Heterodyne frequency-domain multispectral diffuse optical tomography of breast cancer in the parallel-plane transmission geometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ban, H. Y.; Kavuri, V. C., E-mail: venk@physics.up

Purpose: The authors introduce a state-of-the-art all-optical clinical diffuse optical tomography (DOT) imaging instrument which collects spatially dense, multispectral, frequency-domain breast data in the parallel-plate geometry. Methods: The instrument utilizes a CCD-based heterodyne detection scheme that permits massively parallel detection of diffuse photon density wave amplitude and phase for a large number of source–detector pairs (10{sup 6}). The stand-alone clinical DOT instrument thus offers high spatial resolution with reduced crosstalk between absorption and scattering. Other novel features include a fringe profilometry system for breast boundary segmentation, real-time data normalization, and a patient bed design which permits both axial and sagittalmore » breast measurements. Results: The authors validated the instrument using tissue simulating phantoms with two different chromophore-containing targets and one scattering target. The authors also demonstrated the instrument in a case study breast cancer patient; the reconstructed 3D image of endogenous chromophores and scattering gave tumor localization in agreement with MRI. Conclusions: Imaging with a novel parallel-plate DOT breast imager that employs highly parallel, high-resolution CCD detection in the frequency-domain was demonstrated.« less

A New Remote Sensing Filter Radiometer Employing a Fabry-Perot Etalon and a CCD Camera for Column Measurements of Methane in the Earth Atmosphere

NASA Technical Reports Server (NTRS)

Georgieva, E. M.; Huang, W.; Heaps, W. S.

2012-01-01

A portable remote sensing system for precision column measurements of methane has been developed, built and tested at NASA GSFC. The sensor covers the spectral range from 1.636 micrometers to 1.646 micrometers, employs an air-gapped Fabry-Perot filter and a CCD camera and has a potential to operate from a variety of platforms. The detector is an XS-1.7-320 camera unit from Xenics Infrared solutions which combines an uncooled InGaAs detector array working up to 1.7 micrometers. Custom software was developed in addition to the graphical user basic interface X-Control provided by the company to help save and process the data. The technique and setup can be used to measure other trace gases in the atmosphere with minimal changes of the etalon and the prefilter. In this paper we describe the calibration of the system using several different approaches.

Development of a Portable 3CCD Camera System for Multispectral Imaging of Biological Samples

PubMed Central

Lee, Hoyoung; Park, Soo Hyun; Noh, Sang Ha; Lim, Jongguk; Kim, Moon S.

2014-01-01

Recent studies have suggested the need for imaging devices capable of multispectral imaging beyond the visible region, to allow for quality and safety evaluations of agricultural commodities. Conventional multispectral imaging devices lack flexibility in spectral waveband selectivity for such applications. In this paper, a recently developed portable 3CCD camera with significant improvements over existing imaging devices is presented. A beam-splitter prism assembly for 3CCD was designed to accommodate three interference filters that can be easily changed for application-specific multispectral waveband selection in the 400 to 1000 nm region. We also designed and integrated electronic components on printed circuit boards with firmware programming, enabling parallel processing, synchronization, and independent control of the three CCD sensors, to ensure the transfer of data without significant delay or data loss due to buffering. The system can stream 30 frames (3-waveband images in each frame) per second. The potential utility of the 3CCD camera system was demonstrated in the laboratory for detecting defect spots on apples. PMID:25350510

Does topical wound oxygen (TWO2) offer an improved outcome over conventional compression dressings (CCD) in the management of refractory venous ulcers (RVU)? A parallel observational comparative study.

PubMed

Tawfick, W; Sultan, S

2009-07-01

Topical wound oxygen (TWO(2)) may help wound healing in the management of refractory venous ulcers (RVU). The aim of this study was to measure the effect of TWO(2) on wound healing using the primary end-point of the proportion of ulcers healed at 12 weeks. Secondary end-points were time to full healing, percentage of reduction in ulcer size, pain reduction, recurrence rates and Quality-Adjusted Time Spent Without Symptoms of disease and Toxicity of Treatment (Q-TWiST). A parallel observational comparative study. Patients with CEAP C(6,s) RVU, assessed by duplex ultrasonography, were managed with either TWO(2) (n=46) or conventional compression dressings (CCD) (n=37) for 12 weeks or till full healing. Patients were followed up at 3 monthly intervals. At 12 weeks, 80% of TWO(2) managed ulcers were completely healed, compared to 35% of CCD ulcers (p<0.0001). Median time to full healing was 45 days in TWO(2) patients and 182 days in CCD patients (p<0.0001). The pain score threshold in TWO(2) managed patients improved from 8 to 3 by 13 days. After 12-month follow-up, 5 of the 13 healed CCD ulcers showed signs of recurrence compared to none of the 37 TWO(2) healed ulcers. TWO(2) patients experienced a significantly improved Q-TWiST. TWO(2) reduces recurrence rates, alleviates pain and improves the Q-TWiST. We believe it is a valuable tool in the armamentarium of management of RVU.

Two-column sequential injection chromatography--new approach for fast and effective analysis and its comparison with gradient elution chromatography.

PubMed

Chocholous, Petr; Satínský, Dalibor; Sklenárová, Hana; Solich, Petr

2010-05-23

This work presents novel approach in low-pressure chromatography flow systems--two-column Sequential Injection Chromatography (2-C SIC) and its comparison with gradient elution chromatography on the same instrument. The system was equipped with two different chromatographic columns (connected to selection valve in parallel design) for isocratic separation and determination of all components in composed anti-inflammatory pharmaceutical preparation (tablets). The sample was first injected on the first column of length 30 mm where less retained analytes were separated and then the sample was injected on the second column of length 10 mm where more retained analytes were separated. The SIC system was based on a commercial SIChrom manifold (8-port high-pressure selection valve and medium-pressure syringe pump with 4 mL reservoir) (FIAlab, USA) with two commercially available monolithic columns the "first column" Chromolith Flash RP-18e (25 mm x 4.6 mm i.d. with guard column 5 mm x 4.6 mm i.d.) and the "second column" Chromolith RP-18e (10 mm x 4.6 mm i.d.) and CCD UV-vis detector USB 4000 with micro-volume 1.0 cm Z flow cell. Two mobile phases were used for analysis (one for each column). The mobile phase 1 used for elution of paracetamol, caffeine and salicylic acid (internal standard) was acetonitrile/water (10:90, v/v, the water part of pH 3.5 adjusted with acetic acid), flow rate was 0.9 mL min(-1) (volume 3.0 mL of mobile phase per analysis). The mobile phase 2 used for elution of propyphenazone was acetonitrile/water (30:70, v/v); flow rate was 1.2 mL min(-1) (volume 1.5 mL of mobile phase per analysis). Absorbance was monitored at 210 nm. Samples were prepared by dissolving of one tablet in 30% acetonitrile and 10 microL of filtered supernatant was injected on each column (2 x 10 microL). The chromatographic resolution between all compounds was >1.45 and analysis time was 5.5 min under the optimal conditions. Limits of detection were determined at 0.4 microg mL(-1) for paracetamol, at 0.5 microg mL(-1) for caffeine and at 0.7 microg mL(-1) for propyphenazone. The new two-column chromatographic set-up developed as an alternative approach to gradient elution chromatography shows evident advantages (time and solvent reduction more than one-third) as compared with single-column gradient SIC method with Chromolith Flash RP-18 (25 mm x 4.6 mm i.d. with guard column 5 mm x 4.6 mm i.d.). Copyright 2010 Elsevier B.V. All rights reserved.

Characterization of a parallel beam CCD optical-CT apparatus for 3D radiation dosimetry

NASA Astrophysics Data System (ADS)

Krstajić, Nikola; Doran, Simon J.

2006-12-01

This paper describes the initial steps we have taken in establishing CCD based optical-CT as a viable alternative for 3-D radiation dosimetry. First, we compare the optical density (OD) measurements from a high quality test target and variable neutral density filter (VNDF). A modulation transfer function (MTF) of individual projections is derived for three positions of the sinusoidal test target within the scanning tank. Our CCD is then characterized in terms of its signal-to-noise ratio (SNR). Finally, a sample reconstruction of a scan of a PRESAGETM (registered trademark of Heuris Pharma, NJ, Skillman, USA.) dosimeter is given, demonstrating the capabilities of the apparatus.

A wide bandwidth CCD buffer memory system

NASA Technical Reports Server (NTRS)

Siemens, K.; Wallace, R. W.; Robinson, C. R.

1978-01-01

A prototype system was implemented to demonstrate that CCD's can be applied advantageously to the problem of low power digital storage and particularly to the problem of interfacing widely varying data rates. CCD shift register memories (8K bit) were used to construct a feasibility model 128 K-bit buffer memory system. Serial data that can have rates between 150 kHz and 4.0 MHz can be stored in 4K-bit, randomly-accessible memory blocks. Peak power dissipation during a data transfer is less than 7 W, while idle power is approximately 5.4 W. The system features automatic data input synchronization with the recirculating CCD memory block start address. System expansion to accommodate parallel inputs or a greater number of memory blocks can be performed in a modular fashion. Since the control logic does not increase proportionally to increase in memory capacity, the power requirements per bit of storage can be reduced significantly in a larger system.

A 780 × 800 μm2 Multichannel Digital Silicon Photomultiplier With Column-Parallel Time-to-Digital Converter and Basic Characterization

NASA Astrophysics Data System (ADS)

Mandai, Shingo; Jain, Vishwas; Charbon, Edoardo

2014-02-01

This paper presents a digital silicon photomultiplier (SiPM) partitioned in columns, whereas each column is connected to a column-parallel time-to-digital converter (TDC), in order to improve the timing resolution of single-photon detection. By reducing the number of pixels per TDC using a sharing scheme with three TDCs per column, the pixel-to-pixel skew is reduced. We report the basic characterization of the SiPM, comprising 416 single-photon avalanche diodes (SPADs); the characterization includes photon detection probability, dark count rate, afterpulsing, and crosstalk. We achieved 264-ps full-width at half maximum timing resolution of single-photon detection using a 48-fold column-parallel TDC with a temporal resolution of 51.8 ps (least significant bit), fully integrated in standard complementary metal-oxide semiconductor technology.

A parallel bubble column system for the cultivation of phototrophic microorganisms.

PubMed

Havel, Jan; Franco-Lara, Ezequiel; Weuster-Botz, Dirk

2008-07-01

An incubator with up to 16 parallel bubble columns was equipped with artificial light sources assuring a light supply with a homogenous light spectrum directly above the bioreactors. Cylindrical light reflecting tubes were positioned around every single bubble column to avoid light scattering effects and to redirect the light from the top onto the cylindrical outer glass surface of each bubble column. The light reflecting tubes were equipped with light intensity filters to control the total light intensity for every single photo-bioreactor. Parallel cultivations of the unicellular obligate phototrophic cyanobacterium, Synechococcus PCC7942, were studied under different constant light intensities ranging from 20 to 102 microE m(-2)s(-1) at a constant humidified air flow rate supplemented with CO(2).

"Cloud Slicing" : A New Technique to Derive Tropospheric Ozone Profile Information from Satellite Measurements

NASA Technical Reports Server (NTRS)

Ziemke, J. R.; Chandra, S.; Bhartia, P. K.; Einaudi, Franco (Technical Monitor)

2000-01-01

A new technique denoted cloud slicing has been developed for estimating tropospheric ozone profile information. All previous methods using satellite data were only capable of estimating the total column of ozone in the troposphere. Cloud slicing takes advantage of the opaque property of water vapor clouds to ultraviolet wavelength radiation. Measurements of above-cloud column ozone from the Nimbus 7 total ozone mapping spectrometer (TOMS) instrument are combined together with Nimbus 7 temperature humidity and infrared radiometer (THIR) cloud-top pressure data to derive ozone column amounts in the upper troposphere. In this study tropical TOMS and THIR data for the period 1979-1984 are analyzed. By combining total tropospheric column ozone (denoted TCO) measurements from the convective cloud differential (CCD) method with 100-400 hPa upper tropospheric column ozone amounts from cloud slicing, it is possible to estimate 400-1000 hPa lower tropospheric column ozone and evaluate its spatial and temporal variability. Results for both the upper and lower tropical troposphere show a year-round zonal wavenumber 1 pattern in column ozone with largest amounts in the Atlantic region (up to approx. 15 DU in the 100-400 hPa pressure band and approx. 25-30 DU in the 400-1000 hPa pressure band). Upper tropospheric ozone derived from cloud slicing shows maximum column amounts in the Atlantic region in the June-August and September-November seasons which is similar to the seasonal variability of CCD derived TCO in the region. For the lower troposphere, largest column amounts occur in the September-November season over Brazil in South America and also southern Africa. Localized increases in the tropics in lower tropospheric ozone are found over the northern region of South America around August and off the west coast of equatorial Africa in the March-May season. Time series analysis for several regions in South America and Africa show an anomalous increase in ozone in the lower troposphere around the month of March which is not observed in the upper troposphere. The eastern Pacific indicates weak seasonal variability of upper, lower, and total tropospheric ozone compared to the western Pacific which shows largest TCO amounts in both hemispheres around spring months. Ozone variability in the western Pacific is expected to have greater variability caused by strong convection, pollution and biomass burning, land/sea contrast and monsoon developments.

64 x 64 thresholding photodetector array for optical pattern recognition

NASA Astrophysics Data System (ADS)

Langenbacher, Harry; Chao, Tien-Hsin; Shaw, Timothy; Yu, Jeffrey W.

1993-10-01

A high performance 32 X 32 peak detector array is introduced. This detector consists of a 32 X 32 array of thresholding photo-transistor cells, manufactured with a standard MOSIS digital 2-micron CMOS process. A built-in thresholding function that is able to perform 1024 thresholding operations in parallel strongly distinguishes this chip from available CCD detectors. This high speed detector offers responses from one to 10 milliseconds that is much higher than the commercially available CCD detectors operating at a TV frame rate. The parallel multiple peaks thresholding detection capability makes it particularly suitable for optical correlator and optoelectronically implemented neural networks. The principle of operation, circuit design and the performance characteristics are described. Experimental demonstration of correlation peak detection is also provided. Recently, we have also designed and built an advanced version of a 64 X 64 thresholding photodetector array chip. Experimental investigation of using this chip for pattern recognition is ongoing.

A CMOS In-Pixel CTIA High Sensitivity Fluorescence Imager.

PubMed

Murari, Kartikeya; Etienne-Cummings, Ralph; Thakor, Nitish; Cauwenberghs, Gert

2011-10-01

Traditionally, charge coupled device (CCD) based image sensors have held sway over the field of biomedical imaging. Complementary metal oxide semiconductor (CMOS) based imagers so far lack sensitivity leading to poor low-light imaging. Certain applications including our work on animal-mountable systems for imaging in awake and unrestrained rodents require the high sensitivity and image quality of CCDs and the low power consumption, flexibility and compactness of CMOS imagers. We present a 132×124 high sensitivity imager array with a 20.1 μm pixel pitch fabricated in a standard 0.5 μ CMOS process. The chip incorporates n-well/p-sub photodiodes, capacitive transimpedance amplifier (CTIA) based in-pixel amplification, pixel scanners and delta differencing circuits. The 5-transistor all-nMOS pixel interfaces with peripheral pMOS transistors for column-parallel CTIA. At 70 fps, the array has a minimum detectable signal of 4 nW/cm(2) at a wavelength of 450 nm while consuming 718 μA from a 3.3 V supply. Peak signal to noise ratio (SNR) was 44 dB at an incident intensity of 1 μW/cm(2). Implementing 4×4 binning allowed the frame rate to be increased to 675 fps. Alternately, sensitivity could be increased to detect about 0.8 nW/cm(2) while maintaining 70 fps. The chip was used to image single cell fluorescence at 28 fps with an average SNR of 32 dB. For comparison, a cooled CCD camera imaged the same cell at 20 fps with an average SNR of 33.2 dB under the same illumination while consuming over a watt.

A CMOS In-Pixel CTIA High Sensitivity Fluorescence Imager

PubMed Central

Murari, Kartikeya; Etienne-Cummings, Ralph; Thakor, Nitish; Cauwenberghs, Gert

2012-01-01

Traditionally, charge coupled device (CCD) based image sensors have held sway over the field of biomedical imaging. Complementary metal oxide semiconductor (CMOS) based imagers so far lack sensitivity leading to poor low-light imaging. Certain applications including our work on animal-mountable systems for imaging in awake and unrestrained rodents require the high sensitivity and image quality of CCDs and the low power consumption, flexibility and compactness of CMOS imagers. We present a 132×124 high sensitivity imager array with a 20.1 μm pixel pitch fabricated in a standard 0.5 μ CMOS process. The chip incorporates n-well/p-sub photodiodes, capacitive transimpedance amplifier (CTIA) based in-pixel amplification, pixel scanners and delta differencing circuits. The 5-transistor all-nMOS pixel interfaces with peripheral pMOS transistors for column-parallel CTIA. At 70 fps, the array has a minimum detectable signal of 4 nW/cm2 at a wavelength of 450 nm while consuming 718 μA from a 3.3 V supply. Peak signal to noise ratio (SNR) was 44 dB at an incident intensity of 1 μW/cm2. Implementing 4×4 binning allowed the frame rate to be increased to 675 fps. Alternately, sensitivity could be increased to detect about 0.8 nW/cm2 while maintaining 70 fps. The chip was used to image single cell fluorescence at 28 fps with an average SNR of 32 dB. For comparison, a cooled CCD camera imaged the same cell at 20 fps with an average SNR of 33.2 dB under the same illumination while consuming over a watt. PMID:23136624

Carbonate dissolution in the South Atlantic Ocean: evidence from ultrastructure breakdown in Globigerina bulloides

NASA Astrophysics Data System (ADS)

Dittert, Nicolas; Henrich, Rüdiger

2000-04-01

Ultrastructure dissolution susceptibility of the planktic foraminifer Globigerina bulloides, carbonate ion content of the water column, calcium carbonate content of the sediment surface, and carbonate/carbon weight percentage ratio derived from sediment surface samples were investigated in order to reconstruct the position of the calcite saturation horizon, the sedimentary calcite lysocline, and the calcium carbonate compensation depth (CCD) in the modern South Atlantic Ocean. Carbonate ion data from the water column refer to the GEOSECS locations 48, 103, and 109 and calcium carbonate data come from 19 GeoB sediment surface samples of 4 transects into the Brazil, the Guinea, and the Cape Basins. We present a new (paleo-) oceanographic tool, namely the Globigerina bulloides dissolution index (BDX). Further, we give evidence (a) for progressive G. bulloides ultrastructural breakdown with increasing carbonate dissolution even above the lysocline; (b) for a sharp BDX increase at the sedimentary lysocline; and (c) for the total absence of this species at the CCD. BDX puts us in the position to distinguish the upper open ocean and the upwelling influenced continental margin above from the deep ocean below the sedimentary lysocline. Carbonate ion data from water column samples, calcite weight percentage data from surface sediment samples, and carbonate/carbon weight percentage ratio appear to be good proxies to confirm BDX. As shown by BDX both the calcite saturation horizon (in the water column) and the sedimentary lysocline (at the sediment-water interface) mark the boundary between the carbonate ion undersaturated and highly corrosive Antarctic Bottom Water and the carbonate ion saturated North Atlantic Deep Water (NADW) of the modern South Atlantic.

Modeling of the adsorptive removal of arsenic(III) using plant biomass: a bioremedial approach

NASA Astrophysics Data System (ADS)

Roy, Palas; Dey, Uttiya; Chattoraj, Soumya; Mukhopadhyay, Debasis; Mondal, Naba Kumar

2017-06-01

In the present work, the possibility of using a non-conventional finely ground (250 μm) Azadirachta indica (neem) bark powder [AiBP] has been tested as a low-cost biosorbent for the removal of arsenic(III) from water. The removal of As(III) was studied by performing a series of biosorption experiments (batch and column). The biosorption behavior of As(III) for batch and column operations were examined in the concentration ranges of 50-500 µg L-1 and 500.0-2000.0 µg L-1, respectively. Under optimized batch conditions, the AiBP could remove up to 89.96 % of As(III) in water system. The artificial neural network (ANN) model was developed from batch experimental data sets which provided reasonable predictive performance ( R 2 = 0.961; 0.954) of As(III) biosorption. In batch operation, the initial As(III) concentration had the most significant impact on the biosorption process. For column operation, central composite design (CCD) was applied to investigate the influence on the breakthrough time for optimization of As(III) biosorption process and evaluation of interacting effects of different operating variables. The optimized result of CCD revealed that the AiBP was an effective and economically feasible biosorbent with maximum breakthrough time of 653.9 min, when the independent variables were retained at 2.0 g AiBP dose, 2000.0 µg L-1 initial As(III) concentrations, and 3.0 mL min-1 flow rate, at maximum desirability value of 0.969.

Measurements of stratospheric composition using a star pointing spectrometer

NASA Technical Reports Server (NTRS)

Fish, Deb J.; Jones, Rod L.; Freshwater, Ray A.; Roscoe, Howard K.; Oldham, Derek J.

1994-01-01

Measurements of stratospheric composition have been made with a novel star-pointing spectrometer. The instrument consists of a telescope that focuses light from stars, planets, or the moon onto a spectrometer and two dimensional CCD array detector. Atmospheric absorptions can be measured, from which atmospheric columns of several gases can be determined. The instrument was deployed in Abisko, 69 deg N, during the European Arctic Stratospheric Ozone Experiment (EASOE). The instrument has the potential for measuring O3, OClO, NO2, and NO3. In this paper, a method for the retrieval of vertical columns is described, and some examples of ozone measurements given.

Multi-Probe SPM using Interference Patterns for a Parallel Nano Imaging

NASA Astrophysics Data System (ADS)

Koyama, Hirotaka; Oohira, Fumikazu; Hosogi, Maho; Hashiguchi, Gen

This paper proposes a new composition of the multi-probe using optical interference patterns for a parallel nano imaging in a large area scanning. We achieved large-scale integration with 50,000 probes fabricated with MEMS technology, and measured the optical interference patterns with CCD, which was difficult in a conventional single scanning probe. In this research, the multi-probes are made of Si3N4 by MEMS process, and, the multi-probes are joined with a Pyrex glass by an anodic bonding. We designed, fabricated, and evaluated the characteristics of the probe. In addition, we changed the probe shape to decrease the warpage of the Si3N4 probe. We used the supercritical drying to avoid stiction of the Si3N4 probe with the glass surface and fabricated 4 types of the probe shapes without stiction. We took some interference patterns by CCD and measured the position of them. We calculate the probe height using the interference displacement and compared the result with the theoretical deflection curve. As a result, these interference patterns matched the theoretical deflection curve. We found that this multi-probe chip using interference patterns is effective in measurement for a parallel nano imaging.

Aerosol mobility imaging for rapid size distribution measurements

DOEpatents

Wang, Jian; Hering, Susanne Vera; Spielman, Steven Russel; Kuang, Chongai

2016-07-19

A parallel plate dimensional electrical mobility separator and laminar flow water condensation provide rapid, mobility-based particle sizing at concentrations typical of the remote atmosphere. Particles are separated spatially within the electrical mobility separator, enlarged through water condensation, and imaged onto a CCD array. The mobility separation distributes particles in accordance with their size. The condensation enlarges size-separated particles by water condensation while they are still within the gap of the mobility drift tube. Once enlarged the particles are illuminated by a laser. At a pre-selected frequency, typically 10 Hz, the position of all of the individual particles illuminated by the laser are captured by CCD camera. This instantly records the particle number concentration at each position. Because the position is directly related to the particle size (or mobility), the particle size spectra is derived from the images recorded by the CCD.

Cometary activity in 2060 Chiron

NASA Technical Reports Server (NTRS)

Luu, Jane X.; Jewitt, David C.

1990-01-01

Results of a 2-yr (1988-90) investigation of cometary activity in 2060 Chiron based on CCD photometry and spectroscopy are reported. The photometry observations include a new rotational light curve of Chiron, a newly refined rotation period, recent developments of its long-term photometric behavior and surface brightness profiles, a deep image of the coma of Chiron, and narrowband images at wavelengths ranging from 3200 to 6840 A. The spectroscopic data include moderate resolution CCD spectra (10-20 A FWHM). Major results include the detection of impulsive brightening on a time scale of hours, evidence for a secular change in the blue portion of the reflectivity spectrum of the nucleus, no evidence for Rayleigh scattering in the near ultraviolet, and an upper limit of the column density of CO(+) ions in the coma.

Optical registration of spaceborne low light remote sensing camera

NASA Astrophysics Data System (ADS)

Li, Chong-yang; Hao, Yan-hui; Xu, Peng-mei; Wang, Dong-jie; Ma, Li-na; Zhao, Ying-long

2018-02-01

For the high precision requirement of spaceborne low light remote sensing camera optical registration, optical registration of dual channel for CCD and EMCCD is achieved by the high magnification optical registration system. System integration optical registration and accuracy of optical registration scheme for spaceborne low light remote sensing camera with short focal depth and wide field of view is proposed in this paper. It also includes analysis of parallel misalignment of CCD and accuracy of optical registration. Actual registration results show that imaging clearly, MTF and accuracy of optical registration meet requirements, it provide important guarantee to get high quality image data in orbit.

Detection for flatness of large surface based on structured light

NASA Astrophysics Data System (ADS)

He, Wenyan; Cao, Xuedong; Long, Kuang; Peng, Zhang

2016-09-01

In order to get flatness of a large plane, this paper set up a measurement system, composed by Line Structured Light, imaging system, CCD, etc. Line Structured Light transmits parallel fringes at a proper angle onto the plane which is measured; the imaging system and CCD locate above the plane to catch the fringes. When the plane is perfect, CCD will catch straight fringes; however, the real plane is not perfect; according to the theory of projection, the fringes caught by CCD will be distorted by convex and concave. Extract the center of line fringes to obtain the distortion of the fringe, according to the functional relationship between the distortion of fringes and the height which is measured, then we will get flatness of the entire surface. Data from experiment approached the analysis of theory. In the simulation, the vertical resolution is 0.0075 mm per pixel when measuring a plane of 400mm×400mm, choosing the size of CCD 4096×4096, at the angle 85°. Helped by sub-pixel, the precision will get the level of submicron. There are two obvious advantages: method of surface sampling can increase the efficiency for auto-repairing of machines; considering the center of fringe is required mainly in this system, as a consequence, there is no serious demand for back light.

Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser-induced fluorescence detection.

PubMed

Nikcevic, Irena; Piruska, Aigars; Wehmeyer, Kenneth R; Seliskar, Carl J; Limbach, Patrick A; Heineman, William R

2010-08-01

Parallel separations using CE on a multilane microchip with multiplexed LIF detection is demonstrated. The detection system was developed to simultaneously record data on all channels using an expanded laser beam for excitation, a camera lens to capture emission, and a CCD camera for detection. The detection system enables monitoring of each channel continuously and distinguishing individual lanes without significant crosstalk between adjacent lanes. Multiple analytes can be determined in parallel lanes within a single microchip in a single run, leading to increased sample throughput. The pK(a) determination of small molecule analytes is demonstrated with the multilane microchip.

Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser induced fluorescence detection

PubMed Central

Nikcevic, Irena; Piruska, Aigars; Wehmeyer, Kenneth R.; Seliskar, Carl J.; Limbach, Patrick A.; Heineman, William R.

2010-01-01

Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser induced fluorescence detection is demonstrated. The detection system was developed to simultaneously record data on all channels using an expanded laser beam for excitation, a camera lens to capture emission, and a CCD camera for detection. The detection system enables monitoring of each channel continuously and distinguishing individual lanes without significant crosstalk between adjacent lanes. Multiple analytes can be analyzed on parallel lanes within a single microchip in a single run, leading to increased sample throughput. The pKa determination of small molecule analytes is demonstrated with the multilane microchip. PMID:20737446

Microfluidic integration of parallel solid-phase liquid chromatography.

PubMed

Huft, Jens; Haynes, Charles A; Hansen, Carl L

2013-03-05

We report the development of a fully integrated microfluidic chromatography system based on a recently developed column geometry that allows for robust packing of high-performance separation columns in poly(dimethylsiloxane) microfluidic devices having integrated valves made by multilayer soft lithography (MSL). The combination of parallel high-performance separation columns and on-chip plumbing was used to achieve a fully integrated system for on-chip chromatography, including all steps of automated sample loading, programmable gradient generation, separation, fluorescent detection, and sample recovery. We demonstrate this system in the separation of fluorescently labeled DNA and parallel purification of reverse transcription polymerase chain reaction (RT-PCR) amplified variable regions of mouse immunoglobulin genes using a strong anion exchange (AEX) resin. Parallel sample recovery in an immiscible oil stream offers the advantage of low sample dilution and high recovery rates. The ability to perform nucleic acid size selection and recovery on subnanogram samples of DNA holds promise for on-chip genomics applications including sequencing library preparation, cloning, and sample fractionation for diagnostics.

A New Serial-direction Trail Effect in CCD Images of the Lunar-based Ultraviolet Telescope

NASA Astrophysics Data System (ADS)

Wu, C.; Deng, J. S.; Guyonnet, A.; Antilogus, P.; Cao, L.; Cai, H. B.; Meng, X. M.; Han, X. H.; Qiu, Y. L.; Wang, J.; Wang, S.; Wei, J. Y.; Xin, L. P.; Li, G. W.

2016-10-01

Unexpected trails have been seen subsequent to relative bright sources in astronomical images taken with the CCD camera of the Lunar-based Ultraviolet Telescope (LUT) since its first light on the Moon’s surface. The trails can only be found in the serial-direction of CCD readout, differing themselves from image trails of radiation-damaged space-borne CCDs, which usually appear in the parallel-readout direction. After analyzing the same trail defects following warm pixels (WPs) in dark frames, we found that the relative intensity profile of the LUT CCD trails can be expressed as an exponential function of the distance i (in number of pixels) of the trailing pixel to the original source (or WP), i.e., {\\mathtt{\\exp }}(α {\\mathtt{i}}+β ). The parameters α and β seem to be independent of the CCD temperature, intensity of the source (or WP), and its position in the CCD frame. The main trail characteristics show evolution occurring at an increase rate of ˜(7.3 ± 3.6) × 10-4 in the first two operation years. The trails affect the consistency of the profiles of different brightness sources, which make smaller aperture photometry have larger extra systematic error. The astrometric uncertainty caused by the trails is too small to be acceptable based on LUT requirements for astrometry accuracy. Based on the empirical profile model, a correction method has been developed for LUT images that works well for restoring the fluxes of astronomical sources that are lost in trailing pixels.

Cometary activity in 2060 Chiron

DOE Office of Scientific and Technical Information (OSTI.GOV)

Luu, J.X.; Jewitt, D.C.

Results of a 2-yr (1988-90) investigation of cometary activity in 2060 Chiron based on CCD photometry and spectroscopy are reported. The photometry observations include a new rotational light curve of Chiron, a newly refined rotation period, recent developments of its long-term photometric behavior and surface brightness profiles, a deep image of the coma of Chiron, and narrowband images at wavelengths ranging from 3200 to 6840 A. The spectroscopic data include moderate resolution CCD spectra (10-20 A FWHM). Major results include the detection of impulsive brightening on a time scale of hours, evidence for a secular change in the blue portionmore » of the reflectivity spectrum of the nucleus, no evidence for Rayleigh scattering in the near ultraviolet, and an upper limit of the column density of CO(+) ions in the coma. 46 refs.« less

National Combustion Code: Parallel Implementation and Performance

NASA Technical Reports Server (NTRS)

Quealy, A.; Ryder, R.; Norris, A.; Liu, N.-S.

2000-01-01

The National Combustion Code (NCC) is being developed by an industry-government team for the design and analysis of combustion systems. CORSAIR-CCD is the current baseline reacting flow solver for NCC. This is a parallel, unstructured grid code which uses a distributed memory, message passing model for its parallel implementation. The focus of the present effort has been to improve the performance of the NCC flow solver to meet combustor designer requirements for model accuracy and analysis turnaround time. Improving the performance of this code contributes significantly to the overall reduction in time and cost of the combustor design cycle. This paper describes the parallel implementation of the NCC flow solver and summarizes its current parallel performance on an SGI Origin 2000. Earlier parallel performance results on an IBM SP-2 are also included. The performance improvements which have enabled a turnaround of less than 15 hours for a 1.3 million element fully reacting combustion simulation are described.

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection.

PubMed

Pravadali-Cekic, Sercan; Kocic, Danijela; Hua, Stanley; Jones, Andrew; Dennis, Gary R; Shalliker, R Andrew

2015-12-15

Active flow technology (AFT) is new form of column technology that was designed to overcome flow heterogeneity to increase separation performance in terms of efficiency and sensitivity and to enable multiplexed detection. This form of AFT uses a parallel segmented flow (PSF) column. A PSF column outlet end-fitting consists of 2 or 4 ports, which can be multiplexed to connect up to 4 detectors. The PSF column not only allows a platform for multiplexed detection but also the combination of both destructive and non-destructive detectors, without additional dead volume tubing, simultaneously. The amount of flow through each port can also be adjusted through pressure management to suit the requirements of a specific detector(s). To achieve multiplexed detection using a PSF column there are a number of parameters which can be controlled to ensure optimal separation performance and quality of results; that is tube dimensions for each port, choice of port for each type of detector and flow adjustment. This protocol is intended to show how to use and tune a PSF column functioning in a multiplexed mode of detection.

Occultation Predictions Using CCD Strip-Scanning Astrometry

NASA Technical Reports Server (NTRS)

Dunham, Edward W.; Ford, C. H.; Stone, R. P. S.; McDonald, S. W.; Olkin, C. B.; Elliot, J. L.; Witteborn, Fred C. (Technical Monitor)

1994-01-01

We are developing the method of CCD strip-scanning astrometry for the purpose of deriving reliable advance predictions for occultations involving small objects in the outer solar system. We are using a camera system based on a Ford/Loral 2Kx2K CCD with the Crossley telescope at Lick Observatory for this work. The columns of die CCD are aligned East-West, the telescope drive is stopped, and the CCD is clocked at the same rate that the stars drift across it. In this way we obtain arbitrary length strip images 20 arcmin wide with 0.58" pixels. Since planets move mainly in RA, it is possible to obtain images of the planet and star to be occulted on the same strip well before the occultation occurs. The strip-to-strip precision (i.e. reproducibility) of positions is limited by atmospheric image motion to about 0.1" rms per strip. However, for objects that are nearby in R.A., the image motion is highly correlated and their relative positions are good to 0.02" rms per strip. We will show that the effects of atmospheric image motion on a given strip can be removed if a sufficient number of strips of a given area have been obtained. Thus, it is possible to reach an rms precision of 0.02" per strip, corresponding to about 0.3 of Pluto or Triton's angular radius. The ultimate accuracy of a prediction based on strip-scanning astrometry is currently limited by the accuracy of the positions of the stars in the astrometric network used and by systematic errors most likely due to the optical system. We will show the results of . the prediction of some recent occultations as examples of the current capabilities and limitations of this technique.

An LOD with improved breakdown voltage in full-frame CCD devices

NASA Astrophysics Data System (ADS)

Banghart, Edmund K.; Stevens, Eric G.; Doan, Hung Q.; Shepherd, John P.; Meisenzahl, Eric J.

2005-02-01

In full-frame image sensors, lateral overflow drain (LOD) structures are typically formed along the vertical CCD shift registers to provide a means for preventing charge blooming in the imager pixels. In a conventional LOD structure, the n-type LOD implant is made through the thin gate dielectric stack in the device active area and adjacent to the thick field oxidation that isolates the vertical CCD columns of the imager. In this paper, a novel LOD structure is described in which the n-type LOD impurities are placed directly under the field oxidation and are, therefore, electrically isolated from the gate electrodes. By reducing the electrical fields that cause breakdown at the silicon surface, this new structure permits a larger amount of n-type impurities to be implanted for the purpose of increasing the LOD conductivity. As a consequence of the improved conductance, the LOD width can be significantly reduced, enabling the design of higher resolution imaging arrays without sacrificing charge capacity in the pixels. Numerical simulations with MEDICI of the LOD leakage current are presented that identify the breakdown mechanism, while three-dimensional solutions to Poisson's equation are used to determine the charge capacity as a function of pixel dimension.

Upgrading the Arecibo Potassium Lidar Receiver for Meridional Wind Measurements

NASA Astrophysics Data System (ADS)

Piccone, A. N.; Lautenbach, J.

2017-12-01

Lidar can be used to measure a plethora of variables: temperature, density of metals, and wind. This REU project is focused on the set up of a semi steerable telescope that will allow the measurement of meridional wind in the mesosphere (80-105 km) with Arecibo Observatory's potassium resonance lidar. This includes the basic design concept of a steering system that is able to turn the telescope to a maximum of 40°, alignment of the mirror with the telescope frame to find the correct focusing, and the triggering and programming of a CCD camera. The CCD camera's purpose is twofold: looking though the telescope and matching the stars in the field of view with a star map to accurately calibrate the steering system and determining the laser beam properties and position. Using LabVIEW, the frames from the CCD camera can be analyzed to identify the most intense pixel in the image (and therefore the brightest point in the laser beam or stars) by plotting average pixel values per row and column and locating the peaks of these plots. The location of this pixel can then be plotted, determining the jitter in the laser and position within the field of view of the telescope.

pnCCD for photon detection from near-infrared to X-rays

NASA Astrophysics Data System (ADS)

Meidinger, Norbert; Andritschke, Robert; Hartmann, Robert; Herrmann, Sven; Holl, Peter; Lutz, Gerhard; Strüder, Lothar

2006-09-01

A pnCCD is a special type of charge-coupled device developed for spectroscopy and imaging of X-rays with high time resolution and quantum efficiency. Its most famous application is the operation on the XMM-Newton satellite, an X-ray astronomy mission that was launched by the European space agency in 1999. The excellent performance of the focal plane camera has been maintained for more than 6 years in orbit. The energy resolution in particular has shown hardly any degradation since launch. In order to satisfy the requirements of future X-ray astronomy missions as well as those of ground-based experiments, a new type of pnCCD has been developed. This ‘frame-store pnCCD’ shows an enhanced performance compared to the XMM-Newton type of pnCCD. Now, more options in device design and operation are available to tailor the detector to its respective application. Part of this concept is a programmable analog signal processor, which has been developed for the readout of the CCD signals. The electronic noise of the new detector has a value of only 2 electrons equivalent noise charge (ENC), which is less than half of the figure achieved for the XMM-Newton-type pnCCD. The energy resolution for the Mn-Kα line at 5.9 keV is approximately 130 eV FWHM. We have close to 100% quantum efficiency for both low- and high-energy photon detection (e.g. the C-K line at 277 eV, and the Ge-Kα line at 10 keV, respectively). Very high frame rates of 1000 images/s have been achieved due to the ultra-fast readout accomplished by the parallel architecture of the pnCCD and the analog signal processor. Excellent spectroscopic performance is shown even at the relatively high operating temperature of -25 °C that can be achieved by a Peltier cooler. The applications of the low-noise and fast pnCCD detector are not limited to the detection of X-rays. With an anti-reflective coating deposited on the photon entrance window, we achieve high quantum efficiency also for near-infrared and optical photons. A novel type of pnCCD is in preparation, which allows single optical photon counting. This feature is accomplished by implementation of an avalanche-type amplifier in the pnCCD concept.

Columnar Transitions in Microscale Evaporating Liquid Jets

NASA Astrophysics Data System (ADS)

Hunter, Hanif; Glezer, Ari

2007-11-01

Microscale evaporating liquid jets that are injected into a quiescent gaseous medium having adjustable ambient pressure are investigated over a range of jet speeds using a shadowgraph technique. The jets are formed by a laser-drilled 10 μm nozzle from a small-scale pressurized reservoir, and sub-atmospheric ambient pressure is maintained using a controllable, metered Venturi pump. The near-field jet features are captured by shadowgraph imaging using a pulsed ND-Yag laser and a 12 bit CCD camera where the field of view measured 200 μm on the side. As the ambient pressure is reduced, the jet column undergoes a series of spectacular transitions that are first marked by the appearance of vapor bubbles within the jet column. The transitions progress from columnar instabilities to series of column bifurcations to high-order branching and film formation and culminate in conical atomization of the jet column. In addition to the effects of the ambient pressure, the present investigation also considers effects of the liquid surface tension and vapor pressure on the onset, evolution, and hysteresis of the columnar transitions.

Parallel Group and Sunspot Counts from SDO/HMI and AAVSO Visual Observers (Abstract)

NASA Astrophysics Data System (ADS)

Howe, R.; Alvestad, J.

2015-06-01

(Abstract only) Creating group and sunspot counts from the SDO/HMI detector on the Solar Dynamics Observatory (SDO) satellite requires software that calculates sunspots from a “white light” intensity-gram (CCD image) and group counts from a filtered CCD magneto-gram. Images from the satellite come from here http://jsoc.stanford.edu/data/hmi/images/latest/ Together these two sets of images can be used to estimate the Wolf number as W = (10g + s), which is used to calculate the American Relative index. AAVSO now has approximately two years of group and sunspot counts in the SunEntry database as SDOH observer Jan Alvestad. It is important that we compare these satellite CCD image data with our visual observer daily submissions to determine if the SDO/HMI data should be included in calculating the American Relative index. These satellite data are continuous observations with excellent seeing. This contrasts with “snapshot” earth-based observations with mixed seeing. The SDO/HIM group and sunspot counts could be considered unbiased, except that they show a not normal statistical distribution when compared to the overall visual observations, which show a Poisson distribution. One challenge that should be addressed by AAVSO using these SDO/HMI data is the splitting of groups and deriving group properties from the magneto-grams. The filtered CCD detector that creates the magento-grams is not something our visual observers can relate too, unless they were to take CCD images in H-alpha and/or the Calcium spectrum line. So, questions remain as to how these satellite CCD image counts can be integrated into the overall American Relative index.

Quantitative measurements of magnetic vortices using position resolved diffraction in Lorentz STEM

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zaluzec, N. J.

2002-03-05

A number of electron column techniques have been developed over the last forty years to permit visualization of magnetic fields in specimens. These include: Fresnel imaging, Differential Phase Contrast, Electron Holography and Lorentz STEM. In this work we have extended the LSTEM methodology using Position Resolved Diffraction (PRD) to quantitatively measure the in-plane electromagnetic fields of thin film materials. The experimental work reported herein has been carried out using the ANL AAEM HB603Z 300 kV FEG instrument 5. In this instrument, the electron optical column was operated in a zero field mode, at the specimen, where the objective lens ismore » turned off and the probe forming lens functions were reallocated to the C1, C2, and C3 lenses. Post specimen lenses (P1, P2, P3, P4) were used to magnify the transmitted electrons to a YAG screen, which was then optically transferred to a Hamamatsu ORCA ER CCD array. This CCD was interfaced to an EmiSpec Data Acquisition System and the data was subsequently transferred to an external computer system for detailed quantitative analysis. In Position Resolved Diffraction mode, we digitally step a focused electron probe across the region of interest of the specimen while at the same time recording the complete diffraction pattern at each point in the scan.« less

The MUK five protocol: a phase II randomised, controlled, parallel group, multi-centre trial of carfilzomib, cyclophosphamide and dexamethasone (CCD) vs. cyclophosphamide, bortezomib (Velcade) and dexamethasone (CVD) for first relapse and primary refractory multiple myeloma.

PubMed

Brown, Sarah; Hinsley, Samantha; Ballesteros, Mónica; Bourne, Sue; McGarry, Paul; Sherratt, Debbie; Flanagan, Louise; Gregory, Walter; Cavenagh, Jamie; Owen, Roger; Williams, Cathy; Kaiser, Martin; Low, Eric; Yong, Kwee

2016-01-01

Multiple myeloma is a plasma cell tumour with an annual incidence in the UK of approximately 40-50 per million i.e. about 4500 new cases per annum. The triple combination cyclophosphamide, bortezomib (Velcade®) and dexamethasone (CVD) is an effective regimen at relapse and has emerged in recent years as the standard therapy at first relapse in the UK. Carfilzomib has good activity as a single agent in the relapsed setting, and it is expected that efficacy will be improved when used in combination with dexamethasone and cyclophosphamide. MUK Five is a phase II open label, randomised, controlled, parallel group, multi-centre trial that will compare the activity of carfilzomib, cyclophosphamide and dexamethasone (CCD) with that of CVD, given over an equivalent treatment period (24 weeks), in participants with multiple myeloma at first relapse, or refractory to no more than 1 line of treatment. In addition, the study also aims to assess the utility of a maintenance schedule of carfilzomib in these participants. The primary objective of the trial is to assess whether CCD provides non-inferior activity in terms of ≥ VGPR rates at 24 weeks, and whether the addition of maintenance treatment with carfilzomib to CCD provides superior activity in terms of progression-free survival, as compared to CCD with no maintenance. Secondary objectives include comparing toxicity profiles, further summarizing and comparing the activity of the different treatment arms and analysis of the effect of each treatment arm on minimal residual disease status. The development of carfilzomib offers the opportunity to further explore the anti-tumour efficacy of proteasome inhibition and, based on the available evidence, it is important and timely to obtain data on the activity, toxicity and tolerability of this drug. In contrast to ongoing phase III trials, this phase II trial has a unique subset of participants diagnosed with multiple myeloma at first relapse or refractory to no more than 1 line of treatment and will also evaluate the utility of maintenance with carfilzomib for up to 18 months and investigate minimal residual disease status to provide information on depth of response and the prognostic impact thereof. The trial is registered under ISRCTN17354232, December 2012.

Sensing Impacts of the Fate of Trace Explosives Signatures Under Environmental Conditions

DTIC Science & Technology

2010-01-01

vial with a pair of clean metal tweezers. A 10 mL aliquot of CHROMASOLV® Plus HPLC -grade acetone was dispensed on the wide surfaces of the sample...Evaporator Workstation under a nitrogen purge stream in a 50 ºC water bath and reconstituted with CHROMASOLV® HPLC -grade acetonitrile to 500 L... simultaneously on the two parallel GC columns, using a refrigerated (ɠ °C) 100-vial autosampler and two parallel auto-injectors. Column 1 (Restek 562719

Study of CCD Eyepiece on T-4 Theodolite.

DTIC Science & Technology

1982-11-01

11. REVIEW OF TWO-COLOR REFRACTOMETRY OBJECTIVES .....................3 III, ACHIEVEMENTS IN TWO- COLOR REFRACTOMETRY DURING THIS CONTRA...indirectly influence the questions of star catalogs. The initial efforts under this contract were addressed toward Two- Color Refractometry . This is a...in the astroposition procedure. Future efforts on other projects will consist of the return to the Two-Color Refractometry to provide a parallel

A dual communicator and dual grid-resolution algorithm for petascale simulations of turbulent mixing at high Schmidt number

NASA Astrophysics Data System (ADS)

Clay, M. P.; Buaria, D.; Gotoh, T.; Yeung, P. K.

2017-10-01

A new dual-communicator algorithm with very favorable performance characteristics has been developed for direct numerical simulation (DNS) of turbulent mixing of a passive scalar governed by an advection-diffusion equation. We focus on the regime of high Schmidt number (S c), where because of low molecular diffusivity the grid-resolution requirements for the scalar field are stricter than those for the velocity field by a factor √{ S c }. Computational throughput is improved by simulating the velocity field on a coarse grid of Nv3 points with a Fourier pseudo-spectral (FPS) method, while the passive scalar is simulated on a fine grid of Nθ3 points with a combined compact finite difference (CCD) scheme which computes first and second derivatives at eighth-order accuracy. A static three-dimensional domain decomposition and a parallel solution algorithm for the CCD scheme are used to avoid the heavy communication cost of memory transposes. A kernel is used to evaluate several approaches to optimize the performance of the CCD routines, which account for 60% of the overall simulation cost. On the petascale supercomputer Blue Waters at the University of Illinois, Urbana-Champaign, scalability is improved substantially with a hybrid MPI-OpenMP approach in which a dedicated thread per NUMA domain overlaps communication calls with computational tasks performed by a separate team of threads spawned using OpenMP nested parallelism. At a target production problem size of 81923 (0.5 trillion) grid points on 262,144 cores, CCD timings are reduced by 34% compared to a pure-MPI implementation. Timings for 163843 (4 trillion) grid points on 524,288 cores encouragingly maintain scalability greater than 90%, although the wall clock time is too high for production runs at this size. Performance monitoring with CrayPat for problem sizes up to 40963 shows that the CCD routines can achieve nearly 6% of the peak flop rate. The new DNS code is built upon two existing FPS and CCD codes. With the grid ratio Nθ /Nv = 8, the disparity in the computational requirements for the velocity and scalar problems is addressed by splitting the global communicator MPI_COMM_WORLD into disjoint communicators for the velocity and scalar fields, respectively. Inter-communicator transfer of the velocity field from the velocity communicator to the scalar communicator is handled with discrete send and non-blocking receive calls, which are overlapped with other operations on the scalar communicator. For production simulations at Nθ = 8192 and Nv = 1024 on 262,144 cores for the scalar field, the DNS code achieves 94% strong scaling relative to 65,536 cores and 92% weak scaling relative to Nθ = 1024 and Nv = 128 on 512 cores.

MAX-DOAS observations from ground, ship, and research aircraft: maximizing signal-to-noise to measure 'weak' absorbers

NASA Astrophysics Data System (ADS)

Volkamer, Rainer; Coburn, Sean; Dix, Barbara; Sinreich, Roman

2009-08-01

Multi AXis Differential Optical Absorption Spectroscopy (MAX-DOAS) instruments, as solar straylight satellites, require an accurate characterization and elimination of Fraunhofer lines from solar straylight spectra to measure the atmospheric column abundance of reactive gases that destroy toxic and heat trapping ozone and form climate cooling aerosols, like glyoxal (CHOCHO), iodine oxide (IO), or bromine oxide (BrO). The currently achievable noise levels with state-of-the-art DOAS instruments are limited to δ'DL ~ 10-4 (noise equivalent differential optical density, δ') further noise reductions are typically not straightforward, and the reason for this barrier is not well understood. Here we demonstrate that the nonlinearity of state-of-the-art CCD detectors poses a limitation to accurately characterize Fraunhofer lines; the incomplete elimination of Fraunhofer lines is found to cause residual structures of δ' ~ 10-4, and only partially accounted by fitting of an "offset" spectrum. We have developed a novel software tool, the CU Data Acquisition Code that overcomes this barrier by actively controlling the CCD saturation level, and demonstrates that δ'DL on the order of 10-5 are possible without apparent limitations from the presence of Fraunhofer lines. The software also implements active control of the elevation angle (angle with respect to the horizon) by means of a Motion Compensation System for use with mobile MAX-DOAS deployments from ships and aircraft. Finally, a novel approach to convert slant column densities into line-of-sight averaged concentrations is discussed.

Microchannel cross load array with dense parallel input

DOEpatents

Swierkowski, Stefan P.

2004-04-06

An architecture or layout for microchannel arrays using T or Cross (+) loading for electrophoresis or other injection and separation chemistry that are performed in microfluidic configurations. This architecture enables a very dense layout of arrays of functionally identical shaped channels and it also solves the problem of simultaneously enabling efficient parallel shapes and biasing of the input wells, waste wells, and bias wells at the input end of the separation columns. One T load architecture uses circular holes with common rows, but not columns, which allows the flow paths for each channel to be identical in shape, using multiple mirror image pieces. Another T load architecture enables the access hole array to be formed on a biaxial, collinear grid suitable for EDM micromachining (square holes), with common rows and columns.

Full-Physics Inverse Learning Machine for Satellite Remote Sensing of Ozone Profile Shapes and Tropospheric Columns

NASA Astrophysics Data System (ADS)

Xu, J.; Heue, K.-P.; Coldewey-Egbers, M.; Romahn, F.; Doicu, A.; Loyola, D.

2018-04-01

Characterizing vertical distributions of ozone from nadir-viewing satellite measurements is known to be challenging, particularly the ozone information in the troposphere. A novel retrieval algorithm called Full-Physics Inverse Learning Machine (FP-ILM), has been developed at DLR in order to estimate ozone profile shapes based on machine learning techniques. In contrast to traditional inversion methods, the FP-ILM algorithm formulates the profile shape retrieval as a classification problem. Its implementation comprises a training phase to derive an inverse function from synthetic measurements, and an operational phase in which the inverse function is applied to real measurements. This paper extends the ability of the FP-ILM retrieval to derive tropospheric ozone columns from GOME- 2 measurements. Results of total and tropical tropospheric ozone columns are compared with the ones using the official GOME Data Processing (GDP) product and the convective-cloud-differential (CCD) method, respectively. Furthermore, the FP-ILM framework will be used for the near-real-time processing of the new European Sentinel sensors with their unprecedented spectral and spatial resolution and corresponding large increases in the amount of data.

Improved accuracies for satellite tracking

NASA Technical Reports Server (NTRS)

Kammeyer, P. C.; Fiala, A. D.; Seidelmann, P. K.

1991-01-01

A charge coupled device (CCD) camera on an optical telescope which follows the stars can be used to provide high accuracy comparisons between the line of sight to a satellite, over a large range of satellite altitudes, and lines of sight to nearby stars. The CCD camera can be rotated so the motion of the satellite is down columns of the CCD chip, and charge can be moved from row to row of the chip at a rate which matches the motion of the optical image of the satellite across the chip. Measurement of satellite and star images, together with accurate timing of charge motion, provides accurate comparisons of lines of sight. Given lines of sight to stars near the satellite, the satellite line of sight may be determined. Initial experiments with this technique, using an 18 cm telescope, have produced TDRS-4 observations which have an rms error of 0.5 arc second, 100 m at synchronous altitude. Use of a mosaic of CCD chips, each having its own rate of charge motion, in the focal place of a telescope would allow point images of a geosynchronous satellite and of stars to be formed simultaneously in the same telescope. The line of sight of such a satellite could be measured relative to nearby star lines of sight with an accuracy of approximately 0.03 arc second. Development of a star catalog with 0.04 arc second rms accuracy and perhaps ten stars per square degree would allow determination of satellite lines of sight with 0.05 arc second rms absolute accuracy, corresponding to 10 m at synchronous altitude. Multiple station time transfers through a communications satellite can provide accurate distances from the satellite to the ground stations. Such observations can, if calibrated for delays, determine satellite orbits to an accuracy approaching 10 m rms.

Parallel array of independent thermostats for column separations

DOEpatents

Foret, Frantisek; Karger, Barry L.

2005-08-16

A thermostat array including an array of two or more capillary columns (10) or two or more channels in a microfabricated device is disclosed. A heat conductive material (12) surrounded each individual column or channel in array, each individual column or channel being thermally insulated from every other individual column or channel. One or more independently controlled heating or cooling elements (14) is positioned adjacent to individual columns or channels within the heat conductive material, each heating or cooling element being connected to a source of heating or cooling, and one or more independently controlled temperature sensing elements (16) is positioned adjacent to the individual columns or channels within the heat conductive material. Each temperature sensing element is connected to a temperature controller.

Photon counting image sensor development for astronomical applications

NASA Technical Reports Server (NTRS)

Jenkins, Edward B.

1987-01-01

Specially built intensified CCD (ICCD) detector tubes were purchased and the performance of the electron bombardment process was investigated. In addition to studying the signal characteristics of the photoevents, there was interest in demonstrating that back-illuminated chips were not susceptible to radiation damage to their clocking electrodes. How to perform a centroid analysis for a 2-dimensional Gaussian distribution of charge is described. Measurement of the projection (along columns or rows) of the average charge spread profile is discussed. The development and flight of the Interstellar Medium Absorption Profile Spectrograph (IMAPS) is discussed.

First qualification and selection of the eROSITA PNCCDs

NASA Astrophysics Data System (ADS)

Schächner, G.; Andritschke, R.; Hälker, O.; Herrmann, S.; Kimmel, N.; Meidinger, N.; Strüder, L.

2010-12-01

For the X-ray astronomy instrument eROSITA a framestore PNCCD was developed by the MPI Halbleiterlabor. The PNCCD has an image area of 384×384 pixels with a size of 75 μm×75 μm. Each channel of the PNCCD has an own readout anode which allows parallel amplification and signal processing of the CCD signals of one row. The first measurements for the spectroscopic characterization of the PNCCDs are made with a special measurement setup—the so-called Cold Chuck Probe Station. The Cold Chuck Probe Station allows to fully operate the CCD without mounting and bonding the chip on a PCB as the CCD is contacted only with needles. Thus all eROSITA PNCCDs can be qualified under the same measurement conditions and with an identical electronic setup. Therefore the results can be compared directly. The spectroscopic properties of the PNCCDs, like the charge transfer efficiency and the energy resolution are measured. Also pixel defects such as bright pixels or non-transferring pixels are detected. With the Cold Chuck Probe Station a readout noise of 2.7 e - ENC can be achieved and reliable measurement results obtained. Based on these results the best PNCCDs will be selected for eROSITA.

A multithreaded and GPU-optimized compact finite difference algorithm for turbulent mixing at high Schmidt number using petascale computing

NASA Astrophysics Data System (ADS)

Clay, M. P.; Yeung, P. K.; Buaria, D.; Gotoh, T.

2017-11-01

Turbulent mixing at high Schmidt number is a multiscale problem which places demanding requirements on direct numerical simulations to resolve fluctuations down the to Batchelor scale. We use a dual-grid, dual-scheme and dual-communicator approach where velocity and scalar fields are computed by separate groups of parallel processes, the latter using a combined compact finite difference (CCD) scheme on finer grid with a static 3-D domain decomposition free of the communication overhead of memory transposes. A high degree of scalability is achieved for a 81923 scalar field at Schmidt number 512 in turbulence with a modest inertial range, by overlapping communication with computation whenever possible. On the Cray XE6 partition of Blue Waters, use of a dedicated thread for communication combined with OpenMP locks and nested parallelism reduces CCD timings by 34% compared to an MPI baseline. The code has been further optimized for the 27-petaflops Cray XK7 machine Titan using GPUs as accelerators with the latest OpenMP 4.5 directives, giving 2.7X speedup compared to CPU-only execution at the largest problem size. Supported by NSF Grant ACI-1036170, the NCSA Blue Waters Project with subaward via UIUC, and a DOE INCITE allocation at ORNL.

An approach to enhance pnetCDF performance in ...

EPA Pesticide Factsheets

Data intensive simulations are often limited by their I/O (input/output) performance, and "novel" techniques need to be developed in order to overcome this limitation. The software package pnetCDF (parallel network Common Data Form), which works with parallel file systems, was developed to address this issue by providing parallel I/O capability. This study examines the performance of an application-level data aggregation approach which performs data aggregation along either row or column dimension of MPI (Message Passing Interface) processes on a spatially decomposed domain, and then applies the pnetCDF parallel I/O paradigm. The test was done with three different domain sizes which represent small, moderately large, and large data domains, using a small-scale Community Multiscale Air Quality model (CMAQ) mock-up code. The examination includes comparing I/O performance with traditional serial I/O technique, straight application of pnetCDF, and the data aggregation along row and column dimension before applying pnetCDF. After the comparison, "optimal" I/O configurations of this application-level data aggregation approach were quantified. Data aggregation along the row dimension (pnetCDFcr) works better than along the column dimension (pnetCDFcc) although it may perform slightly worse than the straight pnetCDF method with a small number of processors. When the number of processors becomes larger, pnetCDFcr outperforms pnetCDF significantly. If the number of proces

Extended surface parallel coating inspection method

DOEpatents

Naulleau, Patrick P.

2006-03-21

Techniques for rapidly characterizing reflective surfaces and especially multi-layer EUV reflective surfaces of optical components involve illuminating the entire reflective surface instantaneously and detecting the image far field. The technique provides a mapping of points on the reflective surface to corresponding points on a detector, e.g., CCD. This obviates the need to scan a probe over the entire surface of the optical component. The reflective surface can be flat, convex, or concave.

High speed infrared imaging system and method

DOEpatents

Zehnder, Alan T.; Rosakis, Ares J.; Ravichandran, G.

2001-01-01

A system and method for radiation detection with an increased frame rate. A semi-parallel processing configuration is used to process a row or column of pixels in a focal-plane array in parallel to achieve a processing rate up to and greater than 1 million frames per second.

Parallel-quadrature phase-shifting digital holographic microscopy using polarization beam splitter

PubMed Central

Das, Bhargab; Yelleswarapu, Chandra S; Rao, DVGLN

2012-01-01

We present a digital holography microscopy technique based on parallel-quadrature phase-shifting method. Two π/2 phase-shifted holograms are recorded simultaneously using polarization phase-shifting principle, slightly off-axis recording geometry, and two identical CCD sensors. The parallel phase-shifting is realized by combining circularly polarized object beam with a 45° degree polarized reference beam through a polarizing beam splitter. DC term is eliminated by subtracting the two holograms from each other and the object information is reconstructed after selecting the frequency spectrum of the real image. Both amplitude and phase object reconstruction results are presented. Simultaneous recording eliminates phase errors caused by mechanical vibrations and air turbulences. The slightly off-axis recording geometry with phase-shifting allows a much larger dimension of the spatial filter for reconstruction of the object information. This leads to better reconstruction capability than traditional off-axis holography. PMID:23109732

Supercritical Fluid Chromatography of Drugs: Parallel Factor Analysis for Column Testing in a Wide Range of Operational Conditions

PubMed Central

Al-Degs, Yahya; Andri, Bertyl; Thiébaut, Didier; Vial, Jérôme

2017-01-01

Retention mechanisms involved in supercritical fluid chromatography (SFC) are influenced by interdependent parameters (temperature, pressure, chemistry of the mobile phase, and nature of the stationary phase), a complexity which makes the selection of a proper stationary phase for a given separation a challenging step. For the first time in SFC studies, Parallel Factor Analysis (PARAFAC) was employed to evaluate the chromatographic behavior of eight different stationary phases in a wide range of chromatographic conditions (temperature, pressure, and gradient elution composition). Design of Experiment was used to optimize experiments involving 14 pharmaceutical compounds present in biological and/or environmental samples and with dissimilar physicochemical properties. The results showed the superiority of PARAFAC for the analysis of the three-way (column × drug × condition) data array over unfolding the multiway array to matrices and performing several classical principal component analyses. Thanks to the PARAFAC components, similarity in columns' function, chromatographic trend of drugs, and correlation between separation conditions could be simply depicted: columns were grouped according to their H-bonding forces, while gradient composition was dominating for condition classification. Also, the number of drugs could be efficiently reduced for columns classification as some of them exhibited a similar behavior, as shown by hierarchical clustering based on PARAFAC components. PMID:28695040

The relation between the column density structures and the magnetic field orientation in the Vela C molecular complex

NASA Astrophysics Data System (ADS)

Soler, J. D.; Ade, P. A. R.; Angilè, F. E.; Ashton, P.; Benton, S. J.; Devlin, M. J.; Dober, B.; Fissel, L. M.; Fukui, Y.; Galitzki, N.; Gandilo, N. N.; Hennebelle, P.; Klein, J.; Li, Z.-Y.; Korotkov, A. L.; Martin, P. G.; Matthews, T. G.; Moncelsi, L.; Netterfield, C. B.; Novak, G.; Pascale, E.; Poidevin, F.; Santos, F. P.; Savini, G.; Scott, D.; Shariff, J. A.; Thomas, N. E.; Tucker, C. E.; Tucker, G. S.; Ward-Thompson, D.

2017-07-01

We statistically evaluated the relative orientation between gas column density structures, inferred from Herschel submillimetre observations, and the magnetic field projected on the plane of sky, inferred from polarized thermal emission of Galactic dust observed by the Balloon-borne Large-Aperture Submillimetre Telescope for Polarimetry (BLASTPol) at 250, 350, and 500 μm, towards the Vela C molecular complex. First, we find very good agreement between the polarization orientations in the three wavelength-bands, suggesting that, at the considered common angular resolution of 3.´0 that corresponds to a physical scale of approximately 0.61 pc, the inferred magnetic field orientation is not significantly affected by temperature or dust grain alignment effects. Second, we find that the relative orientation between gas column density structures and the magnetic field changes progressively with increasing gas column density, from mostly parallel or having no preferred orientation at low column densities to mostly perpendicular at the highest column densities. This observation is in agreement with previous studies by the Planck collaboration towards more nearby molecular clouds. Finally, we find a correspondencebetween (a) the trends in relative orientation between the column density structures and the projected magnetic field; and (b) the shape of the column density probability distribution functions (PDFs). In the sub-regions of Vela C dominated by one clear filamentary structure, or "ridges", where the high-column density tails of the PDFs are flatter, we find a sharp transition from preferentially parallel or having no preferred relative orientation at low column densities to preferentially perpendicular at highest column densities. In the sub-regions of Vela C dominated by several filamentary structures with multiple orientations, or "nests", where the maximum values of the column density are smaller than in the ridge-like sub-regions and the high-column density tails of the PDFs are steeper, such a transition is also present, but it is clearly less sharp than in the ridge-like sub-regions. Both of these results suggest that the magnetic field is dynamically important for the formation of density structures in this region.

Performance of an extended dynamic range time delay integration charge coupled device (XDR TDI CCD) for high-intrascene dynamic range scanning

NASA Astrophysics Data System (ADS)

Levine, Peter A.; Dawson, Robin M.; Andrews, James T.; Bhaskaran, Mahalingham; Furst, David; Hsueh, Fu-Lung; Meray, Grazyna M.; Sudol, Thomas M.; Swain, Pradyumna K.; Tower, John R.

2003-05-01

Many applications, such as industrial inspection and overhead reconnaissance benefit from line scanning architectures where time delay integration (TDI) significantly improves sensitivity. CCDs are particularly well suited to the TDI architecture since charge is transferred virtually noiselessly down the column. Sarnoff's TDI CCDs have demonstrated extremely high speeds where a 7200 x 64, 8 um pixel device with 120 output ports demonstrated a vertical line transfer rate greater than 800 kHz. The most recent addition to Sarnoff's TDI technology is the implementation of extended dynamic range (XDR) in high speed, back illuminated TDI CCDs. The optical, intrascene dynamic range can be adjusted in the design of the imager with measured dynamic ranges exceeding 2,000,000:1 with no degradation in low light performance. The device provides a piecewise linear response to light where multiple slopes and break points can be set during the CCD design. A description of the device architecture and measured results from fabricated XDR TDI CCDs are presented.

Hyper Suprime-Cam: characteristics of 116 fully depleted back-illuminated CCDs

NASA Astrophysics Data System (ADS)

Kamata, Yukiko; Miyazaki, Satoshi; Nakaya, Hidehiko; Komiyama, Yutaka; Obuchi, Yoshiyuki; Kawanomoto, Satoshi; Uraguchi, Fumihiro; Utsumi, Yosuke; Suzuki, Hisanori; Miyazaki, Yasuhito; Muramatsu, Masaharu

2012-07-01

Hyper Suprime-Cam (HSC)1,2 is a wide field imaging camera with the field of view (FOV) 1.5 degree diameter, which is to be installed at the prime focus of the Subaru Telescope. The large FOV is realized by the 116 2K × 4K pixels fully depleted back-illuminated CCD (FDCCD) with 15 μm pixel square. The acceptance inspection of the CCDs started around the end of 2009 and finished June 2011. We measured basic characteristics such as charge transfer efficiency (CTE), dark current, readout noise, linearity and the number of the dead column for all CCDs, and measured the quantum effciency (QE) of 21 CCDs. As a result, we confirmed exceptional quality and performance fdor all CCDs ans were able to select the best pissible 116 CCDs. We also measured the flatness of each CCD at room temperature, and optimally placed them on the focal plane plate. In this paper, we report the results of the acceptance inspection asn the installation process into the HSC dewar3,4.

The fast and accurate 3D-face scanning technology based on laser triangle sensors

NASA Astrophysics Data System (ADS)

Wang, Jinjiang; Chang, Tianyu; Ge, Baozhen; Tian, Qingguo; Chen, Yang; Kong, Bin

2013-08-01

A laser triangle scanning method and the structure of 3D-face measurement system were introduced. In presented system, a liner laser source was selected as an optical indicated signal in order to scanning a line one times. The CCD image sensor was used to capture image of the laser line modulated by human face. The system parameters were obtained by system calibrated calculated. The lens parameters of image part of were calibrated with machine visual image method and the triangle structure parameters were calibrated with fine wire paralleled arranged. The CCD image part and line laser indicator were set with a linear motor carry which can achieve the line laser scanning form top of the head to neck. For the nose is ledge part and the eyes are sunk part, one CCD image sensor can not obtain the completed image of laser line. In this system, two CCD image sensors were set symmetric at two sides of the laser indicator. In fact, this structure includes two laser triangle measure units. Another novel design is there laser indicators were arranged in order to reduce the scanning time for it is difficult for human to keep static for longer time. The 3D data were calculated after scanning. And further data processing include 3D coordinate refine, mesh calculate and surface show. Experiments show that this system has simply structure, high scanning speed and accurate. The scanning range covers the whole head of adult, the typical resolution is 0.5mm.

Nonlinear wave interaction in a plasma column

NASA Technical Reports Server (NTRS)

Larsen, J.

1972-01-01

Two particular cases of nonlinear wave interaction in a plasma column were investigated. The frequencies of the waves were on the order of magnitude of the electron plasma frequency, and ion motion was neglected. The nonlinear coupling of slow waves on a plasma column was studied by means of cold plasma theory, and the case of a plasma column surrounded by an infinite dielectric in the absence of a magnetic field was also examined. Nonlinear scattering from a plasma column in an electromagnetic field having it's magnetic field parallel to the axis of the column was investigated. Some experimental results on mode conversion in the presence of loss are presented along with some observations of nonlinear scattering, The effect of the earth's magnetic field and of discharge symmetry on the radiation pattern are discussed.

Programming a hillslope water movement model on the MPP

NASA Technical Reports Server (NTRS)

Devaney, J. E.; Irving, A. R.; Camillo, P. J.; Gurney, R. J.

1987-01-01

A physically based numerical model was developed of heat and moisture flow within a hillslope on a parallel architecture computer, as a precursor to a model of a complete catchment. Moisture flow within a catchment includes evaporation, overland flow, flow in unsaturated soil, and flow in saturated soil. Because of the empirical evidence that moisture flow in unsaturated soil is mainly in the vertical direction, flow in the unsaturated zone can be modeled as a series of one dimensional columns. This initial version of the hillslope model includes evaporation and a single column of one dimensional unsaturated zone flow. This case has already been solved on an IBM 3081 computer and is now being applied to the massively parallel processor architecture so as to make the extension to the one dimensional case easier and to check the problems and benefits of using a parallel architecture machine.

Report on the eROSITA camera system

NASA Astrophysics Data System (ADS)

Meidinger, Norbert; Andritschke, Robert; Bornemann, Walter; Coutinho, Diogo; Emberger, Valentin; Hälker, Olaf; Kink, Walter; Mican, Benjamin; Müller, Siegfried; Pietschner, Daniel; Predehl, Peter; Reiffers, Jonas

2014-07-01

The eROSITA space telescope is currently developed for the determination of cosmological parameters and the equation of state of dark energy via evolution of clusters of galaxies. Furthermore, the instrument development was strongly motivated by the intention of a first imaging X-ray all-sky survey enabling measurements above 2 keV. eROSITA is a scientific payload on the Russian research satellite SRG. Its destination after launch is the Lagrangian point L2. The observational program of the observatory divides into an all-sky survey and pointed observations and takes in total about 7.5 years. The instrument comprises an array of 7 identical and parallel aligned telescopes. Each of the seven focal plane cameras is equipped with a PNCCD detector, an enhanced type of the XMM-Newton focal plane detector. This instrumentation permits spectroscopy and imaging of X-rays in the energy band from 0.3 keV to 10 keV with a field of view of 1.0 degree. The camera development is done at the Max-Planck-Institute for extraterrestrial physics. Key component of each camera is the PNCCD chip. This silicon sensor is a back-illuminated, fully depleted and column-parallel type of charge coupled device. The image area of the 450 micron thick frame-transfer CCD comprises an array of 384 x 384 pixels, each with a size of 75 micron x 75 micron. Readout of the signal charge that is generated by an incident X-ray photon in the CCD is accomplished by an ASIC, the so-called eROSITA CAMEX. It provides 128 parallel analog signal processing channels but multiplexes the signals finally to one output which feeds the detector signals to a fast 14-bit ADC. The read noise of this system is equivalent to a noise charge of about 2.5 electrons rms. We achieve an energy resolution close to the theoretical limit given by Fano noise (except for very low energies). For example, the FWHM at an energy of 5.9 keV is approximately 140 eV. The complete camera assembly comprises the camera head with the detector as key component, the electronics for detector operation as well as data acquisition and the filter wheel unit. In addition to the on-chip light blocking filter directly deposited on the photon entrance window of the PNCCD, an external filter can be moved in front of the sensor, which serves also for contamination protection. Furthermore, an on-board calibration source emitting several fluorescence lines is accommodated on the filter wheel mechanism for the purpose of in-orbit calibration. Since the spectroscopic silicon sensors need cooling down to -95°C to mitigate best radiation damage effects, an elaborate cooling system is necessary. It consists of two different types of heat pipes linking the seven detectors to two radiators. Based on the tests with an engineering model, a flight design was developed for the camera and a qualification model has been built. The tests and the performance of this camera is presented in the following. In conclusion an outlook on the flight cameras is given.

Smart photodetector arrays for error control in page-oriented optical memory

NASA Astrophysics Data System (ADS)

Schaffer, Maureen Elizabeth

1998-12-01

Page-oriented optical memories (POMs) have been proposed to meet high speed, high capacity storage requirements for input/output intensive computer applications. This technology offers the capability for storage and retrieval of optical data in two-dimensional pages resulting in high throughput data rates. Since currently measured raw bit error rates for these systems fall several orders of magnitude short of industry requirements for binary data storage, powerful error control codes must be adopted. These codes must be designed to take advantage of the two-dimensional memory output. In addition, POMs require an optoelectronic interface to transfer the optical data pages to one or more electronic host systems. Conventional charge coupled device (CCD) arrays can receive optical data in parallel, but the relatively slow serial electronic output of these devices creates a system bottleneck thereby eliminating the POM advantage of high transfer rates. Also, CCD arrays are "unintelligent" interfaces in that they offer little data processing capabilities. The optical data page can be received by two-dimensional arrays of "smart" photo-detector elements that replace conventional CCD arrays. These smart photodetector arrays (SPAs) can perform fast parallel data decoding and error control, thereby providing an efficient optoelectronic interface between the memory and the electronic computer. This approach optimizes the computer memory system by combining the massive parallelism and high speed of optics with the diverse functionality, low cost, and local interconnection efficiency of electronics. In this dissertation we examine the design of smart photodetector arrays for use as the optoelectronic interface for page-oriented optical memory. We review options and technologies for SPA fabrication, develop SPA requirements, and determine SPA scalability constraints with respect to pixel complexity, electrical power dissipation, and optical power limits. Next, we examine data modulation and error correction coding for the purpose of error control in the POM system. These techniques are adapted, where possible, for 2D data and evaluated as to their suitability for a SPA implementation in terms of BER, code rate, decoder time and pixel complexity. Our analysis shows that differential data modulation combined with relatively simple block codes known as array codes provide a powerful means to achieve the desired data transfer rates while reducing error rates to industry requirements. Finally, we demonstrate the first smart photodetector array designed to perform parallel error correction on an entire page of data and satisfy the sustained data rates of page-oriented optical memories. Our implementation integrates a monolithic PN photodiode array and differential input receiver for optoelectronic signal conversion with a cluster error correction code using 0.35-mum CMOS. This approach provides high sensitivity, low electrical power dissipation, and fast parallel correction of 2 x 2-bit cluster errors in an 8 x 8 bit code block to achieve corrected output data rates scalable to 102 Gbps in the current technology increasing to 1.88 Tbps in 0.1-mum CMOS.

Signal processing method of the diameter measurement system based on CCD parallel light projection method

NASA Astrophysics Data System (ADS)

Song, Qing; Zhu, Sijia; Yan, Han; Wu, Wenqian

2008-03-01

Parallel light projection method for the diameter measurement is to project the workpiece to be measured on the photosensitive units of CCD, but the original signal output from CCD cannot be directly used for counting or measurement. The weak signal with high-frequency noise should be filtered and amplified firstly. This paper introduces RC low-pass filter and multiple feed-back second-order low-pass filter with infinite gain. Additionally there is always dispersion on the light band and the output signal has a transition between the irradiant area and the shadow, because of the instability of the light source intensity and the imperfection of the light system adjustment. To obtain exactly the shadow size related to the workpiece diameter, binary-value processing is necessary to achieve a square wave. Comparison method and differential method can be adopted for binary-value processing. There are two ways to decide the threshold value when using voltage comparator: the fixed level method and the floated level method. The latter has a high accuracy. Deferential method is to output two spike pulses with opposite pole by the rising edge and the failing edge of the video signal related to the differential circuit firstly, then the rising edge of the signal output from the differential circuit is acquired by half-wave rectifying circuit. After traveling through the zero passing comparator and the maintain- resistance edge trigger, the square wave which indicates the measured size is acquired at last. And then it is used for filling through standard pulses and for counting through the counter. Data acquisition and information processing is accomplished by the computer and the control software. This paper will introduce in detail the design and analysis of the filter circuit, binary-value processing circuit and the interface circuit towards the computer.

Image sensor with high dynamic range linear output

NASA Technical Reports Server (NTRS)

Yadid-Pecht, Orly (Inventor); Fossum, Eric R. (Inventor)

2007-01-01

Designs and operational methods to increase the dynamic range of image sensors and APS devices in particular by achieving more than one integration times for each pixel thereof. An APS system with more than one column-parallel signal chains for readout are described for maintaining a high frame rate in readout. Each active pixel is sampled for multiple times during a single frame readout, thus resulting in multiple integration times. The operation methods can also be used to obtain multiple integration times for each pixel with an APS design having a single column-parallel signal chain for readout. Furthermore, analog-to-digital conversion of high speed and high resolution can be implemented.

Long-range attraction of an ultrarelativistic electron beam by a column of neutral plasma

NASA Astrophysics Data System (ADS)

Adli, E.; Lindstrøm, C. A.; Allen, J.; Clarke, C. I.; Frederico, J.; Gessner, S. J.; Green, S. Z.; Hogan, M. J.; Litos, M. D.; O'Shea, B.; Yakimenko, V.; An, W.; Clayton, C. E.; Marsh, K. A.; Mori, W. B.; Joshi, C.; Vafaei-Najafabadi, N.; Corde, S.; Lu, W.

2016-10-01

We report on the experimental observation of the attraction of a beam of ultrarelativistic electrons towards a column of neutral plasma. In experiments performed at the FACET test facility at SLAC we observe that an electron beam moving parallel to a neutral plasma column, at an initial distance of many plasma column radii, is attracted into the column. Once the beam enters the plasma it drives a plasma wake similar to that of an electron beam entering the plasma column head-on. A simple analytical model is developed in order to capture the essential physics of the attractive force. The attraction is further studied by 3D particle-in-cell numerical simulations. The results are an important step towards better understanding of particle beam-plasma interactions in general and plasma wakefield accelerator technology in particular.

Multiannual tropical tropospheric ozone columns and the case of the 2015 el Niño event

NASA Astrophysics Data System (ADS)

Leventidou, Elpida; Eichmann, Kai-Uwe; Weber, Mark; Burrows, John P.

2016-04-01

Stratospheric ozone is well known for protecting the surface from harmful ultraviolet solar radiation whereas ozone in the troposphere plays a more complex role. In the lower troposphere ozone can be extremely harmful for human health as it can oxidize biological tissues and causes respiratory problems. Several studies have shown that the tropospheric ozone burden (300±30Tg (IPCC, 2007)) increases by 1-7% per decade in the tropics (Beig and Singh, 2007; Cooper et al., 2014) which makes the need to monitor it on a global scale crucial. Remote sensing from satellites has been proven to be very useful in providing consistent information of tropospheric ozone concentrations over large areas. Tropical tropospheric ozone columns can be retrieved with the Convective Cloud Differential (CCD) technique (Ziemke et al. 1998) using retrieved total ozone columns and cloud parameters from space-borne observations. We have developed a CCD-IUP algorithm which was applied to GOME/ ERS-2 (1995-2003), SCIAMACHY/ Envisat (2002-2012), and GOME-2/ MetOpA (2007-2012) weighting function DOAS (Coldewey-Egbers et al., 2005, Weber et al., 2005) total ozone data. A unique long-term record of monthly averaged tropical tropospheric ozone columns (20°S - 20°N) was created starting in 1996. This dataset has been extensively validated by comparisons with SHADOZ (Thompson et al., 2003) ozonesonde data and limb-nadir Matching (Ebojie et al. 2014) tropospheric ozone data. The comparison shows good agreement with respect to range, inter-annual variation, and variance. Biases where found to be within 5DU and the RMS errors less than 10 DU. This 17-years dataset has been harmonized into one consistent time series, taking into account the three instruments' difference in ground pixel size. The harmonised dataset is used to determine tropical tropospheric ozone trends and climatological values. The 2015 el Niño event has been characterised as one of the top three strongest el Niños since 1950. El Niño events are major sources of the tropospheric ozone variability (Ziemke and Chandra,2003) due to changes in the convection pattern and large-scale circulation in the tropical Pacific region. More clouds and rainfall appear in the central and/or eastern Pacific whereas more dryness over Indonesia and as a result strongest forest fires. These effects cause enhanced tropospheric ozone columns over the Indonesian region and reduced over the eastern Pacific. The focus of this work is to present the first results of tropospheric ozone trends the last 17 years as long as to understand and quantify the tropical tropospheric ozone (TTCO) anomalies due to the 2015 el Niño event.

Motion systems providing three or four degrees of freedom

NASA Technical Reports Server (NTRS)

Chou, Richard C. (Inventor)

1982-01-01

A motion system is provided by a platform generally parallel to a base and connected thereto by a column and powered and controlled extensible members, at least three of which are connected between distributed points around the column. In a three degree of freedom device, the column is conical, rigidly supported at its base with a universal joint at its top. The points of attachment define triangles in the base and in the platform surrounding the column with one extensible member connected between each. In the four degree of freedom version, the column is modified by making it effectively a column which is pivoted or guided at the base or contains an extensible member, preferably retains its triangular shape and its universal joint connection to the platform at its apex. For stability four powered and controlled extensible members are provided between points in the base and platform distributed around the column, a preferred pattern of arrangement being a square with the column at the center.

Recent improvements of the JET lithium beam diagnostica)

NASA Astrophysics Data System (ADS)

Brix, M.; Dodt, D.; Dunai, D.; Lupelli, I.; Marsen, S.; Melson, T. F.; Meszaros, B.; Morgan, P.; Petravich, G.; Refy, D. I.; Silva, C.; Stamp, M.; Szabolics, T.; Zastrow, K.-D.; Zoletnik, S.; JET-EFDA Contributors

2012-10-01

A 60 kV neutral lithium diagnostic beam probes the edge plasma of JET for the measurement of electron density profiles. This paper describes recent enhancements of the diagnostic setup, new procedures for calibration and protection measures for the lithium ion gun during massive gas puffs for disruption mitigation. New light splitting optics allow in parallel beam emission measurements with a new double entrance slit CCD spectrometer (spectrally resolved) and a new interference filter avalanche photodiode camera (fast density and fluctuation studies).

Integrated Optical Synthetic Aperture Radar Processor.

DTIC Science & Technology

1987-09-01

acoustooptic cell was employed to input each radar return into a time-and-space integrating optical architecture comprised of several lenses, a CCD area array...acoustooptic cell and parallel rib waveguide structure. During the course of the literature survey, we became aware of an elegant and poten- tially profound...wave.) scatterer at (f , A(t) is the far-field pattern of the antenna. From the geometry of Si. 1. R can be written as [I-2R,/c - nT1 r(t) = A(nT) rectj

DOE Office of Scientific and Technical Information (OSTI.GOV)

Busbey, A.B.

Seismic Processing Workshop, a program by Parallel Geosciences of Austin, TX, is discussed in this column. The program is a high-speed, interactive seismic processing and computer analysis system for the Apple Macintosh II family of computers. Also reviewed in this column are three products from Wilkerson Associates of Champaign, IL. SubSide is an interactive program for basin subsidence analysis; MacFault and MacThrustRamp are programs for modeling faults.

A Novel Crosstalk Suppression Method of the 2-D Networked Resistive Sensor Array

PubMed Central

Wu, Jianfeng; Wang, Lei; Li, Jianqing; Song, Aiguo

2014-01-01

The 2-D resistive sensor array in the row–column fashion suffered from the crosstalk problem for parasitic parallel paths. Firstly, we proposed an Improved Isolated Drive Feedback Circuit with Compensation (IIDFCC) based on the voltage feedback method to suppress the crosstalk. In this method, a compensated resistor was specially used to reduce the crosstalk caused by the column multiplexer resistors and the adjacent row elements. Then, a mathematical equivalent resistance expression of the element being tested (EBT) of this circuit was analytically derived and verified by the circuit simulations. The simulation results show that the measurement method can greatly reduce the influence on the EBT caused by parasitic parallel paths for the multiplexers' channel resistor and the adjacent elements. PMID:25046011

Long-range attraction of an ultrarelativistic electron beam by a column of neutral plasma

DOE PAGES

Adli, Erik; Lindstrom, C. A.; Allen, J.; ...

2016-10-12

Here, we report on the experimental observation of the attraction of a beam of ultrarelativistic electrons towards a column of neutral plasma. In experiments performed at the FACET test facility at SLAC we observe that an electron beam moving parallel to a neutral plasma column, at an initial distance of many plasma column radii, is attracted into the column. Once the beam enters the plasma it drives a plasma wake similar to that of an electron beam entering the plasma column head-on. A simple analytical model is developed in order to capture the essential physics of the attractive force. Themore » attraction is further studied by 3D particle-in-cell numerical simulations. The results are an important step towards better understanding of particle beam–plasma interactions in general and plasma wakefield accelerator technology in particular.« less

Long-range attraction of an ultrarelativistic electron beam by a column of neutral plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adli, Erik; Lindstrom, C. A.; Allen, J.

Here, we report on the experimental observation of the attraction of a beam of ultrarelativistic electrons towards a column of neutral plasma. In experiments performed at the FACET test facility at SLAC we observe that an electron beam moving parallel to a neutral plasma column, at an initial distance of many plasma column radii, is attracted into the column. Once the beam enters the plasma it drives a plasma wake similar to that of an electron beam entering the plasma column head-on. A simple analytical model is developed in order to capture the essential physics of the attractive force. Themore » attraction is further studied by 3D particle-in-cell numerical simulations. The results are an important step towards better understanding of particle beam–plasma interactions in general and plasma wakefield accelerator technology in particular.« less

Imaging the Moon II: Webcam CCD Observations and Analysis (a Two-Week Lab for Non-Majors)

NASA Astrophysics Data System (ADS)

Sato, T.

2014-07-01

Imaging the Moon is a successful two-week lab involving real sky observations of the Moon in which students make telescopic observations and analyze their own images. Originally developed around the 35 mm film camera, a common household object adapted for astronomical work, the lab now uses webcams as film photography has evolved into an obscure specialty technology and increasing numbers of students have little familiarity with it. The printed circuit board with the CCD is harvested from a commercial webcam and affixed to a tube to mount on a telescope in place of an eyepiece. Image frames are compiled to form a lunar mosaic, and crater sizes are measured. Students also work through the logistical steps of telescope time assignment and scheduling. They learn to keep a schedule and work with uncertainties of weather in ways paralleling research observations. Because there is no need for a campus observatory, this lab can be replicated at a wide variety of institutions.

A new clocking method for a charge coupled device

DOE Office of Scientific and Technical Information (OSTI.GOV)

Umezu, Rika; Kitamoto, Shunji, E-mail: kitamoto@rikkyo.ac.jp; Murakami, Hiroshi

2014-07-15

We propose and demonstrate a new clocking method for a charge-coupled device (CCD). When a CCD is used for a photon counting detector of X-rays, its weak point is a limitation of its counting rate, because high counting rate makes non-negligible pile-up of photons. In astronomical usage, this pile-up is especially severe for an observation of a bright point-like object. One typical idea to reduce the pile-up is a parallel sum (P-sum) mode. This mode completely loses one-dimensional information. Our new clocking method, panning mode, provides complementary properties between the normal mode and the P-sum mode. We performed a simplemore » simulation in order to investigate a pile-up probability and compared the simulated result and actual obtained event rates. Using this simulation and the experimental results, we compared the pile-up tolerance of various clocking modes including our new method and also compared their other characteristics.« less

Science from Kepler Collateral Data: 50 Kilosecond per Year from 13 Million Star?

NASA Technical Reports Server (NTRS)

Kolodziejczak, J. J.; Caldwell, D. A.

2011-01-01

As each Kepler frame is read out, light from each star in a CCD column accumulates in successive pixels as they wait for the next row to be read out. This accumulation is the same in the masked rows at the start of the readout and virtual rows at the end of the readout as it is in the science data. A range of these "smear" rows are added together for each long cadence and sent to the ground for calibration purposes. We will introduce and describe this smear collateral data, discuss and demonstrate its potential use for scientific studies exclusive of Kepler calibration,.

Gas chromatography fractionation platform featuring parallel flame-ionization detection and continuous high-resolution analyte collection in 384-well plates.

PubMed

Jonker, Willem; Clarijs, Bas; de Witte, Susannah L; van Velzen, Martin; de Koning, Sjaak; Schaap, Jaap; Somsen, Govert W; Kool, Jeroen

2016-09-02

Gas chromatography (GC) is a superior separation technique for many compounds. However, fractionation of a GC eluate for analyte isolation and/or post-column off-line analysis is not straightforward, and existing platforms are limited in the number of fractions that can be collected. Moreover, aerosol formation may cause serious analyte losses. Previously, our group has developed a platform that resolved these limitations of GC fractionation by post-column infusion of a trap solvent prior to continuous small-volume fraction collection in a 96-wells plate (Pieke et al., 2013 [17]). Still, this GC fractionation set-up lacked a chemical detector for the on-line recording of chromatograms, and the introduction of trap solvent resulted in extensive peak broadening for late-eluting compounds. This paper reports advancements to the fractionation platform allowing flame ionization detection (FID) parallel to high-resolution collection of a full GC chromatograms in up to 384 nanofractions of 7s each. To this end, a post-column split was incorporated which directs part of the eluate towards FID. Furthermore, a solvent heating device was developed for stable delivery of preheated/vaporized trap solvent, which significantly reduced band broadening by post-column infusion. In order to achieve optimal analyte trapping, several solvents were tested at different flow rates. The repeatability of the optimized GC fraction collection process was assessed demonstrating the possibility of up-concentration of isolated analytes by repetitive analyses of the same sample. The feasibility of the improved GC fractionation platform for bioactivity screening of toxic compounds was studied by the analysis of a mixture of test pesticides, which after fractionation were subjected to a post-column acetylcholinesterase (AChE) assay. Fractions showing AChE inhibition could be unambiguously correlated with peaks from the parallel-recorded FID chromatogram. Copyright © 2016 Elsevier B.V. All rights reserved.

Characterisation of Crevice and Pit Solution Chemistries Using Capillary Electrophoresis with Contactless Conductivity Detector

PubMed Central

Nie, Mengyan; Wharton, Julian A.; Cranny, Andy; Harris, Nick R.; Wood, Robert J.K.; Stokes, Keith R.

2013-01-01

The ability to predict structural degradation in-service is often limited by a lack of understanding of the evolving chemical species occurring within a range of different microenvironments associated with corrosion sites. Capillary electrophoresis (CE) is capable of analysing nanolitre solution volumes with widely disparate concentrations of ionic species, thereby producing accurate and reliable results for the analysis of the chemical compositions found within microenvironment corrosion solutions, such as those found at crevice and pit corrosion sites. In this study, CE with contactless conductivity detection (CCD) has been used to characterize pitting and crevice corrosion solution chemistries for the first time. By using the capillary electrophoresis with contactless conductivity detection (CE-CCD) system, direct and simultaneous detection of seven metal cations (Cu2+, Ni2+, Fe3+, Fe2+, Cr3+, Mn2+, and Al3+) and chloride anions was achieved with a buffer solution of 10 mM 2,6-pyridinedicarboxylic acid and 0.5 mM cetyltrimethylammonium hydroxide at pH 4 using a pre-column complexation method. The detection limits obtained for the metal cations and chloride anions were 100 and 10 ppb, respectively. The CE-CCD methodology has been demonstrated to be a versatile technique capable of speciation and quantifying the ionic species generated within artificial pit (a pencil electrode) and crevice corrosion geometries for carbon steels and nickel-aluminium bronze, thus allowing the evolution of the solution chemistry to be assessed with time and the identification of the key corrosion analyte targets for structural health monitoring. PMID:28788335

Development of a driving method suitable for ultrahigh-speed shooting in a 2M-fps 300k-pixel single-chip color camera

NASA Astrophysics Data System (ADS)

Yonai, J.; Arai, T.; Hayashida, T.; Ohtake, H.; Namiki, J.; Yoshida, T.; Etoh, T. Goji

2012-03-01

We have developed an ultrahigh-speed CCD camera that can capture instantaneous phenomena not visible to the human eye and impossible to capture with a regular video camera. The ultrahigh-speed CCD was specially constructed so that the CCD memory between the photodiode and the vertical transfer path of each pixel can store 144 frames each. For every one-frame shot, the electric charges generated from the photodiodes are transferred in one step to the memory of all the parallel pixels, making ultrahigh-speed shooting possible. Earlier, we experimentally manufactured a 1M-fps ultrahigh-speed camera and tested it for broadcasting applications. Through those tests, we learned that there are cases that require shooting speeds (frame rate) of more than 1M fps; hence we aimed to develop a new ultrahigh-speed camera that will enable much faster shooting speeds than what is currently possible. Since shooting at speeds of more than 200,000 fps results in decreased image quality and abrupt heating of the image sensor and drive circuit board, faster speeds cannot be achieved merely by increasing the drive frequency. We therefore had to improve the image sensor wiring layout and the driving method to develop a new 2M-fps, 300k-pixel ultrahigh-speed single-chip color camera for broadcasting purposes.

Integrated sensor with frame memory and programmable resolution for light adaptive imaging

NASA Technical Reports Server (NTRS)

Zhou, Zhimin (Inventor); Fossum, Eric R. (Inventor); Pain, Bedabrata (Inventor)

2004-01-01

An image sensor operable to vary the output spatial resolution according to a received light level while maintaining a desired signal-to-noise ratio. Signals from neighboring pixels in a pixel patch with an adjustable size are added to increase both the image brightness and signal-to-noise ratio. One embodiment comprises a sensor array for receiving input signals, a frame memory array for temporarily storing a full frame, and an array of self-calibration column integrators for uniform column-parallel signal summation. The column integrators are capable of substantially canceling fixed pattern noise.

Column-parallel correlated multiple sampling circuits for CMOS image sensors and their noise reduction effects.

PubMed

Suh, Sungho; Itoh, Shinya; Aoyama, Satoshi; Kawahito, Shoji

2010-01-01

For low-noise complementary metal-oxide-semiconductor (CMOS) image sensors, the reduction of pixel source follower noises is becoming very important. Column-parallel high-gain readout circuits are useful for low-noise CMOS image sensors. This paper presents column-parallel high-gain signal readout circuits, correlated multiple sampling (CMS) circuits and their noise reduction effects. In the CMS, the gain of the noise cancelling is controlled by the number of samplings. It has a similar effect to that of an amplified CDS for the thermal noise but is a little more effective for 1/f and RTS noises. Two types of the CMS with simple integration and folding integration are proposed. In the folding integration, the output signal swing is suppressed by a negative feedback using a comparator and one-bit D-to-A converter. The CMS circuit using the folding integration technique allows to realize a very low-noise level while maintaining a wide dynamic range. The noise reduction effects of their circuits have been investigated with a noise analysis and an implementation of a 1Mpixel pinned photodiode CMOS image sensor. Using 16 samplings, dynamic range of 59.4 dB and noise level of 1.9 e(-) for the simple integration CMS and 75 dB and 2.2 e(-) for the folding integration CMS, respectively, are obtained.

ELECTROSTRICTION VALVE

DOEpatents

Kippenhan, D.O.

1962-09-25

An accurately controlled, pulse gas valve is designed capable of delivering output pulses which vary in length from one-tenth millisecond to one second or more, repeated at intervals of a few milliseconds or- more. The pulsed gas valve comprises a column formed of barium titanate discs mounted in stacked relation and electrically connected in parallel, with means for applying voltage across the discs to cause them to expand and effect a mechanical elongation axially of the column. The column is mounted within an enclosure having an inlet port and an outlet port with an internal seat in communication with the outlet port, such that a plug secured to the end of the column will engage the seat of the outlet port to close the outlet port in response to the application of voltage is regulated by a conventional electronic timing circuit connected to the column. (AEC)

Improved Underwater Excitation-Emission Matrix Fluorometer

NASA Technical Reports Server (NTRS)

Moore, Casey; daCunha, John; Rhoades, Bruce; Twardowski, Michael

2007-01-01

A compact, high-resolution, two-dimensional excitation-emission matrix fluorometer (EEMF) has been designed and built specifically for use in identifying and measuring the concentrations of organic compounds, including polluting hydrocarbons, in natural underwater settings. Heretofore, most EEMFs have been designed and built for installation in laboratories, where they are used to analyze the contents of samples collected in the field and brought to the laboratories. Because the present EEMF can be operated in the field, it is better suited to measurement of spatially and temporally varying concentrations of substances of interest. In excitation-emission matrix (EEM) fluorometry, fluorescence is excited by irradiating a sample at one or more wavelengths, and the fluorescent emission from the sample is measured at multiple wavelengths. When excitation is provided at only one wavelength, the technique is termed one-dimensional (1D) EEM fluorometry because the resulting matrix of fluorescence emission data (the EEM) contains only one row or column. When excitation is provided at multiple wavelengths, the technique is termed two-dimensional (2D) EEM fluorometry because the resulting EEM contains multiple rows and columns. EEM fluorometry - especially the 2D variety - is well established as a means of simultaneously detecting numerous dissolved and particulate compounds in water. Each compound or pool of compounds has a unique spectral fluorescence signature, and each EEM is rich in information content, in that it can contain multiple fluorescence signatures. By use of deconvolution and/or other mixture-analyses techniques, it is often possible to isolate the spectral signature of compounds of interest, even when their fluorescence spectra overlap. What distinguishes the present 2D EEMF over prior laboratory-type 2D EEMFs are several improvements in packaging (including a sealed housing) and other aspects of design that render it suitable for use in natural underwater settings. In addition, the design of the present 2D EEMF incorporates improvements over the one prior commercial underwater 2D EEMF, developed in 1994 by the same company that developed the present one. Notable advanced features of the present EEMF include the following: 1) High sensitivity and spectral resolution are achieved by use of an off-the-shelf grating spectrometer equipped with a sensor in the form of a commercial astronomical- grade 256 532-pixel charge-coupled-device (CCD) array. 2) All of the power supply, timing, control, and readout circuits for the illumination source and the CCD, ancillary environmental monitoring sensors, and circuitry for controlling a shutter or filter motor are custom-designed and mounted compactly on three circuit boards below a fourth circuit board that holds the CCD (see figure). 3) The compactness of the grating spectrometer, CCD, and circuit assembly makes it possible to fit the entire instrument into a compact package that is intended to be maneuverable underwater by one person. 4) In mass production, the cost of the complete instrument would be relatively low - estimated at approximately $30,000 at 2005 prices.

Diffraction mode terahertz tomography

DOEpatents

Ferguson, Bradley; Wang, Shaohong; Zhang, Xi-Cheng

2006-10-31

A method of obtaining a series of images of a three-dimensional object. The method includes the steps of transmitting pulsed terahertz (THz) radiation through the entire object from a plurality of angles, optically detecting changes in the transmitted THz radiation using pulsed laser radiation, and constructing a plurality of imaged slices of the three-dimensional object using the detected changes in the transmitted THz radiation. The THz radiation is transmitted through the object as a two-dimensional array of parallel rays. The optical detection is an array of detectors such as a CCD sensor.

Establishing column batch repeatability according to Quality by Design (QbD) principles using modeling software.

PubMed

Rácz, Norbert; Kormány, Róbert; Fekete, Jenő; Molnár, Imre

2015-04-10

Column technology needs further improvement even today. To get information of batch-to-batch repeatability, intelligent modeling software was applied. Twelve columns from the same production process, but from different batches were compared in this work. In this paper, the retention parameters of these columns with real life sample solutes were studied. The following parameters were selected for measurements: gradient time, temperature and pH. Based on calculated results, batch-to-batch repeatability of BEH columns was evaluated. Two parallel measurements on two columns from the same batch were performed to obtain information about the quality of packing. Calculating the average of individual working points at the highest critical resolution (R(s,crit)) it was found that the robustness, calculated with a newly released robustness module, had a success rate >98% among the predicted 3(6) = 729 experiments for all 12 columns. With the help of retention modeling all substances could be separated independently from the batch and/or packing, using the same conditions, having high robustness of the experiments. Copyright © 2015 Elsevier B.V. All rights reserved.

Characterization of multiport solid state imagers at megahertz data rates

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yates, G.J.; Pena, C.R.; Turko, B.T.

1994-08-01

Test results obtained from two recently developed multiport Charge-Coupled Devices (CCDs) operated at pixel rates in the 10-to-100 MHz range will be presented . The CCDs were evaluated in Los Alamos National Laboratory`s High Speed Solid State Imager Test Station (HSTS) which features PC-based programmable clock waveform generation (Tektronix DAS 9200) and synchronously clocked Digital Sampling Oscilloscopes (DSOs) (LeCroy 9424/9314 series) for CCD pixel data acquisition, analysis and storage. The HSTS also provided special designed optical pinhole array test patterns in the 5-to-50 micron diameter range for use with Xenon Strobe and pulsed laser light sources to simultaneously provide multiplemore » single-pixel illumination patterns to study CCD point-spread-function (PSF) and pixel smear characteristics. The two CCDs tested, EEV model CCD-13 and EG&G Reticon model HSO512J, are both 512 {times} 512 pixel arrays with eight (8) and sixteen (16) video output ports respectively. Both devices are generically Frame Transfer CCDs (FT CCDs) designed for parallel bi-directional vertical readout to augment their multiport design for increased pixel rates over common single port serial readout architecture. Although both CCDs were tested similarly, differences in their designs precluded normalization or any direct comparisons of test results. Rate dependent parameters investigated include S/N, PSF, and MTF. The performance observed for the two imagers at various pixel rates from selected typical output ports is discussed.« less

Photovoltaic cell array

NASA Technical Reports Server (NTRS)

Eliason, J. T. (Inventor)

1976-01-01

A photovoltaic cell array consisting of parallel columns of silicon filaments is described. Each fiber is doped to produce an inner region of one polarity type and an outer region of an opposite polarity type to thereby form a continuous radial semi conductor junction. Spaced rows of electrical contacts alternately connect to the inner and outer regions to provide a plurality of electrical outputs which may be combined in parallel or in series.

Using Perturbed QR Factorizations To Solve Linear Least-Squares Problems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Avron, Haim; Ng, Esmond G.; Toledo, Sivan

2008-03-21

We propose and analyze a new tool to help solve sparse linear least-squares problems min{sub x} {parallel}Ax-b{parallel}{sub 2}. Our method is based on a sparse QR factorization of a low-rank perturbation {cflx A} of A. More precisely, we show that the R factor of {cflx A} is an effective preconditioner for the least-squares problem min{sub x} {parallel}Ax-b{parallel}{sub 2}, when solved using LSQR. We propose applications for the new technique. When A is rank deficient we can add rows to ensure that the preconditioner is well-conditioned without column pivoting. When A is sparse except for a few dense rows we canmore » drop these dense rows from A to obtain {cflx A}. Another application is solving an updated or downdated problem. If R is a good preconditioner for the original problem A, it is a good preconditioner for the updated/downdated problem {cflx A}. We can also solve what-if scenarios, where we want to find the solution if a column of the original matrix is changed/removed. We present a spectral theory that analyzes the generalized spectrum of the pencil (A*A,R*R) and analyze the applications.« less

A parallel-vector algorithm for rapid structural analysis on high-performance computers

NASA Technical Reports Server (NTRS)

Storaasli, Olaf O.; Nguyen, Duc T.; Agarwal, Tarun K.

1990-01-01

A fast, accurate Choleski method for the solution of symmetric systems of linear equations is presented. This direct method is based on a variable-band storage scheme and takes advantage of column heights to reduce the number of operations in the Choleski factorization. The method employs parallel computation in the outermost DO-loop and vector computation via the 'loop unrolling' technique in the innermost DO-loop. The method avoids computations with zeros outside the column heights, and as an option, zeros inside the band. The close relationship between Choleski and Gauss elimination methods is examined. The minor changes required to convert the Choleski code to a Gauss code to solve non-positive-definite symmetric systems of equations are identified. The results for two large-scale structural analyses performed on supercomputers, demonstrate the accuracy and speed of the method.

A parallel-vector algorithm for rapid structural analysis on high-performance computers

NASA Technical Reports Server (NTRS)

Storaasli, Olaf O.; Nguyen, Duc T.; Agarwal, Tarun K.

1990-01-01

A fast, accurate Choleski method for the solution of symmetric systems of linear equations is presented. This direct method is based on a variable-band storage scheme and takes advantage of column heights to reduce the number of operations in the Choleski factorization. The method employs parallel computation in the outermost DO-loop and vector computation via the loop unrolling technique in the innermost DO-loop. The method avoids computations with zeros outside the column heights, and as an option, zeros inside the band. The close relationship between Choleski and Gauss elimination methods is examined. The minor changes required to convert the Choleski code to a Gauss code to solve non-positive-definite symmetric systems of equations are identified. The results for two large scale structural analyses performed on supercomputers, demonstrate the accuracy and speed of the method.

Design and performance of 4 x 5120-element visible and 2 x 2560-element shortwave infrared multispectral focal planes

NASA Astrophysics Data System (ADS)

Tower, J. R.; Cope, A. D.; Pellon, L. E.; McCarthy, B. M.; Strong, R. T.

1986-06-01

Two solid-state sensors for use in remote sensing instruments operating in the pushbroom mode are examined. The design and characteristics of the visible/near-infrared (VIS/NIR) device and the short-wavelength infrared (SWIR) device are described. The VIS/NIR is a CCD imager with four parallel sensor lines, each 1024 pixel long; the chip design and filter system of the VIS/NIR are studied. The performance of the VIS/NIR sensor with mask and its system performance are measured. The SWIR is a dual-band line imager consisting of palladium silicide Schottky-barrier detectors coupled to CCD multiplexers; the performance of the device is analyzed. The substrate materials and layout designs used to assemble the 4 x 5120-element VIS/NIR array and the 2 x 2560-element SWIR array are discussed, and the planarity of the butted arrays are verified using a profilometer. The optical and electrical characteristics, and the placement and butting accuracy of the arrays are evaluated. It is noted that the arrays met or exceed their expected performance.

ART-XC: A Medium-energy X-ray Telescope System for the Spectrum-R-Gamma Mission

NASA Technical Reports Server (NTRS)

Arefiev, V.; Pavlinsky, M.; Lapshov, I.; Thachenko, A.; Sazonov, S.; Revnivtsev, M.; Semena, N.; Buntov,M.; Vikhlinin, A.; Gubarev, M.;

Fast Confocal Raman Imaging Using a 2-D Multifocal Array for Parallel Hyperspectral Detection.

PubMed

Kong, Lingbo; Navas-Moreno, Maria; Chan, James W

2016-01-19

We present the development of a novel confocal hyperspectral Raman microscope capable of imaging at speeds up to 100 times faster than conventional point-scan Raman microscopy under high noise conditions. The microscope utilizes scanning galvomirrors to generate a two-dimensional (2-D) multifocal array at the sample plane, generating Raman signals simultaneously at each focus of the array pattern. The signals are combined into a single beam and delivered through a confocal pinhole before being focused through the slit of a spectrometer. To separate the signals from each row of the array, a synchronized scan mirror placed in front of the spectrometer slit positions the Raman signals onto different pixel rows of the detector. We devised an approach to deconvolve the superimposed signals and retrieve the individual spectra at each focal position within a given row. The galvomirrors were programmed to scan different focal arrays following Hadamard encoding patterns. A key feature of the Hadamard detection is the reconstruction of individual spectra with improved signal-to-noise ratio. Using polystyrene beads as test samples, we demonstrated not only that our system images faster than a conventional point-scan method but that it is especially advantageous under noisy conditions, such as when the CCD detector operates at fast read-out rates and high temperatures. This is the first demonstration of multifocal confocal Raman imaging in which parallel spectral detection is implemented along both axes of the CCD detector chip. We envision this novel 2-D multifocal spectral detection technique can be used to develop faster imaging spontaneous Raman microscopes with lower cost detectors.

Seasonal and Interannual Variabilities in Tropical Tropospheric Ozone

NASA Technical Reports Server (NTRS)

Ziemke, J. R.; Chandra, S.

1999-01-01

This paper presents a detailed characterization of seasonal and interannual variability in tropical tropospheric column ozone (TCO). TCO time series are derived from 20 years (1979-1998) of total ozone mapping spectrometer (TOMS) data using the convective cloud differential (CCD) method. Our study identifies three regions in the tropics with distinctly different zonal characteristics related to seasonal and interannual variability. These three regions are the eastern Pacific, Atlantic, and western Pacific. Results show that in both the eastern and western Pacific seasonal-cycle variability of northern hemisphere (NH) TCO exhibits maximum amount during NH spring whereas largest amount in southern hemisphere (SH) TCO occurs during SH spring. In the Atlantic, maximum TCO in both hemispheres occurs in SH spring. These seasonal cycles are shown to be comparable to seasonal cycles present in ground-based ozonesonde measurements. Interannual variability in the Atlantic region indicates a quasi-biennial oscillation (QBO) signal that is out of phase with the QBO present in stratospheric column ozone (SCO). This is consistent with high pollution and high concentrations of mid-to-upper tropospheric O3-producing precursors in this region. The out of phase relation suggests a UV modulation of tropospheric photochemistry caused by the QBO in stratospheric O3. During El Nino events there is anomalously low TCO in the eastern Pacific and high values in the western Pacific, indicating the effects of convectively-driven transport of low-value boundary layer O3 (reducing TCO) and O3 precursors including H2O and OH. A simplified technique is proposed to derive high-resolution maps of TCO in the tropics even in the absence of tropopause-level clouds. This promising approach requires only total ozone gridded measurements and utilizes the small variability observed in TCO near the dateline. This technique has an advantage compared to the CCD method because the latter requires high-resolution footprint measurements of both reflectivity and total ozone in the presence of tropopause-level cloud tops.

Recombinant Human Acidic Fibroblast Growth Factor (aFGF) Expressed in Nicotiana benthamiana Potentially Inhibits Skin Photoaging.

PubMed

Ha, Jang-Ho; Kim, Ha-Neul; Moon, Ki-Beom; Jeon, Jae-Heung; Jung, Dai-Hyun; Kim, Su-Jung; Mason, Hugh S; Shin, Seo-Yeon; Kim, Hyun-Soon; Park, Kyung-Mok

2017-07-01

Responding to the need for recombinant acidic fibroblast growth factor in the pharmaceutical and cosmetic industries, we established a scalable expression system for recombinant human aFGF using transient and a DNA replicon vector expression in Nicotiana benthamiana . Recombinant human-acidic fibroblast growth factor was recovered following Agrobacterium infiltration of N. benthamiana . The optimal time point at which to harvest recombinant human acidic fibroblast growth factor expressing leaves was found to be 4 days post-infiltration, before necrosis was evident. Commassie-stained SDS-PAGE gels of His-tag column eluates, concentrated using a 10 000 molecular weight cut-off column, showed an intense band at the expected molecular weight for recombinant human acidic fibroblast growth factor. An immunoblot confirmed that this band was recombinant human acidic fibroblast growth factor. Up to 10 µg recombinant human-acidic fibroblast growth factor/g of fresh leaves were achieved by a simple affinity purification protocol using protein extract from the leaves of agroinfiltrated N. benthamiana . The purified recombinant human acidic fibroblast growth factor improved the survival rate of UVB-irradiated HaCaT and CCD-986sk cells approximately 89 and 81 %, respectively. N. benthamiana -derived recombinant human acidic fibroblast growth factor showed similar effects on skin cell proliferation and UVB protection compared to those of Escherichia coli -derived recombinant human acidic fibroblast growth factor. Additionally, N. benthamiana- derived recombinant human acidic fibroblast growth factor increased type 1 procollagen synthesis up to 30 % as well as reduced UVB-induced intracellular reactive oxygen species generation in fibroblast (CCD-986sk) cells.UVB is a well-known factor that causes various types of skin damage and premature aging. Therefore, the present study demonstrated that N. benthamiana -derived recombinant human acidic fibroblast growth factor effectively protects skin cell from UVB, suggesting its potential use as a cosmetic or therapeutic agent against skin photoaging. Georg Thieme Verlag KG Stuttgart · New York.

Online measurement for geometrical parameters of wheel set based on structure light and CUDA parallel processing

NASA Astrophysics Data System (ADS)

Wu, Kaihua; Shao, Zhencheng; Chen, Nian; Wang, Wenjie

2018-01-01

The wearing degree of the wheel set tread is one of the main factors that influence the safety and stability of running train. Geometrical parameters mainly include flange thickness and flange height. Line structure laser light was projected on the wheel tread surface. The geometrical parameters can be deduced from the profile image. An online image acquisition system was designed based on asynchronous reset of CCD and CUDA parallel processing unit. The image acquisition was fulfilled by hardware interrupt mode. A high efficiency parallel segmentation algorithm based on CUDA was proposed. The algorithm firstly divides the image into smaller squares, and extracts the squares of the target by fusion of k_means and STING clustering image segmentation algorithm. Segmentation time is less than 0.97ms. A considerable acceleration ratio compared with the CPU serial calculation was obtained, which greatly improved the real-time image processing capacity. When wheel set was running in a limited speed, the system placed alone railway line can measure the geometrical parameters automatically. The maximum measuring speed is 120km/h.

Site 766: Sedimentology

USGS Publications Warehouse

,

1990-01-01

Site 766 is located at the base of the steep western margin of the Exmouth Plateau. The oldest sediment penetrated at Site 766, in Section 123-766A-49R-4 at 66 cm (466.7 mbsf), is uppermost Valanginian sandstone and siltstone, alternating with inclined basaltic intrusions (see "Igneous Rock Lithostratigraphy" section, this chapter). The uppermost sediment/basalt interface occurs in Section 123-766A-48R-6 at 129 cm (460.6 mbsf) At least 300 m (approximately 65%) of the sediments penetrated accumulated during the Lower Cretaceous, compared with less than 150 m thereafter. At Site 765, on the Argo Abyssal Plain, the Lower Cretaceous also is slightly more than 300 m thick. However, approximately 65% of the total sediment column at this site accumulated after the Lower Cretaceous, primarily during the Neogene. The sedimentation history, based on the age and present depth of basement(?) and time-depth relationship for oceanic crust, suggests that Site 766 began at a depth of about 800 m. However, the presence of shallow marine components in the oldest lithologic unit, if not redeposited, suggests that initial depths were shallower. Site 766 appears to have remained above or near the carbonate compensation depth (CCD) throughout its history, whereas Site 765 may have started near the CCD, but remained below it throughout most of its history.

Development of an optical inspection platform for surface defect detection in touch panel glass

NASA Astrophysics Data System (ADS)

Chang, Ming; Chen, Bo-Cheng; Gabayno, Jacque Lynn; Chen, Ming-Fu

2016-04-01

An optical inspection platform combining parallel image processing with high resolution opto-mechanical module was developed for defect inspection of touch panel glass. Dark field images were acquired using a 12288-pixel line CCD camera with 3.5 µm per pixel resolution and 12 kHz line rate. Key features of the glass surface were analyzed by parallel image processing on combined CPU and GPU platforms. Defect inspection of touch panel glass, which provided 386 megapixel image data per sample, was completed in roughly 5 seconds. High detection rate of surface scratches on the touch panel glass was realized with minimum defects size of about 10 µm after inspection. The implementation of a custom illumination source significantly improved the scattering efficiency on the surface, therefore enhancing the contrast in the acquired images and overall performance of the inspection system.

Follow-up study of children with cerebral coordination disturbance (CCD, Vojta).

PubMed

Imamura, S; Sakuma, K; Takahashi, T

1983-01-01

713 children (from newborn to 12-month-old) with delayed motor development were carefully examined and classified into normal, very light cerebral coordination disturbance (CCD, Vojta), light CCD, moderate CCD, severe CCD, suspected cerebral palsy (CP) and other diseases at their first visit, and were followed up carefully. Finally, 89.0% of very light CCD, 71.4% of light CCD, 56.0% of moderate CCD and 30.0% of severe CCD developed into normal. 59.5% of moderate CCD and 45.5% of severe CCD among children who were given Vojta's physiotherapy developed into normal. The classification of cases with delayed motor development into very light, light, moderate and severe CCD based on the extent of abnormality in their postural reflexes is useful and well correlated with their prognosis. Treatment by Vojta's method seems to be efficient and helpful for young children with delayed motor development.

Parallel-plate wet denuder coupled ion chromatograph for near-real-time detection of trace acidic gases in clean room air.

PubMed

Takeuchi, Masaki; Tsunoda, Hiromichi; Tanaka, Hideji; Shiramizu, Yoshimi

2011-01-01

This paper describes the performance of our automated acidic (CH(3)COOH, HCOOH, HCl, HNO(2), SO(2), and HNO(3)) gases monitor utilizing a parallel-plate wet denuder (PPWD). The PPWD quantitatively collects gaseous contaminants at a high sample flow rate (∼8 dm(3) min(-1)) compared to the conventional methods used in a clean room. Rapid response to any variability in the sample concentration enables near-real-time monitoring. In the developed monitor, the analyte collected with the PPWD is pumped into one of two preconcentration columns for 15 min, and determined by means of ion chromatography. While one preconcentration column is used for chromatographic separation, the other is used for loading the sample solution. The system allows continuous monitoring of the common acidic gases in an advanced semiconductor manufacturing clean room. 2011 © The Japan Society for Analytical Chemistry

Enhancement of soft X-ray lasing action with thin blade radiators

DOEpatents

Suckewer, Szymon; Skinner, Charles H.; Voorhees, David R.

1988-01-01

An enhancement of approximately 100 of stimulated emission over spontaneous emission of the CVI 182 Angstrom line was obtained in a recombining magnetically confined plasma column. The plasma was formed by focusing a CO.sub.2 laser beam on a carbon disc. A magnetic solenoid produced a strong magnetic field which confined the plasma to the shape of a column. A single thin carbon blade extended parallel to the plasma column and served to make the column axially more uniform and also acted as a heat sink. Axial and transverse measurements of the soft X-ray lasing action were made from locations off-set from the central axis of the plasma column. Multiple carbon blades located at equal intervals around the plasma column were also found to produce acceptable results. According to another embodiment 10 a thin coating of aluminum or magnesium was placed on the carbon disc and blade. The Z of the coating should preferably be at least 5 greater than the Z of the target. Measurements of the soft X-rays generated at 182 Angstroms showed a significant increase in intensity enhancement.

High-throughput NGL electron-beam direct-write lithography system

NASA Astrophysics Data System (ADS)

Parker, N. William; Brodie, Alan D.; McCoy, John H.

2000-07-01

Electron beam lithography systems have historically had low throughput. The only practical solution to this limitation is an approach using many beams writing simultaneously. For single-column multi-beam systems, including projection optics (SCALPELR and PREVAIL) and blanked aperture arrays, throughput and resolution are limited by space-charge effects. Multibeam micro-column (one beam per column) systems are limited by the need for low voltage operation, electrical connection density and fabrication complexities. In this paper, we discuss a new multi-beam concept employing multiple columns each with multiple beams to generate a very large total number of parallel writing beams. This overcomes the limitations of space-charge interactions and low voltage operation. We also discuss a rationale leading to the optimum number of columns and beams per column. Using this approach we show how production throughputs >= 60 wafers per hour can be achieved at CDs

Measurements of stress evolution during thin film deposition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chason, E.; Floro, J.A.

1996-05-01

We have developed a technique for measuring thin film stress during growth by monitoring the wafer curvature. By measuring the deflection of multiple parallel laser beams with a CCD detector, the sensivity to vibration is reduced and a radius of curvature limit of 4 km has been obtained in situ. This technique also enables us to obtain a 2-dimensional profile of the surface curvature from the simultaneous reflection of a rectangular array of beams. Results from the growth of SiG alloy films are presented to demonstrate the unique information that can be obtained during growth.

Computing row and column counts for sparse QR and LU factorization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gilbert, John R.; Li, Xiaoye S.; Ng, Esmond G.

2001-01-01

We present algorithms to determine the number of nonzeros in each row and column of the factors of a sparse matrix, for both the QR factorization and the LU factorization with partial pivoting. The algorithms use only the nonzero structure of the input matrix, and run in time nearly linear in the number of nonzeros in that matrix. They may be used to set up data structures or schedule parallel operations in advance of the numerical factorization. The row and column counts we compute are upper bounds on the actual counts. If the input matrix is strong Hall and theremore » is no coincidental numerical cancellation, the counts are exact for QR factorization and are the tightest bounds possible for LU factorization. These algorithms are based on our earlier work on computing row and column counts for sparse Cholesky factorization, plus an efficient method to compute the column elimination tree of a sparse matrix without explicitly forming the product of the matrix and its transpose.« less

Science from Kepler Collateral Data: 50 Kilosecond per Year from 13 Million Stars

NASA Technical Reports Server (NTRS)

Kolodziejczak, J. J.; Caldwell, D. A.

2012-01-01

As each Kepler frame is read out, light from each star in a CCD column accumulates in successive pixels as they wait for the next row to be read out. This accumulation is the same in the masked rows at the start of the readout and virtual rows at the end of the readout as it is in the science data. A range of these "smear" rows are added together for each long cadence and sent to the ground for calibration purposes. We will introduce and describe this smear collateral data, discuss and demonstrate its potential use for scientific studies exclusive of Kepler calibration,[1,2] including global characteristics of stellar variability, which are influenced by parameters of galactic evolution.

Mapper: high throughput maskless lithography

NASA Astrophysics Data System (ADS)

Kuiper, V.; Kampherbeek, B. J.; Wieland, M. J.; de Boer, G.; ten Berge, G. F.; Boers, J.; Jager, R.; van de Peut, T.; Peijster, J. J. M.; Slot, E.; Steenbrink, S. W. H. K.; Teepen, T. F.; van Veen, A. H. V.

2009-01-01

Maskless electron beam lithography, or electron beam direct write, has been around for a long time in the semiconductor industry and was pioneered from the mid-1960s onwards. This technique has been used for mask writing applications as well as device engineering and in some cases chip manufacturing. However because of its relatively low throughput compared to optical lithography, electron beam lithography has never been the mainstream lithography technology. To extend optical lithography double patterning, as a bridging technology, and EUV lithography are currently explored. Irrespective of the technical viability of both approaches, one thing seems clear. They will be expensive [1]. MAPPER Lithography is developing a maskless lithography technology based on massively-parallel electron-beam writing with high speed optical data transport for switching the electron beams. In this way optical columns can be made with a throughput of 10-20 wafers per hour. By clustering several of these columns together high throughputs can be realized in a small footprint. This enables a highly cost-competitive alternative to double patterning and EUV alternatives. In 2007 MAPPER obtained its Proof of Lithography milestone by exposing in its Demonstrator 45 nm half pitch structures with 110 electron beams in parallel, where all the beams where individually switched on and off [2]. In 2008 MAPPER has taken a next step in its development by building several tools. A new platform has been designed and built which contains a 300 mm wafer stage, a wafer handler and an electron beam column with 110 parallel electron beams. This manuscript describes the first patterning results with this 300 mm platform.

Implementing parallel spreadsheet models for health policy decisions: The impact of unintentional errors on model projections

PubMed Central

Bailey, Stephanie L.; Bono, Rose S.; Nash, Denis; Kimmel, April D.

2018-01-01

Background Spreadsheet software is increasingly used to implement systems science models informing health policy decisions, both in academia and in practice where technical capacity may be limited. However, spreadsheet models are prone to unintentional errors that may not always be identified using standard error-checking techniques. Our objective was to illustrate, through a methodologic case study analysis, the impact of unintentional errors on model projections by implementing parallel model versions. Methods We leveraged a real-world need to revise an existing spreadsheet model designed to inform HIV policy. We developed three parallel versions of a previously validated spreadsheet-based model; versions differed by the spreadsheet cell-referencing approach (named single cells; column/row references; named matrices). For each version, we implemented three model revisions (re-entry into care; guideline-concordant treatment initiation; immediate treatment initiation). After standard error-checking, we identified unintentional errors by comparing model output across the three versions. Concordant model output across all versions was considered error-free. We calculated the impact of unintentional errors as the percentage difference in model projections between model versions with and without unintentional errors, using +/-5% difference to define a material error. Results We identified 58 original and 4,331 propagated unintentional errors across all model versions and revisions. Over 40% (24/58) of original unintentional errors occurred in the column/row reference model version; most (23/24) were due to incorrect cell references. Overall, >20% of model spreadsheet cells had material unintentional errors. When examining error impact along the HIV care continuum, the percentage difference between versions with and without unintentional errors ranged from +3% to +16% (named single cells), +26% to +76% (column/row reference), and 0% (named matrices). Conclusions Standard error-checking techniques may not identify all errors in spreadsheet-based models. Comparing parallel model versions can aid in identifying unintentional errors and promoting reliable model projections, particularly when resources are limited. PMID:29570737

Implementing parallel spreadsheet models for health policy decisions: The impact of unintentional errors on model projections.

PubMed

Bailey, Stephanie L; Bono, Rose S; Nash, Denis; Kimmel, April D

2018-01-01

Spreadsheet software is increasingly used to implement systems science models informing health policy decisions, both in academia and in practice where technical capacity may be limited. However, spreadsheet models are prone to unintentional errors that may not always be identified using standard error-checking techniques. Our objective was to illustrate, through a methodologic case study analysis, the impact of unintentional errors on model projections by implementing parallel model versions. We leveraged a real-world need to revise an existing spreadsheet model designed to inform HIV policy. We developed three parallel versions of a previously validated spreadsheet-based model; versions differed by the spreadsheet cell-referencing approach (named single cells; column/row references; named matrices). For each version, we implemented three model revisions (re-entry into care; guideline-concordant treatment initiation; immediate treatment initiation). After standard error-checking, we identified unintentional errors by comparing model output across the three versions. Concordant model output across all versions was considered error-free. We calculated the impact of unintentional errors as the percentage difference in model projections between model versions with and without unintentional errors, using +/-5% difference to define a material error. We identified 58 original and 4,331 propagated unintentional errors across all model versions and revisions. Over 40% (24/58) of original unintentional errors occurred in the column/row reference model version; most (23/24) were due to incorrect cell references. Overall, >20% of model spreadsheet cells had material unintentional errors. When examining error impact along the HIV care continuum, the percentage difference between versions with and without unintentional errors ranged from +3% to +16% (named single cells), +26% to +76% (column/row reference), and 0% (named matrices). Standard error-checking techniques may not identify all errors in spreadsheet-based models. Comparing parallel model versions can aid in identifying unintentional errors and promoting reliable model projections, particularly when resources are limited.

Relaxation of flux ropes and magnetic reconnection in the Reconnection Scaling Experiment at LANL

NASA Astrophysics Data System (ADS)

Furno, I.; Intrator, T.; Hemsing, E.; Hsu, S.; Lapenta, G.; Abbate, S.

2004-12-01

Magnetic reconnection and plasma relaxation are studied in the Reconnection Scaling Experiment (RSX) with current carrying plasma columns (magnetic flux ropes). Using plasma guns, multiple flux ropes (Bθ ≤ 100 Gauss, L=90 cm, r≤3 cm) are generated in a three-dimensional (3D) cylindrical geometry and are observed to evolve dynamically during the injection of magnetic helicity. Detailed evolution of electron density, temperature, plasma potential and magnetic field structures is reconstructed experimentally and visible light emission is captured with a fast-gated, intensified CCD camera to provide insight into the global flux rope dynamics. Experiments with two flux ropes in collisional plasmas and in a strong axial guide field (Bz / Bθ > 10) suggest that magnetic reconnection plays an important role in the initial stages of flux rope evolution. During the early stages of the applied current drive (t≤ 20 τ Alfv´ {e}n), the flux ropes are observed to twist, partially coalesce and form a thin current sheet with a scale size comparable to that of the ion sound gyro-radius. Here, non-ideal terms in a generalized Ohm's Law appear to play a significant role in the 3D reconnection process as shown by the presence of a strong axial pressure gradient in the current sheet. In addition, a density perturbation with a structure characteristic of a kinetic Alfvén wave is observed to propagate axially in the current layer, anti-parallel to the induced sheet current. Later in the evolution, when a sufficient amount of helicity is injected into the system, a critical threshold for the kink instability is exceeded and the helical twisting of each individual flux rope can dominate the dynamics of the system. This may prevent the complete coalescence of the flux ropes.

Relaxation of flux ropes and magnetic reconnection in the Reconnection Scaling Experiment at LANL

NASA Astrophysics Data System (ADS)

Furno, Ivo

2004-11-01

Magnetic reconnection and plasma relaxation are studied in the Reconnection Scaling Experiment (RSX) with current carrying plasma columns (magnetic flux ropes). Using plasma guns, multiple flux ropes (B_pol < 100 Gauss, L=90 cm, r < 3 cm) are generated in a three-dimensional (3D) cylindrical geometry and are observed to evolve dynamically during the injection of magnetic helicity. Detailed evolution of electron density, temperature, plasma potential and magnetic field structures is reconstructed experimentally and visible light emission is captured with a fast-gated, intensified CCD camera to provide insight into the global flux rope dynamics. Experiments with two flux ropes in collisional plasmas and in a strong axial guide field (Bz / B_pol > 10) suggest that magnetic reconnection plays an important role in the initial stages of flux rope evolution. During the early stages of the applied current drive (t < 20τ_Alfven), the flux ropes are observed to twist, partially coalesce and form a thin current sheet with a scale size comparable to that of the ion sound gyro-radius. Here, non-ideal terms in a generalized Ohm's Law appear to play a significant role in the 3D reconnection process as shown by the presence of a strong axial pressure gradient in the current sheet. In addition, a density perturbation with a structure characteristic of a kinetic Alfvén wave is observed to propagate axially in the current layer, anti-parallel to the induced sheet current. Later in the evolution, when a sufficient amount of helicity is injected into the system, a critical threshold for the kink instability is exceeded and the helical twisting of each individual flux rope can dominate the dynamics of the system. This may prevent the complete coalescence of the flux ropes.

A CMOS high speed imaging system design based on FPGA

NASA Astrophysics Data System (ADS)

Tang, Hong; Wang, Huawei; Cao, Jianzhong; Qiao, Mingrui

2015-10-01

CMOS sensors have more advantages than traditional CCD sensors. The imaging system based on CMOS has become a hot spot in research and development. In order to achieve the real-time data acquisition and high-speed transmission, we design a high-speed CMOS imaging system on account of FPGA. The core control chip of this system is XC6SL75T and we take advantages of CameraLink interface and AM41V4 CMOS image sensors to transmit and acquire image data. AM41V4 is a 4 Megapixel High speed 500 frames per second CMOS image sensor with global shutter and 4/3" optical format. The sensor uses column parallel A/D converters to digitize the images. The CameraLink interface adopts DS90CR287 and it can convert 28 bits of LVCMOS/LVTTL data into four LVDS data stream. The reflected light of objects is photographed by the CMOS detectors. CMOS sensors convert the light to electronic signals and then send them to FPGA. FPGA processes data it received and transmits them to upper computer which has acquisition cards through CameraLink interface configured as full models. Then PC will store, visualize and process images later. The structure and principle of the system are both explained in this paper and this paper introduces the hardware and software design of the system. FPGA introduces the driven clock of CMOS. The data in CMOS is converted to LVDS signals and then transmitted to the data acquisition cards. After simulation, the paper presents a row transfer timing sequence of CMOS. The system realized real-time image acquisition and external controls.

Application of response surface methodology (RSM) to the optimization of a post-column luminol chemiluminescence analysis of silyl peroxides.

PubMed

Baj, Stefan; Słupska, Roksana; Krawczyk, Tomasz

2013-01-15

The possibility of the utilization of chemiluminescence post-column luminol oxidation (CL) in a HPLC system for silyl peroxides analysis has been investigated. The conditions of HPLC separation for 12 silyl peroxides, representing bissilyl and alkyl-silyl peroxides, as well as their potential impurities, were established. Optimal chemiluminescent post-column reaction conditions were found using central composite design (CCD) and response surface methodology (RSM). The interaction effects of four of the most important operating variables - the concentrations of luminol, hemin, sodium hydroxide and the post-column solution flow rate - on the light intensity were evaluated. The optimized conditions for analysis were the same for bissilyl and alkyl-silyl peroxides for the base concentration (0.03 M), the luminol concentration (0.4 g L(-1)) and the hemin concentration (0.3 g L(-1)). The only differences occurred in a reagent flow rate (for bissilyl peroxide -0.3 mL min(-1) and for alkyl-silyl peroxides -0.9 mL min(-1)). Under optimal conditions, the detection limits were in the 0.07-0.16 nM range for bissilyl, and 0.53-1.01 for alkyl-silyl peroxides. The calibration curves were linear in the 0.25-3 nM range for bissilyl and the 2.5-25 range for alkyl-silyl peroxides. Intra-day and inter-day precision was lower than 5.5% for each tested concentration level. A mechanism of luminol oxidation by silyl peroxides involving a hydrolysis step with the formation of hydrogen peroxide or hydroperoxide was proposed. Copyright © 2012 Elsevier B.V. All rights reserved.

Radial dependence of HF wave field strength in the BPD column. [Beam Plasma Discharge

NASA Technical Reports Server (NTRS)

Jost, R. J.; Anderson, H. R.; Bernstein, W.; Kellogg, P. J.

1982-01-01

The results of a recent set of RF frequency measurements of the beam plasma discharge (BPD) performed in order to determine a quantitative value for the field strength in the plasma frequency region of the spectrum are presented. The parallel and perpendicular components of the plasma wave electric fields inside the BPD column have comparable field strengths, on the order of 10 volts/m. The radial dependence of the field strength is very strong, decreasing by as much as 40 dB within one meter from the beam center, with the illumination or discharge column approximately one meter in diameter. The field strength inside the column increases as a function of distance along the beam at least for several meters from the gun aperture. The frequency and amplitude of the plasma wave increases with beam current. A particularly rapid increase in these parameters occurs as the beam current approaches the critical current.

Anaerobic degradation of vinyl chloride in aquifer microcosms.

PubMed

Smits, Theo H M; Assal, Antoine; Hunkeler, Daniel; Holliger, Christof

2011-01-01

The anaerobic degradation potential at a chloroethene-contaminated site was investigated by operating two anoxic column aquifer microcosms enriched in iron(III). One column was fed with vinyl chloride (VC) only (column A) and one with VC and acetate (column B). In column A, after about 600 pore volume exchanges (PVEs), VC started to disappear and reached almost zero VC recovery in the effluent after 1000 PVEs. No formation of ethene was observed. In column B, effluent VC was almost always only a fraction of influent VC. Formation of ethene was observed after 800 PVEs and started to become an important degradation product after 1550 PVEs. However, ethene was never observed in stoichiometric amounts compared with disappeared VC. The average stable isotope enrichment factor for VC disappearance in column A was determined to be -4.3‰. In column B, the isotope enrichment factor shifted from -10.7 to -18.5‰ concurrent with an increase in ethene production. Batch microcosms inoculated with column material showed similar isotope enrichment factors as the column microcosms. These results indicated that two degradation processes occurred, one in column A and two in parallel in column B with increasing importance of reductive dechlorination with time. This study suggests that in addition to reductive dechlorination, other degradation processes such as anaerobic oxidation should be taken into account when evaluating natural attenuation of VC and that isotope analysis can help to differentiate between different pathways of VC removal. American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.

Spatial zonation limits magnesite dissolution in porous media

NASA Astrophysics Data System (ADS)

Li, Li; Salehikhoo, Fatemeh; Brantley, Susan L.; Heidari, Peyman

2014-02-01

We investigate how mineral spatial distribution in porous media affects their dissolution rates. Specifically, we measure the dissolution rate of magnesite interspersed in different patterns in packed columns of quartz sand where the magnesite concentration (v/v) was held constant. The largest difference was observed between a “Mixed column” containing uniformly distributed magnesite and a “One-zone column” containing magnesite packed into one cylindrical center zone aligned parallel to the main flow of acidic inlet fluid (flow-parallel One-zone column). The columns were flushed with acid water at a pH of 4.0 at flow velocities of 3.6 or 0.36 m/d. Breakthrough data show that the rate of magnesite dissolution is 1.6-2 times slower in the One-zone column compared to the Mixed column. This extent of rate limitation is much larger than what was observed in our previous work (14%) for a similar One-zone column where the magnesite was packed in a layer aligned perpendicular to flow (flow-transverse One-zone column). Two-dimensional reactive transport modeling with CrunchFlow revealed that ion activity product (IAP) and local dissolution rates at the grid block scale (0.1 cm) vary by orders of magnitude. Much of the central magnesite zone in the One-zone flow-parallel column is characterized by close or equal to equilibrium conditions with IAP/Keq > 0.1. Two important surface areas are defined to understand the observed rates: the effective surface area (Ae) reflects the magnesite that effectively dissolves under far from equilibrium conditions (IAP/Keq < 0.1), while the interface surface area (AI) reflects the effective magnesite surface that lies along the quartz-magnesite interface. Modeling results reveal that the transverse dispersivity at the interface of the quartz and magnesite zones controls mass transport and therefore the values of Ae and AI. Under the conditions examined in this work, the value of Ae varies from 2% to 67% of the total magnesite BET surface area. Column-scale bulk rates R,B (in units of mol/s) vary linearly with Ae and AI. Using Ae to normalize rates, we calculate a rate constant (10-9.56 mol/m2/s) that is very close to the value of 10-10.0 mol/m2/s under well-mixed conditions at the grid block scale. This implies that the laboratory-field rate discrepancy can potentially be caused by differences in the effective surface area. If we know the effective surface area of dissolution, we will be able to use the rate constant measured in laboratory systems to calculate field rates for some systems. In this work, approximately 60-70% of the Ae is at the magnesite-quartz interface. This implies that in some field systems where the detailed information that we have for our columns is not available, the effective mineral surface area may be approximated by the area of grains residing at the interface of reactive mineral zones. Although it has long been known that spatial heterogeneities play a significant role in determining physical processes such as flow and solute transport, our data are the first that systematically and experimentally quantifies the importance of mineral spatial distribution (chemical heterogeneity) on dissolution.

Color image analysis of contaminants and bacteria transport in porous media

NASA Astrophysics Data System (ADS)

Rashidi, Mehdi; Dehmeshki, Jamshid; Daemi, Mohammad F.; Cole, Larry; Dickenson, Eric

1997-10-01

Transport of contaminants and bacteria in aqueous heterogeneous saturated porous systems have been studied experimentally using a novel fluorescent microscopic imaging technique. The approach involves color visualization and quantification of bacterium and contaminant distributions within a transparent porous column. By introducing stained bacteria and an organic dye as a contaminant into the column and illuminating the porous regions with a planar sheet of laser beam, contaminant and bacterial transport processes through the porous medium can be observed and measured microscopically. A computer controlled color CCD camera is used to record the fluorescent images as a function of time. These images are recorded by a frame accurate high resolution VCR and are then analyzed using a color image analysis code written in our laboratories. The color images are digitized this way and simultaneous concentration and velocity distributions of both contaminant and bacterium are evaluated as a function of time and pore characteristics. The approach provides a unique dynamic probe to observe these transport processes microscopically. These results are extremely valuable in in-situ bioremediation problems since microscopic particle-contaminant- bacterium interactions are the key to understanding and optimization of these processes.

The application of image processing in the measurement for three-light-axis parallelity of laser ranger

NASA Astrophysics Data System (ADS)

Wang, Yang; Wang, Qianqian

2008-12-01

When laser ranger is transported or used in field operations, the transmitting axis, receiving axis and aiming axis may be not parallel. The nonparallelism of the three-light-axis will affect the range-measuring ability or make laser ranger not be operated exactly. So testing and adjusting the three-light-axis parallelity in the production and maintenance of laser ranger is important to ensure using laser ranger reliably. The paper proposes a new measurement method using digital image processing based on the comparison of some common measurement methods for the three-light-axis parallelity. It uses large aperture off-axis paraboloid reflector to get the images of laser spot and white light cross line, and then process the images on LabVIEW platform. The center of white light cross line can be achieved by the matching arithmetic in LABVIEW DLL. And the center of laser spot can be achieved by gradation transformation, binarization and area filter in turn. The software system can set CCD, detect the off-axis paraboloid reflector, measure the parallelity of transmitting axis and aiming axis and control the attenuation device. The hardware system selects SAA7111A, a programmable vedio decoding chip, to perform A/D conversion. FIFO (first-in first-out) is selected as buffer.USB bus is used to transmit data to PC. The three-light-axis parallelity can be achieved according to the position bias between them. The device based on this method has been already used. The application proves this method has high precision, speediness and automatization.

Dedicated hardware processor and corresponding system-on-chip design for real-time laser speckle imaging.

PubMed

Jiang, Chao; Zhang, Hongyan; Wang, Jia; Wang, Yaru; He, Heng; Liu, Rui; Zhou, Fangyuan; Deng, Jialiang; Li, Pengcheng; Luo, Qingming

2011-11-01

Laser speckle imaging (LSI) is a noninvasive and full-field optical imaging technique which produces two-dimensional blood flow maps of tissues from the raw laser speckle images captured by a CCD camera without scanning. We present a hardware-friendly algorithm for the real-time processing of laser speckle imaging. The algorithm is developed and optimized specifically for LSI processing in the field programmable gate array (FPGA). Based on this algorithm, we designed a dedicated hardware processor for real-time LSI in FPGA. The pipeline processing scheme and parallel computing architecture are introduced into the design of this LSI hardware processor. When the LSI hardware processor is implemented in the FPGA running at the maximum frequency of 130 MHz, up to 85 raw images with the resolution of 640×480 pixels can be processed per second. Meanwhile, we also present a system on chip (SOC) solution for LSI processing by integrating the CCD controller, memory controller, LSI hardware processor, and LCD display controller into a single FPGA chip. This SOC solution also can be used to produce an application specific integrated circuit for LSI processing.

High precision computing with charge domain devices and a pseudo-spectral method therefor

NASA Technical Reports Server (NTRS)

Barhen, Jacob (Inventor); Toomarian, Nikzad (Inventor); Fijany, Amir (Inventor); Zak, Michail (Inventor)

1997-01-01

The present invention enhances the bit resolution of a CCD/CID MVM processor by storing each bit of each matrix element as a separate CCD charge packet. The bits of each input vector are separately multiplied by each bit of each matrix element in massive parallelism and the resulting products are combined appropriately to synthesize the correct product. In another aspect of the invention, such arrays are employed in a pseudo-spectral method of the invention, in which partial differential equations are solved by expressing each derivative analytically as matrices, and the state function is updated at each computation cycle by multiplying it by the matrices. The matrices are treated as synaptic arrays of a neural network and the state function vector elements are treated as neurons. In a further aspect of the invention, moving target detection is performed by driving the soliton equation with a vector of detector outputs. The neural architecture consists of two synaptic arrays corresponding to the two differential terms of the soliton-equation and an adder connected to the output thereof and to the output of the detector array to drive the soliton equation.

Crystallization of the C-terminal domain of the addiction antidote CcdA in complex with its toxin CcdB

DOE Office of Scientific and Technical Information (OSTI.GOV)

Buts, Lieven; De Jonge, Natalie; Loris, Remy, E-mail: reloris@vub.ac.be

2005-10-01

The CcdA C-terminal domain was crystallized in complex with CcdB in two crystal forms that diffract to beyond 2.0 Å resolution. CcdA and CcdB are the antidote and toxin of the ccd addiction module of Escherichia coli plasmid F. The CcdA C-terminal domain (CcdA{sub C36}; 36 amino acids) was crystallized in complex with CcdB (dimer of 2 × 101 amino acids) in three different crystal forms, two of which diffract to high resolution. Form II belongs to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 37.6, b = 60.5, c = 83.8 Å and diffracts to 1.8more » Å resolution. Form III belongs to space group P2{sub 1}, with unit-cell parameters a = 41.0, b = 37.9, c = 69.6 Å, β = 96.9°, and diffracts to 1.9 Å resolution.« less

New Subarray Readout Patterns for the ACS Wide Field Channel

NASA Astrophysics Data System (ADS)

Golimowski, D.; Anderson, J.; Arslanian, S.; Chiaberge, M.; Grogin, N.; Lim, Pey Lian; Lupie, O.; McMaster, M.; Reinhart, M.; Schiffer, F.; Serrano, B.; Van Marshall, M.; Welty, A.

2017-04-01

At the start of Cycle 24, the original CCD-readout timing patterns used to generate ACS Wide Field Channel (WFC) subarray images were replaced with new patterns adapted from the four-quadrant readout pattern used to generate full-frame WFC images. The primary motivation for this replacement was a substantial reduction of observatory and staff resources needed to support WFC subarray bias calibration, which became a new and challenging obligation after the installation of the ACS CCD Electronics Box Replacement during Servicing Mission 4. The new readout patterns also improve the overall efficiency of observing with WFC subarrays and enable the processing of subarray images through stages of the ACS data calibration pipeline (calacs) that were previously restricted to full-frame WFC images. The new readout patterns replace the original 512×512, 1024×1024, and 2048×2046-pixel subarrays with subarrays having 2048 columns and 512, 1024, and 2048 rows, respectively. Whereas the original square subarrays were limited to certain WFC quadrants, the new rectangular subarrays are available in all four quadrants. The underlying bias structure of the new subarrays now conforms with those of the corresponding regions of the full-frame image, which allows raw frames in all image formats to be calibrated using one contemporaneous full-frame "superbias" reference image. The original subarrays remain available for scientific use, but calibration of these image formats is no longer supported by STScI.

Parallel, confocal, and complete spectrum imager for fluorescent detection of high-density microarray

NASA Astrophysics Data System (ADS)

Bogdanov, Valery L.; Boyce-Jacino, Michael

1999-05-01

Confined arrays of biochemical probes deposited on a solid support surface (analytical microarray or 'chip') provide an opportunity to analysis multiple reactions simultaneously. Microarrays are increasingly used in genetics, medicine and environment scanning as research and analytical instruments. A power of microarray technology comes from its parallelism which grows with array miniaturization, minimization of reagent volume per reaction site and reaction multiplexing. An optical detector of microarray signals should combine high sensitivity, spatial and spectral resolution. Additionally, low-cost and a high processing rate are needed to transfer microarray technology into biomedical practice. We designed an imager that provides confocal and complete spectrum detection of entire fluorescently-labeled microarray in parallel. Imager uses microlens array, non-slit spectral decomposer, and high- sensitive detector (cooled CCD). Two imaging channels provide a simultaneous detection of localization, integrated and spectral intensities for each reaction site in microarray. A dimensional matching between microarray and imager's optics eliminates all in moving parts in instrumentation, enabling highly informative, fast and low-cost microarray detection. We report theory of confocal hyperspectral imaging with microlenses array and experimental data for implementation of developed imager to detect fluorescently labeled microarray with a density approximately 103 sites per cm2.

Development of radiation tolerant monolithic active pixel sensors with fast column parallel read-out

NASA Astrophysics Data System (ADS)

Koziel, M.; Dorokhov, A.; Fontaine, J.-C.; De Masi, R.; Winter, M.

2010-12-01

Monolithic active pixel sensors (MAPS) [1] (Turchetta et al., 2001) are being developed at IPHC—Strasbourg to equip the EUDET telescope [2] (Haas, 2006) and vertex detectors for future high energy physics experiments, including the STAR upgrade at RHIC [3] (T.S. Collaboration, 2005) and the CBM experiment at FAIR/GSI [4] (Heuser, 2006). High granularity, low material budget and high read-out speed are systematically required for most applications, complemented, for some of them, with high radiation tolerance. A specific column-parallel architecture, implemented in the MIMOSA-22 sensor, was developed to achieve fast read-out MAPS. Previous studies of the front-end architecture integrated in this sensor, which includes in-pixel amplification, have shown that the fixed pattern noise increase consecutive to ionizing radiation can be controlled by means of a negative feedback [5] (Hu-Guo et al., 2008). However, an unexpected rise of the temporal noise was observed. A second version of this chip (MIMOSA-22bis) was produced in order to search for possible improvements of the radiation tolerance, regarding this type of noise. In this prototype, the feedback transistor was tuned in order to mitigate the sensitivity of the pixel to ionizing radiation. The performances of the pixels after irradiation were investigated for two types of feedback transistors: enclosed layout transistor (ELT) [6] (Snoeys et al., 2000) and "standard" transistor with either large or small transconductance. The noise performance of all test structures was studied in various conditions (expected in future experiments) regarding temperature, integration time and ionizing radiation dose. Test results are presented in this paper. Based on these observations, ideas for further improvement of the radiation tolerance of column parallel MAPS are derived.

Linear CCD attitude measurement system based on the identification of the auxiliary array CCD

NASA Astrophysics Data System (ADS)

Hu, Yinghui; Yuan, Feng; Li, Kai; Wang, Yan

2015-10-01

Object to the high precision flying target attitude measurement issues of a large space and large field of view, comparing existing measurement methods, the idea is proposed of using two array CCD to assist in identifying the three linear CCD with multi-cooperative target attitude measurement system, and to address the existing nonlinear system errors and calibration parameters and more problems with nine linear CCD spectroscopic test system of too complicated constraints among camera position caused by excessive. The mathematical model of binocular vision and three linear CCD test system are established, co-spot composition triangle utilize three red LED position light, three points' coordinates are given in advance by Cooperate Measuring Machine, the red LED in the composition of the three sides of a triangle adds three blue LED light points as an auxiliary, so that array CCD is easier to identify three red LED light points, and linear CCD camera is installed of a red filter to filter out the blue LED light points while reducing stray light. Using array CCD to measure the spot, identifying and calculating the spatial coordinates solutions of red LED light points, while utilizing linear CCD to measure three red LED spot for solving linear CCD test system, which can be drawn from 27 solution. Measured with array CCD coordinates auxiliary linear CCD has achieved spot identification, and has solved the difficult problems of multi-objective linear CCD identification. Unique combination of linear CCD imaging features, linear CCD special cylindrical lens system is developed using telecentric optical design, the energy center of the spot position in the depth range of convergence in the direction is perpendicular to the optical axis of the small changes ensuring highprecision image quality, and the entire test system improves spatial object attitude measurement speed and precision.

A temperature controller board for the ARC controller

NASA Astrophysics Data System (ADS)

Tulloch, Simon

2016-07-01

A high-performance temperature controller board has been produced for the ARC Generation-3 CCD controller. It contains two 9W temperature servo loops and four temperature input channels and is fully programmable via the ARC API and OWL data acquisition program. PI-loop control is implemented in an on-board micro. Both diode and RTD sensors can be used. Control and telemetry data is sent via the ARC backplane although a USB-2 interface is also available. Further functionality includes hardware timers and high current drivers for external shutters and calibration LEDs, an LCD display, a parallel i/o port, a pressure sensor interface and an uncommitted analogue telemetry input.

Structure of the runaway electron loss during induced disruptions in TEXTOR

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wongrach, K.; Finken, K. H.; Willi, O.

2015-10-15

The loss of runaway electrons during an induced disruption is recorded by a synchrotron imaging technique using a fast infrared CCD camera. The loss is predominantly diffuse. During the “spiky-loss phase”, when the runaway beam moves close to the wall, a narrow channel between the runaway column and a scintillator probe is formed and lasts until the runaway beam is terminated. In some cases, the processed images show a stripe pattern at the plasma edge. A comparison between the MHD dominated disruptions and the MHD-free disruption is performed. A new mechanism of plasma disruptions with the runaway electron generation andmore » a novel model which reproduces many characteristic features of the plasma beam evolution during a disruption is briefly described.« less

Trichlorido[(meth­yl{2-[meth­yl(2-pyridyl­meth­yl)amino]eth­yl}amino)acetonitrile]iron(III) methanol hemisolvate

PubMed Central

Nielsen, Anne; McKenzie, Christine J.; Bond, Andrew D.

2009-01-01

The title compound, [FeCl3(C12H18N4)]·0.5CH3OH, contains an FeIII ion in a distorted octa­hedral coordination environment. The neutral N,N′,N′′-tridentate ligand adopts a fac coordination mode, and chloride ligands lie trans to each of the three coordinated N atoms. In the crystal, the complexes form columns extending parallel to the approximate local threefold axes of the FeN3Cl3 octa­hedra, and the columns are arranged so that the uncoordinated nitrile groups align in an anti­parallel manner and the pyridyl rings form offset face-to-face arrangements [inter­planar separations = 2.95 (1) and 3.11 (1) Å; centroid–centroid distances = 5.31 (1) and 4.92 (1) Å]. The methanol solvent mol­ecule is disordered about a twofold rotation axis. PMID:21578169

Solar array construction

NASA Technical Reports Server (NTRS)

Crouthamel, Marvin S. (Inventor); Coyle, Peter J. (Inventor)

1982-01-01

An interconnect tab on each cell of a first set of circular solar cells connects that cell in series with an adjacent cell in the set. This set of cells is arranged in alternate columns and rows of an array and a second set of similar cells is arranged in the remaining alternate columns and rows of the array. Three interconnect tabs on each solar cell of the said second set are employed to connect the cells of the second set to one another, in series and to connect the cells of the second set to those of the first set in parallel. Some tabs (making parallel connections) connect the same surface regions of adjacent cells to one another and others (making series connections) connect a surface region of one cell to the opposite surface region of an adjacent cell; however, the tabs are so positioned that the array may be easily assembled by depositing the cells in a certain sequence and in proper orientation.

Back-illuminate fiber system research for multi-object fiber spectroscopic telescope

NASA Astrophysics Data System (ADS)

Zhou, Zengxiang; Liu, Zhigang; Hu, Hongzhuan; Wang, Jianping; Zhai, Chao; Chu, Jiaru

2016-07-01

In the telescope observation, the position of fiber will highly influence the spectra efficient input in the fiber to the spectrograph. When the fibers were back illuminated on the spectra end, they would export light on the positioner end, so the CCD cameras could capture the photo of fiber tip position covered the focal plane, calculates the precise position information by light centroid method and feeds back to control system. A set of fiber back illuminated system was developed which combined to the low revolution spectro instruments in LAMOST. It could provide uniform light output to the fibers, meet the requirements for the CCD camera measurement. The paper was introduced the back illuminated system design and different test for the light resource. After optimization, the effect illuminated system could compare with the integrating sphere, meet the conditions of fiber position measurement.Using parallel controlled fiber positioner as the spectroscopic receiver is an efficiency observation system for spectra survey, has been used in LAMOST recently, and will be proposed in CFHT and rebuilt telescope Mayall. In the telescope observation, the position of fiber will highly influence the spectra efficient input in the fiber to the spectrograph. When the fibers were back illuminated on the spectra end, they would export light on the positioner end, so the CCD cameras could capture the photo of fiber tip position covered the focal plane, calculates the precise position information by light centroid method and feeds back to control system. After many years on these research, the back illuminated fiber measurement was the best method to acquire the precision position of fibers. In LAMOST, a set of fiber back illuminated system was developed which combined to the low revolution spectro instruments in LAMOST. It could provide uniform light output to the fibers, meet the requirements for the CCD camera measurement and was controlled by high-level observation system which could shut down during the telescope observation. The paper was introduced the back illuminated system design and different test for the light resource. After optimization, the effect illuminated system could compare the integrating sphere, meet the conditions of fiber position measurement.

Technical Note: Range verification system using edge detection method for a scintillator and a CCD camera system.

PubMed

Saotome, Naoya; Furukawa, Takuji; Hara, Yousuke; Mizushima, Kota; Tansho, Ryohei; Saraya, Yuichi; Shirai, Toshiyuki; Noda, Koji

2016-04-01

Three-dimensional irradiation with a scanned carbon-ion beam has been performed from 2011 at the authors' facility. The authors have developed the rotating-gantry equipped with the scanning irradiation system. The number of combinations of beam properties to measure for the commissioning is more than 7200, i.e., 201 energy steps, 3 intensities, and 12 gantry angles. To compress the commissioning time, quick and simple range verification system is required. In this work, the authors develop a quick range verification system using scintillator and charge-coupled device (CCD) camera and estimate the accuracy of the range verification. A cylindrical plastic scintillator block and a CCD camera were installed on the black box. The optical spatial resolution of the system is 0.2 mm/pixel. The camera control system was connected and communicates with the measurement system that is part of the scanning system. The range was determined by image processing. Reference range for each energy beam was determined by a difference of Gaussian (DOG) method and the 80% of distal dose of the depth-dose distribution that were measured by a large parallel-plate ionization chamber. The authors compared a threshold method and a DOG method. The authors found that the edge detection method (i.e., the DOG method) is best for the range detection. The accuracy of range detection using this system is within 0.2 mm, and the reproducibility of the same energy measurement is within 0.1 mm without setup error. The results of this study demonstrate that the authors' range check system is capable of quick and easy range verification with sufficient accuracy.

Technical Note: Range verification system using edge detection method for a scintillator and a CCD camera system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Saotome, Naoya, E-mail: naosao@nirs.go.jp; Furukawa, Takuji; Hara, Yousuke

Purpose: Three-dimensional irradiation with a scanned carbon-ion beam has been performed from 2011 at the authors’ facility. The authors have developed the rotating-gantry equipped with the scanning irradiation system. The number of combinations of beam properties to measure for the commissioning is more than 7200, i.e., 201 energy steps, 3 intensities, and 12 gantry angles. To compress the commissioning time, quick and simple range verification system is required. In this work, the authors develop a quick range verification system using scintillator and charge-coupled device (CCD) camera and estimate the accuracy of the range verification. Methods: A cylindrical plastic scintillator blockmore » and a CCD camera were installed on the black box. The optical spatial resolution of the system is 0.2 mm/pixel. The camera control system was connected and communicates with the measurement system that is part of the scanning system. The range was determined by image processing. Reference range for each energy beam was determined by a difference of Gaussian (DOG) method and the 80% of distal dose of the depth-dose distribution that were measured by a large parallel-plate ionization chamber. The authors compared a threshold method and a DOG method. Results: The authors found that the edge detection method (i.e., the DOG method) is best for the range detection. The accuracy of range detection using this system is within 0.2 mm, and the reproducibility of the same energy measurement is within 0.1 mm without setup error. Conclusions: The results of this study demonstrate that the authors’ range check system is capable of quick and easy range verification with sufficient accuracy.« less

Functional characterisation of three members of the Vitis vinifera L. carotenoid cleavage dioxygenase gene family

PubMed Central

2013-01-01

Background In plants, carotenoids serve as the precursors to C13-norisoprenoids, a group of apocarotenoid compounds with diverse biological functions. Enzymatic cleavage of carotenoids catalysed by members of the carotenoid cleavage dioxygenase (CCD) family has been shown to produce a number of industrially important volatile flavour and aroma apocarotenoids including β-ionone, geranylacetone, pseudoionone, α-ionone and 3-hydroxy-β-ionone in a range of plant species. Apocarotenoids contribute to the floral and fruity attributes of many wine cultivars and are thereby, at least partly, responsible for the “varietal character”. Despite their importance in grapes and wine; carotenoid cleavage activity has only been described for VvCCD1 and the mechanism(s) and regulation of carotenoid catabolism remains largely unknown. Results Three grapevine-derived CCD-encoding genes have been isolated and shown to be functional with unique substrate cleavage capacities. Our results demonstrate that the VvCCD4a and VvCCD4b catalyse the cleavage of both linear and cyclic carotenoid substrates. The expression of VvCCD1, VvCCD4a and VvCCD4b was detected in leaf, flower and throughout berry development. VvCCD1 expression was constitutive, whereas VvCCD4a expression was predominant in leaves and VvCCD4b in berries. A transgenic population with a 12-fold range of VvCCD1 expression exhibited a lack of correlation between VvCCD1 expression and carotenoid substrates and/or apocarotenoid products in leaves, providing proof that the in planta function(s) of VvCCD1 in photosynthetically active tissue is distinct from the in vitro activities demonstrated. The isolation and functional characterisation of VvCCD4a and VvCCD4b identify two additional CCDs that are functional in grapevine. Conclusions Taken together, our results indicate that the three CCDs are under various levels of control that include gene expression (spatial and temporal), substrate specificity and compartmentalisation that act individually and/or co-ordinately to maintain carotenoid and volatile apocarotenoid levels in plants. Altering the expression of VvCCD1 in a transgenic grapevine population illustrated the divergence between the in vitro enzyme activity and the in planta activity of this enzyme, thereby contributing to the efforts to understand how enzymatic degradation of carotenoids involved in photosynthesis occurs. The identification and functional characterisation of VvCCD4a and VvCCD4b suggest that these enzymes are primarily responsible for catalysing the cleavage of plastidial carotenoids. PMID:24106789

Simultaneous liquid chromatography/mass spectrometry determination of both polar and "multiresidue" pesticides in food using parallel hydrophilic interaction/reversed-phase liquid chromatography and a hybrid sample preparation approach.

PubMed

Robles-Molina, José; Gilbert-López, Bienvenida; García-Reyes, Juan F; Molina-Díaz, Antonio

2017-09-29

Pesticide testing of foodstuffs is usually accomplished with generic wide-scope multi-residue methods based on liquid chromatography tandem mass spectrometry (LC-MS/MS). However, this approach does not cover some special pesticides, the so called "single-residue method" compounds, that are hardly compatible with standard reversed-phase (RP) separations due to their specific properties. In this article, we propose a comprehensive strategy for the integration of single residue method compounds and standard multiresidue pesticides within a single run. It is based on the use of a parallel LC column assembly with two different LC gradients performing orthogonal hydrophilic interaction chromatography (HILIC) and reversed-phase (RPLC) chromatography within one analytical run. Two sample aliquots were simultaneously injected on each column, using different gradients, being the eluents merged post-column prior to mass spectrometry detection. The approach was tested with 41 multiclass pesticides covering a wide range of physicochemical properties across several orders of log K ow (from -4 to +5.5). With this assembly, distinct separation from the void was attained for all the pesticides studied, keeping similar performance in terms of sensitivity, peak area reproducibility (<6 RSD% in most cases) and retention time stability of standard single column approaches (better than±0.1min). The application of the proposed approach using parallel HILIC/RPLC and RPLC/aqueous normal phase (Obelisc) were assessed in leek using LC-MS/MS. For this purpose, a hybrid QuEChERS (Quick, easy, cheap, effective, rugged and safe)/QuPPe (quick method for polar pesticides) method was evaluated based on solvent extraction with MeOH and acetonitrile followed by dispersive solid-phase extraction, delivering appropriate recoveries for most of the pesticides included in the study within the log K ow in the range from -4 to +5.5. The proposed strategy may be extended to other fields such as sport drug testing or environmental analysis, where the same type of variety of analytes featuring poor retention within a single chromatographic separation occurs. Copyright © 2017 Elsevier B.V. All rights reserved.

A picoliter-volume mixer for microfluidic analytical systems.

PubMed

He, B; Burke, B J; Zhang, X; Zhang, R; Regnier, F E

2001-05-01

Mixing confluent liquid streams is an important, but difficult operation in microfluidic systems. This paper reports the construction and characterization of a 100-pL mixer for liquids transported by electroosmotic flow. Mixing was achieved in a microfabricated device with multiple intersecting channels of varying lengths and a bimodal width distribution. All channels running parallel to the direction of flow were 5 microm in width whereas larger 27-microm-width channels ran back and forth through the parallel channel network at a 45 degrees angle. The channel network composing the mixer was approximately 10 microm deep. It was observed that little mixing of the confluent solvent streams occurred in the 100-microm-wide, 300-microm-long mixer inlet channel where mixing would be achieved almost exclusively by diffusion. In contrast, after passage through the channel network in the approximately 200-microm-length static mixer bed, mixing was complete as determined by confocal microscopy and CCD detection. Theoretical simulations were also performed in an attempt to describe the extent of mixing in microfabricated systems.

Derringer desirability and kinetic plot LC-column comparison approach for MS-compatible lipopeptide analysis.

PubMed

D'Hondt, Matthias; Verbeke, Frederick; Stalmans, Sofie; Gevaert, Bert; Wynendaele, Evelien; De Spiegeleer, Bart

2014-06-01

Lipopeptides are currently re-emerging as an interesting subgroup in the peptide research field, having historical applications as antibacterial and antifungal agents and new potential applications as antiviral, antitumor, immune-modulating and cell-penetrating compounds. However, due to their specific structure, chromatographic analysis often requires special buffer systems or the use of trifluoroacetic acid, limiting mass spectrometry detection. Therefore, we used a traditional aqueous/acetonitrile based gradient system, containing 0.1% (m/v) formic acid, to separate four pharmaceutically relevant lipopeptides (polymyxin B 1 , caspofungin, daptomycin and gramicidin A 1 ), which were selected based upon hierarchical cluster analysis (HCA) and principal component analysis (PCA). In total, the performance of four different C18 columns, including one UPLC column, were evaluated using two parallel approaches. First, a Derringer desirability function was used, whereby six single and multiple chromatographic response values were rescaled into one overall D -value per column. Using this approach, the YMC Pack Pro C18 column was ranked as the best column for general MS-compatible lipopeptide separation. Secondly, the kinetic plot approach was used to compare the different columns at different flow rate ranges. As the optimal kinetic column performance is obtained at its maximal pressure, the length elongation factor λ ( P max / P exp ) was used to transform the obtained experimental data (retention times and peak capacities) and construct kinetic performance limit (KPL) curves, allowing a direct visual and unbiased comparison of the selected columns, whereby the YMC Triart C18 UPLC and ACE C18 columns performed as best. Finally, differences in column performance and the (dis)advantages of both approaches are discussed.

Fast, high-resolution 3D dosimetry utilizing a novel optical-CT scanner incorporating tertiary telecentric collimation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sakhalkar, H. S.; Oldham, M.

2008-01-15

This study introduces a charge coupled device (CCD) area detector based optical-computed tomography (optical-CT) scanner for comprehensive verification of radiation dose distributions recorded in nonscattering radiochromic dosimeters. Defining characteristics include: (i) a very fast scanning time of {approx}5 min to acquire a complete three-dimensional (3D) dataset, (ii) improved image formation through the use of custom telecentric optics, which ensures accurate projection images and minimizes artifacts from scattered and stray-light sources, and (iii) high resolution (potentially 50 {mu}m) isotropic 3D dose readout. The performance of the CCD scanner for 3D dose readout was evaluated by comparison with independent 3D readout frommore » the single laser beam OCTOPUS-scanner for the same PRESAGE dosimeters. The OCTOPUS scanner was considered the 'gold standard' technique in light of prior studies demonstrating its accuracy. Additional comparisons were made against calculated dose distributions from the ECLIPSE treatment-planning system. Dose readout for the following treatments were investigated: (i) a single rectangular beam irradiation to investigate small field and very steep dose gradient dosimetry away from edge effects, (ii) a 2-field open beam parallel-opposed irradiation to investigate dosimetry along steep dose gradients, and (iii) a 7-field intensity modulated radiation therapy (IMRT) irradiation to investigate dosimetry for complex treatment delivery involving modulation of fluence and for dosimetry along moderate dose gradients. Dose profiles, dose-difference plots, and gamma maps were employed to evaluate quantitative estimates of agreement between independently measured and calculated dose distributions. Results indicated that dose readout from the CCD scanner was in agreement with independent gold-standard readout from the OCTOPUS-scanner as well as the calculated ECLIPSE dose distribution for all treatments, except in regions within a few millimeters of the edge of the dosimeter, where edge artifact is predominant. Agreement of line profiles was observed, even along steep dose gradients. Dose difference plots indicated that the CCD scanner dose readout differed from the OCTOPUSscanner readout and ECLIPSE calculations by {approx}10% along steep dose gradients and by {approx}5% along moderate dose gradients. Gamma maps (3% dose-difference and 3 mm distance-to-agreement acceptance criteria) revealed agreement, except for regions within 5 mm of the edge of the dosimeter where the edge artifact occurs. In summary, the data demonstrate feasibility of using the fast, high-resolution CCD scanner for comprehensive 3D dosimetry in all applications, except where dose readout is required close to the edges of the dosimeter. Further work is ongoing to reduce this artifact.« less

Fine structure of Galactic foreground ISM towards high-redshift AGN - utilizing Herschel PACS and SPIRE data

NASA Astrophysics Data System (ADS)

Perger, K.; Pinter, S.; Frey, S.; Tóth, L. V.

2018-05-01

One of the most certain ways to determine star formation rate in galaxies is based on far infrared (FIR) measurements. To decide the origin of the observed FIR emission, subtracting the Galactic foreground is a crucial step. We utilized Herschel photometric data to determine the hydrogen column densities in three galactic latitude regions, at b = 27°, 50° and -80°. We applied a pixel-by-pixel fit to the spectral energy distribution (SED) for the images aquired from parallel PACS-SPIRE observations in all three sky areas. We determined the column densities with resolutions 45'' and 6', and compared the results with values estimated from the IRAS dust maps. Column densities at 27° and 50° galactic latitudes determined from the Herschel data are in a good agreement with the literature values. However, at the highest galactic latitude we found that the column densities from the Herschel data exceed those derived from the IRAS dust map.

SU-G-BRB-09: Kompeito-Shot: Development of a Novel Verification System for 3D Beam Alignment Including the Sag of Gantry Head

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tsuneda, M; Nishio, T; Saito, A

Purpose: High accuracy of beam axis is required for high-precision radiation therapy. It is impossible to quantitatively and directly evaluate the sagging effect of the gantry head using current methods (star-shot and Winston-Lutz tests) when the gantry head sags under the weight of MLC and X-Y jaws. We introduce a novel method “Kompeito-shot (3D star-shot)” for the verification of 3D beam alignment (3D isocentricity). This method enables direct measurement of the sagging effect. We developed the system and examined the concept of this system. Methods: The system composed of a plastic scintillator (PS), a truncated cone-shaped mirror, a plane mirrormore » and a CCD camera. Two types of PS were compared. One consisted of a column PS (Co system), the other consisted of a column PS inserted into a barrel PS with shading film in between (Co-Ba system). The system was irradiated with a 6-MV photon beam and the scintillation light was measured using the CCD camera through the mirror system. The gantry angle was set from 270 to 300 degrees to mimic the sagging of the gantry head for evaluating the accuracy of the system. The distance between a center of PS and entrance / exit points were calculated to analyze the gantry angle. And, the calculated gantry angle and the irradiated gantry angle were compared. Results: We compared the measured image of Co system and that of Co-Ba system. Entrance and exit areas were visualized clearly. The histogram showing the difference between the calculated gantry angle and the irradiated gantry angle was fitted with a Gaussian function. Mean and standard deviation of Co-Ba system were smaller than that of Co system by one order of magnitude. Conclusion: We developed the Kompeito-shot system and evaluated the accuracy of the system. The basic concept works for the verification of 3D isocentricity.« less

Jig Aligns Shadow Mask On CCD

NASA Technical Reports Server (NTRS)

Matus, Carlos V.

1989-01-01

Alignment viewed through microscope. Alignment jig positions shadow mask on charge-coupled device (CCD) so metal film deposited on it precisely. Allows CCD package to be inserted and removed without disturbing alignment of mask. Holds CCD packages securely and isolates it electrostatically while providing electrical contact to each of its pins. When alignment jig assembled with CCD, used to move mask under micrometer control.

Cytosolic and Plastoglobule-targeted Carotenoid Dioxygenases from Crocus sativus Are Both Involved in β-Ionone Release*

PubMed Central

Rubio, Angela; Rambla, José Luís; Santaella, Marcella; Gómez, M. Dolores; Orzaez, Diego; Granell, Antonio; Gómez-Gómez, Lourdes

2008-01-01

Saffron, the processed stigma of Crocus sativus, is characterized by the presence of several apocarotenoids that contribute to the color, flavor, and aroma of the spice. However, little is known about the synthesis of aroma compounds during the development of the C. sativus stigma. The developing stigma is nearly odorless, but before and at anthesis, the aromatic compound β-ionone becomes the principal norisoprenoid volatile in the stigma. In this study, four carotenoid cleavage dioxygenase (CCD) genes, CsCCD1a, CsCCD1b, CsCCD4a, and CsCCD4b, were isolated from C. sativus. Expression analysis showed that CsCCD1a was constitutively expressed, CsCCD1b was unique to the stigma tissue, but only CsCCD4a and -b had expression patterns consistent with the highest levels of β-carotene and emission of β-ionone derived during the stigma development. The CsCCD4 enzymes were localized in plastids and more specifically were present in the plastoglobules. The enzymatic activities of CsCCD1a, CsCCD1b, and CsCCD4 enzymes were determined by Escherichia coli expression, and subsequent analysis of the volatile products was generated by GC/MS. The four CCDs fell in two phylogenetically divergent dioxygenase classes, but all could cleave β-carotene at the 9,10(9′,10′) positions to yield β-ionone. The data obtained suggest that all four C. sativus CCD enzymes may contribute in different ways to the production of β-ionone. In addition, the location and precise timing of β-ionone synthesis, together with its known activity as a fragrance and insect attractant, suggest that this volatile may have a role in Crocus pollination. PMID:18611853

Using a delta-doped CCD to determine the energy of a low-energy particle

NASA Technical Reports Server (NTRS)

Nikzad, Shouleh (Inventor); Croley, Donald R. (Inventor); Murphy, Gerald B. (Inventor)

2001-01-01

The back surface of a thinned charged-coupled device (CCD) is treated to eliminate the backside potential well that appears in a conventional thinned CCD during backside illumination. The backside of the CCD includes a delta layer of high-concentration dopant confined to less than one monolayer of the crystal semiconductor. The thinned, delta-doped CCD is used to determine the energy of a very low-energy particle that penetrates less than 1.0 nm into the CCD, such as a proton having energy less than 10 keV.

Delta-doped CCD's as low-energy particle detectors and imagers

NASA Technical Reports Server (NTRS)

Nikzad, Shouleh (Inventor); Hoenk, Michael E. (Inventor); Hecht, Michael H. (Inventor)

2002-01-01

The back surface of a thinned charged-coupled device (CCD) is treated to eliminate the backside potential well that appears in a conventional thinned CCD during backside illumination. The backside of the CCD includes a delta layer of high-concentration dopant confined to less than one monolayer of the crystal semiconductor. The thinned, delta-doped CCD is used to detect very low-energy particles that penetrate less than 1.0 nm into the CCD, including electrons having energies less than 1000 eV and protons having energies less than 10 keV.

A strand-passage conformation of DNA gyrase is required to allow the bacterial toxin, CcdB, to access its binding site

PubMed Central

Smith, Andrew B.; Maxwell, Anthony

2006-01-01

DNA gyrase is the only topoisomerase able to introduce negative supercoils into DNA. Absent in humans, gyrase is a successful target for antibacterial drugs. However, increasing drug resistance is a serious problem and new agents are urgently needed. The naturally-produced Escherichia coli toxin CcdB has been shown to target gyrase by what is predicted to be a novel mechanism. CcdB has been previously shown to stabilize the gyrase ‘cleavage complex’, but it has not been shown to inhibit the catalytic reactions of gyrase. We present data showing that CcdB does indeed inhibit the catalytic reactions of gyrase by stabilization of the cleavage complex and that the GyrA C-terminal DNA-wrapping domain and the GyrB N-terminal ATPase domain are dispensable for CcdB's action. We further investigate the role of specific GyrA residues in the action of CcdB by site-directed mutagenesis; these data corroborate a model for CcdB action based on a recent crystal structure of a CcdB–GyrA fragment complex. From this work, we are now able to present a model for CcdB action that explains all previous observations relating to CcdB–gyrase interaction. CcdB action requires a conformation of gyrase that is only revealed when DNA strand passage is taking place. PMID:16963775

A strand-passage conformation of DNA gyrase is required to allow the bacterial toxin, CcdB, to access its binding site.

PubMed

Smith, Andrew B; Maxwell, Anthony

2006-01-01

DNA gyrase is the only topoisomerase able to introduce negative supercoils into DNA. Absent in humans, gyrase is a successful target for antibacterial drugs. However, increasing drug resistance is a serious problem and new agents are urgently needed. The naturally-produced Escherichia coli toxin CcdB has been shown to target gyrase by what is predicted to be a novel mechanism. CcdB has been previously shown to stabilize the gyrase 'cleavage complex', but it has not been shown to inhibit the catalytic reactions of gyrase. We present data showing that CcdB does indeed inhibit the catalytic reactions of gyrase by stabilization of the cleavage complex and that the GyrA C-terminal DNA-wrapping domain and the GyrB N-terminal ATPase domain are dispensable for CcdB's action. We further investigate the role of specific GyrA residues in the action of CcdB by site-directed mutagenesis; these data corroborate a model for CcdB action based on a recent crystal structure of a CcdB-GyrA fragment complex. From this work, we are now able to present a model for CcdB action that explains all previous observations relating to CcdB-gyrase interaction. CcdB action requires a conformation of gyrase that is only revealed when DNA strand passage is taking place.

Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE7 is involved in the production of negative and positive branching signals in petunia.

PubMed

Drummond, Revel S M; Martínez-Sánchez, N Marcela; Janssen, Bart J; Templeton, Kerry R; Simons, Joanne L; Quinn, Brian D; Karunairetnam, Sakuntala; Snowden, Kimberley C

2009-12-01

One of the key factors that defines plant form is the regulation of when and where branches develop. The diversity of form observed in nature results, in part, from variation in the regulation of branching between species. Two CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes, CCD7 and CCD8, are required for the production of a branch-suppressing plant hormone. Here, we report that the decreased apical dominance3 (dad3) mutant of petunia (Petunia hybrida) results from the mutation of the PhCCD7 gene and has a less severe branching phenotype than mutation of PhCCD8 (dad1). An analysis of the expression of this gene in wild-type, mutant, and grafted petunia suggests that in petunia, CCD7 and CCD8 are coordinately regulated. In contrast to observations in Arabidopsis (Arabidopsis thaliana), ccd7ccd8 double mutants in petunia show an additive phenotype. An analysis using dad3 or dad1 mutant scions grafted to wild-type rootstocks showed that when these plants produce adventitious mutant roots, branching is increased above that seen in plants where the mutant roots are removed. The results presented here indicate that mutation of either CCD7 or CCD8 in petunia results in both the loss of an inhibitor of branching and an increase in a promoter of branching.

Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE7 Is Involved in the Production of Negative and Positive Branching Signals in Petunia1[W][OA

PubMed Central

Drummond, Revel S.M.; Martínez-Sánchez, N. Marcela; Janssen, Bart J.; Templeton, Kerry R.; Simons, Joanne L.; Quinn, Brian D.; Karunairetnam, Sakuntala; Snowden, Kimberley C.

2009-01-01

One of the key factors that defines plant form is the regulation of when and where branches develop. The diversity of form observed in nature results, in part, from variation in the regulation of branching between species. Two CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes, CCD7 and CCD8, are required for the production of a branch-suppressing plant hormone. Here, we report that the decreased apical dominance3 (dad3) mutant of petunia (Petunia hybrida) results from the mutation of the PhCCD7 gene and has a less severe branching phenotype than mutation of PhCCD8 (dad1). An analysis of the expression of this gene in wild-type, mutant, and grafted petunia suggests that in petunia, CCD7 and CCD8 are coordinately regulated. In contrast to observations in Arabidopsis (Arabidopsis thaliana), ccd7ccd8 double mutants in petunia show an additive phenotype. An analysis using dad3 or dad1 mutant scions grafted to wild-type rootstocks showed that when these plants produce adventitious mutant roots, branching is increased above that seen in plants where the mutant roots are removed. The results presented here indicate that mutation of either CCD7 or CCD8 in petunia results in both the loss of an inhibitor of branching and an increase in a promoter of branching. PMID:19846541

Efficiency gain limits of the parallel segmented inlet and outlet flow concept in analytical liquid chromatography columns suffering from radial transcolumn packing density gradients.

PubMed

Broeckhoven, Ken; Desmet, Gert

2012-10-05

The maximal gain in efficiency that can be expected from the use of the segmented column end fittings that were recently introduced to alleviate the effect of transcolumn packing density gradients has been quantified and generalized using numerical computations of the band broadening process. It was found that, for an unretained compound in a column with a parabolic packing density gradient, the use of a segmented inlet or a segmented outlet allows to eliminate about 60-100% of the plate height contribution (H(tc)) originating from a parabolic transcolumn velocity gradient in a d(c)=4.6 mm column. In a d(c)=2.1 mm column, these percentages change from 10 to 100%. Using a combined segmented in- and outlet, H(tc) can be reduced by about 90-100% (d(c)=4.6 mm column) or 20-100% (d(c)=2.1 mm column). The strong variation of these gain percentages is due to fact that they depend very strongly on the column length and the flow rate. Dimensionless graphs have been established that allow to directly quantify the effect for each specific case. It was also found that, in agreement with one's physical intuition, trans-column velocity profiles that are more flat in the central region benefit more from the concept than sharp, parabolic-like profiles. The gain margins furthermore tend to become smaller with increasing retention and increasing diffusion coefficient. Copyright © 2012 Elsevier B.V. All rights reserved.

Improved Scanners for Microscopic Hyperspectral Imaging

NASA Technical Reports Server (NTRS)

Mao, Chengye

2009-01-01

Improved scanners to be incorporated into hyperspectral microscope-based imaging systems have been invented. Heretofore, in microscopic imaging, including spectral imaging, it has been customary to either move the specimen relative to the optical assembly that includes the microscope or else move the entire assembly relative to the specimen. It becomes extremely difficult to control such scanning when submicron translation increments are required, because the high magnification of the microscope enlarges all movements in the specimen image on the focal plane. To overcome this difficulty, in a system based on this invention, no attempt would be made to move either the specimen or the optical assembly. Instead, an objective lens would be moved within the assembly so as to cause translation of the image at the focal plane: the effect would be equivalent to scanning in the focal plane. The upper part of the figure depicts a generic proposed microscope-based hyperspectral imaging system incorporating the invention. The optical assembly of this system would include an objective lens (normally, a microscope objective lens) and a charge-coupled-device (CCD) camera. The objective lens would be mounted on a servomotor-driven translation stage, which would be capable of moving the lens in precisely controlled increments, relative to the camera, parallel to the focal-plane scan axis. The output of the CCD camera would be digitized and fed to a frame grabber in a computer. The computer would store the frame-grabber output for subsequent viewing and/or processing of images. The computer would contain a position-control interface board, through which it would control the servomotor. There are several versions of the invention. An essential feature common to all versions is that the stationary optical subassembly containing the camera would also contain a spatial window, at the focal plane of the objective lens, that would pass only a selected portion of the image. In one version, the window would be a slit, the CCD would contain a one-dimensional array of pixels, and the objective lens would be moved along an axis perpendicular to the slit to spatially scan the image of the specimen in pushbroom fashion. The image built up by scanning in this case would be an ordinary (non-spectral) image. In another version, the optics of which are depicted in the lower part of the figure, the spatial window would be a slit, the CCD would contain a two-dimensional array of pixels, the slit image would be refocused onto the CCD by a relay-lens pair consisting of a collimating and a focusing lens, and a prism-gratingprism optical spectrometer would be placed between the collimating and focusing lenses. Consequently, the image on the CCD would be spatially resolved along the slit axis and spectrally resolved along the axis perpendicular to the slit. As in the first-mentioned version, the objective lens would be moved along an axis perpendicular to the slit to spatially scan the image of the specimen in pushbroom fashion.

Electropneumatic rheostat regulates high current

NASA Technical Reports Server (NTRS)

Haacker, J. F.; Jedlicka, J. R.; Wagoner, C. B.

1965-01-01

Electropneumatic rheostat maintains a constant direct current in each of several high-power parallel loads, of variable resistance, across a single source. It provides current regulation at any preset value by dissipating the proper amount of energy thermally, and uses a column of mercury to vary the effective length of a resistance element.

Glossary of Fire Terms

Science.gov Websites

a slope faces. Backfiring When attacking a wildland fire using the indirect attack method convective column. Black Line When putting in control lines, the process of burning out any pockets of small wildland fire. Burning Out When attack on the wildland fire is direct, or parallel with the

A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome analysis.

PubMed

Lee, Hangyeore; Mun, Dong-Gi; Bae, Jingi; Kim, Hokeun; Oh, Se Yeon; Park, Young Soo; Lee, Jae-Hyuk; Lee, Sang-Won

2015-08-21

We report a new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system. The system employs only two nano-volume switching valves (a two-position four port valve and a two-position ten port valve) that direct solvent flows from two binary nano-pumps for parallel operation of two analytical columns and two solid phase extraction (SPE) columns. Despite the simple design, the sDO-UHPLC offers many advantageous features that include high duty cycle, back flushing sample injection for fast and narrow zone sample injection, online desalting, high separation resolution and high intra/inter-column reproducibility. This system was applied to analyze proteome samples not only in high throughput deep proteome profiling experiments but also in high throughput MRM experiments.

Reduction of Cross-Reactive Carbohydrate Determinants in Plant Foodstuff: Elucidation of Clinical Relevance and Implications for Allergy Diagnosis

PubMed Central

Brehler, Randolf; von Schaewen, Antje

2011-01-01

Background A longstanding debate in allergy is whether or not specific immunoglobulin-E antibodies (sIgE), recognizing cross-reactive carbohydrate determinants (CCD), are able to elicit clinical symptoms. In pollen and food allergy, ≥20% of patients display in-vitro CCD reactivity based on presence of α1,3-fucose and/or β1,2-xylose residues on N-glycans of plant (xylose/fucose) and insect (fucose) glycoproteins. Because the allergenicity of tomato glycoallergen Lyc e 2 was ascribed to N-glycan chains alone, this study aimed at evaluating clinical relevance of CCD-reduced foodstuff in patients with carbohydrate-specific IgE (CCD-sIgE). Methodology/Principal Findings Tomato and/or potato plants with stable reduction of Lyc e 2 (tomato) or CCD formation in general were obtained via RNA interference, and gene-silencing was confirmed by immunoblot analyses. Two different CCD-positive patient groups were compared: one with tomato and/or potato food allergy and another with hymenoptera-venom allergy (the latter to distinguish between CCD- and peptide-specific reactions in the food-allergic group). Non-allergic and CCD-negative food-allergic patients served as controls for immunoblot, basophil activation, and ImmunoCAP analyses. Basophil activation tests (BAT) revealed that Lyc e 2 is no key player among other tomato (glyco)allergens. CCD-positive patients showed decreased (re)activity with CCD-reduced foodstuff, most obvious in the hymenoptera venom-allergic but less in the food-allergic group, suggesting that in-vivo reactivity is primarily based on peptide- and not CCD-sIgE. Peptide epitopes remained unaffected in CCD-reduced plants, because CCD-negative patient sera showed reactivity similar to wild-type. In-house-made ImmunoCAPs, applied to investigate feasibility in routine diagnosis, confirmed BAT results at the sIgE level. Conclusions/Significance CCD-positive hymenoptera venom-allergic patients (control group) showed basophil activation despite no allergic symptoms towards tomato and potato. Therefore, this proof-of-principle study demonstrates feasibility of CCD-reduced foodstuff to minimize ‘false-positive results’ in routine serum tests. Despite confirming low clinical relevance of CCD antibodies, we identified one patient with ambiguous in-vitro results, indicating need for further component-resolved diagnosis. PMID:21423762

Timing generator of scientific grade CCD camera and its implementation based on FPGA technology

NASA Astrophysics Data System (ADS)

Si, Guoliang; Li, Yunfei; Guo, Yongfei

2010-10-01

The Timing Generator's functions of Scientific Grade CCD Camera is briefly presented: it generates various kinds of impulse sequence for the TDI-CCD, video processor and imaging data output, acting as the synchronous coordinator for time in the CCD imaging unit. The IL-E2TDI-CCD sensor produced by DALSA Co.Ltd. use in the Scientific Grade CCD Camera. Driving schedules of IL-E2 TDI-CCD sensor has been examined in detail, the timing generator has been designed for Scientific Grade CCD Camera. FPGA is chosen as the hardware design platform, schedule generator is described with VHDL. The designed generator has been successfully fulfilled function simulation with EDA software and fitted into XC2VP20-FF1152 (a kind of FPGA products made by XILINX). The experiments indicate that the new method improves the integrated level of the system. The Scientific Grade CCD camera system's high reliability, stability and low power supply are achieved. At the same time, the period of design and experiment is sharply shorted.

The Use of Aerosol Optical Depth in Estimating Trace Gas Emissions from Biomass Burning Plumes

NASA Astrophysics Data System (ADS)

Jones, N.; Paton-Walsh, C.; Wilson, S.; Meier, A.; Deutscher, N.; Griffith, D.; Murcray, F.

2003-12-01

We have observed significant correlations between aerosol optical depth (AOD) at 500 nm and column amounts of a number of biomass burning indicators (carbon monoxide, hydrogen cyanide, formaldehyde and ammonia) in bushfire smoke plumes over SE Australia during the 2001/2002 and 2002/2003 fire seasons from remote sensing measurements. The Department of Chemistry, University of Wollongong, operates a high resolution Fourier Transform Spectrometer (FTS), in the city of Wollongong, approximately 80 km south of Sydney. During the recent bushfires we collected over 1500 solar FTIR spectra directly through the smoke over Wollongong. The total column amounts of the biomass burning indicators were calculated using the profile retrieval software package SFIT2. Using the same solar beam, a small grating spectrometer equipped with a 2048 pixel CCD detector array, was used to calculate simultaneous aerosol optical depths. This dataset is therefore unique in its temporal sampling, location to active fires, and range of simultaneously measured constituents. There are several important applications of the AOD to gas column correlation. The estimation of global emissions from biomass burning currently has very large associated uncertainties. The use of visible radiances measured by satellites, and hence AOD, could significantly reduce these uncertainties by giving a direct estimate of global emissions of gases from biomass burning through application of the AOD to gas correlation. On a more local level, satellite-derived aerosol optical depth maps could be inverted to infer approximate concentration levels of smoke-related pollutants at the ground and in the lower troposphere, and thus can be used to determine the nature of any significant health impacts.

Characterizing the plasma of the Rotating Wall Machine

NASA Astrophysics Data System (ADS)

Hannum, David A.

The Rotating Wall Machine (RoWM) is a line-tied linear screw pinch built to study current-driven external kink modes. The plasma column is formed by an array of seven electrostatic washer guns which can also be biased to drive plasma current. The array allows independent control over the electron density ne and current density Jz profiles of the column. Internal measurements of the plasma have been made with singletip Langmuir and magnetic induction ("B-dot") probes for a range of bias currents (Ib = 0, 300, 500 A/gun). Streams from the individual guns are seen to merge at a distance of z ≈ 36 cm from the guns; the exact distance depends on the value of Ib. The density of the column is directly proportional to the Ohmic dissipation power, but the temperature stays at a low, uniform value (Te ≈ 3.5 eV) for each bias level. Electron densities are on the order of ne ˜10 20 m-3. The electron density expands radially (across the Bz guide field) as the plasma moves along the column, though the current density Jz mainly stays parallel to the field lines. The singletip Langmuir probe diagnostic is difficult to analyze for Ib = 500 A/gun plasmas and fails as Ib is raised beyond this level. Spectrographic analysis of the Halpha line indicates that the hydrogen plasmas are nearly fully ionized at each bias level. Azimuthal E x B rotation is axially and radially sheared; rotation slows as the plasma reaches the anode. Perpendicular diffusivity is consistent with the classical value, D⊥ ≈ 5 m2/sec, while parallel resistivity is seen to be twice the classical Spitzer value, 2 x 10-4 O m.

Fmoc-RGDS based fibrils: atomistic details of their hierarchical assembly.

PubMed

Zanuy, David; Poater, Jordi; Solà, Miquel; Hamley, Ian W; Alemán, Carlos

2016-01-14

We describe the 3D supramolecular structure of Fmoc-RGDS fibrils, where Fmoc and RGDS refer to the hydrophobic N-(fluorenyl-9-methoxycarbonyl) group and the hydrophilic Arg-Gly-Asp-Ser peptide sequence, respectively. For this purpose, we performed atomistic all-atom molecular dynamics simulations of a wide variety of packing modes derived from both parallel and antiparallel β-sheet configurations. The proposed model, which closely resembles the cross-β core structure of amyloids, is stabilized by π-π stacking interactions between hydrophobic Fmoc groups. More specifically, in this organization, the Fmoc-groups of β-strands belonging to the same β-sheet form columns of π-stacked aromatic rings arranged in a parallel fashion. Eight of such columns pack laterally forming a compact and dense hydrophobic core, in which two central columns are surrounded by three adjacent columns on each side. In addition to such Fmoc···Fmoc interactions, the hierarchical assembly of the constituent β-strands involves a rich variety of intra- and inter-strand interactions. Accordingly, hydrogen bonding, salt bridges and π-π stacking interactions coexist in the highly ordered packing network proposed for the Fmoc-RGDS amphiphile. Quantum mechanical calculations, which have been performed to quantify the above referred interactions, confirm the decisive role played by the π-π stacking interactions between the rings of the Fmoc groups, even though both inter-strand and intra-strand hydrogen bonds and salt bridges also play a non-negligible role. Overall, these results provide a solid reference to complement the available experimental data, which are not precise enough to determine the fibril structure, and reconcile previous independent observations.

Tensor contraction engine: Abstraction and automated parallel implementation of configuration-interaction, coupled-cluster, and many-body perturbation theories

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hirata, So

2003-11-20

We develop a symbolic manipulation program and program generator (Tensor Contraction Engine or TCE) that automatically derives the working equations of a well-defined model of second-quantized many-electron theories and synthesizes efficient parallel computer programs on the basis of these equations. Provided an ansatz of a many-electron theory model, TCE performs valid contractions of creation and annihilation operators according to Wick's theorem, consolidates identical terms, and reduces the expressions into the form of multiple tensor contractions acted by permutation operators. Subsequently, it determines the binary contraction order for each multiple tensor contraction with the minimal operation and memory cost, factorizes commonmore » binary contractions (defines intermediate tensors), and identifies reusable intermediates. The resulting ordered list of binary tensor contractions, additions, and index permutations is translated into an optimized program that is combined with the NWChem and UTChem computational chemistry software packages. The programs synthesized by TCE take advantage of spin symmetry, Abelian point-group symmetry, and index permutation symmetry at every stage of calculations to minimize the number of arithmetic operations and storage requirement, adjust the peak local memory usage by index range tiling, and support parallel I/O interfaces and dynamic load balancing for parallel executions. We demonstrate the utility of TCE through automatic derivation and implementation of parallel programs for various models of configuration-interaction theory (CISD, CISDT, CISDTQ), many-body perturbation theory [MBPT(2), MBPT(3), MBPT(4)], and coupled-cluster theory (LCCD, CCD, LCCSD, CCSD, QCISD, CCSDT, and CCSDTQ).« less

Minimal Power Latch for Single-Slope ADCs

NASA Technical Reports Server (NTRS)

Hancock, Bruce R.

2013-01-01

Column-parallel analog-to-digital converters (ADCs) for imagers involve simultaneous operation of many ADCs. Single-slope ADCs are well adapted to this use because of their simplicity. Each ADC contains a comparator, comparing its input signal level to an increasing reference signal (ramp). When the ramp is equal to the input, the comparator triggers a latch that captures an encoded counter value (code). Knowing the captured code, the ramp value and hence the input signal are determined. In a column-parallel ADC, each column contains only the comparator and the latches; the ramp and code generation are shared. In conventional latch or flip-flop circuits, there is an input stage that tracks the input signal, and this stage consumes switching current every time the input changes. With many columns, many bits, and high code rates, this switching current can be substantial. It will also generate noise that may corrupt the analog signals. A latch was designed that does not track the input, and consumes power only at the instant of latching the data value. The circuit consists of two S-R (set-reset) latches, gated by the comparator. One is set by high data values and the other by low data values. The latches are cross-coupled so that the first one to set blocks the other. In order that the input data not need an inversion, which would consume power, the two latches are made in complementary polarity. This requires complementary gates from the comparator, instead of complementary data values, but the comparator only triggers once per conversion, and usually has complementary outputs to begin with. An efficient CMOS (complementary metal oxide semiconductor) implementation of this circuit is shown in the figure, where C is the comparator output, D is the data (code), and Q0 and Q1 are the outputs indicating the capture of a zero or one value. The latch for Q0 has a negative-true set signal and output, and is implemented using OR-AND-INVERT logic, while the latch for Q1 uses positive- true signals and is implemented using AND-OR-INVERT logic. In this implementation, both latches are cleared when the comparator is reset. Two redundant transistors are removed from the reset side of each latch, making for a compact layout. CMOS imagers with column-parallel ADCs have demonstrated high performance for remote sensing applications. With this latch circuit, the power consumption and noise can be further reduced. This innovation can be used in CMOS imagers and very-low-power electronics

CCD Photometer Installed on the Telescope - 600 OF the Shamakhy Astrophysical Observatory II. The Technique of Observation and Data Processing of CCD Photometry

NASA Astrophysics Data System (ADS)

Abdullayev, B. I.; Gulmaliyev, N. I.; Majidova, S. O.; Mikayilov, Kh. M.; Rustamov, B. N.

2009-12-01

Basic technical characteristics of CCD matrix U-47 made by the Apogee Alta Instruments Inc. are provided. Short description and features of various noises introduced by optical system and CCD camera are presented. The technique of getting calibration frames: bias, dark, flat field and main stages of processing of results CCD photometry are described.

Myria: Scalable Analytics as a Service

NASA Astrophysics Data System (ADS)

Howe, B.; Halperin, D.; Whitaker, A.

2014-12-01

At the UW eScience Institute, we're working to empower non-experts, especially in the sciences, to write and use data-parallel algorithms. To this end, we are building Myria, a web-based platform for scalable analytics and data-parallel programming. Myria's internal model of computation is the relational algebra extended with iteration, such that every program is inherently data-parallel, just as every query in a database is inherently data-parallel. But unlike databases, iteration is a first class concept, allowing us to express machine learning tasks, graph traversal tasks, and more. Programs can be expressed in a number of languages and can be executed on a number of execution environments, but we emphasize a particular language called MyriaL that supports both imperative and declarative styles and a particular execution engine called MyriaX that uses an in-memory column-oriented representation and asynchronous iteration. We deliver Myria over the web as a service, providing an editor, performance analysis tools, and catalog browsing features in a single environment. We find that this web-based "delivery vector" is critical in reaching non-experts: they are insulated from irrelevant effort technical work associated with installation, configuration, and resource management. The MyriaX backend, one of several execution runtimes we support, is a main-memory, column-oriented, RDBMS-on-the-worker system that supports cyclic data flows as a first-class citizen and has been shown to outperform competitive systems on 100-machine cluster sizes. I will describe the Myria system, give a demo, and present some new results in large-scale oceanographic microbiology.

Comparative analysis of data quality and applications in vegetation of HJ-1A CCD images

NASA Astrophysics Data System (ADS)

Wei, Hongwei; Tian, Qingjiu; Huang, Yan; Wang, Yan

2014-05-01

To study the data quality and to find the differences in vegetation monitoring applications, the same region at Chuzhou Lai 'an, the data of HJ-1A CCD1 on the April 1st, 2012 and the data of HJ-1A CCD2 on the March 31, 2012 have being comparative analysis by the method of objective quality (image)assessment which selecting over five spectral image evaluation parameters: radiation precision (mean, variance, inclination, steepness), information entropy, signal-to-noise ratio, sharpness, contrast, and normalized differential vegetation index. The results show that there is little differences between the HJ-1A CCD1 and CCD2 by objective evaluation of data quality except radiation precision conform to their design theory, so the conclusion is that the difference of them without considering on the usual unless continuation;and Combination of field observation data Lai'an spectral data and GPS data (each point),selecting the normalized difference vegetation index as CCD1, CCD2 in vegetation monitoring application on the evaluation of the differences, and the specific process is based on GPS data is divided into nine small plots of spectral data ,and image data of nine one-to-one correspondence plots, and their normalized difference vegetation index values were calculated ,and measured spectra data resampling HJ-1A CCD1, CCD2 spectral response function calculated NDVI, and the results show that there is little differences between the HJ-1A CCD1 and CCD2 by objective evaluation of data quality, and, the differences of wheat `s reflection and normalized vegetation index is mainly due to calibration coefficients of CCD1 and CCD2, the differences of the solar elevation angle when obtaining the image and atmospheric conditions, so it has to consider the performance indicators as well as access conditions of CCD1 and CCD2, and to be take the normalization techniques for processing for the comparison analysis in the use of HJ-1A CCD Data to surface dynamic changes; Finally, in order to study the response of the spectral response function proposed spectral response function of impact factor, and in view of the spectral response function measured spectral data resampling only HJ-1A CCD spectral response function, calculated according to the formula of the equivalent reflectivity quantitative spectral response function, and spectral normalization of proposed theoretical Technical Support. The Objective evaluation of its application of HJ-1A CCD1, and CCD2 data quality differences research has important implications for broader application to further promote China-made remote sensing satellite data, future research also needs calibration coefficient, the solar elevation angle atmospheric conditions and its image scanning angle be taken into account, and to make the corresponding normalized its impact quantitative research has important significance for the timing changes in the application of the ecological environment in China.

Philtral columns and nostril shapes in nigerian children: a morphometric and aesthetic analysis.

PubMed

Abdulrasheed, Ibrahim; Eneye, Asuku Malachy

2013-01-01

Background. The upper lip-nose complex contributes significantly to the concept of symmetry and proportion of the face. A study of the morphology and aesthetic preferences of the lip-nose complex will provide a database that will serve as a guide for reconstruction. Subjects and Methods. Hundred Nigerian children participated in this study. Demographic data and standard photographs of the philtral column and nostrils were obtained. Sixty volunteers were recruited to evaluate the photographs. Each volunteer was asked to rank the photographs based on their aesthetic preference. Results. The morphology of the philtral columns was classified into four groups: (1) triangular, (2) concave, (3) flat, and (4) parallel. The nostril shape was also classified into four groups: (1) triangular, (2) round, (3) teardrop, and (4) rectangular. In both genders, the triangular shape of philtral column was the most common. There are significant age differences in the aesthetic rankings of philtral columns and nostril shapes. Conclusion. Our study establishes the basal values for the morphometric and aesthetic parameters of the lip-nose complex of 5- and 6-year-old children in Nigeria. We hope our results and reconstructive surgery will intersect at a point to treat disfigurements of the philtrum and nostrils successfully.

Technical guidance for the development of a solid state image sensor for human low vision image warping

NASA Technical Reports Server (NTRS)

Vanderspiegel, Jan

1994-01-01

This report surveys different technologies and approaches to realize sensors for image warping. The goal is to study the feasibility, technical aspects, and limitations of making an electronic camera with special geometries which implements certain transformations for image warping. This work was inspired by the research done by Dr. Juday at NASA Johnson Space Center on image warping. The study has looked into different solid-state technologies to fabricate image sensors. It is found that among the available technologies, CMOS is preferred over CCD technology. CMOS provides more flexibility to design different functions into the sensor, is more widely available, and is a lower cost solution. By using an architecture with row and column decoders one has the added flexibility of addressing the pixels at random, or read out only part of the image.

Differential high-speed digital micromirror device based fluorescence speckle confocal microscopy.

PubMed

Jiang, Shihong; Walker, John

2010-01-20

We report a differential fluorescence speckle confocal microscope that acquires an image in a fraction of a second by exploiting the very high frame rate of modern digital micromirror devices (DMDs). The DMD projects a sequence of predefined binary speckle patterns to the sample and modulates the intensity of the returning fluorescent light simultaneously. The fluorescent light reflecting from the DMD's "on" and "off" pixels is modulated by correlated speckle and anticorrelated speckle, respectively, to form two images on two CCD cameras in parallel. The sum of the two images recovers a widefield image, but their difference gives a near-confocal image in real time. Experimental results for both low and high numerical apertures are shown.

Recent Changes in Tropospheric Ozone in the Tropics

NASA Technical Reports Server (NTRS)

Chandra, S.; Ziemke, J. R.; Einaudi, Franco (Technical Monitor)

2000-01-01

This paper presents a detailed characterization of tropical tropospheric column ozone variability on time scales varying from a few days to a solar cycle. The study is based on more than 20 years (1979 to the present) of tropospheric column ozone time series derived from the convective cloud differential (CCD) method using total ozone mapping spectrometer (TOMS) data. Results indicate three distinct regions in the tropics with distinctly three different zonal characteristics related to seasonal, interannual and solar variabilities. These three regions are the eastern Pacific, Atlantic, and western Pacific. Tropospheric column ozone in the Atlantic region peaks at about the same time (September-October) from 20 N to 20 S. The amplitude of the annual cycle, however, varies from about 3 to 6 Dobson unit (DU) from north to south of the equator. In comparison, the annual cycle in both the eastern and western Pacific is generally week and the phase varies from peak values in March and April in the northern hemisphere to September and October in the southern hemisphere. The interannual pattern in the three regions are also very different. The Atlantic region indicates a quasi biennial oscillation in the tropospheric column ozone which is out of phase with the stratospheric ozone. This is consistent with the photochemical control of this region caused by high pollution and high concentration of ozone producing precursors. The observed pattern, however, does not seem to be related to the interannual variability in ozone precursors related to biomass burning. Instead, it appears to be a manifestation of the UV modulation of upper tropospheric chemistry on a QBO time scale caused by stratospheric ozone. During El Nino events, there is anomalously low ozone in the eastern Pacific and high values in the western Pacific indicating the effects of convectively driven transport. The observed increase of 10-20 DU in tropospheric column ozone in the Indonesian region in the western Pacific during the recent 1997-1998 El Nino was associated with large-scale forest fires which may have contributed 5-10 DU of the total increase.

Multi-Column Experimental Test Bed Using CaSDB MOF for Xe/Kr Separation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Welty, Amy Keil; Greenhalgh, Mitchell Randy; Garn, Troy Gerry

Processing of spent nuclear fuel produces off-gas from which several volatile radioactive components must be separated for further treatment or storage. As part of the Off-gas Sigma Team, parallel research at INL and PNNL has produced several promising sorbents for the selective capture of xenon and krypton from these off-gas streams. In order to design full-scale treatment systems, sorbents that are promising on a laboratory scale must be proven under process conditions to be considered for pilot and then full-scale use. To that end, a bench-scale multi-column system with capability to test multiple sorbents was designed and constructed at INL.more » This report details bench-scale testing of CaSDB MOF, produced at PNNL, and compares the results to those reported last year using INL engineered sorbents. Two multi-column tests were performed with the CaSDB MOF installed in the first column, followed with HZ-PAN installed in the second column. The CaSDB MOF column was placed in a Stirling cryocooler while the cryostat was employed for the HZ-PAN column. Test temperatures of 253 K and 191 K were selected for the first column while the second column was held at 191 K for both tests. Calibrated volume sample bombs were utilized for gas stream analyses. At the conclusion of each test, samples were collected from each column and analyzed for gas composition. While CaSDB MOF does appear to have good capacity for Xe, the short time to initial breakthrough would make design of a continuous adsorption/desorption cycle difficult, requiring either very large columns or a large number of smaller columns. Because of the tenacity with which Xe and Kr adhere to the material once adsorbed, this CaSDB MOF may be more suitable for use as a long-term storage solution. Further testing is recommended to determine if CaSDB MOF is suitable for this purpose.« less

CCD TV focal plane guider development and comparison to SIRTF applications

NASA Technical Reports Server (NTRS)

Rank, David M.

1989-01-01

It is expected that the SIRTF payload will use a CCD TV focal plane fine guidance sensor to provide acquisition of sources and tracking stability of the telescope. Work has been done to develop CCD TV cameras and guiders at Lick Observatory for several years and have produced state of the art CCD TV systems for internal use. NASA decided to provide additional support so that the limits of this technology could be established and a comparison between SIRTF requirements and practical systems could be put on a more quantitative basis. The results of work carried out at Lick Observatory which was designed to characterize present CCD autoguiding technology and relate it to SIRTF applications is presented. Two different design types of CCD cameras were constructed using virtual phase and burred channel CCD sensors. A simple autoguider was built and used on the KAO, Mt. Lemon and Mt. Hamilton telescopes. A video image processing system was also constructed in order to characterize the performance of the auto guider and CCD cameras.

A metagenomic survey of microbes in honey bee colony collapse disorder.

PubMed

Cox-Foster, Diana L; Conlan, Sean; Holmes, Edward C; Palacios, Gustavo; Evans, Jay D; Moran, Nancy A; Quan, Phenix-Lan; Briese, Thomas; Hornig, Mady; Geiser, David M; Martinson, Vince; vanEngelsdorp, Dennis; Kalkstein, Abby L; Drysdale, Andrew; Hui, Jeffrey; Zhai, Junhui; Cui, Liwang; Hutchison, Stephen K; Simons, Jan Fredrik; Egholm, Michael; Pettis, Jeffery S; Lipkin, W Ian

2007-10-12

In colony collapse disorder (CCD), honey bee colonies inexplicably lose their workers. CCD has resulted in a loss of 50 to 90% of colonies in beekeeping operations across the United States. The observation that irradiated combs from affected colonies can be repopulated with naive bees suggests that infection may contribute to CCD. We used an unbiased metagenomic approach to survey microflora in CCD hives, normal hives, and imported royal jelly. Candidate pathogens were screened for significance of association with CCD by the examination of samples collected from several sites over a period of 3 years. One organism, Israeli acute paralysis virus of bees, was strongly correlated with CCD.

Silicide Schottky Barrier For Back-Surface-Illuminated CCD

NASA Technical Reports Server (NTRS)

Hecht, Michael H.

1990-01-01

Quantum efficiency of back-surface-illuminated charge-coupled device (CCD) increased by coating back surface with thin layer of PtSi or IrSi on thin layer of SiO2. In its interaction with positively-doped bulk Si of CCD, silicide/oxide layer forms Schottky barrier that repels electrons, promoting accumulation of photogenerated charge carriers in front-side CCD potential wells. Physical principle responsible for improvement explained in "Metal Film Increases CCD Output" (NPO-16815).

Arts and Crafts for Use with Mentally Retarded Students.

ERIC Educational Resources Information Center

Child, Toni, Ed.

GRADES OR AGES: Mentally retarded children of any age. SUBJECT MATTER: Arts and crafts. ORGANIZATION AND PHYSICAL APPEARANCE: The guide is divided into sections according to the material to be used. Section headings are: Paper, Fabrics, Wood, Metal, Nature, Miscellaneous, and Painting. Each section is laid out in three parallel columns, one each…

Continuous-time ΣΔ ADC with implicit variable gain amplifier for CMOS image sensor.

PubMed

Tang, Fang; Bermak, Amine; Abbes, Amira; Benammar, Mohieddine Amor

2014-01-01

This paper presents a column-parallel continuous-time sigma delta (CTSD) ADC for mega-pixel resolution CMOS image sensor (CIS). The sigma delta modulator is implemented with a 2nd order resistor/capacitor-based loop filter. The first integrator uses a conventional operational transconductance amplifier (OTA), for the concern of a high power noise rejection. The second integrator is realized with a single-ended inverter-based amplifier, instead of a standard OTA. As a result, the power consumption is reduced, without sacrificing the noise performance. Moreover, the variable gain amplifier in the traditional column-parallel read-out circuit is merged into the front-end of the CTSD modulator. By programming the input resistance, the amplitude range of the input current can be tuned with 8 scales, which is equivalent to a traditional 2-bit preamplification function without consuming extra power and chip area. The test chip prototype is fabricated using 0.18 μm CMOS process and the measurement result shows an ADC power consumption lower than 63.5 μW under 1.4 V power supply and 50 MHz clock frequency.

Performance characteristics of CCDs for the ACIS experiment. [Advanced X-ray Astrophysics Facility CCD Imaging Spectrometer

NASA Technical Reports Server (NTRS)

Garmire, Gordon P.; Nousek, John; Burrows, David; Ricker, George; Bautz, Mark; Doty, John; Collins, Stewart; Janesick, James

1988-01-01

The search for the optimum CCD to be used at the focal surface of the Advanced X-ray Astrophysics Facility (AXAF) is described. The physics of the interaction of X-rays in silicon through the photoelectric effect is reviewed. CCD technology at the beginning of the AXAF definition phase is summarized, and the results of the CCD enhancement program are discussed. Other sources of optimum CCDs are examined, and CCD enhancements made at MIT Lincoln Laboratory are addressed.

CCD research. [design, fabrication, and applications

NASA Technical Reports Server (NTRS)

Gassaway, J. D.

1976-01-01

The fundamental problems encountered in designing, fabricating, and applying CCD's are reviewed. Investigations are described and results and conclusions are given for the following: (1) the development of design analyses employing computer aided techniques and their application to the design of a grapped structure; (2) the role of CCD's in applications to electronic functions, in particular, signal processing; (3) extending the CCD to silicon films on sapphire (SOS); and (4) all aluminum transfer structure with low noise input-output circuits. Related work on CCD imaging devices is summarized.

Methods for controlling pore morphology in aerogels using electric fields and products thereof

DOE Office of Scientific and Technical Information (OSTI.GOV)

Worsley, Marcus A.; Baumann, Theodore F.; Satcher, Jr., Joe H.

In one embodiment, an aerogel or xerogel includes column structures of a material having minor pores therein and major pores devoid of the material positioned between the column structures, where longitudinal axes of the major pores are substantially parallel to one another. In another embodiment, a method includes heating a sol including aerogel or xerogel precursor materials to cause gelation thereof to form an aerogel or xerogel and exposing the heated sol to an electric field, wherein the electric field causes orientation of a microstructure of the sol during gelation, which is retained by the aerogel or xerogel. In onemore » approach, an aerogel has elongated pores extending between a material arranged in column structures having structural characteristics of being formed from a sol exposed to an electric field that causes orientation of a microstructure of the sol during gelation which is retained by the elongated pores of the aerogel.« less

A GC-system for the analysis of residual geothermal gases

USGS Publications Warehouse

Sheppard, D.S.; Truesdell, A.H.

1985-01-01

The gases evolved from geothermal fields, after condensation of H2O, CO2, H2S and NH3 in caustic solution, contain He, H2, Ar, O2, N2, CH4 and higher hydrocarbons. The analysis for the major components in these residual gas mixtures can be achieved by use of two simple gas chromatographs in parallel, and using 5A?? molecular sieve. The separation of He and H2 to baseline is achieved by using low temperatures (30??C) coupled with a relatively long column; and the difficult separation of Ar and O2 is achieved by use of a cryogenically cooled column. The use of switching valves to backflush and bypass columns ensures that a minimum time for analysis can be achieved whilst retaining baseline separations of the He/H2 and Ar/O2 pairs. ?? 1985 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

Total ionizing dose effect and damage mechanism on saturation output voltage of charge coupled device

NASA Astrophysics Data System (ADS)

Wen, Lin; Li, Yu-dong; Guo, Qi; Wang, Chao-min

2018-02-01

Total ionizing dose effect is a major threat to space applications of CCD, which leads to the decrease of CCD saturation output voltage and the increase of dark signal. This paper investigated CCD and its readout circuit for experimental samples of different channel width to length ratio of MOSFET, and readout circuit amplifier, and CCD. The irradiation source was 60Co- gamma ray. through testing the parameters degradation of MOSFET and amplifier degradation, the generation and annealing law of irradiation induced defects in MOS single tube are analyzed. Combined with the radiation effect of amplifier and CCD, The correlation of radiation damage of the MOSFET and the readout circuit amplifier and CCD parameter degradation is established. Finally, this paper reveals the physical mechanism of ionizing radiation damage of the readout circuit. The research results provide a scientific basis for the selection of anti-radiation technology and structure optimization of domestic CCD.

Experimental study of the stability and flow characteristics of floating liquid columns confined between rotating disks

NASA Technical Reports Server (NTRS)

Fowle, A. A.; Soto, L.; Strong, P. F.; Wang, C. A.

1980-01-01

A low Bond number simulation technique was used to establish the stability limits of cylindrical and conical floating liquid columns under conditions of isorotation, equal counter rotation, rotation of one end only, and parallel axis offset. The conditions for resonance in cylindrical liquid columns perturbed by axial, sinusoidal vibration of one end face are also reported. All tests were carried out under isothermal conditions with water and silicone fluids of various viscosities. A technique for the quantitative measurement of stream velocity within a floating, isothermal, liquid column confined between rotatable disks was developed. In the measurement, small, light scattering particles were used as streamline markers in common arrangement, but the capability of the measurement was extended by use of stereopair photography system to provide quantitative data. Results of velocity measurements made under a few selected conditions, which established the precision and accuracy of the technique, are given. The general qualitative features of the isothermal flow patterns under various conditions of end face rotation resulting from both still photography and motion pictures are presented.

Analysis of programming properties and the row-column generation method for 1-norm support vector machines.

PubMed

Zhang, Li; Zhou, WeiDa

2013-12-01

This paper deals with fast methods for training a 1-norm support vector machine (SVM). First, we define a specific class of linear programming with many sparse constraints, i.e., row-column sparse constraint linear programming (RCSC-LP). In nature, the 1-norm SVM is a sort of RCSC-LP. In order to construct subproblems for RCSC-LP and solve them, a family of row-column generation (RCG) methods is introduced. RCG methods belong to a category of decomposition techniques, and perform row and column generations in a parallel fashion. Specially, for the 1-norm SVM, the maximum size of subproblems of RCG is identical with the number of Support Vectors (SVs). We also introduce a semi-deleting rule for RCG methods and prove the convergence of RCG methods when using the semi-deleting rule. Experimental results on toy data and real-world datasets illustrate that it is efficient to use RCG to train the 1-norm SVM, especially in the case of small SVs. Copyright © 2013 Elsevier Ltd. All rights reserved.

Parallel computation of level set method for 500 Hz visual servo control

NASA Astrophysics Data System (ADS)

Fei, Xianfeng; Igarashi, Yasunobu; Hashimoto, Koichi

2008-11-01

We propose a 2D microorganism tracking system using a parallel level set method and a column parallel vision system (CPV). This system keeps a single microorganism in the middle of the visual field under a microscope by visual servoing an automated stage. We propose a new energy function for the level set method. This function constrains an amount of light intensity inside the detected object contour to control the number of the detected objects. This algorithm is implemented in CPV system and computational time for each frame is 2 [ms], approximately. A tracking experiment for about 25 s is demonstrated. Also we demonstrate a single paramecium can be kept tracking even if other paramecia appear in the visual field and contact with the tracked paramecium.

Method and structure for skewed block-cyclic distribution of lower-dimensional data arrays in higher-dimensional processor grids

DOEpatents

Chatterjee, Siddhartha [Yorktown Heights, NY; Gunnels, John A [Brewster, NY

2011-11-08

A method and structure of distributing elements of an array of data in a computer memory to a specific processor of a multi-dimensional mesh of parallel processors includes designating a distribution of elements of at least a portion of the array to be executed by specific processors in the multi-dimensional mesh of parallel processors. The pattern of the designating includes a cyclical repetitive pattern of the parallel processor mesh, as modified to have a skew in at least one dimension so that both a row of data in the array and a column of data in the array map to respective contiguous groupings of the processors such that a dimension of the contiguous groupings is greater than one.

Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting

PubMed Central

2014-01-01

Background In saffron (Crocus sativus), new corms develop at the base of every shoot developed from the maternal corm, a globular underground storage stem. Since the degree of bud sprouts influences the number and size of new corms, and strigolactones (SLs) suppress growth of pre-formed axillary bud, it was considered appropriate to investigate SL involvement in physiology and molecular biology in saffron. We focused on two of the genes within the SL pathway, CCD7 and CCD8, encoding carotenoid cleavage enzymes required for the production of SLs. Results The CsCCD7 and CsCCD8 genes are the first ones isolated and characterized from a non-grass monocotyledonous plant. CsCCD7 and CsCCD8 expression showed some overlapping, although they were not identical. CsCCD8 was highly expressed in quiescent axillary buds and decapitation dramatically reduced its expression levels, suggesting its involvement in the suppression of axillary bud outgrowth. Furthermore, in vitro experiments showed also the involvement of auxin, cytokinin and jasmonic acid on the sprouting of axillary buds from corms in which the apical bud was removed. In addition, CsCCD8 expression, but not CsCCD7, was higher in the newly developed vascular tissue of axillary buds compared to the vascular tissue of the apical bud. Conclusions We showed that production and transport of auxin in saffron corms could act synergistically with SLs to arrest the outgrowth of the axillary buds, similar to the control of above-ground shoot branching. In addition, jasmonic acid seems to play a prominent role in bud dormancy in saffron. While cytokinins from roots promote bud outgrowth. In addition the expression results of CsCCD8 suggest that SLs could positively regulate procambial activity and the development of new vascular tissues connecting leaves with the mother corm. PMID:24947472

A parallelizable real-time motion tracking algorithm with applications to ultrasonic strain imaging.

PubMed

Jiang, J; Hall, T J

2007-07-07

Ultrasound-based mechanical strain imaging systems utilize signals from conventional diagnostic ultrasound systems to image tissue elasticity contrast that provides new diagnostically valuable information. Previous works (Hall et al 2003 Ultrasound Med. Biol. 29 427, Zhu and Hall 2002 Ultrason. Imaging 24 161) demonstrated that uniaxial deformation with minimal elevation motion is preferred for breast strain imaging and real-time strain image feedback to operators is important to accomplish this goal. The work reported here enhances the real-time speckle tracking algorithm with two significant modifications. One fundamental change is that the proposed algorithm is a column-based algorithm (a column is defined by a line of data parallel to the ultrasound beam direction, i.e. an A-line), as opposed to a row-based algorithm (a row is defined by a line of data perpendicular to the ultrasound beam direction). Then, displacement estimates from its adjacent columns provide good guidance for motion tracking in a significantly reduced search region to reduce computational cost. Consequently, the process of displacement estimation can be naturally split into at least two separated tasks, computed in parallel, propagating outward from the center of the region of interest (ROI). The proposed algorithm has been implemented and optimized in a Windows system as a stand-alone ANSI C++ program. Results of preliminary tests, using numerical and tissue-mimicking phantoms, and in vivo tissue data, suggest that high contrast strain images can be consistently obtained with frame rates (10 frames s(-1)) that exceed our previous methods.

Impact of phenanthrene on the properties of biogeochemical interfaces in soil: A two-layer column study

NASA Astrophysics Data System (ADS)

Reichel, Katharina; Totsche, Kai Uwe

2013-04-01

Biogeochemical interfaces in soils (Totsche et al. 2010) are the "hot spots" of microbial activity and the processing of organic compounds in soils. The production and relocation of mobile organic matter (MOM) and biocolloids like microorganisms are key processes for the formation and depth propagation of biogeochemical interfaces in soils (BGI). Phenanthrene (PHE) has been shown to affect microbial communities in soils (Ding et al. 2012) and may induce shifts in MOM quantity and quality (amount, type and properties of MOM). We hypothesize that the properties of BGI in soil change significantly due to the presence of PHE. The objectives of this study are (i) to evaluate the effect of PHE on soil microbial communities and on MOM quantity and quality under flow conditions with single- and two-layer column experiments and (ii) to assess the role of these processes for the physicochemical, mechanical and sorptive properties of BGI in soils. The soil columns were operated under water-unsaturated conditions. The top layer (source layer, SL, 2 cm) is made of sieved soil material (Luvisol, Scheyern, Germany) spiked with PHE (0.2 mg/g). The bottom layer (reception layer, RL, 10 cm) comprised the same soil without PHE. PHE-free columns were conducted in parallel as reference. Release and transport of MOM in mature soil of a single-layer column experiment was found to depend on the transport regime. The release of larger sized MOM (>0.45 µm) was restricted to an increased residence time during flow interruptions. Steady flow conditions favor the release of smaller MOM (<0.45 µm). Compared to the reference, in the two-layer column experiments higher OC concentrations were detected in the effluent from PHE spiked columns after enhanced flow interruptions (26d, 52d). That indicated the PHE influenced production or mobilization of MOM. Parallel factor analysis of fluorescence excitation and emission matrices revealed the presence of a constant DOM background and two new unknown components in the effluent, probably PHE metabolites. The emergence of new components emphasizes the role of metabolization processes in the release of MOM. The identification of key microbial actors and communities are currently in progress. Totsche, K.U. et al. (2010): Biogeochemical interfaces in soil: The interdisciplinary challenge for soil science. J. Plant Nutr. Soil Sci., 173(1), 88-99 Ding, G.-C., Heuer, H. & Smalla, K. (2012): Dynamics of bacterial communities in two unpolluted soils after spiking with phenanthrene: soil type specific and common responders. Front Microbio 10.3389/fmicb.2012.00290.

Columnar Segregation of Magnocellular and Parvocellular Streams in Human Extrastriate Cortex

PubMed Central

2017-01-01

Magnocellular versus parvocellular (M-P) streams are fundamental to the organization of macaque visual cortex. Segregated, paired M-P streams extend from retina through LGN into V1. The M stream extends further into area V5/MT, and parts of V2. However, elsewhere in visual cortex, it remains unclear whether M-P-derived information (1) becomes intermixed or (2) remains segregated in M-P-dominated columns and neurons. Here we tested whether M-P streams exist in extrastriate cortical columns, in 8 human subjects (4 female). We acquired high-resolution fMRI at high field (7T), testing for M- and P-influenced columns within each of four cortical areas (V2, V3, V3A, and V4), based on known functional distinctions in M-P streams in macaque: (1) color versus luminance, (2) binocular disparity, (3) luminance contrast sensitivity, (4) peak spatial frequency, and (5) color/spatial interactions. Additional measurements of resting state activity (eyes closed) tested for segregated functional connections between these columns. We found M- and P-like functions and connections within and between segregated cortical columns in V2, V3, and (in most experiments) area V4. Area V3A was dominated by the M stream, without significant influence from the P stream. These results suggest that M-P streams exist, and extend through, specific columns in early/middle stages of human extrastriate cortex. SIGNIFICANCE STATEMENT The magnocellular and parvocellular (M-P) streams are fundamental components of primate visual cortical organization. These streams segregate both anatomical and functional properties in parallel, from retina through primary visual cortex. However, in most higher-order cortical sites, it is unknown whether such M-P streams exist and/or what form those streams would take. Moreover, it is unknown whether M-P streams exist in human cortex. Here, fMRI evidence measured at high field (7T) and high resolution revealed segregated M-P streams in four areas of human extrastriate cortex. These results suggest that M-P information is processed in segregated parallel channels throughout much of human visual cortex; the M-P streams are more than a convenient sorting property in earlier stages of the visual system. PMID:28724749

Computer vision for driver assistance systems

NASA Astrophysics Data System (ADS)

Handmann, Uwe; Kalinke, Thomas; Tzomakas, Christos; Werner, Martin; von Seelen, Werner

1998-07-01

Systems for automated image analysis are useful for a variety of tasks and their importance is still increasing due to technological advances and an increase of social acceptance. Especially in the field of driver assistance systems the progress in science has reached a level of high performance. Fully or partly autonomously guided vehicles, particularly for road-based traffic, pose high demands on the development of reliable algorithms due to the conditions imposed by natural environments. At the Institut fur Neuroinformatik, methods for analyzing driving relevant scenes by computer vision are developed in cooperation with several partners from the automobile industry. We introduce a system which extracts the important information from an image taken by a CCD camera installed at the rear view mirror in a car. The approach consists of a sequential and a parallel sensor and information processing. Three main tasks namely the initial segmentation (object detection), the object tracking and the object classification are realized by integration in the sequential branch and by fusion in the parallel branch. The main gain of this approach is given by the integrative coupling of different algorithms providing partly redundant information.

Characterization of OfWRKY3, a transcription factor that positively regulates the carotenoid cleavage dioxygenase gene OfCCD4 in Osmanthus fragrans.

PubMed

Han, Yuanji; Wu, Miao; Cao, Liya; Yuan, Wangjun; Dong, Meifang; Wang, Xiaohui; Chen, Weicai; Shang, Fude

2016-07-01

The sweet osmanthus carotenoid cleavage dioxygenase 4 (OfCCD4) cleaves carotenoids such as β-carotene and zeaxanthin to yield β-ionone. OfCCD4 is a member of the CCD gene family, and its promoter contains a W-box palindrome with two reversely oriented TGAC repeats, which are the proposed binding sites of WRKY transcription factors. We isolated three WRKY cDNAs from the petal of Osmanthus fragrans. One of them, OfWRKY3, encodes a protein containing two WRKY domains and two zinc finger motifs. OfWRKY3 and OfCCD4 had nearly identical expression profile in petals of 'Dangui' and 'Yingui' at different flowering stages and showed similar expression patterns in petals treated by salicylic acid, jasmonic acid and abscisic acid. Activation of OfCCD4pro:GUS by OfWRKY3 was detected in coinfiltrated tobacco leaves and very weak GUS activity was detected in control tissues, indicating that OfWRKY3 can interact with the OfCCD4 promoter. Yeast one-hybrid and electrophoretic mobility shift assay showed that OfWRKY3 was able to bind to the W-box palindrome motif present in the OfCCD4 promoter. These results suggest that OfWRKY3 is a positive regulator of the OfCCD4 gene, and might partly account for the biosynthesis of β-ionone in sweet osmanthus.

High-resolution CCD imaging alternatives

NASA Astrophysics Data System (ADS)

Brown, D. L.; Acker, D. E.

1992-08-01

High resolution CCD color cameras have recently stimulated the interest of a large number of potential end-users for a wide range of practical applications. Real-time High Definition Television (HDTV) systems are now being used or considered for use in applications ranging from entertainment program origination through digital image storage to medical and scientific research. HDTV generation of electronic images offers significant cost and time-saving advantages over the use of film in such applications. Further in still image systems electronic image capture is faster and more efficient than conventional image scanners. The CCD still camera can capture 3-dimensional objects into the computing environment directly without having to shoot a picture on film develop it and then scan the image into a computer. 2. EXTENDING CCD TECHNOLOGY BEYOND BROADCAST Most standard production CCD sensor chips are made for broadcast-compatible systems. One popular CCD and the basis for this discussion offers arrays of roughly 750 x 580 picture elements (pixels) or a total array of approximately 435 pixels (see Fig. 1). FOR. A has developed a technique to increase the number of available pixels for a given image compared to that produced by the standard CCD itself. Using an inter-lined CCD with an overall spatial structure several times larger than the photo-sensitive sensor areas each of the CCD sensors is shifted in two dimensions in order to fill in spatial gaps between adjacent sensors.

Spectral analysis using the CCD Chirp Z-transform

NASA Technical Reports Server (NTRS)

Eversole, W. L.; Mayer, D. J.; Bosshart, P. W.; Dewit, M.; Howes, C. R.; Buss, D. D.

1978-01-01

The charge coupled device (CCD) Chirp Z transformation (CZT) spectral analysis techniques were reviewed and results on state-of-the-art CCD CZT technology are presented. The CZT algorithm was examined and the advantages of CCD implementation are discussed. The sliding CZT which is useful in many spectral analysis applications is described, and the performance limitations of the CZT are studied.

Documentation to the 2015-16 Common Core of Data (CCD) Universe Files. NCES 2017-074

ERIC Educational Resources Information Center

Glander, Mark

2017-01-01

The Common Core of Data (CCD) is a national statistical program that collects and compiles administrative data from SEAs covering the universe of all public elementary and secondary schools and school districts in the United States. The first CCD collection was for SY 1986-87. The predecessor to CCD was the Elementary and Secondary General…

Proton radiation damage experiment on P-Channel CCD for an X-ray CCD camera onboard the ASTRO-H satellite

NASA Astrophysics Data System (ADS)

Mori, Koji; Nishioka, Yusuke; Ohura, Satoshi; Koura, Yoshiaki; Yamauchi, Makoto; Nakajima, Hiroshi; Ueda, Shutaro; Kan, Hiroaki; Anabuki, Naohisa; Nagino, Ryo; Hayashida, Kiyoshi; Tsunemi, Hiroshi; Kohmura, Takayoshi; Ikeda, Shoma; Murakami, Hiroshi; Ozaki, Masanobu; Dotani, Tadayasu; Maeda, Yukie; Sagara, Kenshi

2013-12-01

We report on a proton radiation damage experiment on P-channel CCD newly developed for an X-ray CCD camera onboard the ASTRO-H satellite. The device was exposed up to 109 protons cm-2 at 6.7 MeV. The charge transfer inefficiency (CTI) was measured as a function of radiation dose. In comparison with the CTI currently measured in the CCD camera onboard the Suzaku satellite for 6 years, we confirmed that the new type of P-channel CCD is radiation tolerant enough for space use. We also confirmed that a charge-injection technique and lowering the operating temperature efficiently work to reduce the CTI for our device. A comparison with other P-channel CCD experiments is also discussed. We performed a proton radiation damage experiment on a new P-channel CCD. The device was exposed up to 109 protons cm-2 at 6.7 MeV. We confirmed that it is radiation tolerant enough for space use. We confirmed that a charge-injection technique reduces the CTI. We confirmed that lowering the operating temperature also reduces the CTI.

Photometric and spectrophotometric activity of P/Halley during 1984-85

NASA Technical Reports Server (NTRS)

Barker, E. S.; Opal, C. B.

1986-01-01

Imaging and spectroscopic observations of P/Halley were performed during 1984 and 1985 using a CCD camera and an Image Dissector Scanner Spectrograph, and the results are reported. P/Halley activity appears to begin around 6.23 AU and varies as r exp -5.3 for heliocentric distances between 5.8 and 4.2 AU. The observed radial brightness distribution falls off much faster than the inverse of the projected distance from the nucleus, indicating that the particles are disappearing or evaporating within about 20,000 km of the nucleus. Weak CN emission was detected in spectra obtained during February 1985, corresponding to a column density of 3 x 10 to the 8th molecules/sq cm. The spectroscopic results indicate that the excess of the observed emission over that from the bare nucleus is dominated by scattered solar continuum from grains, rather than by molecular coma emissions.

Optimal parallel solution of sparse triangular systems

NASA Technical Reports Server (NTRS)

Alvarado, Fernando L.; Schreiber, Robert

1990-01-01

A method for the parallel solution of triangular sets of equations is described that is appropriate when there are many right-handed sides. By preprocessing, the method can reduce the number of parallel steps required to solve Lx = b compared to parallel forward or backsolve. Applications are to iterative solvers with triangular preconditioners, to structural analysis, or to power systems applications, where there may be many right-handed sides (not all available a priori). The inverse of L is represented as a product of sparse triangular factors. The problem is to find a factored representation of this inverse of L with the smallest number of factors (or partitions), subject to the requirement that no new nonzero elements be created in the formation of these inverse factors. A method from an earlier reference is shown to solve this problem. This method is improved upon by constructing a permutation of the rows and columns of L that preserves triangularity and allow for the best possible such partition. A number of practical examples and algorithmic details are presented. The parallelism attainable is illustrated by means of elimination trees and clique trees.

Chromatography of blood-clotting factors and serum proteins on columns of diatomaceous earth.

PubMed

MILSTONE, J H

1955-07-20

1. In batch adsorptions with prothrombin solutions, hyflo was the weakest adsorbent, standard super-cel intermediate, and filter-cel strongest. Of these three grades of diatomaceous earth, hyflo has the smallest surface area per gram and filter-cel the largest. In parallel breakthrough experiments, a column of standard super-cel had a capacity almost six times that of a hyflo column. 2. After partial removal of impurities by diatomaceous earth, prothrombin preparations contained less thrombokinase, were more stable, and displayed less tendency to form thrombin "spontaneously." Thrombokinase (or its precursor) was removed from a preparation of prothrombin by passage through a filter cake of standard super-cel. The specific activity of the prothrombin was increased; and 62 per cent of the activity was recovered. 3. Prothrombin was adsorbed from an ammonium sulfate solution at pH 5.26 by columns of hyflo or standard super-cel. When eluted by phosphate solutions, the protein moved down the columns more readily at higher pH and higher concentration of phosphate salts, within the pH range 5.0 to 6.6, and within the phosphate range 0.1 to 1.0 M. 4. Thrombin was adsorbed on a column of standard super-cel at pH 5.11. As successive eluents passed through the column, the thrombin emerged between two bands of impurities. The specific activity of the thrombin was raised; and 83 per cent of the activity was recovered. 5. With a column of standard super-cel, and with a series of eluents within the pH range 5.1 to 6.3, total serum proteins were separated into four major bands. About 94 per cent of the protein was recovered.

CHROMATOGRAPHY OF BLOOD-CLOTTING FACTORS AND SERUM PROTEINS ON COLUMNS OF DIATOMACEOUS EARTH

PubMed Central

Milstone, J. H.

1955-01-01

1. In batch adsorptions with prothrombin solutions, hyflo was the weakest adsorbent, standard super-cel intermediate, and filter-cel strongest. Of these three grades of diatomaceous earth, hyflo has the smallest surface area per gram and filter-cel the largest. In parallel breakthrough experiments, a column of standard super-cel had a capacity almost six times that of a hyflo column. 2. After partial removal of impurities by diatomaceous earth, prothrombin preparations contained less thrombokinase, were more stable, and displayed less tendency to form thrombin "spontaneously." Thrombokinase (or its precursor) was removed from a preparation of prothrombin by passage through a filter cake of standard super-cel. The specific activity of the prothrombin was increased; and 62 per cent of the activity was recovered. 3. Prothrombin was adsorbed from an ammonium sulfate solution at pH 5.26 by columns of hyflo or standard super-cel. When eluted by phosphate solutions, the protein moved down the columns more readily at higher pH and higher concentration of phosphate salts, within the pH range 5.0 to 6.6, and within the phosphate range 0.1 to 1.0 M. 4. Thrombin was adsorbed on a column of standard super-cel at pH 5.11. As successive eluents passed through the column, the thrombin emerged between two bands of impurities. The specific activity of the thrombin was raised; and 83 per cent of the activity was recovered. 5. With a column of standard super-cel, and with a series of eluents within the pH range 5.1 to 6.3, total serum proteins were separated into four major bands. About 94 per cent of the protein was recovered. PMID:13242761

Two-dimensional radiant energy array computers and computing devices

NASA Technical Reports Server (NTRS)

Schaefer, D. H.; Strong, J. P., III (Inventor)

1976-01-01

Two dimensional digital computers and computer devices operate in parallel on rectangular arrays of digital radiant energy optical signal elements which are arranged in ordered rows and columns. Logic gate devices receive two input arrays and provide an output array having digital states dependent only on the digital states of the signal elements of the two input arrays at corresponding row and column positions. The logic devices include an array of photoconductors responsive to at least one of the input arrays for either selectively accelerating electrons to a phosphor output surface, applying potentials to an electroluminescent output layer, exciting an array of discrete radiant energy sources, or exciting a liquid crystal to influence crystal transparency or reflectivity.

CCD correlation techniques

NASA Technical Reports Server (NTRS)

Hewes, C. R.; Bosshart, P. W.; Eversole, W. L.; Dewit, M.; Buss, D. D.

1976-01-01

Two CCD techniques were discussed for performing an N-point sampled data correlation between an input signal and an electronically programmable reference function. The design and experimental performance of an implementation of the direct time correlator utilizing two analog CCDs and MOS multipliers on a single IC were evaluated. The performance of a CCD implementation of the chirp z transform was described, and the design of a new CCD integrated circuit for performing correlation by multiplication in the frequency domain was presented. This chip provides a discrete Fourier transform (DFT) or inverse DFT, multipliers, and complete support circuitry for the CCD CZT. The two correlation techniques are compared.

Scalable Track Detection in SAR CCD Images

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chow, James G; Quach, Tu-Thach

Existing methods to detect vehicle tracks in coherent change detection images, a product of combining two synthetic aperture radar images ta ken at different times of the same scene, rely on simple, fast models to label track pixels. These models, however, are often too simple to capture natural track features such as continuity and parallelism. We present a simple convolutional network architecture consisting of a series of 3-by-3 convolutions to detect tracks. The network is trained end-to-end to learn natural track features entirely from data. The network is computationally efficient and improves the F-score on a standard dataset to 0.988,more » up fr om 0.907 obtained by the current state-of-the-art method.« less

30-lens interferometer for high energy x-rays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lyubomirskiy, M., E-mail: lyubomir@esrf.fr; Snigireva, I., E-mail: irina@esrf.fr; Vaughan, G.

2016-07-27

We report a hard X-ray multilens interferometer consisting of 30 parallel compound refractive lenses. Under coherent illumination each CRL creates a diffraction limited focal spot - secondary source. An overlapping of coherent beams from these sources resulting in the interference pattern which has a rich longitudinal structure in accordance with the Talbot imaging formalism. The proposed interferometer was experimentally tested at ID11 ESRF beamline for the photon energies 32 keV and 65 keV. The fundamental and fractional Talbot images were recorded with the high resolution CCD camera. An effective source size in the order of 15 µm was determined frommore » the first Talbot image proving that the multilens interferometer can be used as a high resolution beam diagnostic tool.« less

A safety monitoring system for taxi based on CMOS imager

NASA Astrophysics Data System (ADS)

Liu, Zhi

2005-01-01

CMOS image sensors now become increasingly competitive with respect to their CCD counterparts, while adding advantages such as no blooming, simpler driving requirements and the potential of on-chip integration of sensor, analogue circuitry, and digital processing functions. A safety monitoring system for taxi based on cmos imager that can record field situation when unusual circumstance happened is described in this paper. The monitoring system is based on a CMOS imager (OV7120), which can output digital image data through parallel pixel data port. The system consists of a CMOS image sensor, a large capacity NAND FLASH ROM, a USB interface chip and a micro controller (AT90S8515). The structure of whole system and the test data is discussed and analyzed in detail.

Stabilization of the Virulence Plasmid pSLT of Salmonella Typhimurium by Three Maintenance Systems and Its Evaluation by Using a New Stability Test.

PubMed

Lobato-Márquez, Damián; Molina-García, Laura; Moreno-Córdoba, Inma; García-Del Portillo, Francisco; Díaz-Orejas, Ramón

2016-01-01

Certain Salmonella enterica serovars belonging to subspecies I carry low-copy-number virulence plasmids of variable size (50-90 kb). All of these plasmids share the spv operon, which is important for systemic infection. Virulence plasmids are present at low copy numbers. Few copies reduce metabolic burden but suppose a risk of plasmid loss during bacterial division. This drawback is counterbalanced by maintenance modules that ensure plasmid stability, including partition systems and toxin-antitoxin (TA) loci. The low-copy number virulence pSLT plasmid of Salmonella enterica serovar Typhimurium encodes three auxiliary maintenance systems: one partition system ( parAB ) and two TA systems ( ccdAB ST and vapBC2 ST ). The TA module ccdAB ST has previously been shown to contribute to pSLT plasmid stability and vapBC2 ST to bacterial virulence. Here we describe a novel assay to measure plasmid stability based on the selection of plasmid-free cells following elimination of plasmid-containing cells by ParE toxin, a DNA gyrase inhibitor. Using this new maintenance assay we confirmed a crucial role of parAB in pSLT maintenance. We also showed that vapBC2 ST , in addition to contribute to bacterial virulence, is important for plasmid stability. We have previously shown that ccdAB ST encodes an inactive CcdB ST toxin. Using our new stability assay we monitored the contribution to plasmid stability of a ccdAB ST variant containing a single mutation (R99W) that restores the toxicity of CcdB ST . The "activation" of CcdB ST (R99W) did not increase pSLT stability by ccdAB ST . In contrast, ccdAB ST behaves as a canonical type II TA system in terms of transcriptional regulation. Of interest, ccdAB ST was shown to control the expression of a polycistronic operon in the pSLT plasmid. Collectively, these results show that the contribution of the CcdB ST toxin to pSLT plasmid stability may depend on its role as a co-repressor in coordination with CcdA ST antitoxin more than on its toxic activity.

Spinally projecting neurons of the dorsal column nucleus in a reptile: locus of origin and trajectory of termination.

PubMed

Pritz, M B

1996-01-01

Interconnections between the dorsal column nucleus and the spinal cord were investigated in a reptile, Caiman crocodilus. After placement of an anterograde tracer into the dorsal column nucleus, descending fibers are seen to leave this nucleus to enter the dorsal funiculus where they course ventrally to terminate in lamina V of the spinal cord as far caudally as C2. Placement of a retrograde tracer into cut fibers of the cervical spinal cord identified the relay cells of the dorsal column nucleus that project to the spinal cord. These neurons were mainly clustered in a caudal and ventral part of this nucleus. The soma of these spinally projecting cells were small and were generally round or oval in shape. A number of these neurons had the long axis of their soma oriented dorsoventrally, with a primary dendrite extending dorsally. Fibers in the dorsal funiculus that originated from the spinal cord enter the caudal part of the dorsal column nucleus and turn ventral. In the dorsal column nucleus, these axons run parallel to the vertically oriented dendrites of these spinally projecting cells before termination in close relation to the cell bodies of these neurons. Quantitative observations (mean +/- standard error) were made on well labeled neurons and included several measurements: area, perimeter, and degree of eccentricity (greatest width/greatest length) in both the transverse as well as the sagittal plane. These spinally projecting neurons in Caiman are located in the dorsal column nucleus in a position similar to that of spinally projecting cells in cats.

CCD detector development projects by the Beamline Technical Support Group at the Advanced Photon Source

NASA Astrophysics Data System (ADS)

Lee, John H.; Fernandez, Patricia; Madden, Tim; Molitsky, Michael; Weizeorick, John

2007-11-01

This paper will describe two ongoing detector projects being developed by the Beamline Technical Support Group at the Advanced Photon Source (APS) at Argonne National Laboratory (ANL). The first project is the design and construction of two detectors: a single-CCD system and a two-by-two Mosaic CCD camera for Small-Angle X-ray Scattering (SAXS). Both of these systems utilize the Kodak KAF-4320E CCD coupled to fiber optic tapers, custom mechanical hardware, electronics, and software developed at ANL. The second project is a Fast-CCD (FCCD) detector being developed in a collaboration between ANL and Lawrence Berkeley National Laboratory (LBNL). This detector will use ANL-designed readout electronics and a custom LBNL-designed CCD, with 480×480 pixels and 96 outputs, giving very fast readout.

The In-flight Spectroscopic Performance of the Swift XRT CCD Camera During 2006-2007

NASA Technical Reports Server (NTRS)

Godet, O.; Beardmore, A.P.; Abbey, A.F.; Osborne, J.P.; Page, K.L.; Evans, P.; Starling, R.; Wells, A.A.; Angelini, L.; Burrows, D.N.;

Pathogen webs in collapsing honey bee colonies.

PubMed

Cornman, R Scott; Tarpy, David R; Chen, Yanping; Jeffreys, Lacey; Lopez, Dawn; Pettis, Jeffery S; vanEngelsdorp, Dennis; Evans, Jay D

2012-01-01

Recent losses in honey bee colonies are unusual in their severity, geographical distribution, and, in some cases, failure to present recognized characteristics of known disease. Domesticated honey bees face numerous pests and pathogens, tempting hypotheses that colony collapses arise from exposure to new or resurgent pathogens. Here we explore the incidence and abundance of currently known honey bee pathogens in colonies suffering from Colony Collapse Disorder (CCD), otherwise weak colonies, and strong colonies from across the United States. Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies. Pathogen loads were highly covariant in CCD but not control hives, suggesting that CCD colonies rapidly become susceptible to a diverse set of pathogens, or that co-infections can act synergistically to produce the rapid depletion of workers that characterizes the disorder. We also tested workers from a CCD-free apiary to confirm that significant positive correlations among pathogen loads can develop at the level of individual bees and not merely as a secondary effect of CCD. This observation and other recent data highlight pathogen interactions as important components of bee disease. Finally, we used deep RNA sequencing to further characterize microbial diversity in CCD and non-CCD hives. We identified novel strains of the recently described Lake Sinai viruses (LSV) and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD. The results are discussed with respect to host-parasite interactions and other environmental stressors of honey bees.

Modeling the impact of preflushing on CTE in proton irradiated CCD-based detectors

NASA Astrophysics Data System (ADS)

Philbrick, R. H.

2002-04-01

A software model is described that performs a "real world" simulation of the operation of several types of charge-coupled device (CCD)-based detectors in order to accurately predict the impact that high-energy proton radiation has on image distortion and modulation transfer function (MTF). The model was written primarily to predict the effectiveness of vertical preflushing on the custom full frame CCD-based detectors intended for use on the proposed Kepler Discovery mission, but it is capable of simulating many other types of CCD detectors and operating modes as well. The model keeps track of the occupancy of all phosphorous-silicon (P-V), divacancy (V-V) and oxygen-silicon (O-V) defect centers under every CCD electrode over the entire detector area. The integrated image is read out by simulating every electrode-to-electrode charge transfer in both the vertical and horizontal CCD registers. A signal level dependency on the capture and emission of signal is included and the current state of each electrode (e.g., barrier or storage) is considered when distributing integrated and emitted signal. Options for performing preflushing, preflashing, and including mini-channels are available on both the vertical and horizontal CCD registers. In addition, dark signal generation and image transfer smear can be selectively enabled or disabled. A comparison of the charge transfer efficiency (CTE) data measured on the Hubble space telescope imaging spectrometer (STIS) CCD with the CTE extracted from model simulations of the STIS CCD show good agreement.

Characterization of Electrically Active Defects in Si Using CCD Image Sensors

DTIC Science & Technology

1978-02-01

63 35 Dislocation Segments in CCD Imager . . . . . . . . . . . . . 64 36 422 Reflection Topograph of Dislocation Loop ir... Loops . . . . . 3 39 422 Reflection Topograph of Scratch on CCD Imager, . . . 69 40 Dark Current Display of a CCD Imager with 32 ms integration Time...made of each slice using the elon -asoorbio aold developer described in Appendix D. The inagers were then thinned using the procedure at Appendix taor

Mosaic CCD method: A new technique for observing dynamics of cometary magnetospheres

NASA Technical Reports Server (NTRS)

Saito, T.; Takeuchi, H.; Kozuba, Y.; Okamura, S.; Konno, I.; Hamabe, M.; Aoki, T.; Minami, S.; Isobe, S.

1992-01-01

On April 29, 1990, the plasma tail of Comet Austin was observed with a CCD camera on the 105-cm Schmidt telescope at the Kiso Observatory of the University of Tokyo. The area of the CCD used in this observation is only about 1 sq cm. When this CCD is used on the 105-cm Schmidt telescope at the Kiso Observatory, the area corresponds to a narrow square view of 12 ft x 12 ft. By comparison with the photograph of Comet Austin taken by Numazawa (personal communication) on the same night, we see that only a small part of the plasma tail can be photographed at one time with the CCD. However, by shifting the view on the CCD after each exposure, we succeeded in imaging the entire length of the cometary magnetosphere of 1.6 x 10(exp 6) km. This new technique is called 'the mosaic CCD method'. In order to study the dynamics of cometary plasma tails, seven frames of the comet from the head to the tail region were twice imaged with the mosaic CCD method and two sets of images were obtained. Six microstructures, including arcade structures, were identified in both the images. Sketches of the plasma tail including microstructures are included.

Genetic mapping of the cleidocranial dysplasia (CCD) locus on chromosome band 6p21 to include a microdeletion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gelb, B.D.; Desnick, R.J.; Shevell, M.

1995-08-28

Cleidocranial dysplasia (CCD) is a generalized skeletal dysplasia with autosomal dominant inheritance. Recently, the CCD disease locus was localized to 23 and 17 cM regions of chromosome band 6p21 by linkage studies of seven affected families. Of note, the 23 cM region contained a microdeletion detected in one family at D6S459, an interval that was excluded in the 17 cM overlapping region. Here, linkage of CCD to 6p21 was independently confirmed with a maximal two-point LOD score of Z=5.12 with marker D6S452 at {theta}=0.00. Recombinant events in two affected individuals defined a CCD region of 7 cM from D6S465 tomore » D6S282, which overlapped with the CCD region containing the microdeletion but did not overlap with the 17 cM critical region from D6S282 to D6S291. These results suggest the refined localization of the CCD region to 6 cM spanning markers D6S438 to D6S282, thereby reviving the possibility that the CCD gene lies within the microdeletion at D6S459. 13 refs., 2 figs., 1 tab.« less

CCD radiation damage in ESA Cosmic Visions missions: assessment and mitigation

NASA Astrophysics Data System (ADS)

Lumb, David H.

2009-08-01

Charge Coupled Device (CCD) imagers have been widely used in space-borne astronomical instruments. A frequent concern has been the radiation damage effects on the CCD charge transfer properties. We review some methods for assessing the Charge Transfer Inefficiency (CTI) in CCDs. Techniques to minimise degradation using background charge injection and p-channel CCD architectures are discussed. A critical review of the claims for p-channel architectures is presented. The performance advantage for p-channel CCD performance is shown to be lower than claimed previously. Finally we present some projections for the performance in the context of some future ESA missions.

High-performance ultra-low power VLSI analog processor for data compression

NASA Technical Reports Server (NTRS)

Tawel, Raoul (Inventor)

1996-01-01

An apparatus for data compression employing a parallel analog processor. The apparatus includes an array of processor cells with N columns and M rows wherein the processor cells have an input device, memory device, and processor device. The input device is used for inputting a series of input vectors. Each input vector is simultaneously input into each column of the array of processor cells in a pre-determined sequential order. An input vector is made up of M components, ones of which are input into ones of M processor cells making up a column of the array. The memory device is used for providing ones of M components of a codebook vector to ones of the processor cells making up a column of the array. A different codebook vector is provided to each of the N columns of the array. The processor device is used for simultaneously comparing the components of each input vector to corresponding components of each codebook vector, and for outputting a signal representative of the closeness between the compared vector components. A combination device is used to combine the signal output from each processor cell in each column of the array and to output a combined signal. A closeness determination device is then used for determining which codebook vector is closest to an input vector from the combined signals, and for outputting a codebook vector index indicating which of the N codebook vectors was the closest to each input vector input into the array.

The dorsal tectal longitudinal column (TLCd): a second longitudinal column in the paramedian region of the midbrain tectum.

PubMed

Aparicio, M-Auxiliadora; Saldaña, Enrique

2014-03-01

The tectal longitudinal column (TLC) is a longitudinally oriented, long and narrow nucleus that spans the paramedian region of the midbrain tectum of a large variety of mammals (Saldaña et al. in J Neurosci 27:13108-13116, 2007). Recent analysis of the organization of this region revealed another novel nucleus located immediately dorsal, and parallel, to the TLC. Because the name "tectal longitudinal column" also seems appropriate for this novel nucleus, we suggest the TLC described in 2007 be renamed the "ventral tectal longitudinal column (TLCv)", and the newly discovered nucleus termed the "dorsal tectal longitudinal column (TLCd)". This work represents the first characterization of the rat TLCd. A constellation of anatomical techniques was used to demonstrate that the TLCd differs from its surrounding structures (TLCv and superior colliculus) cytoarchitecturally, myeloarchitecturally, neurochemically and hodologically. The distinct expression of vesicular amino acid transporters suggests that TLCd neurons are GABAergic. The TLCd receives major projections from various areas of the cerebral cortex (secondary visual mediomedial area, and granular and dysgranular retrosplenial cortices) and from the medial pretectal nucleus. It densely innervates the ipsilateral lateral posterior and laterodorsal nuclei of the thalamus. Thus, the TLCd is connected with vision-related neural centers. The TLCd may be unique as it constitutes the only known nucleus made of GABAergic neurons dedicated to providing massive inhibition to higher order thalamic nuclei of a specific sensory modality.

Quantitative evaluation of the accuracy and variance of individual pixels in a scientific CMOS (sCMOS) camera for computational imaging

NASA Astrophysics Data System (ADS)

Watanabe, Shigeo; Takahashi, Teruo; Bennett, Keith

2017-02-01

The"scientific" CMOS (sCMOS) camera architecture fundamentally differs from CCD and EMCCD cameras. In digital CCD and EMCCD cameras, conversion from charge to the digital output is generally through a single electronic chain, and the read noise and the conversion factor from photoelectrons to digital outputs are highly uniform for all pixels, although quantum efficiency may spatially vary. In CMOS cameras, the charge to voltage conversion is separate for each pixel and each column has independent amplifiers and analog-to-digital converters, in addition to possible pixel-to-pixel variation in quantum efficiency. The "raw" output from the CMOS image sensor includes pixel-to-pixel variability in the read noise, electronic gain, offset and dark current. Scientific camera manufacturers digitally compensate the raw signal from the CMOS image sensors to provide usable images. Statistical noise in images, unless properly modeled, can introduce errors in methods such as fluctuation correlation spectroscopy or computational imaging, for example, localization microscopy using maximum likelihood estimation. We measured the distributions and spatial maps of individual pixel offset, dark current, read noise, linearity, photoresponse non-uniformity and variance distributions of individual pixels for standard, off-the-shelf Hamamatsu ORCA-Flash4.0 V3 sCMOS cameras using highly uniform and controlled illumination conditions, from dark conditions to multiple low light levels between 20 to 1,000 photons / pixel per frame to higher light conditions. We further show that using pixel variance for flat field correction leads to errors in cameras with good factory calibration.

Targeting excited states in all-trans polyenes with electron-pair states.

PubMed

Boguslawski, Katharina

2016-12-21

Wavefunctions restricted to electron pair states are promising models for strongly correlated systems. Specifically, the pair Coupled Cluster Doubles (pCCD) ansatz allows us to accurately describe bond dissociation processes and heavy-element containing compounds with multiple quasi-degenerate single-particle states. Here, we extend the pCCD method to model excited states using the equation of motion (EOM) formalism. As the cluster operator of pCCD is restricted to electron-pair excitations, EOM-pCCD allows us to target excited electron-pair states only. To model singly excited states within EOM-pCCD, we modify the configuration interaction ansatz of EOM-pCCD to contain also single excitations. Our proposed model represents a simple and cost-effective alternative to conventional EOM-CC methods to study singly excited electronic states. The performance of the excited state models is assessed against the lowest-lying excited states of the uranyl cation and the two lowest-lying excited states of all-trans polyenes. Our numerical results suggest that EOM-pCCD including single excitations is a good starting point to target singly excited states.

Parallel robot for micro assembly with integrated innovative optical 3D-sensor

NASA Astrophysics Data System (ADS)

Hesselbach, Juergen; Ispas, Diana; Pokar, Gero; Soetebier, Sven; Tutsch, Rainer

2002-10-01

Recent advances in the fields of MEMS and MOEMS often require precise assembly of very small parts with an accuracy of a few microns. In order to meet this demand, a new approach using a robot based on parallel mechanisms in combination with a novel 3D-vision system has been chosen. The planar parallel robot structure with 2 DOF provides a high resolution in the XY-plane. It carries two additional serial axes for linear and rotational movement in/about z direction. In order to achieve high precision as well as good dynamic capabilities, the drive concept for the parallel (main) axes incorporates air bearings in combination with a linear electric servo motors. High accuracy position feedback is provided by optical encoders with a resolution of 0.1 μm. To allow for visualization and visual control of assembly processes, a camera module fits into the hollow tool head. It consists of a miniature CCD camera and a light source. In addition a modular gripper support is integrated into the tool head. To increase the accuracy a control loop based on an optoelectronic sensor will be implemented. As a result of an in-depth analysis of different approaches a photogrammetric system using one single camera and special beam-splitting optics was chosen. A pattern of elliptical marks is applied to the surfaces of workpiece and gripper. Using a model-based recognition algorithm the image processing software identifies the gripper and the workpiece and determines their relative position. A deviation vector is calculated and fed into the robot control to guide the gripper.

Multi-arm spectrometer for parallel frequency analysis of radio-wave signals oriented to astronomical observations

NASA Astrophysics Data System (ADS)

Shcherbakov, Alexandre S.; Chavez Dagostino, Miguel; Arellanes, Adan Omar; Tepichin Rodriguez, Eduardo

2017-08-01

We describe a potential prototype of modern spectrometer based on acousto-optical technique with three parallel optical arms for analysis of radio-wave signals specific to astronomical observations. Each optical arm exhibits original performances to provide parallel multi-band observations with different scales simultaneously. Similar multi-band instrument is able to realize measurements within various scenarios from planetary atmospheres to attractive objects in the distant Universe. The arrangement under development has two novelties. First, each optical arm represents an individual spectrum analyzer with its individual performances. Such an approach is conditioned by exploiting various materials for acousto-optical cells operating within various regimes, frequency ranges, and light wavelengths from independent light sources. Individually produced beam shapers give both the needed incident light polarization and the required apodization for light beam to increase the dynamic range of the system as a whole. After parallel acousto-optical processing, a few data flows from these optical arms are united by the joint CCD matrix on the stage of the combined extremely high-bit rate electronic data processing that provides the system performances as well. The other novelty consists in the usage of various materials for designing wide-aperture acousto-optical cells exhibiting the best performances within each of optical arms. Here, one can mention specifically selected cuts of tellurium dioxide, bastron, and lithium niobate, which overlap selected areas within the frequency range from 40 MHz to 2.0 GHz. Thus one yields the united versatile instrument for comprehensive studies of astronomical objects simultaneously with precise synchronization in various frequency ranges.

Real-time Full-spectral Imaging and Affinity Measurements from 50 Microfluidic Channels using Nanohole Surface Plasmon Resonance†

PubMed Central

Lee, Si Hoon; Lindquist, Nathan C.; Wittenberg, Nathan J.; Jordan, Luke R.; Oh, Sang-Hyun

2012-01-01

With recent advances in high-throughput proteomics and systems biology, there is a growing demand for new instruments that can precisely quantify a wide range of receptor-ligand binding kinetics in a high-throughput fashion. Here we demonstrate a surface plasmon resonance (SPR) imaging spectroscopy instrument capable of extracting binding kinetics and affinities from 50 parallel microfluidic channels simultaneously. The instrument utilizes large-area (~cm2) metallic nanohole arrays as SPR sensing substrates and combines a broadband light source, a high-resolution imaging spectrometer and a low-noise CCD camera to extract spectral information from every channel in real time with a refractive index resolution of 7.7 × 10−6. To demonstrate the utility of our instrument for quantifying a wide range of biomolecular interactions, each parallel microfluidic channel is coated with a biomimetic supported lipid membrane containing ganglioside (GM1) receptors. The binding kinetics of cholera toxin b (CTX-b) to GM1 are then measured in a single experiment from 50 channels. By combining the highly parallel microfluidic device with large-area periodic nanohole array chips, our SPR imaging spectrometer system enables high-throughput, label-free, real-time SPR biosensing, and its full-spectral imaging capability combined with nanohole arrays could enable integration of SPR imaging with concurrent surface-enhanced Raman spectroscopy. PMID:22895607

Method and apparatus for white-light dispersed-fringe interferometric measurement of corneal topography

NASA Technical Reports Server (NTRS)

Hochberg, Eric B. (Inventor); Baroth, Edmund C. (Inventor)

1994-01-01

An novel interferometric apparatus and method for measuring the topography of aspheric surfaces, without requiring any form of scanning or phase shifting. The apparatus and method of the present invention utilize a white-light interferometer, such as a white-light Twyman-Green interferometer, combined with a means for dispersing a polychromatic interference pattern, using a fiber-optic bundle and a disperser such as a prism for determining the monochromatic spectral intensities of the polychromatic interference pattern which intensities uniquely define the optical path differences or OPD between the surface under test and a reference surface such as a reference sphere. Consequently, the present invention comprises a snapshot approach to measuring aspheric surface topographies such as the human cornea, thereby obviating vibration sensitive scanning which would otherwise reduce the accuracy of the measurement. The invention utilizes a polychromatic interference pattern in the pupil image plane, which is dispersed on a point-wise basis, by using a special area-to-line fiber-optic manifold, onto a CCD or other type detector comprising a plurality of columns of pixels. Each such column is dedicated to a single point of the fringe pattern for enabling determination of the spectral content of the pattern. The auto-correlation of the dispersed spectrum of the fringe pattern is uniquely characteristic of a particular optical path difference between the surface under test and a reference surface.

Fixed-bed column performances of azure-II and auramine-O adsorption by Pinus eldarica stalks activated carbon and its composite with zno nanoparticles: Optimization by response surface methodology based on central composite design.

PubMed

Jafari, Maryam; Rahimi, Mahmood Reza; Ghaedi, Mehrorang; Javadian, Hamedreza; Asfaram, Arash

2017-12-01

A continuous adsorption was used for removal of azure II (AZ II) and auramine O (AO) from aqueous solutions using Pinus eldarica stalks activated carbon (PES-AC) from aqueous solutions. The effects of initial dye concentration, flow rate, bed height and contact time on removal percentage of AO and AZ II were evaluated and optimized by central composite design (CCD) at optimum pH = 7.0. ZnO nanoparticles loaded on activated carbon were also used to remove AO and AZ II at pH = 7.0 and other optimum conditions. The breakthrough curves were obtained at different flow rates, initial dye concentrations and bed heights and the experimental data were fitted by Thomas, Adams-Bohart and Yoon-Nelson models. The main parameters of fixed-bed column including its adsorption capacity at breakthrough point (q b ), adsorption capacity at saturation point (q s ), mass transfer zone (MTZ), total removal percentage (R%), and empty bed contact time (EBCT) were calculated. The removal percentages calculated for AZ II and AO II were in the range of 51.6-61.1% and 40.6-61.6%, respectively. Bed adsorption capacity (N 0 ) and critical bed depth (Z 0 ) were obtained by BDST model. Copyright © 2017 Elsevier Inc. All rights reserved.

Environmental Conditions in a Carpathian Deep Sea Basin During the Period Preceding Oceanic Anoxic Event 2 - A Case Study from the Skole Nappe

NASA Astrophysics Data System (ADS)

Bąk, Krzysztof; Bąk, Marta; Górny, Zbigniew; Wolska, Anna

2015-01-01

Hemipelagic green clayey shales and thin muddy turbidites accumulated in a deep sea environment below the CCD in the Skole Basin, a part of the Outer Carpathian realm, during the Middle Cenomanian. The hemipelagites contain numerous radiolarians, associated with deep-water agglutinated foraminifera. These sediments accumulated under mesotrophic conditions with limited oxygen concentration. Short-term periodic anoxia also occurred during that time. Muddy turbidity currents caused deposition of siliciclastic and biogenic material, including calcareous foramini-fers and numerous sponge spicules. The preservation and diversity of the spicules suggests that they originate from disarticulation of moderately diversified sponge assemblages, which lived predominantly in the neritic-bathyal zone. Analyses of radiolarian ecological groups and pellets reflect the water column properties during the sedimentation of green shales. At that time, surface and also intermediate waters were oxygenated enough and sufficiently rich in nutri-ents to enable plankton production. Numerous, uncompacted pellets with nearly pristine radiolarian skeletons inside show that pelletization was the main factor of radiolarian flux into the deep basin floor. Partly dissolved skeletons indicate that waters in the Skole Basin were undersaturated in relation to silica content. Oxygen content might have been depleted in the deeper part of the water column causing periodic anoxic conditions which prevent rapid bacterial degra-dation of the pellets during their fall to the sea floor.

A 12-bit high-speed column-parallel two-step single-slope analog-to-digital converter (ADC) for CMOS image sensors.

PubMed

Lyu, Tao; Yao, Suying; Nie, Kaiming; Xu, Jiangtao

2014-11-17

A 12-bit high-speed column-parallel two-step single-slope (SS) analog-to-digital converter (ADC) for CMOS image sensors is proposed. The proposed ADC employs a single ramp voltage and multiple reference voltages, and the conversion is divided into coarse phase and fine phase to improve the conversion rate. An error calibration scheme is proposed to correct errors caused by offsets among the reference voltages. The digital-to-analog converter (DAC) used for the ramp generator is based on the split-capacitor array with an attenuation capacitor. Analysis of the DAC's linearity performance versus capacitor mismatch and parasitic capacitance is presented. A prototype 1024 × 32 Time Delay Integration (TDI) CMOS image sensor with the proposed ADC architecture has been fabricated in a standard 0.18 μm CMOS process. The proposed ADC has average power consumption of 128 μW and a conventional rate 6 times higher than the conventional SS ADC. A high-quality image, captured at the line rate of 15.5 k lines/s, shows that the proposed ADC is suitable for high-speed CMOS image sensors.

CFCCD Manual | CTIO

Science.gov Websites

DECam SAM 0.9-m CCD Goodman SOI Optical Spectrographs CHIRON COSMOS Goodman Filters Telescopes Blanco 4 4.4.4 Gain 4.5: CCD scales at various foci APPENDIX I: Filters for CCD Imaging II: Gain and Readout

High-frame rate multiport CCD imager and camera

NASA Astrophysics Data System (ADS)

Levine, Peter A.; Patterson, David R.; Esposito, Benjamin J.; Tower, John R.; Lawler, William B.

1993-01-01

A high frame rate visible CCD camera capable of operation up to 200 frames per second is described. The camera produces a 256 X 256 pixel image by using one quadrant of a 512 X 512 16-port, back illuminated CCD imager. Four contiguous outputs are digitally reformatted into a correct, 256 X 256 image. This paper details the architecture and timing used for the CCD drive circuits, analog processing, and the digital reformatter.

Lack of evidence for an association between Iridovirus and colony collapse disorder.

PubMed

Tokarz, Rafal; Firth, Cadhla; Street, Craig; Cox-Foster, Diana L; Lipkin, W Ian

2011-01-01

Colony collapse disorder (CCD) is characterized by the unexplained losses of large numbers of adult worker bees (Apis mellifera) from apparently healthy colonies. Although infections, toxins, and other stressors have been associated with the onset of CCD, the pathogenesis of this disorder remains obscure. Recently, a proteomics study implicated a double-stranded DNA virus, invertebrate iridescent virus (Family Iridoviridae) along with a microsporidium (Nosema sp.) as the cause of CCD. We tested the validity of this relationship using two independent methods: (i) we surveyed healthy and CCD colonies from the United States and Israel for the presence of members of the Iridovirus genus and (ii) we reanalyzed metagenomics data previously generated from RNA pools of CCD colonies for the presence of Iridovirus-like sequences. Neither analysis revealed any evidence to suggest the presence of an Iridovirus in healthy or CCD colonies.

Lack of Evidence for an Association between Iridovirus and Colony Collapse Disorder

PubMed Central

Street, Craig; Cox-Foster, Diana L.; Lipkin, W. Ian

2011-01-01

Colony collapse disorder (CCD) is characterized by the unexplained losses of large numbers of adult worker bees (Apis mellifera) from apparently healthy colonies. Although infections, toxins, and other stressors have been associated with the onset of CCD, the pathogenesis of this disorder remains obscure. Recently, a proteomics study implicated a double-stranded DNA virus, invertebrate iridescent virus (Family Iridoviridae) along with a microsporidium (Nosema sp.) as the cause of CCD. We tested the validity of this relationship using two independent methods: (i) we surveyed healthy and CCD colonies from the United States and Israel for the presence of members of the Iridovirus genus and (ii) we reanalyzed metagenomics data previously generated from RNA pools of CCD colonies for the presence of Iridovirus-like sequences. Neither analysis revealed any evidence to suggest the presence of an Iridovirus in healthy or CCD colonies. PMID:21738798

Fully depleted back illuminated CCD

DOEpatents

Holland, Stephen Edward

2001-01-01

A backside illuminated charge coupled device (CCD) is formed of a relatively thick high resistivity photon sensitive silicon substrate, with frontside electronic circuitry, and an optically transparent backside ohmic contact for applying a backside voltage which is at least sufficient to substantially fully deplete the substrate. A greater bias voltage which overdepletes the substrate may also be applied. One way of applying the bias voltage to the substrate is by physically connecting the voltage source to the ohmic contact. An alternate way of applying the bias voltage to the substrate is to physically connect the voltage source to the frontside of the substrate, at a point outside the depletion region. Thus both frontside and backside contacts can be used for backside biasing to fully deplete the substrate. Also, high resistivity gaps around the CCD channels and electrically floating channel stop regions can be provided in the CCD array around the CCD channels. The CCD array forms an imaging sensor useful in astronomy.

High-Voltage Clock Driver for Photon-Counting CCD Characterization

NASA Technical Reports Server (NTRS)

Baker, Robert

2013-01-01

A document discusses the CCD97 from e2v technologies as it is being evaluated at Goddard Space Flight Center's Detector Characterization Laboratory (DCL) for possible use in ultra-low background noise space astronomy applications, such as Terrestrial Planet Finder Coronagraph (TPF-C). The CCD97 includes a photoncounting mode where the equivalent output noise is less than one electron. Use of this mode requires a clock signal at a voltage level greater than the level achievable by the existing CCD (charge-coupled-device) electronics. A high-voltage waveform generator has been developed in code 660/601 to support the CCD97 evaluation. The unit generates required clock waveforms at voltage levels from -20 to +50 V. It deals with standard and arbitrary waveforms and supports pixel rates from 50 to 500 kHz. The system is designed to interface with existing Leach CCD electronics.

Electronic neural network for dynamic resource allocation

NASA Technical Reports Server (NTRS)

Thakoor, A. P.; Eberhardt, S. P.; Daud, T.

1991-01-01

A VLSI implementable neural network architecture for dynamic assignment is presented. The resource allocation problems involve assigning members of one set (e.g. resources) to those of another (e.g. consumers) such that the global 'cost' of the associations is minimized. The network consists of a matrix of sigmoidal processing elements (neurons), where the rows of the matrix represent resources and columns represent consumers. Unlike previous neural implementations, however, association costs are applied directly to the neurons, reducing connectivity of the network to VLSI-compatible 0 (number of neurons). Each row (and column) has an additional neuron associated with it to independently oversee activations of all the neurons in each row (and each column), providing a programmable 'k-winner-take-all' function. This function simultaneously enforces blocking (excitatory/inhibitory) constraints during convergence to control the number of active elements in each row and column within desired boundary conditions. Simulations show that the network, when implemented in fully parallel VLSI hardware, offers optimal (or near-optimal) solutions within only a fraction of a millisecond, for problems up to 128 resources and 128 consumers, orders of magnitude faster than conventional computing or heuristic search methods.

A parallelizable real-time motion tracking algorithm with applications to ultrasonic strain imaging

NASA Astrophysics Data System (ADS)

Jiang, J.; Hall, T. J.

2007-07-01

Ultrasound-based mechanical strain imaging systems utilize signals from conventional diagnostic ultrasound systems to image tissue elasticity contrast that provides new diagnostically valuable information. Previous works (Hall et al 2003 Ultrasound Med. Biol. 29 427, Zhu and Hall 2002 Ultrason. Imaging 24 161) demonstrated that uniaxial deformation with minimal elevation motion is preferred for breast strain imaging and real-time strain image feedback to operators is important to accomplish this goal. The work reported here enhances the real-time speckle tracking algorithm with two significant modifications. One fundamental change is that the proposed algorithm is a column-based algorithm (a column is defined by a line of data parallel to the ultrasound beam direction, i.e. an A-line), as opposed to a row-based algorithm (a row is defined by a line of data perpendicular to the ultrasound beam direction). Then, displacement estimates from its adjacent columns provide good guidance for motion tracking in a significantly reduced search region to reduce computational cost. Consequently, the process of displacement estimation can be naturally split into at least two separated tasks, computed in parallel, propagating outward from the center of the region of interest (ROI). The proposed algorithm has been implemented and optimized in a Windows® system as a stand-alone ANSI C++ program. Results of preliminary tests, using numerical and tissue-mimicking phantoms, and in vivo tissue data, suggest that high contrast strain images can be consistently obtained with frame rates (10 frames s-1) that exceed our previous methods.

Pathogen Webs in Collapsing Honey Bee Colonies

PubMed Central

Cornman, R. Scott; Tarpy, David R.; Chen, Yanping; Jeffreys, Lacey; Lopez, Dawn; Pettis, Jeffery S.; vanEngelsdorp, Dennis; Evans, Jay D.

2012-01-01

Recent losses in honey bee colonies are unusual in their severity, geographical distribution, and, in some cases, failure to present recognized characteristics of known disease. Domesticated honey bees face numerous pests and pathogens, tempting hypotheses that colony collapses arise from exposure to new or resurgent pathogens. Here we explore the incidence and abundance of currently known honey bee pathogens in colonies suffering from Colony Collapse Disorder (CCD), otherwise weak colonies, and strong colonies from across the United States. Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies. Pathogen loads were highly covariant in CCD but not control hives, suggesting that CCD colonies rapidly become susceptible to a diverse set of pathogens, or that co-infections can act synergistically to produce the rapid depletion of workers that characterizes the disorder. We also tested workers from a CCD-free apiary to confirm that significant positive correlations among pathogen loads can develop at the level of individual bees and not merely as a secondary effect of CCD. This observation and other recent data highlight pathogen interactions as important components of bee disease. Finally, we used deep RNA sequencing to further characterize microbial diversity in CCD and non-CCD hives. We identified novel strains of the recently described Lake Sinai viruses (LSV) and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD. The results are discussed with respect to host-parasite interactions and other environmental stressors of honey bees. PMID:22927991

Comparison of Eight Years Total Column Ozone Retrievals form Brewer and Dobson Spectrophotometers in South Pole

NASA Astrophysics Data System (ADS)

Feng, K. H.; Moeini, O.; McElroy, C. T.; Evans, R. D.; Petropavlovskikh, I. V.

2015-12-01

Total column ozone measured by a Brewer Mark III spectrophotometer (#85) from 2008 to 2015 is compared to the data obtained from three different Dobson spectrophotometers (#80, #82 and #42) that have been operating in parallel with the Brewer at the Amundsen-Scott Station near the South Pole. Measurements are made using either direct sunlight or light from the moon (up to 2 weeks per month). The result of the comparison was used to assess the performance of the two instrument types and determine the stability of the measurement systems. Both instruments suffer from non-linearity due to the presence of instrumental stray light caused by the out-off-band radiations scattered from the optics within the instrument. Stray light results in an underestimated ozone column at large ozone path lengths. Since measurements made at the location of the station (Latitude 89.99o, Longitude -24.80o) have solar zenith angles of 66.5 degrees or greater, the issue of stray light is a particular concern.

The future scientific CCD

NASA Technical Reports Server (NTRS)

Janesick, J. R.; Elliott, T.; Collins, S.; Marsh, H.; Blouke, M. M.

1984-01-01

Since the first introduction of charge-coupled devices (CCDs) in 1970, CCDs have been considered for applications related to memories, logic circuits, and the detection of visible radiation. It is pointed out, however, that the mass market orientation of CCD development has left largely untapped the enormous potential of these devices for advanced scientific instrumentation. The present paper has, therefore, the objective to introduce the CCD characteristics to the scientific community, taking into account prospects for further improvement. Attention is given to evaluation criteria, a summary of current CCDs, CCD performance characteristics, absolute calibration tools, quantum efficiency, aspects of charge collection, charge transfer efficiency, read noise, and predictions regarding the characteristics of the next generation of silicon scientific CCD imagers.

Enhanced performance CCD output amplifier

DOEpatents

Dunham, Mark E.; Morley, David W.

1996-01-01

A low-noise FET amplifier is connected to amplify output charge from a che coupled device (CCD). The FET has its gate connected to the CCD in common source configuration for receiving the output charge signal from the CCD and output an intermediate signal at a drain of the FET. An intermediate amplifier is connected to the drain of the FET for receiving the intermediate signal and outputting a low-noise signal functionally related to the output charge signal from the CCD. The amplifier is preferably connected as a virtual ground to the FET drain. The inherent shunt capacitance of the FET is selected to be at least equal to the sum of the remaining capacitances.

Advances in CCD detector technology for x-ray diffraction applications

NASA Astrophysics Data System (ADS)

Thorson, Timothy A.; Durst, Roger D.; Frankel, Dan; Bordwell, Rex L.; Camara, Jose R.; Leon-Guerrero, Edward; Onishi, Steven K.; Pang, Francis; Vu, Paul; Westbrook, Edwin M.

2004-01-01

Phosphor-coupled CCDs are established as one of the most successful technologies for x-ray diffraction. This application demands that the CCD simultaneously achieve both the highest possible sensitivity and high readout speeds. Recently, wafer-scale, back illuminated devices have become available which offer significantly higher quantum efficiency than conventional devices (the Fairchild Imaging CCD 486 BI). However, since back thinning significantly changes the electrical properties of the CCD the high speed operation of wafer-scale, back-illuminated devices is not well understood. Here we describe the operating characteristics (including noise, linearity, full well capacity and CTE) of the back-illuminated CCD 486 at readout speeds up to 4 MHz.

Scientific CCD technology at JPL

NASA Technical Reports Server (NTRS)

Janesick, J.; Collins, S. A.; Fossum, E. R.

1991-01-01

Charge-coupled devices (CCD's) were recognized for their potential as an imaging technology almost immediately following their conception in 1970. Twenty years later, they are firmly established as the technology of choice for visible imaging. While consumer applications of CCD's, especially the emerging home video camera market, dominated manufacturing activity, the scientific market for CCD imagers has become significant. Activity of the Jet Propulsion Laboratory and its industrial partners in the area of CCD imagers for space scientific instruments is described. Requirements for scientific imagers are significantly different from those needed for home video cameras, and are described. An imager for an instrument on the CRAF/Cassini mission is described in detail to highlight achieved levels of performance.

Colony Collapse Disorder: A Descriptive Study

PubMed Central

vanEngelsdorp, Dennis; Evans, Jay D.; Saegerman, Claude; Mullin, Chris; Haubruge, Eric; Nguyen, Bach Kim; Frazier, Maryann; Frazier, Jim; Cox-Foster, Diana; Chen, Yanping; Underwood, Robyn; Tarpy, David R.; Pettis, Jeffery S.

2009-01-01

Background Over the last two winters, there have been large-scale, unexplained losses of managed honey bee (Apis mellifera L.) colonies in the United States. In the absence of a known cause, this syndrome was named Colony Collapse Disorder (CCD) because the main trait was a rapid loss of adult worker bees. We initiated a descriptive epizootiological study in order to better characterize CCD and compare risk factor exposure between populations afflicted by and not afflicted by CCD. Methods and Principal Findings Of 61 quantified variables (including adult bee physiology, pathogen loads, and pesticide levels), no single measure emerged as a most-likely cause of CCD. Bees in CCD colonies had higher pathogen loads and were co-infected with a greater number of pathogens than control populations, suggesting either an increased exposure to pathogens or a reduced resistance of bees toward pathogens. Levels of the synthetic acaricide coumaphos (used by beekeepers to control the parasitic mite Varroa destructor) were higher in control colonies than CCD-affected colonies. Conclusions/Significance This is the first comprehensive survey of CCD-affected bee populations that suggests CCD involves an interaction between pathogens and other stress factors. We present evidence that this condition is contagious or the result of exposure to a common risk factor. Potentially important areas for future hypothesis-driven research, including the possible legacy effect of mite parasitism and the role of honey bee resistance to pesticides, are highlighted. PMID:19649264

Colony collapse disorder: a descriptive study.

PubMed

Vanengelsdorp, Dennis; Evans, Jay D; Saegerman, Claude; Mullin, Chris; Haubruge, Eric; Nguyen, Bach Kim; Frazier, Maryann; Frazier, Jim; Cox-Foster, Diana; Chen, Yanping; Underwood, Robyn; Tarpy, David R; Pettis, Jeffery S

2009-08-03

Over the last two winters, there have been large-scale, unexplained losses of managed honey bee (Apis mellifera L.) colonies in the United States. In the absence of a known cause, this syndrome was named Colony Collapse Disorder (CCD) because the main trait was a rapid loss of adult worker bees. We initiated a descriptive epizootiological study in order to better characterize CCD and compare risk factor exposure between populations afflicted by and not afflicted by CCD. Of 61 quantified variables (including adult bee physiology, pathogen loads, and pesticide levels), no single measure emerged as a most-likely cause of CCD. Bees in CCD colonies had higher pathogen loads and were co-infected with a greater number of pathogens than control populations, suggesting either an increased exposure to pathogens or a reduced resistance of bees toward pathogens. Levels of the synthetic acaricide coumaphos (used by beekeepers to control the parasitic mite Varroa destructor) were higher in control colonies than CCD-affected colonies. This is the first comprehensive survey of CCD-affected bee populations that suggests CCD involves an interaction between pathogens and other stress factors. We present evidence that this condition is contagious or the result of exposure to a common risk factor. Potentially important areas for future hypothesis-driven research, including the possible legacy effect of mite parasitism and the role of honey bee resistance to pesticides, are highlighted.

Cryostat and CCD for MEGARA at GTC

NASA Astrophysics Data System (ADS)

Castillo-Domínguez, E.; Ferrusca, D.; Tulloch, S.; Velázquez, M.; Carrasco, E.; Gallego, J.; Gil de Paz, A.; Sánchez, F. M.; Vílchez Medina, J. M.

2012-09-01

MEGARA (Multi-Espectrógrafo en GTC de Alta Resolución para Astronomía) is the new integral field unit (IFU) and multi-object spectrograph (MOS) instrument for the GTC. The spectrograph subsystems include the pseudo-slit, the shutter, the collimator with a focusing mechanism, pupil elements on a volume phase holographic grating (VPH) wheel and the camera joined to the cryostat through the last lens, with a CCD detector inside. In this paper we describe the full preliminary design of the cryostat which will harbor the CCD detector for the spectrograph. The selected cryogenic device is an LN2 open-cycle cryostat which has been designed by the "Astronomical Instrumentation Lab for Millimeter Wavelengths" at INAOE. A complete description of the cryostat main body and CCD head is presented as well as all the vacuum and temperature sub-systems to operate it. The CCD is surrounded by a radiation shield to improve its performance and is placed in a custom made mechanical mounting which will allow physical adjustments for alignment with the spectrograph camera. The 4k x 4k pixel CCD231 is our selection for the cryogenically cooled detector of MEGARA. The characteristics of this CCD, the internal cryostat cabling and CCD controller hardware are discussed. Finally, static structural finite element modeling and thermal analysis results are shown to validate the cryostat model.

Robotic CCD microscope for enhanced crystal recognition

DOEpatents

Segelke, Brent W.; Toppani, Dominique

2007-11-06

A robotic CCD microscope and procedures to automate crystal recognition. The robotic CCD microscope and procedures enables more accurate crystal recognition, leading to fewer false negative and fewer false positives, and enable detection of smaller crystals compared to other methods available today.

Optical CT scanning of PRESAGETM polyurethane samples with a CCD-based readout system

NASA Astrophysics Data System (ADS)

Doran, S. J.; Krstajic, N.; Adamovics, J.; Jenneson, P. M.

2004-01-01

This article demonstrates the resolution capabilities of the CCD scanner under ideal circumstances and describes the first CCD-based optical CT experiments on a new class of dosimeter, known as PRESAGETM (Heuris Pharma, Skillman, NJ).

A microfluidic chip with a staircase pH gradient generator, a packed column and a fraction collector for chromatofocusing of proteins.

PubMed

Rho, Hoon Suk; Hanke, Alexander Thomas; Ottens, Marcel; Gardeniers, Han J G E

2018-04-01

A microfluidic device for pH gradient chromatofocusing is presented, which performs creation of a micro-column, pH gradient generation, and fraction collection in a single device. Using a sieve micro-valve, anion exchange particles were packed into a microchannel in order to realize a solid-phase absorption column. To fractionate proteins according to their isoelectric points, elution buffer solutions with a stepwise pH gradient were prepared in 16 parallel mixing reactors and flowed through the micro-column, wherein a protein mixture was previously loaded. The volume of the column is only 20 nL, hence it allows extremely low sample consumption and fast analysis compared with a conventional system. We demonstrated separation of two proteins, albumin-fluorescein isothiocyanate conjugate (FITC-BSA) and R-Phycoerythrin (R-PE), by using a microcolumn of commercial charged polymeric particles (Source 15Q). The microfluidic device can be used as a rapid diagnostic tool to analyse crude mixtures of proteins or nucleic acids and determine adsorption/desorption characteristics of various biochemical products, which can be helpful for scientific fundamental understanding as well as instrumental in various industrial applications, especially in early stage screening and process development. © 2018 The Authors Electrophoresis Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

On-column reduction of catecholamine quinones in stainless steel columns during liquid chromatography.

PubMed

Xu, R; Huang, X; Kramer, K J; Hawley, M D

1995-10-10

The chromatographic behavior of quinones derived from the oxidation of dopamine and N-acetyldopamine has been studied using liquid chromatography (LC) with both a diode array detector and an electrochemical detector that has parallel dual working electrodes. When stainless steel columns are used, an anodic peak for the oxidation of the catecholamine is observed at the same retention time as a cathodic peak for the reduction of the catecholamine quinone. In addition, the anodic peak exhibits a tail that extends to a second anodic peak for the catecholamine. The latter peak occurs at the normal retention time of the catecholamine. The origin of this phenomenon has been studied and metallic iron in the stainless steel components of the LC system has been found to reduce the quinones to their corresponding catecholamines. The simultaneous appearance of a cathodic peak for the reduction of catecholamine quinone and an anodic peak for the oxidation of the corresponding catecholamine occurs when metallic iron in the exit frit reduces some of the quinones as the latter exits the column. This phenomenon is designated as the "concurrent anodic-cathodic response." It is also observed for quinones of of 3,4-dihydroxybenzoic acid and probably occurs with o- or p-quinones of other dihydroxyphenyl compounds. The use of nonferrous components in LC systems is recommended to eliminate possible on-column reduction of quinones.

Supramolecular hydrogen-bonding networks in bis(adeninium) phthalate phthalic acid 1.45-hydrate.

PubMed

Sridhar, Balasubramanian; Ravikumar, Krishnan

2007-04-01

In the title compound, 2C(5)H(6)N(5)(+).C(8)H(4)O(4)(2-).C(8)H(6)O(4).1.45H(2)O, the asymmetric unit comprises two adeninium cations, two half phthalate anions with crystallographic C(2) symmetry, one neutral phthalic acid molecule, and one fully occupied and one partially occupied site (0.45) for water molecules. The adeninium cations form N-H...O hydrogen bonds with the phthalate anions. The cations also form infinite one-dimensional polymeric ribbons via N-H...N interactions. In the crystal packing, hydrogen-bonded columns of cations, anions and phthalate anions extend parallel to the c axis. The water molecules crosslink adjacent columns into hydrogen-bonded layers.

Spectrum of Celiac disease in Paediatric population: Experience of Tertiary Care Center from Pakistan.

PubMed

Aziz, Danish Abdul; Kahlid, Misha; Memon, Fozia; Sadiq, Kamran

2017-01-01

To determine clinical features and relevant laboratory investigations of patient with celiac disease (CD) and comparing classical celiac disease (CCD) with Non-diarrheal celiac disease (NDCD). This is a five years retrospective study conducted at The Aga Khan University Hospital Karachi, Pakistan from January 2010 to December 2015, enrolling children from one year to 15 years of either gender diagnosed as celiac disease in accordance with revised ESPGHAN criteria. Biopsy samples with grade 2 or more on Modified Marsh Classification were considered as consistent with celiac disease. Celiac patients were categorized into Classical celiac disease (with Chronic Diarrhea) and non-diarrheal celiac disease (Atypical celiac) and their clinical features and relevant laboratory investigations were documented. Total 66 patients were selected with celiac disease according to inclusion criteria, 39 (59.09%) patients were labeled as CCD and 27 (40.91%) patients were labeled as NDCD. Marsh grading 3a and above were more marked in CCD as compared to NDCD. Mean titer for Tissue transglutaminase antibodies (TTG) were higher in CCD group in comparison to NDCD group. In CCD, the most common clinical presentations were abdominal distension whereas in NDCD, the most remarkable features were recurrent abdominal pain (62.9%). Frequency of failure to thrive is significantly high in CCD (82.05%) but patients merely with short stature were more common in NDCD (33.3%). Refractory anemia was present in 66.6% patients in NDCD group and 41.1% patients in CCD group. 74.3% patients in CCD group were vitamin D deficient whereas 85% patient had vitamin D deficiency in NDCD group (p= 0.03). NDCD is not uncommon in our population. Recurrent abdominal pain, failure to thrive or patients only with short stature and refractory anemia are prominent features in NCDC group whereas abdominal distension, failure to thrive and recurrent abdominal pain were noticeable features in CCD. High grade histopathology and raised antibodies titer is hallmark of CCD. Vitamin D deficiency is almost equally present in both groups.

CCD filter and transform techniques for interference excision

NASA Technical Reports Server (NTRS)

Borsuk, G. M.; Dewitt, R. N.

1976-01-01

The theoretical and some experimental results of a study aimed at applying CCD filter and transform techniques to the problem of interference excision within communications channels were presented. Adaptive noise (interference) suppression was achieved by the modification of received signals such that they were orthogonal to the recently measured noise field. CCD techniques were examined to develop real-time noise excision processing. They were recursive filters, circulating filter banks, transversal filter banks, an optical implementation of the chirp Z transform, and a CCD analog FFT.

VizieR Online Data Catalog: BVR light curves of UZ Leo (Lee+, 2018)

NASA Astrophysics Data System (ADS)

Lee, J. W.; Park, J.-H.

2018-04-01

We performed new CCD photometry of UZ Leo during two observing seasons between 2012 February and 2013 April, using a PIXIS: 2048B CCD and a BVR filter set attached to the 61 cm reflector at Sobaeksan Optical Astronomy Observatory (SOAO) in Korea. The CCD chip has 2048x2048pixels and a pixel size of 13.5um, so the field of view of a CCD frame is 17.6'x17.6'. (1 data file).

Colony Collapse Disorder (CCD) and bee age impact honey bee pathophysiology

PubMed Central

Traynor, Kirsten S.; Andree, Michael; Lichtenberg, Elinor M.; Chen, Yanping; Saegerman, Claude; Cox-Foster, Diana L.

2017-01-01

Honey bee (Apis mellifera) colonies continue to experience high annual losses that remain poorly explained. Numerous interacting factors have been linked to colony declines. Understanding the pathways linking pathophysiology with symptoms is an important step in understanding the mechanisms of disease. In this study we examined the specific pathologies associated with honey bees collected from colonies suffering from Colony Collapse Disorder (CCD) and compared these with bees collected from apparently healthy colonies. We identified a set of pathological physical characteristics that occurred at different rates in CCD diagnosed colonies prior to their collapse: rectum distension, Malpighian tubule iridescence, fecal matter consistency, rectal enteroliths (hard concretions), and venom sac color. The multiple differences in rectum symptomology in bees from CCD apiaries and colonies suggest effected bees had trouble regulating water. To ensure that pathologies we found associated with CCD were indeed pathologies and not due to normal changes in physical appearances that occur as an adult bee ages (CCD colonies are assumed to be composed mostly of young bees), we documented the changes in bees of different ages taken from healthy colonies. We found that young bees had much greater incidences of white nodules than older cohorts. Prevalent in newly-emerged bees, these white nodules or cellular encapsulations indicate an active immune response. Comparing the two sets of characteristics, we determined a subset of pathologies that reliably predict CCD status rather than bee age (fecal matter consistency, rectal distension size, rectal enteroliths and Malpighian tubule iridescence) and that may serve as biomarkers for colony health. In addition, these pathologies suggest that CCD bees are experiencing disrupted excretory physiology. Our identification of these symptoms is an important first step in understanding the physiological pathways that underlie CCD and factors impacting bee health. PMID:28715431

Colony Collapse Disorder (CCD) and bee age impact honey bee pathophysiology.

PubMed

vanEngelsdorp, Dennis; Traynor, Kirsten S; Andree, Michael; Lichtenberg, Elinor M; Chen, Yanping; Saegerman, Claude; Cox-Foster, Diana L

2017-01-01

Honey bee (Apis mellifera) colonies continue to experience high annual losses that remain poorly explained. Numerous interacting factors have been linked to colony declines. Understanding the pathways linking pathophysiology with symptoms is an important step in understanding the mechanisms of disease. In this study we examined the specific pathologies associated with honey bees collected from colonies suffering from Colony Collapse Disorder (CCD) and compared these with bees collected from apparently healthy colonies. We identified a set of pathological physical characteristics that occurred at different rates in CCD diagnosed colonies prior to their collapse: rectum distension, Malpighian tubule iridescence, fecal matter consistency, rectal enteroliths (hard concretions), and venom sac color. The multiple differences in rectum symptomology in bees from CCD apiaries and colonies suggest effected bees had trouble regulating water. To ensure that pathologies we found associated with CCD were indeed pathologies and not due to normal changes in physical appearances that occur as an adult bee ages (CCD colonies are assumed to be composed mostly of young bees), we documented the changes in bees of different ages taken from healthy colonies. We found that young bees had much greater incidences of white nodules than older cohorts. Prevalent in newly-emerged bees, these white nodules or cellular encapsulations indicate an active immune response. Comparing the two sets of characteristics, we determined a subset of pathologies that reliably predict CCD status rather than bee age (fecal matter consistency, rectal distension size, rectal enteroliths and Malpighian tubule iridescence) and that may serve as biomarkers for colony health. In addition, these pathologies suggest that CCD bees are experiencing disrupted excretory physiology. Our identification of these symptoms is an important first step in understanding the physiological pathways that underlie CCD and factors impacting bee health.

Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry.

PubMed

Gu, Qun; David, Frank; Lynen, Frédéric; Rumpel, Klaus; Xu, Guowang; De Vos, Paul; Sandra, Pat

2010-06-25

Comprehensive two-dimensional gas chromatography (GCxGC) offers an interesting tool for profiling bacterial fatty acids. Flow modulated GCxGC using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. The method was tested on bacterial fatty acid methyl esters (BAMEs) from Stenotrophomonas maltophilia LMG 958T by using parallel flame ionization detector (FID)/mass spectrometry (MS). The results are compared to data obtained using a thermal modulated GCxGC system. The data show that flow modulated GCxGC-FID/MS method can be applied in a routine environment and offers interesting perspectives for chemotaxonomy of bacteria.

Actinide chemistry using singlet-paired coupled cluster and its combinations with density functionals

NASA Astrophysics Data System (ADS)

Garza, Alejandro J.; Sousa Alencar, Ana G.; Scuseria, Gustavo E.

2015-12-01

Singlet-paired coupled cluster doubles (CCD0) is a simplification of CCD that relinquishes a fraction of dynamic correlation in order to be able to describe static correlation. Combinations of CCD0 with density functionals that recover specifically the dynamic correlation missing in the former have also been developed recently. Here, we assess the accuracy of CCD0 and CCD0+DFT (and variants of these using Brueckner orbitals) as compared to well-established quantum chemical methods for describing ground-state properties of singlet actinide molecules. The f0 actinyl series (UO22+, NpO23+, PuO24+), the isoelectronic NUN, and thorium (ThO, ThO2+) and nobelium (NoO, NoO2) oxides are studied.

MUSIC - Multifunctional stereo imaging camera system for wide angle and high resolution stereo and color observations on the Mars-94 mission

NASA Astrophysics Data System (ADS)

Oertel, D.; Jahn, H.; Sandau, R.; Walter, I.; Driescher, H.

1990-10-01

Objectives of the multifunctional stereo imaging camera (MUSIC) system to be deployed on the Soviet Mars-94 mission are outlined. A high-resolution stereo camera (HRSC) and wide-angle opto-electronic stereo scanner (WAOSS) are combined in terms of hardware, software, technology aspects, and solutions. Both HRSC and WAOSS are push-button instruments containing a single optical system and focal plates with several parallel CCD line sensors. Emphasis is placed on the MUSIC system's stereo capability, its design, mass memory, and data compression. A 1-Gbit memory is divided into two parts: 80 percent for HRSC and 20 percent for WAOSS, while the selected on-line compression strategy is based on macropixel coding and real-time transform coding.

A programmable CCD driver circuit for multiphase CCD operation

NASA Technical Reports Server (NTRS)

Ewin, Audrey J.; Reed, Kenneth V.

1989-01-01

A programmable CCD (charge-coupled device) driver circuit was designed to drive CCDs in multiphased modes. The purpose of the drive electronics is to operate developmental CCD imaging arrays for NASA's tiltable moderate resolution imaging spectrometer (MODIS-T). Five objectives for the driver were considered during its design: (1) the circuit drives CCD electrode voltages between 0 V and +30 V to produce reasonable potential wells, (2) the driving sequence is started with one input signal, (3) the driving sequence is started with one input signal, (4) the circuit allows programming of frame sequences required by arrays of any size, (5) it produces interfacing signals for the CCD and the DTF (detector test facility). Simulation of the driver verified its function with the master clock running up to 10 MHz. This suggests a maximum rate of 400,000 pixels/s. Timing and packaging parameters were verified. The design uses 54 TTL (transistor-transistor logic) chips. Two versions of hardware were fabricated: wirewrap and printed circuit board. Both were verified functionally with a logic analyzer.

Multiple Beam Optical Processing

DTIC Science & Technology

1989-12-01

the interference of multiple reflections between the two mirrors. The most promising optical bistable devices, at present, are very thin, solid Fabry...MEDIUM b) R - Ir ,, PMASE SHIFTr Figure 1.3 (a) Nonlinear Fabry-Perot etalon consisting of solid material with parallel surfaces with coatings of...instead of the solid planar structure [2.10]. Voids between columns cause an Inhomogeneous broadening and an exponential extension (Urbach tail) of the

Airborne Lidar Measurements of Below-canopy Surface Water Height , Slope and Optical Properties in the Florida Everglades Shark River Slough

NASA Astrophysics Data System (ADS)

Dabney, P.; Harding, D. J.; Valett, S. R.; Yu, A. W.; Feliciano, E. A.; Neuenschwander, A. L.; Pitts, K.

2015-12-01

Determining the presence, persistence, optical properties and variation in height and slope of surface water beneath the dense canopies of flooded forests and mangrove stands could contribute to studies of the acquisition of water and nutrients by plant roots. NASA's airborne Slope Imaging Multi-polarization Photon-counting Lidar (SIMPL) provides unique capabilities that can identify below-canopy surface water, measure its height with respect to vegetation constituents with sub-decimeter precision and quantify its slope. It also provides information on canopy structure and closure, the water column extinction profile as a proxy for turbidity and water depth, with the penetration depth constrained by turbidity. It achieves this by using four laser beams operating at two wavelengths with measurements of water surface elevation at 1064 nm (near infrared) and water column properties at 532 nm (green), analogous to a bathymetric lidar. Importantly the instrument adds a polarimetry function, like some atmospheric lidars, which measures the amount of depolarization determined by the degree to which the plane-parallel transmitted laser pulse energy is converted to the perpendicular state. The degree of depolarization is sensitive to the number of photon multiple-scattering events. For the water surface, which is specular consisting only of single-scattering events, the near-infrared received signal retains the parallel polarization state. Absence of the perpendicular signal uniquely identifies surface water. Penetration of green light and the depth profile of photons converted to the perpendicular state compared to those in the parallel state is a measure of water-column multiple scattering, providing a relative measure of turbidity. The amount of photons reflected from the canopy versus the water provides a wavelength-dependent measure of canopy closure. By rapidly firing laser pulses (11,400 pulses per second) with a narrow width (1 nsec) and detecting single photons with 8 cm ranging precision, the surface altimetry data is acquired with very high spatial and vertical resolution. Examples of these capabilities will be shown using data collected in 2011 along and across the flow axis of the Florida Everglades Shark River Slough, targeting the slough's Long Term Ecology Research (LTER) field sites.

A common origin for the bacterial toxin-antitoxin systems parD and ccd, suggested by analyses of toxin/target and toxin/antitoxin interactions.

PubMed

Smith, Andrew B; López-Villarejo, Juan; Diago-Navarro, Elizabeth; Mitchenall, Lesley A; Barendregt, Arjan; Heck, Albert J; Lemonnier, Marc; Maxwell, Anthony; Díaz-Orejas, Ramón

2012-01-01

Bacterial toxin-antitoxin (TA) systems encode two proteins, a potent inhibitor of cell proliferation (toxin) and its specific antidote (antitoxin). Structural data has revealed striking similarities between the two model TA toxins CcdB, a DNA gyrase inhibitor encoded by the ccd system of plasmid F, and Kid, a site-specific endoribonuclease encoded by the parD system of plasmid R1. While a common structural fold seemed at odds with the two clearly different modes of action of these toxins, the possibility of functional crosstalk between the parD and ccd systems, which would further point to their common evolutionary origin, has not been documented. Here, we show that the cleavage of RNA and the inhibition of protein synthesis by the Kid toxin, two activities that are specifically counteracted by its cognate Kis antitoxin, are altered, but not inhibited, by the CcdA antitoxin. In addition, Kis was able to inhibit the stimulation of DNA gyrase-mediated cleavage of DNA by CcdB, albeit less efficiently than CcdA. We further show that physical interactions between the toxins and antitoxins of the different systems do occur and define the stoichiometry of the complexes formed. We found that CcdB did not degrade RNA nor did Kid have any reproducible effect on the tested DNA gyrase activities, suggesting that these toxins evolved to reach different, rather than common, cellular targets.

Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera).

PubMed

Johnson, Reed M; Evans, Jay D; Robinson, Gene E; Berenbaum, May R

2009-09-01

Colony collapse disorder (CCD) is a mysterious disappearance of honey bees that has beset beekeepers in the United States since late 2006. Pathogens and other environmental stresses, including pesticides, have been linked to CCD, but a causal relationship has not yet been demonstrated. Because the gut acts as a primary interface between the honey bee and its environment as a site of entry for pathogens and toxins, we used whole-genome microarrays to compare gene expression between guts of bees from CCD colonies originating on both the east and west coasts of the United States and guts of bees from healthy colonies sampled before the emergence of CCD. Considerable variation in gene expression was associated with the geographical origin of bees, but a consensus list of 65 transcripts was identified as potential markers for CCD status. Overall, elevated expression of pesticide response genes was not observed. Genes involved in immune response showed no clear trend in expression pattern despite the increased prevalence of viruses and other pathogens in CCD colonies. Microarray analysis revealed unusual ribosomal RNA fragments that were conspicuously more abundant in the guts of CCD bees. The presence of these fragments may be a possible consequence of picorna-like viral infection, including deformed wing virus and Israeli acute paralysis virus, and may be related to arrested translation. Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD.

Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)

PubMed Central

Johnson, Reed M.; Evans, Jay D.; Robinson, Gene E.; Berenbaum, May R.

2009-01-01

Colony collapse disorder (CCD) is a mysterious disappearance of honey bees that has beset beekeepers in the United States since late 2006. Pathogens and other environmental stresses, including pesticides, have been linked to CCD, but a causal relationship has not yet been demonstrated. Because the gut acts as a primary interface between the honey bee and its environment as a site of entry for pathogens and toxins, we used whole-genome microarrays to compare gene expression between guts of bees from CCD colonies originating on both the east and west coasts of the United States and guts of bees from healthy colonies sampled before the emergence of CCD. Considerable variation in gene expression was associated with the geographical origin of bees, but a consensus list of 65 transcripts was identified as potential markers for CCD status. Overall, elevated expression of pesticide response genes was not observed. Genes involved in immune response showed no clear trend in expression pattern despite the increased prevalence of viruses and other pathogens in CCD colonies. Microarray analysis revealed unusual ribosomal RNA fragments that were conspicuously more abundant in the guts of CCD bees. The presence of these fragments may be a possible consequence of picorna-like viral infection, including deformed wing virus and Israeli acute paralysis virus, and may be related to arrested translation. Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD. PMID:19706391

Cognitive-communication disorder following right hemisphere stroke: exploring rehabilitation access and outcomes.

PubMed

Hewetson, Ronelle; Cornwell, Petrea; Shum, David

2017-07-01

Rehabilitation positively influences return to activities and social roles in people with aphasia. The cognitive-communication disorder (CCD) found following a right hemisphere stroke has been less extensively researched with rehabilitation access and outcomes yet to be determined. To document rehabilitation access and outcomes for people with CCD post-stroke; and compare outcomes based on presence (viz CCD; aphasia) or absence of communication impairment. A retrospective chart audit was completed for patients with first onset unilateral stroke, with a hospital length of stay (LOS) of at least two days and a communication assessment by a speech pathologist. Data extracted included presence and severity of communication impairment, access to and LOS in a rehabilitation unit, and functional outcome measures recorded at rehabilitation discharge. The majority of the 115 patients who met inclusion criteria were living independently (n = 112, 97.4%) at the time of stroke. CCD (66%) was diagnosed with similar frequency to aphasia (68%). The presence of communication impairment did not result in significant differences in rehabilitation LOS and discharge destination when compared to hemispheric strokes without communication impairment. Severity of CCD was an independent predictor of functional gain by rehabilitation discharge. People with CCD require comparable access to rehabilitation as people with aphasia, and severity of CCD should be considered in determining rehabilitation LOS. A large number of people are discharged with ongoing CCD which warrants exploration of potential participation restrictions created by the communication impairment.

A Common Origin for the Bacterial Toxin-Antitoxin Systems parD and ccd, Suggested by Analyses of Toxin/Target and Toxin/Antitoxin Interactions

PubMed Central

Mitchenall, Lesley A.; Barendregt, Arjan; Heck, Albert J.; Lemonnier, Marc; Maxwell, Anthony; Díaz-Orejas, Ramón

2012-01-01

Bacterial toxin-antitoxin (TA) systems encode two proteins, a potent inhibitor of cell proliferation (toxin) and its specific antidote (antitoxin). Structural data has revealed striking similarities between the two model TA toxins CcdB, a DNA gyrase inhibitor encoded by the ccd system of plasmid F, and Kid, a site-specific endoribonuclease encoded by the parD system of plasmid R1. While a common structural fold seemed at odds with the two clearly different modes of action of these toxins, the possibility of functional crosstalk between the parD and ccd systems, which would further point to their common evolutionary origin, has not been documented. Here, we show that the cleavage of RNA and the inhibition of protein synthesis by the Kid toxin, two activities that are specifically counteracted by its cognate Kis antitoxin, are altered, but not inhibited, by the CcdA antitoxin. In addition, Kis was able to inhibit the stimulation of DNA gyrase-mediated cleavage of DNA by CcdB, albeit less efficiently than CcdA. We further show that physical interactions between the toxins and antitoxins of the different systems do occur and define the stoichiometry of the complexes formed. We found that CcdB did not degrade RNA nor did Kid have any reproducible effect on the tested DNA gyrase activities, suggesting that these toxins evolved to reach different, rather than common, cellular targets. PMID:23029540

Remediation of RDX- and HMX-contaminated groundwater using organic mulch permeable reactive barriers.

PubMed

Ahmad, Farrukh; Schnitker, Stephen P; Newell, Charles J

2007-02-20

Organic mulch is a complex organic material that is typically populated with its own consortium of microorganisms. The organisms in mulch breakdown complex organics to soluble carbon, which can then be used by these and other microorganisms as an electron donor for treating RDX and HMX via reductive pathways. A bench-scale treatability study with organic mulch was conducted for the treatment of RDX- and HMX-contaminated groundwater obtained from a plume at the Pueblo Chemical Depot (PCD) in Pueblo, Colorado. The site-specific cleanup criteria of 0.55 ppb RDX and 602 ppb HMX were used as the logical goals of the study. Column flow-through tests were run to steady-state at the average site seepage velocity, using a 70%:30% (vol.:vol.) mulch:pea gravel packing to approach the formation's permeability. Significant results included: (1) Complete removal of 90 ppb influent RDX and 8 ppb influent HMX in steady-state mulch column effluent; (2) pseudo-first-order steady-state kinetic rate constant, k, of 0.20 to 0.27 h(-1) based on RDX data, using triplicate parallel column runs; (3) accumulation of reduced RDX intermediates in the steady-state column effluent at less than 2% of the influent RDX mass; (4) no binding of RDX to the column fill material; and (5) no leaching of RDX, HMX or reduction intermediates from the column fill material. The results of the bench-scale study will be used to design and implement a pilot-scale organic mulch/pea gravel permeable reactive barrier (PRB) at the site.

Bi-sensory, striped representations: comparative insights from owl and platypus.

PubMed

Pettigrew, John D

2004-01-01

Bi-sensory striped arrays are described in owl and platypus that share some similarities with the other variant of bi-sensory striped array found in primate and carnivore striate cortex: ocular dominance columns. Like ocular dominance columns, the owl and platypus striped systems each involve two different topographic arrays that are cut into parallel stripes, and interdigitated, so that higher-order neurons can integrate across both arrays. Unlike ocular dominance stripes, which have a separate array for each eye, the striped array in the middle third of the owl tectum has a separate array for each cerebral hemisphere. Binocular neurons send outputs from both hemispheres to the striped array where they are segregated into parallel stripes according to hemisphere of origin. In platypus primary somatosensory cortex (S1), the two arrays of interdigitated stripes are derived from separate sensory systems in the bill, 40,000 electroreceptors and 60,000 mechanoreceptors. The stripes in platypus S1 cortex produce bimodal electrosensory-mechanosensory neurons with specificity for the time-of-arrival difference between the two systems. This "thunder-and-lightning" system would allow the platypus to estimate the distance of the prey using time disparities generated at the bill between the earlier electrical wave and the later mechanical wave caused by the motion of benthic prey. The functional significance of parallel, striped arrays is not clear, even for the highly-studied ocular dominance system, but a general strategy is proposed here that is based on the detection of temporal disparities between the two arrays that can be used to estimate distance.

New x-ray parallel beam facility XPBF 2.0 for the characterization of silicon pore optics

NASA Astrophysics Data System (ADS)

Krumrey, Michael; Müller, Peter; Cibik, Levent; Collon, Max; Barrière, Nicolas; Vacanti, Giuseppe; Bavdaz, Marcos; Wille, Eric

2016-07-01

A new X-ray parallel beam facility (XPBF 2.0) has been installed in the laboratory of the Physikalisch-Technische Bundesanstalt at the synchrotron radiation facility BESSY II in Berlin to characterize silicon pore optics (SPOs) for the future X-ray observatory ATHENA. As the existing XPBF which is operated since 2005, the new beamline provides a pencil beam of very low divergence, a vacuum chamber with a hexapod system for accurate positioning of the SPO to be investigated, and a vertically movable CCD-based camera system to register the direct and the reflected beam. In contrast to the existing beamline, a multilayer-coated toroidal mirror is used for beam monochromatization at 1.6 keV and collimation, enabling the use of beam sizes between about 100 μm and at least 5 mm. Thus the quality of individual pores as well as the focusing properties of large groups of pores can be investigated. The new beamline also features increased travel ranges for the hexapod to cope with larger SPOs and a sample to detector distance of 12 m corresponding to the envisaged focal length of ATHENA.

CCD Detects Two Images In Quick Succession

NASA Technical Reports Server (NTRS)

Janesick, James R.; Collins, Andy

1996-01-01

Prototype special-purpose charge-coupled device (CCD) designed to detect two 1,024 x 1,024-pixel images in rapid succession. Readout performed slowly to minimize noise. CCD operated in synchronism with pulsed laser, stroboscope, or other pulsed source of light to form pairs of images of rapidly moving objects.

IAPV, a bee-affecting virus associated with Colony Collapse Disorder can be silenced by dsRNA ingestion.

PubMed

Maori, E; Paldi, N; Shafir, S; Kalev, H; Tsur, E; Glick, E; Sela, I

2009-02-01

Colony Collapse Disorder (CCD) has been associated with Israeli acute paralysis virus (IAPV). CCD poses a serious threat to apiculture and agriculture as a whole, due to the consequent inability to provide the necessary amount of bees for pollination of critical crops. Here we report on RNAi-silencing of IAPV infection by feeding bees with double-stranded RNA, as an efficient and feasible way of controlling this viral disease. The association of CCD with IAPV is discussed, as well as the potential of controlling CCD.

SCP -- A Simple CCD Processing Package

NASA Astrophysics Data System (ADS)

Lewis, J. R.

This note describes a small set of programs, written at RGO, which deal with basic CCD frame processing (e.g. bias subtraction, flat fielding, trimming etc.). The need to process large numbers of CCD frames from devices such as FOS or ISIS in order to extract spectra has prompted the writing of routines which will do the basic hack-work with a minimal amount of interaction from the user. Although they were written with spectral data in mind, there are no ``spectrum-specific'' features in the software which means they can be applied to any CCD data.

Software design of control system of CCD side-scatter lidar

NASA Astrophysics Data System (ADS)

Kuang, Zhiqiang; Liu, Dong; Deng, Qian; Zhang, Zhanye; Wang, Zhenzhu; Yu, Siqi; Tao, Zongming; Xie, Chenbo; Wang, Yingjian

2018-03-01

Because of the existence of blind zone and transition zone, the application of backscattering lidar in near-ground is limited. The side-scatter lidar equipped with the Charge Coupled Devices (CCD) can separate the transmitting and receiving devices to avoid the impact of the geometric factors which is exited in the backscattering lidar and, detect the more precise near-ground aerosol signals continuously. Theories of CCD side-scatter lidar and the design of control system are introduced. The visible control of laser and CCD and automatic data processing method of the side-scatter lidar are developed by using the software of Visual C #. The results which are compared with the calibration of the atmospheric aerosol lidar data show that signals from the CCD side- scatter lidar are convincible.

Regulation of transport in the connecting tubule and cortical collecting duct

PubMed Central

Staruschenko, Alexander

2012-01-01

The central goal of this overview article is to summarize recent findings in renal epithelial transport, focusing chiefly on the connecting tubule (CNT) and the cortical collecting duct (CCD). Mammalian CCD and CNT are involved in fine tuning of electrolyte and fluid balance through reabsorption and secretion. Specific transporters and channels mediate vectorial movements of water and solutes in these segments. Although only a small percent of the glomerular filtrate reaches the CNT and CCD, these segments are critical for water and electrolyte homeostasis since several hormones, e.g. aldosterone and arginine vasopressin, exert their main effects in these nephron sites. Importantly, hormones regulate the function of the entire nephron and kidney by affecting channels and transporters in the CNT and CCD. Knowledge about the physiological and pathophysiological regulation of transport in the CNT and CCD and particular roles of specific channels/transporters has increased tremendously over the last two decades. Recent studies shed new light on several key questions concerning the regulation of renal transport. Precise distribution patterns of transport proteins in the CCD and CNT will be reviewed, and their physiological roles and mechanisms mediating ion transport in these segments will be also covered. Special emphasis will be given to pathophysiological conditions appearing as a result of abnormalities in renal transport in the CNT and CCD. PMID:23227301

Design of area array CCD image acquisition and display system based on FPGA

NASA Astrophysics Data System (ADS)

Li, Lei; Zhang, Ning; Li, Tianting; Pan, Yue; Dai, Yuming

2014-09-01

With the development of science and technology, CCD(Charge-coupled Device) has been widely applied in various fields and plays an important role in the modern sensing system, therefore researching a real-time image acquisition and display plan based on CCD device has great significance. This paper introduces an image data acquisition and display system of area array CCD based on FPGA. Several key technical challenges and problems of the system have also been analyzed and followed solutions put forward .The FPGA works as the core processing unit in the system that controls the integral time sequence .The ICX285AL area array CCD image sensor produced by SONY Corporation has been used in the system. The FPGA works to complete the driver of the area array CCD, then analog front end (AFE) processes the signal of the CCD image, including amplification, filtering, noise elimination, CDS correlation double sampling, etc. AD9945 produced by ADI Corporation to convert analog signal to digital signal. Developed Camera Link high-speed data transmission circuit, and completed the PC-end software design of the image acquisition, and realized the real-time display of images. The result through practical testing indicates that the system in the image acquisition and control is stable and reliable, and the indicators meet the actual project requirements.

Silicon sample holder for molecular beam epitaxy on pre-fabricated integrated circuits

NASA Technical Reports Server (NTRS)

Hoenk, Michael E. (Inventor); Grunthaner, Paula J. (Inventor); Grunthaner, Frank J. (Inventor)

1994-01-01

The sample holder of the invention is formed of the same semiconductor crystal as the integrated circuit on which the molecular beam expitaxial process is to be performed. In the preferred embodiment, the sample holder comprises three stacked micro-machined silicon wafers: a silicon base wafer having a square micro-machined center opening corresponding in size and shape to the active area of a CCD imager chip, a silicon center wafer micro-machined as an annulus having radially inwardly pointing fingers whose ends abut the edges of and center the CCD imager chip within the annulus, and a silicon top wafer micro-machined as an annulus having cantilevered membranes which extend over the top of the CCD imager chip. The micro-machined silicon wafers are stacked in the order given above with the CCD imager chip centered in the center wafer and sandwiched between the base and top wafers. The thickness of the center wafer is about 20% less than the thickness of the CCD imager chip. Preferably, four titanium wires, each grasping the edges of the top and base wafers, compress all three wafers together, flexing the cantilever fingers of the top wafer to accommodate the thickness of the CCD imager chip, acting as a spring holding the CCD imager chip in place.

Overexpression of the rice carotenoid cleavage dioxygenase 1 gene in Golden Rice endosperm suggests apocarotenoids as substrates in planta.

PubMed

Ilg, Andrea; Yu, Qiuju; Schaub, Patrick; Beyer, Peter; Al-Babili, Salim

2010-08-01

Carotenoids are converted by carotenoid cleavage dioxygenases that catalyze oxidative cleavage reactions leading to apocarotenoids. However, apocarotenoids can also be further truncated by some members of this enzyme family. The plant carotenoid cleavage dioxygenase 1 (CCD1) subfamily is known to degrade both carotenoids and apocarotenoids in vitro, leading to different volatile compounds. In this study, we investigated the impact of the rice CCD1 (OsCCD1) on the pigmentation of Golden Rice 2 (GR2), a genetically modified rice variety accumulating carotenoids in the endosperm. For this purpose, the corresponding cDNA was introduced into the rice genome under the control of an endosperm-specific promoter in sense and anti-sense orientations. Despite high expression levels of OsCCD1 in sense plants, pigment analysis revealed carotenoid levels and patterns comparable to those of GR2, pleading against carotenoids as substrates in rice endosperm. In support, similar carotenoid contents were determined in anti-sense plants. To check whether OsCCD1 overexpressed in GR2 endosperm is active, in vitro assays were performed with apocarotenoid substrates. HPLC analysis confirmed the cleavage activity of introduced OsCCD1. Our data indicate that apocarotenoids rather than carotenoids are the substrates of OsCCD1 in planta.

A Chip-Capillary Hybrid Device for Automated Transfer of Sample Pre-Separated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation

PubMed Central

Lu, Joann J.; Wang, Shili; Li, Guanbin; Wang, Wei; Pu, Qiaosheng; Liu, Shaorong

2012-01-01

In this report, we introduce a chip-capillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) or capillary gel electrophoresis (CGE) toward automating two-dimensional (2D) protein separations. The hybrid device consists of three chips that are butted together. The middle chip can be moved between two positions to re-route the fluidic paths, which enables the performance of CIEF and injection of proteins partially resolved by CIEF to CGE capillaries for parallel CGE separations in a continuous and automated fashion. Capillaries are attached to the other two chips to facilitate CIEF and CGE separations and to extend the effective lengths of CGE columns. Specifically, we illustrate the working principle of the hybrid device, develop protocols for producing and preparing the hybrid device, and demonstrate the feasibility of using this hybrid device for automated injection of CIEF-separated sample to parallel CGE for 2D protein separations. Potentials and problems associated with the hybrid device are also discussed. PMID:22830584

New prototype of acousto-optical radio-wave spectrometer with parallel frequency processing for astrophysical applications

NASA Astrophysics Data System (ADS)

Shcherbakov, Alexandre S.; Chavez Dagostino, Miguel; Arellanes, Adan O.; Aguirre Lopez, Arturo

2016-09-01

We develop a multi-band spectrometer with a few spatially parallel optical arms for the combined processing of their data flow. Such multi-band capability has various applications in astrophysical scenarios at different scales: from objects in the distant universe to planetary atmospheres in the Solar system. Each optical arm exhibits original performances to provide parallel multi-band observations with different scales simultaneously. Similar possibility is based on designing each optical arm individually via exploiting different materials for acousto-optical cells operating within various regimes, frequency ranges and light wavelengths from independent light sources. Individual beam shapers provide both the needed incident light polarization and the required apodization to increase the dynamic range of a system. After parallel acousto-optical processing, data flows are united by the joint CCD matrix on the stage of the combined electronic data processing. At the moment, the prototype combines still three bands, i.e. includes three spatial optical arms. The first low-frequency arm operates at the central frequencies 60-80 MHz with frequency bandwidth 40 MHz. The second arm is oriented to middle-frequencies 350-500 MHz with frequency bandwidth 200-300 MHz. The third arm is intended for ultra-high-frequency radio-wave signals about 1.0-1.5 GHz with frequency bandwidth <300 MHz. To-day, this spectrometer has the following preliminary performances. The first arm exhibits frequency resolution 20 KHz; while the second and third arms give the resolution 150-200 KHz. The numbers of resolvable spots are 1500- 2000 depending on the regime of operation. The fourth optical arm at the frequency range 3.5 GHz is currently under construction.

Fabrication of Robust, Flat, Thinned, UV-Imaging CCDs

NASA Technical Reports Server (NTRS)

Grunthaner, Paula; Elliott, Stythe; Jones, Todd; Nikzad, Shouleh

2004-01-01

An improved process that includes a high-temperature bonding subprocess has been developed to enable the fabrication of robust, flat, silicon-based charge-coupled devices (CCDs) for imaging in ultraviolet (UV) light and/or for detecting low-energy charged particles. The CCDs in question are devices on which CCD circuitry has already been formed and have been thinned for backsurface illumination. These CCDs may be delta doped, and aspects of this type of CCD have been described in several prior articles in NASA Tech Briefs. Unlike prior low-temperature bonding subprocesses based on the use of epoxies or waxes, the high-temperature bonding subprocess is compatible with the deltadoping process as well as with other CCD-fabrication processes. The present improved process and its bonding, thinning, and delta-doping subprocesses, are characterized as postfabrication processes because they are undertaken after the fabrication of CCD circuitry on the front side of a full-thickness silicon substrate. In a typical case, it is necessary to reduce the thickness of the CCD to between 10 and 20 m in order to take advantage of back-side illumination and in order to perform delta doping and/or other back-side treatment to enhance the quantum efficiency. In the prior approach to the fabrication of back-side-illuminated CCDs, the thinning subprocess turned each CCD into a free-standing membrane that was fragile and tended to become wrinkled. In the present improved process, prior to thinning and delta doping, a CCD is bonded on its front side to a silicon substrate that has been prefabricated to include cutouts to accommodate subsequent electrical connections to bonding pads on the CCD circuitry. The substrate provides structural support to increase ruggedness and maintain flatness. At the beginning of this process, the back side of a CCD as fabricated on a full-thickness substrate is polished. Silicon nitride is deposited on the back side, opposite the bonding pads on the front side, in order to define a relatively thick frame. The portion of the CCD not covered by the frame is the portion to be thinned by etching.

A comparison study on flesh quality of large yellow croaker ( Larimichthys croceus) cultured with three different modes

NASA Astrophysics Data System (ADS)

Li, Xiaoqin; Li, Baian; Chen, Naisong; Huang, Xuxiong; Hua, Xuemin; Leng, Xiangjun

2017-12-01

To compare the flesh quality of large yellow croaker cultured with three different modes, enclosure culture (EC), cage culture with feeding trash fish (CCF) and cage culture with feeding formula diet (CCD), thirty six fish individuals of EC (484.6 ± 79.8 g), CCF (432.7 ± 87.9 g) and CCD (416.9 ± 49.5 g) were sampled to measure body color, flesh water holding capacity, flesh proximate composition, amino acids and fatty acids composition, and collagen and inosinic acid contents. The analysis showed that there was no significant difference in the yellowness of skin and the contents of crude protein and inosinic acid between CCD and EC (P >0.05), and the two modes showed higher values than CCF ( P < 0.05). Most of the amino acids (AA) in flesh were higher in EC than in CCF ( P < 0.05), but CCD showed no difference in AA composition from the other two modes ( P > 0.05). EC and CCD had higher contents of collagen, free AA and lower drip loss than CC ( P < 0.05). In polyunsaturated fatty acids, EC showed significantly higher levels of C20:5n-3 and C22:6n-3 than CCF and CCD ( P < 0.05), but CCD had significantly higher C18:2n-6 than CCF and EC ( P < 0.05). In conclusion, the flesh quality of CCD fish is similar to EC fish, and these two modes have better flesh quality than CCF.

Synergistic effect of dicarbollide anions in liquid-liquid extraction: a molecular dynamics study at the octanol-water interface.

PubMed

Chevrot, G; Schurhammer, R; Wipff, G

2007-04-28

We report a molecular dynamics study of chlorinated cobalt bis(dicarbollide) anions [(B(9)C(2)H(8)Cl(3))(2)Co](-)"CCD(-)" in octanol and at the octanol-water interface, with the main aim to understand why these hydrophobic species act as strong synergists in assisted liquid-liquid cation extraction. Neat octanol is quite heterogeneous and is found to display dual solvation properties, allowing to well solubilize CCD(-), Cs(+) salts in the form of diluted pairs or oligomers, without displaying aggregation. At the aqueous interface, octanol behaves as an amphiphile, forming either monolayers or bilayers, depending on the initial state and confinement conditions. In biphasic octanol-water systems, CCD(-) anions are found to mainly partition to the organic phase, thus attracting Cs(+) or even more hydrophilic counterions like Eu(3+) into that phase. The remaining CCD(-) anions adsorb at the interface, but are less surface active than at the chloroform interface. Finally, we compare the interfacial behavior of the Eu(BTP)(3)(3+) complex in the absence and in the presence of CCD(-) anions and extractant molecules. It is found that when the CCD(-)'s are concentrated enough, the complex is extracted to the octanol phase. Otherwise, it is trapped at the interface, attracted by water. These results are compared to those obtained with chloroform as organic phase and discussed in the context of synergistic effect of CCD(-) in liquid-liquid extraction, pointing to the importance of dual solvation properties of octanol and of the hydrophobic character of CCD(-) for synergistic extraction of cations.

Behavior of chemicals in the seawater column by shadowscopy

NASA Astrophysics Data System (ADS)

Fuhrer, Mélanie; Aprin, Laurent; Le Floch, Stéphane; Slangen, Pierre; Dusserre, Gilles

2012-10-01

Ninety percent of the Global Movement of Goods transit by ship. The transportation of HNS (Hazardous and Noxious Substances) in bulk highly increases with the tanker traffic. The huge volume capacities induce a major risk of accident involving chemicals. Among the latest accidents, many have led to vessels sinking (Ievoli Sun, 2000 - ECE, 2006). In case of floating substances, liquid release in depth entails an ascending two phase flow. The visualization of that flow is complex. Indeed, liquid chemicals have mostly a refractive index close to water, causing difficulties for the assessment of the two phase medium behavior. Several physics aspects are points of interest: droplets characterization (shape evolution and velocity), dissolution kinetics and hydrodynamic vortices. Previous works, presented in the 2010 Speckle conference in Brazil, employed Dynamic Speckle Interferometry to study Methyl Ethyl Ketone (MEK) dissolution in a 15 cm high and 1 cm thick water column. This paper deals with experiments achieved with the Cedre Experimental Column (CEC - 5 m high and 0.8 m in diameter). As the water thickness has been increased, Dynamic Speckle Interferometry results are improved by shadowscopic measurements. A laser diode is used to generate parallel light while high speed imaging records the products rising. Two measurements systems are placed at the bottom and the top of the CEC. The chemical class of pollutant like floaters, dissolvers (plume, trails or droplets) has been then identified. Physics of the two phase flow is presented and shows up the dependence on chemicals properties such as interfacial tension, viscosity and density. Furthermore, parallel light propagation through this disturbed medium has revealed trailing edges vortices for some substances (e.g. butanol) presenting low refractive index changes.

Bio-inspired multi-mode optic flow sensors for micro air vehicles

NASA Astrophysics Data System (ADS)

Park, Seokjun; Choi, Jaehyuk; Cho, Jihyun; Yoon, Euisik

2013-06-01

Monitoring wide-field surrounding information is essential for vision-based autonomous navigation in micro-air-vehicles (MAV). Our image-cube (iCube) module, which consists of multiple sensors that are facing different angles in 3-D space, can be applied to the wide-field of view optic flows estimation (μ-Compound eyes) and to attitude control (μ- Ocelli) in the Micro Autonomous Systems and Technology (MAST) platforms. In this paper, we report an analog/digital (A/D) mixed-mode optic-flow sensor, which generates both optic flows and normal images in different modes for μ- Compound eyes and μ-Ocelli applications. The sensor employs a time-stamp based optic flow algorithm which is modified from the conventional EMD (Elementary Motion Detector) algorithm to give an optimum partitioning of hardware blocks in analog and digital domains as well as adequate allocation of pixel-level, column-parallel, and chip-level signal processing. Temporal filtering, which may require huge hardware resources if implemented in digital domain, is remained in a pixel-level analog processing unit. The rest of the blocks, including feature detection and timestamp latching, are implemented using digital circuits in a column-parallel processing unit. Finally, time-stamp information is decoded into velocity from look-up tables, multiplications, and simple subtraction circuits in a chip-level processing unit, thus significantly reducing core digital processing power consumption. In the normal image mode, the sensor generates 8-b digital images using single slope ADCs in the column unit. In the optic flow mode, the sensor estimates 8-b 1-D optic flows from the integrated mixed-mode algorithm core and 2-D optic flows with an external timestamp processing, respectively.

Multi-modal diffuse optical techniques for breast cancer neoadjuvant chemotherapy monitoring (Conference Presentation)

NASA Astrophysics Data System (ADS)

Cochran, Jeffrey M.; Busch, David R.; Ban, Han Y.; Kavuri, Venkaiah C.; Schweiger, Martin J.; Arridge, Simon R.; Yodh, Arjun G.

2017-02-01

We present high spatial density, multi-modal, parallel-plate Diffuse Optical Tomography (DOT) imaging systems for the purpose of breast tumor detection. One hybrid instrument provides time domain (TD) and continuous wave (CW) DOT at 64 source fiber positions. The TD diffuse optical spectroscopy with PMT- detection produces low-resolution images of absolute tissue scattering and absorption while the spatially dense array of CCD-coupled detector fibers (108 detectors) provides higher-resolution CW images of relative tissue optical properties. Reconstruction of the tissue optical properties, along with total hemoglobin concentration and tissue oxygen saturation, is performed using the TOAST software suite. Comparison of the spatially-dense DOT images and MR images allows for a robust validation of DOT against an accepted clinical modality. Additionally, the structural information from co-registered MR images is used as a spatial prior to improve the quality of the functional optical images and provide more accurate quantification of the optical and hemodynamic properties of tumors. We also present an optical-only imaging system that provides frequency domain (FD) DOT at 209 source positions with full CCD detection and incorporates optical fringe projection profilometry to determine the breast boundary. This profilometry serves as a spatial constraint, improving the quality of the DOT reconstructions while retaining the benefits of an optical-only device. We present initial images from both human subjects and phantoms to display the utility of high spatial density data and multi-modal information in DOT reconstruction with the two systems.

KWFC: four square degrees camera for the Kiso Schmidt Telescope

NASA Astrophysics Data System (ADS)

Sako, Shigeyuki; Aoki, Tsutomu; Doi, Mamoru; Ienaka, Nobuyuki; Kobayashi, Naoto; Matsunaga, Noriyuki; Mito, Hiroyuki; Miyata, Takashi; Morokuma, Tomoki; Nakada, Yoshikazu; Soyano, Takao; Tarusawa, Ken'ichi; Miyazaki, Satoshi; Nakata, Fumiaki; Okada, Norio; Sarugaku, Yuki; Richmond, Michael W.

2012-09-01

The Kiso Wide Field Camera (KWFC) is a facility instrument for the 105-cm Schmidt telescope being operated by the Kiso Observatory of the University of Tokyo. This camera has been designed for wide-field observations by taking advantage of a large focal-plane area of the Schmidt telescope. Eight CCD chips with a total of 8k x 8k pixels cover a field-of-view of 2.2 degrees x 2.2 degrees on the sky. The dewar window works as a field flattener lens minimizing an image distortion across the field of view. Two shutter plates moving in parallel achieve uniform exposures on all the CCD pixels. The KWFC is equipped with a filter exchanger composed of an industrial robotic arm, a filter magazine capable of storing 12 filters, and a filter holder at the focal plane. Both the arm and the magazine are installed inside the tube framework of the telescope but without vignetting the beam. Wide-field survey programs searching for supernovae and late-type variable stars have begun in April 2012. The survey observations are performed with a management software system for facility instruments including the telescope and the KWFC. This system automatically carries out observations based on target lists registered in advance and makes appropriate decisions for implementation of observations by referring to weather conditions and status of the instruments. Image data obtained in the surveys are processed with pipeline software in real time to search for candidates of time-variable sources.

Findings toward the miniaturization of a laser speckle contrast device for skin roughness measurements

NASA Astrophysics Data System (ADS)

Louie, Daniel C.; Tchvialeva, Lioudmilla; Zeng, Haishan; Lee, Tim K.

2017-02-01

Skin roughness is an important parameter in the characterization of skin and skin lesions, particularly for the purposes of skin cancer detection. Our group had previously constructed a laser speckle device that can detect the roughness in microrelief of the skin. This paper reports on findings made for the further miniaturization of our existing portably-sized device. These findings include the feasibility of adopting a laser diode without temperature control, and the use of a single CCD camera for detection. The coherence length of a laser is a crucial criterion for speckle measurements as it must be within a specific range. The coherence length of a commercial grade 405 nm laser diode was found to be of an appropriate length. Also, after a short warm-up period the coherence length of the laser was found to remain relatively stable, even without temperature control. Although the laser's temperature change during operation may affect its power output and the shape of its spectrum, these are only minor factors in speckle contrast measurements. Our second finding covers a calibration curve to relate speckle measurements to roughness using only parallel polarization from one CCD camera. This was created using experimental data from skin phantoms and tested on in-vivo skin. These improvements are important steps forward in the ongoing development of the laser speckle device, especially towards a clinical device to measure skin roughness and evaluate skin lesions.

GPU acceleration of a petascale application for turbulent mixing at high Schmidt number using OpenMP 4.5

NASA Astrophysics Data System (ADS)

Clay, M. P.; Buaria, D.; Yeung, P. K.; Gotoh, T.

2018-07-01

This paper reports on the successful implementation of a massively parallel GPU-accelerated algorithm for the direct numerical simulation of turbulent mixing at high Schmidt number. The work stems from a recent development (Comput. Phys. Commun., vol. 219, 2017, 313-328), in which a low-communication algorithm was shown to attain high degrees of scalability on the Cray XE6 architecture when overlapping communication and computation via dedicated communication threads. An even higher level of performance has now been achieved using OpenMP 4.5 on the Cray XK7 architecture, where on each node the 16 integer cores of an AMD Interlagos processor share a single Nvidia K20X GPU accelerator. In the new algorithm, data movements are minimized by performing virtually all of the intensive scalar field computations in the form of combined compact finite difference (CCD) operations on the GPUs. A memory layout in departure from usual practices is found to provide much better performance for a specific kernel required to apply the CCD scheme. Asynchronous execution enabled by adding the OpenMP 4.5 NOWAIT clause to TARGET constructs improves scalability when used to overlap computation on the GPUs with computation and communication on the CPUs. On the 27-petaflops supercomputer Titan at Oak Ridge National Laboratory, USA, a GPU-to-CPU speedup factor of approximately 5 is consistently observed at the largest problem size of 81923 grid points for the scalar field computed with 8192 XK7 nodes.

A Real-Time Position-Locating Algorithm for CCD-Based Sunspot Tracking

NASA Technical Reports Server (NTRS)

Taylor, Jaime R.

1996-01-01

NASA Marshall Space Flight Center's (MSFC) EXperimental Vector Magnetograph (EXVM) polarimeter measures the sun's vector magnetic field. These measurements are taken to improve understanding of the sun's magnetic field in the hopes to better predict solar flares. Part of the procedure for the EXVM requires image motion stabilization over a period of a few minutes. A high speed tracker can be used to reduce image motion produced by wind loading on the EXVM, fluctuations in the atmosphere and other vibrations. The tracker consists of two elements, an image motion detector and a control system. The image motion detector determines the image movement from one frame to the next and sends an error signal to the control system. For the ground based application to reduce image motion due to atmospheric fluctuations requires an error determination at the rate of at least 100 hz. It would be desirable to have an error determination rate of 1 kHz to assure that higher rate image motion is reduced and to increase the control system stability. Two algorithms are presented that are typically used for tracking. These algorithms are examined for their applicability for tracking sunspots, specifically their accuracy if only one column and one row of CCD pixels are used. To examine the accuracy of this method two techniques are used. One involves moving a sunspot image a known distance with computer software, then applying the particular algorithm to see how accurately it determines this movement. The second technique involves using a rate table to control the object motion, then applying the algorithms to see how accurately each determines the actual motion. Results from these two techniques are presented.

Study of physical and sound absorbing property of epoxy blended coir dust biocomposite

NASA Astrophysics Data System (ADS)

Nath, G.; Mishra, S. P.

2016-09-01

Reinforcement biocomposite has gained more attention recently due to its low cost, abundantly availability, low density, specific properties, easy method of separation, enhanced energy recovery, CO2 neutrality, biodegradability and recyclable in nature. As a waste product of coconut fruit, the coconut coir dust (CCD) obtained from the coconut husk. The biocomposite material prepared from the CCD modified with the proper blended solution with the help of ultrasonic technique. The study of adiabatic compressibility of acetone / water (70/30) worth its blending property for bleaching of CCD. The biocomposite material of CCD was prepared with epoxy resin. The different physical properties such as sound absorption coefficient, thermal conductivity and electrical conductivity were measured. The morphological study of biocomposite and measurement of sound absorption coefficient shows good evidence of sound absorbing characteristics of biocomposite of CCD. The sound absorption property of composite material shows a significant result where as the thermal conductivity and electrical conductivity executes a weak result. Thus biocomposite of CCD can acts as a good sound absorber and band conductor of heat and electric current.

Driving techniques for high frame rate CCD camera

NASA Astrophysics Data System (ADS)

Guo, Weiqiang; Jin, Longxu; Xiong, Jingwu

2008-03-01

This paper describes a high-frame rate CCD camera capable of operating at 100 frames/s. This camera utilizes Kodak KAI-0340, an interline transfer CCD with 640(vertical)×480(horizontal) pixels. Two output ports are used to read out CCD data and pixel rates approaching 30 MHz. Because of its reduced effective opacity of vertical charge transfer registers, interline transfer CCD can cause undesired image artifacts, such as random white spots and smear generated in the registers. To increase frame rate, a kind of speed-up structure has been incorporated inside KAI-0340, then it is vulnerable to a vertical stripe effect. The phenomena which mentioned above may severely impair the image quality. To solve these problems, some electronic methods of eliminating these artifacts are adopted. Special clocking mode can dump the unwanted charge quickly, then the fast readout of the images, cleared of smear, follows immediately. Amplifier is used to sense and correct delay mismatch between the dual phase vertical clock pulses, the transition edges become close to coincident, so vertical stripes disappear. Results obtained with the CCD camera are shown.

Linking Spectral Induced Polarization (SIP) and Subsurface Microbial Processes: Results from Sand Column Incubation Experiments.

PubMed

Mellage, Adrian; Smeaton, Christina M; Furman, Alex; Atekwana, Estella A; Rezanezhad, Fereidoun; Van Cappellen, Philippe

2018-02-20

Geophysical techniques, such as spectral induced polarization (SIP), offer potentially powerful approaches for in situ monitoring of subsurface biogeochemistry. The successful implementation of these techniques as monitoring tools for reactive transport phenomena, however, requires the deconvolution of multiple contributions to measured signals. Here, we present SIP spectra and complementary biogeochemical data obtained in saturated columns packed with alternating layers of ferrihydrite-coated and pure quartz sand, and inoculated with Shewanella oneidensis supplemented with lactate and nitrate. A biomass-explicit diffusion-reaction model is fitted to the experimental biogeochemical data. Overall, the results highlight that (1) the temporal response of the measured imaginary conductivity peaks parallels the microbial growth and decay dynamics in the columns, and (2) SIP is sensitive to changes in microbial abundance and cell surface charging properties, even at relatively low cell densities (<10 8 cells mL -1 ). Relaxation times (τ) derived using the Cole-Cole model vary with the dominant electron accepting process, nitrate or ferric iron reduction. The observed range of τ values, 0.012-0.107 s, yields effective polarization diameters in the range 1-3 μm, that is, 2 orders of magnitude smaller than the smallest quartz grains in the columns, suggesting that polarization of the bacterial cells controls the observed chargeability and relaxation dynamics in the experiments.

A 128K-bit CCD buffer memory system

NASA Technical Reports Server (NTRS)

Siemens, K. H.; Wallace, R. W.; Robinson, C. R.

1976-01-01

A prototype system was implemented to demonstrate that CCD's can be applied advantageously to the problem of low power digital storage and particularly to the problem of interfacing widely varying data rates. 8K-bit CCD shift register memories were used to construct a feasibility model 128K-bit buffer memory system. Peak power dissipation during a data transfer is less than 7 W., while idle power is approximately 5.4 W. The system features automatic data input synchronization with the recirculating CCD memory block start address. Descriptions are provided of both the buffer memory system and a custom tester that was used to exercise the memory. The testing procedures and testing results are discussed. Suggestions are provided for further development with regards to the utilization of advanced versions of CCD memory devices to both simplified and expanded memory system applications.

Assessment on the leakage hazard of landfill leachate using three-dimensional excitation-emission fluorescence and parallel factor analysis method.

PubMed

Pan, Hongwei; Lei, Hongjun; Liu, Xin; Wei, Huaibin; Liu, Shufang

2017-09-01

A large number of simple and informal landfills exist in developing countries, which pose as tremendous soil and groundwater pollution threats. Early warning and monitoring of landfill leachate pollution status is of great importance. However, there is a shortage of affordable and effective tools and methods. In this study, a soil column experiment was performed to simulate the pollution status of leachate using three-dimensional excitation-emission fluorescence (3D-EEMF) and parallel factor analysis (PARAFAC) models. Sum of squared residuals (SSR) and principal component analysis (PCA) were used to determine the optimal components for PARAFAC. A one-way analysis of variance showed that the component scores of the soil column leachate were significant influenced by landfill leachate (p<0.05). Therefore, the ratio of the component scores of the soil under the landfill to that of natural soil could be used to evaluate the leakage status of landfill leachate. Furthermore, a hazard index (HI) and a hazard evaluation standard were established. A case study of Kaifeng landfill indicated a low hazard (level 5) by the use of HI. In summation, HI is presented as a tool to evaluate landfill pollution status and for the guidance of municipal solid waste management. Copyright © 2017 Elsevier Ltd. All rights reserved.

Application of Intel Many Integrated Core (MIC) architecture to the Yonsei University planetary boundary layer scheme in Weather Research and Forecasting model

NASA Astrophysics Data System (ADS)

Huang, Melin; Huang, Bormin; Huang, Allen H.

2014-10-01

The Weather Research and Forecasting (WRF) model provided operational services worldwide in many areas and has linked to our daily activity, in particular during severe weather events. The scheme of Yonsei University (YSU) is one of planetary boundary layer (PBL) models in WRF. The PBL is responsible for vertical sub-grid-scale fluxes due to eddy transports in the whole atmospheric column, determines the flux profiles within the well-mixed boundary layer and the stable layer, and thus provide atmospheric tendencies of temperature, moisture (including clouds), and horizontal momentum in the entire atmospheric column. The YSU scheme is very suitable for massively parallel computation as there are no interactions among horizontal grid points. To accelerate the computation process of the YSU scheme, we employ Intel Many Integrated Core (MIC) Architecture as it is a multiprocessor computer structure with merits of efficient parallelization and vectorization essentials. Our results show that the MIC-based optimization improved the performance of the first version of multi-threaded code on Xeon Phi 5110P by a factor of 2.4x. Furthermore, the same CPU-based optimizations improved the performance on Intel Xeon E5-2603 by a factor of 1.6x as compared to the first version of multi-threaded code.

A Fast Multiple Sampling Method for Low-Noise CMOS Image Sensors With Column-Parallel 12-bit SAR ADCs.

PubMed

Kim, Min-Kyu; Hong, Seong-Kwan; Kwon, Oh-Kyong

2015-12-26

This paper presents a fast multiple sampling method for low-noise CMOS image sensor (CIS) applications with column-parallel successive approximation register analog-to-digital converters (SAR ADCs). The 12-bit SAR ADC using the proposed multiple sampling method decreases the A/D conversion time by repeatedly converting a pixel output to 4-bit after the first 12-bit A/D conversion, reducing noise of the CIS by one over the square root of the number of samplings. The area of the 12-bit SAR ADC is reduced by using a 10-bit capacitor digital-to-analog converter (DAC) with four scaled reference voltages. In addition, a simple up/down counter-based digital processing logic is proposed to perform complex calculations for multiple sampling and digital correlated double sampling. To verify the proposed multiple sampling method, a 256 × 128 pixel array CIS with 12-bit SAR ADCs was fabricated using 0.18 μm CMOS process. The measurement results shows that the proposed multiple sampling method reduces each A/D conversion time from 1.2 μs to 0.45 μs and random noise from 848.3 μV to 270.4 μV, achieving a dynamic range of 68.1 dB and an SNR of 39.2 dB.

Care for Child Development: an intervention in support of responsive caregiving and early child development.

PubMed

Lucas, J E; Richter, L M; Daelmans, B

2018-01-01

An estimated 43% of children younger than 5 years of age are at elevated risk of failing to achieve their human potential. In response, the World Health Organization and UNICEF developed Care for Child Development (CCD), based on the science of child development, to improve sensitive and responsive caregiving and promote the psychosocial development of young children. In 2015, the World Health Organization and UNICEF identified sites where CCD has been implemented and sustained. The sites were surveyed, and responses were followed up by phone interviews. Project reports provided information on additional sites, and a review of published studies was undertaken to document the effectiveness of CCD for improving child and family outcomes, as well as its feasibility for implementation in resource-constrained communities. The inventory found that CCD had been integrated into existing services in diverse sectors in 19 countries and 23 sites, including child survival, health, nutrition, infant day care, early education, family and child protection and services for children with disabilities. Published and unpublished evaluations have found that CCD interventions can improve child development, growth and health, as well as responsive caregiving. It has also been reported to reduce maternal depression, a known risk factor for poor pregnancy outcomes and poor child health, growth and development. Although CCD has expanded beyond initial implementation sites, only three countries reported having national policy support for integrating CCD into health or other services. Strong interest exists in many countries to move beyond child survival to protect and support optimal child development. The United Nations Sustainable Development Goals depend on children realizing their potential to build healthy and emotionally, cognitively and socially competent future generations. More studies are needed to guide the integration of the CCD approach under different conditions. Nevertheless, the time is right to provide for the scale-up of CCD as part of services for families and children. © 2017 The Authors. Child: Care, Health and Development Published by John Wiley & Sons Ltd.

Experimental research on thermal conductive fillers for CCD module in space borne optical remote sensor

NASA Astrophysics Data System (ADS)

Zeng, Yi; Han, Xue-bing; Yang, Dong-shang; Gui, Li-jia; Zhao, Xiao-xiang; Si, Fu-qi

2016-03-01

A space-borne differential optical absorption spectrometer is a high precision aerospace optical remote sensor. It obtains the hyper-spectral，high spatial resolution radiation information by using the spectrometer with CCD（Charge Coupled Device）array detectors. Since a few CCDs are used as the key detector, the performance of the entire instrument is greatly affected by working condition of CCDs. The temperature of CCD modules has a great impact on the instrument measurement accuracy. It requires strict temperature control. The selection of the thermal conductive filler sticking CCD to the radiator is important in the CCD thermal design. Besides，due tothe complex and compact structure, it needs to take into account the anti-pollution of the optical system. Therefore, it puts forward high requirements on the selection of the conductive filler. In this paper, according to the structure characteristics of the CCD modules and the distribution of heat consumption, the thermal analysis tool I-DEAS/TMG is utilized to compute and simulate the temperature level of the CCD modules, while filling in thermal grease and thermal pad respectively. The temperature distribution of CCD heat dissipation in typical operating conditions is obtained. In addition, the heat balance test was carried out under the condition of two kinds of thermal conductive fillers. The thermal control of CCD was tested under various conditions, and the results were compared with the results of thermal analysis. The results show that there are some differences in thermal performance between the two kinds of thermal conductive fillers. Although they both can meet the thermal performance requirements of the instrument, either would be chosen taking account of other conditions and requirements such as anti-pollution and insulation. The content and results of this paper will be a good reference for the thermal design of the CCD in the aerospace optical payload.

Evaporation From Soil Containers With Irregular Shapes

NASA Astrophysics Data System (ADS)

Assouline, Shmuel; Narkis, Kfir

2017-11-01

Evaporation from bare soils under laboratory conditions is generally studied using containers of regular shapes where the vertical edges are parallel to the flow lines in the drying domain. The main objective of this study was to investigate the impact of irregular container shapes, for which the flow lines either converge or diverge toward the surface. Evaporation from initially saturated sand and sandy loam soils packed in cones and inverted cones was compared to evaporation from corresponding cylindrical columns. The initial evaporation rate was higher in the cones, and close to potential evaporation. At the end of the experiment, the cumulative evaporation depth in the sand cone was equal to that in the column but higher than in the inverted cone, while in the sandy loam, the order was cone > column > inverted cone. By comparison to the column, stage 1 evaporation was longer in the cones, and practically similar in the inverted cones. Stage 2 evaporation rate decreased with the increase of the evaporating surface area. These results were more pronounced in the sandy loam. For the sand column, the transition between stage 1 and stage 2 evaporation occurred when the depth of the saturation front was approximately equal to the characteristic length of the soil. However, for the cone and the inverted cone, it occurred for a shallower depth of the saturation front. It seems therefore that the concept of the characteristic length derived from the soil hydraulic properties is related to drying systems of regular shapes.

Toolkit for testing scientific CCD cameras

NASA Astrophysics Data System (ADS)

Uzycki, Janusz; Mankiewicz, Lech; Molak, Marcin; Wrochna, Grzegorz

2006-03-01

The CCD Toolkit (1) is a software tool for testing CCD cameras which allows to measure important characteristics of a camera like readout noise, total gain, dark current, 'hot' pixels, useful area, etc. The application makes a statistical analysis of images saved in files with FITS format, commonly used in astronomy. A graphical interface is based on the ROOT package, which offers high functionality and flexibility. The program was developed in a way to ensure future compatibility with different operating systems: Windows and Linux. The CCD Toolkit was created for the "Pie of the Sky" project collaboration (2).

Low temperature multi-alkali photocathode processing technique for sealed intensified CCD tubes

NASA Technical Reports Server (NTRS)

Doliber, D. L.; Dozier, E. E.; Wenzel, H.; Beaver, E. A.; Hier, R. G.

1989-01-01

A low temperature photocathode process has been used to fabricate an intensified CCD visual photocathode image tube, by incorporating a thinned, backside-illumined CCD as the target anode of a digicon tube of Hubble Space Telescope (HST) design. The CCD digicon tube employs the HST's sodium bialkali photocathode and MgF2 substrate, thereby allowing a direct photocathode quantum efficiency comparison between photocathodes produced by the presently employed low temperature process and those of the conventional high temperature process. Attention is given to the processing chamber used, as well as the details of gas desorption and photocathode processing.

Interpretation of TOMS Observations of Tropical Tropospheric Ozone with a Global Model and In Situ Observations

NASA Technical Reports Server (NTRS)

Martin, Randall V.; Jacob, Daniel J.; Logan, Jennifer A.; Bey, Isabelle; Yantosca, Robert M.; Staudt, Amanda C.; Fiore, Arlene M.; Duncan, Bryan N.; Liu, Hongyu; Ginoux, Paul

2004-01-01

We interpret the distribution of tropical tropospheric ozone columns (TTOCs) from the Total Ozone Mapping Spectrometer (TOMS) by using a global three-dimensional model of tropospheric chemistry (GEOS-CHEM) and additional information from in situ observations. The GEOS-CHEM TTOCs capture 44% of the variance of monthly mean TOMS TTOCs from the convective cloud differential method (CCD) with no global bias. Major discrepancies are found over northern Africa and south Asia where the TOMS TTOCs do not capture the seasonal enhancements from biomass burning found in the model and in aircraft observations. A characteristic feature of these northern topical enhancements, in contrast to southern tropical enhancements, is that they are driven by the lower troposphere where the sensitivity of TOMS is poor due to Rayleigh scattering. We develop an efficiency correction to the TOMS retrieval algorithm that accounts for the variability of ozone in the lower troposphere. This efficiency correction increases TTOC's over biomass burning regions by 3-5 Dobson units (DU) and decreases them by 2-5 DU over oceanic regions, improving the agreement between CCD TTOCs and in situ observations. Applying the correction to CCD TTOCs reduces by approximately DU the magnitude of the "tropical Atlantic paradox" [Thompson et al, 2000], i.e. the presence of a TTOC enhancement over the southern tropical Atlantic during the northern African biomass burning season in December-February. We reproduce the remainder of the paradox in the model and explain it by the combination of upper tropospheric ozone production from lightning NOx, peristent subsidence over the southern tropical Atlantic as part of the Walker circulation, and cross-equatorial transport of upper tropospheric ozone from northern midlatitudes in the African "westerly duct." These processes in the model can also account for the observed 13-17 DU persistent wave-1 pattern in TTOCs with a maximum above the tropical Atlantic and a minimum over the tropical Pacific during all seasons. The photochemical effects of mineral dust have only a minor role on the modeled distribution of TTOCs, including over northern Africa, due to multiple competing effects. The photochemical effects of mineral dust globally decease annual mean OH concentrations by 9%. A global lightning NOx source of 6 Tg N yr(sup -1) in the model produces a simulation that is most consistent with TOMS and in situ observations.

Optimization by response surface methodology of lutein recovery from paprika leaves using accelerated solvent extraction.

PubMed

Kang, Jae-Hyun; Kim, Suna; Moon, BoKyung

2016-08-15

In this study, we used response surface methodology (RSM) to optimize the extraction conditions for recovering lutein from paprika leaves using accelerated solvent extraction (ASE). The lutein content was quantitatively analyzed using a UPLC equipped with a BEH C18 column. A central composite design (CCD) was employed for experimental design to obtain the optimized combination of extraction temperature (°C), static time (min), and solvent (EtOH, %). The experimental data obtained from a twenty sample set were fitted to a second-order polynomial equation using multiple regression analysis. The adjusted coefficient of determination (R(2)) for the lutein extraction model was 0.9518, and the probability value (p=0.0000) demonstrated a high significance for the regression model. The optimum extraction conditions for lutein were temperature: 93.26°C, static time: 5 min, and solvent: 79.63% EtOH. Under these conditions, the predicted extraction yield of lutein was 232.60 μg/g. Copyright © 2016 Elsevier Ltd. All rights reserved.

Regulation of transport in the connecting tubule and cortical collecting duct.

PubMed

Staruschenko, Alexander

2012-04-01

The central goal of this overview article is to summarize recent findings in renal epithelial transport,focusing chiefly on the connecting tubule (CNT) and the cortical collecting duct (CCD).Mammalian CCD and CNT are involved in fine-tuning of electrolyte and fluid balance through reabsorption and secretion. Specific transporters and channels mediate vectorial movements of water and solutes in these segments. Although only a small percent of the glomerular filtrate reaches the CNT and CCD, these segments are critical for water and electrolyte homeostasis since several hormones, for example, aldosterone and arginine vasopressin, exert their main effects in these nephron sites. Importantly, hormones regulate the function of the entire nephron and kidney by affecting channels and transporters in the CNT and CCD. Knowledge about the physiological and pathophysiological regulation of transport in the CNT and CCD and particular roles of specific channels/transporters has increased tremendously over the last two decades.Recent studies shed new light on several key questions concerning the regulation of renal transport.Precise distribution patterns of transport proteins in the CCD and CNT will be reviewed, and their physiological roles and mechanisms mediating ion transport in these segments will also be covered. Special emphasis will be given to pathophysiological conditions appearing as a result of abnormalities in renal transport in the CNT and CCD. © 2012 American Physiological Society. Compr Physiol 2:1491-1539, 2012.

Cervical spine dysfunction signs and symptoms in individuals with temporomandibular disorder.

PubMed

Weber, Priscila; Corrêa, Eliane Castilhos Rodrigues; Ferreira, Fabiana dos Santos; Soares, Juliana Corrêa; Bolzan, Geovana de Paula; Silva, Ana Maria Toniolo da

2012-01-01

To study the frequency of cervical spine dysfunction (CCD) signs and symptoms in subjects with and without temporomandibular disorder (TMD) and to assess the craniocervical posture influence on TMD and CCD coexistence. Participants were 71 women (19 to 35 years), assessed about TMD presence; 34 constituted the TMD group (G1) and 37 comprised the group without TMD (G2). The CCD was evaluated through the Craniocervical Dysfunction Index and the Cervical Mobility Index. Subjects were also questioned about cervical pain. Craniocervical posture was assessed by cephalometric analysis. There was no difference in the craniocervical posture between groups. G2 presented more mild CCD frequency and less moderate and severe CCD frequency (p=0.01). G1 presented higher percentage of pain during movements (p=0.03) and pain during cervical muscles palpation (p=0.01) compared to G2. Most of the TMD patients (88.24%) related cervical pain with significant difference when compared to G2 (p=0.00). Craniocervical posture assessment showed no difference between groups, suggesting that postural alterations could be more related to the CCD. Presence of TMD resulted in higher frequency of cervical pain symptom. Thus the coexistence of CCD and TMD signs and symptoms appear to be more related to the common innervations of the trigeminocervical complex and hyperalgesia of the TMD patients than to craniocervical posture deviations.

Instructional Staff Salary and Benefits Spending: 1991-2011. Data Point. NCES 2016-156

ERIC Educational Resources Information Center

Malkus, Nat; Hoyer, Kathleen Mulvaney

2016-01-01

This report uses expenditure data from the Common Core of Data (CCD) "National Public Education Financial Survey (NPEFS)" for fiscal years 1991 to 2011 and instructional staff data from the CCD "State Nonfiscal Survey of Public Elementary/Secondary Education" for school years 1990-91 to 2010-11. The CCD annually collects fiscal…

Optical Remote Sensing Measurements of Air Pollution in Mexico City During MCMA- 2006

NASA Astrophysics Data System (ADS)

Galle, B.; Mellqvist, J.; Johansson, M.; Rivera, C.; Samuelsson, J.; Zhang, Y.

2007-05-01

During March 2006 the Optical Remote sensing group at Chalmers University of Technology participated in the MCMA-2006 field campaign in Mexico City, performing measurements of air pollution using a set of different optical remote sensing instruments. This poster gives an overview of the techniques applied and results obtained. The techniques applied were: Solar Occultation FTIR and UV spectroscopy from fixed locations throughout the MCMA area, yielding total columns of CO, CH2O, SO2 and NO2. Long Path FTIR measurements from site T0 located in the north part of central Mexico City. With this instrument line-averaged concentration measurements of CO and CO2 was obtained in parallel with DOAS measurements performed by other partners. MAX-DOAS measurements from site T0, yielding total column and spatial distributions of SO2 and NO2. Mobile DOAS scattered Sunlight measurements of total columns of SO2 and NO2 in and around the MCMA area. Mobile and stationary DOAS measurements in the vicinity of Tula and Popocatépetl in order to quantify emissions from industry and volcano.

[Techniques for pixel response nonuniformity correction of CCD in interferential imaging spectrometer].

PubMed

Yao, Tao; Yin, Shi-Min; Xiangli, Bin; Lü, Qun-Bo

2010-06-01

Based on in-depth analysis of the relative radiation scaling theorem and acquired scaling data of pixel response nonuniformity correction of CCD (charge-coupled device) in spaceborne visible interferential imaging spectrometer, a pixel response nonuniformity correction method of CCD adapted to visible and infrared interferential imaging spectrometer system was studied out, and it availably resolved the engineering technical problem of nonuniformity correction in detector arrays for interferential imaging spectrometer system. The quantitative impact of CCD nonuniformity on interferogram correction and recovery spectrum accuracy was given simultaneously. Furthermore, an improved method with calibration and nonuniformity correction done after the instrument is successfully assembled was proposed. The method can save time and manpower. It can correct nonuniformity caused by other reasons in spectrometer system besides CCD itself's nonuniformity, can acquire recalibration data when working environment is changed, and can also more effectively improve the nonuniformity calibration accuracy of interferential imaging

Familial cleidocranial dysplasia misdiagnosed as rickets over three generations.

PubMed

Franceschi, Roberto; Maines, Evelina; Fedrizzi, Michela; Piemontese, Maria Rosaria; De Bonis, Patrizia; Agarwal, Nivedita; Bellizzi, Maria; Di Palma, Annunziata

2015-10-01

Cleidocranial dysplasia (CCD) is a rare autosomal dominant skeletal dysplasia characterized by hypoplastic clavicles, late closure of the fontanels, dental problems and other skeletal features. CCD is caused by mutations, deletions or duplications in runt-related transcription factor 2 (RUNX2), which encodes for a protein essential for osteoblast differentiation and chondrocyte maturation. We describe three familial cases of CCD, misdiagnosed as rickets over three generations. No mutations were detected on standard DNA sequencing of RUNX2, but a novel deletion was identified on quantitative polymerase chain reaction (qPCR) and multiple ligation-dependent probe amplification (MLPA). The present cases indicate that CCD could be misdiagnosed as rickets, leading to inappropriate treatment, and confirm that mutations in RUNX2 are not able to be identified on standard DNA sequencing in all CCD patients, but can be identified on qPCR and MLPA. © 2015 Japan Pediatric Society.

A CCD experimental platform for large telescope in Antarctica based on FPGA

NASA Astrophysics Data System (ADS)

Zhu, Yuhua; Qi, Yongjun

2014-07-01

The CCD , as a detector , is one of the important components of astronomical telescopes. For a large telescope in Antarctica, a set of CCD detector system with large size, high sensitivity and low noise is indispensable. Because of the extremely low temperatures and unattended, system maintenance and software and hardware upgrade become hard problems. This paper introduces a general CCD controller experiment platform, using Field programmable gate array FPGA, which is, in fact, a large-scale field reconfigurable array. Taking the advantage of convenience to modify the system, construction of driving circuit, digital signal processing module, network communication interface, control algorithm validation, and remote reconfigurable module may realize. With the concept of integrated hardware and software, the paper discusses the key technology of building scientific CCD system suitable for the special work environment in Antarctica, focusing on the method of remote reconfiguration for controller via network and then offering a feasible hardware and software solution.

High resolution biomedical imaging system with direct detection of x-rays via a charge coupled device

DOEpatents

Atac, M.; McKay, T.A.

1998-04-21

An imaging system is provided for direct detection of x-rays from an irradiated biological tissue. The imaging system includes an energy source for emitting x-rays toward the biological tissue and a charge coupled device (CCD) located immediately adjacent the biological tissue and arranged transverse to the direction of irradiation along which the x-rays travel. The CCD directly receives and detects the x-rays after passing through the biological tissue. The CCD is divided into a matrix of cells, each of which individually stores a count of x-rays directly detected by the cell. The imaging system further includes a pattern generator electrically coupled to the CCD for reading a count from each cell. A display device is provided for displaying an image representative of the count read by the pattern generator from the cells of the CCD. 13 figs.

High resolution biomedical imaging system with direct detection of x-rays via a charge coupled device

DOEpatents

Atac, Muzaffer; McKay, Timothy A.

1998-01-01

An imaging system is provided for direct detection of x-rays from an irradiated biological tissue. The imaging system includes an energy source for emitting x-rays toward the biological tissue and a charge coupled device (CCD) located immediately adjacent the biological tissue and arranged transverse to the direction of irradiation along which the x-rays travel. The CCD directly receives and detects the x-rays after passing through the biological tissue. The CCD is divided into a matrix of cells, each of which individually stores a count of x-rays directly detected by the cell. The imaging system further includes a pattern generator electrically coupled to the CCD for reading a count from each cell. A display device is provided for displaying an image representative of the count read by the pattern generator from the cells of the CCD.

Investigation of E. coli and Virus Reductions Using Replicate, Bench-Scale Biosand Filter Columns and Two Filter Media

PubMed Central

Elliott, Mark; Stauber, Christine E.; DiGiano, Francis A.; Fabiszewski de Aceituno, Anna; Sobsey, Mark D.

2015-01-01

The biosand filter (BSF) is an intermittently operated, household-scale slow sand filter for which little data are available on the effect of sand composition on treatment performance. Therefore, bench-scale columns were prepared according to the then-current (2006–2007) guidance on BSF design and run in parallel to conduct two microbial challenge experiments of eight-week duration. Triplicate columns were loaded with Accusand silica or crushed granite to compare virus and E. coli reduction performance. Bench-scale experiments provided confirmation that increased schmutzdecke growth, as indicated by decline in filtration rate, is the primary factor causing increased E. coli reductions of up to 5-log10. However, reductions of challenge viruses improved only modestly with increased schmutzdecke growth. Filter media type (Accusand silica vs. crushed granite) did not influence reduction of E. coli bacteria. The granite media without backwashing yielded superior virus reductions when compared to Accusand. However, for columns in which the granite media was first backwashed (to yield a more consistent distribution of grains and remove the finest size fraction), virus reductions were not significantly greater than in columns with Accusand media. It was postulated that a decline in surface area with backwashing decreased the sites and surface area available for virus sorption and/or biofilm growth and thus decreased the extent of virus reduction. Additionally, backwashing caused preferential flow paths and deviation from plug flow; backwashing is not part of standard BSF field preparation and is not recommended for BSF column studies. Overall, virus reductions were modest and did not meet the 5- or 3-log10 World Health Organization performance targets. PMID:26308036

Development of at-line assay to monitor charge variants of MAbs during production.

PubMed

St Amand, M M; Ogunnaike, B A; Robinson, A S

2014-01-01

One major challenge currently facing the biopharmaceutical industry is to understand how MAb microheterogeneity affects therapeutic efficacy, potency, immunogenicity, and clearance. MAb micro-heterogeneity can result from post-translational modifications such as sialylation, galactosylation, C-terminal lysine cleavage, glycine amidation, and tryptophan oxidation, each of which can generate MAb charge variants; such heterogeneity can affect pharmacokinetics (PK) considerably. Implementation of appropriate on-line quality control strategies may help to regulate bioprocesses, thus enabling more homogenous material with desired post-translational modifications and PK behavior. However, one major restriction to implementation of quality control strategies is the availability of techniques for obtaining on-line or at-line measurements of these attributes. In this work, we describe the development of an at-line assay to separate MAb charge variants in near real-time, which could ultimately be used to implement on-line quality control strategies for MAb production. The assay consists of a 2D-HPLC method with sequential in-line Protein A and WCX-10 HPLC column steps. To perform the 2D-HPLC assay at-line, the two columns steps were integrated into a single method using a novel system configuration that allowed parallel flow over column 1 or column 2 or sequential flow from column 1 to column 2. A bioreactor system was also developed such that media samples could be removed automatically from bioreactor vessels during production and delivered to the 2D-HPLC for analysis. With this at-line HPLC assay, we have demonstrated that MAb microheterogeneity occurs throughout the cell cycle whether the host cell line is grown under different or the same nominal culture conditions. © 2013 American Institute of Chemical Engineers.

IAU Working Group on Wide-Field Imaging.

NASA Astrophysics Data System (ADS)

MacGillivray, H. T.

1991-01-01

Contents: 1. Introduction - The IAU Working Group on Wide-Field Imaging (R. M. West). 2. Reports from the Sub-Sections of the Working Group - a. Sky surveys and patrols (R. M. West). b. Photographic techniques (D. F. Malin). c. Digitization techniques (H. T. MacGillivray). d. Archival and retrieval of wide-field data (B. Lasker). 3. Meeting of the Organising Committee (R. M. West). 4. Wide-field plate archives (M. Tsvetkov). 5. Reproduction of the Palomar Observatory Sky Surveys (R. J. Brucato). 6. Status of the St ScI scan-distribution program (B. Lasker). 7. Pixel addition - pushing Schmidt plates to B = 25 (M. R. S. Hawkins). 8. Photometry from Estar film (S. Phillipps, Q. Parker). 9. ASCHOT - Astrophysical Schmidt Orbital Telescope (H. Lorenz). 10. The Hitchhiker parallel CCD camera (J. Davies, M. Disney, S. Driver, I. Morgan, S. Phillipps).

Applying LED in full-field optical coherence tomography for gastrointestinal endoscopy

NASA Astrophysics Data System (ADS)

Yang, Bor-Wen; Wang, Yu-Yen; Juan, Yu-Shan; Hsu, Sheng-Jie

2015-08-01

Optical coherence tomography (OCT) has become an important medical imaging technology due to its non-invasiveness and high resolution. Full-field optical coherence tomography (FF-OCT) is a scanning scheme especially suitable for en face imaging as it employs a CMOS/CCD device for parallel pixels processing. FF-OCT can also be applied to high-speed endoscopic imaging. Applying cylindrical scanning and a right-angle prism, we successfully obtained a 360° tomography of the inner wall of an intestinal cavity through an FF-OCT system with an LED source. The 10-μm scale resolution enables the early detection of gastrointestinal lesions, which can increase detection rates for esophageal, stomach, or vaginal cancer. All devices used in this system can be integrated by MOEMS technology to contribute to the studies of gastrointestinal medicine and advanced endoscopy technology.

The Theory of Localist Representation and of a Purely Abstract Cognitive System: The Evidence from Cortical Columns, Category Cells, and Multisensory Neurons.

PubMed

Roy, Asim

2017-01-01

The debate about representation in the brain and the nature of the cognitive system has been going on for decades now. This paper examines the neurophysiological evidence, primarily from single cell recordings, to get a better perspective on both the issues. After an initial review of some basic concepts, the paper reviews the data from single cell recordings - in cortical columns and of category-selective and multisensory neurons. In neuroscience, columns in the neocortex (cortical columns) are understood to be a basic functional/computational unit. The paper reviews the fundamental discoveries about the columnar organization and finds that it reveals a massively parallel search mechanism. This columnar organization could be the most extensive neurophysiological evidence for the widespread use of localist representation in the brain. The paper also reviews studies of category-selective cells. The evidence for category-selective cells reveals that localist representation is also used to encode complex abstract concepts at the highest levels of processing in the brain. A third major issue is the nature of the cognitive system in the brain and whether there is a form that is purely abstract and encoded by single cells. To provide evidence for a single-cell based purely abstract cognitive system, the paper reviews some of the findings related to multisensory cells. It appears that there is widespread usage of multisensory cells in the brain in the same areas where sensory processing takes place. Plus there is evidence for abstract modality invariant cells at higher levels of cortical processing. Overall, that reveals the existence of a purely abstract cognitive system in the brain. The paper also argues that since there is no evidence for dense distributed representation and since sparse representation is actually used to encode memories, there is actually no evidence for distributed representation in the brain. Overall, it appears that, at an abstract level, the brain is a massively parallel, distributed computing system that is symbolic. The paper also explains how grounded cognition and other theories of the brain are fully compatible with localist representation and a purely abstract cognitive system.

The Theory of Localist Representation and of a Purely Abstract Cognitive System: The Evidence from Cortical Columns, Category Cells, and Multisensory Neurons

PubMed Central

Roy, Asim

2017-01-01

The debate about representation in the brain and the nature of the cognitive system has been going on for decades now. This paper examines the neurophysiological evidence, primarily from single cell recordings, to get a better perspective on both the issues. After an initial review of some basic concepts, the paper reviews the data from single cell recordings – in cortical columns and of category-selective and multisensory neurons. In neuroscience, columns in the neocortex (cortical columns) are understood to be a basic functional/computational unit. The paper reviews the fundamental discoveries about the columnar organization and finds that it reveals a massively parallel search mechanism. This columnar organization could be the most extensive neurophysiological evidence for the widespread use of localist representation in the brain. The paper also reviews studies of category-selective cells. The evidence for category-selective cells reveals that localist representation is also used to encode complex abstract concepts at the highest levels of processing in the brain. A third major issue is the nature of the cognitive system in the brain and whether there is a form that is purely abstract and encoded by single cells. To provide evidence for a single-cell based purely abstract cognitive system, the paper reviews some of the findings related to multisensory cells. It appears that there is widespread usage of multisensory cells in the brain in the same areas where sensory processing takes place. Plus there is evidence for abstract modality invariant cells at higher levels of cortical processing. Overall, that reveals the existence of a purely abstract cognitive system in the brain. The paper also argues that since there is no evidence for dense distributed representation and since sparse representation is actually used to encode memories, there is actually no evidence for distributed representation in the brain. Overall, it appears that, at an abstract level, the brain is a massively parallel, distributed computing system that is symbolic. The paper also explains how grounded cognition and other theories of the brain are fully compatible with localist representation and a purely abstract cognitive system. PMID:28261127

Isolation Mounting for Charge-Coupled Devices

NASA Technical Reports Server (NTRS)

Goss, W. C.; Salomon, P. M.

1985-01-01

CCD's suspended by wires under tension. Remote thermoelectric cooling of charge coupled device allows vibration isolating mounting of CCD assembly alone, without having to suspend entire mass and bulk of thermoelectric module. Mounting hardware simple and light. Developed for charge-coupled devices (CCD's) in infrared telescope support adaptable to sensors in variety of environments, e.g., sensors in nuclear reactors, engine exhausts and plasma chambers.

Test of CCD Precision Limits for Differential Photometry

NASA Technical Reports Server (NTRS)

Borucki, W. J.; Dunham, E. W.; Wei, M. Z.; Robinson, L. B.; Ford, C. H.; Granados, A. F.

1995-01-01

Results of tests to demonstrate the very high differential-photometric stability of CCD light sensors are presented. The measurements reported here demonstrate that in a controlled laboratory environment, a front-illuminated CCD can provide differential-photometric measurements with reproducible precision approaching one part in 105. Practical limitations to the precision of differential-photometric measurements with CCDs and implications for spaceborne applications are discussed.

Common Core of Data (CCD): School Years 1996-1997 through 2000-2001. [CD-ROM].

ERIC Educational Resources Information Center

National Center for Education Statistics (ED), Washington, DC.

The Common Core of Data (CCD) is NCES's primary database on elementary and secondary public education in the United States. CCD is a comprehensive, annual, national statistical database of all elementary and secondary schools and school districts, which contains data that are comparable across all states. The 50 states and the District of Columbia…

CTK-II & RTK: The CCD-cameras operated at the auxiliary telescopes of the University Observatory Jena

NASA Astrophysics Data System (ADS)

Mugrauer, M.

2016-03-01

The Cassegrain-Teleskop-Kamera (CTK-II) and the Refraktor-Teleskop-Kamera (RTK) are two CCD-imagers which are operated at the 25 cm Cassegrain and 20 cm refractor auxiliary telescopes of the University Observatory Jena. This article describes the main characteristics of these instruments. The properties of the CCD-detectors, the astrometry, the image quality, and the detection limits of both CCD-cameras, as well as some results of ongoing observing projects, carried out with these instruments, are presented. Based on observations obtained with telescopes of the University Observatory Jena, which is operated by the Astrophysical Institute of the Friedrich-Schiller-University.

CCD charge collection efficiency and the photon transfer technique

NASA Technical Reports Server (NTRS)

Janesick, J.; Klaasen, K.; Elliott, T.

1985-01-01

The charge-coupled device (CCD) has shown unprecendented performance as a photon detector in the areas of spectral response, charge transfer, and readout noise. Recent experience indicates, however, that the full potential for the CCD's charge collection efficiency (CCE) lies well beyond that which is realized in currently available devices. A definition of CCE performance is presented and a standard test tool (the photon transfer technique) for measuring and optimizing this important CCD parameter is introduced. CCE characteristics for different types of CCDs are compared; the primary limitations in achieving high CCE performance are discussed, and the prospects for future improvement are outlined.

Adolescent-onset alcohol abuse exacerbates the influence of childhood conduct disorder on late adolescent and early adult antisocial behaviour.

PubMed

Howard, Richard; Finn, Peter; Jose, Paul; Gallagher, Jennifer

2011-12-16

This study tested the hypothesis that adolescent-onset alcohol abuse (AOAA) would both mediate and moderate the effect of childhood conduct disorder on antisocial behaviour in late adolescence and early adulthood. A sample comprising 504 young men and women strategically recruited from the community were grouped using the criteria of the Diagnostic and Statistical Manual (DSM-IV, American Psychiatric Association. (1994). Diagnostic and statistical manual of mental disorders (4th ed.). Washington, DC: APA), as follows: neither childhood conduct disorder (CCD) nor alcohol abuse/dependence; CCD but no alcohol abuse or dependence; alcohol abuse/dependence but no CCD; both CCD and alcohol abuse/dependence. The outcome measure was the sum of positive responses to 55 interview items capturing a variety of antisocial behaviours engaged in since age 15. Severity of lifetime alcohol-related and CCD problems served as predictor variables in regression analysis. Antisocial behaviour problems were greatest in individuals with a history of co-occurring conduct disorder (CD) and alcohol abuse/dependence. While CCD was strongly predictive of adult antisocial behaviour, this effect was both mediated and moderated (exacerbated) by AOAA.

Combinations of coupled cluster, density functionals, and the random phase approximation for describing static and dynamic correlation, and van der Waals interactions

NASA Astrophysics Data System (ADS)

Garza, Alejandro J.; Bulik, Ireneusz W.; Alencar, Ana G. Sousa; Sun, Jianwei; Perdew, John P.; Scuseria, Gustavo E.

2016-04-01

Contrary to standard coupled cluster doubles (CCD) and Brueckner doubles (BD), singlet-paired analogues of CCD and BD (denoted here as CCD0 and BD0) do not break down when static correlation is present, but neglect substantial amounts of dynamic correlation. In fact, CCD0 and BD0 do not account for any contributions from multielectron excitations involving only same-spin electrons at all. We exploit this feature to add - without introducing double counting, self-interaction, or increase in cost - the missing correlation to these methods via meta-GGA (generalised gradient approximation) density functionals (Tao-Perdew-Staroverov-Scuseria and strongly constrained and appropriately normed). Furthermore, we improve upon these CCD0+DFT blends by invoking range separation: the short- and long-range correlations absent in CCD0/BD0 are evaluated with density functional theory and the direct random phase approximation, respectively. This corrects the description of long-range van der Waals forces. Comprehensive benchmarking shows that the combinations presented here are very accurate for weakly correlated systems, while also providing a reasonable description of strongly correlated problems without resorting to symmetry breaking.

Adolescent-onset alcohol abuse exacerbates the influence of childhood conduct disorder on late adolescent and early adult antisocial behaviour

PubMed Central

Howard, Richard; Finn, Peter; Jose, Paul; Gallagher, Jennifer

2012-01-01

This study tested the hypothesis that adolescent-onset alcohol abuse (AOAA) would both mediate and moderate the effect of childhood conduct disorder on antisocial behaviour in late adolescence and early adulthood. A sample comprising 504 young men and women strategically recruited from the community were grouped using the criteria of the Diagnostic and Statistical Manual (DSM-IV, American Psychiatric Association. (1994). Diagnostic and statistical manual of mental disorders (4th ed.). Washington, DC: APA), as follows: neither childhood conduct disorder (CCD) nor alcohol abuse/dependence; CCD but no alcohol abuse or dependence; alcohol abuse/dependence but no CCD; both CCD and alcohol abuse/dependence. The outcome measure was the sum of positive responses to 55 interview items capturing a variety of antisocial behaviours engaged in since age 15. Severity of lifetime alcohol-related and CCD problems served as predictor variables in regression analysis. Antisocial behaviour problems were greatest in individuals with a history of co-occurring conduct disorder (CD) and alcohol abuse/dependence. While CCD was strongly predictive of adult antisocial behaviour, this effect was both mediated and moderated (exacerbated) by AOAA. PMID:23459369

Systems approach to the design of the CCD sensors and camera electronics for the AIA and HMI instruments on solar dynamics observatory

NASA Astrophysics Data System (ADS)

Waltham, N.; Beardsley, S.; Clapp, M.; Lang, J.; Jerram, P.; Pool, P.; Auker, G.; Morris, D.; Duncan, D.

2017-11-01

Solar Dynamics Observatory (SDO) is imaging the Sun in many wavelengths near simultaneously and with a resolution ten times higher than the average high-definition television. In this paper we describe our innovative systems approach to the design of the CCD cameras for two of SDO's remote sensing instruments, the Atmospheric Imaging Assembly (AIA) and the Helioseismic and Magnetic Imager (HMI). Both instruments share use of a custom-designed 16 million pixel science-grade CCD and common camera readout electronics. A prime requirement was for the CCD to operate with significantly lower drive voltages than before, motivated by our wish to simplify the design of the camera readout electronics. Here, the challenge lies in the design of circuitry to drive the CCD's highly capacitive electrodes and to digitize its analogue video output signal with low noise and to high precision. The challenge is greatly exacerbated when forced to work with only fully space-qualified, radiation-tolerant components. We describe our systems approach to the design of the AIA and HMI CCD and camera electronics, and the engineering solutions that enabled us to comply with both mission and instrument science requirements.

Combination of Wavefunction and Density Functional Approximations for Describing Electronic Correlation

NASA Astrophysics Data System (ADS)

Garza, Alejandro J.

Perhaps the most important approximations to the electronic structure problem in quantum chemistry are those based on coupled cluster and density functional theories. Coupled cluster theory has been called the ``gold standard'' of quantum chemistry due to the high accuracy that it achieves for weakly correlated systems. Kohn-Sham density functionals based on semilocal approximations are, without a doubt, the most widely used methods in chemistry and material science because of their high accuracy/cost ratio. The root of the success of coupled cluster and density functionals is their ability to efficiently describe the dynamic part of the electron correlation. However, both traditional coupled cluster and density functional approximations may fail catastrophically when substantial static correlation is present. This severely limits the applicability of these methods to a plethora of important chemical and physical problems such as, e.g., the description of bond breaking, transition states, transition metal-, lanthanide- and actinide-containing compounds, and superconductivity. In an attempt to tackle this problem, nonstandard (single-reference) coupled cluster-based techniques that aim to describe static correlation have been recently developed: pair coupled cluster doubles (pCCD) and singlet-paired coupled cluster doubles (CCD0). The ability to describe static correlation in pCCD and CCD0 comes, however, at the expense of important amounts of dynamic correlation so that the high accuracy of standard coupled cluster becomes unattainable. Thus, the reliable and efficient description of static and dynamic correlation in a simultaneous manner remains an open problem for quantum chemistry and many-body theory in general. In this thesis, different ways to combine pCCD and CCD0 with density functionals in order to describe static and dynamic correlation simultaneously (and efficiently) are explored. The combination of wavefunction and density functional methods has a long history in quantum chemistry (practical implementations have appeared in the literature since the 1970s). However, this kind of techniques have not achieved widespread use due to problems such as double counting of correlation and the symmetry dilemma--the fact that wavefunction methods respect the symmetries of Hamiltonian, while modern functionals are designed to work with broken symmetry densities. Here, particular mathematical features of pCCD and CCD0 are exploited to avoid these problems in an efficient manner. The two resulting families of approximations, denoted as pCCD+DFT and CCD0+DFT, are shown to be able to describe static and dynamic correlation in standard benchmark calculations. Furthermore, it is also shown that CCD0+DFT lends itself to combination with correlation from the direct random phase approximation (dRPA). Inclusion of dRPA in the long-range via the technique of range-separation allows for the description of dispersion correlation, the remaining part of the correlation. Thus, when combined with the dRPA, CCD0+DFT can account for all three-types of electron correlation that are necessary to accurately describe molecular systems. Lastly, applications of CCD0+DFT to actinide chemistry are considered in this work. The accuracy of CCD0+DFT for predicting equilibrium geometries and vibrational frequencies of actinide molecules and ions is assessed and compared to that of well-established quantum chemical methods. For this purpose, the f0 actinyl series (UO2 2+, NpO 23+, PuO24+, the isoelectronic NUN, and Thorium (ThO, ThO2+) and Nobelium (NoO, NoO2) oxides are studied. It is shown that the CCD0+DFT description of these species agrees with available experimental data and is comparable with the results given by the highest-level calculations that are possible for such heavy compounds while being, at least, an order of magnitude lower in computational cost.

The prevalence and geographic distribution of complex co-occurring disorders: a population study.

PubMed

Somers, J M; Moniruzzaman, A; Rezansoff, S N; Brink, J; Russolillo, A

2016-06-01

A subset of people with co-occurring substance use and mental disorders require coordinated support from health, social welfare and justice agencies to achieve diversion from homelessness, criminal recidivism and further health and social harms. Integrated models of care are typically concentrated in large urban centres. The present study aimed to empirically measure the prevalence and distribution of complex co-occurring disorders (CCD) in a large geographic region that includes urban as well as rural and remote settings. Linked data were examined in a population of roughly 3.7 million adults. Inclusion criteria for the CCD subpopulation were: physician diagnosed substance use and mental disorders; psychiatric hospitalisation; shelter assistance; and criminal convictions. Prevalence per 100 000 was calculated in 91 small areas representing urban, rural and remote settings. 2202 individuals met our inclusion criteria for CCD. Participants had high rates of hospitalisation (8.2 admissions), criminal convictions (8.6 sentences) and social assistance payments (over $36 000 CDN) in the past 5 years. There was wide variability in the geographic distribution of people with CCD, with high prevalence rates in rural and remote settings. People with CCD are not restricted to areas with large populations or to urban settings. The highest per capita rates of CCD were observed in relatively remote locations, where mental health and substance use services are typically in limited supply. Empirically supported interventions must be adapted to meet the needs of people living outside of urban settings with high rates of CCD.

Control and management of congenital Chagas disease in Europe and other non-endemic countries: current policies and practices.

PubMed

Soriano-Arandes, Antoni; Angheben, Andrea; Serre-Delcor, Nuria; Treviño-Maruri, Begoña; Gómez I Prat, Jordi; Jackson, Yves

2016-05-01

Identifying pregnant women infected with Trypanosoma cruzi is one of the major challenges for preventing and controlling Chagas disease (CD) in non-endemic countries. The aim of this paper was to perform a policy evaluation of the current practices of congenital Chagas disease (CCD) control in non-endemic countries and to propose specific targets for enhanced interventions to tackle this emerging health problem outside the endemic areas of Latin America. We conducted a mixed method review of CCD policy strategies by searching the literature in the PubMed, Google Scholar and the World Health Organization (WHO) databases using the key terms 'CCD', 'paediatric Chagas disease' and 'non-endemic countries'; as free text and combined as one phrase to increase the search sensitivity. Reviews, recommendations, guidelines and control/surveillance programme reports were included. Of 427 CCD papers identified in non-endemic countries, 44 matched the inclusion. Although local programmes were launched in different countries with large numbers of Latin American immigrants, there were considerable disparities in terms of the programmes' distribution, delivery, integration and appropriated CCD control strategies. Moreover, Catalonia, Spain is the only region/country with an established systematic monitoring of CCD in pregnant women from Latin American countries. Given the worldwide dissemination of CD, the nature of its vertical transmission, and the gaps of the current strategies in non-endemic countries, there is an urgent need to standardise, expand and reinforce the control measures against CCD transmission. © 2016 John Wiley & Sons Ltd.

Development and use of an L3CCD high-cadence imaging system for Optical Astronomy

NASA Astrophysics Data System (ADS)

Sheehan, Brendan J.; Butler, Raymond F.

2008-02-01

A high cadence imaging system, based on a Low Light Level CCD (L3CCD) camera, has been developed for photometric and polarimetric applications. The camera system is an iXon DV-887 from Andor Technology, which uses a CCD97 L3CCD detector from E2V technologies. This is a back illuminated device, giving it an extended blue response, and has an active area of 512×512 pixels. The camera system allows frame-rates ranging from 30 fps (full frame) to 425 fps (windowed & binned frame). We outline the system design, concentrating on the calibration and control of the L3CCD camera. The L3CCD detector can be either triggered directly by a GPS timeserver/frequency generator or be internally triggered. A central PC remotely controls the camera computer system and timeserver. The data is saved as standard `FITS' files. The large data loads associated with high frame rates, leads to issues with gathering and storing the data effectively. To overcome such problems, a specific data management approach is used, and a Python/PYRAF data reduction pipeline was written for the Linux environment. This uses calibration data collected either on-site, or from lab based measurements, and enables a fast and reliable method for reducing images. To date, the system has been used twice on the 1.5 m Cassini Telescope in Loiano (Italy) we present the reduction methods and observations made.

Stress-Strain Properties of SIFCON in Uniaxial Compression and Tension

DTIC Science & Technology

1988-08-01

direction act as contacting beams whereas fibers aligned parallel to the loading direction act as individual columns . The combination of fiber-to-fiber...applicable to the study of SIFCON. These include such topics as the influence of strain rate on composite behavior, cyclic loading response, fiber-to-matrix...the specimen are shown in Figure 17. The grips consisted of self-clamping steel plates and a universal joint connection to the loading machine which

High-resolution, high-throughput imaging with a multibeam scanning electron microscope.

PubMed

Eberle, A L; Mikula, S; Schalek, R; Lichtman, J; Knothe Tate, M L; Zeidler, D

2015-08-01

Electron-electron interactions and detector bandwidth limit the maximal imaging speed of single-beam scanning electron microscopes. We use multiple electron beams in a single column and detect secondary electrons in parallel to increase the imaging speed by close to two orders of magnitude and demonstrate imaging for a variety of samples ranging from biological brain tissue to semiconductor wafers. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

The reliability of cytoplasmic CD3 and CD22 antigen expression in the immunodiagnosis of acute leukemia: a study of 500 cases.

PubMed

Janossy, G; Coustan-Smith, E; Campana, D

1989-03-01

Current views about the origin of acute lymphoid leukemia (ALL) emphasize the importance of maturation arrest at a precursor cell level. Recently, the CD22 antigen has been identified in the cytoplasm of normal bone marrow-borne immature B lineage cells, while the CD3 antigen (epsilon chain) has been detected within normal immature thymic blasts. In the first part our study performed on 100 cases of known acute leukemias, the expression of such cytoplasmic molecules, referred to as cCD22 and cCD3, was analyzed together with their appearance in the leukemic cells' membrane (mCD22 and mCD3). The presence of cCD22 in B-lineage ALL and that of cCD3 in T-ALL has indeed fully confirmed the diagnosis reached by other markers, and mCD22 and mCD3 were expressed on only a few cases of B- and T-lineage ALL, also revealing a degree of developmental asynchrony within leukemic blasts. In the subsequent analysis both cCD22 and cCD3 have been included in a standard panel of diagnostic reagents applied on 500 consecutive cases of acute leukemia. Here the aim was to analyze both the diagnostic precision of individual markers and the heterogeneity of various leukemic types in terms of the expression of membrane and intracellular antigens and their cytochemical features (Sudan Black B and esterases). It has been found that cCD22 and cCD3 are exquisitely specific for B-precursor ALL (TdT+, CD19+) and T-ALL (TdT+, CD7+), respectively, while both markers are absent in acute myeloblastic leukemia (AML) and acute myelomonocytic and monocytic leukemia (AMML/AMoL). These observations contrast the findings which demonstrate that 31% of cases among nonlymphoid acute leukemia (including AML and AMML) express CD7 and/or TdT. The study of myeloid antigens detected by CD13, CD33, and CD14 is also informative and complementary, both in diagnosing and subdividing the AML and AMML/AMoL groups. The peculiar main observation of this study is that only with the combined use of these markers in a microplate assay for membrane antigens, followed by double staining for intracellular antigens such as terminal deoxynucleotidyl transferase, cCD3, cCD22, c mu heavy chain, and T cell receptor beta, it is possible to safely establish the lineage affiliation and subgrouping of virtually all acute leukemias. Among these cases are those with aberrant combinations of markers, including 14% of B-lineage ALL (cCD22+,CD19+,TdT+) and a single case T-ALL (cCD3+,CD7+,TdT+), which exhibit CD13 and/or CD33 antigens, cases with mixtures of ALL and AML blasts, and 1.2% of acute leukemias which lack lineage affiliation and can be regarded as acute undifferentiated leukemia.

Development of a 3D parallel mechanism robot arm with three vertical-axial pneumatic actuators combined with a stereo vision system.

PubMed

Chiang, Mao-Hsiung; Lin, Hao-Ting

2011-01-01

This study aimed to develop a novel 3D parallel mechanism robot driven by three vertical-axial pneumatic actuators with a stereo vision system for path tracking control. The mechanical system and the control system are the primary novel parts for developing a 3D parallel mechanism robot. In the mechanical system, a 3D parallel mechanism robot contains three serial chains, a fixed base, a movable platform and a pneumatic servo system. The parallel mechanism are designed and analyzed first for realizing a 3D motion in the X-Y-Z coordinate system of the robot's end-effector. The inverse kinematics and the forward kinematics of the parallel mechanism robot are investigated by using the Denavit-Hartenberg notation (D-H notation) coordinate system. The pneumatic actuators in the three vertical motion axes are modeled. In the control system, the Fourier series-based adaptive sliding-mode controller with H(∞) tracking performance is used to design the path tracking controllers of the three vertical servo pneumatic actuators for realizing 3D path tracking control of the end-effector. Three optical linear scales are used to measure the position of the three pneumatic actuators. The 3D position of the end-effector is then calculated from the measuring position of the three pneumatic actuators by means of the kinematics. However, the calculated 3D position of the end-effector cannot consider the manufacturing and assembly tolerance of the joints and the parallel mechanism so that errors between the actual position and the calculated 3D position of the end-effector exist. In order to improve this situation, sensor collaboration is developed in this paper. A stereo vision system is used to collaborate with the three position sensors of the pneumatic actuators. The stereo vision system combining two CCD serves to measure the actual 3D position of the end-effector and calibrate the error between the actual and the calculated 3D position of the end-effector. Furthermore, to verify the feasibility of the proposed parallel mechanism robot driven by three vertical pneumatic servo actuators, a full-scale test rig of the proposed parallel mechanism pneumatic robot is set up. Thus, simulations and experiments for different complex 3D motion profiles of the robot end-effector can be successfully achieved. The desired, the actual and the calculated 3D position of the end-effector can be compared in the complex 3D motion control.

Development of a 3D Parallel Mechanism Robot Arm with Three Vertical-Axial Pneumatic Actuators Combined with a Stereo Vision System

PubMed Central

Chiang, Mao-Hsiung; Lin, Hao-Ting

2011-01-01

This study aimed to develop a novel 3D parallel mechanism robot driven by three vertical-axial pneumatic actuators with a stereo vision system for path tracking control. The mechanical system and the control system are the primary novel parts for developing a 3D parallel mechanism robot. In the mechanical system, a 3D parallel mechanism robot contains three serial chains, a fixed base, a movable platform and a pneumatic servo system. The parallel mechanism are designed and analyzed first for realizing a 3D motion in the X-Y-Z coordinate system of the robot’s end-effector. The inverse kinematics and the forward kinematics of the parallel mechanism robot are investigated by using the Denavit-Hartenberg notation (D-H notation) coordinate system. The pneumatic actuators in the three vertical motion axes are modeled. In the control system, the Fourier series-based adaptive sliding-mode controller with H∞ tracking performance is used to design the path tracking controllers of the three vertical servo pneumatic actuators for realizing 3D path tracking control of the end-effector. Three optical linear scales are used to measure the position of the three pneumatic actuators. The 3D position of the end-effector is then calculated from the measuring position of the three pneumatic actuators by means of the kinematics. However, the calculated 3D position of the end-effector cannot consider the manufacturing and assembly tolerance of the joints and the parallel mechanism so that errors between the actual position and the calculated 3D position of the end-effector exist. In order to improve this situation, sensor collaboration is developed in this paper. A stereo vision system is used to collaborate with the three position sensors of the pneumatic actuators. The stereo vision system combining two CCD serves to measure the actual 3D position of the end-effector and calibrate the error between the actual and the calculated 3D position of the end-effector. Furthermore, to verify the feasibility of the proposed parallel mechanism robot driven by three vertical pneumatic servo actuators, a full-scale test rig of the proposed parallel mechanism pneumatic robot is set up. Thus, simulations and experiments for different complex 3D motion profiles of the robot end-effector can be successfully achieved. The desired, the actual and the calculated 3D position of the end-effector can be compared in the complex 3D motion control. PMID:22247676

Carbofuran biodegradation in brackish groundwater and its effect on the hydraulic properties of the porous medium

NASA Astrophysics Data System (ADS)

Amiaz, Yanai; Ronen, Zeev; Adar, Eilon; Weisbrod, Noam

2015-04-01

A chalk fractured aquitard beneath an industrial site is subjected to intense contamination due to percolation of contaminants from the different facilities operating at the site. In order to reduce further contamination, draining trenches were excavated and filled with coarse gravel (3-4 cm in diameter) forming a porous medium, to which the contaminated groundwater discharges from the fractures surrounding the trenches. This research is aimed at establishing a biodegrading process of high efficiency and performance within the draining trenches. The research includes both field and laboratory experiments. An experimental setup of five columns (50 cm length and 4.5 cm in diameter) was constructed under highly controlled conditions. Over the course of the experiments, the columns were filled with different particle sizes and placed in a temperature controlled chamber. Filtered groundwater (0.2 µm) from the site groundwater, enriched by a model contaminant carbofuran (CRF), was injected to the columns; as two of the columns were inoculated by CRF degrading microorganisms native in the site's groundwater, two columns were inoculated by CRF degrading bacteria from the external environment, and one column was used as a control. During the experiment, measurements were taken from different locations along each column. These include: (a) CRF concentration and (b) hydraulic pressure and solution viscosity (in order to obtain the changes in permeability). A tracer test using uranine was carried out in parallel, in order to obtain the changes in hydraulic parameters. Correlating CRF concentration variations to changes of hydraulic parameters enable the deduction due to the effect that biological activity (under different temperature regimes) has on the hydraulic properties of the porous medium and its effect on the process of contaminant groundwater bodies' remediation. Preliminary results suggest that although biodegradation occurs, microbial activity has minor effect on the hydraulic properties of the porous medium under the explored conditions.

Test of CCD Precision Limits for Differential Photometry

NASA Technical Reports Server (NTRS)

Robinson, L. B.; Wei, M. Z.; Borucki, W. J.; Dunham, E. W.; Ford, C. H.; Granados, A. F.

1995-01-01

Results of tests to demonstrate the very high differential-photometric stability of CCD light sensors are presented. The measurements reported here demonstrate that in a controlled laboratory environment, a front-illuminated CCD can provide differential-photometric measurements with reproducible precision approaching one part in 10(exp 5). Practical limitations to the precision of differential-photometric measurements with CCDs and implications for spaceborne applications are discussed.

Dynamically reconfigurable photovoltaic system

DOEpatents

Okandan, Murat; Nielson, Gregory N.

2016-05-31

A PV system composed of sub-arrays, each having a group of PV cells that are electrically connected to each other. A power management circuit for each sub-array has a communications interface and serves to connect or disconnect the sub-array to a programmable power grid. The power grid has bus rows and bus columns. A bus management circuit is positioned at a respective junction of a bus column and a bus row and is programmable through its communication interface to connect or disconnect a power path in the grid. As a result, selected sub-arrays are connected by selected power paths to be in parallel so as to produce a low system voltage, and, alternately in series so as to produce a high system voltage that is greater than the low voltage by at least a factor of ten.

Dynamically reconfigurable photovoltaic system

DOEpatents

Okandan, Murat; Nielson, Gregory N.

2016-12-27

A PV system composed of sub-arrays, each having a group of PV cells that are electrically connected to each other. A power management circuit for each sub-array has a communications interface and serves to connect or disconnect the sub-array to a programmable power grid. The power grid has bus rows and bus columns. A bus management circuit is positioned at a respective junction of a bus column and a bus row and is programmable through its communication interface to connect or disconnect a power path in the grid. As a result, selected sub-arrays are connected by selected power paths to be in parallel so as to produce a low system voltage, and, alternately in series so as to produce a high system voltage that is greater than the low voltage by at least a factor of ten.

Flat-panel detector, CCD cameras, and electron-beam-tube-based video for use in portal imaging

NASA Astrophysics Data System (ADS)

Roehrig, Hans; Tang, Chuankun; Cheng, Chee-Way; Dallas, William J.

1998-07-01

This paper provides a comparison of some imaging parameters of four portal imaging systems at 6 MV: a flat panel detector, two CCD cameras and an electron beam tube based video camera. Measurements were made of signal and noise and consequently of signal-to-noise per pixel as a function of the exposure. All systems have a linear response with respect to exposure, and with the exception of the electron beam tube based video camera, the noise is proportional to the square-root of the exposure, indicating photon-noise limitation. The flat-panel detector has a signal-to-noise ratio, which is higher than that observed with both CCD-Cameras or with the electron beam tube based video camera. This is expected because most portal imaging systems using optical coupling with a lens exhibit severe quantum-sinks. The measurements of signal-and noise were complemented by images of a Las Vegas-type aluminum contrast detail phantom, located at the ISO-Center. These images were generated at an exposure of 1 MU. The flat-panel detector permits detection of Aluminum holes of 1.2 mm diameter and 1.6 mm depth, indicating the best signal-to-noise ratio. The CCD-cameras rank second and third in signal-to- noise ratio, permitting detection of Aluminum-holes of 1.2 mm diameter and 2.2 mm depth (CCD_1) and of 1.2 mm diameter and 3.2 mm depth (CCD_2) respectively, while the electron beam tube based video camera permits detection of only a hole of 1.2 mm diameter and 4.6 mm depth. Rank Order Filtering was applied to the raw images from the CCD-based systems in order to remove the direct hits. These are camera responses to scattered x-ray photons which interact directly with the CCD of the CCD-Camera and generate 'Salt and Pepper type noise,' which interferes severely with attempts to determine accurate estimates of the image noise. The paper also presents data on the metal-phosphor's photon gain (the number of light-photons per interacting x-ray photon).

Measuring the effective pixel positions for the HARPS3 CCD

NASA Astrophysics Data System (ADS)

Hall, Richard D.; Thompson, Samantha; Queloz, Didier

2016-07-01

We present preliminary results from an experiment designed to measure the effective pixel positions of a CCD to sub-pixel precision. This technique will be used to characterise the 4k x 4k CCD destined for the HARPS-3 spectrograph. The principle of coherent beam interference is used to create intensity fringes along one axis of the CCD. By sweeping the physical parameters of the experiment, the geometry of the fringes can be altered which is used to probe the pixel structure. We also present the limitations of the current experimental set-up and suggest what will be implemented in the future to vastly improve the precision of the measurements.

Vacuum compatible miniature CCD camera head

DOEpatents

Conder, Alan D.

2000-01-01

A charge-coupled device (CCD) camera head which can replace film for digital imaging of visible light, ultraviolet radiation, and soft to penetrating x-rays, such as within a target chamber where laser produced plasmas are studied. The camera head is small, capable of operating both in and out of a vacuum environment, and is versatile. The CCD camera head uses PC boards with an internal heat sink connected to the chassis for heat dissipation, which allows for close(0.04" for example) stacking of the PC boards. Integration of this CCD camera head into existing instrumentation provides a substantial enhancement of diagnostic capabilities for studying high energy density plasmas, for a variety of military industrial, and medical imaging applications.

Measurements of 161 Double Stars With a High-Speed CCD: The Winter/Spring 2017 Observing Program at Brilliant Sky Observatory, Part 2

NASA Astrophysics Data System (ADS)

Harshaw, Richard

2018-04-01

In the winter and spring of 2017, an aggressive observing program of measuring close double stars with speckle interferometry and CCD imaging was undertaken at Brilliant Sky Observatory, my observing site in Cave Creek, Arizona. A total of 596 stars were observed, 8 of which were rejected for various reasons, leaving 588 pairs. Of these, 427 were observed and measured with speckle interferometry, while the remaining 161 were measured with a CCD. This paper reports the results of the observations of the 161 CCD cases. A separate paper in this issue will report the speckle measurements of the 427 other pairs.

Modal and thermal analysis of Les Arches unstable rock column (Vercors massif, French Alps)

NASA Astrophysics Data System (ADS)

Bottelin, P.; Lévy, C.; Baillet, L.; Jongmans, D.; Guéguen, P.

2013-08-01

A potentially unstable limestone column (˜1000 m3, Vercors, French Alps) delineated by an open rear fracture was continuously instrumented with two three-component seismic sensors from mid-May 2009 to mid-October 2011. Spectral analysis of seismic noise allowed several resonance frequencies to be determined, ranging from 6 to 21 Hz. The frequency domain decomposition (FDD) technique was applied to the ambient vibrations recorded on the top of the rock column. Three vibration modes were identified at 6, 7.5 and 9 Hz, describing the upper part of corresponding modal shapes. Finite element numerical modelling of the column dynamic response confirmed that the first two modes are bending modes perpendicular and parallel to the fracture, respectively, while the third one corresponds to torsion. Seismic noise monitoring also pointed out that resonance frequencies fluctuate with time, under thermomechanical control. For seasonal cycles, changes in frequency are due to the variations of the bulk elastic properties with temperature. At daily scale, increase in fundamental frequency with temperature has been interpreted as resulting from the rock expansion inducing a closure of the rear fracture rock bridges, hence stiffening the contact between the column and the rock mass. Conversely, the rock contraction induces a fracture opening and a decrease in resonance frequency. In winter, when the temperature drops below 0 °C, a dramatic increase in fundamental frequency is observed from 6 Hz to more than 25 Hz, resulting from ice formation in the fracture. During spring, the resonance frequency gradually diminishes with ice melting to reach the value measured before winter.

Characterization of viral siRNA populations in honey bee colony collapse disorder.

PubMed

Chejanovsky, Nor; Ophir, Ron; Schwager, Michal Sharabi; Slabezki, Yossi; Grossman, Smadar; Cox-Foster, Diana

2014-04-01

Colony Collapse Disorder (CCD), a special case of collapse of honey bee colonies, has resulted in significant losses for beekeepers. CCD-colonies show abundance of pathogens which suggests that they have a weakened immune system. Since honey bee viruses are major players in colony collapse and given the important role of viral RNA interference (RNAi) in combating viral infections we investigated if CCD-colonies elicit an RNAi response. Deep-sequencing analysis of samples from CCD-colonies from US and Israel revealed abundant small interfering RNAs (siRNA) of 21-22 nucleotides perfectly matching the Israeli acute paralysis virus (IAPV), Kashmir virus and Deformed wing virus genomes. Israeli colonies showed high titers of IAPV and a conserved RNAi-pattern of matching the viral genome. That was also observed in sample analysis from colonies experimentally infected with IAPV. Our results suggest that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses. Copyright © 2014 Elsevier Inc. All rights reserved.

Enabling Joint Commission Medication Reconciliation Objectives with the HL7 / ASTM Continuity of Care Document Standard

PubMed Central

Dolin, Robert H.; Giannone, Gay; Schadow, Gunther

2007-01-01

We sought to determine how well the HL7 / ASTM Continuity of Care Document (CCD) standard supports the requirements underlying the Joint Commission medication reconciliation recommendations. In particular, the Joint Commission emphasizes that transition points in the continuum of care are vulnerable to communication breakdowns, and that these breakdowns are a common source of medication errors. These transition points are the focus of communication standards, suggesting that CCD can support and enable medication related patient safety initiatives. Data elements needed to support the Joint Commission recommendations were identified and mapped to CCD, and a detailed clinical scenario was constructed. The mapping identified minor gaps, and identified fields present in CCD not specifically identified by Joint Commission, but useful nonetheless when managing medications across transitions of care, suggesting that a closer collaboration between the Joint Commission and standards organizations will be mutually beneficial. The nationally recognized CCD specification provides a standards-based solution for enabling Joint Commission medication reconciliation objectives. PMID:18693823

Enabling joint commission medication reconciliation objectives with the HL7 / ASTM Continuity of Care Document standard.

PubMed

Dolin, Robert H; Giannone, Gay; Schadow, Gunther

2007-10-11

We sought to determine how well the HL7/ASTM Continuity of Care Document (CCD) standard supports the requirements underlying the Joint Commission medication reconciliation recommendations. In particular, the Joint Commission emphasizes that transition points in the continuum of care are vulnerable to communication breakdowns, and that these breakdowns are a common source of medication errors. These transition points are the focus of communication standards, suggesting that CCD can support and enable medication related patient safety initiatives. Data elements needed to support the Joint Commission recommendations were identified and mapped to CCD, and a detailed clinical scenario was constructed. The mapping identified minor gaps, and identified fields present in CCD not specifically identified by Joint Commission, but useful nonetheless when managing medications across transitions of care, suggesting that a closer collaboration between the Joint Commission and standards organizations will be mutually beneficial. The nationally recognized CCD specification provides a standards-based solution for enabling Joint Commission medication reconciliation objectives.

The Speckle Toolbox: A Powerful Data Reduction Tool for CCD Astrometry

NASA Astrophysics Data System (ADS)

Harshaw, Richard; Rowe, David; Genet, Russell

2017-01-01

Recent advances in high-speed low-noise CCD and CMOS cameras, coupled with breakthroughs in data reduction software that runs on desktop PCs, has opened the domain of speckle interferometry and high-accuracy CCD measurements of double stars to amateurs, allowing them to do useful science of high quality. This paper describes how to use a speckle interferometry reduction program, the Speckle Tool Box (STB), to achieve this level of result. For over a year the author (Harshaw) has been using STB (and its predecessor, Plate Solve 3) to obtain measurements of double stars based on CCD camera technology for pairs that are either too wide (the stars not sharing the same isoplanatic patch, roughly 5 arc-seconds in diameter) or too faint to image in the coherence time required for speckle (usually under 40ms). This same approach - using speckle reduction software to measure CCD pairs with greater accuracy than possible with lucky imaging - has been used, it turns out, for several years by the U. S. Naval Observatory.

Single-Electron and Single-Photon Sensitivity with a Silicon Skipper CCD

DOE PAGES

Tiffenberg, Javier; Sofo-Haro, Miguel; Drlica-Wagner, Alex; ...

2017-09-26

Here, we have developed ultralow-noise electronics in combination with repetitive, nondestructive readout of a thick, fully depleted charge-coupled device (CCD) to achieve an unprecedented noise level of 0.068 e - rms/pixel. This is the first time that discrete subelectron readout noise has been achieved reproducible over millions of pixels on a stable, large-area detector. This enables the contemporaneous, discrete, and quantized measurement of charge in pixels, irrespective of whether they contain zero electrons or thousands of electrons. Thus, the resulting CCD detector is an ultra-sensitive calorimeter. It is also capable of counting single photons in the optical and near-infrared regime.more » Implementing this innovative non-destructive readout system has a negligible impact on CCD design and fabrication, and there are nearly immediate scientific applications. As a particle detector, this CCD will have unprecedented sensitivity to low-mass dark matter particles and coherent neutrino-nucleus scattering, while future astronomical applications may include direct imaging and spectroscopy of exoplanets.« less

Perceptions of predisposing, enabling, and reinforcing factors influencing the use of a continuity of care document in special needs PLWH.

PubMed

Odlum, Michelle; Gordon, Peter; Camhi, Eli; Schnall, Rebecca; Teixeira, Paul; Bakken, Suzanne

2012-11-01

The provision of personal health information through electronic personal health management tools (EPHMT) has the potential to improve health outcomes. However, little is known about factors that affect EPHMT use in special needs people living with HIV/AIDS (PLWH). The purpose of this study was to describe PLWH perceptions of predisposing, enabling, and reinforcing factors affecting use of one type of EPHMT, a continuity of care document (CCD). Data were collected through focus groups and qualitative interviews with PLWH (n=35), health care providers (N=19) and care site managers (N=5). All participant types identified predisposing barriers to CCD use including literacy, privacy, and technology access and enabling factors to promote CCD use including user support and password simplicity. People living with HIV/AIDS also identified reinforcing factors for recurring CCD use including emergent situations and reducing test redundancy. Results inform methods to promote use of the CCD and other EPHMT by special needs PLWH.

CCD Measurements of Double and Multiple Stars at NAO Rozhen and ASV in 2015

NASA Astrophysics Data System (ADS)

Cvetković, Z.; Pavlović, R.; Boeva, S.

2017-04-01

Results of CCD observations of 154 double or multiple stars, made with the 2 m telescope of the Bulgarian National Astronomical Observatory at Rozhen over five nights in 2015, are presented. This is the ninth series of measurements of CCD frames obtained at Rozhen. We also present results of CCD observations of 323 double or multiple stars made with the 0.6 m telescope of the Serbian Astronomical Station on the mountain of Vidojevica over 23 nights in 2015. This is the fourth series of measurements of CCD frames obtained at this station. This paper contains the results for the position angle and angular separation for 801 pairs and residuals for 127 pairs with published orbital elements or linear solutions. The angular separations are in the range from 1.″52 to 201.″56, with a median angular separation of 8.″26. We also present eight pairs that are measured for the first time and linear elements for five pairs.

Concordia CCD - A Geoscope station in continental Antarctica

NASA Astrophysics Data System (ADS)

Maggi, A.; Lévêque, J.; Thoré, J.; Bes de Berc, M.; Bernard, A.; Danesi, S.; Morelli, A.; Delladio, A.; Sorrentino, D.; Stutzmann, E.; Geoscope Team

2010-12-01

Concordia (Dome C, Antarctica) has had a permanent seismic station since 2005. It is run by EOST and INGV in collaboration with the French and Italian polar institutes (IPEV and PNRA). It is installed in an ice-vault, at 12m depth, distant 1km from the permanent scientific base at Concordia. The temperature in the vault is a constant -55°C. The data quality at the station has improved continuously since its installation. In 2007, the station was declared at ISC as an open station with station code CCD (ConCorDia), with data available upon request. It is only the second permanent station in the Antarctic continent, after South Pole. In 2010, CCD was included in the Geoscope network. Data from CCD starting in 2007 are now freely available from the Geoscope Data Center and IRIS. We present an analysis of the data quality at CCD, and describe the technical difficulties of operating an observatory-quality seismic station in the extreme environmental conditons present in continental Antarctica.

Single-Electron and Single-Photon Sensitivity with a Silicon Skipper CCD

NASA Astrophysics Data System (ADS)

Tiffenberg, Javier; Sofo-Haro, Miguel; Drlica-Wagner, Alex; Essig, Rouven; Guardincerri, Yann; Holland, Steve; Volansky, Tomer; Yu, Tien-Tien

2017-09-01

We have developed ultralow-noise electronics in combination with repetitive, nondestructive readout of a thick, fully depleted charge-coupled device (CCD) to achieve an unprecedented noise level of 0.068 e- rms /pixel . This is the first time that discrete subelectron readout noise has been achieved reproducible over millions of pixels on a stable, large-area detector. This enables the contemporaneous, discrete, and quantized measurement of charge in pixels, irrespective of whether they contain zero electrons or thousands of electrons. Thus, the resulting CCD detector is an ultra-sensitive calorimeter. It is also capable of counting single photons in the optical and near-infrared regime. Implementing this innovative non-destructive readout system has a negligible impact on CCD design and fabrication, and there are nearly immediate scientific applications. As a particle detector, this CCD will have unprecedented sensitivity to low-mass dark matter particles and coherent neutrino-nucleus scattering, while future astronomical applications may include direct imaging and spectroscopy of exoplanets.

Single-Electron and Single-Photon Sensitivity with a Silicon Skipper CCD.

PubMed

Tiffenberg, Javier; Sofo-Haro, Miguel; Drlica-Wagner, Alex; Essig, Rouven; Guardincerri, Yann; Holland, Steve; Volansky, Tomer; Yu, Tien-Tien

2017-09-29

We have developed ultralow-noise electronics in combination with repetitive, nondestructive readout of a thick, fully depleted charge-coupled device (CCD) to achieve an unprecedented noise level of 0.068  e^{-} rms/pixel. This is the first time that discrete subelectron readout noise has been achieved reproducible over millions of pixels on a stable, large-area detector. This enables the contemporaneous, discrete, and quantized measurement of charge in pixels, irrespective of whether they contain zero electrons or thousands of electrons. Thus, the resulting CCD detector is an ultra-sensitive calorimeter. It is also capable of counting single photons in the optical and near-infrared regime. Implementing this innovative non-destructive readout system has a negligible impact on CCD design and fabrication, and there are nearly immediate scientific applications. As a particle detector, this CCD will have unprecedented sensitivity to low-mass dark matter particles and coherent neutrino-nucleus scattering, while future astronomical applications may include direct imaging and spectroscopy of exoplanets.

VizieR Online Data Catalog: IC 361 Vilnius photometry (Zdanavicius+, 2010)

NASA Astrophysics Data System (ADS)

Zdanavicius, J.; Bartasiute, S.; Boyle, R. P.; Vrba, F. J.; Zdanavicius, K.

2015-03-01

CCD observations in seven filters U,P,X,Y,Z,V,S of the Vilnius system plus the filter I of the Cousins system were carried out in December of 1999 with a 2K CCD camera on the 1m telescope of the USNO Flagstaff Station (Arizona), which gives a field of the diameter of 20'. Repeated observations in the Vilnius filters were done with the same telescope and a new 2Kx2K CCD camera in March of 2009. During the latter run we have obtained well-calibrated CCD data only for filters Y, Z, V, S, since observations through the remaining three filters on the succeeding night were curtailed by cirrus clouds. Additional frames in the Vilnius filters U,Y,V were taken for the central part of the field (12'x12') in December of 2008 with a 4K CCD camera on the 1.8m Vatican Advanced Technology Telescope (VATT) on Mt. Graham (Arizona). (1 data file).

Fast Electrically Driven Capillary Rise Using Overdrive Voltage.

PubMed

Hong, Sung Jin; Hong, Jiwoo; Seo, Hee Won; Lee, Sang Joon; Chung, Sang Kug

2015-12-29

Enhancement of response speed (or reduction of response time) is crucial for the commercialization of devices based on electrowetting (EW), such as liquid lenses and reflective displays, and presents one of the main challenges in EW research studies. We demonstrate here that an overdrive EW actuation gives rise to a faster rise of a liquid column between parallel electrodes, compared to a DC EW actuation. Here, DC actuation is actually a simple applied step function, and overdrive is an applied step followed by reduction to a lower voltage. Transient behaviors and response time (i.e., the time required to reach the equilibrium height) of the rising liquid column are explored under different DC and overdrive EW actuations. When the liquid column rises up to a target height by means of an overdrive EW, the response time is reduced to as low as 1/6 of the response time using DC EW. We develop a theoretical model to simulate the EW-driven capillary rise by combining the kinetic equation of capillary flow (i.e., Lucas-Washburn equation) and the dynamic contact angle model considering contact line friction, contact angle hysteresis, contact angle saturation, and the EW effect. This theoretical model accurately predicts the outcome to within a ± 5% error in regard to the rising behaviors of the liquid column with a low viscosity, under both DC EW and overdrive actuation conditions, except for the early stage (

Colony collapse disorder in Europe.

PubMed

Dainat, Benjamin; Vanengelsdorp, Dennis; Neumann, Peter

2012-02-01

Colony collapse disorder (CCD) is a condition of honey bees, which has contributed in part to the recent major losses of honey bee colonies in the USA. Here we report the first CCD case from outside of the USA. We suggest that more standardization is needed for the case definition to diagnose CCD and to compare data on a global scale. © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.

Performance of the STIS CCD Dark Rate Temperature Correction

NASA Astrophysics Data System (ADS)

Branton, Doug; STScI STIS Team

2018-06-01

Since July 2001, the Space Telescope Imaging Spectrograph (STIS) onboard Hubble has operated on its Side-2 electronics due to a failure in the primary Side-1 electronics. While nearly identical, Side-2 lacks a functioning temperature sensor for the CCD, introducing a variability in the CCD operating temperature. Previous analysis utilized the CCD housing temperature telemetry to characterize the relationship between the housing temperature and the dark rate. It was found that a first-order 7%/°C uniform dark correction demonstrated a considerable improvement in the quality of dark subtraction on Side-2 era CCD data, and that value has been used on all Side-2 CCD darks since. In this report, we show how this temperature correction has performed historically. We compare the current 7%/°C value against the ideal first-order correction at a given time (which can vary between ~6%/°C and ~10%/°C) as well as against a more complex second-order correction that applies a unique slope to each pixel as a function of dark rate and time. At worst, the current correction has performed ~1% worse than the second-order correction. Additionally, we present initial evidence suggesting that the variability in pixel temperature-sensitivity is significant enough to warrant a temperature correction that considers pixels individually rather than correcting them uniformly.

Cross-reacting carbohydrate determinants and hymenoptera venom allergy.

PubMed

Brehler, Randolf; Grundmann, Sonja; Stöcker, Benedikt

2013-08-01

Insect venom allergy is an important cause of anaphylaxis. Venom immunotherapy assume the clear identification of the culprit insect, but this is impeded by Immunoglobulin E (IgE) antibodies to cross reactive carbohydrate determinant (CCD) epitopes of common glycoproteins. Here we give an overview about inducers, importance, and relevance of anti-N-Glycan CCD IgE antibodies. Pollen exposure and insect stings induce anti-CCD IgE antibodies interfering with in-vitro tests for allergy diagnosis due to extensive IgE cross-reactivity. Instead of being biologically active these antibodies are irrelevant for allergic reactions due to hymenoptera stings. The general response of the immune system to the ubiquitous exposure to N-glycan containing glycoproteins is still a matter of debate. CCD specific IgG antibodies in sera of bee keepers suggest tolerance induction due to high-dose exposure. Tolerance induction by pollen and food glycoproteins has not been proved. Hymenoptera stings and pollen exposure induce anti-CCD IgE. In regard to anaphylaxis due to Hymenoptera stings these antibodies are not clinically relevant, but they are important for the specificity of in-vitro tests proving insect venom allergy. The introduction of component based diagnostic IgE testing improves the specificity of in-vitro tests if proteins devoid of CCD epitopes are used.

Typical effects of laser dazzling CCD camera

NASA Astrophysics Data System (ADS)

Zhang, Zhen; Zhang, Jianmin; Shao, Bibo; Cheng, Deyan; Ye, Xisheng; Feng, Guobin

2015-05-01

In this article, an overview of laser dazzling effect to buried channel CCD camera is given. The CCDs are sorted into staring and scanning types. The former includes the frame transfer and interline transfer types. The latter includes linear and time delay integration types. All CCDs must perform four primary tasks in generating an image, which are called charge generation, charge collection, charge transfer and charge measurement. In camera, the lenses are needed to input the optical signal to the CCD sensors, in which the techniques for erasing stray light are used. And the electron circuits are needed to process the output signal of CCD, in which many electronic techniques are used. The dazzling effects are the conjunct result of light distribution distortion and charge distribution distortion, which respectively derive from the lens and the sensor. Strictly speaking, in lens, the light distribution is not distorted. In general, the lens are so well designed and fabricated that its stray light can be neglected. But the laser is of much enough intensity to make its stray light obvious. In CCD image sensors, laser can induce a so large electrons generation. Charges transfer inefficiency and charges blooming will cause the distortion of the charge distribution. Commonly, the largest signal outputted from CCD sensor is restricted by capability of the collection well of CCD, and can't go beyond the dynamic range for the subsequent electron circuits maintaining normal work. So the signal is not distorted in the post-processing circuits. But some techniques in the circuit can make some dazzling effects present different phenomenon in final image.

CCD image sensor induced error in PIV applications

NASA Astrophysics Data System (ADS)

Legrand, M.; Nogueira, J.; Vargas, A. A.; Ventas, R.; Rodríguez-Hidalgo, M. C.

2014-06-01

The readout procedure of charge-coupled device (CCD) cameras is known to generate some image degradation in different scientific imaging fields, especially in astrophysics. In the particular field of particle image velocimetry (PIV), widely extended in the scientific community, the readout procedure of the interline CCD sensor induces a bias in the registered position of particle images. This work proposes simple procedures to predict the magnitude of the associated measurement error. Generally, there are differences in the position bias for the different images of a certain particle at each PIV frame. This leads to a substantial bias error in the PIV velocity measurement (˜0.1 pixels). This is the order of magnitude that other typical PIV errors such as peak-locking may reach. Based on modern CCD technology and architecture, this work offers a description of the readout phenomenon and proposes a modeling for the CCD readout bias error magnitude. This bias, in turn, generates a velocity measurement bias error when there is an illumination difference between two successive PIV exposures. The model predictions match the experiments performed with two 12-bit-depth interline CCD cameras (MegaPlus ES 4.0/E incorporating the Kodak KAI-4000M CCD sensor with 4 megapixels). For different cameras, only two constant values are needed to fit the proposed calibration model and predict the error from the readout procedure. Tests by different researchers using different cameras would allow verification of the model, that can be used to optimize acquisition setups. Simple procedures to obtain these two calibration values are also described.

Effects of biomechanical forces on signaling in the cortical collecting duct (CCD)

PubMed Central

Carrisoza-Gaytan, Rolando; Liu, Yu; Flores, Daniel; Else, Cindy; Lee, Heon Goo; Rhodes, George; Sandoval, Ruben M.; Kleyman, Thomas R.; Lee, Francis Young-In; Molitoris, Bruce; Satlin, Lisa M.

2014-01-01

An increase in tubular fluid flow rate (TFF) stimulates Na reabsorption and K secretion in the cortical collecting duct (CCD) and subjects cells therein to biomechanical forces including fluid shear stress (FSS) and circumferential stretch (CS). Intracellular MAPK and extracellular autocrine/paracrine PGE2 signaling regulate cation transport in the CCD and, at least in other systems, are affected by biomechanical forces. We hypothesized that FSS and CS differentially affect MAPK signaling and PGE2 release to modulate cation transport in the CCD. To validate that CS is a physiological force in vivo, we applied the intravital microscopic approach to rodent kidneys in vivo to show that saline or furosemide injection led to a 46.5 ± 2.0 or 170 ± 32% increase, respectively, in distal tubular diameter. Next, murine CCD (mpkCCD) cells were grown on glass or silicone coated with collagen type IV and subjected to 0 or 0.4 dyne/cm2 of FSS or 10% CS, respectively, forces chosen based on prior biomechanical modeling of ex vivo microperfused CCDs. Cells exposed to FSS expressed an approximately twofold greater abundance of phospho(p)-ERK and p-p38 vs. static cells, while CS did not alter p-p38 and p-ERK expression compared with unstretched controls. FSS induced whereas CS reduced PGE2 release by ∼40%. In conclusion, FSS and CS differentially affect ERK and p38 activation and PGE2 release in a cell culture model of the CD. We speculate that TFF differentially regulates biomechanical signaling and, in turn, cation transport in the CCD. PMID:24872319

A Novel Truncating LMNA Mutation in Patients with Cardiac Conduction Disorders and Dilated Cardiomyopathy.

PubMed

Kawakami, Hiroshi; Ogimoto, Akiyoshi; Tokunaga, Naohito; Nishimura, Kazuhisa; Kawakami, Hideo; Higashi, Haruhiko; Iio, Chiharuko; Kono, Tamami; Aono, Jun; Uetani, Teruyoshi; Nagai, Takayuki; Inoue, Katsuji; Suzuki, Jun; Ikeda, Shuntaro; Okura, Takafumi; Ohyagi, Yasumasa; Tabara, Yasuharu; Higaki, Jitsuo

2018-05-30

The cardiac phenotype of laminopathies is characterized by cardiac conduction disorders (CCDs) and dilated cardiomyopathy (DCM). Although laminopathies have been considered monogenic, they exhibit a remarkable degree of clinical variability. This case series aimed to detect the causal mutation and to investigate the causes of clinical variability in a Japanese family with inherited CCD and DCM.Of the five family members investigated, four had either CCD/DCM or CCD alone, while one subject had no cardiovascular disease and acted as a normal control. We performed targeted resequencing of 174 inherited cardiovascular disease-associated genes in this family and pathological mutations were confirmed using Sanger sequencing. The degree of clinical severity and variability were also evaluated using long-term medical records. We discovered a novel heterozygous truncating lamin A/C (LMNA) mutation (c.774delG) in all four subjects with CCD. Because this mutation was predicted to cause a frameshift mutation and premature termination (p.Gln258HisfsTer222) in LMNA, we believe that this LMNA mutation was the causal mutation in this family with CCD and laminopathies. In addition, gender-specific intra-familiar clinical variability was observed in this Japanese family where affected males exhibited an earlier onset of CCD and more severe DCM compared to affected females. Using targeted resequencing, we discovered a novel truncating LMNA mutation associated with CCD and DCM in this family characterized by gender differences in clinical severity in LMNA carriers. Our results suggest that in patients with laminopathy, clinical severity may be the result of multiple factors.

Significantly reducing the processing times of high-speed photometry data sets using a distributed computing model

NASA Astrophysics Data System (ADS)

Doyle, Paul; Mtenzi, Fred; Smith, Niall; Collins, Adrian; O'Shea, Brendan

2012-09-01

The scientific community is in the midst of a data analysis crisis. The increasing capacity of scientific CCD instrumentation and their falling costs is contributing to an explosive generation of raw photometric data. This data must go through a process of cleaning and reduction before it can be used for high precision photometric analysis. Many existing data processing pipelines either assume a relatively small dataset or are batch processed by a High Performance Computing centre. A radical overhaul of these processing pipelines is required to allow reduction and cleaning rates to process terabyte sized datasets at near capture rates using an elastic processing architecture. The ability to access computing resources and to allow them to grow and shrink as demand fluctuates is essential, as is exploiting the parallel nature of the datasets. A distributed data processing pipeline is required. It should incorporate lossless data compression, allow for data segmentation and support processing of data segments in parallel. Academic institutes can collaborate and provide an elastic computing model without the requirement for large centralized high performance computing data centers. This paper demonstrates how a base 10 order of magnitude improvement in overall processing time has been achieved using the "ACN pipeline", a distributed pipeline spanning multiple academic institutes.

Composite targets in HiPIMS plasmas: Correlation of in-vacuum XPS characterization and optical plasma diagnostics

NASA Astrophysics Data System (ADS)

Layes, Vincent; Monje, Sascha; Corbella, Carles; Schulz-von der Gathen, Volker; von Keudell, Achim; de los Arcos, Teresa

2017-05-01

In-vacuum characterization of magnetron targets after High Power Impulse Magnetron Sputtering (HiPIMS) has been performed by X-ray photoelectron spectroscopy (XPS). Al-Cr composite targets (circular, 50 mm diameter) mounted in two different geometries were investigated: an Al target with a small Cr disk embedded at the racetrack position and a Cr target with a small Al disk embedded at the racetrack position. The HiPIMS discharge and the target surface composition were characterized in parallel for low, intermediate, and high power conditions, thus covering both the Ar-dominated and the metal-dominated HiPIMS regimes. The HiPIMS plasma was investigated using optical emission spectroscopy and fast imaging using a CCD camera; the spatially resolved XPS surface characterization was performed after in-vacuum transfer of the magnetron target to the XPS chamber. This parallel evaluation showed that (i) target redeposition of sputtered species was markedly more effective for Cr atoms than for Al atoms; (ii) oxidation at the target racetrack was observed even though the discharge ran in pure Ar gas without O2 admixture, the oxidation depended on the discharge power and target composition; and (iii) a bright emission spot fixed on top of the inserted Cr disk appeared for high power conditions.

Cultural concepts of distress and psychiatric disorders: literature review and research recommendations for global mental health epidemiology

PubMed Central

Kohrt, Brandon A; Rasmussen, Andrew; Kaiser, Bonnie N; Haroz, Emily E; Maharjan, Sujen M; Mutamba, Byamah B; de Jong, Joop TVM; Hinton, Devon E

2014-01-01

Background Burgeoning global mental health endeavors have renewed debates about cultural applicability of psychiatric categories. This study’s goal is to review strengths and limitations of literature comparing psychiatric categories with cultural concepts of distress (CCD) such as cultural syndromes, culture-bound syndromes, and idioms of distress. Methods The Systematic Assessment of Quality in Observational Research (SAQOR) was adapted based on cultural psychiatry principles to develop a Cultural Psychiatry Epidemiology version (SAQOR-CPE), which was used to rate quality of quantitative studies comparing CCD and psychiatric categories. A meta-analysis was performed for each psychiatric category. Results Forty-five studies met inclusion criteria, with 18 782 unique participants. Primary objectives of the studies included comparing CCD and psychiatric disorders (51%), assessing risk factors for CCD (18%) and instrument validation (16%). Only 27% of studies met SAQOR-CPE criteria for medium quality, with the remainder low or very low quality. Only 29% of studies employed representative samples, 53% used validated outcome measures, 44% included function assessments and 44% controlled for confounding. Meta-analyses for anxiety, depression, PTSD and somatization revealed high heterogeneity (I2 > 75%). Only general psychological distress had low heterogeneity (I2 = 8%) with a summary effect odds ratio of 5.39 (95% CI 4.71-6.17). Associations between CCD and psychiatric disorders were influenced by methodological issues, such as validation designs (β = 16.27, 95%CI 12.75-19.79) and use of CCD multi-item checklists (β = 6.10, 95%CI 1.89-10.31). Higher quality studies demonstrated weaker associations of CCD and psychiatric disorders. Conclusions Cultural concepts of distress are not inherently unamenable to epidemiological study. However, poor study quality impedes conceptual advancement and service application. With improved study design and reporting using guidelines such as the SAQOR-CPE, CCD research can enhance detection of mental health problems, reduce cultural biases in diagnostic criteria and increase cultural salience of intervention trial outcomes. PMID:24366490

The design and development of low- and high-voltage ASICs for space-borne CCD cameras

NASA Astrophysics Data System (ADS)

Waltham, N.; Morrissey, Q.; Clapp, M.; Bell, S.; Jones, L.; Torbet, M.

2017-12-01

The CCD remains the pre-eminent visible and UV wavelength image sensor in space science, Earth and planetary remote sensing. However, the design of space-qualified CCD readout electronics is a significant challenge with requirements for low-volume, low-mass, low-power, high-reliability and tolerance to space radiation. Space-qualified components are frequently unavailable and up-screened commercial components seldom meet project or international space agency requirements. In this paper, we describe an alternative approach of designing and space-qualifying a series of low- and high-voltage mixed-signal application-specific integrated circuits (ASICs), the ongoing development of two low-voltage ASICs with successful flight heritage, and two new high-voltage designs. A challenging sub-system of any CCD camera is the video processing and digitisation electronics. We describe recent developments to improve performance and tolerance to radiation-induced single event latchup of a CCD video processing ASIC originally developed for NASA's Solar Terrestrial Relations Observatory and Solar Dynamics Observatory. We also describe a programme to develop two high-voltage ASICs to address the challenges presented with generating a CCD's bias voltages and drive clocks. A 0.35 μm, 50 V tolerant, CMOS process has been used to combine standard low-voltage 3.3 V transistors with high-voltage 50 V diffused MOSFET transistors that enable output buffers to drive CCD bias drains, gates and clock electrodes directly. We describe a CCD bias voltage generator ASIC that provides 24 independent and programmable 0-32 V outputs. Each channel incorporates a 10-bit digital-to-analogue converter, provides current drive of up to 20 mA into loads of 10 μF, and includes current-limiting and short-circuit protection. An on-chip telemetry system with a 12-bit analogue-to-digital converter enables the outputs and multiple off-chip camera voltages to be monitored. The ASIC can drive one or more CCDs and replaces the many discrete components required in current cameras. We also describe a CCD clock driver ASIC that provides six independent and programmable drivers with high-current capacity. The device enables various CCD clock parameters to be programmed independently, for example the clock-low and clock-high voltage levels, and the clock-rise and clock-fall times, allowing configuration for serial clock frequencies in the range 0.1-2 MHz and image clock frequencies in the range 10-100 kHz. Finally, we demonstrate the impact and importance of this technology for the development of compact, high-performance and low-power integrated focal plane electronics.

A Tracking Sun Photometer Without Moving Parts

NASA Technical Reports Server (NTRS)

Strawa, Anthony W.

2012-01-01

This innovation is small, lightweight, and consumes very little electricity as it measures the solar energy attenuated by gases and aerosol particles in the atmosphere. A Sun photometer is commonly used on the Earth's surface, as well as on aircraft, to determine the solar energy attenuated by aerosol particles in the atmosphere and their distribution of sizes. This information is used to determine the spatial and temporal distribution of gases and aerosols in the atmosphere, as well as their distribution sizes. The design for this Sun photometer uses a combination of unique optics and a charge coupled device (CCD) array to eliminate moving parts and make the instrument more reliable. It could be selfcalibrating throughout the year. Data products would be down-welling flux, the direct-diffuse flux ratio, column abundance of gas phase constituents, aerosol optical depth at multiple-wavelengths, phase functions, cloud statistics, and an estimate of the representative size of atmospheric particles. These measurements can be used to obtain an estimate of aerosol size distribution, refractive index, and particle shape. Incident light is received at a light-reflecting (inner) surface, which is a truncated paraboloid. Light arriving from a hemispheric field of view (solid angle 2 steradians) enters the reflecting optic at an entrance aperture at, or adjacent to, the focus of the paraboloid, and is captured by the optic. Most of this light is reflected from an inner surface. The light proceeds substantially parallel to the paraboloid axis, and is detected by an array detector located near an exit aperture. Each of the entrance and exit apertures is formed by the intersection of the paraboloid with a plane substantially perpendicular to the paraboloid axis. Incident (non-reflected) light from a source of limited extent (the Sun) illuminates a limited area on the detector array. Both direct and diffuse illumination may be reflected, or not reflected, before being received on the detector array. As the Sun traverses a path in the sky over some time interval, the track of the Sun can be traced on the detector array. A suitably modified Sun photometer might be used to study the dynamics of an environment on another planet or satellite with an atmosphere.

Characterization of fracture aperture for groundwater flow and transport

NASA Astrophysics Data System (ADS)

Sawada, A.; Sato, H.; Tetsu, K.; Sakamoto, K.

2007-12-01

This paper presents experiments and numerical analyses of flow and transport carried out on natural fractures and transparent replica of fractures. The purpose of this study was to improve the understanding of the role of heterogeneous aperture patterns on channelization of groundwater flow and dispersion in solute transport. The research proceeded as follows: First, a precision plane grinder was applied perpendicular to the fracture plane to characterize the aperture distribution on a natural fracture with 1 mm of increment size. Although both time and labor were intensive, this approach provided a detailed, three dimensional picture of the pattern of fracture aperture. This information was analyzed to provide quantitative measures for the fracture aperture distribution, including JRC (Joint Roughness Coefficient) and fracture contact area ratio. These parameters were used to develop numerical models with corresponding synthetic aperture patterns. The transparent fracture replica and numerical models were then used to study how transport is affected by the aperture spatial pattern. In the transparent replica, transmitted light intensity measured by a CCD camera was used to image channeling and dispersion due to the fracture aperture spatial pattern. The CCD image data was analyzed to obtain the quantitative fracture aperture and tracer concentration data according to Lambert-Beer's law. The experimental results were analyzed using the numerical models. Comparison of the numerical models to the transparent replica provided information about the nature of channeling and dispersion due to aperture spatial patterns. These results support to develop a methodology for defining representative fracture aperture of a simplified parallel fracture model for flow and transport in heterogeneous fractures for contaminant transport analysis.

Atomic resolution characterization of a SrTiO{sub 3} grain boundary in the STEM

DOE Office of Scientific and Technical Information (OSTI.GOV)

McGibbon, M.M.; Browning, N.D.; Chisholm, M.F.

This paper uses the complementary techniques of high resolution Z-contrast imaging and PEELS (parallel detection electron energy loss spectroscopy) to investigate the atomic structure and chemistry of a 25 degree symmetric tilt boundary in a bicrystal of the electroceramic SrTiO{sub 3}. The gain boundary is composed of two different boundary structural units which occur in about equal numbers: one which contains Ti-O columns and the other without.

Rotating columns: Relating structure-from-motion, accretion/deletion, and figure/ground

PubMed Central

Froyen, Vicky; Feldman, Jacob; Singh, Manish

2013-01-01

We present a novel phenomenon involving an interaction between accretion deletion, figure-ground interpretation, and structure-from-motion. Our displays contain alternating light and dark vertical regions in which random-dot textures moved horizontally at constant speed but in opposite directions in alternating regions. This motion is consistent with all the light regions in front, with the dark regions completing amodally into a single large surface moving in the background, or vice versa. Surprisingly, the regions that are perceived as figural are also perceived as 3-D volumes rotating in depth (like rotating columns)—despite the fact that dot motion is not consistent with 3-D rotation. In a series of experiments, we found we could manipulate which set of regions is perceived as rotating volumes simply by varying known geometric cues to figure ground, including convexity, parallelism, symmetry, and relative area. Subjects indicated which colored regions they perceived as rotating. For our displays we found convexity to be a stronger cue than either symmetry or parallelism. We furthermore found a smooth monotonic decay of the proportion by which subjects perceive symmetric regions as figural, as a function of their relative area. Our results reveal an intriguing new interaction between accretion-deletion, figure-ground, and 3-D motion that is not captured by existing models. They also provide an effective tool for measuring figure-ground perception. PMID:23946432

Rotating columns: relating structure-from-motion, accretion/deletion, and figure/ground.

PubMed

Froyen, Vicky; Feldman, Jacob; Singh, Manish

2013-08-14

We present a novel phenomenon involving an interaction between accretion deletion, figure-ground interpretation, and structure-from-motion. Our displays contain alternating light and dark vertical regions in which random-dot textures moved horizontally at constant speed but in opposite directions in alternating regions. This motion is consistent with all the light regions in front, with the dark regions completing amodally into a single large surface moving in the background, or vice versa. Surprisingly, the regions that are perceived as figural are also perceived as 3-D volumes rotating in depth (like rotating columns)-despite the fact that dot motion is not consistent with 3-D rotation. In a series of experiments, we found we could manipulate which set of regions is perceived as rotating volumes simply by varying known geometric cues to figure ground, including convexity, parallelism, symmetry, and relative area. Subjects indicated which colored regions they perceived as rotating. For our displays we found convexity to be a stronger cue than either symmetry or parallelism. We furthermore found a smooth monotonic decay of the proportion by which subjects perceive symmetric regions as figural, as a function of their relative area. Our results reveal an intriguing new interaction between accretion-deletion, figure-ground, and 3-D motion that is not captured by existing models. They also provide an effective tool for measuring figure-ground perception.

A novel approach for epipolar resampling of cross-track linear pushbroom imagery using orbital parameters model

NASA Astrophysics Data System (ADS)

Jannati, Mojtaba; Valadan Zoej, Mohammad Javad; Mokhtarzade, Mehdi

2018-03-01

This paper presents a novel approach to epipolar resampling of cross-track linear pushbroom imagery using orbital parameters model (OPM). The backbone of the proposed method relies on modification of attitude parameters of linear array stereo imagery in such a way to parallelize the approximate conjugate epipolar lines (ACELs) with the instantaneous base line (IBL) of the conjugate image points (CIPs). Afterward, a complementary rotation is applied in order to parallelize all the ACELs throughout the stereo imagery. The new estimated attitude parameters are evaluated based on the direction of the IBL and the ACELs. Due to the spatial and temporal variability of the IBL (respectively changes in column and row numbers of the CIPs) and nonparallel nature of the epipolar lines in the stereo linear images, some polynomials in the both column and row numbers of the CIPs are used to model new attitude parameters. As the instantaneous position of sensors remains fix, the digital elevation model (DEM) of the area of interest is not required in the resampling process. According to the experimental results obtained from two pairs of SPOT and RapidEye stereo imagery with a high elevation relief, the average absolute values of remained vertical parallaxes of CIPs in the normalized images were obtained 0.19 and 0.28 pixels respectively, which confirm the high accuracy and applicability of the proposed method.

A Fast Multiple Sampling Method for Low-Noise CMOS Image Sensors With Column-Parallel 12-bit SAR ADCs

PubMed Central

Kim, Min-Kyu; Hong, Seong-Kwan; Kwon, Oh-Kyong

2015-01-01

This paper presents a fast multiple sampling method for low-noise CMOS image sensor (CIS) applications with column-parallel successive approximation register analog-to-digital converters (SAR ADCs). The 12-bit SAR ADC using the proposed multiple sampling method decreases the A/D conversion time by repeatedly converting a pixel output to 4-bit after the first 12-bit A/D conversion, reducing noise of the CIS by one over the square root of the number of samplings. The area of the 12-bit SAR ADC is reduced by using a 10-bit capacitor digital-to-analog converter (DAC) with four scaled reference voltages. In addition, a simple up/down counter-based digital processing logic is proposed to perform complex calculations for multiple sampling and digital correlated double sampling. To verify the proposed multiple sampling method, a 256 × 128 pixel array CIS with 12-bit SAR ADCs was fabricated using 0.18 μm CMOS process. The measurement results shows that the proposed multiple sampling method reduces each A/D conversion time from 1.2 μs to 0.45 μs and random noise from 848.3 μV to 270.4 μV, achieving a dynamic range of 68.1 dB and an SNR of 39.2 dB. PMID:26712765

Development of a CCD array as an imaging detector for advanced X-ray astrophysics facilities

NASA Technical Reports Server (NTRS)

Schwartz, D. A.

1981-01-01

The development of a charge coupled device (CCD) X-ray imager for a large aperture, high angular resolution X-ray telescope is discussed. Existing CCDs were surveyed and three candidate concepts were identified. An electronic camera control and computer interface, including software to drive a Fairchild 211 CCD, is described. In addition a vacuum mounting and cooling system is discussed. Performance data for the various components are given.

CCD Astrometry with Robotic Telescopes

NASA Astrophysics Data System (ADS)

AlZaben, Faisal; Li, Dewei; Li, Yongyao; Dennis, Aren Fene, Michael; Boyce, Grady; Boyce, Pat

2016-01-01

CCD images were acquired of three binary star systems: WDS06145+1148, WDS06206+1803, and WDS06224+2640. The astrometric solution, position angle, and separation of each system were calculated with MaximDL v6 and Mira Pro x64 software suites. The results were consistent with historical measurements in the Washington Double Star Catalog. Our analysis found some differences in measurements between single-shot color CCD cameras and traditional monochrome CCDs using a filter wheel.

A FORTRAN version implementation of block adjustment of CCD frames and its preliminary application

NASA Astrophysics Data System (ADS)

Yu, Y.; Tang, Z.-H.; Li, J.-L.; Zhao, M.

2005-09-01

A FORTRAN version implementation of the block adjustment (BA) of overlapping CCD frames is developed and its flowchart is shown. The program is preliminarily applied to obtain the optical positions of four extragalactic radio sources. The results show that because of the increase in the number and sky coverage of reference stars the precision of optical positions with BA is improved compared with the single CCD frame adjustment.

Applications of charge-coupled device transversal filters to communication

NASA Technical Reports Server (NTRS)

Buss, D. D.; Bailey, W. H.; Brodersen, R. W.; Hewes, C. R.; Tasch, A. F., Jr.

1975-01-01

The paper discusses the computational power of state-of-the-art charged-coupled device (CCD) transversal filters in communications applications. Some of the performance limitations of CCD transversal filters are discussed, with attention given to time delay and bandwidth, imperfect charge transfer efficiency, weighting coefficient error, noise, and linearity. The application of CCD transversal filters to matched filtering, spectral filtering, and Fourier analysis is examined. Techniques for making programmable transversal filters are briefly outlined.

CCD imaging system for the EUV solar telescope

NASA Astrophysics Data System (ADS)

Gong, Yan; Song, Qian; Ye, Bing-Xun

2006-01-01

In order to develop the detector adapted to the space solar telescope, we have built a CCD camera system capable of working in the extra ultraviolet (EUV) band, which is composed of one phosphor screen, one intensified system using a photocathode/micro-channel plate(MCP)/ phosphor, one optical taper and one chip of front-illuminated (FI) CCD without screen windows. All of them were stuck one by one with optical glue. The working principle of the camera system is presented; moreover we have employed the mesh experiment to calibrate and test the CCD camera system in 15~24nm, the position resolution of about 19 μm is obtained at the wavelength of 17.1nm and 19.5nm.

CCD centroiding analysis for Nano-JASMINE observation data

NASA Astrophysics Data System (ADS)

Niwa, Yoshito; Yano, Taihei; Araki, Hiroshi; Gouda, Naoteru; Kobayashi, Yukiyasu; Yamada, Yoshiyuki; Tazawa, Seiichi; Hanada, Hideo

2010-07-01

Nano-JASMINE is a very small satellite mission for global space astrometry with milli-arcsecond accuracy, which will be launched in 2011. In this mission, centroids of stars in CCD image frames are estimated with sub-pixel accuracy. In order to realize such a high precision centroiding an algorithm utilizing a least square method is employed. One of the advantages is that centroids can be calculated without explicit assumption of the point spread functions of stars. CCD centroiding experiment has been performed to investigate whether this data analysis is available, and centroids of artificial star images on a CCD are determined with a precision of less than 0.001 pixel. This result indicates parallaxes of stars within 300 pc from Sun can be observed in Nano-JASMINE.

Printed circuit board for a CCD camera head

DOEpatents

Conder, Alan D.

2002-01-01

A charge-coupled device (CCD) camera head which can replace film for digital imaging of visible light, ultraviolet radiation, and soft to penetrating x-rays, such as within a target chamber where laser produced plasmas are studied. The camera head is small, capable of operating both in and out of a vacuum environment, and is versatile. The CCD camera head uses PC boards with an internal heat sink connected to the chassis for heat dissipation, which allows for close (0.04" for example) stacking of the PC boards. Integration of this CCD camera head into existing instrumentation provides a substantial enhancement of diagnostic capabilities for studying high energy density plasmas, for a variety of military industrial, and medical imaging applications.

CCD BVI c observations of Cepheids

NASA Astrophysics Data System (ADS)

Berdnikov, L. N.; Kniazev, A. Yu.; Sefako, R.; Kravtsov, V. V.; Zhujko, S. V.

2014-02-01

In 2008-2013, we obtained 11333 CCD BVI c frames for 57 Cepheids from the General Catalogue of Variable Stars. We performed our observations with the 76-cm telescope of the South African Astronomical Observatory (SAAO, South Africa) and the 40-cm telescope of the Cerro Armazones Astronomical Observatory of the Universidad Católica del Norte (OCA, Chile) using the SBIG ST-10XME CCD camera. The tables of observations, the plots of light curves, and the current light elements are presented. Comparison of our light curves with those constructed from photoelectric observations shows that the differences between their mean magnitudes exceed 0ṃ05 in 20% of the cases. This suggests the necessity of performing CCD observations for all Cepheids.

Optical detection of two-color-fluorophore barcode for nanopore DNA sensing

NASA Astrophysics Data System (ADS)

Zhang, M.; Sychugov, I.; Schmidt, T.; Linnros, J.

2015-06-01

A simple schematic on parallel optical detection of two-fluorophore barcode for single-molecule nanopore sensing is presented. The chosen two fluorophores, ATTO-532 and DY-521-XL, emitting in well-separated spectrum range can be excited at the same wavelength. A beam splitter was employed to separate signals from the two fluorophores and guide them to the same CCD camera. Based on a conventional microscope, sources of background in the nanopore sensing system, including membranes, compounds in buffer solution, and a detection cell was characterized. By photoluminescence excitation measurements, it turned out that silicon membrane has a negligible photoluminescence under the examined excitation from 440 nm to 560 nm, in comparison with a silicon nitrite membrane. Further, background signals from the detection cell were suppressed. Brownian motion of 450 bps DNA labelled with single ATTO-532 or DY-521-XL was successfully recorded by our optical system.

Reflectometer for pseudo-Brewster angle spectrometry (BAIRS)

NASA Astrophysics Data System (ADS)

Potter, Roy F.

2000-10-01

A simple, robust reflectometer, pre-set for several angles of incidence (AOI), has been designed and used for determining the optical parameters of opaque samples having a specular surface. A single, linear polarizing element permits the measurement of perpendicular(s) and parallel (p) reflectence at each AOI. The BAIRS algorithm determines the empirical optical parameters for the subject surface at the pseudo-Brewster AOI, based on the measurement of p/s at two AOI's and, in turn the optical constants n and k (or (epsilon) 1 and (epsilon) 2). Radiation sources in current use, are a stabilized tungsten-halide lamp or a deuterium lamp for the visible and near UV spectral regions. Silica fiber optics and lenses deliver input and output radiation from the source and to a CCD array scanned diffraction spectrometer. Results for a sample of GaAs will be presented along with a discussion of dispersion features in the optical constant spectra.

Optical spatial differentiator based on subwavelength high-contrast gratings

NASA Astrophysics Data System (ADS)

Dong, Zhewei; Si, Jiangnan; Yu, Xuanyi; Deng, Xiaoxu

2018-04-01

An optical spatial differentiator based on subwavelength high-contrast gratings (HCGs) is proposed experimentally. The spatial differentiation property of the subwavelength HCG is analyzed by calculating its spatial spectral transfer function based on the periodic waveguide theory. By employing the FDTD solutions, the performance of the subwavelength HCG spatial differentiator was investigated numerically. The subwavelength HCG differentiator with the thickness at the nanoscale was fabricated on the quartz substrate by electron beam lithography and Bosch deep silicon etching. Observed under an optical microscope with a CCD camera, the spatial differentiation of the incident field profile was obtained by the subwavelength HCG differentiator in transmission without Fourier lens. By projecting the images of slits, letter "X," and a cross on the subwavelength HCG differentiator, edge detections of images were obtained in transmission. With the nanoscale HCG structure and simple optical implementation, the proposed optical spatial differentiator provides the prospects for applications in optical computing systems and parallel data processing.

Mach Probe Measurements in a Large-Scale Helicon Plasma

NASA Astrophysics Data System (ADS)

Hatch, M. W.; Kelly, R. F.; Fisher, D. M.; Gilmore, M.; Dwyer, R. H.

2017-10-01

A new six-tipped Mach probe, that utilizes a fused-quartz insulator, has been developed and initially tested in the HelCat dual-source plasma device at the University of New Mexico. The new design allows for relatively long duration measurements of parallel and perpendicular flows that suffer less from thermal changes in conductivity and surface build-up seen in previous alumina-insulated designs. Mach probe measurement will be presented in comparison with ongoing laser induced fluorescence (LIF) measurements, previous Mach probe measurements, ExB flow estimates derived from Langmuir probes, and fast-frame CCD camera images, in an effort to better understand previous anomalous ion flow in HelCat. Additionally, Mach probe-LIF comparisons will provide an experimentally obtained Mach probe calibration constant, K, to validate sheath-derived estimates for the weakly magnetized case. Supported by U.S. National Science Foundation Award 1500423.

Molecular Imaging in the College of Optical Sciences – An Overview of Two Decades of Instrumentation Development

PubMed Central

Furenlid, Lars R.; Barrett, Harrison H.; Barber, H. Bradford; Clarkson, Eric W.; Kupinski, Matthew A.; Liu, Zhonglin; Stevenson, Gail D.; Woolfenden, James M.

2015-01-01

During the past two decades, researchers at the University of Arizona’s Center for Gamma-Ray Imaging (CGRI) have explored a variety of approaches to gamma-ray detection, including scintillation cameras, solid-state detectors, and hybrids such as the intensified Quantum Imaging Device (iQID) configuration where a scintillator is followed by optical gain and a fast CCD or CMOS camera. We have combined these detectors with a variety of collimation schemes, including single and multiple pinholes, parallel-hole collimators, synthetic apertures, and anamorphic crossed slits, to build a large number of preclinical molecular-imaging systems that perform Single-Photon Emission Computed Tomography (SPECT), Positron Emission Tomography (PET), and X-Ray Computed Tomography (CT). In this paper, we discuss the themes and methods we have developed over the years to record and fully use the information content carried by every detected gamma-ray photon. PMID:26236069

Handling of huge multispectral image data volumes from a spectral hole burning device (SHBD)

NASA Astrophysics Data System (ADS)

Graff, Werner; Rosselet, Armel C.; Wild, Urs P.; Gschwind, Rudolf; Keller, Christoph U.

1995-06-01

We use chlorin-doped polymer films at low temperatures as the primary imaging detector. Based on the principles of persistent spectral hole burning, this system is capable of storing spatial and spectral information simultaneously in one exposure with extremely high resolution. The sun as an extended light source has been imaged onto the film. The information recorded amounts to tens of GBytes. This data volume is read out by scanning the frequency of a tunable dye laser and reading the images with a digital CCD camera. For acquisition, archival, processing, and visualization, we use MUSIC (MUlti processor System with Intelligent Communication), a single instruction multiple data parallel processor system equipped with the necessary I/O facilities. The huge amount of data requires the developemnt of sophisticated algorithms to efficiently calibrate the data and to extract useful and new information for solar physics.

Fabrication of demultiplexer for T bps optical signals by using spincoated squarylium dye J-aggregates exhibiting femtosecond optical response

NASA Astrophysics Data System (ADS)

Iwasa, Izumi; Furuki, Makoto; Tian, Minquan; Sato, Yasuhiro; Pu, Lyong S.; Tatsuura, Setoshi; Wada, Osamu

2001-06-01

We fabricated spincoated films of squarylium dye (SQ) J- aggregates exhibiting femtosecond optical response at room temperature. Optical dynamics measurements revealed that the saturable absorption of the SQ J-aggregates film exhibited a decay time of less than 100 fs at a pump energy of 80 fJ/micrometer2. With this ultrafast SQ optical film, four- output demultiplex operation for T bps pulses was demonstrated. A series of 4 optical pulses with 100 fs duration and 1 ps interval (corresponding to 1 T bps signals) were irradiated onto the SQ film synchronized with a 100 fs gate pulse at a finite angle. Four demultiplexed signals were clearly observed at different areas on the CCD camera. Multi- output serial-to-parallel demultiplexer for T bps optical signals can be formed using the SQ J-aggregates film.

New Spectral Model for Constraining Torus Covering Factors from Broadband X-Ray Spectra of Active Galactic Nuclei

NASA Astrophysics Data System (ADS)

Baloković, M.; Brightman, M.; Harrison, F. A.; Comastri, A.; Ricci, C.; Buchner, J.; Gandhi, P.; Farrah, D.; Stern, D.

2018-02-01

The basic unified model of active galactic nuclei (AGNs) invokes an anisotropic obscuring structure, usually referred to as a torus, to explain AGN obscuration as an angle-dependent effect. We present a new grid of X-ray spectral templates based on radiative transfer calculations in neutral gas in an approximately toroidal geometry, appropriate for CCD-resolution X-ray spectra (FWHM ≥ 130 eV). Fitting the templates to broadband X-ray spectra of AGNs provides constraints on two important geometrical parameters of the gas distribution around the supermassive black hole: the average column density and the covering factor. Compared to the currently available spectral templates, our model is more flexible, and capable of providing constraints on the main torus parameters in a wider range of AGNs. We demonstrate the application of this model using hard X-ray spectra from NuSTAR (3–79 keV) for four AGNs covering a variety of classifications: 3C 390.3, NGC 2110, IC 5063, and NGC 7582. This small set of examples was chosen to illustrate the range of possible torus configurations, from disk-like to sphere-like geometries with column densities below, as well as above, the Compton-thick threshold. This diversity of torus properties challenges the simple assumption of a standard geometrically and optically thick toroidal structure commonly invoked in the basic form of the unified model of AGNs. Finding broad consistency between our constraints and those from infrared modeling, we discuss how the approach from the X-ray band complements similar measurements of AGN structures at other wavelengths.

Fixed Pattern Noise pixel-wise linear correction for crime scene imaging CMOS sensor

NASA Astrophysics Data System (ADS)

Yang, Jie; Messinger, David W.; Dube, Roger R.; Ientilucci, Emmett J.

2017-05-01

Filtered multispectral imaging technique might be a potential method for crime scene documentation and evidence detection due to its abundant spectral information as well as non-contact and non-destructive nature. Low-cost and portable multispectral crime scene imaging device would be highly useful and efficient. The second generation crime scene imaging system uses CMOS imaging sensor to capture spatial scene and bandpass Interference Filters (IFs) to capture spectral information. Unfortunately CMOS sensors suffer from severe spatial non-uniformity compared to CCD sensors and the major cause is Fixed Pattern Noise (FPN). IFs suffer from "blue shift" effect and introduce spatial-spectral correlated errors. Therefore, Fixed Pattern Noise (FPN) correction is critical to enhance crime scene image quality and is also helpful for spatial-spectral noise de-correlation. In this paper, a pixel-wise linear radiance to Digital Count (DC) conversion model is constructed for crime scene imaging CMOS sensor. Pixel-wise conversion gain Gi,j and Dark Signal Non-Uniformity (DSNU) Zi,j are calculated. Also, conversion gain is divided into four components: FPN row component, FPN column component, defects component and effective photo response signal component. Conversion gain is then corrected to average FPN column and row components and defects component so that the sensor conversion gain is uniform. Based on corrected conversion gain and estimated image incident radiance from the reverse of pixel-wise linear radiance to DC model, corrected image spatial uniformity can be enhanced to 7 times as raw image, and the bigger the image DC value within its dynamic range, the better the enhancement.

Curved CCD detector devices and arrays for multispectral astrophysical applications and terrestrial stereo panoramic cameras

NASA Astrophysics Data System (ADS)

Swain, Pradyumna; Mark, David

2004-09-01

The emergence of curved CCD detectors as individual devices or as contoured mosaics assembled to match the curved focal planes of astronomical telescopes and terrestrial stereo panoramic cameras represents a major optical design advancement that greatly enhances the scientific potential of such instruments. In altering the primary detection surface within the telescope"s optical instrumentation system from flat to curved, and conforming the applied CCD"s shape precisely to the contour of the telescope"s curved focal plane, a major increase in the amount of transmittable light at various wavelengths through the system is achieved. This in turn enables multi-spectral ultra-sensitive imaging with much greater spatial resolution necessary for large and very large telescope applications, including those involving infrared image acquisition and spectroscopy, conducted over very wide fields of view. For earth-based and space-borne optical telescopes, the advent of curved CCD"s as the principle detectors provides a simplification of the telescope"s adjoining optics, reducing the number of optical elements and the occurrence of optical aberrations associated with large corrective optics used to conform to flat detectors. New astronomical experiments may be devised in the presence of curved CCD applications, in conjunction with large format cameras and curved mosaics, including three dimensional imaging spectroscopy conducted over multiple wavelengths simultaneously, wide field real-time stereoscopic tracking of remote objects within the solar system at high resolution, and deep field survey mapping of distant objects such as galaxies with much greater multi-band spatial precision over larger sky regions. Terrestrial stereo panoramic cameras equipped with arrays of curved CCD"s joined with associative wide field optics will require less optical glass and no mechanically moving parts to maintain continuous proper stereo convergence over wider perspective viewing fields than their flat CCD counterparts, lightening the cameras and enabling faster scanning and 3D integration of objects moving within a planetary terrain environment. Preliminary experiments conducted at the Sarnoff Corporation indicate the feasibility of curved CCD imagers with acceptable electro-optic integrity. Currently, we are in the process of evaluating the electro-optic performance of a curved wafer scale CCD imager. Detailed ray trace modeling and experimental electro-optical data performance obtained from the curved imager will be presented at the conference.

Targeted reversion of induced pluripotent stem cells from patients with human cleidocranial dysplasia improves bone regeneration in a rat calvarial bone defect model.

PubMed

Saito, Akiko; Ooki, Akio; Nakamura, Takashi; Onodera, Shoko; Hayashi, Kamichika; Hasegawa, Daigo; Okudaira, Takahito; Watanabe, Katsuhito; Kato, Hiroshi; Onda, Takeshi; Watanabe, Akira; Kosaki, Kenjiro; Nishimura, Ken; Ohtaka, Manami; Nakanishi, Mahito; Sakamoto, Teruo; Yamaguchi, Akira; Sueishi, Kenji; Azuma, Toshifumi

2018-01-22

Runt-related transcription factor 2 (RUNX2) haploinsufficiency causes cleidocranial dysplasia (CCD) which is characterized by supernumerary teeth, short stature, clavicular dysplasia, and osteoporosis. At present, as a therapeutic strategy for osteoporosis, mesenchymal stem cell (MSC) transplantation therapy is performed in addition to drug therapy. However, MSC-based therapy for osteoporosis in CCD patients is difficult due to a reduction in the ability of MSCs to differentiate into osteoblasts resulting from impaired RUNX2 function. Here, we investigated whether induced pluripotent stem cells (iPSCs) properly differentiate into osteoblasts after repairing the RUNX2 mutation in iPSCs derived from CCD patients to establish normal iPSCs, and whether engraftment of osteoblasts derived from properly reverted iPSCs results in better regeneration in immunodeficient rat calvarial bone defect models. Two cases of CCD patient-derived induced pluripotent stem cells (CCD-iPSCs) were generated using retroviral vectors (OCT3/4, SOX2, KLF4, and c-MYC) or a Sendai virus SeVdp vector (KOSM302L). Reverted iPSCs were established using programmable nucleases, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-derived RNA-guided endonucleases, to correct mutations in CCD-iPSCs. The mRNA expressions of osteoblast-specific markers were analyzed using quantitative reverse-transcriptase polymerase chain reaction. iPSCs-derived osteoblasts were transplanted into rat calvarial bone defects, and bone regeneration was evaluated using microcomputed tomography analysis and histological analysis. Mutation analysis showed that both contained nonsense mutations: one at the very beginning of exon 1 and the other at the initial position of the nuclear matrix-targeting signal. The osteoblasts derived from CCD-iPSCs (CCD-OBs) expressed low levels of several osteoblast differentiation markers, and transplantation of these osteoblasts into calvarial bone defects created in rats with severe combined immunodeficiency showed poor regeneration. However, reverted iPSCs improved the abnormal osteoblast differentiation which resulted in much better engraftment into the rat calvarial bone defect. Taken together, these results demonstrate that patient-specific iPSC technology can not only provide a useful disease model to elucidate the role of RUNX2 in osteoblastic differentiation but also raises the tantalizing prospect that reverted iPSCs might provide a practical medical treatment for CCD.

Radiating columnar joints in Gyeongju, Korea as a educational site

NASA Astrophysics Data System (ADS)

Woo, H.; Kim, J. H.; Jang, Y. D.

2015-12-01

Gyeongju is located in the central eastern part of South Korea. There are various directional columnar joint sets in Tertiary trachytic basalt formation along the shore. In particular, rare radiating columnar joints occur in this area. Columnar joints are parallel, prismatic columns that are formed as a result of contraction during the rapid cooling of lava flow, forming a three dimensional fracture network. In general, the radius and direction of the rock column represent the cooling rate and surface respectively. Radiating direction of columns here indicates that dome- or lobe-shaped lava was cooled from its surface to the core during the viscous lava flow. The fact that the trachytic textures of plagioclase laths are indistinct suggests that the radiating columnar joints are equivalent to the frontal end of the lava lobes. This area is currently has a shore trail course, which is being developed into a picturesque educational park. There are corresponding information boards on the trail near each type of columnar joints to explain not only the forming process and geological mechanisms but the importance of nature conservation to visitors, especially students. A variety of educational materials and educational programs linked to regular school curriculum are also being developed.

Columnar to Nematic Mesophase Transition: Binary Mixtures of Copper Soaps with Hydrocarbons

NASA Astrophysics Data System (ADS)

Seghrouchni, R.; Skoulios, A.

1995-09-01

Copper (II) soaps are known to produce columnar mesophases at high temperatures. The polar groups of the soap molecules are stacked over one another within columns surrounded by the paraffin chains in a disordered conformation and laterally arranged according to a two-dimensional hexagonal lattice. Upon addition of a hydrocarbon, the mesophases swell homogeneously. The hydrocarbon molecules locate themselves among the disordered chains of the soap molecules, the columnar cores remain perfectly unchanged, keeping a constant intra-columnar stacking period, and the hexagonal lattice expands in proportion to the amount of hydrocarbon added to the system. Beyond a certain degree of swelling, the columnar mesophases suddenly turn into a nematic mesophase through a first-order phase transition. The structural elements that align parallel to the nematic director are the very same molecular columns that are involved in the columnar mesophases. The columnar to nematic mesophase transition was studied systematically as a function of the molecular size of the soaps and hydrocarbons used as diluents and discussed on a molecular level, emphasizing such aspects as the persistence length of the paraffin chains and the location of the solvent molecules among the columns.

Column Number Density Expressions Through M = 0 and M = 1 Point Source Plumes Along Any Straight Path

NASA Technical Reports Server (NTRS)

Woronowicz, Michael

2016-01-01

Analytical expressions for column number density (CND) are developed for optical line of sight paths through a variety of steady free molecule point source models including directionally-constrained effusion (Mach number M = 0) and flow from a sonic orifice (M = 1). Sonic orifice solutions are approximate, developed using a fair simulacrum fitted to the free molecule solution. Expressions are also developed for a spherically-symmetric thermal expansion (M = 0). CND solutions are found for the most general paths relative to these sources and briefly explored. It is determined that the maximum CND from a distant location through directed effusion and sonic orifice cases occurs along the path parallel to the source plane that intersects the plume axis. For the effusive case this value is exactly twice the CND found along the ray originating from that point of intersection and extending to infinity along the plume's axis. For sonic plumes this ratio is reduced to about 4/3. For high Mach number cases the maximum CND will be found along the axial centerline path. Keywords: column number density, plume flows, outgassing, free molecule flow.

Bioinformatic and expression analyses on carotenoid dioxygenase genes in fruit development and abiotic stress responses in Fragaria vesca.

PubMed

Wang, Yong; Ding, Guanqun; Gu, Tingting; Ding, Jing; Li, Yi

2017-08-01

Carotenoid dioxygenases, including 9-cis-epoxycarotenoid dioxygenases (NCEDs) and carotenoid cleavage dioxygenases (CCDs), can selectively cleave carotenoids into various apocarotenoid products that play important roles in fleshy fruit development and abiotic stress response. In this study, we identified 12 carotenoid dioxygenase genes in diploid strawberry Fragaria vesca, and explored their evolution with orthologous genes from nine other species. Phylogenetic analyses suggested that the NCED and CCDL groups moderately expanded during their evolution, whereas gene numbers of the CCD1, CCD4, CCD7, and CCD8 groups maintained conserved. We characterized the expression profiles of FveNCED and FveCCD genes during flower and fruit development, and in response to several abiotic stresses. FveNCED1 expression positively responded to osmotic, cold, and heat stresses, whereas FveNCED2 was only induced under cold stress. In contrast, FveNCED2 was the unique gene highly and continuously increasing in receptacle during fruit ripening, which co-occurred with the increase in endogenous abscisic acid (ABA) content previously reported in octoploid strawberry. The differential expression patterns suggested that FveNCED1 and FveNCED2 were key genes for ABA biosynthesis in abiotic stress responses and fruit ripening, respectively. FveCCD1 exhibited the highest expression in most stages of flower and fruit development, while the other FveCCDs were expressed in a subset of stages and tissues. Our study suggests distinct functions of FveNCED and FveCCD genes in fruit development and stress responses and lays a foundation for future study to understand the roles of these genes and their metabolites, including ABA and other apocarotenoid products, in the growth and development of strawberry.

SU-E-T-161: SOBP Beam Analysis Using Light Output of Scintillation Plate Acquired by CCD Camera.

PubMed

Cho, S; Lee, S; Shin, J; Min, B; Chung, K; Shin, D; Lim, Y; Park, S

2012-06-01

To analyze Bragg-peak beams in SOBP (spread-out Bragg-peak) beam using CCD (charge-coupled device) camera - scintillation screen system. We separated each Bragg-peak beam using light output of high sensitivity scintillation material acquired by CCD camera and compared with Bragg-peak beams calculated by Monte Carlo simulation. In this study, CCD camera - scintillation screen system was constructed with a high sensitivity scintillation plate (Gd2O2S:Tb) and a right-angled prismatic PMMA phantom, and a Marlin F-201B, EEE-1394 CCD camera. SOBP beam irradiated by the double scattering mode of a PROTEUS 235 proton therapy machine in NCC is 8 cm width, 13 g/cm 2 range. The gain, dose rate and current of this beam is 50, 2 Gy/min and 70 nA, respectively. Also, we simulated the light output of scintillation plate for SOBP beam using Geant4 toolkit. We evaluated the light output of high sensitivity scintillation plate according to intergration time (0.1 - 1.0 sec). The images of CCD camera during the shortest intergration time (0.1 sec) were acquired automatically and randomly, respectively. Bragg-peak beams in SOBP beam were analyzed by the acquired images. Then, the SOBP beam used in this study was calculated by Geant4 toolkit and Bragg-peak beams in SOBP beam were obtained by ROOT program. The SOBP beam consists of 13 Bragg-peak beams. The results of experiment were compared with that of simulation. We analyzed Bragg-peak beams in SOBP beam using light output of scintillation plate acquired by CCD camera and compared with that of Geant4 simulation. We are going to study SOBP beam analysis using more effective the image acquisition technique. © 2012 American Association of Physicists in Medicine.

Molecular cloning and characterization of cDNAs encoding carotenoid cleavage dioxygenase in bitter melon (Momordica charantia).

PubMed

Tuan, Pham Anh; Park, Sang Un

2013-01-01

Carotenoid cleavage dioxygenases (CCDs) are a family of enzymes that catalyze the oxidative cleavage of carotenoids at various chain positions to form a broad spectrum of apocarotenoids, including aromatic substances, pigments and phytohormones. Using the rapid amplification of cDNA ends (RACE) PCR method, we isolated three cDNA-encoding CCDs (McCCD1, McCCD4, and McNCED) from Momordica charantia. Amino acid sequence alignments showed that they share high sequence identity with other orthologous genes. Quantitative real-time RT PCR (reverse transcriptase PCR) analysis revealed that the expression of McCCD1 and McCCD4 was highest in flowers, and lowest in roots and old leaves (O-leaves). During fruit maturation, the two genes displayed differential expression, with McCCD1 peaking at mid-stage maturation while McCCD4 showed the lowest expression at that stage. The mRNA expression level of McNCED, a key enzyme involved in abscisic acid (ABA) biosynthesis, was high during fruit maturation and further increased at the beginning of seed germination. When first-leaf stage plants of M. charantia were exposed to dehydration stress, McNCED mRNA expression was induced primarily in the leaves and, to a lesser extend, in roots and stems. McNCED expression was also induced by high temperature and salinity, while treatment with exogenous ABA led to a decrease. These results should be helpful in determining the substrates and cleavage sites catalyzed by CCD genes in M. charantia, and also in defining the roles of CCDs in growth and development, and in the plant's response to environmental stress. Copyright © 2012 Elsevier GmbH. All rights reserved.

Paleoenvironments of the Jurassic and Cretaceous Oceans: Selected Highlights

NASA Astrophysics Data System (ADS)

Ogg, J. G.

2007-12-01

There are many themes contributing to the sedimentation history of the Mesozoic oceans. This overview briefly examines the roles of the carbonate compensation depth (CCD) and the associated levels of atmospheric carbon dioxide, of the evolution of marine calcareous microplankton, of major transgressive and regressive trends, and of super-plume eruptions. Initiation of Atlantic seafloor spreading in the Middle Jurassic coincided with an elevated carbonate compensation depth (CCD) in the Pacific-Tethys mega-ocean. Organic-rich sediments that would become the oil wealth of regions from Saudi Arabia to the North Sea were deposited during a continued rise in CCD during the Oxfordian-early Kimmeridgian, which suggests a possible increase in carbon dioxide release by oceanic volcanic activity. Deep-sea deposits in near-equatorial settings are dominated by siliceous shales or cherts, which reflect the productivity of siliceous microfossils in the tropical surface waters. The end-Jurassic explosion in productivity by calcareous microplankton contributed to the lowering of the CCD and onset of the chalk ("creta") deposits that characterize the Tithonian and lower Cretaceous in all ocean basins. During the mid-Cretaceous, the eruption of enormous Pacific igneous provinces (Ontong Java Plateau and coeval edifices) increased carbon dioxide levels. The resulting rise in CCD terminated chalk deposition in the deep sea. The excess carbon was progressively removed in widespread black-shale deposits in the Atlantic basins and other regions - another major episode of oil source rock. A major long-term transgression during middle and late Cretaceous was accompanied by extensive chalk deposition on continental shelves and seaways while the oceanic CCD remained elevated. Pacific guyots document major oscillations (sequences) of global sea level superimposed on this broad highstand. The Cretaceous closed with a progressive sea-level regression and lowering of the CCD that again enabled widespread carbonate deposition in the deep sea.

Transgenic mice expressing mutant Pinin exhibit muscular dystrophy, nebulin deficiency and elevated expression of slow-type muscle fiber genes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, Hsu-Pin; Hsu, Shu-Yuan; Wu, Wen-Ai

Highlights: •Pnn CCD domain functions as a dominant negative mutant regulating Pnn expression and function. •Pnn CCD mutant Tg mice have a muscle wasting phenotype during development and show dystrophic histological features. •Pnn mutant muscles are susceptible to slow fiber type gene transition and NEB reduction. •The Tg mouse generated by overexpression of the Pnn CCD domain displays many characteristics resembling NEB{sup +/−} mice. -- Abstract: Pinin (Pnn) is a nuclear speckle-associated SR-like protein. The N-terminal region of the Pnn protein sequence is highly conserved from mammals to insects, but the C-terminal RS domain-containing region is absent in lower species.more » The N-terminal coiled-coil domain (CCD) is, therefore, of interest not only from a functional point of view, but also from an evolutionarily standpoint. To explore the biological role of the Pnn CCD in a physiological context, we generated transgenic mice overexpressing Pnn mutant in skeletal muscle. We found that overexpression of the CCD reduces endogenous Pnn expression in cultured cell lines as well as in transgenic skeletal muscle fibers. Pnn mutant mice exhibited reduced body mass and impaired muscle function during development. Mutant skeletal muscles show dystrophic histological features with muscle fibers heavily loaded with centrally located myonuclei. Expression profiling and pathway analysis identified over-representation of genes in gene categories associated with muscle contraction, specifically those related to slow type fiber. In addition nebulin (NEB) expression level is repressed in Pnn mutant skeletal muscle. We conclude that Pnn downregulation in skeletal muscle causes a muscular dystrophic phenotype associated with NEB deficiency and the CCD domain is incapable of replacing full length Pnn in terms of functional capacity.« less

Colorectal cancer mortality reduction is associated with having at least 1 colonoscopy within the previous 10 years among a population-wide cohort of screening age.

PubMed

Stock, David; Paszat, Lawrence F; Rabeneck, Linda

2016-07-01

Colonoscopy has been demonstrated to be effective in colorectal cancer (CRC) mortality reduction, although current screening guidelines have yet to be evaluated. We assessed the protective benefit of colonoscopy within the previous 10 years and whether this effect is maintained with age. We used administrative data to compare risk of CRC death (CCD) across colonoscopy utilization among a population-wide cohort comprising individuals aged 60 to 80 years (N = 1,509,423). Baseline and time-dependent colonoscopy exposure models were assessed in the context of competing "other causes of death" (OCDs). Cumulative incidence of CCD and OCD across colonoscopy exposure, over follow-up, was estimated. Relative hazards were computed by age strata (60-69 years, 70-74 years, 75+ years) and proximal and distal cancer subsites. At least 1 colonoscopy during 10 years before baseline was estimated to provide a 51% reduced hazard of CCD (hazard ratio [HR] 0.49; 95% confidence interval [CI], 0.45-0.54) over the following 8 years. When colonoscopy was modeled as a time-dependent covariate, the risk of CCD was further diminished (multivariable-adjusted HR 0.36; 95% CI, 0.33-0.38). Stratified analyses suggested moderately attenuated CCD risk reduction among the oldest age group; however, consideration of OCDs suggest that this is related to competing risks. CCD risk reduction related to colonoscopy was lower for proximal cancers. Colonoscopy within the previous 10 years provides substantial protective benefit for average-risk individuals over 60 years. CCD risk reduction may be maintained well beyond 74 years, a common upper age limit recommended by screening guidelines. Copyright © 2016 American Society for Gastrointestinal Endoscopy. Published by Elsevier Inc. All rights reserved.

Effects of biomechanical forces on signaling in the cortical collecting duct (CCD).

PubMed

Carrisoza-Gaytan, Rolando; Liu, Yu; Flores, Daniel; Else, Cindy; Lee, Heon Goo; Rhodes, George; Sandoval, Ruben M; Kleyman, Thomas R; Lee, Francis Young-In; Molitoris, Bruce; Satlin, Lisa M; Rohatgi, Rajeev

2014-07-15

An increase in tubular fluid flow rate (TFF) stimulates Na reabsorption and K secretion in the cortical collecting duct (CCD) and subjects cells therein to biomechanical forces including fluid shear stress (FSS) and circumferential stretch (CS). Intracellular MAPK and extracellular autocrine/paracrine PGE2 signaling regulate cation transport in the CCD and, at least in other systems, are affected by biomechanical forces. We hypothesized that FSS and CS differentially affect MAPK signaling and PGE2 release to modulate cation transport in the CCD. To validate that CS is a physiological force in vivo, we applied the intravital microscopic approach to rodent kidneys in vivo to show that saline or furosemide injection led to a 46.5 ± 2.0 or 170 ± 32% increase, respectively, in distal tubular diameter. Next, murine CCD (mpkCCD) cells were grown on glass or silicone coated with collagen type IV and subjected to 0 or 0.4 dyne/cm(2) of FSS or 10% CS, respectively, forces chosen based on prior biomechanical modeling of ex vivo microperfused CCDs. Cells exposed to FSS expressed an approximately twofold greater abundance of phospho(p)-ERK and p-p38 vs. static cells, while CS did not alter p-p38 and p-ERK expression compared with unstretched controls. FSS induced whereas CS reduced PGE2 release by ∼40%. In conclusion, FSS and CS differentially affect ERK and p38 activation and PGE2 release in a cell culture model of the CD. We speculate that TFF differentially regulates biomechanical signaling and, in turn, cation transport in the CCD. Copyright © 2014 the American Physiological Society.

Portal imaging with flat-panel detector and CCD camera

NASA Astrophysics Data System (ADS)

Roehrig, Hans; Tang, Chuankun; Cheng, Chee-Wai; Dallas, William J.

1997-07-01

This paper provides a comparison of imaging parameters of two portal imaging systems at 6 MV: a flat panel detector and a CCD-camera based portal imaging system. Measurements were made of the signal and noise and consequently of signal-to-noise per pixel as a function of the exposure. Both systems have a linear response with respect to exposure, and the noise is proportional to the square-root of the exposure, indicating photon-noise limitation. The flat-panel detector has a signal- to-noise ratio, which is higher than that observed wit the CCD-camera based portal imaging system. This is expected because most portal imaging systems using optical coupling with a lens exhibit severe quantum-sinks. The paper also presents data on the screen's photon gain (the number of light-photons per interacting x-ray photon), as well as on the magnitude of the Swank-noise, (which describes fluctuation in the screen's photon gain). Images of a Las Vegas-type aluminum contrast detail phantom, located at the ISO-Center, were generated at an exposure of 1 MU. The CCD-camera based system permits detection of aluminum-holes of 0.01194 cm diameter and 0.228 mm depth while the flat-panel detector permits detection of aluminum holes of 0.01194 cm diameter and 0.1626 mm depth, indicating a better signal-to-noise ratio. Rank order filtering was applied to the raw images from the CCD-based system in order to remove the direct hits. These are camera responses to scattered x-ray photons which interact directly with the CCD of the CCD-camera and generate 'salt and pepper type noise,' which interferes severely with attempts to determine accurate estimates of the image noise.

Wetting morphologies on randomly oriented fibers.

PubMed

Sauret, Alban; Boulogne, François; Soh, Beatrice; Dressaire, Emilie; Stone, Howard A

2015-06-01

We characterize the different morphologies adopted by a drop of liquid placed on two randomly oriented fibers, which is a first step toward understanding the wetting of fibrous networks. The present work reviews previous modeling for parallel and touching crossed fibers and extends it to an arbitrary orientation of the fibers characterized by the tilting angle and the minimum spacing distance. Depending on the volume of liquid, the spacing distance between fibers and the angle between the fibers, we highlight that the liquid can adopt three different equilibrium morphologies: 1) a column morphology in which the liquid spreads between the fibers, 2) a mixed morphology where a drop grows at one end of the column or 3) a single drop located at the node. We capture the different morphologies observed using an analytical model that predicts the equilibrium configuration of the liquid based on the geometry of the fibers and the volume of liquid.

A Simplified Method for Sampling and Analysis of High Volume Surface Water for Organic Contaminants Using XAD-2

USGS Publications Warehouse

Datta, S.; Do, L.V.; Young, T.M.

2004-01-01

A simple compressed-gas driven system for field processing and extracting water for subsequent analyses of hydrophobic organic compounds is presented. The pumping device is a pneumatically driven pump and filtration system that can easily clarify at 4L/min. The extraction device uses compressed gas to drive filtered water through two parallel XAD-2 resin columns, at about 200 mL/min. No batteries or inverters are required for water collection or processing. Solvent extractions were performed directly in the XAD-2 glass columns. Final extracts are cleaned-up on Florisil cartridges without fractionation and contaminants analyzed by GC-MS. Method detection limits (MDLs) and recoveries for dissolved organic contaminants, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and pesticides are reported along with results of surface water analysis for the San Francisco Bay, CA.

Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and microRPLC-ESI-MS/MS.

PubMed

Yuan, Huiming; Zhou, Yuan; Zhang, Lihua; Liang, Zhen; Zhang, Yukui

2009-10-30

An integrated platform with the combination of proteins and peptides separation was established via the unit of on-line proteins digestion, by which proteins were in sequence separated by column switch recycling size exclusion chromatography (csrSEC), on-line digested by an immobilized trypsin microreactor, trapped and desalted by two parallel C8 precolumns, separated by microRPLC with the linear gradient of organic modifier concentration, and identified by ESI-MS/MS. A 6-protein mixture, with Mr ranging from 10 kDa to 80 kDa, was used to evaluate the performance of the integrated platform, and all proteins were identified with sequence coverage over 5.67%. Our experimental results demonstrate that such an integrated platform is of advantages such as good time compatibility, high peak capacity, and facile automation, which might be a promising approach for proteome study.

Indoor air quality analysis based on Hadoop

NASA Astrophysics Data System (ADS)

Tuo, Wang; Yunhua, Sun; Song, Tian; Liang, Yu; Weihong, Cui

2014-03-01

The air of the office environment is our research object. The data of temperature, humidity, concentrations of carbon dioxide, carbon monoxide and ammonia are collected peer one to eight seconds by the sensor monitoring system. And all the data are stored in the Hbase database of Hadoop platform. With the help of HBase feature of column-oriented store and versioned (automatically add the time column), the time-series data sets are bulit based on the primary key Row-key and timestamp. The parallel computing programming model MapReduce is used to process millions of data collected by sensors. By analysing the changing trend of parameters' value at different time of the same day and at the same time of various dates, the impact of human factor and other factors on the room microenvironment is achieved according to the liquidity of the office staff. Moreover, the effective way to improve indoor air quality is proposed in the end of this paper.

CTK: A new CCD Camera at the University Observatory Jena

NASA Astrophysics Data System (ADS)

Mugrauer, M.

2009-05-01

The Cassegrain-Teleskop-Kamera (CTK) is a new CCD imager which is operated at the University Observatory Jena since begin of 2006. This article describes the main characteristics of the new camera. The properties of the CCD detector, the CTK image quality, as well as its detection limits for all filters are presented. Based on observations obtained with telescopes of the University Observatory Jena, which is operated by the Astrophysical Institute of the Friedrich-Schiller-University.

Astronomical Archive at Tartu Observatory

NASA Astrophysics Data System (ADS)

Annuk, K.

2007-10-01

Archiving astronomical data is important task not only at large observatories but also at small observatories. Here we describe the astronomical archive at Tartu Observatory. The archive consists of old photographic plate images, photographic spectrograms, CCD direct--images and CCD spectroscopic data. The photographic plate digitizing project was started in 2005. An on-line database (based on MySQL) was created. The database includes CCD data as well photographic data. A PHP-MySQL interface was written for access to all data.

Evaluation of tellurium toxicity in transformed and non-transformed human colon cells.

PubMed

Vij, Puneet; Hardej, Diane

2012-11-01

Diphenyl ditelluride (DPDT) and tellurium tetrachloride (TeCl(4)) were evaluated for toxicity in transformed (HT-29, Caco-2) and non-transformed colon cells (CCD-18Co). Significant decreases in viability were observed with DPDT exposure in HT-29 (62.5-1000 μM), Caco-2 (31.25-1000 μM) and CCD-18Co cells (500-1000 μM) and with TeCl(4) in HT-29 (31.25-1000 μM), Caco-2 (31.25-1000 μM) and CCD-18Co cells (500-1000 μM). Light microscopy confirmed viability analysis. Significant increases in caspase 3/7 and 9 activity were observed with DPDT in HT-29 (500-1000 μM) and CCD-18Co cells (1000 μM) indicating apoptosis. No significant increases in caspases were seen with TeCl(4) indicating necrosis. Apoptosis or necrosis was confirmed with fluorescent staining (FITC-Annexin, Hoechst 33342 and Ethidium Homodimer). Significant decreases in GSH/GSSG ratio were observed with DPDT in HT-29 (62.5-1000 μM), and CCD-18Co cells (1000 μM) and with TeCl(4) in HT-29 (62.5-1000 μM) and CCD-18Co cells (250-1000 μM). We concluded that cells treated with DPDT resulted in apoptosis and TeCl(4) treatment in necrosis. GSH/GSSG ratio shifts indicate oxidative mechanisms are involved. Copyright © 2012 Elsevier B.V. All rights reserved.

LED characterization for development of on-board calibration unit of CCD-based advanced wide-field sensor camera of Resourcesat-2A

NASA Astrophysics Data System (ADS)

Chatterjee, Abhijit; Verma, Anurag

2016-05-01

The Advanced Wide Field Sensor (AWiFS) camera caters to high temporal resolution requirement of Resourcesat-2A mission with repeativity of 5 days. The AWiFS camera consists of four spectral bands, three in the visible and near IR and one in the short wave infrared. The imaging concept in VNIR bands is based on push broom scanning that uses linear array silicon charge coupled device (CCD) based Focal Plane Array (FPA). On-Board Calibration unit for these CCD based FPAs is used to monitor any degradation in FPA during entire mission life. Four LEDs are operated in constant current mode and 16 different light intensity levels are generated by electronically changing exposure of CCD throughout the calibration cycle. This paper describes experimental setup and characterization results of various flight model visible LEDs (λP=650nm) for development of On-Board Calibration unit of Advanced Wide Field Sensor (AWiFS) camera of RESOURCESAT-2A. Various LED configurations have been studied to meet dynamic range coverage of 6000 pixels silicon CCD based focal plane array from 20% to 60% of saturation during night pass of the satellite to identify degradation of detector elements. The paper also explains comparison of simulation and experimental results of CCD output profile at different LED combinations in constant current mode.

Differential spatio-temporal expression of carotenoid cleavage dioxygenases regulates apocarotenoid fluxes during AM symbiosis.

PubMed

López-Ráez, Juan A; Fernández, Iván; García, Juan M; Berrio, Estefanía; Bonfante, Paola; Walter, Michael H; Pozo, María J

2015-01-01

Apocarotenoids are a class of compounds that play important roles in nature. In recent years, a prominent role for these compounds in arbuscular mycorrhizal (AM) symbiosis has been shown. They are derived from carotenoids by the action of the carotenoid cleavage dioxygenase (CCD) enzyme family. In the present study, using tomato as a model, the spatio-temporal expression pattern of the CCD genes during AM symbiosis establishment and functioning was investigated. In addition, the levels of the apocarotenoids strigolactones (SLs), C13 α-ionol and C14 mycorradicin (C13/C14) derivatives were analyzed. The results suggest an increase in SLs promoted by the presence of the AM fungus at the early stages of the interaction, which correlated with an induction of the SL biosynthesis gene SlCCD7. At later stages, induction of SlCCD7 and SlCCD1 expression in arbusculated cells promoted the production of C13/C14 apocarotenoid derivatives. We show here that the biosynthesis of apocarotenoids during AM symbiosis is finely regulated throughout the entire process at the gene expression level, and that CCD7 constitutes a key player in this regulation. Once the symbiosis is established, apocarotenoid flux would be turned towards the production of C13/C14 derivatives, thus reducing SL biosynthesis and maintaining a functional symbiosis. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

[Application of small remote sensing satellite constellations for environmental hazards in wetland landscape mapping: taking Liaohe Delta, Liaoning Province of Northeast China as a case].

PubMed

Yang, Yuan-Zheng; Chang, Yu; Hu, Yuan-Man; Liu, Miao; Li, Yue-Hui

2011-06-01

To timely and accurately acquire the spatial distribution pattern of wetlands is of significance for the dynamic monitoring, conservation, and sustainable utilization of wetlands. The small remote sensing satellite constellations A/B stars (HJ-1A/1B stars) for environmental hazards were launched by China for monitoring terrestrial resources, which could provide a new data source of remote sensing image acquisition for retrieving wetland types. Taking Liaohe Delta as a case, this paper compared the accuracy of wetland classification map and the area of each wetland type retrieved from CCD data (HJ CCD data) and TM5 data, and validated and explored the applicability and the applied potential of HJ CCD data in wetland resources dynamic monitoring. The results showed that HJ CCD data could completely replace Landsat TM5 data in feature extraction and remote sensing classification. In real-time monitoring, due to its 2 days of data acquisition cycle, HJ CCD data had the priority to Landsat TM5 data (16 days of data acquisition cycle).

Replacing a technology - The Large Space Telescope and CCDs

NASA Astrophysics Data System (ADS)

Smith, R. W.; Tatarewicz, J. H.

1985-07-01

The technological improvements, design choices and mission goals which led to the inclusion of CCD detectors in the wide field camera of the Large Space Telescope (LST) to be launched by the STS are recounted. Consideration of CCD detectors began before CCDs had seen wide astronomical applications. During planning for the ST, in the 1960s, photographic methods and a vidicon were considered, and seemed feasible provided that periodic manual maintenance could be performed. The invention of CCDs was first reported in 1970 and by 1973 the CCDs were receiving significant attention as potential detectors instead of a vidicon, which retained its own technological challenges. The CCD format gained new emphasis when success was achieved in developments for planetary-imaging spacecraft. The rapidity of progress in CCD capabilities, coupled with the continued shortcomings of the vidicon, resulted in a finalized choice for a CCD device by 1977. The decision was also prompted by continuing commercial and military interest in CCDs, which was spurring the development of the technology and improving the sensitivities and reliability while lowering the costs.

Final Report, January 1991 - July 1992

NASA Astrophysics Data System (ADS)

Ferrara, Jon

1992-07-01

This report covers final schedules, expenses and billings, monthly reports, testing, and deliveries for this contract. The goal of the detector development program for the Solar and Heliospheric Spacecraft (SOHO) EUV Imaging Telescope (EIT) is an Extreme UltraViolet (EUV) CCD (Change Collecting Device) camera. As a part of the CCD screening effort, the quantum efficiency (QE) of a prototype CCD has been measured in the NRL EUV laboratory over the wavelength range of 256 to 735 Angstroms. A simplified model has been applied to these QE measurements to illustrate the relevant physical processes that determine the performance of the detector. The charge transfer efficiency (CTE) characteristics of the Tektronix 1024 X 1024 CCD being developed for STIS/SOHO space imaging applications have been characterized at different signal levels, operating conditions, and temperatures using a variety of test methods. A number of CCD's have been manufactured using processing techniques developed to improve CTE, and test results on these devices will be used in determining the final chip design. In this paper, we discuss the CTE test methods used and present the results and conclusions of these tests.

The chemical component dictionary: complete descriptions of constituent molecules in experimentally determined 3D macromolecules in the Protein Data Bank

PubMed Central

Westbrook, John D.; Shao, Chenghua; Feng, Zukang; Zhuravleva, Marina; Velankar, Sameer; Young, Jasmine

2015-01-01

Summary: The Chemical Component Dictionary (CCD) is a chemical reference data resource that describes all residue and small molecule components found in Protein Data Bank (PDB) entries. The CCD contains detailed chemical descriptions for standard and modified amino acids/nucleotides, small molecule ligands and solvent molecules. Each chemical definition includes descriptions of chemical properties such as stereochemical assignments, chemical descriptors, systematic chemical names and idealized coordinates. The content, preparation, validation and distribution of this CCD chemical reference dataset are described. Availability and implementation: The CCD is updated regularly in conjunction with the scheduled weekly release of new PDB structure data. The CCD and amino acid variant reference datasets are hosted in the public PDB ftp repository at ftp://ftp.wwpdb.org/pub/pdb/data/monomers/components.cif.gz, ftp://ftp.wwpdb.org/pub/pdb/data/monomers/aa-variants-v1.cif.gz, and its mirror sites, and can be accessed from http://wwpdb.org. Contact: jwest@rcsb.rutgers.edu. Supplementary information: Supplementary data are available at Bioinformatics online. PMID:25540181

Quantum efficiency measurement of the Transiting Exoplanet Survey Satellite (TESS) CCD detectors

NASA Astrophysics Data System (ADS)

Krishnamurthy, A.; Villasenor, J.; Thayer, C.; Kissel, S.; Ricker, G.; Seager, S.; Lyle, R.; Deline, A.; Morgan, E.; Sauerwein, T.; Vanderspek, R.

2016-07-01

Very precise on-ground characterization and calibration of TESS CCD detectors will significantly assist in the analysis of the science data from the mission. An accurate optical test bench with very high photometric stability has been developed to perform precise measurements of the absolute quantum efficiency. The setup consists of a vacuum dewar with a single MIT Lincoln Lab CCID-80 device mounted on a cold plate with the calibrated reference photodiode mounted next to the CCD. A very stable laser-driven light source is integrated with a closed-loop intensity stabilization unit to control variations of the light source down to a few parts-per-million when averaged over 60 s. Light from the stabilization unit enters a 20 inch integrating sphere. The output light from the sphere produces near-uniform illumination on the cold CCD and on the calibrated reference photodiode inside the dewar. The ratio of the CCD and photodiode signals provides the absolute quantum efficiency measurement. The design, key features, error analysis, and results from the test campaign are presented.

Improvement in the light sensitivity of the ultrahigh-speed high-sensitivity CCD with a microlens array

NASA Astrophysics Data System (ADS)

Hayashida, T.,; Yonai, J.; Kitamura, K.; Arai, T.; Kurita, T.; Tanioka, K.; Maruyama, H.; Etoh, T. Goji; Kitagawa, S.; Hatade, K.; Yamaguchi, T.; Takeuchi, H.; Iida, K.

2008-02-01

We are advancing the development of ultrahigh-speed, high-sensitivity CCDs for broadcast use that are capable of capturing smooth slow-motion videos in vivid colors even where lighting is limited, such as at professional baseball games played at night. We have already developed a 300,000 pixel, ultrahigh-speed CCD, and a single CCD color camera that has been used for sports broadcasts and science programs using this CCD. However, there are cases where even higher sensitivity is required, such as when using a telephoto lens during a baseball broadcast or a high-magnification microscope during science programs. This paper provides a summary of our experimental development aimed at further increasing the sensitivity of CCDs using the light-collecting effects of a microlens array.

Tests of commercial colour CMOS cameras for astronomical applications

NASA Astrophysics Data System (ADS)

Pokhvala, S. M.; Reshetnyk, V. M.; Zhilyaev, B. E.

2013-12-01

We present some results of testing commercial colour CMOS cameras for astronomical applications. Colour CMOS sensors allow to perform photometry in three filters simultaneously that gives a great advantage compared with monochrome CCD detectors. The Bayer BGR colour system realized in colour CMOS sensors is close to the astronomical Johnson BVR system. The basic camera characteristics: read noise (e^{-}/pix), thermal noise (e^{-}/pix/sec) and electronic gain (e^{-}/ADU) for the commercial digital camera Canon 5D MarkIII are presented. We give the same characteristics for the scientific high performance cooled CCD camera system ALTA E47. Comparing results for tests of Canon 5D MarkIII and CCD ALTA E47 show that present-day commercial colour CMOS cameras can seriously compete with the scientific CCD cameras in deep astronomical imaging.

Ion-molecule calculation of the abundance ratio of CCD to CCH in dense interstellar clouds

NASA Technical Reports Server (NTRS)

Herbst, Eric; Adams, Nigel G.; Smith, David; Defrees, D. J.

1987-01-01

Laboratory measurements and calculations have been performed to determine the abundance ratio of the deuterated ethynyl radical (CCD) to the normal radical (CCH) which can be achieved in dense interstellar clouds via isotopic fractionation in the C2H2(+) (HD)=C2HD(+)(H2) system of reactions. According to this limited treatment, the CCD/CCH abundance ratio which can be attained is in the range 0.02-0.03 for the Orion molecular cloud and 0.0l-0.02 for TMC-1. These ranges of numbers are in reasonable agreement with the observed values in Orion and TMC-1. However, the analysis of the CCD/CCH abundance ratio is complicated via the presence of competing fractionation mechanisms, especially in the low-temperature source TMC-1.

Solid state television camera (CCD-buried channel)

NASA Technical Reports Server (NTRS)

1976-01-01

The development of an all solid state television camera, which uses a buried channel charge coupled device (CCD) as the image sensor, was undertaken. A 380 x 488 element CCD array is utilized to ensure compatibility with 525 line transmission and display monitor equipment. Specific camera design approaches selected for study and analysis included (a) optional clocking modes for either fast (1/60 second) or normal (1/30 second) frame readout, (b) techniques for the elimination or suppression of CCD blemish effects, and (c) automatic light control and video gain control (i.e., ALC and AGC) techniques to eliminate or minimize sensor overload due to bright objects in the scene. Preferred approaches were determined and integrated into a deliverable solid state TV camera which addressed the program requirements for a prototype qualifiable to space environment conditions.

Solid state television camera (CCD-buried channel), revision 1

NASA Technical Reports Server (NTRS)

1977-01-01

An all solid state television camera was designed which uses a buried channel charge coupled device (CCD) as the image sensor. A 380 x 488 element CCD array is utilized to ensure compatibility with 525-line transmission and display monitor equipment. Specific camera design approaches selected for study and analysis included (1) optional clocking modes for either fast (1/60 second) or normal (1/30 second) frame readout, (2) techniques for the elimination or suppression of CCD blemish effects, and (3) automatic light control and video gain control techniques to eliminate or minimize sensor overload due to bright objects in the scene. Preferred approaches were determined and integrated into a deliverable solid state TV camera which addressed the program requirements for a prototype qualifiable to space environment conditions.

Solid state, CCD-buried channel, television camera study and design

NASA Technical Reports Server (NTRS)

Hoagland, K. A.; Balopole, H.

1976-01-01

An investigation of an all solid state television camera design, which uses a buried channel charge-coupled device (CCD) as the image sensor, was undertaken. A 380 x 488 element CCD array was utilized to ensure compatibility with 525 line transmission and display monitor equipment. Specific camera design approaches selected for study and analysis included (a) optional clocking modes for either fast (1/60 second) or normal (1/30 second) frame readout, (b) techniques for the elimination or suppression of CCD blemish effects, and (c) automatic light control and video gain control techniques to eliminate or minimize sensor overload due to bright objects in the scene. Preferred approaches were determined and integrated into a design which addresses the program requirements for a deliverable solid state TV camera.

MAPPER: high-throughput maskless lithography

NASA Astrophysics Data System (ADS)

Wieland, M. J.; de Boer, G.; ten Berge, G. F.; Jager, R.; van de Peut, T.; Peijster, J. J. M.; Slot, E.; Steenbrink, S. W. H. K.; Teepen, T. F.; van Veen, A. H. V.; Kampherbeek, B. J.

2009-03-01

Maskless electron beam lithography, or electron beam direct write, has been around for a long time in the semiconductor industry and was pioneered from the mid-1960s onwards. This technique has been used for mask writing applications as well as device engineering and in some cases chip manufacturing. However because of its relatively low throughput compared to optical lithography, electron beam lithography has never been the mainstream lithography technology. To extend optical lithography double patterning, as a bridging technology, and EUV lithography are currently explored. Irrespective of the technical viability of both approaches, one thing seems clear. They will be expensive [1]. MAPPER Lithography is developing a maskless lithography technology based on massively-parallel electron-beam writing with high speed optical data transport for switching the electron beams. In this way optical columns can be made with a throughput of 10-20 wafers per hour. By clustering several of these columns together high throughputs can be realized in a small footprint. This enables a highly cost-competitive alternative to double patterning and EUV alternatives. In 2007 MAPPER obtained its Proof of Lithography milestone by exposing in its Demonstrator 45 nm half pitch structures with 110 electron beams in parallel, where all the beams where individually switched on and off [2]. In 2008 MAPPER has taken a next step in its development by building several tools. The objective of building these tools is to involve semiconductor companies to be able to verify tool performance in their own environment. To enable this, the tools will have a 300 mm wafer stage in addition to a 110-beam optics column. First exposures at 45 nm half pitch resolution have been performed and analyzed. On the same wafer it is observed that all beams print and based on analysis of 11 beams the CD for the different patterns is within 2.2 nm from target and the CD uniformity for the different patterns is better than 2.8 nm.

Archeological and Historical Studies in the White Castle Gap Revetment, Iberville Parish, Louisiana.

DTIC Science & Technology

1982-01-01

enigmatic nature of the Poverty Point type site, coupled with its temporal parallels in coastal Mexico , lead Weaver (1972: 281) to suggest that the horizon...trade between Mexico and the Mississippi drainage, it must be noted that we have neither evidence of site-unit intrusions of Mexican peoples, nor...Two canoes have examined the coast thirty leagues towards Mexico , and twenty-five towards Florida (deTonty 1905: 35,36). The column to which de Tonty

Multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis

DOEpatents

Karger, Barry L.; Kotler, Lev; Foret, Frantisek; Minarik, Marek; Kleparnik, Karel

2003-12-09

A modular multiple lane or capillary electrophoresis (chromatography) system that permits automated parallel separation and comprehensive collection of all fractions from samples in all lanes or columns, with the option of further on-line automated sample fraction analysis, is disclosed. Preferably, fractions are collected in a multi-well fraction collection unit, or plate (40). The multi-well collection plate (40) is preferably made of a solvent permeable gel, most preferably a hydrophilic, polymeric gel such as agarose or cross-linked polyacrylamide.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Milkov, Mihail M.

A comparator circuit suitable for use in a column-parallel single-slope analog-to-digital converter comprises a comparator, an input voltage sampling switch, a sampling capacitor arranged to store a voltage which varies with an input voltage when the sampling switch is closed, and a local ramp buffer arranged to buffer a global voltage ramp applied at an input. The comparator circuit is arranged such that its output toggles when the buffered global voltage ramp exceeds the stored voltage. Both DC- and AC-coupled comparator embodiments are disclosed.

Interdigitated Color- and Disparity-Selective Columns within Human Visual Cortical Areas V2 and V3

PubMed Central

Polimeni, Jonathan R.; Tootell, Roger B.H.

2016-01-01

In nonhuman primates (NHPs), secondary visual cortex (V2) is composed of repeating columnar stripes, which are evident in histological variations of cytochrome oxidase (CO) levels. Distinctive “thin” and “thick” stripes of dark CO staining reportedly respond selectively to stimulus variations in color and binocular disparity, respectively. Here, we first tested whether similar color-selective or disparity-selective stripes exist in human V2. If so, available evidence predicts that such stripes should (1) radiate “outward” from the V1–V2 border, (2) interdigitate, (3) differ from each other in both thickness and length, (4) be spaced ∼3.5–4 mm apart (center-to-center), and, perhaps, (5) have segregated functional connections. Second, we tested whether analogous segregated columns exist in a “next-higher” tier area, V3. To answer these questions, we used high-resolution fMRI (1 × 1 × 1 mm3) at high field (7 T), presenting color-selective or disparity-selective stimuli, plus extensive signal averaging across multiple scan sessions and cortical surface-based analysis. All hypotheses were confirmed. V2 stripes and V3 columns were reliably localized in all subjects. The two stripe/column types were largely interdigitated (e.g., nonoverlapping) in both V2 and V3. Color-selective stripes differed from disparity-selective stripes in both width (thickness) and length. Analysis of resting-state functional connections (eyes closed) showed a stronger correlation between functionally alike (compared with functionally unlike) stripes/columns in V2 and V3. These results revealed a fine-scale segregation of color-selective or disparity-selective streams within human areas V2 and V3. Together with prior evidence from NHPs, this suggests that two parallel processing streams extend from visual subcortical regions through V1, V2, and V3. SIGNIFICANCE STATEMENT In current textbooks and reviews, diagrams of cortical visual processing highlight two distinct neural-processing streams within the first and second cortical areas in monkeys. Two major streams consist of segregated cortical columns that are selectively activated by either color or ocular interactions. Because such cortical columns are so small, they were not revealed previously by conventional imaging techniques in humans. Here we demonstrate that such segregated columnar systems exist in humans. We find that, in humans, color versus binocular disparity columns extend one full area further, into the third visual area. Our approach can be extended to reveal and study additional types of columns in human cortex, perhaps including columns underlying more cognitive functions. PMID:26865609

An Overview of the CBERS-2 Satellite and Comparison of the CBERS-2 CCD Data with the L5 TM Data

NASA Technical Reports Server (NTRS)

Chandler, Gyanesh

2007-01-01

CBERS satellite carries on-board a multi sensor payload with different spatial resolutions and collection frequencies. HRCCD (High Resolution CCD Camera), IRMSS (Infrared Multispectral Scanner), and WFI (Wide-Field Imager). The CCD and the WFI camera operate in the VNIR regions, while the IRMSS operates in SWIR and thermal region. In addition to the imaging payload, the satellite carries a Data Collection System (DCS) and Space Environment Monitor (SEM).

Design of a CCD Camera for Space Surveillance

DTIC Science & Technology

2016-03-05

Laboratory fabricated CCID-51M, a 2048x1024 pixel Charge Couple Device (CCD) imager. [1] The mission objective is to observe and detect satellites in...phased to transfer the charge to the outputs. An electronic shutter is created by having an equal area of pixels covered by an opaque metal mask. The...Figure 4 CDS Timing Diagram By design the CCD readout rate is 400 KHz. This rate was chosen so reading the 2E6 pixels from one output is less than

Registering coherent change detection products associated with large image sets and long capture intervals

DOEpatents

Perkins, David Nikolaus; Gonzales, Antonio I

2014-04-08

A set of co-registered coherent change detection (CCD) products is produced from a set of temporally separated synthetic aperture radar (SAR) images of a target scene. A plurality of transformations are determined, which transformations are respectively for transforming a plurality of the SAR images to a predetermined image coordinate system. The transformations are used to create, from a set of CCD products produced from the set of SAR images, a corresponding set of co-registered CCD products.

CCD Photometer Installed on the Telescope - 600 OF the Shamakhy Astrophysical Observatory: I. Adjustment of CCD Photometer with Optics - 600

NASA Astrophysics Data System (ADS)

Lyuty, V. M.; Abdullayev, B. I.; Alekberov, I. A.; Gulmaliyev, N. I.; Mikayilov, Kh. M.; Rustamov, B. N.

2009-12-01

Short description of optical and electric scheme of CCD photometer with camera U-47 installed on the Cassegrain focus of ZEISS-600 telescope of the ShAO NAS Azerbaijan is provided. The reducer of focus with factor of reduction 1.7 is applied. It is calculated equivalent focal distances of a telescope with a focus reducer. General calculations of optimum distance from focal plane and t sizes of optical filters of photometer are presented.

Development of CCD imaging sensors for space applications, phase 1

NASA Technical Reports Server (NTRS)

Antcliffe, G. A.

1975-01-01

The results of an experimental investigation to develop a large area charge coupled device (CCD) imager for space photography applications are described. Details of the design and processing required to achieve 400 X 400 imagers are presented together with a discussion of the optical characterization techniques developed for this program. A discussion of several aspects of large CCD performance is given with detailed test reports. The areas covered include dark current, uniformity of optical response, square wave amplitude response, spectral responsivity and dynamic range.

Design of a Low-Light-Level Image Sensor with On-Chip Sigma-Delta Analog-to- Digital Conversion

NASA Technical Reports Server (NTRS)

Mendis, Sunetra K.; Pain, Bedabrata; Nixon, Robert H.; Fossum, Eric R.

1993-01-01

The design and projected performance of a low-light-level active-pixel-sensor (APS) chip with semi-parallel analog-to-digital (A/D) conversion is presented. The individual elements have been fabricated and tested using MOSIS* 2 micrometer CMOS technology, although the integrated system has not yet been fabricated. The imager consists of a 128 x 128 array of active pixels at a 50 micrometer pitch. Each column of pixels shares a 10-bit A/D converter based on first-order oversampled sigma-delta (Sigma-Delta) modulation. The 10-bit outputs of each converter are multiplexed and read out through a single set of outputs. A semi-parallel architecture is chosen to achieve 30 frames/second operation even at low light levels. The sensor is designed for less than 12 e^- rms noise performance.

Ion heating and flows in a high power helicon source

NASA Astrophysics Data System (ADS)

Thompson, Derek S.; Agnello, Riccardo; Furno, Ivo; Howling, Alan; Jacquier, Rémy; Plyushchev, Gennady; Scime, Earl E.

2017-06-01

We report experimental measurements of ion temperatures and flows in a high power, linear, magnetized, helicon plasma device, the Resonant Antenna Ion Device (RAID). Parallel and perpendicular ion temperatures on the order of 0.6 eV are observed for an rf power of 4 kW, suggesting that higher power helicon sources should attain ion temperatures in excess of 1 eV. The unique RAID antenna design produces broad, uniform plasma density and perpendicular ion temperature radial profiles. Measurements of the azimuthal flow indicate rigid body rotation of the plasma column of a few kHz. When configured with an expanding magnetic field, modest parallel ion flows are observed in the expansion region. The ion flows and temperatures are derived from laser induced fluorescence measurements of the Doppler resolved velocity distribution functions of argon ions.

Treatment of groundwater containing Mn(II), Fe(II), As(III) and Sb(III) by bioaugmented quartz-sand filters.

PubMed

Bai, Yaohui; Chang, Yangyang; Liang, Jinsong; Chen, Chen; Qu, Jiuhui

2016-12-01

High concentrations of iron (Fe(II)) and manganese (Mn(II)) often occur simultaneously in groundwater. Previously, we demonstrated that Fe(II) and Mn(II) could be oxidized to biogenic Fe-Mn oxides (BFMO) via aeration and microbial oxidation, and the formed BFMO could further oxidize and adsorb other pollutants (e.g., arsenic (As(III)) and antimony (Sb(III))). To apply this finding to groundwater remediation, we established four quartz-sand columns for treating groundwater containing Fe(II), Mn(II), As(III), and Sb(III). A Mn-oxidizing bacterium (Pseudomonas sp. QJX-1) was inoculated into two parallel bioaugmented columns. Long-term treatment (120 d) showed that bioaugmentation accelerated the formation of Fe-Mn oxides, resulting in an increase in As and Sb removal. The bioaugmented columns also exhibited higher overall treatment effect and anti-shock load capacity than that of the non-bioaugmented columns. To clarify the causal relationship between the microbial community and treatment effect, we compared the biomass of active bacteria (reverse-transcribed real-time PCR), bacterial community composition (Miseq 16S rRNA sequencing) and community function (metagenomic sequencing) between the bioaugmented and non-bioaugmented columns. Results indicated that the QJX1 strain grew steadily and attached onto the filter material surface in the bioaugmented columns. In general, the inoculated strain did not significantly alter the composition of the indigenous bacterial community, but did improve the relative abundances of xenobiotic metabolism genes and Mn oxidation gene. Thus, bioaugmentation intensified microbial degradation/utilization for the direct removal of pollutants and increased the formation of Fe-Mn oxides for the indirect removal of pollutants. Our study provides an alternative method for the treatment of groundwater containing high Fe(II), Mn(II) and As/Sb. Copyright © 2016 Elsevier Ltd. All rights reserved.

Calibration and evaluation of CCD spectroradiometers for ground-based and airborne measurements of spectral actinic flux densities

NASA Astrophysics Data System (ADS)

Bohn, Birger; Lohse, Insa

2017-09-01

The properties and performance of charge-coupled device (CCD) array spectroradiometers for the measurement of atmospheric spectral actinic flux densities (280-650 nm) and photolysis frequencies were investigated. These instruments are widely used in atmospheric research and are suitable for aircraft applications because of high time resolutions and high sensitivities in the UV range. The laboratory characterization included instrument-specific properties like the wavelength accuracy, dark signal, dark noise and signal-to-noise ratio (SNR). Spectral sensitivities were derived from measurements with spectral irradiance standards. The calibration procedure is described in detail, and a straightforward method to minimize the influence of stray light on spectral sensitivities is introduced. From instrument dark noise, minimum detection limits ≈ 1 × 1010 cm-2 s-1 nm-1 were derived for spectral actinic flux densities at wavelengths around 300 nm (1 s integration time). As a prerequisite for the determination of stray light under field conditions, atmospheric cutoff wavelengths were defined using radiative transfer calculations as a function of the solar zenith angle (SZA) and total ozone column (TOC). The recommended analysis of field data relies on these cutoff wavelengths and is also described in detail taking data from a research flight on HALO (High Altitude and Long Range Research Aircraft) as an example. An evaluation of field data was performed by ground-based comparisons with a double-monochromator-based, highly sensitive reference spectroradiometer. Spectral actinic flux densities were compared as well as photolysis frequencies j(NO2) and j(O1D), representing UV-A and UV-B ranges, respectively. The spectra expectedly revealed increased daytime levels of stray-light-induced signals and noise below atmospheric cutoff wavelengths. The influence of instrument noise and stray-light-induced noise was found to be insignificant for j(NO2) and rather limited for j(O1D), resulting in estimated detection limits of 5 × 10-7 and 1 × 10-7 s-1, respectively, derived from nighttime measurements on the ground (0.3 s integration time, 10 s averages). For j(O1D) the detection limit could be further reduced by setting spectral actinic flux densities to zero below atmospheric cutoff wavelengths. The accuracies of photolysis frequencies were determined from linear regressions with data from the double-monochromator reference instrument. The agreement was typically within ±5 %. Because optical-receiver aspects are not specific for the CCD spectroradiometers, they were widely excluded in this work and will be treated in a separate paper, in particular with regard to airborne applications.

MPI parallelization of Vlasov codes for the simulation of nonlinear laser-plasma interactions

NASA Astrophysics Data System (ADS)

Savchenko, V.; Won, K.; Afeyan, B.; Decyk, V.; Albrecht-Marc, M.; Ghizzo, A.; Bertrand, P.

2003-10-01

The simulation of optical mixing driven KEEN waves [1] and electron plasma waves [1] in laser-produced plasmas require nonlinear kinetic models and massive parallelization. We use Massage Passing Interface (MPI) libraries and Appleseed [2] to solve the Vlasov Poisson system of equations on an 8 node dual processor MAC G4 cluster. We use the semi-Lagrangian time splitting method [3]. It requires only row-column exchanges in the global data redistribution, minimizing the total number of communications between processors. Recurrent communication patterns for 2D FFTs involves global transposition. In the Vlasov-Maxwell case, we use splitting into two 1D spatial advections and a 2D momentum advection [4]. Discretized momentum advection equations have a double loop structure with the outer index being assigned to different processors. We adhere to a code structure with separate routines for calculations and data management for parallel computations. [1] B. Afeyan et al., IFSA 2003 Conference Proceedings, Monterey, CA [2] V. K. Decyk, Computers in Physics, 7, 418 (1993) [3] Sonnendrucker et al., JCP 149, 201 (1998) [4] Begue et al., JCP 151, 458 (1999)

Fast, simple and efficient salting-out assisted liquid-liquid extraction of naringenin from fruit juice samples prior to their enantioselective determination by liquid chromatography.

PubMed

Magiera, Sylwia; Kwietniowska, Ewelina

2016-11-15

In this study, an easy, simple and efficient method for the determination of naringenin enantiomers in fruit juices after salting-out-assisted liquid-liquid extraction (SALLE) and high-performance liquid chromatography (HPLC) with diode-array detection (DAD) was developed. The sample treatment is based on the use of water-miscible acetonitrile as the extractant and acetonitrile phase separation under high-salt conditions. After extraction, juice samples were incubated with hydrochloric acid in order to achieve hydrolysis of naringin to naringenin. The hydrolysis parameters were optimized by using a half-fraction factorial central composite design (CCD). After sample preparation, chromatographic separation was obtained on a Chiralcel® OJ-RH column using the mobile phase consisting of 10mM aqueous ammonium acetate:methanol:acetonitrile (50:30:20; v/v/v) with detection at 288nm. The average recovery of the analyzed compounds ranged from 85.6 to 97.1%. The proposed method was satisfactorily used for the determination of naringenin enantiomers in various fruit juices samples. Copyright © 2016 Elsevier Ltd. All rights reserved.

Development of experimental design approach and ANN-based models for determination of Cr(VI) ions uptake rate from aqueous solution onto the solid biodiesel waste residue.

PubMed

Shanmugaprakash, M; Sivakumar, V

2013-11-01

In the present work, the evaluation capacities of two optimization methodologies such as RSM and ANN were employed and compared for predication of Cr(VI) uptake rate using defatted pongamia oil cake (DPOC) in both batch and column mode. The influence of operating parameters was investigated through a central composite design (CCD) of RSM using Design Expert 8.0.7.1 software. The same data was fed as input in ANN to obtain a trained the multilayer feed-forward networks back-propagation algorithm using MATLAB. The performance of the developed ANN models were compared with RSM mathematical models for Cr(VI) uptake rate in terms of the coefficient of determination (R(2)), root mean square error (RMSE) and absolute average deviation (AAD). The estimated values confirm that ANN predominates RSM representing the superiority of a trained ANN models over RSM models in order to capture the non-linear behavior of the given system. Copyright © 2013 Elsevier Ltd. All rights reserved.

Experimental research on femto-second laser damaging array CCD cameras

NASA Astrophysics Data System (ADS)

Shao, Junfeng; Guo, Jin; Wang, Ting-feng; Wang, Ming

2013-05-01

Charged Coupled Devices (CCD) are widely used in military and security applications, such as airborne and ship based surveillance, satellite reconnaissance and so on. Homeland security requires effective means to negate these advanced overseeing systems. Researches show that CCD based EO systems can be significantly dazzled or even damaged by high-repetition rate pulsed lasers. Here, we report femto - second laser interaction with CCD camera, which is probable of great importance in future. Femto - second laser is quite fresh new lasers, which has unique characteristics, such as extremely short pulse width (1 fs = 10-15 s), extremely high peak power (1 TW = 1012W), and especially its unique features when interacting with matters. Researches in femto second laser interaction with materials (metals, dielectrics) clearly indicate non-thermal effect dominates the process, which is of vast difference from that of long pulses interaction with matters. Firstly, the damage threshold test are performed with femto second laser acting on the CCD camera. An 800nm, 500μJ, 100fs laser pulse is used to irradiate interline CCD solid-state image sensor in the experiment. In order to focus laser energy onto tiny CCD active cells, an optical system of F/5.6 is used. A Sony production CCDs are chose as typical targets. The damage threshold is evaluated with multiple test data. Point damage, line damage and full array damage were observed when the irradiated pulse energy continuously increase during the experiment. The point damage threshold is found 151.2 mJ/cm2.The line damage threshold is found 508.2 mJ/cm2.The full-array damage threshold is found to be 5.91 J/cm2. Although the phenomenon is almost the same as that of nano laser interaction with CCD, these damage thresholds are substantially lower than that of data obtained from nano second laser interaction with CCD. Then at the same time, the electric features after different degrees of damage are tested with electronic multi meter. The resistance values between clock signal lines are measured. Contrasting the resistance values of the CCD before and after damage, it is found that the resistances decrease significantly between the vertical transfer clock signal lines values. The same results are found between the vertical transfer clock signal line and the earth electrode (ground).At last, the damage position and the damage mechanism were analyzed with above results and SEM morphological experiments. The point damage results in the laser destroying material, which shows no macro electro influence. The line damage is quite different from that of point damage, which shows deeper material corroding effect. More importantly, short circuits are found between vertical clock lines. The full array damage is even more severe than that of line damage starring with SEM, while no obvious different electrical features than that of line damage are found. Further researches are anticipated in femto second laser caused CCD damage mechanism with more advanced tools. This research is valuable in EO countermeasure and/or laser shielding applications.

A PFM-based MWIR DROIC employing off-pixel fine conversion of photocharge to digital using integrated column ADCs

NASA Astrophysics Data System (ADS)

Abbasi, S.; Galioglu, A.; Shafique, A.; Ceylan, O.; Yazici, M.; Gurbuz, Y.

2017-02-01

A 32x32 prototype of a digital readout IC (DROIC) for medium-wave infrared focal plane arrays (MWIR IR-FPAs) is presented. The DROIC employs in-pixel photocurrent to digital conversion based on a pulse frequency modulation (PFM) loop and boasts a novel feature of off-pixel residue conversion using 10-bit column SAR ADCs. The remaining charge at the end of integration in typical PFM based digital pixel sensors is usually wasted. Previous works employing in-pixel extended counting methods make use of extra memory and counters to convert this left-over charge to digital, thereby performing fine conversion of the incident photocurrent. This results in a low quantization noise and hence keeps the readout noise low. However, focal plane arrays (FPAs) with small pixel pitch are constrained in pixel area, which makes it difficult to benefit from in-pixel extended counting circuitry. Thus, in this work, a novel approach to measure the residue outside the pixel using column -parallel SAR ADCs has been proposed. Moreover, a modified version of the conventional PFM based pixel has been designed to help hold the residue charge and buffer it to the column ADC. In addition to the 2D array of pixels, the prototype consists of 32 SAR ADCs, a timing controller block and a memory block to buffer the residue data coming out of the ADCs. The prototype has been designed and fabricated in 90nm CMOS.

The fingerprint nature of PCDD in iron ore sinter strand emissions, the effect of suppressants and alternative fuels, and the potential for comparison with the isomer profile of PCDF.

PubMed

Ooi, Tze C; Thompson, Dennis; Anderson, David R; Fisher, Ray

2018-01-01

It has been previously shown that the isomer profile of PCDF emissions from iron ore sinter plant only varies within limits even when suppressants or alternative fuels are added, to the extent that it can be said to have a 'fingerprint'. The isomer profiles of PCDD from tetra- to hexacholrodibenzo-p-dioxin from the same samples examined for PCDF emissions have been obtained, and show the same tendency for a 'fingerprint ' isomer distribution to occur. Occasional exceptionally high isomer abundances are observed, but these are uncommon. The potential for comparison of the abundances of PCDF and PCDD isomers with similar chlorination patterns to determine whether the same formation process is involved has been examined. It is found that co-elutions prevent extensive comparisons irrespective of whether the SP2331 or DB5ms column is used in the analyses for separation of isomers to provide the results used for comparisons, although they allow limited results to be obtained. It is suggested that analyses using the two chromatography columns to analyse the same sample in parallel could provide more resolution of the isomer profiles for use in comparisons. A pilot study using samples analysed using each column is limited because of detailed differences in the emissions profiles, but demonstrates that greater resolution is possible if the two columns are used to analyse one sample. Copyright © 2017 Elsevier Ltd. All rights reserved.

CCD Times of Minima of Selected Eclipsing Binaries

NASA Astrophysics Data System (ADS)

Zejda, Miloslav

2004-12-01

682 CCD minima observations of 259 eclipsing binaries made mainly by author are presented. The observed stars were chosen mainly from catalogue BRKA of observing programme of BRNO-Variable Star Section of CAS.

Modeling colony collapse disorder in honeybees as a contagion.

PubMed

Kribs-Zaleta, Christopher M; Mitchell, Christopher

2014-12-01

Honeybee pollination accounts annually for over $14 billion in United States agriculture alone. Within the past decade there has been a mysterious mass die-off of honeybees, an estimated 10 million beehives and sometimes as much as 90% of an apiary. There is still no consensus on what causes this phenomenon, called Colony Collapse Disorder, or CCD. Several mathematical models have studied CCD by only focusing on infection dynamics. We created a model to account for both healthy hive dynamics and hive extinction due to CCD, modeling CCD via a transmissible infection brought to the hive by foragers. The system of three ordinary differential equations accounts for multiple hive population behaviors including Allee effects and colony collapse. Numerical analysis leads to critical hive sizes for multiple scenarios and highlights the role of accelerated forager recruitment in emptying hives during colony collapse.

LSI arrays for space stations

NASA Technical Reports Server (NTRS)

Gassaway, J. D.

1976-01-01

Two approaches have been taken to study CCD's and some of their fundamental limitations. First a numerical analysis approach has been developed to solve the coupled transport and Poisson's equation for a thorough analysis of charge transfer in a CCD structure. The approach is formulated by treating the minority carriers as a surface distribution at the Si-SiO2 interface and setting up coupled difference equations for the charge and the potential. The SOR method is proposed for solving the two dimensional Poisson's equation for the potential. Methods are suggested for handling the discontinuities to improve convergence. Second, CCD shift registers were fabricated with parameters which should allow complete charge transfer independent of the transfer electrode gap width. A test instrument was designed and constructed which can be used to test this, or any similar, three phase CCD shift register.

Phase shifting white light interferometry using colour CCD for optical metrology and bio-imaging applications

NASA Astrophysics Data System (ADS)

Upputuri, Paul Kumar; Pramanik, Manojit

2018-02-01

Phase shifting white light interferometry (PSWLI) has been widely used for optical metrology applications because of their precision, reliability, and versatility. White light interferometry using monochrome CCD makes the measurement process slow for metrology applications. WLI integrated with Red-Green-Blue (RGB) CCD camera is finding imaging applications in the fields optical metrology and bio-imaging. Wavelength dependent refractive index profiles of biological samples were computed from colour white light interferograms. In recent years, whole-filed refractive index profiles of red blood cells (RBCs), onion skin, fish cornea, etc. were measured from RGB interferograms. In this paper, we discuss the bio-imaging applications of colour CCD based white light interferometry. The approach makes the measurement faster, easier, cost-effective, and even dynamic by using single fringe analysis methods, for industrial applications.

Noise analysis for CCD-based ultraviolet and visible spectrophotometry.

PubMed

Davenport, John J; Hodgkinson, Jane; Saffell, John R; Tatam, Ralph P

2015-09-20

We present the results of a detailed analysis of the noise behavior of two CCD spectrometers in common use, an AvaSpec-3648 CCD UV spectrometer and an Ocean Optics S2000 Vis spectrometer. Light sources used include a deuterium UV/Vis lamp and UV and visible LEDs. Common noise phenomena include source fluctuation noise, photoresponse nonuniformity, dark current noise, fixed pattern noise, and read noise. These were identified and characterized by varying light source, spectrometer settings, or temperature. A number of noise-limiting techniques are proposed, demonstrating a best-case spectroscopic noise equivalent absorbance of 3.5×10(-4)  AU for the AvaSpec-3648 and 5.6×10(-4)  AU for the Ocean Optics S2000 over a 30 s integration period. These techniques can be used on other CCD spectrometers to optimize performance.

Realization of Vilnius UPXYZVS photometric system for AltaU42 CCD camera at the MAO NAS of Ukraine

NASA Astrophysics Data System (ADS)

Vid'Machenko, A. P.; Andruk, V. M.; Samoylov, V. S.; Delets, O. S.; Nevodovsky, P. V.; Ivashchenko, Yu. M.; Kovalchuk, G. U.

2005-06-01

The description of two-inch glass filters of the Vilnius UPXYZVS photometric system, which are made at the Main Astronomical Observatory of NAS of Ukraine for AltaU42 CCD camera with format of 2048×2048 pixels, is presented in the paper. Reaction curves of instrumental system are shown. Estimations of minimal star's magnitudes for each filter's band in comparison with the visual V one are obtained. New software for automation of CCD frames processing is developed in program shell of LINUX/MIDAS/ROMAFOT. It is planned to carry out observations with the purpose to create the catalogue of primary UPXYZVS CCD standards in selected field of the sky for some radio-sources, globular and open clusters, etc. Numerical estimations of astrometric and photometric accuracy are obtained.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aoyagi, Yasuyuki; Department of Genome Research and Clinical Application, Graduate School of Medicine, Chiba University, Chiba; Kuroda, Masayuki, E-mail: kurodam@faculty.chiba-u.jp

Adipose tissue is expected to provide a source of cells for protein replacement therapies via auto-transplantation. However, the conditioning of the environment surrounding the transplanted adipocytes for their long-term survival and protein secretion properties has not been established. We have recently developed a preparation procedure for preadipocytes, ceiling culture-derived proliferative adipocytes (ccdPAs), as a therapeutic gene vehicle suitable for stable gene product secretion. We herein report the results of our evaluation of using fibrin glue as a scaffold for the transplanted ccdPAs for the expression of a transduced gene in a three-dimensional culture system. The ccdPAs secreted the functional proteinmore » translated from an exogenously transduced gene, as well as physiological adipocyte proteins, and the long viability of ccdPAs (up to 84 days) was dependent on the fibrinogen concentrations. The ccdPAs spontaneously accumulated lipid droplets, and their expression levels of the transduced exogenous gene with its product were maintained for at least 56 days. The fibrinogen concentration modified the adipogenic differentiation of ccdPAs and their exogenous gene expression levels, and the levels of exogenously transduced gene expression at the different fibrinogen concentrations were dependent on the extent of adipogenic differentiation in the gel. These results indicate that fibrin glue helps to maintain the high adipogenic potential of cultured adipocytes after passaging in a 3D culture system, and suggests that once they are successfully implanted at the transplantation site, the cells exhibit increased expression of the transduced gene with adipogenic differentiation.« less

The Cross-Cultural Dementia Screening (CCD): A new neuropsychological screening instrument for dementia in elderly immigrants.

PubMed

Goudsmit, Miriam; Uysal-Bozkir, Özgül; Parlevliet, Juliette L; van Campen, Jos P C M; de Rooij, Sophia E; Schmand, Ben

2017-03-01

Currently, approximately 3.9% of the European population are non-EU citizens, and a large part of these people are from "non-Western" societies, such as Turkey and Morocco. For various reasons, the incidence of dementia in this group is expected to increase. However, cognitive testing is challenging due to language barriers and low education and/or illiteracy. The newly developed Cross-Cultural Dementia Screening (CCD) can be administered without an interpreter. It contains three subtests that assess memory, mental speed, and executive function. We hypothesized the CCD to be a culture-fair test that could discriminate between demented patients and cognitively healthy controls. To test this hypothesis, 54 patients who had probable dementia were recruited via memory clinics. Controls (N = 1625) were recruited via their general practitioners. All patients and controls were aged 55 years and older and of six different self-defined ethnicities (Dutch, Turkish, Moroccan-Arabic, Moroccan-Berber, Surinamese-Creole, and Surinamese-Hindustani). Exclusion criteria included current or previous conditions that affect cognitive functioning. There were performance differences between the ethnic groups, but these disappeared after correcting for age and education differences between the groups, which supports our central hypothesis that the CCD is a culture-fair test. Receiver-operating characteristic (ROC) and logistic regression analyses showed that the CCD has high predictive validity for dementia (sensitivity: 85%; specificity: 89%). The CCD is a sensitive and culture-fair neuropsychological instrument for dementia screening in low-educated immigrant populations.

Nano-JASMINE: cosmic radiation degradation of CCD performance and centroid detection

NASA Astrophysics Data System (ADS)

Kobayashi, Yukiyasu; Shimura, Yuki; Niwa, Yoshito; Yano, Taihei; Gouda, Naoteru; Yamada, Yoshiyuki

2012-09-01

Nano-JASMINE (NJ) is a very small astrometry satellite project led by the National Astronomical Observatory of Japan. The satellite is ready for launch, and the launch is currently scheduled for late 2013 or early 2014. The satellite is equipped with a fully depleted CCD and is expected to perform astrometry observations for stars brighter than 9 mag in the zw-band (0.6 µm-1.0 µm). Distances of stars located within 100 pc of the Sun can be determined by using annual parallax measurements. The targeted accuracy for the position determination of stars brighter than 7.5 mag is 3 mas, which is equivalent to measuring the positions of stars with an accuracy of less than one five-hundredth of the CCD pixel size. The position measurements of stars are performed by centroiding the stellar images taken by the CCD that operates in the time and delay integration mode. The degradation of charge transfer performance due to cosmic radiation damage in orbit is proved experimentally. A method is then required to compensate for the effects of performance degradation. One of the most effective ways of achieving this is to simulate observed stellar outputs, including the effect of CCD degradation, and then formulate our centroiding algorithm and evaluate the accuracies of the measurements. We report here the planned procedure to simulate the outputs of the NJ observations. We also developed a CCD performance-measuring system and present preliminary results obtained using the system.

The chemical component dictionary: complete descriptions of constituent molecules in experimentally determined 3D macromolecules in the Protein Data Bank.

PubMed

Westbrook, John D; Shao, Chenghua; Feng, Zukang; Zhuravleva, Marina; Velankar, Sameer; Young, Jasmine

2015-04-15

The Chemical Component Dictionary (CCD) is a chemical reference data resource that describes all residue and small molecule components found in Protein Data Bank (PDB) entries. The CCD contains detailed chemical descriptions for standard and modified amino acids/nucleotides, small molecule ligands and solvent molecules. Each chemical definition includes descriptions of chemical properties such as stereochemical assignments, chemical descriptors, systematic chemical names and idealized coordinates. The content, preparation, validation and distribution of this CCD chemical reference dataset are described. The CCD is updated regularly in conjunction with the scheduled weekly release of new PDB structure data. The CCD and amino acid variant reference datasets are hosted in the public PDB ftp repository at ftp://ftp.wwpdb.org/pub/pdb/data/monomers/components.cif.gz, ftp://ftp.wwpdb.org/pub/pdb/data/monomers/aa-variants-v1.cif.gz, and its mirror sites, and can be accessed from http://wwpdb.org. jwest@rcsb.rutgers.edu. Supplementary data are available at Bioinformatics online. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

GPS-CCD: A Novel Computational Program for the Prediction of Calpain Cleavage Sites

PubMed Central

Gao, Xinjiao; Ma, Qian; Ren, Jian; Xue, Yu

2011-01-01

As one of the most essential post-translational modifications (PTMs) of proteins, proteolysis, especially calpain-mediated cleavage, plays an important role in many biological processes, including cell death/apoptosis, cytoskeletal remodeling, and the cell cycle. Experimental identification of calpain targets with bona fide cleavage sites is fundamental for dissecting the molecular mechanisms and biological roles of calpain cleavage. In contrast to time-consuming and labor-intensive experimental approaches, computational prediction of calpain cleavage sites might more cheaply and readily provide useful information for further experimental investigation. In this work, we constructed a novel software package of GPS-CCD (Calpain Cleavage Detector) for the prediction of calpain cleavage sites, with an accuracy of 89.98%, sensitivity of 60.87% and specificity of 90.07%. With this software, we annotated potential calpain cleavage sites for hundreds of calpain substrates, for which the exact cleavage sites had not been previously determined. In this regard, GPS-CCD 1.0 is considered to be a useful tool for experimentalists. The online service and local packages of GPS-CCD 1.0 were implemented in JAVA and are freely available at: http://ccd.biocuckoo.org/. PMID:21533053

3D morphology reconstruction using linear array CCD binocular stereo vision imaging system

NASA Astrophysics Data System (ADS)

Pan, Yu; Wang, Jinjiang

2018-01-01

Binocular vision imaging system, which has a small field of view, cannot reconstruct the 3-D shape of the dynamic object. We found a linear array CCD binocular vision imaging system, which uses different calibration and reconstruct methods. On the basis of the binocular vision imaging system, the linear array CCD binocular vision imaging systems which has a wider field of view can reconstruct the 3-D morphology of objects in continuous motion, and the results are accurate. This research mainly introduces the composition and principle of linear array CCD binocular vision imaging system, including the calibration, capture, matching and reconstruction of the imaging system. The system consists of two linear array cameras which were placed in special arrangements and a horizontal moving platform that can pick up objects. The internal and external parameters of the camera are obtained by calibrating in advance. And then using the camera to capture images of moving objects, the results are then matched and 3-D reconstructed. The linear array CCD binocular vision imaging systems can accurately measure the 3-D appearance of moving objects, this essay is of great significance to measure the 3-D morphology of moving objects.

Designing payment for Collaborative Care for Depression in primary care.

PubMed

Bao, Yuhua; Casalino, Lawrence P; Ettner, Susan L; Bruce, Martha L; Solberg, Leif I; Unützer, Jürgen

2011-10-01

To design a bundled case rate for Collaborative Care for Depression (CCD) that aligns incentives with evidence-based depression care in primary care. A clinical information system used by all care managers in a randomized controlled trial of CCD for older primary care patients. We conducted an empirical investigation of factors accounting for variation in CCD resource use over time and across patients. CCD resource use at the patient-episode and patient-month levels was measured by number of care manager contacts and direct patient contact time and analyzed with count data (Poisson or negative binomial) models. Episode-level resource use varies substantially with patient's time in the program. Monthly use declines sharply in the first 6 months regardless of treatment response or remission status, but it remains stable afterwards. An adjusted episode or monthly case rate design better matches payment with variation in resource use compared with a fixed design. Our findings lend support to an episode payment adjusted by number of months receiving CCD and a monthly payment adjusted by the ordinal month. Nonpayment tools including program certification and performance evaluation and reward systems are needed to fully align incentives. © Health Research and Educational Trust.

Research of optical coherence tomography microscope based on CCD detector

NASA Astrophysics Data System (ADS)

Zhang, Hua; Xu, Zhongbao; Zhang, Shuomo

2008-12-01

The reference wave phase was modulated with a sinusoidal vibrating mirror attached to a Piezoelectric Transducer (PZT), the integration was performed by a CCD, and the charge storage period of the CCD image sensor was one-quarter period of the sinusoidal phase modulation. With the frequency- synchronous detection technique, four images (four frames of interference pattern) were recorded during one period of the phase modulation. In order to obtain the optimum modulation parameter, the values of amplitude and phase of the sinusoidal phase modulation were determined by considering the measurement error caused by the additive noise contained in the detected values. The PZT oscillation was controlled by a closed loop control system based on PID controller. An ideal discrete digital sine function at 50Hz with adjustable amplitude was used to adjust the vibrating of PZT, and a digital phase shift techniques was used to adjust vibrating phase of PZT so that the phase of the modulation could reach their optimum values. The CCD detector was triggered with software at 200Hz. Based on work above a small coherent signal masked by the preponderant incoherent background with a CCD detector was obtained.

eJAAVSO | aavso.org

Science.gov Websites

Institute CCD School Videos Student Projects Two Eyes, 3D Variable Star Astronomy H-R Diagram Plotting CHOICE Online Institute CCD School Videos Student Projects Two Eyes, 3D Variable Star Astronomy H-R

Back-illuminated large area frame transfer CCDs for space-based hyper-spectral imaging applications

NASA Astrophysics Data System (ADS)

Philbrick, Robert H.; Gilmore, Angelo S.; Schrein, Ronald J.

2016-07-01

Standard offerings of large area, back-illuminated full frame CCD sensors are available from multiple suppliers and they continue to be commonly deployed in ground- and space-based applications. By comparison the availability of large area frame transfers CCDs is sparse, with the accompanying 2x increase in die area no doubt being a contributing factor. Modern back-illuminated CCDs yield very high quantum efficiency in the 290 to 400 nm band, a wavelength region of great interest in space-based instruments studying atmospheric phenomenon. In fast framing (e.g. 10 - 20 Hz), space-based applications such as hyper-spectral imaging, the use of a mechanical shutter to block incident photons during readout can prove costly and lower instrument reliability. The emergence of large area, all-digital visible CMOS sensors, with integrate while read functionality, are an alternative solution to CCDs; but, even after factoring in reduced complexity and cost of support electronics, the present cost to implement such novel sensors is prohibitive to cost constrained missions. Hence, there continues to be a niche set of applications where large area, back-illuminated frame transfer CCDs with high UV quantum efficiency, high frame rate, high full well, and low noise provide an advantageous solution. To address this need a family of large area frame transfer CCDs has been developed that includes 2048 (columns) x 256 (rows) (FT4), 2048 x 512 (FT5), and 2048 x 1024 (FT6) full frame transfer CCDs; and a 2048 x 1024 (FT7) split-frame transfer CCD. Each wafer contains 4 FT4, 2 FT5, 2 FT6, and 2 FT7 die. The designs have undergone radiation and accelerated life qualification and the electro-optical performance of these CCDs over the wavelength range of 290 to 900 nm is discussed.

Reduction of product-related species during the fermentation and purification of a recombinant IL-1 receptor antagonist at the laboratory and pilot scale.

PubMed

Schirmer, Emily B; Golden, Kathryn; Xu, Jin; Milling, Jesse; Murillo, Alec; Lowden, Patricia; Mulagapati, Srihariraju; Hou, Jinzhao; Kovalchin, Joseph T; Masci, Allyson; Collins, Kathryn; Zarbis-Papastoitsis, Gregory

2013-08-01

Through a parallel approach of tracking product quality through fermentation and purification development, a robust process was designed to reduce the levels of product-related species. Three biochemically similar product-related species were identified as byproducts of host-cell enzymatic activity. To modulate intracellular proteolytic activity, key fermentation parameters (temperature, pH, trace metals, EDTA levels, and carbon source) were evaluated through bioreactor optimization, while balancing negative effects on growth, productivity, and oxygen demand. The purification process was based on three non-affinity steps and resolved product-related species by exploiting small charge differences. Using statistical design of experiments for elution conditions, a high-resolution cation exchange capture column was optimized for resolution and recovery. Further reduction of product-related species was achieved by evaluating a matrix of conditions for a ceramic hydroxyapatite column. The optimized fermentation process was transferred from the 2-L laboratory scale to the 100-L pilot scale and the purification process was scaled accordingly to process the fermentation harvest. The laboratory- and pilot-scale processes resulted in similar process recoveries of 60 and 65%, respectively, and in a product that was of equal quality and purity to that of small-scale development preparations. The parallel approach for up- and downstream development was paramount in achieving a robust and scalable clinical process. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Evaluation of large format electron bombarded virtual phase CCDs as ultraviolet imaging detectors

NASA Technical Reports Server (NTRS)

Opal, Chet B.; Carruthers, George R.

1989-01-01

In conjunction with an external UV-sensitive cathode, an electron-bombarded CCD may be used as a high quantum efficiency/wide dynamic range photon-counting UV detector. Results are presented for the case of a 1024 x 1024, 18-micron square pixel virtual phase CCD used with an electromagnetically focused f/2 Schmidt camera, which yields excellent simgle-photoevent discrimination and counting efficiency. Attention is given to the vacuum-chamber arrangement used to conduct system tests and the CCD electronics and data-acquisition systems employed.

Optical synthesizer for a large quadrant-array CCD camera: Center director's discretionary fund

NASA Technical Reports Server (NTRS)

Hagyard, Mona J.

1992-01-01

The objective of this program was to design and develop an optical device, an optical synthesizer, that focuses four contiguous quadrants of a solar image on four spatially separated CCD arrays that are part of a unique CCD camera system. This camera and the optical synthesizer will be part of the new NASA-Marshall Experimental Vector Magnetograph, and instrument developed to measure the Sun's magnetic field as accurately as present technology allows. The tasks undertaken in the program are outlined and the final detailed optical design is presented.

InSb arrays with CCD readout for 1.0- to 5.5-microns infrared applications

NASA Technical Reports Server (NTRS)

Phillips, J. D.; Scorso, J. B.; Thom, R. D.

1976-01-01

There were two approaches for fabricating indium antimonide (InSb) arrays with CCD readout discussed. The hybrid approach integrated InSb detectors and silicon CCDs in a modular assembly via an advanced interconnection technology. In the monolithic approach, the InSb infrared detectors and the CCD readout were integrated on the same InSb chip. Both approaches utilized intrinsic (band-to-band) photodetection with the attendant advantages over extrinsic detectors. The status of each of these detector readout concepts, with pertinent performance characteristics, was presented.

A design of driving circuit for star sensor imaging camera

NASA Astrophysics Data System (ADS)

Li, Da-wei; Yang, Xiao-xu; Han, Jun-feng; Liu, Zhao-hui

2016-01-01

The star sensor is a high-precision attitude sensitive measuring instruments, which determine spacecraft attitude by detecting different positions on the celestial sphere. Imaging camera is an important portion of star sensor. The purpose of this study is to design a driving circuit based on Kodak CCD sensor. The design of driving circuit based on Kodak KAI-04022 is discussed, and the timing of this CCD sensor is analyzed. By the driving circuit testing laboratory and imaging experiments, it is found that the driving circuits can meet the requirements of Kodak CCD sensor.

Construction of a photochemical reactor combining a CCD spectrophotometer and a LED radiation source.

PubMed

Gombár, Melinda; Józsa, Éva; Braun, Mihály; Ősz, Katalin

2012-10-01

An inexpensive photoreactor using LED light sources and a fibre-optic CCD spectrophotometer as a detector was built by designing a special cell holder for standard 1.000 cm cuvettes. The use of this device was demonstrated by studying the aqueous photochemical reaction of 2,5-dichloro-1,4-benzoquinone. The developed method combines the highly quantitative data collection of CCD spectrophotometers with the possibility of illuminating the sample independently of the detecting light beam, which is a substantial improvement of the method using diode array spectrophotometers as photoreactors.

NEUTRON RADIATION DAMAGE IN CCD CAMERAS AT JOINT EUROPEAN TORUS (JET).

PubMed

Milocco, Alberto; Conroy, Sean; Popovichev, Sergey; Sergienko, Gennady; Huber, Alexander

2017-10-26

The neutron and gamma radiations in large fusion reactors are responsible for damage to charged couple device (CCD) cameras deployed for applied diagnostics. Based on the ASTM guide E722-09, the 'equivalent 1 MeV neutron fluence in silicon' was calculated for a set of CCD cameras at the Joint European Torus. Such evaluations would be useful to good practice in the operation of the video systems. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

A finite parallel zone model to interpret and extend Giddings' coupling theory for the eddy-dispersion in porous chromatographic media.

PubMed

Desmet, Gert

2013-11-01

The finite length parallel zone (FPZ)-model is proposed as an alternative model for the axial- or eddy-dispersion caused by the occurrence of local velocity biases or flow heterogeneities in porous media such as those used in liquid chromatography columns. The mathematical plate height expression evolving from the model shows that the A- and C-term band broadening effects that can originate from a given velocity bias should be coupled in an exponentially decaying way instead of harmonically as proposed in Giddings' coupling theory. In the low and high velocity limit both models converge, while a 12% difference can be observed in the (practically most relevant) intermediate range of reduced velocities. Explicit expressions for the A- and C-constants appearing in the exponential decay-based plate height expression have been derived for each of the different possible velocity bias levels (single through-pore and particle level, multi-particle level and trans-column level). These expressions allow to directly relate the band broadening originating from these different levels to the local fundamental transport parameters, hence offering the possibility to include a velocity-dependent and, if, needed retention factor-dependent transversal dispersion coefficient. Having developed the mathematics for the general case wherein a difference in retention equilibrium establishes between the two parallel zones, the effect of any possible local variations in packing density and/or retention capacity on the eddy-dispersion can be explicitly accounted for as well. It is furthermore also shown that, whereas the lumped transport parameter model used in the basic variant of the FPZ-model only provides a first approximation of the true decay constant, the model can be extended by introducing a constant correction factor to correctly account for the continuous transversal dispersion transport in the velocity bias zones. Copyright © 2013 Elsevier B.V. All rights reserved.

A method of immediate detection of objects with a near-zero apparent motion in series of CCD-frames

NASA Astrophysics Data System (ADS)

Savanevych, V. E.; Khlamov, S. V.; Vavilova, I. B.; Briukhovetskyi, A. B.; Pohorelov, A. V.; Mkrtichian, D. E.; Kudak, V. I.; Pakuliak, L. K.; Dikov, E. N.; Melnik, R. G.; Vlasenko, V. P.; Reichart, D. E.

2018-01-01

The paper deals with a computational method for detection of the solar system minor bodies (SSOs), whose inter-frame shifts in series of CCD-frames during the observation are commensurate with the errors in measuring their positions. These objects have velocities of apparent motion between CCD-frames not exceeding three rms errors (3σ) of measurements of their positions. About 15% of objects have a near-zero apparent motion in CCD-frames, including the objects beyond the Jupiter's orbit as well as the asteroids heading straight to the Earth. The proposed method for detection of the object's near-zero apparent motion in series of CCD-frames is based on the Fisher f-criterion instead of using the traditional decision rules that are based on the maximum likelihood criterion. We analyzed the quality indicators of detection of the object's near-zero apparent motion applying statistical and in situ modeling techniques in terms of the conditional probability of the true detection of objects with a near-zero apparent motion. The efficiency of method being implemented as a plugin for the Collection Light Technology (CoLiTec) software for automated asteroids and comets detection has been demonstrated. Among the objects discovered with this plugin, there was the sungrazing comet C/2012 S1 (ISON). Within 26 min of the observation, the comet's image has been moved by three pixels in a series of four CCD-frames (the velocity of its apparent motion at the moment of discovery was equal to 0.8 pixels per CCD-frame; the image size on the frame was about five pixels). Next verification in observations of asteroids with a near-zero apparent motion conducted with small telescopes has confirmed an efficiency of the method even in bad conditions (strong backlight from the full Moon). So, we recommend applying the proposed method for series of observations with four or more frames.

The Gene Expression Profile of CD11c+CD8α− Dendritic Cells in the Pre-Diabetic Pancreas of the NOD Mouse

PubMed Central

Beumer, Wouter; Welzen-Coppens, Jojanneke M. C.; van Helden-Meeuwsen, Cornelia G.; Gibney, Sinead M.; Drexhage, Hemmo A.; Versnel, Marjan A.

2014-01-01

Two major dendritic cell (DC) subsets have been described in the pancreas of mice: The CD11c+CD8α− DCs (strong CD4+ T cell proliferation inducers) and the CD8α+CD103+ DCs (T cell apoptosis inducers). Here we analyzed the larger subset of CD11c+CD8α− DCs isolated from the pancreas of pre-diabetic NOD mice for genome-wide gene expression (validated by Q-PCR) to elucidate abnormalities in underlying gene expression networks. CD11c+CD8α− DCs were isolated from 5 week old NOD and control C57BL/6 pancreas. The steady state pancreatic NOD CD11c+CD8α− DCs showed a reduced expression of several gene networks important for the prime functions of these cells, i.e. for cell renewal, immune tolerance induction, migration and for the provision of growth factors including those for beta cell regeneration. A functional in vivo BrdU incorporation test showed the reduced proliferation of steady state pancreatic DC. The reduced expression of tolerance induction genes (CD200R, CCR5 and CD24) was supported on the protein level by flow cytometry. Also previously published functional tests on maturation, immune stimulation and migration confirm the molecular deficits of NOD steady state DC. Despite these deficiencies NOD pancreas CD11c+CD8α− DCs showed a hyperreactivity to LPS, which resulted in an enhanced pro-inflammatory state characterized by a gene profile of an enhanced expression of a number of classical inflammatory cytokines. The enhanced up-regulation of inflammatory genes was supported by the in vitro cytokine production profile of the DCs. In conclusion, our data show that NOD pancreatic CD11c+CD8α− DCs show various deficiencies in steady state, while hyperreactive when encountering a danger signal such as LPS. PMID:25166904

Application of the CCD Fabry-Perot Annular Summing Technique to Thermospheric O(1)D.

NASA Astrophysics Data System (ADS)

Coakley, Monica Marie

1995-01-01

This work will detail the verification of the advantages of the Fabry-Perot charge coupled device (CCD) annular summing technique, the development of the technique for analysis of daysky spectra, and the implications of the resulting spectra for neutral temperature and wind measurements in the daysky thermosphere. The daysky spectral feature of interest is the bright (1 kilo-Rayleigh) thermospheric (OI) emission at 6300 A which had been observed in the nightsky in order to determine winds and temperatures in the vicinity of the altitude of 250 km. In the daysky, the emission line sits on top of a bright Rayleigh scattered continuum background which significantly complicates the observation. With a triple etalon Fabry-Perot spectrometer, the continuum background can be reduced while maintaining high throughput and high resolution. The inclusion of a CCD camera results in significant savings in integration time over the two more standard scanning photomultiplier systems that have made the same wind and temperature measurements in the past. A comparable CCD system can experience an order of magnitude savings in integration time over a PMT system. Laboratory and field tests which address the advantages and limitations of both the Fabry-Perot CCD annular summing technique and the daysky CCD imaging are included in Chap. 2 and Chap. 3. With a sufficiently large throughput associated with the spectrometer and a CCD detector, rapid observations (~4 minute integrations) can be made. Extraction of the line width and line center from the daysky near-continuum background is complicated compared to the nightsky case, but possible. Methods of fitting the line are included in Chap. 4. The daysky O ^1D temperatures are consistent with a lower average emission height than predicted by models. The data and models are discussed in Chap. 5. Although some discrepancies exist between resulting temperatures and models, the observations indicate the potential for other direct measurements of bright neutral species in the daysky as well as the potential for twenty-four hour coverage.

Flat Field Anomalies in an X-ray CCD Camera Measured Using a Manson X-ray Source

DOE Office of Scientific and Technical Information (OSTI.GOV)

M. J. Haugh and M. B. Schneider

2008-10-31

The Static X-ray Imager (SXI) is a diagnostic used at the National Ignition Facility (NIF) to measure the position of the X-rays produced by lasers hitting a gold foil target. The intensity distribution taken by the SXI camera during a NIF shot is used to determine how accurately NIF can aim laser beams. This is critical to proper NIF operation. Imagers are located at the top and the bottom of the NIF target chamber. The CCD chip is an X-ray sensitive silicon sensor, with a large format array (2k x 2k), 24 μm square pixels, and 15 μm thick. Amore » multi-anode Manson X-ray source, operating up to 10kV and 10W, was used to characterize and calibrate the imagers. The output beam is heavily filtered to narrow the spectral beam width, giving a typical resolution E/ΔE≈10. The X-ray beam intensity was measured using an absolute photodiode that has accuracy better than 1% up to the Si K edge and better than 5% at higher energies. The X-ray beam provides full CCD illumination and is flat, within ±1% maximum to minimum. The spectral efficiency was measured at 10 energy bands ranging from 930 eV to 8470 eV. We observed an energy dependent pixel sensitivity variation that showed continuous change over a large portion of the CCD. The maximum sensitivity variation occurred at 8470 eV. The geometric pattern did not change at lower energies, but the maximum contrast decreased and was not observable below 4 keV. We were also able to observe debris, damage, and surface defects on the CCD chip. The Manson source is a powerful tool for characterizing the imaging errors of an X-ray CCD imager. These errors are quite different from those found in a visible CCD imager.« less

SO2 camera measurements at Lastarria volcano and Lascar volcano in Chile

NASA Astrophysics Data System (ADS)

Lübcke, Peter; Bobrowski, Nicole; Dinger, Florian; Klein, Angelika; Kuhn, Jonas; Platt, Ulrich

2015-04-01

The SO2 camera is a remote-sensing technique that measures volcanic SO2 emissions via the strong SO2 absorption structures in the UV using scattered solar radiation as a light source. The 2D-imagery (usually recorded with a frame rate of up to 1 Hz) allows new insights into degassing processes of volcanoes. Besides the large advantage of high frequency sampling the spatial resolution allows to investigate SO2 emissions from individual fumaroles and not only the total SO2 emission flux of a volcano, which is often dominated by the volcanic plume. Here we present SO2 camera measurements that were made during the CCVG workshop in Chile in November 2014. Measurements were performed at Lastarria volcano, a 5700 m high stratovolcano and Lascar volcano, a 5600 m high stratovolcano both in northern Chile on 21 - 22 November, 2014 and on 26 - 27 November, 2014, respectively. At both volcanoes measurements were conducted from a distance of roughly 6-7 km under close to ideal conditions (low solar zenith angle, a very dry and cloudless atmosphere and an only slightly condensed plume). However, determination of absolute SO2 emission rates proves challenging as part of the volcanic plume hovered close to the ground. The volcanic plume therefore is in front of the mountain in our camera images. An SO2 camera system consisting of a UV sensitive CCD and two UV band-pass filters (centered at 315 nm and 330 nm) was used. The two band-pass filters are installed in a rotating wheel and images are taken with both filter sequentially. The instrument used a CCD with 1024 x 1024 pixels and an imaging area of 13.3 mm x 13.3 mm. In combination with the focal length of 32 mm this results in a field-of-view of 25° x 25°. The calibration of the instrument was performed with help of a DOAS instrument that is co-aligned with the SO2 camera. We will present images and SO2 emission rates from both volcanoes. At Lastarria gases are emitted from three different fumarole fields and we will attempt to investigate the degassing behavior of the individual fumaroles. Lascar volcano only had a very weak plume originating from the active central crater with maximum SO2 column densities of only up to 5 × 1017[molecules/cm2] during our measurements. These low SO2 column densities in combination with the almost ideal measurements conditions will be used to assess the detection limit of our current SO2 camera system.

CCD Times of Minima of Faint Eclipsing Binaries in 2000

NASA Astrophysics Data System (ADS)

Zejda, Miloslav

2002-06-01

196 CCD minima observations of 122 eclipsing binaries made by the author in 2000 are presented. The observed stars were chosen from the catalogue BRKA of observing programme of BRNO-Variable Star Section of CAS.

Active Pixel Sensors: Are CCD's Dinosaurs?

NASA Technical Reports Server (NTRS)

Fossum, Eric R.

1993-01-01

Charge-coupled devices (CCD's) are presently the technology of choice for most imaging applications. In the 23 years since their invention in 1970, they have evolved to a sophisticated level of performance. However, as with all technologies, we can be certain that they will be supplanted someday. In this paper, the Active Pixel Sensor (APS) technology is explored as a possible successor to the CCD. An active pixel is defined as a detector array technology that has at least one active transistor within the pixel unit cell. The APS eliminates the need for nearly perfect charge transfer -- the Achilles' heel of CCDs. This perfect charge transfer makes CCD's radiation 'soft,' difficult to use under low light conditions, difficult to manufacture in large array sizes, difficult to integrate with on-chip electronics, difficult to use at low temperatures, difficult to use at high frame rates, and difficult to manufacture in non-silicon materials that extend wavelength response.

Degradation of optical components in space

NASA Technical Reports Server (NTRS)

Blue, M. D.

1993-01-01

This report concerns two types of optical components: multilayer filters and mirrors, and self-scanned imaging arrays using charge coupled device (CCD) readouts. For the filters and mirrors, contamination produces a strong reduction in transmittance in the ultraviolet spectral region, but has little or no effect in the visible and infrared spectral regions. Soft substrates containing halides are unsatisfactory as windows or substrates. Materials choice for dielectric layers should also reflect such considerations. Best performance is also found for the harder materials. Compaction of the layers and interlayer diffusion causes a blue shift in center wavelength and loss of throughput. For sensors using CCD's, shifts in gate voltage and reductions in transfer efficiency occur. Such effects in CCD's are in accord with expectations of the effects of the radiation dose on the device. Except for optical fiber, degradation of CCD's represents the only ionizing-radiation induced effect on the Long Duration Exposure Facility (LDEF) optical systems components that has been observed.

Measurement of phase function of aerosol at different altitudes by CCD Lidar

NASA Astrophysics Data System (ADS)

Sun, Peiyu; Yuan, Ke'e.; Yang, Jie; Hu, Shunxing

2018-02-01

The aerosols near the ground are closely related to human health and climate change, the study on which has important significance. As we all know, the aerosol is inhomogeneous at different altitudes, of which the phase function is also different. In order to simplify the retrieval algorithm, it is usually assumed that the aerosol is uniform at different altitudes, which will bring measurement error. In this work, an experimental approach is demonstrated to measure the scattering phase function of atmospheric aerosol particles at different heights by CCD lidar system, which could solve the problem of the traditional CCD lidar system in assumption of phase function. The phase functions obtained by the new experimental approach are used to retrieve the aerosol extinction coefficient profiles. By comparison of the aerosol extinction coefficient retrieved by Mie-scattering aerosol lidar and CCD lidar at night, the reliability of new experimental approach is verified.

Direct detection of x-rays for protein crystallography employing a thick, large area CCD

DOEpatents

Atac, Muzaffer; McKay, Timothy

1999-01-01

An apparatus and method for directly determining the crystalline structure of a protein crystal. The crystal is irradiated by a finely collimated x-ray beam. The interaction of the x-ray beam with the crystal produces scattered x-rays. These scattered x-rays are detected by means of a large area, thick CCD which is capable of measuring a significant number of scattered x-rays which impact its surface. The CCD is capable of detecting the position of impact of the scattered x-ray on the surface of the CCD and the quantity of scattered x-rays which impact the same cell or pixel. This data is then processed in real-time and the processed data is outputted to produce a image of the structure of the crystal. If this crystal is a protein the molecular structure of the protein can be determined from the data received.

Constraining neutrinos as background to wimp-nucleon dark matter particle searches for DaMIC: CCD physics analysis and electronics development

NASA Astrophysics Data System (ADS)

Butner, Melissa Jean

The DaMIC (Dark Matter in CCDs) experiment searches for dark matter particles using charge coupled devices (CCDs) operated at a low detection threshold of ˜40 eV electron equivalent energy (eVee). A multiplexor board is tested for DAMIC100+ which has the ability to control up to 16 CCDs at one time allowing for the selection of a single CCD for readout while leaving all others static and maintaining sub-electron noise. A dark matter limit is produced using the results of physics data taken with the DAMIC experiment. Next, the contribution from neutrino-nucleus coherent scattering is investigated using data from the Coherent Neutrino Nucleus Interaction Experiment (CONnuIE) using the same CCD technology. The results are used to explore the performance of CCD detectors that ultimately will limit the ability to differentiate incident solar and atmospheric neutrinos from dark matter particles.

Colorimetric monitoring of nanometer distance changes in DNA-templated plasmon rulers (Conference Presentation)

NASA Astrophysics Data System (ADS)

Lermusiaux, Laurent; Bidault, Sebastien

2016-03-01

The nanometer-scale sensitivity of plasmon coupling allows the translation of minute morphological changes in nanostructures into macroscopic optical signals. In particular, single nanostructure scattering spectroscopy provides a direct estimation of interparticle distances in gold nanoparticle (AuNP) dimers linked by a short DNA double-strand [M. P. Busson et al, Nano Lett. 11, 5060 (2011)]. We demonstrate here that this spectroscopic information can be inferred from simple widefield measurements on a calibrated color camera [L. Lermusiaux et al, ACS Nano 9, 978 (2015)]. This allows us to analyze the influence of electrostatic and steric interparticle interactions on the morphology of DNA-templated AuNP groupings. Furthermore, polarization-resolved measurements on a color CCD provide a parallel imaging of AuNP dimer orientations. We apply this spectroscopic characterization to identify dimers featuring two different conformations of the same DNA template. In practice, the biomolecular scaffold contains a hairpin-loop that opens after hybridization to a specific DNA sequence and increases the interparticle distance [L. Lermusiaux et al, ACS Nano 6, 10992 (2012)]. These results open exciting perspectives for the parallel sensing of single specific DNA strands using plasmon rulers. We discuss the limits of this approach in terms of the physicochemical stability and reactivity of these nanostructures and demonstrate the importance of engineering the AuNP surface chemistry, in particular using amphiphilic ligands [L. Lermusiaux and S. Bidault, Small (2015), in press].

PN-CCD camera for XMM: performance of high time resolution/bright source operating modes

NASA Astrophysics Data System (ADS)

Kendziorra, Eckhard; Bihler, Edgar; Grubmiller, Willy; Kretschmar, Baerbel; Kuster, Markus; Pflueger, Bernhard; Staubert, Ruediger; Braeuninger, Heinrich W.; Briel, Ulrich G.; Meidinger, Norbert; Pfeffermann, Elmar; Reppin, Claus; Stoetter, Diana; Strueder, Lothar; Holl, Peter; Kemmer, Josef; Soltau, Heike; von Zanthier, Christoph

1997-10-01

The pn-CCD camera is developed as one of the focal plane instruments for the European photon imaging camera (EPIC) on board the x-ray multi mirror (XMM) mission to be launched in 1999. The detector consists of four quadrants of three pn-CCDs each, which are integrated on one silicon wafer. Each CCD has 200 by 64 pixels (150 micrometer by 150 micrometers) with 280 micrometers depletion depth. One CCD of a quadrant is read out at a time, while the four quadrants can be processed independently of each other. In standard imaging mode the CCDs are read out sequentially every 70 ms. Observations of point sources brighter than 1 mCrab will be effected by photon pile- up. However, special operating modes can be used to observe bright sources up to 150 mCrab in timing mode with 30 microseconds time resolution and very bright sources up to several crab in burst mode with 7 microseconds time resolution. We have tested one quadrant of the EPIC pn-CCD camera at line energies from 0.52 keV to 17.4 keV at the long beam test facility Panter in the focus of the qualification mirror module for XMM. In order to test the time resolution of the system, a mechanical chopper was used to periodically modulate the beam intensity. Pulse periods down to 0.7 ms were generated. This paper describes the performance of the pn-CCD detector in timing and burst readout modes with special emphasis on energy and time resolution.

A configurable distributed high-performance computing framework for satellite's TDI-CCD imaging simulation

NASA Astrophysics Data System (ADS)

Xue, Bo; Mao, Bingjing; Chen, Xiaomei; Ni, Guoqiang

2010-11-01

This paper renders a configurable distributed high performance computing(HPC) framework for TDI-CCD imaging simulation. It uses strategy pattern to adapt multi-algorithms. Thus, this framework help to decrease the simulation time with low expense. Imaging simulation for TDI-CCD mounted on satellite contains four processes: 1) atmosphere leads degradation, 2) optical system leads degradation, 3) electronic system of TDI-CCD leads degradation and re-sampling process, 4) data integration. Process 1) to 3) utilize diversity data-intensity algorithms such as FFT, convolution and LaGrange Interpol etc., which requires powerful CPU. Even uses Intel Xeon X5550 processor, regular series process method takes more than 30 hours for a simulation whose result image size is 1500 * 1462. With literature study, there isn't any mature distributing HPC framework in this field. Here we developed a distribute computing framework for TDI-CCD imaging simulation, which is based on WCF[1], uses Client/Server (C/S) layer and invokes the free CPU resources in LAN. The server pushes the process 1) to 3) tasks to those free computing capacity. Ultimately we rendered the HPC in low cost. In the computing experiment with 4 symmetric nodes and 1 server , this framework reduced about 74% simulation time. Adding more asymmetric nodes to the computing network, the time decreased namely. In conclusion, this framework could provide unlimited computation capacity in condition that the network and task management server are affordable. And this is the brand new HPC solution for TDI-CCD imaging simulation and similar applications.

Ion profiling in an ambient drift tube-ion mobility spectrometer using a high pixel density linear array detector IonCCD.

PubMed

Davila, Stephen J; Hadjar, Omar; Eiceman, Gary A

2013-07-16

A linear pixel-based detector array, the IonCCD, is characterized for use under ambient conditions with thermal (<1 eV) positive ions derived from purified air and a 10 mCi (63)Ni foil. The IonCCD combined with a drift tube-ion mobility spectrometer permitted the direct detection of gas phase ions at atmospheric pressure and confirmed a limit of detection of 3000 ions/pixel/frame established previously in both the keV (1-2 keV) and the hyper-thermal (10-40 eV) regimes. Results demonstrate the "broad-band" application of the IonCCD over 10(5) orders in ion energy and over 10(10) in operating pressure. The Faraday detector of a drift tube for an ion mobility spectrometer was replaced with the IonCCD providing images of ion profiles over the cross-section of the drift tube. Patterns in the ion profiles were developed in the drift tube cross-section by control of electric fields between wires of Bradbury Nielson and Tyndall Powell shutter designs at distances of 1-8 cm from the detector. Results showed that ion beams formed in wire sets, retained their shape with limited mixing by diffusion and Coulombic repulsion. Beam broadening determined as 95 μm/cm for hydrated protons in air with moisture of ~10 ppmv. These findings suggest a value of the IonCCD in further studies of ion motion and diffusion of thermalized ions, enhancing computational results from simulation programs, and in the design or operation of ion mobility spectrometers.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Glover, Karen; Li, Yue; Mukhopadhyay, Shreya

Beclin 1 (BECN1) is a key regulator of autophagy, a critical catabolic homeostasis pathway that involves sequestration of selected cytoplasmic components by multilayered vesicles called autophagosomes, followed by lysosomal fusion and degradation. BECN1 is a core component of class III phosphatidylinositol-3-kinase complexes responsible for autophagosome nucleation. Without heterologous binding partners, BECN1 forms an antiparallel homodimer via its coiled-coil domain (CCD). However, the last 16 CCD residues, composing an “overlap helix” (OH), have been crystallized in two mutually exclusive states: either as part of the CCD or packed against the C-terminal β-α repeated, autophagy-specific domain (BARAD). Here, using CD spectroscopy, isothermalmore » titration calorimetry, and small-angle X-ray scattering, we show that in the homodimeric state, the OH transitions between these two different packing states, with the predominant state comprising the OH packed against the BARAD, contrary to expectations based on known BECN1 interactions with heterologous partners. We confirmed this observation by comparing the impact of mutating four residues that mediate packing of the OH against both the CCD and BARAD on structure and stability of the CCD, the OH+BARAD, and the two-domain CCD–BARAD. Last, we used cellular assays to demonstrate that mutation of these OH-interface residues abrogates starvation-induced up-regulation of autophagy but does not affect basal autophagy. In summary, we have identified a BECN1 helical region that transitions between packing as part of either one of two conserved domains (i.e. the CCD or the BARAD). Our findings have important implications for the relative stability of autophagy-inactive and autophagy-active BECN1 complexes.« less

The Character and Formation of Elongated Depressions on the Upper Bulgarian Slope

NASA Astrophysics Data System (ADS)

Xu, Cuiling; Greinert, Jens; Haeckel, Matthias; Bialas, Jörg; Dimitrov, Lyubomir; Zhao, Guangtao

2018-06-01

Seafloor elongated depressions are indicators of gas seepage or slope instability. Here we report a sequence of slopeparallel elongated depressions that link to headwalls of sediment slides on upper slope. The depressions of about 250 m in width and several kilometers in length are areas of focused gas discharge indicated by bubble-release into the water column and methane enriched pore waters. Sparker seismic profiles running perpendicular and parallel to the coast, show gas migration pathways and trapped gas underneath these depressions with bright spots and seismic blanking. The data indicate that upward gas migration is the initial reason for fracturing sedimentary layers. In the top sediment where two young stages of landslides can be detected, the slopeparallel sediment weakening lengthens and deepens the surficial fractures, creating the elongated depressions in the seafloor supported by sediment erosion due to slope-parallel water currents.

Class network routing

DOEpatents

Bhanot, Gyan [Princeton, NJ; Blumrich, Matthias A [Ridgefield, CT; Chen, Dong [Croton On Hudson, NY; Coteus, Paul W [Yorktown Heights, NY; Gara, Alan G [Mount Kisco, NY; Giampapa, Mark E [Irvington, NY; Heidelberger, Philip [Cortlandt Manor, NY; Steinmacher-Burow, Burkhard D [Mount Kisco, NY; Takken, Todd E [Mount Kisco, NY; Vranas, Pavlos M [Bedford Hills, NY

2009-09-08

Class network routing is implemented in a network such as a computer network comprising a plurality of parallel compute processors at nodes thereof. Class network routing allows a compute processor to broadcast a message to a range (one or more) of other compute processors in the computer network, such as processors in a column or a row. Normally this type of operation requires a separate message to be sent to each processor. With class network routing pursuant to the invention, a single message is sufficient, which generally reduces the total number of messages in the network as well as the latency to do a broadcast. Class network routing is also applied to dense matrix inversion algorithms on distributed memory parallel supercomputers with hardware class function (multicast) capability. This is achieved by exploiting the fact that the communication patterns of dense matrix inversion can be served by hardware class functions, which results in faster execution times.

Chromatographic analysis of Polygalae Radix by online hyphenating pressurized liquid extraction

NASA Astrophysics Data System (ADS)

Song, Yuelin; Song, Qingqing; Li, Jun; Shi, Shepo; Guo, Liping; Zhao, Yunfang; Jiang, Yong; Tu, Pengfei

2016-06-01

Practicing “green analytical chemistry” is of great importance when profiling the chemical composition of complex matrices. Herein, a novel hybrid analytical platform was developed for direct chemical analysis of complex matrices by online hyphenating pressurized warm water extraction followed by turbulent flow chromatography coupled with high performance liquid chromatography-tandem mass spectrometry (PWWE-TFC-LC-MS/MS). Two parallel hollow guard columns acted as extraction vessels connected to a long narrow polyether ether ketone tube, while warm water served as extraction solvent and was delivered at a flow rate of 2.5 mL/min to generate considerable back pressure at either vessel. A column oven heated both the solvent and crude materials. A TFC column, which is advantageous for the comprehensive trapping of small molecular substances from fluids under turbulent flow conditions, was employed to transfer analytes from the PWWE module to LC-MS/MS. Two electronic valves alternated each vessel between extraction and elution phases. As a proof-of-concept, a famous herbal medicine for the treatment of neurodegenerative disorders, namely Polygalae Radix, was selected for the qualitative and quantitative analyses. The results suggest that the hybrid platform is advantageous in terms of decreasing time, material, and solvent consumption and in its automation, versatility, and environmental friendliness.

Experimental study of shock-accelerated inclined heavy gas cylinder

DOE PAGES

Olmstead, Dell; Wayne, Patrick; Yoo, Jae-Hwun; ...

2017-05-23

An experimental study examines shock acceleration with an initially diffuse cylindrical column of sulfur hexafluoride surrounded by air and inclined with respect to the shock front. Three-dimensional vorticity deposition produces flow patterns whose evolution is captured with planar laser-induced fluorescence in two planes. Both planes are thus parallel to the direction of the shock propagation. The first plane is vertical and passes through the axis of the column. The second visualization plane is normal to the first plane and passes through the centerline of the shock tube. Vortex formation in the vertical and centerline planes is initially characterized by differentmore » rates and morphologies due to differences in initial vorticity deposition. In the vertical plane, the vortex structure manifests a periodicity that varies with Mach number. The dominant wavelength in the vertical plane can be related to the geometry and compressibility of the initial conditions. At later times, the vortex interaction produces a complex and irregular three-dimensional pattern suggesting transition to turbulence. We present highly repeatable experimental data for Mach numbers 1.13, 1.4, 1.7, and 2.0 at column incline angles of 0, 20, and 30 degrees for about 50 nominal cylinder diameters (30 cm) of downstream travel.« less

Two-dimensional chromatographic analysis using three second-dimension columns for continuous comprehensive analysis of intact proteins.

PubMed

Zhu, Zaifang; Chen, Huang; Ren, Jiangtao; Lu, Juan J; Gu, Congying; Lynch, Kyle B; Wu, Si; Wang, Zhe; Cao, Chengxi; Liu, Shaorong

2018-03-01

We develop a new two-dimensional (2D) high performance liquid chromatography (HPLC) approach for intact protein analysis. Development of 2D HPLC has a bottleneck problem - limited second-dimension (second-D) separation speed. We solve this problem by incorporating multiple second-D columns to allow several second-D separations to be proceeded in parallel. To demonstrate the feasibility of using this approach for comprehensive protein analysis, we select ion-exchange chromatography as the first-dimension and reverse-phase chromatography as the second-D. We incorporate three second-D columns in an innovative way so that three reverse-phase separations can be performed simultaneously. We test this system for separating both standard proteins and E. coli lysates and achieve baseline resolutions for eleven standard proteins and obtain more than 500 peaks for E. coli lysates. This is an indication that the sample complexities are greatly reduced. We see less than 10 bands when each fraction of the second-D effluents are analyzed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE), compared to hundreds of SDS-PAGE bands as the original sample is analyzed. This approach could potentially be an excellent and general tool for protein analysis. Copyright © 2017 Elsevier B.V. All rights reserved.