advice would cover numerous nutritional effects, e.g. strengthening bones, reducing stress and boosting testosterone, with little contextualisation. The emphasis on attainment of a mesomorphic body image permitted promotion of slimming diets.Advice to increase calorie and protein intake to augment muscle mass was frequent (183 and 262 references, respectively). Such an anabolic diet was advised in various ways, including consumption of traditional protein foods (217 references) and sports foods (107 references), thereby replicating muscle magazines' support for nutritional supplements. Although advice to increase consumption of red meat was common (52 references), fish and non-flesh sources of protein (eggs, nuts & pulses, and soy products) together exceeded red meat in number of recommendations (206 references). Advice widely asserted micronutrients and phytochemicals from plant food (161 references) as being important in muscle building. This emphasis diverges from stereotypical gender-based food consumption patterns.Dietary advice for control of body weight largely replicated that of muscularity, with strong endorsement to consume fruits and vegetables (59 references), diets rich in nuts and pulses and fish (66 references), as well as specific micronutrients and phytochemicals (62 references). Notably there was emphasis on fat-burning, good fats and consumption of single foods, with relatively little mention of dietary restriction. Despite the widespread use of scientific information to endorse dietary advice, the content, format and scientific basis of dietary content of MH leaves much to be desired. The dietary advice as provided may not be conducive to public health.

The effect of voluntariness on the acceptance of e-learning by nursing students.

PubMed

Žvanut, Boštjan; Pucer, Patrik; Ličen, Sabina; Trobec, Irena; Plazar, Nadja; Vavpotič, Damjan

2011-05-01

Although e-learning is an innovation that is worth making generally available, it is not always accepted by nursing students. Many researchers state that voluntariness is closely related to the individual level of adoption of innovations. Hence, we hypothesized that voluntariness moderates the effect of perceived attributes of innovations (e.g. relative advantage, compatibility, complexity, trialability, and observability), which determines the acceptance of e-learning. To test the hypothesis a survey involving two groups of nursing students was carried out. For the first group the usage of e-learning was mandatory, for the second group it was optional. The results confirm our hypothesis. Institutions, interested in e-learning initiatives, should consider the effect of voluntariness when implementing e-learning. This paper provides a useful reference that can help e-learning providers to develop guidelines that can improve the acceptance of e-learning. Copyright © 2010 Elsevier Ltd. All rights reserved.

Health care decisions by Sukuma 'peasant intellectuals': a case of radical empiricism?

PubMed

Stroeken, Koen

2012-04-01

Health care decisions in Sukuma-speaking rural communities in Tanzania reproduce a practical epistemology that could be described as radically empiricist, rather than just pluralist; their point of reference is the deeper 'relation' between events, which collective traditions articulate and subjects may experience, but which escapes the atomistic perception privileged by biomedicine. This analysis relies on a diverse portfolio of ethnographic data, including the use and structure of medicinal recipes, the choices of mental health care according to experienced 'effectiveness', and lay discussions on the correct aetiology and treatment of reproductive disorder. Combining two dimensions for a given medical epistemology, the (empirical/ habitual) basis of its transmission and the (open/closed) relation with other epistemologies, four types are proposed: monism, dualism, pluralism, and radical empiricism. The concept of peasant intellectuals, it is argued, needs to be rethought in contexts of medicinal initiation.

A visual identification key utilizing both gestalt and analytic approaches to identification of Carices present in North America (Plantae, Cyperaceae)

PubMed Central

2013-01-01

Abstract Images are a critical part of the identification process because they enable direct, immediate and relatively unmediated comparisons between a specimen being identified and one or more reference specimens. The Carices Interactive Visual Identification Key (CIVIK) is a novel tool for identification of North American Carex species, the largest vascular plant genus in North America, and two less numerous closely-related genera, Cymophyllus and Kobresia. CIVIK incorporates 1288 high-resolution tiled image sets that allow users to zoom in to view minute structures that are crucial at times for identification in these genera. Morphological data are derived from the earlier Carex Interactive Identification Key (CIIK) which in turn used data from the Flora of North America treatments. In this new iteration, images can be viewed in a grid or histogram format, allowing multiple representations of data. In both formats the images are fully zoomable. PMID:24723777

Self-adjoint elliptic operators with boundary conditions on not closed hypersurfaces

NASA Astrophysics Data System (ADS)

Mantile, Andrea; Posilicano, Andrea; Sini, Mourad

2016-07-01

The theory of self-adjoint extensions of symmetric operators is used to construct self-adjoint realizations of a second-order elliptic differential operator on Rn with linear boundary conditions on (a relatively open part of) a compact hypersurface. Our approach allows to obtain Kreĭn-like resolvent formulae where the reference operator coincides with the ;free; operator with domain H2 (Rn); this provides an useful tool for the scattering problem from a hypersurface. Concrete examples of this construction are developed in connection with the standard boundary conditions, Dirichlet, Neumann, Robin, δ and δ‧-type, assigned either on a (n - 1) dimensional compact boundary Γ = ∂ Ω or on a relatively open part Σ ⊂ Γ. Schatten-von Neumann estimates for the difference of the powers of resolvents of the free and the perturbed operators are also proven; these give existence and completeness of the wave operators of the associated scattering systems.

On civil engineering disasters and their mitigation

NASA Astrophysics Data System (ADS)

Xie, Lili; Qu, Zhe

2018-01-01

Civil engineering works such as buildings and infrastructure are the carriers of human civilization. They are, however, also the origins of various types of disasters, which are referred to in this paper as civil engineering disasters. This paper presents the concept of civil engineering disasters, their characteristics, classification, causes, and mitigation technologies. Civil engineering disasters are caused primarily by civil engineering defects, which are usually attributed to improper selection of construction site, hazard assessment, design and construction, occupancy, and maintenance. From this viewpoint, many so-called natural disasters such as earthquakes, strong winds, floods, landslides, and debris flows are substantially due to civil engineering defects rather than the actual natural hazards. Civil engineering disasters occur frequently and globally and are the most closely related to human beings among all disasters. This paper emphasizes that such disasters can be mitigated mainly through civil engineering measures, and outlines the related objectives and scientific and technological challenges.

The pragmatics of "madness": performance analysis of a Bangladeshi woman's "aberrant" lament.

PubMed

Wilce, J M

1998-03-01

A fine-grained analysis of the transcript of a Bangladeshi woman's lament is used to argue for an anthropology of "madness" that attends closely to performance and performativity. The emergent, interactive production of wept speech, together with the conflicting use to which it is put by the performer and her relatives, is linked problematically to performance genres and to ethnopsychiatric indexes of madness. Tuneful weeping is taken by relatives to be performative of madness, in a sense something like Austin's. Yet, exploration of the divergent linguistic ideologies which are brought to bear on the lament not only enables more nuanced ethnographic treatment but also has reflexive ramifications for medical and psychological anthropology. This leads to a critique of the referentialism in our own treatment of language. The role played by transparent reference is overshadowed by indexicality and by dialogical processes of proposing and resisting labels for speech genres attributed to the "mad."

Lurking systematics in dust-based estimates of galaxy ISM masses

NASA Astrophysics Data System (ADS)

Janowiecki, Steven; Cortese, Luca; Catinella, Barbara; Goodwin, Adelle

2018-01-01

We use galaxies from the Herschel Reference Survey to evaluate commonly used indirect predictors of cold gas masses. With observations of cold neutral atomic and molecular gas, we calibrate predictive relationships using infrared dust emission and gas depletion time methods. We derive a set of self-consistent predictions of cold gas masses with ~20% scatter, and the greatest accuracy for total cold gas mass. However, significant systematic residuals are found in all calibrations which depend strongly on the molecular-to-atomic hydrogen mass ratio, and they can over/under-predict gas masses by >0.5 dex. Extending these types of indirect predictions to high-z galaxies (e.g., using ALMA observations of dust continuum to determine gas masses) requires implicit assumptions about the conditions in their interstellar medium. Any scaling relations derived using predicted gas masses may be more closely related to the calibrations used than to the actual galaxies observed.

[Beginnings of the study of Western sciences, "Rangaku," in Kyoto and Osaka in close relations with the Edo school].

PubMed

Murata, T

2001-01-01

Beginnings of the study of Western sciences, "Rangaku," in Kyoto and Osaka in the late 18th century are reviewed. Profiles and achievements of several active learners are briefly described; they include Koishi Genzui (Osaka-Kyoto), Tsuji Ranshitsu (Kyoto), Kimura Kenkadō (Osaka), and Hashimoto Sōkichi (Osaka). Genzui, after having successfully made good relations with Sugita Genpaku and Ohtsuki Gentaku, leading masters of the Edo school, played a role of an advocator for promoting "Rangaku" in Osaka and Kyoto. As a result, Kenkadō attained the publication of his book "Ikkaku Sankō," a monograph of the Western crude drug unicorn, with the help of Gentaku, who had translated a Dutch reference into Japanese for him. Ranshitsu and Sōkichi were taught the Dutch language by Gentaku; Sōkichi is known as the founder of "Rangaku" in Osaka.

"I didn't know her, but…": parasocial mourning of mediated deaths on Facebook RIP pages

NASA Astrophysics Data System (ADS)

Klastrup, Lisbeth

2015-04-01

This article examines the use of six Danish "Rest in Peace" or (RIP) memorial pages. The article focuses on the relation between news media and RIP page use, in relation to general communicative practices on these pages. Based on an analysis of press coverage of the deaths of six young people and a close analysis of 1,015 comments extracted from the RIP pages created to memorialize them, it is shown that their deaths attracted considerable media attention, as did the RIP pages themselves. Comment activity seem to reflect the news stories in the way the commenters refer to the context of death and the emotional distress they experience, but mainly comments on the RIP pages are conventional expressions of sympathy and "RIP" wishes. The article concludes that public RIP pages might be understood as virtual spontaneous shrines, affording an emerging practice of "RIP-ing."

Rapid hybrid de novo assembly of a microbial genome using only short reads: Corynebacterium pseudotuberculosis I19 as a case study.

PubMed

Cerdeira, Louise Teixeira; Carneiro, Adriana Ribeiro; Ramos, Rommel Thiago Jucá; de Almeida, Sintia Silva; D'Afonseca, Vivian; Schneider, Maria Paula Cruz; Baumbach, Jan; Tauch, Andreas; McCulloch, John Anthony; Azevedo, Vasco Ariston Carvalho; Silva, Artur

2011-08-01

Due to the advent of the so-called Next-Generation Sequencing (NGS) technologies the amount of monetary and temporal resources for whole-genome sequencing has been reduced by several orders of magnitude. Sequence reads can be assembled either by anchoring them directly onto an available reference genome (classical reference assembly), or can be concatenated by overlap (de novo assembly). The latter strategy is preferable because it tends to maintain the architecture of the genome sequence the however, depending on the NGS platform used, the shortness of read lengths cause tremendous problems the in the subsequent genome assembly phase, impeding closing of the entire genome sequence. To address the problem, we developed a multi-pronged hybrid de novo strategy combining De Bruijn graph and Overlap-Layout-Consensus methods, which was used to assemble from short reads the entire genome of Corynebacterium pseudotuberculosis strain I19, a bacterium with immense importance in veterinary medicine that causes Caseous Lymphadenitis in ruminants, principally ovines and caprines. Briefly, contigs were assembled de novo from the short reads and were only oriented using a reference genome by anchoring. Remaining gaps were closed using iterative anchoring of short reads by craning to gap flanks. Finally, we compare the genome sequence assembled using our hybrid strategy to a classical reference assembly using the same data as input and show that with the availability of a reference genome, it pays off to use the hybrid de novo strategy, rather than a classical reference assembly, because more genome sequences are preserved using the former. Copyright © 2011 Elsevier B.V. All rights reserved.

Closed-Loop Estimation of Retinal Network Sensitivity by Local Empirical Linearization

PubMed Central

2018-01-01

Abstract Understanding how sensory systems process information depends crucially on identifying which features of the stimulus drive the response of sensory neurons, and which ones leave their response invariant. This task is made difficult by the many nonlinearities that shape sensory processing. Here, we present a novel perturbative approach to understand information processing by sensory neurons, where we linearize their collective response locally in stimulus space. We added small perturbations to reference stimuli and tested if they triggered visible changes in the responses, adapting their amplitude according to the previous responses with closed-loop experiments. We developed a local linear model that accurately predicts the sensitivity of the neural responses to these perturbations. Applying this approach to the rat retina, we estimated the optimal performance of a neural decoder and showed that the nonlinear sensitivity of the retina is consistent with an efficient encoding of stimulus information. Our approach can be used to characterize experimentally the sensitivity of neural systems to external stimuli locally, quantify experimentally the capacity of neural networks to encode sensory information, and relate their activity to behavior. PMID:29379871

Emotion-induced blindness reflects competition at early and late processing stages: an ERP study.

PubMed

Kennedy, Briana L; Rawding, Jennifer; Most, Steven B; Hoffman, James E

2014-12-01

Emotion-induced blindness (EIB) refers to impaired awareness of items appearing soon after an irrelevant, emotionally arousing stimulus. Superficially, EIB appears to be similar to the attentional blink (AB), a failure to report a target that closely follows another relevant target. Previous studies of AB using event-related potentials suggest that the AB results from interference with selection (N2 component) and consolidation (P3b component) of the second target into working memory. The present study applied a similar analysis to EIB and, similarly, found that an irrelevant emotional distractor suppressed the N2 and P3b components associated with the following target at short lags. Emotional distractors also elicited a positive deflection that appeared to be similar to the PD component, which has been associated with attempts to suppress salient, irrelevant distractors (Kiss, Grubert, Petersen, & Eimer, 2012; Sawaki, Geng, & Luck, 2012; Sawaki & Luck, 2010). These results suggest that irrelevant emotional pictures gain access to working memory, even when observers are attempting to ignore them and, like the AB, prevent access of a closely following target.

Genetics Home Reference: supravalvular aortic stenosis

MedlinePlus

... Twitter Home Health Conditions Supravalvular aortic stenosis Supravalvular aortic stenosis Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Supravalvular aortic stenosis (SVAS) is a heart defect that develops before ...

Genetics Home Reference: spinocerebellar ataxia type 6

MedlinePlus

... Twitter Home Health Conditions SCA6 Spinocerebellar ataxia type 6 Printable PDF Open All Close All Enable Javascript ... the expand/collapse boxes. Description Spinocerebellar ataxia type 6 ( SCA6 ) is a condition characterized by progressive problems ...

Genetics Home Reference: inherited thyroxine-binding globulin deficiency

MedlinePlus

... Health Conditions Inherited thyroxine-binding globulin deficiency Inherited thyroxine-binding globulin deficiency Printable PDF Open All Close ... to view the expand/collapse boxes. Description Inherited thyroxine-binding globulin deficiency is a genetic condition that ...

Genetics Home Reference: thiamine-responsive megaloblastic anemia syndrome

MedlinePlus

... Thiamine-responsive megaloblastic anemia syndrome Thiamine-responsive megaloblastic anemia syndrome Printable PDF Open All Close All Enable ... the expand/collapse boxes. Description Thiamine-responsive megaloblastic anemia syndrome is a rare condition characterized by hearing ...

Genetics Home Reference: familial male-limited precocious puberty

MedlinePlus

... male-limited precocious puberty Familial male-limited precocious puberty Printable PDF Open All Close All Enable Javascript ... expand/collapse boxes. Description Familial male-limited precocious puberty is a condition that causes early sexual development ...

Genetics Home Reference: PDGFRB-associated chronic eosinophilic leukemia

MedlinePlus

... associated chronic eosinophilic leukemia PDGFRB-associated chronic eosinophilic leukemia Printable PDF Open All Close All Enable Javascript ... expand/collapse boxes. Description PDGFRB -associated chronic eosinophilic leukemia is a type of cancer of blood-forming ...

Genetics Home Reference: factor VII deficiency

MedlinePlus

... Facebook Twitter Home Health Conditions Factor VII deficiency Factor VII deficiency Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Factor VII deficiency is a rare bleeding disorder that varies ...

49 CFR 1012.7 - Meetings which may be closed to the public.

Code of Federal Regulations, 2010 CFR

2010-10-01

... withholding or refers to particular types of matters to be withheld. (4) Disclose trade secrets or commercial... criminal investigation, or by an agency conducting a lawful national security intelligence investigation...

Genetics Home Reference: glycogen storage disease type V

MedlinePlus

... Health Conditions Glycogen storage disease type V Glycogen storage disease type V Printable PDF Open All Close ... to view the expand/collapse boxes. Description Glycogen storage disease type V (also known as GSDV or ...

Genetics Home Reference: familial encephalopathy with neuroserpin inclusion bodies

MedlinePlus

... Home Health Conditions FENIB Familial encephalopathy with neuroserpin inclusion bodies Printable PDF Open All Close All Enable ... expand/collapse boxes. Description Familial encephalopathy with neuroserpin inclusion bodies ( FENIB ) is a disorder that causes progressive ...

Genetics Home Reference: glycogen storage disease type IX

MedlinePlus

... Health Conditions Glycogen storage disease type IX Glycogen storage disease type IX Printable PDF Open All Close ... to view the expand/collapse boxes. Description Glycogen storage disease type IX (also known as GSD IX) ...

Analytic studies of the hard dumbell fluid

NASA Astrophysics Data System (ADS)

Morriss, G. P.; Cummings, P. T.

A closed form analytic theory for the structure of the hard dumbell fluid is introduced and evaluated. It is found to be comparable in accuracy to the reference interaction site approximation (RISA) of Chandler and Andersen.

Genetics Home Reference: hereditary myopathy with early respiratory failure

MedlinePlus

... Home Health Conditions HMERF Hereditary myopathy with early respiratory failure Printable PDF Open All Close All Enable ... expand/collapse boxes. Description Hereditary myopathy with early respiratory failure ( HMERF ) is an inherited muscle disease that ...

Genetics Home Reference: distal hereditary motor neuropathy, type II

MedlinePlus

... hereditary motor neuropathy, type II Distal hereditary motor neuropathy, type II Printable PDF Open All Close All ... the expand/collapse boxes. Description Distal hereditary motor neuropathy, type II is a progressive disorder that affects ...

Ellipsoidal terrain correction based on multi-cylindrical equal-area map projection of the reference ellipsoid

NASA Astrophysics Data System (ADS)

Ardalan, A. A.; Safari, A.

2004-09-01

An operational algorithm for computation of terrain correction (or local gravity field modeling) based on application of closed-form solution of the Newton integral in terms of Cartesian coordinates in multi-cylindrical equal-area map projection of the reference ellipsoid is presented. Multi-cylindrical equal-area map projection of the reference ellipsoid has been derived and is described in detail for the first time. Ellipsoidal mass elements with various sizes on the surface of the reference ellipsoid are selected and the gravitational potential and vector of gravitational intensity (i.e. gravitational acceleration) of the mass elements are computed via numerical solution of the Newton integral in terms of geodetic coordinates {λ,ϕ,h}. Four base- edge points of the ellipsoidal mass elements are transformed into a multi-cylindrical equal-area map projection surface to build Cartesian mass elements by associating the height of the corresponding ellipsoidal mass elements to the transformed area elements. Using the closed-form solution of the Newton integral in terms of Cartesian coordinates, the gravitational potential and vector of gravitational intensity of the transformed Cartesian mass elements are computed and compared with those of the numerical solution of the Newton integral for the ellipsoidal mass elements in terms of geodetic coordinates. Numerical tests indicate that the difference between the two computations, i.e. numerical solution of the Newton integral for ellipsoidal mass elements in terms of geodetic coordinates and closed-form solution of the Newton integral in terms of Cartesian coordinates, in a multi-cylindrical equal-area map projection, is less than 1.6×10-8 m2/s2 for a mass element with a cross section area of 10×10 m and a height of 10,000 m. For a mass element with a cross section area of 1×1 km and a height of 10,000 m the difference is less than 1.5×10-4m2/s2. Since 1.5× 10-4 m2/s2 is equivalent to 1.5×10-5m in the vertical direction, it can be concluded that a method for terrain correction (or local gravity field modeling) based on closed-form solution of the Newton integral in terms of Cartesian coordinates of a multi-cylindrical equal-area map projection of the reference ellipsoid has been developed which has the accuracy of terrain correction (or local gravity field modeling) based on the Newton integral in terms of ellipsoidal coordinates.

An Application of Close-Up Photogrammetry in Viticulture

NASA Astrophysics Data System (ADS)

Grazia D'Urso, Maria; Marino, Costantino Luis

2016-06-01

Within the context of Geomatic-based Agriculture an application of close-up photogrammetry is illustrated with reference to precision farming. The application has been carried out in a particularly attractive archaeological site and concerns some viticulture species produced in situ by following a farming technique dated about two thousand years. Specifically we illustrate and comment the photogrammetric close-range acquisitions, carried out both outdoor and indoor, aimed at producing a digital botanic atlas that can adequately replace the traditional ones drawn by hand. High precision geometric measurements of vineyard leaves and grapes, are suitable indices of their growth rate and an objective estimate of the relevant repening degree.

3-D Imagery Cockpit Display Development

DTIC Science & Technology

1990-08-01

and ownship appeared in the lower right corner of the CLF, in the form XXX NM. The third data line showed individual aircraft altitude in the form XX K...data line showed aircraft heading in the form XXX ° . 4.2.5.3 Close-Look Format Options The Close-Look Format option switches are shown in Figure 4.2...vector, it is Need for declutter levels, redundant. Need range readout. Needs basic altitude reference. Low altitude warning system with sexy female

HAI, a new airborne, absolute, twin dual-channel, multi-phase TDLAS-hygrometer: background, design, setup, and first flight data

NASA Astrophysics Data System (ADS)

Buchholz, Bernhard; Afchine, Armin; Klein, Alexander; Schiller, Cornelius; Krämer, Martina; Ebert, Volker

2017-01-01

The novel Hygrometer for Atmospheric Investigation (HAI) realizes a unique concept for simultaneous gas-phase and total (gas-phase + evaporated cloud particles) water measurements. It has been developed and successfully deployed for the first time on the German HALO research aircraft. This new instrument combines direct tunable diode laser absorption spectroscopy (dTDLAS) with a first-principle evaluation method to allow absolute water vapor measurements without any initial or repetitive sensor calibration using a reference gas or a reference humidity generator. HAI contains two completely independent dual-channel (closed-path, open-path) spectrometers, one at 1.4 and one at 2.6 µm, which together allow us to cover the entire atmospheric H2O range from 1 to 40 000 ppmv with a single instrument. Both spectrometers each comprise a separate, wavelength-individual extractive, closed-path cell for total water (ice and gas-phase) measurements. Additionally, both spectrometers couple light into a common open-path cell outside of the aircraft fuselage for a direct, sampling-free, and contactless determination of the gas-phase water content. This novel twin dual-channel setup allows for the first time multiple self-validation functions, in particular a reliable, direct, in-flight validation of the open-path channels. During the first field campaigns, the in-flight deviations between the independent and calibration-free channels (i.e., closed-path to closed-path and open-path to closed-path) were on average in the 2 % range. Further, the fully autonomous HAI hygrometer allows measurements up to 240 Hz with a minimal integration time of 1.4 ms. The best precision is achieved by the 1.4 µm closed-path cell at 3.8 Hz (0.18 ppmv) and by the 2.6 µm closed-path cell at 13 Hz (0.055 ppmv). The requirements, design, operation principle, and first in-flight performance of the hygrometer are described and discussed in this work.

Surgical margins in head and neck squamous cell carcinoma: what is 'close'?

PubMed

Alicandri-Ciufelli, Matteo; Bonali, Marco; Piccinini, Alessia; Marra, Laura; Ghidini, Angelo; Cunsolo, Elio Maria; Maiorana, Antonino; Presutti, Livio; Conte, Pier Franco

2013-09-01

The aim of this systematic review is to evaluate the definition of close margin in head and neck squamous cell carcinoma (HNSCC), and its possible prognostic significance. An appropriate string was run on PubMed to retrieve articles discussing the 'close' surgical margin issue in HNSCC. A double cross-check was performed on citations and full-text articles retrieved. In total, 348 articles were identified. Further references were included by using the option "Titles in your search terms" option in PubMed. 15 papers were finally included for qualitative synthesis. In vocal cord surgery of HNSCC, a close margin could be considered to be ≤1 mm, in the larynx ≤5 mm, in the oral cavity ≤4 mm, and in the oropharynx ≤5 mm. In each patient, the choice of extent of close margin should be balanced against general condition, tumor stage, and functional issues to indicate appropriate adjuvant therapy.

Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data.

PubMed

Nishito, Yukari; Osana, Yasunori; Hachiya, Tsuyoshi; Popendorf, Kris; Toyoda, Atsushi; Fujiyama, Asao; Itaya, Mitsuhiro; Sakakibara, Yasubumi

2010-04-16

Bacillus subtilis natto is closely related to the laboratory standard strain B. subtilis Marburg 168, and functions as a starter for the production of the traditional Japanese food "natto" made from soybeans. Although re-sequencing whole genomes of several laboratory domesticated B. subtilis 168 derivatives has already been attempted using short read sequencing data, the assembly of the whole genome sequence of a closely related strain, B. subtilis natto, from very short read data is more challenging, particularly with our aim to assemble one fully connected scaffold from short reads around 35 bp in length. We applied a comparative genome assembly method, which combines de novo assembly and reference guided assembly, to one of the B. subtilis natto strains. We successfully assembled 28 scaffolds and managed to avoid substantial fragmentation. Completion of the assembly through long PCR experiments resulted in one connected scaffold for B. subtilis natto. Based on the assembled genome sequence, our orthologous gene analysis between natto BEST195 and Marburg 168 revealed that 82.4% of 4375 predicted genes in BEST195 are one-to-one orthologous to genes in 168, with two genes in-paralog, 3.2% are deleted in 168, 14.3% are inserted in BEST195, and 5.9% of genes present in 168 are deleted in BEST195. The natto genome contains the same alleles in the promoter region of degQ and the coding region of swrAA as the wild strain, RO-FF-1. These are specific for gamma-PGA production ability, which is related to natto production. Further, the B. subtilis natto strain completely lacked a polyketide synthesis operon, disrupted the plipastatin production operon, and possesses previously unidentified transposases. The determination of the whole genome sequence of Bacillus subtilis natto provided detailed analyses of a set of genes related to natto production, demonstrating the number and locations of insertion sequences that B. subtilis natto harbors but B. subtilis 168 lacks. Multiple genome-level comparisons among five closely related Bacillus species were also carried out. The determined genome sequence of B. subtilis natto and gene annotations are available from the Natto genome browser http://natto-genome.org/.

Radar Observation of Large Attenuation in Convective Storms: Implications for the Dropsize Distribution

NASA Technical Reports Server (NTRS)

Tian, Lin; Heymsfield, G. M.; Srivastava, R. C.

2000-01-01

Airborne meteorological radars typically operate at attenuating wavelengths. The path integrated attenuation (PIA) can be estimated using the surface reference technique (SRT). In this method, an initial value is determined for the radar cross section of the earth surface in a rain-free area in relatively close proximity to the rain cloud. During subsequent observations of precipitation any decrease 'in the observed surface cross section from the reference value s assumed to be a result of the two-way attenuation along the propagation path. In this paper we present selected instances of high PIA observed over land by an airborne radar. The observations were taken in Brazil and Florida during TRMM (Tropical Rainfall Measurement Mission) field campaigns. We compared these observations with collocated and nearly simultaneous ground-based radar observations by an S-band radar that is not subject to significant attenuation. In this preliminary evaluation, a systematic difference in the attenuation in the two storms is attributed to a difference in the raindrop size distributions; this is supported by observations of ZDR (differential reflectivity).

Perturbation theory of structure in classical liquid mixtures: Application to metallic systems near phase separation. Ph.D. Thesis

NASA Technical Reports Server (NTRS)

Henderson, R. L.

1974-01-01

The partial structure factors of classical simple liquid mixtures near phase separation are dicussed. The theory is developed for particles interacting through pair potentials, and is thus appropriate both to insulating fluids, and also to metallic systems if these may be described by an effective ion-ion pair interaction. The motivation arose from consideration of metallic liquid mixtures, in which resistive anomalies have been observed near phase separation. A mean field theory correction appropriate to 3 pair potential for the effects of correlated motions in the reference fluid is studied. The work is cast in terms of functions which are closely related to the direct correlation functions of Ornstein and Zernike. The results are qualitatively in accord with physical expectations. Quantitative agreement with experiment seems to turn on the selection of the hard core reference potential in terms of the metallic effective pair potential. It is suggested that the present effective pair potentials are perhaps not properly used to calculate the metallic structure factors at long wavelength.

[On the notes of the Xinkan Huangdimingtangjiujing (see text for symbol) in the possession of the National Diet Library].

PubMed

Amano, Yosuke

2014-12-01

This paper analyzes the notes of Xinkan huangdimingtangjiujing which are in the possession of the National Diet Library, particularly referring to those notes which include "master said", or "master's theory". Some of those are found in the answers from Manase Dosan (see text for symbol) to Hata Soha (see text for symbol) collected in the Kotei meido kyukyo hushin shosho (see text for symbol) which is the record of the question and answer letters between Manase Dosan and Hata Soha. This research indicates that this volume has a close relation to the acupuncture research of Dosan's or Soha's school. And it indicates that the "master" in the Notes of Xinkan huangdimingtangjiujing refers to Dosan. Acupuncture research has been flourishing since the Edo era and has compiled a lot of volumes on acupuncture. This volume contains some pioneering pieces of this research done in the Azuchi Momoyama era by Dosan and Soha, and is a precious material, showing that some of the state of affairs of the research done in that age still remains today.

Unlocking the genetic diversity of Creole wheats.

PubMed

Vikram, Prashant; Franco, Jorge; Burgueño-Ferreira, Juan; Li, Huihui; Sehgal, Deepmala; Saint Pierre, Carolina; Ortiz, Cynthia; Sneller, Clay; Tattaris, Maria; Guzman, Carlos; Sansaloni, Carolina Paola; Ellis, Mark; Fuentes-Davila, Guillermo; Reynolds, Matthew; Sonders, Kai; Singh, Pawan; Payne, Thomas; Wenzl, Peter; Sharma, Achla; Bains, Navtej Singh; Singh, Gyanendra Pratap; Crossa, José; Singh, Sukhwinder

2016-03-15

Climate change and slow yield gains pose a major threat to global wheat production. Underutilized genetic resources including landraces and wild relatives are key elements for developing high-yielding and climate-resilient wheat varieties. Landraces introduced into Mexico from Europe, also known as Creole wheats, are adapted to a wide range of climatic regimes and represent a unique genetic resource. Eight thousand four hundred and sixteen wheat landraces representing all dimensions of Mexico were characterized through genotyping-by-sequencing technology. Results revealed sub-groups adapted to specific environments of Mexico. Broadly, accessions from north and south of Mexico showed considerable genetic differentiation. However, a large percentage of landrace accessions were genetically very close, although belonged to different regions most likely due to the recent (nearly five centuries before) introduction of wheat in Mexico. Some of the groups adapted to extreme environments and accumulated high number of rare alleles. Core reference sets were assembled simultaneously using multiple variables, capturing 89% of the rare alleles present in the complete set. Genetic information about Mexican wheat landraces and core reference set can be effectively utilized in next generation wheat varietal improvement.

Electrothermal atomic absorption spectrometric determination of copper in nickel-base alloys with various chemical modifiers*1

NASA Astrophysics Data System (ADS)

Tsai, Suh-Jen Jane; Shiue, Chia-Chann; Chang, Shiow-Ing

1997-07-01

The analytical characteristics of copper in nickel-base alloys have been investigated with electrothermal atomic absorption spectrometry. Deuterium background correction was employed. The effects of various chemical modifiers on the analysis of copper were investigated. Organic modifiers which included 2-(5-bromo-2-pyridylazo)-5-(diethylamino-phenol) (Br-PADAP), ammonium citrate, 1-(2-pyridylazo)-naphthol, 4-(2-pyridylazo)resorcinol, ethylenediaminetetraacetic acid and Triton X-100 were studied. Inorganic modifiers palladium nitrate, magnesium nitrate, aluminum chloride, ammonium dihydrogen phosphate, hydrogen peroxide and potassium nitrate were also applied in this work. In addition, zirconium hydroxide and ammonium hydroxide precipitation methods have also been studied. Interference effects were effectively reduced with Br-PADAP modifier. Aqueous standards were used to construct the calibration curves. The detection limit was 1.9 pg. Standard reference materials of nickel-base alloys were used to evaluate the accuracy of the proposed method. The copper contents determined with the proposed method agreed closely with the certified values of the reference materials. The recoveries were within the range 90-100% with relative standard deviation of less than 10%. Good precision was obtained.

PaPrBaG: A machine learning approach for the detection of novel pathogens from NGS data

NASA Astrophysics Data System (ADS)

Deneke, Carlus; Rentzsch, Robert; Renard, Bernhard Y.

2017-01-01

The reliable detection of novel bacterial pathogens from next-generation sequencing data is a key challenge for microbial diagnostics. Current computational tools usually rely on sequence similarity and often fail to detect novel species when closely related genomes are unavailable or missing from the reference database. Here we present the machine learning based approach PaPrBaG (Pathogenicity Prediction for Bacterial Genomes). PaPrBaG overcomes genetic divergence by training on a wide range of species with known pathogenicity phenotype. To that end we compiled a comprehensive list of pathogenic and non-pathogenic bacteria with human host, using various genome metadata in conjunction with a rule-based protocol. A detailed comparative study reveals that PaPrBaG has several advantages over sequence similarity approaches. Most importantly, it always provides a prediction whereas other approaches discard a large number of sequencing reads with low similarity to currently known reference genomes. Furthermore, PaPrBaG remains reliable even at very low genomic coverages. CombiningPaPrBaG with existing approaches further improves prediction results.

Serious play: A comparative study of engagement and learning in hobby practices

NASA Astrophysics Data System (ADS)

Azevedo, Flavio Saraiva

This dissertation is concerned with human behavior usually attributed to individual interests. As conceptualized in experimental and educational psychology, an individual interest refers to a particular relationship (called an interest relationship) between a person and a set of topically related activities. Interest relationships are self-motivated, long-term, and relatively stable, though they might change over time. As an example, a teenager's enduring and self-motivated involvement with model rocketry reflects her individual interest in the topic or domain of rocketry. As an alternative to this formulation, I develop a practice-centered theory of persistent engagement---or, simply, a theory of persistence---in free-choice, self-motivated practices. Taken together, self-motivated, long-term persistence in a free-choice practice refers exactly to the same set of phenomena that is the purview of psychological theories of individual interests. Hobbies are prototypic of free-choice, self-motivated, extended pursuit practices and therefore are the focal object of study of this dissertation. The centerpiece of the theory I put forward is the grounded theoretical concept of lines of practice. A line of practice is a distinctive, recurrent pattern of engagement in a person's continued pursuit of a hobby. To return to the example above, our rocketry buff might have three lines of practice in her hobby---one related to designing and flying unusual rockets, the other related to designing and flying high-powered rockets, and yet a third line concerned with designing and flying low-powered models. Any particular line of practice entails a set of closely interrelated activities. For instance, designing and flying high-powered rockets might entail visiting particular hobby stores, becoming a member of particular rocketry clubs, and engaging in construction work. Designing and flying unusual rockets, on the other hand, might entail some very different activities (e.g., continuously searching for unusual materials that can be transformed into rockets), as well as activities that cross line boundaries (e.g., construction work). The "essence" that runs through a set of closely related activities is what defines a line of practice. Such an "essence" is determined by two structural elements. The first element is the preferences that a hobbyist brings to bear on her practice. Preferences refer to psychological constructs such as goals, beliefs, values, and so forth. Our hypothetical subject's line of unusual rockets might reflect her need to fashion an identity as a competent practitioner and her love of design and construction. The second structural element refers to the conditions of practice impinging on the persons hobby. Conditions of practice include such things as the material infrastructure available to the practitioner (e.g., tools for working rocket parts), the immediate circumstances of the hobbyist's life (e.g., the person's socio-economic status and whether or not the person has easy access to practice sites), the rules of practice adopted by a hobby community (e.g., the maximum ceiling for rocket flights), the larger socio-cultural space in which the hobby exists (e.g., the availability of hobby stores), and so on. Each of these factors, and the many sub-factors included therein, may be seen to provide constraints and affordances for practice. I illustrate the theory with a comparative study of two hobby practices with techno-scientific underpinnings---model rocketry and amateur astronomy. The empirical base of the work is a series of ethnographic video records of two focal subjects, one for each hobby practice. (Abstract shortened by UMI.)

Quantifying navigational information: The catchment volumes of panoramic snapshots in outdoor scenes.

PubMed

Murray, Trevor; Zeil, Jochen

2017-01-01

Panoramic views of natural environments provide visually navigating animals with two kinds of information: they define locations because image differences increase smoothly with distance from a reference location and they provide compass information, because image differences increase smoothly with rotation away from a reference orientation. The range over which a given reference image can provide navigational guidance (its 'catchment area') has to date been quantified from the perspective of walking animals by determining how image differences develop across the ground plane of natural habitats. However, to understand the information available to flying animals there is a need to characterize the 'catchment volumes' within which panoramic snapshots can provide navigational guidance. We used recently developed camera-based methods for constructing 3D models of natural environments and rendered panoramic views at defined locations within these models with the aim of mapping navigational information in three dimensions. We find that in relatively open woodland habitats, catchment volumes are surprisingly large extending for metres depending on the sensitivity of the viewer to image differences. The size and the shape of catchment volumes depend on the distance of visual features in the environment. Catchment volumes are smaller for reference images close to the ground and become larger for reference images at some distance from the ground and in more open environments. Interestingly, catchment volumes become smaller when only above horizon views are used and also when views include a 1 km distant panorama. We discuss the current limitations of mapping navigational information in natural environments and the relevance of our findings for our understanding of visual navigation in animals and autonomous robots.

Quantifying navigational information: The catchment volumes of panoramic snapshots in outdoor scenes

PubMed Central

Zeil, Jochen

2017-01-01

Panoramic views of natural environments provide visually navigating animals with two kinds of information: they define locations because image differences increase smoothly with distance from a reference location and they provide compass information, because image differences increase smoothly with rotation away from a reference orientation. The range over which a given reference image can provide navigational guidance (its ‘catchment area’) has to date been quantified from the perspective of walking animals by determining how image differences develop across the ground plane of natural habitats. However, to understand the information available to flying animals there is a need to characterize the ‘catchment volumes’ within which panoramic snapshots can provide navigational guidance. We used recently developed camera-based methods for constructing 3D models of natural environments and rendered panoramic views at defined locations within these models with the aim of mapping navigational information in three dimensions. We find that in relatively open woodland habitats, catchment volumes are surprisingly large extending for metres depending on the sensitivity of the viewer to image differences. The size and the shape of catchment volumes depend on the distance of visual features in the environment. Catchment volumes are smaller for reference images close to the ground and become larger for reference images at some distance from the ground and in more open environments. Interestingly, catchment volumes become smaller when only above horizon views are used and also when views include a 1 km distant panorama. We discuss the current limitations of mapping navigational information in natural environments and the relevance of our findings for our understanding of visual navigation in animals and autonomous robots. PMID:29088300

Phylogeny of the ammonia-producing ruminal bacteria Peptostreptococcus anaerobius, Clostridium sticklandii, and Clostridium aminophilum sp. nov

NASA Technical Reports Server (NTRS)

Paster, B. J.; Russell, J. B.; Yang, C. M.; Chow, J. M.; Woese, C. R.; Tanner, R.

1993-01-01

In previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. Three isolates, strains C, FT (T = type strain), and SR, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. On the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. In this study, the 16S rRNA sequences of these isolates and related bacteria were determined to establish the phylogenetic positions of the organisms. The sequences of strains C, FT, and SR and reference strains of Peptostreptococcus anaerobius, Clostridium sticklandii, Clostridium coccoides, Clostridium aminovalericum, Acetomaculum ruminis, Clostridium leptum, Clostridium lituseburense, Clostridium acidiurici, and Clostridium barkeri were determined by using a modified Sanger dideoxy chain termination method. Strain C, a large coccus purported to belong to the genus Peptostreptococcus, was closely related to P. anaerobius, with a level of sequence similarity of 99.6%. Strain SR, a heat-resistant, short, rod-shaped organism, was closely related to C. sticklandii, with a level of sequence similarity of 99.9%. However, strain FT, a heat-resistant, pleomorphic, rod-shaped organism, was only distantly related to some clostridial species and P. anaerobius. On the basis of the sequence data, it was clear that strain FT warranted designation as a separate species. The closest known relative of strain FT was C. coccoides (level of similarity, only 90.6%). Additional strains that are phenotypically similar to strain FT were isolated in this study.(ABSTRACT TRUNCATED AT 250 WORDS).

Reference values for developing responsive functional outcome measures across the lifespan.

PubMed

McKay, Marnee J; Baldwin, Jennifer N; Ferreira, Paulo; Simic, Milena; Vanicek, Natalie; Burns, Joshua

2017-04-18

To generate a reference dataset of commonly performed functional outcome measures in 1,000 children and adults and investigate the influence of demographic, anthropometric, strength, and flexibility characteristics. Twelve functional outcome measures were collected from 1,000 healthy individuals aged 3-101 years: 6-minute walk test, 30-second chair stand test, timed stairs test, long jump, vertical jump, choice stepping reaction time, balance (Star Excursion Balance Test, tandem stance eyes open and closed, single-leg stance eyes closed), and dexterity (9-hole peg test, Functional Dexterity Test). Correlation and multiple regression analyses were performed to identify factors independently associated with each measure. Age- and sex-stratified reference values for functional outcome measures were generated. Functional performance increased through childhood and adolescence, plateaued during adulthood, and declined in older adulthood. While balance did not differ between the sexes, male participants generally performed better at gross motor tasks while female participants performed better at dexterous tasks. Height was the most consistent correlate of functional performance in children, while lower limb muscle strength was a major determinant in adolescents and adults. In older adults, age, lower limb strength, and joint flexibility explained up to 63% of the variance in functional measures. These normative reference values provide a framework to accurately track functional decline associated with neuromuscular disorders and assist development and validation of responsive outcome measures for therapeutic trials. © 2017 American Academy of Neurology.

Flexible add-on solution for MR image-guided interventions in a closed-bore scanner environment.

PubMed

Busse, Harald; Garnov, Nikita; Thörmer, Gregor; Zajonz, Dirk; Gründer, Wilfried; Kahn, Thomas; Moche, Michael

2010-09-01

MRI is of great clinical utility for the guidance of various diagnostic and therapeutic procedures. In a standard closed-bore scanner, the simplest approach is to manipulate the instrument outside the bore and move the patient into the bore for reference and control imaging only. Without navigational assistance, however, such an approach can be difficult, inaccurate, and time consuming. Therefore, an add-on navigation solution is described that addresses these limitations. Patient registration is established by an automatic, robust, and fast (<30 sec) localization of table-mounted MR reference markers and the instrument is tracked optically. Good hand-eye coordination is provided by following the virtual instrument on MR images that are reconstructed in real time from the reference data. Needle displacements of 2.2 +/- 0.6 mm and 3.9 +/- 2.4 mm were determined in a phantom (P < 0.05), depending on whether the reference markers were placed at smaller (98-139 mm) or larger (147-188 mm) distances from the isocenter. Clinical functionality of the navigation concept is demonstrated by a double oblique, subscapular hook-wire insertion in a patient with a body mass index of 30.1 kg/m(2). Ease of use, compactness, and flexibility of this technique suggest that it can be used for many other procedures in different body regions. More patient cases are needed to evaluate clinical performance and workflow. 2010 Wiley-Liss, Inc.

Robust model reference adaptive output feedback tracking for uncertain linear systems with actuator fault based on reinforced dead-zone modification.

PubMed

Bagherpoor, H M; Salmasi, Farzad R

2015-07-01

In this paper, robust model reference adaptive tracking controllers are considered for Single-Input Single-Output (SISO) and Multi-Input Multi-Output (MIMO) linear systems containing modeling uncertainties, unknown additive disturbances and actuator fault. Two new lemmas are proposed for both SISO and MIMO, under which dead-zone modification rule is improved such that the tracking error for any reference signal tends to zero in such systems. In the conventional approach, adaption of the controller parameters is ceased inside the dead-zone region which results tracking error, while preserving the system stability. In the proposed scheme, control signal is reinforced with an additive term based on tracking error inside the dead-zone which results in full reference tracking. In addition, no Fault Detection and Diagnosis (FDD) unit is needed in the proposed approach. Closed loop system stability and zero tracking error are proved by considering a suitable Lyapunov functions candidate. It is shown that the proposed control approach can assure that all the signals of the close loop system are bounded in faulty conditions. Finally, validity and performance of the new schemes have been illustrated through numerical simulations of SISO and MIMO systems in the presence of actuator faults, modeling uncertainty and output disturbance. Copyright © 2015 ISA. Published by Elsevier Ltd. All rights reserved.

Nature of Medical Malpractice Claims Against Radiation Oncologists.

PubMed

Marshall, Deborah; Tringale, Kathryn; Connor, Michael; Punglia, Rinaa; Recht, Abram; Hattangadi-Gluth, Jona

2017-05-01

To examine characteristics of medical malpractice claims involving radiation oncologists closed during a 10-year period. Malpractice claims filed against radiation oncologists from 2003 to 2012 collected by a nationwide liability insurance trade association were analyzed. Outcomes included the nature of claims and indemnity payments, including associated presenting diagnoses, procedures, alleged medical errors, and injury severity. We compared the likelihood of a claim resulting in payment in relation to injury severity categories (death as referent) using binomial logistic regression. There were 362 closed claims involving radiation oncology, 102 (28%) of which were paid, resulting in $38 million in indemnity payments. The most common alleged errors included "improper performance" (38% of closed claims, 18% were paid; 29% [$11 million] of total indemnity), "errors in diagnosis" (25% of closed claims, 46% were paid; 44% [$17 million] of total indemnity), and "no medical misadventure" (14% of closed claims, 8% were paid; less than 1% [$148,000] of total indemnity). Another physician was named in 32% of claims, and consent issues/breach of contract were cited in 18%. Claims for injury resulting in death represented 39% of closed claims and 25% of total indemnity. "Improper performance" was the primary alleged error associated with injury resulting in death. Compared with claims involving death, major temporary injury (odds ratio [OR] 2.8, 95% confidence interval [CI] 1.29-5.85, P=.009), significant permanent injury (OR 3.1, 95% CI 1.48-6.46, P=.003), and major permanent injury (OR 5.5, 95% CI 1.89-16.15, P=.002) had a higher likelihood of a claim resulting in indemnity payment. Improper performance was the most common alleged malpractice error. Claims involving significant or major injury were more likely to be paid than those involving death. Insights into the nature of liability claims against radiation oncologists may help direct efforts to improve quality of care and minimize the risk of being sued. Copyright © 2017 Elsevier Inc. All rights reserved.

Nature of Medical Malpractice Claims Against Radiation Oncologists

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marshall, Deborah; Tringale, Kathryn; Connor, Michael

Purpose: To examine characteristics of medical malpractice claims involving radiation oncologists closed during a 10-year period. Methods and Materials: Malpractice claims filed against radiation oncologists from 2003 to 2012 collected by a nationwide liability insurance trade association were analyzed. Outcomes included the nature of claims and indemnity payments, including associated presenting diagnoses, procedures, alleged medical errors, and injury severity. We compared the likelihood of a claim resulting in payment in relation to injury severity categories (death as referent) using binomial logistic regression. Results: There were 362 closed claims involving radiation oncology, 102 (28%) of which were paid, resulting in $38more » million in indemnity payments. The most common alleged errors included “improper performance” (38% of closed claims, 18% were paid; 29% [$11 million] of total indemnity), “errors in diagnosis” (25% of closed claims, 46% were paid; 44% [$17 million] of total indemnity), and “no medical misadventure” (14% of closed claims, 8% were paid; less than 1% [$148,000] of total indemnity). Another physician was named in 32% of claims, and consent issues/breach of contract were cited in 18%. Claims for injury resulting in death represented 39% of closed claims and 25% of total indemnity. “Improper performance” was the primary alleged error associated with injury resulting in death. Compared with claims involving death, major temporary injury (odds ratio [OR] 2.8, 95% confidence interval [CI] 1.29-5.85, P=.009), significant permanent injury (OR 3.1, 95% CI 1.48-6.46, P=.003), and major permanent injury (OR 5.5, 95% CI 1.89-16.15, P=.002) had a higher likelihood of a claim resulting in indemnity payment. Conclusions: Improper performance was the most common alleged malpractice error. Claims involving significant or major injury were more likely to be paid than those involving death. Insights into the nature of liability claims against radiation oncologists may help direct efforts to improve quality of care and minimize the risk of being sued.« less

Dynamics of the Transition Corona

NASA Technical Reports Server (NTRS)

Masson, Sophie; McCauley, Patrick; Golub, Leon; Reeves, Katharine K.; DeLuca, Edward E.

2014-01-01

Magnetic reconnection between the open and closed magnetic fields in the corona is believed to play a crucial role in the corona/heliosphere coupling. At large scale, the exchange of open/closed connectivity is expected to occur in pseudo-streamer (PS) structures. However, there is neither clear observational evidence of how such coupling occurs in PSs, nor evidence for how the magnetic reconnection evolves. Using a newly developed technique, we enhance the off-limb magnetic fine structures observed with the Atmospheric Imaging Assembly and identify a PS-like feature located close to the northern coronal hole. We first identify that the magnetic topology associated with the observation is a PS, null-point (NP) related topology bounded by the open field. By comparing the magnetic field configuration with the extreme ultraviolet (EUV) emission regions, we determined that most of the magnetic flux associated with plasma emission are small loops below the PS basic NP and open field bounding the PS topology. In order to interpret the evolution of the PS, we referred to a three-dimensional MHD interchange reconnection modeling the exchange of connectivity between small closed loops and the open field. The observed PS fine structures follow the dynamics of the magnetic field before and after reconnecting at the NP obtained by the interchange model. Moreover, the pattern of the EUV plasma emission is the same as the shape of the expected plasma emission location derived from the simulation. These morphological and dynamical similarities between the PS observations and the results from the simulation strongly suggest that the evolution of the PS, and in particular the opening/closing of the field, occurs via interchange/slipping reconnection at the basic NP of the PS. Besides identifying the mechanism at work in the large-scale coupling between the open and closed fields, our results highlight that interchange reconnection in PSs is a gradual physical process that differs from the impulsive reconnection of the solar-jet model.

Genetics Home Reference: STXBP1 encephalopathy with epilepsy

MedlinePlus

... Conditions STXBP1 encephalopathy with epilepsy STXBP1 encephalopathy with epilepsy Printable PDF Open All Close All Enable Javascript ... the expand/collapse boxes. Description STXBP1 encephalopathy with epilepsy is a condition characterized by recurrent seizures (epilepsy), ...

Genetics Home Reference: spinal muscular atrophy with progressive myoclonic epilepsy

MedlinePlus

... myoclonic epilepsy Spinal muscular atrophy with progressive myoclonic epilepsy Printable PDF Open All Close All Enable Javascript ... boxes. Description Spinal muscular atrophy with progressive myoclonic epilepsy (SMA-PME) is a neurological condition that causes ...

Genetics Home Reference: autosomal dominant partial epilepsy with auditory features

MedlinePlus

... Twitter Home Health Conditions ADPEAF Autosomal dominant partial epilepsy with auditory features Printable PDF Open All Close ... the expand/collapse boxes. Description Autosomal dominant partial epilepsy with auditory features ( ADPEAF ) is an uncommon form ...

Communication with Your Family and Your Doctor about Your Wishes

MedlinePlus

... close to the end of your life. References: http://www.aarp.org/families/end_life/ http://www.nlm.nih.gov/medlineplus/endoflifeissues.html http://agnewsarchive.tamu.edu/dailynews/stories/CFAM/Sep0407a.htm ...

Genetics Home Reference: Senior-Løken syndrome

MedlinePlus

... Facebook Twitter Home Health Conditions Senior-Løken syndrome Senior-Løken syndrome Printable PDF Open All Close All ... Javascript to view the expand/collapse boxes. Description Senior-Løken syndrome is a rare disorder characterized by ...

Genetics Home Reference: 46,XX testicular disorder of sex development

MedlinePlus

... of sex development 46,XX testicular disorder of sex development Printable PDF Open All Close All Enable ... collapse boxes. Description 46,XX testicular disorder of sex development is a condition in which individuals with ...

Genetics Home Reference: neuropathy, ataxia, and retinitis pigmentosa

MedlinePlus

... Twitter Home Health Conditions NARP Neuropathy, ataxia, and retinitis pigmentosa Printable PDF Open All Close All Enable Javascript ... the expand/collapse boxes. Description Neuropathy, ataxia, and retinitis pigmentosa ( NARP ) is a condition that causes a variety ...

Genetics Home Reference: Meckel syndrome

MedlinePlus

... when a structure called the neural tube, a layer of cells that ultimately develops into the brain and spinal cord, fails to close completely during the first few weeks of embryonic development. Meckel syndrome can also cause problems with ...

Genetics Home Reference: hereditary leiomyomatosis and renal cell cancer

MedlinePlus

... Home Health Conditions HLRCC Hereditary leiomyomatosis and renal cell cancer Printable PDF Open All Close All Enable Javascript ... expand/collapse boxes. Description Hereditary leiomyomatosis and renal cell cancer ( HLRCC ) is a disorder in which affected individuals ...

Genetics Home Reference: nephrogenic diabetes insipidus

MedlinePlus

... Nephrogenic diabetes insipidus Nephrogenic diabetes insipidus Printable PDF Open All Close All Enable Javascript to view the expand/collapse boxes. Description Nephrogenic diabetes insipidus is a disorder of water balance. The body normally balances fluid intake with the ...

Genetics Home Reference: immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome

MedlinePlus

... Health Conditions IPEX syndrome Immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome Printable PDF Open All Close All ... expand/collapse boxes. Description Immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome primarily affects males and is ...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hess, E.; Hamilton, D.

The purpose of this ITER is to chronicle the development of the ROST (trademark), its capabilities, associated equipment, and accessories. The report concludes with an evaluation of how closely the results obtained using the technology compare to the results obtained using the reference methods.

Application of adaptive antenna techniques to future commercial satellite communication

NASA Technical Reports Server (NTRS)

Ersoy, L.; Lee, E. A.; Matthews, E. W.

1987-01-01

The purpose of this contract was to identify the application of adaptive antenna technique in future operational commercial satellite communication systems and to quantify potential benefits. The contract consisted of two major subtasks. Task 1, Assessment of Future Commercial Satellite System Requirements, was generally referred to as the Adaptive section. Task 2 dealt with Pointing Error Compensation Study for a Multiple Scanning/Fixed Spot Beam Reflector Antenna System and was referred to as the reconfigurable system. Each of these tasks was further sub-divided into smaller subtasks. It should also be noted that the reconfigurable system is usually defined as an open-loop system while the adaptive system is a closed-loop system. The differences between the open- and closed-loop systems were defined. Both the adaptive and reconfigurable systems were explained and the potential applications of such systems were presented in the context of commercial communication satellite systems.

Application of adaptive antenna techniques to future commercial satellite communications. Executive summary

NASA Technical Reports Server (NTRS)

Ersoy, L.; Lee, E. A.; Matthews, E. W.

1987-01-01

The purpose of this contract was to identify the application of adaptive antenna technique in future operational commercial satellite communication systems and to quantify potential benefits. The contract consisted of two major subtasks. Task 1, Assessment of Future Commercial Satellite System Requirements, was generally referred to as the Adaptive section. Task 2 dealt with Pointing Error Compensation Study for a Multiple Scanning/Fixed Spot Beam Reflector Antenna System and was referred to as the reconfigurable system. Each of these tasks was further subdivided into smaller subtasks. It should also be noted that the reconfigurable system is usually defined as an open-loop system while the adaptive system is a closed-loop system. The differences between the open- and closed-loop systems were defined. Both the adaptive and reconfigurable systems were explained and the potential applications of such systems were presented in the context of commercial communication satellite systems.

Adaptive control and noise suppression by a variable-gain gradient algorithm

NASA Technical Reports Server (NTRS)

Merhav, S. J.; Mehta, R. S.

1987-01-01

An adaptive control system based on normalized LMS filters is investigated. The finite impulse response of the nonparametric controller is adaptively estimated using a given reference model. Specifically, the following issues are addressed: The stability of the closed loop system is analyzed and heuristically established. Next, the adaptation process is studied for piecewise constant plant parameters. It is shown that by introducing a variable-gain in the gradient algorithm, a substantial reduction in the LMS adaptation rate can be achieved. Finally, process noise at the plant output generally causes a biased estimate of the controller. By introducing a noise suppression scheme, this bias can be substantially reduced and the response of the adapted system becomes very close to that of the reference model. Extensive computer simulations validate these and demonstrate assertions that the system can rapidly adapt to random jumps in plant parameters.

A Comparative Study of Precise Point Positioning (PPP) Accuracy Using Online Services

NASA Astrophysics Data System (ADS)

Malinowski, Marcin; Kwiecień, Janusz

2016-12-01

Precise Point Positioning (PPP) is a technique used to determine the position of receiver antenna without communication with the reference station. It may be an alternative solution to differential measurements, where maintaining a connection with a single RTK station or a regional network of reference stations RTN is necessary. This situation is especially common in areas with poorly developed infrastructure of ground stations. A lot of research conducted so far on the use of the PPP technique has been concerned about the development of entire day observation sessions. However, this paper presents the results of a comparative analysis of accuracy of absolute determination of position from observations which last between 1 to 7 hours with the use of four permanent services which execute calculations with PPP technique such as: Automatic Precise Positioning Service (APPS), Canadian Spatial Reference System Precise Point Positioning (CSRS-PPP), GNSS Analysis and Positioning Software (GAPS) and magicPPP - Precise Point Positioning Solution (magicGNSS). On the basis of acquired results of measurements, it can be concluded that at least two-hour long measurements allow acquiring an absolute position with an accuracy of 2-4 cm. An evaluation of the impact on the accuracy of simultaneous positioning of three points test network on the change of the horizontal distance and the relative height difference between measured triangle vertices was also conducted. Distances and relative height differences between points of the triangular test network measured with a laser station Leica TDRA6000 were adopted as references. The analyses of results show that at least two hours long measurement sessions can be used to determine the horizontal distance or the difference in height with an accuracy of 1-2 cm. Rapid products employed in calculations conducted with PPP technique reached the accuracy of determining coordinates on a close level as in elaborations which employ Final products.

Model Reduction via Principe Component Analysis and Markov Chain Monte Carlo (MCMC) Methods

NASA Astrophysics Data System (ADS)

Gong, R.; Chen, J.; Hoversten, M. G.; Luo, J.

2011-12-01

Geophysical and hydrogeological inverse problems often include a large number of unknown parameters, ranging from hundreds to millions, depending on parameterization and problems undertaking. This makes inverse estimation and uncertainty quantification very challenging, especially for those problems in two- or three-dimensional spatial domains. Model reduction technique has the potential of mitigating the curse of dimensionality by reducing total numbers of unknowns while describing the complex subsurface systems adequately. In this study, we explore the use of principal component analysis (PCA) and Markov chain Monte Carlo (MCMC) sampling methods for model reduction through the use of synthetic datasets. We compare the performances of three different but closely related model reduction approaches: (1) PCA methods with geometric sampling (referred to as 'Method 1'), (2) PCA methods with MCMC sampling (referred to as 'Method 2'), and (3) PCA methods with MCMC sampling and inclusion of random effects (referred to as 'Method 3'). We consider a simple convolution model with five unknown parameters as our goal is to understand and visualize the advantages and disadvantages of each method by comparing their inversion results with the corresponding analytical solutions. We generated synthetic data with noise added and invert them under two different situations: (1) the noised data and the covariance matrix for PCA analysis are consistent (referred to as the unbiased case), and (2) the noise data and the covariance matrix are inconsistent (referred to as biased case). In the unbiased case, comparison between the analytical solutions and the inversion results show that all three methods provide good estimates of the true values and Method 1 is computationally more efficient. In terms of uncertainty quantification, Method 1 performs poorly because of relatively small number of samples obtained, Method 2 performs best, and Method 3 overestimates uncertainty due to inclusion of random effects. However, in the biased case, only Method 3 correctly estimates all the unknown parameters, and both Methods 1 and 2 provide wrong values for the biased parameters. The synthetic case study demonstrates that if the covariance matrix for PCA analysis is inconsistent with true models, the PCA methods with geometric or MCMC sampling will provide incorrect estimates.

Peritoneal mucinous cystadenocarcinoma of probable urachal origin: a challenging diagnosis

PubMed Central

Gore, D M; Bloch, S; Waller, W; Cohen, P

2006-01-01

This report describes the case of a mucinous cystadenocarcinoma of probable urachal origin that presented with mass effect, precipitating deep venous thrombosis and pulmonary embolism. The patient presented with acute symptoms of leg swelling, pain and dyspnoea, and a vague awareness of lower abdominal distension. Computer tomography showed a cystic mass closely related to the anterior abdominal wall and the superior aspect of the bladder. A 1500 cm3 cyst adherent to the dome of the urinary bladder was resected on laparotomy. Partial cystectomy was not carried out in the belief that the cyst represented a benign lesion. Subsequent imaging has shown cystic changes in the anterior bladder wall, and the patient has been referred for partial cystectomy. PMID:17021133

Future of the world's automobile industry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kagawa, T.

1989-03-01

The author feels that the Japanese automobile industry is approaching a turning point. But it is not enough to refer only to the changing structure of the Japanese automobile industry when similar structural changes are taking place on a worldwide scale. Therefore he first comments on the current state of the automobile industry of the world, and then reviews the reasons for the gap between automobile production and demand and the historical background of this phenomenon. Then, finally, he discusses the current progress of and future challenges in the structural adjustment of the global automobile industry. He notes the verymore » close relation of the battery industry to the automobile industry, and hopes the former will prosper hand in hand with the latter. 6 tables.« less

Metabolic fingerprinting of Angelica sinensis during growth using UPLC-TOFMS and chemometrics data analysis

PubMed Central

2013-01-01

Background The radix of Angelica sinensis is widely used as a medicinal herbal and metabolomics research of this plant during growth is necessary. Results Principal component analysis of the UPLC-QTOFMS data showed that these 27 samples could be separated into 4 different groups. The chemical markers accounting for these separations were identified from the PCA loadings plot. These markers were further verified by accurate mass tandem mass and retention times of available reference standards. The study has shown that accumulation of secondary metabolites of Angelica sinensis is closely related to the growth periods. Conclusions The UPLC-QTOFMS based metabolomics approach has great potential for analysis of the alterations of secondary metabolites of Angelica sinensis during growth. PMID:23453085

An Introduction to the Problem of the Existence of Classical and Quantum Information

NASA Astrophysics Data System (ADS)

Rocchi, Paolo; Gianfagna, Leonida

2006-01-01

Quantum computing raises novel meditation upon the nature of information, notably a number of theorists set out the critical elements of Shannon's work, which currently emerges as the most popular reference in the quantum territory. The present paper follows this vein and highlights how the prerequisites of the information theory, which should detail the precise hypotheses of this theory, appear rather obscure and the problem of the existence of information is still open. This work puts forward a theoretical scheme that calculates the existence of elementary items. These results clarify basic assumptions in information engineering. Later we bring evidence how information is not an absolute quantity and close with a discussion upon the information relativity.

Chen-Nester-Tung quasi-local energy and Wang-Yau quasi-local mass

NASA Astrophysics Data System (ADS)

Liu, Jian-Liang; Yu, Chengjie

2017-10-01

In this paper, we show that the Chen-Nester-Tung (CNT) quasi-local energy with 4D isometric matching references is closely related to the Wang-Yau (WY) quasi-local energy. As a particular example, we compute the second variation of the CNT quasi-local energy for axially symmetric Kerr-like spacetimes with axially symmetric embeddings at the obvious critical point (0 , 0) and find that it is a saddle critical point in most of the cases. Also, as a byproduct, we generalize a previous result about the coincidence of the CNT quasi-local energy and Brown-York mass for axially symmetric Kerr-like spacetimes by Tam and the first author Liu and Tam (2016) to general spacetimes.

Evaluation of two molecular techniques for rapid detection of the main dermatophytic agents of tinea capitis.

PubMed

Deng, S; Zhou, Z; de Hoog, G S; Wang, X; Abliz, P; Sun, J; Najafzadeh, M J; Pan, W; Lei, W; Zhu, S; Hasimu, H; Zhang, P; Guo, Y; Deng, D; Liao, W

2015-12-01

Tinea capitis is very common in Western China, with the most widespread aetiological agent being Trichophyton violaceum, while Microsporum canis is prevalent in the remainder of China. Conventional diagnostics and internal transcribed spacer (ITS) sequencing analyses have proven relatively limited due to the close phylogenetic relationship of anthropophilic dermatophytes. Therefore, alternative molecular tools with sufficient specificity, reproducibility and sensitivity are necessary. To evaluate two molecular techniques [multiplex ligation-dependent probe amplification (MLPA) and rolling circle amplification (RCA)] for rapid detection of the aetiological agents of tinea capitis, T. violaceum and M. canis. Probes of RCA and MLPA were designed with target sequences in the rDNA ITS gene region. Strains tested consist of 31 T. violaceum, 22 M. canis and 24 reference strains of species that are taxonomically close to the target species. The specificity and reproducibility of RCA and MLPA in detection of T. violaceum and M. canis were both 100% in both species. Sensitivity testing showed that RCA was positive at concentrations down to 1·68 × 10(6) copies of DNA in the TvioRCA probe, and 2·7 × 10(8) copies of DNA in McRCA. MLPA yielded positive results at concentrations of DNA down to 1·68 × 10(1) copies in the TvioMLPA probe and 2·7 × 10(2) in McMLPA. The two techniques were sufficiently specific and sensitive for discriminating the target DNA of T. violaceum and M. canis from that of closely related dermatophytes. RCA and MLPA are advantageous in their reliability and ease of operation compared with standard polymerase chain reaction and conventional methods. © 2015 British Association of Dermatologists.

Connection Between the ICRF and the Dynamical Reference Frame for the Outer Planets

NASA Astrophysics Data System (ADS)

da Silva Neto, D. N.; Assafin, M.; Andrei, A. H.; Vieira Martins, R.

2005-01-01

This work brings an approach intending to improve the connection between the Dynamical Reference Frame and the Extragalactic Reference Frame. For that, close encounters of outer Solar System objects and quasars are used. With this goal, Uranus, Neptune and two quasars were observed at Laborat´orio Nacional de Astrof´ısica (LNA), Brazil. The optical reference frame is the HCRF, as given by the UCAC2 catalogue. The first results show an accuracy of 45 mas - 50 mas in the optical positions. The optical minus radio offsets give the local orientation between the catalogue and radio frame. From this, it is possible to place the optical planet coordinates on the extragalactic frame. A comparison between the new corrected optical coordinates and the respective DE ephemeris to these planets can give the instant orientations of the Dynamical Reference Frame with regard to the ICRS, for this zone of outer Solar System.

Kiloniella antarctica sp. nov., isolated from a polynya of Amundsen Sea in Western Antarctic Sea.

PubMed

Si, Ok-Ja; Yang, Hye-Young; Hwang, Chung Yeon; Kim, So-Jeong; Choi, Sun-Bin; Kim, Jong-Geol; Jung, Man-Young; Kim, Song-Gun; Roh, Seong Woon; Rhee, Sung-Keun

2017-07-01

A taxonomic study was conducted on strain soj2014T, which was isolated from the surface water of a polynya in the Antarctic Sea. Comparative 16S rRNA gene sequence analysis showed that strain soj2014T belongs to the family Kiloniellaceae and is closely related to Kiloniella spongiae MEBiC09566T, 'Kiloniella litopenaei' P1-1T and Kiloniella laminariae LD81T (98.0 %, 97.8 % and 96.2 % 16S rRNA gene sequence similarity, respectively). The DNA-DNA hybridization values between strain soj2014T and closely related strains were below 28.6 %. The G+C content of the genomic DNA of strain soj2014T was 45.5 mol%. The predominant cellular fatty acids were summed feature 8 (composed of C18 : 1ω6c/C18 : 1ω7c, 57.0 %) and summed feature 3 (composed of C16 : 1ω6c/C16 : 1ω7c, 23.5 %). Strain soj2014T was Gram-stain-negative, slightly curved, spiral-shaped, and motile with a single polar flagellum. The strain grew at 0-30 °C (optimum, 25 °C), in 1.5-5.1 % (w/v) NaCl (optimum, 2.1-2.4 %) and at pH 5.5-9.5 (optimum, 7.5-8.0). It also had differential carbohydrate utilization traits and enzyme activities compared with closely related strains. Based on these phylogenetic, phenotypic and chemotaxonomic analyses, strain soj2014T represents a distinct species, separable from the reference strains, and is, therefore, proposed as a novel species, Kiloniella antarctica sp. nov. The type strain is soj2014T (=KCTC 42186T=JCM 30386T).

Preliminary study on the time-related changes of the infrared thermal images of the human body

NASA Astrophysics Data System (ADS)

Li, Ziru; Zhang, Xusheng; Lin, Gang; Chen, Zhigang

2009-08-01

It is of great importance to study the manifestations and the influencing factors of the time-related changes of infrared thermal images (ITI) of human body since the variable body surface temperature distribution seriously affected the application of ITI in medicine. In this paper, manifestations of time-related changes of the ITI of human body from three double-blind randomized trials and their correlation with meteorological factors (e.g. temperature, pressure, humidity, cold front passage and tropical cyclone landing) were studied. The trials were placebo or drug controlled studying the influences of Chinese medicine health food (including Shengsheng capsule with immunity adjustment function, Shengan capsule with sleep improvement function and Shengyi capsule with the function of helping to decrease serum lipid) on the ITI of human body. In the first thirty-six days of the trials images were scanned every six days and image data in the seven observation time spots (including the 0, 6, 12, 18, 24, 30, 36 day of the trial) were used for the time-related study. For every subject the scanned time was fixed in the day within two hours. The ITI features which could reflect the functions of the health foods were studied. The indexes of the features were relative magnitude (temperature difference between the viewing area and the reference area). Results showed that the variation tendencies of the trial group and control group were basically the same in placebo controlled trials and some of the long-term effects of Chinese medicine health food could be reflected significantly in certain time spots in the first thirty-six days. Time-related changes of the ITI of human body were closely related with meteorological factors but there were other influencing factors still need to be studied. As the ITI of human body could reflect the influences of Chinese medicine health foods and are closely related with meteorology, there are bright prospects for the application of ITI in health monitor.

Receiving power through confirmation: the meaning of close relatives for people who have been critically ill.

PubMed

Engström, Asa; Söderberg, Siv

2007-09-01

This paper is a report of a study to elucidate the meaning of close relatives for people who have been critically ill and received care in an intensive care unit. Falling critically ill can bring about a difficult change in life. In previous reports such events are described as frightening experiences, and close relatives are described as an important source of support in this difficult situation. A purposive sample of 10 adults, eight men and two women, narrated how they experienced their close relatives during and after the time they were critically ill. The data were collected in 2004. The interview texts were transcribed and interpreted using a phenomenological hermeneutic approach influenced by the philosophy of Ricoeur. One major theme was identified, experiencing confirmation, with six sub-themes: receiving explanations; a feeling of being understood; a feeling of safety; gaining strength and will-power; having possibilities and realizing their value. Close relatives served as tools for the person who was ill, facilitating better communication and an increased ability to do various things. Simultaneously, feelings of dependence on the close relatives were expressed. There were descriptions of loneliness and fear in the absence of close relatives and, in order to feel safe, the participants wanted their close relatives to stay near them. Close relatives are vital, as they are the ill person's motivation to stay alive and to continue the struggle. Their presence is of great importance for the ill person and must be facilitated by staff.

Clinically referred children and adolescents: fathers, family constellations, and other demographic factors.

PubMed

Phares, V; Lum, J J

1997-06-01

Investigated the likelihood that clinically referred youth have contact with their biological father. Family demographics such as family constellation, race/ethnicity, and socioeconomic status were also explored. Based on 356 consecutive therapy and assessment referrals to an outpatient clinic, slightly less than half (42.4%) of the children and adolescents referred due to psychological problems and more than half (67.8%) of the youth referred due to learning difficulties lived with both their biological mother and biological father. For those who did not live with both of their biological parents, 40.0% and 56.0%, respectively, had regular face-to-face contact with both biological parents. These figures suggest that, although the percentages of intact families are somewhat lower in treatment samples than in the general population or in a sample referred for learning difficulties, close to half of clinically referred youth continue to live with both of their biological parents. Future directions for the inclusion of fathers in clinical research are discussed.

40 CFR 270.310 - What equipment information must I keep at my facility?

Code of Federal Regulations, 2011 CFR

2011-07-01

... mechanical seals). (b) For facilities that cannot install a closed-vent system and control device to comply... Gaseous Emissions” (incorporated by reference as specified in 40 CFR 260.11) or other engineering texts...

Genetics Home Reference: myopathy with deficiency of iron-sulfur cluster assembly enzyme

MedlinePlus

... Myopathy with deficiency of iron-sulfur cluster assembly enzyme Printable PDF Open All Close All Enable Javascript ... Myopathy with deficiency of iron-sulfur cluster assembly enzyme is an inherited disorder that primarily affects muscles ...

Going that extra mile: individuals travel further to maintain face-to-face contact with highly related kin than with less related kin.

PubMed

Pollet, Thomas V; Roberts, Sam G B; Dunbar, Robin I M

2013-01-01

The theory of inclusive fitness has transformed our understanding of cooperation and altruism. However, the proximate psychological underpinnings of altruism are less well understood, and it has been argued that emotional closeness mediates the relationship between genetic relatedness and altruism. In this study, we use a real-life costly behaviour (travel time) to dissociate the effects of genetic relatedness from emotional closeness. Participants travelled further to see more closely related kin, as compared to more distantly related kin. For distantly related kin, the level of emotional closeness mediated this relationship--when emotional closeness was controlled for, there was no effect of genetic relatedness on travel time. However, participants were willing to travel further to visit parents, children and siblings as compared to more distantly related kin, even when emotional closeness was controlled for. This suggests that the mediating effect of emotional closeness on altruism varies with levels of genetic relatedness.

A Marine Aerosol Reference Tank system as a breaking wave analogue

NASA Astrophysics Data System (ADS)

Stokes, M. D.; Deane, G. B.; Prather, K.; Bertram, T. H.; Ruppel, M. J.; Ryder, O. S.; Brady, J. M.; Zhao, D.

2012-12-01

In order to better understand the processes governing the production of marine aerosols a repeatable, controlled method for their generation is required. The Marine Aerosol Reference Tank (MART) has been designed to closely approximate oceanic conditions by producing an evolving bubble plume and surface foam patch. The tank utilizes an intermittently plunging sheet of water and large volume tank reservoir to simulate turbulence, plume and foam formation, and is monitored volumetrically and acoustically to ensure the repeatability of conditions.

Ignition and Combustion Studies of Hazard Division 1.1 and 1.3 Substances

DTIC Science & Technology

2010-07-01

Effect of Time at Temperature on Burning Rate. The burning rate of the HD1.1 explosive PBXN -5 is compared to that of neat cyclotetramethylene...tetranitramine (HMX) in Figure 14. The explosive, PBXN -5, is composed of 95 weight percent HMX and 5 percent Viton A as binder. The HMX burning rate...the closed bomb technique (Reference 18). The PBXN -5 was composed of small agglomerates of HMX coated with the binder (Reference 19). The PBXN -5

Title list of documents made publicly available, June 1-30, 1997

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

This document is a monthly publication describing information received and published by the U.S. Nuclear Regulatory Commission (NRC). This information includes (1) docketed material associated with civilian nuclear power plants and other uses of radioactive materials and (2) nondocketed material received and published by NRC pertinent to its role as a regulatory agency. As used here, {open_quotes}docketed{close_quotes} does not refer to Court dockets; it refers to the system by which NRC maintains its regulatory records.

OWL references in ORM conceptual modelling

NASA Astrophysics Data System (ADS)

Matula, Jiri; Belunek, Roman; Hunka, Frantisek

2017-07-01

Object Role Modelling methodology is the fact-based type of conceptual modelling. The aim of the paper is to emphasize a close connection to OWL documents and its possible mutual cooperation. The definition of entities or domain values is an indispensable part of the conceptual schema design procedure defined by the ORM methodology. Many of these entities are already defined in OWL documents. Therefore, it is not necessary to declare entities again, whereas it is possible to utilize references from OWL documents during modelling of information systems.

Flow-sediment-oyster interaction around degraded, restored, and reference oyster reefs in Florida's Indian River Lagoon

NASA Astrophysics Data System (ADS)

Kitsikoudis, V.; Kibler, K. M.; Spiering, D. W.

2017-12-01

This study analyzes flow patterns and sediment distributions around three oyster reefs in a bar-built estuarine lagoon. We studied a degraded reef, a recently restored reef, and a reference condition reef with a healthy live oyster community. The restored reef had been regraded and restored with oyster shell mats to aid in recruitment of oyster spat, with the goal of reestablishing a healthy oyster community. Despite the fact that flow-biota-sediment interaction constitutes a blossoming research field, actual field data are sparse and current knowledge emanates from flume studies and numerical modeling. Moreover, the hydraulic effect of restored oyster reefs has not been thoroughly investigated and it is not clear if the flow field and sediment erosion/deposition are similar or diverge from natural reefs. Instantaneous three-dimensional flow velocities were collected on reefs using a Nortek Vectrino Profiler and an acoustic Doppler current profiler (Nortek Aquadopp). The former measured a 2 - 3 cm velocity profile above the oyster bed, while the latter quantified incoming velocities across the flow profile approximately 10 m from the edge of the reef. Flow measurements were conducted during rising tides and are coupled with simultaneous wind speed and direction data. In addition, 20 cm deep sediment cores were retrieved on and off the investigated reefs. Sediment grain size distributions were determined after individual cores were processed for loss on ignition. Incoming flow velocities were as high as 10 cm/s, relatively higher than those recorded close to reefs. Mean and turbulent flow velocities close to the reefs, varied among the investigated sites, despite the similar wind flow conditions offshore. For instance, the measurements at the degraded reef showed decreased wave attenuation and augmented flow velocities compared to the other sites. Boat wakes exhibited a very distinct signal in the flow velocity time-series and significantly increased the approaching flow velocity at the reefs. The oyster roughness height at the restored reef (68 mm) was higher than the roughness at the reference reef (45 mm); however, the variance was higher at the latter. Sediments from degraded reef and the recently restored reef were coarser and contained less organic matter compared to the reference condition reef.

Reference Frames and Relativity.

ERIC Educational Resources Information Center

Swartz, Clifford

1989-01-01

Stresses the importance of a reference frame in mechanics. Shows the Galilean transformation in terms of relativity theory. Discusses accelerated reference frames and noninertial reference frames. Provides examples of reference frames with diagrams. (YP)

Approximate stresses in 2-D flat elastic contact fretting problems

NASA Astrophysics Data System (ADS)

Urban, Michael Rene

Fatigue results from the cyclic loading of a solid body. If the body subject to fatigue is in contact with another body and relative sliding motion occurs between these two bodies, then rubbing surface damage can accelerate fatigue failure. The acceleration of fatigue failure is especially important if the relative motion between the two bodies results in surface damage without excessive surface removal via wear. The situation just described is referred to as fretting fatigue. Understanding of fretting fatigue is greatly enhanced if the stress state associated with fretting can be characterized. For Hertzian contact, this can readily be done. Unfortunately, simple stress formulae are not available for flat body contact. The primary result of the present research is the development of a new, reasonably accurate, approximate closed form expression for 2-dimensional contact stresses which has been verified using finite element modeling. This expression is also combined with fracture mechanics to provide a simple method of determining when a crack is long enough to no longer be affected by the contact stress field. Lower bounds on fatigue life can then easily be calculated using fracture mechanics. This closed form expression can also be used to calculate crack propagation within the contact stress field. The problem of determining the cycles required to generate an initial crack and what to choose as an initial crack size is unresolved as it is in non-fretting fatigue.

Space Science

NASA Image and Video Library

2003-01-01

The Gravity Probe B (GP-B) is the relativity experiment developed at Stanford University to test two extraordinary predictions of Albert Einstein’s general theory of relativity. The experiment will measure, very precisely, the expected tiny changes in the direction of the spin axes of four gyroscopes contained in an Earth-orbiting satellite at a 400-mile altitude. So free are the gyroscopes from disturbance that they will provide an almost perfect space-time reference system. They will measure how space and time are very slightly warped by the presence of the Earth, and, more profoundly, how the Earth’s rotation very slightly drags space-time around with it. These effects, though small for the Earth, have far-reaching implications for the nature of matter and the structure of the Universe. This photograph is a close up of a niobium-coated gyroscope motor and its housing halves. GP-B is among the most thoroughly researched programs ever undertaken by NASA. This is the story of a scientific quest in which physicists and engineers have collaborated closely over many years. Inspired by their quest, they have invented a whole range of technologies that are already enlivening other branches of science and engineering. Launched April 20, 2004 , the GP-B program was managed for NASA by the Marshall Space Flight Center. Development of the GP-B is the responsibility of Stanford University along with major subcontractor Lockheed Martin Corporation. (Image credit to Don Harley.)

Comparative genomics analyses revealed two virulent Listeria monocytogenes strains isolated from ready-to-eat food.

PubMed

Lim, Shu Yong; Yap, Kien-Pong; Thong, Kwai Lin

2016-01-01

Listeria monocytogenes is an important foodborne pathogen that causes considerable morbidity in humans with high mortality rates. In this study, we have sequenced the genomes and performed comparative genomics analyses on two strains, LM115 and LM41, isolated from ready-to-eat food in Malaysia. The genome size of LM115 and LM41 was 2,959,041 and 2,963,111 bp, respectively. These two strains shared approximately 90% homologous genes. Comparative genomics and phylogenomic analyses revealed that LM115 and LM41 were more closely related to the reference strains F2365 and EGD-e, respectively. Our virulence profiling indicated a total of 31 virulence genes shared by both analysed strains. These shared genes included those that encode for internalins and L. monocytogenes pathogenicity island 1 (LIPI-1). Both the Malaysian L. monocytogenes strains also harboured several genes associated with stress tolerance to counter the adverse conditions. Seven antibiotic and efflux pump related genes which may confer resistance against lincomycin, erythromycin, fosfomycin, quinolone, tetracycline, and penicillin, and macrolides were identified in the genomes of both strains. Whole genome sequencing and comparative genomics analyses revealed two virulent L. monocytogenes strains isolated from ready-to-eat foods in Malaysia. The identification of strains with pathogenic, persistent, and antibiotic resistant potentials from minimally processed food warrant close attention from both healthcare and food industry.

The role of character displacement in the molarization of hominin mandibular premolars.

PubMed

Schroer, Kes; Wood, Bernard

2015-06-01

Closely related species are likely to experience resource competition in areas where their ranges overlap. Fossil evidence suggests that hominins in East Africa c. 2-1.5 million years ago may have lived synchronically and sympatrically, and that competition may have contributed to the different tooth sizes observed in Homo and Paranthropus. To assess the likelihood that these taxa overlapped, we applied a character displacement model to the postcanine tooth size of fossil hominins and validated this model in populations of living primates. Mandibular fourth premolar (P4 ) crown size was measured from fossil taxa and from living primate species where dietary overlap is established. Dimensions of the P4 crown were fitted to a character matrix and described as the response variables of a generalized linear model that took taxon and location as input variables. The model recovered significant divergence in samples of closely related, living primates. When applied to fossil hominins the same model detected strong indications of character displacement between early Homo and Paranthropus (P = 0.002) on the basis of their P4 crown size. Our study is an example of how ecologically informed morphologies measured in appropriate extant referents can provide a comparative context for assessing community and ecological evolution in the fossil record. © 2015 The Author(s). Evolution © 2015 The Society for the Study of Evolution.

A new rauisuchid (Archosauria, Pseudosuchia) from the Upper Triassic (Norian) of New Mexico increases the diversity and temporal range of the clade.

PubMed

Lessner, Emily J; Stocker, Michelle R; Smith, Nathan D; Turner, Alan H; Irmis, Randall B; Nesbitt, Sterling J

2016-01-01

Rauisuchids are large (2-6 m in length), carnivorous, and quadrupedal pseudosuchian archosaurs closely related to crocodylomorphs. Though geographically widespread, fossils of this clade are relatively rare in Late Triassic assemblages. The middle Norian (∼212 Ma) Hayden Quarry of northern New Mexico, USA, in the Petrified Forest Member of the Chinle Formation, has yielded isolated postcranial elements and associated skull elements of a new species of rauisuchid. Vivaron haydeni gen. et. sp. nov. is diagnosed by the presence of two posteriorly directed prongs at the posterior end of the maxilla for articulation with the jugal. The holotype maxilla and referred elements are similar to those of the rauisuchid Postosuchus kirkpatricki from the southwestern United States, but V. haydeni shares several maxillary apomorphies (e.g., a distinct dropoff to the antorbital fossa that is not a ridge, a straight ventral margin, and a well defined dental groove) with the rauisuchid Teratosaurus suevicus from the Norian of Germany. Despite their geographic separation, this morphological evidence implies a close phylogenetic relationship between V. haydeni and T. suevicus. The morphology preserved in the new Hayden Quarry rauisuchid V. haydeni supports previously proposed and new synapomorphies for nodes within Rauisuchidae. The discovery of Vivaron haydeni reveals an increased range of morphological disparity for rauisuchids from the low-paleolatitude Chinle Formation and a clear biogeographic connection with high paleolatitude Pangea.

Gravity Probe B Gyroscope Rotor

NASA Technical Reports Server (NTRS)

2003-01-01

The Gravity Probe B (GP-B) is the relativity experiment developed at Stanford University to test two extraordinary predictions of Albert Einstein's general theory of relativity. The experiment will measure, very precisely, the expected tiny changes in the direction of the spin axes of four gyroscopes contained in an Earth-orbiting satellite at a 400-mile altitude. So free are the gyroscopes from disturbance that they will provide an almost perfect space-time reference system. They will measure how space and time are very slightly warped by the presence of the Earth, and, more profoundly, how the Earth's rotation very slightly drags space-time around with it. These effects, though small for the Earth, have far-reaching implications for the nature of matter and the structure of the Universe. This photograph is a close up of a niobium-coated gyroscope motor and its housing halves. GP-B is among the most thoroughly researched programs ever undertaken by NASA. This is the story of a scientific quest in which physicists and engineers have collaborated closely over many years. Inspired by their quest, they have invented a whole range of technologies that are already enlivening other branches of science and engineering. Launched April 20, 2004 , the GP-B program was managed for NASA by the Marshall Space Flight Center. Development of the GP-B is the responsibility of Stanford University along with major subcontractor Lockheed Martin Corporation. (Image credit to Don Harley.)

Trajectory Control of Rendezvous with Maneuver Target Spacecraft

NASA Technical Reports Server (NTRS)

Zhou, Zhinqiang

2012-01-01

In this paper, a nonlinear trajectory control algorithm of rendezvous with maneuvering target spacecraft is presented. The disturbance forces on the chaser and target spacecraft and the thrust forces on the chaser spacecraft are considered in the analysis. The control algorithm developed in this paper uses the relative distance and relative velocity between the target and chaser spacecraft as the inputs. A general formula of reference relative trajectory of the chaser spacecraft to the target spacecraft is developed and applied to four different proximity maneuvers, which are in-track circling, cross-track circling, in-track spiral rendezvous and cross-track spiral rendezvous. The closed-loop differential equations of the proximity relative motion with the control algorithm are derived. It is proven in the paper that the tracking errors between the commanded relative trajectory and the actual relative trajectory are bounded within a constant region determined by the control gains. The prediction of the tracking errors is obtained. Design examples are provided to show the implementation of the control algorithm. The simulation results show that the actual relative trajectory tracks the commanded relative trajectory tightly. The predicted tracking errors match those calculated in the simulation results. The control algorithm developed in this paper can also be applied to interception of maneuver target spacecraft and relative trajectory control of spacecraft formation flying.

Closed loop control of penetration depth during CO₂ laser lap welding processes.

PubMed

Sibillano, Teresa; Rizzi, Domenico; Mezzapesa, Francesco P; Lugarà, Pietro Mario; Konuk, Ali Riza; Aarts, Ronald; Veld, Bert Huis In 't; Ancona, Antonio

2012-01-01

In this paper we describe a novel spectroscopic closed loop control system capable of stabilizing the penetration depth during laser welding processes by controlling the laser power. Our novel approach is to analyze the optical emission from the laser generated plasma plume above the keyhole, to calculate its electron temperature as a process-monitoring signal. Laser power has been controlled by using a quantitative relationship between the penetration depth and the plasma electron temperature. The sensor is able to correlate in real time the difference between the measured electron temperature and its reference value for the requested penetration depth. Accordingly the closed loop system adjusts the power, thus maintaining the penetration depth.

Indirect Identification of Linear Stochastic Systems with Known Feedback Dynamics

NASA Technical Reports Server (NTRS)

Huang, Jen-Kuang; Hsiao, Min-Hung; Cox, David E.

1996-01-01

An algorithm is presented for identifying a state-space model of linear stochastic systems operating under known feedback controller. In this algorithm, only the reference input and output of closed-loop data are required. No feedback signal needs to be recorded. The overall closed-loop system dynamics is first identified. Then a recursive formulation is derived to compute the open-loop plant dynamics from the identified closed-loop system dynamics and known feedback controller dynamics. The controller can be a dynamic or constant-gain full-state feedback controller. Numerical simulations and test data of a highly unstable large-gap magnetic suspension system are presented to demonstrate the feasibility of this indirect identification method.

Dustfall design of open coal yard in the power plant-a case study on the closed reconstruction project of coal storage yard in shengli power plant

NASA Astrophysics Data System (ADS)

Wang, Kunpeng; Ji, Weidong; Zhang, Feifei; Yu, Wei; Zheng, Runqing

2018-02-01

This thesis, based on the closed reconstruction project of the coal storage yard of Shengli Power Plant which is affiliated to Sinopec Shengli Petroleum Administration, first makes an analysis on the significance of current dustfall reconstruction of open coal yard, then summarizes the methods widely adopted in the dustfall of large-scale open coal storage yard of current thermal power plant as well as their advantages and disadvantages, and finally focuses on this project, aiming at providing some reference and assistance to the future closed reconstruction project of open coal storage yard in thermal power plant.

Closed Loop Control of Penetration Depth during CO2 Laser Lap Welding Processes

PubMed Central

Sibillano, Teresa; Rizzi, Domenico; Mezzapesa, Francesco P.; Lugarà, Pietro Mario; Konuk, Ali Riza; Aarts, Ronald; Veld, Bert Huis in 't; Ancona, Antonio

2012-01-01

In this paper we describe a novel spectroscopic closed loop control system capable of stabilizing the penetration depth during laser welding processes by controlling the laser power. Our novel approach is to analyze the optical emission from the laser generated plasma plume above the keyhole, to calculate its electron temperature as a process-monitoring signal. Laser power has been controlled by using a quantitative relationship between the penetration depth and the plasma electron temperature. The sensor is able to correlate in real time the difference between the measured electron temperature and its reference value for the requested penetration depth. Accordingly the closed loop system adjusts the power, thus maintaining the penetration depth. PMID:23112646

Plaque, caries level and oral hygiene habits in young patients receiving orthodontic treatment.

PubMed

Martignon, S; Ekstrand, K R; Lemos, M I; Lozano, M P; Higuera, C

2010-09-01

To assess plaque, caries, and oral hygiene habits amongst patients receiving fixed-orthodontic treatment at the Dental-Clinic, Universidad-El-Bosque, Bogotá, Colombia. Test-group: 74 12-29-year-olds receiving fixed-orthodontic treatment; reference-group: 63 12-29-year-olds before they started the orthodontic treatment. Visual examinations (one examiner) recorded the following: Ortho-plaque-Index (OPI) expressed per patient as good, fair and poor-oral-hygiene. Caries was scored with the modified-ICDAS-II criteria as: 0-sound; 1B/1W-brown/white-opacity-after-air-drying; 2B/2W-brown/white-opacity-without-air-drying; 3-microcavity; 4-underlying-shadow; 5/6-distinct/extensive-cavity. Filled/missing surfaces due-to-caries and caries-lesions on buccal surfaces at three sites around the brackets were recorded. A 7-item self-administered oral-hygiene habits' questionnaire was used. Chi-square test revealed that the oral-hygiene level was significantly better in the reference group compared to the test group (p < 0.05). The traditional mean DMF-S was 6.7 +/- 6.3 in the test- and 6.2 +/- 5.9 in the reference-group (p > 0.05). When adding modified-ICDAS-II lesions scores 1-4, the figure increased to 23.6 +/- 9.4 in the test- and to 13.6 +/- 10.3 in the reference-group (p < 0.001). A total of 96% had > or = 1 white-opacity in the test group versus 56% in the reference group (P < 0.001). In the test-group the buccal-surfaces accounted for most white-opacities and close to 1/3 of these lesions on the upper-anterior teeth were located around the brackets. The questionnaire disclosed that 58% in the test- vs. 44% in the reference-group did not accept having dental caries lesions during the orthodontic treatment. The results showed a high prevalence of white-opacities related to orthodontic appliances and indicate the need to implement preventive programmes at the dental clinic.

Saccharomyces cerevisiae: gene annotation and genome variability, state of the art through comparative genomics.

PubMed

Louis, Ed

2011-01-01

In the early days of the yeast genome sequencing project, gene annotation was in its infancy and suffered the problem of many false positive annotations as well as missed genes. The lack of other sequences for comparison also prevented the annotation of conserved, functional sequences that were not coding. We are now in an era of comparative genomics where many closely related as well as more distantly related genomes are available for direct sequence and synteny comparisons allowing for more probable predictions of genes and other functional sequences due to conservation. We also have a plethora of functional genomics data which helps inform gene annotation for previously uncharacterised open reading frames (ORFs)/genes. For Saccharomyces cerevisiae this has resulted in a continuous updating of the gene and functional sequence annotations in the reference genome helping it retain its position as the best characterized eukaryotic organism's genome. A single reference genome for a species does not accurately describe the species and this is quite clear in the case of S. cerevisiae where the reference strain is not ideal for brewing or baking due to missing genes. Recent surveys of numerous isolates, from a variety of sources, using a variety of technologies have revealed a great deal of variation amongst isolates with genome sequence surveys providing information on novel genes, undetectable by other means. We now have a better understanding of the extant variation in S. cerevisiae as a species as well as some idea of how much we are missing from this understanding. As with gene annotation, comparative genomics enhances the discovery and description of genome variation and is providing us with the tools for understanding genome evolution, adaptation and selection, and underlying genetics of complex traits.

Dual-Polarization Observations of Slowly Varying Solar Emissions from a Mobile X-Band Radar

PubMed Central

Gabella, Marco; Leuenberger, Andreas

2017-01-01

The radio noise that comes from the Sun has been reported in literature as a reference signal to check the quality of dual-polarization weather radar receivers for the S-band and C-band. In most cases, the focus was on relative calibration: horizontal and vertical polarizations were evaluated versus the reference signal mainly in terms of standard deviation of the difference. This means that the investigated radar receivers were able to reproduce the slowly varying component of the microwave signal emitted by the Sun. A novel method, aimed at the absolute calibration of dual-polarization receivers, has recently been presented and applied for the C-band. This method requires the antenna beam axis to be pointed towards the center of the Sun for less than a minute. Standard deviations of the difference as low as 0.1 dB have been found for the Swiss radars. As far as the absolute calibration is concerned, the average differences were of the order of −0.6 dB (after noise subtraction). The method has been implemented on a mobile, X-band radar, and this paper presents the successful results that were obtained during the 2016 field campaign in Payerne (Switzerland). Despite a relatively poor Sun-to-Noise ratio, the “small” (~0.4 dB) amplitude of the slowly varying emission was captured and reproduced; the standard deviation of the difference between the radar and the reference was ~0.2 dB. The absolute calibration of the vertical and horizontal receivers was satisfactory. After the noise subtraction and atmospheric correction a, the mean difference was close to 0 dB. PMID:28531164

Dual-Polarization Observations of Slowly Varying Solar Emissions from a Mobile X-Band Radar.

PubMed

Gabella, Marco; Leuenberger, Andreas

2017-05-22

The radio noise that comes from the Sun has been reported in literature as a reference signal to check the quality of dual-polarization weather radar receivers for the S-band and C-band. In most cases, the focus was on relative calibration: horizontal and vertical polarizations were evaluated versus the reference signal mainly in terms of standard deviation of the difference. This means that the investigated radar receivers were able to reproduce the slowly varying component of the microwave signal emitted by the Sun. A novel method, aimed at the absolute calibration of dual-polarization receivers, has recently been presented and applied for the C-band. This method requires the antenna beam axis to be pointed towards the center of the Sun for less than a minute. Standard deviations of the difference as low as 0.1 dB have been found for the Swiss radars. As far as the absolute calibration is concerned, the average differences were of the order of -0.6 dB (after noise subtraction). The method has been implemented on a mobile, X-band radar, and this paper presents the successful results that were obtained during the 2016 field campaign in Payerne (Switzerland). Despite a relatively poor Sun-to-Noise ratio, the "small" (~0.4 dB) amplitude of the slowly varying emission was captured and reproduced; the standard deviation of the difference between the radar and the reference was ~0.2 dB. The absolute calibration of the vertical and horizontal receivers was satisfactory. After the noise subtraction and atmospheric correction a, the mean difference was close to 0 dB.