Accuracy of coded excitation methods for measuring the time of flight: Application to ultrasonic characterization of wood samples.

PubMed

Lasaygues, Philippe; Arciniegas, Andres; Espinosa, Luis; Prieto, Flavio; Brancheriau, Loïc

2018-05-26

Ultrasound computed tomography (USCT) using the transmission mode is a way to detect and assess the extent of decay in wood structures. The resolution of the ultrasonic image is closely related to the different anatomical features of wood. The complexity of the wave propagation process generates complex signals consisting of several wave packets with different signatures. Wave paths, depth dependencies, wave velocities or attenuations are often difficult to interpret. For this kind of assessment, the focus is generally on signal pre-processing. Several approaches have been used so far including filtering, spectrum analysis and a method involving deconvolution using a characteristic transfer function of the experimental device. However, all these approaches may be too sophisticated and/or unstable. The alternative methods proposed in this work are based on coded excitation, which makes it possible to process both local and general information available such as frequency and time parameters. Coded excitation is based on the filtering of the transmitted signal using a suitable electric input signal. The aim of the present study was to compare two coded-excitation methods, a chirp- and a wavelet-coded excitation method, to determine the time of flight of the ultrasonic wave, and to investigate the feasibility, the robustness and the precision of the measurement of geometrical and acoustical properties in laboratory conditions. To obtain control experimental data, the two methods were compared with the conventional ultrasonic pulse method. Experiments were conducted on a polyurethane resin sample and two samples of different wood species using two 500 kHz-transducers. The relative errors in the measurement of thickness compared with the results of caliper measurements ranged from 0.13% minimum for the wavelet-coded excitation method to 2.3% maximum for the chirp-coded excitation method. For the relative errors in the measurement of ultrasonic wave velocity, the coded excitation methods showed differences ranging from 0.24% minimum for the wavelet-coded excitation method to 2.62% maximum for the chirp-coded excitation method. Methods based on coded excitation algorithms thus enable accurate measurements of thickness and ultrasonic wave velocity in samples of wood species. Copyright © 2018 Elsevier B.V. All rights reserved.

Ultrasound strain imaging using Barker code

NASA Astrophysics Data System (ADS)

Peng, Hui; Tie, Juhong; Guo, Dequan

2017-01-01

Ultrasound strain imaging is showing promise as a new way of imaging soft tissue elasticity in order to help clinicians detect lesions or cancers in tissues. In this paper, Barker code is applied to strain imaging to improve its quality. Barker code as a coded excitation signal can be used to improve the echo signal-to-noise ratio (eSNR) in ultrasound imaging system. For the Baker code of length 13, the sidelobe level of the matched filter output is -22dB, which is unacceptable for ultrasound strain imaging, because high sidelobe level will cause high decorrelation noise. Instead of using the conventional matched filter, we use the Wiener filter to decode the Barker-coded echo signal to suppress the range sidelobes. We also compare the performance of Barker code and the conventional short pulse in simulation method. The simulation results demonstrate that the performance of the Wiener filter is much better than the matched filter, and Baker code achieves higher elastographic signal-to-noise ratio (SNRe) than the short pulse in low eSNR or great depth conditions due to the increased eSNR with it.

Ultrasound Transducer and System for Real-Time Simultaneous Therapy and Diagnosis for Noninvasive Surgery of Prostate Tissue

PubMed Central

Jeong, Jong Seob; Chang, Jin Ho; Shung, K. Kirk

2009-01-01

For noninvasive treatment of prostate tissue using high intensity focused ultrasound (HIFU), this paper proposes a design of an integrated multi-functional confocal phased array (IMCPA) and a strategy to perform both imaging and therapy simultaneously with this array. IMCPA is composed of triple-row phased arrays: a 6 MHz array in the center row for imaging and two 4 MHz arrays in the outer rows for therapy. Different types of piezoelectric materials and stack configurations may be employed to maximize their respective functionalities, i.e., therapy and imaging. Fabrication complexity of IMCPA may be reduced by assembling already constructed arrays. In IMCPA, reflected therapeutic signals may corrupt the quality of imaging signals received by the center row array. This problem can be overcome by implementing a coded excitation approach and/or a notch filter when B-mode images are formed during therapy. The 13-bit Barker code, which is a binary code with unique autocorrelation properties, is preferred for implementing coded excitation, although other codes may also be used. From both Field II simulation and experimental results, whether these remedial approaches would make it feasible to simultaneously carry out imaging and therapy by IMCPA was verifeid. The results showed that the 13-bit Barker code with 3 cycles per bit provided acceptable performances. The measured −6 dB and −20 dB range mainlobe widths were 0.52 mm and 0.91 mm, respectively, and a range sidelobe level was measured to be −48 dB regardless of whether a notch filter was used. The 13-bit Barker code with 2 cycles per bit yielded −6dB and −20dB range mainlobe widths of 0.39 mm and 0.67 mm. Its range sidelobe level was found to be −40 dB after notch filtering. These results indicate the feasibility of the proposed transducer design and system for real-time imaging during therapy. PMID:19811994

Ultrasound transducer and system for real-time simultaneous therapy and diagnosis for noninvasive surgery of prostate tissue.

PubMed

Jeong, Jong Seob; Chang, Jin Ho; Shung, K Kirk

2009-09-01

For noninvasive treatment of prostate tissue using high-intensity focused ultrasound this paper proposes a design of an integrated multifunctional confocal phased array (IMCPA) and a strategy to perform both imaging and therapy simultaneously with this array. IMCPA is composed of triple-row phased arrays: a 6-MHz array in the center row for imaging and two 4-MHz arrays in the outer rows for therapy. Different types of piezoelectric materials and stack configurations may be employed to maximize their respective functionalities, i.e., therapy and imaging. Fabrication complexity of IMCPA may be reduced by assembling already constructed arrays. In IMCPA, reflected therapeutic signals may corrupt the quality of imaging signals received by the center-row array. This problem can be overcome by implementing a coded excitation approach and/or a notch filter when B-mode images are formed during therapy. The 13-bit Barker code, which is a binary code with unique autocorrelation properties, is preferred for implementing coded excitation, although other codes may also be used. From both Field II simulation and experimental results, we verified whether these remedial approaches would make it feasible to simultaneously carry out imaging and therapy by IMCPA. The results showed that the 13-bit Barker code with 3 cycles per bit provided acceptable performances. The measured -6 dB and -20 dB range mainlobe widths were 0.52 mm and 0.91 mm, respectively, and a range sidelobe level was measured to be -48 dB regardless of whether a notch filter was used. The 13-bit Barker code with 2 cycles per bit yielded -6 dB and -20 dB range mainlobe widths of 0.39 mm and 0.67 mm. Its range sidelobe level was found to be -40 dB after notch filtering. These results indicate the feasibility of the proposed transducer design and system for real-time imaging during therapy.

Feasibility of coded vibration in a vibro-ultrasound system for tissue elasticity measurement.

PubMed

Zhao, Jinxin; Wang, Yuanyuan; Yu, Jinhua; Li, Tianjie; Zheng, Yong-Ping

2016-07-01

The ability of various methods for elasticity measurement and imaging is hampered by the vibration amplitude on biological tissues. Based on the inference that coded excitation will improve the performance of the cross-correlation function of the tissue displacement waves, the idea of exerting encoded external vibration on tested samples for measuring its elasticity is proposed. It was implemented by integrating a programmable vibration generation function into a customized vibro-ultrasound system to generate Barker coded vibration for elasticity measurement. Experiments were conducted on silicone phantoms and porcine muscles. The results showed that coded excitation of the vibration enhanced the accuracy and robustness of the elasticity measurement especially in low signal-to-noise ratio scenarios. In the phantom study, the measured shear modulus values with coded vibration had an R(2 )= 0.993 linear correlation to that of referenced indentation, while for single-cycle pulse the R(2) decreased to 0.987. In porcine muscle study, the coded vibration also obtained a shear modulus value which is more accurate than the single-cycle pulse by 0.16 kPa and 0.33 kPa at two different depths. These results demonstrated the feasibility and potentiality of the coded vibration for enhancing the quality of elasticity measurement and imaging.

High-frequency Total Focusing Method (TFM) imaging in strongly attenuating materials with the decomposition of the time reversal operator associated with orthogonal coded excitations

NASA Astrophysics Data System (ADS)

Villaverde, Eduardo Lopez; Robert, Sébastien; Prada, Claire

2017-02-01

In the present work, the Total Focusing Method (TFM) is used to image defects in a High Density Polyethylene (HDPE) pipe. The viscoelastic attenuation of this material corrupts the images with a high electronic noise. In order to improve the image quality, the Decomposition of the Time Reversal Operator (DORT) filtering is combined with spatial Walsh-Hadamard coded transmissions before calculating the images. Experiments on a complex HDPE joint demonstrate that this method improves the signal-to-noise ratio by more than 40 dB in comparison with the conventional TFM.

High-frequency Pulse-compression Ultrasound Imaging with an Annular Array

NASA Astrophysics Data System (ADS)

Mamou, J.; Ketterling, J. A.; Silverman, R. H.

High-frequency ultrasound (HFU) allows fine-resolution imaging at the expense of limited depth-of-field (DOF) and shallow acoustic penetration depth. Coded-excitation imaging permits a significant increase in the signal-to-noise ratio (SNR) and therefore, the acoustic penetration depth. A 17-MHz, five-element annular array with a focal length of 31 mm and a total aperture of 10 mm was fabricated using a 25-μm thick piezopolymer membrane. An optimized 8-μs linear chirp spanning 6.5-32 MHz was used to excite the transducer. After data acquisition, the received signals were linearly filtered by a compression filter and synthetically focused. To compare the chirp-array imaging method with conventional impulse imaging in terms of resolution, a 25-μm wire was scanned and the -6-dB axial and lateral resolutions were computed at depths ranging from 20.5 to 40.5 mm. A tissue-mimicking phantom containing 10-μm glass beads was scanned, and backscattered signals were analyzed to evaluate SNR and penetration depth. Finally, ex-vivo ophthalmic images were formed and chirp-coded images showed features that were not visible in conventional impulse images.

A Huygens Surface Approach to Antenna Implementation in Near-Field Radar Imaging System Simulations

DTIC Science & Technology

2015-08-01

environment. The model consists of an ultra - wideband , forward-looking radar imaging system, equipped with a multi-static antenna array and mounted on a...of the receiving antenna. 2.2 Huygens Surface Implementation Details The NAFDTD code implements the excitation waveform as a short, ultra - wideband ...

Nonlinear pulse compression in pulse-inversion fundamental imaging.

PubMed

Cheng, Yun-Chien; Shen, Che-Chou; Li, Pai-Chi

2007-04-01

Coded excitation can be applied in ultrasound contrast agent imaging to enhance the signal-to-noise ratio with minimal destruction of the microbubbles. Although the axial resolution is usually compromised by the requirement for a long coded transmit waveforms, this can be restored by using a compression filter to compress the received echo. However, nonlinear responses from microbubbles may cause difficulties in pulse compression and result in severe range side-lobe artifacts, particularly in pulse-inversion-based (PI) fundamental imaging. The efficacy of pulse compression in nonlinear contrast imaging was evaluated by investigating several factors relevant to PI fundamental generation using both in-vitro experiments and simulations. The results indicate that the acoustic pressure and the bubble size can alter the nonlinear characteristics of microbubbles and change the performance of the compression filter. When nonlinear responses from contrast agents are enhanced by using a higher acoustic pressure or when more microbubbles are near the resonance size of the transmit frequency, higher range side lobes are produced in both linear imaging and PI fundamental imaging. On the other hand, contrast detection in PI fundamental imaging significantly depends on the magnitude of the nonlinear responses of the bubbles and thus the resultant contrast-to-tissue ratio (CTR) still increases with acoustic pressure and the nonlinear resonance of microbubbles. It should be noted, however, that the CTR in PI fundamental imaging after compression is consistently lower than that before compression due to obvious side-lobe artifacts. Therefore, the use of coded excitation is not beneficial in PI fundamental contrast detection.

Chirped or time modulated excitation compared to short pulses for photoacoustic imaging in acoustic attenuating media

NASA Astrophysics Data System (ADS)

Burgholzer, P.; Motz, C.; Lang, O.; Berer, T.; Huemer, M.

2018-02-01

In photoacoustic imaging, optically generated acoustic waves transport the information about embedded structures to the sample surface. Usually, short laser pulses are used for the acoustic excitation. Acoustic attenuation increases for higher frequencies, which reduces the bandwidth and limits the spatial resolution. One could think of more efficient waveforms than single short pulses, such as pseudo noise codes, chirped, or harmonic excitation, which could enable a higher information-transfer from the samples interior to its surface by acoustic waves. We used a linear state space model to discretize the wave equation, such as the Stoke's equation, but this method could be used for any other linear wave equation. Linear estimators and a non-linear function inversion were applied to the measured surface data, for onedimensional image reconstruction. The proposed estimation method allows optimizing the temporal modulation of the excitation laser such that the accuracy and spatial resolution of the reconstructed image is maximized. We have restricted ourselves to one-dimensional models, as for higher dimensions the one-dimensional reconstruction, which corresponds to the acoustic wave without attenuation, can be used as input for any ultrasound imaging method, such as back-projection or time-reversal method.

Imaging of human tooth using ultrasound based chirp-coded nonlinear time reversal acoustics.

PubMed

Dos Santos, Serge; Prevorovsky, Zdenek

2011-08-01

Human tooth imaging sonography is investigated experimentally with an acousto-optic noncoupling set-up based on the chirp-coded nonlinear time reversal acoustic concept. The complexity of the tooth internal structure (enamel-dentine interface, cracks between internal tubules) is analyzed by adapting the nonlinear elastic wave spectroscopy (NEWS) with the objective of the tomography of damage. Optimization of excitations using intrinsic symmetries, such as time reversal (TR) invariance, reciprocity, correlation properties are then proposed and implemented experimentally. The proposed medical application of this TR-NEWS approach is implemented on a third molar human tooth and constitutes an alternative of noncoupling echodentography techniques. A 10 MHz bandwidth ultrasonic instrumentation has been developed including a laser vibrometer and a 20 MHz contact piezoelectric transducer. The calibrated chirp-coded TR-NEWS imaging of the tooth is obtained using symmetrized excitations, pre- and post-signal processing, and the highly sensitive 14 bit resolution TR-NEWS instrumentation previously calibrated. Nonlinear signature coming from the symmetry properties is observed experimentally in the tooth using this bi-modal TR-NEWS imaging after and before the focusing induced by the time-compression process. The TR-NEWS polar B-scan of the tooth is described and suggested as a potential application for modern echodentography. It constitutes the basis of the self-consistent harmonic imaging sonography for monitoring cracks propagation in the dentine, responsible of human tooth structural health. Copyright © 2011 Elsevier B.V. All rights reserved.

Low sidelobe level and high time resolution for metallic ultrasonic testing with linear-chirp-Golay coded excitation

NASA Astrophysics Data System (ADS)

Zhang, Jiaying; Gang, Tie; Ye, Chaofeng; Cong, Sen

2018-04-01

Linear-chirp-Golay (LCG)-coded excitation combined with pulse compression is proposed in this paper to improve the time resolution and suppress sidelobe in ultrasonic testing. The LCG-coded excitation is binary complementary pair Golay signal with linear-chirp signal applied on every sub pulse. Compared with conventional excitation which is a common ultrasonic testing method using a brief narrow pulse as exciting signal, the performances of LCG-coded excitation, in terms of time resolution improvement and sidelobe suppression, are studied via numerical and experimental investigations. The numerical simulations are implemented using Matlab K-wave toolbox. It is seen from the simulation results that time resolution of LCG excitation is 35.5% higher and peak sidelobe level (PSL) is 57.6 dB lower than linear-chirp excitation with 2.4 MHz chirp bandwidth and 3 μs time duration. In the B-scan experiment, time resolution of LCG excitation is higher and PSL is lower than conventional brief pulse excitation and chirp excitation. In terms of time resolution, LCG-coded signal has better performance than chirp signal. Moreover, the impact of chirp bandwidth on LCG-coded signal is less than that on chirp signal. In addition, the sidelobe of LCG-coded signal is lower than that of chirp signal with pulse compression.

Application of golay complementary coded excitation schemes for non-destructive testing of sandwich structures

NASA Astrophysics Data System (ADS)

Arora, Vanita; Mulaveesala, Ravibabu

2017-06-01

In recent years, InfraRed Thermography (IRT) has become a widely accepted non-destructive testing technique to evaluate the structural integrity of composite sandwich structures due to its full-field, remote, fast and in-service inspection capabilities. This paper presents a novel infrared thermographic approach named as Golay complementary coded thermal wave imaging is presented to detect disbonds in a sandwich structure having face sheets from Glass/Carbon Fibre Reinforced (GFR/CFR) laminates and core of the wooden block.

The Cadmium Zinc Telluride Imager on AstroSat

NASA Astrophysics Data System (ADS)

Bhalerao, V.; Bhattacharya, D.; Vibhute, A.; Pawar, P.; Rao, A. R.; Hingar, M. K.; Khanna, Rakesh; Kutty, A. P. K.; Malkar, J. P.; Patil, M. H.; Arora, Y. K.; Sinha, S.; Priya, P.; Samuel, Essy; Sreekumar, S.; Vinod, P.; Mithun, N. P. S.; Vadawale, S. V.; Vagshette, N.; Navalgund, K. H.; Sarma, K. S.; Pandiyan, R.; Seetha, S.; Subbarao, K.

2017-06-01

The Cadmium Zinc Telluride Imager (CZTI) is a high energy, wide-field imaging instrument on AstroSat. CZTI's namesake Cadmium Zinc Telluride detectors cover an energy range from 20 keV to >200 keV, with 11% energy resolution at 60 keV. The coded aperture mask attains an angular resolution of 17^' over a 4.6° × 4.6° (FWHM) field-of-view. CZTI functions as an open detector above 100 keV, continuously sensitive to GRBs and other transients in about 30% of the sky. The pixellated detectors are sensitive to polarization above ˜ 100 keV, with exciting possibilities for polarization studies of transients and bright persistent sources. In this paper, we provide details of the complete CZTI instrument, detectors, coded aperture mask, mechanical and electronic configuration, as well as data and products.

High-Frequency Chirp Ultrasound Imaging with an Annular-array for Ophthalmologic and Small-Animal Imaging

PubMed Central

Mamou, Jonathan; Aristizábal, Orlando; Silverman, Ronald H.; Ketterling, Jeffrey A.; Turnbull, Daniel H.

2009-01-01

High-frequency ultrasound (HFU, > 20 MHz) is an attractive means of obtaining fine-resolution images of biological tissues for ophthalmologic, dermatological, and small-animal imaging applications. Even with current improvements in circuit designs and high-frequency equipment, HFU suffers from two inherent limitations. First, HFU images have a limited depth of field (DOF) because of the short wavelength and the low fixed F-number of conventional HFU transducers. Second, HFU is usually limited to shallow imaging because of the significant attenuation in most tissues. In a previous study, a five-element annular array with a 17-MHz center frequency was excited using chirp-coded signals, and a synthetic-focusing algorithm was used to extend the DOF and increase penetration depth. In the present study, a similar approach with two different five-element annular arrays operating near a center frequency of 35-MHz is implemented and validated. Following validation studies, the chirp-imaging methods were applied to imaging vitreous-hemorrhage mimicking phantoms and mouse embryos. Images of the vitreous phantom showed increased sensitivity using the chirp method compared to a standard monocycle imaging method, and blood droplets could be visualized 4 mm deeper into the phantom. Three-dimensional datasets of 12.5-day-old, mouse-embryo heads were acquired in utero using chirp and conventional excitations. Images were formed and brains ventricles were segmented and reconstructed in three dimensions. The brain-ventricle volumes for the monocycle excitation exhibited artifacts that were not apparent on the chirp-based dataset reconstruction. PMID:19394754

AAPM/RSNA physics tutorial for residents. Topics in US: B-mode US: basic concepts and new technology.

PubMed

Hangiandreou, Nicholas J

2003-01-01

Ultrasonography (US) has been used in medical imaging for over half a century. Current US scanners are based largely on the same basic principles used in the initial devices for human imaging. Modern equipment uses a pulse-echo approach with a brightness-mode (B-mode) display. Fundamental aspects of the B-mode imaging process include basic ultrasound physics, interactions of ultrasound with tissue, ultrasound pulse formation, scanning the ultrasound beam, and echo detection and signal processing. Recent technical innovations that have been developed to improve the performance of modern US equipment include the following: tissue harmonic imaging, spatial compound imaging, extended field of view imaging, coded pulse excitation, electronic section focusing, three-dimensional and four-dimensional imaging, and the general trend toward equipment miniaturization. US is a relatively inexpensive, portable, safe, and real-time modality, all of which make it one of the most widely used imaging modalities in medicine. Although B-mode US is sometimes referred to as a mature technology, this modality continues to experience a significant evolution in capability with even more exciting developments on the horizon. Copyright RSNA, 2003

Space-time encoding for high frame rate ultrasound imaging.

PubMed

Misaridis, Thanassis X; Jensen, Jørgen A

2002-05-01

Frame rate in ultrasound imaging can be dramatically increased by using sparse synthetic transmit aperture (STA) beamforming techniques. The two main drawbacks of the method are the low signal-to-noise ratio (SNR) and the motion artifacts, that degrade the image quality. In this paper we propose a spatio-temporal encoding for STA imaging based on simultaneous transmission of two quasi-orthogonal tapered linear FM signals. The excitation signals are an up- and a down-chirp with frequency division and a cross-talk of -55 dB. The received signals are first cross-correlated with the appropriate code, then spatially decoded and finally beamformed for each code, yielding two images per emission. The spatial encoding is a Hadamard encoding previously suggested by Chiao et al. [in: Proceedings of the IEEE Ultrasonics Symposium, 1997, p. 1679]. The Hadamard matrix has half the size of the transmit element groups, due to the orthogonality of the temporal encoded wavefronts. Thus, with this method, the frame rate is doubled compared to previous systems. Another advantage is the utilization of temporal codes which are more robust to attenuation. With the proposed technique it is possible to obtain images dynamically focused in both transmit and receive with only two firings. This reduces the problem of motion artifacts. The method has been tested with extensive simulations using Field II. Resolution and SNR are compared with uncoded STA imaging and conventional phased-array imaging. The range resolution remains the same for coded STA imaging with four emissions and is slightly degraded for STA imaging with two emissions due to the -55 dB cross-talk between the signals. The additional proposed temporal encoding adds more than 15 dB on the SNR gain, yielding a SNR at the same order as in phased-array imaging.

Large-scale transmission-type multifunctional anisotropic coding metasurfaces in millimeter-wave frequencies

NASA Astrophysics Data System (ADS)

Cui, Tie Jun; Wu, Rui Yuan; Wu, Wei; Shi, Chuan Bo; Li, Yun Bo

2017-10-01

We propose fast and accurate designs to large-scale and low-profile transmission-type anisotropic coding metasurfaces with multiple functions in the millimeter-wave frequencies based on the antenna-array method. The numerical simulation of an anisotropic coding metasurface with the size of 30λ × 30λ by the proposed method takes only 20 min, which however cannot be realized by commercial software due to huge memory usage in personal computers. To inspect the performance of coding metasurfaces in the millimeter-wave band, the working frequency is chosen as 60 GHz. Based on the convolution operations and holographic theory, the proposed multifunctional anisotropic coding metasurface exhibits different effects excited by y-polarized and x-polarized incidences. This study extends the frequency range of coding metasurfaces, filling the gap between microwave and terahertz bands, and implying promising applications in millimeter-wave communication and imaging.

Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules.

PubMed

Han, M; Gao, X; Su, J Z; Nie, S

2001-07-01

Multicolor optical coding for biological assays has been achieved by embedding different-sized quantum dots (zinc sulfide-capped cadmium selenide nanocrystals) into polymeric microbeads at precisely controlled ratios. Their novel optical properties (e.g., size-tunable emission and simultaneous excitation) render these highly luminescent quantum dots (QDs) ideal fluorophores for wavelength-and-intensity multiplexing. The use of 10 intensity levels and 6 colors could theoretically code one million nucleic acid or protein sequences. Imaging and spectroscopic measurements indicate that the QD-tagged beads are highly uniform and reproducible, yielding bead identification accuracies as high as 99.99% under favorable conditions. DNA hybridization studies demonstrate that the coding and target signals can be simultaneously read at the single-bead level. This spectral coding technology is expected to open new opportunities in gene expression studies, high-throughput screening, and medical diagnostics.

An ultrasound transient elastography system with coded excitation.

PubMed

Diao, Xianfen; Zhu, Jing; He, Xiaonian; Chen, Xin; Zhang, Xinyu; Chen, Siping; Liu, Weixiang

2017-06-28

Ultrasound transient elastography technology has found its place in elastography because it is safe and easy to operate. However, it's application in deep tissue is limited. The aim of this study is to design an ultrasound transient elastography system with coded excitation to obtain greater detection depth. The ultrasound transient elastography system requires tissue vibration to be strictly synchronous with ultrasound detection. Therefore, an ultrasound transient elastography system with coded excitation was designed. A central component of this transient elastography system was an arbitrary waveform generator with multi-channel signals output function. This arbitrary waveform generator was used to produce the tissue vibration signal, the ultrasound detection signal and the synchronous triggering signal of the radio frequency data acquisition system. The arbitrary waveform generator can produce different forms of vibration waveform to induce different shear wave propagation in the tissue. Moreover, it can achieve either traditional pulse-echo detection or a phase-modulated or a frequency-modulated coded excitation. A 7-chip Barker code and traditional pulse-echo detection were programmed on the designed ultrasound transient elastography system to detect the shear wave in the phantom excited by the mechanical vibrator. Then an elasticity QA phantom and sixteen in vitro rat livers were used for performance evaluation of the two detection pulses. The elasticity QA phantom's results show that our system is effective, and the rat liver results show the detection depth can be increased more than 1 cm. In addition, the SNR (signal-to-noise ratio) is increased by 15 dB using the 7-chip Barker coded excitation. Applying 7-chip Barker coded excitation technique to the ultrasound transient elastography can increase the detection depth and SNR. Using coded excitation technology to assess the human liver, especially in obese patients, may be a good choice.

MOM3D method of moments code theory manual

NASA Technical Reports Server (NTRS)

Shaeffer, John F.

1992-01-01

MOM3D is a FORTRAN algorithm that solves Maxwell's equations as expressed via the electric field integral equation for the electromagnetic response of open or closed three dimensional surfaces modeled with triangle patches. Two joined triangles (couples) form the vector current unknowns for the surface. Boundary conditions are for perfectly conducting or resistive surfaces. The impedance matrix represents the fundamental electromagnetic interaction of the body with itself. A variety of electromagnetic analysis options are possible once the impedance matrix is computed including backscatter radar cross section (RCS), bistatic RCS, antenna pattern prediction for user specified body voltage excitation ports, RCS image projection showing RCS scattering center locations, surface currents excited on the body as induced by specified plane wave excitation, and near field computation for the electric field on or near the body.

Ion Kinetics in Silane Plasmas

DTIC Science & Technology

1988-04-20

field and orthogonal to the excite plates. The image current is amplified, digitized, and Fourier analyzed to yield a spectrum of 0 cyclotron...Laboratory (AFWAL/P0OC). 17. COSATI CODES 18. SUBJECT TERMS (Continue on reverse if necessary and identify by block number) FIELD GROUP SUB-GROUP 20 U9 09 03...d.c., microwave, or capacatively coupled, radiofrequency electric fields . Alternatively, hollow cath- ode or electron beam approaches may be employed

Signal Prediction With Input Identification

NASA Technical Reports Server (NTRS)

Juang, Jer-Nan; Chen, Ya-Chin

1999-01-01

A novel coding technique is presented for signal prediction with applications including speech coding, system identification, and estimation of input excitation. The approach is based on the blind equalization method for speech signal processing in conjunction with the geometric subspace projection theory to formulate the basic prediction equation. The speech-coding problem is often divided into two parts, a linear prediction model and excitation input. The parameter coefficients of the linear predictor and the input excitation are solved simultaneously and recursively by a conventional recursive least-squares algorithm. The excitation input is computed by coding all possible outcomes into a binary codebook. The coefficients of the linear predictor and excitation, and the index of the codebook can then be used to represent the signal. In addition, a variable-frame concept is proposed to block the same excitation signal in sequence in order to reduce the storage size and increase the transmission rate. The results of this work can be easily extended to the problem of disturbance identification. The basic principles are outlined in this report and differences from other existing methods are discussed. Simulations are included to demonstrate the proposed method.

Pulse Vector-Excitation Speech Encoder

NASA Technical Reports Server (NTRS)

Davidson, Grant; Gersho, Allen

1989-01-01

Proposed pulse vector-excitation speech encoder (PVXC) encodes analog speech signals into digital representation for transmission or storage at rates below 5 kilobits per second. Produces high quality of reconstructed speech, but with less computation than required by comparable speech-encoding systems. Has some characteristics of multipulse linear predictive coding (MPLPC) and of code-excited linear prediction (CELP). System uses mathematical model of vocal tract in conjunction with set of excitation vectors and perceptually-based error criterion to synthesize natural-sounding speech.

Speech coding at low to medium bit rates

NASA Astrophysics Data System (ADS)

Leblanc, Wilfred Paul

1992-09-01

Improved search techniques coupled with improved codebook design methodologies are proposed to improve the performance of conventional code-excited linear predictive coders for speech. Improved methods for quantizing the short term filter are developed by employing a tree search algorithm and joint codebook design to multistage vector quantization. Joint codebook design procedures are developed to design locally optimal multistage codebooks. Weighting during centroid computation is introduced to improve the outlier performance of the multistage vector quantizer. Multistage vector quantization is shown to be both robust against input characteristics and in the presence of channel errors. Spectral distortions of about 1 dB are obtained at rates of 22-28 bits/frame. Structured codebook design procedures for excitation in code-excited linear predictive coders are compared to general codebook design procedures. Little is lost using significant structure in the excitation codebooks while greatly reducing the search complexity. Sparse multistage configurations are proposed for reducing computational complexity and memory size. Improved search procedures are applied to code-excited linear prediction which attempt joint optimization of the short term filter, the adaptive codebook, and the excitation. Improvements in signal to noise ratio of 1-2 dB are realized in practice.

Vector Adaptive/Predictive Encoding Of Speech

NASA Technical Reports Server (NTRS)

Chen, Juin-Hwey; Gersho, Allen

1989-01-01

Vector adaptive/predictive technique for digital encoding of speech signals yields decoded speech of very good quality after transmission at coding rate of 9.6 kb/s and of reasonably good quality at 4.8 kb/s. Requires 3 to 4 million multiplications and additions per second. Combines advantages of adaptive/predictive coding, and code-excited linear prediction, yielding speech of high quality but requires 600 million multiplications and additions per second at encoding rate of 4.8 kb/s. Vector adaptive/predictive coding technique bridges gaps in performance and complexity between adaptive/predictive coding and code-excited linear prediction.

Signal intensity analysis and optimization for in vivo imaging of Cherenkov and excited luminescence

NASA Astrophysics Data System (ADS)

LaRochelle, Ethan P. M.; Shell, Jennifer R.; Gunn, Jason R.; Davis, Scott C.; Pogue, Brian W.

2018-04-01

During external beam radiotherapy (EBRT), in vivo Cherenkov optical emissions can be used as a dosimetry tool or to excite luminescence, termed Cherenkov-excited luminescence (CEL) with microsecond-level time-gated cameras. The goal of this work was to develop a complete theoretical foundation for the detectable signal strength, in order to provide guidance on optimization of the limits of detection and how to optimize near real time imaging. The key parameters affecting photon production, propagation and detection were considered and experimental validation with both tissue phantoms and a murine model are shown. Both the theoretical analysis and experimental data indicate that the detection level is near a single photon-per-pixel for the detection geometry and frame rates commonly used, with the strongest factor being the signal decrease with the square of distance from tissue to camera. Experimental data demonstrates how the SNR improves with increasing integration time, but only up to the point where the dominance of camera read noise is overcome by stray photon noise that cannot be suppressed. For the current camera in a fixed geometry, the signal to background ratio limits the detection of light signals, and the observed in vivo Cherenkov emission is on the order of 100×  stronger than CEL signals. As a result, imaging signals from depths  <15 mm is reasonable for Cherenkov light, and depths  <3 mm is reasonable for CEL imaging. The current investigation modeled Cherenkov and CEL imaging of two oxygen sensing phosphorescent compounds, but the modularity of the code allows for easy comparison of different agents or alternative cameras, geometries or tissues.

Signal intensity analysis and optimization for in vivo imaging of Cherenkov and excited luminescence.

PubMed

LaRochelle, Ethan P M; Shell, Jennifer R; Gunn, Jason R; Davis, Scott C; Pogue, Brian W

2018-04-20

During external beam radiotherapy (EBRT), in vivo Cherenkov optical emissions can be used as a dosimetry tool or to excite luminescence, termed Cherenkov-excited luminescence (CEL) with microsecond-level time-gated cameras. The goal of this work was to develop a complete theoretical foundation for the detectable signal strength, in order to provide guidance on optimization of the limits of detection and how to optimize near real time imaging. The key parameters affecting photon production, propagation and detection were considered and experimental validation with both tissue phantoms and a murine model are shown. Both the theoretical analysis and experimental data indicate that the detection level is near a single photon-per-pixel for the detection geometry and frame rates commonly used, with the strongest factor being the signal decrease with the square of distance from tissue to camera. Experimental data demonstrates how the SNR improves with increasing integration time, but only up to the point where the dominance of camera read noise is overcome by stray photon noise that cannot be suppressed. For the current camera in a fixed geometry, the signal to background ratio limits the detection of light signals, and the observed in vivo Cherenkov emission is on the order of 100×  stronger than CEL signals. As a result, imaging signals from depths  <15 mm is reasonable for Cherenkov light, and depths  <3 mm is reasonable for CEL imaging. The current investigation modeled Cherenkov and CEL imaging of two oxygen sensing phosphorescent compounds, but the modularity of the code allows for easy comparison of different agents or alternative cameras, geometries or tissues.

Topological entanglement entropy with a twist.

PubMed

Brown, Benjamin J; Bartlett, Stephen D; Doherty, Andrew C; Barrett, Sean D

2013-11-27

Defects in topologically ordered models have interesting properties that are reminiscent of the anyonic excitations of the models themselves. For example, dislocations in the toric code model are known as twists and possess properties that are analogous to Ising anyons. We strengthen this analogy by using the topological entanglement entropy as a diagnostic tool to identify properties of both defects and excitations in the toric code. Specifically, we show, through explicit calculation, that the toric code model including twists and dyon excitations has the same quantum dimensions, the same total quantum dimension, and the same fusion rules as an Ising anyon model.

Treatment of isomers in nucleosynthesis codes

NASA Astrophysics Data System (ADS)

Reifarth, René; Fiebiger, Stefan; Göbel, Kathrin; Heftrich, Tanja; Kausch, Tanja; Köppchen, Christoph; Kurtulgil, Deniz; Langer, Christoph; Thomas, Benedikt; Weigand, Mario

2018-03-01

The decay properties of long-lived excited states (isomers) can have a significant impact on the destruction channels of isotopes under stellar conditions. In sufficiently hot environments, the population of isomers can be altered via thermal excitation or de-excitation. If the corresponding lifetimes are of the same order of magnitude as the typical time scales of the environment, the isomers have to be treated explicitly. We present a general approach to the treatment of isomers in stellar nucleosynthesis codes and discuss a few illustrative examples. The corresponding code is available online at http://exp-astro.de/isomers/.

Technology Needs for Gamma Ray Astronomy

NASA Technical Reports Server (NTRS)

Gehrels, Neil

2011-01-01

Gamma ray astronomy is currently in an exciting period of multiple missions and a wealth of data. Results from INTEGRAL, Fermi, AGILE, Suzaku and Swift are making large contributions to our knowledge of high energy processes in the universe. The advances are due to new detector and imaging technologies. The steps to date have been from scintillators to solid state detectors for sensors and from light buckets to coded aperture masks and pair telescopes for imagers. A key direction for the future is toward focusing telescopes pushing into the hard X-ray regime and Compton telescopes and pair telescopes with fine spatial resolution for medium and high energy gamma rays. These technologies will provide finer imaging of gamma-ray sources. Importantly, they will also enable large steps forward in sensitivity by reducing background.

Computational simulation of acoustic fatigue for hot composite structures

NASA Technical Reports Server (NTRS)

Singhal, S. N.; Nagpal, V. K.; Murthy, P. L. N.; Chamis, C. C.

1991-01-01

This paper presents predictive methods/codes for computational simulation of acoustic fatigue resistance of hot composite structures subjected to acoustic excitation emanating from an adjacent vibrating component. Select codes developed over the past two decades at the NASA Lewis Research Center are used. The codes include computation of (1) acoustic noise generated from a vibrating component, (2) degradation in material properties of the composite laminate at use temperature, (3) dynamic response of acoustically excited hot multilayered composite structure, (4) degradation in the first-ply strength of the excited structure due to acoustic loading, and (5) acoustic fatigue resistance of the excited structure, including propulsion environment. Effects of the laminate lay-up and environment on the acoustic fatigue life are evaluated. The results show that, by keeping the angled plies on the outer surface of the laminate, a substantial increase in the acoustic fatigue life is obtained. The effect of environment (temperature and moisure) is to relieve the residual stresses leading to an increase in the acoustic fatigue life of the excited panel.

Computational simulation of acoustic fatigue for hot composite structures

NASA Technical Reports Server (NTRS)

Singhal, Surendra N.; Murthy, Pappu L. N.; Chamis, Christos C.; Nagpal, Vinod K.; Sutjahjo, Edhi

1991-01-01

Predictive methods/computer codes for the computational simulation of acoustic fatigue resistance of hot composite structures subjected to acoustic excitation emanating from an adjacent vibrating component are discussed. Select codes developed over the past two decades at the NASA Lewis Research Center are used. The codes include computation of acoustic noise generated from a vibrating component, degradation in material properties of a composite laminate at use temperature, dynamic response of acoustically excited hot multilayered composite structure, degradation in the first ply strength of the excited structure due to acoustic loading, and acoustic fatigue resistance of the excited structure, including the propulsion environment. Effects of the laminate lay-up and environment on the acoustic fatigue life are evaluated. The results show that, by keeping the angled plies on the outer surface of the laminate, a substantial increase in the acoustic fatigue life is obtained. The effect of environment (temperature and moisture) is to relieve the residual stresses leading to an increase in the acoustic fatigue life of the excited panel.

Photoionization and High Density Gas

NASA Technical Reports Server (NTRS)

Kallman, T.; Bautista, M.; White, Nicholas E. (Technical Monitor)

2002-01-01

We present results of calculations using the XSTAR version 2 computer code. This code is loosely based on the XSTAR v.1 code which has been available for public use for some time. However it represents an improvement and update in several major respects, including atomic data, code structure, user interface, and improved physical description of ionization/excitation. In particular, it now is applicable to high density situations in which significant excited atomic level populations are likely to occur. We describe the computational techniques and assumptions, and present sample runs with particular emphasis on high density situations.

Coded excitation for infrared non-destructive testing of carbon fiber reinforced plastics.

PubMed

Mulaveesala, Ravibabu; Venkata Ghali, Subbarao

2011-05-01

This paper proposes a Barker coded excitation for defect detection using infrared non-destructive testing. Capability of the proposed excitation scheme is highlighted with recently introduced correlation based post processing approach and compared with the existing phase based analysis by taking the signal to noise ratio into consideration. Applicability of the proposed scheme has been experimentally validated on a carbon fiber reinforced plastic specimen containing flat bottom holes located at different depths.

Echodentography based on nonlinear time reversal tomography: Ultrasonic nonlinear signature identification

NASA Astrophysics Data System (ADS)

Santos, Serge Dos; Farova, Zuzana; Kus, Vaclav; Prevorovsky, Zdenek

2012-05-01

This paper examines possibilities of using Nonlinear Elastic Wave Spectroscopy (NEWS) methods in dental investigations. Themain task consisted in imaging cracks or other degradation signatures located in dentin close to the Enamel-Dentine Junction (EDJ). NEWS approach was investigated experimentally with a new bi-modal acousto-optic set-up based on the chirp-coded nonlinear ultrasonic time reversal (TR) concepts. Complex internal structure of the tooth is analyzed by the TR-NEWS procedure adapted to tomography-like imaging of the tooth damages. Ultrasonic instrumentation with 10 MHz bandwidth has been set together including laser vibrometer used to detect responses of the tooth on its excitation carried out by a contact piezoelectric transducer. Bi-modal TR-NEWS images of the tooth were created before and after focusing, which resulted from the time compression. The polar B-scan of the tooth realized with TR-NEWS procedure is suggested to be applied as a new echodentography imaging.

Local spatio-temporal analysis in vision systems

NASA Astrophysics Data System (ADS)

Geisler, Wilson S.; Bovik, Alan; Cormack, Lawrence; Ghosh, Joydeep; Gildeen, David

1994-07-01

The aims of this project are the following: (1) develop a physiologically and psychophysically based model of low-level human visual processing (a key component of which are local frequency coding mechanisms); (2) develop image models and image-processing methods based upon local frequency coding; (3) develop algorithms for performing certain complex visual tasks based upon local frequency representations, (4) develop models of human performance in certain complex tasks based upon our understanding of low-level processing; and (5) develop a computational testbed for implementing, evaluating and visualizing the proposed models and algorithms, using a massively parallel computer. Progress has been substantial on all aims. The highlights include the following: (1) completion of a number of psychophysical and physiological experiments revealing new, systematic and exciting properties of the primate (human and monkey) visual system; (2) further development of image models that can accurately represent the local frequency structure in complex images; (3) near completion in the construction of the Texas Active Vision Testbed; (4) development and testing of several new computer vision algorithms dealing with shape-from-texture, shape-from-stereo, and depth-from-focus; (5) implementation and evaluation of several new models of human visual performance; and (6) evaluation, purchase and installation of a MasPar parallel computer.

Three-photon tissue imaging using moxifloxacin.

PubMed

Lee, Seunghun; Lee, Jun Ho; Wang, Taejun; Jang, Won Hyuk; Yoon, Yeoreum; Kim, Bumju; Jun, Yong Woong; Kim, Myoung Joon; Kim, Ki Hean

2018-06-20

Moxifloxacin is an antibiotic used in clinics and has recently been used as a clinically compatible cell-labeling agent for two-photon (2P) imaging. Although 2P imaging with moxifloxacin labeling visualized cells inside tissues using enhanced fluorescence, the imaging depth was quite limited because of the relatively short excitation wavelength (<800 nm) used. In this study, the feasibility of three-photon (3P) excitation of moxifloxacin using a longer excitation wavelength and moxifloxacin-based 3P imaging were tested to increase the imaging depth. Moxifloxacin fluorescence via 3P excitation was detected at a >1000 nm excitation wavelength. After obtaining the excitation and emission spectra of moxifloxacin, moxifloxacin-based 3P imaging was applied to ex vivo mouse bladder and ex vivo mouse small intestine tissues and compared with moxifloxacin-based 2P imaging by switching the excitation wavelength of a Ti:sapphire oscillator between near 1030 and 780 nm. Both moxifloxacin-based 2P and 3P imaging visualized cellular structures in the tissues via moxifloxacin labeling, but the image contrast was better with 3P imaging than with 2P imaging at the same imaging depths. The imaging speed and imaging depth of moxifloxacin-based 3P imaging using a Ti:sapphire oscillator were limited by insufficient excitation power. Therefore, we constructed a new system for moxifloxacin-based 3P imaging using a high-energy Yb fiber laser at 1030 nm and used it for in vivo deep tissue imaging of a mouse small intestine. Moxifloxacin-based 3P imaging could be useful for clinical applications with enhanced imaging depth.

Physical limits to autofluorescence signals in vivo recordings in the rat olfactory bulb: a Monte Carlo study

NASA Astrophysics Data System (ADS)

L'Heureux, B.; Gurden, H.; Pinot, L.; Mastrippolito, R.; Lefebvre, F.; Lanièce, P.; Pain, F.

2007-07-01

Understanding the cellular mechanisms of energy supply to neurons following physiological activation is still challenging and has strong implications to the interpretation of clinical functional images based on metabolic signals such as Blood Oxygen Level Dependent Magnetic Resonance Imaging or 18F-Fluorodexoy-Glucose Positron Emission Tomography. Intrinsic Optical Signal Imaging provides with high spatio temporal resolution in vivo imaging in the anaesthetized rat. In that context, intrinsic signals are mainly related to changes in the optical absorption of haemoglobin depending on its oxygenation state. This technique has been validated for imaging of the rat olfactory bulb, providing with maps of the actived olfactory glomeruli, the functional modules involved in the first step of olfactory coding. A complementary approach would be autofluorescence imaging relying on the fluorescence properties of endogenous Flavin Adenine Dinucleotide (FAD) or Nicotinamide Adenine Dinucleotide (NADH) both involved in intracellular metabolic pathways. The purpose of the present study was to investigate the feasibility of in vivo autofluorescence imaging in the rat olfactory bulb. We performed standard Monte Carlo simulations of photons scattering and absorption at the excitation and emission wavelengths of FAD and NADH fluorescence. Characterization of the fluorescence distribution in the glomerulus, effect of hemoglobin absorption at the excitation and absorption wavelengths as well as the effect of the blurring due to photon scattering and the depth of focus of the optical apparatus have been studied. Finally, optimal experimental parameters are proposed to achieve in vivo validation of the technique in the rat olfactory bulb.

Global modeling of thermospheric airglow in the far ultraviolet

NASA Astrophysics Data System (ADS)

Solomon, Stanley C.

2017-07-01

The Global Airglow (GLOW) model has been updated and extended to calculate thermospheric emissions in the far ultraviolet, including sources from daytime photoelectron-driven processes, nighttime recombination radiation, and auroral excitation. It can be run using inputs from empirical models of the neutral atmosphere and ionosphere or from numerical general circulation models of the coupled ionosphere-thermosphere system. It uses a solar flux module, photoelectron generation routine, and the Nagy-Banks two-stream electron transport algorithm to simultaneously handle energetic electron distributions from photon and auroral electron sources. It contains an ion-neutral chemistry module that calculates excited and ionized species densities and the resulting airglow volume emission rates. This paper describes the inputs, algorithms, and code structure of the model and demonstrates example outputs for daytime and auroral cases. Simulations of far ultraviolet emissions by the atomic oxygen doublet at 135.6 nm and the molecular nitrogen Lyman-Birge-Hopfield bands, as viewed from geostationary orbit, are shown, and model calculations are compared to limb-scan observations by the Global Ultraviolet Imager on the TIMED satellite. The GLOW model code is provided to the community through an open-source academic research license.

(86)Y production via (86)Sr(p,n) for PET imaging at a cyclotron.

PubMed

Sadeghi, M; Aboudzadeh, M; Zali, A; Zeinali, B

2009-01-01

Excitation functions of (86)Y production via (86)Sr(p,xn), (86)Sr(d,xn), (85)Rb(alpha,xn), (85)Rb((3)He,xn), and (nat)Zr(d,alphaxn) reactions were studied by means of ALICE-ASH code and the results were compared with ALICE-91 code and experimental data. The greatest nuclear reaction of cyclotron (86)Y production was found out as (86)Sr(p,n)(86)Y process. (86)Y production yield was calculated too. A SrCO(3) thick film was deposited on a copper substrate by sedimentation method. The deposited (nat)SrCO(3) was irradiated with 15MeV proton at 30microA current beam. The separation of Y from Cu and Sr was carried out by means of dual ion exchange chromatography.

A Comprehensive C++ Controller for a Magnetically Supported Vertical Rotor. 1.0

NASA Technical Reports Server (NTRS)

Morrison, Carlos R.

2001-01-01

This manual describes the new FATMaCC (Five-Axis, Three-Magnetic-Bearing Control Code). The FATMaCC (pronounced "fat mak") is a versatile control code that possesses many desirable features that were not available in previous in-house controllers. The ultimate goal in designing this code was to achieve full rotor levitation and control at a loop time of 50 microsec. Using a 1-GHz processor, the code will control a five-axis system in either a decentralized or a more elegant centralized (modal control) mode at a loop time of 56 microsec. In addition, it will levitate and control (with only minor modification to the input/output wiring) a two-axis and/or a four-axis system. Stable rotor levitation and control of any of the systems mentioned above are accomplished through appropriate key presses to modify parameters, such as stiffness, damping, and bias. A signal generation block provides 11 excitation signals. An excitation signal is then superimposed on the radial bearing x- and y-control signals, thus producing a resultant force vector. By modulating the signals on the bearing x- and y-axes with a cosine and a sine function, respectively, a radial excitation force vector is made to rotate 360 deg. about the bearing geometric center. The rotation of the force vector is achieved manually by using key press or automatically by engaging the "one-per-revolution" feature. Rotor rigid body modes can be excited by using the excitation module. Depending on the polarities of the excitation signal in each radial bearing, the bounce or tilt mode will be excited.

Non-adiabatic Excited State Molecule Dynamics Modeling of Photochemistry and Photophysics of Materials

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nelson, Tammie Renee; Tretiak, Sergei

2017-01-06

Understanding and controlling excited state dynamics lies at the heart of all our efforts to design photoactive materials with desired functionality. This tailor-design approach has become the standard for many technological applications (e.g., solar energy harvesting) including the design of organic conjugated electronic materials with applications in photovoltaic and light-emitting devices. Over the years, our team has developed efficient LANL-based codes to model the relevant photophysical processes following photoexcitation (spatial energy transfer, excitation localization/delocalization, and/or charge separation). The developed approach allows the non-radiative relaxation to be followed on up to ~10 ps timescales for large realistic molecules (hundreds of atomsmore » in size) in the realistic solvent dielectric environment. The Collective Electronic Oscillator (CEO) code is used to compute electronic excited states, and the Non-adiabatic Excited State Molecular Dynamics (NA-ESMD) code is used to follow the non-adiabatic dynamics on multiple coupled Born-Oppenheimer potential energy surfaces. Our preliminary NA-ESMD simulations have revealed key photoinduced mechanisms controlling competing interactions and relaxation pathways in complex materials, including organic conjugated polymer materials, and have provided a detailed understanding of photochemical products and intermediates and the internal conversion process during the initiation of energetic materials. This project will be using LANL-based CEO and NA-ESMD codes to model nonradiative relaxation in organic and energetic materials. The NA-ESMD and CEO codes belong to a class of electronic structure/quantum chemistry codes that require large memory, “long-queue-few-core” distribution of resources in order to make useful progress. The NA-ESMD simulations are trivially parallelizable requiring ~300 processors for up to one week runtime to reach a meaningful restart point.« less

Hyperspectral imaging fluorescence excitation scanning for colon cancer detection

NASA Astrophysics Data System (ADS)

Leavesley, Silas J.; Walters, Mikayla; Lopez, Carmen; Baker, Thomas; Favreau, Peter F.; Rich, Thomas C.; Rider, Paul F.; Boudreaux, Carole W.

2016-10-01

Optical spectroscopy and hyperspectral imaging have shown the potential to discriminate between cancerous and noncancerous tissue with high sensitivity and specificity. However, to date, these techniques have not been effectively translated to real-time endoscope platforms. Hyperspectral imaging of the fluorescence excitation spectrum represents new technology that may be well suited for endoscopic implementation. However, the feasibility of detecting differences between normal and cancerous mucosa using fluorescence excitation-scanning hyperspectral imaging has not been evaluated. The goal of this study was to evaluate the initial feasibility of using fluorescence excitation-scanning hyperspectral imaging for measuring changes in fluorescence excitation spectrum concurrent with colonic adenocarcinoma using a small pre-pilot-scale sample size. Ex vivo analysis was performed using resected pairs of colorectal adenocarcinoma and normal mucosa. Adenocarcinoma was confirmed by histologic evaluation of hematoxylin and eosin (H&E) permanent sections. Specimens were imaged using a custom hyperspectral imaging fluorescence excitation-scanning microscope system. Results demonstrated consistent spectral differences between normal and cancerous tissues over the fluorescence excitation range of 390 to 450 nm that could be the basis for wavelength-dependent detection of colorectal cancers. Hence, excitation-scanning hyperspectral imaging may offer an alternative approach for discriminating adenocarcinoma from surrounding normal colonic mucosa, but further studies will be required to evaluate the accuracy of this approach using a larger patient cohort.

Neural imaging in songbirds using fiber optic fluorescence microscopy

NASA Astrophysics Data System (ADS)

Nooshabadi, Fatemeh; Hearn, Gentry; Lints, Thierry; Maitland, Kristen C.

2012-02-01

The song control system of juvenile songbirds is an important model for studying the developmental acquisition and generation of complex learned vocal motor sequences, two processes that are fundamental to human speech and language. To understand the neural mechanisms underlying song production, it is critical to characterize the activity of identified neurons in the song control system when the bird is singing. Neural imaging in unrestrained singing birds, although technically challenging, will advance our understanding of neural ensemble coding mechanisms in this system. We are exploring the use of a fiber optic microscope for functional imaging in the brain of behaving and singing birds in order to better understand the contribution of a key brain nucleus (high vocal center nucleus; HVC) to temporal aspects of song motor control. We have constructed a fluorescence microscope with LED illumination, a fiber bundle for transmission of fluorescence excitation and emission light, a ~2x GRIN lens, and a CCD for image acquisition. The system has 2 μm resolution, 375 μm field of view, 200 μm working distance, and 1 mm outer diameter. As an initial characterization of this setup, neurons in HVC were imaged using the fiber optic microscope after injection of quantum dots or fluorescent retrograde tracers into different song nuclei. A Lucid Vivascope confocal microscope was used to confirm the imaging results. Long-term imaging of the activity of these neurons in juvenile birds during singing may lead us to a better understanding of the central motor codes for song and the central mechanism by which auditory experience modifies song motor commands to enable vocal learning and imitation.

Large-Scale Fluorescence Calcium-Imaging Methods for Studies of Long-Term Memory in Behaving Mammals

PubMed Central

Jercog, Pablo; Rogerson, Thomas; Schnitzer, Mark J.

2016-01-01

During long-term memory formation, cellular and molecular processes reshape how individual neurons respond to specific patterns of synaptic input. It remains poorly understood how such changes impact information processing across networks of mammalian neurons. To observe how networks encode, store, and retrieve information, neuroscientists must track the dynamics of large ensembles of individual cells in behaving animals, over timescales commensurate with long-term memory. Fluorescence Ca2+-imaging techniques can monitor hundreds of neurons in behaving mice, opening exciting avenues for studies of learning and memory at the network level. Genetically encoded Ca2+ indicators allow neurons to be targeted by genetic type or connectivity. Chronic animal preparations permit repeated imaging of neural Ca2+ dynamics over multiple weeks. Together, these capabilities should enable unprecedented analyses of how ensemble neural codes evolve throughout memory processing and provide new insights into how memories are organized in the brain. PMID:27048190

Clinical multiphoton and CARS microscopy

NASA Astrophysics Data System (ADS)

Breunig, H. G.; Weinigel, M.; Darvin, M. E.; Lademann, J.; König, K.

2012-03-01

We report on clinical CARS imaging of human skin in vivo with the certified hybrid multiphoton tomograph CARSDermaInspect. The CARS-DermaInspect provides simultaneous imaging of non-fluorescent intradermal lipid and water as well as imaging of two-photon excited fluorescence from intrinsic molecules. Two different excitation schemes for CARS imaging have been realized: In the first setup, a combination of fs oscillator and optical parametric oscillator provided fs-CARS pump and Stokes pulses, respectively. In the second setup a fs oscillator was combined with a photonic crystal fiber which provided a broadband spectrum. A spectral range out of the broadband-spectrum was selected and used for CARS excitation in combination with the residual fs-oscillator output. In both setups, in addition to CARS, single-beam excitation was used for imaging of two-photon excited fluorescence and second harmonic generation signals. Both CARS-excitation systems were successfully used for imaging of lipids inside the skin in vivo.

A NEW RAYTRACER FOR MODELING AU-SCALE IMAGING OF LINES FROM PROTOPLANETARY DISKS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pontoppidan, Klaus M.; Meijerink, Rowin; Blake, Geoffrey A.

The material that formed the present-day solar system originated in feeding zones in the inner solar nebula located at distances within approx20 AU from the Sun, known as the planet-forming zone. Meteoritic and cometary material contain abundant evidence for the presence of a rich and active chemistry in the planet-forming zone during the gas-rich phase of solar system formation. It is a natural conjecture that analogs can be found among the zoo of protoplanetary disks around nearby young stars. The study of the chemistry and dynamics of planet formation requires: (1) tracers of dense gas at 100-1000 K and (2)more » imaging capabilities of such tracers with 5-100 mas (0.5-20 AU) resolution, corresponding to the planet-forming zone at the distance of the closest star-forming regions. Recognizing that the rich infrared (2-200 mum) molecular spectrum recently discovered to be common in protoplanetary disks represents such a tracer, we present a new general ray-tracing code, RADLite, that is optimized for producing infrared line spectra and images from axisymmetric structures. RADLite can consistently deal with a wide range of velocity gradients, such as those typical for the inner regions of protoplanetary disks. The code is intended as a back-end for chemical and excitation codes, and can rapidly produce spectra of thousands of lines for grids of models for comparison with observations. Such radiative transfer tools will be crucial for constraining both the structure and chemistry of planet-forming regions, including data from current infrared imaging spectrometers and extending to the Atacama Large Millimeter Array and the next generation of Extremely Large Telescopes, the James Webb Space Telescope and beyond.« less

Convolutional Sparse Coding for RGB+NIR Imaging.

PubMed

Hu, Xuemei; Heide, Felix; Dai, Qionghai; Wetzstein, Gordon

2018-04-01

Emerging sensor designs increasingly rely on novel color filter arrays (CFAs) to sample the incident spectrum in unconventional ways. In particular, capturing a near-infrared (NIR) channel along with conventional RGB color is an exciting new imaging modality. RGB+NIR sensing has broad applications in computational photography, such as low-light denoising, it has applications in computer vision, such as facial recognition and tracking, and it paves the way toward low-cost single-sensor RGB and depth imaging using structured illumination. However, cost-effective commercial CFAs suffer from severe spectral cross talk. This cross talk represents a major challenge in high-quality RGB+NIR imaging, rendering existing spatially multiplexed sensor designs impractical. In this work, we introduce a new approach to RGB+NIR image reconstruction using learned convolutional sparse priors. We demonstrate high-quality color and NIR imaging for challenging scenes, even including high-frequency structured NIR illumination. The effectiveness of the proposed method is validated on a large data set of experimental captures, and simulated benchmark results which demonstrate that this work achieves unprecedented reconstruction quality.

Hyperspectral imaging fluorescence excitation scanning for colon cancer detection

PubMed Central

Leavesley, Silas J.; Walters, Mikayla; Lopez, Carmen; Baker, Thomas; Favreau, Peter F.; Rich, Thomas C.; Rider, Paul F.; Boudreaux, Carole W.

2016-01-01

Abstract. Optical spectroscopy and hyperspectral imaging have shown the potential to discriminate between cancerous and noncancerous tissue with high sensitivity and specificity. However, to date, these techniques have not been effectively translated to real-time endoscope platforms. Hyperspectral imaging of the fluorescence excitation spectrum represents new technology that may be well suited for endoscopic implementation. However, the feasibility of detecting differences between normal and cancerous mucosa using fluorescence excitation-scanning hyperspectral imaging has not been evaluated. The goal of this study was to evaluate the initial feasibility of using fluorescence excitation-scanning hyperspectral imaging for measuring changes in fluorescence excitation spectrum concurrent with colonic adenocarcinoma using a small pre-pilot-scale sample size. Ex vivo analysis was performed using resected pairs of colorectal adenocarcinoma and normal mucosa. Adenocarcinoma was confirmed by histologic evaluation of hematoxylin and eosin (H&E) permanent sections. Specimens were imaged using a custom hyperspectral imaging fluorescence excitation-scanning microscope system. Results demonstrated consistent spectral differences between normal and cancerous tissues over the fluorescence excitation range of 390 to 450 nm that could be the basis for wavelength-dependent detection of colorectal cancers. Hence, excitation-scanning hyperspectral imaging may offer an alternative approach for discriminating adenocarcinoma from surrounding normal colonic mucosa, but further studies will be required to evaluate the accuracy of this approach using a larger patient cohort. PMID:27792808

Two-color temporal focusing multiphoton excitation imaging with tunable-wavelength excitation

NASA Astrophysics Data System (ADS)

Lien, Chi-Hsiang; Abrigo, Gerald; Chen, Pei-Hsuan; Chien, Fan-Ching

2017-02-01

Wavelength tunable temporal focusing multiphoton excitation microscopy (TFMPEM) is conducted to visualize optical sectioning images of multiple fluorophore-labeled specimens through the optimal two-photon excitation (TPE) of each type of fluorophore. The tunable range of excitation wavelength was determined by the groove density of the grating, the diffraction angle, the focal length of lenses, and the shifting distance of the first lens in the beam expander. Based on a consideration of the trade-off between the tunable-wavelength range and axial resolution of temporal focusing multiphoton excitation imaging, the presented system demonstrated a tunable-wavelength range from 770 to 920 nm using a diffraction grating with groove density of 830 lines/mm. TPE fluorescence imaging examination of a fluorescent thin film indicated that the width of the axial confined excitation was 3.0±0.7 μm and the shifting distance of the temporal focal plane was less than 0.95 μm within the presented wavelength tunable range. Fast different wavelength excitation and three-dimensionally rendered imaging of Hela cell mitochondria and cytoskeletons and mouse muscle fibers were demonstrated. Significantly, the proposed system can improve the quality of two-color TFMPEM images through different excitation wavelengths to obtain higher-quality fluorescent signals in multiple-fluorophore measurements.

Method and apparatus for imaging a sample on a device

DOEpatents

Trulson, Mark; Stern, David; Fiekowsky, Peter; Rava, Richard; Walton, Ian; Fodor, Stephen P. A.

1996-01-01

The present invention provides methods and systems for detecting a labeled marker on a sample located on a support. The imaging system comprises a body for immobilizing the support, an excitation radiation source and excitation optics to generate and direct the excitation radiation at the sample. In response, labeled material on the sample emits radiation which has a wavelength that is different from the excitation wavelength, which radiation is collected by collection optics and imaged onto a detector which generates an image of the sample.

Sparse Coding and Lateral Inhibition Arising from Balanced and Unbalanced Dendrodendritic Excitation and Inhibition

PubMed Central

Migliore, Michele; Hines, Michael L.; Shepherd, Gordon M.

2014-01-01

The precise mechanism by which synaptic excitation and inhibition interact with each other in odor coding through the unique dendrodendritic synaptic microcircuits present in olfactory bulb is unknown. Here a scaled-up model of the mitral–granule cell network in the rodent olfactory bulb is used to analyze dendrodendritic processing of experimentally determined odor patterns. We found that the interaction between excitation and inhibition is responsible for two fundamental computational mechanisms: (1) a balanced excitation/inhibition in strongly activated mitral cells, leading to a sparse representation of odorant input, and (2) an unbalanced excitation/inhibition (inhibition dominated) in surrounding weakly activated mitral cells, leading to lateral inhibition. These results suggest how both mechanisms can carry information about the input patterns, with optimal level of synaptic excitation and inhibition producing the highest level of sparseness and decorrelation in the network response. The results suggest how the learning process, through the emergent development of these mechanisms, can enhance odor representation of olfactory bulb. PMID:25297097

Odor-evoked inhibition of olfactory sensory neurons drives olfactory perception in Drosophila.

PubMed

Cao, Li-Hui; Yang, Dong; Wu, Wei; Zeng, Xiankun; Jing, Bi-Yang; Li, Meng-Tong; Qin, Shanshan; Tang, Chao; Tu, Yuhai; Luo, Dong-Gen

2017-11-07

Inhibitory response occurs throughout the nervous system, including the peripheral olfactory system. While odor-evoked excitation in peripheral olfactory cells is known to encode odor information, the molecular mechanism and functional roles of odor-evoked inhibition remain largely unknown. Here, we examined Drosophila olfactory sensory neurons and found that inhibitory odors triggered outward receptor currents by reducing the constitutive activities of odorant receptors, inhibiting the basal spike firing in olfactory sensory neurons. Remarkably, this odor-evoked inhibition of olfactory sensory neurons elicited by itself a full range of olfactory behaviors from attraction to avoidance, as did odor-evoked olfactory sensory neuron excitation. These results indicated that peripheral inhibition is comparable to excitation in encoding sensory signals rather than merely regulating excitation. Furthermore, we demonstrated that a bidirectional code with both odor-evoked inhibition and excitation in single olfactory sensory neurons increases the odor-coding capacity, providing a means of efficient sensory encoding.

Designed Er(3+)-singly doped NaYF4 with double excitation bands for simultaneous deep macroscopic and microscopic upconverting bioimaging.

PubMed

Wen, Xuanyuan; Wang, Baoju; Wu, Ruitao; Li, Nana; He, Sailing; Zhan, Qiuqiang

2016-06-01

Simultaneous deep macroscopic imaging and microscopic imaging is in urgent demand, but is challenging to achieve experimentally due to the lack of proper fluorescent probes. Herein, we have designed and successfully synthesized simplex Er(3+)-doped upconversion nanoparticles (UCNPs) with double excitation bands for simultaneous deep macroscopic and microscopic imaging. The material structure and the excitation wavelength of Er(3+)-singly doped UCNPs were further optimized to enhance the upconversion emission efficiency. After optimization, we found that NaYF4:30%Er(3+)@NaYF4:2%Er(3+) could simultaneously achieve efficient two-photon excitation (2PE) macroscopic tissue imaging and three-photon excitation (3PE) deep microscopic when excited by 808 nm continuous wave (CW) and 1480 nm CW lasers, respectively. In vitro cell imaging and in vivo imaging have also been implemented to demonstrate the feasibility and potential of the proposed simplex Er(3+)-doped UCNPs as bioprobe.

Intravital autofluorescence 2-photon microscopy of murine intestinal mucosa with ultra-broadband femtosecond laser pulse excitation: image quality, photodamage, and inflammation

NASA Astrophysics Data System (ADS)

Klinger, Antje; Krapf, Lisa; Orzekowsky-Schroeder, Regina; Koop, Norbert; Vogel, Alfred; Hüttmann, Gereon

2015-11-01

Ultra-broadband excitation with ultrashort pulses may enable simultaneous excitation of multiple endogenous fluorophores in vital tissue. Imaging living gut mucosa by autofluorescence 2-photon microscopy with more than 150 nm broad excitation at an 800-nm central wavelength from a sub-10 fs titanium-sapphire (Ti:sapphire) laser with a dielectric mirror based prechirp was compared to the excitation with 220 fs pulses of a tunable Ti:sapphire laser at 730 and 800 nm wavelengths. Excitation efficiency, image quality, and photochemical damage were evaluated. At similar excitation fluxes, the same image brightness was achieved with both lasers. As expected, with ultra-broadband pulses, fluorescence from NAD(P)H, flavines, and lipoproteins was observed simultaneously. However, nonlinear photodamage apparent as hyperfluorescence with functional and structural alterations of the tissue occurred earlier when the laser power was adjusted to the same image brightness. After only a few minutes, the immigration of polymorphonuclear leucocytes into the epithelium and degranulation of these cells, a sign of inflammation, was observed. Photodamage is promoted by the higher peak irradiances and/or by nonoptimal excitation of autofluorescence at the longer wavelength. We conclude that excitation with a tunable narrow bandwidth laser is preferable to ultra-broadband excitation for autofluorescence-based 2-photon microscopy, unless the spectral phase can be controlled to optimize excitation conditions.

Toward Supersonic Retropropulsion CFD Validation

NASA Technical Reports Server (NTRS)

Kleb, Bil; Schauerhamer, D. Guy; Trumble, Kerry; Sozer, Emre; Barnhardt, Michael; Carlson, Jan-Renee; Edquist, Karl

2011-01-01

This paper begins the process of verifying and validating computational fluid dynamics (CFD) codes for supersonic retropropulsive flows. Four CFD codes (DPLR, FUN3D, OVERFLOW, and US3D) are used to perform various numerical and physical modeling studies toward the goal of comparing predictions with a wind tunnel experiment specifically designed to support CFD validation. Numerical studies run the gamut in rigor from code-to-code comparisons to observed order-of-accuracy tests. Results indicate that this complex flowfield, involving time-dependent shocks and vortex shedding, design order of accuracy is not clearly evident. Also explored is the extent of physical modeling necessary to predict the salient flowfield features found in high-speed Schlieren images and surface pressure measurements taken during the validation experiment. Physical modeling studies include geometric items such as wind tunnel wall and sting mount interference, as well as turbulence modeling that ranges from a RANS (Reynolds-Averaged Navier-Stokes) 2-equation model to DES (Detached Eddy Simulation) models. These studies indicate that tunnel wall interference is minimal for the cases investigated; model mounting hardware effects are confined to the aft end of the model; and sparse grid resolution and turbulence modeling can damp or entirely dissipate the unsteadiness of this self-excited flow.

Calculation of molecular excitation rates

NASA Technical Reports Server (NTRS)

Flynn, George

1993-01-01

State-to-state collisional excitation rates for interstellar molecules observed by radio astronomers continue to be required to interpret observed line intensities in terms of local temperatures and densities. A problem of particular interest is collisional excitation of water which is important for modeling the observed interstellar masers. In earlier work supported by a different NASA Grant, excitation of water in collisions with He atoms was studied; after many years of successively more refined calculations that problem now seems to be well understood, and discrepancies with earlier experimental data for related (pressure broadening) phenomena are believed to reflect experimental errors. Because of interstellar abundances, excitation by H2, the dominant interstellar species, is much more important than excitation by He, although it has been argued that rates for excitation by these are similar. Under the current grant theoretical study of this problem has begun which is greatly complicated by the additional degrees of freedom which must be included both in determining the interaction potential and also in the molecular scattering calculation. We have now computed the interaction forces for nearly a thousand molecular geometries and are close to having an acceptable global fit to these points which is necessary for the molecular dynamics calculations. Also, extensive modifications have been made to the molecular scattering code, MOLSCAT. These included coding the rotational basis sets and coupling matrix elements required for collisions of an asymmetric top with a linear rotor. A new method for numerical solution of the coupled equations has been incorporated. Because of the long-ranged nature of the water-hydrogen interaction it is necessary to integrate the equations to rather large intermolecular separations, and the integration methods previously available in MOLSCAT are not ideal for such cases. However, the method used by Alexander in his HIBRIDON code is particularly suited for such cases. We have obtained this code and incorporated that part which solves the coupled differential equations as an option in the MOLSCAT program.

A System-on-Chip Solution for Point-of-Care Ultrasound Imaging Systems: Architecture and ASIC Implementation.

PubMed

Kang, Jeeun; Yoon, Changhan; Lee, Jaejin; Kye, Sang-Bum; Lee, Yongbae; Chang, Jin Ho; Kim, Gi-Duck; Yoo, Yangmo; Song, Tai-kyong

2016-04-01

In this paper, we present a novel system-on-chip (SOC) solution for a portable ultrasound imaging system (PUS) for point-of-care applications. The PUS-SOC includes all of the signal processing modules (i.e., the transmit and dynamic receive beamformer modules, mid- and back-end processors, and color Doppler processors) as well as an efficient architecture for hardware-based imaging methods (e.g., dynamic delay calculation, multi-beamforming, and coded excitation and compression). The PUS-SOC was fabricated using a UMC 130-nm NAND process and has 16.8 GFLOPS of computing power with a total equivalent gate count of 12.1 million, which is comparable to a Pentium-4 CPU. The size and power consumption of the PUS-SOC are 27×27 mm(2) and 1.2 W, respectively. Based on the PUS-SOC, a prototype hand-held US imaging system was implemented. Phantom experiments demonstrated that the PUS-SOC can provide appropriate image quality for point-of-care applications with a compact PDA size ( 200×120×45 mm(3)) and 3 hours of battery life.

Half radiofrequency pulse excitation with a dedicated prescan to correct eddy current effect and gradient delay.

PubMed

Abe, Takayuki

2013-03-01

To improve the slice profile of the half radiofrequency (RF) pulse excitation and image quality of ultrashort echo time (UTE) imaging by compensating for an eddy current effect. The dedicated prescan has been developed to measure the phase accumulation due to eddy currents induced by the slice-selective gradient. The prescan measures two one-dimensional excitation k-space profiles, which can be acquired with a readout gradient in the slice-selection direction by changing the polarity of the slice-selective gradient. The time shifts due to the phase accumulation in the excitation k-space were calculated. The time shift compensated for the start time of the slice-selective gradient. The total prescan time was 6-15 s. The slice profile and the UTE image with the half RF pulse excitation were acquired to evaluate the slice selectivity and the image quality. Improved slice selectivity was obtained. The simple method proposed in this paper can eliminate eddy current effect. Good UTE images were obtained. The slice profile of the half RF pulse excitation and the image quality of UTE images have been improved by using a dedicated prescan. This method has a possibility that can improve the image quality of a clinical UTE imaging.

Visible-to-visible four-photon ultrahigh resolution microscopic imaging with 730-nm diode laser excited nanocrystals.

PubMed

Wang, Baoju; Zhan, Qiuqiang; Zhao, Yuxiang; Wu, Ruitao; Liu, Jing; He, Sailing

2016-01-25

Further development of multiphoton microscopic imaging is confronted with a number of limitations, including high-cost, high complexity and relatively low spatial resolution due to the long excitation wavelength. To overcome these problems, for the first time, we propose visible-to-visible four-photon ultrahigh resolution microscopic imaging by using a common cost-effective 730-nm laser diode to excite the prepared Nd(3+)-sensitized upconversion nanoparticles (Nd(3+)-UCNPs). An ordinary multiphoton scanning microscope system was built using a visible CW diode laser and the lateral imaging resolution as high as 161-nm was achieved via the four-photon upconversion process. The demonstrated large saturation excitation power for Nd(3+)-UCNPs would be more practical and facilitate the four-photon imaging in the application. A sample with fine structure was imaged to demonstrate the advantages of visible-to-visible four-photon ultrahigh resolution microscopic imaging with 730-nm diode laser excited nanocrystals. Combining the uniqueness of UCNPs, the proposed visible-to-visible four-photon imaging would be highly promising and attractive in the field of multiphoton imaging.

4800 B/S speech compression techniques for mobile satellite systems

NASA Technical Reports Server (NTRS)

Townes, S. A.; Barnwell, T. P., III; Rose, R. C.; Gersho, A.; Davidson, G.

1986-01-01

This paper will discuss three 4800 bps digital speech compression techniques currently being investigated for application in the mobile satellite service. These three techniques, vector adaptive predictive coding, vector excitation coding, and the self excited vocoder, are the most promising among a number of techniques being developed to possibly provide near-toll-quality speech compression while still keeping the bit-rate low enough for a power and bandwidth limited satellite service.

A Bit Stream Scalable Speech/Audio Coder Combining Enhanced Regular Pulse Excitation and Parametric Coding

NASA Astrophysics Data System (ADS)

Riera-Palou, Felip; den Brinker, Albertus C.

2007-12-01

This paper introduces a new audio and speech broadband coding technique based on the combination of a pulse excitation coder and a standardized parametric coder, namely, MPEG-4 high-quality parametric coder. After presenting a series of enhancements to regular pulse excitation (RPE) to make it suitable for the modeling of broadband signals, it is shown how pulse and parametric codings complement each other and how they can be merged to yield a layered bit stream scalable coder able to operate at different points in the quality bit rate plane. The performance of the proposed coder is evaluated in a listening test. The major result is that the extra functionality of the bit stream scalability does not come at the price of a reduced performance since the coder is competitive with standardized coders (MP3, AAC, SSC).

Anticounterfeiting Quick Response Code with Emission Color of Invisible Metal-Organic Frameworks as Encoding Information.

PubMed

Wang, Yong-Mei; Tian, Xue-Tao; Zhang, Hui; Yang, Zhong-Rui; Yin, Xue-Bo

2018-06-21

Counterfeiting is a global epidemic that is compelling the development of new anticounterfeiting strategy. Herein, we report a novel multiple anticounterfeiting encoding strategy of invisible fluorescent quick response (QR) codes with emission color as information storage unit. The strategy requires red, green, and blue (RGB) light-emitting materials for different emission colors as encrypting information, single excitation for all of the emission for practicability, and ultraviolet (UV) excitation for invisibility under daylight. Therefore, RGB light-emitting nanoscale metal-organic frameworks (NMOFs) are designed as inks to construct the colorful light-emitting boxes for information encrypting, while three black vertex boxes were used for positioning. Full-color emissions are obtained by mixing the trichromatic NMOFs inks through inkjet printer. The encrypting information capacity is easily adjusted by the number of light-emitting boxes with the infinite emission colors. The information is decoded with specific excitation light at 275 nm, making the QR codes invisible under daylight. The composition of inks, invisibility, inkjet printing, and the abundant encrypting information all contribute to multiple anticounterfeiting. The proposed QR codes pattern holds great potential for advanced anticounterfeiting.

KEWPIE2: A cascade code for the study of dynamical decay of excited nuclei

NASA Astrophysics Data System (ADS)

Lü, Hongliang; Marchix, Anthony; Abe, Yasuhisa; Boilley, David

2016-03-01

KEWPIE-a cascade code devoted to investigating the dynamical decay of excited nuclei, specially designed for treating very low probability events related to the synthesis of super-heavy nuclei formed in fusion-evaporation reactions-has been improved and rewritten in C++ programming language to become KEWPIE2. The current version of the code comprises various nuclear models concerning the light-particle emission, fission process and statistical properties of excited nuclei. General features of the code, such as the numerical scheme and the main physical ingredients, are described in detail. Some typical calculations having been performed in the present paper clearly show that theoretical predictions are generally in accordance with experimental data. Furthermore, since the values of some input parameters cannot be determined neither theoretically nor experimentally, a sensibility analysis is presented. To this end, we systematically investigate the effects of using different parameter values and reaction models on the final results. As expected, in the case of heavy nuclei, the fission process has the most crucial role to play in theoretical predictions. This work would be essential for numerical modeling of fusion-evaporation reactions.

All-near-infrared multiphoton microscopy interrogates intact tissues at deeper imaging depths than conventional single- and two-photon near-infrared excitation microscopes

PubMed Central

Sarder, Pinaki; Yazdanfar, Siavash; Akers, Walter J.; Tang, Rui; Sudlow, Gail P.; Egbulefu, Christopher

2013-01-01

Abstract. The era of molecular medicine has ushered in the development of microscopic methods that can report molecular processes in thick tissues with high spatial resolution. A commonality in deep-tissue microscopy is the use of near-infrared (NIR) lasers with single- or multiphoton excitations. However, the relationship between different NIR excitation microscopic techniques and the imaging depths in tissue has not been established. We compared such depth limits for three NIR excitation techniques: NIR single-photon confocal microscopy (NIR SPCM), NIR multiphoton excitation with visible detection (NIR/VIS MPM), and all-NIR multiphoton excitation with NIR detection (NIR/NIR MPM). Homologous cyanine dyes provided the fluorescence. Intact kidneys were harvested after administration of kidney-clearing cyanine dyes in mice. NIR SPCM and NIR/VIS MPM achieved similar maximum imaging depth of ∼100  μm. The NIR/NIR MPM enabled greater than fivefold imaging depth (>500  μm) using the harvested kidneys. Although the NIR/NIR MPM used 1550-nm excitation where water absorption is relatively high, cell viability and histology studies demonstrate that the laser did not induce photothermal damage at the low laser powers used for the kidney imaging. This study provides guidance on the imaging depth capabilities of NIR excitation-based microscopic techniques and reveals the potential to multiplex information using these platforms. PMID:24150231

Spectroscopic imaging of self-organization in high power impulse magnetron sputtering plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Andersson, Joakim; Centre for Quantum Technologies, National University of Singapore, 3 Science Drive 2, 117543 Singapore; Ni, Pavel

Excitation and ionization conditions in traveling ionization zones of high power impulse magnetron sputtering plasmas were investigated using fast camera imaging through interference filters. The images, taken in end-on and side-on views using light of selected gas and target atom and ion spectral lines, suggest that ionization zones are regions of enhanced densities of electrons, and excited atoms and ions. Excited atoms and ions of the target material (Al) are strongly concentrated near the target surface. Images from the highest excitation energies exhibit the most localized regions, suggesting localized Ohmic heating consistent with double layer formation.

Spectroscopic imaging of self-organization in high power impulse magnetron sputtering plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Centre for Quantum Technologies, National University of Singapore, 3 Science Drive 2, 117543 Singapore, Singapore; Andersson, Joakim; Ni, Pavel

Excitation and ionization conditions in traveling ionization zones of high power impulse magnetron sputtering plasmas were investigated using fast camera imaging through interference filters. The images, taken in end-on and side on views using light of selected gas and target atom and ion spectral lines, suggest that ionization zones are regions of enhanced densities of electrons, and excited atoms and ions. Excited atoms and ions of the target material (Al) are strongly concentrated near the target surface. Images from the highest excitation energies exhibit the most localized regions, suggesting localized Ohmic heating consistent with double layer formation.

Vector Sum Excited Linear Prediction (VSELP) speech coding at 4.8 kbps

NASA Technical Reports Server (NTRS)

Gerson, Ira A.; Jasiuk, Mark A.

1990-01-01

Code Excited Linear Prediction (CELP) speech coders exhibit good performance at data rates as low as 4800 bps. The major drawback to CELP type coders is their larger computational requirements. The Vector Sum Excited Linear Prediction (VSELP) speech coder utilizes a codebook with a structure which allows for a very efficient search procedure. Other advantages of the VSELP codebook structure is discussed and a detailed description of a 4.8 kbps VSELP coder is given. This coder is an improved version of the VSELP algorithm, which finished first in the NSA's evaluation of the 4.8 kbps speech coders. The coder uses a subsample resolution single tap long term predictor, a single VSELP excitation codebook, a novel gain quantizer which is robust to channel errors, and a new adaptive pre/postfilter arrangement.

Excitation-scanning hyperspectral imaging as a means to discriminate various tissues types

NASA Astrophysics Data System (ADS)

Deal, Joshua; Favreau, Peter F.; Lopez, Carmen; Lall, Malvika; Weber, David S.; Rich, Thomas C.; Leavesley, Silas J.

2017-02-01

Little is currently known about the fluorescence excitation spectra of disparate tissues and how these spectra change with pathological state. Current imaging diagnostic techniques have limited capacity to investigate fluorescence excitation spectral characteristics. This study utilized excitation-scanning hyperspectral imaging to perform a comprehensive assessment of fluorescence spectral signatures of various tissues. Immediately following tissue harvest, a custom inverted microscope (TE-2000, Nikon Instruments) with Xe arc lamp and thin film tunable filter array (VersaChrome, Semrock, Inc.) were used to acquire hyperspectral image data from each sample. Scans utilized excitation wavelengths from 340 nm to 550 nm in 5 nm increments. Hyperspectral images were analyzed with custom Matlab scripts including linear spectral unmixing (LSU), principal component analysis (PCA), and Gaussian mixture modeling (GMM). Spectra were examined for potential characteristic features such as consistent intensity peaks at specific wavelengths or intensity ratios among significant wavelengths. The resultant spectral features were conserved among tissues of similar molecular composition. Additionally, excitation spectra appear to be a mixture of pure endmembers with commonalities across tissues of varied molecular composition, potentially identifiable through GMM. These results suggest the presence of common autofluorescent molecules in most tissues and that excitationscanning hyperspectral imaging may serve as an approach for characterizing tissue composition as well as pathologic state. Future work will test the feasibility of excitation-scanning hyperspectral imaging as a contrast mode for discriminating normal and pathological tissues.

Vibration Response Models of a Stiffened Aluminum Plate Excited by a Shaker

NASA Technical Reports Server (NTRS)

Cabell, Randolph H.

2008-01-01

Numerical models of structural-acoustic interactions are of interest to aircraft designers and the space program. This paper describes a comparison between two energy finite element codes, a statistical energy analysis code, a structural finite element code, and the experimentally measured response of a stiffened aluminum plate excited by a shaker. Different methods for modeling the stiffeners and the power input from the shaker are discussed. The results show that the energy codes (energy finite element and statistical energy analysis) accurately predicted the measured mean square velocity of the plate. In addition, predictions from an energy finite element code had the best spatial correlation with measured velocities. However, predictions from a considerably simpler, single subsystem, statistical energy analysis model also correlated well with the spatial velocity distribution. The results highlight a need for further work to understand the relationship between modeling assumptions and the prediction results.

Compressive Coded-Aperture Multimodal Imaging Systems

NASA Astrophysics Data System (ADS)

Rueda-Chacon, Hoover F.

Multimodal imaging refers to the framework of capturing images that span different physical domains such as space, spectrum, depth, time, polarization, and others. For instance, spectral images are modeled as 3D cubes with two spatial and one spectral coordinate. Three-dimensional cubes spanning just the space domain, are referred as depth volumes. Imaging cubes varying in time, spectra or depth, are referred as 4D-images. Nature itself spans different physical domains, thus imaging our real world demands capturing information in at least 6 different domains simultaneously, giving turn to 3D-spatial+spectral+polarized dynamic sequences. Conventional imaging devices, however, can capture dynamic sequences with up-to 3 spectral channels, in real-time, by the use of color sensors. Capturing multiple spectral channels require scanning methodologies, which demand long time. In general, to-date multimodal imaging requires a sequence of different imaging sensors, placed in tandem, to simultaneously capture the different physical properties of a scene. Then, different fusion techniques are employed to mix all the individual information into a single image. Therefore, new ways to efficiently capture more than 3 spectral channels of 3D time-varying spatial information, in a single or few sensors, are of high interest. Compressive spectral imaging (CSI) is an imaging framework that seeks to optimally capture spectral imagery (tens of spectral channels of 2D spatial information), using fewer measurements than that required by traditional sensing procedures which follows the Shannon-Nyquist sampling. Instead of capturing direct one-to-one representations of natural scenes, CSI systems acquire linear random projections of the scene and then solve an optimization algorithm to estimate the 3D spatio-spectral data cube by exploiting the theory of compressive sensing (CS). To date, the coding procedure in CSI has been realized through the use of ``block-unblock" coded apertures, commonly implemented as chrome-on-quartz photomasks. These apertures block or permit to pass the entire spectrum from the scene at given spatial locations, thus modulating the spatial characteristics of the scene. In the first part, this thesis aims to expand the framework of CSI by replacing the traditional block-unblock coded apertures by patterned optical filter arrays, referred as ``color" coded apertures. These apertures are formed by tiny pixelated optical filters, which in turn, allow the input image to be modulated not only spatially but spectrally as well, entailing more powerful coding strategies. The proposed colored coded apertures are either synthesized through linear combinations of low-pass, high-pass and band-pass filters, paired with binary pattern ensembles realized by a digital-micromirror-device (DMD), or experimentally realized through thin-film color-patterned filter arrays. The optical forward model of the proposed CSI architectures will be presented along with the design and proof-of-concept implementations, which achieve noticeable improvements in the quality of the reconstructions compared with conventional block-unblock coded aperture-based CSI architectures. On another front, due to the rich information contained in the infrared spectrum as well as the depth domain, this thesis aims to explore multimodal imaging by extending the range sensitivity of current CSI systems to a dual-band visible+near-infrared spectral domain, and also, it proposes, for the first time, a new imaging device that captures simultaneously 4D data cubes (2D spatial+1D spectral+depth imaging) with as few as a single snapshot. Due to the snapshot advantage of this camera, video sequences are possible, thus enabling the joint capture of 5D imagery. It aims to create super-human sensing that will enable the perception of our world in new and exciting ways. With this, we intend to advance in the state of the art in compressive sensing systems to extract depth while accurately capturing spatial and spectral material properties. The applications of such a sensor are self-evident in fields such as computer/robotic vision because they would allow an artificial intelligence to make informed decisions about not only the location of objects within a scene but also their material properties.

Pulse compression of harmonic chirp signals using the fractional fourier transform.

PubMed

Arif, M; Cowell, D M J; Freear, S

2010-06-01

In ultrasound harmonic imaging with chirp-coded excitation, a harmonic matched filter (HMF) is typically used on the received signal to perform pulse compression of the second harmonic component (SHC) to recover signal axial resolution. Designing the HMF for the compression of the SHC is a problematic issue because it requires optimal window selection. In the compressed second harmonic signal, the sidelobe level may increase and the mainlobe width (MLW) widen under a mismatched condition, resulting in loss of axial resolution. We propose the use of the fractional Fourier transform (FrFT) as an alternative tool to perform compression of the chirp-coded SHC generated as a result of the nonlinear propagation of an ultrasound signal. Two methods are used to experimentally assess the performance benefits of the FrFT technique over the HMF techniques. The first method uses chirp excitation with central frequency of 2.25 MHz and bandwidth of 1 MHz. The second method uses chirp excitation with pulse inversion to increase the bandwidth to 2 MHz. In this study, experiments were performed in a water tank with a single-element transducer mounted coaxially with a hydrophone in a pitch-catch configuration. Results are presented that indicate that the FrFT can perform pulse compression of the second harmonic chirp component, with a 14% reduction in the MLW of the compressed signal when compared with the HMF. Also, the FrFT provides at least 23% reduction in the MLW of the compressed signal when compared with the harmonic mismatched filter (HMMF). The FrFT maintains comparable peak and integrated sidelobe levels when compared with the HMF and HMMF techniques. Copyright 2010 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

An integrated single- and two-photon non-diffracting light-sheet microscope

NASA Astrophysics Data System (ADS)

Lau, Sze Cheung; Chiu, Hoi Chun; Zhao, Luwei; Zhao, Teng; Loy, M. M. T.; Du, Shengwang

2018-04-01

We describe a fluorescence optical microscope with both single-photon and two-photon non-diffracting light-sheet excitations for large volume imaging. With a special design to accommodate two different wavelength ranges (visible: 400-700 nm and near infrared: 800-1200 nm), we combine the line-Bessel sheet (LBS, for single-photon excitation) and the scanning Bessel beam (SBB, for two-photon excitation) light sheet together in a single microscope setup. For a transparent thin sample where the scattering can be ignored, the LBS single-photon excitation is the optimal imaging solution. When the light scattering becomes significant for a deep-cell or deep-tissue imaging, we use SBB light-sheet two-photon excitation with a longer wavelength. We achieved nearly identical lateral/axial resolution of about 350/270 nm for both imagings. This integrated light-sheet microscope may have a wide application for live-cell and live-tissue three-dimensional high-speed imaging.

OH and CH luminescence in opposed flow methane oxy-flames

DOE Office of Scientific and Technical Information (OSTI.GOV)

De Leo, Maurizio; Saveliev, Alexei; Kennedy, Lawrence A.

Emission spectroscopy is a 2-D nonintrusive diagnostic technique that offers spatially resolved data for combustion optimization and control. The UV and visible chemiluminescence of the excited radicals CH(A{sup 2}{delta},B{sup 2}{sigma}{sup -}) and OH(A{sup 2}{sigma}{sup +}) is studied experimentally and numerically in opposed-flow diffusion flames of methane and oxygen-enriched air. The oxidized oxygen content is varied from 21 to 100% while the range of the studied strain rates spans from 20 to 40 s{sup -1}. The spectrally resolved imaging is obtained by two different methods: scattering through a grating monochromator and interposition of interference filters along the optical path. Absolute measuredmore » chemiluminescence intensities, coupled with a numerical model based on the opposed flow flame code, are used to evaluate the chemical kinetics of the excited species. The predictions of the selected model are in good agreement with the experimental data over the range of the studied flame conditions. (author)« less

MHD control experiments in the Extrap T2R Reversed Field Pinch

NASA Astrophysics Data System (ADS)

Marrelli, L.; Bolzonella, T.; Brunsell, P.; Cecconello, M.; Drake, J.; Franz, P.; Gregoratto, D.; Manduchi, G.; Martin, P.; Ortolani, S.; Paccagnella, R.; Piovesan, P.; Spizzo, G.; Yadikin, D.; Zanca, P.

2004-11-01

We report here on MHD active control experiments performed in the Extrap T2R device, which has been recently equipped with a set of 32 feedback controlled saddle coils couples. Experiments aiming at selectively exciting a resonant resistive instability in order to actively induce Quasi Single Helicity states will be presented. Open loop experiments have in fact shown that a spectrum with one dominant mode can be excited in a high aspect ratio device like T2R. In addition, evidences of controlled braking of tearing modes, which spontaneously rotate in T2R, have been gathered, allowing the determination of a threshold for mode wall locking. Different feedback control schemes have been implemented. In particular, mode suppression schemes proved successful in delaying resistive wall modes growth and in increasing the discharge duration: this suggests a hybrid mode control scenario, in which RWM are suppressed and QSH is induced. Radiation imaging and internal magnetic field reconstructions performed with the ORBIT code will be presented.

Jetting of a ultrasound contrast microbubble near a rigid wall

NASA Astrophysics Data System (ADS)

Sarkar, Kausik; Mobadersany, Nima

2017-11-01

Micron sized gas-bubbles coated with a stabilizing shell of lipids or proteins, are used as contrast enhancing agents for ultrasound imaging. However, they are increasingly being explored for novel applications in drug delivery through a process called sonoporation, the reversible permeabilization of the cell membrane. Under sufficiently strong acoustic excitations, bubbles form a jet and collapse near a wall. The jetting of free bubbles has been extensively studied by boundary element method (BEM). Here, for the first time, we implemented a rigorous interfacial rheological model of the shell into BEM and investigated the jet formation. The code has been carefully validated against past results. Increasing shell elasticity decreases the maximum bubble volume and the collapse time, while the jet velocity increases. The shear stress on the wall is computed and analyzed. A phase diagram as functions of excitation pressure and wall separation describes jet formation. Effects of shell elasticity and frequency on the phase diagram are investigated. Partially supported by National Science Foundation.

An excitation wavelength-scanning spectral imaging system for preclinical imaging

NASA Astrophysics Data System (ADS)

Leavesley, Silas; Jiang, Yanan; Patsekin, Valery; Rajwa, Bartek; Robinson, J. Paul

2008-02-01

Small-animal fluorescence imaging is a rapidly growing field, driven by applications in cancer detection and pharmaceutical therapies. However, the practical use of this imaging technology is limited by image-quality issues related to autofluorescence background from animal tissues, as well as attenuation of the fluorescence signal due to scatter and absorption. To combat these problems, spectral imaging and analysis techniques are being employed to separate the fluorescence signal from background autofluorescence. To date, these technologies have focused on detecting the fluorescence emission spectrum at a fixed excitation wavelength. We present an alternative to this technique, an imaging spectrometer that detects the fluorescence excitation spectrum at a fixed emission wavelength. The advantages of this approach include increased available information for discrimination of fluorescent dyes, decreased optical radiation dose to the animal, and ability to scan a continuous wavelength range instead of discrete wavelength sampling. This excitation-scanning imager utilizes an acousto-optic tunable filter (AOTF), with supporting optics, to scan the excitation spectrum. Advanced image acquisition and analysis software has also been developed for classification and unmixing of the spectral image sets. Filtering has been implemented in a single-pass configuration with a bandwidth (full width at half maximum) of 16nm at 550nm central diffracted wavelength. We have characterized AOTF filtering over a wide range of incident light angles, much wider than has been previously reported in the literature, and we show how changes in incident light angle can be used to attenuate AOTF side lobes and alter bandwidth. A new parameter, in-band to out-of-band ratio, was defined to assess the quality of the filtered excitation light. Additional parameters were measured to allow objective characterization of the AOTF and the imager as a whole. This is necessary for comparing the excitation-scanning imager to other spectral and fluorescence imaging technologies. The effectiveness of the hyperspectral imager was tested by imaging and analysis of mice with injected fluorescent dyes. Finally, a discussion of the optimization of spectral fluorescence imagers is given, relating the effects of filter quality on fluorescence images collected and the analysis outcome.

Real-time speech encoding based on Code-Excited Linear Prediction (CELP)

NASA Technical Reports Server (NTRS)

Leblanc, Wilfrid P.; Mahmoud, S. A.

1988-01-01

This paper reports on the work proceeding with regard to the development of a real-time voice codec for the terrestrial and satellite mobile radio environments. The codec is based on a complexity reduced version of code-excited linear prediction (CELP). The codebook search complexity was reduced to only 0.5 million floating point operations per second (MFLOPS) while maintaining excellent speech quality. Novel methods to quantize the residual and the long and short term model filters are presented.

Use of image analysis to estimate anthocyanin and UV-excited fluorescent phenolic compound levels in strawberry fruit

PubMed Central

Yoshioka, Yosuke; Nakayama, Masayoshi; Noguchi, Yuji; Horie, Hideki

2013-01-01

Strawberry is rich in anthocyanins, which are responsible for the red color, and contains several colorless phenolic compounds. Among the colorless phenolic compounds, some, such as hydroxycinammic acid derivatives, emit blue-green fluorescence when excited with ultraviolet (UV) light. Here, we investigated the effectiveness of image analyses for estimating the levels of anthocyanins and UV-excited fluorescent phenolic compounds in fruit. The fruit skin and cut surface of 12 cultivars were photographed under visible and UV light conditions; colors were evaluated based on the color components of images. The levels of anthocyanins and UV-excited fluorescent compounds in each fruit were also evaluated by spectrophotometric and high performance liquid chromatography (HPLC) analyses, respectively and relationships between these levels and the image data were investigated. Red depth of the fruits differed greatly among the cultivars and anthocyanin content was well estimated based on the color values of the cut surface images. Strong UV-excited fluorescence was observed on the cut surfaces of several cultivars, and the grayscale values of the UV-excited fluorescence images were markedly correlated with the levels of those fluorescent compounds as evaluated by HPLC analysis. These results indicate that image analyses can select promising genotypes rich in anthocyanins and fluorescent phenolic compounds. PMID:23853516

Hyperspectral small animal fluorescence imaging: spectral selection imaging

NASA Astrophysics Data System (ADS)

Leavesley, Silas; Jiang, Yanan; Patsekin, Valery; Hall, Heidi; Vizard, Douglas; Robinson, J. Paul

2008-02-01

Molecular imaging is a rapidly growing area of research, fueled by needs in pharmaceutical drug-development for methods for high-throughput screening, pre-clinical and clinical screening for visualizing tumor growth and drug targeting, and a growing number of applications in the molecular biology fields. Small animal fluorescence imaging employs fluorescent probes to target molecular events in vivo, with a large number of molecular targeting probes readily available. The ease at which new targeting compounds can be developed, the short acquisition times, and the low cost (compared to microCT, MRI, or PET) makes fluorescence imaging attractive. However, small animal fluorescence imaging suffers from high optical scattering, absorption, and autofluorescence. Much of these problems can be overcome through multispectral imaging techniques, which collect images at different fluorescence emission wavelengths, followed by analysis, classification, and spectral deconvolution methods to isolate signals from fluorescence emission. We present an alternative to the current method, using hyperspectral excitation scanning (spectral selection imaging), a technique that allows excitation at any wavelength in the visible and near-infrared wavelength range. In many cases, excitation imaging may be more effective at identifying specific fluorescence signals because of the higher complexity of the fluorophore excitation spectrum. Because the excitation is filtered and not the emission, the resolution limit and image shift imposed by acousto-optic tunable filters have no effect on imager performance. We will discuss design of the imager, optimizing the imager for use in small animal fluorescence imaging, and application of spectral analysis and classification methods for identifying specific fluorescence signals.

Defect imaging for plate-like structures using diffuse field.

PubMed

Hayashi, Takahiro

2018-04-01

Defect imaging utilizing a scanning laser source (SLS) technique produces images of defects in a plate-like structure, as well as spurious images occurring because of resonances and reverberations within the specimen. This study developed defect imaging by the SLS using diffuse field concepts to reduce the intensity of spurious images, by which the energy of flexural waves excited by laser can be estimated. The experimental results in the different frequency bandwidths of excitation waves and in specimens with different attenuation proved that clearer images of defects are obtained in broadband excitation using a chirp wave and in specimens with low attenuation, which produce diffuse fields easily.

Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes

PubMed Central

Villa, Carlo E.; Caccia, Michele; Sironi, Laura; D'Alfonso, Laura; Collini, Maddalena; Rivolta, Ilaria; Miserocchi, Giuseppe; Gorletta, Tatiana; Zanoni, Ivan; Granucci, Francesca; Chirico, Giuseppe

2010-01-01

The basic research in cell biology and in medical sciences makes large use of imaging tools mainly based on confocal fluorescence and, more recently, on non-linear excitation microscopy. Substantially the aim is the recognition of selected targets in the image and their tracking in time. We have developed a particle tracking algorithm optimized for low signal/noise images with a minimum set of requirements on the target size and with no a priori knowledge of the type of motion. The image segmentation, based on a combination of size sensitive filters, does not rely on edge detection and is tailored for targets acquired at low resolution as in most of the in-vivo studies. The particle tracking is performed by building, from a stack of Accumulative Difference Images, a single 2D image in which the motion of the whole set of the particles is coded in time by a color level. This algorithm, tested here on solid-lipid nanoparticles diffusing within cells and on lymphocytes diffusing in lymphonodes, appears to be particularly useful for the cellular and the in-vivo microscopy image processing in which few a priori assumption on the type, the extent and the variability of particle motions, can be done. PMID:20808918

Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.

PubMed

Villa, Carlo E; Caccia, Michele; Sironi, Laura; D'Alfonso, Laura; Collini, Maddalena; Rivolta, Ilaria; Miserocchi, Giuseppe; Gorletta, Tatiana; Zanoni, Ivan; Granucci, Francesca; Chirico, Giuseppe

2010-08-17

The basic research in cell biology and in medical sciences makes large use of imaging tools mainly based on confocal fluorescence and, more recently, on non-linear excitation microscopy. Substantially the aim is the recognition of selected targets in the image and their tracking in time. We have developed a particle tracking algorithm optimized for low signal/noise images with a minimum set of requirements on the target size and with no a priori knowledge of the type of motion. The image segmentation, based on a combination of size sensitive filters, does not rely on edge detection and is tailored for targets acquired at low resolution as in most of the in-vivo studies. The particle tracking is performed by building, from a stack of Accumulative Difference Images, a single 2D image in which the motion of the whole set of the particles is coded in time by a color level. This algorithm, tested here on solid-lipid nanoparticles diffusing within cells and on lymphocytes diffusing in lymphonodes, appears to be particularly useful for the cellular and the in-vivo microscopy image processing in which few a priori assumption on the type, the extent and the variability of particle motions, can be done.

Positron follow-up in liquid water: I. A new Monte Carlo track-structure code.

PubMed

Champion, C; Le Loirec, C

2006-04-07

When biological matter is irradiated by charged particles, a wide variety of interactions occur, which lead to a deep modification of the cellular environment. To understand the fine structure of the microscopic distribution of energy deposits, Monte Carlo event-by-event simulations are particularly suitable. However, the development of these track-structure codes needs accurate interaction cross sections for all the electronic processes: ionization, excitation, positronium formation and even elastic scattering. Under these conditions, we have recently developed a Monte Carlo code for positrons in water, the latter being commonly used to simulate the biological medium. All the processes are studied in detail via theoretical differential and total cross-section calculations performed by using partial wave methods. Comparisons with existing theoretical and experimental data in terms of stopping powers, mean energy transfers and ranges show very good agreements. Moreover, thanks to the theoretical description of positronium formation, we have access, for the first time, to the complete kinematics of the electron capture process. Then, the present Monte Carlo code is able to describe the detailed positronium history, which will provide useful information for medical imaging (like positron emission tomography) where improvements are needed to define with the best accuracy the tumoural volumes.

Excitation-scanning hyperspectral imaging microscope

PubMed Central

Favreau, Peter F.; Hernandez, Clarissa; Heaster, Tiffany; Alvarez, Diego F.; Rich, Thomas C.; Prabhat, Prashant; Leavesley, Silas J.

2014-01-01

Abstract. Hyperspectral imaging is a versatile tool that has recently been applied to a variety of biomedical applications, notably live-cell and whole-tissue signaling. Traditional hyperspectral imaging approaches filter the fluorescence emission over a broad wavelength range while exciting at a single band. However, these emission-scanning approaches have shown reduced sensitivity due to light attenuation from spectral filtering. Consequently, emission scanning has limited applicability for time-sensitive studies and photosensitive applications. In this work, we have developed an excitation-scanning hyperspectral imaging microscope that overcomes these limitations by providing high transmission with short acquisition times. This is achieved by filtering the fluorescence excitation rather than the emission. We tested the efficacy of the excitation-scanning microscope in a side-by-side comparison with emission scanning for detection of green fluorescent protein (GFP)-expressing endothelial cells in highly autofluorescent lung tissue. Excitation scanning provided higher signal-to-noise characteristics, as well as shorter acquisition times (300  ms/wavelength band with excitation scanning versus 3  s/wavelength band with emission scanning). Excitation scanning also provided higher delineation of nuclear and cell borders, and increased identification of GFP regions in highly autofluorescent tissue. These results demonstrate excitation scanning has utility in a wide range of time-dependent and photosensitive applications. PMID:24727909

Excitation-scanning hyperspectral imaging microscope.

PubMed

Favreau, Peter F; Hernandez, Clarissa; Heaster, Tiffany; Alvarez, Diego F; Rich, Thomas C; Prabhat, Prashant; Leavesley, Silas J

2014-04-01

Hyperspectral imaging is a versatile tool that has recently been applied to a variety of biomedical applications, notably live-cell and whole-tissue signaling. Traditional hyperspectral imaging approaches filter the fluorescence emission over a broad wavelength range while exciting at a single band. However, these emission-scanning approaches have shown reduced sensitivity due to light attenuation from spectral filtering. Consequently, emission scanning has limited applicability for time-sensitive studies and photosensitive applications. In this work, we have developed an excitation-scanning hyperspectral imaging microscope that overcomes these limitations by providing high transmission with short acquisition times. This is achieved by filtering the fluorescence excitation rather than the emission. We tested the efficacy of the excitation-scanning microscope in a side-by-side comparison with emission scanning for detection of green fluorescent protein (GFP)-expressing endothelial cells in highly autofluorescent lung tissue. Excitation scanning provided higher signal-to-noise characteristics, as well as shorter acquisition times (300  ms/wavelength band with excitation scanning versus 3  s/wavelength band with emission scanning). Excitation scanning also provided higher delineation of nuclear and cell borders, and increased identification of GFP regions in highly autofluorescent tissue. These results demonstrate excitation scanning has utility in a wide range of time-dependent and photosensitive applications.

Photoelectron imaging of doped helium nanodroplets

NASA Astrophysics Data System (ADS)

Neumark, Daniel

2008-03-01

Photoelectron images of helium nanodroplets doped with Kr and Ne atoms are reported. The images and resulting photoelectron spectra were obtained using tunable synchrotron radiation to ionize the droplets. Droplets were excited at 21.6 eV, corresponding to a strong droplet electronic excitation. The rare gas dopant is then ionized via a Penning excitation transfer process. The electron kinetic energy distributions reflect complex ionization and electron escape dynamics.

High dynamic range coding imaging system

NASA Astrophysics Data System (ADS)

Wu, Renfan; Huang, Yifan; Hou, Guangqi

2014-10-01

We present a high dynamic range (HDR) imaging system design scheme based on coded aperture technique. This scheme can help us obtain HDR images which have extended depth of field. We adopt Sparse coding algorithm to design coded patterns. Then we utilize the sensor unit to acquire coded images under different exposure settings. With the guide of the multiple exposure parameters, a series of low dynamic range (LDR) coded images are reconstructed. We use some existing algorithms to fuse and display a HDR image by those LDR images. We build an optical simulation model and get some simulation images to verify the novel system.

Tailoring dye-sensitized upconversion nanoparticle excitation bands towards excitation wavelength selective imaging

DOE PAGES

Wu, Xiang; Lee, Hyungseok; Bilsel, Osman; ...

2015-01-01

One of the key roadblocks in UCNP development is its extremely limited choices of excitation wavelengths. We report a generic design to program UCNPs to possess highly tunable dye characteristic excitation bands. Using such distinctive properties, we were able to develop a new excitation wavelength selective security imaging. Finally, this work unleashed the greater freedom of the excitation wavelengths of the upconversion nanoparticles and we believe it is a game-changer in the field and this method will enable numerous applications that are currently limited by existing UCNPs.

Visual pattern image sequence coding

NASA Technical Reports Server (NTRS)

Silsbee, Peter; Bovik, Alan C.; Chen, Dapang

1990-01-01

The visual pattern image coding (VPIC) configurable digital image-coding process is capable of coding with visual fidelity comparable to the best available techniques, at compressions which (at 30-40:1) exceed all other technologies. These capabilities are associated with unprecedented coding efficiencies; coding and decoding operations are entirely linear with respect to image size and entail a complexity that is 1-2 orders of magnitude faster than any previous high-compression technique. The visual pattern image sequence coding to which attention is presently given exploits all the advantages of the static VPIC in the reduction of information from an additional, temporal dimension, to achieve unprecedented image sequence coding performance.

Co-registered Topographical, Band Excitation Nanomechanical, and Mass Spectral Imaging Using a Combined Atomic Force Microscopy/Mass Spectrometry Platform

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ovchinnikova, Olga S.; Tai, Tamin; Bocharova, Vera

The advancement of a hybrid atomic force microscopy/mass spectrometry imaging platform demonstrating for the first time co-registered topographical, band excitation nanomechanical, and mass spectral imaging of a surface using a single instrument is reported. The mass spectrometry-based chemical imaging component of the system utilized nanothermal analysis probes for pyrolytic surface sampling followed by atmospheric pressure chemical ionization of the gas phase species produced with subsequent mass analysis. We discuss the basic instrumental setup and operation and the multimodal imaging capability and utility are demonstrated using a phase separated polystyrene/poly(2-vinylpyridine) polymer blend thin film. The topography and band excitation images showedmore » that the valley and plateau regions of the thin film surface were comprised primarily of one of the two polymers in the blend with the mass spectral chemical image used to definitively identify the polymers at the different locations. Data point pixel size for the topography (390 nm x 390 nm), band excitation (781 nm x 781 nm), mass spectrometry (690 nm x 500 nm) images was comparable and submicrometer in all three cases, but the data voxel size for each of the three images was dramatically different. The topography image was uniquely a surface measurement, whereas the band excitation image included information from an estimated 10 nm deep into the sample and the mass spectral image from 110-140 nm in depth. Moreover, because of this dramatic sampling depth variance, some differences in the band excitation and mass spectrometry chemical images were observed and were interpreted to indicate the presence of a buried interface in the sample. The spatial resolution of the mass spectral image was estimated to be between 1.5 m 2.6 m, based on the ability to distinguish surface features in that image that were also observed in the other images.« less

Co-registered Topographical, Band Excitation Nanomechanical, and Mass Spectral Imaging Using a Combined Atomic Force Microscopy/Mass Spectrometry Platform

DOE PAGES

Ovchinnikova, Olga S.; Tai, Tamin; Bocharova, Vera; ...

2015-03-18

The advancement of a hybrid atomic force microscopy/mass spectrometry imaging platform demonstrating for the first time co-registered topographical, band excitation nanomechanical, and mass spectral imaging of a surface using a single instrument is reported. The mass spectrometry-based chemical imaging component of the system utilized nanothermal analysis probes for pyrolytic surface sampling followed by atmospheric pressure chemical ionization of the gas phase species produced with subsequent mass analysis. We discuss the basic instrumental setup and operation and the multimodal imaging capability and utility are demonstrated using a phase separated polystyrene/poly(2-vinylpyridine) polymer blend thin film. The topography and band excitation images showedmore » that the valley and plateau regions of the thin film surface were comprised primarily of one of the two polymers in the blend with the mass spectral chemical image used to definitively identify the polymers at the different locations. Data point pixel size for the topography (390 nm x 390 nm), band excitation (781 nm x 781 nm), mass spectrometry (690 nm x 500 nm) images was comparable and submicrometer in all three cases, but the data voxel size for each of the three images was dramatically different. The topography image was uniquely a surface measurement, whereas the band excitation image included information from an estimated 10 nm deep into the sample and the mass spectral image from 110-140 nm in depth. Moreover, because of this dramatic sampling depth variance, some differences in the band excitation and mass spectrometry chemical images were observed and were interpreted to indicate the presence of a buried interface in the sample. The spatial resolution of the mass spectral image was estimated to be between 1.5 m 2.6 m, based on the ability to distinguish surface features in that image that were also observed in the other images.« less

Development of a new EMP code at LANL

NASA Astrophysics Data System (ADS)

Colman, J. J.; Roussel-Dupré, R. A.; Symbalisty, E. M.; Triplett, L. A.; Travis, B. J.

2006-05-01

A new code for modeling the generation of an electromagnetic pulse (EMP) by a nuclear explosion in the atmosphere is being developed. The source of the EMP is the Compton current produced by the prompt radiation (γ-rays, X-rays, and neutrons) of the detonation. As a first step in building a multi- dimensional EMP code we have written three kinetic codes, Plume, Swarm, and Rad. Plume models the transport of energetic electrons in air. The Plume code solves the relativistic Fokker-Planck equation over a specified energy range that can include ~ 3 keV to 50 MeV and computes the resulting electron distribution function at each cell in a two dimensional spatial grid. The energetic electrons are allowed to transport, scatter, and experience Coulombic drag. Swarm models the transport of lower energy electrons in air, spanning 0.005 eV to 30 keV. The swarm code performs a full 2-D solution to the Boltzmann equation for electrons in the presence of an applied electric field. Over this energy range the relevant processes to be tracked are elastic scattering, three body attachment, two body attachment, rotational excitation, vibrational excitation, electronic excitation, and ionization. All of these occur due to collisions between the electrons and neutral bodies in air. The Rad code solves the full radiation transfer equation in the energy range of 1 keV to 100 MeV. It includes effects of photo-absorption, Compton scattering, and pair-production. All of these codes employ a spherical coordinate system in momentum space and a cylindrical coordinate system in configuration space. The "z" axis of the momentum and configuration spaces is assumed to be parallel and we are currently also assuming complete spatial symmetry around the "z" axis. Benchmarking for each of these codes will be discussed as well as the way forward towards an integrated modern EMP code.

Analysis of parasitic oscillations in 42 GHz gyrotron beam tunnel

NASA Astrophysics Data System (ADS)

Kumar, N.; Singh, U.; Singh, T. P.; Sinha, A. K.

2011-02-01

Parasitic oscillation excitation analysis has been carried out for the 42 GHz gyrotron beam tunnel. This article presents a systematic approach for the analysis of parasitic oscillation excitation. The electron trajectory code EGUN has been used for the estimation of the electron beam parameters in the beam tunnel. The electromagnetic simulation code CST-MS has been used for the eigenmode and Q value analysis. The analysis of the parasitic oscillations has been performed for the symmetric TE modes and the first three cavity side copper rings. Four different approaches- the Q value study, the mode maxima-electron beam radius mismatching, the electron cyclotron frequency-mode excitation frequency mismatching and the backward wave interaction analysis- have been used for the parasitic oscillation analysis.

Orientation-dependent imaging of electronically excited quantum dots

NASA Astrophysics Data System (ADS)

Nguyen, Duc; Goings, Joshua J.; Nguyen, Huy A.; Lyding, Joseph; Li, Xiaosong; Gruebele, Martin

2018-02-01

We previously demonstrated that we can image electronic excitations of quantum dots by single-molecule absorption scanning tunneling microscopy (SMA-STM). With this technique, a modulated laser beam periodically saturates an electronic transition of a single nanoparticle, and the resulting tunneling current modulation ΔI(x0, y0) maps out the SMA-STM image. In this paper, we first derive the basic theory to calculate ΔI(x0, y0) in the one-electron approximation. For near-resonant tunneling through an empty orbital "i" of the nanostructure, the SMA-STM signal is approximately proportional to the electron density |φi) (x0,y0)|

Orientation-dependent imaging of electronically excited quantum dots.

PubMed

Nguyen, Duc; Goings, Joshua J; Nguyen, Huy A; Lyding, Joseph; Li, Xiaosong; Gruebele, Martin

2018-02-14

We previously demonstrated that we can image electronic excitations of quantum dots by single-molecule absorption scanning tunneling microscopy (SMA-STM). With this technique, a modulated laser beam periodically saturates an electronic transition of a single nanoparticle, and the resulting tunneling current modulation ΔI(x 0 , y 0 ) maps out the SMA-STM image. In this paper, we first derive the basic theory to calculate ΔI(x 0 , y 0 ) in the one-electron approximation. For near-resonant tunneling through an empty orbital "i" of the nanostructure, the SMA-STM signal is approximately proportional to the electron density φ i x 0 ,y 0 2 of the excited orbital in the tunneling region. Thus, the SMA-STM signal is approximated by an orbital density map (ODM) of the resonantly excited orbital at energy E i . The situation is more complex for correlated electron motion, but either way a slice through the excited electronic state structure in the tunneling region is imaged. We then show experimentally that we can nudge quantum dots on the surface and roll them, thus imaging excited state electronic structure of a single quantum dot at different orientations. We use density functional theory to model ODMs at various orientations, for qualitative comparison with the SMA-STM experiment. The model demonstrates that our experimentally observed signal monitors excited states, localized by defects near the surface of an individual quantum dot. The sub-nanometer super-resolution imaging technique demonstrated here could become useful for mapping out the three-dimensional structure of excited states localized by defects within nanomaterials.

Chirp- and random-based coded ultrasonic excitation for localized blood-brain barrier opening

PubMed Central

Kamimura, HAS; Wang, S; Wu, S-Y; Karakatsani, ME; Acosta, C; Carneiro, AAO; Konofagou, EE

2015-01-01

Chirp- and random-based coded excitation methods have been proposed to reduce standing wave formation and improve focusing of transcranial ultrasound. However, no clear evidence has been shown to support the benefits of these ultrasonic excitation sequences in vivo. This study evaluates the chirp and periodic selection of random frequency (PSRF) coded-excitation methods for opening the blood-brain barrier (BBB) in mice. Three groups of mice (n=15) were injected with polydisperse microbubbles and sonicated in the caudate putamen using the chirp/PSRF coded (bandwidth: 1.5-1.9 MHz, peak negative pressure: 0.52 MPa, duration: 30 s) or standard ultrasound (frequency: 1.5 MHz, pressure: 0.52 MPa, burst duration: 20 ms, duration: 5 min) sequences. T1-weighted contrast-enhanced MRI scans were performed to quantitatively analyze focused ultrasound induced BBB opening. The mean opening volumes evaluated from the MRI were 9.38±5.71 mm3, 8.91±3.91 mm3 and 35.47 ± 5.10 mm3 for the chirp, random and regular sonications, respectively. The mean cavitation levels were 55.40±28.43 V.s, 63.87±29.97 V.s and 356.52±257.15 V.s for the chirp, random and regular sonications, respectively. The chirp and PSRF coded pulsing sequences improved the BBB opening localization by inducing lower cavitation levels and smaller opening volumes compared to results of the regular sonication technique. Larger bandwidths were associated with more focused targeting but were limited by the frequency response of the transducer, the skull attenuation and the microbubbles optimal frequency range. The coded methods could therefore facilitate highly localized drug delivery as well as benefit other transcranial ultrasound techniques that use higher pressure levels and higher precision to induce the necessary bioeffects in a brain region while avoiding damage to the surrounding healthy tissue. PMID:26394091

Intravital Fluorescence Excitation in Whole-Animal Optical Imaging.

PubMed

Nooshabadi, Fatemeh; Yang, Hee-Jeong; Bixler, Joel N; Kong, Ying; Cirillo, Jeffrey D; Maitland, Kristen C

2016-01-01

Whole-animal fluorescence imaging with recombinant or fluorescently-tagged pathogens or cells enables real-time analysis of disease progression and treatment response in live animals. Tissue absorption limits penetration of fluorescence excitation light, particularly in the visible wavelength range, resulting in reduced sensitivity to deep targets. Here, we demonstrate the use of an optical fiber bundle to deliver light into the mouse lung to excite fluorescent bacteria, circumventing tissue absorption of excitation light in whole-animal imaging. We present the use of this technology to improve detection of recombinant reporter strains of tdTomato-expressing Mycobacterium bovis BCG (Bacillus Calmette Guerin) bacteria in the mouse lung. A microendoscope was integrated into a whole-animal fluorescence imager to enable intravital excitation in the mouse lung with whole-animal detection. Using this technique, the threshold of detection was measured as 103 colony forming units (CFU) during pulmonary infection. In comparison, the threshold of detection for whole-animal fluorescence imaging using standard epi-illumination was greater than 106 CFU.

Intravital Fluorescence Excitation in Whole-Animal Optical Imaging

PubMed Central

Bixler, Joel N.; Kong, Ying; Cirillo, Jeffrey D.; Maitland, Kristen C.

2016-01-01

Whole-animal fluorescence imaging with recombinant or fluorescently-tagged pathogens or cells enables real-time analysis of disease progression and treatment response in live animals. Tissue absorption limits penetration of fluorescence excitation light, particularly in the visible wavelength range, resulting in reduced sensitivity to deep targets. Here, we demonstrate the use of an optical fiber bundle to deliver light into the mouse lung to excite fluorescent bacteria, circumventing tissue absorption of excitation light in whole-animal imaging. We present the use of this technology to improve detection of recombinant reporter strains of tdTomato-expressing Mycobacterium bovis BCG (Bacillus Calmette Guerin) bacteria in the mouse lung. A microendoscope was integrated into a whole-animal fluorescence imager to enable intravital excitation in the mouse lung with whole-animal detection. Using this technique, the threshold of detection was measured as 103 colony forming units (CFU) during pulmonary infection. In comparison, the threshold of detection for whole-animal fluorescence imaging using standard epi-illumination was greater than 106 CFU. PMID:26901051

Analysis of Rapid Multi-Focal Zone ARFI Imaging

PubMed Central

Rosenzweig, Stephen; Palmeri, Mark; Nightingale, Kathryn

2015-01-01

Acoustic radiation force impulse (ARFI) imaging has shown promise for visualizing structure and pathology within multiple organs; however, because the contrast depends on the push beam excitation width, image quality suffers outside of the region of excitation. Multi-focal zone ARFI imaging has previously been used to extend the region of excitation (ROE), but the increased acquisition duration and acoustic exposure have limited its utility. Supersonic shear wave imaging has previously demonstrated that through technological improvements in ultrasound scanners and power supplies, it is possible to rapidly push at multiple locations prior to tracking displacements, facilitating extended depth of field shear wave sources. Similarly, ARFI imaging can utilize these same radiation force excitations to achieve tight pushing beams with a large depth of field. Finite element method simulations and experimental data are presented demonstrating that single- and rapid multi-focal zone ARFI have comparable image quality (less than 20% loss in contrast), but the multi-focal zone approach has an extended axial region of excitation. Additionally, as compared to single push sequences, the rapid multi-focal zone acquisitions improve the contrast to noise ratio by up to 40% in an example 4 mm diameter lesion. PMID:25643078

Energy-Looping Nanoparticles: Harnessing Excited-State Absorption for Deep-Tissue Imaging.

PubMed

Levy, Elizabeth S; Tajon, Cheryl A; Bischof, Thomas S; Iafrati, Jillian; Fernandez-Bravo, Angel; Garfield, David J; Chamanzar, Maysamreza; Maharbiz, Michel M; Sohal, Vikaas S; Schuck, P James; Cohen, Bruce E; Chan, Emory M

2016-09-27

Near infrared (NIR) microscopy enables noninvasive imaging in tissue, particularly in the NIR-II spectral range (1000-1400 nm) where attenuation due to tissue scattering and absorption is minimized. Lanthanide-doped upconverting nanocrystals are promising deep-tissue imaging probes due to their photostable emission in the visible and NIR, but these materials are not efficiently excited at NIR-II wavelengths due to the dearth of lanthanide ground-state absorption transitions in this window. Here, we develop a class of lanthanide-doped imaging probes that harness an energy-looping mechanism that facilitates excitation at NIR-II wavelengths, such as 1064 nm, that are resonant with excited-state absorption transitions but not ground-state absorption. Using computational methods and combinatorial screening, we have identified Tm(3+)-doped NaYF4 nanoparticles as efficient looping systems that emit at 800 nm under continuous-wave excitation at 1064 nm. Using this benign excitation with standard confocal microscopy, energy-looping nanoparticles (ELNPs) are imaged in cultured mammalian cells and through brain tissue without autofluorescence. The 1 mm imaging depths and 2 μm feature sizes are comparable to those demonstrated by state-of-the-art multiphoton techniques, illustrating that ELNPs are a promising class of NIR probes for high-fidelity visualization in cells and tissue.

Hyperspectral imaging fluorescence excitation scanning for detecting colorectal cancer: pilot study

NASA Astrophysics Data System (ADS)

Leavesley, Silas J.; Wheeler, Mikayla; Lopez, Carmen; Baker, Thomas; Favreau, Peter F.; Rich, Thomas C.; Rider, Paul F.; Boudreaux, Carole W.

2016-03-01

Optical spectroscopy and hyperspectral imaging have shown the theoretical potential to discriminate between cancerous and non-cancerous tissue with high sensitivity and specificity. To date, these techniques have not been able to be effectively translated to endoscope platforms. Hyperspectral imaging of the fluorescence excitation spectrum represents a new technology that may be well-suited for endoscopic implementation. However, the feasibility of detecting differences between normal and cancerous mucosa using fluorescence excitation-scanning hyperspectral imaging has not been evaluated. The objective of this pilot study was to evaluate the changes in the fluorescence excitation spectrum of resected specimen pairs of colorectal adenocarcinoma and normal colorectal mucosa. Patients being treated for colorectal adenocarcinoma were enrolled. Representative adenocarcinoma and normal colonic mucosa specimens were collected from each case. Specimens were flash frozen in liquid nitrogen. Adenocarcinoma was confirmed by histologic evaluation of H&E permanent sections. Hyperspectral image data of the fluorescence excitation of adenocarcinoma and surrounding normal tissue were acquired using a custom microscope configuration previously developed in our lab. Results demonstrated consistent spectral differences between normal and cancerous tissues over the fluorescence excitation spectral range of 390-450 nm. We conclude that fluorescence excitation-scanning hyperspectral imaging may offer an alternative approach for differentiating adenocarcinoma and surrounding normal mucosa of the colon. Future work will focus on expanding the number of specimen pairs analyzed and will utilize fresh tissues where possible, as flash freezing and reconstituting tissues may have altered the autofluorescence properties.

Microwave-excited ultrasound and thermoacoustic dual imaging

NASA Astrophysics Data System (ADS)

Ding, Wenzheng; Ji, Zhong; Xing, Da

2017-05-01

We designed a microwave-excited ultrasound (MUI) and thermoacoustic dual imaging system. Under the pulsed microwave excitation, the piezoelectric transducer used for thermoacoustic signal detection will also emit a highly directional ultrasonic beam based on the inverse piezoelectric effect. With this beam, the ultrasonic transmitter circuitry of the traditional ultrasound imaging (TUI) system can be replaced by a microwave source. In other words, TUI can be fully integrated into the thermoacoustic imaging system by sharing the microwave excitation source and the transducer. Moreover, the signals of the two imaging modalities do not interfere with each other due to the existence of the sound path difference, so that MUI can be performed simultaneously with microwave-induced thermoacoustic imaging. In the study, the performance characteristics and imaging capabilities of this hybrid system are demonstrated. The results indicate that our design provides one easy method for low-cost platform integration and has the potential to offer a clinically useful dual-modality tool for the detection of accurate diseases.

Improving confocal microscopy with solid-state semiconductor excitation sources

NASA Astrophysics Data System (ADS)

Sivers, Nelson L.

To efficiently excite the fluorescent dyes used in imaging biological samples with a confocal microscope, the wavelengths of the exciting laser must be near the fluorochrome absorption peak. However, this causes imaging problems when the fluorochrome absorption and emission spectra overlap significantly, i.e. have small Stokes shifts, which is the case for most fluorochromes that emit in the red to infrared. As a result, the reflected laser excitation cannot be distinguished from the information-containing fluorescence signal. However, cryogenically cooling the exciting laser diode enabled the laser emission wavelengths to be tuned to shorter wavelengths, decreasing the interference between the laser and the fluorochrome's fluorescence. This reduced the amount of reflected laser light in the confocal image. However, the cooled laser diode's shorter wavelength signal resulted in slightly less efficient fluorochrome excitation. Spectrophotometric analysis showed that as the laser diodes were cooled, their output power increased, which more than compensated for the lower fluorochrome excitation and resulted in significantly more intense fluorescence. Thus, by tuning the laser diode emission wavelengths away from the fluorescence signal, less reflected laser light and more fluorescence information reached the detector, creating images with better signal to noise ratios. Additionally, new, high, luminous flux, light-emitting diodes (LEDs) are now powerful enough to create confocal fluorescence signals comparable to those produced by the traditional laser excitation sources in fluorescence confocal microscopes. The broader LED spectral response effectively excited the fluorochrome, yet was spectrally limited enough for standard filter sets to separate the LED excitation from the fluorochrome fluorescence signal. Spectrophotometric analysis of the excitation and fluorescence spectra of several fluorochromes showed that high-powered, LED-induced fluorescence contained the same spectral information and could be more intense than that produced by lasers. An alternative, LED-based, confocal microscope is proposed in this thesis that would be capable of exciting multiple fluorochromes in a single specimen, producing images of several distinct cellular components simultaneously. The inexpensive, LED-based, confocal microscope would require lower peak excitation intensities to produce fluorescence signals equal to those produced by laser excitation, reducing cellular damage and slowing fluorochrome photobleaching.

A pulse coding and decoding strategy to perform Lamb wave inspections using simultaneously multiple actuators

NASA Astrophysics Data System (ADS)

De Marchi, Luca; Marzani, Alessandro; Moll, Jochen; Kudela, Paweł; Radzieński, Maciej; Ostachowicz, Wiesław

2017-07-01

The performance of Lamb wave based monitoring systems, both in terms of diagnosis time and data complexity, can be enhanced by increasing the number of transducers used to actuate simultaneously the guided waves in the inspected medium. However, in case of multiple simultaneously-operated actuators the interference among the excited wave modes within the acquired signals has to be considered for the further processing. To this aim, in this work a code division strategy based on the Warped Frequency Transform is presented. At first, the proposed procedure encodes actuation pulses using Gold sequences. Next, for each considered actuator the acquired signals are compensated from dispersion by cross correlating the warped version of the actuated and received signals. Compensated signals form the base for a final wavenumber imaging meant at emphasizing defects and or anomalies by removing incident wavefield and edge reflections. The proposed strategy is tested numerically, and validated through an experiment in which guided waves are actuated in a plate by four piezoelectric transducers operating simultaneously.

Determining mode excitations of vacuum electronics devices via three-dimensional simulations using the SOS code

NASA Technical Reports Server (NTRS)

Warren, Gary

1988-01-01

The SOS code is used to compute the resonance modes (frequency-domain information) of sample devices and separately to compute the transient behavior of the same devices. A code, DOT, is created to compute appropriate dot products of the time-domain and frequency-domain results. The transient behavior of individual modes in the device is then plotted. Modes in a coupled-cavity traveling-wave tube (CCTWT) section excited beam in separate simulations are analyzed. Mode energy vs. time and mode phase vs. time are computed and it is determined whether the transient waves are forward or backward waves for each case. Finally, the hot-test mode frequencies of the CCTWT section are computed.

Spatial transform coding of color images.

NASA Technical Reports Server (NTRS)

Pratt, W. K.

1971-01-01

The application of the transform-coding concept to the coding of color images represented by three primary color planes of data is discussed. The principles of spatial transform coding are reviewed and the merits of various methods of color-image representation are examined. A performance analysis is presented for the color-image transform-coding system. Results of a computer simulation of the coding system are also given. It is shown that, by transform coding, the chrominance content of a color image can be coded with an average of 1.0 bits per element or less without serious degradation. If luminance coding is also employed, the average rate reduces to about 2.0 bits per element or less.

Coded excitation speeds up the detection of the fundamental flexural guided wave in coated tubes

NASA Astrophysics Data System (ADS)

Song, Xiaojun; Moilanen, Petro; Zhao, Zuomin; Ta, Dean; Pirhonen, Jalmari; Salmi, Ari; Hæeggström, Edward; Myllylä, Risto; Timonen, Jussi; Wang, Weiqi

2016-09-01

The fundamental flexural guided wave (FFGW) permits ultrasonic assessment of the wall thickness of solid waveguides, such as tubes or, e.g., long cortical bones. Recently, an optical non-contact method was proposed for ultrasound excitation and detection with the aim of facilitating the FFGW reception by suppressing the interfering modes from the soft coating. This technique suffers from low SNR and requires iterative physical scanning across the source-receiver distance for 2D-FFT analysis. This means that SNR improvement achieved by temporal averaging becomes time-consuming (several minutes) which reduces the applicability of the technique, especially in time-critical applications such as clinical quantitative ultrasound. To achieve sufficient SNR faster, an ultrasonic excitation by a base-sequence-modulated Golay code (BSGC, 64-bit code pair) on coated tube samples (1-5 mm wall thickness and 5 mm soft coating layer) was used. This approach improved SNR by 21 dB and speeded up the measurement by a factor of 100 compared to using a classical pulse excitation with temporal averaging. The measurement now took seconds instead of minutes, while the ability to determine the wall thickness of the phantoms was maintained. The technique thus allows rapid noncontacting assessment of the wall thickness in coated solid tubes, such as the human bone.

Information coding with frequency of oscillations in Belousov-Zhabotinsky encapsulated disks

NASA Astrophysics Data System (ADS)

Gorecki, J.; Gorecka, J. N.; Adamatzky, Andrew

2014-04-01

Information processing with an excitable chemical medium, like the Belousov-Zhabotinsky (BZ) reaction, is typically based on information coding in the presence or absence of excitation pulses. Here we present a new concept of Boolean coding that can be applied to an oscillatory medium. A medium represents the logical TRUE state if a selected region oscillates with a high frequency. If the frequency fails below a specified value, it represents the logical FALSE state. We consider a medium composed of disks encapsulating an oscillatory mixture of reagents, as related to our recent experiments with lipid-coated BZ droplets. We demonstrate that by using specific geometrical arrangements of disks containing the oscillatory medium one can perform logical operations on variables coded in oscillation frequency. Realizations of a chemical signal diode and of a single-bit memory with oscillatory disks are also discussed.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gupta, Bipin Kumar, E-mail: bipinbhu@yahoo.com; Kumar, Arun; Amity Institute of Applied Science, Amity University, Noida, Uttar Pradesh 201303

Herein, a novel green emitting long-persistent Sr{sub 3}SiAl{sub 4}O{sub 11}:Eu{sup 2+}/Dy{sup 3+} phosphor was synthesized in a single phase form using facile solid state reaction method under the reducing atmosphere of 10% H{sub 2} and 90% N{sub 2}. The resulting phosphor exhibits hyper-sensitive strong broad green emission, peaking at 510 nm upon 340 nm excitation wavelength, which is attributed to the 4f{sup 6}5d{sup 1}-4f{sup 7} transitions of emission center of europium (Eu{sup 2+}) ions. Moreover, the incorporation of dysprosium (Dy{sup 3+}) ions, which act as effective hole trap centers with appropriate depth, largely enhances the photoluminescence characteristics and greatly improves the persistentmore » intense luminescence behavior of Sr{sub 3}SiAl{sub 4}O{sub 11}:Eu{sup 2+}/Dy{sup 3+} phosphor under ultraviolet (UV) excitation. In addition, with the optimum doping concentration and sufficient UV excitation time period, the as-synthesized phosphor can be persisted afterglow for time duration ∼4 h with maximum luminescence intensity. Thus, these results suggest that this phosphor could be expected as an ultimate choice for next generation advanced luminescent materials in security applications such as latent finger-marks detection, photo-masking induced phosphorescent images, and security code detection.« less

Mutations in GPR143/OA1 and ABCA4 Inform Interpretations of Short-Wavelength and Near-Infrared Fundus Autofluorescence

PubMed Central

Paavo, Maarjaliis; Zhao, Jin; Kim, Hye Jin; Lee, Winston; Zernant, Jana; Cai, Carolyn; Allikmets, Rando; Tsang, Stephen H.; Sparrow, Janet R.

2018-01-01

Purpose We sought to advance interpretations and quantification of short-wavelength fundus autofluorescence (SW-AF) emitted from bisretinoid lipofuscin and near-infrared autofluoresence (NIR-AF) originating from melanin. Methods Carriers of mutations in X-linked GPR143/OA1, a common form of ocular albinism; patients with confirmed mutations in ABCA4 conferring increased SW-AF; and subjects with healthy eyes were studied. SW-AF (488 nm excitation, 500–680 nm emission) and NIR-AF (excitation 787 nm, emission >830 nm) images were acquired with a confocal scanning laser ophthalmoscope. SW-AF images were analyzed for quantitative autofluoresence (qAF). Analogous methods of image acquisition and analysis were performed in albino and pigmented Abca4−/− mice and wild-type mice. Results Quantitation of SW-AF (qAF), construction of qAF color-coded maps, and examination of NIR-AF images from GPR143/OA1 carriers revealed mosaics in which patches of fundus exhibiting NIR-AF signal had qAF levels within normal limits whereas the hypopigmented areas in the NIR-AF image corresponded to foci of elevated qAF. qAF also was increased in albino versus pigmented mice. Although melanin contributes to fundus infrared reflectance, the latter appeared to be uniform in en face reflectance images of GPR143/OA1-carriers. In patients diagnosed with ABCA4-associated disease, NIR-AF increased in tandem with increased qAF originating in bisretinoid lipofuscin. Similarly in Abca4−/− mice having increased SW-AF, NIR-AF was more pronounced than in wild-type mice. Conclusions These studies corroborate RPE melanin as the major source of NIR-AF but also indicate that bisretinoid lipofuscin, when present at sufficient concentrations, contributes to the NIR-AF signal. Ocular melanin attenuates the SW-AF signal.

Three-Photon Luminescence of Gold Nanorods and Its Applications for High Contrast Tissue and Deep In Vivo Brain Imaging

PubMed Central

Wang, Shaowei; Xi, Wang; Cai, Fuhong; Zhao, Xinyuan; Xu, Zhengping; Qian, Jun; He, Sailing

2015-01-01

Gold nanoparticles can be used as contrast agents for bio-imaging applications. Here we studied multi-photon luminescence (MPL) of gold nanorods (GNRs), under the excitation of femtosecond (fs) lasers. GNRs functionalized with polyethylene glycol (PEG) molecules have high chemical and optical stability, and can be used as multi-photon luminescent nanoprobes for deep in vivo imaging of live animals. We have found that the depth of in vivo imaging is dependent upon the transmission and focal capability of the excitation light interacting with the GNRs. Our study focused on the comparison of MPL from GNRs with two different aspect ratios, as well as their ex vivo and in vivo imaging effects under 760 nm and 1000 nm excitation, respectively. Both of these wavelengths were located at an optically transparent window of biological tissue (700-1000 nm). PEGylated GNRs, which were intravenously injected into mice via the tail vein and accumulated in major organs and tumor tissue, showed high image contrast due to distinct three-photon luminescence (3PL) signals upon irradiation of a 1000 nm fs laser. Concerning in vivo mouse brain imaging, the 3PL imaging depth of GNRs under 1000 nm fs excitation could reach 600 μm, which was approximately 170 μm deeper than the two-photon luminescence (2PL) imaging depth of GNRs with a fs excitation of 760 nm. PMID:25553113

Radiation pressure excitation of a low temperature atomic force/magnetic force microscope for imaging in 4-300 K temperature range

NASA Astrophysics Data System (ADS)

Ćelik, Ümit; Karcı, Özgür; Uysallı, Yiǧit; Özer, H. Özgür; Oral, Ahmet

2017-01-01

We describe a novel radiation pressure based cantilever excitation method for imaging in dynamic mode atomic force microscopy (AFM) for the first time. Piezo-excitation is the most common method for cantilever excitation, however it may cause spurious resonance peaks. Therefore, the direct excitation of the cantilever plays a crucial role in AFM imaging. A fiber optic interferometer with a 1310 nm laser was used both for the excitation of the cantilever at the resonance and the deflection measurement of the cantilever in a commercial low temperature atomic force microscope/magnetic force microscope (AFM/MFM) from NanoMagnetics Instruments. The laser power was modulated at the cantilever's resonance frequency by a digital Phase Locked Loop (PLL). The laser beam is typically modulated by ˜500 μW, and ˜141.8 nmpp oscillation amplitude is obtained in moderate vacuum levels between 4 and 300 K. We have demonstrated the performance of the radiation pressure excitation in AFM/MFM by imaging atomic steps in graphite, magnetic domains in CoPt multilayers between 4 and 300 K and Abrikosov vortex lattice in BSCCO(2212) single crystal at 4 K for the first time.

Radiation pressure excitation of a low temperature atomic force/magnetic force microscope for imaging in 4-300 K temperature range.

PubMed

Çelik, Ümit; Karcı, Özgür; Uysallı, Yiğit; Özer, H Özgür; Oral, Ahmet

2017-01-01

We describe a novel radiation pressure based cantilever excitation method for imaging in dynamic mode atomic force microscopy (AFM) for the first time. Piezo-excitation is the most common method for cantilever excitation, however it may cause spurious resonance peaks. Therefore, the direct excitation of the cantilever plays a crucial role in AFM imaging. A fiber optic interferometer with a 1310 nm laser was used both for the excitation of the cantilever at the resonance and the deflection measurement of the cantilever in a commercial low temperature atomic force microscope/magnetic force microscope (AFM/MFM) from NanoMagnetics Instruments. The laser power was modulated at the cantilever's resonance frequency by a digital Phase Locked Loop (PLL). The laser beam is typically modulated by ∼500 μW, and ∼141.8 nm pp oscillation amplitude is obtained in moderate vacuum levels between 4 and 300 K. We have demonstrated the performance of the radiation pressure excitation in AFM/MFM by imaging atomic steps in graphite, magnetic domains in CoPt multilayers between 4 and 300 K and Abrikosov vortex lattice in BSCCO(2212) single crystal at 4 K for the first time.

Reconstructing photorealistic 3D models from image sequence using domain decomposition method

NASA Astrophysics Data System (ADS)

Xiong, Hanwei; Pan, Ming; Zhang, Xiangwei

2009-11-01

In the fields of industrial design, artistic design and heritage conservation, physical objects are usually digitalized by reverse engineering through some 3D scanning methods. Structured light and photogrammetry are two main methods to acquire 3D information, and both are expensive. Even if these expensive instruments are used, photorealistic 3D models are seldom available. In this paper, a new method to reconstruction photorealistic 3D models using a single camera is proposed. A square plate glued with coded marks is used to place the objects, and a sequence of about 20 images is taken. From the coded marks, the images are calibrated, and a snake algorithm is used to segment object from the background. A rough 3d model is obtained using shape from silhouettes algorithm. The silhouettes are decomposed into a combination of convex curves, which are used to partition the rough 3d model into some convex mesh patches. For each patch, the multi-view photo consistency constraints and smooth regulations are expressed as a finite element formulation, which can be resolved locally, and the information can be exchanged along the patches boundaries. The rough model is deformed into a fine 3d model through such a domain decomposition finite element method. The textures are assigned to each element mesh, and a photorealistic 3D model is got finally. A toy pig is used to verify the algorithm, and the result is exciting.

Colorectal cancer detection by hyperspectral imaging using fluorescence excitation scanning

NASA Astrophysics Data System (ADS)

Leavesley, Silas J.; Deal, Joshua; Hill, Shante; Martin, Will A.; Lall, Malvika; Lopez, Carmen; Rider, Paul F.; Rich, Thomas C.; Boudreaux, Carole W.

2018-02-01

Hyperspectral imaging technologies have shown great promise for biomedical applications. These techniques have been especially useful for detection of molecular events and characterization of cell, tissue, and biomaterial composition. Unfortunately, hyperspectral imaging technologies have been slow to translate to clinical devices - likely due to increased cost and complexity of the technology as well as long acquisition times often required to sample a spectral image. We have demonstrated that hyperspectral imaging approaches which scan the fluorescence excitation spectrum can provide increased signal strength and faster imaging, compared to traditional emission-scanning approaches. We have also demonstrated that excitation-scanning approaches may be able to detect spectral differences between colonic adenomas and adenocarcinomas and normal mucosa in flash-frozen tissues. Here, we report feasibility results from using excitation-scanning hyperspectral imaging to screen pairs of fresh tumoral and nontumoral colorectal tissues. Tissues were imaged using a novel hyperspectral imaging fluorescence excitation scanning microscope, sampling a wavelength range of 360-550 nm, at 5 nm increments. Image data were corrected to achieve a NIST-traceable flat spectral response. Image data were then analyzed using a range of supervised and unsupervised classification approaches within ENVI software (Harris Geospatial Solutions). Supervised classification resulted in >99% accuracy for single-patient image data, but only 64% accuracy for multi-patient classification (n=9 to date), with the drop in accuracy due to increased false-positive detection rates. Hence, initial data indicate that this approach may be a viable detection approach, but that larger patient sample sizes need to be evaluated and the effects of inter-patient variability studied.

Exploring infrared neural stimulation with multimodal nonlinear imaging (Conference Presentation)

NASA Astrophysics Data System (ADS)

Adams, Wilson R.; Mahadevan-Jansen, Anita

2017-02-01

Infrared neural stimulation (INS) provides optical control of neural excitability using near to mid-infrared (mid-IR) light, which allows for spatially selective, artifact-free excitation without the introduction of exogenous agents or genetic modification. Although neural excitability is mediated by a transient temperature increase due to water absorption of IR energy, the molecular nature of IR excitability in neural tissue remains unknown. Current research suggests that transient changes in local tissue temperature give rise to a myriad of cellular responses that have been individually attributed to IR mediated excitability. To further elucidate the underlying biophysical mechanisms, we have begun work towards employing a novel multimodal nonlinear imaging platform to probe the molecular underpinnings of INS. Our imaging system performs coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS), two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG) and thermal imaging into a single platform that allows for unprecedented co-registration of thermal and biochemical information in real-time. Here, we present our work leveraging CARS and SRS in acute thalamocortical brain slice preparations. We observe the evolution of lipid and protein-specific Raman bands during INS and electrically evoked activity in real-time. Combined with two-photon fluorescence and second harmonic generation, we offer insight to cellular metabolism and membrane dynamics during INS. Thermal imaging allows for the coregistration of acquired biochemical information with temperature information. Our work previews the versatility and capabilities of coherent Raman imaging combined with multiphoton imaging to observe biophysical phenomena for neuroscience applications.

Survey Of Lossless Image Coding Techniques

NASA Astrophysics Data System (ADS)

Melnychuck, Paul W.; Rabbani, Majid

1989-04-01

Many image transmission/storage applications requiring some form of data compression additionally require that the decoded image be an exact replica of the original. Lossless image coding algorithms meet this requirement by generating a decoded image that is numerically identical to the original. Several lossless coding techniques are modifications of well-known lossy schemes, whereas others are new. Traditional Markov-based models and newer arithmetic coding techniques are applied to predictive coding, bit plane processing, and lossy plus residual coding. Generally speaking, the compression ratio offered by these techniques are in the area of 1.6:1 to 3:1 for 8-bit pictorial images. Compression ratios for 12-bit radiological images approach 3:1, as these images have less detailed structure, and hence, their higher pel correlation leads to a greater removal of image redundancy.

20 MHz Forward-imaging Single-element Beam Steering with an Internal Rotating Variable-Angle Reflecting Surface: Wire phantom and Ex vivo pilot study

PubMed Central

Raphael, David T.; Li, Xiang; Park, Jinhyoung; Chen, Ruimin; Chabok, Hamid; Barukh, Arthur; Zhou, Qifa; Elgazery, Mahmoud; Shung, K. Kirk

2012-01-01

Feasibility is demonstrated for a forward-imaging beam steering system involving a single-element 20 MHz angled-face acoustic transducer combined with an internal rotating variable-angle reflecting surface (VARS). Rotation of the VARS structure, for a fixed position of the transducer, generates a 2-D angular sector scan. If these VARS revolutions were to be accompanied by successive rotations of the single-element transducer, 3-D imaging would be achieved. In the design of this device, a single-element 20 MHz PMN-PT press-focused angled-face transducer is focused on the circle of midpoints of a micro-machined VARS within the distal end of an endoscope. The 2-D imaging system was tested in water bath experiments with phantom wire structures at a depth of 10 mm, and exhibited an axial resolution of 66 μm and a lateral resolution of 520 μm. Chirp coded excitation was used to enhance the signal-to-noise ratio, and to increase the depth of penetration. Images of an ex vivo cow eye were obtained. This VARS-based approach offers a novel forward-looking beam-steering method, which could be useful in intra-cavity imaging. PMID:23122968

20 MHz forward-imaging single-element beam steering with an internal rotating variable-angle reflecting surface: Wire phantom and ex vivo pilot study.

PubMed

Raphael, David T; Li, Xiang; Park, Jinhyoung; Chen, Ruimin; Chabok, Hamid; Barukh, Arthur; Zhou, Qifa; Elgazery, Mahmoud; Shung, K Kirk

2013-02-01

Feasibility is demonstrated for a forward-imaging beam steering system involving a single-element 20MHz angled-face acoustic transducer combined with an internal rotating variable-angle reflecting surface (VARS). Rotation of the VARS structure, for a fixed position of the transducer, generates a 2-D angular sector scan. If these VARS revolutions were to be accompanied by successive rotations of the single-element transducer, 3-D imaging would be achieved. In the design of this device, a single-element 20MHz PMN-PT press-focused angled-face transducer is focused on the circle of midpoints of a micro-machined VARS within the distal end of an endoscope. The 2-D imaging system was tested in water bath experiments with phantom wire structures at a depth of 10mm, and exhibited an axial resolution of 66μm and a lateral resolution of 520μm. Chirp coded excitation was used to enhance the signal-to-noise ratio, and to increase the depth of penetration. Images of an ex vivo cow eye were obtained. This VARS-based approach offers a novel forward-looking beam-steering method, which could be useful in intra-cavity imaging. Copyright © 2012 Elsevier B.V. All rights reserved.

Advanced synchronous luminescence imaging for chemical and medical diagnostics

DOEpatents

Vo-Dinh, Tuan

2006-09-05

A diagnostic method and associated system includes the steps of exposing at least one sample location with excitation radiation through a single optical waveguide or a single optical waveguide bundle, wherein the sample emits emission radiation in response to the excitation radiation. The same single optical waveguide or the single optical waveguide bundle receives at least a portion of the emission radiation from the sample, thus providing co-registration of the excitation radiation and the emission radiation. The wavelength of the excitation radiation and emission radiation is synchronously scanned to produce a spectrum upon which an image can be formed. An increased emission signal is generated by the enhanced overlap of the excitation and emission focal volumes provided by co-registration of the excitation and emission signals thus increasing the sensitivity as well as decreasing the exposure time necessary to obtain an image.

Three-dimensional multiexcitation magnetoacoustic tomography with magnetic induction

PubMed Central

Li, Xu; Mariappan, Leo; He, Bin

2010-01-01

Magnetoacoustic tomography with magnetic induction (MAT-MI) is a hybrid imaging modality proposed to image electrical conductivity contrast of biological tissue with high spatial resolution. This modality combines magnetic excitations with ultrasound detection through the Lorentz force based coupling mechanism. However, previous studies have shown that MAT-MI method with single type of magnetic excitation can only reconstruct the conductivity boundaries of a sample. In order to achieve more complete conductivity contrast reconstruction, we proposed a multiexcitation MAT-MI approach. In this approach, multiple magnetic excitations using different coil configurations are applied to the object sequentially and ultrasonic signals corresponding to each excitation are collected for conductivity image reconstruction. In this study, we validate the new multiexcitation MAT-MI method for three-dimensional (3D) conductivity imaging through both computer simulations and phantom experiments. 3D volume data are obtained by utilizing acoustic focusing and cylindrical scanning under each magnetic excitation. It is shown in our simulation and experiment results that with a common ultrasound probe that has limited bandwidth we are able to correctly reconstruct the 3D relative conductivity contrast of the imaging object. As compared to those conductivity boundary images generated by previous single-excitation MAT-MI, the new multiexcitation MAT-MI method provides more complete conductivity contrast reconstruction, and therefore, more valuable information in possible clinical and research applications. PMID:21267084

Recent Livermore Excitation and Dielectronic Recombination Measurements for Laboratory and Astrophysical Spectral Modeling

NASA Technical Reports Server (NTRS)

Beiersdorfer, P.; Brown, G. V.; Gu, M.-F.; Harris, C. L.; Kahn, S. M.; Kim, S.-H.; Neill, P. A.; Savin, D. W.; Smith, A. J.; Utter, S. B.

2000-01-01

Using the EBIT facility in Livermore we produce definitive atomic data for input into spectral synthesis codes. Recent measurements of line excitation and dielectronic recombination of highly charged K-shell and L-shell ions are presented to illustrate this point.

Quantum nonlinear optics without photons

NASA Astrophysics Data System (ADS)

Stassi, Roberto; Macrı, Vincenzo; Kockum, Anton Frisk; Di Stefano, Omar; Miranowicz, Adam; Savasta, Salvatore; Nori, Franco

2017-08-01

Spontaneous parametric down-conversion is a well-known process in quantum nonlinear optics in which a photon incident on a nonlinear crystal spontaneously splits into two photons. Here we propose an analogous physical process where one excited atom directly transfers its excitation to a pair of spatially separated atoms with probability approaching 1. The interaction is mediated by the exchange of virtual rather than real photons. This nonlinear atomic process is coherent and reversible, so the pair of excited atoms can transfer the excitation back to the first one: the atomic analog of sum-frequency generation of light. The parameters used to investigate this process correspond to experimentally demonstrated values in ultrastrong circuit quantum electrodynamics. This approach can be extended to realize other nonlinear interatomic processes, such as four-atom mixing, and is an attractive architecture for the realization of quantum devices on a chip. We show that four-qubit mixing can efficiently implement quantum repetition codes and, thus, can be used for error-correction codes.

Research on pre-processing of QR Code

NASA Astrophysics Data System (ADS)

Sun, Haixing; Xia, Haojie; Dong, Ning

2013-10-01

QR code encodes many kinds of information because of its advantages: large storage capacity, high reliability, full arrange of utter-high-speed reading, small printing size and high-efficient representation of Chinese characters, etc. In order to obtain the clearer binarization image from complex background, and improve the recognition rate of QR code, this paper researches on pre-processing methods of QR code (Quick Response Code), and shows algorithms and results of image pre-processing for QR code recognition. Improve the conventional method by changing the Souvola's adaptive text recognition method. Additionally, introduce the QR code Extraction which adapts to different image size, flexible image correction approach, and improve the efficiency and accuracy of QR code image processing.

Multispectral imaging probe

DOEpatents

Sandison, David R.; Platzbecker, Mark R.; Descour, Michael R.; Armour, David L.; Craig, Marcus J.; Richards-Kortum, Rebecca

1999-01-01

A multispectral imaging probe delivers a range of wavelengths of excitation light to a target and collects a range of expressed light wavelengths. The multispectral imaging probe is adapted for mobile use and use in confined spaces, and is sealed against the effects of hostile environments. The multispectral imaging probe comprises a housing that defines a sealed volume that is substantially sealed from the surrounding environment. A beam splitting device mounts within the sealed volume. Excitation light is directed to the beam splitting device, which directs the excitation light to a target. Expressed light from the target reaches the beam splitting device along a path coaxial with the path traveled by the excitation light from the beam splitting device to the target. The beam splitting device directs expressed light to a collection subsystem for delivery to a detector.

Multispectral imaging probe

DOEpatents

Sandison, D.R.; Platzbecker, M.R.; Descour, M.R.; Armour, D.L.; Craig, M.J.; Richards-Kortum, R.

1999-07-27

A multispectral imaging probe delivers a range of wavelengths of excitation light to a target and collects a range of expressed light wavelengths. The multispectral imaging probe is adapted for mobile use and use in confined spaces, and is sealed against the effects of hostile environments. The multispectral imaging probe comprises a housing that defines a sealed volume that is substantially sealed from the surrounding environment. A beam splitting device mounts within the sealed volume. Excitation light is directed to the beam splitting device, which directs the excitation light to a target. Expressed light from the target reaches the beam splitting device along a path coaxial with the path traveled by the excitation light from the beam splitting device to the target. The beam splitting device directs expressed light to a collection subsystem for delivery to a detector. 8 figs.

Determination of differential cross sections for electron-impact excitation of electronic states of molecular oxygen

NASA Astrophysics Data System (ADS)

Campbell, L.; Green, M. A.; Brunger, M. J.; Teubner, P. J.; Cartwright, D. C.

2000-02-01

The development and initial results of a method for the determination of differential cross sections for electron scattering by molecular oxygen are described. The method has been incorporated into an existing package of computer programs which, given spectroscopic factors, dissociation energies and an energy-loss spectrum for electron-impact excitation, determine the differential cross sections for each electronic state relative to that of the elastic peak. Enhancements of the original code were made to deal with particular aspects of electron scattering from O2, such as the overlap of vibrational levels of the ground state with transitions to excited states, and transitions to levels close to and above the dissocation energy in the Herzberg and Schumann-Runge continua. The utility of the code is specifically demonstrated for the ``6-eV states'' of O2, where we report absolute differential cross sections for their excitation by 15-eV electrons. In addition an integral cross section, derived from the differential cross section measurements, is also reported for this excitation process and compared against available theoretical results. The present differential and integral cross sections for excitation of the ``6-eV states'' of O2 are the first to be reported in the literature for electron-impact energies below 20 eV.

Three-dimensional cell organization leads to almost immediate HRE activity as demonstrated by molecular imaging of MG-63 spheroids using two-photon excitation microscopy.

PubMed

Indovina, Paola; Collini, Maddalena; Chirico, Giuseppe; Santini, Maria Teresa

2007-02-20

Hypoxia through HRE (hypoxia-responsive element) activity in MG-63 human osteosarcoma cells grown in monolayer and as very small, three-dimensional tumor spheroids was investigated using molecular imaging techniques. MG-63 cells were stably transfected with a vector constructed with multiple copies of the HRE sequence of the human vascular endothelial growth factor (VEGF) gene and with the enhanced green fluorescent protein (EGFP) coding sequence. During hypoxia when HIF-1alpha (hypoxia-inducible factor-1alpha) is stabilized, the binding of HIF-1 to the HRE sequences of the vector allows the transcription of EGFP and the appearance of fluorescence. Transfected monolayer cells were characterized by flow cytometric analysis in response to various hypoxic conditions and HIF-1alpha expression in these cells was assessed by Western blotting. Two-photon excitation (TPE) microscopy was then used to examine both MG-63-transfected monolayer cells and spheroids at 2 and 5 days of growth in normoxic conditions. Monolayer cells reveal almost no fluorescence, whereas even very small spheroids (<100 microm) after 2 days of growth contain regions of high fluorescence. For the first time in the literature, at least to our knowledge, it is demonstrated, using highly sensitive and non-perturbing molecular imaging techniques, that three-dimensional cell organization leads to almost immediate HRE activation. This activation of the HRE sequences, which control a wide variety of genes, suggests that monolayer cells and spheroids of the MG-63 cell line have different genes activated and thus diverse functional activities.

Marine EM: The Past, The Present, and The Future

NASA Astrophysics Data System (ADS)

Constable, S.

2016-12-01

The high cost of deepwater exploration motivated the development of commercial marine magnetotelluric (MT) exploration in 1995, but it wasn't until marine controlled-source electromagnetic (CSEM) methods burst upon the industry scene with the formation of three new contractors in 2002 that things got really exciting. Now the bubble has burst and the excitement has diminished, but marine EM remains an important tool for offshore exploration. Early mistakes were made as a result of poor instrumentation and a lack of good interpretation tools - unlike seismics, EM relies heavily on inversion to produce useful results - but both equipment and inversion codes have improved significantly. Still, there are mistakes that can be made. Rock anisotropy and seawater conductivity have to be handled appropriately. A strong galvanic response means that there is a resistivity/thickness ambiguity when imaging reservoirs, but the inductive nature of the data means that multi-frequency inversions are very much better than using single frequencies. Resolution will never be as good as for seismic methods, but is much better than for potential field methods and conductivity may often be a more diagnostic property than acoustic impedance. EM images resistivity, not hydrocarbon content, and false positives occasionally occur, but false negatives are rare. That is, without a CSEM signature there is little chance of discovering economical hydrocarbons. This should bode well for the future of the method.

Interferometric temporal focusing microscopy using three-photon excitation fluorescence.

PubMed

Toda, Keisuke; Isobe, Keisuke; Namiki, Kana; Kawano, Hiroyuki; Miyawaki, Atsushi; Midorikawa, Katsumi

2018-04-01

Super-resolution microscopy has become a powerful tool for biological research. However, its spatial resolution and imaging depth are limited, largely due to background light. Interferometric temporal focusing (ITF) microscopy, which combines structured illumination microscopy and three-photon excitation fluorescence microscopy, can overcome these limitations. Here, we demonstrate ITF microscopy using three-photon excitation fluorescence, which has a spatial resolution of 106 nm at an imaging depth of 100 µm with an excitation wavelength of 1060 nm.

What is special about the adolescent (JME) brain?

PubMed

Craiu, Dana

2013-07-01

Juvenile myoclonic epilepsy (JME) involves cortico-thalamo-cortical networks. Thalamic, frontal gray matter, connectivity, and neurotransmitter disturbances have been demonstrated by structural/functional imaging studies. Few patients with JME show mutations in genes coding ion channels or GABAA (gamma-aminobutyric acid) receptor subunits. Recent research points to EFHC1 gene mutations leading to microdysgenesis and possible aberrant circuitry. Imaging studies have shown massive structural/functional changes of normally developing adolescent brain structures maturing at strikingly different rates and times. Gray matter (GM) volume diminishes in cortical areas (frontal and parietal) and deep structures (anterior thalamus, putamen, and caudate). Diffusion tensor imaging (DTI) findings support continued microstructural change in WM (white matter) during late adolescence with robust developmental changes in thalamocortical connectivity. The GABAA receptor distribution and specific receptor subunits' expression patterns change with age from neonate to adolescent/adult, contributing to age-related changes in brain excitability. Hormonal influence on brain structure development during adolescence is presented. Possible implications of brain changes during adolescence on the course of JME are discussed. Copyright © 2012 Elsevier Inc. All rights reserved.

Two-photon excitation fluorescence bioassays.

PubMed

Hänninen, Pekka; Soukka, Jori; Soini, Juhani T

2008-01-01

Application of two-photon excitation of fluorescence in microscopy is one of the major discoveries of the "renaissance" of light microscopy that started in the 1980s. The technique derives its advantages from the biologically "smooth" wavelength of the excitation light and the confinement of the excitation. Difficult, and seemingly nontransparent, samples may be imaged with the technique with good resolution. Although the bioresearch has been concentrating mostly on the positive properties of the technique for imaging, the same properties may be applied successfully to nonimaging bioassays. This article focuses on the development path of two-photon excitation-based assay system.

Advanced technology development for image gathering, coding, and processing

NASA Technical Reports Server (NTRS)

Huck, Friedrich O.

1990-01-01

Three overlapping areas of research activities are presented: (1) Information theory and optimal filtering are extended to visual information acquisition and processing. The goal is to provide a comprehensive methodology for quantitatively assessing the end-to-end performance of image gathering, coding, and processing. (2) Focal-plane processing techniques and technology are developed to combine effectively image gathering with coding. The emphasis is on low-level vision processing akin to the retinal processing in human vision. (3) A breadboard adaptive image-coding system is being assembled. This system will be used to develop and evaluate a number of advanced image-coding technologies and techniques as well as research the concept of adaptive image coding.

A progressive data compression scheme based upon adaptive transform coding: Mixture block coding of natural images

NASA Technical Reports Server (NTRS)

Rost, Martin C.; Sayood, Khalid

1991-01-01

A method for efficiently coding natural images using a vector-quantized variable-blocksized transform source coder is presented. The method, mixture block coding (MBC), incorporates variable-rate coding by using a mixture of discrete cosine transform (DCT) source coders. Which coders are selected to code any given image region is made through a threshold driven distortion criterion. In this paper, MBC is used in two different applications. The base method is concerned with single-pass low-rate image data compression. The second is a natural extension of the base method which allows for low-rate progressive transmission (PT). Since the base method adapts easily to progressive coding, it offers the aesthetic advantage of progressive coding without incorporating extensive channel overhead. Image compression rates of approximately 0.5 bit/pel are demonstrated for both monochrome and color images.

Dual neutral particle induced transmutation in CINDER2008

NASA Astrophysics Data System (ADS)

Martin, W. J.; de Oliveira, C. R. E.; Hecht, A. A.

2014-12-01

Although nuclear transmutation methods for fission have existed for decades, the focus has been on neutron-induced reactions. Recent novel concepts have sought to use both neutrons and photons for purposes such as active interrogation of cargo to detect the smuggling of highly enriched uranium, a concept that would require modeling the transmutation caused by both incident particles. As photonuclear transmutation has yet to be modeled alongside neutron-induced transmutation in a production code, new methods need to be developed. The CINDER2008 nuclear transmutation code from Los Alamos National Laboratory is extended from neutron applications to dual neutral particle applications, allowing both neutron- and photon-induced reactions for this modeling with a focus on fission. Following standard reaction modeling, the induced fission reaction is understood as a two-part reaction, with an entrance channel to the excited compound nucleus, and an exit channel from the excited compound nucleus to the fission fragmentation. Because photofission yield data-the exit channel from the compound nucleus-are sparse, neutron fission yield data are used in this work. With a different compound nucleus and excitation, the translation to the excited compound state is modified, as appropriate. A verification and validation of these methods and data has been performed. This has shown that the translation of neutron-induced fission product yield sets, and their use in photonuclear applications, is appropriate, and that the code has been extended correctly.

Comparison of excitation wavelengths for in vivo deep imaging of mouse brain

NASA Astrophysics Data System (ADS)

Wang, Mengran; Wu, Chunyan; Li, Bo; Xia, Fei; Sinefeld, David; Xu, Chris

2018-02-01

The attenuation of excitation power reaching the focus is the main issue that limits the depth penetration of highresolution imaging of biological tissue. The attenuation is caused by a combination of tissue scattering and absorption. Theoretical model of the effective attenuation length for in vivo mouse brain imaging has been built based on the data of the absorption of water and blood and the Mie scattering of a tissue-like phantom. Such a theoretical model has been corroborated at a number of excitation wavelengths, such as 800 nm, 1300 nm , and 1700 nm ; however, the attenuation caused by absorption is negligible when compared to tissue scattering at all these wavelength windows. Here we performed in vivo three-photon imaging of Texas Red-stained vasculature in the same mouse brain with different excitation wavelengths, 1700 nm, 1550 nm, 1500 nm and 1450 nm. In particular, our studies include the wavelength regime where strong water absorption is present (i.e., 1450 nm), and the attenuation by water absorption is predicted to be the dominant contribution in the excitation attenuation. Based on the experimental results, we found that the effective attenuation length at 1450 nm is significantly shorter than those at 1700 nm and 1300 nm. Our results confirm that the theoretical model based on tissue scattering and water absorption is accurate in predicting the effective attenuation lengths for in vivo imaging. The optimum excitation wavelength windows for in vivo mouse brain imaging are at 1300 nm and 1700 nm.

Whole body traveling wave magnetic resonance imaging at high field strength: homogeneity, efficiency, and energy deposition as compared with traditional excitation mechanisms.

PubMed

Zhang, Bei; Sodickson, Daniel K; Lattanzi, Riccardo; Duan, Qi; Stoeckel, Bernd; Wiggins, Graham C

2012-04-01

In 7 T traveling wave imaging, waveguide modes supported by the scanner radiofrequency shield are used to excite an MR signal in samples or tissue which may be several meters away from the antenna used to drive radiofrequency power into the system. To explore the potential merits of traveling wave excitation for whole-body imaging at 7 T, we compare numerical simulations of traveling wave and TEM systems, and juxtapose full-wave electrodynamic simulations using a human body model with in vivo human traveling wave imaging at multiple stations covering the entire body. The simulated and in vivo traveling wave results correspond well, with strong signal at the periphery of the body and weak signal deep in the torso. These numerical results also illustrate the complicated wave behavior that emerges when a body is present. The TEM resonator simulation allowed comparison of traveling wave excitation with standard quadrature excitation, showing that while the traveling wave B +1 per unit drive voltage is much less than that of the TEM system, the square of the average B +1 compared to peak specific absorption rate (SAR) values can be comparable in certain imaging planes. Both systems produce highly inhomogeneous excitation of MR signal in the torso, suggesting that B(1) shimming or other parallel transmission methods are necessary for 7 T whole body imaging. Copyright © 2011 Wiley-Liss, Inc.

Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy

PubMed Central

Clendenon, Sherry G.; Young, Pamela A.; Ferkowicz, Michael; Phillips, Carrie; Dunn, Kenneth W.

2015-01-01

In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging. PMID:21729357

Magneto-acoustic imaging by continuous-wave excitation.

PubMed

Shunqi, Zhang; Zhou, Xiaoqing; Tao, Yin; Zhipeng, Liu

2017-04-01

The electrical characteristics of tissue yield valuable information for early diagnosis of pathological changes. Magneto-acoustic imaging is a functional approach for imaging of electrical conductivity. This study proposes a continuous-wave magneto-acoustic imaging method. A kHz-range continuous signal with an amplitude range of several volts is used to excite the magneto-acoustic signal and improve the signal-to-noise ratio. The magneto-acoustic signal amplitude and phase are measured to locate the acoustic source via lock-in technology. An optimisation algorithm incorporating nonlinear equations is used to reconstruct the magneto-acoustic source distribution based on the measured amplitude and phase at various frequencies. Validation simulations and experiments were performed in pork samples. The experimental and simulation results agreed well. While the excitation current was reduced to 10 mA, the acoustic signal magnitude increased up to 10 -7  Pa. Experimental reconstruction of the pork tissue showed that the image resolution reached mm levels when the excitation signal was in the kHz range. The signal-to-noise ratio of the detected magneto-acoustic signal was improved by more than 25 dB at 5 kHz when compared to classical 1 MHz pulse excitation. The results reported here will aid further research into magneto-acoustic generation mechanisms and internal tissue conductivity imaging.

Binary video codec for data reduction in wireless visual sensor networks

NASA Astrophysics Data System (ADS)

Khursheed, Khursheed; Ahmad, Naeem; Imran, Muhammad; O'Nils, Mattias

2013-02-01

Wireless Visual Sensor Networks (WVSN) is formed by deploying many Visual Sensor Nodes (VSNs) in the field. Typical applications of WVSN include environmental monitoring, health care, industrial process monitoring, stadium/airports monitoring for security reasons and many more. The energy budget in the outdoor applications of WVSN is limited to the batteries and the frequent replacement of batteries is usually not desirable. So the processing as well as the communication energy consumption of the VSN needs to be optimized in such a way that the network remains functional for longer duration. The images captured by VSN contain huge amount of data and require efficient computational resources for processing the images and wide communication bandwidth for the transmission of the results. Image processing algorithms must be designed and developed in such a way that they are computationally less complex and must provide high compression rate. For some applications of WVSN, the captured images can be segmented into bi-level images and hence bi-level image coding methods will efficiently reduce the information amount in these segmented images. But the compression rate of the bi-level image coding methods is limited by the underlined compression algorithm. Hence there is a need for designing other intelligent and efficient algorithms which are computationally less complex and provide better compression rate than that of bi-level image coding methods. Change coding is one such algorithm which is computationally less complex (require only exclusive OR operations) and provide better compression efficiency compared to image coding but it is effective for applications having slight changes between adjacent frames of the video. The detection and coding of the Region of Interest (ROIs) in the change frame efficiently reduce the information amount in the change frame. But, if the number of objects in the change frames is higher than a certain level then the compression efficiency of both the change coding and ROI coding becomes worse than that of image coding. This paper explores the compression efficiency of the Binary Video Codec (BVC) for the data reduction in WVSN. We proposed to implement all the three compression techniques i.e. image coding, change coding and ROI coding at the VSN and then select the smallest bit stream among the results of the three compression techniques. In this way the compression performance of the BVC will never become worse than that of image coding. We concluded that the compression efficiency of BVC is always better than that of change coding and is always better than or equal that of ROI coding and image coding.

Time-resolved multicolor two-photon excitation fluorescence microscopy of cells and tissues

NASA Astrophysics Data System (ADS)

Zheng, Wei

2014-11-01

Multilabeling which maps the distribution of different targets is an indispensable technique in many biochemical and biophysical studies. Two-photon excitation fluorescence (TPEF) microscopy of endogenous fluorophores combining with conventional fluorescence labeling techniques such as genetically encoded fluorescent protein (FP) and fluorescent dyes staining could be a powerful tool for imaging living cells. However, the challenge is that the excitation and emission wavelength of these endogenous fluorophores and fluorescent labels are very different. A multi-color ultrafast source is required for the excitation of multiple fluorescence molecules. In this study, we developed a two-photon imaging system with excitations from the pump femtosecond laser and the selected supercontinuum generated from a photonic crystal fiber (PCF). Multiple endogenous fluorophores, fluorescent proteins and fluorescent dyes were excited in their optimal wavelengths simultaneously. A time- and spectral-resolved detection system was used to record the TPEF signals. This detection technique separated the TPEF signals from multiple sources in time and wavelength domains. Cellular organelles such as nucleus, mitochondria, microtubule and endoplasmic reticulum, were clearly revealed in the TPEF images. The simultaneous imaging of multiple fluorophores of cells will greatly aid the study of sub-cellular compartments and protein localization.

Scalable Coding of Plenoptic Images by Using a Sparse Set and Disparities.

PubMed

Li, Yun; Sjostrom, Marten; Olsson, Roger; Jennehag, Ulf

2016-01-01

One of the light field capturing techniques is the focused plenoptic capturing. By placing a microlens array in front of the photosensor, the focused plenoptic cameras capture both spatial and angular information of a scene in each microlens image and across microlens images. The capturing results in a significant amount of redundant information, and the captured image is usually of a large resolution. A coding scheme that removes the redundancy before coding can be of advantage for efficient compression, transmission, and rendering. In this paper, we propose a lossy coding scheme to efficiently represent plenoptic images. The format contains a sparse image set and its associated disparities. The reconstruction is performed by disparity-based interpolation and inpainting, and the reconstructed image is later employed as a prediction reference for the coding of the full plenoptic image. As an outcome of the representation, the proposed scheme inherits a scalable structure with three layers. The results show that plenoptic images are compressed efficiently with over 60 percent bit rate reduction compared with High Efficiency Video Coding intra coding, and with over 20 percent compared with an High Efficiency Video Coding block copying mode.

Information retrieval based on single-pixel optical imaging with quick-response code

NASA Astrophysics Data System (ADS)

Xiao, Yin; Chen, Wen

2018-04-01

Quick-response (QR) code technique is combined with ghost imaging (GI) to recover original information with high quality. An image is first transformed into a QR code. Then the QR code is treated as an input image in the input plane of a ghost imaging setup. After measurements, traditional correlation algorithm of ghost imaging is utilized to reconstruct an image (QR code form) with low quality. With this low-quality image as an initial guess, a Gerchberg-Saxton-like algorithm is used to improve its contrast, which is actually a post processing. Taking advantage of high error correction capability of QR code, original information can be recovered with high quality. Compared to the previous method, our method can obtain a high-quality image with comparatively fewer measurements, which means that the time-consuming postprocessing procedure can be avoided to some extent. In addition, for conventional ghost imaging, the larger the image size is, the more measurements are needed. However, for our method, images with different sizes can be converted into QR code with the same small size by using a QR generator. Hence, for the larger-size images, the time required to recover original information with high quality will be dramatically reduced. Our method makes it easy to recover a color image in a ghost imaging setup, because it is not necessary to divide the color image into three channels and respectively recover them.

Cross-Sectional Transport Imaging in a Multijunction Solar Cell

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haegel, Nancy M.; Ke, Chi-Wen; Taha, Hesham

2015-06-14

Combining highly localized electron-beam excitation at a point with the spatial resolution capability of optical near-field imaging, we have imaged carrier transport in a cross-sectioned multijunction (GaInP/GaInAs/Ge) solar cell. We image energy transport associated with carrier diffusion throughout the full width of the middle (GaInAs) cell and luminescent coupling from point excitation in the top cell GaInP to the middle cell. Supporting cathodoluminescence and near-field photoluminescence measurements demonstrate excitation-dependent Fermi level splitting effects that influence cross-sectioned spectroscopy results as well as transport limitations on the spatial resolution of cross-sectional measurements.

Optimization of the excitation light sheet in selective plane illumination microscopy

PubMed Central

Gao, Liang

2015-01-01

Selective plane illumination microscopy (SPIM) allows rapid 3D live fluorescence imaging on biological specimens with high 3D spatial resolution, good optical sectioning capability and minimal photobleaching and phototoxic effect. SPIM gains its advantage by confining the excitation light near the detection focal plane, and its performance is determined by the ability to create a thin, large and uniform excitation light sheet. Several methods have been developed to create such an excitation light sheet for SPIM. However, each method has its own strengths and weaknesses, and tradeoffs must be made among different aspects in SPIM imaging. In this work, we present a strategy to select the excitation light sheet among the latest SPIM techniques, and to optimize its geometry based on spatial resolution, field of view, optical sectioning capability, and the sample to be imaged. Besides the light sheets discussed in this work, the proposed strategy is also applicable to estimate the SPIM performance using other excitation light sheets. PMID:25798312

Fast ITTBC using pattern code on subband segmentation

NASA Astrophysics Data System (ADS)

Koh, Sung S.; Kim, Hanchil; Lee, Kooyoung; Kim, Hongbin; Jeong, Hun; Cho, Gangseok; Kim, Chunghwa

2000-06-01

Iterated Transformation Theory-Based Coding suffers from very high computational complexity in encoding phase. This is due to its exhaustive search. In this paper, our proposed image coding algorithm preprocess an original image to subband segmentation image by wavelet transform before image coding to reduce encoding complexity. A similar block is searched by using the 24 block pattern codes which are coded by the edge information in the image block on the domain pool of the subband segmentation. As a result, numerical data shows that the encoding time of the proposed coding method can be reduced to 98.82% of that of Joaquin's method, while the loss in quality relative to the Jacquin's is about 0.28 dB in PSNR, which is visually negligible.

Statistical image segmentation for the detection of skin lesion borders in UV fluorescence excitation

NASA Astrophysics Data System (ADS)

Ortega-Martinez, Antonio; Padilla-Martinez, Juan Pablo; Franco, Walfre

2016-04-01

The skin contains several fluorescent molecules or fluorophores that serve as markers of structure, function and composition. UV fluorescence excitation photography is a simple and effective way to image specific intrinsic fluorophores, such as the one ascribed to tryptophan which emits at a wavelength of 345 nm upon excitation at 295 nm, and is a marker of cellular proliferation. Earlier, we built a clinical UV photography system to image cellular proliferation. In some samples, the naturally low intensity of the fluorescence can make it difficult to separate the fluorescence of cells in higher proliferation states from background fluorescence and other imaging artifacts -- like electronic noise. In this work, we describe a statistical image segmentation method to separate the fluorescence of interest. Statistical image segmentation is based on image averaging, background subtraction and pixel statistics. This method allows to better quantify the intensity and surface distributions of fluorescence, which in turn simplify the detection of borders. Using this method we delineated the borders of highly-proliferative skin conditions and diseases, in particular, allergic contact dermatitis, psoriatic lesions and basal cell carcinoma. Segmented images clearly define lesion borders. UV fluorescence excitation photography along with statistical image segmentation may serve as a quick and simple diagnostic tool for clinicians.

Dual wavelength laser diode excitation source for 2D photoacoustic imaging.

NASA Astrophysics Data System (ADS)

Allen, Thomas J.; Beard, Paul C.

2007-02-01

Photoacoustic methods can be used to make spatially resolved spectroscopic measurements of blood oxygenation when using a multiwavelength excitation source, such as an OPO system. Since these excitation sources are usually expensive and bulky, an alternative is to use laser diodes. A fibre coupled laser diode excitation system has been developed, providing two wavelengths, 850 and 905nm, each composed of 6 high peak power pulsed laser diodes. The system provided variable pulse durations (65-500ns) and repetition rates of up to 5KHz. The pulse energies delivered by the excitation system at 905nm and 850nm were measured to be 120μJ and 80μJ respectively for a 200ns pulse duration. To demonstrate the utility of the system, the excitation source was combined with an ultrasound detector to form a probe for in vivo single point measurements of superficial blood vessels. Changes in blood oxygenation and volume in the finger tip were monitored while making venous and arterial occlusions. To demonstrate the imaging capability of the excitation system, 2D photoacoustic images of a physiologically realistic phantom were obtained for a range of pulse durations using a cylindrical scanning system. The phantom was composed of cylindrical absorbing elements (μa=1mm^{-1}) of 2.7mm diameter, immersed in a 1% intralipid solution (μs=1mm^{-1}). This study demonstrated the potential use of laser diodes as an excitation source for photoacoustic imaging of superficial vascular structures.

Separating Bulk and Surface Contributions to Electronic Excited-State Processes in Hybrid Mixed Perovskite Thin Films via Multimodal All-Optical Imaging.

PubMed

Simpson, Mary Jane; Doughty, Benjamin; Das, Sanjib; Xiao, Kai; Ma, Ying-Zhong

2017-07-20

A comprehensive understanding of electronic excited-state phenomena underlying the impressive performance of solution-processed hybrid halide perovskite solar cells requires access to both spatially resolved electronic processes and corresponding sample morphological characteristics. Here, we demonstrate an all-optical multimodal imaging approach that enables us to obtain both electronic excited-state and morphological information on a single optical microscope platform with simultaneous high temporal and spatial resolution. Specifically, images were acquired for the same region of interest in thin films of chloride containing mixed lead halide perovskites (CH 3 NH 3 PbI 3-x Cl x ) using femtosecond transient absorption, time-integrated photoluminescence, confocal reflectance, and transmission microscopies. Comprehensive image analysis revealed the presence of surface- and bulk-dominated contributions to the various images, which describe either spatially dependent electronic excited-state properties or morphological variations across the probed region of the thin films. These results show that PL probes effectively the species near or at the film surface.

Method and apparatus for imaging a sample on a device

DOEpatents

Trulson, Mark; Stern, David; Fiekowsky, Peter; Rava, Richard; Walton, Ian; Fodor, Stephen P. A.

2001-01-01

A method and apparatus for imaging a sample are provided. An electromagnetic radiation source generates excitation radiation which is sized by excitation optics to a line. The line is directed at a sample resting on a support and excites a plurality of regions on the sample. Collection optics collect response radiation reflected from the sample I and image the reflected radiation. A detector senses the reflected radiation and is positioned to permit discrimination between radiation reflected from a certain focal plane in the sample and certain other planes within the sample.

Influence of nuclear de-excitation on observables relevant for space exploration

NASA Astrophysics Data System (ADS)

Mancusi, Davide; Boudard, Alain; Cugnon, Joseph; David, Jean-Christophe; Leray, Sylvie

The composition of the space radiation environment inside spacecrafts is modified by the inter-action with shielding material, with equipment and even with the astronauts' bodies. Accurate quantitative estimates of the effects of nuclear reactions are necessary, for example, for dose estimation and prediction of single-event upset rates. To this end, it is necessary to construct predictive models for nuclear reactions, which usually consist of an intranuclear-cascade or quantum-molecular-dynamics stage, followed by a nuclear de-excitation stage. While it is generally acknowledged that it is necessary to accurately simulate the first reaction stage, transport-code users often neglect or underestimate the importance of the choice of the de-excitation code. The purpose of this work is to prove that the de-excitation model is in fact a non-negligible source of uncertainty for the prediction of several observables of crucial importance for space applications. For some particular observables, such as fragmentation cross sections, the systematic uncertainty due to the de-excitation model actually dominates the theoretical error. Our point will be illustrated by making use of calculations performed with several intranuclear-cascade/de-excitation models, such as the Li`ge Intranuclear Cascade model (INCL) and Isabel (for the cascade part) and ABLA, GEMINI++ and SMM (on the de-excitation side). We will also rely on the results of the recent IAEA intercomparison of spallation models, which can be used as informative groundwork for the evaluation of the global uncertainties involved in nucleon-nucleus reactions.

Staggering of angular momentum distribution in fission

NASA Astrophysics Data System (ADS)

Tamagno, Pierre; Litaize, Olivier

2018-03-01

We review here the role of angular momentum distributions in the fission process. To do so the algorithm implemented in the FIFRELIN code [?] is detailed with special emphasis on the place of fission fragment angular momenta. The usual Rayleigh distribution used for angular momentum distribution is presented and the related model derivation is recalled. Arguments are given to justify why this distribution should not hold for low excitation energy of the fission fragments. An alternative ad hoc expression taking into account low-lying collectiveness is presented as has been implemented in the FIFRELIN code. Yet on observables currently provided by the code, no dramatic impact has been found. To quantify the magnitude of the impact of the low-lying staggering in the angular momentum distribution, a textbook case is considered for the decay of the 144Ba nucleus with low excitation energy.

Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS

PubMed Central

Manno, Carlo; Figueroa, Lourdes; Fitts, Robert

2013-01-01

Imaging, optical mapping, and optical multisite recording of transmembrane potential (Vm) are essential for studying excitable cells and systems. The naphthylstyryl voltage-sensitive dyes, including di-8-ANEPPS, shift both their fluorescence excitation and emission spectra upon changes in Vm. Accordingly, they have been used for monitoring Vm in nonratioing and both emission and excitation ratioing modes. Their changes in fluorescence are usually much less than 10% per 100 mV. Conventional ratioing increases sensitivity to between 3 and 15% per 100 mV. Low sensitivity limits the value of these dyes, especially when imaged with low light systems like confocal scanners. Here we demonstrate the improvement afforded by shifted excitation and emission ratioing (SEER) as applied to imaging membrane potential in flexor digitorum brevis muscle fibers of adult mice. SEER—the ratioing of two images of fluorescence, obtained with different excitation wavelengths in different emission bands—was implemented in two commercial confocal systems. A conventional pinhole scanner, affording optimal setting of emission bands but less than ideal excitation wavelengths, achieved a sensitivity of up to 27% per 100 mV, nearly doubling the value found by conventional ratioing of the same data. A better pair of excitation lights should increase the sensitivity further, to 35% per 100 mV. The maximum acquisition rate with this system was 1 kHz. A fast “slit scanner” increased the effective rate to 8 kHz, but sensitivity was lower. In its high-sensitivity implementation, the technique demonstrated progressive deterioration of action potentials upon fatiguing tetani induced by stimulation patterns at >40 Hz, thereby identifying action potential decay as a contributor to fatigue onset. Using the fast implementation, we could image for the first time an action potential simultaneously at multiple locations along the t-tubule system. These images resolved the radially varying lag associated with propagation at a finite velocity. PMID:23440278

Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS.

PubMed

Manno, Carlo; Figueroa, Lourdes; Fitts, Robert; Ríos, Eduardo

2013-03-01

Imaging, optical mapping, and optical multisite recording of transmembrane potential (V(m)) are essential for studying excitable cells and systems. The naphthylstyryl voltage-sensitive dyes, including di-8-ANEPPS, shift both their fluorescence excitation and emission spectra upon changes in V(m). Accordingly, they have been used for monitoring V(m) in nonratioing and both emission and excitation ratioing modes. Their changes in fluorescence are usually much less than 10% per 100 mV. Conventional ratioing increases sensitivity to between 3 and 15% per 100 mV. Low sensitivity limits the value of these dyes, especially when imaged with low light systems like confocal scanners. Here we demonstrate the improvement afforded by shifted excitation and emission ratioing (SEER) as applied to imaging membrane potential in flexor digitorum brevis muscle fibers of adult mice. SEER--the ratioing of two images of fluorescence, obtained with different excitation wavelengths in different emission bands-was implemented in two commercial confocal systems. A conventional pinhole scanner, affording optimal setting of emission bands but less than ideal excitation wavelengths, achieved a sensitivity of up to 27% per 100 mV, nearly doubling the value found by conventional ratioing of the same data. A better pair of excitation lights should increase the sensitivity further, to 35% per 100 mV. The maximum acquisition rate with this system was 1 kHz. A fast "slit scanner" increased the effective rate to 8 kHz, but sensitivity was lower. In its high-sensitivity implementation, the technique demonstrated progressive deterioration of action potentials upon fatiguing tetani induced by stimulation patterns at >40 Hz, thereby identifying action potential decay as a contributor to fatigue onset. Using the fast implementation, we could image for the first time an action potential simultaneously at multiple locations along the t-tubule system. These images resolved the radially varying lag associated with propagation at a finite velocity.

Twisted bilayer graphene photoluminescence emission peaks at van Hove singularities.

PubMed

Alencar, Thonimar V; von Dreifus, Driele; Gabriela Cota Moreira, Maria; Eliel, Gomes S N; Yeh, Chao-Hui; Chiu, Po-Wen; Pimenta, Marcos A; Malard, Leandro M; Maria de Paula, Ana

2018-05-02

We report on photoluminescence emission imaging by femtosecond laser excitation on twisted bilayer graphene samples. The emission images are obtained by tuning the excitation laser energies in the near infrared region. We demonstrate an increase of the photoluminescence emission at excitation energies that depends on the bilayer twist angle. The results show a peak for the light emission when the excitation is in resonance with transitions at the van Hove singularities in the electronic density of states. We measured the photoluminescence excitation peak position and width for samples with various twist angles showing resonances in the energy range of 1.2 to 1.7 eV.

Twisted bilayer graphene photoluminescence emission peaks at van Hove singularities

NASA Astrophysics Data System (ADS)

Alencar, Thonimar V.; von Dreifus, Driele; Cota Moreira, Maria Gabriela; Eliel, Gomes S. N.; Yeh, Chao-Hui; Chiu, Po-Wen; Pimenta, Marcos A.; Malard, Leandro M.; de Paula, Ana Maria

2018-05-01

We report on photoluminescence emission imaging by femtosecond laser excitation on twisted bilayer graphene samples. The emission images are obtained by tuning the excitation laser energies in the near infrared region. We demonstrate an increase of the photoluminescence emission at excitation energies that depends on the bilayer twist angle. The results show a peak for the light emission when the excitation is in resonance with transitions at the van Hove singularities in the electronic density of states. We measured the photoluminescence excitation peak position and width for samples with various twist angles showing resonances in the energy range of 1.2 to 1.7 eV.

X-Ray Sum Frequency Diffraction for Direct Imaging of Ultrafast Electron Dynamics

NASA Astrophysics Data System (ADS)

Rouxel, Jérémy R.; Kowalewski, Markus; Bennett, Kochise; Mukamel, Shaul

2018-06-01

X-ray diffraction from molecules in the ground state produces an image of their charge density, and time-resolved x-ray diffraction can thus monitor the motion of the nuclei. However, the density change of excited valence electrons upon optical excitation can barely be monitored with regular diffraction techniques due to the overwhelming background contribution of the core electrons. We present a nonlinear x-ray technique made possible by novel free electron laser sources, which provides a spatial electron density image of valence electron excitations. The technique, sum frequency generation carried out with a visible pump and a broadband x-ray diffraction pulse, yields snapshots of the transition charge densities, which represent the electron density variations upon optical excitation. The technique is illustrated by ab initio simulations of transition charge density imaging for the optically induced electronic dynamics in a donor or acceptor substituted stilbene.

Preliminary study of diagnostic spectroscopic imaging for nasopharyngeal carcinoma

NASA Astrophysics Data System (ADS)

Li, Buhong; Xie, Shusen; Zhang, Xiaodong; Li, Depin

2003-12-01

The optical biopsy system for nasopharyngeal carcinoma based on the technique of laser-induced exogenous fluorescence has been successful developed. Ar+ laser was selected as the excitation light source based on the measurement of the Emission-Excitation Matrix of Hematoporphyrin Monomethyl Ether. Tissue-simulating optical phantoms diluted with different concentration of HMME were used to simulated nasopharyngeal carcinoma lesions in the performance test for the drug-fluorescence optical biopsy system, especially for the comparison of fluorescence image contrast between the excitation wavelength of 488nm and 514.5nm, respectively. Experimental results show that the fluorescence image contrast of simulated nasopharyngeal carcinoma lesions excited by the light at the wavelength of 488nm is about three fold higher than that at 514.5nm, and the sensitivity and resolution of the fluorescence and reflection twilight image can satisfy the needs for clinical diagnosis and localization.

Multiple excitation nano-spot generation and confocal detection for far-field microscopy.

PubMed

Mondal, Partha Pratim

2010-03-01

An imaging technique is developed for the controlled generation of multiple excitation nano-spots for far-field microscopy. The system point spread function (PSF) is obtained by interfering two counter-propagating extended depth-of-focus PSF (DoF-PSF), resulting in highly localized multiple excitation spots along the optical axis. The technique permits (1) simultaneous excitation of multiple planes in the specimen; (2) control of the number of spots by confocal detection; and (3) overcoming the point-by-point based excitation. Fluorescence detection from the excitation spots can be efficiently achieved by Z-scanning the detector/pinhole assembly. The technique complements most of the bioimaging techniques and may find potential application in high resolution fluorescence microscopy and nanoscale imaging.

Multiple excitation nano-spot generation and confocal detection for far-field microscopy

NASA Astrophysics Data System (ADS)

Mondal, Partha Pratim

2010-03-01

An imaging technique is developed for the controlled generation of multiple excitation nano-spots for far-field microscopy. The system point spread function (PSF) is obtained by interfering two counter-propagating extended depth-of-focus PSF (DoF-PSF), resulting in highly localized multiple excitation spots along the optical axis. The technique permits (1) simultaneous excitation of multiple planes in the specimen; (2) control of the number of spots by confocal detection; and (3) overcoming the point-by-point based excitation. Fluorescence detection from the excitation spots can be efficiently achieved by Z-scanning the detector/pinhole assembly. The technique complements most of the bioimaging techniques and may find potential application in high resolution fluorescence microscopy and nanoscale imaging.

Discrete Cosine Transform Image Coding With Sliding Block Codes

NASA Astrophysics Data System (ADS)

Divakaran, Ajay; Pearlman, William A.

1989-11-01

A transform trellis coding scheme for images is presented. A two dimensional discrete cosine transform is applied to the image followed by a search on a trellis structured code. This code is a sliding block code that utilizes a constrained size reproduction alphabet. The image is divided into blocks by the transform coding. The non-stationarity of the image is counteracted by grouping these blocks in clusters through a clustering algorithm, and then encoding the clusters separately. Mandela ordered sequences are formed from each cluster i.e identically indexed coefficients from each block are grouped together to form one dimensional sequences. A separate search ensues on each of these Mandela ordered sequences. Padding sequences are used to improve the trellis search fidelity. The padding sequences absorb the error caused by the building up of the trellis to full size. The simulations were carried out on a 256x256 image ('LENA'). The results are comparable to any existing scheme. The visual quality of the image is enhanced considerably by the padding and clustering.

Applications of two-photon fluorescence microscopy in deep-tissue imaging

NASA Astrophysics Data System (ADS)

Dong, Chen-Yuan; Yu, Betty; Hsu, Lily L.; Kaplan, Peter D.; Blankschstein, D.; Langer, Robert; So, Peter T. C.

2000-07-01

Based on the non-linear excitation of fluorescence molecules, two-photon fluorescence microscopy has become a significant new tool for biological imaging. The point-like excitation characteristic of this technique enhances image quality by the virtual elimination of off-focal fluorescence. Furthermore, sample photodamage is greatly reduced because fluorescence excitation is limited to the focal region. For deep tissue imaging, two-photon microscopy has the additional benefit in the greatly improved imaging depth penetration. Since the near- infrared laser sources used in two-photon microscopy scatter less than their UV/glue-green counterparts, in-depth imaging of highly scattering specimen can be greatly improved. In this work, we will present data characterizing both the imaging characteristics (point-spread-functions) and tissue samples (skin) images using this novel technology. In particular, we will demonstrate how blind deconvolution can be used further improve two-photon image quality and how this technique can be used to study mechanisms of chemically-enhanced, transdermal drug delivery.

Excitation functions of the natCr(p,x)44Ti, 56Fe(p,x)44Ti, natNi(p,x)44Ti and 93Nb(p,x)44Ti reactions at energies up to 2.6 GeV

NASA Astrophysics Data System (ADS)

Titarenko, Yu. E.; Batyaev, V. F.; Pavlov, K. V.; Titarenko, A. Yu.; Zhivun, V. M.; Chauzova, M. V.; Balyuk, S. A.; Bebenin, P. V.; Ignatyuk, A. V.; Mashnik, S. G.; Leray, S.; Boudard, A.; David, J. C.; Mancusi, D.; Cugnon, J.; Yariv, Y.; Nishihara, K.; Matsuda, N.; Kumawat, H.; Stankovskiy, A. Yu.

2016-06-01

The paper presents the measured cumulative yields of 44Ti for natCr, 56Fe, natNi and 93Nb samples irradiated by protons at the energy range 0.04-2.6 GeV. The obtained excitation functions are compared with calculations of the well-known codes: ISABEL, Bertini, INCL4.2+ABLA, INCL4.5+ABLA07, PHITS, CASCADE07 and CEM03.02. The predictive power of these codes regarding the studied nuclides is analyzed.

An analytical study on excitation of nuclear-coupled thermal-hydraulic instability due to seismically induced resonance in BWR

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hirano, Masashi

1997-07-01

This paper describes the results of a scoping study on seismically induced resonance of nuclear-coupled thermal-hydraulic instability in BWRs, which was conducted by using TRAC-BF1 within a framework of a point kinetics model. As a result of the analysis, it is shown that a reactivity insertion could occur accompanied by in-surge of coolant into the core resulted from the excitation of the nuclear-coupled instability by the external acceleration. In order to analyze this phenomenon more in detail, it is necessary to couple a thermal-hydraulic code with a three-dimensional nuclear kinetics code.

Integrated smartphone imaging of quantum dot photoluminescence and Förster resonance energy transfer

NASA Astrophysics Data System (ADS)

Petryayeva, Eleonora; Algar, W. Russ

2015-06-01

Smartphones and other mobile devices are emerging as promising analytical platforms for point-of-care diagnostics, particularly when combined with nanotechnology. For example, we have shown that the optical properties of semiconductor quantum dots (QDs) are well suited to photoluminescence (PL) detection with a smartphone camera. However, this previous work has utilized an external excitation source for interrogation of QD PL. In this proceeding, we demonstrate that the white-light LED photographic flashes built into smartphones can be optically filtered to yield blue light suitable for excitation of QD PL. Measurements were made by recording video with filtered flash illumination and averaging the frames of the video to obtain images with good signal-to-background ratios. These images permitted detection of green-emitting and red-emitting QDs at levels comparable to those possible with excitation using an external long-wave UV lamp. The optical properties of QDs proved to be uniquely suited to smartphone PL imaging, exhibiting emission that was 1-2 orders magnitude brighter than that of common fluorescent dyes under the same conditions. Excitation with the smartphone flash was also suitable for imaging of FRET between green-emitting QD donors and Alexa Fluor 555 (A555) fluorescent dye acceptors. No significant difference in FRET imaging capability was observed between excitation with the smartphone flash and a long-wave UV lamp. Although the smartphone flash did have some disadvantages compared to an external UV lamp, these disadvantages are potentially offset by the benefit of having excitation and detection integrated into the smartphone.

Displaying radiologic images on personal computers: image storage and compression--Part 2.

PubMed

Gillespy, T; Rowberg, A H

1994-02-01

This is part 2 of our article on image storage and compression, the third article of our series for radiologists and imaging scientists on displaying, manipulating, and analyzing radiologic images on personal computers. Image compression is classified as lossless (nondestructive) or lossy (destructive). Common lossless compression algorithms include variable-length bit codes (Huffman codes and variants), dictionary-based compression (Lempel-Ziv variants), and arithmetic coding. Huffman codes and the Lempel-Ziv-Welch (LZW) algorithm are commonly used for image compression. All of these compression methods are enhanced if the image has been transformed into a differential image based on a differential pulse-code modulation (DPCM) algorithm. The LZW compression after the DPCM image transformation performed the best on our example images, and performed almost as well as the best of the three commercial compression programs tested. Lossy compression techniques are capable of much higher data compression, but reduced image quality and compression artifacts may be noticeable. Lossy compression is comprised of three steps: transformation, quantization, and coding. Two commonly used transformation methods are the discrete cosine transformation and discrete wavelet transformation. In both methods, most of the image information is contained in a relatively few of the transformation coefficients. The quantization step reduces many of the lower order coefficients to 0, which greatly improves the efficiency of the coding (compression) step. In fractal-based image compression, image patterns are stored as equations that can be reconstructed at different levels of resolution.

A blind dual color images watermarking based on IWT and state coding

NASA Astrophysics Data System (ADS)

Su, Qingtang; Niu, Yugang; Liu, Xianxi; Zhu, Yu

2012-04-01

In this paper, a state-coding based blind watermarking algorithm is proposed to embed color image watermark to color host image. The technique of state coding, which makes the state code of data set be equal to the hiding watermark information, is introduced in this paper. When embedding watermark, using Integer Wavelet Transform (IWT) and the rules of state coding, these components, R, G and B, of color image watermark are embedded to these components, Y, Cr and Cb, of color host image. Moreover, the rules of state coding are also used to extract watermark from the watermarked image without resorting to the original watermark or original host image. Experimental results show that the proposed watermarking algorithm cannot only meet the demand on invisibility and robustness of the watermark, but also have well performance compared with other proposed methods considered in this work.

Cardiac imaging at 7 Tesla: Single- and two-spoke radiofrequency pulse design with 16-channel parallel excitation.

PubMed

Schmitter, Sebastian; DelaBarre, Lance; Wu, Xiaoping; Greiser, Andreas; Wang, Dingxin; Auerbach, Edward J; Vaughan, J Thomas; Uğurbil, Kâmil; Van de Moortele, Pierre-François

2013-11-01

Higher signal to noise ratio (SNR) and improved contrast have been demonstrated at ultra-high magnetic fields (≥7 Tesla [T]) in multiple targets, often with multi-channel transmit methods to address the deleterious impact on tissue contrast due to spatial variations in B1 (+) profiles. When imaging the heart at 7T, however, respiratory and cardiac motion, as well as B0 inhomogeneity, greatly increase the methodological challenge. In this study we compare two-spoke parallel transmit (pTX) RF pulses with static B1 (+) shimming in cardiac imaging at 7T. Using a 16-channel pTX system, slice-selective two-spoke pTX pulses and static B1 (+) shimming were applied in cardiac CINE imaging. B1 (+) and B0 mapping required modified cardiac triggered sequences. Excitation homogeneity and RF energy were compared in different imaging orientations. Two-spoke pulses provide higher excitation homogeneity than B1 (+) shimming, especially in the more challenging posterior region of the heart. The peak value of channel-wise RF energy was reduced, allowing for a higher flip angle, hence increased tissue contrast. Image quality with two-spoke excitation proved to be stable throughout the entire cardiac cycle. Two-spoke pTX excitation has been successfully demonstrated in the human heart at 7T, with improved image quality and reduced RF pulse energy when compared with B1 (+) shimming. Copyright © 2013 Wiley Periodicals, Inc.

Document image retrieval through word shape coding.

PubMed

Lu, Shijian; Li, Linlin; Tan, Chew Lim

2008-11-01

This paper presents a document retrieval technique that is capable of searching document images without OCR (optical character recognition). The proposed technique retrieves document images by a new word shape coding scheme, which captures the document content through annotating each word image by a word shape code. In particular, we annotate word images by using a set of topological shape features including character ascenders/descenders, character holes, and character water reservoirs. With the annotated word shape codes, document images can be retrieved by either query keywords or a query document image. Experimental results show that the proposed document image retrieval technique is fast, efficient, and tolerant to various types of document degradation.

Cutaneous porphyrins exhibit anti-stokes fluorescence that is detectable in sebum (Conference Presentation)

NASA Astrophysics Data System (ADS)

Tian, Giselle; Zeng, Haishan; Zhao, Jianhua; Wu, Zhenguo; Al Jasser, Mohammed; Lui, Harvey; Mclean, David I.

2016-02-01

Porphyrins produced by Propionibacterium acnes represent the principal fluorophore associated with acne, and appear as orange-red luminescence under the Wood's lamp. Assessment of acne based on Wood's lamp (UV) or visible light illumination is limited by photon penetration depth and has limited sensitivity for earlier stage lesions. Inducing fluorescence with near infrared (NIR) excitation may provide an alternative way to assess porphyrin-related skin disorders. We discovered that under 785 nm CW laser excitation PpIX powder exhibits fluorescence emission in the shorter wavelength range of 600-715 nm with an intensity that is linearly dependent on the excitation power. We attribute this shorter wavelength emission to anti-Stokes fluorescence. Similar anti-Stokes fluorescence was also detected focally in all skin-derived samples containing porphyrins. Regular (Stokes) fluorescence was present under UV and visible light excitation on ex vivo nasal skin and sebum from uninflamed acne, but not on nose surface smears or sebum from inflamed acne. Co-registered CW laser-excited anti-Stokes fluorescence and fs laser-excited multi-photon fluorescence images of PpIX powder showed similar features. In the skin samples because of the anti-Stokes effect, the NIR-induced fluorescence was presumably specific for porphyrins since there appeared to be no anti-Stokes emission signals from other typical skin fluorophores such as lipids, keratins and collagen. Anti-Stokes fluorescence under NIR CW excitation is more sensitive and specific for porphyrin detection than UV- or visible light-excited regular fluorescence and fs laser-excited multi-photon fluorescence. This approach also has higher image contrast compared to NIR fs laser-based multi-photon fluorescence imaging. The anti-Stokes fluorescence of porphyrins within sebum could potentially be applied to detecting and targeting acne lesions for treatment via fluorescence image guidance.

Stimulated Raman photoacoustic imaging

PubMed Central

Yakovlev, Vladislav V.; Zhang, Hao F.; Noojin, Gary D.; Denton, Michael L.; Thomas, Robert J.; Scully, Marlan O.

2010-01-01

Achieving label-free, molecular-specific imaging with high spatial resolution in deep tissue is often considered the grand challenge of optical imaging. To accomplish this goal, significant optical scattering in tissues has to be overcome while achieving molecular specificity without resorting to extrinsic labeling. We demonstrate the feasibility of developing such an optical imaging modality by combining the molecularly specific stimulated Raman excitation with the photoacoustic detection. By employing two ultrashort excitation laser pulses, separated in frequency by the vibrational frequency of a targeted molecule, only the specific vibrational level of the target molecules in the illuminated tissue volume is excited. This targeted optical absorption generates ultrasonic waves (referred to as stimulated Raman photoacoustic waves) which are detected using a traditional ultrasonic transducer to form an image following the design of the established photoacoustic microscopy. PMID:21059930

Using axicons for depth discrimination in excitation-emission laser scanning imaging systems

NASA Astrophysics Data System (ADS)

Iglesias, Ignacio

2017-10-01

Besides generating good approximations to zero-order Bessel beams, an axicon lens coupled to a spatial filter can be used to collect light while preserving information on the depth coordinate of the source location. To demonstrate the principle, we describe an experimental excitation-emission fluorescence imaging system that uses an axicon twice: to generate an excitation Bessel beam and to collect the emitted light.

Two-photon speckle illumination for super-resolution microscopy.

PubMed

Negash, Awoke; Labouesse, Simon; Chaumet, Patrick C; Belkebir, Kamal; Giovannini, Hugues; Allain, Marc; Idier, Jérôme; Sentenac, Anne

2018-06-01

We present a numerical study of a microscopy setup in which the sample is illuminated with uncontrolled speckle patterns and the two-photon excitation fluorescence is collected on a camera. We show that, using a simple deconvolution algorithm for processing the speckle low-resolution images, this wide-field imaging technique exhibits resolution significantly better than that of two-photon excitation scanning microscopy or one-photon excitation bright-field microscopy.

Pseudo color ghost coding imaging with pseudo thermal light

NASA Astrophysics Data System (ADS)

Duan, De-yang; Xia, Yun-jie

2018-04-01

We present a new pseudo color imaging scheme named pseudo color ghost coding imaging based on ghost imaging but with multiwavelength source modulated by a spatial light modulator. Compared with conventional pseudo color imaging where there is no nondegenerate wavelength spatial correlations resulting in extra monochromatic images, the degenerate wavelength and nondegenerate wavelength spatial correlations between the idle beam and signal beam can be obtained simultaneously. This scheme can obtain more colorful image with higher quality than that in conventional pseudo color coding techniques. More importantly, a significant advantage of the scheme compared to the conventional pseudo color coding imaging techniques is the image with different colors can be obtained without changing the light source and spatial filter.

Imaging of Subsurface Corrosion Using Gradient-Field Pulsed Eddy Current Probes with Uniform Field Excitation

PubMed Central

Ren, Shuting; Yan, Bei; Zainal Abidin, Ilham Mukriz; Wang, Yi

2017-01-01

A corrosive environment leaves in-service conductive structures prone to subsurface corrosion which poses a severe threat to the structural integrity. It is indispensable to detect and quantitatively evaluate subsurface corrosion via non-destructive evaluation techniques. Although the gradient-field pulsed eddy current technique (GPEC) has been found to be superior in the evaluation of corrosion in conductors, it suffers from a technical drawback resulting from the non-uniform field excited by the conventional pancake coil. In light of this, a new GPEC probe with uniform field excitation for the imaging of subsurface corrosion is proposed in this paper. The excited uniform field makes the GPEC signal correspond only to the field perturbation due to the presence of subsurface corrosion, which benefits the corrosion profiling and sizing. A 3D analytical model of GPEC is established to analyze the characteristics of the uniform field induced within a conductor. Following this, experiments regarding the imaging of subsurface corrosion via GPEC have been carried out. It has been found from the results that the proposed GPEC probe with uniform field excitation not only applies to the imaging of subsurface corrosion in conductive structures, but provides high-sensitivity imaging results regarding the corrosion profile and opening size. PMID:28758985

Imaging of Subsurface Corrosion Using Gradient-Field Pulsed Eddy Current Probes with Uniform Field Excitation.

PubMed

Li, Yong; Ren, Shuting; Yan, Bei; Zainal Abidin, Ilham Mukriz; Wang, Yi

2017-07-31

A corrosive environment leaves in-service conductive structures prone to subsurface corrosion which poses a severe threat to the structural integrity. It is indispensable to detect and quantitatively evaluate subsurface corrosion via non-destructive evaluation techniques. Although the gradient-field pulsed eddy current technique (GPEC) has been found to be superior in the evaluation of corrosion in conductors, it suffers from a technical drawback resulting from the non-uniform field excited by the conventional pancake coil. In light of this, a new GPEC probe with uniform field excitation for the imaging of subsurface corrosion is proposed in this paper. The excited uniform field makes the GPEC signal correspond only to the field perturbation due to the presence of subsurface corrosion, which benefits the corrosion profiling and sizing. A 3D analytical model of GPEC is established to analyze the characteristics of the uniform field induced within a conductor. Following this, experiments regarding the imaging of subsurface corrosion via GPEC have been carried out. It has been found from the results that the proposed GPEC probe with uniform field excitation not only applies to the imaging of subsurface corrosion in conductive structures, but provides high-sensitivity imaging results regarding the corrosion profile and opening size.

Entanglement negativity and sudden death in the toric code at finite temperature

NASA Astrophysics Data System (ADS)

Hart, O.; Castelnovo, C.

2018-04-01

We study the fate of quantum correlations at finite temperature in the two-dimensional toric code using the logarithmic entanglement negativity. We are able to obtain exact results that give us insight into how thermal excitations affect quantum entanglement. The toric code has two types of elementary excitations (defects) costing different energies. We show that an O (1 ) density of the lower energy defect is required to degrade the zero-temperature entanglement between two subsystems in contact with one another. However, one type of excitation alone is not sufficient to kill all quantum correlations, and an O (1 ) density of the higher energy defect is required to cause the so-called sudden death of the negativity. Interestingly, if the energy cost of one of the excitations is taken to infinity, quantum correlations survive up to arbitrarily high temperatures, a feature that is likely shared with other quantum spin liquids and frustrated systems in general, when projected down to their low-energy states. We demonstrate this behavior both for small subsystems, where we can prove that the negativity is a necessary and sufficient condition for separability, as well as for extended subsystems, where it is only a necessary condition. We further observe that the negativity per boundary degree of freedom at a given temperature increases (parametrically) with the size of the boundary, and that quantum correlations between subsystems with extended boundaries are more robust to thermal fluctuations.

Locality-preserving logical operators in topological stabilizer codes

NASA Astrophysics Data System (ADS)

Webster, Paul; Bartlett, Stephen D.

2018-01-01

Locality-preserving logical operators in topological codes are naturally fault tolerant, since they preserve the correctability of local errors. Using a correspondence between such operators and gapped domain walls, we describe a procedure for finding all locality-preserving logical operators admitted by a large and important class of topological stabilizer codes. In particular, we focus on those equivalent to a stack of a finite number of surface codes of any spatial dimension, where our procedure fully specifies the group of locality-preserving logical operators. We also present examples of how our procedure applies to codes with different boundary conditions, including color codes and toric codes, as well as more general codes such as Abelian quantum double models and codes with fermionic excitations in more than two dimensions.

Optimized atom position and coefficient coding for matching pursuit-based image compression.

PubMed

Shoa, Alireza; Shirani, Shahram

2009-12-01

In this paper, we propose a new encoding algorithm for matching pursuit image coding. We show that coding performance is improved when correlations between atom positions and atom coefficients are both used in encoding. We find the optimum tradeoff between efficient atom position coding and efficient atom coefficient coding and optimize the encoder parameters. Our proposed algorithm outperforms the existing coding algorithms designed for matching pursuit image coding. Additionally, we show that our algorithm results in better rate distortion performance than JPEG 2000 at low bit rates.

Parallel-multiplexed excitation light-sheet microscopy (Conference Presentation)

NASA Astrophysics Data System (ADS)

Xu, Dongli; Zhou, Weibin; Peng, Leilei

2017-02-01

Laser scanning light-sheet imaging allows fast 3D image of live samples with minimal bleach and photo-toxicity. Existing light-sheet techniques have very limited capability in multi-label imaging. Hyper-spectral imaging is needed to unmix commonly used fluorescent proteins with large spectral overlaps. However, the challenge is how to perform hyper-spectral imaging without sacrificing the image speed, so that dynamic and complex events can be captured live. We report wavelength-encoded structured illumination light sheet imaging (λ-SIM light-sheet), a novel light-sheet technique that is capable of parallel multiplexing in multiple excitation-emission spectral channels. λ-SIM light-sheet captures images of all possible excitation-emission channels in true parallel. It does not require compromising the imaging speed and is capable of distinguish labels by both excitation and emission spectral properties, which facilitates unmixing fluorescent labels with overlapping spectral peaks and will allow more labels being used together. We build a hyper-spectral light-sheet microscope that combined λ-SIM with an extended field of view through Bessel beam illumination. The system has a 250-micron-wide field of view and confocal level resolution. The microscope, equipped with multiple laser lines and an unlimited number of spectral channels, can potentially image up to 6 commonly used fluorescent proteins from blue to red. Results from in vivo imaging of live zebrafish embryos expressing various genetic markers and sensors will be shown. Hyper-spectral images from λ-SIM light-sheet will allow multiplexed and dynamic functional imaging in live tissue and animals.

Optimizing ultrafast illumination for multiphoton-excited fluorescence imaging

PubMed Central

Stoltzfus, Caleb R.; Rebane, Aleksander

2016-01-01

We study the optimal conditions for high throughput two-photon excited fluorescence (2PEF) and three-photon excited fluorescence (3PEF) imaging using femtosecond lasers. We derive relations that allow maximization of the rate of imaging depending on the average power, pulse repetition rate, and noise characteristics of the laser, as well as on the size and structure of the sample. We perform our analysis using ~100 MHz, ~1 MHz and 1 kHz pulse rates and using both a tightly-focused illumination beam with diffraction-limited image resolution, as well loosely focused illumination with a relatively low image resolution, where the latter utilizes separate illumination and fluorescence detection beam paths. Our theoretical estimates agree with the experiments, which makes our approach especially useful for optimizing high throughput imaging of large samples with a field-of-view up to 10x10 cm2. PMID:27231620

Unique identification code for medical fundus images using blood vessel pattern for tele-ophthalmology applications.

PubMed

Singh, Anushikha; Dutta, Malay Kishore; Sharma, Dilip Kumar

2016-10-01

Identification of fundus images during transmission and storage in database for tele-ophthalmology applications is an important issue in modern era. The proposed work presents a novel accurate method for generation of unique identification code for identification of fundus images for tele-ophthalmology applications and storage in databases. Unlike existing methods of steganography and watermarking, this method does not tamper the medical image as nothing is embedded in this approach and there is no loss of medical information. Strategic combination of unique blood vessel pattern and patient ID is considered for generation of unique identification code for the digital fundus images. Segmented blood vessel pattern near the optic disc is strategically combined with patient ID for generation of a unique identification code for the image. The proposed method of medical image identification is tested on the publically available DRIVE and MESSIDOR database of fundus image and results are encouraging. Experimental results indicate the uniqueness of identification code and lossless recovery of patient identity from unique identification code for integrity verification of fundus images. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Color-coded fluid-attenuated inversion recovery images improve inter-rater reliability of fluid-attenuated inversion recovery signal changes within acute diffusion-weighted image lesions.

PubMed

Kim, Bum Joon; Kim, Yong-Hwan; Kim, Yeon-Jung; Ahn, Sung Ho; Lee, Deok Hee; Kwon, Sun U; Kim, Sang Joon; Kim, Jong S; Kang, Dong-Wha

2014-09-01

Diffusion-weighted image fluid-attenuated inversion recovery (FLAIR) mismatch has been considered to represent ischemic lesion age. However, the inter-rater agreement of diffusion-weighted image FLAIR mismatch is low. We hypothesized that color-coded images would increase its inter-rater agreement. Patients with ischemic stroke <24 hours of a clear onset were retrospectively studied. FLAIR signal change was rated as negative, subtle, or obvious on conventional and color-coded FLAIR images based on visual inspection. Inter-rater agreement was evaluated using κ and percent agreement. The predictive value of diffusion-weighted image FLAIR mismatch for identification of patients <4.5 hours of symptom onset was evaluated. One hundred and thirteen patients were enrolled. The inter-rater agreement of FLAIR signal change improved from 69.9% (k=0.538) with conventional images to 85.8% (k=0.754) with color-coded images (P=0.004). Discrepantly rated patients on conventional, but not on color-coded images, had a higher prevalence of cardioembolic stroke (P=0.02) and cortical infarction (P=0.04). The positive predictive value for patients <4.5 hours of onset was 85.3% and 71.9% with conventional and 95.7% and 82.1% with color-coded images, by each rater. Color-coded FLAIR images increased the inter-rater agreement of diffusion-weighted image FLAIR recovery mismatch and may ultimately help identify unknown-onset stroke patients appropriate for thrombolysis. © 2014 American Heart Association, Inc.

Wavelet-based compression of M-FISH images.

PubMed

Hua, Jianping; Xiong, Zixiang; Wu, Qiang; Castleman, Kenneth R

2005-05-01

Multiplex fluorescence in situ hybridization (M-FISH) is a recently developed technology that enables multi-color chromosome karyotyping for molecular cytogenetic analysis. Each M-FISH image set consists of a number of aligned images of the same chromosome specimen captured at different optical wavelength. This paper presents embedded M-FISH image coding (EMIC), where the foreground objects/chromosomes and the background objects/images are coded separately. We first apply critically sampled integer wavelet transforms to both the foreground and the background. We then use object-based bit-plane coding to compress each object and generate separate embedded bitstreams that allow continuous lossy-to-lossless compression of the foreground and the background. For efficient arithmetic coding of bit planes, we propose a method of designing an optimal context model that specifically exploits the statistical characteristics of M-FISH images in the wavelet domain. Our experiments show that EMIC achieves nearly twice as much compression as Lempel-Ziv-Welch coding. EMIC also performs much better than JPEG-LS and JPEG-2000 for lossless coding. The lossy performance of EMIC is significantly better than that of coding each M-FISH image with JPEG-2000.

Integrated femtosecond stimulated Raman scattering and two-photon fluorescence imaging of subcellular lipid and vesicular structures

NASA Astrophysics Data System (ADS)

Li, Xuesong; Lam, Wen Jiun; Cao, Zhe; Hao, Yan; Sun, Qiqi; He, Sicong; Mak, Ho Yi; Qu, Jianan Y.

2015-11-01

The primary goal of this study is to demonstrate that stimulated Raman scattering (SRS) as a new imaging modality can be integrated into a femtosecond (fs) nonlinear optical (NLO) microscope system. The fs sources of high pulse peak power are routinely used in multimodal nonlinear microscopy to enable efficient excitation of multiple NLO signals. However, with fs excitations, the SRS imaging of subcellular lipid and vesicular structures encounters significant interference from proteins due to poor spectral resolution and a lack of chemical specificity, respectively. We developed a unique NLO microscope of fs excitation that enables rapid acquisition of SRS and multiple two-photon excited fluorescence (TPEF) signals. In the in vivo imaging of transgenic C. elegans animals, we discovered that by cross-filtering false positive lipid signals based on the TPEF signals from tryptophan-bearing endogenous proteins and lysosome-related organelles, the imaging system produced highly accurate assignment of SRS signals to lipid. Furthermore, we demonstrated that the multimodal NLO microscope system could sequentially image lipid structure/content and organelles, such as mitochondria, lysosomes, and the endoplasmic reticulum, which are intricately linked to lipid metabolism.

Infrared imaging results of an excited planar jet

DOE Office of Scientific and Technical Information (OSTI.GOV)

Farrington, R.B.

1991-12-01

Planar jets are used for many applications including heating, cooling, and ventilation. Generally such a jet is designed to provide good mixing within an enclosure. In building applications, the jet provides both thermal comfort and adequate indoor air quality. Increased mixing rates may lead to lower short-circuiting of conditioned air, elimination of dead zones within the occupied zone, reduced energy costs, increased occupant comfort, and higher indoor air quality. This paper discusses using an infrared imaging system to show the effect of excitation of a jet on the spread angle and on the jet mixing efficiency. Infrared imaging captures amore » large number of data points in real time (over 50,000 data points per image) providing significant advantages over single-point measurements. We used a screen mesh with a time constant of approximately 0.3 seconds as a target for the infrared camera to detect temperature variations in the jet. The infrared images show increased jet spread due to excitation of the jet. Digital data reduction and analysis show change in jet isotherms and quantify the increased mixing caused by excitation. 17 refs., 20 figs.« less

Separating Bulk and Surface Contributions to Electronic Excited-State Processes in Hybrid Mixed Perovskite Thin Films via Multimodal All-Optical Imaging

DOE PAGES

Simpson, Mary Jane; Doughty, Benjamin; Das, Sanjib; ...

2017-07-04

A comprehensive understanding of electronic excited-state phenomena underlying the impressive performance of solution-processed hybrid halide perovskite solar cells requires access to both spatially resolved electronic processes and corresponding sample morphological characteristics. In this paper, we demonstrate an all-optical multimodal imaging approach that enables us to obtain both electronic excited-state and morphological information on a single optical microscope platform with simultaneous high temporal and spatial resolution. Specifically, images were acquired for the same region of interest in thin films of chloride containing mixed lead halide perovskites (CH 3NH 3PbI 3–xCl x) using femtosecond transient absorption, time-integrated photoluminescence, confocal reflectance, and transmissionmore » microscopies. Comprehensive image analysis revealed the presence of surface- and bulk-dominated contributions to the various images, which describe either spatially dependent electronic excited-state properties or morphological variations across the probed region of the thin films. Finally, these results show that PL probes effectively the species near or at the film surface.« less

Hadamard-Encoded Multipulses for Contrast-Enhanced Ultrasound Imaging.

PubMed

Gong, Ping; Song, Pengfei; Chen, Shigao

2017-11-01

The development of contrast-enhanced ultrasound (CEUS) imaging offers great opportunities for new ultrasound clinical applications such as myocardial perfusion imaging and abdominal lesion characterization. In CEUS imaging, the contrast agents (i.e., microbubbles) are utilized to improve the contrast between blood and tissue based on their high nonlinearity under low ultrasound pressure. In this paper, we propose a new CEUS pulse sequence by combining Hadamard-encoded multipulses (HEM) with fundamental frequency bandpass filter (i.e., filter centered on transmit frequency). HEM consecutively emits multipulses encoded by a second-order Hadamard matrix in each of the two transmission events (i.e., pulse-echo events), as opposed to conventional CEUS methods which emit individual pulses in two separate transmission events (i.e., pulse inversion (PI), amplitude modulation (AM), and PIAM). In HEM imaging, the microbubble responses can be improved by the longer transmit pulse, and the tissue harmonics can be suppressed by the fundamental frequency filter, leading to significantly improved contrast-to-tissue ratio (CTR) and signal-to-noise ratio (SNR). In addition, the fast polarity change between consecutive coded pulse emissions excites strong nonlinear microbubble echoes, further enhancing the CEUS image quality. The spatial resolution of HEM image is compromised as compared to other microbubble imaging methods due to the longer transmit pulses and the lower imaging frequency (i.e., fundamental frequency). However, the resolution loss was shown to be negligible and could be offset by the significantly enhanced CTR, SNR, and penetration depth. These properties of HEM can potentially facilitate robust CEUS imaging for many clinical applications, especially for deep abdominal organs and heart.

Lossless compression of VLSI layout image data.

PubMed

Dai, Vito; Zakhor, Avideh

2006-09-01

We present a novel lossless compression algorithm called Context Copy Combinatorial Code (C4), which integrates the advantages of two very disparate compression techniques: context-based modeling and Lempel-Ziv (LZ) style copying. While the algorithm can be applied to many lossless compression applications, such as document image compression, our primary target application has been lossless compression of integrated circuit layout image data. These images contain a heterogeneous mix of data: dense repetitive data better suited to LZ-style coding, and less dense structured data, better suited to context-based encoding. As part of C4, we have developed a novel binary entropy coding technique called combinatorial coding which is simultaneously as efficient as arithmetic coding, and as fast as Huffman coding. Compression results show C4 outperforms JBIG, ZIP, BZIP2, and two-dimensional LZ, and achieves lossless compression ratios greater than 22 for binary layout image data, and greater than 14 for gray-pixel image data.

A job for quantum dots: use of a smartphone and 3D-printed accessory for all-in-one excitation and imaging of photoluminescence.

PubMed

Petryayeva, Eleonora; Algar, W Russ

2016-04-01

Point-of-care (POC) diagnostic technologies are needed to improve global health and smartphones are a prospective platform for these technologies. While many fluorescence or photoluminescence-based smartphone assays have been reported in the literature, common shortcomings are the requirement of an excitation light source external to the smartphone and complicated integration of that excitation source with the smartphone. Here, we show that the photographic flash associated with the smartphone camera can be utilized to enable all-in-one excitation and imaging of photoluminescence (PL), thus eliminating the need for an excitation light source external to the smartphone. A simple and low-cost 3D-printed accessory was designed to create a dark environment and direct excitation light from the smartphone flash onto a sample. Multiple colors and compositions of semiconductor quantum dot (QD) were evaluated as photoluminescent materials for all-in-one smartphone excitation and imaging of PL, and these were compared with fluorescein and R-phycoerythrin (R-PE), which are widely utilized molecular and protein materials for fluorescence-based bioanalysis. The QDs were found to exhibit much better brightness and have the best potential for two-color detection. A model protein binding assay with a sub-microgram per milliliter detection limit and a Förster resonance energy transfer (FRET) assay for proteolytic activity were demonstrated, including imaging with serum as a sample matrix. In addition, FRET within tandem conjugates of a QD donor and fluorescent dye acceptor enabled smartphone detection of dye fluorescence that was otherwise unobservable without the QD to enhance its brightness. The ideal properties of photoluminescent materials for all-in-one smartphone excitation and imaging are discussed in the context of several different materials, where QDs appear to be the best overall material for this application.

Binary image encryption in a joint transform correlator scheme by aid of run-length encoding and QR code

NASA Astrophysics Data System (ADS)

Qin, Yi; Wang, Zhipeng; Wang, Hongjuan; Gong, Qiong

2018-07-01

We propose a binary image encryption method in joint transform correlator (JTC) by aid of the run-length encoding (RLE) and Quick Response (QR) code, which enables lossless retrieval of the primary image. The binary image is encoded with RLE to obtain the highly compressed data, and then the compressed binary image is further scrambled using a chaos-based method. The compressed and scrambled binary image is then transformed into one QR code that will be finally encrypted in JTC. The proposed method successfully, for the first time to our best knowledge, encodes a binary image into a QR code with the identical size of it, and therefore may probe a new way for extending the application of QR code in optical security. Moreover, the preprocessing operations, including RLE, chaos scrambling and the QR code translation, append an additional security level on JTC. We present digital results that confirm our approach.

Evaluation of excitation strategy with multi-plane electrical capacitance tomography sensor

NASA Astrophysics Data System (ADS)

Mao, Mingxu; Ye, Jiamin; Wang, Haigang; Zhang, Jiaolong; Yang, Wuqiang

2016-11-01

Electrical capacitance tomography (ECT) is an imaging technique for measuring the permittivity change of materials. Using a multi-plane ECT sensor, three-dimensional (3D) distribution of permittivity may be represented. In this paper, three excitation strategies, including single-electrode excitation, dual-electrode excitation in the same plane, and dual-electrode excitation in different planes are investigated by numerical simulation and experiment for two three-plane ECT sensors with 12 electrodes in total. In one sensor, the electrodes on the middle plane are in line with the others. In the other sensor, they are rotated 45° with reference to the other two planes. A linear back projection algorithm is used to reconstruct the images and a correlation coefficient is used to evaluate the image quality. The capacitance data and sensitivity distribution with each measurement strategy and sensor model are analyzed. Based on simulation and experimental results using noise-free and noisy capacitance data, the performance of the three strategies is evaluated.

Combining image-processing and image compression schemes

NASA Technical Reports Server (NTRS)

Greenspan, H.; Lee, M.-C.

1995-01-01

An investigation into the combining of image-processing schemes, specifically an image enhancement scheme, with existing compression schemes is discussed. Results are presented on the pyramid coding scheme, the subband coding scheme, and progressive transmission. Encouraging results are demonstrated for the combination of image enhancement and pyramid image coding schemes, especially at low bit rates. Adding the enhancement scheme to progressive image transmission allows enhanced visual perception at low resolutions. In addition, further progressing of the transmitted images, such as edge detection schemes, can gain from the added image resolution via the enhancement.

Selective visualization of fluorescent sterols in Caenorhabditis elegans by bleach-rate-based image segmentation.

PubMed

Wüstner, Daniel; Landt Larsen, Ane; Faergeman, Nils J; Brewer, Jonathan R; Sage, Daniel

2010-04-01

The nematode Caenorhabditis elegans is a genetically tractable model organism to investigate sterol transport. In vivo imaging of the fluorescent sterol, dehydroergosterol (DHE), is challenged by C. elegans' high autofluorescence in the same spectral region as emission of DHE. We present a method to detect DHE selectively, based on its rapid bleaching kinetics compared to cellular autofluorescence. Worms were repeatedly imaged on an ultraviolet-sensitive wide field (UV-WF) microscope, and bleaching kinetics of DHE were fitted on a pixel-basis to mathematical models describing the intensity decay. Bleach-rate constants were determined for DHE in vivo and confirmed in model membranes. Using this method, we could detect enrichment of DHE in specific tissues like the nerve ring, the spermateca and oocytes. We confirm these results in C. elegans gut-granule-loss (glo) mutants with reduced autofluorescence and compare our method with three-photon excitation microscopy of sterol in selected tissues. Bleach-rate-based UV-WF imaging is a useful tool for genetic screening experiments on sterol transport, as exemplified by RNA interference against the rme-2 gene coding for the yolk receptor and for worm homologues of Niemann-Pick C disease proteins. Our approach is generally useful for identifying fluorescent probes in the presence of high cellular autofluorescence.

CMOS Imaging of Pin-Printed Xerogel-Based Luminescent Sensor Microarrays.

PubMed

Yao, Lei; Yung, Ka Yi; Khan, Rifat; Chodavarapu, Vamsy P; Bright, Frank V

2010-12-01

We present the design and implementation of a luminescence-based miniaturized multisensor system using pin-printed xerogel materials which act as host media for chemical recognition elements. We developed a CMOS imager integrated circuit (IC) to image the luminescence response of the xerogel-based sensor array. The imager IC uses a 26 × 20 (520 elements) array of active pixel sensors and each active pixel includes a high-gain phototransistor to convert the detected optical signals into electrical currents. The imager includes a correlated double sampling circuit and pixel address/digital control circuit; the image data is read-out as coded serial signal. The sensor system uses a light-emitting diode (LED) to excite the target analyte responsive luminophores doped within discrete xerogel-based sensor elements. As a prototype, we developed a 4 × 4 (16 elements) array of oxygen (O 2 ) sensors. Each group of 4 sensor elements in the array (arranged in a row) is designed to provide a different and specific sensitivity to the target gaseous O 2 concentration. This property of multiple sensitivities is achieved by using a strategic mix of two oxygen sensitive luminophores ([Ru(dpp) 3 ] 2+ and ([Ru(bpy) 3 ] 2+ ) in each pin-printed xerogel sensor element. The CMOS imager consumes an average power of 8 mW operating at 1 kHz sampling frequency driven at 5 V. The developed prototype system demonstrates a low cost and miniaturized luminescence multisensor system.

Simulated imaging properties of a series of magnetic electron lenses

NASA Technical Reports Server (NTRS)

Kory, Carol L.

1995-01-01

The paraxial lens data were determined for a series of symmetrical magnetic lenses of equal lens diameter but variable air gap width for a wide range of lens excitations using the three-dimensional electrodynamic computer code MAFIA. The results are compared with a similar study done by Liebman and Grad wherein the field distributions within the lenses were measured experimentally with a resistance network analogue. Using these fields the lens data were obtained through numerical trajectory tracing. The utility of using MAFIA, instead of experimental methods for lens design is shown by the excellent agreement of the simulated results compared to experiment. Also demonstrated is the capability of using MAFIA to investigate aberration sources such as higher order off-axis magnetic field and space-charge effects.

Three-dimensional integral imaging displays using a quick-response encoded elemental image array: an overview

NASA Astrophysics Data System (ADS)

Markman, A.; Javidi, B.

2016-06-01

Quick-response (QR) codes are barcodes that can store information such as numeric data and hyperlinks. The QR code can be scanned using a QR code reader, such as those built into smartphone devices, revealing the information stored in the code. Moreover, the QR code is robust to noise, rotation, and illumination when scanning due to error correction built in the QR code design. Integral imaging is an imaging technique used to generate a three-dimensional (3D) scene by combining the information from two-dimensional (2D) elemental images (EIs) each with a different perspective of a scene. Transferring these 2D images in a secure manner can be difficult. In this work, we overview two methods to store and encrypt EIs in multiple QR codes. The first method uses run-length encoding with Huffman coding and the double-random-phase encryption (DRPE) to compress and encrypt an EI. This information is then stored in a QR code. An alternative compression scheme is to perform photon-counting on the EI prior to compression. Photon-counting is a non-linear transformation of data that creates redundant information thus improving image compression. The compressed data is encrypted using the DRPE. Once information is stored in the QR codes, it is scanned using a smartphone device. The information scanned is decompressed and decrypted and an EI is recovered. Once all EIs have been recovered, a 3D optical reconstruction is generated.

UV fluorescence excitation imaging of healing of wounds in skin: Evaluation of wound closure in organ culture model.

PubMed

Wang, Ying; Gutierrez-Herrera, Enoch; Ortega-Martinez, Antonio; Anderson, Richard Rox; Franco, Walfre

2016-09-01

Molecules native to tissue that fluoresce upon light excitation can serve as reporters of cellular activity and protein structure. In skin, the fluorescence ascribed to tryptophan is a marker of cellular proliferation, whereas the fluorescence ascribed to cross-links of collagen is a structural marker. In this work, we introduce and demonstrate a simple but robust optical method to image the functional process of epithelialization and the exposed dermal collagen in wound healing of human skin in an organ culture model. Non-closing non-grafted, partial closing non-grafted, and grafted wounds were created in ex vivo human skin and kept in culture. A wide-field UV fluorescence excitation imaging system was used to visualize epithelialization of the exposed dermis and quantitate wound area, closure, and gap. Histology (H&E staining) was also used to evaluate epithelialization. The endogenous fluorescence excitation of cross-links of collagen at 335 nm clearly shows the dermis missing epithelium, while the endogenous fluorescence excitation of tryptophan at 295 nm shows keratinocytes in higher proliferating state. The size of the non-closing wound was 11.4 ± 1.8 mm and remained constant during the observation period, while the partial-close wound reached 65.5 ± 4.9% closure by day 16. Evaluations of wound gaps using fluorescence excitation images and histology images are in agreement. We have established a fluorescence imaging method for studying epithelialization processes, evaluating keratinocyte proliferation, and quantitating closure during wound healing of skin in an organ culture model: the dermal fluorescence of pepsin-digestible collagen cross-links can be used to quantitate wound size, closure extents, and gaps; and, the epidermal fluorescence ascribed to tryptophan can be used to monitor and quantitate functional states of epithelialization. UV fluorescence excitation imaging has the potential to become a valuable tool for research, diagnostic and educational purposes on evaluating the healing of wounds. Lasers Surg. Med. 48:678-685, 2016. © 2016 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals, Inc. © 2016 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals, Inc.

Holographic Photolysis for Multiple Cell Stimulation in Mouse Hippocampal Slices

PubMed Central

Papagiakoumou, Eirini; Ventalon, Cathie; Angulo, María Cecilia; Emiliani, Valentina

2010-01-01

Background Advanced light microscopy offers sensitive and non-invasive means to image neural activity and to control signaling with photolysable molecules and, recently, light-gated channels. These approaches require precise and yet flexible light excitation patterns. For synchronous stimulation of subsets of cells, they also require large excitation areas with millisecond and micrometric resolution. We have recently developed a new method for such optical control using a phase holographic modulation of optical wave-fronts, which minimizes power loss, enables rapid switching between excitation patterns, and allows a true 3D sculpting of the excitation volumes. In previous studies we have used holographic photololysis to control glutamate uncaging on single neuronal cells. Here, we extend the use of holographic photolysis for the excitation of multiple neurons and of glial cells. Methods/Principal Findings The system combines a liquid crystal device for holographic patterned photostimulation, high-resolution optical imaging, the HiLo microscopy, to define the stimulated regions and a conventional Ca2+ imaging system to detect neural activity. By means of electrophysiological recordings and calcium imaging in acute hippocampal slices, we show that the use of excitation patterns precisely tailored to the shape of multiple neuronal somata represents a very efficient way for the simultaneous excitation of a group of neurons. In addition, we demonstrate that fast shaped illumination patterns also induce reliable responses in single glial cells. Conclusions/Significance We show that the main advantage of holographic illumination is that it allows for an efficient excitation of multiple cells with a spatiotemporal resolution unachievable with other existing approaches. Although this paper focuses on the photoactivation of caged molecules, our approach will surely prove very efficient for other probes, such as light-gated channels, genetically encoded photoactivatable proteins, photoactivatable fluorescent proteins, and voltage-sensitive dyes. PMID:20195547

ALCBEAM - Neutral beam formation and propagation code for beam-based plasma diagnostics

NASA Astrophysics Data System (ADS)

Bespamyatnov, I. O.; Rowan, W. L.; Liao, K. T.

2012-03-01

ALCBEAM is a new three-dimensional neutral beam formation and propagation code. It was developed to support the beam-based diagnostics installed on the Alcator C-Mod tokamak. The purpose of the code is to provide reliable estimates of the local beam equilibrium parameters: such as beam energy fractions, density profiles and excitation populations. The code effectively unifies the ion beam formation, extraction and neutralization processes with beam attenuation and excitation in plasma and neutral gas and beam stopping by the beam apertures. This paper describes the physical processes interpreted and utilized by the code, along with exploited computational methods. The description is concluded by an example simulation of beam penetration into plasma of Alcator C-Mod. The code is successfully being used in Alcator C-Mod tokamak and expected to be valuable in the support of beam-based diagnostics in most other tokamak environments. Program summaryProgram title: ALCBEAM Catalogue identifier: AEKU_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEKU_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 66 459 No. of bytes in distributed program, including test data, etc.: 7 841 051 Distribution format: tar.gz Programming language: IDL Computer: Workstation, PC Operating system: Linux RAM: 1 GB Classification: 19.2 Nature of problem: Neutral beams are commonly used to heat and/or diagnose high-temperature magnetically-confined laboratory plasmas. An accurate neutral beam characterization is required for beam-based measurements of plasma properties. Beam parameters such as density distribution, energy composition, and atomic excited populations of the beam atoms need to be known. Solution method: A neutral beam is initially formed as an ion beam which is extracted from the ion source by high voltage applied to the extraction and accelerating grids. The current distribution of a single beamlet emitted from a single pore of IOS depends on the shape of the plasma boundary in the emission region. Total beam extracted by IOS is calculated at every point of 3D mesh as sum of all contributions from each grid pore. The code effectively unifies the ion beam formation, extraction and neutralization processes with neutral beam attenuation and excitation in plasma and neutral gas and beam stopping by the beam apertures. Running time: 10 min for a standard run.

Fully phase-encoded MRI near metallic implants using ultrashort echo times and broadband excitation.

PubMed

Wiens, Curtis N; Artz, Nathan S; Jang, Hyungseok; McMillan, Alan B; Koch, Kevin M; Reeder, Scott B

2018-04-01

To develop a fully phase-encoded MRI method for distortion-free imaging near metallic implants, in clinically feasible acquisition times. An accelerated 3D fully phase-encoded acquisition with broadband excitation and ultrashort echo times is presented, which uses a broadband radiofrequency pulse to excite the entire off-resonance induced by the metallic implant. Furthermore, fully phase-encoded imaging is used to prevent distortions caused by frequency encoding, and to obtain ultrashort echo times for rapidly decaying signal. Phantom and in vivo acquisitions were used to describe the relationship among excitation bandwidth, signal loss near metallic implants, and T 1 weighting. Shorter radiofrequency pulses captured signal closer to the implant by improving spectral coverage and allowing shorter echo times, whereas longer pulses improved T 1 weighting through larger maximum attainable flip angles. Comparisons of fully phase-encoded acquisition with broadband excitation and ultrashort echo times to T 1 -weighted multi-acquisition with variable resonance image combination selective were performed in phantoms and subjects with metallic knee and hip prostheses. These acquisitions had similar contrast and acquisition efficiency. Accelerated fully phase-encoded acquisitions with ultrashort echo times and broadband excitation can generate distortion free images near metallic implants in clinically feasible acquisition times. Magn Reson Med 79:2156-2163, 2018. © 2017 International Society for Magnetic Resonance in Medicine. © 2017 International Society for Magnetic Resonance in Medicine.

Fully Phase-Encoded MRI Near Metallic Implants Using Ultrashort Echo Times and Broadband Excitation

PubMed Central

Wiens, Curtis N.; Artz, Nathan S.; Jang, Hyungseok; McMillan, Alan B.; Koch, Kevin M.; Reeder, Scott B.

2017-01-01

Purpose To develop a fully phase-encoded MRI method for distortion-free imaging near metallic implants, in clinically feasible acquisition times. Theory and Methods An accelerated 3D fully phase-encoded acquisition with broadband excitation and ultrashort echo times is presented, which uses a broadband radiofrequency pulse to excite the entire off-resonance induced by the metallic implant. Furthermore, fully phase-encoded imaging is used to prevent distortions caused by frequency encoding, and to obtain ultrashort echo times for rapidly decaying signal. Results Phantom and in vivo acquisitions were used to describe the relationship among excitation bandwidth, signal loss near metallic implants, and T1 weighting. Shorter radiofrequency pulses captured signal closer to the implant by improving spectral coverage and allowing shorter echo times, whereas longer pulses improved T1 weighting through larger maximum attainable flip angles. Comparisons of fully phase-encoded acquisition with broadband excitation and ultrashort echo times to T1-weighted multi-acquisition with variable resonance image combination selective were performed in phantoms and subjects with metallic knee and hip prostheses. These acquisitions had similar contrast and acquisition efficiency. Conclusions Accelerated fully phase-encoded acquisitions with ultrashort echo times and broadband excitation can generate distortion free images near metallic implants in clinically feasible acquisition times. Magn Reson Med 000:000–000, 2017. PMID:28833407

Comparison of active-set method deconvolution and matched-filtering for derivation of an ultrasound transit time spectrum.

PubMed

Wille, M-L; Zapf, M; Ruiter, N V; Gemmeke, H; Langton, C M

2015-06-21

The quality of ultrasound computed tomography imaging is primarily determined by the accuracy of ultrasound transit time measurement. A major problem in analysis is the overlap of signals making it difficult to detect the correct transit time. The current standard is to apply a matched-filtering approach to the input and output signals. This study compares the matched-filtering technique with active set deconvolution to derive a transit time spectrum from a coded excitation chirp signal and the measured output signal. The ultrasound wave travels in a direct and a reflected path to the receiver, resulting in an overlap in the recorded output signal. The matched-filtering and deconvolution techniques were applied to determine the transit times associated with the two signal paths. Both techniques were able to detect the two different transit times; while matched-filtering has a better accuracy (0.13 μs versus 0.18 μs standard deviations), deconvolution has a 3.5 times improved side-lobe to main-lobe ratio. A higher side-lobe suppression is important to further improve image fidelity. These results suggest that a future combination of both techniques would provide improved signal detection and hence improved image fidelity.

Synaptic E-I Balance Underlies Efficient Neural Coding.

PubMed

Zhou, Shanglin; Yu, Yuguo

2018-01-01

Both theoretical and experimental evidence indicate that synaptic excitation and inhibition in the cerebral cortex are well-balanced during the resting state and sensory processing. Here, we briefly summarize the evidence for how neural circuits are adjusted to achieve this balance. Then, we discuss how such excitatory and inhibitory balance shapes stimulus representation and information propagation, two basic functions of neural coding. We also point out the benefit of adopting such a balance during neural coding. We conclude that excitatory and inhibitory balance may be a fundamental mechanism underlying efficient coding.

Synaptic E-I Balance Underlies Efficient Neural Coding

PubMed Central

Zhou, Shanglin; Yu, Yuguo

2018-01-01

Both theoretical and experimental evidence indicate that synaptic excitation and inhibition in the cerebral cortex are well-balanced during the resting state and sensory processing. Here, we briefly summarize the evidence for how neural circuits are adjusted to achieve this balance. Then, we discuss how such excitatory and inhibitory balance shapes stimulus representation and information propagation, two basic functions of neural coding. We also point out the benefit of adopting such a balance during neural coding. We conclude that excitatory and inhibitory balance may be a fundamental mechanism underlying efficient coding. PMID:29456491

Optimal optical filters of fluorescence excitation and emission for poultry fecal detection

USDA-ARS?s Scientific Manuscript database

Purpose: An analytic method to design excitation and emission filters of a multispectral fluorescence imaging system is proposed and was demonstrated in an application to poultry fecal inspection. Methods: A mathematical model of a multispectral imaging system is proposed and its system parameters, ...

A fractional Fourier transform analysis of the scattering of ultrasonic waves.

PubMed

Tant, Katherine M M; Mulholland, Anthony J; Langer, Matthias; Gachagan, Anthony

2015-03-08

Many safety critical structures, such as those found in nuclear plants, oil pipelines and in the aerospace industry, rely on key components that are constructed from heterogeneous materials. Ultrasonic non-destructive testing (NDT) uses high-frequency mechanical waves to inspect these parts, ensuring they operate reliably without compromising their integrity. It is possible to employ mathematical models to develop a deeper understanding of the acquired ultrasonic data and enhance defect imaging algorithms. In this paper, a model for the scattering of ultrasonic waves by a crack is derived in the time-frequency domain. The fractional Fourier transform (FrFT) is applied to an inhomogeneous wave equation where the forcing function is prescribed as a linear chirp, modulated by a Gaussian envelope. The homogeneous solution is found via the Born approximation which encapsulates information regarding the flaw geometry. The inhomogeneous solution is obtained via the inverse Fourier transform of a Gaussian-windowed linear chirp excitation. It is observed that, although the scattering profile of the flaw does not change, it is amplified. Thus, the theory demonstrates the enhanced signal-to-noise ratio permitted by the use of coded excitation, as well as establishing a time-frequency domain framework to assist in flaw identification and classification.

Pyramid image codes

NASA Technical Reports Server (NTRS)

Watson, Andrew B.

1990-01-01

All vision systems, both human and machine, transform the spatial image into a coded representation. Particular codes may be optimized for efficiency or to extract useful image features. Researchers explored image codes based on primary visual cortex in man and other primates. Understanding these codes will advance the art in image coding, autonomous vision, and computational human factors. In cortex, imagery is coded by features that vary in size, orientation, and position. Researchers have devised a mathematical model of this transformation, called the Hexagonal oriented Orthogonal quadrature Pyramid (HOP). In a pyramid code, features are segregated by size into layers, with fewer features in the layers devoted to large features. Pyramid schemes provide scale invariance, and are useful for coarse-to-fine searching and for progressive transmission of images. The HOP Pyramid is novel in three respects: (1) it uses a hexagonal pixel lattice, (2) it uses oriented features, and (3) it accurately models most of the prominent aspects of primary visual cortex. The transform uses seven basic features (kernels), which may be regarded as three oriented edges, three oriented bars, and one non-oriented blob. Application of these kernels to non-overlapping seven-pixel neighborhoods yields six oriented, high-pass pyramid layers, and one low-pass (blob) layer.

Multiphoton Rydberg and valence dynamics of CH3Br probed by mass spectrometry and slice imaging.

PubMed

Hafliðason, Arnar; Glodic, Pavle; Koumarianou, Greta; Samartzis, Peter C; Kvaran, Ágúst

2018-06-18

The multiphoton dynamics of CH3Br were probed by Mass Resolved MultiPhoton Ionization (MR-MPI), Slice Imaging and Photoelectron Imaging in the two-photon excitation region of 66 000 to 80 000 cm-1. Slice images of the CH3+ and Br+ photoproducts of ten two-photon resonant transitions to np and nd Rydberg states of the parent molecule were recorded. CH3+ ions dominate the mass spectra. Kinetic energy release spectra (KERs) were derived from slice and photoelectron images and anisotropy parameters were extracted from the angular distributions of the ions to identify the processes and the dynamics involved. At all wavelengths we observe three-photon excitations, via the two-photon resonant transitions to molecular Rydberg states, forming metastable, superexcited (CH3Br#) states which dissociate to form CH3 Rydberg states (CH3**) along with Br/Br*. A correlation between the parent Rydberg states excited and CH3** formed is evident. For the three highest excitation energies used, the CH3Br# metastable states also generate high kinetic energy fragments of CH3(X) and Br/Br*. In addition for two out of these three wavelengths we also measure one-photon photolysis of CH3Br in the A band forming CH3(X) in various vibrational modes and bromine atoms in the ground (Br) and spin-orbit excited (Br*) states.

Signature of chaos in the 4 f -core-excited states for highly-charged tungsten ions

NASA Astrophysics Data System (ADS)

Safronova, Ulyana; Safronova, Alla

2014-05-01

We evaluate radiative and autoionizing transition rates in highly charged W ions in search for the signature of chaos. In particularly, previously published results for Ag-like W27+, Tm-like W5+, and Yb-like W4+ ions as well as newly obtained for I-like W21+, Xe-like W20+, Cs-like W19+, and La-like W17+ ions (with ground configuration [Kr] 4d10 4fk with k = 7, 8, 9, and 11, respectively) are considered that were calculated using the multiconfiguration relativistic Hebrew University Lawrence Livermore Atomic Code (HULLAC code) and the Hartree-Fock-Relativistic method (COWAN code). The main emphasis was on verification of Gaussian statistics of rates as a function of transition energy. There was no evidence of such statistics for above mentioned previously published results as well as for the transitions between the excited and autoionizing states for newly calculated results. However, we did find the Gaussian profile for the transitions between excited states such as the [Kr] 4d10 4fk - [Kr] 4d10 4f k - 1 5 d transitions , for newly calculated W ions. This work is supported in part by DOE under NNSA Cooperative Agreement DE-NA0001984.

Validation of the Electromagnetic Code FACETS for Numerical Simulation of Radar Target Images

DTIC Science & Technology

2009-12-01

Validation of the electromagnetic code FACETS for numerical simulation of radar target images S. Wong...Validation of the electromagnetic code FACETS for numerical simulation of radar target images S. Wong DRDC Ottawa...for simulating radar images of a target is obtained, through direct simulation-to-measurement comparisons. A 3-dimensional computer-aided design

Optical image encryption based on real-valued coding and subtracting with the help of QR code

NASA Astrophysics Data System (ADS)

Deng, Xiaopeng

2015-08-01

A novel optical image encryption based on real-valued coding and subtracting is proposed with the help of quick response (QR) code. In the encryption process, the original image to be encoded is firstly transformed into the corresponding QR code, and then the corresponding QR code is encoded into two phase-only masks (POMs) by using basic vector operations. Finally, the absolute values of the real or imaginary parts of the two POMs are chosen as the ciphertexts. In decryption process, the QR code can be approximately restored by recording the intensity of the subtraction between the ciphertexts, and hence the original image can be retrieved without any quality loss by scanning the restored QR code with a smartphone. Simulation results and actual smartphone collected results show that the method is feasible and has strong tolerance to noise, phase difference and ratio between intensities of the two decryption light beams.

Side information in coded aperture compressive spectral imaging

NASA Astrophysics Data System (ADS)

Galvis, Laura; Arguello, Henry; Lau, Daniel; Arce, Gonzalo R.

2017-02-01

Coded aperture compressive spectral imagers sense a three-dimensional cube by using two-dimensional projections of the coded and spectrally dispersed source. These imagers systems often rely on FPA detectors, SLMs, micromirror devices (DMDs), and dispersive elements. The use of the DMDs to implement the coded apertures facilitates the capture of multiple projections, each admitting a different coded aperture pattern. The DMD allows not only to collect the sufficient number of measurements for spectrally rich scenes or very detailed spatial scenes but to design the spatial structure of the coded apertures to maximize the information content on the compressive measurements. Although sparsity is the only signal characteristic usually assumed for reconstruction in compressing sensing, other forms of prior information such as side information have been included as a way to improve the quality of the reconstructions. This paper presents the coded aperture design in a compressive spectral imager with side information in the form of RGB images of the scene. The use of RGB images as side information of the compressive sensing architecture has two main advantages: the RGB is not only used to improve the reconstruction quality but to optimally design the coded apertures for the sensing process. The coded aperture design is based on the RGB scene and thus the coded aperture structure exploits key features such as scene edges. Real reconstructions of noisy compressed measurements demonstrate the benefit of the designed coded apertures in addition to the improvement in the reconstruction quality obtained by the use of side information.

A novel three-dimensional image reconstruction method for near-field coded aperture single photon emission computerized tomography

PubMed Central

Mu, Zhiping; Hong, Baoming; Li, Shimin; Liu, Yi-Hwa

2009-01-01

Coded aperture imaging for two-dimensional (2D) planar objects has been investigated extensively in the past, whereas little success has been achieved in imaging 3D objects using this technique. In this article, the authors present a novel method of 3D single photon emission computerized tomography (SPECT) reconstruction for near-field coded aperture imaging. Multiangular coded aperture projections are acquired and a stack of 2D images is reconstructed separately from each of the projections. Secondary projections are subsequently generated from the reconstructed image stacks based on the geometry of parallel-hole collimation and the variable magnification of near-field coded aperture imaging. Sinograms of cross-sectional slices of 3D objects are assembled from the secondary projections, and the ordered subset expectation and maximization algorithm is employed to reconstruct the cross-sectional image slices from the sinograms. Experiments were conducted using a customized capillary tube phantom and a micro hot rod phantom. Imaged at approximately 50 cm from the detector, hot rods in the phantom with diameters as small as 2.4 mm could be discerned in the reconstructed SPECT images. These results have demonstrated the feasibility of the authors’ 3D coded aperture image reconstruction algorithm for SPECT, representing an important step in their effort to develop a high sensitivity and high resolution SPECT imaging system. PMID:19544769

Imaging charge transfer in a cation-π system: velocity-map imaging of Ag(+)(benzene) photodissociation.

PubMed

Maner, Jonathon A; Mauney, Daniel T; Duncan, Michael A

2015-11-19

Ag(+)(benzene) complexes are generated in the gas phase by laser vaporization and mass selected in a time-of-flight spectrometer. UV laser excitation at either 355 or 266 nm results in dissociative charge transfer (DCT), leading to neutral silver atom and benzene cation products. Kinetic energy release in translationally hot benzene cations is detected using a new instrument designed for photofragment imaging of mass-selected ions. Velocity-map imaging and slice imaging techniques are employed. In addition to the expected translational energy release, DCT of Ag(+)(benzene) produces a distribution of internally hot benzene cations. Compared with experiments at 355 nm, 266 nm excitation produces only slightly higher translational excitation and a much greater fraction of internally hot benzene ions. The maximum kinetic energy release in the photodissociation sets an upper limit on the Ag(+)(benzene) dissociation energy of 32.8 (+1.4/-1.5) kcal/mol.

The design of wavefront coded imaging system

NASA Astrophysics Data System (ADS)

Lan, Shun; Cen, Zhaofeng; Li, Xiaotong

2016-10-01

Wavefront Coding is a new method to extend the depth of field, which combines optical design and signal processing together. By using optical design software ZEMAX ,we designed a practical wavefront coded imaging system based on a conventional Cooke triplet system .Unlike conventional optical system, the wavefront of this new system is modulated by a specially designed phase mask, which makes the point spread function (PSF)of optical system not sensitive to defocus. Therefore, a series of same blurred images obtained at the image plane. In addition, the optical transfer function (OTF) of the wavefront coded imaging system is independent of focus, which is nearly constant with misfocus and has no regions of zeros. All object information can be completely recovered through digital filtering at different defocus positions. The focus invariance of MTF is selected as merit function in this design. And the coefficients of phase mask are set as optimization goals. Compared to conventional optical system, wavefront coded imaging system obtains better quality images under different object distances. Some deficiencies appear in the restored images due to the influence of digital filtering algorithm, which are also analyzed in this paper. The depth of field of the designed wavefront coded imaging system is about 28 times larger than initial optical system, while keeping higher optical power and resolution at the image plane.

A novel small molecule mediate 18F-FDG excited fluorescence molecular imaging

NASA Astrophysics Data System (ADS)

Zhang, Zeyu; Guo, Hongbo; Hu, Zhenhua; Tian, Jie

2018-02-01

Fluorescence molecular imaging (FMI) has been widely used in many medical fields with small molecule indocyanine green (ICG). However, low signal-background ratio and limited specificity to tumor remain big challenges for FMI. In this study, a novel excitation strategy is proposed on the basis of clinical approved ICG and 18F-FDG. A series of in vitro experiments are designed to reveal the mechanism and results show obvious decreasing of ICG fluorescence intensity with the increasing distance to excitation source. Meanwhile, the ICG fluorescence intensity is proportional to the activity of radiopharmaceutical. Results from different respects illustrate the promising of this proposed excitation strategy.

Remote excitation and detection of surface-enhanced Raman scattering from graphene.

PubMed

Coca-López, Nicolás; Hartmann, Nicolai F; Mancabelli, Tobia; Kraus, Jürgen; Günther, Sebastian; Comin, Alberto; Hartschuh, Achim

2018-06-07

We demonstrate the remote excitation and detection of surface-enhanced Raman scattering (SERS) from graphene using a silver nanowire as a plasmonic waveguide. By investigating a nanowire touching a graphene sheet at only one terminal, we first show the remote excitation of SERS from graphene by propagating surface plasmon polaritons (SPPs) launched by a focused laser over distances on the order of 10 μm. Remote detection of SERS is then demonstrated for the same nanowire by detecting light emission at the distal end of the nanowire that was launched by graphene Raman scattering and carried to the end of the nanowire by SPPs. We then show that the transfer of the excitation and Raman scattered light along the nanowire can also be visualized through spectrally selective back focal plane imaging. Back focal plane images detected upon focused laser excitation at one of the nanowire's tips reveal propagating surface plasmon polaritons at the laser energy and at the energies of the most prominent Raman bands of graphene. With this approach the identification of remote excitation and detection of SERS for nanowires completely covering the Raman scatterer is achieved, which is typically not possible by direct imaging.

The Role of Inhibition in a Computational Model of an Auditory Cortical Neuron during the Encoding of Temporal Information

PubMed Central

Bendor, Daniel

2015-01-01

In auditory cortex, temporal information within a sound is represented by two complementary neural codes: a temporal representation based on stimulus-locked firing and a rate representation, where discharge rate co-varies with the timing between acoustic events but lacks a stimulus-synchronized response. Using a computational neuronal model, we find that stimulus-locked responses are generated when sound-evoked excitation is combined with strong, delayed inhibition. In contrast to this, a non-synchronized rate representation is generated when the net excitation evoked by the sound is weak, which occurs when excitation is coincident and balanced with inhibition. Using single-unit recordings from awake marmosets (Callithrix jacchus), we validate several model predictions, including differences in the temporal fidelity, discharge rates and temporal dynamics of stimulus-evoked responses between neurons with rate and temporal representations. Together these data suggest that feedforward inhibition provides a parsimonious explanation of the neural coding dichotomy observed in auditory cortex. PMID:25879843

^235U(n,xnγ) Excitation Function Measurements Using Gamma-Ray Spectroscopy at GEANIE

NASA Astrophysics Data System (ADS)

Younes, W.; Becker, J. A.; Bernstein, L. A.; Archer, D. E.; Stoyer, M. A.; Hauschild, K.; Drake, D. M.; Johns, G. D.; Nelson, R. O.; Wilburn, S. W.

1998-04-01

The ^235U(n,xn) cross sections (where x<=2) have previously been measured at several incident neutron energies. In particular, the ^235U(n,2n) cross section has been measured(J. Frehaut et al.), Nucl. Sci. Eng. 74,29 (1980). reliably up to peak near E_n≈ 11 MeV, but not along the tail which is predicted by some(M.B. Chadwick, private communication.) codes to yield significant (e.g. >= 10% of peak) cross section out to E_n≈ 30 MeV. We have measured gamma-ray spectra resulting from ^235U(n,xn) as a function of neutron energy in the range 1 MeV <~ En <~ 200 MeV using the GEANIE spectrometer at the LANSCE/WNR ``white'' neutron source. We will present excitation functions for the de-excitation gamma rays in ^234,235U compared to predictions from the Hauser-Feshbach-preequilibrium code GNASH(M.B. Chadwick and P.G. Young, Los Alamos Report No. LA-UR-93-104, 1993.).

Start-Up Scenario in Gyrotrons with a Nonstationary Microwave-Field Structure

NASA Astrophysics Data System (ADS)

Nusinovich, G. S.; Yeddulla, M.; Antonsen, T. M., Jr.; Vlasov, A. N.

2006-03-01

Megawatt class gyrotrons operate in very high-order modes. Therefore, control of a gyrotron oscillator’s start-up is important for excitation of the desired mode in the presence of the many undesired modes. Analysis of such scenario using the self-consistent code MAGY [M. Botton , IEEE Trans. Plasma Sci. 26,ITPSBD0093-3813 882 (1998)10.1109/27.700860] reveals that during start-up not only mode amplitudes vary in time, but also their axial structure can be time dependent. Simulations done for a 1.5 MW gyrotron show that the excitation of a single operating TE22,6 mode can exhibit a sort of intermittency when, first, it is excited as a mode whose axial structure extends outside the interaction cavity, then it ceases and then reappears as a mode mostly localized in the cavity. This phenomenon makes it necessary to analyze start-up scenarios in such gyrotrons with the use of codes that account for the possible evolution of field profiles.

Infrared Photothermal Radiometry.

DTIC Science & Technology

1984-04-10

changes whenever the transmitted thermal wave crosses a void. This provides a means of nondestructive subsurface imaging of defects, and Busse found that...15 In the flash excitation, the excitation beam is modulated by a broad spectrum of Fourier modulation frequencies. In all cases of subsurface imaging , the...technique of Nordal and Kanstad 2 1t 23 is not only good for spectroscopic detection, but also for subsurface imaging applications as well. 2.4 Pulsed

Phase synchronization motion and neural coding in dynamic transmission of neural information.

PubMed

Wang, Rubin; Zhang, Zhikang; Qu, Jingyi; Cao, Jianting

2011-07-01

In order to explore the dynamic characteristics of neural coding in the transmission of neural information in the brain, a model of neural network consisting of three neuronal populations is proposed in this paper using the theory of stochastic phase dynamics. Based on the model established, the neural phase synchronization motion and neural coding under spontaneous activity and stimulation are examined, for the case of varying network structure. Our analysis shows that, under the condition of spontaneous activity, the characteristics of phase neural coding are unrelated to the number of neurons participated in neural firing within the neuronal populations. The result of numerical simulation supports the existence of sparse coding within the brain, and verifies the crucial importance of the magnitudes of the coupling coefficients in neural information processing as well as the completely different information processing capability of neural information transmission in both serial and parallel couplings. The result also testifies that under external stimulation, the bigger the number of neurons in a neuronal population, the more the stimulation influences the phase synchronization motion and neural coding evolution in other neuronal populations. We verify numerically the experimental result in neurobiology that the reduction of the coupling coefficient between neuronal populations implies the enhancement of lateral inhibition function in neural networks, with the enhancement equivalent to depressing neuronal excitability threshold. Thus, the neuronal populations tend to have a stronger reaction under the same stimulation, and more neurons get excited, leading to more neurons participating in neural coding and phase synchronization motion.

Quadrature transmit coil for breast imaging at 7 tesla using forced current excitation for improved homogeneity.

PubMed

McDougall, Mary Preston; Cheshkov, Sergey; Rispoli, Joseph; Malloy, Craig; Dimitrov, Ivan; Wright, Steven M

2014-11-01

To demonstrate the use of forced current excitation (FCE) to create homogeneous excitation of the breast at 7 tesla, insensitive to the effects of asymmetries in the electrical environment. FCE was implemented on two breast coils: one for quadrature (1) H imaging and one for proton-decoupled (13) C spectroscopy. Both were a Helmholtz-saddle combination, with the saddle tuned to 298 MHz for imaging and 75 MHz for spectroscopy. Bench measurements were acquired to demonstrate the ability to force equal currents on elements in the presence of asymmetric loading to improve homogeneity. Modeling and temperature measurements were conducted per safety protocol. B1 mapping, imaging, and proton-decoupled (13) C spectroscopy were demonstrated in vivo. Using FCE to ensure balanced currents on elements enabled straightforward tuning and maintaining of isolation between quadrature elements of the coil. Modeling and bench measurements confirmed homogeneity of the field, which resulted in images with excellent fat suppression and in broadband proton-decoupled carbon-13 spectra. FCE is a straightforward approach to ensure equal currents on multiple coil elements and a homogeneous excitation field, insensitive to the effects of asymmetries in the electrical environment. This enabled effective breast imaging and proton-decoupled carbon-13 spectroscopy at 7T. © 2014 Wiley Periodicals, Inc.

Laser line illumination scheme allowing the reduction of background signal and the correction of absorption heterogeneities effects for fluorescence reflectance imaging.

PubMed

Fantoni, Frédéric; Hervé, Lionel; Poher, Vincent; Gioux, Sylvain; Mars, Jérôme I; Dinten, Jean-Marc

2015-10-01

Intraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality as it allows to noninvasively monitor the fluorescence targeted tumors located below the tissue surface. Some drawbacks of this technique are the background fluorescence decreasing the contrast and absorption heterogeneities leading to misinterpretations concerning fluorescence concentrations. We propose a correction technique based on a laser line scanning illumination scheme. We scan the medium with the laser line and acquire, at each position of the line, both fluorescence and excitation images. We then use the finding that there is a relationship between the excitation intensity profile and the background fluorescence one to predict the amount of signal to subtract from the fluorescence images to get a better contrast. As the light absorption information is contained both in fluorescence and excitation images, this method also permits us to correct the effects of absorption heterogeneities. This technique has been validated on simulations and experimentally. Fluorescent inclusions are observed in several configurations at depths ranging from 1 mm to 1 cm. Results obtained with this technique are compared with those obtained with a classical wide-field detection scheme for contrast enhancement and with the fluorescence by an excitation ratio approach for absorption correction.

Real-time computer treatment of THz passive device images with the high image quality

NASA Astrophysics Data System (ADS)

Trofimov, Vyacheslav A.; Trofimov, Vladislav V.

2012-06-01

We demonstrate real-time computer code improving significantly the quality of images captured by the passive THz imaging system. The code is not only designed for a THz passive device: it can be applied to any kind of such devices and active THz imaging systems as well. We applied our code for computer processing of images captured by four passive THz imaging devices manufactured by different companies. It should be stressed that computer processing of images produced by different companies requires using the different spatial filters usually. The performance of current version of the computer code is greater than one image per second for a THz image having more than 5000 pixels and 24 bit number representation. Processing of THz single image produces about 20 images simultaneously corresponding to various spatial filters. The computer code allows increasing the number of pixels for processed images without noticeable reduction of image quality. The performance of the computer code can be increased many times using parallel algorithms for processing the image. We develop original spatial filters which allow one to see objects with sizes less than 2 cm. The imagery is produced by passive THz imaging devices which captured the images of objects hidden under opaque clothes. For images with high noise we develop an approach which results in suppression of the noise after using the computer processing and we obtain the good quality image. With the aim of illustrating the efficiency of the developed approach we demonstrate the detection of the liquid explosive, ordinary explosive, knife, pistol, metal plate, CD, ceramics, chocolate and other objects hidden under opaque clothes. The results demonstrate the high efficiency of our approach for the detection of hidden objects and they are a very promising solution for the security problem.

A Spherical Active Coded Aperture for 4π Gamma-ray Imaging

DOE PAGES

Hellfeld, Daniel; Barton, Paul; Gunter, Donald; ...

2017-09-22

Gamma-ray imaging facilitates the efficient detection, characterization, and localization of compact radioactive sources in cluttered environments. Fieldable detector systems employing active planar coded apertures have demonstrated broad energy sensitivity via both coded aperture and Compton imaging modalities. But, planar configurations suffer from a limited field-of-view, especially in the coded aperture mode. In order to improve upon this limitation, we introduce a novel design by rearranging the detectors into an active coded spherical configuration, resulting in a 4pi isotropic field-of-view for both coded aperture and Compton imaging. This work focuses on the low- energy coded aperture modality and the optimization techniquesmore » used to determine the optimal number and configuration of 1 cm 3 CdZnTe coplanar grid detectors on a 14 cm diameter sphere with 192 available detector locations.« less

QR code based noise-free optical encryption and decryption of a gray scale image

NASA Astrophysics Data System (ADS)

Jiao, Shuming; Zou, Wenbin; Li, Xia

2017-03-01

In optical encryption systems, speckle noise is one major challenge in obtaining high quality decrypted images. This problem can be addressed by employing a QR code based noise-free scheme. Previous works have been conducted for optically encrypting a few characters or a short expression employing QR codes. This paper proposes a practical scheme for optically encrypting and decrypting a gray-scale image based on QR codes for the first time. The proposed scheme is compatible with common QR code generators and readers. Numerical simulation results reveal the proposed method can encrypt and decrypt an input image correctly.

Multiphoton versus confocal high resolution z-sectioning of enhanced green fluorescent microtubules: increased multiphoton photobleaching within the focal plane can be compensated using a Pockels cell and dual widefield detectors.

PubMed

Drummond, D R; Carter, N; Cross, R A

2002-05-01

Multiphoton excitation was originally projected to improve live cell fluorescence imaging by minimizing photobleaching effects outside the focal plane, yet reports suggest that photobleaching within the focal plane is actually worse than with one photon excitation. We confirm that when imaging enhanced green fluorescent protein, photobleaching is indeed more acute within the multiphoton excitation volume, so that whilst fluorescence increases as predicted with the square of the excitation power, photobleaching rates increase with a higher order relationship. Crucially however, multiphoton excitation also affords unique opportunities for substantial improvements to fluorescence detection. By using a Pockels cell to minimize exposure of the specimen together with multiple nondescanned detectors we show quantitatively that for any particular bleach rate multiphoton excitation produces significantly more signal than one photon excitation confocal microscopy in high resolution Z-axis sectioning of thin samples. Both modifications are readily implemented on a commercial multiphoton microscope system.

Self-referenced axial chromatic dispersion measurement in multiphoton microscopy through 2-color THG imaging.

PubMed

Du, Yu; Zhuang, Ziwei; He, Jiexing; Liu, Hongji; Qiu, Ping; Wang, Ke

2018-05-16

With tunable excitation light, multiphoton microscopy (MPM) is widely used for imaging biological structures at subcellular resolution. Axial chromatic dispersion, present in virtually every transmissive optical system including the multiphoton microscope, leads to focal (and the resultant image) plane separation. Here we demonstrate experimentally a technique to measure the axial chromatic dispersion in a multiphoton microscope, using simultaneous 2-color third-harmonic generation (THG) imaging excited by a 2-color soliton source with tunable wavelength separation. Our technique is self-referenced, eliminating potential measurement error when 1-color tunable excitation light is used which necessitates reciprocating motion of the mechanical translation stage. Using this technique, we demonstrate measured axial chromatic dispersion with 2 different objective lenses in a multiphoton microscope. Further measurement in a biological sample also indicates that this axial chromatic dispersion, in combination with 2-color imaging, may open up opportunity for simultaneous imaging of two different axial planes. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

In-vivo multi-nonlinear optical imaging of a living cell using a supercontinuum light source generated from a photonic crystal fiber

NASA Astrophysics Data System (ADS)

Kano, Hideaki; Hamaguchi, Hiro-O.

2006-04-01

A supercontinuum light source generated with a femtosecond Ti:Sapphire oscillator has been used to obtain both vibrational and two-photon excitation fluorescence (TPEF) images of a living cell simultaneously at different wavelengths. Owing to an ultrabroadband spectral profile of the supercontinuum, multiple vibrational resonances have been detected through coherent anti-Stokes Raman scattering (CARS) process. In addition to the multiplex CARS process, multiple electronic states can be excited due to the broadband electronic two-photon excitation using the supercontinuum, giving rise to a two-photon excitation fluorescence (TPEF) signal. Using a living yeast cell whose nucleus is labeled by green fluorescent protein (GFP), we have succeeded in visualizing organelles such as mitochondria, septum, and nucleus through the CARS and the TPEF processes. The supercontinuum enables us to perform unique multi-nonlinear optical imaging through two different nonlinear optical processes.

Coded mask telescopes for X-ray astronomy

NASA Astrophysics Data System (ADS)

Skinner, G. K.; Ponman, T. J.

1987-04-01

The principle of the coded mask techniques are discussed together with the methods of image reconstruction. The coded mask telescopes built at the University of Birmingham, including the SL 1501 coded mask X-ray telescope flown on the Skylark rocket and the Coded Mask Imaging Spectrometer (COMIS) projected for the Soviet space station Mir, are described. A diagram of a coded mask telescope and some designs for coded masks are included.

High compression image and image sequence coding

NASA Technical Reports Server (NTRS)

Kunt, Murat

1989-01-01

The digital representation of an image requires a very large number of bits. This number is even larger for an image sequence. The goal of image coding is to reduce this number, as much as possible, and reconstruct a faithful duplicate of the original picture or image sequence. Early efforts in image coding, solely guided by information theory, led to a plethora of methods. The compression ratio reached a plateau around 10:1 a couple of years ago. Recent progress in the study of the brain mechanism of vision and scene analysis has opened new vistas in picture coding. Directional sensitivity of the neurones in the visual pathway combined with the separate processing of contours and textures has led to a new class of coding methods capable of achieving compression ratios as high as 100:1 for images and around 300:1 for image sequences. Recent progress on some of the main avenues of object-based methods is presented. These second generation techniques make use of contour-texture modeling, new results in neurophysiology and psychophysics and scene analysis.

Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging.

PubMed

Zhao, Ming; Li, Yu; Peng, Leilei

2014-05-05

We present a novel excitation-emission multiplexed fluorescence lifetime microscopy (FLIM) method that surpasses current FLIM techniques in multiplexing capability. The method employs Fourier multiplexing to simultaneously acquire confocal fluorescence lifetime images of multiple excitation wavelength and emission color combinations at 44,000 pixels/sec. The system is built with low-cost CW laser sources and standard PMTs with versatile spectral configuration, which can be implemented as an add-on to commercial confocal microscopes. The Fourier lifetime confocal method allows fast multiplexed FLIM imaging, which makes it possible to monitor multiple biological processes in live cells. The low cost and compatibility with commercial systems could also make multiplexed FLIM more accessible to biological research community.

Cardiovascular Imaging Using Two-Photon Microscopy

PubMed Central

Scherschel, John A.; Rubart, Michael

2008-01-01

Two-photon excitation microscopy has become the standard technique for high resolution deep tissue and intravital imaging. It provides intrinsic three-dimensional resolution in combination with increased penetration depth compared to single-photon confocal microscopy. This article will describe the basic physical principles of two-photon excitation and will review its multiple applications to cardiovascular imaging, including second harmonic generation and fluorescence laser scanning microscopy. In particular, the capability and limitations of multiphoton microscopy to assess functional heterogeneity on a cellular scale deep within intact, Langendorff-perfused hearts are demonstrated. It will also discuss the use of two-photon excitation-induced release of caged compounds for the study of intracellular calcium signaling and intercellular dye transfer. PMID:18986603

CMOS Imaging of Temperature Effects on Pin-Printed Xerogel Sensor Microarrays.

PubMed

Lei Yao; Ka Yi Yung; Chodavarapu, Vamsy P; Bright, Frank V

2011-04-01

In this paper, we study the effect of temperature on the operation and performance of a xerogel-based sensor microarrays coupled to a complementary metal-oxide semiconductor (CMOS) imager integrated circuit (IC) that images the photoluminescence response from the sensor microarray. The CMOS imager uses a 32 × 32 (1024 elements) array of active pixel sensors and each pixel includes a high-gain phototransistor to convert the detected optical signals into electrical currents. A correlated double sampling circuit and pixel address/digital control/signal integration circuit are also implemented on-chip. The CMOS imager data are read out as a serial coded signal. The sensor system uses a light-emitting diode to excite target analyte responsive organometallic luminophores doped within discrete xerogel-based sensor elements. As a proto type, we developed a 3 × 3 (9 elements) array of oxygen (O2) sensors. Each group of three sensor elements in the array (arranged in a column) is designed to provide a different and specific sensitivity to the target gaseous O2 concentration. This property of multiple sensitivities is achieved by using a mix of two O2 sensitive luminophores in each pin-printed xerogel sensor element. The CMOS imager is designed to be low noise and consumes a static power of 320.4 μW and an average dynamic power of 624.6 μW when operating at 100-Hz sampling frequency and 1.8-V dc power supply.

Imaging Magnetospheric Boundries at Ionospheric Heights

NASA Astrophysics Data System (ADS)

Baumgardner, J.; Nottingham, D.; Wroten, J.; Mendillo, M.

2001-12-01

Stable auroral red (SAR) arcs are excited by a downward heat flux within a narrow range of fluxtubes that define the plasmapause-ring current interaction region. Ambient F-region electrons near and above the peak height (300-500 km) are heated and collisionally excite atomic oxygen to the O(1D) state, thereby emitting 6300 A photons. At the same time, the diffuse aurora at 6300 A is excited by the precipitation of plasma sheet electrons into the lower thermosphere, exciting O(1D) to emit near 200 km. An all-sky imaging system operating at a sub-auroral site (e.g., at Millstone Hill) can readily record the SAR arc centroid location and the equatorial edge of the diffuse aurora in the same 6300 A image. We have analyzed 75 such cases showing where both stuctures occur in the ionosphere and then conducted field-line mapping to define the L-shell domains of origin in the equatorial plane of the inner magnetosphere (L ~ 2.5 - 4). To within the measurement and mapping accuracies, both boundaries coincide, i.e., the inner edge of the plasma sheet essentially falls along the plasmapause. Since the O(1D) 6300 A emission corresponds to ~2 ev of excitation by magnetospheric processes, this technique defines ELENA (Extremely Low Energetic Neutral Atom) imaging of magnetospheric structures.

Multi-Excitation Magnetoacoustic Tomography with Magnetic Induction for Bioimpedance Imaging

PubMed Central

Li, Xu; He, Bin

2011-01-01

Magnetoacoustic tomography with magnetic induction (MAT-MI) is an imaging approach proposed to conduct non-invasive electrical conductivity imaging of biological tissue with high spatial resolution. In the present study, based on the analysis of the relationship between the conductivity distribution and the generated MAT-MI acoustic source, we propose a new multi-excitation MAT-MI approach and the corresponding reconstruction algorithms. In the proposed method, multiple magnetic excitations using different coil configurations are employed and ultrasound measurements corresponding to each excitation are collected to derive the conductivity distribution inside the sample. A modified reconstruction algorithm is also proposed for the multi-excitation MAT-MI imaging approach when only limited bandwidth acoustic measurements are available. Computer simulation and phantom experiment studies have been done to demonstrate the merits of the proposed method. It is shown that if unlimited bandwidth acoustic data is available, we can accurately reconstruct the internal conductivity contrast of an object using the proposed method. With limited bandwidth data and the use of the modified algorithm we can reconstruct the relative conductivity contrast of an object instead of only boundaries at the conductivity heterogeneity. Benefits that come with this new method include better differentiation of tissue types with conductivity contrast using the MAT-MI approach, specifically for potential breast cancer screening application in the future. PMID:20529729

Temporal evolution of atmosphere pressure plasma jets driven by microsecond pulses with positive and negative polarities

NASA Astrophysics Data System (ADS)

Shao, Tao; Yang, Wenjin; Zhang, Cheng; Fang, Zhi; Zhou, Yixiao; Schamiloglu, Edl

2014-09-01

Current-voltage characteristics, discharge images, and optical spectra of atmospheric pressure plasma jets (APPJs) are studied using a microsecond pulse length generator producing repetitive output pulses with different polarities. The experimental results show that the APPJs excited by the pulses with positive polarity have longer plume, faster propagation speed, higher power, and more excited species, such as \\text{N}2 , O, He, \\text{N}2+ , than that with the negatively excited APPJs. The images taken using an intensified charge-coupled device show that the APPJs excited by pulses with positive polarity are characterized by a bullet-like structure, while the APPJs excited by pulses with negative polarity are continuous. The propagation speed of the APPJs driven by a microsecond pulse length generator is about tens of km/s, which is similar to the APPJs driven by a kHz frequency sinusoidal voltage source. The analysis shows that the space charge accumulation effect plays an important role during the discharge. The transient enhanced electric field induced by the accumulated ions between the needle-like electrode and the nozzle in the APPJs excited by pulses with negative polarity enhances electron field emission from the cathode, which is illustrated by the bright line on the time-integrated images. This makes the shape of the APPJ excited using pulses with negative polarity different from the bullet-like shape of the APPJs excited by pulses with positive polarity.

Cone-beam x-ray luminescence computed tomography based on x-ray absorption dosage

NASA Astrophysics Data System (ADS)

Liu, Tianshuai; Rong, Junyan; Gao, Peng; Zhang, Wenli; Liu, Wenlei; Zhang, Yuanke; Lu, Hongbing

2018-02-01

With the advances of x-ray excitable nanophosphors, x-ray luminescence computed tomography (XLCT) has become a promising hybrid imaging modality. In particular, a cone-beam XLCT (CB-XLCT) system has demonstrated its potential in in vivo imaging with the advantage of fast imaging speed over other XLCT systems. Currently, the imaging models of most XLCT systems assume that nanophosphors emit light based on the intensity distribution of x-ray within the object, not completely reflecting the nature of the x-ray excitation process. To improve the imaging quality of CB-XLCT, an imaging model that adopts an excitation model of nanophosphors based on x-ray absorption dosage is proposed in this study. To solve the ill-posed inverse problem, a reconstruction algorithm that combines the adaptive Tikhonov regularization method with the imaging model is implemented for CB-XLCT reconstruction. Numerical simulations and phantom experiments indicate that compared with the traditional forward model based on x-ray intensity, the proposed dose-based model could improve the image quality of CB-XLCT significantly in terms of target shape, localization accuracy, and image contrast. In addition, the proposed model behaves better in distinguishing closer targets, demonstrating its advantage in improving spatial resolution.

Multi-spectral endogenous fluorescence imaging for bacterial differentiation

NASA Astrophysics Data System (ADS)

Chernomyrdin, Nikita V.; Babayants, Margarita V.; Korotkov, Oleg V.; Kudrin, Konstantin G.; Rimskaya, Elena N.; Shikunova, Irina A.; Kurlov, Vladimir N.; Cherkasova, Olga P.; Komandin, Gennady A.; Reshetov, Igor V.; Zaytsev, Kirill I.

2017-07-01

In this paper, the multi-spectral endogenous fluorescence imaging was implemented for bacterial differentiation. The fluorescence imaging was performed using a digital camera equipped with a set of visual bandpass filters. Narrowband 365 nm ultraviolet radiation passed through a beam homogenizer was used to excite the sample fluorescence. In order to increase a signal-to-noise ratio and suppress a non-fluorescence background in images, the intensity of the UV excitation was modulated using a mechanical chopper. The principal components were introduced for differentiating the samples of bacteria based on the multi-spectral endogenous fluorescence images.

An edge preserving differential image coding scheme

NASA Technical Reports Server (NTRS)

Rost, Martin C.; Sayood, Khalid

1992-01-01

Differential encoding techniques are fast and easy to implement. However, a major problem with the use of differential encoding for images is the rapid edge degradation encountered when using such systems. This makes differential encoding techniques of limited utility, especially when coding medical or scientific images, where edge preservation is of utmost importance. A simple, easy to implement differential image coding system with excellent edge preservation properties is presented. The coding system can be used over variable rate channels, which makes it especially attractive for use in the packet network environment.

Eliminating Unwanted Far-Field Excitation in Objective-Type TIRF. Part II. Combined Evanescent-Wave Excitation and Supercritical-Angle Fluorescence Detection Improves Optical Sectioning

PubMed Central

Brunstein, Maia; Hérault, Karine; Oheim, Martin

2014-01-01

Azimuthal beam scanning makes evanescent-wave (EW) excitation isotropic, thereby producing total internal reflection fluorescence (TIRF) images that are evenly lit. However, beam spinning does not fundamentally address the problem of propagating excitation light that is contaminating objective-type TIRF. Far-field excitation depends more on the specific objective than on cell scattering. As a consequence, the excitation impurities in objective-type TIRF are only weakly affected by changes of azimuthal or polar beam angle. These are the main results of the first part of this study (Eliminating unwanted far-field excitation in objective-type TIRF. Pt.1. Identifying sources of nonevanescent excitation light). This second part focuses on exactly where up beam in the illumination system stray light is generated that gives rise to nonevanescent components in TIRF. Using dark-field imaging of scattered excitation light we pinpoint the objective, intermediate lenses and, particularly, the beam scanner as the major sources of stray excitation. We study how adhesion-molecule coating and astrocytes or BON cells grown on the coverslip surface modify the dark-field signal. On flat and weakly scattering cells, most background comes from stray reflections produced far from the sample plane, in the beam scanner and the objective lens. On thick, optically dense cells roughly half of the scatter is generated by the sample itself. We finally show that combining objective-type EW excitation with supercritical-angle fluorescence (SAF) detection efficiently rejects the fluorescence originating from deeper sample regions. We demonstrate that SAF improves the surface selectivity of TIRF, even at shallow penetration depths. The coplanar microscopy scheme presented here merges the benefits of beam spinning EW excitation and SAF detection and provides the conditions for quantitative wide-field imaging of fluorophore dynamics at or near the plasma membrane. PMID:24606929

Subband coding for image data archiving

NASA Technical Reports Server (NTRS)

Glover, Daniel; Kwatra, S. C.

1993-01-01

The use of subband coding on image data is discussed. An overview of subband coding is given. Advantages of subbanding for browsing and progressive resolution are presented. Implementations for lossless and lossy coding are discussed. Algorithm considerations and simple implementations of subband systems are given.

Subband coding for image data archiving

NASA Technical Reports Server (NTRS)

Glover, D.; Kwatra, S. C.

1992-01-01

The use of subband coding on image data is discussed. An overview of subband coding is given. Advantages of subbanding for browsing and progressive resolution are presented. Implementations for lossless and lossy coding are discussed. Algorithm considerations and simple implementations of subband are given.

Advanced Imaging Optics Utilizing Wavefront Coding.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scrymgeour, David; Boye, Robert; Adelsberger, Kathleen

2015-06-01

Image processing offers a potential to simplify an optical system by shifting some of the imaging burden from lenses to the more cost effective electronics. Wavefront coding using a cubic phase plate combined with image processing can extend the system's depth of focus, reducing many of the focus-related aberrations as well as material related chromatic aberrations. However, the optimal design process and physical limitations of wavefront coding systems with respect to first-order optical parameters and noise are not well documented. We examined image quality of simulated and experimental wavefront coded images before and after reconstruction in the presence of noise.more » Challenges in the implementation of cubic phase in an optical system are discussed. In particular, we found that limitations must be placed on system noise, aperture, field of view and bandwidth to develop a robust wavefront coded system.« less

Multimodal wide-field two-photon excitation imaging: characterization of the technique for in vivo applications

PubMed Central

Hwang, Jae Youn; Wachsmann-Hogiu, Sebastian; Ramanujan, V Krishnan; Nowatzyk, Andreas G.; Koronyo, Yosef; Medina-Kauwe, Lali K.; Gross, Zeev; Gray, Harry B.; Farkas, Daniel L.

2011-01-01

We report fast, non-scanning, wide-field two-photon fluorescence excitation with spectral and lifetime detection for in vivo biomedical applications. We determined the optical characteristics of the technique, developed a Gaussian flat-field correction method to reduce artifacts resulting from non-uniform excitation such that contrast is enhanced, and showed that it can be used for ex vivo and in vivo cellular-level imaging. Two applications were demonstrated: (i) ex vivo measurements of beta-amyloid plaques in retinas of transgenic mice, and (ii) in vivo imaging of sulfonated gallium(III) corroles injected into tumors. We demonstrate that wide-field two photon fluorescence excitation with flat-field correction provides more penetration depth as well as better contrast and axial resolution than the corresponding one-photon wide field excitation for the same dye. Importantly, when this technique is used together with spectral and fluorescence lifetime detection modules, it offers improved discrimination between fluorescence from molecules of interest and autofluorescence, with higher sensitivity and specificity for in vivo applications. PMID:21339880

A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo

PubMed Central

Chu, Jun; Oh, Young-Hee; Sens, Alex; Ataie, Niloufar; Dana, Hod; Macklin, John J.; Laviv, Tal; Welf, Erik S.; Dean, Kevin M.; Zhang, Feijie; Kim, Benjamin B.; Tang, Clement Tran; Hu, Michelle; Baird, Michelle A.; Davidson, Michael W.; Kay, Mark A.; Fiolka, Reto; Yasuda, Ryohei; Kim, Douglas S.; Ng, Ho-Leung; Lin, Michael Z.

2016-01-01

Orange-red fluorescent proteins (FPs) are widely used in biomedical research for multiplexed epifluorescence microscopy with GFP-based probes, but their different excitation requirements make multiplexing with new advanced microscopy methods difficult. Separately, orange-red FPs are useful for deep-tissue imaging in mammals due to the relative tissue transmissibility of orange-red light, but their dependence on illumination limits their sensitivity as reporters in deep tissues. Here we describe CyOFP1, a bright engineered orange-red FP that is excitable by cyan light. We show that CyOFP1 enables single-excitation multiplexed imaging with GFP-based probes in single-photon and two-photon microscopy, including time-lapse imaging in light-sheet systems. CyOFP1 also serves as an efficient acceptor for resonance energy transfer from the highly catalytic blue-emitting luciferase NanoLuc. An optimized fusion of CyOFP1 and NanoLuc, called Antares, functions as a highly sensitive bioluminescent reporter in vivo, producing substantially brighter signals from deep tissues than firefly luciferase and other bioluminescent proteins. PMID:27240196

Excitation-resolved multispectral method for imaging pharmacokinetic parameters in dynamic fluorescent molecular tomography

NASA Astrophysics Data System (ADS)

Chen, Maomao; Zhou, Yuan; Su, Han; Zhang, Dong; Luo, Jianwen

2017-04-01

Imaging of the pharmacokinetic parameters in dynamic fluorescence molecular tomography (DFMT) can provide three-dimensional metabolic information for biological studies and drug development. However, owing to the ill-posed nature of the FMT inverse problem, the relatively low quality of the parametric images makes it difficult to investigate the different metabolic processes of the fluorescent targets with small distances. An excitation-resolved multispectral DFMT method is proposed; it is based on the fact that the fluorescent targets with different concentrations show different variations in the excitation spectral domain and can be considered independent signal sources. With an independent component analysis method, the spatial locations of different fluorescent targets can be decomposed, and the fluorescent yields of the targets at different time points can be recovered. Therefore, the metabolic process of each component can be independently investigated. Simulations and phantom experiments are carried out to evaluate the performance of the proposed method. The results demonstrated that the proposed excitation-resolved multispectral method can effectively improve the reconstruction accuracy of the parametric images in DFMT.

Long-depth imaging of specific gene expressions in whole-mount mouse embryos with single-photon excitation confocal fluorescence microscopy and FISH.

PubMed

Palmes-Saloma, C; Saloma, C

2000-07-01

Long-depth imaging of specific gene expression in the midgestation whole-mount mouse embryo (WME) is demonstrated with single-photon excitation (1PE) confocal fluorescence microscopy and fluorescence in situ hybridization. Expression domains of Pax-6 mRNA transcripts were labeled with an in situ hybridization probe that is a RNA sequence complementary to the cloned gene fragment and were rendered visible using two fluorochrome-conjugated antibodies that fluoresce at peak wavelengths of lambda(F) = 0.525 microm and lambda(F) = 0. 580 microm, respectively. Distributions of Pax-6 mRNA domains as deep as 1000 microm in the day 9.5 WME were imaged with a long-working-distance (13.6 mm) objective lens (magnification 5x). The scattering problem posed by the optically thick WME sample is alleviated by careful control of the detector pinhole size and the application of simple but fast postdetection image enhancement techniques, such as space and wavelength averaging to produce high-quality fluorescence images. A three-dimensional reconstruction that clearly shows the Pax-6 mRNA expression domains in the forebrain, diencephalon, optic cup, and spinal cord of the day 9.5 WME is obtained. The advantages of 1PE confocal fluorescence imaging over two-photon excitation fluorescence imaging are discussed for the case of long-depth imaging in highly scattering media. Imaging in midgestation WMEs at optical depths of more than 350 microm has not yet been realized with two-photon fluorescence excitation. Copyright 2000 Academic Press.

Polymer dots enable deep in vivo multiphoton fluorescence imaging of cerebrovascular architecture

NASA Astrophysics Data System (ADS)

Hassan, Ahmed M.; Wu, Xu; Jarrett, Jeremy W.; Xu, Shihan; Miller, David R.; Yu, Jiangbo; Perillo, Evan P.; Liu, Yen-Liang; Chiu, Daniel T.; Yeh, Hsin-Chih; Dunn, Andrew K.

2018-02-01

Deep in vivo imaging of vasculature requires small, bright, and photostable fluorophores suitable for multiphoton microscopy (MPM). Although semiconducting polymer dots (pdots) are an emerging class of highly fluorescent contrast agents with favorable advantages for the next generation of in vivo imaging, their use for deep multiphoton imaging has never before been demonstrated. Here we characterize the multiphoton properties of three pdot variants (CNPPV, PFBT, and PFPV) and demonstrate deep imaging of cortical microvasculature in C57 mice. Specifically, we measure the two- versus three-photon power dependence of these pdots and observe a clear three-photon excitation signature at wavelengths longer than 1300 nm, and a transition from two-photon to three-photon excitation within a 1060 - 1300 nm excitation range. Furthermore, we show that pdots enable in vivo two-photon imaging of cerebrovascular architecture in mice up to 850 μm beneath the pial surface using 800 nm excitation. In contrast with traditional multiphoton probes, we also demonstrate that the broad multiphoton absorption spectrum of pdots permits imaging at longer wavelengths (λex = 1,060 and 1225 nm). These wavelengths approach an ideal biological imaging wavelength near 1,300 nm and confer compatibility with a high-power ytterbium-fiber laser and a high pulse energy optical parametric amplifier, resulting in substantial improvements in signal-to-background ratio (>3.5-fold) and greater cortical imaging depths of 900 μm and 1300 μm. Ultimately, pdots are a versatile tool for MPM due to their extraordinary brightness and broad absorption, which will undoubtedly unlock the ability to interrogate deep structures in vivo.

A small animal time-resolved optical tomography platform using wide-field excitation

NASA Astrophysics Data System (ADS)

Venugopal, Vivek

Small animal imaging plays a critical role in present day biomedical research by filling an important gap in the translation of research from the bench to the bedside. Optical techniques constitute an emerging imaging modality which have tremendous potential in preclinical applications. Optical imaging methods are capable of non-invasive assessment of the functional and molecular characteristics of biological tissue. The three-dimensional optical imaging technique, referred to as diffuse optical tomography, provides an approach for the whole-body imaging of small animal models and can provide volumetric maps of tissue functional parameters (e.g. blood volume, oxygen saturation etc.) and/or provide 3D localization and quantification of fluorescence-based molecular markers in vivo. However, the complex mathematical reconstruction problem associated with optical tomography and the cumbersome instrumental designs limits its adoption as a high-throughput quantitative whole-body imaging modality in current biomedical research. The development of new optical imaging paradigms is thus necessary for a wide-acceptance of this new technology. In this thesis, the design, development, characterization and optimization of a small animal optical tomography system is discussed. Specifically, the platform combines a highly sensitive time-resolved imaging paradigm with multi-spectral excitation capability and CCD-based detection to provide a system capable of generating spatially, spectrally and temporally dense measurement datasets. The acquisition of such data sets however can take long and translate to often unrealistic acquisition times when using the classical point source based excitation scheme. The novel approach in the design of this platform is the adoption of a wide-field excitation scheme which employs extended excitation sources and in the process allows an estimated ten-fold reduction in the acquisition time. The work described herein details the design of the imaging platform employing DLP-based excitation and time-gated intensified CCD detection and the optimal system operation parameters are determined. The feasibility this imaging approach and accuracy of the system in reconstructing functional parameters and fluorescence markers based on lifetime contrast is established through phantom studies. As a part of the system characterization, the effect of noise in time-resolved optical tomography is investigated and propagation of system noise in optical reconstructions is established. Furthermore, data processing and measurement calibration techniques aimed at reducing the effect of noise in reconstructions are defined. The optimization of excitation pattern selection is established through a novel measurement-guided iterative pattern correction scheme. This technique referred to as Adaptive Full-Field Optical Tomography was shown to improve reconstruction performances in murine models by reducing the dynamic range in photon flux measurements on the surface. Lastly, the application of the unique attributes of this platform to a biologically relevant imaging application, referred to as Forster Resonance Energy Transfer is described. The tomographic imaging of FRET interaction in vivo on a whole-body scale is achieved using the wide-field imaging approach based on lifetime contrast. This technique represents the first demonstration of tomographic FRET imaging in small animals and has significant potential in the development of optical imaging techniques in varied applications ranging from drug discovery to in vivo study of protein-protein interaction.

Coding and transmission of subband coded images on the Internet

NASA Astrophysics Data System (ADS)

Wah, Benjamin W.; Su, Xiao

2001-09-01

Subband-coded images can be transmitted in the Internet using either the TCP or the UDP protocol. Delivery by TCP gives superior decoding quality but with very long delays when the network is unreliable, whereas delivery by UDP has negligible delays but with degraded quality when packets are lost. Although images are delivered currently over the Internet by TCP, we study in this paper the use of UDP to deliver multi-description reconstruction-based subband-coded images. First, in order to facilitate recovery from UDP packet losses, we propose a joint sender-receiver approach for designing optimized reconstruction-based subband transform (ORB-ST) in multi-description coding (MDC). Second, we carefully evaluate the delay-quality trade-offs between the TCP delivery of SDC images and the UDP and combined TCP/UDP delivery of MDC images. Experimental results show that our proposed ORB-ST performs well in real Internet tests, and UDP and combined TCP/UDP delivery of MDC images provide a range of attractive alternatives to TCP delivery.

Three-dimensional holoscopic image coding scheme using high-efficiency video coding with kernel-based minimum mean-square-error estimation

NASA Astrophysics Data System (ADS)

Liu, Deyang; An, Ping; Ma, Ran; Yang, Chao; Shen, Liquan; Li, Kai

2016-07-01

Three-dimensional (3-D) holoscopic imaging, also known as integral imaging, light field imaging, or plenoptic imaging, can provide natural and fatigue-free 3-D visualization. However, a large amount of data is required to represent the 3-D holoscopic content. Therefore, efficient coding schemes for this particular type of image are needed. A 3-D holoscopic image coding scheme with kernel-based minimum mean square error (MMSE) estimation is proposed. In the proposed scheme, the coding block is predicted by an MMSE estimator under statistical modeling. In order to obtain the signal statistical behavior, kernel density estimation (KDE) is utilized to estimate the probability density function of the statistical modeling. As bandwidth estimation (BE) is a key issue in the KDE problem, we also propose a BE method based on kernel trick. The experimental results demonstrate that the proposed scheme can achieve a better rate-distortion performance and a better visual rendering quality.

A Model for Atomic and Molecular Interstellar Gas: The Meudon PDR Code

NASA Astrophysics Data System (ADS)

Le Petit, Franck; Nehmé, Cyrine; Le Bourlot, Jacques; Roueff, Evelyne

2006-06-01

We present the revised ``Meudon'' model of photon-dominated region (PDR) code, available on the Web under the GNU Public License. General organization of the code is described down to a level that should allow most observers to use it as an interpretation tool with minimal help from our part. Two grids of models, one for low-excitation diffuse clouds and one for dense highly illuminated clouds, are discussed, and some new results on PDR modelization highlighted.

Motion corrected photoacoustic difference imaging of fluorescent contrast agents

NASA Astrophysics Data System (ADS)

Märk, Julia; Wagener, Asja; Pönick, Sarah; Grötzinger, Carsten; Zhang, Edward; Laufer, Jan

2016-03-01

In fluorophores, such as exogenous dyes and genetically expressed proteins, the excited state lifetime can be modulated using pump-probe excitation at wavelengths corresponding to the absorption and fluorescence spectra. Simultaneous pump-probe pulses induce stimulated emission (SE) which, in turn, modulates the thermalized energy, and hence the photoacoustic (PA) signal amplitude. For time-delayed pulses, by contrast, SE is suppressed. Since this is not observed in endogenous chromophores, the location of the fluorophore can be determined by subtracting images acquired using simultaneous and time-delayed pump-probe excitation. This simple experimental approach exploits a fluorophorespecific contrast mechanism, and has the potential to enable deep-tissue molecular imaging at fluences below the MPE. In this study, some of the challenges to its in vivo implementation are addressed. First, the PA signal amplitude generated in fluorophores in vivo is often much smaller than that in blood. Second, tissue motion can give rise to artifacts that correspond to endogenous chromophores in the difference image. This would not allow the unambiguous detection of fluorophores. A method to suppress motion artifacts based on fast switching between simultaneous and time-delayed pump-probe excitation was developed. This enables the acquisition of PA signals using the two excitation modes with minimal time delay (20 ms), thus minimizing the effects of tissue motion. The feasibility of this method is demonstrated by visualizing a fluorophore (Atto680) in tissue phantoms, which were moved during the image acquisition to mimic tissue motion.

Inducible Knockout of the Cyclin-Dependent Kinase 5 Activator p35 Alters Hippocampal Spatial Coding and Neuronal Excitability

PubMed Central

Kamiki, Eriko; Boehringer, Roman; Polygalov, Denis; Ohshima, Toshio; McHugh, Thomas J.

2018-01-01

p35 is an activating co-factor of Cyclin-dependent kinase 5 (Cdk5), a protein whose dysfunction has been implicated in a wide-range of neurological disorders including cognitive impairment and disease. Inducible deletion of the p35 gene in adult mice results in profound deficits in hippocampal-dependent spatial learning and synaptic physiology, however the impact of the loss of p35 function on hippocampal in vivo physiology and spatial coding remains unknown. Here, we recorded CA1 pyramidal cell activity in freely behaving p35 cKO and control mice and found that place cells in the mutant mice have elevated firing rates and impaired spatial coding, accompanied by changes in the temporal organization of spiking both during exploration and rest. These data shed light on the role of p35 in maintaining cellular and network excitability and provide a physiological correlate of the spatial learning deficits in these mice. PMID:29867369

Plant Cell Imaging Based on Nanodiamonds with Excitation-Dependent Fluorescence

NASA Astrophysics Data System (ADS)

Su, Li-Xia; Lou, Qing; Jiao, Zhen; Shan, Chong-Xin

2016-09-01

Despite extensive work on fluorescence behavior stemming from color centers of diamond, reports on the excitation-dependent fluorescence of nanodiamonds (NDs) with a large-scale redshift from 400 to 620 nm under different excitation wavelengths are so far much fewer, especially in biological applications. The fluorescence can be attributed to the combined effects of the fraction of sp2-hybridized carbon atoms among the surface of the fine diamond nanoparticles and the defect energy trapping states on the surface of the diamond. The excitation-dependent fluorescent NDs have been applied in plant cell imaging for the first time. The results reported in this paper may provide a promising route to multiple-color bioimaging using NDs.

Plant Cell Imaging Based on Nanodiamonds with Excitation-Dependent Fluorescence.

PubMed

Su, Li-Xia; Lou, Qing; Jiao, Zhen; Shan, Chong-Xin

2016-12-01

Despite extensive work on fluorescence behavior stemming from color centers of diamond, reports on the excitation-dependent fluorescence of nanodiamonds (NDs) with a large-scale redshift from 400 to 620 nm under different excitation wavelengths are so far much fewer, especially in biological applications. The fluorescence can be attributed to the combined effects of the fraction of sp(2)-hybridized carbon atoms among the surface of the fine diamond nanoparticles and the defect energy trapping states on the surface of the diamond. The excitation-dependent fluorescent NDs have been applied in plant cell imaging for the first time. The results reported in this paper may provide a promising route to multiple-color bioimaging using NDs.

2-Step scalar deadzone quantization for bitplane image coding.

PubMed

Auli-Llinas, Francesc

2013-12-01

Modern lossy image coding systems generate a quality progressive codestream that, truncated at increasing rates, produces an image with decreasing distortion. Quality progressivity is commonly provided by an embedded quantizer that employs uniform scalar deadzone quantization (USDQ) together with a bitplane coding strategy. This paper introduces a 2-step scalar deadzone quantization (2SDQ) scheme that achieves same coding performance as that of USDQ while reducing the coding passes and the emitted symbols of the bitplane coding engine. This serves to reduce the computational costs of the codec and/or to code high dynamic range images. The main insights behind 2SDQ are the use of two quantization step sizes that approximate wavelet coefficients with more or less precision depending on their density, and a rate-distortion optimization technique that adjusts the distortion decreases produced when coding 2SDQ indexes. The integration of 2SDQ in current codecs is straightforward. The applicability and efficiency of 2SDQ are demonstrated within the framework of JPEG2000.

Ink-constrained halftoning with application to QR codes

NASA Astrophysics Data System (ADS)

Bayeh, Marzieh; Compaan, Erin; Lindsey, Theodore; Orlow, Nathan; Melczer, Stephen; Voller, Zachary

2014-01-01

This paper examines adding visually significant, human recognizable data into QR codes without affecting their machine readability by utilizing known methods in image processing. Each module of a given QR code is broken down into pixels, which are halftoned in such a way as to keep the QR code structure while revealing aspects of the secondary image to the human eye. The loss of information associated to this procedure is discussed, and entropy values are calculated for examples given in the paper. Numerous examples of QR codes with embedded images are included.

Product code optimization for determinate state LDPC decoding in robust image transmission.

PubMed

Thomos, Nikolaos; Boulgouris, Nikolaos V; Strintzis, Michael G

2006-08-01

We propose a novel scheme for error-resilient image transmission. The proposed scheme employs a product coder consisting of low-density parity check (LDPC) codes and Reed-Solomon codes in order to deal effectively with bit errors. The efficiency of the proposed scheme is based on the exploitation of determinate symbols in Tanner graph decoding of LDPC codes and a novel product code optimization technique based on error estimation. Experimental evaluation demonstrates the superiority of the proposed system in comparison to recent state-of-the-art techniques for image transmission.

Planar imaging of OH density distributions in a supersonic combustion tunnel

NASA Technical Reports Server (NTRS)

Quagliaroli, T. M.; Laufer, G.; Krauss, R. H.; Mcdaniel, J. C., Jr.

1993-01-01

Images of absolute OH number density were obtained using planar laser-induced fluorescence (PLIF) in a supersonic H2-air combustion tunnel. A tunable KrF excimer laser was used to excite the Q2(11) ro-vibronic line. Calibration of the PLIF images was obtained by referencing the signal measured in the flame to that obtained by the excitation of OH produced by thermal dissociation of H2O in an atmospheric furnace. Measurement errors due to uncertainty in internal furnace atmospheric conditions and image temperature correction are estimated.

Automatic removal of cosmic ray signatures in Deep Impact images

NASA Astrophysics Data System (ADS)

Ipatov, S. I.; A'Hearn, M. F.; Klaasen, K. P.

The results of recognition of cosmic ray (CR) signatures on single images made during the Deep Impact mission were analyzed for several codes written by several authors. For automatic removal of CR signatures on many images, we suggest using the code imgclean ( http://pdssbn.astro.umd.edu/volume/didoc_0001/document/calibration_software/dical_v5/) written by E. Deutsch as other codes considered do not work properly automatically with a large number of images and do not run to completion for some images; however, other codes can be better for analysis of certain specific images. Sometimes imgclean detects false CR signatures near the edge of a comet nucleus, and it often does not recognize all pixels of long CR signatures. Our code rmcr is the only code among those considered that allows one to work with raw images. For most visual images made during low solar activity at exposure time t > 4 s, the number of clusters of bright pixels on an image per second per sq. cm of CCD was about 2-4, both for dark and normal sky images. At high solar activity, it sometimes exceeded 10. The ratio of the number of CR signatures consisting of n pixels obtained at high solar activity to that at low solar activity was greater for greater n. The number of clusters detected as CR signatures on a single infrared image is by at least a factor of several greater than the actual number of CR signatures; the number of clusters based on analysis of two successive dark infrared frames is in agreement with an expected number of CR signatures. Some glitches of false CR signatures include bright pixels repeatedly present on different infrared images. Our interactive code imr allows a user to choose the regions on a considered image where glitches detected by imgclean as CR signatures are ignored. In other regions chosen by the user, the brightness of some pixels is replaced by the local median brightness if the brightness of these pixels is greater by some factor than the median brightness. The interactive code allows one to delete long CR signatures and prevents removal of false CR signatures near the edge of the nucleus of the comet. The interactive code can be applied to editing any digital images. Results obtained can be used for other missions to comets.

Control of excitation in the fluorescence microscope.

PubMed

Lea, D J; Ward, D J

1979-01-01

In fluorescence microscopy image brightness and contrast and the rate of fading depend upon the intensity of illumination of the specimen. An iris diaphragm or neutral density filters may be used to reduce fluorescence excitation. Also the excitation bandwidth may be varied by using a broad band exciter filter with a set of interchangeable yellow glass filters at the lamphouse.

SiNC: Saliency-injected neural codes for representation and efficient retrieval of medical radiographs

PubMed Central

Sajjad, Muhammad; Mehmood, Irfan; Baik, Sung Wook

2017-01-01

Medical image collections contain a wealth of information which can assist radiologists and medical experts in diagnosis and disease detection for making well-informed decisions. However, this objective can only be realized if efficient access is provided to semantically relevant cases from the ever-growing medical image repositories. In this paper, we present an efficient method for representing medical images by incorporating visual saliency and deep features obtained from a fine-tuned convolutional neural network (CNN) pre-trained on natural images. Saliency detector is employed to automatically identify regions of interest like tumors, fractures, and calcified spots in images prior to feature extraction. Neuronal activation features termed as neural codes from different CNN layers are comprehensively studied to identify most appropriate features for representing radiographs. This study revealed that neural codes from the last fully connected layer of the fine-tuned CNN are found to be the most suitable for representing medical images. The neural codes extracted from the entire image and salient part of the image are fused to obtain the saliency-injected neural codes (SiNC) descriptor which is used for indexing and retrieval. Finally, locality sensitive hashing techniques are applied on the SiNC descriptor to acquire short binary codes for allowing efficient retrieval in large scale image collections. Comprehensive experimental evaluations on the radiology images dataset reveal that the proposed framework achieves high retrieval accuracy and efficiency for scalable image retrieval applications and compares favorably with existing approaches. PMID:28771497

The optimal code searching method with an improved criterion of coded exposure for remote sensing image restoration

NASA Astrophysics Data System (ADS)

He, Lirong; Cui, Guangmang; Feng, Huajun; Xu, Zhihai; Li, Qi; Chen, Yueting

2015-03-01

Coded exposure photography makes the motion de-blurring a well-posed problem. The integration pattern of light is modulated using the method of coded exposure by opening and closing the shutter within the exposure time, changing the traditional shutter frequency spectrum into a wider frequency band in order to preserve more image information in frequency domain. The searching method of optimal code is significant for coded exposure. In this paper, an improved criterion of the optimal code searching is proposed by analyzing relationship between code length and the number of ones in the code, considering the noise effect on code selection with the affine noise model. Then the optimal code is obtained utilizing the method of genetic searching algorithm based on the proposed selection criterion. Experimental results show that the time consuming of searching optimal code decreases with the presented method. The restoration image is obtained with better subjective experience and superior objective evaluation values.

LabVIEW control software for scanning micro-beam X-ray fluorescence spectrometer.

PubMed

Wrobel, Pawel; Czyzycki, Mateusz; Furman, Leszek; Kolasinski, Krzysztof; Lankosz, Marek; Mrenca, Alina; Samek, Lucyna; Wegrzynek, Dariusz

2012-05-15

Confocal micro-beam X-ray fluorescence microscope was constructed. The system was assembled from commercially available components - a low power X-ray tube source, polycapillary X-ray optics and silicon drift detector - controlled by an in-house developed LabVIEW software. A video camera coupled to optical microscope was utilized to display the area excited by X-ray beam. The camera image calibration and scan area definition software were also based entirely on LabVIEW code. Presently, the main area of application of the newly constructed spectrometer is 2-dimensional mapping of element distribution in environmental, biological and geological samples with micrometer spatial resolution. The hardware and the developed software can already handle volumetric 3-D confocal scans. In this work, a front panel graphical user interface as well as communication protocols between hardware components were described. Two applications of the spectrometer, to homogeneity testing of titanium layers and to imaging of various types of grains in air particulate matter collected on membrane filters, were presented. Copyright © 2012 Elsevier B.V. All rights reserved.

Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields

PubMed Central

Rickgauer, John Peter; Deisseroth, Karl; Tank, David W.

2015-01-01

Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron’s coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, λ = 920 ± 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, λ = 1,064 ± 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or ‘biasing’, to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields. PMID:25402854

A Degree Distribution Optimization Algorithm for Image Transmission

NASA Astrophysics Data System (ADS)

Jiang, Wei; Yang, Junjie

2016-09-01

Luby Transform (LT) code is the first practical implementation of digital fountain code. The coding behavior of LT code is mainly decided by the degree distribution which determines the relationship between source data and codewords. Two degree distributions are suggested by Luby. They work well in typical situations but not optimally in case of finite encoding symbols. In this work, the degree distribution optimization algorithm is proposed to explore the potential of LT code. Firstly selection scheme of sparse degrees for LT codes is introduced. Then probability distribution is optimized according to the selected degrees. In image transmission, bit stream is sensitive to the channel noise and even a single bit error may cause the loss of synchronization between the encoder and the decoder. Therefore the proposed algorithm is designed for image transmission situation. Moreover, optimal class partition is studied for image transmission with unequal error protection. The experimental results are quite promising. Compared with LT code with robust soliton distribution, the proposed algorithm improves the final quality of recovered images obviously with the same overhead.

Illusory Motion Reproduced by Deep Neural Networks Trained for Prediction

PubMed Central

Watanabe, Eiji; Kitaoka, Akiyoshi; Sakamoto, Kiwako; Yasugi, Masaki; Tanaka, Kenta

2018-01-01

The cerebral cortex predicts visual motion to adapt human behavior to surrounding objects moving in real time. Although the underlying mechanisms are still unknown, predictive coding is one of the leading theories. Predictive coding assumes that the brain's internal models (which are acquired through learning) predict the visual world at all times and that errors between the prediction and the actual sensory input further refine the internal models. In the past year, deep neural networks based on predictive coding were reported for a video prediction machine called PredNet. If the theory substantially reproduces the visual information processing of the cerebral cortex, then PredNet can be expected to represent the human visual perception of motion. In this study, PredNet was trained with natural scene videos of the self-motion of the viewer, and the motion prediction ability of the obtained computer model was verified using unlearned videos. We found that the computer model accurately predicted the magnitude and direction of motion of a rotating propeller in unlearned videos. Surprisingly, it also represented the rotational motion for illusion images that were not moving physically, much like human visual perception. While the trained network accurately reproduced the direction of illusory rotation, it did not detect motion components in negative control pictures wherein people do not perceive illusory motion. This research supports the exciting idea that the mechanism assumed by the predictive coding theory is one of basis of motion illusion generation. Using sensory illusions as indicators of human perception, deep neural networks are expected to contribute significantly to the development of brain research. PMID:29599739

Illusory Motion Reproduced by Deep Neural Networks Trained for Prediction.

PubMed

Watanabe, Eiji; Kitaoka, Akiyoshi; Sakamoto, Kiwako; Yasugi, Masaki; Tanaka, Kenta

2018-01-01

The cerebral cortex predicts visual motion to adapt human behavior to surrounding objects moving in real time. Although the underlying mechanisms are still unknown, predictive coding is one of the leading theories. Predictive coding assumes that the brain's internal models (which are acquired through learning) predict the visual world at all times and that errors between the prediction and the actual sensory input further refine the internal models. In the past year, deep neural networks based on predictive coding were reported for a video prediction machine called PredNet. If the theory substantially reproduces the visual information processing of the cerebral cortex, then PredNet can be expected to represent the human visual perception of motion. In this study, PredNet was trained with natural scene videos of the self-motion of the viewer, and the motion prediction ability of the obtained computer model was verified using unlearned videos. We found that the computer model accurately predicted the magnitude and direction of motion of a rotating propeller in unlearned videos. Surprisingly, it also represented the rotational motion for illusion images that were not moving physically, much like human visual perception. While the trained network accurately reproduced the direction of illusory rotation, it did not detect motion components in negative control pictures wherein people do not perceive illusory motion. This research supports the exciting idea that the mechanism assumed by the predictive coding theory is one of basis of motion illusion generation. Using sensory illusions as indicators of human perception, deep neural networks are expected to contribute significantly to the development of brain research.

Ionospheric-thermospheric UV tomography: 3. A multisensor technique for creating full-orbit reconstructions of atmospheric UV emission

NASA Astrophysics Data System (ADS)

Hei, Matthew A.; Budzien, Scott A.; Dymond, Kenneth F.; Nicholas, Andrew C.; Paxton, Larry J.; Schaefer, Robert K.; Groves, Keith M.

2017-07-01

We present the Volume Emission Rate Tomography (VERT) technique for inverting satellite-based, multisensor limb and nadir measurements of atmospheric ultraviolet emission to create whole-orbit reconstructions of atmospheric volume emission rate. The VERT approach is more general than previous ionospheric tomography methods because it can reconstruct the volume emission rate field irrespective of the particular excitation mechanisms (e.g., radiative recombination, photoelectron impact excitation, and energetic particle precipitation in auroras); physical models are then applied to interpret the airglow. The technique was developed and tested using data from the Special Sensor Ultraviolet Limb Imager and Special Sensor Ultraviolet Spectrographic Imager instruments aboard the Defense Meteorological Satellite Program F-18 spacecraft and planned for use with upcoming remote sensing missions. The technique incorporates several features to optimize the tomographic solutions, such as the use of a nonnegative algorithm (Richardson-Lucy, RL) that explicitly accounts for the Poisson statistics inherent in optical measurements, capability to include extinction effects due to resonant scattering and absorption of the photons from the lines of sight, a pseudodiffusion-based regularization scheme implemented between iterations of the RL code to produce smoother solutions, and the capability to estimate error bars on the solutions. Tests using simulated atmospheric emissions verify that the technique performs well in a variety of situations, including daytime, nighttime, and even in the challenging terminator regions. Lastly, we consider ionospheric nightglow and validate reconstructions of the nighttime electron density against Advanced Research Project Agency (ARPA) Long-range Tracking and Identification Radar (ALTAIR) incoherent scatter radar data.

Image authentication using distributed source coding.

PubMed

Lin, Yao-Chung; Varodayan, David; Girod, Bernd

2012-01-01

We present a novel approach using distributed source coding for image authentication. The key idea is to provide a Slepian-Wolf encoded quantized image projection as authentication data. This version can be correctly decoded with the help of an authentic image as side information. Distributed source coding provides the desired robustness against legitimate variations while detecting illegitimate modification. The decoder incorporating expectation maximization algorithms can authenticate images which have undergone contrast, brightness, and affine warping adjustments. Our authentication system also offers tampering localization by using the sum-product algorithm.

High performance optical encryption based on computational ghost imaging with QR code and compressive sensing technique

NASA Astrophysics Data System (ADS)

Zhao, Shengmei; Wang, Le; Liang, Wenqiang; Cheng, Weiwen; Gong, Longyan

2015-10-01

In this paper, we propose a high performance optical encryption (OE) scheme based on computational ghost imaging (GI) with QR code and compressive sensing (CS) technique, named QR-CGI-OE scheme. N random phase screens, generated by Alice, is a secret key and be shared with its authorized user, Bob. The information is first encoded by Alice with QR code, and the QR-coded image is then encrypted with the aid of computational ghost imaging optical system. Here, measurement results from the GI optical system's bucket detector are the encrypted information and be transmitted to Bob. With the key, Bob decrypts the encrypted information to obtain the QR-coded image with GI and CS techniques, and further recovers the information by QR decoding. The experimental and numerical simulated results show that the authorized users can recover completely the original image, whereas the eavesdroppers can not acquire any information about the image even the eavesdropping ratio (ER) is up to 60% at the given measurement times. For the proposed scheme, the number of bits sent from Alice to Bob are reduced considerably and the robustness is enhanced significantly. Meantime, the measurement times in GI system is reduced and the quality of the reconstructed QR-coded image is improved.

Compensation of optical heterogeneity-induced artifacts in fluorescence molecular tomography: theory and in vivo validation

NASA Astrophysics Data System (ADS)

Mohajerani, Pouyan; Adibi, Ali; Kempner, Joshua; Yared, Wael

2009-05-01

We present a method for reduction of image artifacts induced by the optical heterogeneities of tissue in fluorescence molecular tomography (FMT) through identification and compensation of image regions that evidence propagation of emission light through thin or low-absorption tunnels in tissue. The light tunneled as such contributes to the emission image as spurious components that might substantially overwhelm the desirable fluorescence emanating from the targeted lesions. The proposed method makes use of the strong spatial correlation between the emission and excitation images to estimate the tunneled components and yield a residual image that mainly consists of the signal due to the desirable fluorescence. This residual image is further refined using a coincidence mask constructed for each excitation-emission image pair. The coincidence mask is essentially a map of the ``hot spots'' that occur in both excitation and emission images, as such areas are often associated with tunneled emission. In vivo studies are performed on a human colon adenocarcinoma xenograft tumor model with subcutaneous tumors and a murine breast adenocarcinoma model with aggressive tumor cell metastasis and growth in the lungs. Results demonstrate significant improvements in the reconstructions achieved by the proposed method.

Elucidating Neuronal Mechanisms Using Intracellular Recordings during Behavior.

PubMed

Lee, Albert K; Brecht, Michael

2018-06-01

Intracellular recording allows measurement and perturbation of the membrane potential of identified neurons with sub-millisecond and sub-millivolt precision. This gives intracellular recordings a unique capacity to provide rich information about individual cells (e.g., high-resolution characterization of inputs, outputs, excitability, and structure). Hence, such recordings can elucidate the mechanisms that underlie fundamental phenomena, such as brain state, sparse coding, gating, gain modulation, and learning. Technical developments have increased the range of behaviors during which intracellular recording methods can be employed, such as in freely moving animals and head-fixed animals actively performing tasks, including in virtual environments. Such advances, and the combination of intracellular recordings with genetic and imaging techniques, have enabled investigation of the mechanisms that underlie neural computations during natural and trained behaviors. Copyright © 2018 Elsevier Ltd. All rights reserved.

Breakdown of the Wigner-Mattis theorem in semiconductor carbon-nanotube quantum dots

NASA Astrophysics Data System (ADS)

Rontani, Massimo; Secchi, Andrea; Manghi, Franca

2009-03-01

The Wigner-Mattis theorem states the ground state of two bound electrons, in the absence of the magnetic field, is always a spin-singlet. We predict the opposite result --a triplet- for two electrons in a quantum dot defined in a semiconductor carbon nanotube. The claim is supported by extensive many-body calculations based on the accurate configuration interaction code DONRODRIGO (www.s3.infm.t/donrodrigo). The crux of the matter is the peculiar two-valley structure of low-energy states, which encodes a pseudo-spin degree of freedom. The spin polarization of the ground state corresponds to a pseudo-spin singlet, which is selected by the inter-valley short-range Coulomb interaction. Single-electron excitation spectra and STM wave function images may validate this scenario, as shown by our numerical simulations.

Investigating line- versus point-laser excitation for three-dimensional fluorescence imaging and tomography employing a trimodal imaging system

NASA Astrophysics Data System (ADS)

Cao, Liji; Peter, Jörg

2013-06-01

The adoption of axially oriented line illumination patterns for fluorescence excitation in small animals for fluorescence surface imaging (FSI) and fluorescence optical tomography (FOT) is being investigated. A trimodal single-photon-emission-computed-tomography/computed-tomography/optical-tomography (SPECT-CT-OT) small animal imaging system is being modified for employment of point- and line-laser excitation sources. These sources can be arbitrarily positioned around the imaged object. The line source is set to illuminate the object along its entire axial direction. Comparative evaluation of point and line illumination patterns for FSI and FOT is provided involving phantom as well as mouse data. Given the trimodal setup, CT data are used to guide the optical approaches by providing boundary information. Furthermore, FOT results are also being compared to SPECT. Results show that line-laser illumination yields a larger axial field of view (FOV) in FSI mode, hence faster data acquisition, and practically acceptable FOT reconstruction throughout the whole animal. Also, superimposed SPECT and FOT data provide additional information on similarities as well as differences in the distribution and uptake of both probe types. Fused CT data enhance further the anatomical localization of the tracer distribution in vivo. The feasibility of line-laser excitation for three-dimensional fluorescence imaging and tomography is demonstrated for initiating further research, however, not with the intention to replace one by the other.

In vivo three-photon imaging of deep cerebellum

NASA Astrophysics Data System (ADS)

Wang, Mengran; Wang, Tianyu; Wu, Chunyan; Li, Bo; Ouzounov, Dimitre G.; Sinefeld, David; Guru, Akash; Nam, Hyung-Song; Capecchi, Mario R.; Warden, Melissa R.; Xu, Chris

2018-02-01

We demonstrate three-photon microscopy (3PM) of mouse cerebellum at 1 mm depth by imaging both blood vessels and neurons. We compared 3PM and 2PM in the mouse cerebellum for imaging green (using excitation sources at 1300 nm and 920 nm, respectively) and red fluorescence (using excitation sources at 1680 nm and 1064 nm, respectively). 3PM enabled deeper imaging than 2PM because the use of longer excitation wavelength reduces the scattering in biological tissue and the higher order nonlinear excitation provides better 3D localization. To illustrate these two advantages quantitatively, we measured the signal decay as well as the signal-to-background ratio (SBR) as a function of depth. We performed 2-photon imaging from the brain surface all the way down to the area where the SBR reaches 1, while at the same depth, 3PM still has SBR above 30. The segmented decay curve shows that the mouse cerebellum has different effective attenuation lengths at different depths, indicating heterogeneous tissue property for this brain region. We compared the third harmonic generation (THG) signal, which is used to visualize myelinated fibers, with the decay curve. We found that the regions with shorter effective attenuation lengths correspond to the regions with more fibers. Our results indicate that the widespread, non-uniformly distributed myelinated fibers adds heterogeneity to mouse cerebellum, which poses additional challenges in deep imaging of this brain region.

Probing cellular uptake and tracking of differently shaped gelatin-coated gold nanoparticles inside of ovarian cancer cells by two-photon excited photoluminescence analyzed by fluorescence lifetime imaging (FLIM).

PubMed

Suarasan, Sorina; Licarete, Emilia; Astilean, Simion; Craciun, Ana-Maria

2018-06-01

Nowadays, the non-linear optical effect of two-photon excited (TPE) fluorescence has recently grown in interest in recent years over other optical imaging method, due to improved 3D spatial resolution, deep penetrability and less photodamage of living organism owing to the excitation in near-infrared region (NIR). In parallel, gold nanoparticles (AuNPs) have gain considerable attention for NIR TPE bio-imaging applications due to their appealing ability to generate strong intrinsic photoluminescence (PL). Here, we demonstrate the capability of differently shaped gelatin-coated AuNPs to perform as reliable label-free contrast agents for the non-invasive NIR imaging of NIH:OVCAR-3 ovary cancer cells via TPE Fluorescence Lifetime Imaging Microscopy (FLIM). Examination of the spectroscopic profile of the intrinsic signals exhibited by AuNPs inside cells confirm the plasmonic nature of the emitted PL, while the evaluation of time-dependent profile of the TPE PL signal under continuous irradiation indicates the photo-stability of the signal revealing simultaneously a photo-blinking behavior. Finally, we assess the dependence of the TPE PL signal on laser excitation power and wavelength in view of contributing to a better understanding of plasmonic TPE PL in biological media towards the improvement of TPE FLIM imaging applications based on AuNPs. Copyright © 2018 Elsevier B.V. All rights reserved.

Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging

PubMed Central

Avti, Pramod K; Sitharaman, Balaji

2012-01-01

Lanthanoid-based optical probes with excitation wavelengths in the ultra-violet (UV) range (300–325 nm) have been widely developed as imaging probes. Efficient cellular imaging requires that lanthanoid optical probes be excited at visible wavelengths, to avoid UV damage to cells. The efficacy of europium-catalyzed single-walled carbon nanotubes (Eu-SWCNTs), as visible nanoprobes for cellular imaging, is reported in this study. Confocal fluorescence microscopy images of breast cancer cells (SK-BR-3 and MCF-7) and normal cells (NIH 3T3), treated with Eu-SWCNT at 0.2 μg/mL concentration, showed bright red luminescence after excitation at 365 nm and 458 nm wavelengths. Cell viability analysis showed no cytotoxic effects after the incubation of cells with Eu-SWCNTs at this concentration. Eu-SWCNT uptake is via the endocytosis mechanism. Labeling efficiency, defined as the percentage of incubated cells that uptake Eu-SWCNT, was 95%–100% for all cell types. The average cellular uptake concentration was 6.68 ng Eu per cell. Intracellular localization was further corroborated by transmission electron microscopy and Raman microscopy. The results indicate that Eu-SWCNT shows potential as a novel cellular imaging probe, wherein SWCNT sensitizes Eu3+ ions to allow excitation at visible wavelengths, and stable time-resolved red emission. The ability to functionalize biomolecules on the exterior surface of Eu-SWCNT makes it an excellent candidate for targeted cellular imaging. PMID:22619533

Intravital multiphoton fluorescence imaging and optical manipulation of spinal cord in mice, using a compact fiber laser system.

PubMed

Oshima, Yusuke; Horiuch, Hideki; Honkura, Naoki; Hikita, Atsuhiko; Ogata, Tadanori; Miura, Hiromasa; Imamura, Takeshi

2014-09-01

Near-infrared ultrafast lasers are widely used for multiphoton excited fluorescence microscopy in living animals. Ti:Sapphire lasers are typically used for multiphoton excitation, but their emission wavelength is restricted below 1,000 nm. The aim of this study is to evaluate the performance of a compact Ytterbium-(Yb-) fiber laser at 1,045 nm for multiphoton excited fluorescence microscopy in spinal cord injury. In this study, we employed a custom-designed microscopy system with a compact Yb-fiber laser and evaluated the performance of this system in in vivo imaging of brain cortex and spinal cord in YFP-H transgenic mice. For in vivo imaging of brain cortex, sharp images of basal dendrites, and pyramidal cells expressing EYFP were successfully captured using the Yb-fiber laser in our microscopy system. We also performed in vivo imaging of axon fibers of spinal cord in the transgenic mice. The obtained images were almost as sharp as those obtained using a conventional ultrafast laser system. In addition, laser ablation and multi-color imaging could be performed simultaneously using the Yb-fiber laser. The high-peak pulse Yb-fiber laser is potentially useful for multimodal bioimaging methods based on a multiphoton excited fluorescence microscopy system that incorporates laser ablation techniques. Our results suggest that microscopy systems of this type could be utilized in studies of neuroscience and clinical use in diagnostics and therapeutic tool for spinal cord injury in the future. © 2014 Wiley Periodicals, Inc.

Three-Dimensional Terahertz Coded-Aperture Imaging Based on Single Input Multiple Output Technology.

PubMed

Chen, Shuo; Luo, Chenggao; Deng, Bin; Wang, Hongqiang; Cheng, Yongqiang; Zhuang, Zhaowen

2018-01-19

As a promising radar imaging technique, terahertz coded-aperture imaging (TCAI) can achieve high-resolution, forward-looking, and staring imaging by producing spatiotemporal independent signals with coded apertures. In this paper, we propose a three-dimensional (3D) TCAI architecture based on single input multiple output (SIMO) technology, which can reduce the coding and sampling times sharply. The coded aperture applied in the proposed TCAI architecture loads either purposive or random phase modulation factor. In the transmitting process, the purposive phase modulation factor drives the terahertz beam to scan the divided 3D imaging cells. In the receiving process, the random phase modulation factor is adopted to modulate the terahertz wave to be spatiotemporally independent for high resolution. Considering human-scale targets, images of each 3D imaging cell are reconstructed one by one to decompose the global computational complexity, and then are synthesized together to obtain the complete high-resolution image. As for each imaging cell, the multi-resolution imaging method helps to reduce the computational burden on a large-scale reference-signal matrix. The experimental results demonstrate that the proposed architecture can achieve high-resolution imaging with much less time for 3D targets and has great potential in applications such as security screening, nondestructive detection, medical diagnosis, etc.

Encryption of QR code and grayscale image in interference-based scheme with high quality retrieval and silhouette problem removal

NASA Astrophysics Data System (ADS)

Qin, Yi; Wang, Hongjuan; Wang, Zhipeng; Gong, Qiong; Wang, Danchen

2016-09-01

In optical interference-based encryption (IBE) scheme, the currently available methods have to employ the iterative algorithms in order to encrypt two images and retrieve cross-talk free decrypted images. In this paper, we shall show that this goal can be achieved via an analytical process if one of the two images is QR code. For decryption, the QR code is decrypted in the conventional architecture and the decryption has a noisy appearance. Nevertheless, the robustness of QR code against noise enables the accurate acquisition of its content from the noisy retrieval, as a result of which the primary QR code can be exactly regenerated. Thereafter, a novel optical architecture is proposed to recover the grayscale image by aid of the QR code. In addition, the proposal has totally eliminated the silhouette problem existing in the previous IBE schemes, and its effectiveness and feasibility have been demonstrated by numerical simulations.

Diffusion Tractography of the Entire Left Ventricle by Using Free-breathing Accelerated Simultaneous Multisection Imaging

PubMed Central

Reese, Timothy G.; Jackowski, Marcel P.; Cauley, Stephen F.; Setsompop, Kawin; Bhat, Himanshu; Sosnovik, David E.

2017-01-01

Purpose To develop a clinically feasible whole-heart free-breathing diffusion-tensor (DT) magnetic resonance (MR) imaging approach with an imaging time of approximately 15 minutes to enable three-dimensional (3D) tractography. Materials and Methods The study was compliant with HIPAA and the institutional review board and required written consent from the participants. DT imaging was performed in seven healthy volunteers and three patients with pulmonary hypertension by using a stimulated echo sequence. Twelve contiguous short-axis sections and six four-chamber sections that covered the entire left ventricle were acquired by using simultaneous multisection (SMS) excitation with a blipped-controlled aliasing in parallel imaging readout. Rate 2 and rate 3 SMS excitation was defined as two and three times accelerated in the section axis, respectively. Breath-hold and free-breathing images with and without SMS acceleration were acquired. Diffusion-encoding directions were acquired sequentially, spatiotemporally registered, and retrospectively selected by using an entropy-based approach. Myofiber helix angle, mean diffusivity, fractional anisotropy, and 3D tractograms were analyzed by using paired t tests and analysis of variance. Results No significant differences (P > .63) were seen between breath-hold rate 3 SMS and free-breathing rate 2 SMS excitation in transmural myofiber helix angle, mean diffusivity (mean ± standard deviation, [0.89 ± 0.09] × 10−3 mm2/sec vs [0.9 ± 0.09] × 10−3 mm2/sec), or fractional anisotropy (0.43 ± 0.05 vs 0.42 ± 0.06). Three-dimensional tractograms of the left ventricle with no SMS and rate 2 and rate 3 SMS excitation were qualitatively similar. Conclusion Free-breathing DT imaging of the entire human heart can be performed in approximately 15 minutes without section gaps by using SMS excitation with a blipped-controlled aliasing in parallel imaging readout, followed by spatiotemporal registration and entropy-based retrospective image selection. This method may lead to clinical translation of whole-heart DT imaging, enabling broad application in patients with cardiac disease. © RSNA, 2016 Online supplemental material is available for this article. PMID:27681278

Diffusion Tractography of the Entire Left Ventricle by Using Free-breathing Accelerated Simultaneous Multisection Imaging.

PubMed

Mekkaoui, Choukri; Reese, Timothy G; Jackowski, Marcel P; Cauley, Stephen F; Setsompop, Kawin; Bhat, Himanshu; Sosnovik, David E

2017-03-01

Purpose To develop a clinically feasible whole-heart free-breathing diffusion-tensor (DT) magnetic resonance (MR) imaging approach with an imaging time of approximately 15 minutes to enable three-dimensional (3D) tractography. Materials and Methods The study was compliant with HIPAA and the institutional review board and required written consent from the participants. DT imaging was performed in seven healthy volunteers and three patients with pulmonary hypertension by using a stimulated echo sequence. Twelve contiguous short-axis sections and six four-chamber sections that covered the entire left ventricle were acquired by using simultaneous multisection (SMS) excitation with a blipped-controlled aliasing in parallel imaging readout. Rate 2 and rate 3 SMS excitation was defined as two and three times accelerated in the section axis, respectively. Breath-hold and free-breathing images with and without SMS acceleration were acquired. Diffusion-encoding directions were acquired sequentially, spatiotemporally registered, and retrospectively selected by using an entropy-based approach. Myofiber helix angle, mean diffusivity, fractional anisotropy, and 3D tractograms were analyzed by using paired t tests and analysis of variance. Results No significant differences (P > .63) were seen between breath-hold rate 3 SMS and free-breathing rate 2 SMS excitation in transmural myofiber helix angle, mean diffusivity (mean ± standard deviation, [0.89 ± 0.09] × 10 -3 mm 2 /sec vs [0.9 ± 0.09] × 10 -3 mm 2 /sec), or fractional anisotropy (0.43 ± 0.05 vs 0.42 ± 0.06). Three-dimensional tractograms of the left ventricle with no SMS and rate 2 and rate 3 SMS excitation were qualitatively similar. Conclusion Free-breathing DT imaging of the entire human heart can be performed in approximately 15 minutes without section gaps by using SMS excitation with a blipped-controlled aliasing in parallel imaging readout, followed by spatiotemporal registration and entropy-based retrospective image selection. This method may lead to clinical translation of whole-heart DT imaging, enabling broad application in patients with cardiac disease. © RSNA, 2016 Online supplemental material is available for this article.

The effect of excitation and preparation pulses on nonslice selective 2D UTE bicomponent analysis of bound and free water in cortical bone at 3T

PubMed Central

Li, Shihong; Chang, Eric Y.; Bae, Won C.; Chung, Christine B.; Hua, Yanqing; Zhou, Yi; Du, Jiang

2014-01-01

Purpose: The purpose of this study was to investigate the effect of excitation, fat saturation, long T2 saturation, and adiabatic inversion pulses on ultrashort echo time (UTE) imaging with bicomponent analysis of bound and free water in cortical bone for potential applications in osteoporosis. Methods: Six bovine cortical bones and six human tibial midshaft samples were harvested for this study. Each bone sample was imaged with eight sequences using 2D UTE imaging at 3T with half and hard excitation pulses, without and with fat saturation, long T2 saturation, and adiabatic inversion recovery (IR) preparation pulses. Single- and bicomponent signal models were utilized to calculate the T2*s and/or relative fractions of short and long T2*s. Results: For all bone samples UTE T2* signal decay showed bicomponent behavior. A higher short T2* fraction was observed on UTE images with hard pulse excitation compared with half pulse excitation (75.6% vs 68.8% in bovine bone, 79.9% vs 73.2% in human bone). Fat saturation pulses slightly reduced the short T2* fraction relative to regular UTE sequences (5.0% and 2.0% reduction, respectively, with half and hard excitation pulses for bovine bone, 6.3% and 8.2% reduction, respectively, with half and hard excitation pulses for human bone). Long T2 saturation pulses significantly reduced the long T2* fraction relative to regular UTE sequence (18.9% and 17.2% reduction, respectively, with half and hard excitation pulses for bovine bone, 26.4% and 27.7% reduction, respectively, with half and hard excitation pulses for human bone). With IR-UTE preparation the long T2* components were significantly reduced relative to regular UTE sequence (75.3% and 66.4% reduction, respectively, with half and hard excitation pulses for bovine bone, 87.7% and 90.3% reduction, respectively, with half and hard excitation pulses for human bone). Conclusions: Bound and free water T2*s and relative fractions can be assessed using UTE bicomponent analysis. Long T2* components are affected more by long T2 saturation and IR pulses, and short T2* components are affected more by fat saturation pulses. PMID:24506644

The effect of excitation and preparation pulses on nonslice selective 2D UTE bicomponent analysis of bound and free water in cortical bone at 3T

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Shihong; Department of Radiology, Hua Dong Hospital, Fudan University, Shanghai 200040; Yancheng Medical College, Jiangsu

Purpose: The purpose of this study was to investigate the effect of excitation, fat saturation, long T2 saturation, and adiabatic inversion pulses on ultrashort echo time (UTE) imaging with bicomponent analysis of bound and free water in cortical bone for potential applications in osteoporosis. Methods: Six bovine cortical bones and six human tibial midshaft samples were harvested for this study. Each bone sample was imaged with eight sequences using 2D UTE imaging at 3T with half and hard excitation pulses, without and with fat saturation, long T2 saturation, and adiabatic inversion recovery (IR) preparation pulses. Single- and bicomponent signal modelsmore » were utilized to calculate the T2{sup *}s and/or relative fractions of short and long T2{sup *}s. Results: For all bone samples UTE T2{sup *} signal decay showed bicomponent behavior. A higher short T2{sup *} fraction was observed on UTE images with hard pulse excitation compared with half pulse excitation (75.6% vs 68.8% in bovine bone, 79.9% vs 73.2% in human bone). Fat saturation pulses slightly reduced the short T2{sup *} fraction relative to regular UTE sequences (5.0% and 2.0% reduction, respectively, with half and hard excitation pulses for bovine bone, 6.3% and 8.2% reduction, respectively, with half and hard excitation pulses for human bone). Long T2 saturation pulses significantly reduced the long T2{sup *} fraction relative to regular UTE sequence (18.9% and 17.2% reduction, respectively, with half and hard excitation pulses for bovine bone, 26.4% and 27.7% reduction, respectively, with half and hard excitation pulses for human bone). With IR-UTE preparation the long T2{sup *} components were significantly reduced relative to regular UTE sequence (75.3% and 66.4% reduction, respectively, with half and hard excitation pulses for bovine bone, 87.7% and 90.3% reduction, respectively, with half and hard excitation pulses for human bone). Conclusions: Bound and free water T2{sup *}s and relative fractions can be assessed using UTE bicomponent analysis. Long T2{sup *} components are affected more by long T2 saturation and IR pulses, and short T2{sup *} components are affected more by fat saturation pulses.« less

In vivo ratiometric Zn2+ imaging in zebrafish larvae using a new visible light excitable fluorescent sensor.

PubMed

Liu, Zhipeng; Zhang, Changli; Chen, Yuncong; Qian, Fang; Bai, Yang; He, Weijiang; Guo, Zijian

2014-02-07

A visible light excitable ratiometric Zn(2+) sensor was developed by integrating a Zn(2+) chelator as the ICT donor of the fluorophore sulfamoylbenzoxadiazole, which displays the Zn(2+)-induced hypsochromic emission shift (40 nm) and favors the in vivo ratiometric Zn(2+) imaging in zebrafish larvae.

Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging

PubMed Central

Zhao, Ming; Li, Yu; Peng, Leilei

2014-01-01

We present a novel excitation-emission multiplexed fluorescence lifetime microscopy (FLIM) method that surpasses current FLIM techniques in multiplexing capability. The method employs Fourier multiplexing to simultaneously acquire confocal fluorescence lifetime images of multiple excitation wavelength and emission color combinations at 44,000 pixels/sec. The system is built with low-cost CW laser sources and standard PMTs with versatile spectral configuration, which can be implemented as an add-on to commercial confocal microscopes. The Fourier lifetime confocal method allows fast multiplexed FLIM imaging, which makes it possible to monitor multiple biological processes in live cells. The low cost and compatibility with commercial systems could also make multiplexed FLIM more accessible to biological research community. PMID:24921725

Rapid 3D bioprinting from medical images: an application to bone scaffolding

NASA Astrophysics Data System (ADS)

Lee, Daniel Z.; Peng, Matthew W.; Shinde, Rohit; Khalid, Arbab; Hong, Abigail; Pennacchi, Sara; Dawit, Abel; Sipzner, Daniel; Udupa, Jayaram K.; Rajapakse, Chamith S.

2018-03-01

Bioprinting of tissue has its applications throughout medicine. Recent advances in medical imaging allows the generation of 3-dimensional models that can then be 3D printed. However, the conventional method of converting medical images to 3D printable G-Code instructions has several limitations, namely significant processing time for large, high resolution images, and the loss of microstructural surface information from surface resolution and subsequent reslicing. We have overcome these issues by creating a JAVA program that skips the intermediate triangularization and reslicing steps and directly converts binary dicom images into G-Code. In this study, we tested the two methods of G-Code generation on the application of synthetic bone graft scaffold generation. We imaged human cadaveric proximal femurs at an isotropic resolution of 0.03mm using a high resolution peripheral quantitative computed tomography (HR-pQCT) scanner. These images, of the Digital Imaging and Communications in Medicine (DICOM) format, were then processed through two methods. In each method, slices and regions of print were selected, filtered to generate a smoothed image, and thresholded. In the conventional method, these processed images are converted to the STereoLithography (STL) format and then resliced to generate G-Code. In the new, direct method, these processed images are run through our JAVA program and directly converted to G-Code. File size, processing time, and print time were measured for each. We found that this new method produced a significant reduction in G-Code file size as well as processing time (92.23% reduction). This allows for more rapid 3D printing from medical images.

Impact of energetic-particle-driven geodesic acoustic modes on turbulence.

PubMed

Zarzoso, D; Sarazin, Y; Garbet, X; Dumont, R; Strugarek, A; Abiteboul, J; Cartier-Michaud, T; Dif-Pradalier, G; Ghendrih, Ph; Grandgirard, V; Latu, G; Passeron, C; Thomine, O

2013-03-22

The impact on turbulent transport of geodesic acoustic modes excited by energetic particles is evidenced for the first time in flux-driven 5D gyrokinetic simulations using the Gysela code. Energetic geodesic acoustic modes (EGAMs) are excited in a regime with a transport barrier in the outer radial region. The interaction between EGAMs and turbulence is such that turbulent transport can be enhanced in the presence of EGAMs, with the subsequent destruction of the transport barrier. This scenario could be particularly critical in those plasmas, such as burning plasmas, exhibiting a rich population of suprathermal particles capable of exciting energetic modes.

Simultaneous Excitation of Multiple-Input Multiple-Output CFD-Based Unsteady Aerodynamic Systems

NASA Technical Reports Server (NTRS)

Silva, Walter A.

2008-01-01

A significant improvement to the development of CFD-based unsteady aerodynamic reduced-order models (ROMs) is presented. This improvement involves the simultaneous excitation of the structural modes of the CFD-based unsteady aerodynamic system that enables the computation of the unsteady aerodynamic state-space model using a single CFD execution, independent of the number of structural modes. Four different types of inputs are presented that can be used for the simultaneous excitation of the structural modes. Results are presented for a flexible, supersonic semi-span configuration using the CFL3Dv6.4 code.

Simultaneous Excitation of Multiple-Input Multiple-Output CFD-Based Unsteady Aerodynamic Systems

NASA Technical Reports Server (NTRS)

Silva, Walter A.

2007-01-01

A significant improvement to the development of CFD-based unsteady aerodynamic reduced-order models (ROMs) is presented. This improvement involves the simultaneous excitation of the structural modes of the CFD-based unsteady aerodynamic system that enables the computation of the unsteady aerodynamic state-space model using a single CFD execution, independent of the number of structural modes. Four different types of inputs are presented that can be used for the simultaneous excitation of the structural modes. Results are presented for a flexible, supersonic semi-span configuration using the CFL3Dv6.4 code.

Portable hyperspectral imager with continuous wave green laser for identification and detection of untreated latent fingerprints on walls.

PubMed

Nakamura, Atsushi; Okuda, Hidekazu; Nagaoka, Takashi; Akiba, Norimitsu; Kurosawa, Kenji; Kuroki, Kenro; Ichikawa, Fumihiko; Torao, Akira; Sota, Takayuki

2015-09-01

Untreated latent fingerprints are known to exhibit fluorescence under UV laser excitation. Previously, the hyperspectral imager (HSI) has been primarily evaluated in terms of its potential to enhance the sensitivity of latent fingerprint detection following treatment by conventional chemical methods in the forensic science field. In this study however, the potential usability of the HSI for the visualization and detection of untreated latent fingerprints by measuring their inherent fluorescence under continuous wave (CW) visible laser excitation was examined. Its potential to undertake spectral separation of overlapped fingerprints was also evaluated. The excitation wavelength dependence of fluorescent images was examined using an untreated palm print on a steel based wall, and it was found that green laser excitation is superior to blue and yellow lasers' excitation for the production of high contrast fluorescence images. In addition, a spectral separation method for overlapped fingerprints/palm prints on a plaster wall was proposed using new images converted by the division and subtraction of two single wavelength images constructed based on measured hyperspectral data (HSD). In practical tests, the relative isolation of two overlapped fingerprints/palm prints was successful in twelve out of seventeen cases. Only one fingerprint/palm print was extracted for an additional three cases. These results revealed that the feasibility of overlapped fingerprint/palm print spectral separation depends on the difference in the temporal degeneration of each fluorescence spectrum. The present results demonstrate that a combination of a portable HSI and CW green laser has considerable potential for the identification and detection of untreated latent fingerprints/palm prints on the walls under study, while the use of HSD makes it practically possible for doubly overlapped fingerprints/palm prints to be separated spectrally. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Imaging B. anthracis heme catabolism in mice using the IFP1.4 gene reporter

NASA Astrophysics Data System (ADS)

Zhu, Banghe; Robinson, Holly; Wilganowski, Nathaniel; Nobles, Christopher L.; Sevick-Muraca, Eva; Maresso, Anthony

2012-03-01

B. anthracis is a gram-positive, spore-forming bacterium which likes all pathogenic bacteria, survive by sequestering heme from its host. To image B. anthracis heme catabolism in vivo, we stably transfect new red excitable fluorescent protein, IFP1.4, that requires the heme catabolism product biliverdin (BV). IFP1.4 reporter has favorable excitation and emission characteristics, which has an absorption peak at 685 nm and an emission peak at 708 nm. Therefore, IFP1.4 reporter can be imaged deeply into the tissue with less contamination from tissue autofluorescence. However, the excitation light "leakage" through optical filters can limit detection and sensitivity of IFP1.4 reporter due to the small Stoke's shift of IFP1.4 fluorescence. To minimize the excitation light leakage, an intensified CCD (ICCD) based infrared fluorescence imaging device was optimized using two band pass filters separated by a focus lens to increase the optical density at the excitation wavelength. In this study, a mouse model (DBA/J2) was first injected with B. anthracis bacteria expressing IFP1.4, 150 μl s.c., on the ventral side of the left thigh. Then mouse was given 250 μl of a 1mM BV solution via I.V. injection. Imaging was conducted as a function of time after infection under light euthanasia, excised tissues were imaged and IFP1.4 fluorescence correlated with standard culture measurements of colony forming units (CFU). The work demonstrates the use of IFP1.4 as a reporter of bacterial utilization of host heme and may provide an important tool for understanding the pathogenesis of bacterial infection and developing new anti-bacterial therapeutics.

Energy levels, radiative rates and electron impact excitation rates for transitions in He-like Ga XXX, Ge XXXI, As XXXII, Se XXXIII and Br XXXIV

NASA Astrophysics Data System (ADS)

Aggarwal, Kanti M.; Keenan, Francis P.

2013-04-01

We report calculations of energy levels, radiative rates and electron impact excitation cross sections and rates for transitions in He-like Ga XXX, Ge XXXI, As XXXII, Se XXXIII and Br XXXIV. The grasp (general-purpose relativistic atomic structure package) is adopted for calculating energy levels and radiative rates. For determining the collision strengths, and subsequently the excitation rates, the Dirac atomic R-matrix code (darc) is used. Oscillator strengths, radiative rates and line strengths are reported for all E1, E2, M1 and M2 transitions among the lowest 49 levels of each ion. Additionally, theoretical lifetimes are provided for all 49 levels of the above five ions. Collision strengths are averaged over a Maxwellian velocity distribution and the effective collision strengths obtained listed over a wide temperature range up to 108 K. Comparisons are made with similar data obtained using the flexible atomic code (fac) to highlight the importance of resonances, included in calculations with darc, in the determination of effective collision strengths. Discrepancies between the collision strengths from darc and fac, particularly for some forbidden transitions, are also discussed. Finally, discrepancies between the present results for effective collision strengths with the darc code and earlier semi-relativistic R-matrix data are noted over a wide range of electron temperatures for many transitions in all ions.

UV Raman imaging--a promising tool for astrobiology: comparative Raman studies with different excitation wavelengths on SNC Martian meteorites.

PubMed

Frosch, Torsten; Tarcea, Nicolae; Schmitt, Michael; Thiele, Hans; Langenhorst, Falko; Popp, Jürgen

2007-02-01

The great capabilities of UV Raman imaging have been demonstrated on the three Martian meteorites: Sayh al Uhaymir, Dar al Gani, and Zagami. Raman spectra without disturbing fluorescence and with high signal-to-noise-ratios and full of spectral features were derived. This result is of utmost importance for the development of powerful instruments for space missions. By point scanning the surfaces of the meteorite samples, it was possible for the first time to construct UV-Raman images out of the array of Raman spectra. Deep-UV Raman images are to the best of our knowledge presented for the first time. The images were used for a discussion of the chemical-mineralogical composition and texture of the meteorite surfaces. Comparative Raman studies applying visible and NIR Raman excitation wavelengths demonstrate a much better performance for UV Raman excitation. This comparative study of different Raman excitation wavelengths at the same sample spots was done by constructing a versatile, robust sample holder with a fixed micro-raster. The overall advantages of UV resonance Raman spectroscopy in terms of sensitivity and selectivity are demonstrated and discussed. Finally the application of this new technique for a UV Raman instrument for envisaged astrobiological focused space missions is suggested.

High Resolution Fluorescence Imaging of Cancers Using Lanthanide Ion-Doped Upconverting Nanocrystals

PubMed Central

Naccache, Rafik; Rodríguez, Emma Martín; Bogdan, Nicoleta; Sanz-Rodríguez, Francisco; de la Cruz, Maria del Carmen Iglesias; de la Fuente, Ángeles Juarranz; Vetrone, Fiorenzo; Jaque, Daniel; Solé, José García; Capobianco, John A.

2012-01-01

During the last decade inorganic luminescent nanoparticles that emit visible light under near infrared (NIR) excitation (in the biological window) have played a relevant role for high resolution imaging of cancer. Indeed, semiconductor quantum dots (QDs) and metal nanoparticles, mostly gold nanorods (GNRs), are already commercially available for this purpose. In this work we review the role which is being played by a relatively new class of nanoparticles, based on lanthanide ion doped nanocrystals, to target and image cancer cells using upconversion fluorescence microscopy. These nanoparticles are insulating nanocrystals that are usually doped with small percentages of two different rare earth (lanthanide) ions: The excited donor ions (usually Yb3+ ion) that absorb the NIR excitation and the acceptor ions (usually Er3+, Ho3+ or Tm3+), that are responsible for the emitted visible (or also near infrared) radiation. The higher conversion efficiency of these nanoparticles in respect to those based on QDs and GNRs, as well as the almost independent excitation/emission properties from the particle size, make them particularly promising for fluorescence imaging. The different approaches of these novel nanoparticles devoted to “in vitro” and “in vivo” cancer imaging, selective targeting and treatment are examined in this review. PMID:24213500

Eliminating Unwanted Far-Field Excitation in Objective-Type TIRF. Part I. Identifying Sources of Nonevanescent Excitation Light

PubMed Central

Brunstein, Maia; Teremetz, Maxime; Hérault, Karine; Tourain, Christophe; Oheim, Martin

2014-01-01

Total internal reflection fluorescence microscopy (TIRFM) achieves subdiffraction axial sectioning by confining fluorophore excitation to a thin layer close to the cell/substrate boundary. However, it is often unknown how thin this light sheet actually is. Particularly in objective-type TIRFM, large deviations from the exponential intensity decay expected for pure evanescence have been reported. Nonevanescent excitation light diminishes the optical sectioning effect, reduces contrast, and renders TIRFM-image quantification uncertain. To identify the sources of this unwanted fluorescence excitation in deeper sample layers, we here combine azimuthal and polar beam scanning (spinning TIRF), atomic force microscopy, and wavefront analysis of beams passing through the objective periphery. Using a variety of intracellular fluorescent labels as well as negative staining experiments to measure cell-induced scattering, we find that azimuthal beam spinning produces TIRFM images that more accurately portray the real fluorophore distribution, but these images are still hampered by far-field excitation. Furthermore, although clearly measureable, cell-induced scattering is not the dominant source of far-field excitation light in objective-type TIRF, at least for most types of weakly scattering cells. It is the microscope illumination optical path that produces a large cell- and beam-angle invariant stray excitation that is insensitive to beam scanning. This instrument-induced glare is produced far from the sample plane, inside the microscope illumination optical path. We identify stray reflections and high-numerical aperture aberrations of the TIRF objective as one important source. This work is accompanied by a companion paper (Pt.2/2). PMID:24606927

Excitation-resolved cone-beam x-ray luminescence tomography.

PubMed

Liu, Xin; Liao, Qimei; Wang, Hongkai; Yan, Zhuangzhi

2015-07-01

Cone-beam x-ray luminescence computed tomography (CB-XLCT), as an emerging imaging technique, plays an important role in in vivo small animal imaging studies. However, CB-XLCT suffers from low-spatial resolution due to the ill-posed nature of reconstruction. We improve the imaging performance of CB-XLCT by using a multiband excitation-resolved imaging scheme combined with principal component analysis. To evaluate the performance of the proposed method, the physical phantom experiment is performed with a custom-made XLCT/XCT imaging system. The experimental results validate the feasibility of the method, where two adjacent nanophosphors (with an edge-to-edge distance of 2.4 mm) can be located.

Multiphoton imaging with high peak power VECSELs

NASA Astrophysics Data System (ADS)

Mirkhanov, Shamil; Quarterman, Adrian H.; Swift, Samuel; Praveen, Bavishna B.; Smyth, Conor J. C.; Wilcox, Keith G.

2016-03-01

Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.

In vivo three-photon microscopy of subcortical structures within an intact mouse brain

NASA Astrophysics Data System (ADS)

Horton, Nicholas G.; Wang, Ke; Kobat, Demirhan; Clark, Catharine G.; Wise, Frank W.; Schaffer, Chris B.; Xu, Chris

2013-03-01

Two-photon fluorescence microscopy enables scientists in various fields including neuroscience, embryology and oncology to visualize in vivo and ex vivo tissue morphology and physiology at a cellular level deep within scattering tissue. However, tissue scattering limits the maximum imaging depth of two-photon fluorescence microscopy to the cortical layer within mouse brain, and imaging subcortical structures currently requires the removal of overlying brain tissue or the insertion of optical probes. Here, we demonstrate non-invasive, high-resolution, in vivo imaging of subcortical structures within an intact mouse brain using three-photon fluorescence microscopy at a spectral excitation window of 1,700 nm. Vascular structures as well as red fluorescent protein-labelled neurons within the mouse hippocampus are imaged. The combination of the long excitation wavelength and the higher-order nonlinear excitation overcomes the limitations of two-photon fluorescence microscopy, enabling biological investigations to take place at a greater depth within tissue.

Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy

PubMed Central

Chang, Chia-Yuan; Cheng, Li-Chung; Su, Hung-Wei; Hu, Yvonne Yuling; Cho, Keng-Chi; Yen, Wei-Chung; Xu, Chris; Dong, Chen Yuan; Chen, Shean-Jen

2014-01-01

Temporal profile distortions reduce excitation efficiency and image quality in temporal focusing-based multiphoton microscopy. In order to compensate the distortions, a wavefront sensorless adaptive optics system (AOS) was integrated into the microscope. The feedback control signal of the AOS was acquired from local image intensity maximization via a hill-climbing algorithm. The control signal was then utilized to drive a deformable mirror in such a way as to eliminate the distortions. With the AOS correction, not only is the axial excitation symmetrically refocused, but the axial resolution with full two-photon excited fluorescence (TPEF) intensity is also maintained. Hence, the contrast of the TPEF image of a R6G-doped PMMA thin film is enhanced along with a 3.7-fold increase in intensity. Furthermore, the TPEF image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved. PMID:24940539

Nanoparticles as multimodal photon transducers of ionizing radiation

NASA Astrophysics Data System (ADS)

Pratt, Edwin C.; Shaffer, Travis M.; Zhang, Qize; Drain, Charles Michael; Grimm, Jan

2018-05-01

In biomedical imaging, nanoparticles combined with radionuclides that generate Cerenkov luminescence are used in diagnostic imaging, photon-induced therapies and as activatable probes. In these applications, the nanoparticle is often viewed as a carrier inert to ionizing radiation from the radionuclide. However, certain phenomena such as enhanced nanoparticle luminescence and generation of reactive oxygen species cannot be completely explained by Cerenkov luminescence interactions with nanoparticles. Herein, we report methods to examine the mechanisms of nanoparticle excitation by radionuclides, including interactions with Cerenkov luminescence, β particles and γ radiation. We demonstrate that β-scintillation contributes appreciably to excitation and reactivity in certain nanoparticle systems, and that excitation by radionuclides of nanoparticles composed of large atomic number atoms generates X-rays, enabling multiplexed imaging through single photon emission computed tomography. These findings demonstrate practical optical imaging and therapy using radionuclides with emission energies below the Cerenkov threshold, thereby expanding the list of applicable radionuclides.

Principle component analysis and linear discriminant analysis of multi-spectral autofluorescence imaging data for differentiating basal cell carcinoma and healthy skin

NASA Astrophysics Data System (ADS)

Chernomyrdin, Nikita V.; Zaytsev, Kirill I.; Lesnichaya, Anastasiya D.; Kudrin, Konstantin G.; Cherkasova, Olga P.; Kurlov, Vladimir N.; Shikunova, Irina A.; Perchik, Alexei V.; Yurchenko, Stanislav O.; Reshetov, Igor V.

2016-09-01

In present paper, an ability to differentiate basal cell carcinoma (BCC) and healthy skin by combining multi-spectral autofluorescence imaging, principle component analysis (PCA), and linear discriminant analysis (LDA) has been demonstrated. For this purpose, the experimental setup, which includes excitation and detection branches, has been assembled. The excitation branch utilizes a mercury arc lamp equipped with a 365-nm narrow-linewidth excitation filter, a beam homogenizer, and a mechanical chopper. The detection branch employs a set of bandpass filters with the central wavelength of spectral transparency of λ = 400, 450, 500, and 550 nm, and a digital camera. The setup has been used to study three samples of freshly excised BCC. PCA and LDA have been implemented to analyze the data of multi-spectral fluorescence imaging. Observed results of this pilot study highlight the advantages of proposed imaging technique for skin cancer diagnosis.

Survey of adaptive image coding techniques

NASA Technical Reports Server (NTRS)

Habibi, A.

1977-01-01

The general problem of image data compression is discussed briefly with attention given to the use of Karhunen-Loeve transforms, suboptimal systems, and block quantization. A survey is then conducted encompassing the four categories of adaptive systems: (1) adaptive transform coding (adaptive sampling, adaptive quantization, etc.), (2) adaptive predictive coding (adaptive delta modulation, adaptive DPCM encoding, etc.), (3) adaptive cluster coding (blob algorithms and the multispectral cluster coding technique), and (4) adaptive entropy coding.

Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein

PubMed Central

Chu, Jun; Haynes, Russell D; Corbel, Stéphane Y; Li, Pengpeng; González-González, Emilio; Burg, John S; Ataie, Niloufar J; Lam, Amy J; Cranfill, Paula J; Baird, Michelle A; Davidson, Michael W; Ng, Ho-Leung; Garcia, K Christopher; Contag, Christopher H; Shen, Kang; Blau, Helen M; Lin, Michael Z

2014-01-01

A method for non-invasive visualization of genetically labelled cells in animal disease models with micron-level resolution would greatly facilitate development of cell-based therapies. Imaging of fluorescent proteins (FPs) using red excitation light in the “optical window” above 600 nm is one potential method for visualizing implanted cells. However, previous efforts to engineer FPs with peak excitation beyond 600 nm have resulted in undesirable reductions in brightness. Here we report three new red-excitable monomeric FPs obtained by structure-guided mutagenesis of mNeptune, previously the brightest monomeric FP when excited beyond 600 nm. Two of these, mNeptune2 and mNeptune2.5, demonstrate improved maturation and brighter fluorescence, while the third, mCardinal, has a red-shifted excitation spectrum without reduction in brightness. We show that mCardinal can be used to non-invasively and longitudinally visualize the differentiation of myoblasts and stem cells into myocytes in living mice with high anatomical detail. PMID:24633408

Portable multispectral imaging system for oral cancer diagnosis

NASA Astrophysics Data System (ADS)

Hsieh, Yao-Fang; Ou-Yang, Mang; Lee, Cheng-Chung

2013-09-01

This study presents the portable multispectral imaging system that can acquire the image of specific spectrum in vivo for oral cancer diagnosis. According to the research literature, the autofluorescence of cells and tissue have been widely applied to diagnose oral cancer. The spectral distribution is difference for lesions of epithelial cells and normal cells after excited fluorescence. We have been developed the hyperspectral and multispectral techniques for oral cancer diagnosis in three generations. This research is the third generation. The excited and emission spectrum for the diagnosis are acquired from the research of first generation. The portable system for detection of oral cancer is modified for existing handheld microscope. The UV LED is used to illuminate the surface of oral cavity and excite the cells to produce fluorescent. The image passes through the central channel and filters out unwanted spectrum by the selection of filter, and focused by the focus lens on the image sensor. Therefore, we can achieve the specific wavelength image via fluorescence reaction. The specificity and sensitivity of the system are 85% and 90%, respectively.

Eddy current-nulled convex optimized diffusion encoding (EN-CODE) for distortion-free diffusion tensor imaging with short echo times.

PubMed

Aliotta, Eric; Moulin, Kévin; Ennis, Daniel B

2018-02-01

To design and evaluate eddy current-nulled convex optimized diffusion encoding (EN-CODE) gradient waveforms for efficient diffusion tensor imaging (DTI) that is free of eddy current-induced image distortions. The EN-CODE framework was used to generate diffusion-encoding waveforms that are eddy current-compensated. The EN-CODE DTI waveform was compared with the existing eddy current-nulled twice refocused spin echo (TRSE) sequence as well as monopolar (MONO) and non-eddy current-compensated CODE in terms of echo time (TE) and image distortions. Comparisons were made in simulations, phantom experiments, and neuro imaging in 10 healthy volunteers. The EN-CODE sequence achieved eddy current compensation with a significantly shorter TE than TRSE (78 versus 96 ms) and a slightly shorter TE than MONO (78 versus 80 ms). Intravoxel signal variance was lower in phantoms with EN-CODE than with MONO (13.6 ± 11.6 versus 37.4 ± 25.8) and not different from TRSE (15.1 ± 11.6), indicating good robustness to eddy current-induced image distortions. Mean fractional anisotropy values in brain edges were also significantly lower with EN-CODE than with MONO (0.16 ± 0.01 versus 0.24 ± 0.02, P < 1 x 10 -5 ) and not different from TRSE (0.16 ± 0.01 versus 0.16 ± 0.01, P = nonsignificant). The EN-CODE sequence eliminated eddy current-induced image distortions in DTI with a TE comparable to MONO and substantially shorter than TRSE. Magn Reson Med 79:663-672, 2018. © 2017 International Society for Magnetic Resonance in Medicine. © 2017 International Society for Magnetic Resonance in Medicine.

Magnetic resonance image compression using scalar-vector quantization

NASA Astrophysics Data System (ADS)

Mohsenian, Nader; Shahri, Homayoun

1995-12-01

A new coding scheme based on the scalar-vector quantizer (SVQ) is developed for compression of medical images. SVQ is a fixed-rate encoder and its rate-distortion performance is close to that of optimal entropy-constrained scalar quantizers (ECSQs) for memoryless sources. The use of a fixed-rate quantizer is expected to eliminate some of the complexity issues of using variable-length scalar quantizers. When transmission of images over noisy channels is considered, our coding scheme does not suffer from error propagation which is typical of coding schemes which use variable-length codes. For a set of magnetic resonance (MR) images, coding results obtained from SVQ and ECSQ at low bit-rates are indistinguishable. Furthermore, our encoded images are perceptually indistinguishable from the original, when displayed on a monitor. This makes our SVQ based coder an attractive compression scheme for picture archiving and communication systems (PACS), currently under consideration for an all digital radiology environment in hospitals, where reliable transmission, storage, and high fidelity reconstruction of images are desired.

Codestream-Based Identification of JPEG 2000 Images with Different Coding Parameters

NASA Astrophysics Data System (ADS)

Watanabe, Osamu; Fukuhara, Takahiro; Kiya, Hitoshi

A method of identifying JPEG 2000 images with different coding parameters, such as code-block sizes, quantization-step sizes, and resolution levels, is presented. It does not produce false-negative matches regardless of different coding parameters (compression rate, code-block size, and discrete wavelet transform (DWT) resolutions levels) or quantization step sizes. This feature is not provided by conventional methods. Moreover, the proposed approach is fast because it uses the number of zero-bit-planes that can be extracted from the JPEG 2000 codestream by only parsing the header information without embedded block coding with optimized truncation (EBCOT) decoding. The experimental results revealed the effectiveness of image identification based on the new method.

Cone-beam x-ray luminescence computed tomography based on x-ray absorption dosage.

PubMed

Liu, Tianshuai; Rong, Junyan; Gao, Peng; Zhang, Wenli; Liu, Wenlei; Zhang, Yuanke; Lu, Hongbing

2018-02-01

With the advances of x-ray excitable nanophosphors, x-ray luminescence computed tomography (XLCT) has become a promising hybrid imaging modality. In particular, a cone-beam XLCT (CB-XLCT) system has demonstrated its potential in in vivo imaging with the advantage of fast imaging speed over other XLCT systems. Currently, the imaging models of most XLCT systems assume that nanophosphors emit light based on the intensity distribution of x-ray within the object, not completely reflecting the nature of the x-ray excitation process. To improve the imaging quality of CB-XLCT, an imaging model that adopts an excitation model of nanophosphors based on x-ray absorption dosage is proposed in this study. To solve the ill-posed inverse problem, a reconstruction algorithm that combines the adaptive Tikhonov regularization method with the imaging model is implemented for CB-XLCT reconstruction. Numerical simulations and phantom experiments indicate that compared with the traditional forward model based on x-ray intensity, the proposed dose-based model could improve the image quality of CB-XLCT significantly in terms of target shape, localization accuracy, and image contrast. In addition, the proposed model behaves better in distinguishing closer targets, demonstrating its advantage in improving spatial resolution. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

BSA Au clusters as a probe for enhanced fluorescence detection using multipulse excitation scheme.

PubMed

Raut, Sangram L; Rich, Ryan; Fudala, Rafal; Kokate, R; Kimball, J D; Borejdo, Julian; Vishwanatha, Jamboor K; Gryczynski, Zygmunt; Gryczynski, Ignacy

2014-01-01

Although BSA Au clusters fluoresce in red region (λmax: 650 nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1 µs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.

Bright long-lived luminescence of silicon nanocrystals sensitized by two-photon absorbing antenna

PubMed Central

Ravotto, Luca; Chen, Qi; Ma, Yuguo; Vinogradov, Sergei A.; Locritani, Mirko; Bergamini, Giacomo; Negri, Fabrizia; Yu, Yixuan; Korgel, Brian A.; Ceroni, Paola

2017-01-01

Summary Silicon nanocrystals of the average diameter of 5 nm, functionalized with 4,7-di(2-thienyl)-2,1,3-benzothiadiazole chromophores (TBT) and dodecyl chains, exhibit near-infrared emission upon one-photon (1P) excitation at 515 nm and two-photon (2P) excitation at 960 nm. By using TBT chromophores as an antenna we were able to enhance both 1P and 2P absorption cross-sections of the silicon nanocrystals to more efficiently excite their long-lived luminescence. These results chart a path to two-photon-excitable imaging probes with long-lived oxygen-independent luminescence - a rare combination of properties that should allow for a substantial increase in imaging contrast. PMID:28966989

Compress compound images in H.264/MPGE-4 AVC by exploiting spatial correlation.

PubMed

Lan, Cuiling; Shi, Guangming; Wu, Feng

2010-04-01

Compound images are a combination of text, graphics and natural image. They present strong anisotropic features, especially on the text and graphics parts. These anisotropic features often render conventional compression inefficient. Thus, this paper proposes a novel coding scheme from the H.264 intraframe coding. In the scheme, two new intramodes are developed to better exploit spatial correlation in compound images. The first is the residual scalar quantization (RSQ) mode, where intrapredicted residues are directly quantized and coded without transform. The second is the base colors and index map (BCIM) mode that can be viewed as an adaptive color quantization. In this mode, an image block is represented by several representative colors, referred to as base colors, and an index map to compress. Every block selects its coding mode from two new modes and the previous intramodes in H.264 by rate-distortion optimization (RDO). Experimental results show that the proposed scheme improves the coding efficiency even more than 10 dB at most bit rates for compound images and keeps a comparable efficient performance to H.264 for natural images.

Toward heterogeneity in feedforward network with synaptic delays based on FitzHugh-Nagumo model

NASA Astrophysics Data System (ADS)

Qin, Ying-Mei; Men, Cong; Zhao, Jia; Han, Chun-Xiao; Che, Yan-Qiu

2018-01-01

We focus on the role of heterogeneity on the propagation of firing patterns in feedforward network (FFN). Effects of heterogeneities both in parameters of neuronal excitability and synaptic delays are investigated systematically. Neuronal heterogeneity is found to modulate firing rates and spiking regularity by changing the excitability of the network. Synaptic delays are strongly related with desynchronized and synchronized firing patterns of the FFN, which indicate that synaptic delays may play a significant role in bridging rate coding and temporal coding. Furthermore, quasi-coherence resonance (quasi-CR) phenomenon is observed in the parameter domain of connection probability and delay-heterogeneity. All these phenomena above enable a detailed characterization of neuronal heterogeneity in FFN, which may play an indispensable role in reproducing the important properties of in vivo experiments.

Multispectral imaging method and apparatus

DOEpatents

Sandison, D.R.; Platzbecker, M.R.; Vargo, T.D.; Lockhart, R.R.; Descour, M.R.; Richards-Kortum, R.

1999-07-06

A multispectral imaging method and apparatus are described which are adapted for use in determining material properties, especially properties characteristic of abnormal non-dermal cells. A target is illuminated with a narrow band light beam. The target expresses light in response to the excitation. The expressed light is collected and the target's response at specific response wavelengths to specific excitation wavelengths is measured. From the measured multispectral response the target's properties can be determined. A sealed, remote probe and robust components can be used for cervical imaging. 5 figs.

Multispectral imaging method and apparatus

DOEpatents

Sandison, David R.; Platzbecker, Mark R.; Vargo, Timothy D.; Lockhart, Randal R.; Descour, Michael R.; Richards-Kortum, Rebecca

1999-01-01

A multispectral imaging method and apparatus adapted for use in determining material properties, especially properties characteristic of abnormal non-dermal cells. A target is illuminated with a narrow band light beam. The target expresses light in response to the excitation. The expressed light is collected and the target's response at specific response wavelengths to specific excitation wavelengths is measured. From the measured multispectral response the target's properties can be determined. A sealed, remote probe and robust components can be used for cervical imaging

Digitally synthesized beat frequency-multiplexed fluorescence lifetime spectroscopy

PubMed Central

Chan, Jacky C. K.; Diebold, Eric D.; Buckley, Brandon W.; Mao, Sien; Akbari, Najva; Jalali, Bahram

2014-01-01

Frequency domain fluorescence lifetime imaging is a powerful technique that enables the observation of subtle changes in the molecular environment of a fluorescent probe. This technique works by measuring the phase delay between the optical emission and excitation of fluorophores as a function of modulation frequency. However, high-resolution measurements are time consuming, as the excitation modulation frequency must be swept, and faster low-resolution measurements at a single frequency are prone to large errors. Here, we present a low cost optical system for applications in real-time confocal lifetime imaging, which measures the phase vs. frequency spectrum without sweeping. Deemed Lifetime Imaging using Frequency-multiplexed Excitation (LIFE), this technique uses a digitally-synthesized radio frequency comb to drive an acousto-optic deflector, operated in a cat’s-eye configuration, to produce a single laser excitation beam modulated at multiple beat frequencies. We demonstrate simultaneous fluorescence lifetime measurements at 10 frequencies over a bandwidth of 48 MHz, enabling high speed frequency domain lifetime analysis of single- and multi-component sample mixtures. PMID:25574449

Time Resolved Near Field Optical Microscopy

NASA Astrophysics Data System (ADS)

Stark, J. B.

1996-03-01

We use broadband pulses to image the carrier dynamics of semiconductor microstructures on a 150 nm spatial scale, with a time resolution of 60 femtoseconds. Etched disks of GaAs/AlGaAs multiple quantum well material, 10 microns in diameter, are excited with a 30 fs pump from a Ti:Sapphire laser, and probed using a near-field optical microscope. The nonlinear transmission of the microdisks is measured using a double-modulation technique, sensitive to transmission changes of 0.0005 within a 150 nm diameter spot on the sample. This spot is scanned to produce an image of the sample. The nonlinear response is produced by the occupation of phase space by the excited distribution. Images of this evolving distribution are collected at time intervals following excitation, measuring the relaxation of carriers at each point in the microdisk. The resulting data can be viewed as a movie of the carrier dynamics of nonequilibrium distributions in excited semiconductor structures. Work done in collaboration with U. Mohideen and R. E. Slusher.

Two-Photon Optical Properties of Near-Infrared Dyes at 1.55 microns Excitation

PubMed Central

Berezin, Mikhail; Zhan, Chun; Lee, Hyeran; Joo, Chulmin; Akers, Walter; Yazdanfar, Siavash; Achilefu, Samuel

2011-01-01

Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross-sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC showed the highest 2P action cross-section (~103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region. PMID:21866928

Excitation-scanning hyperspectral imaging system for microscopic and endoscopic applications

NASA Astrophysics Data System (ADS)

Mayes, Sam A.; Leavesley, Silas J.; Rich, Thomas C.

2016-04-01

Current microscopic and endoscopic technologies for cancer screening utilize white-light illumination sources. Hyper-spectral imaging has been shown to improve sensitivity while retaining specificity when compared to white-light imaging in both microscopy and in vivo imaging. However, hyperspectral imaging methods have historically suffered from slow acquisition times due to the narrow bandwidth of spectral filters. Often minutes are required to gather a full image stack. We have developed a novel approach called excitation-scanning hyperspectral imaging that provides 2-3 orders of magnitude increased signal strength. This reduces acquisition times significantly, allowing for live video acquisition. Here, we describe a preliminary prototype excitation-scanning hyperspectral imaging system that can be coupled with endoscopes or microscopes for hyperspectral imaging of tissues and cells. Our system is comprised of three subsystems: illumination, transmission, and imaging. The illumination subsystem employs light-emitting diode arrays to illuminate at different wavelengths. The transmission subsystem utilizes a unique geometry of optics and a liquid light guide. Software controls allow us to interface with and control the subsystems and components. Digital and analog signals are used to coordinate wavelength intensity, cycling and camera triggering. Testing of the system shows it can cycle 16 wavelengths at as fast as 1 ms per cycle. Additionally, more than 18% of the light transmits through the system. Our setup should allow for hyperspectral imaging of tissue and cells in real time.

Optical image encryption using QR code and multilevel fingerprints in gyrator transform domains

NASA Astrophysics Data System (ADS)

Wei, Yang; Yan, Aimin; Dong, Jiabin; Hu, Zhijuan; Zhang, Jingtao

2017-11-01

A new concept of GT encryption scheme is proposed in this paper. We present a novel optical image encryption method by using quick response (QR) code and multilevel fingerprint keys in gyrator transform (GT) domains. In this method, an original image is firstly transformed into a QR code, which is placed in the input plane of cascaded GTs. Subsequently, the QR code is encrypted into the cipher-text by using multilevel fingerprint keys. The original image can be obtained easily by reading the high-quality retrieved QR code with hand-held devices. The main parameters used as private keys are GTs' rotation angles and multilevel fingerprints. Biometrics and cryptography are integrated with each other to improve data security. Numerical simulations are performed to demonstrate the validity and feasibility of the proposed encryption scheme. In the future, the method of applying QR codes and fingerprints in GT domains possesses much potential for information security.

Reduced field-of-view imaging for single-shot MRI with an amplitude-modulated chirp pulse excitation and Fourier transform reconstruction.

PubMed

Li, Jing; Zhang, Miao; Chen, Lin; Cai, Congbo; Sun, Huijun; Cai, Shuhui

2015-06-01

We employ an amplitude-modulated chirp pulse to selectively excite spins in one or more regions of interest (ROIs) to realize reduced field-of-view (rFOV) imaging based on single-shot spatiotemporally encoded (SPEN) sequence and Fourier transform reconstruction. The proposed rFOV imaging method was theoretically analyzed and illustrated with numerical simulation and tested with phantom experiments and in vivo rat experiments. In addition, point spread function was applied to demonstrate the feasibility of the proposed method. To evaluate the proposed method, the rFOV results were compared with those obtained using the EPI method with orthogonal RF excitation. The simulation and experimental results show that the proposed method can image one or two separated ROIs along the SPEN dimension in a single shot with higher spatial resolution, less sensitive to field inhomogeneity, and practically no aliasing artifacts. In addition, the proposed method may produce rFOV images with comparable signal-to-noise ratio to the rFOV EPI images. The proposed method is promising for the applications under severe susceptibility heterogeneities and for imaging separate ROIs simultaneously. Copyright © 2015 Elsevier Inc. All rights reserved.

Radiation pressure excitation of Low Temperature Atomic Force & Magnetic Force Microscope (LT-AFM/MFM) for Imaging

NASA Astrophysics Data System (ADS)

Karci, Ozgur; Celik, Umit; Oral, Ahmet; NanoMagnetics Instruments Ltd. Team; Middle East Tech Univ Team

2015-03-01

We describe a novel method for excitation of Atomic Force Microscope (AFM) cantilevers by means of radiation pressure for imaging in an AFM for the first time. Piezo excitation is the most common method for cantilever excitation, but it may cause spurious resonance peaks. A fiber optic interferometer with 1310 nm laser was used both to measure the deflection of cantilever and apply a force to the cantilever in a LT-AFM/MFM from NanoMagnetics Instruments. The laser power was modulated at the cantilever`s resonance frequency by a digital Phase Lock Loop (PLL). The force exerted by the radiation pressure on a perfectly reflecting surface by a laser beam of power P is F = 2P/c. We typically modulate the laser beam by ~ 800 μW and obtain 10nm oscillation amplitude with Q ~ 8,000 at 2.5x10-4 mbar. The cantilever's stiffness can be accurately calibrated by using the radiation pressure. We have demonstrated performance of the radiation pressure excitation in AFM/MFM by imaging a hard disk sample between 4-300K and Abrikosov vortex lattice in BSCCO single crystal at 4K to for the first time.

Transmission and storage of medical images with patient information.

PubMed

Acharya U, Rajendra; Subbanna Bhat, P; Kumar, Sathish; Min, Lim Choo

2003-07-01

Digital watermarking is a technique of hiding specific identification data for copyright authentication. This technique is adapted here for interleaving patient information with medical images, to reduce storage and transmission overheads. The text data is encrypted before interleaving with images to ensure greater security. The graphical signals are interleaved with the image. Two types of error control-coding techniques are proposed to enhance reliability of transmission and storage of medical images interleaved with patient information. Transmission and storage scenarios are simulated with and without error control coding and a qualitative as well as quantitative interpretation of the reliability enhancement resulting from the use of various commonly used error control codes such as repetitive, and (7,4) Hamming code is provided.

Design and implementation of a scene-dependent dynamically selfadaptable wavefront coding imaging system

NASA Astrophysics Data System (ADS)

Carles, Guillem; Ferran, Carme; Carnicer, Artur; Bosch, Salvador

2012-01-01

A computational imaging system based on wavefront coding is presented. Wavefront coding provides an extension of the depth-of-field at the expense of a slight reduction of image quality. This trade-off results from the amount of coding used. By using spatial light modulators, a flexible coding is achieved which permits it to be increased or decreased as needed. In this paper a computational method is proposed for evaluating the output of a wavefront coding imaging system equipped with a spatial light modulator, with the aim of thus making it possible to implement the most suitable coding strength for a given scene. This is achieved in an unsupervised manner, thus the whole system acts as a dynamically selfadaptable imaging system. The program presented here controls the spatial light modulator and the camera, and also processes the images in a synchronised way in order to implement the dynamic system in real time. A prototype of the system was implemented in the laboratory and illustrative examples of the performance are reported in this paper. Program summaryProgram title: DynWFC (Dynamic WaveFront Coding) Catalogue identifier: AEKC_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEKC_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 10 483 No. of bytes in distributed program, including test data, etc.: 2 437 713 Distribution format: tar.gz Programming language: Labview 8.5 and NI Vision and MinGW C Compiler Computer: Tested on PC Intel ® Pentium ® Operating system: Tested on Windows XP Classification: 18 Nature of problem: The program implements an enhanced wavefront coding imaging system able to adapt the degree of coding to the requirements of a specific scene. The program controls the acquisition by a camera, the display of a spatial light modulator and the image processing operations synchronously. The spatial light modulator is used to implement the phase mask with flexibility given the trade-off between depth-of-field extension and image quality achieved. The action of the program is to evaluate the depth-of-field requirements of the specific scene and subsequently control the coding established by the spatial light modulator, in real time.

Imaging and characterizing shear wave and shear modulus under orthogonal acoustic radiation force excitation using OCT Doppler variance method.

PubMed

Zhu, Jiang; Qu, Yueqiao; Ma, Teng; Li, Rui; Du, Yongzhao; Huang, Shenghai; Shung, K Kirk; Zhou, Qifa; Chen, Zhongping

2015-05-01

We report on a novel acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE) technique for imaging shear wave and quantifying shear modulus under orthogonal acoustic radiation force (ARF) excitation using the optical coherence tomography (OCT) Doppler variance method. The ARF perpendicular to the OCT beam is produced by a remote ultrasonic transducer. A shear wave induced by ARF excitation propagates parallel to the OCT beam. The OCT Doppler variance method, which is sensitive to the transverse vibration, is used to measure the ARF-induced vibration. For analysis of the shear modulus, the Doppler variance method is utilized to visualize shear wave propagation instead of Doppler OCT method, and the propagation velocity of the shear wave is measured at different depths of one location with the M scan. In order to quantify shear modulus beyond the OCT imaging depth, we move ARF to a deeper layer at a known step and measure the time delay of the shear wave propagating to the same OCT imaging depth. We also quantitatively map the shear modulus of a cross-section in a tissue-equivalent phantom after employing the B scan.

A program to compute the two-step excitation of mesospheric sodium atoms for the Polychromatic Laser Guide Star Project

NASA Astrophysics Data System (ADS)

Bellanger, Véronique; Courcelle, Arnaud; Petit, Alain

2004-09-01

A program to compute the two-step excitation of sodium atoms ( 3S→3P→4D) using the density-matrix formalism is presented. The BEACON program calculates population evolution and the number of photons emitted by fluorescence from the 3P, 4D, 4P, 4S levels. Program summaryTitle of program: BEACON Catalogue identifier:ADSX Program Summary URL:http://cpc.cs.qub.ac.uk/cpc/summaries/ADSX Program obtainable from: CPC Program Library, Queen's University of Belfast, N. Ireland Licensing provisions: none Operating systems under which the program has been tested: Win; Unix Programming language used: FORTRAN 77 Memory required to execute with typical data: 1 Mw Number of bits in a word: 32 Number of processors used: 1 (a parallel version of this code is also available and can be obtained on request) Number of lines in distributed program, including test data, etc.: 29 287 Number of bytes in distributed program, including test data, etc.: 830 331 Distribution format: tar.gz CPC Program Library subprograms used: none Nature of physical problem: Resolution of the Bloch equations in the case of the two-step laser excitation of sodium atoms. Method of solution: The program BEACON calculates the evolution of level population versus time using the density-matrix formalism. The number of photons emitted from the 3P, 4D and 4P levels is calculated using the branching ratios and the level lifetimes. Restriction on the complexity of the problem: Since the backscatter emission is calculated after the excitation process, excitation with laser pulse duration longer than the 4D level lifetime cannot be rigorously treated. Particularly, cw laser excitation cannot be calculated with this code. Typical running time:12 h

On- and off-axis spectral emission features from laser-produced gas breakdown plasmas

NASA Astrophysics Data System (ADS)

Harilal, S. S.; Skrodzki, P. J.; Miloshevsky, A.; Brumfield, B. E.; Phillips, M. C.; Miloshevsky, G.

2017-06-01

Laser-heated gas breakdown plasmas or sparks emit profoundly in the ultraviolet and visible region of the electromagnetic spectrum with contributions from ionic, atomic, and molecular species. Laser created kernels expand into a cold ambient with high velocities during their early lifetime followed by confinement of the plasma kernel and eventually collapse. However, the plasma kernels produced during laser breakdown of gases are also capable of exciting and ionizing the surrounding ambient medium. Two mechanisms can be responsible for excitation and ionization of the surrounding ambient: photoexcitation and ionization by intense ultraviolet emission from the sparks produced during the early times of their creation and/or heating by strong shocks generated by the kernel during its expansion into the ambient. In this study, an investigation is made on the spectral features of on- and off-axis emission of laser-induced plasma breakdown kernels generated in atmospheric pressure conditions with an aim to elucidate the mechanisms leading to ambient excitation and emission. Pulses from an Nd:YAG laser emitting at 1064 nm with a pulse duration of 6 ns are used to generate plasma kernels. Laser sparks were generated in air, argon, and helium gases to provide different physical properties of expansion dynamics and plasma chemistry considering the differences in laser absorption properties, mass density, and speciation. Point shadowgraphy and time-resolved imaging were used to evaluate the shock wave and spark self-emission morphology at early and late times, while space and time resolved spectroscopy is used for evaluating the emission features and for inferring plasma physical conditions at on- and off-axis positions. The structure and dynamics of the plasma kernel obtained using imaging techniques are also compared to numerical simulations using the computational fluid dynamics code. The emission from the kernel showed that spectral features from ions, atoms, and molecules are separated in time with early time temperatures and densities in excess of 35 000 K and 4 × 1018/cm3 with an existence of thermal equilibrium. However, the emission from the off-kernel positions from the breakdown plasmas showed enhanced ultraviolet radiation with the presence of N2 bands and is represented by non-local thermodynamic equilibrium (non-LTE) conditions. Our results also highlight that the ultraviolet radiation emitted during the early time of spark evolution is the predominant source of the photo-excitation of the surrounding medium.

On- and off-axis spectral emission features from laser-produced gas breakdown plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harilal, S. S.; Skrodzki, P. J.; Miloshevsky, A.

Laser-heated gas breakdown plasmas or sparks emit profoundly in the ultraviolet and visible region of the electromagnetic spectrum with contributions from ionic, atomic, and molecular species. Laser created kernels expand into a cold ambient with high velocities during its early lifetime followed by confinement of the plasma kernel and eventually collapse. However, the plasma kernels produced during laser breakdown of gases are also capable of exciting and ionizing the surrounding ambient medium. Two mechanisms can be responsible for excitation and ionization of surrounding ambient: viz. photoexcitation and ionization by intense ultraviolet emission from the sparks produced during the early timesmore » of its creation and/or heating by strong shocks generated by the kernel during its expansion into the ambient. In this study, an investigation is made on the spectral features of on- and off-axis emission features of laser-induced plasma breakdown kernels generated in atmospheric pressure conditions with an aim to elucidate the mechanisms leading to ambient excitation and emission. Pulses from an Nd:YAG laser emitting at 1064 nm with 6 ns pulse duration are used to generate plasma kernels. Laser sparks were generated in air, argon, and helium gases to provide different physical properties of expansion dynamics and plasma chemistry considering the differences in laser absorption properties, mass density and speciation. Point shadowgraphy and time-resolved imaging were used to evaluate the shock wave and spark self-emission morphology at early and late times while space and time resolved spectroscopy is used for evaluating the emission features as well as for inferring plasma fundaments at on- and off-axis. Structure and dynamics of the plasma kernel obtained using imaging techniques are also compared to numerical simulations using computational fluid dynamics code. The emission from the kernel showed that spectral features from ions, atoms and molecules are separated in time with an early time temperatures and densities in excess of 35000 K and 4×10 18 /cm 3 with an existence of thermal equilibrium. However, the emission from the off-kernel positions from the breakdown plasmas showed enhanced ultraviolet radiation with the presence of N 2 bands and represented by non-LTE conditions. Finally, our results also highlight that the ultraviolet radiation emitted during early time of spark evolution is the predominant source of the photo-excitation of the surrounding medium.« less

On- and off-axis spectral emission features from laser-produced gas breakdown plasmas

DOE PAGES

Harilal, S. S.; Skrodzki, P. J.; Miloshevsky, A.; ...

2017-06-01

Laser-heated gas breakdown plasmas or sparks emit profoundly in the ultraviolet and visible region of the electromagnetic spectrum with contributions from ionic, atomic, and molecular species. Laser created kernels expand into a cold ambient with high velocities during its early lifetime followed by confinement of the plasma kernel and eventually collapse. However, the plasma kernels produced during laser breakdown of gases are also capable of exciting and ionizing the surrounding ambient medium. Two mechanisms can be responsible for excitation and ionization of surrounding ambient: viz. photoexcitation and ionization by intense ultraviolet emission from the sparks produced during the early timesmore » of its creation and/or heating by strong shocks generated by the kernel during its expansion into the ambient. In this study, an investigation is made on the spectral features of on- and off-axis emission features of laser-induced plasma breakdown kernels generated in atmospheric pressure conditions with an aim to elucidate the mechanisms leading to ambient excitation and emission. Pulses from an Nd:YAG laser emitting at 1064 nm with 6 ns pulse duration are used to generate plasma kernels. Laser sparks were generated in air, argon, and helium gases to provide different physical properties of expansion dynamics and plasma chemistry considering the differences in laser absorption properties, mass density and speciation. Point shadowgraphy and time-resolved imaging were used to evaluate the shock wave and spark self-emission morphology at early and late times while space and time resolved spectroscopy is used for evaluating the emission features as well as for inferring plasma fundaments at on- and off-axis. Structure and dynamics of the plasma kernel obtained using imaging techniques are also compared to numerical simulations using computational fluid dynamics code. The emission from the kernel showed that spectral features from ions, atoms and molecules are separated in time with an early time temperatures and densities in excess of 35000 K and 4×1018 /cm3 with an existence of thermal equilibrium. However, the emission from the off-kernel positions from the breakdown plasmas showed enhanced ultraviolet radiation with the presence of N2 bands and represented by non-LTE conditions. Our results also highlight that the ultraviolet radiation emitted during early time of spark evolution is the predominant source of the photo-excitation of the surrounding medium.« less

Quantum image coding with a reference-frame-independent scheme

NASA Astrophysics Data System (ADS)

Chapeau-Blondeau, François; Belin, Etienne

2016-07-01

For binary images, or bit planes of non-binary images, we investigate the possibility of a quantum coding decodable by a receiver in the absence of reference frames shared with the emitter. Direct image coding with one qubit per pixel and non-aligned frames leads to decoding errors equivalent to a quantum bit-flip noise increasing with the misalignment. We show the feasibility of frame-invariant coding by using for each pixel a qubit pair prepared in one of two controlled entangled states. With just one common axis shared between the emitter and receiver, exact decoding for each pixel can be obtained by means of two two-outcome projective measurements operating separately on each qubit of the pair. With strictly no alignment information between the emitter and receiver, exact decoding can be obtained by means of a two-outcome projective measurement operating jointly on the qubit pair. In addition, the frame-invariant coding is shown much more resistant to quantum bit-flip noise compared to the direct non-invariant coding. For a cost per pixel of two (entangled) qubits instead of one, complete frame-invariant image coding and enhanced noise resistance are thus obtained.

Energy levels, oscillator strengths, and transition probabilities for sulfur-like scandium, Sc VI

NASA Astrophysics Data System (ADS)

El-Maaref, A. A.; Abou Halaka, M. M.; Saddeek, Yasser B.

2017-09-01

Energy levels, Oscillator strengths, and transition probabilities for sulfur-like scandium are calculated using CIV3 code. The calculations have been executed in an intermediate coupling scheme using Breit-Pauli Hamiltonian. The present calculations have been compared with the experimental data and other theoretical calculations. LANL code has been used to confirm the accuracy of the present calculations, where the calculations using CIV3 code agree well with the corresponding values by LANL code. The calculated energy levels and oscillator strengths are in reasonable agreement with the published experimental data and theoretical values. We have calculated lifetimes of some excited levels, as well.

Exoplanet Yield Estimation for Decadal Study Concepts using EXOSIMS

NASA Astrophysics Data System (ADS)

Morgan, Rhonda; Lowrance, Patrick; Savransky, Dmitry; Garrett, Daniel

2016-01-01

The anticipated upcoming large mission study concepts for the direct imaging of exo-earths present an exciting opportunity for exoplanet discovery and characterization. While these telescope concepts would also be capable of conducting a broad range of astrophysical investigations, the most difficult technology challenges are driven by the requirements for imaging exo-earths. The exoplanet science yield for these mission concepts will drive design trades and mission concept comparisons.To assist in these trade studies, the Exoplanet Exploration Program Office (ExEP) is developing a yield estimation tool that emphasizes transparency and consistent comparison of various design concepts. The tool will provide a parametric estimate of science yield of various mission concepts using contrast curves from physics-based model codes and Monte Carlo simulations of design reference missions using realistic constraints, such as solar avoidance angles, the observatory orbit, propulsion limitations of star shades, the accessibility of candidate targets, local and background zodiacal light levels, and background confusion by stars and galaxies. The python tool utilizes Dmitry Savransky's EXOSIMS (Exoplanet Open-Source Imaging Mission Simulator) design reference mission simulator that is being developed for the WFIRST Preliminary Science program. ExEP is extending and validating the tool for future mission concepts under consideration for the upcoming 2020 decadal review. We present a validation plan and preliminary yield results for a point design.

Thermosensory processing in the Drosophila brain

PubMed Central

Liu, Wendy W.; Mazor, Ofer; Wilson, Rachel I.

2014-01-01

In Drosophila, just as in vertebrates, changes in external temperature are encoded by bidirectional opponent thermoreceptor cells: some cells are excited by warming and inhibited by cooling, whereas others are excited by cooling and inhibited by warming1,2. The central circuits that process these signals are not understood. In Drosophila, a specific brain region receives input from thermoreceptor cells2,3. Here we show that distinct genetically-identified projection neurons (PNs) in this brain region are excited by cooling, warming, or both. The PNs excited by cooling receive mainly feedforward excitation from cool thermoreceptors. In contrast, the PNs excited by warming (“warm-PNs”) receive both excitation from warm thermoreceptors and crossover inhibition from cool thermoreceptors via inhibitory interneurons. Notably, this crossover inhibition elicits warming-evoked excitation, because warming suppresses tonic activity in cool thermoreceptors. This in turn disinhibits warm-PNs and sums with feedforward excitation evoked by warming. Crossover inhibition could cancel non-thermal activity (noise) that is positively-correlated among warm and cool thermoreceptor cells, while reinforcing thermal activity which is anti-correlated. Our results show how central circuits can combine signals from bidirectional opponent neurons to construct sensitive and robust neural codes. PMID:25739502

ESTEST: A Framework for the Verification and Validation of Electronic Structure Codes

NASA Astrophysics Data System (ADS)

Yuan, Gary; Gygi, Francois

2011-03-01

ESTEST is a verification and validation (V& V) framework for electronic structure codes that supports Qbox, Quantum Espresso, ABINIT, the Exciting Code and plans support for many more. We discuss various approaches to the electronic structure V& V problem implemented in ESTEST, that are related to parsing, formats, data management, search, comparison and analyses. Additionally, an early experiment in the distribution of V& V ESTEST servers among the electronic structure community will be presented. Supported by NSF-OCI 0749217 and DOE FC02-06ER25777.

Optimal fluorescence waveband determination for detecting defect cherry tomatoes using fluorescence excitation-emission matrix

USDA-ARS?s Scientific Manuscript database

A multi-spectral fluorescence imaging technique was used to detect defect cherry tomatoes. The fluorescence excitation and emission matrix was used to measure for defects, sound surface, and stem areas to determine the optimal fluorescence excitation and emission wavelengths for discrimination. Two-...

A fractional Fourier transform analysis of the scattering of ultrasonic waves

PubMed Central

Tant, Katherine M.M.; Mulholland, Anthony J.; Langer, Matthias; Gachagan, Anthony

2015-01-01

Many safety critical structures, such as those found in nuclear plants, oil pipelines and in the aerospace industry, rely on key components that are constructed from heterogeneous materials. Ultrasonic non-destructive testing (NDT) uses high-frequency mechanical waves to inspect these parts, ensuring they operate reliably without compromising their integrity. It is possible to employ mathematical models to develop a deeper understanding of the acquired ultrasonic data and enhance defect imaging algorithms. In this paper, a model for the scattering of ultrasonic waves by a crack is derived in the time–frequency domain. The fractional Fourier transform (FrFT) is applied to an inhomogeneous wave equation where the forcing function is prescribed as a linear chirp, modulated by a Gaussian envelope. The homogeneous solution is found via the Born approximation which encapsulates information regarding the flaw geometry. The inhomogeneous solution is obtained via the inverse Fourier transform of a Gaussian-windowed linear chirp excitation. It is observed that, although the scattering profile of the flaw does not change, it is amplified. Thus, the theory demonstrates the enhanced signal-to-noise ratio permitted by the use of coded excitation, as well as establishing a time–frequency domain framework to assist in flaw identification and classification. PMID:25792967

Multi-channel medical imaging system

DOEpatents

Frangioni, John V

2013-12-31

A medical imaging system provides simultaneous rendering of visible light and fluorescent images. The system may employ dyes in a small-molecule form that remain in the subject's blood stream for several minutes, allowing real-time imaging of the subject's circulatory system superimposed upon a conventional, visible light image of the subject. The system may provide an excitation light source to excite the fluorescent substance and a visible light source for general illumination within the same optical guide used to capture images. The system may be configured for use in open surgical procedures by providing an operating area that is closed to ambient light. The systems described herein provide two or more diagnostic imaging channels for capture of multiple, concurrent diagnostic images and may be used where a visible light image may be usefully supplemented by two or more images that are independently marked for functional interest.

Multi-channel medical imaging system

DOEpatents

Frangioni, John V.

2016-05-03

A medical imaging system provides simultaneous rendering of visible light and fluorescent images. The system may employ dyes in a small-molecule form that remain in a subject's blood stream for several minutes, allowing real-time imaging of the subject's circulatory system superimposed upon a conventional, visible light image of the subject. The system may provide an excitation light source to excite the fluorescent substance and a visible light source for general illumination within the same optical guide used to capture images. The system may be configured for use in open surgical procedures by providing an operating area that is closed to ambient light. The systems described herein provide two or more diagnostic imaging channels for capture of multiple, concurrent diagnostic images and may be used where a visible light image may be usefully supplemented by two or more images that are independently marked for functional interest.

Computing Challenges in Coded Mask Imaging

NASA Technical Reports Server (NTRS)

Skinner, Gerald

2009-01-01

This slide presaentation reviews the complications and challenges in developing computer systems for Coded Mask Imaging telescopes. The coded mask technique is used when there is no other way to create the telescope, (i.e., when there are wide fields of view, high energies for focusing or low energies for the Compton/Tracker Techniques and very good angular resolution.) The coded mask telescope is described, and the mask is reviewed. The coded Masks for the INTErnational Gamma-Ray Astrophysics Laboratory (INTEGRAL) instruments are shown, and a chart showing the types of position sensitive detectors used for the coded mask telescopes is also reviewed. Slides describe the mechanism of recovering an image from the masked pattern. The correlation with the mask pattern is described. The Matrix approach is reviewed, and other approaches to image reconstruction are described. Included in the presentation is a review of the Energetic X-ray Imaging Survey Telescope (EXIST) / High Energy Telescope (HET), with information about the mission, the operation of the telescope, comparison of the EXIST/HET with the SWIFT/BAT and details of the design of the EXIST/HET.

Facile and eco-friendly synthesis of green fluorescent carbon nanodots for applications in bioimaging, patterning and staining

NASA Astrophysics Data System (ADS)

Shi, Lihong; Li, Yanyan; Li, Xiaofeng; Wen, Xiangping; Zhang, Guomei; Yang, Jun; Dong, Chuan; Shuang, Shaomin

2015-04-01

We report a facile and eco-friendly strategy for the fabrication of green fluorescent carbon nanodots (CDs), and demonstrate their applications for bio-imaging, patterning, and staining. A one-pot hydrothermal method using various plant petals yields bright green-emitting CDs, providing an easy way for the production of green fluorescent CDs without the need for a tedious synthetic methodology or the use of toxic/expensive solvents and starting materials. The as-prepared CDs show small size distribution and excellent dispersibility. Their strong green fluorescence is observed when the excitation wavelength is between 430 nm and 490 nm. Moreover, they exhibit high tolerance to various external conditions, such as pH values, external cations, and continuous excitation. Due to minimum toxicity as well as good photoluminescence properties, these CDs can be applied to in vitro and in vivo imaging, patterning, and staining. According to confocal fluorescence imaging of human uterine cervical squamous cell carcinoma cells, CDs penetrate into the cell and enter the cytoplasm and the nucleus. More strikingly, carp is directly fed with CDs for in vivo imaging and shows bright green fluorescence at an excitation wavelength of 470 nm. In addition, the obtained CDs are used as fluorescent inks for drawing luminescence patterns. Finally, we also apply the CDs as a fluorescent dye. Interestingly, the absorbent filter paper with staining emits dramatic fluorescence under 470 nm excitation.We report a facile and eco-friendly strategy for the fabrication of green fluorescent carbon nanodots (CDs), and demonstrate their applications for bio-imaging, patterning, and staining. A one-pot hydrothermal method using various plant petals yields bright green-emitting CDs, providing an easy way for the production of green fluorescent CDs without the need for a tedious synthetic methodology or the use of toxic/expensive solvents and starting materials. The as-prepared CDs show small size distribution and excellent dispersibility. Their strong green fluorescence is observed when the excitation wavelength is between 430 nm and 490 nm. Moreover, they exhibit high tolerance to various external conditions, such as pH values, external cations, and continuous excitation. Due to minimum toxicity as well as good photoluminescence properties, these CDs can be applied to in vitro and in vivo imaging, patterning, and staining. According to confocal fluorescence imaging of human uterine cervical squamous cell carcinoma cells, CDs penetrate into the cell and enter the cytoplasm and the nucleus. More strikingly, carp is directly fed with CDs for in vivo imaging and shows bright green fluorescence at an excitation wavelength of 470 nm. In addition, the obtained CDs are used as fluorescent inks for drawing luminescence patterns. Finally, we also apply the CDs as a fluorescent dye. Interestingly, the absorbent filter paper with staining emits dramatic fluorescence under 470 nm excitation. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr00783f

Fast and background-free three-dimensional (3D) live-cell imaging with lanthanide-doped upconverting nanoparticles.

PubMed

Jo, Hong Li; Song, Yo Han; Park, Jinho; Jo, Eun-Jung; Goh, Yeongchang; Shin, Kyujin; Kim, Min-Gon; Lee, Kang Taek

2015-12-14

We report on the development of a three-dimensional (3D) live-cell imaging technique with high spatiotemporal resolution using lanthanide-doped upconverting nanoparticles (UCNPs). It employs the sectioning capability of confocal microscopy except that the two-dimensional (2D) section images are acquired by wide-field epi-fluorescence microscopy. Although epi-fluorescence images are contaminated with the out-of-focus background in general, the near-infrared (NIR) excitation used for the excitation of UCNPs does not generate any autofluorescence, which helps to lower the background. Moreover, the image blurring due to defocusing was naturally eliminated in the image reconstruction process. The 3D images were used to investigate the cellular dynamics such as nuclear uptake and single-particle tracking that require 3D description.

Demystifying autofluorescence with excitation scanning hyperspectral imaging

NASA Astrophysics Data System (ADS)

Deal, Joshua; Harris, Bradley; Martin, Will; Lall, Malvika; Lopez, Carmen; Rider, Paul; Boudreaux, Carole; Rich, Thomas; Leavesley, Silas J.

2018-02-01

Autofluorescence has historically been considered a nuisance in medical imaging. Many endogenous fluorophores, specifically, collagen, elastin, NADH, and FAD, are found throughout the human body. Diagnostically, these signals can be prohibitive since they can outcompete signals introduced for diagnostic purposes. Recent advances in hyperspectral imaging have allowed the acquisition of significantly more data in a shorter time period by scanning the excitation spectra of fluorophores. The reduced acquisition time and increased signal-to-noise ratio allow for separation of significantly more fluorophores than previously possible. Here, we propose to utilize excitation-scanning of autofluorescence to examine tissues and diagnose pathologies. Spectra of autofluorescent molecules were obtained using a custom inverted microscope (TE-2000, Nikon Instruments) with a Xe arc lamp and thin film tunable filter array (VersaChrome, Semrock, Inc.) Scans utilized excitation wavelengths from 360 nm to 550 nm in 5 nm increments. The resultant spectra were used to examine hyperspectral image stacks from various collaborative studies, including an atherosclerotic rat model and a colon cancer study. Hyperspectral images were analyzed with ENVI and custom Matlab scripts including linear spectral unmixing (LSU) and principal component analysis (PCA). Initial results suggest the ability to separate the signals of endogenous fluorophores and measure the relative concentrations of fluorophores among healthy and diseased states of similar tissues. These results suggest pathology-specific changes to endogenous fluorophores can be detected using excitationscanning hyperspectral imaging. Future work will expand the library of pure molecules and will examine more defined disease states.

Ex vivo catheter-based imaging of coronary atherosclerosis using multimodality OCT and NIRAF excited at 633 nm

PubMed Central

Wang, Hao; Gardecki, Joseph A.; Ughi, Giovanni J.; Jacques, Paulino Vacas; Hamidi, Ehsan; Tearney, Guillermo J.

2015-01-01

While optical coherence tomography (OCT) has been shown to be capable of imaging coronary plaque microstructure, additional chemical/molecular information may be needed in order to determine which lesions are at risk of causing an acute coronary event. In this study, we used a recently developed imaging system and double-clad fiber (DCF) catheter capable of simultaneously acquiring both OCT and red excited near-infrared autofluorescence (NIRAF) images (excitation: 633 nm, emission: 680nm to 900nm). We found that NIRAF is elevated in lesions that contain necrotic core – a feature that is critical for vulnerable plaque diagnosis and that is not readily discriminated by OCT alone. We first utilized a DCF ball lens probe and a bench top setup to acquire en face NIRAF images of aortic plaques ex vivo (n = 20). In addition, we used the OCT-NIRAF system and fully assembled catheters to acquire multimodality images from human coronary arteries (n = 15) prosected from human cadaver hearts (n = 5). Comparison of these images with corresponding histology demonstrated that necrotic core plaques exhibited significantly higher NIRAF intensity than other plaque types. These results suggest that multimodality intracoronary OCT-NIRAF imaging technology may be used in the future to provide improved characterization of coronary artery disease in human patients. PMID:25909020

Modulated Excitation Imaging System for Intravascular Ultrasound.

PubMed

Qiu, Weibao; Wang, Xingying; Chen, Yan; Fu, Qiang; Su, Min; Zhang, Lining; Xia, Jingjing; Dai, Jiyan; Zhang, Yaonan; Zheng, Hairong

2017-08-01

Advances in methodologies and tools often lead to new insights into cardiovascular diseases. Intravascular ultrasound (IVUS) is a well-established diagnostic method that provides high-resolution images of the vessel wall and atherosclerotic plaques. High-frequency (>50 MHz) ultrasound enables the spatial resolution of IVUS to approach that of optical imaging methods. However, the penetration depth decreases when using higher imaging frequencies due to the greater acoustic attenuation. An imaging method that improves the penetration depth of high-resolution IVUS would, therefore, be of major clinical importance. Modulated excitation imaging is known to allow ultrasound waves to penetrate further. This paper presents an ultrasound system specifically for modulated-excitation-based IVUS imaging. The system incorporates a high-voltage waveform generator and an image processing board that are optimized for IVUS applications. In addition, a miniaturized ultrasound transducer has been constructed using a Pb(Mg 1/3 Nb 2/3 )O 3 -PbTiO 3 single crystal to improve the ultrasound characteristics. The results show that the proposed system was able to provide increases of 86.7% in penetration depth and 9.6 dB in the signal-to-noise ratio for 60 MHz IVUS. In vitro tissue samples were also investigated to demonstrate the performance of the system.

Medical image classification based on multi-scale non-negative sparse coding.

PubMed

Zhang, Ruijie; Shen, Jian; Wei, Fushan; Li, Xiong; Sangaiah, Arun Kumar

2017-11-01

With the rapid development of modern medical imaging technology, medical image classification has become more and more important in medical diagnosis and clinical practice. Conventional medical image classification algorithms usually neglect the semantic gap problem between low-level features and high-level image semantic, which will largely degrade the classification performance. To solve this problem, we propose a multi-scale non-negative sparse coding based medical image classification algorithm. Firstly, Medical images are decomposed into multiple scale layers, thus diverse visual details can be extracted from different scale layers. Secondly, for each scale layer, the non-negative sparse coding model with fisher discriminative analysis is constructed to obtain the discriminative sparse representation of medical images. Then, the obtained multi-scale non-negative sparse coding features are combined to form a multi-scale feature histogram as the final representation for a medical image. Finally, SVM classifier is combined to conduct medical image classification. The experimental results demonstrate that our proposed algorithm can effectively utilize multi-scale and contextual spatial information of medical images, reduce the semantic gap in a large degree and improve medical image classification performance. Copyright © 2017 Elsevier B.V. All rights reserved.

Fractonic line excitations: An inroad from three-dimensional elasticity theory

NASA Astrophysics Data System (ADS)

Pai, Shriya; Pretko, Michael

2018-06-01

We demonstrate the existence of a fundamentally new type of excitation, fractonic lines, which are linelike excitations with the restricted mobility properties of fractons. These excitations, described using an amalgamation of higher-form gauge theories with symmetric tensor gauge theories, see direct physical realization as the topological lattice defects of ordinary three-dimensional quantum crystals. Starting with the more familiar elasticity theory, we show how theory maps onto a rank-4 tensor gauge theory, with phonons corresponding to gapless gauge modes and disclination defects corresponding to linelike charges. We derive flux conservation laws which lock these linelike excitations in place, analogous to the higher moment charge conservation laws of fracton theories. This way of encoding mobility restrictions of lattice defects could shed light on melting transitions in three dimensions. This new type of extended object may also be a useful tool in the search for improved quantum error-correcting codes in three dimensions.

Coherent nonlinear optical imaging: beyond fluorescence microscopy.

PubMed

Min, Wei; Freudiger, Christian W; Lu, Sijia; Xie, X Sunney

2011-01-01

The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems. Coherent nonlinear optical imaging, especially the recently developed nonlinear dissipation microscopy (including stimulated Raman scattering and two-photon absorption) and pump-probe microscopy (including excited-state absorption, stimulated emission, and ground-state depletion), provides new image contrasts for nonfluorescent species. Thanks to the high-frequency modulation transfer scheme, these imaging techniques exhibit superb detection sensitivity. By directly interrogating vibrational and/or electronic energy levels of molecules, they offer high molecular specificity. Here we review the underlying principles and excitation and detection schemes, as well as exemplary biomedical applications of this emerging class of molecular imaging techniques.

Microscopy imaging device with advanced imaging properties

DOEpatents

Ghosh, Kunal; Burns, Laurie; El Gamal, Abbas; Schnitzer, Mark J.; Cocker, Eric; Ho, Tatt Wei

2015-11-24

Systems, methods and devices are implemented for microscope imaging solutions. One embodiment of the present disclosure is directed toward an epifluorescence microscope. The microscope includes an image capture circuit including an array of optical sensor. An optical arrangement is configured to direct excitation light of less than about 1 mW to a target object in a field of view of that is at least 0.5 mm.sup.2 and to direct epi-fluorescence emission caused by the excitation light to the array of optical sensors. The optical arrangement and array of optical sensors are each sufficiently close to the target object to provide at least 2.5 .mu.m resolution for an image of the field of view.

Microscopy imaging device with advanced imaging properties

DOEpatents

Ghosh, Kunal; Burns, Laurie; El Gamal, Abbas; Schnitzer, Mark J.; Cocker, Eric; Ho, Tatt Wei

2016-10-25

Systems, methods and devices are implemented for microscope imaging solutions. One embodiment of the present disclosure is directed toward an epifluorescence microscope. The microscope includes an image capture circuit including an array of optical sensor. An optical arrangement is configured to direct excitation light of less than about 1 mW to a target object in a field of view of that is at least 0.5 mm.sup.2 and to direct epi-fluorescence emission caused by the excitation light to the array of optical sensors. The optical arrangement and array of optical sensors are each sufficiently close to the target object to provide at least 2.5 .mu.m resolution for an image of the field of view.

Microscopy imaging device with advanced imaging properties

DOEpatents

Ghosh, Kunal; Burns, Laurie; El Gamal, Abbas; Schnitzer, Mark J.; Cocker, Eric; Ho, Tatt Wei

2016-11-22

Systems, methods and devices are implemented for microscope imaging solutions. One embodiment of the present disclosure is directed toward an epifluorescence microscope. The microscope includes an image capture circuit including an array of optical sensor. An optical arrangement is configured to direct excitation light of less than about 1 mW to a target object in a field of view of that is at least 0.5 mm.sup.2 and to direct epi-fluorescence emission caused by the excitation light to the array of optical sensors. The optical arrangement and array of optical sensors are each sufficiently close to the target object to provide at least 2.5 .mu.m resolution for an image of the field of view.

Microscopy imaging device with advanced imaging properties

DOEpatents

Ghosh, Kunal; Burns, Laurie; El Gamal, Abbas; Schnitzer, Mark J.; Cocker, Eric; Ho, Tatt Wei

2017-04-25

Systems, methods and devices are implemented for microscope imaging solutions. One embodiment of the present disclosure is directed toward an epifluorescence microscope. The microscope includes an image capture circuit including an array of optical sensor. An optical arrangement is configured to direct excitation light of less than about 1 mW to a target object in a field of view of that is at least 0.5 mm.sup.2 and to direct epi-fluorescence emission caused by the excitation light to the array of optical sensors. The optical arrangement and array of optical sensors are each sufficiently close to the target object to provide at least 2.5 .mu.m resolution for an image of the field of view.

110 °C range athermalization of wavefront coding infrared imaging systems

NASA Astrophysics Data System (ADS)

Feng, Bin; Shi, Zelin; Chang, Zheng; Liu, Haizheng; Zhao, Yaohong

2017-09-01

110 °C range athermalization is significant but difficult for designing infrared imaging systems. Our wavefront coding athermalized infrared imaging system adopts an optical phase mask with less manufacturing errors and a decoding method based on shrinkage function. The qualitative experiments prove that our wavefront coding athermalized infrared imaging system has three prominent merits: (1) working well over a temperature range of 110 °C; (2) extending the focal depth up to 15.2 times; (3) achieving a decoded image being approximate to its corresponding in-focus infrared image, with a mean structural similarity index (MSSIM) value greater than 0.85.

Contact CMOS imaging of gaseous oxygen sensor array

PubMed Central

Daivasagaya, Daisy S.; Yao, Lei; Yi Yung, Ka; Hajj-Hassan, Mohamad; Cheung, Maurice C.; Chodavarapu, Vamsy P.; Bright, Frank V.

2014-01-01

We describe a compact luminescent gaseous oxygen (O2) sensor microsystem based on the direct integration of sensor elements with a polymeric optical filter and placed on a low power complementary metal-oxide semiconductor (CMOS) imager integrated circuit (IC). The sensor operates on the measurement of excited-state emission intensity of O2-sensitive luminophore molecules tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) ([Ru(dpp)3]2+) encapsulated within sol–gel derived xerogel thin films. The polymeric optical filter is made with polydimethylsiloxane (PDMS) that is mixed with a dye (Sudan-II). The PDMS membrane surface is molded to incorporate arrays of trapezoidal microstructures that serve to focus the optical sensor signals on to the imager pixels. The molded PDMS membrane is then attached with the PDMS color filter. The xerogel sensor arrays are contact printed on top of the PDMS trapezoidal lens-like microstructures. The CMOS imager uses a 32 × 32 (1024 elements) array of active pixel sensors and each pixel includes a high-gain phototransistor to convert the detected optical signals into electrical currents. Correlated double sampling circuit, pixel address, digital control and signal integration circuits are also implemented on-chip. The CMOS imager data is read out as a serial coded signal. The CMOS imager consumes a static power of 320 µW and an average dynamic power of 625 µW when operating at 100 Hz sampling frequency and 1.8 V DC. This CMOS sensor system provides a useful platform for the development of miniaturized optical chemical gas sensors. PMID:24493909

Contact CMOS imaging of gaseous oxygen sensor array.

PubMed

Daivasagaya, Daisy S; Yao, Lei; Yi Yung, Ka; Hajj-Hassan, Mohamad; Cheung, Maurice C; Chodavarapu, Vamsy P; Bright, Frank V

2011-10-01

We describe a compact luminescent gaseous oxygen (O 2 ) sensor microsystem based on the direct integration of sensor elements with a polymeric optical filter and placed on a low power complementary metal-oxide semiconductor (CMOS) imager integrated circuit (IC). The sensor operates on the measurement of excited-state emission intensity of O 2 -sensitive luminophore molecules tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) ([Ru(dpp) 3 ] 2+ ) encapsulated within sol-gel derived xerogel thin films. The polymeric optical filter is made with polydimethylsiloxane (PDMS) that is mixed with a dye (Sudan-II). The PDMS membrane surface is molded to incorporate arrays of trapezoidal microstructures that serve to focus the optical sensor signals on to the imager pixels. The molded PDMS membrane is then attached with the PDMS color filter. The xerogel sensor arrays are contact printed on top of the PDMS trapezoidal lens-like microstructures. The CMOS imager uses a 32 × 32 (1024 elements) array of active pixel sensors and each pixel includes a high-gain phototransistor to convert the detected optical signals into electrical currents. Correlated double sampling circuit, pixel address, digital control and signal integration circuits are also implemented on-chip. The CMOS imager data is read out as a serial coded signal. The CMOS imager consumes a static power of 320 µW and an average dynamic power of 625 µW when operating at 100 Hz sampling frequency and 1.8 V DC. This CMOS sensor system provides a useful platform for the development of miniaturized optical chemical gas sensors.

Data Compression Techniques for Maps

DTIC Science & Technology

1989-01-01

Lempel - Ziv compression is applied to the classified and unclassified images as also to the output of the compression algorithms . The algorithms ...resulted in a compression of 7:1. The output of the quadtree coding algorithm was then compressed using Lempel - Ziv coding. The compression ratio achieved...using Lempel - Ziv coding. The unclassified image gave a compression ratio of only 1.4:1. The K means classified image

Active infrared thermal imaging technology to detect the corrosion defects in aircraft cargo door

NASA Astrophysics Data System (ADS)

Chen, Dapeng; Zhang, Cunlin; Zeng, Zhi; Xing, Chunfei; Li, Yanhong

2009-11-01

Aircraft fuselage material corrosion problems have been major aviation security issues, which hinder the development of aviation industry. How can we use non-destructive testing methods to detect the internal corrosion defects from the outside of the fuselage, to find the hidden safety problems in advance and update the defective equipment and materials, has great significance for the prevention of accidents. Nowadays, the active infrared thermal imaging technology as a new nondestructive technology has been gradually used on a wide variety of materials, such as composite, metal and so on. This article makes use of this technology on an aircraft cargo door specimen to detect the corrosion defects. Firstly, use High-energy flash pulse to excite the specimen, and use the thermal image processing software to splice the thermal images, so the thermal images of the overall specimen can be showed. Then, heat the defects by ultrasonic excitation, this will cause vibration and friction or thermoelastic effects in the places of defects, so the ultrasonic energy will dissipate into heat and manifested in the uneven temperature of surface. An Infrared camera to capture the changes of temperature of material surface, send data to the computer and records the thermal information of the defects. Finally, extracting data and drawing infrared radiation-time curve of some selected points of interest to analyze the signal changes in heat of defects further more. The results of the experiments show that both of the two ways of heat excitation show a clear position and shape of defects, and the ultrasonic method has more obvious effect of excitation to the defects, and a higher signal to noise ratio than the flash pulse excitation, but flash pulse method do not contact the specimen in the process of excitation, and shows the location and shape of defects in the overall of the specimen has its advantages.

Decomposition of the optical transfer function: wavefront coding imaging systems

NASA Astrophysics Data System (ADS)

Muyo, Gonzalo; Harvey, Andy R.

2005-10-01

We describe the mapping of the optical transfer function (OTF) of an incoherent imaging system into a geometrical representation. We show that for defocused traditional and wavefront-coded systems the OTF can be represented as a generalized Cornu spiral. This representation provides a physical insight into the way in which wavefront coding can increase the depth of field of an imaging system and permits analytical quantification of salient OTF parameters, such as the depth of focus, the location of nulls, and amplitude and phase modulation of the wavefront-coding OTF.

In-Beam Gamma-ray Spectroscopy in the sdpf 37Ar Nucleus

NASA Astrophysics Data System (ADS)

Silveira, M. A. G.; Medina, N. H.; Seale, W. A.; Ribas, R. V.; de Oliveira, J. R. B.; Zilio, S.; Lenzi, S. M.; Napoli, D. R.; Marginean, N.; Vedova, F. Della; Farnea, E.; Ionescu-Bujor, M.; Iordachescu, A.

2007-10-01

The nucleus 37Ar has been studied with γ-ray spectroscopy in the 24Mg(16O,2pn) reaction at a beam energy of 70 MeV. Twenty two new excited states up to an excitation energy of 13 MeV have been observed. We compare the first negative and positive parity yrast states with large-scale-shell-model calculations using the Antoine code and the SDPF interaction, considering the excitation of the 1d5/2,2s1/2 and 1d3/2 nucleons to 1f7/2 and 2p3/2 in the sdpf valence space.

Laser Diagnostics for Reacting Flows

DTIC Science & Technology

2007-01-30

image the exit region of an underexpanded jet. Background IRPLIF involves the excitation of molecular vibrational modes via infrared laser radiation (an...excitation wavelength of 2.0 pm is used for this work), followed by imaging of the subsequent vibrational fluorescence (fluorescence is collected...with an IR-active vibrational mode, such as CO2, are naturally present in sufficient abundance. Even in situations where they need to be added, small

Region-Based Prediction for Image Compression in the Cloud.

PubMed

Begaint, Jean; Thoreau, Dominique; Guillotel, Philippe; Guillemot, Christine

2018-04-01

Thanks to the increasing number of images stored in the cloud, external image similarities can be leveraged to efficiently compress images by exploiting inter-images correlations. In this paper, we propose a novel image prediction scheme for cloud storage. Unlike current state-of-the-art methods, we use a semi-local approach to exploit inter-image correlation. The reference image is first segmented into multiple planar regions determined from matched local features and super-pixels. The geometric and photometric disparities between the matched regions of the reference image and the current image are then compensated. Finally, multiple references are generated from the estimated compensation models and organized in a pseudo-sequence to differentially encode the input image using classical video coding tools. Experimental results demonstrate that the proposed approach yields significant rate-distortion performance improvements compared with the current image inter-coding solutions such as high efficiency video coding.

Image management research

NASA Technical Reports Server (NTRS)

Watson, Andrew B.

1988-01-01

Two types of research issues are involved in image management systems with space station applications: image processing research and image perception research. The image processing issues are the traditional ones of digitizing, coding, compressing, storing, analyzing, and displaying, but with a new emphasis on the constraints imposed by the human perceiver. Two image coding algorithms have been developed that may increase the efficiency of image management systems (IMS). Image perception research involves a study of the theoretical and practical aspects of visual perception of electronically displayed images. Issues include how rapidly a user can search through a library of images, how to make this search more efficient, and how to present images in terms of resolution and split screens. Other issues include optimal interface to an IMS and how to code images in a way that is optimal for the human perceiver. A test-bed within which such issues can be addressed has been designed.

Sub-Selective Quantization for Learning Binary Codes in Large-Scale Image Search.

PubMed

Li, Yeqing; Liu, Wei; Huang, Junzhou

2018-06-01

Recently with the explosive growth of visual content on the Internet, large-scale image search has attracted intensive attention. It has been shown that mapping high-dimensional image descriptors to compact binary codes can lead to considerable efficiency gains in both storage and performing similarity computation of images. However, most existing methods still suffer from expensive training devoted to large-scale binary code learning. To address this issue, we propose a sub-selection based matrix manipulation algorithm, which can significantly reduce the computational cost of code learning. As case studies, we apply the sub-selection algorithm to several popular quantization techniques including cases using linear and nonlinear mappings. Crucially, we can justify the resulting sub-selective quantization by proving its theoretic properties. Extensive experiments are carried out on three image benchmarks with up to one million samples, corroborating the efficacy of the sub-selective quantization method in terms of image retrieval.

Use of fluorescent proteins and color-coded imaging to visualize cancer cells with different genetic properties.

PubMed

Hoffman, Robert M

2016-03-01

Fluorescent proteins are very bright and available in spectrally-distinct colors, enable the imaging of color-coded cancer cells growing in vivo and therefore the distinction of cancer cells with different genetic properties. Non-invasive and intravital imaging of cancer cells with fluorescent proteins allows the visualization of distinct genetic variants of cancer cells down to the cellular level in vivo. Cancer cells with increased or decreased ability to metastasize can be distinguished in vivo. Gene exchange in vivo which enables low metastatic cancer cells to convert to high metastatic can be color-coded imaged in vivo. Cancer stem-like and non-stem cells can be distinguished in vivo by color-coded imaging. These properties also demonstrate the vast superiority of imaging cancer cells in vivo with fluorescent proteins over photon counting of luciferase-labeled cancer cells.

The design of the CMOS wireless bar code scanner applying optical system based on ZigBee

NASA Astrophysics Data System (ADS)

Chen, Yuelin; Peng, Jian

2008-03-01

The traditional bar code scanner is influenced by the length of data line, but the farthest distance of the wireless bar code scanner of wireless communication is generally between 30m and 100m on the market. By rebuilding the traditional CCD optical bar code scanner, a CMOS code scanner is designed based on the ZigBee to meet the demands of market. The scan system consists of the CMOS image sensor and embedded chip S3C2401X, when the two dimensional bar code is read, the results show the inaccurate and wrong code bar, resulted from image defile, disturber, reads image condition badness, signal interference, unstable system voltage. So we put forward the method which uses the matrix evaluation and Read-Solomon arithmetic to solve them. In order to construct the whole wireless optics of bar code system and to ensure its ability of transmitting bar code image signals digitally with long distances, ZigBee is used to transmit data to the base station, and this module is designed based on image acquisition system, and at last the wireless transmitting/receiving CC2430 module circuit linking chart is established. And by transplanting the embedded RTOS system LINUX to the MCU, an applying wireless CMOS optics bar code scanner and multi-task system is constructed. Finally, performance of communication is tested by evaluation software Smart RF. In broad space, every ZIGBEE node can realize 50m transmission with high reliability. When adding more ZigBee nodes, the transmission distance can be several thousands of meters long.

Adaptive image coding based on cubic-spline interpolation

NASA Astrophysics Data System (ADS)

Jiang, Jian-Xing; Hong, Shao-Hua; Lin, Tsung-Ching; Wang, Lin; Truong, Trieu-Kien

2014-09-01

It has been investigated that at low bit rates, downsampling prior to coding and upsampling after decoding can achieve better compression performance than standard coding algorithms, e.g., JPEG and H. 264/AVC. However, at high bit rates, the sampling-based schemes generate more distortion. Additionally, the maximum bit rate for the sampling-based scheme to outperform the standard algorithm is image-dependent. In this paper, a practical adaptive image coding algorithm based on the cubic-spline interpolation (CSI) is proposed. This proposed algorithm adaptively selects the image coding method from CSI-based modified JPEG and standard JPEG under a given target bit rate utilizing the so called ρ-domain analysis. The experimental results indicate that compared with the standard JPEG, the proposed algorithm can show better performance at low bit rates and maintain the same performance at high bit rates.

Comparison of PA imaging by narrow beam scanning and one-shot broad beam excitation

NASA Astrophysics Data System (ADS)

Xia, Jinjun; Wei, Chen-Wei; Huang, Lingyun; Pelivanov, I. M.; O'Donnell, Matthew

2011-03-01

Current systems designed for deep photoacoustic (PA) imaging typically use a low repetition rate, high power pulsed laser to provide a ns-scale pulse illuminating a large tissue volume. Acoustic signals recorded on each laser firing can be used to reconstruct a complete 2-D (3-D) image of sources of heat release within that region. Using broad-beam excitation, the maximum frame rate of the imaging system is restricted by the pulse repetition rate of the laser. An alternate illumination approach is proposed based on fast scanning by a low energy (~ 1 mJ) high repetition rate (up to a few kHz) narrow laser beam (~1 mm) along the tissue surface over a region of interest. A final PA image is produced from the summation of individual PA images reconstructed at each laser beam position. This concept can take advantage of high repetition rate fiber lasers to create PA images with much higher frame rates than current systems, enabling true real-time integration of photoacoustics with ultrasound imaging. As an initial proof of concept, we compare conventional broad beam illumination to a scanned beam approach in a simple model system. Two transparent teflon tubes with diameters of 1.6 mm and 0.8 mm were filled with ink having an absorption coefficient of 5 cm-1. These tubes were buried inside chicken breast tissue acting as an optical scattering medium. They were separated by 3 mm or 10 mm to test spatial and contrast resolution for the two scan formats. The excitation wavelength was 700 nm. The excitation source is a traditional OPO pumped by a Q-switched Nd:YAG laser with doubler. Photoacoustic images were reconstructed using signals from a small, scanned PVDF transducer acting as an acoustic array. Two different illumination schemes were compared: one was 15 mm x 10 mm in cross section and acted as the broad beam; the other was 5 mm x 2 mm in cross section (15 times smaller than the broad beam case) and was scanned over an area equivalent to broad beam illumination. Multiple images obtained during narrow beam scanning were added together to form one PA image equivalent to the single-shot broad beam one. Results of the phantom study indicate that PA images formed by narrow beam scanning excitation can be equivalent to one shot broad beam illumination in signal to noise ratio and spatial resolution. Future studies will focus on high repetition-rate laser sources and scan formats appropriate for real-time, integrated deep photoacoustic/ultrasonic imaging.

Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation

NASA Astrophysics Data System (ADS)

Vuillemin, Nelly; Mahou, Pierre; Débarre, Delphine; Gacoin, Thierry; Tharaux, Pierre-Louis; Schanne-Klein, Marie-Claire; Supatto, Willy; Beaurepaire, Emmanuel

2016-07-01

Second-harmonic generation (SHG) is the most specific label-free indicator of collagen accumulation in widespread pathologies such as fibrosis, and SHG-based measurements hold important potential for biomedical analyses. However, efficient collagen SHG scoring in histological slides is hampered by the limited depth-of-field of usual nonlinear microscopes relying on focused Gaussian beam excitation. In this work we analyze theoretically and experimentally the use of Bessel beam excitation to address this issue. Focused Bessel beams can provide an axially extended excitation volume for nonlinear microscopy while preserving lateral resolution. We show that shaping the focal volume has consequences on signal level and scattering directionality in the case of coherent signals (such as SHG) which significantly differ from the case of incoherent signals (two-photon excited fluorescence, 2PEF). We demonstrate extended-depth SHG-2PEF imaging of fibrotic mouse kidney histological slides. Finally, we show that Bessel beam excitation combined with spatial filtering of the harmonic light in wave vector space can be used to probe collagen accumulation more efficiently than the usual Gaussian excitation scheme. These results open the way to SHG-based histological diagnoses.

Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation

PubMed Central

Vuillemin, Nelly; Mahou, Pierre; Débarre, Delphine; Gacoin, Thierry; Tharaux, Pierre-Louis; Schanne-Klein, Marie-Claire; Supatto, Willy; Beaurepaire, Emmanuel

2016-01-01

Second-harmonic generation (SHG) is the most specific label-free indicator of collagen accumulation in widespread pathologies such as fibrosis, and SHG-based measurements hold important potential for biomedical analyses. However, efficient collagen SHG scoring in histological slides is hampered by the limited depth-of-field of usual nonlinear microscopes relying on focused Gaussian beam excitation. In this work we analyze theoretically and experimentally the use of Bessel beam excitation to address this issue. Focused Bessel beams can provide an axially extended excitation volume for nonlinear microscopy while preserving lateral resolution. We show that shaping the focal volume has consequences on signal level and scattering directionality in the case of coherent signals (such as SHG) which significantly differ from the case of incoherent signals (two-photon excited fluorescence, 2PEF). We demonstrate extended-depth SHG-2PEF imaging of fibrotic mouse kidney histological slides. Finally, we show that Bessel beam excitation combined with spatial filtering of the harmonic light in wave vector space can be used to probe collagen accumulation more efficiently than the usual Gaussian excitation scheme. These results open the way to SHG-based histological diagnoses. PMID:27435390

Thick-target transmission method for excitation functions of interaction cross sections

NASA Astrophysics Data System (ADS)

Aikawa, M.; Ebata, S.; Imai, S.

2016-09-01

We propose a method, called as thick-target transmission (T3) method, to obtain an excitation function of interaction cross sections. In an ordinal experiment to measure the excitation function of interaction cross sections by the transmission method, we need to change the beam energy for each cross section. In the T3 method, the excitation function is derived from the beam attenuations measured at the targets of different thicknesses without changing the beam energy. The advantage of the T3 method is the simplicity and availability for radioactive beams. To confirm the availability, we perform a simulation for the 12C + 27Al system with the PHITS code instead of actual experiments. Our results have large uncertainties but well reproduce the tendency of the experimental data.

Double-Protected All-Inorganic Perovskite Nanocrystals by Crystalline Matrix and Silica for Triple-Modal Anti-Counterfeiting Codes.

PubMed

Xu, Leimeng; Chen, Jiawei; Song, Jizhong; Li, Jianhai; Xue, Jie; Dong, Yuhui; Cai, Bo; Shan, Qingsong; Han, Boning; Zeng, Haibo

2017-08-09

Novel fluorescence with highly covert and reliable features is quite desirable to combat the sophisticated counterfeiters. Herein, we report a simultaneously triple-modal fluorescent characteristic of CsPbBr 3 @Cs 4 PbBr 6 /SiO 2 by the excitation of thermal, ultraviolet (UV) and infrared (IR) light for the first time, which can be applied for the multiple modal anti-counterfeiting codes. The diphasic structure CsPbBr 3 @Cs 4 PbBr 6 nanocrystals (NCs) was synthesized via the typical reprecipitation method followed by uniformly encapsulation into silica microspheres. Cubic CsPbBr 3 is responsible for the functions of anti-counterfeiting, while Cs 4 PbBr 6 crystalline and SiO 2 are mainly to protect unstable CsPbBr 3 NCs from being destroyed by ambient conditions. The as-prepared CsPbBr 3 @Cs 4 PbBr 6 /SiO 2 NCs possess improved stability and are capable of forming printable ink with organic binders for patterns. Interestingly, the fluorescence of diphasic CsPbBr 3 @Cs 4 PbBr 6 /SiO 2 capsule patterns can be reversibly switched by the heating, UV, and IR light irradiation, which has been applied as triple-modal fluorescent anti-counterfeiting codes. The results demonstrate that the perovskite@silica capsules are highly promising for myriad applications in areas such as fluorescent anti-counterfeiting, optoelectronic devices, medical diagnosis, and biological imaging.

2D and 3D Models of Convective Turbulence and Oscillations in Intermediate-Mass Main-Sequence Stars

NASA Astrophysics Data System (ADS)

Guzik, Joyce Ann; Morgan, Taylor H.; Nelson, Nicholas J.; Lovekin, Catherine; Kitiashvili, Irina N.; Mansour, Nagi N.; Kosovichev, Alexander

2015-08-01

We present multidimensional modeling of convection and oscillations in main-sequence stars somewhat more massive than the sun, using three separate approaches: 1) Applying the spherical 3D MHD ASH (Anelastic Spherical Harmonics) code to simulate the core convection and radiative zone. Our goal is to determine whether core convection can excite low-frequency gravity modes, and thereby explain the presence of low frequencies for some hybrid gamma Dor/delta Sct variables for which the envelope convection zone is too shallow for the convective blocking mechanism to drive g modes; 2) Using the 3D planar ‘StellarBox’ radiation hydrodynamics code to model the envelope convection zone and part of the radiative zone. Our goals are to examine the interaction of stellar pulsations with turbulent convection in the envelope, excitation of acoustic modes, and the role of convective overshooting; 3) Applying the ROTORC 2D stellar evolution and dynamics code to calculate evolution with a variety of initial rotation rates and extents of core convective overshooting. The nonradial adiabatic pulsation frequencies of these nonspherical models will be calculated using the 2D pulsation code NRO of Clement. We will present new insights into gamma Dor and delta Sct pulsations gained by multidimensional modeling compared to 1D model expectations.

Correlating two-photon excited fluorescence imaging of breast cancer cellular redox state with seahorse flux analysis of normalized cellular oxygen consumption

NASA Astrophysics Data System (ADS)

Hou, Jue; Wright, Heather J.; Chan, Nicole; Tran, Richard; Razorenova, Olga V.; Potma, Eric O.; Tromberg, Bruce J.

2016-06-01

Two-photon excited fluorescence (TPEF) imaging of the cellular cofactors nicotinamide adenine dinucleotide and oxidized flavin adenine dinucleotide is widely used to measure cellular metabolism, both in normal and pathological cells and tissues. When dual-wavelength excitation is used, ratiometric TPEF imaging of the intrinsic cofactor fluorescence provides a metabolic index of cells-the "optical redox ratio" (ORR). With increased interest in understanding and controlling cellular metabolism in cancer, there is a need to evaluate the performance of ORR in malignant cells. We compare TPEF metabolic imaging with seahorse flux analysis of cellular oxygen consumption in two different breast cancer cell lines (MCF-7 and MDA-MB-231). We monitor metabolic index in living cells under both normal culture conditions and, for MCF-7, in response to cell respiration inhibitors and uncouplers. We observe a significant correlation between the TPEF-derived ORR and the flux analyzer measurements (R=0.7901, p<0.001). Our results confirm that the ORR is a valid dynamic index of cell metabolism under a range of oxygen consumption conditions relevant for cancer imaging.

Autofluorescence-based diagnostic UV imaging of tissues and cells

NASA Astrophysics Data System (ADS)

Renkoski, Timothy E.

Cancer is the second leading cause of death in the United States, and its early diagnosis is critical to improving treatment options and patient outcomes. In autofluorescence (AF) imaging, light of controlled wavelengths is projected onto tissue, absorbed by specific molecules, and re-emitted at longer wavelengths. Images of re-emitted light are used together with spectral information to infer tissue functional information and diagnosis. This dissertation describes AF imaging studies of three different organs using data collected from fresh human surgical specimens. In the ovary study, illumination was at 365 nm, and images were captured at 8 emission wavelengths. Measurements from a multispectral imaging system and fiber optic probe were used to map tissue diagnosis at every image pixel. For the colon and pancreas studies, instrumentation was developed extending AF imaging capability to sub-300 nm excitation. Images excited in the deep UV revealed tryptophan and protein content which are believed to change with disease state. Several excitation wavelength bands from 280 nm to 440 nm were investigated. Microscopic AF images collected in the pancreas study included both cultured and primary cells. Several findings are reported. A method of transforming fiber optic probe spectra for direct comparison with imager spectra was devised. Normalization of AF data by green reflectance data was found useful in correcting hemoglobin absorption. Ratio images, both AF and reflectance, were formulated to highlight growths in the colon. Novel tryptophan AF images were found less useful for colon diagnostics than the new ratio techniques. Microscopic tryptophan AF images produce useful visualization of cellular protein content, but their diagnostic value requires further study.

Modeling and boundary force control of microcantilevers utilized in atomic force microscopy for cellular imaging and characterization

NASA Astrophysics Data System (ADS)

Eslami, Sohrab

This dissertation undertakes the theoretical and experimental developments microcantilevers utilized in Atomic Force Microscopy (AFM) with applications to cellular imaging and characterization. The capability of revealing the inhomogeneties or interior of ultra-small materials has been of most interest to many researchers. However, the fundamental concept of signal and image formation remains unexplored and not fully understood. For his, a semi-empirical nonlinear force model is proposed to show that virtual frequency generation, regarded as the simplest synthesized subsurface probe, occurs optimally when the force is tuned to the van der Waals form. This is the first-time observation of a novel theoretical dynamic multi-frequency force microscopy that has not been already reported. Owing to the broad applications of microcantilevers in the nanoscale imaging and microscopic techniques, there is an essential feeling to study and propose a comprehensive model of such systems. Therefore, in the theoretical part of this dissertation, a distributed-parameters representation modeling of the microcantilever along with a general interaction force comprising of two attractive and repulsive components with general amplitude and power terms is studied. This model is investigated in a general 2D Cartesian coordinate to consider the motions of the probe with a tip mass. There is an excitation at the microcantilever's base such that the end of the beam is subject to the proposed general force. These forces are very sensitive to the amplitude and power terms of these parts; on the other hand, atomic intermolecular force is a function of the distance such that this distance itself is also a function of the interaction force that will result in a nonlinear implicit equation. From a parametric study in the probe-sample excitation, it is shown that the predicted behavior of the generated difference-frequency oscillation amplitude agrees well with experimental measurements. Following the proposed Euler-Bernoulli model, a more comprehensive model is developed by modeling the probe dynamics and including the effects of the rotary inertia and shear deformation under the same proposed tip-sample interaction force. An extensive comparative study between the Euler-Bernoulli and Timoshenko beam assumptions is conducted for different conditions including different base-excitation amplitudes and higher modes. The results underline that the comprehensive Timoshenko model unveils the effects of the nonlinear interaction force better than the Euler-Bernoulli beam model. In addition to extensive modeling efforts on the microcantilever and its interaction with sample, an adaptive control framework is developed in order to make the microcantilever's tip follow a desired trajectory. This trajectory can further be considered as an important path acquired by the path planning techniques to manipulate the nanoparticles. There is a base excitation considered for this model and can be considered as an input force control to excite the probe by taking advantage of flexibility of the cantilever despite its complexity and under existence of the external nonlinear interaction forces between the tip and sample's surface. When building such complicated controller on top of the proposed comprehensive model, the results could be extended to study a macro-micro hybrid rigid-flexible model of a microrobot to mimic the realistic behavior of the MM3ARTM microrobot. The MM3ARTM microrobot is equipped with a piezoresistive layer which functions as a force sensor and is capable of measuring very slight forces as small as micro to nano-Newton. Two types of controllers are investigated for the case of the tip force control. Lyapunov-based PD and robust adaptive controllers are developed for this purpose and their performances and stabilities are compared. In the experimental part, a platform for performing the automated nanomanipulation and real-time cellular imaging is developed by integrating a microrobot, digital signal processor platform (dSPACERTM), computer, and a state-of-the-art light microscope. The closed-loop boundary force control framework is additionally developed for the autonomous in-situ applications. Since the incoming and outgoing signals of the piezoresistive microrobot are in the form of the electrical voltage and the string commands (ASCII code), respectively, an intuitive programming code for interfacing the MATLAB and dSPACE RTM has been written for the online quasi-data acquisition. As a result, the height of the corneal cell has been obtained and additionally, the microcantilever's tip force has been automatically controlled by taking advantage of the proposed control framework.

Block-based scalable wavelet image codec

NASA Astrophysics Data System (ADS)

Bao, Yiliang; Kuo, C.-C. Jay

1999-10-01

This paper presents a high performance block-based wavelet image coder which is designed to be of very low implementational complexity yet with rich features. In this image coder, the Dual-Sliding Wavelet Transform (DSWT) is first applied to image data to generate wavelet coefficients in fixed-size blocks. Here, a block only consists of wavelet coefficients from a single subband. The coefficient blocks are directly coded with the Low Complexity Binary Description (LCBiD) coefficient coding algorithm. Each block is encoded using binary context-based bitplane coding. No parent-child correlation is exploited in the coding process. There is also no intermediate buffering needed in between DSWT and LCBiD. The compressed bit stream generated by the proposed coder is both SNR and resolution scalable, as well as highly resilient to transmission errors. Both DSWT and LCBiD process the data in blocks whose size is independent of the size of the original image. This gives more flexibility in the implementation. The codec has a very good coding performance even the block size is (16,16).

Adaptive partially hidden Markov models with application to bilevel image coding.

PubMed

Forchhammer, S; Rasmussen, T S

1999-01-01

Partially hidden Markov models (PHMMs) have previously been introduced. The transition and emission/output probabilities from hidden states, as known from the HMMs, are conditioned on the past. This way, the HMM may be applied to images introducing the dependencies of the second dimension by conditioning. In this paper, the PHMM is extended to multiple sequences with a multiple token version and adaptive versions of PHMM coding are presented. The different versions of the PHMM are applied to lossless bilevel image coding. To reduce and optimize the model cost and size, the contexts are organized in trees and effective quantization of the parameters is introduced. The new coding methods achieve results that are better than the JBIG standard on selected test images, although at the cost of increased complexity. By the minimum description length principle, the methods presented for optimizing the code length may apply as guidance for training (P)HMMs for, e.g., segmentation or recognition purposes. Thereby, the PHMM models provide a new approach to image modeling.

An efficient system for reliably transmitting image and video data over low bit rate noisy channels

NASA Technical Reports Server (NTRS)

Costello, Daniel J., Jr.; Huang, Y. F.; Stevenson, Robert L.

1994-01-01

This research project is intended to develop an efficient system for reliably transmitting image and video data over low bit rate noisy channels. The basic ideas behind the proposed approach are the following: employ statistical-based image modeling to facilitate pre- and post-processing and error detection, use spare redundancy that the source compression did not remove to add robustness, and implement coded modulation to improve bandwidth efficiency and noise rejection. Over the last six months, progress has been made on various aspects of the project. Through our studies of the integrated system, a list-based iterative Trellis decoder has been developed. The decoder accepts feedback from a post-processor which can detect channel errors in the reconstructed image. The error detection is based on the Huber Markov random field image model for the compressed image. The compression scheme used here is that of JPEG (Joint Photographic Experts Group). Experiments were performed and the results are quite encouraging. The principal ideas here are extendable to other compression techniques. In addition, research was also performed on unequal error protection channel coding, subband vector quantization as a means of source coding, and post processing for reducing coding artifacts. Our studies on unequal error protection (UEP) coding for image transmission focused on examining the properties of the UEP capabilities of convolutional codes. The investigation of subband vector quantization employed a wavelet transform with special emphasis on exploiting interband redundancy. The outcome of this investigation included the development of three algorithms for subband vector quantization. The reduction of transform coding artifacts was studied with the aid of a non-Gaussian Markov random field model. This results in improved image decompression. These studies are summarized and the technical papers included in the appendices.

4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM)

PubMed Central

Lavagnino, Zeno; Sancataldo, Giuseppe; d’Amora, Marta; Follert, Philipp; De Pietri Tonelli, Davide; Diaspro, Alberto; Cella Zanacchi, Francesca

2016-01-01

In the last decade light sheet fluorescence microscopy techniques, such as selective plane illumination microscopy (SPIM), has become a well established method for developmental biology. However, conventional SPIM architectures hardly permit imaging of certain tissues since the common sample mounting procedure, based on gel embedding, could interfere with the sample morphology. In this work we propose an inverted selective plane microscopy system (iSPIM), based on non-linear excitation, suitable for 3D tissue imaging. First, the iSPIM architecture provides flexibility on the sample mounting, getting rid of the gel-based mounting typical of conventional SPIM, permitting 3D imaging of hippocampal slices from mouse brain. Moreover, all the advantages brought by two photon excitation (2PE) in terms of reduction of scattering effects and contrast improvement are exploited, demonstrating an improved image quality and contrast compared to single photon excitation. The system proposed represents an optimal platform for tissue imaging and it smooths the way to the applicability of light sheet microscopy to a wider range of samples including those that have to be mounted on non-transparent surfaces. PMID:27033347

Code-modulated interferometric imaging system using phased arrays

NASA Astrophysics Data System (ADS)

Chauhan, Vikas; Greene, Kevin; Floyd, Brian

2016-05-01

Millimeter-wave (mm-wave) imaging provides compelling capabilities for security screening, navigation, and bio- medical applications. Traditional scanned or focal-plane mm-wave imagers are bulky and costly. In contrast, phased-array hardware developed for mass-market wireless communications and automotive radar promise to be extremely low cost. In this work, we present techniques which can allow low-cost phased-array receivers to be reconfigured or re-purposed as interferometric imagers, removing the need for custom hardware and thereby reducing cost. Since traditional phased arrays power combine incoming signals prior to digitization, orthogonal code-modulation is applied to each incoming signal using phase shifters within each front-end and two-bit codes. These code-modulated signals can then be combined and processed coherently through a shared hardware path. Once digitized, visibility functions can be recovered through squaring and code-demultiplexing operations. Pro- vided that codes are selected such that the product of two orthogonal codes is a third unique and orthogonal code, it is possible to demultiplex complex visibility functions directly. As such, the proposed system modulates incoming signals but demodulates desired correlations. In this work, we present the operation of the system, a validation of its operation using behavioral models of a traditional phased array, and a benchmarking of the code-modulated interferometer against traditional interferometer and focal-plane arrays.

DFTBaby: A software package for non-adiabatic molecular dynamics simulations based on long-range corrected tight-binding TD-DFT(B)

NASA Astrophysics Data System (ADS)

Humeniuk, Alexander; Mitrić, Roland

2017-12-01

A software package, called DFTBaby, is published, which provides the electronic structure needed for running non-adiabatic molecular dynamics simulations at the level of tight-binding DFT. A long-range correction is incorporated to avoid spurious charge transfer states. Excited state energies, their analytic gradients and scalar non-adiabatic couplings are computed using tight-binding TD-DFT. These quantities are fed into a molecular dynamics code, which integrates Newton's equations of motion for the nuclei together with the electronic Schrödinger equation. Non-adiabatic effects are included by surface hopping. As an example, the program is applied to the optimization of excited states and non-adiabatic dynamics of polyfluorene. The python and Fortran source code is available at http://www.dftbaby.chemie.uni-wuerzburg.de.

Optical information authentication using compressed double-random-phase-encoded images and quick-response codes.

PubMed

Wang, Xiaogang; Chen, Wen; Chen, Xudong

2015-03-09

In this paper, we develop a new optical information authentication system based on compressed double-random-phase-encoded images and quick-response (QR) codes, where the parameters of optical lightwave are used as keys for optical decryption and the QR code is a key for verification. An input image attached with QR code is first optically encoded in a simplified double random phase encoding (DRPE) scheme without using interferometric setup. From the single encoded intensity pattern recorded by a CCD camera, a compressed double-random-phase-encoded image, i.e., the sparse phase distribution used for optical decryption, is generated by using an iterative phase retrieval technique with QR code. We compare this technique to the other two methods proposed in literature, i.e., Fresnel domain information authentication based on the classical DRPE with holographic technique and information authentication based on DRPE and phase retrieval algorithm. Simulation results show that QR codes are effective on improving the security and data sparsity of optical information encryption and authentication system.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hellfeld, Daniel; Barton, Paul; Gunter, Donald

Gamma-ray imaging facilitates the efficient detection, characterization, and localization of compact radioactive sources in cluttered environments. Fieldable detector systems employing active planar coded apertures have demonstrated broad energy sensitivity via both coded aperture and Compton imaging modalities. But, planar configurations suffer from a limited field-of-view, especially in the coded aperture mode. In order to improve upon this limitation, we introduce a novel design by rearranging the detectors into an active coded spherical configuration, resulting in a 4pi isotropic field-of-view for both coded aperture and Compton imaging. This work focuses on the low- energy coded aperture modality and the optimization techniquesmore » used to determine the optimal number and configuration of 1 cm 3 CdZnTe coplanar grid detectors on a 14 cm diameter sphere with 192 available detector locations.« less

Optical encryption of digital data in form of quick response code using spatially incoherent illumination

NASA Astrophysics Data System (ADS)

Cheremkhin, Pavel A.; Krasnov, Vitaly V.; Rodin, Vladislav G.; Starikov, Rostislav S.

2016-11-01

Applications of optical methods for encryption purposes have been attracting interest of researchers for decades. The most popular are coherent techniques such as double random phase encoding. Its main advantage is high security due to transformation of spectrum of image to be encrypted into white spectrum via use of first phase random mask which allows for encrypted images with white spectra. Downsides are necessity of using holographic registration scheme and speckle noise occurring due to coherent illumination. Elimination of these disadvantages is possible via usage of incoherent illumination. In this case, phase registration no longer matters, which means that there is no need for holographic setup, and speckle noise is gone. Recently, encryption of digital information in form of binary images has become quite popular. Advantages of using quick response (QR) code in capacity of data container for optical encryption include: 1) any data represented as QR code will have close to white (excluding zero spatial frequency) Fourier spectrum which have good overlapping with encryption key spectrum; 2) built-in algorithm for image scale and orientation correction which simplifies decoding of decrypted QR codes; 3) embedded error correction code allows for successful decryption of information even in case of partial corruption of decrypted image. Optical encryption of digital data in form QR codes using spatially incoherent illumination was experimentally implemented. Two liquid crystal spatial light modulators were used in experimental setup for QR code and encrypting kinoform imaging respectively. Decryption was conducted digitally. Successful decryption of encrypted QR codes is demonstrated.

Visual information processing; Proceedings of the Meeting, Orlando, FL, Apr. 20-22, 1992

NASA Technical Reports Server (NTRS)

Huck, Friedrich O. (Editor); Juday, Richard D. (Editor)

1992-01-01

Topics discussed in these proceedings include nonlinear processing and communications; feature extraction and recognition; image gathering, interpolation, and restoration; image coding; and wavelet transform. Papers are presented on noise reduction for signals from nonlinear systems; driving nonlinear systems with chaotic signals; edge detection and image segmentation of space scenes using fractal analyses; a vision system for telerobotic operation; a fidelity analysis of image gathering, interpolation, and restoration; restoration of images degraded by motion; and information, entropy, and fidelity in visual communication. Attention is also given to image coding methods and their assessment, hybrid JPEG/recursive block coding of images, modified wavelets that accommodate causality, modified wavelet transform for unbiased frequency representation, and continuous wavelet transform of one-dimensional signals by Fourier filtering.

A Comparative Study on Diagnostic Accuracy of Colour Coded Digital Images, Direct Digital Images and Conventional Radiographs for Periapical Lesions – An In Vitro Study

PubMed Central

Mubeen; K.R., Vijayalakshmi; Bhuyan, Sanat Kumar; Panigrahi, Rajat G; Priyadarshini, Smita R; Misra, Satyaranjan; Singh, Chandravir

2014-01-01

Objectives: The identification and radiographic interpretation of periapical bone lesions is important for accurate diagnosis and treatment. The present study was undertaken to study the feasibility and diagnostic accuracy of colour coded digital radiographs in terms of presence and size of lesion and to compare the diagnostic accuracy of colour coded digital images with direct digital images and conventional radiographs for assessing periapical lesions. Materials and Methods: Sixty human dry cadaver hemimandibles were obtained and periapical lesions were created in first and second premolar teeth at the junction of cancellous and cortical bone using a micromotor handpiece and carbide burs of sizes 2, 4 and 6. After each successive use of round burs, a conventional, RVG and colour coded image was taken for each specimen. All the images were evaluated by three observers. The diagnostic accuracy for each bur and image mode was calculated statistically. Results: Our results showed good interobserver (kappa > 0.61) agreement for the different radiographic techniques and for the different bur sizes. Conventional Radiography outperformed Digital Radiography in diagnosing periapical lesions made with Size two bur. Both were equally diagnostic for lesions made with larger bur sizes. Colour coding method was least accurate among all the techniques. Conclusion: Conventional radiography traditionally forms the backbone in the diagnosis, treatment planning and follow-up of periapical lesions. Direct digital imaging is an efficient technique, in diagnostic sense. Colour coding of digital radiography was feasible but less accurate however, this imaging technique, like any other, needs to be studied continuously with the emphasis on safety of patients and diagnostic quality of images. PMID:25584318

Synchrotron x-ray imaging of acoustic cavitation bubbles induced by acoustic excitation

NASA Astrophysics Data System (ADS)

Jung, Sung Yong; Park, Han Wook; Park, Sung Ho; Lee, Sang Joon

2017-04-01

The cavitation induced by acoustic excitation has been widely applied in various biomedical applications because cavitation bubbles can enhance the exchanges of mass and energy. In order to minimize the hazardous effects of the induced cavitation, it is essential to understand the spatial distribution of cavitation bubbles. The spatial distribution of cavitation bubbles visualized by the synchrotron x-ray imaging technique is compared to that obtained with a conventional x-ray tube. Cavitation bubbles with high density in the region close to the tip of the probe are visualized using the synchrotron x-ray imaging technique, however, the spatial distribution of cavitation bubbles in the whole ultrasound field is not detected. In this study, the effects of the ultrasound power of acoustic excitation and working medium on the shape and density of the induced cavitation bubbles are examined. As a result, the synchrotron x-ray imaging technique is useful for visualizing spatial distributions of cavitation bubbles, and it could be used for optimizing the operation conditions of acoustic cavitation.

Fluorescence endoscopy using fiber speckle illumination

NASA Astrophysics Data System (ADS)

Nakano, Shuhei; Katagiri, Takashi; Matsuura, Yuji

2018-02-01

An endoscopic fluorescence imaging system based on fiber speckle illumination is proposed. In this system, a multimode fiber for transmission of excitation laser light and collection of fluorescence is inserted into a conventional flexible endoscope. Since the excitation laser light has random speckle structure, one can detect fluorescence signal corresponding to the irradiation pattern if the sample contains fluorophores. The irradiation pattern can be captured by the endoscope camera when the excitation wavelength is within the sensitivity range of the camera. By performing multiple measurements while changing the irradiation pattern, a fluorescence image is reconstructed by solving a norm minimization problem. The principle of our method was experimentally demonstrated. A 2048 pixels image of quantum dots coated on a frosted glass was successfully reconstructed by 32 measurements. We also confirmed that our method can be applied on biological tissues.

Operational rate-distortion performance for joint source and channel coding of images.

PubMed

Ruf, M J; Modestino, J W

1999-01-01

This paper describes a methodology for evaluating the operational rate-distortion behavior of combined source and channel coding schemes with particular application to images. In particular, we demonstrate use of the operational rate-distortion function to obtain the optimum tradeoff between source coding accuracy and channel error protection under the constraint of a fixed transmission bandwidth for the investigated transmission schemes. Furthermore, we develop information-theoretic bounds on performance for specific source and channel coding systems and demonstrate that our combined source-channel coding methodology applied to different schemes results in operational rate-distortion performance which closely approach these theoretical limits. We concentrate specifically on a wavelet-based subband source coding scheme and the use of binary rate-compatible punctured convolutional (RCPC) codes for transmission over the additive white Gaussian noise (AWGN) channel. Explicit results for real-world images demonstrate the efficacy of this approach.

Spectral fitting, shock layer modeling, and production of nitrogen oxides and excited nitrogen

NASA Technical Reports Server (NTRS)

Blackwell, H. E.

1991-01-01

An analysis was made of N2 emission from 8.72 MJ/kg shock layer at 2.54, 1.91, and 1.27 cm positions and vibrational state distributions, temperatures, and relative electronic state populations was obtained from data sets. Other recorded arc jet N2 and air spectral data were reviewed and NO emission characteristics were studied. A review of operational procedures of the DSMC code was made. Information on other appropriate codes and modifications, including ionization, were made as well as a determination of the applicability of codes reviewed to task requirement. A review was also made of computational procedures used in CFD codes of Li and other codes on JSC computers. An analysis was made of problems associated with integration of specific chemical kinetics applicable to task into CFD codes.

Compressive Sampling based Image Coding for Resource-deficient Visual Communication.

PubMed

Liu, Xianming; Zhai, Deming; Zhou, Jiantao; Zhang, Xinfeng; Zhao, Debin; Gao, Wen

2016-04-14

In this paper, a new compressive sampling based image coding scheme is developed to achieve competitive coding efficiency at lower encoder computational complexity, while supporting error resilience. This technique is particularly suitable for visual communication with resource-deficient devices. At the encoder, compact image representation is produced, which is a polyphase down-sampled version of the input image; but the conventional low-pass filter prior to down-sampling is replaced by a local random binary convolution kernel. The pixels of the resulting down-sampled pre-filtered image are local random measurements and placed in the original spatial configuration. The advantages of local random measurements are two folds: 1) preserve high-frequency image features that are otherwise discarded by low-pass filtering; 2) remain a conventional image and can therefore be coded by any standardized codec to remove statistical redundancy of larger scales. Moreover, measurements generated by different kernels can be considered as multiple descriptions of the original image and therefore the proposed scheme has the advantage of multiple description coding. At the decoder, a unified sparsity-based soft-decoding technique is developed to recover the original image from received measurements in a framework of compressive sensing. Experimental results demonstrate that the proposed scheme is competitive compared with existing methods, with a unique strength of recovering fine details and sharp edges at low bit-rates.

A denoising algorithm for CT image using low-rank sparse coding

NASA Astrophysics Data System (ADS)

Lei, Yang; Xu, Dong; Zhou, Zhengyang; Wang, Tonghe; Dong, Xue; Liu, Tian; Dhabaan, Anees; Curran, Walter J.; Yang, Xiaofeng

2018-03-01

We propose a denoising method of CT image based on low-rank sparse coding. The proposed method constructs an adaptive dictionary of image patches and estimates the sparse coding regularization parameters using the Bayesian interpretation. A low-rank approximation approach is used to simultaneously construct the dictionary and achieve sparse representation through clustering similar image patches. A variable-splitting scheme and a quadratic optimization are used to reconstruct CT image based on achieved sparse coefficients. We tested this denoising technology using phantom, brain and abdominal CT images. The experimental results showed that the proposed method delivers state-of-art denoising performance, both in terms of objective criteria and visual quality.

Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy

PubMed Central

Hunter, Jennifer J.; Masella, Benjamin; Dubra, Alfredo; Sharma, Robin; Yin, Lu; Merigan, William H.; Palczewska, Grazyna; Palczewski, Krzysztof; Williams, David R.

2011-01-01

In vivo two-photon imaging through the pupil of the primate eye has the potential to become a useful tool for functional imaging of the retina. Two-photon excited fluorescence images of the macaque cone mosaic were obtained using a fluorescence adaptive optics scanning laser ophthalmoscope, overcoming the challenges of a low numerical aperture, imperfect optics of the eye, high required light levels, and eye motion. Although the specific fluorophores are as yet unknown, strong in vivo intrinsic fluorescence allowed images of the cone mosaic. Imaging intact ex vivo retina revealed that the strongest two-photon excited fluorescence signal comes from the cone inner segments. The fluorescence response increased following light stimulation, which could provide a functional measure of the effects of light on photoreceptors. PMID:21326644

Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM).

PubMed

Yamamoto, Shin; Oshima, Yusuke; Saitou, Takashi; Watanabe, Takao; Miyake, Teruki; Yoshida, Osamu; Tokumoto, Yoshio; Abe, Masanori; Matsuura, Bunzo; Hiasa, Yoichi; Imamura, Takeshi

2016-12-01

Non-alcoholic steatohepatitis (NASH) is a common liver disorder caused by fatty liver. Because NASH is associated with fibrotic and morphological changes in liver tissue, a direct imaging technique is required for accurate staging of liver tissue. For this purpose, in this study we took advantage of two label-free optical imaging techniques, second harmonic generation (SHG) and auto-fluorescence (AF), using two-photon excitation microscopy (TPEM). Three-dimensional ex vivo imaging of tissues from NASH model mice, followed by image processing, revealed that SHG and AF are sufficient to quantitatively characterize the hepatic capsule at an early stage and parenchymal morphologies associated with liver disease progression, respectively.

Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology.

PubMed

Wagener, Kerstin C; Kolbrink, Benedikt; Dietrich, Katharina; Kizina, Kathrin M; Terwitte, Lukas S; Kempkes, Belinda; Bao, Guobin; Müller, Michael

2016-07-01

Reactive oxygen species (ROS) and downstream redox alterations not only mediate physiological signaling but also neuropathology. For long, ROS/redox imaging was hampered by a lack of reliable probes. Genetically encoded redox sensors overcame this gap and revolutionized (sub)cellular redox imaging. Yet, the successful delivery of sensor-coding DNA, which demands transfection/transduction of cultured preparations or stereotaxic microinjections of each subject, remains challenging. By generating transgenic mice, we aimed to overcome limiting cultured preparations, circumvent surgical interventions, and to extend effectively redox imaging to complex and adult preparations. Our redox indicator mice widely express Thy1-driven roGFP1 (reduction-oxidation-sensitive green fluorescent protein 1) in neuronal cytosol or mitochondria. Negative phenotypic effects of roGFP1 were excluded and its proper targeting and functionality confirmed. Redox mapping by ratiometric wide-field imaging reveals most oxidizing conditions in CA3 neurons. Furthermore, mitochondria are more oxidized than cytosol. Cytosolic and mitochondrial roGFP1s reliably report cell endogenous redox dynamics upon metabolic challenge or stimulation. Fluorescence lifetime imaging yields stable, but marginal, response ranges. We therefore developed automated excitation ratiometric 2-photon imaging. It offers superior sensitivity, spatial resolution, and response dynamics. Redox indicator mice enable quantitative analyses of subcellular redox dynamics in a multitude of preparations and at all postnatal stages. This will uncover cell- and compartment-specific cerebral redox signals and their defined alterations during development, maturation, and aging. Cross-breeding with other disease models will reveal molecular details on compartmental redox homeostasis in neuropathology. Combined with ratiometric 2-photon imaging, this will foster our mechanistic understanding of cellular redox signals in their full complexity. Antioxid. Redox Signal. 25, 41-58.

Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology

PubMed Central

Wagener, Kerstin C.; Kolbrink, Benedikt; Dietrich, Katharina; Kizina, Kathrin M.; Terwitte, Lukas S.; Kempkes, Belinda; Bao, Guobin

2016-01-01

Abstract Aims: Reactive oxygen species (ROS) and downstream redox alterations not only mediate physiological signaling but also neuropathology. For long, ROS/redox imaging was hampered by a lack of reliable probes. Genetically encoded redox sensors overcame this gap and revolutionized (sub)cellular redox imaging. Yet, the successful delivery of sensor-coding DNA, which demands transfection/transduction of cultured preparations or stereotaxic microinjections of each subject, remains challenging. By generating transgenic mice, we aimed to overcome limiting cultured preparations, circumvent surgical interventions, and to extend effectively redox imaging to complex and adult preparations. Results: Our redox indicator mice widely express Thy1-driven roGFP1 (reduction–oxidation-sensitive green fluorescent protein 1) in neuronal cytosol or mitochondria. Negative phenotypic effects of roGFP1 were excluded and its proper targeting and functionality confirmed. Redox mapping by ratiometric wide-field imaging reveals most oxidizing conditions in CA3 neurons. Furthermore, mitochondria are more oxidized than cytosol. Cytosolic and mitochondrial roGFP1s reliably report cell endogenous redox dynamics upon metabolic challenge or stimulation. Fluorescence lifetime imaging yields stable, but marginal, response ranges. We therefore developed automated excitation ratiometric 2-photon imaging. It offers superior sensitivity, spatial resolution, and response dynamics. Innovation and Conclusion: Redox indicator mice enable quantitative analyses of subcellular redox dynamics in a multitude of preparations and at all postnatal stages. This will uncover cell- and compartment-specific cerebral redox signals and their defined alterations during development, maturation, and aging. Cross-breeding with other disease models will reveal molecular details on compartmental redox homeostasis in neuropathology. Combined with ratiometric 2-photon imaging, this will foster our mechanistic understanding of cellular redox signals in their full complexity. Antioxid. Redox Signal. 25, 41–58. PMID:27059697

A constructive nonlinear array (CNA) method for barely visible impact detection in composite materials

NASA Astrophysics Data System (ADS)

Malfense Fierro, Gian Piero; Meo, Michele

2017-04-01

Currently there are numerous phased array techniques such as Full Matrix Capture (FMC) and Total Focusing Method (TFM) that provide good damage assessment for composite materials. Although, linear methods struggle to evaluate and assess low levels of damage, while nonlinear methods have shown great promise in early damage detection. A sweep and subtraction evaluation method coupled with a constructive nonlinear array method (CNA) is proposed in order to assess damage specific nonlinearities, address issues with frequency selection when using nonlinear ultrasound imaging techniques and reduce equipment generated nonlinearities. These methods were evaluated using multiple excitation locations on an impacted composite panel with a complex damage (barely visible impact damage). According to various recent works, damage excitation can be accentuated by exciting at local defect resonance (LDR) frequencies; although these frequencies are not always easily determinable. The sweep methodology uses broadband excitation to determine both local defect and material resonances, by assessing local defect generated nonlinearities using a laser vibrometer it is possible to assess which frequencies excite the complex geometry of the crack. The dual effect of accurately determining local defect resonances, the use of an image subtraction method and the reduction of equipment based nonlinearities using CNA result in greater repeatability and clearer nonlinear imaging (NIM).

Toward Fourier interferometry fluorescence excitation/emission imaging of malignant cells combined with photoacoustic microscopy

NASA Astrophysics Data System (ADS)

Kohen, Elli; Hirschberg, Joseph G.; Berry, John P.; Ozkutuk, Nuri; Ornek, Ceren; Monti, Marco; Leblanc, Roger M.; Schachtschabel, Dietrich O.; Haroon, Sumaira

2003-10-01

Dual excitation fluorescence imaging has been used as a first step towards multi-wavelength excitation/emission fluorescence spectral imaging. Target cells are transformed keratinocytes, and other osteosarcoma, human breast and color cancer cells. Mitochondrial membrane potential probes, e.g. TMRM (tetramethylrhodamine methyl ester), Mitotracker Green (Molecular Probes, Inc., Eugene OR,USA; a recently synthesized mitochondrial oxygen probe, [PRE,P1"- pyrene butyl)-2-rhodamine ester] allow dual excitation in the UV plus in teh blue-green spectral regions. Also, using the natural endogenous probe NAD(P)H, preliminary results indicate mitochondrial responses to metabolic challenges (e.g. glucose addition), plus changes in mitochonrial distribution and morphology. In terms of application to biomedicine (for diagnostiscs, prognostsics and drug trials) three parameters have been selected in addition to the natural probe NAD(P)H, i.e. vital fluorescence probing of mitochondria, lysosomes and Golgi apparatus. It is hoped that such a multiparameter approach will allow malignant cell characterization and grading. A new area being introduced is the use of similar methodology for biotechnical applications such as the study of the hydrogen-producing alga Chlamydomonas Reinhardtii, and possible agricultural applications, such as Saccharomyces yeast for oenology. Complementation by Photoacoustic Microscopy is also contemplated, to study the internal conversion component which follows the excitation by photons.

U 3Si 2 Fabrication and Testing for Implementation into the BISON Fuel Performance Code

DOE Office of Scientific and Technical Information (OSTI.GOV)

Knight, Travis W.

A creep test stand was designed and constructed for compressive creep testing of U 3Si 2 pellets. This is described in Chapter 3. Creep testing of U 3Si 2 pellets was completed. In total, 13 compressive creep tests of U 3Si 2 pellets was successfully completed. This is reported in Chapter 3. Secondary creep model of U 3Si 2 was developed and implemented in BISON. This is described in Chapter 4. Properties of U 3Si 2 were implemented in BISON. This is described in Chapter 4. A resonant frequency and damping analyzer (RFDA) using impulse excitation technique (IET) was setup,more » tested, and used to analyze U 3Si 2 samples to measure Young’s and Shear Moduli which were then used to calculate the Poisson ratio for U 3Si 2. This is described in Chapter 5. Characterization of U 3Si 2 samples was completed. Samples were prepared and analyzed by XRD, SEM, and optical microscopy. Grain size analysis was conducted on images. SEM with EDS was used to analyze second phase precipitates. Impulse excitation technique was used to determine the Young’s and Shear Moduli of a tile specimen which allowed for the determination of the Poisson ratio. Helium pycnometry and mercury intrusion porosimetry was performed and used with image analysis to determine porosity size distribution. Vickers microindentation characterization method was used to evaluate the mechanical properties of U 3Si 2 including toughness, hardness, and Vickers hardness. Electrical resistivity measurement was done using the four-point probe method. This is reported in Chapter 5.« less

Study and simulation of low rate video coding schemes

NASA Technical Reports Server (NTRS)

Sayood, Khalid; Chen, Yun-Chung; Kipp, G.

1992-01-01

The semiannual report is included. Topics covered include communication, information science, data compression, remote sensing, color mapped images, robust coding scheme for packet video, recursively indexed differential pulse code modulation, image compression technique for use on token ring networks, and joint source/channel coder design.

Electron-Impact Cross Sections for Ground State to np Excitations of Sodium and Potassium.

PubMed

Stone, Philip M; Kim, Yong-Ki

2004-01-01

Cross sections for electron impact excitation of atoms are important for modeling of low temperature plasmas and gases. While there are many experimental and theoretical results for excitation to the first excited states, little information is available for excitation to higher states. We present here calculations of excitations from the ground state to the np levels of sodium (n = 3 through 11) and potassium (n = 4 through 12). We also present a calculation for a transition from the excited sodium level 3p to 3d to show the generality of the method. Scaling formulas developed earlier by Kim [Phys. Rev. A 64, 032713 (2001)] for plane-wave Born cross sections are used. These formulas have been shown to be remarkably accurate yet simple to use. We have used a core polarization potential in a Dirac-Fock wave function code to calculate target atom wave functions and a matching form of the dipole transition operator to calculate oscillator strengths and Born cross sections. The scaled Born results here for excitation to the first excited levels are in very good agreement with experimental and other theoretical data, and the results for excitation to the next few levels are in satisfactory agreement with the limited data available. The present results for excitation to the higher levels are believed to be the only data available.

Gapped pulses for frequency-swept MRI

NASA Astrophysics Data System (ADS)

Idiyatullin, Djaudat; Corum, Curt; Moeller, Steen; Garwood, Michael

2008-08-01

A recently introduced method called SWIFT (SWeep Imaging with Fourier Transform) is a fundamentally different approach to MRI which is particularly well suited to imaging objects with extremely fast spin-spin relaxation rates. The method exploits a frequency-swept excitation pulse and virtually simultaneous signal acquisition in a time-shared mode. Correlation of the spin system response with the excitation pulse function is used to extract the signals of interest. With SWIFT, image quality is highly dependent on producing uniform and broadband spin excitation. These requirements are satisfied by using frequency-modulated pulses belonging to the hyperbolic secant family (HS n pulses). This article describes the experimental steps needed to properly implement HS n pulses in SWIFT. In addition, properties of HS n pulses in the rapid passage, linear region are investigated, followed by an analysis of the pulses after inserting the "gaps" needed for time-shared excitation and acquisition. Finally, compact expressions are presented to estimate the amplitude and flip angle of the HS n pulses, as well as the relative energy deposited by the SWIFT sequence.

Application of novel low-intensity nonscanning fluorescence lifetime imaging microscopy for monitoring excited state dynamics in individual chloroplasts and living cells of photosynthetic organisms

NASA Astrophysics Data System (ADS)

Eckert, Hann-Jörg; Petrášek, Zdeněk; Kemnitz, Klaus

2006-10-01

Picosecond fluorescence lifetime imaging microscopy (FLIM) provides a most valuable tool to analyze the primary processes of photosynthesis in individual cells and chloroplasts of living cells. In order to obtain correct lifetimes of the excited states, the peak intensity of the exciting laser pulses as well as the average intensity has to be sufficiently low to avoid distortions of the kinetics by processes such as singlet-singlet annihilation, closing of the reaction centers or photoinhibition. In the present study this requirement is achieved by non-scanning wide-field FLIM based on time- and space-correlated single-photon counting (TSCSPC) using a novel microchannel plate photomultiplier with quadrant anode (QA-MCP) that allows parallel acquisition of time-resolved images under minimally invasive low-excitation conditions. The potential of the wide-field TCSPC method is demonstrated by presenting results obtained from measurements of the fluorescence dynamics in individual chloroplasts of moss leaves and living cells of the chlorophyll d-containing cyanobacterium Acaryochloris marina.

In vivo time-gated fluorescence imaging with biodegradable luminescent porous silicon nanoparticles.

PubMed

Gu, Luo; Hall, David J; Qin, Zhengtao; Anglin, Emily; Joo, Jinmyoung; Mooney, David J; Howell, Stephen B; Sailor, Michael J

2013-01-01

Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5-13 μs) of photoluminescent porous silicon nanoparticles can allow the time-gated imaging of tissues in vivo, completely eliminating shorter-lived (<10 ns) emission signals from organic chromophores or tissue autofluorescence. Here using a conventional animal imaging system not optimized for such long-lived excited states, we demonstrate improvement of signal to background contrast ratio by >50-fold in vitro and by >20-fold in vivo when imaging porous silicon nanoparticles. Time-gated imaging of porous silicon nanoparticles accumulated in a human ovarian cancer xenograft following intravenous injection is demonstrated in a live mouse. The potential for multiplexing of images in the time domain by using separate porous silicon nanoparticles engineered with different excited state lifetimes is discussed.

Lensless Photoluminescence Hyperspectral Camera Employing Random Speckle Patterns.

PubMed

Žídek, Karel; Denk, Ondřej; Hlubuček, Jiří

2017-11-10

We propose and demonstrate a spectrally-resolved photoluminescence imaging setup based on the so-called single pixel camera - a technique of compressive sensing, which enables imaging by using a single-pixel photodetector. The method relies on encoding an image by a series of random patterns. In our approach, the image encoding was maintained via laser speckle patterns generated by an excitation laser beam scattered on a diffusor. By using a spectrometer as the single-pixel detector we attained a realization of a spectrally-resolved photoluminescence camera with unmatched simplicity. We present reconstructed hyperspectral images of several model scenes. We also discuss parameters affecting the imaging quality, such as the correlation degree of speckle patterns, pattern fineness, and number of datapoints. Finally, we compare the presented technique to hyperspectral imaging using sample scanning. The presented method enables photoluminescence imaging for a broad range of coherent excitation sources and detection spectral areas.

Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores.

PubMed

Chen, Zhixing; Wei, Lu; Zhu, Xinxin; Min, Wei

2012-08-13

It is highly desirable to be able to optically probe biological activities deep inside live organisms. By employing a spatially confined excitation via a nonlinear transition, multiphoton fluorescence microscopy has become indispensable for imaging scattering samples. However, as the incident laser power drops exponentially with imaging depth due to scattering loss, the out-of-focus fluorescence eventually overwhelms the in-focal signal. The resulting loss of imaging contrast defines a fundamental imaging-depth limit, which cannot be overcome by increasing excitation intensity. Herein we propose to significantly extend this depth limit by multiphoton activation and imaging (MPAI) of photo-activatable fluorophores. The imaging contrast is drastically improved due to the created disparity of bright-dark quantum states in space. We demonstrate this new principle by both analytical theory and experiments on tissue phantoms labeled with synthetic caged fluorescein dye or genetically encodable photoactivatable GFP.

Imaging-guided two-photon excitation-emission-matrix measurements of human skin tissues

NASA Astrophysics Data System (ADS)

Yu, Yingqiu; Lee, Anthony M. D.; Wang, Hequn; Tang, Shuo; Zhao, Jianhua; Lui, Harvey; Zeng, Haishan

2012-07-01

There are increased interests on using multiphoton imaging and spectroscopy for skin tissue characterization and diagnosis. However, most studies have been done with just a few excitation wavelengths. Our objective is to perform a systematic study of the two-photon fluorescence (TPF) properties of skin fluorophores, normal skin, and diseased skin tissues. A nonlinear excitation-emission-matrix (EEM) spectroscopy system with multiphoton imaging guidance was constructed. A tunable femtosecond laser was used to vary excitation wavelengths from 730 to 920 nm for EEM data acquisition. EEM measurements were performed on excised fresh normal skin tissues, seborrheic keratosis tissue samples, and skin fluorophores including: NADH, FAD, keratin, melanin, collagen, and elastin. We found that in the stratum corneum and upper epidermis of normal skin, the cells have large sizes and the TPF originates from keratin. In the lower epidermis, cells are smaller and TPF is dominated by NADH contributions. In the dermis, TPF is dominated by elastin components. The depth resolved EEM measurements also demonstrated that keratin structure has intruded into the middle sublayers of the epidermal part of the seborrheic keratosis lesion. These results suggest that the imaging guided TPF EEM spectroscopy provides useful information for the development of multiphoton clinical devices for skin disease diagnosis.

Images multiplexing by code division technique

NASA Astrophysics Data System (ADS)

Kuo, Chung J.; Rigas, Harriett

Spread Spectrum System (SSS) or Code Division Multiple Access System (CDMAS) has been studied for a long time, but most of the attention was focused on the transmission problems. In this paper, we study the results when the code division technique is applied to the image at the source stage. The idea is to convolve the N different images with the corresponding m-sequence to obtain the encrypted image. The superimposed image (summation of the encrypted images) is then stored or transmitted. The benefit of this is that no one knows what is stored or transmitted unless the m-sequence is known. The recovery of the original image is recovered by correlating the superimposed image with corresponding m-sequence. Two cases are studied in this paper. First, the two-dimensional image is treated as a long one-dimensional vector and the m-sequence is employed to obtain the results. Secondly, the two-dimensional quasi m-array is proposed and used for the code division multiplexing. It is shown that quasi m-array is faster when the image size is 256 x 256. The important features of the proposed technique are not only the image security but also the data compactness. The compression ratio depends on how many images are superimposed.

Images Multiplexing By Code Division Technique

NASA Astrophysics Data System (ADS)

Kuo, Chung Jung; Rigas, Harriett B.

1990-01-01

Spread Spectrum System (SSS) or Code Division Multiple Access System (CDMAS) has been studied for a long time, but most of the attention was focused on the transmission problems. In this paper, we study the results when the code division technique is applied to the image at the source stage. The idea is to convolve the N different images with the corresponding m-sequence to obtain the encrypted image. The superimposed image (summation of the encrypted images) is then stored or transmitted. The benefit of this is that no one knows what is stored or transmitted unless the m-sequence is known. The recovery of the original image is recovered by correlating the superimposed image with corresponding m-sequence. Two cases are studied in this paper. First, the 2-D image is treated as a long 1-D vector and the m-sequence is employed to obtained the results. Secondly, the 2-D quasi m-array is proposed and used for the code division multiplexing. It is showed that quasi m-array is faster when the image size is 256x256. The important features of the proposed technique are not only the image security but also the data compactness. The compression ratio depends on how many images are superimposed.

A source-channel coding approach to digital image protection and self-recovery.

PubMed

Sarreshtedari, Saeed; Akhaee, Mohammad Ali

2015-07-01

Watermarking algorithms have been widely applied to the field of image forensics recently. One of these very forensic applications is the protection of images against tampering. For this purpose, we need to design a watermarking algorithm fulfilling two purposes in case of image tampering: 1) detecting the tampered area of the received image and 2) recovering the lost information in the tampered zones. State-of-the-art techniques accomplish these tasks using watermarks consisting of check bits and reference bits. Check bits are used for tampering detection, whereas reference bits carry information about the whole image. The problem of recovering the lost reference bits still stands. This paper is aimed at showing that having the tampering location known, image tampering can be modeled and dealt with as an erasure error. Therefore, an appropriate design of channel code can protect the reference bits against tampering. In the present proposed method, the total watermark bit-budget is dedicated to three groups: 1) source encoder output bits; 2) channel code parity bits; and 3) check bits. In watermark embedding phase, the original image is source coded and the output bit stream is protected using appropriate channel encoder. For image recovery, erasure locations detected by check bits help channel erasure decoder to retrieve the original source encoded image. Experimental results show that our proposed scheme significantly outperforms recent techniques in terms of image quality for both watermarked and recovered image. The watermarked image quality gain is achieved through spending less bit-budget on watermark, while image recovery quality is considerably improved as a consequence of consistent performance of designed source and channel codes.

Accessible and informative sectioned images, color-coded images, and surface models of the ear.

PubMed

Park, Hyo Seok; Chung, Min Suk; Shin, Dong Sun; Jung, Yong Wook; Park, Jin Seo

2013-08-01

In our previous research, we created state-of-the-art sectioned images, color-coded images, and surface models of the human ear. Our ear data would be more beneficial and informative if they were more easily accessible. Therefore, the purpose of this study was to distribute the browsing software and the PDF file in which ear images are to be readily obtainable and freely explored. Another goal was to inform other researchers of our methods for establishing the browsing software and the PDF file. To achieve this, sectioned images and color-coded images of ear were prepared (voxel size 0.1 mm). In the color-coded images, structures related to hearing, equilibrium, and structures originated from the first and second pharyngeal arches were segmented supplementarily. The sectioned and color-coded images of right ear were added to the browsing software, which displayed the images serially along with structure names. The surface models were reconstructed to be combined into the PDF file where they could be freely manipulated. Using the browsing software and PDF file, sectional and three-dimensional shapes of ear structures could be comprehended in detail. Furthermore, using the PDF file, clinical knowledge could be identified through virtual otoscopy. Therefore, the presented educational tools will be helpful to medical students and otologists by improving their knowledge of ear anatomy. The browsing software and PDF file can be downloaded without charge and registration at our homepage (http://anatomy.dongguk.ac.kr/ear/). Copyright © 2013 Wiley Periodicals, Inc.

Stimulated Raman scattering: old physics, new applications.

PubMed

Yakovlev, Vladislav V; Petrov, Georgi I; Zhang, Hao F; Noojin, Gary D; Denton, Michael L; Thomas, Robert J; Scully, Marlan O

2009-10-01

Stimulated Raman scattering as a promising way of expanding the tunability of ultrafast lasers and as an exciting new biomedical imaging modality capable of selective excitation and chemically-specific diagnostics of molecular species.

Feasibility for detection of autofluorescent signatures in rat organs using a novel excitation-scanning hyperspectral imaging system

NASA Astrophysics Data System (ADS)

Favreau, Peter F.; Deal, Joshua A.; Weber, David S.; Rich, Thomas C.; Leavesley, Silas J.

2016-04-01

The natural fluorescence (autofluorescence) of tissues has been noted as a biomarker for cancer for several decades. Autofluorescence contrast between tumors and healthy tissues has been of significant interest in endoscopy, leading to development of autofluorescence endoscopes capable of visualizing 2-3 fluorescence emission wavelengths to achieve maximal contrast. However, tumor detection with autofluorescence endoscopes is hindered by low fluorescence signal and limited quantitative information, resulting in prolonged endoscopic procedures, prohibitive acquisition times, and reduced specificity of detection. Our lab has designed a novel excitation-scanning hyperspectral imaging system with high fluorescence signal detection, low acquisition time, and enhanced spectral discrimination. In this study, we surveyed a comprehensive set of excised tissues to assess the feasibility of detecting tissue-specific pathologies using excitation-scanning. Fresh, untreated tissue specimens were imaged from 360 to 550 nm on an inverted fluorescence microscope equipped with a set of thin-film tunable filters (Semrock, A Unit of IDEX). Images were subdivided into training and test sets. Automated endmember extraction (ENVI 5.1, Exelis) with PCA identified endmembers within training images of autofluorescence. A spectral library was created from 9 endmembers. The library was used for identification of endmembers in test images. Our results suggest (1) spectral differentiation of multiple tissue types is possible using excitation scanning; (2) shared spectra between tissue types; and (3) the ability to identify unique morphological features in disparate tissues from shared autofluorescent components. Future work will focus on isolating specific molecular signatures present in tissue spectra, and elucidating the contribution of these signatures in pathologies.

Contact resonance atomic force microscopy imaging in air and water using photothermal excitation.

PubMed

Kocun, Marta; Labuda, Aleksander; Gannepalli, Anil; Proksch, Roger

2015-08-01

Contact Resonance Force Microscopy (CR-FM) is a leading atomic force microscopy technique for measuring viscoelastic nano-mechanical properties. Conventional piezo-excited CR-FM measurements have been limited to imaging in air, since the "forest of peaks" frequency response associated with acoustic excitation methods effectively masks the true cantilever resonance. Using photothermal excitation results in clean contact, resonance spectra that closely match the ideal frequency response of the cantilever, allowing unambiguous and simple resonance frequency and quality factor measurements in air and liquids alike. This extends the capabilities of CR-FM to biologically relevant and other soft samples in liquid environments. We demonstrate CR-FM in air and water on both stiff silicon/titanium samples and softer polystyrene-polyethylene-polypropylene polymer samples with the quantitative moduli having very good agreement between expected and measured values.

Lifetimes and energetics of the first electronically excited states of NaH2O from time-resolved photoelectron imaging

NASA Astrophysics Data System (ADS)

Gartmann, Thomas E.; Yoder, Bruce L.; Chasovskikh, Egor; Signorell, Ruth

2017-09-01

The energetics and lifetimes of the first electronically excited states (;3p-states;) of NaH2O and NaD2O have been measured by pump-probe (740/780 and 400 nm) photoelectron imaging. The photoelectron spectra of NaH2O show two bands at an electron kinetic energy of 0.14 and 0.38 eV, respectively. We assign the former to excitation via the two energetically close lying ;pπ-states; with flat potential curves in the intermolecular degrees of freedom, and the latter to the excitation via the ;pσ-state; characterized by significantly steeper potential curves. The relaxation of all ;p-states; follows a double exponential decay with a lifetime around 110 ps for the dominant fast component.

A novel data processing technique for image reconstruction of penumbral imaging

NASA Astrophysics Data System (ADS)

Xie, Hongwei; Li, Hongyun; Xu, Zeping; Song, Guzhou; Zhang, Faqiang; Zhou, Lin

2011-06-01

CT image reconstruction technique was applied to the data processing of the penumbral imaging. Compared with other traditional processing techniques for penumbral coded pinhole image such as Wiener, Lucy-Richardson and blind technique, this approach is brand new. In this method, the coded aperture processing method was used for the first time independent to the point spread function of the image diagnostic system. In this way, the technical obstacles was overcome in the traditional coded pinhole image processing caused by the uncertainty of point spread function of the image diagnostic system. Then based on the theoretical study, the simulation of penumbral imaging and image reconstruction was carried out to provide fairly good results. While in the visible light experiment, the point source of light was used to irradiate a 5mm×5mm object after diffuse scattering and volume scattering. The penumbral imaging was made with aperture size of ~20mm. Finally, the CT image reconstruction technique was used for image reconstruction to provide a fairly good reconstruction result.

Ensemble coding of face identity is present but weaker in congenital prosopagnosia.

PubMed

Robson, Matthew K; Palermo, Romina; Jeffery, Linda; Neumann, Markus F

2018-03-01

Individuals with congenital prosopagnosia (CP) are impaired at identifying individual faces but do not appear to show impairments in extracting the average identity from a group of faces (known as ensemble coding). However, possible deficits in ensemble coding in a previous study (CPs n = 4) may have been masked because CPs relied on pictorial (image) cues rather than identity cues. Here we asked whether a larger sample of CPs (n = 11) would show intact ensemble coding of identity when availability of image cues was minimised. Participants viewed a "set" of four faces and then judged whether a subsequent individual test face, either an exemplar or a "set average", was in the preceding set. Ensemble coding occurred when matching (vs. mismatching) averages were mistakenly endorsed as set members. We assessed both image- and identity-based ensemble coding, by varying whether test faces were either the same or different images of the identities in the set. CPs showed significant ensemble coding in both tasks, indicating that their performance was independent of image cues. As a group, CPs' ensemble coding was weaker than controls in both tasks, consistent with evidence that perceptual processing of face identity is disrupted in CP. This effect was driven by CPs (n= 3) who, in addition to having impaired face memory, also performed particularly poorly on a measure of face perception (CFPT). Future research, using larger samples, should examine whether deficits in ensemble coding may be restricted to CPs who also have substantial face perception deficits. Copyright © 2018 Elsevier Ltd. All rights reserved.

Image Coding Based on Address Vector Quantization.

NASA Astrophysics Data System (ADS)

Feng, Yushu

Image coding is finding increased application in teleconferencing, archiving, and remote sensing. This thesis investigates the potential of Vector Quantization (VQ), a relatively new source coding technique, for compression of monochromatic and color images. Extensions of the Vector Quantization technique to the Address Vector Quantization method have been investigated. In Vector Quantization, the image data to be encoded are first processed to yield a set of vectors. A codeword from the codebook which best matches the input image vector is then selected. Compression is achieved by replacing the image vector with the index of the code-word which produced the best match, the index is sent to the channel. Reconstruction of the image is done by using a table lookup technique, where the label is simply used as an address for a table containing the representative vectors. A code-book of representative vectors (codewords) is generated using an iterative clustering algorithm such as K-means, or the generalized Lloyd algorithm. A review of different Vector Quantization techniques are given in chapter 1. Chapter 2 gives an overview of codebook design methods including the Kohonen neural network to design codebook. During the encoding process, the correlation of the address is considered and Address Vector Quantization is developed for color image and monochrome image coding. Address VQ which includes static and dynamic processes is introduced in chapter 3. In order to overcome the problems in Hierarchical VQ, Multi-layer Address Vector Quantization is proposed in chapter 4. This approach gives the same performance as that of the normal VQ scheme but the bit rate is about 1/2 to 1/3 as that of the normal VQ method. In chapter 5, a Dynamic Finite State VQ based on a probability transition matrix to select the best subcodebook to encode the image is developed. In chapter 6, a new adaptive vector quantization scheme, suitable for color video coding, called "A Self -Organizing Adaptive VQ Technique" is presented. In addition to chapters 2 through 6 which report on new work, this dissertation includes one chapter (chapter 1) and part of chapter 2 which review previous work on VQ and image coding, respectively. Finally, a short discussion of directions for further research is presented in conclusion.

Extreme ultraviolet emission spectra of Gd and Tb ions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kilbane, D.; O'Sullivan, G.

2010-11-15

Theoretical extreme ultraviolet emission spectra of gadolinium and terbium ions calculated with the Cowan suite of codes and the flexible atomic code (FAC) relativistic code are presented. 4d-4f and 4p-4d transitions give rise to unresolved transition arrays in a range of ions. The effects of configuration interaction are investigated for transitions between singly excited configurations. Optimization of emission at 6.775 nm and 6.515 nm is achieved for Gd and Tb ions, respectively, by consideration of plasma effects. The resulting synthetic spectra are compared with experimental spectra recorded using the laser produced plasma technique.

A joint source-channel distortion model for JPEG compressed images.

PubMed

Sabir, Muhammad F; Sheikh, Hamid Rahim; Heath, Robert W; Bovik, Alan C

2006-06-01

The need for efficient joint source-channel coding (JSCC) is growing as new multimedia services are introduced in commercial wireless communication systems. An important component of practical JSCC schemes is a distortion model that can predict the quality of compressed digital multimedia such as images and videos. The usual approach in the JSCC literature for quantifying the distortion due to quantization and channel errors is to estimate it for each image using the statistics of the image for a given signal-to-noise ratio (SNR). This is not an efficient approach in the design of real-time systems because of the computational complexity. A more useful and practical approach would be to design JSCC techniques that minimize average distortion for a large set of images based on some distortion model rather than carrying out per-image optimizations. However, models for estimating average distortion due to quantization and channel bit errors in a combined fashion for a large set of images are not available for practical image or video coding standards employing entropy coding and differential coding. This paper presents a statistical model for estimating the distortion introduced in progressive JPEG compressed images due to quantization and channel bit errors in a joint manner. Statistical modeling of important compression techniques such as Huffman coding, differential pulse-coding modulation, and run-length coding are included in the model. Examples show that the distortion in terms of peak signal-to-noise ratio (PSNR) can be predicted within a 2-dB maximum error over a variety of compression ratios and bit-error rates. To illustrate the utility of the proposed model, we present an unequal power allocation scheme as a simple application of our model. Results show that it gives a PSNR gain of around 6.5 dB at low SNRs, as compared to equal power allocation.

Fast Scattering Code (FSC) User's Manual: Version 2

NASA Technical Reports Server (NTRS)

Tinetti, Ana F.; Dun, M. H.; Pope, D. Stuart

2006-01-01

The Fast Scattering Code (version 2.0) is a computer program for predicting the three-dimensional scattered acoustic field produced by the interaction of known, time-harmonic, incident sound with aerostructures in the presence of potential background flow. The FSC has been developed for use as an aeroacoustic analysis tool for assessing global effects on noise radiation and scattering caused by changes in configuration (geometry, component placement) and operating conditions (background flow, excitation frequency).

Multiphoton Microscopy of Prostate and Periprostatic Neural Tissue: A Promising Imaging Technique for Improving Nerve-Sparing Prostatectomy

PubMed Central

Yadav, Rajiv; Mukherjee, Sushmita; Hermen, Michael; Tan, Gerald; Maxfield, Frederick R.; Webb, Watt W.

2009-01-01

Abstract Background and Purpose Various imaging modalities are under investigation for real-time tissue imaging of periprostatic nerves with the idea of improving the results of nerve-sparing radical prostatectomy. We explored multiphoton microscopy (MPM) for real-time tissue imaging of the prostate and periprostatic neural tissue in a male Sprague-Dawley rat model. The unique advantage of this technique is the acquisition of high-resolution images without necessitating any extrinsic labeling agent and with minimal phototoxic effect on tissue. Materials and Methods The prostate and cavernous nerves were surgically excised from male Sprague-Dawley rats. The imaging was carried out using intrinsic fluorescence and scattering properties of the tissues without any exogenous dye or contrast agent. A custom-built MPM, consisting of an Olympus BX61WI upright frame and a modified MRC 1024 scanhead, was used. A femtosecond pulsed titanium/sapphire laser at 780-nm wavelength was used to excite the tissue; laser power under the objective was modulated via a Pockels cell. Second harmonic generation (SHG) signals were collected at 390 (±35 nm), and broadband autofluorescence was collected at 380 to 530 nm. The images obtained from SHG and from tissue fluorescence were then merged and color coded during postprocessing for better appreciation of details. The corresponding tissues were subjected to hematoxylin and eosin staining for histologic confirmation of the structures. Results High-resolution images of the prostate capsule, underlying acini, and individual cells outlining the glands were obtained at varying magnifications. MPM images of adipose tissue and the neural tissues were also obtained. Histologic confirmation and correlation of the prostate gland, fat, cavernous nerve, and major pelvic ganglion validated the findings of MPM. Conclusion Real-time imaging and microscopic resolution of prostate and periprostatic neural tissue using MPM is feasible without the need for any extrinsic labeling agents. Integration of this imaging modality with operative technique has the potential to improve the precision of nerve-sparing prostatectomy. PMID:19425823

Weighted bi-prediction for light field image coding

NASA Astrophysics Data System (ADS)

Conti, Caroline; Nunes, Paulo; Ducla Soares, Luís.

2017-09-01

Light field imaging based on a single-tier camera equipped with a microlens array - also known as integral, holoscopic, and plenoptic imaging - has currently risen up as a practical and prospective approach for future visual applications and services. However, successfully deploying actual light field imaging applications and services will require developing adequate coding solutions to efficiently handle the massive amount of data involved in these systems. In this context, self-similarity compensated prediction is a non-local spatial prediction scheme based on block matching that has been shown to achieve high efficiency for light field image coding based on the High Efficiency Video Coding (HEVC) standard. As previously shown by the authors, this is possible by simply averaging two predictor blocks that are jointly estimated from a causal search window in the current frame itself, referred to as self-similarity bi-prediction. However, theoretical analyses for motion compensated bi-prediction have suggested that it is still possible to achieve further rate-distortion performance improvements by adaptively estimating the weighting coefficients of the two predictor blocks. Therefore, this paper presents a comprehensive study of the rate-distortion performance for HEVC-based light field image coding when using different sets of weighting coefficients for self-similarity bi-prediction. Experimental results demonstrate that it is possible to extend the previous theoretical conclusions to light field image coding and show that the proposed adaptive weighting coefficient selection leads to up to 5 % of bit savings compared to the previous self-similarity bi-prediction scheme.

Design and implementation of coded aperture coherent scatter spectral imaging of cancerous and healthy breast tissue samples

PubMed Central

Lakshmanan, Manu N.; Greenberg, Joel A.; Samei, Ehsan; Kapadia, Anuj J.

2016-01-01

Abstract. A scatter imaging technique for the differentiation of cancerous and healthy breast tissue in a heterogeneous sample is introduced in this work. Such a technique has potential utility in intraoperative margin assessment during lumpectomy procedures. In this work, we investigate the feasibility of the imaging method for tumor classification using Monte Carlo simulations and physical experiments. The coded aperture coherent scatter spectral imaging technique was used to reconstruct three-dimensional (3-D) images of breast tissue samples acquired through a single-position snapshot acquisition, without rotation as is required in coherent scatter computed tomography. We perform a quantitative assessment of the accuracy of the cancerous voxel classification using Monte Carlo simulations of the imaging system; describe our experimental implementation of coded aperture scatter imaging; show the reconstructed images of the breast tissue samples; and present segmentations of the 3-D images in order to identify the cancerous and healthy tissue in the samples. From the Monte Carlo simulations, we find that coded aperture scatter imaging is able to reconstruct images of the samples and identify the distribution of cancerous and healthy tissues (i.e., fibroglandular, adipose, or a mix of the two) inside them with a cancerous voxel identification sensitivity, specificity, and accuracy of 92.4%, 91.9%, and 92.0%, respectively. From the experimental results, we find that the technique is able to identify cancerous and healthy tissue samples and reconstruct differential coherent scatter cross sections that are highly correlated with those measured by other groups using x-ray diffraction. Coded aperture scatter imaging has the potential to provide scatter images that automatically differentiate cancerous and healthy tissue inside samples within a time on the order of a minute per slice. PMID:26962543

Design and implementation of coded aperture coherent scatter spectral imaging of cancerous and healthy breast tissue samples.

PubMed

Lakshmanan, Manu N; Greenberg, Joel A; Samei, Ehsan; Kapadia, Anuj J

2016-01-01

A scatter imaging technique for the differentiation of cancerous and healthy breast tissue in a heterogeneous sample is introduced in this work. Such a technique has potential utility in intraoperative margin assessment during lumpectomy procedures. In this work, we investigate the feasibility of the imaging method for tumor classification using Monte Carlo simulations and physical experiments. The coded aperture coherent scatter spectral imaging technique was used to reconstruct three-dimensional (3-D) images of breast tissue samples acquired through a single-position snapshot acquisition, without rotation as is required in coherent scatter computed tomography. We perform a quantitative assessment of the accuracy of the cancerous voxel classification using Monte Carlo simulations of the imaging system; describe our experimental implementation of coded aperture scatter imaging; show the reconstructed images of the breast tissue samples; and present segmentations of the 3-D images in order to identify the cancerous and healthy tissue in the samples. From the Monte Carlo simulations, we find that coded aperture scatter imaging is able to reconstruct images of the samples and identify the distribution of cancerous and healthy tissues (i.e., fibroglandular, adipose, or a mix of the two) inside them with a cancerous voxel identification sensitivity, specificity, and accuracy of 92.4%, 91.9%, and 92.0%, respectively. From the experimental results, we find that the technique is able to identify cancerous and healthy tissue samples and reconstruct differential coherent scatter cross sections that are highly correlated with those measured by other groups using x-ray diffraction. Coded aperture scatter imaging has the potential to provide scatter images that automatically differentiate cancerous and healthy tissue inside samples within a time on the order of a minute per slice.

Information theoretical assessment of digital imaging systems

NASA Technical Reports Server (NTRS)

John, Sarah; Rahman, Zia-Ur; Huck, Friedrich O.; Reichenbach, Stephen E.

1990-01-01

The end-to-end performance of image gathering, coding, and restoration as a whole is considered. This approach is based on the pivotal relationship that exists between the spectral information density of the transmitted signal and the restorability of images from this signal. The information-theoretical assessment accounts for (1) the information density and efficiency of the acquired signal as a function of the image-gathering system design and the radiance-field statistics, and (2) the improvement in information efficiency and data compression that can be gained by combining image gathering with coding to reduce the signal redundancy and irrelevancy. It is concluded that images can be restored with better quality and from fewer data as the information efficiency of the data is increased. The restoration correctly explains the image gathering and coding processes and effectively suppresses the image-display degradations.

Information theoretical assessment of digital imaging systems

NASA Astrophysics Data System (ADS)

John, Sarah; Rahman, Zia-Ur; Huck, Friedrich O.; Reichenbach, Stephen E.

1990-10-01

The end-to-end performance of image gathering, coding, and restoration as a whole is considered. This approach is based on the pivotal relationship that exists between the spectral information density of the transmitted signal and the restorability of images from this signal. The information-theoretical assessment accounts for (1) the information density and efficiency of the acquired signal as a function of the image-gathering system design and the radiance-field statistics, and (2) the improvement in information efficiency and data compression that can be gained by combining image gathering with coding to reduce the signal redundancy and irrelevancy. It is concluded that images can be restored with better quality and from fewer data as the information efficiency of the data is increased. The restoration correctly explains the image gathering and coding processes and effectively suppresses the image-display degradations.

An adaptive technique to maximize lossless image data compression of satellite images

NASA Technical Reports Server (NTRS)

Stewart, Robert J.; Lure, Y. M. Fleming; Liou, C. S. Joe

1994-01-01

Data compression will pay an increasingly important role in the storage and transmission of image data within NASA science programs as the Earth Observing System comes into operation. It is important that the science data be preserved at the fidelity the instrument and the satellite communication systems were designed to produce. Lossless compression must therefore be applied, at least, to archive the processed instrument data. In this paper, we present an analysis of the performance of lossless compression techniques and develop an adaptive approach which applied image remapping, feature-based image segmentation to determine regions of similar entropy and high-order arithmetic coding to obtain significant improvements over the use of conventional compression techniques alone. Image remapping is used to transform the original image into a lower entropy state. Several techniques were tested on satellite images including differential pulse code modulation, bi-linear interpolation, and block-based linear predictive coding. The results of these experiments are discussed and trade-offs between computation requirements and entropy reductions are used to identify the optimum approach for a variety of satellite images. Further entropy reduction can be achieved by segmenting the image based on local entropy properties then applying a coding technique which maximizes compression for the region. Experimental results are presented showing the effect of different coding techniques for regions of different entropy. A rule-base is developed through which the technique giving the best compression is selected. The paper concludes that maximum compression can be achieved cost effectively and at acceptable performance rates with a combination of techniques which are selected based on image contextual information.

Adaptive coded aperture imaging in the infrared: towards a practical implementation

NASA Astrophysics Data System (ADS)

Slinger, Chris W.; Gilholm, Kevin; Gordon, Neil; McNie, Mark; Payne, Doug; Ridley, Kevin; Strens, Malcolm; Todd, Mike; De Villiers, Geoff; Watson, Philip; Wilson, Rebecca; Dyer, Gavin; Eismann, Mike; Meola, Joe; Rogers, Stanley

2008-08-01

An earlier paper [1] discussed the merits of adaptive coded apertures for use as lensless imaging systems in the thermal infrared and visible. It was shown how diffractive (rather than the more conventional geometric) coding could be used, and that 2D intensity measurements from multiple mask patterns could be combined and decoded to yield enhanced imagery. Initial experimental results in the visible band were presented. Unfortunately, radiosity calculations, also presented in that paper, indicated that the signal to noise performance of systems using this approach was likely to be compromised, especially in the infrared. This paper will discuss how such limitations can be overcome, and some of the tradeoffs involved. Experimental results showing tracking and imaging performance of these modified, diffractive, adaptive coded aperture systems in the visible and infrared will be presented. The subpixel imaging and tracking performance is compared to that of conventional imaging systems and shown to be superior. System size, weight and cost calculations indicate that the coded aperture approach, employing novel photonic MOEMS micro-shutter architectures, has significant merits for a given level of performance in the MWIR when compared to more conventional imaging approaches.

Structured codebook design in CELP

NASA Technical Reports Server (NTRS)

Leblanc, W. P.; Mahmoud, S. A.

1990-01-01

Codebook Excited Linear Protection (CELP) is a popular analysis by synthesis technique for quantizing speech at bit rates from 4 to 6 kbps. Codebook design techniques to date have been largely based on either random (often Gaussian) codebooks, or on known binary or ternary codes which efficiently map the space of (assumed white) excitation codevectors. It has been shown that by introducing symmetries into the codebook, good complexity reduction can be realized with only marginal decrease in performance. Codebook design algorithms are considered for a wide range of structured codebooks.

Comparison of ATLOG and Xyce for Bell Labs Electromagnetic Pulse Excitation of Finite-Long Dissipative Conductors over a Ground Plane.

DOE Office of Scientific and Technical Information (OSTI.GOV)

campione, Salvatore; Warne, Larry K.; Schiek, Richard

This report details the modeling results for the response of a finite-length dissipative conductor interacting with a conducting ground to the Bell Labs electromagnetic pulse excitation. We use both a frequency-domain and a time-domain method based on transmission line theory through a code we call ATLOG - Analytic Transmission Line Over Ground. Results are compared to the circuit simulator Xyce for selected cases. Intentionally Left Blank

A variable rate speech compressor for mobile applications

NASA Technical Reports Server (NTRS)

Yeldener, S.; Kondoz, A. M.; Evans, B. G.

1990-01-01

One of the most promising speech coder at the bit rate of 9.6 to 4.8 kbits/s is CELP. Code Excited Linear Prediction (CELP) has been dominating 9.6 to 4.8 kbits/s region during the past 3 to 4 years. Its set back however, is its expensive implementation. As an alternative to CELP, the Base-Band CELP (CELP-BB) was developed which produced good quality speech comparable to CELP and a single chip implementable complexity as reported previously. Its robustness was also improved to tolerate errors up to 1.0 pct. and maintain intelligibility up to 5.0 pct. and more. Although, CELP-BB produces good quality speech at around 4.8 kbits/s, it has a fundamental problem when updating the pitch filter memory. A sub-optimal solution is proposed for this problem. Below 4.8 kbits/s, however, CELP-BB suffers from noticeable quantization noise as a result of the large vector dimensions used. Efficient representation of speech below 4.8 kbits/s is reported by introducing Sinusoidal Transform Coding (STC) to represent the LPC excitation which is called Sine Wave Excited LPC (SWELP). In this case, natural sounding good quality synthetic speech is obtained at around 2.4 kbits/s.

Information theoretical assessment of image gathering and coding for digital restoration

NASA Technical Reports Server (NTRS)

Huck, Friedrich O.; John, Sarah; Reichenbach, Stephen E.

1990-01-01

The process of image-gathering, coding, and restoration is presently treated in its entirety rather than as a catenation of isolated tasks, on the basis of the relationship between the spectral information density of a transmitted signal and the restorability of images from the signal. This 'information-theoretic' assessment accounts for the information density and efficiency of the acquired signal as a function of the image-gathering system's design and radiance-field statistics, as well as for the information efficiency and data compression that are obtainable through the combination of image gathering with coding to reduce signal redundancy. It is found that high information efficiency is achievable only through minimization of image-gathering degradation as well as signal redundancy.

A Lossless hybrid wavelet-fractal compression for welding radiographic images.

PubMed

Mekhalfa, Faiza; Avanaki, Mohammad R N; Berkani, Daoud

2016-01-01

In this work a lossless wavelet-fractal image coder is proposed. The process starts by compressing and decompressing the original image using wavelet transformation and fractal coding algorithm. The decompressed image is removed from the original one to obtain a residual image which is coded by using Huffman algorithm. Simulation results show that with the proposed scheme, we achieve an infinite peak signal to noise ratio (PSNR) with higher compression ratio compared to typical lossless method. Moreover, the use of wavelet transform speeds up the fractal compression algorithm by reducing the size of the domain pool. The compression results of several welding radiographic images using the proposed scheme are evaluated quantitatively and compared with the results of Huffman coding algorithm.

Coded aperture detector: an image sensor with sub 20-nm pixel resolution.

PubMed

Miyakawa, Ryan; Mayer, Rafael; Wojdyla, Antoine; Vannier, Nicolas; Lesser, Ian; Aron-Dine, Shifrah; Naulleau, Patrick

2014-08-11

We describe the coded aperture detector, a novel image sensor based on uniformly redundant arrays (URAs) with customizable pixel size, resolution, and operating photon energy regime. In this sensor, a coded aperture is scanned laterally at the image plane of an optical system, and the transmitted intensity is measured by a photodiode. The image intensity is then digitally reconstructed using a simple convolution. We present results from a proof-of-principle optical prototype, demonstrating high-fidelity image sensing comparable to a CCD. A 20-nm half-pitch URA fabricated by the Center for X-ray Optics (CXRO) nano-fabrication laboratory is presented that is suitable for high-resolution image sensing at EUV and soft X-ray wavelengths.

Adaptive bit plane quadtree-based block truncation coding for image compression

NASA Astrophysics Data System (ADS)

Li, Shenda; Wang, Jin; Zhu, Qing

2018-04-01

Block truncation coding (BTC) is a fast image compression technique applied in spatial domain. Traditional BTC and its variants mainly focus on reducing computational complexity for low bit rate compression, at the cost of lower quality of decoded images, especially for images with rich texture. To solve this problem, in this paper, a quadtree-based block truncation coding algorithm combined with adaptive bit plane transmission is proposed. First, the direction of edge in each block is detected using Sobel operator. For the block with minimal size, adaptive bit plane is utilized to optimize the BTC, which depends on its MSE loss encoded by absolute moment block truncation coding (AMBTC). Extensive experimental results show that our method gains 0.85 dB PSNR on average compare to some other state-of-the-art BTC variants. So it is desirable for real time image compression applications.

Image gathering, coding, and processing: End-to-end optimization for efficient and robust acquisition of visual information

NASA Technical Reports Server (NTRS)

Huck, Friedrich O.; Fales, Carl L.

1990-01-01

Researchers are concerned with the end-to-end performance of image gathering, coding, and processing. The applications range from high-resolution television to vision-based robotics, wherever the resolution, efficiency and robustness of visual information acquisition and processing are critical. For the presentation at this workshop, it is convenient to divide research activities into the following two overlapping areas: The first is the development of focal-plane processing techniques and technology to effectively combine image gathering with coding, with an emphasis on low-level vision processing akin to the retinal processing in human vision. The approach includes the familiar Laplacian pyramid, the new intensity-dependent spatial summation, and parallel sensing/processing networks. Three-dimensional image gathering is attained by combining laser ranging with sensor-array imaging. The second is the rigorous extension of information theory and optimal filtering to visual information acquisition and processing. The goal is to provide a comprehensive methodology for quantitatively assessing the end-to-end performance of image gathering, coding, and processing.

Permutation coding technique for image recognition systems.

PubMed

Kussul, Ernst M; Baidyk, Tatiana N; Wunsch, Donald C; Makeyev, Oleksandr; Martín, Anabel

2006-11-01

A feature extractor and neural classifier for image recognition systems are proposed. The proposed feature extractor is based on the concept of random local descriptors (RLDs). It is followed by the encoder that is based on the permutation coding technique that allows to take into account not only detected features but also the position of each feature on the image and to make the recognition process invariant to small displacements. The combination of RLDs and permutation coding permits us to obtain a sufficiently general description of the image to be recognized. The code generated by the encoder is used as an input data for the neural classifier. Different types of images were used to test the proposed image recognition system. It was tested in the handwritten digit recognition problem, the face recognition problem, and the microobject shape recognition problem. The results of testing are very promising. The error rate for the Modified National Institute of Standards and Technology (MNIST) database is 0.44% and for the Olivetti Research Laboratory (ORL) database it is 0.1%.

The location and recognition of anti-counterfeiting code image with complex background

NASA Astrophysics Data System (ADS)

Ni, Jing; Liu, Quan; Lou, Ping; Han, Ping

2017-07-01

The order of cigarette market is a key issue in the tobacco business system. The anti-counterfeiting code, as a kind of effective anti-counterfeiting technology, can identify counterfeit goods, and effectively maintain the normal order of market and consumers' rights and interests. There are complex backgrounds, light interference and other problems in the anti-counterfeiting code images obtained by the tobacco recognizer. To solve these problems, the paper proposes a locating method based on Susan operator, combined with sliding window and line scanning,. In order to reduce the interference of background and noise, we extract the red component of the image and convert the color image into gray image. For the confusing characters, recognition results correction based on the template matching method has been adopted to improve the recognition rate. In this method, the anti-counterfeiting code can be located and recognized correctly in the image with complex background. The experiment results show the effectiveness and feasibility of the approach.

Full-field modal analysis during base motion excitation using high-speed 3D digital image correlation

NASA Astrophysics Data System (ADS)

Molina-Viedma, Ángel J.; López-Alba, Elías; Felipe-Sesé, Luis; Díaz, Francisco A.

2017-10-01

In recent years, many efforts have been made to exploit full-field measurement optical techniques for modal identification. Three-dimensional digital image correlation using high-speed cameras has been extensively employed for this purpose. Modal identification algorithms are applied to process the frequency response functions (FRF), which relate the displacement response of the structure to the excitation force. However, one of the most common tests for modal analysis involves the base motion excitation of a structural element instead of force excitation. In this case, the relationship between response and excitation is typically based on displacements, which are known as transmissibility functions. In this study, a methodology for experimental modal analysis using high-speed 3D digital image correlation and base motion excitation tests is proposed. In particular, a cantilever beam was excited from its base with a random signal, using a clamped edge join. Full-field transmissibility functions were obtained through the beam and converted into FRF for proper identification, considering a single degree-of-freedom theoretical conversion. Subsequently, modal identification was performed using a circle-fit approach. The proposed methodology facilitates the management of the typically large amounts of data points involved in the DIC measurement during modal identification. Moreover, it was possible to determine the natural frequencies, damping ratios and full-field mode shapes without requiring any additional tests. Finally, the results were experimentally validated by comparing them with those obtained by employing traditional accelerometers, analytical models and finite element method analyses. The comparison was performed by using the quantitative indicator modal assurance criterion. The results showed a high level of correspondence, consolidating the proposed experimental methodology.

Measurement of excitation functions in alpha induced reactions on natCu

NASA Astrophysics Data System (ADS)

Shahid, Muhammad; Kim, Kwangsoo; Kim, Guinyun; Zaman, Muhammad; Nadeem, Muhammad

2015-09-01

The excitation functions of 66,67,68Ga, 62,63,65Zn, 61,64Cu, and 58,60Co radionuclides in the natCu(α, x) reaction were measured in the energy range from 15 to 42 MeV by using a stacked-foil activation method at the MC-50 cyclotron of the Korean Institute of Radiological and Medical Sciences. The measured results were compared with the literature data as well as the theoretical values obtained from the TENDL-2013 and TENDL-2014 libraries based on the TALYS-1.6 code. The integral yields for thick targets of the produced radionuclides were also determined from the measured excitation functions and the stopping power of natural copper.

Experiment-theory comparison for low frequency BAE modes in the strongly shaped H-1NF stellarator

DOE PAGES

Haskey, S. R.; Blackwell, B. D.; Nuhrenberg, C.; ...

2015-08-12

Here, recent advances in the modeling, analysis, and measurement of fluctuations have significantly improved the diagnosis and understanding of Alfvén eigenmodes in the strongly shaped H-1NF helical axis stellarator. Experimental measurements, including 3D tomographic inversions of high resolution visible light images, are in close agreement with beta-induced Alfvén eigenmodes (BAEs) calculated using the compressible ideal MHD code, CAS3D. This is despite the low β in H-1NF, providing experimental evidence that these modes can exist due to compression that is induced by the strong shaping in stellarators, in addition to high β, as is the case in tokamaks. This is confirmedmore » using the CONTI and CAS3D codes, which show significant gap structures at lower frequencies which contain BAE and beta-acoustic Alfvén eigenmodes (BAAEs). The BAEs are excited in the absence of a well confined energetic particle source, further confirming previous studies that thermal particles, electrons, or even radiation fluctuations can drive these modes. Datamining of magnetic probe data shows the experimentally measured frequency of these modes has a clear dependence on the rotational transform profile, which is consistent with a frequency dependency due to postulated confinement related temperature variations.« less

Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate.

PubMed

Eibl, Matthias; Karpf, Sebastian; Weng, Daniel; Hakert, Hubertus; Pfeiffer, Tom; Kolb, Jan Philip; Huber, Robert

2017-07-01

Two-photon-excited fluorescence lifetime imaging microscopy (FLIM) is a chemically specific 3-D sensing modality providing valuable information about the microstructure, composition and function of a sample. However, a more widespread application of this technique is hindered by the need for a sophisticated ultra-short pulse laser source and by speed limitations of current FLIM detection systems. To overcome these limitations, we combined a robust sub-nanosecond fiber laser as the excitation source with high analog bandwidth detection. Due to the long pulse length in our configuration, more fluorescence photons are generated per pulse, which allows us to derive the lifetime with a single excitation pulse only. In this paper, we show high quality FLIM images acquired at a pixel rate of 1 MHz. This approach is a promising candidate for an easy-to-use and benchtop FLIM system to make this technique available to a wider research community.

Towards two-photon excited endogenous fluorescence lifetime imaging microendoscopy

PubMed Central

Hage, C. H.; Leclerc, P.; Brevier, J.; Fabert, M.; Le Nézet, C.; Kudlinski, A.; Héliot, L.; Louradour, F.

2017-01-01

In situ fluorescence lifetime imaging microscopy (FLIM) in an endoscopic configuration of the endogenous biomarker nicotinamide adenine dinucleotide (NADH) has a great potential for malignant tissue diagnosis. Moreover, two-photon nonlinear excitation provides intrinsic optical sectioning along with enhanced imaging depth. We demonstrate, for the first time to our knowledge, nonlinear endogenous FLIM in a fibered microscope with proximal detection, applied to NADH in cultured cells, as a first step to a nonlinear endomicroscope, using a double-clad microstructured fiber with convenient fiber length (> 3 m) and excitation pulse duration (≈50 fs). Fluorescence photons are collected by the fiber inner cladding and we show that its contribution to the impulse response function (IRF), which originates from its intermodal and chromatic dispersions, is small (< 600 ps) and stable for lengths up to 8 m and allows for short lifetime measurements. We use the phasor representation as a quick visualization tool adapted to the endoscopy speed requirements. PMID:29359093

Femtosecond imaging of nonlinear acoustics in gold.

PubMed

Pezeril, Thomas; Klieber, Christoph; Shalagatskyi, Viktor; Vaudel, Gwenaelle; Temnov, Vasily; Schmidt, Oliver G; Makarov, Denys

2014-02-24

We have developed a high-sensitivity, low-noise femtosecond imaging technique based on pump-probe time-resolved measurements with a standard CCD camera. The approach used in the experiment is based on lock-in acquisitions of images generated by a femtosecond laser probe synchronized to modulation of a femtosecond laser pump at the same rate. This technique allows time-resolved imaging of laser-excited phenomena with femtosecond time resolution. We illustrate the technique by time-resolved imaging of the nonlinear reshaping of a laser-excited picosecond acoustic pulse after propagation through a thin gold layer. Image analysis reveals the direct 2D visualization of the nonlinear acoustic propagation of the picosecond acoustic pulse. Many ultrafast pump-probe investigations can profit from this technique because of the wealth of information it provides over a typical single diode and lock-in amplifier setup, for example it can be used to image ultrasonic echoes in biological samples.

Light-induced damage and its diagnosis in two-photon excited autofluorescence imaging of retinal pigment epithelium cells

NASA Astrophysics Data System (ADS)

Chen, Danni; Qu, Junle; Xu, Gaixia; Zhao, Lingling; Niu, Hanben

2007-05-01

In this paper, a novel method for the differentiation of the retinal pigment epithelium (RPE) cells after light-induced damage by two-photon excitation is presented. Fresh samples of RPE cells of pig eyes are obtained from local slaughterhouse. Light-induced damage is produced by the output from Ti: sapphire laser which is focused onto the RPE layer. We study the change of the autofluorescence properties of RPE after two-photon excitation with the same wavelength. Preliminary results show that after two-photon excitation, there are two clear changes in the emission spectrum. The first change is the blue-shift of the emission peak. The emission peak of the intact RPE is located at 592nm, and after excitation, it shifts to 540nm. It is supposed that the excitation has led to the increased autofluorescence of flavin whose emission peak is located at 540nm. The second change is the increased intensity of the emission peak, which might be caused by the accelerated aging because the autofluorescence of RPE would increase during aging process. Experimental results indicate that two-photon excitation could not only lead to the damage of the RPE cells in multiphoton RPE imaging, but also provide an evaluation of the light-induced damage.

Image gathering and coding for digital restoration: Information efficiency and visual quality

NASA Technical Reports Server (NTRS)

Huck, Friedrich O.; John, Sarah; Mccormick, Judith A.; Narayanswamy, Ramkumar

1989-01-01

Image gathering and coding are commonly treated as tasks separate from each other and from the digital processing used to restore and enhance the images. The goal is to develop a method that allows us to assess quantitatively the combined performance of image gathering and coding for the digital restoration of images with high visual quality. Digital restoration is often interactive because visual quality depends on perceptual rather than mathematical considerations, and these considerations vary with the target, the application, and the observer. The approach is based on the theoretical treatment of image gathering as a communication channel (J. Opt. Soc. Am. A2, 1644(1985);5,285(1988). Initial results suggest that the practical upper limit of the information contained in the acquired image data range typically from approximately 2 to 4 binary information units (bifs) per sample, depending on the design of the image-gathering system. The associated information efficiency of the transmitted data (i.e., the ratio of information over data) ranges typically from approximately 0.3 to 0.5 bif per bit without coding to approximately 0.5 to 0.9 bif per bit with lossless predictive compression and Huffman coding. The visual quality that can be attained with interactive image restoration improves perceptibly as the available information increases to approximately 3 bifs per sample. However, the perceptual improvements that can be attained with further increases in information are very subtle and depend on the target and the desired enhancement.

Modelling of radio frequency sheath and fast wave coupling on the realistic ion cyclotron resonant antenna surroundings and the outer wall

NASA Astrophysics Data System (ADS)

Lu, L.; Colas, L.; Jacquot, J.; Després, B.; Heuraux, S.; Faudot, E.; Van Eester, D.; Crombé, K.; Křivská, A.; Noterdaeme, J.-M.; Helou, W.; Hillairet, J.

2018-03-01

In order to model the sheath rectification in a realistic geometry over the size of ion cyclotron resonant heating (ICRH) antennas, the self-consistent sheaths and waves for ICH (SSWICH) code couples self-consistently the RF wave propagation and the DC SOL biasing via nonlinear RF and DC sheath boundary conditions applied at plasma/wall interfaces. A first version of SSWICH had 2D (toroidal and radial) geometry, rectangular walls either normal or parallel to the confinement magnetic field B 0 and only included the evanescent slow wave (SW) excited parasitically by the ICRH antenna. The main wave for plasma heating, the fast wave (FW) plays no role on the sheath excitation in this version. A new version of the code, 2D SSWICH-full wave, was developed based on the COMSOL software, to accommodate full RF field polarization and shaped walls tilted with respect to B 0 . SSWICH-full wave simulations have shown the mode conversion of FW into SW occurring at the sharp corners where the boundary shape varies rapidly. It has also evidenced ‘far-field’ sheath oscillations appearing at the shaped walls with a relatively long magnetic connection length to the antenna, that are only accessible to the propagating FW. Joint simulation, conducted by SSWICH-full wave within a multi-2D approach excited using the 3D wave coupling code (RAPLICASOL), has recovered the double-hump poloidal structure measured in the experimental temperature and potential maps when only the SW is modelled. The FW contribution on the potential poloidal structure seems to be affected by the 3D effects, which was ignored in the current stage. Finally, SSWICH-full wave simulation revealed the left-right asymmetry that has been observed extensively in the unbalanced strap feeding experiments, suggesting that the spatial proximity effects in RF sheath excitation, studied for SW only previously, is still important in the vicinity of the wave launcher under full wave polarizations.

Joint sparse coding based spatial pyramid matching for classification of color medical image.

PubMed

Shi, Jun; Li, Yi; Zhu, Jie; Sun, Haojie; Cai, Yin

2015-04-01

Although color medical images are important in clinical practice, they are usually converted to grayscale for further processing in pattern recognition, resulting in loss of rich color information. The sparse coding based linear spatial pyramid matching (ScSPM) and its variants are popular for grayscale image classification, but cannot extract color information. In this paper, we propose a joint sparse coding based SPM (JScSPM) method for the classification of color medical images. A joint dictionary can represent both the color information in each color channel and the correlation between channels. Consequently, the joint sparse codes calculated from a joint dictionary can carry color information, and therefore this method can easily transform a feature descriptor originally designed for grayscale images to a color descriptor. A color hepatocellular carcinoma histological image dataset was used to evaluate the performance of the proposed JScSPM algorithm. Experimental results show that JScSPM provides significant improvements as compared with the majority voting based ScSPM and the original ScSPM for color medical image classification. Copyright © 2014 Elsevier Ltd. All rights reserved.

Experimental differential cross sections, level densities, and spin cutoffs as a testing ground for nuclear reaction codes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Voinov, Alexander V.; Grimes, Steven M.; Brune, Carl R.

Proton double-differential cross sections from 59Co(α,p) 62Ni, 57Fe(α,p) 60Co, 56Fe( 7Li,p) 62Ni, and 55Mn( 6Li,p) 60Co reactions have been measured with 21-MeV α and 15-MeV lithium beams. Cross sections have been compared against calculations with the empire reaction code. Different input level density models have been tested. It was found that the Gilbert and Cameron [A. Gilbert and A. G. W. Cameron, Can. J. Phys. 43, 1446 (1965)] level density model is best to reproduce experimental data. Level densities and spin cutoff parameters for 62Ni and 60Co above the excitation energy range of discrete levels (in continuum) have been obtainedmore » with a Monte Carlo technique. Furthermore, excitation energy dependencies were found to be inconsistent with the Fermi-gas model.« less

Numerical Tests and Properties of Waves in Radiating Fluids

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, B M; Klein, R I

2009-09-03

We discuss the properties of an analytical solution for waves in radiating fluids, with a view towards its implementation as a quantitative test of radiation hydrodynamics codes. A homogeneous radiating fluid in local thermodynamic equilibrium is periodically driven at the boundary of a one-dimensional domain, and the solution describes the propagation of the waves thus excited. Two modes are excited for a given driving frequency, generally referred to as a radiative acoustic wave and a radiative diffusion wave. While the analytical solution is well known, several features are highlighted here that require care during its numerical implementation. We compare themore » solution in a wide range of parameter space to a numerical integration with a Lagrangian radiation hydrodynamics code. Our most significant observation is that flux-limited diffusion does not preserve causality for waves on a homogeneous background.« less

Navigation domain representation for interactive multiview imaging.

PubMed

Maugey, Thomas; Daribo, Ismael; Cheung, Gene; Frossard, Pascal

2013-09-01

Enabling users to interactively navigate through different viewpoints of a static scene is a new interesting functionality in 3D streaming systems. While it opens exciting perspectives toward rich multimedia applications, it requires the design of novel representations and coding techniques to solve the new challenges imposed by the interactive navigation. In particular, the encoder must prepare a priori a compressed media stream that is flexible enough to enable the free selection of multiview navigation paths by different streaming media clients. Interactivity clearly brings new design constraints: the encoder is unaware of the exact decoding process, while the decoder has to reconstruct information from incomplete subsets of data since the server generally cannot transmit images for all possible viewpoints due to resource constrains. In this paper, we propose a novel multiview data representation that permits us to satisfy bandwidth and storage constraints in an interactive multiview streaming system. In particular, we partition the multiview navigation domain into segments, each of which is described by a reference image (color and depth data) and some auxiliary information. The auxiliary information enables the client to recreate any viewpoint in the navigation segment via view synthesis. The decoder is then able to navigate freely in the segment without further data request to the server; it requests additional data only when it moves to a different segment. We discuss the benefits of this novel representation in interactive navigation systems and further propose a method to optimize the partitioning of the navigation domain into independent segments, under bandwidth and storage constraints. Experimental results confirm the potential of the proposed representation; namely, our system leads to similar compression performance as classical inter-view coding, while it provides the high level of flexibility that is required for interactive streaming. Because of these unique properties, our new framework represents a promising solution for 3D data representation in novel interactive multimedia services.

Intravital Confocal and Two-photon Imaging of Dual-color Cells and Extracellular Matrix Mimics

PubMed Central

Bal, Ufuk; Andresen, Volker; Baggett, Brenda; Utzinger, Urs

2013-01-01

To optimize imaging of cells in three dimensional culture we studied confocal backscattering, Second Harmonic Generation (SHG) and autofluorescence as source of contrast in extracellular matrix (ECM) mimics and evaluated the attenuation as well as bleaching of endogenous cellular fluorescence signals. All common ECM mimics exhibit contrast observable with confocal reflectance microscopy. SHG imaging on collagen I based hydrogels provides high contrast and good optical penetration depth. Agarose is a useful embedding medium because it allows for large optical penetration and exhibits minimal autofluorescence while still providing good reflectance to detect voids in the embedding medium. We labeled breast cancer cells’ outline with DsRed2 and nucleus with eGFP. DsRed2 can be excited with confocal imaging at 568nm, and with two photon excitation (TPE) in the red and longer NIR. eGFP was excited at 488nm for confocal and in the NIR for TPE. While there is small difference in the bleaching rate for eGFP between confocal and TPE we observed significant difference for DsRed2 where bleaching is strongest during TPE in the red wavelengths and smallest during confocal imaging. After a few hundred microns depth in a collagen I hydrogel, TPE fluorescence becomes twice as strong compared to confocal imaging. PMID:23380006

MIMO nonlinear ultrasonic tomography by propagation and backpropagation method.

PubMed

Dong, Chengdong; Jin, Yuanwei

2013-03-01

This paper develops a fast ultrasonic tomographic imaging method in a multiple-input multiple-output (MIMO) configuration using the propagation and backpropagation (PBP) method. By this method, ultrasonic excitation signals from multiple sources are transmitted simultaneously to probe the objects immersed in the medium. The scattering signals are recorded by multiple receivers. Utilizing the nonlinear ultrasonic wave propagation equation and the received time domain scattered signals, the objects are to be reconstructed iteratively in three steps. First, the propagation step calculates the predicted acoustic potential data at the receivers using an initial guess. Second, the difference signal between the predicted value and the measured data is calculated. Third, the backpropagation step computes updated acoustical potential data by backpropagating the difference signal to the same medium computationally. Unlike the conventional PBP method for tomographic imaging where each source takes turns to excite the acoustical field until all the sources are used, the developed MIMO-PBP method achieves faster image reconstruction by utilizing multiple source simultaneous excitation. Furthermore, we develop an orthogonal waveform signaling method using a waveform delay scheme to reduce the impact of speckle patterns in the reconstructed images. By numerical experiments we demonstrate that the proposed MIMO-PBP tomographic imaging method results in faster convergence and achieves superior imaging quality.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pan, Huilin; Yang, Jiayue; Zhang, Dong

The effect of antisymmetric C–H stretching excitation of CH{sub 4} on the dynamics and reactivity of the O({sup 1}D) + CH{sub 4} → OH + CD{sub 3} reaction at the collision energy of 6.10 kcal/mol has been investigated using the crossed-beam and time-sliced velocity map imaging techniques. The antisymmetric C–H stretching mode excited CH{sub 4} molecule was prepared by direct infrared excitation. From the measured images of the CH{sub 3} products with the infrared laser on and off, the product translational energy and angular distributions were derived for both the ground and vibrationally excited reactions. Experimental results show that themore » vibrational energy of the antisymmetric stretching excited CH{sub 4} reagent is channeled exclusively into the vibrational energy of the OH co-products and, hence, the OH products from the excited-state reaction are about one vibrational quantum hotter than those from the ground-state reaction, and the product angular distributions are barely affected by the vibrational excitation of the CH{sub 4} reagent. The reactivity was found to be suppressed by the antisymmetric stretching excitation of CH{sub 4} for all observed CH{sub 3} vibrational states. The degree of suppression is different for different CH{sub 3} vibrational states: the suppression is about 40%–60% for the ground state and the umbrella mode excited CH{sub 3} products, while for the CH{sub 3} products with one quantum symmetric stretching mode excitation, the suppression is much less pronounced. In consequence, the vibrational state distribution of the CH{sub 3} product from the excited-state reaction is considerably different from that of the ground-state reaction.« less

Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons.

PubMed Central

Fan, G Y; Fujisaki, H; Miyawaki, A; Tsay, R K; Tsien, R Y; Ellisman, M H

1999-01-01

A video-rate (30 frames/s) scanning two-photon excitation microscope has been successfully tested. The microscope, based on a Nikon RCM 8000, incorporates a femtosecond pulsed laser with wavelength tunable from 690 to 1050 nm, prechirper optics for laser pulse-width compression, resonant galvanometer for video-rate point scanning, and a pair of nonconfocal detectors for fast emission ratioing. An increase in fluorescent emission of 1.75-fold is consistently obtained with the use of the prechirper optics. The nonconfocal detectors provide another 2.25-fold increase in detection efficiency. Ratio imaging and optical sectioning can therefore be performed more efficiently without confocal optics. Faster frame rates, at 60, 120, and 240 frames/s, can be achieved with proportionally reduced scan lines per frame. Useful two-photon images can be acquired at video rate with a laser power as low as 2.7 mW at specimen with the genetically modified green fluorescent proteins. Preliminary results obtained using this system confirm that the yellow "cameleons" exhibit similar optical properties as under one-photon excitation conditions. Dynamic two-photon images of cardiac myocytes and ratio images of yellow cameleon-2.1, -3.1, and -3.1nu are also presented. PMID:10233058

Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging

DOE PAGES

Newman, Justin A.; Zhang, Shijie; Sullivan, Shane Z.; ...

2016-05-16

Synchronous digitization, in which an optical sensor is probed synchronously with the firing of an ultrafast laser, was integrated into an optical imaging station for macromolecular crystal positioning prior to synchrotron X-ray diffraction. Using the synchronous digitization instrument, second-harmonic generation, two-photon-excited fluorescence and bright field by laser transmittance were all acquired simultaneously with perfect image registry at up to video-rate (15 frames s –1). A simple change in the incident wavelength enabled simultaneous imaging by two-photon-excited ultraviolet fluorescence, one-photon-excited visible fluorescence and laser transmittance. Development of an analytical model for the signal-to-noise enhancement afforded by synchronous digitization suggests a 15.6-foldmore » improvement over previous photon-counting techniques. This improvement in turn allowed acquisition on nearly an order of magnitude more pixels than the preceding generation of instrumentation and reductions of well over an order of magnitude in image acquisition times. These improvements have allowed detection of protein crystals on the order of 1 µm in thickness under cryogenic conditions in the beamline. Lastly, these capabilities are well suited to support serial crystallography of crystals approaching 1 µm or less in dimension.« less

Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging

DOE Office of Scientific and Technical Information (OSTI.GOV)

Newman, Justin A.; Zhang, Shijie; Sullivan, Shane Z.

Synchronous digitization, in which an optical sensor is probed synchronously with the firing of an ultrafast laser, was integrated into an optical imaging station for macromolecular crystal positioning prior to synchrotron X-ray diffraction. Using the synchronous digitization instrument, second-harmonic generation, two-photon-excited fluorescence and bright field by laser transmittance were all acquired simultaneously with perfect image registry at up to video-rate (15 frames s –1). A simple change in the incident wavelength enabled simultaneous imaging by two-photon-excited ultraviolet fluorescence, one-photon-excited visible fluorescence and laser transmittance. Development of an analytical model for the signal-to-noise enhancement afforded by synchronous digitization suggests a 15.6-foldmore » improvement over previous photon-counting techniques. This improvement in turn allowed acquisition on nearly an order of magnitude more pixels than the preceding generation of instrumentation and reductions of well over an order of magnitude in image acquisition times. These improvements have allowed detection of protein crystals on the order of 1 µm in thickness under cryogenic conditions in the beamline. Lastly, these capabilities are well suited to support serial crystallography of crystals approaching 1 µm or less in dimension.« less

Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging

PubMed Central

Newman, Justin A.; Zhang, Shijie; Sullivan, Shane Z.; Dow, Ximeng Y.; Becker, Michael; Sheedlo, Michael J.; Stepanov, Sergey; Carlsen, Mark S.; Everly, R. Michael; Das, Chittaranjan; Fischetti, Robert F.; Simpson, Garth J.

2016-01-01

Synchronous digitization, in which an optical sensor is probed synchronously with the firing of an ultrafast laser, was integrated into an optical imaging station for macromolecular crystal positioning prior to synchrotron X-ray diffraction. Using the synchronous digitization instrument, second-harmonic generation, two-photon-excited fluorescence and bright field by laser transmittance were all acquired simultaneously with perfect image registry at up to video-rate (15 frames s−1). A simple change in the incident wavelength enabled simultaneous imaging by two-photon-excited ultraviolet fluorescence, one-photon-excited visible fluorescence and laser transmittance. Development of an analytical model for the signal-to-noise enhancement afforded by synchronous digitization suggests a 15.6-fold improvement over previous photon-counting techniques. This improvement in turn allowed acquisition on nearly an order of magnitude more pixels than the preceding generation of instrumentation and reductions of well over an order of magnitude in image acquisition times. These improvements have allowed detection of protein crystals on the order of 1 µm in thickness under cryogenic conditions in the beamline. These capabilities are well suited to support serial crystallography of crystals approaching 1 µm or less in dimension. PMID:27359145

Medical imaging systems

DOEpatents

Frangioni, John V [Wayland, MA

2012-07-24

A medical imaging system provides simultaneous rendering of visible light and fluorescent images. The system may employ dyes in a small-molecule form that remains in a subject's blood stream for several minutes, allowing real-time imaging of the subject's circulatory system superimposed upon a conventional, visible light image of the subject. The system may also employ dyes or other fluorescent substances associated with antibodies, antibody fragments, or ligands that accumulate within a region of diagnostic significance. In one embodiment, the system provides an excitation light source to excite the fluorescent substance and a visible light source for general illumination within the same optical guide that is used to capture images. In another embodiment, the system is configured for use in open surgical procedures by providing an operating area that is closed to ambient light. More broadly, the systems described herein may be used in imaging applications where a visible light image may be usefully supplemented by an image formed from fluorescent emissions from a fluorescent substance that marks areas of functional interest.

Contact resonance atomic force microscopy imaging in air and water using photothermal excitation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kocun, Marta; Labuda, Aleksander; Gannepalli, Anil

2015-08-15

Contact Resonance Force Microscopy (CR-FM) is a leading atomic force microscopy technique for measuring viscoelastic nano-mechanical properties. Conventional piezo-excited CR-FM measurements have been limited to imaging in air, since the “forest of peaks” frequency response associated with acoustic excitation methods effectively masks the true cantilever resonance. Using photothermal excitation results in clean contact, resonance spectra that closely match the ideal frequency response of the cantilever, allowing unambiguous and simple resonance frequency and quality factor measurements in air and liquids alike. This extends the capabilities of CR-FM to biologically relevant and other soft samples in liquid environments. We demonstrate CR-FM inmore » air and water on both stiff silicon/titanium samples and softer polystyrene-polyethylene-polypropylene polymer samples with the quantitative moduli having very good agreement between expected and measured values.« less

Optimization and performance evaluation of a conical mirror based fluorescence molecular tomography imaging system

NASA Astrophysics Data System (ADS)

Zhao, Yue; Zhang, Wei; Zhu, Dianwen; Li, Changqing

2016-03-01

We performed numerical simulations and phantom experiments with a conical mirror based fluorescence molecular tomography (FMT) imaging system to optimize its performance. With phantom experiments, we have compared three measurement modes in FMT: the whole surface measurement mode, the transmission mode, and the reflection mode. Our results indicated that the whole surface measurement mode performed the best. Then, we applied two different neutral density (ND) filters to improve the measurement's dynamic range. The benefits from ND filters are not as much as predicted. Finally, with numerical simulations, we have compared two laser excitation patterns: line and point. With the same excitation position number, we found that the line laser excitation had slightly better FMT reconstruction results than the point laser excitation. In the future, we will implement Monte Carlo ray tracing simulations to calculate multiple reflection photons, and create a look-up table accordingly for calibration.

Novel approach to multispectral image compression on the Internet

NASA Astrophysics Data System (ADS)

Zhu, Yanqiu; Jin, Jesse S.

2000-10-01

Still image coding techniques such as JPEG have been always applied onto intra-plane images. Coding fidelity is always utilized in measuring the performance of intra-plane coding methods. In many imaging applications, it is more and more necessary to deal with multi-spectral images, such as the color images. In this paper, a novel approach to multi-spectral image compression is proposed by using transformations among planes for further compression of spectral planes. Moreover, a mechanism of introducing human visual system to the transformation is provided for exploiting the psycho visual redundancy. The new technique for multi-spectral image compression, which is designed to be compatible with the JPEG standard, is demonstrated on extracting correlation among planes based on human visual system. A high measure of compactness in the data representation and compression can be seen with the power of the scheme taken into account.

Optical noise-free image encryption based on quick response code and high dimension chaotic system in gyrator transform domain

NASA Astrophysics Data System (ADS)

Sui, Liansheng; Xu, Minjie; Tian, Ailing

2017-04-01

A novel optical image encryption scheme is proposed based on quick response code and high dimension chaotic system, where only the intensity distribution of encoded information is recorded as ciphertext. Initially, the quick response code is engendered from the plain image and placed in the input plane of the double random phase encoding architecture. Then, the code is encrypted to the ciphertext with noise-like distribution by using two cascaded gyrator transforms. In the process of encryption, the parameters such as rotation angles and random phase masks are generated as interim variables and functions based on Chen system. A new phase retrieval algorithm is designed to reconstruct the initial quick response code in the process of decryption, in which a priori information such as three position detection patterns is used as the support constraint. The original image can be obtained without any energy loss by scanning the decrypted code with mobile devices. The ciphertext image is the real-valued function which is more convenient for storing and transmitting. Meanwhile, the security of the proposed scheme is enhanced greatly due to high sensitivity of initial values of Chen system. Extensive cryptanalysis and simulation have performed to demonstrate the feasibility and effectiveness of the proposed scheme.

Evaluating the Feasibility of Acoustic Radiation Force Impulse Shear Wave Elasticity Imaging of the Uterine Cervix With an Intracavity Array: A Simulation Study

PubMed Central

Feltovich, Helen; Homyk, Andrew D.; Carlson, Lindsey C.; Hall, Timothy J.

2015-01-01

The uterine cervix softens, shortens, and dilates throughout pregnancy in response to progressive disorganization of its layered collagen microstructure. This process is an essential part of normal pregnancy, but premature changes are associated with preterm birth. Clinically, there are no reliable noninvasive methods to objectively measure cervical softening or assess cervical microstructure. The goal of these preliminary studies was to evaluate the feasibility of using an intracavity ultrasound array to generate acoustic radiation force impulse (ARFI) excitations in the uterine cervix through simulation, and to optimize the acoustic radiation force (ARF) excitation for shear wave elasticity imaging (SWEI) of the tissue stiffness. The cervix is a unique soft tissue target for SWEI because it has significantly greater acoustic attenuation (α = 1.3 to 2.0 dB·cm−1·MHz−1) than other soft tissues, and the pathology being studied tends to lead to an increase in tissue compliance, with healthy cervix being relatively stiff compared with other soft tissues (E ≈ 25 kPa). Additionally, the cervix can only be accessed in vivo using a transvaginal or catheter-based array, which places additional constraints on the excitation focal characteristics that can be used during SWEI. Finite element method (FEM) models of SWEI show that larger-aperture, catheter-based arrays can utilize excitation frequencies up to 7 MHz to generate adequate focal gain up to focal depths 10 to 15 mm deep, with higher frequencies suffering from excessive amounts of near-field acoustic attenuation. Using full-aperture excitations can yield ~40% increases in ARFI-induced displacements, but also restricts the depth of field of the excitation to ~0.5 mm, compared with 2 to 6 mm, which limits the range that can be used for shear wave characterization of the tissue. The center-frequency content of the shear wave particle velocity profiles ranges from 1.5 to 2.5 kHz, depending on the focal configuration and the stiffness of the material being imaged. Overall, SWEI is possible using catheter-based imaging arrays to generate adequate displacements in cervical tissue for shear wave imaging, although specific considerations must be made when optimizing these arrays for this shear wave imaging application. PMID:24081254

Value of epicardial potential maps in localizing pre-excitation sites for radiofrequency ablation. A simulation study

NASA Astrophysics Data System (ADS)

Hren, Rok

1998-06-01

Using computer simulations, we systematically investigated the limitations of an inverse solution that employs the potential distribution on the epicardial surface as an equivalent source model in localizing pre-excitation sites in Wolff-Parkinson-White syndrome. A model of the human ventricular myocardium that features an anatomically accurate geometry, an intramural rotating anisotropy and a computational implementation of the excitation process based on electrotonic interactions among cells, was used to simulate body surface potential maps (BSPMs) for 35 pre-excitation sites positioned along the atrioventricular ring. Two individualized torso models were used to account for variations in torso boundaries. Epicardial potential maps (EPMs) were computed using the L-curve inverse solution. The measure for accuracy of the localization was the distance between a position of the minimum in the inverse EPMs and the actual site of pre-excitation in the ventricular model. When the volume conductor properties and lead positions of the torso were precisely known and the measurement noise was added to the simulated BSPMs, the minimum in the inverse EPMs was at 12 ms after the onset on average within cm of the pre-excitation site. When the standard torso model was used to localize the sites of onset of the pre-excitation sequence initiated in individualized male and female torso models, the mean distance between the minimum and the pre-excitation site was cm for the male torso and cm for the female torso. The findings of our study indicate that a location of the minimum in EPMs computed using the inverse solution can offer non-invasive means for pre-interventional planning of the ablative treatment.

Advanced multiphoton methods for in vitro and in vivo functional imaging of mouse retinal neurons (Conference Presentation)

NASA Astrophysics Data System (ADS)

Cohen, Noam; Schejter, Adi; Farah, Nairouz; Shoham, Shy

2016-03-01

Studying the responses of retinal ganglion cell (RGC) populations has major significance in vision research. Multiphoton imaging of optogenetic probes has recently become the leading approach for visualizing neural populations and has specific advantages for imaging retinal activity during visual stimulation, because it leads to reduced direct photoreceptor excitation. However, multiphoton retinal activity imaging is not straightforward: point-by-point scanning leads to repeated neural excitation while optical access through the rodent eye in vivo has proven highly challenging. Here, we present two enabling optical designs for multiphoton imaging of responses to visual stimuli in mouse retinas expressing calcium indicators. First, we present an imaging solution based on Scanning Line Temporal Focusing (SLITE) for rapidly imaging neuronal activity in vitro. In this design, we scan a temporally focused line rather than a point, increasing the scan speed and reducing the impact of repeated excitation, while maintaining high optical sectioning. Second, we present the first in vivo demonstration of two-photon imaging of RGC activity in the mouse retina. To obtain these cellular resolution recordings we integrated an illumination path into a correction-free imaging system designed using an optical model of the mouse eye. This system can image at multiple depths using an electronically tunable lens integrated into its optical path. The new optical designs presented here overcome a number of outstanding obstacles, allowing the study of rapid calcium- and potentially even voltage-indicator signals both in vitro and in vivo, thereby bringing us a step closer toward distributed monitoring of action potentials.

Evidence for concerted ring opening and C-Br bond breaking in UV-excited bromocyclopropane.

PubMed

Pandit, Shubhrangshu; Preston, Thomas J; King, Simon J; Vallance, Claire; Orr-Ewing, Andrew J

2016-06-28

Photodissociation of gaseous bromocyclopropane via its A-band continuum has been studied at excitation wavelengths ranging from 230 nm to 267 nm. Velocity-map images of ground-state bromine atoms (Br), spin-orbit excited bromine atoms (Br(∗)), and C3H5 hydrocarbon radicals reveal the kinetic energies of these various photofragments. Both Br and Br(∗) atoms are predominantly generated via repulsive excited electronic states in a prompt photodissociation process in which the hydrocarbon co-fragment is a cyclopropyl radical. However, the images obtained at the mass of the hydrocarbon radical fragment identify a channel with total kinetic energy greater than that deduced from the Br and Br(∗) images, and with a kinetic energy distribution that exceeds the energetic limit for Br + cyclopropyl radical products. The velocity-map images of these C3H5 fragments have lower angular anisotropies than measured for Br and Br(∗), indicating molecular restructuring during dissociation. The high kinetic energy C3H5 signals are assigned to allyl radicals generated by a minor photochemical pathway which involves concerted C-Br bond dissociation and cyclopropyl ring-opening following single ultraviolet (UV)-photon absorption. Slow photofragments also contribute to the velocity map images obtained at the C3H5 radical mass, but the corresponding slow Br atoms are not observed. These features in the images are attributed to C3H5 (+) from the photodissociation of the C3H5Br(+) molecular cation following two-photon ionization of the parent compound. This assignment is confirmed by 118-nm vacuum ultraviolet ionization studies that prepare the molecular cation in its ground electronic state prior to UV photodissociation.

Efficient burst image compression using H.265/HEVC

NASA Astrophysics Data System (ADS)

Roodaki-Lavasani, Hoda; Lainema, Jani

2014-02-01

New imaging use cases are emerging as more powerful camera hardware is entering consumer markets. One family of such use cases is based on capturing multiple pictures instead of just one when taking a photograph. That kind of a camera operation allows e.g. selecting the most successful shot from a sequence of images, showing what happened right before or after the shot was taken or combining the shots by computational means to improve either visible characteristics of the picture (such as dynamic range or focus) or the artistic aspects of the photo (e.g. by superimposing pictures on top of each other). Considering that photographic images are typically of high resolution and quality and the fact that these kind of image bursts can consist of at least tens of individual pictures, an efficient compression algorithm is desired. However, traditional video coding approaches fail to provide the random access properties these use cases require to achieve near-instantaneous access to the pictures in the coded sequence. That feature is critical to allow users to browse the pictures in an arbitrary order or imaging algorithms to extract desired pictures from the sequence quickly. This paper proposes coding structures that provide such random access properties while achieving coding efficiency superior to existing image coders. The results indicate that using HEVC video codec with a single reference picture fixed for the whole sequence can achieve nearly as good compression as traditional IPPP coding structures. It is also shown that the selection of the reference frame can further improve the coding efficiency.

The AAPM/RSNA physics tutorial for residents. Basic physics of MR imaging: an introduction.

PubMed

Hendrick, R E

1994-07-01

This article provides an introduction to the basic physical principles of magnetic resonance (MR) imaging. Essential basic concepts such as nuclear magnetism, tissue magnetization, precession, excitation, and tissue relaxation properties are presented. Hydrogen spin density and tissue relaxation times T1, T2, and T2* are explained. The basic elements of a planar MR pulse sequence are described: section selection during tissue excitation, phase encoding, and frequency encoding during signal measurement.

Multiphoton spectroscopy of human skin in vivo

NASA Astrophysics Data System (ADS)

Breunig, Hans G.; Weinigel, Martin; König, Karsten

2012-03-01

In vivo multiphoton-intensity images and emission spectra of human skin are reported. Optical sections from different depths of the epidermis and dermis have been measured with near-infrared laser-pulse excitation. While the intensity images reveal information on the morphology, the spectra show emission characteristics of main endogenous skin fluorophores like keratin, NAD(P)H, melanin, elastin and collagen as well as of second harmonic generation induced by the excitation-light interaction with the dermal collagen network.

High-resolution imaging gamma-ray spectroscopy with externally segmented germanium detectors

NASA Technical Reports Server (NTRS)

Callas, J. L.; Mahoney, W. A.; Varnell, L. S.; Wheaton, W. A.

1993-01-01

Externally segmented germanium detectors promise a breakthrough in gamma-ray imaging capabilities while retaining the superb energy resolution of germanium spectrometers. An angular resolution of 0.2 deg becomes practical by combining position-sensitive germanium detectors having a segment thickness of a few millimeters with a one-dimensional coded aperture located about a meter from the detectors. Correspondingly higher angular resolutions are possible with larger separations between the detectors and the coded aperture. Two-dimensional images can be obtained by rotating the instrument. Although the basic concept is similar to optical or X-ray coded-aperture imaging techniques, several complicating effects arise because of the penetrating nature of gamma rays. The complications include partial transmission through the coded aperture elements, Compton scattering in the germanium detectors, and high background count rates. Extensive electron-photon Monte Carlo modeling of a realistic detector/coded-aperture/collimator system has been performed. Results show that these complicating effects can be characterized and accounted for with no significant loss in instrument sensitivity.

Absorption characterization of immersion medium for multiphoton microscopy at the 1700nm window

NASA Astrophysics Data System (ADS)

Wen, Wenhui; Qiu, Ping

2017-02-01

Larger imaging depth is the quest of almost all the imaging modalities, including multiphoton microscopy (MPM). Recently, it has been domonstrated that excitation at the 1700-nm helps extending imaging depth in MPM, optical coherence tomography, as well as photoacoustic imaging compared with excitation at other wavelengths. In MPM, immersion objective lenses with high numerical aperture (NA) are typically used to achieve better signal resolution, higer signal collection efficiency, and stronger signal generation. Although physically short ( mm), this extra optical path length traversed by the excitation light inevitably introduces absorption of the excitation light, and as a result leads to a decrease in the signal generation. Here we demonstrate experimental characterization of absorption spectrum of various immersion media at the 1700-nm window, including water (H2O), deuterium oxide (D2O), and several brands of immersion oil. Our results identify either the best immersion medium for a specific wavelength, or the best wavelength for a specific immersion medium at the 1700-nm window. Furthermore, through quantitative MPM experiments comparing different immersion media, we show that the MPM signal levels can be enhanced by more than ten fold simply by selecting the proper immersion medium, in good agreement with theoretical expectation based on the absorption measurement. Our results will offer guidelines for signal optimization in MPM at the 1700-nm window.

Two-photon excited fluorescence microscopy application for ex vivo investigation of ocular fundus samples

NASA Astrophysics Data System (ADS)

Peters, Sven; Hammer, Martin; Schweitzer, Dietrich

2011-07-01

Two-photon excited fluorescence (TPEF) imaging of ocular tissue has recently become a promising tool in ophthalmology for diagnostic and research purposes. The feasibility and the advantages of TPEF imaging, namely deeper tissue penetration and improved high-resolution imaging of microstructures, have been demonstrated lately using human ocular samples. The autofluorescence properties of endogenous fluorophores in ocular fundus tissue are well known from spectrophotometric analysis. But fluorophores, especially when it comes to fluorescence lifetime, typically display a dependence of their fluorescence properties on local environmental parameters. Hence, a more detailed investigation of ocular fundus autofluorescence ideally in vivo is of utmost interest. The aim of this study is to determine space-resolved the stationary and time-resolved fluorescence properties of endogenous fluorophores in ex vivo porcine ocular fundus samples by means of two-photon excited fluorescence spectrum and lifetime imaging microscopy (FSIM/FLIM). By our first results, we characterized the autofluorescence of individual anatomical structures of porcine retina samples excited at 760 nm. The fluorescence properties of almost all investigated retinal layers are relatively homogenous. But as previously unknown, ganglion cell bodies show a significantly shorter fluorescence lifetime compared to the adjacent mueller cells. Since all retinal layers exhibit bi-exponential autofluorescence decays, we were able to achieve a more precise characterization of fluorescence properties of endogenous fluorophores compared to a present in vivo FLIM approach by confocal scanning laser ophthalmoscope (cSLO).