Alvarez Garcia, D M; Pérez-Hérazo, A; Amat, E
The life history traits of blow fly Cochliomyia macellaria (Fabricius, 1775) was studied under semi-controlled laboratory conditions at 29.14°C temperature, 72.53% relative humidity, and 12-h photoperiod. The raw data were analyzed based on the age-stage, two-sex life table, considering the development rates among individuals of both sexes. Cochliomyia macellaria survival rate was 0.43 (♂) and 0.40 (♀), while life expectancy was 17.9 (♂) and 20.9 (♀) days, for adult males and females, respectively. The total fecundity was 681.15 eggs/female, with an average of 3.65 batches/female and 199 eggs/batch. The intrinsic rate of increase (r) was 0.327 days(-1), the finite rate of population increase (λ) was 3.35 days(-1), the mean generation time (T) was 17.15 days, and the net reproduction rate (R 0 ) was 272.46 offspring/individual. The population parameters found here corroborates that C. macellaria population act as a r selected species under laboratory conditions. Additionally, development data and accumulated degree days (ADD) for each stage of C. macellaria are provided and its implications for the forensic use are discussed.
Larvae of the secondary screwworm, Cochliomyia macellaria (Fabricius), feed on carrion and may sometimes cause animal myiasis. They have been reared in the laboratory on various animal tissues to study their growth and development because of their importance in forensic science. We use the secondary...
The secondary screwworm, Cochliomyia macellaria (F.), is an important blowfly species affecting both livestock and humans. It can transmit pathogenic disease agents mechanically and is an agent of facultative myiasis, which leads to economic losses. The adult flies are attracted to decomposing carca...
Volatile chemicals from waste artificial larval media as well as from bovine blood inoculated with bacteria isolated from screwworm-infested wounds attract gravid females of Cochliomyia hominivorax Coquerel and C. macellaria (F.). Chemicals previously identified from volatiles are dimethyl disulfide...
Mendonça, Paloma Martins; Barbosa, Rodrigo Rocha; Cortinhas, Lucas Barbosa; dos Santos-Mallet, Jacenir Reis; de Carvalho Queiroz, Margareth Maria
Cochliomyia macellaria (Diptera: Calliphoridae) is known as the secondary screwworm because it causes secondary or facultative myiasis when the larvae feed on necrotic tissues. This fly has a significant medical and veterinary importance since it has been reported to transport eggs of Dermatobia hominis (human botfly), which can cause significant economic losses to livestock. Since this screwworm has been collected colonizing both pig carcasses and human cadavers, it is considered one of the most important species for forensic entomology studies. Scanning electron microscopy (SEM) gives detailed information on the morphological characteristics which can help identify the immature forms of the flies. The aim of this study was to describe and analyze the morphological characteristics of the eggs, all the larval instars, and the puparia of Cochliomyia macellaria using SEM. The egg is ellipsoid and the dorsal surface is concave. The islands inside the median area had no anastomosis, but some perforations could be observed. From the second larval instar onwards, besides the intersegmental spines, other bands of spines were observed at the abdominal segments. Two spiracular openings were visible on the first and second larval instars, which were not expected. These characteristics are specific to Cochliomyia genus. The number and the general aspect of the spine tips in the cephalic region, the intersegmental bands on the abdomen, and the number of the spiracular openings could together help identify C. macellaria.
Paulo, D F; Azeredo-Espin, A M L; Canesin, L E C; Vicentini, R; Junqueira, A C M
MicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression through post-transcriptional regulation. Here, we report the identification and characterization of miRNAs in two closely related screwworm flies with different feeding habits: Cochliomyia hominivorax and Cochliomyia macellaria. The New World screwworm, C. hominivorax, is an obligatory parasite of warm-blooded vertebrates, whereas the secondary screwworm, C. macellaria, is a free-living organism that feeds on decaying organic matter. Here, the small RNA transcriptomes of adults and third-instar larvae of both species were sequenced. A total of 110 evolutionarily conserved miRNAs were identified, and 10 putative precursor miRNAs (pre-miRNAs) were predicted. The relative expression of six selected miRNAs was further investigated, including miRNAs that are related to reproduction and neural processes in other insects. Mature miRNAs were also characterized across an evolutionary time scale, suggesting that the majority of them have been conserved since the emergence of the Arthropoda [540 million years ago (Ma)], Hexapoda (488 Ma) and Brachycera (195 Ma) lineages. This study is the first report of miRNAs for screwworm flies. We also performed a comparative analysis with the hereby predicted miRNAs from the sheep blowfly, Lucilia cuprina. The results presented may advance our understanding of parasitic habits within Calliphoridae and assist further functional studies in blowflies.
Li, Fang; Vensko, Steven P.; Belikoff, Esther J.; Scott, Maxwell J.
Transformer (TRA) promotes female development in several dipteran species including the Australian sheep blowfly Lucilia cuprina, the Mediterranean fruit fly, housefly and Drosophila melanogaster. tra transcripts are sex-specifically spliced such that only the female form encodes full length functional protein. The presence of six predicted TRA/TRA2 binding sites in the sex-specific female intron of the L. cuprina gene suggested that tra splicing is auto-regulated as in medfly and housefly. With the aim of identifying conserved motifs that may play a role in tra sex-specific splicing, here we have isolated and characterized the tra gene from three additional blowfly species, L. sericata, Cochliomyia hominivorax and C. macellaria. The blowfly adult male and female transcripts differ in the choice of splice donor site in the first intron, with males using a site downstream of the site used in females. The tra genes all contain a single TRA/TRA2 site in the male exon and a cluster of four to five sites in the male intron. However, overall the sex-specific intron sequences are poorly conserved in closely related blowflies. The most conserved regions are around the exon/intron junctions, the 3′ end of the intron and near the cluster of TRA/TRA2 sites. We propose a model for sex specific regulation of tra splicing that incorporates the conserved features identified in this study. In L. sericata embryos, the male tra transcript was first detected at around the time of cellular blastoderm formation. RNAi experiments showed that tra is required for female development in L. sericata and C. macellaria. The isolation of the tra gene from the New World screwworm fly C. hominivorax, a major livestock pest, will facilitate the development of a “male-only” strain for genetic control programs. PMID:23409170
Li, Fang; Vensko, Steven P; Belikoff, Esther J; Scott, Maxwell J
Transformer (TRA) promotes female development in several dipteran species including the Australian sheep blowfly Lucilia cuprina, the Mediterranean fruit fly, housefly and Drosophila melanogaster. tra transcripts are sex-specifically spliced such that only the female form encodes full length functional protein. The presence of six predicted TRA/TRA2 binding sites in the sex-specific female intron of the L. cuprina gene suggested that tra splicing is auto-regulated as in medfly and housefly. With the aim of identifying conserved motifs that may play a role in tra sex-specific splicing, here we have isolated and characterized the tra gene from three additional blowfly species, L. sericata, Cochliomyia hominivorax and C. macellaria. The blowfly adult male and female transcripts differ in the choice of splice donor site in the first intron, with males using a site downstream of the site used in females. The tra genes all contain a single TRA/TRA2 site in the male exon and a cluster of four to five sites in the male intron. However, overall the sex-specific intron sequences are poorly conserved in closely related blowflies. The most conserved regions are around the exon/intron junctions, the 3' end of the intron and near the cluster of TRA/TRA2 sites. We propose a model for sex specific regulation of tra splicing that incorporates the conserved features identified in this study. In L. sericata embryos, the male tra transcript was first detected at around the time of cellular blastoderm formation. RNAi experiments showed that tra is required for female development in L. sericata and C. macellaria. The isolation of the tra gene from the New World screwworm fly C. hominivorax, a major livestock pest, will facilitate the development of a "male-only" strain for genetic control programs.
Yusseff-Vanegas, Sohath; Agnarsson, Ingi
Abstract Cochliomyia Townsend includes several abundant and one of the most broadly distributed, blow flies in the Americas, and is of significant economic and forensic importance. For decades, Cochliomyia hominivorax (Coquerel) and Cochliomyia macellaria (Fabricius) have received attention as livestock parasites and primary indicator species in forensic entomology. However, Cochliomyia minima Shannon and Cochliomyia aldrichi Del Ponte have only been subject to basic taxonomy and faunistic studies. Here we present the first complete phylogeny of Cochliomyia including numerous specimens per species, collected from 13 localities in the Caribbean. Four genes, the mitochondrial COI and the nuclear EF-1α, 28S rRNA, and ITS2, were analyzed. While we found some differences among gene trees, a concatenated gene matrix recovered a robustly supported monophyletic Cochliomyia with Compsomyiops Townsend as its sister group and recovered the monophyly of Cochliomyia hominivorax, Cochliomyia macellaria and Cochliomyia minima. Our results support a close relationship between Cochliomyia minima and Cochliomyia aldrichi. However, we found Cochliomyia aldrichi containing Cochliomyia minima, indicating recent speciation, or issues with the taxonomy of the group. We provide basic information on habitat preference, distribution and feeding habits of Cochliomyia minima and Cochliomyia aldrichi that will be useful for future forensic studies in the Caribbean. PMID:27563274
Flores, Micah; Crippen, Tawni L; Longnecker, Michael; Tomberlin, Jeffery K
Forensic entomologists often rely on development data associated with a given species to estimate when it colonized human or other vertebrate remains. In most instances, these development studies are based on single species reared in isolation in the laboratory. This study examined the impact of excretions and secretions (ES) associated with third-instar Chrysomya rufifacies (Macquart), a predator, on the development of its prey, Cochliomyia macellaria (F.). Not surprisingly, Ch. rufifacies ES did not impact the development of first- or second-instar C. macellaria, which are typically not preyed on by Ch. rufifacies. However, development of third-instar C. macellaria, which do experience predation, was impacted. First, larvae were longer than those in the control (deionized water, dH2O). Filtering the ES and removing the associated bacteria and byproducts >0.2 µm dampened the previous impact observed by the unfiltered ES on third-instar C. macellaria. Second, third-instar C. macellaria treated with unfiltered ES completed pupariation 8 h quicker than the controls. Filtering the ES lessened this effect by 50%. And finally, third-instar C. macellaria treated with filtered or unfiltered Ch. rufifacies ES reached adulthood ∼5 h faster than controls treated with dH2O. In summary, these data have large ramifications for forensic entomology, as multiple species being present on decomposing remains is not uncommon. Understanding the impact of associated ES produced by interspecific cohorts on associated development could lead to more precise estimates of the minimum postmortem interval for forensic investigation of decomposing remains. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: firstname.lastname@example.org.
Nassu, Mariana Prado; Thyssen, Patricia Jacqueline
Larval therapy (LT) is the application of carrion flies (Diptera) sterile larvae on chronic or infected wounds to promote or accelerate the healing process. High cost and the development of resistance by certain groups of pathogenic bacteria to these drugs encouraged the resurgence of LT, currently used in approximately 20 countries and more recently in Brazil. This study evaluated the behavior and larval density of Cochliomyia macellaria F. (Calliphoridae), one of the most appropriate species for debridement of injuries with necrotic tissue. Tegumentar lesions were induced in Wistar rats by subcutaneously application of 0.2 ml of a 1:4 hydrochloric acid and sterile distilled water in the dorsal region. Five experimental groups were set up: (LT 5) treatment with 5 larvae/cm(2); (LT 15) 15 larvae/cm(2); (LT 25) 25 larvae/cm(2); (DEB) mechanical debridement, and (NUL) animals that did not receive any treatment. In the LT groups, larvae used were sterilized with sodium hypochlorite (NaClO) and maintained for 12 h in the lesions. The healing process was assessed qualitatively (macroscopically and microscopically) and quantitatively (time interval to complete healing). It was observed that the immature fed only on necrotic tissue, thus C. macellaria is an excellent candidate for use in LT. There was no significant difference in healing time between experimental groups. However, it was observed that in LT 25, there was greater vascularization in tissues when compared to the other treatments. The mechanisms involved in this process are unknown, but it is evident that the larvae have an important role in modulating the host immune response. It is essential that future applications of larval therapy consider using a higher density of larvae (minimum of 25 larvae/cm(2)) than is currently recommended.
Skoda, Steven R.; Figarola, James L.; Pornkulwat, Saowaluck; Foster, John E.
The screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), is one of the most devastating arthropod pests of livestock in the Western Hemisphere. Early instars are very difficult to distinguish morphologically from several closely related blow fly species. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers were developed for identifying C. hominivorax from other wound inhabiting species. Forty decameric primers were screened; nine showed clear reproducible RAPD profiles suitable for distinguishing all life stages of C. hominivorax from 7 other species, including C. macellaria (Fabricius). The results from RAPD-PCR with field-collected samples of unknown first instars agreed with morphological identification that the samples were not C. hominivorax. Three different primers showed DNA polymorphisms (intraspecific) for samples originating from Mexico, Costa Rica, Panama, Jamaica, and Brazil. Therefore, RAPD-PCR may be useful for determining the geographic origin of C. hominivorax samples. Comparing products from these primers, used with known and unknown screwworm samples from an outbreak in Mexico, clearly showed that the outbreak did not originate from the mass rearing facility. Accurate identification of suspected C. hominivorax samples is possible using RAPD-PCR. Further development to identify the geographic origin of samples would benefit the ongoing surveillance programs against C. hominivorax and the decision process during suspected outbreaks of this important pest. PMID:24219502
Florez, Eliana; Wolff, Marta
Larvae of 13 blowfly species from Colombia are described and an illustrated key for all them is presented. All larval instars of Calliphora nigribasis Macquart, Cochliomyia macellaria (Fabricius), Chrysomya albiceps (Wiedemann), Chrysomya megacephala (Fabricius), Hemilucilia segmentaria (Fabricius), Hemilucilia semidiaphana (Rondani), Lucilia eximia (Weidemann) are described, but the second and third instars of Compsomyiops verena (Walter), and only the third instar of Calliphora vicina Robineau-Desvoidy, Cochliomyia hominivorax (Coquerel), Lucilia peruviana Robineau-Desvoidy, Lucilia sericata (Meigen) and Sarconesiopsis magellanica (Le Guillou).
Forensic entomologists often rely on development data associated with a given species to estimate when it colonized human or other vertebrate remains. In most instances, these development studies are based on single species reared in isolation in the laboratory. This study examined the impact of e...
The New World Screwworm (NWS), Cochliomyia hominivorax, is a pest insect that is endemic to subtropical and tropical regions of the Western Hemisphere. The female lays eggs in open wounds or orifices of warm-blooded animals. Upon hatching, the resulting larvae feed upon the host's living tissues, wh...
Deciphering the mechanisms that regulate animal behavior related to succession on ephemeral resources is critical for elucidating food web dynamics and nutrient recycling. Blow fly (Diptera: Calliphoridae) colonization and utilization of vertebrate carrion serve as a model for such studies, as the...
Calderón H, Perla; Rojas E, Camilo; Apt B, Werner; Castillo O, Douglas
Myiasis is an infestation of tissues and organs of humans and animals by Diptera larvae (flies, horseflies, mosquitoes). They are located at different body sites, and classified clinically as cutaneous, visceral and cavitary. We report a 26-year-old woman with a history of seborrheic dermatitis and recent trip to Brazil. She presented with a seven days history of suppurating wounds in the parieto-occipital area of the scalp. At physical examination we found three ulcers of approximately 1.5 cm each, with multiple mobile larvae inside. The obtained larvae were analyzed, identifying Cochliomyia hominivorax larvae at L2 and L3 stages. The patient was managed successfully with oral antimicrobials and local cleansing. The screwworm Cochliomyia hominivorax in our country is rare. Known risk factors are wounds, poor personal hygiene, extreme ages, psychiatric disorders, presence of specific dermatosis such as psoriasis and seborrheic dermatitis, among others.
Tantawi, Tarek I; Sinclair, Bradley J
Seven species of Calliphoridae are reported from the Galápagos Islands, Ecuador: Lucilia pionia (Walker), L. setosa (James), L. deceptor (Curran), L. eximia (Wiedemann), Cochliomyia macellaria (Fabricius), Chrysomya albiceps (Wiedemann), and Chrysomya megacephala (Fabricius). Lucilia eximia is newly recorded from the islands. Lucilia sp. near pionia is recorded from the island of Española. The distribution and collection records of these species are discussed and listed, and a key to their identification is provided. Chrysomya rufifacies (Macquart) is reported for the first time from mainland Ecuador and the identification of this species is outlined.
Trombetta, Luis; Oliva, Adriana; Galache, Viviana; Bava, Javier; Troncoso, Alcides
Myiasis is the condition resulting from the invasion of tissues or organs of man or animals by dipterous larvae. The blowflies (Calliphoridae) of Argentina comprise several species that may cause myiasis by colonizing wounds or infected body orifices, and one specific parasite: Cochliomyia hominivorax. This species often causes traumatic myiasis in cattle, dogs and cats, and it is not rare in humans. The larvae consume living tissues, so they are dangerous unless speedily removed. Immediate operative exploration along with the removal of larvae and primary defect closure is recommended in every case. Here we report a case of myiasis in a scalp wound caused by blunt force trauma to the area, in a male patient with a case history of alcohol and drug abuse. Seventy-one living larvae were extracted from the wound and determined as C. hominivorax in the Forensic Entomology Laboratory. Given the aggressiveness of these larvae, specific and quick diagnosis as well as the application of appropriate treatment is crucial.
Bendele, Kylie G; Guerrero, Felix D; Cameron, Connor; Perez de Leon, Adalberto A
The New World Screwworm (NWS), Cochliomyia hominivorax, is a pest insect that is endemic to subtropical and tropical regions of the Western Hemisphere. The female lays eggs in open wounds or orifices of warm-blooded animals. Upon hatching, the resulting larvae feed upon the host׳s living tissues, which can become infected and death can occur. The sterile insect technique was developed to eradicate this pest from North America and new female conditional-lethal strains that generate only male individuals are being developed for use in the eradication program. To facilitate the identification of useful transcripts and gene promoters for these new strains, we used an Illumina Hi-Seq protocol to sequence the testes transcriptome of NWS. We report the assembly of 4149 transcripts (≥200 nt) from testes dissected from NWS males obtained from the J06 strain used in the screwworm production plant in Pacora, Panama. Functional annotation resulted in 2060, 2031, 558, and 325 transcripts with assigned BlastX, Gene Ontology, Enzyme Codes, and KEGG pathway information, respectively. In the Gene Ontology annotations, 6% and 3% of the transcripts in the Biological Process Ontology were noted as Developmental Process and Reproduction, respectively. This data set will serve as a resource to facilitate studies of sex determination in the NWS and the development of recombinant vectors that can be used to create new male-only strains of NWS.
Cunningham, E P; Abusowa, M; Lindquist, D A; Sidahmed, A E; Vargas-Terán, M
The New World Screwworm (NWS, Cochliomyia hominivorax) is an obligate parasite of warm-blooded animals. The female lays up to 300 eggs in any break in the skin, and the resulting larvae (screwworms) burrow into surrounding living flesh. Infested animals frequently die, while the annual cost of controlling the pest in domestic animals is about US $10 per head. NWS is endemic in tropical Latin America. In 1988, it was detected in Libya, presumable introduced with imported sheep. By 1990, the infestation had spread to an area of 25,000 km2 containing some 2 million livestock. In early 1991, an internationally funded eradication programme was undertaken by FAO, using sterile insects. Each week, 40 million pupae were flown from a production plant in Mexico, and the emerged adults were distributed by over the infested area. Within a few months, the infestation has been eradicated. Whereas 12,000 infested animals were found in 1990, only 6 were detected in 1991. The programme involved the shipping and distribution of 1.3 billion sterile insects, animal inspections totalling 40 million and laboratory examination of 280,000 trapped flies. While the programme cost close to US $75 million, a benefit/cost ration of 50:1 has been estimated.
The New World screwworm, Cochliomyia hominivorax, was a devastating pest of livestock and other animals, including humans, throughout the US, Mexico and Central America. Although eradicated from North America, the screwworm still is a pest in South America and the Caribbean. Reinfestation of North A...
We used an expressed sequence tag and 454 pyrosequencing approach to initiate a study of the genome of the New World Screwworm, Cochliomyia hominivorax (Coquerel). Two normalized cDNA libraries were constructed from RNA isolated from embryos and 2nd instar larvae from the Panama 95 strain. Approxima...
The New World screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), has been eradicated from North and Central America using the sterile insect technique. This success has been based on mass production of high quality screwworms using artificial diets since 1958. Many diet formulat...
In a collaboration with National Center for Genome Resources researchers, we sequenced and assembled the testes transcriptome derived from the Pacora, Panama, production plant strain of the New World Screwworm, Cochliomyia hominivorax. This transcriptome contains 4,149 unigenes and the Transcriptome...
In a collaboration with National Center for Genome Resources researchers, we sequenced and assembled the testes transcriptome derived from the Pacora, Panama, production plant strain J06 of the New World Screwworm, Cochliomyia hominivorax. This sequencing project produced 72,750,822 raw reads and th...
Lutheran pastor and astronomer in Osteel, East Frisia (northwest Germany), discoverer (1596) of the first known variable star, mira stella (`wonderful star'), now simply Mira (Omicron Ceti). Fabricius observed the star at its brightest and thought it was a nova, after which Holwarda noticed that a star in Cetus cataloged by PTOLEMY and TYCHO was missing but then it reappeared. Eventually the long...
Batista-da-Silva, José A.; Borja, Gonzalo E. M.; Queiroz, Margareth M. C.
Myiasis is the infestation of living vertebrates by fly larvae that feed for at least part of their development on the host's dead or living tissues, body substances, or ingested food. The occurrences of traumatic myiasis in humans and animals in urban and rural environments represent serious economic and public health concerns. This study reports a 49-year-old tracheostomized man undergoing chemotherapy treatment who was parasitized in the hospital in São Gonçalo, Rio de Janeiro, Brazil, by larvae of the screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in the thoracic cavity. PMID:22243525
Pradel model mark, release, recapture estimates of survivorship, recruitment, and the rate of density-independent population growth, are presented for eight mark-recapture studies of the screwworm Cochliomyia hominivorax from Costa Rica, totaling 19,714 released and 4,504 recaptured flies. Corrobor...
Swiger, S L; Hogsette, J A; Butler, J F
Larval interactions of dipteran species, blow flies in particular, were observed and documented daily over time and location on five black bear carcasses in Gainesville, FL, USA, from June 2002 - September 2004. Cochliomyia macellaria (Fabricius) or Chrysomya megacephala (Fabricius) larvae were collected first, after which Chrysomya rufifacies (Macquart) oviposited on the carcasses in multiple locations (i.e., neck, anus, and exposed flesh) not inhabited already by the other blow fly larvae. Within the first week of decomposition, C. rufifacies larvae grew to ≥12 mm, filling the carcasses with thousands of larvae and replacing the other calliphorid larvae either through successful food source competition or by predation. As a result, C. macellaria and C. megacephala were not collected past their third instar feeding stage. The blow fly species, C. megacephala, C. macellaria, Lucilia caeruleiviridis (Macquart), Phormia regina (Meigen), Lucilia sericata (Meigen), and C. rufifacies, completed two developmental cycles in the 88.5-kg carcass. This phenomenon might serve to complicate or prevent the calculation of an accurate postmortem interval.
Galindo, Luciane A; Moral, Rafael A; Moretti, Thiago C; Godoy, Wesley A C; Demétrio, Clarice G B
The objective of the present study was to determine whether blow flies (Diptera: Calliphoridae) are able to identify larvae of an intraguild predator species in the substrate and avoid laying eggs there. Blow flies oviposited in traps with different treatments: substrate only and substrate with larvae of Chrysomya albiceps (Wiedemann, 1819), Chrysomya megacephala (Fabricius, 1794), or Chrysomya putoria (Wiedemann, 1830). Ch. megacephala, Ch. putoria, and Lucilia eximia (Wiedemann, 1819) avoided laying eggs in the trap containing Ch. albiceps larvae. Cochliomyia macellaria (Fabricius, 1775) did not oviposit differently in each substrate but had overall low abundance. The prevalence of species on corpses may be influenced by the ability of the species to detect the presence of other species, mainly predators. In this sense, intraguild predation may result in misinterpretations of a crime scene and should be considered when assessing the minimum postmortem interval.
Alonso-Zarazaga, Miguel A
The types of thirty-two nominal weevil species described by Johann Christian Fabricius are reviewed and lecto- and paralectotypes are designated for twenty-two of them. A neotype is designated for Curculiosticticus Fabricius, 1777. Protapionvaripes (Germar, 1817) is declared a nomen protectum over Curculioflavipes Fabricius, 1775. Based on a study of syntypes, Rhinomacercurculioides Fabricius, 1781 is confirmed as a member of Mycterus (Mycteridae), Bruchusundatus Fabricius, 1787 is tentatively transferred to Erotylidae, Curculiofulvirostris Fabricius, 1787 and Anthribusroboris Fabricius, 1798 are confirmed as members of Salpingus (Salpingidae), and Brachyceruscristatus Fabricius, 1798 is transferred to Tenebrionidae. Based on lectotype designation, Curculiocaninus Fabricius, 1792 is confirmed as a synonym of Sitonalineatus (Linnaeus, 1758) and Curculioinnocuus Fabricius, 1802 as a synonym of Cneorhinusbarcelonicus (Herbst, 1797). Bruchusrufipes Fabricius, 1792 is not considered an available species name, but a later use of Bruchusrufipes Olivier, 1790. Cossonusincisus Pascoe, 1885 is reinstated as valid from synonymy under Cossonusilligeri Champion, 1909 and Cossonusvulneratus Illiger, 1805 from synonymy under Cossonuscanaliculatus (Fabricius, 1792) (a primary homonym of Curculiocanaliculatus Olivier, 1791). Cossonuscanaliculatus Fabricius, 1802 is a secondary homonym of the former and is replaced with Cossonusincisus. Salpingusfulvirostris (Fabricius, 1787) is reinstated as valid from synonymy under Salpingusplanirostris (Fabricius, 1787), a primary homonym of Curculioplanirostris Piller & Mitterpacher, 1783. The following new combinations are proposed: Brachysomuserinaceus (Fabricius, 1802) (from Curculio), Bronchusferus (Gyllenhal, 1840) (from Hipporhinus), Bronchusglandifer (Fabricius, 1792) (from Curculio), Bronchusnivosus (Sparrman, 1785) (from Curculio), Bronchussparrmani (Gyllenhal, 1833) (from Hipporhinus), Coelocephalapionatrirostre (Fabricius, 1802
Declan J. Fallon; Leellen F. Solter; Leah S. Bauer; Deborah L. Miller; James R. Cate; Michael L. McManus
Entomopathogenic nematodes were screened for efficacy against the cottonwood borer, Plectrodera scalator (Fabricius). Steinernema feltiae SN and S. carpocapsae All killed 58 and 50% of larvae, respectively, in Wlter paper bioassays but less than 10% in diet cup bioassays. S. glaseri NJ, S. riobrave TX, and H. indica MG-13 killed less than 10% of larvae in both assays....
Coronado, A; Kowalski, A
The geographical distribution and seasonality of the New World screwworm (NWS), Cochliomyia hominivorax Coquerel (Diptera: Calliphoridae), were monitored through the use of sentinel animals as part of a co-ordinated programme involving veterinarians and farmers, as well as undergraduate students and teachers from veterinary colleges in Venezuela. This surveillance activity made it possible to collect NWS egg masses or larvae from all 23 states in the country and to determine that the rainy season has a strong positive influence on the number of cases of myiasis caused by C. hominivorax in dogs. In addition, efforts were made to obtain the co-operation of the public health service in order to document the extent of human myiasis in the western-central region of Venezuela. Preliminary results revealed 241 cases over a 7-year period, with cases reported in infants as well as in elderly people. Larvae causing myiasis, other than C. hominivorax, were collected from primary myiasis in rabbit (Lucilia eximia [Wiedemann]), dog (an unidentified sarcophagid species), birds (Philornis sp.) and wild mice (Cuterebra sp.). The economic impact of NWS in Venezuela has not been calculated in terms of loss of milk and meat production, damage to hides or death of animals. Control costs (e.g. cost of larvicides) have been estimated at US$ 2 m per year. Control of myiasis in animals is achieved through the use of chemical compounds, mainly organophosphorus (OP) compounds, macrocyclic lactones and, more recently, a foamy spray based on spinosad. Concerns about insecticide resistance to OP compounds have been raised.
Reck, José; Marks, Fernanda S; Rodrigues, Rogério O; Souza, Ugo A; Webster, Anelise; Leite, Romário C; Gonzales, João Carlos; Klafke, Guilherme M; Martins, João Ricardo
The larval phase of Cochliomyia hominivorax (screwworm) is an obligate parasite of vertebrate animals, particularly mammals, and widespread in South America, where it remains one of the most important parasitic diseases of domestic animals. The skin of cattle highly infested by ticks, with cutaneous lesions, exudation of tissue fluid and blood scent seems to produce the ideal environment for fly attraction. However, an association between these parasites was never investigated. The aim of this work was to verify if there is an association between Rhipicephalus microplus tick load and the occurrence of C. hominivorax myiasis in cattle, and to quantify the risk. Sixty bovine (Bos taurus taurus, Angus breed) under field conditions were observed for 24 weeks, during which weekly tick counts and examination for the presence of myiasis were performed. There was a significant association between a high tick burden (24-week mean above 50 ticks per animal) and myiasis occurrence (P=0.0102). The calculated relative risk (RR) for C. hominivorax myiasis occurrence in cattle with high tick burden was 3.85 (CI95%=1.23-12.13); indicating that cattle highly parasitized by R. microplus have about four times more risk of myiasis than those with a low parasite load. As far as we aware, this is the first statistically based evidence of the relationship between R. microplus parasitic load and occurrence of myiasis by C. hominivorax. This result could be useful for the design of integrated control strategies for these parasites and to provide more information for the understanding of cattle tick parasitism in cattle production. Copyright © 2013 Elsevier B.V. All rights reserved.
Transporting live screwworms Cochliomyia hominivorax Coquerel for developing new strains from countries where foot-and-mouth disease (FMD) and classical swine fever (CSF) are endemic, to the mass rearing facilities in Mexico and Panama may introduce these exotic diseases. This study was conducted to...
Barreto, Mauricio; Burbano, María Elena; Barreto, Pablo
Adult specimens of Cochliomyia macellaria, Chrysomya megacephala, Ch. rufifacies, Lucilia sp. (Calliphoridae), Musca domestica (Muscidae), Oxelytrum discicolle (Silphidae) and Sarcophagidae were recovered from 12 human cadavers in Cali, Valle, Colombia. Information regarding these findings is presented.
de la Ossa, Napoleón; Castro, Luis Eduardo; Visbal, Lila; Santos, Ana María; Díaz, Esther; Romero-Vivas, Claudia M E
Human myiasis is the parasitism of human tissues by fly larvae. Diagnoses are based on clinical pattern of tissue damage and presence of insect stages. Herein, a case myiasis is described in a seven-year-old female child. She presented with fever associated with abscessed scalp lesions containing exposed larvae. Severe pediculosis was also observed. The patient was hospitalized and treated with clindamycin, gentamicin (for bacterial secondary infections) and ivermectin (treatment for lice) after which the patient showed clinical improvement and was discharged four days later. Since human myiasis can be caused by a number of different species, larvae were collected from the patient and identified as those of Cochliomyia hominivorax (Diptera: Calliphoridae). Because other cases of coinfestation of flies and lice are on record, health workers are to be alerted about the possible pediculosis-myasis risk.
Alonso-Zarazaga, Miguel A.
Abstract The types of thirty-two nominal weevil species described by Johann Christian Fabricius are reviewed and lecto- and paralectotypes are designated for twenty-two of them. A neotype is designated for Curculio sticticus Fabricius, 1777. Protapion varipes (Germar, 1817) is declared a nomen protectum over Curculio flavipes Fabricius, 1775. Based on a study of syntypes, Rhinomacer curculioides Fabricius, 1781 is confirmed as a member of Mycterus (Mycteridae), Bruchus undatus Fabricius, 1787 is tentatively transferred to Erotylidae, Curculio fulvirostris Fabricius, 1787 and Anthribus roboris Fabricius, 1798 are confirmed as members of Salpingus (Salpingidae), and Brachycerus cristatus Fabricius, 1798 is transferred to Tenebrionidae. Based on lectotype designation, Curculio caninus Fabricius, 1792 is confirmed as a synonym of Sitona lineatus (Linnaeus, 1758) and Curculio innocuus Fabricius, 1802 as a synonym of Cneorhinus barcelonicus (Herbst, 1797). Bruchus rufipes Fabricius, 1792 is not considered an available species name, but a later use of Bruchus rufipes Olivier, 1790. Cossonus incisus Pascoe, 1885 is reinstated as valid from synonymy under Cossonus illigeri Champion, 1909 and Cossonus vulneratus Illiger, 1805 from synonymy under Cossonus canaliculatus (Fabricius, 1792) (a primary homonym of Curculio canaliculatus Olivier, 1791). Cossonus canaliculatus Fabricius, 1802 is a secondary homonym of the former and is replaced with Cossonus incisus. Salpingus fulvirostris (Fabricius, 1787) is reinstated as valid from synonymy under Salpingus planirostris (Fabricius, 1787), a primary homonym of Curculio planirostris Piller & Mitterpacher, 1783. The following new combinations are proposed: Brachysomus erinaceus (Fabricius, 1802) (from Curculio), Bronchus ferus (Gyllenhal, 1840) (from Hipporhinus), Bronchus glandifer (Fabricius, 1792) (from Curculio), Bronchus nivosus (Sparrman, 1785) (from Curculio), Bronchus sparrmani (Gyllenhal, 1833) (from Hipporhinus
Granada, Miguel A.
David Fabricius (1564-1617) was one of the most important astronomers in the period between 1596, the year of publication of Kepler's Mysterium cosmographicum, and 1609, the year of publication of the Astronomia nova.1 Kepler praised Fabricius as the most accurate observational astronomer after Tycho Brahe's death in 1601.2 Fabricius was a Reformed pastor in Ostfriesland (East Frisia), his remote natal region, and a vocational astronomer. He published nothing in the field of astronomy except for the short treatises between 1604 and 1606 concerning the nova that appeared in October 1604 in Serpentarius.
Cansi, E R; Bonorino, R; Ataíde, H S; Pujol-Luz, J R
In April 2009, a wild maned wolf, Chrysocyon brachyurus, was captured in an area of cerrado in Brasília, DF, Brazil, with screw worm maggots in external wounds. Fifty larvae were bred in the laboratory and eight adults of Cochliomyia hominivorax (Coquerel) emerged 10 days after pupation. This is the first report of a myiasis by C. hominivorax in a free-living maned wolf in Brazil.
Chen, Wenhu; Yang, Xinbo; Yang, Xiaolong; Zhai, Lei; Lu, Zekuan; Liu, Jingze; Yu, Haining
Hornets possess highly toxic venoms, which are rich in toxins, enzymes and biologically active peptides. Many bioactive substances have been identified from wasp venoms. Vespa mastoparan (MP-VBs) and Vespa chemotatic peptide presenting antimicrobial action (VESP-VBs) were purified and characterized from the venom of the wasp, Vespa bicolor Fabricius. The precursors encoding VESP-VBs and MP-VBs were cloned from the cDNA library of the venomous glands. Analyzed by FAB-MS, the amino acid sequence and molecular mass for VESP-VB1 were FMPIIGRLMSGSL and 1420.6, for MP-VB1 were INMKASAAVAKKLL and 1456.5, respectively. The primary structures of these peptides are homologous to those of chemotactic peptides and mastoparans isolated from other vespid venoms. These peptides showed strong antimicrobial activities against bacteria and fungi and induced mast cell degranulation, but displayed almost no hemolytic activity towards human blood red cells.
Thevan, Kumara; Ahmad, Abu Hassan; Rawi, Che Salmah Md; Singh, Bhupinder
In estimating the postmortem interval (PMI) using maggots obtained during autopsy, the forensic entomologist makes decisions regarding the effects of low-temperature storage of the body on the insects. In this case report, a corpse was found in an abandoned house in the residential area of Bukit Mertajam, Penang, Malaysia. The maggots were found to be alive inside the mouth of the deceased although the corpse had been in the morgue cooler for 12 days. The maggots were reared and identified as Chrysomya megacephala (Fabricius). The emerged adult flies were kept as a stock colony, and the duration of development under the indoor fluctuating temperature regime was studied. The total duration of developmental process of this species was 9.5 ± 0.5 days, and the PMI estimated was 3.2 ± 0.6 days. This case report demonstrates the survival of Ch. megacephala maggots for 12 days and their growth inside the morgue cooler.
Silva, A C; Ricalde, M P
Specimens of the mango stone weevil Sternochetus mangiferae (Fabricius) (Coleoptera: Curculionidae) were found in fruits of mango from a tree in the residential area of the Rio de Janeiro, RJ. This is the first report of the S. mangiferae in Brazil, currently regulated as an absent quarantine pest in the country. A taxonomist specialized in Curculionidae confirmed the identification based on morphological diagnostics characteristics. This detection is a relevant finding, because Brazil is a major producer and exporter of mango and the main areas of mango for exportation are located very far from this detection point. This pest damages seed and embryo of mango fruits and it causes reduction of fruit size and its premature dropping. The detection was notified to the Plant Health Department, division of the Brazilian Ministry of Agriculture, Livestock and Food Supply (MAPA), which is the National Plant Protection Organization of Brazil.
Olea, María Sofía; Centeno, Néstor; Aybar, Cecilia Adriana Veggiani; Ortega, Eugenia Silvana; Galante, Guillermina Begoña; Olea, Luis; Juri, María Julia Dantur
Myiasis is usually caused by flies of the Calliphoridae family, and Cochliomyia hominivorax is the etiological agent most frequently found in myiasis. The first case of myiasis in a diabetic foot of a 54-year-old male patient in Argentina is reported. The patient attended the hospital of the capital city of Tucumán Province for a consultation concerning an ulcer in his right foot, where the larval specimens were found. The identification of the immature larvae was based on their morphological characters, such as the cylindrical, segmented, white yellow-coloured body and tracheas with strong pigmentation. The larvae were removed, and the patient was treated with antibiotics. The larvae were reared until the adults were obtained. The adults were identified by the setose basal vein in the upper surface of the wing, denuded lower surface of the wing, short and reduced palps, and parafrontalia with black hairs outside the front row of setae. The main factor that favoured the development of myiasis is due to diabetes, which caused a loss of sensibility in the limb that resulted in late consultation. Moreover, the poor personal hygiene attracted the flies, and the foul-smelling discharge from the wound favoured the female's oviposition. There is a need to implement a program for prevention of myiasis, in which the population is made aware not only of the importance of good personal hygiene and home sanitation but also of the degree of implication of flies in the occurrence and development of this disease.
Olea, María Sofía; Centeno, Néstor; Aybar, Cecilia Adriana Veggiani; Ortega, Eugenia Silvana; Galante, Guillermina Begoña; Olea, Luis
Myiasis is usually caused by flies of the Calliphoridae family, and Cochliomyia hominivorax is the etiological agent most frequently found in myiasis. The first case of myiasis in a diabetic foot of a 54-year-old male patient in Argentina is reported. The patient attended the hospital of the capital city of Tucumán Province for a consultation concerning an ulcer in his right foot, where the larval specimens were found. The identification of the immature larvae was based on their morphological characters, such as the cylindrical, segmented, white yellow-coloured body and tracheas with strong pigmentation. The larvae were removed, and the patient was treated with antibiotics. The larvae were reared until the adults were obtained. The adults were identified by the setose basal vein in the upper surface of the wing, denuded lower surface of the wing, short and reduced palps, and parafrontalia with black hairs outside the front row of setae. The main factor that favoured the development of myiasis is due to diabetes, which caused a loss of sensibility in the limb that resulted in late consultation. Moreover, the poor personal hygiene attracted the flies, and the foul-smelling discharge from the wound favoured the female's oviposition. There is a need to implement a program for prevention of myiasis, in which the population is made aware not only of the importance of good personal hygiene and home sanitation but also of the degree of implication of flies in the occurrence and development of this disease. PMID:24623889
Maxwell, M J; Subia, J; Abrego, J; Garabed, R; Xiao, N; Toribio, R E
Larvae (maggots) of Cochliomyia hominivorax, the New World Screwworm fly, are voracious consumers of living flesh that have a negative economic impact by decreasing productivity, predisposing to other pathogens, and, in severe cases, causing death of domestic livestock. Screwworm caused extensive financial losses to the livestock industry in North America prior to its eradication. Sterile insect technique (SIT) was used to eradicate screwworm throughout North and Central America and continues to be the main tool to control it in eastern Panama. The goal of this study was to evaluate the temporal and spatial trends of screwworm myiasis cases reported in the Province of Darien and Comarca Embera (border with Colombia), Panama, from 2001 to 2011. We hypothesized that screwworm cases would vary seasonally and be spatially clustered near Colombia as a result of effective eradication strategies in Panama and the presence of an autochthonous population of flies in western Colombia. Temporal and spatial data were retrieved from COPEG-USDA records (Panama) and analysed by anova, Ripley's K function, discrete Poisson spatial statistic scan and Getis-Ord Gi*. No significant temporal trend was found, but cases were spatially distributed in four clusters. One cluster of cases occurred from 2001 to 2003 and was considered a focal temporal and spatial cluster. One cluster occurred in 2001 and 2007 indicating more rare outbreaks in an area with fewer cattle. The two remaining clusters contained cases from 2004 to 2011 and 2001 to 2011 suggesting regular breaks in the control barrier due to occasional failures of the SIT programme, difficulties implementing border quarantine strategies, livestock smuggling or the movement of infested wildlife. © 2015 Blackwell Verlag GmbH.
Pechal, Jennifer L; Moore, Hannah; Drijfhout, Falko; Benbow, M Eric
Blow flies (Diptera: Calliphoridae) are typically the first insects to arrive at human remains and carrion. Predictable succession patterns and known larval development of necrophagous insects on vertebrate remains can assist a forensic entomologist with estimates of a minimum post-mortem interval (PMImin) range. However, adult blow flies are infrequently used to estimate the PMImin, but rather are used for a confirmation of larval species identification. Cuticular hydrocarbons have demonstrated potential for estimating adult blow fly age, as hydrocarbons are present throughout blow fly development, from egg to adult, and are stable structures. The goal of this study was to identify hydrocarbon profiles associated with the adults of a North American native blow fly species, Cochliomyia macellaria (Fabricius) and a North American invasive species, Chrysomya rufifacies (Macquart). Flies were reared at a constant temperature (25°C), a photoperiod of 14:10 (L:D) (h), and were provided water, sugar and powdered milk ad libitum. Ten adult females from each species were collected at day 1, 5, 10, 20, and 30 post-emergence. Hydrocarbon compounds were extracted and then identified using gas chromatography-mass spectrometry (GC-MS) analysis. A total of 37 and 35 compounds were detected from C. macellaria and Ch. rufifacies, respectively. There were 24 and 23 n-alkene and methyl-branched alkane hydrocarbons from C. macellaria and Ch. rufifacies, respectively (10 compounds were shared between species), used for statistical analysis. Non-metric multidimensional scaling analysis and permutational multivariate analysis of variance were used to analyze the hydrocarbon profiles with significant differences (P<0.001) detected among post-emergence age cohorts for each species, and unique hydrocarbon profiles detected as each adult blow fly species aged. This work provides empirical data that serve as a foundation for future research into improving PMImin estimates made by forensic
Chicken B-cells develop in a specific organ, the bursa of Fabricius. To understand the bursal microenvironment guiding B-cell development, previous studies identified ephrin (Eph) receptor B2 (EphB2) gene transcripts in the embryonic bursa. We hypothesize that the EphB2 receptors and their ligands r...
Rossini, Michele; Vaz-de-Mello, Fernando Z.; Mann, Darren J.
Abstract After examining syntypes of Onthophagus cervicornis Kirby, 1825, previously considered to be a synonym of the North American Onthophagus striatulus (Palisot de Beauvois, 1809), we confirm the true identity and new synonymy under South Asian Onthophagus dama (Fabricius, 1798). PMID:25061364
Host responses associated with very virulent Marek’s disease virus (MDV) infection in the bursa of Fabricius of chicken was investigated. The expression of MDV pp38 antigen and MDV gB transcripts were higher at 4 days post-infection (dpi) and then showed a declining trend. On the contrary, the expre...
Batista-da-Silva, J A
Abiotic factors, such as lunar phases and tides, have a significant effect on insect development. Reproduction and immature development are usually interlinked to these abiotic factors. The tide is at its highest levels at full moon or new moon, hindering the feeding of the immature or causing their drowning. The oviposition by adult females is also compromised on these days because much of the available food is submerged. Another important abiotic factor is the wind, which displaces odoriferous particles in the air. Wind speed and direction are important elements to indicate potential sources of food for insects. I report on the effects of lunar phases, tides, and wind speed on the Calliphoridae fauna in mangrove swamps. The different species collected were identified, and the predominant species in the area were quantified. A total of 1,710 flies were collected over a 1-year period. Six Calliphoridae flies, Chloroprocta idioidea (Robineau-Desvoidy), Chrysomya megacephala (Fabricius), Chrysomya albiceps (Wiedemann), Chrysomya putoria (Wiedemann), Cochliomyia macellaria (Fabricius), and Lucilia eximia (Wiedemann) were collected. Data indicated that lunar phases have a significant effect on the abundance of C. albiceps (r = 0.39, p < 0.01), and that the variation of the tides also affected the abundance of C. putoria (r = 0.40, p < 0.00), C. macellaria (r = 0.41, p < 0.00), and C. idioidea (r = 0.31, p < 0.04). The wind speed, however, did not affect these species.
Simões, Marianna V P; Monné, Marcela L
Immatures of Mesomphalia gibbosa (Fabricius, 1781) and Mesomphalia turrita (Illiger, 1801) (Coleoptera, Chrysomelidae, Cassidinae, Mesomphaliini) are described based on specimen collections from Rio de Janeiro state, Brazil. The last-instar larva and pupal exuviae of M. gibbosa (Fabricius, 1781) and the eggs, first-instar larva, and pupa of M. turrita (Illiger, 1801) are described, photographed and illustrated, with emphasis on chaetotaxy. Additional notes on their biology are presented.
Batista-da-Silva, José A.; Moya-Borja, Gonzalo E.; Queiroz, Margareth M.C.
This study was carried out between July 2007 and June 2008 and reports on the occurrence of human myiasis caused by the New World screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) in São Gonçalo in the state of Rio de Janeiro, Brazil. Liquid or solid vaseline was used to suffocate the larvae, which were then preserved in 70% ethanol and sent to the Instituto Oswaldo Cruz for identification. C. hominivorax were identified in all 22 cases of myiasis. There were 12 male and 10 female patients with ages ranging from 03 to 71. Ethnically the highest incidence was among black people, with 17 cases. Open wounds were the main cause of the parasitosis, whereas poor personal hygiene, the low educational level, alcoholism, bedridden patients, and physical or mental disability were possibly secondary factors; in addition to all these factors the income of the patients was very low. PMID:21526934
Cammack, J A; Reiskind, M H; Guisewite, L M; Denning, S S; Watson, D W
In forensic cases involving entomological evidence, establishing the postcolonization interval (post-CI) is a critical component of the investigation. Traditional methods of estimating the post-CI rely on estimating the age of immature blow flies (Diptera: Calliphoridae) collected from remains. However, in cases of delayed discovery (e.g., when remains are located indoors), these insects may have completed their development and be present in the environment as adults. Adult fly collections are often ignored in cases of advanced decomposition because of a presumed little relevance to the investigation; herein we present information on how these insects can be of value. In this study we applied an age-grading technique to estimate the age of adults of Chrysomya megacephala (Fabricius), Cochliomyia macellaria (Fabricius), and Phormia regina (Meigen), based on the temperature-dependent accumulation of pteridines in the compound eyes, when reared at temperatures ranging from 5 to 35°C. Age could be estimated for all species*sex*rearing temperature combinations (mean r(2)±SE: 0.90±0.01) for all but P. regina reared at 5.4°C. These models can be used to increase the precision of post-CI estimates for remains found indoors, and the high r(2) values of 22 of the 24 regression equations indicates that this is a valid method for estimating the age of adult blow flies at temperatures ≥15°C. Copyright © 2017 Elsevier B.V. All rights reserved.
The Nearctic-Caribbean species Leptotrachelus dorsalis (Fabricius, 1801): Larval descriptions with a diagnosis of immature Ctenodactylini and natural history notes on the genus and tribe (Coleoptera: Carabidae)
Adults and larvae of Leptotrachelus dorsalis (Fabricius), live in association with grasses, the larvae in the appressed leaf axils. Both adult and larval L. dorsalis eat larvae of the Sugarcane Borer, Diatraea saccharalis (Fabricius), and perhaps other insects living in the confines of the leaf shea...
Chandramohan, G; Arivoli, S; Venkatesan, P
Predatory efficiency of Diplonychus rusticus (Fabricius) was recorded at different prey density with different salinity ranges. When the salinity level (ppt) was increased, the predation rate of the bug decreased. Fifth nymphal stage showed higher predation in the 2, 4 and 6 ppt levels of salinityin both 1 hr and 24 hr period of exposure at prey densities 50, 100, 150 and 200. At prey density 150, adult bugs killed more prey in the 2 ppt level of salinity in both lhr and 24 hr treatments.
Killian, Marcelo Pablo; Boviez, Juan David; Gambarotta, Mariana; Lombardo, Daniel Marcelo
Infectious bursal disease is a severe acute viral disease of young chickens, affecting mainly the B-lymphocytes in the bursa of Fabricius, leading to severe immunosuppression as a result of the death of lymphoid cells. In the bursa infected with infectious bursal disease virus, viral replication is associated with apoptosis of lymphoid cells, inflammatory change and atrophy. Vaccination has appeared to be a crucial factor for control, with live attenuated vaccines being the most used. However, the apoptotic effect of these vaccines on the bursa has not been tested. We determined the apoptotic effect caused by the most used vaccines in local production on the bursa of Fabricius cells and the correlation with histological changes. In this study, it was demonstrated that apoptosis levels in the vaccinated groups were higher than those observed in the non-vaccinated birds leading to the conclusion that the action of the live virus vaccine strains modifies the boundary of the bursa and shapes processes of cell death by apoptosis. In contrast to other studies, the vaccine strains used did not show the phenomenon of bursal atrophy during the experimental period.
Wu, Bangyuan; Cui, Hengmin; Peng, Xi; Fang, Jing; Cui, Wei; Liu, Xiaodong
The purpose of this 42-day study was to investigate the effects of methionine (Met) deficiency on immune function by determining the relative weight, morphological and ultrastructural changes of bursae of Fabricius, cell cycle, and apoptosis of bursa cells. One hundred and twenty one-day-old avian broilers were randomly divided into two groups and fed on a control diet (starter diet, Met 0.50%; grower diet, Met 0.40%) and Met-deficient diet (starter diet, Met 0.26%; grower diet, Met 0.28%) for six weeks. The relative weight of bursae was decreased with Met deficiency when compared to that of the control group. Lesions were also observed in the Met-deficient group. Histopathologically, the numbers of lymphocytes in the follicles were decreased. Ultrastructurally, the mitochondria of lymphocytes were swollen in the Met-deficient group. As measured by flow cytometry, bursal cells in the G0G1 phase were significantly higher (P < 0.01), and bursal cells in the S, G2M phases and proliferating index were obviously lower (P < 0.01) with Met deficiency than in the control group. Moreover, the percentage of apoptotic cells in the bursae were significantly increased in Met-deficient birds (P < 0.01). It was concluded that Met deficiency restrained the development of the bursae of Fabricius and affected the humoral immunity of the chickens. PMID:23486195
Michael D. Ulyshen; Louis G. Zachos; John O. Stireman; Thomas N. Sheehan; Ryan C. Garrick
1. Little is known about the biology or conservation status of Lucanus elaphus Fabricius in North America despite well-documented declines of a related species, Lucanus cervus (L.), in Europe. This study provides information critical to developing conservation plans for L. elaphus...
US Forest service, Northeastern Area, State & Private Forestry
Discovery: An overwintering colony of adult Red-haired Bark Beetles (Hylurgus ligniperda Fabricius) was discovered in November 2000 near Rochester, New York. These European beetles were found during an evaluation of white pine root decline in a Christmas tree plantation. Hylurgus ligniperda was intercepted 169 times at ports of entry in the United States between 1985...
The Old World aradid Brachyrhynchus membranaceus (Fabricius), belonging to the subfamily Mezirinae, is reported for the first time from the Western Hemisphere. Since 2005, eight specimens have been intercepted at United States ports-of-entry in international commerce from Costa Rica, Dominican Repub...
Batista-da-Silva, J A; Borja, G E M; Queiroz, M M C
This work describes a severe case of myiasis by Cochliomyia hominivorax (Coquerel) in a 59-year-old patient living in an urban area of São Gonçalo, Rio de Janeiro. The patient had an open wound on the right shoulder parasitized by 287 larvae. In order to remove the larvae, the wound was washed with NaCl and solid vaseline was applied onto the wound and covered with gauze and adhesive tape. After 90 min, the larvae were killed by asphyxiation and were removed using sterile forceps and NaCl. This procedure left the wound completely clean.
Dekeirsschieter, Jessica; Frederickx, Christine; Lognay, Georges; Brostaux, Yves; Verheggen, Francois J; Haubruge, Eric
Soon after death, carcasses release volatile chemicals that attract carrion insects including Silphidae. Nevertheless, it is not known which chemical cues are involved in the attractiveness of the carcass. So far, little information is available on the chemical ecology of carrion beetles, particularly concerning the subfamily of Silphinae. The biological role of selected cadaveric volatile organic compounds including dimethyldisulfide (DMDS), butan-1-ol, n-butanoic acid, indole, phenol, p-cresol, putrescine, and cadaverine on the silphine species, Thanatophilus sinuatus Fabricius, was investigated using both electrophysiological and behavioral techniques. Among the tested cadaveric compounds, butan-1-ol and DMDS elicited the strongest electroantennography (EAG) from both T. sinuatus male and female antennae. In a two-arm olfactometer, males and females were significantly attracted to DMDS for both tested doses, whereas only males were attracted to p-cresol at 100 ng. Putrescine was repellent to males at the dose of 1 μg.
Khangarot, B.S.; Ray, P.K.
The discharge of heavy metals into the natural waters has numerous obvious impacts on physical, chemical and biological parameters of aquatic ecosystem. Bioassay tests are important steps in establishing appropriate water quality criteria and standards for diverse use of ponds, lakes, streams and river waters. Therefore, the acute toxicities of various heavy metals to water flea Daphnia magna, and snail Lymnaea acuminata, and toad tadpoles Bufo mentanostictus have been reported from the authors' laboratory. Chironomid larvae might be particularly useful as indicators of water quality because they are widely distributed in freshwater systems and often from diverse communities within particular habitat. The aim of this study was to determine the acute toxicity of ten heavy metals to the midge larvae Chironomus tentans Fabricius, which forms an important link in aquatic food chain(s).
Moophayak, Kittikhun; Sanit, Sangob; Chaiwong, Tarinee; Sukontason, Kom; Kurahashi, Hiromu; Sukontason, Kabkaew L.; Vogtsberger, Roy C.; Bunchu, Nophawan
Stomorhina discolor (Fabricius), a species of blow fly that mimics wasps, is distributed worldwide, but detailed information about characteristics of its adult terminalia is incomplete. To help fill this gap in the information, the morphology of adult stages of S. discolor was investigated using light microscopy and scanning electron microscopy. Observations using the light microscope revealed unique characteristics of the male genitalia that are markedly different from other blow fly species. More morphological detail, including observation of several sensilla (e.g., sensilla trichoid and sensilla basiconica) along the male terminalia and female ovipositor, was seen under the scanning electron microscope. These details can be taxonomically valuable for identifying males and females of S. discolor and may help address matters concerning copulation in this species. PMID:28085083
Bovine blood inoculated and incubated with bacteria was tested to determine if adults of secondary screwworm, Cochliomyia macellaria (F.), would respond to the volatiles produced and oviposit on the incubated substrates. Five species of gram-negative coliform (Enterobacteriaceae) bacteria (Klebsiell...
Larval distribution and behavior of Chrysomya rufifacies (Macquart) (Diptera:Calliphoridae) relative to other species on Florida black bear(Carnivora:Ursidae) carcasses decompsing in North Central Florida.
Larval interactions of blow flies were documented daily temporally and spatially on 5 black bear carcasses from June – November, 2002. Cochliomyia macellaria or Chrysomya megacephala larvae were collected first, then Chrysomya rufifacies oviposited in multiple locations on the carcasses uninhabited...
Alves, A C F; Santos, W E; Farias, R C A P; Creão-Duarte, A J
Studies that focused on Calliphoridae associated with pig carcasses are abundant in southern and southeastern Brazil; however, there are few in northeast. Here, we present an inventory of the blowfly species associated with the stages of decomposition of pig carcasses in a caatinga area during dry and rainy seasons. The study took place at the Private Reserve for the Environmental Inheritance "Fazenda Almas," state of Paraíba, Brazil. Using a modified version of the Shannon trap, 32,909 adult specimens belonging to eight species were captured. During the dry season, Cochliomyia macellaria (Fabricius) (52.2%) and Chrysomya albiceps (Wiedemann) (39.9%) were the most abundant species. In the rainy season, when the majority of individuals were captured (93.7%), Chloroprocta idioidea (Robineau-Desvoidy) (71.1%) was the most abundant. Five decomposition stages were recognized, being the active decay the most attractive to colonization by blowflies, except for Lucilia eximia (Wiedemann), which was more abundant in the bloated stage.
Hajiqanbar, Hamidreza; Khaustov, Alexander; Kamali, Karim
A new species of the genus Parapygmephorus Cross, 1965 (Acari: Heterostigmatina, Neopygmephoridae) is described from Northeastern Iran. A phoretic adult female of Parapygmephorus khorasanicus Hajiqanbar and Khaustov sp. n. was discovered clasping on hairs on the ventral body surface of Halictus quadricinctus (Fabricius, 1776) (Hymenoptera; Halictidae). It is the fifth representative among the known species of the genus Parapygmephorus in the world. Differentiation of new species from other species of the genus is discussed, and a key to known world species is provided.
Before and after hatching, J-chain positive cells (JPC) were observed by immunoelectron microscopy in the chicken bursa of Fabricius. JPC were mostly lymphocytes, but epithelial cells were also detected as JPC. During the embryonic stage, J chains were mostly associated as patches with surface membranes. Furthermore, there was a diffuse localization in the cytoplasm. After hatching, J chains showed a similar subcellular localization as was seen before hatching. However, J chains were frequently detected in the cytoplasm, and rarely on the surface membranes after hatching. Staining intensities by corresponding antisera were stronger in the hatched chickens than in embryos. From these findings one may conclude that J chains are synthesized even at an early stage of B cell differentiation during embryonic life and are continuously produced at the later differentiation stages of B-cell lineage. The increased amounts of J chains estimated by staining intensity seem to coincide with B cell maturation and may correlate with signalling of IgM synthesis.
Leptocorisa oratorius (Fabricius) (Hemiptera: Alydidae) is a major rice pest which feeds on the sap of stems and rice seeds. Some graminaceous weed species serve as an alternative host of L. oratorius causing outbreaks throughout the rice growing season. Population changes of L. oratorius during both rice growing seasons - wet-season rice and dry-season rice - including the influence of alternative host, barnyard grass Echinochloa crus-galli (Graminaceae) on the development of L. oratorius was studied. Results presented that L. oratorius was the dominant pest species during the late phase of rice growth. Adults of L. oratorius started their migrations to wet-season rice at the vegetative stage of rice growth, while they migrated to dry-season rice at the repropuctive stage of rice growth. Leptocorisa oratorius breds rapidly in rice fields. Meanwhile, other adults migrated to the rice field. The population of adults and nymphs significantly increased from the reproductive stage to grain formation and ripening stage in both rice growing seasons. The population of nymphs was greater than adults but not significantly different in their number of individuals. Leptocorisa oratorius had one generation in each rice growing season. The results of the host plant study indicated that L oratorius developed completely in barnyard grass E. crus-galli as well as rice Oriza sativa (Graminaceae). However, L. oratorius preferred rice to barnyard grass for feeding and oviposition.
Perng, Fang-Shiow; Yang, Jui-Sen
The effect of ionizing radiation (2, 5, and 10 KGy of gamma rays) on muscle ultrastructure of grass shrimps ( Penaeus monodon Fabricius) tails at ambient or frozen (-18°C) temperature was investigated by transmission electron microscopy (TEM). No significant change was found in muscle ultrastructure of shrimp meat irradiated at doses of 2 or 5 KGy and then stored at 4°C for 0 and 8 days. However, in shrimps which were irradiated at ambient temperature at a dose of 10 KGy, the margin of A-band of the myofibrils in longitudinal sections exhibited an irregular zig-zag pattern. In cross section some myosin filaments in the A-band regions were missing. A possible interpretation of these results would be that 10 KGy gamma irradiation dose at ambient temperature depolymerized myosin in the A-band from the tail of thick filament. Actin damage was also observed in some cross sections. Irradiation damage of sarcoplasmic membranes appeared in the specimens irradiated at a dose of 10KGy at ambient temperature. Shrimps frozen at -18°C and then irradiated with 10 KGy did not exhibit filament damage. Freezing apparently protected shrimp meat from irradiation injury.
Yang, Yong-Qiang; Li, Xue-Bo; Shao, Ru-Yue; Lyu, Zhou; Li, Hong-Wei; Li, Gen-Ping; Xu, Lyu-Zi; Wan, Li-Hua
The characteristic life stages of infesting blowflies (Calliphoridae) such as Chrysomya megacephala (Fabricius) are powerful evidence for estimating the death time of a corpse, but an established reference of developmental times for local blowfly species is required. We determined the developmental rates of C. megacephala from southwest China at seven constant temperatures (16-34°C). Isomegalen and isomorphen diagrams were constructed based on the larval length and time for each developmental event (first ecdysis, second ecdysis, wandering, pupariation, and eclosion), at each temperature. A thermal summation model was constructed by estimating the developmental threshold temperature D0 and the thermal summation constant K. The thermal summation model indicated that, for complete development from egg hatching to eclosion, D0 = 9.07 ± 0.54°C and K = 3991.07 ± 187.26 h °C. This reference can increase the accuracy of estimations of postmortem intervals in China by predicting the growth of C. megacephala.
Muyobela, Jackson; Nkunika, Philip Obed Yobe; Mwase, Enala Tembo
The objective of the study was to determine the acaricidal properties of Bobgunnia madagascariensis (Desv.) J.H. Kirkbr. and Wiersema (Leguminosae) against adult Amblyomma variegatum (Fabricius) ticks, using Tephrosia vogelii Hook.f. (Leguminosae) as a positive control. Plant extracts of both were prepared using methanol, acetone and chloroform as extraction solvents. Methanol leaf extracts of T. vogelii (0.014 g) and methanol fruit extracts of B. madagascariensis (0.0062 g) gave the highest mean extraction weights among the plant parts and solvents used. In free contact bioassays, only methanol extracts of the bark and leaf material of T. vogelii and methanol fruit extracts of B. madagascariensis produced 100 % mortality of A. variegatum ticks in 24 h. The acaricidal activity of methanol leaf extracts of T. vogelii persisted for up to 8 days while that of fruit extracts of B. madagascariensis persisted for only 6 days. In topical application bioassays, the toxicity of T. vogelii and B. madagascariensis extracts was found to be significantly different at 95 % confidence level, with B. madagascariensis extracts (LD50 0.030 w/v) being more toxic than T. vogelii extracts (LD50 0.555 w/v). This study has shown that plant extracts of B. madagascariensis and T. vogelii extracts have significant in vitro acaricidal activity against A. variegatum ticks and can thus be considered as alternatives for tick control. Further research is however required on persistence, safety and the required application rates.
The aim of this work was to investigate developmental changes in cell proliferation and apoptosis in normal duck bursa of Fabricius using flow cytometry and immunohistochemistry. Studies were carried out on Tianfu ducks on days 24 and 27 of embryogenesis (E24 and E27) along with days 20, 70, and 200 of postnatal development (P20, P70, and P200). Results showed that the percentage of G0/G1 bursa cells significantly increased between E24 and P200 while the percentage of cells in the S phase or G2 + M phase as well as the proliferating index obviously decreased during the same period. Proliferation cell nuclear antigen was detected in lymphocyte and interfollicular epithelium. The proliferative lymphocyte density tended to decrease from E24 to P200. Apoptotic bodies in macrophages, free apoptotic bodies, or nuclei with condensed chromatin in lymphocytes in follicles were identified by transferase-mediated dUTP nick-end labeling. Both flow cytometry and microscopic analysis reveal that the proportion of apoptotic cells and apoptotic lymphocyte density increased from E24 to P20, fell on P70, then rose again on P200. Our foundings demonstrate that cell proliferation decreases and apoptosis increases with age. These changes may account for duck bursa development and involution. PMID:24962417
Beati, Lorenza; Patel, Jaymin; Lucas-Williams, Helene; Adakal, Hassane; Kanduma, Esther G; Tembo-Mwase, Enala; Krecek, Rosina; Mertins, James W; Alfred, Jeffery T; Kelly, Susyn; Kelly, Patrick
The genetic diversity of Amblyomma variegatum (Fabricius) from four Caribbean islands and five African countries was compared by analyzing the sequences of three gene fragments, two mitochondrial (12SrDNA and D-Loop-DL), and one nuclear (intergenic transcribed spacer 2 [ITS2]). Genetic variability of the ITS2 DNA fragment consisted of only uninformative single nucleotide mutations, and therefore this gene was excluded from further analyses. Mitochondrial gene divergences among African populations and between Caribbean and African populations were very low. Nevertheless, the data suggest that A. variegatum is divided into distinct East and West African groups, the western group including all Caribbean samples. Phylogenetic analyses of the 12SrDNA and DL gene sequences showed that the West African A. variegatum clustered in a well-supported monophyletic clade, distinct from eastern paraphyletic lineages. Sequences of A. variegatum from the Caribbean were embedded in the West African clade, which supports the known West African historical origin for these ticks.
Quinn, Michael James; McKernan, Moira; Lavoie, Emma T; Ottinger, Mary Ann
Effects of androgens on the development of the bursa of Fabricius are better understood than those of estradiol, despite the known sensitivity of the bursa to estradiol early in embryogenesis. The goal of this study was to determine the effects of one-time yolk injections of estradiol at day 4 of incubation on the development of the bursa and spleen as indices of treatment effects on the immune system. Follicle size and numbers in hatchling bursas were significantly reduced at 50 and 500 microg/egg, respectively. Additionally, distorted plicae and thicker epithelial layers surrounding the plicae were observed in day-old chicks at the same treatment levels. Adult bursas from birds embryonically exposed to estrogen were significantly larger than controls, suggesting an inhibition of natural bursal regression. Although estradiol altered the development of the bursa, the spleen appeared to be unaffected. The observed effects of estradiol on the development of the bursa indicate that this lymphoid organ may be a target for developmental disruption by estrogenic endocrine disrupting chemicals, though long-term consequences of embryonic exposure on immune function remain unknown.
Shu, Yinghua; Gao, Yuanyuan; Sun, Hongxia; Zou, Zhiwen; Zhou, Qiang; Zhang, Guren
Reproductive toxicity of Zn to insects was investigated in this study. By exposing phytophagous insect Spodoptera litura Fabricius to Zn in artificial diets of larvae, we investigated the effects of Zn on reproduction at ecological and molecular levels. A significantly shorter period of laying eggs was observed in S. litura exposed to 300-750mg Zn/kg. The oviposition rate, fecundity and hatchability of female adults treated with 750mg Zn/kg were significantly lower than those of the controls (31.43%, 20.95% and 52%, respectively, compared to the control). The Zn accumulation and vitellin (Vn) content in eggs were tested by inductively coupled plasma-atomic emission spectrometry and Bradford combining Western-blot, respectively. The results showed that Zn accumulated in the eggs, which has affected the weight and Vn content of eggs with significant negative correlations. The down-regulated expression levels of vitellogenin (Vg) mRNA were detected by real-time polymerase chain reaction (RT-PCR): the relative quantity of Vg mRNA was less than half of the controls at higher than 450mg Zn/kg wet weight. These results indicated that excess Zn made expression of Vg gene down-regulated and caused poor accumulation of egg yolk, which led to a reduction in egg numbers and failure of eggs to hatch.
Ruíz-Martínez, I; Gómez, F; Pérez, J M; Poudevigne, F A
In the tropics, the botfly Dermatobia hominis and the NWS Cochliomyia hominivorax are the most important myiasis agents in cattle. It is frequently reported that furuncular lesions due to D. hominis are a predisposing cause for screwworm myiasis. Our results pointed out that only 5.2 to 7.4% of C. hominivorax gravid females oviposited in the offered furuncular lesions. Of 3242 eggs layed on botfly lesions (BFL), only 82 (2.5%) developed to second instar and died. In the flies tested, the furuncular lesions due to Dermatobia were used as food supply in 81.3% of the cases. In our opinion, the role of pH, the microflora associated with BFL, and the foruncular structure were the reasons for this lack of attraction. BFL do not serve as a predisposing factor for screwworm myiasis in the tropics.
Peng, Xi; Chen, Kejie; Chen, Jin; Fang, Jing; Cui, Hengmin; Zuo, Zhicai; Deng, Junliang; Chen, Zhengli; Geng, Yi; Lai, Weimin
Aflatoxin B1 is known as a mycotoxin that develops various health problems of animals, the effects of AFB1 on thymus and bursa of Fabricius in chickens are not clear. The objective of this study was to investigate the apoptosis of thymus and bursa of Fabricius in broilers fed with AFB1 . Two hundred Avian broilers were randomly divided into four groups of 50 each, namely control group and three AFB1 groups fed with 0.15 mg, 0.3 mg, and 0.6 mg AFB1 /kg diet, respectively. In this study, flow cytometer and immunohistochemical approaches were used to determine the percentage of apoptotic cells and the expression of Bax, Bcl-2, and Caspase-3. The results showed that consumption of AFB1 diets results in increased percentage of apoptotic cells and increased expression of Caspase-3 in both thymus and bursa of Fabricius. The expression of Bax was increased and the expression of Bcl-2 was decreased in the thymus, but no significant changes in Bax and Bcl-2 expression were observed in the bursa of Fabricius when broilers fed with AFB1 . These findings suggest that adverse effects of AFB1 on thymus and bursa of Fabricius in broilers were confirmed by increased apoptotic cells and abnormal expression of Caspase-3. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1113-1120, 2016. © 2015 Wiley Periodicals, Inc.
Vicente, R E; Dáttilo, W; Izzo, T J
Although several studies have shown that ants can recognize chemical cues from their host plants in ant-plant systems, it is poorly demonstrated in ant gardens (AGs). In this interaction, ant species constantly interact with various epiphyte species. Therefore, it is possible to expect a convergence of chemical signals released by plants that could be acting to ensure that ants are able to recognize and defend epiphyte species frequently associated with AGs. In this study, it was hypothesized that ants recognize and differentiate among chemical stimuli released by AG epiphytes and non-AG epiphytes. We experimentally simulated leaf herbivore damage on three epiphyte species restricted to AGs and a locally abundant understory herb, Piper hispidum, in order to quantify the number of recruited Camponotus femoratus (Fabricius) defenders. When exposed to the AG epiphytes Peperomia macrostachya and Codonanthe uleana leaves, it was observed that the recruitment of C. femoratus workers was, on average, respectively 556% and 246% higher than control. However, the number of ants recruited by the AG epiphyte Markea longiflora or by the non-AG plant did not differ from paper pieces. This indicated that ants could discern between chemicals released by different plants, suggesting that ants can select better plants. These results can be explained by evolutionary process acting on both ants' capability in discerning plants' chemical compounds (innate attraction) or by ants' learning based on the epiphyte frequency in AGs (individual experience). To disentangle an innate behavior, a product of classical coevolutionary process, from an ant's learned behavior, is a complicated but important subject to understand in the evolution of ant-plant mutualisms.
Zuha, R M; Supriyani, M; Omar, B
Analysis on fly artifacts produced by forensically important blowfly, Chrysomya megacephala (Fabricius) (Diptera:Calliphoridae), revealed several unique patterns. They can be divided into fecal spots, regurgitation spots and swiping stains. The characteristics of fecal spots are round with three distinct levels of pigmentation; creamy, brownish and darkly pigmented. Matrix of the spots appears cloudy. The round spots are symmetrical and non-symmetrical, delineated by irregular and darker perimeter which only visible in fairly colored fecal spots. Diameter of these artifacts ranged from 0.5 mm to 4 mm. Vomit or regurgitation spots are determined by the presence of craters due to sucking activity of blowflies and surrounded by thickly raised and darker colored perimeter. The size of these specks ranged from 1 mm to 2 mm. Matrix of the spots displays irregular surface and reflective under auxiliary microscope light. Swiping stains due to defecation by flies consists of two distinguishable segments, the body and tail. It can be seen as a tear drop-like, sperm-like, snake-like and irregular tadpole-like stain. The direction of body and tail is inconsistent and length ranged between 4.8 mm to 9.2 mm. A finding that should be highlighted in this observation is the presence of crater on tadpole-like swiping stain which is apparent by its raised border characteristic and reflective under auxiliary microscope light. The directionality of this darkly brown stain is random. This unique mix of regurgitation and swiping stain has never been reported before. Highlighting the features of artifacts produced by flies would hopefully add our understanding in differentiating them from blood spatters produced from victims at crime scenes.
Androcioli, H G; Hoshino, A T; Pastório, M A; Cardoso, P C; de Araújo, P M; Fernandes, T A P; Menezes, A O
We present the first report on Euphoria lurida (Fabricius) (Coleoptera: Scarabaeidae) infestation on safflower (Carthamus tinctorius L.), a crop of industrial and medicinal importance. Between September and October 2013-2015 in Paraná State, we observed E. lurida adults feeding on safflower plants from the inception of flower head formation onwards, over an area of approximately 400 m(2). Losses in the productivity of infested plants were estimated between 15 and 50%. The damage was characterized by perforations in the upper portion and at the base of the developing flower heads or open flowers, resulting in withering and abortion of the reproductive structures.
Lee, Chi-Feng; Matsumura, Yoko
Abstract New records of four species (Lema lacertosa Lacordaire, 1845, Lema diversipes Pic, 1921, Lema cyanella (Linnaeus, 1758), Lema trivittata trivittata Say, 1824 and additional information on one recently recorded species (Lema solani Fabricius, 1798) are reported for Taiwan. Lema diversipes Pic, 1921 is removed from synonymy with Lema lacertosa Lacordaire, 1845; both species are redescribed. A lectotype is designated for Lema phungi Pic, 1924. The synonymies of Lema phungi Pic, 1924 and Lema jeanvoinei Pic, 1932 with Lema lacertosa Lacordaire, 1845 are supported. A revised key to the known species in Taiwan is provided. PMID:23653513
Yin, Shuang; Cui, Hengmin; Peng, Xi; Fang, Jing; Zuo, Zhicai; Deng, Junliang; Wang, Xun; Wu, Bangyuan; Guo, Hongrui
This study was conducted with objective of evaluating the toxic effects of nickel chloride (NiCl2) on development of bursa of Fabricius in broilers fed on diets supplemented with 0, 300, 600 and 900 mg/kg of NiCl2 for 42 days by using the methods of experimental pathology, flow cytometry (FCM), and quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that dietary NiCl2 in 300 mg/kg and over induced toxic suppression in the bursal development, which was characterized by decreasing lymphocytes histopathologically and relative weight, increasing G0/G1 phase (a prolonged nondividing state), reducing S phase (DNA replication) and proliferating index, and increasing percentages of apoptotic cells. Concurrently, the mRNA expression levels of bax, cytochrome c (cyt c), apoptotic peptidase activating factor 1 (Apaf-1), caspase-3, caspase-6, caspase-7 and caspase-9 were increased and the bcl-2 mRNA expression levels were decreased. The toxic suppression of bursal development finally impaired humoral immunity duo to the reduction of B lymphocyte population and B lymphocyte activity in the broiler chicken. This study provides new evidences for further studying the effect mechanism of Ni and Ni compoundson B-cell or bursa of Fabricius. PMID:26683707
Labonte, James R
Nebria brevicollis (Fabricius) is one of the most frequently encountered and widely distributed carabid beetles in Europe. Until recently, the only North American records were based on two single specimens, both from the 1930's in southeastern Canada. In 2008, this species was found at thirteen different sites in five counties in northwestern Oregon. As of the end of 2010, it has been found in thirty-four different sites in ten Oregon counties, with a north-south range of ~150 km and an east-west range of ~90 km. It was also detected in 2010 in southwestern Washington (Vancouver), just north of Portland and the Columbia River.The ecological amplitude of Nebria brevicollis in Oregon rivals that of the most eurytopic native carabid species, e.g., Pterostichus algidus LeConte and Scaphinotus marginatus (Fischer von Waldheim). It has been found in highly degraded heavy industrial sites, agricultural fields, city parks, gardens, second growth woodlands, mature conifer forests, montane rock gardens, and otherwise pristine stands of old growth noble fir, with elevations ranging from essentially sea level to 1,249 meters. Climates at these locales vary from that of the Mediterranean Willamette Valley floor, where snow rarely occurs and summers are hot and dry, to the summit of the Oregon Coast Range, where deep snow may be present from November through April and summers are cool. The carabid communities in which Nebria brevicollis has been found range from those predominantly of fellow exotic species, e.g., at heavily perturbed sites, to those where it is the only exotic species, such as at the Coast Range summit.Nebria brevicollis is clearly an invasive species in that it is not restricted to anthropogenic habitats, is rapidly expanding its North American range, and can be abundant in essentially pristine settings. What is not yet clear is whether it is or will become a damaging species. Although it is already the most abundant carabid species in some settings, based upon
LaBonte, James R.
Abstract Nebria brevicollis (Fabricius) is one of the most frequently encountered and widely distributed carabid beetles in Europe. Until recently, the only North American records were based on two single specimens, both from the 1930’s in southeastern Canada. In 2008, this species was found at thirteen different sites in five counties in northwestern Oregon. As of the end of 2010, it has been found in thirty-four different sites in ten Oregon counties, with a north-south range of ~150 km and an east-west range of ~90 km. It was also detected in 2010 in southwestern Washington (Vancouver), just north of Portland and the Columbia River. The ecological amplitude of Nebria brevicollis in Oregon rivals that of the most eurytopic native carabid species, e.g., Pterostichus algidus LeConte and Scaphinotus marginatus (Fischer von Waldheim). It has been found in highly degraded heavy industrial sites, agricultural fields, city parks, gardens, second growth woodlands, mature conifer forests, montane rock gardens, and otherwise pristine stands of old growth noble fir, with elevations ranging from essentially sea level to 1,249 meters. Climates at these locales vary from that of the Mediterranean Willamette Valley floor, where snow rarely occurs and summers are hot and dry, to the summit of the Oregon Coast Range, where deep snow may be present from November through April and summers are cool. The carabid communities in which Nebria brevicollis has been found range from those predominantly of fellow exotic species, e.g., at heavily perturbed sites, to those where it is the only exotic species, such as at the Coast Range summit. Nebria brevicollis is clearly an invasive species in that it is not restricted to anthropogenic habitats, is rapidly expanding its North American range, and can be abundant in essentially pristine settings. What is not yet clear is whether it is or will become a damaging species. Although it is already the most abundant carabid species in some
Nagarajan, K; Ambrose, D P
The reduviid predator, Rhynocoris fuscipes (Fabricius) (Heteroptera: Reduviidae) is a potential predator inhibiting diverse agroecosystems and preying upon about 50 minor as well as major insect pests. The prey approaching behaviour of R. fuscipes (Fabricius) to the hexane extracts of insect pests viz., Helicoverpa armigera Hubner, Spodoptera litura (Fabricius), Achaea janata Linnaeus, Dysdercus cingulatus Fabricius and Mylabris indica Thunberg was assessed in Y-shaped olfactometer in terms of Excess Proportion Index (EPI). Ether in hexane fraction extracts of S. litura stimulated higher rostral protruding activity (6.53 +/- 1.56 min) than that of H. armigera (4.61 +/- 1.29 min) followed by A. janata (3.17 +/- 1.11) and D. cingulatus (2.95 +/- 1.12 min). The lowest response was observed to the hexane extract of M. indica (1.30 +/- 0.63 min).The order of excitement in behavioral response of R. fuscipes to the tested body extract of five insect pests was ranked as follows: S. litura > H. armigera > A. janata > D. cingulatus > M. indica. Thus the present study clearly reveals the host preference of R. fuscipes to the taxonomically diverse insect pests.
Khoso, Pervez Ahmed; Yang, Zijiang; Liu, Chunpeng; Li, Shu
Selenium (Se) is necessary for the immune system in chicken and mediates its physiological functions through selenoproteins. Heat shock proteins (Hsps) are indispensable for maintaining normal cell function and for directing the immune response. The aim of the present study was to investigate the effects of Se deficiency on the messenger ribonucleic acid (mRNA) expression levels of selenoproteins and Hsps as well as immune functions in the chicken bursa of Fabricius. Two groups of chickens, namely the control and Se-deficient (L group) groups, were reared for 55 days. The chickens were offered a basal diet, which contained 0.15 mg Se/kg in the diet fed to the control group and 0.033 mg Se/kg in the diet fed to the L group. We performed real-time quantitative polymerase chain reaction to detect the mRNA expression levels of selenoproteins and Hsps on days 15, 25, 35, 45 and 55. Western blotting was used to determine the protein expression levels of Hsps on days 35, 45 and 55, and immune functions were assessed through an enzyme-linked immunosorbent assay on days 15, 35, and 55. The data showed that the mRNA expression levels of selenoproteins, such as Txnrd1, Txnrd2, Txnrd3, Dio1, Dio2, Dio3, GPx1, GPx2, GPx3 GPx4, Sepp1, Selo, Sel-15, Sepx1, Sels, Seli, Selu, Selh, and SPS2, were significantly lower (P < 0.05) in the L group compared with the control group. Additionally, the mRNA and protein expression levels of Hsps (Hsp27, Hsp40, Hsp60, Hsp70, and Hsp90) were also significantly higher (P < 0.05) in the L group. The expression levels of IL-2, IL-6, IL-8, IL-10, IL-17, IL-1β, IFN-α, IFN-β, and IFN-γ were significantly lower (P < 0.05) and TNF-α was significantly higher (P < 0.05) in the L group compared with the control group. Our results show that immunosuppression was accompanied by a downregulation of mRNA expression levels of selenoproteins and an upregulation of the Hsp mRNA expression levels. Thus, Se deficiency causes defects in the chicken bursa of
Hart, Elwood R; Weirauch, Christiane
Abstract The New World assassin bug genus Zelus Fabricius, 1803 (Insecta: Hemiptera: Heteroptera: Reduviidae: Harpactorinae: Harpactorini) is revised based on more than 10,000 specimens. Seventy-one species are recognized and twenty-four described as new: Zelus aithaleos sp. n., Zelus amblycephalus sp. n., Zelus antiguensis sp. n., Zelus auralanus sp. n., Zelus bahiaensis sp. n., Zelus banksi sp. n., Zelus casii sp. n., Zelus championi sp. n., Zelus cordazulus sp. n., Zelus fuliginatus sp. n., Zelus gilboventris sp. n., Zelus gracilipes sp. n., Zelus grandoculus sp. n., Zelus kartaboides sp. n., Zelus lewisi sp. n., Zelus panamensis sp. n., Zelus paracephalus sp. n., Zelus rosulentus sp. n., Zelus russulumus sp. n., Zelus spatulosus sp. n., Zelus truxali sp. n., Zelus umbraculoides sp. n., Zelus umbraculus sp. n., and Zelus xouthos sp. n. Five species, Zelus araneiformis Haviland, 1931, Zelus gradarius Bergroth, 1905, Zelus modestus (Stål, 1862), Zelus subfasciatus Stål, 1860 and Zelus vittaticeps Stål, 1866, are removed from Zelus and placed incertae sedis within Harpactorini. Nine new synonyms are recognized (senior synonym in parentheses): Zelus atripes Champion, 1898 syn. nov. (=Zelus conjungens [Stål, 1860]), Zelus dispar Fabricius, 1803 syn. nov. (=Zelus pedestris Fabricius, 1803), Zelus formosus Haviland, 1931 syn. nov. (=Zelus laticornis Herrich-Schaeffer, 1853), Zelus obscuridorsis (Stål, 1860) syn. nov. (=Zelus pedestris), Zelus pallidinervus Haviland, 1931 syn. nov. (=Zelus kartabensis Haviland, 1931), Zelus personatus Berg, 1879 syn. nov. (=Zelus versicolor Herrich-Schaeffer, 1848), Zelus trimaculatus Champion, 1898 syn. nov. (=Zelus means Fabricius, 1803), Zelus trimaculicollis (Stål, 1855) syn. nov. (=Zelus means), and Zelus tristis Haviland, 1931 syn. nov. (=Zelus laticornis). Zelus conjungens (Stål, 1860) stat. rev. Is resurrected from junior synonymy with zealous armillatus (Lepeletier & Seville, 1825). Zelus ambulans Stål, 1862 stat. rev
Zhang, Guanyang; Hart, Elwood R; Weirauch, Christiane
The New World assassin bug genus Zelus Fabricius, 1803 (Insecta: Hemiptera: Heteroptera: Reduviidae: Harpactorinae: Harpactorini) is revised based on more than 10,000 specimens. Seventy-one species are recognized and twenty-four described as new: Zelus aithaleos sp. n., Zelus amblycephalus sp. n., Zelus antiguensis sp. n., Zelus auralanus sp. n., Zelus bahiaensis sp. n., Zelus banksi sp. n., Zelus casii sp. n., Zelus championi sp. n., Zelus cordazulus sp. n., Zelus fuliginatus sp. n., Zelus gilboventris sp. n., Zelus gracilipes sp. n., Zelus grandoculus sp. n., Zelus kartaboides sp. n., Zelus lewisi sp. n., Zelus panamensis sp. n., Zelus paracephalus sp. n., Zelus rosulentus sp. n., Zelus russulumus sp. n., Zelus spatulosus sp. n., Zelus truxali sp. n., Zelus umbraculoides sp. n., Zelus umbraculus sp. n., and Zelus xouthos sp. n. Five species, Zelus araneiformis Haviland, 1931, Zelus gradarius Bergroth, 1905, Zelus modestus (Stål, 1862), Zelus subfasciatus Stål, 1860 and Zelus vittaticeps Stål, 1866, are removed from Zelus and placed incertae sedis within Harpactorini. Nine new synonyms are recognized (senior synonym in parentheses): Zelus atripes Champion, 1898 syn. nov. (=Zelus conjungens [Stål, 1860]), Zelus dispar Fabricius, 1803 syn. nov. (=Zelus pedestris Fabricius, 1803), Zelus formosus Haviland, 1931 syn. nov. (=Zelus laticornis Herrich-Schaeffer, 1853), Zelus obscuridorsis (Stål, 1860) syn. nov. (=Zelus pedestris), Zelus pallidinervus Haviland, 1931 syn. nov. (=Zelus kartabensis Haviland, 1931), Zelus personatus Berg, 1879 syn. nov. (=Zelus versicolor Herrich-Schaeffer, 1848), Zelus trimaculatus Champion, 1898 syn. nov. (=Zelus means Fabricius, 1803), Zelus trimaculicollis (Stål, 1855) syn. nov. (=Zelus means), and Zelus tristis Haviland, 1931 syn. nov. (=Zelus laticornis). Zelus conjungens (Stål, 1860) stat. rev. Is resurrected from junior synonymy with zealous armillatus (Lepeletier & Seville, 1825). Zelus ambulans Stål, 1862 stat. rev. and Zelus
Mahat, Naji A; Yin, Chu L; Jayaprakash, Paul T
This study investigated the influence of paraquat, a prevalent poison used by suicides, on initial oviposition and development of Chrysomya megacephala (Fabricius) using minced-beef substrates. Paraquat in lethal dose for human (40 mg/kg), two times the lethal dose (80 mg/kg) and five times the lethal dose (200 mg/kg) were mixed thoroughly with respective minced-beef substrates (1 kg each) that were decomposed in a shaded habitat fully protected from rain. Results of four replications of the above experiment revealed that the presence of paraquat neither delayed initial oviposition nor prolonged the developmental stages of C. megacephala. Therefore, estimation of postmortem interval (PMI) based on empirical baseline data obtained using animal models devoid of any poisons would still be appropriate for estimating PMI in paraquat-related deaths.
Ameri, Ali; Talebi, Ali Asghar; Beyarslan, Ahmet; Kamali, Karim; Rakhshani, Ehsan
A survey on the genus Bracon Fabricius, 1804 was conducted in Hormozgan province, Southern Iran, during February 2011-July 2012. In all, 19 species belonging to seven subgenera were collected and identified, of which seven species and the subgenus Asiabracon Tobias, 1957 are recorded for first time from Iran. Bracon (Orthobracon) persiangulfensis Ameri, Beyarslan & Talebi sp. n. is newly described and illustrated from the Queshm island of Persian Gulf. Morphological characters of the new species were compared with the congeneric species. The newly recorded species from Iran were as follow: B. (Asiabracon) quardrimaculatus Telenga, 1936; B. (Bracon) kozak Telenga, 1936; B. (Glabrobracon) immutator Nees; B. (Habrobracon) telengai (Mulyarskaya, 1955); B. (Habrobracon) variegator Spinola, 1808; B. (Orthobracon) epitriptus Marshall, 1885 and B. (Orthobracon) exhilarator Nees, 1834. A key is presented for identification of Bracon species collected in Hormozgan province as well as an updated checklist of all Bracon species occurring in Iran.
Ogasawara, Fusae; Yamamoto, Yu; Sato, Yasuo; Fukunari, Kazuhiro; Murata, Ken-Ichi; Yaegashi, Gakuji; Goto, Makiko; Murakami, Ryukoh
Concurrent fowlpox and candidiasis diseases occurred in a backyard chicken flock. Four deceased chickens (one Nagoya breed and three white silkie chickens) were examined for diagnosis. At necropsy, white curd-like plaques were observed in the crop. Fungal elements that stained positive for Candida albicans with immunohistochemistry were distributed throughout the tongue, choanal mucosa, esophagus, and crop. Typical fowlpox lesions, composed of proliferating epithelial cells with ballooning degeneration and viral intracytoplasmic inclusions, were observed in the conjunctiva, nasal mucosa, and skin around the cloaca. Interestingly, hyperplastic interfollicular epithelium with rare virus inclusions was observed in the bursa of Fabricius (BF). Some bursal follicles were replaced by proliferating epithelial cells. These proliferating cells immunohistochemically stained positive for cytokeratin. PCR and subsequent genetic sequencing detected the C. albicans gene in the crop, and fowlpox virus genes in the BF. These results indicate that this outbreak was a rare presentation of fowlpox in spontaneously infected chickens, with unusual pox lesions in the BF.
Mansikka, A; Sandberg, M; Lassila, O; Toivanen, P
We have applied polymerase-chain-reaction-directed immunoglobulin gene analysis to study the embryonic differentiation of chicken B cells. Immunoglobulin light chain DNA segments in the rearranged configuration were amplified from cells of the intraembryonic mesenchyme as early as day 7 of incubation. We showed by sequencing that the rearranged variable region genes in these early B-cell progenitors were not different from the germ-line V lambda 1 gene (the single functional light chain variable region gene in chickens). In the bursal B lymphocytes, on the other hand, clear gene conversion events were first observed at day 15 of embryonic development. The present data indicate that rearrangement of light chain genes in the chicken occurs independently of the bursa of Fabricius and that diversification of the variable region begins only later, when the surface immunoglobulin-positive B cells are proliferating in the bursal follicles. Images PMID:2123557
Houssaint, E; Diez, E; Hallet, M M
Monoclonal antibodies were raised against newborn chick bursa of Fabricius, and here we describe two antibodies, BEP-1 and BEP-2, which react selectively with the epithelial component of the bursa of Fabricius. In previous studies, using quail chick chimeric bursas, we have demonstrated that the epithelium of the bursal rudiment, presumably of endodermal origin, gives rise to the epithelium lining the bursal lumen, the basement membrane-associated epithelium and the network of reticular cells of the medulla, while the interfollicular connective cells are derived from the mesoderm. When tested in indirect immunofluorescence assay on bursa tissue sections or cell suspensions, BEP-1 reacts with a surface antigen present on all the epithelial cells of the bursa and could be used as a marker for this cell lineage. BEP-2 binds to an intracytoplasmic antigen that is present in about 5% of cells, representing the epithelial cells, and which is excreted in the medulla. BEP-2 also reacts with the epithelial cells of the thymic medulla and with the mucin-secreting goblet cells of the intestinal villi. A rabbit antiserum raised against human cytokeratin gives a different pattern of reactivity on bursal tissue compared to BEP-1 and BEP-2, tentatively suggesting that these two antibodies do not bind to keratin-like molecules. During ontogeny, BEP-1 reactivity appears in bursal epithelium from the early stages of bursal ontogeny (8 days). BEP-2 reactivity is detected around hatching time. BEP-1 and BEP-2 do not show any antigenic heterogeneity among the epithelial cells of the bursa. Images Figure 1 Figure 2 Figure 3 PMID:2423437
The Nearctic-Caribbean species Leptotrachelus dorsalis (Fabricius, 1801): Larval descriptions with a diagnosis of immature Ctenodactylini and natural history notes on the genus and tribe (Coleoptera, Carabidae)
Erwin, Terry L.; White, William H.
Abstract Adults and larvae of Leptotrachelus dorsalis (Fabricius), the Sugarcane Savior Beetle, live in association with grasses, the larvae in the appressed leaf axils. Both adult and larval Leptotrachelus dorsalis eat larvae of the Sugarcane Borer, Diatraea saccharalis (Fabricius), and perhaps other insects living in the confines of the leaf sheaths of that and other grass-like species. The geographic range of Leptotrachelus dorsalis extends from Kansas in the west to the Atlantic seaboard, north as far as Ontario, Canada and south to Cuba; it is an eastern species of North America and the Caribbean. Larval character attributes that are shared with a related ctenodactyline, Askalaphium depressum (Bates), provide a preliminary basis for characterization of the immatures of tribe Ctenodactylini. PMID:22679382
Li, Yang; He, Jun-Hua; Chen, Xue-Xin
Four subgenera of the genus Bracon Fabricius (Hymenoptera, Braconidae, Braconinae) are newly reported to China, i.e., Ophthalmobracon Tobias, 1957; Orientobracon Tobias, 2000; Punctobracon Papp, 1996; and Sculptobracon Tobias, 1961. Six species are recognized, including five new species, B. (Ophthalmobracon) elevatus sp. nov., B. (Orientobracon) maculaverticalis sp. nov., B. (Orientobracon) laticanaliculatus sp. nov., B. (Punctobracon) rhyacioniainus sp. nov. and B. (Sculptobracon) obsoletus sp. nov., which are described and illustrated. Keys to the newly recorded Chinese subgenera and species are provided.
Chaudhury, M F; Zhu, J J; Sagel, A; Chen, H; Skoda, S R
Gravid screwworm flies, Cochliomyia hominivorax (Coquerel), are attracted to the volatiles from waste larval rearing media to deposit eggs. Studies were conducted to identify volatile chemicals from the waste larval media and determine their effectiveness to attract gravid flies to oviposit. Volatiles were collected using solid-phase microextraction method, and five active chemicals, namely, dimethyl disulfide, dimethyl trisulfide, phenol, p-cresol, and indole, were identified using gas chromatography-mass spectrometry. In electroantennography studies, antennae ofgravid screwworm flies, Cochliomyia macellaria (F.), responded positively to each of the identified compounds. A synthetic blend of these five compounds in the ratio of 335:200:57:1:12 was prepared and tested for its effectiveness to attract both C. hominivorax and C. macellaria using laboratory bioassay methods. Significantly more gravid C. macellaria were attracted to and landed on substrates treated with 10-fold diluted blends compared with those landed on substrates treated with ethanol only (as control). Only a few young females and young and old males were attracted to the substrates treated with the synthetic blend. The C. hominivorax females laid significantly more eggs on substrates treated with waste media, 10-fold diluted blend, and 100-fold diluted blend than on substrates with undiluted blend or ethanol. Similarly, C. macellaria deposited significantly more eggs on substrates treated with waste media, 10-fold diluted blend, and 100-fold diluted blend compared with substrates with undiluted blend or ethanol. C. macellaria females deposited significantly less amount of eggs than did C. hominivorax females. These results indicate that the synthetic blend of five compounds identified may serve as an oviposition attractant for C. hominivorax as well as for C. macellaria.
Zong, Man Man; Zhou, Chuan Jie; Yu, Yuan Nan; Zheng, Yang; Feng, Xiu Li; Chen, Pu Yan; Yang, Mei
The bursa of Fabricius (BF) is the acknowledged humoral central immune organ and plays vital role on B lymphocyte development. In the study, a new tetrapeptide of GERA isolated from BF was named bursal peptide tetrapeptide (BP4). To investigate the immunomodulatory function of BP4 on antigen-specific immune responses, BALB/c mice were immunized with inactivated influence virus (AIV, H9N2 subtype) vaccine and BP4 at three experimental dosages. The results showed that BP4 enhanced the increased AIV-specific antibody and cytokine production, and modified T cell subtype population. Also, IgG1 antibodies were major subtype in this immune models, and the IgG1/IgG2a ratios from mice immunized with AIV vaccine and BP4 were increasing elevated with increased BP4 concentrations. Furthermore, it was noteworthy that chicken immunized with BP4 and AIV vaccine generated the increased HI antibody productions and cytokine IFN-γand IL-4 levels. Additionally, 5μg/mL BP4 stimulated antibody productions of hybridoma, which were inhibited by the specific inhibitors of PI3K/Akt, p38 MAPK, NF-κB and tyrosine phosphorylation signaling, respectively. These data hinted that BP4 might be highly relevant to the humoral immune responses, which provided a novel potential adjuvant candidate for vaccine immunization improvement and precaution on animal epidemic disease. Copyright© Bentham Science Publishers; For any queries, please email at email@example.com.
Zhu, Guang-Hui; Yu, Xiao-Jun; Xie, Liang-Xing; Luo, Hao; Wang, Dian; Lv, Jun-Yao; Xu, Xiao-Hu
Determination of the postmortem interval (PMI) is crucial for investigating homicide. However, there are currently only limited methods available. Especially, once the PMI exceeds the duration of pre-adult development of the flies with the adult emergence, its determination is very approximate. Herein, we report the regular changes in hydrocarbon composition during the weathering process of the puparia in the field in Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae), one of the common species of necrophagous flies. Correlation analysis showed that the relative abundance of nearly all of the branched alkanes and alkenes decreased significantly with the weathering time. Especially, for 9 of the peaks, over 88% of the variance in their abundance was explained by weathering time. Further analysis indicated that the regular changes caused mainly by the different weathering rates of various hydrocarbons. Additionally, the weathering rates were found to depend on the chemical structure and molecular weight of the hydrocarbons. These results indicate strongly that hydrocarbon analysis is a powerful tool for determining the weathering time of the necrophagous fly puparia, and is expected to markedly improve the determination of the late PMI. PMID:24039855
Chigray, Ivan A; Abdurakhmanov, Gayirbeg M; Nabozhenko, Maxim; Shapovalov, Andrey M
A new species of darkling beetles Blaps caspica sp. n. from Western Kazakhstan Kulaly Island (Tyuleniy Archipelago, Caspian Sea) is described. This new taxon belongs to the 6th group of the 2nd section according to Seidlitz's classification and is most similar to Blaps kadyrbekovi G. Medvedev, 2004, B. lethifera Marsham, 1802 and B. parvicollis Zoubkoff, 1829. Blaps caspica sp. n. differs from B. kadyrbekovi by the presence of a hair tuft between male abdominal ventrites 1 and 2, from B. parvicollis by having narrow acute short spurs on the mesotibiae, the structure of the gastral spicula, ovipositor and female genital tubes, from B. lethifera by the slender elliptic body, punctated (not granulated) epipleura and the structure of the female genital tubes. Images, an identification key and local distribution are given for ten Western Kazakhstan species of the genus Blaps Fabricius, 1775.
Brindis, Yolanda; Lachaud, Jean P; Gómez Y Gómez, Benigno; Rojas, Julio C; Malo, Edi A; Cruz-López, Leopoldo
Behavioral and electrophysiological tests were performed to evaluate the responses of workers of the ant Solenopsis geminata (Fabricius) from different size categories to Dufour gland extracts. Morphometric measures based in head widths across eyes were used to determine worker sizes. Trail following response of different worker sizes to Dufour gland extract from workers of different sizes was assessed. For each worker size category olfactory responses to Dufour gland extracts were determined using electroantennography (EAG). Gas chromatography and mass spectrometry (GC-MS) were used to determine the chromatographic profile of Dufour gland secretion for each worker size. Morphometric measures permitted to classify the workers of S. geminata as large, medium and small workers. Medium S. geminata workers displayed a significantly higher behavioral response to Dufour gland extracts produced by medium size workers. Similarly, medium workers showed a significantly higher EAG response to Dufour gland extracts produced by medium sized workers. Chromatographic profile of Dufour gland secretions produced by workers showed that each size category exhibited a characteristic profile of the three main components considered as potential trail pheromone constituents. This work showed that medium workers of S. geminata exhibited a high trail-following behavior as well as a high antennal response to Dufour gland secretion. This and their relative abundance in field foraging areas, suggest that medium-sized workers are specialized in foraging activities.
Zhou, Bian-hua; Liu, Li-li; Liu, Jeffrey; Yuan, Fu-wei; Tian, Er-jie; Wang, Hong-wei
The effects of diclazuril on the bursa of Fabricius (BF) structure and secretory IgA (SIgA) expression in chickens infected with Eimeria tenella were examined. The morphology of the BF was observed by hematoxylin and eosin staining, while ultrastructural changes were monitored by transmission electron microscopy. E. tenella infection caused the BF cell volumes to decrease, irregularly arranged, as well as, enlargement of the intercellular space. Diclazuril treatment alleviated the physical signs of damages associated with E. tenella infection. The SIgA expression in BF was analyzed by immunohistochemistry technique. The SIgA expression increased significantly by 350.4% (P<0.01) after E. tenella infection compared to the normal control group. With the treatment of diclazuril, the SIgA was relatively fewer in the cortex, and the expression level was significantly decreased by 46.7% (P<0.01) compared with the infected and untreated group. In conclusion, E. tenella infection in chickens induced obvious harmful changes in BF morphological structure and stimulated the expression of SIgA in the BF. Diclazuril treatment effectively alleviated the morphological changes. This result demonstrates a method to develop an immunological strategy in coccidiosis control. PMID:26797433
Zhou, Bian-Hua; Liu, Li-Li; Liu, Jeffrey; Yuan, Fu-Wei; Tian, Er-Jie; Wang, Hong-Wei
The effects of diclazuril on the bursa of Fabricius (BF) structure and secretory IgA (SIgA) expression in chickens infected with Eimeria tenella were examined. The morphology of the BF was observed by hematoxylin and eosin staining, while ultrastructural changes were monitored by transmission electron microscopy. E. tenella infection caused the BF cell volumes to decrease, irregularly arranged, as well as, enlargement of the intercellular space. Diclazuril treatment alleviated the physical signs of damages associated with E. tenella infection. The SIgA expression in BF was analyzed by immunohistochemistry technique. The SIgA expression increased significantly by 350.4% (P<0.01) after E. tenella infection compared to the normal control group. With the treatment of diclazuril, the SIgA was relatively fewer in the cortex, and the expression level was significantly decreased by 46.7% (P<0.01) compared with the infected and untreated group. In conclusion, E. tenella infection in chickens induced obvious harmful changes in BF morphological structure and stimulated the expression of SIgA in the BF. Diclazuril treatment effectively alleviated the morphological changes. This result demonstrates a method to develop an immunological strategy in coccidiosis control.
Yemshanov, Denys; Koch, Frank H; McKenney, Daniel W; Downing, Marla C; Sapio, Frank
Nonindigenous species have caused significant impacts to North American forests despite past and present international phytosanitary efforts. Though broadly acknowledged, the risks of pest invasions are difficult to quantify as they involve interactions between many factors that operate across a range of spatial and temporal scales: the transmission of invading organisms via various pathways, their spread and establishment in new environments. Our study presents a stochastic simulation approach to quantify these risks and associated uncertainties through time in a unified fashion. We outline this approach with an example of a forest pest recently detected in North America, Sirex noctilio Fabricius. We simulate new potential entries of S. noctilio as a stochastic process, based on recent volumes of marine shipments of commodities from countries where S. noctilio is established, as well as the broad dynamics of foreign marine imports. The results are then linked with a spatial model that simulates the spread of S. noctilio within the geographical distribution of its hosts (pines) while incorporating existing knowledge about its behavior in North American landscapes. Through replications, this approach yields a spatial representation of S. noctilio risks and uncertainties in a single integrated product. The approach should also be appealing to decisionmakers, since it accounts for projected flows of commodities that may serve as conduits for pest entry. Our 30-year forecasts indicate high establishment probability in Ontario, Quebec, and the northeastern United States, but further southward expansion of S. noctilio is uncertain, ultimately depending on the impact of recent international treatment standards for wood packing materials.
Doke, Dnyaneshwar; Morey, Rashmi; Dahanukar, Neelesh; Padhye, Sameer M; Paripatyadar, Shruti V
Despite being one of the dominant groups in freshwater ecosystems, morphological and ontogenetic studies on aquatic Hemiptera have received little attention in the Oriental region. We present the ontogenetic trajectory and allometry of the widespread Oriental belostomatid species, Diplonychus rusticus (Fabricius) for the first time. We have measured nine different morphological variables throughout the growth of the bug using both field captured and laboratory reared specimens. Our results suggest that the developmental instars can be distinguished by the size variables, as seen in the Principal Component Analysis. On the basis of a CHAID (Chi-squared Automatic Interaction Detection) based regression tree, we also show that the characters - total length without head and maximum width - prove to be adequate for effective instar identification. The multivariate allometric growth pattern shows that different body parts exhibit different types of allometry. This is apparent in the allometry exhibited by forelegs and mid and hind legs, which show allometry of opposite polarities. This may be due to the different functions attributed to these body parts. Our results show that the growth pattern in D. rusticus is comparable with the New World genus Belostoma, suggesting a conserved growth pattern in the family Belostomatidae. Copyright © 2017 Elsevier Ltd. All rights reserved.
Faris, A M; Wang, H-H; Tarone, A M; Grant, W E
Estimates of insect age can be informative in death investigations and, when certain assumptions are met, can be useful for estimating the postmortem interval (PMI). Currently, the accuracy and precision of PMI estimates is unknown, as error can arise from sources of variation such as measurement error, environmental variation, or genetic variation. Ecological models are an abstract, mathematical representation of an ecological system that can make predictions about the dynamics of the real system. To quantify the variation associated with the pre-appearance interval (PAI), we developed an ecological model that simulates the colonization of vertebrate remains by Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), a primary colonizer in the southern United States. The model is based on a development data set derived from a local population and represents the uncertainty in local temperature variability to address PMI estimates at local sites. After a PMI estimate is calculated for each individual, the model calculates the maximum, minimum, and mean PMI, as well as the range and standard deviation for stadia collected. The model framework presented here is one manner by which errors in PMI estimates can be addressed in court when no empirical data are available for the parameter of interest. We show that PAI is a potential important source of error and that an ecological model is one way to evaluate its impact. Such models can be re-parameterized with any development data set, PAI function, temperature regime, assumption of interest, etc., to estimate PMI and quantify uncertainty that arises from specific prediction systems. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: firstname.lastname@example.org.
George, Kelly A; Archer, Melanie S; Green, Lauren M; Conlan, Xavier A; Toop, Tes
Insect specimens collected from decomposing bodies enable forensic entomologists to estimate the minimum post-mortem interval (PMI). Drugs and toxins within a corpse may affect the development rate of insects that feed on them and it is vital to quantify these effects to accurately calculate minimum PMI. This study investigated the effects of morphine on growth rates of the native Australian blowfly, Calliphora stygia (Fabricius) (Diptera: Calliphoridae). Several morphine concentrations were incorporated into pet mince to simulate post-mortem concentrations in morphine, codeine and/or heroin-dosed corpses. There were four treatments for feeding larvae; T 1: control (no morphine); T 2: 2 microg/g morphine; T 3: 10 microg/g morphine; and T 4: 20 microg/g morphine. Ten replicates of 50 larvae were grown at 22 degrees C for each treatment and their development was compared at four comparison intervals; CI 1: 4-day-old larvae; CI 2: 7-day-old larvae; CI 3: pupae; and CI 4: adults. Length and width were measured for larvae and pupae, and costae and tibiae were measured for adults. Additionally, day of pupariation, day of adult eclosion, and survivorship were calculated for each replicate. The continued presence of morphine in meat was qualitatively verified using high-performance liquid chromatography with acidic potassium permanganate chemiluminescence detection. Growth rates of C. stygia fed on morphine-spiked mince did not differ significantly from those fed on control mince for any comparison interval or parameter measured. This suggests that C. stygia is a reliable model to use to accurately age a corpse containing morphine at any of the concentrations investigated.
Sun, Hongxia; Wu, Wenjing; Guo, Jixing; Xiao, Rong; Jiang, Fengze; Zheng, Lingyan; Zhang, Guren
Nickel is an environmental pollutant that adversely affects the male reproductive system. In the present study, the effects of nickel exposure on Spodoptera litura Fabricius were investigated by feeding larvae artificial diets containing different doses of nickel for three generations. Damage to testes and effects on male reproduction were examined. The amount of nickel that accumulated in the testes of newly emerged males increased as the nickel dose in the diet increased during a single generation. Nickel exposure increased the amount of thiobarbituric acid reactive substances and decreased the amount of glutathione in treatment groups compared with the control. The activity levels of the antioxidant response indices superoxide dismutases, catalase, and glutathione peroxidase in the testes showed variable dose-dependent relationships with nickel doses and duration of exposure. Nickel doses also disrupted the development of the testes by decreasing the weight and volume of testes and the number of eupyrene and apyrene sperm bundles in treatment groups compared with the control. When the nickel-treated males mated with normal females, fecundity was inhibited by the higher nickel doses in all three generations, but fecundity significantly increased during the second generation, which received 5 mg kg(-1) nickel. Hatching rates in all treatments significantly decreased in a dose-dependent manner in the three successive generations. The effects of nickel on these parameters correlated with the duration of nickel exposure. Results indicate assays of testes may be a novel and efficient means of evaluating the effects of heavy metals on phytophagous insects in an agricultural environment.
Wee, S L; Abdul Munir, M Z; Hee, A K W
The Artocarpus fruit fly, Bactrocera umbrosa (Fabricius) (Diptera: Tephritidae), is an oligophagous fruit pest infesting Moraceae fruits, including jackfruit (Artocarpus heterophyllus Lamarck), a fruit commodity of high value in Malaysia. The scarcity of fundamental biological, physiological and ecological information on this pest, particularly in relation to behavioural response to phytochemical lures, which are instrumental to the success of many area-wide fruit fly control and management programmes, underpins the need for studies on this much-underrated pest. The positive response of B. umbrosa males to methyl eugenol (ME), a highly potent phytochemical lure, which attracts mainly males of many Bactrocera species, was shown to increase with increasing age. As early as 7 days after emergence (DAE), ca. 22% of males had responded to ME and over 50% by 10 DAE, despite no occurrence of matings (i.e. the males were still sexually immature). Male attraction to ME peaked from 10 to 27 DAE, which corresponded with the flies' attainment of sexual maturity. In wind-tunnel assays during the dusk courtship period, ME-fed males exhibited earlier calling activity and attracted a significantly higher percentage of virgin females compared with ME-deprived males. ME-fed males enjoyed a higher mating success than ME-deprived males at 1-day post ME feeding in semi-field assays. ME consumption also promotes aggregation behaviour in B. umbrosa males, as demonstrated in wind-tunnel and semi-field assays. We suggest that ME plays a prominent role in promoting sexual communication and enhancing mating performance of the Artocarpus fruit fly, a finding that is congruent with previous reports on the consequences of ME acquisition by other economically important Bactrocera species.
Abbate, Franco; Pfarrer, Christiane; Jones, Carolyn J P; Ciriaco, Emilia; Germanà, Giovanni; Leiser, Rudolf
The present study was carried out to analyse the vascularization of the pigeon bursa cloacalis of Fabricius and to determine whether it undergoes age-dependent changes during its functionally most important growth period after hatching of the pigeon. Morphological assessment of vascular corrosion casts, studied qualitatively and quantitatively, was applied for the first time to investigate the vascularization of the pigeon pigeon bursa of Fabricius. This also allowed us to analyse the microvasculature and morphological aspects of the vessel interrelationships as occurring in the natural state. The casts were compared with histological sections stained by haematoxylin-eosin and by binding of the lectin e-PHA (Phaseolus vulgaris, erythroagglutinin) to blood vessels. The vascular architecture of the bursa of Fabricius of the pigeon revealed that the organ is irrigated via two pathways, first through the terminal capillary system of lymphoid follicles arising from the internal pudendal artery, and secondly through arteries originating from the cloacal vasculature of the collum of the organ supplying the periluminal capillary system of the pigeon bursa of Fabricius. Both systems are drained by a venous system which is collateral to the system of the internal pudendal artery and clearly functions as a direct link between the lumen and vasculature of the cloaca or gut, respectively, and the bursa fabricii. This could allow the lymphocytes to be confronted with antigens from the contents of the gut, and their subsequent transport into the secondary lymphoid organs of the organism. Our results demonstrate that the blood vessels, as major and supplying part of the lymphoid system of the bursa Fabricii, clearly reflect three different phases of development: the evolution phase from about day 20 until day 50 post-hatching, the mature phase from days 50 to 90, and the involution phase after day 90. During the evolution phase the density of the vessel system rapidly increases
Lima, Daniel José Marcondes; Lemaitre, Rafael
A male specimen of a new species of the heterogeneous genus Pagurus Fabricius, 1775, collected in 1968 off the Caribbean coast of Venezuela, was discovered among the vast crustacean collections of the Smithsonian Institution. This new species herein described and illustrated is named P. scopaopsis, and is characterized primarily by the presence of: a brush-like setation pattern on the dactyl of the left third pereopod, dense small tubercles on the dorsal surfaces of the dactyl and fixed finger of the right chela, and a raised longitudinal ridge armed with spines on the palm and fixed finger of the left chela.
Oznurlu, Y; Celik, I; Telatar, T; Sur, E
1. The effects of experimentally induced heat-stress on the embryonic development of bursa of Fabricius and thymus of the chicken were investigated by means of histological and enzyme histochemical methods. 2. In the experiments, 250 fertile eggs of the Ross 308 broiler strain were divided into two groups. The control eggs were maintained under optimal conditions (378 degrees C and 65 +/- 2% relative humidity, RH) during the whole incubation period. Heat stressed eggs were maintained under normal conditions (378 degrees C and 65 +/- 2% RH) until the 10th d of incubation and then exposed continuously (24 h per d) to high temperature (388 degrees C and 65 +/- 2% RH). Blood and tissue samples were taken from 10 animals of each group at d 13, 15, 18 and 21 of incubation and at d 2, 4 and 7 post-hatch. Tissue samples were processed for enzyme histochemical methods in addition to routine histological techniques. 3. The results revealed that egg temperatures were higher than incubator air temperature. Long-term heat-stress (401-406 degrees C egg temperature) retarded development of thymus and bursa of Fabricius. Peripheral blood ACP-ase and ANAE-positive lymphocyte levels of heat-stressed animals were lower than in the controls. 4. These results give some morphological evidence for immunosuppression induced by high temperature exposure during the embryonic development. Temperature distribution and air circulation in incubator should be questioned in the case of lower broiler flock immunity.
Zhu, Wei; Zhang, Huan; Meng, Qian; Wang, Menglong; Zhou, Guiling; Li, Xuan; Wang, Hongtuo; Miao, Lin; Qin, Qilian; Zhang, Jihong
The common cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), is a destructive pest in Asia. Although overwintering in the field has not been reported for this species, their larvae are capable of long-term survival in fluctuating temperatures, i.e., 5°C (12h) plus 13°C (12h), if food is available. With an increase in climate change due to global warming and the widespread use of greenhouses, further understanding of their cold survival strategy is needed to predict and control their population in the future. In this study, metabolomics was performed to analyze the metabolic features of S. litura larvae exposed to two typical low temperatures: 15°C and 4°C, at which the development, locomotion and feeding activities are maintained or halted, respectively. The results showed that the strategies that regulate lipid and amino acid metabolism were similar at 15°C and 4°C. Cold exposure induced a metabolic shift of energy from carbohydrate to lipid and decreased free amino acids level. Biosynthesis likely contributed to the decrease in amino acids levels even at 4°C, a non-feeding temperature, suggesting an insufficient suppression of anabolism. This explains why food and high temperature pulses are necessary for their long-term cold survival. Glycometabolism was different between 15°C and 4°C. Carbohydrates were used rapidly at 15°C, while trehalose accumulated at 4°C. Interestingly, abundant trehalose and serine are prominent features of Spodoptera exigua larvae, an overwintering species, when compared to S. litura larvae. Exposure to 4°C also induced up-regulation of carbohydrase and protease in the guts of S. litura. Therefore, it is likely that concurrence of food supplement and fluctuating temperatures could facilitate the cold survival of S. litura larvae. We also found that exposure to 4°C could activate the mevalonate pathway in S. litura larvae, which might be related to glycometabolism at 4°C. Overall, our study describes
Liu, Xiao-Dong; Feng, Xiu-Li; Zhou, Bin; Cao, Rui-Bing; Li, Xin-Feng; Ma, Zhi-Yong; Chen, Pu-Yan
The bursa of Fabricius (BF) is the central humoral immune organ unique to birds which plays important roles in B lymphocyte differentiation. Here, a new bursal peptide (BP11) with the amino acid sequence DVAGKLPDNRT was identified and characterized from BF. It was proved that BP11 promoted CFU pre-B formation, and regulated B cell differentiation, including increase the percentage of immature and mature B cells in BM cells co-cultured with IL-7. BP11 also exerted immunomodulatory function on antigen-specific immune responses in BALB/c mice immunized with inactivated influence virus (AIV, H9N2 subtype) vaccine, including enhancing AIV-specific antibody and cytokine production. Furthermore, it was noteworthy that BP11 stimulated antibody productions and potentiates the Th1 and Th2-type immune responses in dose-dependent manner in chicken. These results suggested that BP11 might be highly relevant for the development of avian immune system.
Kramer, Valerie R.; Rawlins, John E.; Verdecia, Vanessa; Daniels, Jaret C.
The Homerus swallowtail, Papilio (Pterourus) homerus Fabricius, is listed as an endangered species and is endemic to the Caribbean island of Jamaica. The largest butterfly in the Western Hemisphere, P. homerus once inhabited seven of Jamaica’s 14 parishes and consisted of at least three populations; however, now only two stronghold populations remain, a western population in the rugged Cockpit Country and an eastern population in the Blue and John Crow Mountains. Despite numerous studies of its life history, much about the population biology, including estimates of total numbers of individuals in each population, remains unknown. In addition, a breeding program is needed to establish an experimental population, which could be used to augment wild populations and ensure the continued survival of the species. Here, we present a review of the biology of P. homerus and recommendations for a conservation plan. PMID:28698508
Glick, B.; Rosse, C.
The blood and bone marrow of New Hampshire chicks were analyzed quantitatively from the time of hatch to 8 weeks of age. Hormonal bursectomy was performed by treating embryonating eggs on the 11th day of incubation with testosterone propionate (TP) which resulted in severe hypogammaglobulinemia and complete atrophy of the bursa of Fabricius. TP-treated birds exhibited some lymphocytopenia, reduced splenic weight, and lack of plasma cells in their bone marrow. The number of cells per milligram bone marrow was comparable in normal and TP-treated birds, falling in the range reported for laboratory rodents. The chick medullary hemopoiesis is characterized by the predominance of erythroblasts throughout early development; granulocytes and lymphocytes represent much smaller cellular compartments than in rodents. In the chick granulocytes tend to decrease after hatch, whereas in rodents they tend to increase. The normal chick shows a temporary increase in marrow lymphocytes after hatch, similar to that observed in some young rodents, but on a much smaller scale. Hormonal bursectomy did not prevent the development of a lymphocyte population in the bone marrow. These cells were fewer in TP-treated birds at hatch and at 4 weeks than in normal birds, but at 8 weeks of age normal and bursectomized chicks had comparable numbers of lymphocytes in their marrow. Although some lymphocytes in avain bone marrow may depend on the bursa of Fabricius for their development, a substantial proportion of bone marrow lymphocytes in the chick are bursa independent. The cell surface phenotype and site of origin of these cells remains to be investigated. 40 references, 1 figure, 3 tables.
Advantages of using development models of the carrion beetles Thanatophilus micans (Fabricius) and T. mutilatus (Castelneau) (Coleoptera: Silphidae) for estimating minimum post mortem intervals, verified with case data.
Ridgeway, J A; Midgley, J M; Collett, I J; Villet, M H
Some beetles are as useful as blow flies for estimating the minimum post mortem interval (PMImin) or time since death. Examples include Thanatophilus micans (Fabricius) and Thanatophilus mutilatus (Castelneau), two geographically and ecologically overlapping African beetles. Molecular means of identifying these species, descriptions of their natural history, thermal summation models for the development of each species, and a case in which T. micans was recovered are presented. These beetles colonise bodies soon after death, their development spans more time than that of flies, and they may be little affected by maggot-generated heat. From an experimental perspective, they can be reared individually, which allows the identification of sick individuals and has analytical advantages relative to fly larvae that must be reared in groups. Estimating minimum post mortem intervals for both species using the case data strongly suggests that developmental models parameterised for one species should not be used to make forensic estimates for closely related species for which no specific model is available and emphasises the need for correct identifications.
Bendera, M.; Ekesi, S.; Ndung'u, M.; Srinivasan, R.; Torto, B.
Previous studies on the legume pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae), a serious pest of cowpea, Vigna unguiculata (L.) Walp. (Fabales: Fabaceae), in sub-Saharan Africa have focused on sex pheromones, but the role of the host plant on sexual behavior has not been explored. We investigated this interaction in the laboratory using behavioral assays and chemical analyses. We found that the presence of cowpea seedlings and a dichloromethane extract of the leaf increased coupling in the legume pod borer by 33 and 61 %, respectively, compared to the control, suggesting the involvement of both contact and olfactory cues. We used coupled gas chromatography-electroantennographic detection (GC/EAD) and GC-mass spectrometry (GC/MS) to identify compounds from the cowpea leaf extract, detected by M. vitrata antenna. We found that the antennae of the insect consistently detected four components, with 1-octen-3-ol identified as a common and dominant component in both the volatiles released by the intact cowpea plant and leaf extract. We therefore investigated its role in the coupling of M. vitrata. In dose-response assays, 1-octen-3-ol increased coupling in M. vitrata with increasing dose of the compound compared to the control. Our results suggest that the cowpea volatile 1-octen-3-ol contributes to M. vitrata sexual behavior.
Jordaens, Kurt; Goergen, Georg; Kirk-Spriggs, Ashley H; Vokaer, Audrey; Backeljau, Thierry; De Meyer, Marc
Recently (2013-2014), several hoverfly specimens from two localities in Benin and Cameroon (West and Central Africa) were caught from a species that we could not identify using existing identification keys for Afrotropical Syrphidae. Specific identification as Toxomerus floralis (Fabricius) was accomplished using morphology and various Neotropical identification keys. Corroboration of this identification was made by sequencing of the standard COI barcode region and a subsequent BLAST-IDS in BOLD that revealed a 100% sequence similarity with Toxomerus floralis from Suriname (South America). Species identification was further supported by sequencing parts of the nuclear 18S and 28S rRNA genes. The species is widespread in Togo, Benin, Nigeria and Cameroon, and eggs, larvae and adults are abundant at several localities. Yet, the full extent of its geographic distribution within tropical Africa remains to be determined. This is only the second known established introduction of a non-African hoverfly species in the Afrotropics. Interestingly, the larvae of the species have been reported as predators of Aphididae and Delphacidae but we found them to be pollenivorous, which is a rare feeding mode within the subfamily Syrphinae. Moreover, it is the only known Syrphinae species of which the larvae feed on pollen from two plant species from different families (Cyperaceae and Orobranchaceae). This example illustrates how DNA barcoding may allow a fast and accurate identification of introduced species.
Dias, Decivaldo S; Coelho, Milton V
ATPases, an important target of insecticides, are enzymes that hydrolyze ATP and use the energy released in that process to accomplish some type of cellular work. Pachymerus nucleorum (Fabricius) larvae possess an ATPase, that presents high Ca-ATPase activity, but no Mg-ATPase activity. In the present study, the effect of zinc and copper ions in the activity Ca-ATPase of that enzyme was tested. More than 90% of the Ca-ATPase activity was inhibited in 0.5 mM of copper ions or 0.25 mM of zinc ions. In the presence of EDTA, but not in the absence, the inhibition by zinc was reverted with the increase of calcium concentration. The inhibition by copper ions was not reverted in the presence or absence of EDTA. The Ca-ATPase was not inhibited by treatment of the ATPase fraction with copper, suggesting that the copper ion does not bind directly to the enzyme. The results suggest that zinc and copper ions form a complex with ATP and bind to the enzyme inhibiting its Ca-ATPase activity.
Pisanty, Gideon; Scheuchl, Erwin; Dorchin, Netta
More than 150 solitary bee species of the genus Andrena Fabricius are known from Israel and the West Bank, where they are distributed along a broad climatic gradient and diverse habitats and vegetation types. Extensive collecting throughout Israel in recent years has yielded eight new species and one new subspecies, adding to the rich bee fauna of the region: A. crocusella Pisanty & Scheuchl n. sp., A. danini Pisanty & Scheuchl n. sp., A. hermonella Scheuchl & Pisanty n. sp., A. israelica Scheuchl & Pisanty n. sp., A. judaea Scheuchl & Pisanty n. sp., A. menahemella Scheuchl & Pisanty n. sp., A. palaestina Pisanty & Scheuchl n. sp., A. perahia Pisanty & Scheuchl n. sp., and A. sphecodimorpha mediterranea Pisanty & Scheuchl n. ssp. The previously unknown female of A. fimbriatoides Scheuchl 2004 and male of A. wolfi Gusenleitner & Scheuchl 2000 are also described here for the first time. The discovery of males of A. wolfi lead us to reinstate A. iohannescaroli Nobile 2000 as a valid taxon. Detailed morphological description and differential diagnosis against closest relatives are provided for all species, as well as information on the distribution, phenology and flower visitation, when available. A neotype is designated for A. sphecodimorpha Hedicke, the holotype of which is considered to be lost. Additional collecting efforts in diverse habitats and seasons, incorporating diverse collecting techniques, are required in order to deepen our knowledge of the rich bee fauna in threatened habitats in the Mediterranean Basin, which constitutes one of the world's major hotspots for wild bees.
Gunawardena, N E; Bandumathie, M K
Defensive secretion produced by adult males and females ofLeptocorisa oratorius, Fabricius (Hemiptera: Coreidae) living on the host plant,Oriza sativa, was analyzed by a combined gas chromatography-mass spectroscopy technique. Both male and female secretions consisted of two major components: (E)-2-octenal andn-octyl acetate, 76% and 16% (w/w), respectively. The remaining 8% were trace compounds, some of which were identified as hexyl acetate, 3-octenal, 1-octanol, and (Z)-3-octenyl acetate. In a survey among 38 coreid defensive secretions, (E)-2-octenal andn-octyl acetate were found to occur rarely in addition to coreid-specific compounds, while their combination as primary constituents was found to be unique. Toxicity and repellency of this secretion were evaluated using two household pests,Anoplolepis longipes andSitotroga cerealella, as test insects, and lethal concentration (LC50) values of 0.24 ppm and 0.14 ppm, respectively, and repellencies of 63% and 58%, respectively, were obtained. Comparing the above values with those of a pentatomid bug,Coridius janus, evaluated under the same conditions, it was apparent that this secretion has potential as a repellent to enemies ofL. oratorius but not as a toxicant to attack them. Bioassay on the alarm activity of this secretion revealed that it elicits alarm responses, alerting and dispersing aggregated male and femaleL. oratorius: this is followed by "self-coating" activities. In addition, some unique behaviors were also noted among alarmedL. oratorius.
Bendera, M; Ekesi, S; Ndung'u, M; Srinivasan, R; Torto, B
Previous studies on the legume pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae), a serious pest of cowpea, Vigna unguiculata (L.) Walp. (Fabales: Fabaceae), in sub-Saharan Africa have focused on sex pheromones, but the role of the host plant on sexual behavior has not been explored. We investigated this interaction in the laboratory using behavioral assays and chemical analyses. We found that the presence of cowpea seedlings and a dichloromethane extract of the leaf increased coupling in the legume pod borer by 33 and 61 %, respectively, compared to the control, suggesting the involvement of both contact and olfactory cues. We used coupled gas chromatography-electroantennographic detection (GC/EAD) and GC-mass spectrometry (GC/MS) to identify compounds from the cowpea leaf extract, detected by M. vitrata antenna. We found that the antennae of the insect consistently detected four components, with 1-octen-3-ol identified as a common and dominant component in both the volatiles released by the intact cowpea plant and leaf extract. We therefore investigated its role in the coupling of M. vitrata. In dose-response assays, 1-octen-3-ol increased coupling in M. vitrata with increasing dose of the compound compared to the control. Our results suggest that the cowpea volatile 1-octen-3-ol contributes to M. vitrata sexual behavior.
Fang, Jing; Liang, Na; Zhou, Mingqiang; Huang, Cheng; Peng, Xi
Aflatoxin B1 (AFB1), a toxic metabolite produced by some fungi, exerts well-known hepatocarcinogenic and immunosuppressive effects, the latter can increase the apoptotic immune cells in vitro. However, it is largely unknown that which signaling pathways contribute to excessive apoptosis of immune cells which induced by AFB1. In this study, we investigated the roles of the mitochondria, endoplasmic reticulum (ER) and death receptor activated apoptotic pathways in the bursal of Fabricius (BF) cells in the broilers exposed to AFB1 diet. We found that (1) AFB1 diet induced morphological changes in the BF. (2) FCM and TUNEL methods showed that excessive apoptosis could be resulted from AFB1 intake. (3) AFB1-induced apoptosis of bursal cells involved mitochondrial pathway (increase of Bax, Bak, cytC, caspase-9, Apaf-1, caspase-3 and decrease of Bcl-2 and Bcl-xL) and ER pathway (increase of Grp78/Bip, Grp94 and CaM). (4) Oxidative stress was confirmed in the BF of chicken fed on AFB1 diet. Overall, this work is the first to demonstrate that the activation of mitochondria and ER apoptosis pathways can lead to excessive apoptosis in BF cells, and oxidative stress is a crucial driver during AFB1 exposure. PMID:27542244
Gupta, Ankita; Gawas, Sandesh M
In rearing of Gangara thyrsis (Fabricius) (Lepidoptera: Hesperiidae) from Karnataka and Goa, India, six species of parasitoids were observed. One new species of parasitic wasp is described and illustrated: Agiommatus thyrsisae n. sp. (Hymenoptera: Pteromalidae), a solitary parasitoid reared from the egg of G. thyrsis on the natural host plant Dypsis lutescens (H. Wendl.) Beentje & J. Dransf. Three additional species of parasitic wasps were also reared: Anastatus ramakrishnai (Mani, 1935) (Hymenoptera: Eupelmidae), a solitary hyperparasitoid of A. thyrsisae n. sp.; Sympiesis thyrsisae Gupta, Gawas & Bhambure (Hymenoptera: Eulophidae), a gregarious parasitoid reared from the caterpillar of G. thyrsis on the host plant Cocos nucifera L., and Brachymeria lasus (Walker) reared from pupa of G. thyrsis on the host plant D. lutescens. Additionally, two species of tachinid flies were also reared from the pupae of G. thyrsis: Exorista sorbillans (Wiedemann, 1830) and an innominate species close to Blepharella spp. Gangara thyrsis is a new host record for the genus Agiommatus and for A. ramakrishnai and B. lasus. The mean percent parasitism in G. thyrsis eggs was 26.58% with an incubation period of 6-7 days. Amongst the egg parasitoids, 57.14-73.08% were females and 23.08% were males. Hyperparasitism ranged from 3.85 to 42.86%. Dypsis lutescens, a member of Arecaceae, is a new host plant record for G. thyrsis.
Yuan, Shibin; Wu, Bangyuan; Yu, Zhengqiang; Fang, Jing; Liang, Na; Zhou, Mingqiang; Huang, Cheng; Peng, Xi
Aflatoxin B1 (AFB1), a toxic metabolite produced by some fungi, exerts well-known hepatocarcinogenic and immunosuppressive effects, the latter can increase the apoptotic immune cells in vitro. However, it is largely unknown that which signaling pathways contribute to excessive apoptosis of immune cells which induced by AFB1. In this study, we investigated the roles of the mitochondria, endoplasmic reticulum (ER) and death receptor activated apoptotic pathways in the bursal of Fabricius (BF) cells in the broilers exposed to AFB1 diet. We found that (1) AFB1 diet induced morphological changes in the BF. (2) FCM and TUNEL methods showed that excessive apoptosis could be resulted from AFB1 intake. (3) AFB1-induced apoptosis of bursal cells involved mitochondrial pathway (increase of Bax, Bak, cytC, caspase-9, Apaf-1, caspase-3 and decrease of Bcl-2 and Bcl-xL) and ER pathway (increase of Grp78/Bip, Grp94 and CaM). (4) Oxidative stress was confirmed in the BF of chicken fed on AFB1 diet. Overall, this work is the first to demonstrate that the activation of mitochondria and ER apoptosis pathways can lead to excessive apoptosis in BF cells, and oxidative stress is a crucial driver during AFB1 exposure.
Kunthiphun, Sineenath; Endoh, Rikiya; Takashima, Masako; Ohkuma, Moriya; Tanasupawat, Somboon; Akaracharanya, Ancharida
Ninety six yeastisolates associated with dung beetles (Heliocopris bucephalus Fabricius) were examined based on culture-dependent method. A comparison of the colony morphology and PCR fingerprints obtained by (GTG)5microsatellite-primed PCR indicated that 84 of these isolates belonged to one group. Five strains (DD1-1,DD2-33, DD4-11, DD5-15 and DD6-1) were selected as the representatives of this main group, whereeach of the five selectedstrains had been derivedfrom a different dung beetle collected innorthern Thailand. A comparison of the D1/D2 domain sequence of the large subunit rRNA gene (LSU D1/D2) and the internal transcribed spacer (ITS) sequencesrevealed that these five strains were the same and wererelated to the genus Trichosporon. Phylogenetic analysis based on the LSU D1/D2 plusITSsequences placed this group within the Trichosporonbrassicae clade, but it was clearly separated from any known species. In addition, physiological tests showed that this group had the unusual property of the inability to hydrolyze urea, which was distinctly different from the related taxon. Therefore, Trichosporon heliocopridis sp. nov. (ex-type strainDD1-1T = TISTR 5946T = JCM 30786T = CBS 14168T) is proposed as a novel yeast species. The MycoBank number is MB812098.
Centeno, N; Maldonado, M; Oliva, A
Differences in the succession of insects and other Arthropoda (invertebrate animals with jointed legs), on domestic pig carcasses placed under a roof and under the open sky have been studied in Buenos Aires Province, Argentina (latitude 34 degrees 45'S) in all the seasons of the year. Faunal associations proved different for each treatment in winter: the common bluebottle Calliphora vicina was found in both, but on the sheltered carcass Cochliomyia macellaria and the rare Phaenicia cluvia were found as well. In the fall, the difference between sheltered and unsheltered carcasses was small (six species on the former and five species on the latter); in spring and summer, the difference was negligible.
Peng, Xi; Bai, Shiping; Ding, Xuemei; Zhang, Keying
This study aimed to evaluate the comparative effects of aflatoxin-contaminated corn on the thymus and bursa of Fabricius (BF) in chickens by detecting histopathological lesions, cell cycle phase distribution and apoptosis. A total of 900 COBB500 male broilers were randomly allocated into five groups. The experiment lasted for six weeks and the five dietary treatments consisted of uncontaminated corn (control), 25% contaminated corn, 50% contaminated corn, 75% contaminated corn and 100% contaminated corn groups. The gross changes showed the decreased size of the thymus and BF, as well as the pale color of the BF in the broilers after aflatoxin contaminated diet exposure. There were more nuclear debris in the thymus and BF of birds in the 50%, 75%, and 100% contaminated corn groups, but the pathological impairments of the BF were more obvious than those of the thymus, which showed as more obvious lymphocyte depletion and the proliferation of reticulocytes and fibroblasts. At 21 days of age, the percentage of thymocytes and BF cells in the G2M phase was increased in a dose-dependent manner in the four AFB-contaminated corn groups. However, at 42 days of age, dietary AFB1 induced cell cycle perturbation at the G0G1 phase in thymocytes, but at the G2M phase in BF cells. The increased percentage of apoptotic cells in the thymus and BF were similarly observed in the AFB groups. According to these results, the severity of histopathological lesions may be correlated with the different sensitivity of the two central immune organs when exposed to AFB; different arrested cell cycle phases suggest that different mechanisms may be involved in the lesions of the thymus and BF, which need to be further researched. PMID:28098787
Zhou, Xia; Guo, Yunling; Kong, Hua; Zuo, Jiao; Huang, Qixing; Jia, Ruizong; Guo, Anping; Xu, Lin
Micraspis discolor (Fabricius) (Coleoptera: Coccinellidae) is a widely distributed coleoptera predator in southern Asia in rice ecosystem, and adult M. discolor feed on both rice pollen and soft-bodied arthropods. Bitrophic bioassay and tritrophic bioassay were conducted to evaluate the potential impact of Cry1Ac/Cry1Ab-expressing rice Huahui 1 and its non-transgenic counterpart Minghui 63 on fitness parameters of adult M. discolor. The results showed that the survival, and fecundity of this beetle’ adults were not different when they fed on Bt rice or non-Bt rice pollen or Nilaparvata lugens (Stål) reared on Bt rice or non-Bt rice. Toxicity assessment to ensure M. discolor adults were not sensitive to Cry1Ab or Cry1Ac protein independent from the pollen background, M. discolor adults were fed with an artificial diet containing Cry1Ac, Cry1Ab or both protein approximately 10 times higher concentration than in Huahui 1 rice pollen. No difference was detected for any of the life-table parameters tested between Cry protein-containing and pure diet. Artificial diet containing E-64 (N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide) was included as a positive control. In contrast, the pre-oviposition and fecundity of M. discolor were significantly adversely affected by feeding on E-64-containing diet. In both bioassays, the uptakes of Cry protein by adult M. discolor were tested by ELISA measurements. These results indicated that adults of M. discolor are not affected by Cry1Ab- or Cry1Ac-expressing rice pollen and are not sensitive to Cry protein at concentrations exceeding the levels in rice pollen in Huahui1. This suggests that M. discolor adults would not be harmed by Cry1Ac/Cry1Ab rice if Bt rice Huahui 1 were commercialized. PMID:26914608
Zhou, Xia; Guo, Yunling; Kong, Hua; Zuo, Jiao; Huang, Qixing; Jia, Ruizong; Guo, Anping; Xu, Lin
Micraspis discolor (Fabricius) (Coleoptera: Coccinellidae) is a widely distributed coleoptera predator in southern Asia in rice ecosystem, and adult M. discolor feed on both rice pollen and soft-bodied arthropods. Bitrophic bioassay and tritrophic bioassay were conducted to evaluate the potential impact of Cry1Ac/Cry1Ab-expressing rice Huahui 1 and its non-transgenic counterpart Minghui 63 on fitness parameters of adult M. discolor. The results showed that the survival, and fecundity of this beetle' adults were not different when they fed on Bt rice or non-Bt rice pollen or Nilaparvata lugens (Stål) reared on Bt rice or non-Bt rice. Toxicity assessment to ensure M. discolor adults were not sensitive to Cry1Ab or Cry1Ac protein independent from the pollen background, M. discolor adults were fed with an artificial diet containing Cry1Ac, Cry1Ab or both protein approximately 10 times higher concentration than in Huahui 1 rice pollen. No difference was detected for any of the life-table parameters tested between Cry protein-containing and pure diet. Artificial diet containing E-64 (N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide) was included as a positive control. In contrast, the pre-oviposition and fecundity of M. discolor were significantly adversely affected by feeding on E-64-containing diet. In both bioassays, the uptakes of Cry protein by adult M. discolor were tested by ELISA measurements. These results indicated that adults of M. discolor are not affected by Cry1Ab- or Cry1Ac-expressing rice pollen and are not sensitive to Cry protein at concentrations exceeding the levels in rice pollen in Huahui1. This suggests that M. discolor adults would not be harmed by Cry1Ac/Cry1Ab rice if Bt rice Huahui 1 were commercialized.
de Villiers, Marelize; Kriticos, Darren J; Veldtman, Ruan
The European wasp, Vespula germanica (Fabricius) (Hymenoptera: Vespidae), is of Palaearctic origin, being native to Europe, northern Africa and Asia, and introduced into North America, Chile, Argentina, Iceland, Ascension Island, South Africa, Australia and New Zealand. Due to its polyphagous nature and scavenging behaviour, V. germanica threatens agriculture and silviculture, and negatively affects biodiversity, while its aggressive nature and venomous sting pose a health risk to humans. In areas with warmer winters and longer summers, queens and workers can survive the winter months, leading to the build-up of large nests during the following season; thereby increasing the risk posed by this species. To prevent or prepare for such unwanted impacts it is important to know where the wasp may be able to establish, either through natural spread or through introduction as a result of human transport. Distribution data from Argentina and Australia, and seasonal phenology data from Argentina were used to determine the potential distribution of V. germanica using CLIMEX modelling. In contrast to previous models, the influence of irrigation on its distribution was also investigated. Under a natural rainfall scenario, the model showed similarities to previous models. When irrigation is applied, dry stress is alleviated, leading to larger areas modelled climatically suitable compared with previous models, which provided a better fit with the actual distribution of the species. The main areas at risk of invasion by V. germanica include western USA, Mexico, small areas in Central America and in the north-western region of South America, eastern Brazil, western Russia, north-western China, Japan, the Mediterranean coastal regions of North Africa, and parts of southern and eastern Africa.
Korte, Julia; Fröhlich, Thomas; Kohn, Marina; Kaspers, Bernd; Arnold, Georg J; Härtle, Sonja
Antibody producing B-cells are an essential component of the immune system. In contrast to human and mice where B-cells develop in the bone marrow, chicken B-cells develop in defined stages in the bursa of Fabricius, a gut associated lymphoid tissue. In order to gain a better understanding of critical biological processes like immigration of B-cell precursors into the bursa anlage, their differentiation and final emigration from the bursa we analyzed the proteome dynamics of this organ during embryonic and posthatch development. Samples were taken from four representative developmental stages (embryonic day (ED) 10, ED18, day 2, and day 28) and compared in an extensive 2D DIGE approach comprising six biological replicates per time point. Cluster analysis and PCA demonstrated high reliability and reproducibility of the obtained data set and revealed distinctive proteome profiles for the selected time points, which precisely reflect the differentiation processes. One hundred fifty three protein spots with significantly different intensities were identified by MS. We detected alterations in the abundance of several proteins assigned to retinoic acid metabolism (e.g. retinal-binding protein 5) and the actin-cytoskeleton (e.g. vinculin and gelsolin). By immunohistochemistry, desmin was identified as stromal cell protein associated with the maturation of B-cell follicles. Strongest protein expression difference (10.8-fold) was observed for chloride intracellular channel 2. This protein was thus far not associated with B-cell biology but our data suggest an important function in bursa B-cell development.
Wynde, Fiona J. H.; Port, Gordon R.
Lygus rugulipennis Poppius and Liocoris tripustulatus Fabricius (Heteroptera: Miridae) are pests of glasshouse cucumber and sweet pepper crops respectively. L. rugulipennis has a wide range of foodplants, but L. tripustulatus is specialised with very few food plants. We report behavioural assessments to investigate whether either species exhibits a preference for salad over wild hosts, and whether the role of olfaction and vision in response to cues from host plants can be distinguished. Olfactory responses to leaves were tested in choice chambers. L. rugulipennis was presented nettle (wild host) and a salad leaf of cucumber or sweet pepper, where the salad leaves had higher nitrogen content. L. tripustulatus was tested with nettle and sweet pepper of two different nitrogen contents. Female L. rugulipennis spent more time on the cucumber salad host, and chose it first most often, but males showed no preference. Neither sex discriminated between sweet pepper or nettle leaves, but males made more first contacts with sweet pepper. Neither sex of L. tripustulatus discriminated between sweet pepper and nettle leaves when the sweet pepper had higher nitrogen. When the plant species contained equivalent nitrogen both sexes spent more time on nettle. There was no difference in first choice made by either sex. When visual stimuli were available, and leaves had equivalent nitrogen, L. rugulipennis showed no preference and L. tripustulatus preferred nettle leaves. We conclude that the generalist L. rugulipennis has the ability to use remote olfactory cues for host choice whereas the specialist L. tripustulatus relies mainly on contact chemosensory and gustatory cues. PMID:23226493
Movila, Alexandru; Morozov, Alexandr; Sitnicova, Natalia
The molecular genetic variability of the 12S rRNA gene, on the basis of partial primary sequence and in silico-predicted secondary structures among Dermacentor reticulatus (Fabricius 1794) ticks, was studied in the Chernobyl Nuclear Power Plant (ChNPP) Exclusion Zone. In total, 20, 20, and 25 ethanol-preserved specimens, previously collected at 3 sites with 0.76, 1.91, and 4.5 millisievert (mSv)/hr ionizing radiation background, were examined. The primary sequence analysis generated 4 haplotypes defined by 3 polymorphic sites. The most common haplotype 1 was found in all 3 locations, representing 86.2% of all sampled individuals. Haplotype 4 (10.8%) was detected at the 1.91 and 4.50 mSv/hr sites. The unique haplotypes 2 (1.5%) and 3 (1.5%) were detected only at the 1.91 and 4.50 mSv/hr sites, respectively. The haplotype diversity, nucleotide diversity, and pairwise nucleotide differences for 2 tick populations at the 1.90 and 4.50 mSv/hr sites were 0.279, 0.00085, and 0.289, and 0.397, 0.00122, 0.413, respectively. No polymorphism was detected in ticks collected at the 0.76 mSv/hr site. The primary sequences of 12S rRNA were folded into the secondary structures and the free energy of haplotypes was calculated. The free energy at 37 C (ΔG) of the nonmutant haplotype 1 and the mutant haplotypes 2, 3, and 4 were -45.79, -44.17, -39.56, and -45.79 kcal/mol, respectively. Considering the correlation between the structural profile similarity of 12S rRNA and point mutations, haplotypes 1 and 4 have similar secondary structure profiles and have received a 0.999219 similarity score in the cluster tree. The unique haplotypes 2 and 3 have differences in the secondary structure in comparison with haplotypes 1 and 4; the similarity scores were 0.914747 and 0.169431, respectively. Further studies using more genetic markers are warranted to ascertain the genetic variability and population genetic structure within D. reticulatus tick populations in the ChNPP Exclusion Zone and to
Wang, Pan; Zhang, Shichang; Li, Daiqin; Liu, Kaiyu; Wang, Guoxiu; Wang, Xiaoping; Ai, Hui
Chemoreception is a key feature in selection of host plant by phytophagous insects, and odorant-binding proteins (OBPs) are involved in chemical communication of both insects and vertebrates. The legume pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae) is one of the key pest species of cowpea and widely distributed throughout tropical and subtropical regions, causing up to 80% of yield loss. In this study, we investigated the electrophysiological responses of female M. vitrata to floral volatiles from V. unguiculata. Seventeen electroantennogram-active compounds were identified from floral volatiles of V. unguiculata by coupled gas chromatography-electroantennography (GC-EAD) and gas chromatography-mass spectrometry (GC-MS). Then, we cloned two novel full-length GOBP genes (MvitGOBP1 and MvitGOBP2) from the antennae of M. vitrata using reverse transcription PCR. Protein sequence analysis indicated that they shared high sequence similarity with other Pyralididae insect GOBPs and had the typical six-cysteine signature. Real-time PCR analysis indicated that MvitGOBP1-2 mRNA was highly expressed in the antennae of female adult with several thousands-fold difference compare to other tissue. Next, the recombinant MvitGOBP1-2 was expressed in Escherichia coli and purified using Ni ion affinity chromatography. Fluorescence binding assays demonstrated that MvitGOBP1-2 had different binding affinities with 17 volatile odorant molecules including butanoic acid butyl ester, limonene, 4-ethylpropiophenone, 1H-indol-4-ol, butanoic acid octyl ester and 2-methyl-3-phenylpropanal. In the field trapping experiment, these six floral volatiles could effectively attract female moths and showed significant difference compared with the blank lure. These results suggested that MvitGOBPs and the seventeen floral volatiles are likely to function in the olfactory behavior response of female moths, which may have played crucial roles in the selection of oviposition sites. The six
Cheng, Guangdong; Zhao, Yulian; Li, He; Wu, Yue; Li, Xianxian; Han, Qiang; Dai, Chongshan; Li, Yanhua
Forsythiaside, a phenylethanoside product isolated from air-dried fruits of Forsythia suspensa, has been demonstrated to exhibit antioxidant, antibacterial and anti-inflammatory activities in vitro. However, its mechanism and the effects of lipopolysaccharide (LPS)-induced injury on the bursa of Fabricius (BF) of chickens are poorly understood. The present study aimed to investigate the anti-inflammatory effects of forsythiaside on LPS-induced acute inflammation. In addition, the potential molecular mechanisms of forsythiaside were analyzed in the BF, a special immune organ in chickens. Forty 15-day-old chickens were randomly divided into control, LPS and LPS plus forsythiaside (30 or 60 mg/kg) groups (n=10 for each group). In the LPS plus forsythiaside (30 or 60 mg/kg) groups, the chickens were orally administered with forsythiaside at doses of 30 and 60 mg/kg for seven days. At 21 days old, the chickens were intravenously injected with 200 μg/kg body weight LPS. Chickens in the control and LPS groups were only administered with vehicle or LPS, respectively, at day 21. At 3 h post-injection, the body temperature and nitric oxide (NO) levels were analyzed. In addition, the levels and mRNA expression of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β, and the mRNA expression of nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS), were examined in the BFs isolated from the chickens. The results revealed that forsythiaside was able to attenuate the LPS-induced inflammatory responses in the BFs of the chickens. The mechanisms by which forsythiaside exerted its anti-inflammatory effect were found to correlate with the inhibition of IL-6, IL-1β, TNF-α and COX-2 production, via the inactivation of NF-κB, indicating that the NF-κB-iNOS-NO signaling pathway may be important in this process.
Bonacci, Teresa; Mazzei, Antonio; Horák, Jakub; Brandmayr, Pietro
Abstract Cucujus tulliae sp. n. is described as a new member of genus Cucujus Fabricius, 1775 (Coleoptera, Cucujidae), which enumerates at present eleven species distributed in Eurasia and northern America. This saproxylic beetle is the first Cucujus species known only from Mediterranean and it is probably endemic to Calabria (Italy). The species was found especially in old–growth mountain forests of high conservation value (i.e. national parks) dominated by Calabrian pine (Pinus laricio calabrica). We hypothesize that Cucujus tulliae sp. n. probably evolved from isolated populations of Cucujus haematodes Erichson, 1845. The species is thus relictual and of high conservation value, corresponding at least to endangered (EN) category with respect to recent IUCN criterion. Cucujus tulliae sp. n. is here compared with two species native to Europe – Cucujus haematodes and Cucujus cinnaberinus (Scopoli, 1763) and with the Caucasian Cucujus haematodes caucasicus Motschulsky, 1845, which is confirmed as a valid subspecies. The male genitalia of this Caucasian form have been examined and illustrated for the first time. A comprehensive key to adults and larvae of European species is provided. PMID:22933850
Godoy, W A; Von Zuben, F J; Von Zuben, C J; dos Reis, S F
The sensitivity of parameters that govern the stability of population size in Chrysomya albiceps and describe its spatial dynamics was evaluated in this study. The dynamics was modeled using a density-dependent model of population growth. Our simulations show that variation in fecundity and mainly in survival has marked effect on the dynamics and indicates the possibility of transitions from one-point equilibrium to bounded oscillations. C. albiceps exhibits a two-point limit cycle, but the introduction of diffusive dispersal induces an evident qualitative shift from two-point limit cycle to a one fixed-point dynamics. Population dynamics of C. albiceps is here compared to dynamics of Cochliomyia macellaria, C. megacephala and C. putoria.
Maldonado, Marcelo A; Centeno, Néstor
To quantify the potential capability of transporting and passing infective pathogens of some blowflies (Diptera: Calliphoridae), Mihályi's danger-index was calculated for seven species. The original equation was modified to include synanthropic information to discriminate between asynanthropic, hemisynanthropic, and eusynanthropic status. Three groups were recognized, of which Phaenicia cluvia and Musca domestica proved the flies with lowest index value (D = 2.93 and 3.00 respectively); Cochliomyia macellaria, Chrysomya albiceps and Sarconesia chlorogaster presented a significantly higher index value (p<0.10; D = 4.28, 4.44 and 5.66 respectively) and C. megacephala, C. vicina and P. sericata appear to represent the heaviest potential sanitary risk with the highest index value (p<0.10; D = 15.54, 16.88 and 12.49 respectively).
Gruner, Susan V.; Slone, D.H.; Capinera, J.L.
A study to determine the relative abundance and seasonality of forensically important blow flies (Diptera: Calliphoridae) in rural north-central Florida was conducted using pig carcasses (Sus scrofa L.) as models for human bodies. Seven species of Calliphoridae were collected: Lucilia coeruleiviridis (=Phoenicia) (Macquart), Cochliomyia macellaria (F.), Chrysomya rufifaces (Macquart), Phormia regina (Meigen), Chrysomya megacephala (F.), and a few specimens of Calliphora livida Hall, and Calliphora vicina Robineau-Desvoidy. Species composition in aerial collections of adult flies, preserved larval collections, and samples of larvae reared to the adult stage were all highly correlated. Relative abundance of the species found was significantly different, with L. coeruleiviridis the most abundant species year-round. The relative abundance of the collected species varied significantly by day of decomposition and by season, with significant interactions between season and day, season and species, and day and species. L. coeruleiviridis, C. macellaria, C. rufifaces, and P. regina were found during the entire year, two C. vicina specimens and 11 C. livida specimens were collected from December to March, whereas C. megacephala was collected only from June through September. ?? 2007 Entomological Society of America.
Vasconcelos, S D; Salgado, R L; Barbosa, T M; Souza, J R B
The diversity of necrophagous Diptera is largely unknown in seasonally dry tropical forests, despite their medical, veterinary, and forensic relevance. We performed a study in the dry Caatinga forest exclusive to Brazil in order to assess the diversity and temporal pattern of Diptera species using pig carcasses as substrates. Adults were collected daily until complete skeletonization. We collected 17,142 adults from 18 families, 10 of which comprise species with known necrophagous habits. The most abundant families were Calliphoridae (47.3% of specimens), Sarcophagidae (20.8%), and Muscidae (15.5%), whereas Sarcophagidae stood out in terms of richness with 21 species. The native Cochliomyia macellaria (F.) (Diptera: Calliphoridae) and the invasive Chrysomya albiceps (Wiedmann) (Calliphoridae) were the dominant species. A total of 18 species reached the carcass during the first 48 h postdeath. The bloated and active decay stages had the highest richness and abundance of dipterans. From a forensic standpoint, C. macellaria and C. albiceps are likely to aid in establishing postmortem interval due to their early arrival and high abundance on the carcass. Despite harsh environmental conditions, the Caatinga harbors a rich assemblage of dipterans that play a key role in carrion decomposition. Their medico-veterinary importance is strengthened by the poor local sanitary conditions.
Sanford, Michelle R; Pechal, Jennifer L; Tomberlin, Jeffery K
Established procedures for collecting and preserving evidence are essential for all forensic disciplines to be accepted in court and by the forensic community at large. Entomological evidence, such as Diptera larvae, are primarily preserved in ethanol, which can evaporate over time, resulting in the dehydration of specimens. In this study, methods used for rehydrating specimens were compared. The changes in larval specimens with respect to larval length and weight for three forensically important blow fly (Diptera: Calliphoridae) species in North America were quantified. Phormia regina (Meigen), Cochliomyia macellaria (F.), and Chrysomya rufifacies (Macquart) third-instar larvae were collected from various decomposing animals and preserved with three preservation methods (80% ethanol, 70% isopropyl alcohol, and hot-water kill then 80% ethanol). Preservative solutions were allowed to evaporate. Rehydration was attempted with either of the following: 80% ethanol, commercial trisodium phosphate substitute solution, or 0.5% trisodium phosphate solution. All three methods partially restored weight and length of specimens recorded before preservation. Analysis of variance results indicated that effects of preservation, rehydration treatment, and collection animal were different in each species. The interaction between preservative method and rehydration treatment had a significant effect on both P. regina and C. macellaria larval length and weight. In addition, there was a significant interaction effect of collection animal on larval C. macellaria measurements. No significant effect was observed in C. rufifacies larval length or weight among the preservatives or treatments. These methods could be used to establish a standard operating procedure for dealing with dehydrated larval specimens in forensic investigations.
Balme, G R; Denning, S S; Cammack, J A; Watson, D W
This study was undertaken to determine if immature blow flies could complete development following burial and emerge from the soil as adults. Two species of blow flies, Cochliomyia macellaria and Protophormia terraenovae, were placed at three depths and at three different life stages, in a simulated burial to evaluate the impact of soil on ascending vertical dispersal and fly survival. In soil columns, immature stages of each species were covered with 5, 25 and 50cm of soil. Emerging adult flies of both species reached the surface from all depths at all three immature stages (2nd instar, 3rd instar and pupae). At the 50-cm depth, flies were least successful in reaching the surface when buried as pupae and most successful as late 3rd instar larvae (prepupae). Collectively, more adult flies emerged from the soil if buried as 3rd instars (79.6%) than either 2nd instars or pupae (59.6% and 59.3%, respectively (F(2,159)=14.76, P<0.0001)). Similarly, at shallow burial depths of 5 and 25cm, 75.6% and 70.4% of the adults successfully reached the surface, compared with 52.6% at the 50-cm depth (F(2,159)=15.95, P<0.0001). Second instars demonstrated ascending vertical dispersal behaviours in the soil column by pupating closer to the surface. Nearly half (46.6%) of the C. macellaria 2nd instars buried in 25cm of soil pupated nearer to the surface. Similarly, 45.4% of the P. terraenovae 2nd instars pupated nearer to the surface. When buried at 50cm, approximately 25% of 2nd instars of both species pupated nearer to the surface. When 3rd instars of C. macellaria and P. terraenovae were buried at 120cm, 40% and 4.3% of the adults, respectively, successfully reached the soil surface.
Gilmour, D. G.; Theis, G. A.; Thorbecke, G. J.
F1 hybrid chicks isogenic for the strong B histocompatibility locus and for most weak H-loci were X-irradiated on day 1 after hatching, injected intraperitoneally on day 2 with dispersed cells of bursa, spleen, or thymus from 4- or 10-wk-old F1 hybrid donors, and immediately challenged by the same route with either Brucella abortus, sheep erythrocytes, or a mixture of both together. The agglutinin titers were measured in sera obtained 1 wk later. With 4-wk-old donors, a greater primary response to Brucella abortus was obtained after transfers of cells from bursa than from spleen, while thymus was much less effective. With 10-wk-old donors, the decreasing order of response was spleen, bursa, thymus. Only splenic cells were effective in transferring a response to sheep erythrocytes, at either donor age. In tests of synergism by cell mixtures from pairs of organs, the only positive finding was a modest augmentation of titer against sheep erythrocytes by bursa + spleen as compared with spleen alone. Bursal cells from 6- or 10-wk-old donors were effective in transferring a response to sheep erythrocytes when antigen injection was delayed until 5 days after cell transfer. Splenic cells from hormonally bursectomized donors were ineffective in transferring a primary response, even when the donors had been injected with antigen 1 wk before transfer. Preimmunization of normal donors led to marked increases in the responses to Brucella abortus produced by transferred splenic or thymic cells. With bursal cells, an increased response was obtained only if the interval between preimmunization and transfer was 17 rather than 7 days. With the 17-day interval, both bursal and thymic cells could also transfer a response to sheep erythrocytes. The primary sera to Brucella abortus produced after transfers of bursal or splenic cells contained almost entirely 19S antibodies. A 7S component was found in all the secondary sera tested. PMID:4994444
Pires, E M; Nogueira, R M; Pina, D S; Manica, C L M; Faroni, L R A; Moreira, P S A
Results obtained in studies can contribute to the advancement of science and innovative methods and techniques for developing practical activities. Reporting conditions that may restrict the implementation of research is critical to ensure the optimal development of further technical studies. The objective of this study was to assess the walking stability of R. dominica on a flat and smooth surface. The study was based on the determination of mortality, morphology and walking stability of the insect outside the grain mass, on a flat and smooth surface. Mortality of adults of this Coleoptera in conditions with and without food was similar, which explains the difficulty that this insect had for accessing the food source on the flat and smooth surface. The measurements of body length (BOL), width (BOW) and height (BOH) of R. dominica were compared with those of Tribolium castaneum (Coleoptera: Tenebrionidae), which showed good ability to walk in these conditions. This study indicated that the former presents lower BOL and BOW, and greater BOH than the second, and all these variables showed differences when analyzed simultaneously by means of the construction of multivariate morphometric indices (Width × Height, Length × Height and Height × Length × Width). These morphometric variables, together with the definition of the geometry most similar to the body shape, resulted in determination of the center of gravity (CG) and static rollover threshold (SRTgeom) for both species. Rhyzopertha dominica and T. castaneum presented CGs considered high and low, respectively, and together with the values obtained for SRTgeom, may justify that R. dominica can be considered a less stable species during movement, and presents greater risk of rollover on flat and smooth surfaces.
Pereira de Sousa, José Roberto; Carvalho-Filho, Fernando da Silva; Esposito, Maria Cristina
This study aimed at surveying the local calliphorid and sarcophagid species in Maranhão State (Brazil) to determine their distribution and abundance, as well as the distribution of exotic Chrysomya species. In total, 18,128 calliphorid specimens were collected, distributed in 7 genera and 14 species. The species Hemilucilia semidiaphana (Rondani, 1850) and Paralucilia paraensis (Mello, 1969) were new state records. Chrysomya albiceps (Wiedemann, 1819) and Cochliomyia macellaria (F., 1775) were the most abundant species, and the exotic species of Chrysomya together contributed more than 50% of total blow fly abundance. The abundance distribution of the calliphorid community conformed to a log series model, characterized by a steep curve that reflects an assemblage with a high degree of dominance. For the Sarcophagidae, a total of 14,810 specimens were collected and distributed in 15 genera, 11 subgenera, and 52 species. Tricharaea (Sarcophagula) occidua (F., 1794) and Peckia (Sarcodexia) lambens (Wiedemann, 1830) were the most abundant species. The abundance distribution of the species followed a log normal model, with a gentler slope, consistent with a more uniform community. The cumulative species curve for the sarcophagids did not reach the asymptote. Forty-three sarcophagid species were new state records and 22 were new records for the Brazilian northeast, which emphasizes the need for a continued survey in this region. PMID:26078304
Dufek, M I; Oscherov, E B; Damborsky, M P; Mulieri, P R
The aim of the present study was to explore the diversity and describe the spatial distribution of Calliphoridae and Sarcophagidae along an urbanization gradient in the Iberá Wetlands. The flies were collected at 18 sampling points, three per site. In total, 3,318 flies were collected (3,077 blow flies and 241 flesh flies), distributed in 13 genera and 33 species. Calliphoridae was the most abundant, comprised 92.74% of all adult flies. Chrysomya albiceps (Wiedemann), Cochliomyia macellaria (F.), Chrysomya megacephala (F.), and Chrysomya putoria (Wiedemann) were the most abundant species, representing 82.21% of the total sample. The genus Oxysarcodexia Townsend was the most diverse with 10 species represented. The abundance of exotic species represents 62.85% of the total sample, showing a reduction toward less disturbed sites. The results of this study indicated that sites with intermediate impact had higher diversity than those where the disturbances are high or absent. Our findings provide the first assessment of Calliphoridae and Sarcophagidae diversity in the area of the Iberá Wetlands. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: email@example.com.
de Sousa, José Roberto Pereira; Carvalho-Filho, Fernando da Silva; Esposito, Maria Cristina
This study aimed at surveying the local calliphorid and sarcophagid species in Maranhão State (Brazil) to determine their distribution and abundance, as well as the distribution of exotic Chrysomya species. In total, 18,128 calliphorid specimens were collected, distributed in 7 genera and 14 species. The species Hemilucilia semidiaphana (Rondani, 1850) and Paralucilia paraensis (Mello, 1969) were new state records. Chrysomya albiceps (Wiedemann, 1819) and Cochliomyia macellaria (F., 1775) were the most abundant species, and the exotic species of Chrysomya together contributed more than 50% of total blow fly abundance. The abundance distribution of the calliphorid community conformed to a log series model, characterized by a steep curve that reflects an assemblage with a high degree of dominance. For the Sarcophagidae, a total of 14,810 specimens were collected and distributed in 15 genera, 11 subgenera, and 52 species. Tricharaea (Sarcophagula) occidua (F., 1794) and Peckia (Sarcodexia) lambens (Wiedemann, 1830) were the most abundant species. The abundance distribution of the species followed a log normal model, with a gentler slope, consistent with a more uniform community. The cumulative species curve for the sarcophagids did not reach the asymptote. Forty-three sarcophagid species were new state records and 22 were new records for the Brazilian northeast, which emphasizes the need for a continued survey in this region.
Weatherbee, Courtney R; Pechal, Jennifer L; Stamper, Trevor; Benbow, M Eric
Common forensic entomology practice has been to collect the largest Diptera larvae from a scene and use published developmental data, with temperature data from the nearest weather station, to estimate larval development time and post-colonization intervals (PCIs). To evaluate the accuracy of PCI estimates among Calliphoridae species and spatially distinct temperature sources, larval communities and ambient air temperature were collected at replicate swine carcasses (N = 6) throughout decomposition. Expected accumulated degree hours (ADH) associated with Cochliomyia macellaria and Phormia regina third instars (presence and length) were calculated using published developmental data sets. Actual ADH ranges were calculated using temperatures recorded from multiple sources at varying distances (0.90 m-7.61 km) from the study carcasses: individual temperature loggers at each carcass, a local weather station, and a regional weather station. Third instars greatly varied in length and abundance. The expected ADH range for each species successfully encompassed the average actual ADH for each temperature source, but overall under-represented the range. For both calliphorid species, weather station data were associated with more accurate PCI estimates than temperature loggers associated with each carcass. These results provide an important step towards improving entomological evidence collection and analysis techniques, and developing forensic error rates.
Oliveira, Tatiana Costa; Vasconcelos, Simao Dias
Increasing rates of unsolved homicides in Brazil prompt the need for applied entomological data to be used as a complementary tool by criminal investigators. In that context, we analyzed the occurrence of forensically important insect species (Order Diptera) on 14 cadavers taken into the Institute of Legal Medicine (ILM), in Pernambuco, Brazil, according to the conditions of the body and the pattern of colonisation by insects. Simultaneously, we surveyed the diversity of insects in the surrounding environment using bait traps. Five species were present on cadavers: Chrysomya albiceps, Chrysomya megacephala and Cochliomyia macellaria (Calliphoridae), Oxysarcodexia riograndensis and Ravinia belforti (Sarcophagidae). A total of 4689 adult insects belonging to 24 species of seven dipteran families (Calliphoridae, Sarcophagidae, Muscidae, Fanniidae, Phoridae, Anthomyiidae and Stratiomyidae) was collected at the ILM premises. C. albiceps was the most frequent species on the corpses and the most abundant in the traps. Species referred to as of forensic importance, such as Lucilia eximia, Chrysomya putoria, Oxysarcodexia modesta and Ophyra chalcogaster were collected on traps, but not on cadavers. There seems to be a limited colonisation of cadavers at the scene of the death, despite the ubiquity of necrophagous species in the area. The results contribute to differentiate between species that are involved in decomposition and those found in and around the mortuary installations of the ILM, thus providing potential clues about the locality of death and the post-mortem interval.
Kranz, William; Carroll, Clinton; Dixon, Darren A.; Goodpaster, John V.; Picard, Christine J.
Alternative methods for the identification of species of blow fly pupae have been developed over the years that consist of the analyses of chemical profiles. However, the effect of biotic and abiotic factors that could influence the predictive manner for the tests have not been evaluated. The lipids of blowfly pupae (Cochliomyia macellaria, Lucilia cuprina, Lucilia sericata, and Phormia regina) were extracted in pentane, derivatized, and analyzed by total-vaporization solid phase microextraction gas chromatography-mass spectrometry (TV-SPME GC-MS). Peak areas for 26 compounds were analyzed. Here we evaluated one biotic factor (colonization) on four species of blow flies to determine how well a model produced from lipid profiles of colonized flies predicted the species of flies of offspring of wild-caught flies and found very good species identification following 10 generations of inbreeding. When we evaluated four abiotic factors in our fly rearing protocols (temperature, humidity, pupation substrate, and diet), we found that the ability to assign the chemical profile to the correct species was greatly reduced. PMID:28398264
Weatherbee, Courtney R.; Pechal, Jennifer L.; Stamper, Trevor; Benbow, M. Eric
Common forensic entomology practice has been to collect the largest Diptera larvae from a scene and use published developmental data, with temperature data from the nearest weather station, to estimate larval development time and post-colonization intervals (PCIs). To evaluate the accuracy of PCI estimates among Calliphoridae species and spatially distinct temperature sources, larval communities and ambient air temperature were collected at replicate swine carcasses (N = 6) throughout decomposition. Expected accumulated degree hours (ADH) associated with Cochliomyia macellaria and Phormia regina third instars (presence and length) were calculated using published developmental data sets. Actual ADH ranges were calculated using temperatures recorded from multiple sources at varying distances (0.90 m–7.61 km) from the study carcasses: individual temperature loggers at each carcass, a local weather station, and a regional weather station. Third instars greatly varied in length and abundance. The expected ADH range for each species successfully encompassed the average actual ADH for each temperature source, but overall under-represented the range. For both calliphorid species, weather station data were associated with more accurate PCI estimates than temperature loggers associated with each carcass. These results provide an important step towards improving entomological evidence collection and analysis techniques, and developing forensic error rates. PMID:28375172
Kranz, William; Carroll, Clinton; Dixon, Darren A; Goodpaster, John V; Picard, Christine J
Alternative methods for the identification of species of blow fly pupae have been developed over the years that consist of the analyses of chemical profiles. However, the effect of biotic and abiotic factors that could influence the predictive manner for the tests have not been evaluated. The lipids of blowfly pupae (Cochliomyia macellaria, Lucilia cuprina, Lucilia sericata, and Phormia regina) were extracted in pentane, derivatized, and analyzed by total-vaporization solid phase microextraction gas chromatography-mass spectrometry (TV-SPME GC-MS). Peak areas for 26 compounds were analyzed. Here we evaluated one biotic factor (colonization) on four species of blow flies to determine how well a model produced from lipid profiles of colonized flies predicted the species of flies of offspring of wild-caught flies and found very good species identification following 10 generations of inbreeding. When we evaluated four abiotic factors in our fly rearing protocols (temperature, humidity, pupation substrate, and diet), we found that the ability to assign the chemical profile to the correct species was greatly reduced.
Bioassays of 6 racemic synthesized candidate sex pheromone compounds against male New World Screwworm flies showed that the most potent bioactivity was found with 6-acetoxy-19-methylnonacosane and 7-acetoxy-15-methylnonacosane compared to 4 other isomeric acetoxy nonacosanes. Since all these methyl-...
Fruean, Sn; East, Ij
To assess Australia's targeted surveillance to detect an incursion of screw-worm fly (Chrysomya bezziana). A multi-criteria analysis shell was used to combine data on potential pathways of entry, availability of host species and environmental factors affecting survival of screw-worm fly in order to map spatial variation in the relative likelihood of a screw-worm fly incursion into Australia. Australia's current screw-worm fly surveillance activities were reviewed to determine whether they are located in the areas of highest likelihood of an incursion. Under average environmental conditions, an incursion of screw-worm fly in Australia is relatively more likely to occur along the north coast, down the eastern seaboard or in the south-east. Cold winter temperatures would limit the environmental suitability for screw-worm fly survival to the north and north-east coast and adjacent inland areas. Australia's current targeted surveillance conducted by the Northern Australia Quarantine Strategy program of the Australian Department of Agriculture (adult screw-worm fly trapping and myiasis sampling) correlated well with areas considered to have a high relative likelihood of an incursion of screw-worm fly. Adult fly trapping conducted at sea ports was less well correlated. Changes to surveillance at sea ports are proposed to better target areas considered to have a higher relative likelihood of screw-worm fly incursion. These include increasing the trapping intensity along the north and north-east coasts and shifting surveillance activity from the west coast to the south-east. © 2014 Australian Veterinary Association.
Perez, Anne E; Haskell, Neal H; Wells, Jeffrey D
Carrion insect succession patterns have long been used to estimate the postmortem interval (PMI) during a death investigation. However, no published carrion succession study included sufficient replication to calculate a confidence interval about a PMI estimate based on occurrence data. We exposed 53 pig carcasses (16±2.5 kg), near the likely minimum needed for such statistical analysis, at a site in north-central Indiana, USA, over three consecutive summer seasons. Insects and Collembola were sampled daily from each carcass for a total of 14 days, by this time each was skeletonized. The criteria for judging a life stage of a given species to be potentially useful for succession-based PMI estimation were (1) nonreoccurrence (observed during a single period of presence on a corpse), and (2) found in a sufficiently large proportion of carcasses to support a PMI confidence interval. For this data set that proportion threshold is 45/53. Of the 266 species collected and identified, none was nonreoccuring in that each showed at least a gap of one day on a single carcass. If the definition of nonreoccurrence is relaxed to include such a single one-day gap the larval forms of Necrophilaamericana, Fanniascalaris, Cochliomyia macellaria, Phormiaregina, and Luciliaillustris satisfied these two criteria. Adults of Creophilus maxillosus, Necrobiaruficollis, and Necrodessurinamensis were common and showed only a few, single-day gaps in occurrence. C.maxillosus, P.regina, and L.illustris displayed exceptional forensic utility in that they were observed on every carcass. Although these observations were made at a single site during one season of the year, the species we found to be useful have large geographic ranges. We suggest that future carrion insect succession research focus only on a limited set of species with high potential forensic utility so as to reduce sample effort per carcass and thereby enable increased experimental replication.
Slone, D.H.; Gruner, Susan V.
The growth and development of carrion-feeding calliphorid (Diptera Calliphoridae) larvae, or maggots, is of great interest to forensic sciences, especially for estimation of a postmortem interval (PMI). The development rate of calliphorid larvae is influenced by the temperature of their immediate environment. Heat generation in larval feeding aggregations (=maggot masses) is a well-known phenomenon, but it has not been quantitatively described. Calculated development rates that do not include internally generated temperatures will result in overestimation of PMI. Over a period of 2.5 yr, 80 pig, Sus scrofa L., carcasses were placed out at study sites in north central Florida and northwestern Indiana. Once larval aggregations started to form, multiple internal and external temperatures, and weather observations were taken daily or every few days between 1400 and 1800 hours until pupation of the larvae. Volume of each aggregation was determined by measuring surface area and average depth. Live and preserved samples of larvae were taken for species identification. The four most common species collected were Lucilia coeruleiviridis (=Phaenicia) (Macquart) (77%), Cochliomyia macellaria (F.) (8.3%), Chrysomya rufifaces (Macquart) (7.7%), and Phormia regina (Meigen) (5.5%). Statistical analyses showed that 1) volume of a larval mass had a strong influence on its temperature, 2) internal temperatures of masses on the ground were influenced by soil temperature and mass volume, 3) internal temperatures of masses smaller than 20 cm3 were influenced by ambient air temperature and mass volume, and 4) masses larger than 20 cm3 on the carcass had strongly regulated internal temperatures determined only by the volume of the mass, with larger volumes associated with higher temperatures. Nonsignificant factors included presence of rain or clouds, shape of the aggregation, weight of the carcass, species composition of the aggregation, time since death, or season.
Slone, D H; Gruner, S V
The growth and development of carrion-feeding calliphorid (Diptera: Calliphoridae) larvae, or maggots, is of great interest to forensic sciences, especially for estimation of a postmortem interval (PMI). The development rate of calliphorid larvae is influenced by the temperature of their immediate environment. Heat generation in larval feeding aggregations (=maggot masses) is a well-known phenomenon, but it has not been quantitatively described. Calculated development rates that do not include internally generated temperatures will result in overestimation of PMI. Over a period of 2.5 yr, 80 pig, Sus scrofa L., carcasses were placed out at study sites in north central Florida and northwestern Indiana. Once larval aggregations started to form, multiple internal and external temperatures, and weather observations were taken daily or every few days between 1400 and 1800 hours until pupation of the larvae. Volume of each aggregation was determined by measuring surface area and average depth. Live and preserved samples of larvae were taken for species identification. The four most common species collected were Lucilia coeruleiviridis (=Phaenicia) (Macquart) (77%), Cochliomyia macellaria (F.) (8.3%), Chrysomya rufifaces (Macquart) (7.7%), and Phormnia regina (Meigen) (5.5%). Statistical analyses showed that 1) volume of a larval mass had a strong influence on its temperature, 2) internal temperatures of masses on the ground were influenced by soil temperature and mass volume, 3) internal temperatures of masses smaller than 20 cm3 were influenced by ambient air temperature and mass volume, and 4) masses larger than 20 cm3 on the carcass had strongly regulated internal temperatures determined only by the volume of the mass, with larger volumes associated with higher temperatures. Nonsignificant factors included presence of rain or clouds, shape of the aggregation, weight of the carcass, species composition of the aggregation, time since death, or season.
Luo, Chen; Luo, Kun; Meng, Linqin; Wan, Bin; Zhao, Huiyan; Hu, Zuqing
Many insects harbor heritable endosymbionts, whether obligatory or facultative, and the role of facultative endosymbionts in shaping the phenotype of these species has become increasingly important. However, little is known about whether micro-injected endosymbionts can have any effects on aphid clones, which was measured using various ecological parameters. We examined the effects between symbiotic treatments and the vital life history traits generated by Regiella insecticola on the life table parameters of Sitobion avenae. The results showed that R. insecticola can decrease the intrinsic rate of increase (r), the finite rate of increase (λ) and birth rate and can increase the mean generation times (T) of S. avenae clones, suggesting that R. insecticola may decelerate the normal development of the hosts. No significant differences of these parameters were observed between the examined Sitobion avenae clones, and the symbiont treatment by genotype interaction affected only the net reproduction rate R0, pre-adult duration and total longevity but not the other parameters. Additionally, a population projection showed that R. insecticola decelerated the growth of the S. avenae clones. The evocable effects of R. insecticola on the S. avenae clones may have significant ramifications for the control of S. avenae populations under field/natural conditions. PMID:28094341
Beaurepaire, Alexis L; Kraus, Bernard F; Koeniger, Gudrun; Koeniger, Nikolaus; Lim, Herbert; Moritz, Robin F A
The giant honeybee Apis dorsata often forms dense colony aggregations which can include up to 200 often closely related nests in the same location, setting the stage for inbred matings. Yet, like in all other Apis species, A. dorsata queens mate in mid-air on lek like drone congregation areas (DCAs) where large numbers of males gather in flight. We here report how the drone composition of A. dorsata DCAs facilitates outbreeding, taking into the account both spatial (three DCAs) and temporal (subsequent sampling days) dynamics. We compared the drones' genotypes at ten microsatellite DNA markers with those of the queen genotypes of six drone-producing colonies located close to the DCAs (Tenom, Sabah, Malaysia). None of 430 sampled drones originated from any of these nearby colonies. Moreover, we estimated that 141 unidentified colonies were contributing to the three DCAs. Most of these colonies were participating multiple times in the different locations and/or during the consecutive days of sampling. The drones sampled in the DCAs could be attributed to six subpopulations. These were all admixed in all DCA samples, increasing the effective population size an order of magnitude and preventing matings between potentially related queens and drones.
Miao, Jin; Reisig, Dominic D.; Li, Guoping; Wu, Yuqing
Megacopta cribraria F. (Hemiptera: Plataspidae), the kudzu bug, is an invasive insect pest of U.S. soybean. At present, insecticide application is the primary and most effective control option for M. cribraria. In this study, the potential effects of sublethal and low-lethal concentrations (LC10 and LC40) of three common insecticides on key biological traits and acetylcholinesterase (AChE) activity of the treated nymphal stage of insect were assessed. The results show that the sublethal concentration of imidacloprid significantly reduced adult emergence rate of M. cribraria. A low-lethal concentration of imidacloprid significantly increased nymphal development time, but significantly decreased adult emergence rate and adult longevity. Both sublethal and low-lethal concentrations of acephate caused an increase in nymphal development time and a reduction in adult emergence rate and adult longevity. Fecundity of females was significantly reduced only by exposure to low-lethal concentrations of acephate. Sublethal and low-lethal concentrations of bifenthrin increased nymphal development time, but significantly decreased adult emergence rate. In addition, we found that the AChE activity of M. cribraria was significantly increased only by LC40 imidacloprid, but strongly inhibited by acephate. PMID:27638957
Wellins, C A; Rittschof, D; Wachowiak, M
The ghost crab,Ocypode quadrata, was tested in the field for its ability to locate sources of volatile cues. The pure compound skatole, 3-methylindole, was a potent attractant. Crabs also located sources of complex odors such as dead fish,Lutjanus campechanus, dead mole crabs,Emerita talpoida; and peeled bananas. Ghost crabs possess concealed and reduced antennules that may not be the primary olfactory organs. Chemosensory hairs borne on the dactyls may be the primary detection system.
Aguiar, Hilton Jeferson Alves Cardoso de; Barros, Luísa Antônia Campos; Alves, Danúbia Rodrigues; Mariano, Cléa Dos Santos Ferreira; Delabie, Jacques Hubert Charles; Pompolo, Silvia das Graças
Two valid ant species, Camponotus rufipes and Camponotus renggeri, have recently been the subject of a broad discussion with reference to taxa synonymization. Both species are quite common among the Neotropical myrmecofauna and share some unique traits, such as the shape of the scape and the pilosity patterns of the tibiae and scapes. A single morphological trait can help distinguish these species; however, only a combination of different approaches can enlighten our view of the complex phylogenetic relationships prevailing in the different populations of these two taxa. Therefore, focusing on the taxonomic issues concerning these two species, a cytogenetic survey including 10 populations of C. rufipes and two populations of C. renggeri was performed. In order to better understand the extent of the relationship between C. rufipes and C. renggeri, two common Neotropical Camponotus species, C. atriceps and C. cingulatus were taken as outgroups. All four species of Camponotus that were studied had 2n = 40 chromosomes (4sm+34st+2t); however, the abundance of chromosome rearrangements observed, combined with several chromosome markers, suggest that C. rufipes and C. renggeri are two good distinct species although closely related. The already reported chromosome translocation 2n = 39 (1m+4sm+32st+2t) for C. rufipes has been found in different populations as in the unprecedented chromosome inversions found both in C. rufipes and in C. renggeri populations. Within the C. renggeri chromosome inversions, both the heterozygous state 2n = 40 (1m+3sm+34st+2t) and the homozygous state, 2n = 40 (2m+2sm+34st+2t) were identified. However, only heterozygous specimens for chromosome inversions were found among C. rufipes, with karyotype configurations distinct from those found in C. renggeri, with 2n = 40 (1m+4sm+34st+2t). None of the populations studied showed signs of mosaic individuals. With respect to rDNA clusters, the 18S rDNA seemed to be more restricted inside the genome, as C. renggeri showed four 18S rDNA clusters, whereas, C. rufipes, C. atriceps, and C. cingulatus showed only two clusters. The chromosome locations of the 5S rDNA clusters were pointed for the first time in Formicidae, and showed itself to be more widely spread over the genome. By combining different chromosome banding approaches it was possible to demonstrate the crucial importance that chromosome inversions played on the karyotype evolution within these ants. The results also showed that chromosome translocations might be a consequence of the chromatin dynamic condition observed among Camponotus species. The homozygosis condition found in a C. renggeri from a Brazilian savanna population for chromosome inversions and the contrasting heterozygous condition for a different kind of chromosome inversion in C. rufipes from the Brazilian coastal rainforest, opens the window for a chromosome race hypothesis within the group C. renggeri and C. rufipes. The wide distribution, rich ecological interactions, genetic diversity, and morphological variability among C. renggeri and C. rufipes justify questioning of the actual taxonomic status of these species. The answer of this puzzle is clear when observing the number of 18S rDNA clusters of these ants, as C. rufipes has only two clusters whereas C. renggeri has four.
Sousa, R; Pinho, M R; Delgado, J; Biscoito, M; Pinto, A R; Dellinger, T; Gouveia, L; Carvalho, D; Henriques, P
Several experimental surveys were carried out in the Northeastern Atlantic, Madeira archipelago from 1991 to 2008 to explore new fisheries resources. This study examined the selectivity of bottom and floating traps and the analysis of yield-per-recruit (YPR) and biomass-per-recruit (BPR) providing helpful insight to the management of the shrimp Plesionika narval. A total of 28,262 specimens were sampled and the analysis of length at first capture returned higher values when using floating traps indicating that these traps are more selective, exerting less pressure on the resource. The YPR and BPR analysis showed that the stock is under exploited for the studied area and suggests that the use of floating traps in the commercial fisheries of P. narval is recommended, which will allow a higher maximum allowable limit of exploitation and greater yield. The results suggest that P. narval has the potential to support a viable and sustainable fishery using floating traps.
Deguang Liu; Michael J. Bohne; Jana C. Lee; Mary Louise Flint; Richard L. Penrose; Steven J. Seybold
An overwintering North American population of the redhaired pine bark beetle (RPBB) was first discovered in November 2000 in Rochester, New York. In July 2003 it was also detected at two locations in Los Angeles County, California near heavily urbanized areas where exotic pines of Mediterranean origin are favored landscape trees. California and New York currently have...
Hammer, Karen M; Kristiansen, Erlend; Zachariassen, Karl Erik
The option of storing CO(2) in subsea rock formations to mitigate future increases in atmospheric CO(2) may induce problems for animals in the deep sea. In the present study the deep-sea bivalve Acesta excavata was subjected to environmental hypercapnia (pHSW 6.35, P(CO₂) =33,000 μatm) corresponding to conditions reported from natural CO(2) seeps. Effects on acid-base status and metabolic rate were related to time of exposure and subsequent recovery. During exposure there was an uncompensated drop in both hemolymph and intracellular pH. Intracellular pH returned to control values, while extracellular pH remained significantly lower during recovery. Intracellular non-bicarbonate buffering capacity of the posterior adductor muscle of hypercapnic animals was significantly lower than control values, but this was not the case for the remaining tissues analyzed. Oxygen consumption initially dropped by 60%, but then increased during the final stages of exposure, which may suggest a higher tolerance to hypercapnia than expected for a deep-living species. Copyright © 2011 Elsevier Ltd. All rights reserved.
Wu, Jian-Hui; Huang, Zhen; Ren, Shun-Xiang; Zhou, Hui-Ping
By using life table parameters, the effects of seven constant temperature regimes (17, 20, 23, 26, 29, 32 and 35 degrees C) on the development and reproduction of Sylepta derogata was investigated. The results indicated that the durations of egg-laying, larval, pupal, and adult stages of S. derogata shortened significantly when the temperature increased from 17 degrees C to 26 degrees C, but prolonged at 32 degrees C and 35 degrees C. The lower development threshold temperature of S. derogata at its all development stages was 12.08 degrees C, and the effective accumulative temperature was 436.2 degree-days. The survival rates of all immature stages were the highest at 26 degrees C, and would be decreased when the temperature was above or under 26 degrees C. The innate capacity to increase (rm), net reproductive rate (R0), and finite rate of increase (lambda) reached the maximum at 29, 26 and 32 degrees C, with the values being 0.1268, 415.65 and 1.185, respectively. The mean generation time (T) shortened with increasing temperature, with the maximum and minimum values of 89.11 and 28.68 days at 17 degrees C and 35 degrees C, respectively.
Dubey, A; Omkar; Mishra, G
Body melanisation in insects is polygenic, resulting from genetic polymorphism or phenotypic plasticity, with diverse implications ranging from thermal budgeting to reproductive success. In this study, we assessed the, mate choice, reproductive success, and offspring colouration of typical (T) and melanic (M) morphs of the ladybird Menochilus sexmaculatus paired at three temperatures 15°C, 25°C and 35°C. Mating success of the two morphs and the consequences for offspring fitness and offspring phenotype under these temperature regimes were evaluated. Melanic adults of both sexes achieved significantly higher mating success at 15°C and 25°C, but at 35°C no influence of adult morph on mate selection was observed. Melanic females were more fecund than typical females at all temperatures. Offspring of melanic parents developed faster than those of typicals at 15°C and 25°C, but not at 35°C. Evidence was also found of phenotypic plasticity in colour form at 15°C and 35°C. At 25°C the parents of pure (T) and (M) morphs produced offspring of the same morph. However, low temperature induced partial melanisation among the offspring of typical parents (T). Whereas at 35°C the offspring of (T) parents became paler in colour with very fine zigzag lines on elytra, i.e. they decrease the degree of melanisation. Pure melanics (M) compensated for elevated temperature stress by producing offspring that were either pure melanic but small or large with reduced melanisation. Our results on offspring phenotype variation indicate that the degree of melanism in morphs is a result of environmentally regulated expression of the parental genotype. Copyright © 2016 Elsevier Ltd. All rights reserved.
Zheng, Dongmei; Zhang, Zhongsheng; Wang, Qichao
Total and methyl mercury concentrations of cicada bodies, wings, and exuviae were investigated to study the mercury distribution characteristics. Results indicated that total and methyl mercury concentrations of cicada bodies were 2.64 mg/kg and 123.93 ng/g on average, respectively. In cicada tissues, total mercury concentrations were found to increase in the order of exuviae (0.50 mg/kg on average) < wings (0.98 mg/kg on average) < cicada bodies (2.64 mg/kg on average) and methyl mercury concentrations of cicada bodies were 123.93 ng/g on average and were the highest. Methyl mercury concentrations accounted for about 4.69% of total mercury in cicada bodies and most mercury was in inorganic forms in cicada. Sex differences of total mercury concentrations were significantly great (F = 8.433, p < 0.01) and total mercury concentrations of the males, which were 3.38 mg/kg on average, were much higher. Correlation analysis showed that neither total nor methyl mercury concentrations of cicada bodies was significantly related to the corresponding contents of soil (r = 0.0598, p > 0.05).
Beati, Lorenza; Nava, Santiago; Burkman, Erica J; Barros-Battesti, Darci M; Labruna, Marcelo B; Guglielmone, Alberto A; Cáceres, Abraham G; Guzmán-Cornejo, Carmen M; León, Renato; Durden, Lance A; Faccini, João L H
Amblyomma cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics. Total evidence analyses of the concatenated mtDNA and nuclear + mtDNA datasets resulted in well-resolved and fully congruent reconstructions of the relationships within A. cajennense. The phylogenetic analyses consistently found A. cajennense to be monophyletic and to be separated into six genetic units defined by mutually exclusive haplotype compositions and habitat associations. Also, genetic divergence values showed that these lineages are as distinct from each other as recognized separate species of the same genus. The six clades are deeply split and node dating indicates that they started diverging in the middle-late Miocene. Behavioral differences and the results of laboratory cross-breeding experiments had already indicated that A. cajennense might be a complex of distinct taxonomic units. The combined and congruent mitochondrial and nuclear genetic evidence from this study reveals that A. cajennense is an assembly of six distinct species which have evolved separately from each other since at least 13.2 million years ago (Mya) in the earliest and 3.3 Mya in the latest lineages. The temporal and spatial diversification modes of the six lineages overlap the phylogeographical history of other organisms with similar extant trans-Amazonian distributions and are consistent with the present prevailing hypothesis that Neotropical diversity often finds its origins in the Miocene, after the Andean uplift changed the topology and consequently the climate and ecology of the Neotropics.
Background Cotton worm is one of the main insects of soybean in southern China. Plants may acquire defense mechanisms that confer protection from predation by herbivores. Induced responses can lead to increased resistance against herbivores in many species. This study focuses on searching changed proteins in soybean defense response induced by cotton worm feeding. Results Ten protein spots that are changed in abundance in response to cotton worm feeding were identified by Two-dimensional gel electrophoresis (2-DE). A total of 11 unique proteins from these spots were identified by MALDI-TOF MS. The mRNA and protein relative expression levels of most changed proteins were up-regulated. These proteins were mainly involved in physiological processes, including active oxygen removal, defense signal transduction, and metabolism regulation. Conclusion This is the first proteomic analysis of the soybean defense response induced by cotton worm. The differentially expressed proteins could work together to play a major role in the induced defense response. PAL and SAMS were up-regulated at both the protein and mRNA levels. These genes can be strongest candidates for further functional research. PMID:22397523
Beaurepaire, Alexis L; Kraus, Bernard F; Koeniger, Gudrun; Koeniger, Nikolaus; Lim, Herbert; Moritz, Robin F A
The giant honeybee Apis dorsata often forms dense colony aggregations which can include up to 200 often closely related nests in the same location, setting the stage for inbred matings. Yet, like in all other Apis species, A. dorsata queens mate in mid-air on lek like drone congregation areas (DCAs) where large numbers of males gather in flight. We here report how the drone composition of A. dorsata DCAs facilitates outbreeding, taking into the account both spatial (three DCAs) and temporal (subsequent sampling days) dynamics. We compared the drones’ genotypes at ten microsatellite DNA markers with those of the queen genotypes of six drone-producing colonies located close to the DCAs (Tenom, Sabah, Malaysia). None of 430 sampled drones originated from any of these nearby colonies. Moreover, we estimated that 141 unidentified colonies were contributing to the three DCAs. Most of these colonies were participating multiple times in the different locations and/or during the consecutive days of sampling. The drones sampled in the DCAs could be attributed to six subpopulations. These were all admixed in all DCA samples, increasing the effective population size an order of magnitude and preventing matings between potentially related queens and drones. PMID:25558361
Blackledge, Todd A; Hayashi, Cheryl Y
Orb-weaving spiders spin five fibrous silks from differentiated glands that contain unique sets of proteins. Despite diverse ecological functions, the mechanical properties of most of these silks are not well characterized. Here, we quantify the mechanical performance of this toolkit of silks for the silver garden spider Argiope argentata. Four silks exhibit viscoelastic behaviour typical of polymers, but differ statistically from each other by up to 250% in performance, giving each silk a distinctive suite of material properties. Major ampullate silk is 50% stronger than other fibers, but also less extensible. Aciniform silk is almost twice as tough as other silks because of high strength and extensibility. Capture spiral silk, coated with aqueous glue, is an order of magnitude stretchier than other silks. Dynamic mechanical properties are qualitatively similar, but quantitatively vary by up to 300% among silks. Storage moduli are initially nearly constant and increase after fiber yield, whereas loss tangents reach maxima of 0.1-0.2 at the yield. The remarkable mechanical diversity of Argiope argentata silks probably results in part from the different molecular structures of fibers and can be related to the specific ecological role of each silk. Our study indicates substantial potential to customize the mechanics of bioengineered silks.
Previous studies have investigated the interaction of different light sources and light intensity. Studies are lacking concerning the effect of different light sources and photoperiods on broiler growth and health. The results reported here are a part of a larger study to evaluate the interaction of...
Fernandes, Jose Antonio Marin; Silva, Valeria Juliete Da; Correia, Andre Oliveira; Nunes, Benedito Mendes
The edessines from Costa Rica are little known; only 18 species have been registered or described from this country so far. Mainly based in a large sample from Instituto Nacional de Biodiverdidad (INBio), Costa Rica, we decided to update the information concerning Edessinae from Costa Rica. We present a list of species from Costa Rica raising the number of known species from Costa Rica to 65. We are also describing nine new species: Edessa bella Fernandes & Silva, E. bruneolineata Fernandes & Correia, E. curvata Fernandes & Nunes, E. lewisi Fernandes & Silva, E. nigroangulata Fernandes & Silva, E. osae Fernandes & Nunes, E. oxcarti Fernandes & Correia, E. pallidoangulata Fernandes & Nunes and E. puravida Fernandes & Correia. Species were described, illustrated and photographed. Distribution maps for the species are also provided.
Yan, Chao; Zhang, Zhi-Xiang; Xu, Han-Hong
The present study revealed differentially expressed genes responsive to azadirachtin A (Aza) in Spodoptera litura cell line through suppression subtractive hybridization. In the Aza-responsive SSH library, approximately 270 sequences represent 53 different identified genes encoding proteins with various predicted functions, and the percentages of the gene clusters were 26.09% (genetic information processing), 11.41% (cell growth and death), 7.07% (metabolism), 6.52% (signal transduction/transport) and 2.72% (immunity), respectively. Eleven clones homologous to identified genes were selected to be confirmed through quantitative real time polymerase chain reaction. Among the eleven clones validated, all but one transcript of lipase showed an increase in SL cell line collected from ETA, whereas the transcripts of other genes were lower in the SL cell line collected from ETA compared with that of UETA. These genes were considered to be related to the response of SL cell line to Aza. These will provide a new clue to uncover the molecular mechanisms of Aza acting on SL cell line.
This work reports on the use of cultural practices to influence grasshoppers nymphal emergence. Grasshopper eggs were buried at depths of 2, 14, 18, 22, and 26 cm in laboratory arenas. Nymph eclosion ranges from 77 to 87%. However, nymph emergence, measured as the number of nymphs that reached the s...
Sprawka, Iwona; Goławska, Sylwia; Goławski, A; Czerniewicz, P; Sytykiewicz, H
The insecticidal activity of plant lectins against a wide range of insect species have been intensively studied. Understanding the mechanism of the toxicity of lectins is one of the studied aspects. In the present research, the first step was determine the effect of phytohemagglutinin (PHA) on the development, fecundity and mortality of grain aphid. Next, the effect of PHA lectin on the activity of such enzymes as: α- and β-glucosidases, alkaline (AkP) and acid (AcP) phosphatases, aminopeptidase N and cathepsin L involved in the metabolism of sugar, phosphorus and proteins of an adult apterae aphids was investigated. The PHA lectin added into the liquid diet increased the pre-reproductive period, mortality of Sitobion avenae, the time of generation development and decreased its fecundity and the intrinsic rate of natural increase. In addition, activity of α-glucosidase, alkaline phosphatase and aminopeptidase N of adult apterae exposed to PHA were reduced. The results indicate that the insecticidal activity of PHA on S. avenae may involve changes in activity of the enzymes in the midgut and it may be part of its toxicity.
Mayrand; Guderley; Dutil
This study examined the relationships between muscle growth rate, the activity of metabolic enzymes and the RNA:DNA ratio, in adult snow crabs Chionoecetes opilio. After moulting, crabs were assigned to three feeding rations to attain a range of tissue growth rates. Muscle growth rate, estimated by the variation in dry tissue content per ml of merus of the first walking leg, was positively correlated with changes in muscle cell number, as evaluated by the DNA content per ml of merus. However, no significant correlation was detected between growth rate and the variation in muscle cell size, the latter being estimated by the change in the protein:DNA ratio. This is due to the fact that, in starved crabs, a reduction in the number of cells is partly compensated by a size increment of the remaining ones. This phenomenon also weakened the overall relationship between muscle growth rate and the phosphofructokinase (PFK) capacity per ml of merus. The simple correlation between those two variables was significantly positive for animals which increased their mass of muscle but insignificant for those which were loosing muscle mass. The lactate dehydrogenase (LDH), citrate synthase (CS) and cytochrome c oxidase (CCO) capacity per ml of merus did not match growth rate. The significant simple correlations that were detected between growth rate and the various enzyme activity expressed per g of protein, per µg of DNA and per g of dry mass did not hold when partial correlations were computed. Variations in muscle cell size were related to adjustments in the quantity of RNA per cell, as depicted by the RNA:DNA ratio. Since muscle growth was not correlated with the variation in muscle cell size, it was not correlated with the RNA:DNA ratio either.
Fabricius, C.; Makarov, V. V.
The table contains the Hipparcos (Cat. ) identification number, the number of components photometrically resolved for each system, component identification letter, Hp magnitude as it is given in the Hipparcos Double and Multiple Systems Annex (DMSA) and its standard error, observed Tycho BT and VT magnitudes and their formal standard errors, and observed signal-to-noise ratio for each component star. The reference component identification, relative separation and position angle with respect to the reference component are also given for secondary component stars. A total of 5173 double and multiple systems with separations above 0.3arcsec are resolved into 9473 components. (1 data file).
Andrade, L S; Goés, J M; Fransozo, V; Alves, D F R; Teixeira, G M; Fransozo, A
The structurally diverse rocky shores along the northern coast of the state of São Paulo, Brazil, support a varied fauna and provide refuges for many organisms. Some of these environments allow for extensive microhabitats, among them the sand reefs formed by the polychaete Phragmatopoma lapidosa, which occupy much of this area. The beauty of the landscape attracts large numbers of tourists, who contribute to the damage to the sand reef colonies, causing an imbalance in the patterns of population distribution and of this ecosystem. We describe the structure and population biology of the redfinger rubble crab Eriphia gonagra, and investigated the differential occupation of the habitat by each demographic category of this species. Crabs were sampled monthly for two consecutive years on the rocky coast of Grande Beach, Ubatuba, São Paulo, during spring low tides. Sampling was carried out over an area of approximately 1200 m2, during two hours on the rock surface and another two hours on the sand reefs. A total of 1407 crabs were collected; 776 on the sand reef (SR) and 631 on the rocky shore (RO). The majority of juvenile crabs inhabited the SR, while adult crabs were equally distributed in both microhabitats. This study showed that the SR is a natural nursery ground for the establishment of the early juvenile stages of E. gonagra, which use the reefs as a refuge and food resource. Many other organisms (mollusks, echinoderms, polychaetes etc.) settle on the reefs, and these areas may be among the most important in maintaining benthic diversity in the region.
Asadi, N; Rahimi, A; Ghaheri, M; Kahrizi, D; Bagheri Dehbaghi, M; Khederzadeh, S; Banabazi, M H; Esmaeilkhanian, S; Veisi, B; Geravandi, M; Karim, H; Vaziri, S; Daneshgar, F; Zargooshi, J
Apis florea is one of two species of small, wild honeybee. The present study was conducted to evaluate the genetic diversity of Apis florea honeybee from 48 nests (colonies) using microsatellite markers in the South of Iran. All honeybee samples were analyzed for six microsatellite loci (A88, A107, A7, B124, A113 and A35). The six loci had different numbers of alleles in the sampled colonies ranging from 7 (loci A107) to 3 (loci A7, A35). Gene diversity in Apis florea ranged from 0.491 to 0.595. This range probably reflects the spreading of nests in a large region with a varied climate. Phylogenetic tree showed two distinct clusters including a) Minab region samples and b) Bandar Abbas, Bandar Khamir and Qeshm Island regions. All of these regions are geographically rich, having varied vegetation and climate conditions. Our findings are an important contribution to the methods of studying distribution and conservation of Apis florea.
Background Amblyomma cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics. Results Total evidence analyses of the concatenated mtDNA and nuclear + mtDNA datasets resulted in well-resolved and fully congruent reconstructions of the relationships within A. cajennense. The phylogenetic analyses consistently found A. cajennense to be monophyletic and to be separated into six genetic units defined by mutually exclusive haplotype compositions and habitat associations. Also, genetic divergence values showed that these lineages are as distinct from each other as recognized separate species of the same genus. The six clades are deeply split and node dating indicates that they started diverging in the middle-late Miocene. Conclusions Behavioral differences and the results of laboratory cross-breeding experiments had already indicated that A. cajennense might be a complex of distinct taxonomic units. The combined and congruent mitochondrial and nuclear genetic evidence from this study reveals that A. cajennense is an assembly of six distinct species which have evolved separately from each other since at least 13.2 million years ago (Mya) in the earliest and 3.3 Mya in the latest lineages. The temporal and spatial diversification modes of the six lineages overlap the phylogeographical history of other organisms with similar extant trans-Amazonian distributions and are consistent with the present prevailing hypothesis that Neotropical diversity often finds its origins in the Miocene, after the Andean uplift changed the topology and consequently the climate and ecology of the Neotropics. PMID:24320199
Cox, D.K.; Beauchamp, J.J.
Juvenile crayfish, Cambarus bartoni, were subjected to acute thermal changes after being acclimated to temperatures of 15, 20 or 25 C for at least 1 week. Groups of 10 individuals each were exposed to various combinations of time and temperature, after which they were returned to their original acclimation temperature. Observations of latent mortality made 72 hr later indicated that some crayfish experience lethal temperature stress between 30 and 33 C; their ultimate upper incipient lethal temperature is estimated to be 32.5 C. A linear logistic model was used to construct contours of constant mortality (30, 50 and 90%) as a function of time and temperature.
Cox, D.K.; Beauchamp, J.J.
Juvenile crayfish, Cambarus bartoni, were subjected to acute thermal changes after being acclimated to temperatures of 15, 20 or 25 C for at least 1 week. Groups of 10 individuals each were exposed to various combinations of time and temperature, after which they were returned to their original acclimation temperature. Observations of latent mortality made 72 hr later indicated that some crayfish experience lethal temperature stress between 30 and 33 C; their ultimate upper incipient lethal temperature is estimated to be 32.5 C. A linear logistic model was used to construct contours of constant mortality (30, 50 and 90%) as a function of time and temperature.
Zia, S.; Alikhan, M.A.
Large amounts of acid forming sulfur dioxide, and heavy metals including copper, are continuously being released into the environment by mining and smelting operation at Sudbury, Ontario, Canada. Consequently, a number of lakes in this region have become acidic and metal stressed. In the current study the uptake and accumulation of copper by various tissues of a copper-tolerant crayfish, Cambarus bartoni, were monitored in the laboratory to ascertain the dynamic nature (i.e., the pattern in time) of responses of crayfish to increased levels of these two metals in the water.
Alikhan, M.A.; Zia, S.
Large amounts of acid forming sulfur dioxide, and heavy metals including nickel are continuously being released into the environment by mining and smelting operations at Sudbury, Ontario, Canada. As a consequence, a number of lakes in this region has become acidic and metal stressed. In the current study the uptake and accumulation of nickel by various tissues of a copper-tolerant crayfish, Cambarus bartoni (Decapod, Crustacea), was monitored for 4 wk in the laboratory to ascertain the dynamic nature (i.e., the pattern in time) of the response of the crayfish to increased levels of this relatively less metabolically essential but toxic metal in the aquatic environment.
Singh, Satya; Kumar, Krishna
Treatment of early and late third instar larvae of economically important insect C. megacephala with the ageratochromene precocene II resulted into precocious metamorphosis. The other effects included mortality, production of pupal-adult mosaics, reduction in percentage of adult emergence and formation of non-viable deformed adults that died after a few hours of emergence. The application of precocene II adversely hampered the normal growth, development and metamorphosis of the insect and the results show that precocene II has vast potential for excellent control of this insect of medico-veterinary importance and can be used in insect pest management programs.
Ardika, Puji Utari; Farajallah, Achmad; Wardiatno, Yusli
Specimens of Hippa adactyla (Crustacea, Anomura, Hippidae) were collected from several coasts of Indonesia (Sumatera, Java, Bali-Lombok and Sulawesi). This finding represents the first record of this species in Indonesia and confirms its presence in the Indian Ocean and in the Wallacea region. Its systematic and morphological characteristics are described, and its distribution in Indonesia is presented. One of the main characteristics of this species is a median lobe in the anterior part of the carapace, which has 3–4 lobes. Likewise, the left antenna has 2–6 articles. PMID:26868713
Ardika, Puji Utari; Farajallah, Achmad; Wardiatno, Yusli
Specimens of Hippa adactyla (Crustacea, Anomura, Hippidae) were collected from several coasts of Indonesia (Sumatera, Java, Bali-Lombok and Sulawesi). This finding represents the first record of this species in Indonesia and confirms its presence in the Indian Ocean and in the Wallacea region. Its systematic and morphological characteristics are described, and its distribution in Indonesia is presented. One of the main characteristics of this species is a median lobe in the anterior part of the carapace, which has 3-4 lobes. Likewise, the left antenna has 2-6 articles.
Faraldo, A C; Gregório, E A; Lello, E
Insects manifest effective immune responses that include both cellular and humoral components. Morphological and quantitative aspects of cellular and humoral cooperation during nodule formation in Chrysomya megacephala hemolymph against Saccharomyces cerevisae yeast cells were demonstrated for the first time. The analyses were performed in non-injected larvae (NIL), saline-injected larvae (SIL) and yeast-injected larvae (YIL). The hemolymph of injected groups was collected 0.5, 1, 2, 4, 12, 24, 36, or 48-h post-injection. Morphological aspects of YIL nodulation were investigated using transmission electron microscopy (TEM). Quantitative analyses consisted of total (THC) and differential hemocyte counts (DHC) in all the groups and total yeast count (TYC) in YIL, which were performed in an improved Neubauer chamber. Nodule formation was initiated at approximately 2-h post-injection. Twelve hours after the injection, TEM revealed the presence of an amorphous membrane, at the same time that circulating hemocyte number decreased significantly contrasting the increase of yeast number. Our results showed the ability of C. megacephala hemolymph to perform humoral encapsulation when hemocyte population is insufficient to eliminate the microorganisms, warranting consideration in future investigations on the relative roles played by cellular and humoral elements of innate immunity of this calliphorid.
Ferraz, Adriana C P; Gadelha, Bárbara Q; Aguiar-Coelho, Valéria M
Monthly collections were made using two traps 5 m apart exposed for 48h, containing sardines and installed at points: A at the edge (500 m from the entrance of the Reserve); B 1200 m from the entrance and 1000 m inside the forest; and C 1700 m from the entrance and 500 m inside the forest. The purpose was to evaluate the abundance and richness of calliphorid species as a function of the environmental conditions using Pearson's correlation, compare the richness of the areas using ANOVA and Tukey's test, compare the abundances of the areas by the Kruskal-Wallis test, and also assess the possible influence of the anthropic presence. Rare, intermediary and common species were identified. The collection totalized 8515 Calliphoridae belonging to 26 species, with a predominance of females. None of the 13 species considered common presented a correlation between abundance and temperature: only Cochliomyia hominivorax (Coquerel) and Chrysomya megacephala (Fabricius) were correlated with humidity and only Mesembrinella semihyalina Mello with precipitation. This parameter was the only climatic variable correlated with richness. The greatest abundance and richness of calliphorids occurred in September 2006. From the 13 common species, seven were considered synanthropic, indicating the effect of anthropic action in this site.
Laranja, Joseph Leopoldo Q; Ludevese-Pascual, Gladys L; Amar, Edgar C; Sorgeloos, Patrick; Bossier, Peter; De Schryver, Peter
Low larval survival resulting from suboptimal culture conditions and luminous vibriosis poses a major problem for the larviculture of penaeid shrimp. In this study, a poly-β-hydroxybutyrate (PHB) accumulating mixed bacterial culture (mBC; 48.5% PHB on cell dry weight) and two PHB accumulating bacterial isolates, Bacillus sp. JL47 (54.7% PHB on cell dry weight) and Bacillus sp. JL1 (45.5% PHB on cell dry weight), were obtained from a Philippine shrimp culture pond and investigated for their capacity to improve growth, survival and robustness of Penaeus monodon postlarvae (PL). Shrimp PL1 and shrimp PL30 were provided with the PHB containing bacterial cultures in the feed for 30 days followed by, respectively, a challenge with pathogenic Vibrio campbellii and exposure to a lethal dose of ammonia. Prior to the pathogenic challenge or ammonia stress, growth and survival were higher for shrimp receiving the PHB accumulating bacteria as compared to shrimp receiving diets without bacterial additions. After exposure to the pathogenic challenge the shrimp fed PHB accumulating bacteria showed a higher survival as compared to non-treated shrimp, suggesting an increase in robustness for the shrimp. Similar effects were observed when shrimp PL30 were provided with the PHB accumulating bacterial cultures during a challenge with pathogenic V. campbellii through the water. The survival of shrimp exposed to lethal ammonia stress showed no significant difference between PHB accumulating bacteria-fed shrimp and non-PHB treated shrimp. The data illustrate that bacilli capable of accumulating PHB can provide beneficial effects to P. monodon post-larvae during culture in terms of growth performance, survival and resistance against pathogenic infection and ammonia stress. Further investigations are required to verify the PHB effect of the bacterial cultures on the shrimp.
Lu, Y-H; Zheng, X-S; Gao, X-W
The aphid species Sitobion avenae and Rhopalosiphum padi are the most important pests in wheat growing regions of many countries. In this study, we investigated the sublethal effects of imidacloprid on fecundity, longevity, and enzyme activity in both aphid species by comparing 3-h exposure for one or three generations. Our results indicated that 3-h exposure to sublethal doses of imidacloprid for one generation had no discernible effect on the survival, fecundity, longevity, or enzyme activity levels of aphids. However, when pulse exposures to imidacloprid were sustained over three generations, both fecundity and longevity were significantly decreased in both S. avenae and R. padi. Interestingly, the fecundity of R. padi had almost recovered by the F5 generation, but its longevity was still deleteriously affected. These results indicated that R. padi laid eggs in shorter time lags and has a more fast resilience. The change in reproduction behavior may be a phenomenon of R. padi to compensate its early death. If this is stable for the next generation, it means that the next generation is more competitive than unexposed populations, which could be the reason underlying population outbreaks that occur after longer-term exposure to an insecticide. This laboratory-based study highlights the sublethal effects of imidacloprid on the longevity and fecundity of descendants and provides an empirical basis from which to consider management decisions for chemical control in the field.
ScathL is a cathepsin L-like cysteine protease derived from the flesh fly Sarcophaga peregrina that functions in basement membrane (BM) remodeling during insect development. A recombinant baculovirus expressing ScathL (AcMLF9.ScathL) kills larvae of the tobacco budworm, Heliothis virescens, signific...
Antunes, Graziani de Freitas; do Amaral, Ana Paula Nunes; Ribarcki, Fabiana Pinto; Wiilland, Elenir de Fátima; Zancan, Denise Maria; Vinagre, Anapaula Sommer
The ghost crab, Ocypode quadrata, is found on sandy beaches from the United States to Southern Brazil. Because there is still little information about the metabolism and reproduction of O. quadrata on the southern coast of Brazil, the objectives of this study were to (i) evaluate the effect of seasonal variations on the carbohydrate, lipid, and protein metabolism of O. quadrata at Rondinha Beach, a beach with high anthropogenic activity, and to compare it with data from Siriú Beach, which has less human activity; and (ii) describe the effect of seasonal variations on the histological characteristics of male and female gonads, in order to assess the reproductive capacity of the crabs. The gonads of male crabs showed no significant variations in the gonadosomatic index (GSI) and glycogen levels; however, histological analysis of the testes revealed that they are mature in the summer. In females, the GSI and glycogen values are higher in summer, concomitantly with the presence of mature oocytes. These results suggest that the reproductive peak of O. quadrata occurs in summer. The seasonal analysis of the biochemical parameters, as well as comparison with Siriú Beach, demonstrated that the ghost crabs of Rondinha Beach have a different pattern of metabolism than those of Siriú. This difference may be a consequence of differences in the environmental conditions as well as in the anthropogenic pressures, such as vehicle traffic and the increase in human population at the beach in summer.
Oyedokun, A.V.; Adeniyi, D.O.
The cashew stem girdler, Analeptes trifasciata, is a major insect pest of cashew in Nigeria causing economic damage in cashew plantations even at low density. In this study, newly emerged adults of A. trifasciata reared from field-infested cashew stems were collected from the rearing cages, sexed, and dissected to reveal the internal structures of the insects. The gut was excised and separated into the foregut, midgut, and hindgut. The dissected gut compartments were blotted dry by sandwiching in sterile Whatman No. 1 (150 mm) filter paper for a minute. The inoculated gut parts showed the presence of eight fungi flora, namely, Aspergillus repens, Trichoderma spp., Fusarium verticillioides, Lasiodiplodia theobromae, yeast, Aspergillus niger, Fusarium spp., and Rhizopus stolonifer. The frequencies of occurrence of bacteria in the gut compartments of A. trifasciata were Enterobacter spp.: 83.33%; Escherichia coli and Streptococcus spp.: 55.56% each; Staphylococcus spp.: 44.44%; Klebsiella pneumonia: 50% and Salmonella shigella: 11.11%, while each of Serratia marceascea, Pseudomonas spp., and Micrococcus lutea had 5.56% occurrence. The occurrence of mycoflora and microbiota species varied in the gut compartments of A. trifasciata, indicating the role of these microorganisms in metabolic and other bioprocesses of A. trifasciata during digestion and synthesis of complex food substances from the cashew stem substrate. This study would provide basic information for enzymatic studies of A. trifasciata with a view to developing an integrated pest management (IPM) protocol for managing the pest in cashew plantations. PMID:27147898
Proshchalykin, Maxim Yu; Dathe, Holger H
As a supplement to a previously published study on Siberian Hylaeus species, we here report further records of twenty six rarely collected and little known species. Thirty two species are currently known from Siberia, including Hylaeus dorni Dathe, 1986, H. gredleri Förster, 1871, H. hungaricus (Alfken, 1905), H. moricei (Friese, 1898), H. nimbatus Dathe, 1986, H. oehlkei Dathe, 2010 and H. stubbei Dathe, 1986, which are reported from Russia for the first time, and H. brevicornis Nylander, 1852, H. communis Nylander, 1852, H. pfankuchi (Alfken, 1919) and H. styriacus Förster, 1871, which are newly recorded from Siberia. Hylaeus (Hylaeus) pesenkoi Proshchalykin & Dathe, sp. nov. is described as new from Siberia (Altai Republic and Tyva Republic) and neighbouring Mongolia (Khovd and Govi-Altai Aimags). The females of H. dorni Dathe, 1986 and H. oehlkei Dathe, 2010 are described for the first time; H. peregrinus Dathe, 1986 was recognised as the female of H. stubbei Dathe, 1986 and is treated as a new synonym.
Fuller, Pam L.; Knott, David M.; Kingsley-Smith, Peter R.; Morris, James A.; Buckel, Christine A.; Hunter, Margaret E.; Hartman, Leslie D.
After going unreported in the northwestern Atlantic Ocean for 18 years (1988 to 2006), the Asian tiger shrimp, Penaeus monodon, has recently reappeared in the South Atlantic Bight and, for the first time ever, in the Gulf of Mexico. Potential vectors and sources of this recent invader include: 1) discharged ballast water from its native range in Asia or other areas where it has become established; 2) transport of larvae from established non-native populations in the Caribbean or South America via ocean currents; or 3) escape and subsequent migration from active aquaculture facilities in the western Atlantic. This paper documents recent collections of P. monodon from the South Atlantic Bight and the Gulf of Mexico, reporting demographic and preliminary phylogenetic information for specimens collected between North Carolina and Texas from 2006 through 2012. The increased number of reports in 2011 and 2012, ranging from 102 mm to 298 mm total length, indicates that an adult population is present in densities sufficient for breeding, which is indicative of incipient establishment. Based on these reports of P. monodon, its successful invasion elsewhere, and its life history, we believe that this species will become common in the South Atlantic Bight and Gulf of Mexico in less than 10 years. Penaeus monodon is an aggressive predator in its native range and, if established, may prey on native shrimps, crabs, and bivalves. The impacts of an established P. monodon population are potentially widespread (e.g., alterations in local commercial fisheries, direct and indirect pressures on native shrimp, crab and bivalve populations, and subsequent impacts on the populations of other predators of those organisms) and should be considered by resource managers. The impacts of P. monodon on native fauna and the source(s) or vector(s) of the invasion, however, remain unknown at this time.
Bacci, Maurício; Solomon, Scott E; Mueller, Ulrich G; Martins, Vanderlei G; Carvalho, Alfredo O R; Vieira, Luiz G E; Silva-Pinhati, Ana Carla O
Leafcutting ants of the genus Atta are the most conspicuous members of the tribe Attini, the fungus-growing ants. Atta species have long attracted the attention of naturalists, and have since become a common model system for the study of complex insect societies as well as for the study of coevolutionary dynamics due to their numerous interactions with fungi and other microbes. Nevertheless, systematics and taxonomy of the 15 species in the genus Atta have proven challenging, due in part to the extreme levels of worker polymorphism these species display, leading to disagreements about the validity of as many as five different subgenera and calling into question the monophyly of the genus. Here, we use DNA sequence information from fragments of three mitochondrial genes (COI, tRNA leucine and COII) and one nuclear gene (EF1-alphaF1), totaling 1070 base pairs, to reconstruct the phylogenetic relationships of Atta species using maximum parsimony, maximum likelihood and Bayesian inference techniques. Our results provide support for monophyly of the genus Atta, and suggest that the genus is divided into four monophyletic groups, which correspond to four of the five previously erected Atta subgenera: Atta sensu stricto and Archeatta, each with species composition identical to earlier proposals; Neoatta and Epiatta, with major differences in species composition from earlier proposals. The current geographic ranges of these species suggest that the historical separation of South America from Central and North America has played a role in speciation within this genus.
Bergamaschi, Antonio C.B.; Lama, Marco A. Del
Abstract Trypoxylon is a genus of solitary crabronid wasps whose population genetics is poorly known. The purpose of the present study was to investigate the genetic variation and differentiation among five populations of Trypoxylon albitarse, a species widely distributed throughout the Neotropics, with records from Panama to northern Argentina. Eight species-specific microsatellite loci were used for genotyping 96 adult wasps (one female per nest) sampled at five sites in Brazil. The analysis of allelic richness and private alleles indicated high genetic diversity in the populations sampled. Pairwise comparisons using the F st and D est indices revealed significant differentiation for all, but one pair of populations. F st, D est, AMOVA and assignment test values pointed to inter-population differentiation. Additionally, the analysis of population structure using Bayesian and PCA methods characterized two alternative genetic groups. The Mantel test indicated no correlation between genetic and geographic distances. Despite evidence of considerable dispersal capacity for T. albitarse, the data indicate low to moderate population structuring in this species. PMID:26692160
Carramaschi, Isabel Nogueira; Pereira, Lorrane de Andrade; Queiroz, Margareth Maria de Carvalho; Zahner, Viviane
This study evaluated whether different strains of Brevibacillus laterosporus could be used to control larvae of the blowfly Chrysomya megacephala, a pest that affects both human and animal health. Mortality rates were recorded after 1-mL suspensions of sporulated cells of 14 different strains of B. laterosporus were added to 2.5g of premixed diet consisting of rotting ground beef fed to first instar larvae of C. megacephala. All bioassays were performed using 10 larvae per strain, with a minimum of three replicates for each bioassay. Larval mortality was recorded daily up to seven days. Strains Bon 707, IGM 16-92, and Shi 3 showed the highest toxicity toward the larvae producing 70.5%, 64.5%, and 51.6% of larval mortality, respectively, which was significantly higher than that in the control group (p < 0.05). In contrast, strains NRS 1642, NRS 661, NRS 590 BL 856, NRS 342, ATCC 6457, Bon 712, and NRS 1247 showed limited or no pathogenic activity against the target larvae. Our preliminary data indicated that B. laterosporus could be used to develop bioinsecticides against C. megacephala.
Sun, Hongyan; Liu, Peng; Nolan, Lisa K.; Lamont, Susan J.
Extraintestinal pathogenic Escherichia coli (ExPEC) has major negative impacts on human and animal health. Recent research suggests food-borne links between human and animal ExPEC diseases with particular concern for poultry contaminated with avian pathogenic E. coli (APEC), the avian ExPEC. APEC is also a very important animal pathogen, causing colibacillosis, one of the world’s most widespread bacterial diseases of poultry. Previous studies showed marked atrophy and lymphocytes depletion in the bursa during APEC infection. Thus, a more comprehensive understanding of the avian bursa response to APEC infection will facilitate genetic selection for disease resistance. Four-week-old commercial male broiler chickens were infected with APEC O1 or given saline as a control. Bursas were collected at 1 and 5 days post-infection (dpi). Based on lesion scores of liver, pericardium and air sacs, infected birds were classified as having mild or severe pathology, representing resistant and susceptible phenotypes, respectively. Twenty-two individual bursa RNA libraries were sequenced, each yielding an average of 27 million single-end, 100-bp reads. There were 2469 novel genes in the total of 16,603 detected. Large numbers of significantly differentially expressed (DE) genes were detected when comparing susceptible and resistant birds at 5 dpi, susceptible and non-infected birds at 5 dpi, and susceptible birds at 5 dpi and 1 dpi. The DE genes were associated with signal transduction, the immune response, cell growth and cell death pathways. These data provide considerable insight into potential mechanisms of resistance to ExPEC infection, thus paving the way to develop strategies for ExPEC prevention and treatment, as well as enhancing innate resistance by genetic selection in animals. PMID:26556806
Araujo, Ricardo N; Costa, Fernanda S; Gontijo, Nelder F; Gonçalves, Teresa C M; Pereira, Marcos H
The bedbugs Cimex lectularius and Cimex hemipterus are obligate hematophages in all their nymphal instars as well as in the adult stage. The efficiency with which the insects obtain blood from their hosts is directly related to their population dynamics. In the present study we compared the feeding process and salivary content in individuals of these two species when fed on different blood sources or host sites, using a cibarial pump electromyogram. Females ingested more blood than males but needed longer contact time with the host to complete the meal. The bedbug C. lectularius was more efficient than C. hemipterus in obtaining blood from mice and pigeons. With regard to the feeding site on mice, it was easier for the insects to obtain blood from the skin of the belly than that of the back. Individuals of C. hemipterus were able to maintain the cibarial pump functioning at higher frequencies for longer periods when fed on pigeons treated with anticoagulant. Although saliva from C. lectularius contained more hemeproteins and showed more anti-clotting activity its total protein content was similar to that of C. hemipterus. Overall, C. lectularius obtains a bloodmeal more efficiently from its hosts, which may have enabled this species to reach higher levels of infestation than C. hemipterus.
Kavitha, R; Tan, T C; Lee, H L; Nazni, W A; Sofian, A M
DNA identification of blow fly species can be a very useful tool in forensic entomology. One of the potential benefits that mitochondrial DNA (mtDNA) has offered in the field of forensic entomology is species determination. Conventional identification methods have limitations for sibling and closely related species of blow fly and stage and quality of the specimen used. This could be overcome by DNA-based identification methods using mitochondrial DNA which does not demand intact or undamaged specimens. Mitochondrial DNA is usually isolated from whole blow fly and legs. Alternate sources for mitochondrial DNA isolation namely, egg, larva, puparium and empty puparium were explored in this study. The sequence of DNA obtained for each sample for every life cycle stage was 100% identical for a particular species, indicating that the egg, 1st instar, 2nd instar, 3rd instar, pupa, empty puparium and adult from the same species and obtained from same generation will exhibit similar DNA sequences. The present study also highlighted the usefulness of collecting all life cycle stages of blow fly during crime scene investigation with proper preservation and subsequent molecular analysis. Molecular identification provides a strong basis for species identification and will prove an invaluable contribution to forensic entomology as an investigative tool in Malaysia.
Viscuso, Renata; Federico, Concetta; Saccone, Salvatore; Bonaccorsi, Bianca; Vitale, Danilo G M
A study by fluorescence microscopy has been carried out on male gametes from testicular follicles, seminal vesicles, spermatophores, and seminal receptacles of the bush-cricket Tylopsis liliifolia, focusing the attention on localization and movements of F-actin and α-tubulin during sperm differentiation, since data in this respect are lacking in the Orthoptera. F-actin and α-tubulin positivity was detected in the testicular follicles, in particular at the bridges connecting spermatids of a same clone and around their nucleus, during the first differentiation stages. During the following differentiation stages in the testes, F-actin was found at one of the spermatid poles and then, during nucleus elongation, at the whole acrosomal region. A peculiar F-actin-positivity was found at the flagellum, more markedly immediately posterior to the nucleus, at the basal body region of the gametes from the testicular follicles and from the other examined districts. Other interesting data from our investigations concerns the α-tubulin displacements during the differentiation stages of the spermatid and a constant absence of α-tubulin-positivity where the centrioles are located. No positivity was also found for both α-tubulin and nuclear markers at the anterior region of the gamete, where the acrosomal wings are localized. Our results, compared with what is so far known in literature for the insects, lead us to assert that microfilaments and microtubules undergo gradual displacements, markedly in the testicular follicles, during the morphogenesis of the male gamete of T. liliifolia aimed to its organization and motility and probably also to its interaction with the female gamete. © 2015 Wiley Periodicals, Inc.
Khoa, L V; Hatai, K; Aoki, T
Fusarium incarnatum was isolated from gill lesions of cultured black tiger shrimp, Penaeus monodon, in every crop during 2000-2002 in Nghe An province, Vietnam. Infected shrimps showed typical signs of black gill disease and mortalities about a month prior to harvest. Detailed morphological examinations, as well as molecular phylogenic analyses based on partial nucleotide sequences of ribosomal DNA, were made on the isolates. An artificial infection of kuruma prawn, Penaeus japonicus, using two selected isolates was also conducted and their pathogenicity determined.
Dolfi, A; Lupetti, M; Bianchi, F; Michelucci, S
The aim of the present experiment was to verify whether the origin of the DIA is ectodermal or endodermal. A rabbit serum against the epithelial cells of the final portion of the cloaca was prepared. The indirect immunofluorescence method was applied to strips obtained with a cryostat, carefully cut in such a way as to include a part of the cloaca, the burso-cloacal stalk, the DIA, and the bursal plicae. In this way, it was possible to demonstrate that the epithelium of the cloaca, of the burso-cloacal stalk, and of the DIA exhibited an intense fluorescence that could not be observed at the level of the epithelium of the bursal plicae. These findings would appear to indicate that the DIA, like the cloaca and the burso-cloacal stalk, is ectodermal in origin. Furthermore, histological study revealed that the DIA exhibits close structural analogies with the dorsal wall of the cloaca. In both areas, unorganized lymphoid infiltrations of the tunica propria can be seen, and the epithelium does not show any follicle-associated epithelial cells. The glands often assume the aspect of dilated crypts containing intestinal transit material. The epithelium of these glands reveals lymphoid infiltrations at various points, and it is not uncommon to detect accumulations of cells in their lumina. Several groups of eosinophilic granulocytes can also be observed in the tunica propria of these two areas, with a clear predominance at the level of the DIA. These similarities between the cloaca and the DIA might lead one to suppose the existence of a functional as well as a morphological correspondence. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 Fig. 8 Fig. 9 Fig. 10 Fig. 11 Fig. 12 PMID:3417544
Barros-Alves, Samara De P; Alves, Douglas F R; Bolla, Eduardo Antônio; Cobo, Valter José; Negreiros-Fransozo, Maria Lucia
The first zoeal stages of ?Cataleptodius parvulus and Xanthodius denticulatus are described and compared with zoeae of other members of the subfamily Xanthinae. The larvae of ?C. parvulus and X. denticulatus differ mainly in: 1) spinulation of rostral and dorsal spines; 2) type of antenna; 3) setation of the basis of first maxilliped; and 4) type of telson. Features common to both species include a carapace provided with well-developed spines on the dorsal (1), rostral (1), and lateral (2) portions; and the antennal protopod and rostrum similar in length. The morphological differences between the zoea I of X. denticulatus and ?C. parvulus are nevertheless consistent enough to suggest that these species do not belong to the same genus Xanthodius Stimpson, 1859 as previously supposed. Also we presume that ?C. parvulus does not belong to the genus Cataleptodius.
Sun, Xi; Wang, Li-Fu; Feng, Ying; Xie, Hui; Zheng, Xiao-Ying; He, Ai; Karim, Md Robiul; Lv, Zhi-Yue; Wu, Zhong-Dao
Canthariasis is a disease of humans caused by the infestation of beetle larvae. It is the second important insectal disease after myiasis. Several species of beetles are reported to cause the disease in gastrointestinal tract, urogenital system, nasal sinuses, ears and faces of mammals. The cigarette beetle Lasioderma serricorne is a widespread and destructive pest that usually feeds on tobacco, tea, beans, cereal grains, and animal and plant specimen. While there was no previous evidence of human infestation by this worm, we report the first case of L. serricorne infestation in a baby girl in China. Here the case, an eight-month-old baby girl with irritable feeling, rubbing eyes, history of contact with mud and eating oranges twice during five days before attendance, and having "worms" in her stool was admitted to the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. The clinical examination revealed that the pulse rate, blood pressure and temperature were regular, and the examination of the head, neck, and chest were unremarkable. The stool specimens containing "worms" were sent to the Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University. The worms were recovered, studied morphologically using naked eyes and anatomical lens, PCR analyzed targeting cytochrome oxidase subunit 1 (COX1) and 18S rRNA genes, examined by sequence analyses of the PCR products and finally classified by phylogenetic analysis to identify their species. Based on the findings, the worms were diagnosed as the larvae of L. serricorne. This report implies that the baby had an infestation with the larvae of L. serricorne in the gastrointestine. During contact with mud or eating oranges by the girl, worm eggs were swallowed into the stomach and resisted gastric acid digestion which eventually hatched into larvae and caused canthariasis. The 8 months girl had underdeveloped immune system which might facilitate the disease. This report implicates that L. serricorne can infest human accidentally and cause canthariasis that may lead to severe damage to infant and older patient upon involvement of important organs of the body. The patients once diagnosed having canthariasis should be treated in time.
Ntiba, M. J.; Harding, D.
The type and quantity of food, the feeding intensity as well as the diel feeding pattern of the long rough dab Hippoglossoides platessoides are described. The interaction of the species with the biological environment is also assessed. The North Sea long rough dab feeds throughout the year with a definite maximum from April till August when over 85% of the fish sampled were feeding. It feeds principally on crustaceans (natant decapods), polychaetes, and echinoderms (ophiuroids), but also consumes O-group whitting ( Merlangius merlangus), long rough dab, and gobies (Gobiidae) in the east-central North Sea nursery grounds during the summer months. The species does not complete strongly for food with the other three most abundant pleuronectids, viz. plaice ( Pleuronectes platessa), common dab ( Limanda limanda) and lemon sole ( Microstomus kitt), as shown by a comparison of major prey items in their stomachs and the anatomical structure of their digestive tracts. The differences in the diet between the North Sea population(s) of long rough dab and the population(s) of the species from other North Atlantic areas are probably a manifestation of ecological differences amongst these ecosystems, especially in the availability and the distribution of prey organisms.
Chaudhari, Aparna; Gireesh-Babu, P; Tripathi, Gayatri; Sabnis, Supriya; Dhamotharan, K; Vardarajan, Remya; Kumari, Kavita; Dasgupta, Subrata; Rajendran, K V
The decapod crustacean Penaeus monodon survives large fluctuations in salinity through osmoregulation in which Na+/K(+)-ATPase (NKA) activity in the gills plays a central role. Adult P. monodon specimens were gradually acclimatized to 5, 25 and 35 per thousand salinities and maintained for 20 days to observe long-term alterations in NKA expression. Specific NKA activity assayed in gill tissues was found to be 3 folds higher at 5 per thousand compared to 25 per thousand (isosmotic salinity) and 0.48 folds lower at 35 per thousand. The enzyme was immunolocalized in gills using mouse α-5 monoclonal antibody that cross reacts with P. monodon NKA α-subunit. At 5 per thousand the immunopositive cells were distributed on lamellar tips and basal lamellar epithelium of the secondary gill filaments and their number was visibly higher. At both 25 per thousand and 35 per thousand NKA positive cells were observed in the inter-lamellar region but the expression was more pronounced at 25 per thousand. Gill architecture was normal at all salinities. However, the 1.5 fold increase in NKA α-subunit mRNA at 5 per thousand measured by quantitative RT-PCR (qRT-PCR) using EF1α as reference gene was not statistically significant. The study confirms the osmoregulating ability of P. monodon like other crustaceans at lower salinities. It is likely that significant increase in NKA transcript level happens at an earlier time point. At higher salinities all three methods record only marginal or no change from isosmotic controls confirming the hypothesis that the animal largely osmoconforms in hyperosmotic environment.
Carramaschi, Isabel Nogueira; de Andrade Pereira, Lorrane; Dos Santos Baia, Vitor; Dos Santos Mallet, Jacenir Reis; de Carvalho Queiroz, Margareth Maria; Zahner, Viviane
The biocidal activity of three strains of Brevibacillus laterosporus upon the post-embryonic developmental stages of Chrysomya megacephala was evaluated. Bioassays were performed to verify lethal and sub-lethal effects including ultra-structural changes in the midgut. Among the strains assayed, Shi3 presented the highest larval mortality rates, achieving 70% at a concentration of 1×10(8) spores/g of diet. Transmission electron microscopy revealed intestinal alterations caused by all strains tested. The findings of this study indicate that Shi3 represents a promising tool for use in the biocontrol of C. megacephala. Copyright © 2017. Published by Elsevier Inc.
Denys Yemshanov; Frank H. Koch; Daniel W. McKenney; Marla C. Downing; Frank Sapio
Nonindigenous species have caused significant impacts to North American forests despite past and present international phytosanitary efforts. Though broadly acknowledged, the risks of pest invasions are difficult to quantify as they involve interactions between many factors that operate across a range of spatial and temporal scales: the transmission of invading...
The relaxation of trade restrictions in the 1960s and 70s led to an unintended exchange of invasive insect species as well as manufactured goods between the United States and its new trade partners. Consequently, the number of exotic insect pests accidently entering and taking up residence in the U...
Reddy, P.S. ); Bhagyalakshmi, A. )
Environmental contamination by metals has increased in recent years due to the excessive use of metals in agriculture and industry. Due to their bioconcentration, immutable and non-degradable properties, these metals constitute a major source of pollutants. Among these metals cadmium, lead and mercury are non-essential, where as copper, iron, manganese, and zinc are essential elements. They are required in trace amounts by all forms of life but are toxic when present in excess. Considerable information is available on the toxic effects of cadmium on biological mechanisms at all integration levels, such as molecular, biochemical, physiological and behavioural, in animals. It is also well known that heavy metal contamination alters cellular physiology, particularly by affecting aspects such as transport across plasma membranes, mitochondrial functions, lysosomal stability etc. Even though it has been demonstrated that the in vitro addition of heavy metals stimulates membrane lipid peroxidation, the in vivo effects exerted by different cations on this process are still not clear. The present work reports the effect of exposure to sublethal concentrations of heavy metals such as Cu and Cd on lipid peroxidation in the tissues of the edible freshwater crab, Oziotelphusa senex senex. 16 refs., 3 tabs.
Pérez-Barros, Patricia; Calcagno, Javier A.; Lovrich, Gustavo A.
Munida gregaria and M. subrugosa have been considered two different species for more than a century; however, after a recent molecular phylogenetic study, they are considered a single polymorphic species. Yet, the use of markers to diagnose species may be misleading when divergence between species is recent, since a speciation event may be obscured by the retention and stochastic sorting of ancestral polymorphisms. The morphs gregaria and subrugosa of Munida gregaria constitute an interesting case for the study of behavioural isolation since they are sympatric, breed at the same time of the year, and might have experienced a recent speciation. Mating behaviour observations and mate choice mating trials were conducted in order to investigate the potential existence of a behavioural prezygotic barrier to gene flow between these two morphs. Since factors involved in mate choice in galatheids are unknown, the four possible combinations of the two different morphs in trios were used to test for the existence of mate choice. Video recordings of all the possible trio combinations revealed that there was cross-attraction between males and females of different morphs. Females bearing partial broods participated in encounters as well as non-ovigerous females. The frequency and duration of homo- and heterotypic encounters were registered, and a reproductive isolation index was calculated for each variable for each trio. The isolation indexes calculated were not different from zero indicating random mating, and were not affected by the composition of the trio or the partial ovigerous condition of females. These results provided evidence of the absence of behavioural prezygotic barriers to gene flow between the morphs gregaria and subrugosa of M. gregaria.
Dynamics of cell and tissue genesis in the male reproductive system of ticks (Acari: Ixodidae) Amblyomma cajennense [corrected] (Fabricius, 1787) and Amblyomma aureolatum (Pallas, 1772): a comparative analysis.
Sampieri, Bruno Rodrigues; Labruna, Marcelo Bahia; Bueno, Odair Correa; Camargo-Mathias, Maria Izabel
Ticks are classified into three families: Argasidae, Ixodidae, and Nutalliellidae. The taxonomy and phylogeny within Ixodidae are still discussed by the specialists, thus requiring further studies. Amblyomma cajennese and Amblyomma aureolatum (Brazil) belong to two species complexes known as "cajennese" and "ovale", respectively, and are directly related to the transmission of the Brazilian spotted fever. This confirms the medical and veterinary significance of these species, as well as the need for further morphological studies that will bring a better understanding of their taxonomy, phylogeny, and control. In this context, the present study aimed to characterize the morphology of the male reproductive system of A. cajennese and A. aureolatum when unfed and after 4 days of feeding, thereby seeking to: (a) distinguish the two species or "complexes", and (b) study an internal system which has the potential to be targeted by acaricides. Therefore, males from both species (unfed and after 4 days of feeding) were cold-anesthetized, dissected, and had their reproductive systems removed for histological analysis. The results showed that the morphology of the male reproductive system is generally similar between both species, like in other Ixodidae ticks, exhibiting a multilobed accessory gland complex related to seminal fluid secretion, a pair of vasa deferentia and a pair of testes housing germ cells (spermatocytes) in different stages. The main differences were found in the development of the accessory gland complex cells and germ cells, showing that the maturation of the male reproductive system starts later in A. aureolatum, when compared to A. cajennese. However, during the blood meal, A. aureolatum development is increased, thus making germ cell maturation and gland complex activity higher than in A. cajennese. This study shows the differences in the development of the male reproductive systems of both species, while providing information that can assist in the establishment of new control methods.
Notes on the Reproductive Ecology and Description of the Preimaginal Morphology of Elaphrus sugai Nakane, the Most Endangered Species of Elaphrus Fabricius (Coleoptera: Carabidae) Ground Beetle Worldwide.
Elucidating the basic life-history of endangered species is the first important step in the conservation of such species. This study examined the reproductive ecology and the preimaginal morphology of the endangered ground beetle Elaphrus sugai Nakane (Coleoptera: Carabidae); currently, the Watarase wetland of the central Kanto Plain, Japan is the only confirmed locality of this beetle species. Laboratory rearing of reproductive adults collected in early April revealed that females can lay more than 131 eggs. Eggs were laid in mud, without an egg chamber. Larvae reached adulthood when fed a diet of mealworms, indicating that E. sugai larvae are insect larvae feeders. An earthworm diet, the optimal diet for larvae of a congeneric species (E. punctatus Motschulsky), was lethal to E. sugai larvae. The egg stage was 3-4 days in duration under a 16L8D cycle (22°C). The duration from hatching to adult eclosion was 23-42 days at various temperatures simulating those of the reproductive period. Larval morphology was similar to that of consubgeneric species described previously. The pupa is unusual, in that the setae on the abdominal tergites are long (twice as long as those of the abdominal segment) and have somewhat "coiled" apices. Finally, the current endangered status of E. sugai was compared to that of E. viridis Horn, which has been regarded as the most endangered species of the genus worldwide.
Mahlmann, Thiago; de Oliveira, Favízia Freitas
We describe a new species of the bee genus Centris, Centris (Centris) byrsonimae Mahlmann & Oliveira sp. n., whose name has appeared as a nomen nudum in the literature since 1985. Further, a new species group of Centris s.str. is proposed, the pulchra group, based on morphological characters, which comprises the species Centris pulchra Moure, Oliveira & Viana, 2003 and Centris byrsonimaesp. n..Based on information from specimen labels studied and data from the literature, a list of plant species visited by the pulchra group is presented. The male genitalia and hidden metasomal sterna 7 and 8 of Centris pulchra are described for the first time. Typographic errors pertaining to the paratype labels reported in the original description of Centris pulchra are corrected. One female paratype of Centris pulchra is designated herein as a paratype of Centris byrsonimaesp. n. An updated list of species of Centris s.str. from northeastern Brazil is provided including references about geographic distributions as well as an identification key to the pulchra species group.
Fancello, Luca; Cillo, Davide; Bazzato, Erika
Cephalocteus scarabaeoides is recorded from the south-western coast of Sardinia, in sandy habitat (marine dunes near the beach), for the first time. The species and the subfamily are new for the Italian fauna.
Pan, Zhao; Bologna, Marco A
The nominate subgenus of the mylabrine genus Mylabris is revised: five new species, M. (M.) alpicola sp.n., M. (M.) cernyi sp.n., M. (M.) mediorientalis sp.n., and M. (M.) pseudoemiliae sp.n., are described and figured; M. (M.) apiceguttata sp.n., is provisionally refered to the nominate subgenus. M. (M.) rishwani Makhan, 2012 is synonymized with M. (M.) quadripunctata (Linnaeus, 1767). The other 20 species are characterized by short descriptions and figures, and a key to the species is provided. Tentatively, M. barezensis and M. batnensis are placed in the nominate subgenus. The bionomics of the species is summarized in tables including information on phenology, elevation, habitat preference, host plants, larval biology, and host insects. Zoogeographic analysis of the subgenus was carried out on the basis of all available faunistic records from literature and collections which are summarized in Appendix.
Mao, Aping; Zhou, Jing; Bin Mao; Zheng, Ya; Wang, Yufeng; Li, Daiqin; Wang, Pan; Liu, Kaiyu; Wang, Xiaoping; Ai, Hui
Pheromone-binding proteins (PBPs) are essential for the filtering, binding and transporting of sex pheromones across sensillum lymph to membrane-associated pheromone receptors of moths. In this study, three novel PBP genes were expressed in Escherichia coli to examine their involvement in the sex pheromone perception of Maruca vitrata. Fluorescence binding experiments indicated that MvitPBP1-3 had strong binding affinities with four sex pheromones. Moreover, molecular docking results demonstrated that six amino acid residues of three MvitPBPs were involved in the binding of the sex pheromones. These results suggested that MvitPBP1-3 might play critical roles in the perception of female sex pheromones. Additionally, the binding capacity of MvitPBP3 with the host-plant floral volatiles was high and was similar to that of MvitGOBP2. Furthermore, sequence alignment and docking analysis showed that both MvitGOBP2 and MvitPBP3 possessed an identical key binding site (arginine, R130/R140) and a similar protein pocket structure around the binding cavity. Therefore, we hypothesized that MvitPBP3 and MvitGOBP2 might have synergistic roles in binding different volatile ligands. In combination, the use of synthetic sex pheromones and floral volatiles from host-plant may be used in the exploration for more efficient monitoring and integrated management strategies for the legume pod borer in the field. PMID:27698435
Holecová, Milada; Maryańska-Nadachowska, Anna; Rozek, Maria
The structure of the karyotypes of two Otiorhynchus species belonging to separate subgenera, viz. Otiorhynchus s.str. bisulcatus and O. (Zadrehus) atroapterus, is compared and described for the first time. Both species have the same chromosome number (2n = 22), sex chromosome system of an achiasmate parachute type (Xy(p)), symmetric karyotype with the prevalence of metacentrics, similar meiotic behaviour, localization of NORs and positive DAPI signals. The main differences involve the morphology of autosomes and the X chromosome in the C-banding pattern and DAPI/CMA3 signals as well as in the presence of additional B chromosomes.
Steullet, P; Guerin, P M
Gas chromatography-coupled electrophysiological recordings (GC-EL) from olfactory sensilla within the capsule of Haller's organ of the tick Amblyomma variegatum indicate the presence of a number of stimulants in rabbit and bovine odours, and in steer skin wash. Some of these stimulants were fully identified by gas chromatography-mass spectrometry analysis and by matching electrophysiological activity of synthetic analogues as: 1) hexanal, 2-heptenal, nonanal, furfural, benzaldehyde, and 2-hydroxybenzaldehyde (in all extracts); 2) heptanal, 2-, 3-, and 4-methylbenzaldehyde, and gamma-valerolactone (only in bovine and rabbit odour). Careful examination of the electrophysiological responses permit characterization of 6 receptor types: 1) a benzaldehyde receptor, 2) a 2-hydroxybenzaldehyde receptor, 3) three types of receptors responding differently to aliphatic aldehydes, and 4) a lactone receptor.
Dutta, S; Biswas, S; Mukherjee, K; Chakrabarty, U; Mallik, A; Mandal, N
White spot disease (WSD) caused by white spot syndrome virus (WSSV) creates severe epizootics in shrimp aquaculture industry worldwide. Despite several efforts, no such permanent remedy was yet developed. Selective breeding using DNA markers would be a cost-effective strategy for long-term solution of this problem. In the present investigation, out of 30 random primers, only one primer produced a statistically significant (P < 0.01) randomly amplified polymorphic DNA (RAPD) marker of 502 bp, which provided a good discrimination between disease resistant and disease susceptible populations of Penaeus monodon from three geographical locations along the East coast of India. Because RAPD markers are dominant, a sequence characterized amplified region (SCAR) marker was developed by cloning and sequencing of 502 bp RAPD fragment, which generates a single 457 bp DNA fragment after PCR amplification only in the disease resistant shrimps. Challenge experiment was also conducted to validate this 457 bp SCAR marker, and the results suggested that the WSSV loads were 2.25 × 10(3) fold higher in disease susceptible than that in disease resistant shrimps using real-time PCR. Therefore, this 457 bp DNA SCAR marker will be very valuable towards the development of disease-free shrimp aquaculture industry. © 2013 John Wiley & Sons Ltd.
A new shallow-water squat lobster, Galathea ryuguu, is described on the basis of material obtained from a colony of unidentified sea fan of the genus Muricella Verrill, 1868. The new species is most closely allied to G. squamea Baba, 1979, but is distinguished by the ornamentation and armature of the carapace, third maxilliped, and ambulatory legs.
Anholeto, Luís Adriano; Oliveira, Patrícia Rosa de; Rodrigues, Rodney Alexandre Ferreira; Spindola, Caroline Dos Santos; Labruna, Marcelo Bahia; Pizano, Marcos Aparecido; Castro, Karina Neoob de Carvaldo; Camargo-Mathias, Maria Izabel
The use of synthetic acaricides is currently the main method to control ticks. However, the indiscriminate use of these chemicals can lead to the selection of resistant individuals and in the accumulation of chemical residues in the environment, contaminating the soil and water streams, consequently affecting the flora, fauna, and the human beings as well. In this sense, the objective of this study was to investigate the acaricidal effect of crude ethanolic extract of Acmella oleracea (L.) R.K. Jansen aerials parts at different concentrations on fed males and semi-engorged females of A. cajennense s.s. An in vitro bioassay (Adult Immersion Test) was carried out to determine the lethal concentration 50 (LC50) of ethanolic extract, calculated by Probit analysis. The results showed that the fed males were sensitive to all the concentrations of A. oleracea ethanolic extract, and mortality rate progressively increased (15-65%) in higher ethanolic extract concentrations. However, semi-engorged females were not sensitive to all the concentrations used here. In the highest concentration (100mg/mL), a mortality rate of 100% was observed after 72h of exposure, indicating that the acaricidal effect would probably be dose-dependent. The LC50 values obtained for the fed A. cajennense s.s males and semi-engorged females were 29.4534mg/mL (limits: 24.4467-41.3847mg/mL) and LC50=17.6335mg/mL (limits: 5.2506-23.5335mg/mL), respectively. Copyright Â© 2016 Elsevier GmbH. All rights reserved.
Baia, Tainá Costa; Campos, Alessandra; Wanderley, Bruno Mattos Silva; Gama, Renata Antonaci
This study investigated the potential effects of flunitrazepam (known as "date rape drug") on the developmental cycle of Chrysomya megacephala, an important forensic species, and their possible implications for the calculation of the PMI. A 1050 C. megacephala eggs were divided into five groups with seven replications each. The eggs were placed on artificial diet prepared with four drug concentrations of flunitrazepam (4, 8, 16, and 32 ng/g), besides the control group (prepared with water). Were evaluated the potential effects on development time, weight gain, and mortality during the cycles. The drug had no significant effect on development time or mortality although it did affect the weight of the pupae and adults (Kruskal-Wallis, p < 0.05). The result can be deduced that the determination of the postmortem interval is not affected.
This study investigated the effects of the anaesthetic, enflurane, on metabolic rates and ventilation patterns in the spotted sugar ant, Camponotus maculatus, using flow-through respirometry. The standard metabolic rate was not affected by the anaesthetic. While the ants were anaesthetised they exhibited a similar discontinuous gas exchange cycle to that observed when they were voluntarily motionless, but their spiracles remained open for a longer time during the open or burst phase even though the amount of CO(2) emitted during this phase remained constant. We discuss this finding in the context of the central nervous system control of the spiracle muscle. For both the determination of standard metabolic rate and ventilation patterns the individual ant has to be motionless. From this study we recommend the use of enflurane to ensure immobility in ants, and other small active insects, during the determination of standard metabolic rates, but the anaesthetic cannot be used to quantify the respiration pattern.
Goodwyn, P J Perez; Voigt, D; Fujisaki, K
We examined the morphology of setae and microtrichia in Aquarius paludum during larval development using a scanning electron microscope. We then conducted immersion experiments with larvae and adults in oxygenated and deoxygenated water. The adult water strider body is covered by a pilose double layer consisting of upper long setae (30-80 microm) and lower filiform microtrichia (5-9 microm). Only setae are present on the legs. Microtrichia on the larval body are very short: 0.5-0.6 microm in first and second instars, and 0.8-1.7 microm in third to fifth instars. Larval body setae are approximately as long as those of adults (25-50 microm), but are much less dense at 1,800-5,750 setae per mm(2) versus 15,000-20,000 setae per mm(2) in adults. The density of setae on the legs remains relatively constant throughout development (larvae: 15,000-20,000 setae per mm(2); adults: 20,000-26,000 setae per mm(2)). Immersion experiments demonstrated that young instars may use cuticular respiration. First- and second-instar larvae survived underwater for several hours without a visible air supply, although they did not survive in deoxygenated water. We posit that the short body microtrichia have a waterproofing function in larvae, whereas they create a compressible air bubble in adults. In adults, waterproofing is accomplished by the setae. The density and length of setae on the legs of larvae was nearly the same as that on the body and legs of adults and is presumably optimized for waterproofing. Thus, a change in morphometrical parameters can result in a large functional change in the same structure. We discuss this interpretation in both ecological and physiological contexts. 2008 Wiley-Liss, Inc
Jose, S; Jayesh, P; Sudheer, N S; Poulose, G; Mohandas, A; Philip, R; Singh, I S Bright
Shrimp cell lines are yet to be reported and this restricts the prospects of investigating the associated viral pathogens, especially white spot syndrome virus (WSSV). In this context, development of primary cell cultures from lymphoid organs was standardized. Poly-l-lysine-coated culture vessels enhanced growth of lymphoid cells, while the application of vertebrate growth factors did not, except insulin-like growth factor-1 (IGF-1). Susceptibility of the lymphoid cells to WSSV was confirmed by immunofluoresence assay using monoclonal antibody against the 28 kDa envelope protein of WSSV. Expression of viral and immune-related genes in WSSV-infected lymphoid cultures could be demonstrated by RT-PCR. This emphasizes the utility of lymphoid primary cell culture as a platform for research in virus-cell interaction, virus morphogenesis, up and downregulation of shrimp immune-related genes, and also for the discovery of novel drugs to combat WSSV in shrimp culture.
Jeyasankar, Alagarmalai; Premalatha, Selvaraj; Elumalai, Kuppusamy
Objective To evaluate the antifeedant, insecticidal and growth inhibition activities of Solanum pseudocapsicum (S. pseudocapsicum) seed extracts against Spodoptera litura (S. litura) and Helicoverpa armigera (H. armigera). Methods Hexane, diethyl ether, dichloromethane and ethyl acetate seed extracts were prepared and tested for antifeedant, insecticidal and growth inhibitory activities against fourth instar larvae of S. litura and H. armigera. Results Ethyl acetate extract showed promising antifeedant and insecticidal activities against S. litura and H. armigera. Percentage of deformed larvae, pupae and adults were maximum in treatment of ethyl acetate extract. Percentage of successful adult emergence was deteriorated by seeds on extract treated larvae. Conclusions Ethyl acetate extracts of S. pseudocapsicum, showed higher efficiency of antifeedant, insecticidal and growth inhibition activities. Hence, it can be used to controll agricultural insect pests, S. litura and H. armigera. PMID:23593579
Hu, Meiying; Chen, Shaohua; Muhammad, Rizwan-ul-Haq; Dong, Xiaolin; Gong, Liang
Deregulated reactive oxygen species (ROS) production can lead to the disruption of structural and functional integrity of cells as a consequence of reactive interaction between ROS and various biological components. Catalase (CAT) is a common enzyme existing in nearly all organisms exposed to oxygen, which decomposes harmful hydrogen peroxide, into water and oxygen. In this study, the full length sequence that encodes CAT-like protein from Spodoptera litura named siltCAT (GenBank accession number: JQ_663444) was cloned and characterized. Amino acid sequence alignment showed siltCAT shared relatively high conservation with other insect, especially the conserved residues which defined heme and NADPH orientation. Expression pattern analysis showed that siltCAT mRNA was mainly expressed in the fat body, midgut, cuticle and malpighian tube, and as well as over last instar larvae, pupa and adult stages. RNA interference was used to silence CAT gene in SL-1 cells and the fourth-instar stage of S. litura larvae respectively. Our results provided evidence that CAT knockdown induced ROS generation, cell cycle arrest and apoptosis in SL-1 cells. It also confirmed the decrease in survival rate because of increased ROS production in experimental groups injected with double-stranded RNA of CAT (dsCAT). This study implied that ROS scavenging by CAT is important for S. litura survival. PMID:23555693
Mysore, Keshava; Shyamala, Baragur V; Rodrigues, Veronica
The antennal lobes of different castes of the ant species Camponotus compressus show a marked diversity in the organization of their olfactory glomeruli. Notably, there is a significant difference in the number and size of glomeruli between the reproductives and the workers and among the different worker castes. In this report, we investigate the notion that these caste-specific differences in glomerular number might be accounted for, at least in part, by the differences in numbers of olfactory sensilla that target the antennal lobe. For this, we examine the number of sensilla on the antennal flagella of all the individual castes of C. compressus. This analysis reveals a striking correlation between sensillar number and the number of antennal glomeruli in a given caste. As a first step in investigating the causal mechanisms that might give raise to this correlation, we carry out an initial characterization of olfactory system development in the minor workers of C. compressus. We analyze the temporal pattern of innervations of the developing antennal lobe by olfactory sensory neuron axons. We document the development of the olfactory glomeruli in the antennal lobe during this process, which occurs during early pupal stages. Our findings provide the basis for future manipulative developmental studies on the role of sensory afferent number in glomerular development of different castes within the same species.
Notes on the Reproductive Ecology and Description of the Preimaginal Morphology of Elaphrus sugai Nakane, the Most Endangered Species of Elaphrus Fabricius (Coleoptera: Carabidae) Ground Beetle Worldwide
Elucidating the basic life-history of endangered species is the first important step in the conservation of such species. This study examined the reproductive ecology and the preimaginal morphology of the endangered ground beetle Elaphrus sugai Nakane (Coleoptera: Carabidae); currently, the Watarase wetland of the central Kanto Plain, Japan is the only confirmed locality of this beetle species. Laboratory rearing of reproductive adults collected in early April revealed that females can lay more than 131 eggs. Eggs were laid in mud, without an egg chamber. Larvae reached adulthood when fed a diet of mealworms, indicating that E. sugai larvae are insect larvae feeders. An earthworm diet, the optimal diet for larvae of a congeneric species (E. punctatus Motschulsky), was lethal to E. sugai larvae. The egg stage was 3–4 days in duration under a 16L8D cycle (22°C). The duration from hatching to adult eclosion was 23–42 days at various temperatures simulating those of the reproductive period. Larval morphology was similar to that of consubgeneric species described previously. The pupa is unusual, in that the setae on the abdominal tergites are long (twice as long as those of the abdominal segment) and have somewhat “coiled” apices. Finally, the current endangered status of E. sugai was compared to that of E. viridis Horn, which has been regarded as the most endangered species of the genus worldwide. PMID:27415755
Tantulo, Uras; Fotedar, Ravi
An 11-day trial was conducted to investigate the osmoregulatory capacity (OC) and regulation of K(+), Na(+), Ca(2+) and Mg(2+) of Penaeus monodon juveniles when exposed to K(+) deficient inland saline water (ISW) of four different salinities (5, 15, 25 and 35 ppt). The survival of juveniles showed a positive linear relationship (R(2) ranging from 0.72 to 0.98) with salinity. At the end of the trial, juveniles were able to survive only in 5 ppt of ISW and showed no changes in OC when transferred from ocean water (OW) to ISW. Further, the OC of juveniles in 5 ppt of ISW was significantly different (P<0.05) from the OC of juveniles exposed to 15, 25 and 35 ppt and exhibited strong serum K(+), Na(+), Ca(2+) and Mg(2+) regulation monitored over 16 h. In contrast, at 35 ppt, significant decrease (P<0.05) in serum K(+) and Mg(2+) concentrations and accumulation of serum Na(+) concentration occurred after 16 h of exposure to ISW. At higher salinity, an increase in serum Na(+) concentration leads to an increase in the serum osmolality of the juveniles, which in turn causes decrease in the OC of the juveniles. The results of this study suggest that K(+) deficiency in ISW has a negative effect on survival, OC and the ability of P. monodon juveniles to regulate serum Na(+), K(+), Ca(2+) and Mg(2+) concentrations. These effects are compounded as salinity increases.
First report of toxicity of Xylopiaparviflora (A. Rich.) Benth (Annonaceae) root bark's essential oil against cowpea seed bruchid, Callososbruchus maculatus Fabricius (Coleoptera: Chrysomelidae: Bruchinae).
Babarinde, Samuel Adelani; Pitan, Olufemi Olutoyin Richard; Olatunde, Ganiyu Olatunji; Ajala, Michael Oluwole
The fumigant toxicity of Xylopia parviflora (A. Rich.) Benth (Annonaceae) root bark's essential oil (EO) against cowpea seed bruchid, Callosobruchus maculatus, was investigated in the laboratory. Dose had significant (P < 0.0001) effect on mortality at 6 hours after treatment (HAT) at a concentration of 6.25 μL/mL air which exerted 81.70% mortality, while there was no mortality in all other lower doses. At 12 HAT, 75.05% and 90.00% mortality were observed at doses of 3.15 and 6.25 μL/mL air, respectively. It was significantly (P < 0.05) higher than the mortality (50.58%) observed when 0.78 μL/mL air was applied. The lethal time for 50% of assayed adults (LT50) obtained when the bruchid was exposed to X. parviflora EO at a dose of 6.25 μL/mL air (2.71 h) was significantly lower than LT50 obtained at exposure of bruchid to other lower doses of 0.78-3.15 μL/mL air.
Mitochondrial COI and morphological evidence for host specificity of the black cherry aphids Myzus cerasi (Fabricius, 1775) collected from different cherry tree species in Europe (Hemiptera, Aphididae)
Rakauskas, Rimantas; Havelka, Jekaterina; Zaremba, Audrius; Bernotienė, Rasa
Abstract Partial sequences of the mitochondrial COI gene of forty eight European and two Turkish population samples of Myzus cerasi from different winter hosts (Prunus spp.) were subjected to phylogenetic analyses. The analysed M. cerasi samples emerged as paraphyletic relative to a Myzus borealis sample used as an out-group, and formed two major clades in neighbor joining, maximum parsimony, maximum likelihood and Bayesian inference trees, corresponding to subspecies living specifically on Prunus avium and P. cerasus. Multivariate discriminant analysis (method of canonical variates) was applied to find out if morphological variation of samples correlated with mitochondrial COI and host plant information. Mean scores on the first two canonical variables clustered samples fully in accordance with their COI haplotypes and host plants confirming the existence of two morphologically similar winter host - specific subspecies of M. cerasi in Europe. No single morphological character enabled satisfactory discrimination between apterous viviparous females of the two subspecies. A three-character linear discriminant function enabled 92.37% correct identification of apterous viviparous females of M. cerasi cerasi (n = 118) and 93.64% of M. cerasi pruniavium (n = 110). A key for the morphological identification of the two subspecies is presented and their taxonomic status is discussed. PMID:24715766
Kaya, Murat; Bağrıaçık, Nil; Seyyar, Osman; Baran, Talat
There has been no study on the chitin structure of wasp species. Here, we selected the three most common wasp species belonging to the family Vespidae for chitin extraction and characterization. Chitin was isolated from each wasp species and characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), elemental analysis (EA), and scanning electron microscopy (SEM). The chitin contents of Vespa crabro, Vespa orientalis, and Vespula germanica were 8.3, 6.4, and 11.9%, respectively. The crystalline index (CrI) values for the chitin extracted from each species were 69.88, 53.92, and 50%, respectively. The most important finding of the study is that although the same method was used to extract chitin from each of the three wasp species, the degree of acetylation was different: for V. crabro and V. orientalis it was 96.85 and 99.82% (the chitin was extremely pure), respectively, whereas that for V. germanica the chitin was 79.83%. © 2015 Wiley Periodicals, Inc.
Screwworm, Cochliomyia hominivorax (Coquerel) Diptera: Calliphoridae), is a devastating pest of all warm blooded animals. Successful eradication of the screwworm has been achieved from continental North America. Maintaining a barrier against reinfestation by screwworms requires rapid, accurate ident...
Mass production, sterilization and release of screwworms (Cochliomyia hominivorax (Coquerel)) that were competitive in the field significantly contributed to the successful application of the sterile insect technique for eradication of screwworms from continental North America. Metabolic byproducts...
The availability of complete mitochondrial genome data for Diptera, one of the largest Metazoan orders, in public databases is limited. Herein, we generated the complete or nearly complete mitochondrial genomes for Cochliomyia hominivorax, Haematobia irritans, Phormia regina and Sarcophaga crassipa...
Screwworms, Cochliomyia hominivorax (Coquerel) (Calliphoridae: Chrysomyinae), are devastating pests of warm blooded animals that cause significant economic damage to livestock. The successful campaign to eradicate screwworms from continental North America using the sterile insect technique, led by t...
Liancalus Loew, 1857 and Scellus Loew, 1857 (Insecta, Diptera, DOLICHOPODIDAE): Proposed conservation of the names by designation of Dolichopus regius Fabricius, 1805 as type species for Anoplomerus Rondani, 1856 [Case 3681
Justin B. Runyon; Michael A. Ivie; Neal L. Evenhuis
The purpose of this application, under Article 81.1 of the Code, is to conserve the genus-group names Liancalus Loew, 1857 and Scellus Loew, 1857 (Diptera, DOLICHOPODIDAE), widely distributed and well-established genera of dolichopodid flies whose names have been used consistently for more than one-and-ahalf centuries. They are threatened by a change in the type...
Girish, B. P.; Swetha, CH.; Reddy, P. Sreenivasula
The objective of the present study was to investigate the expression profile of retinoid X receptor ( RXR), ecdysone receptor ( EcR) and ecdysone inducible gene ( E75) in the hepatopancreas and ovary of Oziothelphusa senex senex during different vitellogenic stages. RXR, EcR and E75 complementary DNAs (cDNAs) were isolated from the ovaries, while vitellogenin ( VtG) cDNA was isolated from the hepatopancreas of vitellogenic female crab. Deduced amino acid sequence of the messenger RNAs (mRNAs) of RXR, EcR and E75 showed more than 80 % identity with their respective mRNAs of other brachyurans. VtG mRNA was not detected in the ovary throughout vitellogenic stages. RXR and EcR were significantly increased in the ovaries during vitellogenic stage I. The levels of EcR, E75 and VtG in the hepatopancreas elevated significantly during vitellogenic stages I and II, whereas the levels of RXR elevated only in vitellogenic stage I. During vitellogenic stage III, the levels of RXR, EcR and VtG in the hepatopancreas were significantly decreased. Immunoprecipitation analysis revealed the presence of VtG in the haemolymph, hepatopancreas and ovary extracts from the females but absent in haemolymph and hepatopancreas extract of males. It can be inferred that RXR, EcR and E75 are involved in the regulation of synthesis of VtG in hepatopancreas, whereas in ovary, it is hypothesized that they play an important role in the uptake of VtG from the haemolymph, probably by regulating the levels of vitellogenin receptor. These are the first data showing an association between the expression levels of RXR, EcR and E75 and vitellogenesis and provide an alternative molecular intervention mechanism to the traditional eyestalk ablation to induce vitellogenesis and ovarian maturation in crustaceans.
Larval development of the pedunculate barnacles Octolasmis angulata Aurivillius 1894 and Octolasmis cor Aurivillius 1892 (Cirripedia: Thoracica: Poecilasmatidae) from the gills of the mud crab, Scylla tranquebarica Fabricius, 1798.
Yap, F C; Wong, W L; Maule, A G; Brennan, G P; Lim, L H S
Detailed studies of larval development of Octolasmis angulata and Octolasmis cor are pivotal in understanding the larval morphological evolution as well as enhancing the functional ecology. Six planktotrophic naupliar stages and one non-feeding cyprid stage are documented in details for the first time for the two species of Octolasmis. Morphologically, the larvae of O. angulata and O. cor are similar in body size, setation patterns on the naupliar appendages, labrum, dorsal setae-pores, frontal horns, cyprid carapace, fronto-lateral gland pores, and lattice organs. Numbers of peculiarities were observed on the gnathobases of the antennae and mandible throughout the naupliar life-cycle. The setation pattern on the naupliar appendages are classified based on the segmentation on the naupliar appendages. The nauplius VI of both species undergoes a conspicuous change before metamorphosis into cyprid stage. The cyprid structures begin to form and modify beneath the naupliar body towards the end of stage VI. This study emphasises the importance of the pedunculate barnacle larval developmental studies not only to comprehend the larval morphological evolution but also to fill in the gaps in understanding the modification of the naupliar structures to adapt into the cyprid life-style.
Komai, Tomoyuki; Saito, Yuma; Myorin, Eiji
A new species of hermit crab of the family Paguridae, Pagurus rectidactylus, is described and illustrated on the basis of material from inshore waters in the Seto Inland Sea and the Sea of Japan. It appears closest to P. quinquelineatus Komai, 2003, also known from Japan, but is easily distinguished from the latter by the dactylus of the right cheliped almost unarmed or armed only with a few tiny spines or tubercles, instead of dorsal and dorsomesial rows of conspicuous spines, and the more elongate and slender ambulatory legs with more numerous ventral corneous spines on dactyli and propodi. Housing is also different between the two species: the new species exclusively inhabits carcinoecia formed by a hydrozoan Stylactaria misakiensis (Iwasa, 1934), whereas P. quinquelineatus uses gastropod shells without association with coelenterates. A checklist of East Asian (Japanese Archipelago to Taiwan Island, Far Eastern Russia to northern China) species of Pagurus along with general geographical distributions is presented.
TRANSGENIC CRYLC* GENE ROUGH RICE LINE TIC-19 DOES NOT CHANGE THE HOST PREFERENCES OF THE NON-TARGET STORED PRODUCT PEST, RHYZOPERTHA DOMINICA (FABRICIUS) AND ITS PARASITOID WASP, ANISOPTEROMALUS CALANDRAE
Rough rice grains are often stored for extended periods before they are used or consumed. However, during storage, the rough rice is vulnerable to insect infestation, resulting in significant economic loss. Previous studies have shown that volatile cues, physical characteristics, and taste chemicals...
Król, Nina; Kiewra, Dorota; Lonc, Elżbieta; Janaczyk, Bartłomiej; Chodorowska-Skubiszewska, Anna; Dzięcioł, Michał; Gola, Mateusz; Gruszka, Robert; Jackowska-Szlachcic, Ewa; Jagiełło, Magdalena; Kawski, Szczepan; Łukaszewski, Zbigniew; Mizgalski, Piotr; Narajowska, Tatiana; Niedzielska, Justyna; Noczyński, Marcin; Rudkowska, Małgorzata; Rzepka, Dariusz; Samulska, Katarzyna; Senze, Michał; Sieczko, Piotr; Silny, Arkadiusz; Staniewska, Anna; Stańczyk, Janusz; Stańczyk, Wojciech; Stasiak, Magdalena; Włodarczyk, Marek; Zimniak, Szymon
Tests performed in 2013 and 2014 revealed the occurrence of three tick species parasitizing pet cats and dogs in the Wrocław Agglomeration. In total, 1,455 tick specimens were removed from 931 hosts (760 dogs and 171 cats) in 18 veterinary clinics. The dominant tick species was Ixodes ricinus (n=1272; 87.4%), followed by I. hexagonus (n=137; 9.4%) and Dermacentor reticulatus (n=46; 3.2%). Females were the most often collected development stage among I. ricinus and D. reticulatus, and nymphs among I. hexagonus. Additionally, D. reticulatus ticks (n=337) were then collected from vegetation in the Wrocław area to detect Babesia canis; however, none was found positive. Only 9.0% of dog blood samples sent to VETLAB were positive for Babesia spp. Negative results for B. canis from ticks may result from the short period of the occurrence of D. reticulatus in the Wrocław area and therefore the vectorpathogen cycle may not have been fully established at the time of the study. Nevertheless, D. reticulatus is expanding its range, and the size of its population in the Wrocław Agglomeration is increasing. The presence of the pathogenic Babesia spp. combined with the occurrence of its main vector¸ D. reticulatus, suggests that the epizootiological situation in the area can change and may pose a new veterinary problem in the future. Dermacentor reticulatus, Babesia canis, pets, Wrocław, Poland.
Dvořák, Libor; Beuk, Paul LT
Abstract Background Anisopodidae (window gnats or wood gnats) is a small family of nematocerous Diptera. Until now only Sylvicola (Anisopus) punctatus (Fabricius, 1787) and Sylvicola (Sylvicola) fenestralis (Scopoli, 1763) were reported from Romania. New information New faunistic records of Sylvicola (Diptera: Anisopodidae) are presented. Sylvicola (Sylvicola) cinctus (Fabricius, 1787) and S. (Anisopus) fuscatus (Fabricius, 1775) are recorded from Romania for the first time. An identification key and illustrations of Romanian Sylvicola species are presented. PMID:26929721
... horticola) Turnip moth (Agrotis segetum) American bollworm moth (Helicoverpa armigera) Cabbage webworm moth (Hellula undalis, Fabricius) The cabbage moth (Mamestra brassicae) Oriental leafworm moth...
Baldin, Edson L L; Fujihara, Ricardo T; Boiça, Arlindo L; Almeida, Maria C de
We describe the new association of Hexacladia smithii (Ashmead) parasitizing two passion fruit bugs, Holhymenia histrio (Fabricius) and Anisoscelis foliacea marginella (Dallas) (Hemiptera: Coreidae), in Brazil.
Guarding against the introduction of screwworms, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), to North America or any other screwworm free area relies on rapid, reliable identification of suspected cases. Identification of first instars suspected to be C. hominivorax can be rapidly v...
Gravid screwworm flies, Cochliomyia hominivorax, are attracted to the volatiles from waste larval rearing media to deposit eggs. Studies were conducted to identify chemicals from the waste media volatiles and determine their effectiveness to attract gravid flies to oviposit. Volatiles were collected...
The waste artificial larval rearing media of the New World Screwworm, Cochliomyia hominivorax (Coquerel) were evaluated to determine their effectiveness as oviposition attractants. Various concentrations of waste larval media resulting from rearing screwworm larvae in gel and cellulose fiber-based ...
The current artificial diet for mass rearing screwworm, Cochliomyia hominivorax, (Coquerel) (Diptera: Calliphoridae) larvae is a semi-solid medium consisting of dry whole bovine blood, poultry egg powder and a milk substitute mixed with a bulking and solidifying agent and water. To reduce the mass r...
The screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), was a devastating pest to all warm blooded animals in the United States and the rest of North America. Successful eradication of the screwworm was achieved by using the unique approach called the sterile insect technique. He...
Screwworm fly, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), in its larval stage is a parasite of warm-blooded animals. Screwworm has been successfully eradicated from the United Sates and Central America using the sterile insect technique. Here we present how the field observations o...
Bovine blood inoculated with bacteria isolated from screwworm-infested animal wounds was tested against gravid screwworm flies, Cochliomyia hominivorax (Coquerel) in the laboratory in a cage bioassay as an attractant for oviposition. Eight species of gram-negative coliform (Enterobacteriaceae) bacte...
Screwworms, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), were devastating pests in parts of North America and Central America before their eradication by means of the sterile insect technique (SIT). Now, a barrier is maintained to prevent re-entry of screwworms from endemic regions t...
Screwworms, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), were devastating pests to all warm blooded animals in the United States and the rest of North America. Successful eradication of screwworms was achieved by using the sterile insect technique. Here we discussed the development a...
Spray-dried whole bovine blood, dry poultry egg, and a dry milk substitute are the constituents of the standard artificial diet currently used for mass rearing screwworm larvae, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). Due to high cost and uncertainty of the commercial supply of ...
Screwworms, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), were devastating pests to all warm blooded animals in the United States and the rest of North America. Successful eradication of screwworms was achieved by using the unique approach called the sterile insect technique. Here we ...
Screwworms, Cochliomyia hominivorax (Coquerel), are devastating pests of warm blooded animals. They have been eradicated from continental North America using the sterile insect technique (SIT). Proper implementation of SIT is an example of the requirements of area-wide control of insect pests. Area-...
New World screwworms (NWS), Cochliomyia hominivorax (Coquerel), are one of the most important arthropod pests of livestock in the Western Hemisphere. Early instars are very difficult to distinguish morphologically from several closely related blow fly species. Random amplified polymorphic DNA polyme...
The sterile insect technique is currently used by the Comisión Panamá - Estados Unidos para la Erradicación y Prevención del Gusano Barrenador del Ganado (COPEG) to maintain a barrier at the border between Panama and Colombia that prevents screwworms, Cochliomyia hominivorax (Coquerel), from South A...
The New World screwworm (NWS), Cochliomyia hominivorax, is a devastating pest of livestock endemic to sub-tropical and tropical regions of the Western Hemisphere. The larvae feed on the tissue of living animals, including man, and can cause death if untreated. Over 60 years ago the sterile insect te...
The waste product of the artificial larval rearing media of the primary screwworm, Cochliomyia hominivorax, attracts gravid female screwworm flies to oviposit. The volatile component of this waste product was collected using solid phase microextraction techniques and subjected to gas chromatography-...
Background The Primary screwworm, Cochliomyia hominivorax (Coquerel), is a serious pest feeding on living flesh of any warm-blooded animal, including humans. It was eradicated from the United States in the early 1980s using the sterile male technique. However, it was recently detected in populations...
Myiasis caused by screwworms, Cochliomyia hominivorax (Coquerel), is devastating to warm-blooded animals. Application of the sterile insect technique (SIT) has successfully eradicated screwworms from the U.S., Mexico, Central America and some Caribbean Islands. Annual benefits to livestock producers...
Paul W. Schaefer; Gerhard Gries; Regine Gries; Yasutomo Higashiura; Yi-Bin Fan
Our objective was to identify the sex pheromones of two allopatric Lymantria species (Lepidoptera: Lymantriidae): (1) L. serva (Fabricius) in Taiwan whose larvae attack and occasionally defoliate Ficus spp. and (2) L. lucescens (Fabricius) in Honshu, Japan, whose larvae feed on Quercus...
G. P. Markin; D. Larson
The stem mining weevil, Ceutorhynchus litura Fabricius, the gall forming fly, Urophora cardui L., and the seedhead weevil, Larinus planus Fabricius, were established as biological control agents on an 1800 hectare multiple-habitat wildlife refuge in northwestern Oregon in the mid-1990s. At the time, Canada thistle was the most wide spread, aggressive, and difficult...
Pall, José Luis; Coscarón, María del Carmen
Abstract Eight genera and 13 species of the tribe Acanthocerini are recorded in Argentina, i.e., Athaumastus haematicus (Stål), Athaumastus macer Brailovsky, Athaumastus subcarinatus (Stål), Athaumastus subterlineatus Bergroth, Beutelspacoris sanchezi Brailovsky, Beutelspacoris dilatata Casini, Camptischium clavipes (Fabricius), Crinocerus sanctus (Fabricius), Dersagrena flaviventris (Berg), Dersagrena lacerdae (Signoret), Dersagrena subfoveolata (Berg), Thlastocoris hernandezi Brailovsky and Zoreva dentipes Fabricius. Redescriptions are given for Athaumastus haematicus, Athaumastus subcarinatus and Dersagrena flaviventris with photographs of male and female genitalia of Dersagrena subfoveolata. Zoreva recorded from Argentina the first time. New locality records are given for Buenos Aires, Chaco, Formosa, Misiones, and Tucumán. PMID:23794912
LaCourse, Sylvia M.; Martinez, Raquel M.; Spach, David H.; Fang, Ferric C.
Cochliomyia hominivorax, the New World screwworm, was a serious livestock pest in the southern United States until the 1960s, when it was successfully eradicated by the release of sterile male flies. It remains endemic in parts of the Caribbean and South America, and there is concern that climate change may extend its geographic distribution. Cochliomyia hominivorax is voracious and can cause extensive damage to soft tissue and bone. We describe the case of a 26-year-old traveler who presented with otalgia and bloody otorrhea after returning from a vacation in the Dominican Republic, where exposure to screwworm flies most likely occurred during a nap on the beach. The causative agent was recognized by its characteristic larval anatomy, which includes pigmented dorsal tracheal trunks and posterior spiracles with an open peritreme. PMID:25510727
LaCourse, Sylvia M; Martinez, Raquel M; Spach, David H; Fang, Ferric C
Cochliomyia hominivorax, the New World screwworm, was a serious livestock pest in the southern United States until the 1960s, when it was successfully eradicated by the release of sterile male flies. It remains endemic in parts of the Caribbean and South America, and there is concern that climate change may extend its geographic distribution. Cochliomyia hominivorax is voracious and can cause extensive damage to soft tissue and bone. We describe the case of a 26-year-old traveler who presented with otalgia and bloody otorrhea after returning from a vacation in the Dominican Republic, where exposure to screwworm flies most likely occurred during a nap on the beach. The causative agent was recognized by its characteristic larval anatomy, which includes pigmented dorsal tracheal trunks and posterior spiracles with an open peritreme. © The American Society of Tropical Medicine and Hygiene.
Transgenic cry1C(⁎) gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae).
Sun, Xiao; Yan, Miao-Jun; Zhang, Aijun; Wang, Man-Qun
Rough rice grains are often stored for extended periods before they are used or consumed. However, during storage, the rough rice is vulnerable to insect infestation, resulting in significant economic loss. Previous studies have shown that volatiles cues, physical characteristics, and taste chemicals on the grains could be the important key behavior factors for storage insect pests to locate the hosts and select oviposition sites. It is also well known that the transgenic Bt rough rice line T1C-19, which expresses a cry1C(⁎) gene has a high resistance to Lepidoptera pests. However, there were no evidences to show the consequences of host preference for non-target insect pests after growing Bt transgenic rice. In this study, the potential key factors of Bt rough rice were investigated for their impacts on the behaviors of non-target pest lesser grain borer Rhyzopertha dominica, the main weevil pest of grain and its parasitic wasps Anisopteromalus calandrae, the natural enemy of the beetle. Both electronic nose and electronic tongue analyses showed that the parameters of Bt rough rice were analogous to those of the non-Bt rough rice. The volatile profiles of Bt and non-Bt rough rice examined by gas chromatographic mass spectrometry (GC-MS) were similar. For most volatile compounds, there were no significantly quantitative differences in compound quantities between Bt and non-Bt rough rice. The densities of sclereids and trichomes on the rough rice husk surface were statistically equal in Bt and non-Bt rough rice. The non-target pest, R. dominica, and its parasitoid wasp, A. calandrae, were attracted to both rough rice and could not distinguish the transgenic T1C-19 from the isogenic rough rice. These results demonstrated that Bt rough rice has no negative impacts on the host preference behaviors of non-target stored product pest R. dominica and its parasitoid A. calandrae.
López-García, Margarita M; Gasca-Álvarez, Héctor J; Cave, Ronald D; Amat-García, Germán
An updated and annotated checklist of the Pentodontini (Coleoptera: Scarabaeidae: Dynastinae) of the New World is presented. The tribe is composed of 32 genera and 151 species, including the introduced species Heteronychus arator (Fabricius).
Members of the Miridae (Lygus lineolaris Palisot de Beauvois and Pseudatomoscelis seriatus Reuter) and Pentatomidae (Acrosternum hilare Say, Euschistus servus (Say), E. tristigmus (Say), E. quadrator Rolston, Oebalus pugnax (Fabricius), Piezodorus guildinii (Westwood), and Thyanta custator accerra M...
Male antennae of Phyllophaga tristis (Fabricius) (Coleoptera: Scarabaeidae: Melolonthinae) were tested using a coupled gas chromatograph-electroantennogram detector (GC-EAD) system for electrophysiological responses to five sex pheromones identified from other Phyllophaga species including L-valine ...
Five records of Pipunculidae (Diptera) attacking proconiine sharpshooters (Auchenorrhyncha: Cicadellidae) are documented here for the first time. Eudorylas alternatus (Cresson) is documented as a parasitoid of Cuerna obtusa Oman and Beamer, and Oncometopia orbona (Fabricius) is recorded as being at...
Little is known about the roles of sex pheromones in mate-finding behavior of social wasps (Vespidae). Working with the aerial yellowjacket, Dolichovespula arenaria (Fabricius), baldfaced hornet, D. maculata (L.), western yellowjacket, Vespula pensylvanica (Saussure), southern yellowjacket, V. squam...
Two species of dynastine scarab beetles are reported for the first time on the island of Hawaii: the Pasadena masked chafer, Cyclocephala pasadenae (Casey)(Scarabaeidae: Dynastinae: Cyclocephalini) and the Temnorhynchus retusus (Fabricius)(Scarabaeidae: Dynastinae: Pentodontini). The Pasadena mask...
Coscarón, María Del Carmen; Pall, José Luis
Eight genera and 21 species of the tribe Anisoscelini (Coreidae, Coreinae) are recorded in Argentina: Anisoscelis foliaceus (Fabricius); Coribergia declivicollis (Berg); Dalmatomammurius vandoesburgi (Brailovsky); Holymenia hystrio (Fabricius); Leptoglossus chilensis (Spinola); L. cinctus (Herrich-Schaeffer); L. concolor Walker; L. crassicornis (Dallas); L. dentatus Berg; L. fasciatus (Westwood); L. gonagra (Fabricius); L. impictus (Stål); L. ingens (Mayr); L. neovexillatus Allen; L. quadricollis (Westwood); L. stigma (Herbst); L. vexillatus (Stål); L. zonatus (Dallas); Phthia lunata (Fabricius); Phthiacnemia picta (Drury) and Ugnius kermesinus (Linnaeus). A key to genera belonging to the tribe is provided. L. stigma is recorded for the first time in Argentina with new locality records for La Rioja, Salta and San Juan.
Strongygaster triangulifera (Loew) (Diptera:Tachinidae) is reported for the first time as a parasitoid of Megacopta cribraria (Fabricius) (Heteroptera: Plataspidae), the kudzu bug, an introduced pest of soybeans and other legume crops in the southeastern U.S....
Vargas, Héctor A; Hausmann, Axel
Three species of geometrid moths are reported from the extreme North of Chile. All three are new for the Chilean fauna: Scopula umbilicata (Fabricius, 1794) (Sterrhinae), Cataspilates grisescens Warren, 1897, and Pero obtusaria Prout, 1928 (Ennominae).
Two new species of Methiini (Tessaropa hispaniolae Lingafelter, Methia dolichoptera) (Coleoptera: Cerambycidae) from Hispaniola are diagnosed, described, and illustrated. The Dominican Republic represents a New Country Record for Malacopterus tenellus (Fabricius) (Oemini), and all hispaniolan local...
Nardi, Gianluca; Mifsud, David
Abstract The Bostrichidae of the Maltese Islands are reviewed. Ten species are recorded with certainty from this Archipelago, of which 6 namely, Trogoxylon impressum (Comolli, 1837), Amphicerus bimaculatus (A.G. Olivier, 1790), Heterobostrychus aequalis (Waterhouse, 1884), Sinoxylon unidentatum (Fabricius, 1801), Xyloperthella picea (A.G. Olivier, 1790) and Apate monachus Fabricius, 1775 are recorded for the first time. Two of the mentioned species (Heterobostrychus aequalis and Sinoxylon unidentatum) are alien and recorded only on the basis of single captures and the possible establishment of these species is discussed. Earlier records of Scobicia pustulata (Fabricius, 1801) from Malta are incorrect and should be attributed to Scobicia chevrieri (A. Villa & J.B. Villa, 1835). A zoogeographical analysis and an updated checklist of the 12 species of Bostrichidae recorded from the Maltese Islands and neigbouring Sicilian islands (Pantelleria, Linosa and Lampedusa) are also provided. Rhizopertha dominica (Fabricius, 1792) form granulipennis Lesne in Beeson & Bhatia, 1937 from Uttarakhand (northern India) was overlooked by almost all subsequent authors. Its history is summarized and the following new synonymy is established: Rhizopertha dominica (Fabricius, 1792) form granulipennis Lesne in Beeson & Bhatia, 1937 = Rhyzopertha dominica (Fabricius, 1792), syn. n. Finally, records of Amphicerus bimaculatus from Azerbaijan, of Bostrichus capucinus (Linnaeus, 1758) from Jordan and Syria, of Scobicia chevrieri from Jordan and Italy, of Xyloperthella picea from Italy, and of Apate monachus from Corsica (France) and Italy, are also provided. PMID:25685033
Fischer, G; Fisher, B L
The myrmicine genus Pheidole Westwood is revised for the smaller islands of the Southwest Indian Ocean: Comoros, Juan de Nova Island, Mauritius, Mayotte, Reunion, and the Seychelles. Descriptions and keys are provided for the thirteen species on these islands of which seven are newly described: P. decepticon sp. n., P. dodo sp. n., P. komori sp. n, P. loki sp. n., P. megatron sp. n., P. ragnax sp. n., P. vulcan sp. n.; and six were previously described: P. braueri Forel, P. fervens Smith, F., P. jonas Forel, P. megacephala (Fabricius), P. parva Forel, and P. teneriffana Forel. New synonymies (with the senior synonym listed first) include P. parva Mayr = P. flavens var. farquharensis Forel, P. parva Mayr = P. tarda Donisthorpe, P. megacephala (Fabricius) = P. picata Forel, P. megacephala (Fabricius) = P. punctulata r. gietleni Forel, 1905, P. megacephala (Fabricius) = P. picata var. bernhardae Emery, 1915, P. megacephala (Fabricius) = P megacephala r. scabrior Forel, and P. teneriffana Forel = P. voeltzkowii Forel. Furthermore, lectotypes are designated from the syntypes of P. braueri, P. fervens, P. jonas, P. parva, and P. teneriffana in order to provide a single name-bearing specimen and to facilitate future taxonomic studies. Finally, a neotype is provided for the untraceable or possibly lost type of the cosmopolitan and invasive P. megacephala, which was originally described by Fabricius from Mauritius (the former 'Ile de France').
Mastrangelo, Thiago; Welch, John B.
The New World Screwworm, Cochliomyia hominivorax (Coquerel), is one of the most damaging parasites of livestock, causing millions of dollars in annual losses to producers. The fly is an obligate parasite of warm-blooded animals, including humans. After a successful 50-year eradication campaign, C. hominivorax has been eradicated from the USA, Mexico and Central America by an area-wide integrated pest management approach. Recently, Caribbean and South American countries have expressed an interest in this approach. Aiming to support forthcoming projects in these countries, this review describes the main technical components of past and ongoing AW-IPM campaigns against C. hominivorax. PMID:26466720
Dourmishev, Assen L; Dourmishev, Lyubomir A; Schwartz, Robert A
Ivermectin is a synthetic derivative of the antiparasitic class of compounds known as avermectins. It is a macrolide endectocide with activity against both endoparasites with cutaneous tropism (Strongyloides stercoralis, Ancylostoma braziliense, Cochliomyia hominivorax, Dermatobia hominis, Filaria bancrofti, Wucheria malayi, Onchocerca volvulus, Loa-loa) and ectoparasites such as Sarcoptes scabies, Pediculus humanus, Demodex folliculorum, and Cheyletiella sp. Ivermectin is of great interest in the treatment of patients with different forms of scabies, head lice, demodecidosis, cutaneous larva migrans, cutaneous larva currens, myiasis, and filariasis.
Results of an investigation of the potential application of remote sensing to various fields of public health are presented. Specific topics discussed include: detection of snail habitats in connection with the epidemiology of schistosomiasis; the detection of certain Anopheles breeding sites, and location of transient human populations, both in connection with malaria eradication programs; and detection of overwintering population sites for the primary screwworm (Cochliomyia americana). Emphasis was placed on the determination of ground truth data on the biological, chemical, and physical characteristics of ground waters which would or would not support the growth of significant populations of mosquitoes.
Hatch, Robert Alan
In the short narrative that follows I introduce two new heroes. Although we begin with Fabricius's first sighting in 1596, the new pivot point in the drama is the collaboration between Hevelius and Boulliau that began around 1660. As it happens, Learned Europe paid little attention to Mira in the generation after the first scattered sightings of 1596, indeed, nearly 70 years passed before the New Star was given a working identity. Like Columbus discovering America, Fabricius and Holwarda saw different things - for convenience, I call them Fabricius's Star and Holwarda's Star. Hevelius's Historiola (Danzig, 1662) and Boulliau's Ad astronomos (Paris, 1667) presented a different vision. It made Mira famous. As I shall argue, if Hevelius gave Mira a history, Boulliau gave Mira a future.5 In the end, the New Star not only challenged the ancient cosmos, it became an enduring icon for the New Science, a returning reminder of celestial continuity and cosmic order.
Boner, Patrick J.
In a letter of 27 October 1604, David Fabricius (1564-1617) eagerly reported to Johannes Kepler (1571-1630) his observations of a brilliant new luminary in the constellation of Sagittarius. Fabricius had first observed the new luminary "near the location of the great conjunction,"1 which had occurred just 10 months earlier. His eyes had been drawn to the area by the proximity of the three superior planets when "Mars and Jupiter were conjoined and Saturn had by then returned directly to the location of the great conjunction."2 There, Fabricius had identified "a new star, with no motion of its own," in the outer sphere encasing the cosmos.3 The star had surpassed Jupiter "in diameter and silvery splendor,"4 and its scintillation had proven incomparably swift.
Webster, Reginald P.; LeSage, Laurent; DeMerchant, Ian
Abstract Zeugophora varians Crotch and the family Megalopodidae are newly recorded for New Brunswick, Canada. Twenty-eight species of Chrysomelidae are newly recorded for New Brunswick, including Acalymma gouldi Barber, Altica knabii Blatchley, Altica rosae Woods, Altica woodsi Isely, Bassareus mammifer (Newman), Chrysolina marginata (Linnaeus), Chrysomela laurentia Brown, Crepidodera violacea Melsheimer, Cryptocephalus venustus Fabricius, Neohaemonia melsheimeri (Lacordaire), Neohaemonia nigricornis (Kirby), Pachybrachis bivittatus (Say), Pachybrachis m-nigrum (Melsheimer), Phyllobrotica limbata (Fabricius), Psylliodes affinis (Paykull), Odontota dorsalis (Thunberg), Ophraella communa (LeSage), Ophraella cribrata (LeConte), Ophraella notata (Fabricius), Systena hudsonias (Forster), Tricholochmaea ribicola (Brown), and Tricholochmaea rufosanguinea (Say), which are also newly recorded for the Maritime provinces. Collection data, habitat data, and distribution maps are presented for all these species. PMID:22539900
Loghmani, Amir; Hajiqanbar, Hamidreza; Talebi, Ali Asghar
Pavania setiformis Loghmani & Hajiqanbar sp. nov. (Acari: Heterostigmatina: Dolichocybidae) associated with Onthophagus (Palaeonthophagus) vitulus (Fabricius) (Coleoptera: Scarabaeidae) is described from northeastern Iran. This remarkable new species represents a new setiformis species group characterized by seta-like sc(1), instead of capitate trichobothria. The genus Pavania is thus divided into three species groups: the fusiformis group (15 species), the gymnopleuri group (3 species) and the setiformis group (1 species). We also found P. sabzevarensis Hajiqanbar & Khaustov, 2010 and P. onthophagi Hajiqanbar & Khaustov, 2010 phoretic on Gymnopleurus mopsus (Pallas) and Onthophagus (Euonthophagus) amyntas alces (Fabricius), respectively.
Hou, Zehai; Li, Qinglong; Wei, Cong
Abstract The present investigation provides comparative morphometrics of the final instar nymphs of three dominant cicada species, i.e., Cryptotympana atrata (Fabricius), Meimuna mongolica (Distant) and Platypleura kaempferi (Fabricius), in Guanzhong Plain, China. Particularly, characters on the antennae, legs, and apex of abdomen of both males and females of these three species were investigated and analyzed. In addition, the numbers of hind tibial spines of the final instar nymphs of 21 representatives of Cicadoidea were compared. The results provide useful characteristics for nymph identification of related species and for further taxonomic and phylogenetic analysis of Cicadoidea. PMID:25147447
Kamal J.K. Gandhi; Christopher Asaro; Brittany F. Barnes; Jamie Dinkins; Wood Johnson; Victor C. Mastro; Jim R. Meeker; Daniel R. Miller; John R. Riggins; Kelley E. Zylstra
The Eurasian woodwasp, Sirex noctilio Fabricius (Hymenoptera: Siricidae), is an introduced invasive pest in North America. This siricid woodwasp is native to Europe, Asia, and Africa where it is considered to a secondary colonizer of conifer trees. However, it is a primary colonizer of conifer trees in its non-native zone in the Southern Hemisphere...
Brittany F. Barnes; Daniel R. Miller; Chris Asaro; James R. Meeker; Wood Johnson; Kamal J.K. Gandhi
The Eurasian woodwasp, Sirex noctilio Fabricius (Hymenoptera: Siricidae), is an introduced invasive pest in the Great Lakes region of North America. If S. noctilio is introduced to the southeastern U.S., it may cause severe economic and ecological impacts, especially in the extensive pine plantations.
The objective of this study is to determine the potential effectiveness of aeration in controlling rice weevil, Sitophilus oryzae (L.), and lesser grain borer, Rhyzopertha dominica (Fabricius), as an alternative to the use of insecticides. Grain temperature, moisture, and pest population dynamics we...
Guineafowl of different ages were inoculated intravenously with a H6N2 wild waterfowl-origin low-pathogenicity type A influenza virus. No evidence of clinical disease was observed. The examined infected birds had atrophy of the spleen, thymus, and bursa of Fabricius when compared to the non-infect...
We conducted a field study to determine seasonal egg parasitism rates of the kudzu bug Megacopta cribraria (Fabricius) on the kudzu plant, Pueraria montana (Lour.) Merr. var. lobata (Willd.) Maesen et Almeida ex Sanjappa and Pradeep in Tokyo, Japan during the period from May 2014 to September 2014. ...
Gupta, Ankita; Churi, Paresh V; Sengupta, Ashok; Mhatre, Sarang
In the comprehensive rearing of lepidopterans from peninsular India, eleven species of Lycaenidae were parasitized by ten species of wasps. Four new taxa of lycaenid associated microgastrine wasps Parapanteles eros Gupta n. sp., P. arka Gupta n. sp., P. esha Gupta n. sp., and P. regale Gupta n. sp. reared from Chilades pandava (Horsfield), Curetis thetis (Drury), Prosotas dubiosa (Semper), Tajuria cippus (Fabricius), respectively, are described with diagnostic details and illustrations along with documentation of six species of wasps viz., Apanteles folia, Apanteles sp., Protapanteles sp. 01 & 02 (Braconidae), Charops obtusus obtusus Morley (Ichneumonidae), and Brachymeria lasus (Walker) (Chalcididae). This is the first record of host-parasitoid association of lycaenid butterflies with Parapanteles. Wasps from three different families were recorded: Braconidae, Ichneumonidae, and Chalcididae. The parasitoid species were reared from the following Lycaenidae hosts: Anthene lycaenina (Felder), Arhopala amantes Hewitson, Chilades pandava (Horsfield), Curetis thetis (Drury), Jamides celeno (Cramer), Prosotas dubiosa (Semper), Rathinda amor (Fabricius), Spindasis vulcanus (Fabricius), Tajuria cippus (Fabricius), Tarucus balkanicus nigra Bethune-Baker, and Tarucus callinara Butler. All lycaenids were collected from peninsular India, except T. callinara (central India). A comparative account of all newly described species is provided along with the detailed illustrated description and differences vis-à-vis its closely allied Indian species. Also a comprehensive table comprising parasitoid species, associated host, stage of parasitism, and nature of cocoon is provided.
Carolyn Klass; E. Richard Hoebeke
Sirex wood wasp, (Sirex noctilio Fabricius), was first identified in February 2005 from a Lindgren funnel trap taken September 7, 2004 in Fulton, NY (Oswego County). We followed the development of the events in New York chronologically from this first identification to the results of an expanded trapping program in 2005.
The palmetto weevil, Rhynchophorus cruentatus Fabricius, native to Florida, attacks palm trees. Like its economically destructive relatives, R. ferrugineus (Olivier) and R. palmarum L., it feeds internally and often is not detected until irreparable damage occurs. Acoustic methods previously used su...
A. D. Wilson; N.M. Schiff; D. A. Haugen; E. R. Hoebeke
The wood decay fungus Amylostereum areolatum (Fr.) Boidin, native to Eurasia and North Africa (4), is the mycosymbiont of several siricid woodwasps including Sirex noctilio Fabricius, a major pest of pines in New Zealand, Australia, South America, and South Africa where it has been introduced. Adult females of S. noctilio are effective vectors of...
The present study examines the use of olfactory cues for nest recognition by Megachile rotundata (Fabricius) (Hymenoptera: Megachilidae), an economically important pollinator of seed alfalfa throughout western North America. In-nest observations revealed that nesting females drag their abdomen alon...
The kudzu bug or bean plataspid, Megacopta cribraria (Fabricius), is native to Asia where it appears to be widely distributed (although the taxonomy is not entirely clear), but is infrequently a pest of legumes. This bug appeared in 2009 in the southeastern United States, where it is closely associa...
The sugarcane stem borers Diatraea saccharalis (Fabricius) and D. indigenella Dyar & Heinrich are common pests of sugarcane crops in Colombia’s Cauca river valley (CRV). In 2012, however, D. tabernella Dyar was recorded for the first time in northern CRV and just one year later D. busckella Dyar & H...
This article outlines the fundamental contribution of Max D. Cooper to the analysis of the role of the thymus and of the bursa of Fabricius in the development of immunologic competence both before and after birth, placing a new scientific paradigm in the definition of the ontogeny of the lymphoid tissues.
Kaskaskia Island because of its association with man. It produces a painful bite which is very slow to heal. The black widow spider, Latrodectus ... mactans (Fabricius) also prefers upland forest situations, but may be a possible inhabitant of the island. Two ticks, Dermacentor variabilis (Say), the
Garcete-Barrett, Bolívar R
Stenonartonia tekoraava sp. nov., a new species of potter wasp is described from central Amazonia. By size and color pattern, this species falls into the typically Amazonian mimicry ring of the social wasp Polybia liliacea (Fabricius) [Vespidae: Polistinae]. Comments are made on the Müllerian mimicry rings as a common phenomenon in Hymenoptera and particularly in the family Vespidae.
Bees are among the most important pollinators of flowering plants in most ecosystems. This paper describes a large dataset that represents one of the outcomes of a comprehensive, broadly comparative study on the diversity, biology, biogeography, and evolution of Anthidium Fabricius in the Western He...
Day old broiler chicks were obtained from a commercial hatchery and inoculated either orally or intracloacally with a characterized strain of Campylobacter jejuni. After 1 hr, 1day and 1wk post inoculation, the thymus, spleen, liver/gallbladder, bursa of Fabricius and ceca were aseptically removed ...
Zhou, Yong; Merkl, Ottó; Chen, Bin
Three species of the genus Xenocerogria Merkl, 2007 have been recorded in China, Xenocerogriafeai (Borchmann, 1911), Xenocerogriaignota (Borchmann, 1941) and Xenocerogriaruficollis (Borchmann, 1912). Xenoceraxanthisma Chen, 2002 is proposed as a junior synonym of Xenocerogriaruficollis. Lectotype of Xenocerogriaignota is designated, and the species is transferred to the genus Lagria Fabricius, 1775. New Chinese province records of Xenocerogriaruficollis are provided.
Alfalfa leafcutting bees, Megachile rotundata Fabricius, are excellent pollinators of alfalfa for seed production. Alfalfa leafcutting bee nests consist of a series of cells in a cavity. The female bee lays a single egg in each cell on a pollen-nectar provision. In alfalfa seed production systems...
Maruca vitrata Fabricius is a pantropical lepidopteran pest of legumes. Phylogenetic analysis of a mitochondrial cytochrome c oxidase-I gene (coxI) fragment indicates that three Maruca sp. mitochondrial lineages have unique geographic distributions [lineages 1 and 2: Australia, Taiwan, and West Afr...
Initially discovered in Georgia in 2009, the exotic invasive plataspid, Megacopta cribraria Fabricius has become a serious pest of soybean. Managing M. cribraria in soybean typically involves the application of broad-spectrum insecticides. Soybean host plant resistance is an attractive alternative...
Knowledge of the many mechanisms of vesicular stomatitis virus (VSV) transmission is critical to understanding the epidemiology of sporadic disease outbreaks in the western U.S. Migratory grasshoppers (Melanoplus sanguinipes, Fabricius) have been implicated as reservoirs and mechanical vectors of VS...
Like other ambrosia beetles, Xyleborus volvulus Fabricius (Coleoptera: Curculionidae) lives in a mutualistic symbiotic relationship with fungi that serve as food source. Until recently, X. volvulus was not considered a pest, and none of its symbionts were considered plant pathogens. However, recent ...
Castro-Huertas, Valentina; Schwertner, Cristiano F; Fernández, Fernando
New records of genera and species of stink bugs (Hemiptera: Pentatomidae) from Colombia are provided. Two genera are new records for South America: Alathetus and Schraderiellus. Fifteen genera are new record for Colombia: Agaclitus, Boea, Ceratozygum, Euthyrhynchus, Eritrachys, Doesburguedessa, Lopadusa, Marmessulus, Paralincus, Patanius, Peromatus, Phalaecus, Phoeacia, Rio, and Tyrannocoris. Forty-nine species from five subfamiles are recorded for the first time in Colombia. Asopinae: Coryzorhaphis carneolus Erichson, Coryzorhaphis superba Breddin, Euthyrhynchus floridanus (Linnaeus), Podisus sagitta Fabricius, Stiretrus anchorago (Fabricius), Stiretrus cinctellus Germar, Tylospilus peruvianus Horvath, Tyrannocoris nigriceps Thomas. Cyrtocorinae: Ceratozygum horridum (Germar). Discocephalinae: Agaclitus dromedarius Stål, Antiteuchus melanoleucus (Westwood), Antiteuchus sepulcralis (Fabricius), Dinocoris gibbosus (Fallou), Dinocoris variolosus (Linnaeus), Discocephalessa terminalis (Walker), Dryptocephala crenata Ruckes, Dryptocephala dentifrons (Latreille), Eurystethus ovalis Ruckes, Paralcippus dimidiatus (Ruckes), Alathetus rufitarsus Dallas, Eritrachys bituberculata Ruckes, Paralincus bimaculatus (Ruckes), Schraderiellus cinctus (Ruckes), Xynocoris recavus (Garbelotto & Campos). Edessinae: Brachystethus cribus (Fabricius), Brachystethus tricolor Bolívar, Doesburguedessa elongatispina Fernandes and Lopadusa fuscopunctata (Distant). Pentatominae: Banasa fulgida Thomas, Banasa paraexpallescens Thomas, Dichelops divisus (Walker), Dichelops nigrum Bergroth, Euschistus carbonerus Rolston, Mormidea bovilla (Distant), Mormidea triangularis (Walker), Murgantia bifasciata Herrich-Schaeffer, Murgantia violascens (Westwood), Oebalus pugnax (Fabricius), Oebalus ypsilon-griseus (DeGeer), Odmalea concolor (Walker), Patanius vittatus Rolston, Proxys albopunctulatus (Palisot), Proxys punctulatus (Palisot), Rhyncholepta grandicallosa Bergroth, Rio insularis Ruckes, Roferta
López Millán, Cyntia; Olea, María S; Dantur Juri, María J
Myiasis is caused by dipterous larvae from the Calliphoridae, Sarcophagidae, Muscidae, Cuterebridae, and Syrphidae families. In this work, Cochliomyia hominivorax, Chrysomya megacephala, and Ornidia robusta were identified causing vulva, ear, and leg myiasis in pigs in Tucuman province, northwestern Argentina. The report of the presence of C. hominivorax and C. megacephala is very important due to their role as myiasis-causing and disease vectors. The occurrence of Ornidia robusta is remarkable, since it constitutes the first record of myiasis in general and of myiasis in pigs in particular. Lastly, the presence of Sarcophaga spp. is also interesting, since some of them originate myiasis and are therefore of concern for cattle, wild animals, and human populations.
Prado e Castro, Catarina; Szpila, Krzysztof; Martínez-Sánchez, Anabel; Rego; Silva, Isamberto; Serrano, Artur R.M.; Boieiro, Mário
Abstract Knowledge on the taxonomic diversity and distribution of blowflies from the Madeira Archipelago is updated. New and interesting findings are reported for poorly studied islands and islets of this archipelago, together with a brief analysis of the diversity of Macaronesian Calliphoridae s. l. Seven blowfly species were collected during this study, including the first records of Calliphora vicina Robineau-Desvoidy, 1830, Chrysomya albiceps (Wiedemann, 1819), Lucilia sericata (Meigen, 1826), Pollenia rudis (Fabricius, 1794) and Stomorhina lunata (Fabricius, 1805) from Porto Santo, and of Calliphora vicina, Lucilia sericata and Stomorhina lunata from Desertas Islands. The presence of Calliphora loewi Enderlein, 1903 in Madeira Laurisilva forest is discussed and its first instar larva is redescribed, revealing important differences in relation to its original description. An identification key to the adult Madeiran blowflies is provided for the first time. PMID:27917052
Müller, Günter C; Hogsette, Jerome A; Revay, Edita E; Kravchenko, Vasiliy D; Leshvanov, Andrey; Schlein, Yosef
The horse fly fauna (Diptera: Tabanidae) of Jordan is, after Israel, the richest in the Levant, with 24 known species. During the 20-year project "The Ecology and Zoogeography of the Lepidoptera of the Near East," we regularly collected blood-feeding flies, resulting in 11 additional species of Tabanidae for Jordan. The new records are: Atylotus quadrifarius (Loew, 1874), Chrysops caecutiens (Linnaeus, 1758), Dasyrhamphis nigritus (Fabricius, 1794), Haematopota pallens Loew, 1871, Nemorius irritans (Ricardo, 1901), Philipomyia graeca (Fabricius, 1794), Tabanus cordiger Meigen, 1820, Tabanus taeniola Palisot de Beauvois, 1806, Tabanus quatuornotatus Meigen, 1820, Tabanus separatus Effllatoun, 1930, and Tabanus spectabilis Loew, 1858. Most of the new records (10/11) are of Palearctic origin; of these, six are of a Mediterranean and one each of West Palearctic, Euroasiatic, Irano-Turanian, and Eremic providence. Only one species, T. taeniola, is an Afrotropical-Eremic element. © 2011 The Society for Vector Ecology.
Webster, Reginald P.; Sweeney, Jon D.; DeMerchant, Ian; Silk, Peter J.; Mayo, Peter
Abstract Five species of Cerambycidae, Acmaeops discoideus (Haldeman), Anelaphus villosus (Fabricius), Phymatodes species (CNC sp. n. #1), Sarosesthes fulminans (Fabricius), and Urgleptus signatus (LeConte) are newly recorded for New Brunswick, Canada. All but Acmaeops villosus are new to the Maritime provinces. Phymatodes testaceus (Linnaeus) is removed from the faunal list of the province as a result of mislabeled specimens, records of Phymatodes maculicollis LeConte are presented confirming the presence of this species in New Brunswick, and the first recent records ofNeospondylis upiformis (Mannerheim) are presented. Additional records are given for the recently recorded Phymatodes aereus (Newman), indicating a wider distribution in the province. Collection data, habitat data, and distribution maps are presented for each species. PMID:22539899
Jakhar, K K; Sadana, J R
Feeding of aflatoxin B1 @ 1 ppm to 2-week old Japanese quail for a period of 8 weeks produced gross and microscopic changes in the liver, skeletal muscles, heart and bursa of Fabricius. These included fatty changes, bile duct hyperplasia and lymphoid aggregation in liver; haemorrhages in thigh, breast muscles and myocardium; mild depletion of lymphocytes, cystic degeneration and fibrous tissue proliferation in bursa of Fabricius. More or less similar lesions were seen in quail chicks fed on aflatoxin with sodium selenite @ 5 ppm but these were of lesser intensity and appeared at later stages of the experiment thereby indicating that supplementation of selenium had some protective action against the toxic effect of aflatoxin B1 in Japanese quail.
Berchi, Gavril Marius; Kment, Petr
A critical review of the family Veliidae (Hemiptera: Heteroptera: Gerromorpha) in Romania is provided. In total, two genera and eight species (Microvelia Westwood, 1834-3 species, Velia Latreille, 1804-5 species) are known from the country. Microvelia buenoi Drake, 1920 and Velia serbica Tamanini, 1951 are recorded for the first time from Romania. The occurrence of V. affinis filippii Tamanini, 1947 and V. mancinii mancinii Tamanini, 1947 is confirmed by additional records. Based on proven or suspected misidentifications, V. currens (Fabricius, 1794) and V. rivulorum (Fabricius, 1775) are excluded from the Romanian fauna. A checklist of the Veliidae of Romania and updated distribution maps are provided. Biogeographical aspects of the fauna are summarized.
Tishechkin, Alexey K.; Konstantinov, Alexander S.; Bista, Sanjay; Pemberton, Robert W.; Center, Ted D.
Abstract Criocerine leaf beetles found in Nepal feeding on Dioscorea bulbifera (L.), an invasive weed of Asian origin, were identified as Lilioceris cheni Gressitt and Kimoto based on a synopsis of the Oriental Lilioceris species and review of the Lilioceris impressa species group. All the continental, Oriental species included in the group are diagnosed and illustrated, and a key for their identification is provided. Species status of Lilioceris thibetana Pic, 1916 is resurrected. The following new synonyms are proposed: Lilioceris coomani (Pic, 1928) = Lilioceris egena (Weise, 1922), and Lilioceris subcostata (Pic, 1921a), Lilioceris laticornis (Gressit, 1942), Lilioceris inflaticornis Gressit & Kimoto, 1961, and Lilioceris maai Gressit & Kimoto, 1961 = Lilioceris impressa (Fabricius, 1787). Lectotypes of the following species are designated: Lilioceris coomani Pic, 1928; Lilioceris impressa (Fabricius, 1787); Lilioceris laosensis (Pic, 1916); Lilioceris malabarica (Jacoby, 1904); Lilioceris ruficornis (Pic, 1921b); Lilioceris subcostata (Pic, 1921a); Lilioceris thibetana (Pic, 1916); and Lilioceris unicolor (Hope, 1831). PMID:21747681
Castañeda-Vildózola, Alvaro; Valdez-Carrasco, Jorge; Equihua-Martínez, Armando; González-Hernández, Héctor; Romero-Nápoles, Jesús; Solís-Aguilar, Juan F; Ramírez-Alarcón, Samuel
The male and female genitaliae of three species of the genus Heilipus Germar (H. lauri Boheman, H. pittieri Barber and H. trifasciatus Fabricius) that damage avocado fruits (Persea americana Mill.) in Mexico and Costa Rica are described and illustrated. The aedeagus, spiculum gastrale, styli of 8th sternite are different in each one of the three species studied and can be used for specific identification.
Abdollahi, Tandis; Jalalizand, Ali Reza; Mozaffarian, Fariba; Wilson, Michael
The leafhopper fauna of northwestern Iran: Azarbaijan-e-Sharghi, Azarbaijan-e-Gharbi and Ardabil provinces is listed from previously published records and from our current work. Sixty-nine species are included with four species (Mogangellastraminea Dlabola, 1957, Doraturastylata (Boheman, 1847), Macrostelessordidipennis (Stål, 1858) and Psammotettixseriphidii Emeljanov, 1962) listed as new for Iran and Balcluthapunctata (Fabricius, 1775), as a new record for the region. A distribution map of the species in northwestern Iran is given.
Kehlmaier, Christian; Gharali, Babak; Majnon Jahromi, Bahareh
Corononcodes ziegleri spec. nov. is described and illustrated based on material from Iran. The species represents the second Palaearctic representative of this panopine genus. In addition, C. siculus Bezzi is recorded from Fuerteventura (Canary Islands, Spain) for the first time. An identification key to the Palaearctic species of Corononcodes is presented. Additional faunistic records for the Iranian fauna comprise Acrocera orbiculus (Fabricius) and an apparently undescribed species of Ogcodes Latreille. DNA-barcodes for all Iranian taxa are provided.
Kadej, Marcin; Jaroszewicz, Sylwia
A description of the last larval instar (based on exuviae) of Globicornis corticalis (Eichhoff, 1863) (Coleoptera: Dermestidae) is presented. Morphological characters of Globicornis larvae are characterized and discussed, including antenna, epipharynx, mandible, maxilla, ligula with labial palpi, hastisetae, legs, tergites, and condition of the antecostal suture. Structural differences among mature larvae of G corticalis (Eichhoff, 1863), G emarginata (Gyllenhal, 1808) and G nigripes (Fabricius, 1792) are compared and summarized.
Cassidy, Alan; Rawlins, Andrew
Abstract The taxonomy and distribution of the five species of Hypolycaena in Maluku are discussed and new locality records given. Corrections are made to the published taxonomy and distribution of Hypolycaena phorbas (Fabricius, 1793). This clarification enables a better understanding of the biogeography of the genus. Hypolycaena asahi Okubo, 2007, was originally described from a single female from Ambon and is here recorded from Seram. The male is described for the first time. PMID:21977001
thymus, thymic-related lymph nodes, the bone marrow, the spleen, gut associated lymphoid tissue (GALT) and the chick bursa of Fabricius, S. vrifyina...projections in the day-old chick indicates that the chick, like the mouse, receives vagal innervation during embryonic development (these neuronal centers...represent the source for vagal efferents in the chick ). The intrathymic distribution of these vagal cholinergic (AChE-positive) nerves in the chick
Bijanzad, Peyman; Momayez, Reza; Bozorgmehrifard, Mohammad H; Hablolvarid, Mohammad H; Pourbakhsh, Seyed A
Avian infectious bronchitis virus (IBV) is prevalent in all countries with intensive poultry flocks. This disease is characterised primarily by respiratory signs, but some IBV strains may also infect other organs such as the intestinal and urogenital tracts. The aim of this study was to characterise the histopathological lesions and tissue tropism of Iranian isolate IR/773/2001(793/B) of avian infectious bronchitis virus in different organs of experimentally infected SPF chickens. Forty-two one-day-old, specific pathogen-free (SPF) chicks were divided randomly into two groups (21 chicks to each group). At the age of 12 days, one group was inoculated intra-ocularly with 10(3) EID 50 of the 793/B isolate, and the other was kept as the control group. Tissue samples were collected at 2, 4, 6, 8, 10 and 12 days post-inoculation (PI). The IBV virus was detected in the caecal tonsils and cloaca from the 2nd to the 12th day PI. The virus was also detected in the kidneys from days 4-10 PI and in the bursa of Fabricius from days 4-12 PI. The virus was detected in the trachea, lungs and thymus. The most obvious histopathological lesions were found in the trachea, kidney, lungs and bursa of Fabricius. Amongst the lymphoid tissues, histopathological changes were found most frequently in the bursa of Fabricius. The results of this study indicated that the 793/B serotype of IBV is unlikely to cause mortality, severe clinical signs or gross lesions in infected chickens, but its replication in some tissues including the bursa of Fabricius could render birds susceptible to other micro-organisms.
Nava, Santiago; Beati, Lorenza; Dunlop, Jason; Guglielmone, Alberto A
Herein, Amblyomma tenellumKoch, 1844 (Ixodidae) is reestablished as a valid tick name and removed from the synonymy list of Amblyomma cajennense (Fabricius, 1787), while Amblyomma imitatorKohls, 1958, is relegated to a junior synonym of A. tenellum. Amblyomma tenellum is redescribed based on the examination of male type specimens collected by Deppe at the beginning of the 19th century in Mexico and described by Koch in 1844. Copyright © 2014 Elsevier GmbH. All rights reserved.
Agrain, Federico A; Cabrera, Nora; Holgado, Miriam G; Vicchi, Franco R
Some species of Colaspis Fabricius are well-known pests of several crops in Argentina. In this contribution, we describe a new species within this genus: Colaspis caligula n. sp., found in association with Vitis vinifera (Linnaeus) crops. We provide descriptions and illustrations of the mature larva, pupa and adult, as well as notes on its diagnostic characters, life cycle, and the damages produced to the plants.
The neotropical myrmecophilous genus Smilax is revised and a new species, Smilax deneinephyto Chatzimanolis is described from southern Brazil. Lectotypes are designated for Smilax cyanea Wasmann and Staphylinus pilosus Fabricius. Smilax cyanea is shown to be a junior synonym of Smilax pilosa. A key and illustration of morphological features are provided for the identification of taxa. The types of the two species of Smilax were not available for study even though they are deposited in public museums.
Ye, Xin-hai; van Achterberg, Cornelis; Yue, Qi; Xu, Zai-fu
Abstract The Chinese fauna of the family Leucospidae (Hymenoptera, Chalcidoidea) is reviewed and illustrated for the first time. Twelve species of Leucospis Fabricius, 1775 are recorded; of which two species are new to science: Leucospis aequidentata sp. n. and Leucospis shaanxiensis sp. n. and one species is reported new for China: Leucospis intermedia Illiger, 1807. An identification key to Chinese species is included. A lectotype is designated for Leucospis aurantiaca Shestakov, 1923. PMID:28331388
Webster, Reginald P.; Sweeney, Jon D.; DeMerchant, Ian
Abstract Eleven species of Mordellidae are newly recorded for New Brunswick, Canada. Six of these, Falsomordellistena discolor (Melsheimer), Falsomordellistena pubescens (Fabricius), Mordellistena ornata (Melsheimer), Mordellaria undulata (Melsheimer), Tomoxia inclusa LeConte, and Yakuhananomia bidentata (Say)are new for the Maritime provinces. Falsomordellistena pubescens is new to Canada. Pelecotoma flavipes Melsheimer (family Ripiphoridae) is reported for the first time for New Brunswick and the Maritime provinces. Collection and habitat data are presented for all these species. PMID:22539896
reliable estimate of the macrofaunal community. Calculations were based on the following formula : 3 6- -) N ts) 2i’ Dx • where: s standard deviation of...were determined using the formulae of Folk (1974). The epibenthic community was described from 10 minute trawl * samples taken at the North, South...Benedict Drilonereis app. Eteone heteropoda Hartman Eteone lactea Claparede ~Eeone log (FabriCiUs) Eumida sanguinea (Oersted) Exogene hebes (Webster
Calculations were based upon the following = ° formula : \\Dx ) where: s = standard deviation of the prelimianry sample, t = the tabulated t value at the 0.05...were determined graphically using the formulae of Folk (1974). The epibenthic community was described from 10 minute trawl samples taken at the North...Hartman Eteone lactea Claparede Eteone longa (Fabricius) Eu-mida -sane uinea (Oersted) Exogene he bes (Webster and Benedict) Glycera ameiTcana Leidy
Tan, Jiang-Li; Van Achterberg, Kees; Chen, Xue-Xin
Abstract Twenty two species of the paper wasp genus Ropalidia Guérin-Méneville, 1831, are listed from China. Among them, R. malaisei van der Vecht, 1962, R. cyathiformis (Fabricius, 1804), R. santoshae Das & Gupta, 1989, R. scitula (Bingham, 1897), R. obscura Gusenleitner, 1996 and R. ornaticeps (Cameron, 1900) are new records from China. A new species, R. parartifex Tan & van Achterberg, is described. Their diagnostic characteristics are summarized in an illustrated key and 36 colourplates. PMID:24715777
is to determine whether SDs cause secondary injury after TBI, and therefore represent an acute target for treatment to improve recovery . The...data suggest that therapeutic blockade of SD/PID may be an effective strategy to improve recovery from TBI, and point toward the need for a future...Dreier JP, Major S, Fabricius M. Recovery of slow potentials in AC-coupled electrocorticography by digital inverse filtering: application to spreading
Pereira de Sousa, José Roberto; Carvalho-Filho, Fernando da Silva; Juen, Leandro; Esposito, Maria Cristina
The present study was conducted in five different phytogeographic zones of the Brazilian state of Maranhão, three of which (the Amazon Forest, Cerrado, and Palm Groves) are more heterogeneous, whereas the other two (Marshlands and Mangroves) are more homogeneous. In each zone, nine sites were visited for the collection of necrophagous flies using bait traps in 2010, 2011, and 2012. The calliphorid and sarcophagid communities observed at each site were compared in terms of species richness, composition, and abundance. The more heterogeneous zones had higher species richness, except in the case of the sarcophagids in the forest habitats. The calliphorids Chloroprocta idioidea (Robineau- Desvoidy, 1830), Mesembrinella bicolor (Fabricius, 1805), Hemilucilia semidiaphana (Rondani, 1850) and Lucilia eximia (Wiedemann, 1819) were more closely associated with the Cerrado, Palm Grove and Amazon Forest zones, and Chrysomya megacephala (Fabricius, 194) with the Mangrove. In the sarcophagids, Peckia (Euboettcheria) subducta (Lopes, 1935) and P. (Pattonella) palidipilosa (Curran & Walley, 1934) were associated with the Amazon Forest, and P. (Sarcodexia) lambens (Wiedemann, 1830) and Tricharaea (Sarcophagula) occidua (Fabricius, 1794) with the Palm Grove and Cerrado zones. In the calliphorids, the greatest dissimilarity was recorded between the Amazon Forest and the Mangrove and Lowland grassland zones. In the sarcophagids, by contrast, the greatest dissimilarities were recorded between the Amazon Forest and all the other four zones. In general, then, the phytogeographic zones with the highest environmental heterogeneity were characterized by the greatest species richness and abundance of necrophagous flies. PMID:27798664
Pereira de Sousa, José Roberto; Carvalho-Filho, Fernando da Silva; Juen, Leandro; Esposito, Maria Cristina
The present study was conducted in five different phytogeographic zones of the Brazilian state of Maranhão, three of which (the Amazon Forest, Cerrado, and Palm Groves) are more heterogeneous, whereas the other two (Marshlands and Mangroves) are more homogeneous. In each zone, nine sites were visited for the collection of necrophagous flies using bait traps in 2010, 2011, and 2012. The calliphorid and sarcophagid communities observed at each site were compared in terms of species richness, composition, and abundance. The more heterogeneous zones had higher species richness, except in the case of the sarcophagids in the forest habitats. The calliphorids Chloroprocta idioidea (Robineau- Desvoidy, 1830), Mesembrinella bicolor (Fabricius, 1805), Hemilucilia semidiaphana (Rondani, 1850) and Lucilia eximia (Wiedemann, 1819) were more closely associated with the Cerrado, Palm Grove and Amazon Forest zones, and Chrysomya megacephala (Fabricius, 194) with the Mangrove. In the sarcophagids, Peckia (Euboettcheria) subducta (Lopes, 1935) and P. (Pattonella) palidipilosa (Curran & Walley, 1934) were associated with the Amazon Forest, and P. (Sarcodexia) lambens (Wiedemann, 1830) and Tricharaea (Sarcophagula) occidua (Fabricius, 1794) with the Palm Grove and Cerrado zones. In the calliphorids, the greatest dissimilarity was recorded between the Amazon Forest and the Mangrove and Lowland grassland zones. In the sarcophagids, by contrast, the greatest dissimilarities were recorded between the Amazon Forest and all the other four zones. In general, then, the phytogeographic zones with the highest environmental heterogeneity were characterized by the greatest species richness and abundance of necrophagous flies.
Sreedevi, K; Verghese, Abraham
The aphid, Aphis punicae Passerini (Homoptera : Aphididae) is a serious pest attacking pomegranate (Punica granatum L.), an important semi arid fruit crop grown widely in most parts of the country. The major predators found preying on A. punicae in pomegranate ecosystem were Cheilomenes sexmaculata (Fabricius), Scymnus sp., Pseudaspidemerus circumflexo (Motsch.), Paragus serratus (Fabricius), Ischiodon scutellaris (Fabricius) and Chrysopa sp. The population dynamics and spatial distribution of these predators in an unsprayed pomegranate ecosystem were studied at Indian Institute of Horticultural Research, Bangalore (12 degrees 58' N; 77 degrees 35'E), India during 2000-2002. The predators were found to be distributed uniformly among different tree quadrants and followed the same distributional pattern of A. punicae during their peak in January and February. The predator density was relatively higher in lower canopies than upper canopies. The spatial distribution of predators showed aggregate distribution pattern at higher mean densities and exhibited regular or under-dispersed distribution at lower mean densities. The temporal distribution of aphidophagous predators on A. punicae showed two peaks one during January - February and second during August - September The population of predators started building up along with aphid population and reached maximum at high aphid densities and declined as the prey availability declined. This indicated that predators followed the same trend of their prey, A. punicae, showing a clear numerical response.
Yu, Xiaoxue; Rui, Lei; Shao, Qiang; Liu, Haiwen; Lu, Yanan; Zhang, Yongchao; Li, Zandong
In the current study, we investigate changes in CD4+CD25+ cells in chickens during infectious bursal disease virus (IBDV) infection. The percentage of CD4+CD25+ cells in lymph organs, e.g., the thymus, spleen, bursa of Fabricius and peripheral blood, during the first 1–5 days post infection (dpi) was assessed by flow cytometry. The data revealed a remarkable decrease in the percentage of CD4+CD25+ cells in the thymus from 1 to 5 dpi and in the spleen during early infection. An increase of the percentage of CD4+CD25+ cells among peripheral blood lymphocytes was observed during the first two days of IBDV infection. Additionally, CD4+CD25+ cells infiltrated the bursa along with CD4+ cells after IBDV infection. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the mRNA levels of immune-related cytokines in IBDV-infected thymus and bursa of Fabricius tissues. The data revealed that IBDV caused a significant increase in interleukin (IL)-10 mRNA levels, with the Harbin-1 strain (vvIBDV) inducing higher IL-10 expression than the Ts strain. Taken together, our data suggest that chicken CD4+CD25+ cells may participate in IBDV pathogenicity by migrating from their sites of origin and storage, the thymus and spleen, to the virally targeted bursa of Fabricius during IBDV infection. PMID:25803101
Scultetus, A H; Villavicencio, J L; Rich, N M
Venous valves are delicate structures, the integrity of which is crucial for the normal function of the venous system. Their abnormalities lead to widespread disorders, ranging from chronic venous insufficiency to life-threatening thromboembolic phenomena. The discovery of the venous valves, however, has been the subject of hot controversy. Even though Fabricius ab Aquapendente is credited with the discovery by most historians, we demonstrate in this paper that other anatomists described them many years before Fabricius ab Aquapendente publicly demonstrated them in Padua in 1579. A thorough review of the historical literature surrounding the discovery of the venous valves was carried out from 1545 to the present under the supervision of the Medical History Department of our institution. Research was performed at the History of Medicine Division of the National Library of Medicine and through MEDLINE access to the medical literature. The Parisian Charles Estienne first mentioned the venous valves in his 1545 publication when he described "apophyses membranarum" in the veins of the liver. Lusitanus and Canano publicly demonstrated them in the azygos vein during cadaver dissections performed in Ferrera, Italy. The Parisian Jacques Sylvius described valves in the veins of the extremities in 1555. The work of these anatomists, however, could not achieve full recognition, because Andreas Vesalius, the leading anatomist at that time, was unable to confirm their findings and strongly denied the existence of venous valves. Vesalius's influence was so powerful that research on the subject was idle until 1579, when Fabricius ab Aquapendente "discovered" the venous valves. About the same time, the German Salomon Alberti published the first drawings of a venous valve (in 1585). William Harvey, a disciple of Fabricius ab Aquapendente, finally postulated the function of the venous valves, providing anatomical support for one of the greatest discoveries in medicine: the blood
Hollmen, T.; Franson, J. Christian; Kilpi, Mikael; Docherty, D.E.; Hansen, W.R.; Hario, Martti
Samples of brain, intestine, liver, lung, spleen, and bursa of Fabricius were collected from five common eider (Somateria mollissima) duckling carcasses during a die-off in the western Gulf of Finland (59°50′N, 23°15′E) in June 1996. No viral activity was observed in specific-pathogen-free chicken embryos inoculated with tissue suspensions, but samples of bursa of Fabricius from three birds were positive when inoculated into Muscovy duck (Cairina moschata) embryo fibroblasts. The isolates were characterized as nonenveloped RNA viruses and possessed several characteristics of the genus Orthoreovirus. Virus particles were icosahedral with a mean diameter of 72 nm and were stable at pH 3.0; their genome was separated into 10 segments by polyacrylamide gel electrophoresis. Mallard (Anas platyrhynchos) ducklings experimentally infected with the eider reovirus showed elevated serum activities of aspartate aminotransferase, creatine kinase, and lactate dehydrogenase enzymes and focal hemorrhages in the liver, spleen, and bursa of Fabricius. During 1997–99, the prevalence of neutralizing antibodies to the isolated virus ranged from 0 to 86% in 302 serum samples collected from incubating eider hens at three nesting areas along coastal Finland. The highest seroprevalence was found in Hanko in 1999, just weeks before reports of an uninvestigated mortality event resulting in the death of an estimated 98% of ducklings at that location. These findings raise the question of potential involvement of the virus in poor duckling survival and eider population declines observed in several breeding areas along coastal Finland since the mid-1980s.
Proctor, S.J.; Pearson, G.L.; Leibovitz, L.
The first major epizootic of duck plague in free-flying waterfowl occurred at Lake Andes, South Dakota, in January and February, 1973. Duck plague was diagnosed in black ducks, mallards, pintail-mallard hybrids, redheads, common mergansers, common golden eyes, canvasbacks, American widgeon, wood ducks, and Canada geese, indicating the general susceptibility of ducks to duck plague. Clinical signs observed in mallards were droopiness, polydipsia, lethargy, reduced wariness, weakness, reluctance to fly, swimming in circles, bloody diarrhea, bloody fluid draining from the nares and bill, and terminal convulsions.Because the mallard was the most numerous and heavily infected species during the Lake Andes epizootic, gross and microscopic lesions of the gastrointestinal tract, liver, spleen, thymus, bursa of Fabricius, heart, lung, bone marrow, pancreas, and ovaries were described. Lesions of the esophagus and cloaca were in the stratified submucosal glands. In the small and large intestine, lesions were located in lymphocytic aggregates, lamina propria, and crypt epithelium. Hemorrhages and necrosis of hepatocytes and bile duct epithelium were noted in the liver. Diffuse necrosis of lymphocytic and reticuloendothelial tissue were evident in the spleen, bursa of Fabricius, and thymus. Hemorrhages in other tissues such as the lung and heart were often associated with lymphoid nodules, while those in organs such as the pancreas were associated with acinar necrosis. Intranuclear inclusion bodies were seen in stratified squamous epithelium of the esophagus and cloaca, crypt epithelium of the intestine, hepatocytes, bile duct epithelium, cells of Hassel's corpuscles, splenic periarteriolar reticular cells, and epithelial cells in the bursa of Fabricius.
Han, Deping; Wang, Shuxiang; Hu, Yanxin; Zhang, Yuanyuan; Dong, Xianggui; Yang, Zu; Wang, Jiankui; Li, Junying; Deng, Xuemei
The Silky Fowl (SF) is known for its special phenotypes and atypical distribution of melanocytes among internal organs. Although the genes associated with melanocyte migration have been investigated substantially, there is little information on the postnatal distribution of melanocytes in inner organs and the effect of hyperpigmentation on the development of SF. Here, we analyzed melanocyte distribution in 26 tissues or organs on postnatal day 1 and weeks 2, 3, 4, 6, 10, and 23. Except for the liver, pancreas, pituitary gland, and adrenal gland, melanocytes were distributed throughout the body, primarily around blood vessels. Interaction between melanocytes and the tissue cells was observed, and melanin was transported by filopodia delivery through engulfed and internalized membrane-encapsulated melanosomes. SFs less than 10 weeks old have lower indices of spleen, thymus, and bursa of Fabricius than White Leghorns (WLs). The expression levels of interferon-γ and interlukin-4 genes in the spleen, and serum antibody levels against H5N1 and infectious bursal disease virus were lower in SF than in WL. We also found immune organ developmental difference between Black-boned and non-Black- boned chickens from SFs and WLs hybrid F2 population. However, degeneration of the thymus and bursa of Fabricius occurred later in SF than in WL after sexual maturity. Analysis of apoptotic cells and apoptosis-associated Bax and Bcl-2 proteins indicated that apoptosis is involved in degeneration of the thymus and bursa of Fabricius. Therefore, these results suggest that hyperpigmentation in SF may have a close relationship with immune development in SF, which can provide an important animal model to investigate the roles of melanocyte. PMID:26047316
Abdollahi, Tandis; Jalalizand, Ali Reza; Mozaffarian, Fariba; Wilson, Michael
Abstract The leafhopper fauna of northwestern Iran: Azarbaijan-e-Sharghi, Azarbaijan-e-Gharbi and Ardabil provinces is listed from previously published records and from our current work. Sixty-nine species are included with four species (Mogangella straminea Dlabola, 1957, Doratura stylata (Boheman, 1847), Macrosteles sordidipennis (Stål, 1858) and Psammotettix seriphidii Emeljanov, 1962) listed as new for Iran and Balclutha punctata (Fabricius, 1775), as a new record for the region. A distribution map of the species in northwestern Iran is given. PMID:25931954
Wang, Zhiliang; Alonso-Zarazaga, M. A.; Zhou, Dakang; Zhang, Runzhi
Abstract The preimaginal stages including egg, mature larva and pupa of Pseudaspidapion botanicum Alonso-Zarazaga & Wang, 2011 were described and figured, diagnostic characters of larva and pupa were discussed, and corresponding biological information was supplied. The nomenclature of frontal setae in the larva compared with curculionid weevils, the absence of the hypopharyngeal bracon in the larva, and the metafemoral setae in the pupa were discussed. Common and different characters among the larvae of Pseudaspidapion botanicum, Aspidapion radiolus (Marsham, 1802) and Aspidapion aeneum (Fabricius, 1775) were also provided. PMID:23653504
Wang, Zhiliang; Alonso-Zarazaga, M A; Zhou, Dakang; Zhang, Runzhi
The preimaginal stages including egg, mature larva and pupa of Pseudaspidapion botanicum Alonso-Zarazaga & Wang, 2011 were described and figured, diagnostic characters of larva and pupa were discussed, and corresponding biological information was supplied. The nomenclature of frontal setae in the larva compared with curculionid weevils, the absence of the hypopharyngeal bracon in the larva, and the metafemoral setae in the pupa were discussed. Common and different characters among the larvae of Pseudaspidapion botanicum, Aspidapion radiolus (Marsham, 1802) and Aspidapion aeneum (Fabricius, 1775) were also provided.
Pollock, Darren A.
Abstract The Nearctic fauna (north of Mexico) of Eustrophinae is reviewed, and consists of the following five genera and 12 species: Pseudoholostrophus (Pseudoholostrophus) impressicollis (LeConte), Pseudoholostrophus (Holostrophinus) discolor (Horn), Holostrophus bifasciatus (Say), Eustrophus tomentosus Say, Eustrophopsis confinis (LeConte), Eustrophopsis bicolor (Fabricius), Eustrophopsis brunneimarginatus (Dury), Eustrophopsis indistinctus (LeConte), Eustrophopsis arizonensis (Horn), Eustrophopsis ornatus (Van Dyke), Eustrophopsis crowdyi sp. n., and Synstrophus repandus (Horn). A lectotype is designated for Eustrophus brunneimarginatus Dury. A key is given to separate genera and species, supplemented with illustrations of relevant features, including aedeagi of all Nearctic species of Eustrophopsis. Detailed distribution (including Mexican records) and natural history data are provided. PMID:22611332
Straka, Jakub; Engel, Michael S
The apid cuckoo bees of the Cape Verde Islands (Republic of Cape Verde) are reviewed and five species recognized, representing two genera. The ammobatine genus Chiasmognathus Engel (Nomadinae: Ammobatini), a specialized lineage of cleptoparasites of nomioidine bees is recorded for the first time. Chiasmognathus batelkaisp. n. is distinguished from mainland African and Asian species. The genus Thyreus Panzer (Apinae: Melectini) is represented by four species - Thyreus denoliisp. n., Thyreus batelkaisp. n., Thyreus schwarzisp. n., and Thyreus aistleitnerisp. n. Previous records of Thyreus scutellaris (Fabricius) from the islands were based on misidentifications.
Sáfián, Szabolcs; Pyrcz, Tomasz; Brattström, Oskar
Two new endemic butterfly species from the genus Bebearia: B. oshogbo sp. nov. and B. wojtusiaki sp. nov., are described from western Nigeria; B. oshogbo is most closely related to the Guineo-Congolian B. tentyris (Hewitson) and the Upper Guinean B. osyris (Schultze), whereas B. wojtusiaki constitutes a morphological and biogeographic link between the Central African B. plistonax (Hewitson) and the Upper Guinean endemic B. arcadius (Fabricius). The finding of these new species gives further strong evidence that western Nigeria should be recognized as a distinct biogeographic sub-region of West Africa, as the area hosts a substantial number of endemic taxa (listed in the discussion).
Sharaf, Mostafa R; Aldawood, Abdulrahman S
Ants of the genus Solenopsis Westwood in the Arabian Peninsula are revised. Six species are treated: Solenopsis elhawagryi Sharaf & Aldawood sp. n., S. geminata (Fabricius, 1804), S. omana Collingwood & Agosti, 1996, S. saudiensis Sharaf & Aldawood, 2011, S. sumara Collingwood & Agosti, 1996, and S. zingibara Collingwood & Agosti, 1996. Solenopsis elhawagryi is described from Beljorashi Governorate, Al Baha Province, Saudi Arabia, based on worker castes and the queen with notes on this species biology and ecology. Solenopsis sumara workers are redescribed and illustrated for the first time and a lectotype is designated. An identification key to the Arabian and Egyptian species is provided with scanning electron micrographs to facilitate species recognition.
Webster, Reginald P.; Sweeney, Jon D.; DeMerchant, Ian
Abstract Two species of Geotrupidae, Geotrupes splendidus splendidus (Fabricius) and Odonteus liebecki (Wallis), are newly reported for New Brunswick, Canada. Twelve species of Scarabaeidae are added to the faunal list of the province, including Aegialia criddlei Brown, Caelius humeralis (Brown), Dialytellus dialytoides (Fall), Diapterna omissa (LeConte), Diapterna pinguis (Haldeman), Planolinoides aenictus (Cooper and Gordon), Stenotothorax badipes (Melsheimer), and Ataenius strigatus (Say), which are also newly recorded for the Maritime provinces. Collection data, habitat data, and distribution maps are presented for each species. PMID:22539883
Nerudová, Jana; Kovac, Damir; Tóthová, Andrea
This is the first description of larva and puparium of Oplodontha rubrithorax (Macquart, 1838) from the Oriental Region. Larvae were found at a hot spring in North Thailand. The morphological features and cuticular structures of the larva are documented by drawings and SEM micrographs and the main characters are compared with the European O. viridula (Fabricius, 1775), the only described larva of this genus. Differences between larvae of both species were only found in pubescence. The characteristic, somewhat dilated and slightly clavate hairs on the dorsal surface of the body segments of O. viridula larva are apparently lacking in the larva of O. rubrithorax.
Tsai, S S; Chang, T C; Yang, S F; Chi, Y C; Cher, R S; Chien, M S; Itakura, C
An epornitic of avian pox occurred in rosy-faced lovebirds (Agapomis roseicollis). The infected birds showed a variety of lesions including cutaneous, diphtheritic, systemic and oncogenic entities. Proliferative changes with cytoplasmic inclusion bodies in the cornea, bursa of Fabricius, and cranial and nasal bones which were found in the present cases have not been described previously. Electron microscopic examination of the skin, cornea, and cranial and nasal bones revealed poxvirus virions in the inclusions. Secondary infection of candidiasis was very common in cutaneous pox lesions.
Abstract Background Reduvius iopterus Perty 1834 was first described from the Rio Negro, Brazil. It was then included in Zelus Fabricius 1803 as Z. iopterus (Perty 1834) without critical examination of this action (Stål 1872).The Neotropical genus Aristathlus Bergroth 1913 was described to include two species from the Amazon basin: A. imperatorius Bergroth 1913 and A. regalis Bergroth 1913. New information Based on the original description and illustration of Reduvius iopterus, Aristathlus imperatorius is considered to be its junior synonym, with the resulting new combination: Aristathlus iopterus (Perty 1834). PMID:26175611
Yoshida, Takahiro; Hirowatari, Toshiya
Japanese species of the genus Psammoecus Latreille, 1829 are taxonomically revised. Four new species, P. scitus sp. n. (misidentified with P. quadrimaculatus), P. labyrinthicus sp. n., P. boreas sp. n. and P. omotoensis sp. n. are described. Psammoecus bipunctatus (Fabricius, 1792), P. trimaculatus Motschulsky, 1858 (misidentified with P. triguttatus), P. simoni Grouvelle, 1892, P. fasciatus Reitter, 1874 and P. triguttatus are redescribed. Another described species whose distribution in Japan is questionable. P. quadrimaculatus is also redescribed. Lectotype and paralectotype of P. fasciatus and P. triguttatus are designated.
Libonatti, María Laura; Ruta, Rafał
The Pseudomicrocara Armstrong from Argentina are reviewed. In total, seven species are present: Pseudomicrocara angusta sp. nov., P. antarctica (Fairmaire) comb. nov., P hieroglyphica sp. nov., P. inflexipenis sp. nov., P livida (Fabricius), P. obliquata (Solier) comb. nov., and P patagonica (Curtis) comb. nov. New provincial records are provided for several species. Pseudomicrocara obliquata, previously known only from Chile, is recorded from Argentina for the first time. Illustrations of habitus and genitalia as well as distributional data for all Argentinean species of Pseudomicrocara are provided.
Kazerani, Farzaneh; Khaghaninia, Samad; Talebi, Ali Asghar; Shamshev, Igor
Fourteen species of the genus Empis Linnaeus were collected and identified from Iran. Empis (Lisssempis) guilanensis Kazerani & Shamshev sp. nov. is described and illustrated from Guilan province in north central Iran. The following seven species were recorded from Iran for the first time: E. (Euempis) tessellata Fabricius, 1794; E. (Pachymeria) mediterranea (Loew, 1864); E. (P.) obscuripes (Loew, 1873); E. (Polyblepharis) dedecor Loew, 1869; E. (P.) engeli Chvála, 1999; E. (P.) soror Collin, 1937; E. (P.) spirifera Bezzi, 1909. An identification key to the subgenera and species of Empis from Iran are provided.
Yoshida, Takahiro; Hirowatari, Toshiya
Abstract Japanese species of the genus Psammoecus Latreille, 1829 are taxonomically revised. Four new species, P. scitus sp. n. (misidentified with P. quadrimaculatus), P. labyrinthicus sp. n., P. boreas sp. n. and P. omotoensis sp. n. are described. Psammoecus bipunctatus (Fabricius, 1792), P. trimaculatus Motschulsky, 1858 (misidentified with P. triguttatus), P. simoni Grouvelle, 1892, P. fasciatus Reitter, 1874 and P. triguttatus are redescribed. Another described species whose distribution in Japan is questionable. P. quadrimaculatus is also redescribed. Lectotype and paralectotype of P. fasciatus and P. triguttatus are designated. PMID:24843265
Shahdadi, Adnan; Sari, Alireza; Naderloo, Reza
The present annotated checklist contains 43 species of thoracican barnacles known to date from the area, 33 and 26 from the Persian Gulf and the Gulf of Oman, respectively. Nine species are new records for the area including Amphibalunus subalbidus (Henry, 1973), Armatobalanus allium (Darwin, 1854), Chelonibia patula (Ranzani, 1818), Conchoderma hunteri (Owen, 1830), Lepas anserifera Linnaeus, 1767, Lithotrya valentiana Reinhardt, 1850, Megabalanus coccopoma (Darwin, 1854), Megabalanus occator (Darwin, 1854) and Platylepas hexastylos (Fabricius, 1798), of which A. subalbidus and M. coccopoma are reported as alien species from the region.
Reddy, M.S.; Jayaprada, P.; Rao, K.V.R. )
The increasing contamination of the aquatic environment by the indiscriminate and widespread use of different kinds of pesticides is a serious problem for environmental biologists. Organochlorine insecticides are more hazardous since they are not only more toxic but also leave residues in nature. The deleterious effects of aldrin on several crustaceans have been studied. But studies concerning the impact of aldrin on biochemical aspects of crustaceans are very much limited. The present study is aimed at probing the in vitro effects of aldrin on the acid and alkaline phosphatase activity levels in selected tissues of penaeid prawn, Metapenaeus monoceros (Fabricius).
Liu, L L; He, J H; Xie, H B; Yang, Y S; Li, J C; Zou, Y
This study investigated the effects of dietary resveratrol at 0, 200, 400, or 600 mg/kg of diet on the performance, immune organ growth index, serum parameters, and expression levels of heat shock protein (Hsp) 27, Hsp70, and Hsp90 mRNA in the bursa of Fabricius, thymus, and spleen of 42-d-old female black-boned chickens exposed to heat stress at 37 ± 2°C for 15 d. The results showed that heat stress reduced daily feed intake and BW gain; decreased serum glutathione (GSH), growth hormone, and insulin-like growth factor-1 levels; and inhibited GSH peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities compared with birds subjected to thermo-neutral circumstances. Chickens that were fed diets supplemented with resveratrol exhibited a linear increase in feed intake and BW gain (P < 0.001); serum GSH, growth hormone, and insulin-like growth factor-1 levels (P ≤ 0.01); and GSH-Px, SOD, and CAT activities (P < 0.001) compared with chickens that were fed diets without resveratrol during heat stress. In contrast, serum malonaldehyde concentrations were decreased (P < 0.001) in the chickens fed a resveratrol-supplemented diet. Heat stress also reduced (P < 0.05) the growth index of the bursa of Fabricus and spleen; however, it had no effect on the growth index of the thymus. The growth index of the bursa of Fabricius and spleen increased (P < 0.05) upon heat stress and coincided with an increase in supplemental resveratrol levels. The expression of Hsp27, Hsp70, and Hsp90 mRNA in the bursa of Fabricius and spleen were increased (P < 0.01), but those of Hsp27 and Hsp90 mRNA in thymus were decreased (P < 0.01) under heat stress compared with no heat stress. Resveratrol attenuated the heat stress-induced overexpression of Hsp27, Hsp70, and Hsp90 mRNA in the bursa of Fabricius and spleen and increased the low expression of Hsp27 and Hsp90 mRNA in thymus upon heat stress. The results suggest that supplemental resveratrol improves growth performance
Nemkov, Pavel G
Ten species of Didineis Wesmael 1852 are recorded from Russia and adjacent territories. The new synonymy (valid name listed first) is established for Didineis bactriana Gussakovskij 1937 = D. ogloblini Gussakovskij 1937, D. clavimana Gussakovskij 1937 = D. turanica Gussakovskij 1937, and D. lunicornis (Fabricius 1798) = Didineis ruthenica Gussakovskij 1937. The number of valid species-group taxa in the genus Didineis is reduced to 25. The lectotypes are designated for four species: Didineis bactriana Gussakovskij 1937, D. botsharnikovi Gussakovskij 1937, D. clavimana Gussakovskij 1937, D. ruthenica Gussakovskij 1937. An original key to the species is provided.
Perk, Jacques H. H.
In the first part of this paper I shall discuss the round-about way of how the integrable chiral Potts model was discovered about 30 years ago. As there should be more higher-genus models to be discovered, this might be of interest. In the second part I shall discuss some quantum group aspects, especially issues of odd versus even N related to the Serre relations conjecture in our quantum loop subalgebra paper of 5 years ago and how we can make good use of coproducts, also borrowing ideas of Drinfeld, Jimbo, Deguchi, Fabricius, McCoy and Nishino.
Khaustov, Alexander A
Twenty four species of pygmephoroid mites (Acari: Pygmephoroidea: Neopygmephoridae, Scutacaridae, Microdispidae) are recorded from the ant Lasius flavus (Fabricius) or from its nests from Western Siberia and Crimea. Four of them of the genus Scutacarus Gros, 1845 (Acari: Scutacaridae), S. insolitus sp. nov., S. heterotrichus sp. nov., S. moseri sp. nov. and S. sibiriensis sp. nov. are described as new for science. Four species of scutacarid mites are recorded for the first time in Russia. The comparison of pygmephoroid mite communities associated with Lasius flavus from Crimean and West Siberian populations and notes on phoresy of pygmephoroid mites on ants are provided.
Jarzabek-Müller, Andrea; Morinière, Jérôme; Varandi, Hassan Barimani; Müller, Jörg
Synaptus filiformis Fabricius, 1781 has been recognized as a rather variable elaterid species. Based on morphological distinctness of 3 specimens (2 males, 1 female) from Mazandaran, Iran, a new species is here hypothesised. COI barcoding supports the new species as a new Barcode Index Number with a distance of mean 13.5% to the three other BINs available on the Barcode of Life Database. The new species Synaptus iranicus sp. nov. (BOLD: ACZ9929) and its distinctive features are described. Moreover the results of the DNA barcoding suggest that Synaptus filiformis as yet described is not a single species, but rather a complex of several morphologically similar species.
Sharaf, Mostafa R.; Aldawood, Abdulrahman S.
Ants of the genus Solenopsis Westwood in the Arabian Peninsula are revised. Six species are treated: Solenopsis elhawagryi Sharaf & Aldawood sp. n., S. geminata (Fabricius, 1804), S. omana Collingwood & Agosti, 1996, S. saudiensis Sharaf & Aldawood, 2011, S. sumara Collingwood & Agosti, 1996, and S. zingibara Collingwood & Agosti, 1996. Solenopsis elhawagryi is described from Beljorashi Governorate, Al Baha Province, Saudi Arabia, based on worker castes and the queen with notes on this species biology and ecology. Solenopsis sumara workers are redescribed and illustrated for the first time and a lectotype is designated. An identification key to the Arabian and Egyptian species is provided with scanning electron micrographs to facilitate species recognition. PMID:23226211
Shih, Yi-Jia; Ho, Ping-Ho; Chan, Tin-Yam
Four leucosiid species from Taiwan are presented. Ebalia nudipes Sakai, 1963, with its male first gonopod figured for the first time. Galilia petricola Komai & Tsuchida, 2014, is recorded on the basis of a larger specimen, and distinguishing features with its only congener, G. narusei Ng & Richer de Forges, 2007, reappraised. Nursia rhomboidalis (Miers, 1879), previously known only from Japan, Korea, and mainland China, is also recorded from Taiwan. Myra fugax (Fabricius, 1798) is now formally recorded from Taiwan, and female characters identified to help separate the three known Taiwanese species of Myra.
Rodrigues, Felipe Tavares; Klemig, Larissa Raquel; Cardozo, Marcos Roberto Pereira; Alves, Paulo Cesar; Aguiar, Valéria Magalhães; Lessa, Claudia Soares
ABSTRACT Most breast cancers originate in the ductal epithelium and are referred to as invasive ductal carcinoma. In this study we report on the clinical procedures adopted to diagnose myiasis in association with infiltrating metastatic breast carcinoma in a female patient. A 41 years old woman came to the Federal Hospital of Andaraí complaining of intense itching, warmth, redness and hardening of the breast, which had acquired the aspect of an orange peel. A lesion in the left breast was cavitated, dimpled, had fetid odor, and had fibrotic and infected air nodules filled with exudate and Dipteran larvae. The tissue was cleaned and 33 larvae were extracted. The patient was hospitalized and received Ivermectin. Eighteen of the larvae extracted from the patient were placed in 70% alcohol, and twelve were placed in a container with sterile wood shavings under controlled conditions until they metamorphosed into adults. The taxonomic identification of the flies revealed that the culprit was Cochliomyia hominivorax. A histopathological exam conducted three months earlier had revealed infiltrating ductal carcinoma. Two months after the myiasis treatment, the breast tissue had healed. The patient had waited ten days from the onset of the myiasis to seek treatment, and that delay interfered negatively in the prognosis of both the neoplasm and the myiasis. This study is relevant to public health in view of the strong social impact of myiasis. PMID:28591263
Touré, S M
A simplified list of the principal Diptera capable of causing myiasis is followed by a brief presentation of the biology, lesions inflicted, and methods of treatment and control of the myiases of economic importance. Cochliomyiasis caused by Cochliomyia hominivorax is of greatest interest, in view of the damage and losses caused by this disease. A brief account of the outbreak of infestation in Libya illustrates the danger of this parasite. Other important traumatic myiases are described: that due to Chrysomya bezziana, which causes an African myiasis similar to cochliomyiasis, and those due to Lucilia cuprina and related species. Hypodermyiasis (warble fly infestation) and oestrosis (nasal bot fly infestation in sheep) still cause major economic losses in domestic animals, justifying their inclusion in control campaigns. The same applies to stomach bot flies of the family Gasterophilidae. The account of each myiasis includes notes on parasiticides which have been found to be effective. Given the rapidity with which a parasite can now be transported from one continent to another, it is important for Veterinary Services to be well-informed and vigilant.
Reichard, R E
Two examples of area-wide programmes, employing the sterile insect technique (SIT), which have eradicated a parasite and a disease vector common to domestic and wild animals are described. New World screwworm (NWS), Cochliomyia hominivorax, caused significant morbidity and mortality of livestock and wild mammals in tropical and subtropical areas of America before eradication was achieved in North America using the SIT and other components of an integrated pest management (IPM) programme. Movement of wild as well as domestic animals from an area which is infested with screwworm to a free area requires prophylactic treatment. Tsetse fly-borne trypanosomosis has an immense influence on the distribution of people and livestock in Africa. The immunotolerance of wildlife to the parasites is an important factor in maintaining some areas livestock free as wildlife refuges. Slaughter has ceased of wild hoofstock species considered to be disease reservoirs for control purposes. The SIT, combined with other IPM measures, has resulted in the eradication of the tsetse fly and trypanosomosis from Zanzibar. Other programmes in Africa are underway. Microbial 'biopesticides' have also been employed successfully against plant insect pests and some vectors of human disease. It seems likely that for the immediate future, wildlife may benefit from area-wide biological control programmes, intended mainly to protect humans and/or domestic animals.
Chaudhury, M F; Zhu, J J; Skoda, S R
The sheep blowfly, Lucilia sericata Meigen (Diptera: Calliphoridae), causes sheep myiasis in various parts of the world. Female flies are attracted to sheep following various olfactory cues emanating from the sheep's body, and oviposit on suitable substrates on sheep ultimately causing myiasis. Earlier workers attempted to reduce fly population in the field, with some success, using traps baited with various attractants. This research was conducted to determine if L. sericata would respond to a recently developed synthetic attractant that has attracted gravid screwworms, Cochliomyia hominivorax Coquerel, and stimulated them to oviposit. Results of the laboratory bioassays demonstrated that gravid females L. sericata were attracted to substrates treated with the synthetic screwworm attractant composed of five compounds--dimethyl disulfide, dimethyl trisulfide, phenol, p-cresol, and indole. Tests with various combinations of these compounds suggest that the sulfur compounds and indole are the most important compounds to elicit attraction and stimulate oviposition, while phenol and p-cresol may have minor roles. Semiochemical baits based on these compounds may be useful in the field to trap gravid L. sericata. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.
Chaudhury, M F; Alvarez, L Alfredo; Velazquez, L Lopez
Absorbent recycled paper products were tested and compared with a sodium polyacrylate gelling agent, Aquatain, for solidifying larval diets of the screwworm, Cochliomyia hominivorax (Coquerel). A recycled newsprint product, Terra-Mulch, when mixed with water and dietary ingredients produced a diet medium of homogeneous texture that supported larval growth and development comparable to the standard diet prepared with the gelling agent. Biological yields as calculated from number of pupae per tray from both the diets were similar. Three other types of recycled paper products were tested. Although less homogeneous in texture, these diets were as good as the diet with Terra-Mulch. There were no significant differences in the mean larval and pupal weights, no. of pupae/tray, percent emergence, oviposition, percent hatch, and percent longevity. When tests were conducted through eight consecutive generations, no adverse effect was noticed in any of the life history parameters. Terra-Mulch is inexpensive and biodegradable compared with the gelling agent Aquatain that is expensive and not biodegradable. Replacing Aquatain with Terra-Mulch in the screwworm larval diet for mass rearing may result in an annual savings of more than half a million U.S. dollars in costs of material and labor.
Edman, R M; Linger, R J; Belikoff, E J; Li, F; Sze, S-H; Tarone, A M; Scott, M J
The New World screwworm fly, Cochliomyia hominivorax, and the Australian sheep blow fly, Lucilia cuprina, are major pests of livestock. The sterile insect technique was used to eradicate C. hominivorax from North and Central America. This involved area-wide releases of male and female flies that had been sterilized by radiation. Genetic systems have been developed for making 'male-only' strains that would improve the efficiency of genetic control of insect pests. One system involves induction of female lethality in embryos through activation of a pro-apoptotic gene by the tetracycline-dependent transactivator. Sex-specific expression is achieved using an intron from the transformer gene, which we previously isolated from several calliphorids. In the present study, we report the isolation of the promoters from the C. hominivorax slam and Lucilia sericata bnk cellularization genes and show that these promoters can drive expression of a GFP reporter gene in early embryos of transgenic L. cuprina. Additionally, we report the isolation of the L. sericata pro-apoptotic hid and rpr genes, identify conserved motifs in the encoded proteins and determine the relative expression of these genes at different stages of development. We show that widespread expression of the L. sericata pro-apoptotic genes was lethal in Drosophila melanogaster. The isolated gene promoters and pro-apoptotic genes could potentially be used to build transgenic embryonic sexing strains of calliphorid livestock pests.
Keremidis, Haralampos; Appel, Bernd; Menrath, Andrea; Tomuzia, Katharina; Normark, Magnus; Roffey, Roger; Knutsson, Rickard
This article presents a historical perspective on agroterrorism cases from 1945 until 2012. The threat groups and perpetrators associated with bio- and agroterrorism are clustered into several groups: apocalyptic sects, lone wolves, political groups, and religious groups. We used open-source information, and 4 biological agroterrorism cases are described: (1) in 1952, Mau Mau poisoned cattle in Kenya by using a plant toxin from the African milk bush plant; (2) in 1985, the USDA claimed that Mexican contract workers were involved in deliberately spreading screwworm (Cochliomyia hominivorax) among livestock; (3) in 2000, Palestinian media reported that Israeli settlers released sewer water into Palestinian agricultural fields; and (4) in 2011, a person was sentenced to prison after threatening US and UK livestock with the deliberate spread of foot-and-mouth disease virus. All 4 cases can be assigned to political groups. These cases have not attracted much attention in literature nor in the public media, and the credibility of the sources of information varies. We concluded that agroterrorism has not been a problem during the period studied. Lessons learned from the few cases have generated awareness about the fact that nontypical biological weapons and non-high-risk agents, such as African milk bush, screwworm, and sewer water, have been used by attackers to influence local decision makers. This review will be useful in improving future preparedness planning and developing countermeasures.
Chaudhury, M F; Skoda, S R
A highly absorbent cellulose fiber from recycled paper was tested and compared with a polyacrylate gelling agent, Aquatain, normally used for bulking and solidifying larval rearing medium of screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). The absorbent fiber, when mixed with water and dietary ingredients, produced a diet medium of homogeneous texture that supported larval growth and development comparable with the standard gelled diet. Larval and pupal weights from two concentrations of cellulose fiber-based diet were significantly higher than those obtained using gelled diet. The number of pupae per tray, percentage of adult emergence, oviposition, percentage of egg hatch, and adult longevity obtained from the insects reared in the cellulose fiber-based diet were comparable or slightly better than the biological parameters recorded from flies reared in the gelled diet. Moreover, results indicate that a lesser amount of the cellulose fiber-based diet than the normal amount of gelled diet per tray would support normal larval growth. Physical properties and texture of the new diet seem to allow the larvae to move and feed more freely than they do on the semisolid gelled diet, resulting in less wasted diet. The cellulose fiber is biodegradable and inexpensive, whereas the polyacrylate gel polymer is not biodegradable and is relatively expensive. Replacing gel with cellulose fiber in the screwworm larval diet for mass rearing should result in substantial cost savings in material and labor as well as eliminating concern of environmental pollution due to diet waste disposal.
Morgan, E R; Clare, E L; Jefferies, R; Stevens, J R
SUMMARY Molecular phylogeography has revolutionised our ability to infer past biogeographic events from cross-sectional data on current parasite populations. In ecological parasitology, this approach has been used to address fundamental questions concerning host-parasite co-evolution and geographic patterns of spread, and has raised many technical issues and problems of interpretation. For applied parasitologists, the added complexity inherent in adding population genetic structure to perceived parasite distributions can sometimes seem to cloud rather than clarify approaches to control. In this paper, we use case studies firstly to illustrate the potential extent of cryptic diversity in parasite and parasitoid populations, secondly to consider how anthropogenic influences including movement of domestic animals affect the geographic distribution and host associations of parasite genotypes, and thirdly to explore the applied relevance of these processes to parasites of socio-economic importance. The contribution of phylogeographic approaches to deeper understanding of parasite biology in these cases is assessed. Thus, molecular data on the emerging parasites Angiostrongylus vasorum in dogs and wild canids, and the myiasis-causing flies Lucilia spp. in sheep and Cochliomyia hominovorax in humans, lead to clear implications for control efforts to limit global spread. Broader applications of molecular phylogeography to understanding parasite distributions in an era of rapid global change are also discussed.
Fresia, Pablo; Azeredo-Espin, Ana Maria L; Lyra, Mariana L
Insect pest phylogeography might be shaped both by biogeographic events and by human influence. Here, we conducted an approximate Bayesian computation (ABC) analysis to investigate the phylogeography of the New World screwworm fly, Cochliomyia hominivorax, with the aim of understanding its population history and its order and time of divergence. Our ABC analysis supports that populations spread from North to South in the Americas, in at least two different moments. The first split occurred between the North/Central American and South American populations in the end of the Last Glacial Maximum (15,300-19,000 YBP). The second split occurred between the North and South Amazonian populations in the transition between the Pleistocene and the Holocene eras (9,100-11,000 YBP). The species also experienced population expansion. Phylogenetic analysis likewise suggests this north to south colonization and Maxent models suggest an increase in the number of suitable areas in South America from the past to present. We found that the phylogeographic patterns observed in C. hominivorax cannot be explained only by climatic oscillations and can be connected to host population histories. Interestingly we found these patterns are very coincident with general patterns of ancient human movements in the Americas, suggesting that humans might have played a crucial role in shaping the distribution and population structure of this insect pest. This work presents the first hypothesis test regarding the processes that shaped the current phylogeographic structure of C. hominivorax and represents an alternate perspective on investigating the problem of insect pests.
Alford, John G.
The Sterile Insect Release Method (SIRM) is used as a biological control for invasive insect species. SIRM involves introducing large quantities of sterilized male insects into a wild population of invading insects. A fertile/sterile mating produces offspring that are not viable and the wild insect population will eventually be eradicated. A U.S. government program maintains a permanent sterile fly barrier zone in the Darien Gap between Panama and Columbia to control the screwworm fly (Cochliomyia Hominivorax), an insect that feeds off of living tissue in mammals and has devastating effects on livestock. This barrier zone is maintained by regular releases of massive quantities of sterilized male screwworm flies from aircraft. We analyze a reaction-diffusion model of the Darien Gap barrier zone. Simulations of the model equations yield two types of spatially inhomogeneous steady-state solutions representing a sterile fly barrier that does not prevent invasion and a barrier that does prevent invasion. We investigate steady-state solutions using both phase plane methods and monotone iteration methods and describe how barrier width and the sterile fly release rate affects steady-state behavior.
Chaudhury, M F; Chen, H; Sagel, A; Skoda, S R
Spray-dried whole bovine blood, dry poultry egg, and a dry milk substitute are the constituents of the artificial diet currently used for mass rearing screwworm larvae, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). Due to high cost and uncertainty of the commercial supply of spray-dried blood, research was conducted to identify alternative, locally available, inexpensive, dietary ingredients which could reduce cost of rearing and eliminate concerns of short supply. Experimental diets were prepared without blood component and with various ratios of bovine blood or blood cell product and defatted soy flour. Results indicate that spray-dried bovine blood can be replaced by a readily available and less expensive blood cell product. When the quantity of whole dried blood or blood cell component was reduced or removed completely from the diet, the larvae did not feed adequately, resulting in high mortality. Those larvae that survived produced pupae that were of unacceptable quality. When the milk product was replaced by soy flour, pupae were slightly smaller than those reared using the current diet; however, replacement of egg product with soy flour produced even smaller pupae. Longevity of adult flies that emerged from these small pupae was short and the females deposited few eggs. These results indicate that soy flour cannot replace the blood component from the diet, but can replace the milk product successfully. It is likely that some factor or a combination of factors in the blood act as feeding stimulants, without which larvae are unable to feed normally, resulting in high larval mortality.
Sagel, Agustin; Phillips, Pamela; Chaudhury, Muhammad; Skoda, Steven
Mass production, sterilization, and release of screwworms (Cochliomyia hominivorax (Coquerel)) that were competitive in the field significantly contributed to the successful application of the sterile insect technique for eradication of screwworms from continental North America. Metabolic byproducts resulting from protein-rich diets required for larval screwworms lead to ammonia liberation, sometimes at high levels, within the mass rearing facility. Until recently a sodium polyacrylate gel bulking agent was used for the larval media and adsorbed much of the ammonia. A need to replace the gel with an environmentally "friendly" bulking agent, while not increasing ammonia levels in the rearing facility, led to a series of experiments with the objective of developing procedures to reduce ammonia emissions from the larval media bulked with cellulose fiber. Additives of ammonia-converting bacteria, potassium permanganate, and Yucca schidigera Roezl ex Otrgies powder extract, previously reported to reduce ammonia levels in organic environments, were evaluated. Ammonia-converting bacteria did not have a positive effect. Addition of Y. schidigera powder extract (∼1% of total volume), potassium permanganate (∼250 ppm), and a combination of these two additives (at these same concentrations) kept ammonia at equivalent levels as when larval media was bulked with gel. Potassium permanganate also had sufficient antimicrobial properties that the use of formaldehyde in the diet was not necessary. Further testing is needed, at a mass rearing level, before full implementation into the screwworm eradication program.
Chaudhury, M F; Sagel, A; Skoda, S R
The waste artificial larval rearing media of New World screwworms, Cochliomyia hominivorax (Coquerel) were evaluated to determine their effectiveness as oviposition attractants. Various concentrations of waste larval media resulting from rearing screwworm larvae in gel and cellulose fiber-based artificial diets tested over a 4-wk period attracted varying number of gravid screwworm flies to oviposit. Three-day-old waste medium with concentrations of 10 and 25% were most attractive to gravid female flies for oviposition and resulted in the most oviposition. One and 7-d-old wastes at all concentrations were less attractive for oviposition than the 3d-old media. The fresh (0-d-old), 14-d- and 28-d-old waste media were the least attractive substrates for oviposition. The waste from the cellulose fiber-based diet resulted in significantly more oviposition compared with waste from the gel-based diet. Microorganisms growing in the waste media probably produce metabolites that attract gravid screwworm flies to oviposit. Use of the waste products of appropriate age and dilution as oviposition substrates would enhance oviposition in mass production colony cages.
Dawadi, B R; Sherpa, M T; Shrestha, R
Myiasis occurs in humans and vertebrate animals mostly due to dipterous flies of the genera Chrysomyia and Cochliomyia. There have been reported cases of myiasis of skin, breast, intestine, eyes and ear but very few cases of vulvar myiasis have been reported in the medical literature. We report a case of a 20 years old female with vulvar myiasis. A 20-year old unmarried girl presented to our hospital with complains of vaginal discharge and vulvar irritation for 3 days with evidence of some worms around her vulvar region. Her menstrual history revealed the use of folded cloths instead of sanitary pads during her menstruation. Moreover, she washed those clothes with water and hanged them in the clothesline outside to let them dry before reuse. Examination of the external genitalia multiple ulcers measuring 1X1 cm on the inner surface of the labia minora, heavily infested with large number of maggots. The patient was admitted for 4 days during which she was given antibiotics, analgesics and anti-inflammatory medication. Daily local asepsis of the wound with antiseptic solution, betadine was done. We removed more than 30 larvae by the fourth day after which no more larvae were seen and she gradually recovered in the following days. It is our opinion that the cause of vulvar myiasis in our patient was her poor sanitary hygiene. Simple measures such as washing clothes thoroughly, drying and ironing of clothes are also necessary to reduce the risk of this human myiasis.
Azeredo-Espin, Ana Maria L.
Insect pest phylogeography might be shaped both by biogeographic events and by human influence. Here, we conducted an approximate Bayesian computation (ABC) analysis to investigate the phylogeography of the New World screwworm fly, Cochliomyia hominivorax, with the aim of understanding its population history and its order and time of divergence. Our ABC analysis supports that populations spread from North to South in the Americas, in at least two different moments. The first split occurred between the North/Central American and South American populations in the end of the Last Glacial Maximum (15,300-19,000 YBP). The second split occurred between the North and South Amazonian populations in the transition between the Pleistocene and the Holocene eras (9,100-11,000 YBP). The species also experienced population expansion. Phylogenetic analysis likewise suggests this north to south colonization and Maxent models suggest an increase in the number of suitable areas in South America from the past to present. We found that the phylogeographic patterns observed in C. hominivorax cannot be explained only by climatic oscillations and can be connected to host population histories. Interestingly we found these patterns are very coincident with general patterns of ancient human movements in the Americas, suggesting that humans might have played a crucial role in shaping the distribution and population structure of this insect pest. This work presents the first hypothesis test regarding the processes that shaped the current phylogeographic structure of C. hominivorax and represents an alternate perspective on investigating the problem of insect pests. PMID:24098436
de Azeredo-Espin, Ana Maria Lima; Lessinger, Ana Cláudia
"Myiasis-causing flies" is a generic term that includes species from numerous dipteran families, mainly Calliphoridae and Oestridae, of which blowflies, screwworm flies and botflies are among the most important. This group of flies is characterized by the ability of their larvae to develop in animal flesh. When the host is a live vertebrate, such parasitism by dipterous larvae is known as primary myiasis. Myiasis-causing flies can be classified as saprophagous (free-living species), facultative or obligate parasites. Many of these flies are of great medical and veterinary importance in Brazil because of their role as key livestock insect-pests and vectors of pathogens, in addition to being considered important legal evidence in forensic entomology. The characterization of myiasis-causing flies using molecular markers to study mtDNA (by RFLP) and nuclear DNA (by RAPD and microsatellite) has been used to identify the evolutionary mechanisms responsible for specific patterns of genetic variability. These approaches have been successfully used to analyze the population structures of the New World screwworm fly Cochliomyia hominivorax and the botfly Dermatobia hominis. In this review, various aspects of the organization, evolution and potential applications of the mitochondrial genome of myiasis-causing flies in Brazil, and the analysis of nuclear markers in genetic studies of populations, are discussed.
Canty, Roy; Ruzzier, Enrico
Abstract Background Chrysomelid beetles associated with willow (Salix spp.) were surveyed at 41 sites across Europe, from Greece (lat. 38.8 °N) to arctic Norway (lat. 69.7 °N). New information In all, 34 willow-associated chrysomelid species were encountered, of which eight were very abundant. The abundant species were: Crepidodera aurata Marsham, 1802 at 27 sites, Phratora vitellinae (Linnaeus, 1758) at 21 sites, Galerucella lineola (Fabricius, 1781) at 19 sites, Crepidodera fulvicornis (Fabricius, 1792) at 19 sites, Plagiodera versicolora (Laicharting, 1781) at 11 sites, Crepidodera plutus (Latreille, 1804) at nine sites, Chrysomela vigintipunctata Scopoli, 1763 at nine sites and Gonioctena pallida (Linnaeus, 1758) at eight sites. The mean number of willow associated chrysomelid morphospecies at each site was 4.2. Around 20% of the total variance in chrysomelid distribution could be accounted for by latitude, but this is mainly due to distinctive occurrence patterns at the northern and southern parts of the transect. There was a paucity of chrysomelids at Greek sites and a distinctively northern faunal composition at sites north of Poland. Considerable site-to-site variation in colour was noted, except in G. lineola, which was chromatically invariant. PMID:27956853
Qubaiová, Jarin; Růžička, Jan; Šípková, Hana
Abstract The genus Ablattaria Reitter, 1884 (Coleoptera: Silphidae: Silphinae) is revised. Four taxa are recognized as valid species: Ablattaria arenaria (Kraatz, 1876), Ablattaria cribrata (Ménétries, 1832), Ablattaria laevigata (Fabricius, 1775) and Ablattaria subtriangula Reitter, 1905. Ablattaria laevigata var. meridionalis Ganglbauer, 1899 is newly treated as a junior subjective synonym of Ablattaria laevigata. Lectotypes are designated for Phosphuga arenaria Kraatz, 1876, Ablattaria arenaria var. punctigera Reitter, 1884, Ablattaria arenaria var. alleoni Portevin, 1926, Silpha cribrata Ménétries, 1832, Silpha laevigata Fabricius, 1775, Silpha gibba Brullé, 1832, Ablattaria gibba var. costulata Portevin, 1926, Ablattaria gibba var. distinguenda Portevin, 1926, Ablattaria gibba var. punctata Portevin, 1926 and Ablattaria subtriangula Reitter, 1905. The distribution of all taxa is mapped, based on material examined. Geometric morphometric methods were used to evaluate shape variability in Ablattaria. Results indicated sexual dimorphism in all species. Shape inconsistency was found between the sexes of all taxa when tested independently. The first two relative warp axes indicated 65.17% shape variation in males and 65.72% in females. Canonical variate analysis separated the taxa studied. There was minimal overlap between some groups in both sexes. Differences in body shape between populations of Ablattaria laevigata from Central Europe, Italy and Greece + Turkey were also examined. Relative warps implied 58.01% shape variability on both axes in males and 64.78% in females. CVA revealed noticeable overlaps between the groups, although the Italian population demonstrated a higher separation in both sexes. PMID:25685005
Dolfi, A; Bianchi, F; Lupetti, M; Michelucci, S
The aim of the present study was to evaluate the influence of intestinal material on the maturing of B lymphocytes in the bursa of Fabricius and on the antibody response to SRBC. Experiments were carried out on chicks whose burso-cloacal stalk had been ligated at hatching, in order to avoid any contact between the bursal tissues and intestinal material. Chicks subjected to a sham operation, or not operated at all, were used as controls. Results obtained by immunoperoxidase test, using an anti-chicken Ig antiserum, indicate that burso-cloacal stalk ligature leads to an increase in B cells in the bursa of Fabricius 20 days after the operation and a slight decrease after 60 days. The response to SRBC reveals an increase after 20 days, though no variations are found with respect to controls after 60 days. Thus, B lymphocytes mature independently of intestinal stimuli. Furthermore, observations carried out at the age of 20 days indicate that B lymphocytes disappear almost completely from the area of cloacal lymphoid infiltration in chicks with a ligated bursa. It is hypothesised that together with a systemic peripheralisation of B lymphocytes in secondary lymphoid organs and in blood, there exists an intramural local peripheralisation, which is interrupted by ligature of the burso-cloacal stalk. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 PMID:2621141
Broad dissemination of Histomonas meleagridis determined by the detection of nucleic acid in different organs after experimental infection of turkeys and specified pathogen-free chickens using a mono-eukaryotic culture of the parasite.
Grabensteiner, E; Liebhart, D; Weissenböck, H; Hess, M
Histomonas meleagridis, a flagellated protozoan parasite, is the causative agent of histomonosis (syn. histomoniasis, blackhead) in turkeys and chickens. The organs primarily affected by the parasite are the caeca and the liver. Until now, only few reports exist in which the parasite has been diagnosed in tissues other than those mentioned above. Hence, the aim of this study was to perform a systematic investigation of various organs of turkeys and specified pathogen-free chickens following an experimental infection with a mono-eukaryotic culture of Histomonas meleagridis in order to determine the dissemination of the flagellate in infected birds. Molecular methods like PCR and in situ hybridization were used for this purpose. For the first time, the DNA of the parasite could be detected in 13 different organs of infected turkeys by PCR including the proventriculus, duodenum, jejunum, caeca, pancreas, bursa of Fabricius, liver, kidney, spleen, heart, lung, thymus and the brain. Most of these findings were further confirmed by in situ hybridization. In contrast to the turkeys that all died shortly after the infection, all of the chickens survived without displaying any clinical symptoms. Even at necropsy, only mild pathological changes were observed in the caeca. Nevertheless, the parasite could also be detected in various organs of these birds, namely the caeca, bursa of Fabricius, kidney, heart and the brain.
Wysocki, Michał; Saganiak, Karolina; Zwinczewska, Helena; Roy, Joyeeta; Tomaszewski, Krzysztof A; Walocha, Jerzy A
The demand for anatomical illustrations in the early modern period coincided with a scientific revolution. Starting out as a servant, Iulius Casserius became a great anatomist, who challenged the Galenic doctrine. The aim of this paper is to honor his memory and recreate the stylism of his anatomical illustrations. Online databases were searched for articles and original works. A medical graphic designer then recreated the figures presented in the article. Casserius was born around 1552. After moving to Padua, he served Fabricius in performing dissections. Obtaining his medical degree, he began working as an anatomical dissector and surgeon, later giving private anatomy lectures to students. He published De Vocis Auditusque and Pentaestheseion, and then became the lecturer of Surgery. In 1616, Casserius started his first Anatomy course and then died suddenly, at the height of his career. From the sixteenth century, illustrative techniques began focusing less upon artistry in favor of precise depictions of anatomical structures. Fabricius is considered to have used a strict scientific approach to illustrations for the first time. Anatomists of subsequent generations would still frequently use artistry in illustrations. Despite Casserius' mixed accuracy and artistry, his plates mark a new epoch in anatomic representation. Casserius left numerous eponyms and depicted, for the first time, many anatomical structures. Reprints in textbooks in the centuries following show convincing evidence of his success. Casserius contributed to medical education by taking the theatricality out of anatomy. Our article is a tribute to Casserius's achievements and depicts the revolution brought forth by a pioneer of his times.
de Souza, Paulo Ricardo Barbosa; Guillermo-Ferreira, Rhainer
Abstract Butterflies and moths are found in all terrestrial environments and require efforts for a better understanding of its mega-diversity. These taxa have been the subject of several studies involving phylogeny, ecology and environmental impacts. Nevertheless, several areas in the tropics remain unexplored, resulting in gaps in the taxonomic composition and distribution of butterflies in endemic environments. Therefore, a survey of the butterfly fauna of the Bodoquena Plateau in Brazil was conducted. This area consists of tropical Atlantic Forests, with marginal influences of Savannah, Chaco and Pantanal. Sampling was carried out in 20 locations using Van Someren Rydon traps and insect nets between November 2009 and April 2015. Active collection of individuals was conducted from 9:00 to 17:00h, totaling 240 hours of sampling effort. In total, we registered 768 individuals belonging to 146 species of 98 genera, six families and 18 subfamilies. Nymphalidae was the richest family (84 species), followed by Hesperiidae (22 species), Riodinidae (14 species), Pieridae (12) Papilionidae (11 species) and Lycaenidae (five species). We sampled 239 nymphalids in traps, with 48 species, 30 genera, 15 tribes and five subfamilies. The most common species were Eunica macris (Godart, 1824), Dynamine artemisia (Fabricius, 1793) and Memphis moruus (Fabricius, 1775). Therefore, this study contributes to the knowledge of the Neotropical butterfly diversity and distribution, providing 37 new records and supporting the use of wildlife inventories as important tools for the knowledge of tropical forests biodiversity and conservation. PMID:26798308
Zhao, Chengquan; Nguyen, Tung; Liu, Lide; Sacco, Randy E.; Brogden, Kim A.; Lehrer, Robert I.
Gallinacin-3 and gallopavin-1 (GPV-1) are newly characterized, epithelial β-defensins of the chicken (Gallus gallus) and turkey (Meleagris gallopavo), respectively. In normal chickens, the expression of gallinacin-3 was especially prominent in the tongue, bursa of Fabricius, and trachea. It also occurred in other organs, including the skin, esophagus, air sacs, large intestine, and kidney. Tracheal expression of gallinacin-3 increased significantly after experimental infection of chickens with Haemophilus paragallinarum, whereas its expression in the tongue, esophagus, and bursa of Fabricius was unaffected. The precursor of gallinacin-3 contained a long C-terminal extension not present in the prepropeptide. By comparing the cDNA sequences of gallinacin-3 and GPV-1, we concluded that a 2-nucleotide insertion into the gallinacin-3 gene had induced a frameshift that read through the original stop codon and allowed the chicken propeptide to lengthen. The striking structural resemblance of the precursors of β-defensins to those of crotamines (highly toxic peptides found in rattlesnake venom) supports their homology, even though defensins are specialized to kill microorganisms and crotamines are specialized to kill much larger prey. PMID:11254635
Komatsu, Noriyuki; Kishimoto, Toshio; Uchida, Akihiko; Ooi, Hong-Kean
A survey of cockroach fauna was carried out on the 3 inhabited islands of the Ogasawara chain island of Japan, namely, Chichijima island, Hahajima island and Iwo island. Seven species, namely, Periplaneta americana (Linnaeus, 1758), Periplaneta australasiae (Fabricius, 1775), Blattella lituricollis (Walker, 1868), Onychostylus vilis (Brunner von Wattenwyl, 1865), Supella longipalpa (Fabricius, 1798), Pycnoscelus surinamensis (Linnaeus, 1758) and Opisthoplatia orientalis (Burmeister, 1838), were collected on Chichijima island. Four species, namely, P. americana, P. australasiae, O. vilis and P. surinamensis were collected on Hahajima island and 6 species, namely, P. americana, P. australasiae, B. lituricollis, O. vilis, P. surinamensis and Neostylopyga rhombifolia were collected on Iwo island. This is the first record of N. rhombifolia and Onychostylus orientalis on the Ogasawara chain islands. Our study increases the recorded taxon of cockroaches on the Ogasawara from 3 families, 5 genera 10 species to 4 families, 7 genera, 12 species. A list of the cockroach species on Ogasawara islands reported to date as well as a key for their identification is also presented. Periplaneta americana and P. australasiae, being the dominant species, together with S. longipalpa, were collected mostly in the indoor environment, indicating their preference for this habitat. Pycnoscelus surinamensis, which is considered as an outdoor insect has been found in semi-household environments such as greenhouse and shed, indicating their new adaptation to the changing environment.
Kavitha, Rajagopal; Nazni, Wasi Ahmad; Tan, Tian Chye; Lee, Han Lim; Azirun, Mohd Sofian
Forensic entomological specimens collected from human decedents during crime scene investigations in Malaysia in the past 6 years (2005-2010) are reviewed. A total of 80 cases were recorded and 93 specimens were collected. From these specimens, 10 species of cyclorrphagic flies were identified, consisting of Chrysomya rufifacies (Macquart) -38 specimens (40.86%), Chrysomya megacephala (Fabricius) -36 specimens (38.70%), Chrysomya villeneuvi (Patton) -2 specimens (2.15%), Chrysomya nigripes (Aubertin) -2 specimens (2.15%), Chrysomya pinguis (Walker) -1 specimen (1.08%), Hermetia illucens (Linnaeus) -1 specimen (1.08%), Hemipyrellia liguriens (Wiedemann) -5 specimens (5.37%), Synthesiomyia nudiseta (Wulp) -1 specimen (1.08%), Megaselia scalaris (Loew)-1 specimen (1.08%) and Sarcophaga ruficornis (Fabricius) -4 specimens (4.30%). In two specimens (2.15%), the maggots were not identifiable. Ch. megacephala and Ch. rufifacies were the commonest species found in human decedents from three different ecological habitats. S. nudiseta is an uncommon species found only on human cadavers from indoors. A total of 75 cases (93.75%) had a single fly infestation and 5 cases (6.25%) had double fly infestation. In conclusion, although large numbers of fly species were found on human decedents, the predominant species are still those of Chrysomya. Copyright © 2013 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.
Alipour, Fahimeh; Hassanabadi, Ahmad; Golian, Abolghasem; Nassiri-Moghaddam, Hassan
The effect of dietary thyme-oil extract (TOE) supplementation on immune functions of broilers were assessed by feeding graded levels (50, 100, 200, or 400 ppm) of TOE to male broiler chicks during a 42-d feeding trial compared with negative- or positive-control diets. Dietary control treatments included a negative-control diet with no feed-additive supplementation and 2 positive-control groups supplemented with either virginiamycin or zinc bacitracin. In total, 300 1-day-old Ross × Ross male broilers were randomly assigned to 6 dietary treatments that consisted of 5 replicates of 10 birds each. On d 21 and 42, 2 birds from each replicate were killed by cervical cutting to measure the relative weights of spleen and bursa of Fabricius. At 25 d of age, chicks were injected with 0.5 mL of 10% SRBC suspension. Broilers fed with 200 ppm of TOE had heavier weights of bursa of Fabricius than those fed other dietary treatments at d 42 of age. Furthermore, dietary inclusion of 100 ppm of TOE resulted in higher (P < 0.05) total immunoglobulin response in primary antibody titer against sheep erythrocytes compared with other dietary treatments. On the other hand, diet modifications had no significant effect on blood leukocyte subpopulations and heterophil-to-lymphocyte ratio. These results suggest that dietary supplementation with TOE, especially at the level of 100 ppm, can improve immunological responses of broiler chicks.
Echeverry, H; Yitbarek, A; Munyaka, P; Alizadeh, M; Cleaver, A; Camelo-Jaimes, G; Wang, P; O, K; Rodriguez-Lecompte, J C
The effect of organic trace mineral supplementation on performance, intestinal morphology, immune organ weights (bursa of Fabricius and spleen), expression of innate immune response related genes, blood heterophils/lymphocytes ratio, chemical metabolic panel, natural antibodies (IgG), and oxidative stress of broiler chickens was studied. A total of 1,080 day-old male broilers were assigned to 1 of 3 dietary treatments, which included basal diet with Monensin (control), control diet supplemented with bacitracin methylene disalicylate (BMD), and BMD diet supplemented with organic trace minerals (OTM). No difference in feed conversion ratio was observed among treatments; ileum histomorphological analysis showed a lower crypt depth, higher villi height/crypt depth ratio, and lower villi width in the OTM treatment compared to control. Furthermore, OTM treatment resulted in higher uric acid and lower plasma malondehaldehyde (MDA), indicating lower oxidative stress. Gene expression analysis showed that OTM treatment resulted in up-regulations of TLR2 bin the ileum, and TLR2b, TLR4, and IL-12p35 in the bursa of Fabricius, and down-regulation of TLR2b and TLR4 in the cecal tonsils. In the spleen, OTM treatment resulted in up-regulation of IL-10. In conclusion, OTM supplementation to broiler diets may have beneficial effects on intestinal development, immune system status, and survival by improving ileum histomorphological parameters, modulation of Toll-like receptors and anti-inflammatory cytokines, and decreasing level of MDA, which in conjunction could enhance health status.
Fu, Qiuling; Huang, Yu; Wan, Chunhe; Fu, Guanghua; Qi, Baomin; Cheng, Longfei; Shi, Shaohua; Chen, Hongmei; Liu, Rongchang; Chen, Zhenhai
In 2008, clinical cases of short beak and dwarfism syndrome (SBDS) caused by Muscovy duck parvovirus (MDPV) infection were found in mule duck and Taiwan white duck farms in Fujian, China. A MDPV LH strain causing duck SBDS without tongue protrusion was isolated in this study. Phylogenetic analysis show that the MDPV LH strain was clustered together with other MDPV strains, but divergent from GPV isolates. Two major fragment deletions were found in the inverted terminal repeats (ITR) of MDPV LH similar to the ones in the ITR of MDPV GX5, YY and SAAS-SHNH strains. To investigate the pathogenicity of the MDPV LH strain, virus infection of young mule ducks was performed. The infected ducks showed SBDS symptoms including retard growth and shorten beaks without tongue protrusion. Atrophy of thymus, spleen and bursa of Fabricius was identified in the infected ducks. The results show that MDPV LH strain is moderately pathogenic to mule duck, leading to occurrence of SBDS. As far as we know, it is the first study showing that SBDS without tongue protrusion, and atrophy of thymus, spleen and bursa of Fabricius possibly associated with immunosuppression were found in the MDPV-infected ducks. The established duck-MDPV-SBDS system will help us to further work on the virus pathogenesis and develop efficacious vaccine against MDPV infection. Copyright © 2017. Published by Elsevier Ltd.
de Souza, Paulo Ricardo Barbosa; Guillermo-Ferreira, Rhainer
Butterflies and moths are found in all terrestrial environments and require efforts for a better understanding of its mega-diversity. These taxa have been the subject of several studies involving phylogeny, ecology and environmental impacts. Nevertheless, several areas in the tropics remain unexplored, resulting in gaps in the taxonomic composition and distribution of butterflies in endemic environments. Therefore, a survey of the butterfly fauna of the Bodoquena Plateau in Brazil was conducted. This area consists of tropical Atlantic Forests, with marginal influences of Savannah, Chaco and Pantanal. Sampling was carried out in 20 locations using Van Someren Rydon traps and insect nets between November 2009 and April 2015. Active collection of individuals was conducted from 9:00 to 17:00h, totaling 240 hours of sampling effort. In total, we registered 768 individuals belonging to 146 species of 98 genera, six families and 18 subfamilies. Nymphalidae was the richest family (84 species), followed by Hesperiidae (22 species), Riodinidae (14 species), Pieridae (12) Papilionidae (11 species) and Lycaenidae (five species). We sampled 239 nymphalids in traps, with 48 species, 30 genera, 15 tribes and five subfamilies. The most common species were Eunica macris (Godart, 1824), Dynamine artemisia (Fabricius, 1793) and Memphis moruus (Fabricius, 1775). Therefore, this study contributes to the knowledge of the Neotropical butterfly diversity and distribution, providing 37 new records and supporting the use of wildlife inventories as important tools for the knowledge of tropical forests biodiversity and conservation.
Cardoso, Tereza C; Rosa, Ana C G; Astolphi, Rafael D; Vincente, Rafael M; Novais, Juliana B; Hirata, Karina Y; Luvizotto, Maria Cecilia R
The presence of the very virulent (vv) Brazilian strain of infectious bursal disease virus (IBDV) was determined in the bursa of Fabricius, thymus and liver of 2-week-old broilers from a flock with a higher than expected mortality. For this purpose, a direct in situ reverse transcriptase (RT)-linked polymerase chain reaction (PCR) method was developed using specific primers for vvIBDV. Unlabelled forward and reverse biotinylated oligonucleotides were used for RT-PCR in a one-step method and the respective products were revealed by a direct enzymatic reaction. The results were compared with those obtained by standard RT-PCR using general primers for IBDV and virus isolation. The virus isolation, RT-PCR and in situ RT-PCR revealed positive results on the bursa of Fabricius in 86%, 80% and 100%, respectively. The in situ RT-PCR detected vvIBDV in all tested thymus and liver samples, whereas the standard RT-PCR detected virus in 80% and 90% of the samples, respectively. After three consecutive passages on chicken embryonated eggs, IBDV was isolated from 64% of the thymus samples and 30% of the liver samples. In the present study, no classical or antigenic variants of IBDV were detected. The developed in situ RT-PCR assay was able to detect the very virulent strain of IBDV with a higher sensitivity than the conventional RT-PCR and virus isolation.
Feeding a diet contaminated with ochratoxin A for broiler chickens at the maximum level recommended by the EU for poultry feeds (0.1 mg/kg). 2. Effects on meat quality, oxidative stress, residues and histological traits.
Pozzo, L; Cavallarin, L; Antoniazzi, S; Guerre, P; Biasibetti, E; Capucchio, M T; Schiavone, A
The European Commission Recommendation 2006/576/EC indicates that the maximum tolerable level of ochratoxin A (OTA) in poultry feeds is 0.1 mg OTA/kg. Thirty-six 1-day-old male broiler chicks were divided into two groups, a control (basal diet) and an OTA (basal diet + 0.1 mg OTA/kg) group. The OTA concentration was quantified in serum, liver, kidney, breast and thigh samples. The thiobarbituric acid reactive substances (TBARS) content were evaluated in the liver, kidney, breast and thigh samples. The glutathione (GSH) content, and catalase (CAT) and superoxide dismutase (SOD) activity were measured in the liver and kidney samples. Histopathological traits were evaluated for the spleen, bursa of Fabricius and liver samples. Moreover, the chemical composition of the meat was analysed in breast and thigh samples. In the OTA diet-fed animals, a serum OTA concentration of 1.15 ± 0.35 ng/ml was found, and OTA was also detected in kidney and liver at 3.58 ± 0.85 ng OTA/g f.w. and 1.92 ± 0.21 ng OTA/g f.w., respectively. The TBARS content was higher in the kidney of the ochratoxin A group (1.53 ± 0.18 nmol/mg protein vs. 0.91 ± 0.25 nmol/mg protein). Feeding OTA at 0.1 mg OTA/kg also resulted in degenerative lesions in the spleen, bursa of Fabricius and liver. The maximum tolerable level of 0.1 mg OTA/kg, established for poultry feeds by the EU, represents a safe limit for the final consumer, because no OTA residues were found in breast and thigh meat. Even though no clinical signs were noticed in the birds fed the OTA-contaminated diet, moderate histological lesions were observed in the liver, spleen and bursa of Fabricius. Journal of Animal Physiology and Animal Nutrition © 2013 Blackwell Verlag GmbH.
A new Hermeuptychia (Lepidoptera, Nymphalidae, Satyrinae) is sympatric and synchronic with H. sosybius in southeast US coastal plains, while another new Hermeuptychia species - not hermes - inhabits south Texas and northeast Mexico.
Cong, Qian; Grishin, Nick V
Hermeuptychia intricata Grishin, sp. n. is described from the Brazos Bend State Park in Texas, United States, where it flies synchronously with Hermeuptychia sosybius (Fabricius, 1793). The two species differ strongly in both male and female genitalia and exhibit 3.5% difference in the COI barcode sequence of mitochondrial DNA. Setting such significant genitalic and genotypic differences aside, we were not able to find reliable wing pattern characters to tell a difference between the two species. This superficial similarity may explain why H. intricata, only distantly related to H. sosybius, has remained unnoticed until now, despite being widely distributed in the coastal plains from South Carolina to Texas, USA (and possibly to Costa Rica). Obscuring the presence of a cryptic species even further, wing patterns are variable in both butterflies and ventral eyespots vary from large to almost absent. To avoid confusion with the new species, neotype for Papilio sosybius Fabricius, 1793, a common butterfly that occurs across northeast US, is designated from Savannah, Georgia, USA. It secures the universally accepted traditional usage of this name. Furthermore, we find that DNA barcodes of Hermeuptychia specimens from the US, even those from extreme south Texas, are at least 4% different from those of H. hermes (Fabricius, 1775)-type locality Brazil: Rio de Janeiro-and suggest that the name H. hermes should not be used for USA populations, but rather reserved for the South American species. This conclusion is further supported by comparison of male genitalia. However, facies, genitalia and 2.1% different DNA barcodes set Hermeuptychia populations in the lower Rio Grande Valley of Texas apart from H. sosybius. These southern populations, also found in northeastern Mexico, are described here as Hermeuptychia hermybius Grishin, sp. n. (type locality Texas: Cameron County). While being phylogenetically closer to H. sosybius than to any other Hermeuptychia species, H
Ul-Hassan, Zahoor; Khan, Muhammad Zargham; Khan, Ahrar; Javed, Ijaz
This study aimed to evaluate the immunological status of progeny of hens kept on ochratoxin A (OTA)- and aflatoxin B(1) (AFB(1))-contaminated feed. For this purpose, White Leghorn (WL) layer breeder hens (40-weeks-of-age) were divided into six groups (A-F). Hens in Group A were fed a commercial layer ration while those in Groups B and C were kept on a diet amended with 3 and 5 mg OTA/Kg, respectively. Group D was fed a ration containing 5 mg AFB(1)/Kg, while hens in Groups E and F were kept on feed amended with OTA and AFB(1) each. All feedings were for 1, 2, or 3 weeks. Fertile eggs were set for hatching on a weekly basis to obtain progeny of each week separately. At 14 days-of-age, subsets of progeny were euthanized and the frequency of immunoglobulin(s)-bearing cells in their spleen and bursa of Fabricius assessed; at 16 days-of-age, other chicks in each set were utilized to determine their lymphoblastogenic responses against phytohemagglutinin (PHA-P). At 30 days-of-age, the final sub-set of chicks/group was euthanized and their peritoneal macrophages harvested for measurements of phagocytic potential and nitrite production. Relative weights of the bursa of Fabricius and of the spleen were significantly lower in the progeny of hens fed mycotoxin-contaminated diets for 14 and 21 days. The frequencies of IgA-, IgG-, and IgM-bearing cells were also significantly lower in the bursa of Fabricius and spleen of progeny chicks obtained from hens fed the OTA + AFB(1) mixed diet. Feeding contaminated diets to breeder hens also resulted in significantly lower responses to PHA-P. In addition, the percentages of peritoneal macrophages displaying phagocytosis of sheep red blood cells (SRBC), the number of SRBC/macrophage, and nitrite production were each significantly lower in cells from progeny chicks from OTA- and AFB(1)-fed hens. The findings of the present study indicated there were severe immunosuppressive effects in progeny chicks as a result of exposure of their
A new Hermeuptychia (Lepidoptera, Nymphalidae, Satyrinae) is sympatric and synchronic with H. sosybius in southeast US coastal plains, while another new Hermeuptychia species – not hermes – inhabits south Texas and northeast Mexico
Cong, Qian; Grishin, Nick V.
Abstract Hermeuptychia intricata Grishin, sp. n. is described from the Brazos Bend State Park in Texas, United States, where it flies synchronously with Hermeuptychia sosybius (Fabricius, 1793). The two species differ strongly in both male and female genitalia and exhibit 3.5% difference in the COI barcode sequence of mitochondrial DNA. Setting such significant genitalic and genotypic differences aside, we were not able to find reliable wing pattern characters to tell a difference between the two species. This superficial similarity may explain why H. intricata, only distantly related to H. sosybius, has remained unnoticed until now, despite being widely distributed in the coastal plains from South Carolina to Texas, USA (and possibly to Costa Rica). Obscuring the presence of a cryptic species even further, wing patterns are variable in both butterflies and ventral eyespots vary from large to almost absent. To avoid confusion with the new species, neotype for Papilio sosybius Fabricius, 1793, a common butterfly that occurs across northeast US, is designated from Savannah, Georgia, USA. It secures the universally accepted traditional usage of this name. Furthermore, we find that DNA barcodes of Hermeuptychia specimens from the US, even those from extreme south Texas, are at least 4% different from those of H. hermes (Fabricius, 1775)—type locality Brazil: Rio de Janeiro—and suggest that the name H. hermes should not be used for USA populations, but rather reserved for the South American species. This conclusion is further supported by comparison of male genitalia. However, facies, genitalia and 2.1% different DNA barcodes set Hermeuptychia populations in the lower Rio Grande Valley of Texas apart from H. sosybius. These southern populations, also found in northeastern Mexico, are described here as Hermeuptychia hermybius Grishin, sp. n. (type locality Texas: Cameron County). While being phylogenetically closer to H. sosybius than to any other Hermeuptychia
Chaudhury, M F; Skoda, S R; Sagel, A
Spray-dried whole bovine blood and a sodium polyacrylate polymer gel as a bulking and solidifying agent are among the constituents of the current larval diet for mass rearing screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). Locally available, inexpensive dietary materials could reduce rearing cost and address an uncertain commercial supply of spray-dried blood. We compared efficacy of diet prepared from fresh bovine blood after decoagulation with sodium citrate or ethylenediaminetetraacetic acid (EDTA) or after mechanical defibrination, with the diet containing spray-dried blood using either gel or cellulose fiber as the bulking and solidifying agent. Several life-history parameters were compared among insects reared on each of the blood and bulking agent diets combination. Diets containing citrated blood yielded the lightest larval and pupal weights and fewest pupae. EDTA-treated blood with the gel also caused reductions. EDTA-treated blood with fiber yielded screwworms that were heavier and more numerous than those from the diet with citrated blood but lighter than those from the control diet using spray-dried blood. A reduction in percentage of adults emerging from pupae occurred from diets with both bulking agents using citrated blood and the diet using EDTA mixed with the gel bulking agent. As a group, the cellulose-fiber diets performed better than the gel diets. Larval diet did not affect adult longevity, weight of the eggs deposited by the females that emerged or subsequent egg hatch. Parameter measurements of insects from both defibrinated blood diets were similar to those from the spray-dried blood diets, indicating that fresh, defibrinated bovine blood can successfully replace the dry blood in the screwworm rearing medium.
Ramakodi, Meganathan P; Singh, Baneshwar; Wells, Jeffrey D; Guerrero, Felix; Ray, David A
The availability of complete mitochondrial genome (mtgenome) data for Diptera, one of the largest metazoan orders, in public databases is limited. The advent of high throughput sequencing technology provides the potential to generate mtgenomes for many species affordably and quickly. However, these technologies need to be validated for dipterans as the members of this clade play important economic and research roles. Illumina and 454 sequencing platforms are widely used in genomic research involving non-model organisms. The Illumina platform has already been utilized for generating mitochondrial genomes without using conventional long range PCR for insects whereas the power of 454 sequencing for generating mitochondrial genome drafts without PCR has not yet been validated for insects. Thus, this study examines the utility of 454 sequencing approach for dipteran mtgenomic research. We generated complete or nearly complete mitochondrial genomes for Cochliomyia hominivorax, Haematobia irritans, Phormia regina and Sarcophaga crassipalpis using a 454 sequencing approach. Comparisons between newly obtained and existing assemblies for C. hominivorax and H. irritans revealed no major discrepancies and verified the utility of 454 sequencing for dipteran mitochondrial genomes. We also report the complete mitochondrial sequences for two forensically important flies, P. regina and S. crassipalpis, which could be used to provide useful information to legal personnel. Comparative analyses revealed that dipterans follow similar codon usage and nucleotide biases that could be due to mutational and selection pressures. This study illustrates the utility of 454 sequencing to obtain complete mitochondrial genomes for dipterans without the aid of conventional molecular techniques such as PCR and cloning and validates this method of mtgenome sequencing in arthropods.
Skoda, Steven R; Phillips, Pamela L; Sagel, Agustin; Chaudhury, Muhammad F
The sterile insect technique is used by the Comisión Panamá - Estados Unidos para la Erradicación y Prevención del Gusano Barrenador del Ganado (COPEG) to maintain a barrier at the border of Panama and Colombia to prevent screwworms, Cochliomyia hominivorax (Coquerel), from South America reinfesting North America. Before studying the distribution and persistence of sterilized, mass-produced screwworms released in the barrier zone, the utility of applying fluorescent dust (∼1.0 mg/fly) to pupae and to newly emerged adults was evaluated to determine the potential effect on fly survival. The flight ability of flies collected from two adult emergence/collection systems (enclosed towers and open chambers) and treated with low (∼0.20 mg/fly) or high (∼1.0 mg/fly) amounts of fluorescent powder was compared. The distribution and persistence of sterile screwworms marked with fluorescent powder (∼0.20 mg/fly), after collection from the same two adult emergence/collection systems, was compared after their release in the barrier zone. The results demonstrated that: 1) fluorescent dust did not negatively affect sterile screwworm longevity or flight ability; 2) no differences were detected between sterile flies collected from the two emergence systems; and 3) sterile screwworms distributed evenly in the barrier zone and persisted for > 6 d. This information was useful in implementing the use of a new sterile fly emergence/collection system and deploying a new strain by COPEG for the barrier zone maintenance program; it will be valuable for evaluating alternative release strategies of sterile screwworms by the eradication and barrier maintenance program. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.
Smith, J L; Wells, J D
Being able to efficiently differentiate between male and female individuals in the immature forms of insects allows for investigations into sexually dimorphic patterns of growth rates and gene expression. For species lacking sex-specific morphological characteristics during these periods, alternative methods must be devised. Commonly, isolation of sex determination genes reveals sex-specific band patterns and allows for markers that can be used in insect control. For blow flies, a family that includes flies of medical and forensic importance, sex has previously been identified in some members using the male-specific exon in the transformer gene. This gene is relatively conserved between members of the genera Cochliomyia and Lucilia (Diptera: Calliphoridae), and we isolated a portion of this gene in an additional forensically and medically important blow fly genus using the widespread Chrysomya megacephala (F.). We found a relatively high level of conservation between exons 1 and 2 of transformer and were able to amplify a region containing the male-specific exon in C. megacephala. A sex-specific molecular diagnostic test based on the presence of sexually dimorphic PCR product bands showed the expected genotype for adults and intrapuparial period specimens of known sex. The same result could be obtained from single third-instar larval specimens, opening up the possibility to not only determine if development rates are sex dependent, but also to investigate the development of sexually dimorphic traits of interest in C. megacephala. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: firstname.lastname@example.org.
Krafsur, E S; Lindquist, D A
The American screwworm, Cochliomyia hominivorax Coquerel, was detected in northwestern Libya in 1988. By August 1990, a screwworm epizootic extended over 26,000 km2 but collapsed in December and disappeared in April 1991. The relative contributions of winter weather and sterile insect releases on screwworm eradication in Libya were assessed by using temperature data and population demography. A screwworm overwintering threshold mean temperature of approximately 9.7 degrees C for 3 mo is supported strongly by published experimental and epizootiological data. In Libya, temperatures were above this overwintering threshold at Zawia, Sorman, and Tripoli weather stations. At Gharyan, in the interior highlands, mean winter temperatures from 1 December 1990 to 28 February 1991 were less than the putative overwintering threshold. No kill of screwworm pupae or adults was likely as a result of low temperatures near any weather station in the winter of 1990-1991. Evidence of screwworm overwintering in 1990-1991 was provided by the detection of a natural infestation in April and trap captures of numerous wild adult females in February, March, and April. Successful screwworm overwintering was highly probable in the coastal plain of Libya but unlikely in much of the interior highlands. The high rate of animal inspections by eradication personnel achieved as much as a 30% chance of detecting a screwworm case in domestic animals. Generation times and expectation of life among feral adult flies were estimated. Case reports for January to June 1991 were consistently less than estimates based on historical experience in Libya 1989-1990 and in Texas 1962-1983. Phenological simulations of sterile mating rates in feral screwworm flies supports the contention that sterile fly releases led to a greatly reduced case incidence from January to the time that eradication was declared in October 1991.
Phillips, Pamela L; Welch, John B; Kramer, Matthew
The distribution of screwworms, Cochliomyia hominivorax, (Coquerel) was studied in a seasonally moist lowland tropical forest in the Republic of Panama using a combination of field collections and satellite imagery. We found that different forest types could be distinguished and mapped using remotely sensed data. To determine the temporal and spatial distribution of flies, we collected flies coming to rotted liver at 82 sites in ten vegetation types (open areas, edge forest, dry scrub forest, forest successional stage 1, forest successional stage 2, forest successional stage 3, forest successional stage 4, forest successional stage 5, mature forests, palm swamp forest, and forest along streams) over three seasons (dry, transitional, wet). Nine of the vegetation types (excluding dry scrub forest) were identified and mapped using SPOT XS and Landsat 5 TM satellite data. Screwworm flies were most abundant during the transition from wet to dry season. Fly numbers were consistently higher in forest habitats, particularly those with trees 20-30 m in height and a fairly open canopy composed of many deciduous species that shed their leaves during the dry season. Screwworm numbers were also high in palm swamp forest, edge forest, and mature growth forest. Traps sampled in open areas had fewer flies and were unrelated to proximity to cattle. Females accounted for 88% of the total fly counts. This study further substantiates the importance of forests in the ecology and behavior of screwworm flies and demonstrates that remotely sensed data can be used to construct the spatial distribution of these flies in a tropical landscape. We discuss implications of this information to the screwworm eradication program.
Wetterer, James K.; Banko, Paul C.; Laniawe, Leona P.; Slotterback, John W.; Brenner, Gregory J.
Ant surveys were conducted at high elevations (1680-3140 m) on the western slope of Mauna Kea Volcano on the island of Hawai'i to detennine the extent of ant infestation in those highland communities and particularly to evaluate the potential threat of ants in the highlands to native Hawaiian species. Ants were surveyed at 10 long-tenn sampling sites. Ants were common on Mauna Kea up to 2000 m elevation, but densities quickly dropped off above that. Five species of ants were collected: Linepithema humile (Mayr), Cardiocondyla venustula Wheeler, Pheidole megacephala (Fabricius), Tetramorium bicarinatum (Nylander), and Monomorium pharaonis (Linnaeus). Other than L. humile, these collections on Mauna Kea are the highest recorded locales in the Hawaiian Islands.
Garris, G I; Scotland, K
Cattle, sheep, goats and horses were examined for ticks. Over 95% of Holstein cross-breeds, 28% of sheep (local mixed breeds) and 18% of goats (local mixed breeds) examined from 18 August to 4 September 1983 were infested with the southern cattle tick, Boophilus microplus Canestrini. About 90 and 17% of the horses examined were infested with the tropical horse tick, Anocentor nitens Neumann, and the tropical bont tick, Amblyomma variegatum Fabricius, respectively. The tropical bont tick was found infesting 10% of cattle in the Gros Islet area of St. Lucia. The tropical bont tick was also found associated with a severe skin disease, dermatophilosis, caused by the bacterium Dermatophilus congolensis, in 54% of the cattle infested by A. variegatum in the Gros Islet and Dauphin areas of St. Lucia.
Wu, Li; Liu, Jinxiong; Chen, Pucheng; Jiang, Yongping; Ding, Leilei; Lin, Yuan; Li, Qimeng; He, Xijun; Chen, Qiusheng; Chen, Hualan
In 2010, a novel Tembusu virus (TMUV) that caused a severe decrease in the egg production of ducks was isolated in southeast China. Given the novelty of this duck pathogen, little information is available regarding its pathogenesis. Here, we systematically investigated the replication kinetics of TMUV PTD2010 in adult male and female ducks. We found that PTD2010 was detectable in most of the parenchymatous organs as well as the oviduct and intestinal tract from days 1 to 7 after inoculation. Viral titers were maintained at high levels for at least 9 days in the spleen, kidney, bursa of Fabricius, brain, and ovary. No virus was detected in any of these organs or tissues at 18 days after inoculation. PTD2010, thus, causes systemic infections in male and female ducks; its replication kinetics show similar patterns in most organs, with the exception of the ovaries and testes. PMID:25215289
Duriš, Zdenek; Anker, Arthur
A new species of the alpheid shrimp genus Athanas Leach, 1814 is described based on a single specimen, an ovigerous female from Nha Trang Bay, Vietnam. Athanas manticolus sp. nov. differs from all other species of Athanas by the presence of a small post-rostral tubercle, combined with a minutely toothed rostrum, reduced extra-corneal teeth, and the absence of infra-corneal and supra-corneal teeth. In addition, the new species is characterised by its unique colour pattern, particularly by the red chromatophores disposed in randomly oriented, short, narrow streaks. As its name suggests, A. manticolus sp. nov. is commensally associated with the burrows of the nannosquillid mantis shrimp Bigelowina phalangium (Fabricius, 1798).
Koetz, Anna H.
Apis cerana Fabricius is endemic to most of Asia, where it has been used for honey production and pollination services for thousands of years. Since the 1980s, A. cerana has been introduced to areas outside its natural range (namely New Guinea, the Solomon Islands, and Australia), which sparked fears that it may become a pest species that could compete with, and negatively affect, native Australian fauna and flora, as well as commercially kept A. mellifera and commercial crops. This literature review is a response to these concerns and reviews what is known about the ecology and behaviour of A. cerana. Differences between temperate and tropical strains of A. cerana are reviewed, as are A. cerana pollination, competition between A. cerana and A. mellifera, and the impact and control strategies of introduced A. cerana, with a particular focus on gaps of current knowledge. PMID:26462524
Sánchez-Cordón, P J; Hervás, J; Chacón de Lara, F; Jahn, J; Salguero, F J; Gómez-Villamandos, J C
In this paper we report on an outbreak of reovirus, herpesvirus (Pacheco disease), and/or mycosis infection (Aspergillus spp. and Zygomyces spp.) affecting a batch of young African grey parrots (Psittacus erithacus), with 80% morbidity and 30% mortality. Study material was taken from five birds (four dead and one euthanatized) with a range of clinical symptoms (depression, diarrhea, respiratory symptoms). Diagnosis was confirmed by immunohistochemical detection of avian reovirus, electron microscopy, and virus isolation. Viral antigen of reovirus was detected mainly in large mononuclear cells in the bursa of Fabricius and the spleen, pancreas epithelial cells, and circulating cells; lymphoid organs displayed the largest number of immunopositive cells and severe lymphocyte depletion. Bacteriologic study was negative. Reovirus infection was common in all birds studied, whereas Pacheco disease and mycosis were found in only some, suggesting that reovirus could be the initial cause triggering the outbreak and facilitating infection by other agents and their swift spread through the batch.
Loktionov, Valery M; Lelej, Arkady S
Keys to 55 genera of spider wasps of Russia and neighbouring countries in females and males are given. Of them 34 genera are distributed in Russia. An annotated list of genera with type species and distribution data within Russia and biogeographical regions is given. The genus Xenaporus Ashmead, 1902 and X. eremocanus Wolf, 1990 are newly recorded from Russia. According to ICZN 1995 (Opinion 1820) new synonymy (valid name first) is proposed for the type species of genus Cryptocheilus Panzer, 1806: Sphex annulata Fabricius, 1798 (=Pompilus alternatus Lepeletier de Saint Fargeau, 1845, syn. nov.; =Pompilus comparatus Smith, 1855, syn. nov.; =Priocnemis culpabilis Costa, 1893, syn. nov.; Salius annulatilis Richards, 1935, syn. nov.).
Jood, S; Kapoor, A C; Singh, R
A significant decrease in essential amino acids of wheat, maize and sorghum was observed due to grain infestation caused by mixed populations of Trogoderma granarium Everts and Rhizopertha dominica Fabricius (50:50). Non-essential amino acids were also adversely affected. Among the essential amino acids, maximum reduction was found in methionine, isoleucine and lysine in infested wheat, maize and sorghum grains, respectively. Lysine, with lowest chemical score in uninfested and infested grains of three cereals, is the first limiting amino acid. Insect infestation caused significant (p < 0.05) reduction in the chemical score of all the essential amino acids, yet did not change the position of first and second limiting amino acids in wheat and sorghum. However, in case of maize, isoleucine became the second limiting amino acid. Infested grains also showed substantial reduction in essential amino acid index, calculated biological value and requirement index.
George, Kelly A; Archer, Melanie S; Toop, Tes
Worldwide research into nocturnal colonization by blowflies has produced many contradictory findings, prompting investigation specific to southeastern Australia. Initial experiments showed that blowfly colonization begins shortly after sunrise and continues until sunset; nocturnal colonization never occurred. Colonization peaks occurred at mid-morning, midday, and in the hours preceding sunset. In an additional experiment, wild blowflies were captured and placed in cages with colonization medium supplied nocturnally. Colonization occurred on four of five nights, and Calliphora augur (Fabricius) (Diptera: Calliphoridae) was the main species colonizing baits nocturnally. Results suggest that colonization is most likely to occur during warm weather and when flies are able to walk or crawl to bait. In particular, blowflies trapped within a confined space (such as a room or car) with warmer-than-ambient temperature may be stimulated to colonize nearby remains. Entomologists should consider these findings when estimating minimum postmortem interval under these environmental conditions.
Zhou, Guang Fang; Liu, Qing Tao; Zhou, Bin; Qiu, Ya Feng; Liu, Xiao Dong; Ma, Zhi Yong; Chen, Pu Yan
The bursa of Fabricius (BF) is the acknowledged central humoral immune organ in birds. Bursal septpeptide II (BSP-II) is an immunomodulatory bioactive peptide isolated from BF. To understand the effects of BSP-II on immune induction, gene expression profiles of hybridoma cells treated with BSP-II were evaluated. Pathway analysis showed that regulated genes were involved in cytokine-cytokine receptor interactions, T cell receptor signaling pathway, and pathway in cancer. It was observed that BSP-II reduced tumor cells proliferation and stimulated p53 expression. These results indicate potential mechanisms underlying the effects of the humoral immune system on immune induction, including antitumor activities. Our study has provided a novel insight into immunotherapeutic strategies for treating human tumors. PMID:25643804
Chazottes, Veronique; Hutchings, Pat; Osorno, Alicia
The components of bioerosion were investigated during ENCORE (The Effect of Nutrient Enrichment on Coral Reefs) over 2years of controlled additions of dissolved inorganic nitrogen and phosphorus. The study was carried out at One Tree Island, southern Great Barrier Reef, Australia. Rates of microbioerosion and external erosion by grazing were significantly higher at the enriched sites than at the control sites. Rates of accretion by coralline algae were also significantly higher at enriched sites. In contrast, rates of macroboring were not significantly different between enriched and control sites. This study highlights the importance of improving water quality on the reef to reduce rates of bioerosion given that quantities of dead coral substrates have recently substantially increased as a result of coral bleaching (Hughes et al., 2015) and several Crown of Thorns plagues (Fabricius et al., 2010; De'ath et al., 2012), on the Great Barrier Reef.
Webster, Reginald P.; Sweeney, Jon D.; DeMerchant, Ian; Bouchard, Patrice; Bousquet, Yves
Abstract Thirteen species of Tenebrionidae are newly reported for New Brunswick, Canada. Paratenetus punctatus Spinola, Pseudocistela brevis (Say), Mycetochara foveata (LeConte), and Xylopinus aenescens LeConte are recorded for the first time from the Maritime provinces. Platydema excavatum (Say) is removed from the faunal list of New Brunswick, and the presence of Platydema americanum Laporte and Brullé for the province is confirmed. This brings the total number of species of Tenebrionidae known from New Brunswick to 42. Two species of Zopheridae, Bitoma crenata Fabricius and Synchita fuliginosa Melsheimer, are newly recorded for New Brunswick, bringing the number of species known from the province to four. Bitoma crenata is new to the Maritime provinces. Collection and habitat data are presented for these species. PMID:22539897
Srinivasa Rao, M.; Venkateswarlu, B.
Intercropping is one of the important cultural practices in pest management and is based on the principle of reducing insect pests by increasing the diversity of an ecosystem. On—farm experiments were conducted in villages of semi—arid tropical (SAT) India to identify the appropriate combination of castor (Ricinus communis L.) (Malpighiales: Euphorbiaceae) and intercropping in relation to pest incidence. The diversity created by introducing cluster bean, cowpea, black gram, or groundnut as intercrops in castor (1:2 ratio proportions) resulted in reduction of incidence of insect pests, namely semilooper (Achaea janata L.), leaf hopper (Empoasca flavescens Fabricius), and shoot and capsule borer (Conogethes punctiferalis Guenee). A buildup of natural enemies (Microplitis, coccinellids, and spiders) of the major pests of castor was also observed in these intercropping systems and resulted in the reduction of insect pests. Further, these systems were more efficient agronomically and economically, and were thus more profitable than a castor monocrop. PMID:22934569
Bousquet, Yves; Bouchard, Patrice
Abstract All genus-group names listed in the second edition of the catalogue (1833-1836) of Dejean’s beetle collection are recorded. For each new genus-group name the originally included available species are listed and for generic names with at least one available species, the type species and the current status are given. Names available prior to the publication of Dejean’s second catalogue (1833-1836) are listed in an appendix. The following new synonymies are proposed: Cyclonotum Dejean, 1833 (= Dactylosternum Wollaston, 1854) [Hydrophilidae], Hyporhiza Dejean, 1833 (= Rhinaspis Perty, 1830) [Scarabaeidae], Aethales Dejean, 1834 (= Epitragus Latreille, 1802) [Tenebrionidae], Arctylus Dejean, 1834 (= Praocis Eschscholtz, 1829) [Tenebrionidae], Euphron Dejean, 1834 (= Derosphaerus Thomson, 1858) [Tenebrionidae], Hipomelus Dejean, 1834 (= Trachynotus Latreille, 1828) [Tenebrionidae], Pezodontus Dejean, 1834 (= Odontopezus Alluaud, 1889) [Tenebrionidae], Zygocera Dejean, 1835 (= Disternopsis Breuning, 1939) [Cerambycidae], and Physonota Chevrolat, 1836 (= Anacassis Spaeth, 1913) [Chrysomelidae]. Heterogaster pilicornis Dejean, 1835 [Cerambycidae] and Labidomera trimaculata Chevrolat, 1836 [Chrysomelidae] are placed for the first time in synonymy with Anisogaster flavicans Deyrolle, 1862 and Chrysomela clivicollis Kirby, 1837 respectively. Type species of the following genus-group taxa are proposed: Sphaeromorphus Dejean, 1833 (Sphaeromorphus humeralis Erichson, 1843) [Scarabaeidae], Adelphus Dejean, 1834 (Helops marginatus Fabricius, 1792) [Tenebrionidae], Cyrtoderes Dejean, 1834 (Tenebrio cristatus DeGeer, 1778) [Tenebrionidae], Selenepistoma Dejean, 1834 (Opatrum acutum Wiedemann, 1823) [Tenebrionidae], Charactus Dejean, 1833 (Lycus limbatus Fabricius, 1801) [Lycidae], Corynomalus Chevrolat, 1836 (Eumorphus limbatus Olivier, 1808) [Endomychidae], Hebecerus Dejean, 1835 (Acanthocinus marginicollis Boisduval, 1835) [Cerambycidae], Pterostenus Dejean, 1835
Smith, Andrew B T
Five new species of Trigonopeltastes Burmeister and Schaum, 1840 are described: Trigonopeltastes arborfloricola sp. n. from Nicaragua, Trigonopeltastes formidulosus sp. n. from Costa Rica, Trigonopeltastes henryi sp. n. from Costa Rica, Trigonopeltastes mombachoensis sp. n. from Nicaragua, and Trigonopeltastes warneri sp. n. from Belize and Guatemala. An updated key to species of Trigonopeltastes is presented. Trigonopeltastes nigrinus Bates, 1889 and Trigonopeltastes carus Bates, 1889 are placed in synonymy with Trigonopeltastes geometricus Schaum, 1841, syn. n.. The males of Trigonopeltastes aurovelutinus Curoe, 2011 and Trigonopeltastes simplex Bates, 1889 are described for the first time. New country records are given for the following: Giesbertiolus ornatus Howden, 1988: Costa Rica; Paragnorimus sambucus Howden, 1970: Guatemala; Trichiotinus bibens (Fabricius, 1775): Canada; Trigonopeltastes archimedes Schaum, 1841: Guatemala and Costa Rica; Trigonopeltastes frontalis Bates, 1889: Belize, Guatemala, and Honduras; Trigonopeltastes glabellus Howden, 1988: Guatemala; Trigonopeltastes geometricus Schaum, 1841: Honduras; Trigonopeltastes sallaei sallaei Bates, 1889: Guatemala and Honduras; Trigonopeltastes simplex Bates, 1889: Mexico; Trigonopeltastes variabilis Howden, 1968: Honduras.
Waringer, Johann; Graf, Wolfram; Malicky, Hans
The paper gives a description of the hitherto unknown larva of Psilopteryx psorosa (Kolenati 1860), subspecies bohemosaxonica Mey & Botosaneanu 1985 (Trichoptera: Limnephilidae: Limnephilini, Chaetopterygina; Vshivkova et al. 2007). Information on the morphology of the larva is given and the most important diagnostic features are illustrated. In the context of already available keys, the larva of P psorosa bohemosaxonica keys together with Pseudopsilopteryx zimmeri (McLachlan 1876), Chaetopteryxfusca Brauer 1857 and C. villosa (Fabricius 1798). Psilopteryxpsorosa is not yet separable from P zimmeri but may be easily separated from the two Chaetopteryx species by the median fusion of setal groups sal at the first abdominal sternum in P psorosa which is lacking in C. fusca and C. villosa. With respect to distribution, P. psorosa bohemosaxonica is present in the Bohemian Forest and the Erzgebirge (Upper Austria, Czech Republic, and Germany). In addition, ecological characteristics are briefly discussed.
Mawdsley, Jonathan R.; Erwin, Terry L.; Sithole, Hendrik; Mawdsley, James L.; Mawdsley, Alice S.
Abstract A key is presented for the identification of the four species of Anthia Weber (Coleoptera: Carabidae) recorded from the Republic of South Africa: Anthia cinctipennis Lequien, Anthia circumscripta Klug, Anthia maxillosa (Fabricius), and Anthia thoracica (Thunberg). For each of these species, illustrations are provided of adult beetles of both sexes as well as illustrations of male reproductive structures, morphological redescriptions, discussions of morphological variation, annual activity histograms, and maps of occurrence localities in the Republic of South Africa. Maps of occurrence localities for these species are compared against ecoregional and vegetation maps of southern Africa; each species of Anthia shows a different pattern of occupancy across the suite of ecoregions and vegetation types in the Republic of South Africa. Information about predatory and foraging behaviors, Müllerian mimicry, and small-scale vegetation community associations is presented for Anthia thoracica based on field and laboratory studies in Kruger National Park, South Africa. PMID:22144866
Liu, Shao-Pan; Pan, Zhao; Ren, Guo-Dong
Three species that belong to the genus Epicauta (Coleopera: Meloidae), E. chinensis, E. dubia, and E. sibirica, appear morphologically indistinguishable. The present study aims to resolve the taxonomic status and the relationships among these three species. Identifying adult morphological characters among the three species were compared and illustrated and partial fragments of the mitochondrial gene (COI) for 77 samples, representing seven meloid species, were obtained and analyzed. Analyses of nucleotide composition, genetic distances and phylogenetics were performed. The results of the morphological studies and molecular analyses showed concordance, indicating that the three species are closely related and indistinguishable from one another. Consequently, two new synonyms are proposed: E. chinensis (Laporte, 1840) syn. n. = E. sibirica (Pallas, 1773) and E. dubia (Fabricius, 1781) syn. n. = E. sibirica (Pallas, 1773).
Pinkerton, Marie E; Wellehan, James F X; Johnson, April J; Childress, April L; Fitzgerald, Scott D; Kinsel, Michael J
We report two separate naturally occurring cases of fatal herpesviral disease in Cooper's Hawks (Accipiter cooperii). Gross lesions included splenomegaly and hepatomegaly, with diffuse pale mottling or scattered small white foci. Histologic lesions included splenic and hepatic necrosis associated with eosinophilic intranuclear inclusion bodies characteristic of herpesvirus. In one case, necrosis and inclusions were also noted in bone marrow, thymus, bursa of Fabricius, thyroid gland, parathyroid gland, ceca, and the enteric system. Transmission electron microscopy demonstrated viral particles typical of herpesvirus within hepatocyte nuclei and budding from the nuclear membrane. Herpesviral DNA was amplified via polymerase chain reaction (PCR) of paraffin-embedded liver and spleen, and sequence data were consistent with columbid herpesvirus-1, an alphaherpesvirus of Rock Pigeons (Columba livia). PCR results provide evidence that this disease is transmitted to raptors via Rock Pigeons, most likely through ingestion of Rock Pigeons as prey.
Webster, Reginald P.; Sweeney, Jon D.; DeMerchant, Ian; Turgeon, Martin
Abstract We report ten new species records for the Coleoptera fauna of New Brunswick, Canada from the families Dermestidae, Endecatomidae, Bostrichidae, and Ptinidae. Anthrenus fuscus Olivier and Anthrenus museorum (Linnaeus) of the family Dermestidae are newly recorded for New Brunswick. Endecatomus rugosus (Randall) and the family Endecatomidae are recorded for the first time for New Brunswick and the Maritime provinces. Two Bostrichidae, the adventive Dinoderus minutus (Fabricius) and the native Stephanopachys substriatus (Paykull), are newly recorded for the province. Five species of Ptinidae, the adventive Anobium punctatum (DeGeer) and Microbregma emarginatum emarginatum (Duftschmid), and the native Hadrobregmus notatus (Say), Ptilinus lobatus Casey, and Ptilinus ruficornis Say are added to the faunal list of New Brunswick. Collection data, habitat data, and distribution maps are presented for all these species. PMID:22539890
Webster, Reginald P; Sweeney, Jon D; Demerchant, Ian; Turgeon, Martin
We report ten new species records for the Coleoptera fauna of New Brunswick, Canada from the families Dermestidae, Endecatomidae, Bostrichidae, and Ptinidae. Anthrenus fuscus Olivier and Anthrenus museorum (Linnaeus) of the family Dermestidae are newly recorded for New Brunswick. Endecatomus rugosus (Randall) and the family Endecatomidae are recorded for the first time for New Brunswick and the Maritime provinces. Two Bostrichidae, the adventive Dinoderus minutus (Fabricius) and the native Stephanopachys substriatus (Paykull), are newly recorded for the province. Five species of Ptinidae, the adventive Anobium punctatum (DeGeer) and Microbregma emarginatum emarginatum (Duftschmid), and the native Hadrobregmus notatus (Say), Ptilinus lobatus Casey, and Ptilinus ruficornis Say are added to the faunal list of New Brunswick. Collection data, habitat data, and distribution maps are presented for all these species.
Anisoscelis currently comprises nine species and has been recorded from Texas (here recorded for the first time) on the south of the United States of North America across Mexico, Central and South America, but absent in Chile and the Great and Lesser Antilles; Anisoscelis (Bitta) flavolineatus Blanchard, 1849 is placed in synonym under Anisoscelis (Bitta) alipes Guérin-Méneville, 1831, and Anisoscelis (Bitta) flavolineatus osunai Brailovsky and Mayorga, 1995 is placed in synonymy under Anisoscelis (Bitta) hymenipherus Westwood, 1840; Anisoscelis (Anisoscelis) marginellus (Dallas) previously considered a subspecies under Anisoscelis (Anisoscelis) foliaceus (Fabricius) is removed to specific rank; a new species A. (Bitta) luridus from México is described. Photographs of dorsal habitus and parameres and key to the known species are included. New distributional records for most of the species are given; food plants records are included with an apparent trophic preferences for member of the genus Passiflora. A complete checklist, with subgeneric position of each species is given.
How, Yee-Fatt; Lee, Chow-Yang
A total of 54 bed bug-infested sites (hotels, public accommodations, and residential premises) in Malaysia and Singapore was surveyed between July, 2005 and December, 2008. Only one species of bed bug was found, the tropical bed bug Cimex hemipterus (Fabricius). Bed bug infestations were common in hotels and public accommodations when compared to residential premises. The three most common locations of infestation within an infested premise were the bedding (31.1%), the headboard (30.3%), and cracks and crevices surrounding the baseboard, wall, or floor (23.5%). We speculate that the route of movement of bed bugs in hotels and public accommodations is more direct than in residential premises.
Sharma, J M; Karaca, K; Pertile, T
Reovirus and infectious bursal disease virus are among the naturally occurring viruses that cause immunosuppression in chickens. Both viruses cause necrotic lesions in the bursa of Fabricius and may destroy B cells. This may explain their ability to cause humoral immune suppression. The mechanism(s) of virus-induced suppression of cellular immunity is not well understood. Both viruses inhibit the mitogenic response of T cells in chickens. We have noted that this inhibition may be mediated by inhibitory cytokines such as transforming growth factor-beta and nitric oxide produced by activated macrophages. Preliminary studies have indicated that pretreatment of chickens with an immunomodulator, acemannan, reduced the reovirus-induced inhibition of T cells.
Mawdsley, Jonathan R; Erwin, Terry L; Sithole, Hendrik; Mawdsley, James L; Mawdsley, Alice S
A key is presented for the identification of the four species of Anthia Weber (Coleoptera: Carabidae) recorded from the Republic of South Africa: Anthia cinctipennis Lequien, Anthia circumscripta Klug, Anthia maxillosa (Fabricius), and Anthia thoracica (Thunberg). For each of these species, illustrations are provided of adult beetles of both sexes as well as illustrations of male reproductive structures, morphological redescriptions, discussions of morphological variation, annual activity histograms, and maps of occurrence localities in the Republic of South Africa. Maps of occurrence localities for these species are compared against ecoregional and vegetation maps of southern Africa; each species of Anthia shows a different pattern of occupancy across the suite of ecoregions and vegetation types in the Republic of South Africa. Information about predatory and foraging behaviors, Müllerian mimicry, and small-scale vegetation community associations is presented for Anthia thoracica based on field and laboratory studies in Kruger National Park, South Africa.
Nattier, Romain; Jourdan, Hervé; Mille, Christian; Chazeau, Jean
We present an updated checklist of the ladybird beetle fauna of New Caledonia. Fifty species have been tracked from literature and collections, but six should be removed from the list as they represent false records, invalid or unestablished species: Coccinella boletifera Fauvel, Harmonia conformis (Boisduval), Menochilus duodecimpunctatus (Fauvel), Micraspis lineola (Fabricius), Orcus australasiae Boisduval, and Curinus coeruleus (Mulsant). After our investigations, the current described ladybird beetle fauna totals 44 named species, belonging to 18 valid genera. The endemism rate is 47.7% (21 species), with one endemic subgenus, Scymnus (Caledonus). Based on comparisons of the coccinellid faunas of surrounding regions, the New Caledonian fauna has affinities with Australia and Papua New Guinea more than with the rest of the Pacific area. At least 19 species (43.2%) seem to have been introduced by human activities (either deliberately or accidentally).
Silva, R B; Cruz, I; Zanuncio, J C; Figueiredo, M L C; Canevari, G C; Pereira, A G; Serrão, J E
Eriopis connexa (Germar) (Coleoptera: Coccinellidae) occurs in several countries of South America and its mass rearing is important for biological control programmes. This work evaluated biological aspects of E. connexa larva fed on eggs of Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae) and Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) frozen for one day, fresh eggs of Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae), S. frugiperda newly-hatched caterpillars, nymphs of Rhopalosiphum maidis (Fitch) and Schizaphis graminum (Rondani) (Hemiptera: Aphididae). Duration of larva, pupa and larva to adult stages differed among prey offered, whereas the prepupa stage was similar. Larva, pupa, prepupa and larva to adult viabilities were equal or major of 87.5% in all prey, except for larva fed on newly-hatched larvae of S. frugiperda. Eriopis connexa has good adaptation to different prey corroborating its polyphagous feeding habit, which evidences the potential of this natural enemy for controlling corn and sorghum pests.
Wu, Sheng-Jun; Pan, Sai-Kun; Wang, Hong-Bin; Wu, Jin-Hua
In this study, chitooligosaccharides were prepared from cicada slough of Cryptotympana atrata Fabricius by hydrolysis using hydrogen peroxide (H2O2). Factors affecting the hydrolysis of chitosan were investigated and the optimum hydrolysis conditions were as follows: time, 4 h; temperature, 65 °C; amount of H2O2, 2% (v/v); and pH, 5. Under these conditions, the average degree of polymerisation decreased to ~4.5. The Fourier transform infrared spectra and product sugar composition indicate that there were no significant chemical changes in the backbones of the chitosan treated with H2O2.The chitooligosaccharides had high antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli at the concentration of 100mg/mL. Copyright © 2013 Elsevier B.V. All rights reserved.
Rosa, Paolo; Forshage, Mattias; Paukkunen, Juho; Soon, Villu
The interpretation of Linnaeus' name Sphex semiaurata Linnaeus, 1761 has been controversial. After type examinations, we conclude that it is identical with the common Cleptes pallipes Lepeletier, 1806 and thus re-establish the old synonymy: Cleptes semiauratus (Linnaeus, 1761) (=Cleptes pallipes Lepeletier, 1806, syn. reinst.). We have been unable to find an available name for the species with which it has been confused. In order to be able to designate a suitable type specimen, we prefer to describe it as a new species rather than suggest a replacement name: Cleptes striatipleuris Rosa, Forshage, Paukkunen & Soon sp. nov. (=Cleptes semiauratus sensu Lepeletier, 1806, nec Linnaeus, 1761; =C. splendens sensu Linsenmaier 1959, nec Fabricius, 1798).
Myamba, J; Maxwell, C A; Asidi, A; Curtis, C F
When Tanzanian villages were provided with pyrethroid-treated bednets, bedbugs (Cimicidae) disappeared; however, after about 6 years they have re-appeared in these villages. Using a newly devised test-kit, susceptibility tests of bedbugs Cimex hemipterus (Fabricius) from five of these villages showed that there is resistance to permethrin and alphacypermethrin in bedbugs from each of the villages, in contrast to those from five villages without treated nets. Circumstantial evidence indicates that bedbug resistance to pyrethroid insecticides may evolve more readily in villages with incomplete coverage rates of treated bednets, allowing bedbug infestations to become re-established. Bedbugs have not returned to a village where nearly all the beds have been provided with pyrethroid-treated bednets for 14 years.
Jeffery, J; Sulaiman, S; Oothuman, P; Vellayan, S; Zainol-Ariffin, P; Paramaswaran, S; Razak, A; Muslimin, M; Kamil-Ali, O B; Rohela, M; Abdul-Aziz, N M
The following domiciliary cockroaches were collected from restaurants in five zones of Kuala Lumpur Federal Territory, Malaysia using 1L glass beaker traps baited with ground mouse-pellets: Periplaneta americana (Linnaeus) (n = 820), Periplaneta brunnea Burmeister (n = 46), Blattella germanica (Linnaeus) (n = 12504), Supella longipalpa (Fabricius) (n = 321), Symploce pallens Stephens (n = 29) and Neostylopyga rhombifolia (Stoll) (n = 5). The following bacteria were isolated from 10 cockroach specimens: Enterobacter cloacae, Klebsiella pneumoniae ssp. pneumoniae, Klebsiella pneumoniae ssp. rhinoscleromatis and Serratia liquefaciens from 5 B. germanica; Acinetobacter calcoaceticus var. anitratus, Citrobacter diversus/amalonaticus, Escherichia vulneris and K.p. pneumoniae from 3 P. brunnea; and Citrobacter freundii, Enterobacter agglomerans 4, Escherichia adecarboxylate, E. vulneris, K. p. pneumonia, K. p. rhinoscleromatis and Proteus vulgeris from 2 P. americana.
Lee, Young June; Marshall, David C; Mohagan, Alma; Hill, Kathy B R
This paper provides the first faunal checklist for the family Cicadidae (Hemiptera) from Camiguin of Mindanao Province and Dinagat Island in the Philippines, comprising ten species belonging to nine genera. Cryptotympana shillana Lee & Mohagan sp. nov., Orientopsaltria inermis (Stål, 1870), Purana crassinotata Lee, 2015, and Huechys parvula Haupt, 1924 are recorded for the first time from Camiguin. Platypleura dinagatensis Lee sp. nov., Chremistica kyoungheeae Lee, 2010, Dundubia vaginata (Fabricius, 1787), Oncotympana pallidiventris Stål, 1870, and Philipsalta nigrina Lee, Marshall & Hill sp. nov. are newly recorded from Dinagat Island. A new genus Philipsalta Lee, Marshall & Hill gen. nov. is erected. Huechysini Distant, 1905 syn. nov. is synonymized with Cicadettini Buckton, 1889. Information on geographic distributions of the Camiguin and Dinagat species is also provided.
Wu, Li; Liu, Jinxiong; Chen, Pucheng; Jiang, Yongping; Ding, Leilei; Lin, Yuan; Li, Qimeng; He, Xijun; Chen, Qiusheng; Chen, Hualan
In 2010, a novel Tembusu virus (TMUV) that caused a severe decrease in the egg production of ducks was isolated in southeast China. Given the novelty of this duck pathogen, little information is available regarding its pathogenesis. Here, we systematically investigated the replication kinetics of TMUV PTD2010 in adult male and female ducks. We found that PTD2010 was detectable in most of the parenchymatous organs as well as the oviduct and intestinal tract from days 1 to 7 after inoculation. Viral titers were maintained at high levels for at least 9 days in the spleen, kidney, bursa of Fabricius, brain, and ovary. No virus was detected in any of these organs or tissues at 18 days after inoculation. PTD2010, thus, causes systemic infections in male and female ducks; its replication kinetics show similar patterns in most organs, with the exception of the ovaries and testes.
González-Acuña, D; Corvalan, F; Barrientos, C; Doussang, D; Mathieu, C; Nilsson, L; Casanueva, M E; Palma, R L
A total of 1,177 lice of four species were collected from 124 kelp gulls (Larus dominicanus) and 137 lice of the same four species from 60 Franklin's gulls (Larus pipixcan). The louse Saemundssonia lari (O Fabricius) (Phthiraptera: Philopteridae) was the most numerous on both gull species, with infestation rates of 4.9 on kelp gulls and 1.8 on Franklin's gulls. The second most abundant louse was Quadraceps punctatus (Burmeister), with a high infestation rate but low prevalence on kelp gulls; those parameters were much lower among lice from Franklin's gulls. The composition and community structure of the lice were similar on both host species, but not their infestation rates. In addition, the feather mite Zachvatkinia larica Mironov (Acari: Avenzoariidae) is recorded from kelp gulls and Franklin's gulls for the first time, while the gamasid mite Larinyssus sp. is recorded from kelp gulls, also for the first time. The population parameters of all species of ectoparasites are discussed.
Al-Ahmed, Azzam; Shobrak, Mohammed; Nasser, Mohamed G E-D
Knowledge about chewing lice from marine birds of the Red Sea is minimal. Five species of gulls were examined for chewing lice in three different localities of the Saudi Arabian Red Sea coast. Two gull species were examined for lice for the first time (Larus armenicus Buturlin, 1934 and Larus michahellis Naumann, 1840) and their lice represent new host-louse associations. Four species and two subspecies of lice were identified from 159 specimens collected. Actornithophilus piceus lari (Packard, 1870) and Austromenopon transversum (Denny, 1842) (suborder: Amblycera), and Quadraceps punctatus (Burmeister, 1838) and Saemundssonia lari (O. Fabricius, 1780) (suborder: Ischnocera) were recorded for the first time from Saudi Arabia and Red Sea birds. Taxonomic and ecological notes, type hosts, data on specimens examined, collecting localities, an identification key, and photographs of each species and subspecies are given.
Schultheiss, D; Jonas, U
Johannes Schultheiss (Latinized to 'Scultetus') was one of the first academically trained physicians and surgeons in Germany in the 17th century. After his studies at the University of Padua under Adrian van Spieghel and Fabricius ab Aquapendente he became official physician of his home town of Ulm. His remarkable textbook on surgery, the 'Armamentarium chirurgicum' was first published 10 years after his death and passed through many editions and translations all over Europe. This work contains a complete catalogue of surgical instruments, illustrated demonstrations of a variety of operative procedures and 100 case reports. The success of Scultetus' publication was responsible for an improved standard of the education of the nonacademic barber surgeons, who were treating the majority of the population, and was a significant milestone for the development of surgery as an academic speciality. The life and work of Johannes Scultetus, with reference to urologic aspects, is outlined in this article.
Calvo, J R; Rafii-el-Idrissi, M; Pozo, D; Guerrero, J M
This paper reviews the evidence that supports the hypothesis of the existence of specific binding sites for melatonin on immune cells. These binding sites have been described in human blood lymphocytes and granulocytes, and thymus, spleen, and bursa of Fabricius from different rodents and birds. The dissociation constant values of these binding sites are in the 0.1-1 nM range, suggesting that melatonin may play a physiological role in lymphocyte regulation. Moreover, melatonin binding sites appear to be modulated by guanine nucleotides. Therefore, in addition to other mechanisms described for the regulation of immune function by melatonin, a direct mechanism of regulation can be involved via binding of melatonin by immunocompetent cells.
Abhitha, P; Vinod, K V; Sabu, T K
Invasion by large populations of the litter-dwelling darkling beetle Luprops tristis Fabricius (Coleoptera: Tenebrionidae) following the short spell of summer rains during April, and their extended state of dormancy is a regular event in rubber plantation habitats in south-western India. Strong smelling secretions of the beetle cause blisters on skin of human beings. Such secretions appear defensive because they appear to facilitate their avoidance by other predatory organisms. Defensive glands in the larvae and adults of L. tristis are described, as well as the mode of eversion of the glands. The glands in larvae consist of two pairs of noneversible glands in a conical depression on the 2(nd) and 3(rd) sternites, whereas in adults only one pair occurs between 7(th) and 8(th) sternal segments. These glands may be a major reason for avoidance of larvae and adults by their natural enemies and their very high numbers in the litter of rubber plantations.
Zhou, Guang Fang; Liu, Qing Tao; Zhou, Bin; Qiu, Ya Feng; Liu, Xiao Dong; Ma, Zhi Yong; Feng, Xiu Li; Cao, Rui Bing; Chen, Pu Yan
The bursa of Fabricius (BF) is the acknowledged central humoral immune organ in birds. Bursal septpeptide II (BSP-II) is an immunomodulatory bioactive peptide isolated from BF. To understand the effects of BSP-II on immune induction, gene expression profiles of hybridoma cells treated with BSP-II were evaluated. Pathway analysis showed that regulated genes were involved in cytokine-cytokine receptor interactions, T cell receptor signaling pathway, and pathway in cancer. It was observed that BSP-II reduced tumor cells proliferation and stimulated p53 expression. These results indicate potential mechanisms underlying the effects of the humoral immune system on immune induction, including antitumor activities. Our study has provided a novel insight into immunotherapeutic strategies for treating human tumors.
Zuha, R M; Jenarthanan, L X Q; Disney, R H L; Omar, B
In forensic entomology, larval rearing usually includes the presence of biological contaminants including scuttle flies (Diptera: Phoridae). Scuttle flies are recognized as forensically important insects and have been reported causing nuisance and contamination in laboratory environments. This paper reports for the first time the finding of multiple scuttle fly species affecting colonies of third instar larvae of the Oriental latrine blowfly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae), reared indoors at the Forensic Science Simulation Site, Universiti Kebangsaan Malaysia. Adult scuttle flies were discovered inside a rearing container after the emergence of adult C. megacephala., The scuttle fly species are Megaselia scalaris (Loew), M. spiracularis Schmitz and Puliciphora borinquenensis (Wheeler). Notes on the life history and biology of these species are discussed herein.
Lyu, Zhou; Wan, Li-Hua; Yang, Yong-Qiang; Tang, Rui; Xu, Lyu-Zi
Examining the succession pattern of carrion insects on vertebrate carcasses is widely accepted as an effective method to estimate the postmortem interval (PMI) of decayed bodies. Investigation of the community of sarcosaprophagous insects, especially flies and beetles, is the foundation of the succession pattern study. This study aimed to investigate the sarcosaprophagous beetles succession on animal carcasses in the suburban area of southwestern China and to establish a basic catalog for forensic application. The present study was conducted in 2013 in a mountain in Chongqing municipality with modified Schoenly traps. Carcasses of miniature pig were used to simulate human bodies. For most carcasses, five decomposition stages were observed. A total of 2108 adult coleopterans belonging to at least 61 species and 18 families were collected in the study, and most of the specimens occurred at the advanced decay stage. Omosita colon (Linnaeus, 1758), Necrodes nigricornis (Harold, 1875), Necrobia ruficollis (Fabricius, 1775) and Neosilusa ceylonica (Kraatz, 1857) were the dominant species.
Gruner, S. V.; Slone, D.H.; Capinera, J.L.; Turco, M. P.
Chrysomya megacephala (Fabricius) is a forensically important fly that is found throughout the tropics and subtropics. We calculated the accumulated development time and transition points for each life stage from eclosion to adult emergence at five constant temperatures: 15, 20, 25, 30, and 35 °C. For each transition, the 10th, 50th, and 90th percentiles were calculated with a logistic linear model. The mean transition times and % survivorship were determined directly from the raw laboratory data. Development times of C. megacephala were compared with that of two other closely related species, Chrysomya rufifacies (Macquart) and Phormia regina (Meigen). Ambient and larval mass temperatures were collected from field studies conducted from 2001–2004. Field study data indicated that adult fly activity was reduced at lower ambient temperatures, but once a larval mass was established, heat generation occurred. These development times and durations can be used for estimation of a postmortem interval (PMI).
Xiangqun, Yuan; Ke, Gao; Feng, Yuan; Yalin, Zhang
Most species of Parnara and Pelopidas (Hesperiidae) are important pests of rice. In this study, the antennal morphology, types of sensilla, and their distribution of four skipper butterflies, including Parnara guttata (Bremer & Grey), Pa. bada (Moore), Pelopidas mathias (Fabricius) and Pe. agna (Moore), were observed using a scanning electron microscope. Six distinct morphological types of sensilla were found on the antennae of all of these species: sensilla squamiformia, sensilla trichodea, sensilla chaetica, sensilla auricillica, sensilla coeloconica, and Böhm sensilla. The sensilla trichodea are the most abundant sensilla among the four skipper butterflies, and the sensilla auricillica are confirmed on the antennae of butterflies for the second time. In addition, the possible functions of these sensilla are discussed in the light of previously reported lepidopteran insects, which may provide useful information for further study of the function of these antennal sensilla and for related pests control by applying sex pheromones.
El-Hawagry, Magdi S.; Dhafer, Hathal M. Al
Abstract Five bee-fly species (Bombyliidae, Diptera) have been listed in this paper as new to the Kingdom of Saudi Arabia. Four of the recorded species have been identified to the level of species, namely: Bombomyia discoidea (Fabricius, 1794), Spogostylum candidum (Sack, 1909), Exoprosopa linearis Bezzi, 1924, and Exoprosopa minos (Meigen, 1804), while the fifth one only to genus, Desmatoneura sp. The species have been collected from Al-Baha and Asir Provinces in the south-western part of the Kingdom. One of the four identified species, Exoprosopa linearis, has an Afrotropical affinity, and another two, Spogostylum candidum and Bombomyia discoidea, have considerable Afrotropical distributions, and this result agrees to some extent with studies considering these parts of the Arabian Peninsula, including Al-Baha and Asir Provinces, having Afrotropical influences and may be included in the Afrotropical Region rather than in the Palaearctic Region or the Eremic zone. PMID:25878533
Azevedo, Dihego Oliveira; Neves, Clóvis Andrade; Mallet, Jacenir Reis dos Santos; Gonçalves, Teresa Cristina Monte; Zanuncio, José Cola; Serrão, José Eduardo
This work studied the ultrastructure of the midgut cells of Cimex hemipterus Fabricius (Hemiptera: Cimicidae). The midgut of adult insects was analyzed on different days after a bloodmeal, and three anatomical regions with different digestive functions were apparent. In the anterior midgut, the digestive cells had many spherocrystals, lipid inclusions, and glycogen deposits, suggesting a role in water absorption, ion regulation, digestion, and storage of lipids and sugars. The digestive cells in the middle midgut contained secretory granules in the apical cytoplasm, lysosomes, and large amounts of rough endoplasmic reticulum, suggesting that this midgut region was active in digestive processes. The posterior midgut contained digestive cells with secretory vesicles, lysosomes, rough endoplasmic reticulum, and spherocrystals, suggesting digestion and ion/water absorption. Also, there was strong evidence that the posterior midgut may be the major site of nutrient absorption. The hematophagous heteropteran groups share many of these blood digestion mechanisms.
Dathe, Holger H; Proshchalykin, Maxim Yu
The type specimens of the bee genus Hylaeus Fabricius, 1793 described by Ferdinand Morawitz from Asia and deposited in the Zoological Museum of the Moscow State University and in the Zoological Institute, Russian Academy of Sciences St. Petersburg, are critically reviewed. Precise information with illustrations of types for 39 taxa is provided. New synonymy is established for Hylaeus incongruus Förster, 1871 (= H. biareolatus Morawitz, 1876, syn. nov.); H. breviceps Morawitz, 1876 (= H. bivittatus Morawitz, 1876, syn. nov.); H. punctiscapus Morawitz, 1876 (= H. citrinipes Morawitz, 1893, syn. nov.); H. dolichocephalus Morawitz, 1876 (=Prosopis heliaca Warncke, 1992, syn. nov.); H. laticeps Morawitz, 1876 (= H. nigritarsis Morawitz, 1876, syn. nov.); H. medialis Morawitz, 1890 (= H. bimaculatus Chen & Xu, 2013, syn. nov.). Lectotypes are here designated for the following six nominal taxa: Hylaeus citrinipes Morawitz, 1893, H. flavipes Morawitz, 1876, H. ibex Morawitz, 1877, H. punctiventris Morawitz, 1876, H. trisignatus Morawitz, 1876, and H. turanicus Morawitz, 1876.
Mahat, N A; Zafarina, Z; Jayaprakash, P T
The influence of rain and malathion on the initial oviposition as well as development of blowfly species infesting rabbit carcasses decomposing in sunlit and shaded habitats were studied over a period of 1 year in Kelantan, Malaysia. Chrysomya megacephala (Fabricius) was the most dominant species that infested the carcasses, followed by Chrysomya rufifacies (Macquart). In general, rain, depending on its intensity, delayed initial oviposition by 1-2 days and prolonged the pupation period by 1-3 days. The presence of malathion in the carcasses delayed initial oviposition by 1-3 days and prolonged the pupation period by 2-3 days. These findings deserve consideration while estimating postmortem interval since rain is a commonplace occurrence in Malaysia and malathion is one of the common poisons as an agent for choice to commit suicide.
Alitheen, Noorjahan Banu; McClure, Susan Jane; Yeap, Swee Keong; Kristeen-Teo, Ye Wen; Tan, Sheau Wei; McCullagh, Peter
The bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid by well organized epithelium. Whereas lymphocytes within agglomerates were predominantly Bu-1a(+), a majority of those emigrating onto the supporting membrane were Bu-1a(-) and IgM(+). Both agglomerates and emigrant cells expressed activation-induced deaminase with levels increasing after 24 hours. Emigrating cells were actively proliferating at a rate in excess of both the starting cell population and the population of cells remaining in agglomerates. The potential usefulness of this system for investigating the response of bursal tissue to avian Newcastle disease virus (strain AF2240) was examined.
Cervantes, H M; Munger, L L; Ley, D H; Ficken, M D
An infectious bursal disease (IBD)-vaccinated flock of 23,900 broilers, 17 days of age, experienced sudden onset of depression, dermatitis, and mortality. Postmortem examination showed extensive subcutaneous serosanguineous fluid accumulation over the pectoral muscles, discrete hepatic whitish foci, fluid-filled intestines, and small, flaccid bursae of Fabricius. Gram-stained impression smears from the affected areas revealed numerous gram-positive cocci. Aerobic culture of liver and subcutaneous tissue consistently produced heavy growth of penicillin-sensitive Staphyloccus aureus. Histopathologically, subcutaneous tissue showed diffuse hemorrhage and large numbers of gram-positive cocci with severe congestion and hemorrhage of the underlying skeletal muscle. Liver sections showed multiple, randomly scattered areas of acute coagulation necrosis with numerous gram-positive cocci. Bursal lesions were characterized by extensive follicular necrosis and collapse. A diagnosis of staphylococcal gangrenous dermatitis secondary to IBD was made. Mortality returned to preinfection levels within 72 hours after penicillin was added to the drinking water.
Dermacentor reticulatus (Fabricius, 1794), also known as the marsh tick or ornate dog tick is the second most significant vector (next to Ixodes ricinus) of protozoan, rickettsial and viral pathogens in Europe. Until now, only limited information on the distribution of D. reticulatus in Romania is available. A study was conducted on the distribution of D. reticulatus in Romania during 2012-2014. In this study, D. reticulatus was detected in 17 counties, in 14 of which the species was recorded for the first time. Tick activity was evident throughout the year, except during July and August. Additionally, D. reticulatus was recorded for the first time in Romania from wild boar, foxes and humans. These data suggest that this tick species has a broader geographic range and may have more veterinary and medical importance than previously known.
Chen, Yung-Yi; Hsieh, Ming Kun; Tung, Chun-Yu; Wu, Ching Ching; Lin, Tsang Long
The present study was undertaken to determine the kinetics of viral load and immune response in protection against infectious bursal disease virus (IBDV) by DNA vaccination. Chickens were DNA-vaccinated and challenged with IBDV one week after the third vaccination. Tissues were collected at 12 hours postinfection (HPI), 1 day postinfection (DPI), 3, 5, 7 and 10 DPI. The vaccinated chickens had less viral RNA, with delayed appearance and shorter duration in the bursa of Fabricius, spleen, and cecal tonsil than the challenged control chickens. Their ELISA and neutralizing antibody titers were decreased at 12 HPI and significantly lower (P < 0.05) than those in the challenged control chickens at later time points. Their spleen IFNγ expression was up-regulated compared to that in the DNA-vaccinated chickens without IBDV challenge. These results indicate that DNA vaccination confers protection against IBDV challenge by delayed appearance and rapid clearance of the invading viruses.
Liu, Xiao-Dong; Zhou, Bin; Feng, Xiu-Li; Cao, Rui-Bing; Chen, Pu-Yan
The bursa of Fabricius (BF) is the key humoral immune organ unique to birds, and is critical for early B-lymphocyte proliferation and differentiation. However, the molecular basis and mechanisms through which the BF regulates B cell development are not fully understood. In this study, we isolated and identified a new bursal peptide (BP8, AGHTKKAP) by RP-HPLC and MALDI-TOF-MS. BP8 promoted colony-forming pre-B formation, bound B cell precursor, regulated B cell development in vitro as well as in vivo, upstream of the EBF-E2A-Pax5 regulatory complex and increased immunoglobulin secretion. These data revealed a bursal-derived multifunctional factor BP8 as a novel biomaterial which is essential for the development of the immune system. This study elucidates further the mechanisms involved in humoral immune system and has implications in treating human diseases.
Braithwaite, L.A.; Morrison, W.D.; Bate, L.; Otten, L.; Hunter, B.; Pei, D.C. )
Twenty-two 1-wk-old broiler chicks (Gallus domesticus) were housed at 16 C and operantly conditioned to activate either a 250-W infrared bulb (control) or a microwave generator delivering 13 mW/cm2 (treated). Plasma corticosterone concentration did not differ between groups (P greater than .05) at 4 wk of age. At that time the birds were killed, and post-mortem examination revealed no treatment differences in gross morphology of the chicks or in weights of spleen and bursa of Fabricius (P greater than .05). Histological study of comparable segments of spleen, bursa, adrenal, and thyroid tissue did not show differences in any of the chosen parameters (P greater than .05). Heterophil:lymphocyte ratios, packed cell volume, and total plasma protein content were similar between groups (P greater than .05). These results suggest that operant exposure to low density microwave radiation did not result in stress or immunological disturbances.
Zhang, Dong; Li, Xinyu; Liu, Xianhui; Wang, Qike; Pape, Thomas
Antennae are among the most elaborate sensory organs in adult flies, and they provide rich information for phylogenic studies. The antennae of five out of eight species of Gasterophilus Leach (G. haemorrhoidalis (Linnaeus), G. intestinalis (De Geer), G. nasalis (Linnaeus), G. nigricornis (Loew) and G. pecorum (Fabricius)), were examined using scanning electron microscopy. The general morphology, including distribution, type, size, and ultrastructure of antennal sensilla were presented, and the definition of auriculate sensilla and sensory pits were updated and clarified. Eighteen antennal characters were selected to construct the first species-level phylogeny of this genus. The monophyly of Gasterophilus was supported by the presence of coeloconic sensilla III on the antennal arista. The species-level cladogram showed G. pecorum branching off at the base, and the remaining species forming the topology (G. intestinalis+ (G. haemorrhoidalis+ (G. nasalis+ G. nigricornis))). Our research shows the importance of the antennal ultrastructure as a reliable source for phylogenetic analysis. PMID:27703229
Guy, J S; Ficken, M D; Barnes, H J; Wages, D P; Smith, L G
Depression, somnolence, and increased mortality were observed in 2-week-old turkeys inoculated intramuscularly with either eastern equine encephalitis (EEE) virus or Highlands J (HJ) virus. Mortality rates in EEE virus- and HJ virus-inoculated turkeys were 7/30 (23%) and 9/30 (27%), respectively; no sham-inoculated controls died. Both EEE virus- and HJ virus-inoculated turkeys developed viremia that lasted 2 days; peak mean titers were 5.5 and 3.2 log10 plaque-forming units per ml of blood, respectively. Pathologic changes in both EEE virus- and HJ virus-inoculated turkeys consisted primarily of multifocal necrosis in the heart, kidney, and pancreas, and lymphoid necrosis and depletion in the thymus, spleen, and bursa of Fabricius. The findings indicate that EEE virus and HJ virus are pathogenic for young turkeys.
Bermudez, A J; Ley, D H; Levy, M G; Ficken, M D; Guy, J S; Gerig, T M
Cryptosporidium meleagridis oocysts, originally isolated from droppings of commercial turkey poults with increased mortality due to viral (reovirus) hepatitis and enteritis, were treated with peracetic acid to kill companion bacteria and viruses and then propagated by passage in young turkeys. Thirty-eight 5-day-old large white turkey poults were inoculated by crop gavage with 500,000 cryptosporidial oocysts and compared with 40 uninoculated poults. Cryptosporidial oocysts shedding began 3 days postinoculation (PI), peaked on day 4 PI, and persisted at a low level for the duration of the 21-day trial. Low to moderate cryptosporidial infections of the ileal mucosa (days 3, 6, and 15 PI), cecal mucosa (days 3, 6, and 21 PI), and bursa of Fabricius (days 6, 12, 15 and 21 PI) were found on histopathological examination. There were no differences in mean body weights between the inoculated and uninoculated groups, and no mortality or clinical signs of disease were seen in either group.
Abhitha, P.; Vinod, K.V; Sabu, T.K.
Invasion by large populations of the litter-dwelling darkling beetle Luprops tristis Fabricius (Coleoptera: Tenebrionidae) following the short spell of summer rains during April, and their extended state of dormancy is a regular event in rubber plantation habitats in south-western India. Strong smelling secretions of the beetle cause blisters on skin of human beings. Such secretions appear defensive because they appear to facilitate their avoidance by other predatory organisms. Defensive glands in the larvae and adults of L. tristis are described, as well as the mode of eversion of the glands. The glands in larvae consist of two pairs of noneversible glands in a conical depression on the 2nd and 3rd sternites, whereas in adults only one pair occurs between 7th and 8th sternal segments. These glands may be a major reason for avoidance of larvae and adults by their natural enemies and their very high numbers in the litter of rubber plantations. PMID:20569139
Webster, Reginald P; Sweeney, Jon D; Demerchant, Ian; Bouchard, Patrice; Bousquet, Yves
Thirteen species of Tenebrionidae are newly reported for New Brunswick, Canada. Paratenetus punctatus Spinola, Pseudocistela brevis (Say), Mycetochara foveata (LeConte), and Xylopinus aenescens LeConte are recorded for the first time from the Maritime provinces. Platydema excavatum (Say) is removed from the faunal list of New Brunswick, and the presence of Platydema americanum Laporte and Brullé for the province is confirmed. This brings the total number of species of Tenebrionidae known from New Brunswick to 42. Two species of Zopheridae, Bitoma crenata Fabricius and Synchita fuliginosa Melsheimer, are newly recorded for New Brunswick, bringing the number of species known from the province to four. Bitoma crenata is new to the Maritime provinces. Collection and habitat data are presented for these species.
Perissinotto, Renzo; Šípek, Petr; Ball, Jonathan B.
Abstract A new high altitude montane species of Trichostetha Burmeister, 1842 is described from the Elandsberg range of the Western Cape interior. This represents the 14th species of the genus and the first to be reported with a description of its larva. It is a significant addition to the growing number of species that exhibit no adult feeding behaviour and a short period of activity restricted to the onset of summer. Larvae dwell in rock crevices, feeding on decomposing plant matter. The genus Trichostetha is heterogeneous and the complex variability observed in some species, especially T. capensis (Linnaeus, 1767), requires the re-instatement of taxa that were recently synonymised. Thus, T. bicolor Péringuey, 1907 is here re-proposed as a separate species and T. capensis hottentotta (Gory & Percheron, 1833) as a separate subspecies. Conversely, T. alutacea Allard, 1994 is recognised as a dark variety of T. signata (Fabricius, 1775) and is, consequently, synonymised with this species. PMID:25161367
Santin, Elizabeth; Paulillo, Antonio C; Maiorka, Paulo C; Alessi, Antonio C; Krabbe, Everton L; Maiorka, Alex
This study aimed to evaluate the effect of dietary ochratoxin, in the presence or absence of aluminosilicate, on the histology of the bursa of Fabricius, liver and kidneys, and on the humoral immune response of broilers vaccinated against Newcastle disease virus. The exposure of birds to 2 p.p.m. ochratoxin, in the presence or absence of aluminosilicate, reduced their humoral immune response and the number of mitotic cells in the bursa. The relative weight of the livers of the birds exposed to this toxin was increased and, microscopically, there was hepatocyte vacuolation and megalocytosis with accompanying hyperplasia of the biliary epithelium. The kidneys showed hypertrophy of the renal proximal tubular epithelium, with thickening of the glomerular basement membrane. Aluminosilicate did not ameliorate the deleterious effects of the ochratoxin.
David, K J; Hancock, D L; Freidberg, A; Goodger, K F M
Seven new species of Euphranta Loew are described, viz., Euphranta dysoxyli David, sp. nov., E. hyalipennis David & Freidberg, sp. nov, E. diffusa David, sp. nov., E. haldwanica Hancock & Goodger, sp. nov. and E. thandikudi David, sp. nov. from India; E. neochrysopila David, Freidberg, Hancock & Goodger, sp. nov. from Sri Lanka and E. ridleyi Hancock & Goodger, sp. nov. from Singapore. Notes are provided on the identities of E. corticicola (Hering) and E. klugii (Wiedemann); E. signatifacies Hardy is synonymised with E. klugii; E. dissoluta (Bezzi) and E. burtoni Hardy are synonymised with E. crux (Fabricius) and Dimeringophrys parilis (Hardy) is reinstated as a valid species. Coelotrypes latilimbatus (Enderlein), Dimeringophrys pallidipennis Hardy, D. parilis (Hardy) and Hardyadrama excoecariae Lee are newly recorded from India, E. crux from Sri Lanka and E. klugii from Indonesia. An unnamed species of Coelopacidia Enderlein from India is illustrated. An illustrated key to species of Euphranta Loew from India is also provided.
A new subfamily, Bothriocrotoninae n. subfam., for the genus Bothriocroton Keirans, King & Sharrad, 1994 status amend. (Ixodida: Ixodidae), and the synonymy of Aponomma Neumann, 1899 with Amblyomma Koch, 1844.
Klompen, Hans; Dobson, Susan J; Barker, Stephen C
Evidence suggesting polyphyly of the traditionally recognised tick genus Aponomma Neumann, 1899 is summarized. Continued recognition of this genus in its current concept leaves a polyphyletic genus Aponomma and a paraphyletic genus Amblyomma Koch, 1844. To improve the correlation between our understanding of phylogenetic relationships in metastriate ticks and their classification, a few changes in classification are proposed. The members of the 'indigenous Australian Aponomma' group (sensu Kaufman, 1972), A. auruginans Schulze, 1936, A. concolor Neumann, 1899, A. glebopalma Keirans, King & Sharrad, 1994, A. hydrosauri (Denny, 1843) and A. undatum (Fabricius, 1775), are transferred to Bothriocroton Keirans, King & Sharrad, 1994, which is raised to full generic rank. The remaining members of Aponomma are transferred to Amblyomma. Uncertainty remains on relationships of Bothriocroton to other metastriate lineages and on the systematic position of the two species formerly included in the 'primitive Aponomma' group, A. elaphense Price, 1959 and A. sphenodonti Dumbleton, 1943.
Thirty six species of horse flies (Tabanidae) were previously known from Serbia (Europe). The present faunistic study of horse flies (Tabanidae) has resulted in the recording of the 4 new species Atylotus fulvus (Meigen, 1804); Tabanus miki Brauer in Brauer and Bergenstamm, 1880; Tabanus unifasciatus Loew, 1858; and Heptatoma pellucens (Fabricius, 1776), in the fauna of Serbia. The genus Heptatoma Meigen, 1803 is cited for the first time in the fauna of Serbia. 40 species are currently known from Serbia, belonging to nine genera. The fauna can be considered relatively poorly studied. Most of the species belong to the Boreal-Eurasian type of fauna 23, followed by the South European group with 8 species, the Mediterranean group with 6 species, European group with 2 species and Central European group with 1 species.
Moura, Debora C; Schlindwein, Clemens
Euglossini are typical bees of Neotropical rainforests and only a few species occur in the Caatinga. The São Francisco river, which is the only permanent river in the semi-arid NE-Brazil, is bordered by a gallery forest with evergreen leaves. This environment offers flooral rewards along the year. Surveys of euglossine bees by attracting males to scent baits showed that species of the Atlantic Rainforest like Euglossa imperialis Cockerel, E. truncata Moure and Eulaema cingulata Fabricius occur in the gallery forest of the São Francisco river under the semi-arid climate of the caatinga region. These bees are restricted to the gallery forests which function as bio-corridors, and are absent at places where the forests were cut down. This emphasizes the need to protect the threatened gallery forests to maintain biodiversity.
Webster, Reginald P.; Sweeney, Jon D.; DeMerchant, Ian
Abstract Two species of Sphindidae, Odontosphindus denticollis LeConteand Sphindus trinifer Casey, are reported for the first time for New Brunswick. Another species, Sphindus near americanus LeConte is reported from the province but may be an undescribed species, pending further study. Five species of Erotylidae are newly recorded for the province, including Tritoma humeralis Fabricius and Tritoma sanguinipennis (Say), which are new to the Maritime provinces. Three species of Monotomidae are added to the New Brunswick faunal list, including Pycnotomina cavicollis (Horn), which is newly recorded for the Maritime provinces. Six additional species of Cryptophagidae are reported for the province and the presence of Antherophagus convexulus LeContein New Brunswick is confirmed. Cryptophagus pilosus Gyllenhal and Myrmedophila americana (LeConte) are newly reported to the Maritime provinces. PMID:22539893
Belmain, Steven R; Amoah, Barbara A; Nyirenda, Stephen P; Kamanula, John F; Stevenson, Philip C
Tephrosia vogelii has been used for generations as a pest control material in Africa. Recently, two chemotypes have been reported based on the occurrence (chemotype 1) or absence (chemotype 2) of rotenoids. This could have an impact on the efficacy and reliability of this material for pest control. We report that chemotype 2 has no pesticidal activity against Callosobruchus maculatus Fabricius (family Chrysomelidae) and that this is associated with the absence of rotenoids. We present a first report of the comparative biological activity of deguelin, tephrosin, α-toxicarol, and sarcolobine and show that not all rotenoids are equally effective. Tephrosin was less toxic than deguelin which was less active than rotenone, while obovatin 5-methyl ether, the major flavonoid in chemotype 2 was inactive. We also report that in chemotype 1 the occurrence of rotenoids shows substantial seasonal variation.
Schuh, J.C.; Sileo, L.; Siegfried, L.M.; Yuill, Thomas M.
Inclusion body disease of cranes was the cause of death in 17 immature and mature cranes of 5 different species in Wisconsin. A herpesvirus of unknown origin was the apparent cause. An isolate of this herpesvirus was used to experimentally infect 3 species of cranes. Macroscopic and microscopic lesions associated with naturally acquired and experimentally induced disease were essentially identical. Multifocal hepatic and splenic necrosis was found in all cranes evaluated. Necrosis of the gastrointestinal tract, thymus, and bursa of Fabricius also was seen in some of the cranes. Eosinophilic intranuclear inclusion bodies often were commonly associated with hepatic lesions, sometimes with the splenic lesions, and rarely with the thymic or gastrointestinal tract lesions. The lesions of this inclusion body disease were similar to those reported for cranes in Austria from which a crane herpesvirus was isolated.
Tsutsui, Y; Maeto, K; Hamaguchi, K; Isaki, Y; Takami, Y; Naito, T; Miura, K
Although apomixis is the most common form of parthenogenesis in diplodiploid arthropods, it is uncommon in the haplodiploid insect order Hymenoptera. We found a new type of spontaneous apomixis in the Hymenoptera, completely lacking meiosis and the expulsion of polar bodies in egg maturation division, on the thelytokous strain of a parasitoid wasp Meteorus pulchricornis (Wesmael) (Braconidae, Euphorinae) on pest lepidopteran larvae Spodoptera litura (Fabricius) (Noctuidae). The absence of the meiotic process was consistent with a non-segregation pattern in the offspring of heterozygous females, and no positive evidence was obtained for the induction of thelytoky by any bacterial symbionts. We discuss the conditions that enable the occurrence of such rare cases of apomictic thelytoky in the Hymenoptera, suggesting the significance of fixed heterosis caused by hybridization or polyploidization, symbiosis with bacterial agents, and occasional sex. Our finding will encourage further genetic studies on parasitoid wasps to use asexual lines more wisely for biological control.
Abstract Thirty six species of horse flies (Tabanidae) were previously known from Serbia (Europe). The present faunistic study of horse flies (Tabanidae) has resulted in the recording of the 4 new species Atylotus fulvus (Meigen, 1804); Tabanus miki Brauer in Brauer and Bergenstamm, 1880; Tabanus unifasciatus Loew, 1858; and Heptatoma pellucens (Fabricius, 1776), in the fauna of Serbia. The genus Heptatoma Meigen, 1803 is cited for the first time in the fauna of Serbia. 40 species are currently known from Serbia, belonging to nine genera. The fauna can be considered relatively poorly studied. Most of the species belong to the Boreal-Eurasian type of fauna 23, followed by the South European group with 8 species, the Mediterranean group with 6 species, European group with 2 species and Central European group with 1 species. PMID:21998507
Ambrose, D. P.; Rajan, S. J.; Raja, J. M.
The impact of the insecticide, Synergy-505 (chlorpyrifos 50% and cypermethrin 5% E.C), on the functional response, predatory behavior, and mating behavior of a non-target reduviid, Rhynocoris marginatus (Fabricius) (Hemiptera: Reduviidae), a potential biological control agent, were studied. Though both normal and Synergy-505-exposed R. marginatus exhibited Holling's type II curvilinear functional response, Synergy-505 caused a less pronounced type II functional response with reduced numbers of prey killed, attack rate, searching time, and prolonged handling time in 4th and 5th nymphal instars and adult males and females reflecting reduced predatory potential. Synergy-505 also delayed the predatory and mating events. The impacts of Synergy-505 on functional response, predatory behavior, and mating behavior were more evident at higher concentrations of Synergy-505. PMID:21265616
Tavares, Marcos; Santana, William
A new genus, Teramnonotus n. gen., is erected for Elamena gordonae Monod, 1956. The new genus includes two new species, T. johnlucasi n. gen., n. sp. and T. monodi n. gen., n. sp., described herein from eastern Australia (Queensland) and the southwestern Atlantic (Brazil), respectively. Teramnonotus n. gen. superficially resembles Elamena H. Milne Edwards, 1837, and Trigonoplax H. Milne Edwards, 1853, of which it can be easily distinguished by a combination of characters: eyes and ocular peduncle, rostrum, carapace, thoracic sternite 8 and thoracic pleurite 8, and the inhalant water openings. The validity of the obscure species Elamena mexicana H. Milne Edwards, 1853, is not supported and it is synonymised with Halicarcinus planatus (Fabricius, 1775).
Ambrose, Dunston P; Lenin, E Arockia; Kiruba, D Angeline
Available mitochondrial DNA sequences viz., 16S, Cyt b, Cyt c oxidase subunit - I, and Cyt c subunit-like - I gene of Rhynocoris (Kolenati) species were subjected to phylogenetic analysis to understand the intrageneric and intraspecific variations and the role of geographical isolation on speciation; using CLUSTAL W in MEGA version 5.1. This analysis includes fifteen species and four ecotypes of R. kumarii Ambrose and Livingstone and three morphs of R. marginatus (Fabricius) from four countries viz., Canada, China, Korea, and South Africa. The pairwise genetic distances were calculated and phylograms were constructed using Maximum Likelihood, Maximum Parsimony, and Neighbor-Joining methods. These preliminary analyses not only demarcated the fifteen species of Rhynocoris, the four ecotypes of R. kumarii, and the three morphs of R. marginatus, but also revealed phylogenetic relationships and the role of geographical isolation and polymorphism on speciation.
Overgaard Nielsen, B.; Funch, P.; Toft, S.
A host invasion strategy hitherto unknown from other insect parasitoids was observed in the dipteran Acrocera orbicula (Fabricius) (Diptera: Acroceridae) parasitizing the wolf spider, Pardosa prativaga (L. Koch) (Araneida: Lycosidae). In laboratory experiments the free-living first instar acrocerid larvae attached themselves firmly to the spiders' integument by the mouthparts, cutting a tiny hole through the integument. No first instar larvae invaded the host. A week later the parasitoids molted, and a small, flexible, and glabrous second instar larva left each of the attached first instar exuviae and invaded the host through the attachment hole of the first instar larva. The novel host invasion pattern observed may reduce physical damage to the host in the initial phase of endoparasitism, enhancing parasitoid survival.
Spitzen, J; van Huis, A
Development and reproductive success of the solitary egg parasitoid Uscana lariophaga Steffan were examined after development in eggs of the bruchid storage pest Callosobruchus maculatus Fabricius reared at either low or high densities on cowpea seeds and laid at day 1 and 4 of maternal life. Both bruchid larval competition and maternal age negatively affected egg size, but the latter more than the former. Uscana lariophaga reared in small hosts developed slower, were smaller and produced fewer eggs compared to parasitoids reared in large hosts. Fecundity of the parasitoid was heavily influenced by host egg size. This was reflected in the values for the intrinsic rate of increase of U. lariophaga, which differed for wasps that developed in host eggs laid by bruchid females of different age. Wasps allocated marginally more female offspring to larger hosts.
Schmitt, Michael; Rönn, Thomas
Abstract A comparison of the geographical distribution patterns of 647 species of Chrysomelidae in Central Europe revealed 13 types of distribution: (1) widely distributed, (2) southern, (3) southeastern, (4) southwestern, (5) northern, (6) eastern, (7) south east quarter, (8) south west quarter, (9) fragmented, (10) montane, (11) subalpine & alpine, (12) scattered, (13) unusual, and irregular patterns produced by insufficient data. Some of these distributions are trivial (e. g. northern, eastern, etc., alpine) but others are surprising. Some cannot be explained, e. g. the remarkable gaps in the distribution of Chrysolina limbata (Fabricius, 1775) and in Aphthona nonstriata (Goeze, 1777). Although our 63.000 records are necessarily tentative, we found that the distribution maps from these data reflect in many cases the common knowledge on the occurrence of leaf beetles in specific areas. PMID:22303107
Dass, Angeline David; Ghani, Idris Abd.
Description of five species of Xanthopimpla Saussure, 1829 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia was done using specimens deposited in Centre for Insects Systematics, Universiti Kebangsaan Malaysia (CIS, UKM). Type and non-type specimens were loaned from several repositories namely Zoological Museum of Amsterdam Netherlands (ZMAN), Swedish Museum of Natural History (NRM), British Natural History Museum London (BMNH) and Department of Agricultural Malaysia (DOA) for identification and comparison. The specimens were identified to the species level which gives rise to five species namely Xanthopimpla conica Cushman, 1925, Xanthopimpla despinosa leipephelis Townes & Chiu, 1970, Xanthopimpla flavolineata Cameron, 1907, Xanthopimpla punctata (Fabricius, 1781) and Xanthopimpla tricapus impressa Townes & Chiu, 1970. A dichotomous key and descriptions for five Xanthopimpla spesies were provided. Photos and illustrations of carina on propodeum were also included in this paper.
Schade, Daynika J; Vamosi, Steven M
Early body condition may be important for adult behavior and fitness, and is impacted by a number of environmental conditions and biotic interactions. Reduced fecundity of adult females exposed to larval competition may be caused by reduced body condition or shifts in relative body composition, yet these mechanisms have not been well researched. Here, body mass, body size, scaled body mass index, and two body components (water content and lean dry mass) of adult Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae: Bruchinae) females exposed to larval competition or reared alone were examined. Experimental females emerged at significantly smaller body mass and body size than control females. Additionally, scaled body mass index and water content, but not lean dry mass, were significantly reduced in experimental females. To our knowledge, these are the first results that demonstrate a potential mechanism for previously documented direct effects of competition on fecundity in female bruchine beetles.
Rao, J R; Sharma, N N; Iyer, P K; Sharma, A K
The clinical signs and gross lesions caused by Eimeria uzura (10(5) oocysts) in Japanese quail (Coturnix coturnix japonica) exhibited little or no influence in the face of intercurrent dietary aflatoxicosis (1 p.p.m. of aflatoxin B1 from Day 0 to 55). Similarly, no significant differences in the mucosal morphology of the intestine were evident histologically between the two groups of Japanese quail. The nervous signs of ataxia, leg weakness, incoordination of movement, torticollis and terminal opisthotonos were toxin-induced manifestations. In the aflatoxic quail, hypoplastic changes and selective depletion of lymphocytes were more prominent in the bursa of fabricius. Increased relative mean weights of liver, kidney, spleen, crop, proventriculus and gizzard were observed in birds due to aflatoxin sensitivity. The combination of E. uzura infection and aflatoxicosis in Japanese quail may cause significant weight loss, and increased oocyst production and reproductive potential.
García-Garza, María Elena; León-González, Jesús Angel De
Abstract The main objective of this work is to contribute to the taxonomic knowledge of the species of Capitellidae reported for the Eastern Tropical Pacific. This catalogue includes the original name of each species, new names, synonymies, type localities, the museum or institution where the type material is deposited, revision of the material reported for the region by different authors, new examined material, previous reports from other regions of the world, and comments on systematics and distributions. The catalogue lists 43 species in 19 genera. Of these, 6 species were erroneously recorded for the region (Decamastus gracilis Hartman, 1963; Decamastus nudus Thomassin, 1970; Mastobranchus variabilis Edwing, 1984; Notomastus aberans Day, 1957; Notomastus americanus Day, 1973; Notomastus latericeus Sars, 1851) and 5 species are found here to be questionable records for the Eastern Tropical Pacific (Capitella capitata (Fabricius, 1780); Dasybranchus glabrus Moore, 1909; Decamastus lumbricoides Grube, 1878; Notomastus lineatus Claparède, 1870 and Notomastus tenuis Moore, 1909). PMID:22368451