Development and validation of an Argentine set of facial expressions of emotion.

PubMed

Vaiman, Marcelo; Wagner, Mónica Anna; Caicedo, Estefanía; Pereno, Germán Leandro

2017-02-01

Pictures of facial expressions of emotion are used in a wide range of experiments. The last decade has seen an increase in the number of studies presenting local sets of emotion stimuli. However, only a few existing sets contain pictures of Latin Americans, despite the growing attention emotion research is receiving in this region. Here we present the development and validation of the Universidad Nacional de Cordoba, Expresiones de Emociones Faciales (UNCEEF), a Facial Action Coding System (FACS)-verified set of pictures of Argentineans expressing the six basic emotions, plus neutral expressions. FACS scores, recognition rates, Hu scores, and discrimination indices are reported. Evidence of convergent validity was obtained using the Pictures of Facial Affect in an Argentine sample. However, recognition accuracy was greater for UNCEEF. The importance of local sets of emotion pictures is discussed.

48 CFR 301.603-72 - FAC-C and HHS SAC certification requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false FAC-C and HHS SAC... Responsibilities 301.603-72 FAC-C and HHS SAC certification requirements. (a) The FAC-C certification program is... thereunder are not required to re-take training courses, but shall follow FAC-C training requirements when...

48 CFR 301.603-72 - FAC-C and HHS SAC certification requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false FAC-C and HHS SAC... Responsibilities 301.603-72 FAC-C and HHS SAC certification requirements. (a) The FAC-C certification program is... thereunder are not required to re-take training courses, but shall follow FAC-C training requirements when...

48 CFR 301.603-72 - FAC-C and HHS SAC certification requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false FAC-C and HHS SAC... Responsibilities 301.603-72 FAC-C and HHS SAC certification requirements. (a) The FAC-C certification program is... thereunder are not required to re-take training courses, but shall follow FAC-C training requirements when...

48 CFR 301.603-72 - FAC-C and HHS SAC certification requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false FAC-C and HHS SAC... Responsibilities 301.603-72 FAC-C and HHS SAC certification requirements. (a) The FAC-C certification program is... thereunder are not required to re-take training courses, but shall follow FAC-C training requirements when...

The two-way relationship between ionospheric outflow and the ring current

DOE Office of Scientific and Technical Information (OSTI.GOV)

Welling, Daniel T.; Jordanova, Vania Koleva; Glocer, Alex

It is now well established that the ionosphere, because it acts as a significant source of plasma, plays a critical role in ring current dynamics. However, because the ring current deposits energy into the ionosphere, the inverse may also be true: the ring current can play a critical role in the dynamics of ionospheric outflow. This study uses a set of coupled, first-principles-based numerical models to test the dependence of ionospheric outflow on ring current-driven region 2 field-aligned currents (FACs). A moderate magnetospheric storm event is modeled with the Space Weather Modeling Framework using a global MHD code (Block Adaptivemore » Tree Solar wind Roe-type Upwind Scheme, BATS-R-US), a polar wind model (Polar Wind Outflow Model), and a bounce-averaged kinetic ring current model (ring current atmosphere interaction model with self-consistent magnetic field, RAM-SCB). Initially, each code is two-way coupled to all others except for RAM-SCB, which receives inputs from the other models but is not allowed to feed back pressure into the MHD model. The simulation is repeated with pressure coupling activated, which drives strong pressure gradients and region 2 FACs in BATS-R-US. It is found that the region 2 FACs increase heavy ion outflow by up to 6 times over the non-coupled results. The additional outflow further energizes the ring current, establishing an ionosphere-magnetosphere mass feedback loop. This study further demonstrates that ionospheric outflow is not merely a plasma source for the magnetosphere but an integral part in the nonlinear ionosphere-magnetosphere-ring current system.« less

The two-way relationship between ionospheric outflow and the ring current

DOE PAGES

Welling, Daniel T.; Jordanova, Vania Koleva; Glocer, Alex; ...

2015-06-01

It is now well established that the ionosphere, because it acts as a significant source of plasma, plays a critical role in ring current dynamics. However, because the ring current deposits energy into the ionosphere, the inverse may also be true: the ring current can play a critical role in the dynamics of ionospheric outflow. This study uses a set of coupled, first-principles-based numerical models to test the dependence of ionospheric outflow on ring current-driven region 2 field-aligned currents (FACs). A moderate magnetospheric storm event is modeled with the Space Weather Modeling Framework using a global MHD code (Block Adaptivemore » Tree Solar wind Roe-type Upwind Scheme, BATS-R-US), a polar wind model (Polar Wind Outflow Model), and a bounce-averaged kinetic ring current model (ring current atmosphere interaction model with self-consistent magnetic field, RAM-SCB). Initially, each code is two-way coupled to all others except for RAM-SCB, which receives inputs from the other models but is not allowed to feed back pressure into the MHD model. The simulation is repeated with pressure coupling activated, which drives strong pressure gradients and region 2 FACs in BATS-R-US. It is found that the region 2 FACs increase heavy ion outflow by up to 6 times over the non-coupled results. The additional outflow further energizes the ring current, establishing an ionosphere-magnetosphere mass feedback loop. This study further demonstrates that ionospheric outflow is not merely a plasma source for the magnetosphere but an integral part in the nonlinear ionosphere-magnetosphere-ring current system.« less

Electron-impact ionization of P-like ions forming Si-like ions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kwon, D.-H.; Savin, D. W., E-mail: hkwon@kaeri.re.kr

2014-03-20

We have calculated electron-impact ionization (EII) for P-like systems from P to Zn{sup 15+} forming Si-like ions. The work was performed using the flexible atomic code (FAC) which is based on a distorted-wave approximation. All 3ℓ → nℓ' (n = 3-35) excitation-autoionization (EA) channels near the 3p direct ionization threshold and 2ℓ → nℓ' (n = 3-10) EA channels at the higher energies are included. Close attention has been paid to the detailed branching ratios. Our calculated total EII cross sections are compared both with previous FAC calculations, which omitted many of these EA channels, and with the available experimentalmore » results. Moreover, for Fe{sup 11+}, we find that part of the remaining discrepancies between our calculations and recent measurements can be accounted for by the inclusion of the resonant excitation double autoionization process. Lastly, at the temperatures where each ion is predicted to peak in abundances in collisional ionization equilibrium, the Maxwellian rate coefficients derived from our calculations differ by 50%-7% from the previous FAC rate coefficients, with the difference decreasing with increasing charge.« less

Observation of a Unipolar Field-aligned Current System Associated With IMF By-triggered Theta Auroras

NASA Astrophysics Data System (ADS)

Hairston, M. R.; Watanabe, M.

2016-12-01

We investigate the existence of a specific field-aligned current (FAC) system predicted by numerical magnetohydrodynamic simulations in a past study. The FAC system is expected to occur when a drifting theta aurora is formed in response to a stepwise transition of interplanetary magnetic field (IMF) By during strongly northward IMF periods. When the IMF By changes from positive to negative, a crossbar forms in the Northern Hemisphere that moves dawnward, while in the Southern Hemisphere the crossbar moves in the opposite direction. The crossbar motion reverses when the IMF By changes from negative to positive. The FAC system appears on the trailing side of the drifting crossbar of the theta aurora as it moves either dawnward or duskward. When the theta aurora is drifting dawnward, the FACs flow into the ionosphere. The FAC polarity reverses when the theta aurora is drifting duskward. Using low-altitude satellite data, we confirmed the real existence of the above model-predicted FAC system.

The interplanetary magnetic field B[sub y] effects on large-scale field-aligned currents near local noon: Contributions from cusp part and noncusp part

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yamauchi, M.; Lundin, R.; Woch, J.

1993-04-01

latitudinals develop a model to account for the effect of the interplanetary magnetic field (IMF) B[sub y] component on the dayside field-aligned currents (FACs). As part of the model the FACs are divided into a [open quotes]cusp part[close quotes] and a [open quotes]noncusp part[close quotes]. The authors then propose that the cusp part FACs shift in the longitudinal direction while the noncusplike part FACs shift in both longitudinal and latitudinal directions in response to the y component of the IMF. If combined, it is observed that the noncusp part FAC is found poleward of the cusp part FAC system whenmore » the y component of the IMF is large. These two FAC systems flow in the same direction. They reinforce one another, creating a strong FAC, termed the DPY-FAC. The model also predicts that the polewardmost part of the DPY-FAC flows on closed field lines, even in regions conventionally occupied by the polar cap. Results of the model are successfully compared with particle and magnetic field data from Viking missions.« less

Field-aligned currents and large-scale magnetospheric electric fields

NASA Technical Reports Server (NTRS)

Dangelo, N.

1979-01-01

The existence of field-aligned currents (FAC) at northern and southern high latitudes was confirmed by a number of observations, most clearly by experiments on the TRIAD and ISIS 2 satellites. The high-latitude FAC system is used to relate what is presently known about the large-scale pattern of high-latitude ionospheric electric fields and their relation to solar wind parameters. Recently a simplified model was presented for polar cap electric fields. The model is of considerable help in visualizing the large-scale features of FAC systems. A summary of the FAC observations is given. The simplified model is used to visualize how the FAC systems are driven by their generators.

Joint Patch and Multi-label Learning for Facial Action Unit Detection

PubMed Central

Zhao, Kaili; Chu, Wen-Sheng; De la Torre, Fernando; Cohn, Jeffrey F.; Zhang, Honggang

2016-01-01

The face is one of the most powerful channel of nonverbal communication. The most commonly used taxonomy to describe facial behaviour is the Facial Action Coding System (FACS). FACS segments the visible effects of facial muscle activation into 30+ action units (AUs). AUs, which may occur alone and in thousands of combinations, can describe nearly all-possible facial expressions. Most existing methods for automatic AU detection treat the problem using one-vs-all classifiers and fail to exploit dependencies among AU and facial features. We introduce joint-patch and multi-label learning (JPML) to address these issues. JPML leverages group sparsity by selecting a sparse subset of facial patches while learning a multi-label classifier. In four of five comparisons on three diverse datasets, CK+, GFT, and BP4D, JPML produced the highest average F1 scores in comparison with state-of-the art. PMID:27382243

A FPGA Implementation of the CAR-FAC Cochlear Model.

PubMed

Xu, Ying; Thakur, Chetan S; Singh, Ram K; Hamilton, Tara Julia; Wang, Runchun M; van Schaik, André

2018-01-01

This paper presents a digital implementation of the Cascade of Asymmetric Resonators with Fast-Acting Compression (CAR-FAC) cochlear model. The CAR part simulates the basilar membrane's (BM) response to sound. The FAC part models the outer hair cell (OHC), the inner hair cell (IHC), and the medial olivocochlear efferent system functions. The FAC feeds back to the CAR by moving the poles and zeros of the CAR resonators automatically. We have implemented a 70-section, 44.1 kHz sampling rate CAR-FAC system on an Altera Cyclone V Field Programmable Gate Array (FPGA) with 18% ALM utilization by using time-multiplexing and pipeline parallelizing techniques and present measurement results here. The fully digital reconfigurable CAR-FAC system is stable, scalable, easy to use, and provides an excellent input stage to more complex machine hearing tasks such as sound localization, sound segregation, speech recognition, and so on.

A FPGA Implementation of the CAR-FAC Cochlear Model

PubMed Central

Xu, Ying; Thakur, Chetan S.; Singh, Ram K.; Hamilton, Tara Julia; Wang, Runchun M.; van Schaik, André

2018-01-01

This paper presents a digital implementation of the Cascade of Asymmetric Resonators with Fast-Acting Compression (CAR-FAC) cochlear model. The CAR part simulates the basilar membrane's (BM) response to sound. The FAC part models the outer hair cell (OHC), the inner hair cell (IHC), and the medial olivocochlear efferent system functions. The FAC feeds back to the CAR by moving the poles and zeros of the CAR resonators automatically. We have implemented a 70-section, 44.1 kHz sampling rate CAR-FAC system on an Altera Cyclone V Field Programmable Gate Array (FPGA) with 18% ALM utilization by using time-multiplexing and pipeline parallelizing techniques and present measurement results here. The fully digital reconfigurable CAR-FAC system is stable, scalable, easy to use, and provides an excellent input stage to more complex machine hearing tasks such as sound localization, sound segregation, speech recognition, and so on. PMID:29692700

Pipe degradation investigations for optimization of flow-accelerated corrosion inspection location selection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chandra, S.; Habicht, P.; Chexal, B.

1995-12-01

A large amount of piping in a typical nuclear power plant is susceptible to Flow-Accelerated Corrosion (FAC) wall thinning to varying degrees. A typical PAC monitoring program includes the wall thickness measurement of a select number of components in order to judge the structural integrity of entire systems. In order to appropriately allocate resources and maintain an adequate FAC program, it is necessary to optimize the selection of components for inspection by focusing on those components which provide the best indication of system susceptibility to FAC. A better understanding of system FAC predictability and the types of FAC damage encounteredmore » can provide some of the insight needed to better focus and optimize the inspection plan for an upcoming refueling outage. Laboratory examination of FAC damaged components removed from service at Northeast Utilities` (NU) nuclear power plants provides a better understanding of the damage mechanisms involved and contributing causes. Selected results of this ongoing study are presented with specific conclusions which will help NU to better focus inspections and thus optimize the ongoing FAC inspection program.« less

Influence of the plasma environment on atomic structure using an ion-sphere model

NASA Astrophysics Data System (ADS)

Belkhiri, Madeny; Fontes, Christopher J.; Poirier, Michel

2015-09-01

Plasma environment effects on atomic structure are analyzed using various atomic structure codes. To monitor the effect of high free-electron density or low temperatures, Fermi-Dirac and Maxwell-Boltzmann statistics are compared. After a discussion of the implementation of the Fermi-Dirac approach within the ion-sphere model, several applications are considered. In order to check the consistency of the modifications brought here to extant codes, calculations have been performed using the Los Alamos Cowan Atomic Structure (cats) code in its Hartree-Fock or Hartree-Fock-Slater form and the parametric potential Flexible Atomic Code (fac). The ground-state energy shifts due to the plasma effects for the six most ionized aluminum ions have been calculated using the fac and cats codes and fairly agree. For the intercombination resonance line in Fe22 +, the plasma effect within the uniform electron gas model results in a positive shift that agrees with the multiconfiguration Dirac-Fock value of B. Saha and S. Fritzsche [J. Phys. B 40, 259 (2007), 10.1088/0953-4075/40/2/002]. Last, the present model is compared to experimental data in titanium measured on the terawatt Astra facility and provides values for electron temperature and density in agreement with the maria code.

48 CFR 301.607-76 - FAC-P/PM application process.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false FAC-P/PM application... 301.607-76 FAC-P/PM application process. The P/PM Handbook contains application procedures and forms...; recertification; and certification waiver. Applicants for HHS FAC-P/PM certification actions shall comply with the...

48 CFR 301.607-76 - FAC-P/PM application process.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false FAC-P/PM application... 301.607-76 FAC-P/PM application process. The P/PM Handbook contains application procedures and forms...; recertification; and certification waiver. Applicants for HHS FAC-P/PM certification actions shall comply with the...

48 CFR 301.607-76 - FAC-P/PM application process.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false FAC-P/PM application... 301.607-76 FAC-P/PM application process. The P/PM Handbook contains application procedures and forms...; recertification; and certification waiver. Applicants for HHS FAC-P/PM certification actions shall comply with the...

48 CFR 301.607-76 - FAC-P/PM application process.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false FAC-P/PM application... 301.607-76 FAC-P/PM application process. The P/PM Handbook contains application procedures and forms...; recertification; and certification waiver. Applicants for HHS FAC-P/PM certification actions shall comply with the...

48 CFR 301.607-76 - FAC-P/PM application process.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false FAC-P/PM application... 301.607-76 FAC-P/PM application process. The P/PM Handbook contains application procedures and forms...; recertification; and certification waiver. Applicants for HHS FAC-P/PM certification actions shall comply with the...

Blend Shape Interpolation and FACS for Realistic Avatar

NASA Astrophysics Data System (ADS)

Alkawaz, Mohammed Hazim; Mohamad, Dzulkifli; Basori, Ahmad Hoirul; Saba, Tanzila

2015-03-01

The quest of developing realistic facial animation is ever-growing. The emergence of sophisticated algorithms, new graphical user interfaces, laser scans and advanced 3D tools imparted further impetus towards the rapid advancement of complex virtual human facial model. Face-to-face communication being the most natural way of human interaction, the facial animation systems became more attractive in the information technology era for sundry applications. The production of computer-animated movies using synthetic actors are still challenging issues. Proposed facial expression carries the signature of happiness, sadness, angry or cheerful, etc. The mood of a particular person in the midst of a large group can immediately be identified via very subtle changes in facial expressions. Facial expressions being very complex as well as important nonverbal communication channel are tricky to synthesize realistically using computer graphics. Computer synthesis of practical facial expressions must deal with the geometric representation of the human face and the control of the facial animation. We developed a new approach by integrating blend shape interpolation (BSI) and facial action coding system (FACS) to create a realistic and expressive computer facial animation design. The BSI is used to generate the natural face while the FACS is employed to reflect the exact facial muscle movements for four basic natural emotional expressions such as angry, happy, sad and fear with high fidelity. The results in perceiving the realistic facial expression for virtual human emotions based on facial skin color and texture may contribute towards the development of virtual reality and game environment of computer aided graphics animation systems.

Individualized FAC on bottom tab subassemblies to minimize adhesive gap between emitter and optics

NASA Astrophysics Data System (ADS)

Sauer, Sebastian; Müller, Tobias; Haag, Sebastian; Beleke, Andreas; Zontar, Daniel; Baum, Christoph; Brecher, Christian

2017-02-01

High Power Diode Laser (HPDL) systems with short focal length fast-axis collimators (FAC) require submicron assembly precision. Conventional FAC-Lens assembly processes require adhesive gaps of 50 microns or more in order to compensate for component tolerances (e.g. deviation of back focal length) and previous assembly steps. In order to control volumetric shrinkage of fast-curing UV-adhesives shrinkage compensation is mandatory. The novel approach described in this paper aims to minimize the impact of volumetric shrinkage due to the adhesive gap between HPDL edge emitters and FAC-Lens. Firstly, the FAC is actively aligned to the edge emitter without adhesives or bottom tab. The relative position and orientation of FAC to emitter are measured and stored. Consecutively, an individual subassembly of FAC and bottom tab is assembled on Fraunhofer IPT's mounting station with a precision of +/-1 micron. Translational and lateral offsets can be compensated, so that a narrow and uniform glue gap for the consecutive bonding process of bottom tab to heatsink applies (Figure 4). Accordingly, FAC and bottom tab are mounted to the heatsink without major shrinkage compensation. Fraunhofer IPT's department assembly of optical systems and automation has made several publications regarding active alignment of FAC lenses [SPIE LASE 8241-12], volumetric shrinkage compensation [SPIE LASE 9730-28] and FAC on bottom tab assembly [SPIE LASE 9727-31] in automated production environments. The approach described in this paper combines these and is the logical continuation of that work towards higher quality of HPDLs.

VizieR Online Data Catalog: Energy levels & transition rates for F-like ions (Si+, 2016)

NASA Astrophysics Data System (ADS)

Si, R.; Li, S.; Guo, X. L.; Chen, Z. B.; Brage, T.; Jonsson, P.; Wang, K.; Yan, J.; Chen, C. Y.; Zou, Y. M.

2017-01-01

For the multiconfiguration Dirac-Hartree-Fock (MCDHF) calculation we use the latest version of the GRASP2K code (Jonsson+ 2013CoPhC.184.2197J), while the many-body perturbation theory (MBPT) calculation is performed using the Flexible Atomic Code (FAC; Gu 2008CaJPh..86..675G). (2 data files).

Possibility of Ionospheric Cause of FACs and Convection Field in the Magnetosphere-Ionosphere System: The Harang Reversal, Premidnight Upward-FAC, and the Ionospheric Hall Polarization Field

NASA Astrophysics Data System (ADS)

Nakamizo, A.; Yoshikawa, A.

2016-12-01

Whereas it is generally thought that Birkeland Currents (FACs) are generated in the magnetosphere and that the ionospheric convection reflects the magnetospheric convection, we present a possibility that the ionosphere drives FACs and the convection field in the M-I system. We apply this idea to the Harang Reversal (HR) for demonstration. By using an ionospheric potential solver we calculate the electrostatic field for given distributions of FACs and conductance. The result shows that a conspicuous structure resembling HR is generated even for a symmetric distribution of the R1-type FACs and that the Hall polarization field is produced at the equatorward boundary of the auroral region as the primary currents diverge/converge at the conductance gradient there, which causes the potential deformation (HR). Conventionally HR has been considered to be of the magnetospheric origin, and a ring current model actually produces the corresponding structure in the magnetosphere [e.g., Erickson et al., 1991]. Observationally the divE equivalent to HR is consistent with the premidnight upward-FAC seen in Iijima and Potemra's diagram. A recent theoretical study [Ohtani et al., 2016] proposes that HR is a required structure for the interchange stability of the magnetotail in the presence of the R1 and R2-FAC systems including a premidnight upward-FAC. Returning to our result, the important point is that HR is reproduced at the conductance edge by the ionospheric polarization field, for which the primary field originates from the R1-FACs distributed far from that region. We also suggest: (i) In a more realistic finite ΣA, the total ionospheric polarization is partly released by a FAC, which may be a part of the premidnight upward-FAC. (ii) However, existing simulation models do not allow this type of current closure, and accordingly they may enhance the HR structure in the magnetosphere. This discussion should hold generally and would promote the global M-I coupling studies to the next step.

78 FR 58449 - Generator Requirements at the Transmission Interface

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-24

... Connection Requirements), FAC-003-3 (Transmission Vegetation Management), PRC-004- 2.1a (Analysis and... and Generation Protection System Maintenance and Testing). The modifications improve reliability... Standards FAC-001-1 (Facility Connection Requirements), FAC-003-3 (Transmission Vegetation Management), PRC...

Predicting, examining, and evaluating FAC in US power plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cohn, M.J.; Garud, Y.S.; Raad, J. de

1999-11-01

There have been many pipe failures in fossil and nuclear power plant piping systems caused by flow-accelerated corrosion (FAC). In some piping systems, this failure mechanism maybe the most important type of damage to mitigate because FAC damage has led to catastrophic failures and fatalities. Detecting the damage and mitigating the problem can significantly reduce future forced outages and increase personnel safety. This article discusses the implementation of recent developments to select FAC inspection locations, perform cost-effective examinations, evaluate results, and mitigate FAC failures. These advances include implementing the combination of software to assist in selecting examination locations and anmore » improved pulsed eddy current technique to scan for wall thinning without removing insulation. The use of statistical evaluation methodology and possible mitigation strategies also are discussed.« less

Thermally induced defluorination during a mer to fac transformation of a blue-green phosphorescent cyclometalated iridium(III) complex.

PubMed

Zheng, Yonghao; Batsanov, Andrei S; Edkins, Robert M; Beeby, Andrew; Bryce, Martin R

2012-01-02

The new homoleptic tris-cyclometalated [Ir(C^N)(3)] complexes mer-8, fac-8, and fac-9 incorporating γ-carboline ligands are reported. Reaction of 3-(2,4-difluorophenyl)-5-(2-ethylhexyl)-pyrido[4,3-b]indole 6 with iridium(III) chloride under standard cyclometalating conditions gave the homoleptic complex mer-8 in 63% yield. The X-ray crystal structure of mer-8 is described. The Ir-C and Ir-N bonds show the expected bond length alternations for the differing trans influence of phenyl and pyridyl ligands. mer-8 quantitatively isomerized to fac-8 upon irradiation with UV light. However, heating mer-8 at 290 °C in glycerol led to an unusual regioselective loss of one fluorine atom from each of the ligands, yielding fac-9 in 58% yield. fac-8 is thermally very stable: no decomposition was observed when fac-8 was heated in glycerol at 290 °C for 48 h. The γ-carboline system of fac-8 enhances thermal stability compared to the pyridyl analogue fac-Ir(46dfppy)(3)10, which decomposes extensively upon being heated in glycerol at 290 °C for 2 h. Complexes mer-8, fac-8, and fac-9 are emitters of blue-green light (λ(max)(em) = 477, 476, and 494 nm, respectively). The triplet lifetimes for fac-8 and fac-9 are ~4.5 μs at room temperature; solution Φ(PL) values are 0.31 and 0.22, respectively.

48 CFR 301.603-72 - FAC-C and HHS SAC certification requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... HUMAN SERVICES GENERAL HHS ACQUISITION REGULATION SYSTEM Career Development, Contracting Authority, and... retention of certification, including the requirement to earn continuous learning points (CLPs). FAC-C... to employees for the first time at a department or agency.) (c) The FAC-C certification is based on...

The application of silicalite-1/fly ash cenosphere (S/FAC) zeolite composite for the adsorption of methyl tert-butyl ether (MTBE).

PubMed

Lu, Jia; Xu, Fang; Wang, Deju; Huang, Jue; Cai, Weimin

2009-06-15

Silicalite-1/fly ash cenosphere (S/FAC) zeolite composite has been applied for batch adsorption of methyl tert-butyl ether (MTBE) from water systems. Here the key experimental conditions, including the ratio of initial MTBE concentration to the amount weight of S/FAC, adsorption time and temperature, have been discussed in detail. The results show that approximately 93-95% MTBE could be adsorbed with initial concentration of MTBE solution 1000 microg l(-1). The column flow-through experiments also prove the high capacity of S/FAC composite for MTBE removal. The distinct advantages of S/FAC zeolite composite as adsorbent lie in (1) enhanced adsorption rate and capacity based on hierarchical micro and meso/macroporosity of S/FAC; (2) more easily operation and recycling process by assembly of nano-sized silicalite-1 zeolite on FAC support.

Flow Accelerated Erosion-Corrosion (FAC) considerations for secondary side piping in the AP1000{sup R} nuclear power plant design

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vanderhoff, J. F.; Rao, G. V.; Stein, A.

2012-07-01

The issue of Flow Accelerated Erosion-Corrosion (FAC) in power plant piping is a known phenomenon that has resulted in material replacements and plant accidents in operating power plants. Therefore, it is important for FAC resistance to be considered in the design of new nuclear power plants. This paper describes the design considerations related to FAC that were used to develop a safe and robust AP1000{sup R} plant secondary side piping design. The primary FAC influencing factors include: - Fluid Temperature - Pipe Geometry/layout - Fluid Chemistry - Fluid Velocity - Pipe Material Composition - Moisture Content (in steam lines) Duemore » to the unknowns related to the relative impact of the influencing factors and the complexities of the interactions between these factors, it is difficult to accurately predict the expected wear rate in a given piping segment in a new plant. This paper provides: - a description of FAC and the factors that influence the FAC degradation rate, - an assessment of the level of FAC resistance of AP1000{sup R} secondary side system piping, - an explanation of options to increase FAC resistance and associated benefits/cost, - discussion of development of a tool for predicting FAC degradation rate in new nuclear power plants. (authors)« less

An Overview of the Formation and Attitude Control System for the Terrestrial Planet Finder Formation Flying Interferometer

NASA Technical Reports Server (NTRS)

Scharf, Daniel P.; Hadaegh, Fred Y.; Rahman, Zahidul H.; Shields, Joel F.; Singh, Gurkipal

2004-01-01

The Terrestrial Planet Finder formation flying Interferometer (TPF-I) will be a five-spacecraft, precision formation operating near a Sun-Earth Lagrange point. As part of technology development for TPF-I, a formation and attitude control system (FACS) is being developed that achieves the precision and functionality associated with the TPF-I formation. This FACS will be demonstrated in a distributed, real-time simulation environment. In this paper we present an overview of the FACS and discuss in detail its constituent formation estimation, guidance and control architectures and algorithms. Since the FACS is currently being integrated into a high-fidelity simulation environment, component simulations demonstrating algorithm performance are presented.

Automated and objective action coding of facial expressions in patients with acute facial palsy.

PubMed

Haase, Daniel; Minnigerode, Laura; Volk, Gerd Fabian; Denzler, Joachim; Guntinas-Lichius, Orlando

2015-05-01

Aim of the present observational single center study was to objectively assess facial function in patients with idiopathic facial palsy with a new computer-based system that automatically recognizes action units (AUs) defined by the Facial Action Coding System (FACS). Still photographs using posed facial expressions of 28 healthy subjects and of 299 patients with acute facial palsy were automatically analyzed for bilateral AU expression profiles. All palsies were graded with the House-Brackmann (HB) grading system and with the Stennert Index (SI). Changes of the AU profiles during follow-up were analyzed for 77 patients. The initial HB grading of all patients was 3.3 ± 1.2. SI at rest was 1.86 ± 1.3 and during motion 3.79 ± 4.3. Healthy subjects showed a significant AU asymmetry score of 21 ± 11 % and there was no significant difference to patients (p = 0.128). At initial examination of patients, the number of activated AUs was significantly lower on the paralyzed side than on the healthy side (p < 0.0001). The final examination for patients took place 4 ± 6 months post baseline. The number of activated AUs and the ratio between affected and healthy side increased significantly between baseline and final examination (both p < 0.0001). The asymmetry score decreased between baseline and final examination (p < 0.0001). The number of activated AUs on the healthy side did not change significantly (p = 0.779). Radical rethinking in facial grading is worthwhile: automated FACS delivers fast and objective global and regional data on facial motor function for use in clinical routine and clinical trials.

Universals and cultural variations in 22 emotional expressions across five cultures.

PubMed

Cordaro, Daniel T; Sun, Rui; Keltner, Dacher; Kamble, Shanmukh; Huddar, Niranjan; McNeil, Galen

2018-02-01

We collected and Facial Action Coding System (FACS) coded over 2,600 free-response facial and body displays of 22 emotions in China, India, Japan, Korea, and the United States to test 5 hypotheses concerning universals and cultural variants in emotional expression. New techniques enabled us to identify cross-cultural core patterns of expressive behaviors for each of the 22 emotions. We also documented systematic cultural variations of expressive behaviors within each culture that were shaped by the cultural resemblance in values, and identified a gradient of universality for the 22 emotions. Our discussion focused on the science of new expressions and how the evidence from this investigation identifies the extent to which emotional displays vary across cultures. (PsycINFO Database Record (c) 2018 APA, all rights reserved).

Comparison of field-aligned currents at ionospheric and magnetospheric altitudes

NASA Technical Reports Server (NTRS)

Spence, H. E.; Kivelson, M. G.; Walker, R. J.

1988-01-01

Using the empirical terrestrial magnetospheric magnetic field models of Tsyganenko and Usmanov (1982) and Tsyganenko (1987) the average field-aligned currents (FACs) in the magnetosphere were determined as a function of the Kp index. Three major model FAC systems were identified, namely, the dayside region 1, the nightside region 1, and the nightside polar cap. The models provide information about the sources of the current systems. Mapped ionospheric model FACs are compared with low-altitude measurements obtained by the spacecraft. It is found that low-altitude data can reveal either classic region 1/2 or more highly structured FAC patterns. Therefore, statistical results either obtained from observations or inferred from models are expected to be averages over temporally and spatially shifting patterns.

Measuring Facial Movement

ERIC Educational Resources Information Center

Ekman, Paul; Friesen, Wallace V.

1976-01-01

The Facial Action Code (FAC) was derived from an analysis of the anatomical basis of facial movement. The development of the method is explained, contrasting it to other methods of measuring facial behavior. An example of how facial behavior is measured is provided, and ideas about research applications are discussed. (Author)

Classifying Facial Actions

PubMed Central

Donato, Gianluca; Bartlett, Marian Stewart; Hager, Joseph C.; Ekman, Paul; Sejnowski, Terrence J.

2010-01-01

The Facial Action Coding System (FACS) [23] is an objective method for quantifying facial movement in terms of component actions. This system is widely used in behavioral investigations of emotion, cognitive processes, and social interaction. The coding is presently performed by highly trained human experts. This paper explores and compares techniques for automatically recognizing facial actions in sequences of images. These techniques include analysis of facial motion through estimation of optical flow; holistic spatial analysis, such as principal component analysis, independent component analysis, local feature analysis, and linear discriminant analysis; and methods based on the outputs of local filters, such as Gabor wavelet representations and local principal components. Performance of these systems is compared to naive and expert human subjects. Best performances were obtained using the Gabor wavelet representation and the independent component representation, both of which achieved 96 percent accuracy for classifying 12 facial actions of the upper and lower face. The results provide converging evidence for the importance of using local filters, high spatial frequencies, and statistical independence for classifying facial actions. PMID:21188284

Extreme ultraviolet emission spectra of Gd and Tb ions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kilbane, D.; O'Sullivan, G.

2010-11-15

Theoretical extreme ultraviolet emission spectra of gadolinium and terbium ions calculated with the Cowan suite of codes and the flexible atomic code (FAC) relativistic code are presented. 4d-4f and 4p-4d transitions give rise to unresolved transition arrays in a range of ions. The effects of configuration interaction are investigated for transitions between singly excited configurations. Optimization of emission at 6.775 nm and 6.515 nm is achieved for Gd and Tb ions, respectively, by consideration of plasma effects. The resulting synthetic spectra are compared with experimental spectra recorded using the laser produced plasma technique.

Chimpanzees (Pan troglodytes) Produce the Same Types of ‘Laugh Faces’ when They Emit Laughter and when They Are Silent

PubMed Central

Davila-Ross, Marina; Jesus, Goncalo; Osborne, Jade; Bard, Kim A.

2015-01-01

The ability to flexibly produce facial expressions and vocalizations has a strong impact on the way humans communicate, as it promotes more explicit and versatile forms of communication. Whereas facial expressions and vocalizations are unarguably closely linked in primates, the extent to which these expressions can be produced independently in nonhuman primates is unknown. The present work, thus, examined if chimpanzees produce the same types of facial expressions with and without accompanying vocalizations, as do humans. Forty-six chimpanzees (Pan troglodytes) were video-recorded during spontaneous play with conspecifics at the Chimfunshi Wildlife Orphanage. ChimpFACS was applied, a standardized coding system to measure chimpanzee facial movements, based on FACS developed for humans. Data showed that the chimpanzees produced the same 14 configurations of open-mouth faces when laugh sounds were present and when they were absent. Chimpanzees, thus, produce these facial expressions flexibly without being morphologically constrained by the accompanying vocalizations. Furthermore, the data indicated that the facial expression plus vocalization and the facial expression alone were used differently in social play, i.e., when in physical contact with the playmates and when matching the playmates’ open-mouth faces. These findings provide empirical evidence that chimpanzees produce distinctive facial expressions independently from a vocalization, and that their multimodal use affects communicative meaning, important traits for a more explicit and versatile way of communication. As it is still uncertain how human laugh faces evolved, the ChimpFACS data were also used to empirically examine the evolutionary relation between open-mouth faces with laugh sounds of chimpanzees and laugh faces of humans. The ChimpFACS results revealed that laugh faces of humans must have gradually emerged from laughing open-mouth faces of ancestral apes. This work examines the main evolutionary changes of laugh faces since the last common ancestor of chimpanzees and humans. PMID:26061420

Chimpanzees (Pan troglodytes) Produce the Same Types of 'Laugh Faces' when They Emit Laughter and when They Are Silent.

PubMed

Davila-Ross, Marina; Jesus, Goncalo; Osborne, Jade; Bard, Kim A

2015-01-01

The ability to flexibly produce facial expressions and vocalizations has a strong impact on the way humans communicate, as it promotes more explicit and versatile forms of communication. Whereas facial expressions and vocalizations are unarguably closely linked in primates, the extent to which these expressions can be produced independently in nonhuman primates is unknown. The present work, thus, examined if chimpanzees produce the same types of facial expressions with and without accompanying vocalizations, as do humans. Forty-six chimpanzees (Pan troglodytes) were video-recorded during spontaneous play with conspecifics at the Chimfunshi Wildlife Orphanage. ChimpFACS was applied, a standardized coding system to measure chimpanzee facial movements, based on FACS developed for humans. Data showed that the chimpanzees produced the same 14 configurations of open-mouth faces when laugh sounds were present and when they were absent. Chimpanzees, thus, produce these facial expressions flexibly without being morphologically constrained by the accompanying vocalizations. Furthermore, the data indicated that the facial expression plus vocalization and the facial expression alone were used differently in social play, i.e., when in physical contact with the playmates and when matching the playmates' open-mouth faces. These findings provide empirical evidence that chimpanzees produce distinctive facial expressions independently from a vocalization, and that their multimodal use affects communicative meaning, important traits for a more explicit and versatile way of communication. As it is still uncertain how human laugh faces evolved, the ChimpFACS data were also used to empirically examine the evolutionary relation between open-mouth faces with laugh sounds of chimpanzees and laugh faces of humans. The ChimpFACS results revealed that laugh faces of humans must have gradually emerged from laughing open-mouth faces of ancestral apes. This work examines the main evolutionary changes of laugh faces since the last common ancestor of chimpanzees and humans.

Influence of the IMF Cone Angle on Invariant Latitudes of Polar Region Footprints of FACs in the Magnetotail: Cluster Observation

NASA Astrophysics Data System (ADS)

Cheng, Z. W.; Shi, J. K.; Zhang, J. C.; Torkar, K.; Kistler, L. M.; Dunlop, M.; Carr, C.; Rème, H.; Dandouras, I.; Fazakerley, A.

2018-04-01

The influence of the interplanetary magnetic field (IMF) cone angle θ (the angle between the IMF direction and the Sun-Earth line) on the invariant latitudes of the footprints of the field-aligned currents (FACs) in the magnetotail has been investigated. We performed a statistical study of 542 FAC cases observed by the four Cluster spacecraft in the Northern Hemisphere. The results show that there are almost no FACs when the IMF cone angle is less than 10°, and there are indications of the FACs in the plasma sheet boundary layers being weak under the radial IMF conditions. The footprints of the large FAC (>10 nA/m2) cases are within invariant latitudes <71° and mainly within IMF cone angles θ > 60°, which implies that the footprints of the large FACs mainly expand equatorward with large IMF cone angle. The equatorward boundary of the FAC footprints in the polar region decreases with increasing IMF cone angle (and has a better correlation for northward IMF), which shows that the IMF cone angle plays an important controlling role in FAC distributions in the magnetosphere-ionosphere coupling system. There is almost no correlation between the poleward boundary and the IMF cone angle for both northward and southward IMF. This is because the poleward boundary movement is limited by an enhanced lobe magnetic flux. This is the first time a correlation between FAC footprints in the polar region and IMF cone angles has been determined.

Field-Aligned Current Systems at Mercury

NASA Astrophysics Data System (ADS)

Heyner, Daniel; Exner, Willi

2017-04-01

Mercury exhibits a very dynamic magnetosphere, which is partially due to strong dayside reconnection and fast magnetospheric convection. It has been shown that dayside reconnection occurs even on low magnetic shear angles across the magnetopause. This drives quasi-steady region 1 field-aligned currents (FAC) that are observable in in-situ MESSENGER data. Here, the structure of the Hermean FAC-system is discussed and compared to the terrestrial counterpart. Due to the lack of a significant ionosphere at Mercury, it has to be examined how much of the poloidal FAC is reflected back to the magnetosphere, closed via toroidal currents in the planetary interior or via Pedersen currents in the tenuous exosphere. This investigation gives insights into the planetary conductivity structure as well as the exospheric plasma densities. Furthermore, it will be examined how much the only partially developed ring current at Mercury produces possible region 2 FAC signatures. We conclude with requirements to simulations that are needed to forecast the FAC structure on the southern hemisphere that will be closely studied with the upcoming BepiColombo mission.

Ocular Pharmacokinetics of Fluocinolone Acetonide Following Iluvien Implantation in the Vitreous Humor of Rabbits

PubMed Central

Kane, Frances E.

2015-01-01

Abstract Purpose: The purpose of this study was to evaluate the systemic and ocular pharmacokinetics (PK) of fluocinolone acetonide (FAc) following administration of Iluvien® intravitreal implants. Methods: The FAc intravitreal implant was administered to rabbits in 3 doses (0.2, 0.5, and 1.0 μg/day). The concentration of FAc was measured by a validated liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method in plasma and ocular tissues at various time points through month 24. Results: Following administration of the 0.2 μg/day implant, FAc levels peaked in most tissues at day 2 or 8, reached approximate steady state levels by month 3 and very gradually decreased over the duration of the study. The FAc level in the aqueous humor was not measurable at most time points in the rabbit. FAc was still present in most ocular tissues at 2 years. The 0.5 and 1.0 μg/day dose groups followed the same pattern through month 9. The elimination half lives in the tissues for which it was measurable were greater than 83 days. Exposure to FAc was highest in the choroid/retinal pigment epithelium for all doses, followed by lens and retina. Conclusions: The results of this study demonstrate sustained delivery of FAc from the Iluvien intravitreal implant in the ocular tissue of rabbits. Retina and lens FAc levels with the Iluvien implant were approximately 1/10 those reported with the Retisert® implant. FAc levels in the aqueous were not measureable with Iluvien where they were measured for 12 months with Retisert. PMID:25562126

A study of patient facial expressivity in relation to orthodontic/surgical treatment.

PubMed

Nafziger, Y J

1994-09-01

A dynamic analysis of the faces of patients seeking an aesthetic restoration of facial aberrations with orthognathic treatment requires (besides the routine static study, such as records, study models, photographs, and cephalometric tracings) the study of their facial expressions. To determine a classification method for the units of expressive facial behavior, the mobility of the face is studied with the aid of the facial action coding system (FACS) created by Ekman and Friesen. With video recordings of faces and photographic images taken from the video recordings, the authors have modified a technique of facial analysis structured on the visual observation of the anatomic basis of movement. The technique, itself, is based on the defining of individual facial expressions and then codifying such expressions through the use of minimal, anatomic action units. These action units actually combine to form facial expressions. With the help of FACS, the facial expressions of 18 patients before and after orthognathic surgery, and six control subjects without dentofacial deformation have been studied. I was able to register 6278 facial expressions and then further define 18,844 action units, from the 6278 facial expressions. A classification of the facial expressions made by subject groups and repeated in quantified time frames has allowed establishment of "rules" or "norms" relating to expression, thus further enabling the making of comparisons of facial expressiveness between patients and control subjects. This study indicates that the facial expressions of the patients were more similar to the facial expressions of the controls after orthognathic surgery. It was possible to distinguish changes in facial expressivity in patients after dentofacial surgery, the type and degree of change depended on the facial structure before surgery. Changes noted tended toward a functioning that is identical to that of subjects who do not suffer from dysmorphosis and toward greater lip competence, particularly the function of the orbicular muscle of the lips, with reduced compensatory activity of the lower lip and the chin. The results of our study are supported by the clinical observations and suggest that the FACS technique should be able to provide a coding for the study of facial expression.

Four large-scale field-aligned current systmes in the dayside high-latitude region

NASA Technical Reports Server (NTRS)

Ohtani, S.; Potemra, T. A.; Newell, P.T.; Zanetti, L. J.; Iijima, T.; Watanabe, M.; Blomberg, L. G.; Elphinstone, R. D.; Murphree, J. S.; Yamauchi, M.

1995-01-01

A system of four current sheets of large-scale field-aligned currents (FACs) was discovered in the data set of simultaneous Viking and Defense Meteorological Satellire Program-F7 (DMSP-F7) crossing of the dayside high-latitude region. This paper reports four examples of this system that were observed in the prenoon sector. The flow polarities of FACs are upward, downward, upward, and downward, from equatorward to poleward. The lowest-latitude upward current is flowing mostly in the central plasma sheet (CPS) precipitation region, often overlapping with the boundary plasma sheet (BPS) at its poleward edge, andis interpreted as a region 2 current. The pair of downward and upward FACs in the middle of te structure are collocated with structured electron precipitation. The precipitation of high-energy (greater than 1 keV) electrons is more intense in the lower-latitude downward current sheet. The highest-latitude downward flowing current sheet is located in a weak, low-energy particle precipitation region, suggesting that this current is flowing on open field lines. Simulaneous observations in the postnoon local time sector reveal the standard three-sheet structure of FACs, sometimes described as region 2, region 1, and mantle (referred to the midday region O) currents. A high correlation was found between the occurrence of the four FAC sheet structure and negative interplanetary magnetic field (IMF) B(sub Y). We discuss the FAC structurein terms of three types of convection cells: the merging, viscous, andlobe cells. During strongly negative IMF B(sub Y), two convection reversals exist in the prenoon sector; one is inside the viscous cell, and the other is between the viscous cell and the lobe cell. This structure of convection flow is supported by the Viking electric field and auroral UV image data. Based on the convection pattern, the four FAC sheet structure is interpreted as the latitude overlap of midday and morning FAC systems. We suggest that the for-current sheet structure is common in a certain prenoon localtime sector during strongly negative IMF B(sub Y).

Wetland Plants of Specialized Habitats in the Arid West

DTIC Science & Technology

2007-06-01

NO FACW NO NO FACW Xanthium strumarium L. FAC- NI FAC FAC FAC+ Yucca brevifolia Engelm. UPL UPL UPL UPL UPL 1 Barbour and Billings (1988) 7...FACW FAC Washingtonia filifera (L. Linden) H. Wendl. 2,7 NO FACW NO NO FACW Xanthium strumarium L. FAC- NI FAC FAC FAC+ Yucca brevifolia

High-latitude Pi2 pulsations associated with kink-like neutral sheet oscillations

NASA Astrophysics Data System (ADS)

Wang, G. Q.; Volwerk, M.; Zhang, T. L.; Schmid, D.; Yoshikawa, A.

2017-03-01

A kink-like neutral sheet oscillation event observed by Cluster between 1436 and 1445 UT on 15 October 2004 has been investigated. The oscillations with periods between 40 and 60 s, observed at (-13.1, 8.7, -0.5) RE, are dominant in BX and BY. And they propagate mainly duskward with a velocity of (86, 147, 46) km/s. Their periods and velocity can be explained by the magnetic double-gradient instability. These oscillations are accompanied by strong field-aligned currents (FACs), which prefer to occur near the strongly tilted current sheet, and local maximum FAC tends to occur near the neutral sheet. The FACs show one-to-one correlated with a high-latitude Pi2 pulsation event recorded by KTN and TIK stations with a delay time of 60 and 90 s, respectively. Both the Pi2 and oscillations propagate westward with a comparative conjunctive speed. These findings suggest a strong relation between the FACs and Pi2, and we infer that the Pi2 is caused by the FACs. The periods of the FACs are modulated by the oscillations but not exactly equal, which is one possible reason that the period of the Pi2 caused by the FACs could be different from the oscillations. We speculate that a current circuit between the plasma sheet and ionosphere can be formed during strongly tilted current sheet, and successive tilted current sheet could generate quasiperiodic multiple FAC systems, which can generate high-latitude Pi2 pulsations and control their periods.

Energy levels, radiative rates and electron impact excitation rates for transitions in He-like Ga XXX, Ge XXXI, As XXXII, Se XXXIII and Br XXXIV

NASA Astrophysics Data System (ADS)

Aggarwal, Kanti M.; Keenan, Francis P.

2013-04-01

We report calculations of energy levels, radiative rates and electron impact excitation cross sections and rates for transitions in He-like Ga XXX, Ge XXXI, As XXXII, Se XXXIII and Br XXXIV. The grasp (general-purpose relativistic atomic structure package) is adopted for calculating energy levels and radiative rates. For determining the collision strengths, and subsequently the excitation rates, the Dirac atomic R-matrix code (darc) is used. Oscillator strengths, radiative rates and line strengths are reported for all E1, E2, M1 and M2 transitions among the lowest 49 levels of each ion. Additionally, theoretical lifetimes are provided for all 49 levels of the above five ions. Collision strengths are averaged over a Maxwellian velocity distribution and the effective collision strengths obtained listed over a wide temperature range up to 108 K. Comparisons are made with similar data obtained using the flexible atomic code (fac) to highlight the importance of resonances, included in calculations with darc, in the determination of effective collision strengths. Discrepancies between the collision strengths from darc and fac, particularly for some forbidden transitions, are also discussed. Finally, discrepancies between the present results for effective collision strengths with the darc code and earlier semi-relativistic R-matrix data are noted over a wide range of electron temperatures for many transitions in all ions.

Muecas: A Multi-Sensor Robotic Head for Affective Human Robot Interaction and Imitation

PubMed Central

Cid, Felipe; Moreno, Jose; Bustos, Pablo; Núñez, Pedro

2014-01-01

This paper presents a multi-sensor humanoid robotic head for human robot interaction. The design of the robotic head, Muecas, is based on ongoing research on the mechanisms of perception and imitation of human expressions and emotions. These mechanisms allow direct interaction between the robot and its human companion through the different natural language modalities: speech, body language and facial expressions. The robotic head has 12 degrees of freedom, in a human-like configuration, including eyes, eyebrows, mouth and neck, and has been designed and built entirely by IADeX (Engineering, Automation and Design of Extremadura) and RoboLab. A detailed description of its kinematics is provided along with the design of the most complex controllers. Muecas can be directly controlled by FACS (Facial Action Coding System), the de facto standard for facial expression recognition and synthesis. This feature facilitates its use by third party platforms and encourages the development of imitation and of goal-based systems. Imitation systems learn from the user, while goal-based ones use planning techniques to drive the user towards a final desired state. To show the flexibility and reliability of the robotic head, the paper presents a software architecture that is able to detect, recognize, classify and generate facial expressions in real time using FACS. This system has been implemented using the robotics framework, RoboComp, which provides hardware-independent access to the sensors in the head. Finally, the paper presents experimental results showing the real-time functioning of the whole system, including recognition and imitation of human facial expressions. PMID:24787636

An Overview of the Formation and Attitude Control System for the Terrestrial Planet Finder Formation Flying Interferometer

NASA Technical Reports Server (NTRS)

Scharf, Daniel P.; Hadaegh, Fred Y.; Rahman, Zahidul H.; Shields, Joel F.; Singh, Gurkipal; Wette, Matthew R.

2004-01-01

The Terrestrial Planet Finder formation flying Interferometer (TPF-I) will be a five-spacecraft, precision formation operating near the second Sun-Earth Lagrange point. As part of technology development for TPF-I, a formation and attitude control system (FACS) is being developed that achieves the precision and functionality needed for the TPF-I formation and that will be demonstrated in a distributed, real-time simulation environment. In this paper we present an overview of FACS and discuss in detail its formation estimation, guidance and control architectures and algorithms. Since FACS is currently being integrated into a high-fidelity simulation environment, component simulations demonstrating algorithm performance are presented.

Automated assembly of fast-axis collimation (FAC) lenses for diode laser bar modules

NASA Astrophysics Data System (ADS)

Miesner, Jörn; Timmermann, Andre; Meinschien, Jens; Neumann, Bernhard; Wright, Steve; Tekin, Tolga; Schröder, Henning; Westphalen, Thomas; Frischkorn, Felix

2009-02-01

Laser diodes and diode laser bars are key components in high power semiconductor lasers and solid state laser systems. During manufacture, the assembly of the fast axis collimation (FAC) lens is a crucial step. The goal of our activities is to design an automated assembly system for high volume production. In this paper the results of an intermediate milestone will be reported: a demonstration system was designed, realized and tested to prove the feasibility of all of the system components and process features. The demonstration system consists of a high precision handling system, metrology for process feedback, a powerful digital image processing system and tooling for glue dispensing, UV curing and laser operation. The system components as well as their interaction with each other were tested in an experimental system in order to glean design knowledge for the fully automated assembly system. The adjustment of the FAC lens is performed by a series of predefined steps monitored by two cameras concurrently imaging the far field and the near field intensity distributions. Feedback from these cameras processed by a powerful and efficient image processing algorithm control a five axis precision motion system to optimize the fast axis collimation of the laser beam. Automated cementing of the FAC to the diode bar completes the process. The presentation will show the system concept, the algorithm of the adjustment as well as experimental results. A critical discussion of the results will close the talk.

Statistical Comparisons of Meso- and Small-Scale Field-Aligned Currents with Auroral Electron Acceleration Mechanisms from FAST Observations

NASA Astrophysics Data System (ADS)

Dombeck, J. P.; Cattell, C. A.; Prasad, N.; Sakher, A.; Hanson, E.; McFadden, J. P.; Strangeway, R. J.

2016-12-01

Field-aligned currents (FACs) provide a fundamental driver and means of Magnetosphere-Ionosphere (M-I) coupling. These currents need to be supported by local physics along the entire field line generally with quasi-static potential structures, but also supporting the time-evolution of the structures and currents, producing Alfvén waves and Alfvénic electron acceleration. In regions of upward current, precipitating auroral electrons are accelerated earthward. These processes can result in ion outflow, changes in ionospheric conductivity, and affect the particle distributions on the field line, affecting the M-I coupling processes supporting the individual FACs and potentially the entire FAC system. The FAST mission was well suited to study both the FACs and the electron auroral acceleration processes. We present the results of the comparisons between meso- and small-scale FACs determined from FAST using the method of Peria, et al., 2000, and our FAST auroral acceleration mechanism study when such identification is possible for the entire ˜13 year FAST mission. We also present the latest results of the electron energy (and number) flux ionospheric input based on acceleration mechanism (and FAC characteristics) from our FAST auroral acceleration mechanism study.

Two Way Coupling RAM-SCB to the Space Weather Modeling Framework

NASA Astrophysics Data System (ADS)

Welling, D. T.; Jordanova, V. K.; Zaharia, S. G.; Toth, G.

2010-12-01

The Ring current Atmosphere interaction Model with Self-Consistently calculated 3D Magnetic field (RAM-SCB) has been used to successfully study inner magnetosphere dynamics during different solar wind and magnetosphere conditions. Recently, one way coupling of RAM-SCB with the Space Weather Modeling Framework (SWMF) has been achieved to replace all data or empirical inputs with those obtained through first-principles-based codes: magnetic field and plasma flux outer boundary conditions are provided by the Block Adaptive Tree Solar wind Roe-type Upwind Scheme (BATS-R-US) MHD code, convection electric field is provided by the Ridley Ionosphere Model (RIM), and ion composition is provided by the Polar Wind Outflow Model (PWOM) combined with a multi-species MHD approach. These advances, though creating a powerful inner magnetosphere virtual laboratory, neglect the important mechanisms through which the ring current feeds back into the whole system, primarily the stretching of the magnetic field lines and shielding of the convection electric field through strong region two Field Aligned Currents (FACs). In turn, changing the magnetosphere in this way changes the evolution of the ring current. To address this shortcoming, the coupling has been expanded to include feedback from RAM-SCB to the other coupled codes: region two FACs are returned to the RIM while total plasma pressure is used to nudge the MHD solution towards the RAM-SCB values. The impacts of the two way coupling are evaluated on three levels: the global magnetospheric level, focusing on the impact on the ionosphere and the shape of the magnetosphere, the regional level, examining the impact on the development of the ring current in terms of energy density, anisotropy, and plasma distribution, and the local level to compare the new results to in-situ measurements of magnetic and electric field and plasma. The results will also be compared to past simulations using the one way coupling and no coupling whatsoever. This work is the first to fully couple an anisotropic kinetic ring current code with a self-consistently calculated magnetic field to a set of global models.

Two way coupling RAM-SCB to the space weather modeling framework

DOE Office of Scientific and Technical Information (OSTI.GOV)

Welling, Daniel T; Jordanova, Vania K; Zaharia, Sorin G

The Ring current Atmosphere interaction Model with Self-Consistently calculated 3D Magnetic field (RAM-SCB) has been used to successfully study inner magnetosphere dynamics during different solar wind and magnetosphere conditions. Recently, one way coupling of RAM-SCB with the Space Weather Modeling Framework (SWMF) has been achieved to replace all data or empirical inputs with those obtained through first-principles-based codes: magnetic field and plasma flux outer boundary conditions are provided by the Block Adaptive Tree Solar wind Roe-type Upwind Scheme (BATS-R-US) MHO code, convection electric field is provided by the Ridley Ionosphere Model (RIM), and ion composition is provided by the Polarmore » Wind Outflow Model (PWOM) combined with a multi-species MHO approach. These advances, though creating a powerful inner magnetosphere virtual laboratory, neglect the important mechanisms through which the ring current feeds back into the whole system, primarily the stretching of the magnetic field lines and shielding of the convection electric field through strong region two Field Aligned Currents (FACs). In turn, changing the magnetosphere in this way changes the evolution of the ring current. To address this shortcoming, the coupling has been expanded to include feedback from RAM-SCB to the other coupled codes: region two FACs are returned to the RIM while total plasma pressure is used to nudge the MHO solution towards the RAMSCB values. The impacts of the two way coupling are evaluated on three levels: the global magnetospheric level, focusing on the impact on the ionosphere and the shape of the magnetosphere, the regional level, examining the impact on the development of the ring current in terms of energy density, anisotropy, and plasma distribution, and the local level to compare the new results to in-situ measurements of magnetic and electric field and plasma. The results will also be compared to past simulations using the one way coupling and no coupling whatsoever. This work is the first to fully couple an anisotropic kinetic ring current code with a selfconsistently calculated magnetic field to a set of global models.« less

Analysis of spectra of 3s-3p and 3p-3d transitions of highly-charged copper ions

NASA Astrophysics Data System (ADS)

Su, M. G.; Min, Q.; He, S. Q.; Wu, L.; Sun, R.; Ding, X. B.; Sun, D. X.

2017-08-01

Beam-foil excited spectra in the range of 160-360 Å from highly charged copper ions were identified with the aid of the National Institute of Standards and Technology Atomic Spectra Database and theoretical calculations with Cowan and Flexible Atomic Code (FAC) calculations. Spectra arising from 3s-3p and 3p-3d transitions of Cu13+-Cu22+ ions were considered. The ion fraction at an ion beam energy of 110 MeV was estimated from the equilibrium charge distribution of the fast ion beams after passing through the solid. The corresponding simulated spectra were in good agreement with the experimental result. Our Cowan and FAC calculation results should be useful for further spectral identification and lifetime measurements of highly charged copper ions.

On the limit of field-aligned current intensity in the polar magnetosphere

NASA Technical Reports Server (NTRS)

Cole, Keith D.

1991-01-01

Field-aligned current (FAC) is here defined by 4 pi j = alpha B, where alpha is constant along a magnetic field line. The upper limit value of alpha in the polar magnetosphere, possible source regions of the strongest FAC and the relationship of them to some auroral and ionospheric irregularity cross-field scale sizes are discussed. Cross-field dimensions of the strongest FAC are related to the gyroradii of source particles (O(+), He(2+), He(+), H(+), e) in the current-generating region. It is suggested that experimental determination, and mapping of the values of alpha, may assist with the search for the generators of such currents in near-earth space including in the nearby solar wind. The upper limit of alpha is associated with the breakup of FAC systems.

Alfvenic Generation of Field-Aligned Currents and Displacement Currents in the M-I Coupling System and the Formation of Discrete Auroral Arcs

NASA Astrophysics Data System (ADS)

Song, Y.; Lysak, R. L.

2016-12-01

In previous theories (e.g., Hasegawa and Sato, 1979; Sato and Iijima, 1979; Vasyliunas, 1984), field-aligned current (FAC) generation is derived from current continuity assumption plus the force balance between the Lorentz force and other forces in the MHD momentum equation. These theories suggest that the FAC is generated by other forces, such as the inertia and/or pressure gradients. In fact, the FAC cannot be generated by these forces. From Maxwell's equations, FAC generation is associated with enhanced sheared magnetic fields and free magnetic energy where a dynamo action and Alfven waves are needed to generate and transport free magnetic energy. It is obvious that the mechanism of FAC generation cannot be given by analyzing a local force balance. We propose that FACs are generated by Alfvenic interactions in the M-I coupling driven system. From a full set of the dynamical equations, we have found that the generation of the total FAC (J||total ) is associated with spatial gradients of the parallel vorticity, where J||total=J||+J||D, and J||D=(1/4∏)(∂E||/∂t) is the displacement current, which describes E|| generation (Song and Lysak, 2006). The J||total generation is a dynamo process associated with the increase of the azimuthal magnetic flux caused by the axial torque acting on FAC flux tubes. Although the magnitude of the J||D is often very small relative to J||, neglecting this term, we cannot find the mechanism of the E|| generation. When the plasma density is low J||D becomes important relative to the current. We will demonstrate how the generation of E|| and the formation of auroral arcs can redistribute perpendicular mechanical and magnetic stresses which can cause a sudden and violent tail energy release and enhance the total FAC leading to the substorm auroral poleward expansion. We will also show how the nonlinear interaction of incident and reflected Alfven wave packets in the auroral acceleration region can produce quasi-stationary non-propagating electromagnetic plasma structures, such as Alfvenic double layers. These structures will sustain the J||D and can constitute powerful high energy particle accelerators, where electromagnetic energy can be efficiently converted to the particle energy.

Field aligned current study during the solar declining- extreme minimum of 23 solar cycle

NASA Astrophysics Data System (ADS)

Nepolian, Jeni Victor; Kumar, Anil; C, Panneerselvam

Field Aligned Current (FAC) density study has been carried out during the solar declining phase from 2004 to 2006 of the 23rd solar cycle and the ambient terrestrial magnetic field of the extended minimum period of 2008 and 2009. We mainly depended on CHAMP satellite data (http://isdc.gfz-potsdam.de/) for computing the FAC density with backup of IGRF-10 model. The study indicates that, the FAC is controlled by quasi-viscous processes occurring at the flank of the earth’s magnetosphere. The dawn-dusk conventional pattern enhanced during disturbed days. The intensity of R1 current system is higher than the R2 current system. Detailed results will be discussed in the conference.

DMSP F7 observations of a substorm field-aligned current

NASA Technical Reports Server (NTRS)

Lopez, R. E.; Spence, H. E.; Meng, C.-I.

1991-01-01

Observations are described of a substorm field-aligned current (FAC) system traversed by the DMSP F7 spacecraft just after 0300 UT on April 25, 1985. It is shown that the substorm FAC portion of the current system was located equatorward of the boundary between open and closed field lines. The equatorward boundary of the substorm FAC into the magnetotail was mapped using the Tsyganenko (1987) model, showing that the boundary corresponds to 6.9 earth radii. The result is consistent with the suggestion of Akasofu (1972) and Lopez and Lui (1990) that the region of substorm initiation lies relatively close to the earth and the concept that an essential feature of substorms is the disruption and diversion of the near-earth current sheet.

Stellar and laboratory XUV/EUV line ratios in Fe XVIII and Fe XIX

DOE Office of Scientific and Technical Information (OSTI.GOV)

Traebert, E.; Beiersdorfer, P.; Clementson, J.

2012-05-25

A so-called XUV excess has been suspected with the relative fluxes of Fe XVIII and Fe XIX lines observed in the XUV and EUV ranges of the spectrum of the star Capella as observed by the Chandra spacecraft, even after correction for interstellar absorption. This excess becomes apparent in the comparison of the observations with simulations of stellar spectra obtained using collisional-radiative models that employ, for example, the Atomic Plasma Emission Code (APEC) or the Flexible Atomic Code (FAC). We have addressed this problem by laboratory studies using the Livermore electron beam ion trap (EBIT).

Generation of field-aligned currents and Alfven waves by 3D magnetic reconnection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ma, Z.W.; Lee, L.C.; Otto, A.

1995-07-01

The authors have carried out a three-dimensional compressible MHD simulation to study the generation of field-aligned currents (FAC`s) and Alfven waves by magnetic reconnection for locally antiparallel magnetic fields across the current sheet. Reconnection is triggered by a localized resistivity. The results indicate that both FAC`s and Alfven waves are generated by the three-dimensional reconnection process. Two pairs of FAC`s are generated on each side of current sheet. The polarities of the resulting FAC pair in the leading bulge region are opposite to those of a FAC pair in the trailing quasi-steady region. It is further found that a largemore » portion of the FAC`s ({approximately}40%) is located in the closed field line region. They examine the Walen relation between FAC and parallel vorticity and find that Alfven waves are generated and propagate away from the reconnection site. They discuss the relevance of the results to the observed Region 1 FAC`s at noon. 15 refs., 4 figs.« less

Flow Accelerated Corrosion of Carbon Steel in the Feedwater System of PWR Plants - Behaviour of Welds and Weld Assemblies

NASA Astrophysics Data System (ADS)

Mansour, C.; Pavageau, E. M.; Faucher, A.; Inada, F.; Yoneda, K.; Miller, C.; Bretelle, J.-L.

Flow Accelerated Corrosion (FAC) of carbon steel is a phenomenon that has been studied for many years. However, to date, the specific behavior of welds and weld assemblies of carbon steel towards this phenomenon has been scarcely examined. An experimental program of FAC of welds and weld assemblies is being conducted by EDF and CRIEPI. This paper describes the results obtained on the behavior of weld metal independently of its behavior in a weld assembly as well as the sensitivity to FAC of various weld assembly configurations. Tests are performed, at EDF, in the CIROCO loop which permits to follow the FAC rate by gammametry measurements, and at CRIEPI, in the PRINTEMPS loop where FAC is measured by laser displacement sensor. Welds are performed by two different methods: Submerged Arc Welding (SAW) and Gas Tungsten Arc Welding (GTAW). The influence of several parameters on FAC of welds is examined: welding method, chromium content and temperature. For weld assemblies, only the impact of chromium content is studied. All the tests are conducted in ammonia medium at pH 9.0 and oxygen concentration lower then 1 ppb. Chemical parameters, as the pH, the conductivity and oxygen concentration, are measured in situ during the test and surface characterizations are performed after the test. The results show that, with more than 0.15% chromium, no FAC is detected on the weld metal, which is similar to the base metal behaviour. For the same and lower chromium content, the two types of metal have the same FAC rate. Concerning the temperature effect, for both metals FAC rate decreases with temperature increase above 150°C. Below 150 °C, their behaviour seems to be different. For weld assemblies, the study of different configurations shows that the chromium content is the main parameter affecting the behaviour of the specimens. Additional tests and modeling studies will be conducted in order to complete the results.

Excitation rates for transitions in Kr XXXII

NASA Astrophysics Data System (ADS)

Aggarwal, K. M.; Keenan, F. P.; Lawson, K. D.

2009-04-01

In this paper we report our results for collision strengths and effective collision strengths for transitions among the lowest 125 levels of the 2s22p, 2s2p2, 2p3, 2s23ell, 2s2p3ell, and 2p23ell configurations of Kr XXXII. For our calculations both the FAC and DARC codes have been employed.

Mild and Low-Pressure fac-Ir(ppy)3 -Mediated Radical Aminocarbonylation of Unactivated Alkyl Iodides through Visible-Light Photoredox Catalysis.

PubMed

Chow, Shiao Y; Stevens, Marc Y; Åkerbladh, Linda; Bergman, Sara; Odell, Luke R

2016-06-27

A novel, mild and facile preparation of alkyl amides from unactivated alkyl iodides employing a fac-Ir(ppy)3 -catalyzed radical aminocarbonylation protocol has been developed. Using a two-chambered system, alkyl iodides, fac-Ir(ppy)3 , amines, reductants, and CO gas (released ex situ from Mo(CO)6 ), were combined and subjected to an initial radical reductive dehalogenation generating alkyl radicals, and a subsequent aminocarbonylation with amines affording a wide range of alkyl amides in moderate to excellent yields. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Status of Line identifications in the ChandraLETG Spectrum of Procyon

DOE Office of Scientific and Technical Information (OSTI.GOV)

Desai, P; Beiersdorfer, P; Brickhouse, N S

We report on our ongoing efforts to produce a relatively complete set of X-ray emission lines present in the Low Energy Transmission Grating (LETG) spectra of Procyon. In addition to using recently reprocessed HRC-S/LETG spectra of Procyon, we use LETG spectra of Capella, along with recent Electron Bean Ion Trap (EBIT) laboratory data and new Flexible Atomic Code (FAC) calculations to identify the wealth of weaker lines present in the spectrum. Experimental measurements at the EBIT-I and EBIT-II facilities at Livermore of astrophysically abundant elements (Fe VII-Fe X, Lepson et al. 2002, Ar IX-Ar XVI, Lepson et al 2003, Smore » VII, S XIV, Lepson et al 2004) and new FAC calculations of Mg and Ne (Gu, 2008 in press) provide an opportunity to identify many of the previously unassigned lines in Procyon (Raassen et al, 2002).« less

On the Spatio-Temporal Variability of Field-Aligned Currents Observed with the Swarm Satellite Constellation: Implications for the Energetics of Magnetosphere-Ionosphere Coupling

NASA Astrophysics Data System (ADS)

Pakhotin, I.; Mann, I. R.; Forsyth, C.; Rae, J.; Burchill, J. K.; Knudsen, D. J.; Murphy, K. R.; Gjerloev, J. W.; Ozeke, L.; Balasis, G.; Daglis, I. A.

2016-12-01

With the advent of the Swarm mission with its multi-satellite capacity, it became possible for the first time to make systematic close separation multi-satellite measurements of the magnetic fields associated with field-aligned currents (FACs) at a 50 Hz cadence using fluxgate magnetometers. Initial studies have revealed an even greater level of detail and complexity and spatio-temporal non-stationarity than previously understood. On inter-satellite separation scales of 10 seconds along-track and <120 km cross-track, the peak-to-peak magnitudes of the small scale and poorly correlated inter-spacecraft magnetic field fluctuations can reach tens to hundreds of nanoteslas. These magnitudes are directly comparable to those associated with larger scale magnetic perturbations such as the global scale Region 1 and 2 FAC systems characterised by Iijima and Potemra 40 years ago. We evaluate the impact of these smaller scale magnetic perturbations relative to the larger scale FAC systems statistically as a function of the total number of FAC crossings observed, and as a function of geomagnetic indices, spatial location, and season. Further case studies incorporating Swarm electric field measurements enable estimates of the Poynting flux associated with the small scale and non-stationary magnetic fields. We interpret the small scale structures as Alfvenic, suggesting that Alfven waves play a much larger and more energetically significant role in magnetosphere-ionosphere coupling than previously thought. We further examine what causes such high variability among low-Earth orbit FAC systems to be observed under some conditions but not in others.

Seasonal and Temporal Variations of Field-Aligned Currents and Ground Magnetic Deflections During Substorms

NASA Astrophysics Data System (ADS)

Forsyth, C.; Shortt, M.; Coxon, J. C.; Rae, I. J.; Freeman, M. P.; Kalmoni, N. M. E.; Jackman, C. M.; Anderson, B. J.; Milan, S. E.; Burrell, A. G.

2018-04-01

Field-aligned currents (FACs), also known as Birkeland currents, are the agents by which energy and momentum are transferred to the ionosphere from the magnetosphere and solar wind. This coupling is enhanced at substorm onset through the formation of the substorm current wedge. Using FAC data from the Active Magnetosphere and Planetary Electrodynamics Response Experiment and substorm expansion phase onsets identified using the Substorm Onsets and Phases from Indices of the Electrojet technique, we examine the Northern Hemisphere FACs in all local time sectors with respect to substorm onset and subdivided by season. Our results show that while there is a strong seasonal dependence on the underlying FACs, the increase in FACs following substorm onset only varies by 10% with season, with substorms increasing the hemispheric FACs by 420 kA on average. Over an hour prior to substorm onset, the dayside currents in the postnoon quadrant increase linearly, whereas the nightside currents show a linear increase starting 20-30 min before onset. After onset, the nightside Region 1, Region 2, and nonlocally closed currents and the SuperMAG AL (SML) index follow the Weimer (1994, https://doi.org/10.1029/93JA02721) model with the same time constants in each season. These results contrast earlier contradictory studies that indicate that substorms are either longer in the summer or decay faster in the summer. Our results imply that, on average, substorm FACs do not change with season but that their relative impact on the coupled magnetosphere-ionosphere system does due to the changes in the underlying currents.

Microfluidics microFACS for Life Detection

NASA Technical Reports Server (NTRS)

Platt, Donald W.; Hoover, Richard B.

2010-01-01

A prototype micro-scale Fluorescent Activated Cell Sorter (microFACS) for life detection has been built and is undergoing testing. A functional miniature microfluidics instrument with the ability to remotely distinguish live or dead bacterial cells from abiotic particulates in ice or permafrost of icy bodies of the solar system would be of fundamental value to NASA. The use of molecular probes to obtain the bio-signature of living or dead cells could answer the most fundamental question of Astrobiology: Does life exist beyond Earth? The live-dead fluorescent stains to be used in the microFACS instrument function only with biological cell walls. The detection of the cell membranes of living or dead bacteria (unlike PAH's and many other Biomarkers) would provide convincing evidence of present or past life. This miniature device rapidly examine large numbers of particulates from a polar ice or permafrost sample and distinguish living from dead bacteria cells and biological cells from mineral grains and abiotic particulates and sort the cells and particulates based on a staining system. Any sample found to exhibit fluorescence consistent with living cells could then be used in conjunction with a chiral labeled release experiment or video microscopy system to seek addition evidence for cellular metabolism or motility. Results of preliminary testing and calibration of the microFACS prototype instrument system with pure cultures and enrichment assemblages of microbial extremophiles will be reported.

Field-aligned currents associated with multiple arc systems

NASA Astrophysics Data System (ADS)

Wu, J.; Knudsen, D. J.; Gillies, D. M.; Donovan, E.; Burchill, J. K.

2016-12-01

It is often thought that auroral arcs are a direct consequence of upward field-aligned currents. In fact, the relation between currents and brightness is more complicated. Multiple auroral arc systems provide and opportunity to study this relation in detail; this information can be used as a test of models for quasi-static arc formation. In this study, we have identified two types of FAC configurations in multiple parallel arc systems using ground-based optical data from the THEMIS all-sky imagers (ASIs), magnetometers and electric field instruments onboard the Swarm satellites during the period from December 2013 to March 2015. In type 1 events, each arc is an intensification within a broad, unipolar current sheet and downward currents only exist outside the upward current sheet. In type 2 events, multiple arc systems represent a collection of multiple up/down current pairs. By collecting 12 events for type 1 and 17 events for type 2, we find that (1) Type 1 events are mainly located between 22-23MLT. Type 2 events are mainly located around midnight. (2) The typical size of upward and downward FAC in type 2 events are comparable, while upward FAC in type 1 events are larger than downward FAC. (3) Upward currents with more arcs embedded have larger intensities and widths. (4) There is no significant difference between the characteristic widths of multiple arcs and single arcs.

Comment on "Atomic structure calculations and identification of EUV and SXR spectral lines in Sr XXX" by A. Goyal, I. Khatri, S. Aggarwal, A.K. Singh, M. Mohan [J Quant Spectrosc Radiat Transf 2015;161:157

NASA Astrophysics Data System (ADS)

Aggarwal, Kanti M.

2015-11-01

Recently, Goyal et al. [1] reported energies and lifetimes (τ) for the lowest 113 levels of the 2s22p5, 2s2p6, 2s22p43ℓ, 2s2p53ℓ and 2p63ℓ configurations of F-like Sr XXX. For the calculations they adopted the multi-configuration Dirac-Fock (MCDF) and the flexible atomic code (FAC). Additionally, they also listed radiative rates (A- values), oscillator strengths (f- values) and line strengths (S- values) for four types of transitions, namely electric dipole (E1), electric quadrupole (E2), magnetic dipole (M1) and magnetic quadrupole (M2), but only from the ground to the higher excited levels. However, there are two clear anomalies in their reported data. Firstly, the f-values listed from FAC in their Tables 3-6 are larger than from MCDF by a factor of two, for all transitions. This is because they have blindly listed the output from FAC without realising that, unlike MCDF, FAC lists ωf where ω is the statistical weight, and happens to be exactly 2 in the present case. Secondly, their lifetime for level 2 (2s22p51/2 o 2P) is incorrect. This is because the dominant contributing transition for this level is 1-2 M1 for which A=3.25×106 s-1, listed (correctly) in their Table 5, and this leads to τ=3.08×10-7 s, and not 1.54×10-7 s, as listed in their Table 1.

Absolute dosimetry on a dynamically scanned sample for synchrotron radiotherapy using graphite calorimetry and ionization chambers

NASA Astrophysics Data System (ADS)

Lye, J. E.; Harty, P. D.; Butler, D. J.; Crosbie, J. C.; Livingstone, J.; Poole, C. M.; Ramanathan, G.; Wright, T.; Stevenson, A. W.

2016-06-01

The absolute dose delivered to a dynamically scanned sample in the Imaging and Medical Beamline (IMBL) on the Australian Synchrotron was measured with a graphite calorimeter anticipated to be established as a primary standard for synchrotron dosimetry. The calorimetry was compared to measurements using a free-air chamber (FAC), a PTW 31 014 Pinpoint ionization chamber, and a PTW 34 001 Roos ionization chamber. The IMBL beam height is limited to approximately 2 mm. To produce clinically useful beams of a few centimetres the beam must be scanned in the vertical direction. In practice it is the patient/detector that is scanned and the scanning velocity defines the dose that is delivered. The calorimeter, FAC, and Roos chamber measure the dose area product which is then converted to central axis dose with the scanned beam area derived from Monte Carlo (MC) simulations and film measurements. The Pinpoint chamber measures the central axis dose directly and does not require beam area measurements. The calorimeter and FAC measure dose from first principles. The calorimetry requires conversion of the measured absorbed dose to graphite to absorbed dose to water using MC calculations with the EGSnrc code. Air kerma measurements from the free air chamber were converted to absorbed dose to water using the AAPM TG-61 protocol. The two ionization chambers are secondary standards requiring calibration with kilovoltage x-ray tubes. The Roos and Pinpoint chambers were calibrated against the Australian primary standard for air kerma at the Australian Radiation Protection and Nuclear Safety Agency (ARPANSA). Agreement of order 2% or better was obtained between the calorimetry and ionization chambers. The FAC measured a dose 3-5% higher than the calorimetry, within the stated uncertainties.

Prospective randomized trial of 5-fluorouracil, doxorubicin, and cyclophosphamide (FAC) versus paclitaxel and FAC (TFAC) in patients with operable breast cancer: impact of taxane chemotherapy on locoregional control.

PubMed

Albert, Jeffrey M; Buzdar, Aman U; Guzman, Reina; Allen, Pamela K; Strom, Eric A; Perkins, George H; Woodward, Wendy A; Hoffman, Karen E; Tereffe, Welela; Hunt, Kelly K; Buchholz, Thomas A; Oh, Julia L

2011-07-01

A previous randomized trial (CALGB 9344/Intergroup 0148) compared four cycles of adjuvant doxorubicin/cyclophosphamide (AC) to four cycles of AC plus four cycles of paclitaxel (AC + T) and demonstrated that the addition of paclitaxel improved locoregional control (LRC) in patients with node-positive breast cancer. However, it could not be determined whether it was the paclitaxel or the increased duration of chemotherapy that led to this improvement. The present study aimed to analyze whether the addition of paclitaxel to a doxorubicin-based regimen improves LRC in a cohort of patients who all received eight total cycles of chemotherapy. Five hundred eleven women with operable breast cancer were randomized on a single-institution prospective trial to receive 5-fluorouracil, doxorubicin, cyclophosphamide (FAC) × 8 cycles (n = 252) or FAC × 4 cycles plus paclitaxel × 4 cycles (TFAC) (n = 259). Rates of LRC and overall survival (OS) were analyzed. Median follow-up was 124 months (range 5-167 months). The 10-year LRC rate was 92.6 versus 93.1% in the FAC versus TFAC arms, respectively (P = 0.26). The LRC between treatment arms did not differ when analyzed by locoregional treatment group: breast conservation therapy (BCT), mastectomy alone (M), and mastectomy + radiation (M + RT). The 10-year LRC rates were 95.1% (FAC) versus 91.2% (TFAC) after BCT (P = 0.98), 89.5% (FAC) versus 93.4% (TFAC) after M (P = 0.24), and 94.7% (FAC) versus 96.5% (TFAC) after M + RT (P = 0.59). Additionally, there was no difference in OS between the treatment arms, with 10-year OS rates of 78.4% (FAC) versus 81.7% (TFAC) (P = 0.93). The addition of paclitaxel to a doxorubicin-based regimen had no impact on LRC, regardless of the type of local therapy received. Historically inferior LRC with AC chemotherapy alone versus AC + T may have been due to an inadequate duration of systemic therapy and not due to the absence of paclitaxel.

Probing the mer- to fac-isomerization of tris-cyclometallated homo- and heteroleptic (C,N)3 iridium(III) complexes.

PubMed

McDonald, Aidan R; Lutz, Martin; von Chrzanowski, Lars S; van Klink, Gerard P M; Spek, Anthony L; van Koten, Gerard

2008-08-04

We have developed techniques which allow for covalent tethering, via a "hetero" cyclometallating ligand, of heteroleptic tris-cyclometallated iridium(III) complexes to polymeric supports (for application in light-emitting diode technologies). This involved the selective synthesis and thorough characterization of heteroleptic [Ir(C,N) 2(C',N')] tris-cyclometallated iridium(III) complexes. Furthermore, the synthesis and characterization of heteroleptic [Ir(C,N) 2OR] complexes is presented. Under standard thermal conditions for the synthesis of the facial ( fac) isomer of tris-cyclometallated complexes, it was not possible to synthesize pure heteroleptic complexes of the form [Ir(C,N) 2(C',N')]. Instead, a mixture of homo- and heteroleptic complexes was acquired. It was found that a stepwise procedure involving the synthesis of a pure meridonial ( mer) isomer followed by photochemical isomerization of this mer to the fac isomer was necessary to synthesize pure fac-[Ir(C,N) 2(C',N')] complexes. Under thermal isomerization conditions, the conversion of mer-[Ir(C,N) 2(C',N')] to fac-[Ir(C,N) 2(C',N')] was also not a clean reaction, with again a mixture of homo- and heteroleptic complexes acquired. An investigation into the thermal mer to fac isomerization of both homo- and heteroleptic tris-cyclometallated complexes is presented. It was found that the process is an alcohol-catalyzed reaction with the formation of an iridium alkoxide [Ir(C,N) 2OR] intermediate in the isomerization process. This catalyzed reaction can be carried out between 50 and 100 degrees C, the first such example of low-temperature mer-fac thermal isomerization. We have synthesized analogous complexes and have shown that they do indeed react so as to give fac-tris-cyclometallated products. A detailed explanation of the intermediates (and all of their stereoisomers, in particular when systems of the generic formula [M(a,b) 2(a',b')] are synthesized) formed in the mer to fac isomerization process is presented, including how the formed intermediates react further, and the stereoisomeric products they yield.

A Prospective Comparative Study of the Toxicity Profile of 5-Flurouracil, Adriamycin, Cyclophosphamide Regime VS Adriamycin, Paclitaxel Regime in Patients with Locally Advanced Breast Carcinoma

PubMed Central

Pillai, Pradeep Sadasivan; Jayakumar, Krishnan Nair Lalithamma

2015-01-01

Introduction A 5-flurouracil, Adriamycin, Cyclophosphamide (FAC) and Adriamycin, Paclitaxel (AT) are two popular chemotherapeutic regimens for treatment of breast carcinoma. The most time tested and popular regimen is FAC. It is extensively studied for efficacy and toxicity. But data regarding toxicity profile and efficacy of AT regimen is sparse. Aim To study the toxicity profile, severity of toxicities and clinical response rate of FAC and AT regimens in patients with locally advanced breast carcinoma. Materials and Methods A prospective observational study with 50 patients in each treatment arm. Study duration was 12 months from November 2012 to October 2013. Consecutive patients with locally advanced breast carcinoma receiving treatment with either FAC or AT regimen, satisfying inclusion criteria were enrolled into the study after getting informed written consent. Prior to initiation of treatment detailed medical history was taken from all patients. General clinical examination, examination of organ systems and local examination of breast lump were done. After each cycle of chemotherapy and after completion of treatment patients were interviewed and examined for clinical response and toxicities. Toxicities were graded with WHO toxicity grading criteria. All data were entered in a structured proforma. At least 50% reduction in tumour size was taken as adequate clinical response. Statistical Analysis Data was analysed using Chi-square test with help of Excel 2007 and SPSS-16 statistical software. Results Different pattern of toxicities were seen with FAC and AT regimens. Anaemia, thrombocytopenia, stomatitis, hyperpigmentation, photosensitivity and diarrhoea were more common with patients receiving FAC regimen. Leucopenia, peripheral neuropathy, myalgia, arthralgia, vomiting and injection site reactions were more common in AT regimen. Both FAC and AT regimens gave 100% clinical response. Conclusion FAC and AT regimens are equally efficacious but have different toxicity profiles. Patient’s predisposition to toxicities may govern the selection of a particular regime. PMID:26870703

Methodology and measures for preventing unacceptable flow-accelerated corrosion thinning of pipelines and equipment of NPP power generating units

NASA Astrophysics Data System (ADS)

Tomarov, G. V.; Shipkov, A. A.; Lovchev, V. N.; Gutsev, D. F.

2016-10-01

Problems of metal flow-accelerated corrosion (FAC) in the pipelines and equipment of the condensate- feeding and wet-steam paths of NPP power-generating units (PGU) are examined. Goals, objectives, and main principles of the methodology for the implementation of an integrated program of AO Concern Rosenergoatom for the prevention of unacceptable FAC thinning and for increasing operational flow-accelerated corrosion resistance of NPP EaP are worded (further the Program). A role is determined and potentialities are shown for the use of Russian software packages in the evaluation and prediction of FAC rate upon solving practical problems for the timely detection of unacceptable FAC thinning in the elements of pipelines and equipment (EaP) of the secondary circuit of NPP PGU. Information is given concerning the structure, properties, and functions of the software systems for plant personnel support in the monitoring and planning of the inservice inspection of FAC thinning elements of pipelines and equipment of the secondary circuit of NPP PGUs, which are created and implemented at some Russian NPPs equipped with VVER-1000, VVER-440, and BN-600 reactors. It is noted that one of the most important practical results of software packages for supporting NPP personnel concerning the issue of flow-accelerated corrosion consists in revealing elements under a hazard of intense local FAC thinning. Examples are given for successful practice at some Russian NPP concerning the use of software systems for supporting the personnel in early detection of secondary-circuit pipeline elements with FAC thinning close to an unacceptable level. Intermediate results of working on the Program are presented and new tasks set in 2012 as a part of the updated program are denoted. The prospects of the developed methods and tools in the scope of the Program measures at the stages of design and construction of NPP PGU are discussed. The main directions of the work on solving the problems of flow-accelerated corrosion of pipelines and equipment in Russian NPP PGU are defined.

Investigating the development of double-peak subauroral ion drift (DSAID)

NASA Astrophysics Data System (ADS)

Horvath, Ildiko; Lovell, Brian C.

2017-04-01

This study focuses on the newly described ionospheric feature, called double-peak subauroral ion drift (DSAID), which is a subclass of the well-known single-peak SAID. Double-layer Region 2 (R2) field aligned currents (FACs) could be the main driver of DSAID. Our aim is to gain new insights into the development of DSAID during its two-stage progression. Observational results are provided by five scenarios, each demonstrating a certain progression sequence of DSAID. Results show that SAID/DSAID occurred during flux transfer events and was accompanied by flow channels (FCs) associated with dayside magnetopause (FC-2) and nightside magnetotail (FC-3) reconnections, with westward electrojet (eastward FC), and with auroral streamers (FC-4). In the premidnight magnetic local time (MLT) sector of stage 2, DSAID development was due to the short-circuiting of the reconnection-injected plasma jets during substorms or pseudobreakups. Thus, the related ring current pressure buildup enhanced the downward R2 FACs leading to double/multiple circuits forming double-layer R2 FACs. During the midnight MLT hours of stage 2, DSAID development was closely related to the westward traveling surge (WTS)/substorm current wedge (SCW). WTS/SCW-related strong upward R1 FACs closed with meriodional currents producing eastward and downward (i.e., downward R2 FAC-style) return currents enhancing the downward R2 FACs and thus leading to double/multiple circuits forming double-layer R2 FACs. Auroral streamers/FC-4 represent a substorm substructure and their occurrence with DSAID after stage 2 demonstrates that this substructure occasionally includes DSAID. Our results demonstrate also that the short-circuited system underlying SAID/DSAID acted sometimes as a current generator and sometimes as a voltage generator.

2 CFR 200.36 - Federal Audit Clearinghouse (FAC).

Code of Federal Regulations, 2014 CFR

2014-01-01

... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false Federal Audit Clearinghouse (FAC). 200.36... Clearinghouse (FAC). FAC means the clearinghouse designated by OMB as the repository of record where non-Federal... part. The mailing address of the FAC is Federal Audit Clearinghouse, Bureau of the Census, 1201 E. 10th...

CFD Application to Flow-Accelerated Corrosion in Feeder Bends

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pietralik, John M.; Smith, Bruce A.W.

2006-07-01

Feeder piping in CANDU{sup R} plants experiences a thinning degradation mechanism called Flow-Accelerated Corrosion (FAC). The piping is made of carbon steel and has high water flow speeds. Although the water chemistry is highly alkaline with room-temperature pH in a range of 10.0-10.5, the piping has FAC rates exceeding 0.1 mm/year in some locations, e.g., in bends. One of the most important parameters affecting the FAC rate is the mass transfer coefficient for convective mass transport of ferrous ions. The ions are created at the pipe wall as a result of corrosion, diffuse through the oxide layer, and are transportedmore » from the oxide-layer/water interface to the bulk water by mass transport. Consequently, the local flow characteristics contribute to the highly turbulent convective mass transfer. Plant data and laboratory experiments indicate that the mass transfer step dominates FAC under feeder conditions. In this study, the flow and mass transfer in a feeder bend under operating conditions were simulated using the Fluent{sup TM} computer code. Because the flow speed is very high, with the Reynolds numbers in a range of several millions, and because the geometry is complex, experiments in a 1:1 scale were conducted with the main objective to validate flow simulations. The experiments measured pressure at several key locations and visualized the flow. The flow and mass transfer models were validated using available friction-factor and mass transfer correlations and literature experiments on mass transfer in a bend. The validation showed that the turbulence model that best predicts the experiments is the realizable k-{epsilon} model. Other two-equation turbulence models, as well as one-equation models and Reynolds stress models were tried. The near-wall treatment used the non-equilibrium wall functions. The wall functions were modified for surface roughness when necessary. A comparison of the local mass transfer coefficient with measured FAC rate in plant specimens shows very good agreement. Visualization experiments indicate secondary flows in the bends. No boundary layer separation was observed in experiments or in simulations. (authors)« less

Mathematical Modeling for Evaluation of Field Water Supply Alternatives (Arid and Semi-Arid Regions).

DTIC Science & Technology

1981-01-01

switch box would normally be required to operate the diatomite filter pumps. However, in hot arid regions where ambient water temperatures are re- latively...9 Tactical Water Distribution Systems...................... 9 Temperature of Waters in the Desert ..................... 10...concentration of FAC when loaded; daily FAC; daily water temperature in each tank; weekly pH determination; results of all chemical laboratory

Social Risk and Depression: Evidence from Manual and Automatic Facial Expression Analysis

PubMed Central

Girard, Jeffrey M.; Cohn, Jeffrey F.; Mahoor, Mohammad H.; Mavadati, Seyedmohammad; Rosenwald, Dean P.

2014-01-01

Investigated the relationship between change over time in severity of depression symptoms and facial expression. Depressed participants were followed over the course of treatment and video recorded during a series of clinical interviews. Facial expressions were analyzed from the video using both manual and automatic systems. Automatic and manual coding were highly consistent for FACS action units, and showed similar effects for change over time in depression severity. For both systems, when symptom severity was high, participants made more facial expressions associated with contempt, smiled less, and those smiles that occurred were more likely to be accompanied by facial actions associated with contempt. These results are consistent with the “social risk hypothesis” of depression. According to this hypothesis, when symptoms are severe, depressed participants withdraw from other people in order to protect themselves from anticipated rejection, scorn, and social exclusion. As their symptoms fade, participants send more signals indicating a willingness to affiliate. The finding that automatic facial expression analysis was both consistent with manual coding and produced the same pattern of depression effects suggests that automatic facial expression analysis may be ready for use in behavioral and clinical science. PMID:24598859

CellSort: a support vector machine tool for optimizing fluorescence-activated cell sorting and reducing experimental effort.

PubMed

Yu, Jessica S; Pertusi, Dante A; Adeniran, Adebola V; Tyo, Keith E J

2017-03-15

High throughput screening by fluorescence activated cell sorting (FACS) is a common task in protein engineering and directed evolution. It can also be a rate-limiting step if high false positive or negative rates necessitate multiple rounds of enrichment. Current FACS software requires the user to define sorting gates by intuition and is practically limited to two dimensions. In cases when multiple rounds of enrichment are required, the software cannot forecast the enrichment effort required. We have developed CellSort, a support vector machine (SVM) algorithm that identifies optimal sorting gates based on machine learning using positive and negative control populations. CellSort can take advantage of more than two dimensions to enhance the ability to distinguish between populations. We also present a Bayesian approach to predict the number of sorting rounds required to enrich a population from a given library size. This Bayesian approach allowed us to determine strategies for biasing the sorting gates in order to reduce the required number of enrichment rounds. This algorithm should be generally useful for improve sorting outcomes and reducing effort when using FACS. Source code available at http://tyolab.northwestern.edu/tools/ . k-tyo@northwestern.edu. Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com

Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening.

PubMed

Mazor, Yariv; Van Blarcom, Thomas; Carroll, Sean; Georgiou, George

2010-05-01

Phage display of antibody libraries is a powerful tool for antibody discovery and evolution. Recombinant antibodies have been displayed on phage particles as scFvs or Fabs, and more recently as bivalent F(ab')(2). We recently developed a technology (E-clonal) for screening of combinatorial IgG libraries using bacterial periplasmic display and selection by fluorescence-activated cell sorting (FACS) [Mazor Y et al. (2007) Nat Biotechnol 25, 563-565]. Although, as a single-cell analysis technique, FACS is very powerful, especially for the isolation of high-affinity binders, even with state of the art instrumentation the screening of libraries with diversity > 10(8) is technically challenging. We report here a system that takes advantage of display of full-length IgGs on filamentous phage particles as a prescreening step to reduce library size and enable subsequent rounds of FACS screening in Escherichia coli. For the establishment of an IgG phage display system, we utilized phagemid-encoded IgG with the fUSE5-ZZ phage as a helper phage. These phage particles display the Fc-binding ZZ protein on all copies of the phage p3 coat protein, and are exploited as both helper phages and anchoring surfaces for the soluble IgG. We demonstrate that tandem phage selection followed by FACS allows the selection of a highly diversified profile of binders from antibody libraries without undersampling, and at the same time capitalizes on the advantages of FACS for real-time monitoring and optimization of the screening process.

A novel radiochemical approach to 1-(2'-deoxy-2'-[(18) F]fluoro-β-d-arabinofuranosyl)cytosine ((18) F-FAC).

PubMed

Meyer, Jan-Philip; Probst, Katrin C; Trist, Iuni M L; McGuigan, Christopher; Westwell, Andrew D

2014-09-01

(18) F-FAC (1-(2'-deoxy-2'-[(18) F]fluoro-β-D-arabinofuranosyl)-cytosine) is an important 2'-fluoro-nucleoside-based positron emission tomography (PET) tracer that has been used for in vivo prediction of response to the widely used cancer chemotherapy drug gemcitabine. Previously reported synthetic routes to (18) F-FAC have relied on early introduction of the (18) F radiolabel prior to attachment to protected cytosine base. Considering the (18) F radiochemical half-life (110 min) and the technical challenges of multi-step syntheses on PET radiochemistry modular systems, late-stage radiofluorination is preferred for reproducible and reliable radiosynthesis with in vivo applications. Herein, we report the first late-stage radiosynthesis of (18) F-FAC. Cytidine derivatives with leaving groups at the 2'-position are particularly prone to undergo anhydro side-product formation upon heating because of their electron density at the 2-carbonyl pyrimidone oxygen. Our rationally developed fluorination precursor showed an improved reactivity-to-stability ratio at elevated temperatures. (18) F-FAC was obtained in radiochemical yields of 4.3-5.5% (n = 8, decay-corrected from end of bombardment), with purities ≥98% and specific activities ≥63 GBq/µmol. The synthesis time was 168 min. Copyright © 2014 John Wiley & Sons, Ltd.

Functional Activity of the Fanconi Anemia Protein FAA Requires FAC Binding and Nuclear Localization

PubMed Central

Näf, Dieter; Kupfer, Gary M.; Suliman, Ahmed; Lambert, Kathleen; D’Andrea, Alan D.

1998-01-01

Fanconi anemia (FA) is an autosomal recessive disease characterized by genomic instability, cancer susceptibility, and cellular hypersensitivity to DNA-cross-linking agents. Eight complementation groups of FA (FA-A through FA-H) have been identified. Two FA genes, corresponding to complementation groups FA-A and FA-C, have been cloned, but the functions of the encoded FAA and FAC proteins remain unknown. We have recently demonstrated that FAA and FAC interact to form a nuclear complex. In this study, we have analyzed a series of mutant forms of the FAA protein with respect to functional activity, FAC binding, and nuclear localization. Mutation or deletion of the amino-terminal nuclear localization signal (NLS) of FAA results in loss of functional activity, loss of FAC binding, and cytoplasmic retention of FAA. Replacement of the NLS sequence with a heterologous NLS sequence, derived from the simian virus 40 T antigen, results in nuclear localization but does not rescue functional activity or FAC binding. Nuclear localization of the FAA protein is therefore necessary but not sufficient for FAA function. Mutant forms of FAA which fail to bind to FAC also fail to promote the nuclear accumulation of FAC. In addition, wild-type FAC promotes the accumulation of wild-type FAA in the nucleus. Our results suggest that FAA and FAC perform a concerted function in the cell nucleus, required for the maintenance of chromosomal stability. PMID:9742112

Magnetospheric Multiscale Observations of Field-Aligned Currents in the Magnetotail

NASA Astrophysics Data System (ADS)

Strangeway, R. J.; Russell, C. T.; Zhao, C.; Plaschke, F.; Fischer, D.; Anderson, B. J.; Weygand, J. M.; Le, G.; Kepko, L.; Nakamura, R.; Baumjohann, W.; Slavin, J. A.; Paterson, W. R.; Giles, B. L.; Shuster, J. R.; Torbert, R. B.; Burch, J. L.

2017-12-01

Field-aligned currents (FACs) are frequently observed by Magnetospheric Multiscale (MMS) within the Earth's magnetotail. However, unlike the FACs observed by MMS at the dayside magnetopause, which are of the order 100s of nA/m2, the magnetotail FACs are relatively weak, of the order 10s of nA/m2. There appear to be a variety of sources for the FACs. FACs are observed in association with dipolarization fronts that are propagating both earthward and tailward, at the boundary of the current sheet, and in flux-ropes. FACs are also observed to be embedded in regions of high speed flow, both earthward and tailward, and not just at the dipolarization front frequently associated with high speed flows. As is the case for FACs observed at the dayside magnetopause, these observations raise questions as to how or where the FACs close.

Theoretical electron-impact-ionization cross section for Fe11+ forming Fe12+

NASA Astrophysics Data System (ADS)

Kwon, Duck-Hee; Savin, Daniel Wolf

2012-08-01

We have calculated cross sections for electron impact ionization (EII) of P-like Fe11+ forming Si-like Fe12+. We have used the flexible atomic code (FAC) and a distorted-wave (DW) approximation. Particular attention has been paid to the ionization through the 3l→nl' and 2l→nl' excitation autoionization (EA) channels. We compare our results to previously published FAC DW results and recent experimental results. We find that the previous discrepancy between theory and experiment at the EII threshold can be accounted for by the 3l→nl' EA channels which were not included in the earlier calculations. At higher energies the discrepancy previously seen between theory and experiment for the magnitude of the 2l→nl'(n≥4) EA remains, though the difference has been reduced by our newer results. The resulting Maxwellian rate coefficient derived from our calculations lies within 11% of the experimentally derived rate coefficient in the temperature range where Fe11+ forms in collisional ionization equilibrium.

KLL dielectronic recombination resonant strengths of He-like up to O-like xenon ions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yao, K.; Geng, Z.; Xiao, J.

2010-02-15

In this work, the KLL dielectronic recombination (DR) resonant strengths of He- through to O-like Xe ions were studied, both through experiment and calculation. The experiments were done using a fast electron beam-energy scanning technique at the Shanghai electron beam ion trap. The calculations were done by using the flexible atomic code (FAC), in which the relativistic configuration interaction (RCI) method was employed. For the total resonant strengths, the present experimental and theoretical results for He-, Be-, B-, C-, N-, and O-like Xe ions agree within experimental uncertainties (about 9%). But the experimental result for Li-like Xe is 14% highermore » than the calculation. The present FAC calculations of the total DR strengths were compared with the available previous calculations, using RCI or multiconfiguration Dirac-Fock (MCDF) methods, and the agreement was very good. In this work, some intermediate-state resolved KLL DR strengths were also obtained and compared with theoretical results, and more discrepancies were revealed.« less

77 FR 3751 - Commission Information Collection Activities (FERC-725A); Comment Request; Submitted for OMB Review

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-25

... Commission, 888 First Street NE., Washington, DC 20426. FOR FURTHER INFORMATION CONTACT: Ellen Brown may be... Additional recordkeeping beyond FAC-008-1 FAC-008-3 Applies to: and FAC-009-1 and FAC-009-1 R1 Generator... Generator owners None, this requirement is derived Retention period increased by 2 years. from R1 of FAC-008...

Chiral Separation of G-type Chemical Warfare Nerve Agents via Analytical Seupercritical Fluid Chromatography

DTIC Science & Technology

2014-01-01

UNCLASSIFIED b . ABSTRACT UNCLASSIFIED c. THIS PAGE UNCLASSIFIED UNLIMITED 8 19b. TELEPHONE NUMBER (include area code) 410-436-4412 Standard Form 298...plates (N), retention fac- tor ( k ), separation factor (α), and resolution (RS). 16 Parameters were used to verify both the enantioselectivity and the...time, tR, was determined by averaging the time to peak max- ima from subsequent injections. Calculation of k was carried out using the following

Fatty acid-amino acid conjugates are essential for systemic activation of salicylic acid-induced protein kinase and accumulation of jasmonic acid in Nicotiana attenuata.

PubMed

Hettenhausen, Christian; Heinrich, Maria; Baldwin, Ian T; Wu, Jianqiang

2014-11-28

Herbivory induces the activation of mitogen-activated protein kinases (MAPKs), the accumulation of jasmonates and defensive metabolites in damaged leaves and in distal undamaged leaves. Previous studies mainly focused on individual responses and a limited number of systemic leaves, and more research is needed for a better understanding of how different plant parts respond to herbivory. In the wild tobacco Nicotiana attenuata, FACs (fatty acid-amino acid conjugates) in Manduca sexta oral secretions (OS) are the major elicitors that induce herbivory-specific signaling but their role in systemic signaling is largely unknown. Here, we show that simulated herbivory (adding M. sexta OS to fresh wounds) dramatically increased SIPK (salicylic acid-induced protein kinase) activity and jasmonic acid (JA) levels in damaged leaves and in certain (but not all) undamaged systemic leaves, whereas wounding alone had no detectable systemic effects; importantly, FACs and wounding are both required for activating these systemic responses. In contrast to the activation of SIPK and elevation of JA in specific systemic leaves, increases in the activity of an important anti-herbivore defense, trypsin proteinase inhibitor (TPI), were observed in all systemic leaves after simulated herbivory, suggesting that systemic TPI induction does not require SIPK activation and JA increases. Leaf ablation experiments demonstrated that within 10 minutes after simulated herbivory, a signal (or signals) was produced and transported out of the treated leaves, and subsequently activated systemic responses. Our results reveal that N. attenuata specifically recognizes herbivore-derived FACs in damaged leaves and rapidly send out a long-distance signal to phylotactically connected leaves to activate MAPK and JA signaling, and we propose that FACs that penetrated into wounds rapidly induce the production of another long-distance signal(s) which travels to all systemic leaves and activates TPI defense.

The National Wetland Plant List

DTIC Science & Technology

2012-10-01

P. Adams FACW Florida Sands St. John’s-Wort Hypericum fasciculatum Lam. FACW FACW FACW Peel -Bark St. John’s-Wort Hypericum fraseri...Dewflower Musa X paradisiaca L. (pro sp.) UPL FAC FACU Musa acuminata Colla FACW Edible Banana Musa troglodytarum L. FACU Fe’i... Banana Myoporum laetum G. Forst. FACU UPL Ngaio-Tree Myosotis arvensis (L.) Hill FAC FAC UPL FAC FAC FACU FACU Rough Forget-Me-Not

Significance of Cytochrome P450 System Responses and Levels of Bile Fluorescent Aromatic Compounds in Marine Wildlife Following Oil Spills

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Richard F.; Anderson, Jack W.

2005-07-01

The relationships among cytochrome P450 induction in marine wildlife species, levels of fluorescent aromatic compounds (FAC) in their bile, the chemical composition of the inducing compounds, the significance of the exposure pathway, and any resulting injury, as a consequence of exposure to crude oil following a spill, are reviewed. Fish collected after oil spills often show increases in cytochrome P450 system activity, cytochrome P4501A (CYP1A) and bile fluorescent aromatic compounds (FAC), that are correlated with exposure to polycyclic aromatic hydrocarbons (PAH) in the oil. There is also some evidence for increases in bile FAC and induction of cytochrome P450 inmore » marine birds and mammals after oil spills. However, when observed, increases in these exposure indicators are transitory and generally decrease to background levels within one year after the exposure. Laboratory studies have shown induction of cytochrome P450 systems occurs after exposure of fish to crude oil in water, sediment or food. Most of the PAH found in crude oil (dominantly 2- and 3-ring PAH) are not strong inducers of cytochrome P450. Exposure to the 4-ring chrysenes or the photooxidized products of the PAH may account for the cytochrome P450 responses in fish collected from oil-spill sites. The contribution of non-spill background PAH, particularly combustion-derived (pyrogenic) PAH, to bile FAC and cytochrome P450 system responses can be confounding and needs to be considered when evaluating oil spill effects. The ubiquity of pyrogenic PAH makes it important to fully characterize all sources of PAH, including PAH from natural resources, e.g. retene, in oil spill studies. In addition, such parameters as species, sex, age, ambient temperature and season need to be taken into account. While increases in fish bile FAC and cytochrome P450 system responses, can together, be sensitive general indicators of PAH exposure after an oil spill, there is little unequivocal evidence to suggest a linkage to higher order biological effects, e.g. toxicity, lesions, reproductive failure.« less

27 August 2001 substorm: Preonset phenomena, two main onsets, field-aligned current systems, and plasma flow channels in the ionosphere and in the magnetosphere

NASA Astrophysics Data System (ADS)

Mishin, V. M.; Mishin, V. V.; Lunyushkin, S. B.; Wang, J. Y.; Moiseev, A. V.

2017-05-01

We supplement the results of the 27 August 2001 substorm studied earlier in the series of papers. Described is the plasma flow in the nightside ionosphere from the near-polar region from the polar cap to the auroral oval during the substorm preonset phase and two expansion onsets, EO1 and EO2, produced by reconnection in the closed tail (magnetic reconnection (MR1) and in the open tail lobes (MR2), respectively. We discuss the location of the MR2 region (is it near, middle, and/or distant tail?) and the EO2 trigger mechanism. The upward substorm current wedge field-aligned current (FAC) and the downward FAC in the polar cap dusk sector that were both produced by different types of magnetosphere-ionosphere feedback instability are found to provide the main contribution to the system of FACs during EO1 and EO2. Also, we obtain the estimates for the EO1 and EO2 power and energy. Addressed are the variations in the tail lobe magnetic flux and their (variations) association with EO2. In addition, we describe a 3-D system of mesoscale cells, each of which involves a plasma vortex and a local FAC maximum. The cells of this system in the inner magnetosphere and in the tail lobes intensify one after other within 2 min interval. At last, we substantiate the assumption that the fast plasma flow recorded by the Cluster satellites 7 min prior to EO1 was a bursty bulk flow from the most distant tail.

Spontaneous and posed facial expression in Parkinson's disease.

PubMed

Smith, M C; Smith, M K; Ellgring, H

1996-09-01

Spontaneous and posed emotional facial expressions in individuals with Parkinson's disease (PD, n = 12) were compared with those of healthy age-matched controls (n = 12). The intensity and amount of facial expression in PD patients were expected to be reduced for spontaneous but not posed expressions. Emotional stimuli were video clips selected from films, 2-5 min in duration, designed to elicit feelings of happiness, sadness, fear, disgust, or anger. Facial movements were coded using Ekman and Friesen's (1978) Facial Action Coding System (FACS). In addition, participants rated their emotional experience on 9-point Likert scales. The PD group showed significantly less overall facial reactivity than did controls when viewing the films. The predicted Group X Condition (spontaneous vs. posed) interaction effect on smile intensity was found when PD participants with more severe disease were compared with those with milder disease and with controls. In contrast, ratings of emotional experience were similar for both groups. Depression was positively associated with emotion rating but not with measures of facial activity. Spontaneous facial expression appears to be selectively affected in PD, whereas posed expression and emotional experience remain relatively intact.

Assessment of Fever Advisory Cards (FACs) as an Initiative to Improve Febrile Neutropenia Management in a Regional Cancer Center Emergency Department.

PubMed

Kapil, Priyanka; MacMillan, Meghan; Carvalho, Maritza; Lymburner, Patricia; Fung, Ron; Almeida, Bernadette; Van Dorn, Laurie; Enright, Katherine

2016-09-01

We aimed to improve the time to antibiotics (TTA) for patients treated with chemotherapy who present to the emergency department (ED) with febrile neutropenia (FN) by using standardized fever advisory cards (FACs). Patients treated with chemotherapy who visited the ED at the Peel Regional Cancer Center in Ontario, Canada, with suspected FN were identified, before (April 2012 to March 2013) and after (October 2013 to March 2014) FAC implementation. The primary outcome of interest was TTA. Additional process measures included Canadian Triage and Acuity Scale score, time to physician assessment, and FAC compliance. Outcomes were analyzed with descriptive statistics and control charts to determine whether the change in primary measures were within statistical control over time. Between the pre-FAC cohort (n = 239) and post-FAC cohort (n = 69), TTA did not change significantly post-FACs (195 v 244 min, P = .09), with monthly averages demonstrating normal variation by statistical process control methodology. The introduction of FACs increased the percentage of patients with correctly assigned Canadian Triage and Acuity Scale scores (87% v 100%) but did not affect time to physician assessment. Compliance with FACs among patients was not ideal, with only 62.5% using them as intended. The distribution of FACs was associated with an improved incidence of correct FN triaging but did not demonstrate a meaningful improvement in the quality of FN management. This may be explained by FAC use among patients not being ideal. Next steps in the continued effort toward high-quality FN care include redesign of FACs, reinforcement of provider and patient education, and ED outreach. Copyright © 2016 by American Society of Clinical Oncology.

Functionalized granular activated carbon and surface complexation with chromates and bi-chromates in wastewater.

PubMed

Singha, Somdutta; Sarkar, Ujjaini; Luharuka, Pallavi

2013-03-01

Cr(VI) is present in the aqueous medium as chromate (CrO4(2-)) and bi-chromate (HCrO4(-)). Functionalized granular activated carbons (FACs) are used as adsorbents in the treatment of wastewaters containing hexavalent chromium. The FACs are prepared by chemical modifications of granular activated carbons (GACs) using functionalizing agents like HNO3, HCl and HF. The Brunauer, Emmett and Teller surface areas of FAC-HCl (693.5m(2)/g), FAC-HNO3 (648.8m(2)/g) and FAC-HF (726.2m(2)/g) are comparable to the GAC (777.7m(2)/g). But, the adsorption capacity of each of the FAC-HNO3, FAC-HCl and FAC-HF is found to be higher than the GAC. The functional groups play an important role in the adsorption process and pH has practically no role in this specific case. The FACs have hydrophilic protonated external surfaces in particular, along with the functional surface sites capable to make complexes with the CrO4(2-) and HCrO4(-) present. Surface complex formation is maximized in the order FAC-HNO3>FAC-HF>FAC-HCl, in proportion to the total surface acidity. This is also confirmed by the well-known pseudo second-order kinetic model. Physi-sorption equilibrium isotherms are parameterized by using standard Freundlich and Langmuir models. Langmuir fits better. The formation of surface complexes with the functional groups and hexavalent chromium is also revealed in the images of field emission scanning electron micrograph; energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy analysis after adsorption. The intra-particle diffusion is not the only rate-controlling factor. The Boyd's film diffusion model fits very well with R(2) as high as 98.1% for FAC-HNO3. This result demonstrates that the functionalization of the GAC by acid treatments would increase the diffusion rate, predominantly with a boundary layer diffusion effect. Copyright © 2013 Elsevier B.V. All rights reserved.

Fluorouracil, doxorubicin, and cyclophosphamide (FAC) versus FAC followed by weekly paclitaxel as adjuvant therapy for high-risk, node-negative breast cancer: results from the GEICAM/2003-02 study.

PubMed

Martín, Miguel; Ruiz, Amparo; Ruiz Borrego, Manuel; Barnadas, Agustí; González, Sonia; Calvo, Lourdes; Margelí Vila, Mireia; Antón, Antonio; Rodríguez-Lescure, Alvaro; Seguí-Palmer, Miguel Angel; Muñoz-Mateu, Montserrat; Dorca Ribugent, Joan; López-Vega, José Manuel; Jara, Carlos; Espinosa, Enrique; Mendiola Fernández, César; Andrés, Raquel; Ribelles, Nuria; Plazaola, Arrate; Sánchez-Rovira, Pedro; Salvador Bofill, Javier; Crespo, Carmen; Carabantes, Francisco J; Servitja, Sonia; Chacón, José Ignacio; Rodríguez, César A; Hernando, Blanca; Álvarez, Isabel; Carrasco, Eva; Lluch, Ana

2013-07-10

Adding taxanes to anthracycline-based adjuvant therapy improves survival outcomes of patients with node-positive breast cancer (BC). Currently, however, most patients with BC are node negative at diagnosis. The only pure node-negative study (Spanish Breast Cancer Research Group 9805) reported so far showed a docetaxel benefit but significant toxicity. Here we tested the efficacy and safety of weekly paclitaxel (wP) in node-negative patients, which is yet to be established. Patients with BC having T1-T3/N0 tumors and at least one high-risk factor for recurrence (according to St. Gallen 1998 criteria) were eligible. After primary surgery, 1,925 patients were randomly assigned to receive fluorouracil, doxorubicin, and cyclophosphamide (FAC) × 6 or FAC × 4 followed by wP × 8 (FAC-wP). The primary end point was disease-free survival (DFS) after a median follow-up of 5 years. Secondary end points included toxicity and overall survival. After a median follow-up of 63.3 months, 93% and 90.3% of patients receiving FAC-wP or FAC regimens, respectively, remained disease free (hazard ratio [HR], 0.73; 95% CI, 0.54 to 0.99; log-rank P = .04). Thirty-one patients receiving FAC-wP versus 40 patients receiving FAC died (one and seven from cardiovascular diseases, respectively; HR, 0.79; 95% CI, 0.49 to 1.26; log-rank P = .31). The most relevant grade 3 and 4 adverse events in the FAC-wP versus the FAC arm were febrile neutropenia (2.7% v 3.6%), fatigue (7.9% v 3.4%), and sensory neuropathy (5.5% v 0%). For patients with high-risk node-negative BC, the adjuvant FAC-wP regimen was associated with a small but significant improvement in DFS compared with FAC therapy, in addition to manageable toxicity, especially regarding long-term cardiac effects.

Facial correlates of emotional behaviour in the domestic cat (Felis catus).

PubMed

Bennett, Valerie; Gourkow, Nadine; Mills, Daniel S

2017-08-01

Leyhausen's (1979) work on cat behaviour and facial expressions associated with offensive and defensive behaviour is widely embraced as the standard for interpretation of agonistic behaviour in this species. However, it is a largely anecdotal description that can be easily misunderstood. Recently a facial action coding system has been developed for cats (CatFACS), similar to that used for objectively coding human facial expressions. This study reports on the use of this system to describe the relationship between behaviour and facial expressions of cats in confinement contexts without and with human interaction, in order to generate hypotheses about the relationship between these expressions and underlying emotional state. Video recordings taken of 29 cats resident in a Canadian animal shelter were analysed using 1-0 sampling of 275 4-s video clips. Observations under the two conditions were analysed descriptively using hierarchical cluster analysis for binomial data and indicated that in both situations, about half of the data clustered into three groups. An argument is presented that these largely reflect states based on varying degrees of relaxed engagement, fear and frustration. Facial actions associated with fear included blinking and half-blinking and a left head and gaze bias at lower intensities. Facial actions consistently associated with frustration included hissing, nose-licking, dropping of the jaw, the raising of the upper lip, nose wrinkling, lower lip depression, parting of the lips, mouth stretching, vocalisation and showing of the tongue. Relaxed engagement appeared to be associated with a right gaze and head turn bias. The results also indicate potential qualitative changes associated with differences in intensity in emotional expression following human intervention. The results were also compared to the classic description of "offensive and defensive moods" in cats (Leyhausen, 1979) and previous work by Gourkow et al. (2014a) on behavioural styles in cats in order to assess if these observations had replicable features noted by others. This revealed evidence of convergent validity between the methods However, the use of CatFACS revealed elements relating to vocalisation and response lateralisation, not previously reported in this literature. Copyright © 2017 Elsevier B.V. All rights reserved.

48 CFR 301.607-72 - Applicability.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Applicability. (a) The FAC-P/PM certification prerequisites and continuous learning requirements apply to all HHS employees who seek to obtain a FAC-P/PM certification. Although obtaining a FAC-P/PM certification... construction capital investment acquisitions. Consistent with OFPP guidance, HHS requires FAC-P/PM Level III...

48 CFR 301.607-72 - Applicability.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Applicability. (a) The FAC-P/PM certification prerequisites and continuous learning requirements apply to all HHS employees who seek to obtain a FAC-P/PM certification. Although obtaining a FAC-P/PM certification... construction capital investment acquisitions. Consistent with OFPP guidance, HHS requires FAC-P/PM Level III...

48 CFR 301.607-72 - Applicability.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Applicability. (a) The FAC-P/PM certification prerequisites and continuous learning requirements apply to all HHS employees who seek to obtain a FAC-P/PM certification. Although obtaining a FAC-P/PM certification... construction capital investment acquisitions. Consistent with OFPP guidance, HHS requires FAC-P/PM Level III...

Anger responses to psychosocial stress predict heart rate and cortisol stress responses in men but not women.

PubMed

Lupis, Sarah B; Lerman, Michelle; Wolf, Jutta M

2014-11-01

While previous research has suggested that anger and fear responses to stress are linked to distinct sympathetic nervous system (SNS) stress responses, little is known about how these emotions predict hypothalamus-pituitary-adrenal (HPA) axis reactivity. Further, earlier research primarily relied on retrospective self-report of emotion. The current study aimed at addressing both issues in male and female individuals by assessing the role of anger and fear in predicting heart rate and cortisol stress responses using both self-report and facial coding analysis to assess emotion responses. We exposed 32 healthy students (18 female; 19.6±1.7 yr) to an acute psychosocial stress paradigm (TSST) and measured heart rate and salivary cortisol levels throughout the protocol. Anger and fear before and after stress exposure was assessed by self-report, and video recordings of the TSST were assessed by a certified facial coder to determine emotion expression (FACS). Self-reported emotions and emotion expressions did not correlate (all p>.23). Increases in self-reported fear predicted blunted cortisol responses in men (β=0.41, p=.04). Also for men, longer durations of anger expression predicted exaggerated cortisol responses (β=0.67 p=.004), and more anger incidences predicted exaggerated cortisol and heart rate responses (β=0.51, p=.033; β=0.46, p=.066, resp.). Anger and fear did not predict SNS or HPA activity for females (all p>.23). The current differential self-report and facial coding findings support the use of multiple modes of emotion assessment. Particularly, FACS but not self-report revealed a robust anger-stress association that could have important downstream health effects for men. For women, future research may clarify the role of other emotions, such as self-conscious expressions of shame, for physiological stress responses. A better understanding of the emotion-stress link may contribute to behavioral interventions targeting health-promoting ways of responding emotionally to stress. Copyright © 2014 Elsevier Ltd. All rights reserved.

Anger responses to psychosocial stress predict heart rate and cortisol stress responses in men but not women

PubMed Central

Lupis, Sarah B.; Lerman, Michelle; Wolf, Jutta M.

2014-01-01

While previous research has suggested that anger and fear responses to stress are linked to distinct sympathetic nervous system (SNS) stress responses, little is known about how these emotions predict hypothalamus-pituitary-adrenal (HPA) axis reactivity. Further, earlier research primarily relied on retrospective self-report of emotion. The current study aimed at addressing both issues in male and female individuals by assessing the role of anger and fear in predicting heart rate and cortisol stress responses using both self-report and facial coding analysis to assess emotion responses. We exposed 32 healthy students (18 female; 19.6+/−1.7 yrs.) to an acute psychosocial stress paradigm (TSST) and measured heart rate and salivary cortisol levels throughout the protocol. Anger and fear before and after stress exposure was assessed by self-report, and video recordings of the TSST were assessed by a certified facial coder to determine emotion expression (FACS). Self-reported emotions and emotion expressions did not correlate (all p > .23). Increases in self-reported fear predicted blunted cortisol responses in men (β = 0.41, p = .04). Also for men, longer durations of anger expression predicted exaggerated cortisol responses (β = 0.67 p = .004), and more anger incidences predicted exaggerated cortisol and heart rate responses (β = 0.51, p = .033; β = 0.46, p = .066, resp.). Anger and fear did not predict SNS or HPA activity for females (all p > .23). The current differential self-report and facial coding findings support the use of multiple modes of emotion assessment. Particularly, FACS but not self-report revealed a robust anger-stress association that could have important downstream health effects for men. For women, future research may clarify the role of other emotions, such as self-conscious expressions of shame, for physiological stress responses. A better understanding of the emotion-stress link may contribute to behavioral interventions targeting health-promoting ways of responding emotionally to stress. PMID:25064831

Flow cytometry for enrichment and titration in massively parallel DNA sequencing

PubMed Central

Sandberg, Julia; Ståhl, Patrik L.; Ahmadian, Afshin; Bjursell, Magnus K.; Lundeberg, Joakim

2009-01-01

Massively parallel DNA sequencing is revolutionizing genomics research throughout the life sciences. However, the reagent costs and labor requirements in current sequencing protocols are still substantial, although improvements are continuously being made. Here, we demonstrate an effective alternative to existing sample titration protocols for the Roche/454 system using Fluorescence Activated Cell Sorting (FACS) technology to determine the optimal DNA-to-bead ratio prior to large-scale sequencing. Our method, which eliminates the need for the costly pilot sequencing of samples during titration is capable of rapidly providing accurate DNA-to-bead ratios that are not biased by the quantification and sedimentation steps included in current protocols. Moreover, we demonstrate that FACS sorting can be readily used to highly enrich fractions of beads carrying template DNA, with near total elimination of empty beads and no downstream sacrifice of DNA sequencing quality. Automated enrichment by FACS is a simple approach to obtain pure samples for bead-based sequencing systems, and offers an efficient, low-cost alternative to current enrichment protocols. PMID:19304748

Spectroscopy of M-shell x-ray transitions in Zn-like through Co-like W

DOE Office of Scientific and Technical Information (OSTI.GOV)

Clementson, J; Beiersdorfer, P; Brown, G V

2009-07-08

The M-shell x-ray emission of highly charged tungsten ions has been investigated at the Livermore electron beam ion trap facility. Using the SuperEBIT electron beam ion trap and a NASA x-ray calorimeter array, transitions connecting the ground configurations in the 1500-3600 eV spectral range of zinc-like W{sup 44+} through cobalt-like W{sup 47+} have been measured. The measured spectra are compared with theoretical line positions and emissivities calculated using the FAC code.

78 FR 13763 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-66; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-28

... Regulations Council (Councils) in this Federal Acquisition Circular (FAC) 2005-66. A companion document, the Small Entity Compliance Guide (SECG), follows this FAC. The FAC, including the SECG, is available via... in relation to each FAR case. Please cite FAC 2005-66 and the specific FAR case numbers. For...

A green emissive amorphous fac-Alq3 solid generated by grinding crystalline blue fac-Alq3 powder.

PubMed

Bi, Hai; Chen, Dong; Li, Di; Yuan, Yang; Xia, Dandan; Zhang, Zuolun; Zhang, Hongyu; Wang, Yue

2011-04-14

A novel green emissive Alq(3) solid with a facial isomeric form has been obtained by grinding the typical blue luminescent fac-Alq(3) crystalline powder. This is the first report, to the best of our knowledge, that a fac-Alq(3) isomer emits green light.

48 CFR 301.603-74 - Requirement for retention of FAC-C and HHS SAC certification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... of FAC-C and HHS SAC certification. 301.603-74 Section 301.603-74 Federal Acquisition Regulations..., Contracting Authority, and Responsibilities 301.603-74 Requirement for retention of FAC-C and HHS SAC certification. To maintain FAC-C certification, all warranted Contracting Officers, regardless of series, as...

75 FR 39413 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-44; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-08

... Acquisition Council and the Defense Acquisition Regulations Council in this Federal Acquisition Circular (FAC) 2005-44. A companion document, the Small Entity Compliance Guide (SECG), follows this FAC. The FAC.... Please cite FAC 2005-44 and the FAR case number. Interested parties may also visit our Web site at http...

77 FR 69713 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-62; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-20

... Regulations Council (Councils) in this Federal Acquisition Circular (FAC) 2005-62. A companion document, the Small Entity Compliance Guide (SECG), follows this FAC. The FAC, including the SECG, is available via... in relation to each FAR case. Please cite FAC 2005-62 and the specific FAR case numbers. For...

48 CFR 301.603-74 - Requirement for retention of FAC-C and HHS SAC certification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... of FAC-C and HHS SAC certification. 301.603-74 Section 301.603-74 Federal Acquisition Regulations..., Contracting Authority, and Responsibilities 301.603-74 Requirement for retention of FAC-C and HHS SAC certification. To maintain FAC-C certification, all warranted Contracting Officers, regardless of series, as...

76 FR 4187 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-49; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

..., GSA, and NASA in this Federal Acquisition Circular (FAC) 2005-49. A companion document, the Small Entity Compliance Guide (SECG), follows this FAC. The FAC, including the SECG, is available via the... FAR case. Please cite FAC 2005-49 and the specific FAR case number. For information pertaining to...

77 FR 75765 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-64; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-21

... (Councils) in this Federal Acquisition Circular (FAC) 2005-64. A companion document, the Small Entity Compliance Guide (SECG), follows this FAC. The FAC, including the SECG, is available via the Internet at http... cite FAC 2005-64 and the specific FAR case number. For information pertaining to status or publication...

48 CFR 301.603-74 - Requirement for retention of FAC-C and HHS SAC certification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... of FAC-C and HHS SAC certification. 301.603-74 Section 301.603-74 Federal Acquisition Regulations..., Contracting Authority, and Responsibilities 301.603-74 Requirement for retention of FAC-C and HHS SAC certification. To maintain FAC-C certification, all warranted Contracting Officers, regardless of series, as...

48 CFR 301.603-74 - Requirement for retention of FAC-C and HHS SAC certification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... of FAC-C and HHS SAC certification. 301.603-74 Section 301.603-74 Federal Acquisition Regulations..., Contracting Authority, and Responsibilities 301.603-74 Requirement for retention of FAC-C and HHS SAC certification. To maintain FAC-C certification, all warranted Contracting Officers, regardless of series, as...

77 FR 23363 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-58; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-18

...) in this Federal Acquisition Circular (FAC) 2005-58. A companion document, the Small Entity Compliance Guide (SECG), follows this FAC. The FAC, including the SECG, is available via the Internet at http://www... case. Please cite FAC 2005-58 and the specific FAR case numbers. For information pertaining to status...

Properties and behaviour of FAC currents in the inner magnetosphere

NASA Astrophysics Data System (ADS)

Yang, Junying; Dunlop, Malcolm; Yang, Yanyan; Xiong, Chao; Lühr, Hermann; Cao, Jinbin; Li, Liuyuan; Ma, Yuduan; Shen, Chao

2017-04-01

Cusp, region 1 and 2, and other large scale field-aligned currents (FACs), are sampled in situ by both the four Cluster spacecraft and by the three Swarm spacecraft at different altitudes, separated by a few to several Earth radii, and sometimes simultaneously. Here, the capability of Swarm-Cluster coordination for probing the behaviour of the field aligned currents (FACs) at medium and low orbits is explored. Joint signatures of R1 and R2 FACs (as well as cusp, R0 and NBZ currents) can be found and compared in terms of the magnetic signatures, using multi-spacecraft analysis where possible. Using the Swarm configuration, statistical correlation analysis of the local time variation of R1/R2 FACs can be shown and compared to standard MVA analysis. For context, we identify the associated auroral boundaries through application of a method to determine the FAC intensity gradients in order to interpret and resolve the R1 and R2 FACs. We also explore the relation of R2 FACs to the ring current properties measured in situ.

Evaluation of a 30-gene paclitaxel, fluorouracil, doxorubicin and cyclophosphamide chemotherapy response predictor in a multicenter randomized trial in breast cancer

PubMed Central

Tabchy, Adel; Valero, Vicente; Vidaurre, Tatiana; Lluch, Ana; Gomez, Henry; Martin, Miguel; Qi, Yuan; Barajas-Figueroa, Luis Javier; Souchon, Eduardo; Coutant, Charles; Doimi, Franco D; Ibrahim, Nuhad K; Gong, Yun; Hortobagyi, Gabriel N; Hess, Kenneth R; Symmans, W Fraser; Pusztai, Lajos

2010-01-01

Purpose We examined in a prospective, randomized, international clinical trial the performance of a previously defined 30-gene predictor (DLDA-30) of pathologic complete response (pCR) to preoperative weekly paclitaxel and fluorouracil, doxorubicin, cyclophosphamide (T/FAC) chemotherapy, and assessed if DLDA-30 also predicts increased sensitivity to FAC-only chemotherapy. We compared the pCR rates after T/FAC versus FAC×6 preoperative chemotherapy. We also performed an exploratory analysis to identify novel candidate genes that differentially predict response in the two treatment arms. Experimental Design 273 patients were randomly assigned to receive either weekly paclitaxel × 12 followed by FAC × 4 (T/FAC, n=138), or FAC × 6 (n=135) neoadjuvant chemotherapy. All patients underwent a pretreatment FNA biopsy of the tumor for gene expression profiling and treatment response prediction. Results The pCR rates were 19% and 9% in the T/FAC and FAC arms, respectively (p<0.05). In the T/FAC arm, the positive predictive value (PPV) of the genomic predictor was 38% (95%CI:21–56%), the negative predictive value (NPV) 88% (CI:77–95%) and the AUC 0.711. In the FAC arm, the PPV was 9% (CI:1–29%) and the AUC 0.584. This suggests that the genomic predictor may have regimen-specificity. Its performance was similar to a clinical variable-based predictor nomogram. Conclusions Gene expression profiling for prospective response prediction was feasible in this international trial. The 30-gene predictor can identify patients with greater than average sensitivity to T/FAC chemotherapy. However, it captured molecular equivalents of clinical phenotype. Next generation predictive markers will need to be developed separately for different molecular subsets of breast cancers. PMID:20829329

mer and fac isomerism in tris chelate diimine metal complexes.

PubMed

Dabb, Serin L; Fletcher, Nicholas C

2015-03-14

In this perspective, we highlight the issue of meridional (mer) and facial (fac) orientation of asymmetrical diimines in tris-chelate transition metal complexes. Diimine ligands have long been the workhorse of coordination chemistry, and whilst there are now good strategies to isolate materials where the inherent metal centered chirality is under almost complete control, and systematic methodologies to isolate heteroleptic complexes, the conceptually simple geometrical isomerism has not been widely investigated. In systems where the two donor atoms are significantly different in terms of the σ-donor and π-accepting ability, the fac isomer is likely to be the thermodynamic product. For the diimine complexes with two trigonal planar nitrogen atoms there is much more subtlety to the system, and external factors such as the solvent, lattice packing and the various steric considerations play a delicate role in determining the observed and isolable product. In this article we discuss the possibilities to control the isomeric ratio in labile systems, consider the opportunities to separate inert complexes and discuss the observed differences in their spectroscopic properties. Finally we report on the ligand orientation in supramolecular systems where facial coordination leads to simple regular structures such as helicates and tetrahedra, but the ability of the ligand system to adopt a mer orientation enables self-assembled structures of considerable beauty and complexity.

Automated detection of pain from facial expressions: a rule-based approach using AAM

NASA Astrophysics Data System (ADS)

Chen, Zhanli; Ansari, Rashid; Wilkie, Diana J.

2012-02-01

In this paper, we examine the problem of using video analysis to assess pain, an important problem especially for critically ill, non-communicative patients, and people with dementia. We propose and evaluate an automated method to detect the presence of pain manifested in patient videos using a unique and large collection of cancer patient videos captured in patient homes. The method is based on detecting pain-related facial action units defined in the Facial Action Coding System (FACS) that is widely used for objective assessment in pain analysis. In our research, a person-specific Active Appearance Model (AAM) based on Project-Out Inverse Compositional Method is trained for each patient individually for the modeling purpose. A flexible representation of the shape model is used in a rule-based method that is better suited than the more commonly used classifier-based methods for application to the cancer patient videos in which pain-related facial actions occur infrequently and more subtly. The rule-based method relies on the feature points that provide facial action cues and is extracted from the shape vertices of AAM, which have a natural correspondence to face muscular movement. In this paper, we investigate the detection of a commonly used set of pain-related action units in both the upper and lower face. Our detection results show good agreement with the results obtained by three trained FACS coders who independently reviewed and scored the action units in the cancer patient videos.

Fac-Re(bpy)(CO){sub 3}(COOMe): A model metallocarboxylate complex of rhenium with a bipyridyl ligand

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gibson, D.H.; Sleadd, B.A.; Vij, A.

1999-05-01

The title compound fac-(2,2{prime}-bipyridyl)(carbomethoxy)rhenium tricarbonyl, crystallizes in the monoclinic system, space group P2{sub 1}/c with the following crystal data: a = 8.37551(1), b = 6.6934(1), c = 26.2098(1) {angstrom}, V({angstrom}{sup 3}) = 1,535.93(3), Z = 4, and {beta} = 90.0971(2). The metal environment is slightly distorted octahedral with a chelating bipyridyl ligand and a facial arrangement of the carbon monoxide ligands.

A Comprehensive Analysis of Multiscale Field-Aligned Currents: Characteristics, Controlling Parameters, and Relationships

NASA Astrophysics Data System (ADS)

McGranaghan, Ryan M.; Mannucci, Anthony J.; Forsyth, Colin

2017-12-01

We explore the characteristics, controlling parameters, and relationships of multiscale field-aligned currents (FACs) using a rigorous, comprehensive, and cross-platform analysis. Our unique approach combines FAC data from the Swarm satellites and the Advanced Magnetosphere and Planetary Electrodynamics Response Experiment (AMPERE) to create a database of small-scale (˜10-150 km, <1° latitudinal width), mesoscale (˜150-250 km, 1-2° latitudinal width), and large-scale (>250 km) FACs. We examine these data for the repeatable behavior of FACs across scales (i.e., the characteristics), the dependence on the interplanetary magnetic field orientation, and the degree to which each scale "departs" from nominal large-scale specification. We retrieve new information by utilizing magnetic latitude and local time dependence, correlation analyses, and quantification of the departure of smaller from larger scales. We find that (1) FACs characteristics and dependence on controlling parameters do not map between scales in a straight forward manner, (2) relationships between FAC scales exhibit local time dependence, and (3) the dayside high-latitude region is characterized by remarkably distinct FAC behavior when analyzed at different scales, and the locations of distinction correspond to "anomalous" ionosphere-thermosphere behavior. Comparing with nominal large-scale FACs, we find that differences are characterized by a horseshoe shape, maximizing across dayside local times, and that difference magnitudes increase when smaller-scale observed FACs are considered. We suggest that both new physics and increased resolution of models are required to address the multiscale complexities. We include a summary table of our findings to provide a quick reference for differences between multiscale FACs.

Directed Laplacians For Fuzzy Autocatalytic Set Of Fuzzy Graph Type-3 Of An Incineration Process

NASA Astrophysics Data System (ADS)

Ahmad, Tahir; Baharun, Sabariah; Bakar, Sumarni Abu

2010-11-01

Fuzzy Autocatalytic Set (FACS) of Fuzzy Graph Type-3 was used in the modeling of a clinical waste incineration process in Malacca. FACS provided more accurate explanations of the incineration process than using crisp graph. In this paper we explore further FACS. Directed and combinatorial Laplacian of FACS are developed and their basic properties are presented.

48 CFR 301.607-78 - Contracting Officer designation of a Program/Project Manager as the Contracting Officer's...

Code of Federal Regulations, 2013 CFR

2013-10-01

... Representative. Personnel who are FAC-P/PM certified, at any level, meet the requirements for FAC-COTR certification and are, therefore, not required to obtain FAC-COTR certification to serve as a COTR for an HHS acquisition. However, for those individuals serving as a Program or Project Manager under a FAC-P/PM...

48 CFR 301.607-78 - Contracting Officer designation of a Program/Project Manager as the Contracting Officer's...

Code of Federal Regulations, 2012 CFR

2012-10-01

... Representative. Personnel who are FAC-P/PM certified, at any level, meet the requirements for FAC-COTR certification and are, therefore, not required to obtain FAC-COTR certification to serve as a COTR for an HHS acquisition. However, for those individuals serving as a Program or Project Manager under a FAC-P/PM...

48 CFR 301.607-78 - Contracting Officer designation of a Program/Project Manager as the Contracting Officer's...

Code of Federal Regulations, 2011 CFR

2011-10-01

... Representative. Personnel who are FAC-P/PM certified, at any level, meet the requirements for FAC-COTR certification and are, therefore, not required to obtain FAC-COTR certification to serve as a COTR for an HHS acquisition. However, for those individuals serving as a Program or Project Manager under a FAC-P/PM...

48 CFR 301.607-78 - Contracting Officer designation of a Program/Project Manager as the Contracting Officer's...

Code of Federal Regulations, 2014 CFR

2014-10-01

... Representative. Personnel who are FAC-P/PM certified, at any level, meet the requirements for FAC-COTR certification and are, therefore, not required to obtain FAC-COTR certification to serve as a COTR for an HHS acquisition. However, for those individuals serving as a Program or Project Manager under a FAC-P/PM...

48 CFR 1401.603-2 - Selection.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Acquisition Certification in Contracting (FAC-C) Program Manual. Director, PAM, is the approving authority for all new and reinstated FAC-C certifications. BPCs are authorized to approve renewal FAC-C...

48 CFR 1401.603-2 - Selection.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Acquisition Certification in Contracting (FAC-C) Program Manual. Director, PAM, is the approving authority for all new and reinstated FAC-C certifications. BPCs are authorized to approve renewal FAC-C...

48 CFR 1401.603-2 - Selection.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Acquisition Certification in Contracting (FAC-C) Program Manual. Director, PAM, is the approving authority for all new and reinstated FAC-C certifications. BPCs are authorized to approve renewal FAC-C...

48 CFR 1401.603-2 - Selection.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Acquisition Certification in Contracting (FAC-C) Program Manual. Director, PAM, is the approving authority for all new and reinstated FAC-C certifications. BPCs are authorized to approve renewal FAC-C...

Tuning extreme ultraviolet emission for optimum coupling with multilayer mirrors for future lithography through control of ionic charge states

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ohashi, Hayato, E-mail: ohashi@cc.utsunomiya-u.ac.jp; Higashiguchi, Takeshi, E-mail: higashi@cc.utsunomiya-u.ac.jp; Suzuki, Yuhei

2014-01-21

We report on the identification of the optimum plasma conditions for a laser-produced plasma source for efficient coupling with multilayer mirrors at 6.x nm for beyond extreme ultraviolet lithography. A small shift to lower energies of the peak emission for Nd:YAG laser-produced gadolinium plasmas was observed with increasing laser power density. Charge-defined emission spectra were observed in electron beam ion trap (EBIT) studies and the charge states responsible identified by use of the flexible atomic code (FAC). The EBIT spectra displayed a larger systematic shift of the peak wavelength of intense emission at 6.x nm to longer wavelengths with increasingmore » ionic charge. This combination of spectra enabled the key ion stage to be confirmed as Gd{sup 18+}, over a range of laser power densities, with contributions from Gd{sup 17+} and Gd{sup 19+} responsible for the slight shift to longer wavelengths in the laser-plasma spectra. The FAC calculation also identified the origin of observed out-of-band emission and the charge states responsible.« less

MEASUREMENT AND MODELING OF Na-LIKE Fe XVI INNER-SHELL SATELLITES BETWEEN 14.5 A AND 18 A

DOE Office of Scientific and Technical Information (OSTI.GOV)

Graf, A.; Beiersdorfer, P.; Brown, G. V.

2009-04-20

We have used the University of California Lawrence Livermore National Laboratory's EBIT-I electron beam ion trap to perform measurements of the wavelengths and relative intensities of the X-ray lines from inner-shell satellite transitions in sodium-like Fe XVI. The measurements were carried out with high-resolution crystal and grating spectrometers and covered the 14.5-18 A wavelength band. In contrast to some predicted line strengths and positions found in the literature, our results show that the strongest relatively unblended inner-shell satellites of Fe XVI are located near 15.2 A. This is near the location of the 3d{yields} 2p intercombination line in Fe XVII.more » Calculations using the Flexible Atomic Code (FAC) are presented. The average deviation between the EBIT-I measurements and the FAC calculations for the wavelength positions and line ratios are 22 mA and a factor of 2.3, respectively, where the average is taken over the ten features included in this work.« less

Study of field-aligned current (FAC), interplanetary electric field component (Ey), interplanetary magnetic field component (Bz), and northward (x) and eastward (y) components of geomagnetic field during supersubstorm

NASA Astrophysics Data System (ADS)

Adhikari, Binod; Dahal, Subodh; Chapagain, Narayan P.

2017-05-01

A dominant process by which energy and momentum are transported from the magnetosphere to the ionosphere is known as field-aligned current (FAC). It is enhanced during magnetic reconnection and explosive energy release at a substorm. In this paper, we studied FAC, interplanetary electric field component (Ey), interplanetary magnetic field component (Bz), and northward (x) and eastward (y) components of geomagnetic field during three events of supersubstorm occurred on 24 November 2001, 21 January 2005, and 24 August 2005. Large-scale FAC, supposed to be produced during supersubstorm (SSS), has potentiality to cause blackout on Earth. We examined temporal variations of the x and y components of high-latitude geomagnetic field during SSS, which is attributed to the FACs. We shall report the characteristics of high-latitude northward and eastward components of geomagnetic field variation during the growth phase of SSS by the implementation of discrete wavelet transform (DWT) and cross-correlation analysis. Among three examples of SSS events, the highest peak value of FAC was estimated to be 19 μAm-2. This is shore up with the prediction made by Parks (1991) and Stasiewicz et al. (1998) that the FACs may vary from a few tens to several hundred μAm-2. Although this peak value of FACs for SSS event is much higher than the average FACs associated with regular substorms or magnetic storms, it is expedient and can be expect for SSS events which might be due to very high density solar wind plasma parcels (PPs) triggering the SSS events. In all events, during growth phase, the FAC increases to extremely high level and the geomagnetic northward component decreases to extremely low level. This represents a strong positive correlation between FAC and geomagnetic northward component. The DWT analysis accounts that the highest amplitude of the wavelet coefficients indicates singularities present in FAC during SSS event. But the amplitude of squared wavelet coefficient is found to be different from each other, which might be due to the solar wind PPs of different density triggering the SSS events. The cross-correlation analysis suggests that the perturbation on geomagnetic northward component at high latitude during SSS strongly correlates with the fluctuation pattern of FAC density. Hence, the FAC is the primary sources for the eastward-westward magnetic field perturbations at high latitude.

48 CFR 301.607-71 - FAC-P/PM levels and requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... GENERAL HHS ACQUISITION REGULATION SYSTEM Career Development, Contracting Authority, and Responsibilities..., systems engineering, test and evaluation, contracting, and business. (6) Specific core competencies also...

fac-[Re(CO)3(dmso-O)3](CF3SO3): a new versatile and efficient Re(I) precursor for the preparation of mono and polynuclear compounds containing fac-[Re(CO)3]+ fragments.

PubMed

Casanova, Massimo; Zangrando, Ennio; Munini, Fabio; Iengo, Elisabetta; Alessio, Enzo

2006-11-14

We show here that the new complex fac-[Re(CO)3(dmso-O)3](CF3SO3) (1), efficiently prepared in one step from [ReBr(CO)5] and featuring a broad range of solubility, is, in general, a better precursor for the one-step synthesis of mono- and polynuclear inorganic compounds containing fac-[Re(CO)3]+ fragments compared to the commonly used (NEt4)2fac-[ReBr3(CO)3] and fac-[Re(CO)3(CH3CN)3](Y) (Y = PF6, BF4, ClO4) species. Compound 1 is the first example of a Re(I)-dmso complex structurally characterized and confirms the rule that dmso is always O-bonded when trans to CO. The reactivity of 1 was tested in the one-step preparation of several new and known complexes. The O-bonded sulfoxides of 1 are replaced under mild conditions by tri- (L3) and bidentate ligands (L2) to produce fac-[Re(CO)3(L3)]+ and fac-[Re(CO)3(L2)(dmso-O)]+ compounds, respectively. An excess of monodentate ligands (L) and more forcing conditions are needed to prepare fac-[Re(CO)3(L)3]+ compounds. The new compounds include fac-[Re(CO)3(bipy)(dmso-O)](CF3SO3) (4), that turned out to be an excellent precursor for binding the luminescent fac-[Re(CO)3(bipy)]+ fragment to polytopic ligands for the construction of more elaborate assemblies. One example reported here is the two-step preparation of fac-[{Re(CO)3(bipy)}(mu-4,4'-bipy){Ru(TPP)(CO)}](CF3SO3) (8) (TPP = tetraphenylporphyrin). The X-ray structures of the new compounds 1, 4, of the bis-porphyrin complex fac-[Re(CO)3Cl(4'MPyP)2] (13) (4'MPyP = 5-(4'pyridyl)-10,15,20-triphenylporphyrin), and of the rhenium-cyclophane [{(CO)3Re(mu-OH)2Re(CO)3}2(micro-4,4'-bipy)2] (15), among others, are described. Compound 1 might find useful applications in supramolecular chemistry (metal-mediated assembly of large architectures), in the in situ preparation of stable Re compounds to be used in nuclear medicine, and for the labeling of biomolecules.

D-Serine rescues the deficits of hippocampal long-term potentiation and learning and memory induced by sodium fluoroacetate.

PubMed

Han, Huili; Peng, Yan; Dong, Zhifang

2015-06-01

It is well known that bidirectional glia-neuron interactions play important roles in the neurophysiological and neuropathological processes. It is reported that impairing glial functions with sodium fluoroacetate (FAC) impaired hippocampal long-term depression (LTD) and spatial memory retrieval. However, it remains unknown whether FAC impairs hippocampal long-term potentiation (LTP) and learning and/or memory, and if so, whether pharmacological treatment with exogenous d-serine can recuse the impairment. Here, we reported that systemic administration of FAC (3mg/kg, i.p.) before training resulted in dramatic impairments of spatial learning and memory in water maze and fear memory in contextual fear conditioning. Furthermore, the behavioral deficits were accompanied by impaired LTP induction in the hippocampal CA1 area of brain slices. More importantly, exogenous d-serine treatment succeeded in recusing the deficits of hippocampal LTP and learning and memory induced by FAC. Together, these results suggest that astrocytic d-serine may be essential for hippocampal synaptic plasticity and memory, and that alteration of its levels may be relevant to the induction and potentially treatment of psychiatric and neurological disorders. Copyright © 2015 Elsevier Inc. All rights reserved.

Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice

PubMed Central

Shinya, Tomonori; Hojo, Yuko; Desaki, Yoshitake; Christeller, John T.; Okada, Kazunori; Shibuya, Naoto; Galis, Ivan

2016-01-01

Induced plant defense responses against insect herbivores are triggered by wounding and/or perception of herbivore elicitors from their oral secretions (OS) and/or saliva. In this study, we analyzed OS isolated from two rice chewing herbivores, Mythimna loreyi and Parnara guttata. Both types of crude OS had substantial elicitor activity in rice cell system that allowed rapid detection of early and late defense responses, i.e. accumulation of reactive oxygen species (ROS) and defense secondary metabolites, respectively. While the OS from M. loreyi contained large amounts of previously reported insect elicitors, fatty acid-amino acid conjugates (FACs), the elicitor-active P. guttata’s OS contained no detectable FACs. Subsequently, elicitor activity associated with the high molecular mass fraction in OS of both herbivores was identified, and shown to promote ROS and metabolite accumulations in rice cells. Notably, the application of N-linolenoyl-Gln (FAC) alone had only negligible elicitor activity in rice cells; however, the activity of isolated elicitor fraction was substantially promoted by this FAC. Our results reveal that plants integrate various independent signals associated with their insect attackers to modulate their defense responses and reach maximal fitness in nature. PMID:27581373

Structure and Properties of fac-[Re(I)(CO)3(NTA)](2-) (NTA(3-) = Trianion of Nitrilotriacetic Acid) and fac-[Re(I)(CO)3(L)](n-) Analogues Useful for Assessing the Excellent Renal Clearance of the fac-[(99m)Tc(I)(CO)3(NTA)](2-) Diagnostic Renal Agent.

PubMed

Klenc, Jeffrey; Lipowska, Malgorzata; Abhayawardhana, Pramuditha L; Taylor, Andrew T; Marzilli, Luigi G

2015-07-06

We previously identified two new agents based on the [(99m)Tc(V)O](3+) core with renal clearances in human volunteers 30% higher than that of the widely used clinical tracer (99m)Tc-MAG3 (MAG3(5-) = penta-anion of mercaptoacetyltriglycine). However, renal agents with even higher clearances are needed. More recently, we changed our focus from the [(99m)Tc(V)O](3+) core to the discovery of superior tracers based on the fac-[(99m)Tc(I)(CO)3](+) core. Compared to (99m)Tc-MAG3, fac-[(99m)Tc(I)(CO)3(NTA)](2-) (NTA(3-) = trianion of nitrilotriacetic acid) holds great promise by virtue of its efficient renal clearance via tubular secretion and the absence of hepatobiliary elimination, even in patients with severely reduced renal function. We report here NMR, molecular (X-ray) structure, and solution data on fac-[Re(I)(CO)3(NTA)](2-) with a -CH2CO2(-) dangling monoanionic chain and on two fac-[Re(I)(CO)3(L)](-) analogues with either a -CH2CONH2 or a -CH2CH2OH dangling neutral chain. In these three fac-[Re(I)(CO)3(L)](n-) complexes, the fac-[Re(I)(CO)3(N(CH2CO2)2)](-) moiety is structurally similar and has similar electronic properties (as assessed by NMR data). In reported and ongoing studies, the two fac-[(99m)Tc(I)(CO)3(L)](-) analogues with these neutral dangling chains were found to have pharmacokinetic properties very similar to those of fac-[(99m)Tc(I)(CO)3(NTA)](2-). Therefore, we reach the unexpected conclusion that in fac-[(99m)Tc(I)(CO)3(L)](n-) agents, renal clearance is affected much more than anticipated by features of the core plus the chelate rings (the [(99m)Tc(I)(CO)3(N(CH2CO2)2)](-) moiety) than by the presence of a negatively charged dangling carboxylate chain.

Antinociceptive and anti-edema properties of the ethyl acetate fraction obtained from extracts of Coriandrum sativum Linn. leaves.

PubMed

Begnami, Andreza Fabiana; Spindola, Humberto M; Ruiz, Ana Lucia T Gois; de Carvalho, João Ernesto; Groppo, Francisco Carlos; Rehder, Vera L Garcia

2018-07-01

This study evaluated the antinociceptive and anti-edema properties of fractions of Coriandrum sativum Linn. (Apiaceae/Umbelliferae) leaves in mice. Ethyl acetate fractions (FAc) were obtained from dichloromethane extracts prepared from dried C. sativum (CS) leaves and stems. The effects of different concentrations of FAc on mice were observed using the open-field test, formalin-, capsaicin-, and carrageenan-induced paw edema tests, and the acetic acid-induced abdominal writhing test. Results from the carrageenan-induced paw edema test were subjected to a linear regression analysis and data from other assays were subjected to the Kruskal-Wallis test (followed by the SNK post hoc test). Dihydrocoriandrin (34.5%), coriandrin (14.4%), vitamin E (4.6%), and stigmasterol (7.9%) were identified in FAc. The number of squares the mice crossed in the open field test was decreased by 100 mg/kg and 300 mg/kg FAc (i.p.). The administration of 30, 100, and 300 mg/kg FAc induced fewer abdominal writhes than the control. In the formalin test, neurogenic pain was reduced by 20 mg/kg morphine and 30 and 100 mg/kg FAc, but not 5 mg/kg dexamethasone or 10 mg/kg FAc. Formalin-induced inflammatory pain was decreased by morphine, dexamethasone, and 30 and 100 mg/kg FAc. Morphine and 30, 100, and 300 mg/kg FAc significantly decreased the reaction time during the capsaicin test. Dexamethasone reduced both early and later phases of carrageenan-induced edema. Both 30 and 300 mg/kg FAc induced less edema than the control throughout the experiment. FAc showed antinociceptive, anti-edema and anti-inflammatory properties and it may be considered as a potential phytotherapeutic agent in the future. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

In Vitro and In Vivo Comparison of Gemcitabine and the Gemcitabine Analog 1-(2'-deoxy-2'-fluoroarabinofuranosyl) Cytosine (FAC) in Human Orthotopic and Genetically Modified Mouse Pancreatic Cancer Models.

PubMed

Russell, James; Pillarsetty, Nagavarakishore; Kramer, Robin M; Romesser, Paul B; Desai, Pooja; Haimovitz-Friedman, Adriana; Lowery, Maeve A; Humm, John L

2017-12-01

Although gemcitabine is a mainstay of pancreatic cancer therapy, it is only moderately effective, and it would be desirable to measure drug uptake in patients. 1-(2'-deoxy-2'-fluoroarabinofuranosyl) cytosine (FAC), is an analog of gemcitabine, and when labeled with F-18, it may be a potential surrogate PET tracer for the drug. [ 18 F]FAC was synthesized to a radiochemical purity of >96 %. The human tumor lines AsPC1, BxPC3, Capan-1, Panc1, and MiaPaca2 were grown orthotopically in nude mice. KPC mice that conditionally express oncogenic K-ras and p53 mutations in pancreatic tissue were also used. The intra-tumoral distributions of [ 14 C]gemcitabine and [ 18 F]FAC were mapped with autoradiography. The inter-tumor correlation between [ 14 C]gemcitabine and [ 18 F]FAC was established in the orthotopic tumors. Expression of the equilibrative and concentrative nucleoside transporters (ENT, CNT) in vitro was detected by western blotting. Drug uptake was characterized in vitro using [ 3 H]gemcitabine and the effect of transporter inhibition on gemcitabine and FAC uptake was investigated. The relative affinity of cells for gemcitabine and FAC was tested in competition assays. The cell lines differed in sensitivity to transport inhibitors and in competition studies. There was a good in vivo correlation between the total uptake of [ 18 F]FAC and [ 14 C]gemcitabine, measured across all orthotopic tumors. Using the KPC and BxPC3 models, we found that [ 14 C]gemcitabine and [ 18 F]FAC were largely co-localized. In the lines examined here, [ 18 F]FAC uptake correlates well with gemcitabine in vivo, supporting the notion that [ 18 F]FAC can serve as a PET radiotracer surrogate to determine the uptake and distribution of gemcitabine within pancreatic tumors.

In Vitro and In Vivo Comparison of Gemcitabine and the Gemcitabine Analog 1-(2′-deoxy-2′-fluoroarabinofuranosyl) Cytosine (FAC) in Human Orthotopic and Genetically Modified Mouse Pancreatic Cancer Models

PubMed Central

Russell, James; Pillarsetty, Nagavarakishore; Kramer, Robin M; Romesser, Paul B; Desai, Pooja; Haimovitz-Friedman, Adriana; Lowery, Maeve A; Humm, John L

2017-01-01

Purpose Although gemcitabine is a mainstay of pancreatic cancer therapy, it is only moderately effective, and it would be desirable to measure drug uptake in patients. 1-(2′-deoxy-2′-fluoroarabinofuranosyl) cytosine (FAC), is an analog of gemcitabine, and when labeled with F-18, it may be a potential surrogate PET tracer for the drug. Procedures [18F]FAC was synthesized to a radiochemical purity of >96 %. The human tumor lines AsPC1, BxPC3, Capan-1, Panc1, and MiaPaca2 were grown orthotopically in nude mice. KPC mice that conditionally express oncogenic K-ras and p53 mutations in pancreatic tissue were also used. The intra-tumoral distributions of [14C]gemcitabine and [18F]FAC were mapped with autoradiography. The inter-tumor correlation between [14C]gemcitabine and [18F]FAC was established in the orthotopic tumors. Expression of the equilibrative and concentrative nucleoside transporters (ENT, CNT) in vitro was detected by western blotting. Drug uptake was characterized in vitro using [3H]gemcitabine and the effect of transporter inhibition on gemcitabine and FAC uptake was investigated. The relative affinity of cells for gemcitabine and FAC was tested in competition assays. The cell lines differed in sensitivity to transport inhibitors and in competition studies. There was a good in vivo correlation between the total uptake of [18F]FAC and [14C]gemcitabine, measured across all orthotopic tumors. Using the KPC and BxPC3 models, we found that [14C]gemcitabine and [18F]FAC were largely co-localized. Conclusions In the lines examined here, [18F]FAC uptake correlates well with gemcitabine in vivo, supporting the notion that [18F]FAC can serve as a PET radiotracer surrogate to determine the uptake and distribution of gemcitabine within pancreatic tumors. PMID:28349292

Flow-accelerated corrosion in power plants. Revision 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chexal, B.; Horowitz, J.; Dooley, B.

1998-07-01

Flow-Accelerated Corrosion (FAC) is a phenomenon that results in metal loss from piping, vessels, and equipment made of carbon steel. FAC occurs only under certain conditions of flow, chemistry, geometry, and material. Unfortunately, those conditions are common in much of the high-energy piping in nuclear and fossil-fueled power plants. Undetected, FAC will cause leaks and ruptures. Consequently, FAC has become a major issue, particularly for nuclear plants. Although major failures are rare, the consequences can be severe. In 1986, four men in the area of an FAC-induced pipe rupture were killed. Fossil plants too, are subject to FAC. In 1995,more » a failure at a fossil-fired plant caused two fatalities. In addition to concerns about personnel safety, FAC failures can pose challenges to plant safety. Regulatory agencies have therefore required nuclear utilities to institute formal programs to address FAC. Finally, a major FAC failure (like the one that happened in 1997 at a US nuclear power plant) can force a plant to shutdown and purchase replacement power at a price approaching a million dollars per day depending upon the MWe rating of the plant. A great deal of time and money has been spent to develop the technology to predict, detect, and mitigate FAC in order to prevent catastrophic failures. Over time, substantial progress has been made towards understanding and preventing FAC. The results of these efforts include dozens of papers, reports, calculations, and manuals, as well as computer programs and other tools. This book is written to provide a detailed treatment of the entire subject in a single document. Any complex issue requires balancing know-how, the risk of decision making, and a pragmatic engineering solution. This book addresses these by carrying out the necessary R and D and engineering along with plant knowledge to cover all quadrants of Chexal`s four quadrant known-unknown diagram, as seen in Figure i.« less

Free Active Chlorine in Sodium Hypochlorite Solutions Admixed with Octenidine, SmearOFF, Chlorhexidine, and EDTA.

PubMed

Krishnan, Unni; Saji, Sreeja; Clarkson, Roger; Lalloo, Ratilal; Moule, Alex J

2017-08-01

The therapeutic effects of sodium hypochlorite (NaOCl) solutions are dependent on the levels of free available chlorine (FAC). Mixing these solutions with irrigants can result in significant reductions in FAC. Although the effect of some irrigants on FAC is known, the effect of other commonly used irrigants is not. Thus, the therapeutic ramifications of the concurrent use of these on the efficiency of NaOCl solutions is not known. Aliquots of 5.2% (w/v) NaOCl solutions were admixed in proportions of 90:10, 80:20, and 50:50 with the following irrigants: octenidine dihydrochloride (OCT); SmearOFF (Vista Dental Products, Racine, WI), 17% EDTA; and 0.2%, 2%, and 5% chlorhexidine (CHX) solutions. Changes in FAC were measured by iodometric titration. Statistical differences between means were determined using a post hoc Tukey analysis test after an analysis of variance. OCT appeared not to affect FAC and was significantly different than all other irrigants, except for 90:10 and 80:20 mixtures of low concentration (0.2%) CHX. CHX solutions showed a marked concentration- and mixture proportion-dependent detrimental effect on FAC. The reduction of FAC between different concentrations of CHX was statistically significant in 80:20 and 50:50 proportions, with 50:50 mixtures of 5% CHX having the greatest influence. Mixtures containing even small proportions of SmearOFF or EDTA exhibited significant losses in FAC. OCT has little effect on FAC and can be used concurrently with NaOCl solutions. Higher concentrations of CHX significantly affect FAC. Their combined use with NaOCl solutions should be avoided. EDTA and SmearOFF should not be mixed with NaOCl solutions. Copyright © 2017 American Association of Endodontists. All rights reserved.

Substorm Birkeland currents and Cowling channels in the ionosphere

NASA Astrophysics Data System (ADS)

Fujii, R.

2016-12-01

Field-aligned current (FAC) connects electromagnetically the ionosphere with the magnetosphere and plays important roles on dynamics and energetics in the magnetosphere and the ionosphere. In particular, connections between FACs in the ionosphere give important information on various current sources in the magnetosphere and the linkage between them, although the connection between FACs in the ionosphere does not straightforwardly give that in the magnetosphere. FACs in the ionosphere are closed to each other through ionospheric currents determined with the electric field and the Hall and Pedersen conductivities. The electric field and the conductivities are not independently distributed, but rather they are harmonized with each other spatially and temporarily in a physically consistent manner to give a certain FAC. In particular, the divergence of the Hall current due to the inhomogeneity of the Hall conductivity either flows in/out to the magnetosphere as a secondary FAC or accumulates excess charges that produce a secondary electric field. This electric field drives a current circuit connecting the Hall current with the Pedersen current; a Cowling channel current circuit. The FAC (the electric field) we observe is the sum of the primary and secondary FACs (electric fields). The talk will present characteristics of FACs and associated electric field and auroras during substorms, and the ionospheric current closures between the FACs. A statistical study has shown that the majority of region 1 currents are connected to their adjacent region 2 or region 0 currents, indicating the Pedersen current closure rather than the Hall current closure is dominant. On the other hand, the Pedersen currents associated with surge and substorm-related auroras often are connected to the Hall currents, forming a Cowling channel current circuit within the ionosphere.

Fluorescent aromatic hydrocarbons in bile as a biomarker of exposure of brown bullheads (Ameiurus nebulosus) to contaminated sediments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Leadly, T.A.; Haffner, G.D.; Arcand-Hoy, L.D.

1999-04-01

Analysis of fluorescent aromatic compounds (FACs) in the bile of fish has been widely used as a biomarker of exposure to polynuclear aromatic hydrocarbon (PAH) contamination. However, bile FAC data for feral fish populations are typically highly variable, and in a few cases, elevation of FACs has not been observed in fish from contaminated areas. In this study, the bile FACs and hepatic ethoxyresorufin-O-deethylase activity in brown bullheads (Ameiurus nebulosus) exposed in the laboratory to contaminated sediments from Hamilton Harbour, Ontario, Canada, increased by 173-fold within 72 h of initial exposure and rapidly declined thereafter. In bullheads caged in themore » contaminated Trenton Channel area of the Detroit River, bile FACs also increased rapidly within 4 d of initial exposure to mean levels >3,000 ng of benzo[a]pyrene equivalents per milliliter of bile. Surprisingly, there was no difference in the mean-levels of bile FACs in fish caged above the sediment versus fish caged in direct contact with the sediment, indicating that water may be the major vector for uptake of PAHs. The lower bile FACs in bullheads caged in other regions of the Detroit River were consistent with the lower concentrations of PAHs in the sediments from these areas. These data indicate that bile FAC levels are a biomarker of recent exposure to aromatic hydrocarbons in sediments. However, FAC data were highly variable even in these studies, in which fish were relatively homogenous in size, feeding status, and exposure history. Therefore, Even higher variability in bile FAC data are expected in biomarker studies as a result of differences in reproductive status, size, diet, and mobility of the fish.« less

The interplanetary magnetic field B sub y -dependent field-aligned current in the dayside polar cap under quiet conditions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yamauchi, M.; Araki, T.

1989-03-01

Spatial distribution and temporal variation of the interplanetary magnetic field (IMF) B{sub y}-dependent cusp region field-aligned currents (FACs) during quiet periods were studied by use of magnetic data observed by Magsat. The analysis was made for 11 events (each event lasts more than one and a half days) when the IMF B{sub y} component was steadily large and B{sub x} was relatively small ({vert bar}B{sub z}{vert bar} < {vert bar}B{sub y}{vert bar}). Results of the analysis of total 62 half-day periods for the IMF B{sub y}-dependent cusp region FAC are summarized as follows: (1) the IMF B{sub y}-dependent cusp regionmore » FAC is located at around 86{degree}-87{degree} invariant latitude local noon, which is more poleward than the location of the IMF B{sub z}-dependent cusp region FAC; (2) the current density of this FAC is greater than previous studies ({ge} 4 {mu}A/m{sup 2} for IMF B{sub y} = 6 nT); (3) there are two time scales for the IMF B{sub y}-dependent cusp region FAC to appear: the initial rise of the current is on a short time scale, {approximately} 10 min, and it is followed by a gradual increase on a time scale of several hours to a half day; (4) the seasonal change of this FAC is greater than that of the nightside region 1 or region 2 FACs; (5) the IMF B{sub z}-dependent cusp region FAC is not well observed around the cusp when the IMF B{sub y}-dependent cusp region FAC is intense.« less

Field-Aligned Current at Plasma Sheet Boundary Layers During Storm Time: Cluster Observation

NASA Astrophysics Data System (ADS)

Shi, J.; Cheng, Z.; Zhang, T.; Dunlop, M.; Liu, Z.

2007-05-01

The magnetic field data from the FGM instruments on board the four Cluster spacecrafts were used to study Field Aligned Current (FAC) at the Plasma Sheet Boundary Layers (PSBLs) with the so called "curlometer technique". We analyzed the date obtained in 2001 in the magnetotail and only two cases were found in the storm time. One (August 17, 2001) occurred from sudden commencement to main phase, and the other (October 1, 2001) lay in the main phase and recovery phase. The relationship between the FAC density and the AE index was studied and the results are shown as follows. (1) In the sudden commencement and the main phase the density of the FAC increases obviously, in the recovery phase the density of the FAC increases slightly. (2) From the sudden commencement to the initial stage of the main phase the FAC increases with decreasing AE index and decreases with increasing AE index. From the late stage of the main phase to initial stage of the recovery phase, the FAC increases with increasing AE index and decreases with decreasing AE index. In the late stage of the recovery phase the disturbance of the FAC is not so violent, so that the FAC varying with the AE index is not very obvious.

The influence of IMF cone angle on invariant latitudes of polar region footprints of FACs in the magnetotail: Cluster observatio

NASA Astrophysics Data System (ADS)

Cheng, Z.; Shi, J.; Zhang, J.; Kistler, L. M.

2017-12-01

The influence of the interplanetary magnetic field (IMF) cone angle θ (the angle between the IMF direction and the Sun-Earth line) on the invariant latitudes (ILATs) of the footprints of the field-aligned currents (FACs) in the magnetotail has been investigated. We performed a statistic study of 542 FAC cases observed by the four Cluster spacecraft in the northern hemisphere. The results show that the large FAC (>10 nA/m2) cases occur at the low ILATs (<71 º) and mainly occur when the IMF cone angle θ>60º, which implies the footprints of the large FACs mainly expand equatorward with large IMF cone angle. The equatorward boundary of the FAC footprints in the polar region decreases with the IMF cone angle especially when IMF Bz is positive. There is almost no correlation or a weak positive correlation of the poleward boundary and IMF cone angle no matter IMF is northward or southward. The equatorward boundary is more responsive to the IMF cone angle. Compared to the equatorward boundary, the center of the FAC projected location changes very little. This is the first time a correlation between FAC projected location and IMF cone angle has been determined.

Utilization of operating experience to prevent piping failures at steam plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adams, T.S.; Dietrich, E.B.

1999-11-01

The key to preventing flow-accelerated corrosion (FAC) induced piping failures in steam plants is the development and implementation of a methodical program for assessing plant susceptibility to FAC and managing the effects of FAC. One of the key elements of an effective FAC program is the accurate and comprehensive utilization of plant-specific and industry-wide operating experience. Operating experience should be used to develop the program to identify specific areas for inspection or replacement, and to maintain an effective program. This paper discusses the utilization of operating experience in FAC programs at nuclear power plants, fossil plants and other steam plants.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anderson, Danielle; Siegbahn, Albert; Fallone, Gin

Purpose: The BioMedical Imaging and Therapy (BMIT) beamlines at the Canadian Light Source offer the opportunity for investigating novel imaging and therapy applications of synchrotron radiation. A necessary component in advancing this research, and in progressing toward clinical applications, is the availability of accurate dosimetry that is traceable to a standards institution. However, dosimetry in this setting is challenging. These beams are typically small, non-uniform, and highly intense. This work describes air kerma rate measurements on a BMIT beamline using a free-air ionization chamber (FAC). Methods: The measurements were taken at the 05B1-1 beamline (∼8 – 100 keV) for severalmore » beam qualities with mean energies between 20.0 and 84.0 keV. The Victoreen Model 480 cylindrical FAC, with a specially fabricated 0.52 mm diameter aperture, was used to measure air kerma rates. The required correction factors were determined using a variety of methods: tabulated data, measurements, theoretical calculations and Monte Carlo simulations (EGSnrc user code egs-fac). Results: The experimental air kerma rates measured between 0.270 ± 13.6% and 312 ± 2.7% Gy/min. At lower energies (low filtration), the most impactful correction factors were those for ion recombination and for x-ray attenuation. Conclusions: These measurements marked the first absolute dosimetry performed at the BMIT beamlines. The experimental and Monte Carlo methods developed will allow air kerma rates to be measured under other experimental conditions, provide a benchmark to which other dosimeters will be compared, and provide a reference for imaging and therapy research programs on this beamline.« less

Modeled ground magnetic signatures of flux transfer events

NASA Technical Reports Server (NTRS)

Mchenry, Mark A.; Clauer, C. Robert

1987-01-01

The magnetic field on the ground due to a small (not greater than 200 km scale size) localized field-aligned current (FAC) system interacting with the ionosphere is calculated in terms of an integral over the ionospheric distribution of FAC. Two different candidate current systems for flux transfer events (FTEs) are considered: (1) a system which has current flowing down the center of a cylindrical flux tube with a return current uniformly distributed along the outside edge; and (2) a system which has upward current on one half of the perimeter of a cylindrical flux tube with downward current on the opposite half. The peak magnetic field on the ground is found to differ by a factor of 2 between the two systems, and the magnetic perturbations are in different directions depending on the observer's position.

Ligand functionalization as a deactivation pathway in a fac-Ir(ppy)3-mediated radical addition.

PubMed

Devery Iii, James J; Douglas, James J; Nguyen, John D; Cole, Kevin P; Flowers Ii, Robert A; Stephenson, Corey R J

2015-01-01

Knowledge of the kinetic behavior of catalysts under synthetically relevant conditions is vital for the efficient use of compounds that mediate important transformations regardless of their composition or driving force. In particular, these data are of great importance to add perspective to the growing number of applications of photoactive transition metal complexes. Here we present kinetic, synthetic, and spectroscopic evidence of the mechanistic behavior of fac -Ir(ppy) 3 in a visible light-mediated radical addition to 3-methylindole, demonstrating the instability of fac -Ir(ppy) 3 under these conditions. During the reaction, rapid in situ functionalization of the photocatalyst occurs, eventually leading to deactivation. These findings demonstrate a conceivable deactivation process for catalytic single electron reactions in the presence of radicophilic ligands. Attempts to inhibit photocatalyst deactivation through structural modification provide further insight into catalyst selection for a given system of interest.

Historical Techniques of Lie Detection

PubMed Central

Vicianova, Martina

2015-01-01

Since time immemorial, lying has been a part of everyday life. For this reason, it has become a subject of interest in several disciplines, including psychology. The purpose of this article is to provide a general overview of the literature and thinking to date about the evolution of lie detection techniques. The first part explores ancient methods recorded circa 1000 B.C. (e.g., God’s judgment in Europe). The second part describes technical methods based on sciences such as phrenology, polygraph and graphology. This is followed by an outline of more modern-day approaches such as FACS (Facial Action Coding System), functional MRI, and Brain Fingerprinting. Finally, after the familiarization with the historical development of techniques for lie detection, we discuss the scope for new initiatives not only in the area of designing new methods, but also for the research into lie detection itself, such as its motives and regulatory issues related to deception. PMID:27247675

Long Range Transport of War-Related Burn Casualties

DTIC Science & Technology

2008-02-01

Long Range Transport of War-Related Burn Casualties Evan M. Renz, MD, FACS, Leopoldo C . Cancio, MD, FACS, David J. Barillo, MD, FACS, Christopher E...White, MD, FACS, Michael C . Albrecht, MD, Charles K. Thompson, PA- C , Jody L. Ennis, RN, BSN, Sandra M. Wanek, MD, James A. King, MD, FACEP, Kevin K...sched- Submitted for publication October 29, 2007. Accepted for publication October 30, 2007. Copyright © 2008 by Lippincott Williams & Wilkins From the

Intermolecular interactions and aggregation of fac-tris(2-phenylpyridinato-C2,N)iridium(III) in nonpolar solvents.

PubMed

Takayasu, Satoshi; Suzuki, Takayoshi; Shinozaki, Kazuteru

2013-08-15

The intermolecular interaction and aggregation of the neutral complex fac-tris(2-phenylpyridinato-C(2),N)iridium(III) (fac-Ir(ppy)3) in solution was investigated. Intermolecular interactions were found to effectively decrease the luminescence lifetime via self-quenching with increasing fac-Ir(ppy)3 concentrations. A Stern-Volmer plot for quenching in acetonitrile was linear, due to bimolecular self-quenching, but curved in toluene as the result of excimer formation. (1)H NMR spectra demonstrated a monomer-aggregate equilibrium which resulted in spectral shifts depending on solvent polarity. X-ray crystallography provided structural information concerning the aggregate, which is based on a tetramer consisting of two Δ-fac-Ir(ppy)3-Λ-fac-Ir(ppy)3 pairs. Offset π-π stacking of ppy ligands and electrostatic dipole-dipole interactions between complex molecules play an important role in the formation of these molecular pairs.

Field-aligned currents' scale analysis performed with the Swarm constellation

NASA Astrophysics Data System (ADS)

Lühr, Hermann; Park, Jaeheung; Gjerloev, Jesper W.; Rauberg, Jan; Michaelis, Ingo; Merayo, Jose M. G.; Brauer, Peter

2015-01-01

We present a statistical study of the temporal- and spatial-scale characteristics of different field-aligned current (FAC) types derived with the Swarm satellite formation. We divide FACs into two classes: small-scale, up to some 10 km, which are carried predominantly by kinetic Alfvén waves, and large-scale FACs with sizes of more than 150 km. For determining temporal variability we consider measurements at the same point, the orbital crossovers near the poles, but at different times. From correlation analysis we obtain a persistent period of small-scale FACs of order 10 s, while large-scale FACs can be regarded stationary for more than 60 s. For the first time we investigate the longitudinal scales. Large-scale FACs are different on dayside and nightside. On the nightside the longitudinal extension is on average 4 times the latitudinal width, while on the dayside, particularly in the cusp region, latitudinal and longitudinal scales are comparable.

Army Air Force Exchange Service Service (AAFES) Station Tyndall Air Force Base, Florida Final Tiered Environmental Assessment

DTIC Science & Technology

2010-04-20

Unts orl!i_gh t operations : t ~cr£_fore._l]~_i mpact~ to airspau: usc and management. Noise Increased noi se from construct ion and de mol it ion...DEP Northwest District Branch Office in Panama City confirms that a storm water managemen t system and storm water environmental resource permit...il ities and to construc t one bu ildi ng (7,320 square feet) to replace these old and inadequate fac iliti es. The new fac ili ty would include a

Mesoscale Magnetosphere-Ionosphere Coupling along Open Magnetic Field Lines Associated with Airglow Patches: Field-aligned Currents and Precipitation

NASA Astrophysics Data System (ADS)

Zou, Y.; Nishimura, Y.; Lyons, L. R.; Shiokawa, K.; Burchill, J. K.; Knudsen, D. J.; Buchert, S. C.; Chen, S.; Nicolls, M. J.; Ruohoniemi, J. M.; McWilliams, K. A.; Nishitani, N.

2016-12-01

Although airglow patches are traditionally regarded as high-density plasma unrelated to local field-aligned currents (FACs) and precipitation, past observations were limited to storm-time conditions. Recent non-storm time observations show patches to be associated with azimuthally narrow ionospheric fast flow channels that substantially contribute to plasma transportation across the polar cap and connect dayside and nightside explosive disturbances. We examine whether non-storm time patches are related also to localized polar cap FACs and precipitation using Swarm- and FAST-imager-radar conjunctions. In Swarm data, we commonly (66%) identify substantial magnetic perturbations indicating FAC enhancements around patches. These FACs have substantial densities (0.1-0.2 μA/m-2) and can be approximated as infinite current sheets (typically 75 km wide) orientated roughly parallel to patches. They usually exhibit a Region-1 sense, i.e. a downward FAC lying eastward of an upward FAC, and can close through Pedersen currents in the ionosphere, implying that the locally enhanced dawn-dusk electric field across the patch is imposed by processes in the magnetosphere. In FAST data, we identify localized precipitation that is enhanced within patches in comparison to weak polar rain outside patches. The precipitation consists of structured or diffuse soft electron fluxes. While the latter resembles polar rain only with higher fluxes, the former consists of discrete fluxes enhanced by 1-2 orders of magnitude from several to several hundred eV. Although the precipitation is not a major contributor to patch ionization, it implies that newly reconnected flux tubes that retain electrons of magnetosheath origin can rapidly traverse the polar cap from the dayside. Therefore non-storm time patches should be regarded as part of a localized magnetosphere-ionosphere coupling system along open magnetic field lines, and their transpolar evolution as a reflection of reconnected flux tubes traveling from the dayside to nightside magnetosphere.

Active role of fatty acid amino acid conjugates in nitrogen metabolism in Spodoptera litura larvae

PubMed Central

Yoshinaga, Naoko; Aboshi, Takako; Abe, Hiroaki; Nishida, Ritsuo; Alborn, Hans T.; Tumlinson, James H.; Mori, Naoki

2008-01-01

Since the first fatty acid amino acid conjugate (FAC) was isolated from regurgitant of Spodoptera exigua larvae in 1997 [volicitin: N-(17-hydroxylinolenoyl)-l-glutamine], their role as elicitors of induced responses in plants has been well documented. However, studies of the biosyntheses and the physiological role of FACs in the insect have been minimal. By using 14C-labeled glutamine, glutamic acid, and linolenic acid in feeding studies of Spodoptera litura larvae, combined with tissue analyses, we found glutamine in the midgut cells to be a major source for biosynthesis of FACs. Furthermore, 20% of the glutamine moiety of FACs was derived from glutamic acid and ammonia through enzymatic reaction of glutamine synthetase (GS). To determine whether FACs improve GS productivity, we studied nitrogen assimilation efficiency of S. litura larvae fed on artificial diets containing 15NH4Cl and glutamic acid. When the diet was enriched with linolenic acid, the nitrogen assimilation efficiency improved from 40% to >60%. In the lumen, the biosynthesized FACs are hydrolyzed to fatty acids and glutamine, which are reabsorbed into tissues and hemolymph. These results strongly suggested that FACs play an active role in nitrogen assimilation in Lepidoptera larva and that glutamine containing FACs in the gut lumen may function as a form of storage of glutamine, a key compound of nitrogen metabolism. PMID:18997016

48 CFR 301.603-74 - Requirement for retention of FAC-C and HHS SAC certification.

Code of Federal Regulations, 2010 CFR

2010-10-01

... System HEALTH AND HUMAN SERVICES GENERAL HHS ACQUISITION REGULATION SYSTEM Career Development... result in a loss of warrant authority. (Note: The certification programs' continuous learning requirement...

Development of the monitoring system to detect the piping thickness reduction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, N. Y.; Ryu, K. H.; Oh, Y. J.

2006-07-01

As nuclear piping becomes aging, secondary piping which was considered safe, undergo thickness reduction problem these days. After some accidents caused by Flow Accelerated Corrosion (FAC), guidelines and recommendations for the thinned pipe management were issued, and thus need for monitoring increases. Through thinned pipe management program, monitoring activities based on the various analyses and the case study of other plants also increases. As the monitoring points increase, time needs to cover the recommended inspection area becomes increasing, while the time given to inspect the piping during overhaul becomes shortened. Existing Ultrasonic Technique (UT) can cover small area in amore » given time. Moreover, it cannot be applied to a complex geometry piping or a certain location like welded part. In this paper, we suggested Switching Direct Current Potential Drop (S-DCPD) method by which we can narrow down the FAC-susceptible area. To apply DCPD, we developed both resistance model and Finite Element Method (FEM) model to predict the DCPD feasibility. We tested elbow specimen to compare DCPD monitoring results with UT results to identify consistency. For the validation test, we designed simulation loop. To determine the text condition, we analyzed environmental parameters and introduced applicable wearing rate model. To obtain the model parameters, we developed electrodes and analyzed velocity profile in the test loop using CFX code. Based on the prediction model and prototype testing results, we are planning to perform validation test to identify applicability of S-DCPD in the NPP environment. Validation text plan will be described as a future work. (authors)« less

Comparison of flow cytometry and immunohistochemistry in non-radioisotopic murine lymph node assay using bromodeoxyuridine.

PubMed

Jung, Kyoung-Mi; Bae, Il-Hong; Kim, Bae-Hwan; Kim, Wang-Ki; Chung, Jin-Ho; Park, Young-Ho; Lim, Kyung-Min

2010-02-01

Non-radioisotopic local lymph node assay (LLNA) employing 5-bromo-2'-deoxyuridine (BrdU) with flow cytometry (FACS) or immunohistochemistry (IHC) is gaining attention due to a regulatory issue of using radioisotope, (3)H-thymidine, in vivo in traditional LLNA. In this study, to compare the performance of these non-radioisotopic endpoints, 7 chemicals with known sensitizing potencies were examined in LLNA. Mice were topically treated with chemicals or vehicle on both ears for 3 days. After intraperitoneal injection of BrdU, bilateral lymph nodes were isolated separately and undergone respectively, FACS or IHC to determine BrdU incorporated lymph node cells (LNCs). Weight and histology of treated ears were also examined to evaluate chemical-induced edema and irritation. Both FACS and IHC could successively identify the skin sensitizers from non-sensitizers. Comparison of FACS and IHC with traditional LLNA revealed that FACS has a higher sensitivity although both assays produced comparable sensitivity and performance to traditional LLNA. In conclusion, non-radioisotopic LLNA using FACS and IHC can successfully detect sensitizers with a good correlation to traditional LLNA. Notably, FACS showed almost equivalent sensitivity and accuracy to traditional LLNA. 2009 Elsevier Ireland Ltd. All rights reserved.

Filamentary field-aligned currents at the polar cap region during northward interplanetary magnetic field derived with the Swarm constellation

PubMed Central

Lühr, Hermann; Huang, Tao; Wing, Simon; Kervalishvili, Guram; Rauberg, Jan; Korth, Haje

2017-01-01

ESA’s Swarm constellation mission makes it possible for the first time to determine field-aligned currents (FACs) in the ionosphere uniquely. In particular at high latitudes, the dual-satellite approach can reliably detect some FAC structures which are missed by the traditional single-satellite technique. These FAC events occur preferentially poleward of the auroral oval and during times of northward interplanetary magnetic field (IMF) orientation. Most events appear on the nightside. They are not related to the typical FAC structures poleward of the cusp, commonly termed NBZ. Simultaneously observed precipitating particle spectrograms and auroral images from Defense Meteorological Satellite Program (DMSP) satellites are consistent with the detected FACs and indicate that they occur on closed field lines mostly adjacent to the auroral oval. We suggest that the FACs are associated with Sun-aligned filamentary auroral arcs. Here we introduce in an initial study features of the high-latitude FAC structures which have been observed during the early phase of the Swarm mission. A more systematic survey over longer times is required to fully characterize the so far undetected field aligned currents. PMID:29056833

The Fear-Avoidance Components Scale (FACS): Responsiveness to Functional Restoration Treatment in a Chronic Musculoskeletal Pain Disorder (CMPD) Population.

PubMed

Neblett, Randy; Mayer, Tom G; Williams, Mark J; Asih, Sali; Cuesta-Vargas, Antonio I; Hartzell, Meredith M; Gatchel, Robert J

2017-12-01

To assess the clinical validity and factor structure of the Fear-Avoidance Components Scale (FACS), a new fear-avoidance measure. In this study, 426 chronic musculoskeletal pain disorder patients were admitted to a Functional Restoration Program (FRP). They were categorized into 5 FACS severity levels, from subclinical to extreme, at admission, and again at discharge. Associations with objective lifting performance and other patient-reported psychosocial measures were determined at admission and discharge, and objective work outcomes for this predominantly disabled cohort, were assessed 1 year later. Those patients in the severe and extreme FACS severity groups at admission were more likely to "drop out" of treatment than those in the lower severity groups (P=0.05). At both admission and discharge, the FACS severity groups were highly and inversely correlated with objective lifting performance and patient-reported fear-avoidance-related psychosocial variables, including kinesiophobia, pain intensity, depressive symptoms, perceived disability, perceived injustice, and insomnia (Ps<0.001). All variables showed improvement at FRP discharge. Patients in the extreme FACS severity group at discharge were less likely to return to, or retain, work 1 year later (P≤0.02). A factor analysis identified a 2-factor solution. Strong associations were found among FACS scores and other patient-reported psychosocial and objective lifting performance variables at both admission and discharge. High discharge-FACS scores were associated with worse work outcomes 1 year after discharge. The FACS seems to be a valid and clinically useful measure for predicting attendance, physical performance, distress, and relevant work outcomes in FRP treatment of chronic musculoskeletal pain disorder patients.

Comparison of cosmetic outcomes of absorbable versus nonabsorbable sutures in pediatric facial lacerations.

PubMed

Luck, Raemma; Tredway, Trevor; Gerard, James; Eyal, Dalit; Krug, Lauren; Flood, Robert

2013-06-01

We sought to compare cosmetic outcomes, complication rates, and patient/caregiver satisfaction of absorbable versus nonabsorbable sutures in children. Healthy patients, 1 to 18 years old, with facial lacerations 1 to 5 cm, were randomized to repair with fast-absorbing catgut (FAC) or nylon (NYL) sutures. Patients returned in 4 to 7 days and in 3 to 4 months, at which time photographs and caregiver surveys were completed. Unlike part I, all FAC sutures were permitted to absorb rather than be removed. Using a 100-mm visual analog scale (VAS), a noninferiority (NI) design was applied, with a difference of less than 15 mm considered clinically equivalent. Caregivers and 3 blinded physicians independently rated the scars via photographs. Ninety-eight patients were enrolled, 76 caregiver surveys were completed, and 61 (29 FAC, 32 NYL) had photographs scored by physicians. The mean physician VAS scores for FAC and NYL were 57.6 and 67.6, respectively (difference, -10.0; 95% confidence interval, -19.1 to -0.4); thus, NI could not be established. The mean caregiver VAS scores for the FAC and NYL groups were 93.8 and 86.6, respectively (difference, 7.2; 95% confidence interval, -4.9 to 13.9); thus, NI of FAC was established. There were no significant differences in rates of infection, wound dehiscence, or keloid formation. In terms of future preference, caregivers favored FAC (33/33) over NYL (26/36) (P < 0.01). Caregiver VAS scores showed NI of FAC, which were also preferred by the caregivers. However, NI for FAC could not be demonstrated by blinded physicians with respect to cosmetic outcomes.

The Fanconi anemia pathway requires FAA phosphorylation and FAA/FAC nuclear accumulation

PubMed Central

Yamashita, Takayuki; Kupfer, Gary M.; Naf, Dieter; Suliman, Ahmed; Joenje, Hans; Asano, Shigetaka; D’Andrea, Alan D.

1998-01-01

Fanconi anemia (FA) is an autosomal recessive cancer susceptibility syndrome with at least eight complementation groups (A–H). Two FA genes, corresponding to complementation groups A and C, have been cloned, but the function of the FAA and FAC proteins remains unknown. We have recently shown that the FAA and FAC proteins bind and form a nuclear complex. In the current study, we analyzed the FAA and FAC proteins in normal lymphoblasts and lymphoblasts from multiple FA complementation groups. In contrast to normal controls, FA cells derived from groups A, B, C, E, F, G, and H were defective in the formation of the FAA/FAC protein complex, the phosphorylation of the FAA protein, and the accumulation of the FAA/FAC protein complex in the nucleus. These biochemical events seem to define a signaling pathway required for the maintenance of genomic stability and normal hematopoiesis. Our results support the idea that multiple gene products cooperate in the FA Pathway. PMID:9789045

NLTE opacity calculations: C-Si and C-Ge mixtures

NASA Astrophysics Data System (ADS)

Jarrah, W.; Benredjem, D.; Pain, J.-C.; Dubau, J.

2017-09-01

The opacity is an important issue in the knowledge of the radiative properties of ICF and astrophysical plasmas. We present the opacity of dopants (silicon, germanium) embedded in the ablator of some ICF capsules. In recent works, Hill and Rose calculated the opacity of silicon in LTE and non-LTE plasmas, while Minguez and co-workers focused on the opacity of carbon. We have used the Cowan code to calculate the atomic structure of carbon, silicon and germanium in various ionic stages. The cross-sections of atomic processes (collisional excitation, collisional ionization) are obtained by fitting the values given by the code FAC to the Van Regemorter-like formulas of Sampson and Zhang. A corrected Gaunt factor is then obtained. A collisional-radiative code was developed in order to obtain the ionic populations, the level populations and the opacity. Line broadening and line shift are taken into account. The ionization potential depression is included in our calculations. The effect of a radiation field on the opacity is examined.

Tuning the reactivity in classic low-spin d6 rhenium(I) tricarbonyl radiopharmaceutical synthon by selective bidentate ligand variation (L,L'-Bid; L,L'= N,N', N,O, and O,O' donor atom sets) in fac-[Re(CO)3(L,L'-Bid)(MeOH)]n complexes.

PubMed

Schutte, Marietjie; Kemp, Gerdus; Visser, Hendrik G; Roodt, Andreas

2011-12-19

A range of fac-[Re(CO)(3)(L,L'-Bid)(H(2)O)](n) (L,L'-Bid = neutral or monoanionic bidentate ligands with varied L,L' donor atoms, N,N', N,O, or O,O': 1,10-phenanthroline, 2,2'-bipydine, 2-picolinate, 2-quinolinate, 2,4-dipicolinate, 2,4-diquinolinate, tribromotropolonate, and hydroxyflavonate; n = 0, +1) has been synthesized and the aqua/methanol substitution has been investigated. The complexes were characterized by UV-vis, IR and NMR spectroscopy and X-ray crystallographic studies of the compounds fac-[Re(CO)(3)(Phen)(H(2)O)]NO(3)·0.5Phen, fac-[Re(CO)(3)(2,4-dQuinH)(H(2)O)]·H(2)O, fac-[Re(CO)(3)(2,4-dQuinH)Py]Py, and fac-[Re(CO)(3)(Flav)(CH(3)OH)]·CH(3)OH are reported. A four order-of-magnitude of activation for the methanol substitution is induced as manifested by the second order rate constants with (N,N'-Bid) < (N,O-Bid) < (O,O'-Bid). Forward and reverse rate and stability constants from slow and stopped-flow UV/vis measurements (k(1), M(-1) s(-1); k(-1), s(-1); K(1), M(-1)) for bromide anions as entering nucleophile are as follows: fac-[Re(CO)(3)(Phen)(MeOH)](+) (50 ± 3) × 10(-3), (5.9 ± 0.3) × 10(-4), 84 ± 7; fac-[Re(CO)(3)(2,4-dPicoH)(MeOH)] (15.7 ± 0.2) × 10(-3), (6.3 ± 0.8) × 10(-4), 25 ± 3; fac-[Re(CO)(3)(TropBr(3))(MeOH)] (7.06 ± 0.04) × 10(-2), (4 ± 1) × 10(-3), 18 ± 4; fac-[Re(CO)(3)(Flav)(MeOH)] 7.2 ± 0.3, 3.17 ± 0.09, 2.5 ± 2. Activation parameters (ΔH(k1)(++), kJmol(-1); ΔS(k1)(), J K(-1) mol(-1)) from Eyring plots for entering nucleophiles as indicated are as follows: fac-[Re(CO)(3)(Phen)(MeOH)](+) iodide 70 ± 1, -35 ± 3; fac-[Re(CO)(3)(2,4-dPico)(MeOH)] bromide 80.8 ± 6, -8 ± 2; fac-[Re(CO)(3)(Flav)(MeOH)] bromide 52 ± 5, -52 ± 15. A dissociative interchange mechanism is proposed. © 2011 American Chemical Society

Multiscale field-aligned current analyzer

NASA Astrophysics Data System (ADS)

Bunescu, C.; Marghitu, O.; Constantinescu, D.; Narita, Y.; Vogt, J.; Blǎgǎu, A.

2015-11-01

The magnetosphere-ionosphere coupling is achieved, essentially, by a superposition of quasi-stationary and time-dependent field-aligned currents (FACs), over a broad range of spatial and temporal scales. The planarity of the FAC structures observed by satellite data and the orientation of the planar FAC sheets can be investigated by the well-established minimum variance analysis (MVA) of the magnetic perturbation. However, such investigations are often constrained to a predefined time window, i.e., to a specific scale of the FAC. The multiscale field-aligned current analyzer, introduced here, relies on performing MVA continuously and over a range of scales by varying the width of the analyzing window, appropriate for the complexity of the magnetic field signatures above the auroral oval. The proposed technique provides multiscale information on the planarity and orientation of the observed FACs. A new approach, based on the derivative of the largest eigenvalue of the magnetic variance matrix with respect to the length of the analysis window, makes possible the inference of the current structures' location (center) and scale (thickness). The capabilities of the FAC analyzer are explored analytically for the magnetic field profile of the Harris sheet and tested on synthetic FAC structures with uniform current density and infinite or finite geometry in the cross-section plane of the FAC. The method is illustrated with data observed by the Cluster spacecraft on crossing the nightside auroral region, and the results are cross checked with the optical observations from the Time History of Events and Macroscale Interactions during Substorms ground network.

Effect of sleep deprivation on driving safety in housestaff.

PubMed

Marcus, C L; Loughlin, G M

1996-12-01

Sleep deprivation is known to affect driving safety. Housestaff (HS) are routinely sleep-deprived when on call. We hypothesized that this would affect their driving. We therefore administered questionnaires regarding driving to 70 pediatric HS, who were on call every fourth night, and to 85 faculty members (FAC), who were rarely disturbed at night. HS were questioned about events during their residency, and FAC were questioned about events during the preceding three years. There was an 87% response rate for each group. HS slept 2.7 +/- 0.9 (SD) hours when on call vs 7.2 +/- 0.8 hours when not on call (p < 0.001). 44% of HS had fallen asleep when stopped at a light, vs 12.5% FAC (p < 0.001). 23% of HS had fallen asleep while driving vs. 8% FAC (ns). A total of 49% of HS had fallen asleep at the wheel; 90% of these events occurred post-call. In contrast, only 13% of FAC had fallen asleep at the wheel (p < 0.001). HS had received a total of 25 traffic citations for moving violations vs. 15 for FAC and were involved in 20 motor vehicle accidents vs. 11 for FAC. One traffic citation clearly resulted from HS falling asleep at the wheel vs. none for FAC. We conclude that HS frequently fall asleep when driving post-call. We speculate that current HS work schedules may place some HS at risk for injury to themselves and others. Further study, using prospectively objective measures is indicated.

The Poleward Boundary Intensification (PBI) of Auroral Emission: Its Dynamics and Associated Field-aligned Current System

NASA Astrophysics Data System (ADS)

Ohtani, S.; Motoba, T.; Gjerloev, J. W.

2016-12-01

The poleward boundary intensification (PBI) of aurora emission is often addressed in terms of distant reconnection. Recently, however, Ohtani and Yoshikawa [2016] proposed that the PBIs, at least at the initial stage of their formation, are actually the effect of ionospheric polarization in the presence of the enhanced convection in the polar cap and conductance gradient at the poleward boundary of the auroral oval. Whereas the ionospheric polarization itself is a transient process, it is known that the PBIs occasionally extend longitudinally suggesting that a 3D current system forms subsequently, which electrodynamically couples the magnetosphere and ionosphere. In the present study we observationally examine the associated field-aligned current (FAC) observed by the SWARM satellites and compare its characteristics with ground all-sky images. It is found that complex signatures of FACs as suggested by magnetic disturbances reflect the spatial structure of aurora (e.g., location and orientation), whereas the overall motion of PBIs is well explained in terms of the background convection suggested by the FAC distribution. We shall discuss the implications of these results for the responsible evolution process of the PBIs.

Energy levels and radiative rates for transitions in Cr-like Co IV and Ni V

NASA Astrophysics Data System (ADS)

Aggarwal, K. M.; Bogdanovich, P.; Karpuškienė, R.; Keenan, F. P.; Kisielius, R.; Stancalie, V.

2016-01-01

We report calculations of energy levels and radiative rates (A-values) for transitions in Cr-like Co IV and Ni V. The quasi-relativistic Hartree-Fock (QRHF) code is adopted for calculating the data although GRASP (general-purpose relativistic atomic structure package) and flexible atomic code (FAC) have also been employed for comparison purposes. No radiative rates are available in the literature to compare with our results, but our calculated energies are in close agreement with those compiled by NIST for a majority of the levels. However, there are discrepancies for a few levels of up to 3%. The A-values are listed for all significantly contributing E1, E2 and M1 transitions, and the corresponding lifetimes reported, although unfortunately no previous theoretical or experimental results exist to compare with our data.

Interim Regional Supplement to the Corps of Engineers Wetland Delineation Manual: Great Plans Region

DTIC Science & Technology

2008-03-01

artemisiifolia Iva annua Cardiospermum halicacabum Xanthium strumarium FACW FACU− FAC FAC FAC− 40 15 15 8 6 Yes Yes Yes No No Total...virgatum 50 40 90 2 180 FAC species Iva annua Celtis laevigata Cardiospermum halicacabum Xanthium strumarium Toxicodendron radicans3 15 12

48 CFR 301.607-74 - Certification transfers.

Code of Federal Regulations, 2012 CFR

2012-10-01

....607-74 Certification transfers. (a) HHS recognizes and accepts FAC-P/PM certifications issued by other... under FAC-P/PM. See FAI's Web site, and Chapter 3, Federal Acquisition Certification—Program and Project... certification transfer should not be initiated when an individual, who holds a current FAC-P/PM certification...

48 CFR 301.607-74 - Certification transfers.

Code of Federal Regulations, 2013 CFR

2013-10-01

....607-74 Certification transfers. (a) HHS recognizes and accepts FAC-P/PM certifications issued by other... under FAC-P/PM. See FAI's Web site, and Chapter 3, Federal Acquisition Certification—Program and Project... certification transfer should not be initiated when an individual, who holds a current FAC-P/PM certification...

48 CFR 301.607-74 - Certification transfers.

Code of Federal Regulations, 2014 CFR

2014-10-01

....607-74 Certification transfers. (a) HHS recognizes and accepts FAC-P/PM certifications issued by other... under FAC-P/PM. See FAI's Web site, and Chapter 3, Federal Acquisition Certification—Program and Project... certification transfer should not be initiated when an individual, who holds a current FAC-P/PM certification...

48 CFR 301.607-74 - Certification transfers.

Code of Federal Regulations, 2011 CFR

2011-10-01

....607-74 Certification transfers. (a) HHS recognizes and accepts FAC-P/PM certifications issued by other... under FAC-P/PM. See FAI's Web site, and Chapter 3, Federal Acquisition Certification—Program and Project... certification transfer should not be initiated when an individual, who holds a current FAC-P/PM certification...

Antifungal Susceptibility Testing of Fluconazole by Flow Cytometry Correlates with Clinical Outcome

PubMed Central

Wenisch, Christoph; Moore, Caroline B.; Krause, Robert; Presterl, Elisabeth; Pichna, Peter; Denning, David W.

2001-01-01

Susceptibility testing of fungi by flow cytometry (also called fluorescence-activated cell sorting [FACS]) using vital staining with FUN-1 showed a good correlation with the standard M27-A procedure for assessing MICs. In this study we determined MICs for blood culture isolates from patients with candidemia by NCCLS M27-A and FACS methods and correlated the clinical outcome of these patients with in vitro antifungal resistance test results. A total of 24 patients with candidemia for whom one or more blood cultures were positive for a Candida sp. were included. Susceptibility testing was performed by NCCLS M27-A and FACS methods. The correlation of MICs (NCCLS M27-A and FACS) and clinical outcome was calculated. In 83% of the cases, the MICs of fluconazole determined by FACS were within 1 dilution of the MICs determined by the NCCLS M27-A method. For proposed susceptibility breakpoints, there was 100% agreement between the M27-A and FACS methods. In the FACS assay, a fluconazole MIC of <1 μg/ml was associated with cure (P < 0.001) whereas an MIC of ≥1 μg/ml was associated with death (P < 0.001). The M27-A-derived fluconazole MICs did not correlate with outcome (P = 1 and P = 0.133). PMID:11427554

Chexal-Horowitz flow-accelerated corrosion model -- Parameters and influences

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chexal, V.K.; Horowitz, J.S.

1995-12-01

Flow-accelerated corrosion (FAC) continues to cause problems in nuclear and fossil power plants. Thinning caused by FAC has lead to many leaks and complete ruptures. These failures have required costly repairs and occasionally have caused lengthy shutdowns. To deal with FAC, utilities have instituted costly inspection and piping replacement programs. Typically, a nuclear unit will inspect about 100 large bore piping components plus additional small bore components during every refueling outage. To cope with FAC, there has been a great deal of research and development performed to obtain a greater understanding of the phenomenon. Currently, there is general agreement onmore » the mechanism of FAC. This understanding has lead to the development of computer based tools to assist utility engineers in dealing with this issue. In the United States, the most commonly used computer program to predict and control is CHECWORKS{trademark}. This paper presents a description of the mechanism of FAC, and introduces the predictive algorithms used in CHECWORKS{trademark}. The parametric effects of water chemistry, materials, flow and geometry as predicted by CHECWORKS{trademark} will then be discussed. These trends will be described and explained by reference to the corrosion mechanism. The remedial actions possible to reduce the rate of damage caused by FAC will also be discussed.« less

Defense Meteorological Satellite Program Data in Dynamic Auroral Boundary Coordinates: New insights into Polar Cap and Auroral Dynamics

NASA Astrophysics Data System (ADS)

Knipp, D.

2016-12-01

Using reprocessed (Level-2) data from the Defense Meteorology Satellite Program magnetometer (SSM) and particle precipitation (SSJ) instruments we determine the boundaries of the central plasma sheet auroral oval, and then consider the relative locations and intensities of field aligned currents. Large-scale field-aligned currents (FAC) are determined using the Minimum Variance Analysis technique, and their influence is then removed from the magnetic perturbations allowing us to estimate intensity and scale-size of the smaller-scale currents. When sorted by dynamic auroral boundary coordinates we find that large- scale Region 1 (R1) FAC are often within the polar cap and Region 2 (R2) FAC show a strong dawn-dusk asymmetry (as in Ohtani et al., 2010). We find that mesoscale FAC are stronger in the summer and are most consistently present in the vicinity of dawnside (downward) R1 FAC. Further, mesoscale FAC are confined to auroral latitudes and above on the dawnside, but can be subaroural on the dusk side. Hotspots of mesoscale FAC occur in pre-midnight regions especially during summer. Finally, we show how this information can be combined with measurements from above and below the ionosphere-thermosphere to help explain significant perturbations in polar cap dynamics.

A Focus Group Assessment to Determine Motivations, Barriers and Effectiveness of a University-Based Worksite Wellness Program

PubMed Central

Hill-Mey, Patricia E.; Merrill, Ray M.; Kumpfer, Karol L.; Reel, Justine; Hyatt-Neville, Beverly

2013-01-01

Background: This study explores university employee perceptions and under­standing about its Worksite Health Promotion Program (WHPP). The WHPP included a Health Risk Appraisal (HRA), biometric screening, publicity for on-campus health programs and facilities, and health coaching. Methods: A qualitative design was used based on a grounded theory ap­proach. Four 90 minutes focus groups with 6-8 participants in each were conducted within a two 2 week period among employees, representing fac­ulty/participants, fac­ulty/nonparticipants, staff/participants, and staff/nonparticipants. Responses to questions about motivations, barriers, and perceived health benefits that impacted participation in the WHPP were digi­tally recorded, transcribed and coded for themes. Results: Incentives effectively motivated participation. Biometric screening had the largest impact on behavior change, followed by the information learned from the HRA. However, despite two-thirds of the employees partici­pating in the pro­gram, lack of a full understanding of WHPP benefits and services lowered partici­pation in follow-up services and supplemental pro­grams. Conclusions: Biometric screening and HRAs effectively motivate program par­ticipation. Communication of benefits and services are important when providing WHPPs. PMID:24688965

Dielectronic recombination of lowly charged tungsten ions Wq+(q = 5 - 10)

NASA Astrophysics Data System (ADS)

Kwon, Duck-Hee

2018-03-01

Dielectronic recombination (DR) rate coefficients for the ground levels of low ionization state Wq+ (q = 5 - 10) ions have been obtained by an ab-inito level-by-level calculation using the flexible atomic code (FAC) based on relativistic jj coupling scheme and independent process, isolated resonance, distorted wave approximation. The radiative transition calculation in the original FAC has been adapted into parallel programming for time effective dealing with so many resonance levels of the complex open 4f, 5p, or 5d-shell structure ion. Core excitations Δnc = 0 , 1 of 4f, 5p, and 5d (W5+), Δnc = 2 of 4f, and Δnc = 0 of 4d (W7+), and 5s (W8+) are included to the total DR rate coefficient. The core excitations Δnc = 0 , 5p → 5l and Δnc = 1 , 4f → 5l mainly contribute to the total DR rate coefficients. The strong resonances involved in the DR are analyzed and the total DR rate coefficients are compared with available previous ab-initio predictions and with ADAS data by a simple semiempirical formula.

Strong ionospheric field-aligned currents for radial interplanetary magnetic fields

NASA Astrophysics Data System (ADS)

Wang, Hui; Lühr, Hermann; Shue, Jih-Hong; Frey, Harald. U.; Kervalishvili, Guram; Huang, Tao; Cao, Xue; Pi, Gilbert; Ridley, Aaron J.

2014-05-01

The present work has investigated the configuration of field-aligned currents (FACs) during a long period of radial interplanetary magnetic field (IMF) on 19 May 2002 by using high-resolution and precise vector magnetic field measurements of CHAMP satellite. During the interest period IMF By and Bz are weakly positive and Bx keeps pointing to the Earth for almost 10 h. The geomagnetic indices Dst is about -40 nT and AE about 100 nT on average. The cross polar cap potential calculated from Assimilative Mapping of Ionospheric Electrodynamics and derived from DMSP observations have average values of 10-20 kV. Obvious hemispheric differences are shown in the configurations of FACs on the dayside and nightside. At the south pole FACs diminish in intensity to magnitudes of about 0.1 μA/m2, the plasma convection maintains two-cell flow pattern, and the thermospheric density is quite low. However, there are obvious activities in the northern cusp region. One pair of FACs with a downward leg toward the pole and upward leg on the equatorward side emerge in the northern cusp region, exhibiting opposite polarity to FACs typical for duskward IMF orientation. An obvious sunward plasma flow channel persists during the whole period. These ionospheric features might be manifestations of an efficient magnetic reconnection process occurring in the northern magnetospheric flanks at high latitude. The enhanced ionospheric current systems might deposit large amount of Joule heating into the thermosphere. The air densities in the cusp region get enhanced and subsequently propagate equatorward on the dayside. Although geomagnetic indices during the radial IMF indicate low-level activity, the present study demonstrates that there are prevailing energy inputs from the magnetosphere to both the ionosphere and thermosphere in the northern polar cusp region.

Synthesis and characterization of fac-[M(CO)3(P)(OO)] and cis-trans-[M(CO)2(P)2(OO)] complexes (M = Re, (99m)Tc) with acetylacetone and curcumin as OO donor bidentate ligands.

PubMed

Triantis, Charalampos; Tsotakos, Theodoros; Tsoukalas, Charalampos; Sagnou, Marina; Raptopoulou, Catherine; Terzis, Aris; Psycharis, Vassilis; Pelecanou, Maria; Pirmettis, Ioannis; Papadopoulos, Minas

2013-11-18

The synthesis and characterization of neutral mixed ligand complexes fac-[M(CO)3(P)(OO)] and cis-trans-[M(CO)2(P)2(OO)] (M = Re, (99m)Tc), with deprotonated acetylacetone or curcumin as the OO donor bidentate ligands and a phosphine (triphenylphosphine or methyldiphenylphosphine) as the monodentate P ligand, is described. The complexes were synthesized through the corresponding fac-[M(CO)3(H2O)(OO)] (M = Re, (99m)Tc) intermediate aqua complex. In the presence of phosphine, replacement of the H2O molecule of the intermediate complex at room temperature generates the neutral tricarbonyl monophosphine fac-[Re(CO)3(P)(OO)] complex, while under reflux conditions further replacement of the trans to the phosphine carbonyl generates the new stable dicarbonyl bisphosphine complex cis-trans-[Re(CO)2(P)2(OO)]. The Re complexes were fully characterized by elemental analysis, spectroscopic methods, and X-ray crystallography showing a distorted octahedral geometry around Re. Both the monophosphine and the bisphosphine complexes of curcumin show selective binding to β-amyloid plaques of Alzheimer's disease. At the (99m)Tc tracer level, the same type of complexes, fac-[(99m)Tc(CO)3(P)(OO)] and cis-trans-[(99m)Tc(CO)2(P)2(OO)], are formed introducing new donor combinations for (99m)Tc(I). Overall, β-diketonate and phosphine constitute a versatile ligand combination for Re(I) and (99m)Tc(I), and the successful employment of the multipotent curcumin as β-diketone provides a solid example of the pharmacological potential of this system.

X-ray Spectroscopy of E2 and M3 Transitions in Ni-like W

DOE Office of Scientific and Technical Information (OSTI.GOV)

Clementson, J; Beiersdorfer, P; Gu, M F

2009-11-09

The electric quadrupole (E2) and magnetic octupole (M3) ground state transitions in Ni-like W{sup 46+} have been measured using high-resolution crystal spectroscopy at the Livermore electron beam ion trap facility. The lines fall in the soft x-ray region near 7.93 {angstrom} and were originally observed as an unresolved feature in tokamak plasmas. Using flat ADP and quartz crystals the wavelengths, intensities, and polarizations of the two lines have been measured for various electron beam energies and compared to intensity and polarization calculations performed using the Flexible Atomic Code (FAC).

Dielectronic recombination of the 4p and 4d open sub-shell tungsten ions

NASA Astrophysics Data System (ADS)

Li, M. J.; Fu, Y. B.; Zhang, G. D.; Zhang, Y. Z.; Dong, C. Z.; Koike, F.

2014-04-01

Dielectronic recombination rate coefficients are given theoretically for several highly charged tungsten ions. As 4p open sub-shell ions, Ga-, Ge-, As-, Br-, Kr-like ions are considered. Rb-like ion is further considered as a 4d open sub-shell ion. Theoretical calculations are carried out using a relativistic atomic code FAC. The effect of configuration interaction is taking into account. Inner-shell electron excitations play a significant role for the dielectronic recombination process. Simple analytical formulae are given for the total rate coefficients by fitting to the presently obtained numerical results.

Activation of rhenium(I) toward substitution in fac-[Re(N,O'-Bid)(CO)3(HOCH3)] by Schiff-base bidentate ligands (N,O'-Bid).

PubMed

Brink, Alice; Visser, Hendrik G; Roodt, Andreas

2013-08-05

A series of fac-[Re(N,O'-Bid)(CO)3(L)] (N,O'-Bid = monoanionic bidentate Schiff-base ligands with N,O donor atoms; L = neutral monodentate ligand) has been synthesized, and the methanol substitution reactions have been investigated. The complexes were characterized by NMR, IR, and UV-vis spectroscopy. X-ray crystal structures of the compounds fac-[Re(Sal-mTol)(CO)3(HOCH3)], fac-[Re(Sal-pTol)(CO)3(HOCH3)], fac-[Re(Sal-Ph)(CO)3(HOCH3)], and fac-[Re(Sal-Ph)(CO)3(Py)] (Sal-mTol = 2-(m-tolyliminomethyl)phenolato; Sal-pTol = 2-(p-tolyliminomethyl)phenolato; Sal-Ph = 2-(phenyliminomethyl)phenolato; Py = pyridine) are reported. Significant activation for the methanol substitution is induced by the use of the N,O bidentate ligand as manifested by the second order rate constants, with limiting kinetics being observed for the first time. Rate constants (25 °C) (k1 or k3) and activation parameters (ΔHk‡, kJ mol(-1); ΔSk‡, J K(-1) mol(-1)) from Eyring plots for entering nucleophiles as indicated are as follows: fac-[Re(Sal-mTol)(CO)3(HOCH3)] 3-chloropyridine: (k1) 2.33 ± 0.01 M(-1) s(-1); 85.1 ± 0.6, 48 ± 2; fac-[Re(Sal-mTol)(CO)3(HOCH3)] pyridine: (k1) 1.29 ± 0.02 M(-1) s(-1); 92 ± 2, 66 ± 7; fac-[Re(Sal-mTol)(CO)3(HOCH3)] 4-picoline: (k1) 1.27 ± 0.05 M(-1) s(-1); 88 ± 2, 53 ± 6; (k3) 3.9 ± 0.03 s(-1); 78 ± 8, 30 ± 27; (kf) 1.7 ± 0.02 M(-1) s(-1); 86 ± 2, 49 ± 6; fac-[Re(Sal-mTol)(CO)3(HOCH3)] DMAP (k3) 1.15 ± 0.02 s(-1); 88 ± 2, 52 ± 7. An interchange dissociative mechanism is proposed.

Several novel N-donor tridentate ligands formed in chemical studies of new fac-Re(CO)3 complexes relevant to fac-99mTc(CO)3 radiopharmaceuticals: attack of a terminal amine on coordinated acetonitrile.

PubMed

Perera, Theshini; Marzilli, Patricia A; Fronczek, Frank R; Marzilli, Luigi G

2010-03-01

To evaluate syntheses of fac-[Re(CO)(3)L](+) complexes in organic solvents, we treated fac-[Re(CO)(3)(CH(3)CN)(3)]PF(6)/BF(4) in acetonitrile with triamine ligands (L). When L had two primary or two tertiary terminal amine groups, the expected fac-[Re(CO)(3)L](+) complexes formed. In contrast, N,N-dimethyldiethylenetriamine (N,N-Me(2)dien) formed an unusual compound, fac-[Re(CO)(3)(DAE)]BF(4) {DAE = (Z)-N'-(2-(2-(dimethylamino)ethylamino)ethyl)acetimidamide = (Me(2)NCH(2)CH(2))NH(CH(2)CH(2)N=C(NH(2))Me)}. DAE is formed by addition of acetonitrile to the N,N-Me(2)dien terminal primary amine, converting this sp(3) nitrogen to an sp(2) nitrogen with a double bond to the original acetonitrile sp carbon. The three Ns bound to Re derive from N,N-Me(2)dien. The pathway to fac-[Re(CO)(3)(DAE)]BF(4) is suggested by a second unusual compound, fac-[Re(CO)(3)(MAE)]PF(6) {MAE = N-methyl-N-(2-(methyl-(2-(methylamino)ethyl)amino)ethyl)acetimidamide = MeN(H)-CH(2)CH(2)-N(Me)-CH(2)CH(2)-N(Me)-C(Me)=NH}, isolated after treating fac-[Re(CO)(3)(CH(3)CN)(3)]PF(6) with N,N',N''-trimethyldiethylenetriamine (N,N',N''-Me(3)dien). MAE chelates via a terminal and a central sp(3) N from N,N',N''-Me(3)dien and via one sp(2) NH in a C(Me)=NH group. This group is derived from acetonitrile by addition of the other N,N',N''-Me(3)dien terminal amine to the nitrile carbon. This addition creates an endocyclic NMe group within a seven-membered chelate ring. The structure and other properties of fac-[Re(CO)(3)(MAE)]PF(6) allow us to propose a reaction scheme for the formation of the unprecedented DAE ligand. The new compounds advance our understanding of the spectral and structural properties of Re analogues of (99m)Tc radiopharmaceuticals.

Coordinated aqua vs methanol substitution kinetics in fac-Re(I) tricarbonyl tropolonato complexes.

PubMed

Schutte, Marietjie; Roodt, Andreas; Visser, Hendrik G

2012-11-05

Water-soluble fac-[Re(CO)(3)(L,L'-Bid)(X)] (L,L'-Bid = tropolonato, X = H(2)O, methanol) complexes have been synthesized, and the aqua and methanol substitution reactions were investigated in water (pH range 6.3-10.0) and methanol, respectively, and compared. Thiocyanate ions were used as monodentate entering ligand. The complexes were characterized by UV-vis, IR, and NMR spectroscopy. The crystal structures of the complexes [NEt(4)] fac-[Re(Trop)(CO)(3)(H(2)O)].NO(3).H(2)O (reactant) and fac-[Re(CO)(3)(Trop)(Py)], a substitution product, are reported. Overall it was found that the aqua substitution of fac-[Re(CO)(3)(Trop)(H(2)O)] is about 10 times faster than the methanol substitution reaction for fac-[Re(CO)(3)(Trop)(MeOH)], with forward and reverse rate and stability constants [k(1) (M(-1) s(-1)), k(-1) (s(-1)), K(1), (M(-1))] for thiocyanate as monodentate entering ligand as follows: fac-[Re(CO)(3)(Trop)(H(2)O)] = 2.54 ± 0.03, 0.0077 ± 0.0005, 330 ± 22/207 ± 14 and fac-[Re(CO)(3)(Trop)(MeOH)] = 0.268 ± 0.002, 0.0044 ± 0.0002, (61 ± 3)/(52 ± 4). The activation parameters [ΔH(‡)(k1) (kJ mol(-1)), ΔS(‡)(k1) (J K(-1) mol(-1))] for the aqua and methanol complex respectively are 56.1 ± 0.7, -49 ± 2 and 64 ± 1, -43 ± 5.

Field-aligned Currents' Scale Analysis Performed by the Swarm Constellation

NASA Astrophysics Data System (ADS)

Luhr, H.; Park, J.; Gjerloev, J. W.; Rauberg, J.; Michaelis, I.; Le, G.; Merayo, J. M. G.; Brauer, P.

2014-12-01

We present a statistical study of the temporal and spatial scale characteristics of different field-aligned current (FAC) types. Very suitable for this purpose is the closely spaced Swarm satellite formation, which existed shortly after launch during the commissioning phase. As dataset we use the standard Level 2 product, Single Satellite FAC, which comes at a data rate of 1 Hz, corresponding to an along-track distance of 7.5 km. FACs are known to cover a wide range of scales from 1km to several hundred kilometres, the smaller the scale the larger the amplitude. We like to divide the FACs into two classes. Those of intermediate scale, some tens of kilometres, which are carried predominantly by kinetic Alfvén waves, while the large-scale FACs are assumed to be stationary current structures on the timescales of a satellite crossing. For distinguishing between the two we first look how the temporal variability changes with scale. For that we consider subsequent measurements at the same point, the orbital cross-over near the geographic poles, and interpret the temporal current changes. Here we focus on observations in the southern hemisphere at locations where the geographic pole lies within the auroral region. In a next step the latitudinal and longitudinal scales of the larger-scale FAC structures are investigated. FACs related to Alfvén waves cannot be studied in this way because we have no simultaneous measurements at the same latitude and longitude. The results from this analysis are different for dayside and nightside. Implications for the FAC characteristics resulting from these observations are interpreted in the end.

National Standards for Family and Consumer Sciences Education.

ERIC Educational Resources Information Center

National Association of State Administrators for Family and Consumer Sciences.

The document presents a new set of standards for family and consumer sciences (FACS) education. Section 1 is a three-chapter overview. Chapter 1 addresses the rationale for change and the FACS vision and mission. Chapter 2 describes the approach to develop the national standards, FACS format, and components of the standards. Chapter 3 provides…

Increased universality of Lepidopteran elicitor compounds across insects: Identification of fatty acid amino acid conjugates (FACs)

USDA-ARS?s Scientific Manuscript database

Fatty acid amino acid conjugates (FACs) are known elicitors of induced release of volatile compounds in plants that, in turn, attract foraging parasitoids. Since the discovery of volicitin [N-(17-hydroxylinolenoyl)-L-glutamine] in the regurgitant of larval Spodoptera exigua1, a series of related FAC...

75 FR 39420 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-44; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-08

... Federal Acquisition Circular (FAC) 2005-44 which amends the Federal Acquisition Regulation (FAR). Interested parties may obtain further information regarding this rule by referring to FAC 2005-44 which... . FOR FURTHER INFORMATION CONTACT: The analyst whose name appears in the table below. Please cite FAC...

2 CFR 200.513 - Responsibilities.

Code of Federal Regulations, 2014 CFR

2014-01-01

... notice of the change to the FAC, the auditee, and, if known, the auditor. The cognizant agency for audit... agency for audit must provide notice of the change to the FAC, the auditee, and, if known, the auditor...; providing input on single audit and follow-up policy; enhancing the utility of the FAC; and studying ways to...

76 FR 4191 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-49; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

... Federal Acquisition Circular (FAC) 2005-49, which amend the Federal Acquisition Regulation (FAR). Interested parties may obtain further information regarding these rules by referring to FAC 2005-49, which... analyst whose name appears in the table below. Please cite FAC 2005-49 and the specific FAR case number...

F-Area Acid/Caustic Basin groundwater monitoring report: Third quarter 1994

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1994-12-01

During third quarter 1994, samples from the FAC monitoring wells at the F-Area Acid/Caustic Basin were collected and analyzed for herbicides/pesticides, indicator parameters, metals, nitrate, radionuclide indicators, volatile organic compounds, and other constituents. Piezometer FAC 5P and monitoring well FAC 6 were dry and could not be sampled. New monitoring wells FAC 9C, 10C, 11C, and 12C were sampled for the first time during third quarter. Analytical results that exceeded final Primary Drinking Water Standards (PDWS), other Savannah River Site (SRS) Flag 2 criteria, or the SRS turbidity standard of 50 NTU during the quarter were as follows: gross alphamore » exceeded the final PDWS and aluminum, iron, manganese, and total alpha-emitting radium exceeded the SRS Flag 2 criteria in one or more of the FAC wells. Turbidity exceeded the SRS standard in wells FAC 3 and 10C. Groundwater flow direction and rate in the water table beneath the F-Area Acid/Caustic Basin were similar to past quarters.« less

Crystal structures of fac-tri-chlorido-tris-(tri-methyl-phosphane-κP)rhodium(III) monohydrate and fac-tri-chlorido-tris-(tri-methyl-phosphane-κP)rhodium(III) methanol hemisolvate: rhodium structures that are isotypic with their iridium analogs.

PubMed

Merola, Joseph S; Franks, Marion A

2015-02-01

The crystal structures of two solvates of fac-tri-chlorido-tris-(tri-methyl-phosphane-κP)rhodium(III) are reported, i.e. one with water in the crystal lattice, fac-[RhCl3(Me3P)3]·H2O, and one with methanol in the crystal lattice, fac-[RhCl3(Me3P)3]·0.5CH3OH. These rhodium compounds exhibit distorted octahedral coordination spheres at the metal and are isotypic with the analogous iridium compounds previously reported by us [Merola et al. (2013 ▶). Polyhedron, 54, 67-73]. Comparison is made between the rhodium and iridium compounds, highlighting their isostructural relationships.

fac-[Re(CO)(3)L](+) complexes with N-CH(2)-CH(2)-X-CH(2)-CH(2)-N tridentate ligands. synthetic, X-ray crystallographic, and NMR spectroscopic investigations.

PubMed

Christoforou, Anna Maria; Marzilli, Patricia A; Fronczek, Frank R; Marzilli, Luigi G

2007-12-24

Polyamine ligands (L) have excellent binding characteristics for the formation of fac-99mTc(CO)3-based radiopharmaceuticals. Normally, these L are elaborated so as to leave pendant groups designed to impart useful biodistribution characteristics to the fac-[99mTc(CO)3L] imaging agent. Our goal is to lay a foundation for understanding the features of the bound elaborated ligands by using the fac-[Re(CO)3L]-analogue approach with the minimal prototypical ligands, diethylenetriamine (dien) or simple dien-related derivatives. Treatment of the fac-[Re(CO)3(H2O)3]+ cation with such triamine (NNN) ligands afforded fac-[Re(CO)3L]+ complexes. Ligand variations included having a central amine thioether donor, thus allowing X-ray crystallographic and NMR spectroscopic comparisons of fac-[Re(CO)3L]+ complexes with NNN and NSN ligands. fac-[Re(CO)3L]+ complexes with two terminal exo-NH groups exhibit unusually far upfield exo-NH NMR signals in DMSO-d6. Upon the addition of Cl-, these exo-NH signals move downfield, while the signals of any endo-NH or central NH groups move very little. This behavior is attributed to the formation of 1:1 ion pairs having selective Cl- hydrogen bonding to both exo-NH groups. Base addition to a DMSO-d6 solution of meso-exo-[Re(CO)3(N,N',N''-Me3dien)]PF6 led to isomerization of only one NHMe group, producing the chiral isomer. The meso isomer did not form. The [Re(CO)3(N,N,N',N'',N''-pentamethyldiethylenetriamine)]triflate.[Re(CO)3(mu3-OH)]4.3.35H2O crystal, the first structure with a fac-[Re(CO)3L] complex cocrystallized with this well-known cluster, provided parameters for a bulky NNN ligand and also reveals CO-CO interlocking intermolecular interactions that could stabilize the crystal.

Right Ventricular Function in Preterm and Term Neonates: Reference Values for Right Ventricle Areas and Fractional Area of Change

PubMed Central

Levy, Philip T.; Diodena, Brittney; Holland, Mark R.; Sekarski, Timothy J.; Lee, Caroline K.; Mathur, Amit; Cade, W. Todd; Cahill, Alison G.; Hamvas, Aaron; Singh, Gautam K.

2015-01-01

Background Right Ventricle fractional area of change (RV FAC) is a quantitative two- dimensional echocardiographic measurement of RV function. RV FAC expresses the percentage change in the RV chamber area between end-diastole (RVEDA) to end-systole (RVESA). The objectives of this study were to determine the maturational (age- and weight- related) changes of RV FAC and RV areas and to establish reference values in healthy preterm and term neonates. Methods A prospective longitudinal study was conducted in 115 preterm infants (23-28 weeks gestational age at birth, 500-1500 gram). RV FAC was measured at 24 hours of age, 72 hours of age, 32 weeks and 36 weeks postmenstrual age (PMA). The maturational patterns of RVEDA, RVESA, and RV FAC were compared to 60 healthy full term infants in a cross sectional study (> 37 weeks, 3.5 +/− 1 kg), who received echocardiograms at birth (n=25) and one month of age (n=35). RVEDA and RVESA were traced in the RV focused apical 4-chamber view, and FAC was calculated using the formula: 100 * [(RVEDA – RVESA)/RVEDA)]. Premature infants that developed chronic lung disease or had a clinically and hemodynamically significant PDA were excluded (n=55) from the reference values. Intra- and inter- observer reproducibility analysis was performed. Results RV FAC ranged from 26% at birth to 35% by 36 weeks PMA in preterm infants (n=60) and increased almost two times faster in the first month of age as compared to healthy term infants (n=60). Similarly, RVEDA and RVESA increased throughout maturation in both term and preterm infants. RV FAC and RV areas correlated with weight (r=0.81, p<0.001), but were independent of gestational age at birth (r=0.3, p=0.45). RVEDA and RVESA correlated with PMA in weeks (r=0.81, p<0.001). RV FAC trended lower in preterm infants with bronchopulmonary dysplasia (p=0.04), but did not correlate to size of PDA (p=0.56). There was no difference in RV FAC based on gender or need for mechanical ventilation. Conclusions This study establishes reference values of RV areas (RVEDA and RVESA) and RV fractional area of change (RV FAC) in healthy term and preterm infants and tracks their maturational changes during postnatal development. These measures increase from birth to 36 weeks PMA, and this is reflective of the postnatal cardiac growth as a contributor to the maturation of cardiac function These measures are also linearly associated with increasing weight throughout maturation. This study suggests that two-dimensional RV FAC can be used as a complementary modality to assess global RV systolic function in neonates and facilitates its incorporation into clinical pediatric and neonatal guidelines. PMID:25753503

Generation of field-aligned current (FAC) and convection through the formation of pressure regimes: Correction for the concept of Dungey's convection

NASA Astrophysics Data System (ADS)

Tanaka, T.; Watanabe, M.; Den, M.; Fujita, S.; Ebihara, Y.; Kikuchi, T.; Hashimoto, K. K.; Kataoka, R.

2016-09-01

In this paper, we try to elucidate the generation mechanism of the field-aligned current (FAC) and coexisting convection. From the comparison between the theoretical prediction and the state of numerical solution from the high-resolution global simulation, we obtain the following conclusions about the distribution of dynamo, the magnetic field structure along the flow path that diverges Poynting flux, and energy conversion promoting the generation of electromagnetic energy. The dynamo for the region 1 FAC, which is in the high-latitude-side cusp-mantle region, has a structure in which magnetic field is compressed along the convection path by the slow mode motion. The dynamo for the region 2 FAC is in the ring current region at the inner edge of the plasma sheet, and has a structure in which magnetic field is curved outward along the convection path. Under these structures, electromagnetic energy is generated from the work done by pressure gradient force, in both dynamos for the region 1 and region 2 FACs. In these generation processes of the FACs, the excitation of convection and the formation of pressure regimes occur as interdependent processes. This structure leads to a modification in the way of understanding the Dungey's convection. Generation of the FAC through the formation of pressure regimes is essential even for the case of substorm onset.

Synthesis and characterization of fac-Re(CO)3-aspartic-N-monoacetic acid, a structural analogue of a potential new renal tracer, fac-99mTc(CO)3(ASMA).

PubMed

Klenc, Jeffrey; Lipowska, Malgorzata; Taylor, Andrew T; Marzilli, Luigi G

2012-09-01

The reaction of an aminopolycarboxylate ligand, as partic- N - m onoacetic a cid (ASMA), with [Re(CO) 3 (H 2 O) 3 ] + was examined. The tridentate coordination of ASMA to this Re I tricarbonyl precursor yielded fac -Re(CO) 3 (ASMA) as a mixture of diastereomers. The chemistry is analogous to that of the Tc I tricarbonyl complex, which yields fac - 99m Tc(CO) 3 (ASMA) under similar conditions. The formation, structure, and isomerization of fac -Re(CO) 3 (ASMA) products were characterized by HPLC, 1 H NMR spectroscopy, and X-ray crystallography. The two major fac -Re(CO) 3 (ASMA) diastereomeric products each have a linear ONO coordination mode with two adjacent five-membered chelate rings, but they differ in the endo or exo orientation of the uncoordinated acetate group, in agreement with expectations based on previous studies. Conditions have been identified for the expedient isomerization of fac -Re(CO) 3 (ASMA) to a mixture consisting primarily of one major product. Because different isomeric species typically have different pharmacokinetic characteristics, these conditions may provide for the practical isolation of a single 99m Tc(CO) 3 (ASMA) species, thus allowing the isolation of the isomer that has optimal imaging and pharmacokinetic characteristics. This information will aid in the design of future 99m Tc radiopharmaceuticals.

Synthesis and characterization of fac-Re(CO)3-aspartic-N-monoacetic acid, a structural analogue of a potential new renal tracer, fac-99mTc(CO)3(ASMA)

PubMed Central

Klenc, Jeffrey; Lipowska, Malgorzata; Taylor, Andrew T.; Marzilli, Luigi G.

2013-01-01

The reaction of an aminopolycarboxylate ligand, aspartic-N-monoacetic acid (ASMA), with [Re(CO)3(H2O)3]+ was examined. The tridentate coordination of ASMA to this ReI tricarbonyl precursor yielded fac-Re(CO)3(ASMA) as a mixture of diastereomers. The chemistry is analogous to that of the TcI tricarbonyl complex, which yields fac-99mTc(CO)3(ASMA) under similar conditions. The formation, structure, and isomerization of fac-Re(CO)3(ASMA) products were characterized by HPLC, 1H NMR spectroscopy, and X-ray crystallography. The two major fac-Re(CO)3(ASMA) diastereomeric products each have a linear ONO coordination mode with two adjacent five-membered chelate rings, but they differ in the endo or exo orientation of the uncoordinated acetate group, in agreement with expectations based on previous studies. Conditions have been identified for the expedient isomerization of fac-Re(CO)3(ASMA) to a mixture consisting primarily of one major product. Because different isomeric species typically have different pharmacokinetic characteristics, these conditions may provide for the practical isolation of a single 99mTc(CO)3(ASMA) species, thus allowing the isolation of the isomer that has optimal imaging and pharmacokinetic characteristics. This information will aid in the design of future 99mTc radiopharmaceuticals. PMID:24273448

48 CFR 301.605 - Contracting Officer designation of Contracting Officer Technical Representative.

Code of Federal Regulations, 2014 CFR

2014-10-01

... under HHS' FAC-COTR program before delegating authority to that individual to act as a COTR. Even if an individual is FAC-COTR-certified, a candidate becomes a COTR only when a Contracting Officer provides in... order file the individual's active FAC-COTR certificate. In the event that the HCA has granted an...

48 CFR 301.605 - Contracting Officer designation of Contracting Officer Technical Representative.

Code of Federal Regulations, 2013 CFR

2013-10-01

... under HHS' FAC-COTR program before delegating authority to that individual to act as a COTR. Even if an individual is FAC-COTR-certified, a candidate becomes a COTR only when a Contracting Officer provides in... order file the individual's active FAC-COTR certificate. In the event that the HCA has granted an...

75 FR 14067 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-40; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-23

... of the rule appearing in Federal Acquisition Circular (FAC) 2005-40 which amends the Federal... referring to FAC 2005-40 which precedes this document. These documents are also available via the Internet... the table below. Please cite FAC 2005-40 and the specific FAR case number. For information pertaining...

77 FR 23371 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-58; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-18

... Federal Acquisition Circular (FAC) 2005-58, which amends the Federal Acquisition Regulation (FAR). An... parties may obtain further information regarding this rule by referring to FAC 2005-58, which precedes... analyst whose name appears in the table below. Please cite FAC 2005-58 and the FAR case number. For...

48 CFR 301.605 - Contracting Officer designation of Contracting Officer Technical Representative.

Code of Federal Regulations, 2010 CFR

2010-10-01

... under HHS' FAC-COTR program before delegating authority to that individual to act as a COTR. Even if an individual is FAC-COTR-certified, a candidate becomes a COTR only when a Contracting Officer provides in... order file the individual's active FAC-COTR certificate. In the event that the HCA has granted an...

78 FR 13769 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-66; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-28

... Federal Acquisition Circular (FAC) 2005-66, which amends the Federal Acquisition Regulation (FAR). An... parties may obtain further information regarding this rule by referring to FAC 2005-66, which precedes... analyst whose name appears in the table below. Please cite FAC 2005-66 and the FAR case number. For...

48 CFR 301.605 - Contracting Officer designation of Contracting Officer Technical Representative.

Code of Federal Regulations, 2011 CFR

2011-10-01

... under HHS' FAC-COTR program before delegating authority to that individual to act as a COTR. Even if an individual is FAC-COTR-certified, a candidate becomes a COTR only when a Contracting Officer provides in... order file the individual's active FAC-COTR certificate. In the event that the HCA has granted an...

48 CFR 301.605 - Contracting Officer designation of Contracting Officer Technical Representative.

Code of Federal Regulations, 2012 CFR

2012-10-01

... under HHS' FAC-COTR program before delegating authority to that individual to act as a COTR. Even if an individual is FAC-COTR-certified, a candidate becomes a COTR only when a Contracting Officer provides in... order file the individual's active FAC-COTR certificate. In the event that the HCA has granted an...

A comparative analysis of single cell and droplet-based FACS for improving production phenotypes: Riboflavin overproduction in Yarrowia lipolytica.

PubMed

Wagner, James M; Liu, Leqian; Yuan, Shuo-Fu; Venkataraman, Maya V; Abate, Adam R; Alper, Hal S

2018-04-23

Evolutionary approaches to strain engineering inherently require the identification of suitable selection techniques for the product and phenotype of interest. In this work, we undertake a comparative analysis of two related but functionally distinct methods of high-throughput screening: traditional single cell fluorescence activated cell sorting (single cell FACS) and microdroplet-enabled FACS (droplet FACS) using water/oil/water (w/o/w) emulsions. To do so, we first engineer and evolve the non-conventional yeast Yarrowia lipolytica for high extracellular production of riboflavin (vitamin B2), an innately fluorescent product. Following mutagenesis and adaptive evolution, a direct parity-matched comparison of these two selection strategies was conducted. Both single cell FACS and droplet FACS led to significant increases in total riboflavin titer (32 and 54 fold relative to the parental PO1f strain, respectively). However, single cell FACS favored intracellular riboflavin accumulation (with only 70% of total riboflavin secreted) compared with droplet FACS that favored extracellular product accumulation (with 90% of total riboflavin secreted). We find that for the test case of riboflavin, the extent of secretion and total production were highly correlated. The resulting differences in production modes and levels clearly demonstrate the significant impact that selection approaches can exert on final evolutionary outcomes in strain engineering. Moreover, we note that these results provide a cautionary tale when intracellular read-outs of product concentration (including signals from biosensors) are used as surrogates for total production of potentially secreted products. In this regard, these results demonstrate that extracellular production is best assayed through an encapsulation technique when performing high throughput screening. Copyright © 2018 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Comparison of tricuspid annular plane systolic excursion with fractional area change for the evaluation of right ventricular systolic function: a meta-analysis

PubMed Central

Low, See-Wei; Pasha, Ahmed K; Howe, Carol L; Lee, Kwan S; Suryanarayana, Prakash G

2018-01-01

Background Accurate determination of right ventricular ejection fraction (RVEF) is challenging because of the unique geometry of the right ventricle. Tricuspidannular plane systolic excursion (TAPSE) and fractional area change (FAC) are commonly used echocardiographic quantitative estimates of RV function. Cardiac MRI (CMRI) has emerged as the gold standard for assessment of RVEF. We sought to summarise the available data on correlation of TAPSE and FAC with CMRI-derived RVEF and to compare their accuracy. Methods We searched PubMed, EMBASE, Web of Science, CINAHL, ClinicalTrials.gov and the Cochrane Library databases for studies that assessed the correlation of TAPSE or FAC with CMRI-derived RVEF. Data from each study selected were pooled and analysed to compare the correlation coefficient of TAPSE and FAC with CMRI-derived RVEF. Subgroup analysis was performed on patients with pulmonary hypertension. Results Analysis of data from 17 studies with a total of 1280 patients revealed that FAC had a higher correlation with CMRI-derived RVEF compared with TAPSE (0.56vs0.40, P=0.018). In patients with pulmonary hypertension, there was no statistical difference in the mean correlation coefficient of FAC and TAPSE to CMR (0.57vs0.46, P=0.16). Conclusions FAC provides a more accurate estimate of RV systolic function (RVSF) compared with TAPSE. Adoption of FAC as a routine tool for the assessment of RVSF should be considered, especially since it is also an independent predictor of morbidity and mortality. Further studies will be needed to compare other methods of echocardiographic measurement of RV function. PMID:29387425

Quantitative Evaluation of the Fetal Right and Left Ventricular Fractional Area Change Using Speckle Tracking Technology.

PubMed

DeVore, Greggory R; Klas, Berthold; Satou, Gary; Sklansky, Mark

2018-03-14

The purpose of this study was to measure the fractional area change (FAC) of the right and left ventricles in normal fetal hearts between 20 and 40 weeks of gestation using speckle-tracking software. The 4-chamber view of the fetal heart was obtained in 200 control fetuses between 20 and 40 weeks of gestation. The FAC was computed from the ventricular areas [((end-diastolic area) - (end-systolic area)/(end-diastolic area)) x 100] for the right and left ventricles and regressed against 7 independent biometric and age variables. The FAC was correlated with longitudinal fractional shortening (LFS) [((end-diastolic longitudinal length) - (end-systolic longitudinal length) /(end-diastolic longitudinal length)) x 100] obtained from the mid ventricular basal-apical lengths of the right and left ventricular chambers and the transverse fractional shortening (TFS) [((end-diastolic transverse length) - (end-systolic transverse length)/(end-diastolic transverse length)) x 100] from three transverse positions (base, mid, apical) located within each ventricular chamber. To evaluate potential clinical utility, the FAC, LFS, and TFS results were examined in 9 fetuses with congenital heart defects (CHD). Regression analysis demonstrated significant associations between the FAC and the biometric and age independent variables (R 2 = 0.13 - 0.15). The FAC was significantly correlated with the LFS (R 2 =0.18 to 0.28) and TFS (R 2 = 0.13 to 0.33). The 9 fetuses with CHD illustrated the interrelationship between the FAC, LFS, and TFS when identifying abnormal ventricular function. This study reports results from measuring the FAC of the right and left ventricles, and demonstrates a correlation with longitudinal fractional shortening (LFS) and transverse fractional shortening (TFS). This article is protected by copyright. All rights reserved.

Design of the free-air ionization chamber, FAC-IR-150, for X-ray dosimetry

NASA Astrophysics Data System (ADS)

Mohammadi, Seyed Mostafa; Tavakoli-Anbaran, Hossein

2018-03-01

The primary standard for X-ray dosimetry is based on the free-air ionization chamber (FAC). Therefore, the Atomic Energy Organization of Iran (AEOI) designed the free-air ionization chamber, FAC-IR-150, for low and medium energy X-ray dosimetry. The purpose of this work is the study of the free-air ionization chamber characteristics and the design of the FAC-IR-150. The FAC-IR-150 dosimeter has two parallel plates, a high voltage plate and a collector plate. A guard electrode surrounds the collector and is separated by an air gap. A group of guard strips is used between up and down electrodes to produce a uniform electric field in all the ion chamber volume. This design involves introducing the correction factors and determining the exact dimensions of the ionization chamber by using Monte Carlo simulation.

Free-air ionization chamber, FAC-IR-300, designed for medium energy X-ray dosimetry

NASA Astrophysics Data System (ADS)

Mohammadi, S. M.; Tavakoli-Anbaran, H.; Zeinali, H. Z.

2017-01-01

The primary standard for X-ray photons is based on parallel-plate free-air ionization chamber (FAC). Therefore, the Atomic Energy Organization of Iran (AEOI) is tried to design and build the free-air ionization chamber, FAC-IR-300, for low and medium energy X-ray dosimetry. The main aim of the present work is to investigate specification of the FAC-IR-300 ionization chamber and design it. FAC-IR-300 dosimeter is composed of two parallel plates, a high voltage (HV) plate and a collector plate, along with a guard electrode that surrounds the collector plate. The guard plate and the collector were separated by an air gap. For obtaining uniformity in the electric field distribution, a group of guard strips was used around the ionization chamber. These characterizations involve determining the exact dimensions of the ionization chamber by using Monte Carlo simulation and introducing correction factors.

TMSOTf assisted synthesis of 2'-deoxy-2'-[18F]fluoro-β-D-arabinofuranosylcytosine ([18F]FAC).

PubMed

Gangangari, Kishore K; Humm, John L; Larson, Steven M; Pillarsetty, Naga Vara Kishore

2018-01-01

[18F]FAC (2'-deoxy-2'-[18F]fluoro-β-D-arabinofuranosylcytosine, 1) is a versatile probe for imaging deoxycytidine kinase (dCK) expression levels in vivo. dCK is responsible for phosphorylation of deoxycytidine (dC, 2) and other nucleoside analogs, plays a key role in immune activation and has demonstrated to be one of the key enzymes in activating nucleoside based drugs including gemcitabine. Reported synthesis of [18F]FAC is high yielding but is quite challenging requiring bromination using HBr and careful drying of excess HBr which is critical for successful synthesis. Here in we report a simplified trimethylsilyl trifluoromethanesulfonate (TMSOTf) assisted synthesis of [18F]FAC eliminating the need of bromination and drying. [18F]FAC (β-anomer) was synthesized with average isolated decay corrected yield of 10.59 + 4.2% (n = 6) with radiochemical purity of >98% and total synthesis time of 158 + 19 min.

Effect of HNT on the Microstructure, Thermal and Mechanical Properties of Al/FACS-HNT Composites Produced by GPI

NASA Astrophysics Data System (ADS)

Siewiorek, A.; Malczyk, P.; Sobczak, N.; Sobczak, J. J.; Czulak, A.; Kozera, R.; Gude, M.; Boczkowska, A.; Homa, M.

2016-08-01

To develop an optimised manufacturing method of fly ash-reinforced metal matrix composites, the preliminary tests were performed on the cenospheres selected from fly ash (FACS) with halloysite nanotubes (HNTs) addition. The preform made out of FACS with and without the addition of HNT (with 5 and 10 wt.%) has been infiltrated by the pure aluminium (Al) via adapted gas pressure infiltration process. This paper reveals the influence of HNT addition on the microstructure (analysis was done by computed tomography and scanning electron microscopy combined with energy-dispersive x-ray spectroscopy), thermal properties (thermal expansion coefficient, thermal conductivity and specific heat) and the mechanical properties (hardness and compression test) of manufactured composites. The analysis of structure-property relationships for Al/FACS-HNT composites produced shows that the addition of 5 wt.% of HNT to FACS preform contributes to receiving of the best mechanical and structural properties of investigated composites.

Comparison of Whole-Cell SELEX Methods for the Identification of Staphylococcus Aureus-Specific DNA Aptamers

PubMed Central

Moon, Jihea; Kim, Giyoung; Park, Saet Byeol; Lim, Jongguk; Mo, Changyeun

2015-01-01

Whole-cell Systemic Evolution of Ligands by Exponential enrichment (SELEX) is the process by which aptamers specific to target cells are developed. Aptamers selected by whole-cell SELEX have high affinity and specificity for bacterial surface molecules and live bacterial targets. To identify DNA aptamers specific to Staphylococcus aureus, we applied our rapid whole-cell SELEX method to a single-stranded ssDNA library. To improve the specificity and selectivity of the aptamers, we designed, selected, and developed two categories of aptamers that were selected by two kinds of whole-cell SELEX, by mixing and combining FACS analysis and a counter-SELEX process. Using this approach, we have developed a biosensor system that employs a high affinity aptamer for detection of target bacteria. FAM-labeled aptamer sequences with high binding to S. aureus, as determined by fluorescence spectroscopic analysis, were identified, and aptamer A14, selected by the basic whole-cell SELEX using a once-off FACS analysis, and which had a high binding affinity and specificity, was chosen. The binding assay was evaluated using FACS analysis. Our study demonstrated the development of a set of whole-cell SELEX derived aptamers specific to S. aureus; this approach can be used in the identification of other bacteria. PMID:25884791

Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamers.

PubMed

Moon, Jihea; Kim, Giyoung; Park, Saet Byeol; Lim, Jongguk; Mo, Changyeun

2015-04-15

Whole-cell Systemic Evolution of Ligands by Exponential enrichment (SELEX) is the process by which aptamers specific to target cells are developed. Aptamers selected by whole-cell SELEX have high affinity and specificity for bacterial surface molecules and live bacterial targets. To identify DNA aptamers specific to Staphylococcus aureus, we applied our rapid whole-cell SELEX method to a single-stranded ssDNA library. To improve the specificity and selectivity of the aptamers, we designed, selected, and developed two categories of aptamers that were selected by two kinds of whole-cell SELEX, by mixing and combining FACS analysis and a counter-SELEX process. Using this approach, we have developed a biosensor system that employs a high affinity aptamer for detection of target bacteria. FAM-labeled aptamer sequences with high binding to S. aureus, as determined by fluorescence spectroscopic analysis, were identified, and aptamer A14, selected by the basic whole-cell SELEX using a once-off FACS analysis, and which had a high binding affinity and specificity, was chosen. The binding assay was evaluated using FACS analysis. Our study demonstrated the development of a set of whole-cell SELEX derived aptamers specific to S. aureus; this approach can be used in the identification of other bacteria.

Frontal affinity chromatography: A unique research tool for biospecific interaction that promotes glycobiology

PubMed Central

KASAI, Kenichi

2014-01-01

Combination of bioaffinity and chromatography gave birth to affinity chromatography. A further combination with frontal analysis resulted in creation of frontal affinity chromatography (FAC). This new versatile research tool enabled detailed analysis of weak interactions that play essential roles in living systems, especially those between complex saccharides and saccharide-binding proteins. FAC now becomes the best method for the investigation of saccharide-binding proteins (lectins) from viewpoints of sensitivity, accuracy, and efficiency, and is contributing greatly to the development of glycobiology. It opened a door leading to deeper understanding of the significance of saccharide recognition in life. The theory is also concisely described. PMID:25169774

The Extinction of Home Economics: A Study of Family and Consumer Sciences

ERIC Educational Resources Information Center

Antuna, Amber JoRie

2010-01-01

The purpose of this study was to determine the affects of the No Child Left Behind Act (NCLB) on Family and Consumer Science (FACS) program sustainment in the state of Arizona. FACS programs were not addressed in the NCLB mandates, but are part of the Arizona secondary education programming. FACS programs had seen a decrease in the number of…

Correlations between Geomagnetic Disturbances and Field-Aligned Currents during the 22-29 July 2004 Storm Time Interval

NASA Astrophysics Data System (ADS)

Hood, R.; Woodroffe, J. R.; Morley, S.; Aruliah, A. L.

2017-12-01

Using the CHAMP fluxgate magnetometer to calculate field-aligned current (FAC) densities and magnetic latitudes, with SuperMAG ground magnetometers analogously providing ground geomagnetic disturbances (GMD) magnetic perturbations and latitudes, we probe FAC locations and strengths as predictors of GMD locations and strengths. We also study the relationships between solar wind drivers and global magnetospheric activity, and both FACs and GMDs using IMF Bz and the Sym-H index. We present an event study of the 22-29 July 2004 storm time interval, which had particularly large GMDs given its storm intensity. We find no correlation between FAC and GMD magnitudes, perhaps due to CHAMP orbit limitations or ground magnetometer coverage. There is, however, a correlation between IMF Bz and nightside GMD magnitudes, supportive of their generation via tail reconnection. IMF Bz is also correlated with dayside FAC and GMD magnetic latitudes, indicating solar wind as an initial driver. The ring current influence increases during the final storm, with improved correlations between the Sym-H index and both FAC magnetic latitudes and GMD magnitudes. Sym-H index correlations may only be valid for higher intensity storms; a statistical analysis of many storms is needed to verify this.

Metal-assisted in situ formation of a tridentate acetylacetone ligand for complexation of fac-Re(CO)3+ for radiopharmaceutical applications.

PubMed

Benny, Paul D; Fugate, Glenn A; Barden, Adam O; Morley, Jennifer E; Silva-Lopez, Elsa; Twamley, Brendan

2008-04-07

Reaction of [NEt4]2[ReBr3(CO)3] with 2,4-pentanedione (acac) yields a complex of the type fac-Re(acac)(OH2)(CO)3 (1) under aqueous conditions. 1 was further reacted with a monodentate ligand (pyridine) to yield a fac-Re(acac)(pyridine)(CO)3 complex (2). Complex 1 was found to react with primary amines to generate a Schiff base (imine) in aqueous solutions. When a mixed-nitrogen donor bidentate ligand, 2-(2-aminoethyl)pyridine, that has different coordination affinities for fac-Re(acac)(OH2)(CO)3 was utilized, a unique tridentate ligand was formed in situ utilizing a metal-assisted Schiff base formation to yield a complex fac-Re(CO)3(3[(2-phenylethyl)imino]-2-pentanone) (3). Tridentate ligand formation was found to occur only with the Re-coordinated acac ligand. Reactions of acac with fac-Re(CO)3Br(2-(2-aminoethyl)pyridine) (4) or a mixture of [NEt4]2[ReBr3(CO)3], acac, and 2-(2-aminoethyl)pyridine did not yield the formation of complex 3 in water.

F-Area Acid/Caustic Basin groundwater monitoring report. First quarter 1995

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-06-01

During first quarter 1995, samples from the FAC monitoring wells at the F-Area Acid/Caustic Basin were collected and analyzed for herbicides/pesticides, indicator parameters, metals, nitrate, radionuclide indicators, volatile organic compounds, and other constituents. Piezometer FAC 5P and monitoring well FAC 6 were dry and could not be sampled. New monitoring wells FAC 9C, 10C, 11C, and 12C were completed in the Barnwell/McBean aquifer and were sampled for the first time during third quarter 1994 (first quarter 1995 is the third of four quarters of data required to support the closure of the basin). Analytical results that exceeded final Primary Drinkingmore » Water Standards (PDWS), other Savannah River Site (SRS) Flag 2 criteria, or the SRS turbidity standard of 50 NTU during the quarter were as follows: gross alpha exceeded the final PDWS and aluminum, iron, manganese, and total alpha-emitting radium exceeded the SRS Flag 2 criteria in one or more of the FAC wells. Turbidity exceeded the SRS standard (50 NTU) in wells FAC 3 and 11C. Groundwater flow direction and rate in the water table beneath the F-Area Acid/Caustic Basin were similar to past quarters.« less

Long-Term Prognostic Risk After Neoadjuvant Chemotherapy Associated With Residual Cancer Burden and Breast Cancer Subtype

PubMed Central

Wei, Caimiao; Gould, Rebekah; Yu, Xian; Zhang, Ya; Liu, Mei; Walls, Andrew; Bousamra, Alex; Ramineni, Maheshwari; Sinn, Bruno; Hunt, Kelly; Buchholz, Thomas A.; Valero, Vicente; Buzdar, Aman U.; Yang, Wei; Brewster, Abenaa M.; Moulder, Stacy; Pusztai, Lajos; Hatzis, Christos; Hortobagyi, Gabriel N.

2017-01-01

Purpose To determine the long-term prognosis in each phenotypic subset of breast cancer related to residual cancer burden (RCB) after neoadjuvant chemotherapy alone, or with concurrent human epidermal growth factor receptor 2 (HER2)–targeted treatment. Methods We conducted a pathologic review to measure the continuous RCB index (wherein pathologic complete response has RCB = 0; residual disease is categorized into three predefined classes of RCB index [RCB-I, RCB-II, and RCB-III]), and yp-stage of residual disease. Patients were prospectively observed for survival. Three patient cohorts received paclitaxel (T) followed by fluorouracil, doxorubicin, and cyclophosphamide (T/FAC): original development cohort (T/FAC-1), validation cohort (T/FAC-2), and independent validation cohort (T/FAC-3). Another validation cohort received FAC chemotherapy only, and a fifth cohort received concurrent trastuzumab (H) with sequential paclitaxel and fluorouracil, epirubicin, and cyclophosphamide (FEC; H+T/FEC). Phenotypic subsets were defined by hormone receptor (HR) and HER2 status at diagnosis, classified as HR-positive/HER2-negative, HER2-positive (HR-negative/HER2-positive or HR-positive/HER2-positive), or triple receptor–negative. Relapse-free survival estimates were determined from Kaplan-Meier analysis and compared using the log-rank test. Results Five cohorts (T/FAC-1 [n = 219], T/FAC-2 [n = 262], T/FAC-3 [n = 342], FAC [n = 132], and H+T/FEC [n = 203]) had median event-free follow-up of 13.5, 9.1, 6.8, 16.4, and 7.1 years, respectively. Continuous RCB index was prognostic within each phenotypic subset, independent of other clinical-pathologic variables. RCB classes stratified prognostic risk overall, within each phenotypic subset, and within yp-stage categories. Estimates of 10-year relapse-free survival rates in the four RCB classes (pathologic complete response, RCB-I, RCB-II, and RCB-III) were 86%, 81%, 55%, and 23% for triple receptor–negative; 83%, 97%, 74%, and 52% for HR-positive/HER2-negative in the combined T/FAC cohorts; and 95%, 77%, 47%, and 21% in the H+T/FEC cohort. Conclusion RCB was prognostic for long-term survival after neoadjuvant chemotherapy in all three phenotypic subsets of breast cancer. Our institutional findings should be externally validated. PMID:28135148

Activation requirements and responses to TLR ligands in human CD4+ T cells: comparison of two T cell isolation techniques.

PubMed

Lancioni, Christina L; Thomas, Jeremy J; Rojas, Roxana E

2009-05-15

Direct regulation of T cell function by microbial ligands through Toll-like receptors (TLR) is an emerging area of T cell biology. Currently either immunomagnetic cell sorting (IMACS) or fluorescence-activated cell sorting (FACS), are utilized to isolate T-cell subsets for such studies. However, it is unknown to what extent differences in T cell purity between these isolation techniques influence T cell functional assays. We compared the purity, response to mitogen, activation requirements, and response to TLR ligands between human CD4(+) T cells isolated either by IMACS (IMACS-CD4(+)) or by IMACS followed by FACS (IMACS/FACS-CD4(+)). As expected, IMACS-CD4(+) were less pure than IMACS/FACS-CD4(+) (92.5%+/-1.4% versus 99.7%+/-0.2%, respectively). Consequently, IMACS-CD4(+) proliferated and produced cytokines in response to mitogen alone and had lower activation requirements compared to IMACS/FACS-CD4(+). In addition IMACS-CD4(+) but not IMACS/FACS-CD4(+) responses were upregulated by the TLR-4 ligand lipopolysaccharide (LPS). On the other hand, TLR-2 and TLR-5 engagement induced costimulation in both IMACS-CD4(+) and highly purified IMACS-/FACS-CD4(+). Altogether these results indicate that small differences in cell purity can significantly alter T cell responses to TLR ligands. This study stresses the importance of a stringent purification method when investigating the role of microbial ligands in T cell function.

Optimizing acidified bleach solutions to improve sporicidal efficacy on building materials.

PubMed

Wood, J P; Calfee, M W; Clayton, M; Griffin-Gatchalian, N; Touati, A

2011-12-01

We evaluated whether lowering pH (with acetic acid) and raising free available chlorine (FAC) levels in bleach solutions would improve efficacy in inactivating Bacillus spores on different materials. We also determined how varying pH and FAC levels affected bleach stability. Acidified bleach solutions with pH levels of 4.5, 6 and 7.5 and FAC levels between 5000 and 10,000 ppm were evaluated for decontamination efficacy against Bacillus subtilis spores inoculated onto test coupons made from wood, ceramic and galvanized steel. Lowering the pH or increasing the FAC level improved efficacy in some of the tests, but depended on the material, which significantly affected decontamination efficacy. The acidified bleach at pH of 7.5 was significantly less effective than bleach at a pH of 4.5 or 6. The FAC levels in the bleach were the most stable at pH 4.5, and stability at pH 4.5 was not significantly affected by the initial FAC level. It may be advisable to use bleach solutions with lower pH (rather than high FAC levels) in light of both the decontamination efficacy and bleach stability results. For wood materials, use of sporicides other than acidified bleach may be warranted. These results may be useful in preparing acidified bleach solutions for decontamination of materials contaminated with spores such as Bacillus anthracis. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

The Perception and Mimicry of Facial Movements Predict Judgments of Smile Authenticity

PubMed Central

Korb, Sebastian; With, Stéphane; Niedenthal, Paula; Kaiser, Susanne; Grandjean, Didier

2014-01-01

The mechanisms through which people perceive different types of smiles and judge their authenticity remain unclear. Here, 19 different types of smiles were created based on the Facial Action Coding System (FACS), using highly controlled, dynamic avatar faces. Participants observed short videos of smiles while their facial mimicry was measured with electromyography (EMG) over four facial muscles. Smile authenticity was judged after each trial. Avatar attractiveness was judged once in response to each avatar’s neutral face. Results suggest that, in contrast to most earlier work using static pictures as stimuli, participants relied less on the Duchenne marker (the presence of crow’s feet wrinkles around the eyes) in their judgments of authenticity. Furthermore, mimicry of smiles occurred in the Zygomaticus Major, Orbicularis Oculi, and Corrugator muscles. Consistent with theories of embodied cognition, activity in these muscles predicted authenticity judgments, suggesting that facial mimicry influences the perception of smiles. However, no significant mediation effect of facial mimicry was found. Avatar attractiveness did not predict authenticity judgments or mimicry patterns. PMID:24918939

Developing an eBook-Integrated High-Fidelity Mobile App Prototype for Promoting Child Motor Skills and Taxonomically Assessing Children's Emotional Responses Using Face and Sound Topology.

PubMed

Brown, William; Liu, Connie; John, Rita Marie; Ford, Phoebe

2014-01-01

Developing gross and fine motor skills and expressing complex emotion is critical for child development. We introduce "StorySense", an eBook-integrated mobile app prototype that can sense face and sound topologies and identify movement and expression to promote children's motor skills and emotional developmental. Currently, most interactive eBooks on mobile devices only leverage "low-motor" interaction (i.e. tapping or swiping). Our app senses a greater breath of motion (e.g. clapping, snapping, and face tracking), and dynamically alters the storyline according to physical responses in ways that encourage the performance of predetermined motor skills ideal for a child's gross and fine motor development. In addition, our app can capture changes in facial topology, which can later be mapped using the Facial Action Coding System (FACS) for later interpretation of emotion. StorySense expands the human computer interaction vocabulary for mobile devices. Potential clinical applications include child development, physical therapy, and autism.

77 FR 1889 - Federal Acquisition Regulation; Set-Asides for Small Business; Extension of Comment Period

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-12

... ADMINISTRATION 48 CFR Parts 8, 12, 16, 19, 38, and 52 [FAC 2005-54; FAR Case 2011-024; Item VI; Docket 2011-0024... FAC 2005-54, FAR Case 2011- 024, by any of the following methods: Regulations.gov : http://www... comments only and cite FAC 2005-54, FAR Case 2011-024, in all correspondence related to this case. All...

Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments.

PubMed

Yamaoka, H; Nakayama-Imaohji, H; Horiuchi, I; Yamasaki, H; Nagao, T; Fujita, Y; Maeda, H; Goda, H; Kuwahara, T

2016-01-01

Chlorine is a principal disinfectant for food and environmental sanitation. Monitoring of free available chlorine (FAC) is essential for ensuring the efficacy of food disinfection processes that rely on chlorine. N,N-diethyl-p-phenylenediamine (DPD) is commonly used for FAC monitoring. However, here, we show that upon contact with bovine serum albumin (BSA) or broiler carcasses, chlorite (HClO2 )-based sanitizers acquire a pink colour, which can interfere with measurement of oxidized DPD absorbance at 513-550 nm. Alternatively, the pink colour did not interfere with 3,3',5,5'-tetramethylbenzidine (TMB)-based FAC monitoring. The FAC levels of NaClO and weakly acidified chlorous acid water (WACAW) were first adjusted by the TMB method and the killing activity of these sanitizers towards methicillin-resistant Staphylococcus aureus (MRSA) and feline calicivirus (FCV) was compared in the presence or absence of 0·5% BSA. At 200 ppm FAC, NaClO lost its bactericidal activity against MRSA after 10-min incubation with 0·5% BSA. Meanwhile, under the same conditions WACAW reduced the number of bacteria to below the detection limit. Similar results were obtained with FCV, indicating that the chlorite-based WACAW sanitizer is relatively stable under organic-matter-rich conditions. Moreover, TMB is suitable for in situ FAC monitoring of chlorite-based sanitizers in food and environmental disinfection processes. For practical applications of chlorine in food processing, monitoring of FAC is critical to validate disinfection efficacy. In this study we found that chlorite-based sanitizers acquired a pink colour upon contact with BSA or broiler carcasses. This pink colour interfered with FAC monitoring by methods that measure oxidized N,N-diethyl-p-phenylenediamine absorbance between 513-550 nm. Alternatively, FAC levels of chlorite-based sanitizers could be monitored using the absorbance of 3,3',5,5'-tetramethylbenzidine at 650 nm, which does not overlap with the acquired pink colour. These data provide valuable information for safety management of disinfection processes that use chlorite-based sanitizers. © 2015 The Society for Applied Microbiology.

Time-resolved EPR study on the photoexcited triplet state of the electron-donor-acceptor complex formed in the system of fac-tris[2-(4-octyl-phenyl) pyridine] iridium(III) and tetracene.

PubMed

Zhebin, Fu; Shuhei, Yoshioka; Hisao, Murai

2014-01-09

The physical properties of the phosphorescent organic light-emitting diode material fac-tris(phenylpyridine) iridium(III), Ir(ppy)3, have been reported with experimental and theoretical studies. Here, the photochemical properties of the excited triplet state of partially modified fac-tris[2-(4-octyl-phenyl) pyridine] iridium(III), Ir(C8ppy)3, were investigated using time-resolved electron paramagnetic resonance (tr-EPR) and optical methods by adding tetracene in the toluene solution. The tr-EPR observation at 77 K revealed the following two species: the excited triplet state of tetracene and another triplet species with zero field splitting parameters of |D| = 0.088 cm(-1) and |E| = 0.018 cm(-1) with characteristic spin polarization. The latter species was assigned to the electron-donor-acceptor (EDA) complex formed between Ir(C8ppy)3 and tetracene. The mechanism of formation and the properties of this EDA complex, including the information on the principal axes of (3)Ir(C8ppy)3*, are discussed.

Variability of Burkholderia pseudomallei Strain Sensitivities to Chlorine Disinfection▿

PubMed Central

O'Connell, Heather A.; Rose, Laura J.; Shams, Alicia; Bradley, Meranda; Arduino, Matthew J.; Rice, Eugene W.

2009-01-01

Burkholderia pseudomallei is a select agent and the causative agent of melioidosis. Variations in previously reported chlorine and monochloramine concentration time (Ct) values for disinfection of this organism make decisions regarding the appropriate levels of chlorine in water treatment systems difficult. This study identified the variation in Ct values for 2-, 3-, and 4-log10 reductions of eight environmental and clinical isolates of B. pseudomallei in phosphate-buffered water. The greatest calculated Ct values for a 4-log10 inactivation were 7.8 mg·min/liter for free available chlorine (FAC) at pH 8 and 5°C and 550 mg·min/liter for monochloramine at pH 8 and 5°C. Ionic strength of test solutions, culture hold times in water, and cell washing were ruled out as sources of the differences in prior observations. Tolerance to FAC was correlated with the relative amount of extracellular material produced by each isolate. Solid-phase cytometry analysis using an esterase-cleaved fluorochrome assay detected a 2-log10-higher level of organisms based upon metabolic activity than did culture, which in some cases increased Ct values by fivefold. Despite strain-to-strain variations in Ct values of 17-fold for FAC and 2.5-fold for monochloramine, standard FAC disinfection practices utilized in the United States should disinfect planktonic populations of these B. pseudomallei strains by 4 orders of magnitude in less than 10 min at the tested temperatures and pH levels. PMID:19542324

Linear response of field-aligned currents to the interplanetary electric field

NASA Astrophysics Data System (ADS)

Weimer, D. R.; Edwards, T. R.; Olsen, Nils

2017-08-01

Many studies that have shown that the ionospheric, polar cap electric potentials (PCEPs) exhibit a "saturation" behavior in response to the level of the driving by the solar wind. As the magnitudes of the interplanetary magnetic field (IMF) and electric field (IEF) increase, the PCEP response is linear at low driving levels, followed with a rollover to a more constant level. While there are several different theoretical explanations for this behavior, so far, no direct observational evidence has existed to confirm any particular model. In most models of this saturation, the interaction of the field-aligned currents (FACs) with the solar wind/magnetosphere/ionosphere system has a role. As the FACs are more difficult to measure, their behavior in response to the level of the IEF has not been investigated as thoroughly. In order to resolve the question of whether or not the FAC also exhibit saturation, we have processed the magnetic field measurements from the Ørsted, CHAMP, and Swarm missions, spanning more than a decade. As the amount of current in each region needs to be known, a new technique is used to separate and sum the current by region, widely known as R0, R1, and R2. These totals are found separately for the dawnside and duskside. Results indicate that the total FAC has a response to the IEF that is highly linear, continuing to increase well beyond the level at which the electric potentials saturate. The currents within each region have similar behavior.

Energy Levels and Radiative Rates for Transitions in F-like Sc XIII and Ne-like Sc XII and Y XXX

NASA Astrophysics Data System (ADS)

Aggarwal, Kanti

2018-05-01

Energy levels, radiative rates and lifetimes are reported for F-like Sc~XIII and Ne-like Sc~XII and Y~XXX for which the general-purpose relativistic atomic structure package ({\\sc grasp}) has been adopted. For all three ions limited data exist in the literature but comparisons have been made wherever possible to assess the accuracy of the calculations. In the present work the lowest 102, 125 and 139 levels have been considered for the respective ions. Additionally, calculations have also been performed with the flexible atomic code ({\\sc fac}) to (particularly) confirm the accuracy of energy levels.

X-ray spectroscopy of E2 and M3 transitions in Ni-like W

DOE Office of Scientific and Technical Information (OSTI.GOV)

Clementson, J.; Beiersdorfer, P.; Gu, M. F.

2010-01-15

The electric quadrupole (E2) and magnetic octupole (M3) ground-state transitions in Ni-like W{sup 46+} have been measured using high-resolution crystal spectroscopy at the LLNL electron-beam ion trap facility. The lines fall in the soft x-ray region near 7.93 A and were originally observed as an unresolved feature in tokamak plasmas. Using flat ammonium dihydrogen phosphate and quartz crystals, the wavelengths, intensities, and polarizations of the two lines have been measured for various electron-beam energies and compared to intensity and polarization calculations performed using the Flexible Atomic Code (FAC).

48 CFR 301.607-71 - FAC-P/PM levels and requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

.... (2) The FAI describes the following three sets of general core competencies on its Web site: (3..., systems engineering, test and evaluation, contracting, and business. (6) Specific core competencies also...

Process optimization for the preparation of oligomycin-loaded folate-conjugated chitosan nanoparticles as a tumor-targeted drug delivery system using a two-level factorial design method.

PubMed

Zu, Yuangang; Zhao, Qi; Zhao, Xiuhua; Zu, Shuchong; Meng, Li

2011-01-01

Oligomycin-A (Oli-A), an anticancer drug, was loaded to the folate (FA)-conjugated chitosan as a tumor-targeted drug delivery system for the purpose of overcoming the nonspecific targeting characteristics and the hydrophobicity of the compound. The two-level factorial design (2-LFD) was applied to modeling the preparation process, which was composed of five independent variables, namely FA-conjugated chitosan (FA-CS) concentration, Oli-A concentration, sodium tripolyphosphate (TPP) concentration, the mass ratio of FA-CS to TPP, and crosslinking time. The mean particle size (MPS) and the drug loading rate (DLR) of the resulting Oli-loaded FA-CS nanoparticles (FA-Oli-CSNPs) were used as response variables. The interactive effects of the five independent variables on the response variables were studied. The characteristics of the nanoparticles, such as amount of FA conjugation, drug entrapment rate (DER), DLR, surface morphology, and release kinetics properties in vitro were investigated. The FA-Oli-CSNPs with MPS of 182.6 nm, DER of 17.3%, DLR of 58.5%, and zeta potential (ZP) of 24.6 mV were obtained under optimum conditions. The amount of FA conjugation was 45.9 mg/g chitosan. The FA-Oli-CSNPs showed sustained-release characteristics for 576 hours in vitro. The results indicated that FA-Oli-CSNPs obtained as a targeted drug delivery system could be effective in the therapy of leukemia in the future.

A High-resolution Model of Field-aligned Currents Through Empirical Orthogonal Functions Analysis (MFACE)

NASA Technical Reports Server (NTRS)

He, Maosheng; Vogt, Joachim; Luehr, Hermann; Sorbalo, Eugen; Blagau, Adrian; Le, Guan; Lu, Gang

2012-01-01

Ten years of CHAMP magnetic field measurements are integrated into MFACE, a model of field-aligned currents (FACs) using empirical orthogonal functions (EOFs). EOF1 gives the basic Region-1/Region-2 pattern varying mainly with the interplanetary magnetic field Bz component. EOF2 captures separately the cusp current signature and By-related variability. Compared to existing models, MFACE yields significantly better spatial resolution, reproduces typically observed FAC thickness and intensity, improves on the magnetic local time (MLT) distribution, and gives the seasonal dependence of FAC latitudes and the NBZ current signature. MFACE further reveals systematic dependences on By, including 1) Region-1/Region-2 topology modifications around noon; 2) imbalance between upward and downward maximum current density; 3) MLT location of the Harang discontinuity. Furthermore, our procedure allows quantifying response times of FACs to solar wind driving at the bow shock nose: we obtain 20 minutes and 35-40 minutes lags for the FAC density and latitude, respectively.

The particle carriers of field-aligned currents in the Earth's magnetotail during a substorm

NASA Astrophysics Data System (ADS)

Cheng, Z. W.; Zhang, J. C.; Shi, J. K.; Kistler, L. M.; Dunlop, M.; Dandouras, I.; Fazakerley, A.

2016-04-01

Although the particle carriers of field-aligned currents (FACs) in the Earth's magnetotail play an important role in the transfer of momentum and energy between the solar wind, magnetosphere, and ionosphere, the characteristics of the FAC carriers have been poorly understood. Taking advantage of multiinstrument magnetic field and plasma data collected by the four spacecraft of the Cluster constellation as they traversed the northern plasma sheet boundary layer in the magnetotail on 14 September 2004, we identified the species type and energy range of the FAC carriers for the first time. The results indicate that part of tailward FACs is carried by energetic keV ions, which are probably originated from the ionosphere through outflow, and they are not too small (~2 nA/m2) to be ignored. The earthward (tailward) FACs are mainly carried by the dominant tailward (earthward) motion of electrons, and higher-energy electrons (from ~0.5 to 26 keV) are the main carriers.

Characterization of Intraocular Pressure Increases and Management Strategies Following Treatment With Fluocinolone Acetonide Intravitreal Implants in the FAME Trials.

PubMed

Parrish, Richard K; Campochiaro, Peter A; Pearson, P Andrew; Green, Ken; Traverso, Carlo E

2016-05-01

To compare elevated intraocular pressure (IOP) management and outcomes among patients with diabetic macular edema who received fluocinolone acetonide (FAc) implants versus sham-control treatment and explore the prior ocular steroid exposure impact on IOP outcomes. Best-corrected visual acuity (BCVA) was measured using Early Treatment Diabetic Retinopathy Study charts or electronic VA testers. Goldmann applanation tonometry was used to measure IOP. Elevated IOP was more common in FAc-versus sham control-treated patients. Medication, and less often trabeculoplasty or surgery, was used to lower IOP without affecting VA outcomes. No patient treated with 0.2 µg/day FAc who received prior ocular steroid required IOP-lowering surgery. Elevated IOP may occur following FAc implant receipt; however, in the present study, it was manageable and did not impact vision outcomes. Patients previously treated with ocular steroid did not require IOP-lowering surgery following 0.2 µg/day FAc implant administration. [Ophthalmic Surg Lasers Imaging Retina. 2016;47:426-435.]. Copyright 2016, SLACK Incorporated.

TMSOTf assisted synthesis of 2’-deoxy-2’-[18F]fluoro-β-D-arabinofuranosylcytosine ([18F]FAC)

PubMed Central

Humm, John L.; Larson, Steven M.; Pillarsetty, Naga Vara Kishore

2018-01-01

[18F]FAC (2’-deoxy-2’-[18F]fluoro-β-D-arabinofuranosylcytosine, 1) is a versatile probe for imaging deoxycytidine kinase (dCK) expression levels in vivo. dCK is responsible for phosphorylation of deoxycytidine (dC, 2) and other nucleoside analogs, plays a key role in immune activation and has demonstrated to be one of the key enzymes in activating nucleoside based drugs including gemcitabine. Reported synthesis of [18F]FAC is high yielding but is quite challenging requiring bromination using HBr and careful drying of excess HBr which is critical for successful synthesis. Here in we report a simplified trimethylsilyl trifluoromethanesulfonate (TMSOTf) assisted synthesis of [18F]FAC eliminating the need of bromination and drying. [18F]FAC (β-anomer) was synthesized with average isolated decay corrected yield of 10.59 + 4.2% (n = 6) with radiochemical purity of >98% and total synthesis time of 158 + 19 min. PMID:29715301

Water-quality trends for selected sites in the Boulder River and Tenmile Creek watersheds, Montana, based on data collected during water years 1997-2013

USGS Publications Warehouse

Sando, Steven K.; Clark, Melanie L.; Cleasby, Thomas E.; Barnhart, Elliott P.

2015-01-01

Trend results for sites in the Tenmile Creek watershed generally are more variable and difficult to interpret than for sites in the Boulder River watershed. Trend results for Tenmile Creek above City Diversion (site 11) and Minnehaha Creek near Rimini (site 12) for water years 2000–13 indicate decreasing trends in FACs of cadmium, copper, and zinc. The magnitudes of the decreasing trends in FACs of copper generally are moderate and statistically significant for sites 11 and 12. The magnitudes of the decreasing trends in FACs of cadmium and zinc for site 11 are minor to small and not statistically significant; however, the magnitudes for site 12 are moderate and statistically significant. In general, patterns in FACs for Tenmile Creek near Rimini (site 13) are not well represented by fitted trends within the short data collection period, which might indicate that the trend-analysis structure of the study is not appropriate for describing trends in FACs for site 13. The large decreasing trend in FACs of suspended sediment is the strongest indication of change in water quality during the short period of record for site 13; however, this trend is not statistically significant.

Cation-Dependent Light-Induced Halide Demixing in Hybrid Organic–Inorganic Perovskites

DOE PAGES

Sutter-Fella, Carolin M.; Ngo, Quynh P.; Cefarin, Nicola; ...

2018-04-30

Mixed cation metal halide perovskites with increased power conversion efficiency, negligible hysteresis, and improved long-term stability under illumination, moisture, and thermal stressing have emerged as promising compounds for photovoltaic and optoelectronic applications. In this paper, we shed light on photoinduced halide demixing using in situ photoluminescence spectroscopy and in situ synchrotron X-ray diffraction (XRD) to directly compare the evolution of composition and phase changes in CH(NH 2) 2CsPb-halide (FACsPb-) and CH 3NH 3Pb-halide (MAPb-) perovskites upon illumination, thereby providing insights into why FACs-perovskites are less prone to halide demixing than MA-perovskites. We find that halide demixing occurs in both materials.more » However, the I-rich domains formed during demixing accumulate strain in FACsPb-perovskites but readily relax in MA-perovskites. The accumulated strain energy is expected to act as a stabilizing force against halide demixing and may explain the higher Br composition threshold for demixing to occur in FACsPb-halides. In addition, we find that while halide demixing leads to a quenching of the high-energy photoluminescence emission from MA-perovskites, the emission is enhanced from FACs-perovskites. This behavior points to a reduction of nonradiative recombination centers in FACs-perovskites arising from the demixing process and buildup of strain. FACsPb-halide perovskites exhibit excellent intrinsic material properties with photoluminescence quantum yields that are comparable to MA-perovskites. Finally, because improved stability is achieved without sacrificing electronic properties, these compositions are better candidates for photovoltaic applications, especially as wide bandgap absorbers in tandem cells.« less

Cation-Dependent Light-Induced Halide Demixing in Hybrid Organic-Inorganic Perovskites.

PubMed

Sutter-Fella, Carolin M; Ngo, Quynh P; Cefarin, Nicola; Gardner, Kira L; Tamura, Nobumichi; Stan, Camelia V; Drisdell, Walter S; Javey, Ali; Toma, Francesca M; Sharp, Ian D

2018-06-13

Mixed cation metal halide perovskites with increased power conversion efficiency, negligible hysteresis, and improved long-term stability under illumination, moisture, and thermal stressing have emerged as promising compounds for photovoltaic and optoelectronic applications. Here, we shed light on photoinduced halide demixing using in situ photoluminescence spectroscopy and in situ synchrotron X-ray diffraction (XRD) to directly compare the evolution of composition and phase changes in CH(NH 2 ) 2 CsPb-halide (FACsPb-) and CH 3 NH 3 Pb-halide (MAPb-) perovskites upon illumination, thereby providing insights into why FACs-perovskites are less prone to halide demixing than MA-perovskites. We find that halide demixing occurs in both materials. However, the I-rich domains formed during demixing accumulate strain in FACsPb-perovskites but readily relax in MA-perovskites. The accumulated strain energy is expected to act as a stabilizing force against halide demixing and may explain the higher Br composition threshold for demixing to occur in FACsPb-halides. In addition, we find that while halide demixing leads to a quenching of the high-energy photoluminescence emission from MA-perovskites, the emission is enhanced from FACs-perovskites. This behavior points to a reduction of nonradiative recombination centers in FACs-perovskites arising from the demixing process and buildup of strain. FACsPb-halide perovskites exhibit excellent intrinsic material properties with photoluminescence quantum yields that are comparable to MA-perovskites. Because improved stability is achieved without sacrificing electronic properties, these compositions are better candidates for photovoltaic applications, especially as wide bandgap absorbers in tandem cells.

Long-term outcomes of phakic patients with diabetic macular oedema treated with intravitreal fluocinolone acetonide (FAc) implants.

PubMed

Yang, Y; Bailey, C; Holz, F G; Eter, N; Weber, M; Baker, C; Kiss, S; Menchini, U; Ruiz Moreno, J M; Dugel, P; Lotery, A

2015-09-01

Diabetic macular oedema (DMO) is a leading cause of blindness in working-age adults. Slow-release, nonbioerodible fluocinolone acetonide (FAc) implants have shown efficacy in the treatment of DMO; however, the National Institute for Health and Care Excellence recommends that FAc should be used in patients with chronic DMO considered insufficiently responsive to other available therapies only if the eye to be treated is pseudophakic. The goal of this analysis was to examine treatment outcomes in phakic patients who received 0.2 μg/day FAc implant. This analysis of the phase 3 FAME (Fluocinolone Acetonide in Diabetic Macular Edema) data examines the safety and efficacy of FAc implants in patients who underwent cataract extraction before (cataract before implant (CBI) group) or after (cataract after implant (CAI) group) receiving the implant. The data were further examined by DMO duration. Best corrected visual acuity (BCVA) after 36 months was comparable in the CAI and CBI groups. Both the percentage of patients gaining ≥ 3 lines of vision and mean change in BCVA letter score were numerically greater in the CAI group. In addition, most patients who underwent cataract surgery experienced a net gain in BCVA from presurgery baseline as well as from original study baseline. These data support the use of 0.2 μg/day FAc implants in phakic as well as in pseudophakic patients. These findings will serve as a pilot for design of future studies to evaluate the potential protective effect of FAc implants before cataract surgery in patients with DMO and cataract.

Cation-Dependent Light-Induced Halide Demixing in Hybrid Organic–Inorganic Perovskites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sutter-Fella, Carolin M.; Ngo, Quynh P.; Cefarin, Nicola

Mixed cation metal halide perovskites with increased power conversion efficiency, negligible hysteresis, and improved long-term stability under illumination, moisture, and thermal stressing have emerged as promising compounds for photovoltaic and optoelectronic applications. In this paper, we shed light on photoinduced halide demixing using in situ photoluminescence spectroscopy and in situ synchrotron X-ray diffraction (XRD) to directly compare the evolution of composition and phase changes in CH(NH 2) 2CsPb-halide (FACsPb-) and CH 3NH 3Pb-halide (MAPb-) perovskites upon illumination, thereby providing insights into why FACs-perovskites are less prone to halide demixing than MA-perovskites. We find that halide demixing occurs in both materials.more » However, the I-rich domains formed during demixing accumulate strain in FACsPb-perovskites but readily relax in MA-perovskites. The accumulated strain energy is expected to act as a stabilizing force against halide demixing and may explain the higher Br composition threshold for demixing to occur in FACsPb-halides. In addition, we find that while halide demixing leads to a quenching of the high-energy photoluminescence emission from MA-perovskites, the emission is enhanced from FACs-perovskites. This behavior points to a reduction of nonradiative recombination centers in FACs-perovskites arising from the demixing process and buildup of strain. FACsPb-halide perovskites exhibit excellent intrinsic material properties with photoluminescence quantum yields that are comparable to MA-perovskites. Finally, because improved stability is achieved without sacrificing electronic properties, these compositions are better candidates for photovoltaic applications, especially as wide bandgap absorbers in tandem cells.« less

Spectroscopic Characterization of Aqua [ fac-Tc(CO)3]+ Complexes at High Ionic Strength.

PubMed

Chatterjee, Sayandev; Hall, Gabriel B; Engelhard, Mark H; Du, Yingge; Washton, Nancy M; Lukens, Wayne W; Lee, Sungsik; Pearce, Carolyn I; Levitskaia, Tatiana G

2018-06-18

Understanding fundamental Tc chemistry is important to both the remediation of nuclear waste and the reprocessing of nuclear fuel; however, current knowledge of the electronic structure and spectral signatures of low-valent Tc compounds significantly lags behind the remainder of the d-block elements. In particular, identification and treatment of Tc speciation in legacy nuclear waste is challenging due to the lack of reference data especially for Tc compounds in the less common oxidation states (I-VI). In an effort to establish a spectroscopic library corresponding to the relevant conditions of extremely high ionic strength typical for the legacy nuclear waste, compounds with the general formula of [ fac-Tc(CO) 3 (OH 2 ) 3- n (OH) n ] 1- n (where n = 0-3) were examined by a range of spectroscopic techniques including 99 Tc/ 13 C NMR, IR, XPS, and XAS. In the series of monomeric aqua species, stepwise hydrolysis results in the increase of the Tc metal center electron density and corresponding progressive decrease of the Tc-C bond distances, Tc electron binding energies, and carbonyl stretching frequencies in the order [ fac-Tc(CO) 3 (OH 2 ) 3 ] + > [ fac-Tc(CO) 3 (OH 2 ) 2 (OH)] > [ fac-Tc(CO) 3 (OH 2 )(OH) 2 ] - . These results correlate with established trends of the 99 Tc upfield chemical shift and carbonyl 13 C downfield chemical shift. The lone exception is [ fac-Tc(CO) 3 (OH)] 4 which exhibits a comparatively low electron density at the metal center attributed to the μ 3 -bridging nature of the - OH ligands causing less σ-donation and no π-donation. This work also reports the first observations of these compounds by XPS and [ fac-Tc(CO) 3 Cl 3 ] 2- by XAS. The unique and distinguishable spectral features of the aqua [ fac-Tc(CO) 3 ] + complexes lay the foundation for their identification in the complex aqueous matrixes.

Plant volatile eliciting FACs in lepidopteran caterpillars, fruit flies, and crickets: a convergent evolution or phylogenetic inheritance?

PubMed Central

Yoshinaga, Naoko; Abe, Hiroaki; Morita, Sayo; Yoshida, Tetsuya; Aboshi, Takako; Fukui, Masao; Tumlinson, James H.; Mori, Naoki

2013-01-01

Fatty acid amino acid conjugates (FACs), first identified in lepidopteran caterpillar spit as elicitors of plant volatile emission, also have been reported as major components in gut tracts of Drosophila melanogaster and cricket Teleogryllus taiwanemma. The profile of FAC analogs in these two insects was similar to that of tobacco hornworm Manduca sexta, showing glutamic acid conjugates predominantly over glutamine conjugates. The physiological function of FACs is presumably to enhance nitrogen assimilation in Spodoptera litura larvae, but in other insects it is totally unknown. Whether these insects share a common synthetic mechanism of FACs is also unclear. In this study, the biosynthesis of FACs was examined in vitro in five lepidopteran species (M. sexta, Cephonodes hylas, silkworm, S. litura, and Mythimna separata), fruit fly larvae and T. taiwanemma. The fresh midgut tissues of all of the tested insects showed the ability to synthesize glutamine conjugates in vitro when incubated with glutamine and sodium linolenate. Such direct conjugation was also observed for glutamic acid conjugates in all the insects but the product amount was very small and did not reflect the in vivo FAC patterns in each species. In fruit fly larvae, the predominance of glutamic acid conjugates could be explained by a shortage of substrate glutamine in midgut tissues, and in M. sexta, a rapid hydrolysis of glutamine conjugates has been reported. In crickets, we found an additional unique biosynthetic pathway for glutamic acid conjugates. T. taiwanemma converted glutamine conjugates to glutamic acid conjugates by deaminating the side chain of the glutamine moiety. Considering these findings together with previous results, a possibility that FACs in these insects are results of convergent evolution cannot be ruled out, but it is more likely that the ancestral insects had the glutamine conjugates and crickets and other insects developed glutamic acid conjugates in a different way. PMID:24744735

SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC): identification of early mediators of insect responses

PubMed Central

2010-01-01

Background Plants trigger and tailor defense responses after perception of the oral secretions (OS) of attacking specialist lepidopteran larvae. Fatty acid-amino acid conjugates (FACs) in the OS of the Manduca sexta larvae are necessary and sufficient to elicit the herbivory-specific responses in Nicotiana attenuata, an annual wild tobacco species. How FACs are perceived and activate signal transduction mechanisms is unknown. Results We used SuperSAGE combined with 454 sequencing to quantify the early transcriptional changes elicited by the FAC N-linolenoyl-glutamic acid (18:3-Glu) and virus induced gene silencing (VIGS) to examine the function of candidate genes in the M. sexta-N. attenuata interaction. The analysis targeted mRNAs encoding regulatory components: rare transcripts with very rapid FAC-elicited kinetics (increases within 60 and declines within 120 min). From 12,744 unique Tag sequences identified (UniTags), 430 and 117 were significantly up- and down-regulated ≥ 2.5-fold, respectively, after 18:3-Glu elicitation compared to wounding. Based on gene ontology classification, more than 25% of the annotated UniTags corresponded to putative regulatory components, including 30 transcriptional regulators and 22 protein kinases. Quantitative PCR analysis was used to analyze the FAC-dependent regulation of a subset of 27 of these UniTags and for most of them a rapid and transient induction was confirmed. Six FAC-regulated genes were functionally characterized by VIGS and two, a putative lipid phosphate phosphatase (LPP) and a protein of unknown function, were identified as important mediators of the M. sexta-N. attenuata interaction. Conclusions The analysis of the early changes in the transcriptome of N. attenuata after FAC elicitation using SuperSAGE/454 has identified regulatory genes involved in insect-specific mediated responses in plants. Moreover, it has provided a foundation for the identification of additional novel regulators associated with this process. PMID:20398280

SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC): identification of early mediators of insect responses.

PubMed

Gilardoni, Paola A; Schuck, Stefan; Jüngling, Ruth; Rotter, Björn; Baldwin, Ian T; Bonaventure, Gustavo

2010-04-14

Plants trigger and tailor defense responses after perception of the oral secretions (OS) of attacking specialist lepidopteran larvae. Fatty acid-amino acid conjugates (FACs) in the OS of the Manduca sexta larvae are necessary and sufficient to elicit the herbivory-specific responses in Nicotiana attenuata, an annual wild tobacco species. How FACs are perceived and activate signal transduction mechanisms is unknown. We used SuperSAGE combined with 454 sequencing to quantify the early transcriptional changes elicited by the FAC N-linolenoyl-glutamic acid (18:3-Glu) and virus induced gene silencing (VIGS) to examine the function of candidate genes in the M. sexta-N. attenuata interaction. The analysis targeted mRNAs encoding regulatory components: rare transcripts with very rapid FAC-elicited kinetics (increases within 60 and declines within 120 min). From 12,744 unique Tag sequences identified (UniTags), 430 and 117 were significantly up- and down-regulated >or= 2.5-fold, respectively, after 18:3-Glu elicitation compared to wounding. Based on gene ontology classification, more than 25% of the annotated UniTags corresponded to putative regulatory components, including 30 transcriptional regulators and 22 protein kinases. Quantitative PCR analysis was used to analyze the FAC-dependent regulation of a subset of 27 of these UniTags and for most of them a rapid and transient induction was confirmed. Six FAC-regulated genes were functionally characterized by VIGS and two, a putative lipid phosphate phosphatase (LPP) and a protein of unknown function, were identified as important mediators of the M. sexta-N. attenuata interaction. The analysis of the early changes in the transcriptome of N. attenuata after FAC elicitation using SuperSAGE/454 has identified regulatory genes involved in insect-specific mediated responses in plants. Moreover, it has provided a foundation for the identification of additional novel regulators associated with this process.

Activation of the manganese(I) tricarbonyl core by selective variation of bidentate ligands (L,L'-Bid = N,N' and N,O donor atom sets) in fac-[Mn(CO)3(L,L'-Bid)(CH3OH)](n) complexes.

PubMed

Twala, T N; Schutte-Smith, M; Roodt, A; Visser, H G

2015-02-21

A range of fac-[Mn(CO)3(L,L'-Bid)(H2O)](n) (L,L'-Bid = neutral or monoanionic bidentate ligands with varied L,L' donor atoms, N,N' and N,O, 1,10-phenanthroline, 2,2'-bipyridine, 2-picolinate, 2,4-quinolinate; n = 0, +1) has been synthesized and the methanol substitution has been investigated for the first time. The complexes were characterized by UV/vis, IR and NMR spectroscopy and X-ray crystallographic studies of the compounds fac-[Mn(CO)3(Bipy)(H2O)][CF3SO3] () and fac-[Mn(CO)3(Phen)(H2O)][CF3SO3] () are reported. A two order-of-magnitude of activation for the methanol substitution is induced as manifested by the second order rate constants with (N,N'-Bid) < (N,O-Bid). Forward and reverse rate and stability constants from slow and stopped-flow UV/vis measurements (k1, M(-1) s(-1); k-1, s(-1); K1, M(-1)) for pyridine as entering nucleophile are as follows: fac-[Mn(CO)3(Phen)(CH3OH)](+) (2.39 ± 5) × 10(-3), (1.5 ± 0.3) × 10(-5), 159 ± 32; fac-[Mn(CO)3(2,4-QuinH)(CH3OH)] (4.5 ± 0.2), (4 ± 1) × 10(-2), 113 ± 29. Activation parameters (ΔH, kJ mol(-1); ΔS, J K(-1) mol(-1)) from Eyring plots for entering nucleophiles as indicated are as follows: fac-[Mn(CO)3(Phen)(CH3OH)](+) (bromide ions) 66.7 ± 0.6, -27 ± 2; (pyridine) 80 ± 3, -25 ± 11; fac-[Mn(CO)3(Pico)(CH3OH)] (bromide ions) 68 ± 2, -24 ± 5. A dissociative interchange mechanism is proposed.

18F-FAC PET selectively images hepatic infiltrating CD4 and CD8 T cells in a mouse model of autoimmune hepatitis.

PubMed

Salas, Jessica R; Chen, Bao Ying; Wong, Alicia; Cheng, Donghui; Van Arnam, John S; Witte, Owen N; Clark, Peter M

2018-04-26

Immune cell-mediated attack on the liver is a defining feature of autoimmune hepatitis and hepatic allograft rejection. Despite an assortment of diagnostic tools, invasive biopsies remain the only method for identifying immune cells in the liver. We evaluated whether PET imaging with radiotracers that quantify immune activation ( 18 F-FDG and 18 F-FAC) and hepatocyte biology ( 18 F-DFA) can visualize and quantify hepatic infiltrating immune cells and hepatocyte inflammation, respectively, in a preclinical model of autoimmune hepatitis. Methods: Mice treated with Concanavalin A (ConA) to induce a model of autoimmune hepatitis or vehicle were imaged with 18 F-FDG, 18 F-FAC, and 18 F-DFA PET. Immunohistochemistry, digital autoradiography, and ex vivo accumulation assays were used to localize areas of altered radiotracer accumulation in the liver. For comparison, mice treated with an adenovirus to induce a viral hepatitis or vehicle were imaged with 18 F-FDG, 18 F-FAC, and 18 F-DFA PET. 18 F-FAC PET was performed on mice treated with ConA, and vehicle or dexamethasone. Biopsy samples of patients suffering from autoimmune hepatitis were immunostained for deoxycytidine kinase (dCK). Results: Hepatic accumulation of 18 F-FDG and 18 F-FAC was 173% and 61% higher, respectively, and hepatic accumulation of 18 F-DFA was 41% lower in a mouse model of autoimmune hepatitis compared to control mice. Increased hepatic 18 F-FDG accumulation was localized to infiltrating leukocytes and inflamed sinusoidal endothelial cells, increased hepatic 18 F-FAC accumulation was concentrated in infiltrating CD4 and CD8 cells, and decreased hepatic 18 F-DFA accumulation was apparent in hepatocytes throughout the liver. In contrast, viral hepatitis increased hepatic 18 F-FDG accumulation by 109% and decreased hepatic 18 F-DFA accumulation by 20% but had no effect on hepatic 18 F-FAC accumulation (non-significant 2% decrease). 18 F-FAC PET provided a non-invasive biomarker of the efficacy of dexamethasone for treating the autoimmune hepatitis model. Infiltrating leukocytes in liver biopsy samples from patients suffering from autoimmune hepatitis express high levels of dCK, a rate-limiting enzyme in the accumulation of 18 F-FAC. Conclusion: Our data suggests that PET can be used to non-invasively visualize activated leukocytes and inflamed hepatocytes in a mouse model of autoimmune hepatitis. Copyright © 2018 by the Society of Nuclear Medicine and Molecular Imaging, Inc.

Electrodynamic parameters in the nighttime sector during auroral substorms

NASA Technical Reports Server (NTRS)

Fujii, R.; Hoffman, R. A.; Anderson, P. C.; Craven, J. D.; Sugiura, M.; Frank, L. A.; Maynard, N. C.

1994-01-01

The characteristics of the large-scale electrodynamic parameters, field-aligned currents (FACs), electric fields, and electron precipitation, which are associated with auroral substorm events in the nighttime sector, have been obtained through a unique analysis which places the ionospheric measurements of these parameters into the context of a generic substorm determined from global auroral images. A generic bulge-type auroral emission region has been deduced from auroral images taken by the Dynamics Explorer 1 (DE 1) satellite during a number of isolated substorms, and the form has been divided into six sectors, based on the peculiar emission characteristics in each sector: west of bulge, surge horn, surge, middle surge, eastern bulge, and east of bulge. By comparing the location of passes of the Dynamics Explorer 2 (DE 2) satellite to the simultaneously obtained auroral images, each pass is placed onto the generic aurora. The organization of DE 2 data in this way has systematically clarified peculiar characteristics in the electrodynamic parameters. An upward net current mainly appears in the surge, with little net current in the surge horn and the west of bulge. The downward net current is distributed over wide longitudinal regions from the eastern bulge to the east of bulge. Near the poleward boundary of the expanding auroral bulge, a pair of oppositely directed FAC sheets is observed, with the downward FAC on the poleward side. This downward FAC and most of the upward FAC in the surge and the middle surge are assoc iated with narrow, intense antisunwqard convection, corresponding to an equatorward directed spikelike electric field. This pair of currents decreases in amplitude and latitudinal width toward dusk in the surge and the west of bulge, and the region 1 and 2 FACs become embedded in the sunward convection region. The upward FAC region associated with the spikelike field on the poleward edge of the bulge coincides well with intense electron precipitation and aurora appearing in this western and poleward protion of the bulge. The convection reversal is sharp in the west of bulge and surge horn sectors, and near the high-latitude boundary of the upward region 1, with a near stagnation region often extending over a large interval of latitude. In the eastern bulge and east of bulge sectors, the region 1 and 2 FACs are located in the sunward convection region, while a spikelike electric field occasionally appears poleward of the aurora but usually not associated with a pair of FAC sheets. In the eastern bulge, magnetic field data show complicated FAC distributions which correspond to current segments and filamentary currents.

Simultaneous prenoon and postnoon observations of three field-aligned current systems from Viking and DMSP-F7

NASA Technical Reports Server (NTRS)

Ohtani, S.; Potemra, T. A.; Newell, P. T.; Zanetti, L. J.; Iijima, T.; Watanabe, M.; Yamauchi, M.; Elphinstone, R. D.; De La Beauijardie, O.; Blomberg, L. G.

1995-01-01

The spatial structure of dayside large-scale field-aligned current (FAC) systems is examined by using Viking and Defense Meteorological Satellite Program-F7 (DMSP-F7) data. We focus on four events in which the satellites simultaneously observed postnoon and prenoon three FAC systems: the region 2, the region 1, and the mantle (referred to as midday region O) systems, from equatorward to poleward. These events provide the most solid evidence to date that the midday region O system is a separate and unique FAC system, and is not an extension of the region 1 system from other local times. The events are examined comprehensively by making use of a mulit-instrumental data set, which includes magnetic field, particle flux, electric field, auroral UV image data from the satellites, and the Sondrestrom convection data. The results are summarized as follows: (1) Region 2 currents flow mostly in the central plasma sheet (CPS) precipitation region, often overlapping with the boundary plasma sheet (BPD) at their poleward edge. (2) The region 1 system is located in the core part of the auroral oval and is confined in a relatively narrow range in latitude which includes the convection reversal. The low-latitude boundary layer, possibly including the outer part of the plasma sheet, and the external cusp are the major source regions of dayside region 1 currents. (2) Midday region O currents flow on open field lines and are collocated with the shear of antisunward convection flows with velocites decreasing poleward. On the basis of these results we support the view that both prenoon and postnoon current systems consist of the three-sheet structure when the disctortion ofthe convection pattern associated with interplanetary magnetic field (IMF) B(sub Y) is small and both morningside and eveningside convection cells are crescent-shaped. We also propose that the midday region O and a part of the region 1 systems are closely coupled to the same source.

Damage Control Resuscitation: Directly Addressing the Early Coagulopathy of Trauma

DTIC Science & Technology

2007-02-01

FACS, Jay Johannigman, MD, FS, FACS, Peter Mahoney, FRCA, RAMC , Sumeru Mehta, MD, E. Darrin Cox, MD, FACS, Michael J. Gehrke, MD, Greg J. Beilman, MD...opportunity to formally evaluate the immediate and direct treatment of the coagulopathy of trauma is available. Submitted for publication September 22, 2006...the results were not evaluated in randomized human trials.33–35 Additionally, the potential benefits of mitigating ischemia-induced reperfusion injury

Syntheses and multi-NMR study of fac- and mer-OsO(3)F(2)(NCCH(3)) and the X-ray crystal structure (n = 2) and Raman spectrum (n = 0) of fac-OsO(3)F(2)(NCCH(3)).nCH(3)CN.

PubMed

Hughes, Michael J; Gerken, Michael; Mercier, Hélène P A; Schrobilgen, Gary J

2010-06-07

Dissolution of the infinite chain polymer, (OsO(3)F(2))(infinity), in CH(3)CN solvent at -40 degrees C followed by solvent removal under vacuum at -40 degrees C yielded fac-OsO(3)F(2)(NCCH(3)).nCH(3)CN (n >/= 2). Continued pumping at -40 degrees C with removal of uncoordinated CH(3)CN yielded fac-OsO(3)F(2)(NCCH(3)). Both fac-OsO(3)F(2)(NCCH(3)).nCH(3)CN and fac-OsO(3)F(2)(NCCH(3)) are yellow-brown solids and were characterized by low-temperature (-150 degrees C) Raman spectroscopy. The crystal structure (-173 degrees C) of fac-OsO(3)F(2)(NCCH(3)).2CH(3)CN consists of two co-crystallized CH(3)CN molecules and a pseudo-octahedral OsO(3)F(2).NCCH(3) molecule in which three oxygen atoms are in a facial arrangement and CH(3)CN is coordinated trans to an oxygen atom in an end-on fashion. The Os---N bond length (2.205(3) A) is among the shortest M---N adduct bonds observed for a d(0) transition metal oxide fluoride. The (19)F NMR spectrum of (OsO(3)F(2))(infinity) in CH(3)CN solvent (-40 degrees C) is a singlet (-99.6 ppm) corresponding to fac-OsO(3)F(2)(NCCH(3)). The (1)H, (15)N, (13)C, and (19)F NMR spectra of (15)N-enriched OsO(3)F(2)(NCCH(3)) were recorded in SO(2)ClF solvent (-84 degrees C). Nitrogen-15 enrichment resulted in splitting of the (19)F resonance of fac-OsO(3)F(2)((15)NCCH(3)) into a doublet ((2)J((15)N-(19)F), 21 Hz). In addition, a doublet of doublets ((2)J((19)F(ax)-(19)F(eq)), 134 Hz; (2)J((15)N-(19)F(eq)), 18 Hz) and a doublet ((2)J((19)F(ax)-(19)F(eq)), 134 Hz) were observed in the (19)F NMR spectrum that have been assigned to mer-OsO(3)F(2)((15)NCCH(3)); however, coupling of (15)N to the axial fluorine-on-osmium environment could not be resolved. The nitrogen atom of CH(3)CN is coordinated trans to a fluorine ligand in the mer-isomer. Quantum-chemical calculations at the SVWN and B3LYP levels of theory were used to calculate the energy-minimized gas-phase geometries, vibrational frequencies of fac- and mer-OsO(3)F(2)(NCCH(3)) and of CH(3)CN. The relative stabilities of the mer- and fac-isomers have been determined and are in accordance with the solution NMR assignments.

Coordination modes of multidentate ligands in fac-[Re(CO)(3)(polyaminocarboxylate)] analogues of (99m)Tc radiopharmaceuticals. dependence on aqueous solution reaction conditions.

PubMed

Lipowska, Malgorzata; He, Haiyang; Xu, Xiaolong; Taylor, Andrew T; Marzilli, Patricia A; Marzilli, Luigi G

2010-04-05

We study Re analogues of (99m)Tc renal agents to interpret previous results at the (99m)Tc tracer level. The relative propensities of amine donors versus carboxylate oxygen donors of four L = polyaminocarboxylate ligands to coordinate in fac-[Re(I)(CO)(3)L](n) complexes were assessed by examining the reaction of fac-[Re(I)(CO)(3)(H(2)O)(3)](+) under conditions differing in acidity and temperature. All four L [N,N-bis-(2-aminoethyl)glycine (DTGH), N,N-ethylenediaminediacetic acid, diethylenetriamine-N-malonic acid, and diethylenetriamine-N-acetic acid] can coordinate as tridentate ligands while creating a dangling chain terminated in a carboxyl group. Dangling carboxyl groups facilitate renal clearance in fac-[(99m)Tc(I)(CO)(3)L](n) agents. Under neutral conditions, the four ligands each gave two fac-[Re(I)(CO)(3)L](n) products with HPLC traces correlating well with known traces of the fac-[(99m)Tc(I)(CO)(3)L](n) mixtures. Such mixtures are common in renal agents because the needed dangling carboxyl group can compete for a coordination site. However, the HPLC separations needed to assess the biodistribution of a single tracer are impractical in a clinical setting. One goal in investigating this Re chemistry is to identify conditions for avoiding this problem of mixtures in preparations of fac-[(99m)Tc(I)(CO)(3)L](n) renal tracers. After separation and isolation of the fac-[Re(I)(CO)(3)L](n) products, NMR analysis of all products and single crystal X-ray crystallographic analysis of both DTGH products, as well as one product each from the other L, allowed us to establish coordination mode unambiguously. The product favored in acidic conditions has a dangling amine chain and more bound oxygen. The product favored in basic conditions has a dangling carboxyl chain and more bound nitrogen. At the elevated temperatures used for simulating tracer preparation, equilibration was facile (ca. 1 h or less), allowing selective formation of one product by utilizing acidic or basic conditions. The results of this fundamental study offer protocols and guidance useful for the design and preparation of fac-[(99m)Tc(I)(CO)(3)L](n) agents consisting of a single tracer.

The effect of the stroke etiology on functional improvement in our geriatric hemiplegic patients.

PubMed

Nakipoğlu-Yüzer, Güldal F; Doğan-Aslan, Meryem; Doğan, Asuman; Ozgirgin, Neşe

2010-05-01

We aimed to determine the effect of the cerebrovascular accident etiology in the geriatric patients with hemiplegia included in our physical medicine and rehabilitation program on functional improvement. A total of 46 geriatric patients with hemiplegia attending the inpatient physical medicine and rehabilitation program were included in the study. The patients were divided into two groups-thromboembolic vessel disease (TEVD) and intracerebral hemorrhage (ICH)-depending on the cerebrovascular accident etiology. The daily living activities of the patients in both groups were evaluated using the Barthel Index (BI) and the ambulation levels were evaluated using the Functional Ambulation Classification (FAC) at admittance and at discharge from hospital. There was a statistically significant difference between admission and discharge BI values in both groups. There was no significant difference between the admission and discharge BI scores of the TEVD and ICH groups. For both groups, on admission there were 19 (82.5%) patients at the FAC 0, 1, and 2 levels, and 4 (17.3%) patients at the FAC 3 and 4 levels. On discharge there were 11 (47.8%) patients in the TEVD group at the FAC 0 and 2 levels, and 12 (52.1%) patients at the FAC 3, 4, and 5 levels; whereas in the ICH group there were 8 (34.7%) patients at the FAC 0, 1, and 2 levels, and 15 (65.2%) patients at the FAC 3, 4, and 5 levels. We found that the disease etiology did not influence the rehabilitation results for our geriatric patients with hemiplegia attending a physical medicine and rehabilitation program following TEVD or ICH. Copyright (c) 2010 National Stroke Association. Published by Elsevier Inc. All rights reserved.

Perioperative echocardiography-derived right ventricle function parameters and early outcomes after tetralogy of Fallot repair in mid-childhood: a single-center, prospective observational study.

PubMed

Raj, Ravi; Puri, Goverdhan Dutt; Jayant, Aveek; Thingnam, Shyam Kumar Singh; Singh, Rana Sandip; Rohit, Manoj Kumar

2016-11-01

Right ventricular (RV) function alterations are invariably present in all patients after tetralogy of Fallot (TOF) repair. Unlike the developed world where most of the patients with TOF are corrected in infancy, average age of presentation and thus surgery for these patients in the developing world may be higher. We aimed to study the correlation between RV function parameters such as tricuspid annular peak systolic excursion (TAPSE), fractional area change (FAC), and tricuspid annular peak systolic velocity (S') with early outcome variables after intracardiac repair for TOF. Fifty patients with a preoperative diagnosis of tetralogy of Fallot scheduled for corrective surgery were included in this single-center, prospective observational study. A preoperative transthoracic echocardiogram was performed to measure RV function parameters (FAC0, TAPSE0, S'0). Transthoracic echocardiography was repeated postoperatively to measure FAC1, TAPSE1, S'1 (day 1) and FAC2, TAPSE2, and S'2 (day 3). The relationship between preoperative and postoperative RV function parameters with in-hospital mortality, duration of mechanical ventilation, and intensive care unit stay was studied. The median age of patients was 6 years (range 1-14 years). Multiple stepwise logistic regression analysis showed RV FAC as best predictor of clinical outcome. Area under the receiver operating characteristic curve for postoperative RV function parameters, that is, FAC, TAPSE, and S' to predict early or delayed recovery was 0.944, 0.875, and 0.655, respectively. Among the RV function parameters studied, RV FAC best predicted the early outcome variables after TOF repair, followed by TAPSE while lateral tricuspid annular velocity S' being the least predictive. © 2016, Wiley Periodicals, Inc.

Effect of Preload Alterations on Left Ventricular Systolic Parameters Including Speckle-Tracking Echocardiography Radial Strain During General Anesthesia.

PubMed

Weber, Ulrike; Base, Eva; Ristl, Robin; Mora, Bruno

2015-08-01

Frequently used parameters for evaluation of left ventricular systolic function are load-sensitive. However, the impact of preload alterations on speckle-tracking echocardiographic parameters during anesthesia has not been validated. Therefore, two-dimensional (2D) speckle-tracking echocardiography radial strain (RS) was assessed during general anesthesia, simulating 3 different preload conditions. Single-center prospective observational study. University hospital. Thirty-three patients with normal left ventricular systolic function undergoing major surgery. Transgastric views of the midpapillary level of the left ventricle were acquired at 3 different positions. Fractional shortening (FS), fractional area change (FAC), and 2D speckle-tracking echocardiography RS were analyzed in the transgastric midpapillary view. Considerable correlation above 0.5 was found for FAC and FS in the zero and Trendelenburg positions (r = 0.629, r = 0.587), and for RS and FAC in the anti-Trendelenburg position (r = 0.518). In the repeated-measures analysis, significant differences among the values measured at the 3 positions were found for FAC and FS. For FAC, there were differences up to 2.8 percentage points between the anti-Trendelenburg position and the other 2 positions. For FS, only the difference between position zero and anti-Trendelenburg was significant, with an observed change of 1.66. Two-dimensional RS was not significantly different at all positions, with observed changes below 1 percentage point. Alterations in preload did not result in clinically relevant changes of RS, FS, or FAC. Observed changes for RS were smallest; however, the variation of RS was larger than that of FS or FAC. Copyright © 2015 Elsevier Inc. All rights reserved.

A study of the radiosynthesis of fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ and its application in labeling 1,2,3-triazole analogs obtained by click chemistry.

PubMed

Wang, Cheng; Zhou, Wei; Yu, Junfeng; Zhang, Lan; Wang, Ni

2012-01-01

To optimize the conditions for the preparation of the organometallic precursor fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ and to synthesize the radiolabeling compounds of tricarbonyl rhenium. 1,2,3-Triazole analogs were synthesized by click chemistry and labeled with fac-[ReCO₃(H₂O)₃]Br and fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺. The aim was to improve the methods for the synthesis of ¹⁸⁸Re-labeled radiopharmaceuticals for therapy. With potassium boranocarbonate as the CO source and ammonia borane as the reducing agent, fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ was synthesized, and the click chemistry method was used to prepare the tricarbonyl rhenium complex. At the optimal reaction condition (the amounts of K₂[H₃BCO₂] and BH₃·NH₃ are 5 and 5 mg, respectively; reaction temperature is 75°C; and reaction time is 15 min), the radiochemical yields were 90%, and the labeling yield of bis(pyridin-2-ylmethyl) amine with fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ was more than 99% in 1 h at 75°C; the conjugation yields of triazole analog obtained by click chemistry with 'cold' and 'radio' tricarbonyl rhenium were more than 80%. The organometallic precursor fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ was prepared under optimal reaction conditions with a yield of 90%, and the triazole analogs synthesized by click chemistry were suitable ligands for tricarbonyl rhenium.

Antinociceptive Activity of Borreria verticillata: In vivo and In silico Studies

PubMed Central

Silva, Rosa H. M.; Lima, Nathália de Fátima M.; Lopes, Alberto J. O.; Vasconcelos, Cleydlenne C.; de Mesquita, José W. C.; de Mesquita, Ludmilla S. S.; Lima, Fernando C. V. M.; Ribeiro, Maria N. de S.; Ramos, Ricardo M.; Cartágenes, Maria do Socorro de S.; Garcia, João B. S.

2017-01-01

Borreria verticillata (L.) G. Mey. known vassourinha has antibacterial, antimalarial, hepatoprotective, antioxidative, analgesic, and anti-inflammatory, however, its antinociceptive action requires further studies. Aim of the study evaluated the antinociceptive activity of B. verticillata hydroalcoholic extract (EHBv) and ethyl acetate fraction (FAc) by in vivo and in silico studies. In vivo assessment included the paw edema test, writhing test, formalin test and tail flick test. Wistar rats and Swiss mice were divided into 6 groups and given the following treatments oral: 0.9% NaCl control group (CTRL), 10 mg/kg memantine (MEM), 10 mg/kg indomethacin (INDO), 500 mg/kg EHBv (EHBv 500), 25 mg/kg FAc (FAc 25) and 50 mg/kg FAc (FAc 50). EHBv, FAc 25 and 50 treatments exhibited anti-edematous and peripheral antinociceptive effects. For in silico assessment, compounds identified in FAc were subjected to molecular docking with COX-2, GluN1a and GluN2B. Ursolic acid (UA) was the compound with best affinity parameters (binding energy and inhibition constant) for COX-2, GluN1a, GluN2B, and was selected for further analysis with molecular dynamics (MD) simulations. In MD simulations, UA exhibited highly frequent interactions with residues Arg120 and Glu524 in the COX-2 active site and NMDA, whereby it might prevent COX-2 and NMDA receptor activation. Treatment with UA 10 mg/Kg showed peripheral and central antinociceptive effect. The antinociceptive effect of B. verticillata might be predominantly attributed to peripheral actions, including the participation of anti-inflammatory components. Ursolic acid is the main active component and seems to be a promising source of COX-2 inhibitors and NMDA receptor antagonists. PMID:28588488

Long-term outcomes of phakic patients with diabetic macular oedema treated with intravitreal fluocinolone acetonide (FAc) implants

PubMed Central

Yang, Y; Bailey, C; Holz, F G; Eter, N; Weber, M; Baker, C; Kiss, S; Menchini, U; Ruiz Moreno, J M; Dugel, P; Lotery, A

2015-01-01

Purpose Diabetic macular oedema (DMO) is a leading cause of blindness in working-age adults. Slow-release, nonbioerodible fluocinolone acetonide (FAc) implants have shown efficacy in the treatment of DMO; however, the National Institute for Health and Care Excellence recommends that FAc should be used in patients with chronic DMO considered insufficiently responsive to other available therapies only if the eye to be treated is pseudophakic. The goal of this analysis was to examine treatment outcomes in phakic patients who received 0.2 μg/day FAc implant. Methods This analysis of the phase 3 FAME (Fluocinolone Acetonide in Diabetic Macular Edema) data examines the safety and efficacy of FAc implants in patients who underwent cataract extraction before (cataract before implant (CBI) group) or after (cataract after implant (CAI) group) receiving the implant. The data were further examined by DMO duration. Results Best corrected visual acuity (BCVA) after 36 months was comparable in the CAI and CBI groups. Both the percentage of patients gaining ≥3 lines of vision and mean change in BCVA letter score were numerically greater in the CAI group. In addition, most patients who underwent cataract surgery experienced a net gain in BCVA from presurgery baseline as well as from original study baseline. Conclusions These data support the use of 0.2 μg/day FAc implants in phakic as well as in pseudophakic patients. These findings will serve as a pilot for design of future studies to evaluate the potential protective effect of FAc implants before cataract surgery in patients with DMO and cataract. PMID:26113503

Subauroral polarization stream on the outer boundary of the ring current during an energetic ion injection event

NASA Astrophysics Data System (ADS)

Yuan, Zhigang; Qiao, Zheng; Li, Haimeng; Huang, Shiyong; Wang, Dedong; Yu, Xiongdong; Yu, Tao

2017-04-01

Subauroral polarization stream (SAPS) electric field can play an important role in the coupling between the inner magnetosphere and ionosphere; however, the production mechanism of SAPS has not been yet solved. During an energetic ion injection event on 26 March 2004, at latitudes lower than the equatorward boundaries of precipitating plasma sheet electrons and ions, the Defense Meteorological Satellite Program (DMSP) F13 satellite simultaneously observed a strong SAPS with the peak velocity of 1294 m/s and downward flowing field-aligned currents (FACs). Conjugate observations of DMSP F13 and NOAA 15 satellites have shown that FACs flowing into the ionosphere just lie in the outer boundary of the ring current (RC). The downward flowing FACs were observed in a region of positive latitudinal gradients of the ion energy density, implying that the downward flowing FACs are more likely linked to the azimuthal gradient than the radial gradient of the RC ion pressure. Our result demonstrates that RC ion pressure gradients on the outer boundary of the RC in the evening sector during energetic ion injection events can lead to downward flowing FACs so as to cause strong SAPS in condition of low ionospheric conductivities.