Sample records for cold storage building

  1. Aquifer Thermal Energy Storage for Seasonal Thermal Energy Balance

    NASA Astrophysics Data System (ADS)

    Rostampour, Vahab; Bloemendal, Martin; Keviczky, Tamas

    2017-04-01

    Aquifer Thermal Energy Storage (ATES) systems allow storing large quantities of thermal energy in subsurface aquifers enabling significant energy savings and greenhouse gas reductions. This is achieved by injection and extraction of water into and from saturated underground aquifers, simultaneously. An ATES system consists of two wells and operates in a seasonal mode. One well is used for the storage of cold water, the other one for the storage of heat. In warm seasons, cold water is extracted from the cold well to provide cooling to a building. The temperature of the extracted cold water increases as it passes through the building climate control systems and then gets simultaneously, injected back into the warm well. This procedure is reversed during cold seasons where the flow direction is reversed such that the warmer water is extracted from the warm well to provide heating to a building. From the perspective of building climate comfort systems, an ATES system is considered as a seasonal storage system that can be a heat source or sink, or as a storage for thermal energy. This leads to an interesting and challenging optimal control problem of the building climate comfort system that can be used to develop a seasonal-based energy management strategy. In [1] we develop a control-oriented model to predict thermal energy balance in a building climate control system integrated with ATES. Such a model however cannot cope with off-nominal but realistic situations such as when the wells are completely depleted, or the start-up phase of newly installed wells, etc., leading to direct usage of aquifer ambient temperature. Building upon our previous work in [1], we here extend the mathematical model for ATES system to handle the above mentioned more realistic situations. Using our improved models, one can more precisely predict system behavior and apply optimal control strategies to manage the building climate comfort along with energy savings and greenhouse gas reductions. [1] V. Rostampour and T. Keviczky, "Probabilistic Energy Management for Building Climate Comfort in Smart Thermal Grids with Seasonal Storage Systems," arXiv [math.OC], 10-Nov-2016.

  2. A Lithium Bromide Absorption Chiller with Cold Storage

    DTIC Science & Technology

    2011-01-15

    Research ABSTRACT A LiBr -based absorption chiller can use waste heat or solar energy to produce useful space cooling for small buildings...high wa- ter consumption for heat rejection to the ambient. To alleviate these issues, a novel LiBr - based absorption chiller with cold storage is...proposed in this study. The cold storage includes tanks for storing liquid water and LiBr solution, associated piping, and control devices. The cold

  3. 21. Detail of typical refrigeration unit in the southwest corner ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. Detail of typical refrigeration unit in the southwest corner of the fruit and vegetable storage room - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  4. 20. Detail of 8" square solid wood column at fruit ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Detail of 8" square solid wood column at fruit and vegetable storage room; note ledger plates bolted to top of column - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  5. Holocene cold storage practices on the eastern Snake River Plain: A risk-mitigation strategy for lean times

    DOE PAGES

    Byers, David A.; Henrikson, L. Suzann; Breslawski, Ryan P.

    2016-06-04

    Previous archaeological research in southern Idaho has suggested that climate change over the past 8000 years was not dramatic enough to alter long-term subsistence practices in the region. However, recent isotopic analyses of bison remains from cold storage caves on the Snake River Plain contest this hypothesis. Our results, when examined against an archaeoclimate model, suggest that cold storage episodes coincided with drier, warmer phases that likely reduced forage and water, and thus limited the availability of bison on the open steppe. Within this context we build a risk model to illustrate how environment might have motivated cold storage behaviors.more » Caching bison in cold lava tubes would have mitigated both intra-annual and inter-annual food shortages under these conditions. This analysis also suggests that skeletal fat, more than meat, may have influenced the selection, transport and storage of bison carcass parts. We deciphered when and how cold storage caves which was used to provide a more comprehensive understanding of foraging behaviors in a broad range of hunting-gathering economies.« less

  6. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Byers, David A.; Henrikson, L. Suzann; Breslawski, Ryan P.

    Previous archaeological research in southern Idaho has suggested that climate change over the past 8000 years was not dramatic enough to alter long-term subsistence practices in the region. However, recent isotopic analyses of bison remains from cold storage caves on the Snake River Plain contest this hypothesis. Our results, when examined against an archaeoclimate model, suggest that cold storage episodes coincided with drier, warmer phases that likely reduced forage and water, and thus limited the availability of bison on the open steppe. Within this context we build a risk model to illustrate how environment might have motivated cold storage behaviors.more » Caching bison in cold lava tubes would have mitigated both intra-annual and inter-annual food shortages under these conditions. This analysis also suggests that skeletal fat, more than meat, may have influenced the selection, transport and storage of bison carcass parts. We deciphered when and how cold storage caves which was used to provide a more comprehensive understanding of foraging behaviors in a broad range of hunting-gathering economies.« less

  7. 28. Photographic copy of the Post Engineer drawing (original is ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Photographic copy of the Post Engineer drawing (original is located at Fort Hood) building section & wall sections, plan number PE 1264.1 - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  8. 29. Photographic copy of the Post Engineer drawing (original is ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    29. Photographic copy of the Post Engineer drawing (original is located at Fort Hood) building section & details, plan number PE 1264.2 - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  9. 1. General oblique view of north and east sides, view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. General oblique view of north and east sides, view to southwest, showing main loading docks - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  10. 2. General oblique view of north loading dock showing loading ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. General oblique view of north loading dock showing loading docks with doors opening into refrigerated rooms - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  11. Fort Hood Building and Landscape Inventory with WWII and Cold War Context

    DTIC Science & Technology

    2007-03-01

    barracks, 1970s (NARA)........................................................... 112 Figure 37. Palmer Movie Theater (NARA...revised 1953) showing layout of Hood Village and trailer park (Fort Hood...arms ammunition storage building #92012 (ERDC-CERL, 2004). ......... 260 Figure 163: Radio reception building #92063 (ERDC-CERL, 2004

  12. 10. BUILDING: SECOND FLOOR (East Section), VIEW SOUTH: EAST, SOUTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. BUILDING: SECOND FLOOR (East Section), VIEW SOUTH: EAST, SOUTH AND WEST WALLS OF COLD STORAGE, ALSO SHOWING REMNANTS OF COOLING PIPES - Boston Beer Company, 225-249 West Second Street, South Boston, Suffolk County, MA

  13. CONTEXTUAL AERIAL VIEW OF "COLD" NORTH HALF OF MTR COMPLEX. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CONTEXTUAL AERIAL VIEW OF "COLD" NORTH HALF OF MTR COMPLEX. CAMERA FACING EASTERLY. FOREGROUND CORNER CONTAINS OIL STORAGE TANKS. WATER TANKS AND WELL HOUSES ARE BEYOND THEM TO THE LEFT. LARGE LIGHT-COLORED BUILDING IN CENTER OF VIEW IS STEAM PLANT. DEMINERALIZER AND WATER STORAGE TANK ARE BEYOND. SIX-CELL COOLING TOWER AND ITS PUMP HOUSE ARE ABOVE IT IN VIEW. SERVICE BUILDINGS INCLUDING CANTEEN ARE ON NORTH SIDE OF ROAD. "EXCLUSION" AREA IS BEYOND ROAD. COMPARE LOCATION OF EXCLUSION-AREA GATE WITH PHOTO ID-33-G-202. INL NEGATIVE NO. 3608. Unknown Photographer, 10/30/1951 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  14. 31. Photographic copy of the Post Engineer drawing (original is ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. Photographic copy of the Post Engineer drawing (original is located at Fort Hood) concrete dock details, plan number PE 1265.1 - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  15. 34. Photographic copy of the Post Engineer drawing (original is ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    34. Photographic copy of the Post Engineer drawing (original is located at Fort Hood) electrical riser plan, plan number PE 1268.2 - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  16. 33. Photographic copy of the Post Engineer drawing (original is ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    33. Photographic copy of the Post Engineer drawing (original is located at Fort Hood) electric plan & light fixture schedule, plan number PE 1268.1 - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  17. 32. Photographic copy of the Post Engineer drawing (original is ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. Photographic copy of the Post Engineer drawing (original is located at Fort Hood) compressor, evaporative condenser & unit cooler schedules, plan number PE 1268 - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  18. Preliminary investigation of thermal behaviour of PCM based latent heat thermal energy storage

    NASA Astrophysics Data System (ADS)

    Pop, Octavian G.; Fechete Tutunaru, Lucian; Bode, Florin; Balan, Mugur C.

    2018-02-01

    Solid-liquid phase change is used to accumulate and release cold in latent heat thermal energy storage (LHTES) in order to reduce energy consumption of air cooling system in buildings. The storing capacity of the LHTES depends greatly on the exterior air temperatures during the summer nights. One approach in intensifying heat transfer is by increasing the air's velocity. A LHTES was designed to be integrated in the air cooling system of a building located in Bucharest, during the month of July. This study presents a numerical investigation concerning the impact of air inlet temperatures and air velocity on the formation of solid PCM, on the cold storing capacity and energy consumption of the LHTES. The peak amount of accumulated cold is reached at different air velocities depending on air inlet temperature. For inlet temperatures of 14°C and 15°C, an increase of air velocity above 50% will not lead to higher amounts of cold being stored. For Bucharest during the hottest night of the year, a 100 % increase in air velocity will result in 5.02% more cold being stored, at an increase in electrical energy consumption of 25.30%, when compared to the reference values.

  19. Limited energy study. Thermal storage at Central Chilled Water Plant, Fort Leonard Wood, Missouri. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-05-31

    The Scope of Work called for the study of the economic feasibility of providing a cold thermal storage system at the central chiller plant serving the Fort Leonard Wood 600 Area in order to reduce electrical demand charges. In the Entry Interview, Mr. Doug Cage requested that the analysis include the potential for expansion of such a system to serve the 700 and 800 Areas as well. It was agreed that this would be done if the analysis indicated that a cold thermal storage system would be economically feasible for Area 600. The 600 Area study area is comprised ofmore » two different build types, mess halls and barracks. The mess halls are all essentially identical with the exception that site orientation varies by building. The same is true for the barracks buildings. A baseline case was calculated under the basis that the future chilled water plant for the area under analysis would be served by a centrifugal chiller. This was done because there is no existing baseline condition against which thermal storage systems may be compared. The existing chiller serves Area 600 plus a portion of Area 700. In addition, its age is such that it is reasonable to expect that it will be replaced in the near future.« less

  20. Unsteady Stored Heat Behavior in Building Frame of Reinforced Concrete Structure Type Cold Storage

    NASA Astrophysics Data System (ADS)

    Nomura, Tomohiro; Murakami, Yuji; Uchikawa, Motoyuki

    The time variation of temperature in the reinforced concrete frame with an internal insulation or with an external insulation and the unsteady stored heat behavior, which results from the thermal mass of the concrete frame, have been investigated. The experiments with the concrete models and the measurements of the heat flux through the practical cold storage were performed. The experimental results under the unsteady condition showed great difference of the stored heat behavior between the internal insulation type and the external type. In addition, it was shown that the external insulation frame was useful for heat storage. The simulation method with two dimentional unsteady FEM was introduced for easily analyzing the stored heat behavior problems of the practical cold storages, which had various specifications in design. The calculated results of the heat flux and temperature in the concrete frame agreed with the experiments approximately. From these results, the suggestions for the design of the insulation wall under the unsteady condition were given.

  1. 25. INTERIOR VIEW TO THE SOUTHWEST OF ROOM 109, THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. INTERIOR VIEW TO THE SOUTHWEST OF ROOM 109, THE WARM AND COLD STORAGE ROOM. - Nevada Test Site, Pluto Facility, Disassembly Building, Area 26, Wahmonie Flats, Cane Spring Road, Mercury, Nye County, NV

  2. 14. Interior view of the first floor, facing southwest, and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Interior view of the first floor, facing southwest, and showing elements of the cold storage refrigeration system attached to the ceiling. - Armour & Company Building, 100 Harris Avenue, Providence, Providence County, RI

  3. 26. Photocopy of photograph taken by W. Terry Averbeck, date ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. Photocopy of photograph taken by W. Terry Averbeck, date unknown. VIEW, LOOKING NORTHWEST, OF SECOND STREET BETWEEN SCOTT AND COURT STREETS, SHOWING PORTIONS OF THE ICE STORAGE BUILDING AND ORIGINAL SHIPPING AREA, NOW USED AS BAGGING/CRUSHING AREA (8x10' enlargement from 4x5' negative) - Champion Ice Manufacturing & Cold Storage Company, 40 East Second Street, Covington, Kenton County, KY

  4. Performance analysis of phase-change material storage unit for both heating and cooling of buildings

    NASA Astrophysics Data System (ADS)

    Waqas, Adeel; Ali, Majid; Ud Din, Zia

    2017-04-01

    Utilisation of solar energy and the night ambient (cool) temperatures are the passive ways of heating and cooling of buildings. Intermittent and time-dependent nature of these sources makes thermal energy storage vital for efficient and continuous operation of these heating and cooling techniques. Latent heat thermal energy storage by phase-change materials (PCMs) is preferred over other storage techniques due to its high-energy storage density and isothermal storage process. The current study was aimed to evaluate the performance of the air-based PCM storage unit utilising solar energy and cool ambient night temperatures for comfort heating and cooling of a building in dry-cold and dry-hot climates. The performance of the studied PCM storage unit was maximised when the melting point of the PCM was ∼29°C in summer and 21°C during winter season. The appropriate melting point was ∼27.5°C for all-the-year-round performance. At lower melting points than 27.5°C, declination in the cooling capacity of the storage unit was more profound as compared to the improvement in the heating capacity. Also, it was concluded that the melting point of the PCM that provided maximum cooling during summer season could be used for winter heating also but not vice versa.

  5. Effect of phase change material on the heat transfer rate of different building materials

    NASA Astrophysics Data System (ADS)

    Hasan, Mushfiq; Alam, Shahnur; Ahmed, Dewan Hasan

    2017-12-01

    Phase change material (PCM) is widely known as latent heat storage. A comprehensive study is carried out to investigate the effect of PCM on heat transfer rate of building materials. Paraffin is used as PCM along with different conventional building materials to investigate the heat transfer rate from the heated region to the cold region. PCM is placed along with the three different types of building materials like plaster which is well know building material in urban areas and wood and straw which are commonly used in rural areas for roofing as well as wall panel material and investigated the heat transfer rate. An experimental setup was constructed with number of rectangular shape aluminum detachable casing (as cavity) and placed side by side. Series of rectangular cavity filled with convent ional building materials and PCM and these were placed in between two chambers filled with water at different temperature. Building materials and PCM were placed in different cavities with different combinations and investigated the heat transfer rate. The results show that using the PCM along with other building materials can be used to maintain lower temperature at the inner wall and chamber of the cold region. Moreover, the placement or orientation of the building materials and PCM make significant contribution to heat transfer rate from the heated zone to the cold zone.

  6. 4. FIRST FLOOR INTERIOR, AMMONIA COMPRESSION DYNAMOS IN MACHINERY ROOM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. FIRST FLOOR INTERIOR, AMMONIA COMPRESSION DYNAMOS IN MACHINERY ROOM ALONG SOUTH SIDE OF WESTERN PORTION OF BUILDING, FROM EASTERN ENTRANCE TO MACHINERY ROOM, LOOKING WEST. - Oakland Naval Supply Center, Cold Storage Warehouse, South of C Street between First & Second Street, Oakland, Alameda County, CA

  7. Safety analysis report for packaging (onsite) multicanister overpack cask

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Edwards, W.S.

    1997-07-14

    This safety analysis report for packaging (SARP) documents the safety of shipments of irradiated fuel elements in the MUlticanister Overpack (MCO) and MCO Cask for a highway route controlled quantity, Type B fissile package. This SARP evaluates the package during transfers of (1) water-filled MCOs from the K Basins to the Cold Vacuum Drying Facility (CVDF) and (2) sealed and cold vacuum dried MCOs from the CVDF in the 100 K Area to the Canister Storage Building in the 200 East Area.

  8. Aquifer thermal energy storage. International symposium: Proceedings

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1995-05-01

    Aquifers have been used to store large quantities of thermal energy to supply process cooling, space cooling, space heating, and ventilation air preheating, and can be used with or without heat pumps. Aquifers are used as energy sinks and sources when supply and demand for energy do not coincide. Aquifer thermal energy storage may be used on a short-term or long-term basis; as the sole source of energy or as a partial storage; at a temperature useful for direct application or needing upgrade. The sources of energy used for aquifer storage are ambient air, usually cold winter air; waste ormore » by-product energy; and renewable energy such as solar. The present technical, financial and environmental status of ATES is promising. Numerous projects are operating and under development in several countries. These projects are listed and results from Canada and elsewhere are used to illustrate the present status of ATES. Technical obstacles have been addressed and have largely been overcome. Cold storage in aquifers can be seen as a standard design option in the near future as it presently is in some countries. The cost-effectiveness of aquifer thermal energy storage is based on the capital cost avoidance of conventional chilling equipment and energy savings. ATES is one of many developments in energy efficient building technology and its success depends on relating it to important building market and environmental trends. This paper attempts to provide guidance for the future implementation of ATES. Individual projects have been processed separately for entry onto the Department of Energy databases.« less

  9. Solar heating and hot water system installed at office building, One Solar Place, Dallas, Texas

    NASA Technical Reports Server (NTRS)

    1980-01-01

    A solar heating on cooling system is described which is designed to provide 87 percent of the space heating needs, 100 percent of the potable hot water needs and is sized for future absorption cooling. The collection subsystem consists of 28 solargenics, series 76, flat plate collectors with a total area of 1,596 square feet. The solar loop circulates an ethylene glyco water solution through the collectors into a hot water system exchanger. The water storage subsystem consists of a heat exchanger, two 2,300 gallon concrete hot water storage tanks with built in heat exchangers and a back-up electric boiler. The domestic hot water subsystem sends hot water to the 10,200 square feet floor area office building hot water water fixtures. The building cold water system provides make up to the solar loop, the heating loop, and the hot water concrete storage tanks. The design, construction, cost analysis, operation and maintenance of the solar system are described.

  10. ETR AND MTR COMPLEXES IN CONTEXT. CAMERA FACING NORTHERLY. FROM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ETR AND MTR COMPLEXES IN CONTEXT. CAMERA FACING NORTHERLY. FROM BOTTOM TO TOP: ETR COOLING TOWER, ELECTRICAL BUILDING AND LOW-BAY SECTION OF ETR BUILDING, HEAT EXCHANGER BUILDING (WITH U SHAPED YARD), COMPRESSOR BUILDING. MTR REACTOR SERVICES BUILDING IS ATTACHED TO SOUTH WALL OF MTR. WING A IS ATTACHED TO BALCONY FLOOR OF MTR. NEAR UPPER RIGHT CORNER OF VIEW IS MTR PROCESS WATER BUILDING. WING B IS AT FAR WEST END OF COMPLEX. NEAR MAIN GATE IS GAMMA FACILITY, WITH "COLD" BUILDINGS BEYOND: RAW WATER STORAGE TANKS, STEAM PLANT, MTR COOLING TOWER PUMP HOUSE AND COOLING TOWER. INL NEGATIVE NO. 56-4101. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  11. Criticality Safety Evaluation Report CSER-96-019 for Spent Nuclear Fuel (SNF) Processing and Storage Facilities Multi Canister Overpack (MCO)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    KESSLER, S.F.

    This criticality evaluation is for Spent N Reactor fuel unloaded from the existing canisters in both KE and KW Basins, and loaded into multiple canister overpack (MCO) containers with specially built baskets containing a maximum of either 54 Mark IV or 48 Mark IA fuel assemblies. The criticality evaluations include loading baskets into the cask-MCO, operation at the Cold Vacuum Drying Facility,a nd storage in the Canister Storage Building. Many conservatisms have been built into this analysis, the primary one being the selection of the K{sub eff} = 0.95 criticality safety limit. This revision incorporates the analyses for the sampling/weldmore » station in the Canister Storage Building and additional analysis of the MCO during the draining at CVDF. Additional discussion of the scrap basket model was added to show why the addition of copper divider plates was not included in the models.« less

  12. Management self assessment plan

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Debban, B.L.

    Duke Engineering and Services Hanford Inc., Spent Nuclear Fuel Project is responsible for the operation of fuel storage facilities. The SNF project mission includes the safe removal, processing and transportation of Spent Nuclear Fuel from 100 K Area fuel storage basins to a new Storage facility in the Hanford 200 East Area. Its mission is the modification of the 100 K area fuel storage facilities and the construction of two new facilities: the 100 K Area Cold Vacuum Drying Facility, and the 200 East Area Canister Storage Building. The management self assessment plan described in this document is scheduled tomore » begin in April of 1999 and be complete in May of 1999. The management self assessment plan describes line management preparations for declaring that line management is ready to commence operations.« less

  13. Cold air systems: Sleeping giant

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    MacCracken, C.D.

    1994-04-01

    This article describes how cold air systems help owners increase the profits from their buildings by reducing electric costs and improving indoor air quality through lower relative humidity levels. Cold air distribution involves energy savings, cost savings, space savings, greater comfort, cleaner air, thermal storage, tighter ducting, coil redesign, lower relative humidities, retrofitting, and improved indoor air quality (IAQ). It opens a door for architects, engineers, owners, builders, environmentalists, retrofitters, designers, occupants, and manufacturers. Three things have held up cold air's usage: multiple fan-powered boxes that ate up the energy savings of primary fans. Cold air room diffusers that providedmore » inadequate comfort. Condensation from ducts, boxes, and diffusers. Such problems have been largely eliminated through research and development by utilities and manufacturers. New cold air diffusers no longer need fan powered boxes. It has also been found that condensation is not a concern so long as the ducts are located in air conditioned space, such as drop ceilings or central risers, where relative humidity falls quickly during morning startup.« less

  14. Hen genetic strain and extended cold storage implications on physical egg quality from cage-free aviary housing system

    USDA-ARS?s Scientific Manuscript database

    In the US there is a sudden increase in the need for cage-free eggs in retail and food manufacturing sectors. As the US industry adapts existing and builds new cage-free housing structures, there is a need to understand the impact of cage-free systems and the corresponding management on egg quality...

  15. Evaporative cooling enhanced cold storage system

    DOEpatents

    Carr, Peter

    1991-01-01

    The invention provides an evaporatively enhanced cold storage system wherein a warm air stream is cooled and the cooled air stream is thereafter passed into contact with a cold storage unit. Moisture is added to the cooled air stream prior to or during contact of the cooled air stream with the cold storage unit to effect enhanced cooling of the cold storage unit due to evaporation of all or a portion of the added moisture. Preferably at least a portion of the added moisture comprises water condensed during the cooling of the warm air stream.

  16. Evaporative cooling enhanced cold storage system

    DOEpatents

    Carr, P.

    1991-10-15

    The invention provides an evaporatively enhanced cold storage system wherein a warm air stream is cooled and the cooled air stream is thereafter passed into contact with a cold storage unit. Moisture is added to the cooled air stream prior to or during contact of the cooled air stream with the cold storage unit to effect enhanced cooling of the cold storage unit due to evaporation of all or a portion of the added moisture. Preferably at least a portion of the added moisture comprises water condensed during the cooling of the warm air stream. 3 figures.

  17. Unsteady Heat Transfer Behavior of Reinforced Concrete Wall of Cold Storage

    NASA Astrophysics Data System (ADS)

    Nomura, Tomohiro; Murakami, Yuji; Uchikawa, Motoyuki

    The authors had already clarified that the heat transfer behaviors between internal and external insulated reinforced concrete wall of cold storage are different each others when inside and outside temperature of wall is flactuating. From that conclusion, we must consider the application method of wall insulation of cold storages in actual design. The theme of the paper is to get the analyzing method and unsteady heat transfer characteristics of concrete walls of cold storage during daily variation of outside temperature of walls, and to give the basis for efficient design and cost optimization of insulate wall of cold storage. The difference of unsteady heat transfer characteristics between internal and external insulate wall, when outside temperature of the wall follewed daily varation, was clarified in experiment and in situ measurement of practical cold storage. The analyzing method with two dimentional unsteady FEM was introduced. Using this method, it is possible to obtain the time variation of heat flux, which is important basic factor for practical design of cold storage, through the wall.

  18. Single bi-temperature thermal storage tank for application in solar thermal plant

    DOEpatents

    Litwin, Robert Zachary; Wait, David; Lancet, Robert T.

    2017-05-23

    Thermocline storage tanks for solar power systems are disclosed. A thermocline region is provided between hot and cold storage regions of a fluid within the storage tank cavity. One example storage tank includes spaced apart baffles fixed relative to the tank and arranged within the thermocline region to substantially physically separate the cavity into hot and cold storage regions. In another example, a flexible baffle separated the hot and cold storage regions and deflects as the thermocline region shifts to accommodate changing hot and cold volumes. In yet another example, a controller is configured to move a baffle within the thermocline region in response to flow rates from hot and cold pumps, which are used to pump the fluid.

  19. Cold storage of rat hepatocyte suspensions for one week in a customized cold storage solution--preservation of cell attachment and metabolism.

    PubMed

    Pless-Petig, Gesine; Singer, Bernhard B; Rauen, Ursula

    2012-01-01

    Primary hepatocytes are of great importance for basic research as well as cell transplantation. However, their stability, especially in suspension, is very low. This feature severely compromises storage and shipment. Based on previous studies with adherent cells, we here assessed cold storage injury in rat hepatocyte suspensions and aimed to find a cold storage solution that preserves viability, attachment ability and functionality of these cells. Rat hepatocyte suspensions were stored in cell culture medium, organ preservation solutions and modified TiProtec solutions at 4°C for one week. Viability and cell volume were determined by flow cytometry. Thereafter, cells were seeded and density and metabolic capacity (reductive metabolism, forskolin-induced glucose release, urea production) of adherent cells were assessed. Cold storage injury in hepatocyte suspensions became evident as cell death occurring during cold storage or rewarming or as loss of attachment ability. Cell death during cold storage was not dependent on cell swelling and was almost completely inhibited in the presence of glycine and L-alanine. Cell attachment could be greatly improved by use of chloride-poor solutions and addition of iron chelators. Using a chloride-poor, potassium-rich storage solution containing glycine, alanine and iron chelators, cultures with 75% of the density of control cultures and with practically normal cell metabolism could be obtained after one week of cold storage. In the solution presented here, cold storage injury of hepatocyte suspensions, differing from that of adherent hepatocytes, was effectively inhibited. The components which acted on the different injurious processes were identified.

  20. Development of a single-phase thermosiphon for cold collection and storage of radiative cooling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zhao, Dongliang; Martini, Christine Elizabeth; Jiang, Siyu

    A single-phase thermosiphon is developed for cold collection and storage of radiative cooling. Compared to the conventional nocturnal radiative cooling systems that use an electric pump to drive the heat transfer fluid, the proposed single-phase thermosiphon uses the buoyancy force to drive heat transfer fluid. This solution does not require electricity, therefore improving the net gain of the radiative cooling system. A single-phase thermosiphon was built, which consists of a flat panel, a cold collection tank, a water return tube, and a water distribution tank. Considering that outdoor radiative cooling flux is constantly changing (i.e. uncontrollable), an indoor testing facilitymore » was developed to provide a controllable cooling flux (comparable to a radiative cooling flux of 100 W/m2) for the evaluation of thermosiphon performance. The testing apparatus is a chilled aluminum flat plate that has a controlled air gap separation relative to the flat panel surface of the thermosiphon to emulate radiative cooling. With an average of 105 W/m2 cooling flux, the 18 liters of water in the thermosiphon was cooled to an average temperature of 12.5 degrees C from an initial temperature of 22.2 degrees C in 2 h, with a cold collection efficiency of 96.8%. The results obtained have demonstrated the feasibility of using a single-phase thermosiphon for cold collection and storage of radiative cooling. Additionally, the effects of the thermosiphon operation conditions, such as tilt angle of the flat panel, initial water temperature, and cooling energy flux, on the performance have been experimentally investigated. Modular design of the single-phase thermosiphon gives flexibility for its scalability. A radiative cooling system with multiple thermosiphon modules is expected to play an important role in cooling buildings and power plant condensers.« less

  1. Maximising platelet availability by delaying cold storage.

    PubMed

    Wood, B; Johnson, L; Hyland, R A; Marks, D C

    2018-04-06

    Cold-stored platelets may be an alternative to conventional room temperature (RT) storage. However, cold-stored platelets are cleared more rapidly from circulation, reducing their suitability for prophylactic transfusion. To minimise wastage, it may be beneficial to store platelets conventionally until near expiry (4 days) for prophylactic use, transferring them to refrigerated storage to facilitate an extended shelf life, reserving the platelets for the treatment of acute bleeding. Two ABO-matched buffy-coat-derived platelets (30% plasma/70% SSP+) were pooled and split to produce matched pairs (n = 8 pairs). One unit was stored at 2-6°C without agitation (day 1 postcollection; cold); the second unit was stored at 20-24°C with constant agitation until day 4 then stored at 2-6°C thereafter (delayed-cold). All units were tested for in vitro quality periodically over 21 days. During storage, cold and delayed-cold platelets maintained a similar platelet count. While pH and HSR were significantly higher in delayed-cold platelets, other metabolic markers, including lactate production and glucose consumption, did not differ significantly. Furthermore, surface expression of phosphatidylserine and CD62P, release of soluble CD62P and microparticles were not significantly different, suggesting similar activation profiles. Aggregation responses of delayed-cold platelets followed the same trend as cold platelets once transferred to cold storage, gradually declining over the storage period. The metabolic and activation profile of delayed-cold platelets was similar to cold-stored platelets. These data suggest that transferring platelets to refrigerated storage when near expiry may be a viable option for maximising platelet inventories. © 2018 International Society of Blood Transfusion.

  2. High brilliant thermal and cold moderator for the HBS neutron source project Jülich

    NASA Astrophysics Data System (ADS)

    Cronert, T.; Dabruck, J. P.; Doege, P. E.; Bessler, Y.; Klaus, M.; Hofmann, M.; Zakalek, P.; Rücker, U.; Lange, C.; Butzek, M.; Hansen, W.; Nabbi, R.; Brückel, T.

    2016-09-01

    The proposed High Brilliance Neutron Source (HBS), recognized within the Helmholtz Association of German Research Centres, will optimize the entire chain from particle source through particle accelerator, target, moderator, reflector, shielding, beam extraction, beam transport all the way to the detector, utilizing the nuclear Be(p,n) or Be(d,n) reaction in the lower MeV energy range. A D2O moderating reflector prototype (MRP) and a cold source were constructed and build according to MCNP parameter studies. The MRP was tested in a feasibility study at the TREFF instrument at MLZ (Garching). Cold beam extraction from the flux maximum within the moderator based on liquid para H2 and other cold moderators will be tested by energy spectroscopy via TOF-method. Different ratios of liquid ortho/para H2 will be fed to the cold moderator. The ratio will be controlled by feeding from reservoires of natural liquid H2 and a storage loop with an ortho/para converter and determined via online heat capacity measurement.

  3. Sample storage-induced changes in the quantity and quality of soil labile organic carbon

    PubMed Central

    Sun, Shou-Qin; Cai, Hui-Ying; Chang, Scott X.; Bhatti, Jagtar S.

    2015-01-01

    Effects of sample storage methods on the quantity and quality of labile soil organic carbon are not fully understood even though their effects on basic soil properties have been extensively studied. We studied the effects of air-drying and frozen storage on cold and hot water soluble organic carbon (WSOC). Cold- and hot-WSOC in air-dried and frozen-stored soils were linearly correlated with those in fresh soils, indicating that storage proportionally altered the extractability of soil organic carbon. Air-drying but not frozen storage increased the concentrations of cold-WSOC and carbohydrate in cold-WSOC, while both increased polyphenol concentrations. In contrast, only polyphenol concentration in hot-WSOC was increased by air-drying and frozen storage, suggesting that hot-WSOC was less affected by sample storage. The biodegradability of cold- but not hot-WSOC was increased by air-drying, while both air-drying and frozen storage increased humification index and changed specific UV absorbance of both cold- and hot-WSOC, indicating shifts in the quality of soil WSOC. Our results suggest that storage methods affect the quantity and quality of WSOC but not comparisons between samples, frozen storage is better than air-drying if samples have to be stored, and storage should be avoided whenever possible when studying the quantity and quality of both cold- and hot-WSOC. PMID:26617054

  4. 1. Cold Storage Warehouse, east facade. Northeast corner of the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Cold Storage Warehouse, east facade. Northeast corner of the north facade of the Ice Plant is visible on the left. Far left, the Creamery. - Curtis Wharf, Cold Storage Warehouse, O & Second Streets, Anacortes, Skagit County, WA

  5. Utilization of the organ care system as ex-vivo lung perfusion after cold storage transportation.

    PubMed

    Mohite, P N; Maunz, O; Popov, A-F; Zych, B; Patil, N P; Simon, A R

    2015-11-01

    The Organ Care System (OCS) allows perfusion and ventilation of the donor lungs under physiological conditions. Ongoing trials to compare preservation with OCS Lung with standard cold storage do not include donor lungs with suboptimal gas exchange and donor lungs treated with OCS following cold storage transportation. We present a case of a 48-yr-old man who received such lungs after cold storage transportation treated with ex-vivo lung perfusion utilizing OCS. © The Author(s) 2015.

  6. Hypothermic machine perfusion permits extended cold ischemia times with improved early graft function.

    PubMed

    Guy, Alison; McGrogan, Damian; Inston, Nicholas; Ready, Andrew

    2015-04-01

    The logistics of deceased-donor renal transplants are largely affected by cold ischemia time. However, to attain successful outcomes, other issues must be considered. Extending cold ischemia time to accommodate these issues would be valuable. We investigated the role of hypothermic machine perfusion to extend cold ischaemia time. Deceased-donor kidneys were allocated to a storage method, depending on predicted time to operation. Kidneys to be transplanted from 8:00 AM to 8:00 PM in the transplant room remained in static cold storage. If predicted operating time was out of hours, the kidney was transferred to hypothermic machine perfusion and transplanted at the earliest opportunity on the dedicated transplant list. There were 74 kidneys transplanted from hypothermic machine perfusion and 101 kidneys from static cold storage. Median cold ischemia time was 23.85 hours in the hypothermic machine perfusion group, compared with 13 hours in the static cold storage group (P ≤ .0001). There were 20 kidneys (27%) from hypothermic machine perfusion that had delayed graft function, compared with 47 kidneys (47%) in the static cold storage group (P = .012). There were no other significant differences in graft or postoperative complications. This study demonstrated that improved early graft outcomes can be achieved following longer cold ischemia time by using hypothermic machine perfusion rather than static cold storage. This effect is likely multifactorial including the inherent effects of hypothermic machine perfusion, improved recipient preparation, and possibly better perioperative conditions.

  7. Artificial Permafrost and the Application to the Low Temperature Storage for Foodstuffs

    NASA Astrophysics Data System (ADS)

    Ryokai, Kimitoshi; Fukuda, Masami

    In the cold regions like Hokkaido and Tohoku Districts, they have been advocating snow-overcoming, advantages of snow and effective utilization of cold climate. In fact, they have been positively trying to make use of snow and coldness as water resources, energy sources, structural materials and so on. One of energy utilization is for low temperature storage of foods. Since the potatoes have properties of adapting themselves to cold temperature when they are stored under cold environment, they have the tendency of growing in their sugar contents. As the results, all those foods which are stored under these cold environments will be the products of higher additional value. Here we will introduce the present situation of low temperature storage of foods by artificial permafrost, not only as the construction materials for cold storage house itself but also utilizing its own cold temperature.

  8. Valued Sustainable Services: Building Partnership Capacity Through Collaborating Approaches

    DTIC Science & Technology

    2013-07-01

    and national Security Policy, institute for national Strategic Studies , at the national defense university. he is also a distinguished research...for choosing a site for ValSServ initiation is the enthusiasm of the population—that is, the priority generally is to work with those who are...applied to domains to improve daily life in a ValSServ project area. Agriculture/Food. Power for cool or cold storage and on-site processing of

  9. 40 CFR 75.64 - Quarterly reports.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... placed in long-term cold storage (as defined in § 72.2 of this chapter), quarterly reports are not... the unit). For units placed into long-term cold storage during a reporting quarter, the exemption from... long-term cold storage. For any provisionally-certified monitoring system, § 75.20(a)(3) shall apply...

  10. 40 CFR 75.64 - Quarterly reports.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... placed in long-term cold storage (as defined in § 72.2 of this chapter), quarterly reports are not... the unit). For units placed into long-term cold storage during a reporting quarter, the exemption from... long-term cold storage. For any provisionally-certified monitoring system, § 75.20(a)(3) shall apply...

  11. 40 CFR 75.64 - Quarterly reports.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... placed in long-term cold storage (as defined in § 72.2 of this chapter), quarterly reports are not... the unit). For units placed into long-term cold storage during a reporting quarter, the exemption from... long-term cold storage. For any provisionally-certified monitoring system, § 75.20(a)(3) shall apply...

  12. 40 CFR 75.64 - Quarterly reports.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... placed in long-term cold storage (as defined in § 72.2 of this chapter), quarterly reports are not... the unit). For units placed into long-term cold storage during a reporting quarter, the exemption from... long-term cold storage. For any provisionally-certified monitoring system, § 75.20(a)(3) shall apply...

  13. Technology Application of Environmental Friendly Refrigeration (Green Refrigeration) on Cold Storage for Fishery Industry

    NASA Astrophysics Data System (ADS)

    Rasta, IM; Susila, IDM; Subagia, IWA

    2018-01-01

    The application of refrigeration technology to postharvest fishery products is an very important. Moreover, Indonesia is a tropical region with relatively high temperatures. Fish storage age can be prolonged with a decrease in temperature. Frozen fish can even be stored for several months. Fish freezing means preparing fish for storage in low-temperature cold storage. The working fluid used in cold storage to cool low-temperature chambers and throw heat into high-temperature environments is refrigerant. So far refrigerant used in cold storage is Hydrochloroflourocarbons (HCFC) that is R-22. Chlor is a gas that causes ODP (Ozone Depleting Potential), while Flour is a gas that causes GWP (Global Warming Potential). Government policy began in 2015 to implement Hydrochloroflourocarbons Phase-Out Management Plan. Hydrocarbon (HC) is an alternative substitute for R-22. HC-22 (propane ≥ 99.5%) has several advantages, among others: environmentally friendly, indicated by a zero ODP value, and GWP = 3 (negligible), thermophysical property and good heat transfer characteristics, vapor phase density Which is low, and good solubility with mineral lubricants. The use of HC-22 in cold storage is less than R-22. From the analysis results obtained, cold storage system using HC-22 has better performance and energy consumption is more efficient than the R-22.

  14. 7 CFR 1436.4 - Application for loans.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., except loans for renewable biomass storage facilities and cold storage facilities for fruits and... the administrative county office. (2) For loans for renewable biomass storage facilities and cold...

  15. 7 CFR 1436.4 - Application for loans.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., except loans for renewable biomass storage facilities and cold storage facilities for fruits and... the administrative county office. (2) For loans for renewable biomass storage facilities and cold...

  16. 7 CFR 1436.4 - Application for loans.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., except loans for renewable biomass storage facilities and cold storage facilities for fruits and... the administrative county office. (2) For loans for renewable biomass storage facilities and cold...

  17. 7 CFR 1436.4 - Application for loans.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., except loans for renewable biomass storage facilities and cold storage facilities for fruits and... the administrative county office. (2) For loans for renewable biomass storage facilities and cold...

  18. 7 CFR 1436.4 - Application for loans.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., except loans for renewable biomass storage facilities and cold storage facilities for fruits and... the administrative county office. (2) For loans for renewable biomass storage facilities and cold...

  19. Low oxygen treatment prior to cold storage decreases the incidence of bitter pit in 'Golden Reinders' apples.

    PubMed

    Val, Jesús; Fernández, Victoria; López, Paola; Peiró, Jose María; Blanco, Alvaro

    2010-02-01

    The effect of subjecting 'Golden Reinders' apples to a low O(2) pre-treatment (LOT; 1-2% O(2)) was evaluated as a strategy to decrease the rate of bitter pit (BP) incidence after standard cold storage (ST). Immediately after harvest, apples were stored for 10 days at 20 degrees C under low O(2). Thereafter, apples were cold-stored (0-4 degrees C) for 4 months and changes were monitored in terms of BP incidence, fruit quality traits and mineral element concentrations. After 4 months cold storage, LOT apples presented a 2.6-fold decrease in the rate of BP incidence (14%) versus the values obtained for standard cold-stored fruits (37% BP incidence). LOT increased flesh firmness, total soluble solids and titratable acidity as compared to the quality traits determined for cold-stored fruits. Lower cortex Ca and Mg concentrations as compared to ST apples were determined in association with LOT, 2 months after cold storage. Application of a LOT prior to cold storage may be a promising strategy to reduce the incidence of BP and preserve fruit quality, which should be further investigated.

  20. Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions.

    PubMed

    Johnson, Lacey; Tan, Shereen; Wood, Ben; Davis, April; Marks, Denese C

    2016-07-01

    Alternatives to room temperature storage of platelets (PLTs) may be beneficial to extend the limited shelf life and support transfusion logistics in rural and military areas. The aim of this study was to assess the morphologic, metabolic, and functional aspects of PLTs stored at room temperature or in refrigerated conditions or cryopreserved. A three-arm pool-and-split study was carried out using buffy coat-derived PLTs stored in 30% plasma/70% SSP+. The three matched treatment arms were room temperature stored (20-24°C), cold-stored (2-6°C), and cryopreserved (-80°C with dimethyl sulfoxide). Liquid-stored PLTs were tested over a 21-day period, while cryopreserved PLTs were examined immediately after thawing and after 6 and 24 hours of storage at room temperature. Cold-stored and cryopreserved PLTs underwent a significant shape change, although the cryopreserved PLTs appeared to recover from this during subsequent storage. Glycolytic metabolism was reduced in cold-stored PLTs, but accelerated in cryopreserved PLTs, while oxidative phosphorylation was negatively affected by both storage conditions. PLT aggregation was potentiated by cold storage and diminished by cryopreservation in comparison to room temperature-stored PLTs. Cold storage and cryopreservation resulted in faster clot formation (R-time; thromboelastography), which was associated with an increase in microparticles. Cold storage and cryopreservation of PLTs led to morphologic and metabolic changes. However, storage under these conditions appears to maintain or even enhance certain aspects of in vitro PLT function. © 2016 AABB.

  1. Ultrasonic monitoring of Iberian fat crystallization during cold storage

    NASA Astrophysics Data System (ADS)

    Corona, E.; García-Pérez, J. V.; Santacatalina, J. V.; Peña, R.; Benedito, J.

    2012-12-01

    The aim of this work was to evaluate the use of ultrasonic measurements to characterize the crystallization process and to assess the textural changes of Iberian fat and Iberian ham during cold storage. The ultrasonic velocity was measured in two types of Iberian fats (Montanera and Cebo) during cold storage (0, 2, 5, 7 and 10 °C) and in vacuum packaged Iberian ham stored at 6°C for 120 days. The fatty acid profile, thermal behaviour and textural properties of fat were determined. The ultrasonic velocity and textural measurements showed a two step increase during cold storage, which was related with the separate crystallization of two fractions of triglycerides. It was observed that the harder the fat, the higher the ultrasonic velocity. Likewise, Cebo fat resulted harder than Montanera due to a higher content of saturated triglycerides. The ultrasonic velocity in Iberian ham showed an average increase of 55 m/s after 120 days of cold storage due to fat crystallization. Thus, non-destructive ultrasonic technique could be a reliable method to follow the crystallization of fats and to monitor the changes in the textural properties of Iberian ham during cold storage.

  2. Mitochondrial Impairment as a Key Factor for the Lack of Attachment after Cold Storage of Hepatocyte Suspensions

    PubMed Central

    Pless-Petig, Gesine; Walter, Björn; Bienholz, Anja

    2018-01-01

    Isolated primary hepatocytes, which are widely used for pharmacological and clinical purposes, usually undergo certain periods of cold storage in suspension during processing. While adherent hepatocytes were shown previously to suffer iron-dependent cell death during cold (4 °C) storage and early rewarming, we previously found little iron-dependent hepatocyte death in suspension but severely decreased attachment ability unless iron chelators were added. Here, we focus on the role of mitochondrial impairment in this nonattachment of hepatocyte suspensions. Rat hepatocyte suspensions were stored in a chloride-poor, glycine-containing cold storage solution with and without iron chelators at 4 °C. After 1 wk of cold storage in the basic cold storage solution, cell viability in suspension was unchanged, while cell attachment was decreased by >80%. In the stored cells, a loss of mitochondrial membrane potential (MMP), a decrease in adenosine triphosphate (ATP) content (2 ± 2 nmol/106 cells after cold storage, 5 ± 3 nmol/106 cells after rewarming vs. control 29 ± 6 nmol/106 cells), and a decrease in oxygen consumption (101 ± 59 pmol sec−1 per 106 cells after rewarming vs. control 232 ± 83 pmol sec−1 per 106 cells) were observed. Addition of iron chelators to the cold storage solution increased cell attachment to 53% ± 20% and protected against loss of MMP, and cells were able to partially regenerate ATP during rewarming (15 ± 10 nmol/106 cells). Increased attachment could also be achieved by addition of the inhibitor combination of mitochondrial permeability transition, trifluoperazine + fructose. Attached hepatocytes displayed normal MMP and mitochondrial morphology. Additional experiments with freshly isolated hepatocytes confirmed that impaired energy production—as elicited by an inhibitor of the respiratory chain, antimycin A—can decrease cell attachment without decreasing viability. Taken together, these results suggest that mitochondrial impairment with subsequent energy deficiency is a key factor for the lack of attachment of cold-stored hepatocyte suspensions. PMID:29390882

  3. Mitochondrial Impairment as a Key Factor for the Lack of Attachment after Cold Storage of Hepatocyte Suspensions.

    PubMed

    Pless-Petig, Gesine; Walter, Björn; Bienholz, Anja; Rauen, Ursula

    2017-12-01

    Isolated primary hepatocytes, which are widely used for pharmacological and clinical purposes, usually undergo certain periods of cold storage in suspension during processing. While adherent hepatocytes were shown previously to suffer iron-dependent cell death during cold (4 °C) storage and early rewarming, we previously found little iron-dependent hepatocyte death in suspension but severely decreased attachment ability unless iron chelators were added. Here, we focus on the role of mitochondrial impairment in this nonattachment of hepatocyte suspensions. Rat hepatocyte suspensions were stored in a chloride-poor, glycine-containing cold storage solution with and without iron chelators at 4 °C. After 1 wk of cold storage in the basic cold storage solution, cell viability in suspension was unchanged, while cell attachment was decreased by >80%. In the stored cells, a loss of mitochondrial membrane potential (MMP), a decrease in adenosine triphosphate (ATP) content (2 ± 2 nmol/10 6 cells after cold storage, 5 ± 3 nmol/10 6 cells after rewarming vs. control 29 ± 6 nmol/10 6 cells), and a decrease in oxygen consumption (101 ± 59 pmol sec -1 per 10 6 cells after rewarming vs. control 232 ± 83 pmol sec -1 per 10 6 cells) were observed. Addition of iron chelators to the cold storage solution increased cell attachment to 53% ± 20% and protected against loss of MMP, and cells were able to partially regenerate ATP during rewarming (15 ± 10 nmol/10 6 cells). Increased attachment could also be achieved by addition of the inhibitor combination of mitochondrial permeability transition, trifluoperazine + fructose. Attached hepatocytes displayed normal MMP and mitochondrial morphology. Additional experiments with freshly isolated hepatocytes confirmed that impaired energy production-as elicited by an inhibitor of the respiratory chain, antimycin A-can decrease cell attachment without decreasing viability. Taken together, these results suggest that mitochondrial impairment with subsequent energy deficiency is a key factor for the lack of attachment of cold-stored hepatocyte suspensions.

  4. A novel combination technique of cold crystalloid perfusion but not cold storage facilitates transplantation of canine hearts donated after circulatory death.

    PubMed

    Rosenfeldt, Franklin; Ou, Ruchong; Salamonsen, Robert; Marasco, Silvana; Zimmet, Adam; Byrne, Joshua; Cosic, Filip; Saxena, Pankaj; Esmore, Donald

    2016-11-01

    Donation after circulatory death (DCD) represents a potential new source of hearts to increase the donor pool. We showed previously that DCD hearts in Greyhound dogs could be resuscitated and preserved by continuous cold crystalloid perfusion but not by cold static storage and could demonstrate excellent contractile and metabolic function on an in vitro system. In the current study, we demonstrate that resuscitated DCD hearts are transplantable. Donor Greyhound dogs (n = 12) were divided into perfusion (n = 8) and cold static storage (n = 4) groups. General anesthesia was induced and ventilation ceased for 30 minutes to achieve circulatory death. Donor cardiectomy was performed, and for 4 hours the heart was preserved by controlled reperfusion, followed by continuous cold perfusion with an oxygenated crystalloid perfusate or by static cold storage, after which orthotopic heart transplantation was performed. Recovery was assessed over 4 hours by hemodynamic monitoring. During cold perfusion, hearts showed continuous oxygen consumption and low lactate levels, indicating aerobic metabolism. The 8 dogs in the perfusion group were weaned off bypass, and 4 hours after bypass produced cardiac output of 4.73 ± 0.51 liters/min, left ventricular power of 7.63 ± 1.32 J/s, right ventricular power of 1.40 ± 0.43 J/s, and left ventricular fractional area shortening of 39.1% ± 5.2%, all comparable to pre-transplant values. In the cold storage group, 3 of 4 animals could not be weaned from cardiopulmonary bypass, and the fourth exhibited low-level function. Cold crystalloid perfusion, but not cold static storage, can resuscitate and preserve the DCD donor heart in a canine model of heart transplantation, thus rendering it transplantable. Controlled reperfusion and cold crystalloid perfusion have potential for clinical application in DCD transplantation. Copyright © 2016. Published by Elsevier Inc.

  5. The sensitivity of human mesenchymal stem cells to vibration and cold storage conditions representative of cold transportation

    PubMed Central

    Nikolaev, N. I.; Liu, Y.; Hussein, H.; Williams, D. J.

    2012-01-01

    In the current study, the mechanical and hypothermic damage induced by vibration and cold storage on human mesenchymal stem cells (hMSCs) stored at 2–8°C was quantified by measuring the total cell number and cell viability after exposure to vibration at 50 Hz (peak acceleration 140 m s−2 and peak displacement 1.4 mm), 25 Hz (peak acceleration 140 m s−2, peak displacement 5.7 mm), 10 Hz (peak acceleration 20 m s−2, peak displacement 5.1 mm) and cold storage for several durations. To quantify the viability of the cells, in addition to the trypan blue exclusion method, the combination of annexin V-FITC and propidium iodide was applied to understand the mode of cell death. Cell granularity and a panel of cell surface markers for stemness, including CD29, CD44, CD105 and CD166, were also evaluated for each condition. It was found that hMSCs were sensitive to vibration at 25 Hz, with moderate effects at 50 Hz and no effects at 10 Hz. Vibration at 25 Hz also increased CD29 and CD44 expression. The study further showed that cold storage alone caused a decrease in cell viability, especially after 48 h, and also increased CD29 and CD44 and attenuated CD105 expressions. Cell death would most likely be the consequence of membrane rupture, owing to necrosis induced by cold storage. The sensitivity of cells to different vibrations within the mechanical system is due to a combined effect of displacement and acceleration, and hMSCs with a longer cold storage duration were more susceptible to vibration damage, indicating a coupling between the effects of vibration and cold storage. PMID:22628214

  6. Possibility of the market expansion of large capacity optical cold archive

    NASA Astrophysics Data System (ADS)

    Matsumoto, Ikuo; Sakata, Emiko

    2017-08-01

    The field, IoT and Big data, which is activated by the revolution of ICT, has caused rapid increase of distribution data of various business application. As a result, data with low access frequency has been rapidly increasing into a huge scale that human has never experienced before. This data with low access frequency is called "cold data", and the storage for cold data is called "cold storage". In this situation, the specifications of storage including access frequency, response speed and cost is determined by the application's request.

  7. Cold Heat Storage Characteristics of O/W-type Latent Heat Emulsion Including Continuum Phase of Water Treated with a Freezing Point Depression

    NASA Astrophysics Data System (ADS)

    Inaba, Hideo; Morita, Shin-Ichi

    This paper deals with flow and cold heat storage characteristics of the oil (tetradecane, C14H30, freezing point 278.9 K, Latent heat 229 kJ/kg)/water emulsion as a latent heat storage material having a low melting point. The test emulsion includes a water-urea solution as a continuum phase. The freezing point depression of the continuum phase permits enhancement of the heat transfer rate of the emulison, due to the large temperature difference between the latent heat storage material and water-urea solution. The velocity of emulsion flow and the inlet temperature of coolant in a coiled double tube heat exchanger are chosen as the experimental parameters. The pressure drop, the heat transfer coefficient of the emulsion in the coiled tube are measured in the temperture region over solid and liquid phase of the latent heat storage material. The finishing time of the cold heat storage is defined experimentally in the range of sensible and latent heat storage. It is clarified that the flow behavior of the emulsion as a non-Newtonian fluid has an important role in cold heat storage. The useful nondimentional correlation equations for the additional pressure loss coefficient, the heat transfer coefficient and the finishing time of the cold heat storage are derived in terms of Dean number and heat capacity ratio.

  8. [Effects of low temperature on dormancy breaking and growth after planting in bulbs of Tulipa edulis].

    PubMed

    Yang, Ying; Zhu, Zai-Biao; Guo, Qiao-Sheng; Miao, Yuan-Yuan; Ma, Hong-Liang; Yang, Xiao-Hua

    2015-01-01

    The effect of low temperature storage on dormancy breaking, sprouting and growth after planting of Tulipa edulis was studied. The results showed that starch content and activity of amylases significantly decreased during 10 weeks of cold storage, soluble protein content raised at first then decreased, and the peak appeared at the 6th week. However, total soluble sugar content which in- creased slowly at first than rose sharply and reducing sugar content increased during the storage duration. The bulbs with cold storage treatment rooted in the 6th week, which was about 2 weeks earlier than room temperature storage, but there were less new roots in the late period of storage. After stored at a low temperature, bud lengths were longer than that with room temperature treatment. Cold storage treatment could promote earlier emergence, shorten germination time, prolong growth period and improve the yield of bulb, but rarely affect the emergence rate. It was not beneficial to flowering and fruiting. The results indicated that 6-8 weeks of cold storage was deemed to be the key period of dormancy breaking preliminary.

  9. MitoQ blunts mitochondrial and renal damage during cold preservation of porcine kidneys.

    PubMed

    Parajuli, Nirmala; Campbell, Lia H; Marine, Akira; Brockbank, Kelvin G M; Macmillan-Crow, Lee Ann

    2012-01-01

    Cold preservation has greatly facilitated the use of cadaveric kidneys for transplantation but damage occurs during the preservation episode. It is well established that oxidant production increases during cold renal preservation and mitochondria are a key target for injury. Our laboratory has demonstrated that cold storage of renal cells and rat kidneys leads to increased mitochondrial superoxide levels and mitochondrial electron transport chain damage, and that addition of Mitoquinone (MitoQ) to the preservation solutions blunted this injury. In order to better translate animal studies, the inclusion of large animal models is necessary to develop safe preclinical protocols. Therefore, we tested the hypothesis that addition of MitoQ to cold storage solution preserves mitochondrial function by decreasing oxidative stress, leading to less renal tubular damage during cold preservation of porcine kidneys employing a standard criteria donor model. Results showed that cold storage significantly induced oxidative stress (nitrotyrosine), renal tubular damage, and cell death. Using High Resolution Respirometry and fresh porcine kidney biopsies to assess mitochondrial function we showed that MitoQ significantly improved complex II/III respiration of the electron transport chain following 24 hours of cold storage. In addition, MitoQ blunted oxidative stress, renal tubular damage, and cell death after 48 hours. These results suggested that MitoQ decreased oxidative stress, tubular damage and cell death by improving mitochondrial function during cold storage. Therefore this compound should be considered as an integral part of organ preservation solution prior to transplantation.

  10. MitoQ Blunts Mitochondrial and Renal Damage during Cold Preservation of Porcine Kidneys

    PubMed Central

    Parajuli, Nirmala; Campbell, Lia H.; Marine, Akira; Brockbank, Kelvin G. M.; MacMillan-Crow, Lee Ann

    2012-01-01

    Cold preservation has greatly facilitated the use of cadaveric kidneys for transplantation but damage occurs during the preservation episode. It is well established that oxidant production increases during cold renal preservation and mitochondria are a key target for injury. Our laboratory has demonstrated that cold storage of renal cells and rat kidneys leads to increased mitochondrial superoxide levels and mitochondrial electron transport chain damage, and that addition of Mitoquinone (MitoQ) to the preservation solutions blunted this injury. In order to better translate animal studies, the inclusion of large animal models is necessary to develop safe preclinical protocols. Therefore, we tested the hypothesis that addition of MitoQ to cold storage solution preserves mitochondrial function by decreasing oxidative stress, leading to less renal tubular damage during cold preservation of porcine kidneys employing a standard criteria donor model. Results showed that cold storage significantly induced oxidative stress (nitrotyrosine), renal tubular damage, and cell death. Using High Resolution Respirometry and fresh porcine kidney biopsies to assess mitochondrial function we showed that MitoQ significantly improved complex II/III respiration of the electron transport chain following 24 hours of cold storage. In addition, MitoQ blunted oxidative stress, renal tubular damage, and cell death after 48 hours. These results suggested that MitoQ decreased oxidative stress, tubular damage and cell death by improving mitochondrial function during cold storage. Therefore this compound should be considered as an integral part of organ preservation solution prior to transplantation. PMID:23139796

  11. Solar hot water system installed at Day's Inn Motel, Savannah, Georgia

    NASA Technical Reports Server (NTRS)

    1980-01-01

    The Solar System was designed to provide 50 percent of the total Domestic Hot Water (DHW) demand. Liquid Flat Plate Collectors (900 square feet) are used for the collector subsystem. The collector subsystem is closed loop, using 50 percent Ethylene Glycol solution antifreeze for freeze protection. The 1,000 gallon fiber glass storage tank contains two heat exchangers. One of the heat exchangers heats the storage tank with the collector solar energy. The other heat exchanger preheats the cold supply water as it passes through on the way to the Domestic Hot Water (DHW) tank heaters. Electrical energy supplements the solar energy for the DHW. The Collector Mounting System utilizes guy wires to structurally tie the collector array to the building.

  12. Neutron Imaging at the Oak Ridge National Laboratory: Application to Biological Research

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bilheux, Hassina Z; Cekanova, Maria; Bilheux, Jean-Christophe

    2014-01-01

    The Oak Ridge National Laboratory Neutron Sciences Directorate (NScD) has recently installed a neutron imaging beamline at the High Flux Isotope Reactor (HFIR) cold guide hall. The CG-1D beamline supports a broad range of user research spanning from engineering to material research, energy storage, additive manufacturing, vehicle technologies, archaeology, biology, and plant physiology. The beamline performance (spatial resolution, field of view, etc.) and its utilization for biological research are presented. The NScD is also considering a proposal to build the VENUS imaging beamline (beam port 10) at the Spallation Neutron Source (SNS). Unlike CG-1D which provides cold neutrons, VENUS willmore » offer a broad range of neutron wavelengths, from epithermal to cold, and enhanced contrast mechanisms. This new capability will also enable the imaging of thicker biological samples than is currently available at CG-1D. A brief overview of the VENUS capability for biological research is discussed.« less

  13. Ultrasonic characterization of pork fat crystallization during cold storage.

    PubMed

    Corona, Edith; García-Pérez, José V; Santacatalina, Juan V; Ventanas, Sonia; Benedito, José

    2014-05-01

    In this work, the feasibility of using ultrasonic velocity measurements for characterizing and differentiating the crystallization pattern in 2 pork backfats (Montanera and Cebo Iberian fats) during cold storage (0 °C, 2 °C, 5 °C, 7 °C, and 10 °C) was evaluated. The fatty acid profile, thermal behavior, and textural properties (hardness) of fat were also determined. Both fats became harder during cold storage (average hardness increase for both fats, 11.5 N, 8 N, and 1.8 N at 0, °C 2 °C, and 5 °C , respectively), showing a 2-step pattern related with the separate crystallization of the different existing triacylglycerols, which was well described using a modified Avrami equation (explained variance > 99%). Due to a greater content of saturated triacylglycerols, Cebo fat (45.1%) was harder than Montanera (41.8%). The ultrasonic velocity followed a similar 2-step pattern to hardness during cold storage, being found an average increase for both fats of 184, 161, and 150 m/s at 0 °C 2 °C, and 5 °C, respectively. Thus, ultrasonic measurements were useful both to characterize the textural changes taking place during cold storage and to differentiate between fats with different composition. The cold storage of dry-cured meat products during their distribution and retail sale exert an important effect on their textural properties and consumers' acceptance due to the crystallization of the fat fraction, which is greatly influenced by the type of fat. In this work, a nondestructive ultrasonic technique was used to identify the textural changes provoked by the crystallization during cold storage, and to differentiate between fats, which could be used for quality control purposes. © 2014 Institute of Food Technologists®

  14. Cold storage effects on egg hatch in laboratory-reared Culicoides variipennis sonorensis (Diptera: Ceratopogonidae).

    PubMed

    Hunt, G J; Tabachnick, W J

    1995-09-01

    The effects of cold storage (5 degrees C) on the hatching rates of laboratory-reared Culicoides variipennis sonorensis eggs were examined. Mortality increased with storage time. Average maximum embryo survivorship for 4 trials was 55.0 +/- 4.2 (+/- SEM) days. Alternating daily cycles of high and then low mean hatching rates occurred and possibly were due to location differences in temperature within the temperature-controlled rearing system. During cold storage at 5 degrees C, C. v. sonorensis eggs may be kept for ca. 28 days with an anticipated hatching rate of about 50%.

  15. Effect of cooking and cold storage on biologically active antibiotic residues in meat.

    PubMed Central

    O'Brien, J. J.; Campbell, N.; Conaghan, T.

    1981-01-01

    An investigation was undertaken to see if cooking or cold storage would destroy or decrease the level of biologically active antibiotic in tissues from animals given therapeutic doses of antibiotic on three occasions prior to slaughter. The effects of cooking and cold storage on the biological activity of the residues of ampicillin, chloramphenicol, oxytetracycline, streptomycin and sulphadimidine were varied; in some instances the effects were minimal, in others nil. PMID:7310129

  16. Ethylene-dependent regulation of an α-L-arabinofuranosidase is associated to firmness loss in 'Gala' apples under long term cold storage.

    PubMed

    Storch, Tatiane Timm; Finatto, Taciane; Pegoraro, Camila; Dal Cero, Joceani; Laurens, François; Rombaldi, Cesar Valmor; Quecini, Vera; Girardi, César Luís

    2015-09-01

    Fruit texture changes impair the quality of apples submitted to long term storage, especially under cold. The changes are due to cell wall modifications during ripening and senescence and are associated to ethylene. We have investigated the activity of α-l-arabinofuranosidase, a glycosyl hydrolase acting on the side chains of pectin in the cell wall and middle lamella. The transcription of arabinofuranosidase coding sequences 1 and 3 was investigated in plant organs and in response to ethylene, employing hormone application and 1-methylcyclopropene. The transcription of arabinofuranosidase genes is not restricted to fruits, although upregulated by ripening and ethylene. Transcripts of the genes were detected under cold storage up to 180 days. Similarly, arabinofuranosidase activity increased with rising levels of ethylene and under cold storage. Levels of arabinofuranosidase3 transcripts were higher than those of arabinofuranosidase1, suggesting that the first is an important contributor to enzyme activity and texture changes during cold storage. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Cold-Chain Adaptability During Introduction of Inactivated Polio Vaccine in Bangladesh, 2015.

    PubMed

    Billah, Mallick M; Zaman, K; Estivariz, Concepcion F; Snider, Cynthia J; Anand, Abhijeet; Hampton, Lee M; Bari, Tajul I A; Russell, Kevin L; Chai, Shua J

    2017-07-01

    Introduction of inactivated polio vaccine creates challenges in maintaining the cold chain for vaccine storage and distribution. We evaluated the cold chain in 23 health facilities and 36 outreach vaccination sessions in 8 districts and cities of Bangladesh, using purposive sampling during August-October 2015. We interviewed immunization and cold-chain staff, assessed equipment, and recorded temperatures during vaccine storage and transportation. All health facilities had functioning refrigerators, and 96% had freezers. Temperature monitors were observed in all refrigerators and freezers but in only 14 of 66 vaccine transporters (21%). Recorders detected temperatures >8°C for >60 minutes in 5 of 23 refrigerators (22%), 3 of 6 cold boxes (50%) transporting vaccines from national to subnational depots, and 8 of 48 vaccine carriers (17%) used in outreach vaccination sites. Temperatures <2°C were detected in 4 of 19 cold boxes (21%) transporting vaccine from subnational depots to health facilities and 14 of 48 vaccine carriers (29%). Bangladesh has substantial cold-chain storage and transportation capacity after inactivated polio vaccine introduction, but temperature fluctuations during vaccine transport could cause vaccine potency loss that could go undetected. Bangladesh and other countries should strive to ensure consistent and sufficient cold-chain storage and monitor the cold chain during vaccine transportation at all levels. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America.

  18. Effect of Cold Storage on Biological Traits of Psix saccharicola (Hymenoptera: Platygastridae), an Egg Parasitoid of Acrosternum arabicum (Hemiptera: Pentatomidae).

    PubMed

    Forouzan, Farzane; Jalali, Mohammad Amin; Ziaaddini, Mahdi; Hashemi Rad, Hamid

    2018-05-28

    Psix saccharicola (Mani) (Hymenoptera: Platygastridae) is a solitary egg parasitoid of the pistachio green stink bug, Acrosternum arabicum (Wagner) (Hemiptera: Pentatomidae), which is one of the most important pests of pistachio in Iran. Augmentation of P. saccharicola field populations using mass-reared individuals may provide an alternative to conventional pesticide use for pistachio green stink bug control. Cold storage is an important component of mass-rearing protocols for optimum timing of host egg parasitization and potentially extended storage of P. saccharicola pupae prior to adult emergence. The impact of cold storage on A. arabicum eggs for various time intervals at 4.0°C was investigated. Results indicated that host eggs stored at 4.0°C for up to 60 d could be exploited by P. sacchricola, whereas no offspring were produced when eggs were stored for 120 d. The emergence rates of the F1 and F2 generations declined with increased host egg storage time. Both sex ratio and survival rate of the F2 generation decreased as the refrigeration time of host eggs increased. The impact of cold storage on P. saccharicola pupae was evaluated. Reared pupae of P. saccharicola were held for 1 wk at three temperatures and compared with a control (27 ± 1°C). Psix saccharicola pupae were tolerant to cold storage at 8 and 12°C. Cold storage adversely affected mean adult emergence at 4°C, which decreased following low temperature exposure. Furthermore, mean percentage survivorship was unaffected by storage at low temperatures in the F1 generation, but was reduced at 4°C. The sex ratio of the F1 generation became more male-biased when held at lower storage temperatures. The highest female proportion was observed at 12°C.

  19. Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing

    PubMed Central

    Correia, Cláudia; Koshkin, Alexey; Carido, Madalena; Espinha, Nuno; Šarić, Tomo; Lima, Pedro A.; Alves, Paula M.

    2016-01-01

    To fully explore the potential of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs), efficient methods for storage and shipment of these cells are required. Here, we evaluated the feasibility to cold store monolayers and aggregates of functional CMs obtained from different PSC lines using a fully defined clinical-compatible preservation formulation and investigated the time frame that hPSC-CMs could be subjected to hypothermic storage. We showed that two-dimensional (2D) monolayers of hPSC-CMs can be efficiently stored at 4°C for 3 days without compromising cell viability. However, cell viability decreased when the cold storage interval was extended to 7 days. We demonstrated that hPSC-CMs are more resistant to prolonged hypothermic storage-induced cell injury in three-dimensional aggregates than in 2D monolayers, showing high cell recoveries (>70%) after 7 days of storage. Importantly, hPSC-CMs maintained their typical (ultra)structure, gene and protein expression profile, electrophysiological profiles, and drug responsiveness. Significance The applicability of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) in the clinic/industry is highly dependent on the development of efficient methods for worldwide shipment of these cells. This study established effective clinically compatible strategies for cold (4°C) storage of hPSC-CMs cultured as two-dimensional (2D) monolayers and three-dimensional (3D) aggregates. Cell recovery of 2D monolayers of hPSC-CMs was found to be dependent on the time of storage, and 3D cell aggregates were more resistant to prolonged cold storage than 2D monolayers. Of note, it was demonstrated that 7 days of cold storage did not affect hPSC-CM ultrastructure, phenotype, or function. This study provides important insights into the cold preservation of PSC-CMs that could be valuable in improving global commercial distribution of hPSC-CMs. PMID:27025693

  20. Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing.

    PubMed

    Correia, Cláudia; Koshkin, Alexey; Carido, Madalena; Espinha, Nuno; Šarić, Tomo; Lima, Pedro A; Serra, Margarida; Alves, Paula M

    2016-05-01

    To fully explore the potential of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs), efficient methods for storage and shipment of these cells are required. Here, we evaluated the feasibility to cold store monolayers and aggregates of functional CMs obtained from different PSC lines using a fully defined clinical-compatible preservation formulation and investigated the time frame that hPSC-CMs could be subjected to hypothermic storage. We showed that two-dimensional (2D) monolayers of hPSC-CMs can be efficiently stored at 4°C for 3 days without compromising cell viability. However, cell viability decreased when the cold storage interval was extended to 7 days. We demonstrated that hPSC-CMs are more resistant to prolonged hypothermic storage-induced cell injury in three-dimensional aggregates than in 2D monolayers, showing high cell recoveries (>70%) after 7 days of storage. Importantly, hPSC-CMs maintained their typical (ultra)structure, gene and protein expression profile, electrophysiological profiles, and drug responsiveness. The applicability of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) in the clinic/industry is highly dependent on the development of efficient methods for worldwide shipment of these cells. This study established effective clinically compatible strategies for cold (4°C) storage of hPSC-CMs cultured as two-dimensional (2D) monolayers and three-dimensional (3D) aggregates. Cell recovery of 2D monolayers of hPSC-CMs was found to be dependent on the time of storage, and 3D cell aggregates were more resistant to prolonged cold storage than 2D monolayers. Of note, it was demonstrated that 7 days of cold storage did not affect hPSC-CM ultrastructure, phenotype, or function. This study provides important insights into the cold preservation of PSC-CMs that could be valuable in improving global commercial distribution of hPSC-CMs. ©AlphaMed Press.

  1. INTERIOR OF COLD STORAGE ROOM, SHOWING MOVABLE HANGING RACKS. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR OF COLD STORAGE ROOM, SHOWING MOVABLE HANGING RACKS. - Naval Air Station Barbers Point, Aircraft Storehouse, Between Midway & Card Streets at Enterprise Avenue intersection, Ewa, Honolulu County, HI

  2. Thermal energy storage with geothermal triplet for space heating and cooling

    NASA Astrophysics Data System (ADS)

    Bloemendal, Martin; Hartog, Niels

    2017-04-01

    Many governmental organizations and private companies have set high targets in avoiding CO2 emissions and reducing energy (Kamp, 2015; Ministry-of-Economic-affairs, 2016). ATES systems use groundwater wells to overcome the discrepancy in time between the availability of heat (during summer) and the demand for heat (during winter). Aquifer Thermal Energy Storage is an increasingly popular technique; currently over 2000 ATES systems are operational in the Netherlands (Graaf et al., 2016). High temperature ATES may help to improve performance of these conventional ATES systems. ATES systems use heat pumps to get the stored heat to the required temperature for heating of around 40-50°C and to produce the cold water for cooling in summer. These heat pumps need quite a lot of power to run; on average an ATES system produces 3-4 times less CO2 emission compared to conventional. Over 60% of those emission are accounted for by the heat pump (Dekker, 2016). This heat pump power consumption can be reduced by utilizing other sources of sustainable heat and cooling capacity for storage in the subsurface. At such operating temperatures the required storage temperatures do no longer match the return temperatures in the building systems. Therefore additional components and an additional well are required to increase the groundwater temperature in summer (e.g. solar collectors) and decrease it in winter (e.g. dry coolers). To prevent "pollution" of the warm and cold well return water from the building can be stored in a third well until weather conditions are suitable for producing the required storage temperature. Simulations and an economical evaluation show great potential for this type of aquifer thermal energy storage; economic performance is better than normal ATES while the emissions are reduce by a factor ten. At larger temperature differences, also the volume of groundwater required to pump around is much less, which causes an additional energy saving. Research now focusses on energy balance and energy loss in the subsurface, well design requirements, working/operational conditions of each well, as well as building system components like the influence of weather conditions on performance of system components. At EGU we like to present and discuss the results of this research. references • Dekker, L.d., 2016. Bepalende factoren voor goed functionerende WKO, kennisplatform bodemenergie. • Graaf, A.d., Heijer, R., Postma, S., 2016. Evaluatie Wijzigingsbesluit bodemenergiesystemen. Buro 38 in commision of ministry of Intrastructure and environment, Cothen. • Kamp, H., 2015. Warmtevisie, ministry of economic affairs, Den Haag. • Ministry-of-Economic-affairs, 2016. Energieagenda, Naar een CO₂-arme energievoorziening. Ministry of Economic affairs, Den Haag.

  3. N2 Gas Flushing Alleviates the Loss of Bacterial Diversity and Inhibits Psychrotrophic Pseudomonas during the Cold Storage of Bovine Raw Milk.

    PubMed

    Gschwendtner, Silvia; Alatossava, Tapani; Kublik, Susanne; Fuka, Mirna Mrkonjić; Schloter, Michael; Munsch-Alatossava, Patricia

    2016-01-01

    The quality and safety of raw milk still remains a worldwide challenge. Culture-dependent methods indicated that the continuous N2 gas-flushing of raw milk reduced the bacterial growth during cold storage by up to four orders of magnitude, compared to cold storage alone. This study investigated the influence of N2 gas-flushing on bacterial diversity in bovine raw-milk samples, that were either cold stored at 6°C or additionally flushed with pure N2 for up to one week. Next-generation sequencing (NGS) of the V1-V2 hypervariable regions of 16S rRNA genes, derived from amplified cDNA, which was obtained from RNA directly isolated from raw-milk samples, was performed. The reads, which were clustered into 2448 operational taxonomic units (OTUs), were phylogenetically classified. Our data revealed a drastic reduction in the diversity of OTUs in raw milk during cold storage at 6°C at 97% similarity level; but, the N2-flushing treatment alleviated this reduction and substantially limited the loss of bacterial diversity during the same cold-storage period. Compared to cold-stored milk, the initial raw-milk samples contained less Proteobacteria (mainly Pseudomonadaceae, Moraxellaceae and Enterobacteriaceae) but more Firmicutes (mainly Ruminococcaceaea, Lachnospiraceae and Oscillospiraceaea) and Bacteroidetes (mainly Bacteroidales). Significant differences between cold-stored and additionally N2-flushed milk were mainly related to higher levels of Pseudomononadaceae (including the genera Pseudomonas and Acinetobacter) in cold-stored milk samples; furthermore, rare taxa were better preserved by the N2 gas flushing compared to the cold storage alone. No major changes in bacterial composition with time were found regarding the distribution of the major 9 OTUs, that dominated the Pseudomonas genus in N2-flushed or non-flushed milk samples, other than an intriguing predominance of bacteria related to P. veronii. Overall, this study established that neither bacteria causing milk spoilage nor any well-known human pathogen or anaerobe benefited from the N2 gas flushing even though the N2-flushed and non-flushed cold-stored milk differed in bacterial counts by up to 104-fold.

  4. N2 Gas Flushing Alleviates the Loss of Bacterial Diversity and Inhibits Psychrotrophic Pseudomonas during the Cold Storage of Bovine Raw Milk

    PubMed Central

    Kublik, Susanne; Fuka, Mirna Mrkonjić; Schloter, Michael; Munsch-Alatossava, Patricia

    2016-01-01

    The quality and safety of raw milk still remains a worldwide challenge. Culture-dependent methods indicated that the continuous N2 gas-flushing of raw milk reduced the bacterial growth during cold storage by up to four orders of magnitude, compared to cold storage alone. This study investigated the influence of N2 gas-flushing on bacterial diversity in bovine raw-milk samples, that were either cold stored at 6°C or additionally flushed with pure N2 for up to one week. Next-generation sequencing (NGS) of the V1-V2 hypervariable regions of 16S rRNA genes, derived from amplified cDNA, which was obtained from RNA directly isolated from raw-milk samples, was performed. The reads, which were clustered into 2448 operational taxonomic units (OTUs), were phylogenetically classified. Our data revealed a drastic reduction in the diversity of OTUs in raw milk during cold storage at 6°C at 97% similarity level; but, the N2-flushing treatment alleviated this reduction and substantially limited the loss of bacterial diversity during the same cold-storage period. Compared to cold-stored milk, the initial raw-milk samples contained less Proteobacteria (mainly Pseudomonadaceae, Moraxellaceae and Enterobacteriaceae) but more Firmicutes (mainly Ruminococcaceaea, Lachnospiraceae and Oscillospiraceaea) and Bacteroidetes (mainly Bacteroidales). Significant differences between cold-stored and additionally N2-flushed milk were mainly related to higher levels of Pseudomononadaceae (including the genera Pseudomonas and Acinetobacter) in cold-stored milk samples; furthermore, rare taxa were better preserved by the N2 gas flushing compared to the cold storage alone. No major changes in bacterial composition with time were found regarding the distribution of the major 9 OTUs, that dominated the Pseudomonas genus in N2-flushed or non-flushed milk samples, other than an intriguing predominance of bacteria related to P. veronii. Overall, this study established that neither bacteria causing milk spoilage nor any well-known human pathogen or anaerobe benefited from the N2 gas flushing even though the N2-flushed and non-flushed cold-stored milk differed in bacterial counts by up to 104-fold. PMID:26730711

  5. An improved red blood cell additive solution maintains 2,3-diphosphoglycerate and adenosine triphosphate levels by an enhancing effect on phosphofructokinase activity during cold storage.

    PubMed

    Burger, Patrick; Korsten, Herbert; De Korte, Dirk; Rombout, Eva; Van Bruggen, Robin; Verhoeven, Arthur J

    2010-11-01

    Current additive solutions (ASs) for red blood cells (RBCs) do not maintain constant 2,3-diphosphoglycerate (DPG) and adenosine triphosphate (ATP) levels during cold storage. We have previously shown that with a new AS called phosphate-adenine-glucose-guanosine-gluconate-mannitol (PAGGGM), both 2,3-DPG and ATP could be maintained throughout storage for 35 days. In this study, the mechanism underlying the effect of PAGGGM on RBC storage was studied in more detail. By using double-erythrocytapheresis units (leukoreduced), a direct comparison could be made between the current AS saline-adenine-glucose-mannitol (SAGM) and the experimental solution PAGGGM. During cold storage, several in vitro characteristics were analyzed. In agreement with our previous findings with single RBCs, PAGGGM maintained 2,3-DPG and ATP levels for 35 days of cold storage. Furthermore, glucose consumption and lactate production were higher in PAGGGM units during the first 21 days of cold storage. Fructose-1,6-diphophate and dihydroxyacetone phosphate levels were also increased during the first 21 days of storage in PAGGGM units. These results indicate that it is likely that phosphofructokinase (PFK) activity is enhanced in PAGGGM units relative to SAGM units. After 21 days, PFK activity also decreases in PAGGGM units, but sufficient metabolic reserve in these units prevents depletion of 2,3-DPG and ATP. © 2010 American Association of Blood Banks.

  6. Transcriptomic Profiling during the Post-Harvest of Heat-Treated Dixiland Prunus persica Fruits: Common and Distinct Response to Heat and Cold

    PubMed Central

    Lauxmann, Martin A.; Brun, Bianca; Borsani, Julia; Bustamante, Claudia A.; Budde, Claudio O.; Lara, María V.; Drincovich, María F.

    2012-01-01

    Cold storage is extensively used to slow the rapid deterioration of peach (Prunus persica L. Batsch) fruit after harvest. However, peach fruit subjected to long periods of cold storage develop chilling injury (CI) symptoms. Post-harvest heat treatment (HT) of peach fruit prior to cold storage is effective in reducing some CI symptoms, maintaining fruit quality, preventing softening and controlling post-harvest diseases. To identify the molecular changes induced by HT, which may be associated to CI protection, the differential transcriptome of peach fruit subjected to HT was characterized by the differential display technique. A total of 127 differentially expressed unigenes (DEUs), with a presence-absence pattern, were identified comparing peach fruit ripening at 20°C with those exposed to a 39°C-HT for 3 days. The 127 DEUs were divided into four expression profile clusters, among which the heat-induced (47%) and heat-repressed (36%) groups resulted the most represented, including genes with unknown function, or involved in protein modification, transcription or RNA metabolism. Considering the CI-protection induced by HT, 23-heat-responsive genes were selected and analyzed during and after short-term cold storage of peach fruit. More than 90% of the genes selected resulted modified by cold, from which nearly 60% followed the same and nearly 40% opposite response to heat and cold. Moreover, by using available Arabidopsis microarray data, it was found that nearly 70% of the peach-heat responsive genes also respond to cold in Arabidopsis, either following the same trend or showing an opposite response. Overall, the high number of common responsive genes to heat and cold identified in the present work indicates that HT of peach fruit after harvest induces a cold response involving complex cellular processes; identifying genes that are involved in the better preparation of peach fruit for cold-storage and unraveling the basis for the CI protection induced by HT. PMID:23236430

  7. Energy Performance and Optimal Control of Air-conditioned Buildings Integrated with Phase Change Materials

    NASA Astrophysics Data System (ADS)

    Zhu, Na

    This thesis presents an overview of the previous research work on dynamic characteristics and energy performance of buildings due to the integration of PCMs. The research work on dynamic characteristics and energy performance of buildings using PCMs both with and without air-conditioning is reviewed. Since the particular interest in using PCMs for free cooling and peak load shifting, specific research efforts on both subjects are reviewed separately. A simplified physical dynamic model of building structures integrated with SSPCM (shaped-stabilized phase change material) is developed and validated in this study. The simplified physical model represents the wall by 3 resistances and 2 capacitances and the PCM layer by 4 resistances and 2 capacitances respectively while the key issue is the parameter identification of the model. This thesis also presents the studies on the thermodynamic characteristics of buildings enhanced by PCM and on the investigation of the impacts of PCM on the building cooling load and peak cooling demand at different climates and seasons as well as the optimal operation and control strategies to reduce the energy consumption and energy cost by reducing the air-conditioning energy consumption and peak load. An office building floor with typical variable air volume (VAV) air-conditioning system is used and simulated as the reference building in the comparison study. The envelopes of the studied building are further enhanced by integrating the PCM layers. The building system is tested in two selected cities of typical climates in China including Hong Kong and Beijing. The cold charge and discharge processes, the operation and control strategies of night ventilation and the air temperature set-point reset strategy for minimizing the energy consumption and electricity cost are studied. This thesis presents the simulation test platform, the test results on the cold storage and discharge processes, the air-conditioning energy consumption and demand reduction potentials in typical air-conditioning seasons in typical China cites as well as the impacts of operation and control strategies.

  8. Improved platelet survival after cold storage by prevention of glycoprotein Ibα clustering in lipid rafts

    PubMed Central

    Gitz, Eelo; Koekman, Cornelis A; van den Heuvel, Dave J.; Deckmyn, Hans; Akkerman, Jan W.; Gerritsen, Hans C.; Urbanus, Rolf T.

    2012-01-01

    Background Storing platelets for transfusion at room temperature increases the risk of microbial infection and decreases platelet functionality, leading to out-date discard rates of up to 20%. Cold storage may be a better alternative, but this treatment leads to rapid platelet clearance after transfusion, initiated by changes in glycoprotein Ibα, the receptor for von Willebrand factor. Design and Methods: We examined the change in glycoprotein Ibα distribution using Förster resonance energy transfer by time-gated fluorescence lifetime imaging microscopy. Results Cold storage induced deglycosylation of glycoprotein Ibα ectodomain, exposing N-acetyl-Dglucosamine residues, which sequestered with GM1 gangliosides in lipid rafts. Raft-associated glycoprotein Ibα formed clusters upon binding of 14-3-3ζ adaptor proteins to its cytoplasmic tail, a process accompanied by mitochondrial injury and phosphatidyl serine exposure. Cold storage left glycoprotein Ibα surface expression unchanged and although glycoprotein V decreased, the fall did not affect glycoprotein Ibα clustering. Prevention of glycoprotein Ibα clustering by blockade of deglycosylation and 14-3-3ζ translocation increased the survival of cold-stored platelets to above the levels of platelets stored at room temperature without compromising hemostatic functions. Conclusions We conclude that glycoprotein Ibα translocates to lipid rafts upon cold-induced deglycosylation and forms clusters by associating with 14-3-3ζ. Interference with these steps provides a means to enable cold storage of platelet concentrates in the near future. PMID:22733027

  9. WEAPONS STORAGE AREA. FROM RIGHT TO LEFT, ABOVEGROUND STORAGE MAGAZINE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEAPONS STORAGE AREA. FROM RIGHT TO LEFT, ABOVEGROUND STORAGE MAGAZINE (BUILDING 3568), SPARES INERT STORAGE BUILDING (BUILDING 3570), MISSILE ASSEMBLY SHOP (BUILDING 3578) AND SEGREGATED MAGAZINE STORAGE BUILDING (BUILDING 3572). VIEW TO NORTHWEST - Plattsburgh Air Force Base, U.S. Route 9, Plattsburgh, Clinton County, NY

  10. Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage.

    PubMed

    Rašković, Brankica; Popović, Milica; Ostojić, Sanja; Anđelković, Boban; Tešević, Vele; Polović, Natalija

    2015-01-01

    Papain is a cysteine protease with wide substrate specificity and many applications. Despite its widespread applications, cold stability of papain has never been studied. Here, we used differential spectroscopy to monitor thermal denaturation process. Papain was the most stabile from 45 °C to 60 °C with ΔG°321 of 13.9±0.3 kJ/mol and Tm value of 84±1 °C. After cold storage, papain lost parts of its native secondary structures elements which gave an increase of 40% of intermolecular β-sheet content (band maximum detected at frequency of 1621 cm(-1) in Fourier transform infrared (FT-IR) spectrum) indicating the presence of secondary structures necessary for aggregation. The presence of protein aggregates after cold storage was also proven by analytical size exclusion chromatography. After six freeze-thaw cycles around 75% of starting enzyme activity of papain was lost due to cold denaturation and aggregation of unfolded protein. Autoproteolysis of papain did not cause significant loss of the protein activity. Upon the cold storage, papain underwent structural rearrangements and aggregation that correspond to other cold denatured proteins, rather than autoproteolysis which could have the commercial importance for the growing polypeptide based industry. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Continuous ozone concentrations during cold storage to control postharvest gray mold in grapes, 2011

    USDA-ARS?s Scientific Manuscript database

    Gray mold, caused by B. cinerea, causes severe losses since it spreads easily among berries during cold storage. Currently, it is controlled by fumigation with SO2 or SO2 emitting sheets within boxes. Alternative methods, such as storage in ozone atmospheres, are needed because SO2 is banned in orga...

  12. Effect of cold storage on total phenolics content, antioxidant activity and vitamin C level of selected potato clones.

    PubMed

    Külen, Oktay; Stushnoff, Cecil; Holm, David G

    2013-08-15

    Twelve Colorado-grown specialty potato clones were evaluated for total phenolic content, antioxidant activity and ascorbic acid content at harvest and after 2, 4, 6 and 7 months cold storage at 4 °C. Potato clones were categorized as pigmented ('CO97226-2R/R', 'CO99364-3R/R', 'CO97215-2P/P', 'CO97216-3P/P', 'CO97227-2P/P', 'CO97222-1R/R', 'Purple Majesty', 'Mountain Rose' and 'All Blue'), yellow ('Yukon Gold') and white fleshed ('Russet Nugget', 'Russet Burbank'). Folin-Ciocalteu reagent was used to estimate total phenolic content, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS(•+) ) and 2,2-diphenyl-1-picrylhydrazyl (DPPH(•) ) radical scavenging assays were used to estimate antioxidant capacity. Pigmented potato genotypes had significantly higher total phenolic content and antioxidant activity at all data points than yellow- and white-fleshed cultivars. Vitamin C content was higher in 'Yukon Gold' than in the other clones. The highest level of vitamin C in all clones was at harvest and after 2 months in cold storage. Vitamin C content in all potato clones dropped rapidly with longer intervals of cold storage. Although total phenolic content and antioxidant activity fluctuated during cold storage, after 7 months of cold storage their levels were slightly higher than at harvest. Total phenolic content was better correlated with Trolox equivalent antioxidant capacity (TEAC)/ABTS(•+) than the TEAC/DPPH(•) radical scavenging assay. Pigmented potato clones had significantly higher total phenolic content and antioxidant activity, while the yellow-fleshed potato cultivar 'Yukon Gold' had significantly higher vitamin C content. Vitamin C content decreased in all potato clones during cold storage, while total phenolics increased in pigmented clones. © 2013 Society of Chemical Industry.

  13. Cold storage of 'Manzanilla de Sevilla' and 'Manzanilla Cacereña' mill olives from super-high density orchards.

    PubMed

    Morales-Sillero, Ana; Pérez, Ana G; Casanova, Laura; García, José M

    2017-12-15

    The suitability of the cold storage (2°C) of fruit to maintain the quality of 'Manzanilla de Sevilla' and 'Manzanilla Cacereña' intended for virgin olive oil extraction was investigated. This temperature was effective in keeping the best commercial category of oil quality in both manually harvested olives and in mechanically harvested 'Manzanilla Cacereña' fruits for 11days. Mechanical harvesting induced significant decreases in oxidative stability and in the main phenolic compounds contents in the oils during cold storage and, only initially, in the total volatiles, regardless of the cultivar considered. However, the contents of volatile esters, associated to fruity flavor, were always higher in the oils from mechanically harvested fruits. 'Manzanilla de Sevilla' oils exhibited higher total volatiles during fruit cold storage, regardless of the harvesting system used. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Guidelines for maintaining and managing the vaccine cold chain.

    PubMed

    2003-10-24

    In February 2002, the Advisory Committee on Immunization Practices (ACIP) and American Academy of Family Physicians (AAFP) released their revised General Recommendations on Immunization, which included recommendations on the storage and handling of immunobiologics. Because of increased concern over the potential for errors with the vaccine cold chain (i.e., maintaining proper vaccine temperatures during storage and handling to preserve potency), this notice advises vaccine providers of the importance of proper cold chain management practices. This report describes proper storage units and storage temperatures, outlines appropriate temperature-monitoring practices, and recommends steps for evaluating a temperature-monitoring program. The success of efforts against vaccine-preventable diseases is attributable in part to proper storage and handling of vaccines. Exposure of vaccines to temperatures outside the recommended ranges can affect potency adversely, thereby reducing protection from vaccine-preventable diseases. Good practices to maintain proper vaccine storage and handling can ensure that the full benefit of immunization is realized.

  15. Research on Energy-saving Shape Design of High School Library Building in Cold Region

    NASA Astrophysics Data System (ADS)

    Hui, Zhao; Weishuang, Xie; Zirui, Tong

    2017-11-01

    Considering climatic characteristics in cold region, existing high school libraries in Changchun are researched according to investigation of real conditions of these library buildings. Mathematical analysis and CAD methods are used to summarize the relation between building shape and building energy saving of high school library. Strategies are put forward for sustainable development of high school library building in cold region, providing reliable design basis for construction of high school libraries in Changchun.

  16. Cold storage to overcome dormancy affects the carbon balance of azalea.

    PubMed

    Christiaens, A; De Keyser, E; Lootens, P; Pauwels, E; Roldan-Ruiz, I; De Riek, J; Gobin, B; Van Labeke, M-C

    2014-01-01

    Flower bud dormancy in azalea (Rhododendron simsii) is broken by artificial cold treatment and this will have its consequences on carbon reserves and photosynthesis. The effect of cold storage at 7 °C on carbohydrate and starch content in leaves and flower buds of an early ('Nordlicht') and semi-early ('M. Marie) flowering cultivar was quantified. Carbon loss due to respiration was lowest for 'M. Marie'. Photosynthetic measurements on 'Nordlicht' showed that photosynthesis 3 days after cold treatment (plants ready to flower) was improved compared to before cold treatment (plants with dormant flower buds).

  17. Tree age, fruit size and storage conditions affect levels of ascorbic acid, total phenolic concentrations and total antioxidant activity of 'Kinnow' mandarin juice.

    PubMed

    Khalid, Samina; Malik, Aman U; Khan, Ahmad S; Shahid, Muhammad; Shafique, Muhammad

    2016-03-15

    Bioactive compounds (ascorbic acid, total phenolics and total antioxidants) are important constituents of citrus fruit juice; however, information with regard to their concentrations and changes in relation to tree age and storage conditions is limited. 'Kinnow' (Citrus nobilis Lour × Citrus deliciosa Tenora) mandarin juice from fruit of three tree ages (6, 18 and 35 years old) and fruit sizes (large, medium and small) were examined for their bioactive compounds during 7 days under ambient storage conditions (20 ± 2 °C and 60-65% relative humidity (RH)) and during 60 days under cold storage (4 ± 1 °C and 75-80% RH) conditions. Under ambient conditions, a reduction in total phenolic concentrations (TPC) and in total antioxidant activity (TAA) was found for the juice from all tree ages and fruit sizes. Overall, fruit from 18-year-old trees had higher mean TPC (95.86 µg mL(-1) ) and TAA (93.68 mg L(-1) ), as compared to 6 and 35-year-old trees. Likewise, in cold storage, TAA decreased in all fruit size groups from 18 and 35-year-old trees. In all tree age and fruit size groups, TPC decreased initially during 15 days of cold storage and then increased gradually with increase in storage duration. Ascorbic acid concentrations showed an increasing trend in all fruit size groups from 35-year-old trees. Overall, during cold storage, fruit from 18-year-old trees maintained higher mean ascorbic acid (33.05 mg 100 mL(-1) ) concentrations, whereas fruit from 6-year-old trees had higher TAA (153.1 mg L(-1) ) and TPC (115.1 µg mL(-1) ). Large-sized fruit had higher ascorbic acid (32.08 mg 100 mL(-1) ) concentrations and TAA (157.5 mg L(-1) ). Fruit from 18-year-old trees maintained higher TPC and TAA under ambient storage conditions, whereas fruit from 6-year-old trees maintained higher TPC and TAA during cold storage. Small-sized fruit had higher TPC after ambient temperature storage, whereas large fruit size showed higher ascorbic acid concentrations and TAA after cold storage. © 2015 Society of Chemical Industry.

  18. Prior frozen storage enhances the effect of edible coatings against Listeria monocytogenes on cold-smoked salmon during subsequent refrigerated storage.

    PubMed

    Ye, M; Neetoo, H; Chen, H

    2011-10-01

    Listeria monocytogenes is a major safety concern for ready-to-eat foods. The overall objective of this study was to investigate whether prior frozen storage could enhance the efficacy of edible coatings against L. monocytogenes on cold-smoked salmon during subsequent refrigerated storage. A formulation consisting of sodium lactate (SL, 1·2-2·4%) and sodium diacetate (SD, 0·125-0·25%) or 2·5% Opti.Form (a commercial formulation of SL and SD) was incorporated into each of five edible coatings: alginate, κ-carrageenan, pectin, gelatin and starch. The coatings were applied onto the surface of cold-smoked salmon slices inoculated with L. monocytogenes at a level of 500 CFU cm⁻². In the first phase, the slices were first frozen at -18°C for 6 days and stored at 22°C for 6 days. Alginate, gelatin and starch appeared to be the most effective carriers. In the second phase, cold-smoked salmon slices were inoculated with L. monocytogenes, coated with alginate, gelatin or starch with or without the antimicrobials and stored frozen at -18°C for 12 months. Every 2 months, samples were removed from the freezer and kept at 4°C for 30 days. Prior frozen storage at -18°C substantially enhanced the antilisterial efficacy of the edible coatings with or without antimicrobials during the subsequent refrigerated storage. Plain coatings with ≥ 2 months frozen storage and antimicrobial edible coatings represent an effective intervention to inhibit the growth of L. monocytogenes on cold-smoked salmon. This study demonstrates the effectiveness of the conjunct application of frozen storage and edible coatings to control the growth of L. monocytogenes to enhance the microbiological safety of cold-smoked salmon. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.

  19. Arachidonic acid depletion extends survival of cold-stored platelets by interfering with the [glycoprotein Ibα – 14-3-3ζ] association

    PubMed Central

    van der Wal, Dianne E.; Gitz, Eelo; Du, Vivian X.; Lo, Kimberly S.L.; Koekman, Cornelis A.; Versteeg, Sabine; Akkerman, Jan Willem N.

    2012-01-01

    Background Cold storage of platelets reduces bacterial growth and preserves their hemostatic properties better than current procedures do. However, storage at 0°C induces [14-3-3ζ-glycoprotein Ibα] association, 14-3-3ζ release from phospho-Bad, Bad activation and apoptosis. Design and Methods We investigated whether arachidonic acid, which also binds 14-3-3ζ, contributes to coldinduced apoptosis. Results Cold storage activated P38-mitogen-activated protein kinase and released arachidonic acid, which accumulated due to cold inactivation of cyclooxygenase-1/thromboxane synthase. Accumulated arachidonic acid released 14-3-3ζ from phospho-Bad and decreased the mitochondrial membrane potential, which are steps in the induction of apoptosis. Addition of arachidonic acid did the same and its depletion made platelets resistant to cold-induced apoptosis. Incubation with biotin-arachidonic acid revealed formation of an [arachidonic acid-14-3-3ζ-glycoprotein Ibα] complex. Indomethacin promoted complex formation by accumulating arachidonic acid and released 14-3-3ζ from cyclo-oxygenase-1. Arachidonic acid depletion prevented the cold-induced reduction of platelet survival in mice. Conclusions We conclude that cold storage induced apoptosis through an [arachidonic acid-14-3-3ζ-glycoprotein Ibα] complex, which released 14-3-3ζ from Bad in an arachidonic acid-dependent manner. Although arachidonic acid depletion reduced agonist-induced thromboxane A2 formation and aggregation, arachidonic acid repletion restored these functions, opening ways to reduce apoptosis during storage without compromising hemostatic functions post-transfusion. PMID:22371179

  20. Under EPA Settlement, Chicopee, Mass. Cold Storage Warehouse Company Improves Public Protections

    EPA Pesticide Factsheets

    A Chicopee, Mass., company that operates a cold storage warehouse is spending more than half a million dollars, primarily on public safety enhancements, to resolve claims it violated the federal Clean Air Act's chemical release prevention requirements...

  1. Comparative transcriptome and metabolome provides new insights into the regulatory mechanisms of accelerated senescence in litchi fruit after cold storage.

    PubMed

    Yun, Ze; Qu, Hongxia; Wang, Hui; Zhu, Feng; Zhang, Zhengke; Duan, Xuewu; Yang, Bao; Cheng, Yunjiang; Jiang, Yueming

    2016-01-14

    Litchi is a non-climacteric subtropical fruit of high commercial value. The shelf life of litchi fruit under ambient conditions (AC) is approximately 4-6 days. Post-harvest cold storage prolongs the life of litchi fruit for up to 30 days with few changes in pericarp browning and total soluble solids. However, the shelf life of litchi fruits at ambient temperatures after pre-cold storage (PCS) is only 1-2 days. To better understand the mechanisms involved in the rapid fruit senescence induced by pre-cold storage, a transcriptome of litchi pericarp was constructed to assemble the reference genes, followed by comparative transcriptomic and metabolomic analyses. Results suggested that the senescence of harvested litchi fruit was likely to be an oxidative process initiated by ABA, including oxidation of lipids, polyphenols and anthocyanins. After cold storage, PCS fruit exhibited energy deficiency, and respiratory burst was elicited through aerobic and anaerobic respiration, which was regulated specifically by an up-regulated calcium signal, G-protein-coupled receptor signalling pathway and small GTPase-mediated signal transduction. The respiratory burst was largely associated with increased production of reactive oxygen species, up-regulated peroxidase activity and initiation of the lipoxygenase pathway, which were closely related to the accelerated senescence of PCS fruit.

  2. Comparative transcriptome and metabolome provides new insights into the regulatory mechanisms of accelerated senescence in litchi fruit after cold storage

    PubMed Central

    Yun, Ze; Qu, Hongxia; Wang, Hui; Zhu, Feng; Zhang, Zhengke; Duan, Xuewu; Yang, Bao; Cheng, Yunjiang; Jiang, Yueming

    2016-01-01

    Litchi is a non-climacteric subtropical fruit of high commercial value. The shelf life of litchi fruit under ambient conditions (AC) is approximately 4–6 days. Post-harvest cold storage prolongs the life of litchi fruit for up to 30 days with few changes in pericarp browning and total soluble solids. However, the shelf life of litchi fruits at ambient temperatures after pre-cold storage (PCS) is only 1–2 days. To better understand the mechanisms involved in the rapid fruit senescence induced by pre-cold storage, a transcriptome of litchi pericarp was constructed to assemble the reference genes, followed by comparative transcriptomic and metabolomic analyses. Results suggested that the senescence of harvested litchi fruit was likely to be an oxidative process initiated by ABA, including oxidation of lipids, polyphenols and anthocyanins. After cold storage, PCS fruit exhibited energy deficiency, and respiratory burst was elicited through aerobic and anaerobic respiration, which was regulated specifically by an up-regulated calcium signal, G-protein-coupled receptor signalling pathway and small GTPase-mediated signal transduction. The respiratory burst was largely associated with increased production of reactive oxygen species, up-regulated peroxidase activity and initiation of the lipoxygenase pathway, which were closely related to the accelerated senescence of PCS fruit. PMID:26763309

  3. The impact of cold storage and ethylene on volatile ester production and aroma perception in 'Hort16A' kiwifruit.

    PubMed

    Günther, Catrin S; Marsh, Ken B; Winz, Robert A; Harker, Roger F; Wohlers, Mark W; White, Anne; Goddard, Matthew R

    2015-02-15

    Fruit esters are regarded as key volatiles for fruit aroma. In this study, the effects of cold storage on volatile ester levels of 'Hort16A' (Actinidia chinensis Planch. var chinensis) kiwifruit were examined and the changes in aroma perception investigated. Cold storage (1.5°C) for two or four months of fruit matched for firmness and soluble solids concentration resulted in a significant reduction in aroma-related esters such as methyl/ethyl propanoate, methyl/ethyl butanoate and methyl/ethyl hexanoate. Levels of these esters, however, were restored by ethylene treatment (100ppm, 24h) before ripening. A sensory panel found that "tropical" and "fruit candy" aroma was stronger and "green" odour notes less intensively perceived in kiwifruit which were ethylene-treated after cold storage compared to untreated fruit. The key findings presented in this study may lead to further work on the ethylene pathway, and innovative storage and marketing solutions for current and novel fruit cultivars. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Effect of cobalt-60 gamma rays on the storage behaviour of garlic bulbs at room temperature and in cold storage

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Habibunnisa; Mathur, P.B.; Bano, Z.

    1971-11-01

    Effect of cobalt-60 gamma rays at a dose-rate of 6 krad on the storage behavior of garlic bulbs packaged individually and in lots of eight in perforated polyethylene bags of 200 gauge was investigated at room temperature (75 to 90 deg F) and cold temperature (32 to 35 deg F) under relative humidity 85 to 90%. Irradiation was immediately followed by an increase in the rate of respiration in the garlic bulbs followed by a decrease in the rate of respiration towards the later part of the storage period. At room temperature, sprouting was inhibited to a considerable extent, whilemore » in cold storage after a storage period of 9 months sprouting was completely prevented. The percentage sprouting was more in large size garlic bulbs than in small sized ones. For extension of storage life, packaging singly in polyethylene bags, selection of small sized garlic bulbs, storage at 32 to 35 deg F and irradiation with 6 krad of cobalt 60 gamma rays are recommended. (INIS)« less

  5. Chilling Stress Upregulates α-Linolenic Acid-Oxidation Pathway and Induces Volatiles of C6 and C9 Aldehydes in Mango Fruit.

    PubMed

    Sivankalyani, Velu; Maoz, Itay; Feygenberg, Oleg; Maurer, Dalia; Alkan, Noam

    2017-01-25

    Mango-fruit storage period and shelf life are prolonged by cold storage. However, chilling temperature induces physiological and molecular changes, compromising fruit quality. In our previous transcriptomic study of mango fruit, cold storage at suboptimal temperature (5 °C) activated the α-linolenic acid metabolic pathway. To evaluate changes in fruit quality during chilling, we analyzed mango "Keitt" fruit peel volatiles. GC-MS analysis revealed significant modulations in fruit volatiles during storage at suboptimal temperature. Fewer changes were seen in response to the time of storage. The mango volatiles related to aroma, such as δ-3-carene, (Z)-β-ocimene, and terpinolene, were downregulated during the storage at suboptimal temperature. In contrast, C 6 and C 9 aldehydes and alcohols-α-linolenic acid derivatives 1-hexanal, (Z)-3-hexenal, (Z)-3-hexenol, (E)-2-hexenal, and nonanal-were elevated during suboptimal-temperature storage, before chilling-injury symptoms appeared. Detection of those molecules before chilling symptoms could lead to a new agro-technology to avoid chilling injuries and maintain fruit quality during cold storage at the lowest possible temperature.

  6. Storing a single photon as a spin wave entangled with a flying photon in the telecommunication bandwidth

    NASA Astrophysics Data System (ADS)

    Zhang, Wei; Ding, Dong-Sheng; Shi, Shuai; Li, Yan; Zhou, Zhi-Yuan; Shi, Bao-Sen; Guo, Guang-Can

    2016-02-01

    Quantum memory is an essential building block for quantum communication and scalable linear quantum computation. Storing two-color entangled photons with one photon being at the telecommunication (telecom) wavelength while the other photon is compatible with quantum memory has great advantages toward the realization of the fiber-based long-distance quantum communication with the aid of quantum repeaters. Here, we report an experimental realization of storing a photon entangled with a telecom photon in polarization as an atomic spin wave in a cold atomic ensemble, thus establishing the entanglement between the telecom-band photon and the atomic-ensemble memory in a polarization degree of freedom. The reconstructed density matrix and the violation of the Clauser-Horne-Shimony-Holt inequality clearly show the preservation of quantum entanglement during storage. Our result is very promising for establishing a long-distance quantum network based on cold atomic ensembles.

  7. Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage

    PubMed Central

    Brizzolara, Stefano; Hertog, Maarten; Tosetti, Roberta; Nicolai, Bart; Tonutti, Pietro

    2018-01-01

    Refrigerated storage is widely applied in order to maintain peach quality but it can also induce chilling injuries (CIs) such as flesh browning and bleeding, and mealiness. Peach fruit from three cultivars (‘Red Haven’, RH, ‘Regina di Londa’, RL, and ‘Flaminia’, FL) were stored for 4 weeks under low temperatures (0.5 and 5.5°C). GC-MS was employed to study changes in both metabolome and volatilome induced by cold storage in the mesocarp. CIs were assessed both at the end of each week of storage and after subsequent shelf-life (SL) at 20°C. Flesh browning and mealiness appeared to be more related to 5.5°C storage, while flesh bleeding revealed high incidence following 0.5°C storage. Compared to RL and FL, RH showed a marked lower incidence of CIs. Multivariate statistical analyses indicate that RH peaches indeed differ from RL and FL in particular when considering data from samples collected at the end of the cold storage. Common and divergent responses have been identified in terms of metabolic responses to the applied low temperatures. In all three cultivars raffinose, glucose-6P, fucose, xylose, sorbitol, GABA, epicatechin, catechin, and putrescine markedly increased during cold storage, while citramalic, glucuronic, mucic and shikimic acids decreased. Among volatile organic compounds (VOCs), aldehydes and alcohols generally accumulated more under low temperature conditions while esters and lactones evolved during subsequent SL. The main cultivar differences developed after cold storage during SL although some common responses (e.g., an increased production of ethyl acetate) were observed. The lower levels of flesh browning and bleeding displayed by RH peaches were related to compounds with antioxidant activity, or acting as osmotic protectants and membrane stabilizer. Indeed, RH showed higher levels of amino acids and urea, together with a marked increase in putrescine, sorbitol, maltitol, myoinositol and sucrose detected during storage and SL. PMID:29892309

  8. Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage.

    PubMed

    Brizzolara, Stefano; Hertog, Maarten; Tosetti, Roberta; Nicolai, Bart; Tonutti, Pietro

    2018-01-01

    Refrigerated storage is widely applied in order to maintain peach quality but it can also induce chilling injuries (CIs) such as flesh browning and bleeding, and mealiness. Peach fruit from three cultivars ('Red Haven', RH, 'Regina di Londa', RL, and 'Flaminia', FL) were stored for 4 weeks under low temperatures (0.5 and 5.5°C). GC-MS was employed to study changes in both metabolome and volatilome induced by cold storage in the mesocarp. CIs were assessed both at the end of each week of storage and after subsequent shelf-life (SL) at 20°C. Flesh browning and mealiness appeared to be more related to 5.5°C storage, while flesh bleeding revealed high incidence following 0.5°C storage. Compared to RL and FL, RH showed a marked lower incidence of CIs. Multivariate statistical analyses indicate that RH peaches indeed differ from RL and FL in particular when considering data from samples collected at the end of the cold storage. Common and divergent responses have been identified in terms of metabolic responses to the applied low temperatures. In all three cultivars raffinose, glucose-6P, fucose, xylose, sorbitol, GABA, epicatechin, catechin, and putrescine markedly increased during cold storage, while citramalic, glucuronic, mucic and shikimic acids decreased. Among volatile organic compounds (VOCs), aldehydes and alcohols generally accumulated more under low temperature conditions while esters and lactones evolved during subsequent SL. The main cultivar differences developed after cold storage during SL although some common responses (e.g., an increased production of ethyl acetate) were observed. The lower levels of flesh browning and bleeding displayed by RH peaches were related to compounds with antioxidant activity, or acting as osmotic protectants and membrane stabilizer. Indeed, RH showed higher levels of amino acids and urea, together with a marked increase in putrescine, sorbitol, maltitol, myoinositol and sucrose detected during storage and SL.

  9. Overexpression of the long noncoding RNA TUG1 protects against cold-induced injury of mouse livers by inhibiting apoptosis and inflammation.

    PubMed

    Su, Song; Liu, Jiang; He, Kai; Zhang, Mengyu; Feng, Chunhong; Peng, Fangyi; Li, Bo; Xia, Xianming

    2016-04-01

    Hepatic injury provoked by cold storage is a major problem affecting liver transplantation, as exposure to cold induces apoptosis in hepatic tissues. Long noncoding RNAs (lncRNAs) are increasingly understood to regulate apoptosis, but the contribution of lncRNAs to cold-induced liver injury remains unknown. Using RNA-seq, we determined the differential lncRNA expression profile in mouse livers after cold storage and found that expression of the lncRNA TUG1 was significantly down-regulated. Overexpression of TUG1 attenuated cold-induced apoptosis in mouse hepatocytes and liver sinusoidal endothelial cells LSECs, in part by blocking mitochondrial apoptosis and endoplasmic reticulum (ER) stress pathways. Moreover, TUG1 attenuated apoptosis, inflammation, and oxidative stress in vivo in livers subjected to cold storage. Overexpression of TUG1 also improved hepatocyte function and prolonged hepatic graft survival rates in mice. These results suggest that the lncRNA TUG1 exerts a protective effect against cold-induced liver damage by inhibiting apoptosis in mice, and suggests a potential role for TUG1 as a target for the prevention of cold-induced liver damage in liver transplantation. RNA-seq data are available from GEO using accession number GSE76609. © 2016 Federation of European Biochemical Societies.

  10. Practical and efficient magnetic heat pump

    NASA Technical Reports Server (NTRS)

    Brown, G. V.

    1978-01-01

    Method for pumping heat magnetically at room temperature is more economical than existing refrigeration systems. Method uses natural magneto-thermal effect of gadolinium metal to establish temperature gradient across length of tube. Regenerative cyclic process in which gadolinium sample is magnetized and gives off heat at one end of tube, and then is demagnetized at other end to absorb heat has established temperature gradients of 144 degrees F in experiments near room temperature. Other materials with large magnetothermal effects can be used below room temperature. Possible commercial applications include freeze-drying and food processing, cold storage, and heating and cooling of buildings, plants, and ships.

  11. A Bulk Segregant Gene Expression Analysis of a Peach Population Reveals Components of the Underlying Mechanism of the Fruit Cold Response

    PubMed Central

    Pons, Clara; Martí, Cristina; Forment, Javier; Crisosto, Carlos H.; Dandekar, Abhaya M.; Granell, Antonio

    2014-01-01

    Peach fruits subjected for long periods of cold storage are primed to develop chilling injury once fruits are shelf ripened at room temperature. Very little is known about the molecular changes occurring in fruits during cold exposure. To get some insight into this process a transcript profiling analyses was performed on fruits from a PopDG population segregating for chilling injury CI responses. A bulked segregant gene expression analysis based on groups of fruits showing extreme CI responses indicated that the transcriptome of peach fruits was modified already during cold storage consistently with eventual CI development. Most peach cold-responsive genes have orthologs in Arabidopsis that participate in cold acclimation and other stresses responses, while some of them showed expression patterns that differs in fruits according to their susceptibility to develop mealiness. Members of ICE1, CBF1/3 and HOS9 regulons seem to have a prominent role in differential cold responses between low and high sensitive fruits. In high sensitive fruits, an alternative cold response program is detected. This program is probably associated with dehydration/osmotic stress and regulated by ABA, auxins and ethylene. In addition, the observation that tolerant siblings showed a series of genes encoding for stress protective activities with higher expression both at harvest and during cold treatment, suggests that preprogrammed mechanisms could shape fruit ability to tolerate postharvest cold-induced stress. A number of genes differentially expressed were validated and extended to individual genotypes by medium-throughput RT-qPCR. Analyses presented here provide a global view of the responses of peach fruits to cold storage and highlights new peach genes that probably play important roles in the tolerance/sensitivity to cold storage. Our results provide a roadmap for further experiments and would help to develop new postharvest protocols and gene directed breeding strategies to better cope with chilling injury. PMID:24598973

  12. A bulk segregant gene expression analysis of a peach population reveals components of the underlying mechanism of the fruit cold response.

    PubMed

    Pons, Clara; Martí, Cristina; Forment, Javier; Crisosto, Carlos H; Dandekar, Abhaya M; Granell, Antonio

    2014-01-01

    Peach fruits subjected for long periods of cold storage are primed to develop chilling injury once fruits are shelf ripened at room temperature. Very little is known about the molecular changes occurring in fruits during cold exposure. To get some insight into this process a transcript profiling analyses was performed on fruits from a PopDG population segregating for chilling injury CI responses. A bulked segregant gene expression analysis based on groups of fruits showing extreme CI responses indicated that the transcriptome of peach fruits was modified already during cold storage consistently with eventual CI development. Most peach cold-responsive genes have orthologs in Arabidopsis that participate in cold acclimation and other stresses responses, while some of them showed expression patterns that differs in fruits according to their susceptibility to develop mealiness. Members of ICE1, CBF1/3 and HOS9 regulons seem to have a prominent role in differential cold responses between low and high sensitive fruits. In high sensitive fruits, an alternative cold response program is detected. This program is probably associated with dehydration/osmotic stress and regulated by ABA, auxins and ethylene. In addition, the observation that tolerant siblings showed a series of genes encoding for stress protective activities with higher expression both at harvest and during cold treatment, suggests that preprogrammed mechanisms could shape fruit ability to tolerate postharvest cold-induced stress. A number of genes differentially expressed were validated and extended to individual genotypes by medium-throughput RT-qPCR. Analyses presented here provide a global view of the responses of peach fruits to cold storage and highlights new peach genes that probably play important roles in the tolerance/sensitivity to cold storage. Our results provide a roadmap for further experiments and would help to develop new postharvest protocols and gene directed breeding strategies to better cope with chilling injury.

  13. Improved biochemical preservation of heart slices during cold storage.

    PubMed

    Bull, D A; Reid, B B; Connors, R C; Albanil, A; Stringham, J C; Karwande, S V

    2000-01-01

    Development of myocardial preservation solutions requires the use of whole organ models which are animal and labor intensive. These models rely on physiologic rather than biochemical endpoints, making accurate comparison of the relative efficacy of individual solution components difficult. We hypothesized that myocardial slices could be used to assess preservation of biochemical function during cold storage. Whole rat hearts were precision cut into slices with a thickness of 200 microm and preserved at 4 degrees C in one of the following solutions: Columbia University (CU), University of Wisconsin (UW), D5 0.2% normal saline with 20 meq/l KCL (QNS), normal saline (NS), or a novel cardiac preservation solution (NPS) developed using this model. Myocardial biochemical function was assessed by ATP content (etamoles ATP/mg wet weight) and capacity for protein synthesis (counts per minute (cpm)/mg protein) immediately following slicing (0 hours), and at 6, 12, 18, and 24 hours of cold storage. Six slices were assayed at each time point for each solution. The data were analyzed using analysis of variance and are presented as the mean +/- standard deviation. ATP content was higher in the heart slices stored in the NPS compared to all other solutions at 6, 12, 18 and 24 hours of cold storage (p < 0.05). Capacity for protein synthesis was higher in the heart slices stored in the NPS compared to all other solutions at 6, 12, and 18 hours of cold storage (p < 0.05). CONCLUSIONS This myocardial slice model allows the rapid and efficient screening of cardiac preservation solutions and their components using quantifiable biochemical endpoints. Using this model, we have developed a novel preservation solution which improves the biochemical function of myocardial slices during cold storage.

  14. Analysis of ethylene biosynthesis and perception during postharvest cold storage of Marsh and Star Ruby grapefruits.

    PubMed

    Lado, Joanna; Rodrigo, María Jesús; Zacarías, Lorenzo

    2015-10-01

    Grapefruits are among the citrus species more sensitive to cold and develop chilling injury symptoms during prolonged postharvest storage at temperatures lower than 8 ℃-10 ℃. The plant hormone ethylene has been described either to protect or potentiate chilling injury development in citrus whereas little is known about transcriptional regulation of ethylene biosynthesis, perception and response during cold storage and how the hormone is regulating its own perception and signaling cascade. Then, the objective of the present study was to explore the transcriptional changes in the expression of ethylene biosynthesis, receptors and response genes during cold storage of the white Marsh and the red Star Ruby grapefruits. The effect of the ethylene action inhibitor, 1-MCP, was evaluated to investigate the involvement of ethylene in the regulation of the genes of its own biosynthesis and perception pathway. Ethylene production was very low at the harvest time in fruits of both varieties and experienced only minor changes during storage. By contrast, inhibition of ethylene perception by 1-MCP markedly induced ethylene production, and this increase was highly stimulated during shelf-life at 20 ℃, as well as transcription of ACS and ACO. These results support the auto-inhibitory regulation of ethylene in grapefruits, which acts mainly at the transcriptional level of ACS and ACO genes. Moreover, ethylene receptor1 and ethylene receptor3 were induced by cold while no clear role of ethylene was observed in the induction of ethylene receptors. However, ethylene appears to be implicated in the transcriptional regulation of ERFs both under cold storage and shelf-life. © The Author(s) 2014.

  15. iss050e057428

    NASA Image and Video Library

    2017-03-15

    iss050e057428 (03/15/2017) --- NASA astronaut Shane Kimbrough removes a storage locker in the Minus Eighty-degree Laboratory Freezer for ISS (MELFI) to store samples from an experiment. MELFI is a cold storage unit that maintains experiment samples at ultra-cold temperatures throughout a mission.

  16. Temporal Cold Storage of Eggs of the Poplar Tent Maker, Clostera inclusa, Prior to Use in Rearing the Egg Parasite, Ooencyrfus ennomophagus

    Treesearch

    A.T. Drooz; J.D. Solomon

    1984-01-01

    After being retained in cold storage at -10°C over a 24-month period, some Clostera inclusa (Hubner) eggs were still able to be successfully parasitized by Ooencyrtus ennomophagus Yoshimoto, an egg parasite. An equation was developed for predicting parasite yield over time from cold-stored eggs. Predicted parasitism was 25 percent or better for up...

  17. Proteomic Analysis of Differentially Accumulated Proteins in Cucumber (Cucumis sativus) Fruit Peel in Response to Pre-storage Cold Acclimation

    PubMed Central

    Wang, Bin; Shen, Fei; Zhu, Shijiang

    2018-01-01

    Harvested fruits are still living organs and respond to environmental stimuli. Low temperature storage is effective in extending life of harvested fruit, but it may also cause chilling injury. Cold acclimation has been shown to induce chilling tolerance in plants, but what proteomic changes caused by cold acclimation are related to defense against chilling stress remains largely unclear. Here, 3 d of pre-storage cold acclimation (PsCA) at 10°C reduced chilling injury and secondary disease severity in cucumber stored at 5°C by 51 and 94%, respectively, compared with the control which was directly stored at 5°C. Proteomic analysis of cucumber peel identified 21 significant differentially-accumulated proteins (SDAPs) right after PsCA treatment and 23 after the following cold storage (PsCA+CS). These proteins are mainly related to stress response and defense (SRD), energy metabolism, protein metabolism, signal transduction, primary metabolism, and transcription. The SRD proteins, which made up 37% of the 21 and 47% of the 23, respectively, represented the largest class of SDAPs, and all but one protein were up-regulated, suggesting accumulation of proteins involved in defense response is central feature of proteomic profile changes brought about by PsCA. In fruit just after PsCA treatment, the identified SDAPs are related to responses to various stresses, including chilling, salt stress, dehydration, fungi, bacteria, insects, and DNA damage. However, after prolonged cold storage, the targeted proteins in acclimated fruit were narrowed down in scope to those involved in defense against chilling and pathogens. The change patterns at the transcription level of the majority of the up-regulated differentially-accumulated proteins were highly consistent with those at protein level. Taken all, the results suggest that the short-time cold acclimation initiated comprehensive defense responses in cucumber fruit at first, while the long term storage thereafter altered the responses more specifically to chilling. These findings add to the understanding of plants' molecular responses to cold acclimation. PMID:29403505

  18. Proteomic Analysis of Differentially Accumulated Proteins in Cucumber (Cucumis sativus) Fruit Peel in Response to Pre-storage Cold Acclimation.

    PubMed

    Wang, Bin; Shen, Fei; Zhu, Shijiang

    2017-01-01

    Harvested fruits are still living organs and respond to environmental stimuli. Low temperature storage is effective in extending life of harvested fruit, but it may also cause chilling injury. Cold acclimation has been shown to induce chilling tolerance in plants, but what proteomic changes caused by cold acclimation are related to defense against chilling stress remains largely unclear. Here, 3 d of pre-storage cold acclimation (PsCA) at 10°C reduced chilling injury and secondary disease severity in cucumber stored at 5°C by 51 and 94%, respectively, compared with the control which was directly stored at 5°C. Proteomic analysis of cucumber peel identified 21 significant differentially-accumulated proteins (SDAPs) right after PsCA treatment and 23 after the following cold storage (PsCA+CS). These proteins are mainly related to stress response and defense (SRD), energy metabolism, protein metabolism, signal transduction, primary metabolism, and transcription. The SRD proteins, which made up 37% of the 21 and 47% of the 23, respectively, represented the largest class of SDAPs, and all but one protein were up-regulated, suggesting accumulation of proteins involved in defense response is central feature of proteomic profile changes brought about by PsCA. In fruit just after PsCA treatment, the identified SDAPs are related to responses to various stresses, including chilling, salt stress, dehydration, fungi, bacteria, insects, and DNA damage. However, after prolonged cold storage, the targeted proteins in acclimated fruit were narrowed down in scope to those involved in defense against chilling and pathogens. The change patterns at the transcription level of the majority of the up-regulated differentially-accumulated proteins were highly consistent with those at protein level. Taken all, the results suggest that the short-time cold acclimation initiated comprehensive defense responses in cucumber fruit at first, while the long term storage thereafter altered the responses more specifically to chilling. These findings add to the understanding of plants' molecular responses to cold acclimation.

  19. Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats.

    PubMed

    Perez, Rogério Renato; Goldenberg, Alberto; Netto, Alcides Augusto Salzedas; Gonzalez, Adriano Miziara

    2014-03-01

    To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia. Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage. Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups. The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia.

  20. Cryogenic on-orbit liquid depot storage acquisition and transfer (COLD-SAT) experiment subsystem instrumentation and wire harness design report

    NASA Technical Reports Server (NTRS)

    Edwards, Lawrence G.

    1994-01-01

    Subcritical cryogens such as liquid hydrogen (LH2) and liquid oxygen (LO2) are required for space based transportation propellant, reactant, and life support systems. Future long-duration space missions will require on-orbit systems capable of long-term cryogen storage and efficient fluid transfer capabilities. COLD-SAT, which stands for cryogenic orbiting liquid depot-storage acquisition and transfer, is a free-flying liquid hydrogen management flight experiment. Experiments to determine optimum methods of fluid storage and transfer will be performed on the COLD-SAT mission. The success of the mission is directly related to the type and accuracy of measurements made. The instrumentation and measurement techniques used are therefore critical to the success of the mission. This paper presents the results of the COLD-SAT experiment subsystem instrumentation and wire harness design effort. Candidate transducers capable of fulfilling the COLD-SAT experiment measurement requirements are identified. Signal conditioning techniques, data acquisition requirements, and measurement uncertainty analysis are presented. Electrical harnessing materials and wiring techniques for the instrumentation designed to minimize heat conduction to the cryogenic tanks and provide optimum measurement accuracy are listed.

  1. 71. INTERIOR, BUILDING 272 (PLUTONIUM STORAGE BUILDING) LOOKING NORTHEAST INTO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    71. INTERIOR, BUILDING 272 (PLUTONIUM STORAGE BUILDING) LOOKING NORTHEAST INTO PLUTONIUM STORAGE ROOM SHOWING CUBICLES FOR STORAGE. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  2. 70. INTERIOR, BUILDING 272 (PLUTONIUM STORAGE BUILDING) LOOKING WEST INTO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    70. INTERIOR, BUILDING 272 (PLUTONIUM STORAGE BUILDING) LOOKING WEST INTO STORAGE AREA SHOWING THE FOUR STORAGE ROOM ENTRANCES. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  3. INTERIOR OF WESTERN SECTION, SHOWING WALL OF COLD STORAGE ROOM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR OF WESTERN SECTION, SHOWING WALL OF COLD STORAGE ROOM (IN BAYS 32 TO 34) AND ROLLING DOORS AT WEST END, VIEW FACING SOUTH-SOUTHWEST. - Naval Air Station Barbers Point, Aircraft Storehouse, Between Midway & Card Streets at Enterprise Avenue intersection, Ewa, Honolulu County, HI

  4. The effect of melanin-free extract from Sepia esculenta ink on lipid peroxidation, protein oxidation and water-holding capacity of tilapia fillet during cold storage.

    PubMed

    Duan, Zhen-Hua; Liu, Hua-Zhong; Luo, Ping; Gu, Yi-Peng; Li, Yan-Qun

    2018-03-14

    Preservative effect of melanin-free extract of Sepia esculenta ink (MFESI) on Sparus latus fillet has been verified in our previous work. This study aims to further approach the mechanism of MFESI for extending the shelf-life of fish fillet during cold storage. Tilapia fillets were treated with different dosage of MFESI (0, 15, 25 and 35 mg/ml) and packed with preservative film for succedent cold-storage at 4 °C for scheduled time. Contents of total volatile basic nitrogen and sulfydryl and carbanyl groups were measured for evaluating protein oxidation. Malondialdehyde contents were measured for estimating lipid peroxidation and loss of water was used to determine water-holding capacity of fillet. The data indicated that MFESI not only possessed certain degree of antioxidant capacity in vitro, also lengthened shelf-life of tilapia fillet in cold-storage condition. Apart from 15 mg/ml, both 25 and 35 mg/ml of MFESI obviously prevented lipid and protein from oxidation and reduced loss of water from tilapia fillets, and the latter was more effective than the former. MFESI can repress lipid peroxidation and protein oxidation and reduce water loss, maintain the tilapia fillets quality and, thus, it could be an effective and natural preservative for extending the shelf-life of tilapia fillets during cold storage.

  5. Photonic quantum state transfer between a cold atomic gas and a crystal.

    PubMed

    Maring, Nicolas; Farrera, Pau; Kutluer, Kutlu; Mazzera, Margherita; Heinze, Georg; de Riedmatten, Hugues

    2017-11-22

    Interfacing fundamentally different quantum systems is key to building future hybrid quantum networks. Such heterogeneous networks offer capabilities superior to those of their homogeneous counterparts, as they merge the individual advantages of disparate quantum nodes in a single network architecture. However, few investigations of optical hybrid interconnections have been carried out, owing to fundamental and technological challenges such as wavelength and bandwidth matching of the interfacing photons. Here we report optical quantum interconnection of two disparate matter quantum systems with photon storage capabilities. We show that a quantum state can be transferred faithfully between a cold atomic ensemble and a rare-earth-doped crystal by means of a single photon at 1,552  nanometre telecommunication wavelength, using cascaded quantum frequency conversion. We demonstrate that quantum correlations between a photon and a single collective spin excitation in the cold atomic ensemble can be transferred to the solid-state system. We also show that single-photon time-bin qubits generated in the cold atomic ensemble can be converted, stored and retrieved from the crystal with a conditional qubit fidelity of more than 85 per cent. Our results open up the prospect of optically connecting quantum nodes with different capabilities and represent an important step towards the realization of large-scale hybrid quantum networks.

  6. Delayed response to cold stress is characterized by successive metabolic shifts culminating in apple fruit peel necrosis.

    PubMed

    Gapper, Nigel E; Hertog, Maarten L A T M; Lee, Jinwook; Buchanan, David A; Leisso, Rachel S; Fei, Zhangjun; Qu, Guiqin; Giovannoni, James J; Johnston, Jason W; Schaffer, Robert J; Nicolaï, Bart M; Mattheis, James P; Watkins, Christopher B; Rudell, David R

    2017-04-21

    Superficial scald is a physiological disorder of apple fruit characterized by sunken, necrotic lesions appearing after prolonged cold storage, although initial injury occurs much earlier in the storage period. To determine the degree to which the transition to cell death is an active process and specific metabolism involved, untargeted metabolic and transcriptomic profiling was used to follow metabolism of peel tissue over 180 d of cold storage. The metabolome and transcriptome of peel destined to develop scald began to diverge from peel where scald was controlled using antioxidant (diphenylamine; DPA) or rendered insensitive to ethylene using 1-methylcyclopropene (1-MCP) beginning between 30 and 60 days of storage. Overall metabolic and transcriptomic shifts, representing multiple pathways and processes, occurred alongside α-farnesene oxidation and, later, methanol production alongside symptom development. Results indicate this form of peel necrosis is a product of an active metabolic transition involving multiple pathways triggered by chilling temperatures at cold storage inception rather than physical injury. Among multiple other pathways, enhanced methanol and methyl ester levels alongside upregulated pectin methylesterases are unique to peel that is developing scald symptoms similar to injury resulting from mechanical stress and herbivory in other plants.

  7. Only adding stationary storage to vaccine supply chains may create and worsen transport bottlenecks.

    PubMed

    Haidari, Leila A; Connor, Diana L; Wateska, Angela R; Brown, Shawn T; Mueller, Leslie E; Norman, Bryan A; Schmitz, Michelle M; Paul, Proma; Rajgopal, Jayant; Welling, Joel S; Leonard, Jim; Claypool, Erin G; Weng, Yu-Ting; Chen, Sheng-I; Lee, Bruce Y

    2013-01-01

    Although vaccine supply chains in many countries require additional stationary storage and transport capacity to meet current and future needs, international donors tend to donate stationary storage devices far more often than transport equipment. To investigate the impact of only adding stationary storage equipment on the capacity requirements of transport devices and vehicles, we used HERMES (Highly Extensible Resource for Modeling Supply Chains) to construct a discrete event simulation model of the Niger vaccine supply chain. We measured the transport capacity requirement for each mode of transport used in the Niger vaccine cold chain, both before and after adding cold rooms and refrigerators to relieve all stationary storage constraints in the system. With the addition of necessary stationary storage, the average transport capacity requirement increased from 88% to 144% for cold trucks, from 101% to 197% for pickup trucks, and from 366% to 420% for vaccine carriers. Therefore, adding stationary storage alone may worsen or create new transport bottlenecks as more vaccines flow through the system, preventing many vaccines from reaching their target populations. Dynamic modeling can reveal such relationships between stationary storage capacity and transport constraints.

  8. Only Adding Stationary Storage to Vaccine Supply Chains May Create and Worsen Transport Bottlenecks

    PubMed Central

    Haidari, Leila A.; Connor, Diana L.; Wateska, Angela R.; Brown, Shawn T.; Mueller, Leslie E.; Norman, Bryan A.; Schmitz, Michelle M.; Paul, Proma; Rajgopal, Jayant; Welling, Joel S.; Leonard, Jim; Claypool, Erin G.; Weng, Yu-Ting; Chen, Sheng-I; Lee, Bruce Y.

    2015-01-01

    Although vaccine supply chains in many countries require additional stationary storage and transport capacity to meet current and future needs, international donors tend to donate stationary storage devices far more often than transport equipment. To investigate the impact of only adding stationary storage equipment on the capacity requirements of transport devices and vehicles, we used HERMES (Highly Extensible Resource for Modeling Supply Chains) to construct a discrete event simulation model of the Niger vaccine supply chain. We measured the transport capacity requirement for each mode of transport used in the Niger vaccine cold chain, both before and after adding cold rooms and refrigerators to relieve all stationary storage constraints in the system. With the addition of necessary stationary storage, the average transport capacity requirement increased from 88% to 144% for cold trucks, from 101% to 197% for pickup trucks, and from 366% to 420% for vaccine carriers. Therefore, adding stationary storage alone may worsen or create new transport bottlenecks as more vaccines flow through the system, preventing many vaccines from reaching their target populations. Dynamic modeling can reveal such relationships between stationary storage capacity and transport constraints. PMID:23903398

  9. Cryogenic On-Orbit Liquid Depot Storage, Acquisition, and Transfer satellite (COLD-SAT) feasibility study

    NASA Technical Reports Server (NTRS)

    Bailey, William J.; Weiner, Stephen P.; Beekman, Douglas H.; Dennis, Mark F.; Martin, Timothy A.

    1990-01-01

    The Cryogenic On-Orbit Liquid Depot Storage, Acquisition, and Transfer Satellite (COLD-SAT) is an experimental spacecraft launched from an expendable launch vehicle which is designed to investigate the systems and technologies required for efficient, effective, and reliable management of cryogenic fluid in the reduced gravity space environment. The COLD-SAT program will provide the necessary data base and provide low-g proving of fluid and thermal models of cryogenic storage, transfer, and resupply concepts and processes. A conceptual approach was developed and an overview of the results of the 24 month COLD-SAT Phase A feasibility is described which includes: (1) a definition of the technology needs and the accompanying experimental 3 month baseline mission; (2) a description of the experiment subsystem, major features and rationale for satisfaction of primary and secondary experiment requirements using liquid hydrogen as the test fluid; and (3) a presentation of the conceptual design of the COLD-SAT spacecraft subsystems which support the on-orbit experiment with emphasis on areas of greatest challenge.

  10. VIEW OF CEMETERY SECTIONS B AND A, WITH LODGE BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF CEMETERY SECTIONS B AND A, WITH LODGE BUILDING AT RIGHT BACKGROUND AND MAINTENANCE BUILDING FAR LEFT BACKGROUND. VIEW TO NORTHEAST. - Cold Harbor National Cemetery, 6038 Cold Harbor Road, Mechanicsville, Hanover County, VA

  11. Up-regulation of genes in diphenylamine- and 1-methylcyclopropene-treated apples during cold storage

    USDA-ARS?s Scientific Manuscript database

    Cold storage reduces the rate of quality loss and extends availability of fresh apples in the marketplace, but several cultivars develop various postharvest browning disorders of the peel or flesh tissue such as superficial scald and external carbon dioxide injury. Postharvest 1-methylcyclopropene...

  12. US vaccine refrigeration guidelines: loose links in the cold chain.

    PubMed

    McColloster, Patrick J

    2011-05-01

    This commentary compares Centers for Disease Control (CDC) guidelines for vaccine storage with international cold chain standards. Problems related to the use of domestic refrigerators in clinical settings are discussed. Optimal vaccine refrigerator design characteristics are summarized. The adoption of World Health Organization storage recommendations is advised.

  13. Transcriptome Dynamics in Mango Fruit Peel Reveals Mechanisms of Chilling Stress

    PubMed Central

    Sivankalyani, Velu; Sela, Noa; Feygenberg, Oleg; Zemach, Hanita; Maurer, Dalia; Alkan, Noam

    2016-01-01

    Cold storage is considered the most effective method for prolonging fresh produce storage. However, subtropical fruit is sensitive to cold. Symptoms of chilling injury (CI) in mango include red and black spots that start from discolored lenticels and develop into pitting. The response of ‘Keitt’ mango fruit to chilling stress was monitored by transcriptomic, physiological, and microscopic analyses. Transcriptomic changes in the mango fruit peel were evaluated during optimal (12°C) and suboptimal (5°C) cold storage. Two days of chilling stress upregulated genes involved in the plant stress response, including those encoding transmembrane receptors, calcium-mediated signal transduction, NADPH oxidase, MAP kinases, and WRKYs, which can lead to cell death. Indeed, cell death was observed around the discolored lenticels after 19 days of cold storage at 5°C. Localized cell death and cuticular opening in the lumen of discolored lenticels were correlated with increased general decay during shelf-life storage, possibly due to fungal penetration. We also observed increased phenolics accumulation around the discolored lenticels, which was correlated with the biosynthesis of phenylpropanoids that were probably transported from the resin ducts. Increased lipid peroxidation was observed during CI by both the biochemical malondialdehyde method and a new non-destructive luminescent technology, correlated to upregulation of the α-linolenic acid oxidation pathway. Genes involved in sugar metabolism were also induced, possibly to maintain osmotic balance. This analysis provides an in-depth characterization of mango fruit response to chilling stress and could lead to the development of new tools, treatments and strategies to prolong cold storage of subtropical fruit. PMID:27812364

  14. Cost effective propulsion systems for small satellites using butane propellant

    NASA Astrophysics Data System (ADS)

    Gibbon, D.; Underwood, C.; Sweeting, M.; Amri, R.

    2002-07-01

    This paper will describe the work performed at the Surrey Space Centre to produce cost effective propulsion systems for small spacecraft with relatively low deltaV (ΔV) requirements. Traditionally, cold gas nitrogen systems have been used for this type of application, however they have high storage volume requirements. This can be a problem on small spacecraft, which are typically volume limited. An alternative solution is to use liquefied gases, which store as liquids, hence have reasonable density levels, and can be used in a cold gas thruster. At the Surrey Space Centre, butane has been selected as the propellant of choice. Although it has slightly lower specific impulse performance than nitrogen, it has a significantly higher storage density and it stores at a very low pressure, hence no regulation system is required. On 28 th June 2000 Surrey Satellite Technology Ltd (SSTL) launched it first nanosatellite SNAP-1. This 6.5kg spacecraft was equipped with a small cold gas propulsion system utilising 32.6 grams of butane propellant. During the propulsion system operation phase the spacecraft's semi major axis was raised by nearly 4 kilometers using the propulsion system. The design of the propulsion system will be described and the low cost features highlighted. Telemetry data will be used to describe the propulsion operations and an overall mission specific impulse will be derived. SSTL are currently under contract to build three Earth observation spacecraft for a Disaster Monitoring Constellation (DMC). Each spacecraft will weigh approx 100 kg and have a ΔV requirement of 10 m/sec. A butane system has been designed and manufactured to meet the requirements of these spacecraft. The system is based very much on the flight heritage of the SNAP-1 system, with the addition of greater propellant storage capacity. The lessons learnt from the SNAP-1 operation will be reviewed and the resulting design improvements on the DMC propulsion systems will be detailed.

  15. Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium - identification of the responsible medium components.

    PubMed

    Pless-Petig, Gesine; Metzenmacher, Martin; Türk, Tobias R; Rauen, Ursula

    2012-10-10

    In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells, surprisingly, strongly enhanced cold-induced cell injury in these cells in comparison to cold storage in Krebs-Henseleit buffer or other cell culture media (DMEM, L-15 and M199). Manufacturer, batch, medium supplements and the most likely components with concentrations outside the range of the other media/solutions (vitamin B12, inositol, biotin, p-aminobenzoic acid) did not cause this aggravation of cold-induced injury in RPMI 1640. However, a modified Krebs-Henseleit buffer with a low calcium concentration (0.42 mM), a high concentration of inorganic phosphate (5.6 mM), and glucose (11.1 mM; i.e. concentrations as in RPMI 1640) evoked a cell injury and loss of metabolic function corresponding to that observed in RPMI 1640. Deferoxamine improved cell survival and preserved metabolic function in modified Krebs-Henseleit buffer as well as in RPMI 1640. Similar Ca2+ and phosphate concentrations did not increase cold-induced cell injury in the kidney cell line LLC-PK1, porcine aortic endothelial cells or rat hepatocytes. However, more extreme conditions (Ca2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. These data suggest that the combination of low calcium and high phosphate concentrations in the presence of glucose enhances cold-induced, iron-dependent injury drastically in Vero-B4 cells, and that a tendency for this pathomechanism also exists in other cell types.

  16. WEAPONS STORAGE AREA, LOOKING TOWARD ELECTRIC POWER STATION BUILDING (BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEAPONS STORAGE AREA, LOOKING TOWARD ELECTRIC POWER STATION BUILDING (BUILDING 3583), STORAGE BUILDING (BUILDING 3584)NIGHT AND SECURITY POLICE ENTRY CONTROL (BUILDING 3582)LEFT. VIEW TO NORTHEAST - Plattsburgh Air Force Base, U.S. Route 9, Plattsburgh, Clinton County, NY

  17. Model of refrigerated display-space allocation for multi agro-perishable products considering markdown policy

    NASA Astrophysics Data System (ADS)

    Satiti, D.; Rusdiansyah, A.

    2018-04-01

    Problems that need more attention in the agri-food supply chain are loss and waste as consequences from improper quality control and excessive inventories. The use of cold storage is still being one of favourite technologies in controlling product quality by majority of retailers. We considerate the temperature of cold storage in determining the inventory and pricing strategies based on identified product quality. This study aims to minimize the agri-food waste, utility of cold storage facilities and maximize retailer’s profit through determining the refrigerated display-space allocation and markdown policy based on identified food shelf life. The proposed model evaluated with several different scenarios to find out the right strategy.

  18. 69. INTERIOR, BUILDING 272 (PLUTONIUM STORAGE BUILDING) LOOKING SOUTHWEST THROUGH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    69. INTERIOR, BUILDING 272 (PLUTONIUM STORAGE BUILDING) LOOKING SOUTHWEST THROUGH DOOR-WAY INTO PLUTONIUM STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  19. 30 CFR 56.4531 - Flammable or combustible liquid storage buildings or rooms.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Flammable or combustible liquid storage... combustible liquid storage buildings or rooms. (a) Storage buildings or storage rooms in which flammable or... no person's work station is in the building. (c) Flammable or combustible liquids in use for day-to...

  20. 30 CFR 56.4531 - Flammable or combustible liquid storage buildings or rooms.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Flammable or combustible liquid storage... combustible liquid storage buildings or rooms. (a) Storage buildings or storage rooms in which flammable or... no person's work station is in the building. (c) Flammable or combustible liquids in use for day-to...

  1. 30 CFR 56.4531 - Flammable or combustible liquid storage buildings or rooms.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Flammable or combustible liquid storage... combustible liquid storage buildings or rooms. (a) Storage buildings or storage rooms in which flammable or... no person's work station is in the building. (c) Flammable or combustible liquids in use for day-to...

  2. 30 CFR 56.4531 - Flammable or combustible liquid storage buildings or rooms.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Flammable or combustible liquid storage... combustible liquid storage buildings or rooms. (a) Storage buildings or storage rooms in which flammable or... no person's work station is in the building. (c) Flammable or combustible liquids in use for day-to...

  3. 3. Photocopy of photograph (Original print, Phillip McCracken, courtesy of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopy of photograph (Original print, Phillip McCracken, courtesy of Bill Mitchell.) Photographer unknown, 1924. Cold Storage Warehouse on the left, north and west facades. On the right, north facade of the Hay and Grain Warehouse. - Curtis Wharf, Cold Storage Warehouse, O & Second Streets, Anacortes, Skagit County, WA

  4. Delayed response to cold stress is characterized by successive metabolic shifts culminating in apple fruit peel necrosis

    USDA-ARS?s Scientific Manuscript database

    Superficial scald is a physiological disorder of apple fruit characterized by sunken, necrotic lesions appearing after prolonged cold storage, although initial injury occurs much earlier in the storage period. To determine the degree to which the transition to cell death is an active process and sp...

  5. 76 FR 78832 - Protection of Stratospheric Ozone: Listing of Substitutes for Ozone-Depleting Substances...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    ... outside the home. Household freezers offer storage space only at freezing temperatures. Products with both... systems, including cold storage cases, designed to chill food or keep it at a cold temperature for... Administration NOAEL--no observable adverse effect level NPRM--notice of proposed rulemaking NTTAA--National...

  6. Effect of prestorage UV-A, -B, and -C radiation on fruit quality and anthocyanin of 'Duke' blueberries during cold storage.

    PubMed

    Nguyen, Chau T T; Kim, Jeongyun; Yoo, Kil Sun; Lim, Sooyeon; Lee, Eun Jin

    2014-12-17

    Ultraviolet (UV)-A, -B, and -C were radiated to full-ripe blueberries (cv. 'Duke'), and their effects on fruit qualities and phytonutrients during subsequent cold storage were investigated. The blueberries were exposed to each UV light at 6 kJ/m(2) and then stored at 0 °C for 28 days. Weight loss and decay of the fruits after UV treatment were significantly decreased during the cold storage. The total phenolics and antioxidant activities of blueberries after UV-B and -C treatments were always higher than those of the control and UV-A treatment. Individual anthocyanins were markedly increased during the 3 h after the UV-B and -C treatments. The correlation matrix between total phenolics, anthocyanins, and antioxidant activity measured by the 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) assay indicated a significantly close correlation with the individual anthocyanin contents. It was confirmed that the prestorage treatments of UV-B and -C increased the storability and phytochemical accumulation of the full-ripe 'Duke' blueberries during cold storage.

  7. Effect of Caesalpinia sappan L. extract on physico-chemical properties of emulsion-type pork sausage during cold storage.

    PubMed

    Jin, Sang-Keun; Ha, So-Ra; Choi, Jung-Seok

    2015-12-01

    This study was performed to investigate the effect of extract from heart wood of Caesalpinia sappan on the physico-chemical properties and to find the appropriate addition level in the emulsion-type pork sausage during cold storage. The pH of treatments with C. sappan extract was significantly lower than control and T1 during cold storage periods (P<0.05). Also, the reduction of moisture content, and the increase of cooking loss significantly occurred by the addition of 0.2% C. sappan extract. Also, the texture properties and sensory of sausages containing C. sappan extract were decreased compared to control. Inclusion of the C. sappan extract in sausages resulted in lower lightness and higher yellowness, chroma and hue values. However, the antioxidant, antimicrobial activity, and volatile basic nitrogen in the emulsion-type pork sausages with C. sappan extract showed increased quality characteristics during cold storage. In conclusion, the proper addition level of C. sappan extract was 0.1% on the processing of emulsion-type pork sausage. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. On the Method of Efficient Ice Cold Energy Storage Using a Heat Transfer of Direct Contact Phase Change and a Natural Circulation of a Working Medium in an Enclosure

    NASA Astrophysics Data System (ADS)

    Utaka, Yoshio; Saito, Akio; Nakata, Naoki

    The objectives of this report are to propose a new method of the high performance cold energy storage using ice as a phase change material and to clarify the heat transfer characteristics of the apparatus of ice cold energy storage based on the proposed principle. A working medium vapor layer a water layer and a working medium liquid layer stratified in this order from the top were kept in an enclosure composed of a condenser, an evaporator and a condensate receiver-and-return tube. The direct contact heat transfers between water or ice and a working medium in an enclosure were applied for realizing the high performance cold energy storage and release. In the storage and release processes, water changes the phase between the liquid and the solid, and the working medium cnanges between the vapor and the liquid with a natural circulation. Experimental apparatus was manufactured and R12 and R114 were selected as working media in the thermal energy storage enclosure. It was confirmed by the measurements that the efficient formation and melting of ice were achieved. Then, th e heat transfer characteristics were clarified for the effects of the initial water height, the initial height of woking medium liquid layer and the inlet coolant temperature.

  9. Vacuum infiltration of putrescine enhances bioactive compounds and maintains quality of blood orange during cold storage.

    PubMed

    Habibi, Fariborz; Ramezanian, Asghar

    2017-07-15

    The effects of putrescine (Put) treatment on anthocyanin concentrations and other bioactive compounds of two blood orange ('Moro' and 'Tarocco') cultivars during cold storage have been investigated. Put at 0, 1 and 2mM were applied to fruit by vacuum infiltration at 26.665kPa for 8min and then stored at 5°C, and 90% RH for 60days, plus a simulated shelf life of 2days at 20°C. Put treatment maintained higher fruit firmness and reduced weight loss during storage. Anthocyanin, total phenolic content (TPC), ascorbic acid content, and antioxidant activity were also higher in treated fruit than the control during storage. pH and titratable acidity (TA) were highest in treated fruit, while soluble solids concentration (SSC) and SSC/TA ratios were highest in untreated fruit. Overall, the quality of blood oranges maintained by Put treatment during cold storage. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. The ability of a cold-adapted Rhodotorula mucilaginosa strain from Tibet to control blue mold in pear fruit.

    PubMed

    Hu, Hao; Yan, Fujie; Wilson, Charles; Shen, Qing; Zheng, Xiaodong

    2015-12-01

    Cold-adapted yeasts were isolated from soil samples collected in Tibet and evaluated as potential biocontrol agents against blue mold (Penicillium expansum) of pear fruit in cold storage. YC1, an isolate identified as Rhodotorula mucilaginosa, was found to exhibit the greatest biocontrol activity among the different isolates that were screened. A washed cell suspension of YC1 exhibited the best biocontrol activity among three different preparations that were used in the current study. A concentration of 10(8) cells/ml reduced the incidence of decay to 35 %, compared to the control where decay incidence was 100 %. A higher intracellular level of trehalose and a higher proportion of polyunsaturated acids present in YC1, was associated with increased the tolerance of this strain to low temperatures, relative to the other strains that were evaluated. The increased tolerance to low temperature allowed the YC1 strain of yeast to more effectively compete for nutrients and space in wounded pear fruit that had been inoculated with spores of P. expansum and placed in cold storage. The present study demonstrated the ability to select cold-adapted yeasts from cold climates and use them as biocontrol agents of postharvest diseases of fruit placed in cold storage.

  11. Efficacy of lactoferricin B in controlling ready-to-eat vegetable spoilage caused by Pseudomonas spp.

    PubMed

    Federico, Baruzzi; Pinto, Loris; Quintieri, Laura; Carito, Antonia; Calabrese, Nicola; Caputo, Leonardo

    2015-12-23

    The microbial content of plant tissues has been reported to cause the spoilage of ca. 30% of chlorine-disinfected fresh vegetables during cold storage. The aim of this work was to evaluate the efficacy of antimicrobial peptides in controlling microbial vegetable spoilage under cold storage conditions. A total of 48 bacterial isolates were collected from ready-to-eat (RTE) vegetables and identified as belonging to Acinetobacter calcoaceticus, Aeromonas media, Pseudomonas cichorii, Pseudomonas fluorescens, Pseudomonas jessenii, Pseudomonas koreensis, Pseudomonas putida, Pseudomonas simiae and Pseudomonas viridiflava species. Reddish or brownish pigmentation was found when Pseudomonas strains were inoculated in wounds on leaves of Iceberg and Trocadero lettuce and escarole chicory throughout cold storage. Bovine lactoferrin (BLF) and its hydrolysates (LFHs) produced by pepsin, papain and rennin, were assayed in vitro against four Pseudomonas spp. strains selected for their heavy spoiling ability. As the pepsin-LFH showed the strongest antimicrobial effect, subsequent experiments were carried out using the peptide lactoferricin B (LfcinB), well known to be responsible for its antimicrobial activity. LfcinB significantly reduced (P ≤ 0.05) spoilage by a mean of 36% caused by three out of four inoculated spoiler pseudomonads on RTE lettuce leaves after six days of cold storage. The reduction in the extent of spoilage was unrelated to viable cell density in the inoculated wounds. This is the first paper providing direct evidence regarding the application of an antimicrobial peptide to control microbial spoilage affecting RTE leafy vegetables during cold storage.

  12. 7 CFR 305.6 - Cold treatment requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... must be treated after arrival only in cold storage warehouses approved by the Administrator and located... ensure the security and integrity of cold treatment data. The devices must be able to record the date...

  13. Ex-vivo perfusion of donor hearts for human heart transplantation (PROCEED II): a prospective, open-label, multicentre, randomised non-inferiority trial.

    PubMed

    Ardehali, Abbas; Esmailian, Fardad; Deng, Mario; Soltesz, Edward; Hsich, Eileen; Naka, Yoshifumi; Mancini, Donna; Camacho, Margarita; Zucker, Mark; Leprince, Pascal; Padera, Robert; Kobashigawa, Jon

    2015-06-27

    The Organ Care System is the only clinical platform for ex-vivo perfusion of human donor hearts. The system preserves the donor heart in a warm beating state during transport from the donor hospital to the recipient hospital. We aimed to assess the clinical outcomes of the Organ Care System compared with standard cold storage of human donor hearts for transplantation. We did this prospective, open-label, multicentre, randomised non-inferiority trial at ten heart-transplant centres in the USA and Europe. Eligible heart-transplant candidates (aged >18 years) were randomly assigned (1:1) to receive donor hearts preserved with either the Organ Care System or standard cold storage. Participants, investigators, and medical staff were not masked to group assignment. The primary endpoint was 30 day patient and graft survival, with a 10% non-inferiority margin. We did analyses in the intention-to-treat, as-treated, and per-protocol populations. This trial is registered with ClinicalTrials.gov, number NCT00855712. Between June 29, 2010, and Sept 16, 2013, we randomly assigned 130 patients to the Organ Care System group (n=67) or the standard cold storage group (n=63). 30 day patient and graft survival rates were 94% (n=63) in the Organ Care System group and 97% (n=61) in the standard cold storage group (difference 2·8%, one-sided 95% upper confidence bound 8·8; p=0·45). Eight (13%) patients in the Organ Care System group and nine (14%) patients in the standard cold storage group had cardiac-related serious adverse events. Heart transplantation using donor hearts adequately preserved with the Organ Care System or with standard cold storage yield similar short-term clinical outcomes. The metabolic assessment capability of the Organ Care System needs further study. TransMedics. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Suppression of the vacuolar invertase gene prevents cold-induced sweetening in potato

    USDA-ARS?s Scientific Manuscript database

    Storing potato (Solanum tuberosum) tubers at cold temperatures prevents sprouting and minimizes losses due to disease. Unfortunately, cold storage triggers an accumulation of reducing sugars, a phenomenon referred to as cold-induced sweetening (CIS). High-temperature processing of potato tubers wit...

  15. Integrating Geohydrological Models In ATES-Systems Control

    NASA Astrophysics Data System (ADS)

    Bloemendal, Martin

    2015-04-01

    1) Purpose. Accomplish optimal and sustainable use of subsurface for Aquifer Thermal Energy Storage (ATES). 2) Scope. A heat pump in combination with an ATES system can efficiently and sustainably provide heating and cooling for user comfort within buildings. ATES systems are popular in moderate climate in which ATES systems are exploited as they are able to save primary energy. While storing warm and cold groundwater, ATES systems occupy a significant amount of the subsurface space, making that the space in the aquifers below cities is becoming scarce [1]. With the rapid growth of the number of ATES systems, the use of the subsurface intensifies, which raises additional questions regarding its sustainability and the long term profitability of the individual systems. In practice considerable difficulties regarding A) the performance of these installations and B) optimal and sustainable use of the subsurface are met. 3) Approach. Recently it was confirmed [2] that ATES systems can be placed closer to each other with limited effect on their energy efficiency. By placing them closer together we introduce the risk of a tragedy of the commons [3]. Therefore it is of importance to know where the warm and cold zones are over time and enable ATES-controllers to use the subsurface optimal and sustainably. From the field of multi agent systems and complex adaptive systems we use approaches and techniques to make an operation and control system that enables to adapt their control not only based on current demand, but also on current aquifer status and expected future demand. We are developing a numerical groundwater model structure which is fed with operational data of different ATES-systems. While doing this we run into challenges and opportunities like; spatial and temporal scale issues, sustaining the storage with balancing thermal storage and extraction at area level, dynamics and relation between hydrological and thermal influence and consequences for spreading of contaminants, using thermal energy storage for "peak-shaving" of wind/solar power production etc.. I will address the following two topics; - Balancing of stored heating and cooling capacity. To sustain an ATES-system heating and cooling capacity storage must more or less balance. Buildings often do not have a similar heating and cooling demand. Placing ATES-well closer to each other offers the opportunity to exchange energy between different buildings in the subsurface to balance heating and cooling capacity. To be able to do so, thorough understanding of the interaction between thermal influence area resulting from highly dynamic and uncertain energy demand from buildings is required. - The hydrological influence area of ATES wells is much bigger than the thermal influence area. Placing wells closer to each other therefor has a significant effect on the mixing of water and spreading of contaminants (which are often present in shallow aquifers under (old) city centers). We use both analytical and numerical approaches to gain insight in patterns of thermal and contaminant spreading and to find solutions in managing these effects. 4) Results and conclusions The subsurface is of crucial importance for intended energy savings. A control system working towards a global optimum for both the subsurface and buildings, instead of a local optimum for an individual building and local ATES will increase the overall efficiency. What is needed for that is insight in the spatial temperature distribution in the subsurface, in combination with adaptive and robust operational rules. We want to prove that a groundwater model simulating active ATES-systems can provide insight in the subsurface temperature distribution to adjust their control strategy in accordance with up-to-date information. Step by step we are solving the problems on this path, I would like to share and discuss my results, solutions and challenges. References [1] Bloemendal, M., Olsthoorn, T., Boons, F., How to achieve optimal and sustainable use of the subsurface for Aquifer Thermal Energy Storage, Energy Policy 66(2014) 104-114 [2] Sommer, W., Valstar, J., Leusbrock, I., Grotenhuis, T., Rijnaarts, H., Optimization and spatial pattern of large-scale aquifer thermal energy storage, Applied Energy 137 (2015) 322-337 [3] Hardin, G., The tragedy of the commons, Science162 (168) 12-13.

  16. Randomized Trial of Machine Perfusion Versus Cold Storage in Recipients of Deceased Donor Kidney Transplants With High Incidence of Delayed Graft Function.

    PubMed

    Tedesco-Silva, Helio; Mello Offerni, Juliano Chrystian; Ayres Carneiro, Vanessa; Ivani de Paula, Mayara; Neto, Elias David; Brambate Carvalhinho Lemos, Francine; Requião Moura, Lúcio Roberto; Pacheco E Silva Filho, Alvaro; de Morais Cunha, Mirian de Fátima; Francisco da Silva, Erica; Miorin, Luiz Antonio; Demetrio, Daniela Priscila; Luconi, Paulo Sérgio; da Silva Luconi, Waldere Tania; Bobbio, Savina Adriana; Kuschnaroff, Liz Milstein; Noronha, Irene Lourdes; Braga, Sibele Lessa; Barsante, Renata Cristina; Mendes Moreira, João Cezar; Fernandes-Charpiot, Ida Maria Maximina; Abbud-Filho, Mario; Modelli de Andrade, Luis Gustavo; Dalsoglio Garcia, Paula; Tanajura Santamaria Saber, Luciana; Fernandes Laurindo, Alan; Chocair, Pedro Renato; Cuvello Neto, Américo Lourenço; Zanocco, Juliana Aparecida; Duboc de Almeida Soares Filho, Antonio Jose; Ferreira Aguiar, Wilson; Medina Pestana, Jose

    2017-05-01

    This study compared the use of static cold storage versus continuous hypothermic machine perfusion in a cohort of kidney transplant recipients at high risk for delayed graft function (DGF). In this national, multicenter, and controlled trial, 80 pairs of kidneys recovered from brain-dead deceased donors were randomized to cold storage or machine perfusion, transplanted, and followed up for 12 months. The primary endpoint was the incidence of DGF. Secondary endpoints included the duration of DGF, hospital stay, primary nonfunction, estimated glomerular filtration rate, acute rejection, and allograft and patient survivals. Mean cold ischemia time was high but not different between the 2 groups (25.6 ± 6.6 hours vs 25.05 ± 6.3 hours, 0.937). The incidence of DGF was lower in the machine perfusion compared with cold storage group (61% vs. 45%, P = 0.031). Machine perfusion was independently associated with a reduced risk of DGF (odds ratio, 0.49; 95% confidence interval, 0.26-0.95). Mean estimated glomerular filtration rate tended to be higher at day 28 (40.6 ± 19.9 mL/min per 1.73 m 2 vs 49.0 ± 26.9 mL/min per 1.73 m 2 ; P = 0.262) and 1 year (48.3 ± 19.8 mL/min per 1.73 m 2 vs 54.4 ± 28.6 mL/min per 1.73 m 2 ; P = 0.201) in the machine perfusion group. No differences in the incidence of acute rejection, primary nonfunction (0% vs 2.5%), graft loss (7.5% vs 10%), or death (8.8% vs 6.3%) were observed. In this cohort of recipients of deceased donor kidneys with high mean cold ischemia time and high incidence of DGF, the use of continuous machine perfusion was associated with a reduced risk of DGF compared with the traditional cold storage preservation method.

  17. Improving vaccination cold chain in the general practice setting.

    PubMed

    Page, Sue L; Earnest, Arul; Birden, Hudson; Deaker, Rachelle; Clark, Chris

    2008-10-01

    This study compared temperature control in different types of vaccine storing refrigerators in general practice and tested knowledge of general practice staff in vaccine storage requirements. Temperature data loggers were set to serially record the temperature within vaccine refrigerators in 28 general practices, recording at 12 minute intervals over a period of 10 days on each occasion. A survey of vaccine storage knowledge and records of divisions of general practice immunisation contacts were also obtained. There was a significant relationship between type of refrigerator and optimal temperature, with the odds ratio for bar style refrigerator being 0.005 (95% CI: 0.001-0.044) compared to the purpose built vaccine refrigerators. Score on a survey of vaccine storage was also positively associated with optimal storage temperature. General practices that invest in purpose built vaccine refrigerators will achieve standards of vaccine cold chain maintenance significantly more reliably than can be achieved through regular cold chain monitoring and practice supports.

  18. Roasting pumpkin seeds and changes in the composition and oxidative stability of cold-pressed oils.

    PubMed

    Raczyk, Marianna; Siger, Aleksander; Radziejewska-Kubzdela, Elżbieta; Ratusz, Katarzyna; Rudzińska, Magdalena

    2017-01-01

    Pumpkin seed oil is valuable oil for its distinctive taste and aroma, as well as supposed health- promoting properties. The aim of this study was to investigate how roasting pumpkin seeds influences the physicochemical properties of cold-pressed oils. The fatty acid composition, content of phytosterols, carotenoids and tocopherols, oxidative stability and colour were determined in oils after cold pressing and storage for 3 months using GC-FID, GCxGC-ToFMS, HPLC, Rancimat and spectrophotometric methods. The results of this study indicate that the seed-roasting and storage process have no effect on the fatty acid composition of pumpkin seed oils, but does affect phytosterols and tocopherols. The carotenoid content decreased after storage. The colour of the roasted oil was darker and changed significantly during storage. Pumpkin oil obtained from roasted seeds shows better physicochemical properties and oxidative stability than oil from unroasted seeds.

  19. Cold Climate and Retrofit Applications for Air-to-Air Heat Pumps

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Baxter, Van D

    2015-01-01

    Air source heat pumps (ASHP) including air-to-air ASHPs are easily applied to buildings almost anywhere for new construction as well as retrofits or renovations. They are widespread in milder climate regions but their use in cold regions is hampered due to low heating efficiency and capacity at cold outdoor temperatures. Retrofitting air-to-air ASHPs to existing buildings is relatively easy if the building already has an air distribution system. For buildings without such systems alternative approaches are necessary. Examples are ductless, minisplit heat pumps or central heat pumps coupled to small diameter, high velocity (SDHV) air distribution systems. This article presentsmore » two subjects: 1) a summary of R&D investigations aimed at improving the cold weather performance of ASHPs, and 2) a brief discussion of building retrofit options using air-to-air ASHP systems.« less

  20. Differential expression of the enzymes associated with cold-induced sweetening in long term cold stored potatoes

    USDA-ARS?s Scientific Manuscript database

    Accumulation of high levels of reducing sugars during cold storage (4-6°C) known as cold-induced sweetening (CIS) is a major post-harvest disorder and is one of the most significant concerns for the potato processing industry. The biochemical process of reducing sugar accumulation during cold stora...

  1. Present-day monitoring underestimates the risk of exposure to pathogenic bacteria from cold water storage tanks

    PubMed Central

    2018-01-01

    Water-borne bacteria, found in cold water storage tanks, are causative agents for various human infections and diseases including Legionnaires’ disease. Consequently, regular microbiological monitoring of tank water is undertaken as part of the regulatory framework used to control pathogenic bacteria. A key assumption is that a small volume of water taken from under the ball valve (where there is easy access to the stored water) will be representative of the entire tank. To test the reliability of this measure, domestic water samples taken from different locations of selected tanks in London properties between November 2015 and July 2016 were analysed for TVCs, Pseudomonas and Legionella at an accredited laboratory, according to regulatory requirements. Out of ~6000 tanks surveyed, only 15 were selected based on the ability to take a water sample from the normal sampling hatch (located above the ball valve) and from the far end of the tank (usually requiring disassembly of the tank lid with risk of structural damage), and permission being granted by the site manager to undertake the additional investigation and sampling. Despite seasonal differences in water temperature, we found 100% compliance at the ball valve end. In contrast, 40% of the tanks exceeded the regulatory threshold for temperature at the far end of the tank in the summer months. Consequently, 20% of the tanks surveyed failed to trigger appropriate regulatory action based on microbiological analyses of the water sample taken under the ball valve compared to the far end sample using present-day standards. These data show that typical water samples collected for routine monitoring may often underestimate the microbiological status of the water entering the building, thereby increasing the risk of exposure to water bourne pathogens with potential public health implications. We propose that water storage tanks should be redesigned to allow access to the far end of tanks for routine monitoring purposes, and that water samples used to ascertain the regulatory compliance of stored water in tanks should be taken at the point at which water is abstracted for use in the building. PMID:29649274

  2. Application of Cold Storage for Raja Sere Banana (Musa acuminata colla)

    NASA Astrophysics Data System (ADS)

    Crismas, S. R. S.; Purwanto, Y. A.; Sutrisno

    2018-05-01

    Raja Sere is one of the indigenous banana cultivars in Indonesia. This cultivar has a yellow color when ripen, small size and sweet taste. Traditionally, the growers market this banana cultivar to the market without any treatment to delay the ripening process. Banana fruits are commonly being harvested at the condition of hard green mature. At this condition of hard green mature, banana fruits can be stored for a long-term period. The objective of this study was to examine the effect of cold storage on the quality of raja sere banana that stored at 13°C. Banana fruits cultivar Raja Sere were harvested from local farmer field at the condition of hard green mature (about 14 weeks age after the flower bloom). Fifteen bunches of banana were stored in cold storage with a temperature of 13°C for 0, 3, 6, 9, and 12 days, respectively. For the control, room temperature storage (28°C) was used. At a storage period, samples of banana fruits ripened in the ripening chamber by injecting 100 ppm of ethylene gas at 25°C for 24 hours. The quality parameters namely respiration rate, hardness, total soluble solids (TSS), change in color, and weight loss were measured. For those banana fruits stored at room temperature, the shelf-life of banana was only reached up to 6 days. For those banana fruits stored in cold storage, the condition of banana fruits was reached up to 12 days. After cold storage and ripening, the third day measurement was the optimal time for bananas to be consumed which indicated by the yellow color (lightness value = 68.51, a* = 4.74 and value b* = 62.63), TSS 24.30 °Brix and hardness 0.48 kgf, weight loss about 7.53-16.45% and CO2 respiration rate of 100.37 mLCO2 / kg.hr.

  3. 10. CONTEXT VIEW LOOKING SOUTHEAST SHOWING BUILDING 342 (STORAGE MAGAZINE) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. CONTEXT VIEW LOOKING SOUTHEAST SHOWING BUILDING 342 (STORAGE MAGAZINE) ON LEFT AND BUILDING 343 (STORAGE MAGAZINE) ON RIGHT IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  4. 13. CONTEXT VIEW LOOKING EAST SHOWING BUILDING 343 (STORAGE MAGAZINE) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. CONTEXT VIEW LOOKING EAST SHOWING BUILDING 343 (STORAGE MAGAZINE) ON RIGHT AND BUILDING 342 (STORAGE MAGAZINE) ON LEFT IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  5. Genetic and epigenetic stability of cryopreserved and cold-stored hops (Humulus lupulus L.).

    PubMed

    Peredo, Elena L; Arroyo-García, Rosa; Reed, Barbara M; Revilla, M Angeles

    2008-12-01

    Conventional cold storage and cryopreservation methods for hops (Humulus lupulus L.) are available but, to our knowledge, the genetic and epigenetic stability of the recovered plants have not been tested. This study analyzed 51 accessions of hop using the molecular techniques, Random Amplified DNA Polymorphism (RAPD) and Amplified Fragment Length Polymorphism (AFLP), revealing no genetic variation among greenhouse-grown controls and cold stored or cryopreserved plants. Epigenetic stability was evaluated using Methylation Sensitive Amplified Polymorphism (MSAP). Over 36% of the loci were polymorphic when the cold and cryo-treated plants were compared to greenhouse plants. The main changes were demethylation events and they were common to the cryopreserved and cold stored plants indicating the possible effect of the in vitro establishment process, an essential step in both protocols. Protocol-specific methylation patterns were also detected indicating that both methods produced epigenetic changes in plants following cold storage and cryopreservation.

  6. 7 CFR 1170.10 - Storage reporting specifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REPORTING § 1170.10 Storage reporting specifications. (a) Cold Storage Report: (1) Reporting universe: All...) Dairy Products Report: (1) Reporting universe: All manufacturing plants. (2) Products required to be...

  7. 7 CFR 1170.10 - Storage reporting specifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... REPORTING § 1170.10 Storage reporting specifications. (a) Cold Storage Report: (1) Reporting universe: All...) Dairy Products Report: (1) Reporting universe: All manufacturing plants. (2) Products required to be...

  8. 7 CFR 1170.10 - Storage reporting specifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... REPORTING § 1170.10 Storage reporting specifications. (a) Cold Storage Report: (1) Reporting universe: All...) Dairy Products Report: (1) Reporting universe: All manufacturing plants. (2) Products required to be...

  9. 7 CFR 1170.10 - Storage reporting specifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... REPORTING § 1170.10 Storage reporting specifications. (a) Cold Storage Report: (1) Reporting universe: All...) Dairy Products Report: (1) Reporting universe: All manufacturing plants. (2) Products required to be...

  10. 7 CFR 1170.10 - Storage reporting specifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REPORTING § 1170.10 Storage reporting specifications. (a) Cold Storage Report: (1) Reporting universe: All...) Dairy Products Report: (1) Reporting universe: All manufacturing plants. (2) Products required to be...

  11. Changes in carbohydrate content in zucchini fruit (Cucurbita pepo L.) under low temperature stress.

    PubMed

    Palma, Francisco; Carvajal, Fátima; Lluch, Carmen; Jamilena, Manuel; Garrido, Dolores

    2014-03-01

    The postharvest handling of zucchini fruit includes low-temperature storage, making cold stress unavoidable. We have investigated the changes of soluble carbohydrates under this stress and its relation with weight loss and chilling injury in zucchini fruit during postharvest storage at 4 °C and 20 °C for up to 14 days. Two varieties with different degrees of chilling tolerance were compared: Natura, the more tolerant variety, and Sinatra, the variety that suffered more severe chilling-injury symptoms and weight loss. In both varieties, total soluble carbohydrates, reducing soluble carbohydrates and polyols content was generally higher during storage at 4 °C than at 20 °C, thus these parameters are related to the physiological response of zucchini fruit to cold stress. However, the raffinose content increased in Natura and Sinatra fruits during storage at 4 °C and 20 °C, although at 20 °C the increase in raffinose was more remarkable than at 4 °C in both varieties, so that the role of raffinose could be more likely related to dehydration than to chilling susceptibility of zucchini fruit. Glucose, fructose, pinitol, and acid invertase activity registered opposite trends in both varieties against chilling, increasing in Natura and decreasing in Sinatra. The increase in acid invertase activity in Natura fruit during cold storage could contribute in part to the increase of these reducing sugars, whose metabolism could be involved in the adaptation to postharvest cold storage. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  12. Hydrogen Sulfide Protects Renal Grafts Against Prolonged Cold Ischemia-Reperfusion Injury via Specific Mitochondrial Actions.

    PubMed

    Lobb, I; Jiang, J; Lian, D; Liu, W; Haig, A; Saha, M N; Torregrossa, R; Wood, M E; Whiteman, M; Sener, A

    2017-02-01

    Ischemia-reperfusion injury is unavoidably caused by loss and subsequent restoration of blood flow during organ procurement, and prolonged ischemia-reperfusion injury IRI results in increased rates of delayed graft function and early graft loss. The endogenously produced gasotransmitter, hydrogen sulfide (H 2 S), is a novel molecule that mitigates hypoxic tissue injury. The current study investigates the protective mitochondrial effects of H 2 S during in vivo cold storage and subsequent renal transplantation (RTx) and in vitro cold hypoxic renal injury. Donor allografts from Brown Norway rats treated with University of Wisconsin (UW) solution + H 2 S (150 μM NaSH) during prolonged (24-h) cold (4°C) storage exhibited significantly (p < 0.05) decreased acute necrotic/apoptotic injury and significantly (p < 0.05) improved function and recipient Lewis rat survival compared to UW solution alone. Treatment of rat kidney epithelial cells (NRK-52E) with the mitochondrial-targeted H 2 S donor, AP39, during in vitro cold hypoxic injury improved the protective capacity of H 2 S >1000-fold compared to similar levels of the nonspecific H 2 S donor, GYY4137 and also improved syngraft function and survival following prolonged cold storage compared to UW solution. H 2 S treatment mitigates cold IRI-associated renal injury via mitochondrial actions and could represent a novel therapeutic strategy to minimize the detrimental clinical outcomes of prolonged cold IRI during RTx. © 2016 The American Society of Transplantation and the American Society of Transplant Surgeons.

  13. 1. Historic American Buildings Survey Nathaniel R. Ewan, Photographer May ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey Nathaniel R. Ewan, Photographer May 19, 1936 EXTERIOR - SOUTHEAST ELEVATION - Cold Spring Presbyterian Church, West side Seashore Road, Cold Spring, Cape May County, NJ

  14. 2. Historic American Buildings Survey Nathaniel R. Ewan, Photographer May ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey Nathaniel R. Ewan, Photographer May 19, 1936 INTERIOR - CHANCEL DETAIL - Cold Spring Presbyterian Church, West side Seashore Road, Cold Spring, Cape May County, NJ

  15. Assessment of temperatures in the vaccine cold chain in two provinces in Lao People's Democratic Republic: a cross-sectional pilot study.

    PubMed

    Kitamura, Tomomi; Bouakhasith, Viraneth; Phounphenghack, Kongxay; Pathammavong, Chansay; Xeuatvongsa, Anonh; Norizuki, Masataro; Okabayashi, Hironori; Mori, Yoshio; Machida, Munehito; Hachiya, Masahiko

    2018-04-27

    All childhood vaccines, except the oral polio vaccine, should be kept at 2-8 °C, since the vaccine potency can be damaged by heat or freezing temperature. A temperature monitoring study conducted in 2008-2009 reported challenges in cold chain management from the provincial level downwards. The present cross-sectional pilot study aimed to assess the current status of the cold chain in two provinces (Saravan and Xayabouly) of Lao People's Democratic Republic between March-April 2016. Two types of temperature data loggers recorded the temperatures and the proportions of time exposed to < 0 or > 8 °C were calculated. The temperature remained within the appropriate range in the central and provincial storages. However, the vaccines were frequently exposed to > 8 °C in Saravan and < 0 °C in Xayabouly in the district storage. Vaccines were exposed to > 8 °C during the transportation in Saravan and to both > 8 and < 0 °C in Xayabouly. Thus, challenges in managing the cold chain in the district storage and during transportation remain, despite improvements at the provincial storage. A detailed up-to-date nationwide analysis of the current situation of the cold chain is warranted to identify the most appropriate intervention to tackle the remaining challenges.

  16. Quality of Golden papaya stored under controlled atmosphere conditions.

    PubMed

    Martins, Derliane Ribeiro; de Resende, Eder Dutra

    2013-10-01

    This work evaluated physicochemical parameters of Golden papaya stored under refrigeration in controlled atmospheres. The fruits were kept at 13  in chambers containing either 3 or 6% O2 combined with 6%, 10% or 15% CO2. Moreover, a normal atmosphere was produced with 20.8% O2 and 0.03% CO2 with ethylene scrubbing, and a control treatment was used with ambient conditions. Evaluations were performed at the following times: before storage, after 30 days of storage in controlled atmosphere, and after removal from controlled atmosphere and storage for 7 days in the cold room. At the lower O2 levels and higher CO2 levels, the ripening rate was decreased. The drop in pulp acidity was avoided after 30 days of storage at 3% O2, but the fruits reached normal acidity after removal from controlled atmosphere and storage for 7 days in the cold room. The reducing sugars remained at a higher concentration after 30 days under 3% O2 and 15% CO2 even 7 days after removal from controlled atmosphere and storage in the cold room. This atmosphere also preserved the content of ascorbic acid at a higher level.

  17. SECURITY GATE FOR WEAPONS STORAGE AREA. FROM LEFT TO RIGHT, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SECURITY GATE FOR WEAPONS STORAGE AREA. FROM LEFT TO RIGHT, STORAGE BUILDING (BUILDING 3584), CONVENTIONAL MUNITIONS SHOP (BUILDING 3580), AND SECURITY POLICE ENTRY CONTROL BUILDING (BUILDING 3582). VIEW TO SOUTHWEST - Plattsburgh Air Force Base, U.S. Route 9, Plattsburgh, Clinton County, NY

  18. Lamoka, a variety with excellent chip color out of cold storage and resistance to the golden potato cyst nematode

    USDA-ARS?s Scientific Manuscript database

    Lamoka is a white-skinned, white-fleshed potato cultivar variety notable for excellent chip color from cold storage, good yield, and resistance to both common scab and race Ro1 of the golden potato cyst nematode (Globodera rostochiensis). It was selected from a cross made at Cornell University in 1...

  19. Portal Venous Oxygen Persufflation of the Donation after Cardiac Death pancreas in a rat model is superior to static cold storage and hypothermic machine perfusion.

    PubMed

    Reddy, Mettu S; Carter, Noel; Cunningham, Anne; Shaw, James; Talbot, David

    2014-06-01

    Success of clinical pancreatic islet transplantation depends on the mass of viable islets transplanted and the proportion of transplanted islets that survive early ischaemia reperfusion injury. Novel pancreas preservation techniques to improve islet preservation and viability can increase the utilization of donation after cardiac death donor pancreases for islet transplantation. Rat pancreases were retrieved after 30 min of warm ischaemia and preserved by static cold storage, hypothermic machine perfusion or retrograde portal venous oxygen persufflation for 6 h. They underwent collagenase digestion and density gradient separation to isolate islets. The yield, viability, morphology were compared. In vitro function of isolated islets was compared using glucose stimulated insulin secretion test. Portal venous oxygen persufflation improved the islet yield, viability and morphology as compared to static cold storage. The percentage of pancreases with good in vitro function (stimulation index > 1.0) was also higher after oxygen persufflation as compared to static cold storage. Retrograde portal venous oxygen persufflation of donation after cardiac death donor rat pancreases has the potential to improve islet yield. © 2014 Steunstichting ESOT.

  20. Effects of Growth Temperature and Postharvest Cooling on Anthocyanin Profiles in Juvenile and Mature Brassica oleracea.

    PubMed

    Socquet-Juglard, Didier; Bennett, Alexandra A; Manns, David C; Mansfield, Anna Katharine; Robbins, Rebecca J; Collins, Thomas M; Griffiths, Phillip D

    2016-02-24

    The effects of growth temperatures on anthocyanin content and profile were tested on juvenile cabbage and kale plants. The effects of cold storage time were evaluated on both juvenile and mature plants. The anthocyanin content in juvenile plants ranged from 3.82 mg of cyanidin-3,5-diglucoside equivalent (Cy equiv)/g of dry matter (dm) at 25 °C to 10.00 mg of Cy equiv/g of dm at 16 °C, with up to 76% diacylated anthocyanins. Cold storage of juvenile plants decreased the total amount of anthocyanins but increased the diacylated anthocyanin content by 3-5%. In mature plants, cold storage reduced the total anthocyanin content from 22 to 12.23 mg/g after 5 weeks of storage in red cabbage, while the total anthocyanin content increased after 2 weeks of storage from 2.34 to 3.66 mg of Cy equiv/g of dm in kale without having any effect on acylation in either morphotype. The results obtained in this study will be useful for optimizing anthocyanin production.

  1. Changes in Cuticular Wax Composition of Two Blueberry Cultivars during Fruit Ripening and Postharvest Cold Storage.

    PubMed

    Chu, Wenjing; Gao, Haiyan; Chen, Hangjun; Wu, Weijie; Fang, Xiangjun

    2018-03-21

    Cuticular wax plays an important role for the quality of blueberry fruits. In this study, the cuticular wax composition of two blueberry cultivars, 'Legacy' ( Vaccinium corymbosum) and 'Brightwell' ( Vaccinium ashei), was examined during fruit ripening and postharvest cold storage. The results showed that wax was gradually deposited on the epidermis of blueberry fruits and the content of major wax compounds, except that for diketones, increased significantly during fruit ripening. The total wax content was 2-fold greater in 'Brightwell' blueberries than that in 'Legacy' blueberries during fruit ripening. The total wax content of both cultivars decreased during 30 days of storage at 4 °C, and the variation of cuticular wax composition was cultivar-dependent. The content of diketones decreased significantly in 'Legacy' blueberries, while the content of triterpenoids and aliphatic compounds showed different fold changes in 'Brightwell' blueberries after 30 days of storage at 4 °C. Overall, our study provided a quantitative and qualitative overview of cuticular wax compounds of blueberry fruits during ripening and postharvest cold storage.

  2. Method and apparatus for operating an improved thermocline storage unit

    DOEpatents

    Copeland, R.J.

    1982-09-30

    A method and apparatus for operating a thermocline storage unit in which an insulated barrier member is provided substantially at the interface region between the hot and cold liquids in the storage tank. The barrier member physically and thermally separates the hot and cold liquids substantially preventing any diffusing or mixing between them and substantially preventing any heat transfer there between. The barrier member follows the rise and fall of the interface region between the liquids as the tank is charged and discharged. Two methods of maintaining it in the interface region are disclosed. With the structure and operation of the present invention and in particular the significant reduction in diffusing or mixing between the hot and cold liquids as well as the significant reduction in the thermal heat transfer between them, the performance of the storage tank is improved. More specifically, the stability of the interface region or thermocline is enhanced and the thickness of the thermocline is reduced producing a corresponding increase in the steepness of the temperature gradient across the thermocline and a more efficiently operating thermocline storage unit.

  3. Method and apparatus for operating an improved thermocline storage unit

    DOEpatents

    Copeland, Robert J.

    1985-01-01

    A method and apparatus for operating a thermocline storage unit in which an insulated barrier member is provided substantially at the interface region between the hot and cold liquids in the storage tank. The barrier member physically and thermally separates the hot and cold liquids substantially preventing any diffusing or mixing between them and substantially preventing any heat transfer therebetween. The barrier member follows the rise and fall of the interface region between the liquids as the tank is charged and discharged. Two methods of maintaining it in the interface region are disclosed. With the structure and operation of the present invention and in particular the significant reduction in diffusing or mixing between the hot and cold liquids as well as the significant reduction in the thermal heat transfer between them, the performance of the storage tank is improved. More specifically, the stability of the interface region or thermocline is enhanced and the thickness of the thermocline is reduced producing a corresponding increase in the steepness of the temperature gradient across the thermocline and a more efficiently operating thermocline storage unit.

  4. 2. WATER TREATMENT PUMPING AND STORAGE BUILDING, REAR AND RIGHT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. WATER TREATMENT PUMPING AND STORAGE BUILDING, REAR AND RIGHT SIDES, LOOKING SOUTHWEST. - NIKE Missile Base SL-40, Water Treatment & Storage Building, Southern portion of launch area, southeast of Ready Building, Hecker, Monroe County, IL

  5. 1. WATER TREATMENT PUMPING AND STORAGE BUILDING, FRONT AND LEFT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. WATER TREATMENT PUMPING AND STORAGE BUILDING, FRONT AND LEFT SIDES, LOOKING NORTHEAST. - NIKE Missile Base SL-40, Water Treatment & Storage Building, Southern portion of launch area, southeast of Ready Building, Hecker, Monroe County, IL

  6. Coherent and dynamic beam splitting based on light storage in cold atoms

    PubMed Central

    Park, Kwang-Kyoon; Zhao, Tian-Ming; Lee, Jong-Chan; Chough, Young-Tak; Kim, Yoon-Ho

    2016-01-01

    We demonstrate a coherent and dynamic beam splitter based on light storage in cold atoms. An input weak laser pulse is first stored in a cold atom ensemble via electromagnetically-induced transparency (EIT). A set of counter-propagating control fields, applied at a later time, retrieves the stored pulse into two output spatial modes. The high visibility interference between the two output pulses clearly demonstrates that the beam splitting process is coherent. Furthermore, by manipulating the control lasers, it is possible to dynamically control the storage time, the power splitting ratio, the relative phase, and the optical frequencies of the output pulses. With further improvements, the active beam splitter demonstrated in this work might have applications in photonic photonic quantum information and in all-optical information processing. PMID:27677457

  7. Storage and sterilization techniques: the specific role of the cold chain.

    PubMed

    Guinebault, A

    1986-01-01

    Focus in this discussion is on reasons for the cold chain, management of vaccine supplies (regional storage, peripheral centers, and the clinic), the facilities and their use, monitoring the cold chain, and training participants in the cold chain. To remain active, vaccines must be maintained continuously within a specific temperature range from the moment they are produced until they are injected. This is the meaning of the cold chain. If the cold chain is broken at any point, the vaccines must be destroyed for they will have lost their effectiveness. To function properly, a cold chain requires the combined presence of efficient, reliable equipment, and of qualified, vigilant personnel at all levels. The cold chain is composed of the following elements: a national storage center, near an international airport, with a 1-year supply of vaccines for the entire target population; regional storage centers with a 3-month supply of vaccines for the entire population of the region; peripheral immunization centers scattered throughout the region, managing a supply for about 1 month; clinics, which either perform vaccinations on the spot and/or supply mobile teams, depending on the strategy; and mobile teams, with portable cold boxes, with an autonomy of several days. The main problems occur at the local levels, and more specifically with respect to transportation and the fuel and power supplies, as well as cold packs. At the central level, the 1-year supply of vaccines generally is stored in cold rooms. Personnel in charge of central strorage also are responsible for transportation to and from these cold rooms. Once the space required for storing vaccines is determined, the facilities required at each level may be evaluated. The information essential to the choice must be considered in each case. The main criteria involved are outlined. There are many devices for monitoring the function of the cold chain: indicators, which accompany the vaccines from the central depot to the peripheral centers show any excesses in temperature and their duration; and devices such as thermometers show the present temperature, independently of the "history" of the vaccine. Some devices are available for checking individual elements of the cold chain from time to time. The World Health Organization (WHO) has developed a training strategy aimed at people on all levels: international consultants and decisionmakers involved in programming the Expanded Program on Immunization; technicians in charge of maintenance; and medical personnel.

  8. Effect of tea polyphenols on lipid peroxidation and antioxidant activity of litchi (Litchi chinensis Sonn.) fruit during cold storage.

    PubMed

    Chen, Wenrong; Zhang, Zhenzhen; Shen, Yanwen; Duan, Xuewu; Jiang, Yuemin

    2014-10-20

    To understand the potential of application of tea polyphenols to the shelf life extension and quality maintenance of litchi (Litchi chinensis Sonn.) fruit, the fruits were dipped into a solution of 1% tea phenols for 5 min before cold storage at 4 °C. Changes in browning index, contents of anthocyanins and phenolic compounds, superoxide dismutase (SOD) and peroxidase (POD) activities, O2.- production rate and H2O2 content, levels of relative leakage rate and lipid peroxidation, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were measured after 0, 10, 20 and 30 days of cold storage. The results showed that application of tea polyphenols markedly delayed pericarp browning, alleviated the decreases in contents of total soluble solids (TSS) and ascorbic acid, and maintained relatively high levels of total phenolics and anthocyanins of litchi fruit after 30 days of cold storage. Meanwhile, the treatment reduced the increases in relative leakage rate and lipid peroxidation content, delayed the increases in both O2.- production rate and H2O2 contents, and increased SOD activity but reduced POD activity throughout this storage period. These data indicated that the delayed pericarp browning of litchi fruit by the treatment with tea polyphenols could be due to enhanced antioxidant capability, reduced accumulations of reactive oxygen species and lipid peroxidation, and improved membrane integrity.

  9. 1. EXTERIOR VIEW OF BUILDING 25A (COLD CHAMBER), LOOKING NORTHEAST, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. EXTERIOR VIEW OF BUILDING 25A (COLD CHAMBER), LOOKING NORTHEAST, WITH WIND TUNNEL IN BACKGROUND (1991). - Wright-Patterson Air Force Base, Area B, Buildings 25 & 24,10-foot & 20-foot Wind Tunnel Complex, Northeast side of block bounded by K, G, Third, & Fifth Streets, Dayton, Montgomery County, OH

  10. 30 CFR 57.4531 - Surface flammable or combustible liquid storage buildings or rooms.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Surface flammable or combustible liquid storage... flammable or combustible liquid storage buildings or rooms. (a) Surface storage buildings or storage rooms in which flammable or combustible liquids, including grease, are stored and that are within 100 feet...

  11. 30 CFR 57.4531 - Surface flammable or combustible liquid storage buildings or rooms.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Surface flammable or combustible liquid storage... flammable or combustible liquid storage buildings or rooms. (a) Surface storage buildings or storage rooms in which flammable or combustible liquids, including grease, are stored and that are within 100 feet...

  12. 30 CFR 57.4531 - Surface flammable or combustible liquid storage buildings or rooms.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Surface flammable or combustible liquid storage... flammable or combustible liquid storage buildings or rooms. (a) Surface storage buildings or storage rooms in which flammable or combustible liquids, including grease, are stored and that are within 100 feet...

  13. 30 CFR 57.4531 - Surface flammable or combustible liquid storage buildings or rooms.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Surface flammable or combustible liquid storage... flammable or combustible liquid storage buildings or rooms. (a) Surface storage buildings or storage rooms in which flammable or combustible liquids, including grease, are stored and that are within 100 feet...

  14. Building America Best Practices Series Volume 12: Builders Challenge Guide to 40% Whole-House Energy Savings in the Cold and Very Cold Climates

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Baechler, Michael C.; Gilbride, Theresa L.; Hefty, Marye G.

    2011-02-01

    This best practices guide is the twelfth in a series of guides for builders produced by PNNL for the U.S. Department of Energy’s Building America program. This guide book is a resource to help builders design and construct homes that are among the most energy-efficient available, while addressing issues such as building durability, indoor air quality, and occupant health, safety, and comfort. With the measures described in this guide, builders in the cold and very cold climates can build homes that have whole-house energy savings of 40% over the Building America benchmark with no added overall costs for consumers. Themore » best practices described in this document are based on the results of research and demonstration projects conducted by Building America’s research teams. Building America brings together the nation’s leading building scientists with over 300 production builders to develop, test, and apply innovative, energy-efficient construction practices. Building America builders have found they can build homes that meet these aggressive energy-efficiency goals at no net increased costs to the homeowners. Currently, Building America homes achieve energy savings of 40% greater than the Building America benchmark home (a home built to mid-1990s building practices roughly equivalent to the 1993 Model Energy Code). The recommendations in this document meet or exceed the requirements of the 2009 IECC and 2009 IRC and thos erequirements are highlighted in the text. This document will be distributed via the DOE Building America website: www.buildingamerica.gov.« less

  15. Influence of cold storage prior to and after ripening on quality factors and sensory attributes of ‘Hass’ avocados

    USDA-ARS?s Scientific Manuscript database

    Partially-ripened avocados are often held in cold storage in an attempt to enable the consistent delivery of ripe fruit to food service or retail outlets, although the effect on the quality of such fruit is incompletely understood. ‘Hass’ avocados were ripened to near ripeness (13.3 - 17.8 N) at 20 ...

  16. Neural network model for survival and growth of Salmonella 8,20:-:z6 in ground chicken thigh meat during cold storage: extrapolation to other serotypes

    USDA-ARS?s Scientific Manuscript database

    Mathematical models that predict behavior of human bacterial pathogens in food are valuable tools for assessing and managing this risk to public health. A study was undertaken to develop a model for predicting behavior of Salmonella 8,20:-:z6 in chicken meat during cold storage and to determine how...

  17. Effect of pullulan on the water distribution, microstructure and textural properties of rice starch gels during cold storage.

    PubMed

    Chen, Long; Tian, Yaoqi; Tong, Qunyi; Zhang, Zipei; Jin, Zhengyu

    2017-01-01

    The effects of pullulan on the water distribution, microstructure and textural properties of rice starch gels during cold storage were investigated by low field-nuclear magnetic resonance (LF-NMR), scanning electron microscope (SEM), and texture profile analysis (TPA). The addition of pullulan reduced the transversal relaxation time of rice starch gels during cold storage. The microstructure of rice starch gel with 0.5% pullulan was denser and more uniform compared with that of rice starch without pullulan in each period of storage time. With regard to textural properties, 0.01% pullulan addition did not significantly change the texture of rice starch gels, while 0.5% pullulan addition appeared to reduce the hardness and retain the springiness of rice starch gels (P⩽0.05). The restriction effects of pullulan on water mobility and starch retrogradation were hypothesized to be mainly responsible for the water retention, gel structure maintenance, and modification of the textural attributes of rice starch gels. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Effects of thinned young apple polyphenols on the quality of grass carp (Ctenopharyngodon idellus) surimi during cold storage.

    PubMed

    Sun, Lijun; Sun, Jiaojiao; Thavaraj, Pridhuvi; Yang, Xingbin; Guo, Yurong

    2017-06-01

    The aim of this study was to investigate the effects of young apple polyphenols (YAP) on the quality of grass cap surimi (GCS) during storage at 4°C. The addition of YAP into GCS was found to be effective in delaying lipid oxidation, soluble myofibrillar protein (SMP) degradation and changes of L ∗ , a ∗ and b ∗ values of GCS. Chlorogenic acid was screened to be the primary component showing preservative effects. YAP was shown to protect the functional properties of SMP during cold storage, retarding both the decrease in emulsifying activity and stability, and the increase in surface hydrophobicity of SMP. Additionally, the loss of gel strength and texture of GCS with YAP were significantly (P<0.05) lower than that of GCS without YAP during cold storage. Therefore, YAP may be developed as a natural antioxidant to maintain the quality and to extend the shelf life of freshwater fish surimi. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Sugar metabolism, chip color, invertase activity, and gene expression during long-term cold storage of potato (Solanum tuberosum) tubers from wild-type and vacuolar invertase silencing lines of Katahdin.

    PubMed

    Wiberley-Bradford, Amy E; Busse, James S; Jiang, Jiming; Bethke, Paul C

    2014-11-16

    Storing potato tubers at low temperatures minimizes sprouting and disease but can cause an accumulation of reducing sugars in a process called cold-induced sweetening. Tubers with increased amounts of reducing sugars produce dark-colored, bitter-tasting fried products with elevated amounts of acrylamide, a possible carcinogen. Vacuolar invertase (VInv), which converts sucrose produced by starch breakdown to glucose and fructose, is the key determinant of reducing sugar accumulation during cold-induced sweetening. In this study, wild-type tubers and tubers in which VInv expression was reduced by RNA interference were used to investigate time- and temperature-dependent changes in sugar contents, chip color, and expression of VInv and other genes involved in starch metabolism in tubers during long-term cold storage. VInv activities and tuber reducing sugar contents were much lower, and tuber sucrose contents were much higher, in transgenic than in wild-type tubers stored at 3-9°C for up to eight months. Large differences in VInv mRNA accumulation were not observed at later times in storage, especially at temperatures below 9°C, so differences in invertase activity were likely established early in the storage period and maintained by stability of the invertase protein. Sugar contents, chip color, and expression of several of the studied genes, including AGPase and GBSS, were affected by storage temperature in both wild-type and transgenic tubers. Though transcript accumulation for other sugar-metabolism genes was affected by storage temperature and duration, it was essentially unaffected by invertase silencing and altered sugar contents. Differences in stem- and bud-end sugar contents in wild-type and transgenic tubers suggested different compartmentalization of sucrose at the two ends of stored tubers. VInv silencing significantly reduced cold-induced sweetening in stored potato tubers, likely by means of differential VInv expression early in storage. Transgenic tubers retained sensitivity to storage temperature, and accumulated greater amounts of sucrose, glucose and fructose at 3°C than at 7-9°C. At each storage temperature, suppression of VInv expression and large differences in tuber sugar contents had no effect on expression of AGPase and GBSS, genes involved in starch metabolism, suggesting that transcription of these genes is not regulated by tuber sugar content.

  20. Assessment of the influence of different sample processing and cold storage duration on plant free proline content analyses.

    PubMed

    Teklić, Tihana; Spoljarević, Marija; Stanisavljević, Aleksandar; Lisjak, Miroslav; Vinković, Tomislav; Parađiković, Nada; Andrić, Luka; Hancock, John T

    2010-01-01

    A method which is widely accepted for the analysis of free proline content in plant tissues is based on the use of 3% sulfosalicylic acid as an extractant, followed by spectrophotometric quantification of a proline-ninhydrin complex in toluene. However, sample preparation and storage may influence the proline actually measured. This may give misleading or difficult to compare data. To evaluate free proline levels fresh and frozen strawberry (Fragaria × ananassa Duch.) leaves and soybean [Glycine max (L.) Merr.] hypocotyl tissues were used. These were ground with or without liquid nitrogen and proline extracted with sulfosalicylic acid. A particular focus was the influence of plant sample cold storage duration (1, 4 and 12 weeks at -20°C) on tissue proline levels measured. The free proline content analyses, carried out in leaves of Fragaria × ananassa Duch. as well as in hypocotyls of Glycine max (L.) Merr., showed a significant influence of the sample preparation method and cold storage period. Long-term storage of up to 12 weeks at -20°C led to a significant increase in the measured proline in all samples analysed. The observed changes in proline content in plant tissue samples stored at -20°C indicate the likelihood of the over-estimation of the proline content if the proline analyses are delayed. Plant sample processing and cold storage duration seem to have an important influence on results of proline analyses. Therefore it is recommended that samples should be ground fresh and analysed immediately. Copyright © 2010 John Wiley & Sons, Ltd.

  1. A Chitosan Coating Containing Essential Oil from Origanum vulgare L. to Control Postharvest Mold Infections and Keep the Quality of Cherry Tomato Fruit

    PubMed Central

    Barreto, Tainá A.; Andrade, Sonalle C. A.; Maciel, Janeeyre F.; Arcanjo, Narciza M. O.; Madruga, Marta S.; Meireles, Bruno; Cordeiro, Ângela M. T.; Souza, Evandro L.; Magnani, Marciane

    2016-01-01

    The efficacy of an edible chitosan coating (CHI; 4 mg/mL) and Origanum vulgare L. essential oil (OVEO; 1.25 μL/mL) for maintaining the quality of cherry tomato fruit during storage at room (25°C; 12 days) and cold (12°C; 24 days) temperatures was assessed. CHI and OVEO in combination showed in vitro fungicidal effects against R. stolonifer and Aspergillus niger. CHI-OVEO coating reduced the incidence of black mold and soft rot caused by these fungi in artificially contaminated cherry tomato fruit during storage at both temperatures. CHI-OVEO coating delayed the appearance of the first visible signs of black mold and soft rot in artificially contaminated cherry tomato fruit stored at room temperature by 6 days and by more than 9 days in those stored at cold temperature. At the end of storage at room and cold temperature fruit coated with CHI-OVEO showed higher firmness (>2 N/mm) and lower weight loss (>2%) compared to uncoated tomato fruit. CHI-OVEO coating delayed the decrease of lycopene, ascorbic citric acid, glucose and fructose during the storage time assessed at room or cold temperatures. The increase of catechin, myricetin, caffeic and syringic acids was higher (1–9 mg/g) in cherry tomato fruit coated with CHI-OVEO compared to uncoated fruit during the storage at both temperatures studied. CHI-OVEO coating is a feasible treatment for maintaining the storage quality of cherry tomato fruit. PMID:27877156

  2. Effect of cold storage on larval and adult Anastrepha ludens (Diptera: Tephritidae) viability in commercially ripe, artificially infested Persea americana 'Hass'.

    PubMed

    Aluja, M; Díaz-Fleischer, F; Arredondo, J; Valle-Mora, J; Rull, J

    2010-12-01

    Commercially ripe 'Hass' avocados, Persea americana Mill, artificially exposed to wild Anastrepha ludens (Loew) (Diptera: Tephritidae) females 24 h after harvest were placed in a cold storage facility to determine the effect of low temperature on larval survival and adult viability. Fruit were left for 3, 6, 9, and 12 d in a cold room at 5 degrees C followed by a 20-25-d period at ambient temperature to allow for larval development and pupation. Hass avocados and grapefruit, Citrus paradisi Macfadyen, maintained at ambient temperature served as controls. Overall, only 0.23% of the Hass avocados and 19.30% of the grapefruit were infested. The number of infested fruit increased with decreasing exposure time to cold. Puparia from cold-treated Hass avocados were significantly smaller than those stemming from cold-treated grapefruit. Hass avocados exposed for 12 d to 5 degrees C yielded no puparia, and those exposed for 6 and 9 d yielded 22 and two puparia, respectively, but no adults. Although Hass avocados exposed to cold temperature for 3 d yielded adults that reached sexual maturity (N = 16), females laid inviable eggs. Grapefruit exposed to cold for 12 d yielded normal-sized puparia (but no adults), whereas those exposed over 9 d yielded females able to lay viable eggs. We conclude that exposing fruit to cold storage after packing and during transport represents an effective risk-mitigating procedure in the highly improbable event that a gravid A. ludens female might lay eggs in a commercially ripe Hass avocado that had been left unprotected in a packinghouse.

  3. Experimental study on the cool storage performance of super absorbent polymers for cool storage clothes

    NASA Astrophysics Data System (ADS)

    Li, Shidong; Mo, Caisong; Wang, Junze; Zheng, Jingfu; Tian, Ruhong

    2017-11-01

    In this paper, a kind of cool storage clothes which can cool the human body in high temperature condition is put forward. super absorbent polymers was selected as a cold storage material, through at the normal and extreme environment simulation, the cold storage materials were prepared with different composition, and their performance was tested. Test results show that:under normal temperature conditions, the 1:50 concentration of super absorbent polymers continued to release the longest cooling time, compared with pure water, cooling time extended 43 minutes by about 30%; under the condition of 37°C, the 1:100 concentration of super absorbent polymers continued to release the longest cooling time, compared with pure water, cooling time extended 105 minutes by about 50%.

  4. Fate of Listeria monocytogenes on Fresh Apples under Different Storage Temperatures.

    PubMed

    Sheng, Lina; Edwards, Katheryn; Tsai, Hsieh-Chin; Hanrahan, Ines; Zhu, Mei-Jun

    2017-01-01

    Fresh apples are typically stored for up to 1 year commercially; different apple varieties require different storage temperatures to maintain their quality characteristics. There is sparse information available about Listeria monocytogenes survival on fresh apples under various storage temperatures. The objective of this study was to comprehensively evaluate the effect of storage temperature on apple fruit decay and L. monocytogenes survival. Unwaxed apple fruits of selected varieties (Fuji and Granny Smith) were dip inoculated in a three-strain L. monocytogenes cocktail to establish ∼3.5 and 6.0 Log 10 CFU/apple. Twenty-four hours post-inoculation, apples were subjected to 1, 4, 10, or 22°C storage for up to 3 months. Apples under the different storage treatments were sampled at 1-, 4-, 7- and 14-day for short-term storage under all four tested temperatures, and 2-, 4-, 8-, and 12-week for long-term storage at 1, 4, and 10°C. A set of uninoculated and unwaxed apples were simultaneously subjected to the previously mentioned storage temperatures and sampled biweekly for their total bacterial count (TPC) and yeasts/molds (Y/M) count. During the 2-week short-term storage, L. monocytogenes population on organic Granny Smith apples stored at 1, 4, or 10°C was reduced by 0.2-0.3 Log. When apples were stored at 22°C, there was a 0.5-1.2 Log 10 CFU/apple reduction 14-day post storage dependent on the initial inoculation level. During the 12-week cold storage under 1, 4, and 10°C, L. monocytogenes count on organic Granny Smith apples decreased by 0.5-1.5 Log 10 CFU/apple for both inoculation levels. L. monocytogenes had similar survival pattern on conventional Granny Smith and Fuji apples with 0.8-2.0 Log 10 CFU/apple reduction over a 3-month cold storage period. Interestingly, both TPC and Y/M count were stable regardless of apple variety or cultivation practice during the 12-week storage at all tested temperatures. In summary, while L. monocytogenes did not proliferate on apple surfaces during 12 weeks of refrigerated storage, only a limited reduction of L. monocytogenes was observed in this study. Therefore, the apple industry cannot rely on cold storage alone to control this pathogen. Additional interventions are needed to eradicate Listeria on fresh apples during long-term cold storage.

  5. 63. CONTEXT VIEW LOOKING WEST OF BUILDING 372 (HAZARDOUS STORAGE) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    63. CONTEXT VIEW LOOKING WEST OF BUILDING 372 (HAZARDOUS STORAGE) IN BASE SPARES AREA WITH BUILDING 370 (ADMINISTRATIVE OFFICE BUILDING) IN BACKGROUND. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  6. Mannose and fructose metabolism in red blood cells during cold storage in SAGM.

    PubMed

    Rolfsson, Óttar; Johannsson, Freyr; Magnusdottir, Manuela; Paglia, Giuseppe; Sigurjonsson, Ólafur E; Bordbar, Aarash; Palsson, Sirus; Brynjólfsson, Sigurður; Guðmundsson, Sveinn; Palsson, Bernhard

    2017-11-01

    Alternate sugar metabolism during red blood cell (RBC) storage is not well understood. Here we report fructose and mannose metabolism in RBCs during cold storage in SAGM and the impact that these monosaccharides have on metabolic biomarkers of RBC storage lesion. RBCs were stored in SAGM containing uniformly labeled 13 C-fructose or 13 C-mannose at 9 or 18 mmol/L concentration for 25 days. RBCs and media were sampled at 14 time points during storage and analyzed using ultraperformance liquid chromatography-mass spectrometry. Blood banking quality assurance measurements were performed. Red blood cells incorporated fructose and mannose during cold storage in the presence of glucose. Mannose was metabolized in preference to glucose via glycolysis. Fructose lowered adenosine triphosphate (ATP) levels and contributed little to ATP maintenance when added to SAGM. Both monosaccharides form the advanced glycation end product glycerate. Mannose activates enzymes in the RBC that take part in glycan synthesis. Fructose or mannose addition to RBC SAGM concentrates may not offset the shift in metabolism of RBCs that occurs after 10 days of storage. Fructose and mannose metabolism at 4°C in SAGM reflects their metabolism at physiologic temperature. Glycerate excretion is a measure of protein deglycosylation activity in stored RBCs. No cytoprotective effect was observed upon the addition of either fructose or mannose to SAGM. © 2017 AABB.

  7. Fungicides improve field performance of stored loblolly and longleaf pine seedlings

    Treesearch

    John C. Brissette

    1996-01-01

    Seedlings of loblolly and longleaf pine lifted in December, January, and February were treated with either benomyl or ridomil before cold storage. Along with an untreated control, they were planted after cold storage of less than 1 wk, 3 wk, and 6 wk. Survival was measured in mid-June after planting, and after 1 and 4 yr in the field. Total height was measured after 4...

  8. Managing ‘Bartlett’ pear fruit ripening with 1-methylcyclopropene reapplication during cold storage

    USDA-ARS?s Scientific Manuscript database

    Repeated low-dose 1-MCP-applications were evaluated during cold storage of ‘Bartlett’ pear fruit to overcome long-term ripening inhibition of a high dose 1-MCP treatment at harvest. Fruit were exposed to 1-MCP at 0, 0.42, 4.2 or 42 umol m-3 at harvest in year one, and to 0, 0.42 or 42 umol m-3 in y...

  9. Human Milk Adrenomedullin Is Unstable During Cold Storage at 4°C.

    PubMed

    Peila, Chiara; Coscia, Alessandra; Bertino, Enrico; Li Volti, Giovanni; Galvano, Fabio; Barbagallo, Ignazio; Gazzolo, Diego

    2017-11-01

    Under some circumstances human milk (HM) extraction and refrigerated storage may be necessary. Depending on the length and on the type of cold storage, milk may lose some important properties, but current advices on safe HM storage are discordant. Moreover until now no data in literature were present on the effect of prolonged cold storage on biologically active components of the HM such as adrenomedullin (AM). This important peptide is involved in response to hypoxia and inflammation, associated with neovascularization, in several tissues. The aim is to evaluate: (a) the presence of AM in preterm and term HM and (b) the concentration of AM in refrigerated milk at 4°C at 24-hour intervals, up to 96 hours of storage. The experiment was repeated four times. Immediately after collection, each HM sample deriving from each mother was divided into two parts as follows: "Pool" line and "Single Mother" line. One part (Pool line) was pooled and then divided into five aliquots. The other part (Single Mother line) was divided into five aliquots. From each line, one aliquot was analyzed within 3 hours, while the others were stored in the refrigerator for 24, 48, 72, and 96 hours, respectively, and then analyzed. AM levels were determined using a specific ELISA test. AM was detectable in all samples. Its concentration was significantly higher in preterm milk with respect to term milk (p < 0.05). Significant differences were observed during the cold storage: the AM levels decreased steadily during the storage and the remaining concentration at 96 hours is ∼2%. This study provides evidences regarding the presence of AM in HM, regardless of the gestational age. In particular, the refrigeration of fresh HM in controlled conditions significantly affected its bioactivity and nutritional quality related with AM, already at 24 hours.

  10. 1. VIEW OF PATTERN STORAGE BUILDING NO. 5 (wooden structure ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW OF PATTERN STORAGE BUILDING NO. 5 (wooden structure to right) AND NO. 6 (brick structure to the left, ca. 1891) ON THE EASTERN BANK OF THE STONY CREEK RIVER. Brick foundation for the pump machinery and brick conduit tunnel are still intact in the basement of Pattern Storage Building No. 6. - Johnson Steel Street Rail Company, Pattern Storage Building, 525 Central Avenue, Johnstown, Cambria County, PA

  11. Multi Canister Overpack (MCO) Topical Report [SEC 1 THRU 3

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    LORENZ, B.D.

    In February 1995, the US Department of Energy (DOE) approved the Spent Nuclear Fuel (SNF) Project's ''Path Forward'' recommendation for resolution of the safety and environmental concerns associated with the deteriorating SNF stored in the Hanford Site's K Basins (Hansen 1995). The recommendation included an aggressive series of projects to design, construct, and operate systems and facilitates to permit the safe retrieval, packaging, transport, conditions, and interim storage of the K Basins' SNF. The facilities are the Cold VAcuum Drying Facility (CVDF) in the 100 K Area of the Hanford Site and the Canister Storage building (CSB) in the 200more » East Area. The K Basins' SNF is to be cleaned, repackaged in multi-canister overpacks (MCOs), removed from the K Basins, and transported to the CVDF for initial drying. The MCOs would then be moved to the CSB and weld sealed (Loscoe 1996) for interim storage (about 40 years). One of the major tasks associated with the initial Path Forward activities is the development and maintenance of the safety documentation. In addition to meeting the construction needs for new structures, the safety documentation for each must be generated.« less

  12. 12. NORTHWEST CORNER OF STORAGE MAGAZINE (BUILDING 342) IN STORAGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. NORTHWEST CORNER OF STORAGE MAGAZINE (BUILDING 342) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  13. Credit BG. View looking northeast (42°) at storage building used ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Credit BG. View looking northeast (42°) at storage building used to store equipment near southeast edge of aircraft apron in the vicinity of Building 4305 (Unicon Portable Hangar) - Edwards Air Force Base, North Base, Equipment Storage Building, East of Second Street, Boron, Kern County, CA

  14. VIEW OF CEMETERY SECTIONS C AND D, WITH LODGE BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF CEMETERY SECTIONS C AND D, WITH LODGE BUILDING AT LEFT BACKGROUND AND NEW YORK (8TH NY ARTILLERY) MONUMENT AT RIGHT BACKGROUND. VIEW TO SOUTHEAST. - Cold Harbor National Cemetery, 6038 Cold Harbor Road, Mechanicsville, Hanover County, VA

  15. 4. PHOTOCOPY, ARCHITECTURAL DETAILS FOR WATER TREATMENT PUMPING AND STORAGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. PHOTOCOPY, ARCHITECTURAL DETAILS FOR WATER TREATMENT PUMPING AND STORAGE BUILDING. - NIKE Missile Base SL-40, Water Treatment & Storage Building, Southern portion of launch area, southeast of Ready Building, Hecker, Monroe County, IL

  16. View from east to west of PAR site storage building; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View from east to west of PAR site storage building; formerly PAR dispensary - Stanley R. Mickelsen Safeguard Complex, Storage Building, Across street from Family Housing Units 110 & 111, Nekoma, Cavalier County, ND

  17. Seasonal changes of DNA fragmentation and quality of raw and cold-stored stallion spermatozoa.

    PubMed

    Wach-Gygax, L; Burger, D; Malama, E; Bollwein, H; Fleisch, A; Jeannerat, E; Thomas, S; Schuler, G; Janett, F

    2017-09-01

    In this study annual fluctuations of DNA fragmentation and quality of cold-stored equine sperm were evaluated. Ejaculates were collected weekly during one year from 15 stallions. Ejaculate volume, sperm concentration and total sperm count were determined and semen was then extended and cold-stored for 48 h. Sperm motility was evaluated by CASA before and after 24 as well as 48 h of cold storage. In addition, the percentages of sperm with intact plasma membrane and acrosome (PMAI %) and with low intracellular Ca 2+ level were determined in cold-stored semen (24 h, 48 h). SCSA™ was performed to assess mean DFI, SD of DFI and % DFI in raw frozen-thawed as well as in extended sperm after 24 and 48 h of storage. The month of semen collection affected (P < 0.05) all parameters evaluated in raw semen and all criteria except progressive motility as well as rapid cells in semen stored for 24 and 48 h, respectively. Ejaculate volume was higher and sperm concentration lower in summer compared to winter and motility lower in July than in any other month of the year (P < 0.05). In semen processed in April and stored for 24 h the percentage of rapid cells was improved compared to January and after 48 h of storage progressive motility (%) was higher in January and October than in July (P < 0.05). After 24 h of cold storage PMAI % was higher in October than in January and after 48 h values were higher in September compared to January and February as well as from April to July (P < 0.05). Regarding sperm with low intracellular Ca +2 level (%) after storage for 24 and 48 h, higher values were measured in winter and in October compared to April, June and July (P < 0.01). Seasonal changes in DNA fragmentation were most evident with respect to mean DFI. In raw frozen-thawed semen mean DFI was lower from August to November than in June and July (P < 0.001). Values were lower during winter compared to spring and early summer (P < 0.05) and lower in December than from April to September (P < 0.001). After 24 h of cold storage mean DFI was lower in September and October when compared to January, February, May, July and November (P < 0.05) and after 48 h storage mean DFI was reduced in spring and autumn compared to February, June and July (P < 0.05). In conclusion, a seasonal effect was evident on semen characteristics of raw and cold-stored sperm. Semen quality was impaired in midsummer when low sperm motility and viability were combined with an elevated DNA fragmentation and Ca 2+ level of sperm. Copyright © 2017. Published by Elsevier Inc.

  18. 68. INTERIOR SHOT OF ENTRANCE TO BUILDING 272 (PLUTONIUM STORAGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    68. INTERIOR SHOT OF ENTRANCE TO BUILDING 272 (PLUTONIUM STORAGE BUILDING) LOOKING WEST. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  19. Storage stability and composition changes of three cold-pressed nut oils under refrigeration and room temperature conditions.

    PubMed

    Rabadán, Adrián; Álvarez-Ortí, Manuel; Pardo, José Emilio; Alvarruiz, Andrés

    2018-09-01

    Chemical composition and stability parameters of three cold-pressed nut oils (almond, walnut and pistachio) were monitored for up to 16 months of storage at 5 °C, 10 °C, 20 °C and room temperature. Freshly pressed pistachio oil had lower peroxide value than almond oil and higher induction period than almond and walnut oils, indicating a higher stability. The peroxide values increased faster at room temperature than at lower temperatures during the storage time, and the highest increase was for pistachio oil stored at room temperature exposed to daylight. The induction period decreased for all three nut oils during the storage time, regardless of the storage conditions. Pistachio oil remained the most stable oil at the end of the storage time, followed by almond oil. The percentage of polyunsaturated fatty acids decreased slightly throughout the storage. Copyright © 2018 Elsevier Ltd. All rights reserved.

  20. Effect of 1-methylcyclopropene treatment on green asparagus quality during cold storage

    NASA Astrophysics Data System (ADS)

    Zhang, Peng; Zhang, Min; Wang, Shaojin; Wu, Zhishuang

    2012-10-01

    Green asparagus was treated with 1-methylcyclopropene at three concentration levels at room temperature for 24 h after harvest to evaluate the postharvest quality during cold storage at 4°C. Comparing with the controls, the loss of vitamin C, decomposition of chlorophyll, and accumulation of the malonydiadehyde under treatments of 1-methylcyclopropene were reduced during storage. The enzyme activities in asparagus including peroxidase and phenylalanine ammonia lyase were inhibited by 1-methylcyclopropene treatments, while the activity of superoxide dismutase was enhanced. Based on non-significant difference of the treated samples with 6 ìl l-1, 1-methylcyclopropene treatments at 4 ìl l-1 could be selected to maintain postharvest quality of green asparagus and provide long storage life.

  1. Response to Short-Term Cold Storage for Eggs of Agasicles hygrophila (Coleoptera: Chrysomelidae), a Biological Control Agent of Alligator Weed Alternanthera philoxeroides (Caryophyllales: Amaranthaceae).

    PubMed

    Liu, Yan-Hong; Jia, Dong; Yuan, Xiao-Fang; Wang, Yuan-Xin; Chi, Hsin; Ridsdill-Smith, Thomas James; Ma, Rui-Yan

    2018-05-08

    The alligator weed flea beetle, Agasicles hygrophila Selman & Vogt (Coleoptera: Chrysomelidae) has been used very successfully for the biological control of the widely-distributed invasive weed Alternanthera philoxeroides (Mart.) Griseb (Caryophyllales: Amaranthaceae). In order to extend the 'shelf life' of natural enemies released in biological control programs, cold storage has proven to be a valuable commercial procedure. To determine a suitable low temperature for storage of A. hygrophila, we conducted short-term cold storage treatments of eggs (4°C for 0.5, 1, 2, 5 d, and 7.5, 10, 15°C for 5 d and a control of 25°C; all eggs were returned to 25°C after the treatments). We evaluated the effects of these treatments on the subsequent fitness of the populations based on a demographic analysis using group-reared age-stage two-sex life tables. For 5 d storage, temperatures below 10°C had lethal effects, which were also observed at 4°C for 2 d storage. Storage at 4°C for 0.5 d did not affect the fitness of A. hygrophila, but it did not prolong the developmental time. Storage at 10°C for 5 d significantly decreased rates of population increase compared with 25°C. A. hygrophila stored at 15°C for 5 d had similar age-(stage) specific survival rates, rates of population increase, increased longevity and reproductive capability to the controls at 25°C. It is concluded that there were no significant fitness costs after 5 d storage at 15°C, which is therefore potentially a suitable storage temperature for A. hygrophila eggs.

  2. Cold Helium Pressurization for Liquid Oxygen/Liquid Methane Propulsion Systems: Fully-Integrated Hot-Fire Test Results

    NASA Technical Reports Server (NTRS)

    Morehead, R. L.; Atwell, M. J.; Melcher, J. C.; Hurlbert, E. A.

    2016-01-01

    Hot-fire test demonstrations were successfully conducted using a cold helium pressurization system fully integrated into a liquid oxygen (LOX) / liquid methane (LCH4) propulsion system (Figure 1). Cold helium pressurant storage at near liquid nitrogen (LN2) temperatures (-275 F and colder) and used as a heated tank pressurant provides a substantial density advantage compared to ambient temperature storage. The increased storage density reduces helium pressurant tank size and mass, creating payload increases of 35% for small lunar-lander sized applications. This degree of mass reduction also enables pressure-fed propulsion systems for human-rated Mars ascent vehicle designs. Hot-fire test results from the highly-instrumented test bed will be used to demonstrate system performance and validate integrated models of the helium and propulsion systems. A pressurization performance metric will also be developed as a means to compare different active pressurization schemes.

  3. Prolonged maintenance of 2,3-diphosphoglycerate acid and adenosine triphosphate in red blood cells during storage.

    PubMed

    de Korte, Dirk; Kleine, Mya; Korsten, Herbert G H; Verhoeven, Arthur J

    2008-06-01

    Current additive solutions (ASs) for red cells (RBCs) do not maintain a constant level of critical metabolites such as adenosine triphosphate (ATP) and 2,3-diphosphoglycerate acid (2,3-DPG) during cold storage. From the literature it is known that the intracellular pH is an important determinant of RBC metabolism. Therefore, a new, alkaline, AS was developed with the aim to allow cold storage of RBCs with stable product characteristics. Whole blood-derived RBCs (leukoreduced) were resuspended in experimental medium phosphate-adenine-guanosine-glucose-gluconate-mannitol (PAGGG-M; pH 8.2) with and without washing in the same medium. During cold storage several in vitro variables, such as intracellular pH, 2,3-DPG, ATP, and hemolysis, were analyzed. During cold storage, RBCs resuspended in PAGGG-M showed a constant ATP level (approx. 6 mumol/g Hb) and a very limited hemolysis (<0.2%). The 2,3-DPG content showed an increase until Day 21 (150% of initial level), followed by a slow decrease, with at Day 35 still 100 percent of the initial level. RBCs washed in PAGGG-M even showed a continuous increase of 2,3-DPG during 35 days, with a maximum level of 200 percent of the initial value. The effect of PAGGG-M appears to be related to long-lasting effects of the initial intracellular pH shortly after production. Resuspension of RBCs in our alkaline medium PAGGG-M resulted in a RBC unit of high quality during storage for up to at least 35 days, with 2,3-DPG levels of higher than 10 mumol per g Hb, hemolysis of less than 0.2 percent, and ATP levels of higher than 5 mumol per g Hb.

  4. Differential Metabolic Rearrangements after Cold Storage Are Correlated with Chilling Injury Resistance of Peach Fruits

    PubMed Central

    Bustamante, Claudia A.; Monti, Laura L.; Gabilondo, Julieta; Scossa, Federico; Valentini, Gabriel; Budde, Claudio O.; Lara, María V.; Fernie, Alisdair R.; Drincovich, María F.

    2016-01-01

    Reconfiguration of the metabolome is a key component involved in the acclimation to cold in plants; however, few studies have been devoted to the analysis of the overall metabolite changes after cold storage of fruits prior to consumption. Here, metabolite profiling of six peach varieties with differential susceptibility to develop mealiness, a chilling-injury (CI) symptom, was performed. According to metabolic content at harvest; after cold treatment; and after ripening, either following cold treatment or not; peach fruits clustered in distinct groups, depending on harvest-time, cold treatment, and ripening state. Both common and distinct metabolic responses among the six varieties were found; common changes including dramatic galactinol and raffinose rise; GABA, Asp, and Phe increase; and 2-oxo-glutarate and succinate decrease. Raffinose content after long cold treatment quantitatively correlated to the degree of mealiness resistance of the different peach varieties; and thus, raffinose emerges as a candidate biomarker of this CI disorder. Xylose increase after cold treatment was found only in the susceptible genotypes, indicating a particular cell wall reconfiguration of these varieties while being cold-stored. Overall, results indicate that peach fruit differential metabolic rearrangements due to cold treatment, rather than differential metabolic priming before cold, are better related with CI resistance. The plasticity of peach fruit metabolism renders it possible to induce a diverse metabolite array after cold, which is successful, in some genotypes, to avoid CI. PMID:27746802

  5. Oil-in-water emulsion gels stabilized with chia (Salvia hispanica L.) and cold gelling agents: Technological and infrared spectroscopic characterization.

    PubMed

    Pintado, T; Ruiz-Capillas, C; Jiménez-Colmenero, F; Carmona, P; Herrero, A M

    2015-10-15

    This paper reports on the development of olive oil-in-water emulsion gels containing chia (Salvia hispanica L.) (flour or seed) and cold gelling agents (transglutaminase, alginate or gelatin). The technological and structural characteristics of these emulsion gels were evaluated. Both structural and technological changes in emulsion gels resulting from chilled storage were also determined. The color and texture of emulsion gels depend on both the cold gelling agents used and chilled storage. Lipid oxidation increased (p < 0.05) during storage in emulsion gels containing transglutaminase or alginate. Analyses of the half-bandwidth of the 2923 cm(-1) band and the area of the 3220 cm(-1) band suggest that the order/disorder of the oil lipid chain related to lipid interactions and droplet size in the emulsion gels could be decisive in determining their textural properties. The half-bandwidth of 2923 cm(-1) band and area of 3220 cm(-1) band did not show significant differences during chilled storage. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Fort McClellan, ammunition storage building 4404. Planar view of rear ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Fort McClellan, ammunition storage building 4404. Planar view of rear (west) side, view towards the east northeast without scale - Fort McClellan Ammunition Storage Area, Building No. 4404, Second Avenue (Magazine Road), Anniston, Calhoun County, AL

  7. Survey of Postharvest Quality Characteristics During Long-Term Farmers Stock Storage

    USDA-ARS?s Scientific Manuscript database

    The length of time that peanuts remain in farmers stock storage is variable. With the record harvest of the 2012 peanut crop, some peanuts remained in farmers stock storage for up to 12 months before being shelled and placed in cold storage or shipped to the manufacturer. To investigate potential ...

  8. Storage temperature and 1-MCP treatment affect storage disorders and physiological attributes of ‘Royal Gala’ apples

    USDA-ARS?s Scientific Manuscript database

    ‘Royal Gala’ apples [Malus domestica (Borkh.) Mansf.] can develop postharvest disorders such as flesh browning, senescent breakdown, peeling, cracking, or shriveling during and after cold storage. The objective of this study was to examine the effects of storage temperature and a range of 1-methylc...

  9. 76 FR 78641 - Southwestern Gas Storage Technical Conference; Notice of Public Conference

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    ... Storage Technical Conference; Notice of Public Conference Take notice that on February 16, 2012 at 9 a.m... technical conference with interested parties to discuss issues related to natural gas storage development in... Cold Weather Event of February 1-5, 2011: Causes and Recommendations, ``[a]dditional gas storage...

  10. Changes of the Components of Fresh Seaweed, Undaria pinnatifida, by Different Strage Conditions

    NASA Astrophysics Data System (ADS)

    Onodera, Munenaka; Yoshie-Stark, Yumiko; Suzuki, Takesh

    This study was performed to keep the quality and to prolong the shelf life of fresh Undaria pinnatifida, by different storage conditions. Changes of the contents of chlorophyll a (Chl a) and its derivatives, β-carotene, pH, molecular weight of alginate and molecular weight distribution were determined during the storage of U. pinnatifida. The conditions of cold storage at -3 to 7°C with air or O2, storage in seawater, and storage in slurry ice made of seawater were tested. Chl a and β-carotene contents, and the pH of U. pinnatifida were decreased following the increment of storage days. Significant decrease of Chl a content and molecular weight of U. pinnatifida was detected under cold storage especially at 7°C. The storage by icing in slurry ice and by super chilling at -3°C inhibited the degradation of Chl a and β-carotene of U. pinnatifida. The content of pheophorbide a or pH were recognized as useful factors to evaluate the quality and freshness of U. pinnatifida.

  11. Light storage in a cold atomic ensemble with a high optical depth

    NASA Astrophysics Data System (ADS)

    Park, Kwang-Kyoon; Chough, Young-Tak; Kim, Yoon-Ho

    2017-06-01

    A quantum memory with a high storage efficiency and a long coherence time is an essential element in quantum information applications. Here, we report our recent development of an optical quantum memory with a rubidium-87 cold atom ensemble. By increasing the optical depth of the medium, we have achieved a storage efficiency of 65% and a coherence time of 51 μs for a weak laser pulse. The result of a numerical analysis based on the Maxwell-Bloch equations agrees well with the experimental results. Our result paves the way toward an efficient optical quantum memory and may find applications in photonic quantum information processing.

  12. PLUG STORAGE BUILDING, TRA611, AWAITS SHIELDING SOIL TO BE PLACED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PLUG STORAGE BUILDING, TRA-611, AWAITS SHIELDING SOIL TO BE PLACED OVER PLUG STORAGE TUBES. WING WALLS WILL SUPPORT EARTH FILL. MTR, PROCESS WATER BUILDING, AND WORKING RESERVOIR IN VIEW BEYOND PLUG STORAGE. CAMERA FACES NORTHEAST. INL NEGATIVE NO. 2949. Unknown Photographer, 7/30/1951 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  13. Characterization of biologic response modifiers in the supernatant of conventional, refrigerated, and cryopreserved platelets.

    PubMed

    Johnson, Lacey; Tan, Shereen; Jenkins, Emily; Wood, Ben; Marks, Denese C

    2018-04-01

    Alternatives to room temperature storage of platelets (PLTs) are of interest to support blood banking logistics. The aim of this study was to compare the presence of biologic response modifiers (BRMs) in PLT concentrates stored under conventional room temperature conditions with refrigerated or cryopreserved PLTs. A three-arm pool-and-split study was carried out using buffy coat-derived PLTs stored in 30% plasma/70% SSP+. The three matched treatment arms were as follows: room temperature (20-24°C), cold (2-6°C), and cryopreserved (-80°C with DMSO). Liquid-stored PLTs were tested over a 21-day period, while cryopreserved PLTs were tested immediately after thawing and reconstitution in 30% plasma/70% SSP+ and after storage at room temperature. Coagulation factor activity was comparable between room temperature and cold PLTs, with the exception of protein S, while cryopreserved PLTs had reduced Factor (F)V and FVIII activity. Cold-stored PLTs retained α-granule proteins better than room temperature or cryopreserved PLTs. Cryopreservation resulted in 10-fold higher microparticle generation than cold-stored PLTs, but both groups contained significantly more microparticles than those stored at room temperature. The supernatant from both cold and cryopreserved PLTs initiated faster clot formation and thrombin generation than room temperature PLTs. Cold storage and cryopreservation alter the composition of the soluble fraction of stored PLTs. These differences in coagulation proteins, cytokines, and microparticles likely influence both the hemostatic capacity of the components and the auxiliary functions. © 2017 AABB.

  14. Effects of ZnO nanoparticle-coated packaging film on pork meat quality during cold storage.

    PubMed

    Suo, Biao; Li, Huarong; Wang, Yuexia; Li, Zhen; Pan, Zhili; Ai, Zhilu

    2017-05-01

    There has been limited research on the use of ZnO nanoparticle-coated film for the quality preservation of pork meat under low temperature. In the present study, ZnO nanoparticles were mixed with sodium carboxymethyl cellulose (CMC-Na) to form a nanocomposite film, to investigate the effect of ZnO nanoparticle-coated film on pork meat quality and the growth of bacteria during storage under low temperature. When ZnO nanoparticle-coated film was used as the packaging material for pork meat for 14 days of cold storage at 4 °C, the results demonstrated a significant effect on restricting the increases in total volatile basic nitrogen and pH levels, limiting the decreases of lightness (increased L* value) and redness (increased a* value), and maintaining the water-holding capacity compared to the control pork samples (P < 0.05). The present study also discovered that the ZnO nanoparticle-coated film restrained the increase in total plate count (TPC). When Staphylococcus aureus was used as the representative strain, scanning electron microscopy revealed that ZnO nanoparticles increased the occurrence of cell membrane rupture under cold conditions. ZnO nanoparticle-coated film helps retain the quality of pork meat during cold storage by increasing the occurrence of microorganism injury. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  15. 5. SOUTHEAST CORNER OF BUILDING 260 (STORAGE STRUCTURE A) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. SOUTHEAST CORNER OF BUILDING 260 (STORAGE STRUCTURE A) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  16. 14. WEST FRONT ELEVATION OF BUILDING 343 (STORAGE MAGAZINE) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. WEST FRONT ELEVATION OF BUILDING 343 (STORAGE MAGAZINE) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  17. 11. WEST FRONT ELEVATION OF BUILDING 342 (STORAGE MAGAZINE) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. WEST FRONT ELEVATION OF BUILDING 342 (STORAGE MAGAZINE) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  18. 9. FERTILIZER PLANT AND STORAGE BUILDINGS, LOOKING EAST FROM BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. FERTILIZER PLANT AND STORAGE BUILDINGS, LOOKING EAST FROM BUILDING 149; LIVESTOCK HOLDING BUILDINGS (HOG AND SHEEP HOTELS) OCCUPIED OPEN AREA IN FOREGROUND - Rath Packing Company, Sycamore Street between Elm & Eighteenth Streets, Waterloo, Black Hawk County, IA

  19. Pancreas preservation for pancreas and islet transplantation

    PubMed Central

    Iwanaga, Yasuhiro; Sutherland, David E.R.; Harmon, James V.; Papas, Klearchos K.

    2010-01-01

    Purpose of review To summarize advances and limitations in pancreas procurement and preservation for pancreas and islet transplantation, and review advances in islet protection and preservation. Recent findings Pancreases procured after cardiac death, with in-situ regional organ cooling, have been successfully used for islet transplantation. Colloid-free Celsior and histidine-tryptophan-ketoglutarate preservation solutions are comparable to University of Wisconsin solution when used for cold storage before pancreas transplantation. Colloid-free preservation solutions are inferior to University of Wisconsin solution for pancreas preservation prior to islet isolation and transplantation. Clinical reports on pancreas and islet transplants suggest that the two-layer method may not offer significant benefits over cold storage with the University of Wisconsin solution: improved oxygenation may depend on the graft size; benefits in experimental models may not translate to human organs. Improvements in islet yield and quality occurred from pancreases treated with inhibitors of stress-induced apoptosis during procurement, storage, isolation or culture. Pancreas perfusion may be desirable before islet isolation and transplantation and may improve islet yields and quality. Methods for real-time, noninvasive assessment of pancreas quality during preservation have been implemented and objective islet potency assays have been developed and validated. These innovations should contribute to objective evaluation and establishment of improved pancreas preservation and islet isolation strategies. Summary Cold storage may be adequate for preservation before pancreas transplants, but insufficient when pancreases are processed for islets or when expanded donors are used. Supplementation of cold storage solutions with cytoprotective agents and perfusion may improve pancreas and islet transplant outcomes. PMID:18685343

  20. Yield and cold storage of Trichoderma conidia is influenced by substrate pH and storage temperature.

    PubMed

    Steyaert, Johanna M; Chomic, Anastasia; Nieto-Jacobo, Maria; Mendoza-Mendoza, Artemio; Hay, Amanda J; Braithwaite, Mark; Stewart, Alison

    2017-05-01

    In this study we examined the influence of the ambient pH during morphogenesis on conidial yield of Trichoderma sp. "atroviride B" LU132 and T. hamatum LU593 and storage at low temperatures. The ambient pH of the growth media had a dramatic influence on the level of Trichoderma conidiation and this was dependent on the strain and growth media. On malt-extract agar, LU593 yield decreased with increasing pH (3-6), whereas yield increased with increasing pH for LU132. During solid substrate production the reverse was true for LU132 whereby yield decreased with increasing pH. The germination potential of the conidia decreased significantly over time in cold storage and the rate of decline was a factor of the strain, pH during morphogenesis, growth media, and storage temperature. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Earth Observations taken by the Expedition 17 Crew

    NASA Image and Video Library

    2008-10-01

    ISS017-E-018075 (1 Oct. 2008) --- The Pueblo Chemical Depot in Colorado is featured in this image photographed by an Expedition 17 crewmember on the International Space Station. This view illustrates the unusual man-made landscape of the Pueblo Chemical Depot located near the city of Pueblo, Colorado. The Depot was built during World War II by the U.S. Army to house and ship ammunition needed for war efforts, and this role transitioned to missile repair and maintenance during the Cold War with the Soviet Union. The current use of the Depot is to house chemical munitions, but changes are underway by the U.S. Army Chemical Materials Agency to destroy these munitions and make the site environmentally safe for reuse -- while also protecting the surrounding local environment. The stippled landscape pattern visible from low Earth orbit is due to hundreds of concrete and earth-covered storage "igloos" that form ordered rows across the site (top). It is within these igloos that chemical munitions and other materials are stored. Larger, white roofed maintenance buildings once used for munitions storage were built with separate compartments to minimize potential damage from explosions. Other features visible in this detailed image include linear roadway (light tan) and rail (dark brown) lines, black irregular surface impoundments of water, and various rectangular office and industrial buildings at lower left.

  2. 3. CONTEXT VIEW LOOKING NORTHWEST OF BUILDING 259 (NORTH SENTRY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. CONTEXT VIEW LOOKING NORTHWEST OF BUILDING 259 (NORTH SENTRY POST) IN STORAGE ARE WITH BUILDING 258 (STORAGE IGLOO) IN BACKGROUND. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  3. 8. CONTEXT VIEW LOOKING NORTHWEST OF BUILDING 262 (SOUTH SENTRY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. CONTEXT VIEW LOOKING NORTHWEST OF BUILDING 262 (SOUTH SENTRY POST) IN STORAGE AREA WITH BUILDING 260 (STORAGE STRUCTURE A) AT LEFT. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  4. 78. BUILDINGS NO. 537, GENERAL STORAGE (DRY HOUSE), NO. 538, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    78. BUILDINGS NO. 537, GENERAL STORAGE (DRY HOUSE), NO. 538, GENERAL STORAGE (GRAPHITING & SORTING HOUSE), LOOKING SOUTHEAST AT NORTHWEST AND SOUTHWEST SIDES. BUILDING NO. 537 WAS ONCE USED AS A DRY HOUSE, AND BUILDING NO. 538 AS A GLAZING (GRAPHITING) AND SORTING HOUSE. - Picatinny Arsenal, 500 Area, Powder Factory & Power House, State Route 15 near I-80, Dover, Morris County, NJ

  5. Cold sweetening diversity in Andean potato germplasm from Argentina.

    PubMed

    Colman, Silvana L; Massa, Gabriela A; Carboni, Martín F; Feingold, Sergio E

    2017-11-01

    Cold-induced sweetening (CIS) is the accumulation of sucrose and reducing sugars in potato tubers at low temperatures. This process is central for the potato processing industry. During potato chip and French fry production, reducing sugars participate in the Maillard reaction to produce dark pigmented products not acceptable to consumers. Andean potatoes (Solanum tuberosum Group Andigena) constitute an enormous wealth of potato germplasm that can contribute to increase genetic diversity in breeding programs of many traits, including CIS. We analyzed reducing sugar content and chip quality in freshly harvested and cold-stored tubers from 48 native accessions. Andean accessions showed high variation in reducing sugar content and were classified in three types of CIS responses: type I, reducing sugar content before and after 4°C storage was lower than the value required by industry; type II, reducing sugar content before storage was acceptable, but after 4°C storage incremented up to non-acceptable levels; and type III, reducing sugar content was unacceptable before and after storage. Five Andean accessions presented acceptable reducing sugar content and good chip quality before and after 4°C storage in a consistent manner throughout several experiments. These features make them a useful source for improving the potato industry. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  6. 15. INTERIOR VIEW LOOKING SOUTHWEST OF BUILDING 343 (STORAGE MAGAZINE) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. INTERIOR VIEW LOOKING SOUTHWEST OF BUILDING 343 (STORAGE MAGAZINE) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  7. 4. WEST REAR ELEVATION OF BUILDING 260 (STORAGE STRUCTURE A) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. WEST REAR ELEVATION OF BUILDING 260 (STORAGE STRUCTURE A) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  8. FACILITY LAYOUT OF FUEL STORAGE BUILDING (CPP603) SHOWING STORAGE BASINS, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FACILITY LAYOUT OF FUEL STORAGE BUILDING (CPP-603) SHOWING STORAGE BASINS, FUEL ELEMENT CUTTING FACILITY, AND DRY GRAPHITE STORAGE FACILITY. INL DRAWING NUMBER 200-0603-00-030-056329. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  9. 2. COLD FLOW LABORATORY, VIEW TOWARDS NORTH. Glenn L. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. COLD FLOW LABORATORY, VIEW TOWARDS NORTH. - Glenn L. Martin Company, Titan Missile Test Facilities, Cold Flow Laboratory Building B, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO

  10. 1. COLD FLOW LABORATORY, VIEW TOWARDS EAST. Glenn L. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. COLD FLOW LABORATORY, VIEW TOWARDS EAST. - Glenn L. Martin Company, Titan Missile Test Facilities, Cold Flow Laboratory Building B, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO

  11. Energy Storage Criteria Handbook.

    DTIC Science & Technology

    1982-10-01

    Phase Change Material Heating System .......................... 311 14.3.1 Analysis of Storage Purpose ........................... 312 14.3.2 Choosing...329 Worksheet I: Cost Analysis of PCM System ...................... 330 14.4 Water Tank Cold Storage...Selecting Components ........................333 14.5.6 Economic Analysis .......................................334 Worksheet A: Cooling Load and Tank

  12. Optical storage with electromagnetically induced transparency in cold atoms at a high optical depth

    NASA Astrophysics Data System (ADS)

    Zhang, Shanchao; Zhou, Shuyu; Liu, Chang; Chen, J. F.; Wen, Jianming; Loy, M. M. T.; Wong, G. K. L.; Du, Shengwang

    2012-06-01

    We report experimental demonstration of efficient optical storage with electromagnetically induced transparency (EIT) in a dense cold ^85Rb atomic ensemble trapped in a two-dimensional magneto-optical trap. By varying the optical depth (OD) from 0 to 140, we observe that the optimal storage efficiency for coherent optical pulses has a saturation value of 50% as OD > 50. Our result is consistent with that obtained from hot vapor cell experiments which suggest that a four-wave mixing nonlinear process degrades the EIT storage coherence and efficiency. We apply this EIT quantum memory for narrow-band single photons with controllable waveforms, and obtain an optimal storage efficiency of 49±3% for single-photon wave packets. This is the highest single-photon storage efficiency reported up to today and brings the EIT atomic quantum memory close to practical application because an efficiency of above 50% is necessary to operate the memory within non-cloning regime and beat the classical limit.

  13. N2 Gas Flushing Limits the Rise of Antibiotic-Resistant Bacteria in Bovine Raw Milk during Cold Storage

    PubMed Central

    Munsch-Alatossava, Patricia; Jääskeläinen, Susanna; Alatossava, Tapani; Gauchi, Jean-Pierrre

    2017-01-01

    Antibiotic resistance has been noted to be a major and increasing human health issue. Cold storage of raw milk promotes the thriving of psychrotrophic/psychrotolerant bacteria, which are well known for their ability to produce enzymes that are frequently heat stable. However, these bacteria also carry antibiotic resistance (AR) features. In places, where no cold chain facilities are available and despite existing recommendations numerous adulterants, including antibiotics, are added to raw milk. Previously, N2 gas flushing showed real potential for hindering bacterial growth in raw milk at a storage temperature ranging from 6 to 25°C. Here, the ability of N2 gas (N) to tackle antibiotic- resistant bacteria was tested and compared to that of the activated lactoperoxidase system (HT) for three raw milk samples that were stored at 6°C for 7 days. To that end, the mesophiles and psychrotrophs that were resistant to gentamycin (G), ceftazidime (Ce), levofloxacin (L), and trimethoprim-sulfamethoxazole (TS) were enumerated. For the log10 ratio (which is defined as the bacterial counts from a certain condition divided by the counts on the corresponding control), classical Analyses of Variance (ANOVA) was performed, followed by a mean comparison with the Ryan-Einot-Gabriel-Welsch multiple range test (REGWQ). If the storage “time” factor was the major determinant of the recorded effects, cold storage alone or in combination with HT or with N promoted a sample-dependent response in consideration of the AR levels. The efficiency of N in limiting the increase in AR was highest for fresh raw milk and was judged to be equivalent to that of HT for one sample and superior to that of HT for the two other samples; moreover, compared to HT, N seemed to favor a more diverse community at 6°C that was less heavily loaded with antibiotic multi-resistance features. Our results imply that N2 gas flushing could strengthen cold storage of raw milk by tackling the bacterial spoilage potential while simultaneously hindering the increase of bacteria carrying antibiotic resistance/multi-resistance features. PMID:28469611

  14. Desert architecture for educational buildings, a case study: A center for training university graduates

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ebeid, M.

    1996-10-01

    A new program for training graduates in desert development is being implemented by the Desert Development Center (DDC) of the American University in Cairo. The facilities consist of fifty bed/sitting rooms for accommodating 100 students. Each unit consists of two rooms and a bathroom for the use of 4 students; a lecture theater which can house 120 students, with adjoining office for trainers as well as necessary facilities; a general cafeteria which can serve 120--150 persons and an adjoining dining room for teaching staff. The cafeteria building also houses the kitchen; a cold storage area; a laundry room, storerooms, sleepingmore » quarters and services for the labor force of the building complex; a system of solar water heaters; and a special sanitary sewage system for treatment of waste water produced by the building`s activities. When designing and implementing this complex, architectural elements and building philosophy based on the concept of integrating with the environment were considered. Elements included orientation heights and building materials suited to the desert environment, thick walls, outer and inner finishing materials, roofs, malkafs, floors, colors, solar heaters, lighting, green areas, windbreaks, terraces, and furniture. The paper includes a general evaluation of this educational building based on the PRA approach (Participatory Rapid Appraisal) involving those living and working in it. As a result of her position with the project, the author was able to evaluate the original designs, recommend modifications, and evaluate their implementation and fulfillment of the original goals of the projects.« less

  15. Real time-based targeted Metabolomic approach as risk assessment tools for controlling superficial scald in ‘Granny Smith’ apples during storage

    USDA-ARS?s Scientific Manuscript database

    The risk of superficial scald incidence in ‘Granny Smith’ apple [Malus sylvestris (L.) Mill var. domestica (Borkh.) Mansf.] fruit during cold or controlled atmosphere (CA) storage increases with increased storage duration and oxygen level during CA storage. The objective of this study was to invest...

  16. Normothermic ex-situ liver preservation: the new gold standard.

    PubMed

    Laing, Richard W; Mergental, Hynek; Mirza, Darius F

    2017-06-01

    Normothermic machine perfusion of the liver (NMP-L) is a novel technology recently introduced into the practice of liver transplantation. This review recapitulates benefits of normothermic perfusion over conventional static cold storage and summarizes recent publications in this area. The first clinical trials have demonstrated both safety and feasibility of NMP-L. They have shown that machine perfusion can entirely replace cold storage or be commenced following a period of cold ischaemia. The technology currently allows transplant teams to extend the period of organ preservation for up to 24 h. Results from the first randomized control trial comparing NMP-L with static cold storage will be available soon. One major advantage of NMP-L technology over other parallel technologies is the potential to assess liver function during NMP-L. Several case series have suggested parameters usable for liver viability testing during NMP-L including bile production and clearance of lactic acidosis. NMP-L allows viability testing of high-risk livers. It has shown the potential to increase utilization of donor organs and improve transplant procedure logistics. NMP-L is likely to become an important technology that will improve organ preservation as well as have the potential to improve utilization of extended criteria donor livers.

  17. 17. NORTHEAST CORNER OF BUILDING 345 (ENTRY CONTROL BUILDING) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. NORTHEAST CORNER OF BUILDING 345 (ENTRY CONTROL BUILDING) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  18. Is freezing in the vaccine cold chain an ongoing issue? A literature review.

    PubMed

    Hanson, Celina M; George, Anupa M; Sawadogo, Adama; Schreiber, Benjamin

    2017-04-19

    Vaccine exposure to temperatures below recommended ranges in the cold chain may decrease vaccine potency of freeze-sensitive vaccines leading to a loss of vaccine investments and potentially places children at risk of contracting vaccine preventable illnesses. This literature review is an update to one previously published in 2007 (Matthias et al., 2007), analyzing the prevalence of vaccine exposure to temperatures below recommendations throughout various segments of the cold chain. Overall, 45 studies included in this review assess temperature monitoring, of which 29 specifically assess 'too cold' temperatures. The storage segments alone were evaluated in 41 articles, 15 articles examined the transport segment and 4 studied outreach sessions. The sample size of the studies varied, ranging from one to 103 shipments and from three to 440 storage units. Among reviewed articles, the percentage of vaccine exposure to temperatures below recommended ranges during storage was 33% in wealthier countries and 37.1% in lower income countries. Vaccine exposure to temperatures below recommended ranges occurred during shipments in 38% of studies from higher income countries and 19.3% in lower income countries. This review highlights continuing issues of vaccine exposure to temperatures below recommended ranges during various segments of the cold chain. Studies monitoring the number of events vaccines are exposed to 'too cold' temperatures as well as the duration of these events are needed. Many reviewed studies emphasize the lack of knowledge of health workers regarding freeze damage of vaccines and how this has an effect on temperature monitoring. It is important to address this issue by educating vaccinators and cold chain staff to improve temperature maintenance and supply chain management, which will facilitate the distribution of potent vaccines to children. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  19. Comment on "Rapid cooling and cold storage in a silicic magma reservoir recorded in individual crystals".

    PubMed

    Wilson, Colin J N; Morgan, Daniel J; Charlier, Bruce L A; Barker, Simon J

    2017-12-22

    Rubin et al (Reports, 16 June 2017, p. 1154) proposed that gradients in lithium abundance in zircons from a rhyolitic eruption in New Zealand reflected short-lived residence at magmatic temperatures interleaved with long-term "cold" (<650°C) storage. Important issues arise with the interpretation of these lithium gradients and consequent crystal thermal histories that raise concerns about the validity of this conclusion. Copyright © 2017, American Association for the Advancement of Science.

  20. Project Themis Supercritical Cold Flow Facility, Experiment Design and Modeling for the Study of Fluid Mixing

    DTIC Science & Technology

    2012-06-01

    AFRL facility was well suited for the Themis cold flow experiment. A test cell was selected that contained an insulated cryogenic oxygen tank that...could be used for the LN2 supply. Adjacent to the test cell is a cryogenic storage bunker that contained a helium supply tank with existing high...venturi to the fuel bunker tank was very low (less than 25 psi) while the helium pressure drop from the cryogenic storage bunker was almost 2000 psi

  1. One or 4 h of "in-house" reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys.

    PubMed

    Gallinat, Anja; Efferz, Patrik; Paul, Andreas; Minor, Thomas

    2014-11-01

    In-house machine perfusion after cold storage (hypothermic reconditioning) has been proposed as convenient tool to improve kidney graft function. This study investigated the role of machine perfusion duration for early reperfusion parameters in porcine kidneys. Kidney function after cold preservation (4 °C, 18 h) and subsequent reconditioning by one or 4 h of pulsatile, nonoxygenated hypothermic machine perfusion (HMP) was studied in an isolated kidney perfusion model in pigs (n = 6, respectively) and compared with simply cold-stored grafts (CS). Compared with CS alone, one or 4 h of subsequent HMP similarly and significantly improved renal flow and kidney function (clearance and sodium reabsorption) upon warm reperfusion, along with reduced perfusate concentrations of endothelin-1 and increased vascular release of nitric oxide. Molecular effects of HMP comprised a significant (vs CS) mRNA increase in the endothelial transcription factor KLF2 and lower expression of endothelin that were observed already at the end of one-hour HMP after CS. Reconditioning of cold-stored kidneys is possible, even if clinical logistics only permit one hour of therapy, while limited extension of the overall storage time by in-house machine perfusion might also allow for postponing of transplantation from night to early day work. © 2014 Steunstichting ESOT.

  2. Thermal environment analysis and energy conservation research of rural residence in cold regions of China based on BIM platform

    NASA Astrophysics Data System (ADS)

    Dong, J. Y.; Cheng, W.; Ma, C. P.; Xin, L. S.; Tan, Y. T.

    2017-06-01

    In order to study the issue of rural residential energy consumption in cold regions of China, modeled an architecture prototype based on BIM platform according to the affecting factors of rural residential thermal environment, and imported the virtual model which contains building information into energy analysis tools and chose the appropriate building orientation. By analyzing the energy consumption of the residential buildings with different enclosure structure forms, we designed the optimal energy-saving residence form. There is a certain application value of this method for researching the energy consumption and energy-saving design for the rural residence in cold regions of China.

  3. Finite element analysis of composite beam-to-column connection with cold-formed steel section

    NASA Astrophysics Data System (ADS)

    Firdaus, Muhammad; Saggaff, Anis; Tahir, Mahmood Md

    2017-11-01

    Cold-formed steel (CFS) sections are well known due to its lightweight and high structural performance which is very popular for building construction. Conventionally, they are used as purlins and side rails in the building envelopes of the industrial buildings. Recent research development on cold-formed steel has shown that the usage is expanded to the use in composite construction. This paper presents the modelling of the proposed composite connection of beam-to-column connection where cold-formed steel of lipped steel section is positioned back-to-back to perform as beam. Reinforcement bars is used to perform the composite action anchoring to the column and part of it is embedded into a slab. The results of the finite element and numerical analysis has showed good agreement. The results show that the proposed composite connection contributes to significant increase to the moment capacity.

  4. 42. CONTEXT VIEW LOOKING WEST OF BUILDING 269 (PAINT BUILDING) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    42. CONTEXT VIEW LOOKING WEST OF BUILDING 269 (PAINT BUILDING) IN ASSEMBLY AREA SHOWING BUILDING 272 (STORAGE STRUCTURE A-2) IN BACKGROUND. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  5. Optimization of Domestic-Size Renewable Energy System Designs Suitable for Cold Climate Regions

    NASA Astrophysics Data System (ADS)

    Akpan, Itoro Etim; Sasaki, Masafumi; Endoh, Noboru

    Five different kinds of domestic-size renewable energy system configurations for very cold climate regions were investigated. From detailed numerical modeling and system simulations, it was found that the consumption of fuel oil for the auxiliary boiler in residential-type households can almost be eliminated with a renewable energy system that incorporates photovoltaic panel arrays for electricity generation and two storage tanks: a well-insulated electric water storage tank that services the hot water loads, and a compact boiler/geothermal heat pump tank for room heating during very cold seasons. A reduction of Greenhouse Gas Emissions (GHG) of about 28% was achieved for this system compared to an equivalent conventional system. The near elimination of the use of fuel oil in this system makes it very promising for very cold climate regions in terms of energy savings because the running cost is not so dependent on the unstable nature of global oil prices.

  6. 16. EAST FRONT ELEVATION OF BUILDING 345 (ENTRY CONTROL BUILDING) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. EAST FRONT ELEVATION OF BUILDING 345 (ENTRY CONTROL BUILDING) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  7. Modelling the effects of Prairie wetlands on streamflow

    NASA Astrophysics Data System (ADS)

    Shook, K.; Pomeroy, J. W.

    2015-12-01

    Recent research has demonstrated that the contributing areas of Prairie streams dominated by depressional (wetland) storage demonstrate hysteresis with respect to catchment water storage. As such contributing fractions can vary over time from a very small percentage of catchment area to the entire catchment during floods. However, catchments display complex memories of past storage states and their contributing fractions cannot be modelled accurately by any single-valued function. The Cold Regions Hydrological Modelling platform, CRHM, which is capable of modelling all of the hydrological processes of cold regions using a hydrological response unit discretization of the catchment, was used to further investigate dynamical contributing area response to hydrological processes. Contributing fraction in CRHM is also controlled by the episodic nature of runoff generation in this cold, sub-humid environment where runoff is dominated by snowmelt over frozen soils, snowdrifts define the contributing fraction in late spring, unfrozen soils have high water holding capacity and baseflow from sub-surface flow does not exist. CRHM was improved by adding a conceptual model of individual Prairie depression fill and spill runoff generation that displays hysteresis in the storage - contributing fraction relationship and memory of storage state. The contributing area estimated by CRHM shows strong sensitivity to hydrological inputs, storage and the threshold runoff rate chosen. The response of the contributing area to inputs from various runoff generating processes from snowmelt to rain-on-snow to rainfall with differing degrees of spatial variation was investigated as was the importance of the memory of storage states on streamflow generation. The importance of selecting hydrologically and ecologically meaningful runoff thresholds in estimating contributing area is emphasized.

  8. PLAN VIEW OF FUEL STORAGE BUILDING (CPP603) SHOWING STORAGE BASINS. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PLAN VIEW OF FUEL STORAGE BUILDING (CPP-603) SHOWING STORAGE BASINS. INL DRAWING NUMBER 200-0603-00-706-051285. ALTERNATE ID NUMBER CPP-D-1285. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  9. Hepatitis B vaccine freezing in the Indonesian cold chain: evidence and solutions.

    PubMed

    Nelson, Carib M; Wibisono, Hariadi; Purwanto, Hary; Mansyur, Isa; Moniaga, Vanda; Widjaya, Anton

    2004-02-01

    To document and characterize freezing temperatures in the Indonesian vaccine cold chain and to evaluate the feasibility of changes designed to reduce the occurrence of freezing. Data loggers were used to measure temperatures of shipments of hepatitis B vaccine from manufacturer to point of use. Baseline conditions and three intervention phases were monitored. During each of the intervention phases, vaccines were removed progressively from the standard 2-8 degrees C cold chain. Freezing temperatures were recorded in 75% of baseline shipments. The highest rates of freezing occurred during transport from province to district, storage in district-level ice-lined refrigerators, and storage in refrigerators in health centres. Interventions reduced freezing, without excessive heat exposure. Inadvertent freezing of freeze-sensitive vaccines is widespread in Indonesia. Simple strategies exist to reduce freezing - for example, selective transport and storage of vaccines at ambient temperatures. The use of vaccine vial monitors reduces the risk associated with heat-damaged vaccines in these scenarios. Policy changes that allow limited storage of freeze-sensitive vaccines at temperatures >2-8 degrees C would enable flexible vaccine distribution strategies that could reduce vaccine freezing, reduce costs, and increase capacity.

  10. Prolonged cold storage of red blood cells by oxygen removal and additive usage

    DOEpatents

    Bitensky, M.W.; Yoshida, Tatsuro

    1998-08-04

    Prolonged cold storage of red blood cells by oxygen removal and additive usage. A cost-effective, 4 C storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. The improved in vivo survival and the preservation of adenosine triphosphate levels, along with reduction in hemolysis and membrane vesicle production of red blood cells stored at 4 C for prolonged periods of time, is achieved by reducing the oxygen level therein at the time of storage; in particular, by flushing the cells with an inert gas, and storing them in an aqueous solution which includes adenine, dextrose, mannitol, citrate ion, and dihydrogen phosphate ion, but no sodium chloride, in an oxygen-permeable container which is located in an oxygen-free environment containing oxygen-scavenging materials. 8 figs.

  11. Prolonged cold storage of red blood cells by oxygen removal and additive usage

    DOEpatents

    Bitensky, Mark W.; Yoshida, Tatsuro

    1998-01-01

    Prolonged cold storage of red blood cells by oxygen removal and additive usage. A cost-effective, 4.degree. C. storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. The improved in vivo survival and the preservation of adenosine triphosphate levels, along with reduction in hemolysis and membrane vesicle production of red blood cells stored at 4.degree. C. for prolonged periods of time, is achieved by reducing the oxygen level therein at the time of storage; in particular, by flushing the cells with an inert gas, and storing them in an aqueous solution which includes adenine, dextrose, mannitol, citrate ion, and dihydrogen phosphate ion, but no sodium chloride, in an oxygen-permeable container which is located in an oxygen-free environment containing oxygen-scavenging materials.

  12. Fructose 1-6 bisphosphate versus University of Wisconsin solution for rat liver preservation: does FBP prevent early mitochondrial injury?

    PubMed

    de Fraga, R S; Heinen, P E T; Kruel, C R P; Molin, S D; Mota, S M; Cerski, C T S; Gasperin, G; Souto, A A; de Oliveira, J R; Alvares-da-Silva, M R

    2011-06-01

    Fructose 1,6-biphosphate (FBP) has been shown to exert therapeutic effects in models of ischemia-reperfusion in organs other than the liver. This study compared FBP and University of Wisconsin (UW) solution during cold storage and reperfusion, among mitochondria of adult male Wistar rat livers. Adult male Wistar rats were assigned to two groups according to the preservation solution used; UW or FBP Aspartate transaminase (AST), alanine transferase (ALT); and lactic dehydrogenase (LDH) were measured in samples of the storage solution obtained at 2, 4 and 6 hours of preservation. After 6 hours of cold storage, we reperfused the liver, taking blood samples to measure AST, ALT, LDH, and throbarbituric acid reactive substances (TBARS). Hepatic fragments were processed for histologic analysis; for determinations of TBARS, catalase, and nitric oxide as well as for mitochondrial evaluation by infrared spectroscopy. During cold preservation, levels of AST and LDH in the storage solution were lower among the FBP group, but after reperfusion, serum levels of AST, ALT, and LDH were higher in this group, as was catalase activity. TBARS and nitric oxide were comparable between the groups. In the UW group there was a higher amide I/amide II ratio than in the FBP group, suggesting an abnormal protein structure of the mitochondrial membrane. No signs of preservation injury were observed in any liver biopsy, but sinusoidal congestion was present in livers preserved with FBP. FBP showed a protective effect for preservation during cold storage seeming to protect the mitochondrial membrane although it did not prevent reperfusion injury. Copyright © 2011 Elsevier Inc. All rights reserved.

  13. [In vitro study of the flow duration of antibiotics solutions prepared in elastomeric infusion devices: effect of cold storage for 3 to 7days].

    PubMed

    Grangeon-Chapon, C; Robein-Dobremez, M-J; Pin, I; Trouiller, P; Allenet, B; Foroni, L

    2015-09-01

    Within the cystic fibrosis patients' home care, EMERAA network ("Together against Cystic fibrosis in Rhone-Alpes and Auvergne") organizes parenteral antibiotics cures at home prepared in elastomeric infusion devices by hospital pharmacies. However, patients and nurses found that the durations of infusion with these devices were often longer than the nominal duration of infusion indicated by their manufacturer. This study aimed to identify the potential different causes in relation to these discordances. Three hundred and ninety devices of two different manufacturers are tested in different experimental conditions: three antibiotics each at two different doses, duration of cold storage (three days or seven days) or immediate tests without cold storage, preparation and storage of the solution in the device (protocol Device) or transfer in the device just before measurement (protocol Pocket). All tests highlighted a longer flow duration for devices prepared according to the protocol Device versus the protocol Pocket (P=0.004). Flow duration is increased in the case of high doses of antibiotics with high viscosity such as piperacilline/tazobactam. The results of this in vitro study showed the impact of: (1) the time between the filling of the device and the flow of the solution; (2) cold storage of elastomeric infusion devices; (3) concentration of antibiotics and therefore the viscosity of the solution to infuse. It is therefore essential that health care teams are aware of factors, which may lead to longer infusion durations with these infusion devices. When the additional time for infusion remain acceptable, it should be necessary to inform the patient and to relativize these lengthening compared to many benefits that these devices provide for home care. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  14. [Managing the cold chain in healthcare facilities].

    PubMed

    Royer, Mathilde; Breton Marchand, Justine; Pons, David

    2017-11-01

    The storage of temperature-sensitive healthcare products requires control of the cold chain. Healthcare facilities must have the appropriate equipment at their disposal and ensure the traceability and monitoring of temperatures. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  15. 1. EXTERIOR OF BUILDING 402, CIVIL ENGINEERING STORAGE, LOOKING SOUTH. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. EXTERIOR OF BUILDING 402, CIVIL ENGINEERING STORAGE, LOOKING SOUTH. - Mill Valley Air Force Station, Civil Engineering Storage, East Ridgecrest Boulevard, Mount Tamalpais, Mill Valley, Marin County, CA

  16. Tolerance of Ruppia sinensis Seeds to Desiccation, Low Temperature, and High Salinity With Special Reference to Long-Term Seed Storage.

    PubMed

    Gu, Ruiting; Zhou, Yi; Song, Xiaoyue; Xu, Shaochun; Zhang, Xiaomei; Lin, Haiying; Xu, Shuai; Yue, Shidong; Zhu, Shuyu

    2018-01-01

    Seeds are important materials for the restoration of globally-threatened marine angiosperm (seagrass) populations. In this study, we investigated the differences between different Ruppia sinensis seed types and developed two feasible long-term R. sinensis seed storage methods. The ability of R. sinensis seeds to tolerate the short-term desiccation and extreme cold had been investigated. The tolerance of R. sinensis seeds to long-term exposure of high salinity, cold temperature, and desiccation had been considered as potential methods for long-term seed storage. Also, three morphological and nine physiological indices were measured and compared between two types of seeds: Shape L and Shape S. We found that: (1) wet storage at a salinity of 30-40 psu and 0°C were the optimal long-term storage conditions, and the proportion of viable seeds reached over 90% after a storage period of 11 months since the seeds were collected from the reproductive shoots; (2) dry condition was not the optimal choice for long-term storage of R. sinensis seeds; however, storing seeds in a dry condition at 5°C and 33 ± 10% relative humidity for 9 months had a relatively high percentage (74.44 ± 2.22%) of viable seeds, consequently desiccation exposure could also be an acceptable seed storage method; (3) R. sinensis seeds would lose vigor in the interaction of extreme cold (-27°C) and desiccation; (4) there were significant differences in seed weight, seed curvature, and endocarp thickness between the two types of seeds. These findings provided fundamental physiological information for R. sinensis seeds and supported the long-term storage of its seeds. Our results may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.

  17. Ob/ob Mouse Livers Show Decreased Oxidative Phosphorylation Efficiencies and Anaerobic Capacities after Cold Ischemia

    PubMed Central

    Tagaloa, Sherry; Zhang, Linda; Dare, Anna J.; MacDonald, Julia R.; Yeong, Mee-Ling; Bartlett, Adam S. J. R.; Phillips, Anthony R. J.

    2014-01-01

    Background Hepatic steatosis is a major risk factor for graft failure in liver transplantation. Hepatic steatosis shows a greater negative influence on graft function following prolonged cold ischaemia. As the impact of steatosis on hepatocyte metabolism during extended cold ischaemia is not well-described, we compared markers of metabolic capacity and mitochondrial function in steatotic and lean livers following clinically relevant durations of cold preservation. Methods Livers from 10-week old leptin-deficient obese (ob/ob, n = 9) and lean C57 mice (n = 9) were preserved in ice-cold University of Wisconsin solution. Liver mitochondrial function was then assessed using high resolution respirometry after 1.5, 3, 5, 8, 12, 16 and 24 hours of storage. Metabolic marker enzymes for anaerobiosis and mitochondrial mass were also measured in conjunction with non-bicarbonate tissue pH buffering capacity. Results Ob/ob and lean mice livers showed severe (>60%) macrovesicular and mild (<30%) microvesicular steatosis on Oil Red O staining, respectively. Ob/ob livers had lower baseline enzymatic complex I activity but similar adenosine triphosphate (ATP) levels compared to lean livers. During cold storage, the respiratory control ratio and complex I-fueled phosphorylation deteriorated approximately twice as fast in ob/ob livers compared to lean livers. Ob/ob livers also demonstrated decreased ATP production capacities at all time-points analyzed compared to lean livers. Ob/ob liver baseline lactate dehydrogenase activities and intrinsic non-bicarbonate buffering capacities were depressed by 60% and 40%, respectively compared to lean livers. Conclusions Steatotic livers have impaired baseline aerobic and anaerobic capacities compared to lean livers, and mitochondrial function indices decrease particularly from after 5 hours of cold preservation. These data provide a mechanistic basis for the clinical recommendation of shorter cold storage durations in steatotic donor livers. PMID:24956382

  18. BUILDING PLANS OF FUEL STORAGE BUILDING (CPP603). INL DRAWING NUMBER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BUILDING PLANS OF FUEL STORAGE BUILDING (CPP-603). INL DRAWING NUMBER 200-0603-61-299-103029. ALTERNATE ID NUMBER 542-31-B-21. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  19. Deep influence of passive low energy consumption multi-storey residential building in cold region

    NASA Astrophysics Data System (ADS)

    Shuai, Zhang; Lihua, Zhao; Rong, Jin; Dong, Junyan

    2018-02-01

    The example of passive architecture demonstration building in Jilin Province, China, based on the practical experience of this project, the control index of passive and low energy consumption residential buildings in cold and passive buildings is referenced by reference to the German construction standard and the Chinese residence construction document, “passive ultra-low energy consumption green Building Technology Guide (Trial)”. The requirement of passive low energy residential buildings on the ground heat transfer coefficient limits is determined, and the performance requirements of passive residential buildings are discussed. This paper analyzes the requirement of the passive low energy residential building on the ground heat transfer coefficient limit, and probes into the influence factors of the ground thermal insulation of the passive low energy consumption residential building. The construction method of passive low energy consumption residential building is proposed.

  20. 5. OBLIQUE INTERIOR VIEW OF CHEMICAL STORAGE BUILDING (#1776), LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. OBLIQUE INTERIOR VIEW OF CHEMICAL STORAGE BUILDING (#1776), LOOKING SOUTHEAST - Presidio Water Treatment Plant, Chemical Storage, East of Lobos Creek at Baker Beach, San Francisco, San Francisco County, CA

  1. 4. DETAIL VIEW OF WINDOW AT CHEMICAL STORAGE BUILDING (#1776), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. DETAIL VIEW OF WINDOW AT CHEMICAL STORAGE BUILDING (#1776), LOOKING EAST - Presidio Water Treatment Plant, Chemical Storage, East of Lobos Creek at Baker Beach, San Francisco, San Francisco County, CA

  2. Canister Storage Building (CSB) Design Basis Accident Analysis Documentation

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    CROWE, R.D.; PIEPHO, M.G.

    2000-03-23

    This document provided the detailed accident analysis to support HNF-3553, Spent Nuclear Fuel Project Final Safety Analysis Report, Annex A, ''Canister Storage Building Final Safety Analysis Report''. All assumptions, parameters, and models used to provide the analysis of the design basis accidents are documented to support the conclusions in the Canister Storage Building Final Safety Analysis Report.

  3. Transcriptomic analysis of (group I) Clostridium botulinum ATCC 3502 cold shock response.

    PubMed

    Dahlsten, Elias; Isokallio, Marita; Somervuo, Panu; Lindström, Miia; Korkeala, Hannu

    2014-01-01

    Profound understanding of the mechanisms foodborne pathogenic bacteria utilize in adaptation to the environmental stress they encounter during food processing and storage is of paramount importance in design of control measures. Chill temperature is a central control measure applied in minimally processed foods; however, data on the mechanisms the foodborne pathogen Clostridium botulinum activates upon cold stress are scarce. Transcriptomic analysis on the C. botulinum ATCC 3502 strain upon temperature downshift from 37°C to 15°C was performed to identify the cold-responsive gene set of this organism. Significant up- or down-regulation of 16 and 11 genes, respectively, was observed 1 h after the cold shock. At 5 h after the temperature downshift, 199 and 210 genes were up- or down-regulated, respectively. Thus, the relatively small gene set affected initially indicated a targeted acute response to cold shock, whereas extensive metabolic remodeling appeared to take place after prolonged exposure to cold. Genes related to fatty acid biosynthesis, oxidative stress response, and iron uptake and storage were induced, in addition to mechanisms previously characterized as cold-tolerance related in bacteria. Furthermore, several uncharacterized DNA-binding transcriptional regulator-encoding genes were induced, suggesting involvement of novel regulatory mechanisms in the cold shock response of C. botulinum. The role of such regulators, CBO0477 and CBO0558A, in cold tolerance of C. botulinum ATCC 3502 was demonstrated by deteriorated growth of related mutants at 17°C.

  4. Sandia National Laboratories, Tonopah Test Range Assembly Building 9B (Building 09-54): Photographs and Written Historical and Descriptive Data

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ullrich, Rebecca A.

    Assembly Building 9B (Building 09-54) is a contributing element to the Sandia National Laboratories (SNL) Tonopah Test Range (TTR) Historic District. The SNL TTR Historic District played a significant role in U.S. Cold War history in the areas of stockpile surveillance and non-nuclear field testing of nuclear weapons designs. The district covers approximately 179,200 acres and illustrates Cold War development testing of nuclear weapons components and systems. This report includes historical information, architectural information, sources of information, project information, maps, blueprints, and photographs.

  5. Hen genetic strain and extended cold storage influence on physical egg quality from cage-free aviary housing system.

    PubMed

    Jones, D R; Karcher, D M; Regmi, P; Robison, C O; Gast, R K

    2018-04-02

    In the United States, there is an increase in need for cage-free eggs in retail and food manufacturing sectors. Understanding the impact of cage-free systems and the corresponding management on egg quality is pertinent as the U.S. industry adapts existing housing and builds new cage-free housing structures. A study was conducted comparing 2 brown shell and 2 white shell hen strains housed in a cage-free aviary system. Each set of eggs were placed in cold storage and assessed at 0, 2, 4, 8, and 12 wk. Eggs were collected at 21, 31, 42, and 60 wk of hen age. A full profile of physical quality measurements was conducted on up to 18 intact eggs for each hen strain/egg storage/hen age combination. Egg weight increased approximately 10 g for brown shell and 14 g for white shell eggs as hens aged. Many of the properties monitored were significantly impacted by all 3 main effects (hen strain, egg storage, and hen age) resulting in 3-way interactions. A brown and a white shell strain had stronger shells (44 N; P < 0.0001) than the remaining brown and white shell strains (42 N and 39 N, respectively). The current study also determined volume of shell, total length, maximum width, and percent length at maximum width to more accurately indicate egg shape than shape index. One brown shell strain produced eggs with the most consistent shape characteristics over the hen ages monitored. White shell eggs from the cage-free aviary housing produced the highest whole-egg total solids between 31 to 60 wk of hen age, whereas brown shell eggs resulted in the most consistent level of whole-egg total solids (22-23.5%). The brown and white shell strains in the current study produce cage-free aviary eggs with distinctive physical quality attributes. The outcomes from this study can be utilized by the U.S. egg industry in planning management strategies and market placement of cage-free eggs.

  6. Liquid Hydrogen Fill

    NASA Image and Video Library

    2016-08-03

    Inside a control building at NASA's Kennedy Space Center in Florida, Adam Swinger, cryogenic research engineer in the Exploration Research and Technology Directorate, communicates with team members during a test of the Ground Operations Demo Unit for liquid hydrogen. The system includes a 33,000 gallon liquid hydrogen storage tank with an internal cold heat exchanger supplied from a cryogenic refrigerator. The primary goal of the testing is to achieve a liquid hydrogen zero boil-off capability. The system was designed, installed and tested by a team of civil servants and contractors from the center's Cryogenic Test Laboratory, with support from engineers at NASA's Glenn Research Center in Cleveland and Stennis Space Center in Mississippi. It may be applicable for use by the Ground Systems Development and Operations Program at Launch Pad 39B.

  7. 21. SITE BUILDING 002 SCANNER BUILDING LOOKING AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. SITE BUILDING 002 - SCANNER BUILDING - LOOKING AT DISC STORAGE SYSTEMS A AND B (A OR B ARE REDUNDANT SYSTEMS), ONE MAINFRAME COMPUTER ON LINE, ONE ON STANDBY WITH STORAGE TAPE, ONE ON STANDBY WITHOUT TAPE INSTALLED. - Cape Cod Air Station, Technical Facility-Scanner Building & Power Plant, Massachusetts Military Reservation, Sandwich, Barnstable County, MA

  8. High throughput sequencing identifies chilling responsive genes in sweetpotato (Ipomoea batatas Lam.) during storage.

    PubMed

    Xie, Zeyi; Zhou, Zhilin; Li, Hongmin; Yu, Jingjing; Jiang, Jiaojiao; Tang, Zhonghou; Ma, Daifu; Zhang, Baohong; Han, Yonghua; Li, Zongyun

    2018-05-21

    Sweetpotato (Ipomoea batatas L.) is a globally important economic food crop. It belongs to Convolvulaceae family and origins in the tropics; however, sweetpotato is sensitive to cold stress during storage. In this study, we performed transcriptome sequencing to investigate the sweetpotato response to chilling stress during storage. A total of 110,110 unigenes were generated via high-throughput sequencing. Differentially expressed genes (DEGs) analysis showed that 18,681 genes were up-regulated and 21,983 genes were down-regulated in low temperature condition. Many DEGs were related to the cell membrane system, antioxidant enzymes, carbohydrate metabolism, and hormone metabolism, which are potentially associated with sweetpotato resistance to low temperature. The existence of DEGs suggests a molecular basis for the biochemical and physiological consequences of sweetpotato in low temperature storage conditions. Our analysis will provide a new target for enhancement of sweetpotato cold stress tolerance in postharvest storage through genetic manipulation. Copyright © 2018. Published by Elsevier Inc.

  9. An effect of surface properties on detachment of adhered solid to cooling surface for formation of clathrate hydrate slurry

    NASA Astrophysics Data System (ADS)

    Daitoku, Tadafumi; Utaka, Yoshio

    In air-conditioning systems, it is desirable that the liquid-solid phase change temperature of a cool energy storage material is approximately 10 °C from the perspective of improving coefficient of performance (COP). Moreover, a thermal storage material that forms slurry can realize large heat capacity of working fluids. Since the solid that adheres to the heat transfer surface forms a thermal resistance layer and remarkably reduces the rate of cold storage, it is important to avoid the adhesion of a thick solid layer on the surface so as to realize efficient energy storage. Considering a harvest type cooling unit, the force required for removing the solid phase from the heat transfer surface was studied. Tetra-n-butylammonium Bromide (TBAB) clathrate hydrate was used as a cold storage material. The effect of the heat transfer surface properties on the scraping force for detachment of adhered solid of TBAB hydrate to the heat transfer surface was examined experimentally.

  10. The effect of fasting and body reserves on cold tolerance in 2 pit-building insect predators.

    PubMed

    Scharf, Inon; Daniel, Alma; MacMillan, Heath Andrew; Katz, Noa

    2017-06-01

    Pit-building antlions and wormlions are 2 distantly-related insect species, whose larvae construct pits in loose soil to trap small arthropod prey. This convergent evolution of natural histories has led to additional similarities in their natural history and ecology, and thus, these 2 species encounter similar abiotic stress (such as periodic starvation) in their natural habitat. Here, we measured the cold tolerance of the 2 species and examined whether recent feeding or food deprivation, as well as body composition (body mass and lipid content) and condition (quantified as mass-to-size residuals) affect their cold tolerance. In contrast to other insects, in which food deprivation either enhanced or impaired cold tolerance, prolonged fasting had no effect on the cold tolerance of either species, which had similar cold tolerance. The 2 species differed, however, in how cold tolerance related to body mass and lipid content: although body mass was positively correlated with the wormlion cold tolerance, lipid content was a more reliable predictor of cold tolerance in the antlions. Cold tolerance also underwent greater change with ontogeny in wormlions than in antlions. We discuss possible reasons for this lack of effect of food deprivation on both species' cold tolerance, such as their high starvation tolerance (being sit-and-wait predators).

  11. PLOT PLAN OF FUEL STORAGE BUILDING (CPP603) SHOWING STORAGE BASINS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PLOT PLAN OF FUEL STORAGE BUILDING (CPP-603) SHOWING STORAGE BASINS AND PROPOSED LOCATION OF FUEL ELEMENT CUTTING FACILITY. INL DRAWING NUMBER 200-0603-00-706-051287. ALTERNATE ID NUMBER CPP-C-1287. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  12. 3. OBLIQUE DETAIL VIEW OF DOOR AT CHEMICAL STORAGE BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. OBLIQUE DETAIL VIEW OF DOOR AT CHEMICAL STORAGE BUILDING (#1776), LOOKING NORTHWEST - Presidio Water Treatment Plant, Chemical Storage, East of Lobos Creek at Baker Beach, San Francisco, San Francisco County, CA

  13. 62. SOUTH CORNER OF BUILDING 372 (HAZARDOUS STORAGE) IN BASE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    62. SOUTH CORNER OF BUILDING 372 (HAZARDOUS STORAGE) IN BASE SPARES AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  14. Genetic and biochemical bases of superficial scald storage disorder in apple and pear fruits

    USDA-ARS?s Scientific Manuscript database

    Superficial scald is a physiological storage disorder affecting apple and pear fruits. The disorder develops during cold storage and intensifies after removal to market temperatures. Scald symptoms result from necrosis of a few hypodermal cell layers and manifest as brown or black patches on the fru...

  15. Influence of critical storage temperatures on degradative pathways of pigments in green beans (Phaseolus vulgaris cvs. Perona and boby).

    PubMed

    Monreal, M; De Ancos, B; Cano, M P

    1999-01-01

    In this work a study of critical storage temperatures on pigment degradation of green beans (Phaseolus vulgaris, cvs. Perona and Boby) was conducted. In this way, green beans kept better quality at 4 degrees C than either 8 or 12 degrees C, maintaining a bright green color and good texture. Nevertheless, temperatures of 4 degrees C induced chilling injury (CI) after eight days of storage, which became evident when the pods were transferred to 20 degrees C. Cold storage temperatures, 12, 8, and 4 degrees C, produced different changes on the green beans chlorophyll profile. Green beans of both cultivars, Perona and Boby, stored at 4 and 12 degrees C showed a continuous degradation of chlorophyll pigments during storage, while samples stored at 8 degrees C showed an increase of chlorophyll content at the first 15 days. Carotenoid pigments also suffered different changes during cold storage. Perona was the green beans cultivar which maintained the higher level of lutein, mainly when samples were stored at the most suitable temperature (8 degrees C).

  16. Building G interior, second floor oblique looking southwest, showing storage ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Building G interior, second floor oblique looking southwest, showing storage area for samples - Daniel F. Waters Germantown Dye Works, Building G, 37-55 East Wister Street, Philadelphia, Philadelphia County, PA

  17. Cold preservation with hyperbranched polyglycerol-based solution improves kidney functional recovery with less injury at reperfusion in rats

    PubMed Central

    Li, Shadan; Liu, Bin; Guan, Qiunong; Chafeeva, Irina; Brooks, Donald E; Nguan, Christopher YC; Kizhakkedathu, Jayachandran N; Du, Caigan

    2017-01-01

    Minimizing donor organ injury during cold preservation (including cold perfusion and storage) is the first step to prevent transplant failure. We recently reported the advantages of hyperbranched polyglycerol (HPG) as a novel substitute for hydroxyethyl starch in UW solution for both cold heart preservation and cold kidney perfusion. This study evaluated the functional recovery of the kidney at reperfusion after cold preservation with HPG solution. The impact of HPG solution compared to conventional UW and HTK solutions on tissue weight and cell survival at 4°C was examined using rat kidney tissues and cultured human umbilical vein endothelial cells (HUVECs), respectively. The kidney protection by HPG solution was tested in a rat model of cold kidney ischemia-reperfusion injury, and was evaluated by histology and kidney function. Here, we showed that preservation with HPG solution prevented cell death in cultured HUVECs and edema formation in kidney tissues at 4°C similar to UW solution, whereas HTK solution was less effective. In rat model of cold ischemia-reperfusion injury, the kidneys perfused and subsequently stored 1-hour with cold HPG solution showed less leukocyte infiltration, less tubular damage and better kidney function (lower levels of serum creatinine and blood urea nitrogen) at 48 h of reperfusion than those treated with UW or HTK solution. In conclusion, our data show the superiority of HPG solution to UW or HTK solution in the cold perfusion and storage of rat kidneys, suggesting that the HPG solution may be a promising candidate for improved donor kidney preservation prior to transplantation. PMID:28337272

  18. Stabilizing the cold plasma-stimulated medium by regulating medium’s composition

    NASA Astrophysics Data System (ADS)

    Yan, Dayun; Nourmohammadi, Niki; Bian, Ka; Murad, Ferid; Sherman, Jonathan H.; Keidar, Michael

    2016-05-01

    Over past several years, the cold plasma-stimulated medium (PSM) has shown its remarkable anti-cancer capacity in par with the direct cold plasma irradiation on cancer cells or tumor tissues. Independent of the cold plasma device, PSM has noticeable advantage of being a flexible platform in cancer treatment. Currently, the largest disadvantage of PSM is its degradation during the storage over a wide temperature range. So far, to stabilize PSM, it must be remained frozen at -80 °C. In this study, we first reveal that the degradation of PSM is mainly due to the reaction between the reactive species and specific amino acids; mainly cysteine and methionine in medium. Based on this finding, both H2O2 in PSM and the anti-cancer capacity of PSM can be significantly stabilized during the storage at 8 °C and -25 °C for at least 3 days by using phosphate-buffered saline (PBS) and cysteine/methionine-free Dulbecco’s Modified Eagle Medium (DMEM). In addition, we demonstrate that adding a tyrosine derivative, 3-Nitro-L-tyrosine, into DMEM can mitigate the degradation of PSM at 8 °C during 3 days of storage. This study provides a solid foundation for the future anti-cancer application of PSM.

  19. [Maintenance of cold chain for the delivery of immunobiological drugs by the indicators of heating and freezing controls].

    PubMed

    Smirnov, D V; Buianov, V V; Kolesnikov, N V; Minaev, V A; Demina, A M; Suprun, I P

    2004-01-01

    The paper deals with an objective evaluation of a qualitative storage of immunobiological drugs including all stages of "cold chain". The results of technological research and of designing related with constructing a system of indicators for monitoring the temperature regime to maintain the "cold chain" functioning are presented. The suggested devices are comparatively described.

  20. 61. SOUTHEAST FRONT ELEVATION OF BUILDING 372 (HAZARDOUS STORAGE) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    61. SOUTHEAST FRONT ELEVATION OF BUILDING 372 (HAZARDOUS STORAGE) IN BASE SPARES AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  1. Contemporary formulation and distribution practices for cold-filled acid products: Australian industry survey and modeling of published pathogen inactivation data.

    PubMed

    Chapman, B; Scurrah, K J; Ross, T

    2010-05-01

    A survey of 12 Australian manufacturers indicated that mild-tasting acids and preservatives are used to partially replace acetic acid in cold-filled acid dressings and sauces. In contrast to traditional ambient temperature distribution practices, some manufacturers indicated that they supply the food service sector with cold-filled acid products prechilled for incorporation into ready-to-eat foods. The Comité des Industries des Mayonnaises et Sauces Condimentaires de la Communauté Economique Européenne (CIMSCEE) Code, a formulation guideline used by the industry to predict the safety of cold-filled acid formulations with respect to Salmonella enterica and Escherichia coli, does not extend to the use of acids and preservatives other than acetic acid nor does it consider the effects of chill distribution. We found insufficient data in the published literature to comprehensively model the response of S. enterica and E. coli to all of the predictor variables (i.e., pH, acetic acid, NaCl, sugars, other acids, preservatives, and storage temperature) of relevance for contemporary cold-filled acid products in Australia. In particular, we noted a lack of inactivation data for S. enterica at aqueous-phase NaCl concentrations of >3% (wt/wt). However, our simple models clearly identified pH and 1/absolute temperature of storage as the most important variables generally determining inactivation. To develop robust models to predict the effect of contemporary formulation and storage variables on product safety, additional empirical data are required. Until such models are available, our results support challenge testing of cold-filled acid products to ascertain their safety, as suggested by the CIMSCEE, but suggest consideration of challenging with both E. coli and S. enterica at incubation temperatures relevant to intended product distribution temperatures.

  2. The added economic and environmental value of plug-in electric vehicles connected to commercial building microgrids

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stadler, Michael; Momber, Ilan; Megel, Olivier

    2010-08-25

    Connection of electric storage technologies to smartgrids or microgrids will have substantial implications for building energy systems. In addition to potentially supplying ancillary services directly to the traditional centralized grid (or macrogrid), local storage will enable demand response. As an economically attractive option, mobile storage devices such as plug-in electric vehicles (EVs) are in direct competition with conventional stationary sources and storage at the building. In general, it is assumed that they can improve the financial as well as environmental attractiveness of renewable and fossil based on-site generation (e.g. PV, fuel cells, or microturbines operating with or without combined heatmore » and power). Also, mobile storage can directly contribute to tariff driven demand response in commercial buildings. In order to examine the impact of mobile storage on building energy costs and carbon dioxide (CO2) emissions, a microgrid/distributed-energy-resources (DER) adoption problem is formulated as a mixed-integer linear program with minimization of annual building energy costs applying CO2 taxes/CO2 pricing schemes. The problem is solved for a representative office building in the San Francisco Bay Area in 2020. By using employees' EVs for energy management, the office building can arbitrage its costs. But since the car battery lifetime is reduced, a business model that also reimburses car owners for the degradation will be required. In general, the link between a microgrid and an electric vehicle can create a win-win situation, wherein the microgrid can reduce utility costs by load shifting while the electric vehicle owner receives revenue that partially offsets his/her expensive mobile storage investment. For the California office building with EVs connected under a business model that distributes benefits, it is found that the economic impact is very limited relative to the costs of mobile storage for the site analyzed, i.e. cost reductions from electric vehicle connections are modest. Nonetheless, this example shows that some economic benefit is created because of avoided demand charges and on-peak energy. The strategy adopted by the office building is to avoid these high on-peak costs by using energy from the mobile storage in the business hours. CO2 emission reduction strategy results indicate that EVs' contribution at the selected office building are minor.« less

  3. NREL's Energy Storage and REopt Teams Awarded $525k from TCF to Study

    Science.gov Websites

    Commercial Viability of Optimal, Reliable Building-Integrated Energy Storage | News | NREL NREL's Energy Storage and REopt Teams Awarded $525k from TCF to Study Commercial Viability of Optimal Study Commercial Viability of Optimal, Reliable Building-Integrated Energy Storage November 14, 2017

  4. Effect of Thyme and Rosemary on The Quality Characteristics, Shelf-life, and Residual Nitrite Content of Sausages During Cold Storage.

    PubMed

    Jin, Sang Keun; Choi, Jung Seok; Lee, Seung Jae; Lee, Seung Yun; Hur, Sun Jin

    2016-10-31

    The effects of thyme and rosemary on the quality characteristics of sausages during cold storage were investigated. Sausages were prepared with thyme and rosemary powder (1 and 2%) and stored for 6 weeks at 10℃. The pH was significantly decreased in sausages by addition of thyme and rosemary compared to that observed in the control before and after storage. At 4 weeks of storage, the residual nitrite content was decreased by thyme and rosemary compared to the control. Lightness (L*) and yellowness (b*) were increased during storage, whereas redness (a*) and whiteness (W) were decreased before and after storage by addition of thyme and rosemary. The amount of TPC and lactic acid bacteria was lower at the end of storage in sausage containing thyme and rosemary. The 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging capacity of sausages was increased by addition of thyme and rosemary compared to that in the control before and after storage. In particular, T2 (0.2% thyme addition) showed the highest DPPH radical scavenging capacity during storage. In a sensory evaluation, flavor and overall acceptability were lower in sausages containing thyme and rosemary than in the control. However, at the end of storage (6 wk), aroma, flavor and overall acceptability were not significantly different among the sausage samples.

  5. Effect of Thyme and Rosemary on The Quality Characteristics, Shelf-life, and Residual Nitrite Content of Sausages During Cold Storage

    PubMed Central

    Jin, Sang Keun; Choi, Jung Seok; Lee, Seung Jae

    2016-01-01

    The effects of thyme and rosemary on the quality characteristics of sausages during cold storage were investigated. Sausages were prepared with thyme and rosemary powder (1 and 2%) and stored for 6 weeks at 10℃. The pH was significantly decreased in sausages by addition of thyme and rosemary compared to that observed in the control before and after storage. At 4 weeks of storage, the residual nitrite content was decreased by thyme and rosemary compared to the control. Lightness (L*) and yellowness (b*) were increased during storage, whereas redness (a*) and whiteness (W) were decreased before and after storage by addition of thyme and rosemary. The amount of TPC and lactic acid bacteria was lower at the end of storage in sausage containing thyme and rosemary. The 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging capacity of sausages was increased by addition of thyme and rosemary compared to that in the control before and after storage. In particular, T2 (0.2% thyme addition) showed the highest DPPH radical scavenging capacity during storage. In a sensory evaluation, flavor and overall acceptability were lower in sausages containing thyme and rosemary than in the control. However, at the end of storage (6 wk), aroma, flavor and overall acceptability were not significantly different among the sausage samples. PMID:27857542

  6. Oblique view of rear and south sides of ammunition storage ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of rear and south sides of ammunition storage buildings 4403 and 4404, view towards the north without scale - Fort McClellan Ammunition Storage Area, Building No. 4403, Second Avenue (Magazine Road), Anniston, Calhoun County, AL

  7. Oblique view of rear and south sides of ammunition storage ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of rear and south sides of ammunition storage buildings 4404 and 4405, view towards the north with scale - Fort McClellan Ammunition Storage Area, Building No. 4404, Second Avenue (Magazine Road), Anniston, Calhoun County, AL

  8. Oblique view of rear and south sides of ammunition storage ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of rear and south sides of ammunition storage buildings 4404 and 4405, view towards the north without scale - Fort McClellan Ammunition Storage Area, Building No. 4404, Second Avenue (Magazine Road), Anniston, Calhoun County, AL

  9. 45. CONTEXT VIEW LOOKING SOUTH OF BUILDING 272 (STORAGE STRUCTURE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    45. CONTEXT VIEW LOOKING SOUTH OF BUILDING 272 (STORAGE STRUCTURE A-2 IN ASSEMBLY AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  10. 43. EAST FRONT ELEVATION OF BUILDING 272 (STORAGE STRUCTURE A2) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    43. EAST FRONT ELEVATION OF BUILDING 272 (STORAGE STRUCTURE A-2) IN ASSEMBLY AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  11. 6. DETAIL OF ENTRY ON NORTH ELEVATION OF BUILDING 260 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. DETAIL OF ENTRY ON NORTH ELEVATION OF BUILDING 260 (STORAGE STRUCTURE) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  12. Review on cold-formed steel connections.

    PubMed

    Lee, Yeong Huei; Tan, Cher Siang; Mohammad, Shahrin; Tahir, Mahmood Md; Shek, Poi Ngian

    2014-01-01

    The concept of cold-formed light steel framing construction has been widespread after understanding its structural characteristics with massive research works over the years. Connection serves as one of the important elements for light steel framing in order to achieve its structural stability. Compared to hot-rolled steel sections, cold-formed steel connections perform dissimilarity due to the thin-walled behaviour. This paper aims to review current researches on cold-formed steel connections, particularly for screw connections, storage rack connections, welded connections, and bolted connections. The performance of these connections in the design of cold-formed steel structures is discussed.

  13. Review on Cold-Formed Steel Connections

    PubMed Central

    Tan, Cher Siang; Mohammad, Shahrin; Md Tahir, Mahmood; Shek, Poi Ngian

    2014-01-01

    The concept of cold-formed light steel framing construction has been widespread after understanding its structural characteristics with massive research works over the years. Connection serves as one of the important elements for light steel framing in order to achieve its structural stability. Compared to hot-rolled steel sections, cold-formed steel connections perform dissimilarity due to the thin-walled behaviour. This paper aims to review current researches on cold-formed steel connections, particularly for screw connections, storage rack connections, welded connections, and bolted connections. The performance of these connections in the design of cold-formed steel structures is discussed. PMID:24688448

  14. Improved biochemical preservation of lung slices during cold storage.

    PubMed

    Bull, D A; Connors, R C; Reid, B B; Albanil, A; Stringham, J C; Karwande, S V

    2000-05-15

    Development of lung preservation solutions typically requires whole-organ models which are animal and labor intensive. These models rely on physiologic rather than biochemical endpoints, making accurate comparison of the relative efficacy of individual solution components difficult. We hypothesized that lung slices could be used to assess preservation of biochemical function during cold storage. Whole rat lungs were precision cut into slices with a thickness of 500 microm and preserved at 4 degrees C in the following solutions: University of Wisconsin (UW), Euro-Collins (EC), low-potassium-dextran (LPD), Kyoto (K), normal saline (NS), or a novel lung preservation solution (NPS) developed using this model. Lung biochemical function was assessed by ATP content (etamol ATP/mg wet wt) and capacity for protein synthesis (cpm/mg protein) immediately following slicing (0 h) and at 6, 12, 18, and 24 h of cold storage. Six slices were assayed at each time point for each solution. The data were analyzed using analysis of variance and are presented as means +/- SD. ATP content was significantly higher in the lung slices stored in NPS compared with all other solutions at each time point (P < 0.0001). Protein synthesis was significantly higher in the lung slices stored in NPS compared with all other solutions at 6, 12, and 18 h of preservation (P < 0.05). This lung slice model allows the rapid and efficient screening of lung preservation solutions and their components using quantifiable biochemical endpoints. Using this model, we have developed a novel solution that improves the biochemical preservation of lung slices during cold storage. Copyright 2000 Academic Press.

  15. NMR fingerprinting as a tool to evaluate post-harvest time-related changes of peaches, tomatoes and plums.

    PubMed

    Santucci, Claudio; Tenori, Leonardo; Luchinat, Claudio

    2015-09-01

    The time-related changes of three agricultural products, coming from two distribution routes, have been followed using NMR fingerprinting to monitor metabolic variations occurring during several days of cold storage. An NMR profiling approach was employed to evaluate the variations in metabolic profile and metabolite content in three different agricultural products highly consumed in Italy (peaches, tomatoes and plums) coming from Tuscanian farms and how they change with time after collection. For each product, we followed the time-related changes during cold storage along three different collection periods. We monitored the variations in metabolic fingerprint and the trend of a set of metabolites, focusing our attention on nutritive and health-promoting metabolites (mainly, essential amino acids and antioxidants) as well as metabolites that contribute to the taste. Concurrently, for comparison, the time-dependent changes of the same kind of products coming from large-scale distribution have been also analyzed under the same conditions. In this second category, only slight variations in the metabolic fingerprint and metabolite levels were seen during cold storage. Unsupervised and supervised multivariate statistics was also employed to enlighten the differences between the three collections. In particular it seems that the metabolic fingerprint of large-scale distribution products is quite similar in the early, middle and late collection, while peaches and plums locally collected are markedly different among the three periods. The metabolic profiles of the agricultural products belonging to these two different distribution routes are intrinsically different, and they show different changes during the time of cold storage. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. Impact of exogenous GABA treatments on endogenous GABA metabolism in anthurium cut flowers in response to postharvest chilling temperature.

    PubMed

    Aghdam, Morteza Soleimani; Naderi, Roohangiz; Jannatizadeh, Abbasali; Babalar, Mesbah; Sarcheshmeh, Mohammad Ali Askari; Faradonbe, Mojtaba Zamani

    2016-09-01

    Anthurium flowers are susceptible to chilling injury, and the optimum storage temperature is 12.5-20 °C. The γ-aminobutyric acid (GABA) shunt pathway may alleviate chilling stress in horticultural commodities by providing energy (ATP), reducing molecules (NADH), and minimizing accumulation of reactive oxygen species (ROS). In this experiment, the impact of a preharvest spray treatment with 1 mM GABA and postharvest treatment of 5 mM GABA stem-end dipping on GABA shunt pathway activity of anthurium cut flowers (cv. Sirion) in response to cold storage (4 °C for 21 days) was investigated. GABA treatments resulted in lower glutamate decarboxylase (GAD) and higher GABA transaminase (GABA-T) activities in flowers during cold storage, which was associated with lower GABA content and coincided with higher ATP content. GABA treatments also enhanced accumulation of endogenous glycine betaine (GB) in flowers during cold storage, as well as higher spathe relative water content (RWC). These findings suggest that GABA treatments may alleviate chilling injury of anthurium cut flowers by enhancing GABA shunt pathway activity leading to provide sufficient ATP and promoting endogenous GB accumulation. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  17. Study of Cycling Air-Cooling System with a Cold Accumulator for Micro Gas-Turbine Installations

    NASA Astrophysics Data System (ADS)

    Ochkov, V. F.; Stepanova, T. A.; Katenev, G. M.; Tumanovskii, V. A.; Borisova, P. N.

    2018-05-01

    Using the cycling air-cooling systems of the CTIC type (Combustion Turbine Inlet Cooling) with a cold accumulator in a micro gas-turbine installation (micro-GTI) to preserve its capacity under the seasonal temperature rise of outside air is described. Water ice is used as the body-storage in the accumulators, and ice water (water at 0.5-1.0°C) is used as the body that cools air. The ice water circulates between the accumulator and the air-water heat exchanger. The cold accumulator model with renewable ice resources is considered. The model contains the heat-exchanging tube lattice-evaporator covered with ice. The lattice is cross-flowed with water. The criterion heat exchange equation that describes the process in the cold accumulator under consideration is presented. The calculations of duration of its active operation were performed. The dependence of cold accumulator service life on water circulation rate was evaluated. The adequacy of the design model was confirmed experimentally in the mock-up of the cold accumulator with a refrigerating machine periodically creating a 200 kg ice reserve in the reservoir-storage. The design model makes it possible to determine the weight of ice reserve of the discharged cold accumulator for cooling the cycle air in the operation of a C-30 type micro- GTI produced by the Capstone Company or micro-GTIs of other capacities. Recommendations for increasing the working capacity of cold accumulators of CTIC-systems of a micro-GTI were made.

  18. Porphyrin-Based Symmetric Redox-Flow Batteries towards Cold-Climate Energy Storage.

    PubMed

    Ma, Ting; Pan, Zeng; Miao, Licheng; Chen, Chengcheng; Han, Mo; Shang, Zhenfeng; Chen, Jun

    2018-03-12

    Electrochemical energy storage with redox-flow batteries (RFBs) under subzero temperature is of great significance for the use of renewable energy in cold regions. However, RFBs are generally used above 10 °C. Herein we present non-aqueous organic RFBs based on 5,10,15,20-tetraphenylporphyrin (H 2 TPP) as a bipolar redox-active material (anode: [H 2 TPP] 2- /H 2 TPP, cathode: H 2 TPP/[H 2 TPP] 2+ ) and a Y-zeolite-poly(vinylidene fluoride) (Y-PVDF) ion-selective membrane with high ionic conductivity as a separator. The constructed RFBs exhibit a high volumetric capacity of 8.72 Ah L -1 with a high voltage of 2.83 V and excellent cycling stability (capacity retention exceeding 99.98 % per cycle) in the temperature range between 20 and -40 °C. Our study highlights principles for the design of RFBs that operate at low temperatures, thus offering a promising approach to electrochemical energy storage under cold-climate conditions. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. [A cold/heat property classification strategy based on bio-effects of herbal medicines].

    PubMed

    Jiang, Miao; Lv, Ai-Ping

    2014-06-01

    The property theory of Chinese herbal medicine (CHM) is regarded as the core and basic of Chinese medical theory, however, the underlying mechanism of the properties in CHMs remains unclear, which impedes a barrier for the modernization of Chinese herbal medicine. The properties of CHM are often categorized into cold and heat according to the theory of Chinese medicine, which are essential to guide the clinical application of CHMs. There is an urgent demand to build a cold/heat property classification model to facilitate the property theory of Chinese herbal medicine, as well as to clarify the controversial properties of some herbs. Based on previous studies on the cold/heat properties of CHM, in this paper, we described a novel strategy on building a cold/heat property classification model based on herbal bio-effect. The interdisciplinary cooperation of systems biology, pharmacological network, and pattern recognition technique might lighten the study on cold/heat property theory, provide a scientific model for determination the cold/heat property of herbal medicines, and a new strategy for expanding the Chinese herbal medicine resources as well.

  20. Heat stress affects carbohydrate metabolism during cold-induced sweetening of potato (Solanum tuberosum L.).

    PubMed

    Herman, Derek J; Knowles, Lisa O; Knowles, N Richard

    2017-03-01

    Tolerance to heat stress for retention of low-temperature sweetening-resistant phenotype in potato is conferred by insensitivity of acid invertase activity to cold induction. Heat stress exacerbated cold sweetening (buildup of reducing sugars) of the LTS (low-temperature sweetening)-susceptible potato (Solanum tuberosum L.) cultivars, Ranger Russet and Russet Burbank, and completely abolished the resistance to cold sweetening in the LTS-resistant cultivars/clones, Sage Russet, GemStar Russet, POR06V12-3 and A02138-2. Payette Russet and EGA09702-2, however, demonstrated considerable tolerance to heat stress for retention of their LTS-resistant phenotype. Heat-primed Payette Russet and EGA09702-2 tubers accumulated fourfold more sucrose when subsequently stored at 4 °C, while reducing sugar concentrations also increased marginally but remained low relative to the non-heat-tolerant LTS-resistant clones, resulting in light-colored fries. By contrast, sucrose concentrations in heat-primed tubers of the non-heat-tolerant clones remained unchanged during LTS, but reducing sugars increased fivefold, resulting in darkening of processed fries. Acid invertase activity increased in the LTS-susceptible and non-heat-tolerant LTS-resistant cultivars/clones during cold storage. However, Payette Russet tubers maintained very low invertase activity regardless of heat stress and cold storage treatments, as was the case for Innate ® Russet Burbank (W8) tubers, where silenced invertase conferred robust tolerance to heat stress for retention of LTS-resistant phenotype. Importantly, heat-stressed tubers of Payette Russet, EGA09702-2 and Innate ® Russet Burbank (W8) demonstrated similar low reducing sugar and high sucrose-accumulating phenotypes when stored at 4 °C. Tolerance to heat stress for retention of LTS-resistant phenotype in Payette Russet and likely its maternal parent, EGA09702-2, is, therefore, conferred by the ability to maintain low invertase activity during cold storage of heat-stressed tubers.

  1. Determination of Spoilage Microbiota of Pacific White Shrimp During Ambient and Cold Storage Using Next-Generation Sequencing and Culture-Dependent Method.

    PubMed

    Yang, Sheng-Ping; Xie, Jing; Qian, Yun-Fang

    2017-05-01

    This study was conducted to determine the initial and spoilage microbiota of Pacific white shrimp during ambient and cold storage using next-generation sequencing (NGS) and a culture-dependent method. The quality changes were also evaluated by sensory analysis and total volatile basic nitrogen (TVB-N) values. After 1 d of storage, the psychrotrophic bacteria were only 5.97 log CFU/g, accounting for 1.1% of the mesophilic bacteria counts (7.94 log CFU/g). The psychrotrophic bacteria counts exceeded the counts of mesophilic bacteria for shrimp stored at 4 °C after 6 d of storage, indicating that psychrotrophic bacteria became predominant. The NGS was used to identify the bacterial species in samples stored at 25 and 4 °C. The results showed that the dominant microorganisms were Vibrio at 25 °C, and Acinetobacter, Psychrobacter, and Shewanella at 4 °C. By the culture-dependent method based on 16S rRNA gene and VITEK®2 CompactA system, it showed that the dominant microorganisms were Proteus spp. at 25 °C, and Shewanella putrefaciens, Acinetobacter johnsonii, and Aeromonas sobria at 4 °C. In conclusion, differences in results of microbiota analyzed by culture dependent and independent approaches were observed. The combination of both methodologies may provide more comprehensive information about the dominant spoilage microbiota in Pacific white shrimp during ambient and cold storage. © 2017 Institute of Food Technologists®.

  2. Preventing cold-related morbidity and mortality in a changing climate

    PubMed Central

    Conlon, Kathryn C; Rajkovich, Nicholas B; White-Newsome, Jalonne L; Larsen, Larissa; Neill, Marie S O

    2011-01-01

    Winter weather patterns are anticipated to become more variable with increasing average global temperatures. Research shows that excess morbidity and mortality occurs during cold weather periods. We critically reviewed evidence relating temperature variability, health outcomes, and adaptation strategies to cold weather. Health outcomes included cardiovascular-, respiratory-, cerebrovascular-, and all-cause morbidity and mortality. Individual and contextual risk factors were assessed to highlight associations between individual- and neighborhood- level characteristics that contribute to a person’s vulnerability to variability in cold weather events. Epidemiologic studies indicate that the populations most vulnerable to variations in cold winter weather are the elderly, rural and, generally, populations living in moderate winter climates. Fortunately, cold-related morbidity and mortality are preventable and strategies exist for protecting populations from these adverse health outcomes. We present a range of adaptation strategies that can be implemented at the individual, building, and neighborhood level to protect vulnerable populations from cold-related morbidity and mortality. The existing research justifies the need for increased outreach to individuals and communities for education on protective adaptations in cold weather. We propose that future climate change adaptation research couple building energy and thermal comfort models with epidemiological data to evaluate and quantify the impacts of adaptation strategies. PMID:21592693

  3. Marketing potential of advanced breeding clones

    USDA-ARS?s Scientific Manuscript database

    The accumulation of reducing sugars during cold storage of potato tubers is a serious and costly problem for producers and processors. The degree to which cultivars accumulate reducing sugars during storage determines their processing and market potential. Cultivars or advanced breeding lines with...

  4. Developing cold-chipping potato varieties by silencing the vacuolar invertase gene

    USDA-ARS?s Scientific Manuscript database

    Accumulation of reducing sugars during cold storage is a persistent and costly problem for the potato processing industry. High temperature processing of potato tubers with elevated amounts of reducing sugars results in potato chips, fries and other products that are unacceptable to consumers becaus...

  5. 75. VIEW OF SECOND WEAPONS STORAGE AREA IGLOO FIELD FROM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    75. VIEW OF SECOND WEAPONS STORAGE AREA IGLOO FIELD FROM ROOF OF BUILDING 328 LOOKING NORTHWEST SHOWING BUILDING 327-318. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  6. 44. NORTHWEST CORNER OF BUILDING 272 (STORAGE STRUCTURE A2) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    44. NORTHWEST CORNER OF BUILDING 272 (STORAGE STRUCTURE A-2) IN ASSEMBLY AREA SHOWING SHAPE OF EARTHEN BERM. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  7. Preliminary economic analysis of aquifer winter-chill storage at the John F. Kennedy airport

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fox, E.C.; Thomas, J.F.

    A conceptual design was formulated in conjuction with a cost analysis to determine the feasibility of retrofitting the present John F. Kennedy (JFK) airport air-conditioning system with an aquifer cold water storage system. It appears technically feasible to chill and store aquifer water at the airport site during the winter months for later air-conditioning use. However, the economic analysis shows that although a significant energy savings is realized, the money saved from reduced energy costs would not be enough to recover the necessary capital investment over a 20-year period. JFK airport may be a poor economic choice for an aquifermore » cold water storage demonstration site due to site specific problems, and other sites may provide economic incentive.« less

  8. Spent nuclear fuel canister storage building conceptual design report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Swenson, C.E.

    This Conceptual Design Report provides the technical basis for the Spent Nuclear Fuels Project, Canister Storage Building, and as amended by letter (correspondence number 9555700, M.E. Witherspoon to E.B. Sellers, ``Technical Baseline and Updated Cost Estimate for the Canister Storage Building``, dated October 24, 1995), includes the project cost baseline and Criteria to be used as the basis for starting detailed design in fiscal year 1995.

  9. Individual Shrink Wrapping of Zucchini Fruit Improves Postharvest Chilling Tolerance Associated with a Reduction in Ethylene Production and Oxidative Stress Metabolites.

    PubMed

    Megías, Zoraida; Martínez, Cecilia; Manzano, Susana; García, Alicia; Rebolloso-Fuentes, María Del Mar; Garrido, Dolores; Valenzuela, Juan Luis; Jamilena, Manuel

    2015-01-01

    We have studied the effect of individual shrink wrapping (ISW) on the postharvest performance of refrigerated fruit from two zucchini cultivars that differ in their sensitivity to cold storage: Sinatra (more sensitive) and Natura (more tolerant). The fruit was individually shrink wrapped before storing at 4°C for 0, 7 and 14 days. Quality parameters, ethylene and CO2 productions, ethylene gene expression, and oxidative stress metabolites were assessed in shrink wrapped and non-wrapped fruit after conditioning the fruit for 6 hours at 20°C. ISW decreased significantly the postharvest deterioration of chilled zucchini in both cultivars. Weight loss was reduced to less than 1%, pitting symptoms were completely absent in ISW fruit at 7 days, and were less than 25% those of control fruits at 14 days of cold storage, and firmness loss was significantly reduced in the cultivar Sinatra. These enhancements in quality of ISW fruit were associated with a significant reduction in cold-induced ethylene production, in the respiration rate, and in the level of oxidative stress metabolites such as hydrogen peroxide and malonyldialdehyde (MDA). A detailed expression analysis of ethylene biosynthesis, perception and signaling genes demonstrated a downregulation of CpACS1 and CpACO1 genes in response to ISW, two genes that are upregulated by cold storage. However, the expression patterns of six other ethylene biosynthesis genes (CpACS2 to CpACS7) and five ethylene signal transduction pathway genes (CpCTR1, CpETR1, CpERS1, CpEIN3.1 and CpEN3.2), suggest that they do not play a major role in response to cold storage and ISW packaging. In conclusion, ISW zucchini packaging resulted in improved tolerance to chilling concomitantly with a reduction in oxidative stress, respiration rate and ethylene production, as well as in the expression of ethylene biosynthesis genes, but not of those involved in ethylene perception and sensitivity.

  10. Individual Shrink Wrapping of Zucchini Fruit Improves Postharvest Chilling Tolerance Associated with a Reduction in Ethylene Production and Oxidative Stress Metabolites

    PubMed Central

    Megías, Zoraida; Martínez, Cecilia; Manzano, Susana; García, Alicia; Rebolloso-Fuentes, María del Mar; Garrido, Dolores; Valenzuela, Juan Luis; Jamilena, Manuel

    2015-01-01

    We have studied the effect of individual shrink wrapping (ISW) on the postharvest performance of refrigerated fruit from two zucchini cultivars that differ in their sensitivity to cold storage: Sinatra (more sensitive) and Natura (more tolerant). The fruit was individually shrink wrapped before storing at 4°C for 0, 7 and 14 days. Quality parameters, ethylene and CO2 productions, ethylene gene expression, and oxidative stress metabolites were assessed in shrink wrapped and non-wrapped fruit after conditioning the fruit for 6 hours at 20°C. ISW decreased significantly the postharvest deterioration of chilled zucchini in both cultivars. Weight loss was reduced to less than 1%, pitting symptoms were completely absent in ISW fruit at 7 days, and were less than 25% those of control fruits at 14 days of cold storage, and firmness loss was significantly reduced in the cultivar Sinatra. These enhancements in quality of ISW fruit were associated with a significant reduction in cold-induced ethylene production, in the respiration rate, and in the level of oxidative stress metabolites such as hydrogen peroxide and malonyldialdehyde (MDA). A detailed expression analysis of ethylene biosynthesis, perception and signaling genes demonstrated a downregulation of CpACS1 and CpACO1 genes in response to ISW, two genes that are upregulated by cold storage. However, the expression patterns of six other ethylene biosynthesis genes (CpACS2 to CpACS7) and five ethylene signal transduction pathway genes (CpCTR1, CpETR1, CpERS1, CpEIN3.1 and CpEN3.2), suggest that they do not play a major role in response to cold storage and ISW packaging. In conclusion, ISW zucchini packaging resulted in improved tolerance to chilling concomitantly with a reduction in oxidative stress, respiration rate and ethylene production, as well as in the expression of ethylene biosynthesis genes, but not of those involved in ethylene perception and sensitivity. PMID:26177024

  11. 3. PHOTOCOPY, ARCHITECTURAL AND STRUCTURAL PLAN, ELEVATIONS, AND SECTION DRAWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. PHOTOCOPY, ARCHITECTURAL AND STRUCTURAL PLAN, ELEVATIONS, AND SECTION DRAWING FOR WATER TREATMENT PUMPING AND STORAGE BUILDING. - NIKE Missile Base SL-40, Water Treatment & Storage Building, Southern portion of launch area, southeast of Ready Building, Hecker, Monroe County, IL

  12. 2. LAUNCH CONTROL SUPPORT BUILDING WEST FRONT AND VEHICLE STORAGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. LAUNCH CONTROL SUPPORT BUILDING WEST FRONT AND VEHICLE STORAGE BUILDING SOUTHWEST FRONT. VIEW TO EAST. - Minuteman III ICBM Launch Control Facility November-1, 1.5 miles North of New Raymer & State Highway 14, New Raymer, Weld County, CO

  13. Measurement of the Neutron Lifetime with Ultra-cold Neutrons Stored in a Magneto-gravitational Trap

    NASA Astrophysics Data System (ADS)

    Ezhov, V. F.; Andreev, A. Z.; Ban, G.; Bazarov, B. A.; Geltenbort, P.; Glushkov, A. G.; Knyazkov, V. A.; Kovrizhnykh, N. A.; Krygin, G. B.; Naviliat-Cuncic, O.; Ryabov, V. L.

    2018-05-01

    We report a measurement of the neutron lifetime using ultra-cold neutrons stored in a magneto-gravitational trap made of permanent magnets. Neutrons surviving in the trap after fixed storage times have been counted and the trap losses have continuously been monitored during storage by detecting neutrons leaking from the trap. The value of the neutron lifetime resulting from this measurement is τ n = (878.3 ± 1.6stat ± 1.0syst) s. A unique feature of this experiment is the monitoring of leaking neutrons providing a robust control of the main systematic loss.

  14. Seismic analyses of equipment in 2736-Z complex. Revision 1

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ocoma, E.C.

    1995-04-01

    This report documents the structural qualification for the existing equipment when subjected to seismic loading in the Plutonium Storage Complex. It replaces in entirety Revision 0 and reconciles the U.S. Department of Energy (DOE) comments on Revision 0. The Complex consists of 2736-Z Building (plutonium storage vault), 2736-ZA Building (vault ventilation equipment building), and 2736-ZB Building (shipping/receiving, repackaging activities). The existing equipment structurally qualified in this report are the metal storage racks for 7 inch and lard cans in room 2 of Building 2736-Z; the cubicles, can holders and pedestals in rooms 1, 3, and 4 of Building 2736-Z; themore » ventilation duct including exhaust fans/motors, emergency diesel generator, and HEPA filter housing in Building 2736-ZA; the repackaging glovebox in Building 2736-ZB; and the interface duct between Buildings 2736-Z and 2736-ZA.« less

  15. Fuel handling system for a nuclear reactor

    DOEpatents

    Saiveau, James G.; Kann, William J.; Burelbach, James P.

    1986-01-01

    A pool type nuclear fission reactor has a core, with a plurality of core elements and a redan which confines coolant as a hot pool at a first end of the core separated from a cold pool at a second end of the core by the redan. A fuel handling system for use with such reactors comprises a core element storage basket located outside of the redan in the cold pool. An access passage is formed in the redan with a gate for opening and closing the passage to maintain the temperature differential between the hot pool and the cold pool. A mechanism is provided for opening and closing the gate. A lifting arm is also provided for manipulating the fuel core elements through the access passage between the storage basket and the core when the redan gate is open.

  16. Fuel handling system for a nuclear reactor

    DOEpatents

    Saiveau, James G.; Kann, William J.; Burelbach, James P.

    1986-12-02

    A pool type nuclear fission reactor has a core, with a plurality of core elements and a redan which confines coolant as a hot pool at a first end of the core separated from a cold pool at a second end of the core by the redan. A fuel handling system for use with such reactors comprises a core element storage basket located outside of the redan in the cold pool. An access passage is formed in the redan with a gate for opening and closing the passage to maintain the temperature differential between the hot pool and the cold pool. A mechanism is provided for opening and closing the gate. A lifting arm is also provided for manipulating the fuel core elements through the access passage between the storage basket and the core when the redan gate is open.

  17. The effects of climate change on heating energy consumption of office buildings in different climate zones in China

    NASA Astrophysics Data System (ADS)

    Meng, Fanchao; Li, Mingcai; Cao, Jingfu; Li, Ji; Xiong, Mingming; Feng, Xiaomei; Ren, Guoyu

    2017-06-01

    Climate plays an important role in heating energy consumption owing to the direct relationship between space heating and changes in meteorological conditions. To quantify the impact, the Transient System Simulation Program software was used to simulate the heating loads of office buildings in Harbin, Tianjin, and Shanghai, representing three major climate zones (i.e., severe cold, cold, and hot summer and cold winter climate zones) in China during 1961-2010. Stepwise multiple linear regression was performed to determine the key climatic parameters influencing heating energy consumption. The results showed that dry bulb temperature (DBT) is the dominant climatic parameter affecting building heating loads in all three climate zones across China during the heating period at daily, monthly, and yearly scales (R 2 ≥ 0.86). With the continuous warming climate in winter over the past 50 years, heating loads decreased by 14.2, 7.2, and 7.1 W/m2 in Harbin, Tianjin, and Shanghai, respectively, indicating that the decreasing rate is more apparent in severe cold climate zone. When the DBT increases by 1 °C, the heating loads decrease by 253.1 W/m2 in Harbin, 177.2 W/m2 in Tianjin, and 126.4 W/m2 in Shanghai. These results suggest that the heating energy consumption can be well predicted by the regression models at different temporal scales in different climate conditions owing to the high determination coefficients. In addition, a greater decrease in heating energy consumption in northern severe cold and cold climate zones may efficiently promote the energy saving in these areas with high energy consumption for heating. Particularly, the likely future increase in temperatures should be considered in improving building energy efficiency.

  18. Postharvest conservation of the tuberous roots of Pachyrhizus Ahipa (Wedd) Parodi.

    PubMed

    Mussury, Rosilda M; Scalon, Silvana P Q; Silva, Magaiver A; Silva, Tatiane F; Gomes, Hellen; Gassi, Rosimeire

    2013-01-01

    This paper aimed to evaluate the effects of storage periods on the conservation of Pachyrhizus ahipa roots at different temperatures and packaging materials. The roots were harvested, washed, packed in PVC, plastic bags, without wrappings (control) and stored in polystyrene trays in refrigerators, or cold chambers, or at room temperature. Total titratable acidity (TTA), total soluble solids (TSS), pH, as well as their ash, lipid, total carbohydrate and protein (dry basis) contents were analyzed. The lowest loss of root fresh weight was observed in the cold chamber and plastic bags. The TTA remained higher among roots stored in the cold chamber and in PVC packaging. The lowest TSS contents were observed for roots stored in the cold chamber, and these did not vary among the packing materials. The average carbohydrate content percentage for all treatments was 84.9%. The percentage of lipids was highest in roots stored at room temperature while protein and ash contents were highest in roots under refrigeration. The best storage conditions for roots are plastic bags packaging in a cold chamber, with the roots retaining appropriate quality for commercialization for up to 30 days.

  19. Cold chain management in meat storage, distribution and retail: A review

    NASA Astrophysics Data System (ADS)

    Nastasijević, I.; Lakićević, B.; Petrović, Z.

    2017-09-01

    Meat is a perishable product with a short shelf life and therefore short selling times. Therefore, cold chain management in meat supply is of utmost importance for the maintenance of quality and safety of meat/meat products. Raw meat/meat products are likely to support the growth of pathogenic microorganisms and/or spoilage bacteria, and should be kept at temperatures that do not result in a risk to health. The cold chain should not be interrupted at all times along the meat distribution chain. The complexity of global meat supply chain, with frequently long distribution chains associated with transportation of the product within one country, from one to another country and from one to another continent, makes the solutions for the chilling and freezing regimes, as well as monitoring of time-temperature profiles, very important for the overall success in delivery of product which will be accepted by consumer for its freshness and safety levels. From recently, there are several available options for control and management of the cold chain, such as chilled and frozen storage combinations, superchilling, ionizing radiation, biopreservation, high hydrostatic pressure (HHP), active packaging, wireless sensors, supported with the software-based cold chain database (CCD).

  20. The effect of storage temperature and duration on northern red oak acorn viability and vigour

    Treesearch

    Thomas L. Noland; Andree E. Morneault; Daniel C. Dey; Dave Deugo

    2013-01-01

    Three separate collections of Ontario sources of northern red oak (Quercus rubra L.) acorns were made to determine the effects of long-term cold storage at +2°C, -1°C, and -2°C on their viability and vigour. We measured acorn moisture content, percent germination during storage, speed of germination and total germination...

  1. Evaluation of alternatives to fungicide to control postharvest gray mold alone or with ozone storage in grapes, 2011

    USDA-ARS?s Scientific Manuscript database

    Gray mold, caused by B. cinerea, causes severe losses since it spreads easily among berries during cold storage. Currently, it is controlled by fumigation with SO2 or SO2 emitting sheets within boxes. Alternative methods, such as storage in ozone atmospheres, are needed because SO2 is banned in orga...

  2. ‘Fuji’ apple (Malus domestica Borkh) volatile production during high pCO2 controlled atmosphere storage

    USDA-ARS?s Scientific Manuscript database

    ‘Fuji’apple [Malus sylvestris var. domestica (Borkh.) Mansf.] volatile compound dynamics were characterized during cold storage in air or at low pO2 controlled atmosphere (CA) with up to 5 kPa CO2. Volatile compounds in storage chambers were adsorbed onto solid sorbent traps and analyzed by GC-MS....

  3. Anthocyanin Concentration of “Assaria” Pomegranate Fruits During Different Cold Storage Conditions

    PubMed Central

    Antunes, Dulce

    2004-01-01

    The concentration of anthocyanins in fruits of “Assaria” pomegranate, a sweet Portuguese cultivar typically grown in Algarve (south Portugal), was monitored during storage under different conditions. The fruits were exposed to cold storage (5°C) after the following treatments: spraying with wax; spraying with 1.5% CaCl2; spraying with wax and 1.5% CaCl2; covering boxes with 25 μc thickness low-density polyethylene film. Untreated fruits were used as a control. The anthocyanin levels were quantified by either comparison with an external standard of cyanidin 3-rutinoside (based on the peak area) or individual calculation from the peak areas based on standard curves of each anthocyanin type. The storage time as well as the fruit treatment prior to storage influenced total anthocyanin content. The highest levels were observed at the end of the first month of storage, except for the fruits treated with CaCl2, where the maximal values were achieved at the end of the second month. The anthocyanin quantification method influenced the final result. When total anthocyanin was calculated as a sum of individual pigments quantified based on standard curves of each anthocyanin type, lower values were obtained. PMID:15577199

  4. 7 CFR 1436.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., dryers, processing plants, or cold storage facilities used for the storage and handling of any..., oats, wheat, barley, rice, raw or refined sugar, soybeans, sunflower seed, canola, rapeseed, safflower, flaxseed, mustard seed, crambe, sesame seed, other oilseeds as determined and announced by CCC, dry peas...

  5. 7 CFR 1436.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., dryers, processing plants, or cold storage facilities used for the storage and handling of any..., oats, wheat, barley, rice, raw or refined sugar, soybeans, sunflower seed, canola, rapeseed, safflower, flaxseed, mustard seed, crambe, sesame seed, other oilseeds as determined and announced by CCC, dry peas...

  6. 7 CFR 1436.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., dryers, processing plants, or cold storage facilities used for the storage and handling of any..., oats, wheat, barley, rice, raw or refined sugar, soybeans, sunflower seed, canola, rapeseed, safflower, flaxseed, mustard seed, crambe, sesame seed, other oilseeds as determined and announced by CCC, dry peas...

  7. 2. Missile transfer building, interior, transporter/erector on left, storage and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Missile transfer building, interior, transporter/erector on left, storage and shipping container, ballistic missile (SSCBM) containing minuteman II missile on right - Ellsworth Air Force Base, Delta Flight, Missile Roll Transfer Building, 920 Kennedy Road, Interior, Jackson County, SD

  8. 1. NORTH AND WEST SIDES OF BUILDING 728 FROM CHEMICAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. NORTH AND WEST SIDES OF BUILDING 728 FROM CHEMICAL STORAGE TANK. VIEW TO SOUTHEAST. - Rocky Mountain Arsenal, Mustard Filling & Storage Building, 280 feet South of December Seventh Avenue; 2130 feet East of D Street, Commerce City, Adams County, CO

  9. 18. CONTEXT VIEW LOOKING WEST OF BUILDING 345 (ENTRY CONTROL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. CONTEXT VIEW LOOKING WEST OF BUILDING 345 (ENTRY CONTROL BUILDING) IN STORAGE AREA WITH EAST LORING LAKE IN BACKGROUND. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  10. The Origins of the Cold War.

    ERIC Educational Resources Information Center

    Paterson, Thomas G.

    1986-01-01

    Briefly reviews conventional reasoning about the start of the Cold War. Describes contemporary revisionist views of the Cold War and the reasons they arose. Maintains that American leaders exaggerated the Soviet ideological and military threat, spurring an American arms build-up which ultimately led to the present-day arms race. (JDH)

  11. Improving the Processing Quality of Existing Cultivars by Suppressing the Vacuolar Acid Invertase Gene

    USDA-ARS?s Scientific Manuscript database

    Storing potato tubers at low temperatures is highly advantageous in that it prevents sprouting, minimizes disease losses and increases the marketing window. Unfortunately, cold storage of existing cultivars causes an unacceptable accumulation of reducing sugars, a phenomenon referred to as cold-indu...

  12. Pre-harvest application of polyamines enhances antioxidants and table grape (Vitis vinifera L.) quality during postharvest period.

    PubMed

    Mirdehghan, S H; Rahimi, S

    2016-04-01

    Pre-harvest foliar spraying of grapevines with putrescine (Put) and spermidine (Spd) (0, 1, 2mM) was evaluated for determining the fruit quality at harvest and improving postharvest characteristics of table grapes during cold storage. Fruit parameters in terms of firmness, fungal infection, weight loss, total phenol concentration, antioxidant activity, skin color, total anthocyanin concentration, total soluble solids (TSS) and titratable acidity (TA) were evaluated after 0, 25 and 55 days of storage at 1.5 ± 1 °C and 90 ± 5% R.H. Softening, fungal infection and weight loss increased during cold storage but the rate of changes significantly was delayed in Put- and Spd-treated fruits. Besides these, the application of Put and Spd maintained higher values of phenolics content, antioxidant activity and anthocyanins at the end of storage in compare to control. Furthermore, after 55 days of storage all treated fruits with Put and Spd showed lower changes in L(∗), TSS and TA and also higher value of chroma in berries. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. The influence of the dose of calcium bisglycinate on physicochemical properties, sensory analysis and texture profile of kefirs during 21 days of cold storage.

    PubMed

    Pawlos, Małgorzata; Znamirowska, Agata; Szajnar, Katarzyna; Kalicka, Dorota

    2016-01-01

    In the process of enrichment of dairy products a priority element is the proper selection of compounds that are a mineral carrier. Calcium bisglycinate is better absorbed by the body than inorganic forms of calcium. Moreover, the lactic acid which is produced in kefir fermentation and the presence of lactose have also a positive effect on the improvement of absorption of calcium. The aim of the present study was to determine the influence of the applied dose of calcium in the form of calcium bisglycinate on the physicochemical and sensory properties and texture of kefirs during 21-day period of cold storage. Processed cow milk was enriched with 0, 5, 10, 15, 20, 25 and 30 mg of calcium (for 100 g of milk), repasteurized (72°C, 1 min), cooled down (26°C), inoculated with Commercial VITAL kefir culture (Danisco, Poland) and fermented for 16 hours (26°C). The assessment of the influence of addition   of calcium bisglycinate on acidity, syneresis, texture and sensory characteristics (1-9 points) of kefirs was conducted at four fixed dates (after 1 day, 7 days, 14 days and 21 days of storage). During successive weeks of cold storage in all experimental groups there was observed a tendency to decrease general acidity. On the 1st and 7th days of cold storage reduced whey leakage was observed in kefirs enriched with 25 mg and 30 mg Ca/100 g of milk. With increasing doses of enrichment with calcium both the hardness, adhesiveness and gumminess of kefirs decreased. The applied doses of calcium did not cause changes in the sensory characteristics such as colour and consistency of the fermented beverages. Calcium bisglycinate may be used to enrich kefirs with calcium even with 30 mg of calcium in 100 g of milk without the modification of the product's parameters.

  14. 120. NORTH PLANT GB BULK STORAGE BUILDING AND AMMUNITION DEMOLITION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    120. NORTH PLANT GB BULK STORAGE BUILDING AND AMMUNITION DEMOLITION FACILITY AT CENTER AND CASE FILLING PLANT/CLUSTER ASSEMBLY BUILDING (BUILDING 1601/1606) IN BACKGROUND, FROM GB MANUFACTURING PLANT. VIEW TO WEST. - Rocky Mountain Arsenal, Bounded by Ninety-sixth Avenue & Fifty-sixth Avenue, Buckley Road, Quebec Street & Colorado Highway 2, Commerce City, Adams County, CO

  15. Vibrational spectroscopy for the evaluation of molecular perturbations induced in fruit lipids by cold storage

    NASA Astrophysics Data System (ADS)

    Bertoluzza, A.; Bottura, G.; Filippetti, P.; Tosi, M. R.; Vasina, M.; Pratella, G. C.; Folchi, A.; Gallerani, G.

    1994-07-01

    Vibrational spectroscopy (Raman, FT-IR-ATR) has been applied for the first time to the study of the mechanism of chilling stress and the monitoring of the best operative conditions for cold storage of fruit. In particular, this work deals with some results of the application of vibrational spectroscopy to the molecular characterization of lipidic extracts of fruits (apples and pears, pulp and peel) stored at low temperatures. The results have been obtained in a cooperative interdisciplinary research project performing experiments on fruits for one year cycles under different storage conditions of temperature (0°C, 8°C) and atmosphere (normal, controlled). The Raman spectra, useful for the evaluation of the transition temperature and the cooperative effect in the fruit membrane lipids, were masked by the strong resonance spectrum of carotenoids. The lipid unsaturation, the natural response to cold storage, was evaluated in the FT-IR-ATR spectra and expressed as the "total" unsaturation degree R = I{3012 cm -1}/{2858 cm -1}. The results on pulp and peel lipids have shown that the R value, higher in the pulps than peels, is dependent on the storage temperature and time. The increase in R is correlated with the higher fruit resistance to the chilling stress. Furthermore, the FT-IR spectra of the outer part of the fruits stored at 8°C show modifications of the carbonylic band at 1738 cm -1 (esteric group) such as the appearance of two other bands at 1715 and 1700 cm -1 increasing in intensity with storage time. These new components can be considered as molecular markers of the onset of a hydrolysis reaction and also of a partial peroxidation of the acylic unsaturated chains.

  16. Metabolomics evaluation of early-storage red blood cell rejuvenation at 4°C and 37°C.

    PubMed

    Gehrke, Sarah; Srinivasan, Amudan J; Culp-Hill, Rachel; Reisz, Julie A; Ansari, Andrea; Gray, Alan; Landrigan, Matthew; Welsby, Ian; D'Alessandro, Angelo

    2018-04-24

    Refrigerated red blood cell (RBC) storage results in the progressive accumulation of biochemical and morphological alterations collectively referred to as the storage lesion. Storage-induced metabolic alterations can be in part reversed by rejuvenation practices. However, rejuvenation requires an incubation step of RBCs for 1 hour at 37°C, limiting the practicality of providing "on-demand," rejuvenated RBCs. We tested the hypothesis that the addition of rejuvenation solution early in storage as an adjunct additive solution would prevent-in a time window consistent with the average age of units transfused to sickle cell recipients at Duke (15 days)-many of the adverse biochemical changes that can be reversed via standard rejuvenation, while obviating the incubation step. Metabolomics analyses were performed on cells and supernatants from AS-1 RBC units (n = 4), stored for 15 days. Units were split into pediatric bag aliquots and stored at 4°C. These were untreated controls, washed with or without rejuvenation, performed under either standard (37°C) or cold (4°C) conditions. All three treatments removed most metabolic storage by-products from RBC supernatants. However, only standard and cold rejuvenation provided significant metabolic benefits as judged by the reactivation of glycolysis and regeneration of adenosine triphosphate and 2,3-diphosphoglycerate. Improvements in energy metabolism also translated into increased capacity to restore the total glutathione pool and regenerate oxidized vitamin C in its reduced (ascorbate) form. Cold and standard rejuvenation of 15-day-old RBCs primes energy and redox metabolism of stored RBCs, while providing a logistic advantage for routine blood bank processing workflows. © 2018 AABB.

  17. A&M. A&M building (TAN607). Camera facing east. From left to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    A&M. A&M building (TAN-607). Camera facing east. From left to right, pool section, hot shop, cold shop, and machine shop. Biparting doors to hot shop are in open position behind shroud. Four rail tracks lead to hot shop and cold shop. Date: August 20, 1954. INEEL negative no. 11706 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

  18. VIEW NORTH OF PRESTRESS TRACK CENTERHEMP STORAGE BUILDING 77 (1920) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW NORTH OF PRE-STRESS TRACK CENTER-HEMP STORAGE BUILDING 77 (1920) ROPE WAREHOUSE 43 (1941) BEHIND IT STORAGE SHED 44 (1953) IN FRONT - John A. Roebling's Sons Company & American Steel & Wire Company, South Broad, Clark, Elmer, Mott & Hudson Streets, Trenton, Mercer County, NJ

  19. A&M. Radioactive parts security storage warehouses: TAN648 on left, and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    A&M. Radioactive parts security storage warehouses: TAN-648 on left, and dolly storage building, TAN-647, on right. Camera facing south. This was the front entry for the warehouse and the rear of the dolly storage building. Date: August 6, 2003. INEEL negative no. HD-36-2-2 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

  20. Performances of different global positioning system devices for time-location tracking in air pollution epidemiological studies.

    PubMed

    Wu, Jun; Jiang, Chengsheng; Liu, Zhen; Houston, Douglas; Jaimes, Guillermo; McConnell, Rob

    2010-11-23

    People's time-location patterns are important in air pollution exposure assessment because pollution levels may vary considerably by location. A growing number of studies are using global positioning systems (GPS) to track people's time-location patterns. Many portable GPS units that archive location are commercially available at a cost that makes their use feasible for epidemiological studies. We evaluated the performance of five portable GPS data loggers and two GPS cell phones by examining positional accuracy in typical locations (indoor, outdoor, in-vehicle) and factors that influence satellite reception (building material, building type), acquisition time (cold and warm start), battery life, and adequacy of memory for data storage. We examined stationary locations (eg, indoor, outdoor) and mobile environments (eg, walking, traveling by vehicle or bus) and compared GPS locations to highly-resolved US Geological Survey (USGS) and Digital Orthophoto Quarter Quadrangle (DOQQ) maps. The battery life of our tested instruments ranged from <9 hours to 48 hours. The acquisition of location time after startup ranged from a few seconds to >20 minutes and varied significantly by building structure type and by cold or warm start. No GPS device was found to have consistently superior performance with regard to spatial accuracy and signal loss. At fixed outdoor locations, 65%-95% of GPS points fell within 20-m of the corresponding DOQQ locations for all the devices. At fixed indoor locations, 50%-80% of GPS points fell within 20-m of the corresponding DOQQ locations for all the devices except one. Most of the GPS devices performed well during commuting on a freeway, with >80% of points within 10-m of the DOQQ route, but the performance was significantly impacted by surrounding structures on surface streets in highly urbanized areas. All the tested GPS devices had limitations, but we identified several devices which showed promising performance for tracking subjects' time location patterns in epidemiological studies.

  1. Performances of Different Global Positioning System Devices for Time-Location Tracking in Air Pollution Epidemiological Studies

    PubMed Central

    Wu, Jun; Jiang, Chengsheng; Liu, Zhen; Houston, Douglas; Jaimes, Guillermo; McConnell, Rob

    2010-01-01

    Background: People’s time-location patterns are important in air pollution exposure assessment because pollution levels may vary considerably by location. A growing number of studies are using global positioning systems (GPS) to track people’s time-location patterns. Many portable GPS units that archive location are commercially available at a cost that makes their use feasible for epidemiological studies. Methods: We evaluated the performance of five portable GPS data loggers and two GPS cell phones by examining positional accuracy in typical locations (indoor, outdoor, in-vehicle) and factors that influence satellite reception (building material, building type), acquisition time (cold and warm start), battery life, and adequacy of memory for data storage. We examined stationary locations (eg, indoor, outdoor) and mobile environments (eg, walking, traveling by vehicle or bus) and compared GPS locations to highly-resolved US Geological Survey (USGS) and Digital Orthophoto Quarter Quadrangle (DOQQ) maps. Results: The battery life of our tested instruments ranged from <9 hours to 48 hours. The acquisition of location time after startup ranged from a few seconds to >20 minutes and varied significantly by building structure type and by cold or warm start. No GPS device was found to have consistently superior performance with regard to spatial accuracy and signal loss. At fixed outdoor locations, 65%–95% of GPS points fell within 20-m of the corresponding DOQQ locations for all the devices. At fixed indoor locations, 50%–80% of GPS points fell within 20-m of the corresponding DOQQ locations for all the devices except one. Most of the GPS devices performed well during commuting on a freeway, with >80% of points within 10-m of the DOQQ route, but the performance was significantly impacted by surrounding structures on surface streets in highly urbanized areas. Conclusions: All the tested GPS devices had limitations, but we identified several devices which showed promising performance for tracking subjects’ time location patterns in epidemiological studies. PMID:21151593

  2. Double-image storage optimized by cross-phase modulation in a cold atomic system

    NASA Astrophysics Data System (ADS)

    Qiu, Tianhui; Xie, Min

    2017-09-01

    A tripod-type cold atomic system driven by double-probe fields and a coupling field is explored to store double images based on the electromagnetically induced transparency (EIT). During the storage time, an intensity-dependent signal field is applied further to extend the system with the fifth level involved, then the cross-phase modulation is introduced for coherently manipulating the stored images. Both analytical analysis and numerical simulation clearly demonstrate a tunable phase shift with low nonlinear absorption can be imprinted on the stored images, which effectively can improve the visibility of the reconstructed images. The phase shift and the energy retrieving rate of the probe fields are immune to the coupling intensity and the atomic optical density. The proposed scheme can easily be extended to the simultaneous storage of multiple images. This work may be exploited toward the end of EIT-based multiple-image storage devices for all-optical classical and quantum information processings.

  3. GENERAL VIEW LOOKING NORTHEAST FROM ATOP A STORAGE TANK, LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    GENERAL VIEW LOOKING NORTHEAST FROM ATOP A STORAGE TANK, LOOKING AT THE CATALYZER BUILDINGS. NOTE CIRCULAR FOUNDATION FOR AMMONIA STORAGE TANK AND THE LIQUID AIR BUILDING IN THE UPPPER RIGHT CORNER OF PHOTO. - United States Nitrate Plant No. 2, Reservation Road, Muscle Shoals, Muscle Shoals, Colbert County, AL

  4. 8. Interior view of former food service and storage area ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Interior view of former food service and storage area looking towards hall way; showing closed and open doorways to walk-in storage; near southwest corner of building on main floor; view to southwest. - Ellsworth Air Force Base, Mess & Administration Building, 1561 Ellsworth Street, Blackhawk, Meade County, SD

  5. Subcontracted activities related to TES for building heating and cooling

    NASA Technical Reports Server (NTRS)

    Martin, J.

    1980-01-01

    The subcontract program elements related to thermal energy storage for building heating and cooling systems are outlined. The following factors are included: subcontracts in the utility load management application area; life and stability testing of packaged low cost energy storage materials; and development of thermal energy storage systems for residential space cooling. Resistance storage heater component development, demonstration of storage heater systems for residential applications, and simulation and evaluation of latent heat thermal energy storage (heat pump systems) are also discussed. Application of thermal energy storage for solar application and twin cities district heating are covered including an application analysis and technology assessment of thermal energy storage.

  6. Characterization of injury in isolated rat proximal tubules during cold incubation and rewarming.

    PubMed

    Bienholz, Anja; Walter, Björn; Pless-Petig, Gesine; Guberina, Hana; Kribben, Andreas; Witzke, Oliver; Rauen, Ursula

    2017-01-01

    Organ shortage leads to an increased utilization of marginal organs which are particularly sensitive to storage-associated damage. Cold incubation and rewarming-induced injury is iron-dependent in many cell types. In addition, a chloride-dependent component of injury has been described. This work examines the injury induced by cold incubation and rewarming in isolated rat renal proximal tubules. The tissue storage solution TiProtec® and a chloride-poor modification, each with and without iron chelators, were used for cold incubation. Incubation was performed 4°C for up to 168 h, followed by rewarming in an extracellular buffer (3 h at 37°C). After 48, 120 and 168 h of cold incubation LDH release was lower in solutions containing iron chelators. After rewarming, injury increased especially after cold incubation in chelator-free solutions. Without addition of iron chelators LDH release showed a tendency to be higher in chloride-poor solutions. Following rewarming after 48 h of cold incubation lipid peroxidation was significantly decreased and metabolic activity was tendentially better in tubules incubated with iron chelators. Morphological alterations included mitochondrial swelling and fragmentation being partially reversible during rewarming. ATP content was better preserved in chloride-rich solutions. During rewarming, there was a further decline of ATP content in the so far best conditions and minor alterations under the other conditions, while oxygen consumption was not significantly different compared to non-stored control tubules. Results show an iron-dependent component of preservation injury during cold incubation and rewarming in rat proximal renal tubules and reveal a benefit of chloride for the maintenance of tubular energy state during cold incubation.

  7. Characterization of injury in isolated rat proximal tubules during cold incubation and rewarming

    PubMed Central

    Bienholz, Anja; Walter, Björn; Pless-Petig, Gesine; Guberina, Hana; Kribben, Andreas; Witzke, Oliver; Rauen, Ursula

    2017-01-01

    Organ shortage leads to an increased utilization of marginal organs which are particularly sensitive to storage-associated damage. Cold incubation and rewarming-induced injury is iron-dependent in many cell types. In addition, a chloride-dependent component of injury has been described. This work examines the injury induced by cold incubation and rewarming in isolated rat renal proximal tubules. The tissue storage solution TiProtec® and a chloride-poor modification, each with and without iron chelators, were used for cold incubation. Incubation was performed 4°C for up to 168 h, followed by rewarming in an extracellular buffer (3 h at 37°C). After 48, 120 and 168 h of cold incubation LDH release was lower in solutions containing iron chelators. After rewarming, injury increased especially after cold incubation in chelator-free solutions. Without addition of iron chelators LDH release showed a tendency to be higher in chloride-poor solutions. Following rewarming after 48 h of cold incubation lipid peroxidation was significantly decreased and metabolic activity was tendentially better in tubules incubated with iron chelators. Morphological alterations included mitochondrial swelling and fragmentation being partially reversible during rewarming. ATP content was better preserved in chloride-rich solutions. During rewarming, there was a further decline of ATP content in the so far best conditions and minor alterations under the other conditions, while oxygen consumption was not significantly different compared to non-stored control tubules. Results show an iron-dependent component of preservation injury during cold incubation and rewarming in rat proximal renal tubules and reveal a benefit of chloride for the maintenance of tubular energy state during cold incubation. PMID:28672023

  8. 31. Perimeter acquisition radar building room #318, data storage "racks"; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. Perimeter acquisition radar building room #318, data storage "racks"; sign read: M&D controller, logic control buffer, data transmission controller - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

  9. Biochemical and proteomic analysis of grape berries (Vitis labruscana) during cold storage upon postharvest salicylic acid treatment.

    PubMed

    Cai, Han; Yuan, Xiaozhuan; Pan, Jiaojiao; Li, Huan; Wu, Ziming; Wang, Yun

    2014-10-15

    Salicylic acid (SA) treatment has been widely used to maintain fruit quality during postharvest storage. To elucidate the molecular mechanism related to this treatment, the effect of SA treatment on fruit quality as well as protein expression profiles of grape berries (Vitis labruscana cv. Kyoho) during the subsequent cold storage was evaluated. As expected, SA treatment inhibited postharvest loss and chilling damage by reducing fruit softening and membrane damage and slowing weight loss. A gel-based proteomic approach was designed to screen for differentially expressed proteins in SA-treated and control grape berries. A total of 69 differentially accumulated proteins were successfully identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, which can be functionally classified into eight categories. Among these proteins, antioxidant enzymes including ascorbate peroxidase, oxidoreductase, and glutathione S-transferase were induced, and the abundances of several defense-related proteins, such as heat shock protein (HSP) and temperature-induced lipocalin, were up-regulated by SA treatment. In addition, proteins involved in carbohydrate catabolism and energy production were also induced by SA treatment. Interpretation of the data for differential accumulation of proteins revealed that the effect of SA on reducing postharvest losses and chilling damage of grape berries during cold storage may be due to activated defense responses and carbohydrate metabolism and higher levels of energy status.

  10. The effect of clove bud powder at a spice level on antioxidant and quality properties of emulsified pork sausage during cold storage.

    PubMed

    Jin, Sang-Keun; Choi, Jung-Seok; Jeong, Jin-Yeon; Kim, Gap-Don

    2016-09-01

    Clove bud is a widely used spice in meat and meat products, and it contains high level of phenolic compounds. The effectiveness of the clove as a spice has not been fully studied at a general level of addition in the meat products. Therefore, in the present study, the antioxidant, antimicrobial, and nitrite scavenging abilities of clove bud powder (CBP) was assessed at spice level (0.1% and 0.2%) in emulsified pork sausage, during 6 weeks of cold storage. CBP had DPPH radical scavenging ability, but CBP addition at 0.1% and 0.2% did not decrease the TBARS value. An antimicrobial effect of CBP was also not observed during the cold storage. However, residual nitrite at storage weeks 4 and 6 was shown to be lower (P < 0.05). Addition of CBP decreased CIE L* and a* values, but it produced unacceptable sensory properties. Texture profile analysis was not affected by the addition of CBP in emulsified pork sausage (P > 0.05). The positive effect on nitrite scavenging could be expected by the addition of 0.2% CBP as a spice. However, antioxidant and antimicrobial abilities were not observed, as well as improvement in the quality of characteristics, in emulsified pork sausage. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  11. Induction of antioxidant flavonol biosynthesis in fresh-cut potatoes. Effect of domestic cooking.

    PubMed

    Tudela, Juan A; Cantos, Emma; Espín, Juan C; Tomás-Barberán, Francisco A; Gil, María I

    2002-10-09

    The effect of fresh-cutting and subsequent cold storage on phenolic compounds from five long-term-stored potato cultivars (Agria, Cara, Liseta, Monalisa, and Spunta) was studied. Fresh-cutting induced the biosynthesis of three flavonols, which were identified by HPLC-DAD-ESIMS as quercetin 3-rutinoside, quercetin 3-diglucoside, and quercetin 3-glucosylrutinoside. The flavonols were detected after a lag period of 3 days of cold storage. The content ranged from 6 to 14 mg/100 g of fresh weight depending on the cultivar after 6 days of storage. Chlorogenic acid as the main caffeic acid derivative and the amino acids tyrosine and tryptophan were also quantified. The effect of cold storage under light or in dark was studied with new-season-harvested Monalisa potatoes. The flavonol induction was higher in fresh-cut potatoes stored under light than in the dark. However, caffeic acid derivatives were not affected. Domestic cooking such as boiling, microwaving, and frying provoked a partial loss of the flavonols, which were retained in the range of 4-16 mg per serving (213 g). Steam-cooking resulted in the highest retention of caffeic acid derivatives and aromatic amino acids compared with the other cooking methods studied. This means that due to the large amount of potatoes consumed in the Western diet, fresh-cut potatoes can be a significant source of health-promoting phenolics.

  12. Simplified numerical description of latent storage characteristics for phase change wallboard

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Feustel, H.E.

    1995-05-01

    Cooling of residential California buildings contributes significantly to electrical consumption and peak power demand. Thermal mass can be utilized to reduce the peak-power demand, down-size the cooling systems and/or switch to low-energy cooling sources. Large thermal storage devices have been used in the past to overcome the short-comings of alternative cooling sources or to avoid high demand charges. With the advent of phase change material (PCM) implemented in gypsum board, plaster or other wall-covering material, thermal storage can be part of the building structure even for light-weight buildings. PCMs have two important advantages as storage media: they can offer anmore » order-of-magnitude increase in thermal storage capacity and their discharge is almost isothermal. This allows to store large amounts of energy without significantly changing the temperature of the sheathing. As heat storage takes place in the building part where the loads occur, rather than externally (e.g., ice or chilled water storage), additional transport energy is not needed. To numerically evaluate the latent storage performance of treated wallboard, RADCOOL, a thermal building simulation model based on the finite difference approach, will be used. RADCOOL has been developed in the SPARK environment in order to be compatible with the new family of simulation tools being developed at Lawrence Berkeley Laboratory. As logical statements are difficult to use in SPARK, a continuous function for the specific heat and the enthalpy had to be found. This report covers the development of a simplified description of latent storage characteristics for wallboard treated with phase change material.« less

  13. 46. ARAI. Aerial view of ARAI buildings as they looked ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    46. ARA-I. Aerial view of ARA-I buildings as they looked in 1981. From left to right, buildings are tank (ARA-727), contaminated waste storage tank (ARA-629), trailer, hot cell building (ARA-626), fuel oil storage tank (ARA-728), guard house (ARA-628), shop and maintenance building (ARA-627), and two trailers. Ineel photo no. 81-297. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID

  14. Augmenting Transport versus Increasing Cold Storage to Improve Vaccine Supply Chains

    PubMed Central

    Haidari, Leila A.; Connor, Diana L.; Wateska, Angela R.; Brown, Shawn T.; Mueller, Leslie E.; Norman, Bryan A.; Schmitz, Michelle M.; Paul, Proma; Rajgopal, Jayant; Welling, Joel S.; Leonard, Jim; Chen, Sheng-I; Lee, Bruce Y.

    2013-01-01

    Background When addressing the urgent task of improving vaccine supply chains, especially to accommodate the introduction of new vaccines, there is often a heavy emphasis on stationary storage. Currently, donations to vaccine supply chains occur largely in the form of storage equipment. Methods This study utilized a HERMES-generated detailed, dynamic, discrete event simulation model of the Niger vaccine supply chain to compare the impacts on vaccine availability of adding stationary cold storage versus transport capacity at different levels and to determine whether adding stationary storage capacity alone would be enough to relieve potential bottlenecks when pneumococcal and rotavirus vaccines are introduced by 2015. Results Relieving regional level storage bottlenecks increased vaccine availability (by 4%) more than relieving storage bottlenecks at the district (1% increase), central (no change), and clinic (no change) levels alone. Increasing transport frequency (or capacity) yielded far greater gains (e.g., 15% increase in vaccine availability when doubling transport frequency to the district level and 18% when tripling). In fact, relieving all stationary storage constraints could only increase vaccine availability by 11%, whereas doubling the transport frequency throughout the system led to a 26% increase and tripling the frequency led to a 30% increase. Increasing transport frequency also reduced the amount of stationary storage space needed in the supply chain. The supply chain required an additional 61,269L of storage to relieve constraints with the current transport frequency, 55,255L with transport frequency doubled, and 51,791L with transport frequency tripled. Conclusions When evaluating vaccine supply chains, it is important to understand the interplay between stationary storage and transport. The HERMES-generated dynamic simulation model showed how augmenting transport can result in greater gains than only augmenting stationary storage and can reduce stationary storage needs. PMID:23717590

  15. Augmenting transport versus increasing cold storage to improve vaccine supply chains.

    PubMed

    Haidari, Leila A; Connor, Diana L; Wateska, Angela R; Brown, Shawn T; Mueller, Leslie E; Norman, Bryan A; Schmitz, Michelle M; Paul, Proma; Rajgopal, Jayant; Welling, Joel S; Leonard, Jim; Chen, Sheng-I; Lee, Bruce Y

    2013-01-01

    When addressing the urgent task of improving vaccine supply chains, especially to accommodate the introduction of new vaccines, there is often a heavy emphasis on stationary storage. Currently, donations to vaccine supply chains occur largely in the form of storage equipment. This study utilized a HERMES-generated detailed, dynamic, discrete event simulation model of the Niger vaccine supply chain to compare the impacts on vaccine availability of adding stationary cold storage versus transport capacity at different levels and to determine whether adding stationary storage capacity alone would be enough to relieve potential bottlenecks when pneumococcal and rotavirus vaccines are introduced by 2015. Relieving regional level storage bottlenecks increased vaccine availability (by 4%) more than relieving storage bottlenecks at the district (1% increase), central (no change), and clinic (no change) levels alone. Increasing transport frequency (or capacity) yielded far greater gains (e.g., 15% increase in vaccine availability when doubling transport frequency to the district level and 18% when tripling). In fact, relieving all stationary storage constraints could only increase vaccine availability by 11%, whereas doubling the transport frequency throughout the system led to a 26% increase and tripling the frequency led to a 30% increase. Increasing transport frequency also reduced the amount of stationary storage space needed in the supply chain. The supply chain required an additional 61,269L of storage to relieve constraints with the current transport frequency, 55,255L with transport frequency doubled, and 51,791L with transport frequency tripled. When evaluating vaccine supply chains, it is important to understand the interplay between stationary storage and transport. The HERMES-generated dynamic simulation model showed how augmenting transport can result in greater gains than only augmenting stationary storage and can reduce stationary storage needs.

  16. Response of aboveground carbon balance to long-term, experimental enhancements in precipitation seasonality is contingent on plant community type in cold-desert rangelands

    USGS Publications Warehouse

    McAbee, Kathryn; Reinhardt, Keith; Germino, Matthew; Bosworth, Andrew

    2017-01-01

    Semi-arid rangelands are important carbon (C) pools at global scales. However, the degree of net C storage or release in water-limited systems is a function of precipitation amount and timing, as well as plant community composition. In northern latitudes of western North America, C storage in cold-desert ecosystems could increase with boosts in wintertime precipitation, in which climate models predict, due to increases in wintertime soil water storage that enhance summertime productivity. However, there are few long-term, manipulative field-based studies investigating how rangelands will respond to altered precipitation amount or timing. We measured aboveground C pools and fluxes at leaf, soil, and ecosystem scales over a single growing season in plots that had 200 mm of supplemental precipitation added in either winter or summer for the past 21 years, in shrub- and exotic-bunchgrass-dominated garden plots. At our cold-desert site (298 mm precipitation during the study year), we hypothesized that increased winter precipitation would stimulate the aboveground C uptake and storage relative to ambient conditions, especially in plots containing shrubs. Our hypotheses were generally supported: ecosystem C uptake and long-term biomass accumulation were greater in winter- and summer-irrigated plots compared to control plots in both vegetation communities. However, substantial increases in the aboveground biomass occurred only in winter-irrigated plots that contained shrubs. Our findings suggest that increases in winter precipitation will enhance C storage of this widespread ecosystem, and moreso in shrub- compared to grass-dominated communities.

  17. Response of aboveground carbon balance to long-term, experimental enhancements in precipitation seasonality is contingent on plant community type in cold-desert rangelands.

    PubMed

    McAbee, Kathryn; Reinhardt, Keith; Germino, Matthew J; Bosworth, Andrew

    2017-03-01

    Semi-arid rangelands are important carbon (C) pools at global scales. However, the degree of net C storage or release in water-limited systems is a function of precipitation amount and timing, as well as plant community composition. In northern latitudes of western North America, C storage in cold-desert ecosystems could increase with boosts in wintertime precipitation, in which climate models predict, due to increases in wintertime soil water storage that enhance summertime productivity. However, there are few long-term, manipulative field-based studies investigating how rangelands will respond to altered precipitation amount or timing. We measured aboveground C pools and fluxes at leaf, soil, and ecosystem scales over a single growing season in plots that had 200 mm of supplemental precipitation added in either winter or summer for the past 21 years, in shrub- and exotic-bunchgrass-dominated garden plots. At our cold-desert site (298 mm precipitation during the study year), we hypothesized that increased winter precipitation would stimulate the aboveground C uptake and storage relative to ambient conditions, especially in plots containing shrubs. Our hypotheses were generally supported: ecosystem C uptake and long-term biomass accumulation were greater in winter- and summer-irrigated plots compared to control plots in both vegetation communities. However, substantial increases in the aboveground biomass occurred only in winter-irrigated plots that contained shrubs. Our findings suggest that increases in winter precipitation will enhance C storage of this widespread ecosystem, and moreso in shrub- compared to grass-dominated communities.

  18. Cold air drainage flows subsidize montane valley ecosystem productivity

    Treesearch

    Kimberly A. Novick; Andrew C. Oishi; Chelcy Ford Miniat

    2016-01-01

    In mountainous areas, cold air drainage from high to low elevations has pronounced effects on local temperature, which is a critical driver of many ecosystem processes, including carbon uptake and storage. Here, we leverage new approaches for interpreting ecosystem carbon flux observations in complex terrain to quantify the links between macro-climate...

  19. Innovative cold tolerance test for conifer seedlings

    Treesearch

    Peter A. Balk; Peter Bronnum; Mike Perks; Eva Stattin; Lonneke H. M. van der Geest; Monique F. van Wordragen

    2007-01-01

    Forest tree nurseries rely on tight scheduling of operations to deliver vital seedlings to the planting site. Cold storage is required to: (1) prevent winter damage, especially in container seedlings; (2) to maintain planting stock in an inactive condition; and (3) to ensure plant supply for geographically distinct planting sites, a definite requirement for large-scale...

  20. Environmental prevalence and persistence of Listeria monocytogenes in cold-smoked trout processing plants

    USDA-ARS?s Scientific Manuscript database

    The presence of Listeria monocytogenes on the surfaces of equipment and workers' hands during different production stages, as well as on fish skin and meat during processing and storage of cold-smoked trout, was investigated. Listeria monocytogenes was recovered from 10 (6.06%) of a total 165 cotto...

  1. Research of the cold shield in cryogenic liquid storage

    NASA Astrophysics Data System (ADS)

    Chen, L. B.; Zheng, J. P.; Wu, X. L.; Cui, C.; Zhou, Y.; Wang, J. J.

    2017-12-01

    To realize zero boil-off storage of cryogenic liquids, a cryocooler that can achieve a temperature below the boiling point temperature of the cryogenic liquid is generally needed. Taking into account that the efficiency of the cryocooler will be higher at a higher operating temperature, a novel thermal insulation system using a sandwich container filled with cryogenic liquid with a higher boiling point as a cold radiation shield between the cryogenic tank and the vacuum shield in room temperature is proposed to reduce the electricity power consumption. A two-stage cryocooler or two separate cryocoolers are adopted to condense the evaporated gas from the cold shield and the cryogenic tank. The calculation result of a 55 liter liquid hydrogen tank with a liquid nitrogen shield shows that only 14.4 W of electrical power is needed to make all the evaporated gas condensation while 121.7 W will be needed without the liquid nitrogen shield.

  2. Feasibility study for a Cryogenic On-Orbit Liquid Depot-Storage, Acquisition and Transfer (COLD-SAT) satellite

    NASA Technical Reports Server (NTRS)

    Rybak, S. C.; Willen, G. S.; Follett, W. H.; Hanna, G. J.; Cady, E. C.; Distefano, E.; Meserole, J. S.

    1990-01-01

    This feasibility study presents the conceptual design of a spacecraft for performing a series of cryogenic fluid management flight experiments. This spacecraft, the Cryogenic On-Orbit Liquid Depot-Storage, Acquisition, and Transfer (COLD-SAT) satellite, will use liquid hydrogen as the test fluid, be launched on a Delta expendable launch vehicle, and conduct a series of experiments over a two to three month period. These experiments will investigate the physics of subcritical cryogens in the low gravity space environment to characterize their behavior and to correlate the data with analytical and numerical models of in-space cryogenic fluid management systems. Primary technologies addressed by COLD-SAT are: (1) pressure control; (2) chilldown; (3) no-vent fill; (4) liquid acquisition device fill; (5) pressurization; (6) low-g fill and drain; (7) liquid acquisition device expulsion; (8) line chilldown; (9) thermodynamic state control; and (10) fluid dumping.

  3. 39. Perimeter acquisition radar building room #504, techinal maintenance and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. Perimeter acquisition radar building room #504, techinal maintenance and repair center (TMRC) and tactical support equipment (TSE) storage area; storage-travel wave tubes - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

  4. 46 CFR 525.1 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ship's tackle. (11) Heavy lift means the service of providing heavy lift cranes and equipment for lifting cargo. (12) Loading and unloading means the service of loading or unloading cargo between any... storage spaces, cold storage plants, cranes, grain elevators and/or bulk cargo loading and/or unloading...

  5. 46 CFR 525.1 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., warehouse or other terminal facilities in connection with a common carrier, or in connection with a common...; common carriers who perform port terminal services; and warehousemen who operate port terminal facilities... storage spaces, cold storage plants, cranes, grain elevators and/or bulk cargo loading and/or unloading...

  6. Maintenance of host DNA integrity in field-preserved mosquito (Diptera: Culicidae) blood meals for identification by DNA barcoding.

    PubMed

    Reeves, Lawrence E; Holderman, Chris J; Gillett-Kaufman, Jennifer L; Kawahara, Akito Y; Kaufman, Phillip E

    2016-09-15

    Determination of the interactions between hematophagous arthropods and their hosts is a necessary component to understanding the transmission dynamics of arthropod-vectored pathogens. Current molecular methods to identify hosts of blood-fed arthropods require the preservation of host DNA to serve as an amplification template. During transportation to the laboratory and storage prior to molecular analysis, genetic samples need to be protected from nucleases, and the degradation effects of hydrolysis, oxidation and radiation. Preservation of host DNA contained in field-collected blood-fed specimens has an additional caveat: suspension of the degradative effects of arthropod digestion on host DNA. Unless effective preservation methods are implemented promptly after blood-fed specimens are collected, host DNA will continue to degrade. Preservation methods vary in their efficacy, and need to be selected based on the logistical constraints of the research program. We compared four preservation methods (cold storage at -20 °C, desiccation, ethanol storage of intact mosquito specimens and crushed specimens on filter paper) for field storage of host DNA from blood-fed mosquitoes across a range of storage and post-feeding time periods. The efficacy of these techniques in maintaining host DNA integrity was evaluated using a polymerase chain reaction (PCR) to detect the presence of a sufficient concentration of intact host DNA templates for blood meal analysis. We applied a logistic regression model to assess the effects of preservation method, storage time and post-feeding time on the binomial response variable, amplification success. Preservation method, storage time and post-feeding time all significantly impacted PCR amplification success. Filter papers and, to a lesser extent, 95 % ethanol, were the most effective methods for the maintenance of host DNA templates. Amplification success of host DNA preserved in cold storage at -20 °C and desiccation was poor. Our data suggest that, of the methods tested, host DNA template integrity was most stable when blood meals were preserved using filter papers. Filter paper preservation is effective over short- and long-term storage, while ethanol preservation is only suitable for short-term storage. Cold storage at -20 °C, and desiccation of blood meal specimens, even for short time periods, should be avoided.

  7. Bacterial membranes: the effects of chill storage and food processing. An overview.

    PubMed

    Russell, Nicholas J

    2002-11-15

    The shelf life of food is extended by refrigeration because the metabolic processes of food-associated microorganisms are slowed by the lowered temperature. Nonetheless, cold-adapted psychrotrophic food-poisoning and food-spoilage bacteria remain a concern because they possess cold-adapted proteins and membrane lipids that facilitate growth at low temperatures. The use of membrane-disrupting novel preservation techniques, such as ultrasound, high hydrostatic pressure or pulsed electric field, offer the potential for an extension of shelf life. This review considers the interacting and potentially synergistic effects of chill storage or mild heat treatment on membrane properties, with the disruptive effects of membrane-targeted physical treatments.

  8. Preliminary thermal design of the COLD-SAT spacecraft

    NASA Technical Reports Server (NTRS)

    Arif, Hugh

    1991-01-01

    The COLD-SAT free-flying spacecraft was to perform experiments with LH2 in the cryogenic fluid management technologies of storage, supply and transfer in reduced gravity. The Phase A preliminary design of the Thermal Control Subsystem (TCS) for the spacecraft exterior and interior surfaces and components of the bus subsystems is described. The TCS was composed of passive elements which were augmented with heaters. Trade studies to minimize the parasitic heat leakage into the cryogen storage tanks are described. Selection procedure for the thermally optimum on-orbit spacecraft attitude was defined. TRASYS-2 and SINDA'85 verification analysis was performed on the design and the results are presented.

  9. Building heating and cooling applications thermal energy storage program overview

    NASA Technical Reports Server (NTRS)

    Eissenberg, D. M.

    1980-01-01

    Thermal energy storage technology and development of building heating and cooling applications in the residential and commercial sectors is outlined. Three elements are identified to undergo an applications assessment, technology development, and demonstration. Emphasis is given to utility load management thermal energy system application where the stress is on the 'customer side of the meter'. Thermal storage subsystems for space conditioning and conservation means of increased thermal mass within the building envelope and by means of low-grade waste heat recovery are covered.

  10. Resource Conservation and Recovery Act (RCRA) Part B Permit Application for Production Associated Units at the Oak Ridge Y-12 Plant

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    This is the RCRA required permit application for Radioactive and Hazardous Waste Management at the Oak Ridge Y-12 Plant for the following units: Building 9206 Container Storage Unit; Building 9212 Container Storage Unit; Building 9720-12 Container Storage Unit; Cyanide Treatment Unit. All four of these units are associated with the recovery of enriched uranium and other metals from wastes generated during the processing of nuclear materials.

  11. Building and managing high performance, scalable, commodity mass storage systems

    NASA Technical Reports Server (NTRS)

    Lekashman, John

    1998-01-01

    The NAS Systems Division has recently embarked on a significant new way of handling the mass storage problem. One of the basic goals of this new development are to build systems at very large capacity and high performance, yet have the advantages of commodity products. The central design philosophy is to build storage systems the way the Internet was built. Competitive, survivable, expandable, and wide open. The thrust of this paper is to describe the motivation for this effort, what we mean by commodity mass storage, what the implications are for a facility that performs such an action, and where we think it will lead.

  12. Seasonal Thermal Energy Storage Program

    NASA Technical Reports Server (NTRS)

    Minor, J. E.

    1980-01-01

    The Seasonal Thermal Energy Storage (STES) Program designed to demonstrate the storage and retrieval of energy on a seasonal basis using heat or cold available from waste or other sources during a surplus period is described. Factors considered include reduction of peak period demand and electric utility load problems and establishment of favorable economics for district heating and cooling systems for commercialization of the technology. The initial thrust of the STES Program toward utilization of ground water systems (aquifers) for thermal energy storage is emphasized.

  13. Vaccine storage and cold chain monitoring in the North West region of Cameroon: a cross sectional study.

    PubMed

    Yakum, Martin Ndinakie; Ateudjieu, Jerome; Walter, Ebile Akoh; Watcho, Pierre

    2015-04-14

    The cold chain must be monitored continuously in order to guarantee vaccines' quality. From field reports and previous studies, cold chain monitoring for expanded program on immunization (EPI) is still not satisfactory in Cameroon. This study was conducted to evaluate the availability and functioning of cold chain equipment as well as knowledge. It was a cross-sectional study involving a multistage sampling. 3urban and 5rural districts were selected randomly from the 19 health districts of the North West region. In each district all the health facilities taking part in the EPI were targeted. Data were collected using a questionnaire administered face to face to health personnel and with an observational grid to assess availability, functioning, and monitoring of cold chain equipment and power supply. The data were analyzed using the epi-info software. A total of 70 health facilities were contacted and 65(88.6%) of them included in the study. Fifty-three (81.5%) out of 65 health facilities had at least one functional vaccine refrigerator. The national guideline of EPI was not present in 21(33.9%) health facilities. Temperature chart was complete/correctly filled in 25(50.0%) of the 50(96.2%) facilities having it. About 14 (26.9%) of the health facilities record at least one abnormal temperature during the last 2 months following data collection. Seventeen (28.3%) personnel did not know the correct vaccine storage temperature. The availability of vaccine storage equipment for EPI is acceptable in the North West Region of Cameroon but the capacity of those in charge to properly monitor it in all health facilities is still limited. To ensure that vaccines administered in the North West Region are stored at the recommended temperature, all District Health Services should train and regularly supervise the health personnel in charge of cold chain monitoring.

  14. Final Environmental Assessment: Base-Wide Building Demolition Arnold Air Force Base, Tennessee

    DTIC Science & Technology

    2006-02-01

    Building • Engine Test Facility ( ETF )-B Exhauster • ETF -A Airside • ETF -A Exhauster • ETF -A Reefer • CE Facility • Rocket Storage • Von Karman Gas...Executive Order ESA Endangered Species Act ETF Engine Test Facility FamCamp Family Camping Area P:\\ARNOLDAFB\\333402DO42COMPLIANCE\\DEMOLITION...Fabrication Shop • Natural Resources Building • Salt Storage Building • Administration Building • Engine Test Facility ( ETF )-B Exhauster • ETF -A

  15. Selection efficiency for raspberry postharvest shelf life affected by storage temperature and harvest season

    USDA-ARS?s Scientific Manuscript database

    Improved postharvest quality is an important goal for fresh-market raspberry breeding programs. To determine if warm or cold storage following harvest would better facilitate the breeding selection process for the assessment of postharvest decay and bleed, pesticide-free fruit from cultivars and bre...

  16. Medium-term in vitro storage as a complementary germplasm preservation technique

    USDA-ARS?s Scientific Manuscript database

    A Germplasm preservation of vegetatively propagated crops may be accomplished using a range of old and new technologies. Field collections, potted plants, or some cases whole plants stored under cool to cold conditions are complemented by in vitro culture, in vitro storage, and cryopreservation. The...

  17. Cold temperature delays wound healing in postharvest sugarbeet roots

    USDA-ARS?s Scientific Manuscript database

    Storage temperature affects the rate and extent of wound-healing in a number of root and tuber crops. The effect of storage temperature on wound-healing in sugarbeet (Beta vulgaris L.) roots, however, is largely unknown. Wound-healing of sugarbeet roots was investigated using surface-abraded roots s...

  18. Potential long-term storage of the predatory mite Phytoseiulus persimilis

    USDA-ARS?s Scientific Manuscript database

    Increasing the ability to store mass-reared natural enemies during periods or seasons of low demand is a critical need of the biocontrol industry. We tested the hypothesis that cryoprotectant or carbohydrate molecules can enhance long-term cold storage of a predatory mite Phytoseiulus persimilis At...

  19. Phase-change wallboard and mechanical night ventilation in commercial buildings: Potential for HVAC system downsizing

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stetiu, C.; Feustel, H.E.

    1998-07-01

    As thermal storage media, phase-change materials (PCMs) such as paraffin, eutectic salts, etc. offer an order-of-magnitude increase in thermal storage capacity, and their discharge is almost isothermal. By embedding PCMs in dypsum board, plaster, or other wall-covering materials, the building structure acquires latent storage properties. Structural elements containing PCMs can store large amounts of energy while maintaining the indoor temperature within a relatively narrow range. As heat storage takes place inside the building where the loads occur, rather than at a central exterior location, the internal loads are removed without the need for additional transport energy. Distributed latent storage canmore » thus be used to reduce the peak power demand of a building, downsize the cooling system, and/or switch to low-energy cooling sources. The authors used RADCOOL, a thermal building simulation program based on the finite difference approach, to numerically evaluate the thermal performance of PCM wallboard coupled with mechanical night ventilation in office buildings offers the opportunity for system downsizing in climates where the outside air temperature drops below 18 C at night. In climates where the outside air temperature remains above 19 C at night, the use of PCM wallboard should be coupled with discharge mechanisms other than mechanical night ventilation with outside air.« less

  20. KENNEDY SPACE CENTER, FLA. - Workers in the Columbia Debris Hangar pull items from storage containers to transfer to storage in the Vehicle Assembly Building. About 83,000 pieces were shipped to KSC during search and recovery efforts in East Texas.

    NASA Image and Video Library

    2003-09-02

    KENNEDY SPACE CENTER, FLA. - Workers in the Columbia Debris Hangar pull items from storage containers to transfer to storage in the Vehicle Assembly Building. About 83,000 pieces were shipped to KSC during search and recovery efforts in East Texas.

  1. Effects of ethrel, 1-MCP and modified atmosphere packaging on the quality of 'Wonderful' pomegranates during cold storage.

    PubMed

    Valdenegro, Mónika; Huidobro, Camila; Monsalve, Liliam; Bernales, Maricarmen; Fuentes, Lida; Simpson, Ricardo

    2018-03-24

    Pomegranate (Punica granatum) is a non-climacteric fruit susceptible to chilling injury (CI) at temperatures below 5 °C. To understand the influences of ethylene and modified atmosphere on CI physiological disorders of pomegranate, exogenous ethrel (0.5, 1 and 1.5 µg L -1 ) treatments, 1-methylcyclopropene (1-MCP) (1 µL L -1 ) exposure, packaging in a modified atmosphere (MAP) (XTend™ bags; StePac, São Paulo, Brazil), a MAP/1-MCP combination, and packaging in macro-perforated bags (MPB) were applied. The treated fruits were cold stored (2 ± 1 °C; 85% relative humidity) and sampled during 120 + 3 days at 20 °C. During cold storage, CI symptoms started at 20 days in MPB and at 60 days for all exogenous ethylene treatments, and were delayed to 120 days in MAP, 1-MCP and MAP/1-MCP treatments. MPB and ethylene treatments induced significant electrolyte leakage, oxidative damage, lipid peroxidation, ethylene and CO 2 production, and 1-aminocyclopropane-1-carboxylic acid oxidase activity, without any change in total soluble solids, titratable acidity or skin and aril colours. Conversely, MAP by itself, or in combination with 1-MCP application, effectively delayed CI symptoms. During long-term cold storage of this non-climacteric fruit, ethrel application induced endogenous ethylene biosynthesis, accelerating the appearance of CI symptoms in contrast to the observations made for MAP and 1-MCP treatments. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.

  2. 64. VIEW OF EAST LORING LAKE LOOKING WEST FROM HILL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    64. VIEW OF EAST LORING LAKE LOOKING WEST FROM HILL BEHIND BUILDING 345 (ENTRY CONTROL BUILDING) IN STORAGE AREA, WITH BUILDING 1026 (BASE SPARES AREA WATER TOWER) IN DISTANCE. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  3. Experimental test of a hot water storage system including a macro-encapsulated phase change material (PCM)

    NASA Astrophysics Data System (ADS)

    Mongibello, L.; Atrigna, M.; Bianco, N.; Di Somma, M.; Graditi, G.; Risi, N.

    2017-01-01

    Thermal energy storage systems (TESs) are of fundamental importance for many energetic systems, essentially because they permit a certain degree of decoupling between the heat or cold production and the use of the heat or cold produced. In the last years, many works have analysed the addition of a PCM inside a hot water storage tank, as it can allow a reduction of the size of the storage tank due to the possibility of storing thermal energy as latent heat, and as a consequence its cost and encumbrance. The present work focuses on experimental tests realized by means of an indoor facility in order to analyse the dynamic behaviour of a hot water storage tank including PCM modules during a charging phase. A commercial bio-based PCM has been used for the purpose, with a melting temperature of 58°C. The experimental results relative to the hot water tank including the PCM modules are presented in terms of temporal evolution of the axial temperature profile, heat transfer and stored energy, and are compared with the ones obtained by using only water as energy storage material. Interesting insights, relative to the estimation of the percentage of melted PCM at the end of the experimental test, are presented and discussed.

  4. Storage at low temperature differentially affects the colour and carotenoid composition of two cultivars of banana.

    PubMed

    Facundo, Heliofabia Virginia De Vasconcelos; Gurak, Poliana Deyse; Mercadante, Adriana Zerlotti; Lajolo, Franco Maria; Cordenunsi, Beatriz Rosana

    2015-03-01

    Different storage conditions can induce changes in the colour and carotenoid profiles and levels in some fruits. The goal of this work was to evaluate the influence of low temperature storage on the colour and carotenoid synthesis in two banana cultivars: Prata and Nanicão. For this purpose, the carotenoids from the banana pulp were determined by HPLC-DAD-MS/MS, and the colour of the banana skin was determined by a colorimeter method. Ten carotenoids were identified, of which the major carotenoids were all-trans-lutein, all-trans-α-carotene and all-trans-β-carotene in both cultivars. The effect of the low temperatures was subjected to linear regression analysis. In cv. Prata, all-trans-α-carotene and all-trans-β-carotene were significantly affected by low temperature (p<0.01), with negative estimated values (β coefficients) indicating that during cold storage conditions, the concentrations of these carotenoids tended to decrease. In cv. Nanicão, no carotenoid was significantly affected by cold storage (p>0.05). The accumulation of carotenoids in this group may be because the metabolic pathways using these carotenoids were affected by storage at low temperatures. The colour of the fruits was not negatively affected by the low temperatures (p>0.05). Published by Elsevier Ltd.

  5. Retrofit of a Multifamily Mass Masonry Building in New England

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ueno, K.; Kerrigan, P.; Wytrykowska, H.

    2013-08-01

    Merrimack Valley Habitat for Humanity (MVHfH) has partnered with Building Science Corporation to provide high performance affordable housing for 10 families in the retrofit of an existing brick building (a former convent) into condominiums. The research performed for this project provides information regarding advanced retrofit packages for multi-family masonry buildings in Cold climates. In particular, this project demonstrates safe, durable, and cost-effective solutions that will potentially benefit millions of multi-family brick buildings throughout the East Coast and Midwest (Cold climates). The retrofit packages provide insight on the opportunities for and constraints on retrofitting multifamily buildings with ambitious energy performance goalsmore » but a limited budget. The condominium conversion project will contribute to several areas of research on enclosures, space conditioning, and water heating. Enclosure items include insulation of mass masonry building on the interior, airtightness of these types of retrofits, multi-unit building compartmentalization, window selection, and roof insulation strategies. Mechanical system items include combined hydronic and space heating systems with hydronic distribution in small (low load) units, and ventilation system retrofits for multifamily buildings.« less

  6. Retrofit of a MultiFamily Mass Masonry Building in New England

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ueno, K.; Kerrigan, P.; Wytrykowska, H.

    2013-08-01

    Merrimack Valley Habitat for Humanity (MVHfH) has partnered with Building Science Corporation to provide high performance affordable housing for 10 families in the retrofit of an existing brick building (a former convent) into condominiums. The research performed for this project provides information regarding advanced retrofit packages for multi-family masonry buildings in Cold climates. In particular, this project demonstrates safe, durable, and cost-effective solutions that will potentially benefit millions of multi-family brick buildings throughout the East Coast and Midwest (Cold climates). The retrofit packages provide insight on the opportunities for and constraints on retrofitting multifamily buildings with ambitious energy performance goalsmore » but a limited budget. The condominium conversion project will contribute to several areas of research on enclosures, space conditioning, and water heating. Enclosure items include insulation of mass masonry building on the interior, airtightness of these types of retrofits, multi-unit building compartmentalization, window selection, and roof insulation strategies. Mechanical system items include combined hydronic and space heating systems with hydronic distribution in small (low load) units, and ventilation system retrofits for multifamily buildings.« less

  7. Microbiological Aspects of Geothermal Energy: Influence of Microbial Activity on Scaling and Clogging in a Cold Storage

    NASA Astrophysics Data System (ADS)

    Lerm, Stephanie; Alawi, Mashal; Miethling-Graff, Rona; Vieth, Andrea; Seibt, Andrea; Wolfgramm, Markus; Würdemann, Hilke

    2010-05-01

    The development of strategies to substantially reduce emission of greenhouse gases to the atmosphere is one of the major challenges of the next decades. Therefore, the utilization of subsurface stored energy arouses increasing interest. Corrosion and scaling are major problems in geothermal operation which create significant maintenance and cleaning costs. In the scope of the research project AquiScreen the operational reliability of geothermal used aquifer systems was investigated under microbial, geochemical, mineralogical, and petrologic aspects (see also Alawi et al.; General Assembly EGU 2010). This presentation focuses on the investigation of a cold storage in Berlin (Reichstag building, depth 30-50 m). In order to evaluate the impact of microbial processes in the low saline aquifer (see also Vetter et al.; General Assembly EGU 2010), the microbial communities of fluid and filter samples were investigated by Fluorescent in situ hybridization (FISH) and DNA fingerprinting techniques based on PCR amplified partial 16S rRNA genes. Analyses of fluid samples revealed a bacterial community dominated by iron and sulfur oxidizing bacteria closely related to Siderooxidans lithoautotrophicus, Gallionella sp. and Thiotrix unzii. Scanning electron microscope analysis revealed iron hydroxide formation and precipitation in the filter of the top side facility and the well, corresponding to the abundance of iron oxidizing bacteria. Besides oxidizing bacteria sulfate reducing bacteria (SRB) were detected as well, indicating the formation of micro-habitats with divergent redox zones. After several years of operation and routine maintenance procedures the injectivity of the injection wells and the endurance of the top side facility filters were reduced drastically due to clogging. Mechanical cleaning and a disinfection treatment with hydrogen peroxide (H2O2) were successful to re-establish the injectivity of the wells. The results of the microbiological investigations prove that bacteria and their metabolic activities were involved in the decrease of filter endurances. A strong biofilm formation of filamentous sulfur-oxidizing bacteria related to Thiothrix was observed. In the course of the disinfection measure the microbial composition in the process water changed significantly. Thiothrix could not be detected any longer and the biocoenosis in the fluid was dominated now by Flavobacterium, Acidovorax as well as Alcaligenaceae related organisms. In contrast, SRB analyzed by specific dissimilatory sulfite reductase genes were hardly affected by the disinfection measures. However, even if especially SRB are considered as the most important taxonomic group for microbiologically influenced corrosion (MIC), present operational results indicate that scaling and clogging were the predominant processes for the operation of the shallow cold storage in Berlin.

  8. INTERIOR VIEW OF FUEL STORAGE BUILDING (CPP603) LOOKING SOUTHWEST SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR VIEW OF FUEL STORAGE BUILDING (CPP-603) LOOKING SOUTHWEST SHOWING STORAGE BASIN IN FOREGROUND, TRANSFER CRANE AND UNLOADER TO LEFT OF NORTH SIDE OF HOT CELL. INL PHOTO NUMBER NRTS-58-157. J. Anderson, Photographer, 1/15/1958 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  9. Transcriptomic analysis of fruit stored under cold conditions using controlled atmosphere in Prunus persica cv. "Red Pearl".

    PubMed

    Sanhueza, Dayan; Vizoso, Paula; Balic, Iván; Campos-Vargas, Reinaldo; Meneses, Claudio

    2015-01-01

    Cold storage (CS) can induce a physiological disorder known as chilling injury (CI) in nectarine fruits. The main symptom is mealiness that is perceived as non-juicy fruit by consumers. Postharvest treatments such as controlled atmosphere (CA; a high CO2 concentration and low O2) have been used under cold conditions to avoid this disorder. With the objective of exploring the mechanisms involved in the CA effect on mealiness prevention, we analyzed transcriptomic changes under six conditions of "Red Pearl" nectarines by RNA-Seq. Our analysis included just harvested nectarines, juicy non-stored fruits, fruits affected for CI after CS and fruits stored in a combination of CA plus CS without CI phenotype. Nectarines stored in cold conditions combined with CA treatment resulted in less mealiness; we obtained 21.6% of juice content compared with just CS fruits (7.7%; mealy flesh). RNA-Seq data analyses were carried out to study the gene expression for different conditions assayed. During ripening, we detected that nectarines exposed to CA treatment expressed a similar number of genes compared with fruits that were not exposed to cold conditions. Firm fruits have more differentially expressed genes than soft fruits, which suggest that most important changes occur during CS. On the other hand, gene ontology analysis revealed enrichment mainly in metabolic and cellular processes. Differentially expressed genes analysis showed that low O2 concentrations combined with cold conditions slows the metabolic processes more than just the cold storage, resulting mainly in the suppression of primary metabolism and cold stress response. This is a significant step toward unraveling the molecular mechanism that explains the effectiveness of CA as a tool to prevent CI development on fruits.

  10. Transcriptomic analysis of fruit stored under cold conditions using controlled atmosphere in Prunus persica cv. “Red Pearl”

    PubMed Central

    Sanhueza, Dayan; Vizoso, Paula; Balic, Iván; Campos-Vargas, Reinaldo; Meneses, Claudio

    2015-01-01

    Cold storage (CS) can induce a physiological disorder known as chilling injury (CI) in nectarine fruits. The main symptom is mealiness that is perceived as non-juicy fruit by consumers. Postharvest treatments such as controlled atmosphere (CA; a high CO2 concentration and low O2) have been used under cold conditions to avoid this disorder. With the objective of exploring the mechanisms involved in the CA effect on mealiness prevention, we analyzed transcriptomic changes under six conditions of “Red Pearl” nectarines by RNA-Seq. Our analysis included just harvested nectarines, juicy non-stored fruits, fruits affected for CI after CS and fruits stored in a combination of CA plus CS without CI phenotype. Nectarines stored in cold conditions combined with CA treatment resulted in less mealiness; we obtained 21.6% of juice content compared with just CS fruits (7.7%; mealy flesh). RNA-Seq data analyses were carried out to study the gene expression for different conditions assayed. During ripening, we detected that nectarines exposed to CA treatment expressed a similar number of genes compared with fruits that were not exposed to cold conditions. Firm fruits have more differentially expressed genes than soft fruits, which suggest that most important changes occur during CS. On the other hand, gene ontology analysis revealed enrichment mainly in metabolic and cellular processes. Differentially expressed genes analysis showed that low O2 concentrations combined with cold conditions slows the metabolic processes more than just the cold storage, resulting mainly in the suppression of primary metabolism and cold stress response. This is a significant step toward unraveling the molecular mechanism that explains the effectiveness of CA as a tool to prevent CI development on fruits. PMID:26483806

  11. Genetic stability of ectomycorrhizal fungi is not affected by cryopreservation at -130 °C or cold storage with repeated sub-cultivations over a period of 2 years.

    PubMed

    Crahay, Charlotte; Munaut, Françoise; Colpaert, Jan V; Huret, Stéphanie; Declerck, Stéphane

    2017-08-01

    Cryopreservation is considered the most reliable method for storage of filamentous fungi including ectomycorrhizal (ECM) fungi. A number of studies, however, have reported genetic changes in fungus cultures following cryopreservation. In the present study, the genetic stability of six ECM fungus isolates was analyzed using amplified fragment length polymorphism (AFLP). The isolates were preserved for 2 years either by cryopreservation (at -130 °C) or by storage at 4 °C with regular sub-cultivation. A third preservation treatment consisting of isolates maintained on Petri dishes at 22-23 °C for 2 years (i.e., without any sub-cultivation) was included and used as a control. The differences observed in AFLP patterns between the three preservation methods remained within the range of the total error generated by the AFLP procedure (6.85%). Therefore, cryopreservation at -130 °C and cold storage with regular sub-cultivation did not affect the genetic stability of the ECM fungus isolates, and both methods can be used for the routine storage of ECM fungus isolates over a period of 2 years.

  12. Measuring Device for Air Speed in Macroporous Media and Its Application Inside Apple Storage Bins.

    PubMed

    Geyer, Martin; Praeger, Ulrike; Truppel, Ingo; Scaar, Holger; Neuwald, Daniel A; Jedermann, Reiner; Gottschalk, Klaus

    2018-02-13

    In cold storage facilities of fruit and vegetables, airflow is necessary for heat removal. The design of storage facilities influences the air speed in the surrounding of the product. Therefore, knowledge about airflow next to the product is important to plan the layout of cold stores adapted to the requirements of the products. A new sensing device (ASL, Air speed logger) is developed for omnidirectional measurement of air speed between fruit or vegetables inside storage bins or in bulk. It consists of four interconnected plastic spheres with 80 mm diameter each, adapted to the size of apple fruit. In the free space between the spheres, silicon diodes are fixed for the airflow measurement based on a calorimetric principle. Battery and data logger are mounted inside the spheres. The device is calibrated in a wind tunnel in a measuring range of 0-1.3 m/s. Air speed measurements in fruit bulks on laboratory scale and in an industrial fruit store show air speeds in gaps between fruit with high stability at different airflow levels. Several devices can be placed between stored products for determination of the air speed distribution inside bulks or bin stacks in a storage room.

  13. Cell wall, cell membrane, and volatile metabolism are altered by antioxidant treatment, temperature shifts, and peel necrosis during apple fruit storage.

    PubMed

    Leisso, Rachel; Buchanan, David; Lee, Jinwook; Mattheis, James; Rudell, David

    2013-02-13

    The transition from cold storage to ambient temperature alters apple quality through accelerated softening, flavor and color changes, and development of physiological peel disorders, such as superficial scald, in susceptible cultivars. To reveal global metabolism associated with this transition, the 'Granny Smith' peel metabolome was evaluated during storage of 6 months and shelf life periods. Treatment with the antioxidant diphenylamine (DPA) reduced scald, creating a metabolic contrast with untreated fruit, which developed superficial scald. Superficial scald symptoms developed on control fruit after 120 days of storage, and symptoms progressed following transition to ambient-temperature shelf life. The metabolic profile of control and DPA-treated fruit was divergent after 30 days of cold storage due to differing levels of α-farnesene oxidation products, methyl esters, phytosterols, and other compounds potentially associated with chloroplast integrity and oxidative stress response. Hierarchical cluster analysis revealed coregulation within the volatile synthesis pathway including control of the availability of methyl, propyl, ethyl, acetyl, and butyl alcohol and/or acid moieties for ester biosynthesis. Overall, the application of metabolomics techniques lends new insight into physiological processes leading to cell death and ripening processes that affect fruit flavor, appearance, and overall quality.

  14. An optimized formulation of a thermostable spray dried virus-like particles vaccine against human papillomavirus

    PubMed Central

    Saboo, Sugandha; Tumban, Ebenezer; Peabody, Julianne; Wafula, Denis; Peabody, David S.; Chackerian, Bryce; Muttil, Pavan

    2016-01-01

    Existing vaccines against human papillomavirus (HPV) require continuous cold-chain storage. Previously, we developed a bacteriophage virus-like particle (VLP) based vaccine for Human Papillomavirus (HPV) infection, which elicits broadly neutralizing antibodies against diverse HPV types. Here, we formulated these VLPs into a thermostable dry powder using a multi-component excipient system and by optimizing the spray drying parameters using a half-factorial design approach. Dry powder VLPs were stable after spray drying and after long-term storage at elevated temperatures. Immunization of mice with a single dose of reconstituted dry powder VLPs that were stored at 37°C for more than a year elicited high anti-L2 IgG antibody titers. Spray dried thermostable, broadly protective L2 bacteriophage VLPs vaccine could be accessible to remote regions of the world (where ~84% of cervical cancer patients reside) by eliminating the cold-chain requirement during transportation and storage. PMID:27019231

  15. Assessment of radioisotope heaters for remote terrestrial applications

    NASA Astrophysics Data System (ADS)

    Uherka, Kenneth L.

    This paper examines the feasibility of using radioisotope byproducts for special heating applications at remote sites in Alaska and other cold regions. The investigation included assessment of candidate radioisotope materials for heater applications, identification of the most promising cold-region applications, evaluation of key technical issues and implementation constraints, and development of conceptual heater designs for candidate applications. Strontium-90 (Sr-90) was selected as the most viable fuel for radioisotopic heaters used in terrestrial applications. Opportunities for the application of radioisotopic heaters were determined through site visits to representative Alaskan installations. Candidate heater applications included water storage tanks, sludge digesters, sewage lagoons, water piping systems, well-head pumping stations, emergency shelters, and fuel storage tank deicers. Radio-isotopic heaters for freeze-up protection of water storage tanks and for enhancement of biological waste treatment processes at remote sites were selected as the most promising applications.

  16. Effects of chronic N fertilization on foliar membranes, cold tolerance, and carbon storage in montane red spruce

    Treesearch

    Paul G. Schaberg; Donald H. DeHayes; Gary J. Hawley; Paula F. Murakami; G. Richard Strimbeck; Steven G. McNulty

    2002-01-01

    We evaluated the influence of protracted low-level nitrogen (N) fertilization on foliar membrane-associated calcium (mCa), sugar and starch concentrations, membrane stability, winter cold tolerance, and freezing injury of red spruce (Picea rubens Sarg.) trees growing in six experimental plots on Mount Ascutney, Vermont. For 12 consecutive years...

  17. Influence of packaging on the quality of cold-stored grapes packed into boxes for later repacking

    USDA-ARS?s Scientific Manuscript database

    A two-year study was conducted to examine various commercial practices associated with the cold storage of table grapes that are to be later re-packed for final shipment to provide information on the impact on fruit quality. Variables examined included the use of box types with vent areas ranging f...

  18. Credit BG. View looks south (174°) at Deluge Water Supply ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Credit BG. View looks south (174°) at Deluge Water Supply complex, including Water Pumping Booster Station (Building 4317) in foreground, with Deluge Water Storage (Building 4316) in background at right. Pole on roof of Building 4316 is a gauge board used to indicate water level in the reservoir. Structure in left background is Building 4311 (Well No. 2) - Edwards Air Force Base, North Base, Deluge Water Storage Building, Near Second & D Streets, Boron, Kern County, CA

  19. Blood banking-induced alteration of red blood cell oxygen release ability

    PubMed Central

    Li, Yaojin; Xiong, Yanlian; Wang, Ruofeng; Tang, Fuzhou; Wang, Xiang

    2016-01-01

    Background Current blood banking procedures may not fully preserve red blood cell (RBC) function during storage, contributing to the decrease of RBC oxygen release ability. This study was undertaken to evaluate the impact of routine cold storage on RBC oxygen release ability. Materials and methods RBC units were collected from healthy donors and each unit was split into two parts (whole blood and suspended RBC) to exclude possible donor variability. Oxygen dissociation measurements were performed on blood units stored at 4 °C during a 5-week period. 2,3-diphosphoglycerate levels and fluorescent micrographs of erythrocyte band 3 were also analysed. Results P50 and oxygen release capacity decreased rapidly during the first 3 weeks, and then did not change significantly. In contrast, the kinetic properties (PO2-t curve and T*50) of oxygen release changed slowly during the first 3 weeks of storage, but then decreased significantly in the last 2 weeks. 2,3-diphosphoglycerate decreased quickly during the first 3 weeks of storage to almost undetectable levels. Band 3 aggregated significantly during the last 2 weeks of storage. Discussion RBC oxygen release ability appears to be sensitive to routine cold storage. The thermodynamic characteristics of RBC oxygen release ability changed mainly in the first 3 weeks of storage, due to the decrease of 2,3-diphosphoglycerate, whereas the kinetic characteristics of RBC oxygen release ability decreased significantly at the end of storage, probably affected by alterations of band 3. PMID:26674824

  20. Blood banking-induced alteration of red blood cell oxygen release ability.

    PubMed

    Li, Yaojin; Xiong, Yanlian; Wang, Ruofeng; Tang, Fuzhou; Wang, Xiang

    2016-05-01

    Current blood banking procedures may not fully preserve red blood cell (RBC) function during storage, contributing to the decrease of RBC oxygen release ability. This study was undertaken to evaluate the impact of routine cold storage on RBC oxygen release ability. RBC units were collected from healthy donors and each unit was split into two parts (whole blood and suspended RBC) to exclude possible donor variability. Oxygen dissociation measurements were performed on blood units stored at 4 °C during a 5-week period. 2,3-diphosphoglycerate levels and fluorescent micrographs of erythrocyte band 3 were also analysed. P50 and oxygen release capacity decreased rapidly during the first 3 weeks, and then did not change significantly. In contrast, the kinetic properties (PO2-t curve and T*50) of oxygen release changed slowly during the first 3 weeks of storage, but then decreased significantly in the last 2 weeks. 2,3-diphosphoglycerate decreased quickly during the first 3 weeks of storage to almost undetectable levels. Band 3 aggregated significantly during the last 2 weeks of storage. RBC oxygen release ability appears to be sensitive to routine cold storage. The thermodynamic characteristics of RBC oxygen release ability changed mainly in the first 3 weeks of storage, due to the decrease of 2,3-diphosphoglycerate, whereas the kinetic characteristics of RBC oxygen release ability decreased significantly at the end of storage, probably affected by alterations of band 3.

  1. Analysis on energy-saving path of rural buildings in hot summer and cold winter zone

    NASA Astrophysics Data System (ADS)

    Huang, Mingqiang; Li, Jinheng

    2018-02-01

    Since the reform and opening policy, the construction of rural area in China has become more and more important. The idea of establishing green villages needs to be accepted and recognized by the public. The hot summer and cold winter zone combines two contradictory weather conditions that is cold winter and hot summer. So the living conditions are limited. In response to this climate, residents extensively use electric heaters or air conditioning to adjust the indoor temperature, resulting in energy waste and environmental pollution. In order to improve the living conditions of residents, rural area energy conservation has been put on the agenda. Based on the present situation and energy consumption analysis of the rural buildings in the hot summer and cold winter zone, this article puts forward several energy saving paths from government, construction technology and so on

  2. Behavior of a PCM at Varying Heating Rates: Experimental and Theoretical Study with an Aim at Temperature Moderation in Radionuclide Concrete Encasements

    NASA Astrophysics Data System (ADS)

    Medved', Igor; Trník, Anton

    2018-07-01

    Phase-change materials (PCMs) can store/release thermal energy within a small temperature range. This is of interest in various industrial applications, for example, in civil engineering (heating/cooling of buildings) or cold storage applications. Another application may be the moderation of temperature increases in concrete encasements of radionuclides during their decay. The phase-change behavior of a material is determined by its heat capacity and the peak it exhibits near a phase change. We analyze the behavior of such peaks for a selected PCM at heating rates varying between 0.1°C\\cdot min^{-1} and 1°C\\cdot min^{-1}, corresponding in real situations to different decay rates of radionuclides. We show that experimentally measured peaks can be plausibly described by an equilibrium theory that enables us to calculate the latent heat and phase-change temperature from experimental data.

  3. 1. Keeper's house, oil house, light tower and storage building, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Keeper's house, oil house, light tower and storage building, view northeast, south or southwest sides - Petit Manan Light Station, 2.5 miles south of Petit Manan Point, Milbridge, Washington County, ME

  4. 7. SOUTH ELEVATION OF BUILDING 262 (SOUTH SENTRY POST) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. SOUTH ELEVATION OF BUILDING 262 (SOUTH SENTRY POST) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  5. 2. SOUTHWEST CORNER OF BUILDING 259 (NORTH SENTRY POST) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. SOUTHWEST CORNER OF BUILDING 259 (NORTH SENTRY POST) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  6. 1. SOUTH ELEVATION OF BUILDING 259 (NORTH SENTRY POST) IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. SOUTH ELEVATION OF BUILDING 259 (NORTH SENTRY POST) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  7. Study of Charge Build Up in UCN Storage Cell

    NASA Astrophysics Data System (ADS)

    Broering, Mark; Abney, Josh; Swank, Christopher; Filippone, Bradley; Yao, Weijun; Korsch, Wolfgang

    2017-09-01

    The neutron EDM collaboration at the Spallation Neutron Source(ORNL) is using ultra-cold neutrons in superfluid helium to improve the nEDM limit by about two orders of magnitude. These neutrons will be stored in target cells located in a strong, stable electric field. Local radiation will generate charged particles which may build up on the target cell walls reducing field strength over time. The field changes need to be kept below 1%, making it necessary to study this cell charging behavior, determine its effect on the experiment and find ways to mitigate this. In order to study this cell charging effect, a compact test setup was designed. Using this scaled down model, charged particles are generated by a 137Cs source and the electric field is monitored via the electo-optic Kerr effect. Liquid nitrogen has a much stronger response to electric fields than helium, making it an ideal candidate for first tests. Cell charging effects have been observed in liquid nitrogen. These results along with the experimental technique and progress toward a superfluid helium measurement will also be presented. This research is supported by DOE Grants: DE-FG02-99ER41101, DE-AC05-00OR22725.

  8. KENNEDY SPACE CENTER, FLA. - Storage boxes and other containers of Columbia debris wait in the Columbia Debris Hangar for transfer to storage in the Vehicle Assembly Building. About 83,000 pieces were shipped to KSC during search and recovery efforts in East Texas.

    NASA Image and Video Library

    2003-09-02

    KENNEDY SPACE CENTER, FLA. - Storage boxes and other containers of Columbia debris wait in the Columbia Debris Hangar for transfer to storage in the Vehicle Assembly Building. About 83,000 pieces were shipped to KSC during search and recovery efforts in East Texas.

  9. Electricity demand and storage dispatch modeling for buildings and implications for the smartgrid

    NASA Astrophysics Data System (ADS)

    Zheng, Menglian; Meinrenken, Christoph

    2013-04-01

    As an enabler for demand response (DR), electricity storage in buildings has the potential to lower costs and carbon footprint of grid electricity while simultaneously mitigating grid strain and increasing its flexibility to integrate renewables (central or distributed). We present a stochastic model to simulate minute-by-minute electricity demand of buildings and analyze the resulting electricity costs under actual, currently available DR-enabling tariffs in New York State, namely a peak/offpeak tariff charging by consumed energy (monthly total kWh) and a time of use tariff charging by power demand (monthly peak kW). We then introduce a variety of electrical storage options (from flow batteries to flywheels) and determine how DR via temporary storage may increase the overall net present value (NPV) for consumers (comparing the reduced cost of electricity to capital and maintenance costs of the storage). We find that, under the total-energy tariff, only medium-term storage options such as batteries offer positive NPV, and only at the low end of storage costs (optimistic scenario). Under the peak-demand tariff, however, even short-term storage such as flywheels and superconducting magnetic energy offer positive NPV. Therefore, these offer significant economic incentive to enable DR without affecting the consumption habits of buildings' residents. We discuss implications for smartgrid communication and our future work on real-time price tariffs.

  10. Analysis for Eccentric Multi Canister Overpack (MCO) Drops at the Canister Storage Building (CSB) (CSB-S-0073)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    HOLLENBECK, R.G.

    The Spent Nuclear Fuel (SNF) Canister Storage Building (CSB) is the interim storage facility for the K-Basin SNF at the US. Department of Energy (DOE) Hanford Site. The SNF is packaged in multi-canister overpacks (MCOs). The MCOs are placed inside transport casks, then delivered to the service station inside the CSB. At the service station, the MCO handling machine (MHM) moves the MCO from the cask to a storage tube or one of two sample/weld stations. There are 220 standard storage tubes and six overpack storage tubes in a below grade reinforced concrete vault. Each storage tube can hold twomore » MCOs.« less

  11. Construction and operation of a support facilities (Building 729) for operation/testing of a prototype accelerator/storage ring (XLS) and machine shop for the National Synchrotron Light Source at Brookhaven National Laboratory, Upton, New York

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1992-06-01

    Proposed action is to construct at BNL a 5,600-ft[sup 2] support building, install and operate a prototypic 200 MeV accelerator and a prototypic 700 MeV storage ring within, and to construct and operate a 15 kV substation to power the building. The accelerator and storage ring would comprise the x-ray lithography source or XLS.

  12. Construction and operation of a support facilities (Building 729) for operation/testing of a prototype accelerator/storage ring (XLS) and machine shop for the National Synchrotron Light Source at Brookhaven National Laboratory, Upton, New York. Environmental assessment

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1992-06-01

    Proposed action is to construct at BNL a 5,600-ft{sup 2} support building, install and operate a prototypic 200 MeV accelerator and a prototypic 700 MeV storage ring within, and to construct and operate a 15 kV substation to power the building. The accelerator and storage ring would comprise the x-ray lithography source or XLS.

  13. Impacts of convection on high-temperature aquifer thermal energy storage

    NASA Astrophysics Data System (ADS)

    Beyer, Christof; Hintze, Meike; Bauer, Sebastian

    2016-04-01

    Seasonal subsurface heat storage is increasingly used in order to overcome the temporal disparities between heat production from renewable sources like solar thermal installations or from industrial surplus heat and the heat demand for building climatisation or hot water supply. In this context, high-temperature aquifer thermal energy storage (ATES) is a technology to efficiently store and retrieve large amounts of heat using groundwater wells in an aquifer to inject or withdraw hot or cold water. Depending on the local hydrogeology and temperature amplitudes during high-temperature ATES, density differences between the injected hot water and the ambient groundwater may induce significant convective flow components in the groundwater flow field. As a consequence, stored heat may accumulate at the top of the storage aquifer which reduces the heat recovery efficiency of the ATES system. Also, an accumulation of heat at the aquifer top will induce increased emissions of heat to overlying formations with potential impacts on groundwater quality outside of the storage. This work investigates the impacts of convective heat transport on the storage efficiency of a hypothetical high-temperature ATES system for seasonal heat storage as well as heat emissions to neighboring formations by numerical scenario simulations. The coupled groundwater flow and heat transport code OpenGeoSys is used to simulate a medium scale ATES system operating in a sandy aquifer of 20 m thickness with an average groundwater temperature of 10°C and confining aquicludes at top and bottom. Seasonal heat storage by a well doublet (i.e. one fully screened "hot" and "cold" well, respectively) is simulated over a period of 10 years with biannual injection / withdrawal cycles at pumping rates of 15 m³/h and for different scenarios of the temperature of the injected water (20, 35, 60 and 90 °C). Simulation results show, that for the simulated system significant convective heat transport sets in when injection temperatures exceed 35°C. Convection results in an accumulation of heat below the upper confining layer. The consequential increase of the heat plume contact area with this formation results in increased conductive heat transfer. Also, thermal gradients between the heat plume and the ambient groundwater increase with injection temperature, which increases heat conduction within the aquifer. Both effects reduce the thermal recovery of the ATES system. At the end of the 10th injection / withdrawal cycle the efficiency of thermal recovery thus reaches about 76 % for the 20°C scenario, 74% for 35°C, 71 % for 60°C and 66 % for the 90 °C scenario. Sensitivity analysis indicates that permeability in horizontal and vertical directions are controlling factors for the extent of convective heat displacement. Also, heat plume dimensions are influenced by permeability, and to a lesser extent by heat capacity and porosity of the aquifer. The planning of high-temperature ATES at a specific site hence requires a careful investigation of hydraulic and heat transport properties. Acknowledgments: This work is part of the ANGUS+ project (www.angusplus.de) and funded by the German Federal Ministry of Education and Research (BMBF) as part of the energy storage initiative "Energiespeicher".

  14. Role of Melatonin in Cell-Wall Disassembly and Chilling Tolerance in Cold-Stored Peach Fruit.

    PubMed

    Cao, Shifeng; Bian, Kun; Shi, Liyu; Chung, Hsiao-Hang; Chen, Wei; Yang, Zhenfeng

    2018-06-06

    Melatonin reportedly increases chilling tolerance in postharvest peach fruit during cold storage, but information on its effects on cell-wall disassembly in chilling-injured peaches is limited. In this study, we investigated the role of cell-wall depolymerization in chilling-tolerance induction in melatonin-treated peaches. Treatment with 100 μM melatonin alleviated chilling symptoms (mealiness) characterized by a decrease in fruit firmness and increase in juice extractability in treated peaches during storage. The loss of neutral sugars, such as arabinose and galactose, in both the 1,2-cyclohexylenedinitrilotetraacetic acid (CDTA)- and Na 2 CO 3 -soluble fractions was observed at 7 days in treated peaches, but the contents increased after 28 days of storage. Atomic-force-microscopy (AFM) analysis revealed that the polysaccharide widths in the CDTA- and Na 2 CO 3 -soluble fractions in the treated fruit were mainly distributed in a shorter range, as compared with those in the control fruit. In addition, the expression profiles of a series of cell-wall-related genes showed that melatonin treatment maintained the balance between transcripts of PpPME and PpPG, which accompany the up-regulation of several other genes involved in cell-wall disassembly. Taken together, our results suggested that the reduced mealiness by melatonin was probably associated with its positive regulation of numerous cell-wall-modifying enzymes and proteins; thus, the depolymerization of the cell-wall polysaccharides in the peaches treated with melatonin was maintained, and the treated fruit could soften gradually during cold storage.

  15. Cervarix, the GSK HPV-16/HPV-18 AS04-adjuvanted cervical cancer vaccine, demonstrates stability upon long-term storage and under simulated cold chain break conditions.

    PubMed

    Le Tallec, David; Doucet, Diane; Elouahabi, Abdelatif; Harvengt, Pol; Deschuyteneer, Michel; Deschamps, Marguerite

    2009-07-01

    Cervarix is a recombinant human papillomavirus (HPV)-16 and -18 L1 virus-like-particle (VLP) AS04-adjuvanted vaccine designed to protect against cervical intraepithelial neoplasia and cervical cancer caused by the HPV types 16 and 18. Assessment of the stability of the vaccine during long-term storage and after transient exposure to temperatures out of normal storage range is an integrated part of vaccine quality evaluation. This assessment was done with vaccine samples stored at 2-8 degrees C for up to 36 months, with or without simulated cold chain break (either one week at 37 degrees C, or two or four weeks at 25 degrees C). Among the stability-indicating parameters, antigenicity and immunogenicity were evaluated along with L1 antigen integrity and adsorption to aluminum. Differential scanning calorimetry (DSC) was used to investigate the structural stability of the VLPs before and after vaccine formulation and over time. Cervarix was stable at 2-8 degrees C for at least three years, and the occurrence of cold chain break had no impact, as shown by unchanged product characteristics during the full storage period. DSC analysis demonstrated that the structure of the HPV-16 and -18 L1 proteins and their corresponding VLPs was not affected throughout the manufacturing process. Moreover, the structure of aluminum-adsorbed HPV-16 and -18 L1 VLPs was robust over a 14-month test period. In conclusion, Cervarix was very stable upon long-term storage at 2-8 degrees C with or without transient exposure to higher temperatures (up to 37 degrees C). The observed robust structure of the L1 VLPs contributes to the excellent stability of Cervarix.

  16. Astrochemistry in TSR and CSR Ion Storage Rings

    NASA Astrophysics Data System (ADS)

    Novotny, Oldrich

    2017-04-01

    Dissociative recombination (DR) of molecular ions plays a key role in controlling the charge density and composition of the cold interstellar medium (ISM). Experimental data on DR are required in order to understand the chemical network in the ISM and related processes such as star formation from molecular clouds. Needed data include not only total reaction cross sections, but also the chemical composition and excitation states of the neutral products. Utilizing the TSR storage ring in Heidelberg, Germany, we have carried out DR measurements for astrophysically important molecular ions. We use a merged electron-ion beams technique combined with event-by-event fragment counting and fragment imaging. The count rate of detected neutral DR products yields the absolute DR rate coefficient. Imaging the distribution of fragment distances provides information on the kinetic energy released including the states of both the initial molecule and the final products. Additional kinetic energy sensitivity of the employed detector allows for identification of fragmentation channels by fragment-mass combination within each dissociation event. Such combined information is essential for studies on DR of polyatomic ions with multi-channel breakup. The recently commissioned Cryogenic Storage Ring (CSR) in Heidelberg, Germany, extends the experimental capabilities of TSR by operation at cryogenic temperatures down to 6 K. At these conditions residual gas densities down to 100 cm-3 can be reached resulting in beam storage times of several hours. Long storage in the cold environment allows the ions to relax down to their rotational ground state, thus mimicking well the conditions in the cold ISM. A variety of astrophysically relevant reactions will be investigated at these conditions, such as DR, electron impact excitation, ion-neutral collisions, etc. We report our TSR results on DR of HCl+ and D2Cl+. We also present first results from the CSR commissioning experiments.

  17. Growth medium alterations improve in vitro cold storage of pear germplasm.

    PubMed

    Kovalchuk, I; Zhumagulova, Z; Turdiev, T; Reed, B M

    2014-01-01

    Development of new fruit cultivars is dependent on genetic resource collections such as those at the Pomological Garden of the Institute of Horticulture and Viticulture near Almaty, Kazakhstan. The pear germplasm collection of the Pomological Garden contains 615 cultivars and three species. In vitro cold storage of the collection would provide additional security to the field collection. This study was designed to improve medium-term in vitro storage of pear germplasm. Shoots of seven pear cultivars (Pyrus communis L.) were stored in plastic five-section bags at 4 degree C and a 10-h photoperiod (7 μmol/m2/s). Treatments included medium with four carbohydrate sources (3% sucrose, 2% or 3% mannitol, or 2% sucrose + 2% mannitol) with 0.5 mg/l BAP and 0.1 mg/l IBA or without plant growth regulators (PGRs) and at three Murashige and Skoog (MS) nitrogen concentrations (100%, 50% or 25%). Pear shoots remained viable for 9 to 15 months without repropagation on the control MS medium with 3% sucrose without PGRs. There were significant impacts of cultivar and treatment on the duration of cold storage. Shoots of 'Mramornaya' remained viable (rating of ≥ 2) for 27 months with PGRs and 2% sucrose + 2% mannitol compared to 12 months for the PGR + 3% sucrose treatment. Talgarskaya Krasaviza stored for 18 months on 2% sucrose + 2% mannitol while all other treatments lasted only 6 to 9 months. Treatments with 0.5 or 1 mg/l abscisic acid (ABA) with 3% sucrose increased storage duration as did reducing the concentration of nitrogen in the medium to 25% without PGRs and with 3% sucrose.

  18. Applications and challenges for thermal energy storage

    NASA Astrophysics Data System (ADS)

    Kannberg, L. D.; Tomlinson, J. T.

    1991-04-01

    New thermal energy storage (TES) technologies are being developed and applied as society strives to relieve increasing energy and environmental stresses. Applications for these new technologies range from residential and district heating and cooling using waste and solar energy, to high-temperature energy storage for power production and industrial processes. In the last two decades there has been great interest and development of heat storage systems, primarily for residential and commercial buildings. While development has continued, the rate of advancement has slowed with current technology considered adequate for electrically charged heat storage furnaces. Use of chill storage for building diurnal cooling has received substantial development.

  19. Identifying Critical Factors in the Cost-Effectiveness of Solar and Battery Storage in Commercial Buildings

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McLaren, Joyce A.; Anderson, Katherine H.; Laws, Nicholas D.

    This analysis elucidates the emerging market for distributed solar paired with battery energy storage in commercial buildings across the United States. It provides insight into the near-term and future solar and solar-plus-storage market opportunities as well as the variables that impact the expected savings from installing behind-the-meter systems.

  20. Nonclassical storage and retrieval of a multiphoton pulse in cold Rydberg atoms

    NASA Astrophysics Data System (ADS)

    Tian, Xue-Dong; Liu, Yi-Mou; Bao, Qian-Qian; Wu, Jin-Hui; Artoni, M.; La Rocca, G. C.

    2018-04-01

    We investigate the storage and retrieval of a multiphoton probe field in cold Rydberg atoms with an effective method based on the superatom model. This probe field is found greatly attenuated in light intensity and two-photon correlation yet suffering little temporal broadening as a result of the partial dipole blockade of Rydberg excitation. In particular, the output field energy exhibits an intriguing saturation effect against the input field energy accompanied by an inhomogeneous nonclassical antibunching feature as a manifestation of the dynamic cooperative optical nonlinearity. Our numerical results are qualitatively consistent with those in a recent experiment and could be extended to pursue quantum information applications of nonclassical light fields.

  1. Studies on Superchilling of Fresh Skipjack

    NASA Astrophysics Data System (ADS)

    Matsuzaki, Yoh; Ogawa, Yutaka

    The freshness and meat colour of fresh skipjack can be retained only 2 or 3 days by ordinary cold storage. This paper deals with a new method, "suprchilling", which was developed by the authers, i. e., immediately after catch live skipjacks were immersed and killed instantly in NaCl brine at -10°C to -15°C for 30 to 40 minuites and thereafter in refrigerated sea water the center of the skipjack muscle was rapidly cooled to -0.5°C within 40 minuites. Form the results of apperance, organoleptic ratings, pH value, K value, metmyoglobin %, salt concentration and microscopic observation, "superchilling" could prolong the shelflife of skipjack 3 days longer than ordinary cold storage.

  2. A district survey of vaccine cold chain protection in general practitioners' surgeries.

    PubMed

    Finn, L; Crook, S

    1999-01-01

    Failure to ensure that vaccines are kept within a prescribed temperature range at all times can reduce their potency and cause primary vaccine failure. A postal survey of 103 general practices in a health district to assess vaccine handling and storage yielded 75 responses (73%). Poor practice was identified in receipt and storage of vaccines, temperature monitoring and control, management of vaccines during immunisation sessions, and disposal of partly used vaccines. The data suggest that the vaccine cold chain is not maintained with the degree of care necessary for safe practice. National guidelines need to be implemented conscientiously by all those involved with immunisation programmes if the effectiveness of vaccines is to be guaranteed.

  3. 35. FUEL HANDLING BUILDING, INTERIOR LOOKING SOUTHEAST SHOWING TRANSFER CANAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    35. FUEL HANDLING BUILDING, INTERIOR LOOKING SOUTHEAST SHOWING TRANSFER CANAL AREA, DEEP STORAGE AREA, FUEL STORAGE PIT (LOCATION BB) - Shippingport Atomic Power Station, On Ohio River, 25 miles Northwest of Pittsburgh, Shippingport, Beaver County, PA

  4. 13. Interior view of food storage area looking towards hallway; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Interior view of food storage area looking towards hallway; southeast corner of building on lower floor; view to north. - Ellsworth Air Force Base, Mess & Administration Building, 1561 Ellsworth Street, Blackhawk, Meade County, SD

  5. VIEW ALONG SUPPORT ROAD, LOOKING TOWARD ELEVATED WATER STORAGE TANK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW ALONG SUPPORT ROAD, LOOKING TOWARD ELEVATED WATER STORAGE TANK (BUILDING 2824), WITH EDUCATION CENTER (BUILDING 2670) AT LEFT BACKGROUND. VIEW TO NORTHEAST - Plattsburgh Air Force Base, U.S. Route 9, Plattsburgh, Clinton County, NY

  6. EAST ELEVATION OF HIGH BAY ADDITION OF FUEL STORAGE BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    EAST ELEVATION OF HIGH BAY ADDITION OF FUEL STORAGE BUILDING (CPP-603). INL DRAWING NUMBER 200-0603-00-706-051286. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  7. 14. VEHICLE STORAGE BUILDING NORTHWEST SIDE AND NORTHEAST REAR. VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. VEHICLE STORAGE BUILDING NORTHWEST SIDE AND NORTHEAST REAR. VIEW TO SOUTHEAST. - Minuteman III ICBM Launch Control Facility November-1, 1.5 miles North of New Raymer & State Highway 14, New Raymer, Weld County, CO

  8. 13. VEHICLE STORAGE BUILDING SOUTHWEST FRONT AND SOUTHEAST SIDE. VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VEHICLE STORAGE BUILDING SOUTHWEST FRONT AND SOUTHEAST SIDE. VIEW TO NORTHWEST. - Minuteman III ICBM Launch Control Facility November-1, 1.5 miles North of New Raymer & State Highway 14, New Raymer, Weld County, CO

  9. 20. VIEW SOUTHEAST OF UPPER LEVEL (LUMBER STORAGE) IN EAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. VIEW SOUTHEAST OF UPPER LEVEL (LUMBER STORAGE) IN EAST BUILDING SHOWING BUILDING FRAMEWORK, IRON HANGING RODS, AND CEDAR PLANKS BEING SEASONED FOR BOAT CONSTRUCTION. - Lowell's Boat Shop, 459 Main Street, Amesbury, Essex County, MA

  10. A brief history of 20th century dam construction and a look into the future

    NASA Astrophysics Data System (ADS)

    van de Giesen, Nick

    2010-05-01

    In this presentation, an overview is given of global dam building activities in the 20th century. Political, economical and hydrological factors shaped the building of large dams. The development of the relations between these three factors and dam building over time is examined. One can argue whether or not history is simply "one damn thing after another" but the second half of the 20th century suggests that history is at least reflected by the construction of one dam after another. The financial crisis of the 1930's started the first construction wave of large hydropower dams in the United States. This wave continued into the Second World War. During the Cold War, the weapon race between the USA and USSR was accompanied by a parallel neck-and-neck race in dam construction. By the 1970's, dam construction in the USA tapered off, while that in the USSR continued until its political disintegration. In China, we see two spurts in dam development, the first one coinciding with the disastrous Great Leap Forward and the second with the liberalization of the Chinese economy after the fall of the Berlin Wall. Economic and political events thus shaped to an important extent decisions surrounding the construction of large dams. Clearly, there are some hydrological prerequisites for the construction of dams. The six largest dam building nations are USSR, Canada, USA, China, Brazil, and India, all large countries with ample water resources and mountain ranges. Australia has relatively little reservoir storage for the simple fact that most of this country is flat and dry. A few countries have relatively large amounts of reservoir storage. Especially Uganda (Owens Falls), Ghana (Akosombo), and Zimbabwe (Kariba) are examples of small countries where gorges in major rivers were "natural" places for large dams and reservoirs to be built early on. It seems that, deserts aside, the average potential storage capacity lies for most continents around 10 cm or about 50% of the total yearly continental runoff. Some of the least developed countries, such as Papua New Guinea, Congo DR, and Myanmar, still have large hydropower development potential. In most countries, however, dam construction seems to have reached its peak. For the presentation, use is made of GapMinder software (www.gapminder.org), which provides direct insight in the dynamic and multi-dimensonial aspects of 20th century dam construction.

  11. Effect of sulfur dioxide fumigation on survival of foodborne pathogens on table grapes under standard storage temperature

    USDA-ARS?s Scientific Manuscript database

    We examined the persistence of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella enterica Thompson inoculated on freshly-harvested table grapes under standard cold storage with initial and weekly sulfur dioxide (SO2) fumigation. L. monocytogenes and S. enterica Thompson were much more...

  12. Antioxidant activity and protein-polyphenol interactions in a pomegranate (Punica granatum L.) yogurt.

    PubMed

    Trigueros, Lorena; Wojdyło, Aneta; Sendra, Esther

    2014-07-09

    Pomegranate juice (PGJ) is rich in phenolics which are potent antioxidants but also prone to interact with proteins. A yogurt rich in PGJ (40%) made from arils was elaborated (PGY) to determine the antioxidant activity and to estimate the phenolics-proteins interaction during 28 days of cold storage. Juice, yogurts, and protein-free permeates were analyzed for phenolic composition. Yogurt fermentation modified the anthocyanin profile of the initial PGJ, especially the content in cyanidin-3-O-glucoside. During storage, individual anthocyanin content in PGY decreased but it did not modify yogurt color. The analysis of permeates revealed that the degree of phenol-protein interaction depends on the type of phenolic, ellagic acid and dephinidin-3,5-O-diglucoside being the least bound phenolic compounds. The presence of PGJ in yogurt enhanced radical scavenging performance, whereas all the observed ferric reducing power ability of PGY was strictly due to the PGJ present. The 84.73% of total anthocyanins remained bound to proteins at the first day of storage and 90.06% after 28 days of cold storage, revealing the high affinity of anthocyanins for milk proteins.

  13. Optimization and Performance Study of Select Heating Ventilation and Air Conditioning Technologies for Commercial Buildings

    NASA Astrophysics Data System (ADS)

    Kamal, Rajeev

    Buildings contribute a significant part to the electricity demand profile and peak demand for the electrical utilities. The addition of renewable energy generation adds additional variability and uncertainty to the power system. Demand side management in the buildings can help improve the demand profile for the utilities by shifting some of the demand from peak to off-peak times. Heating, ventilation and air-conditioning contribute around 45% to the overall demand of a building. This research studies two strategies for reducing the peak as well as shifting some demand from peak to off-peak periods in commercial buildings: 1. Use of gas heat pumps in place of electric heat pumps, and 2. Shifting demand for air conditioning from peak to off-peak by thermal energy storage in chilled water and ice. The first part of this study evaluates the field performance of gas engine-driven heat pumps (GEHP) tested in a commercial building in Florida. Four GEHP units of 8 Tons of Refrigeration (TR) capacity each providing air-conditioning to seven thermal zones in a commercial building, were instrumented for measuring their performance. The operation of these GEHPs was recorded for ten months, analyzed and compared with prior results reported in the literature. The instantaneous COPunit of these systems varied from 0.1 to 1.4 during typical summer week operation. The COP was low because the gas engines for the heat pumps were being used for loads that were much lower than design capacity which resulted in much lower efficiencies than expected. The performance of equivalent electric heat pump was simulated from a building energy model developed to mimic the measured building loads. An economic comparison of GEHPs and conventional electrical heat pumps was done based on the measured and simulated results. The average performance of the GEHP units was estimated to lie between those of EER-9.2 and EER-11.8 systems. The performance of GEHP systems suffers due to lower efficiency at part load operation. The study highlighted the need for optimum system sizing for GEHP/HVAC systems to meet the building load to obtain better performance in buildings. The second part of this study focusses on using chilled water or ice as thermal energy storage for shifting the air conditioning load from peak to off-peak in a commercial building. Thermal energy storage can play a very important role in providing demand-side management for diversifying the utility demand from buildings. Model of a large commercial office building is developed with thermal storage for cooling for peak power shifting. Three variations of the model were developed and analyzed for their performance with 1) ice storage, 2) chilled water storage with mixed storage tank and 3) chilled water storage with stratified tank, using EnergyPlus 8.5 software developed by the US Department of Energy. Operation strategy with tactical control to incorporate peak power schedule was developed using energy management system (EMS). The modeled HVAC system was optimized for minimum cost with the optimal storage capacity and chiller size using JEPlus. Based on the simulation, an optimal storage capacity of 40-45 GJ was estimated for the large office building model along with 40% smaller chiller capacity resulting in higher chiller part-load performance. Additionally, the auxiliary system like pump and condenser were also optimized to smaller capacities and thus resulting in less power demand during operation. The overall annual saving potential was found in the range of 7-10% for cooling electricity use resulting in 10-17% reduction in costs to the consumer. A possible annual peak shifting of 25-78% was found from the simulation results after comparing with the reference models. Adopting TES in commercial buildings and achieving 25% peak shifting could result in a reduction in peak summer demand of 1398 MW in Tampa.

  14. KENNEDY SPACE CENTER, FLA. - Storage boxes filled with Columbia debris (left) await transfer to storage in the Vehicle Assembly Building. Empty boxes at right wait to be filled with more of the approximately 83,000 pieces shipped to KSC during search and recovery efforts in East Texas.

    NASA Image and Video Library

    2003-09-02

    KENNEDY SPACE CENTER, FLA. - Storage boxes filled with Columbia debris (left) await transfer to storage in the Vehicle Assembly Building. Empty boxes at right wait to be filled with more of the approximately 83,000 pieces shipped to KSC during search and recovery efforts in East Texas.

  15. Referenced-site environmental document for a Monitored Retrievable Storage facility: backup waste management option for handling 1800 MTU per year

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Silviera, D.J.; Aaberg, R.L.; Cushing, C.E.

    This environmental document includes a discussion of the purpose of a monitored retrievable storage facility, a description of two facility design concepts (sealed storage cask and field drywell), a description of three reference sites (arid, warm-wet, and cold-wet), and a discussion and comparison of the impacts associated with each of the six site/concept combinations. This analysis is based on a 15,000-MTU storage capacity and a throughput rate of up to 1800 MTU per year.

  16. Predictive Optimal Control of Active and Passive Building Thermal Storage Inventory

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gregor P. Henze; Moncef Krarti

    2005-09-30

    Cooling of commercial buildings contributes significantly to the peak demand placed on an electrical utility grid. Time-of-use electricity rates encourage shifting of electrical loads to off-peak periods at night and weekends. Buildings can respond to these pricing signals by shifting cooling-related thermal loads either by precooling the building's massive structure or the use of active thermal energy storage systems such as ice storage. While these two thermal batteries have been engaged separately in the past, this project investigated the merits of harnessing both storage media concurrently in the context of predictive optimal control. To pursue the analysis, modeling, and simulationmore » research of Phase 1, two separate simulation environments were developed. Based on the new dynamic building simulation program EnergyPlus, a utility rate module, two thermal energy storage models were added. Also, a sequential optimization approach to the cost minimization problem using direct search, gradient-based, and dynamic programming methods was incorporated. The objective function was the total utility bill including the cost of reheat and a time-of-use electricity rate either with or without demand charges. An alternative simulation environment based on TRNSYS and Matlab was developed to allow for comparison and cross-validation with EnergyPlus. The initial evaluation of the theoretical potential of the combined optimal control assumed perfect weather prediction and match between the building model and the actual building counterpart. The analysis showed that the combined utilization leads to cost savings that is significantly greater than either storage but less than the sum of the individual savings. The findings reveal that the cooling-related on-peak electrical demand of commercial buildings can be considerably reduced. A subsequent analysis of the impact of forecasting uncertainty in the required short-term weather forecasts determined that it takes only very simple short-term prediction models to realize almost all of the theoretical potential of this control strategy. Further work evaluated the impact of modeling accuracy on the model-based closed-loop predictive optimal controller to minimize utility cost. The following guidelines have been derived: For an internal heat gain dominated commercial building, reasonable geometry simplifications are acceptable without a loss of cost savings potential. In fact, zoning simplification may improve optimizer performance and save computation time. The mass of the internal structure did not show a strong effect on the optimization. Building construction characteristics were found to impact building passive thermal storage capacity. It is thus advisable to make sure the construction material is well modeled. Zone temperature setpoint profiles and TES performance are strongly affected by mismatches in internal heat gains, especially when they are underestimated. Since they are a key factor in determining the building cooling load, efforts should be made to keep the internal gain mismatch as small as possible. Efficiencies of the building energy systems affect both zone temperature setpoints and active TES operation because of the coupling of the base chiller for building precooling and the icemaking TES chiller. Relative efficiencies of the base and TES chillers will determine the balance of operation of the two chillers. The impact of mismatch in this category may be significant. Next, a parametric analysis was conducted to assess the effects of building mass, utility rate, building location and season, thermal comfort, central plant capacities, and an economizer on the cost saving performance of optimal control for active and passive building thermal storage inventory. The key findings are: (1) Heavy-mass buildings, strong-incentive time-of-use electrical utility rates, and large on-peak cooling loads will likely lead to attractive savings resulting from optimal combined thermal storage control. (2) By using economizer to take advantage of the cool fresh air during the night, the building electrical cost can be reduced by using less mechanical cooling. (3) Larger base chiller and active thermal storage capacities have the potential of shifting more cooling loads to off-peak hours and thus higher savings can be achieved. (4) Optimal combined thermal storage control with a thermal comfort penalty included in the objective function can improve the thermal comfort levels of building occupants when compared to the non-optimized base case. Lab testing conducted in the Larson HVAC Laboratory during Phase 2 showed that the EnergyPlus-based simulation was a surprisingly accurate prediction of the experiment. Therefore, actual savings of building energy costs can be expected by applying optimal controls from simulation results.« less

  17. 5. View of interior, EPA Farm Lab Building 1506 slaughter ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View of interior, EPA Farm Lab Building 15-06 slaughter addition (featuring cold slaughter area), facing north-northeast - Nevada Test Site, Environmental Protection Agency Farm, Laboratory Building, Area 15, Yucca Flat, 10-2 Road near Circle Road, Mercury, Nye County, NV

  18. Empirical Profiling of Cold Hydrogen Plumes Formed from Venting Of LH2 Storage Vessels: Preprint

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Buttner, William J; Rivkin, Carl H; Schmidt, Kara

    Liquid hydrogen (LH2) storage is a viable approach to assuring sufficient hydrogen capacity at commercial fuelling stations. Presently, LH2 is produced at remote facilities and then transported to the end-use site by road vehicles (i.e., LH2 tanker trucks). Venting of hydrogen to depressurize the transport storage tank is a routine part of the LH2 delivery process. The behaviour of cold hydrogen plumes has not been well-characterized because empirical field data is essentially non-existent. The NFPA 2 Hydrogen Storage Safety Task Group, which consists of hydrogen producers, safety experts, and CFD modellers, has identified the lack of understanding of hydrogen dispersionmore » during LH2 venting of storage vessel as a critical gap for establishing safety distances at LH2 facilities, especially commercial hydrogen fuelling stations. To address this need, the NREL sensor laboratory, in collaboration with the NFPA 2 Safety Task Group developed the Cold Hydrogen Plume Analyzer to empirically characterize the hydrogen plume formed during LH2 storage tank venting. A prototype Analyzer was developed and field-deployed at an actual LH2 venting operation with critical findings that included: - H2 being detected as much as 2 m lower than the release point, which is not predicted by existing models - A small and inconsistent correlation between oxygen depletion and the hydrogen concentration - A negligible to non-existent correlation between in-situ temperature and the hydrogen concentration The Analyzer is currently being upgraded for enhanced metrological capabilities including improved real-time spatial and temporal profiling of the plume and tracking of prevailing weather conditions. Additional deployments are planned to monitor plume behaviour under different wind, humidity, and temperatures. This data will be shared with the NFPA 2 Safety Task Group and ultimately will be used support theoretical models and code requirements prescribed in NFPA 2.« less

  19. General regression neural network model for behavior of Salmonella on chicken meat during cold storage.

    PubMed

    Oscar, Thomas P

    2014-05-01

    A study was undertaken to investigate and model behavior of Salmonella on chicken meat during cold storage at constant temperatures. Chicken meat (white, dark, or skin) portions (0.75 cm(3)) were inoculated with a single strain of Salmonella Typhimurium DT104 (2.8 log) followed by storage for 0 to 8 d at -8, 0, 8, 12, 14, or 16 °C for model development and at -4, 4, 10, or 14 °C for model validation. A general regression neural network model was developed with commercial software. Performance of the model was considered acceptable when the proportion of residuals (observed--predicted) in an acceptable prediction zone (pAPZ) from -1 log (fail-safe) to 0.5 logs (fail-dangerous) was ≥ 0.7. Growth of Salmonella Typhimurium DT104 on chicken meat was observed at 12, 14, and 16 °C and was highest on dark meat, intermediate on skin, and lowest on white meat. At lower temperatures (-8 to 10 °C) Salmonella Typhimurium DT104 remained at initial levels throughout 8 d of storage except at 4 °C where there was a small (0.4 log) but significant decline. The model had acceptable performance (pAPZ = 0.929) for dependent data (n = 482) and acceptable performance (pAPZ = 0.923) for independent data (n = 235). Results indicated that it is important to include type of meat as an independent variable in the model and that the model provided valid predictions of the behavior of Salmonella Typhimurium DT104 on chicken skin, white, and dark meat during storage for 0 to 8 d at constant temperatures from -8 to 16 °C. A model for predicting behavior of Salmonella on chicken meat during cold storage was developed and validated. The model will help the chicken industry to better predict and manage this risk to public health. Journal of Food Science © 2014 Institute of Food Technologists® No claim to original US government works.

  20. Evaluation of different multidimensional LC-MS/MS pipelines for iTRAQ-based proteomic analysis of potato tubers in response to cold storage

    USDA-ARS?s Scientific Manuscript database

    Cold-induced sweetening in potato tubers is a costly problem for food industry. To systematically identify the proteins associated with this process, we employed a comparative proteomics approach using isobaric, stable isotope coded labels to compare the proteomes of potato tubers after 0 and 5 mont...

  1. Inactivation of Escherichia coli 0157:H7 and aerobic microorganisms in Romaine lettuce packaged in a commercial polyethylene terephthalate container using atmospheric cold plasma

    USDA-ARS?s Scientific Manuscript database

    The effects of dielectric barrier discharge atmospheric cold plasma (DACP) treatment on the inactivation of Escherichia coli O157:H7 and aerobic microorganisms in Romaine lettuce packaged in a conventional commercial plastic container were evaluated during storage at 4 degrees C for 7 days. Effects ...

  2. 90. VIEW NORTHEAST OF BUILDING 98 OIL HOUSE AND STORAGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    90. VIEW NORTHEAST OF BUILDING 98 OIL HOUSE AND STORAGE TANK; OIL FOR POWER GENERATION WAS UNLOADED FROM TANK CARS AND STORED AT THIS FACILITY - Scovill Brass Works, 59 Mill Street, Waterbury, New Haven County, CT

  3. 9. DETAIL OF GUNPORT ON SOUTH ELEVATION OF BUILDING 262 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. DETAIL OF GUNPORT ON SOUTH ELEVATION OF BUILDING 262 (SOUTH SENTRY POST) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  4. Design optimization of cold-formed steel portal frames taking into account the effect of building topology

    NASA Astrophysics Data System (ADS)

    Phan, Duoc T.; Lim, James B. P.; Sha, Wei; Siew, Calvin Y. M.; Tanyimboh, Tiku T.; Issa, Honar K.; Mohammad, Fouad A.

    2013-04-01

    Cold-formed steel portal frames are a popular form of construction for low-rise commercial, light industrial and agricultural buildings with spans of up to 20 m. In this article, a real-coded genetic algorithm is described that is used to minimize the cost of the main frame of such buildings. The key decision variables considered in this proposed algorithm consist of both the spacing and pitch of the frame as continuous variables, as well as the discrete section sizes. A routine taking the structural analysis and frame design for cold-formed steel sections is embedded into a genetic algorithm. The results show that the real-coded genetic algorithm handles effectively the mixture of design variables, with high robustness and consistency in achieving the optimum solution. All wind load combinations according to Australian code are considered in this research. Results for frames with knee braces are also included, for which the optimization achieved even larger savings in cost.

  5. Mitigation of Corrosion in 5 Series Al-Mg Alloys in Marine Environments: Grain Boundary Engineering and Cold Spray Coating Approaches

    DTIC Science & Technology

    2014-03-26

    powders for cold spray are nominally ductile materials such as Cu and Al or Al alloys with particles in the 5-45|am size range. It is for...wavelength) as the x-ray source. Since cold spray is a solid state deposition process , the composition and microstructure of the feedstock powder ...surface of the recently deposited coating build up and a thick coating with theoretical bulk properties can be achieved [27]. The cold

  6. Salt and smoke simultaneously affect chemical and sensory quality of cold-smoked salmon during 5 degrees C storage predicted using factorial design.

    PubMed

    Leroi, F; Joffraud, J J

    2000-09-01

    Simultaneous effect of salt and smoke on chemical indices of cold-smoked salmon and on its shelf life, estimated by sensory analysis, was investigated during vacuum-packed storage at 5 degrees C. Salting salmon immediately decreased the pH in the flesh, probably due to the increase of the ionic force, then pH remained constant during storage. Total volatile base nitrogen and trimethylamine productions were mainly inhibited by the salt concentration in the flesh, whereas phenol had no effect. A highly synergistic effect between the two factors was observed on the shelf life response. When a high level of salt (5% wt/wt) or phenol (1 mg 100 g(-1)) was added separately, shelf life did not exceed 1 week, whereas it could reach more than 10 weeks when salt and smoke were added simultaneously. Different combinations were examined for shelf life characteristics of the product. For instance, 2 and 3% (wt/wt) of salt with, respectively, 0.80 and 0.45 mg 100 g(-1) of phenol were sufficient for a 4-week shelf life, satisfying most of French cold-smoked salmon producers and consumers. Correlation between microbiological responses measured in a previous study and chemical and sensory data were also established.

  7. PBF Reactor Building (PER620). Plot plan shows layout, including auxiliary ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PBF Reactor Building (PER-620). Plot plan shows layout, including auxiliary buildings: Emergency Generator (621), Hose House (622), Cooling Tower Auxiliary (624), Maintenance and Storage Warehouse (625), Gas Cylinder Storage (627), Hose House (628), Cooling Tower (720), Substation (719), and other features. Road connections between PBF Reactor, its control building, and SPERT-I site. Note cable trenches along road to control building. Date: July 1965. Ebasco Services, PER-U-101. INEEL index no. 761-0100-00-205-123005 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

  8. Peak reduction for commercial buildings using energy storage

    NASA Astrophysics Data System (ADS)

    Chua, K. H.; Lim, Y. S.; Morris, S.

    2017-11-01

    Battery-based energy storage has emerged as a cost-effective solution for peak reduction due to the decrement of battery’s price. In this study, a battery-based energy storage system is developed and implemented to achieve an optimal peak reduction for commercial customers with the limited energy capacity of the energy storage. The energy storage system is formed by three bi-directional power converter rated at 5 kVA and a battery bank with capacity of 64 kWh. Three control algorithms, namely fixed-threshold, adaptive-threshold, and fuzzy-based control algorithms have been developed and implemented into the energy storage system in a campus building. The control algorithms are evaluated and compared under different load conditions. The overall experimental results show that the fuzzy-based controller is the most effective algorithm among the three controllers in peak reduction. The fuzzy-based control algorithm is capable of incorporating a priori qualitative knowledge and expertise about the load characteristic of the buildings as well as the useable energy without over-discharging the batteries.

  9. Influence of Cold Exposure on Ventilation, Respiratory Heat Loss, and Pulmonary Deposition/Clearance

    DTIC Science & Technology

    1990-06-25

    mechanism(s) by which cold stimulates a change in breathing pattern were investigated in male Holstein calves between the ages of 1 and 3 months. The...with a decrease of 6 breaths/min over a 10C fall in temperature. McLean et al. (69) observed a similar relationship in 3 to 12-year-old cows with a...Whitmore, B.A. Young and R. Weingardt. Body heat storage, metabolism and respiration of cows abruptly exposed and acclimatized to cold and 18°C

  10. Monitoring adherence to cold chain storage of vaccines in 2014 in the Małopolska province

    PubMed

    Wiercińska, Ewa; Orzeł-Nowak, Anita; Mrozowska, Barbara; Foremny, Jarosław

    2017-01-01

    The principles of „cold chain” have been developed for the safe transport and storage of vaccines because of their sensitivity to temperature and are guidelines for use in all countries. Available information on adherence to the principles of the “cold chain” by healthcare providers in Poland is insufficient. The aim of the study was to assess compliance with these rules in storing vaccines by vaccination centers implementing an immunization program in 2014 in the Malopolska province The study was conducted in 111 vaccination centers in 19 districts of Malopolska, which represents 12% of all vaccination centers in the Malopolska province in 2014. The selection of vaccination centers for the study was not random. During a routine inspection of vaccination centers, an employee of the District Sanitary-Epidemiological Station conducted a study using a specially-prepared questionnaire. Inspections in vaccination centers which qualified for the study were conducted from March to September 2015. Among the analyzed vaccinations centers 90% had a refrigerator or refrigerator-freezer, and 10% a pharmaceutical refrigerator. 3.6% of refrigerators were produced before 2000. A 24-hour recording of the temperature was conducted in 67.6% vaccination centers. 40.5% of vaccination centers had a 24-hour system of recording and notification when temperature parameters had been exceeded in refrigeration devices. 22.5% of vaccination centers had emergency power supply. 13.5% of vaccination centers a power failure occurred in refrigerators, including 40% of the vaccination centers which had a text message notification system.. Total cost of destroyed vaccines amounted to over 20 thousand zl. Correct storage conditions are needed for vaccines to maintain their full effectiveness, which is why it is necessary to intensify efforts to ensure compliance with the principles of the cold chain in the storage of vaccines by healthcare providers.

  11. The COLD-SAT Experiment for Cryogenic Fluid Management Technology

    NASA Technical Reports Server (NTRS)

    Schuster, J. R.; Wachter, J. P.; Vento, D. M.

    1990-01-01

    Future national space transportation missions will depend on the use of cryogenic fluid management technology development needs for these missions. In-space testing will be conducted in order to show low gravity cryogenic fluid management concepts and to acquire a technical data base. Liquid H2 is the preferred test fluid due to its propellant use. The design of COLD-SAT (Cryogenic On-orbit Liquid Depot Storage, Acquisition, and Transfer Satellite), an Expendable Launch Vehicle (ELV) launched orbital spacecraft that will perform subcritical liquid H2 storage and transfer experiments under low gravity conditions is studied. An Atlas launch vehicle will place COLD-SAT into a circular orbit, and the 3-axis controlled spacecraft bus will provide electric power, experiment control, and data management, attitude control, and propulsive accelerations for the experiments. Low levels of acceleration will provide data on the effects that low gravity might have on the heat and mass transfer processes used. The experiment module will contain 3 liquid H2 tanks; fluid transfer, pressurization and venting equipment; and instrumentation.

  12. Post-translational regulation of acid invertase activity by vacuolar invertase inhibitor affects resistance to cold-induced sweetening of potato tubers.

    PubMed

    McKenzie, Marian J; Chen, Ronan K Y; Harris, John C; Ashworth, Matthew J; Brummell, David A

    2013-01-01

    Cold-induced sweetening (CIS) is a serious post-harvest problem for potato tubers, which need to be stored cold to prevent sprouting and pathogenesis in order to maintain supply throughout the year. During storage at cold temperatures (below 10 °C), many cultivars accumulate free reducing sugars derived from a breakdown of starch to sucrose that is ultimately cleaved by acid invertase to produce glucose and fructose. When affected tubers are processed by frying or roasting, these reducing sugars react with free asparagine by the Maillard reaction, resulting in unacceptably dark-coloured and bitter-tasting product and generating the probable carcinogen acrylamide as a by-product. We have previously identified a vacuolar invertase inhibitor (INH2) whose expression correlates both with low acid invertase activity and with resistance to CIS. Here we show that, during cold storage, overexpression of the INH2 vacuolar invertase inhibitor gene in CIS-susceptible potato tubers reduced acid invertase activity, the accumulation of reducing sugars and the generation of acrylamide in subsequent fry tests. Conversely, suppression of vacuolar invertase inhibitor expression in a CIS-resistant line increased susceptibility to CIS. The results show that post-translational regulation of acid invertase by the vacuolar invertase inhibitor is an important component of resistance to CIS. © 2012 Blackwell Publishing Ltd.

  13. Thermal performance of phase change wallboard for residential cooling application

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Feustel, H.E.; Stetiu, C.

    1997-04-01

    Cooling of residential California buildings contributes significantly to electrical consumption and peak power demand mainly due to very poor load factors in milder climates. Thermal mass can be utilized to reduce the peak-power demand, downsize the cooling systems, and/or switch to low-energy cooling sources. Large thermal storage devices have been used in the past to overcome the shortcomings of alternative cooling sources, or to avoid high demand charges. The manufacturing of phase change material (PCM) implemented in gypsum board, plaster or other wall-covering material, would permit the thermal storage to become part of the building structure. PCMs have two importantmore » advantages as storage media: they can offer an order-of-magnitude increase in thermal storage capacity, and their discharge is almost isothermal. This allows the storage of high amounts of energy without significantly changing the temperature of the room envelope. As heat storage takes place inside the building, where the loads occur, rather than externally, additional transport energy is not required. RADCOOL, a thermal building simulation program based on the finite difference approach, was used to numerically evaluate the latent storage performance of treated wallboard. Extended storage capacity obtained by using double PCM-wallboard is able to keep the room temperatures close to the upper comfort limits without using mechanical cooling. Simulation results for a living room with high internal loads and weather data for Sunnyvale, California, show significant reduction of room air temperature when heat can be stored in PCM-treated wallboards.« less

  14. Oblique view of front (east) and south sides of ammunition ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of front (east) and south sides of ammunition storage buildings 4403 and 4404, view towards the northwest without scale - Fort McClellan Ammunition Storage Area, Building No. 4403, Second Avenue (Magazine Road), Anniston, Calhoun County, AL

  15. Interior planar view of doors to railroad platform at rear ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior planar view of doors to railroad platform at rear of ammunition storage building 4403, view towards the west westside with scale - Fort McClellan Ammunition Storage Area, Building No. 4403, Second Avenue (Magazine Road), Anniston, Calhoun County, AL

  16. CONSTRUCTION VIEW FUEL STORAGE BUILDING (CPP603) LOOKING EAST SHOWING ASBESTOS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CONSTRUCTION VIEW FUEL STORAGE BUILDING (CPP-603) LOOKING EAST SHOWING ASBESTOS SIDING. INL PHOTO NUMBER NRTS-51-1543. Unknown Photographer, 2/28/1951 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  17. CONSTRUCTION PROGRESS PHOTO SHOWING FUEL STORAGE BUILDING (CPP603) LOOKING NORTHWEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CONSTRUCTION PROGRESS PHOTO SHOWING FUEL STORAGE BUILDING (CPP-603) LOOKING NORTHWEST. INL PHOTO NUMBER NRTS-50-895. Unknown Photographer, 10/30/1950 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  18. 4. Storage building, outhouse, oil house, keeper's house and light ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Storage building, outhouse, oil house, keeper's house and light tower, view southwest, northeast sides (southeast and northeast sides of keeper's house) - Petit Manan Light Station, 2.5 miles south of Petit Manan Point, Milbridge, Washington County, ME

  19. Evaluation of in vitro storage characteristics of cold stored platelet concentrates with N acetylcysteine (NAC).

    PubMed

    Handigund, Mallikarjun; Bae, Tae Won; Lee, Jaehyeon; Cho, Yong Gon

    2016-02-01

    Platelets play a vital role in hemostasis and thrombosis, and their demand and usage has multiplied many folds over the years. However, due to the short life span and storage constraints on platelets, it is allowed to store them for up to 7 days at room temperature (RT); thus, there is a need for an alternative storage strategy for extension of shelf life. Current investigation involves the addition of 50 mM N acetylcysteine (NAC) in refrigerated concentrates. Investigation results revealed that addition of NAC to refrigerated concentrates prevented platelet activation and reduced the sialidase activity upon rewarming as well as on prolonged storage. Refrigerated concentrates with 50 mM NAC expressed a 23.91 ± 6.23% of CD62P (P-Selectin) and 22.33 ± 3.42% of phosphotidylserine (PS), whereas RT-stored platelets showed a 46.87 ± 5.23% of CD62P and 25.9 ± 6.48% of phosphotidylserine (PS) after 5 days of storage. Further, key metabolic parameters such as glucose and lactate accumulation indicated reduced metabolic activity. Taken together, investigation and observations indicate that addition of NAC potentially protects refrigerated concentrates by preventing platelet activation, stabilizing sialidase activity, and further reducing the metabolic activity. Hence, we believe that NAC can be a good candidate for an additive solution to retain platelet characteristics during cold storage and may pave the way for extension of storage shelf life. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Air temperature optimisation for humidity-controlled cold storage of the predatory mites Neoseiulus californicus and Phytoseiulus persimilis (Acari: Phytoseiidae).

    PubMed

    Ghazy, Noureldin Abuelfadl; Suzuki, Takeshi; Amano, Hiroshi; Ohyama, Katsumi

    2014-03-01

    Humidity-controlled cold storage, in which the water vapour pressure is saturated, can prolong the survival of the predatory mites Neoseiulus californicus (McGregor) and Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae). However, information on the optimum air temperature for long-term storage by this method is limited. The authors evaluated the survival of mated adult females of N. californicus and P. persimilis at 5.0, 7.5, 10.0 and 12.5 °C under saturated water vapour condition (vapour pressure deficit 0.0 kPa). N. californicus showed a longer survival time than P. persimilis at all the air temperatures. The longest mean survival time of N. californicus was 11 weeks at 7.5 °C, whereas that of P. persimilis was 8 weeks at 5.0 °C. After storage at 7.5 °C for 8 weeks, no negative effect on post-storage oviposition was observed in N. californicus, whereas the oviposition of P. persimilis stored at 5.0 °C for 8 weeks was significantly reduced. The interspecific variation in the response of these predators to low air temperature might be attributed to their natural habitat and energy requirements. These results may be useful for the long-term storage of these predators, which is required for cost-effective biological control. © 2013 Society of Chemical Industry.

  1. Effects of self-carbon dioxide-generation material for active packaging on pH, water-holding capacity, meat color, lipid oxidation and microbial growth in beef during cold storage.

    PubMed

    Lee, Seung-Jae; Lee, Seung Yun; Kim, Gap-Don; Kim, Geun-Bae; Jin, Sang Keun; Hur, Sun Jin

    2017-08-01

    Active packaging refers to the mixing of additive agents into packaging materials with the purpose of maintaining or extending food product quality and shelf life. The aim of this study was to develop an easy and cheap active packaging for beef. Beef loin samples were divided into three packaging groups (C, ziplock bag packaging; T1, vacuum packaging; T2, active packaging) and stored at 4 °C for 21 days. The water-holding capacity was significantly (P < 0.05) higher in C and T2 than in T1 for up to 7 days of storage. The TBARS value was significantly (P < 0.05) lower in T1 and T2 after 7 days of storage. The counts of some microorganism were significantly (P < 0.05) lower in T1 and T2 after 7 days of storage; the total bacterial count and Escherichia coli count were lowest in T2 at the end of storage. These results indicate that active packaging using self-CO 2 -generation materials can extend the shelf life similarly to that observed with vacuum packaging, and that the active packaging method can improve the quality characteristics of beef during cold storage. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  2. Valuing the Resilience Provided by Solar and Battery Energy Storage Systems

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McLaren, Joyce A; Mullendore, Seth; Laws, Nicholas D

    This paper explores the impact of valuing resilience on the economics of photovoltaics (PV) and storage systems for commercial buildings. The analysis presented here illustrates that accounting for the cost of grid power outages can change the breakeven point for PV and storage system investment, and increase the size of systems designed to deliver the greatest economic benefit over time. In other words, valuing resilience can make PV and storage systems economical in cases where they would not be otherwise. As storage costs decrease, and outages occur more frequently, PV and storage are likely to play a larger role inmore » building design and management considerations.« less

  3. Food safety hazards lurk in the kitchens of young adults.

    PubMed

    Byrd-Bredbenner, Carol; Maurer, Jaclyn; Wheatley, Virginia; Cottone, Ellen; Clancy, Michele

    2007-04-01

    Food mishandling in home kitchens likely causes a significant amount of foodborne disease; however, little is known about the food safety hazards lurking in home kitchens. The purposes of this study were to audit the kitchens of young adults with education beyond high school to identify food safety problems and develop recommendations for education efforts. Researchers developed a criterion-referenced home kitchen observation instrument to assess compliance of home food storage and rotation practices (e.g., temperature), sanitation and chemical storage, and general kitchen condition (e.g., infestation) with recommended practices. The instrument contained seven scales: Kitchen Cleanliness (eight items), Appliance Cleanliness (three items), Cleaning Supplies Availability (eight items), Temperatures (Food Thermometer Access & Refrigerator/Freezer Temperatures) (five items), Cold Food Storage (seven items), Dry Food Storage (eight items), and Poisons Storage (two items). Descriptive statistics were conducted to describe the study population, as a whole, and by gender. A total of 154 young adults (mean age, 20.7+/- 1.3 SD) enrolled in a northeastern university participated. Participants scored 70% or higher on Poisons Storage, Dry Food Storage, Kitchen Cleanliness, and Cleaning Supplies Availability scales but less than 60% on the Appliance Cleanliness and Cold Food Storage scales. Performance was lowest on the Temperatures scale. Females scored significantly higher than males on the Kitchen Cleanliness and Cleaning Supply Availability scales. Average refrigerator and freezer temperatures were higher than recommendations. Food safety education targeted at this young adult population needs to evolve into focused messages pertaining to the key food safety violations in this population.

  4. Regulation of sugar metabolism in wild-type and low-invertase transgenic chipping potatoes during and after cooling for low-temperature storage

    USDA-ARS?s Scientific Manuscript database

    Regulation of sugar metabolism in cold-stored potato tubers has significant ramifications for potato chip and French fry producers and consumers. Though low-temperature storage reduces losses due to sprouting and disease, it induces accumulation of the reducing sugars glucose and fructose. These rea...

  5. Temperature-dependent regulation of sugar metabolism in wild-type and low-invertase transgenic chipping potatoes during and after cooling for low-temperature storage

    USDA-ARS?s Scientific Manuscript database

    Regulation of sugar metabolism in cold-stored potato tubers has significant ramifications for potato chip and French fry producers and consumers. Though low-temperature storage reduces losses due to sprouting and disease, it induces accumulation of the reducing sugars glucose and fructose. These rea...

  6. Application of low concentrations of ozone during the cold storage of table grapes

    USDA-ARS?s Scientific Manuscript database

    The control of postharvest decay of table grapes, caused by Botrytis cinerea and other pathogens, by ozone was evaluated in chambers and commercial storage facilities. Ozone at 0.100 µL/L or higher inhibited the spread of gray mold among stored grapes. Ozone diffusion into many types of commercial p...

  7. Mandarin flavor and aroma volatile composition are strongly influenced by holding temperature

    USDA-ARS?s Scientific Manuscript database

    Mandarin flavor quality often declines during storage but the respective contributions to the flavor disorder of warm versus cold temperature portions of the storage regime were unknown. To determine this ‘W. Murcott’ mandarins were stored for either 6 weeks at a continuous 5 ºC or held at 20 ºC fo...

  8. ‘Golden Delicious’ and ‘Honeycrisp’ apple response to controlled atmosphere storage with oxygen set point determined in response to fruit chlorophyll fluorescence

    USDA-ARS?s Scientific Manuscript database

    Postharvest management of apple fruit ripening using controlled atmosphere (CA) cold storage can be enhanced as CA oxygen concentration is decreased to close to the anaerobic compensation point (ACP). Monitoring fruit chlorophyll fluorescence is one technology available to assess fruit response to ...

  9. Container Seedling Handling and Storage in the Southeastern States

    Treesearch

    Kasten R. Dumroese; James P. Barnett

    2004-01-01

    Most container seedlings grown in the southeastern US are outplanted during winter, although 10 to 20% are outplanted during summer. Longleaf pine accounts for more than 80% of all container seedlings produced. Very little information is published on cold hardiness and storage effects on container-grown southern pines and hardwoods. In general, growers attempt to...

  10. Dynamic-Type Ice Thermal Storage Systems

    NASA Astrophysics Data System (ADS)

    Ohira, Akiyoshi

    This paper deals with reviews for research and development of a dynamic-type ice thermal storage system. This system has three main features. First, the ice thermal storage tank and the ice generator are separate. Second, ice is transported to the tank from the ice generator by water or air. Third, the ice making and melting processes are operated at the same time. Outlet water temperature from the dynamic-type ice thermal storage tank remains low for a longer time. In this paper, dynamic-Type ice thermal storage systems are divided into three parts: the ice making part, the ice transport part, and the cold energy release part. Each part is reviewed separately.

  11. Measuring Device for Air Speed in Macroporous Media and Its Application Inside Apple Storage Bins

    PubMed Central

    Geyer, Martin; Praeger, Ulrike; Scaar, Holger; Neuwald, Daniel A.; Gottschalk, Klaus

    2018-01-01

    In cold storage facilities of fruit and vegetables, airflow is necessary for heat removal. The design of storage facilities influences the air speed in the surrounding of the product. Therefore, knowledge about airflow next to the product is important to plan the layout of cold stores adapted to the requirements of the products. A new sensing device (ASL, Air speed logger) is developed for omnidirectional measurement of air speed between fruit or vegetables inside storage bins or in bulk. It consists of four interconnected plastic spheres with 80 mm diameter each, adapted to the size of apple fruit. In the free space between the spheres, silicon diodes are fixed for the airflow measurement based on a calorimetric principle. Battery and data logger are mounted inside the spheres. The device is calibrated in a wind tunnel in a measuring range of 0–1.3 m/s. Air speed measurements in fruit bulks on laboratory scale and in an industrial fruit store show air speeds in gaps between fruit with high stability at different airflow levels. Several devices can be placed between stored products for determination of the air speed distribution inside bulks or bin stacks in a storage room. PMID:29438339

  12. 1. FACTORY BUILDING VIEWED FROM THE EAST, CURING AND STORAGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. FACTORY BUILDING VIEWED FROM THE EAST, CURING AND STORAGE WING TO THE LEFT, BOILING HOUSE IN THE CENTER, GRINDING PLATFORM TO THE FAR RIGHT, ST. THOMAS ISLAND VISIBLE IN BACKGROUND - Caneel Bay Plantation, Sugar Factory, Cruz Bay, St. John, VI

  13. MISCELLANEOUS SECTIONS AND DETAILS OF FUEL STORAGE BUILDING (CPP603). INL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    MISCELLANEOUS SECTIONS AND DETAILS OF FUEL STORAGE BUILDING (CPP-603). INL DRAWING NUMBER 200-0603-61-299-103032. ALTERNATE ID NUMBER 542-31-B-24. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  14. WEST ELEVATION OF FUEL STORAGE BUILDING (CPP603). PHOTO TAKEN LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEST ELEVATION OF FUEL STORAGE BUILDING (CPP-603). PHOTO TAKEN LOOKING NORTHEAST. INL PHOTO NUMBER HD-54-20-1. Mike Crane, Photographer, 8/2005 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  15. WEST ELEVATIONS AND SECTIONS OF FUEL STORAGE BUILDING (CPP603). INL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEST ELEVATIONS AND SECTIONS OF FUEL STORAGE BUILDING (CPP-603). INL DRAWING NUMBER 200-063-61-299-103031. ALTERNATE ID NUMBER 542-31-B-23. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  16. CONSTRUCTION PROGRESS PHOTO SHOWING EMPLACEMENT STEEL BEAMS FUEL STORAGE BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CONSTRUCTION PROGRESS PHOTO SHOWING EMPLACEMENT STEEL BEAMS FUEL STORAGE BUILDING (CPP-603) LOOKING EAST. INL PHOTO NUMBER NRTS-51-1371. Unknown Photographer, 1/31/1951 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  17. SIDING AND ROOF DETAILS OF FUEL STORAGE BUILDING (CPP603). INL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SIDING AND ROOF DETAILS OF FUEL STORAGE BUILDING (CPP-603). INL DRAWING NUMBER 200-0603-61-299-103033. ALTERNATE ID NUMBER 542-31-B-25. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  18. INTERIOR VIEW OF FUEL STORAGE BUILDING (CPP603) SHOWING CRANE ASSEMBLY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR VIEW OF FUEL STORAGE BUILDING (CPP-603) SHOWING CRANE ASSEMBLY FOR TRANSFER PIT. INL PHOTO NUMBER NRTS-51-2404. Unknown Photographer, 5/31/1951 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  19. WEST ELEVATION OF FUEL STORAGE BUILDING (CPP603). PHOTO TAKEN LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEST ELEVATION OF FUEL STORAGE BUILDING (CPP-603). PHOTO TAKEN LOOKING NORTHEAST. INL PHOTO NUMBER HD-54-20-3. Mike Crane, Photographer, 8/2005 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

  20. HOT CELL BUILDING, TRA632. CONTEXTUAL AERIAL VIEW OF HOT CELL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    HOT CELL BUILDING, TRA-632. CONTEXTUAL AERIAL VIEW OF HOT CELL BUILDING, IN VIEW AT LEFT, AS YET WITHOUT ROOF. PLUG STORAGE BUILDING LIES BETWEEN IT AND THE SOUTH SIDE OF THE MTR BUILDING AND ITS WING. NOTE CONCRETE DRIVE BETWEEN ROLL-UP DOOR IN MTR BUILDING AND CHARGING FACE OF PLUG STORAGE. REACTOR SERVICES BUILDING (TRA-635) WILL COVER THIS DRIVE AND BUTT UP TO CHARGING FACE. DOTTED LINE IS ON ORIGINAL NEGATIVE. TRA PARKING LOT IN LEFT CORNER OF THE VIEW. CAMERA FACING NORTHWESTERLY. INL NEGATIVE NO. 8274. Unknown Photographer, 7/2/1953 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

Top