Cloning and sequence analysis of the meso-diaminopimelate decarboxylase gene from Bacillus methanolicus MGA3 and comparison to other decarboxylase genes.

PubMed

Mills, D A; Flickinger, M C

1993-09-01

The lysA gene of Bacillus methanolicus MGA3 was cloned by complementation of an auxotrophic Escherichia coli lysA22 mutant with a genomic library of B. methanolicus MGA3 chromosomal DNA. Subcloning localized the B. methanolicus MGA3 lysA gene into a 2.3-kb SmaI-SstI fragment. Sequence analysis of the 2.3-kb fragment indicated an open reading frame encoding a protein of 48,223 Da, which was similar to the meso-diaminopimelate (DAP) decarboxylase amino acid sequences of Bacillus subtilis (62%) and Corynebacterium glutamicum (40%). Amino acid sequence analysis indicated several regions of conservation among bacterial DAP decarboxylases, eukaryotic ornithine decarboxylases, and arginine decarboxylases, suggesting a common structural arrangement for positioning of substrate and the cofactor pyridoxal 5'-phosphate. The B. methanolicus MGA3 DAP decarboxylase was shown to be a dimer (M(r) 86,000) with a subunit molecular mass of approximately 50,000 Da. This decarboxylase is inhibited by lysine (Ki = 0.93 mM) with a Km of 0.8 mM for DAP. The inhibition pattern suggests that the activity of this enzyme in lysine-overproducing strains of B. methanolicus MGA3 may limit lysine synthesis.

Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast*

PubMed Central

Burr, Risa; Stewart, Emerson V.; Shao, Wei; Zhao, Shan; Hannibal-Bach, Hans Kristian; Ejsing, Christer S.; Espenshade, Peter J.

2016-01-01

Eukaryotic lipid synthesis is oxygen-dependent with cholesterol synthesis requiring 11 oxygen molecules and fatty acid desaturation requiring 1 oxygen molecule per double bond. Accordingly, organisms evaluate oxygen availability to control lipid homeostasis. The sterol regulatory element-binding protein (SREBP) transcription factors regulate lipid homeostasis. In mammals, SREBP-2 controls cholesterol biosynthesis, whereas SREBP-1 controls triacylglycerol and glycerophospholipid biosynthesis. In the fission yeast Schizosaccharomyces pombe, the SREBP-2 homolog Sre1 regulates sterol homeostasis in response to changing sterol and oxygen levels. However, notably missing is an SREBP-1 analog that regulates triacylglycerol and glycerophospholipid homeostasis in response to low oxygen. Consistent with this, studies have shown that the Sre1 transcription factor regulates only a fraction of all genes up-regulated under low oxygen. To identify new regulators of low oxygen adaptation, we screened the S. pombe nonessential haploid deletion collection and identified 27 gene deletions sensitive to both low oxygen and cobalt chloride, a hypoxia mimetic. One of these genes, mga2, is a putative transcriptional activator. In the absence of mga2, fission yeast exhibited growth defects under both normoxia and low oxygen conditions. Mga2 transcriptional targets were enriched for lipid metabolism genes, and mga2Δ cells showed disrupted triacylglycerol and glycerophospholipid homeostasis, most notably with an increase in fatty acid saturation. Indeed, addition of exogenous oleic acid to mga2Δ cells rescued the observed growth defects. Together, these results establish Mga2 as a transcriptional regulator of triacylglycerol and glycerophospholipid homeostasis in S. pombe, analogous to mammalian SREBP-1. PMID:27053105

6-Phosphofructokinase and ribulose-5-phosphate 3-epimerase in methylotrophic Bacillus methanolicus ribulose monophosphate cycle.

PubMed

Le, Simone Balzer; Heggeset, Tonje Marita Bjerkan; Haugen, Tone; Nærdal, Ingemar; Brautaset, Trygve

2017-05-01

D-Ribulose-5-phosphate-3-epimerase (RPE) and 6-phosphofructokinase (PFK) catalyse two reactions in the ribulose monophosphate (RuMP) cycle in Bacillus methanolicus. The B. methanolicus wild-type strain MGA3 possesses two putative rpe and pfk genes encoded on plasmid pBM19 (rpe1-MGA3 and pfk1-MGA3) and on the chromosome (rpe2-MGA3 and pfk2-MGA3). The wild-type strain PB1 also encodes putative rpe and pfk genes on plasmid pBM20 (rpe1-PB1 and pfk1-PB1*); however, it only harbours a chromosomal pfk gene (pfk2-PB1). Transcription of the plasmid-encoded genes was 10-fold to 15-fold upregulated in cells growing on methanol compared to mannitol, while the chromosomal genes were transcribed at similar levels under both conditions in both strains. All seven gene products were recombinantly produced in Escherichia coli, purified and biochemically characterized. All three RPEs were active as hexamers, catalytically stimulated by Mg 2+ and Mn 2+ and displayed similar K' values (56-75 μM) for ribulose 5-phosphate. Rpe2-MGA3 showed displayed 2-fold lower V max (49 U/mg) and a significantly reduced thermostability compared to the two Rpe1 proteins. Pfk1-PB1* was shown to be non-functional. The PFKs were active both as octamers and as tetramers, were catalytically stimulated by Mg 2+ and Mn 2+ , and displayed similar thermostabilities. The PFKs have similar K m values for fructose 6-phosphate (0.61-0.94 μM) and for ATP (0.38-0.82 μM), while Pfk1-MGA3 had a 2-fold lower V max (6.3 U/mg) compared to the two Pfk2 proteins. Our results demonstrate that MGA3 and PB1 exert alternative solutions to plasmid-dependent methylotrophy, including genetic organization, regulation, and biochemistry of RuMP cycle enzymes.

Function and dynamics of aptamers: A case study on the malachite green aptamer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Tianjiao

Aptamers are short single-stranded nucleic acids that can bind to their targets with high specificity and high affinity. To study aptamer function and dynamics, the malachite green aptamer was chosen as a model. Malachite green (MG) bleaching, in which an OH- attacks the central carbon (C1) of MG, was inhibited in the presence of the malachite green aptamer (MGA). The inhibition of MG bleaching by MGA could be reversed by an antisense oligonucleotide (AS) complementary to the MGA binding pocket. Computational cavity analysis of the NMR structure of the MGA-MG complex predicted that the OH - is sterically excluded frommore » the C1 of MG. The prediction was confirmed experimentally using variants of the MGA with changes in the MG binding pocket. This work shows that molecular reactivity can be reversibly regulated by an aptamer-AS pair based on steric hindrance. In addition to demonstrate that aptamers could control molecular reactivity, aptamer dynamics was studied with a strategy combining molecular dynamics (MD) simulation and experimental verification. MD simulation predicted that the MG binding pocket of the MGA is largely pre-organized and that binding of MG involves reorganization of the pocket and a simultaneous twisting of the MGA terminal stems around the pocket. MD simulation also provided a 3D-structure model of unoccupied MGA that has not yet been obtained by biophysical measurements. These predictions were consistent with biochemical and biophysical measurements of the MGA-MG interaction including RNase I footprinting, melting curves, thermodynamic and kinetic constants measurement. This work shows that MD simulation can be used to extend our understanding of the dynamics of aptamer-target interaction which is not evident from static 3D-structures. To conclude, I have developed a novel concept to control molecular reactivity by an aptamer based on steric protection and a strategy to study the dynamics of aptamer-target interaction by combining MD simulation and experimental verification. The former has potential application in controlling metabolic reactions and protein modifications by small reactants and the latter may serve as a general approach to study the dynamics of aptamer-target interaction for new insights into mechanisms of aptamer-target recognition.« less

Effect of dietary melengestrol acetate on the incidence of acute interstitial pneumonia in feedlot heifers

PubMed Central

McAllister, Tim A.; Ayroud, Mejid; Bray, Tammy M.; Yost, Garold S.

2006-01-01

Abstract Over a 3-y period, 906 000 cattle were monitored in 23 feedlots in southern Alberta for symptoms of acute interstitial pneumonia (AIP). Plasma, urine, and lung tissue were collected at slaughter from 299 animals clinically diagnosed with AIP and from 156 healthy penmates and analyzed for 3-methylindole (3MI) derivatives and reduced glutathione concentration. From each animal, the left lung was subsampled for histologic examination. Concentrations of glutathione in lung tissue were reduced (P < 0.001) in animals showing clinical symptoms of AIP as compared with their asymptomatic penmates. Animals histologically confirmed as having AIP had higher levels of 3MI protein adducts in blood and lung tissue (P < 0.05) than did emergency-slaughtered animals without AIP. Within feedlots, where pens of heifers were fed either a standard dosage of melengestrol acetate (MGA) or none, the rate of death attributable to AIP was similar between treatment groups, but emergency slaughter after clinical diagnosis of AIP was done 3.2 times more often (P < 0.001) in the MGA-fed heifers than in the group not fed MGA. Use of MGA did not influence glutathione concentration. As growth performance of heifers given steroidal implants may not be improved by feeding MGA, the most cost-effective method of reducing the incidence of AIP-related emergency slaughter in feedlot heifers may be to eliminate MGA from the diet. PMID:16850945

FRAM telescope - monitoring of atmospheric extinction and variable star photometry

NASA Astrophysics Data System (ADS)

Jurysek, J.; Honkova, K.; Masek, M.

2015-02-01

The FRAM (F/(Ph)otometric Robotic Atmospheric Monitor) telescope is a part of the Pierre Auger Observatory (PAO) located near town Malargüe in Argentina. The main task of the FRAM telescope is the continuous night - time monitoring of the atmospheric extinction and its wavelength dependence. The current methodology of the measurement of a atmospheric extinction and for instrumentation properties also allows simultaneous observation of other interesting astronomical targets. The current observations of the FRAM telescope are focused on the photometry of eclipsing binaries, positional refinement of minor bodies of the Solar system and observations of optical counterparts of gamma ray bursts. In this contribution, we briefly describe the main purpose of the FRAM telescope for the PAO and we also present its current astrono mical observing program.

Suppression of ovulation in Nile Hippopotamus (Hippopotamus amphibious) using melengestrol acetate-treated feed or high dose Depo-Provera injection.

PubMed

Wheaton, Catharine J; Joseph, Sharon; Reid, Kelly; Webster, Tricia; Richards, Mary; Forde, Holly M; Savage, Anne

2007-07-01

Analysis of fecal progestogen profiles during Depo-Provera injection (1,200 mg; DEPO, Pfizer Inc., New York, NY), melengestrol acetate (MGA) in feed (2 or 3 mg/head/day), and a combination treatment (DEPO+MGA) are presented for nine captive female Nile hippos housed at Disney's Animal Kingdom in Florida. All tested treatments reduced fecal progestogen elevations successfully to durations consistent with prevention of ovulation for a portion of the treatment period. Percentage of treatment months with suppression of luteal phases indicative of ovulation was maximal for high-dose MGA (91.7+/-13.9%) and DEPO+MGA (91.7+/-20.4%), followed by DEPO injection alone (69.2+/-13.9%) and low-dose MGA (57.6+/-33.2%). Both 1,200 mg DEPO and low-dose MGA (2.0 mg/day) treatments were insufficient to prevent an apparent seasonal breakthrough of ovarian activity from June-August 2002. Although luteal phases were observed, no females conceived during those months. Overall, in 133.5 treatment months with females housed with an adult male, one female conceived during the transition period between treatments. After cessation of contraceptive treatment, average latency to first normal ovarian cycle was 80.6+/-19.5 days (range = 22-179 days). Up to 12 months post-treatment, however, successive cycles were often irregular with evidence of short periods of anovulation and shortened luteal phases in all females monitored. In conclusion, high dose and combination treatments were most successful in preventing progestogen increases indicative of ovulation in hippos. Zoo Biol 26:259-274, 2007. (c) 2007 Wiley-Liss, Inc.

Facile Preparation of Magnetic Graphene Oxide and Attapulgite Composite Adsorbent for the Adsorption of Ni (II)

NASA Astrophysics Data System (ADS)

Bigui, Wei; Xiaofei, Zhu; Xiabing, Cheng

2017-12-01

Graphene oxide (GO) is an excellent absorbent for heavy ion from wastewater, but it is hard to separate from water. To improve the adsorption capacity and separation performance of GO to nickel-containing wastewater, a composite magnetic GO-ATP adsorbent (MGA) was prepared by magnetizing GO and attapulgite (ATP) using ferroferric oxide and then carrying out hydrothermal reaction. The adsorption capacity and mechanism of MGA were investigated based on Ni2+ as targeted pollutant. Experimental results showed that the pH value significantly affects the removal rate of Ni2+, which is mainly due to that OH- in wastewater reacts with Ni2+, resulting in sediment that leads to the increase of removal rate. MGA can achieve max adsorption capacity of Ni2+ to 190.8 mg/g at pH = 5, and the adsorption process was mainly determined by chemical adsorption, which was in line with pseudo-secondary dynamics model. The adsorption was basically homogeneous monolayer adsorption with heat release, which was more agree with Langmuir adsorption isotherm equation. the adsorption process of Ni2+ by MGA. The adsorption process was a spontaneous process and an exothermic reaction. It can be confirmed that the prepared MGA adsorbent can realize slurry separation using magnetic separation principle and has high adsorption capacity to Ni2+.

The Fram Strait integrated ocean observatory

NASA Astrophysics Data System (ADS)

Fahrbach, E.; Beszczynska-Möller, A.; Rettig, S.; Rohardt, G.; Sagen, H.; Sandven, S.; Hansen, E.

2012-04-01

A long-term oceanographic moored array has been operated since 1997 to measure the ocean water column properties and oceanic advective fluxes through Fram Strait. While the mooring line along 78°50'N is devoted to monitoring variability of the physical environment, the AWI Hausgarten observatory, located north of it, focuses on ecosystem properties and benthic biology. Under the EU DAMOCLES and ACOBAR projects, the oceanographic observatory has been extended towards the innovative integrated observing system, combining the deep ocean moorings, multipurpose acoustic system and a network of gliders. The main aim of this system is long-term environmental monitoring in Fram Strait, combining satellite data, acoustic tomography, oceanographic measurements at moorings and glider sections with high-resolution ice-ocean circulation models through data assimilation. In future perspective, a cable connection between the Hausgarten observatory and a land base on Svalbard is planned as the implementation of the ESONET Arctic node. To take advantage of the planned cabled node, different technologies for the underwater data transmission were reviewed and partially tested under the ESONET DM AOEM. The main focus was to design and evaluate available technical solutions for collecting data from different components of the Fram Strait ocean observing system, and an integration of available data streams for the optimal delivery to the future cabled node. The main components of the Fram Strait integrated observing system will be presented and the current status of available technologies for underwater data transfer will be reviewed. On the long term, an initiative of Helmholtz observatories foresees the interdisciplinary Earth-Observing-System FRAM which combines observatories such as the long term deep-sea ecological observatory HAUSGARTEN, the oceanographic Fram Strait integrated observing system and the Svalbard coastal stations maintained by the Norwegian ARCTOS network. A vision of this modular underwater observatory network in Fram Strait will be presented.

FRAM (FRontiers in Arctic marine Monitoring: The FRAM Ocean Observing System) planned efforts for integrated water column biogeochemistry

NASA Astrophysics Data System (ADS)

Nielsdóttir, Maria; Salter, Ian; Kanzow, Torsten; Boetius, Antje

2015-04-01

The Arctic is a region undergoing rapid environmental change and will be subject to multiple stressors in the coming decades. Reductions in sea ice concentration; warming, increased terrigenous inputs and Atlantification are all expected to exert a significant impact on the structure and function of Arctic ecosystems. The Fram Strait is a particularly important region because it acts as a gateway in the exchange of Atlantic and Arctic water masses. The logistical constraints in conducting year round biogeochemical measurements in such areas impose a significant limitation to our understanding of these complicated ecosystems. To address these important challenges the German ministry of research has funded a multi-million Euro infrastructure project (FRAM). Over the next five years FRAM will develop a remote access and autonomous sampling infrastructure to improve the temporal and spatial resolution of biogeochemical measurements in the Fram Strait and central Arctic. Here we present a summary of sampling strategies, technological innovations and biogeochemical parameters that will be addressed over the duration of the project. Specific emphasis will be placed on platforms for monitoring nutrient dynamics, carbonate chemistry, organic carbon flux and the development of a sustained microbial observatory.

Changes in the Composition of the Fram Strait Freshwater Outflow

NASA Astrophysics Data System (ADS)

Dodd, Paul; Granskog, Mats; Fransson, Agneta; Chierici, Melissa; Stedmon, Colin

2016-04-01

Fram Strait is the largest gateway and only deep connection between the Arctic Ocean and the subpolar oceans. Monitoring the exchanges through Fram Strait allows us to detect and understand current changes occurring in the Arctic Ocean and to predict the effects of those changes on the Arctic and Subarctic climate and ecosystems. Polar water, recirculating Atlantic Water and deeper water masses exported from the Arctic Ocean through western Fram Strait are monitored year-round by an array of moored instruments along 78°50'N, continuously maintained by the Norwegian Polar Institute since the 1990s. Complimentary annual hydrographic sections have been repeated along the same latitude every September. This presentation will focus on biogeochemical tracer measurements collected along repeated sections from 1997-2015, which can be used to identify freshwater from different sources and reveal the causes of variations in total volume of freshwater exported e. g.: pulses of freshwater from the Pacific. Repeated tracer sections across Fram Strait reveal significant changes in the composition of the outflow in recent years, with recent sections showing positive fractions of sea ice meltwater at the surface near the core of the EGC, suggesting that more sea ice melts back into the surface than previously. The 1997-2015 time series of measurements reveals a strong anti-correlation between run-off and net sea ice meltwater inventories, suggesting that run-off and brine may be delivered to Fram Strait together from a common source. While the freshwater outflow at Fram Strait typically exhibits a similar run-off to net sea ice meltwater ratio to the central Arctic Ocean and Siberian shelves, we find that the ratio of run-off to sea ice meltwater at Fram Strait is decreasing with time, suggesting an increased surface input of sea ice meltwater in recent years. In 2014 and 2015 measurements of salinity, δ18O and total alkalinity were collected from sea ice cores as well as the underlying water column in Fram Strait. We use this dataset to investigate the feasibility of using concurrent δ18O and total alkalinity measurements to separately identify precipitation, which probably makes up a significant fraction of the freshwater in Fram Strait, but has so far not been separately monitored.

MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical Polycomb repressive complex PRC1.6

PubMed Central

Stielow, Bastian; Finkernagel, Florian; Stiewe, Thorsten

2018-01-01

Diverse Polycomb repressive complexes 1 (PRC1) play essential roles in gene regulation, differentiation and development. Six major groups of PRC1 complexes that differ in their subunit composition have been identified in mammals. How the different PRC1 complexes are recruited to specific genomic sites is poorly understood. The Polycomb Ring finger protein PCGF6, the transcription factors MGA and E2F6, and the histone-binding protein L3MBTL2 are specific components of the non-canonical PRC1.6 complex. In this study, we have investigated their role in genomic targeting of PRC1.6. ChIP-seq analysis revealed colocalization of MGA, L3MBTL2, E2F6 and PCGF6 genome-wide. Ablation of MGA in a human cell line by CRISPR/Cas resulted in complete loss of PRC1.6 binding. Rescue experiments revealed that MGA recruits PRC1.6 to specific loci both by DNA binding-dependent and by DNA binding-independent mechanisms. Depletion of L3MBTL2 and E2F6 but not of PCGF6 resulted in differential, locus-specific loss of PRC1.6 binding illustrating that different subunits mediate PRC1.6 loading to distinct sets of promoters. Mga, L3mbtl2 and Pcgf6 colocalize also in mouse embryonic stem cells, where PRC1.6 has been linked to repression of germ cell-related genes. Our findings unveil strikingly different genomic recruitment mechanisms of the non-canonical PRC1.6 complex, which specify its cell type- and context-specific regulatory functions. PMID:29381691

Assessment of laryngeal muscle and testicular cell types in Xenopus laevis (Anura Pipidae) inhabiting maize and non-maize growing areas of South Africa

USGS Publications Warehouse

Smith, E.E.; Du Preez, L.H.; Gentles, A.; Solomon, K.R.; Tandler, B.; Carr, J.A.; Van Der Kraak, G. L.; Kendall, R.J.; Giesy, J.P.; Gross, T.S.

2005-01-01

We tested the hypothesis that adult African clawed frogs (Xenopus laevis) inhabiting water bodies in maize-growing areas (MGA) of South Africa would exhibit differences in testicular structure compared to frogs from water bodies in non-maize-growing areas (NMGA) in the same locale. Adults of both sexes were collected during the autumn of 2002 in South Africa, and stereological analytical techniques were used to quantify the distribution of testicular cell types. In addition, total laryngeal mass was used as a gauge of secondary sex differences in animals from MGA and NMGA study sites. Evaluation of the total laryngeal mass revealed that there were no statistically significant differences between X. laevis of the same sex from the NMGA and MGA sites. Mean percent fractional-volume values for seminiferous tubule distribution of testicular cell types of mature X. laevis, ranged from 3-4% for spermatogonia, 26-28% for spermatocytes, 54-57% for spermatozoa, and 14-15% for other cells types. The mean percent volume for blood vessels ranged from 0.3-0.4%. These values did not differ significantly between frogs from NMGA and MGA areas. Collectively, these data demonstrated no differences in gonadal and laryngeal development in X. laevis collected in South Africa from MGA and NMGA areas and that there is little evidence for an effect of agricultural chemicals used in maize production functioning as endocrine disrupters in this species. Screening of X. laevis testes revealed a small incidence of Stage 1 testicular oocytes in adult male frogs collected from the NMGA (3%) and MGA (2%).

Ecotoxicological impact of Zequanox®, a novel biocide, on selected non-target Irish aquatic species.

PubMed

Meehan, Sara; Shannon, Adam; Gruber, Bridget; Rackl, Sarahann M; Lucy, Frances E

2014-09-01

Effective, species-specific zebra mussel control is needed urgently for Ireland׳s freshwater bodies, which became infested with non-native zebra mussels in the 1990s. Zequanox®, a newly commercialized product for zebra and quagga mussel control, is composed of dead Pseudomonas fluorescens CL 145A cells. This paper describes ecotoxicology tests on three representative native Irish freshwater species: Anodonta (duck mussel), Chironomus plumosus (non-biting midge), and Austropotamobius pallipes (white-clawed crayfish). The species were exposed to Zequanox in a 72-h static renewal toxicity test at concentrations of 100-750mg active ingredient per liter (mga.i./L). Water quality parameters were measured every 12-24h before and after water and product renewal. After 72h, endpoints were reported as LC10, LC50, and LC100. The LC50 values derived were (1) Anodonta: ≥500mga.i./L (2) C. plumosus: 1075mga.i./L, and (3) A. pallipes: ≥750mga.i./L. These results demonstrate that Zequanox does not negatively affect these organisms at the concentration required for >80percent zebra mussel mortality (150mg a.i/L) and the maximum allowable treatment concentration in the United Sates (200mga.i./L). They also show the overall species-specificity of Zequanox, and support its use in commercial facilities and open waters. Copyright © 2014 Elsevier Inc. All rights reserved.

Conceptual compression discussion on a multi-linear (FTA) and systematic (FRAM) method in an offshore operation's accident modeling.

PubMed

Toroody, Ahmad Bahoo; Abaei, Mohammad Mahdy; Gholamnia, Reza

2016-12-01

Risk assessment can be classified into two broad categories: traditional and modern. This paper is aimed at contrasting the functional resonance analysis method (FRAM) as a modern approach with the fault tree analysis (FTA) as a traditional method, regarding assessing the risks of a complex system. Applied methodology by which the risk assessment is carried out, is presented in each approach. Also, FRAM network is executed with regard to nonlinear interaction of human and organizational levels to assess the safety of technological systems. The methodology is implemented for lifting structures deep offshore. The main finding of this paper is that the combined application of FTA and FRAM during risk assessment, could provide complementary perspectives and may contribute to a more comprehensive understanding of an incident. Finally, it is shown that coupling a FRAM network with a suitable quantitative method will result in a plausible outcome for a predefined accident scenario.

Water use and quality of fresh surface-water resources in the Barataria-Terrebonne Basins, Louisiana

USGS Publications Warehouse

Johnson-Thibaut, Penny M.; Demcheck, Dennis K.; Swarzenski, Christopher M.; Ensminger, Paul A.

1998-01-01

Approximately 170 Mgal/d (million gallons per day) of ground- and surface-water was withdrawn from the Barataria-Terrebonne Basins in 1995. Of this amount, surface water accounted for 64 percent ( 110 MgaVd) of the total withdrawal rates in the basins. The largest surface-water withdrawal rates were from Bayou Lafourche ( 40 Mgal/d), Bayou Boeuf ( 14 MgaVd), and the Gulf Intracoastal Waterway (4.2 Mgal/d). The largest ground-water withdrawal rates were from the Mississippi River alluvial aquifer (29 Mgal/d), the Gonzales-New Orleans aquifer (9.5 Mgal/d), and the Norco aquifer (3.6 MgaVd). The amounts of water withdrawn in the basins in 1995 differed by category of use. Public water suppliers within the basins withdrew 41 Mgal/d of water. The five largest public water suppliers in the basins withdrew 30 Mgal/d of surface water: Terrebonne Waterworks District 1 withdrew the largest amount, almost 15 MgaVd. Industrial facilities withdrew 88 Mgal/d, fossil-fuel plants withdrew 4.7 MgaVd, and commercial facilities withdrew 0.67 MgaVd. Aggregate water-withdrawal rates, compiled by parish for aquaculture (37 Mgal/d), livestock (0.56 Mgal/d), rural domestic (0.44 MgaVd), and irrigation uses (0.54 MgaVd), totaled about 38 MgaVd in the basins. Ninety-five percent of aquaculture withdrawal rates, primarily for crawfish and alligator farming, were from surface-water sources. >br> Total water-withdrawal rates increased 221 percent from 1960–95. Surface-water withdrawal rates have increased by 310 percent, and ground-water withdrawal rates have increased by 133 percent. The projection for the total water-withdrawal rates in 2020 is 220 MgaVd, an increase of 30 percent from 1995. Surface-water withdrawal rates would account for 59 percent of the total, or 130 Mgal/d. Surface-water withdrawal rates are projected to increase by 20 percent from 1995 to 2020. Analysis of water-quality data from the Mississippi River indicates that the main threats to surface water resources are from the herbicide atrazine and excessive nutrients. Atrazine concentrations in the Mississippi River at Baton Rouge briefly exceed the U.S. Environmental Protection Agency maximum contaminant level of 3.0 micrograms per liter during periods in the late spring and early summer. Trace metals in bottom material collected from Bayou Lafourche indicate that the reach of Bayou Lafourche from Donaldsonville to Golden Meadow is adversely affected by low-level contamination. Dissolved nitrate had a mean concentration of 1.4 milligrams per liter in the Mississippi River near Bayou Lafourche and can contribute to excessive plant growth. Long-term salinity records near Bayou Lafourche indicate no pronounced trends, with the exception of the Gulf Intracoastal Waterway at Houma. At this site, salinities remained low until 1961, when the Gulf Intracoastal Waterway was connected to the Gulf of Mexico by the Houma Navigation Canal. The sources of saltwater are variable. Some saltwater has entered Bayou Lafourche south of the Gulf Intracoastal Waterway; at other times saltwater has moved up the Houma Navigation Canal and has flowed east in the Gulf Intracoastal Waterway, north into Company Canal, and southeast in Bayou Lafourche towards Larose, Louisiana.

Heterogeneous Optimization Framework: Reproducible Preprocessing of Multi-Spectral Clinical MRI for Neuro-Oncology Imaging Research.

PubMed

Milchenko, Mikhail; Snyder, Abraham Z; LaMontagne, Pamela; Shimony, Joshua S; Benzinger, Tammie L; Fouke, Sarah Jost; Marcus, Daniel S

2016-07-01

Neuroimaging research often relies on clinically acquired magnetic resonance imaging (MRI) datasets that can originate from multiple institutions. Such datasets are characterized by high heterogeneity of modalities and variability of sequence parameters. This heterogeneity complicates the automation of image processing tasks such as spatial co-registration and physiological or functional image analysis. Given this heterogeneity, conventional processing workflows developed for research purposes are not optimal for clinical data. In this work, we describe an approach called Heterogeneous Optimization Framework (HOF) for developing image analysis pipelines that can handle the high degree of clinical data non-uniformity. HOF provides a set of guidelines for configuration, algorithm development, deployment, interpretation of results and quality control for such pipelines. At each step, we illustrate the HOF approach using the implementation of an automated pipeline for Multimodal Glioma Analysis (MGA) as an example. The MGA pipeline computes tissue diffusion characteristics of diffusion tensor imaging (DTI) acquisitions, hemodynamic characteristics using a perfusion model of susceptibility contrast (DSC) MRI, and spatial cross-modal co-registration of available anatomical, physiological and derived patient images. Developing MGA within HOF enabled the processing of neuro-oncology MR imaging studies to be fully automated. MGA has been successfully used to analyze over 160 clinical tumor studies to date within several research projects. Introduction of the MGA pipeline improved image processing throughput and, most importantly, effectively produced co-registered datasets that were suitable for advanced analysis despite high heterogeneity in acquisition protocols.

Regulation of yeast fatty acid desaturase in response to iron deficiency.

PubMed

Romero, Antonia María; Jordá, Tania; Rozès, Nicolas; Martínez-Pastor, María Teresa; Puig, Sergi

2018-06-01

Unsaturated fatty acids (UFA) are essential components of phospholipids that greatly contribute to the biophysical properties of cellular membranes. Biosynthesis of UFAs relies on a conserved family of iron-dependent fatty acid desaturases, whose representative in the model yeast Saccharomyces cerevisiae is Ole1. OLE1 expression is tightly regulated to adapt UFA biosynthesis and lipid bilayer properties to changes in temperature, and in UFA or oxygen availability. Despite iron deficiency being the most extended nutritional disorder worldwide, very little is known about the mechanisms and the biological relevance of fatty acid desaturases regulation in response to iron starvation. In this report, we show that endoplasmic reticulum-anchored transcription factor Mga2 activates OLE1 transcription in response to nutritional and genetic iron deficiencies. Cells lacking MGA2 display low UFA levels and do not grow under iron-limited conditions, unless UFAs are supplemented or OLE1 is overexpressed. The proteasome, E3 ubiquitin ligase Rsp5 and the Cdc48 Npl4/Ufd1 complex are required for OLE1 activation during iron depletion. Interestingly, Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations. These results reveal the mechanism of OLE1 regulation when iron is scarce and identify the MGA2 auto-regulation as a potential activation strategy in multiple stresses. Copyright © 2018 Elsevier B.V. All rights reserved.

A high throughput screen identifies benzoquinoline compounds as inhibitors of Ebola virus replication.

PubMed

Luthra, Priya; Liang, Jue; Pietzsch, Colette A; Khadka, Sudip; Edwards, Megan R; Wei, Shuguang; De, Sampriti; Posner, Bruce; Bukreyev, Alexander; Ready, Joseph M; Basler, Christopher F

2018-02-01

Ebola virus (EBOV) is an enveloped negative-sense, single-stranded RNA virus of the filovirus family that causes severe disease in humans. Approved therapies for EBOV disease are lacking. EBOV RNA synthesis is carried out by a virus-encoded complex with RNA-dependent RNA polymerase activity that is required for viral propagation. This complex and its activities are therefore potential antiviral targets. To identify potential lead inhibitors of EBOV RNA synthesis, a library of small molecule compounds was screened against a previously established assay of EBOV RNA synthesis, the EBOV minigenome assay (MGA), in 384 well microplate format. The screen identified 56 hits that inhibited EBOV MGA activity by more than 70% while exhibiting less than 20% cell cytotoxicity. Inhibitory chemical scaffolds included angelicin derivatives, derivatives of the antiviral compound GSK983 and benzoquinolines. Structure-activity relationship (SAR) studies of the benzoquinoline scaffold produced ∼50 analogs and led to identification of an optimized compound, SW456, with a submicromolar IC 50 in the EBOV MGA and antiviral activity against infectious EBOV in cell culture. The compound was also active against a MGA for another deadly filovirus, Marburg virus. It also exhibited antiviral activity towards a negative-sense RNA virus from the rhabdovirus family, vesicular stomatitis virus, and a positive-sense RNA virus, Zika virus. Overall, these data demonstrate the potential of the EBOV MGA to identify anti-EBOV compounds and identifies the benzoquinoline series as a broad-spectrum antiviral lead. Copyright © 2017. Published by Elsevier B.V.

Fram Strait sea ice outflow

NASA Technical Reports Server (NTRS)

Kwok, R.; Cunningham, G. F.; Pang, S. S.

2004-01-01

We summarize 24 years (1978??2) of ice export estimates and examine, over a 9-year record, the associated variability in the time-varying upward-looking sonar (ULS) thickness distributions of the Fram Strait.

The Functional Resonance Analysis Method for a systemic risk based environmental auditing in a sinter plant: A semi-quantitative approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Patriarca, Riccardo, E-mail: riccardo.patriarca@uniroma1.it; Di Gravio, Giulio; Costantino, Francesco

Environmental auditing is a main issue for any production plant and assessing environmental performance is crucial to identify risks factors. The complexity of current plants arises from interactions among technological, human and organizational system components, which are often transient and not easily detectable. The auditing thus requires a systemic perspective, rather than focusing on individual behaviors, as emerged in recent research in the safety domain for socio-technical systems. We explore the significance of modeling the interactions of system components in everyday work, by the application of a recent systemic method, i.e. the Functional Resonance Analysis Method (FRAM), in order tomore » define dynamically the system structure. We present also an innovative evolution of traditional FRAM following a semi-quantitative approach based on Monte Carlo simulation. This paper represents the first contribution related to the application of FRAM in the environmental context, moreover considering a consistent evolution based on Monte Carlo simulation. The case study of an environmental risk auditing in a sinter plant validates the research, showing the benefits in terms of identifying potential critical activities, related mitigating actions and comprehensive environmental monitoring indicators. - Highlights: • We discuss the relevance of a systemic risk based environmental audit. • We present FRAM to represent functional interactions of the system. • We develop a semi-quantitative FRAM framework to assess environmental risks. • We apply the semi-quantitative FRAM framework to build a model for a sinter plant.« less

Evaluation of Data Retention Characteristics for Ferroelectric Random Access Memories (FRAMs)

NASA Technical Reports Server (NTRS)

Sharma, Ashok K.; Teverovsky, Alexander

2001-01-01

Data retention and fatigue characteristics of 64 Kb lead zirconate titanate (PZT)-based Ferroelectric Random Access Memories (FRAMs) microcircuits manufactured by Ramtron were examined over temperature range from -85 C to +310 C for ceramic packaged parts and from -85 C to +175 C for plastic parts, during retention periods up to several thousand hours. Intrinsic failures, which were caused by a thermal degradation of the ferroelectric cells, occurred in ceramic parts after tens or hundreds hours of aging at temperatures above 200 C. The activation energy of the retention test failures was 1.05 eV and the extrapolated mean-time-to-failure (MTTF) at room temperature was estimated to be more than 280 years. Multiple write-read cycling (up to 3x10(exp 7)) during the fatigue testing of plastic and ceramic parts did not result in any parametric or functional failures. However, operational currents linearly decreased with the logarithm of number of cycles thus indicating fatigue process in PZT films. Plastic parts, that had more recent date code as compared to ceramic parts, appeared to be using die with improved process technology and showed significantly smaller changes in operational currents and data access times.

Along-slope variability of barotropic transport in the Nordic Seas: Simplified dynamics tested against observations

NASA Astrophysics Data System (ADS)

Aaboe, S.; NøSt, O. A.; Hansen, E.

2009-03-01

The present study combines simple geostrophic considerations and observational data to examine the along-slope evolution of the barotropic transport following the continental slope in the Nordic Seas and Arctic Ocean. Following geostrophic theory, the transport evolution is calculated from bottom densities in five different transects within the Nordic Seas. This theoretically predicted transport evolution is compared with transports estimated directly from velocity measurements. Between the Faroe-Shetland Channel and the Svinøy section off southern Norway, across the Fram Strait (below 800 m), and between the Fram Strait and the Greenland Sea the agreement is generally good between geostrophic theory and observations. This indicates that many aspects of the barotropic flow in these regions are captured by geostrophic dynamics. Between the eastern and western Fram Strait above 800 m and between Svinøy and the eastern Fram Strait the observed flow fields differ to a large extent from the flow field predicted by geostrophic dynamics, indicating that ageostrophic dynamics play a more important role in these regions. The barotropic transport differences between the eastern and western Fram Strait contain information about the barotropic transport evolution along the entire Arctic continental slope. The good agreement between observations and theory across the strait (below 800 m) therefore indicates that the flow along the deeper part of the Arctic continental slope is well described by geostrophic dynamics. According to our results, the noticeably more baroclinic nature of the flow in the western Fram Strait, compared with the eastern strait, can then be ascribed to water mass modifications along the Arctic continental slope.

Remodeling of the Nuclear Envelope and Lamina during Bovine Preimplantation Development and Its Functional Implications

PubMed Central

Popken, Jens; Graf, Alexander; Krebs, Stefan; Blum, Helmut; Schmid, Volker J.; Strauss, Axel; Guengoer, Tuna; Zakhartchenko, Valeri; Wolf, Eckhard; Cremer, Thomas

2015-01-01

The present study demonstrates a major remodeling of the nuclear envelope and its underlying lamina during bovine preimplantation development. Up to the onset of major embryonic genome activation (MGA) at the 8-cell stage nuclei showed a non-uniform distribution of nuclear pore complexes (NPCs). NPCs were exclusively present at sites where DNA contacted the nuclear lamina. Extended regions of the lamina, which were not contacted by DNA, lacked NPCs. In post-MGA nuclei the whole lamina was contacted rather uniformly by DNA. Accordingly, NPCs became uniformly distributed throughout the entire nuclear envelope. These findings shed new light on the conditions which control the integration of NPCs into the nuclear envelope. The switch from maternal to embryonic production of mRNAs was accompanied by multiple invaginations covered with NPCs, which may serve the increased demands of mRNA export and protein import. Other invaginations, as well as interior nuclear segments and vesicles without contact to the nuclear envelope, were exclusively positive for lamin B. Since the abundance of these invaginations and vesicles increased in concert with a massive nuclear volume reduction, we suggest that they reflect a mechanism for fitting the nuclear envelope and its lamina to a shrinking nuclear size during bovine preimplantation development. In addition, a deposit of extranuclear clusters of NUP153 (a marker for NPCs) without associated lamin B was frequently observed from the zygote stage up to MGA. Corresponding RNA-Seq data revealed deposits of spliced, maternally provided NUP153 mRNA and little unspliced, newly synthesized RNA prior to MGA, which increased strongly at the initiation of embryonic expression of NUP153 at MGA. PMID:25932910

Remodeling of the Nuclear Envelope and Lamina during Bovine Preimplantation Development and Its Functional Implications.

PubMed

Popken, Jens; Graf, Alexander; Krebs, Stefan; Blum, Helmut; Schmid, Volker J; Strauss, Axel; Guengoer, Tuna; Zakhartchenko, Valeri; Wolf, Eckhard; Cremer, Thomas

2015-01-01

The present study demonstrates a major remodeling of the nuclear envelope and its underlying lamina during bovine preimplantation development. Up to the onset of major embryonic genome activation (MGA) at the 8-cell stage nuclei showed a non-uniform distribution of nuclear pore complexes (NPCs). NPCs were exclusively present at sites where DNA contacted the nuclear lamina. Extended regions of the lamina, which were not contacted by DNA, lacked NPCs. In post-MGA nuclei the whole lamina was contacted rather uniformly by DNA. Accordingly, NPCs became uniformly distributed throughout the entire nuclear envelope. These findings shed new light on the conditions which control the integration of NPCs into the nuclear envelope. The switch from maternal to embryonic production of mRNAs was accompanied by multiple invaginations covered with NPCs, which may serve the increased demands of mRNA export and protein import. Other invaginations, as well as interior nuclear segments and vesicles without contact to the nuclear envelope, were exclusively positive for lamin B. Since the abundance of these invaginations and vesicles increased in concert with a massive nuclear volume reduction, we suggest that they reflect a mechanism for fitting the nuclear envelope and its lamina to a shrinking nuclear size during bovine preimplantation development. In addition, a deposit of extranuclear clusters of NUP153 (a marker for NPCs) without associated lamin B was frequently observed from the zygote stage up to MGA. Corresponding RNA-Seq data revealed deposits of spliced, maternally provided NUP153 mRNA and little unspliced, newly synthesized RNA prior to MGA, which increased strongly at the initiation of embryonic expression of NUP153 at MGA.

Frequency-tunable terahertz absorber with wire-based metamaterial and graphene

NASA Astrophysics Data System (ADS)

Xiong, Han; Jiang, Yan-Nan; Yang, Cheng; Zeng, Xiao-Ping

2018-01-01

We present a dynamically tunable metamaterial graphene absorber (MGA) in the terahertz regime. The unit cell of the proposed MGA consists of metal wire and graphene sheet over the grounded dielectric absorber. The MGA achieves frequency tunable characteristics via changing the chemical potential. In order to understand the absorption mechanism of this absorber, a simple equivalent circuit method has been proposed. Because the coupling between wire-based metamaterial and graphene is complicated and cannot be neglected an equivalent surface impedance was introduced and extracted for simplification. In addition to the chemical potential of graphene, the constitutive parameters of metal wire are also discussed in detail to completely understand how these factors affect the absorption properties. It is believed that this study may be useful for providing valuable guidance in the development of more advanced MGAs.

Organic additives stabilize RNA aptamer binding of malachite green.

PubMed

Zhou, Yubin; Chi, Hong; Wu, Yuanyuan; Marks, Robert S; Steele, Terry W J

2016-11-01

Aptamer-ligand binding has been utilized for biological applications due to its specific binding and synthetic nature. However, the applications will be limited if the binding or the ligand is unstable. Malachite green aptamer (MGA) and its labile ligand malachite green (MG) were found to have increasing apparent dissociation constants (Kd) as determined through the first order rate loss of emission intensity of the MGA-MG fluorescent complex. The fluorescent intensity loss was hypothesized to be from the hydrolysis of MG into malachite green carbinol base (MGOH). Random screening organic additives were found to reduce or retain the fluorescence emission and the calculated apparent Kd of MGA-MG binding. The protective effect became more apparent as the percentage of organic additives increased up to 10% v/v. The mechanism behind the organic additive protective effects was primarily from a ~5X increase in first order rate kinetics of MGOH→MG (kMGOH→MG), which significantly changed the equilibrium constant (Keq), favoring the generation of MG, versus MGOH without organic additives. A simple way has been developed to stabilize the apparent Kd of MGA-MG binding over 24h, which may be beneficial in stabilizing other triphenylmethane or carbocation ligand-aptamer interactions that are susceptible to SN1 hydrolysis. Copyright © 2016 Elsevier B.V. All rights reserved.

mga genosensor for early detection of human rheumatic heart disease.

PubMed

Singh, Swati; Kaushal, Ankur; Khare, Shashi; Kumar, Ashok

2014-05-01

The 5' amino-labeled DNA probe complementary to mga gene of Streptococcus pyogenes was immobilized on carboxylated multiwall carbon nanotubes electrode and hybridized with 0.1-100 ng/6 μl single-stranded genomic DNA (ssG-DNA) of S. pyogenes from throat swab of suspected rheumatic heart disease (RHD) patients. Electrochemical response was measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance (EI). The sensitivity of the sensor was 106.03 (μA/cm(2))/ng and limit of detection (LOD) was found 0.014 ng/6 μl with regression coefficient (R(2)) of 0.921 using DPV. The genosensor was characterized by FTIR and SEM, and electrode was found stable for 6 months on storage at 4 °C with 5-6 % loss in initial DPV current. mga genosensor is the first report on RHD sensor which can save life of several suspected patients by early diagnosis in 30 min.

Annual Report on the Activities and Publications of the DHS-DNDO-NTNFC Sponsored Post-doctoral Fellow at Los Alamos National Laboratory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rim, Jung Ho; Tandon, Lav

This report is a summary of the projects Jung Rim is working on as a DHS postdoctoral fellow at Los Alamos National Laboratory. These research projects are designed to explore different radioanalytical methods to support nuclear forensics applications. The current projects discussed here include development of alpha spectroscopy method for 240/239Pu Isotopic ratio measurement, non-destructive uranium assay method using gamma spectroscopy, and 236U non-destructive uranium analysis using FRAM code. This report documents the work that has been performed since the start of the postdoctoral appointment.

Developmental profiles of progesterone receptor transcripts and molecular responses to gestagen exposure during Silurana tropicalis early development.

PubMed

Thomson, Paisley; Langlois, Valerie S

2018-05-18

Environmental gestagens are an emerging class of contaminants that have been recently measured in surface water and can interfere with reproduction in aquatic vertebrates. Gestagens include endogenous progestogens, such as progesterone (P4), which bind P4-receptors and have critically important roles in vertebrate physiology and reproduction. Gestagens also include synthetic progestins, which are components of human and veterinary drugs, such as melengestrol acetate (MGA). Endogenous progestogens are essential in the regulation of reproduction in mammalian species, but the role of P4 in amphibian larval development remains unclear. This project aims to understand the roles and the regulatory mechanisms of P4 in amphibians and to assess the consequences of exposures to environmental gestagens on the P4-receptor signaling pathways in frogs. Here, we established the developmental profiles of the P4 receptors: the intracellular progesterone receptor (ipgr), the membrane progesterone receptor β (mpgrβ), and the progesterone receptor membrane component 1 (pgrmc1) in Western clawed frog (Silurana tropicalis) embryos using real-time qPCR. P4-receptor mRNAs were detected throughout embryogenesis. Transcripts for ipgr and pgrmc1 were detected in embryos at Nieuwkoop and Faber (NF) stage 2 and 7, indicative of maternal transfer of mRNA. We also assessed the effects of P4 and MGA exposure in embryonic and early larval development. Endocrine responses were evaluated through transcript analysis of a suite of gene targets of interest, including: ipgr, mpgrβ, pgrmc1, androgen receptor (ar), estrogen receptor α (erα), follicle stimulating hormone β (fshβ), prolactin (prl), and the steroid 5-alpha reductase family (srd5α1, 2, and 3). Acute exposure (NF 12-46) to P4 caused a 2- to 5-fold change increase of ipgr, mpgrβ, pgrmc1, and ar mRNA levels at the environmentally relevant concentration of 195 ng/L P4. Acute exposure to MGA induced a 56% decrease of srd5α3 at 1140 ng/L MGA. We conclude that environmental exposure to P4 induced multiple endocrine-related transcript responses in amphibians; however, the differential responses of MGA suggest that the effects of MGA are not mediated through the classical P4 signaling pathway in S. tropicalis. Copyright © 2018 Elsevier Inc. All rights reserved.

'Fram' in Color

NASA Technical Reports Server (NTRS)

2004-01-01

[figure removed for brevity, see original site] Click on the image for 'Fram' in Color (QTVR)

This view in approximately true color reveals details in an impact crater informally named 'Fram' in the Meridian Planum region of Mars. The picture is a mosaic of frames taken by the panoramic camera on NASA's Mars Exploration Rover Opportunity during the rover's 88th martian day on Mars, on April 23, 2004. The crater spans about 8 meters (26 feet) in diameter. Opportunity paused beside it while traveling from the rover's landing site toward a larger crater farther east. This view combines images taken using three of the camera's filters for different wavelengths of light: 750 nanometers, 530 nanometers and 430 nanometers.

Magnetic Gearing Versus Conventional Gearing in Actuators for Aerospace Applications

NASA Technical Reports Server (NTRS)

Puchhammer, Gregor

2014-01-01

Magnetic geared actuators (MGA) are designed to perform highly reliable, robust and precise motion on satellite platforms or aerospace vehicles. The design allows MGA to be used for various tasks in space applications. In contrast to conventional geared drives, the contact and lubrication free force transmitting elements lead to a considerable lifetime and range extension of drive systems. This paper describes the fundamentals of magnetic wobbling gears (MWG) and the deduced inherent characteristics, and compares conventional and magnetic gearing.

"Failure-to-Identify" Hunting Incidents: A Resilience Engineering Approach.

PubMed

Bridges, Karl E; Corballis, Paul M; Hollnagel, Erik

2018-03-01

Objective The objective was to develop an understanding, using the Functional Resonance Analysis Method (FRAM), of the factors that could cause a deer hunter to misidentify their intended target. Background Hunting is a popular activity in many communities. However, hunters vary considerably based on training, experience, and expertise. Surprisingly, safety in hunting has not received much attention, especially failure-to-identify hunting incidents. These are incidents in which the hunter, after spotting and targeting their quarry, discharge their firearm only to discover they have been spotting and targeting another human, an inanimate object, or flora by mistake. The hunter must consider environment, target, time of day, weather, and many other factors-continuously evaluating whether the hunt should continue. To understand how these factors can relate to one another is fundamental to begin to understand how incidents happen. Method Workshops with highly experienced and active hunters led to the development of a FRAM model detailing the functions of a "Hunting FRAM." The model was evaluated for correctness based on confidential and anonymous near-miss event submissions by hunters. Results A FRAM model presenting the functions of a hunt was produced, evaluated, and accepted. Using the model, potential sources of incidents or other unintended outcomes were identified, which in turn helped to improve the model. Conclusion Utilizing principles of understanding and visualization tools of the FRAM, the findings create a foundation for safety improvements potentially through training or safety messages based on an increased understanding of the complexity of hunting.

Methane excess production in oxygen-rich polar water and a model of cellular conditions for this paradox

NASA Astrophysics Data System (ADS)

Damm, E.; Thoms, S.; Beszczynska-Möller, A.; Nöthig, E. M.; Kattner, G.

2015-09-01

Summer sea ice cover in the Arctic Ocean has undergone a reduction in the last decade exposing the sea surface to unforeseen environmental changes. Melting sea ice increases water stratification and induces nutrient limitation, which is also known to play a crucial role in methane formation in oxygenated surface water. We report on an excess of methane in the marginal ice zone in the western Fram Strait. Our study is based on measurements of oxygen, methane, DMSP, nitrate and phosphate concentrations as well as on phytoplankton composition and light transmission, conducted along the 79°N oceanographic transect, in the western part of the Fram Strait and in Northeast Water Polynya region off Greenland. Between the eastern Fram Strait, where Atlantic water enters from the south and the western Fram Strait, where Polar water enters from the north, different nutrient limitations occurred and consequently different bloom conditions were established. Ongoing sea ice melting enhances the environmental differences between both water masses and initiates regenerated production in the western Fram Strait. We show that in this region methane is in situ produced while DMSP (dimethylsulfoniopropionate) released from sea ice may serve as a precursor for the methane formation. The methane production occured despite high oxygen concentrations in this water masses. As the metabolic activity (respiration) of unicellular organisms explains the presence of anaerobic conditions in the cellular environment we present a theoretical model which explains the maintenance of anaerobic conditions for methane formation inside bacterial cells, despite enhanced oxygen concentrations in the environment.

Biogeography and Photosynthetic Biomass of Arctic Marine Pico-Eukaroytes during Summer of the Record Sea Ice Minimum 2012

PubMed Central

Metfies, Katja; von Appen, Wilken-Jon; Kilias, Estelle; Nicolaus, Anja; Nöthig, Eva-Maria

2016-01-01

Information on recent photosynthetic biomass distribution and biogeography of Arctic marine pico-eukaryotes (0.2–3 μm) is needed to better understand consequences of environmental change for Arctic marine ecosystems. We analysed pico-eukaryote biomass and community composition in Fram Strait and large parts of the Central Arctic Ocean (Nansen Basin, Amundsen Basin) using chlorophyll a (Chl a) measurements, automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing. Samples were collected during summer 2012, the year with the most recent record sea ice minimum. Chl a concentrations were highest in eastern Fram Strait and pico-plankton accounted for 60–90% of Chl a biomass during the observation period. ARISA-patterns and 454-pyrosequencing revealed that pico-eukaryote distribution is closely related to water mass distribution in the euphotic zone of the Arctic Ocean. Phaeocystaceae, Micromonas sp., Dinophyceae and Syndiniales constitute a high proportion of sequence reads, while sequence abundance of autotrophic Phaeocystaceae and mixotrophic Micromonas sp. was inversely correlated. Highest sequence abundances of Phaeocystaceae were observed in the warm Atlantic Waters in Fram Strait, while Micromonas sp. dominated the abundant biosphere in the arctic halocline. Our results are of particular interest considering existing hypotheses that environmental conditions in Nansen Basin might become more similar to the current conditions in Fram Strait. We propose that in response, biodiversity and biomass of pico-eukaryotes in Nansen Basin could resemble those currently observed in Fram Strait in the future. This would significantly alter biogeochemical cycles in a large part of the Central Arctic Ocean. PMID:26895333

Results from On-Orbit Testing of the Fram Memory Test Experiment on the Fastsat Micro-Satellite

NASA Technical Reports Server (NTRS)

MacLeod, Todd C.; Sims, W. Herb; Varnavas, Kosta A.; Ho, Fat D.

2011-01-01

NASA is planning on going beyond Low Earth orbit with manned exploration missions. The radiation environment for most Low Earth orbit missions is harsher than at the Earth's surface but much less harsh than deep space. Development of new electronics is needed to meet the requirements of high performance, radiation tolerance, and reliability. The need for both Volatile and Non-volatile memory has been identified. Emerging Non-volatile memory technologies (FRAM, C-RAM,M-RAM, R-RAM, Radiation Tolerant FLASH, SONOS, etc.) need to be investigated for use in Space missions. An opportunity arose to fly a small memory experiment on a high inclination satellite (FASTSAT). An off-the-shelf 512K Ramtron FRAM was chosen to be tested in the experiment.

Limitation and facilitation of one of the world's most invasive fish: an intercontinental comparison

USGS Publications Warehouse

Budy, Phaedra E.; Thiede, Gary P.; Lobón-Cerviá, Javier; Fernandez, Gustavo Gonzolez; McHugh, Peter; McIntosh, Angus; Vøllestad, Lief Asbjørn; Becares, Eloy; Jellyman, Phillip

2013-01-01

Purposeful species introductions offer opportunities to inform our understanding of both invasion success and conservation hurdles. We evaluated factors determining the energetic limitations of brown trout (Salmo trutta) in both their native and introduced ranges. Our focus was on brown trout because they are nearly globally distributed, considered one of the world's worst invaders, yet imperiled in much of their native habitat. We synthesized and compared data describing temperature regime, diet, growth, and maximum body size across multiple spatial and temporal scales, from country (both exotic and native habitats) and major geographic area (MGA) to rivers and years within MGA. Using these data as inputs, we next used bioenergetic efficiency (BioEff), a relative scalar representing a realized percentage of maximum possible consumption (0–100%) as our primary response variable and a multi-scale, nested, mixed statistical model (GLIMMIX) to evaluate variation among and within spatial scales and as a function of density and elevation. MGA and year (the residual) explained the greatest proportion of variance in BioEff. Temperature varied widely among MGA and was a strong driver of variation in BioEff. We observed surprisingly little variation in the diet of brown trout, except the overwhelming influence of the switch to piscivory observed only in exotic MGA. We observed only a weak signal of density-dependent effects on BioEff; however, BioEff remained 2.5 fish/m2. The trajectory of BioEff across the life span of the fish elucidated the substantial variation in performance among MGAs; the maximum body size attained by brown trout was consistently below 400 mm in native habitat but reached 600 mm outside their native range, where brown trout grew rapidly, feeding in part on naive prey fishes. The integrative, physiological approach, in combination with the intercontinental and comparative nature of our study, allowed us to overcome challenges associated with context-dependent variation in determining invasion success. Overall our results indicate “growth plasticity across the life span” was important for facilitating invasion, and should be added to lists of factors characterizing successful invaders.

Streptococcus iniae M-Like Protein Contributes to Virulence in Fish and Is a Target for Live Attenuated Vaccine Development

PubMed Central

Locke, Jeffrey B.; Aziz, Ramy K.; Vicknair, Mike R.; Nizet, Victor; Buchanan, John T.

2008-01-01

Background Streptococcus iniae is a significant pathogen in finfish aquaculture, though knowledge of virulence determinants is lacking. Through pyrosequencing of the S. iniae genome we have identified two gene homologues to classical surface-anchored streptococcal virulence factors: M-like protein (simA) and C5a peptidase (scpI). Methodology/Principal Findings S. iniae possesses a Mga-like locus containing simA and a divergently transcribed putative mga-like regulatory gene, mgx. In contrast to the Mga locus of group A Streptococcus (GAS, S. pyogenes), scpI is located distally in the chromosome. Comparative sequence analysis of the Mgx locus revealed only one significant variant, a strain with an insertion frameshift mutation in simA and a deletion mutation in a region downstream of mgx, generating an ORF which may encode a second putative mga-like gene, mgx2. Allelic exchange mutagenesis of simA and scpI was employed to investigate the potential role of these genes in S. iniae virulence. Our hybrid striped bass (HSB) and zebrafish models of infection revealed that M-like protein contributes significantly to S. iniae pathogenesis whereas C5a peptidase-like protein does not. Further, in vitro cell-based analyses indicate that SiMA, like other M family proteins, contributes to cellular adherence and invasion and provides resistance to phagocytic killing. Attenuation in our virulence models was also observed in the S. iniae isolate possessing a natural simA mutation. Vaccination of HSB with the ΔsimA mutant provided 100% protection against subsequent challenge with a lethal dose of wild-type (WT) S. iniae after 1,400 degree days, and shows promise as a target for live attenuated vaccine development. Conclusions/Significance Analysis of M-like protein and C5a peptidase through allelic replacement revealed that M-like protein plays a significant role in S. iniae virulence, and the Mga-like locus, which may regulate expression of this gene, has an unusual arrangement. The M-like protein mutant created in this research holds promise as live-attenuated vaccine. PMID:18665241

Exposure and effects of sediment-spiked fludioxonil on macroinvertebrates and zooplankton in outdoor aquatic microcosms.

PubMed

Yin, Xiao H; Brock, Theo C M; Barone, Lidia E; Belgers, J Dick M; Boerwinkel, Marie-Claire; Buijse, Laura; van Wijngaarden, René P A; Hamer, Mick; Roessink, Ivo

2018-01-01

Information from effects of pesticides in sediments at an ecosystem level, to validate current and proposed risk assessment procedures, is scarce. A sediment-spiked outdoor freshwater microcosm experiment was conducted with fludioxonil (lipophilic, non-systemic fungicide) to study exposure dynamics and treatment-related responses of benthic and pelagic macroinvertebrates and zooplankton. Besides blank control and solvent control systems the experiment had six different treatment levels (1.7-614mga.s./kg dry sediment) based around the reported 28-d No Observed Effect Concentration (NOEC) for Chironomus riparius (40mga.s./kg dry sediment). Twelve systems were available per treatment of which four were sacrificed on each of days 28, 56 and 84 after microcosm construction. Fludioxonil persisted in the sediment and mean measured concentrations were 53-82% of the initial concentration after 84days. The dissipation rate increased with the treatment level. Also exposure concentrations in overlying water were long-term, with highest concentrations 28days after initiation of the experiment. Sediment-dwelling Oligochaeta and pelagic Rotifera and Cladocera showed the most pronounced treatment-related declines. The most sensitive sediment-dwelling oligochaete was Dero digitata (population NOEC 14.2mga.s./kg dry sediment). The same NOEC was calculated for the sediment-dwelling macroinvertebrate community. The most sensitive zooplankton species was the cladoceran Diaphanosoma brachyurum (NOEC of 1.6μga.s./L in overlying water corresponding to 5.0mga.s./kg dry sediment). At the two highest treatments several rotifer taxa showed a pronounced decrease, while the zooplankton community-level NOEC was 5.6μga.s./L (corresponding to 14.2mga.s./kg dry sediment). Zooplankton taxa calanoid Copepoda and Daphnia gr. longispina showed a pronounced treatment-related increase (indirect effects). Consequently, an assessment factor of 10 to the chronic laboratory NOECs of Chironomus riparius (sediment) and Daphnia magna (water) results in a regulatory acceptable concentration that is sufficiently protective for both the sediment-dwelling and pelagic organisms in the microcosms. Copyright © 2017 Elsevier B.V. All rights reserved.

Variability of Fram Strait Ice Flux and North Atlantic Oscillation

NASA Technical Reports Server (NTRS)

Kwok, Ron

1999-01-01

An important term in the mass balance of the Arctic Ocean sea ice is the ice export. We estimated the winter sea ice export through the Fram Strait using ice motion from satellite passive microwave data and ice thickness data from moored upward looking sonars. The average winter area flux over the 18-year record (1978-1996) is 670,000 square km, approximately 7% of the area of the Arctic Ocean. The winter area flux ranges from a minimum of 450,000 sq. km in 1984 to a maximum of 906,000 sq km in 1995. The daily, monthly and interannual variabilities of the ice area flux are high. There is an upward trend in the ice area flux over the 18-year record. The average winter volume flux over the winters of October 1990 through May 1995 is 1745 cubic km ranging from a low of 1375 cubic km in 1990 to a high of 2791 cubic km in 1994. The sea-level pressure gradient across the Fram Strait explains more than 80% of the variance in the ice flux over the 18-year record. We use the coefficients from the regression of the time-series of area flux versus pressure gradient across the Fram Strait and ice thickness data to estimate the summer area and volume flux. The average 12-month area flux and volume flux are 919,000 sq km and 2366 cubic km. We find a significant correlation (R =0.86) between the area flux and positive phases of the North Atlantic Oscillation (NAO) index over the months of December through March. Correlation between our six years of volume flux estimates and the NAO index gives R =0.56. During the high NAO years, a more intense Icelandic low increases the gradient in the sea-level pressure by almost 1 mbar across the Fram Strait thus increasing the atmospheric forcing on ice transport. Correlation is reduced during the negative NAO years because of decreased dominance of this large-scale atmospheric pattern on the sea-level pressure gradient across the Fram Strait. Additional information is contained in the original.

The Regulatory Small RNA MarS Supports Virulence of Streptococcus pyogenes.

PubMed

Pappesch, Roberto; Warnke, Philipp; Mikkat, Stefan; Normann, Jana; Wisniewska-Kucper, Aleksandra; Huschka, Franziska; Wittmann, Maja; Khani, Afsaneh; Schwengers, Oliver; Oehmcke-Hecht, Sonja; Hain, Torsten; Kreikemeyer, Bernd; Patenge, Nadja

2017-09-25

Small regulatory RNAs (sRNAs) play a role in the control of bacterial virulence gene expression. In this study, we investigated an sRNA that was identified in Streptococcus pyogenes (group A Streptococcus, GAS) but is conserved throughout various streptococci. In a deletion strain, expression of mga, the gene encoding the multiple virulence gene regulator, was reduced. Accordingly, transcript and proteome analyses revealed decreased expression of several Mga-activated genes. Therefore, and because the sRNA was shown to interact with the 5' UTR of the mga transcript in a gel-shift assay, we designated it MarS for m ga-activating regulatory sRNA. Down-regulation of important virulence factors, including the antiphagocytic M-protein, led to increased susceptibility of the deletion strain to phagocytosis and reduced adherence to human keratinocytes. In a mouse infection model, the marS deletion mutant showed reduced dissemination to the liver, kidney, and spleen. Additionally, deletion of marS led to increased tolerance towards oxidative stress. Our in vitro and in vivo results indicate a modulating effect of MarS on virulence gene expression and on the pathogenic potential of GAS.

Evaluation of Data Retention and Imprint Characteristics of FRAMs Under Environmental Stresses for NASA Applications

NASA Technical Reports Server (NTRS)

Sharma, Ashok K.; Teverovsky, Alexander; Dowdy, Terry W.; Hamilton, Brett

2000-01-01

A major reliability issue for all advanced nonvolatile memory (NVM) technology devices including FRAMs (Ferroelectric random access memories) is the data retention characteristics over extended period of time, under environmental stresses and exposure to total ionizing dose (TID) radiation effects. For this testing, 256 Kb FRAMs in 28-pin plastic DIPS, rated for industrial grade temperature range of -40 C to +85 C, were procured. These are two-transistor, two-capacitor (2T-2C) design FRAMs. In addition to data retention characteristics, the parts were also evaluated for imprint failures, which are defined as the failure of cells to change from a "preferred" state, where it has been for a significant period of time to an opposite state (e.g., from 1 to 0, or 0 to 1). These 256 K FRAMs were subjected to scanning acoustic microscopy (C-SAM); 1,000 temperature cycles from -65 C to +150 C; high temperature aging at 150 C, 175 C, and 200 C for 1,000 hours; highly accelerated stress test (HAST) for 500 hours; 1,000 hours of operational life test at 125 C; and total ionizing dose radiation testing. As a preconditioning, 10 K read/write cycles were performed on all devices. Interim electrical measurements were performed throughout this characterization, including special imprint testing and final electrical testing. Some failures were observed during high temperature aging test at 200 C, during HAST testing, and during 1,000 hours of operational life at 125 C. The parts passed 10 Krad exposure, but began showing power supply current increases during the dose increment from 10 Krad to 30 Krad, and at 40 Krad severe data retention and parametric failures were observed. Failures from various environmental group testing are currently being analyzed.

Evaluation of Data Retention and Imprint Characteristics of FRAMs Under Environmental Stresses for NASA Applications

NASA Technical Reports Server (NTRS)

Sharma, Asbok K.; Teverovsky, Alexander; Dowdy, Terry W.; Hamilton, Brett

2002-01-01

A major reliability issue for all advanced nonvolatile memory (NVM) technology devices including FRAMs is the data retention characteristics over extended period of time, under environmental stresses and exposure to total ionizing dose (TID) radiation effects. For this testing, 256 Kb FRAMs in 28-pin plastic DIPS, rated for industrial grade temperature range of -40 C to +85 C, were procured. These are two-transistor, two-capacitor (2T-2C) design FRAMs. In addition to data retention characteristics, the parts were also evaluated for imprint failures, which are defined as the failure of cells to change from a "preferred" state, where it has been for a significant period of time to an opposite state (e.g., from 1 to 0, or 0 to 1). These 256 K FRAMs were subjected to scanning acoustic microscopy (C-SAM); 1,000 temperature cycles from -65 C to +150 C; high temperature aging at 150 C, 175 C, and 200 C for 1,000 hours; highly accelerated stress test (HAST) for 500 hours; 1,000 hours of operational life test at 125 C; and total ionizing dose radiation testing. As a preconditioning, 10 K read/write cycles were performed on all devices. Interim electrical measurements were performed throughout this characterization, including special imprint testing and final electrical testing. Some failures were observed during high temperature aging test at 200 C, during HAST testing, and during 1,000 hours of operational life at 125 C. The parts passed 10 Krad exposure, but began showing power supply current increases during the dose increment from 10 Krad to 30 Krad, and at 40 Krad severe data retention and parametric failures were observed. Failures from various environmental group testing are currently being analyzed.

Transsphenoidal Surgery for Mixed Pituitary Gangliocytoma-Adenomas.

PubMed

Shepard, Matthew J; Elzoghby, Mohamed A; Ghanim, Daffer; Lopes, M Beatriz S; Jane, John A

2017-12-01

Most sellar gangliocytomas are discovered with a concurrent pituitary adenoma, also known as a mixed gangliocytoma-adenoma (MGA). MGAs are rare, with fewer than 100 cases reported in the literature to date and only 1 previously documented surgical series. Because MGAs are radiologically indistinguishable from pituitary adenomas, they are often diagnosed after surgery. Combined with the paucity of clinical outcome data for these tumors, this makes their diagnosis and management challenging. Here we describe the clinical presentation and outcomes of 10 individuals who were diagnosed with a MGA at a single institution. This retrospective case series study included patients diagnosed with a combined sellar MGA between 1993 and 2016. This series comprised 10 patients, mean age of 44 years (range, 28-63 years) diagnosed with an MGA. The mean tumor size was 1.6 cm (range, 0.4-2.4 cm). Five patients presented with acromegaly, and 1 patient had recurrent Cushing disease. Transsphenoidal surgery was performed in all cases, and gross total resection was achieved in 7 patients (70%). Histologically, 9 of the 10 MGAs were identified as mixed somatotroph adenoma-gangliocytomas. The median duration of follow-up was 74 months (range, 2-180 months). Following adjuvant treatment (n = 3), all patients with acromegaly (n = 4) achieved biochemical remission, and no patient experienced recurrence of the pituitary tumor with a median radiographic follow-up of 48 months. MGAs are often associated with a hypersecretory adenoma. Transsphenoidal surgery is well tolerated by most patients, and when performed in combination with adjuvant therapy, a low rate of recurrence and reversal of preoperative endocrinopathy can be expected. Copyright © 2017 Elsevier Inc. All rights reserved.

Fram Strait sea ice outflow

NASA Technical Reports Server (NTRS)

Kwok, R.; Cunningham, G. F.; Pang, S. S.

2004-01-01

We summarize 24 years of ice export estimates and examine, over a 9-year record, the associated variability in the time-varying upward-looking sonar (ULS) thickness distributions of the Fram Strait. A more thorough assessment of the PMW (passive microwave) ice motion with 5 years of synthetic aperture radar (SAR)observations shows the uncertainties to be consistent with that found by Kwok and Rothrock [1999], giving greater confidence to the record of ice flux calculations.

An investigation of messy genetic algorithms

NASA Technical Reports Server (NTRS)

Goldberg, David E.; Deb, Kalyanmoy; Korb, Bradley

1990-01-01

Genetic algorithms (GAs) are search procedures based on the mechanics of natural selection and natural genetics. They combine the use of string codings or artificial chromosomes and populations with the selective and juxtapositional power of reproduction and recombination to motivate a surprisingly powerful search heuristic in many problems. Despite their empirical success, there has been a long standing objection to the use of GAs in arbitrarily difficult problems. A new approach was launched. Results to a 30-bit, order-three-deception problem were obtained using a new type of genetic algorithm called a messy genetic algorithm (mGAs). Messy genetic algorithms combine the use of variable-length strings, a two-phase selection scheme, and messy genetic operators to effect a solution to the fixed-coding problem of standard simple GAs. The results of the study of mGAs in problems with nonuniform subfunction scale and size are presented. The mGA approach is summarized, both its operation and the theory of its use. Experiments on problems of varying scale, varying building-block size, and combined varying scale and size are presented.

TRISTEN/FRAM II Cruise Report, East Arctic, April 1980.

DTIC Science & Technology

1981-04-13

is not readily accessible by air from Alaska. The Eurasia Basin contains the Arctic Midoceanic Ridge, which extends in a straight line for 2000 km...13 6 Bottom Refraction - Shot- Lines Overlain on FRAM II Positions 14 7 Waterfall Display of Successive Spectral Estimates of Single...Northeast leg of the array was oriented 341T and the NW leg 304 ’T. After a windstorm and flow break-up on 16 April, hydrophones 11 and 12 and 21-24 were

Programmable Logic Application Notes

NASA Technical Reports Server (NTRS)

Katz, Richard

2000-01-01

This column will be provided each quarter as a source for reliability, radiation results, NASA capabilities, and other information on programmable logic devices and related applications. This quarter will continue a series of notes concentrating on analysis techniques with this issue's section discussing: Digital Timing Analysis Tools and Techniques. Articles in this issue include: SX and SX-A Series Devices Power Sequencing; JTAG and SXISX-AISX-S Series Devices; Analysis Techniques (i.e., notes on digital timing analysis tools and techniques); Status of the Radiation Hard reconfigurable Field Programmable Gate Array Program, Input Transition Times; Apollo Guidance Computer Logic Study; RT54SX32S Prototype Data Sets; A54SX32A - 0.22 micron/UMC Test Results; Ramtron FM1608 FRAM; and Analysis of VHDL Code and Synthesizer Output.

Evidence that multiple genetic variants of MC4R play a functional role in the regulation of energy expenditure and appetite in Hispanic children1234

PubMed Central

Cole, Shelley A; Voruganti, V Saroja; Cai, Guowen; Haack, Karin; Kent, Jack W; Blangero, John; Comuzzie, Anthony G; McPherson, John D; Gibbs, Richard A

2010-01-01

Background: Melanocortin-4-receptor (MC4R) haploinsufficiency is the most common form of monogenic obesity; however, the frequency of MC4R variants and their functional effects in general populations remain uncertain. Objective: The aim was to identify and characterize the effects of MC4R variants in Hispanic children. Design: MC4R was resequenced in 376 parents, and the identified single nucleotide polymorphisms (SNPs) were genotyped in 613 parents and 1016 children from the Viva la Familia cohort. Measured genotype analysis (MGA) tested associations between SNPs and phenotypes. Bayesian quantitative trait nucleotide (BQTN) analysis was used to infer the most likely functional polymorphisms influencing obesity-related traits. Results: Seven rare SNPs in coding and 18 SNPs in flanking regions of MC4R were identified. MGA showed suggestive associations between MC4R variants and body size, adiposity, glucose, insulin, leptin, ghrelin, energy expenditure, physical activity, and food intake. BQTN analysis identified SNP 1704 in a predicted micro-RNA target sequence in the downstream flanking region of MC4R as a strong, probable functional variant influencing total, sedentary, and moderate activities with posterior probabilities of 1.0. SNP 2132 was identified as a variant with a high probability (1.0) of exerting a functional effect on total energy expenditure and sleeping metabolic rate. SNP rs34114122 was selected as having likely functional effects on the appetite hormone ghrelin, with a posterior probability of 0.81. Conclusion: This comprehensive investigation provides strong evidence that MC4R genetic variants are likely to play a functional role in the regulation of weight, not only through energy intake but through energy expenditure. PMID:19889825

The pressure-temperature phase diagram of pressure induced organic superconductors β-(BDA-TTP){2}MCl{4} (M = Ga, Fe)

NASA Astrophysics Data System (ADS)

Choi, E. S.; Graf, D.; Brooks, J. S.; Yamada, J.; Tokumoto, M.

2004-04-01

We investigate the pressure-temperature phase diagram of β -(BDA-TTP){2}MCl{4} (M=Ga, Fe), which shows a metal-insulator (MI) transition around 120 K at ambient pressure. By applying pressure, the insulating phase is suppressed. When the pressure is higher than 5.5 kbar, the superconducting phase appears in both salts with Tc ˜ 3 K for M=Ga and 2.2 K for M=Fe. We also observed Shubnikov-de Haas (SdH) oscillations at high magnetic field in both salts, where the SdH frequencies are found to be very similar each other. Key words. organic superconductor, pressure, phase diagram.

The potential impact of multidimesional geriatric assessment in the social security system.

PubMed

Corbi, Graziamaria; Ambrosino, Immacolata; Massari, Marco; De Lucia, Onofrio; Simplicio, Sirio; Dragone, Michele; Paolisso, Giuseppe; Piccioni, Massimo; Ferrara, Nicola; Campobasso, Carlo Pietro

2018-01-12

To evaluate the efficacy of multidimensional geriatric assessment (MGA/CGA) in patients over 65 years old in predicting the release of the accompaniment allowance (AA) indemnity by a Local Medico-Legal Committee (MLC-NHS) and by the National Institute of Social Security Committee (MLC-INPS). In a longitudinal observational study, 200 Italian elder citizens requesting AA were first evaluated by MLC-NHS and later by MLC-INPS. Only MLC-INPS performed a MGA/CGA (including SPMSQ, Barthel Index, GDS-SF, and CIRS). This report was written according to the STROBE guidelines. The data analysis was performed on January 2016. The evaluation by the MLC-NHS and by the MLC-INPS was in agreement in 66% of cases. In the 28%, the AA benefit was recognized by the MLC-NHS, but not by the MLC-INPS. By the multivariate analysis, the best predictors of the AA release, by the MLC-NHS, were represented by gender and the Barthel Index score. The presence of carcinoma, the Barthel Index score, and the SPMQ score were the best predictors for the AA release by MLC-INPS. MGA/CGA could be useful in saving financial resources reducing the risk of incorrect indemnity release. It can improve the accuracy of the impairment assessment in social security system.

Core pathways operating during methylotrophy of Bacillus methanolicus MGA3 and induction of a bacillithiol-dependent detoxification pathway upon formaldehyde stress.

PubMed

Müller, Jonas E N; Meyer, Fabian; Litsanov, Boris; Kiefer, Patrick; Vorholt, Julia A

2015-12-01

Bacillus methanolicus MGA3 is a model facultative methylotroph of interest for fundamental research and biotechnological applications. Previous research uncovered a number of pathways potentially involved in one-carbon substrate utilization. Here, we applied dynamic (13) C labeling to elucidate which of these pathways operate during growth on methanol and to uncover potentially new ones. B. methanolicus MGA3 uses the assimilatory and dissimilatory ribulose monophosphate (RuMP) cycles for conversion of the central but toxic intermediate formaldehyde. Additionally, the operation of two cofactor-dependent formaldehyde oxidation pathways with distinct roles was revealed. One is dependent on tri- and tetraglutamylated tetrahydrofolate (THF) and is involved in formaldehyde oxidation during growth on methanol. A second pathway was discovered that is dependent on bacillithiol, a thiol cofactor present also in other Bacilli where it is known to function in redox-homeostasis. We show that bacillithiol-dependent formaldehyde oxidation is activated upon an upshift in formaldehyde induced by a substrate switch from mannitol to methanol. The genes and the corresponding enzymes involved in the biosynthesis of bacillithiol were identified by heterologous production of bacillithiol in Escherichia coli. The presented results indicate metabolic plasticity of the methylotroph allowing acclimation to fluctuating intracellular formaldehyde concentrations. © 2015 John Wiley & Sons Ltd.

First oceanographic observations on the Wandel Sea shelf in Northeast Greenland: Tracing the Arctic Ocean outflow through the western Fram Strait

NASA Astrophysics Data System (ADS)

Dmitrenko, Igor A.; Kirillov, Sergei A.; Rudels, Bert; Babb, David G.; Pedersen, Leif T.; Rysgaard, Soeren; Kristoffersen, Yngve; Barber, David G.

2016-04-01

The first-ever conductivity-temperature-depth (CTD) observations on the Wandel Sea shelf in North Eastern Greenland were collected from the land-fast ice in April-May 2015 as a part of the Arctic Science Partnership collaboration during the first research campaign at the Villum Research Station. They were complemented by (i) the ice-tethered profiler (ITP) and Acoustic Dopler Current Profiler (ADCP) mooring observations in ~300 m of the tidewater glacier outlet from the Flade Isblink Ice Cap and (ii) CTDs taken in June-July 2015 along the Wandel Sea continental slope during the Norwegian FRAM 2014-15 sea ice drift. The CTD profiles deeper than 100 m are used to reveal the origin of water masses and determine the extent to which these water masses have interacted with ambient water from the continental slope. The subsurface water layer from ~20-70 m depth is comprised of freshened water (30-32 psu) that is likely associated with the Pacific Water outflow from the Arctic Ocean through the western Fram Strait. The underlying halocline layer centered at ~80 m (~33 psu) separates the Pacific Water layer from a deeper (<140 m) layer of modified Polar Water that has interacted with the warm Atlantic Water outflow through Fram Strait. The Atlantic Water layer with temperature above 0°C is recorded below 140 m. Over the outer shelf, the halocline layer shows numerous cold density-compensated intrusions indicating lateral interaction with an ambient Polar Water mass across the continental slope. Mooring data shows an enhanced shelf-slope interaction responding the storm event in 23-24 April 2015 with northerly winds exceeding 10 m/s. The on-shelf transport of a cold and turbid water from the upper continental slope results in enhanced interleaving within the depth range of the halocline layer (~70-100 m). Our observations of Pacific Water in the Wandel Sea subsurface layer are set in the context of upstream observations in the Beaufort Sea for 2002-2011 and downstream observations from the Northeast Water Polynya (1992-1993), and clearly show the modification of Pacific Water during its advection across the Arctic Ocean from the Bering Strait to Fram Strait. Moreover, the Wandel Sea shelf and continental slope water shows a different water mass structure indicating the different origin and pathways of the on-shore and off-shore branches of the Arctic Ocean outflow through the Western Fram Strait.

Mechanism of the hydroxyl radical oxidation of methacryloyl peroxynitrate (MPAN) and its pathway toward secondary organic aerosol formation in the atmosphere

DOE PAGES

Nguyen, Tran B.; Bates, Kelvin H.; Crounse, John D.; ...

2015-06-12

Methacryloyl peroxynitrate (MPAN), the acyl peroxynitrate of methacrolein, has been suggested to be an important secondary organic aerosol (SOA) precursor from isoprene oxidation. Yet, the mechanism by which MPAN produces SOA through reaction with the hydroxyl radical (OH) is unclear. We systematically evaluate three proposed mechanisms in controlled chamber experiments and provide the first experimental support for the theoretically-predicted lactone formation pathway from the MPAN + OH reaction, producing hydroxymethyl-methyl-α-lactone (HMML). The decomposition of the MPAN–OH adduct yields HMML + NO 3 (~75%) and hydroxyacetone + CO + NO 3 (~25%), out-competing its reaction with atmospheric oxygen. The production ofmore » other proposed SOA precursors, e.g., methacrylic acid epoxide (MAE), from MPAN and methacrolein are negligible (<2%). Furthermore, we show that the beta-alkenyl moiety of MPAN is critical for lactone formation. Alkyl radicals formed cold via H-abstraction by OH do not decompose to HMML, even if they are structurally identical to the MPAN–OH adduct. The SOA formation from HMML, from polyaddition of the lactone to organic compounds at the particle interface or in the condensed phase, is close to unity under dry conditions. However, the SOA yield is sensitive to particle liquid water and solvated ions. In hydrated inorganic particles, HMML reacts primarily with H 2O to produce the monomeric 2-methylglyceric acid (2MGA) or with aqueous sulfate and nitrate to produce the associated organosulfate and organonitrate, respectively. 2MGA, a tracer for isoprene SOA, is semivolatile and its accommodation in aerosol water decreases with decreasing pH. Conditions that enhance the production of neutral 2MGA suppress SOA mass from the HMML channel. In conclusion, considering the liquid water content and pH ranges of ambient particles, 2MGA will exist largely as a gaseous compound in some parts of the atmosphere.« less

Holocene water mass history off NE Greenland - A first high-resolution sediment record from the western Fram Strait

NASA Astrophysics Data System (ADS)

Zehnich, Marc; Palme, Tina; Spielhagen, Robert F.; Hass, H. Christian; Bauch, Henning A.

2017-04-01

While the Holocene history of the eastern Fram Strait seems well investigated, no high-resolution paleoenvironmental records were available from the western Fram Strait so far. A new sedimentary record, obtained during expedition PS93.1 (2015) of RV Polarstern on the outermost NE Greenland shelf, allows for the first time to reconstruct Holocene changes in near-surface salinities, temperature, stratification and water masses (polar waters vs. Atlantic Water), potentially related to variations of the freshwater and sea ice export from the Arctic Ocean. The 260 cm long sedimentary record from site PS93/025 (80.5°N, 8.5°W) was investigated for sediment composition, foraminifer contents, grain size variations (sortable silt) and the isotopic composition of planktic foraminifers. Radiocarbon datings reveal an age of 10.2 cal-ka for the core base and continuous sedimentation throughout most of the Holocene. The sediments are generally very fine-grained (<2% sand). The grain size record reveals a fining-upwards trend and sediments from <6.5 cal-ka consist of <0.5% coarse fraction. A comparison of foraminifer and coarse fraction abundances shows strong similarities. Apparently the contribution of coarse terrestrial material from iceberg transport was extremely low throughout the last 10.2 cal-ka. Foraminifer abundances (both planktic and benthic) are high in Early Holocene sediments until ca. 7 cal-ka and decrease rapidly thereafter. This is interpreted to reflect a relatively strong advection of Atlantic Water to the NW Fram Strait, which correlates well with similar findings on the eastern side of the Arctic Gateway. Sortable silt grain sizes are high (27-32 µm) in the older part of the record and gradually decrease between 7 cal-ka and 4 cal-ka. After ca. 4 cal-ka, sortable silt shows values of 20-22 µm and little variation. Considering also the grain-size distribution curves, we propose a decline of bottom current velocities on the outer NE Greenland shelf after 7 cal-ka, related to a decrease of Atlantic Water advection. These preliminary results reveal a strong coupling of Holocene environments on both sides of the Fram Strait.

Impacts of Changed Extratropical Storm Tracks on Arctic Sea Ice Export through Fram Strait

NASA Astrophysics Data System (ADS)

Wei, J.; Zhang, X.; Wang, Z.

2017-12-01

Studies have indicated a poleward shift of extratropical storm tracks and intensification of Arctic storm activities, in particular on the North Atlantic side of the Arctic Ocean. To improve understanding of dynamic effect on changes in Arctic sea ice mass balance, we examined the impacts of the changed storm tracks and activities on Arctic sea ice export through Fram Strait through ocean-sea ice model simulations. The model employed is the high-resolution Massachusetts Institute of Technology general circulation model (MITgcm), which was forced by the Japanese 25-year Reanalysis (JRA-25) dataset. The results show that storm-induced strong northerly wind stress can cause simultaneous response of daily sea ice export and, in turn, exert cumulative effects on interannual variability and long-term changes of sea ice export. Further analysis indicates that storm impact on sea ice export is spatially dependent. The storms occurring southeast of Fram Strait exhibit the largest impacts. The weakened intensity of winter storms in this region after 1994/95 could be responsible for the decrease of total winter sea ice export during the same time period.

Characteristics of Milk Fermented by Streptococcus thermophilus MGA45-4 and the Profiles of Associated Volatile Compounds during Fermentation and Storage.

PubMed

Dan, Tong; Jin, Rulin; Ren, Weiyi; Li, Ting; Chen, Haiyan; Sun, Tiansong

2018-04-11

The lactic acid bacterium Streptococcus thermophilus is a major starter culture for the production of dairy products. In this study, the physiochemical characteristics of milk fermented by the MGA45-4 isolate of S. thermophilus were analyzed. Our data indicate that milk fermented using S. thermophilus MGA45-4 maintained a high viable cell count (8.86 log10 colony-forming units/mL), and a relatively high pH (4.4), viscosity (834.33 mPa·s), and water holding capacity (40.85%) during 14 days of storage. By analyzing the volatile compound profile using solid-phase microextraction and gas chromatography/mass spectrometry, we identified 73 volatile compounds in the fermented milk product, including five carboxylic acids, 21 aldehydes, 13 ketones, 16 alcohols, five esters, and 13 aromatic carbohydrates. According to the odor activity values, 11 of these volatile compounds were found to play a key role in producing the characteristic flavor of fermented milk, particularly octanal, nonanal, hexanal, 2,3-butanedione, and 1-octen-3-ol, which had the highest odor activity values among all compounds analyzed. These findings thus provide more insights in the chemical/molecular characteristics of milk fermented using S. thermophilus , which may provide a basis for improving dairy product flavor/odor during the process of fermentation and storage.

Emergency Communications for NASA's Deep Space Missions

NASA Technical Reports Server (NTRS)

Shambayati, Shervin; Lee, Charles H.; Morabito, David D.; Cesarone, Robert J.; Abraham, Douglas S.

2011-01-01

The ability to communicate with spacecraft during emergencies is a vital service that NASA's Deep Space Network (DSN) provides to all deep space missions. Emergency communications is characterized by low data rates(typically is approximately10 bps) with the spacecraft using either a low-gain antenna (LGA, including omnidirectional antennas) or,in some cases, a medium-gain antenna (MGA). Because of the use of LGAs/MGAs for emergency communications, the transmitted power requirements both on the spacecraft andon the ground are substantially greater than those required for normal operations on the high-gain antenna (HGA) despite the lower data rates. In this paper, we look at currentand future emergency communications capabilities available to NASA's deep-space missions and discuss their limitations in the context of emergency mode operations requirements.These discussions include the use of the DSN 70-m diameter antennas, the use of the 34-m diameter antennas either alone or arrayed both for the uplink (Earth-to-spacecraft) and the downlink (spacecraft-to-Earth), upgrades to the ground transmitters, and spacecraft power requirements both with unitygain (0 dB) LGAs and with antennas with directivity (>0 dB gain, either LGA or MGA, depending on the gain). Also discussed are the requirements for forward-error-correctingcodes for both the uplink and the downlink. In additional, we introduce a methodology for proper selection of a directionalLGA/MGA for emergency communications.

Spatial and temporal scales of sea ice protists and phytoplankton distribution from the gateway Fram Strait into the Central Arctic Ocean

NASA Astrophysics Data System (ADS)

Peeken, I.; Hardge, K.; Krumpen, T.; Metfies, K.; Nöthig, E. M.; Rabe, B.; von Appen, W. J.; Vernet, M.

2016-02-01

The Arctic Ocean is currently one of the key regions where the effect of climate change is most pronounced. Sea ice is an important interface in this region by representing a unique habitat for many organisms. Massive reduction of sea ice thickness and extent, which have been recorded over the last twenty years, is anticipated to cause large cascading changes in the entire Arctic ecosystem. Most sea ice is formed on the Eurasian shelves and transported via the Transpolardrift to the western Fram Strait and out of the Arctic Ocean with the cold East Greenland Current (EGC). Warm Atlantic water enters the Arctic Ocean with the West Spitsbergen Current (WSC) via eastern Fram Strait. Here, we focus on the spatial spreading of protists from the Atlantic water masses, and their occurrences over the deep basins of the Central Arctic and the relationship amongst them in water and sea ice. Communities were analyzed by using pigments, flow cytometer and ARISA fingerprints during several cruises with the RV Polarstern to the Fram Strait, the Greenland Sea and the Central Arctic Ocean. By comparing these data sets we are able to demonstrate that the origin of the studied sea ice floes is more important for the biodiversity found in the sea ice communities then the respective underlying water mass. In contrast, biodiversity in the water column is mainly governed by the occurring water masses and the presence or absence of sea ice. However, overall the development of standing stocks in both biomes was governed by the availability of nutrients. To get a temporal perspective of the recent results, the study will be embedded in a long-term data set of phytoplankton biomass obtained during several cruises over the last twenty years.

Current Pattern Change in the Fram Strait at the Pliocene/Pleistocene Boundary

NASA Astrophysics Data System (ADS)

Gebhardt, C.; Geissler, W. H.; Matthiessen, J. J.; Jokat, W.

2014-12-01

Thick packages of drift-type sediments were identified in the northwestern and central part of the Fram Strait, mainly along the western Yermak Plateau flank, but also in the central, flat part of the Fram Strait. A large-scale field of sediment waves was found north of 80.5°, along the Yermak Plateau rise. This field separates two drift bodies, a deeper one towards west and a shallower one towards east. The drift bodies were deposited by bottom currents, most likely by the northbound Yermak Branch of the West Spitsbergen Current, but an influence of a southbound current on the westren drift body cannot be ruled out. Within the drift bodies and even more pronounced withing the sediment waves, a stratigraphic boundary is clearly visible. It separates a lower package of waves migrating upslope at a low angle of ~5° from an upper package with significantly increased wave crest migration at ~16.5°. Using the seismic network, this stratigraphic boundary could be tracked to ODP Leg 151, Site 911, where it corresponds to the lithostratigraphic boundary between units IA and IB dated to 2.7 Ma. The increase in wave-crest migration angle points at a shift towards higher sedimentation rates at 2.7 Ma. This corresponds to the intensification of the Northern Hemisphere glaciation with a major expansion of the Scandinavian, northern Barents Sea, North American and Greenland ice sheets. The Barents Shelf that was subaerially exposed and the expansion of the northern Barents Sea ice sheet (as well as Svalbard) are the likely sources for enhanced erosion and fluvial input along the pathway of the West Spitsbergen Current, resulting in higher sedimentation rates in the Fram Strait.

Dissimilation of [(13)C]methanol by continuous cultures of Bacillus methanolicus MGA3 at 50 degrees C studied by (13)C NMR and isotope-ratio mass spectrometry.

PubMed

Pluschkell, Stefanie B; Flickinger, Michael C

2002-10-01

Using a continuous culture of Bacillus methanolicus MGA3 limited by 100 mM methanol in the feed and growing at a dilution rate D=0.25 h(-1), transients in dissolved methanol were studied to determine the effects of methanol toxicity and the pathway of methanol dissimilation to CO(2). Steady-state cultures were disturbed by pulses of methanol resulting in a rapid change in concentration of 6.4-12.8 mM. B. methanolicus MGA3 responded to a sudden increase in available methanol by a transient decline in the biomass concentration in the reactor. In most cases the culture returned to steady state between 4 and 12 h after pulse addition. However, at a methanol pulse of 12.8 mM, complete biomass washout occurred and the culture did not return to steady state. Integrating the response curves of the dry biomass concentration over a 12 h time period showed that a methanol pulse can cause an average transient decline in the biomass yield of up to 22%. (13)C NMR experiments using labelled methanol indicated that the transient partial or complete biomass washout was probably caused by toxic accumulation of formaldehyde in the culture. These experiments also showed accumulation of formate, indicating that B. methanolicus possesses formaldehyde dehydrogenase and formate dehydrogenase activity resulting in a methanol dissimilation pathway via formate to CO(2). Studies using isotope-ratio mass spectrometry provided further evidence of a methanol dissimilation pathway via formate. B. methanolicus MGA3, growing continuously under methanol limitation, consumed added formate at a rate of approximately 0.85 mmol l(-1) h(-1). Furthermore, significant accumulation of (13)CO(2) in the reactor exhaust gas was measured in response to a pulse addition of [(13)C]formic acid to the bioreactor. This indicates that B. methanolicus dissimilates methanol carbon to CO(2) in order to detoxify formaldehyde by both a linear pathway to formate and a cyclic mechanism as part of the RuMP pathway.

Linkages between atmospheric blocking, sea ice export through Fram Strait and the Atlantic Meridional Overturning Circulation

PubMed Central

Ionita, M.; Scholz, P.; Lohmann, G.; Dima, M.; Prange, M.

2016-01-01

As a key persistent component of the atmospheric dynamics, the North Atlantic blocking activity has been linked to extreme climatic phenomena in the European sector. It has also been linked to Atlantic multidecadal ocean variability, but its potential links to rapid oceanic changes have not been investigated. Using a global ocean-sea ice model forced with atmospheric reanalysis data, here it is shown that the 1962–1966 period of enhanced blocking activity over Greenland resulted in anomalous sea ice accumulation in the Arctic and ended with a sea ice flush from the Arctic into the North Atlantic Ocean through Fram Strait. This event induced a significant decrease of Labrador Sea water surface salinity and an abrupt weakening of the Atlantic Meridional Overturning Circulation (AMOC) during the 1970s. These results have implications for the prediction of rapid AMOC changes and indicate that an important part of the atmosphere-ocean dynamics at mid- and high latitudes requires a proper representation of the Fram Strait sea ice transport and of the synoptic scale variability such as atmospheric blocking, which is a challenge for current coupled climate models. PMID:27619955

Linkages between atmospheric blocking, sea ice export through Fram Strait and the Atlantic Meridional Overturning Circulation.

PubMed

Ionita, M; Scholz, P; Lohmann, G; Dima, M; Prange, M

2016-09-13

As a key persistent component of the atmospheric dynamics, the North Atlantic blocking activity has been linked to extreme climatic phenomena in the European sector. It has also been linked to Atlantic multidecadal ocean variability, but its potential links to rapid oceanic changes have not been investigated. Using a global ocean-sea ice model forced with atmospheric reanalysis data, here it is shown that the 1962-1966 period of enhanced blocking activity over Greenland resulted in anomalous sea ice accumulation in the Arctic and ended with a sea ice flush from the Arctic into the North Atlantic Ocean through Fram Strait. This event induced a significant decrease of Labrador Sea water surface salinity and an abrupt weakening of the Atlantic Meridional Overturning Circulation (AMOC) during the 1970s. These results have implications for the prediction of rapid AMOC changes and indicate that an important part of the atmosphere-ocean dynamics at mid- and high latitudes requires a proper representation of the Fram Strait sea ice transport and of the synoptic scale variability such as atmospheric blocking, which is a challenge for current coupled climate models.

Impacts of extratropical storm tracks on Arctic sea ice export through Fram Strait

NASA Astrophysics Data System (ADS)

Wei, Jianfen; Zhang, Xiangdong; Wang, Zhaomin

2018-05-01

Studies have indicated regime shifts in atmospheric circulation, and associated changes in extratropical storm tracks and Arctic storm activity, in particular on the North Atlantic side of the Arctic Ocean. To improve understanding of changes in Arctic sea ice mass balance, we examined the impacts of the changed storm tracks and cyclone activity on Arctic sea ice export through Fram Strait by using a high resolution global ocean-sea ice model, MITgcm-ECCO2. The model was forced by the Japanese 25-year Reanalysis (JRA-25) dataset. The results show that storm-induced strong northerly wind stress can cause simultaneous response of daily sea ice export and, in turn, exert cumulative effects on interannual variability and long-term changes of sea ice export. Further analysis indicates that storm impact on sea ice export is spatially dependent. The storms occurring southeast of Fram Strait exhibit the largest impacts. The weakened intensity of winter (in this study winter is defined as October-March and summer as April-September) storms in this region after 1994/95 could be responsible for the decrease of total winter sea ice export during the same time period.

Ice-tethered measurement platforms in the Arctic Ocean: a contribution by the FRAM infrastructure program

NASA Astrophysics Data System (ADS)

Hoppmann, Mario; Nicolaus, Marcel; Rabe, Benjamin; Wenzhöfer, Frank; Katlein, Christian; Scholz, Daniel

2016-04-01

The Arctic Ocean has been in the focus of many studies during recent years, investigating the state, the causes and the implications of the observed rapid transition towards a thinner and younger sea-ice cover. However, consistent observational datasets of sea ice, ocean and atmosphere are still sparse due to the limited accessibility and harsh environmental conditions. One important tool to fill this gap has become more and more feasible during recent years: autonomous, ice-tethered measurement platforms (buoys). These drifting instruments independently transmit their data via satellites, and enable observations over larger areas and over longer time periods than manned expeditions, even throughout the winter. One aim of the newly established FRAM (FRontiers in Arctic marine Monitoring) infrastructure program at the Alfred-Wegener-Institute is to realize and maintain an interdisciplinary network of buoys in the Arctic Ocean, contributing to an integrated, Arctic-wide observatory. The additional buoy infrastructure, ship-time, and developments provided by FRAM are critical elements in the ongoing international effort to fill the large data gaps in a rapidly changing Arctic Ocean. Our focus is the particularly underrepresented Eurasian Basin. Types of instruments range from snow depth beacons and ice mass balance buoys for monitoring ice growth and snow accumulation, over radiation and weather stations for energy budget estimates, to ice-tethered profiling systems for upper ocean monitoring. Further, development of new bio-optical and biogeochemical buoys is expected to enhance our understanding of bio-physical processes associated with Arctic sea ice. The first set of FRAM buoys was deployed in September 2015 from RV Polarstern. All datasets are publicly available on dedicated web portals. Near real time data are reported into international initiatives, such as the Global Telecommunication System (GTS) and the International Arctic Buoy Programme (IABP). The additional data acquired by FRAM buoys facilitate the validation of model results and remote sensing products, play an important role in understanding the linkages between the atmosphere, sea ice and upper ocean, and help assess the physical, biological and biogeochemical states of the future Arctic Ocean. Here we present our recent work and future plans, but are also aiming for additional collaborations, especially on technical developments, scientific questions and deployment logistics.

Use of the reaction of malachite green with 11-molybdogermanic heteropolyacid

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mirzoyan, F.V.; Airiyan, E.K.; Tarayan, V.M.

1985-05-01

This paper presents the results of an investigation of the interaction of malachite green (MG) with molybdogermanic heteropolyacid (MGA) in a wide range of acidity and concentrations of the reacting componenets in order to establish the optimum conditions of formation and isolation of more highly substituted salts of MGA and to increase the sensitivity of the photometric determination of germanium. It is shown that 11-molybdogermanic acid interacts quantitatively with malachite green in a wide range of acidity, forming three solid phase compounds of different compositions, contining 2, 4, and 8 associated cations of the dye. The compound with composition 8:1more » was used to develop a highly sensitive method of determining submicro- and microgram quantities of germanium after its extraction determination in the form of GeC1/sub 4/.« less

An evolutionary morphological approach for software development cost estimation.

PubMed

Araújo, Ricardo de A; Oliveira, Adriano L I; Soares, Sergio; Meira, Silvio

2012-08-01

In this work we present an evolutionary morphological approach to solve the software development cost estimation (SDCE) problem. The proposed approach consists of a hybrid artificial neuron based on framework of mathematical morphology (MM) with algebraic foundations in the complete lattice theory (CLT), referred to as dilation-erosion perceptron (DEP). Also, we present an evolutionary learning process, called DEP(MGA), using a modified genetic algorithm (MGA) to design the DEP model, because a drawback arises from the gradient estimation of morphological operators in the classical learning process of the DEP, since they are not differentiable in the usual way. Furthermore, an experimental analysis is conducted with the proposed model using five complex SDCE problems and three well-known performance metrics, demonstrating good performance of the DEP model to solve SDCE problems. Copyright © 2012 Elsevier Ltd. All rights reserved.

Synthesis of palladium@gold nanoalloys/nitrogen and sulphur-functionalized multiple graphene aerogel for electrochemical detection of dopamine.

PubMed

Li, Ruiyi; Yang, Tingting; Li, Zaijun; Gu, Zhiguo; Wang, Guangli; Liu, Junkang

2017-02-15

Integration of noble metal nanomaterials on graphene nanosheets potentially paves one way to improve their electronic, chemical and electrochemical properties. The study reported synthesis of palladium@gold nanoalloys/nitrogen and sulphur-functionalized multiple graphene aerogel composite (Pd@Au/N,S-MGA). The as-prepared composite offers a well-defined three-dimensional architecture with rich of mesopores. The Pd@Au nanoalloys were dispersed on the graphene framework networks and their active sites were fully exposed. The unique structure achieves to ultra high electron/ion conductivity, electrocatalytic activity and structural stability. The sensor based on the Pd@Au/N,S-MGA creates ultrasensitive electrochemical response towards dopamine due to significantly electrochemical synergy between Pd, Au and N,S-MGA. Its differential pulse voltammetric signal linearly increases with the increase of dopamine concentration in the range from 1.0 × 10 -9  M to 4.0 × 10 -5  M with the detection limit of 3.6 × 10 -10  M (S/N = 3). The analytical method provides the advantage of sensitivity, reproducibility, rapidity and long-term stability. It has been successfully applied in the detection of trace dopamine in biological samples. The study also opens a window on the electronic properties of graphene aerogel and metal nanomaterials as well their nanohybrids to meet needs of further applications as nanoelectronics in diagnosis, bioanalysis and catalysis. Copyright © 2016 Elsevier B.V. All rights reserved.

Waveform Generator Signal Processing Software

DOT National Transportation Integrated Search

1988-09-01

This report describes the software that was developed to process test waveforms that were recorded by crash test data acquisition systems. The test waveforms are generated by an electronic waveform generator developed by MGA Research Corporation unde...

High quality self-separated GaN crystal grown on a novel nanoporous template by HVPE.

PubMed

Huo, Qin; Shao, Yongliang; Wu, Yongzhong; Zhang, Baoguo; Hu, Haixiao; Hao, Xiaopeng

2018-02-16

In this study, a novel nanoporous template was obtained by a two-step etching process from MOCVD-GaN/Al 2 O 3 (MGA) with electrochemical etching sequentially followed by chemical wet etching. The twice-etched MOCVD-GaN/Al 2 O 3 (TEMGA) templates were utilized to grow GaN crystals by hydride vapor phase epitaxy (HVPE) method. The GaN crystals were separated spontaneously from the TEMGA template with the assistance of voids formed by the etched nanopores. Several techniques were utilized to characterize the quality of the free-standing GaN crystals obtained from the TEMGA template. Results showed that the quality of the as-obtained GaN crystals was improved obviously compared with those grown on the MGA. This convenient technique can be applied to grow high-quality free-standing GaN crystals.

The compositional change of Fluorescent Dissolved Organic Matter across Fram Strait assessed with use of a multi channel in situ fluorometer.

NASA Astrophysics Data System (ADS)

Raczkowska, A.; Kowalczuk, P.; Sagan, S.; Zabłocka, M.; Pavlov, A. K.; Granskog, M. A.; Stedmon, C. A.

2016-02-01

Observations of Colored Dissolved Organic Matter absorption (CDOM) and fluorescence (FDOM) from water samples and an in situ fluorometer and of Inherent Optical Properties (IOP; light absorption and scattering) were carried out along a section across Fram Strait at 79°N. A 3 channel Wetlabs Wetstar fluorometer was deployed, with channels for humic- and protein-like DOM and used to assess distribution of different FDOM fractions. A relationship between fluorescence intensity of the protein-like fraction of FDOM and chlorophyll a fluorescence was found and indicated the importance of phytoplankton biomass in West Spitsbergen Current waters as a significant source of protein-like FDOM. East Greenland Current waters has low concentration of chlorophyll a, and were characterized by high humic-like FDOM fluorescence. An empirical relationship between humic-like FDOM fluorescence intensity and CDOM absorption was derived and confirms the dominance of terrigenous like CDOM on the composition of DOM in the East Greenland Current. These high resolution profile data offer a simple approach to fractionate the contribution of these two DOM source to DOM across the Fram Strait and may help refine estimates of DOC fluxes in and out of the Arctic through this region.

Using fluorescent dissolved organic matter to trace and distinguish the origin of Arctic surface waters

PubMed Central

Gonçalves-Araujo, Rafael; Granskog, Mats A.; Bracher, Astrid; Azetsu-Scott, Kumiko; Dodd, Paul A.; Stedmon, Colin A.

2016-01-01

Climate change affects the Arctic with regards to permafrost thaw, sea-ice melt, alterations to the freshwater budget and increased export of terrestrial material to the Arctic Ocean. The Fram and Davis Straits represent the major gateways connecting the Arctic and Atlantic. Oceanographic surveys were performed in the Fram and Davis Straits, and on the east Greenland Shelf (EGS), in late summer 2012/2013. Meteoric (fmw), sea-ice melt, Atlantic and Pacific water fractions were determined and the fluorescence properties of dissolved organic matter (FDOM) were characterized. In Fram Strait and EGS, a robust correlation between visible wavelength fluorescence and fmw was apparent, suggesting it as a reliable tracer of polar waters. However, a pattern was observed which linked the organic matter characteristics to the origin of polar waters. At depth in Davis Strait, visible wavelength FDOM was correlated to apparent oxygen utilization (AOU) and traced deep-water DOM turnover. In surface waters FDOM characteristics could distinguish between surface waters from eastern (Atlantic + modified polar waters) and western (Canada-basin polar waters) Arctic sectors. The findings highlight the potential of designing in situ multi-channel DOM fluorometers to trace the freshwater origins and decipher water mass mixing dynamics in the region without laborious samples analyses. PMID:27667721

The Study on Mental Health at Work: Design and sampling.

PubMed

Rose, Uwe; Schiel, Stefan; Schröder, Helmut; Kleudgen, Martin; Tophoven, Silke; Rauch, Angela; Freude, Gabriele; Müller, Grit

2017-08-01

The Study on Mental Health at Work (S-MGA) generates the first nationwide representative survey enabling the exploration of the relationship between working conditions, mental health and functioning. This paper describes the study design, sampling procedures and data collection, and presents a summary of the sample characteristics. S-MGA is a representative study of German employees aged 31-60 years subject to social security contributions. The sample was drawn from the employment register based on a two-stage cluster sampling procedure. Firstly, 206 municipalities were randomly selected from a pool of 12,227 municipalities in Germany. Secondly, 13,590 addresses were drawn from the selected municipalities for the purpose of conducting 4500 face-to-face interviews. The questionnaire covers psychosocial working and employment conditions, measures of mental health, work ability and functioning. Data from personal interviews were combined with employment histories from register data. Descriptive statistics of socio-demographic characteristics and logistic regressions analyses were used for comparing population, gross sample and respondents. In total, 4511 face-to-face interviews were conducted. A test for sampling bias revealed that individuals in older cohorts participated more often, while individuals with an unknown educational level, residing in major cities or with a non-German ethnic background were slightly underrepresented. There is no indication of major deviations in characteristics between the basic population and the sample of respondents. Hence, S-MGA provides representative data for research on work and health, designed as a cohort study with plans to rerun the survey 5 years after the first assessment.

Proteomic analysis of the thermophilic methylotroph Bacillus methanolicus MGA3.

PubMed

Müller, Jonas E N; Litsanov, Boris; Bortfeld-Miller, Miriam; Trachsel, Christian; Grossmann, Jonas; Brautaset, Trygve; Vorholt, Julia A

2014-03-01

Bacillus methanolicus MGA3 is a facultative methylotroph of industrial relevance that is able to grow on methanol as its sole source of carbon and energy. The Gram-positive bacterium possesses a soluble NAD(+) -dependent methanol dehydrogenase and assimilates formaldehyde via the ribulose monophosphate (RuMP) cycle. We used label-free quantitative proteomics to generate reference proteome data for this bacterium and compared the proteome of B. methanolicus MGA3 on two different carbon sources (methanol and mannitol) as well as two different growth temperatures (50°C and 37°C). From a total of approximately 1200 different detected proteins, approximately 1000 of these were used for quantification. While the levels of 213 proteins were significantly different at the two growth temperatures tested, the levels of 109 proteins changed significantly when cells were grown on different carbon sources. The carbon source strongly affected the synthesis of enzymes related to carbon metabolism, and in particular, both dissimilatory and assimilatory RuMP cycle enzyme levels were elevated during growth on methanol compared to mannitol. Our data also indicate that B. methanolicus has a functional tricarboxylic acid cycle, the proteins of which are differentially regulated on mannitol and methanol. Other proteins presumed to be involved in growth on methanol were constitutively expressed under the different growth conditions. All MS data have been deposited in the ProteomeXchange with the identifiers PXD000637 and PXD000638 (http://proteomecentral.proteomexchange.org/dataset/PXD000637, http://proteomecentral.proteomexchange.org/dataset/PXD000638). © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Development and characterization of a ferroelectric non-volatile memory for flexible electronics

NASA Astrophysics Data System (ADS)

Mao, Duo

Flexible electronics have received significant attention recently because of the potential applications in displays, sensors, radio frequency identification (RFID) tags and other integrated circuits. Electrically addressable non-volatile memory is a key component for these applications. The major challenges are to fabricate the memory at a low temperature compatible with plastic substrates while maintaining good device reliability, by being compatible with process as needed to integrate with other electronic components for system-on-chip applications. In this work, ferroelectric capacitors fabricated at low temperature were developed. Based on that, a ferroelectric random access memory (FRAM) for flexible electronics was developed and characterized. Poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] copolymer was used as a ferroelectric material and a photolithographic process was developed to fabricate ferroelectric capacitors. Different characterization methods including atomic force microscopy, x-ray diffraction and Fourier-transform infrared reflection-absorption spectroscopy were used to study the material properties of the P(VDF-TrFE) film. The material properties were correlated with the electrical characteristics of the ferroelectric capacitors. To understand the polarization switching behavior of the P(VDF-TrFE) ferroelectric capacitors, a Nucleation-Limited-Switching (NLS) model was used to study the switching kinetics. The switching kinetics were characterized over the temperature range from -60 °C to 100 °C. Fatigue characteristics were studied at different electrical stress voltages and frequencies to evaluate the reliability of the ferroelectric capacitor. The degradation mechanism is attributed to the increase of the activation field and the suppression of the switchable polarization. To develop a FRAM circuit for flexible electronics, an n-channel thin film transistor (TFT) based on CdS as the semiconductor was integrated with a P(VDF-TrFE) ferroelectric capacitor for a one-transistor-one-capacitor (1T1C) memory cell. The 1T1C devices were fabricated at low temperature and demonstrated a memory window (DeltaVBL) of 2.3 V and 3.5 V, depending on the device dimensions. Next, FRAM arrays (4-bit, 16-bit and 64-bit) based on the two-transistor-two-capacitor (2T2C) memory cell architecture were designed and fabricated using a photolithographic process with 9 masks. The fabricated FRAM arrays were packaged in 28-pin ceramic packages. The read/write schemes were developed and the FRAM arrays show successful program and erase with a memory window of approximately 1 V at the output of the sense amplifier.

Remote sensing of the Fram Strait marginal ice zone

USGS Publications Warehouse

Shuchman, R.A.; Burns, B.A.; Johannessen, O.M.; Josberger, E.G.; Campbell, W.J.; Manley, T.O.; Lannelongue, N.

1987-01-01

Sequential remote sensing images of the Fram Strait marginal ice zone played a key role in elucidating the complex interactions of the atmosphere, ocean, and sea ice. Analysis of a subset of these images covering a 1-week period provided quantitative data on the mesoscale ice morphology, including ice edge positions, ice concentrations, floe size distribution, and ice kinematics. The analysis showed that, under light to moderate wind conditions, the morphology of the marginal ice zone reflects the underlying ocean circulation. High-resolution radar observations showed the location and size of ocean eddies near the ice edge. Ice kinematics from sequential radar images revealed an ocean eddy beneath the interior pack ice that was verified by in situ oceanographic measurements.

Automated trajectory planning for multiple-flyby interplanetary missions

NASA Astrophysics Data System (ADS)

Englander, Jacob

Many space mission planning problems may be formulated as hybrid optimal control problems (HOCP), i.e. problems that include both real-valued variables and categorical variables. In interplanetary trajectory design problems the categorical variables will typically specify the sequence of planets at which to perform flybys, and the real-valued variables will represent the launch date, ight times between planets, magnitudes and directions of thrust, flyby altitudes, etc. The contribution of this work is a framework for the autonomous optimization of multiple-flyby interplanetary trajectories. The trajectory design problem is converted into a HOCP with two nested loops: an "outer-loop" that finds the sequence of flybys and an "inner-loop" that optimizes the trajectory for each candidate yby sequence. The problem of choosing a sequence of flybys is posed as an integer programming problem and solved using a genetic algorithm (GA). This is an especially difficult problem to solve because GAs normally operate on a fixed-length set of decision variables. Since in interplanetary trajectory design the number of flyby maneuvers is not known a priori, it was necessary to devise a method of parameterizing the problem such that the GA can evolve a variable-length sequence of flybys. A novel "null gene" transcription was developed to meet this need. Then, for each candidate sequence of flybys, a trajectory must be found that visits each of the flyby targets and arrives at the final destination while optimizing some cost metric, such as minimizing ▵v or maximizing the final mass of the spacecraft. Three different classes of trajectory are described in this work, each of which requireda different physical model and optimization method. The choice of a trajectory model and optimization method is especially challenging because of the nature of the hybrid optimal control problem. Because the trajectory optimization problem is generated in real time by the outer-loop, the inner-loop optimization algorithm cannot require any a priori information and must always return a solution. In addition, the upper and lower bounds on each decision variable cannot be chosen a priori by the user because the user has no way to know what problem will be solved. Instead a method of choosing upper and lower bounds via a set of simple rules was developed and used for all three types of trajectory optimization problem. Many optimization algorithms were tested and discarded until suitable algorithms were found for each type of trajectory. The first class of trajectories use chemical propulsion and may only apply a ▵v at the periapse of each flyby. These Multiple Gravity Assist (MGA) trajectories are optimized using a cooperative algorithm of Differential Evolution (DE) and Particle Swarm Optimization (PSO). The second class of trajectories, known as Multiple Gravity Assist with one Deep Space Maneuver (MGA-DSM), also use chemical propulsion but instead of maneuvering at the periapse of each flyby as in the MGA case a maneuver is applied at a free point along each planet-to-planet arc, i.e. there is one maneuver for each pair of flybys. MGA-DSM trajectories are parameterized by more variables than MGA trajectories, and so the cooperative algorithm of DE and PSO that was used to optimize MGA trajectories was found to be less effective when applied to MGA-DSM. Instead, either PSO or DE alone were found to be more effective. The third class of trajectories addressed in this work are those using continuousthrust propulsion. Continuous-thrust trajectory optimization problems are more challenging than impulsive-thrust problems because the control variables are a continuous time series rather than a small set of parameters and because the spacecraft does not follow a conic section trajectory, leading to a large number of nonlinear constraints that must be satisfied to ensure that the spacecraft obeys the equations of motion. Many models and optimization algorithms were applied including direct transcription with nonlinear programming (DTNLP), the inverse-polynomial shapebased method, and feasible region analysis. However the only physical model and optimization method that proved reliable enough were the Sims-Flanagan transcription coupled with a nonlinear programming solver and the monotonic basin hopping (MBH) global search heuristic. The methods developed here are demonstrated to optimize a set of example trajectories, including a recreation of the Cassini mission, a Galileo-like mission, and conceptual continuous-thrust missions to Jupiter, Mercury, and Uranus.

Flash-Type Discrimination

NASA Technical Reports Server (NTRS)

Koshak, William J.

2010-01-01

This viewgraph presentation describes the significant progress made in the flash-type discrimination algorithm development. The contents include: 1) Highlights of Progress for GLM-R3 Flash-Type discrimination Algorithm Development; 2) Maximum Group Area (MGA) Data; 3) Retrieval Errors from Simulations; and 4) Preliminary Global-scale Retrieval.

Atlantic water heat transfer through the Arctic Gateway (Fram Strait) during the Last Interglacial

NASA Astrophysics Data System (ADS)

Zhuravleva, Anastasia; Bauch, Henning A.; Spielhagen, Robert F.

2017-10-01

The Last Interglacial in the Arctic region is often described as a time with warmer conditions and significantly less summer sea ice than today. The role of Atlantic water (AW) as the main oceanic heat flux agent into the Arctic Ocean remains, however, unclear. Using high-resolution stable isotope and faunal records from the only deep Arctic Gateway, the Fram Strait, we note for the upper water column a diminished influence of AW and generally colder-than-Holocene surface ocean conditions. After the main Saalian deglaciation had terminated, a first intensification of northward-advected AW happened ( 124 ka). However, an intermittent sea surface cooling, triggered by meltwater release at 122 ka, caused a regional delay in the further development towards peak interglacial conditions. Maximum AW heat advection occurred during late MIS 5e (118.5-116 ka) and interrupted a longer-term cooling trend at the sea surface that started from about 120 ka on. Such a late occurrence of the major AW-derived near-surface warming in the Fram Strait - this is in stark contrast to an early warm peak in the Holocene - compares well in time with upstream records from the Norwegian Sea, altogether implying a coherent development of south-to-north ocean heat transfer through the eastern Nordic Seas and into the high Arctic during the Last Interglacial.

Structure-based Mechanism of CMP-2-keto-3-deoxymanno-octulonic Acid Synthetase

PubMed Central

Heyes, Derren J.; Levy, Colin; Lafite, Pierre; Roberts, Ian S.; Goldrick, Marie; Stachulski, Andrew V.; Rossington, Steven B.; Stanford, Deborah; Rigby, Stephen E. J.; Scrutton, Nigel S.; Leys, David

2009-01-01

The enzyme CMP-Kdo synthetase (KdsB) catalyzes the addition of 2-keto-3-deoxymanno-octulonic acid (Kdo) to CTP to form CMP-Kdo, a key reaction in the biosynthesis of lipopolysaccharide. The reaction catalyzed by KdsB and the related CMP-acylneuraminate synthase is unique among the sugar-activating enzymes in that the respective sugars are directly coupled to a cytosine monophosphate. Using inhibition studies, in combination with isothermal calorimetry, we show the substrate analogue 2β-deoxy-Kdo to be a potent competitive inhibitor. The ligand-free Escherichia coli KdsB and ternary complex KdsB-CTP-2β-deoxy-Kdo crystal structures reveal that Kdo binding leads to active site closure and repositioning of the CTP phosphates and associated Mg2+ ion (Mg-B). Both ligands occupy conformations compatible with an Sn2-type attack on the α-phosphate by the Kdo 2-hydroxyl group. Based on strong similarity with DNA/RNA polymerases, both in terms of overall chemistry catalyzed as well as active site configuration, we postulate a second Mg2+ ion (Mg-A) is bound by the catalytically competent KdsB-CTP-Kdo ternary complex. Modeling of this complex reveals the Mg-A coordinated to the conserved Asp100 and Asp235 in addition to the CTP α-phosphate and both the Kdo carboxylic and 2-hydroxyl groups. EPR measurements on the Mn2+-substituted ternary complex support this model. We propose the KdsB/CNS sugar-activating enzymes catalyze the formation of activated sugars, such as the abundant CMP-5-N-acetylneuraminic acid, by recruitment of two Mg2+ to the active site. Although each metal ion assists in correct positioning of the substrates and activation of the α-phosphate, Mg-A is responsible for activation of the sugar-hydroxyl group. PMID:19815542

Restoration of reproductive potential after expiration or removal of melengestrol acetate contraceptive implants in Tigers (Panthera tigris).

PubMed

Chuei, Jason Y; Asa, Cheryl S; Hall-Woods, Monica; Ballou, Jonathon; Traylor-Holzer, Kathy

2007-07-01

The need for contraception in the successful management of captive wild animals is becoming increasingly apparent. Because concerns exist regarding the reversibility of the contraceptive implant melengestrol acetate (MGA), reproductive data for 94 female Amur (Panthera tigris altaica) and Sumatran tigers (Panthera tigris sumatrae) were analyzed using survival analyses to evaluate return to reproductive status after implant removal or assumed expiration. Females placed in potential breeding situations after MGA implants were surgically removed showed a 62% return to reproduction by 5.25 years, whereas females with implants that were assumed to have expired showed only a 30% return to reproduction by 6 years. Implanted females did not reproduce as successfully as non-implanted control females, which showed an 85% probability of reproducing after placement in a new breeding situation by 2.66 years. Parturition increased the probability of reproducing in non-implanted females, but not in implanted females. Litter size, stillbirths, and offspring survival were not significantly different between non-implanted, implant-removed and implant-expired female tigers. Ten female tigers reproduced both before and after implant placement, and the differences in litter size, stillbirths, and offspring survival were not significant, nor were they significantly different from non-implanted females. Prior parturition, age when implant was removed, and duration of implantation did not affect the probability of reproducing for females after implant removal. These results show substantial reversibility of MGA implants, leading to 62% successful reproduction after implant removal. The reasons for lower successful reproduction in animals previously treated with the contraceptive compared to non-implanted females are not known, but a greater delay in reversibility was seen when implants were left in place and only presumed expired. Zoo Biol 26:275-288, 2007. (c) 2007 Wiley-Liss, Inc.

MYB Labeling by Immunohistochemistry Is More Sensitive and Specific for Breast Adenoid Cystic Carcinoma than MYB Labeling by FISH.

PubMed

Poling, Justin S; Yonescu, Raluca; Subhawong, Andrea P; Sharma, Rajni; Argani, Pedram; Ning, Yi; Cimino-Mathews, Ashley

2017-07-01

Breast adenoid cystic carcinoma (ACC) is a primary breast carcinoma that, like salivary gland ACC, displays the t(6;9) translocation resulting in the MYB-NFIB gene fusion and immunopositivity for MYB by immunohistochemistry (IHC). However, it is not well established whether MYB immunoreactivity or rearrangement can be used to support a diagnosis of ACC in a malignant basaloid or benign cribriform breast lesion. Whole sections of primary breast ACC (n=11), collagenous spherulosis (CS; n=7), and microglandular adenosis (MGA; n=5) and tissue microarrays containing 16 basal-like, triple-negative breast carcinomas (TNBC) were labeled for MYB by IHC and underwent MYB fluorescence in situ hybridization using a break-apart probe. Strong, diffuse nuclear MYB labeling was seen in 100% ACC compared with no cases of basal-like TNBC, CS, or MGA (P=0.0001). Any degree of nuclear MYB labeling was seen in 100% ACC compared with 54% of all other cases (P=0.007), with any labeling seen in 71% CS, 63% basal-like TNBC, and 0% MGA. MYB rearrangement was detected in 89% (8/9) of evaluable ACC compared with 4% (1/26) of all other evaluable cases (P=0.0001), with a rearrangement detected in 1 (7%; n=1/15) evaluable basal-like TNBC. Strong, diffuse nuclear labeling for MYB is more sensitive than MYB fluorescence in situ hybridization for breast ACC and can be used to support a diagnosis of ACC in a cribriform or basaloid lesion in the breast. However, weak and focal labeling should be interpreted with caution as it can be seen in other benign cribriform and malignant basaloid lesions.

Optics of Water Microdroplets with Soot Inclusions: Exact Versus Approximate Results

NASA Technical Reports Server (NTRS)

Liu, Li; Mishchenko, Michael I.

2016-01-01

We use the recently generalized version of the multi-sphere superposition T-matrix method (STMM) to compute the scattering and absorption properties of microscopic water droplets contaminated by black carbon. The soot material is assumed to be randomly distributed throughout the droplet interior in the form of numerous small spherical inclusions. Our numerically-exact STMM results are compared with approximate ones obtained using the Maxwell-Garnett effective-medium approximation (MGA) and the Monte Carlo ray-tracing approximation (MCRTA). We show that the popular MGA can be used to calculate the droplet optical cross sections, single-scattering albedo, and asymmetry parameter provided that the soot inclusions are quasi-uniformly distributed throughout the droplet interior, but can fail in computations of the elements of the scattering matrix depending on the volume fraction of soot inclusions. The integral radiative characteristics computed with the MCRTA can deviate more significantly from their exact STMM counterparts, while accurate MCRTA computations of the phase function require droplet size parameters substantially exceeding 60.

A comparison of radiation budgets in the Fram Strait marginal ice zone

NASA Technical Reports Server (NTRS)

Francis, Jennifer A.; Katsaros, Kristina B.; Ackerman, Thomas P.; Lind, Richard J.; Davidson, Kenneth L.

1991-01-01

Results are presented from calculations of radiation budgets for the sea-ice and the open-water regimes in the marginal ice zone (MIZ) of the Fram Strait, from measurements of surface irradiances and meteorological conditions made during the 1984 Marginal Ice Zone Experiment. Simultaneous measurements on either side of the ice edge allowed a comparison of the open-water and the sea-ice environments. The results show significant differences between the radiation budgets of the two regimes in the MIZ. The open water absorbed twice as much radiation as did the ice, and the mean cooling rate of the atmosphere over water was approximately 15 percent larger than that over ice. Calculated fluxes and atmospheric cooling rates were found to compare well with available literature data.

FRAM-2012: Norwegians return to the High Arctic with a Hovercraft for Marine Geophysical Research

NASA Astrophysics Data System (ADS)

Hall, J. K.; Kristoffersen, Y.; Brekke, H.; Hope, G.

2012-12-01

After four years of testing methods, craft reliability, and innovative equipment, the R/H SABVABAA has embarked on its first FRAM-201x expedition to the highest Arctic. Named after the Inupiaq word for 'flows swiftly over it', the 12m by 6m hovercraft has been home-based in Longyearbyen, Svalbard since June 2008. In this, its fifth summer of work on the ice pack north of 81N, the craft is supported by the Norwegian Petroleum Directorate (NPD) via the Nansen Environmental and Remote Sensing Center (NERSC) in Bergen, and the Norwegian Scientific Academy for Polar Research. FRAM-2012 represents renewed Norwegian interest in returning to the highest Arctic some 116 years after the 1893-96 drift of Fridtjof Nansen's ship FRAM, the first serious scientific investigation of the Arctic. When replenished by air or icebreaker, the hovercraft Sabvabaa offers a hospitable scientific platform with crew of two, capable of marine geophysical, geological and oceanographic observations over long periods with relative mobility on the ice pack. FRAM-2012 is the first step towards this goal, accompanying the Swedish icebreaker ODEN to the Lomonosov Ridge, north of Greenland, as part of the LOMROG III expedition. The science plan called for an initial drive from the ice edge to Gakkel Ridge at 85N where micro-earthquakes would be monitored, and then to continue north to a geological sampling area on the Lomonosov Ridge at about 88N, 65W. The micro-earthquake monitoring is part of Gaute Hope's MSc thesis and entails five hydrophones in a WiFi-connected hydrophone array deployed over the Gakkel Rift Valley, drifting with the ice at up to 0.4 knots. On August 3 the hovercraft was refueled from icebreaker ODEN at 84-21'N and both vessels proceeded north. The progress of the hovercraft was hampered by insufficient visibility for safe driving and time consuming maneuvering in and around larger fields of rubble ice impassable by the hovercraft, but of little concern to the icebreaker. It became clear that to compensate for delayed rendezvous would take up substantially more icebreaker time than initially agreed to. It was therefore decided that the hovercraft would remain in the Gakkel Ridge survey area while the icebreaker would concentrate on its primary mission objective, a Danish UNCLOS survey. The two vessels would rejoin for the return journey to Svalbard in early September. The hovercraft has made continuous ice thickness measurements along its track by a front-mounted electromagnetic survey instrument in combination with a sonic height measuring device. The poster will present the findings of the expedition, and a short video is under preparation.

Variability in pteropod sedimentation and corresponding aragonite flux at the Arctic deep-sea long-term observatory HAUSGARTEN in the eastern Fram Strait from 2000 to 2009

NASA Astrophysics Data System (ADS)

Bauerfeind, E.; Nöthig, E.-M.; Pauls, B.; Kraft, A.; Beszczynska-Möller, A.

2014-04-01

Pteropods are an important component of the zooplankton community and hence of the food web in the Fram Strait. They have a calcareous (aragonite) shell and are thus sensitive in particular to the effects of the increasing CO2 concentration in the atmosphere and the associated changes of pH and temperature in the ocean. In the eastern Fram Strait, two species of thecosome pteropods occur, the cold water-adapted Limacina helicina and the subarctic boreal species Limacina retroversa. Both species were regularly observed in year-round moored sediment traps at ~ 200-300 m depth in the deep-sea long-term observatory HAUSGARTEN (79°N, 4°E). The flux of all pteropods found in the trap samples varied from < 20 to ~ 870 specimen m- 2 d- 1 in the years 2000-2009, being lower during the period 2000-2006. At the beginning of the time series, pteropods were dominated by the cold-water-adapted L. helicina, whereas the subarctic boreal L. retroversa was only occasionally found in large quantities (> 50 m- 2 d- 1). This picture completely changed after 2005/6 when L. retroversa became dominant and total pteropod numbers in the trap samples increased significantly. Concomitant to this shift in species composition, a warming event occurred in 2005/6 and persisted until the end of the study in 2009, despite a slight cooling in the upper water layer after 2007/8. Sedimentation of pteropods showed a strong seasonality, with elevated fluxes of L. helicina from August to November. Numbers of L. retroversa usually increased later, during September/October, with a maximum at the end of the season during December/January. In terms of carbonate export, aragonite shells of pteropods contributed with 11-77% to the annual total CaCO3 flux in Fram Strait. The highest share was found in the period 2007 to 2009, predominantly during sedimentation events at the end of the year. Results obtained by sediment traps occasionally installed on a benthic lander revealed that pteropods also arrive at the seafloor (~ 2550 m) almost simultaneous with their occurrence in the shallower traps. This indicates a rapid downward transport of calcareous shells, which provides food particles for the deep-sea benthos during winter when other production in the upper water column is shut down. The results of our study highlight the great importance of pteropods for the biological carbon pump as well as for the carbonate system in Fram Strait at present, and indicate modifications within the zooplankton community. The results further emphasize the importance of long-term investigation to disclose such changes.

Innovations in the Assay of Un-Segregated Multi-Isotopic Grade TRU Waste Boxes with SuperHENC and FRAM Technology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Simpson, A. P.; Barber, S.; Abdurrahman, N. M.

2006-07-01

The Super High Efficiency Neutron Coincidence Counter (SuperHENC) was originally developed by BIL Solutions Inc., Los Alamos National Laboratory (LANL) and Rocky Flats Environmental Technology Site (RFETS) for assay of transuranic (TRU) waste in Standard Waste Boxes (SWB) at Rocky Flats. This mobile system was a key component in the shipment of over 4,000 SWBs to the Waste Isolation Pilot Plant (WIPP) in Carlsbad, New Mexico. The system was WIPP certified in 2001 and operated at the site for four years. The success of this system, a passive neutron coincidence counter combined with high resolution gamma spectroscopy, led to themore » order of two new units, delivered to Hanford in 2004. Several new challenges were faced at Hanford: For example, the original RFETS system was calibrated for segregated waste streams such that metals, plastics, wet combustibles and dry combustibles were separated by 'Item Description Codes' prior to assay. Furthermore, the RFETS mission of handling only weapons grade plutonium, enabled the original SuperHENC to benefit from the use of known Pu isotopics. Operations at Hanford, as with most other DOE sites, generate un-segregated waste streams, with a wide diversity of Pu isotopics. Consequently, the new SuperHENCs are required to deal with new technical challenges. The neutron system's software and calibration methodology have been modified to encompass these new requirements. In addition, PC-FRAM software has been added to the gamma system, providing a robust isotopic measurement capability. Finally a new software package has been developed that integrates the neutron and gamma data to provide a final assay results and analysis report. The new system's performance has been rigorously tested and validated against WIPP quality requirements. These modifications, together with the mobile platform, make the new SuperHENC far more versatile in handling diverse waste streams and allow for rapid redeployment around the DOE complex. (authors)« less

Chimeric Antigen Receptors to CD276 for Treating Cancer | NCI Technology Transfer Center | TTC

Cancer.gov

This licensing opportunity from the National Cancer Institute concerns the development of CARs comprising an antigen-binding fragment derived from the MGA271 antibody. The resulting CARs can be used in adoptive cell therapy treatment for neuroblastoma and other tumors that express CD276.

Dissolved Organophosphate Esters and Polybrominated Diphenyl Ethers in Remote Marine Environments: Arctic Surface Water Distributions and Net Transport through Fram Strait.

PubMed

McDonough, Carrie A; De Silva, Amila O; Sun, Caoxin; Cabrerizo, Ana; Adelman, David; Soltwedel, Thomas; Bauerfeind, Eduard; Muir, Derek C G; Lohmann, Rainer

2018-06-05

Organophosphate esters (OPEs) have been found in remote environments at unexpectedly high concentrations, but very few measurements of OPE concentrations in seawater are available, and none are available in subsurface seawater. In this study, passive polyethylene samplers (PEs) deployed on deep-water moorings in the Fram Strait and in surface waters of Canadian Arctic lakes and coastal sites were analyzed for a suite of common OPEs. Total OPEs ( ∑ 11 OPE) at deep-water sites were dominated by chlorinated OPEs, and ranged from 6.3 to 440 pg/L. Concentrations were similar in eastern and western Fram Strait. Chlorinated OPEs were also dominant in Canadian Arctic surface waters (mean concentration ranged from < DL to 4400 pg/L), while nonhalogenated alkyl/aryl-substituted OPEs remained low (1.3-55 pg/L), possibly due to the greater long-range transport potential of chlorinated OPEs. Polybrominated diphenyl ethers (PBDEs) were found at much lower concentrations than OPEs (

Short-Term Plasticity of the Visuomotor Map during Grasping Movements in Humans

ERIC Educational Resources Information Center

Safstrom, Daniel; Edin, Benoni B.

2005-01-01

During visually guided grasping movements, visual information is transformed into motor commands. This transformation is known as the "visuomotor map." To investigate limitations in the short-term plasticity of the visuomotor map in normal humans, we studied the maximum grip aperture (MGA) during the reaching phase while subjects grasped objects…

Multiobjective genetic algorithm conjunctive use optimization for production, cost, and energy with dynamic return flow

NASA Astrophysics Data System (ADS)

Peralta, Richard C.; Forghani, Ali; Fayad, Hala

2014-04-01

Many real water resources optimization problems involve conflicting objectives for which the main goal is to find a set of optimal solutions on, or near to the Pareto front. E-constraint and weighting multiobjective optimization techniques have shortcomings, especially as the number of objectives increases. Multiobjective Genetic Algorithms (MGA) have been previously proposed to overcome these difficulties. Here, an MGA derives a set of optimal solutions for multiobjective multiuser conjunctive use of reservoir, stream, and (un)confined groundwater resources. The proposed methodology is applied to a hydraulically and economically nonlinear system in which all significant flows, including stream-aquifer-reservoir-diversion-return flow interactions, are simulated and optimized simultaneously for multiple periods. Neural networks represent constrained state variables. The addressed objectives that can be optimized simultaneously in the coupled simulation-optimization model are: (1) maximizing water provided from sources, (2) maximizing hydropower production, and (3) minimizing operation costs of transporting water from sources to destinations. Results show the efficiency of multiobjective genetic algorithms for generating Pareto optimal sets for complex nonlinear multiobjective optimization problems.

Demographic patterns of postfire regeneration in mediterranean-climate shrublands of California

USGS Publications Warehouse

Keeley, J.E.; Fotheringham, C.J.; Baer-Keeley, M.

2006-01-01

Eggs of captive black ducks fed diets containing DDE at 10 and 30 ppm (dry weight) experienced significant shell thinning and an increase in shell cracking when compared to eggs of untreated black ducks. Eggshells from dosed ducks were: 18-24 percent thinner at the equator than shells from undosed ducks; 28-31 percent thinner at the cap; and 29-38 percent thinner at the apex. Shell cracking averaged 21 percent among eggs fram the 30 ppm DDE dosage and 10 percent among eggs from the 10 ppm dosage. Only 2 percent of the eggs from untreated black ducks were cracked. Survival of ducklings fram dosed parents in terms of 'percentage of 21-day ducklings of embryonated eggs' was 40-76 percent lower than survival of ducklings from undosed parents. Average DDE residues (wet weight) in eggs from hens fed 10 and 30 ppm DDE were 46 ppm and 144 ppm, respectively.

Models for Total-Dose Radiation Effects in Non-Volatile Memory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Campbell, Philip Montgomery; Wix, Steven D.

The objective of this work is to develop models to predict radiation effects in non- volatile memory: flash memory and ferroelectric RAM. In flash memory experiments have found that the internal high-voltage generators (charge pumps) are the most sensitive to radiation damage. Models are presented for radiation effects in charge pumps that demonstrate the experimental results. Floating gate models are developed for the memory cell in two types of flash memory devices by Intel and Samsung. These models utilize Fowler-Nordheim tunneling and hot electron injection to charge and erase the floating gate. Erase times are calculated from the models andmore » compared with experimental results for different radiation doses. FRAM is less sensitive to radiation than flash memory, but measurements show that above 100 Krad FRAM suffers from a large increase in leakage current. A model for this effect is developed which compares closely with the measurements.« less

Ice Sheet and Sea Ice Observations from Unmanned Aircraft Systems

NASA Astrophysics Data System (ADS)

Crocker, R. I.; Maslanik, J. A.

2011-12-01

A suite of sensors has been assembled to map ice sheet and sea ice surface topography with fine-resolution from small unmanned aircraft systems (UAS). This payload is optimized to provide coincident surface elevation and imagery data, and with its low cost and ease of reproduction, it has the potential to become a widely-distributed observational resource to complement polar manned-aircraft and satellite missions. To date, it has been deployed to map ice sheet elevations near Jakobshavn Isbræ in Greenland, and to measure sea ice freeboard and roughness in Fram Strait off the coast of Svalbard. Data collected during these campaigns have facilitate a detailed assessment of the system's surface elevation measurement accuracy, and provide a glimpse of the summer 2009 Fram Strait sea ice conditions. These findings are presented, along with a brief overview of our future Arctic UAS operations.

The Ground Flash Fraction Retrieval Algorithm Employing Differential Evolution: Simulations and Applications

NASA Technical Reports Server (NTRS)

Koshak, William; Solakiewicz, Richard

2012-01-01

The ability to estimate the fraction of ground flashes in a set of flashes observed by a satellite lightning imager, such as the future GOES-R Geostationary Lightning Mapper (GLM), would likely improve operational and scientific applications (e.g., severe weather warnings, lightning nitrogen oxides studies, and global electric circuit analyses). A Bayesian inversion method, called the Ground Flash Fraction Retrieval Algorithm (GoFFRA), was recently developed for estimating the ground flash fraction. The method uses a constrained mixed exponential distribution model to describe a particular lightning optical measurement called the Maximum Group Area (MGA). To obtain the optimum model parameters (one of which is the desired ground flash fraction), a scalar function must be minimized. This minimization is difficult because of two problems: (1) Label Switching (LS), and (2) Parameter Identity Theft (PIT). The LS problem is well known in the literature on mixed exponential distributions, and the PIT problem was discovered in this study. Each problem occurs when one allows the numerical minimizer to freely roam through the parameter search space; this allows certain solution parameters to interchange roles which leads to fundamental ambiguities, and solution error. A major accomplishment of this study is that we have employed a state-of-the-art genetic-based global optimization algorithm called Differential Evolution (DE) that constrains the parameter search in such a way as to remove both the LS and PIT problems. To test the performance of the GoFFRA when DE is employed, we applied it to analyze simulated MGA datasets that we generated from known mixed exponential distributions. Moreover, we evaluated the GoFFRA/DE method by applying it to analyze actual MGAs derived from low-Earth orbiting lightning imaging sensor data; the actual MGA data were classified as either ground or cloud flash MGAs using National Lightning Detection Network[TM] (NLDN) data. Solution error plots are provided for both the simulations and actual data analyses.

Growth of Bacillus methanolicus in 2 M methanol at 50 °C: the effect of high methanol concentration on gene regulation of enzymes involved in formaldehyde detoxification by the ribulose monophosphate pathway.

PubMed

Bozdag, Ahmet; Komives, Claire; Flickinger, Michael C

2015-07-01

Bacillus methanolicus MGA3 is a Gram-positive aerobic methylotroph growing optimally at 50-53°C. Methylotrophy in B. methanolicus is encoded on pBM19 and by two chromosomal copies of the methanol dehydrogenase (mdh), hexulose phosphate synthase (hps) and phosphohexuloisomerase (phi) genes. However, there are no published studies on the regulation of methylotrophy or the dominant mechanism of detoxification of intracellular formaldehyde in response to high methanol concentration. The µ max of B. methanolicus MGA3 was assessed on methanol, mannitol and glucose. B. methanolicus achieved a µ max at 25 mM initial methanol of 0.65 ± 0.007 h(-1), which decreased to 0.231 ± 0.004 h(-1) at 2 M initial methanol. Slow growth was also observed with initial methanol concentrations of >2 M. The µ max on mannitol and glucose are 0.532 ± 0.002 and 0.336 ± 0.003 h(-1), respectively. Spiking cultures with additional methanol (100 mM) did not disturb the growth rate of methanol-grown cells, whereas, a 50 mM methanol spike halted the growth in mannitol. Surprisingly, growth in methanol was inhibited by 1 mM formaldehyde, while mannitol-grown cells tolerated 2 mM. Moreover, mannitol-grown cells removed formaldehyde faster than methanol-grown cells. Further, we show that methanol oxidation in B. methanolicus MGA3 is mainly carried out by the pBM19-encoded mdh. Formaldehyde and formate addition down-regulate the mdh and hps genes in methanol-grown cells. Similarly, they down-regulate mdh genes in mannitol-grown cells, but up-regulate hps. Phosphofructokinase (pfk) is up-regulated in both methanol and mannitol-grown cells, which suggests that pfk may be a possible synthetic methylotrophy target to reduce formaldehyde growth toxicity at high methanol concentrations.

The large-scale freshwater cycle of the Arctic

NASA Astrophysics Data System (ADS)

Serreze, Mark C.; Barrett, Andrew P.; Slater, Andrew G.; Woodgate, Rebecca A.; Aagaard, Knut; Lammers, Richard B.; Steele, Michael; Moritz, Richard; Meredith, Michael; Lee, Craig M.

2006-11-01

This paper synthesizes our understanding of the Arctic's large-scale freshwater cycle. It combines terrestrial and oceanic observations with insights gained from the ERA-40 reanalysis and land surface and ice-ocean models. Annual mean freshwater input to the Arctic Ocean is dominated by river discharge (38%), inflow through Bering Strait (30%), and net precipitation (24%). Total freshwater export from the Arctic Ocean to the North Atlantic is dominated by transports through the Canadian Arctic Archipelago (35%) and via Fram Strait as liquid (26%) and sea ice (25%). All terms are computed relative to a reference salinity of 34.8. Compared to earlier estimates, our budget features larger import of freshwater through Bering Strait and larger liquid phase export through Fram Strait. While there is no reason to expect a steady state, error analysis indicates that the difference between annual mean oceanic inflows and outflows (˜8% of the total inflow) is indistinguishable from zero. Freshwater in the Arctic Ocean has a mean residence time of about a decade. This is understood in that annual freshwater input, while large (˜8500 km3), is an order of magnitude smaller than oceanic freshwater storage of ˜84,000 km3. Freshwater in the atmosphere, as water vapor, has a residence time of about a week. Seasonality in Arctic Ocean freshwater storage is nevertheless highly uncertain, reflecting both sparse hydrographic data and insufficient information on sea ice volume. Uncertainties mask seasonal storage changes forced by freshwater fluxes. Of flux terms with sufficient data for analysis, Fram Strait ice outflow shows the largest interannual variability.

Arctic Ocean outflow and glacier-ocean interactions modify water over the Wandel Sea shelf (northeastern Greenland)

NASA Astrophysics Data System (ADS)

Dmitrenko, Igor A.; Kirillov, Sergey A.; Rudels, Bert; Babb, David G.; Toudal Pedersen, Leif; Rysgaard, Søren; Kristoffersen, Yngve; Barber, David G.

2017-12-01

The first-ever conductivity-temperature-depth (CTD) observations on the Wandel Sea shelf in northeastern Greenland were collected in April-May 2015. They were complemented by CTDs taken along the continental slope during the Norwegian FRAM 2014-2015 drift. The CTD profiles are used to reveal the origin of water masses and interactions with ambient water from the continental slope and the tidewater glacier outlet. The subsurface water is associated with the Pacific water outflow from the Arctic Ocean. The underlying halocline separates the Pacific water from a deeper layer of polar water that has interacted with the warm Atlantic water outflow through the Fram Strait, recorded below 140 m. Over the outer shelf, the halocline shows numerous cold density-compensated intrusions indicating lateral interaction with an ambient polar water mass across the continental slope. At the front of the tidewater glacier outlet, colder and turbid water intrusions were observed at the base of the halocline. On the temperature-salinity plots these stations indicate a mixing line that is different from the ambient water and seems to be conditioned by the ocean-glacier interaction. Our observations of Pacific water are set within the context of upstream observations in the Beaufort Sea and downstream observations from the Northeast Water Polynya, and clearly show the modification of Pacific water during its advection across the Arctic Ocean. Moreover, ambient water over the Wandel Sea slope shows different thermohaline structures indicating the different origin and pathways of the on-shore and off-shore branches of the Arctic Ocean outflow through the western Fram Strait.

Randomized, allopurinol-controlled trial of the effects of dietary nucleotides and active hexose correlated compound in the treatment of canine leishmaniosis.

PubMed

Segarra, Sergi; Miró, Guadalupe; Montoya, Ana; Pardo-Marín, Luis; Boqué, Noemí; Ferrer, Lluis; Cerón, José

2017-05-30

First-line treatment for canine leishmaniosis (CanL) is N-methylglucamine antimoniate (MGA) combined with allopurinol. However, in some dogs allopurinol may induce hyperxanthinuria leading to urolithiasis. Moreover, allopurinol resistance has recently been described in Leishmania infantum isolates from treated dogs with a relapse of the disease. Alternative treatments are thus needed. Since the type of host immune response strongly influences CanL progression and prognosis, dogs could benefit from treatments targeted at modulating such response, such as nucleotides and active hexose correlated compound (AHCC). The aim of this study was to evaluate the effects of an oral combination of nucleotides and AHCC in dogs with clinical leishmaniosis. Sixty-nine dogs with naturally-occurring clinical leishmaniosis were included in this multicenter, open-label, positively-controlled clinical trial and randomized to receive 10mg/kg allopurinol PO BID (allopurinol group) or 17mg/kg AHCC plus 32mg/kg nucleotides PO SID (supplement group) for 180 days. All dogs were also given 50mg/kg MGA SC BID during the first 28 days. At the time points 0, 30, and 180 days of the trial, dogs underwent a clinical examination, and blood, urine, and bone marrow samples were submitted for analytical tests. Final data analyses (allopurinol group: n=29; supplement group: n=24) revealed a significant improvement in both groups in clinical scores and ELISA-determined antibody titers after treatment. However, the supplement group showed a significantly lower clinical score (P=0.005) and significantly higher antibody titers (P=0.032) after 180 days, compared to the allopurinol group. RT-PCR parasite loads were reduced in groups (mean±SD supplement: 0.38±0.56 vs 5.23±18.9; allopurinol: 0.45±1.47 vs 3.09±8.36 parasites/ng of DNA), but there were no significant differences over time or between groups. During the study, 12 dogs in the allopurinol group developed xanthinuria (41%) compared to no dogs (0%) in the supplement group (P=0.000). Both treatments led to significantly increased CD4+/CD8+ ratio, and improvements in protein electrophoretic pattern and acute phase response. In conclusion, 6-month oral treatment with nucleotides and AHCC in addition to MGA showed similar efficacy to the current first-line treatment for CanL, without producing xanthinuria. This combination could be a good alternative to MGA-allopurinol combination treatment for CanL, especially for dogs suffering allopurinol-related adverse events. Copyright © 2017 The Author(s). Published by Elsevier B.V. All rights reserved.

Sea ice studies in the Spitsbergen-Greenland area

NASA Technical Reports Server (NTRS)

Vinje, T. E. (Principal Investigator)

1976-01-01

The author has identified the following significant results. Data showed unexpected great variations in the drift velocity of the ice in the Fram Strait. Land map improvements were achieved by LANDSAT in the eastern part of the Svalbard archipelago.

Granulosa cells from bovine follicles activate different signal transduction pathways dependent on follicle health status and ability to convert androstenedione to estrogen

USDA-ARS?s Scientific Manuscript database

Since steroidogenesis is a critical component in the development of competent preovulatory follicles we hypothesized that granulosa cells from follicles of cows treated with normal levels of progesterone (CIDR) or with melengestrol acetate (MGA), which results in the development of persistent follic...

A MODELLING FRAMEWORK FOR MERCURY CYCLING IN LAKE MICHIGAN

EPA Science Inventory

A time-dependent mercury model was developed to describe mercury cycling in Lake Michigan. The model addresses dynamic relationships between net mercury loadings and the resulting concentrations of mercury species in the water and sediment. The simplified predictive modeling fram...

30 years of Arctic sea ice thickness measurements by Royal Navy submarines

NASA Astrophysics Data System (ADS)

Wadhams, P.; Hughes, N.; Rodrigues, J. M.; Toberg, N.

2009-12-01

Royal Navy submarines fitted with upward-looking sonars have been collecting sea ice thickness data in the Arctic Ocean since the early 1970s. These data sets provide unique information on the Arctic sea ice thickness distribution and the way it has been changing in the past decades. In March 2007 HMS Tireless conducted a transect of the Arctic Ocean from Fram Strait to the western Beaufort Sea which gave the opportunity to measure the thickness of the sea ice cover during the winter immediately preceding the exceptional retreat of summer 2007. Three years earlier, in April 2004, a voyage by the same submarine took sea ice thickness data in the regions of Fram Strait, the Lincoln Sea and the North Pole. We report on the ice draft, pressure ridge and lead distributions obtained in these two cruises and analyse the evolution of the ice cover from 2004 to 2007 in areas of coincident tracks. In the region from north of Fram Strait to Ellesmere Island (about 85°N, 0-70°W) we find no change in mean drafts between 2004 and 2007 although there is a change in ice composition, with more ridging in 2007 and a slight reduction of modal draft. This agrees with the concept of young ice being driven towards Fram Strait. The region north of Ellesmere Island continues to be a "redoubt" of very thick deformed multiyear ice. In 2007 the submarine profiled extensively under the DAMOCLES ice camp at about 85°N 64°W and under the SEDNA ice camp at about 73°N 145°W. The latter is in the same location as the 1976 AIDJEX ice camp and a sonar survey done by a US submarine in April 1976. We found that a large decrease in mean draft had occurred (32%) over 31 years and that in 2007 the SEDNA region contained the thinnest ice of any part of the Arctic surveyed by the submarine. Under the DAMOCLES ice camp about 200km of topographic sea ice data were gathered with a Kongsberg EM3002 multibeam (MB) sonar, making this the largest continuous data set of its kind. The MB data produce high resolution three-dimensional images of the sea ice underside allowing for rapid demarcation of first and multi-year ice regimes along with pressure ridge classification and orientation. In order to estimate the rate of thinning of the Arctic sea ice we compare the ice thickness distributions of 2004 and 2007 with those derived from similar types of sonars that have been fitted to UK submarines on cruises since 1976. Of these, ice draft data obtained during a cruise in April 1991, and re-processing to the same standard as 2004 and 2007, has special significance because of the vast amount of data collected in Fram Strait, on the way to the Pole along the prime meridian and a survey of a region of the Arctic Ocean north of Svalbard and Franz Joseph Land.

The freshwater export from the Arctic Ocean and the circulation of liquid freshwater around Greenland - constraints, interactions & consequences

NASA Astrophysics Data System (ADS)

Rudels, Bert

2010-05-01

The freshwater added to the Arctic Ocean is stored as sea ice and as liquid freshwater residing primarily in the upper layers. This allows for simple zero order estimates of the liquid freshwater content and export based on rotationally controlled baroclinic flow. At present the freshwater outflow occurs on both sides of Greenland. In Fram Strait the sea ice export in the East Greenland Current is significantly larger than the liquid freshwater outflow, while the liquid freshwater export dominates in the Canadian Arctic Archipelago. Although the outflow in the upper layer and the freshwater export respond to short periodic wind events and longer periodic atmospheric circulation patterns, the long-term trend is controlled by the net freshwater supply - the freshwater input minus the ice export. As the ice formation and ice export are expected to diminish in a warmer climate the Canadian Arctic Archipelago, comprising several passages, should gradually carry more of the total Arctic Ocean freshwater outflow. However, the channels in the Canadian Arctic Archipelago discharge into the restricted Baffin, which also receives a part of the Fram Strait freshwater export via the West Greenland Current. In a situation with increased glacial melting and freshwater discharge from Greenland the density of the upper layer in Baffin Bay may decrease considerably. This would reduce the sea level difference between the Arctic Ocean and Baffin Bay and thus weaken the outflow through the Canadian Arctic Archipelago, in extreme cases perhaps even reverse the flow. This would shift the main Arctic Ocean liquid freshwater export from The Canadian Arctic Archipelago to Fram Strait. The zero order dynamics of the exchanges through the Canadian Arctic Archipelago and Baffin Bay are described and the possibility for a weakening of the outflow is examined.

MEMS-based flexible reflective analog modulators (FRAM) for projection displays: a technology review and scale-down study

NASA Astrophysics Data System (ADS)

Picard, Francis; Ilias, Samir; Asselin, Daniel; Boucher, Marc-André; Duchesne, François; Jacob, Michel; Larouche, Carl; Vachon, Carl; Niall, Keith K.; Jerominek, Hubert

2011-02-01

A MEMS based technology for projection display is reviewed. This technology relies on mechanically flexible and reflective microbridges made of aluminum alloy. A linear array of such micromirrors is combined with illumination and Schlieren optics to produce a pixels line. Each microbridge in the array is individually controlled using electrostatic actuation to adjust the pixels intensities. Results of the simulation, fabrication and characterization of these microdevices are presented. Activation voltages below 250 V with response times below 10 μs were obtained for 25 μm × 25 μm micromirrors. With appropriate actuation voltage waveforms, response times of 5 μs and less are achievable. A damage threshold of the mirrors above 8 kW/cm2 has been evaluated. Development of the technology has produced projector engines demonstrating this light modulation principle. The most recent of these engines is DVI compatible and displays VGA video streams at 60 Hz. Recently applications have emerged that impose more stringent requirements on the dimensions of the MEMS array and associated optical system. This triggered a scale down study to evaluate the minimum micromirror size achievable, the impact of this reduced size on the damage threshold and the achievable minimum size of the associated optical system. Preliminary results of this scale down study are reported. FRAM with active surface as small as 5 μm × 5 μm have been investigated. Simulations have shown that such micromirrors could be activated with 107 V to achieve f-number of 1.25. The damage threshold has been estimated for various FRAM sizes. Finally, design of a conceptual miniaturized projector based on 1000×1 array of 5 μm × 5 μm micromirrors is presented. The volume of this projector concept is about 12 cm3.

Fram Strait: Atmospheric Forcing of The Sea Ice Flux

NASA Astrophysics Data System (ADS)

Widell, K.; Østerhus, S.; Gammelsrød, T.

Measuring the magnitude and variability of the ice and freshwater flux through Fram Strait is an important element in understanding climate variability in the Arctic. Since the major part of the ice and freshwater that leaves the Arctic passes through Fram Strait, this passage can be considered a key area for estimating the net ice production in the Arctic Ocean. In 1990, the Norwegian Polar Institute (NPI) started a monitoring program in the strait, most years by means of two moorings with Upward Looking Sonars (ULS) measuring ice draft. From 1995 and on, these moorings were also equipped with Doppler Current Meters (DCM) to measure the ice velocity. These measurements give an opportunity to investigate the different forces affecting ice motion in the strait. Maximum correlation coefficient between atmospheric sea level pressure (from NCEP/NCAR reanalysed data) and southward ice velocity is found when using the cross strait pressure difference along 80N between 10W and 5E (R = 0.72) consider- ing monthly means. Subtracting current velocity at 50 m depth (also measured by the DCM) from ice velocity improves the correlation to R = 0.84. This gives insight in the relative importance of current and wind on the ice motion, and indicates that pressure data can be used to make fairly good estimates of the ice velocity in the strait. In combination with data on ice thickness and ice stream width, this result is used to calculate the ice volume transport. By making assumptions on the parameters in- volved, the time series is extended back to 1948, the start of the pressure record. This time series will be presented and compared to literature, and annual and seasonal vari- ation of the ice flux will be discussed.

Deep ocean ventilation in the Central Fram Strait during the past 35 kyr

NASA Astrophysics Data System (ADS)

Ezat, M.; Rasmussen, T. L.; Skinner, L.; Zamelczyk, K.

2017-12-01

Ocean ventilation in the Arctic Mediterranean via transformation of northward inflowing warm Atlantic surface water into cold deep water affects regional climate, large-scale atmospheric circulation and carbon storage in the deep ocean. Radiocarbon dating of benthic foraminifera has been used to suggest a near-cessation of Arctic Ocean ventilation during the Last Glacial Maximum. During the last deglaciation episodic surges of this Arctic `aged' glacial deep water into the Nordic Seas and the subpolar North Atlantic Ocean may have occurred (Thornalley et al., 2011, 2015; Science). A recent study from the SE Norwegian Sea and the Iceland Basin has revealed large radiocarbon age differences between different benthic foraminiferal species during the last deglaciation (Ezat et al., 2017; Paleoceanography), which arguments for a re-evaluation of previous bottom-water radiocarbon ventilation age reconstructions from the region. Here, we present new species-specific benthic and planktic foraminiferal radiocarbon dates from the central Fram Strait and the SE Norwegian Sea for the past 35 kyr. Several lines of evidence in this new dataset demonstrate that the previously suggested `extreme aging' of >6000 14C years in the Arctic Mediterranean is most likely erroneous. In addition, benthic-planktic age offsets in the deep central Fram Strait display a remarkable decrease from 1300-2300 14C years in late Marine Isotope Stage (MIS) 3 to 0-500 14C year in MIS 2, which correlates with a decrease in benthic d13C and reduction in the benthic-planktic d18O gradient. We are in the process of compiling/screening published ventilation age reconstructions from the Arctic Mediterranean and the subpolar North Atlantic in the light of our new results in order to establish a basin-scale evolution of ocean ventilation since late MIS 3 in this region.

The coccolithophores Emiliania huxleyi and Coccolithus pelagicus: Extant populations from the Norwegian-Iceland Seas and Fram Strait

NASA Astrophysics Data System (ADS)

Dylmer, C. V.; Giraudeau, J.; Hanquiez, V.; Husum, K.

2015-04-01

The distributions of the coccolithophore species Emiliania huxleyi and Coccolithus pelagicus (heterococcolith-bearing phase) in the northern North Atlantic were investigated along two zonal transects crossing Fram Strait and the Norwegian-Iceland Sea, respectively, each conducted during both July 2011 and September-October 2007. Remote-sensing images as well as CTD and ARGO profiles were used to constrain the physico-chemical state of the surface water and surface mixed layer at the time of sampling. Strong seasonal differences in bulk coccolithophore standing stocks characterized the northern and southern transects, where the maximum values of 53×103 cells/l (fall) and 70×103 cells/l (summer), respectively, were essentially explained by E. huxleyi. This pattern confirms previous findings of a summer to fall northwestward shift in peak coccolithophore cell densities within the Nordic Seas. While depicting an overall zonal shift in high cell densities between the summer (Norwegian Sea) and fall (northern Iceland Sea) conditions, the southern transects were additionally characterized by local peak coccolithophore concentrations associated with a geographically and temporally restricted convective process (Lofoten Gyre, summer), as well as an island mass effect (in the vicinity of Jan Mayen Island, fall). Maximum coccolithophore abundances within Fram Strait were found during both seasons close to the western frontal zone (Polar and Arctic Fronts) an area of strong density gradients where physical and chemical properties of the surface mixed layer are prone to enhance phytoplankton biomass and productivity. Here, changes in species dominance from E. huxleyi in summer, to C. pelagicus in fall, were related to the strengthened influence during summer, of surface AW, as well as to high July solar irradiance, within an area usually characterized by C. pelagicus-dominated low density populations.

Isotope concentrations from 24-h urine and 3-h serum samples can be used to measure intestinal magnesium absorption in postmenopausal women

USDA-ARS?s Scientific Manuscript database

Studies suggest a link between magnesium status and osteoporosis. One barrier to more conclusive research on the potential relation is measuring intestinal magnesium absorption (MgA), which requires the use of stable isotopes and a >/= 6-d stool or 3-d urine collection. We evaluated alternative meth...

Characterization of consecutive Streptococcus pyogenes isolates from patients with pharyngitis and bacteriological treatment failure: special reference to prtF1 and sic / drs.

PubMed

Brandt, C M; Allerberger, F; Spellerberg, B; Holland, R; Lütticken, R; Haase, G

2001-02-15

To analyze bacteriological treatment failure in streptococcal pharyngitis, 40 consecutive Streptococcus pyogenes isolates from 18 patients were characterized. For 17 patients, isolates were indistinguishable with respect to emm type, random amplified polymorphic DNA pattern, and presence of prtF1 encoding the fibronectin-binding protein F1. prtF1 was detected only in the 11 isolates (4 patients) with emm12 and in the single isolate with emm6. Further analysis by vir(mga) regulon typing, sequencing of sic encoding the streptococcal inhibitor of complement from 19 isolates with emm1 (9 patients), and sequencing of drs (distantly related sic) from 11 isolates with emm12 revealed distinct sic alleles with insertions and/or deletions in sic that corresponded to differences in restriction patterns of the vir(mga) regulon only for paired isolates of 2 patients. Among isolates with emm12, 2 novel drs alleles were found. Analysis of these data suggests that neither the presence of prtF1 nor the diversification of sic / drs is required for the persistence of S. pyogenes in pharyngitis.

Energetics of alkali and alkaline earth ion-exchanged zeolite A

DOE PAGES

Sun, Hui; Wu, Di; Liu, Kefeng; ...

2016-06-30

Alkali and alkaline earth ion-exchanged zeolite A samples were synthesized in aqueous exchange media. They were thoroughly studied by powder X-ray diffraction (XRD), electron microprobe (EMPA), thermogravimetric analysis and differential scanning calorimetry (TG-DSC), and high temperature oxide melt solution calorimetry. The hydration energetics and enthalpies of formation of these zeolite A materials from constituent oxides were determined. Specifically, the hydration level of zeolite A has a linear dependence on the average ionic potential ( Z/r) of the cation, from 0.894 (Rb-A) to 1.317 per TO 2 (Mg-A). The formation enthalpies from oxides (25 °C) range from –93.71 ± 1.77 (K-A)more » to –48.02 ± 1.85 kJ/mol per TO 2 (Li-A) for hydrated alkali ion-exchanged zeolite A, and from –47.99 ± 1.20 (Ba-A) to –26.41 ± 1.71 kJ/mol per TO 2 (Mg-A) for hydrated alkaline earth ion-exchanged zeolite A. As a result, the formation enthalpy from oxides generally becomes less exothermic as Z/r increases, but a distinct difference in slope is observed between the alkali and the alkaline earth series.« less

Stochastic Simulation Using @ Risk for Dairy Business Investment Decisions

USDA-ARS?s Scientific Manuscript database

A dynamic, stochastic, mechanistic simulation model of a dairy business was developed to evaluate the cost and benefit streams coinciding with technology investments. The model was constructed to embody the biological and economical complexities of a dairy farm system within a partial budgeting fram...

dfnWorks: A discrete fracture network framework for modeling subsurface flow and transport

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hyman, Jeffrey D.; Karra, Satish; Makedonska, Nataliia

DFNWORKS is a parallelized computational suite to generate three-dimensional discrete fracture networks (DFN) and simulate flow and transport. Developed at Los Alamos National Laboratory over the past five years, it has been used to study flow and transport in fractured media at scales ranging from millimeters to kilometers. The networks are created and meshed using DFNGEN, which combines FRAM (the feature rejection algorithm for meshing) methodology to stochastically generate three-dimensional DFNs with the LaGriT meshing toolbox to create a high-quality computational mesh representation. The representation produces a conforming Delaunay triangulation suitable for high performance computing finite volume solvers in anmore » intrinsically parallel fashion. Flow through the network is simulated in dfnFlow, which utilizes the massively parallel subsurface flow and reactive transport finite volume code PFLOTRAN. A Lagrangian approach to simulating transport through the DFN is adopted within DFNTRANS to determine pathlines and solute transport through the DFN. Example applications of this suite in the areas of nuclear waste repository science, hydraulic fracturing and CO 2 sequestration are also included.« less

dfnWorks: A discrete fracture network framework for modeling subsurface flow and transport

DOE PAGES

Hyman, Jeffrey D.; Karra, Satish; Makedonska, Nataliia; ...

2015-11-01

DFNWORKS is a parallelized computational suite to generate three-dimensional discrete fracture networks (DFN) and simulate flow and transport. Developed at Los Alamos National Laboratory over the past five years, it has been used to study flow and transport in fractured media at scales ranging from millimeters to kilometers. The networks are created and meshed using DFNGEN, which combines FRAM (the feature rejection algorithm for meshing) methodology to stochastically generate three-dimensional DFNs with the LaGriT meshing toolbox to create a high-quality computational mesh representation. The representation produces a conforming Delaunay triangulation suitable for high performance computing finite volume solvers in anmore » intrinsically parallel fashion. Flow through the network is simulated in dfnFlow, which utilizes the massively parallel subsurface flow and reactive transport finite volume code PFLOTRAN. A Lagrangian approach to simulating transport through the DFN is adopted within DFNTRANS to determine pathlines and solute transport through the DFN. Example applications of this suite in the areas of nuclear waste repository science, hydraulic fracturing and CO 2 sequestration are also included.« less

Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool.

PubMed

Kallifatidis, Beatrice; Borovička, Jan; Stránská, Jana; Drábek, Jiří; Mills, Deetta K

2014-03-01

The capability of Fluorescent Random Amplified Microsatellites (F-RAMS) to profile hallucinogenic mushrooms to species and sub-species level was assessed. Fifteen samples of Amanita rubescens and 22 samples of other hallucinogenic and non-hallucinogenic mushrooms of the genera Amanita and Psilocybe were profiled using two fluorescently-labeled, 5'degenerate primers, 5'-6FAM-SpC3-DD (CCA)5 and 5'-6FAM-SpC3-DHB (CGA)5, which target different microsatellite repeat regions. Among the two primers, 5'-6FAM-SpC3-DHB (CGA)5 provided more reliable data for identification purposes, by grouping samples of the same species and clustering closely related species together in a dendrogram based on amplicon similarities. A high degree of intra-specific variation between the 15 A. rubescens samples was shown with both primers and the amplicons generated for all A. rubescens samples were organized into three classes of amplicons (discriminant, private, and marker) based on their individualizing potential. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Nondestructive Analysis of MET-5 Paint Can at TA35 Building 2 A-Wing Vault

DOE Office of Scientific and Technical Information (OSTI.GOV)

Desimone, David J.; Vo, Duc Ta

In Building 2 A-wing vault MET-5 has some drums and other packages they wanted NEN-1 help identifying nondestructively. Measurements using a mechanically cooled portable high-purity germanium HPGe Ortec detective were taken of a paint can container labeled DU-2A to determine if any radioactive material was inside. The HPGe detector measures the gamma rays emitted by radioactive material and displays it as a spectrum. The spectrum is used to identify this radioactive material by using appropriate analysis software and identifying the gamma ray peaks. A paint can container, DU-2A, was analyzed with PeakEasy 4.84 and FRAM 5.2. The FRAM report ismore » shown. The enrichment is 0.091% U235 and 99.907% U238. This material is depleted uranium. The measurement was performed in the near field, and to extract a mass a far field measurement will need to be taken.« less

The SEURAT-1 Approach towards Animal Free Human Safety Assessment

EPA Science Inventory

SEURAT-1 is a European public-private research consortium that is working towards animal-free testing of chemical compounds and the highest level of consumer protection. A research strategy was formulated based on the guiding principle to adopt a toxicological mode-of-action fram...

Watershed Central: An Integrated Watershed Assessment and Management Website (Columbus, OH workshop).

EPA Science Inventory

The U.S.EPA’s Office of Water (OW), Office of Research and Development (ORD), and Office of Environmental Information (OEI) have teamed together with other Federal Agencies, state and local agencies, tribal agencies, and non-government organizations, to develop an integrated fram...

Training a Joint and Expeditionary Mindset

DTIC Science & Technology

2006-12-01

associated with the JEM constructs and for using them to create effective computer-mediated training scenarios. The pedagogic model enables development of...ensure the instructional rigor of scenarios and provide a sound basis for determining performance indicators. The pedagogical model enables development...and Subordinate Constructs ........................................................................... 3 Pedagogical Fram ew ork

A conceptual framework that links pollinator foraging behavior to gene flow

USDA-ARS?s Scientific Manuscript database

In insect-pollinated crops such as alfalfa, a better understanding of how pollinator foraging behavior affects gene flow could lead to the development of management strategies to reduce gene flow and facilitate the coexistence of distinct seed-production markets. Here, we introduce a conceptual fram...

The role of summer surface wind anomalies in the summer Arctic sea ice extent in 2010 and 2011

NASA Astrophysics Data System (ADS)

Ogi, M.; Wallace, J. M.

2012-12-01

Masayo Ogi 1 and John M. Wallace 2 masayo.ogi@jamstec.go.jp wallace@atmos.washington.edu 1Japan Agency for Marine-Earth Science and Technology, Yokohama, Japan 2 Department of Atmospheric Sciences, University of Washington, Seattle, Washington The seasonal evolutions of Arctic sea ice extent (SIE) during the summers of 2010 and 2011 are contrasted with that in 2007. The June SIE in 2010 was lower than that in 2007 and was the lowest for that calendar month in the 32-year (1979-2010) record. The September SIE in 2010 would have set a new record low had it not been for the fact that the ice retreated more slowly during the summer months in that year than it did in 2007. Hence from early July onward, the SIE in 2010 remained at levels above those observed in 2007. The SIE minimum in September 2010 proved to be the third lowest on record, eclipsed by values in both 2007 and 2008. In spring and summer of 2011, the Arctic SIE was as low as it was in 2007, but the SIE in September 2011 did not reach record low levels. The SIE minimum in 2011 proved to be the second lowest on record for the period of 1979-2011. Summertime atmospheric conditions play an important role in controlling the variations in Arctic SIE. In a previous study based on statistical analysis of data collected prior to 2007, we showed that anticyclonic summertime circulation anomalies over the Arctic Ocean during the summer months favor low September SIE. We also found that the record-low ice summer year 2007 was characterized by a strong anticyclonic circulation anomaly, accompanied by an Ekman drift of ice out of the marginal seas toward the central Arctic and eventually toward the Fram Strait, as evidenced by the tracks of drifting buoys. Here we assess the extent to which year-to-year differences in summer winds over the Arctic might have contributed to the differing rates of retreat of ice during the summers of 2007, 2010, and 2011. Our results show that the May-June (MJ) pattern in 2010 is characterized by strong anticyclonic wind anomalies over the Arctic Ocean. The corresponding pattern for July-August-September (JAS) is dominated by a cyclonic gyre centered over the Kara Sea. The corresponding patterns for 2007 are weak in MJ and strongly anticyclonic in JAS. The JJA pattern in 2011 is characterized by anticyclonic wind anomalies over the Arctic directed toward the Fram Strait, whereas the September pattern exhibits wind anomalies directed away from the Fram Strait across the central Arctic Ocean toward the Chukchi Sea. The corresponding patterns for 2007 are strongly anticyclonic and directed toward the Fram Strait in both JJA and September. In the absence of the late season push by the winds, the ice did not retreat quite as far in 2011 as it did in 2007. We have shown evidence that low level winds over the Arctic play an important role in mediating the rate of retreat of sea ice during summer. Anomalous anticyclonic flow over the interior of the Arctic directed toward the Fram Strait favors rapid retreat and vice versa. We have argued that the relative rankings of the September SIE for the years 2007, 2010 and 2011 are largely attributable to the differing rates of decrease of SIE during these summers, which are a consequence of year-to-year differences in the seasonal evolution of summertime winds over the Arctic.

Blended near-optimal tools for flexible water resources decision making

NASA Astrophysics Data System (ADS)

Rosenberg, David

2015-04-01

State-of-the-art systems analysis techniques focus on efficiently finding optimal solutions. Yet an optimal solution is optimal only for the static modelled issues and managers often seek near-optimal alternatives that address un-modelled or changing objectives, preferences, limits, uncertainties, and other issues. Early on, Modelling to Generate Alternatives (MGA) formalized near-optimal as performance within a tolerable deviation from the optimal objective function value and identified a few maximally-different alternatives that addressed select un-modelled issues. This paper presents new stratified, Monte Carlo Markov Chain sampling and parallel coordinate plotting tools that generate and communicate the structure and full extent of the near-optimal region to an optimization problem. Plot controls allow users to interactively explore region features of most interest. Controls also streamline the process to elicit un-modelled issues and update the model formulation in response to elicited issues. Use for a single-objective water quality management problem at Echo Reservoir, Utah identifies numerous and flexible practices to reduce the phosphorus load to the reservoir and maintain close-to-optimal performance. Compared to MGA, the new blended tools generate more numerous alternatives faster, more fully show the near-optimal region, help elicit a larger set of un-modelled issues, and offer managers greater flexibility to cope in a changing world.

ToxPlorerTM: A Comprehensive Knowledgebase of Toxicity Pathways Using Ontology-driven Information Extraction

EPA Science Inventory

Realizing the potential of pathway-based toxicity testing requires a fresh look at how we describe phenomena leading to adverse effects in vivo, how we assess them in vitro and how we extrapolate them in silico across chemicals, doses and species. We developed the ToxPlorer™ fram...

Biogeographic patterns of bacterial microdiversity in Arctic deep-sea sediments (HAUSGARTEN, Fram Strait)

PubMed Central

Buttigieg, Pier Luigi; Ramette, Alban

2015-01-01

Marine bacteria colonizing deep-sea sediments beneath the Arctic ocean, a rapidly changing ecosystem, have been shown to exhibit significant biogeographic patterns along transects spanning tens of kilometers and across water depths of several thousand meters (Jacob et al., 2013). Jacob et al. (2013) adopted what has become a classical view of microbial diversity – based on operational taxonomic units clustered at the 97% sequence identity level of the 16S rRNA gene – and observed a very large microbial community replacement at the HAUSGARTEN Long Term Ecological Research station (Eastern Fram Strait). Here, we revisited these data using the oligotyping approach and aimed to obtain new insight into ecological and biogeographic patterns associated with bacterial microdiversity in marine sediments. We also assessed the level of concordance of these insights with previously obtained results. Variation in oligotype dispersal range, relative abundance, co-occurrence, and taxonomic identity were related to environmental parameters such as water depth, biomass, and sedimentary pigment concentration. This study assesses ecological implications of the new microdiversity-based technique using a well-characterized dataset of high relevance for global change biology. PMID:25601856

The role of simulated small-scale ocean variability in inverse computations for ocean acoustic tomography.

PubMed

Dushaw, Brian D; Sagen, Hanne

2017-12-01

Ocean acoustic tomography depends on a suitable reference ocean environment with which to set the basic parameters of the inverse problem. Some inverse problems may require a reference ocean that includes the small-scale variations from internal waves, small mesoscale, or spice. Tomographic inversions that employ data of stable shadow zone arrivals, such as those that have been observed in the North Pacific and Canary Basin, are an example. Estimating temperature from the unique acoustic data that have been obtained in Fram Strait is another example. The addition of small-scale variability to augment a smooth reference ocean is essential to understanding the acoustic forward problem in these cases. Rather than a hindrance, the stochastic influences of the small scale can be exploited to obtain accurate inverse estimates. Inverse solutions are readily obtained, and they give computed arrival patterns that matched the observations. The approach is not ad hoc, but universal, and it has allowed inverse estimates for ocean temperature variations in Fram Strait to be readily computed on several acoustic paths for which tomographic data were obtained.

High biodiversity on a deep-water reef in the eastern Fram Strait.

PubMed

Meyer, Kirstin S; Soltwedel, Thomas; Bergmann, Melanie

2014-01-01

We report on the distribution and abundance of megafauna on a deep-water rocky reef (1796-2373 m) in the Fram Strait, west of Svalbard. Biodiversity and population density are high, with a maximum average of 26.7±0.9 species m(-2) and 418.1±49.6 individuals m(-2) on the east side of the reef summit. These figures contrast with the surrounding abyssal plain fauna, with an average of only 18.1±1.4 species and 29.4±4.3 individuals m(-2) (mean ± standard error). The east side of the reef summit, where the highest richness and density of fauna are found, faces into the predominant bottom current, which likely increases in speed to the summit and serves as a source of particulate food for the numerous suspension feeders present there. We conclude that the observed faunal distribution patterns could be the result of hydrodynamic patterns and food availability above and around the reef. To our knowledge, this study is the first to describe the distribution and diversity of benthic fauna on a rocky reef in deep water.

Results of the Sea Ice Model Intercomparison Project: Evaluation of sea ice rheology schemes for use in climate simulations

NASA Astrophysics Data System (ADS)

Kreyscher, Martin; Harder, Markus; Lemke, Peter; Flato, Gregory M.

2000-05-01

A hierarchy of sea ice rheologies is evaluated on the basis of a comprehensive set of observational data. The investigations are part of the Sea Ice Model Intercomparison Project (SIMIP). Four different sea ice rheology schemes are compared: a viscous-plastic rheology, a cavitating-fluid model, a compressible Newtonian fluid, and a simple free drift approach with velocity correction. The same grid, land boundaries, and forcing fields are applied to all models. As verification data, there are (1) ice thickness data from upward looking sonars (ULS), (2) ice concentration data from the passive microwave radiometers SMMR and SSM/I, (3) daily buoy drift data obtained by the International Arctic Buoy Program (IABP), and (4) satellite-derived ice drift fields based on the 85 GHz channel of SSM/I. All models are optimized individually with respect to mean drift speed and daily drift speed statistics. The impact of ice strength on the ice cover is best revealed by the spatial pattern of ice thickness, ice drift on different timescales, daily drift speed statistics, and the drift velocities in Fram Strait. Overall, the viscous-plastic rheology yields the most realistic simulation. In contrast, the results of the very simple free-drift model with velocity correction clearly show large errors in simulated ice drift as well as in ice thicknesses and ice export through Fram Strait compared to observation. The compressible Newtonian fluid cannot prevent excessive ice thickness buildup in the central Arctic and overestimates the internal forces in Fram Strait. Because of the lack of shear strength, the cavitating-fluid model shows marked differences to the statistics of observed ice drift and the observed spatial pattern of ice thickness. Comparison of required computer resources demonstrates that the additional cost for the viscous-plastic sea ice rheology is minor compared with the atmospheric and oceanic model components in global climate simulations.

Arctic sea ice variability during the last deglaciation: a biomarker approach

NASA Astrophysics Data System (ADS)

Müller, J.; Stein, R. H.

2014-12-01

The last transition from full glacial to current interglacial conditions was accompanied by distinct short-term climate fluctuations caused by changes in the global ocean circulation system. Most palaeoceanographic studies focus on the documentation of the behaviour of the Atlantic Meridional Overturning Circulation (AMOC) during the last deglaciation in response to freshwater forcing events. In this respect, the role of Arctic sea ice remained relatively unconsidered - primarily because of the difficulty of its reconstruction. Here we present new proxy data on late glacial (including the Last Glacial Maximum; LGM) and deglacial sea ice variability in the Arctic Ocean and its main gateway - the Fram Strait - and how these changes in sea ice coverage contributed to AMOC perturbations observed during Heinrich Event 1 and the Younger Dryas. Recurrent short-term advances and retreats of sea ice in Fram Strait, prior and during the LGM, are in line with a variable (or intermittent) North Atlantic heat flow along the eastern corridor of the Nordic Seas. Possibly in direct response to the initial freshwater discharge from melting continental ice-sheets, a permanent sea ice cover established only at about 19 ka BP (i.e. post-LGM) and lasted until 17.6 ka BP, when an abrupt break-up of this thick ice cover and a sudden discharge of huge amounts of sea ice and icebergs through Fram Strait coincided with the weakening of the AMOC during Heinrich Event 1. Similarly, another sea ice maximum at about 12.8 ka BP is associated with the slowdown of the AMOC during the Younger Dryas. The new data sets clearly highlight the important role of Arctic sea ice for the re-organisation of the oceanographic setting in the North Atlantic during the last deglaciation. Further studies and sensitivity experiments to identify crucial driving (and feedback) mechanisms within the High Latitude ice-ocean-atmosphere system will contribute the understanding of rapid climate changes.

Regional and inter-annual variability in Atlantic zooplankton en route to the Arctic Ocean: potential effects of multi-path Atlantic water advection through Fram Strait and the Barents Sea

NASA Astrophysics Data System (ADS)

Kwasniewski, Slawomir; Gluchowska, Marta; Trudnowska, Emilia; Ormanczyk, Mateusz; Walczowski, Waldemar; Beszczynska-Moeller, Agnieszka

2016-04-01

The Arctic is among the regions where the climate change effects on ecosystem will be the most rapid and consequential, with Arctic amplification recognized as an integral part of the process. Great part of the changes are forced by advection of warm waters from the North Atlantic and the expected modifications of Arctic marine ecosystem will be induced not only by changing environmental conditions but also as a result of introducing Atlantic biota. Thus, the knowledge of physical and biological heterogeneity of Atlantic inflow is requisite for understanding the effects of climate change on biological diversity and ecosystem functioning in the Arctic. The complex and variable two-branched structure of the Atlantic Water flow via Fram Strait and the Barents Sea most likely has a strong influence on the ocean biology in these regions, especially in the pelagic realm. Zooplankton are key components of marine ecosystems which form essential links between primary producers and grazer/predator consumers, thus they are important for functioning of the biological carbon pump. Changes in zooplankton distribution and abundance may have cascading effects on ecosystem functioning, with regulatory effects on climate. Based on data collected in summers of 2012-2014, within the scope of the Polish-Norwegian PAVE research project, we investigate zooplankton distribution, abundance and selected structural characteristics of communities, in relation to water mass properties in the Atlantic Water complex flow to the Arctic Ocean. The main questions addressed here are: what are the differences in zooplankton patterns between the Fram Strait and Barents Sea branches, and how does the inter-annual variability of Atlantic Water advection relate to changes in zooplankton? The results of the investigation are precondition for foreseeing changes in the pelagic realm in the Arctic Ocean and are necessary for constructing and tuning plankton components of ecosystem models.

The Very Late Eocene Opening of Fram Strait between the Arctic Ocean and the Nordic Seas: Linkages with the Popigai Impact

NASA Astrophysics Data System (ADS)

Hillaire-Marcel, C.; Poirier, A.

2013-12-01

The transition from the Eocene Arctic Lake to the Arctic Ocean through the opening of Fram Strait, initially dated at ca. 18 Ma based on palynological inferences (Moran et al., 2004, Nature 441, 601-605), has been recently assigned to the very late Eocene (~ 36 Ma) based on Os-isotope stratigraphy and Re-Os isochron ages of sediments from the IODP-ACEX core (Lomonosov Ridge) (Poirier & Hillaire-Marcel, 2011, GRL 38, L14607). Here, we examine the potential linkages of this event with the Popigai meteoritic impact from northeastern Siberia, which has been dated independently at 35.7×0.2 Ma (Bottomley et al. 1997, Nature 338, 365-368). Noteworthy is the fact that in the earliest marine sediments of the ACEX core, the Os-isotope stratigraphy records an isotopic excursion which we tentatively assigned to the chondritic impactor of Popigai. Sr and Pb isotope signatures of detrital sediments (i.e., following the removal of exchangeable fractions) were thus used to further document the sources of terrigenous sediments deposited before, during, and after the transition episode. Above and below the lacustrine/marine boundary, we note relatively constant source provenances (or mixture of sources), implying that relative contributions from regional detrital sedimentary sources, thus relative erosion rates over surrounding continents, did not change much at a Ma-long time scale. On the other hand, a sharp change highlights the lacustrine/marine transition, with an abrupt shift to low values in 87Sr/88Sr, also marked by a (smaller) excursion in all three 204Pb-normalised lead isotopes values (corrected for in-situ decay of U). This isotopic excursion might also be due to the Popigai chondritic Impactor. The impact-related ejection over basaltic target materials would have produced the particulate matter of suitable composition (Wooden et al. 1993, GCA 57, 3677-3704) to account for the isotopic excursion observed. A first order estimate of the Popigai impact yields a value of ~ 10^8 megatons, resulting in a potential seismic event in the Fram Strait area with a magnitude > 10, likely to have resulted in major faulting in the area, thus possibly leading to the opening of the Strait. This very late Eocene opening of Fram Strait, would suggest a potential role of the Arctic basin in the inception of the Atlantic Meridional Overturning Circulation. With this new chronology, the entry of the Earth in the "cold house" system of the Early Oligocene, and the early indices for ice-rafting, might have being nearly synchronous in the Arctic and the circum-Antarctica.

A modelling study of the influence of anomalous wind forcing over the Barents Sea on the Atlantic water flow to the Arctic Ocean in the period 1979-2004

NASA Astrophysics Data System (ADS)

Marciniak, Jakub; Schlichtholz, Pawel; Maslowski, Wieslaw

2016-04-01

Arctic climate system is influenced by oceanic heat transport with the Atlantic water (AW) streaming towards the Arctic Ocean in two branches, through the deep Fram Strait and the shallow Barents Sea. In Fram Strait, the AW submerges below the Polar surface water and then flows cyclonically along the margin of the Arctic Ocean as a subsurface water mass in the Arctic Slope Current. In contrast to the Fram Strait branch, which is the major source of heat for the Arctic Ocean, most of the heat influx to the Barents Sea through the Barents Sea opening (BSO) is passed to the atmosphere. Only cold remnants of AW outflow to the Arctic Ocean through the northeastern gate of the Barents Sea. Some AW entering the Barents Sea recirculates westward, contributing to an outflow from the Barents Sea through the BSO along the shelf slope south of Bear Island, in the Bear Island Slope Current. Even though the two-branched AW flow toward the Arctic Ocean has been known for more than a century, little is known about co-variability of heat fluxes in the two branches, its mechanisms and climatic implications. Recent studies indicate that the Bear Island Slope Current may play a role in this co-variability. Here, co-variability of the flow through the BSO and Fram Strait is investigated using a pan-Arctic coupled ice-ocean hindcast model run for the period 1979-2004 and forced with daily atmospheric data from the ECMWF. Significant wintertime co-variability between the volume transport in the Bear Island and Arctic slope currents and its link to wind forcing over the Barents Sea is confirmed. It is found that the volume transports in these currents are, however, not correlated in the annual mean and that the wintertime co-variability of these currents has no immediate effect on either the net heat flux through the BSO or the net heat flux divergence in the Barents Sea. It is shown that the main climatic effect of wind forcing over the northern Barents Sea shelf is to induce temperature anomalies in the Murman/West Novaya Zemlya current system on the eastern side of the Barents Sea. These anomalies affect sea ice in the eastern Barents Sea 1-3 months later, but are not completely lost on the interactions with the sea ice and local atmosphere. Statistically significant subsurface temperature anomalies driven by anomalous winds over the Barents Sea join, on their exit to the Arctic Ocean through St. Anna Trough, the Arctic Slope Current, in which they persist for several years.

Vascular Endothelial Growth Factor (VEGF) mRNA Isoforms are Altered in Bovine Granulosa Cells (GC) by Circulating Progestin Concentrations (P4) and May Indicate Follicle Status and Oocyte Competence

USDA-ARS?s Scientific Manuscript database

Previously, Melengestrol Acetate (MGA) fed for 14 d (0.5mg/cow/d; < 1 ng/ml P4) resulted in persistent follicles with increased size, decreased number of GC/follicular fluid (FF) volume, and less fertile oocytes. An experiment was conducted to determine effects of circulating P4 on amount of mRNA fo...

CHAMPUS (Civilian Health and Medical Program of the Uniformed Services) Reform: Will It Work? An Analysis of the CHAMPUS Reform Initiative.

DTIC Science & Technology

1987-12-01

eterinarians, optometrists, podiatrists, pharmacists , psycologists, nurse practitioners, rhvsician assistants, and others. In addition, over 146,000 enlisted...and the uti -lization data needed for ood resource C. CONTROL OF HEALTH CARE COSTS 1-,- la c ot- regional fixed-price contracts is set forth by the fram

The Coast Artillery Journal. Volume 72, Number 2, February 1930

DTIC Science & Technology

1930-02-01

hmlisfar further study alang this line, we shall also.co.nfine ourselvesto the "cantact" effect of this agent, as distinguished fram what we may call its...study alang the lines we are nGW cansidering. Mustard is an aily liquid af abo.utthe cam-listencyaf heavy lubricating oil. Dispersed either by artillery

Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus

PubMed Central

2014-01-01

Background Transketolase (TKT) is a key enzyme of the pentose phosphate pathway (PPP), the Calvin cycle and the ribulose monophosphate (RuMP) cycle. Bacillus methanolicus is a facultative RuMP pathway methylotroph. B. methanolicus MGA3 harbors two genes putatively coding for TKTs; one located on the chromosome (tkt C ) and one located on the natural occurring plasmid pBM19 (tkt P ). Results Both enzymes were produced in recombinant Escherichia coli, purified and shown to share similar biochemical parameters in vitro. They were found to be active as homotetramers and require thiamine pyrophosphate for catalytic activity. The inactive apoform of the TKTs, yielded by dialysis against buffer containing 10 mM EDTA, could be reconstituted most efficiently with Mn2+ and Mg2+. Both TKTs were thermo stable at physiological temperature (up to 65°C) with the highest activity at neutral pH. Ni2+, ATP and ADP significantly inhibited activity of both TKTs. Unlike the recently characterized RuMP pathway enzymes fructose 1,6-bisphosphate aldolase (FBA) and fructose 1,6-bisphosphatase/sedoheptulose 1,7-bisphosphatase (FBPase/SBPase) from B. methanolicus MGA3, both TKTs exhibited similar kinetic parameters although they only share 76% identical amino acids. The kinetic parameters were determined for the reaction with the substrates xylulose 5-phosphate (TKTC: kcat/KM: 264 s-1 mM-1; TKTP: kcat/KM: 231 s-1 mM) and ribulose 5-phosphate (TKTC: kcat/KM: 109 s-1 mM; TKTP: kcat/KM: 84 s-1 mM) as well as for the reaction with the substrates glyceraldehyde 3-phosphate (TKTC: kcat/KM: 108 s-1 mM; TKTP: kcat/KM: 71 s-1 mM) and fructose 6-phosphate (TKTC kcat/KM: 115 s-1 mM; TKTP: kcat/KM: 448 s-1 mM). Conclusions Based on the kinetic parameters no major TKT of B. methanolicus could be determined. Increased expression of tkt P , but not of tkt C during growth with methanol [J Bacteriol 188:3063–3072, 2006] argues for TKTP being the major TKT relevant in the RuMP pathway. Neither TKT exhibited activity as dihydroxyacetone synthase, as found in methylotrophic yeast, or as the evolutionary related 1-deoxyxylulose-5-phosphate synthase. The biological significance of the two TKTs for B. methanolicus methylotrophy is discussed. PMID:24405865

Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus.

PubMed

Markert, Benno; Stolzenberger, Jessica; Brautaset, Trygve; Wendisch, Volker F

2014-01-09

Transketolase (TKT) is a key enzyme of the pentose phosphate pathway (PPP), the Calvin cycle and the ribulose monophosphate (RuMP) cycle. Bacillus methanolicus is a facultative RuMP pathway methylotroph. B. methanolicus MGA3 harbors two genes putatively coding for TKTs; one located on the chromosome (tkt(C)) and one located on the natural occurring plasmid pBM19 (tkt(P)). Both enzymes were produced in recombinant Escherichia coli, purified and shown to share similar biochemical parameters in vitro. They were found to be active as homotetramers and require thiamine pyrophosphate for catalytic activity. The inactive apoform of the TKTs, yielded by dialysis against buffer containing 10 mM EDTA, could be reconstituted most efficiently with Mn(2+) and Mg(2+). Both TKTs were thermo stable at physiological temperature (up to 65°C) with the highest activity at neutral pH. Ni(2+), ATP and ADP significantly inhibited activity of both TKTs. Unlike the recently characterized RuMP pathway enzymes fructose 1,6-bisphosphate aldolase (FBA) and fructose 1,6-bisphosphatase/sedoheptulose 1,7-bisphosphatase (FBPase/SBPase) from B. methanolicus MGA3, both TKTs exhibited similar kinetic parameters although they only share 76% identical amino acids. The kinetic parameters were determined for the reaction with the substrates xylulose 5-phosphate (TKT(C): kcat/KM: 264 s(-1) mM(-1); TKT(P): kcat/KM: 231 s(-1) mM) and ribulose 5-phosphate (TKT(C): kcat/KM: 109 s(-1) mM; TKT(P): kcat/KM: 84 s(-1) mM) as well as for the reaction with the substrates glyceraldehyde 3-phosphate (TKT(C): kcat/KM: 108 s(-1) mM; TKT(P): kcat/KM: 71 s(-1) mM) and fructose 6-phosphate (TKT(C) kcat/KM: 115 s(-1) mM; TKT(P): kcat/KM: 448 s(-1) mM). Based on the kinetic parameters no major TKT of B. methanolicus could be determined. Increased expression of tkt(P), but not of tkt(C) during growth with methanol [J Bacteriol 188:3063-3072, 2006] argues for TKT(P) being the major TKT relevant in the RuMP pathway. Neither TKT exhibited activity as dihydroxyacetone synthase, as found in methylotrophic yeast, or as the evolutionary related 1-deoxyxylulose-5-phosphate synthase. The biological significance of the two TKTs for B. methanolicus methylotrophy is discussed.

Near-optimal alternative generation using modified hit-and-run sampling for non-linear, non-convex problems

NASA Astrophysics Data System (ADS)

Rosenberg, D. E.; Alafifi, A.

2016-12-01

Water resources systems analysis often focuses on finding optimal solutions. Yet an optimal solution is optimal only for the modelled issues and managers often seek near-optimal alternatives that address un-modelled objectives, preferences, limits, uncertainties, and other issues. Early on, Modelling to Generate Alternatives (MGA) formalized near-optimal as the region comprising the original problem constraints plus a new constraint that allowed performance within a specified tolerance of the optimal objective function value. MGA identified a few maximally-different alternatives from the near-optimal region. Subsequent work applied Markov Chain Monte Carlo (MCMC) sampling to generate a larger number of alternatives that span the near-optimal region of linear problems or select portions for non-linear problems. We extend the MCMC Hit-And-Run method to generate alternatives that span the full extent of the near-optimal region for non-linear, non-convex problems. First, start at a feasible hit point within the near-optimal region, then run a random distance in a random direction to a new hit point. Next, repeat until generating the desired number of alternatives. The key step at each iterate is to run a random distance along the line in the specified direction to a new hit point. If linear equity constraints exist, we construct an orthogonal basis and use a null space transformation to confine hits and runs to a lower-dimensional space. Linear inequity constraints define the convex bounds on the line that runs through the current hit point in the specified direction. We then use slice sampling to identify a new hit point along the line within bounds defined by the non-linear inequity constraints. This technique is computationally efficient compared to prior near-optimal alternative generation techniques such MGA, MCMC Metropolis-Hastings, evolutionary, or firefly algorithms because search at each iteration is confined to the hit line, the algorithm can move in one step to any point in the near-optimal region, and each iterate generates a new, feasible alternative. We use the method to generate alternatives that span the near-optimal regions of simple and more complicated water management problems and may be preferred to optimal solutions. We also discuss extensions to handle non-linear equity constraints.

Aerial Port Location Study

DTIC Science & Technology

1997-09-01

the Uniform Material Movements and Issue Priority System (UMMIPS) is becoming an increasing difficult and expensive challenge . (3) According to...NEWZEALA 25,742 406 71 CUA CUBI POINT BATAAN PHILIPPIN 23,500 10 72 AJR ARVIDSJAUR SWEDEN 22,900 34 73 KPI KAPIT MALAYSIA 21,155 10 74 MGA MANAGUA...600 3 109 BDS BRINDISI ITALY 485 13 110 KUL KUALA LUMPUR INTL MALAYSIA 391 1 111 YES ISTANBUL TURKEY 352 3 112 DOH DOHA QATAR 208 3 113 ZAG

Highly conductive and transparent thin ZnO films prepared in situ in a low pressure system

NASA Astrophysics Data System (ADS)

Ataev, B. M.; Bagamadova, A. M.; Mamedov, V. V.; Omaev, A. K.; Rabadanov, M. R.

1999-03-01

Sucessful preparation of ZnO : M epitaxial thin films (ETF) in situ doped with donor impurity M=Ga, Sn by chemical vapor despsition in a low-pressure system is reported. Highly conductive (up to 10 -4 Ω cm) and transparent ( T>85%) ZnO : M ETF have been successfully produced on single crystal (1012) sapphire substrates. Electrical properties of the films as well as their excition luminescence were studied.

Functional dissociation between action and perception of object shape in developmental visual object agnosia.

PubMed

Freud, Erez; Ganel, Tzvi; Avidan, Galia; Gilaie-Dotan, Sharon

2016-03-01

According to the two visual systems model, the cortical visual system is segregated into a ventral pathway mediating object recognition, and a dorsal pathway mediating visuomotor control. In the present study we examined whether the visual control of action could develop normally even when visual perceptual abilities are compromised from early childhood onward. Using his fingers, LG, an individual with a rare developmental visual object agnosia, manually estimated (perceptual condition) the width of blocks that varied in width and length (but not in overall size), or simply picked them up across their width (grasping condition). LG's perceptual sensitivity to target width was profoundly impaired in the manual estimation task compared to matched controls. In contrast, the sensitivity to object shape during grasping, as measured by maximum grip aperture (MGA), the time to reach the MGA, the reaction time and the total movement time were all normal in LG. Further analysis, however, revealed that LG's sensitivity to object shape during grasping emerged at a later time stage during the movement compared to controls. Taken together, these results demonstrate a dissociation between action and perception of object shape, and also point to a distinction between different stages of the grasping movement, namely planning versus online control. Moreover, the present study implies that visuomotor abilities can develop normally even when perceptual abilities developed in a profoundly impaired fashion. Copyright © 2016 Elsevier Ltd. All rights reserved.

Blended near-optimal alternative generation, visualization, and interaction for water resources decision making

NASA Astrophysics Data System (ADS)

Rosenberg, David E.

2015-04-01

State-of-the-art systems analysis techniques focus on efficiently finding optimal solutions. Yet an optimal solution is optimal only for the modeled issues and managers often seek near-optimal alternatives that address unmodeled objectives, preferences, limits, uncertainties, and other issues. Early on, Modeling to Generate Alternatives (MGA) formalized near-optimal as performance within a tolerable deviation from the optimal objective function value and identified a few maximally different alternatives that addressed some unmodeled issues. This paper presents new stratified, Monte-Carlo Markov Chain sampling and parallel coordinate plotting tools that generate and communicate the structure and extent of the near-optimal region to an optimization problem. Interactive plot controls allow users to explore region features of most interest. Controls also streamline the process to elicit unmodeled issues and update the model formulation in response to elicited issues. Use for an example, single-objective, linear water quality management problem at Echo Reservoir, Utah, identifies numerous and flexible practices to reduce the phosphorus load to the reservoir and maintain close-to-optimal performance. Flexibility is upheld by further interactive alternative generation, transforming the formulation into a multiobjective problem, and relaxing the tolerance parameter to expand the near-optimal region. Compared to MGA, the new blended tools generate more numerous alternatives faster, more fully show the near-optimal region, and help elicit a larger set of unmodeled issues.

Testing the MOND paradigm of modified dynamics with galaxy-galaxy gravitational lensing.

PubMed

Milgrom, Mordehai

2013-07-26

The MOND paradigm of modified dynamics predicts that the asymptotic gravitational potential of an isolated, bounded (baryonic) mass, M, is ϕ(r)=(MGa0)1/2ln(r). Relativistic MOND theories predict that the lensing effects of M are dictated by ϕ(r) as general-relativity lensing is dictated by the Newtonian potential. Thus MOND predicts that the asymptotic Newtonian potential deduced from galaxy-galaxy gravitational lensing will have (1) a logarithmic r dependence, and (2) a normalization (parametrized standardly as 2σ2) that depends only on M: σ=(MGa0/4)1/4. I compare these predictions with recent results of galaxy-galaxy lensing, and find agreement on all counts. For the “blue”-lenses subsample (“spiral” galaxies) MOND reproduces the observations well with an r′-band M/Lr′∼(1–3)(M/L)⊙, and for “red” lenses (“elliptical” galaxies) with M/Lr′∼(3–6)(M/L)⊙, both consistent with baryons only. In contradistinction, Newtonian analysis requires, typically, M/Lr′∼130(M/L)⊙, bespeaking a mass discrepancy of a factor ∼40. Compared with the staple, rotation-curve tests, MOND is here tested in a wider population of galaxies, through a different phenomenon, using relativistic test objects, and is probed to several-times-lower accelerations–as low as a few percent of a0.

Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.

PubMed

Yue, Han; Liu, Yanqiu; Qu, Huanhuan; Ding, Kan

2017-10-01

Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on. Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated. In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide. It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7. We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone. The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method. Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity. Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical Stockpile Disposal Program. Chemical Weapons Movement History Compilation.

DTIC Science & Technology

1987-06-12

Arsenal, Edgewood Arsenal, and Dugway Proving Ground . (2) The Army has transferred agent fram certain munitions into other containers or munitions...Aberdeen Proving Ground , Maryland (Historical Volume). - ~ - - - - -.. , 27. Sea Dump of 700 Tons of Lewisite and Mustard , NAD, Concord, California, 1958... Proving Ground , Maryland (Historical Volumes). 42. SITREP File, SFTCM II, 1980; Chemical Agent Identification Sets (CAIS) Historical File; Information

Clinical Investigation Program Annual Progress Report.

DTIC Science & Technology

1984-09-30

145 80/201 Camparison of Cardiac Output and Left Ventricular Stroke Work Before and After Standard Anesthesia Induction of Patients Unrergoing...Role of Minimal Surgical Debridement in the Treatment of Vesicant Extravasations. (O)(PR) ....... 159 84/200 Prevention of Macular Injury frm High...fram or Help Prevent Bone Injury .(C)(P)(PR) .................... 171 80/302 Rapid Detection of Bacterial Antigens in Patient Specimens Using

Prediction of exercise-mediated changes in metabolic markers by gene polymorphism.

PubMed

Kahara, Toshio; Takamura, Toshinari; Hayakawa, Tetsuo; Nagai, Yukihiro; Yamaguchi, Hiromi; Katsuki, Tatsuo; Katsuki, Ken-ichi; Katsuki, Michio; Kobayashi, Ken-ichi

2002-08-01

The effects of regular physical exercise on obesity-associated metabolic abnormalities vary for each individual. In this study, we investigated whether genotypes of genes associated with obesity can predict the effects of exercise on changes in metabolic markers in healthy men. Healthy Japanese men (n=106) performed the exercise program at 50% of their maximal heart rate for 20-60 min a day, 2-3 days each week for 3 months. The levels of fasting plasma glucose (FPG) and serum leptin significantly decreased after the exercise program. Polymorphisms of the beta3-adrenergic receptor (beta3AR) and uncoupling protein-1 (UCP-1) genes were analyzed with RFLP methods. In the Trp/Trp genotype of the beta3AR gene, the levels of serum leptin, FPG and fructosamine (FrAm) decreased significantly after the exercise program, but not in the Arg/Arg genotype. In the AG heterozygote and the GG homozygote of the UCP-1 gene, FPG and FrAm levels were significantly reduced, respectively. In conclusion, gene polymorphism of the beta3AR and UCP-1 was found to be associated with the exercise-mediated improvement in glucose tolerance and leptin resistance in healthy Japanese men.

­­­­Submarine Mass Wasting on Hovgaard Ridge, Fram Strait, European Arctic

NASA Astrophysics Data System (ADS)

Forwick, M.; Laberg, J. S.; Husum, K.; Gales, J. A.

2015-12-01

Hovgaard Ridge is an 1800 m high bathymetric high in the Fram Strait, the only deep-water gateway between the Arctic Ocean and the other World's oceans. The slopes of the ridge provide evidence of various types of sediment reworking, including 1) up to 12 km wide single and merged slide scars with maximum ~30 m high headwalls and some secondary escarpments; 2) maximum 3 km wide and 130 m deep slide scars with irregular internal morphology, partly narrowing towards the foot of the slope; 3) up to 130 m deep, 1.5 km wide and maximum 8 km long channels/gullies originating from areas of increasing slope angle at the margins of a plateau on top of the ridge. Most slide scars result presumably from retrogressive failure related to weak layers in contourites or ash. The most likely trigger mechanism is seismicity related to tectonic activity within the nearby mid-ocean fracture zone. Gully/channel formation is suggested to result from cascading water masses and/or from sediment gravity flows originating from failure at the slope break after winnowing on the plateau of the ridge.

Enhancement of Combustion and Flame Stabilization Using Transient Non-Equilibrium Plasma

DTIC Science & Technology

2007-03-31

cathode spot D = diameter of MGA device D= diffusivity of i’h species d = diameter of arc dcoected= diametrically corrected diameter of arc d.rwwd... cathode and anode, stabilization (4) respectively (Fig. 1). A wire was attached to the cathode , which was separated from the outer anode by 2 mm at the...smallest gap (point (1) in Fig. 1). The wire spiraled progressively closer to the cathode , where it was attached at the largest gap between the two

Ferroelectric memory evaluation and development system

NASA Astrophysics Data System (ADS)

Bondurant, David W.

Attention is given to the Ramtron FEDS-1, an IBM PC/AT compatible single-board 16-b microcomputer with 8-kbyte program/data memory implemented with nonvolatile ferroelectric dynamic RAM. This is the first demonstration of a new type of solid state nonvolatile read/write memory, the ferroelectric RAM (FRAM). It is suggested that this memory technology will have a significant impact on avionics system performance and reliability.

Connecting Polar Literature with Contemporary Research

NASA Astrophysics Data System (ADS)

Pfirman, S.; Bell, R.; Fowler, C.

2006-12-01

The public is fascinated with polar research, especially when it is linked with exploration, discovery, adventure, isolation, self-reliance, hardship, and exotic landscapes and biota. A powerful way to engage the public in appreciating current research is to link it with accounts of historical expeditions. We have been doing this at Barnard College, Columbia University, since 2003 through "Exploring the Poles," a First Year Seminar. The class investigates polar exploration at the turn of the last century by reading "Farthest North" by Nansen about his adventure with the Fram, "The Last Place on Earth" by Huntford about Scott and Amundsen's race to the South Pole, and "Endurance" by Lansing about Shackleton's journey. To bring polar conditions home for the students, they are assigned to expedition teams for specific years and the fates of the student "expeditions" are based on the actual environmental conditions as well as their own decisions. For example, in the Arctic, student teams navigate satellite images of coastal ice and then freeze their ships into the sea ice north of Siberia, where they then drift with the ice across the pole, re-creating the transit of the Fram. Because the conditions are based on actual data, natural variability causes different outcomes for different years, creating an element of suspense. Fates of the teams vary tremendously: some teams emerge safely as Nansen did at Fram Strait or reach Elephant Island as happened with Shackleton, while others nearly become hopelessly lost in the Arctic Beaufort Gyre or drift out to sea into the Antarctic Circumpolar Current. Interweaving history, science, and stories of past and present expeditions provides a meaningful connection with the otherwise remote polar regions. Evaluation shows that combining historical texts with current data and simulations is an extremely powerful way of engaging non-scientists in science, and explaining the role of science and the environment in decision- making. Students come to understand issues faced by leaders as they deal with environmental uncertainty, and they become interested in science as they find themselves at the mercy of the elements. The interaction between current research and historical experience as well as the physical and social environments has applications in both formal and informal educational venues.

The Statistical Consulting Center for Astronomy (SCCA)

NASA Technical Reports Server (NTRS)

Akritas, Michael

2001-01-01

The process by which raw astronomical data acquisition is transformed into scientifically meaningful results and interpretation typically involves many statistical steps. Traditional astronomy limits itself to a narrow range of old and familiar statistical methods: means and standard deviations; least-squares methods like chi(sup 2) minimization; and simple nonparametric procedures such as the Kolmogorov-Smirnov tests. These tools are often inadequate for the complex problems and datasets under investigations, and recent years have witnessed an increased usage of maximum-likelihood, survival analysis, multivariate analysis, wavelet and advanced time-series methods. The Statistical Consulting Center for Astronomy (SCCA) assisted astronomers with the use of sophisticated tools, and to match these tools with specific problems. The SCCA operated with two professors of statistics and a professor of astronomy working together. Questions were received by e-mail, and were discussed in detail with the questioner. Summaries of those questions and answers leading to new approaches were posted on the Web (www.state.psu.edu/ mga/SCCA). In addition to serving individual astronomers, the SCCA established a Web site for general use that provides hypertext links to selected on-line public-domain statistical software and services. The StatCodes site (www.astro.psu.edu/statcodes) provides over 200 links in the areas of: Bayesian statistics; censored and truncated data; correlation and regression, density estimation and smoothing, general statistics packages and information; image analysis; interactive Web tools; multivariate analysis; multivariate clustering and classification; nonparametric analysis; software written by astronomers; spatial statistics; statistical distributions; time series analysis; and visualization tools. StatCodes has received a remarkable high and constant hit rate of 250 hits/week (over 10,000/year) since its inception in mid-1997. It is of interest to scientists both within and outside of astronomy. The most popular sections are multivariate techniques, image analysis, and time series analysis. Hundreds of copies of the ASURV, SLOPES and CENS-TAU codes developed by SCCA scientists were also downloaded from the StatCodes site. In addition to formal SCCA duties, SCCA scientists continued a variety of related activities in astrostatistics, including refereeing of statistically oriented papers submitted to the Astrophysical Journal, talks in meetings including Feigelson's talk to science journalists entitled "The reemergence of astrostatistics" at the American Association for the Advancement of Science meeting, and published papers of astrostatistical content.

Implementation of the high-order schemes QUICK and LECUSSO in the COMMIX-1C Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sakai, K.; Sun, J.G.; Sha, W.T.

Multidimensional analysis computer programs based on the finite volume method, such as COMMIX-1C, have been commonly used to simulate thermal-hydraulic phenomena in engineering systems such as nuclear reactors. In COMMIX-1C, the first-order schemes with respect to both space and time are used. In many situations such as flow recirculations and stratifications with steep gradient of velocity and temperature fields, however, high-order difference schemes are necessary for an accurate prediction of the fields. For these reasons, two second-order finite difference numerical schemes, QUICK (Quadratic Upstream Interpolation for Convective Kinematics) and LECUSSO (Local Exact Consistent Upwind Scheme of Second Order), have beenmore » implemented in the COMMIX-1C computer code. The formulations were derived for general three-dimensional flows with nonuniform grid sizes. Numerical oscillation analyses for QUICK and LECUSSO were performed. To damp the unphysical oscillations which occur in calculations with high-order schemes at high mesh Reynolds numbers, a new FRAM (Filtering Remedy and Methodology) scheme was developed and implemented. To be consistent with the high-order schemes, the pressure equation and the boundary conditions for all the conservation equations were also modified to be of second order. The new capabilities in the code are listed. Test calculations were performed to validate the implementation of the high-order schemes. They include the test of the one-dimensional nonlinear Burgers equation, two-dimensional scalar transport in two impinging streams, von Karmann vortex shedding, shear driven cavity flow, Couette flow, and circular pipe flow. The calculated results were compared with available data; the agreement is good.« less

MIZEX. A Program for Mesoscale Air-Ice-Ocean Interaction Experiments in Arctic Marginal Ice Zones. II. A Science Plan for a Summer Marginal Ice Zone Experiment in the Fram Strait/Greenland Sea: 1984.

DTIC Science & Technology

1983-05-01

size and thickness characteris- tics. N’ore complete analysis will require combin- ing ice data with data obtained by the oceano - graphic... sol concentration and microwave brightness tem- perature. A long-range aircraft and a light aircraft Hying from Spitzbergen will study mesoscale

Performance Enhancement Of A Low Cost Multimode Fiber Optic Rotation Sensor

NASA Astrophysics Data System (ADS)

Fredricks, Ronald J.; Johnson, Dean R.

1989-02-01

Several fiber optic Sagnac interferometers employing multimode fiber of both high and ffedimiNrrumbers and simple LED light sources, have been designed and built by the authors over the past two years. New results showing improved performance fran that reported at the August '87 SPIE are given in this paper. The ratios of maximum unambiguous rate signal to random 3a drift signal are now in the range 50-150 a performance enhancement of between 4 and 10. We have found that a step index ring rather than a grajled Index one is necess for good driftperformance and that best results are obtained when all the other ring elements (PZT coary il and I/O slitter are also fabricated fram step index fiber. The 3a drifts in our 200 meter 10 cm diameter breadboards, in particular, are around 1°/sec. Using high V number fiber (100 pm/0.29 NA) no static mode mixers are required to desensitize this relatively short sense coil fram environmental pertubations. With unambiguous maxi rum rates on the order of ±200°/sec using simple detection of the MT fundamental signal the performance of these breadboard systems is now as good or better than many law cost "Coriolis" type rate sensors on the market.

a Modified Genetic Algorithm for Finding Fuzzy Shortest Paths in Uncertain Networks

NASA Astrophysics Data System (ADS)

Heidari, A. A.; Delavar, M. R.

2016-06-01

In realistic network analysis, there are several uncertainties in the measurements and computation of the arcs and vertices. These uncertainties should also be considered in realizing the shortest path problem (SPP) due to the inherent fuzziness in the body of expert's knowledge. In this paper, we investigated the SPP under uncertainty to evaluate our modified genetic strategy. We improved the performance of genetic algorithm (GA) to investigate a class of shortest path problems on networks with vague arc weights. The solutions of the uncertain SPP with considering fuzzy path lengths are examined and compared in detail. As a robust metaheuristic, GA algorithm is modified and evaluated to tackle the fuzzy SPP (FSPP) with uncertain arcs. For this purpose, first, a dynamic operation is implemented to enrich the exploration/exploitation patterns of the conventional procedure and mitigate the premature convergence of GA technique. Then, the modified GA (MGA) strategy is used to resolve the FSPP. The attained results of the proposed strategy are compared to those of GA with regard to the cost, quality of paths and CPU times. Numerical instances are provided to demonstrate the success of the proposed MGA-FSPP strategy in comparison with GA. The simulations affirm that not only the proposed technique can outperform GA, but also the qualities of the paths are effectively improved. The results clarify that the competence of the proposed GA is preferred in view of quality quantities. The results also demonstrate that the proposed method can efficiently be utilized to handle FSPP in uncertain networks.

Limonene inhibits streptococcal biofilm formation by targeting surface-associated virulence factors.

PubMed

Subramenium, Ganapathy Ashwinkumar; Vijayakumar, Karuppiah; Pandian, Shunmugiah Karutha

2015-08-01

The present study explores the efficacy of limonene, a cyclic terpene found in the rind of citrus fruits, for antibiofilm potential against species of the genus Streptococcus, which have been deeply studied worldwide owing to their multiple pathogenic efficacy. Limonene showed a concentration-dependent reduction in the biofilm formation of Streptococcus pyogenes (SF370), with minimal biofilm inhibitory concentration (MBIC) of 400 μg ml - 1. Limonene was found to possess about 75-95 % antibiofilm activity against all the pathogens tested, viz. Streptococcus pyogenes (SF370 and 5 clinical isolates), Streptococcus mutans (UA159) and Streptococcus mitis (ATCC 6249) at 400 μg ml - 1 concentration. Microscopic analysis of biofilm architecture revealed a quantitative breach in biofilm formation. Results of a surface-coating assay suggested that the possible mode of action of limonene could be by inhibiting bacterial adhesion to surfaces, thereby preventing the biofilm formation cascade. Susceptibility of limonene-treated Streptococcus pyogenes to healthy human blood goes in unison with gene expression studies in which the mga gene was found to be downregulated. Anti-cariogenic efficacy of limonene against Streptococcus mutans was confirmed, with inhibition of acid production and downregulation of the vicR gene. Downregulation of the covR, mga and vicR genes, which play a critical role in regulating surface-associated proteins in Streptococcus pyogenes and Streptococcus mutans, respectively, is yet further evidence to show that limonene targets surface-associated proteins. The results of physiological assays and gene expression studies clearly show that the surface-associated antagonistic mechanism of limonene also reduces surface-mediated virulence factors.

Rare-earth transition-metal gallium chalcogenides RE3MGaCh7 (M=Fe, Co, Ni; Ch=S, Se)

NASA Astrophysics Data System (ADS)

Rudyk, Brent W.; Stoyko, Stanislav S.; Oliynyk, Anton O.; Mar, Arthur

2014-02-01

Six series of quaternary rare-earth transition-metal chalcogenides RE3MGaCh7 (M=Fe, Co, Ni; Ch=S, Se), comprising 33 compounds in total, have been prepared by reactions of the elements at 1050 °C (for the sulphides) or 900 °C (for the selenides). They adopt noncentrosymmetric hexagonal structures (ordered Ce3Al1.67S7-type, space group P63, Z=2) with cell parameters in the ranges of a=9.5-10.2 Å and c=6.0-6.1 Å for the sulphides and a=10.0-10.5 Å and c=6.3-6.4 Å for the selenides as refined from powder X-ray diffraction data. Single-crystal structures were determined for five members of the sulphide series RE3FeGaS7 (RE=La, Pr, Tb) and RE3CoGaS7 (RE=La, Tb). The highly anisotropic crystal structures consist of one-dimensional chains of M-centred face-sharing octahedra and stacks of Ga-centred tetrahedra all pointing in the same direction. Magnetic measurements on the sulphides reveal paramagnetic behaviour in some cases and long-range antiferromagnetic behaviour with low Néel temperatures (15 K or lower) in others. Ga L-edge XANES spectra support the presence of highly cationic Ga tetrahedral centres with a tendency towards more covalent Ga-Ch character on proceeding from the sulphides to the selenides. Band structure calculations on La3FeGaS7 indicate that the electronic structure is dominated by Fe 3d-based states near the Fermi level.

Magnetization and anisotropy of cobalt ferrite thin films

NASA Astrophysics Data System (ADS)

Eskandari, F.; Porter, S. B.; Venkatesan, M.; Kameli, P.; Rode, K.; Coey, J. M. D.

2017-12-01

The magnetization of thin films of cobalt ferrite frequently falls far below the bulk value of 455 kA m-1 , which corresponds to an inverse cation distribution in the spinel structure with a significant orbital moment of about 0.6 μB that is associated with the octahedrally coordinated Co2+ ions. The orbital moment is responsible for the magnetostriction and magnetocrystalline anisotropy and its sensitivity to imposed strain. We have systematically investigated the structure and magnetism of films produced by pulsed-laser deposition on different substrates (Ti O2 , MgO, MgA l2O4 , SrTi O3 , LSAT, LaAl O3 ) and as a function of temperature (500 -700 °C) and oxygen pressure (10-4-10 Pa ) . Magnetization at room-temperature ranges from 60 to 440 kA m-1 , and uniaxial substrate-induced anisotropy ranges from +220 kJ m-3 for films on deposited on MgO (100) to -2100 kJ m-3 for films deposited on MgA l2O4 (100), where the room-temperature anisotropy field reaches 14 T. No rearrangement of high-spin Fe3+ and Co2+ cations on tetrahedral and octahedral sites can reduce the magnetization below the bulk value, but a switch from Fe3+ and Co2+ to Fe2+ and low-spin Co3+ on octahedral sites will reduce the low-temperature magnetization to 120 kA m-1 , and a consequent reduction of Curie temperature can bring the room-temperature value to near zero. Possible reasons for the appearance of low-spin cobalt in the thin films are discussed.

l-lysine production by Bacillus methanolicus: Genome-based mutational analysis and l-lysine secretion engineering.

PubMed

Nærdal, Ingemar; Netzer, Roman; Irla, Marta; Krog, Anne; Heggeset, Tonje Marita Bjerkan; Wendisch, Volker F; Brautaset, Trygve

2017-02-20

Bacillus methanolicus is a methylotrophic bacterium with an increasing interest in academic research and for biotechnological applications. This bacterium was previously applied for methanol-based production of l-glutamate, l-lysine and the five-carbon diamine cadaverine by wild type, classical mutant and recombinant strains. The genomes of two different l-lysine secreting B. methanolicus classical mutant strains, NOA2#13A52-8A66 and M168-20, were sequenced. We focused on mutational mapping in genes present in l-lysine and other relevant amino acid biosynthetic pathways, as well as in the primary cell metabolism important for precursor supply. In addition to mutations in the aspartate pathway genes dapG, lysA and hom-1, new mutational target genes like alr, proA, proB1, leuC, odhA and pdhD were identified. Surprisingly, no mutations were found in the putative l-lysine transporter gene lysE MGA3 . Inspection of the wild type B. methanolicus strain PB1 genome sequence identified two homologous putative l-lysine transporter genes, lysE PB1 and lysE2 PB1 . The biological role of these putative l-lysine transporter genes, together with the heterologous l-lysine exporter gene lysE Cg from Corynebacterium glutamicum, were therefore investigated. Our results demonstrated that the titer of secreted l-lysine in B. methanolicus was significantly increased by overexpression of lysE Cg while overexpression of lysE MGA3 , lysE PB1 and lysE2 PB1 had no measurable effect. Copyright © 2017 Elsevier B.V. All rights reserved.

Issues of Commonality. Volume II. Issue Analysis.

DTIC Science & Technology

1980-12-01

lubricating oil, and residual oil. 68 ° B Diesel fuel and kerosene make up what are known as the middle distillates. Jet fuels are blends from this...group of distillates which include some gasoline and some heavier oils. The range of hydrocarbons selected for this blending determines the "cut" of...the fuel. Wide-cut fuels are blended fram a wide range of hydrocarbons; narrow-cut fuels include only a small range of hydrocarbons from the

Radiation-Induced Gastrointestinal Death in the Monkey

DTIC Science & Technology

1959-06-01

muscularis mucosa FIGURE 9 could be identified in the histologic prep- One of the more severe cases of atrophic gastritis . arations. The bodies of the glands...sources into position and then was lowered rapidly The histologic preparations examined in this into the center of the field. The dose rate was 803 r...tributed over a spherical wire fram%. 𔄀 inches and one from the rectum. All histologic inter- in diameter. The dose rate at the center of pretations were

Toward Quantifying the Impact of Atmospheric Forcing on Arctic Sea Ice Variability Using the NPS 1/12 Degree Pan-Arctic Coupled Ice-Ocean Model

DTIC Science & Technology

2010-03-01

strong while the temperatures over Scandinavia and Europe (eastern Arctic) are warmer and winds are weaker than average (Serreze and Barry 2005...than the Fram Strait branch than previously thought. This could facilitate an increase in the frequency of storms reaching higher latitudes...REFERENCES Ackerman, J. T., 2008: Climate Change, National Security, and the Quadrennial Defense Review: Avoiding the Perfect Storm . Strategic Studies

apGA: An adaptive parallel genetic algorithm

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liepins, G.E.; Baluja, S.

1991-01-01

We develop apGA, a parallel variant of the standard generational GA, that combines aggressive search with perpetual novelty, yet is able to preserve enough genetic structure to optimally solve variably scaled, non-uniform block deceptive and hierarchical deceptive problems. apGA combines elitism, adaptive mutation, adaptive exponential scaling, and temporal memory. We present empirical results for six classes of problems, including the DeJong test suite. Although we have not investigated hybrids, we note that apGA could be incorporated into other recent GA variants such as GENITOR, CHC, and the recombination stage of mGA. 12 refs., 2 figs., 2 tabs.

Image Understanding Research and Its Application to Cartography and Computer-Based Analysis of Aerial Imagery

DTIC Science & Technology

1983-05-01

Parallel Computation that Assign Canonical Object-Based Frames of Refer- ence," Proc. 7th it. .nt. Onf. on Artifcial Intellig nce (IJCAI-81), Vol. 2...Perception of Linear Struc- ture in Imaged Data ." TN 276, Artiflci!.a Intelligence Center, SRI International, Feb. 1983. [Fram75] J.P. Frain and E.S...1983 May 1983 D C By: Martin A. Fischler, Program Director S ELECTE Principal Investigator, (415)859-5106 MAY 2 21990 Artificial Intelligence Center

Arctic Physical Oceanography.

DTIC Science & Technology

1984-07-01

series of fluActuations. a 1 ate velocity was recorded -’ith bot "h fixed and rofig current meter systems . Current v eters eaere rigidly attached to 750-r...with the U.S. Navy Transit satellite navigation system two per hour. II _20. 00 " 00 Ice slotion - 5i 850 FRAM-I drill frock 0F 1500 Monned floe o...kilometers of such survey line was flown. system . In addition 200 km of continuous aeromagnetic lines Both sea ice and water were sampled for later were

DFT investigations of hydrogen storage materials

NASA Astrophysics Data System (ADS)

Wang, Gang

Hydrogen serves as a promising new energy source having no pollution and abundant on earth. However the most difficult problem of applying hydrogen is to store it effectively and safely, which is smartly resolved by attempting to keep hydrogen in some metal hydrides to reach a high hydrogen density in a safe way. There are several promising metal hydrides, the thermodynamic and chemical properties of which are to be investigated in this dissertation. Sodium alanate (NaAlH4) is one of the promising metal hydrides with high hydrogen storage capacity around 7.4 wt. % and relatively low decomposition temperature of around 100 °C with proper catalyst. Sodium hydride is a product of the decomposition of NaAlH4 that may affect the dynamics of NaAlH4. The two materials with oxygen contamination such as OH- may influence the kinetics of the dehydriding/rehydriding processes. Thus the solid solubility of OH - groups (NaOH) in NaAlH4 and NaH is studied theoretically by DFT calculations. Magnesium boride [Mg(BH4)2] is has higher hydrogen capacity about 14.9 wt. % and the decomposition temparture of around 250 °C. However one flaw restraining its application is that some polyboron compounds like MgB12H12 preventing from further release of hydrogen. Adding some transition metals that form magnesium transition metal ternary borohydride [MgaTMb(BH4)c] may simply the decomposition process to release hydrogen with ternary borides (MgaTMbBc). The search for the probable ternary borides and the corresponding pseudo phase diagrams as well as the decomposition thermodynamics are performed using DFT calculations and GCLP method to present some possible candidates.

On the evolution of handedness: evidence for feeding biases.

PubMed

Flindall, Jason W; Gonzalez, Claudia L R

2013-01-01

Many theories have been put forward to explain the origins of right-handedness in humans. Here we present evidence that this preference may stem in part from a right hand advantage in grasping for feeding. Thirteen participants were asked to reach-to-grasp food items of 3 different sizes: SMALL (Cheerios®), MEDIUM (Froot Loops®), and LARGE (Oatmeal Squares®). Participants used both their right- and left-hands in separate blocks (50 trials each, starting order counterbalanced) to grasp the items. After each grasp, participants either a) ate the food item, or b) placed it inside a bib worn beneath his/her chin (25 trials each, blocked design, counterbalanced). The conditions were designed such that the outward and inward movement trajectories were similar, differing only in the final step of placing it in the mouth or bib. Participants wore Plato liquid crystal goggles that blocked vision between trials. All trials were conducted in closed-loop with 5000 ms of vision. Hand kinematics were recorded by an Optotrak Certus, which tracked the position of three infrared diodes attached separately to the index finger, thumb, and wrist. We found a task (EAT/PLACE) by hand (LEFT/RIGHT) interaction on maximum grip aperture (MGA; the maximum distance between the index finger and thumb achieved during grasp pre-shaping). MGAs were smaller during right-handed movements, but only when grasping with intent to eat. Follow-up tests show that the RIGHT-HAND/EAT MGA was significantly smaller than all other hand/task conditions. Because smaller grip apertures are typically associated with greater precision, our results demonstrate a right-hand advantage for the grasp-to-eat movement. From an evolutionary perspective, early humans may have preferred the hand that could grasp food with more precision, thereby maximizing the likelihood of retrieval, consumption, and consequently, survival.

Dissolved Organic Matter Assisted Transport of Hormones Through An Agricultural Soil

NASA Astrophysics Data System (ADS)

Jann, S.; Totsche, K. U.; Koegel-Knabner, I.; Schiffer, B.; Meyer, H. H. D.

In the last years the disrupting activity of steroidal sex hormones like estrogens has been discussed for various ecosystems and even for human fertility. Once released into the environment, steroids pose a severe risk to fauna and man. After excretion of the relevant compounds or their metabolites by the target animals, the transition of biologically active substances via dung or manure onto soils and into the groundwa- ter cannot be excluded. Yet there is only little knowledge on the stability, degradation and transport pathways of steroids in soils. Just as little is known about the fate of anabolic steroids which are licensed as growth promotants for farm animals in many meat-exporting countries outside the EU (e.g. USA, Australia). We therefore studied the transport of Trenbolone-17 and Melengestrolacetate (MGA) with col- umn experiments employing aggregated agricultural field soil materials (Luvisol E and Bt horizons). The columns (14.6 cm in height, 4.7 cm in diameter) were perco- lated from bottom to top using a peristaltic pump. The mean volumetric flow rate was kept constant throughout the experiments at 20 ml h-1. Chloride was used as nonreac- tive tracer. The flow regime is controlled by two flow regions reflecting the dual mode pore size distribution of the aggregated soil material. Our results show that although the very high KOC values U Trenbolone: 24311 within the E-horizon; 21622 within the Bt-horizon and MGA: 16708 within the E-horizon; 59459 within the Bt horizon - we observe a quick breakthrough of low concentrations of the hormones simultaneous with the non-reactive tracer chloride. This points to the fact that within aggregated field soil, the risk for deep seepage of low concentrations of hormones is high.

Genome sequence of thermotolerant Bacillus methanolicus: features and regulation related to methylotrophy and production of L-lysine and L-glutamate from methanol.

PubMed

Heggeset, Tonje M B; Krog, Anne; Balzer, Simone; Wentzel, Alexander; Ellingsen, Trond E; Brautaset, Trygve

2012-08-01

Bacillus methanolicus can utilize methanol as its sole carbon and energy source, and the scientific interest in this thermotolerant bacterium has focused largely on exploring its potential as a biocatalyst for the conversion of methanol into L-lysine and L-glutamate. We present here the genome sequences of the important B. methanolicus model strain MGA3 (ATCC 53907) and the alternative wild-type strain PB1 (NCIMB13113). The physiological diversity of these two strains was demonstrated by a comparative fed-batch methanol cultivation displaying highly different methanol consumption and respiration profiles, as well as major differences in their L-glutamate production levels (406 mmol liter(-1) and 11 mmol liter(-1), respectively). Both genomes are small (ca 3.4 Mbp) compared to those of other related bacilli, and MGA3 has two plasmids (pBM19 and pBM69), while PB1 has only one (pBM20). In particular, we focus here on genes representing biochemical pathways for methanol oxidation and concomitant formaldehyde assimilation and dissimilation, the important phosphoenol pyruvate/pyruvate anaplerotic node, the tricarboxylic acid cycle including the glyoxylate pathway, and the biosynthetic pathways for L-lysine and L-glutamate. Several unique findings were made, including the discovery of three different methanol dehydrogenase genes in each of the two B. methanolicus strains, and the genomic analyses were accompanied by gene expression studies. Our results provide new insight into a number of peculiar physiological and metabolic traits of B. methanolicus and open up possibilities for system-level metabolic engineering of this bacterium for the production of amino acids and other useful compounds from methanol.

Functional Characterization of Key Enzymes involved in l-Glutamate Synthesis and Degradation in the Thermotolerant and Methylotrophic Bacterium Bacillus methanolicus

PubMed Central

Krog, Anne; Heggeset, Tonje Marita Bjerkan; Ellingsen, Trond Erling

2013-01-01

Bacillus methanolicus wild-type strain MGA3 secretes 59 g/liter−1 of l-glutamate in fed-batch methanol cultivations at 50°C. We recently sequenced the MGA3 genome, and we here characterize key enzymes involved in l-glutamate synthesis and degradation. One glutamate dehydrogenase (GDH) that is encoded by yweB and two glutamate synthases (GOGATs) that are encoded by the gltAB operon and by gltA2 were found, in contrast to Bacillus subtilis, which has two different GDHs and only one GOGAT. B. methanolicus has a glutamine synthetase (GS) that is encoded by glnA and a 2-oxoglutarate dehydrogenase (OGDH) that is encoded by the odhAB operon. The yweB, gltA, gltB, and gltA2 gene products were purified and characterized biochemically in vitro. YweB has a low Km value for ammonium (10 mM) and a high Km value for l-glutamate (250 mM), and the Vmax value is 7-fold higher for l-glutamate synthesis than for the degradation reaction. GltA and GltA2 displayed similar Km values (1 to 1.4 mM) and Vmax values (4 U/mg) for both l-glutamate and 2-oxoglutarate as the substrates, and GltB had no effect on the catalytic activities of these enzymes in vitro. Complementation assays indicated that GltA and not GltA2 is dependent on GltB for GOGAT activity in vivo. To our knowledge, this is the first report describing the presence of two active GOGATs in a bacterium. In vivo experiments indicated that OGDH activity and, to some degree, GOGAT activity play important roles in regulating l-glutamate production in this organism. PMID:23811508

Functional characterization of key enzymes involved in L-glutamate synthesis and degradation in the thermotolerant and methylotrophic bacterium Bacillus methanolicus.

PubMed

Krog, Anne; Heggeset, Tonje Marita Bjerkan; Ellingsen, Trond Erling; Brautaset, Trygve

2013-09-01

Bacillus methanolicus wild-type strain MGA3 secretes 59 g/liter(-1) of l-glutamate in fed-batch methanol cultivations at 50°C. We recently sequenced the MGA3 genome, and we here characterize key enzymes involved in l-glutamate synthesis and degradation. One glutamate dehydrogenase (GDH) that is encoded by yweB and two glutamate synthases (GOGATs) that are encoded by the gltAB operon and by gltA2 were found, in contrast to Bacillus subtilis, which has two different GDHs and only one GOGAT. B. methanolicus has a glutamine synthetase (GS) that is encoded by glnA and a 2-oxoglutarate dehydrogenase (OGDH) that is encoded by the odhAB operon. The yweB, gltA, gltB, and gltA2 gene products were purified and characterized biochemically in vitro. YweB has a low Km value for ammonium (10 mM) and a high Km value for l-glutamate (250 mM), and the Vmax value is 7-fold higher for l-glutamate synthesis than for the degradation reaction. GltA and GltA2 displayed similar Km values (1 to 1.4 mM) and Vmax values (4 U/mg) for both l-glutamate and 2-oxoglutarate as the substrates, and GltB had no effect on the catalytic activities of these enzymes in vitro. Complementation assays indicated that GltA and not GltA2 is dependent on GltB for GOGAT activity in vivo. To our knowledge, this is the first report describing the presence of two active GOGATs in a bacterium. In vivo experiments indicated that OGDH activity and, to some degree, GOGAT activity play important roles in regulating l-glutamate production in this organism.

Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for L-lysine production from methanol at 50 degrees C.

PubMed

Brautaset, Trygve; Jakobsen, Øyvind M; Degnes, Kristin F; Netzer, Roman; Naerdal, Ingemar; Krog, Anne; Dillingham, Rick; Flickinger, Michael C; Ellingsen, Trond E

2010-07-01

We here present the pyc gene encoding pyruvate carboxylase (PC), and the hom-1 and hom-2 genes encoding two active homoserine dehydrogenase (HD) proteins, in methylotrophic Bacillus methanolicus MGA3. In general, both PC and HD are regarded as key targets for improving bacterial L-lysine production; PC plays a role in precursor oxaloacetate (OAA) supply while HD controls an important branch point in the L-lysine biosynthetic pathway. The hom-1 and hom-2 genes were strongly repressed by L-threonine and L-methionine, respectively. Wild-type MGA3 cells secreted 0.4 g/l L-lysine and 59 g/l L-glutamate under optimised fed batch methanol fermentation. The hom-1 mutant M168-20 constructed herein secreted 11 g/l L-lysine and 69 g/l of L-glutamate, while a sixfold higher L-lysine overproduction (65 g/l) of the previously constructed classical B. methanolicus mutant NOA2#13A52-8A66 was accompanied with reduced L-glutamate production (28 g/l) and threefold elevated pyc transcription level. Overproduction of PC and its mutant enzyme P455S in M168-20 had no positive effect on the volumetric L-lysine yield and the L-lysine yield on methanol, and caused significantly reduced volumetric L-glutamate yield and L: -glutamate yield on methanol. Our results demonstrated that hom-1 represents one key target for achieving L-lysine overproduction, PC activity plays an important role in controlling L-glutamate production from methanol, and that OAA precursor supply is not a major bottleneck for L-lysine overproduction by B. methanolicus.

Plasmid-dependent methylotrophy in thermotolerant Bacillus methanolicus.

PubMed

Brautaset, Trygve; Jakobsen M, Øyvind M; Flickinger, Michael C; Valla, Svein; Ellingsen, Trond E

2004-03-01

Bacillus methanolicus can efficiently utilize methanol as a sole carbon source and has an optimum growth temperature of 50 degrees C. With the exception of mannitol, no sugars have been reported to support rapid growth of this organism, which is classified as a restrictive methylotroph. Here we describe the DNA sequence and characterization of a 19,167-bp circular plasmid, designated pBM19, isolated from B. methanolicus MGA3. Sequence analysis of pBM19 demonstrated the presence of the methanol dehydrogenase gene, mdh, which is crucial for methanol consumption in this bacterium. In addition, five genes (pfk, encoding phosphofructokinase; rpe, encoding ribulose-5-phosphate 3-epimerase; tkt, encoding transketolase; glpX, encoding fructose-1,6-bisphosphatase; and fba, encoding fructose-1,6-bisphosphate aldolase) with deduced roles in methanol assimilation via the ribulose monophosphate pathway are encoded by pBM19. A shuttle vector, pTB1.9, harboring the pBM19 minimal replicon (repB and ori) was constructed and used to transform MGA3. Analysis of the resulting recombinant strain demonstrated that it was cured of pBM19 and was not able to grow on methanol. A pTB1.9 derivative harboring the complete mdh gene could not restore growth on methanol when it was introduced into the pBM19-cured strain, suggesting that additional pBM19 genes are required for consumption of this carbon source. Screening of 13 thermotolerant B. methanolicus wild-type strains showed that they all harbor plasmids similar to pBM19, and this is the first report describing plasmid-linked methylotrophy in any microorganism. Our findings should have an effect on future genetic manipulations of this organism, and they contribute to a new understanding of the biology of methylotrophs.

Genome Sequence of Thermotolerant Bacillus methanolicus: Features and Regulation Related to Methylotrophy and Production of l-Lysine and l-Glutamate from Methanol

PubMed Central

Heggeset, Tonje M. B.; Krog, Anne; Balzer, Simone; Wentzel, Alexander; Ellingsen, Trond E.

2012-01-01

Bacillus methanolicus can utilize methanol as its sole carbon and energy source, and the scientific interest in this thermotolerant bacterium has focused largely on exploring its potential as a biocatalyst for the conversion of methanol into l-lysine and l-glutamate. We present here the genome sequences of the important B. methanolicus model strain MGA3 (ATCC 53907) and the alternative wild-type strain PB1 (NCIMB13113). The physiological diversity of these two strains was demonstrated by a comparative fed-batch methanol cultivation displaying highly different methanol consumption and respiration profiles, as well as major differences in their l-glutamate production levels (406 mmol liter−1 and 11 mmol liter−1, respectively). Both genomes are small (ca 3.4 Mbp) compared to those of other related bacilli, and MGA3 has two plasmids (pBM19 and pBM69), while PB1 has only one (pBM20). In particular, we focus here on genes representing biochemical pathways for methanol oxidation and concomitant formaldehyde assimilation and dissimilation, the important phosphoenol pyruvate/pyruvate anaplerotic node, the tricarboxylic acid cycle including the glyoxylate pathway, and the biosynthetic pathways for l-lysine and l-glutamate. Several unique findings were made, including the discovery of three different methanol dehydrogenase genes in each of the two B. methanolicus strains, and the genomic analyses were accompanied by gene expression studies. Our results provide new insight into a number of peculiar physiological and metabolic traits of B. methanolicus and open up possibilities for system-level metabolic engineering of this bacterium for the production of amino acids and other useful compounds from methanol. PMID:22610424

Plasmid-Dependent Methylotrophy in Thermotolerant Bacillus methanolicus

PubMed Central

Brautaset, Trygve; Jakobsen, Øyvind M.; Flickinger, Michael C.; Valla, Svein; Ellingsen, Trond E.

2004-01-01

Bacillus methanolicus can efficiently utilize methanol as a sole carbon source and has an optimum growth temperature of 50°C. With the exception of mannitol, no sugars have been reported to support rapid growth of this organism, which is classified as a restrictive methylotroph. Here we describe the DNA sequence and characterization of a 19,167-bp circular plasmid, designated pBM19, isolated from B. methanolicus MGA3. Sequence analysis of pBM19 demonstrated the presence of the methanol dehydrogenase gene, mdh, which is crucial for methanol consumption in this bacterium. In addition, five genes (pfk, encoding phosphofructokinase; rpe, encoding ribulose-5-phosphate 3-epimerase; tkt, encoding transketolase; glpX, encoding fructose-1,6-bisphosphatase; and fba, encoding fructose-1,6-bisphosphate aldolase) with deduced roles in methanol assimilation via the ribulose monophosphate pathway are encoded by pBM19. A shuttle vector, pTB1.9, harboring the pBM19 minimal replicon (repB and ori) was constructed and used to transform MGA3. Analysis of the resulting recombinant strain demonstrated that it was cured of pBM19 and was not able to grow on methanol. A pTB1.9 derivative harboring the complete mdh gene could not restore growth on methanol when it was introduced into the pBM19-cured strain, suggesting that additional pBM19 genes are required for consumption of this carbon source. Screening of 13 thermotolerant B. methanolicus wild-type strains showed that they all harbor plasmids similar to pBM19, and this is the first report describing plasmid-linked methylotrophy in any microorganism. Our findings should have an effect on future genetic manipulations of this organism, and they contribute to a new understanding of the biology of methylotrophs. PMID:14973041

Branching of Atlantic Water within the Greenland-Spitsbergen Passage: An estimate of recirculation

NASA Astrophysics Data System (ADS)

Manley, T. O.

1995-10-01

Atlantic Water (AW) supplies the largest source of heat, mass, and salt to the Arctic Ocean via Fram Strait (Greenland-Spitsbergen Passage), yet it represents only a fraction of the Atlantic Water that resides in the Greenland, Iceland, Norwegian, and Barents Seas. This is a result of both the branching of the central core of AW along its northward flow and the modification of its T-S signature through air-sea-ice interactions and internal mixing. This paper addresses the quantitative analysis of the three dominant Atlantic Water cores within Fram Strait and north of 76°N using an 11-year (1977 to 1987) hydrographic database. Spatial variations of heat, volume, and salt along its flow path of some 600 km showed that the major core of Atlantic Water that directly enters the Arctic Ocean (Svalbard branch) did not extend past 20°E. Of the 9719 km3 of Atlantic Water existing within the region, one third resided within the Svalbard branch; the remainder, 22% and 45%, were held within the Return Atlantic Current and the Yermak branches, respectively. Restricting the analysis to a southern limit of 79°N effectively removed the Return Atlantic Current and showed a nearly equal split between the two remaining branches. Work completed by Bourke et al. (1988) indicated that the Yermak branch is largely recirculated to the south; if true, this analysis supports Rudels' (1987) model estimate of a 50% recirculation of AW within this region.

Arctic Ocean Freshwater: How Robust are Model Simulations

NASA Technical Reports Server (NTRS)

Jahn, A.; Aksenov, Y.; deCuevas, B. A.; deSteur, L.; Haekkinen, S.; Hansen, E.; Herbaut, C.; Houssais, M.-N.; Karcher, M.; Kauker, F.;

Agglutinated Foraminifera indicate a deep bottom current over the Hovgaard Ridge, West of Spitsbergen

NASA Astrophysics Data System (ADS)

Kaminski, Michael; Frank, Niessen

2015-04-01

The Hovgård Ridge is situated in Fram Strait, west of Spitsbergen. The ridge either represents a submerged fragment of continental crust or an upwarped fragmant of ocean crust within the Fram Strait. Its crest rises to a water depth of approx. 1170 m. During Expedition 87 of the Icebreaker POLARSTERN in August 2014, a sediment-echosounding profile was recorded and a boxcore station was collected from the crest of Hovgård Ridge at 1169 m water depth. The surficial sediment at this station consists of dark yellowish brown pebbly-sandy mud with a minor admixture of biogenic components in the coarse fraction. Patches of large tubular foraminifera and isolated pebbles were clearly visible on the sediment surface. The sediment surface of the boxcore was covered with patches of large (>1 mm diameter) large tubular astrorhizids belonging mostly to the species Astrorhiza crassatina Brady, with smaller numbers of Saccorhiza, Hyperammina, and Psammosiphonella. Non-tubular species consist mainly of opportunistic forms such as Psammosphaera and Reophax. The presence of large suspension-feeding tubular genera as well as opportunistic forms, as well as sediment winnowing, point to the presence of a deep current at this locality that is strong enough to disturb the benthic fauna. This is confirmed by data obtained from sediment echosounding, which exhibit lateral variation of relative sedimentation rates within the Pleistocene sedimentary drape covering the ridge indicative of winnowing in a south-easterly direction.

Operation of a Hovercraft Scientific Platform Over Sea Ice in the Arctic Ocean Transpolar Drift (81 - 85N): The FRAM-2012 Experience

NASA Astrophysics Data System (ADS)

Hall, J. K.; Kristoffersen, Y.

2013-12-01

We have tested the feasibility of hovercraft travel through predominantly first year ice of the Transpolar Drift between 81°N - 85°N north of Svalbard. With 2-9 ridges per kilometer, our hovercraft (Griffon TD2000 Mark II), with an effective hover height of about 0.5 m, had to travel a distance 1.3 times the great circle distance between the point of origin and the final destination. Instantaneous speeds were mostly 5-7 knots. Two weeks later icebreaker Oden completed the same transit under conditions with no significant pressure in the ice at a speed mostly 1 knot higher than the hovercraft and travelled 1.2 times the great circle distance. The hovercraft spent 25 days monitoring micro-earthquake activity of the Arctic Mid-Ocean Ridge at a section of the spreading center where no seismicity has been recorded by the global seismograph network. More than ten small earthquake events per day were recorded. Visibility appears to be the most critical factor to hovercraft travel in polar pack ice. Improved control of hovercraft motion would substantially increase the potential usefulness of hovercraft in the sea ice environment. University of Bergen graduate student Gaute Hope emplacing one of the hydrophones in the triangular array used to locate small earthquakes over the Gakkel Ridge rift valley around 85N during FRAM-2012. The research hovercraft R/H SABVABAA is in the background.

Risk-seeking for losses is associated with 5-HTTLPR, but not with transient changes in 5-HT levels.

PubMed

Neukam, Philipp T; Kroemer, Nils B; Deza Araujo, Yacila I; Hellrung, Lydia; Pooseh, Shakoor; Rietschel, Marcella; Witt, Stephanie H; Schwarzenbolz, Uwe; Henle, Thomas; Smolka, Michael N

2018-05-05

Serotonin (5-HT) plays a key role in different aspects of value-based decision-making. A recent framework proposed that tonic 5-HT (together with dopamine, DA) codes future average reward expectations, providing a baseline against which possible choice outcomes are compared to guide decision-making. To test whether high 5-HT levels decrease loss aversion, risk-seeking for gains, and risk-seeking for losses. In a first session, 611 participants were genotyped for 5-HTTLPR and performed a mixed gambles (MGA) task and two probability discounting tasks for gains and losses, respectively (PDG/PDL). Afterwards, a subsample of 105 participants (44 with S/S, 6 with S/L, 55 with L/L genotype) completed the pharmacological study using a crossover design with tryptophan depletion (ATD), loading (ATL), and balanced (BAL) conditions. The same decision constructs were assessed. We found increased risk-seeking for losses in S/S compared to L/L individuals at the first visit (p = 0.002). Neither tryptophan depletion nor loading affected decision-making, nor did we observe an interaction between intervention and 5-HTTLPR genotype. Our data do not support the idea that transient changes of tonic 5-HT affect value-based decision-making. We provide evidence for an association of 5-HTTLPR with risk-seeking for losses, independent of acute 5-HT levels. This indicates that the association of 5-HTTLPR and risk-seeking for losses is mediated via other mechanisms, possibly by differences in the structural development of neural circuits of the 5-HT system during early life phases.

Hydrogen leak detection in the Space Shuttle

NASA Technical Reports Server (NTRS)

Barile, Ronald G

1992-01-01

This study focuses on a helium gas jet flowing into room air. Measurements of helium concentration and velocity in the jet-air mixture are reported. The objective is to learn about jet characteristics so that dynamically similar hydrogen leaks may be located in the Space Shuttle. The hazardous gas detection system (HGDS) in the mobile launch pad uses mass spectrometers to monitor the shuttle environment for leaks. The mass spectrometers are fed by long sample tubes which draw gas from the payload bay, mid body, aft engine compartment and external tank. The overall purpose of this study is to improve the HGDS especially in its potential for locating hydrogen leaks. A rapid-response leak detection experiment was designed, built, and tested, following on the work done in this program last summer. The apparatus included a Perkin Elmer MGA-1200 mass spectrometer and air velocity transducer, both monitored by a Macintosh IIFX computer using LabVIEW software. A jet of helium flowing into the lab air simulated a gas leak. Steady helium or hydrogen-nitrogen jets were logged for concentration and velocity, and the power spectral density of each was computed. Last year, large eddies and vortices were visually seen with Schlieren imaging, and they were detected in the time plots of the various instruments. The response time of the MGA-1200 was found in the range of 0.05 to 0.1 sec. Pulsed concentration waves were clearly detected at 25 cycles per sec by spectral analysis of MGA data. No peaks were detected in the power spectrum, so in the present study, 10 Hz bandwidth-averaged power levels were examined at regular frequency intervals. The practical consequences of last year's study are as follows: sampling frequency should be increased above the present rate of 1 sample per second so that transients could be observed and analyzed with frequency response methods. Many more experiments and conditions were observed in this second summer, including the effects of orifice diameter, jet velocity, sample tube design, radial effects, vertical flow, and low hydrogen concentrations (1 percent). A frequent observation was that the power spectrum, calculated from the Fourier transform of concentration fluctuations, gives a separate piece of information from concentration. Many of the tests suggest that power is high where mixing occurs at the helium-air interface. This fact is apparently independent of the concentration level, which could be high or low, but depends on the sample location relative to the jet (leak) origin, whereas high concentration may be due to a strong leak far away or a small leak close to the sample tube. If the power is low for any concentration level, this would signify helium is arriving at the sample tube by diffusion, not chaotic mixing caused by the jet interaction with air. The practical result is to propose a modification of the HGDL mass spectrometer data sampling and software so that sampling rates could be capable of observing at least 25 Hz fluctuations.

OPALS: A COTS-based Tech Demo of Optical Communications

NASA Technical Reports Server (NTRS)

Oaida, Bogdan

2012-01-01

I. Objective: Deliver video from ISS to optical ground terminal via an optical communications link. a) JPL Phaeton/Early Career Hire (ECH) training project. b) Implemented as Class-D payload. c) Downlink at approx.30Mb/s. II. Flight System a) Optical Head Beacon Acquisition Camera. Downlink Transmitter. 2-axis Gimbal. b) Sealed Container Laser Avionics Power distribution Digital I/O board III. Implementation: a) Ground Station - Optical Communications Telescope Laboratory at Table Mountain Facility b) Flight System mounted to ISS FRAM as standard I/F. Attached externally on Express Logistics Carrier.

Unmanned Aircraft Systems For CryoSat-2 Validation

NASA Astrophysics Data System (ADS)

Crocker, Roger Ian; Maslanik, James A.

2011-02-01

A suite of sensors has been assembled to map surface elevation with fine-resolution from small unmanned aircraft systems (UAS). The sensor package consists of a light detecting and ranging (LIDAR) instrument, an inertial measurement unit (IMU), a GPS module, and digital still and video cameras. It has been utilized to map ice sheet topography in Greenland and to measure sea ice freeboard and roughness in Fram Strait. Data collected during these campaigns illustrate its potential to compliment ongoing CryoSat-2 (CS-2) calibration and validation efforts.

Opportunity's Travels

NASA Technical Reports Server (NTRS)

2004-01-01

This overview map made from Mars Orbiter camera images illustrates the path that the Mars Exploration Rover Opportunity has taken from its first sol on the red planet through its 87th sol. After thoroughly examining its 'Eagle Crater' landing-site, the rover moved onto the plains of Meridiani Planum, stopping to examine a curious trough and a target within it called 'Anatolia.' Following that, Opportunity approached and remotely studied the rocky dish called 'Fram Crater.' As of its 91st sol (April 26, 2004), the rover sits 160 meters (about 525 feet) from the rim of 'Endurance Crater.'

Nonvolatile Memory Technology for Space Applications

NASA Technical Reports Server (NTRS)

Oldham, Timothy R.; Irom, Farokh; Friendlich, Mark; Nguyen, Duc; Kim, Hak; Berg, Melanie; LaBel, Kenneth A.

2010-01-01

This slide presentation reviews several forms of nonvolatile memory for use in space applications. The intent is to: (1) Determine inherent radiation tolerance and sensitivities, (2) Identify challenges for future radiation hardening efforts, (3) Investigate new failure modes and effects, and technology modeling programs. Testing includes total dose, single event (proton, laser, heavy ion), and proton damage (where appropriate). Test vehicles are expected to be a variety of non-volatile memory devices as available including Flash (NAND and NOR), Charge Trap, Nanocrystal Flash, Magnetic Memory (MRAM), Phase Change--Chalcogenide, (CRAM), Ferroelectric (FRAM), CNT, and Resistive RAM.

IE Data Processing.

DTIC Science & Technology

1984-10-01

RN4 DMT P6 CAnQ fram P4 Satellite ID-343567656469B Sensor Bias Node Voltages 1 1.27 0. 2 7.54 6.31 3 16.42 15.26 4 28.93 27.87 NmDber of sectors per...Monitor Interoretation Event Yloitor Voltage Change Sensor Operation Mode Cange Duration Frequency 0 20 Elec: Density Cal2 1024 ion: Density 1 K 2, 3of...34"Event monitor voltage decreases linearly fron 500 to 100 during electron sweep. • Event monitor voltage increases linearly from 10 to 500 during

Purification of the Alpha Glycerophosphate Oxidase from African Trypanosomes

DTIC Science & Technology

1988-02-02

remo~val of all unattached enzymes , the GPO would be eluted with a buffer containing the substrate a- GP. Cyancqen bromide activated Sepharose 4B was...released upon eluticn with a buffer cm-xtaining 100 rm of a-GP. Unfortunately, in these experiments, trypan blue was eluted from the colmn and the enzyme ...borate buffer (pH 8.0), there was no leakage of the ligand but the GPO was not released fram the colun when eluted with 10aM c-GP. Enzyme assays of the

Thiosulfate as an Antidote to Mustard Poisoning, A Review of the Literature.

DTIC Science & Technology

1982-09-01

FRANCISCO CA UNCLASSIFIED K D MCKINLEY ET AL. SEP 82 LRIR-127 F/G 61209 N MENOMONEE NONIEhhhhhhhhhEND 1168 UL 1.0. * 1.25 I 1,1.6 MICROCOPY RESOLUTION TEST...ORGANIZATION NAME AND ADDRESS 10. PRORA ELEMENT. PROJECT, TASK AREA &J’WORC UNIT N UMSIERS Division of Research Support, Letterman ArmyPojN/3124A7 Institute...block 2,If different fram Report) I& SUPPLI111ENTARY NOTES I9. KEY WORDS (Centinue n reverse side If natessy and identlity5 b ock number) Sodium

Multiple slope failures shaped the lower continental slope offshore NW Svalbard in the Fram Strait

NASA Astrophysics Data System (ADS)

Osti, Giacomo; Mienert, Jürgen; Forwick, Matthias; Sverre Laberg, Jan

2016-04-01

Bathymetry data show that the lower slope (between 1300 m and 3000 m water depth) of the NW-Svalbard passive margin has been affected by multiple slope failure events. The single events differ in terms of extension, volume of mobilized sediments, morphology of the slide scar, run-out distance and age. As for several mega-scale and minor Arctic slides, the trigger mechanism is still speculative and may include high sedimentation rates, dissociation of gas hydrates, excess pore pressure, or earthquakes caused by isostatic rebound. In this study, we discuss the potential trigger mechanisms that have led to the multiple slope failure events within what we suggest to be named the Fram Strait Slide Complex. The slide complex lies in proximity to the tectonically active Spitsbergen Fracture Zone where earthquakes events, occurrences of potential weak layers in the sediment column, low sedimentation rates, and extended gas hydrate-bearing sediments may all have contributed to the causes leading to multiple slope failures. Preliminary results obtained from 14C dating on N. pachyderma sin. from sediment cores from the Spitsbergen Fracture Zone slides (SFZS 1 and 2), coupled with sub-bottom profiler data (frequency 9 to 15 KHz) show that the two shallowest glide planes within one of the observed slide scars failed ~100,000 and ~115,000 yr BP. Whilst SFZS 1 affected an area of 750 km2 mobilizing a total sediment volume of 40 km3, SFZS 2 moved an area of 230 km2 with a sediment volume of 4.5 km3.

Variations in freshwater pathways from the Arctic Ocean into the North Atlantic Ocean

NASA Astrophysics Data System (ADS)

Wang, Zeliang; Hamilton, James; Su, Jie

2017-06-01

Understanding the mechanisms that drive exchanges between the Arctic Ocean and adjacent oceans is critical to building our knowledge of how the Arctic is reacting to a warming climate, and how potential changes in Arctic Ocean freshwater export may impact the AMOC (Atlantic Meridional Overturning Circulation). Here, freshwater pathways from the Arctic Ocean to the North Atlantic are investigated using a 1 degree global model. An EOF analysis of modeled sea surface height (SSH) demonstrates that while the second mode accounts for only 15% of the variability, the associated geostrophic currents are strongly correlated with freshwater exports through CAA (Canadian Arctic Archipelago; r = 0.75), Nares Strait (r = 0.77) and Fram Strait (r = -0.60). Separation of sea level into contributing parts allows us to show that the EOF1 is primarily a barotropic mode reflecting variability in bottom pressure equivalent sea level, while the EOF2 mode reflects changes in steric height in the Arctic Basin. This second mode is linked to momentum wind driven surface current, and dominates the Arctic Ocean freshwater exports. Both the Arctic Oscillation and Arctic Dipole atmospheric indices are shown to be linked to Arctic Ocean freshwater exports, with the forcing associated with the Arctic Dipole reflecting the out-of-phase relationship between transports through the CAA and those through Fram Strait. Finally, observed freshwater transport variation through the CAA is found to be strongly correlated with tide gauge data from the Beaufort Sea coast (r = 0.81), and with the EOF2 mode of GRACE bottom pressure data (r = 0.85) on inter-annual timescales.

Microzooplankton abundance, composition and trophic interactions with phytoplankton and pelagic copepods in the ice-covered and open waters of the Eastern Fram Strait.

NASA Astrophysics Data System (ADS)

Franzè, G.; Lavrentyev, P.; Svensen, C.; Moore, F. B.

2016-02-01

Microzooplankton distribution and trophodynamics were examined in the Fram Strait in May and September 2014 as part of the Carbon Bridge multidisciplinary program focused on the biological effects of the Atlantic water transfer to the Arctic Ocean. Samples of microzooplankton were collected along three longitudinal transects from the slope to the ice edge and crossed the Western Spitzbergen Current (WSC). Integrated microzooplankton biomass in the upper 50 m ranged from 0.19 mg C m-2 above the slope to 3.22 mg C m-2 within WSC. Mixotrophic oligotrich ciliates from the genus Strombidium comprised most of the biomass and reached their subsurface maximum of 78,000 cells L-1 and 206 µg C L-1 in the Atlantic water. This is the highest volumetric biomass of microzooplankton reported from the polar seas so far. Large heterotrophic dinoflagellates Gyrodinium and Protoperidinium were also abundant at several stations. Microzooplankton herbivory rates measured in shipboard dilution experiments ranged from non-detectable within the bloom of Phaeocystis pouchetii to >100% of phytoplankton growth. Overall, the herbivory and production rates were nearly matched and increased from 0.2 d-1 in May to > 1.0 d-1 in September. The Atlantic copepod Calanus finmarchicus showed a clear preference for ciliates under the ice and obtained nearly 80% of its carbon-based diet from microzooplankton. These preliminary results support the idea that the microbial food web plays a central role in the marine carbon cycle in the Arctic.

Seasonal and Interannual Variations of Sea Ice Mass Balance From the Central Arctic to the Greenland Sea

NASA Astrophysics Data System (ADS)

Lei, Ruibo; Cheng, Bin; Heil, Petra; Vihma, Timo; Wang, Jia; Ji, Qing; Zhang, Zhanhai

2018-04-01

The seasonal evolution of sea ice mass balance between the Central Arctic and Fram Strait, as well as the underlying driving forces, remain largely unknown because of a lack of observations. In this study, two and three buoys were deployed in the Central Arctic during the summers of 2010 and 2012, respectively. It was established that basal ice growth commenced between mid-October and early December. Annual basal ice growth, ranging from 0.21 to 1.14 m, was determined mainly by initial ice thickness, air temperature, and oceanic heat flux during winter. An analytic thermodynamic model indicated that climate warming reduces the winter growth rate of thin ice more than for thick ice because of the weak thermal inertia of the former. Oceanic heat flux during the freezing season was 2-4 W m-2, which accounted for 18-31% of the basal ice energy balance. We identified two mechanisms that modified the oceanic heat flux, i.e., solar energy absorbed by the upper ocean during summer, and interaction with warm waters south of Fram Strait; the latter resulted in basal ice melt, even in winter. In summer 2010, ice loss in the Central Arctic was considerable, which led to increased oceanic heat flux into winter and delayed ice growth. The Transpolar Drift Stream was relatively weak in summer 2013. This reduced sea ice advection out of the Arctic Ocean, and it restrained ice melt because of the cool atmospheric conditions, weakened albedo feedback, and relatively small oceanic heat flux in the north.

Arctic sea ice thickness characteristics in winter 2004 and 2007 from submarine sonar transects

NASA Astrophysics Data System (ADS)

Wadhams, Peter; Hughes, Nick; Rodrigues, JoãO.

2011-08-01

A transect of the Arctic Ocean by the British submarine Tireless in March 2007 enabled the thickness characteristics of the ice cover to be measured during the winter immediately preceding the exceptional retreat of summer 2007. In this paper we report on mean and modal drafts, probability density functions of draft, and the frequency and depth distribution of pressure ridges, and we compare results with those from an earlier submarine cruise in winter 2004 which covered part of the same area. In the region from north of Fram Strait to Ellesmere Island (about 85°N, 0-70°W) we find no change in mean drafts between 2004 and 2007 though there is a change in ice composition, with more ridging in 2007 but a lesser modal draft. This agrees with the observations of younger ice being driven toward Fram Strait in 2007. The region north of Ellesmere Island continues to be a "redoubt" containing more thick deformed multiyear ice than any other part of the transect. In the west the submarine profiled extensively under the SEDNA ice camp at 73°N 145°W. This is in the same location as the 1976 AIDJEX ice camp and a sonar survey done by a U.S. submarine in April 1976. We found that a large decrease in mean draft had occurred (32%) over 31 years and that in 2007 the SEDNA region contained the thinnest ice of any part of the Arctic surveyed by the submarine; this was a region from which the ice completely retreated during the subsequent summer of 2007.

The missing "Bratz" doll: a case of vaginal foreign body.

PubMed

Someshwar, Jean; Lutfi, Riad; Nield, Linda S

2007-12-01

Young female patients with gynecologic complaints, including vaginal discharge, commonly seek care in the emergency department. Prepubertal vaginal discharges have various potential etiologies including nonspecific vulvovaginitis, infections, inflammatory or dermatologic processes, precocious puberty, malignancy, anatomical anomaly, trauma, sexual abuse, and foreign body. We present the case of a 4-year-old girl with vaginal odor and discharge secondary to a foreign body. The foreign body was part of a toy, the lower half of the patient's missing "Bratz" (MGA Entertainment) doll. Although it is well known that a vaginal foreign body can cause a vaginal discharge, the unusual nature of the foreign body in this case and the revealing radiograph made this a case of particular interest.

Similar on the Inside (pre-grinding)

NASA Technical Reports Server (NTRS)

2004-01-01

This approximate true-color image taken by the panoramic camera on the Mars Exploration Rover Opportunity show the rock called 'Pilbara' located in the small crater dubbed 'Fram.' The rock appears to be dotted with the same 'blueberries,' or spherules, found at 'Eagle Crater.' Spirit drilled into this rock with its rock abrasion tool. After analyzing the hole with the rover's scientific instruments, scientists concluded that Pilbara has a similar chemical make-up, and thus watery past, to rocks studied at Eagle Crater. This image was taken with the panoramic camera's 480-, 530- and 600-nanometer filters.

Similar on the Inside (post-grinding)

NASA Technical Reports Server (NTRS)

2004-01-01

This approximate true-color image taken by the panoramic camera on the Mars Exploration Rover Opportunity show the hole drilled into the rock called 'Pilbara,' which is located in the small crater dubbed 'Fram.' Spirit drilled into this rock with its rock abrasion tool. The rock appears to be dotted with the same 'blueberries,' or spherules, found at 'Eagle Crater.' After analyzing the hole with the rover's scientific instruments, scientists concluded that Pilbara has a similar chemical make-up, and thus watery past, to rocks studied at Eagle Crater. This image was taken with the panoramic camera's 480-, 530- and 600-nanometer filters.

MIZEX: A Program for Mesoscale Air-Ice-Ocean Interaction Experiments in Arctic Marginal Ice Zones. VIII. A Science Plan for a Winter Marginal Ice Zone Experiment in the Fram Strait/Greenland Sea: 1987/89,

DTIC Science & Technology

1986-04-01

forward modeling, with the pa- be telemetered via the ARGOS system for real - rameter changes needed to bring the predictions time evaluation, and the...integrated en ’i- rtinnental measurement svs fern. quisition system to the Winter MIZEX in I-ram To control and direct the experiment, real - time Strait...to measure, under- Electromagnetic sensing via aircraft and satellites stand, and model: will be employed in real time to identify eddy " Changes in

Impacts of Biomass Burning on Organic Aerosols over the Northwestern Pacific Ocean

NASA Astrophysics Data System (ADS)

Guo, T.; Guo, Z.

2017-12-01

During the cruise from East China Sea to Northwestern Pacific in March-April 2014, total suspended particle samples were collected and analyzed for tracers of primary and secondary organic aerosols (SOA) as well as OC and EC. In the study, the sum of all tracers during the sampling period ranged from 3.60 to 181.58 ng/m3, with a mean being 59.87±62.70 ng/m3. Among these tracers, glucose was the dominant compound (average: 17.73±20.60 ng/m3), followed by levoglucosan (12.82±14.37 ng/m3) and fructose (10.47±13.28 ng/m3). LEVO in samples affected by long range transport of biomass burning aerosol (17.38±21.32ng/m3) was about 1 order magnitude higher than the other (1.76±0.92ng/m3, p<0.05), which showed the long rang transport of biomass burning aerosols would indeed affect the aerosol over NWPO. Among these SOA tracers, Isoprene SOA tracers were the dominant compound, followed by Monoterpene, Aromatic and Sesquiterpene tracers. The high-NOx product, 2-methylglyceric acid (MGA; average: 3.82±5.99 ng/m3) accounted for more than half of the total amount of the isoprene tracers, followed by low-NOx products, 2-methyltetrols (2.71±4.58 ng/m3) and C5-alkene diols (0.31±0.73 ng/m3). This MGA-majority pattern could be explained by the high-NOx conditions caused by forest fires, with further evidences given by 3-day BTs. Thus organic aerosols over NWPO were deeply influenced by forest fires taking place in Siberia and North China as a result of long-range transport of both directly emitted OA and secondarily formed OA under high-NOx conditions during fire events.

On the Evolution of Handedness: Evidence for Feeding Biases

PubMed Central

Flindall, Jason W.; Gonzalez, Claudia L. R.

2013-01-01

Many theories have been put forward to explain the origins of right-handedness in humans. Here we present evidence that this preference may stem in part from a right hand advantage in grasping for feeding. Thirteen participants were asked to reach-to-grasp food items of 3 different sizes: SMALL (Cheerios®), MEDIUM (Froot Loops®), and LARGE (Oatmeal Squares®). Participants used both their right- and left-hands in separate blocks (50 trials each, starting order counterbalanced) to grasp the items. After each grasp, participants either a) ate the food item, or b) placed it inside a bib worn beneath his/her chin (25 trials each, blocked design, counterbalanced). The conditions were designed such that the outward and inward movement trajectories were similar, differing only in the final step of placing it in the mouth or bib. Participants wore Plato liquid crystal goggles that blocked vision between trials. All trials were conducted in closed-loop with 5000 ms of vision. Hand kinematics were recorded by an Optotrak Certus, which tracked the position of three infrared diodes attached separately to the index finger, thumb, and wrist. We found a task (EAT/PLACE) by hand (LEFT/RIGHT) interaction on maximum grip aperture (MGA; the maximum distance between the index finger and thumb achieved during grasp pre-shaping). MGAs were smaller during right-handed movements, but only when grasping with intent to eat. Follow-up tests show that the RIGHT-HAND/EAT MGA was significantly smaller than all other hand/task conditions. Because smaller grip apertures are typically associated with greater precision, our results demonstrate a right-hand advantage for the grasp-to-eat movement. From an evolutionary perspective, early humans may have preferred the hand that could grasp food with more precision, thereby maximizing the likelihood of retrieval, consumption, and consequently, survival. PMID:24236078

MGA trajectory planning with an ACO-inspired algorithm

NASA Astrophysics Data System (ADS)

Ceriotti, Matteo; Vasile, Massimiliano

2010-11-01

Given a set of celestial bodies, the problem of finding an optimal sequence of swing-bys, deep space manoeuvres (DSM) and transfer arcs connecting the elements of the set is combinatorial in nature. The number of possible paths grows exponentially with the number of celestial bodies. Therefore, the design of an optimal multiple gravity assist (MGA) trajectory is a NP-hard mixed combinatorial-continuous problem. Its automated solution would greatly improve the design of future space missions, allowing the assessment of a large number of alternative mission options in a short time. This work proposes to formulate the complete automated design of a multiple gravity assist trajectory as an autonomous planning and scheduling problem. The resulting scheduled plan will provide the optimal planetary sequence and a good estimation of the set of associated optimal trajectories. The trajectory model consists of a sequence of celestial bodies connected by two-dimensional transfer arcs containing one DSM. For each transfer arc, the position of the planet and the spacecraft, at the time of arrival, are matched by varying the pericentre of the preceding swing-by, or the magnitude of the launch excess velocity, for the first arc. For each departure date, this model generates a full tree of possible transfers from the departure to the destination planet. Each leaf of the tree represents a planetary encounter and a possible way to reach that planet. An algorithm inspired by ant colony optimization (ACO) is devised to explore the space of possible plans. The ants explore the tree from departure to destination adding one node at the time: every time an ant is at a node, a probability function is used to select a feasible direction. This approach to automatic trajectory planning is applied to the design of optimal transfers to Saturn and among the Galilean moons of Jupiter. Solutions are compared to those found through more traditional genetic-algorithm techniques.

Metal ion displacements in noncentrosymmetric chalcogenides La3Ga1.67S7, La3Ag0.6GaCh7 (Ch=S, Se), and La3MGaSe7 (M=Zn, Cd)

NASA Astrophysics Data System (ADS)

Iyer, Abishek K.; Yin, Wenlong; Rudyk, Brent W.; Lin, Xinsong; Nilges, Tom; Mar, Arthur

2016-11-01

The quaternary Ga-containing chalcogenides La3Ag0.6GaS7, La3Ag0.6GaSe7, La3ZnGaSe7, and La3CdGaSe7, as well as the related ternary chalcogenide La3Ga1.67S7, were prepared by reactions of the elements at 950 °C. They adopt noncentrosymmetric hexagonal structures (space group P63, Z=2) with cell parameters (a=10.2 Å, c=6.1 Å for the sulfides; a=10.6 Å, c=6.4 Å for the selenides) that are largely controlled by the geometrical requirements of one-dimensional stacks of Ga-centered tetrahedra separated by the La atoms. Among these compounds, which share the common formulation La3M1-xGaCh7 (M=Ga, Ag, Zn, Cd; Ch=S, Se), the M atoms occupy sites within a stacking of trigonal antiprisms formed by Ch atoms. The location of the M site varies between extremes with trigonal antiprismatic (CN6) and trigonal planar (CN3) geometry. Partial occupation of these sites and intermediate ones accounts for the considerable versatility of these structures and the occurrence of large metal displacement parameters. The site occupations can be understood in a simple way as being driven by the need to satisfy appropriate bond valence sums for both the M and Ch atoms. Band structure calculations rationalize the substoichiometry observed in the Ag-containing compounds (La3Ag0.6GaS7, La3Ag0.6GaSe7) as a response to overbonding. X-ray photoelectron spectroscopy supports the presence of monovalent Ag atoms in these compounds, which are not charge-balanced.

Overexpression of Wild-Type Aspartokinase Increases l-Lysine Production in the Thermotolerant Methylotrophic Bacterium Bacillus methanolicus▿

PubMed Central

Jakobsen, Øyvind M.; Brautaset, Trygve; Degnes, Kristin F.; Heggeset, Tonje M. B.; Balzer, Simone; Flickinger, Michael C.; Valla, Svein; Ellingsen, Trond E.

2009-01-01

Aspartokinase (AK) controls the carbon flow into the aspartate pathway for the biosynthesis of the amino acids l-methionine, l-threonine, l-isoleucine, and l-lysine. We report here the cloning of four genes (asd, encoding aspartate semialdehyde dehydrogenase; dapA, encoding dihydrodipicolinate synthase; dapG, encoding AKI; and yclM, encoding AKIII) of the aspartate pathway in Bacillus methanolicus MGA3. Together with the known AKII gene lysC, dapG and yclM form a set of three AK genes in this organism. Overexpression of dapG, lysC, and yclM increased l-lysine production in wild-type B. methanolicus strain MGA3 2-, 10-, and 60-fold (corresponding to 11 g/liter), respectively, without negatively affecting the specific growth rate. The production levels of l-methionine (less than 0.5 g/liter) and l-threonine (less than 0.1 g/liter) were low in all recombinant strains. The AK proteins were purified, and biochemical analyses demonstrated that they have similar Vmax values (between 47 and 58 μmol/min/mg protein) and Km values for l-aspartate (between 1.9 and 5.0 mM). AKI and AKII were allosterically inhibited by meso-diaminopimelate (50% inhibitory concentration [IC50], 0.1 mM) and by l-lysine (IC50, 0.3 mM), respectively. AKIII was inhibited by l-threonine (IC50, 4 mM) and by l-lysine (IC50, 5 mM), and this enzyme was synergistically inhibited in the presence of both of these amino acids at low concentrations. The correlation between the impact on l-lysine production in vivo and the biochemical properties in vitro of the individual AK proteins is discussed. This is the first example of improving l-lysine production by metabolic engineering of B. methanolicus and also the first documentation of considerably increasing l-lysine production by overexpression of a wild-type AK. PMID:19060158

Overexpression of wild-type aspartokinase increases L-lysine production in the thermotolerant methylotrophic bacterium Bacillus methanolicus.

PubMed

Jakobsen, Oyvind M; Brautaset, Trygve; Degnes, Kristin F; Heggeset, Tonje M B; Balzer, Simone; Flickinger, Michael C; Valla, Svein; Ellingsen, Trond E

2009-02-01

Aspartokinase (AK) controls the carbon flow into the aspartate pathway for the biosynthesis of the amino acids l-methionine, l-threonine, l-isoleucine, and l-lysine. We report here the cloning of four genes (asd, encoding aspartate semialdehyde dehydrogenase; dapA, encoding dihydrodipicolinate synthase; dapG, encoding AKI; and yclM, encoding AKIII) of the aspartate pathway in Bacillus methanolicus MGA3. Together with the known AKII gene lysC, dapG and yclM form a set of three AK genes in this organism. Overexpression of dapG, lysC, and yclM increased l-lysine production in wild-type B. methanolicus strain MGA3 2-, 10-, and 60-fold (corresponding to 11 g/liter), respectively, without negatively affecting the specific growth rate. The production levels of l-methionine (less than 0.5 g/liter) and l-threonine (less than 0.1 g/liter) were low in all recombinant strains. The AK proteins were purified, and biochemical analyses demonstrated that they have similar V(max) values (between 47 and 58 micromol/min/mg protein) and K(m) values for l-aspartate (between 1.9 and 5.0 mM). AKI and AKII were allosterically inhibited by meso-diaminopimelate (50% inhibitory concentration [IC(50)], 0.1 mM) and by l-lysine (IC(50), 0.3 mM), respectively. AKIII was inhibited by l-threonine (IC(50), 4 mM) and by l-lysine (IC(50), 5 mM), and this enzyme was synergistically inhibited in the presence of both of these amino acids at low concentrations. The correlation between the impact on l-lysine production in vivo and the biochemical properties in vitro of the individual AK proteins is discussed. This is the first example of improving l-lysine production by metabolic engineering of B. methanolicus and also the first documentation of considerably increasing l-lysine production by overexpression of a wild-type AK.

Storm-driven Mixing and Potential Impact on the Arctic Ocean

NASA Technical Reports Server (NTRS)

Yang, Jiayan; Comiso, Josefino; Walsh, David; Krishfield, Richard; Honjo, Susumu; Koblinsky, Chester J. (Technical Monitor)

2001-01-01

Observations of the ocean, atmosphere, and ice made by Ice-Ocean Environmental Buoys (IOEBs) indicate that mixing events reaching the depth of the halocline have occurred in various regions in the Arctic Ocean. Our analysis suggests that these mixing events were mechanically forced by intense storms moving across the buoy sites. In this study, we analyzed these mixing events in the context of storm developments that occurred in the Beaufort Sea and in the general area just north of Fram Strait, two areas with quite different hydrographic structures. The Beaufort Sea is strongly influenced by inflow of Pacific water through Bering Strait, while the area north of Fram Strait is directly affected by the inflow of warm and salty North Atlantic water. Our analyses of the basin-wide evolution of the surface pressure and geostrophic wind fields indicate that the characteristics of the storms could be very different. The buoy-observed mixing occurred only in the spring and winter seasons when the stratification was relatively weak. This indicates the importance of stratification, although the mixing itself was mechanically driven. We also analyze the distribution of storms, both the long-term climatology as well as the patterns for each year in the last two decades. The frequency of storms is also shown to be correlated- (but not strongly) to Arctic Oscillation indices. This study indicates that the formation of new ice that leads to brine rejection is unlikely the mechanism that results in the type of mixing that could overturn the halocline. On the other hand, synoptic-scale storms can force mixing deep enough to the halocline and thermocline layer. Despite a very stable stratification associated with the Arctic halocline, the warm subsurface thermocline water is not always insulated from the mixed layer.

Characterization of a turbomolecular-pumped magnetic sector mass spectrometer

NASA Technical Reports Server (NTRS)

Mehta, Narinder K.

1988-01-01

A Perkin Elmer MGA-1200, turbomolecular-pumped, magnetic sector, multiple gas analyzer mass spectrometer with modified inlet for fast response was characterized for the analysis of hydrogen, helium, oxygen and argon in nitrogen and helium background gases. This instrument was specially modified for the Vanderberg AFB SLC-6 Hydrogen Disposal Test Program, as a part of the Hydrogen Sampling System (H2S2). Linearity, precision, drift, detection limits and accuracy among other analytical parameters for each of the background gas were studied to evaluate the performance of the instrument. The result demonstrates that H2S2 mass spectrometer is a stable instrument and can be utilized for the quantitative analytical determination of hydrogen, helium, oxygen and argon in nitrogen and helium background gases.

China Macroeconomic: Documentation Data Banks. Volume 3. Foreign Trade.

DTIC Science & Technology

1984-11-15

1983 data from Chlnese "Cmtan, Statistics," Econmic Infomation and Agency, THoE ong Pr ious years from CIA "International Trade Statistics" Load fram...204,097 145,564 515,062 1984 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 6 Sources, see below 19F, 1983 data from Chinese "Customs Statistics," Econmic ...Statistics," EconmIc Information and Agency, F org Previous years fran CIA "Internationl Trade Statistics" Load from rw 12 to row 54 -.96 - A B C D E F G H

Fridtjof Nansen - Scientist, Diplomat and Humanist

NASA Astrophysics Data System (ADS)

Gjelsvik, Tore

In my opinion, no Norwegian has made a deeper impression upon his contemporaries than Fridtjof Nansen. Even today, more than 60 years after he passed away, young people who want to make an extraordinary physical effort talk about walking in his footsteps - or skiing in his ski tracks. References to Nansen's humanitarian efforts after the first world war can be read almost daily in Norwegian newspapers in articles discussing aid to refugees, starving populations or ethnic minorities threatened with extermination. More than 200,000 people, many of them foreigners, visit Nansen's famous polar ship FRAM, housed on the peninsula Bygdøy, on the outskirt of Oslo.

Sea Ice, Climate and Fram Strait

NASA Technical Reports Server (NTRS)

Hunkins, K.

1984-01-01

When sea ice is formed the albedo of the ocean surface increases from its open water value of about 0.1 to a value as high as 0.8. This albedo change effects the radiation balance and thus has the potential to alter climate. Sea ice also partially seals off the ocean from the atmosphere, reducing the exchange of gases such as carbon dioxide. This is another possible mechanism by which climate might be affected. The Marginal Ice Zone Experiment (MIZEX 83 to 84) is an international, multidisciplinary study of processes controlling the edge of the ice pack in that area including the interactions between sea, air and ice.

On the fate of laser-produced NH 2 in a constrained pulsed expansion of trimethylamine alane and NH 3

NASA Astrophysics Data System (ADS)

Demchuk, Alexander; Cahill, John J.; Simpson, Steven; Koplitz, Brent

2001-11-01

The effects of both 193 nm radiation and NH 3 on an expansion of trimethylamine alane (TMAA) have been studied. In neat TMAA, 193 nm radiation induces small but significant clustering. When NH 3 is introduced in the absence of 193 nm photons, no visible reaction occurs. However, when NH 3 is introduced along with 193 nm photons, the H 3Al:N(CH 3) 3 parent molecule is completely replaced by H 3AlNH 2. Moreover, the clustering channels observed with neat TMAA disappear. The apparent stability or inertness of the R 3MNH 2 (R=H, CH 3 or C 2H 5; M=Ga or Al) species in a variety of metal nitride reactive environments is discussed.

[Rhein promotes the expression of SIRT1 in kidney tissues of type 2 diabetic rat].

PubMed

Chen, Weidong; Chang, Baochao; Zhang, Yan; Yang, Ping; Liu, Lei

2015-05-01

To observe the effect of rhein on the expression of SIRT1(Sirtuin 1) in kidney of diabetic rats, and to explore the role of rhein in protecting rat kidney against diabetic nephropathy and possible mechanism. The type 2 diabetic rats were induced by high-glucose and high-fat diet combined with streptozotocin (35 mg/kg body mass). Seventy-five eight-week-old male SD rats were randomly divided into 6 groups: normal group, diabetic group, low-, medium- and high-dose (50, 100, 150 mg/kg) rhein treatment groups and 10 mg/kg pioglitazone treatment group. The rats were given corresponding substances intragastrically once a day. At the end of the 16th week, the fasting plasma glucose (FPG), fasting insulin (FINS), triglycerides (TG), total cholesterol (TC), serum creatinine (Scr) and 24 hours urine protein (24 h U-PRO) were determined. The renal hypertrophy index (KM/BM), insulin resistance index (HOMA-IR) were calculated. The pathological changes in renal tissues were examined by PAS staining under a light microscopy. The mean glomerular area (MGA) and mean glomerular volume (MGV) were measured by pathological image analysis system. Western blotting and real-time quantitative PCR were used to determine the expression of SIRT1 in renal tissues at protein and mRNA levels, respectively. The expression of SIRT1 was down-regulated in the kidney of diabetic rats. The levels of FPG, FINS, HOMA-IR, TG, TC, Scr, 24 h U-PRO, KM/BM, MGA and MGV significantly decreased and the histopathology of renal tissues were significantly improved in all treatment groups compared with diabetic group. The expression of SIRT1 mRNA and protein markedly increased in rhein treatment groups and pioglitazone treatment group compared with diabetic group. The indicators in high-dose rhein treatment group were improved more significantly than those in the other groups. Correlation analysis showed that the expression of SIRT1 was negatively correlated with 24 h U-PRO and MGV. The expression of SIRT1 was reduced in kidney tissues of diabetic rats. Rhein could attenuate kidney damage in diabetic rats by improving the insulin resistance and dyslipidemia, and increasing the SIRT1 expression.

The Streptococcus pyogenes serotype M49 Nra-Ralp3 transcriptional regulatory network and its control of virulence factor expression from the novel eno ralp3 epf sagA pathogenicity region.

PubMed

Kreikemeyer, Bernd; Nakata, Masanobu; Köller, Thomas; Hildisch, Hendrikje; Kourakos, Vassilios; Standar, Kerstin; Kawabata, Shigetada; Glocker, Michael O; Podbielski, Andreas

2007-12-01

Many Streptococcus pyogenes (group A streptococcus [GAS]) virulence factor- and transcriptional regulator-encoding genes cluster together in discrete genomic regions. Nra is a central regulator of the FCT region. Previous studies exclusively described Nra as a transcriptional repressor of adhesin and toxin genes. Here transcriptome and proteome analysis of a serotype M49 GAS strain and an isogenic Nra mutant of this strain revealed the complete Nra regulon profile. Nra is active in all growth phases tested, with the largest regulon in the transition phase. Almost exclusively, virulence factor-encoding genes are repressed by Nra; these genes include the GAS pilus operon, the capsule synthesis operon, the cytolysin-mediated translocation system genes, all Mga region core virulence genes, and genes encoding other regulators, like the Ihk/Irr system, Rgg, and two additional RofA-like protein family regulators. Surprisingly, our experiments revealed that Nra additionally acts as a positive regulator, mostly for genes encoding proteins and enzymes with metabolic functions. Epidemiological investigations revealed strong genetic linkage of one particular Nra-repressed regulator, Ralp3 (SPy0735), with a gene encoding Epf (extracellular protein factor from Streptococcus suis). In a serotype-specific fashion, this ralp3 epf gene block is integrated, most likely via transposition, into the eno sagA virulence gene block, which is present in all GAS serotypes. In GAS serotypes M1, M4, M12, M28, and M49 this novel discrete genetic region is therefore designated the eno ralp3 epf sagA (ERES) pathogenicity region. Functional experiments showed that Epf is a novel GAS plasminogen-binding protein and revealed that Ralp3 activity counteracts Nra and MsmR regulatory activity. In addition to the Mga and FCT regions, the ERES region is the third discrete chromosomal pathogenicity region. All of these regions are transcriptionally linked, adding another level of complexity to the known GAS growth phase-dependent regulatory network.

The Streptococcus pyogenes Serotype M49 Nra-Ralp3 Transcriptional Regulatory Network and Its Control of Virulence Factor Expression from the Novel eno ralp3 epf sagA Pathogenicity Region▿ †

PubMed Central

Kreikemeyer, Bernd; Nakata, Masanobu; Köller, Thomas; Hildisch, Hendrikje; Kourakos, Vassilios; Standar, Kerstin; Kawabata, Shigetada; Glocker, Michael O.; Podbielski, Andreas

2007-01-01

Many Streptococcus pyogenes (group A streptococcus [GAS]) virulence factor- and transcriptional regulator-encoding genes cluster together in discrete genomic regions. Nra is a central regulator of the FCT region. Previous studies exclusively described Nra as a transcriptional repressor of adhesin and toxin genes. Here transcriptome and proteome analysis of a serotype M49 GAS strain and an isogenic Nra mutant of this strain revealed the complete Nra regulon profile. Nra is active in all growth phases tested, with the largest regulon in the transition phase. Almost exclusively, virulence factor-encoding genes are repressed by Nra; these genes include the GAS pilus operon, the capsule synthesis operon, the cytolysin-mediated translocation system genes, all Mga region core virulence genes, and genes encoding other regulators, like the Ihk/Irr system, Rgg, and two additional RofA-like protein family regulators. Surprisingly, our experiments revealed that Nra additionally acts as a positive regulator, mostly for genes encoding proteins and enzymes with metabolic functions. Epidemiological investigations revealed strong genetic linkage of one particular Nra-repressed regulator, Ralp3 (SPy0735), with a gene encoding Epf (extracellular protein factor from Streptococcus suis). In a serotype-specific fashion, this ralp3 epf gene block is integrated, most likely via transposition, into the eno sagA virulence gene block, which is present in all GAS serotypes. In GAS serotypes M1, M4, M12, M28, and M49 this novel discrete genetic region is therefore designated the eno ralp3 epf sagA (ERES) pathogenicity region. Functional experiments showed that Epf is a novel GAS plasminogen-binding protein and revealed that Ralp3 activity counteracts Nra and MsmR regulatory activity. In addition to the Mga and FCT regions, the ERES region is the third discrete chromosomal pathogenicity region. All of these regions are transcriptionally linked, adding another level of complexity to the known GAS growth phase-dependent regulatory network. PMID:17893125

Screening for Vulnerability in Older Cancer Patients: The ONCODAGE Prospective Multicenter Cohort Study

PubMed Central

Soubeyran, Pierre; Bellera, Carine; Goyard, Jean; Heitz, Damien; Curé, Hervé; Rousselot, Hubert; Albrand, Gilles; Servent, Véronique; Jean, Olivier Saint; van Praagh, Isabelle; Kurtz, Jean-Emmanuel; Périn, Stéphane; Verhaeghe, Jean-Luc; Terret, Catherine; Desauw, Christophe; Girre, Véronique; Mertens, Cécile; Mathoulin-Pélissier, Simone; Rainfray, Muriel

2014-01-01

Background Geriatric Assessment is an appropriate method for identifying older cancer patients at risk of life-threatening events during therapy. Yet, it is underused in practice, mainly because it is time- and resource-consuming. This study aims to identify the best screening tool to identify older cancer patients requiring geriatric assessment by comparing the performance of two short assessment tools the G8 and the Vulnerable Elders Survey (VES-13). Patients and Methods The diagnostic accuracy of the G8 and the (VES-13) were evaluated in a prospective cohort study of 1674 cancer patients accrued before treatment in 23 health care facilities. 1435 were eligible and evaluable. Outcome measures were multidimensional geriatric assessment (MGA), sensitivity (primary), specificity, negative and positive predictive values and likelihood ratios of the G8 and VES-13, and predictive factors of 1-year survival rate. Results Patient median age was 78.2 years (70-98) with a majority of females (69.8%), various types of cancer including 53.9% breast, and 75.8% Performance Status 0-1. Impaired MGA, G8, and VES-13 were 80.2%, 68.4%, and 60.2%, respectively. Mean time to complete G8 or VES-13 was about five minutes. Reproducibility of the two questionnaires was good. G8 appeared more sensitive (76.5% versus 68.7%, P =  0.0046) whereas VES-13 was more specific (74.3% versus 64.4%, P<0.0001). Abnormal G8 score (HR = 2.72), advanced stage (HR = 3.30), male sex (HR = 2.69) and poor Performance Status (HR = 3.28) were independent prognostic factors of 1-year survival. Conclusion With good sensitivity and independent prognostic value on 1-year survival, the G8 questionnaire is currently one of the best screening tools available to identify older cancer patients requiring geriatric assessment, and we believe it should be implemented broadly in daily practice. Continuous research efforts should be pursued to refine the selection process of older cancer patients before potentially life-threatening therapy. PMID:25503576

Teleseismic P-wave Delay Time Tomography of the southern Superior Province and Midcontinent Rift System (MRS) Region

NASA Astrophysics Data System (ADS)

Bollmann, T. A.; van der Lee, S.; Frederiksen, A. W.; Wolin, E.; Aleqabi, G. I.; Revenaugh, J.; Wiens, D. A.; Darbyshire, F. A.

2014-12-01

The Superior Province Rifting Earthscope Experiment (SPREE) and the northern midwest footprint of USArray's Transportable Array recorded continuous ground motion for a period of 2.5 years. From around 400 M>5.5 teleseismic earthquakes recorded at 337 stations, we measured body wave delay times for 255 of these earthquakes. The P wave delays are accumulated over more than 45 thousand wave paths with turning points in the lower mantle. We combine these delay times with a similar number delay times used in previous tomographic studies of the study region. The latter delay times stem from fewer stations, including Polaris and CNSN stations, and nearly a thousand earthquakes. We combine these two sets of delay times to image the three-dimensional distribution of seismic velocity variations beneath the southern Superior Province and surrounding provinces. This combined data coverage is illustrated in the accompanying figure for a total number of 447 stations . The coverage and the combined delays form the best configuration yet to image the three-dimensional distribution of seismic P and S-wave velocity variations beneath the southern Superior and surrounding provinces. Closely spaced stations (~12 km) along and across the MRS provide higher resolving power for lithospheric structure beneath the rift system. Conforming to expectations that the entire region is underlain by thick, cool lithosphere, a mean delay of -.55 +/- .54 s. This is very similar to the mean delays -.6s +/- .37s measured for this region before 2012. Event corrections range from -.2 +/-.54 s and correlate with tectonics for 80% of the earthquakes. An inversion of these nearly one hundred thousand P and around thirty thousand S-wave delay times for high-resolution P and S-wave velocity structure, respectively, does not show structures that are obviously related to the crustal signature of the MRS. None of structures imaged, align with or have a similar shape to the high Mid-continent Gravity Anomaly (MGA). However, a low-velocity structure is imaged in the lithosphere just east of the MGA.

Generation of Volatile Organic Compounds from Dissolved Organic Matter in far North Atlantic Surface Ocean Waters.

NASA Astrophysics Data System (ADS)

Hudson, E. D.; Ariya, P. A.

2005-12-01

The photochemical degradation of dissolved organic matter (DOM) in surface ocean waters is thought to be a source of volatile organic compounds (VOC) (including non-methane hydrocarbons and low MW carbonyl compounds) to the remote marine troposphere. We report on the characterization of DOM sampled at over 30 sites in the far North Atlantic (Greenland and Norwegian seas, Fram strait) during the summer of 2004, and on experiments to identify factors responsible for the photochemical generation of VOCs in these samples. The results will be discussed in the context of VOC profiles of whole air samples taken to match the seawater samples in time and space.

Opportunity's Travels During its First 205 Martian Days

NASA Technical Reports Server (NTRS)

2004-01-01

This map shows the traverse of NASA's Mars Exploration Rover Opportunity through the rover's 205th martian day, or sol (Aug. 21, 2004). The background image is from the rover's descent imaging camera. Images inset along the route are from Opportunity's navigation camera. Opportunity began its exploration inside 'Eagle' crater near the left edge of the map. Following completion of its study of the outcrop there, it traversed eastward to a small crater ('Fram' crater) before driving southeastward to the rim of 'Endurance' crater. After a survey partly around the south rim of Endurance crater, Opportunity drove inside the southwest rim of Endurance crater and began a systematic study of outcrops exposed on the crater's inner slope.

Continued Development and Implementation of the Universal Network Interface Device (UNID) II in the Digital Engineering Laboratory Network (DELNET). Volume 2.

DTIC Science & Technology

1984-12-01

PACKET LO-ATED IN TABLE LCNTTB. INPUT < THE INDUT IS A TWO CHARACTER ASCII VALUE INDICATING THE TABLE REQUIRING EVALUATION . > PROCESSING THIS PROCEDURE...INTO OHANYE A (2). I NPUT THE INPUT IS AN INTEGER VA UE INDICATING’ THE TAlN’LE LOCATI’ OF THE FRAmE TO BE EVALUATED . PROC--SSING THIS PROCEDURE LOU-S AT...GOOD AEXICdLFCL ~N IS NuT rC:L I VED LY THE END OF THE WAIT PER ICOH, I r I S fx>Sit-..) ACAI N UPION IriL NE.XT CflLL THRJOH THIS IRROCEDURE. A [ RAMC OF

Remote sensing of the marginal ice zone during Marginal Ice Zone Experiment (MIZEX) 83

NASA Technical Reports Server (NTRS)

Shuchman, R. A.; Campbell, W. J.; Burns, B. A.; Ellingsen, E.; Farrelly, B. A.; Gloersen, P.; Grenfell, T. C.; Hollinger, J.; Horn, D.; Johannessen, J. A.

1984-01-01

The remote sensing techniques utilized in the Marginal Ice Zone Experiment (MIZEX) to study the physical characteristics and geophysical processes of the Fram Strait Region of the Greenland Sea are described. The studies, which utilized satellites, aircraft, helicopters, and ship and ground-based remote sensors, focused on the use of microwave remote sensors. Results indicate that remote sensors can provide marginal ice zone characteristics which include ice edge and ice boundary locations, ice types and concentration, ice deformation, ice kinematics, gravity waves and swell (in the water and the ice), location of internal wave fields, location of eddies and current boundaries, surface currents and sea surface winds.

Differential Scanning Calorimetry and Evolved Gas Analysis at Mars Ambient Conditions Using the Thermal Evolved Gas Analyzer (TEGA)

NASA Technical Reports Server (NTRS)

Musselwhite, D. S.; Boynton, W. V.; Ming, Douglas W.; Quadlander, G.; Kerry, K. E.; Bode, R. C.; Bailey, S. H.; Ward, M. G.; Pathare, A. V.; Lorenz, R. D.

2000-01-01

Differential Scanning Calorimetry (DSC) combined with evolved gas analysis (EGA) is a well developed technique for the analysis of a wide variety of sample types with broad application in material and soil sciences. However, the use of the technique for samples under conditions of pressure and temperature as found on other planets is one of current C development and cutting edge research. The Thermal Evolved Gas Analyzer (MGA), which was designed, built and tested at the University of Arizona's Lunar and Planetary Lab (LPL), utilizes DSC/EGA. TEGA, which was sent to Mars on the ill-fated Mars Polar Lander, was to be the first application of DSC/EGA on the surface of Mars as well as the first direct measurement of the volatile-bearing mineralogy in martian soil.

The Life-Cycle of a Polar Low Observed with Research Aircraft Dropsondes

NASA Astrophysics Data System (ADS)

Saetra, O.; Linders, T.

2008-12-01

The structure and life cycle of a polar low that formed over the ocean south of the Fram Strait on 3 and 4 March 2008 is described using GPS dropsondes from the DLR Falcon research aircraft and by AVHRR satellite images. Data from three successive flights during the life cycle of the polar low will be presented. The Norwegian IPY-THORPEX project carried out a field experiment between February and May 2008, aiming at investigating adverse weather in the Arctic. During the campaign period, the DLR Falcon aircraft was stationed at Andoya Airport in Northern Norway. On the evening 2 March the synoptic scale flow triggered a cold-air outbreak in the Fram Strait and the western Barents Sea. The weather analysis from the Norwegian weather service (met.no)indicated strong reversed shear flow (warm air to the left) and favorable conditions for the formations of polar lows. A flight for the next morning was planned. At the time of departure AVHRR satellite images of deep-convective clouds revealed the presence of an Arctic front in the northerly winds between Greenland and the Svalbard islands. During this flight 18 dropsondes were successfully released. A second flight was carried out later the same day, 3 March. At this time a meso-scale cyclone had formed at the southern tip of the Arctic Front. The cyclone was measured in three cross-sections with 14 successful sondes released. The night between 3 and 4 March the cyclone intensified and by the third flight a mature polar low was located outside the coast of Trondelag in Norway. The last flight was carried out, staring on the morning 4 March, making 4 cross-sections with 20 dropsondes. On the evening 4 March, shortly after the third flight, the polar low made landfall and faded rapidly.

Bathymetric patterns in standing stock and diversity of deep-sea nematodes at the long-term ecological research observatory HAUSGARTEN (Fram Strait)

NASA Astrophysics Data System (ADS)

Grzelak, Katarzyna; Kotwicki, Lech; Hasemann, Christiane; Soltwedel, Thomas

2017-08-01

Bathymetric patterns in standing stocks and diversity are a major topic of investigation in deep-sea biology. From the literature, responses of metazoan meiofauna and nematodes to bathymetric gradients are well studied, with a general decrease in biomass and abundance with increasing water depth, while bathymetric diversity gradients often, although it is not a rule, show a unimodal pattern. Spatial distribution patterns of nematode communities along bathymetric gradients are coupled with surface-water processes and interacting physical and biological factors within the benthic system. We studied the nematode communities at the Long-Term Ecological Research (LTER) observatory HAUSGARTEN, located in the Fram Strait at the Marginal Ice Zone, with respect to their standing stocks as well as structural and functional diversity. We evaluated whether nematode density, biomass and diversity indices, such as H0, Hinf, EG(50), Θ- 1, are linked with environmental conditions along a bathymetric transect spanning from 1200 m to 5500 m water depth. Nematode abundance, biomass and diversity, as well as food availability from phytodetritus sedimentation (indicated by chloroplastic pigments in the sediments), were higher at the stations located at upper bathyal depths (1200-2000 m) and tended to decrease with increasing water depth. A faunal shift was found below 3500 m water depth, where genus composition and trophic structure changed significantly and structural diversity indices markedly decreased. A strong dominance of very few genera and its high turnover particularly at the abyssal stations (4000-5500 m) suggests that environmental conditions were rather unfavorable for most genera. Despite the high concentrations of sediment-bound chloroplastic pigments and elevated standing stocks found at the deepest station (5500 m), nematode genus diversity remained the lowest compared to all other stations. This study provides a further insight into the knowledge of deep-sea nematodes, their diversity patterns and a deeper understanding of the environmental factors shaping nematodes communities at bathyal and abyssal depths.

Exploring Science Through Polar Exploration

NASA Astrophysics Data System (ADS)

Pfirman, S. L.; Bell, R. E.; Zadoff, L.; Kelsey, R.

2003-12-01

Exploring the Poles is a First Year Seminar course taught at Barnard College, Columbia University. First Year Seminars are required of incoming students and are designed to encourage critical analysis in a small class setting with focused discussion. The class links historical polar exploration with current research in order to: introduce non-scientists to the value of environmental science through polar literature; discuss issues related to venturing into the unknown that are of relevance to any discipline: self-reliance, leadership, preparation, decisions under uncertainty; show students the human face of science; change attitudes about science and scientists; use data to engage students in exploring/understanding the environment and help them learn to draw conclusions from data; integrate research and education. These goals are met by bringing analysis of early exploration efforts together with a modern understanding of the polar environment. To date to class has followed the efforts of Nansen in the Fram, Scott and Amundsen in their race to the pole, and Shackleton's Endurance. As students read turn-of-the-century expedition journals, expedition progress is progressively revealed on an interactive map showing the environmental context. To bring the exploration process to life, students are assigned to expedition teams for specific years and the fates of the student "expeditions" are based on their own decisions. For example, in the Arctic, they navigate coastal sea ice and become frozen into the ice north of Siberia, re-creating Nansen's polar drift. Fates of the teams varied tremendously: some safely emerged at Fram Strait in 4 years, while others nearly became hopelessly lost in the Beaufort Gyre. Students thus learn about variability in the current polar environment through first hand experience, enabling them to appreciate the experiences, decisions, and, in some cases, the luck, of polar explorers. Evaluation by the Columbia Center for New Media, Teaching and Learning shows that combining historical texts with current data/simulations is an extremely powerful way of engaging non-scientists in science, and explaining the role of science and the environment in decision-making.

The Holocene Record of the Arctic Oscillation and a Possible Link to Solar Variability

NASA Astrophysics Data System (ADS)

Rand, J.; Darby, D. A.; Ortiz, J.; Cook, M. Y.; Keigwin, L.

2007-12-01

Detailed Fe grain provenance for a 19.9 meter long piston core (HLY02-JPC16) with about 17.5 m of Holocene sediment provides a sub-century scale resolution of the Arctic Oscillation (AO). The presence of Fe grains matched to Russian shelves fluctuates throughout this core, located 125 km north of Alaska in 1300 m water depth. High amounts of these Fe grains indicate a strong positive AO. While century scale fluctuations occur in the influx of Russian ice-rafted grains at this core site, a significant periodicity of about 1500 years exists that is similar to that of Be-10 in the Greenland ice cores. This begs the question as to how the AO might be linked to solar variations, especially such weak ones. The sources of sea ice rafting throughout the Holocene are compared to Modern sea ice samples and there is generally a good match. There is much greater heterogeneity in sources based on Fe grain provenance than other techniques for sourcing sea ice today. While the Laptev Sea is certainly an important sea ice entrainment area, it is by no means the only one and not even the most important over the long term. This distinction lies with northern Canadian sources, especially the Queen Elizabeth Islands facing the Arctic Ocean. The AO plays a major role in mixing sea ice from Russian and North American sources, especially during ++AO events. At these times, not only does the Trans Polar Drift swing closer to North America introducing sea ice from the Russian shelves such as the Laptev Sea to the Beaufort Gyre (BG), but it also aids in dragging some of the BG ice toward Fram Strait. This BG ice is a mix of North American and Russian ice and thus it is not surprising to find sediment from both sources in ice along the drift path of this ice moving toward Fram Strait.

Cenozoic pre-glacial tectonostratigraphy and erosion estimates for the northwestern Barents Sea

NASA Astrophysics Data System (ADS)

Lasabuda, Amando; Sverre Laberg, Jan; Knutsen, Stig-Morten

2017-04-01

The northwestern Barents Sea continental margin is located between Bjørnøya and Svalbard. It is a structurally complex area characterized by a series of highs and basins influenced by: 1) the formation of the Spitsbergen fold-and-thrust belt towards the north and the pull-apart basin, the Vestbakken Volcanic Province, to the south, and 2) the rifting and opening of the Fram Strait, the deep-water gateway connecting the Norwegian - Greenland Sea and the Arctic Ocean. This study incorporate newly available 2D seismic data as well as magnetic data, and aim to improve the understanding of the Cenozoic evolution of this area, including better constrain of the timing of the main sedimentation events of the Cenozoic basins and estimates of the volume of sediments involved and the corresponding rates of erosion of the drainage area. The Cenozoic development of this area is strongly related to the rifting and opening of the Norwegian-Greenland Sea. During the Paleocene-Eocene, the northwestern Barents Sea margin were subjected to compression/transpression when Greenland drifted towards Svalbard that led to uplift and the development of fold-and-thrust belt on Svalbard. Subsequently, from the Oligocene, a tectonic plate reorganization occurred, leading to crustal extension, sea floor spreading and opening of the Fram Strait west of Svalbard. The seismic data shows a pronounced sequence of Early - Mid Cenozoic, pre-glacial sediments overlying the oceanic crust west of Svalbard while to the east, the Svalbard platform and the Stappen High were subjected to erosion and probably acted as the main sediment source for the northwestern Barents Sea margin. The amount of erosion will be estimated from the study of the deposited sediment volumes and their inferred source area. We will then compare the sedimentation and erosion rates to rates from other parts of the Norwegian - Barents Sea - Svalbard margin as well as relevant modern systems. Furthermore, the Cenozoic paleo-geography will be reconstructed.

LA-ICP-MS as Tool for Provenance Analyses in Arctic Marine Sediments

NASA Astrophysics Data System (ADS)

Wildau, Antje; Garbe-Schönberg, Dieter

2015-04-01

The hydraulic transport of sediments is a major geological process in terrestrial and marine systems and is responsible for the loss, redistribution and accumulation of minerals. Provenance analyses are a powerful tool for assessing the origin and dispersion of material in ancient and modern fluvial and marine sediments. Provenance-specific heavy minerals (e.g., zircon, rutile, tourmaline) can therefore be used to provide valuable information on the formation of ore deposits (placer deposits), and the reconstruction of paleogeography, hydrology, climate conditions and developments. The application of provenances analyses for the latter reason is of specific interest, since there is need for research on the progressing climate change, and heavy minerals represent good proxies for the evaluation of recent and past changes in the climate. The study of these fine particles provides information about potential regional or long distance transport paths, glacial / ice drift and current flows, freezing and melting events as well as depositional centers for the released sediments. Classic methods applied for provenance analyses are mapping of the presence / absence of diagnostic minerals, their grain size distribution, modal mineralogy and the analysis of variations in ratio of two or more heavy minerals. Electron microprobe has been established to discover changes in mineral chemistry of individual mineral phases, which can indicate fluctuations or differences in the provenance. All these methods bear the potential of high errors that lower the validity of the provenance analyses. These are for example the misclassification of mineral species due to undistinguishable optical properties or the limitations in the detection / variations of trace elements using the election microprobe. For this case study, marine sediments from the Arctic Ocean have been selected to test if LA-ICP-MS can be established as a key technique for precise and reliable provenance analyses. The Laptev Sea is known to be a "sea ice formation factory" and represents a perfect source area with numerous sediment loaded rivers draining into the Arctic Ocean. Mineral grains become trapped in the sea ice, which is transported to the Fram Strait, the outflow area of the Transpolar Drift System. Thus, minerals in the Fram Strait and in the Laptev Sea should have the same provenance. In both areas zircon, garnet, ilmenite, magnetite, tourmaline, pyroxene and amphibole were identified (amongst others). The vast majority of potential source areas and the widespread occurrence of these accessory and rock forming minerals result in the absolute need for a highly sensitive and precise method such as LA-ICP-MS. We report new data on the eligibility of selected heavy minerals for provenance analyses in the Arctic Ocean. Based on the individual trace element composition, REE-pattern and isotopic ratios, reflecting the conditions during formation, we report individual fingerprints for single mineral species. This enables us to allocate specific minerals from Fram Strait and from Laptev Sea to one provenance. Furthermore we evaluate the eligibility of different heavy minerals as a geochemical proxy in Arctic sediments for provenance analyses using LA-ICP-MS.

Bottom melting of Arctic Sea Ice in the Nansen Basin due to Atlantic Water influence

NASA Astrophysics Data System (ADS)

Muilwijk, Morven; Smedsrud, Lars H.; Meyer, Amelie

2016-04-01

Our global climate is warming, and a shrinking Arctic sea ice cover remains one of the most visible signs of this warming. Sea Ice loss is now visible for all months in all regions of the Arctic. Hydrographic and current observations from a region north of Svalbard collected during the Norwegian Young Sea Ice Cruise (N-ICE2015) are presented here. Comparison with historical data shows that the new observations from January through June fill major gaps in available observations, and help describing important processes linking changes in regional Atlantic Water (AW) heat transport and sea ice. Warm and salty AW originating in the North Atlantic enters the Arctic Ocean through the Fram Strait and is present below the Arctic Sea Ice cover throughout the Arctic. However, the depth of AW varies by region and over time. In the region north of Svalbard, we assume that depth could be governed primarily by local processes, by upstream conditions of the ice cover (Northwards), or by upstream conditions of the AW (Southwards). AW carries heat corresponding to the volume transport of approximately 9 SV through Fram Strait, varying seasonally from 28 TW in winter to 46 TW in summer. Some heat is recirculated, but the net annual heat flux into the Arctic Ocean from AW is estimated to be around 40 TW. The Atlantic Water layer temperature at intermediate depths (150-900m) has increased in recent years. Until recently, maximum temperatures have been found to be 2-3 C in the Nansen Basin. Studies have shown that for example, in the West Spitsbergen Current the upper 50-200m shows an overall AW warming of 1.1 C since 1979. In general we expect efficient melting when AW is close to the surface. Previously the AW entering through Fram Strait has been considered as less important because changes in the sea ice cover have been connected to greater inflow of Pacific Water through Bering Strait and atmospheric forcing. Conversely it is now suggested that AW has direct impact on melting of sea ice. Because of the large increase in AW temperature over the last 30 years we assume that perturbations in the AW are important drivers of location of AW in this region, and that the sea ice and polar water above is passively responding to the AW variability. Previously it has been argued that the warming of AW could not contribute to increased ice melting because of the strong stratification. Our observations show an ice cover around 2 m, but with active ice formation in between the larger and thicker floes. The ongoing freezing drives brine release and subsequent convection, contributing to the deep ~100 m mixed layer observed in the area until mid-May. Onwards from May solar heating is stratifying the upper layer by adding heat. Data analysis is ongoing but indicates that location of AW is an important factor in bottom melting in the area north of Svalbard. Location of AW and related bottom melting will be evaluated using simulations from a fully coupled climate model.

Arctic Ocean circulation during the anoxic Eocene Azolla event

NASA Astrophysics Data System (ADS)

Speelman, Eveline; Sinninghe Damsté, Jaap; März, Christian; Brumsack, Hans; Reichart, Gert-Jan

2010-05-01

The Azolla interval, as encountered in Eocene sediments from the Arctic Ocean, is characterized by organic rich sediments ( 4wt% Corg). In general, high levels of organic matter may be caused by increased productivity, i.e. extensive growth of Azolla, and/or enhanced preservation of organic matter, or a combination of both. Anoxic (bottom) water conditions, expanded oxygen minimum zones, or increased sedimentation rates all potentially increase organic matter preservation. According to plate tectonic, bathymetric, and paleogeographic reconstructions, the Arctic Ocean was a virtually isolated shallow basin, with one possible deeper connection to the Nordic Seas represented by a still shallow Fram Strait (Jakobsson et al., 2007), hampering ventilation of the Arctic Basin. During the Azolla interval surface waters freshened, while at the same time bottom waters appear to have remained saline, indicating that the Arctic was highly stratified. The restricted ventilation and stratification in concert with ongoing export of organic matter most likely resulted in the development of anoxic conditions in the lower part of the water column. Whereas the excess precipitation over evaporation maintained the freshwater lid, sustained input of Nordic Sea water is needed to keep the deeper waters saline. To which degree the Arctic Ocean exchanged with the Nordic Seas is, however, still largely unknown. Here we present a high-resolution trace metal record (ICP-MS and ICP-OES) for the expanded Early/Middle Eocene section capturing the Azolla interval from Integrated Ocean Drilling Program (IODP) Expedition 302 (ACEX) drilled on the Lomonosov Ridge, central Arctic Ocean. Euxinic conditions throughout the interval resulted in the efficient removal of redox sensitive trace metals from the water column. Using the sedimentary trace metal record we also constrained circulation in the Arctic Ocean by assessing the relative importance of trace metal input sources (i.e. fluvial, eolian, and through seawater inflow). Excess vanadium accumulation during the Azolla event (80 ppm), basin volume and surface area, average vanadium sea (1.8 ppb) and river water (1.0 ppb) concentrations, together indicate that an inflow of Nordic Sea water of 0.2 Sv is needed to sustain vanadium levels. The same calculation using molybdenum gives an inflow of only 0.02 Sv. These low inflow rates imply Arctic Ocean (deep) water residence times of 2000 - 20000 years, respectively. Based on climate modeling we calculated a summed net amount of precipitation for the Eocene Arctic Basin (Precipitation - Evaporation + Runoff) of 0.46 Sv. Together these notions indicate that a compensating inflow of saline North Atlantic water occurred, accompanied by an outflow of more fresh waters, resulting in a bi-directional, two-layer flow through the (proto-) Fram Strait. Consequently, the limited exchange of water through the Fram Strait implies that a relatively low export productivity would have been sufficient to render Arctic bottom waters anoxic. Jakobsson, M., Backman, J., Rudels, B., Nycander, J., Frank, M., Mayer, L., Jokat, W., Sangiorgi, F., O'Regan, M., Brinkhuis, H., King, J., Moran, K. (2007). The early Miocene onset of a ventilated circulation regimen in the Arctic Ocean. Nature 447, 986-990.

Evolution of microwave sea ice signatures during early summer and midsummer in the marginal ice zone

NASA Technical Reports Server (NTRS)

Onstott, R. G.; Grenfell, T. C.; Matzler, C.; Luther, C. A.; Svendsen, E. A.

1987-01-01

Emissivities at frequencies from 5 to 94 GHz and backscatter at frequencies from 1 to 17 GHz were measured from sea ice in Fram Strait during the marginal Ice Zone Experiment in June and July of 1983 and 1984. The ice observed was primarily multiyear; the remainder, first-year ice, was often deformed. Results from this active and passive microwave study include the description of the evolution of the sea ice during early summer and midsummer; the absorption properties of summer snow; the interrelationship between ice thickness and the state and thickness of snow; and the modulation of the microwave signature, especially at the highest frequencies, by the freezing of the upper few centimeters of the ice.

Conversion-Integration of MSFC Nonlinear Signal Diagnostic Analysis Algorithms for Realtime Execution of MSFC's MPP Prototype System

NASA Technical Reports Server (NTRS)

Jong, Jen-Yi

1996-01-01

NASA's advanced propulsion system Small Scale Magnetic Disturbances/Advanced Technology Development (SSME/ATD) has been undergoing extensive flight certification and developmental testing, which involves large numbers of health monitoring measurements. To enhance engine safety and reliability, detailed analysis and evaluation of the measurement signals are mandatory to assess its dynamic characteristics and operational condition. Efficient and reliable signal detection techniques will reduce the risk of catastrophic system failures and expedite the evaluation of both flight and ground test data, and thereby reduce launch turn-around time. During the development of SSME, ASRI participated in the research and development of several advanced non- linear signal diagnostic methods for health monitoring and failure prediction in turbomachinery components. However, due to the intensive computational requirement associated with such advanced analysis tasks, current SSME dynamic data analysis and diagnostic evaluation is performed off-line following flight or ground test with a typical diagnostic turnaround time of one to two days. The objective of MSFC's MPP Prototype System is to eliminate such 'diagnostic lag time' by achieving signal processing and analysis in real-time. Such an on-line diagnostic system can provide sufficient lead time to initiate corrective action and also to enable efficient scheduling of inspection, maintenance and repair activities. The major objective of this project was to convert and implement a number of advanced nonlinear diagnostic DSP algorithms in a format consistent with that required for integration into the Vanderbilt Multigraph Architecture (MGA) Model Based Programming environment. This effort will allow the real-time execution of these algorithms using the MSFC MPP Prototype System. ASRI has completed the software conversion and integration of a sequence of nonlinear signal analysis techniques specified in the SOW for real-time execution on MSFC's MPP Prototype. This report documents and summarizes the results of the contract tasks; provides the complete computer source code; including all FORTRAN/C Utilities; and all other utilities/supporting software libraries that are required for operation.

Edge enhancement and noise suppression for infrared image based on feature analysis

NASA Astrophysics Data System (ADS)

Jiang, Meng

2018-06-01

Infrared images are often suffering from background noise, blurred edges, few details and low signal-to-noise ratios. To improve infrared image quality, it is essential to suppress noise and enhance edges simultaneously. To realize it in this paper, we propose a novel algorithm based on feature analysis in shearlet domain. Firstly, as one of multi-scale geometric analysis (MGA), we introduce the theory and superiority of shearlet transform. Secondly, after analyzing the defects of traditional thresholding technique to suppress noise, we propose a novel feature extraction distinguishing image structures from noise well and use it to improve the traditional thresholding technique. Thirdly, with computing the correlations between neighboring shearlet coefficients, the feature attribute maps identifying the weak detail and strong edges are completed to improve the generalized unsharped masking (GUM). At last, experiment results with infrared images captured in different scenes demonstrate that the proposed algorithm suppresses noise efficiently and enhances image edges adaptively.

Development and Attestation of Gamma-Ray Measurement Methodologies for use by Rostekhnadzor Inspectors in the Russian Federation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jeff Sanders

2006-09-01

Development and attestation of gamma-ray non-destructive assay measurement methodologies for use by inspectors of the Russian Federal Service for Environmental, Technological, and Nuclear Oversight (Rostekhnadzor, formerly Gosatomnadzor or GAN), as well as for use by Russian nuclear facilities, has been completed. Specifically, a methodology utilizing the gamma-ray multi group analysis (MGA) method for determining plutonium isotopic composition has been developed, while existing methodologies to determining uranium enrichment and isotopic composition have been revised to make them more appropriate to the material types and conditions present in nuclear facilities in the Russian Federation. This paper will discuss the development and revisionmore » of these methodologies, the metrological characteristics of the final methodologies, as well as the limitations and concerns specific to the utilization of these analysis methods in the Russian Federation.« less

Assessing the Robustness of Complete Bacterial Genome Segmentations

NASA Astrophysics Data System (ADS)

Devillers, Hugo; Chiapello, Hélène; Schbath, Sophie; El Karoui, Meriem

Comparison of closely related bacterial genomes has revealed the presence of highly conserved sequences forming a "backbone" that is interrupted by numerous, less conserved, DNA fragments. Segmentation of bacterial genomes into backbone and variable regions is particularly useful to investigate bacterial genome evolution. Several software tools have been designed to compare complete bacterial chromosomes and a few online databases store pre-computed genome comparisons. However, very few statistical methods are available to evaluate the reliability of these software tools and to compare the results obtained with them. To fill this gap, we have developed two local scores to measure the robustness of bacterial genome segmentations. Our method uses a simulation procedure based on random perturbations of the compared genomes. The scores presented in this paper are simple to implement and our results show that they allow to discriminate easily between robust and non-robust bacterial genome segmentations when using aligners such as MAUVE and MGA.

Localized 5f electrons in superconducting PuCoIn₅: consequences for superconductivity in PuCoGa₅.

PubMed

Bauer, E D; Altarawneh, M M; Tobash, P H; Gofryk, K; Ayala-Valenzuela, O E; Mitchell, J N; McDonald, R D; Mielke, C H; Ronning, F; Griveau, J-C; Colineau, E; Eloirdi, R; Caciuffo, R; Scott, B L; Janka, O; Kauzlarich, S M; Thompson, J D

2012-02-08

The physical properties of the first In analog of the PuMGa(5) (M = Co, Rh) family of superconductors, PuCoIn(5), are reported. With its unit cell volume being 28% larger than that of PuCoGa(5), the characteristic spin-fluctuation energy scale of PuCoIn(5) is three to four times smaller than that of PuCoGa(5), which suggests that the Pu 5f electrons are in a more localized state relative to PuCoGa(5). This raises the possibility that the high superconducting transition temperature T(c) = 18.5 K of PuCoGa(5) stems from the proximity to a valence instability, while the superconductivity at T(c) = 2.5 K of PuCoIn(5) is mediated by antiferromagnetic spin fluctuations associated with a quantum critical point.

Modified Mahalanobis Taguchi System for Imbalance Data Classification

PubMed Central

2017-01-01

The Mahalanobis Taguchi System (MTS) is considered one of the most promising binary classification algorithms to handle imbalance data. Unfortunately, MTS lacks a method for determining an efficient threshold for the binary classification. In this paper, a nonlinear optimization model is formulated based on minimizing the distance between MTS Receiver Operating Characteristics (ROC) curve and the theoretical optimal point named Modified Mahalanobis Taguchi System (MMTS). To validate the MMTS classification efficacy, it has been benchmarked with Support Vector Machines (SVMs), Naive Bayes (NB), Probabilistic Mahalanobis Taguchi Systems (PTM), Synthetic Minority Oversampling Technique (SMOTE), Adaptive Conformal Transformation (ACT), Kernel Boundary Alignment (KBA), Hidden Naive Bayes (HNB), and other improved Naive Bayes algorithms. MMTS outperforms the benchmarked algorithms especially when the imbalance ratio is greater than 400. A real life case study on manufacturing sector is used to demonstrate the applicability of the proposed model and to compare its performance with Mahalanobis Genetic Algorithm (MGA). PMID:28811820

Characterizing Adsorption Performance of Granular Activated Carbon with Permittivity.

PubMed

Yang, Yang; Shi, Chao; Zhang, Yi; Ye, Jinghua; Zhu, Huacheng; Huang, Kama

2017-03-07

A number of studies have achieved the consensus that microwave thermal technology can regenerate the granular activated carbon (GAC) more efficiently and energy-conservatively than other technologies. In particular, in the microwave heating industry, permittivity is a crucial parameter. This paper developed two equivalent models to establish the relationship between effective complex permittivity and pore volume of the GAC. It is generally based on Maxwell-Garnett approximation (MGA) theory. With two different assumptions in the model, two quantificational expressions were derived, respectively. Permittivity measurements and Brunauer-Emmett-Teller (BET) testing had been introduced in the experiments. Results confirmed the two expressions, which were extremely similar. Theoretical and experimental graphs were matched. This paper set up a bridge which links effective complex permittivity and pore volume of the GAC. Furthermore, it provides a potential and convenient method for the rapid assisted characterization of the GAC in its adsorption performance.

Characterizing Adsorption Performance of Granular Activated Carbon with Permittivity

PubMed Central

Yang, Yang; Shi, Chao; Zhang, Yi; Ye, Jinghua; Zhu, Huacheng; Huang, Kama

2017-01-01

A number of studies have achieved the consensus that microwave thermal technology can regenerate the granular activated carbon (GAC) more efficiently and energy-conservatively than other technologies. In particular, in the microwave heating industry, permittivity is a crucial parameter. This paper developed two equivalent models to establish the relationship between effective complex permittivity and pore volume of the GAC. It is generally based on Maxwell-Garnett approximation (MGA) theory. With two different assumptions in the model, two quantificational expressions were derived, respectively. Permittivity measurements and Brunauer–Emmett–Teller (BET) testing had been introduced in the experiments. Results confirmed the two expressions, which were extremely similar. Theoretical and experimental graphs were matched. This paper set up a bridge which links effective complex permittivity and pore volume of the GAC. Furthermore, it provides a potential and convenient method for the rapid assisted characterization of the GAC in its adsorption performance. PMID:28772628

A Grobner Basis Solution for Lightning Ground Flash Fraction Retrieval

NASA Technical Reports Server (NTRS)

Solakiewicz, Richard; Attele, Rohan; Koshak, William

2011-01-01

A Bayesian inversion method was previously introduced for retrieving the fraction of ground flashes in a set of flashes observed from a (low earth orbiting or geostationary) satellite lightning imager. The method employed a constrained mixed exponential distribution model to describe the lightning optical measurements. To obtain the optimum model parameters, a scalar function was minimized by a numerical method. In order to improve this optimization, we introduce a Grobner basis solution to obtain analytic representations of the model parameters that serve as a refined initialization scheme to the numerical optimization. Using the Grobner basis, we show that there are exactly 2 solutions involving the first 3 moments of the (exponentially distributed) data. When the mean of the ground flash optical characteristic (e.g., such as the Maximum Group Area, MGA) is larger than that for cloud flashes, then a unique solution can be obtained.

A Simple Network to Remove Interference in Surface EMG Signal from Single Gene Affected Phenylketonuria Patients for Proper Diagnosis

NASA Astrophysics Data System (ADS)

Mohanty, Madhusmita; Basu, Mousumi; Pattanayak, Deba Narayan; Mohapatra, Sumant Kumar

2018-04-01

Recently Autosomal Recessive Single Gene (ARSG) diseases are highly effective to the children within the age of 5-10 years. One of the most ARSG disease is a Phenylketonuria (PKU). This single gene disease is associated with mutations in the gene that encodes the enzyme phenylalanine hydroxylase (PAH, Gene 612349). Through this mutation process, PAH of the gene affected patient can not properly manufacture PAH as a result the patients suffer from decreased muscle tone which shows abnormality in EMG signal. Here the extraction of the quality of the PKU affected EMG (PKU-EMG) signal is a keen interest, so it is highly necessary to remove the added ECG signal as well as the biological and instrumental noises. In the Present paper we proposed a method for detection and classification of the PKU affected EMG signal. Here Discrete Wavelet Transformation is implemented for extraction of the features of the PKU affected EMG signal. Adaptive Neuro-Fuzzy Inference System (ANFIS) network is used for the classification of the signal. Modified Particle Swarm Optimization (MPSO) and Modified Genetic Algorithm (MGA) are used to train the ANFIS network. Simulation result shows that the proposed method gives better performance as compared to existing approaches. Also it gives better accuracy of 98.02% for the detection of PKU-EMG signal. The advantages of the proposed model is to use MGA and MPSO to train the parameters of ANFIS network for classification of ECG and EMG signal of PKU affected patients. The proposed method obtained the high SNR (18.13 ± 0.36 dB), SNR (0.52 ± 1.62 dB), RE (0.02 ± 0.32), MSE (0.64 ± 2.01), CC (0.99 ± 0.02), RMSE (0.75 ± 0.35) and MFRE (0.01 ± 0.02), RMSE (0.75 ± 0.35) and MFRE (0.01 ± 0.02). From authors knowledge, this is the first time a composite method is used for diagnosis of PKU affected patients. The accuracy (98.02%), sensitivity (100%) and specificity (98.59%) helps for proper clinical treatment. It can help for readers/researchers to improve the aforesaid performance for future prospective.

Extraction of Late Summer Sea Ice Properties from Polarimetric SAR Features in C- and X-Band

NASA Astrophysics Data System (ADS)

Fors, Ane S.; Brekke, Camilla; Gerland, Sebastian; Doulgeris, Anthony P.; Eltoft, Torbjørn

2015-04-01

In this study we examine the potential use of six polarimetric features for interpretation of late summer sea ice types. Five high-resolution C and X-band scenes were recorded in the Fram Strait covering fast first-year and old sea ice. In addition sea ice thickness, surface roughness and melt pond fraction were collected during a helicopter flight at the study area. From the SAR scenes, six polarimetric features were extracted. Along sections of the track of the helicopter flight, the mean of the SAR features were compared to mean values of the properties measured during the helicopter flight. The results reveal relations between several of the SAR features and the geophysical properties measured in C-band, and weak relations in X-band.

Collection of LAI and FPAR Data Over The Terra Core Sites

NASA Technical Reports Server (NTRS)

Myneni, Ranga B.; Knjazihhin, J.; Tian, Y.; Wang, Y.

2001-01-01

The objective of our effort was to collect and archive data on LAI (leaf area index) and FPAR (Fraction of Photosynthetically active Radiation absorbed by vegetation) at the EOS Core validation sites as well as to validate and evaluate global fields of LAI and FPAR derived from atmospherically corrected MODIS (Moderate Resolution Imaging Spectrometer) surface reflectance data by comparing these fields with the EOS Core validation data set. The above has been accomplished by: (a) the participation in selected field campaigns within the EOS Validation Program; (b) the processing of the collected data so that suitable comparison between field measurements and the MODIS LAI/FPAR fields can be made; (c) the comparison of the MODAS LAI/FRAM fields with the EOS Terra Core validation data set.

Fabrication and characterization of lead-free BaTiO3 thin film for storage device applications

NASA Astrophysics Data System (ADS)

Sharma, Hakikat; Negi, N. S.

2018-05-01

The lead-free BaTiO3 (BT) thin film solution has been prepared by sol-gel method. The prepared solution spin coated on Pt/TiO2/SiO2/ Si substrate. The fabricated thin film was analyzed by XRD and Raman spectrometer for structural conformation. Uniformity of thin film was examined by Atomic force microscope (AFM). Thickness of the film was measured by cross sectional FESEM. Activation energies for both positive and negative biasing have been calculated from temperature dependent leakage current density as a function of electric field. For ferroelectric memory devices such as FRAM the hysteresis loop plays important role. Electric filed dependent polarization of BT thin film measured at different switching voltages. With increasing voltage maximum polarization increases.

Active/passive microwave sensor comparison of MIZ-ice concentration estimates. [Marginal Ice Zone (MIZ)

NASA Technical Reports Server (NTRS)

Burns, B. A.; Cavalieri, D. J.; Keller, M. R.

1986-01-01

Active and passive microwave data collected during the 1984 summer Marginal Ice Zone Experiment in the Fram Strait (MIZEX 84) are used to compare ice concentration estimates derived from synthetic aperture radar (SAR) data to those obtained from passive microwave imagery at several frequencies. The comparison is carried out to evaluate SAR performance against the more established passive microwave technique, and to investigate discrepancies in terms of how ice surface conditions, imaging geometry, and choice of algorithm parameters affect each sensor. Active and passive estimates of ice concentration agree on average to within 12%. Estimates from the multichannel passive microwave data show best agreement with the SAR estimates because the multichannel algorithm effectively accounts for the range in ice floe brightness temperatures observed in the MIZ.

Observation of variations in the composition of sea ice in the Greenland MIZ during early summer 1983 with the Nimbus-7 SMMR. [Marginal Ice Zone (MIZ); Scanning Multichannel Microwave radiometer (SMMR)

NASA Technical Reports Server (NTRS)

Gloersen, P.; Campbell, W. J.

1984-01-01

Data acquired with the Scanning Multichannel Microwave Radiometer (SMMR) on board the Nimbus-7 Satellite for a six-week period in Fram Strait were analyzed with a procedure for calculating sea ice concentration, multiyear fraction, and ice temperature. Calculations were compared with independent observations made on the surface and from aircraft to check the validity of the calculations based on SMMR data. The calculation of multiyear fraction, which was known to be invalid near the melting point of sea ice, is discussed. The indication of multiyear ice is found to disappear a number of times, presumably corresponding to freeze/thaw cycles which occurred in this time period.

Variations in the structural and functional diversity of zooplankton over vertical and horizontal environmental gradients en route to the Arctic Ocean through the Fram Strait.

PubMed

Gluchowska, Marta; Trudnowska, Emilia; Goszczko, Ilona; Kubiszyn, Anna Maria; Blachowiak-Samolyk, Katarzyna; Walczowski, Waldemar; Kwasniewski, Slawomir

2017-01-01

A multi-scale approach was used to evaluate which spatial gradient of environmental variability is the most important in structuring zooplankton diversity in the West Spitsbergen Current (WSC). The WSC is the main conveyor of warm and biologically rich Atlantic water to the Arctic Ocean through the Fram Strait. The data set included 85 stratified vertical zooplankton samples (obtained from depths up to 1000 metres) covering two latitudinal sections (76°30'N and 79°N) located across the multi-path WSC system. The results indicate that the most important environmental variables shaping the zooplankton structural and functional diversity and standing stock variability are those associated with depth, whereas variables acting in the horizontal dimension are of lesser importance. Multivariate analysis of the zooplankton assemblages, together with different univariate descriptors of zooplankton diversity, clearly illustrated the segregation of zooplankton taxa in the vertical plane. The epipelagic zone (upper 200 m) hosted plentiful, Oithona similis-dominated assemblages with a high proportion of filter-feeding zooplankton. Although total zooplankton abundance declined in the mesopelagic zone (200-1000 m), zooplankton assemblages in that zone were more diverse and more evenly distributed, with high contributions from both herbivorous and carnivorous taxa. The vertical distribution of integrated biomass (mg DW m-2) indicated that the total zooplankton biomass in the epipelagic and mesopelagic zones was comparable. Environmental gradients acting in the horizontal plane, such as the ones associated with different ice cover and timing of the spring bloom, were reflected in the latitudinal variability in protist community structure and probably caused differences in succession in the zooplankton community. High abundances of Calanus finmarchicus in the WSC core branch suggest the existence of mechanisms advantageous for higher productivity or/and responsible for physical concentration of zooplankton. Our results indicate that regional hydrography plays a primary role in shaping zooplankton variability in the WSC on the way to the Arctic Ocean, with additional effects caused by biological factors related to seasonality in pelagic ecosystem development, resulting in regional differences in food availability or biological production between the continental slope and the deep ocean regions.

Exploring Arctic history through scientific drilling

NASA Astrophysics Data System (ADS)

ODP Leg 151 Shipboard Scientific Party

During the brief Arctic summer of 1993, the Ocean Drilling Program's research vessel JOIDES Resolution recovered the first scientific drill cores from the eastern Arctic Ocean. Dodging rafts of pack ice shed from the Arctic ice cap, the science party sampled sediments north of 80°N latitude from the Yermak Plateau, as well as from sites in Fram Strait, the northeastern Greenland margin, and the Iceland Plateau (Figure 1).The sediments collected reveal the earliest history of the connection between the North Atlantic and Arctic Oceans through the Nordic Seas. The region between Greenland and Norway first formed a series of isolated basins, sometimes with restricted deep circulation, that eventually joined and allowed deep and surface Arctic Ocean water to invade the region. A record was also retrieved that shows major glaciation in the region began about 2.5 m.y.a.

US-ROK Action Sheet 34: Safeguards Application of a Hand-held Mechanically Cooled Germanium Spectrometer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dreyer, J.; Burks, M.; Ham, Y.

2015-10-20

This report summarizes results of Action Sheet 34 - for the cooperative efforts on the field testing and evaluation of a high-resolution, hand-held, gamma-ray spectrometer, known as SPG (Spectroscopic Planar Germanium), for safeguards application such as short notice inspections, UF6 analysis, enrichment determination, and other potential applications. The Spectroscopic Planar Germanium (SPG) has been demonstrated IAEA Physical Inventory Verification (PIV) in South Korea. This field test was a success and the feedback provided by KINAC, IAEA, and national laboratory staff was used to direct efforts to improve the instrument this year. Key points in this report include measurement results frommore » PIV, analysis of spectra with commercially available Ortec U235 and PC-FRAM, and completion of tripod and tungsten collimator and integration of user feedback.« less

Enhanced effects by 4-phenylbutyrate in combination with RTK inhibitors on proliferation in brain tumor cell models

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marino, Ana-Maria; Center for Molecular Medicine CMM, Karolinska University Hospital, Stockholm; Sofiadis, Anastasios

2011-07-22

Highlights: {yields} The histone deacetylase inhibitor 4-phenylbutyrate substantially enhance efficacy of the receptor tyrosine kinase inhibitors gefitinib or vandetanib in glioma and medulloblastoma cell lines. {yields} Cell death increases and clonogenic survival is reduced in the combination treatments, over mono-therapy. {yields} Combination treatments with these drugs may improve clinical outcome for cancer therapy. -- Abstract: We have investigated in vitro effects of anticancer therapy with the histone deacetylase inhibitor (HDACi) 4-phenylbutyrate (4-PB) combined with receptor tyrosine kinase inhibitors (RTKi) gefitinib or vandetanib on the survival of glioblastoma (U343MGa) and medulloblastoma (D324Med) cells. In comparison with individual effects of these drugs,more » combined treatment with gefitinib/4-PB or vandetanib/4-PB resulted in enhanced cell killing and reduced clonogenic survival in both cell lines. Our results suggest that combined treatment using HDACi and RTKi may beneficially affect the outcome of cancer therapy.« less

A simple rule of thumb for elegant prehension.

PubMed

Mon-Williams, M; Tresilian, J R

2001-07-10

Reaching out to grasp an object (prehension) is a deceptively elegant and skilled behavior. The movement prior to object contact can be described as having two components, the movement of the hand to an appropriate location for gripping the object, the "transport" component, and the opening and closing of the aperture between the fingers as they prepare to grip the target, the "grasp" component. The grasp component is sensitive to the size of the object, so that a larger grasp aperture is formed for wider objects; the maximum grasp aperture (MGA) is a little wider than the width of the target object and occurs later in the movement for larger objects. We present a simple model that can account for the temporal relationship between the transport and grasp components. We report the results of an experiment providing empirical support for our "rule of thumb." The model provides a simple, but plausible, account of a neural control strategy that has been the center of debate over the last two decades.

Multiobjective Genetic Algorithm applied to dengue control.

PubMed

Florentino, Helenice O; Cantane, Daniela R; Santos, Fernando L P; Bannwart, Bettina F

2014-12-01

Dengue fever is an infectious disease caused by a virus of the Flaviridae family and transmitted to the person by a mosquito of the genus Aedes aegypti. This disease has been a global public health problem because a single mosquito can infect up to 300 people and between 50 and 100 million people are infected annually on all continents. Thus, dengue fever is currently a subject of research, whether in the search for vaccines and treatments for the disease or efficient and economical forms of mosquito control. The current study aims to study techniques of multiobjective optimization to assist in solving problems involving the control of the mosquito that transmits dengue fever. The population dynamics of the mosquito is studied in order to understand the epidemic phenomenon and suggest strategies of multiobjective programming for mosquito control. A Multiobjective Genetic Algorithm (MGA_DENGUE) is proposed to solve the optimization model treated here and we discuss the computational results obtained from the application of this technique. Copyright © 2014 Elsevier Inc. All rights reserved.

Single-walled carbon nanotubes based chemiresistive genosensor for label-free detection of human rheumatic heart disease

NASA Astrophysics Data System (ADS)

Singh, Swati; Kumar, Ashok; Khare, Shashi; Mulchandani, Ashok; Rajesh

2014-11-01

A specific and ultrasensitive, label free single-walled carbon nanotubes (SWNTs) based chemiresistive genosensor was fabricated for the early detection of Streptococcus pyogenes infection in human causing rheumatic heart disease. The mga gene of S. pyogenes specific 24 mer ssDNA probe was covalently immobilized on SWNT through a molecular bilinker, 1-pyrenemethylamine, using carbodiimide coupling reaction. The sensor was characterized by the current-voltage (I-V) characteristic curve and scanning electron microscopy. The sensing performance of the sensor was studied with respect to changes in conductance in SWNT channel based on hybridization of the target S. pyogenes single stranded genomic DNA (ssG-DNA) to its complementary 24 mer ssDNA probe. The sensor shows negligible response to non-complementary Staphylococcus aureus ssG-DNA, confirming the specificity of the sensor only with S. pyogenes. The genosensor exhibited a linear response to S. pyogenes G-DNA from 1 to1000 ng ml-1 with a limit of detection of 0.16 ng ml-1.

Findings from the 2012 EBRI/MGA Consumer Engagement in Health Care Survey.

PubMed

Fronstin, Paul

2012-12-01

The 2012 EBRI/MGA Consumer Engagement in Health Care Survey finds continued slow growth in consumer-driven health plans: 10 percent of the population was enrolled in a CDHP, up from 7 percent in 2011. Enrollment in HDHPs remained at 16 percent. Overall, 18.6 million adults ages 21-64 with private insurance, representing 15.4 percent of that market, were either in a CDHP or were in an HDHP that was eligible for an HSA. When their children were counted, about 25 million individuals with private insurance, representing about 14.6 percent of the market, were either in a CDHP or an HSA-eligible plan. This study finds evidence that adults in a CDHP and those in an HDHP were more likely than those in a traditional plan to exhibit a number of cost-conscious behaviors. While CDHP enrollees, HDHP enrollees, and traditional-plan enrollees were about equally likely to report that they made use of quality information provided by their health plan, CDHP enrollees were more likely to use cost information and to try to find information about their doctors' costs and quality from sources other than the health plan. CDHP enrollees were more likely than traditional-plan enrollees to take advantage of various wellness programs, such as health-risk assessments, health-promotion programs, and biometric screenings. In addition, financial incentives mattered more to CDHP enrollees than to traditional-plan enrollees. It is clear that the underlying characteristics of the populations enrolled in these plans are different: Adults in a CDHP were significantly more likely to report being in excellent or very good health. Adults in a CDHP and those in a HDHP were significantly less likely to smoke than were adults in a traditional plan, and they were significantly more likely to exercise. CDHP and HDHP enrollees were also more likely than traditional-plan enrollees to be highly educated. As the CDHP and HDHP markets continue to expand and more enrollees are enrolled for longer periods of time, the sustained impact that these plans are having on cost, quality, and access to health care services can be better understood. The eight years of consumer engagement surveys reported here provide unique data from which to measure future changes in this evolving type of health insurance. A significant portion of the population reported using a smartphone or a tablet. Among them, as many as one-third reported using an application (app) for health-related purposes. Among those not using an app, about one-half were very interested in using one.

Sea ice studies in the Spitsbergen-Greenland area

NASA Technical Reports Server (NTRS)

Vinje, T. E. (Principal Investigator)

1976-01-01

The author has identified the following significant results. Detailed information on the outflow through the Fram Strait of ice from the Polar Ocean over shorter periods was obtained. It is found that the speed of the outflow may vary about 100% over periods of a few days. The core of the East Greenland Current is found between 2 deg E and 4 deg W. The speed of the surface water at 81 deg N is for a calm period estimated to be about 10 cm/s. A new surging glacier was discovered and new fronts of several glaciers were determined. The variation of the snow line with respect to distance from the coast was for the first time determined for the southern part of Spitsbergen. Great variations were observed, from 200 m in east to 550 m in the central area of the island.

Pteropods counter mechanical damage and dissolution through extensive shell repair.

PubMed

Peck, Victoria L; Oakes, Rosie L; Harper, Elizabeth M; Manno, Clara; Tarling, Geraint A

2018-01-17

The dissolution of the delicate shells of sea butterflies, or pteropods, has epitomised discussions regarding ecosystem vulnerability to ocean acidification over the last decade. However, a recent demonstration that the organic coating of the shell, the periostracum, is effective in inhibiting dissolution suggests that pteropod shells may not be as susceptible to ocean acidification as previously thought. Here we use micro-CT technology to show how, despite losing the entire thickness of the original shell in localised areas, specimens of polar species Limacina helicina maintain shell integrity by thickening the inner shell wall. One specimen collected within Fram Strait with a history of mechanical and dissolution damage generated four times the thickness of the original shell in repair material. The ability of pteropods to repair and maintain their shells, despite progressive loss, demonstrates a further resilience of these organisms to ocean acidification but at a likely metabolic cost.

An FPGA-Based Test-Bed for Reliability and Endurance Characterization of Non-Volatile Memory

NASA Technical Reports Server (NTRS)

Rao, Vikram; Patel, Jagdish; Patel, Janak; Namkung, Jeffrey

2001-01-01

Memory technologies are divided into two categories. The first category, nonvolatile memories, are traditionally used in read-only or read-mostly applications because of limited write endurance and slow write speed. These memories are derivatives of read only memory (ROM) technology, which includes erasable programmable ROM (EPROM), electrically-erasable programmable ROM (EEPROM), Flash, and more recent ferroelectric non-volatile memory technology. Nonvolatile memories are able to retain data in the absence of power. The second category, volatile memories, are random access memory (RAM) devices including SRAM and DRAM. Writing to these memories is fast and write endurance is unlimited, so they are most often used to store data that change frequently, but they cannot store data in the absence of power. Nonvolatile memory technologies with better future potential are FRAM, Chalcogenide, GMRAM, Tunneling MRAM, and Silicon-Oxide-Nitride-Oxide-Silicon (SONOS) EEPROM.

Impact of Greenland orography on the Atlantic Meridional Overturning Circulation

NASA Astrophysics Data System (ADS)

Davini, Paolo; von Hardenberg, Jost; Filippi, Luca; Provenzale, Antonello

2015-04-01

We show that the absence of the Greenland Ice Sheet would have important consequences on the North Atlantic Ocean circulation, even without taking into account the effect of the freshwater input from ice melting. These effects are investigated in a 200-year long coupled ocean-atmosphere simulation with the high-resolution global climate model EC-Earth 3.0.1. Once a new equilibrium is established, cooling of Eurasia and of the North Atlantic and poleward shift of the subtropical jet are observed. These hemispheric changes are ascribed to a weakening of the Atlantic Meridional Overturning Circulation (AMOC) by about 20%. Such slowdown is associated to the freshening of the Arctic basin and to the related reduction in the freshwater export through the Fram Strait, as a result of the new wind pattern generated by the lower orography. This idealized experiment reveals the possibility of decreasing the AMOC by locally changing the surface winds.

Enhanced sea-ice export from the Arctic during the Younger Dryas.

PubMed

Not, Christelle; Hillaire-Marcel, Claude

2012-01-31

The Younger Dryas cold spell of the last deglaciation and related slowing of the Atlantic meridional overturning circulation have been linked to a large array of processes, notably an influx of fresh water into the North Atlantic related to partial drainage of glacial Lake Agassiz. Here we observe a major drainage event, in marine sediment cores raised from the Lomonosov Ridge, in the central Arctic Ocean marked by a pulse in detrital dolomitic-limestones. This points to an Arctic-Canadian sediment source area with about fivefold higher Younger Dryas ice-rafting deposition rate, in comparison with the Holocene. Our findings thus support the hypothesis of a glacial drainage event in the Canadian Arctic area, at the onset of the Younger Dryas, enhancing sea-ice production and drifting through the Arctic, then export through Fram Strait, towards Atlantic meridional overturning circulation sites of the northern North Atlantic.

Results and Implications of Mineralogical Models for Chemical Sediments at Meridiani Planum

NASA Technical Reports Server (NTRS)

Clark, B. C.; McLennan, S. M.; Morris, R. V.; Gellert, R.; Jolliff, B.; Knoll, A.; Lowenstein, T. K.; Ming, D. W.; Tosca, N. J.; Christensen, P. R.

2005-01-01

The Mars Exploration Rover (MER) "Opportunity" has explored chemically-enriched sedimentary outcrops at Meridiani Planum, Mars. In its first year, three different crater sites - Eagle, Fram and Endurance - have been explored. Nineteen high-interest outcrop rocks were investigated by first grinding a hole to reach the interior (using the Rock Abrasion Tool, RAT), and then conducting APXS (alpha particle x-ray spectrometry) analysis, MB (M ssbauer) analysis, and close up imaging (MI, microscopic imager). Sixteen elements and four Fe-bearing minerals were assayed to good accuracy in each sample, producing 380 compositional data points. The Miniature Thermal Emission Spectrometer (Mini- TES) obtained spectra on outcrop materials which provide direct indication of several mineral classes. Preliminary reports on Eagle crater and three RAT samples have been published. Chemical trends and a derived mineralogical model for all RAT d outcrop samples to date has been developed.

Ku band airborne radar altimeter observations of marginal sea ice during the 1984 Marginal Ice Zone Experiment

NASA Technical Reports Server (NTRS)

Drinkwater, Mark R.

1991-01-01

Pulse-limited, airborne radar data taken in June and July 1984 with a 13.8-GHz altimeter over the Fram Strait marginal ice zone are analyzed with the aid of large-format aerial photography, airborne synthetic aperture radar data, and surface observations. Variations in the radar return pulse waveforms are quantified and correlated with ice properties recorded during the Marginal Ice Zone Experiment. Results indicate that the wide-beam altimeter is a flexible instrument, capable of identifying the ice edge with a high degree of accuracy, calculating the ice concentration, and discriminating a number of different ice classes. This suggests that microwave radar altimeters have a sensitivity to sea ice which has not yet been fully exploited. When fused with SSM/I, AVHRR and ERS-1 synthetic aperture radar imagery, future ERS-1 altimeter data are expected to provide some missing pieces to the sea ice geophysics puzzle.

Multisensor comparison of ice concentration estimates in the marginal ice zone

NASA Technical Reports Server (NTRS)

Burns, B. A.; Cavalieri, D. J.; Gloersen, P.; Keller, M. R.; Campbell, W. J.

1987-01-01

Aircraft remote sensing data collected during the 1984 summer Marginal Ice Zone Experiment in the Fram Strait are used to compare ice concentration estimates derived from synthetic aperture radar (SAR) imagery, passive microwave imagery at several frequencies, aerial photography, and spectral photometer data. The comparison is carried out not only to evaluate SAR performance against more established techniques but also to investigate how ice surface conditions, imaging geometry, and choice of algorithm parameters affect estimates made by each sensor.Active and passive microwave sensor estimates of ice concentration derived using similar algorithms show an rms difference of 13 percent. Agreement between each microwave sensor and near-simultaneous aerial photography is approximately the same (14 percent). The availability of high-resolution microwave imagery makes it possible to ascribe the discrepancies in the concentration estimates to variations in ice surface signatures in the scene.

Variations of mesoscale and large-scale sea ice morphology in the 1984 Marginal Ice Zone Experiment as observed by microwave remote sensing

NASA Technical Reports Server (NTRS)

Campbell, W. J.; Josberger, E. G.; Gloersen, P.; Johannessen, O. M.; Guest, P. S.

1987-01-01

The data acquired during the summer 1984 Marginal Ice Zone Experiment in the Fram Strait-Greenland Sea marginal ice zone, using airborne active and passive microwave sensors and the Nimbus 7 SMMR, were analyzed to compile a sequential description of the mesoscale and large-scale ice morphology variations during the period of June 6 - July 16, 1984. Throughout the experiment, the long ice edge between northwest Svalbard and central Greenland meandered; eddies were repeatedly formed, moved, and disappeared but the ice edge remained within a 100-km-wide zone. The ice pack behind this alternately diffuse and compact edge underwent rapid and pronounced variations in ice concentration over a 200-km-wide zone. The high-resolution ice concentration distributions obtained in the aircraft images agree well with the low-resolution distributions of SMMR images.

Microwave and physical properties of sea ice in the winter marginal ice zone

NASA Technical Reports Server (NTRS)

Tucker, W. B., III; Perovich, D. K.; Gow, A. J.; Grenfell, T. C.; Onstott, R. G.

1991-01-01

Surface-based active and passive microwave measurements were made in conjunction with ice property measurements for several distinct ice types in the Fram Strait during March and April 1987. Synthesis aperture radar imagery downlinked from an aircraft was used to select study sites. The surface-based radar scattering cross section and emissivity spectra generally support previously inferred qualitative relationships between ice types, exhibiting expected separation between young, first-year and multiyear ice. Gradient ratios, calculated for both active and passive data, appear to allow clear separation of ice types when used jointly. Surface flooding of multiyear floes, resulting from excessive loading and perhaps wave action, causes both active and passive signatures to resemble those of first-year ice. This effect could possibly cause estimates of ice type percentages in the marginal ice zone to be in error when derived from aircraft- or satellite-born sensors.

A 26 million year gap in the central Arctic record at the greenhouse-icehouse transition: Looking for clues

USGS Publications Warehouse

Sangiorgi, F.; Brumsack, H.-J.; Willard, D.A.; Schouten, S.; Stickley, C.E.; O'Regan, M.; Reichart, G.-J.; Sinninghe, Damste J.S.; Brinkhuis, H.

2008-01-01

The Cenozoic record of the Lomonosov Ridge (central Arctic Ocean) recovered during Integrated Ocean Drilling Program (IODP) Expedition 302 revealed an unexpected 26 Ma hiatus, separating middle Eocene (???44.4 Ma) from lower Miocene sediments (???18.2 Ma). To elucidate the nature of this unconformity, we performed a multiproxy palynological (dinoflagellate cysts, pollen, and spores), micropaleontological (siliceous microfossils), inorganic, and organic (Tetra Ether Index of lipids with 86 carbon atoms (TEX86) and Branched and Isoprenoid Tetraether (BIT)) geochemical analysis of the sediments from ???5 m below to ???7 m above the hiatus. Four main paleoenvironmental. phases (A-D) are recognized in the sediments encompassing the unconformity, two below (A-B) and two above (C-D): (A) Below the hiatus, proxies show relatively warm temperatures, with Sea Surface Temperatures (TEX86-derived SSTs) of about 8??C and high fresh to brackish water influence. (B) Approaching the hiatus, proxies indicate a cooling trend (TEX86-derived SSTs of ???5??C), increased freshwater influence, and progressive shoaling of the Lomonosov Ridge drilling site, located close to or at sea level. (C) The interval directly above the unconformity contains sparse reworked Cretaceous to Oligocene dinoflagellate cysts. Sediments were deposited in a relatively shallow, restricted marine environment. Proxies show the simultaneous influence of both fresh and marine waters, with alternating oxic and anoxic conditions. Pollen indicates a relatively cold climate. Intriguingly, TEX86-derived SSTs are unexpectedly high, ???15-19??C. Such warm surface waters may be partially explained by the ingression of warmer North Atlantic waters after the opening of the Fram Strait during the early Miocene. (D) Sediments of the uppermost interval indicate a phase of extreme oxic conditions, and a well-ventilated environment, which occurred after the complete opening of the Fram Strait. Importantly, and in contrast with classical postrifting thermal subsidence models for passive margins, our data suggest that sediment erosion and/or nondeposition that generated the hiatus was likely due to a progressive shoaling of the Lomonosov Ridge. A shallow water setting both before and after the hiatus suggests that the Lomonosov Ridge remained at or near sea level for the duration of the gap in the sedimentary record. Interacting sea level changes and/ or tectonic activity (possibly uplift) must be invoked as possible causes for such a long hiatus. Copyright 2008 by the American Geophysical Union.

Carbon flows in the benthic food web at the deep-sea observatory HAUSGARTEN (Fram Strait)

NASA Astrophysics Data System (ADS)

van Oevelen, Dick; Bergmann, Melanie; Soetaert, Karline; Bauerfeind, Eduard; Hasemann, Christiane; Klages, Michael; Schewe, Ingo; Soltwedel, Thomas; Budaeva, Nataliya E.

2011-11-01

The HAUSGARTEN observatory is located in the eastern Fram Strait (Arctic Ocean) and used as long-term monitoring site to follow changes in the Arctic benthic ecosystem. Linear inverse modelling was applied to decipher carbon flows among the compartments of the benthic food web at the central HAUSGARTEN station (2500 m) based on an empirical data set consisting of data on biomass, prokaryote production, total carbon deposition and community respiration. The model resolved 99 carbon flows among 4 abiotic and 10 biotic compartments, ranging from prokaryotes up to megafauna. Total carbon input was 3.78±0.31 mmol C m -2 d -1, which is a comparatively small fraction of total primary production in the area. The community respiration of 3.26±0.20 mmol C m -2 d -1 is dominated by prokaryotes (93%) and has lower contributions from surface-deposit feeding macro- (1.7%) and suspension feeding megafauna (1.9%), whereas contributions from nematode and other macro- and megabenthic compartments were limited to <1%. The high prokaryotic contribution to carbon processing suggests that functioning of the benthic food web at the central HAUSGARTEN station is comparable to abyssal plain sediments that are characterised by strong energy limitation. Faunal diet compositions suggest that labile detritus is important for deposit-feeding nematodes (24% of their diet) and surface-deposit feeding macrofauna (˜44%), but that semi-labile detritus is more important in the diets of deposit-feeding macro- and megafauna. Dependency indices on these food sources were also calculated as these integrate direct (i.e. direct grazing and predator-prey interactions) and indirect (i.e. longer loops in the food web) pathways in the food web. Projected sea-ice retreats for the Arctic Ocean typically anticipate a decrease in the labile detritus flux to the already food-limited benthic food web. The dependency indices indicate that faunal compartments depend similarly on labile and semi-labile detritus, which suggests that the benthic biota may be more sensitive to changes in labile detritus inputs than when assessed from diet composition alone. Species-specific responses to different types of labile detritus inputs, e.g. pelagic algae versus sympagic algae, however, are presently unknown and are needed to assess the vulnerability of individual components of the benthic food web.

Space/Time Statistics of Polar Ice Motion

NASA Technical Reports Server (NTRS)

Emery, William J.; Fowler, Charles; Maslanik, James A.

2003-01-01

Ice motions have been computed from passive microwave imagery (SMMR and SSM/I) on a daily basis for both Polar Regions. In the Arctic these daily motions have been merged with daily motions from AVHRR imagery and the Arctic buoy program. In the Antarctic motion only from the AVHRR were available for merging with the passive microwave vectors. Long-term means, monthly means and weekly means have all been computed from the resulting 22-year time series of polar ice motion. Papers are in preparation that present the long term (22 year) means, their variability and show animations of the monthly means over this time period for both Polar Regions. These papers will have links to "enhanced objects" that allow the reader to view the animations as part of the paper. The first paper presents the ice motion results from each of the Polar Regions. The second paper looks only at ice motion in the Arctic in order to develop a time series of ice age in the Arctic. Starting with the first full SMMR year in 1979 we keep track of each individual "ice element" (resolution of the sensor) and track it in the subsequent monthly time series. After a year we "age" each "particle" and we thus can keep track of the age of the ice starting in 1979. We keep track of ice age classes between one and five years and thus we can see the evolution of the ice as it ages after the initial 5-year period. This calculation shows how we are losing the older ice through Fram Strait at a rather alarming rate particularly in the past 15 years. This loss of older ice has resulted in an overall decrease in the thickest, oldest ice, which is now limited to a region just north of the Canadian Archipelago with tongues extending out across the pole towards the Siberian Shelf. This loss of old ice is consistent with the effects of global warming which provides the heat needed to melt, move and disperse this oldest ice through Fram Strait. This is the first step in a progression that may eventually open the Arctic ice pack and lead to an ice-free Arctic Ocean.

Pliocene-Pleistocene changes in Arctic sea-ice cover: New biomarker records from Fram Strait/Yermak Plateau (ODP Sites 911 and 912)

NASA Astrophysics Data System (ADS)

Stein, Ruediger; Fahl, Kirsten

2013-04-01

Recently, a novel and promising biomarker proxy for reconstruction of Arctic sea-ice conditions was developed and is based on the determination of a highly branched isoprenoid with 25 carbons (IP25; Belt et al., 2007). Following this pioneer IP25 study by Belt and colleagues, several IP25 studies of marine surface sediments and sediment cores as well as sediment trap samples from northpolar areas were carried out successfully and allowed detailed reconstruction of modern and late Quaternary sea ice variability in these regions (e.g., Massé et al., 2008; Müller et al., 2009, 2011; Vare et al., 2009; Belt et al., 2010; Fahl and Stein, 2012; for review see Stein et al., 2012). Here, we present new (low-resolution) biomarker records from Ocean Drilling Program (ODP) Sites 911 and 912, representing the Pliocene-Pleistocene time interval (including the interval of major intensification of Northern Hemisphere Glaciation near 2.7 Ma). These data indicate that sea ice of variable extent was present in the Fram Strait/southern Yermak Plateau area during most of the time period under investigation. In general, an increase in sea-ice cover seems to correlate with phases of extended late Pliocene-Pleistocene continental ice-sheets. At ODP Site 912, a significant increase in sea-ice extension occurred near 1.2 Ma (Stein and Fahl, 2012). Furthermore, our data support the idea that a combination of IP25 and open water, phytoplankton biomarker data ("PIP25 index"; Müller et al., 2011) may give more reliable and quantitative estimates of past sea-ice cover (at least for the study area). This study reveals that the novel IP25/PIP25 biomarker approach has potential for semi-quantitative paleo-sea ice studies covering the entire Quaternary and motivate to carry out further detailed high-resolution research on ODP/IODP material using this proxy. References Belt, S.T., Massé, G., Rowland, S.J., Poulin, M., Michel, C., LeBlanc, B., 2007. A novel chemical fossil of palaeo sea ice: IP25. Organic Geochemistry 38, 16-27. Belt, S.T., Vare, L.L., Massé, G., Manners, H.R., Price, J.C., MacLachlan, S.E., Andrews, J.T., Schmidt, S., 2010. Striking similarities in temporal changes to spring sea ice occurrence across the central Canadian Arctic Archipelago over the last 7000 years. Quaternary Science Reviews 29, 3489-3504. Fahl, K. and Stein, R., 2012. Modern seasonal variability and deglacial/Holocene change of central Arctic Ocean sea-ice cover: New insights from biomarker proxy records. Earth Planetary Science Letters 351-352C, 123-133, doi:10.1016/j.epsl.2012.07.009. Massé, G., Rowland, S.J., Sicre, M.-A., Jacob, J., Jansen, E., Belt, S.T., 2008. Abrupt climate changes for Iceland during the last millennium: Evidence from high resolution sea ice reconstructions. Earth Planetary Science Letters 269, 565-569. Müller, J., Massé, G., Stein, R., Belt, S.T., 2009. Variability of sea-ice conditions in the Fram Strait over the past 30,000 years. Nature Geoscience 2, 772-776. Müller, J., Wagner, A., Fahl, K., Stein, R., Prange, M., Lohmann, G., 2011. Towards quantitative sea ice reconstructions in the northern North Atlantic: A combined biomarker and numerical modelling approach. Earth Planetary Science Letters 306, 137-148. Stein, R. and Fahl, K., 2012. Biomarker proxy IP25 shows potential for studying entire Quaternary Arctic sea-ice history. Organic Geochemistry; doi: 10.1016/j.orggeochem.2012.11.005. Stein, R., Fahl, K., and Müller, J., 2012. Proxy reconstruction of Arctic Ocean sea ice history: "From IRD to IP25". Polarforschung 82, 37-71. Vare, L.L., Massé, G., Gregory, T.R., Smart, C.W., Belt, S.T., 2009. Sea ice variations in the central Canadian Arctic Archipelago during the Holocene. Quaternary Science Reviews 28, 1354-1366.

[Scientific Evaluation of Crude Drugs and Kampo Medicines Using the Eastern Blotting Method and Its Application to Biological Metabolic Studies].

PubMed

Morinaga, Osamu

2018-01-01

　The scientific evaluation of crude drugs and kampo medicines (KMs) was demonstrated using the eastern blotting method with monoclonal antibodies (MAbs) against bioactive natural compounds. Scutellariae radix is one of the most important crude drugs used in KMs. Part of its pharmaceutical properties is due to the flavone glycoside baicalin (BI). A quantitative analysis method based on eastern blotting was developed for BI using an anti-BI MAb. A rapid, simple, sensitive, specific analytical system was subsequently established for BI with the eastern blotting technique using dot-blot and chemiluminescent methods. This system was useful as a high-throughput analytical method for the determination of BI in KMs as well as HPLC and enzyme-linked immunosorbent assay systems. Furthermore, an eastern blotting method was applied to the biological metabolic study of glycyrrhizic acid (GL), the major active constituent of licorice, for investigation of metabolites of GL such as 3-monoglucuronyl-glycyrrhetinic acid (3MGA) because licorice causes pseudoaldosteronism as a side effect. This approach may make it possible to determine the pathogenic agents of licorice-induced pseudoaldosteronism.

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas.

PubMed

Schaub, Franz X; Dhankani, Varsha; Berger, Ashton C; Trivedi, Mihir; Richardson, Anne B; Shaw, Reid; Zhao, Wei; Zhang, Xiaoyang; Ventura, Andrea; Liu, Yuexin; Ayer, Donald E; Hurlin, Peter J; Cherniack, Andrew D; Eisenman, Robert N; Bernard, Brady; Grandori, Carla

2018-03-28

Although the MYC oncogene has been implicated in cancer, a systematic assessment of alterations of MYC, related transcription factors, and co-regulatory proteins, forming the proximal MYC network (PMN), across human cancers is lacking. Using computational approaches, we define genomic and proteomic features associated with MYC and the PMN across the 33 cancers of The Cancer Genome Atlas. Pan-cancer, 28% of all samples had at least one of the MYC paralogs amplified. In contrast, the MYC antagonists MGA and MNT were the most frequently mutated or deleted members, proposing a role as tumor suppressors. MYC alterations were mutually exclusive with PIK3CA, PTEN, APC, or BRAF alterations, suggesting that MYC is a distinct oncogenic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such as immune response and growth factor signaling; chromatin, translation, and DNA replication/repair were conserved pan-cancer. This analysis reveals insights into MYC biology and is a reference for biomarkers and therapeutics for cancers with alterations of MYC or the PMN. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

RAG-mediated recombination is the predominant driver of oncogenic rearrangement in ETV6-RUNX1 acute lymphoblastic leukemia

PubMed Central

Papaemmanuil, Elli; Rapado, Inmaculada; Li, Yilong; Potter, Nicola E; Wedge, David C; Tubio, Jose; Alexandrov, Ludmil B; Van Loo, Peter; Cooke, Susanna L; Marshall, John; Martincorena, Inigo; Hinton, Jonathan; Gundem, Gunes; van Delft, Frederik W; Nik-Zainal, Serena; Jones, David R; Ramakrishna, Manasa; Titley, Ian; Stebbings, Lucy; Leroy, Catherine; Menzies, Andrew; Gamble, John; Robinson, Ben; Mudie, Laura; Raine, Keiran; O’Meara, Sarah; Teague, Jon W; Butler, Adam P; Cazzaniga, Giovanni; Biondi, Andrea; Zuna, Jan; Kempski, Helena; Muschen, Markus; Ford, Anthony M; Stratton, Michael R; Greaves, Mel; Campbell, Peter J

2014-01-01

The ETV6-RUNX1 fusion gene, found in 25% of childhood acute lymphoblastic leukemia (ALL), is acquired in utero but requires additional somatic mutations for overt leukemia. We used exome and low-coverage whole-genome sequencing to characterize secondary events associated with leukemic transformation. RAG-mediated deletions emerge as the dominant mutational process, characterized by recombination signal sequence motifs near the breakpoints; incorporation of non-templated sequence at the junction; ~30-fold enrichment at promoters and enhancers of genes actively transcribed in B-cell development and an unexpectedly high ratio of recurrent to non-recurrent structural variants. Single cell tracking shows that this mechanism is active throughout leukemic evolution with evidence of localized clustering and re-iterated deletions. Integration of point mutation and rearrangement data identifies ATF7IP and MGA as two new tumor suppressor genes in ALL. Thus, a remarkably parsimonious mutational process transforms ETV6-RUNX1 lymphoblasts, targeting the promoters, enhancers and first exons of genes that normally regulate B-cell differentiation. PMID:24413735

Variational Identification of Markovian Transition States

NASA Astrophysics Data System (ADS)

Martini, Linda; Kells, Adam; Covino, Roberto; Hummer, Gerhard; Buchete, Nicolae-Viorel; Rosta, Edina

2017-07-01

We present a method that enables the identification and analysis of conformational Markovian transition states from atomistic or coarse-grained molecular dynamics (MD) trajectories. Our algorithm is presented by using both analytical models and examples from MD simulations of the benchmark system helix-forming peptide Ala5 , and of larger, biomedically important systems: the 15-lipoxygenase-2 enzyme (15-LOX-2), the epidermal growth factor receptor (EGFR) protein, and the Mga2 fungal transcription factor. The analysis of 15-LOX-2 uses data generated exclusively from biased umbrella sampling simulations carried out at the hybrid ab initio density functional theory (DFT) quantum mechanics/molecular mechanics (QM/MM) level of theory. In all cases, our method automatically identifies the corresponding transition states and metastable conformations in a variationally optimal way, with the input of a set of relevant coordinates, by accurately reproducing the intrinsic slowest relaxation rate of each system. Our approach offers a general yet easy-to-implement analysis method that provides unique insight into the molecular mechanism and the rare but crucial (i.e., rate-limiting) transition states occurring along conformational transition paths in complex dynamical systems such as molecular trajectories.

Single-walled carbon nanotubes based chemiresistive genosensor for label-free detection of human rheumatic heart disease

DOE Office of Scientific and Technical Information (OSTI.GOV)

Singh, Swati; Kumar, Ashok, E-mail: rajesh-csir@yahoo.com, E-mail: ashokigib@rediffmail.com; Academy of Scientific and Innovative Research

A specific and ultrasensitive, label free single-walled carbon nanotubes (SWNTs) based chemiresistive genosensor was fabricated for the early detection of Streptococcus pyogenes infection in human causing rheumatic heart disease. The mga gene of S. pyogenes specific 24 mer ssDNA probe was covalently immobilized on SWNT through a molecular bilinker, 1-pyrenemethylamine, using carbodiimide coupling reaction. The sensor was characterized by the current-voltage (I-V) characteristic curve and scanning electron microscopy. The sensing performance of the sensor was studied with respect to changes in conductance in SWNT channel based on hybridization of the target S. pyogenes single stranded genomic DNA (ssG-DNA) to itsmore » complementary 24 mer ssDNA probe. The sensor shows negligible response to non-complementary Staphylococcus aureus ssG-DNA, confirming the specificity of the sensor only with S. pyogenes. The genosensor exhibited a linear response to S. pyogenes G-DNA from 1 to1000 ng ml{sup −1} with a limit of detection of 0.16 ng ml{sup −1}.« less

Activated platelet-derived growth factor autocrine pathway drives the transformed phenotype of a human glioblastoma cell line.

PubMed

Vassbotn, F S; Ostman, A; Langeland, N; Holmsen, H; Westermark, B; Heldin, C H; Nistér, M

1994-02-01

Human glioblastoma cells (A172) were found to concomitantly express PDGF-BB and PDGF beta-receptors. The receptors were constitutively autophosphorylated in the absence of exogenous ligand, suggesting the presence of an autocrine PDGF pathway. Neutralizing PDGF antibodies as well as suramin inhibited the autonomous PDGF receptor tyrosine kinase activity and resulted in up-regulation of receptor protein. The interruption of the autocrine loop by the PDGF antibodies reversed the transformed phenotype of the glioblastoma cell, as determined by (1) diminished DNA synthesis, (2) inhibition of tumor colony growth, and (3) reversion of the transformed morphology of the tumor cells. The PDGF antibodies showed no effect on the DNA synthesis of another glioblastoma cells line (U-343MGa 31L) or on Ki-ras-transformed fibroblasts. The present study demonstrates an endogenously activated PDGF pathway in a spontaneous human glioblastoma cell line. Furthermore, we provide evidence that the autocrine PDGF pathway drives the transformed phenotype of the tumor cells, a process that can be blocked by extracellular antagonists.

Impact of Greenland orography on the Atlantic Meridional Overturning Circulation

NASA Astrophysics Data System (ADS)

Davini, P.; Hardenberg, J.; Filippi, L.; Provenzale, A.

2015-02-01

We show that the absence of the Greenland ice sheet would have important consequences on the North Atlantic Ocean circulation, even without taking into account the effect of the freshwater input to the ocean from ice melting. These effects are investigated in a 600year long coupled ocean-atmosphere simulation with the high-resolution global climate model EC-Earth 3.0.1. Once a new equilibrium is established, a cooling of Eurasia and of the North Atlantic and a poleward shift of the subtropical jet are observed. These hemispheric changes are ascribed to a weakening of the Atlantic Meridional Overturning Circulation (AMOC) by about 12%. We attribute this slowdown to a reduction in salinity of the Arctic basin and to the related change of the mass and salt transport through the Fram Strait—a consequence of the new surface wind pattern over the lower orography. This idealized experiment illustrates the sensitivity of the AMOC to local surface winds.

Data report on variations in the composition of sea ice during MIZEX/East'83 with the Nimbus-7 SMMR

NASA Technical Reports Server (NTRS)

Gloersen, P.

1984-01-01

Data acquired with the scanning multichannel microwave radiometer (SMMR) on board the Nimbus-7 satellite for a six-week period including the 1983 MIZEX in Fram Strait were analyzed with the use of a previously developed procedure for calculating sea ice concentration, multiyear fraction, and ice temperature. These calculations can compared with independent observations made on the surface and from aircraft in order to check the validity of the calculations based on SMMR data. The calculation of multiyear fraction, which was known earlier to be invalid near the melting point of sea ice, was of particular interest during this period. The indication of multiyear ice was found to disappear a number of times, presumably corresponding to freeze/thaw cycles which occurred in this time period. Both grid-print maps and grey-scale images of total sea ice concentration and multiyear sea ice fraction for the entire period are included.

Toward self-assembled ferroelectric random access memories: hard-wired switching capacitor arrays with almost Tb/in.(2) densities.

PubMed

Evans, Paul R; Zhu, Xinhau; Baxter, Paul; McMillen, Mark; McPhillips, John; Morrison, Finlay D; Scott, James F; Pollard, Robert J; Bowman, Robert M; Gregg, J Marty

2007-05-01

We report on the successful fabrication of arrays of switchable nanocapacitors made by harnessing the self-assembly of materials. The structures are composed of arrays of 20-40 nm diameter Pt nanowires, spaced 50-100 nm apart, electrodeposited through nanoporous alumina onto a thin film lower electrode on a silicon wafer. A thin film ferroelectric (both barium titanate (BTO) and lead zirconium titanate (PZT)) has been deposited on top of the nanowire array, followed by the deposition of thin film upper electrodes. The PZT nanocapacitors exhibit hysteresis loops with substantial remnant polarizations, while although the switching performance was inferior, the low-field characteristics of the BTO nanocapacitors show dielectric behavior comparable to conventional thin film heterostructures. While registration is not sufficient for commercial RAM production, this is nevertheless an embryonic form of the highest density hard-wired FRAM capacitor array reported to date and compares favorably with atomic force microscopy read-write densities.

Fluid-Evaporation Records Preserved in Meridiani Rocks

NASA Technical Reports Server (NTRS)

Rao, M. N.; Nyquist, Laurence E.; Sutton, S. R.

2009-01-01

We have shown earlier that the high SO3/Cl ratios found in secondary mineral assemblages in shergottite GRIM glasses (Gas-Rich Impact-Melt) likely resulted from interactions of regolith materials with sulfate-rich (and Cl-poor) solutions. The low SO3/Cl ratios determined in secondary salts in nakhalite fracture-fillings presumably formed by rock interactions with chloride-rich (and SO4-poor) solutions near Mars surface. The SO3 and Cl abundances determined by APXS in abraded rocks (RAT) from Endurance, Fram and Eagle craters indicate that these salt assemblages likely formed by evaporative concentration of brine fluids at Meridiani. The SO3/Cl ratios in the abraded rocks are examined here, instead of their absolute abundances, because the abundance ratios might provide better guide-lines for tracking the evolution of evaporating fluids at Meridiani. The SO3/Cl ratios in these samples, in turn, might provide clues for the mobile element ratios of the altering fluids that infiltrated into the Meridiani rocks.

MIZEX, 1984, NASA CV-990 flight report

NASA Technical Reports Server (NTRS)

1985-01-01

During June/July 1984, the NASA CV-990 Airborne Laboratory was utilized in a mission to overly the Fram Strait/East Greenland Sea marginal ice zone (MIZ) during the main summer marginal ice zone experiment (MIZEX '84). The eight data flights were coordinated where possible with overpasses of the Nimbus-7 satellite, and with measurement of sea ice, open ocean, and atmospheric properties at the surface. The surface research teams were based on seven research vessels, some with helicopters: (1) M/V Kvitbjorn, (2) M/V Polarqueen; (3) M/S Haakon Mosby; (4) a M/S H.U. Sverdrup, all from Norway; (5) F/S Polarstern from the Federal Republic of Germany; and (6) the USNS Lynch from the USA. There were also coordinated flights with the NRL P3, NOAA P3, Canadian CV580, and the French B-17 during the overlap portions of their respective missions. Analysis of the real-time data acquired during the mission and uncalibrated data stored on tape has served to indicate the mission was over 90% successful.

Satellite Test of Radiation Impact on Ramtron 512K FRAM

NASA Technical Reports Server (NTRS)

MacLeod, Todd C.; Sayyah, Rana; Sims, W. Herb; Varnavas, Kosta A.; Ho, Fat D.

2009-01-01

The Memory Test Experiment is a space test of a ferroelectric memory device on a low Earth orbit satellite. The test consists of writing and reading data with a ferroelectric based memory device. Any errors are detected and are stored on board the satellite. The data is send to the ground through telemetry once a day. Analysis of the data can determine the kind of error that was found and will lead to a better understanding of the effects of space radiation on memory systems. The test will be one of the first flight demonstrations of ferroelectric memory in a near polar orbit which allows testing in a varied radiation environment. The memory devices being tested is a Ramtron Inc. 512K memory device. This paper details the goals and purpose of this experiment as well as the development process. The process for analyzing the data to gain the maximum understanding of the performance of the ferroelectric memory device is detailed.

A 26 million year gap in the central Arctic record at the greenhouse-icehouse transition: Looking for clues

USGS Publications Warehouse

Sangiorgi, Francesca; Brumsack, Hans-Juergen; Willard, Debra A.; Schouten, Stefan; Stickley, Catherine E.; O'Regan, Matthew; Reichart, Gert-Jan; Damste, Jaap S. Sinninghe; Brinkhuis, Henk

2008-01-01

The Cenozoic record of the Lomonosov Ridge (central Arctic Ocean) recovered during Integrated Ocean Drilling Program (IODP) Expedition 302 revealed an unexpected 26 Ma hiatus, separating middle Eocene (∼44.4 Ma) from lower Miocene sediments (∼18.2 Ma). To elucidate the nature of this unconformity, we performed a multiproxy palynological (dinoflagellate cysts, pollen, and spores), micropaleontological (siliceous microfossils), inorganic, and organic (Tetra Ether Index of lipids with 86 carbon atoms (TEX86) and Branched and Isoprenoid Tetraether (BIT)) geochemical analysis of the sediments from ∼5 m below to ∼7 m above the hiatus. Four main paleoenvironmental phases (A–D) are recognized in the sediments encompassing the unconformity, two below (A–B) and two above (C–D): (A) Below the hiatus, proxies show relatively warm temperatures, with Sea Surface Temperatures (TEX86‐derived SSTs) of about 8°C and high fresh to brackish water influence. (B) Approaching the hiatus, proxies indicate a cooling trend (TEX86‐derived SSTs of ∼5°C), increased freshwater influence, and progressive shoaling of the Lomonosov Ridge drilling site, located close to or at sea level. (C) The interval directly above the unconformity contains sparse reworked Cretaceous to Oligocene dinoflagellate cysts. Sediments were deposited in a relatively shallow, restricted marine environment. Proxies show the simultaneous influence of both fresh and marine waters, with alternating oxic and anoxic conditions. Pollen indicates a relatively cold climate. Intriguingly, TEX86‐derived SSTs are unexpectedly high, ∼15–19°C. Such warm surface waters may be partially explained by the ingression of warmer North Atlantic waters after the opening of the Fram Strait during the early Miocene. (D) Sediments of the uppermost interval indicate a phase of extreme oxic conditions, and a well‐ventilated environment, which occurred after the complete opening of the Fram Strait. Importantly, and in contrast with classical postrifting thermal subsidence models for passive margins, our data suggest that sediment erosion and/or nondeposition that generated the hiatus was likely due to a progressive shoaling of the Lomonosov Ridge. A shallow water setting both before and after the hiatus suggests that the Lomonosov Ridge remained at or near sea level for the duration of the gap in the sedimentary record. Interacting sea level changes and/or tectonic activity (possibly uplift) must be invoked as possible causes for such a long hiatus.

An Arctic Ocean freshwater event as the trigger of the Younger Dryas stadial? Answers from Arctic deep-sea sediment cores

NASA Astrophysics Data System (ADS)

Spielhagen, Robert F.

2017-04-01

At ca. 12.8-11.5 ka the northern hemisphere climate experienced a dramatic fall-back to quasi-glacial conditions. Since the late 1980s, a major meltwater ejection to the North Atlantic through the Gulf of St.Lawrence was considered the most likely trigger for this "Younger Dryas event". It may have caused a slowdown of the Atlantic meridional overturning circulation (AMOC) and a diminished heat transport to the northern latitudes. However, field evidence from the potential meltwater route in North America has been discussed controversially in the last years, and the detection of a freshwater signal in marine sediments off the St.Lawrence river rendered difficult. More recently, the idea of an "Arctic route" of meltwater originating from proglacial lake Agassiz was put forward (Tarasov & Peltier, Nature 2005) and has gained further attraction through evidence from radiogenic isotopes (Not and Hillaire-Marcel; Nature Comm., 2012) and through modelling results of Condron and Winsor (PNAS, 2012) which showed that only a freshwater outflow through Fram Strait was capable of triggering a climate perturbation like the Younger Dryas. Here I present a review of isotopic records from the Arctic Ocean, the Fram Strait, and the Greenland Sea in search of evidence for a strong freshwater event in the Arctic Ocean at the onset of the Younger Dryas, supporting an Arctic origin of the trigger. A number of Arctic cores show a light planktic oxygen isotope spike at 13 ka. For several of them the age model is detailed enough to exclude a confusion with other deglacial spikes. On the central Arctic Lomonosov Ridge there is even evidence for a diminshed intermediate/bottom water circulation immediately following the freshwater event. On the other hand, there are many records which do not show a meltwater spike in the critical time interval, most likely because of low temporal resolution, a thick ice cover and/or a habitat change of the planktic foraminifers. The largest uncertainty is introduced by arguable reservoir corrections which may need to be applied to radiocarbon datings from the last glacial termination. Nevertheless, since isotopic evidence for a freshwater event at ca. 13 ka is found also in the Greenland Sea, support for an "Arctic trigger" weakening the AMOC is accumulating and may add to the establishment of a new paradigm for the origin of the Younger Dryas cold event.

Arctic Ocean Pathways in the 21st century

NASA Astrophysics Data System (ADS)

Aksenov, Yevgeny; van Gennip, Simon J.; Kelly, Stephen J.; Popova, Ekaterina E.; Yool, Andrew

2017-04-01

In the last three decades, changes in the Arctic environment have been occurring at an increasing rate. The opening up of large areas of previously sea ice-covered ocean affects the marine environment with potential impacts on Arctic ecosystems, including through changes in Arctic access, industries and societies. Changes to sea ice and surface winds result in large-scale shifts in ocean circulation and oceanic pathways. This study presents a high-resolution analysis of the projected ocean circulation and pathways of the Arctic water masses across the 21st century. The analysis is based on an eddy-permitting high-resolution global simulation of the ocean general circulation model NEMO (Nucleus for European Modelling of the Ocean) at the 1/4-degree horizontal resolution. The atmospheric forcing is from HadGEM2-ES model output from IPCC Assessment Report 5 (AR5) simulations performed for Coupled Model Intercomparison Project 5 (CMIP5), and follow the Representative Concentration Pathway 8.5 (RCP8.5) scenario. During the 21st century the AO experiences a significant warming, with sea surface temperature increased by in excess of 4 deg. C. Annual mean Arctic sea ice thickness drops to less than 0.5m, and the Arctic Ocean is ice-free in summer from the mid-century. We use an off-line tracer technique to investigate Arctic pathways of the Atlantic and Pacific waters (AW and PW respectively) under this future climate. The AW tracers have been released in the eastern Fram Strait and in the western Barents Sea, whereas the PW tracer has been seeded in the Bering Strait. In the second half of the century the upper 1000 m ocean circulation shows a reduction in the eastward AW flow along the continental slopes towards the Makarov and Canada basins and a deviation of the PW flow away from the Beaufort Sea towards the Siberian coast. Strengthening of Arctic boundary current and intensification of the cyclonic gyre in the Nansen basin of the Arctic Ocean is accompanied by weakening of the current and an anti-cyclonic gyre spin-up in the Makarov Basin. This presents a shift of the Arctic circulation "dipole" and of the Transpolar Drift, with the consequence that the PW flow towards Fram Strait is significantly reduced by the end of the century, weakening the Pacific-Atlantic connection via the Arctic Ocean, and reducing the Arctic freshwater outflow into the North Atlantic. Examination of the simulations suggests that these circulation changes are primarily due to the shift in the wind.

Locating hazardous gas leaks in the atmosphere via modified genetic, MCMC and particle swarm optimization algorithms

NASA Astrophysics Data System (ADS)

Wang, Ji; Zhang, Ru; Yan, Yuting; Dong, Xiaoqiang; Li, Jun Ming

2017-05-01

Hazardous gas leaks in the atmosphere can cause significant economic losses in addition to environmental hazards, such as fires and explosions. A three-stage hazardous gas leak source localization method was developed that uses movable and stationary gas concentration sensors. The method calculates a preliminary source inversion with a modified genetic algorithm (MGA) and has the potential to crossover with eliminated individuals from the population, following the selection of the best candidate. The method then determines a search zone using Markov Chain Monte Carlo (MCMC) sampling, utilizing a partial evaluation strategy. The leak source is then accurately localized using a modified guaranteed convergence particle swarm optimization algorithm with several bad-performing individuals, following selection of the most successful individual with dynamic updates. The first two stages are based on data collected by motionless sensors, and the last stage is based on data from movable robots with sensors. The measurement error adaptability and the effect of the leak source location were analyzed. The test results showed that this three-stage localization process can localize a leak source within 1.0 m of the source for different leak source locations, with measurement error standard deviation smaller than 2.0.

Phase diagram of pressure-induced superconductor β-(BDA-TTP)2MX4 (M=Fe, Ga and X=Cl, Br) with localized magnetic moments

NASA Astrophysics Data System (ADS)

Choi, E. S.; Graf, D.; Tokumoto, T.; Brooks, J. S.; Yamada, Jun-Ichi

2007-03-01

We have investigated transport and magnetization properties of β-(BDA-TTP)2MX4 (M=Fe, Ga and X=Cl, Br) as a function of pressure, temperature and magnetic field. The title material undergoes metal-insulator transitions above 100 K at ambient pressure. The insulating phase is suppressed with pressure and superconductivity eventually appears above Pc= 4.5 kbar (X=Cl) and 13 kbar (X=Br). The general temperature-pressure (TP) phase diagram is similar each other, while higher pressure is required for X=Br compounds to suppress the insulating state and induce the superconductivity. Pressure dependent DC magnetization studies on β-(BDA-TTP)2FeCl4 compound revealed that the AFM ordering persist well above Pc. In spite of similarity of phase diagram between M=Fe and M=Ga compounds, magnetoresistance results show distinct behaviors, which indicates the magnetic interaction with the conduction electrons are still effective. The comparison between X=Cl and X=Br compounds suggests the anion-size effect rather than the existence of localized magnetic moments plays more important role in determining the ground state.

Gold-mercaptopropionic acid-polyethylenimine composite based DNA sensor for early detection of rheumatic heart disease.

PubMed

Singh, Swati; Kaushal, Ankur; Khare, Shashi; Kumar, Pradeep; Kumar, Ashok

2014-07-21

The first gold-mercaptopropionic acid-polyethylenimine composite based electrochemical DNA biosensor was fabricated for the early detection of Streptococcus pyogenes infection in humans causing rheumatic heart disease (heart valve damage). No biosensor is available for the detection of rheumatic heart disease (RHD). Therefore, the mga gene based sensor was developed by the covalent immobilization of a 5'-carboxyl modified single stranded DNA probe onto the gold composite electrode. The immobilized probe was hybridized with the genomic DNA (G-DNA) of S. pyogenes from throat swabs and the electrochemical response was measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance (EI). Covalent immobilization of the probe onto the gold composite and its hybridization with G-DNA was characterized by FTIR and SEM. The sensitivity of the sensor was 110.25 μA cm(-2) ng(-1) with DPV and the lower limit of detection was 10 pg per 6 μL. The sensor was validated with patient throat swab samples and results were compared with available methods. The sensor is highly specific to S. pyogenes and can prevent damage to heart valves by the early detection of the infection in only 30 min.

Metal ion displacements in noncentrosymmetric chalcogenides La{sub 3}Ga{sub 1.67}S{sub 7}, La{sub 3}Ag{sub 0.6}GaCh{sub 7} (Ch=S, Se), and La{sub 3}MGaSe{sub 7} (M=Zn, Cd)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Iyer, Abishek K.; Yin, Wenlong; Institute of Chemical Materials, China Academy of Engineering Physics, Mianyang 621900

The quaternary Ga-containing chalcogenides La{sub 3}Ag{sub 0.6}GaS{sub 7}, La{sub 3}Ag{sub 0.6}GaSe{sub 7}, La{sub 3}ZnGaSe{sub 7}, and La{sub 3}CdGaSe{sub 7}, as well as the related ternary chalcogenide La{sub 3}Ga{sub 1.67}S{sub 7}, were prepared by reactions of the elements at 950 °C. They adopt noncentrosymmetric hexagonal structures (space group P6{sub 3}, Z=2) with cell parameters (a=10.2 Å, c=6.1 Å for the sulfides; a=10.6 Å, c=6.4 Å for the selenides) that are largely controlled by the geometrical requirements of one-dimensional stacks of Ga-centered tetrahedra separated by the La atoms. Among these compounds, which share the common formulation La{sub 3}M{sub 1–x}GaCh{sub 7} (M=Ga, Ag,more » Zn, Cd; Ch=S, Se), the M atoms occupy sites within a stacking of trigonal antiprisms formed by Ch atoms. The location of the M site varies between extremes with trigonal antiprismatic (CN6) and trigonal planar (CN3) geometry. Partial occupation of these sites and intermediate ones accounts for the considerable versatility of these structures and the occurrence of large metal displacement parameters. The site occupations can be understood in a simple way as being driven by the need to satisfy appropriate bond valence sums for both the M and Ch atoms. Band structure calculations rationalize the substoichiometry observed in the Ag-containing compounds (La{sub 3}Ag{sub 0.6}GaS{sub 7}, La{sub 3}Ag{sub 0.6}GaSe{sub 7}) as a response to overbonding. X-ray photoelectron spectroscopy supports the presence of monovalent Ag atoms in these compounds, which are not charge-balanced. - Graphical abstract: Partial occupation of metal atoms in multiple sites accounts for versatility in Ga-containing chalcogenides La{sub 3}M{sub 1–x}GaCh{sub 7} with noncentrosymmetric hexagonal structures. - Highlights: • La{sub 3}M{sub 1–x}GaCh{sub 7} (M =Ga, Ag, Zn, Cd; Ch =S, Se) adopt related hexagonal structures. • Large displacements of M atoms originate from partial occupation of multiple sites. • Bond valence sum arguments give a simple explanation for site preference. • XPS studies confirm presence of monovalent Ag in La{sub 3}Ag{sub 0.6}GaCh{sub 7}. • Substoichiometry in La{sub 3}Ag{sub 0.6}GaCh{sub 7} avoids occupation of Ag–Ch antibonding levels.« less

Inter-annual variability of transparent exopolymer particles in the Arctic Ocean reveals high sensitivity to ecosystem changes.

PubMed

Engel, Anja; Piontek, Judith; Metfies, Katja; Endres, Sonja; Sprong, Pim; Peeken, Ilka; Gäbler-Schwarz, Steffi; Nöthig, Eva-Maria

2017-06-23

Transparent exopolymer particles (TEP) are a class of marine gel particles and important links between surface ocean biology and atmospheric processes. Derived from marine microorganisms, these particles can facilitate the biological pumping of carbon dioxide to the deep sea, or act as cloud condensation and ice nucleation particles in the atmosphere. Yet, environmental controls on TEP abundance in the ocean are poorly known. Here, we investigated some of these controls during the first multiyear time-series on TEP abundance for the Fram Strait, the Atlantic gateway to the Central Arctic Ocean. Data collected at the Long-Term Ecological Research observatory HAUSGARTEN during 2009 to 2014 indicate a strong biological control with highest abundance co-occurring with the prymnesiophyte Phaeocystis pouchetii. Higher occurrence of P. pouchetii in the Arctic Ocean has previously been related to northward advection of warmer Atlantic waters, which is expected to increase in the future. Our study highlights the role of plankton key species in driving climate relevant processes; thus, changes in plankton distribution need to be accounted for when estimating the ocean's biogeochemical response to global change.

Extreme diversity in the songs of Spitsbergen's bowhead whales.

PubMed

Stafford, K M; Lydersen, C; Wiig, Ø; Kovacs, K M

2018-04-01

Almost all mammals communicate using sound, but few species produce complex songs. Two baleen whales sing complex songs that change annually, though only the humpback whale ( Megaptera novaeangliae ) has received much research attention. This study focuses on the other baleen whale singer, the bowhead whale ( Balaena mysticetus ). Members of the Spitsbergen bowhead whale population produced 184 different song types over a 3-year period, based on duty-cycled recordings from a site in Fram Strait in the northeast Atlantic. Distinct song types were recorded over short periods, lasting at most some months. This song diversity could be the result of population expansion, or immigration of animals from other populations that are no longer isolated from each other by heavy sea ice. However, this explanation does not account for the within season and annual shifting of song types. Other possible explanations for the extraordinary diversity in songs could be that it results either from weak selection pressure for interspecific identification or for maintenance of song characteristics or, alternatively, from strong pressure for novelty in a small population. © 2018 The Authors.

CoNNeCT Antenna Positioning System Dynamic Simulator Modal Model Correlation

NASA Technical Reports Server (NTRS)

Jones, Tevor M.; McNelis, Mark E.; Staab, Lucas D.; Akers, James C.; Suarez, Vicente

2012-01-01

The National Aeronautics and Space Administration (NASA) developed an on-orbit, adaptable, Software Defined Radios (SDR)/Space Telecommunications Radio System (STRS)-based testbed facility to conduct a suite of experiments to advance technologies, reduce risk, and enable future mission capabilities on the International Space Station (ISS). The Communications, Navigation, and Networking reConfigurable Testbed (CoNNeCT) Project will provide NASA, industry, other Government agencies, and academic partners the opportunity to develop and field communications, navigation, and networking technologies in both the laboratory and space environment based on reconfigurable, software-defined radio platforms and the STRS Architecture. The CoNNeCT Payload Operations Nomenclature is "SCAN Testbed," and this nomenclature will be used in all ISS integration, safety, verification, and operations documentation. The SCAN Testbed (payload) is a Flight Releasable Attachment Mechanism (FRAM) based payload that will launch aboard the Japanese H-II Transfer Vehicle (HTV) Multipurpose Exposed Pallet (EP-MP) to the International Space Station (ISS), and will be transferred to the Express Logistics Carrier 3 (ELC3) via Extravehicular Robotics (EVR). The SCAN Testbed will operate on-orbit for a minimum of two years.

Changes in compensatory eye movements associated with simulated stimulus conditions of spaceflight

NASA Technical Reports Server (NTRS)

Harm, Deborah L.; Zografos, Linda M.; Skinner, Noel C.; Parker, Donald E.

1993-01-01

Compensatory vertical eye movement gain (CVEMG) was recorded during pitch oscillation in darkness before, during, and immediately after exposures to the stimulus rearrangement produced by the Preflight Adaptation Trainer (PAT) Tilt-Translation Device (TTD). The TTD is designed to elicit adaptive responses that are similar to those observed in microgravity-adapted astronauts. The data from Experiment 1 yielded a statistically significant CVEMG decrease following 15 min of exposure to a stimulus rearrangement condition where the phase angle between subject pitch tilt and visual scene translation was 270 deg; statistically significant gain decreases were not observed following exposures either to a condition where the phase angle between subject pitch and scene translation was 90 deg or to a no-stimulus-rearrangement condition. Experiment 2 replicated the 270-deg-phase condition from Experiment 1 and extended the exposure duration from 30 to 45 min. Statistically significant additional changes in CVEMG associated with the increased exposure duration were not observed. The adaptation time constant estimated fram the combined data from Experiments 1 and 2 was 29 min.

CoNNeCT Antenna Positioning System Dynamic Simulator Modal Model Correlation

NASA Technical Reports Server (NTRS)

Jones, Trevor M.; McNelis, Mark E.; Staab, Lucas D.; Akers, James C.; Suarez, Vicente J.

2012-01-01

The National Aeronautics and Space Administration (NASA) developed an on-orbit, adaptable, Software Defined Radios (SDR)/Space Telecommunications Radio System (STRS)-based testbed facility to conduct a suite of experiments to advance technologies, reduce risk, and enable future mission capabilities on the International Space Station (ISS). The Communications, Navigation, and Networking reConfigurable Testbed (CoNNeCT) Project will provide NASA, industry, other Government agencies, and academic partners the opportunity to develop and field communications, navigation, and networking technologies in both the laboratory and space environment based on reconfigurable, software-defined radio platforms and the STRS Architecture. The CoNNeCT Payload Operations Nomenclature is SCAN Testbed, and this nomenclature will be used in all ISS integration, safety, verification, and operations documentation. The SCAN Testbed (payload) is a Flight Releasable Attachment Mechanism (FRAM) based payload that will launch aboard the Japanese H-II Transfer Vehicle (HTV) Multipurpose Exposed Pallet (EP-MP) to the International Space Station (ISS), and will be transferred to the Express Logistics Carrier 3 (ELC3) via Extravehicular Robotics (EVR). The SCAN Testbed will operate on-orbit for a minimum of two years.

Enhanced energy harvesting in commercial ferroelectric materials

NASA Astrophysics Data System (ADS)

Patel, Satyanarayan; Chauhan, Aditya; Vaish, Rahul

2014-04-01

Ferroelectric materials are used in a number of applications ranging from simple sensors and actuators to ferroelectric random access memories (FRAMs), transducers, health monitoring system and microelectronics. The multiphysical coupling ability possessed by these materials has been established to be useful for energy harvesting applications. However, conventional energy harvesting techniques employing ferroelectric materials possess low energy density. This has prevented the successful commercialization of ferroelectric based energy harvesting systems. In this context, the present study aims at proposing a novel approach for enhanced energy harvesting using commercially available ferroelectric materials. This technique was simulated to be used for two commercially available piezoelectric materials namely PKI-552 and APCI-840, soft and hard lead-zirconate-titanate (PZT) pervoskite ceramics, respectively. It was observed that a maximum energy density of 348 kJm-3cycle-1 can be obtained for cycle parameters of (0-1 ton compressive stress and 1-25 kV.cm-1 electric field) using APCI-840. The reported energy density is several hundred times larger than the maximum energy density reported in the literature for vibration harvesting systems.

Design of the VISITOR Tool: A Versatile ImpulSive Interplanetary Trajectory OptimizeR

NASA Technical Reports Server (NTRS)

Corpaccioli, Luca; Linskens, Harry; Komar, David R.

2014-01-01

The design of trajectories for interplanetary missions represents one of the most complex and important problems to solve during conceptual space mission design. To facilitate conceptual mission sizing activities, it is essential to obtain sufficiently accurate trajectories in a fast and repeatable manner. To this end, the VISITOR tool was developed. This tool modularly augments a patched conic MGA-1DSM model with a mass model, launch window analysis, and the ability to simulate more realistic arrival and departure operations. This was implemented in MATLAB, exploiting the built-in optimization tools and vector analysis routines. The chosen optimization strategy uses a grid search and pattern search, an iterative variable grid method. A genetic algorithm can be selectively used to improve search space pruning, at the cost of losing the repeatability of the results and increased computation time. The tool was validated against seven flown missions: the average total mission (Delta)V offset from the nominal trajectory was 9.1%, which was reduced to 7.3% when using the genetic algorithm at the cost of an increase in computation time by a factor 5.7. It was found that VISITOR was well-suited for the conceptual design of interplanetary trajectories, while also facilitating future improvements due to its modular structure.

General virial theorem for modified-gravity MOND

NASA Astrophysics Data System (ADS)

Milgrom, Mordehai

2014-01-01

An important and useful relation is known to hold in two specific MOND theories. It pertains to low-acceleration, isolated systems of pointlike masses, mp, at positions rp, subject to gravitational forces Fp. It reads ∑prp·Fp=-(2/3)(Ga0)1/2 [(∑pmp)3/2-∑p mp3/2]; a0 is the MOND acceleration constant. Here I show that this relation holds in the nonrelativistic limit of any modified-gravity MOND theory. It follows from only the basic tenets of MOND, which include departure from standard dynamics at accelerations below a0, and space-time scale invariance in the nonrelativistic, low-acceleration limit. This implies space-dilatation invariance of the static, gravitational-field equations, which, in turn, leads to the above point-mass virial relation. Thus, the various MOND predictions and tests based on this relation hold in any modified-gravity MOND theory. Since we do not know that any of the existing MOND theories point in the right direction, it is important to identify such predictions that hold in a much larger class of theories. Among these predictions are the MOND two-body force for arbitrary masses, and a general mass-velocity-dispersion relation of the form σ2=(2/3)(MGa0)1/2[1-∑p(mp/M)3/2], where M = ∑p mp.

Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome

PubMed Central

Saeki, Yasushi; Kudo, Tai; Sone, Takayuki; Kikuchi, Yoshiko; Yokosawa, Hideyoshi; Toh-e, Akio; Tanaka, Keiji

2009-01-01

Recruitment of substrates to the 26S proteasome usually requires covalent attachment of the Lys48-linked polyubiquitin chain. In contrast, modifications with the Lys63-linked polyubiquitin chain and/or monomeric ubiquitin are generally thought to function in proteasome-independent cellular processes. Nevertheless, the ubiquitin chain-type specificity for the proteasomal targeting is still poorly understood, especially in vivo. Using mass spectrometry, we found that Rsp5, a ubiquitin-ligase in budding yeast, catalyzes the formation of Lys63-linked ubiquitin chains in vitro. Interestingly, the 26S proteasome degraded well the Lys63-linked ubiquitinated substrate in vitro. To examine whether Lys63-linked ubiquitination serves in degradation in vivo, we investigated the ubiquitination of Mga2-p120, a substrate of Rsp5. The polyubiquitinated p120 contained relatively high levels of Lys63-linkages, and the Lys63-linked chains were sufficient for the proteasome-binding and subsequent p120-processing. In addition, Lys63-linked chains as well as Lys48-linked chains were detected in the 26S proteasome-bound polyubiquitinated proteins. These results raise the possibility that Lys63-linked ubiquitin chain also serves as a targeting signal for the 26S proteaseome in vivo. PMID:19153599

Facial Emotion Recognition System – A Machine Learning Approach

NASA Astrophysics Data System (ADS)

Ramalingam, V. V.; Pandian, A.; Jayakumar, Lavanya

2018-04-01

Frown is a medium for people correlation and it could be exercised in multiple real systems. Single crucial stage for frown realizing is to exactly select hysterical aspects. This journal proposed a frown realization scheme applying transformative Particle Swarm Optimization (PSO) based aspect accumulation. This entity initially employs changed LVP, handles crisscross adjacent picture element contrast, for achieving the selective first frown portrayal. Then the PSO entity inserted with a concept of micro Genetic Algorithm (mGA) called mGA-embedded PSO designed for achieving aspect accumulation. This study, the technique subsumes no disposable memory, a little-populace insignificant flock, a latest acceleration that amends with the approach and a sub dimension-based in-depth local frown aspect examines. Assistance of provincial utilization and comprehensive inspection examine structure of alleviating of an immature concurrence complication of conventional PSO. Numerous identifiers are used to diagnose different frown expositions. Stationed on extensive study within and other-sphere pictures from the continued Cohn Kanade and MMI benchmark directory appropriately. Determination of the application exceeds most advanced level PSO variants, conventional PSO, classical GA and alternate relevant frown realization structures is described with powerful limit. Extending our accession to a motion based FER application for connecting patch-based Gabor aspects with continuous data in multi-frames.

Cell cycle phase dependent emergence of thymidylate synthase studied by monoclonal antibody (M-TS-4).

PubMed

Shibui, S; Hoshino, T; Iwasaki, K; Nomura, K; Jastreboff, M M

1989-05-01

A method of identifying thymidylate synthase (TS) at the cellular level was developed using anti-TS monoclonal antibody (M-TS-4), a monoclonal antibody created against purified TS from a HeLa cell line. In HeLa cells and four human glioma cell lines (U-251, U-87, 343-MGA, and SF-188), TS was identified primarily in the cytoplasm. Autoradiographic and flow cytometric studies showed that TS appeared mainly in the G1 phase and subsided early in the S phase; thus, the G1 phase can be divided into TS-positive and -negative fractions. Nuclear TS was not demonstrated unequivocally with M-TS-4, and the relationship between nuclear TS and DNA synthesis could not be determined. Although the percentage of TS-positive cells was larger than the S-phase fraction measured by autoradiography after a pulse of tritiated thymidine or by the immunoperoxidase method using BUdR, the ratios were within a similar range (1.2-1.4) in all cell lines studied. Therefore, the S-phase fraction can be estimated indirectly from the percentage of TS-positive cells measured by M-TS-4. Because the emergence of TS detected by our method is cell cycle dependent, M-TS-4 may be useful for biochemical studies of TS and for cytokinetic analysis.

Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus.

PubMed

Carnicer, Marc; Vieira, Gilles; Brautaset, Trygve; Portais, Jean-Charles; Heux, Stephanie

2016-06-01

The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria into an efficient microbial cell factory. Due to the diversity of chemical and cell properties, an experimental protocol validated on B. methanolicus is needed. Here a systematic evaluation of different techniques for establishing a reliable basis for metabolome investigations is presented. Metabolome analysis was focused on metabolites closely linked with B. methanolicus central methanol metabolism. As an alternative to cold solvent based procedures, a solvent-free quenching strategy using stainless steel beads cooled to -20 °C was assessed. The precision, the consistency of the measurements, and the extent of metabolite leakage from quenched cells were evaluated in procedures with and without cell separation. The most accurate and reliable performance was provided by the method without cell separation, as significant metabolite leakage occurred in the procedures based on fast filtration. As a biological test case, the best protocol was used to assess the metabolome of B. methanolicus grown in chemostat on methanol at two different growth rates and its validity was demonstrated. The presented protocol is a first and helpful step towards developing reliable metabolomics data for thermophilic methylotroph B. methanolicus. This will definitely help for designing an efficient methylotrophic cell factory.

Methylotrophy in the thermophilic Bacillus methanolicus, basic insights and application for commodity production from methanol.

PubMed

Müller, Jonas E N; Heggeset, Tonje M B; Wendisch, Volker F; Vorholt, Julia A; Brautaset, Trygve

2015-01-01

Using methanol as an alternative non-food feedstock for biotechnological production offers several advantages in line with a methanol-based bioeconomy. The Gram-positive, facultative methylotrophic and thermophilic bacterium Bacillus methanolicus is one of the few described microbial candidates with a potential for the conversion of methanol to value-added products. Its capabilities of producing and secreting the commercially important amino acids L-glutamate and L-lysine to high concentrations at 50 °C have been demonstrated and make B. methanolicus a promising target to develop cell factories for industrial-scale production processes. B. methanolicus uses the ribulose monophosphate cycle for methanol assimilation and represents the first example of plasmid-dependent methylotrophy. Recent genome sequencing of two physiologically different wild-type B. methanolicus strains, MGA3 and PB1, accompanied with transcriptome and proteome analyses has generated fundamental new insight into the metabolism of the species. In addition, multiple key enzymes representing methylotrophic and biosynthetic pathways have been biochemically characterized. All this, together with establishment of improved tools for gene expression, has opened opportunities for systems-level metabolic engineering of B. methanolicus. Here, we summarize the current status of its metabolism and biochemistry, available genetic tools, and its potential use in respect to overproduction of amino acids.

Evaluation of Arctic Sea Ice Thickness Simulated by AOMIP Models

NASA Technical Reports Server (NTRS)

Johnson, Mark; Proshutinsky, Andrey; Aksenov, Yevgeny; Nguyen, An T.; Lindsay, Ron; Haas, Christian; Zhang, Jinlun; Diansky, Nimolay; Kwok, Ron; Maslowski, Wieslaw;

Cold-seep ostracods from the western Svalbard margin: direct palaeo-indicator for methane seepage?

NASA Astrophysics Data System (ADS)

Yasuhara, Moriaki; Sztybor, Kamila; Rasmussen, Tine L.; Okahashi, Hisayo; Sato, Runa; Tanaka, Hayato

2018-01-01

Despite their high abundance and diversity, microfossil taxa adapted to a particular chemosynthetic environment have rarely been studied and are therefore poorly known. Here we report on an ostracod species, Rosaliella svalbardensis gen. et sp. nov., from a cold methane seep site at the western Svalbard margin, Fram Strait. The new species shows a distinct morphology, different from other eucytherurine ostracod genera. It has a marked similarity to Xylocythere, an ostracod genus known from chemosynthetic environments of wood falls and hydrothermal vents. Rosaliella svalbardensis is probably an endemic species or genus linked to methane seeps. We speculate that the surface ornamentation of pore clusters, secondary reticulation, and pit clusters may be related to ectosymbiosis with chemoautotrophic bacteria. This new discovery of specialized microfossil taxa is important because they can be used as an indicator species for past and present seep environments (http://zoobank.org/urn:lsid:zoobank.org:pub:6075FF30-29D5-4DAB-9141-AE722CD3A69B).

Initial Results from On-Orbit Testing of the Fram Memory Test Experiment on the Fastsat Micro-Satellite

NASA Technical Reports Server (NTRS)

MacLeond, Todd C.; Sims, W. Herb; Varnavas,Kosta A.; Ho, Fat D.

2011-01-01

The Memory Test Experiment is a space test of a ferroelectric memory device on a low Earth orbit satellite that launched in November 2010. The memory device being tested is a commercial Ramtron Inc. 512K memory device. The circuit was designed into the satellite avionics and is not used to control the satellite. The test consists of writing and reading data with the ferroelectric based memory device. Any errors are detected and are stored on board the satellite. The data is sent to the ground through telemetry once a day. Analysis of the data can determine the kind of error that was found and will lead to a better understanding of the effects of space radiation on memory systems. The test is one of the first flight demonstrations of ferroelectric memory in a near polar orbit which allows testing in a varied radiation environment. The initial data from the test is presented. This paper details the goals and purpose of this experiment as well as the development process. The process for analyzing the data to gain the maximum understanding of the performance of the ferroelectric memory device is detailed.

VizieR Online Data Catalog: New minima timings and RVs for 3 eclipsing binaries (Zasche+, 2017)

NASA Astrophysics Data System (ADS)

Zasche, P.; Jurysek, J.; Nemravova, J.; Uhlar, R.; Svoboda, P.; Wolf, M.; Honkova, K.; Masek, M.; Prouza, M.; Cechura, J.; Korcakova, D.; Slechta, M.

2018-04-01

Spectroscopy was obtained in two observatories. Most of the data points for these systems came from the Ondrejov observatory and its 2 m telescope (resolution R~12500). Additionally, data on BR Ind and some data on QS Aql were obtained with the FEROS instrument mounted on the 2.2 m MPG telescope located in La Silla Observatory in Chile (R~48000). Photometry for these three systems was collected over the time span of 2008 to 2016. Owing to the relatively high brightness of the targets, only rather small telescopes were used for these photometric observations. The system V773 Cas was observed (by one of the authors, PS) with a 34 mm refractor at a private observatory in Brno, Czech Republic, using an SBIG ST-7XME CCD camera. The star QS Aql was monitored (by one of the authors, RU) with a similar instrument at a private observatory in Jilove u Prahy, Czech Republic, using a G2-0402 CCD camera. The only southern star, BR Ind, was observed with the FRAM telescope (Prouza et al. 2010AdAst2010E..31P), installed and operated at the Pierre Auger Observatory at Malargue, Argentina. (2 data files).

Late Eocene to present isotopic (Sr-Nd-Pb) and geochemical evolution of sediments from the Lomonosov Ridge, Arctic Ocean: Implications for continental sources and linkage with the North Atlantic Ocean

NASA Astrophysics Data System (ADS)

Stevenson, Ross; Poirier, André; Véron, Alain; Carignan, Jean; Hillaire-Marcel, Claude

2015-09-01

New geochemical and isotopic (Sr, Nd, Pb) data are presented for a composite sedimentary record encompassing the past 50 Ma of history of sedimentation on the Lomonosov Ridge in the Arctic Ocean. The sampled sediments encompass the transition of the Arctic basin from an enclosed anoxic basin to an open and ventilated oxidized ocean basin. The transition from anoxic basin to open ventilated ocean is accompanied by at least three geochemical and isotopic shifts and an increase in elements (e.g., K/Al) controlled by detrital minerals highlighting significant changes in sediment types and sources. The isotopic compositions of the sediments prior to ventilation are more variable but indicate a predominance of older crustal contributions consistent with sources from the Canadian Shield. Following ventilation, the isotopic compositions are more stable and indicate an increased contribution from younger material consistent with Eurasian and Pan-African crustal sources. The waxing and waning of these sources in conjunction with the passage of water through Fram Strait underlines the importance of the exchange of water mass between the Arctic and North Atlantic Oceans.

Comparison of Envisat ASAR and Submarine Sea Ice Thickness Statistics

NASA Astrophysics Data System (ADS)

Hughes, Nicolas E.; Rodrigues, Joao; Wadhams, Peter

2010-12-01

In April 2004 and March 2007 the Royal Navy sent the submarine HMS Tireless on missions into the Arctic Ocean. On both occasions the submarine traversed the area of remaining multi-year sea ice at latitude 85°N north of Greenland acquiring ice draft measurements using upward-looking sonar. The area is outside of the "Gore Box" used for the release of U.S. Submarine data and was beyond the latitude range of the radar altimeter satellites available at that time. This paper compares ice draft statistics with contemporary data from Envisat ASAR to evaluate the level of correlation between SAR backscatter and sea ice thickness. The decline in sea ice volume over the past decade has predominantly been caused by the loss of old multi-year ice due to increased outflow through Fram Strait. Although Tireless found little decrease in the overall ice thickness between 2004 and 2007, the ice rheology was significantly changed with greatly increased quantities of first- and second-year ice in 2007 than had been encountered in 2004. These are evident in changes to the ice draft probability density functions (PDFs) and the ice appearance as seen by the SAR, and presented here.

A propagating freshwater mode in the Arctic Ocean with multidecadal time scale

NASA Astrophysics Data System (ADS)

Schmith, Torben; Malskær Olsen, Steffen; Margrethe Ringgaard, Ida

2017-04-01

We apply Principal Oscillatory Pattern analysis to the Arctic Ocean fresh water content as simulated in a 500 year long control run with constant preindustrial forcing with the EC-Earth global climate model. Two modes emerge from this analysis. One mode is a standing mode with decadal time scale describing accumulation and release of fresh water in the Beaufort Gyre, known in the literature as the Beaufort Gyre flywheel. In addition, we identify a propagating mode with a time scale around 80 years, propagating along the rim of the Canadian Basin. This mode has maximum variability of the fresh water content in the Transpolar Drift and represents the bulk of the total variability of the fresh water content in the Arctic Ocean and also projects on the fresh water through the Fram Strait. Therefore, potentially, it can introduce a multidecadal variability to the Atlantic meridional overturning circulation. We will discuss the physical origin of this propagating mode. This include planetary-scale internal Rossby waves with multidecadal time scale, due to the slow variation of the Coriolis parameter at these high latitudes, as well as topographic steering of these Rossby waves.

Plasma-Assisted Dry Etching of Ferroelectric Capacitor Modules and Application to a 32M Ferroelectric Random Access Memory Devices with Submicron Feature Sizes

NASA Astrophysics Data System (ADS)

Lee, Sang-Woo; Joo, Suk-Ho; Cho, Sung Lae; Son, Yoon-Ho; Lee, Kyu-Mann; Nam, Sang-Don; Park, Kun-Sang; Lee, Yong-Tak; Seo, Jung-Suk; Kim, Young-Dae; An, Hyeong-Geun; Kim, Hyoung-Joon; Jung, Yong-Ju; Heo, Jang-Eun; Lee, Moon-Sook; Park, Soon-Oh; Chung, U-In; Moon, Joo-Tae

2002-11-01

In the manufacturing of a 32M ferroelectric random access memory (FRAM) device on the basis of 0.25 design rule (D/R), one of the most difficult processes is to pattern a submicron capacitor module while retaining good ferroelectric properties. In this paper, we report the ferroelectric property of patterned submicron capacitor modules with a stack height of 380 nm, where the 100 nm-thick Pb(Zr, Ti)O3 (PZT) films were prepared by the sol-gel method. After patterning, overall sidewall slope was approximately 70° and cell-to-cell node separation was made to be 80 nm to prevent possible twin-bit failure in the device. Finally, several heat treatment conditions were investigated to retain the ferroelectric property of the patterned capacitor. It was found that rapid thermal processing (RTP) treatment yields better properties than conventional furnace annealing. This result is directly related to the near-surface chemistry of the PZT films, as confirmed by X-ray photoelectron spectroscopy (XPS) analysis. The resultant switching polarization value of the submicron capacitor was approximately 30 μC/cm2 measured at 3 V.

The not-so-silent world: Measuring Arctic, Equatorial, and Antarctic soundscapes in the Atlantic Ocean

NASA Astrophysics Data System (ADS)

Haver, Samara M.; Klinck, Holger; Nieukirk, Sharon L.; Matsumoto, Haru; Dziak, Robert P.; Miksis-Olds, Jennifer L.

2017-04-01

Anthropogenic noise in the ocean has been shown, under certain conditions, to influence the behavior and health of marine mammals. Noise from human activities may interfere with the low-frequency acoustic communication of many Mysticete species, including blue (Balaenoptera musculus) and fin whales (B. physalus). This study analyzed three soundscapes in the Atlantic Ocean, from the Arctic to the Antarctic, to document ambient sound. For 16 months beginning in August 2009, acoustic data (15-100 Hz) were collected in the Fram Strait (79°N, 5.5°E), near Ascension Island (8°S, 14.4°W) and in the Bransfield Strait (62°S, 55.5°W). Results indicate (1) the highest overall sound levels were measured in the equatorial Atlantic, in association with high levels of seismic oil and gas exploration, (2) compared to the tropics, ambient sound levels in polar regions are more seasonally variable, and (3) individual elements beget the seasonal and annual variability of ambient sound levels in high latitudes. Understanding how the variability of natural and man-made contributors to sound may elicit differences in ocean soundscapes is essential to developing strategies to manage and conserve marine ecosystems and animals.

Zooplankton in the Arctic outflow

NASA Astrophysics Data System (ADS)

Soloviev, K. A.; Dritz, A. V.; Nikishina, A. B.

2009-04-01

Climate changes in the Arctic cause the changes in the current system that may have cascading effect on the structure of plankton community and consequently on the interlinked and delicately balanced food web. Zooplankton species are by definition incapable to perform horizontal moving. Their transport is connected with flowing water. There are zooplankton species specific for the definite water masses and they can be used as markers for the different currents. That allows us to consider zooplankton community composition as a result of water mixing in the studied area. Little is known however about the mechanisms by which spatial and temporal variability in advection affect dynamics of local populations. Ice conditions are also very important in the function of pelagic communities. Melting time is the trigger to all "plankton blooming" processes, and the duration of ice-free conditions determines the food web development in the future. Fram Strait is one of the key regions for the Arctic: the cold water outflow comes through it with the East Greenland Current and meets warm Atlantic water, the West Spitsbergen Current, producing complicated hydrological situation. During 2007 and 2008 we investigated the structure functional characteristics of zooplankton community in the Fram Strait region onboard KV "Svalbard" (April 2007, April and May 2008) and RV "Jan Mayen" (May 2007, August 2008). This study was conducted in frame of iAOOS Norway project "Closing the loop", which, in turn, was a part of IPY. During this cruises multidisciplinary investigations were performed, including sea-ice observations, CTD and ADCP profiling, carbon flux, nutrients and primary production measurements, phytoplankton sampling. Zooplankton was collected with the Hydro-Bios WP2 net and MultiNet Zooplankton Sampler, (mouth area 0.25 m2, mesh size 180 um).Samples were taken from the depth strata of 2000-1500, 1500-1000, 1000-500,500-200, 200-100, 100-60, 60-30, 30-0 m. Gut fluorescence content were measured in dominant species to investigate effect of Chl a concentration and phytoplankton composition on ingestion rate. Egg production experiments were carried out under different food conditions. Rare deep water zooplankton species were also investigated to increase our knowledge in the Arctic biodiversity. Copepods Calanus finmarchicus is known as a marker of the Atlantic water mass, Calanus glacialis and Calanus hyperboreus, vice versa, are the coldwater Arctic species. In our study we investigated three Calanus species distribution and analyzed their ecological status. Changes in zooplankton composition results in the alteration of energy transfer within the pelagic food web ("cold" and "warm" scenarios) with potential consequences for growth and survival of seabirds Little Auk (Alle alle) and Black-legged kittiwake (Rissa tridactyla). We discuss the advection effect on the zooplankton community, compare the population development phases with phytoplankton bloom phases (match-mismatch), estimate grazing impact on phytoplankton community and consider different life strategies for the three different Calanus species.

Reconstruction of sea-ice cover and primary production on the East Greenland Shelf (73°N) during the last 5200 years

NASA Astrophysics Data System (ADS)

Kolling, Henriette Marie; Stein, Rüdiger; Fahl, Kirsten; Perner, Kerstin; Moros, Matthias

2016-04-01

Over the last decades the extent and thickness of Arctic sea ice has changed dramatically and much more rapidly than predicted by climate models. Thus, high-resolution sea-ice reconstructions from pre-anthropogenic times are useful and needed in order to better understand the processes controlling the natural sea-ice variability. Here, we present the first high-resolution biomarker (IP25, sterols) approach over the last 5.2 ka from the East Greenland Shelf (for background about the biomarker approach see Belt et al., 2007; Müller et al., 2009, 2011). This area is highly sensitive to sea-ice changes, as it underlies the pathway of the East Greenland Current, the main exporter of Arctic freshwater and sea ice that affects the environmental conditions on the East Greenland Shelf and deep-water formation/ convection in the Northern North Atlantic. After rather stable sea-ice conditions in the mid-Holocene we found a strong increase in sea ice, cumulating around 1.5 ka and associated with the Neoglacial cooling. The general trend especially during the last 1ka is interrupted by several short-lived events such as the prominent Medieval Warm Period and Little Ice Age, characterized by minimum and maximum sea-ice extent, respectively. Using a spectral analysis, we could identify several cyclicites, e.g. a 45-year cyclicity for cold events. A comparison to similar records from the eastern Fram Strait revealed a slight time lag in the onset of the Neoglacial, but also suggesting the direct link of the East Greenland Shelf area to the Arctic sea-ice/freahwater outflow. A comparison of the biomarker data with a new foraminiferal record obtained from the same site (Perner et al., 2015) suggests that IP25 and foraminifera assemblages are probably controlled by rather different processes within the oceanographic systems, such as the sea-ice conditions and, for the foraminifera, water-mass changes and nutrient supply. References: Belt. S.T., Massé, G., Rowland, S.J., Poulin, M., Michel, C., LeBlanc B., 2007. A novel chemical fossil of palaeo sea ice: IP25. Organic Geochemistry 38 (2007) 16-27 Müller, J., Massé, G., Stein, R., Belt, S.T., 2009. Variability of sea-ice conditons in the Fram Strait over the past 30,000 years. Nature Geoscience Vol 2 (2009), 772-776 Müller, J., Wagner, A., Fahl, K., Stein, R., Prange, M., Lohmann, G., 2011. Towards quantitative sea ice reconstructions in the northern North Atlantic: A combines biomarker and numerical modelling approach. East and Planetrary Science Letters 306 (2011) 137-148 Perner, K., Moros, M., Lloyd, J.M., Jansen, E., Stein, R., 2015. Mid to late Holocene strengthening of the East Greenland Current linked to warm subsurface Atlantic water. Quaternary Science Reviews 129 (2015) 296-307

Late Quaternary sea-ice history of northern Fram Strait/Arctic Ocean

NASA Astrophysics Data System (ADS)

Kremer, Anne; Stein, Rüdiger; Fahl, Kirsten; Matthießen, Jens; Forwick, Matthias; O'Regan, Matt

2016-04-01

One of the main characteristics of the Arctic Ocean is its seasonal to perennial sea-ice cover. Variations of sea-ice conditions affect the Earth's albedo, primary production, rate of deep-water etc.. During the last decades, a drastic decrease in sea ice has been recorded, and the causes of which, i.e., natural vs. anthropogenic forcings, and their relevance within the global climate system, are subject of intense scientific and societal debate. In this context, records of past sea-ice conditions going beyond instrumental records are of major significance. These records may help to better understand the processes controlling natural sea-ice variability and to improve models for forecasts of future climatic conditions. During RV Polarstern Cruise PS92 in summer 2015, a 860 cm long sediment core (PS92/039-2) was recovered from the eastern flank of Yermak Plateau north of the Svalbard archipelago (Peeken, 2015). Based on a preliminary age model, this sediment core probably represents the time interval from MIS 6 to MIS 1. This core, located close to the modern summer ice edge, has been selected for reconstruction of past Arctic sea-ice variability based on specific biomarkers. In this context, we have determined the ice-algae-derived sea-ice proxy IP25 (Belt et al., 2007), in combination with other biomarkers indicative for open-water conditions (cf., Müller et al., 2009, 2011). Furthermore, organic carbon fluxes were differentiated using specific biomarkers indicative for marine primary production (brassicasterol, dinosterol) and terrigenous input (campesterol, β-sitosterol). In this poster, preliminary results of our organic-geochemical and sedimentological investigations are presented. Distinct fluctuations of these biomarkers indicate several major, partly abrupt changes in sea-ice cover in the Yermak Plateau area during the late Quaternary. These changes are probably linked to changes in the inflow of Atlantic Water along the western coastline of Svalbard into the Arctic Ocean. Furthermore, the repetitive advance and retreat of the Svalbard Barents Sea Ice Sheet might have influenced the terrigenous input and the environmental setting north of Svalbard, as reflected in the sediment composition of Core PS92/039-2. References Belt, S.T., Massé, G., Rowland, S.J., Poulin, M., Michel, C., LeBlanc, B., 2007. A novel chemical fossil of paleo sea ice: IP25. Organic Geochemistry 38, 16-27. Müller, J., Massé, G., Stein, R., Belt, S.T., 2009. Variability of sea-ice conditions in the Fram Strait over the past 30,000 years. Nature Geoscience 2 (11), 772-776. Müller, J., Wagner, A., Fahl, K., Stein, R., Prange, M., Lohmann, G., 2011. Towards quantitative sea ice reconstructions in the northern North Atlantic: a combined biomarker and numerical modelling approach. Earth and Planetary Science Letters 306 (3,4), 137-148. Peeken, I. (Ed.), 2015. Cruise report of Arctic Expedition PS92: TRANSSIZ Cruise from Bremerhaven to Longyearbyen (19.05.2015 - 28.06.2015), in preparation.

Integrated analysis of transcriptome and lipid profiling reveals the co-influences of inositol-choline and Snf1 in controlling lipid biosynthesis in yeast.

PubMed

Chumnanpuen, Pramote; Zhang, Jie; Nookaew, Intawat; Nielsen, Jens

2012-07-01

In the yeast Saccharomyces cerevisiae many genes involved in lipid biosynthesis are transcriptionally controlled by inositol-choline and the protein kinase Snf1. Here we undertook a global study on how inositol-choline and Snf1 interact in controlling lipid metabolism in yeast. Using both a reference strain (CEN.PK113-7D) and a snf1Δ strain cultured at different nutrient limitations (carbon and nitrogen), at a fixed specific growth rate of 0.1 h(-1), and at different inositol choline concentrations, we quantified the expression of genes involved in lipid biosynthesis and the fluxes towards the different lipid components. Through integrated analysis of the transcriptome, the lipid profiling and the fluxome, it was possible to obtain a high quality, large-scale dataset that could be used to identify correlations and associations between the different components. At the transcription level, Snf1 and inositol-choline interact either directly through the main phospholipid-involving transcription factors (i.e. Ino2, Ino4, and Opi1) or through other transcription factors e.g. Gis1, Mga2, and Hac1. However, there seems to be flux regulation at the enzyme levels of several lipid involving enzymes. The analysis showed the strength of using both transcriptome and lipid profiling analysis for mapping the co-influence of inositol-choline and Snf1 on phospholipid metabolism.

Risk Assessment of Growth Hormones and Antimicrobial Residues in Meat

PubMed Central

Jeong, Sang-Hee; Kang, Daejin; Lim, Myung-Woon; Kang, Chang Soo

2010-01-01

Growth promoters including hormonal substances and antibiotics are used legally and illegally in food producing animals for the growth promotion of livestock animals. Hormonal substances still under debate in terms of their human health impacts are estradiol-17β, progesterone, testosterone, zeranol, trenbolone, and melengestrol acetate (MGA) . Many of the risk assessment results of natural steroid hormones have presented negligible impacts when they are used under good veterinary practices. For synthetic hormonelike substances, ADIs and MRLs have been established for food safety along with the approval of animal treatment. Small amounts of antibiotics added to feedstuff present growth promotion effects via the prevention of infectious diseases at doses lower than therapeutic dose. The induction of antimicrobial resistant bacteria and the disruption of normal human intestinal flora are major concerns in terms of human health impact. Regulatory guidance such as ADIs and MRLs fully reflect the impact on human gastrointestinal microflora. However, before deciding on any risk management options, risk assessments of antimicrobial resistance require large-scale evidence regarding the relationship between antimicrobial use in food-producing animals and the occurrence of antimicrobial resistance in human pathogens. In this article, the risk profiles of hormonal and antibacterial growth promoters are provided based on recent toxicity and human exposure information, and recommendations for risk management to prevent human health impacts by the use of growth promoters are also presented. PMID:24278538

Findings from the 2009 EBRI/MGA Consumer Engagement in Health Care Survey.

PubMed

Fronstin, Paul

2009-12-01

FIFTH ANNUAL SURVEY: This Issue Brief presents findings from the 2009 EBRI/MGA Consumer Engagement in Health Care Survey, which provides nationally representative data regarding the growth of consumer-driven health plans (CDHPs) and high-deductible health plans (HDHPs), and the impact of these plans and consumer engagement more generally on the behavior and attitudes of adults with private health insurance coverage. Findings from this survey are compared with four earlier annual surveys. ENROLLMENT LOW BUT GROWING: In 2009, 4 percent of the population was enrolled in a CDHP, up from 3 percent in 2008. Enrollment in HDHPs increased from 11 percent in 2008 to 13 percent in 2009. The 4 percent of the population with a CDHP represents 5 million adults ages 21-64 with private insurance, while the 13 percent with a HDHP represents 16.2 million people. Among the 16.2 million individuals with an HDHP, 38 percent (or 6.2 million) reported that they were eligible for a health savings account (HSA) but did not have such an account. Overall, 11.2 million adults ages 21-64 with private insurance, representing 8.9 percent of that market, were either in a CDHP or were in an HDHP that was eligible for an HSA, but had not opened the account. MORE COST-CONSCIOUS BEHAVIOR: Individuals in CDHPs were more likely than those with traditional coverage to exhibit a number of cost-conscious behaviors. They were more likely to say that they had checked whether the plan would cover care; asked for a generic drug instead of a brand name; talked to their doctor about prescription drug options, other treatments, and costs; asked their doctor to recommend a less costly prescription drug; developed a budget to manage health care expenses; checked prices before getting care; and used an online cost-tracking tool. CDHP MORE ENGAGED IN WELLNESS PROGRAMS: CDHP enrollees were more likely than traditional plan enrollees to report that they had the opportunity to fill out a health risk assessment, whereas they were equally likely to report that they had access to a health promotion program. CDHP enrollees were more likely than traditional plan enrollees to participate when a program was offered. Among those not participating, they did not participate because they could make changes on their own; they lacked time; and they were already healthy. FINANCIAL INCENTIVES MATTER: Financial incentives for healthy behavior mattered more to CDHP enrollees than traditional plan enrollees. Financial incentives were a larger factor for CDHP enrollees than for traditional plan enrollees when it came to participating in wellness programs, choice of doctor, and the use of health information technology, as well as patient engagement using e-mail and the Web. HEALTH STATUS IS BETTER, INCOME HIGHER: Adults in CDHPs were significantly less likely to have a health problem than were adults in HDHPs or traditional plans. Adults in CDHPs and HDHPs were significantly less likely to smoke than were adults in traditional plans, and were significantly more likely to exercise. People in CDHPs were also less likely to be obese compared with adults enrolled in a traditional health plan. Adults in CDHPs were significantly more likely than those with traditional health coverage to have a high household income. CDHP and HDHP enrollees were also more likely than traditional plan enrollees to be highly educated.

Exploring organisational competences in Human Factors and UX project work: managing careers, project tactics and organisational strategy.

PubMed

Furniss, Dominic; Curzon, Paul; Blandford, Ann

2018-06-01

Organisational competence in Human Factors and UX (user experience) has not been looked at before despite its relevance to project success. We define organisational competence as the collective competence of the individuals, bringing together their complementary abilities to deliver an outcome that is typically more than the sum of its parts. Twenty-two UX and Human Factors practitioners were interviewed about their project work in two contrasting domains: web design and safety-critical systems to explore organisational competences. Through doing a FRAM analysis, 29 functions and 6 main areas of competences were identified: the central project process; the process of learning about the problem; maintaining and developing client relations; staff development; evolving practices; and the management of documentation for audit and quality control. These dynamic and situated competences form a web of interactions. Managing competences is essential for project success. Implications for managing careers, project tactics and organisational strategy are discussed. Practitioner Summary: Organisational competences impact how routine and non-routine project work is performed, but these have received little attention in the literature. Six key areas of competences in Human Factors and UX project work were identified from practitioner interviews. Managing combinations of adaptive competences is important for developing careers, project tactics and organisational strategies.

Late summer sea ice segmentation with multi-polarisation SAR features in C- and X-band

NASA Astrophysics Data System (ADS)

Fors, A. S.; Brekke, C.; Doulgeris, A. P.; Eltoft, T.; Renner, A. H. H.; Gerland, S.

2015-09-01

In this study we investigate the potential of sea ice segmentation by C- and X-band multi-polarisation synthetic aperture radar (SAR) features during late summer. Five high-resolution satellite SAR scenes were recorded in the Fram Strait covering iceberg-fast first-year and old sea ice during a week with air temperatures varying around zero degrees Celsius. In situ data consisting of sea ice thickness, surface roughness and aerial photographs were collected during a helicopter flight at the site. Six polarimetric SAR features were extracted for each of the scenes. The ability of the individual SAR features to discriminate between sea ice types and their temporally consistency were examined. All SAR features were found to add value to sea ice type discrimination. Relative kurtosis, geometric brightness, cross-polarisation ratio and co-polarisation correlation angle were found to be temporally consistent in the investigated period, while co-polarisation ratio and co-polarisation correlation magnitude were found to be temporally inconsistent. An automatic feature-based segmentation algorithm was tested both for a full SAR feature set, and for a reduced SAR feature set limited to temporally consistent features. In general, the algorithm produces a good late summer sea ice segmentation. Excluding temporally inconsistent SAR features improved the segmentation at air temperatures above zero degrees Celcius.

'Endurance' Goal Across the Plains

NASA Technical Reports Server (NTRS)

2004-01-01

This mosaic image from the Mars Exploration Rover Opportunity's panoramic camera provides an overview of the rover's drive direction toward 'Endurance Crater,' which is in the upper right corner of image.

The plains appear to be uniform in character from the rovers current position all the way to Endurance Crater. Granules of various sizes blanket the plains. Spherical granules fancifully called blueberries are present some intact and some broken. Larger granules pave the surface, while smaller grains, including broken blueberries, form small dunes. Randomly distributed 1-centimeter (0.4 inch) sized pebbles (as seen just left of center in the foreground of the image) make up a third type of feature on the plains. The pebbles' composition remains to be determined. Scientists plan to examine these in the coming sols.

Examination of this part of Mars by NASA's Mars Global Surveyor orbiter revealed the presence of hematite, which led NASA to choose Meridiani Planum as Opportunity's landing site. The rover science conducted on the plains of Meridiani Planum serves to integrate what the rovers are seeing on the ground with what orbital data have shown.

Opportunity will make stop at a small crater called 'Fram' (seen in the upper left, with relatively large rocks nearby) before heading to the rim of Endurance Crater.

Arctic pathways of Pacific Water: Arctic Ocean Model Intercomparison experiments.

PubMed

Aksenov, Yevgeny; Karcher, Michael; Proshutinsky, Andrey; Gerdes, Rüdiger; de Cuevas, Beverly; Golubeva, Elena; Kauker, Frank; Nguyen, An T; Platov, Gennady A; Wadley, Martin; Watanabe, Eiji; Coward, Andrew C; Nurser, A J George

2016-01-01

Pacific Water (PW) enters the Arctic Ocean through Bering Strait and brings in heat, fresh water, and nutrients from the northern Bering Sea. The circulation of PW in the central Arctic Ocean is only partially understood due to the lack of observations. In this paper, pathways of PW are investigated using simulations with six state-of-the art regional and global Ocean General Circulation Models (OGCMs). In the simulations, PW is tracked by a passive tracer, released in Bering Strait. Simulated PW spreads from the Bering Strait region in three major branches. One of them starts in the Barrow Canyon, bringing PW along the continental slope of Alaska into the Canadian Straits and then into Baffin Bay. The second begins in the vicinity of the Herald Canyon and transports PW along the continental slope of the East Siberian Sea into the Transpolar Drift, and then through Fram Strait and the Greenland Sea. The third branch begins near the Herald Shoal and the central Chukchi shelf and brings PW into the Beaufort Gyre. In the models, the wind, acting via Ekman pumping, drives the seasonal and interannual variability of PW in the Canadian Basin of the Arctic Ocean. The wind affects the simulated PW pathways by changing the vertical shear of the relative vorticity of the ocean flow in the Canada Basin.

Vertically Free-Standing Ordered Pb(Zr0.52Ti0.48)O3 Nanocup Arrays by Template-Assisted Ion Beam Etching

NASA Astrophysics Data System (ADS)

Zhang, Xiaoyan; Tang, Dan; Huang, Kangrong; Hu, Die; Zhang, Fengyuan; Gao, Xingsen; Lu, Xubing; Zhou, Guofu; Zhang, Zhang; Liu, Junming

2016-04-01

In this report, vertically free-standing lead zirconate titanate Pb(Zr0.52Ti0.48)O3 (PZT) nanocup arrays with good ordering and high density (1.3 × 1010 cm-2) were demonstrated. By a template-assisted ion beam etching (IBE) strategy, the PZT formed in the pore-through anodic aluminum oxide (AAO) membrane on the Pt/Si substrate was with a cup-like nanostructure. The mean diameter and height of the PZT nanocups (NCs) was about 80 and 100 nm, respectively, and the wall thickness of NCs was about 20 nm with a hole depth of about 80 nm. Uppermost, the nanocup structure with low aspect ratio realized vertically free-standing arrays when losing the mechanical support from templates, avoiding the collapse or bundling when compared to the typical nanotube arrays. X-ray diffraction (XRD) and Raman spectrum revealed that the as-prepared PZT NCs were in a perovskite phase. By the vertical piezoresponse force microscopy (VPFM) measurements, the vertically free-standing ordered ferroelectric PZT NCs showed well-defined ring-like piezoresponse phase and hysteresis loops, which indicated that the high-density PZT nanocup arrays could have potential applications in ultra-high non-volatile ferroelectric memories (NV-FRAM) or other nanoelectronic devices.

Arctic Sea Ice Export Through Fram Strait and Atmospheric Planetary Waves

NASA Technical Reports Server (NTRS)

Cavalieri, Donald J.; Koblinsky, Chester (Technical Monitor)

2001-01-01

A link is found between the variability of Arctic sea ice export through Ram Strait and the phase of the longest atmospheric planetary wave (zonal wave 1) in SLP for the period 1958-1997. Previous studies have identified a link between From Strait ice export and the North Atlantic Oscillation (NAO), but this link has been described as unstable because of a lack of consistency over time scales longer than the last two decades. Inconsistent and low correlations are also found between From Strait ice export and the Arctic Oscillation (AD) index. This paper shows that the phase of zonal wave 1 explains 60% - 70% of the simulated From Strait ice export variance over the Goodyear period 1958 - 1997. Unlike the NAB and AD links, these high variances are consistent for both the first and second halves of the Goodyear period. This consistency is attributed to the sensitivity of the wave I phase at high latitudes to the presence of secondary low pressure systems in the Barents Sea that serve to drive sea ice southward through From Strait. These results provide further evidence that the phase of zonal wave 1 in SLP at high latitudes drives regional as well as hemispheric low frequency Arctic Ocean and sea ice variability.

APPROACHING CRYOGENIC GE PERFORMANCE WITH PELTIER COOLED CDTE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Khusainov, A. K.; Iwanczyk, J. S.; Patt, B. E.

A new class of hand-held, portable spectrometers based on large area (lcm2) CdTe detectors of thickness up to 3mm has been demonstrated to produce energy resolution of between 0.3 and 0.5% FWHM at 662 keV. The system uses a charge loss correction circuit for improved efficiency, and detector temperature stabilization to ensure consistent operation of the detector during field measurements over a wide range of ambient temperature. The system can operate continuously for up to 8hrs on rechargeable batteries. The signal output from the charge loss corrector is compatible with most analog and digital spectroscopy amplifiers and multi channel analyzers.more » Using a detector measuring 11.2 by 9.1 by 2.13 mm3, we have recently been able to obtain the first wide-range plutonium gamma-ray isotopic analysis with other than a cryogenically cooled germanium spectrometer. The CdTe spectrometer is capable of measuring small plutonium reference samples in about one hour, covering the range from low to high burnup. The isotopic analysis software used to obtain these results was FRAM, Version 4 from LANL. The new spectrometer is expected to be useful for low-grade assay, as well as for some in-situ plutonium gamma-ray isotopics in lieu of cryogenically cooled Ge.« less

Picoeukaryote plankton composition off West Spitsbergen at the entrance to the Arctic Ocean.

PubMed

Kilias, Estelle S; Nöthig, Eva-Maria; Wolf, Christian; Metfies, Katja

2014-01-01

Investigation of marine eukaryotic picoplankton composition is limited by missing morphological features for appropriate identification. Consequently, molecular methods are required. In this study, we used 454-pyrosequencing to study picoplankton communities at four stations in the West Spitsbergen Current (WSC; Fram Strait). High abundances of Micromonas pusilla were detected in the station situated closest to Spitsbergen, as seen in surveys of picoplankton assemblages in the Beaufort Sea. At the other three stations, other phylotypes, affiliating with Phaeocystis pouchetii and Syndiniales in the phylogenetic tree, were present in high numbers, dominating most of them. The picoplankton community structures at three of the stations, all with similar salinity and temperature, were alike. At the fourth station, the influence of the East Spitsbergen Current, transporting cold water from the Barents Sea around Spitsbergen, causes different abiotic parameters that result in a significantly different picoeukaryote community composition, which is dominated by M. pusilla. This observation is particularly interesting with regard to ongoing environmental changes in the Arctic. Ongoing warming of the WSC could convey a new picoplankton assemblage into the Arctic Ocean, which may come to affect the dominance of M. pusilla. © 2014 The Author(s) Journal of Eukaryotic Microbiology © 2014 International Society of Protistologists.

An improved independent component analysis model for 3D chromatogram separation and its solution by multi-areas genetic algorithm.

PubMed

Cui, Lizhi; Poon, Josiah; Poon, Simon K; Chen, Hao; Gao, Junbin; Kwan, Paul; Fan, Kei; Ling, Zhihao

2014-01-01

The 3D chromatogram generated by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) has been researched widely in the field of herbal medicine, grape wine, agriculture, petroleum and so on. Currently, most of the methods used for separating a 3D chromatogram need to know the compounds' number in advance, which could be impossible especially when the compounds are complex or white noise exist. New method which extracts compounds from 3D chromatogram directly is needed. In this paper, a new separation model named parallel Independent Component Analysis constrained by Reference Curve (pICARC) was proposed to transform the separation problem to a multi-parameter optimization issue. It was not necessary to know the number of compounds in the optimization. In order to find all the solutions, an algorithm named multi-areas Genetic Algorithm (mGA) was proposed, where multiple areas of candidate solutions were constructed according to the fitness and distances among the chromosomes. Simulations and experiments on a real life HPLC-DAD data set were used to demonstrate our method and its effectiveness. Through simulations, it can be seen that our method can separate 3D chromatogram to chromatogram peaks and spectra successfully even when they severely overlapped. It is also shown by the experiments that our method is effective to solve real HPLC-DAD data set. Our method can separate 3D chromatogram successfully without knowing the compounds' number in advance, which is fast and effective.

Methylotrophic Bacillus methanolicus Encodes Two Chromosomal and One Plasmid Born NAD+ Dependent Methanol Dehydrogenase Paralogs with Different Catalytic and Biochemical Properties

PubMed Central

Müller, Jonas E. N.; Kupper, Christiane E.; Schneider, Olha; Vorholt, Julia A.; Ellingsen, Trond E.; Brautaset, Trygve

2013-01-01

Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD+-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B. methanolicus strains MGA3 (ATCC53907) and PB1 (NCIMB13113) were sequenced and found to harbor three different putative Mdh encoding genes, each belonging to the type III Fe-NAD+-dependent alcohol dehydrogenases. In each strain, two of these genes are encoded on the chromosome and one on a plasmid; only one chromosomal act gene encoding the previously described activator protein ACT was found. The six Mdhs and the ACT proteins were produced recombinantly in Escherichia coli, purified, and characterized. All Mdhs required NAD+ as cosubstrate, were catalytically stimulated by ACT, exhibited a broad and different substrate specificity range and displayed both dehydrogenase and reductase activities. All Mdhs catalyzed the oxidation of methanol; however the catalytic activity for methanol was considerably lower than for most other alcohols tested, suggesting that these enzymes represent a novel class of alcohol dehydrogenases. The kinetic constants for the Mdhs were comparable when acting as pure enzymes, but together with ACT the differences were more pronounced. Quantitative PCR experiments revealed major differences with respect to transcriptional regulation of the paralogous genes. Taken together our data indicate that the repertoire of methanol oxidizing enzymes in thermotolerant bacilli is larger than expected with complex mechanisms involved in their regulation. PMID:23527128

Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties.

PubMed

Krog, Anne; Heggeset, Tonje M B; Müller, Jonas E N; Kupper, Christiane E; Schneider, Olha; Vorholt, Julia A; Ellingsen, Trond E; Brautaset, Trygve

2013-01-01

Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B. methanolicus strains MGA3 (ATCC53907) and PB1 (NCIMB13113) were sequenced and found to harbor three different putative Mdh encoding genes, each belonging to the type III Fe-NAD(+)-dependent alcohol dehydrogenases. In each strain, two of these genes are encoded on the chromosome and one on a plasmid; only one chromosomal act gene encoding the previously described activator protein ACT was found. The six Mdhs and the ACT proteins were produced recombinantly in Escherichia coli, purified, and characterized. All Mdhs required NAD(+) as cosubstrate, were catalytically stimulated by ACT, exhibited a broad and different substrate specificity range and displayed both dehydrogenase and reductase activities. All Mdhs catalyzed the oxidation of methanol; however the catalytic activity for methanol was considerably lower than for most other alcohols tested, suggesting that these enzymes represent a novel class of alcohol dehydrogenases. The kinetic constants for the Mdhs were comparable when acting as pure enzymes, but together with ACT the differences were more pronounced. Quantitative PCR experiments revealed major differences with respect to transcriptional regulation of the paralogous genes. Taken together our data indicate that the repertoire of methanol oxidizing enzymes in thermotolerant bacilli is larger than expected with complex mechanisms involved in their regulation.

The effect of different conventional methods of extraction on Marantodes pumila var. pumila leaves in relation to free radical scavenging activity

NASA Astrophysics Data System (ADS)

Hamid, Noor Huda Abd; Latip, Jalifah; Markom, Masturah

2018-04-01

Marantodes pumila is one of the important herbs used in traditional medicine. However, there is no report on the optimum method for the preparation of M. pumila extract enriched with bioactive compounds. Therefore, this study aim to establish the suitable method in the preparation of M. pumila extracts enriched with antioxidative compounds. Different conventional extraction methods such as maceration, reflux and Soxhlet in solvents of different polarities i.e. ethanol, 50 % aqueous ethanol and water were employed to prepare extracts. Constituents of each extract were profiled using RP-HPLC followed by the free radical scavenging assay using 2,2 diphenyl-1-picrylhydrazyl (DPPH) to determine the antioxidant activity. Among all extraction methods, maceration in 50 % aqueous ethanol gave the highest total percentage yield (22.41 %) while all extraction methods were able to extract the marker compounds, gallic acid (GA) and methyl gallate (MGA). The free radical scavenging activity of extracts varied from IC50 11.93 µg/mL (50 % aqueous ethanol via reflux) to 64.15 µg/mL (water via maceration). All extracts showed better scavenging activity as compared to synthetic antioxidant, BHT. The variation in the IC50 values is expected due to the presence of different types and/or concentration of bioactive compounds in each extract. This study may provide a preliminary insight for the preparation of standardized extracts of M. pumila enriched with bioactive compounds.

Antenna Pointing Mechanisms for Solar Orbiter High and Medium Gain Antennas

NASA Astrophysics Data System (ADS)

Vazquez, Jorge; Pinto, Inaki; Gabiola, Iker; Ibargoyen, I.; Martin, Fernando

2015-09-01

The ESA Solar Orbiter is an interdisciplinary mission to the Sun. It consists of a single spacecraft which will orbit the Sun in a moderately elliptical orbit, using a suite of advanced Remote-Sensing and In-Situ instruments to perform a detailed observation of the Sun and surrounding space. Sener is contractor for the delivery of the Antennas subsystems.The pointing mechanism from HGAMA is a dual-axes gimbal providing azimuth and elevation steering capability. The azimuth axis is driven by the GHM geared to a rotating bracket which supports the elevation actuator and is linked to the HGAMA boom. Both are based on stepper motors with planetary reducers geared to the corresponding output brackets. An integrated X- band dual axes Rotary Joint Assembly (HGA-RJA) routes the RF energy through the APM in both TX and RX directions. The MGAMA APM is a single-axis gimbal providing elevation steering capability, with one built-in actuator and has been design to share many of the components with the elevation axis from HGAMA APM, including a single axis Rotary Joint Assembly (MGA-RJA).Based on BEPI-Colombo heritage, some aspects of the design have been developed specifically for the SolO mission and are presented in this paper.- High temperature ranges in the APM.- Dedicated output shaft support with dedicated flexible coupling.- High accuracy required, with a potentiometer as coarse sensor and inductosyn for fine positioning.- Elevation twist capsule concept based on spiral configuration.- High solar radiation and contamination requirements.

AUV based study on physical and ecological processes at fronts

NASA Astrophysics Data System (ADS)

Tippenhauer, Sandra; Wulff, Thorben; Von Appen, Wilken-Jon

2017-04-01

Small-scale processes and their effects get more and more attention when it comes to understanding processes and changes in the (Arctic) ocean. Here we present a study on physical processes and ecological responses at submesoscale frontal systems in the Fram Strait investigated using an autonomous underwater vehicle (AUV). The AUV is equipped with physical and biogeochemical sensors such as an acoustic Doppler current profiler, a turbulence probe, a conductivity-temperature-depth probe, and sensors for Oxygen, Nitrate, Chlorophyll a, and photosynthetically active radiation (PAR). The study is designed such that the AUV covers tracks of several kilometers length in cross-frontal direction with the front roughly located in the middle of the track. On its way, the AUV records high-resolution vertical or zigzag profiles of the physical and biogeochemical properties in the upper 50 m which includes the euphotic zone. In both, physical and biogeochemical terms, the measurements revealed a complex structure of the water column. At the fronts the distribution of phytoplankton and nutrients was highly inhomogeneous, possibly due to wind-driven frontogenesis or the growth of mixed layer eddies. To set the observations into a larger context we also examine ship-based and satellite data. We investigate how the observed patterns of the potential vorticity and the biogeochemical properties may be formed and which processes could lead to a smoothing of the observed gradients.

Nanoscale investigation of the piezoelectric properties of perovskite ferroelectrics and III-nitrides

NASA Astrophysics Data System (ADS)

Rodriguez, Brian Joseph

Nanoscale characterization of the piezoelectric and polarization related properties of III-Nitrides by piezoresponse force microscopy (PFM), electrostatic force microscopy (EFM) and scanning Kelvin probe microscopy (SKPM) resulted in the measurement of piezoelectric constants, surface charge and surface potential. Photo-electron emission microscopy (PEEM) was used to determine the local electronic band structure of a GaN-based lateral polarity heterostructure (GaN-LPH). Nanoscale characterization of the imprint and switching behavior of ferroelectric thin films by PFM resulted in the observation of domain pinning, while nanoscale characterization of the spatial variations in the imprint and switching behavior of integrated (111)-oriented PZT-based ferroelectric random access memory (FRAM) capacitors by PFM have revealed a significant difference in imprint and switching behavior between the inner and outer parts of capacitors. The inner regions of the capacitors are typically negatively imprinted and consequently tend to switch back after being poled by a positive bias, while regions at the edge of the capacitors tend to exhibit more symmetric hysteresis behavior. Evidence was obtained indicating that mechanical stress conditions in the central regions of the capacitors can lead to incomplete switching. A combination of vertical and lateral piezoresponse force microscopy (VPFM and LPFM, respectively) has been used to map the out-of-plane and in-plane polarization distribution, respectively, of integrated (111)-oriented PZT-based capacitors, which revealed poled capacitors are in a polydomain state.

Arctic pathways of Pacific Water: Arctic Ocean Model Intercomparison experiments

PubMed Central

Karcher, Michael; Proshutinsky, Andrey; Gerdes, Rüdiger; de Cuevas, Beverly; Golubeva, Elena; Kauker, Frank; Nguyen, An T.; Platov, Gennady A.; Wadley, Martin; Watanabe, Eiji; Coward, Andrew C.; Nurser, A. J. George

2016-01-01

Abstract Pacific Water (PW) enters the Arctic Ocean through Bering Strait and brings in heat, fresh water, and nutrients from the northern Bering Sea. The circulation of PW in the central Arctic Ocean is only partially understood due to the lack of observations. In this paper, pathways of PW are investigated using simulations with six state‐of‐the art regional and global Ocean General Circulation Models (OGCMs). In the simulations, PW is tracked by a passive tracer, released in Bering Strait. Simulated PW spreads from the Bering Strait region in three major branches. One of them starts in the Barrow Canyon, bringing PW along the continental slope of Alaska into the Canadian Straits and then into Baffin Bay. The second begins in the vicinity of the Herald Canyon and transports PW along the continental slope of the East Siberian Sea into the Transpolar Drift, and then through Fram Strait and the Greenland Sea. The third branch begins near the Herald Shoal and the central Chukchi shelf and brings PW into the Beaufort Gyre. In the models, the wind, acting via Ekman pumping, drives the seasonal and interannual variability of PW in the Canadian Basin of the Arctic Ocean. The wind affects the simulated PW pathways by changing the vertical shear of the relative vorticity of the ocean flow in the Canada Basin. PMID:27818853

Vertically Free-Standing Ordered Pb(Zr0.52Ti0.48)O3 Nanocup Arrays by Template-Assisted Ion Beam Etching.

PubMed

Zhang, Xiaoyan; Tang, Dan; Huang, Kangrong; Hu, Die; Zhang, Fengyuan; Gao, Xingsen; Lu, Xubing; Zhou, Guofu; Zhang, Zhang; Liu, Junming

2016-12-01

In this report, vertically free-standing lead zirconate titanate Pb(Zr0.52Ti0.48)O3 (PZT) nanocup arrays with good ordering and high density (1.3 × 10(10) cm(-2)) were demonstrated. By a template-assisted ion beam etching (IBE) strategy, the PZT formed in the pore-through anodic aluminum oxide (AAO) membrane on the Pt/Si substrate was with a cup-like nanostructure. The mean diameter and height of the PZT nanocups (NCs) was about 80 and 100 nm, respectively, and the wall thickness of NCs was about 20 nm with a hole depth of about 80 nm. Uppermost, the nanocup structure with low aspect ratio realized vertically free-standing arrays when losing the mechanical support from templates, avoiding the collapse or bundling when compared to the typical nanotube arrays. X-ray diffraction (XRD) and Raman spectrum revealed that the as-prepared PZT NCs were in a perovskite phase. By the vertical piezoresponse force microscopy (VPFM) measurements, the vertically free-standing ordered ferroelectric PZT NCs showed well-defined ring-like piezoresponse phase and hysteresis loops, which indicated that the high-density PZT nanocup arrays could have potential applications in ultra-high non-volatile ferroelectric memories (NV-FRAM) or other nanoelectronic devices.

Bacterial communities in Arctic first-year drift ice during the winter/spring transition.

PubMed

Eronen-Rasimus, Eeva; Piiparinen, Jonna; Karkman, Antti; Lyra, Christina; Gerland, Sebastian; Kaartokallio, Hermanni

2016-08-01

Horizontal and vertical variability of first-year drift-ice bacterial communities was investigated along a North-South transect in the Fram Strait during the winter/spring transition. Two different developmental stages were captured along the transect based on the prevailing environmental conditions and the differences in bacterial community composition. The differences in the bacterial communities were likely driven by the changes in sea-ice algal biomass (2.6-5.6 fold differences in chl-a concentrations). Copiotrophic genera common in late spring/summer sea ice, such as Polaribacter, Octadecabacter and Glaciecola, dominated the bacterial communities, supporting the conclusion that the increase in the sea-ice algal biomass was possibly reflected in the sea-ice bacterial communities. Of the dominating bacterial genera, Polaribacter seemed to benefit the most from the increase in algal biomass, since they covered approximately 39% of the total community at the southernmost stations with higher (>6 μg l(-1) ) chl-a concentrations and only 9% at the northernmost station with lower chl-a concentrations (<6 μg l(-1) ). The sea-ice bacterial communities also varied between the ice horizons at all three stations and thus we recommend that for future studies multiple ice horizons be sampled to cover the variability in sea-ice bacterial communities in spring. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

Ikaite crystals in melting sea ice - implications for pCO2 and pH levels in Arctic surface waters

NASA Astrophysics Data System (ADS)

Rysgaard, S.; Glud, R. N.; Lennert, K.; Cooper, M.; Halden, N.; Leakey, R. J. G.; Hawthorne, F. C.; Barber, D.

2012-03-01

A major issue of Arctic marine science is to understand whether the Arctic Ocean is, or will be, a source or sink for air-sea CO2 exchange. This has been complicated by the recent discoveries of ikaite (CaCO3·6H2O) in Arctic and Antarctic sea ice, which indicate that multiple chemical transformations occur in sea ice with a possible effect on CO2 and pH conditions in surface waters. Here we report on biogeochemical conditions, microscopic examinations and x-ray diffraction analysis of single crystals from an actively melting 1.7 km2 (0.5-1 m thick) drifting ice floe in the Fram Strait during summer. Our findings show that ikaite crystals are present throughout the sea ice but with larger crystals appearing in the upper ice layers. Ikaite crystals placed at elevated temperatures gradually disintegrated into smaller crystallites and dissolved. During our field campaign in late June, melt reduced the ice flow thickness by ca. 0.2 m per week and resulted in an estimated 1.6 ppm decrease of pCO2 in the ocean surface mixed layer. This corresponds to an air-sea CO2 uptake of 11 mmol m-2 sea ice d-1 or to 3.5 ton km-2 ice floe week-1.

Interpretation of time series (salinity and temperature) layers in North Atlantic from 1950 to 2011

NASA Astrophysics Data System (ADS)

Rubchenia, A.; Popov, A.; Fedorova, A.; Lebedev, N.

2012-04-01

On the basis of long period data series (1950-2011) form various sources (National Oceanographic Data Center (NOAD) (www.nodc.noaa.gov), WOD09 database and data from ARGO project) 10 boxes in North Atlantic were selected. Location of boxes was determined by circulation pattern in North Atlantic and Euro-Arctic Seas. For further analysis two "seasons" was selected: "cold season" (October-May) and "warm season" (June-September). Data verification was made. Analysis of data series clearly show the "Great Salinity Anomaly" (so-called GSA) in 1960-70th, 1980th and 1990th. Trends of salinity and temperature data series were calculated. Spectral analysis allow us to calculate periodicity from 2 to 22 years. Boxes situated in regions with Arctic waters have singularity showed through domination of high frequency oscillation during propagation to South. In Fram Strait salinity fluctuates with periods 9..11 and 20 years, the same period was calculated using temperature data series. In Denmark Strait there are oscillations of temperature with specific period from 4 to 7 years. Range of variability vary. For salinity it is 0.4..4.6 psu, for temperature it is 0.04..5.5C. In salinity data series from boxes with surface Arctic waters noticed clear minimums connected with GSAs. Trends in Denmark Strait and Fram strait in the end of 2000th are negative at different levels. Since 1975 to 2001 salinity near the southern part of Greenland was increased, since 2001 - decreased. But temperature was raised from 0.04 in 1989 to 5.59 in 2010. Thermohaline characteristics of water masses which has Atlantic origin oscillated with period near 20 years. Salinity near Newfoundland was decreased since 2005. In Farrero-Shetland straits salinity trend is positive since at 100m level, Salinity rising from 1970th to 2006 is about 0.3 psu were noticed. Oscillations with period 2..4 years is weak. But at 800m layer salinity oscillations are different, since 1990 there is not significant oscillations at all. Temperature trend at this level is negative since 1950th. Salinity at 100-300 level at Station M area described with negative trend since 1960 to 1993, in both "seasons". Next, up to 2010 salinity is increasing, but in 2011 salinity dramatically decreased. Main oscillations have periods 2..3 years, 4..5 years and 20 years. At 800m level oscillations are very weak. Temperature is increased since 1995 in surface layer and since 2002 in deeper levels. At all levels temperature dramatically decreased after 2010. In central part of Greenland Sea ("Cupola area") dominated oscillations with period 4 years (1950-60th), 5..7 years (1970th) and 9 years (after 1979). In "cold season" oscillation with 11 years traced. Salinity trend is positive at all levels during last 10-15 years. Salinity and temperature were increased at 800m level up to 2006. It could lead to termination of deep water formation. Since 2006 temperature decreased, especially in "cold season". Salinity trend in West Spitsbergen Current is positive since 1996 at surface and sine 1978 at deeper levels. Temperature was increased since 1965 to 2006 in surface layer, but since 2006 in "warm season" temperature is decreasing at all layers. Main oscillations is 4..5 years, 6..7 years and 9..11 years.

Late Pliocene/Pleistocene changes in Arctic sea-ice cover: Biomarker and dinoflagellate records from Fram Strait/Yermak Plateau (ODP Sites 911 and 912)

NASA Astrophysics Data System (ADS)

Stein, Ruediger; Fahl, Kirsten; Matthiessen, Jens

2014-05-01

Sea ice is a critical component in the (global) climate system that contributes to changes in the Earth's albedo (heat reduction) and biological processes (primary productivity), as well as deep-water formation, a driving mechanism for global thermohaline circulation. Thus, understanding the processes controlling Arctic sea ice variability is of overall interest and significance. Recently, a novel and promising biomarker proxy for reconstruction of Arctic sea-ice conditions was developed and is based on the determination of a highly-branched isoprenoid with 25 carbons (IP25; Belt et al., 2007; PIP25 when combined with open-water phytoplankton biomarkers; Müller et al., 2011). Here, we present biomarker data from Ocean Drilling Program (ODP) Sites 911 and 912, recovered from the southern Yermak Plateau and representing information of sea-ice variability, changes in primary productivity and terrigenous input during the last about 3.5 Ma. As Sites 911 and 912 are close to the modern sea-ice edge, their sedimentary records seem to be optimal for studying past variability in sea-ice coverage and testing the applicability of IP25 and PIP25 in older sedimentary sequences. In general, our biomarker records correlate quite well with other climate and sea-ice proxies (e.g., dinoflagellates, IRD, etc.). The main results can be summarized as follows: (1) The novel IP25/PIP25 biomarker approach has potential for semi-quantitative paleo-sea ice studies covering at least the last 3.5 Ma, i.e., the time interval including the onset (intensification) of major Northern Hemisphere Glaciation (NHG). (2) These data indicate that sea ice of variable extent was present in the Fram Strait/southern Yermak Plateau area during most of the time period under investigation. (3) Elevated IP25/PIP25 values indicative for an extended spring sea-ice cover, already occurred between 3.6 and 2.9 Ma, i.e., prior to the onset of major NHG. This may suggest that sea-ice and related albedo effects might have been important for general cooling and ice-sheet build-up. (4) Maxima in sea ice occurred near 3.3, 2.7, 2.1, 1.7 and during the last 1.2 Ma whereas between about 2.6 and 2.2 Ma the sea-ice cover was surprisingly reduced. The IP25 maxima are similar to those determined for the late Holocene. (5) Both, dinoflagellate and IP25/PIP25 data indicate that also during the Late Pliocene Warming Event at least occasionally sea ice must have occurred. (6) This low-resolution pilot study motivates to carry out further detailed high-resolution sea-ice biomarker research on ODP/IODP material in order to prove or disprove these preliminary interpretations. References Belt, S.T., Massé, G., Rowland, S.J., Poulin, M., Michel, C., LeBlanc, B., 2007. A novel chemical fossil of palaeo sea ice: IP25. Organic Geochemistry 38, 16-27. Müller, J., Wagner, A., Fahl, K., Stein, R., Prange, M., Lohmann, G., 2011. Towards quantitative sea ice reconstructions in the northern North Atlantic: A combined biomarker and numerical modelling approach. Earth Planetary Science Letters 306, 137-148.

An improved independent component analysis model for 3D chromatogram separation and its solution by multi-areas genetic algorithm

PubMed Central

2014-01-01

Background The 3D chromatogram generated by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) has been researched widely in the field of herbal medicine, grape wine, agriculture, petroleum and so on. Currently, most of the methods used for separating a 3D chromatogram need to know the compounds' number in advance, which could be impossible especially when the compounds are complex or white noise exist. New method which extracts compounds from 3D chromatogram directly is needed. Methods In this paper, a new separation model named parallel Independent Component Analysis constrained by Reference Curve (pICARC) was proposed to transform the separation problem to a multi-parameter optimization issue. It was not necessary to know the number of compounds in the optimization. In order to find all the solutions, an algorithm named multi-areas Genetic Algorithm (mGA) was proposed, where multiple areas of candidate solutions were constructed according to the fitness and distances among the chromosomes. Results Simulations and experiments on a real life HPLC-DAD data set were used to demonstrate our method and its effectiveness. Through simulations, it can be seen that our method can separate 3D chromatogram to chromatogram peaks and spectra successfully even when they severely overlapped. It is also shown by the experiments that our method is effective to solve real HPLC-DAD data set. Conclusions Our method can separate 3D chromatogram successfully without knowing the compounds' number in advance, which is fast and effective. PMID:25474487

Characterization of in vivo-acquired resistance to macrolides of Mycoplasma gallisepticum strains isolated from poultry

PubMed Central

2011-01-01

The macrolide class of antibiotics, including tylosin and tilmicosin, is widely used in the veterinary field for prophylaxis and treatment of mycoplasmosis. In vitro susceptibility testing of 50 strains of M. gallisepticum isolated in Israel during the period 1997-2010 revealed that acquired resistance to tylosin as well as to tilmicosin was present in 50% of them. Moreover, 72% (13/18) of the strains isolated from clinical samples since 2006 showed acquired resistance to enrofloxacin, tylosin and tilmicosin. Molecular typing of the field isolates, performed by gene-target sequencing (GTS), detected 13 molecular types (I-XIII). Type II was the predominant type prior to 2006 whereas type X, first detected in 2008, is currently prevalent. All ten type X strains were resistant to both fluoroquinolones and macrolides, suggesting selective pressure leading to clonal dissemination of resistance. However, this was not a unique event since resistant strains with other GTS molecular types were also found. Concurrently, the molecular basis for macrolide resistance in M. gallisepticum was identified. Our results revealed a clear-cut correlation between single point mutations A2058G or A2059G in domain V of the gene encoding 23S rRNA (rrnA, MGA_01) and acquired macrolide resistance in M. gallisepticum. Indeed, all isolates with MIC ≥ 0.63 μg/mL to tylosin and with MIC ≥ 1.25 μg/mL to tilmicosin possess one of these mutations, suggesting an essential role in decreased susceptibility of M. gallisepticum to 16-membered macrolides. PMID:21810258

Generation and Surface Localization of Intact M Protein in Streptococcus pyogenes Are Dependent on sagA

PubMed Central

Biswas, Indranil; Germon, Pierre; McDade, Kathleen; Scott, June R.

2001-01-01

The M protein is an important surface-located virulence factor of Streptococcus pyogenes, the group A streptococcus (GAS). Expression of M protein is primarily controlled by Mga, a transcriptional activator protein. A recent report suggested that the sag locus, which includes nine genes necessary and sufficient for production of streptolysin S, another GAS virulence factor, is also needed for transcription of emm, encoding the M protein (Z. Li, D. D. Sledjeski, B. Kreikemeyer, A. Podbielski, and M. D. Boyle, J. Bacteriol. 181:6019–6027, 1999). To investigate this in more detail, we constructed an insertion-deletion mutation in sagA, the first gene in the sag locus, in the M6 strain JRS4. The resulting strain, JRS470, produced no detectable streptolysin S and showed a drastic reduction in cell surface-associated M protein, as measured by cell aggregation and Western blot analysis. However, transcription of the emm gene was unaffected by the sagA mutation. Detailed analysis with monoclonal antibodies and an antipeptide antibody showed that the M protein in the sagA mutant strain was truncated so that it lacks the C-repeat region and the C-terminal domain required for anchoring it to the cell surface. This truncated M protein was largely found, as expected, in the culture supernatant. Lack of surface-located M protein made the sagA mutant strain susceptible to phagocytosis. Thus, although sagA does not affect transcription of the M6 protein gene, it is needed for the surface localization of this important virulence factor. PMID:11598078

Multilocus analysis of extracellular putative virulence proteins made by group A Streptococcus: population genetics, human serologic response, and gene transcription.

PubMed

Reid, S D; Green, N M; Buss, J K; Lei, B; Musser, J M

2001-06-19

Species of pathogenic microbes are composed of an array of evolutionarily distinct chromosomal genotypes characterized by diversity in gene content and sequence (allelic variation). The occurrence of substantial genetic diversity has hindered progress in developing a comprehensive understanding of the molecular basis of virulence and new therapeutics such as vaccines. To provide new information that bears on these issues, 11 genes encoding extracellular proteins in the human bacterial pathogen group A Streptococcus identified by analysis of four genomes were studied. Eight of the 11 genes encode proteins with a LPXTG(L) motif that covalently links Gram-positive virulence factors to the bacterial cell surface. Sequence analysis of the 11 genes in 37 geographically and phylogenetically diverse group A Streptococcus strains cultured from patients with different infection types found that recent horizontal gene transfer has contributed substantially to chromosomal diversity. Regions of the inferred proteins likely to interact with the host were identified by molecular population genetic analysis, and Western immunoblot analysis with sera from infected patients confirmed that they were antigenic. Real-time reverse transcriptase-PCR (TaqMan) assays found that transcription of six of the 11 genes was substantially up-regulated in the stationary phase. In addition, transcription of many genes was influenced by the covR and mga trans-acting gene regulatory loci. Multilocus investigation of putative virulence genes by the integrated approach described herein provides an important strategy to aid microbial pathogenesis research and rapidly identify new targets for therapeutics research.

Expression of recombinant green fluorescent protein in Bacillus methanolicus.

PubMed

Nilasari, Dewi; Dover, Nir; Rech, Sabine; Komives, Claire

2012-01-01

Microbial biocatalysts are used in a wide range of industries to produce large scale quantities of proteins, amino acids, and commodity chemicals. While the majority of these processes use glucose or other low-cost sugars as the substrate, Bacillus methanolicus is one example of a biocatalyst that has shown sustained growth on methanol as a carbon source at elevated temperature (50-53°C optimum) resulting in reduced feed and utility costs. Specifically, the complete chemical process enabled by this approach takes methane from natural gas, and following a low-cost conversion to methanol, can be used for the production of high value products. In this study, production of recombinant green fluorescent protein (GFPuv) by B. methanolicus is explored. A plasmid was constructed that incorporates the methanol dehydrogenase (mdh) promoter of B. methanolicus MGA3 together with the GFPuv gene. The plasmid, pNW33N, was shown to be effective for expression in other Bacillus strains, although not previously in B. methanolicus. A published electroporation protocol for transformation of B. methanolicus was modified to result in expression of GFP using plasmid pNW33N-mdh-GFPuv (pNmG). Transformation was confirmed by both agarose gel electrophoresis and by observation of green fluorescence under UV light exposure. The mass yield of cells and protein were measured in shake flask experiments. The optimum concentration of methanol for protein production was found to be at 200 mM. Higher concentrations than 200 mM resulted in slightly higher biomass production but lower amounts of recombinant protein. Copyright © 2012 American Institute of Chemical Engineers (AIChE).

Application of RNAMlet to surface defect identification of steels

NASA Astrophysics Data System (ADS)

Xu, Ke; Xu, Yang; Zhou, Peng; Wang, Lei

2018-06-01

As three main production lines of steels, continuous casting slabs, hot rolled steel plates and cold rolled steel strips have different surface appearances and are produced at different speeds of their production lines. Therefore, the algorithms for the surface defect identifications of the three steel products have different requirements for real-time and anti-interference. The existing algorithms cannot be adaptively applied to surface defect identification of the three steel products. A new method of adaptive multi-scale geometric analysis named RNAMlet was proposed. The idea of RNAMlet came from the non-symmetry anti-packing pattern representation model (NAM). The image is decomposed into a set of rectangular blocks asymmetrically according to gray value changes of image pixels. Then two-dimensional Haar wavelet transform is applied to all blocks. If the image background is complex, the number of blocks is large, and more details of the image are utilized. If the image background is simple, the number of blocks is small, and less computation time is needed. RNAMlet was tested with image samples of the three steel products, and compared with three classical methods of multi-scale geometric analysis, including Contourlet, Shearlet and Tetrolet. For the image samples with complicated backgrounds, such as continuous casting slabs and hot rolled steel plates, the defect identification rate obtained by RNAMlet was 1% higher than other three methods. For the image samples with simple backgrounds, such as cold rolled steel strips, the computation time of RNAMlet was one-tenth of the other three MGA methods, while the defect identification rates obtained by RNAMlet were higher than the other three methods.

Findings from the 2011 EBRI/MGA Consumer Engagement in Health Care Survey.

PubMed

Fronstin, Paul

2011-12-01

SEVENTH ANNUAL SURVEY: This Issue Brief presents findings from the 2011 EBRI/MGA Consumer Engagement in Health Care Survey. This study is based on an online survey of 4,703 privately insured adults ages 21-64 to provide nationally representative data regarding the growth of consumer-driven health plans (CDHPs) and high-deductible health plans (HDHPs), and the impact of these plans and consumer engagement more generally on the behavior and attitudes of adults with private health insurance coverage. Findings from this survey are compared with EBRI's findings from earlier surveys. ENROLLMENT CONTINUES TO GROW: The survey finds continued growth in consumer-driven health plans: In 2011, 7 percent of the population was enrolled in a CDHP, up from 5 percent in 2010. Enrollment in HDHPs increased from 14 percent in 2010 to 16 percent in 2011. The 7 percent of the population with a CDHP represents 8.4 million adults ages 21-64 with private insurance, while the 16 percent with a HDHP represents 19.3 million people. Among the 19.3 million individuals with an HDHP, 38 percent (or 7.3 million) reported that they were eligible for a health savings ccount (HSA) but did not have such an account. Overall, 15.8 million adults ages 21-64 with private insurance, representing 13.1 percent of that market, were either in a CDHP or were in an HDHP that was eligible for an HSA but had not opened the account. When their children are counted, about 21 million individuals with private insurance, representing about 12 percent of the market, were either in a CDHP or an HSA-eligible plan. MORE COST-CONSCIOUS BEHAVIOR: Individuals in CDHPs were more likely than those with traditional coverage to exhibit a number of cost-conscious behaviors. They were more likely to say that they had checked whether their plan would cover care; asked for a generic drug instead of a brand name; talked to their doctor about treatment options and costs; talked to their doctor about prescription drug options and costs; developed a budget to manage health care expenses; checked a price of service before getting care; and used an online cost-tracking tool. CDHP ENROLLEES MORE ENGAGED IN WELLNESS PROGRAMS: CDHP enrollees were more likely than traditional plan enrollees to report that they had the opportunity to fill out a health risk assessment, and they were also more likely to report that they had access to a health promotion program. CDHP enrollees were also more likely to report that they had been offered a cash incentive or reward to participate in a wellness program when a program was offered. HDHP enrollees were less likely to report having the opportunity to fill out a health risk assessment and to have access to a health promotion program. FINANCIAL INCENTIVES MATTER: When it comes to participating in a wellness program, CDHP enrollees were more likely than traditional plan enrollees to take advantage of the health risk assessment but not the health promotion program. Among those participating, the reasons they gave were that they were offered incentive prizes and reduced premiums. Among those not participating, the reasons they gave were that they could make changes on their own; they lacked time; and they were already healthy. Financial incentives were more a factor for CDHP enrollees than for traditional plan enrollees when it came to participating in wellness programs. CONSUMER USE OF TECHNOLOGY: A significant portion of the population reported using a smartphone, and 1 in 5 reported using a tablet. Among them, about one-quarter reported using an app for health-related purposes. Among those not using an app, nearly one-half were interested in using one.

Late-summer sea ice segmentation with multi-polarisation SAR features in C and X band

NASA Astrophysics Data System (ADS)

Fors, Ane S.; Brekke, Camilla; Doulgeris, Anthony P.; Eltoft, Torbjørn; Renner, Angelika H. H.; Gerland, Sebastian

2016-02-01

In this study, we investigate the potential of sea ice segmentation by C- and X-band multi-polarisation synthetic aperture radar (SAR) features during late summer. Five high-resolution satellite SAR scenes were recorded in the Fram Strait covering iceberg-fast first-year and old sea ice during a week with air temperatures varying around 0 °C. Sea ice thickness, surface roughness and aerial photographs were collected during a helicopter flight at the site. Six polarimetric SAR features were extracted for each of the scenes. The ability of the individual SAR features to discriminate between sea ice types and their temporal consistency were examined. All SAR features were found to add value to sea ice type discrimination. Relative kurtosis, geometric brightness, cross-polarisation ratio and co-polarisation correlation angle were found to be temporally consistent in the investigated period, while co-polarisation ratio and co-polarisation correlation magnitude were found to be temporally inconsistent. An automatic feature-based segmentation algorithm was tested both for a full SAR feature set and for a reduced SAR feature set limited to temporally consistent features. In C band, the algorithm produced a good late-summer sea ice segmentation, separating the scenes into segments that could be associated with different sea ice types in the next step. The X-band performance was slightly poorer. Excluding temporally inconsistent SAR features improved the segmentation in one of the X-band scenes.

Modelling of Sea Ice Thermodynamics and Biogeochemistry during the N-ICE2015 Expedition in the Arctic Ocean

NASA Astrophysics Data System (ADS)

Meyer, A.; Duarte, P.; Mork Olsen, L.; Kauko, H.; Assmy, P.; Rösel, A.; Itkin, P.; Hudson, S. R.; Granskog, M. A.; Gerland, S.; Sundfjord, A.; Steen, H.; Jeffery, N.; Hunke, E. C.; Elliott, S.; Turner, A. K.

2016-12-01

Changes in the sea ice regime of the Arctic Ocean over the last decades from a thick perennial multiyear ice to a first year ice have been well documented. These changes in the sea ice regime will affect feedback mechanisms between the sea ice, atmosphere and ocean. Here we evaluate the performance of the Los Alamos Sea Ice Model (CICE), a state of the art sea ice model, to predict sea ice physical and biogeochemical properties at time scales of a few weeks. We also identify the most problematic prognostic variables and what is necessary to improve their forecast. The availability of a complete data set of forcing collected during the Norwegian Young sea Ice (N-ICE-2015) expedition north of Svalbard opens the possibility to properly test CICE. Oceanographic, atmospheric, sea ice, snow, and biological data were collected above, on, and below the ice using R/V Lance as the base for the ice camps that were drifting south towards the Fram Strait. Over six months, four different drifts took place, from the Nansen Basin, through the marginal ice zone, to the open ocean. Obtained results from the model show a good performance regarding ice thickness, salinity and temperature. Nutrients and sea ice algae are however not modelled as accurately. We hypothesize that improvements in biogeochemical modeling may be achieved by complementing brine drainage with a diffusion parameterization and biogeochemical modeling with the introduction of an explicit formulation to forecast chlorophyll and regulate photosynthetic efficiency.

Helicopter- and ship-based measurements of mesoscale ocean color and thermal features in the marginal ice zone

NASA Astrophysics Data System (ADS)

Tanis, Fred J.; Manley, Thomas O.; Mitchell, Brian G.

1990-09-01

Eddies along the Polar Front/Marginal Ice Zone (MIZ) in Fram Strait are thought to make important contributions to nutrient flux and stimulation of primary productivity. During the Coordinated Eastern Arctic Regional Experiment (CEAREX) helicopter-based measurements of upwelling radiance were made in four visible spectral bands and in the thermal IR across mesoscale features associated with the MIZ. These structures were mapped by flying a grid pattern over the ocean surface to define eddy boundaries. Subsequently, the area was also sampled vertically with CTD and spectral radiometer profilers. Data obtained from a single structure were integrated to construct a three dimensional picture of physical and optical properties. Volume modeling of temperature, salinity, and density fields obtained from CTD survey define the subsurface eddy structure and are in good agreement with infrared derived characteristics. Maximum temperature in the core was found to be four degrees higher than the surrounding water. Volume modeling further indicates that a subsurface layer of Arctic Intermediate Water is intrinsically associated with the surface expression of the eddy. The ratio of upwelling radiances, L(44l)/L(565), was found to be correlated to surface chlorophyll, particulate absorption coefficient, and in water determinations of L using the optical profiling system. The remote sensing reflectance ratio along with the IR sea surface temperature were found to be useful to detect the surface expression of the eddy and to indicate near surface biological and physical processes.

Ice Fog and Light Snow Measurements Using a High-Resolution Camera System

NASA Astrophysics Data System (ADS)

Kuhn, Thomas; Gultepe, Ismail

2016-09-01

Ice fog, diamond dust, and light snow usually form over extremely cold weather conditions, and they affect both visibility and Earth's radiative energy budget. Prediction of these hydrometeors using models is difficult because of limited knowledge of the microphysical properties at the small size ranges due to measurement issues. These phenomena need to be better represented in forecast and climate models; therefore, in addition to remote sensing accurate measurements using ground-based instrumentation are required. An imaging instrument, aimed at measuring ice fog and light snow particles, has been built and is presented here. The ice crystal imaging (ICI) probe samples ice particles into a vertical, tapered inlet with an inlet flow rate of 11 L min-1. A laser beam across the vertical air flow containing the ice crystals allows for their detection by a photodetector collecting the scattered light. Detected particles are then imaged with high optical resolution. An illuminating LED flash and image capturing are triggered by the photodetector. In this work, ICI measurements collected during the fog remote sensing and modeling (FRAM) project, which took place during Winter of 2010-2011 in Yellowknife, NWT, Canada, are summarized and challenges related to measuring small ice particles are described. The majority of ice particles during the 2-month-long campaign had sizes between 300 and 800 μm. During ice fog events the size distribution measured had a lower mode diameter of 300 μm compared to the overall campaign average with mode at 500 μm.

Measurement of Arctic sea-ice thickness by submarine 5 years after SCICEX

NASA Astrophysics Data System (ADS)

Hughes, Nicholas E.; Wadhams, Peter

In April 2004 the Royal Navy submarine HMS Tireless became the first UK submarine to conduct environmental monitoring in the Arctic Ocean since 1996. As the last US SCICEX (Scientific Ice Expeditions) cruise was in 2000, this has been the only opportunity for a civilian scientist to carry out measurement of ice draft and oceanography over a wide area of the Arctic. This paper presents preliminary results and compares them with similar investigations in the 1970s-90s. The route of Tireless covered a large area of the European sector of the Arctic from 5° E to 62° W. Transects were carried out from the marginal ice zone in Fram Strait up to the North Pole and along the 85° N parallel north of Greenland. As part of work for the European Commission IRIS project, image intensity from the advanced synthetic aperture radar instrument on the European Space Agency's Envisat satellite has been compared with ice draft from the submarine. The raw data were found to be highly variable, so a moving average was applied, producing a correlation of 0.79. Tireless carried a full oceanographic sensor suite and expendable probes for investigation into changes in the Arctic Ocean. The results from these show further erosion of the Arctic cold halocline layer by advancing Atlantic Water compared to previous climatologies and fieldwork expeditions. Preliminary ice-draft data from 85° N show deeper ice keels than those encountered by a submarine on the same route in 1987.

Evaluation of Arctic Sea Ice Thickness Simulated by Arctic Ocean Model Intercomparison Project Models

NASA Technical Reports Server (NTRS)

Johnson, Mark; Proshuntinsky, Andrew; Aksenov, Yevgeny; Nguyen, An T.; Lindsay, Ron; Haas, Christian; Zhang, Jinlun; Diansky, Nikolay; Kwok, Ron; Maslowski, Wieslaw;

Multiple grid arrangement improves ligand docking with unknown binding sites: Application to the inverse docking problem.

PubMed

Ban, Tomohiro; Ohue, Masahito; Akiyama, Yutaka

2018-04-01

The identification of comprehensive drug-target interactions is important in drug discovery. Although numerous computational methods have been developed over the years, a gold standard technique has not been established. Computational ligand docking and structure-based drug design allow researchers to predict the binding affinity between a compound and a target protein, and thus, they are often used to virtually screen compound libraries. In addition, docking techniques have also been applied to the virtual screening of target proteins (inverse docking) to predict target proteins of a drug candidate. Nevertheless, a more accurate docking method is currently required. In this study, we proposed a method in which a predicted ligand-binding site is covered by multiple grids, termed multiple grid arrangement. Notably, multiple grid arrangement facilitates the conformational search for a grid-based ligand docking software and can be applied to the state-of-the-art commercial docking software Glide (Schrödinger, LLC). We validated the proposed method by re-docking with the Astex diverse benchmark dataset and blind binding site situations, which improved the correct prediction rate of the top scoring docking pose from 27.1% to 34.1%; however, only a slight improvement in target prediction accuracy was observed with inverse docking scenarios. These findings highlight the limitations and challenges of current scoring functions and the need for more accurate docking methods. The proposed multiple grid arrangement method was implemented in Glide by modifying a cross-docking script for Glide, xglide.py. The script of our method is freely available online at http://www.bi.cs.titech.ac.jp/mga_glide/. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

Regulators of pseudohyphal differentiation in Saccharomyces cerevisiae identified through multicopy suppressor analysis in ammonium permease mutant strains.

PubMed Central

Lorenz, M C; Heitman, J

1998-01-01

Nitrogen-starved diploid cells of the yeast Saccharomyces cerevisiae differentiate into a filamentous, pseudohyphal growth form. Recognition of nitrogen starvation is mediated, at least in part, by the ammonium permease Mep2p and the Galpha subunit Gpa2p. Genetic activation of the pheromone-responsive MAP kinase cascade, which is also required for filamentous growth, only weakly suppresses the filamentation defect of Deltamep2/Deltamep2 and Deltagpa2/Deltagpa2 strain. Surprisingly, deletion of Mep1p, an ammonium permease not previously thought to regulate differentiation, significantly enhances the potency of MAP kinase activation, such that the STE11-4 allele induces filamentation to near wild-type levels in Deltamep1/Deltamep1 Deltamep2/Deltamep2 and Deltamep1/Deltamep1 Deltagpa2/Deltagpa2 strains. To identify additional regulatory components, we isolated high-copy suppressors of the filamentation defect of the Deltamep1/Deltamep1 Deltamep2/Deltamep2 mutant. Multicopy expression of TEC1, PHD1, PHD2 (MSS10/MSN1/FUP4), MSN5, CDC6, MSS11, MGA1, SKN7, DOT6, HMS1, HMS2, or MEP2 each restored filamentation in a Deltamep1/Deltamep1 Deltamep2/Deltamep2 strain. Overexpression of SRK1 (SSD1), URE2, DAL80, MEP1, or MEP3 suppressed only the growth defect of the Deltamep1/Deltamep1 Deltamep2/Deltamep2 mutant strain. Characterization of these genes through deletion analysis and epistasis underscores the complexity of this developmental pathway and suggests that stress conditions other than nitrogen deprivation may also promote filamentous growth. PMID:9832522

Noncentrosymmetric rare-earth copper gallium chalcogenides RE3CuGaCh7 (RE=La-Nd; Ch=S, Se): An unexpected combination

NASA Astrophysics Data System (ADS)

Iyer, Abishek K.; Rudyk, Brent W.; Lin, Xinsong; Singh, Harpreet; Sharma, Arzoo Z.; Wiebe, Christopher R.; Mar, Arthur

2015-09-01

The quaternary rare-earth chalcogenides RE3CuGaS7 and RE3CuGaSe7 (RE=La-Nd) have been prepared by reactions of the elements at 1050 °C and 900 °C, respectively. They crystallize in the noncentrosymmetric La3CuSiS7-type structure (hexagonal, space group P63, Z=2) in which the a-parameter is largely controlled by the RE component (a=10.0-10.3 Å for the sulfides and 10.3-10.6 Å for the selenides) whereas the c-parameter is essentially fixed by the choice of Ga and chalcogen atoms within tetrahedral units (c=6.1 Å for the sulfides and 6.4 Å for the selenides). They extend the series RE3MGaCh7, previously known for divalent metal atoms (M=Mn-Ni), differing in that the Cu atoms in RE3CuGaCh7 occupy trigonal planar sites instead of octahedral sites. Among quaternary chalcogenides RE3MM‧Ch7, the combination of monovalent (M=Cu) and trivalent (M‧=Ga) metals is unusual because it appears to violate the condition of charge balance satisfied by most La3CuSiS7-type compounds. The possibility of divalent Cu atoms was ruled out by bond valence sum analysis, magnetic measurements, and X-ray photoelectron spectroscopy. The electron deficiency in RE3CuGaCh7 is accommodated through S-based holes at the top of the valence band, as shown by band structure calculations on La3CuGaS7. An optical band gap of about 2.0 eV was found for La3CuGaSe7.

A glioma-derived analog to platelet-derived growth factor: demonstration of receptor competing activity and immunological crossreactivity.

PubMed Central

Nistér, M; Heldin, C H; Wasteson, A; Westermark, B

1984-01-01

A human clonal glioma cell line, U-343 MGa Cl 2, cultured under serum-free conditions, was found to release a factor that competed with 125I-labeled platelet-derived growth factor (125I-PDGF) for binding to human foreskin fibroblasts. The concentration of competing activity in conditioned medium was equal to 20-30 ng of PDGF per ml. The PDGF receptor competing activity had an elution position on Sephadex G-200 close to that of tracer PDGF. The same fractions in the chromatogram also contained growth-promoting activity and material active in a PDGF radioimmunoassay. Incubation of partially purified, 125I-labeled glioma factor with fibroblasts, or rabbit anti-PDGF serum, led to the selective binding of a component with an estimated Mr of 31,000, as shown by NaDodSO4/gel electrophoresis under nonreducing conditions. After reduction this component migrated as a Mr 18,000 protein. Thus, the behavior in NaDodSO4/gel electrophoresis was similar to that of PDGF. Furthermore, incubation of partially purified glioma factor with immobilized PDGF antibodies markedly decreased the amount of PDGF receptor competing activity remaining in the supernatant. These results suggest that the factor produced by glioma cells has structural, immunological, and functional resemblance to PDGF. We previously reported that a human osteosarcoma cell line produces a PDGF-like molecule with growth-promoting activity. Taken together with the recent finding that PDGF is homologous to the transforming gene product of simian sarcoma virus, our present data give additional support for the idea that an autocrine activation of the PDGF receptor may be operational in the growth of human tumors of mesenchymal or glial origin. Images PMID:6322178

Testing of the BipiColombo Antenna Pointing Mechanism

NASA Astrophysics Data System (ADS)

Campo, Pablo; Barrio, Aingeru; Martin, Fernando

2015-09-01

BepiColombo is an ESA mission to Mercury, its planetary orbiter (MPO) has two antenna pointing mechanism, High gain antenna (HGA) pointing mechanism steers and points a large reflector which is integrated at system level by TAS-I Rome. Medium gain antenna (MGA) APM points a 1.5 m boom with a horn antenna. Both radiating elements are exposed to sun fluxes as high as 10 solar constants without protections.A previous paper [1] described the design and development process to solve the challenges of performing in harsh environment.. Current paper is focused on the testing process of the qualification units. Testing performance of antenna pointing mechanism in its specific environmental conditions has required special set-up and techniques. The process has provided valuable feedback on the design and the testing methods which have been included in the PFM design and tests.Some of the technologies and components were developed on dedicated items priort to EQM, but once integrated, test behaviour had relevant differences.Some of the major concerns for the APM testing are:- Create during the thermal vacuum testing the qualification temperature map with gradients along the APM. From of 200oC to 70oC.- Test in that conditions the radio frequency and pointing performances adding also high RF power to check the power handling and self-heating of the rotary joint.- Test in life up to 12000 equivalent APM revolutions, that is 14.3 million motor revolutions in different thermal conditions.- Measure low thermal distortion of the mechanical chain, being at the same time insulated from external environment and interfaces (55 arcsec pointing error)- Perform deployment of large items guaranteeing during the process low humidity, below 5% to protect dry lubrication- Verify stability with representative inertia of large boom or reflector 20 Kgm2.

The methylotrophic Bacillus methanolicus MGA3 possesses two distinct fructose 1,6-bisphosphate aldolases.

PubMed

Stolzenberger, Jessica; Lindner, Steffen N; Wendisch, Volker F

2013-08-01

The thermotolerant Gram-positive methylotroph Bacillus methanolicus is able to grow with methanol, glucose or mannitol as a sole carbon and energy source. Fructose 1,6-bisphosphate aldolase (FBA), a key enzyme of glycolysis and gluconeogenesis, is encoded in the genome of B. methanolicus by two putative fba genes, the chromosomally located fba(C) and fba(P) on the naturally occurring plasmid pBM19. Their amino acid sequences share 75 % identity and suggest a classification as class II aldolases. Both enzymes were purified from recombinant Escherichia coli and were found to be active as homotetramers. Both enzymes were activated by either manganese or cobalt ions, and inhibited by ADP, ATP and EDTA. The kinetic parameters allowed us to distinguish the chromosomally encoded FBA(C) from the plasmid encoded FBA(P), since FBA(C) showed higher affinity towards fructose 1,6-bisphosphate (Km of 0.16±0.01 mM as compared to 2±0.08 mM) as well as higher glycolytic catalytic efficiency (31.3 as compared to 0.8 s(-1) mM(-1)) than FBA(P). However, FBA(P) exhibited a higher catalytic efficiency in gluconeogenesis (50.4 as compared to 1.4 s(-1) mM(-1) with dihydroxyacetone phosphate and 4 as compared to 0.4 s(-1) mM(-1) with glyceraldehyde 3-phosphate as limiting substrate). The aldolase-negative Corynebacterium glutamicum mutant Δfda could be complemented with both FBA genes from B. methanolicus. Based on the kinetic data, we propose that FBA(C) acts as major aldolase in glycolysis, whereas FBA(P) acts as major aldolase in gluconeogenesis in B. methanolicus.

Calibration of sea ice dynamic parameters in an ocean-sea ice model using an ensemble Kalman filter

NASA Astrophysics Data System (ADS)

Massonnet, F.; Goosse, H.; Fichefet, T.; Counillon, F.

2014-07-01

The choice of parameter values is crucial in the course of sea ice model development, since parameters largely affect the modeled mean sea ice state. Manual tuning of parameters will soon become impractical, as sea ice models will likely include more parameters to calibrate, leading to an exponential increase of the number of possible combinations to test. Objective and automatic methods for parameter calibration are thus progressively called on to replace the traditional heuristic, "trial-and-error" recipes. Here a method for calibration of parameters based on the ensemble Kalman filter is implemented, tested and validated in the ocean-sea ice model NEMO-LIM3. Three dynamic parameters are calibrated: the ice strength parameter P*, the ocean-sea ice drag parameter Cw, and the atmosphere-sea ice drag parameter Ca. In twin, perfect-model experiments, the default parameter values are retrieved within 1 year of simulation. Using 2007-2012 real sea ice drift data, the calibration of the ice strength parameter P* and the oceanic drag parameter Cw improves clearly the Arctic sea ice drift properties. It is found that the estimation of the atmospheric drag Ca is not necessary if P* and Cw are already estimated. The large reduction in the sea ice speed bias with calibrated parameters comes with a slight overestimation of the winter sea ice areal export through Fram Strait and a slight improvement in the sea ice thickness distribution. Overall, the estimation of parameters with the ensemble Kalman filter represents an encouraging alternative to manual tuning for ocean-sea ice models.

Cascading off the West Greenland Shelf: A numerical perspective

NASA Astrophysics Data System (ADS)

Marson, Juliana M.; Myers, Paul G.; Hu, Xianmin; Petrie, Brian; Azetsu-Scott, Kumiko; Lee, Craig M.

2017-07-01

Cascading of dense water from the shelf to deeper layers of the adjacent ocean basin has been observed in several locations around the world. The West Greenland Shelf (WGS), however, is a region where this process has never been documented. In this study, we use a numerical model with a 1/4° resolution to determine (i) if cascading could happen from the WGS; (ii) where and when it could take place; (iii) the forcings that induce or halt this process; and (iv) the path of the dense plume. Results show cascading happening off the WGS at Davis Strait. Dense waters form there due to brine rejection and slide down the slope during spring. Once the dense plume leaves the shelf, it gradually mixes with waters of similar density and moves northward into Baffin Bay. Our simulation showed events happening between 2003-2006 and during 2014; but no plume was observed in the simulation between 2007 and 2013. We suggest that the reason why cascading was halted in this period is related to: the increased freshwater transport from the Arctic Ocean through Fram Strait; the additional sea ice melting in the region; and the reduced presence of Irminger Water at Davis Strait during fall/early winter. Although observations at Davis Strait show that our simulation usually overestimates the seasonal range of temperature and salinity, they agree with the overall variability captured by the model. This suggests that cascades have the potential to develop on the WGS, albeit less dense than the ones estimated by the simulation.

Subsurface Xenon Migration by Atmospheric Pumping Using an Implicit Non-Iterative Algorithm for a Locally 1D Dual-Porosity Model

NASA Astrophysics Data System (ADS)

Annewandter, R.; Kalinowksi, M. B.

2009-04-01

An underground nuclear explosion injects radionuclids in the surrounding host rock creating an initial radionuclid distribution. In the case of fractured permeable media, cyclical changes in atmospheric pressure can draw gaseous species upwards to the surface, establishing a ratcheting pump effect. The resulting advective transport is orders of magnitude more significant than transport by molecular diffusion. In the 1990s the US Department of Energy funded the socalled Non-Proliferation Experiment conducted by the Lawrence Livermore National Laboratory to investigate this barometric pumping effect for verifying compliance with respect to the Comprehensive Nuclear Test Ban Treaty. A chemical explosive of approximately 1 kt TNT-equivalent has been detonated in a cavity located 390 m deep in the Rainier Mesa (Nevada Test Site) in which two tracer gases were emplaced. Within this experiment SF6 was first detected in soil gas samples taken near fault zones after 50 days and 3He after 325 days. For this paper a locally one-dimensional dual-porosity model for flow along the fracture and within the permeable matrix was used after Nilson and Lie (1990). Seepage of gases and diffusion of tracers between fracture and matrix are accounted. The advective flow along the fracture and within the matrix block is based on the FRAM filtering remedy and methodology of Chapman. The resulting system of equations is solved by an implicit non-iterative algorithm. Results on time of arrival and subsurface concentration levels for the CTBT-relevant xenons will be presented.

Hydrographic changes in the Lincoln Sea in the Arctic Ocean with focus on an upper ocean freshwater anomaly between 2007 and 2010

NASA Astrophysics Data System (ADS)

de Steur, L.; Steele, M.; Hansen, E.; Morison, J.; Polyakov, I.; Olsen, S. M.; Melling, H.; McLaughlin, F. A.; Kwok, R.; Smethie, W. M.; Schlosser, P.

2013-09-01

Hydrographic data from the Arctic Ocean show that freshwater content in the Lincoln Sea, north of Greenland, increased significantly from 2007 to 2010, slightly lagging changes in the eastern and central Arctic. The anomaly was primarily caused by a decrease in the upper ocean salinity. In 2011 upper ocean salinities in the Lincoln Sea returned to values similar to those prior to 2007. Throughout 2008-2010, the freshest surface waters in the western Lincoln Sea show water mass properties similar to fresh Canada Basin waters north of the Canadian Arctic Archipelago. In the northeastern Lincoln Sea fresh surface waters showed a strong link with those observed in the Makarov Basin near the North Pole. The freshening in the Lincoln Sea was associated with a return of a subsurface Pacific Water temperature signal although this was not as strong as observed in the early 1990s. Comparison of repeat stations from the 2000s with the data from the 1990s at 65°W showed an increase of the Atlantic temperature maximum which was associated with the arrival of warmer Atlantic water from the Eurasian Basin. Satellite-derived dynamic ocean topography of winter 2009 showed a ridge extending parallel to the Canadian Archipelago shelf as far as the Lincoln Sea, causing a strong flow toward Nares Strait and likely Fram Strait. The total volume of anomalous freshwater observed in the Lincoln Sea and exported by 2011 was close to 1100±250km3, approximately 13% of the total estimated FW increase in the Arctic in 2008.

Arctic Deep Water Ferromanganese-Oxide Deposits Reflect the Unique Characteristics of the Arctic Ocean

NASA Astrophysics Data System (ADS)

Hein, James R.; Konstantinova, Natalia; Mikesell, Mariah; Mizell, Kira; Fitzsimmons, Jessica N.; Lam, Phoebe J.; Jensen, Laramie T.; Xiang, Yang; Gartman, Amy; Cherkashov, Georgy; Hutchinson, Deborah R.; Till, Claire P.

2017-11-01

Little is known about marine mineral deposits in the Arctic Ocean, an ocean dominated by continental shelf and basins semi-closed to deep-water circulation. Here, we present data for ferromanganese crusts and nodules collected from the Amerasia Arctic Ocean in 2008, 2009, and 2012 (HLY0805, HLY0905, and HLY1202). We determined mineral and chemical compositions of the crusts and nodules and the onset of their formation. Water column samples from the GEOTRACES program were analyzed for dissolved and particulate scandium concentrations, an element uniquely enriched in these deposits. The Arctic crusts and nodules are characterized by unique mineral and chemical compositions with atypically high growth rates, detrital contents, Fe/Mn ratios, and low Si/Al ratios, compared to deposits found elsewhere. High detritus reflects erosion of submarine outcrops and North America and Siberia cratons, transport by rivers and glaciers to the sea, and distribution by sea ice, brines, and currents. Uniquely high Fe/Mn ratios are attributed to expansive continental shelves, where diagenetic cycling releases Fe to bottom waters, and density flows transport shelf bottom water to the open Arctic Ocean. Low Mn contents reflect the lack of a mid-water oxygen minimum zone that would act as a reservoir for dissolved Mn. The potential host phases and sources for elements with uniquely high contents are discussed with an emphasis on scandium. Scandium sorption onto Fe oxyhydroxides and Sc-rich detritus account for atypically high scandium contents. The opening of Fram Strait in the Miocene and ventilation of the deep basins initiated Fe-Mn crust growth ˜15 Myr ago.

A Meteoric Water Budget for the Arctic Ocean

NASA Astrophysics Data System (ADS)

Alkire, Matthew B.; Morison, James; Schweiger, Axel; Zhang, Jinlun; Steele, Michael; Peralta-Ferriz, Cecilia; Dickinson, Suzanne

2017-12-01

A budget of meteoric water (MW = river runoff, net precipitation minus evaporation, and glacial meltwater) over four regions of the Arctic Ocean is constructed using a simple box model, regional precipitation-evaporation estimates from reanalysis data sets, and estimates of import and export fluxes derived from the literature with a focus on the 2003-2008 period. The budget indicates an approximate/slightly positive balance between MW imports and exports (i.e., no change in storage); thus, the observed total freshwater increase observed during this time period likely resulted primarily from changes in non-MW freshwater components (i.e., increases in sea ice melt or Pacific water and/or a decrease in ice export). Further, our analysis indicates that the MW increase observed in the Canada Basin resulted from a spatial redistribution of MW over the Arctic Ocean. Mean residence times for MW were estimated for the Western Arctic (5-7 years), Eastern Arctic (3-4 years), and Lincoln Sea (1-2 years). The MW content over the Siberian shelves was estimated (˜14,000 km3) based on a residence time of 3.5 years. The MW content over the entire Arctic Ocean was estimated to be ≥44,000 km3. The MW export through Fram Strait consisted mostly of water from the Eastern Arctic (3,237 ± 1,370 km3 yr-1) whereas the export through the Canadian Archipelago was nearly equally derived from both the Western Arctic (1,182 ± 534 km3 yr-1) and Lincoln Sea (972 ± 391 km3 yr-1).

Plio-Pleistocene evolution of water mass exchange and erosional input at the Atlantic-Arctic gateway

NASA Astrophysics Data System (ADS)

Teschner, Claudia; Frank, Martin; Haley, Brian A.; Knies, Jochen

2016-05-01

Water mass exchange between the Arctic Ocean and the Norwegian-Greenland Seas has played an important role for the Atlantic thermohaline circulation and Northern Hemisphere climate. We reconstruct past water mass mixing and erosional inputs from the radiogenic isotope compositions of neodymium (Nd), lead (Pb), and strontium (Sr) at Ocean Drilling Program site 911 (leg 151) from 906 m water depth on Yermak Plateau in the Fram Strait over the past 5.2 Myr. The isotopic compositions of past bottom waters were extracted from authigenic oxyhydroxide coatings of the bulk sediments. Neodymium isotope signatures obtained from surface sediments agree well with present-day deepwater ɛNd signature of -11.0 ± 0.2. Prior to 2.7 Ma the Nd and Pb isotope compositions of the bottom waters only show small variations indicative of a consistent influence of Atlantic waters. Since the major intensification of the Northern Hemisphere Glaciation at 2.7 Ma the seawater Nd isotope composition has varied more pronouncedly due to changes in weathering inputs related to the waxing and waning of the ice sheets on Svalbard, the Barents Sea, and the Eurasian shelf, due to changes in water mass exchange and due to the increasing supply of ice-rafted debris (IRD) originating from the Arctic Ocean. The seawater Pb isotope record also exhibits a higher short-term variability after 2.7 Ma, but there is also a trend toward more radiogenic values, which reflects a combination of changes in input sources and enhanced incongruent weathering inputs of Pb released from freshly eroded old continental rocks.

Regional- and local-scale variations in benthic megafaunal composition at the Arctic deep-sea observatory HAUSGARTEN

NASA Astrophysics Data System (ADS)

Taylor, J.; Krumpen, T.; Soltwedel, T.; Gutt, J.; Bergmann, M.

2016-02-01

The Long-Term Ecological Research (LTER) observatory HAUSGARTEN, in the eastern Fram Strait, provides us the valuable ability to study the composition of benthic megafaunal communities through the analysis of seafloor photographs. This, in combination with extensive sampling campaigns, which have yielded a unique data set on faunal, bacterial, biogeochemical and geological properties, as well as on hydrography and sedimentation patterns, allows us to address the question of why variations in megafaunal community structure and species distribution exist within regional (60-110 km) and local (<4 km) scales. Here, we present first results from the latitudinal HAUSGARTEN gradient, consisting of three different stations (N3, HG-IV, S3) between 78°30‧N and 79°45‧N (2351-2788 m depth), obtained via the analysis of images acquired by a towed camera (OFOS-Ocean Floor Observation System) in 2011. We assess variability in megafaunal densities, species composition and diversity as well as biotic and biogenic habitat features, which may cause the patterns observed. While there were significant regional-scale differences in megafaunal composition and densities between the stations (N3=26.74±0.63; HG-IV=11.21±0.25; S3=18.34±0.39 individuals m-2), significant local differences were only found at HG-IV. Regional-scale variations may be due to the significant differences in ice coverage at each station as well as the different quantities of protein available, whereas local-scale differences at HG-IV may be a result of variation in bottom topography or factors not yet identified.

Arctic deep-water ferromanganese-oxide deposits reflect the unique characteristics of the Arctic Ocean

USGS Publications Warehouse

Hein, James; Konstantinova, Natalia; Mikesell, Mariah; Mizell, Kira; Fitzsimmons, Jessica N.; Lam, Phoebe; Jensen, Laramie T.; Xiang, Yang; Gartman, Amy; Cherkashov, Georgy; Hutchinson, Deborah; Till, Claire P.

2017-01-01

Little is known about marine mineral deposits in the Arctic Ocean, an ocean dominated by continental shelf and basins semi-closed to deep-water circulation. Here, we present data for ferromanganese crusts and nodules collected from the Amerasia Arctic Ocean in 2008, 2009, and 2012 (HLY0805, HLY0905, HLY1202). We determined mineral and chemical compositions of the crusts and nodules and the onset of their formation. Water column samples from the GEOTRACES program were analyzed for dissolved and particulate scandium concentrations, an element uniquely enriched in these deposits.The Arctic crusts and nodules are characterized by unique mineral and chemical compositions with atypically high growth rates, detrital contents, Fe/Mn ratios, and low Si/Al ratios, compared to deposits found elsewhere. High detritus reflects erosion of submarine outcrops and North America and Siberia cratons, transport by rivers and glaciers to the sea, and distribution by sea ice, brines, and currents. Uniquely high Fe/Mn ratios are attributed to expansive continental shelves, where diagenetic cycling releases Fe to bottom waters, and density flows transport shelf bottom water to the open Arctic Ocean. Low Mn contents reflect the lack of a mid-water oxygen minimum zone that would act as a reservoir for dissolved Mn. The potential host phases and sources for elements with uniquely high contents are discussed with an emphasis on scandium. Scandium sorption onto Fe oxyhydroxides and Sc-rich detritus account for atypically high scandium contents. The opening of Fram Strait in the Miocene and ventilation of the deep basins initiated Fe-Mn crust growth ∼15 Myr ago.

On the Flow of Atlantic Water Towards the Arctic Ocean; a Synergy Between Altimetry and Hydrography.

NASA Astrophysics Data System (ADS)

Chafik, L.; Nilsson, J.; Skagseth, O.; Lundberg, P.

2015-12-01

The Arctic climate is strongly influenced by the inflow of warm Atlantic water conveyed by the Norwegian Atlantic Slope Current (NwASC); the main heat conveyor into the Arctic Ocean. Based on sea surface height (SSH) data from altimetry, we develop a dynamical measure of the NwASC transport to diagnose its spatio-temporal variability. This supports a dynamical division of the NwASC into two flow regimes; the Svinøy Branch (SvB) in the Norwegian Sea, and the Fram Strait Branch (FSB) west of Spitsbergen. The SvB transport is well correlated with the SSH and atmospheric variability within the Nordic Seas, factors that also affect the inflow to the Barents Sea. In contrast, the FSB is regulated by regional atmospheric patterns around Svalbard and northern Barents Sea. We further relate anomalous flow events to temperature fluctuations of Atlantic water. A warm anomaly is found to propagate northwards, with a tendency to amplify enroute, after events of strong flow in the Norwegian Sea. A roughly 12-months delayed temperature signal is identified in the FSB. This suggests that hydrographic anomalies both upstream from the North Atlantic, and locally generated in the Norwegian Sea, are important for the oceanic heat and salt transport that eventually enters into the Arctic. We believe that the combination of the flow from altimetry and temperature fluctuations in the Nordic Seas can be used to qualitatively predict warm anomalies towards the Arctic Ocean, which could be a valuable addition to the forecast skill of the statistical Arctic sea-ice models.

Production of Liquid Synthetic Fuels from Carbon, Water and Nuclear Power on Ships and at Shore Bases for Military and Potential Commercial Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Locke Bogart, S.; Schultz, Ken; Brown, Lloyd

2006-07-01

It is demonstrable that synthetic fuels (jet/diesel/gasoline {approx_equal} (CH{sub 2}){sub n}) can be produced from carbon, water, and nuclear energy. What remains to be shown is that all system processes are scalable, integrable, and economical. Sources of carbon include but are not limited to CO{sub 2} from the atmosphere or seawater, CO{sub 2} from fossil-fired power plants, and elemental carbon from coal or biomass. For mobile defense (Navy) applications, the ubiquitous atmosphere is our chosen carbon source. For larger-scale sites such as Naval Advance Bases, the atmosphere may still be the choice should other sources not be readily available. However, at many locations suitable for defense and, potentially, commercial syn-fuel production, far higher concentrations of carbon may be available. The rationale for this study was manifold: fuel system security from terrorism and possible oil embargoes; rising demand and, eventually, peaking supply of conventional petroleum; and escalating costs and prices of fuels. For these reasons, the initial parts of the study were directed at Syn-fuel production for mobile Naval platforms and shore sites such as Rokkasho, Japan (as an exemplar). Nuclear reactors would provide the energy for H{sub 2} from water-splitting, Membrane Gas Absorption (MGA) would extract CO{sub 2} from the atmosphere, the Reverse Water-Gas Reaction (RWGR) would convert the CO{sub 2} to CO, and the resultant H{sub 2} and CO feeds would be converted to (CH{sub 2})n by the Fischer-Tropsch reaction. Many of these processes exist at commercial scale. Some, particularly MGA and RWGR, have been demonstrated at the bench-scale, requiring up-scaling. Likewise, the demonstration of an integrated system at some scale is yet to be done. For ship-based production, it has been shown that the system should be viable and, under reasonable assumptions, both scalable and economical for defense fuels. For the assumptions in the study, fuel cost estimates range from {approx}more » $2.55 to $$4.75 per gallon with a nominal cost of {approx} $$3.65 per gallon. For large installations and advanced nuclear power and hydrogen production systems (high temperature reactors and thermo-chemical hydrogen production), then fuel production might be produced at near-commercial fuel prices. For the H2-MHR and plausible assumptions and estimates of CO{sub 2} extraction and fuel synthesis capital and operating costs, such fuels might have nominal and low production costs ranging from {approx} $2.40 to $$1.70 per gallon, respectively, for a Public Sector Fixed Charge Rate of 5%. Next, it was shown that for CO{sub 2} provided from a fossil-fired power plant, a CO{sub 2} 'disposal' fee of $$30/tonne and a Fixed Charge Rate of 10%, then syn-fuel might be produced at {approx} $3.00 and $2.45 (nominal cost values) and $1.90 and $$1.85 (low cost values) per gallon by LWRs and H2-MHRs, respectively. Last, it was shown that nuclear-produced H{sub 2} and O{sub 2} could convert coal to liquid fuels at very low cost. For a Fixed Charge Rate of 10% and nominal plant costs, fuel costs ranged from {approx} $$1.60 (LWR) per gallon to {approx} $$1.30 (H2-MHR) for an assumed CO{sub 2} avoidance credit of $$30/Tonne. Our studies have shown that the addition of nuclear-produced hydrogen and oxygen to the coal syn-fuel process can greatly reduce CO{sub 2} production and, for modest CO{sub 2} credit, can further reduce the cost of the syn-fuel. Capturing CO{sub 2} from stack gas or even the air will further reduce the amount of CO{sub 2} that must be dealt with. This last case is independent of the price of fossil fuels and liquid fuel production costs and prices will have been capped. Of possibly even greater importance, the carbon fuel cycle will have been closed, thus minimizing or eliminating concerns with Global Climate Change. (authors)« less

Increasing frequency and duration of Arctic winter warming events

NASA Astrophysics Data System (ADS)

Graham, R. M.; Cohen, L.; Petty, A.; Boisvert, L.; Rinke, A.; Hudson, S. R.; Nicolaus, M.; Granskog, M. A.

2017-12-01

Record low Arctic sea ice extents were observed during the last three winter seasons (March). During each of these winters, near-surface air temperatures close to 0°C were observed, in situ, over sea ice in the central Arctic. Recent media reports and scientific studies suggest that such winter warming events were unprecedented for the Arctic. Here we use in situ winter (December-March) temperature observations, such as those from Soviet North Pole drifting stations and ocean buoys, to determine how common Arctic winter warming events are. The earliest record we find of a winter warming event was in March 1896, where a temperature of -3.7˚C was observed at 84˚N during the Fram expedition. Observations of winter warming events exist over most of the Arctic Basin. Despite a limited observational network, temperatures exceeding -5°C were measured in situ during more than 30% of winters from 1954 to 2010, by either North Pole drifting stations or ocean buoys. Correlation coefficients between the atmospheric reanalysis, ERA-Interim, and these in-situ temperature records are shown to be on the order of 0.90. This suggests that ERA-Interim is a suitable tool for studying Arctic winter warming events. Using the ERA-Interim record (1979-2016), we show that the North Pole (NP) region typically experiences 10 warming events (T2m > -10°C) per winter, compared with only five in the Pacific Central Arctic (PCA). We find a positive trend in the overall duration of winter warming events for both the NP region (4.25 days/decade) and PCA (1.16 days/decade), due to an increased number of events of longer duration.

Pathways of Atlantic Waters in the Nordic seas: locally eddy-permitting ocean simulation in a global setup

NASA Astrophysics Data System (ADS)

Wekerle, C.; Wang, Q.; Danilov, S.; Jung, T.; Schourup-Kristensen, V.

2016-02-01

Atlantic Water (AW) passes through the Nordic Seas and enters the Arctic Ocean through the shallow Barents Sea and the deep Fram Strait. Since the 1990's, observations indicate a series of anomalously warm pulses of Atlantic Water that entered the Arctic Ocean. In fact, poleward oceanic heat transport may even increase in the future, which might have implications for the heat uptake in the Arctic Ocean as well as for the sea ice cover. The ability of models to faithfully simulate the pathway of the AW and accompanying dynamics is thus of high climate relevance. In this study, we explore the potential of a global multi-resolution sea ice-ocean model with a locally eddy-permitting resolution (around 4.5 km) in the Nordic seas region and Arctic Ocean in improving the representation of Atlantic Water inflow, and more broadly, the dynamics of the circulation in the Northern North Atlantic and Arctic. The simulation covers the time period 1969-2009. We find that locally increased resolution improves the localization and thickness of the Atlantic Water layer in the Nordic seas, compared with a 20 km resolution reference simulation. In particular, the inflow of Atlantic Waters through the Greenland Scotland Ridge and the narrow branches of the Norwegian Atlantic Current can be realistically represented. Lateral spreading due to simulated eddies essentially reduces the bias in the surface temperature. In addition, a qualitatively good agreement of the simulated eddy kinetic energy field with observations can be achieved. This study indicates that a substantial improvement in representing local ocean dynamics can be reached through the local refinement, which requires a rather moderate computational effort. The successful model assessment allows us to further investigate the variability and mechanisms behind Atlantic Water transport into the Arctic Ocean.

Tracking the Mediterranean Abyss

NASA Astrophysics Data System (ADS)

Aracri, S.; Schroeder, K.; Chiggiato, J.; Bryden, H. L.; McDonagh, E.; Josey, S. A.; Hello, Y.; Borghini, M.

2016-02-01

The Mediterranean Sea is well known to be a miniature ocean with small enough timescales to allow the observation of main oceanographic events, e.g. deep water formation and overturning circulation, in a human life time. This renders the Mediterranean Sea the perfect observatory to study and forecast the behaviour of the world ocean. Considering the coherence between NAO (North Atlantic Oscillation), AMO (Atlantic Multidecadal Oscillation) and Mediterranean oscillation and bearing in mind that the Mediterranean outflow at Gibraltar constitutes a constant source of intermediate, warm and saline water, it has been suggested that "the system composed of the North Atlantic, the Mediterranean Sea/Gibraltar Strait and the Arctic Sea/Fram Strait might work as a unique oceanographic entity, with the physical processes within the straits determining the exchange of the fresh and salty waters between the marginal seas and the open ocean".In the light of the present knowledge the Mediterranean might, then, be considered as a key oceanographic observatory site. The deep sea is still challenging to monitor, especially given the latest years lack of fundings and ships availability. Therefore optimizing the existing methods and instrumentation has become a priority. This work is focused on the North-Western Mediterranean basin, where deep water formation events often occur in the Gulf of Lion as well as deep convection in the neighbour Ligurian Sea. A different application of submarine robots - Mermaids- designed to observe underwater seismic waves aiming to improve ocean tomography is presented. In order to improve our knowledge of the North-Western Mediterranean abyssal circulation we track Mermaids extracting their velocity, correcting it and comparing it with the historically estimated values and with the geostrophic velocity extracted from a 40 years long hydrographic datasets.

Acoustic detections of summer and winter whales at Arctic gateways in the Atlantic and Pacific Oceans

NASA Astrophysics Data System (ADS)

Stafford, K.; Laidre, K. L.; Moore, S. E.

2016-02-01

Changes in sea ice phenology have been profound in regions north of arctic gateways, where the seasonal open-water period has increased by 1.5-3 months over the past 30 years. This has resulted in changes to the Arctic ecosystem, including increased primary productivity, changing food web structure, and opening of new habitat. In the "new normal" Arctic, ice obligate species such as ice seals and polar bears may fare poorly under reduced sea ice while sub-arctic "summer" whales (fin and humpback) are poised to inhabit new seasonal ice-free habitats in the Arctic. We examined the spatial and seasonal occurrence of summer and "winter" (bowhead) whales from September through December by deploying hydrophones in three Arctic gateways: Bering, Davis and Fram Straits. Acoustic occurrence of the three species was compared with decadal-scale changes in seasonal sea ice. In all three Straits, fin whale acoustic detections extended from summer to late autumn. Humpback whales showed the same pattern in Bering and Davis Straits, singing into November and December, respectively. Bowhead whale detections generally began after the departure of the summer whales and continued through the winter. In all three straits, summer whales occurred in seasons and regions that used to be ice-covered. This is likely due to both increased available habitat from sea ice reductions and post-whaling population recoveries. At present, in the straits examined here, there is spatial, but not temporal, overlap between summer and winter whales. In a future with further seasonal sea ice reductions, however, increased competition for resources between sub-Arctic and Arctic species may arise to the detriment of winter whales.

Open-source sea ice drift algorithm for Sentinel-1 SAR imagery using a combination of feature-tracking and pattern-matching

NASA Astrophysics Data System (ADS)

Muckenhuber, Stefan; Sandven, Stein

2017-04-01

An open-source sea ice drift algorithm for Sentinel-1 SAR imagery is introduced based on the combination of feature-tracking and pattern-matching. A computational efficient feature-tracking algorithm produces an initial drift estimate and limits the search area for the pattern-matching, that provides small to medium scale drift adjustments and normalised cross correlation values as quality measure. The algorithm is designed to utilise the respective advantages of the two approaches and allows drift calculation at user defined locations. The pre-processing of the Sentinel-1 data has been optimised to retrieve a feature distribution that depends less on SAR backscatter peak values. A recommended parameter set for the algorithm has been found using a representative image pair over Fram Strait and 350 manually derived drift vectors as validation. Applying the algorithm with this parameter setting, sea ice drift retrieval with a vector spacing of 8 km on Sentinel-1 images covering 400 km x 400 km, takes less than 3.5 minutes on a standard 2.7 GHz processor with 8 GB memory. For validation, buoy GPS data, collected in 2015 between 15th January and 22nd April and covering an area from 81° N to 83.5° N and 12° E to 27° E, have been compared to calculated drift results from 261 corresponding Sentinel-1 image pairs. We found a logarithmic distribution of the error with a peak at 300 m. All software requirements necessary for applying the presented sea ice drift algorithm are open-source to ensure free implementation and easy distribution.

Small-incision 4-point scleral suture fixation of a foldable hydrophilic acrylic intraocular lens in the absence of capsule support.

PubMed

Terveen, Daniel C; Fram, Nicole R; Ayres, Brandon; Berdahl, John P

2016-02-01

To determine the visual outcomes and complications of a new small-incision technique for 4-point fixation of a hydrophilic acrylic posterior chamber intraocular lens (IOL) in the absence of capsule support. Three North American tertiary referral centers and a private practice. Retrospective case series. Secondary IOL placement was performed from 2011 to 2014. Indications for surgery, clinical results, and complications were analyzed. Primary outcomes included postoperative corrected distance visual acuity (CDVA) and surgical complications. Charts of 35 patients (37 eyes) were reviewed. The mean age at surgery was 56 years, and the mean follow-up was 6 months (range 3 to 24 months). Clinical indications included a dislocated IOL (30%), ocular trauma (19%), crystalline lens subluxation (19%), uveitis-glaucoma-hyphema syndrome (5%), post-complicated cataract (8%), congenital cataract (8%), and decompensated cornea (3%). The mean CDVA improved from 20/80 preoperatively to 20/40 postoperatively (P < .01). Thirty-six eyes (97%) had an improved or unchanged CDVA, and 1 eye (3%) had a reduced CDVA because of worsening glaucoma. Postoperative complications included ocular hypertension (24%), iritis (5%), wound leakage (3%), transient corneal edema (3%), glaucoma requiring a tube shunt (3%), and IOL dislocation (3%). Small-incision 4-point scleral fixation of the Akreos AO60 hydrophilic acrylic IOL in the absence of capsule support appears to be a safe and effective technique for secondary IOL placement. Drs. Berdahl and Ayres are consultants to Bausch & Lomb, Inc. Drs. Ayres and Fram have received speaker fees from Bausch & Lomb. Dr. Terveen does not have a financial or proprietary interest in any material or methods mentioned. Copyright © 2016 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.

Ice fog and light snow measurements using a high resolution camera system

NASA Astrophysics Data System (ADS)

Kuhn, Thomas; Gultepe, Ismail

2016-04-01

In this presentation, measurements collected by the ice crystal imaging (ICI) probe employed during FRAM (Fog Remote Sensing and Modeling) project for the Winter of 2010-2011 in Yellowknife, NWT, Canada are analysed to study small ice crystal impact on aviation operations. Ice fog, diamond dust, and light snow form during cold weather conditions and they affect aviation operations through visibility and deposition over the surfaces. In addition, these events influence the local heat budget through radiative cooling. Prediction of these hydrometeors using models is difficult because of limited knowledge of the microphysical properties at the small size ranges. These phenomena need to be better represented in forecast and climate models and this can only be done using accurate measurements from ground-based instrumentation. Imaging of ice particles' properties can complement other in-situ measurements being collected routinely. The newly developed ICI probe, aimed at measuring ice fog and light snow particles, is presented here. The ICI probe samples ice particles through a vertical inlet, where a laser beam and photodetector detect ice crystals contained in the flow. The detected particles are then imaged with high optical resolution between 10 to 1000 micron size range. An illuminating LED flash and image capturing for measurements are triggered by the photodetector. The results suggested that the majority of ice particles during the two-month long campaign were small with sizes between 300 μm and 800 μm. During ice fog events, the size distribution measured had a lower mode diameter of 300 μm compared to the overall campaign average with mode at 500 μm. In this presentation, challenges and issues related to small ice crystals are described and their importance for aviation operations and climate change are discussed.

Modelling the impacts of a dipole-like climatic state over the Arctic

NASA Astrophysics Data System (ADS)

Pasha Karami, Mehdi; de Vernal, Anne; Hu, Xianmin; Myers, Paul G.

2015-04-01

The Arctic dipole anomaly (ADA) features a pattern with opposite sea-level pressure anomalies over the Canadian Archipelago and the Barents Sea. Changes in the predominance of Arctic atmospheric circulation modes and the shift towards a dipole mode in the past decade played a role in the summer sea ice loss and sea ice-freshwater export from the Arctic to the North Atlantic. Reconstruction of sea ice cover variations during Holocene also suggests opposite anomalies in the Barents Sea versus either the western Arctic or the Fram Strait area similar to the ADA pattern. It is vital to study such physical processes that cause dramatic changes in the Arctic sea ice recalling the link between the ADA and the current climate change. Here we focus on the question of how a persistent ADA-like state affects the Arctic sea ice distribution and its outflow to the Atlantic Ocean. For this purpose, an eddy-permitting regional configuration of the NEMO coupled ocean/sea-ice model is used. The regional domain covers the Arctic Ocean and the Northern-Hemisphere Atlantic, with a horizontal resolution of 1/4 degree at the equator (ANHA4). For the present-day simulations, boundary conditions are obtained by taking the average over the years with a positive ADA and those with a negative ADA. In the Holocene scenario, global climate model data are used to force our regional model. To exclude the role of Bering Strait and the heat flux from the Pacific Ocean, we repeat the experiments with a closed Bering Strait since a nearly closed Bering configuration was possible for the Early Holocene. The model results are compared with the paleoclimate data from Arctic and subarctic seas.

The smallest Gliders in the Ocean- Temperature Recordings from Pteropods using SIMS

NASA Astrophysics Data System (ADS)

Keul, N.; Orland, I. J.; Valley, J. W.; Kozdon, R.; deMenocal, P. B.

2016-02-01

During the last few decades, the development, refinement and application of geochemical methods have lead to the establishment of new proxies to estimate global change. The oxygen isotope composition of carbonate shells formed by marine organisms is a widely used proxy for past ocean temperatures. Secondary ion mass spectrometry (SIMS) is a high spatial-resolution in situ technique, allowing oxygen isotope measurements on very small samples (down to 3 μm spot size). Pteropods are pelagic mollusks, producing shells made out of aragonite, which is more soluble than calcite in seawater, making them one of the first responders to climate change. They calcify closely at the aragonite- seawater equilibrium and are therefor ideal candidates to reconstruct temperatures based on their d18O. The oxygen isotopic composition of pteropod shells from sediment traps, net tows and a culture study from the Fram Strait will be presented. Specimens travelled in the Westspitsbergen Current prior to sinking into the sediment. During this transport specimens continue to grow, sequentially adding aragonite to the shell. We show that when using traditional (whole shell) δ18O measurements, the isotopic signatures of whole shells reflect the latitudinal gradient. Combining this with SIMS-derived δ18O measurements on individual shell parts, however, reveals that this is only half the story: Comparing δ18O of earlier (produced further South) and later shell parts (produced further North) shows that pteropods calcify in sequentially shallower water depths, overriding the latitudinal South to North temperature gradient. Combining traditional and novel δ18O measurements can potentially not only allow the reconstruction of temperature/ global warming, but also to assess ecological key parameters, such as habitat depth, at the same time.

Ikaite crystals in melting sea ice - implications for pCO2 and pH levels in Arctic surface waters

NASA Astrophysics Data System (ADS)

Rysgaard, S.; Glud, R. N.; Lennert, K.; Cooper, M.; Halden, N.; Leakey, R. J. G.; Hawthorne, F. C.; Barber, D.

2012-08-01

A major issue of Arctic marine science is to understand whether the Arctic Ocean is, or will be, a source or sink for air-sea CO2 exchange. This has been complicated by the recent discoveries of ikaite (a polymorph of CaCO3·6H2O) in Arctic and Antarctic sea ice, which indicate that multiple chemical transformations occur in sea ice with a possible effect on CO2 and pH conditions in surface waters. Here, we report on biogeochemical conditions, microscopic examinations and x-ray diffraction analysis of single crystals from a melting 1.7 km2 (0.5-1 m thick) drifting ice floe in the Fram Strait during summer. Our findings show that ikaite crystals are present throughout the sea ice but with larger crystals appearing in the upper ice layers. Ikaite crystals placed at elevated temperatures disintegrated into smaller crystallites and dissolved. During our field campaign in late June, melt reduced the ice floe thickness by 0.2 m per week and resulted in an estimated 3.8 ppm decrease of pCO2 in the ocean surface mixed layer. This corresponds to an air-sea CO2 uptake of 10.6 mmol m-2 sea ice d-1 or to 3.3 ton km-2 ice floe week-1. This is markedly higher than the estimated primary production within the ice floe of 0.3-1.3 mmol m-2 sea ice d-1. Finally, the presence of ikaite in sea ice and the dissolution of the mineral during melting of the sea ice and mixing of the melt water into the surface oceanic mixed layer accounted for half of the estimated pCO2 uptake.

Nonlinear dead water resistance at subcritical speed

NASA Astrophysics Data System (ADS)

Grue, John

2015-08-01

The dead water resistance F 1 = /1 2 C d w ρ S U 2 (ρ fluid density, U ship speed, S wetted body surface, Cdw resistance coefficient) on a ship moving at subcritical speed along the upper layer of a two-layer fluid is calculated by a strongly nonlinear method assuming potential flow in each layer. The ship dimensions correspond to those of the Polar ship Fram. The ship draught, b0, is varied in the range 0.25h0-0.9h0 (h0 the upper layer depth). The calculations show that Cdw/(b0/h0)2 depends on the Froude number only, in the range close to critical speed, Fr = U/c0 ˜ 0.875-1.125 (c0 the linear internal long wave speed), irrespective of the ship draught. The function Cdw/(b0/h0)2 attains a maximum at subcritical Froude number depending on the draught. Maximum Cdw/(b0/h0)2 becomes 0.15 for Fr = 0.76, b0/h0 = 0.9, and 0.16 for Fr = 0.74, b0/h0 = 1, where the latter extrapolated value of the dead water resistance coefficient is about 60 times higher than the frictional drag coefficient and relevant for the historical dead water observations. The nonlinear Cdw significantly exceeds linear theory (Fr < 0.85). The ship generated waves have a wave height comparable to the upper layer depth. Calculations of three-dimensional wave patterns at critical speed compare well to available laboratory experiments. Upstream solitary waves are generated in a wave tank of finite width, when the layer depths differ, causing an oscillation of the force. In a wide ocean, a very wide wave system develops at critical speed. The force approaches a constant value for increasing time.

Arctic lead detection using a waveform unmixing algorithm from CryoSat-2 data

NASA Astrophysics Data System (ADS)

Lee, S.; Im, J.

2016-12-01

Arctic areas consist of ice floes, leads, and polynyas. While leads and polynyas account for small parts in the Arctic Ocean, they play a key role in exchanging heat flux, moisture, and momentum between the atmosphere and ocean in wintertime because of their huge temperature difference In this study, a linear waveform unmixing approach was proposed to detect lead fraction. CryoSat-2 waveforms for pure leads, sea ice, and ocean were used as end-members based on visual interpretation of MODIS images coincident with CryoSat-2 data. The unmixing model produced lead, sea ice, and ocean abundances and a threshold (> 0.7) was applied to make a binary classification between lead and sea ice. The unmixing model produced better results than the existing models in the literature, which are based on simple thresholding approaches. The results were also comparable with our previous research using machine learning based models (i.e., decision trees and random forest). A monthly lead fraction was calculated, dividing the number of detected leads by the total number of measurements. The lead fraction around Beaufort Sea and Fram strait was high due to the anti-cyclonic rotation of Beaufort Gyre and the outflows of sea ice to the Atlantic. The lead fraction maps produced in this study were matched well with monthly lead fraction maps in the literature. The areas with thin sea ice identified from our previous research correspond to the high lead fraction areas in the present study. Furthermore, sea ice roughness from ASCAT scatterometer was compared to a lead fraction map to see the relationship between surface roughness and lead distribution.

Seismic and Thermal Structure of the Arctic Lithosphere, From Waveform Tomography and Thermodynamic Modelling

NASA Astrophysics Data System (ADS)

Lebedev, S.; Schaeffer, A. J.; Fullea, J.; Pease, V.

2015-12-01

Thermal structure of the lithosphere is reflected in the values of seismic velocities within it. Our new tomographic models of the crust and upper mantle of the Arctic are constrained by an unprecedentedly large global waveform dataset and provide substantially improved resolution, compared to previous models. The new tomography reveals lateral variations in the temperature and thickness of the lithosphere and defines deep boundaries between tectonic blocks with different lithospheric properties and age. The shape and evolution of the geotherm beneath a tectonic unit depends on both crustal and mantle-lithosphere structure beneath it: the lithospheric thickness and its changes with time (these determine the supply of heat from the deep Earth), the crustal thickness and heat production (the supply of heat from within the crust), and the thickness and thermal conductivity of the sedimentary cover (the insulation). Detailed thermal structure of the basins can be modelled by combining seismic velocities from tomography with data on the crustal structure and heat production, in the framework of computational petrological modelling. The most prominent lateral contrasts across the Arctic are between the cold, thick lithospheres of the cratons (in North America, Greenland and Eurasia) and the warmer, non-cratonic blocks. The lithosphere of the Canada Basin is cold and thick, similar to old oceanic lithosphere elsewhere around the world; its thermal structure offers evidence on its lithospheric age and formation mechanism. At 150-250 km depth, the central Arctic region shows a moderate low-velocity anomaly, cooler than that beneath Iceland and N Atlantic. An extension of N Atlantic low-velocity anomaly into the Arctic through the Fram Strait may indicate an influx of N Atlantic asthenosphere under the currently opening Eurasia Basin.

Arctic multiyear ice classification and summer ice cover using passive microwave satellite data

NASA Astrophysics Data System (ADS)

Comiso, J. C.

1990-08-01

The ability to classify and monitor Arctic multiyear sea ice cover using multispectral passive microwave data is studied. Sea ice concentration maps during several summer minima have been analyzed to obtain estimates of ice surviving the summer. The results are compared with multiyear ice concentrations derived from data the following winter, using an algorithm that assumes a certain emissivity for multiyear ice. The multiyear ice cover inferred from the winter data is approximately 25 to 40% less than the summer ice cover minimum, suggesting that even during winter when the emissivity of sea ice is most stable, passive microwave data may account for only a fraction of the total multiyear ice cover. The difference of about 2×106 km2 is considerably more than estimates of advection through Fram Strait during the intervening period. It appears that as in the Antarctic, some multiyear ice floes in the Arctic, especially those near the summer marginal ice zone, have first-year ice or intermediate signatures in the subsequent winter. A likely mechanism for this is the intrusion of seawater into the snow-ice interface, which often occurs near the marginal ice zone or in areas where snow load is heavy. Spatial variations in melt and melt ponding effects also contribute to the complexity of the microwave emissivity of multiyear ice. Hence the multiyear ice data should be studied in conjunction with the previous summer ice data to obtain a more complete characterization of the state of the Arctic ice cover. The total extent and actual areas of the summertime Arctic pack ice were estimated to be 8.4×106 km2 and 6.2×106 km2, respectively, and exhibit small interannual variability during the years 1979 through 1985, suggesting a relatively stable ice cover.

Alkenone-based reconstructions show four-phase Holocene temperature history for Arctic Svalbard

NASA Astrophysics Data System (ADS)

van der Bilt, W. G. M.; D'Andrea, W. J.; Bakke, J.; Balascio, N.; Werner, J.; Bradley, R. S.

2016-12-01

Situated at the crossroads of global oceanic and atmospheric circulation patterns, the Arctic is a key component of Earth`s climate system. Amplified by sea-ice feedbacks, even modest shifts in regional heat budget drive large climate responses. This is highlighted by the dramatic response of the Arctic to global warming. Assessing the signature of underlying forcings require paleoclimate records, allowing us to expand our knowledge beyond the short instrumental period and contextualize ongoing warming. However, such data are scarce and sparse in the Arctic, limiting our ability to address these issues. We present two quantitative Holocene-length summer temperature reconstructions from the Arctic Svalbard archipelago. Temperature estimates are based on alkenone unsaturation ratios measured on sediment cores from two lakes. Our data reveal a dynamic Holocene temperature history, with reconstructed lake water temperatures spanning a range of 6-8 °C, and characterized by four phases. The Early Holocene was marked by an early ( 10.5 ka cal. BP) onset of insolation-driven Hypsithermal conditions, likely compounded by strengthening oceanic heat transport. This warm interval was interrupted by cooling between 10.5-8.3 ka cal. BP that we attribute to cooling effects from the melting Northern Hemisphere ice sheets. Temperatures declined throughout the Middle Holocene, following a gradual trend that was accentuated by two cooling steps between 7.8-7 ka cal. BP and 4.4-3.5 ka cal. BP. These transitions coincide with a strengthening influence of Arctic water and sea-ice in the adjacent eastern Fram Strait. During the Late Holocene (past 4 ka), temperature change decoupled from the still-declining insolation, and fluctuated around cold mean conditions. This study improves our understanding of Arctic climate dynamics by demonstrating that Holocene Svalbard temperatures were governed by an alternation of forcing mechanism.

Simple anthropometric measures correlate with metabolic risk indicators as strongly as magnetic resonance imaging–measured adipose tissue depots in both HIV-infected and control subjects2

PubMed Central

Scherzer, Rebecca; Shen, Wei; Bacchetti, Peter; Kotler, Donald; Lewis, Cora E; Shlipak, Michael G; Heymsfield, Steven B

2008-01-01

Background Studies in persons without HIV infection have compared percentage body fat (%BF) and waist circumference as markers of risk for the complications of excess adiposity, but only limited study has been conducted in HIV-infected subjects. Objective We compared anthropometric and magnetic resonance imaging (MRI)–based adiposity measures as correlates of metabolic complications of adiposity in HIV-infected and control subjects. Design The study was a cross-sectional analysis of 666 HIV-positive and 242 control subjects in the Fat Redistribution and Metabolic Change in HIV Infection (FRAM) study assessing body mass index (BMI), waist (WC) and hip (HC) circumferences, waist-to-hip ratio (WHR), %BF, and MRI-measured regional adipose tissue. Study outcomes were 3 metabolic risk variables [homeostatic model assessment (HOMA), triglycerides, and HDL cholesterol]. Analyses were stratified by sex and HIV status and adjusted for demographic, lifestyle, and HIV-related factors. Results In HIV-infected and control subjects, univariate associations with HOMA, triglycerides, and HDL were strongest for WC, MRI-measured visceral adipose tissue, and WHR; in all cases, differences in correlation between the strongest measures for each outcome were small (r ≤ 0.07). Multivariate adjustment found no significant difference for optimally fitting models between the use of anthropometric and MRI measures, and the magnitudes of differences were small (adjusted R2 ≤ 0.06). For HOMA and HDL, WC appeared to be the best anthropometric correlate of metabolic complications, whereas, for triglycerides, the best was WHR. Conclusion Relations of simple anthropometric measures with HOMA, triglycerides, and HDL cholesterol are approximately as strong as MRI-measured whole-body adipose tissue depots in both HIV-infected and control subjects. PMID:18541572

Simple anthropometric measures correlate with metabolic risk indicators as strongly as magnetic resonance imaging-measured adipose tissue depots in both HIV-infected and control subjects.

PubMed

Scherzer, Rebecca; Shen, Wei; Bacchetti, Peter; Kotler, Donald; Lewis, Cora E; Shlipak, Michael G; Heymsfield, Steven B; Grunfeld, Carl

2008-06-01

Studies in persons without HIV infection have compared percentage body fat (%BF) and waist circumference as markers of risk for the complications of excess adiposity, but only limited study has been conducted in HIV-infected subjects. We compared anthropometric and magnetic resonance imaging (MRI)-based adiposity measures as correlates of metabolic complications of adiposity in HIV-infected and control subjects. The study was a cross-sectional analysis of 666 HIV-positive and 242 control subjects in the Fat Redistribution and Metabolic Change in HIV Infection (FRAM) study assessing body mass index (BMI), waist (WC) and hip (HC) circumferences, waist-to-hip ratio (WHR), %BF, and MRI-measured regional adipose tissue. Study outcomes were 3 metabolic risk variables [homeostatic model assessment (HOMA), triglycerides, and HDL cholesterol]. Analyses were stratified by sex and HIV status and adjusted for demographic, lifestyle, and HIV-related factors. In HIV-infected and control subjects, univariate associations with HOMA, triglycerides, and HDL were strongest for WC, MRI-measured visceral adipose tissue, and WHR; in all cases, differences in correlation between the strongest measures for each outcome were small (r

The relation between AMOC, gyre circulation, and meridional heat transports in the North Atlantic in model simulations of the last millennium

NASA Astrophysics Data System (ADS)

Jungclaus, J. H.; Moreno-Chamarro, E.; Lohmann, K.; Zanchettin, D.

2016-02-01

While it is clear that the Atlantic Meridional Overturning Circulation (AMOC) is responsible for meridional heat transfer from the South Atlantic and the tropics to the North Atlantic, the majority of the heat transport in the northern North Atlantic and the Nordic seas is carried by the gyre system. However, the detailed mechanisms determining the interaction between and the temporal modulation of the components of the northward heat transport system are not clear. Long-term climate records and model simulations can help to identify important processes and to provide background for the changes that are presently observed. Multi-centennial proxy records from the subpolar North Atlantic and the Nordic Seas indicate, for example, an out-of-phase behavior of sea surface temperature and gyre circulation between the two regions with consequences for regional climate. Paleoceanographic evidence from Fram Strait shows a pronounced modulation of heat transfer to the Arctic by the Atlantic Water layer during the last 2000 years and reconstructions from the Subpolar North Atlantic suggest a role of ocean circulation in the transition between the Medieval Climate Anomaly and the Little Ice Age. Here we explore a small ensemble of last millennium simulations, carried out with the Max Planck Institute Earth System Model, and analyze mechanisms connecting the AMOC and gyre circulation and their relation to external forcing. Our results support the important role of the Subpolar Gyre strength and the related meridional mass and temperature fluxes. We find that the modulation of the northward heat transport into the Nordic Seas and the Arctic has pronounced impact on sea-ice distribution, ocean-atmosphere interaction, and the surface climate in Scandinavia and Western Europe.

The relation between AMOC, gyre circulation, and meridional heat transports in the North Atlantic in model simulations of the last millennium

NASA Astrophysics Data System (ADS)

Jungclaus, Johann; Moreno-Chamarro, Eduardo; Lohmann, Katja

2016-04-01

While it is clear that the Atlantic Meridional Overturning Circulation (AMOC) is responsible for meridional heat transfer from the South Atlantic and the tropics to the North Atlantic, the majority of the heat transport in the northern North Atlantic and the Nordic seas is carried by the gyre system. However, the detailed mechanisms determining the interaction between and the temporal modulation of the components of the northward heat transport system are not clear. Long-term climate records and model simulations can help to identify important processes and to provide background for the changes that are presently observed. Multi-centennial proxy records from the subpolar North Atlantic and the Nordic Seas indicate, for example, an out-of-phase behavior of sea surface temperature and gyre circulation between the two regions with consequences for regional climate. Paleoceanographic evidence from Fram Strait shows a pronounced modulation of heat transfer to the Arctic by the Atlantic Water layer during the last 2000 years and reconstructions from the Subpolar North Atlantic suggest a role of ocean circulation in the transition between the Medieval Climate Anomaly and the Little Ice Age. Here we explore a small ensemble of last millennium simulations, carried out with the Max Planck Institute Earth System Model, and analyze mechanisms connecting the AMOC and gyre circulation and their relation to external forcing. Our results support the important role of the Subpolar Gyre strength and the related meridional mass and temperature fluxes. We find that the modulation of the northward heat transport into the Nordic Seas and the Arctic has pronounced impact on sea-ice distribution, ocean-atmosphere interaction, and the surface climate in Scandinavia and Western Europe.

Cenozoic Motion of Greenland - Overlaps and Seaways

NASA Astrophysics Data System (ADS)

Lawver, L. A.; Norton, I. O.; Gahagan, L.

2014-12-01

Using the seafloor magnetic anomalies found in the Labrador Sea, North Atlantic and Eurasian basin to constrain the Cenozoic motion of Greenland, we have produced a new model for the tectonic evolution of the region. The aeromagnetic data collected by the Naval Research Lab [Brozena et al., 2003] in the Eurasian Basin and Canadian data from the Labrador Sea have been re-evaluated using new gridding algorithms and profile modeling using ModMag (Mendel et al., 2005). As a consequence, we have changed the published correlations, mostly prior to Chron C6 [19.05 Ma]. Presently published seafloor magnetic anomalies from the Labrador Sea assume that seafloor spreading ceased at C13 [33.06 Ma] but such an assumption produces an unacceptable overlap of Kronprins Christian Land of northeast Greenland with Svalbard, up to 140 km of overlap in some models. Our new model does not need any "unacceptable" overlap but does produce a slight amount of Eocene compression on Svalbard as is found on land there. Our model allows for an Early Eocene seaway between Ellesmere Island and northwest Greenland that may have connected the Labrador Sea through Baffin Bay and ultimately to the nascent Eurasian Basin, although its depth or even its essential existence is unknowable. During the Miocene, there is no room for a deepwater seaway in Fram Strait until at least the very end of the Early Miocene and perhaps not until Middle Miocene. Brozena, J. and six others, 2003. New aerogeophysical study of the Eurasia Basin and Lomonosov Ridge: Implications for basin development. Geology 31, 825-828. Mendel, V., M. Munschy and D.Sauter, 2005, MODMAG, a MATLAB program to model marine magnetic anomalies, Comp. Geosci., 31, .589-597

UAV applications for thermodynamic profiling: Emphasis on ice fog research

NASA Astrophysics Data System (ADS)

Gultepe, Ismail; Heymsfield, Andrew J.; Fernando, Harindra J. S.; Hoch, Sebastian W.; Ware, Randolph

2016-04-01

Ice fog occurs often over the Arctic, cold climatic, and mountainous regions for about 30% of time where temperature (T) can go down to -10°C or below. Ice Nucleation (IN) and cooling processes play an important role by the controlling the intensity of ice fog conditions that affect aviation application, transportation, and local climate. Ice fog can also occur at T above -10°C but close to 0°C it occurs due to freezing of supercooled droplets that include an IN. To better document ice fog conditions, observations from the ice fog events of the Indirect and Semi-Direct Aerosol effects on Climate (ISDAC) project, Barrow, Alaska, Fog Remote Sensing And Modeling (FRAM) project Yellowknife, Northwest Territories, and the Mountain Terrain Atmospheric Modeling and Observations (MATERHORN) project, Heber City, Utah, were analyzed.. Measurements difficulties of small ice fog particles at cold temperatures and low-level flying restrictions prevent observations from aircraft within the surface boundary layer. However, unmanned Aerial Vehicles (UAVs) can be operated safely to measure IN number concentration, Relative Humidity with respect to ice (RHi), T, horizontal wind speed (Uh) and direction, and ice crystal spectra less than about 500 micron. Thermodynamic profiling by a Radiometrics Profiling Microwave Radiometer (PMWR) and Vaisala CL51 ceilometer was used to describe ice fog conditions in the vertical and its time development. In this presentation, ice fog characteristics and its thermodynamic environment will be presented using both ground-based and airborne platforms such as a UAV with new sensors. Some examples of measurements from the UAV for future research, and challenges related to both ice fog measurements and visibility parameterization will also be presented.

UAV Applications for Thermodynamic Profiling:Emphasis on Ice Fog Visibility

NASA Astrophysics Data System (ADS)

Gultepe, Ismail; Heymsfield, Andrew; Fernando, Joseph; hoch, sebastian; pardyjack, Eric; Boudala, faisal; Ware, Randolph

2017-04-01

Ice fog often occurs over the Arctic, in cold climates, and near mountainous regions about 30% of time when temperatures (T) drop to -10°C or below. Ice fog affects aviation operations, transportation, and local climate. Ice Nucleation (IN) and radiative cooling play an important role by controlling the intensity of ice fog conditions. Ice fog can also occur at T above -10°C, but close to 0°C it mainly occurs due to freezing of supercooled droplets that contain an IN. To better document ice fog conditions, observations from ice fog events of the Indirect and Semi-Direct Aerosol effects on Climate (ISDAC) project (Barrow, Alaska), Fog Remote Sensing And Modeling (FRAM) project (Yellowknife, Northwest Territories), and the Mountain Terrain Atmospheric Modeling and Observations (MATERHORN) project (Heber City, Utah), were analyzed. Difficulties in measuring small ice fog particles at low temperatures and low-level research aircraft flying restrictions prevent observations from aircraft within the atmospheric boundary layer. However, Unmanned Aerial Vehicles (UAVs) can be operated safely to measure IN number concentration, Relative Humidity with respect to ice (RHi), T, horizontal wind speed (Uh) and direction, visibility, and possibly even measuring ice crystal spectra below about 500 micron, to provide a method for future research of ice fog. In this study, thermodynamic profiling was conducted using a Radiometrics Microwave Radiometer (PMWR) and Vaisala CL51 ceilometer to describe vertical spatial and temporal development of ice fog conditions. Overall, ice fog characteristics and its thermodynamic environment will be presented using both ground-based and airborne platforms such as a UAV with new sensors. Some examples of measurements from the UAV and a DMT GCIP (Droplet Measurement Technologies Ground Cloud Imaging Probe), and challenges related to both ice fog measurements and visibility parameterization will also be presented.

Effectiveness and Sensitivity of the Arctic Observing Network in a Coupled Ocean-Sea Ice State Estimation Framework

NASA Astrophysics Data System (ADS)

Nguyen, A. T.; Heimbach, P.; Garg, V.; Ocana, V.

2016-12-01

Over the last few decades, various agencies have invested heavily in the development and deployment of Arctic ocean and sea ice observing platforms, especially moorings, profilers, gliders, and satellite-based instruments. These observational assets are heterogeneous in terms of variables sampled and spatio-temporal coverage, which calls for a dynamical synthesis framework of the diverse data streams. Here we introduce an adjoint-based Arctic Subpolar gyre sTate estimate (ASTE), a medium resolution model-data synthesis that leverages all the possible observational assets. Through an established formal state and parameter estimation framework, the ASTE framework produces a 2002-present ocean-sea ice state that can be used to address Arctic System science questions. It is dynamically and kinematically consistent with known equations of motion and consistent with observations. Four key aspects of ASTE will be discussed: (1) How well is ASTE constrained by the existing observations; (2) which data most effectively constrain the system, and what impact on the solution does spatial and temporal coverage have; (3) how much information does one set of observation (e.g. Fram Strait heat transport) carry about a remote, but dynamically linked component (e.g. heat content in the Beaufort Gyre); and (4) how can the framework be used to assess the value of hypothetical observations in constraining poorly observed parts of the Arctic Ocean and the implied mechanisms responsible for the changes occurring in the Arctic. We will discuss the suggested geographic distribution of new observations to maximize the impact on improving our understanding of the general circulation, water mass distribution and hydrographic changes in the Arctic.

Linking atmospheric synoptic transport, cloud phase, surface energy fluxes, and sea-ice growth: observations of midwinter SHEBA conditions

NASA Astrophysics Data System (ADS)

Persson, P. Ola G.; Shupe, Matthew D.; Perovich, Don; Solomon, Amy

2017-08-01

Observations from the Surface Heat Budget of the Arctic Ocean (SHEBA) project are used to describe a sequence of events linking midwinter long-range advection of atmospheric heat and moisture into the Arctic Basin, formation of supercooled liquid water clouds, enhancement of net surface energy fluxes through increased downwelling longwave radiation, and reduction in near-surface conductive heat flux loss due to a warming of the surface, thereby leading to a reduction in sea-ice bottom growth. The analyses provide details of two events during Jan. 1-12, 1998, one entering the Arctic through Fram Strait and the other from northeast Siberia; winter statistics extend the results. Both deep, precipitating frontal clouds and post-frontal stratocumulus clouds impact the surface radiation and energy budget. Cloud liquid water, occurring preferentially in stratocumulus clouds extending into the base of the inversion, provides the strongest impact on surface radiation and hence modulates the surface forcing, as found previously. The observations suggest a minimum water vapor threshold, likely case dependent, for producing liquid water clouds. Through responses to the radiative forcing and surface warming, this cloud liquid water also modulates the turbulent and conductive heat fluxes, and produces a thermal wave penetrating into the sea ice. About 20-33 % of the observed variations of bottom ice growth can be directly linked to variations in surface conductive heat flux, with retarded ice growth occurring several days after these moisture plumes reduce the surface conductive heat flux. This sequence of events modulate pack-ice wintertime environmental conditions and total ice growth, and has implications for the annual sea-ice evolution, especially for the current conditions of extensive thinner ice.

Generating code adapted for interlinking legacy scalar code and extended vector code

DOEpatents

Gschwind, Michael K

2013-06-04

Mechanisms for intermixing code are provided. Source code is received for compilation using an extended Application Binary Interface (ABI) that extends a legacy ABI and uses a different register configuration than the legacy ABI. First compiled code is generated based on the source code, the first compiled code comprising code for accommodating the difference in register configurations used by the extended ABI and the legacy ABI. The first compiled code and second compiled code are intermixed to generate intermixed code, the second compiled code being compiled code that uses the legacy ABI. The intermixed code comprises at least one call instruction that is one of a call from the first compiled code to the second compiled code or a call from the second compiled code to the first compiled code. The code for accommodating the difference in register configurations is associated with the at least one call instruction.

Methotrexate loaded polyether-copolyester dendrimers for the treatment of gliomas: enhanced efficacy and intratumoral transport capability.

PubMed

Dhanikula, Renu Singh; Argaw, Anteneh; Bouchard, Jean-Francois; Hildgen, Patrice

2008-01-01

Therapeutic benefit in glial tumors is often limited due to low permeability of delivery systems across the blood-brain barrier (BBB), drug resistance, and poor penetration into the tumor tissue. In an attempt to overcome these hurdles, polyether-copolyester (PEPE) dendrimers were evaluated as drug carriers for the treatment of gliomas. Dendrimers were conjugated to d-glucosamine as the ligand for enhancing BBB permeability and tumor targeting. The efficacy of methotrexate (MTX)-loaded dendrimers was established against U87 MG and U 343 MGa cells. Permeability of rhodamine-labeled dendrimers and MTX-loaded dendrimers across the in vitro BBB model and their distribution into avascular human glioma tumor spheroids was also studied. Glucosylated dendrimers were found to be endocytosed in significantly higher amounts than nonglucosylated dendrimers by both the cell lines. IC 50 of MTX after loading in dendrimers was lower than that of the free MTX, suggesting that loading MTX in PEPE dendrimers increased its potency. Similar higher activity of MTX-loaded glucosylated and nonglucosylated dendrimers was found in the reduction of tumor spheroid size. These MTX-loaded dendrimers were able to kill even MTX-resistant cells highlighting their ability to overcome MTX resistance. In addition, the amount of MTX-transported across BBB was three to five times more after loading in the dendrimers. Glucosylation further increased the cumulative permeation of dendrimers across BBB and hence increased the amount of MTX available across it. Glucosylated dendrimers distributed through out the avascular tumor spheroids within 6 h, while nonglucosylated dendrimers could do so in 12 h. The results show that glucosamine can be used as an effective ligand not only for targeting glial tumors but also for enhanced permeability across BBB. Thus, glucosylated PEPE dendrimers can serve as potential delivery system for the treatment of gliomas.

The 3of5 web application for complex and comprehensive pattern matching in protein sequences.

PubMed

Seiler, Markus; Mehrle, Alexander; Poustka, Annemarie; Wiemann, Stefan

2006-03-16

The identification of patterns in biological sequences is a key challenge in genome analysis and in proteomics. Frequently such patterns are complex and highly variable, especially in protein sequences. They are frequently described using terms of regular expressions (RegEx) because of the user-friendly terminology. Limitations arise for queries with the increasing complexity of patterns and are accompanied by requirements for enhanced capabilities. This is especially true for patterns containing ambiguous characters and positions and/or length ambiguities. We have implemented the 3of5 web application in order to enable complex pattern matching in protein sequences. 3of5 is named after a special use of its main feature, the novel n-of-m pattern type. This feature allows for an extensive specification of variable patterns where the individual elements may vary in their position, order, and content within a defined stretch of sequence. The number of distinct elements can be constrained by operators, and individual characters may be excluded. The n-of-m pattern type can be combined with common regular expression terms and thus also allows for a comprehensive description of complex patterns. 3of5 increases the fidelity of pattern matching and finds ALL possible solutions in protein sequences in cases of length-ambiguous patterns instead of simply reporting the longest or shortest hits. Grouping and combined search for patterns provides a hierarchical arrangement of larger patterns sets. The algorithm is implemented as internet application and freely accessible. The application is available at http://dkfz.de/mga2/3of5/3of5.html. The 3of5 application offers an extended vocabulary for the definition of search patterns and thus allows the user to comprehensively specify and identify peptide patterns with variable elements. The n-of-m pattern type offers an improved accuracy for pattern matching in combination with the ability to find all solutions, without compromising the user friendliness of regular expression terms.

Teplizumab for treatment of type 1 diabetes mellitus.

PubMed

Skelley, Jessica W; Elmore, Lindsey K; Kyle, Jeffrey A

2012-10-01

To review the pharmacology, pharmacokinetics, safety, and efficacy of teplizumab and evaluate relevant clinical trial data. Searches of MEDLINE, International Pharmaceutical Abstracts, ClinicalTrials.gov, American Diabetes Association scientific posters, and Google Scholar (1966-May 2012) were conducted using the key words teplizumab, anti-CD3 monoclonal antibody, MGA031, and hOKT3γ1 (Ala-Ala). Searches were limited to articles published in English. Clinical trials evaluating teplizumab for type 1 diabetes mellitus (T1DM) published in English were selected from the data sources. All published relevant abstracts were included. References cited in identified articles were used for additional citations. T1DM accounts for up to 10% of all cases of diabetes mellitus. T1DM is characterized as a chronic and progressive autoimmune disease leading to the destruction of insulin-producing β-cells of the pancreas. Teplizumab is a humanized Fc-mutated anti-CD3 monoclonal antibody that alters the function of the T-lymphocytes that mediate the destruction of the insulin-producing β-cells. While clinical data are limited, both Phase 2 and Phase 3 studies have demonstrated preserved C-peptide response as a measure of insulin production, decreased exogenous insulin use, and improved glycemic control following a 12- to 14-day teplizumab infusion in patients diagnosed with T1DM within the previous 6 weeks. However, 1 Phase 3 trial failed to find the same benefits in those diagnosed with T1DM within the previous 12 weeks when a lower cumulative teplizumab dose was used. Initial studies indicated that teplizumab is well tolerated, with a self-limiting rash as the most commonly reported adverse effect. Teplizumab is an anti-CD3 human monoclonal antibody with promising activity in treatment of patients with T1DM. Results from Phase 3 trials are needed to further determine safety, efficacy, and dosing frequency.

Bandwidth efficient coding for satellite communications

NASA Technical Reports Server (NTRS)

Lin, Shu; Costello, Daniel J., Jr.; Miller, Warner H.; Morakis, James C.; Poland, William B., Jr.

1992-01-01

An error control coding scheme was devised to achieve large coding gain and high reliability by using coded modulation with reduced decoding complexity. To achieve a 3 to 5 dB coding gain and moderate reliability, the decoding complexity is quite modest. In fact, to achieve a 3 dB coding gain, the decoding complexity is quite simple, no matter whether trellis coded modulation or block coded modulation is used. However, to achieve coding gains exceeding 5 dB, the decoding complexity increases drastically, and the implementation of the decoder becomes very expensive and unpractical. The use is proposed of coded modulation in conjunction with concatenated (or cascaded) coding. A good short bandwidth efficient modulation code is used as the inner code and relatively powerful Reed-Solomon code is used as the outer code. With properly chosen inner and outer codes, a concatenated coded modulation scheme not only can achieve large coding gains and high reliability with good bandwidth efficiency but also can be practically implemented. This combination of coded modulation and concatenated coding really offers a way of achieving the best of three worlds, reliability and coding gain, bandwidth efficiency, and decoding complexity.

A genetic scale of reading frame coding.

PubMed

Michel, Christian J

2014-08-21

The reading frame coding (RFC) of codes (sets) of trinucleotides is a genetic concept which has been largely ignored during the last 50 years. A first objective is the definition of a new and simple statistical parameter PrRFC for analysing the probability (efficiency) of reading frame coding (RFC) of any trinucleotide code. A second objective is to reveal different classes and subclasses of trinucleotide codes involved in reading frame coding: the circular codes of 20 trinucleotides and the bijective genetic codes of 20 trinucleotides coding the 20 amino acids. This approach allows us to propose a genetic scale of reading frame coding which ranges from 1/3 with the random codes (RFC probability identical in the three frames) to 1 with the comma-free circular codes (RFC probability maximal in the reading frame and null in the two shifted frames). This genetic scale shows, in particular, the reading frame coding probabilities of the 12,964,440 circular codes (PrRFC=83.2% in average), the 216 C(3) self-complementary circular codes (PrRFC=84.1% in average) including the code X identified in eukaryotic and prokaryotic genes (PrRFC=81.3%) and the 339,738,624 bijective genetic codes (PrRFC=61.5% in average) including the 52 codes without permuted trinucleotides (PrRFC=66.0% in average). Otherwise, the reading frame coding probabilities of each trinucleotide code coding an amino acid with the universal genetic code are also determined. The four amino acids Gly, Lys, Phe and Pro are coded by codes (not circular) with RFC probabilities equal to 2/3, 1/2, 1/2 and 2/3, respectively. The amino acid Leu is coded by a circular code (not comma-free) with a RFC probability equal to 18/19. The 15 other amino acids are coded by comma-free circular codes, i.e. with RFC probabilities equal to 1. The identification of coding properties in some classes of trinucleotide codes studied here may bring new insights in the origin and evolution of the genetic code. Copyright © 2014 Elsevier Ltd. All rights reserved.

Error-correction coding for digital communications

NASA Astrophysics Data System (ADS)

Clark, G. C., Jr.; Cain, J. B.

This book is written for the design engineer who must build the coding and decoding equipment and for the communication system engineer who must incorporate this equipment into a system. It is also suitable as a senior-level or first-year graduate text for an introductory one-semester course in coding theory. Fundamental concepts of coding are discussed along with group codes, taking into account basic principles, practical constraints, performance computations, coding bounds, generalized parity check codes, polynomial codes, and important classes of group codes. Other topics explored are related to simple nonalgebraic decoding techniques for group codes, soft decision decoding of block codes, algebraic techniques for multiple error correction, the convolutional code structure and Viterbi decoding, syndrome decoding techniques, and sequential decoding techniques. System applications are also considered, giving attention to concatenated codes, coding for the white Gaussian noise channel, interleaver structures for coded systems, and coding for burst noise channels.

A novel concatenated code based on the improved SCG-LDPC code for optical transmission systems

NASA Astrophysics Data System (ADS)

Yuan, Jian-guo; Xie, Ya; Wang, Lin; Huang, Sheng; Wang, Yong

2013-01-01

Based on the optimization and improvement for the construction method of systematically constructed Gallager (SCG) (4, k) code, a novel SCG low density parity check (SCG-LDPC)(3969, 3720) code to be suitable for optical transmission systems is constructed. The novel SCG-LDPC (6561,6240) code with code rate of 95.1% is constructed by increasing the length of SCG-LDPC (3969,3720) code, and in a way, the code rate of LDPC codes can better meet the high requirements of optical transmission systems. And then the novel concatenated code is constructed by concatenating SCG-LDPC(6561,6240) code and BCH(127,120) code with code rate of 94.5%. The simulation results and analyses show that the net coding gain (NCG) of BCH(127,120)+SCG-LDPC(6561,6240) concatenated code is respectively 2.28 dB and 0.48 dB more than those of the classic RS(255,239) code and SCG-LDPC(6561,6240) code at the bit error rate (BER) of 10-7.

Trace-shortened Reed-Solomon codes

NASA Technical Reports Server (NTRS)

Mceliece, R. J.; Solomon, G.

1994-01-01

Reed-Solomon (RS) codes have been part of standard NASA telecommunications systems for many years. RS codes are character-oriented error-correcting codes, and their principal use in space applications has been as outer codes in concatenated coding systems. However, for a given character size, say m bits, RS codes are limited to a length of, at most, 2(exp m). It is known in theory that longer character-oriented codes would be superior to RS codes in concatenation applications, but until recently no practical class of 'long' character-oriented codes had been discovered. In 1992, however, Solomon discovered an extensive class of such codes, which are now called trace-shortened Reed-Solomon (TSRS) codes. In this article, we will continue the study of TSRS codes. Our main result is a formula for the dimension of any TSRS code, as a function of its error-correcting power. Using this formula, we will give several examples of TSRS codes, some of which look very promising as candidate outer codes in high-performance coded telecommunications systems.

Error Control Coding Techniques for Space and Satellite Communications

NASA Technical Reports Server (NTRS)

Lin, Shu

2000-01-01

This paper presents a concatenated turbo coding system in which a Reed-Solomom outer code is concatenated with a binary turbo inner code. In the proposed system, the outer code decoder and the inner turbo code decoder interact to achieve both good bit error and frame error performances. The outer code decoder helps the inner turbo code decoder to terminate its decoding iteration while the inner turbo code decoder provides soft-output information to the outer code decoder to carry out a reliability-based soft-decision decoding. In the case that the outer code decoding fails, the outer code decoder instructs the inner code decoder to continue its decoding iterations until the outer code decoding is successful or a preset maximum number of decoding iterations is reached. This interaction between outer and inner code decoders reduces decoding delay. Also presented in the paper are an effective criterion for stopping the iteration process of the inner code decoder and a new reliability-based decoding algorithm for nonbinary codes.

An Interactive Concatenated Turbo Coding System

NASA Technical Reports Server (NTRS)

Liu, Ye; Tang, Heng; Lin, Shu; Fossorier, Marc

1999-01-01

This paper presents a concatenated turbo coding system in which a Reed-Solomon outer code is concatenated with a binary turbo inner code. In the proposed system, the outer code decoder and the inner turbo code decoder interact to achieve both good bit error and frame error performances. The outer code decoder helps the inner turbo code decoder to terminate its decoding iteration while the inner turbo code decoder provides soft-output information to the outer code decoder to carry out a reliability-based soft- decision decoding. In the case that the outer code decoding fails, the outer code decoder instructs the inner code decoder to continue its decoding iterations until the outer code decoding is successful or a preset maximum number of decoding iterations is reached. This interaction between outer and inner code decoders reduces decoding delay. Also presented in the paper are an effective criterion for stopping the iteration process of the inner code decoder and a new reliability-based decoding algorithm for nonbinary codes.

Performance Analysis of New Binary User Codes for DS-CDMA Communication

NASA Astrophysics Data System (ADS)

Usha, Kamle; Jaya Sankar, Kottareddygari

2016-03-01

This paper analyzes new binary spreading codes through correlation properties and also presents their performance over additive white Gaussian noise (AWGN) channel. The proposed codes are constructed using gray and inverse gray codes. In this paper, a n-bit gray code appended by its n-bit inverse gray code to construct the 2n-length binary user codes are discussed. Like Walsh codes, these binary user codes are available in sizes of power of two and additionally code sets of length 6 and their even multiples are also available. The simple construction technique and generation of code sets of different sizes are the salient features of the proposed codes. Walsh codes and gold codes are considered for comparison in this paper as these are popularly used for synchronous and asynchronous multi user communications respectively. In the current work the auto and cross correlation properties of the proposed codes are compared with those of Walsh codes and gold codes. Performance of the proposed binary user codes for both synchronous and asynchronous direct sequence CDMA communication over AWGN channel is also discussed in this paper. The proposed binary user codes are found to be suitable for both synchronous and asynchronous DS-CDMA communication.

Multiple component codes based generalized LDPC codes for high-speed optical transport.

PubMed

Djordjevic, Ivan B; Wang, Ting

2014-07-14

A class of generalized low-density parity-check (GLDPC) codes suitable for optical communications is proposed, which consists of multiple local codes. It is shown that Hamming, BCH, and Reed-Muller codes can be used as local codes, and that the maximum a posteriori probability (MAP) decoding of these local codes by Ashikhmin-Lytsin algorithm is feasible in terms of complexity and performance. We demonstrate that record coding gains can be obtained from properly designed GLDPC codes, derived from multiple component codes. We then show that several recently proposed classes of LDPC codes such as convolutional and spatially-coupled codes can be described using the concept of GLDPC coding, which indicates that the GLDPC coding can be used as a unified platform for advanced FEC enabling ultra-high speed optical transport. The proposed class of GLDPC codes is also suitable for code-rate adaption, to adjust the error correction strength depending on the optical channel conditions.

Multilevel Concatenated Block Modulation Codes for the Frequency Non-selective Rayleigh Fading Channel

NASA Technical Reports Server (NTRS)

Lin, Shu; Rhee, Dojun

1996-01-01

This paper is concerned with construction of multilevel concatenated block modulation codes using a multi-level concatenation scheme for the frequency non-selective Rayleigh fading channel. In the construction of multilevel concatenated modulation code, block modulation codes are used as the inner codes. Various types of codes (block or convolutional, binary or nonbinary) are being considered as the outer codes. In particular, we focus on the special case for which Reed-Solomon (RS) codes are used as the outer codes. For this special case, a systematic algebraic technique for constructing q-level concatenated block modulation codes is proposed. Codes have been constructed for certain specific values of q and compared with the single-level concatenated block modulation codes using the same inner codes. A multilevel closest coset decoding scheme for these codes is proposed.

Unaligned instruction relocation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bertolli, Carlo; O'Brien, John K.; Sallenave, Olivier H.

In one embodiment, a computer-implemented method includes receiving source code to be compiled into an executable file for an unaligned instruction set architecture (ISA). Aligned assembled code is generated, by a computer processor. The aligned assembled code complies with an aligned ISA and includes aligned processor code for a processor and aligned accelerator code for an accelerator. A first linking pass is performed on the aligned assembled code, including relocating a first relocation target in the aligned accelerator code that refers to a first object outside the aligned accelerator code. Unaligned assembled code is generated in accordance with the unalignedmore » ISA and includes unaligned accelerator code for the accelerator and unaligned processor code for the processor. A second linking pass is performed on the unaligned assembled code, including relocating a second relocation target outside the unaligned accelerator code that refers to an object in the unaligned accelerator code.« less

Unaligned instruction relocation

DOEpatents

Bertolli, Carlo; O'Brien, John K.; Sallenave, Olivier H.; Sura, Zehra N.

2018-01-23

In one embodiment, a computer-implemented method includes receiving source code to be compiled into an executable file for an unaligned instruction set architecture (ISA). Aligned assembled code is generated, by a computer processor. The aligned assembled code complies with an aligned ISA and includes aligned processor code for a processor and aligned accelerator code for an accelerator. A first linking pass is performed on the aligned assembled code, including relocating a first relocation target in the aligned accelerator code that refers to a first object outside the aligned accelerator code. Unaligned assembled code is generated in accordance with the unaligned ISA and includes unaligned accelerator code for the accelerator and unaligned processor code for the processor. A second linking pass is performed on the unaligned assembled code, including relocating a second relocation target outside the unaligned accelerator code that refers to an object in the unaligned accelerator code.

Linear chirp phase perturbing approach for finding binary phased codes

NASA Astrophysics Data System (ADS)

Li, Bing C.

2017-05-01

Binary phased codes have many applications in communication and radar systems. These applications require binary phased codes to have low sidelobes in order to reduce interferences and false detection. Barker codes are the ones that satisfy these requirements and they have lowest maximum sidelobes. However, Barker codes have very limited code lengths (equal or less than 13) while many applications including low probability of intercept radar, and spread spectrum communication, require much higher code lengths. The conventional techniques of finding binary phased codes in literatures include exhaust search, neural network, and evolutionary methods, and they all require very expensive computation for large code lengths. Therefore these techniques are limited to find binary phased codes with small code lengths (less than 100). In this paper, by analyzing Barker code, linear chirp, and P3 phases, we propose a new approach to find binary codes. Experiments show that the proposed method is able to find long low sidelobe binary phased codes (code length >500) with reasonable computational cost.

Maximum likelihood decoding analysis of Accumulate-Repeat-Accumulate Codes

NASA Technical Reports Server (NTRS)

Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

2004-01-01

Repeat-Accumulate (RA) codes are the simplest turbo-like codes that achieve good performance. However, they cannot compete with Turbo codes or low-density parity check codes (LDPC) as far as performance is concerned. The Accumulate Repeat Accumulate (ARA) codes, as a subclass of LDPC codes, are obtained by adding a pre-coder in front of RA codes with puncturing where an accumulator is chosen as a precoder. These codes not only are very simple, but also achieve excellent performance with iterative decoding. In this paper, the performance of these codes with (ML) decoding are analyzed and compared to random codes by very tight bounds. The weight distribution of some simple ARA codes is obtained, and through existing tightest bounds we have shown the ML SNR threshold of ARA codes approaches very closely to the performance of random codes. We have shown that the use of precoder improves the SNR threshold but interleaving gain remains unchanged with respect to RA code with puncturing.

Accumulate repeat accumulate codes

NASA Technical Reports Server (NTRS)

Abbasfar, A.; Divsalar, D.; Yao, K.

2004-01-01

In this paper we propose an innovative channel coding scheme called Accumulate Repeat Accumulate codes. This class of codes can be viewed as trubo-like codes, namely a double serial concatenation of a rate-1 accumulator as an outer code, a regular or irregular repetition as a middle code, and a punctured accumulator as an inner code.

Ensemble coding of face identity is not independent of the coding of individual identity.

PubMed

Neumann, Markus F; Ng, Ryan; Rhodes, Gillian; Palermo, Romina

2018-06-01

Information about a group of similar objects can be summarized into a compressed code, known as ensemble coding. Ensemble coding of simple stimuli (e.g., groups of circles) can occur in the absence of detailed exemplar coding, suggesting dissociable processes. Here, we investigate whether a dissociation would still be apparent when coding facial identity, where individual exemplar information is much more important. We examined whether ensemble coding can occur when exemplar coding is difficult, as a result of large sets or short viewing times, or whether the two types of coding are positively associated. We found a positive association, whereby both ensemble and exemplar coding were reduced for larger groups and shorter viewing times. There was no evidence for ensemble coding in the absence of exemplar coding. At longer presentation times, there was an unexpected dissociation, where exemplar coding increased yet ensemble coding decreased, suggesting that robust information about face identity might suppress ensemble coding. Thus, for face identity, we did not find the classic dissociation-of access to ensemble information in the absence of detailed exemplar information-that has been used to support claims of distinct mechanisms for ensemble and exemplar coding.

Performance and structure of single-mode bosonic codes

NASA Astrophysics Data System (ADS)

Albert, Victor V.; Noh, Kyungjoo; Duivenvoorden, Kasper; Young, Dylan J.; Brierley, R. T.; Reinhold, Philip; Vuillot, Christophe; Li, Linshu; Shen, Chao; Girvin, S. M.; Terhal, Barbara M.; Jiang, Liang

2018-03-01

The early Gottesman, Kitaev, and Preskill (GKP) proposal for encoding a qubit in an oscillator has recently been followed by cat- and binomial-code proposals. Numerically optimized codes have also been proposed, and we introduce codes of this type here. These codes have yet to be compared using the same error model; we provide such a comparison by determining the entanglement fidelity of all codes with respect to the bosonic pure-loss channel (i.e., photon loss) after the optimal recovery operation. We then compare achievable communication rates of the combined encoding-error-recovery channel by calculating the channel's hashing bound for each code. Cat and binomial codes perform similarly, with binomial codes outperforming cat codes at small loss rates. Despite not being designed to protect against the pure-loss channel, GKP codes significantly outperform all other codes for most values of the loss rate. We show that the performance of GKP and some binomial codes increases monotonically with increasing average photon number of the codes. In order to corroborate our numerical evidence of the cat-binomial-GKP order of performance occurring at small loss rates, we analytically evaluate the quantum error-correction conditions of those codes. For GKP codes, we find an essential singularity in the entanglement fidelity in the limit of vanishing loss rate. In addition to comparing the codes, we draw parallels between binomial codes and discrete-variable systems. First, we characterize one- and two-mode binomial as well as multiqubit permutation-invariant codes in terms of spin-coherent states. Such a characterization allows us to introduce check operators and error-correction procedures for binomial codes. Second, we introduce a generalization of spin-coherent states, extending our characterization to qudit binomial codes and yielding a multiqudit code.

LDPC Codes with Minimum Distance Proportional to Block Size

NASA Technical Reports Server (NTRS)

Divsalar, Dariush; Jones, Christopher; Dolinar, Samuel; Thorpe, Jeremy

2009-01-01

Low-density parity-check (LDPC) codes characterized by minimum Hamming distances proportional to block sizes have been demonstrated. Like the codes mentioned in the immediately preceding article, the present codes are error-correcting codes suitable for use in a variety of wireless data-communication systems that include noisy channels. The previously mentioned codes have low decoding thresholds and reasonably low error floors. However, the minimum Hamming distances of those codes do not grow linearly with code-block sizes. Codes that have this minimum-distance property exhibit very low error floors. Examples of such codes include regular LDPC codes with variable degrees of at least 3. Unfortunately, the decoding thresholds of regular LDPC codes are high. Hence, there is a need for LDPC codes characterized by both low decoding thresholds and, in order to obtain acceptably low error floors, minimum Hamming distances that are proportional to code-block sizes. The present codes were developed to satisfy this need. The minimum Hamming distances of the present codes have been shown, through consideration of ensemble-average weight enumerators, to be proportional to code block sizes. As in the cases of irregular ensembles, the properties of these codes are sensitive to the proportion of degree-2 variable nodes. A code having too few such nodes tends to have an iterative decoding threshold that is far from the capacity threshold. A code having too many such nodes tends not to exhibit a minimum distance that is proportional to block size. Results of computational simulations have shown that the decoding thresholds of codes of the present type are lower than those of regular LDPC codes. Included in the simulations were a few examples from a family of codes characterized by rates ranging from low to high and by thresholds that adhere closely to their respective channel capacity thresholds; the simulation results from these examples showed that the codes in question have low error floors as well as low decoding thresholds. As an example, the illustration shows the protograph (which represents the blueprint for overall construction) of one proposed code family for code rates greater than or equal to 1.2. Any size LDPC code can be obtained by copying the protograph structure N times, then permuting the edges. The illustration also provides Field Programmable Gate Array (FPGA) hardware performance simulations for this code family. In addition, the illustration provides minimum signal-to-noise ratios (Eb/No) in decibels (decoding thresholds) to achieve zero error rates as the code block size goes to infinity for various code rates. In comparison with the codes mentioned in the preceding article, these codes have slightly higher decoding thresholds.

76 FR 77549 - Lummi Nation-Title 20-Code of Laws-Liquor Code

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-13

... DEPARTMENT OF THE INTERIOR Bureau of Indian Affairs Lummi Nation--Title 20--Code of Laws--Liquor... amendment to Lummi Nation's Title 20--Code of Laws--Liquor Code. The Code regulates and controls the... this amendment to Title 20--Lummi Nation Code of Laws--Liquor Code by Resolution 2011-038 on March 1...

Error-correction coding

NASA Technical Reports Server (NTRS)

Hinds, Erold W. (Principal Investigator)

1996-01-01

This report describes the progress made towards the completion of a specific task on error-correcting coding. The proposed research consisted of investigating the use of modulation block codes as the inner code of a concatenated coding system in order to improve the overall space link communications performance. The study proposed to identify and analyze candidate codes that will complement the performance of the overall coding system which uses the interleaved RS (255,223) code as the outer code.

Beam-dynamics codes used at DARHT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ekdahl, Jr., Carl August

Several beam simulation codes are used to help gain a better understanding of beam dynamics in the DARHT LIAs. The most notable of these fall into the following categories: for beam production – Tricomp Trak orbit tracking code, LSP Particle in cell (PIC) code, for beam transport and acceleration – XTR static envelope and centroid code, LAMDA time-resolved envelope and centroid code, LSP-Slice PIC code, for coasting-beam transport to target – LAMDA time-resolved envelope code, LSP-Slice PIC code. These codes are also being used to inform the design of Scorpius.

Amino acid codes in mitochondria as possible clues to primitive codes

NASA Technical Reports Server (NTRS)

Jukes, T. H.

1981-01-01

Differences between mitochondrial codes and the universal code indicate that an evolutionary simplification has taken place, rather than a return to a more primitive code. However, these differences make it evident that the universal code is not the only code possible, and therefore earlier codes may have differed markedly from the previous code. The present universal code is probably a 'frozen accident.' The change in CUN codons from leucine to threonine (Neurospora vs. yeast mitochondria) indicates that neutral or near-neutral changes occurred in the corresponding proteins when this code change took place, caused presumably by a mutation in a tRNA gene.

Some partial-unit-memory convolutional codes

NASA Technical Reports Server (NTRS)

Abdel-Ghaffar, K.; Mceliece, R. J.; Solomon, G.

1991-01-01

The results of a study on a class of error correcting codes called partial unit memory (PUM) codes are presented. This class of codes, though not entirely new, has until now remained relatively unexplored. The possibility of using the well developed theory of block codes to construct a large family of promising PUM codes is shown. The performance of several specific PUM codes are compared with that of the Voyager standard (2, 1, 6) convolutional code. It was found that these codes can outperform the Voyager code with little or no increase in decoder complexity. This suggests that there may very well be PUM codes that can be used for deep space telemetry that offer both increased performance and decreased implementational complexity over current coding systems.

Concatenated coding systems employing a unit-memory convolutional code and a byte-oriented decoding algorithm

NASA Technical Reports Server (NTRS)

Lee, L.-N.

1977-01-01

Concatenated coding systems utilizing a convolutional code as the inner code and a Reed-Solomon code as the outer code are considered. In order to obtain very reliable communications over a very noisy channel with relatively modest coding complexity, it is proposed to concatenate a byte-oriented unit-memory convolutional code with an RS outer code whose symbol size is one byte. It is further proposed to utilize a real-time minimal-byte-error probability decoding algorithm, together with feedback from the outer decoder, in the decoder for the inner convolutional code. The performance of the proposed concatenated coding system is studied, and the improvement over conventional concatenated systems due to each additional feature is isolated.

Concatenated coding systems employing a unit-memory convolutional code and a byte-oriented decoding algorithm

NASA Technical Reports Server (NTRS)

Lee, L. N.

1976-01-01

Concatenated coding systems utilizing a convolutional code as the inner code and a Reed-Solomon code as the outer code are considered. In order to obtain very reliable communications over a very noisy channel with relatively small coding complexity, it is proposed to concatenate a byte oriented unit memory convolutional code with an RS outer code whose symbol size is one byte. It is further proposed to utilize a real time minimal byte error probability decoding algorithm, together with feedback from the outer decoder, in the decoder for the inner convolutional code. The performance of the proposed concatenated coding system is studied, and the improvement over conventional concatenated systems due to each additional feature is isolated.

You've Written a Cool Astronomy Code! Now What Do You Do with It?

NASA Astrophysics Data System (ADS)

Allen, Alice; Accomazzi, A.; Berriman, G. B.; DuPrie, K.; Hanisch, R. J.; Mink, J. D.; Nemiroff, R. J.; Shamir, L.; Shortridge, K.; Taylor, M. B.; Teuben, P. J.; Wallin, J. F.

2014-01-01

Now that you've written a useful astronomy code for your soon-to-be-published research, you have to figure out what you want to do with it. Our suggestion? Share it! This presentation highlights the means and benefits of sharing your code. Make your code citable -- submit it to the Astrophysics Source Code Library and have it indexed by ADS! The Astrophysics Source Code Library (ASCL) is a free online registry of source codes of interest to astronomers and astrophysicists. With over 700 codes, it is continuing its rapid growth, with an average of 17 new codes a month. The editors seek out codes for inclusion; indexing by ADS improves the discoverability of codes and provides a way to cite codes as separate entries, especially codes without papers that describe them.

Box codes of lengths 48 and 72

NASA Technical Reports Server (NTRS)

Solomon, G.; Jin, Y.

1993-01-01

A self-dual code length 48, dimension 24, with Hamming distance essentially equal to 12 is constructed here. There are only six code words of weight eight. All the other code words have weights that are multiples of four and have a minimum weight equal to 12. This code may be encoded systematically and arises from a strict binary representation of the (8,4;5) Reed-Solomon (RS) code over GF (64). The code may be considered as six interrelated (8,7;2) codes. The Mattson-Solomon representation of the cyclic decomposition of these codes and their parity sums are used to detect an odd number of errors in any of the six codes. These may then be used in a correction algorithm for hard or soft decision decoding. A (72,36;15) box code was constructed from a (63,35;8) cyclic code. The theoretical justification is presented herein. A second (72,36;15) code is constructed from an inner (63,27;16) Bose Chaudhuri Hocquenghem (BCH) code and expanded to length 72 using box code algorithms for extension. This code was simulated and verified to have a minimum distance of 15 with even weight words congruent to zero modulo four. The decoding for hard and soft decision is still more complex than the first code constructed above. Finally, an (8,4;5) RS code over GF (512) in the binary representation of the (72,36;15) box code gives rise to a (72,36;16*) code with nine words of weight eight, and all the rest have weights greater than or equal to 16.

Error control techniques for satellite and space communications

NASA Technical Reports Server (NTRS)

Costello, Daniel J., Jr.

1989-01-01

Two aspects of the work for NASA are examined: the construction of multi-dimensional phase modulation trellis codes and a performance analysis of these codes. A complete list is contained of all the best trellis codes for use with phase modulation. LxMPSK signal constellations are included for M = 4, 8, and 16 and L = 1, 2, 3, and 4. Spectral efficiencies range from 1 bit/channel symbol (equivalent to rate 1/2 coded QPSK) to 3.75 bits/channel symbol (equivalent to 15/16 coded 16-PSK). The parity check polynomials, rotational invariance properties, free distance, path multiplicities, and coding gains are given for all codes. These codes are considered to be the best candidates for implementation of a high speed decoder for satellite transmission. The design of a hardware decoder for one of these codes, viz., the 16-state 3x8-PSK code with free distance 4.0 and coding gain 3.75 dB is discussed. An exhaustive simulation study of the multi-dimensional phase modulation trellis codes is contained. This study was motivated by the fact that coding gains quoted for almost all codes found in literature are in fact only asymptotic coding gains, i.e., the coding gain at very high signal to noise ratios (SNRs) or very low BER. These asymptotic coding gains can be obtained directly from a knowledge of the free distance of the code. On the other hand, real coding gains at BERs in the range of 10(exp -2) to 10(exp -6), where these codes are most likely to operate in a concatenated system, must be done by simulation.

Accumulate repeat accumulate codes

NASA Technical Reports Server (NTRS)

Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

2004-01-01

In this paper we propose an innovative channel coding scheme called 'Accumulate Repeat Accumulate codes' (ARA). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes, thus belief propagation can be used for iterative decoding of ARA codes on a graph. The structure of encoder for this class can be viewed as precoded Repeat Accumulate (RA) code or as precoded Irregular Repeat Accumulate (IRA) code, where simply an accumulator is chosen as a precoder. Thus ARA codes have simple, and very fast encoder structure when they representing LDPC codes. Based on density evolution for LDPC codes through some examples for ARA codes, we show that for maximum variable node degree 5 a minimum bit SNR as low as 0.08 dB from channel capacity for rate 1/2 can be achieved as the block size goes to infinity. Thus based on fixed low maximum variable node degree, its threshold outperforms not only the RA and IRA codes but also the best known LDPC codes with the dame maximum node degree. Furthermore by puncturing the accumulators any desired high rate codes close to code rate 1 can be obtained with thresholds that stay close to the channel capacity thresholds uniformly. Iterative decoding simulation results are provided. The ARA codes also have projected graph or protograph representation that allows for high speed decoder implementation.

Combinatorial neural codes from a mathematical coding theory perspective.

PubMed

Curto, Carina; Itskov, Vladimir; Morrison, Katherine; Roth, Zachary; Walker, Judy L

2013-07-01

Shannon's seminal 1948 work gave rise to two distinct areas of research: information theory and mathematical coding theory. While information theory has had a strong influence on theoretical neuroscience, ideas from mathematical coding theory have received considerably less attention. Here we take a new look at combinatorial neural codes from a mathematical coding theory perspective, examining the error correction capabilities of familiar receptive field codes (RF codes). We find, perhaps surprisingly, that the high levels of redundancy present in these codes do not support accurate error correction, although the error-correcting performance of receptive field codes catches up to that of random comparison codes when a small tolerance to error is introduced. However, receptive field codes are good at reflecting distances between represented stimuli, while the random comparison codes are not. We suggest that a compromise in error-correcting capability may be a necessary price to pay for a neural code whose structure serves not only error correction, but must also reflect relationships between stimuli.

Joint design of QC-LDPC codes for coded cooperation system with joint iterative decoding

NASA Astrophysics Data System (ADS)

Zhang, Shunwai; Yang, Fengfan; Tang, Lei; Ejaz, Saqib; Luo, Lin; Maharaj, B. T.

2016-03-01

In this paper, we investigate joint design of quasi-cyclic low-density-parity-check (QC-LDPC) codes for coded cooperation system with joint iterative decoding in the destination. First, QC-LDPC codes based on the base matrix and exponent matrix are introduced, and then we describe two types of girth-4 cycles in QC-LDPC codes employed by the source and relay. In the equivalent parity-check matrix corresponding to the jointly designed QC-LDPC codes employed by the source and relay, all girth-4 cycles including both type I and type II are cancelled. Theoretical analysis and numerical simulations show that the jointly designed QC-LDPC coded cooperation well combines cooperation gain and channel coding gain, and outperforms the coded non-cooperation under the same conditions. Furthermore, the bit error rate performance of the coded cooperation employing jointly designed QC-LDPC codes is better than those of random LDPC codes and separately designed QC-LDPC codes over AWGN channels.

Accumulate-Repeat-Accumulate-Accumulate Codes

NASA Technical Reports Server (NTRS)

Divsalar, Dariush; Dolinar, Samuel; Thorpe, Jeremy

2007-01-01

Accumulate-repeat-accumulate-accumulate (ARAA) codes have been proposed, inspired by the recently proposed accumulate-repeat-accumulate (ARA) codes. These are error-correcting codes suitable for use in a variety of wireless data-communication systems that include noisy channels. ARAA codes can be regarded as serial turbolike codes or as a subclass of low-density parity-check (LDPC) codes, and, like ARA codes they have projected graph or protograph representations; these characteristics make it possible to design high-speed iterative decoders that utilize belief-propagation algorithms. The objective in proposing ARAA codes as a subclass of ARA codes was to enhance the error-floor performance of ARA codes while maintaining simple encoding structures and low maximum variable node degree.

The EDIT-COMGEOM Code

DTIC Science & Technology

1975-09-01

This report assumes a familiarity with the GIFT and MAGIC computer codes. The EDIT-COMGEOM code is a FORTRAN computer code. The EDIT-COMGEOM code...converts the target description data which was used in the MAGIC computer code to the target description data which can be used in the GIFT computer code

Impacts of Model Building Energy Codes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Athalye, Rahul A.; Sivaraman, Deepak; Elliott, Douglas B.

The U.S. Department of Energy (DOE) Building Energy Codes Program (BECP) periodically evaluates national and state-level impacts associated with energy codes in residential and commercial buildings. Pacific Northwest National Laboratory (PNNL), funded by DOE, conducted an assessment of the prospective impacts of national model building energy codes from 2010 through 2040. A previous PNNL study evaluated the impact of the Building Energy Codes Program; this study looked more broadly at overall code impacts. This report describes the methodology used for the assessment and presents the impacts in terms of energy savings, consumer cost savings, and reduced CO 2 emissions atmore » the state level and at aggregated levels. This analysis does not represent all potential savings from energy codes in the U.S. because it excludes several states which have codes which are fundamentally different from the national model energy codes or which do not have state-wide codes. Energy codes follow a three-phase cycle that starts with the development of a new model code, proceeds with the adoption of the new code by states and local jurisdictions, and finishes when buildings comply with the code. The development of new model code editions creates the potential for increased energy savings. After a new model code is adopted, potential savings are realized in the field when new buildings (or additions and alterations) are constructed to comply with the new code. Delayed adoption of a model code and incomplete compliance with the code’s requirements erode potential savings. The contributions of all three phases are crucial to the overall impact of codes, and are considered in this assessment.« less

Multidimensional Trellis Coded Phase Modulation Using a Multilevel Concatenation Approach. Part 1; Code Design

NASA Technical Reports Server (NTRS)

Rajpal, Sandeep; Rhee, Do Jun; Lin, Shu

1997-01-01

The first part of this paper presents a simple and systematic technique for constructing multidimensional M-ary phase shift keying (MMK) trellis coded modulation (TCM) codes. The construction is based on a multilevel concatenation approach in which binary convolutional codes with good free branch distances are used as the outer codes and block MPSK modulation codes are used as the inner codes (or the signal spaces). Conditions on phase invariance of these codes are derived and a multistage decoding scheme for these codes is proposed. The proposed technique can be used to construct good codes for both the additive white Gaussian noise (AWGN) and fading channels as is shown in the second part of this paper.

User's manual for Axisymmetric Diffuser Duct (ADD) code. Volume 1: General ADD code description

NASA Technical Reports Server (NTRS)

Anderson, O. L.; Hankins, G. B., Jr.; Edwards, D. E.

1982-01-01

This User's Manual contains a complete description of the computer codes known as the AXISYMMETRIC DIFFUSER DUCT code or ADD code. It includes a list of references which describe the formulation of the ADD code and comparisons of calculation with experimental flows. The input/output and general use of the code is described in the first volume. The second volume contains a detailed description of the code including the global structure of the code, list of FORTRAN variables, and descriptions of the subroutines. The third volume contains a detailed description of the CODUCT code which generates coordinate systems for arbitrary axisymmetric ducts.

Performance of automated and manual coding systems for occupational data: a case study of historical records.

PubMed

Patel, Mehul D; Rose, Kathryn M; Owens, Cindy R; Bang, Heejung; Kaufman, Jay S

2012-03-01

Occupational data are a common source of workplace exposure and socioeconomic information in epidemiologic research. We compared the performance of two occupation coding methods, an automated software and a manual coder, using occupation and industry titles from U.S. historical records. We collected parental occupational data from 1920-40s birth certificates, Census records, and city directories on 3,135 deceased individuals in the Atherosclerosis Risk in Communities (ARIC) study. Unique occupation-industry narratives were assigned codes by a manual coder and the Standardized Occupation and Industry Coding software program. We calculated agreement between coding methods of classification into major Census occupational groups. Automated coding software assigned codes to 71% of occupations and 76% of industries. Of this subset coded by software, 73% of occupation codes and 69% of industry codes matched between automated and manual coding. For major occupational groups, agreement improved to 89% (kappa = 0.86). Automated occupational coding is a cost-efficient alternative to manual coding. However, some manual coding is required to code incomplete information. We found substantial variability between coders in the assignment of occupations although not as large for major groups.

Convolutional coding techniques for data protection

NASA Technical Reports Server (NTRS)

Massey, J. L.

1975-01-01

Results of research on the use of convolutional codes in data communications are presented. Convolutional coding fundamentals are discussed along with modulation and coding interaction. Concatenated coding systems and data compression with convolutional codes are described.

Alternative Fuels Data Center: Codes and Standards Resources

Science.gov Websites

codes and standards. Biodiesel Vehicle and Infrastructure Codes and Standards Chart Electric Vehicle and Infrastructure Codes and Standards Chart Ethanol Vehicle and Infrastructure Codes and Standards Chart Natural Gas Vehicle and Infrastructure Codes and Standards Chart Propane Vehicle and Infrastructure Codes and

The study on dynamic cadastral coding rules based on kinship relationship

NASA Astrophysics Data System (ADS)

Xu, Huan; Liu, Nan; Liu, Renyi; Lu, Jingfeng

2007-06-01

Cadastral coding rules are an important supplement to the existing national and local standard specifications for building cadastral database. After analyzing the course of cadastral change, especially the parcel change with the method of object-oriented analysis, a set of dynamic cadastral coding rules based on kinship relationship corresponding to the cadastral change is put forward and a coding format composed of street code, block code, father parcel code, child parcel code and grandchild parcel code is worked out within the county administrative area. The coding rule has been applied to the development of an urban cadastral information system called "ReGIS", which is not only able to figure out the cadastral code automatically according to both the type of parcel change and the coding rules, but also capable of checking out whether the code is spatiotemporally unique before the parcel is stored in the database. The system has been used in several cities of Zhejiang Province and got a favorable response. This verifies the feasibility and effectiveness of the coding rules to some extent.

A low-complexity and high performance concatenated coding scheme for high-speed satellite communications

NASA Technical Reports Server (NTRS)

Lin, Shu; Rhee, Dojun; Rajpal, Sandeep

1993-01-01

This report presents a low-complexity and high performance concatenated coding scheme for high-speed satellite communications. In this proposed scheme, the NASA Standard Reed-Solomon (RS) code over GF(2(exp 8) is used as the outer code and the second-order Reed-Muller (RM) code of Hamming distance 8 is used as the inner code. The RM inner code has a very simple trellis structure and is decoded with the soft-decision Viterbi decoding algorithm. It is shown that the proposed concatenated coding scheme achieves an error performance which is comparable to that of the NASA TDRS concatenated coding scheme in which the NASA Standard rate-1/2 convolutional code of constraint length 7 and d sub free = 10 is used as the inner code. However, the proposed RM inner code has much smaller decoding complexity, less decoding delay, and much higher decoding speed. Consequently, the proposed concatenated coding scheme is suitable for reliable high-speed satellite communications, and it may be considered as an alternate coding scheme for the NASA TDRS system.

Construction of self-dual codes in the Rosenbloom-Tsfasman metric

NASA Astrophysics Data System (ADS)

Krisnawati, Vira Hari; Nisa, Anzi Lina Ukhtin

2017-12-01

Linear code is a very basic code and very useful in coding theory. Generally, linear code is a code over finite field in Hamming metric. Among the most interesting families of codes, the family of self-dual code is a very important one, because it is the best known error-correcting code. The concept of Hamming metric is develop into Rosenbloom-Tsfasman metric (RT-metric). The inner product in RT-metric is different from Euclid inner product that is used to define duality in Hamming metric. Most of the codes which are self-dual in Hamming metric are not so in RT-metric. And, generator matrix is very important to construct a code because it contains basis of the code. Therefore in this paper, we give some theorems and methods to construct self-dual codes in RT-metric by considering properties of the inner product and generator matrix. Also, we illustrate some examples for every kind of the construction.

Two Perspectives on the Origin of the Standard Genetic Code

NASA Astrophysics Data System (ADS)

Sengupta, Supratim; Aggarwal, Neha; Bandhu, Ashutosh Vishwa

2014-12-01

The origin of a genetic code made it possible to create ordered sequences of amino acids. In this article we provide two perspectives on code origin by carrying out simulations of code-sequence coevolution in finite populations with the aim of examining how the standard genetic code may have evolved from more primitive code(s) encoding a small number of amino acids. We determine the efficacy of the physico-chemical hypothesis of code origin in the absence and presence of horizontal gene transfer (HGT) by allowing a diverse collection of code-sequence sets to compete with each other. We find that in the absence of horizontal gene transfer, natural selection between competing codes distinguished by differences in the degree of physico-chemical optimization is unable to explain the structure of the standard genetic code. However, for certain probabilities of the horizontal transfer events, a universal code emerges having a structure that is consistent with the standard genetic code.

Development of new two-dimensional spectral/spatial code based on dynamic cyclic shift code for OCDMA system

NASA Astrophysics Data System (ADS)

Jellali, Nabiha; Najjar, Monia; Ferchichi, Moez; Rezig, Houria

2017-07-01

In this paper, a new two-dimensional spectral/spatial codes family, named two dimensional dynamic cyclic shift codes (2D-DCS) is introduced. The 2D-DCS codes are derived from the dynamic cyclic shift code for the spectral and spatial coding. The proposed system can fully eliminate the multiple access interference (MAI) by using the MAI cancellation property. The effect of shot noise, phase-induced intensity noise and thermal noise are used to analyze the code performance. In comparison with existing two dimensional (2D) codes, such as 2D perfect difference (2D-PD), 2D Extended Enhanced Double Weight (2D-Extended-EDW) and 2D hybrid (2D-FCC/MDW) codes, the numerical results show that our proposed codes have the best performance. By keeping the same code length and increasing the spatial code, the performance of our 2D-DCS system is enhanced: it provides higher data rates while using lower transmitted power and a smaller spectral width.

Quality improvement of International Classification of Diseases, 9th revision, diagnosis coding in radiation oncology: single-institution prospective study at University of California, San Francisco.

PubMed

Chen, Chien P; Braunstein, Steve; Mourad, Michelle; Hsu, I-Chow J; Haas-Kogan, Daphne; Roach, Mack; Fogh, Shannon E

2015-01-01

Accurate International Classification of Diseases (ICD) diagnosis coding is critical for patient care, billing purposes, and research endeavors. In this single-institution study, we evaluated our baseline ICD-9 (9th revision) diagnosis coding accuracy, identified the most common errors contributing to inaccurate coding, and implemented a multimodality strategy to improve radiation oncology coding. We prospectively studied ICD-9 coding accuracy in our radiation therapy--specific electronic medical record system. Baseline ICD-9 coding accuracy was obtained from chart review targeting ICD-9 coding accuracy of all patients treated at our institution between March and June of 2010. To improve performance an educational session highlighted common coding errors, and a user-friendly software tool, RadOnc ICD Search, version 1.0, for coding radiation oncology specific diagnoses was implemented. We then prospectively analyzed ICD-9 coding accuracy for all patients treated from July 2010 to June 2011, with the goal of maintaining 80% or higher coding accuracy. Data on coding accuracy were analyzed and fed back monthly to individual providers. Baseline coding accuracy for physicians was 463 of 661 (70%) cases. Only 46% of physicians had coding accuracy above 80%. The most common errors involved metastatic cases, whereby primary or secondary site ICD-9 codes were either incorrect or missing, and special procedures such as stereotactic radiosurgery cases. After implementing our project, overall coding accuracy rose to 92% (range, 86%-96%). The median accuracy for all physicians was 93% (range, 77%-100%) with only 1 attending having accuracy below 80%. Incorrect primary and secondary ICD-9 codes in metastatic cases showed the most significant improvement (10% vs 2% after intervention). Identifying common coding errors and implementing both education and systems changes led to significantly improved coding accuracy. This quality assurance project highlights the potential problem of ICD-9 coding accuracy by physicians and offers an approach to effectively address this shortcoming. Copyright © 2015. Published by Elsevier Inc.

Spread Spectrum Visual Sensor Network Resource Management Using an End-to-End Cross-Layer Design

DTIC Science & Technology

2011-02-01

Coding In this work, we use rate compatible punctured convolutional (RCPC) codes for channel coding [11]. Using RCPC codes al- lows us to utilize Viterbi’s...11] J. Hagenauer, “ Rate - compatible punctured convolutional codes (RCPC codes ) and their applications,” IEEE Trans. Commun., vol. 36, no. 4, pp. 389...source coding rate , a channel coding rate , and a power level to all nodes in the

New quantum codes derived from a family of antiprimitive BCH codes

NASA Astrophysics Data System (ADS)

Liu, Yang; Li, Ruihu; Lü, Liangdong; Guo, Luobin

The Bose-Chaudhuri-Hocquenghem (BCH) codes have been studied for more than 57 years and have found wide application in classical communication system and quantum information theory. In this paper, we study the construction of quantum codes from a family of q2-ary BCH codes with length n=q2m+1 (also called antiprimitive BCH codes in the literature), where q≥4 is a power of 2 and m≥2. By a detailed analysis of some useful properties about q2-ary cyclotomic cosets modulo n, Hermitian dual-containing conditions for a family of non-narrow-sense antiprimitive BCH codes are presented, which are similar to those of q2-ary primitive BCH codes. Consequently, via Hermitian Construction, a family of new quantum codes can be derived from these dual-containing BCH codes. Some of these new antiprimitive quantum BCH codes are comparable with those derived from primitive BCH codes.

Constructions for finite-state codes

NASA Technical Reports Server (NTRS)

Pollara, F.; Mceliece, R. J.; Abdel-Ghaffar, K.

1987-01-01

A class of codes called finite-state (FS) codes is defined and investigated. These codes, which generalize both block and convolutional codes, are defined by their encoders, which are finite-state machines with parallel inputs and outputs. A family of upper bounds on the free distance of a given FS code is derived from known upper bounds on the minimum distance of block codes. A general construction for FS codes is then given, based on the idea of partitioning a given linear block into cosets of one of its subcodes, and it is shown that in many cases the FS codes constructed in this way have a d sub free which is as large as possible. These codes are found without the need for lengthy computer searches, and have potential applications for future deep-space coding systems. The issue of catastropic error propagation (CEP) for FS codes is also investigated.

Enhanced 2/3 four-ary modulation code using soft-decision Viterbi decoding for four-level holographic data storage systems

NASA Astrophysics Data System (ADS)

Kong, Gyuyeol; Choi, Sooyong

2017-09-01

An enhanced 2/3 four-ary modulation code using soft-decision Viterbi decoding is proposed for four-level holographic data storage systems. While the previous four-ary modulation codes focus on preventing maximum two-dimensional intersymbol interference patterns, the proposed four-ary modulation code aims at maximizing the coding gains for better bit error rate performances. For achieving significant coding gains from the four-ary modulation codes, we design a new 2/3 four-ary modulation code in order to enlarge the free distance on the trellis through extensive simulation. The free distance of the proposed four-ary modulation code is extended from 1.21 to 2.04 compared with that of the conventional four-ary modulation code. The simulation result shows that the proposed four-ary modulation code has more than 1 dB gains compared with the conventional four-ary modulation code.

A multidisciplinary approach to vascular surgery procedure coding improves coding accuracy, work relative value unit assignment, and reimbursement.

PubMed

Aiello, Francesco A; Judelson, Dejah R; Messina, Louis M; Indes, Jeffrey; FitzGerald, Gordon; Doucet, Danielle R; Simons, Jessica P; Schanzer, Andres

2016-08-01

Vascular surgery procedural reimbursement depends on accurate procedural coding and documentation. Despite the critical importance of correct coding, there has been a paucity of research focused on the effect of direct physician involvement. We hypothesize that direct physician involvement in procedural coding will lead to improved coding accuracy, increased work relative value unit (wRVU) assignment, and increased physician reimbursement. This prospective observational cohort study evaluated procedural coding accuracy of fistulograms at an academic medical institution (January-June 2014). All fistulograms were coded by institutional coders (traditional coding) and by a single vascular surgeon whose codes were verified by two institution coders (multidisciplinary coding). The coding methods were compared, and differences were translated into revenue and wRVUs using the Medicare Physician Fee Schedule. Comparison between traditional and multidisciplinary coding was performed for three discrete study periods: baseline (period 1), after a coding education session for physicians and coders (period 2), and after a coding education session with implementation of an operative dictation template (period 3). The accuracy of surgeon operative dictations during each study period was also assessed. An external validation at a second academic institution was performed during period 1 to assess and compare coding accuracy. During period 1, traditional coding resulted in a 4.4% (P = .004) loss in reimbursement and a 5.4% (P = .01) loss in wRVUs compared with multidisciplinary coding. During period 2, no significant difference was found between traditional and multidisciplinary coding in reimbursement (1.3% loss; P = .24) or wRVUs (1.8% loss; P = .20). During period 3, traditional coding yielded a higher overall reimbursement (1.3% gain; P = .26) than multidisciplinary coding. This increase, however, was due to errors by institution coders, with six inappropriately used codes resulting in a higher overall reimbursement that was subsequently corrected. Assessment of physician documentation showed improvement, with decreased documentation errors at each period (11% vs 3.1% vs 0.6%; P = .02). Overall, between period 1 and period 3, multidisciplinary coding resulted in a significant increase in additional reimbursement ($17.63 per procedure; P = .004) and wRVUs (0.50 per procedure; P = .01). External validation at a second academic institution was performed to assess coding accuracy during period 1. Similar to institution 1, traditional coding revealed an 11% loss in reimbursement ($13,178 vs $14,630; P = .007) and a 12% loss in wRVU (293 vs 329; P = .01) compared with multidisciplinary coding. Physician involvement in the coding of endovascular procedures leads to improved procedural coding accuracy, increased wRVU assignments, and increased physician reimbursement. Copyright © 2016 Society for Vascular Surgery. Published by Elsevier Inc. All rights reserved.

Manual versus automated coding of free-text self-reported medication data in the 45 and Up Study: a validation study.

PubMed

Gnjidic, Danijela; Pearson, Sallie-Anne; Hilmer, Sarah N; Basilakis, Jim; Schaffer, Andrea L; Blyth, Fiona M; Banks, Emily

2015-03-30

Increasingly, automated methods are being used to code free-text medication data, but evidence on the validity of these methods is limited. To examine the accuracy of automated coding of previously keyed in free-text medication data compared with manual coding of original handwritten free-text responses (the 'gold standard'). A random sample of 500 participants (475 with and 25 without medication data in the free-text box) enrolled in the 45 and Up Study was selected. Manual coding involved medication experts keying in free-text responses and coding using Anatomical Therapeutic Chemical (ATC) codes (i.e. chemical substance 7-digit level; chemical subgroup 5-digit; pharmacological subgroup 4-digit; therapeutic subgroup 3-digit). Using keyed-in free-text responses entered by non-experts, the automated approach coded entries using the Australian Medicines Terminology database and assigned corresponding ATC codes. Based on manual coding, 1377 free-text entries were recorded and, of these, 1282 medications were coded to ATCs manually. The sensitivity of automated coding compared with manual coding was 79% (n = 1014) for entries coded at the exact ATC level, and 81.6% (n = 1046), 83.0% (n = 1064) and 83.8% (n = 1074) at the 5, 4 and 3-digit ATC levels, respectively. The sensitivity of automated coding for blank responses was 100% compared with manual coding. Sensitivity of automated coding was highest for prescription medications and lowest for vitamins and supplements, compared with the manual approach. Positive predictive values for automated coding were above 95% for 34 of the 38 individual prescription medications examined. Automated coding for free-text prescription medication data shows very high to excellent sensitivity and positive predictive values, indicating that automated methods can potentially be useful for large-scale, medication-related research.

Investigation of Near Shannon Limit Coding Schemes

NASA Technical Reports Server (NTRS)

Kwatra, S. C.; Kim, J.; Mo, Fan

1999-01-01

Turbo codes can deliver performance that is very close to the Shannon limit. This report investigates algorithms for convolutional turbo codes and block turbo codes. Both coding schemes can achieve performance near Shannon limit. The performance of the schemes is obtained using computer simulations. There are three sections in this report. First section is the introduction. The fundamental knowledge about coding, block coding and convolutional coding is discussed. In the second section, the basic concepts of convolutional turbo codes are introduced and the performance of turbo codes, especially high rate turbo codes, is provided from the simulation results. After introducing all the parameters that help turbo codes achieve such a good performance, it is concluded that output weight distribution should be the main consideration in designing turbo codes. Based on the output weight distribution, the performance bounds for turbo codes are given. Then, the relationships between the output weight distribution and the factors like generator polynomial, interleaver and puncturing pattern are examined. The criterion for the best selection of system components is provided. The puncturing pattern algorithm is discussed in detail. Different puncturing patterns are compared for each high rate. For most of the high rate codes, the puncturing pattern does not show any significant effect on the code performance if pseudo - random interleaver is used in the system. For some special rate codes with poor performance, an alternative puncturing algorithm is designed which restores their performance close to the Shannon limit. Finally, in section three, for iterative decoding of block codes, the method of building trellis for block codes, the structure of the iterative decoding system and the calculation of extrinsic values are discussed.

Audit of Clinical Coding of Major Head and Neck Operations

PubMed Central

Mitra, Indu; Malik, Tass; Homer, Jarrod J; Loughran, Sean

2009-01-01

INTRODUCTION Within the NHS, operations are coded using the Office of Population Censuses and Surveys (OPCS) classification system. These codes, together with diagnostic codes, are used to generate Healthcare Resource Group (HRG) codes, which correlate to a payment bracket. The aim of this study was to determine whether allocated procedure codes for major head and neck operations were correct and reflective of the work undertaken. HRG codes generated were assessed to determine accuracy of remuneration. PATIENTS AND METHODS The coding of consecutive major head and neck operations undertaken in a tertiary referral centre over a retrospective 3-month period were assessed. Procedure codes were initially ascribed by professional hospital coders. Operations were then recoded by the surgical trainee in liaison with the head of clinical coding. The initial and revised procedure codes were compared and used to generate HRG codes, to determine whether the payment banding had altered. RESULTS A total of 34 cases were reviewed. The number of procedure codes generated initially by the clinical coders was 99, whereas the revised codes generated 146. Of the original codes, 47 of 99 (47.4%) were incorrect. In 19 of the 34 cases reviewed (55.9%), the HRG code remained unchanged, thus resulting in the correct payment. Six cases were never coded, equating to £15,300 loss of payment. CONCLUSIONS These results highlight the inadequacy of this system to reward hospitals for the work carried out within the NHS in a fair and consistent manner. The current coding system was found to be complicated, ambiguous and inaccurate, resulting in loss of remuneration. PMID:19220944

Reviewing the Challenges and Opportunities Presented by Code Switching and Mixing in Bangla

ERIC Educational Resources Information Center

Hasan, Md. Kamrul; Akhand, Mohd. Moniruzzaman

2015-01-01

This paper investigates the issues related to code-switching/code-mixing in an ESL context. Some preliminary data on Bangla-English code-switching/code-mixing has been analyzed in order to determine which structural pattern of code-switching/code-mixing is predominant in different social strata. This study also explores the relationship of…

Reviewing the Challenges and Opportunities Presented by Code Switching and Mixing in Bangla

ERIC Educational Resources Information Center

Hasan, Md. Kamrul; Akhand, Mohd. Moniruzzaman

2014-01-01

This paper investigates the issues related to code-switching/code-mixing in an ESL context. Some preliminary data on Bangla-English code-switching/code-mixing has been analyzed in order to determine which structural pattern of code-switching/code-mixing is predominant in different social strata. This study also explores the relationship of…

Number of minimum-weight code words in a product code

NASA Technical Reports Server (NTRS)

Miller, R. L.

1978-01-01

Consideration is given to the number of minimum-weight code words in a product code. The code is considered as a tensor product of linear codes over a finite field. Complete theorems and proofs are presented.

National Underground Mines Inventory

DTIC Science & Technology

1983-10-01

system is well designed to minimize water accumulation on the drift levels. In many areas, sufficient water has accumulated to make the use of boots a...four characters designate Field office. 17-18 State Code Pic 99 FIPS code for state in which minets located. 19-21 County Code Plc 999 FIPS code for... Designate a general product class based onSIC code. 28-29 Nine Type Plc 99 Natal/Nonmetal mine type code. Based on subunit operations code and canvass code

Efficient Signal, Code, and Receiver Designs for MIMO Communication Systems

DTIC Science & Technology

2003-06-01

167 5-31 Concatenation of a tilted-QAM inner code with an LDPC outer code with a two component iterative soft-decision decoder. . . . . . . . . 168 5...for AWGN channels has long been studied. There are well-known soft-decision codes like the turbo codes and LDPC codes that can approach capacity to...bits) low density parity check ( LDPC ) code 1. 2. The coded bits are randomly interleaved so that bits nearby go through different sub-channels, and are

Measuring diagnoses: ICD code accuracy.

PubMed

O'Malley, Kimberly J; Cook, Karon F; Price, Matt D; Wildes, Kimberly Raiford; Hurdle, John F; Ashton, Carol M

2005-10-01

To examine potential sources of errors at each step of the described inpatient International Classification of Diseases (ICD) coding process. The use of disease codes from the ICD has expanded from classifying morbidity and mortality information for statistical purposes to diverse sets of applications in research, health care policy, and health care finance. By describing a brief history of ICD coding, detailing the process for assigning codes, identifying where errors can be introduced into the process, and reviewing methods for examining code accuracy, we help code users more systematically evaluate code accuracy for their particular applications. We summarize the inpatient ICD diagnostic coding process from patient admission to diagnostic code assignment. We examine potential sources of errors at each step and offer code users a tool for systematically evaluating code accuracy. Main error sources along the "patient trajectory" include amount and quality of information at admission, communication among patients and providers, the clinician's knowledge and experience with the illness, and the clinician's attention to detail. Main error sources along the "paper trail" include variance in the electronic and written records, coder training and experience, facility quality-control efforts, and unintentional and intentional coder errors, such as misspecification, unbundling, and upcoding. By clearly specifying the code assignment process and heightening their awareness of potential error sources, code users can better evaluate the applicability and limitations of codes for their particular situations. ICD codes can then be used in the most appropriate ways.

Towers of generalized divisible quantum codes

NASA Astrophysics Data System (ADS)

Haah, Jeongwan

2018-04-01

A divisible binary classical code is one in which every code word has weight divisible by a fixed integer. If the divisor is 2ν for a positive integer ν , then one can construct a Calderbank-Shor-Steane (CSS) code, where X -stabilizer space is the divisible classical code, that admits a transversal gate in the ν th level of Clifford hierarchy. We consider a generalization of the divisibility by allowing a coefficient vector of odd integers with which every code word has zero dot product modulo the divisor. In this generalized sense, we construct a CSS code with divisor 2ν +1 and code distance d from any CSS code of code distance d and divisor 2ν where the transversal X is a nontrivial logical operator. The encoding rate of the new code is approximately d times smaller than that of the old code. In particular, for large d and ν ≥2 , our construction yields a CSS code of parameters [[O (dν -1) ,Ω (d ) ,d ] ] admitting a transversal gate at the ν th level of Clifford hierarchy. For our construction we introduce a conversion from magic state distillation protocols based on Clifford measurements to those based on codes with transversal T gates. Our tower contains, as a subclass, generalized triply even CSS codes that have appeared in so-called gauge fixing or code switching methods.

Coordinated design of coding and modulation systems

NASA Technical Reports Server (NTRS)

Massey, J. L.; Ancheta, T.; Johannesson, R.; Lauer, G.; Lee, L.

1976-01-01

The joint optimization of the coding and modulation systems employed in telemetry systems was investigated. Emphasis was placed on formulating inner and outer coding standards used by the Goddard Spaceflight Center. Convolutional codes were found that are nearly optimum for use with Viterbi decoding in the inner coding of concatenated coding systems. A convolutional code, the unit-memory code, was discovered and is ideal for inner system usage because of its byte-oriented structure. Simulations of sequential decoding on the deep-space channel were carried out to compare directly various convolutional codes that are proposed for use in deep-space systems.

A new code for Galileo

NASA Technical Reports Server (NTRS)

Dolinar, S.

1988-01-01

Over the past six to eight years, an extensive research effort was conducted to investigate advanced coding techniques which promised to yield more coding gain than is available with current NASA standard codes. The delay in Galileo's launch due to the temporary suspension of the shuttle program provided the Galileo project with an opportunity to evaluate the possibility of including some version of the advanced codes as a mission enhancement option. A study was initiated last summer to determine if substantial coding gain was feasible for Galileo and, is so, to recommend a suitable experimental code for use as a switchable alternative to the current NASA-standard code. The Galileo experimental code study resulted in the selection of a code with constant length 15 and rate 1/4. The code parameters were chosen to optimize performance within cost and risk constraints consistent with retrofitting the new code into the existing Galileo system design and launch schedule. The particular code was recommended after a very limited search among good codes with the chosen parameters. It will theoretically yield about 1.5 dB enhancement under idealizing assumptions relative to the current NASA-standard code at Galileo's desired bit error rates. This ideal predicted gain includes enough cushion to meet the project's target of at least 1 dB enhancement under real, non-ideal conditions.

The Mystery Behind the Code: Differentiated Instruction with Quick Response Codes in Secondary Physical Education

ERIC Educational Resources Information Center

Adkins, Megan; Wajciechowski, Misti R.; Scantling, Ed

2013-01-01

Quick response codes, better known as QR codes, are small barcodes scanned to receive information about a specific topic. This article explains QR code technology and the utility of QR codes in the delivery of physical education instruction. Consideration is given to how QR codes can be used to accommodate learners of varying ability levels as…

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR CODING AND CODING VERIFICATION (HAND ENTRY) (UA-D-14.0)

EPA Science Inventory

The purpose of this SOP is to define the coding strategy for coding and coding verification of hand-entered data. It applies to the coding of all physical forms, especially those coded by hand. The strategy was developed for use in the Arizona NHEXAS project and the "Border" st...

Hyperbolic and semi-hyperbolic surface codes for quantum storage

NASA Astrophysics Data System (ADS)

Breuckmann, Nikolas P.; Vuillot, Christophe; Campbell, Earl; Krishna, Anirudh; Terhal, Barbara M.

2017-09-01

We show how a hyperbolic surface code could be used for overhead-efficient quantum storage. We give numerical evidence for a noise threshold of 1.3 % for the \\{4,5\\}-hyperbolic surface code in a phenomenological noise model (as compared with 2.9 % for the toric code). In this code family, parity checks are of weight 4 and 5, while each qubit participates in four different parity checks. We introduce a family of semi-hyperbolic codes that interpolate between the toric code and the \\{4,5\\}-hyperbolic surface code in terms of encoding rate and threshold. We show how these hyperbolic codes outperform the toric code in terms of qubit overhead for a target logical error probability. We show how Dehn twists and lattice code surgery can be used to read and write individual qubits to this quantum storage medium.

Convolutional coding combined with continuous phase modulation

NASA Technical Reports Server (NTRS)

Pizzi, S. V.; Wilson, S. G.

1985-01-01

Background theory and specific coding designs for combined coding/modulation schemes utilizing convolutional codes and continuous-phase modulation (CPM) are presented. In this paper the case of r = 1/2 coding onto a 4-ary CPM is emphasized, with short-constraint length codes presented for continuous-phase FSK, double-raised-cosine, and triple-raised-cosine modulation. Coding buys several decibels of coding gain over the Gaussian channel, with an attendant increase of bandwidth. Performance comparisons in the power-bandwidth tradeoff with other approaches are made.

Accumulate Repeat Accumulate Coded Modulation

NASA Technical Reports Server (NTRS)

Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

2004-01-01

In this paper we propose an innovative coded modulation scheme called 'Accumulate Repeat Accumulate Coded Modulation' (ARA coded modulation). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes that are combined with high level modulation. Thus at the decoder belief propagation can be used for iterative decoding of ARA coded modulation on a graph, provided a demapper transforms the received in-phase and quadrature samples to reliability of the bits.

PNPN Latchup in Bipolar LSI Devices.

DTIC Science & Technology

1982-01-01

6611, J. Ritter ATTN: STEWS -TE-N, T. Arellanes ATTN: Code 6612, G. McLane ATTN: STEWS -TE-AN, R. Dutchover ATTN: Code 6816, H. Hughes ATTN: STEWS -TE...AN, R. Hays ATTN: Code 6653, A. Namenson * ATTN: STEWS -TE-N, K. Cummings ATTN: Code 6611, L. August ATTN: STEWS -TE-NT, M. Squires ATTN: Code 6813, J...Killiany ATTN: STEWS -TE-AN, J. Meason ATTN: Code 6600, J. Schriempf ATTN: STEWS -TE-AN, A. De La Paz ATTN: Code 6610, R. Marlow USA MATTN: Code 2627USA

Multi-level trellis coded modulation and multi-stage decoding

NASA Technical Reports Server (NTRS)

Costello, Daniel J., Jr.; Wu, Jiantian; Lin, Shu

1990-01-01

Several constructions for multi-level trellis codes are presented and many codes with better performance than previously known codes are found. These codes provide a flexible trade-off between coding gain, decoding complexity, and decoding delay. New multi-level trellis coded modulation schemes using generalized set partitioning methods are developed for Quadrature Amplitude Modulation (QAM) and Phase Shift Keying (PSK) signal sets. New rotationally invariant multi-level trellis codes which can be combined with differential encoding to resolve phase ambiguity are presented.

What to do with a Dead Research Code

NASA Astrophysics Data System (ADS)

Nemiroff, Robert J.

2016-01-01

The project has ended -- should all of the computer codes that enabled the project be deleted? No. Like research papers, research codes typically carry valuable information past project end dates. Several possible end states to the life of research codes are reviewed. Historically, codes are typically left dormant on an increasingly obscure local disk directory until forgotten. These codes will likely become any or all of: lost, impossible to compile and run, difficult to decipher, and likely deleted when the code's proprietor moves on or dies. It is argued here, though, that it would be better for both code authors and astronomy generally if project codes were archived after use in some way. Archiving is advantageous for code authors because archived codes might increase the author's ADS citable publications, while astronomy as a science gains transparency and reproducibility. Paper-specific codes should be included in the publication of the journal papers they support, just like figures and tables. General codes that support multiple papers, possibly written by multiple authors, including their supporting websites, should be registered with a code registry such as the Astrophysics Source Code Library (ASCL). Codes developed on GitHub can be archived with a third party service such as, currently, BackHub. An important code version might be uploaded to a web archiving service like, currently, Zenodo or Figshare, so that this version receives a Digital Object Identifier (DOI), enabling it to found at a stable address into the future. Similar archiving services that are not DOI-dependent include perma.cc and the Internet Archive Wayback Machine at archive.org. Perhaps most simply, copies of important codes with lasting value might be kept on a cloud service like, for example, Google Drive, while activating Google's Inactive Account Manager.

Syndrome-source-coding and its universal generalization. [error correcting codes for data compression

NASA Technical Reports Server (NTRS)

Ancheta, T. C., Jr.

1976-01-01

A method of using error-correcting codes to obtain data compression, called syndrome-source-coding, is described in which the source sequence is treated as an error pattern whose syndrome forms the compressed data. It is shown that syndrome-source-coding can achieve arbitrarily small distortion with the number of compressed digits per source digit arbitrarily close to the entropy of a binary memoryless source. A 'universal' generalization of syndrome-source-coding is formulated which provides robustly effective distortionless coding of source ensembles. Two examples are given, comparing the performance of noiseless universal syndrome-source-coding to (1) run-length coding and (2) Lynch-Davisson-Schalkwijk-Cover universal coding for an ensemble of binary memoryless sources.

The optimal code searching method with an improved criterion of coded exposure for remote sensing image restoration

NASA Astrophysics Data System (ADS)

He, Lirong; Cui, Guangmang; Feng, Huajun; Xu, Zhihai; Li, Qi; Chen, Yueting

2015-03-01

Coded exposure photography makes the motion de-blurring a well-posed problem. The integration pattern of light is modulated using the method of coded exposure by opening and closing the shutter within the exposure time, changing the traditional shutter frequency spectrum into a wider frequency band in order to preserve more image information in frequency domain. The searching method of optimal code is significant for coded exposure. In this paper, an improved criterion of the optimal code searching is proposed by analyzing relationship between code length and the number of ones in the code, considering the noise effect on code selection with the affine noise model. Then the optimal code is obtained utilizing the method of genetic searching algorithm based on the proposed selection criterion. Experimental results show that the time consuming of searching optimal code decreases with the presented method. The restoration image is obtained with better subjective experience and superior objective evaluation values.

Evaluation of the efficiency and fault density of software generated by code generators

NASA Technical Reports Server (NTRS)

Schreur, Barbara

1993-01-01

Flight computers and flight software are used for GN&C (guidance, navigation, and control), engine controllers, and avionics during missions. The software development requires the generation of a considerable amount of code. The engineers who generate the code make mistakes and the generation of a large body of code with high reliability requires considerable time. Computer-aided software engineering (CASE) tools are available which generates code automatically with inputs through graphical interfaces. These tools are referred to as code generators. In theory, code generators could write highly reliable code quickly and inexpensively. The various code generators offer different levels of reliability checking. Some check only the finished product while some allow checking of individual modules and combined sets of modules as well. Considering NASA's requirement for reliability, an in house manually generated code is needed. Furthermore, automatically generated code is reputed to be as efficient as the best manually generated code when executed. In house verification is warranted.

Simple scheme for encoding and decoding a qubit in unknown state for various topological codes

PubMed Central

Łodyga, Justyna; Mazurek, Paweł; Grudka, Andrzej; Horodecki, Michał

2015-01-01

We present a scheme for encoding and decoding an unknown state for CSS codes, based on syndrome measurements. We illustrate our method by means of Kitaev toric code, defected-lattice code, topological subsystem code and 3D Haah code. The protocol is local whenever in a given code the crossings between the logical operators consist of next neighbour pairs, which holds for the above codes. For subsystem code we also present scheme in a noisy case, where we allow for bit and phase-flip errors on qubits as well as state preparation and syndrome measurement errors. Similar scheme can be built for two other codes. We show that the fidelity of the protected qubit in the noisy scenario in a large code size limit is of , where p is a probability of error on a single qubit per time step. Regarding Haah code we provide noiseless scheme, leaving the noisy case as an open problem. PMID:25754905

Optimal Codes for the Burst Erasure Channel

NASA Technical Reports Server (NTRS)

Hamkins, Jon

2010-01-01

Deep space communications over noisy channels lead to certain packets that are not decodable. These packets leave gaps, or bursts of erasures, in the data stream. Burst erasure correcting codes overcome this problem. These are forward erasure correcting codes that allow one to recover the missing gaps of data. Much of the recent work on this topic concentrated on Low-Density Parity-Check (LDPC) codes. These are more complicated to encode and decode than Single Parity Check (SPC) codes or Reed-Solomon (RS) codes, and so far have not been able to achieve the theoretical limit for burst erasure protection. A block interleaved maximum distance separable (MDS) code (e.g., an SPC or RS code) offers near-optimal burst erasure protection, in the sense that no other scheme of equal total transmission length and code rate could improve the guaranteed correctible burst erasure length by more than one symbol. The optimality does not depend on the length of the code, i.e., a short MDS code block interleaved to a given length would perform as well as a longer MDS code interleaved to the same overall length. As a result, this approach offers lower decoding complexity with better burst erasure protection compared to other recent designs for the burst erasure channel (e.g., LDPC codes). A limitation of the design is its lack of robustness to channels that have impairments other than burst erasures (e.g., additive white Gaussian noise), making its application best suited for correcting data erasures in layers above the physical layer. The efficiency of a burst erasure code is the length of its burst erasure correction capability divided by the theoretical upper limit on this length. The inefficiency is one minus the efficiency. The illustration compares the inefficiency of interleaved RS codes to Quasi-Cyclic (QC) LDPC codes, Euclidean Geometry (EG) LDPC codes, extended Irregular Repeat Accumulate (eIRA) codes, array codes, and random LDPC codes previously proposed for burst erasure protection. As can be seen, the simple interleaved RS codes have substantially lower inefficiency over a wide range of transmission lengths.

Measuring Diagnoses: ICD Code Accuracy

PubMed Central

O'Malley, Kimberly J; Cook, Karon F; Price, Matt D; Wildes, Kimberly Raiford; Hurdle, John F; Ashton, Carol M

2005-01-01

Objective To examine potential sources of errors at each step of the described inpatient International Classification of Diseases (ICD) coding process. Data Sources/Study Setting The use of disease codes from the ICD has expanded from classifying morbidity and mortality information for statistical purposes to diverse sets of applications in research, health care policy, and health care finance. By describing a brief history of ICD coding, detailing the process for assigning codes, identifying where errors can be introduced into the process, and reviewing methods for examining code accuracy, we help code users more systematically evaluate code accuracy for their particular applications. Study Design/Methods We summarize the inpatient ICD diagnostic coding process from patient admission to diagnostic code assignment. We examine potential sources of errors at each step and offer code users a tool for systematically evaluating code accuracy. Principle Findings Main error sources along the “patient trajectory” include amount and quality of information at admission, communication among patients and providers, the clinician's knowledge and experience with the illness, and the clinician's attention to detail. Main error sources along the “paper trail” include variance in the electronic and written records, coder training and experience, facility quality-control efforts, and unintentional and intentional coder errors, such as misspecification, unbundling, and upcoding. Conclusions By clearly specifying the code assignment process and heightening their awareness of potential error sources, code users can better evaluate the applicability and limitations of codes for their particular situations. ICD codes can then be used in the most appropriate ways. PMID:16178999

Two-terminal video coding.

PubMed

Yang, Yang; Stanković, Vladimir; Xiong, Zixiang; Zhao, Wei

2009-03-01

Following recent works on the rate region of the quadratic Gaussian two-terminal source coding problem and limit-approaching code designs, this paper examines multiterminal source coding of two correlated, i.e., stereo, video sequences to save the sum rate over independent coding of both sequences. Two multiterminal video coding schemes are proposed. In the first scheme, the left sequence of the stereo pair is coded by H.264/AVC and used at the joint decoder to facilitate Wyner-Ziv coding of the right video sequence. The first I-frame of the right sequence is successively coded by H.264/AVC Intracoding and Wyner-Ziv coding. An efficient stereo matching algorithm based on loopy belief propagation is then adopted at the decoder to produce pixel-level disparity maps between the corresponding frames of the two decoded video sequences on the fly. Based on the disparity maps, side information for both motion vectors and motion-compensated residual frames of the right sequence are generated at the decoder before Wyner-Ziv encoding. In the second scheme, source splitting is employed on top of classic and Wyner-Ziv coding for compression of both I-frames to allow flexible rate allocation between the two sequences. Experiments with both schemes on stereo video sequences using H.264/AVC, LDPC codes for Slepian-Wolf coding of the motion vectors, and scalar quantization in conjunction with LDPC codes for Wyner-Ziv coding of the residual coefficients give a slightly lower sum rate than separate H.264/AVC coding of both sequences at the same video quality.

Long distance quantum communication with quantum Reed-Solomon codes

NASA Astrophysics Data System (ADS)

Muralidharan, Sreraman; Zou, Chang-Ling; Li, Linshu; Jiang, Liang; Jianggroup Team

We study the construction of quantum Reed Solomon codes from classical Reed Solomon codes and show that they achieve the capacity of quantum erasure channel for multi-level quantum systems. We extend the application of quantum Reed Solomon codes to long distance quantum communication, investigate the local resource overhead needed for the functioning of one-way quantum repeaters with these codes, and numerically identify the parameter regime where these codes perform better than the known quantum polynomial codes and quantum parity codes . Finally, we discuss the implementation of these codes into time-bin photonic states of qubits and qudits respectively, and optimize the performance for one-way quantum repeaters.

Transversal Clifford gates on folded surface codes

DOE PAGES

Moussa, Jonathan E.

2016-10-12

Surface and color codes are two forms of topological quantum error correction in two spatial dimensions with complementary properties. Surface codes have lower-depth error detection circuits and well-developed decoders to interpret and correct errors, while color codes have transversal Clifford gates and better code efficiency in the number of physical qubits needed to achieve a given code distance. A formal equivalence exists between color codes and folded surface codes, but it does not guarantee the transferability of any of these favorable properties. However, the equivalence does imply the existence of constant-depth circuit implementations of logical Clifford gates on folded surfacemore » codes. We achieve and improve this result by constructing two families of folded surface codes with transversal Clifford gates. This construction is presented generally for qudits of any dimension. Lastly, the specific application of these codes to universal quantum computation based on qubit fusion is also discussed.« less

Generalized type II hybrid ARQ scheme using punctured convolutional coding

NASA Astrophysics Data System (ADS)

Kallel, Samir; Haccoun, David

1990-11-01

A method is presented to construct rate-compatible convolutional (RCC) codes from known high-rate punctured convolutional codes, obtained from best-rate 1/2 codes. The construction method is rather simple and straightforward, and still yields good codes. Moreover, low-rate codes can be obtained without any limit on the lowest achievable code rate. Based on the RCC codes, a generalized type-II hybrid ARQ scheme, which combines the benefits of the modified type-II hybrid ARQ strategy of Hagenauer (1988) with the code-combining ARQ strategy of Chase (1985), is proposed and analyzed. With the proposed generalized type-II hybrid ARQ strategy, the throughput increases as the starting coding rate increases, and as the channel degrades, it tends to merge with the throughput of rate 1/2 type-II hybrid ARQ schemes with code combining, thus allowing the system to be flexible and adaptive to channel conditions, even under wide noise variations and severe degradations.

Comparison of space radiation calculations for deterministic and Monte Carlo transport codes

NASA Astrophysics Data System (ADS)

Lin, Zi-Wei; Adams, James; Barghouty, Abdulnasser; Randeniya, Sharmalee; Tripathi, Ram; Watts, John; Yepes, Pablo

For space radiation protection of astronauts or electronic equipments, it is necessary to develop and use accurate radiation transport codes. Radiation transport codes include deterministic codes, such as HZETRN from NASA and UPROP from the Naval Research Laboratory, and Monte Carlo codes such as FLUKA, the Geant4 toolkit and HETC-HEDS. The deterministic codes and Monte Carlo codes complement each other in that deterministic codes are very fast while Monte Carlo codes are more elaborate. Therefore it is important to investigate how well the results of deterministic codes compare with those of Monte Carlo transport codes and where they differ. In this study we evaluate these different codes in their space radiation applications by comparing their output results in the same given space radiation environments, shielding geometry and material. Typical space radiation environments such as the 1977 solar minimum galactic cosmic ray environment are used as the well-defined input, and simple geometries made of aluminum, water and/or polyethylene are used to represent the shielding material. We then compare various outputs of these codes, such as the dose-depth curves and the flux spectra of different fragments and other secondary particles. These comparisons enable us to learn more about the main differences between these space radiation transport codes. At the same time, they help us to learn the qualitative and quantitative features that these transport codes have in common.

Methodology, status and plans for development and assessment of Cathare code

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bestion, D.; Barre, F.; Faydide, B.

1997-07-01

This paper presents the methodology, status and plans for the development, assessment and uncertainty evaluation of the Cathare code. Cathare is a thermalhydraulic code developed by CEA (DRN), IPSN, EDF and FRAMATOME for PWR safety analysis. First, the status of the code development and assessment is presented. The general strategy used for the development and the assessment of the code is presented. Analytical experiments with separate effect tests, and component tests are used for the development and the validation of closure laws. Successive Revisions of constitutive laws are implemented in successive Versions of the code and assessed. System tests ormore » integral tests are used to validate the general consistency of the Revision. Each delivery of a code Version + Revision is fully assessed and documented. A methodology is being developed to determine the uncertainty on all constitutive laws of the code using calculations of many analytical tests and applying the Discrete Adjoint Sensitivity Method (DASM). At last, the plans for the future developments of the code are presented. They concern the optimization of the code performance through parallel computing - the code will be used for real time full scope plant simulators - the coupling with many other codes (neutronic codes, severe accident codes), the application of the code for containment thermalhydraulics. Also, physical improvements are required in the field of low pressure transients and in the modeling for the 3-D model.« less

Accuracy of external cause-of-injury coding in VA polytrauma patient discharge records.

PubMed

Carlson, Kathleen F; Nugent, Sean M; Grill, Joseph; Sayer, Nina A

2010-01-01

Valid and efficient methods of identifying the etiology of treated injuries are critical for characterizing patient populations and developing prevention and rehabilitation strategies. We examined the accuracy of external cause-of-injury codes (E-codes) in Veterans Health Administration (VHA) administrative data for a population of injured patients. Chart notes and E-codes were extracted for 566 patients treated at any one of four VHA Polytrauma Rehabilitation Center sites between 2001 and 2006. Two expert coders, blinded to VHA E-codes, used chart notes to assign "gold standard" E-codes to injured patients. The accuracy of VHA E-coding was examined based on these gold standard E-codes. Only 382 of 517 (74%) injured patients were assigned E-codes in VHA records. Sensitivity of VHA E-codes varied significantly by site (range: 59%-91%, p < 0.001). Sensitivity was highest for combat-related injuries (81%) and lowest for fall-related injuries (60%). Overall specificity of E-codes was high (92%). E-coding accuracy was markedly higher when we restricted analyses to records that had been assigned VHA E-codes. E-codes may not be valid for ascertaining source-of-injury data for all injuries among VHA rehabilitation inpatients at this time. Enhanced training and policies may ensure more widespread, standardized use and accuracy of E-codes for injured veterans treated in the VHA.

78 FR 18321 - International Code Council: The Update Process for the International Codes and Standards

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-26

... for Residential Construction in High Wind Regions. ICC 700: National Green Building Standard The..., coordinated, and necessary to regulate the built environment. Federal agencies frequently use these codes and... International Codes and Standards consist of the following: ICC Codes International Building Code. International...

75 FR 19944 - International Code Council: The Update Process for the International Codes and Standards

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-16

... for Residential Construction in High Wind Areas. ICC 700: National Green Building Standard. The... Codes and Standards that are comprehensive, coordinated, and necessary to regulate the built environment... International Codes and Standards consist of the following: ICC Codes International Building Code. International...

Model Children's Code.

ERIC Educational Resources Information Center

New Mexico Univ., Albuquerque. American Indian Law Center.

The Model Children's Code was developed to provide a legally correct model code that American Indian tribes can use to enact children's codes that fulfill their legal, cultural and economic needs. Code sections cover the court system, jurisdiction, juvenile offender procedures, minor-in-need-of-care, and termination. Almost every Code section is…

Facilitating Internet-Scale Code Retrieval

ERIC Educational Resources Information Center

Bajracharya, Sushil Krishna

2010-01-01

Internet-Scale code retrieval deals with the representation, storage, and access of relevant source code from a large amount of source code available on the Internet. Internet-Scale code retrieval systems support common emerging practices among software developers related to finding and reusing source code. In this dissertation we focus on some…

47 CFR 15.214 - Cordless telephones.

Code of Federal Regulations, 2010 CFR

2010-10-01

... discrete digital codes. Factory-set codes must be continuously varied over at least 256 possible codes as... readily select from among at least 256 possible discrete digital codes. The cordless telephone shall be... fixed code that is continuously varied among at least 256 discrete digital codes as each telephone is...

28 CFR 36.608 - Guidance concerning model codes.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Guidance concerning model codes. 36.608... Codes § 36.608 Guidance concerning model codes. Upon application by an authorized representative of a... relevant model code and issue guidance concerning whether and in what respects the model code is consistent...