MALDI-TOF mass spectrometry for quantitative gene expression analysis of acid responses in Staphylococcus aureus.

PubMed

Rode, Tone Mari; Berget, Ingunn; Langsrud, Solveig; Møretrø, Trond; Holck, Askild

2009-07-01

Microorganisms are constantly exposed to new and altered growth conditions, and respond by changing gene expression patterns. Several methods for studying gene expression exist. During the last decade, the analysis of microarrays has been one of the most common approaches applied for large scale gene expression studies. A relatively new method for gene expression analysis is MassARRAY, which combines real competitive-PCR and MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry. In contrast to microarray methods, MassARRAY technology is suitable for analysing a larger number of samples, though for a smaller set of genes. In this study we compare the results from MassARRAY with microarrays on gene expression responses of Staphylococcus aureus exposed to acid stress at pH 4.5. RNA isolated from the same stress experiments was analysed using both the MassARRAY and the microarray methods. The MassARRAY and microarray methods showed good correlation. Both MassARRAY and microarray estimated somewhat lower fold changes compared with quantitative real-time PCR (qRT-PCR). The results confirmed the up-regulation of the urease genes in acidic environments, and also indicated the importance of metal ion regulation. This study shows that the MassARRAY technology is suitable for gene expression analysis in prokaryotes, and has advantages when a set of genes is being analysed for an organism exposed to many different environmental conditions.

Colorimetric sensing of anions in water using ratiometric indicator-displacement assay.

PubMed

Feng, Liang; Li, Hui; Li, Xiao; Chen, Liang; Shen, Zheng; Guan, Yafeng

2012-09-19

The analysis of anions in water presents a difficult challenge due to their low charge-to-radius ratio, and the ability to discriminate among similar anions often remains problematic. The use of a 3×6 ratiometric indicator-displacement assay (RIDA) array for the colorimetric detection and identification of ten anions in water is reported. The sensor array consists of different combinations of colorimetric indicators and metal cations. The colorimetric indicators chelate with metal cations, forming the color changes. Upon the addition of anions, anions compete with the indicator ligands according to solubility product constants (K(sp)). The indicator-metal chelate compound changes color back dramatically when the competition of anions wins. The color changes of the RIDA array were used as a digital representation of the array response and analyzed with standard statistical methods, including principal component analysis and hierarchical clustering analysis. No confusion or errors in classification by hierarchical clustering analysis were observed in 44 trials. The limit of detection was calculated approximately, and most limits of detections of anions are well below μM level using our RIDA array. The pH effect, temperature influence, interfering anions were also investigated, and the RIDA array shows the feasibility of real sample testing. Copyright © 2012 Elsevier B.V. All rights reserved.

Genomic profiling of plasma cell disorders in a clinical setting: integration of microarray and FISH, after CD138 selection of bone marrow

PubMed Central

Berry, Nadine Kaye; Bain, Nicole L; Enjeti, Anoop K; Rowlings, Philip

2014-01-01

Aim To evaluate the role of whole genome comparative genomic hybridisation microarray (array-CGH) in detecting genomic imbalances as compared to conventional karyotype (GTG-analysis) or myeloma specific fluorescence in situ hybridisation (FISH) panel in a diagnostic setting for plasma cell dyscrasia (PCD). Methods A myeloma-specific interphase FISH (i-FISH) panel was carried out on CD138 PC-enriched bone marrow (BM) from 20 patients having BM biopsies for evaluation of PCD. Whole genome array-CGH was performed on reference (control) and neoplastic (test patient) genomic DNA extracted from CD138 PC-enriched BM and analysed. Results Comparison of techniques demonstrated a much higher detection rate of genomic imbalances using array-CGH. Genomic imbalances were detected in 1, 19 and 20 patients using GTG-analysis, i-FISH and array-CGH, respectively. Genomic rearrangements were detected in one patient using GTG-analysis and seven patients using i-FISH, while none were detected using array-CGH. I-FISH was the most sensitive method for detecting gene rearrangements and GTG-analysis was the least sensitive method overall. All copy number aberrations observed in GTG-analysis were detected using array-CGH and i-FISH. Conclusions We show that array-CGH performed on CD138-enriched PCs significantly improves the detection of clinically relevant and possibly novel genomic abnormalities in PCD, and thus could be considered as a standard diagnostic technique in combination with IGH rearrangement i-FISH. PMID:23969274

Genomic profiling of plasma cell disorders in a clinical setting: integration of microarray and FISH, after CD138 selection of bone marrow.

PubMed

Berry, Nadine Kaye; Bain, Nicole L; Enjeti, Anoop K; Rowlings, Philip

2014-01-01

To evaluate the role of whole genome comparative genomic hybridisation microarray (array-CGH) in detecting genomic imbalances as compared to conventional karyotype (GTG-analysis) or myeloma specific fluorescence in situ hybridisation (FISH) panel in a diagnostic setting for plasma cell dyscrasia (PCD). A myeloma-specific interphase FISH (i-FISH) panel was carried out on CD138 PC-enriched bone marrow (BM) from 20 patients having BM biopsies for evaluation of PCD. Whole genome array-CGH was performed on reference (control) and neoplastic (test patient) genomic DNA extracted from CD138 PC-enriched BM and analysed. Comparison of techniques demonstrated a much higher detection rate of genomic imbalances using array-CGH. Genomic imbalances were detected in 1, 19 and 20 patients using GTG-analysis, i-FISH and array-CGH, respectively. Genomic rearrangements were detected in one patient using GTG-analysis and seven patients using i-FISH, while none were detected using array-CGH. I-FISH was the most sensitive method for detecting gene rearrangements and GTG-analysis was the least sensitive method overall. All copy number aberrations observed in GTG-analysis were detected using array-CGH and i-FISH. We show that array-CGH performed on CD138-enriched PCs significantly improves the detection of clinically relevant and possibly novel genomic abnormalities in PCD, and thus could be considered as a standard diagnostic technique in combination with IGH rearrangement i-FISH.

Theory and investigation of acoustic multiple-input multiple-output systems based on spherical arrays in a room.

PubMed

Morgenstern, Hai; Rafaely, Boaz; Zotter, Franz

2015-11-01

Spatial attributes of room acoustics have been widely studied using microphone and loudspeaker arrays. However, systems that combine both arrays, referred to as multiple-input multiple-output (MIMO) systems, have only been studied to a limited degree in this context. These systems can potentially provide a powerful tool for room acoustics analysis due to the ability to simultaneously control both arrays. This paper offers a theoretical framework for the spatial analysis of enclosed sound fields using a MIMO system comprising spherical loudspeaker and microphone arrays. A system transfer function is formulated in matrix form for free-field conditions, and its properties are studied using tools from linear algebra. The system is shown to have unit-rank, regardless of the array types, and its singular vectors are related to the directions of arrival and radiation at the microphone and loudspeaker arrays, respectively. The formulation is then generalized to apply to rooms, using an image source method. In this case, the rank of the system is related to the number of significant reflections. The paper ends with simulation studies, which support the developed theory, and with an extensive reflection analysis of a room impulse response, using the platform of a MIMO system.

Design framework for spherical microphone and loudspeaker arrays in a multiple-input multiple-output system.

PubMed

Morgenstern, Hai; Rafaely, Boaz; Noisternig, Markus

2017-03-01

Spherical microphone arrays (SMAs) and spherical loudspeaker arrays (SLAs) facilitate the study of room acoustics due to the three-dimensional analysis they provide. More recently, systems that combine both arrays, referred to as multiple-input multiple-output (MIMO) systems, have been proposed due to the added spatial diversity they facilitate. The literature provides frameworks for designing SMAs and SLAs separately, including error analysis from which the operating frequency range (OFR) of an array is defined. However, such a framework does not exist for the joint design of a SMA and a SLA that comprise a MIMO system. This paper develops a design framework for MIMO systems based on a model that addresses errors and highlights the importance of a matched design. Expanding on a free-field assumption, errors are incorporated separately for each array and error bounds are defined, facilitating error analysis for the system. The dependency of the error bounds on the SLA and SMA parameters is studied and it is recommended that parameters should be chosen to assure matched OFRs of the arrays in MIMO system design. A design example is provided, demonstrating the superiority of a matched system over an unmatched system in the synthesis of directional room impulse responses.

Investigation of anodic TiO2 nanotube composition with high spatial resolution AES and ToF SIMS

NASA Astrophysics Data System (ADS)

Dronov, Alexey; Gavrilin, Ilya; Kirilenko, Elena; Dronova, Daria; Gavrilov, Sergey

2018-03-01

High resolution Scanning Auger Electron Spectroscopy (AES) and Time-of-Flight Secondary Ion Mass-Spectrometry (ToF SIMS) were used to investigate structure and elemental composition variation of both across an array of TiO2 nanotubes (NTs) and single tube of an array. The TiO2 NT array was grown by anodic oxidation of Ti foil in fluorine-containing ethylene glycol electrolyte. It was found that the studied anodic TiO2 nanotubes have a layered structure with rather sharp interfaces. The differences in AES depth profiling results of a single tube with the focused primary electron beam (point analysis) and over an area of 75 μm in diameter of a nanotube array with the defocused primary electron beam are discussed. Depth profiling by ToF SIMS was carried out over approximately the same size of a nanotube array to determine possible ionic fragments in the structure. The analysis results show that the combination of both mentioned methods is useful for a detailed analysis of nanostructures with complex morphology and multi-layered nature.

Characterization of transformation related genes in oral cancer cells.

PubMed

Chang, D D; Park, N H; Denny, C T; Nelson, S F; Pe, M

1998-04-16

A cDNA representational difference analysis (cDNA-RDA) and an arrayed filter technique were used to characterize transformation-related genes in oral cancer. From an initial comparison of normal oral epithelial cells and a human papilloma virus (HPV)-immortalized oral epithelial cell line, we obtained 384 differentially expressed gene fragments and arrayed them on a filter. Two hundred and twelve redundant clones were identified by three rounds of back hybridization. Sequence analysis of the remaining clones revealed 99 unique clones corresponding to 69 genes. The expression of these transformation related gene fragments in three nontumorigenic HPV-immortalized oral epithelial cell lines and three oral cancer cell lines were simultaneously monitored using a cDNA array hybridization. Although there was a considerable cell line-to-cell line variability in the expression of these clones, a reliable prediction of their expression could be made from the cDNA array hybridization. Our study demonstrates the utility of combining cDNA-RDA and arrayed filters in high-throughput gene expression difference analysis. The differentially expressed genes identified in this study should be informative in studying oral epithelial cell carcinogenesis.

SCARLET: Design of the Fresnel concentrator array for New Millennium Deep Space 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Murphy, D.M.; Eskenazi, M.I.

1997-12-31

The primary power for the JPL New Millennium Deep Space 1 spacecraft is a 2.6 kW concentrator solar array. This paper surveys the design and analysis employed to combine line-focus Fresnel lenses and multijunction (GaInP{sub 2}/GaAs/Ge) solar cells in the second-generation SCARLET (Solar Concentrator Array with Refractive Linear Element Technology) system. The array structure and mechanisms are reviewed. Discussion is focused on the lens and receiver, from the optimizations of optical efficiency and thermal management, to the design issues of environmental extremes, reliability, producibility, and control of pointing error.

A 16 element quasi-optical FET oscillator power combining array with external injection locking

NASA Astrophysics Data System (ADS)

Birkeland, Joel; Itoh, Tatsuo

1992-03-01

The authors present analysis, design and experimental results of a 16 element planar oscillator array for quasi-optical power combining. Each element in the array consists of a single FET oscillator with an input port for injection of the locking signal, and an output port which is connected to a patch radiator. The array is synchronized using a 16-way power dividing network which distributes the locking signal to the oscillating elements. The array is constructed using a two-sided microstrip configuration, with the oscillators and feed network on one side of a ground plane, and the patch radiators on the opposite side. An effective radiated power (ERP) of 28.2 W CW with an isotropic conversion gain of 9.9 dB was measured at 6 GHz. For an injected power of 10.3 dBm, a locking range of 453 MHz at a center frequency of 6.015 GHz was obtained; a bandwidth of 7.5 percent. Because of the simple nature of the individual oscillator elements, this approach is well suited to MMIC implementation.

ArrayInitiative - a tool that simplifies creating custom Affymetrix CDFs

PubMed Central

2011-01-01

Background Probes on a microarray represent a frozen view of a genome and are quickly outdated when new sequencing studies extend our knowledge, resulting in significant measurement error when analyzing any microarray experiment. There are several bioinformatics approaches to improve probe assignments, but without in-house programming expertise, standardizing these custom array specifications as a usable file (e.g. as Affymetrix CDFs) is difficult, owing mostly to the complexity of the specification file format. However, without correctly standardized files there is a significant barrier for testing competing analysis approaches since this file is one of the required inputs for many commonly used algorithms. The need to test combinations of probe assignments and analysis algorithms led us to develop ArrayInitiative, a tool for creating and managing custom array specifications. Results ArrayInitiative is a standalone, cross-platform, rich client desktop application for creating correctly formatted, custom versions of manufacturer-provided (default) array specifications, requiring only minimal knowledge of the array specification rules and file formats. Users can import default array specifications, import probe sequences for a default array specification, design and import a custom array specification, export any array specification to multiple output formats, export the probe sequences for any array specification and browse high-level information about the microarray, such as version and number of probes. The initial release of ArrayInitiative supports the Affymetrix 3' IVT expression arrays we currently analyze, but as an open source application, we hope that others will contribute modules for other platforms. Conclusions ArrayInitiative allows researchers to create new array specifications, in a standard format, based upon their own requirements. This makes it easier to test competing design and analysis strategies that depend on probe definitions. Since the custom array specifications are easily exported to the manufacturer's standard format, researchers can analyze these customized microarray experiments using established software tools, such as those available in Bioconductor. PMID:21548938

Infrared/microwave (IR/MW) micromirror array beam combiner design and analysis.

PubMed

Tian, Yi; Lv, Lijun; Jiang, Liwei; Wang, Xin; Li, Yanhong; Yu, Haiming; Feng, Xiaochen; Li, Qi; Zhang, Li; Li, Zhuo

2013-08-01

We investigated the design method of an infrared (IR)/microwave (MW) micromirror array type of beam combiner. The size of micromirror is in microscopic levels and comparable to MW wavelengths, so that the MW will not react in these dimensions, whereas the much shorter optical wavelengths will be reflected by them. Hence, the MW multilayered substrate was simplified and designed using transmission line theory. The beam combiner used an IR wavefront-division imaging technique to reflect the IR radiation image to the unit under test (UUT)'s pupil in a parallel light path. In addition, the boresight error detected by phase monopulse radar was analyzed using a moment-of method (MoM) and multilevel fast multipole method (MLFMM) acceleration technique. The boresight error introduced by the finite size of the beam combiner was less than 1°. Finally, in order to verify the wavefront-division imaging technique, a prototype of a micromirror array was fabricated, and IR images were tested. The IR images obtained by the thermal imager verified the correctness of the wavefront-division imaging technique.

Analysis and experimental demonstration of conformal adaptive phase-locked fiber array for laser communications and beam projection applications

NASA Astrophysics Data System (ADS)

Liu, Ling

The primary goal of this research is the analysis, development, and experimental demonstration of an adaptive phase-locked fiber array system for free-space optical communications and laser beam projection applications. To our knowledge, the developed adaptive phase-locked system composed of three fiber collimators (subapertures) with tip-tilt wavefront phase control at each subaperture represents the first reported fiber array system that implements both phase-locking control and adaptive wavefront tip-tilt control capabilities. This research has also resulted in the following innovations: (a) The first experimental demonstration of a phase-locked fiber array with tip-tilt wave-front aberration compensation at each fiber collimator; (b) Development and demonstration of the fastest currently reported stochastic parallel gradient descent (SPGD) system capable of operation at 180,000 iterations per second; (c) The first experimental demonstration of a laser communication link based on a phase-locked fiber array; (d) The first successful experimental demonstration of turbulence and jitter-induced phase distortion compensation in a phase-locked fiber array optical system; (e) The first demonstration of laser beam projection onto an extended target with a randomly rough surface using a conformal adaptive fiber array system. Fiber array optical systems, the subject of this study, can overcome some of the draw-backs of conventional monolithic large-aperture transmitter/receiver optical systems that are usually heavy, bulky, and expensive. The primary experimental challenges in the development of the adaptive phased-locked fiber-array included precise (<5 microrad) alignment of the fiber collimators and development of fast (100kHz-class) phase-locking and wavefront tip-tilt control systems. The precise alignment of the fiber collimator array is achieved through a specially developed initial coarse alignment tool based on high precision piezoelectric picomotors and a dynamic fine alignment mechanism implemented with specially designed and manufactured piezoelectric fiber positioners. Phase-locking of the fiber collimators is performed by controlling the phases of the output beams (beamlets) using integrated polarization-maintaining (PM) fiber-coupled LiNbO3 phase shifters. The developed phase-locking controllers are based on either the SPGD algorithm or the multi-dithering technique. Subaperture wavefront phase tip-tilt control is realized using piezoelectric fiber positioners that are controlled using a computer-based SPGD controller. Both coherent (phase-locked) and incoherent beam combining in the fiber array system are analyzed theoretically and experimentally. Two special fiber-based beam-combining testbeds have been built to demonstrate the technical feasibility of phase-locking compensation prior to free-space operation. In addition, the reciprocity of counter-propagating beams in a phase-locked fiber array system has been investigated. Coherent beam combining in a phase-locking system with wavefront phase tip-tilt compensation at each subaperture is successfully demonstrated when laboratory-simulated turbulence and wavefront jitters are present in the propagation path of the beamlets. In addition, coherent beam combining with a non-cooperative extended target in the control loop is successfully demonstrated.

Combined array CGH plus SNP genome analyses in a single assay for optimized clinical testing

PubMed Central

Wiszniewska, Joanna; Bi, Weimin; Shaw, Chad; Stankiewicz, Pawel; Kang, Sung-Hae L; Pursley, Amber N; Lalani, Seema; Hixson, Patricia; Gambin, Tomasz; Tsai, Chun-hui; Bock, Hans-Georg; Descartes, Maria; Probst, Frank J; Scaglia, Fernando; Beaudet, Arthur L; Lupski, James R; Eng, Christine; Wai Cheung, Sau; Bacino, Carlos; Patel, Ankita

2014-01-01

In clinical diagnostics, both array comparative genomic hybridization (array CGH) and single nucleotide polymorphism (SNP) genotyping have proven to be powerful genomic technologies utilized for the evaluation of developmental delay, multiple congenital anomalies, and neuropsychiatric disorders. Differences in the ability to resolve genomic changes between these arrays may constitute an implementation challenge for clinicians: which platform (SNP vs array CGH) might best detect the underlying genetic cause for the disease in the patient? While only SNP arrays enable the detection of copy number neutral regions of absence of heterozygosity (AOH), they have limited ability to detect single-exon copy number variants (CNVs) due to the distribution of SNPs across the genome. To provide comprehensive clinical testing for both CNVs and copy-neutral AOH, we enhanced our custom-designed high-resolution oligonucleotide array that has exon-targeted coverage of 1860 genes with 60 000 SNP probes, referred to as Chromosomal Microarray Analysis – Comprehensive (CMA-COMP). Of the 3240 cases evaluated by this array, clinically significant CNVs were detected in 445 cases including 21 cases with exonic events. In addition, 162 cases (5.0%) showed at least one AOH region >10 Mb. We demonstrate that even though this array has a lower density of SNP probes than other commercially available SNP arrays, it reliably detected AOH events >10 Mb as well as exonic CNVs beyond the detection limitations of SNP genotyping. Thus, combining SNP probes and exon-targeted array CGH into one platform provides clinically useful genetic screening in an efficient manner. PMID:23695279

Microbial whole‐cell arrays

PubMed Central

Elad, Tal; Lee, Jin Hyung; Belkin, Shimshon; Gu, Man Bock

2008-01-01

Summary The coming of age of whole‐cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines – the whole‐cell array. In the present review, we highlight the state‐of‐the‐art in the different disciplines essential for a functional bacterial array. These include the genetic engineering of the biological components, their immobilization in different polymers, technologies for live cell deposition and patterning on different types of solid surfaces, and cellular viability maintenance. Also reviewed are the types of signals emitted by the reporter cell arrays, some of the transduction methodologies for reading these signals and the mathematical approaches proposed for their analysis. Finally, we review some of the potential applications for bacterial cell arrays, and list the future needs for their maturation: a richer arsenal of high‐performance reporter strains, better methodologies for their incorporation into hardware platforms, design of appropriate detection circuits, the continuing development of dedicated algorithms for multiplex signal analysis and – most importantly – enhanced long‐term maintenance of viability and activity on the fabricated biochips. PMID:21261831

Analysis of differential gene expression by bead-based fiber-optic array in nonfunctioning pituitary adenomas.

PubMed

Jiang, Z; Gui, S; Zhang, Y

2011-05-01

Nonfunctioning pituitary adenomas (NFPAs) are relatively common, accounting for 30% of all pituitary adenomas; however, their pathogenesis remains enigmatic. To explore the possible pathogenesis of NFPAs, we used fiber-optic BeadArray to examine gene expression in 5 NFPAs compared with 3 normal pituitaries. 4 differentially expressed genes were chosen randomly for validation by reverse transcriptase-real time quantitative polymerase chain reaction (RT-qPCR). We then analyzed the differentially expressed gene profile with Kyoto Encyclopedia of Genes and Genomes (KEGG). The array analysis indentified significant increases in the expression of 1,402 genes and 383 expressed sequence tags (ESTs), and decreases in 1,697 genes and 113 ESTs in the NFPAs. Bioinformatic and pathway analysis showed that the genes HIGD1B, FAM5C, PMAIP1 and the pathway cell-cycle regulation may play an important role in tumorigenesis and progression of NFPAs. Our data suggest fiber-optic BeadArray combined with pathway analysis of differential gene expression profile appears to be a valid approach for investigating the pathogenesis of tumors. © Georg Thieme Verlag KG Stuttgart · New York.

GFZ Wireless Seismic Array (GFZ-WISE), a Wireless Mesh Network of Seismic Sensors: New Perspectives for Seismic Noise Array Investigations and Site Monitoring

PubMed Central

Picozzi, Matteo; Milkereit, Claus; Parolai, Stefano; Jaeckel, Karl-Heinz; Veit, Ingo; Fischer, Joachim; Zschau, Jochen

2010-01-01

Over the last few years, the analysis of seismic noise recorded by two dimensional arrays has been confirmed to be capable of deriving the subsoil shear-wave velocity structure down to several hundred meters depth. In fact, using just a few minutes of seismic noise recordings and combining this with the well known horizontal-to-vertical method, it has also been shown that it is possible to investigate the average one dimensional velocity structure below an array of stations in urban areas with a sufficient resolution to depths that would be prohibitive with active source array surveys, while in addition reducing the number of boreholes required to be drilled for site-effect analysis. However, the high cost of standard seismological instrumentation limits the number of sensors generally available for two-dimensional array measurements (i.e., of the order of 10), limiting the resolution in the estimated shear-wave velocity profiles. Therefore, new themes in site-effect estimation research by two-dimensional arrays involve the development and application of low-cost instrumentation, which potentially allows the performance of dense-array measurements, and the development of dedicated signal-analysis procedures for rapid and robust estimation of shear-wave velocity profiles. In this work, we present novel low-cost wireless instrumentation for dense two-dimensional ambient seismic noise array measurements that allows the real–time analysis of the surface-wavefield and the rapid estimation of the local shear-wave velocity structure for site response studies. We first introduce the general philosophy of the new system, as well as the hardware and software that forms the novel instrument, which we have tested in laboratory and field studies. PMID:22319298

Adaptive Injection-locking Oscillator Array for RF Spectrum Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Leung, Daniel

2011-04-19

A highly parallel radio frequency receiver using an array of injection-locking oscillators for on-chip, rapid estimation of signal amplitudes and frequencies is considered. The oscillators are tuned to different natural frequencies, and variable gain amplifiers are used to provide negative feedback to adapt the locking band-width with the input signal to yield a combined measure of input signal amplitude and frequency detuning. To further this effort, an array of 16 two-stage differential ring oscillators and 16 Gilbert-cell mixers is designed for 40-400 MHz operation. The injection-locking oscillator array is assembled on a custom printed-circuit board. Control and calibration is achievedmore » by on-board microcontroller.« less

An integrated analysis-synthesis array system for spatial sound fields.

PubMed

Bai, Mingsian R; Hua, Yi-Hsin; Kuo, Chia-Hao; Hsieh, Yu-Hao

2015-03-01

An integrated recording and reproduction array system for spatial audio is presented within a generic framework akin to the analysis-synthesis filterbanks in discrete time signal processing. In the analysis stage, a microphone array "encodes" the sound field by using the plane-wave decomposition. Direction of arrival of plane-wave components that comprise the sound field of interest are estimated by multiple signal classification. Next, the source signals are extracted by using a deconvolution procedure. In the synthesis stage, a loudspeaker array "decodes" the sound field by reconstructing the plane-wave components obtained in the analysis stage. This synthesis stage is carried out by pressure matching in the interior domain of the loudspeaker array. The deconvolution problem is solved by truncated singular value decomposition or convex optimization algorithms. For high-frequency reproduction that suffers from the spatial aliasing problem, vector panning is utilized. Listening tests are undertaken to evaluate the deconvolution method, vector panning, and a hybrid approach that combines both methods to cover frequency ranges below and above the spatial aliasing frequency. Localization and timbral attributes are considered in the subjective evaluation. The results show that the hybrid approach performs the best in overall preference. In addition, there is a trade-off between reproduction performance and the external radiation.

Hologic QDR 2000 whole-body scans: a comparison of three combinations of scan modes and analysis software

NASA Technical Reports Server (NTRS)

Spector, E.; LeBlanc, A.; Shackelford, L.

1995-01-01

This study reports on the short-term in vivo precision and absolute measurements of three combinations of whole-body scan modes and analysis software using a Hologic QDR 2000 dual-energy X-ray densitometer. A group of 21 normal, healthy volunteers (11 male and 10 female) were scanned six times, receiving one pencil-beam and one array whole-body scan on three occasions approximately 1 week apart. The following combinations of scan modes and analysis software were used: pencil-beam scans analyzed with Hologic's standard whole-body software (PB scans); the same pencil-beam analyzed with Hologic's newer "enhanced" software (EPB scans); and array scans analyzed with the enhanced software (EA scans). Precision values (% coefficient of variation, %CV) were calculated for whole-body and regional bone mineral content (BMC), bone mineral density (BMD), fat mass, lean mass, %fat and total mass. In general, there was no significant difference among the three scan types with respect to short-term precision of BMD and only slight differences in the precision of BMC. Precision of BMC and BMD for all three scan types was excellent: < 1% CV for whole-body values, with most regional values in the 1%-2% range. Pencil-beam scans demonstrated significantly better soft tissue precision than did array scans. Precision errors for whole-body lean mass were: 0.9% (PB), 1.1% (EPB) and 1.9% (EA). Precision errors for whole-body fat mass were: 1.7% (PB), 2.4% (EPB) and 5.6% (EA). EPB precision errors were slightly higher than PB precision errors for lean, fat and %fat measurements of all regions except the head, although these differences were significant only for the fat and % fat of the arms and legs. In addition EPB precision values exhibited greater individual variability than PB precision values. Finally, absolute values of bone and soft tissue were compared among the three combinations of scan and analysis modes. BMC, BMD, fat mass, %fat and lean mass were significantly different between PB scans and either of the EPB or EA scans. Differences were as large as 20%-25% for certain regional fat and BMD measurements. Additional work may be needed to examine the relative accuracy of the scan mode/software combinations and to identify reasons for the differences in soft tissue precision with the array whole-body scan mode.

Four-to-one power combiner for 20 GHz phased array antenna using RADC MMIC phase shifters

NASA Technical Reports Server (NTRS)

1991-01-01

The design and microwave simulation of two-to-one microstrip power combiners is described. The power combiners were designed for use in a four element phase array receive antenna subarray at 20 GHz. Four test circuits are described which were designed to enable testing of the power combiner and the four element phased array antenna. Test Circuit 1 enables measurement of the two-to-one power combiner. Test Circuit 2 enables measurement of the four-to-one power combiner. Test Circuit 3 enables measurement of a four element antenna array without phase shifting MMIC's in order to characterize the power combiner with the antenna patch-to-microstrip coaxial feedthroughs. Test circuit 4 is the four element phased array antenna including the RADC MMIC phase shifters and appropriate interconnects to provide bias voltages and control phase bits.

ArrayNinja: An Open Source Platform for Unified Planning and Analysis of Microarray Experiments.

PubMed

Dickson, B M; Cornett, E M; Ramjan, Z; Rothbart, S B

2016-01-01

Microarray-based proteomic platforms have emerged as valuable tools for studying various aspects of protein function, particularly in the field of chromatin biochemistry. Microarray technology itself is largely unrestricted in regard to printable material and platform design, and efficient multidimensional optimization of assay parameters requires fluidity in the design and analysis of custom print layouts. This motivates the need for streamlined software infrastructure that facilitates the combined planning and analysis of custom microarray experiments. To this end, we have developed ArrayNinja as a portable, open source, and interactive application that unifies the planning and visualization of microarray experiments and provides maximum flexibility to end users. Array experiments can be planned, stored to a private database, and merged with the imaged results for a level of data interaction and centralization that is not currently attainable with available microarray informatics tools. © 2016 Elsevier Inc. All rights reserved.

Analysis of the ICE combiner for multiple antenna arraying

NASA Technical Reports Server (NTRS)

Foster, C.; Marina, M.

1987-01-01

The passage of the International Cometary Explorer (ICE) through the tail of comet Giacobini-Zinner took place on September 11, 1985, at approximately 11:04 GMT. The signal-to-noise ratio of the data received from the ICE spacecraft during the comet encounter was improved by arraying the 64-m antenna channels A and B (RCP and LCP) with the two 34-m antennas. Specially designed combiners were built to combine the signals received by the three antennas at the different DSN sites to ensure that the spacecraft's weak signal was received. Although the ICE spacecraft was built with a 5-W transmitter and with a small antenna designed to provide data from no farther than 1 million miles, these combiners provided enough signal margin during the encounter to receive the ICE transmitted data from within the tail of comet Giacobini-Zinner, 44 million miles from earth.

Analysis of differential gene expression by bead-based fiber-optic array in growth-hormone-secreting pituitary adenomas.

PubMed

Jiang, Zhiquan; Gui, Songbo; Zhang, Yazhuo

2010-09-01

Growth-hormone-secreting pituitary adenomas (GHomas) account for approximately 20% of all pituitary neoplasms. However, the pathogenesis of GHomas remains to be elucidated. To explore the possible pathogenesis of GHomas, we used bead-based fiber-optic arrays to examine the gene expression in five GHomas and compared them to three healthy pituitaries. Four differentially expressed genes were chosen randomly for validation by quantitative real-time reverse transcription-polymerase chain reaction. We then performed pathway analysis on the identified differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes. Array analysis showed significant increases in the expression of 353 genes and 206 expressed sequence tags (ESTs) and decreases in 565 genes and 29 ESTs. Bioinformatic analysis showed that the genes HIGD1B, HOXB2, ANGPT2, HPGD and BTG2 may play an important role in the tumorigenesis and progression of GHomas. Pathway analysis showed that the wingless-type signaling pathway and extracellular-matrix receptor interactions may play a key role in the tumorigenesis and progression of GHomas. Our data suggested that there are numerous aberrantly expressed genes and pathways involved in the pathogenesis of GHomas. Bead-based fiber-optic arrays combined with pathway analysis of differentially expressed genes appear to be a valid method for investigating the pathogenesis of tumors.

Analysis of differential gene expression by bead-based fiber-optic array in growth-hormone-secreting pituitary adenomas

PubMed Central

JIANG, ZHIQUAN; GUI, SONGBO; ZHANG, YAZHUO

2010-01-01

Growth-hormone-secreting pituitary adenomas (GHomas) account for approximately 20% of all pituitary neoplasms. However, the pathogenesis of GHomas remains to be elucidated. To explore the possible pathogenesis of GHomas, we used bead-based fiber-optic arrays to examine the gene expression in five GHomas and compared them to three healthy pituitaries. Four differentially expressed genes were chosen randomly for validation by quantitative real-time reverse transcription-polymerase chain reaction. We then performed pathway analysis on the identified differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes. Array analysis showed significant increases in the expression of 353 genes and 206 expressed sequence tags (ESTs) and decreases in 565 genes and 29 ESTs. Bioinformatic analysis showed that the genes HIGD1B, HOXB2, ANGPT2, HPGD and BTG2 may play an important role in the tumorigenesis and progression of GHomas. Pathway analysis showed that the wingless-type signaling pathway and extracellular-matrix receptor interactions may play a key role in the tumorigenesis and progression of GHomas. Our data suggested that there are numerous aberrantly expressed genes and pathways involved in the pathogenesis of GHomas. Bead-based fiber-optic arrays combined with pathway analysis of differentially expressed genes appear to be a valid method for investigating the pathogenesis of tumors. PMID:22993617

Automated analysis of siRNA screens of cells infected by hepatitis C and dengue viruses based on immunofluorescence microscopy images

NASA Astrophysics Data System (ADS)

Matula, Petr; Kumar, Anil; Wörz, Ilka; Harder, Nathalie; Erfle, Holger; Bartenschlager, Ralf; Eils, Roland; Rohr, Karl

2008-03-01

We present an image analysis approach as part of a high-throughput microscopy siRNA-based screening system using cell arrays for the identification of cellular genes involved in hepatitis C and dengue virus replication. Our approach comprises: cell nucleus segmentation, quantification of virus replication level in the neighborhood of segmented cell nuclei, localization of regions with transfected cells, cell classification by infection status, and quality assessment of an experiment and single images. In particular, we propose a novel approach for the localization of regions of transfected cells within cell array images, which combines model-based circle fitting and grid fitting. By this scheme we integrate information from single cell array images and knowledge from the complete cell arrays. The approach is fully automatic and has been successfully applied to a large number of cell array images from screening experiments. The experimental results show a good agreement with the expected behaviour of positive as well as negative controls and encourage the application to screens from further high-throughput experiments.

A Topological Array Trigger for AGIS, the Advanced Gamma ray Imaging System

NASA Astrophysics Data System (ADS)

Krennrich, F.; Anderson, J.; Buckley, J.; Byrum, K.; Dawson, J.; Drake, G.; Haberichter, W.; Imran, A.; Krawczynski, H.; Kreps, A.; Schroedter, M.; Smith, A.

2008-12-01

Next generation ground based γ-ray observatories such as AGIS1 and CTA2 are expected to cover a 1 km2 area with 50-100 imaging atmospheric Cherenkov telescopes. The stereoscopic view ol air showers using multiple view points raises the possibility to use a topological array trigger that adds substantial flexibility, new background suppression capabilities and a reduced energy threshold. In this paper we report on the concept and technical implementation of a fast topological trigger system, that makes use of real time image processing of individual camera patterns and their combination in a stereoscopic array analysis. A prototype system is currently under construction and we discuss the design and hardware of this topological array trigger system.

Computer Drawing Method for Operating Characteristic Curve of PV Power Plant Array Unit

NASA Astrophysics Data System (ADS)

Tan, Jianbin

2018-02-01

According to the engineering design of large-scale grid-connected photovoltaic power stations and the research and development of many simulation and analysis systems, it is necessary to draw a good computer graphics of the operating characteristic curves of photovoltaic array elements and to propose a good segmentation non-linear interpolation algorithm. In the calculation method, Component performance parameters as the main design basis, the computer can get 5 PV module performances. At the same time, combined with the PV array series and parallel connection, the computer drawing of the performance curve of the PV array unit can be realized. At the same time, the specific data onto the module of PV development software can be calculated, and the good operation of PV array unit can be improved on practical application.

Advanced Power System Analysis Capabilities

NASA Technical Reports Server (NTRS)

1997-01-01

As a continuing effort to assist in the design and characterization of space power systems, the NASA Lewis Research Center's Power and Propulsion Office developed a powerful computerized analysis tool called System Power Analysis for Capability Evaluation (SPACE). This year, SPACE was used extensively in analyzing detailed operational timelines for the International Space Station (ISS) program. SPACE was developed to analyze the performance of space-based photovoltaic power systems such as that being developed for the ISS. It is a highly integrated tool that combines numerous factors in a single analysis, providing a comprehensive assessment of the power system's capability. Factors particularly critical to the ISS include the orientation of the solar arrays toward the Sun and the shadowing of the arrays by other portions of the station.

Tilted pillar array fabrication by the combination of proton beam writing and soft lithography for microfluidic cell capture Part 2: Image sequence analysis based evaluation and biological application.

PubMed

Járvás, Gábor; Varga, Tamás; Szigeti, Márton; Hajba, László; Fürjes, Péter; Rajta, István; Guttman, András

2018-02-01

As a continuation of our previously published work, this paper presents a detailed evaluation of a microfabricated cell capture device utilizing a doubly tilted micropillar array. The device was fabricated using a novel hybrid technology based on the combination of proton beam writing and conventional lithography techniques. Tilted pillars offer unique flow characteristics and support enhanced fluidic interaction for improved immunoaffinity based cell capture. The performance of the microdevice was evaluated by an image sequence analysis based in-house developed single-cell tracking system. Individual cell tracking allowed in-depth analysis of the cell-chip surface interaction mechanism from hydrodynamic point of view. Simulation results were validated by using the hybrid device and the optimized surface functionalization procedure. Finally, the cell capture capability of this new generation microdevice was demonstrated by efficiently arresting cells from a HT29 cell-line suspension. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

On a coherent investigation of the spectrum of cosmic rays in the energy range of 1014 - 1018 eV with KASCADE and KASCADE-Grande

NASA Astrophysics Data System (ADS)

Schoo, S.; Apel, W. D.; Arteaga-Velázquez, J. C.; Beck, K.; Bertaina, M.; Blümer, J.; Bozdog, H.; Brancus, I. M.; Cantoni, E.; Chiavassa, A.; Cossavella, F.; Daumiller, K.; de Souza, V.; Di Pierro, F.; Doll, P.; Engel, R.; Fuhrmann, D.; Gherghel-Lascu, A.; Gils, H. J.; Glasstetter, R.; Grupen, C.; Haungs, A.; Heck, D.; Hörandel, J. R.; Huber, D.; Huege, T.; Kampert, K. H.; Kang, D.; Klages, H. O.; Link, K.; Łuczak, P.; Mathes, H. J.; Mayer, H. J.; Milke, J.; Mitrica, B.; Morello, C.; Oehlschläger, J.; Ostapchenko, S.; Palmieri, N.; Petcu, M.; Pierog, T.; Rebel, H.; Roth, M.; Schieler, H.; Schröder, F. G.; Sima, O.; Toma, G.; Trinchero, G. C.; Ulrich, H.; Weindl, A.; Wochele, J.; Zabierowski, J.

2015-08-01

The KASCADE experiment and its extension KASCADE-Grande have significantly contributed to the current knowledge about the energy spectrum and composition of cosmic rays (CRs) with energies between the knee and the ankle. However, the data of both experiments were analysed separately, although Grande used the muon information of the KASCADE-array. A coherent analysis based on the combined data of both arrays is expected to profit from reconstructed shower observables with even higher accuracy compared to the stand-alone analyses. In addition, a significantly larger fiducial area is available. The aim of this analysis is to obtain the spectrum and composition of CRs in the range from 1014 to 1018 eV with a larger number of events and further reduced uncertainties using one unique reconstruction procedure for the entire energy range. This contribution will describe the motivation, the concept, and the current status of the combined analysis.

Split-plot microarray experiments: issues of design, power and sample size.

PubMed

Tsai, Pi-Wen; Lee, Mei-Ling Ting

2005-01-01

This article focuses on microarray experiments with two or more factors in which treatment combinations of the factors corresponding to the samples paired together onto arrays are not completely random. A main effect of one (or more) factor(s) is confounded with arrays (the experimental blocks). This is called a split-plot microarray experiment. We utilise an analysis of variance (ANOVA) model to assess differentially expressed genes for between-array and within-array comparisons that are generic under a split-plot microarray experiment. Instead of standard t- or F-test statistics that rely on mean square errors of the ANOVA model, we use a robust method, referred to as 'a pooled percentile estimator', to identify genes that are differentially expressed across different treatment conditions. We illustrate the design and analysis of split-plot microarray experiments based on a case application described by Jin et al. A brief discussion of power and sample size for split-plot microarray experiments is also presented.

Thermal management methods for compact high power LED arrays

NASA Astrophysics Data System (ADS)

Christensen, Adam; Ha, Minseok; Graham, Samuel

2007-09-01

The package and system level temperature distributions of a high power (>1W) light emitting diode (LED) array has been investigated using numerical heat flow models. For this analysis, a thermal resistor network model was combined with a 3D finite element submodel of an LED structure to predict system and die level temperatures. The impact of LED array density, LED power density, and active versus passive cooling methods on device operation were calculated. In order to help understand the role of various thermal resistances in cooling such compact arrays, the thermal resistance network was analyzed in order to estimate the contributions from materials as well as active and passive cooling schemes. An analysis of thermal stresses and residual stresses in the die are also calculated based on power dissipation and convection heat transfer coefficients. Results show that the thermal stress in the GaN layer are compressive which can impact the band gap and performance of the LEDs.

Using a whole-body 31P birdcage transmit coil and 16-element receive array for human cardiac metabolic imaging at 7T

PubMed Central

Dragonu, Iulius; Almujayyaz, Salam; Batzakis, Alex; Young, Liam A. J.; Purvis, Lucian A. B.; Clarke, William T.; Wichmann, Tobias; Lanz, Titus; Neubauer, Stefan; Robson, Matthew D.; Klomp, Dennis W. J.; Rodgers, Christopher T.

2017-01-01

Purpose Cardiac phosphorus magnetic resonance spectroscopy (31P-MRS) provides unique insight into the mechanisms of heart failure. Yet, clinical applications have been hindered by the restricted sensitivity of the surface radiofrequency-coils normally used. These permit the analysis of spectra only from the interventricular septum, or large volumes of myocardium, which may not be meaningful in focal disease. Löring et al. recently presented a prototype whole-body (52 cm diameter) transmit/receive birdcage coil for 31P at 7T. We now present a new, easily-removable, whole-body 31P transmit radiofrequency-coil built into a patient-bed extension combined with a 16-element receive array for cardiac 31P-MRS. Materials and methods A fully-removable (55 cm diameter) birdcage transmit coil was combined with a 16-element receive array on a Magnetom 7T scanner (Siemens, Germany). Electro-magnetic field simulations and phantom tests of the setup were performed. In vivo maps of B1+, metabolite signals, and saturation-band efficiency were acquired across the torsos of eight volunteers. Results The combined (volume-transmit, local receive array) setup increased signal-to-noise ratio 2.6-fold 10 cm below the array (depth of the interventricular septum) compared to using the birdcage coil in transceiver mode. The simulated coefficient of variation for B1+ of the whole-body coil across the heart was 46.7% (surface coil 129.0%); and the in vivo measured value was 38.4%. Metabolite images of 2,3-diphosphoglycerate clearly resolved the ventricular blood pools, and muscle tissue was visible in phosphocreatine (PCr) maps. Amplitude-modulated saturation bands achieved 71±4% suppression of phosphocreatine PCr in chest-wall muscles. Subjects reported they were comfortable. Conclusion This easy-to-assemble, volume-transmit, local receive array coil combination significantly improves the homogeneity and field-of-view for metabolic imaging of the human heart at 7T. PMID:29073228

Effects of correlated noise on the full-spectrum combining and complex-symbol combining arraying techniques

NASA Technical Reports Server (NTRS)

Vazirani, P.

1995-01-01

The process of combining telemetry signals received at multiple antennas, commonly referred to as arraying, can be used to improve communication link performance in the Deep Space Network (DSN). By coherently adding telemetry from multiple receiving sites, arraying produces an enhancement in signal-to-noise ratio (SNR) over that achievable with any single antenna in the array. A number of different techniques for arraying have been proposed and their performances analyzed in past literature. These analyses have compared different arraying schemes under the assumption that the signals contain additive white Gaussian noise (AWGN) and that the noise observed at distinct antennas is independent. In situations where an unwanted background body is visible to multiple antennas in the array, however, the assumption of independent noises is no longer applicable. A planet with significant radiation emissions in the frequency band of interest can be one such source of correlated noise. For example, during much of Galileo's tour of Jupiter, the planet will contribute significantly to the total system noise at various ground stations. This article analyzes the effects of correlated noise on two arraying schemes currently being considered for DSN applications: full-spectrum combining (FSC) and complex-symbol combining (CSC). A framework is presented for characterizing the correlated noise based on physical parameters, and the impact of the noise correlation on the array performance is assessed for each scheme.

A voxel visualization and analysis system based on AutoCAD

NASA Astrophysics Data System (ADS)

Marschallinger, Robert

1996-05-01

A collection of AutoLISP programs is presented which enable the visualization and analysis of voxel models by AutoCAD rel. 12/rel. 13. The programs serve as an interactive, graphical front end for manipulating the results of three-dimensional modeling software producing block estimation data. ASCII data files describing geometry and attributes per estimation block are imported and stored as a voxel array. Each voxel may contain multiple attributes, therefore different parameters may be incorporated in one voxel array. Voxel classification is implemented on a layer basis providing flexible treatment of voxel classes such as recoloring, peeling, or volumetry. A versatile clipping tool enables slicing voxel arrays according to combinations of three perpendicular clipping planes. The programs feature an up-to-date, graphical user interface for user-friendly operation by non AutoCAD specialists.

X ray photoelectron spectroscopy (XPS) analysis of Photosensitive ZrO2 array

NASA Astrophysics Data System (ADS)

Li, Y.; Zhao, G.; Zhu, R.; Kou, Z.

2018-03-01

Based on organic zirconium source as the starting material, by adding chemical modifiers which are made up with photosensitive ZrO2 sol. A uniformed ZrO2 array dot was fabricated with a mean diameter of around 800 nm. By using UV-vis spectra and X-ray photoelectron spectroscopy analysis method, studies the photosensitive ZrO2 gel film of photochemical reaction process and the photosensitive mechanism, to determine the zirconium atom centered chelate structure, reaction formed by metal chelate Zr atom for the center, and to establish the molecular model of the chelate. And studied the ultraviolet light in the process of the variation of the XPS spectra, Zr3d5/2 to 184.9 eV corresponding to the binding energy of the as the combination of state peak gradually reduce; By combining with the status of Zr-O peak gradually increase; The strength of the peak is gradually decline. This suggests that in the process of ultraviolet light photo chemical reaction happened. This study is of great significance to the micro fabrication of ZrO2 array not only to the memory devices but also to the optical devices.

UTOOLS: microcomputer software for spatial analysis and landscape visualization.

Treesearch

Alan A. Ager; Robert J. McGaughey

1997-01-01

UTOOLS is a collection of programs designed to integrate various spatial data in a way that allows versatile spatial analysis and visualization. The programs were designed for watershed-scale assessments in which a wide array of resource data must be integrated, analyzed, and interpreted. UTOOLS software combines raster, attribute, and vector data into "spatial...

Crust and Upper Mantle Structure Beneath Tibet and SW China From Seismic Tomography and Array Analysis

NASA Astrophysics Data System (ADS)

van der Hilst, R. D.; Li, C.; Yao, H.; Sun, R.; Meltzer, A. S.

2007-12-01

We will present a summary of the results of our seismological studies of crust and upper mantle heterogeneity and anisotropy beneath Tibet and SW China with data from temporary (PASSCAL) arrays as well as other regional, national, and global networks. In 2003 and 2004 MIT and CIGMR (Chengdu Institute of Geology and Mineral Resources) operated a 25 station array (3-component, broad band seismometers) in Sichuan and Yunnan provinces, SW China; during the same period Lehigh University (also in collaboration with CIGMR) operated a 75 station array in east Tibet. Data from these arrays allow delineation of mantle structure in unprecedented detail. We focus our presentation on results of two lines of seismological study. Travel time tomography (Li et al., PEPI, 2006; EPSL, 2007) with hand-picked phase arrivals from recordings at regional arrays, and combined with data from over 1,000 stations in China and with the global data base due to Engdahl et al. (BSSA, 1998), reveals substantial the structural complexity of the upper mantle beneath SE Asia. In particular, structures associated with subduction of the Indian plate beneath the Himalayas vary significantly from west Tibet (where the plate seems to have underthrusted the entire plateau) to east Tibet (where P-wave tomography provides no evidence for the presence of fast lithosphere beneath the Plateau proper). Further east, fast structures appear in the upper mantle transition zone, presumably related to stagnation of slab fragments associated with subduction of the Pacific plate. (2) Surface wave array tomography (Yao et al., GJI, 2006, 2007), using ambient noise interferometry and traditional (inter station) dispersion analysis, is used to delineate the 3-D structure of the crust and lithospheric mantle at length scales as small as 100 km beneath the MIT and Lehigh arrays. This analysis reveals a complex spatial distribution of intra-crustal low velocity zones (which may imply that crustal-scale faults influence the pattern of middle/lower crustal flow). We will also show preliminary results of surface wave inversion for azimuthal anisotropy, which - combined with previous results from shear wave splitting (Lev et al., EPSL, 2006) - give insight into the deformation of the upper mantle beneath the area under study.

Advanced Code-Division Multiplexers for Superconducting Detector Arrays

NASA Astrophysics Data System (ADS)

Irwin, K. D.; Cho, H. M.; Doriese, W. B.; Fowler, J. W.; Hilton, G. C.; Niemack, M. D.; Reintsema, C. D.; Schmidt, D. R.; Ullom, J. N.; Vale, L. R.

2012-06-01

Multiplexers based on the modulation of superconducting quantum interference devices are now regularly used in multi-kilopixel arrays of superconducting detectors for astrophysics, cosmology, and materials analysis. Over the next decade, much larger arrays will be needed. These larger arrays require new modulation techniques and compact multiplexer elements that fit within each pixel. We present a new in-focal-plane code-division multiplexer that provides multiplexing elements with the required scalability. This code-division multiplexer uses compact lithographic modulation elements that simultaneously multiplex both signal outputs and superconducting transition-edge sensor (TES) detector bias voltages. It eliminates the shunt resistor used to voltage bias TES detectors, greatly reduces power dissipation, allows different dc bias voltages for each TES, and makes all elements sufficiently compact to fit inside the detector pixel area. These in-focal plane code-division multiplexers can be combined with multi-GHz readout based on superconducting microresonators to scale to even larger arrays.

Combination of Vlbi, GPS and Slr Observations At The Observation Level For The Realization of Terrestrial and Celestial Reference Frames

NASA Astrophysics Data System (ADS)

Andersen, P. H.

Forsvarets forskningsinstitutt (FFI, the Norwegian Defence Research Establishment) has during the last 17 years developed a software system called GEOSAT, for the analysis of any type of high precision space geodetic observations. A unique feature of GEOSAT is the possibility of combining any combination of different space geode- tic data at the observation level with one consistent model and one consistent strategy. This is a much better strategy than the strategy in use today where different types of observations are processed separately using analysis software developed specifically for each technique. The results from each technique are finally combined a posteriori. In practice the models implemented in the software packages differ at the 1-cm level which is almost one order of magnitude larger than the internal precision of the most precise techniques. Another advantage of the new proposed combination method is that for example VLBI and GPS can use the same tropospheric model with common parameterization. The same is the case for the Earth orientation parameters, the geo- center coordinates and other geodetic or geophysical parameters where VLBI, GPS and SLR can have a common estimate for each of the parameters. The analysis with GEOSAT is automated for the combination of VLBI, SLR and GPS observations. The data are analyzed in batches of one day where the result from each daily arc is a SRIF array (Square Root Information Filter). A large number of SRIF arrays can be combined into a multi-year solution using the CSRIFS program (Com- bination Square Root Information Filter and Smoother). Four parameter levels are available and any parameter can, at each level, either be represented as a constant or a stochastic parameter (white noise, colored noise, or random walk). The batch length (i.e. the time interval between the addition of noise to the SRIF array) can be made time- and parameter dependent. GEOSAT and CSRIFS have been applied in the analysis of selected VLBI and SLR data (LAGEOS I &II) from the period January 1993 to July 2001. A selected number of arcs also include GPS data. Earth orientation parameters, geocenter motion, sta- tion coordinates and velocities were estimated simultaneously with the coordinates of the radio sources and satellite orbital parameters. Recent software improvements and 1 results of analyses will be presented at the meeting. 2

Coherent acoustic communication in a tidal estuary with busy shipping traffic.

PubMed

van Walree, Paul A; Neasham, Jeffrey A; Schrijver, Marco C

2007-12-01

High-rate acoustic communication experiments were conducted in a dynamic estuarine environment. Two current profilers deployed in a shipping lane were interfaced with acoustic modems, which modulated and transmitted the sensor readings every 200 s over a period of four days. QPSK modulation was employed at a raw data rate of 8 kbits on a 12-kHz carrier. Two 16-element hydrophone arrays, one horizontal and one vertical, were deployed near the shore. A multichannel decision-feedback equalizer was used to demodulate the modem signals received on both arrays. Long-term statistical analysis reveals the effects of the tidal cycle, subsea unit location, attenuation by the wake of passing vessels, and high levels of ship-generated noise on the fidelity of the communication links. The use of receiver arrays enables vast improvement in the overall reliability of data delivery compared with a single-receiver system, with performance depending strongly on array orientation. The vertical array offers the best performance overall, although the horizontal array proves more robust against shipping noise. Spatial coherence estimates, variation of array aperture, and inspection of array angular responses point to adaptive beamforming and coherent combining as the chief mechanisms of array gain.

Detection and Discrimination in One Pass Using the OPTEMA Towed Array

DTIC Science & Technology

2017-02-21

classification survey was performed using the One Pass Time domain EM Array (OPTEMA) at the former Southwestern Proving Ground near Hope, AR. Over...small arms ammunition, 20 to 155 mm projectiles, mortars, rockets, grenades, and up to 500-lb bombs [7]. Following the end of World War II, the proving...Range summary report [5]. It is assumed that combined field survey and analysis rates of $1000/acre for an EM -61 DGM survey and $30/anomaly for a

Nonlinear dynamics of solitary and optically injected two-element laser arrays with four different waveguide structures: a numerical study.

PubMed

Li, Nianqiang; Susanto, H; Cemlyn, B R; Henning, I D; Adams, M J

2018-02-19

We study the nonlinear dynamics of solitary and optically injected two-element laser arrays with a range of waveguide structures. The analysis is performed with a detailed direct numerical simulation, where high-resolution dynamic maps are generated to identify regions of dynamic instability in the parameter space of interest. Our combined one- and two-parameter bifurcation analysis uncovers globally diverse dynamical regimes (steady-state, oscillation, and chaos) in the solitary laser arrays, which are greatly influenced by static design waveguiding structures, the amplitude-phase coupling factor of the electric field, i.e. the linewidth-enhancement factor, as well as the control parameter, e.g. the pump rate. When external optical injection is introduced to one element of the arrays, we show that the whole system can be either injection-locked simultaneously or display rich, different dynamics outside the locking region. The effect of optical injection is to significantly modify the nature and the regions of nonlinear dynamics from those found in the solitary case. We also show similarities and differences (asymmetry) between the oscillation amplitude of the two elements of the array in specific well-defined regions, which hold for all the waveguiding structures considered. Our findings pave the way to a better understanding of dynamic instability in large arrays of lasers.

Shear wave velocities in the upper mantle of the Western Alps: new constraints using array analysis of seismic surface waves

NASA Astrophysics Data System (ADS)

Lyu, Chao; Pedersen, Helle A.; Paul, Anne; Zhao, Liang; Solarino, Stefano

2017-07-01

It remains challenging to obtain absolute shear wave velocities of heterogeneities of small lateral extension in the uppermost mantle. This study presents a cross-section of Vs across the strongly heterogeneous 3-D structure of the western European Alps, based on array analysis of data from 92 broad-band seismic stations from the CIFALPS experiment and from permanent networks in France and Italy. Half of the stations were located along a dense sublinear array. Using a combination of these stations and off-profile stations, fundamental-mode Rayleigh wave dispersion curves were calculated using a combined frequency-time beamforming approach. We calculated dispersion curves for seven arrays of approximately 100 km aperture and 14 arrays of approximately 50 km aperture, the latter with the aim of obtaining a 2-D vertical cross-section of Vs beneath the western Alps. The dispersion curves were inverted for Vs(z), with crustal interfaces imposed from a previous receiver function study. The array approach proved feasible, as Vs(z) from independent arrays vary smoothly across the profile length. Results from the seven large arrays show that the shear velocity of the upper mantle beneath the European plate is overall low compared to AK135 with the lowest velocities in the internal part of the western Alps, and higher velocities east of the Alps beneath the Po plain. The 2-D Vs model is coherent with (i) a ∼100 km thick eastward-dipping European lithosphere west of the Alps, (ii) very high velocities beneath the Po plain, coherent with the presence of the Alpine (European) slab and (iii) a narrow low-velocity anomaly beneath the core of the western Alps (from the Briançonnais to the Dora Maira massif), and approximately colocated with a similar anomaly observed in a recent teleseismic P-wave tomography. This intriguing anomaly is also supported by traveltime variations of subvertically propagating body waves from two teleseismic events that are approximately located on the profile great circle.

A composite sensor array impedentiometric electronic tongue Part II. Discrimination of basic tastes.

PubMed

Pioggia, G; Di Francesco, F; Marchetti, A; Ferro, M; Leardi, R; Ahluwalia, A

2007-05-15

An impedentiometric electronic tongue based on the combination of a composite sensor array and chemometric techniques aimed at the discrimination of soluble compounds able to elicit different gustative perceptions is presented. A composite array consisting of chemo-sensitive layers based on carbon nanotubes or carbon black dispersed in polymeric matrices and doped polythiophenes was used. The electrical impedance of the sensor array was measured at a frequency of 150 Hz by means of an impedance meter. The experimental set-up was designed in order to allow the automatic selection of a test solution and dipping of the sensor array following a dedicated measurement protocol. Measurements were carried out on 15 different solutions eliciting 5 different tastes (sodium chloride, citric acid, glucose, glutamic acid and sodium dehydrocholate for salty, sour, sweet, umami and bitter, respectively) at 3 concentration levels comprising the human perceptive range. In order to avoid over-fitting, more than 100 repetitions for each sample were carried in a 4-month period. Principal component analysis (PCA) was used to detect and remove outliers. Classification was performed by linear discriminant analysis (LDA). A fairly good degree of discrimination was obtained.

A blueprint for computational analysis of acoustical scattering from orchestral panel arrays

NASA Astrophysics Data System (ADS)

Burns, Thomas

2005-09-01

Orchestral panel arrays have been a topic of interest to acousticians, and it is reasonable to expect optimal design criteria to result from a combination of musician surveys, on-stage empirical data, and computational modeling of various configurations. Preparing a musicians survey to identify specific mechanisms of perception and sound quality is best suited for a clinically experienced hearing scientist. Measuring acoustical scattering from a panel array and discerning the effects from various boundaries is best suited for the experienced researcher in engineering acoustics. Analyzing a numerical model of the panel arrays is best suited for the tools typically used in computational engineering analysis. Toward this end, a streamlined process will be described using PROENGINEER to define a panel array geometry in 3-D, a commercial mesher to numerically discretize this geometry, SYSNOISE to solve the associated boundary element integral equations, and MATLAB to visualize the results. The model was run (background priority) on an SGI Altix (Linux) server with 12 CPUs, 24 Gbytes of RAM, and 1 Tbyte of disk space. These computational resources are available to research teams interested in this topic and willing to write and pursue grants.

Fingerprint analysis of Hibiscus mutabilis L. leaves based on ultra performance liquid chromatography with photodiode array detector combined with similarity analysis and hierarchical clustering analysis methods

PubMed Central

Liang, Xianrui; Ma, Meiling; Su, Weike

2013-01-01

Background: A method for chemical fingerprint analysis of Hibiscus mutabilis L. leaves was developed based on ultra performance liquid chromatography with photodiode array detector (UPLC-PAD) combined with similarity analysis (SA) and hierarchical clustering analysis (HCA). Materials and Methods: 10 batches of Hibiscus mutabilis L. leaves samples were collected from different regions of China. UPLC-PAD was employed to collect chemical fingerprints of Hibiscus mutabilis L. leaves. Results: The relative standard deviations (RSDs) of the relative retention times (RRT) and relative peak areas (RPA) of 10 characteristic peaks (one of them was identified as rutin) in precision, repeatability and stability test were less than 3%, and the method of fingerprint analysis was validated to be suitable for the Hibiscus mutabilis L. leaves. Conclusions: The chromatographic fingerprints showed abundant diversity of chemical constituents qualitatively in the 10 batches of Hibiscus mutabilis L. leaves samples from different locations by similarity analysis on basis of calculating the correlation coefficients between each two fingerprints. Moreover, the HCA method clustered the samples into four classes, and the HCA dendrogram showed the close or distant relations among the 10 samples, which was consistent to the SA result to some extent. PMID:23930008

A real-time signal combining system for Ka-band feed arrays using maximum-likelihood weight estimates

NASA Technical Reports Server (NTRS)

Vilnrotter, V. A.; Rodemich, E. R.

1990-01-01

A real-time digital signal combining system for use with Ka-band feed arrays is proposed. The combining system attempts to compensate for signal-to-noise ratio (SNR) loss resulting from antenna deformations induced by gravitational and atmospheric effects. The combining weights are obtained directly from the observed samples by using a sliding-window implementation of a vector maximum-likelihood parameter estimator. It is shown that with averaging times of about 0.1 second, combining loss for a seven-element array can be limited to about 0.1 dB in a realistic operational environment. This result suggests that the real-time combining system proposed here is capable of recovering virtually all of the signal power captured by the feed array, even in the presence of severe wind gusts and similar disturbances.

Computationally assisted screening and design of cell-interactive peptides by a cell-based assay using peptide arrays and a fuzzy neural network algorithm.

PubMed

Kaga, Chiaki; Okochi, Mina; Tomita, Yasuyuki; Kato, Ryuji; Honda, Hiroyuki

2008-03-01

We developed a method of effective peptide screening that combines experiments and computational analysis. The method is based on the concept that screening efficiency can be enhanced from even limited data by use of a model derived from computational analysis that serves as a guide to screening and combining the model with subsequent repeated experiments. Here we focus on cell-adhesion peptides as a model application of this peptide-screening strategy. Cell-adhesion peptides were screened by use of a cell-based assay of a peptide array. Starting with the screening data obtained from a limited, random 5-mer library (643 sequences), a rule regarding structural characteristics of cell-adhesion peptides was extracted by fuzzy neural network (FNN) analysis. According to this rule, peptides with unfavored residues in certain positions that led to inefficient binding were eliminated from the random sequences. In the restricted, second random library (273 sequences), the yield of cell-adhesion peptides having an adhesion rate more than 1.5-fold to that of the basal array support was significantly high (31%) compared with the unrestricted random library (20%). In the restricted third library (50 sequences), the yield of cell-adhesion peptides increased to 84%. We conclude that a repeated cycle of experiments screening limited numbers of peptides can be assisted by the rule-extracting feature of FNN.

Evaluation of global horizontal irradiance to plane-of-array irradiance models at locations across the United States

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lave, Matthew; Hayes, William; Pohl, Andrew

2015-02-02

We report an evaluation of the accuracy of combinations of models that estimate plane-of-array (POA) irradiance from measured global horizontal irradiance (GHI). This estimation involves two steps: 1) decomposition of GHI into direct and diffuse horizontal components and 2) transposition of direct and diffuse horizontal irradiance (DHI) to POA irradiance. Measured GHI and coincident measured POA irradiance from a variety of climates within the United States were used to evaluate combinations of decomposition and transposition models. A few locations also had DHI measurements, allowing for decoupled analysis of either the decomposition or the transposition models alone. Results suggest that decompositionmore » models had mean bias differences (modeled versus measured) that vary with climate. Transposition model mean bias differences depended more on the model than the location. Lastly, when only GHI measurements were available and combinations of decomposition and transposition models were considered, the smallest mean bias differences were typically found for combinations which included the Hay/Davies transposition model.« less

Design and optimization of a high-efficiency array generator in the mid-IR with binary subwavelength grooves.

PubMed

Bloom, Guillaume; Larat, Christian; Lallier, Eric; Lee-Bouhours, Mane-Si Laure; Loiseaux, Brigitte; Huignard, Jean-Pierre

2011-02-10

We have designed a high-efficiency array generator composed of subwavelength grooves etched in a GaAs substrate for operation at 4.5 μm. The method used combines rigorous coupled wave analysis with an optimization algorithm. The optimized beam splitter has both a high efficiency (∼96%) and a good intensity uniformity (∼0.2%). The fabrication error tolerances are numerically calculated, and it is shown that this subwavelength array generator could be fabricated with current electron beam writers and inductively coupled plasma etching. Finally, we studied the effect of a simple and realistic antireflection coating on the performance of the beam splitter.

Preparation and regulating cell adhesion of anion-exchangeable layered double hydroxide micropatterned arrays.

PubMed

Yao, Feng; Hu, Hao; Xu, Sailong; Huo, Ruijie; Zhao, Zhiping; Zhang, Fazhi; Xu, Fujian

2015-02-25

We describe a reliable preparation of MgAl-layered double hydroxide (MgAl-LDH) micropatterned arrays on gold substrate by combining SO3(-)-terminated self-assembly monolayer and photolithography. The synthesis route is readily extended to prepare LDH arrays on the SO3(-)-terminated polymer-bonded glass substrate amenable for cell imaging. The anion-exchangeable MgAl-LDH micropattern can act both as bioadhesive region for selective cell adhesion and as nanocarrier for drug molecules to regulate cell behaviors. Quantitative analysis of cell adhesion shows that selective HepG2 cell adhesion and spreading are promoted by the micropatterned MgAl-LDH, and also suppressed by methotrexate drug released from the LDH interlayer galleries.

Coherent and incoherent combination of Gaussian beams employing lens array distributed on the spherical chamber.

PubMed

Huang, Zhihua; Wei, Xiaofeng; Li, Mingzhong; Wang, Jianjun; Lin, Honghuan; Xu, Dangpeng; Deng, Ying; Zhang, Rui

2012-04-01

Coherent and incoherent combination of Gaussian beams employing a lens array distributed on the spherical chamber is theoretically analyzed. The output field of each source in the array is coupled through an individual optical system whose local optical axis coincides with the radial direction of the chamber. The resulting intensity profile near the origin is derived. The intensity profile and power in the bucket on the target for rectangular and hexagonal arrangement are numerically calculated. The influences of the center-to-center separation and the ring number of the focusing lens array are given. The synthetic intensity profile of incoherent combination changes little for a lens array scale much smaller than the chamber size. In contrast, the synthetic intensity profile of coherent combination shows an interference pattern with a sharp central peak and sidelobes.

The energy spectrum of ultra-high-energy cosmic rays measured by the Telescope Array FADC fluorescence detectors in monocular mode

NASA Astrophysics Data System (ADS)

Abu-Zayyad, T.; Aida, R.; Allen, M.; Anderson, R.; Azuma, R.; Barcikowski, E.; Belz, J. W.; Bergman, D. R.; Blake, S. A.; Cady, R.; Cheon, B. G.; Chiba, J.; Chikawa, M.; Cho, E. J.; Cho, W. R.; Fujii, H.; Fujii, T.; Fukuda, T.; Fukushima, M.; Hanlon, W.; Hayashi, K.; Hayashi, Y.; Hayashida, N.; Hibino, K.; Hiyama, K.; Honda, K.; Iguchi, T.; Ikeda, D.; Ikuta, K.; Inoue, N.; Ishii, T.; Ishimori, R.; Ito, H.; Ivanov, D.; Iwamoto, S.; Jui, C. C. H.; Kadota, K.; Kakimoto, F.; Kalashev, O.; Kanbe, T.; Kasahara, K.; Kawai, H.; Kawakami, S.; Kawana, S.; Kido, E.; Kim, H. B.; Kim, H. K.; Kim, J. H.; Kim, J. H.; Kitamoto, K.; Kitamura, S.; Kitamura, Y.; Kobayashi, K.; Kobayashi, Y.; Kondo, Y.; Kuramoto, K.; Kuzmin, V.; Kwon, Y. J.; Lan, J.; Lim, S. I.; Lundquist, J. P.; Machida, S.; Martens, K.; Matsuda, T.; Matsuura, T.; Matsuyama, T.; Matthews, J. N.; Myers, I.; Minamino, M.; Miyata, K.; Murano, Y.; Nagataki, S.; Nakamura, T.; Nam, S. W.; Nonaka, T.; Ogio, S.; Ogura, J.; Ohnishi, M.; Ohoka, H.; Oki, K.; Oku, D.; Okuda, T.; Ono, M.; Oshima, A.; Ozawa, S.; Park, I. H.; Pshirkov, M. S.; Rodriguez, D. C.; Roh, S. Y.; Rubtsov, G.; Ryu, D.; Sagawa, H.; Sakurai, N.; Sampson, A. L.; Scott, L. M.; Shah, P. D.; Shibata, F.; Shibata, T.; Shimodaira, H.; Shin, B. K.; Shin, J. I.; Shirahama, T.; Smith, J. D.; Sokolsky, P.; Sonley, T. J.; Springer, R. W.; Stokes, B. T.; Stratton, S. R.; Stroman, T. A.; Suzuki, S.; Takahashi, Y.; Takeda, M.; Taketa, A.; Takita, M.; Tameda, Y.; Tanaka, H.; Tanaka, K.; Tanaka, M.; Thomas, S. B.; Thomson, G. B.; Tinyakov, P.; Tkachev, I.; Tokuno, H.; Tomida, T.; Troitsky, S.; Tsunesada, Y.; Tsutsumi, K.; Tsuyuguchi, Y.; Uchihori, Y.; Udo, S.; Ukai, H.; Vasiloff, G.; Wada, Y.; Wong, T.; Yamakawa, Y.; Yamane, R.; Yamaoka, H.; Yamazaki, K.; Yang, J.; Yoneda, Y.; Yoshida, S.; Yoshii, H.; Zollinger, R.; Zundel, Z.

2013-08-01

We present a measurement of the energy spectrum of ultra-high-energy cosmic rays performed by the Telescope Array experiment using monocular observations from its two new FADC-based fluorescence detectors. After a short description of the experiment, we describe the data analysis and event reconstruction procedures. Since the aperture of the experiment must be calculated by Monte Carlo simulation, we describe this calculation and the comparisons of simulated and real data used to verify the validity of the aperture calculation. Finally, we present the energy spectrum calculated from the merged monocular data sets of the two FADC-based detectors, and also the combination of this merged spectrum with an independent, previously published monocular spectrum measurement performed by Telescope Array's third fluorescence detector [T. Abu-Zayyad et al., The energy spectrum of Telescope Array's middle drum detector and the direct comparison to the high resolution fly's eye experiment, Astroparticle Physics 39 (2012) 109-119, http://dx.doi.org/10.1016/j.astropartphys.2012.05.012, Available from: ]. This combined spectrum corroborates the recently published Telescope Array surface detector spectrum [T. Abu-Zayyad, et al., The cosmic-ray energy spectrum observed with the surface detector of the Telescope Array experiment, ApJ 768 (2013) L1, http://dx.doi.org/10.1088/2041-8205/768/1/L1, Available from: ] with independent systematic uncertainties.

Fabrication and optically pumped lasing of plasmonic nanolaser with regular ZnO/GaN nanoheterojunction array

NASA Astrophysics Data System (ADS)

Huang, Xiaoping; Zhang, Peifeng; Lin, En; Wang, Peng; Mei, Mingwei; Huang, Qiuying; Jiao, Jiao; Zhao, Qing

2017-09-01

We present the design and fabrication of a novel regularly arrayed plasmonic nanolasers. This main microstructure of the device is composed of a hexagonal array of n-ZnO/p-GaN nanoheterojunctions fabricated using the micro-fabrication method. Furthermore, the optically pumped lasing in the device is demonstrated. The spectroscopy characterization results of the device show that the surface plasmon excited around the NWs surface can be used to stimulate and strongly compress the optical modes in the NW cavity. This electromagnetic confinement effect is employed to optimize the beam quality and increase the light intensity compared to the laser fabricated with the bare NWs array. The impact of the array arrangement on the coherent combining efficiency of the arrayed nanolasers has been numerically studied. The results show that the arrayed hexagonal nanolasers could improve the combining efficiency compared to the nanolaser with the randomly positioned array. Qualitatively, these calculated results agree well with the experimental results of the laser beam spot mapping. This demonstrates the scope for using such architectures to improve the combination efficiency of the arrayed nanolasers.

Next generation miniature simultaneous multi-hyperspectral imaging systems

NASA Astrophysics Data System (ADS)

Hinnrichs, Michele; Gupta, Neelam

2014-03-01

The concept for a hyperspectral imaging system using a Fabry-Perot tunable filter (FPTF) array that is fabricated using "miniature optical electrical mechanical system" (MOEMS) technology. [1] Using an array of FPTF as an approach to hyperspectral imaging relaxes wavelength tuning requirements considerably because of the reduced portion of the spectrum that is covered by each element in the array. In this paper, Pacific Advanced Technology and ARL present the results of a concept design and performed analysis of a MOEMS based tunable Fabry-Perot array (FPTF) to perform simultaneous multispectral and hyperspectral imaging with relatively high spatial resolution. The concept design was developed with support of an Army SBIR Phase I program The Fabry-Perot tunable MOEMS filter array was combined with a miniature optics array and a focal plane array of 1024 x 1024 pixels to produce 16 colors every frame of the camera. Each color image has a spatial resolution of 256 x 256 pixels with an IFOV of 1.7 mrads and FOV of 25 degrees. The spectral images are collected simultaneously allowing high resolution spectral-spatial-temporal information in each frame of the camera, thus enabling the implementation of spectral-temporal-spatial algorithms in real-time to provide high sensitivity for the detection of weak signals in a high clutter background environment with low sensitivity to camera motion. The challenge in the design was the independent actuation of each Fabry Perot element in the array allowing for individual tuning. An additional challenge was the need to maximize the fill factor to improve the spatial coverage with minimal dead space. This paper will only address the concept design and analysis of the Fabry-Perot tunable filter array. A previous paper presented at SPIE DSS in 2012 explained the design of the optical array.

Near- and far-field infrasound monitoring in the Mediterranean area

NASA Astrophysics Data System (ADS)

Campus, Paola; Marchetti, Emanuele; Le Pichon, Alexis; Wallenstein, Nicolau; Ripepe, Maurizio; Kallel, Mohamed; Mialle, Pierrick

2013-04-01

The Mediterranean area is characterized by a number of very interesting sources of infrasound signals and offers a promising playground for the development of a deeper understanding of such sources and of the associated propagation models. The progress in the construction and certification of infrasound arrays belonging to the International Monitoring System (IMS) of the Comprehensive Nuclear-Test-Ban Treaty (CTBT) in the vicinity of this area has been complemented, in the last decade, by the construction of infrasound arrays established by several European research groups. The University of Florence (UniFi) plays a crucial role for the detection of infrasound signals in the Mediterranean area, having deployed since several years two infrasound arrays on Stromboli and Etna volcanoes, and, more recently, three infrasound arrays in the Alpine area of NW Italy and one infrasound array on the Apennines (Mount Amiata), designed and established in the framework of the ARISE Project. The IMS infrasound arrays IS42 (Graciosa, Azores, Portugal) and IS48 (Kesra, Tunisia) recorded, since the time of their certification, a number of far-field events which can be correlated with some near-field records of the infrasound arrays belonging to UniFi. An analysis of the results and potentialities of infrasound source's detections in near and far-field realized by IS42, IS48 and UniFi arrays in the Mediterranean area, with special focus on volcanic events is presented. The combined results deriving from the analysis of data recorded by the Unifi arrays and by the IS42 and IS48 arrays, in collaboration with the Department of Analyse et Surveillance (CEA/DASE), will generate a synergy which will certainly contribute to the progress of the ARISE Project.

Experimental demonstration of passive coherent combining of fiber lasers by phase contrast filtering.

PubMed

Jeux, François; Desfarges-Berthelemot, Agnès; Kermène, Vincent; Barthelemy, Alain

2012-12-17

We report experiments on a new laser architecture involving phase contrast filtering to coherently combine an array of fiber lasers. We demonstrate that the new technique yields a more stable phase-locking than standard methods using only amplitude filtering. A spectral analysis of the output beams shows that the new scheme generates more resonant frequencies common to the coupled lasers. This property can enhance the combining efficiency when the number of lasers to be coupled is large.

Infrasonic and seismic signals from earthquakes and explosions observed with Plostina seismo-acoustic array

NASA Astrophysics Data System (ADS)

Ghica, D.; Ionescu, C.

2012-04-01

Plostina seismo-acoustic array has been recently deployed by the National Institute for Earth Physics in the central part of Romania, near the Vrancea epicentral area. The array has a 2.5 km aperture and consists of 7 seismic sites (PLOR) and 7 collocated infrasound instruments (IPLOR). The array is being used to assess the importance of collocated seismic and acoustic sensors for the purposes of (1) seismic monitoring of the local and regional events, and (2) acoustic measurement, consisting of detection of the infrasound events (explosions, mine and quarry blasts, earthquakes, aircraft etc.). This paper focuses on characterization of infrasonic and seismic signals from the earthquakes and explosions (accidental and mining type). Two Vrancea earthquakes with magnitude above 5.0 were selected to this study: one occurred on 1st of May 2011 (MD = 5.3, h = 146 km), and the other one, on 4th October 2011 (MD = 5.2, h = 142 km). The infrasonic signals from the earthquakes have the appearance of the vertical component of seismic signals. Because the mechanism of the infrasonic wave formation is the coupling of seismic waves with the atmosphere, trace velocity values for such signals are compatible with the characteristics of the various seismic phases observed with PLOR array. The study evaluates and characterizes, as well, infrasound and seismic data recorded from the explosion caused by the military accident produced at Evangelos Florakis Naval Base, in Cyprus, on 11th July 2011. Additionally, seismo-acoustic signals presumed to be related to strong mine and quarry blasts were investigated. Ground truth of mine observations provides validation of this interpretation. The combined seismo-acoustic analysis uses two types of detectors for signal identification: one is the automatic detector DFX-PMCC, applied for infrasound detection and characterization, while the other one, which is used for seismic data, is based on array processing techniques (beamforming and frequency-wave number analysis). Spectrograms of the recorded infrasonic and seismic data were examined, showing that an earthquake produces acoustic signals with a high energy in the 1 to 5 Hz frequency range, while, for the explosion, this range lays below 0.6 Hz. Using the combined analysis of the seismic and acoustic data, Plostina array can greatly enhance the event detection and localization in the region. The analysis can be, as well, particularly important in identifying sources of industrial explosion, and therefore, in monitoring of the hazard created both by earthquakes and anthropogenic sources of pollution (chemical factories, nuclear and power plants, refineries, mines).

Facile synthesis of silicon nanowire-nanopillar superhydrophobic structures

NASA Astrophysics Data System (ADS)

Roy, Abhijit; Satpati, Biswarup

2018-04-01

We have used metal assisted chemical etching (MACE) method to produce silicon (Si) nanowire-nanopillar array. Nanowire-nanopillar combined structures show higher degree of hydrophobicity compared to its nanowire (Si-NW) counterparts. The rate of etching is depended on initial metal deposition. The structural analysis was carried out using scanning electron microscopy (SEM) in combination with transmission electron microscopy (TEM) to determine different parameters like etching direction, crystallinity etc.

Modular Analytical Multicomponent Analysis in Gas Sensor Aarrays

PubMed Central

Chaiyboun, Ali; Traute, Rüdiger; Kiesewetter, Olaf; Ahlers, Simon; Müller, Gerhard; Doll, Theodor

2006-01-01

A multi-sensor system is a chemical sensor system which quantitatively and qualitatively records gases with a combination of cross-sensitive gas sensor arrays and pattern recognition software. This paper addresses the issue of data analysis for identification of gases in a gas sensor array. We introduce a software tool for gas sensor array configuration and simulation. It concerns thereby about a modular software package for the acquisition of data of different sensors. A signal evaluation algorithm referred to as matrix method was used specifically for the software tool. This matrix method computes the gas concentrations from the signals of a sensor array. The software tool was used for the simulation of an array of five sensors to determine gas concentration of CH4, NH3, H2, CO and C2H5OH. The results of the present simulated sensor array indicate that the software tool is capable of the following: (a) identify a gas independently of its concentration; (b) estimate the concentration of the gas, even if the system was not previously exposed to this concentration; (c) tell when a gas concentration exceeds a certain value. A gas sensor data base was build for the configuration of the software. With the data base one can create, generate and manage scenarios and source files for the simulation. With the gas sensor data base and the simulation software an on-line Web-based version was developed, with which the user can configure and simulate sensor arrays on-line.

Economic analysis of the unified heliostat array. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1981-01-31

The Unified Heliostat Array (UHA) was investigated as to cost and optical performance. Two heliostats, the Veda Industrial Heliostat (VIH) and the Repowering Heliostat were investigated in conjunction with the UHA. The UHA was found to be a viable candidate for solar thermal central receiver applications. The UHA-VIH combination was shown to provide very high flux densities and to be suitable for high temperature applications in the 1000/sup 0/K to 2000/sup 0/K range. These temperatures were shown to be achievable even with very small (1 MWt) collector fields.

Optical Path Difference Fluctations at the CHARA Interferometric Array

NASA Astrophysics Data System (ADS)

Merand, A.; ten Brummelaar, T. A.; McAlister, H. A.; Ridgway, S. T.; Sturmann, J.; Sturmann, L.; Turner, N. H.; Bagnuolo, W. G.; Hrynevych, M.; Shure, M. A.

2001-05-01

Commissioning observations at the CHARA Array have been carried out with the two south telescopes, with a telescope separation of 34 meters. Due to the size of the array (>340 meters across) and the optical delay geometry, the beams travel horizontal distances of approximately 200 meters, with a number of reflections in the telescope coude area and the optical delay and beam combination areas. Stellar and laboratory observations have been analyzed to determine the variations of the optical path, as revealed by shifts in the interference pattern. The power spectra of the OPD variations are diagnostic of the atmospheric turbulence characteristics, and of any internal vibrations in the laboratory. Results of the OPD analysis will be compared to similar studies at other interferometric facilities. The CHARA Array, a six-telescope O/IR interferometric array operated by Georgia State University on Mt. Wilson, Calfornia, was funded by the National Science Foundation, the W.M. Keck Foundation, the David and Lucile Packard Foundation, and Georgia State University.

Spacecraft Leak Location Using Structure-Borne Noise

NASA Astrophysics Data System (ADS)

Reusser, R. S.; Chimenti, D. E.; Holland, S. D.; Roberts, R. A.

2010-02-01

Guided ultrasonic waves, generated by air escaping through a small hole, have been measured with an 8×8 piezoelectric phased-array detector. Rapid location of air leaks in a spacecraft skin, caused by high-speed collisions with small objects, is essential for astronaut survival. Cross correlation of all 64 elements, one pair at a time, on a diced PZT disc combined with synthetic aperture analysis determines the dominant direction of wave propagation. The leak location is triangulated by combining data from two or more detector. To optimize the frequency band selection for the most robust direction finding, noise-field measurements of a plate with integral stiffeners have been performed using laser Doppler velocimetry. We compare optical and acoustic measurements to analyze the influence of the PZT array detector and its mechanical coupling to the plate.

Particle-Based Microarrays of Oligonucleotides and Oligopeptides.

PubMed

Nesterov-Mueller, Alexander; Maerkle, Frieder; Hahn, Lothar; Foertsch, Tobias; Schillo, Sebastian; Bykovskaya, Valentina; Sedlmayr, Martyna; Weber, Laura K; Ridder, Barbara; Soehindrijo, Miriam; Muenster, Bastian; Striffler, Jakob; Bischoff, F Ralf; Breitling, Frank; Loeffler, Felix F

2014-10-28

In this review, we describe different methods of microarray fabrication based on the use of micro-particles/-beads and point out future tendencies in the development of particle-based arrays. First, we consider oligonucleotide bead arrays, where each bead is a carrier of one specific sequence of oligonucleotides. This bead-based array approach, appearing in the late 1990s, enabled high-throughput oligonucleotide analysis and had a large impact on genome research. Furthermore, we consider particle-based peptide array fabrication using combinatorial chemistry. In this approach, particles can directly participate in both the synthesis and the transfer of synthesized combinatorial molecules to a substrate. Subsequently, we describe in more detail the synthesis of peptide arrays with amino acid polymer particles, which imbed the amino acids inside their polymer matrix. By heating these particles, the polymer matrix is transformed into a highly viscous gel, and thereby, imbedded monomers are allowed to participate in the coupling reaction. Finally, we focus on combinatorial laser fusing of particles for the synthesis of high-density peptide arrays. This method combines the advantages of particles and combinatorial lithographic approaches.

Particle-Based Microarrays of Oligonucleotides and Oligopeptides

PubMed Central

Nesterov-Mueller, Alexander; Maerkle, Frieder; Hahn, Lothar; Foertsch, Tobias; Schillo, Sebastian; Bykovskaya, Valentina; Sedlmayr, Martyna; Weber, Laura K.; Ridder, Barbara; Soehindrijo, Miriam; Muenster, Bastian; Striffler, Jakob; Bischoff, F. Ralf; Breitling, Frank; Loeffler, Felix F.

2014-01-01

In this review, we describe different methods of microarray fabrication based on the use of micro-particles/-beads and point out future tendencies in the development of particle-based arrays. First, we consider oligonucleotide bead arrays, where each bead is a carrier of one specific sequence of oligonucleotides. This bead-based array approach, appearing in the late 1990s, enabled high-throughput oligonucleotide analysis and had a large impact on genome research. Furthermore, we consider particle-based peptide array fabrication using combinatorial chemistry. In this approach, particles can directly participate in both the synthesis and the transfer of synthesized combinatorial molecules to a substrate. Subsequently, we describe in more detail the synthesis of peptide arrays with amino acid polymer particles, which imbed the amino acids inside their polymer matrix. By heating these particles, the polymer matrix is transformed into a highly viscous gel, and thereby, imbedded monomers are allowed to participate in the coupling reaction. Finally, we focus on combinatorial laser fusing of particles for the synthesis of high-density peptide arrays. This method combines the advantages of particles and combinatorial lithographic approaches. PMID:27600347

Economic analysis of the unified heliostat array

NASA Astrophysics Data System (ADS)

1980-11-01

The array (UHA) is comprised of conventional two-axis heliostats mounted on a terraced south-facing wall of a single structure. The arrangement of heliostats on the array is chosen to eliminate or control the degree of inter-heliostat shading and blocking. The UHA was investigated as to cost and optical performance. Two heliostats, the Veda Industrial Heliostat (VIH) and the Repowering Helistat were investigated in conjunction with the UHA. The UHA is found to be a viable candidate for solar thermal central receiver applications. The UHA-VIH combination was shown to provide very high flux densities and to be suitable for high temperature applications in the 1000 K to 2000 K range. These temperatures were shown to be achieve even with very small (1 MWt) collector fields.

A Distributed Amplifier System for Bilayer Lipid Membrane (BLM) Arrays With Noise and Individual Offset Cancellation.

PubMed

Crescentini, Marco; Thei, Frederico; Bennati, Marco; Saha, Shimul; de Planque, Maurits R R; Morgan, Hywel; Tartagni, Marco

2015-06-01

Lipid bilayer membrane (BLM) arrays are required for high throughput analysis, for example drug screening or advanced DNA sequencing. Complex microfluidic devices are being developed but these are restricted in terms of array size and structure or have integrated electronic sensing with limited noise performance. We present a compact and scalable multichannel electrophysiology platform based on a hybrid approach that combines integrated state-of-the-art microelectronics with low-cost disposable fluidics providing a platform for high-quality parallel single ion channel recording. Specifically, we have developed a new integrated circuit amplifier based on a novel noise cancellation scheme that eliminates flicker noise derived from devices under test and amplifiers. The system is demonstrated through the simultaneous recording of ion channel activity from eight bilayer membranes. The platform is scalable and could be extended to much larger array sizes, limited only by electronic data decimation and communication capabilities.

A microfluidic cigarette smoke collecting platform for simultaneous sample extraction and multiplex analysis.

PubMed

Hu, Shan-Wen; Xu, Bi-Yi; Qiao, Shu; Zhao, Ge; Xu, Jing-Juan; Chen, Hong-Yuan; Xie, Fu-Wei

2016-04-01

In this work, we report a novel microfluidic gas collecting platform aiming at simultaneous sample extraction and multiplex mass spectrometry (MS) analysis. An alveolar-mimicking elastic polydimethylsiloxane (PDMS) structures was designed to move dynamically driven by external pressure. The movement was well tuned both by its amplitude and rhythm following the natural process of human respiration. By integrating the alveolar units into arrays and assembling them to gas channels, a cyclic contraction/expansion system for gas inhale and exhale was successfully constructed. Upon equipping this system with a droplet array on the alveolar array surface, we were able to get information of inhaled smoke in a new strategy. Here, with cigarette smoke as an example, analysis of accumulation for target molecules during passive smoking is taken. Relationships between the breathing times, distances away from smokers and inhaled content of nicotine are clarified. Further, by applying different types of extraction solvent droplets on different locations of the droplet array, simultaneous extraction of nicotine, formaldehyde and caproic acid in sidestream smoke (SS) are realized. Since the extract droplets are spatially separated, they can be directly analyzed by MS which is fast and can rid us of all complex sample separation and purification steps. Combining all these merits, this small, cheap and portable platform might find wide application in inhaled air pollutant analysis both in and outdoors. Copyright © 2015 Elsevier B.V. All rights reserved.

Modeling Intracochlear Magnetic Stimulation: A Finite-Element Analysis.

PubMed

Mukesh, S; Blake, D T; McKinnon, B J; Bhatti, P T

2017-08-01

This study models induced electric fields, and their gradient, produced by pulsatile current stimulation of submillimeter inductors for cochlear implantation. Using finite-element analysis, the lower chamber of the cochlea, scala tympani, is modeled as a cylindrical structure filled with perilymph bounded by tissue, bone, and cochlear neural elements. Single inductors as well as an array of inductors are modeled. The coil strength (~100 nH) and excitation parameters (peak current of 1-5 A, voltages of 16-20 V) are based on a formative feasibility study conducted by our group. In that study, intracochlear micromagnetic stimulation achieved auditory activation as measured through the auditory brainstem response in a feline model. With respect to the finite element simulations, axial symmetry of the inductor geometry is exploited to improve computation time. It is verified that the inductor coil orientation greatly affects the strength of the induced electric field and thereby the ability to affect the transmembrane potential of nearby neural elements. Furthermore, upon comparing an array of micro-inductors with a typical multi-site electrode array, magnetically excited arrays retain greater focus in terms of the gradient of induced electric fields. Once combined with further in vivo analysis, this modeling study may enable further exploration of the mechanism of magnetically induced, and focused neural stimulation.

Magnetomicrofluidics Circuits for Organizing Bioparticle Arrays

NASA Astrophysics Data System (ADS)

Abedini-Nassab, Roozbeh

Single-cell analysis (SCA) tools have important applications in the analysis of phenotypic heterogeneity, which is difficult or impossible to analyze in bulk cell culture or patient samples. SCA tools thus have a myriad of applications ranging from better credentialing of drug therapies to the analysis of rare latent cells harboring HIV infection or in Cancer. However, existing SCA systems usually lack the required combination of programmability, flexibility, and scalability necessary to enable the study of cell behaviors and cell-cell interactions at the scales sufficient to analyze extremely rare events. To advance the field, I have developed a novel, programmable, and massively-parallel SCA tool which is based on the principles of computer circuits. By integrating these magnetic circuits with microfluidics channels, I developed a platform that can organize a large number of single particles into an array in a controlled manner. My magnetophoretic circuits use passive elements constructed in patterned magnetic thin films to move cells along programmed tracks with an external rotating magnetic field. Cell motion along these tracks is analogous to the motion of charges in an electrical conductor, following a rule similar to Ohm's law. I have also developed asymmetric conductors, similar to electrical diodes, and storage sites for cells that behave similarly to electrical capacitors. I have also developed magnetophoretic circuits which use an overlaid pattern of microwires to switch single cells between different tracks. This switching mechanism, analogous to the operation of electronic transistors, is achieved by establishing a semiconducting gap in the magnetic pattern which can be changed from an insulating state to a conducting state by application of electrical current to an overlaid electrode. I performed an extensive study on the operation of transistors to optimize their geometry and minimize the required gate currents. By combining these elements into integrated circuits, I have built devices which are capable of organizing a precise number of cells into individually addressable array sites, similar to how a random access memory (RAM) stores electronic data. My programmable magnetic circuits allow for the organization of both cells and single-cell pairs into large arrays. Single cells can also potentially be retrieved for downstream high-throughput genomic analysis. In order to enhance the efficiency of the tool and to increase the delivery speed of the particles, I have also developed microfluidics systems that are combined with the magnetophoretic circuits. This hybrid system, called magnetomicrofluidics, is capable of rapidly organizing an array of particles and cells with the high precision and control. I have also shown that cells can be grown inside these chips for multiple days, enabling the long-term phenotypic analysis of rare cellular events. These types of studies can reveal important insights about the intercellular signaling networks and answer crucial questions in biology and immunology.

Comparison of different sets of array configurations for multichannel 2D ERT acquisition

NASA Astrophysics Data System (ADS)

Martorana, R.; Capizzi, P.; D'Alessandro, A.; Luzio, D.

2017-02-01

Traditional electrode arrays such Wenner-Schlumberger or dipole-dipole are still widely used thanks to their well-known properties but the array configurations are generally not optimized for multi-channel resistivity measures. Synthetic datasets relating to four different arrays, dipole-dipole (DD), pole-dipole (PD), Wenner-Schlumberger (WS) and a modified version of multiple gradient (MG), have been made for a systematic comparison between 2D resistivity models and their inverted images. Different sets of array configurations generated from simple combinations of geometric parameters (potential dipole lengths and dipole separation factors) were tested with synthetic and field data sets, even considering the influence of errors and the acquisition velocity. The purpose is to establish array configurations capable to provide reliable results but, at the same time, not involving excessive survey costs, even linked to the acquiring time and therefore to the number of current dipoles used. For DD, PD and WS arrays a progression of different datasets were considered increasing the number of current dipoles trying to get about the same amount of measures. A multi-coverage MG array configuration is proposed by increasing the lateral coverage and so the number of current dipoles. Noise simulating errors both on the electrode positions and on the electric potential was added. The array configurations have been tested on field data acquired in the landfill site of Bellolampo (Palermo, Italy), to detect and locate the leachate plumes and to identify the HDPE bottom of the landfill. The inversion results were compared using a quantitative analysis of data misfit, relative model resolution and model misfit. The results show that the trends of the first two parameters are linked on the array configuration and that a cumulative analysis of these parameters can help to choose the best array configuration in order to obtain a good resolution and reliability of a survey, according to generally short acquisition times.

A Novel Approach to Beam Steering Using Arrays Composed of Multiple Unique Radiating Modes

NASA Astrophysics Data System (ADS)

Labadie, Nathan Richard

Phased array antennas have found wide application in both radar and wireless communications systems particularly as implementation costs continue to decrease. The primary advantages of electronically scanned arrays are speed of beam scan and versatility of beamforming compared to mechanically scanned fixed beam antennas. These benefits come at the cost of a few well known design issues including element pattern rolloff and mutual coupling between elements. Our primary contribution to the field of research is the demonstration of significant improvement in phased array scan performance using multiple unique radiating modes. In short, orthogonal radiating modes have minimal coupling by definition and can also be generated with reduced rolloff at wide scan angles. In this dissertation, we present a combination of analysis, full-wave electromagnetic simulation and measured data to support our claims. The novel folded ring resonator (FRR) antenna is introduced as a wideband and multi-band element embedded in a grounded dielectric substrate. Multiple radiating modes of a small ground plane excited by a four element FRR array were also investigated. A novel hemispherical null steering antenna composed of two collocated radiating elements, each supporting a unique radiating mode, is presented in the context of an anti-jam GPS receiver application. Both the antenna aperture and active feed network were fabricated and measured showing excellent agreement with analytical and simulated data. The concept of using an antenna supporting multiple radiating modes for beam steering is also explored. A 16 element hybrid linear phased array was fabricated and measured demonstrating significantly improved scan range and scanned gain compared to a conventional phased array. This idea is expanded to 2 dimensional scanning arrays by analysis and simulation of a hybrid phased array composed of novel multiple mode monopole on patch antenna sub-arrays. Finally, we fabricated and characterized the 2D scanning hybrid phased array demonstrating wide angle scanning with high antenna efficiency.

Microarray platform for omics analysis

NASA Astrophysics Data System (ADS)

Mecklenburg, Michael; Xie, Bin

2001-09-01

Microarray technology has revolutionized genetic analysis. However, limitations in genome analysis has lead to renewed interest in establishing 'omic' strategies. As we enter the post-genomic era, new microarray technologies are needed to address these new classes of 'omic' targets, such as proteins, as well as lipids and carbohydrates. We have developed a microarray platform that combines self- assembling monolayers with the biotin-streptavidin system to provide a robust, versatile immobilization scheme. A hydrophobic film is patterned on the surface creating an array of tension wells that eliminates evaporation effects thereby reducing the shear stress to which biomolecules are exposed to during immobilization. The streptavidin linker layer makes it possible to adapt and/or develop microarray based assays using virtually any class of biomolecules including: carbohydrates, peptides, antibodies, receptors, as well as them ore traditional DNA based arrays. Our microarray technology is designed to furnish seamless compatibility across the various 'omic' platforms by providing a common blueprint for fabricating and analyzing arrays. The prototype microarray uses a microscope slide footprint patterned with 2 by 96 flat wells. Data on the microarray platform will be presented.

Disposable MoS2-Arrayed MALDI MS Chip for High-Throughput and Rapid Quantification of Sulfonamides in Multiple Real Samples.

PubMed

Zhao, Yaju; Tang, Minmin; Liao, Qiaobo; Li, Zhoumin; Li, Hui; Xi, Kai; Tan, Li; Zhang, Mei; Xu, Danke; Chen, Hong-Yuan

2018-04-27

In this work, we demonstrate, for the first time, the development of a disposable MoS 2 -arrayed matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) chip combined with an immunoaffinity enrichment method for high-throughput, rapid, and simultaneous quantitation of multiple sulfonamides (SAs). The disposable MALDI MS chip was designed and fabricated by MoS 2 array formation on a commercial indium tin oxide (ITO) glass slide. A series of SAs were analyzed, and clear deprotonated signals were obtained in negative-ion mode. Compared with MoS 2 -arrayed commercial steel plate, the prepared MALDI MS chip exhibited comparable LDI efficiency, providing a good alternative and disposable substrate for MALDI MS analysis. Furthermore, internal standard (IS) was previously deposited onto the MoS 2 array to simplify the experimental process for MALDI MS quantitation. 96 sample spots could be analyzed within 10 min in one single chip to perform quantitative analysis, recovery studies, and real foodstuff detection. Upon targeted extraction and enrichment by antibody conjugated magnetic beads, five SAs were quantitatively determined by the IS-first method with the linear range of 0.5-10 ng/mL ( R 2 > 0.990). Good recoveries and repeatability were obtained for spiked pork, egg, and milk samples. SAs in several real foodstuffs were successfully identified and quantified. The developed method may provide a promising tool for the routine analysis of antibiotic residues in real samples.

Directivity of a Sparse Array in the Presence of Atmospheric-Induced Phase Fluctuations for Deep Space Communications

NASA Technical Reports Server (NTRS)

Nessel, James A.; Acosta, Robert J.

2010-01-01

Widely distributed (sparse) ground-based arrays have been utilized for decades in the radio science community for imaging celestial objects, but have only recently become an option for deep space communications applications with the advent of the proposed Next Generation Deep Space Network (DSN) array. But whereas in astronomical imaging, observations (receive-mode only) are made on the order of minutes to hours and atmospheric-induced aberrations can be mostly corrected for in post-processing, communications applications require transmit capabilities and real-time corrections over time scales as short as fractions of a second. This presents an unavoidable problem with the use of sparse arrays for deep space communications at Ka-band which has yet to be successfully resolved, particularly for uplink arraying. In this paper, an analysis of the performance of a sparse antenna array, in terms of its directivity, is performed to derive a closed form solution to the expected array loss in the presence of atmospheric-induced phase fluctuations. The theoretical derivation for array directivity degradation is validated with interferometric measurements for a two-element array taken at Goldstone, California. With the validity of the model established, an arbitrary 27-element array geometry is defined at Goldstone, California, to ascertain its performance in the presence of phase fluctuations. It is concluded that a combination of compact array geometry and atmospheric compensation is necessary to ensure high levels of availability.

Hot spot dynamics in carbon nanotube array devices.

PubMed

Engel, Michael; Steiner, Mathias; Seo, Jung-Woo T; Hersam, Mark C; Avouris, Phaedon

2015-03-11

We report on the dynamics of spatial temperature distributions in aligned semiconducting carbon nanotube array devices with submicrometer channel lengths. By using high-resolution optical microscopy in combination with electrical transport measurements, we observe under steady state bias conditions the emergence of time-variable, local temperature maxima with dimensions below 300 nm, and temperatures above 400 K. On the basis of time domain cross-correlation analysis, we investigate how the intensity fluctuations of the thermal radiation patterns are correlated with the overall device current. The analysis reveals the interdependence of electrical current fluctuations and time-variable hot spot formation that limits the overall device performance and, ultimately, may cause device degradation. The findings have implications for the future development of carbon nanotube-based technologies.

Pupil geometry and pupil re-imaging in telescope arrays

NASA Technical Reports Server (NTRS)

Traub, Wesley A.

1990-01-01

This paper considers the issues of lateral and longitudinal pupil geometry in ground-based telescope arrays, such as IOTA. In particular, it is considered whether or not pupil re-imaging is required before beam combination. By considering the paths of rays through the system, an expression is derived for the optical path errors in the combined wavefront as a function of array dimensions, telescope magnification factor, viewing angle, and field-of-view. By examining this expression for the two cases of pupil-plane and image-plane combination, operational limits can be found for any array. As a particular example, it is shown that for IOTA no pupil re-imaging optics will be needed.

Performance degradation mechanisms and modes in terrestrial photovoltaic arrays and technology for their diagnosis

NASA Technical Reports Server (NTRS)

Noel, G. T.; Sliemers, F. A.; Derringer, G. C.; Wood, V. E.; Wilkes, K. E.; Gaines, G. B.; Carmichael, D. C.

1978-01-01

Accelerated life-prediction test methodologies have been developed for the validation of a 20-year service life for low-cost photovoltaic arrays. Array failure modes, relevant materials property changes, and primary degradation mechanisms are discussed as a prerequisite to identifying suitable measurement techniques and instruments. Measurements must provide sufficient confidence to permit selection among alternative designs and materials and to stimulate widespread deployment of such arrays. Furthermore, the diversity of candidate materials and designs, and the variety of potential environmental stress combinations, degradation mechanisms and failure modes require that combinations of measurement techniques be identified which are suitable for the characterization of various encapsulation system-cell structure-environment combinations.

Analysis of light emitting diode array lighting system based on human vision: normal and abnormal uniformity condition.

PubMed

Qin, Zong; Ji, Chuangang; Wang, Kai; Liu, Sheng

2012-10-08

In this paper, condition for uniform lighting generated by light emitting diode (LED) array was systematically studied. To take human vision effect into consideration, contrast sensitivity function (CSF) was novelly adopted as critical criterion for uniform lighting instead of conventionally used Sparrow's Criterion (SC). Through CSF method, design parameters including system thickness, LED pitch, LED's spatial radiation distribution and viewing condition can be analytically combined. In a specific LED array lighting system (LALS) with foursquare LED arrangement, different types of LEDs (Lambertian and Batwing type) and given viewing condition, optimum system thicknesses and LED pitches were calculated and compared with those got through SC method. Results show that CSF method can achieve more appropriate optimum parameters than SC method. Additionally, an abnormal phenomenon that uniformity varies with structural parameters non-monotonically in LALS with non-Lambertian LEDs was found and analyzed. Based on the analysis, a design method of LALS that can bring about better practicability, lower cost and more attractive appearance was summarized.

Quaternized magnetic nanoparticles-fluorescent polymer system for detection and identification of bacteria.

PubMed

Wan, Yi; Sun, Yan; Qi, Peng; Wang, Peng; Zhang, Dun

2014-05-15

Nanomaterial-based 'chemical nose' sensor with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. A sensor array containing three quaternized magnetic nanoparticles (q-MNPs)-fluorescent polymer systems has been designed to identify and quantify bacteria. The bacterial cell membranes disrupt the q-MNP-fluorescent polymer, generating unique fluorescence response array. The response intensity of the array is dependent on the level of displacement determined by the relative q-MNP-fluorescent polymer binding strength and bacteria cells-MNP interaction. These characteristic responses show a highly repeatable bacteria cells and can be differentiated by linear discriminant analysis (LDA). Based on the array response matrix from LDA, our approach has been used to measure bacteria with an accuracy of 87.5% for 10(7) cfu mL(-1) within 20 min. Combined with UV-vis measurement, the method can be successfully performed to identify and detect eight different pathogen samples with an accuracy of 96.8%. The measurement system has a potential for further applications and provides a facile and simple method for the rapid analysis of protein, DNA, and pathogens. Copyright © 2013 Elsevier B.V. All rights reserved.

Scattering and radiation analysis of three-dimensional cavity arrays via a hybrid finite element method

NASA Technical Reports Server (NTRS)

Jin, Jian-Ming; Volakis, John L.

1992-01-01

A hybrid numerical technique is presented for a characterization of the scattering and radiation properties of three-dimensional cavity arrays recessed in a ground plane. The technique combines the finite element and boundary integral methods and invokes Floquet's representation to formulate a system of equations for the fields at the apertures and those inside the cavities. The system is solved via the conjugate gradient method in conjunction with the Fast Fourier Transform (FFT) thus achieving an O(N) storage requirement. By virtue of the finite element method, the proposed technique is applicable to periodic arrays comprised of cavities having arbitrary shape and filled with inhomogeneous dielectrics. Several numerical results are presented, along with new measured data, which demonstrate the validity, efficiency, and capability of the technique.

ATMAD: robust image analysis for Automatic Tissue MicroArray De-arraying.

PubMed

Nguyen, Hoai Nam; Paveau, Vincent; Cauchois, Cyril; Kervrann, Charles

2018-04-19

Over the last two decades, an innovative technology called Tissue Microarray (TMA), which combines multi-tissue and DNA microarray concepts, has been widely used in the field of histology. It consists of a collection of several (up to 1000 or more) tissue samples that are assembled onto a single support - typically a glass slide - according to a design grid (array) layout, in order to allow multiplex analysis by treating numerous samples under identical and standardized conditions. However, during the TMA manufacturing process, the sample positions can be highly distorted from the design grid due to the imprecision when assembling tissue samples and the deformation of the embedding waxes. Consequently, these distortions may lead to severe errors of (histological) assay results when the sample identities are mismatched between the design and its manufactured output. The development of a robust method for de-arraying TMA, which localizes and matches TMA samples with their design grid, is therefore crucial to overcome the bottleneck of this prominent technology. In this paper, we propose an Automatic, fast and robust TMA De-arraying (ATMAD) approach dedicated to images acquired with brightfield and fluorescence microscopes (or scanners). First, tissue samples are localized in the large image by applying a locally adaptive thresholding on the isotropic wavelet transform of the input TMA image. To reduce false detections, a parametric shape model is considered for segmenting ellipse-shaped objects at each detected position. Segmented objects that do not meet the size and the roundness criteria are discarded from the list of tissue samples before being matched with the design grid. Sample matching is performed by estimating the TMA grid deformation under the thin-plate model. Finally, thanks to the estimated deformation, the true tissue samples that were preliminary rejected in the early image processing step are recognized by running a second segmentation step. We developed a novel de-arraying approach for TMA analysis. By combining wavelet-based detection, active contour segmentation, and thin-plate spline interpolation, our approach is able to handle TMA images with high dynamic, poor signal-to-noise ratio, complex background and non-linear deformation of TMA grid. In addition, the deformation estimation produces quantitative information to asset the manufacturing quality of TMAs.

Residual and suppressed-carrier arraying techniques for deep-space communications

NASA Technical Reports Server (NTRS)

Shihabi, M.; Shah, B.; Hinedi, S.; Million, S.

1995-01-01

Three techniques that use carrier information from multiple antennas to enhance carrier acquisition and tracking are presented. These techniques in combination with baseband combining are analyzed and simulated for residual and suppressed-carrier modulation. It is shown that the carrier arraying using a single carrier loop technique can acquire and track the carrier even when any single antenna in the array cannot do so by itself. The carrier aiding and carrier arraying using multiple carrier loop techniques, on the other hand, are shown to lock on the carrier only when one of the array elements has sufficient margin to acquire the carrier on its own.

Characterization of physalins and fingerprint analysis for the quality evaluation of Physalis alkekengi L. var. franchetii by ultra-performance liquid chromatography combined with diode array detection and electrospray ionization tandem mass spectrometry.

PubMed

Zheng, Yunliang; Luan, Lianjun; Chen, Yong; Ren, Yiping; Wu, Yongjiang

2012-12-01

Physalins are important bioactive compounds from genus Physalis. They often occur as isomers, which makes the structural elucidation difficult. In the present study, the fragmentation behavior and UV characteristics of seven physalins from genus Physalis were firstly investigated using electrospray ionization tandem mass spectrometry (ESI-MS/MS) and diode array detection (DAD). Combined with ultra-performance liquid chromatography (UPLC) and DAD, the established approach to the structural identification of physalins by ESI-MS/MS was then applied to the analysis of Physalis alkekengi L. According to the UPLC retention behavior, the diagnostic UV spectra and the molecular structural information provided by MS/MS spectra, about 19 fingerprint peaks were identified, including 14 physalins and 5 other compounds. Finally, the established fingerprint method was applied to the analysis of 31 P. alkekengi L. samples collected from different locations, which reflected their similar chemical constituent properties. The proposed method provides a scientific and technical platform to the herbal industry for quality control and safety assurance of herbal preparations that contain this class of physalins. Copyright © 2012 Elsevier B.V. All rights reserved.

Linear Combination of Heuristics Approach to Spatial Sampling Hyperspectral Data for Target Tracking

DTIC Science & Technology

2010-12-01

Figure 37 - Illustration of the tunable spectral polarimeter. ........................................... 154 Figure 38 - Illustration of micromirrors ...polarimeter. 9.2 Multiobject Tracking Spectrometer The idea of combining an array of MEMS micromirrors with an imager and a spectrometer array is the... micromirror array is located at an intermediate focal plane of the optical system. If all the individual mirrors are turned in the same direction

The revised solar array synthesis computer program

NASA Technical Reports Server (NTRS)

1970-01-01

The Revised Solar Array Synthesis Computer Program is described. It is a general-purpose program which computes solar array output characteristics while accounting for the effects of temperature, incidence angle, charged-particle irradiation, and other degradation effects on various solar array configurations in either circular or elliptical orbits. Array configurations may consist of up to 75 solar cell panels arranged in any series-parallel combination not exceeding three series-connected panels in a parallel string and no more than 25 parallel strings in an array. Up to 100 separate solar array current-voltage characteristics, corresponding to 100 equal-time increments during the sunlight illuminated portion of an orbit or any 100 user-specified combinations of incidence angle and temperature, can be computed and printed out during one complete computer execution. Individual panel incidence angles may be computed and printed out at the user's option.

Chemistry integrated circuit: chemical system on a complementary metal oxide semiconductor integrated circuit.

PubMed

Nakazato, Kazuo

2014-03-28

By integrating chemical reactions on a large-scale integration (LSI) chip, new types of device can be created. For biomedical applications, monolithically integrated sensor arrays for potentiometric, amperometric and impedimetric sensing of biomolecules have been developed. The potentiometric sensor array detects pH and redox reaction as a statistical distribution of fluctuations in time and space. For the amperometric sensor array, a microelectrode structure for measuring multiple currents at high speed has been proposed. The impedimetric sensor array is designed to measure impedance up to 10 MHz. The multimodal sensor array will enable synthetic analysis and make it possible to standardize biosensor chips. Another approach is to create new functional devices by integrating molecular systems with LSI chips, for example image sensors that incorporate biological materials with a sensor array. The quantum yield of the photoelectric conversion of photosynthesis is 100%, which is extremely difficult to achieve by artificial means. In a recently developed process, a molecular wire is plugged directly into a biological photosynthetic system to efficiently conduct electrons to a gold electrode. A single photon can be detected at room temperature using such a system combined with a molecular single-electron transistor.

Comparative efficiency analysis of fiber-array and conventional beam director systems in volume turbulence.

PubMed

Vorontsov, Mikhail; Filimonov, Grigory; Ovchinnikov, Vladimir; Polnau, Ernst; Lachinova, Svetlana; Weyrauch, Thomas; Mangano, Joseph

2016-05-20

The performance of two prominent laser beam projection system types is analyzed through wave-optics numerical simulations for various atmospheric turbulence conditions, propagation distances, and adaptive optics (AO) mitigation techniques. Comparisons are made between different configurations of both a conventional beam director (BD) using a monolithic-optics-based Cassegrain telescope and a fiber-array BD that uses an array of densely packed fiber collimators. The BD systems considered have equal input power and aperture diameters. The projected laser beam power inside the Airy size disk at the target plane is used as the performance metric. For the fiber-array system, both incoherent and coherent beam combining regimes are considered. We also present preliminary results of side-by-side atmospheric beam projection experiments over a 7-km propagation path using both the AO-enhanced beam projection system with a Cassegrain telescope and the coherent fiber-array BD composed of 21 densely packed fiber collimators. Both wave-optics numerical simulation and experimental results demonstrate that, for similar system architectures and turbulence conditions, coherent fiber-array systems are more efficient in mitigation of atmospheric turbulence effects and generation of a hit spot of the smallest possible size on a remotely located target.

A Streamlined Protocol for Molecular Testing of the DMD Gene within a Diagnostic Laboratory: A Combination of Array Comparative Genomic Hybridization and Bidirectional Sequence Analysis

PubMed Central

Marquis-Nicholson, Renate; Lai, Daniel; Love, Jennifer M.; Love, Donald R.

2013-01-01

Purpose. The aim of this study was to develop a streamlined mutation screening protocol for the DMD gene in order to confirm a clinical diagnosis of Duchenne or Becker muscular dystrophy in affected males and to clarify the carrier status of female family members. Methods. Sequence analysis and array comparative genomic hybridization (aCGH) were used to identify mutations in the dystrophin DMD gene. We analysed genomic DNA from six individuals with a range of previously characterised mutations and from eight individuals who had not previously undergone any form of molecular analysis. Results. We successfully identified the known mutations in all six patients. A molecular diagnosis was also made in three of the four patients with a clinical diagnosis who had not undergone prior genetic screening, and testing for familial mutations was successfully completed for the remaining four patients. Conclusion. The mutation screening protocol described here meets best practice guidelines for molecular testing of the DMD gene in a diagnostic laboratory. The aCGH method is a superior alternative to more conventional assays such as multiplex ligation-dependent probe amplification (MLPA). The combination of aCGH and sequence analysis will detect mutations in 98% of patients with the Duchenne or Becker muscular dystrophy. PMID:23476807

Improved Constraints on Cosmology and Foregrounds from BICEP2 and Keck Array Cosmic Microwave Background Data with Inclusion of 95 GHz Band.

PubMed

Ade, P A R; Ahmed, Z; Aikin, R W; Alexander, K D; Barkats, D; Benton, S J; Bischoff, C A; Bock, J J; Bowens-Rubin, R; Brevik, J A; Buder, I; Bullock, E; Buza, V; Connors, J; Crill, B P; Duband, L; Dvorkin, C; Filippini, J P; Fliescher, S; Grayson, J; Halpern, M; Harrison, S; Hilton, G C; Hui, H; Irwin, K D; Karkare, K S; Karpel, E; Kaufman, J P; Keating, B G; Kefeli, S; Kernasovskiy, S A; Kovac, J M; Kuo, C L; Leitch, E M; Lueker, M; Megerian, K G; Netterfield, C B; Nguyen, H T; O'Brient, R; Ogburn, R W; Orlando, A; Pryke, C; Richter, S; Schwarz, R; Sheehy, C D; Staniszewski, Z K; Steinbach, B; Sudiwala, R V; Teply, G P; Thompson, K L; Tolan, J E; Tucker, C; Turner, A D; Vieregg, A G; Weber, A C; Wiebe, D V; Willmert, J; Wong, C L; Wu, W L K; Yoon, K W

2016-01-22

We present results from an analysis of all data taken by the BICEP2 and Keck Array cosmic microwave background (CMB) polarization experiments up to and including the 2014 observing season. This includes the first Keck Array observations at 95 GHz. The maps reach a depth of 50 nK deg in Stokes Q and U in the 150 GHz band and 127 nK deg in the 95 GHz band. We take auto- and cross-spectra between these maps and publicly available maps from WMAP and Planck at frequencies from 23 to 353 GHz. An excess over lensed ΛCDM is detected at modest significance in the 95×150 BB spectrum, and is consistent with the dust contribution expected from our previous work. No significant evidence for synchrotron emission is found in spectra such as 23×95, or for correlation between the dust and synchrotron sky patterns in spectra such as 23×353. We take the likelihood of all the spectra for a multicomponent model including lensed ΛCDM, dust, synchrotron, and a possible contribution from inflationary gravitational waves (as parametrized by the tensor-to-scalar ratio r) using priors on the frequency spectral behaviors of dust and synchrotron emission from previous analyses of WMAP and Planck data in other regions of the sky. This analysis yields an upper limit r_{0.05}<0.09 at 95% confidence, which is robust to variations explored in analysis and priors. Combining these B-mode results with the (more model-dependent) constraints from Planck analysis of CMB temperature plus baryon acoustic oscillations and other data yields a combined limit r_{0.05}<0.07 at 95% confidence. These are the strongest constraints to date on inflationary gravitational waves.

Joint analysis of BICEP2/keck array and Planck Data.

PubMed

Ade, P A R; Aghanim, N; Ahmed, Z; Aikin, R W; Alexander, K D; Arnaud, M; Aumont, J; Baccigalupi, C; Banday, A J; Barkats, D; Barreiro, R B; Bartlett, J G; Bartolo, N; Battaner, E; Benabed, K; Benoît, A; Benoit-Lévy, A; Benton, S J; Bernard, J-P; Bersanelli, M; Bielewicz, P; Bischoff, C A; Bock, J J; Bonaldi, A; Bonavera, L; Bond, J R; Borrill, J; Bouchet, F R; Boulanger, F; Brevik, J A; Bucher, M; Buder, I; Bullock, E; Burigana, C; Butler, R C; Buza, V; Calabrese, E; Cardoso, J-F; Catalano, A; Challinor, A; Chary, R-R; Chiang, H C; Christensen, P R; Colombo, L P L; Combet, C; Connors, J; Couchot, F; Coulais, A; Crill, B P; Curto, A; Cuttaia, F; Danese, L; Davies, R D; Davis, R J; de Bernardis, P; de Rosa, A; de Zotti, G; Delabrouille, J; Delouis, J-M; Désert, F-X; Dickinson, C; Diego, J M; Dole, H; Donzelli, S; Doré, O; Douspis, M; Dowell, C D; Duband, L; Ducout, A; Dunkley, J; Dupac, X; Dvorkin, C; Efstathiou, G; Elsner, F; Enßlin, T A; Eriksen, H K; Falgarone, E; Filippini, J P; Finelli, F; Fliescher, S; Forni, O; Frailis, M; Fraisse, A A; Franceschi, E; Frejsel, A; Galeotta, S; Galli, S; Ganga, K; Ghosh, T; Giard, M; Gjerløw, E; Golwala, S R; González-Nuevo, J; Górski, K M; Gratton, S; Gregorio, A; Gruppuso, A; Gudmundsson, J E; Halpern, M; Hansen, F K; Hanson, D; Harrison, D L; Hasselfield, M; Helou, G; Henrot-Versillé, S; Herranz, D; Hildebrandt, S R; Hilton, G C; Hivon, E; Hobson, M; Holmes, W A; Hovest, W; Hristov, V V; Huffenberger, K M; Hui, H; Hurier, G; Irwin, K D; Jaffe, A H; Jaffe, T R; Jewell, J; Jones, W C; Juvela, M; Karakci, A; Karkare, K S; Kaufman, J P; Keating, B G; Kefeli, S; Keihänen, E; Kernasovskiy, S A; Keskitalo, R; Kisner, T S; Kneissl, R; Knoche, J; Knox, L; Kovac, J M; Krachmalnicoff, N; Kunz, M; Kuo, C L; Kurki-Suonio, H; Lagache, G; Lähteenmäki, A; Lamarre, J-M; Lasenby, A; Lattanzi, M; Lawrence, C R; Leitch, E M; Leonardi, R; Levrier, F; Lewis, A; Liguori, M; Lilje, P B; Linden-Vørnle, M; López-Caniego, M; Lubin, P M; Lueker, M; Macías-Pérez, J F; Maffei, B; Maino, D; Mandolesi, N; Mangilli, A; Maris, M; Martin, P G; Martínez-González, E; Masi, S; Mason, P; Matarrese, S; Megerian, K G; Meinhold, P R; Melchiorri, A; Mendes, L; Mennella, A; Migliaccio, M; Mitra, S; Miville-Deschênes, M-A; Moneti, A; Montier, L; Morgante, G; Mortlock, D; Moss, A; Munshi, D; Murphy, J A; Naselsky, P; Nati, F; Natoli, P; Netterfield, C B; Nguyen, H T; Nørgaard-Nielsen, H U; Noviello, F; Novikov, D; Novikov, I; O'Brient, R; Ogburn, R W; Orlando, A; Pagano, L; Pajot, F; Paladini, R; Paoletti, D; Partridge, B; Pasian, F; Patanchon, G; Pearson, T J; Perdereau, O; Perotto, L; Pettorino, V; Piacentini, F; Piat, M; Pietrobon, D; Plaszczynski, S; Pointecouteau, E; Polenta, G; Ponthieu, N; Pratt, G W; Prunet, S; Pryke, C; Puget, J-L; Rachen, J P; Reach, W T; Rebolo, R; Reinecke, M; Remazeilles, M; Renault, C; Renzi, A; Richter, S; Ristorcelli, I; Rocha, G; Rossetti, M; Roudier, G; Rowan-Robinson, M; Rubiño-Martín, J A; Rusholme, B; Sandri, M; Santos, D; Savelainen, M; Savini, G; Schwarz, R; Scott, D; Seiffert, M D; Sheehy, C D; Spencer, L D; Staniszewski, Z K; Stolyarov, V; Sudiwala, R; Sunyaev, R; Sutton, D; Suur-Uski, A-S; Sygnet, J-F; Tauber, J A; Teply, G P; Terenzi, L; Thompson, K L; Toffolatti, L; Tolan, J E; Tomasi, M; Tristram, M; Tucci, M; Turner, A D; Valenziano, L; Valiviita, J; Van Tent, B; Vibert, L; Vielva, P; Vieregg, A G; Villa, F; Wade, L A; Wandelt, B D; Watson, R; Weber, A C; Wehus, I K; White, M; White, S D M; Willmert, J; Wong, C L; Yoon, K W; Yvon, D; Zacchei, A; Zonca, A

2015-03-13

We report the results of a joint analysis of data from BICEP2/Keck Array and Planck. BICEP2 and Keck Array have observed the same approximately 400  deg^{2} patch of sky centered on RA 0 h, Dec. -57.5°. The combined maps reach a depth of 57 nK deg in Stokes Q and U in a band centered at 150 GHz. Planck has observed the full sky in polarization at seven frequencies from 30 to 353 GHz, but much less deeply in any given region (1.2  μK deg in Q and U at 143 GHz). We detect 150×353 cross-correlation in B modes at high significance. We fit the single- and cross-frequency power spectra at frequencies ≥150  GHz to a lensed-ΛCDM model that includes dust and a possible contribution from inflationary gravitational waves (as parametrized by the tensor-to-scalar ratio r), using a prior on the frequency spectral behavior of polarized dust emission from previous Planck analysis of other regions of the sky. We find strong evidence for dust and no statistically significant evidence for tensor modes. We probe various model variations and extensions, including adding a synchrotron component in combination with lower frequency data, and find that these make little difference to the r constraint. Finally, we present an alternative analysis which is similar to a map-based cleaning of the dust contribution, and show that this gives similar constraints. The final result is expressed as a likelihood curve for r, and yields an upper limit r_{0.05}<0.12 at 95% confidence. Marginalizing over dust and r, lensing B modes are detected at 7.0σ significance.

Glossary

MedlinePlus

... array, and oligo/SNP combination array. Related terms: comparative genomic hybridization ; copy number variant ; SNP array chromosome ... for example, the AB blood groups in humans comparative genomic hybridization Method in which two DNA samples ( ...

Ethical hot spots of combined individual and group therapy: applying four ethical systems.

PubMed

Brabender, Virginia M; Fallon, April

2009-01-01

Abstract Combined therapy presents ethical quandaries that occur in individual psychotherapy and group psychotherapy, and dilemmas specifically associated with their integration. This paper examines two types of ethical frameworks (a classical principle-based framework and a set of context-based frameworks) for addressing the ethical hot spots of combined therapy: self-referral, transfer of information, and termination. The principle-based approach enables the practitioner to see what core values may be served or violated by different courses of action in combined therapy dilemmas. Yet, the therapist is more likely to do justice to the complexity and richness of the combined therapy situation by supplementing a principle analysis with three additional ethical frameworks. These approaches are: virtue ethics, feminist ethics, and casuistry. An analysis of three vignettes illustrates how these contrasting ethical models not only expand the range of features to which the therapist attends but also the array of solutions the therapist generates.

Infrared imaging of cotton fiber bundles using a focal plane array detector and a single reflectance accessory

USDA-ARS?s Scientific Manuscript database

Infrared imaging is gaining attention as a technique used in the examination of cotton fibers. This type of imaging combines spectral analysis with spatial resolution to create visual images that examine sample composition and distribution. Herein, we report the use of an infrared instrument equippe...

Generalization and fine mapping of European ancestry-based central adiposity variants in African ancestry populations

PubMed Central

Yoneyama, Sachiko; Yao, Jie; Guo, Xiuqing; Fernandez-Rhodes, Lindsay; Lim, Unhee; Boston, Jonathan; Buzková, Petra; Carlson, Christopher S.; Cheng, Iona; Cochran, Barbara; Cooper, Richard; Ehret, Georg; Fornage, Myriam; Gong, Jian; Gross, Myron; Gu, C. Charles; Haessler, Jeff; Haiman, Christopher A.; Henderson, Brian; Hindorff, Lucia A.; Houston, Denise; Irvin, Marguerite R.; Jackson, Rebecca; Kuller, Lew; Leppert, Mark; Lewis, Cora E.; Li, Rongling; Le Marchand, Loic; Matise, Tara C.; Nguyen, Khanh-Dung H.; Chakravarti, Aravinda; Pankow, James S.; Pankratz, Nathan; Pooler, Loreall; Ritchie, Marylyn D.; Bien, Stephanie A.; Wassel, Christina L.; Chen, Yii-Der I.; Taylor, Kent D.; Allison, Matthew; Rotter, Jerome I.; Schreiner, Pamela J.; Schumacher, Fredrick; Wilkens, Lynne; Boerwinkle, Eric; Kooperberg, Charles; Peters, Ulrike; Buyske, Steven; Graff, Mariaelisa; North, Kari E.

2016-01-01

Background/Objectives Central adiposity measures such as waist circumference (WC) and waist-to-hip ratio (WHR) are associated with cardiometabolic disorders independently of BMI and are gaining clinically utility. Several studies report genetic variants associated with central adiposity, but most utilize only European ancestry populations. Understanding whether the genetic associations discovered among mainly European descendants are shared with African ancestry populations will help elucidate the biological underpinnings of abdominal fat deposition. Subjects/Methods To identify the underlying functional genetic determinants of body fat distribution, we conducted an array-wide association meta-analysis among persons of African ancestry across seven studies/consortia participating in the Population Architecture using Genomics and Epidemiology (PAGE) consortium. We used the Metabochip array, designed for fine mapping cardiovascular associated loci, to explore novel array-wide associations with WC and WHR among 15 945 African descendants using all and sex-stratified groups. We further interrogated 17 known WHR regions for African ancestry-specific variants. Results Of the 17 WHR loci, eight SNPs located in four loci were replicated in the sex-combined or sex-stratified meta-analyses. Two of these eight independently associated with WHR after conditioning on the known variant in European descendants (rs12096179 in TBX15-WARS2 and rs2059092 in ADAMTS9). In the fine mapping assessment, the putative functional region was reduced across all four loci but to varying degrees (average 40% drop in number of putative SNPs and 20% drop in genomic region). Similar to previous studies, the significant SNPs in the female stratified analysis were stronger than the significant SNPs from the sex-combined analysis. No novel associations were detected in the array-wide analyses. Conclusions Of 17 previously identified loci, four loci replicated in the African ancestry populations of this study. Utilizing different linkage disequilibrium patterns observed between European and African ancestries, we narrowed the suggestive region containing causative variants for all four loci. PMID:27867202

Combined micro-Raman/UV-visible/fluorescence spectrometer for high-throughput analysis of microsamples.

PubMed

Noh, Jermim; Suh, Yung Doug; Park, Yong Ki; Jin, Seung Min; Kim, Soo Ho; Woo, Seong Ihl

2007-07-01

Combined micro-Raman/UV-visible (vis)/fluorescence spectroscopy system, which can evaluate an integrated array of more than 10,000 microsamples with a minimuma size of 5 microm within a few hours, has been developed for the first time. The array of microsamples is positioned on a computer-controlled XY translation microstage with a spatial resolution of 1 mum so that the spectra can be mapped with micron precision. Micro-Raman spectrometers have a high spectral resolution of about 2 cm(-1) over the wave number range of 150-3900 cm(-1), while UV-vis and fluorescence spectrometers have high spectral resolutions of 0.4 and 0.1 nm over the wavelength range of 190-900 nm, respectively. In particular, the signal-to-noise ratio of the micro-Raman spectroscopy has been improved by using a holographic Raman grating and a liquid-nitrogen-cooled charge-coupled device detector. The performance of the combined spectroscopy system has been demonstrated by the high-throughput screening of a combinatorial ferroelectric (i.e., BaTi(x)Zr(1-x)O(3)) library. This system makes possible the structure analysis of various materials including ferroelectrics, catalysts, phosphors, polymers, alloys, and so on for the development of novel materials and the ultrasensitive detection of trace amounts of pharmaceuticals and diagnostic agents.

Calibration artefacts in radio interferometry - II. Ghost patterns for irregular arrays

NASA Astrophysics Data System (ADS)

Wijnholds, S. J.; Grobler, T. L.; Smirnov, O. M.

2016-04-01

Calibration artefacts, like the self-calibration bias, usually emerge when data are calibrated using an incomplete sky model. In the first paper of this series, in which we analysed calibration artefacts in data from the Westerbork Synthesis Radio Telescope, we showed that these artefacts take the form of spurious positive and negative sources, which we refer to as ghosts or ghost sources. We also developed a mathematical framework with which we could predict the ghost pattern of an east-west interferometer for a simple two-source test case. In this paper, we extend our analysis to more general array layouts. This provides us with a useful method for the analysis of ghosts that we refer to as extrapolation. Combining extrapolation with a perturbation analysis, we are able to (1) analyse the ghost pattern for a two-source test case with one modelled and one unmodelled source for an arbitrary array layout, (2) explain why some ghosts are brighter than others, (3) define a taxonomy allowing us to classify the different ghosts, (4) derive closed form expressions for the fluxes and positions of the brightest ghosts, and (5) explain the strange two-peak structure with which some ghosts manifest during imaging. We illustrate our mathematical predictions using simulations of the KAT-7 (seven-dish Karoo Array Telescope) array. These results show the explanatory power of our mathematical model. The insights gained in this paper provide a solid foundation to study calibration artefacts in arbitrary, I.e. more complicated than the two-source example discussed here, incomplete sky models or full synthesis observations including direction-dependent effects.

High-throughput fabrication and screening improves gold nanoparticle chemiresistor sensor performance.

PubMed

Hubble, Lee J; Cooper, James S; Sosa-Pintos, Andrea; Kiiveri, Harri; Chow, Edith; Webster, Melissa S; Wieczorek, Lech; Raguse, Burkhard

2015-02-09

Chemiresistor sensor arrays are a promising technology to replace current laboratory-based analysis instrumentation, with the advantage of facile integration into portable, low-cost devices for in-field use. To increase the performance of chemiresistor sensor arrays a high-throughput fabrication and screening methodology was developed to assess different organothiol-functionalized gold nanoparticle chemiresistors. This high-throughput fabrication and testing methodology was implemented to screen a library consisting of 132 different organothiol compounds as capping agents for functionalized gold nanoparticle chemiresistor sensors. The methodology utilized an automated liquid handling workstation for the in situ functionalization of gold nanoparticle films and subsequent automated analyte testing of sensor arrays using a flow-injection analysis system. To test the methodology we focused on the discrimination and quantitation of benzene, toluene, ethylbenzene, p-xylene, and naphthalene (BTEXN) mixtures in water at low microgram per liter concentration levels. The high-throughput methodology identified a sensor array configuration consisting of a subset of organothiol-functionalized chemiresistors which in combination with random forests analysis was able to predict individual analyte concentrations with overall root-mean-square errors ranging between 8-17 μg/L for mixtures of BTEXN in water at the 100 μg/L concentration. The ability to use a simple sensor array system to quantitate BTEXN mixtures in water at the low μg/L concentration range has direct and significant implications to future environmental monitoring and reporting strategies. In addition, these results demonstrate the advantages of high-throughput screening to improve the performance of gold nanoparticle based chemiresistors for both new and existing applications.

A Systolic Array-Based FPGA Parallel Architecture for the BLAST Algorithm

PubMed Central

Guo, Xinyu; Wang, Hong; Devabhaktuni, Vijay

2012-01-01

A design of systolic array-based Field Programmable Gate Array (FPGA) parallel architecture for Basic Local Alignment Search Tool (BLAST) Algorithm is proposed. BLAST is a heuristic biological sequence alignment algorithm which has been used by bioinformatics experts. In contrast to other designs that detect at most one hit in one-clock-cycle, our design applies a Multiple Hits Detection Module which is a pipelining systolic array to search multiple hits in a single-clock-cycle. Further, we designed a Hits Combination Block which combines overlapping hits from systolic array into one hit. These implementations completed the first and second step of BLAST architecture and achieved significant speedup comparing with previously published architectures. PMID:25969747

Investigation of effects of process parameters on properties of friction stir welded joints

NASA Astrophysics Data System (ADS)

Chauhan, Atul; Soota, Tarun; Rajput, S. K.

2018-03-01

This work deals with application of friction stir welding (FSW) using application of Taguchi orthogonal array. FSW procedure is used for joining the aluminium alloy AA6063-T0 plates in butt configuration with orthogonal combination of factors and their levels. The combination of factors involving tool rotation speed, tool travel speed and tool pin profile are used in three levels. Grey relational analysis (GRA) has been applied to select optimum level of factors for optimising UTS, ductility and hardness of joint. Experiments have been conducted with two different tool materials (HSS and HCHCr steel) with various factors level combinations for joining AA6063-T0. On the basis of grey relational grades at different levels of factors and analysis of variance (ANOVA) ideal combination of factors are determined. The influence of tool material is also studied.

Structures and Dynamics Division research and technology plans, FY 1982

NASA Technical Reports Server (NTRS)

Bales, K. S.

1982-01-01

Computational devices to improve efficiency for structural calculations are assessed. The potential of large arrays of microprocessors operating in parallel for finite element analysis is defined, and the impact of specialized computer hardware on static, dynamic, thermal analysis in the optimization of structural analysis and design calculations is determined. General aviation aircraft crashworthiness and occupant survivability is also considered. Mechanics technology required for design coefficient, fault tolerant advanced composite aircraft components subject to combined loads, impact, postbuckling effects and local discontinuities are developed.

Nanohole Array-directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis

PubMed Central

Kumar, Shailabh; Wolken, Gregory G.; Wittenberg, Nathan J.; Arriaga, Edgar A.; Oh, Sang-Hyun

2016-01-01

We present periodic nanohole arrays fabricated in free-standing metal-coated nitride films as a platform for trapping and analyzing single organelles. When a microliter-scale droplet containing mitochondria is dispensed above the nanohole array, the combination of evaporation and capillary flow directs individual mitochondria to the nanoholes. Mammalian mitochondria arrays were rapidly formed on chip using this technique without any surface modification steps, microfluidic interconnects or external power sources. The trapped mitochondria were depolarized on chip using an ionophore with results showing that the organelle viability and behavior were preserved during the on-chip assembly process. Fluorescence signal related to mitochondrial membrane potential was obtained from single mitochondria trapped in individual nanoholes revealing statistical differences between the behavior of polarized vs. depolarized mammalian mitochondria. This technique provides a fast and stable route for droplet-based directed localization of organelles-on-a-chip with minimal limitations and complexity, as well as promotes integration with other optical or electrochemical detection techniques. PMID:26593329

High-efficient light absorption of monolayer graphene via cylindrical dielectric arrays and the sensing application

NASA Astrophysics Data System (ADS)

Zhou, Peng; Zheng, Gaige

2018-04-01

The efficiency of graphene-based optoelectronic devices is typically limited by the poor absolute absorption of light. A hybrid structure of monolayer graphene with cylindrical titanium dioxide (TiO2) array and aluminum oxide (Al2O3) spacer layer on aluminum (Al) substrate has been proposed to enhance the absorption for two-dimensional (2D) materials. By combining dielectric array with metal substrate, the structure achieves multiple absorption peaks with near unity absorbance at near-infrared wavelengths due to the resonant effect of dielectric array. Completed monolayer graphene is utilized in the design without any demand of manufacture process to form the periodic patterns. Further analysis indicates that the near-field enhancement induced by surface modes gives rise to the high absorption. This favorable field enhancement and tunability of absorption not only open up new approaches to accelerate the light-graphene interaction, but also show great potential for practical applications in high-performance optoelectronic devices, such as modulators and sensors.

Digital processing of array seismic recordings

USGS Publications Warehouse

Ryall, Alan; Birtill, John

1962-01-01

This technical letter contains a brief review of the operations which are involved in digital processing of array seismic recordings by the methods of velocity filtering, summation, cross-multiplication and integration, and by combinations of these operations (the "UK Method" and multiple correlation). Examples are presented of analyses by the several techniques on array recordings which were obtained by the U.S. Geological Survey during chemical and nuclear explosions in the western United States. Seismograms are synthesized using actual noise and Pn-signal recordings, such that the signal-to-noise ratio, onset time and velocity of the signal are predetermined for the synthetic record. These records are then analyzed by summation, cross-multiplication, multiple correlation and the UK technique, and the results are compared. For all of the examples presented, analysis by the non-linear techniques of multiple correlation and cross-multiplication of the traces on an array recording are preferred to analyses by the linear operations involved in summation and the UK Method.

Reliability apportionment approach for spacecraft solar array using fuzzy reasoning Petri net and fuzzy comprehensive evaluation

NASA Astrophysics Data System (ADS)

Wu, Jianing; Yan, Shaoze; Xie, Liyang; Gao, Peng

2012-07-01

The reliability apportionment of spacecraft solar array is of significant importance for spacecraft designers in the early stage of design. However, it is difficult to use the existing methods to resolve reliability apportionment problem because of the data insufficiency and the uncertainty of the relations among the components in the mechanical system. This paper proposes a new method which combines the fuzzy comprehensive evaluation with fuzzy reasoning Petri net (FRPN) to accomplish the reliability apportionment of the solar array. The proposed method extends the previous fuzzy methods and focuses on the characteristics of the subsystems and the intrinsic associations among the components. The analysis results show that the synchronization mechanism may obtain the highest reliability value and the solar panels and hinges may get the lowest reliability before design and manufacturing. Our developed method is of practical significance for the reliability apportionment of solar array where the design information has not been clearly identified, particularly in early stage of design.

Design of a Dissolving Microneedle Platform for Transdermal Delivery of a Fixed-Dose Combination of Cardiovascular Drugs.

PubMed

Quinn, Helen L; Bonham, Louise; Hughes, Carmel M; Donnelly, Ryan F

2015-10-01

Microneedles (MNs) are a minimally invasive drug delivery platform, designed to enhance transdermal drug delivery by breaching the stratum corneum. For the first time, this study describes the simultaneous delivery of a combination of three drugs using a dissolving polymeric MN system. In the present study, aspirin, lisinopril dihydrate, and atorvastatin calcium trihydrate were used as exemplar cardiovascular drugs and formulated into MN arrays using two biocompatible polymers, poly(vinylpyrrollidone) and poly(methylvinylether/maleic acid). Following fabrication, dissolution, mechanical testing, and determination of drug recovery from the MN arrays, in vitro drug delivery studies were undertaken, followed by HPLC analysis. All three drugs were successfully delivered in vitro across neonatal porcine skin, with similar permeation profiles achieved from both polymer formulations. An average of 126.3 ± 18.1 μg of atorvastatin calcium trihydrate was delivered, notably lower than the 687.9 ± 101.3 μg of lisinopril and 3924 ± 1011 μg of aspirin, because of the hydrophobic nature of the atorvastatin molecule and hence poor dissolution from the array. Polymer deposition into the skin may be an issue with repeat application of such a MN array, hence future work will consider more appropriate MN systems for continuous use, alongside tailoring delivery to less hydrophilic compounds. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.

Power-Combined GaN Amplifier with 2.28-W Output Power at 87 GHz

NASA Technical Reports Server (NTRS)

Fung, King Man; Ward, John; Chattopadhyay, Goutam; Lin, Robert H.; Samoska, Lorene A.; Kangaslahti, Pekka P.; Mehdi, Imran; Lambrigtsen, Bjorn H.; Goldsmith, Paul F.; Soria, Mary M.;

Fiber-Optic Array Scanning Technology (FAST) for Detection and Molecular Characterization of Circulating Tumor Cells.

PubMed

Ao, Zheng; Liu, Xiaohe

2017-01-01

Circulating tumor cell (CTC) as an important component in "liquid biopsy" holds crucial clinical relevance in cancer prognosis, treatment efficiency evaluation, prediction and potentially early detection. Here, we present a Fiber-optic Array Scanning Technology (FAST) that enables antigen-agnostic, size-agnostic detection of CTC. By immunofluorescence staining detection of a combination of a panel of markers, FAST technology can be applied to detect rare CTC in non-small cell lung cancer (NSCLC) setting with high sensitivity and specificity. In combination with Automated Digital Microscopy (ADM) platform, companion markers on CTC such as Vimentin and Programmed death-ligand 1 (PD-L1) can also be analyzed to further characterize these CTCs. FAST data output is also compatible with downstream single cell picking platforms. Single cell can be isolated post ADM confirmation and used for "actionable" genetic mutations analysis.

Decoding grating orientation from microelectrode array recordings in monkey cortical area V4.

PubMed

Manyakov, Nikolay V; Van Hulle, Marc M

2010-04-01

We propose an invasive brain-machine interface (BMI) that decodes the orientation of a visual grating from spike train recordings made with a 96 microelectrodes array chronically implanted into the prelunate gyrus (area V4) of a rhesus monkey. The orientation is decoded irrespective of the grating's spatial frequency. Since pyramidal cells are less prominent in visual areas, compared to (pre)motor areas, the recordings contain spikes with smaller amplitudes, compared to the noise level. Hence, rather than performing spike decoding, feature selection algorithms are applied to extract the required information for the decoder. Two types of feature selection procedures are compared, filter and wrapper. The wrapper is combined with a linear discriminant analysis classifier, and the filter is followed by a radial-basis function support vector machine classifier. In addition, since we have a multiclass classification problen, different methods for combining pairwise classifiers are compared.

Standard, Random, and Optimum Array conversions from Two-Pole resistance data

DOE PAGES

Rucker, D. F.; Glaser, Danney R.

2014-09-01

We present an array evaluation of standard and nonstandard arrays over a hydrogeological target. We develop the arrays by linearly combining data from the pole-pole (or 2-pole) array. The first test shows that reconstructed resistances for the standard Schlumberger and dipoledipole arrays are equivalent or superior to the measured arrays in terms of noise, especially at large geometric factors. The inverse models for the standard arrays also confirm what others have presented in terms of target resolvability, namely the dipole-dipole array has the highest resolution. In the second test, we reconstruct random electrode combinations from the 2-pole data segregated intomore » inner, outer, and overlapping dipoles. The resistance data and inverse models from these randomized arrays show those with inner dipoles to be superior in terms of noise and resolution and that overlapping dipoles can cause model instability and low resolution. Finally, we use the 2-pole data to create an optimized array that maximizes the model resolution matrix for a given electrode geometry. The optimized array produces the highest resolution and target detail. Thus, the tests demonstrate that high quality data and high model resolution can be achieved by acquiring field data from the pole-pole array.« less

Modeling change from large-scale high-dimensional spatio-temporal array data

NASA Astrophysics Data System (ADS)

Lu, Meng; Pebesma, Edzer

2014-05-01

The massive data that come from Earth observation satellite and other sensors provide significant information for modeling global change. At the same time, the high dimensionality of the data has brought challenges in data acquisition, management, effective querying and processing. In addition, the output of earth system modeling tends to be data intensive and needs methodologies for storing, validation, analyzing and visualization, e.g. as maps. An important proportion of earth system observations and simulated data can be represented as multi-dimensional array data, which has received increasingly attention in big data management and spatial-temporal analysis. Study cases will be developed in natural science such as climate change, hydrological modeling, sediment dynamics, from which the addressing of big data problems is necessary. Multi-dimensional array-based database management and analytics system such as Rasdaman, SciDB, and R will be applied to these cases. From these studies will hope to learn the strengths and weaknesses of these systems, how they might work together or how semantics of array operations differ, through addressing the problems associated with big data. Research questions include: • How can we reduce dimensions spatially and temporally, or thematically? • How can we extend existing GIS functions to work on multidimensional arrays? • How can we combine data sets of different dimensionality or different resolutions? • Can map algebra be extended to an intelligible array algebra? • What are effective semantics for array programming of dynamic data driven applications? • In which sense are space and time special, as dimensions, compared to other properties? • How can we make the analysis of multi-spectral, multi-temporal and multi-sensor earth observation data easy?

High performance hybrid silicon micropillar solar cell based on light trapping characteristics of Cu nanoparticles

NASA Astrophysics Data System (ADS)

Zhang, Yulong; Fan, Zhiqiang; Zhang, Weijia; Ma, Qiang; Jiang, Zhaoyi; Ma, Denghao

2018-05-01

High performance silicon combined structure (micropillar with Cu nanoparticles) solar cell has been synthesized from N-type silicon substrates based on the micropillar array. The combined structure solar cell exhibited higher short circuit current rather than the silicon miropillar solar cell, which the parameters of micropillar array are the same. Due to the Cu nanoparticles were decorated on the surface of silicon micropillar array, the photovoltaic properties of cells have been improved. In addition, the optimal efficiency of 11.5% was measured for the combined structure solar cell, which is better than the silicon micropillar cell.

Moho Depth and Geometry in the Illinois Basin Region Based on Gravity and Seismic Data from an EarthScope FlexArray Experiment

NASA Astrophysics Data System (ADS)

Curcio, D. D.; Pavlis, G. L.; Yang, X.; Hamburger, M. W.; Zhang, H.; Ravat, D.

2017-12-01

We present results from a combined analysis of seismic and gravity in the Illinois Basin region that demonstrate the presence of an unusually deep and highly variable Moho discontinuity. We construct a new, high-resolution image of the Earth's crust beneath the Illinois Basin using teleseismic P-wave receiver functions from the EarthScope OIINK (Ozarks, Illinois, INdiana, Kentucky) Flexible Array and the USArray Transportable Array. Our seismic analyses involved data from 143 OIINK stations and 80 USArray stations, using 3D plane-wave migration and common conversion point (CCP) stacking of P-to-S conversion data. Seismic interpretation has been done using the seismic exploration software package Petrel. One of the most surprising results is the anomalous depth of the Moho in this area, ranging from 41 to 63 km, with an average depth of 50 km. This thickened crust is unexpected in the Illinois Basin area, which has not been subject to convergence and mountain building processes in the last 900 Ma. This anomalously thick crust in combination with the minimal topography requires abnormally dense lower crust or unusually light upper mantle in order to retain gravitational equilibrium. Combining gravity modeling with the seismically identified Moho and a ubiquitous lower crustal boundary, we solve for the density variation of the middle and lower crust. We test the hypothesis that the anomalously thick crust and its high lower crustal layer observed in most of the central and southeastern Illinois Basin predates the formation and development of the current Illinois Basin. Post-formation tectonic activity, such as late Precambrian rifting or underplating are inferred to have modified the crustal thickness as well. The combination of high-resolution seismic data analysis and gravity modeling promises to provide additional insight into the geometry and composition of the lower crust in the Illinois Basin area.

A mixed-signal implementation of a polychronous spiking neural network with delay adaptation

PubMed Central

Wang, Runchun M.; Hamilton, Tara J.; Tapson, Jonathan C.; van Schaik, André

2014-01-01

We present a mixed-signal implementation of a re-configurable polychronous spiking neural network capable of storing and recalling spatio-temporal patterns. The proposed neural network contains one neuron array and one axon array. Spike Timing Dependent Delay Plasticity is used to fine-tune delays and add dynamics to the network. In our mixed-signal implementation, the neurons and axons have been implemented as both analog and digital circuits. The system thus consists of one FPGA, containing the digital neuron array and the digital axon array, and one analog IC containing the analog neuron array and the analog axon array. The system can be easily configured to use different combinations of each. We present and discuss the experimental results of all combinations of the analog and digital axon arrays and the analog and digital neuron arrays. The test results show that the proposed neural network is capable of successfully recalling more than 85% of stored patterns using both analog and digital circuits. PMID:24672422

A mixed-signal implementation of a polychronous spiking neural network with delay adaptation.

PubMed

Wang, Runchun M; Hamilton, Tara J; Tapson, Jonathan C; van Schaik, André

2014-01-01

We present a mixed-signal implementation of a re-configurable polychronous spiking neural network capable of storing and recalling spatio-temporal patterns. The proposed neural network contains one neuron array and one axon array. Spike Timing Dependent Delay Plasticity is used to fine-tune delays and add dynamics to the network. In our mixed-signal implementation, the neurons and axons have been implemented as both analog and digital circuits. The system thus consists of one FPGA, containing the digital neuron array and the digital axon array, and one analog IC containing the analog neuron array and the analog axon array. The system can be easily configured to use different combinations of each. We present and discuss the experimental results of all combinations of the analog and digital axon arrays and the analog and digital neuron arrays. The test results show that the proposed neural network is capable of successfully recalling more than 85% of stored patterns using both analog and digital circuits.

Promising Results from Three NASA SBIR Solar Array Technology Development Programs

NASA Technical Reports Server (NTRS)

Eskenazi, Mike; White, Steve; Spence, Brian; Douglas, Mark; Glick, Mike; Pavlick, Ariel; Murphy, David; O'Neill, Mark; McDanal, A. J.; Piszczor, Michael

2005-01-01

Results from three NASA SBIR solar array technology programs are presented. The programs discussed are: 1) Thin Film Photovoltaic UltraFlex Solar Array; 2) Low Cost/Mass Electrostatically Clean Solar Array (ESCA); and 3) Stretched Lens Array SquareRigger (SLASR). The purpose of the Thin Film UltraFlex (TFUF) Program is to mature and validate the use of advanced flexible thin film photovoltaics blankets as the electrical subsystem element within an UltraFlex solar array structural system. In this program operational prototype flexible array segments, using United Solar amorphous silicon cells, are being manufactured and tested for the flight qualified UltraFlex structure. In addition, large size (e.g. 10 kW GEO) TFUF wing systems are being designed and analyzed. Thermal cycle and electrical test and analysis results from the TFUF program are presented. The purpose of the second program entitled, Low Cost/Mass Electrostatically Clean Solar Array (ESCA) System, is to develop an Electrostatically Clean Solar Array meeting NASA s design requirements and ready this technology for commercialization and use on the NASA MMS and GED missions. The ESCA designs developed use flight proven materials and processes to create a ESCA system that yields low cost, low mass, high reliability, high power density, and is adaptable to any cell type and coverglass thickness. All program objectives, which included developing specifications, creating ESCA concepts, concept analysis and trade studies, producing detailed designs of the most promising ESCA treatments, manufacturing ESCA demonstration panels, and LEO (2,000 cycles) and GEO (1,350 cycles) thermal cycling testing of the down-selected designs were successfully achieved. The purpose of the third program entitled, "High Power Platform for the Stretched Lens Array," is to develop an extremely lightweight, high efficiency, high power, high voltage, and low stowed volume solar array suitable for very high power (multi-kW to MW) applications. These objectives are achieved by combining two cutting edge technologies, the SquareRigger solar array structure and the Stretched Lens Array (SLA). The SLA SquareRigger solar array is termed SLASR. All program objectives, which included developing specifications, creating preliminary designs for a near-term SLASR, detailed structural, mass, power, and sizing analyses, fabrication and power testing of a functional flight-like SLASR solar blanket, were successfully achieved.

Economic analysis of the unified heliostat array

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1980-11-07

The Unified Heliostat Array (UHA) is comprised of conventional two-axis heliostats mounted on a terraced south-facing wall of a single structure. The arrangement of heliostats on the array is chosen to eliminate or control the degree of inter-heliostat shading and blocking. The UHA was investifated as to cost and optical performance. Two heliostats, the Veda Industrial Heliostat (VIH) and the Repowering Heliostat were investigated in conjunction with the UHA. The UHA was found to be a viable candidate for solar thermal central receiver applications. The UHA-VIH combination was shown to provide very high flux densities and to be suitable formore » high temperature applications in the 1000/sup 0/K to 2000/sup 0/K range. These temperatures were shown to be achievable even with very small (1 MWt) collector fields.« less

High-performance ultra-low power VLSI analog processor for data compression

NASA Technical Reports Server (NTRS)

Tawel, Raoul (Inventor)

1996-01-01

An apparatus for data compression employing a parallel analog processor. The apparatus includes an array of processor cells with N columns and M rows wherein the processor cells have an input device, memory device, and processor device. The input device is used for inputting a series of input vectors. Each input vector is simultaneously input into each column of the array of processor cells in a pre-determined sequential order. An input vector is made up of M components, ones of which are input into ones of M processor cells making up a column of the array. The memory device is used for providing ones of M components of a codebook vector to ones of the processor cells making up a column of the array. A different codebook vector is provided to each of the N columns of the array. The processor device is used for simultaneously comparing the components of each input vector to corresponding components of each codebook vector, and for outputting a signal representative of the closeness between the compared vector components. A combination device is used to combine the signal output from each processor cell in each column of the array and to output a combined signal. A closeness determination device is then used for determining which codebook vector is closest to an input vector from the combined signals, and for outputting a codebook vector index indicating which of the N codebook vectors was the closest to each input vector input into the array.

Unique volatolomic signatures of TP53 and KRAS in lung cells

PubMed Central

Davies, M P A; Barash, O; Jeries, R; Peled, N; Ilouze, M; Hyde, R; Marcus, M W; Field, J K; Haick, H

2014-01-01

Background: Volatile organic compounds (VOCs) are potential biomarkers for cancer detection in breath, but it is unclear if they reflect specific mutations. To test this, we have compared human bronchial epithelial cell (HBEC) cell lines carrying the KRASV12 mutation, knockdown of TP53 or both with parental HBEC cells. Methods: VOC from headspace above cultured cells were collected by passive sampling and analysed by thermal desorption gas chromatography mass spectrometry (TD-GC–MS) or sensor array with discriminant factor analysis (DFA). Results: In TD-GC–MS analysis, individual compounds had limited ability to discriminate between cell lines, but by applying DFA analysis combinations of 20 VOCs successfully discriminated between all cell types (accuracies 80–100%, with leave-one-out cross validation). Sensor array detection DFA demonstrated the ability to discriminate samples based on their cell type for all comparisons with accuracies varying between 77% and 93%. Conclusions: Our results demonstrate that minimal genetic changes in bronchial airway cells lead to detectable differences in levels of specific VOCs identified by TD-GC–MS or of patterns of VOCs identified by sensor array output. From the clinical aspect, these results suggest the possibility of breath analysis for detection of minimal genetic changes for earlier diagnosis or for genetic typing of lung cancers. PMID:25051409

Restoring Low Sidelobe Antenna Patterns with Failed Elements in a Phased Array Antenna

DTIC Science & Technology

2016-02-01

optimum low sidelobes are demonstrated in several examples. Index Terms — Array signal processing, beams, linear algebra , phased arrays, shaped...represented by a linear combination of low sidelobe beamformers with no failed elements, ’s, in a neighborhood around under the constraint that the linear ...would expect that linear combinations of them in a neighborhood around would also have low sidelobes. The algorithms in this paper exploit this

Certified dual-corrected radiation patterns of phased antenna arrays by offline–online order reduction of finite-element models

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sommer, A., E-mail: a.sommer@lte.uni-saarland.de; Farle, O., E-mail: o.farle@lte.uni-saarland.de; Dyczij-Edlinger, R., E-mail: edlinger@lte.uni-saarland.de

2015-10-15

This paper presents a fast numerical method for computing certified far-field patterns of phased antenna arrays over broad frequency bands as well as wide ranges of steering and look angles. The proposed scheme combines finite-element analysis, dual-corrected model-order reduction, and empirical interpolation. To assure the reliability of the results, improved a posteriori error bounds for the radiated power and directive gain are derived. Both the reduced-order model and the error-bounds algorithm feature offline–online decomposition. A real-world example is provided to demonstrate the efficiency and accuracy of the suggested approach.

A Multipath Mitigation Algorithm for vehicle with Smart Antenna

NASA Astrophysics Data System (ADS)

Ji, Jing; Zhang, Jiantong; Chen, Wei; Su, Deliang

2018-01-01

In this paper, the antenna array adaptive method is used to eliminate the multipath interference in the environment of GPS L1 frequency. Combined with the power inversion (PI) algorithm and the minimum variance no distortion response (MVDR) algorithm, the anti-Simulation and verification of the antenna array, and the program into the FPGA, the actual test on the CBD road, the theoretical analysis of the LCMV criteria and PI and MVDR algorithm principles and characteristics of MVDR algorithm to verify anti-multipath interference performance is better than PI algorithm, The satellite navigation in the field of vehicle engineering practice has some guidance and reference.

Propagation of coherently combined truncated laser beam arrays with beam distortions in non-Kolmogorov turbulence.

PubMed

Tao, Rumao; Si, Lei; Ma, Yanxing; Zhou, Pu; Liu, Zejin

2012-08-10

The propagation properties of coherently combined truncated laser beam arrays with beam distortions through non-Kolmogorov turbulence are studied in detail both analytically and numerically. The analytical expressions for the average intensity and the beam width of coherently combined truncated laser beam arrays with beam distortions propagating through turbulence are derived based on the combination of statistical optics methods and the extended Huygens-Fresnel principle. The effect of beam distortions, such as amplitude modulation and phase fluctuation, is studied by numerical examples. The numerical results reveal that phase fluctuations have significant influence on the spreading of coherently combined truncated laser beam arrays in non-Kolmogorov turbulence, and the effects of the phase fluctuations can be negligible as long as the phase fluctuations are controlled under a certain level, i.e., a>0.05 for the situation considered in the paper. Furthermore, large phase fluctuations can convert the beam distribution rapidly to a Gaussian form, vary the spreading, weaken the optimum truncation effects, and suppress the dependence of spreading on the parameters of the non-Kolmogorov turbulence.

BEAT: Bioinformatics Exon Array Tool to store, analyze and visualize Affymetrix GeneChip Human Exon Array data from disease experiments

PubMed Central

2012-01-01

Background It is known from recent studies that more than 90% of human multi-exon genes are subject to Alternative Splicing (AS), a key molecular mechanism in which multiple transcripts may be generated from a single gene. It is widely recognized that a breakdown in AS mechanisms plays an important role in cellular differentiation and pathologies. Polymerase Chain Reactions, microarrays and sequencing technologies have been applied to the study of transcript diversity arising from alternative expression. Last generation Affymetrix GeneChip Human Exon 1.0 ST Arrays offer a more detailed view of the gene expression profile providing information on the AS patterns. The exon array technology, with more than five million data points, can detect approximately one million exons, and it allows performing analyses at both gene and exon level. In this paper we describe BEAT, an integrated user-friendly bioinformatics framework to store, analyze and visualize exon arrays datasets. It combines a data warehouse approach with some rigorous statistical methods for assessing the AS of genes involved in diseases. Meta statistics are proposed as a novel approach to explore the analysis results. BEAT is available at http://beat.ba.itb.cnr.it. Results BEAT is a web tool which allows uploading and analyzing exon array datasets using standard statistical methods and an easy-to-use graphical web front-end. BEAT has been tested on a dataset with 173 samples and tuned using new datasets of exon array experiments from 28 colorectal cancer and 26 renal cell cancer samples produced at the Medical Genetics Unit of IRCCS Casa Sollievo della Sofferenza. To highlight all possible AS events, alternative names, accession Ids, Gene Ontology terms and biochemical pathways annotations are integrated with exon and gene level expression plots. The user can customize the results choosing custom thresholds for the statistical parameters and exploiting the available clinical data of the samples for a multivariate AS analysis. Conclusions Despite exon array chips being widely used for transcriptomics studies, there is a lack of analysis tools offering advanced statistical features and requiring no programming knowledge. BEAT provides a user-friendly platform for a comprehensive study of AS events in human diseases, displaying the analysis results with easily interpretable and interactive tables and graphics. PMID:22536968

Dielectrophoresis-Assisted Raman Spectroscopy of Intravesicular Analytes on Metallic Pyramids.

PubMed

Barik, Avijit; Cherukulappurath, Sudhir; Wittenberg, Nathan J; Johnson, Timothy W; Oh, Sang-Hyun

2016-02-02

Chemical analysis of membrane-bound containers such as secretory vesicles, organelles, and exosomes can provide insights into subcellular biology. These containers are loaded with a range of important biomolecules, which further underscores the need for sensitive and selective analysis methods. Here we present a metallic pyramid array for intravesicular analysis by combining site-selective dielectrophoresis (DEP) and Raman spectroscopy. Sharp pyramidal tips act as a gradient force generator to trap nanoparticles or vesicles from the solution, and the tips are illuminated by a monochromatic light source for concurrent spectroscopic detection of trapped analytes. The parameters suitable for DEP trapping were optimized by fluorescence microscopy, and the Raman spectroscopy setup was characterized by a nanoparticle based model system. Finally, vesicles loaded with 4-mercaptopyridine were concentrated at the tips and their Raman spectra were detected in real time. These pyramidal tips can perform large-area array-based trapping and spectroscopic analysis, opening up possibilities to detect molecules inside cells or cell-derived vesicles.

A transient plasticity study and low cycle fatigue analysis of the Space Station Freedom photovoltaic solar array blanket

NASA Technical Reports Server (NTRS)

Armand, Sasan C.; Liao, Mei-Hwa; Morris, Ronald W.

1990-01-01

The Space Station Freedom photovoltaic solar array blanket assembly is comprised of several layers of materials having dissimilar elastic, thermal, and mechanical properties. The operating temperature of the solar array, which ranges from -75 to +60 C, along with the material incompatibility of the blanket assembly components combine to cause an elastic-plastic stress in the weld points of the assembly. The weld points are secondary structures in nature, merely serving as electrical junctions for gathering the current. The thermal mechanical loading of the blanket assembly operating in low earth orbit continually changes throughout each 90 min orbit, which raises the possibility of fatigue induced failure. A series of structural analyses were performed in an attempt to predict the fatigue life of the solar cell in the Space Station Freedom photovoltaic array blanket. A nonlinear elastic-plastic MSC/NASTRAN analysis followed by a fatigue calculation indicated a fatigue life of 92,000 to 160,000 cycles for the solar cell weld tabs. Additional analyses predict a permanent buckling phenomenon in the copper interconnect after the first loading cycle. This should reduce or eliminate the pulling of the copper interconnect on the joint where it is welded to the silicon solar cell. It is concluded that the actual fatigue life of the solar array blanket assembly should be significantly higher than the calculated 92,000 cycles, and thus the program requirement of 87,500 cycles (orbits) will be met. Another important conclusion that can be drawn from the overall analysis is that, the strain results obtained from the MSC/NASTRAN nonlinear module are accurate to use for low-cycle fatigue analysis, since both thermal cycle testing of solar cells and analysis have shown higher fatigue life than the minimum program requirement of 87,500 cycles.

Integrated analysis of copy number alteration and RNA expression profiles of cancer using a high-resolution whole-genome oligonucleotide array.

PubMed

Jung, Seung-Hyun; Shin, Seung-Hun; Yim, Seon-Hee; Choi, Hye-Sun; Lee, Sug-Hyung; Chung, Yeun-Jun

2009-07-31

Recently, microarray-based comparative genomic hybridization (array-CGH) has emerged as a very efficient technology with higher resolution for the genome-wide identification of copy number alterations (CNA). Although CNAs are thought to affect gene expression, there is no platform currently available for the integrated CNA-expression analysis. To achieve high-resolution copy number analysis integrated with expression profiles, we established human 30k oligoarray-based genome-wide copy number analysis system and explored the applicability of this system for integrated genome and transcriptome analysis using MDA-MB-231 cell line. We compared the CNAs detected by the oligoarray with those detected by the 3k BAC array for validation. The oligoarray identified the single copy difference more accurately and sensitively than the BAC array. Seventeen CNAs detected by both platforms in MDA-MB-231 such as gains of 5p15.33-13.1, 8q11.22-8q21.13, 17p11.2, and losses of 1p32.3, 8p23.3-8p11.21, and 9p21 were consistently identified in previous studies on breast cancer. There were 122 other small CNAs (mean size 1.79 mb) that were detected by oligoarray only, not by BAC-array. We performed genomic qPCR targeting 7 CNA regions, detected by oligoarray only, and one non-CNA region to validate the oligoarray CNA detection. All qPCR results were consistent with the oligoarray-CGH results. When we explored the possibility of combined interpretation of both DNA copy number and RNA expression profiles, mean DNA copy number and RNA expression levels showed a significant correlation. In conclusion, this 30k oligoarray-CGH system can be a reasonable choice for analyzing whole genome CNAs and RNA expression profiles at a lower cost.

Optimizing Satellite Communications With Adaptive and Phased Array Antennas

NASA Technical Reports Server (NTRS)

Ingram, Mary Ann; Romanofsky, Robert; Lee, Richard Q.; Miranda, Felix; Popovic, Zoya; Langley, John; Barott, William C.; Ahmed, M. Usman; Mandl, Dan

2004-01-01

A new adaptive antenna array architecture for low-earth-orbiting satellite ground stations is being investigated. These ground stations are intended to have no moving parts and could potentially be operated in populated areas, where terrestrial interference is likely. The architecture includes multiple, moderately directive phased arrays. The phased arrays, each steered in the approximate direction of the satellite, are adaptively combined to enhance the Signal-to-Noise and Interference-Ratio (SNIR) of the desired satellite. The size of each phased array is to be traded-off with the number of phased arrays, to optimize cost, while meeting a bit-error-rate threshold. Also, two phased array architectures are being prototyped: a spacefed lens array and a reflect-array. If two co-channel satellites are in the field of view of the phased arrays, then multi-user detection techniques may enable simultaneous demodulation of the satellite signals, also known as Space Division Multiple Access (SDMA). We report on Phase I of the project, in which fixed directional elements are adaptively combined in a prototype to demodulate the S-band downlink of the EO-1 satellite, which is part of the New Millennium Program at NASA.

Xray: N-dimensional, labeled arrays for analyzing physical datasets in Python

NASA Astrophysics Data System (ADS)

Hoyer, S.

2015-12-01

Efficient analysis of geophysical datasets requires tools that both preserve and utilize metadata, and that transparently scale to process large datas. Xray is such a tool, in the form of an open source Python library for analyzing the labeled, multi-dimensional array (tensor) datasets that are ubiquitous in the Earth sciences. Xray's approach pairs Python data structures based on the data model of the netCDF file format with the proven design and user interface of pandas, the popular Python data analysis library for labeled tabular data. On top of the NumPy array, xray adds labeled dimensions (e.g., "time") and coordinate values (e.g., "2015-04-10"), which it uses to enable a host of operations powered by these labels: selection, aggregation, alignment, broadcasting, split-apply-combine, interoperability with pandas and serialization to netCDF/HDF5. Many of these operations are enabled by xray's tight integration with pandas. Finally, to allow for easy parallelism and to enable its labeled data operations to scale to datasets that does not fit into memory, xray integrates with the parallel processing library dask.

Functional Analysis With a Barcoder Yeast Gene Overexpression System

PubMed Central

Douglas, Alison C.; Smith, Andrew M.; Sharifpoor, Sara; Yan, Zhun; Durbic, Tanja; Heisler, Lawrence E.; Lee, Anna Y.; Ryan, Owen; Göttert, Hendrikje; Surendra, Anu; van Dyk, Dewald; Giaever, Guri; Boone, Charles; Nislow, Corey; Andrews, Brenda J.

2012-01-01

Systematic analysis of gene overexpression phenotypes provides an insight into gene function, enzyme targets, and biological pathways. Here, we describe a novel functional genomics platform that enables a highly parallel and systematic assessment of overexpression phenotypes in pooled cultures. First, we constructed a genome-level collection of ~5100 yeast barcoder strains, each of which carries a unique barcode, enabling pooled fitness assays with a barcode microarray or sequencing readout. Second, we constructed a yeast open reading frame (ORF) galactose-induced overexpression array by generating a genome-wide set of yeast transformants, each of which carries an individual plasmid-born and sequence-verified ORF derived from the Saccharomyces cerevisiae full-length EXpression-ready (FLEX) collection. We combined these collections genetically using synthetic genetic array methodology, generating ~5100 strains, each of which is barcoded and overexpresses a specific ORF, a set we termed “barFLEX.” Additional synthetic genetic array allows the barFLEX collection to be moved into different genetic backgrounds. As a proof-of-principle, we describe the properties of the barFLEX overexpression collection and its application in synthetic dosage lethality studies under different environmental conditions. PMID:23050238

Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome.

PubMed

Markert, Sebastian Matthias; Britz, Sebastian; Proppert, Sven; Lang, Marietta; Witvliet, Daniel; Mulcahy, Ben; Sauer, Markus; Zhen, Mei; Bessereau, Jean-Louis; Stigloher, Christian

2016-10-01

Correlating molecular labeling at the ultrastructural level with high confidence remains challenging. Array tomography (AT) allows for a combination of fluorescence and electron microscopy (EM) to visualize subcellular protein localization on serial EM sections. Here, we describe an application for AT that combines near-native tissue preservation via high-pressure freezing and freeze substitution with super-resolution light microscopy and high-resolution scanning electron microscopy (SEM) analysis on the same section. We established protocols that combine SEM with structured illumination microscopy (SIM) and direct stochastic optical reconstruction microscopy (dSTORM). We devised a method for easy, precise, and unbiased correlation of EM images and super-resolution imaging data using endogenous cellular landmarks and freely available image processing software. We demonstrate that these methods allow us to identify and label gap junctions in Caenorhabditis elegans with precision and confidence, and imaging of even smaller structures is feasible. With the emergence of connectomics, these methods will allow us to fill in the gap-acquiring the correlated ultrastructural and molecular identity of electrical synapses.

Comprehensive high-resolution genomic profiling and cytogenetics of human chondrocyte cultures by GTG-banding, locus-specific FISH, SKY and SNP array.

PubMed

Wallenborn, M; Petters, O; Rudolf, D; Hantmann, H; Richter, M; Ahnert, P; Rohani, L; Smink, J J; Bulwin, G C; Krupp, W; Schulz, R M; Holland, H

2018-04-23

In the development of cell-based medicinal products, it is crucial to guarantee that the application of such an advanced therapy medicinal product (ATMP) is safe for the patients. The consensus of the European regulatory authorities is: "In conclusion, on the basis of the state of art, conventional karyotyping can be considered a valuable and useful technique to analyse chromosomal stability during preclinical studies". 408 chondrocyte samples (84 monolayers and 324 spheroids) from six patients were analysed using trypsin-Giemsa staining, spectral karyotyping and fluorescence in situ hybridisation, to evaluate the genetic stability of chondrocyte samples from non-clinical studies. Single nucleotide polymorphism (SNP) array analysis was performed on chondrocyte spheroids from five of the six donors. Applying this combination of techniques, the genetic analyses performed revealed no significant genetic instability until passage 3 in monolayer cells and interphase cells from spheroid cultures at different time points. Clonal occurrence of polyploid metaphases and endoreduplications were identified associated with prolonged cultivation time. Also, gonosomal losses were observed in chondrocyte spheroids, with increasing passage and duration of the differentiation phase. Interestingly, in one of the donors, chromosomal aberrations that are also described in extraskeletal myxoid chondrosarcoma were identified. The SNP array analysis exhibited chromosomal aberrations in two donors and copy neutral losses of heterozygosity regions in four donors. This study showed the necessity of combined genetic analyses at defined cultivation time points in quality studies within the field of cell therapy.

Microarray R-based analysis of complex lysate experiments with MIRACLE

PubMed Central

List, Markus; Block, Ines; Pedersen, Marlene Lemvig; Christiansen, Helle; Schmidt, Steffen; Thomassen, Mads; Tan, Qihua; Baumbach, Jan; Mollenhauer, Jan

2014-01-01

Motivation: Reverse-phase protein arrays (RPPAs) allow sensitive quantification of relative protein abundance in thousands of samples in parallel. Typical challenges involved in this technology are antibody selection, sample preparation and optimization of staining conditions. The issue of combining effective sample management and data analysis, however, has been widely neglected. Results: This motivated us to develop MIRACLE, a comprehensive and user-friendly web application bridging the gap between spotting and array analysis by conveniently keeping track of sample information. Data processing includes correction of staining bias, estimation of protein concentration from response curves, normalization for total protein amount per sample and statistical evaluation. Established analysis methods have been integrated with MIRACLE, offering experimental scientists an end-to-end solution for sample management and for carrying out data analysis. In addition, experienced users have the possibility to export data to R for more complex analyses. MIRACLE thus has the potential to further spread utilization of RPPAs as an emerging technology for high-throughput protein analysis. Availability: Project URL: http://www.nanocan.org/miracle/ Contact: mlist@health.sdu.dk Supplementary information: Supplementary data are available at Bioinformatics online. PMID:25161257

Microarray R-based analysis of complex lysate experiments with MIRACLE.

PubMed

List, Markus; Block, Ines; Pedersen, Marlene Lemvig; Christiansen, Helle; Schmidt, Steffen; Thomassen, Mads; Tan, Qihua; Baumbach, Jan; Mollenhauer, Jan

2014-09-01

Reverse-phase protein arrays (RPPAs) allow sensitive quantification of relative protein abundance in thousands of samples in parallel. Typical challenges involved in this technology are antibody selection, sample preparation and optimization of staining conditions. The issue of combining effective sample management and data analysis, however, has been widely neglected. This motivated us to develop MIRACLE, a comprehensive and user-friendly web application bridging the gap between spotting and array analysis by conveniently keeping track of sample information. Data processing includes correction of staining bias, estimation of protein concentration from response curves, normalization for total protein amount per sample and statistical evaluation. Established analysis methods have been integrated with MIRACLE, offering experimental scientists an end-to-end solution for sample management and for carrying out data analysis. In addition, experienced users have the possibility to export data to R for more complex analyses. MIRACLE thus has the potential to further spread utilization of RPPAs as an emerging technology for high-throughput protein analysis. Project URL: http://www.nanocan.org/miracle/. Supplementary data are available at Bioinformatics online. © The Author 2014. Published by Oxford University Press.

Sequencing of oligosaccharides using enzyme array digestion with electrochemical and fluorescent detections

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sun, M.; Lee, C.S.

1997-12-31

The objective of this study is to develop a rapid and sensitive method for oligosaccharide sequencing. The oligosaccharides are subjected to the enzyme array digestion with exoglycosidases of known and well-defined specificities. The enzyme array method involves the division of oligosaccharide sample into aliquots, and the incubation of each aliquot with a precisely defined mixture of exoglycosidases. In the enzyme array method, the presence of a specific linkage anywhere in the oligosaccharide is determined by the inability of an enzyme mixture lacking a given enzyme to cleave that linkage ( a stop point) and the ability of the other enzymesmore » to cleave the linkage up to that point. The direct quantification of released monosaccharides from the enzyme array can be achieved by using pulsed amperometric detection (PAD) or by fluorescent derivatization with a fluorophoric agent. The measured monosaccharide concentrations in combination with the enzyme array analysis provide detail characterization of oligosaccharides with their sugar composition, configuration, and linkage information, The released monosaccharides are further quantified by anion exchange chromatography and capillary electrophoresis for the comparison with the results obtained from PAD and fluorescence measurements. Our enzyme array-electrochemical (or fluorescent) detection method does not require any separation procedure and any prior labeling of oligosaccharide and have several practical advantages over the current carbohydrate sequencing techniques including simplicity, speed, and the ability to use small amounts of starting material.« less

Broadband acoustic phased array with subwavelength active tube array

NASA Astrophysics Data System (ADS)

Li, Xiao-Yan; Yang, Zhang-Zhao; Zhu, Yi-Fan; Zou, Xin-Ye; Cheng, Jian-Chun

2018-02-01

Acoustic metasurfaces provide a way to manipulate wavefronts at anomalous reflection or refraction angles through subwavelength structures. Here, based on the generalized Snell's refraction law for acoustic metasurfaces and the classical acoustic phased array (PA) theory, a broadband acoustic PA with a subwavelength active tube array has been proposed to form a special acoustic beam and to determine the directivity characteristics of the acoustic source. Theoretical analysis shows that the dispersionless wavefront manipulation can be realized by the gradient model of the active tube array, and a wide working frequency band can be obtained in practical applications from the simulated and experimental results. The numerical results of forming a special acoustic beam and establishing an acoustic focus model with an arbitrary focal position are consistent with the theoretical predictions. The experimental results agree well with the simulated results in the model of forming the acoustic beam of 45 ° . By combining acoustic metamaterials and conventional acoustic PA, the model of the active tube array paves a way to design a composite acoustic PA with high radiation efficiency and system robustness without the need for any complex circuit control system. This design concept is expected to be used in the design of ultrasonic therapy devices and high-efficiency transducers.

Optimizing fixed observational assets in a coastal observatory

NASA Astrophysics Data System (ADS)

Frolov, Sergey; Baptista, António; Wilkin, Michael

2008-11-01

Proliferation of coastal observatories necessitates an objective approach to managing of observational assets. In this article, we used our experience in the coastal observatory for the Columbia River estuary and plume to identify and address common problems in managing of fixed observational assets, such as salinity, temperature, and water level sensors attached to pilings and moorings. Specifically, we addressed the following problems: assessing the quality of an existing array, adding stations to an existing array, removing stations from an existing array, validating an array design, and targeting of an array toward data assimilation or monitoring. Our analysis was based on a combination of methods from oceanographic and statistical literature, mainly on the statistical machinery of the best linear unbiased estimator. The key information required for our analysis was the covariance structure for a field of interest, which was computed from the output of assimilated and non-assimilated models of the Columbia River estuary and plume. The network optimization experiments in the Columbia River estuary and plume proved to be successful, largely withstanding the scrutiny of sensitivity and validation studies, and hence providing valuable insight into optimization and operation of the existing observational network. Our success in the Columbia River estuary and plume suggest that algorithms for optimal placement of sensors are reaching maturity and are likely to play a significant role in the design of emerging ocean observatories, such as the United State's ocean observation initiative (OOI) and integrated ocean observing system (IOOS) observatories, and smaller regional observatories.

Reconfigurable Antennas for High Data Rate Multi-beam Communication Systems

NASA Technical Reports Server (NTRS)

Bernhard, Jennifer T.; Michielssen, Eric

2005-01-01

High-speed (2-100 Mb/sec) wireless data communication - whether land- or satellite-based - faces a major challenge: high error rates caused by interference and unpredictable environments. A planar antenna system that can be reconfigured to respond to changing conditions has the potential to dramatically improve data throughput and system reliability. Moreover, new planar antenna designs that reduce array size, weight, and cost can have a significant impact on terrestrial and satellite communication system performance. This research developed new individually-reconfigurable planar antenna array elements that can be adjusted to provide multiple beams while providing increased scan angles and higher aperture efficiency than traditional diffraction-limited arrays. These new elements are microstrip spiral antennas with specialized tuning mechanisms that provide adjustable radiation patterns. We anticipate that these new elements can be used in both large and small arrays for inter-satellite communication as well as tracking of multiple mobile surface-based units. Our work has developed both theoretical descriptions as well as experimental prototypes of the antennas in both single element and array embodiments. The technical summary of the results of this work is divided into six sections: A. Cavity model for analysis and design of pattern reconfigurable antennas; B. Performance of antenna in array configurations for broadside and endfire operation; C. Performance of antenna in array configurations for beam scanning operation; D. Simulation of antennas in infinite phased arrays; E. Demonstration of antenna with commercially-available RF MEMS switches; F. Design of antenna MEMS switch combinations for direct simultaneous fabrication.

The X-IFU end-to-end simulations performed for the TES array optimization exercise

NASA Astrophysics Data System (ADS)

Peille, Philippe; Wilms, J.; Brand, T.; Cobo, B.; Ceballos, M. T.; Dauser, T.; Smith, S. J.; Barret, D.; den Herder, J. W.; Piro, L.; Barcons, X.; Pointecouteau, E.; Bandler, S.; den Hartog, R.; de Plaa, J.

2015-09-01

The focal plane assembly of the Athena X-ray Integral Field Unit (X-IFU) includes as the baseline an array of ~4000 single size calorimeters based on Transition Edge Sensors (TES). Other sensor array configurations could however be considered, combining TES of different properties (e.g. size). In attempting to improve the X-IFU performance in terms of field of view, count rate performance, and even spectral resolution, two alternative TES array configurations to the baseline have been simulated, each combining a small and a large pixel array. With the X-IFU end-to-end simulator, a sub-sample of the Athena core science goals, selected by the X-IFU science team as potentially driving the optimal TES array configuration, has been simulated for the results to be scientifically assessed and compared. In this contribution, we will describe the simulation set-up for the various array configurations, and highlight some of the results of the test cases simulated.

Hybrid Arrays for Chemical Sensing

NASA Astrophysics Data System (ADS)

Kramer, Kirsten E.; Rose-Pehrsson, Susan L.; Johnson, Kevin J.; Minor, Christian P.

In recent years, multisensory approaches to environment monitoring for chemical detection as well as other forms of situational awareness have become increasingly popular. A hybrid sensor is a multimodal system that incorporates several sensing elements and thus produces data that are multivariate in nature and may be significantly increased in complexity compared to data provided by single-sensor systems. Though a hybrid sensor is itself an array, hybrid sensors are often organized into more complex sensing systems through an assortment of network topologies. Part of the reason for the shift to hybrid sensors is due to advancements in sensor technology and computational power available for processing larger amounts of data. There is also ample evidence to support the claim that a multivariate analytical approach is generally superior to univariate measurements because it provides additional redundant and complementary information (Hall, D. L.; Linas, J., Eds., Handbook of Multisensor Data Fusion, CRC, Boca Raton, FL, 2001). However, the benefits of a multisensory approach are not automatically achieved. Interpretation of data from hybrid arrays of sensors requires the analyst to develop an application-specific methodology to optimally fuse the disparate sources of data generated by the hybrid array into useful information characterizing the sample or environment being observed. Consequently, multivariate data analysis techniques such as those employed in the field of chemometrics have become more important in analyzing sensor array data. Depending on the nature of the acquired data, a number of chemometric algorithms may prove useful in the analysis and interpretation of data from hybrid sensor arrays. It is important to note, however, that the challenges posed by the analysis of hybrid sensor array data are not unique to the field of chemical sensing. Applications in electrical and process engineering, remote sensing, medicine, and of course, artificial intelligence and robotics, all share the same essential data fusion challenges. The design of a hybrid sensor array should draw on this extended body of knowledge. In this chapter, various techniques for data preprocessing, feature extraction, feature selection, and modeling of sensor data will be introduced and illustrated with data fusion approaches that have been implemented in applications involving data from hybrid arrays. The example systems discussed in this chapter involve the development of prototype sensor networks for damage control event detection aboard US Navy vessels and the development of analysis algorithms to combine multiple sensing techniques for enhanced remote detection of unexploded ordnance (UXO) in both ground surveys and wide area assessments.

Fiber array based hyperspectral Raman imaging for chemical selective analysis of malaria-infected red blood cells.

PubMed

Brückner, Michael; Becker, Katja; Popp, Jürgen; Frosch, Torsten

2015-09-24

A new setup for Raman spectroscopic wide-field imaging is presented. It combines the advantages of a fiber array based spectral translator with a tailor-made laser illumination system for high-quality Raman chemical imaging of sensitive biological samples. The Gaussian-like intensity distribution of the illuminating laser beam is shaped by a square-core optical multimode fiber to a top-hat profile with very homogeneous intensity distribution to fulfill the conditions of Koehler. The 30 m long optical fiber and an additional vibrator efficiently destroy the polarization and coherence of the illuminating light. This homogeneous, incoherent illumination is an essential prerequisite for stable quantitative imaging of complex biological samples. The fiber array translates the two-dimensional lateral information of the Raman stray light into separated spectral channels with very high contrast. The Raman image can be correlated with a corresponding white light microscopic image of the sample. The new setup enables simultaneous quantification of all Raman spectra across the whole spatial area with very good spectral resolution and thus outperforms other Raman imaging approaches based on scanning and tunable filters. The unique capabilities of the setup for fast, gentle, sensitive, and selective chemical imaging of biological samples were applied for automated hemozoin analysis. A special algorithm was developed to generate Raman images based on the hemozoin distribution in red blood cells without any influence from other Raman scattering. The new imaging setup in combination with the robust algorithm provides a novel, elegant way for chemical selective analysis of the malaria pigment hemozoin in early ring stages of Plasmodium falciparum infected erythrocytes. Copyright © 2015 Elsevier B.V. All rights reserved.

Phased Antenna Array for Global Navigation Satellite System Signals

NASA Technical Reports Server (NTRS)

Turbiner, Dmitry (Inventor)

2015-01-01

Systems and methods for phased array antennas are described. Supports for phased array antennas can be constructed by 3D printing. The array elements and combiner network can be constructed by conducting wire. Different parameters of the antenna, like the gain and directivity, can be controlled by selection of the appropriate design, and by electrical steering. Phased array antennas may be used for radio occultation measurements.

EzArray: A web-based highly automated Affymetrix expression array data management and analysis system

PubMed Central

Zhu, Yuerong; Zhu, Yuelin; Xu, Wei

2008-01-01

Background Though microarray experiments are very popular in life science research, managing and analyzing microarray data are still challenging tasks for many biologists. Most microarray programs require users to have sophisticated knowledge of mathematics, statistics and computer skills for usage. With accumulating microarray data deposited in public databases, easy-to-use programs to re-analyze previously published microarray data are in high demand. Results EzArray is a web-based Affymetrix expression array data management and analysis system for researchers who need to organize microarray data efficiently and get data analyzed instantly. EzArray organizes microarray data into projects that can be analyzed online with predefined or custom procedures. EzArray performs data preprocessing and detection of differentially expressed genes with statistical methods. All analysis procedures are optimized and highly automated so that even novice users with limited pre-knowledge of microarray data analysis can complete initial analysis quickly. Since all input files, analysis parameters, and executed scripts can be downloaded, EzArray provides maximum reproducibility for each analysis. In addition, EzArray integrates with Gene Expression Omnibus (GEO) and allows instantaneous re-analysis of published array data. Conclusion EzArray is a novel Affymetrix expression array data analysis and sharing system. EzArray provides easy-to-use tools for re-analyzing published microarray data and will help both novice and experienced users perform initial analysis of their microarray data from the location of data storage. We believe EzArray will be a useful system for facilities with microarray services and laboratories with multiple members involved in microarray data analysis. EzArray is freely available from . PMID:18218103

Development and Calibration of a Field-Deployable Microphone Phased Array for Propulsion and Airframe Noise Flyover Measurements

NASA Technical Reports Server (NTRS)

Humphreys, William M., Jr.; Lockard, David P.; Khorrami, Mehdi R.; Culliton, William G.; McSwain, Robert G.; Ravetta, Patricio A.; Johns, Zachary

2016-01-01

A new aeroacoustic measurement capability has been developed consisting of a large channelcount, field-deployable microphone phased array suitable for airframe noise flyover measurements for a range of aircraft types and scales. The array incorporates up to 185 hardened, weather-resistant sensors suitable for outdoor use. A custom 4-mA current loop receiver circuit with temperature compensation was developed to power the sensors over extended cable lengths with minimal degradation of the signal to noise ratio and frequency response. Extensive laboratory calibrations and environmental testing of the sensors were conducted to verify the design's performance specifications. A compact data system combining sensor power, signal conditioning, and digitization was assembled for use with the array. Complementing the data system is a robust analysis system capable of near real-time presentation of beamformed and deconvolved contour plots and integrated spectra obtained from array data acquired during flyover passes. Additional instrumentation systems needed to process the array data were also assembled. These include a commercial weather station and a video monitoring / recording system. A detailed mock-up of the instrumentation suite (phased array, weather station, and data processor) was performed in the NASA Langley Acoustic Development Laboratory to vet the system performance. The first deployment of the system occurred at Finnegan Airfield at Fort A.P. Hill where the array was utilized to measure the vehicle noise from a number of sUAS (small Unmanned Aerial System) aircraft. A unique in-situ calibration method for the array microphones using a hovering aerial sound source was attempted for the first time during the deployment.

Graphene Microcapsule Arrays for Combinatorial Electron Microscopy and Spectroscopy in Liquids

DOE PAGES

Yulaev, Alexander; Guo, Hongxuan; Strelcov, Evgheni; ...

2017-04-27

Atomic-scale thickness, molecular impermeability, low atomic number, and mechanical strength make graphene an ideal electron-transparent membrane for material characterization in liquids and gases with scanning electron microscopy and spectroscopy. Here in this paper, we present a novel sample platform made of an array of thousands of identical isolated graphene-capped microchannels with high aspect ratio. A combination of a global wide field of view with high resolution local imaging of the array allows for high throughput in situ studies as well as for combinatorial screening of solutions, liquid interfaces, and immersed samples. We demonstrate the capabilities of this platform by studyingmore » a pure water sample in comparison with alkali halide solutions, a model electrochemical plating process, and beam-induced crystal growth in liquid electrolyte. Spectroscopic characterization of liquid interfaces and immersed objects with Auger and X-ray fluorescence analysis through the graphene membrane are also demonstrated.« less

Opportunistic Beamforming with Wireless Powered 1-bit Feedback Through Rectenna Array

NASA Astrophysics Data System (ADS)

Krikidis, Ioannis

2015-11-01

This letter deals with the opportunistic beamforming (OBF) scheme for multi-antenna downlink with spatial randomness. In contrast to conventional OBF, the terminals return only 1-bit feedback, which is powered by wireless power transfer through a rectenna array. We study two fundamental topologies for the combination of the rectenna elements; the direct-current combiner and the radio-frequency combiner. The beam outage probability is derived in closed form for both combination schemes, by using high order statistics and stochastic geometry.

Gravity and magnetic anomaly data analysis

NASA Technical Reports Server (NTRS)

Braile, L. W.; Hinze, W. J.; Vonfrese, R. R. B. (Principal Investigator)

1982-01-01

Progress on the analysis MAGSAT data is reported. The MAGSAT data from 40 deg S to 70 deg N latitude and 30 deg W to 60 E longitude was reduced to radial polarization. In addition, gravity anomaly data from this area were processed and a variety of filtered maps were prepared for combined interpretation of the gravity and magnetic data in conjunction with structural and tectonic maps of the area. The VERSATEC listings and cross-reference maps of variable and array names for the spherical Earth analysis programs NVERTSM, SMFLD, NVERTG, and GFLD were also prepared.

Advanced solar panel designs

NASA Technical Reports Server (NTRS)

Ralph, E. L.; Linder, E.

1995-01-01

This paper describes solar cell panel designs that utilize new hgih efficiency solar cells along with lightweight rigid panel technology. The resulting designs push the W/kg and W/sq m parameters to new high levels. These new designs are well suited to meet the demand for higher performance small satellites. This paper reports on progress made on two SBIR Phase 1 contracts. One panel design involved the use of large area (5.5 cm x 6.5 cm) GaAs/Ge solar cells of 19% efficiency combined with a lightweight rigid graphite fiber epoxy isogrid substrate configuration. A coupon (38 cm x 38 cm) was fabricated and tested which demonstrated an array specific power level of 60 W/kg with a potential of reaching 80 W/kg. The second panel design involved the use of newly developed high efficiency (22%) dual junction GaInP2/GaAs/Ge solar cells combined with an advanced lightweight rigid substrate using aluminum honeycomb core with high strength graphite fiber mesh facesheets. A coupon (38 cm x 38 cm) was fabricated and tested which demonstrated an array specific power of 105 W/kg and 230 W/sq m. This paper will address the construction details of the panels and an a analysis of the component weights. A strawman array design suitable for a typical small-sat mission is described for each of the two panel design technologies being studied. Benefits in respect to weight reduction, area reduction, and system cost reduction are analyzed and compared to conventional arrays.

Microbial Diagnostic Array Workstation (MDAW): a web server for diagnostic array data storage, sharing and analysis

PubMed Central

Scaria, Joy; Sreedharan, Aswathy; Chang, Yung-Fu

2008-01-01

Background Microarrays are becoming a very popular tool for microbial detection and diagnostics. Although these diagnostic arrays are much simpler when compared to the traditional transcriptome arrays, due to the high throughput nature of the arrays, the data analysis requirements still form a bottle neck for the widespread use of these diagnostic arrays. Hence we developed a new online data sharing and analysis environment customised for diagnostic arrays. Methods Microbial Diagnostic Array Workstation (MDAW) is a database driven application designed in MS Access and front end designed in ASP.NET. Conclusion MDAW is a new resource that is customised for the data analysis requirements for microbial diagnostic arrays. PMID:18811969

Microbial Diagnostic Array Workstation (MDAW): a web server for diagnostic array data storage, sharing and analysis.

PubMed

Scaria, Joy; Sreedharan, Aswathy; Chang, Yung-Fu

2008-09-23

Microarrays are becoming a very popular tool for microbial detection and diagnostics. Although these diagnostic arrays are much simpler when compared to the traditional transcriptome arrays, due to the high throughput nature of the arrays, the data analysis requirements still form a bottle neck for the widespread use of these diagnostic arrays. Hence we developed a new online data sharing and analysis environment customised for diagnostic arrays. Microbial Diagnostic Array Workstation (MDAW) is a database driven application designed in MS Access and front end designed in ASP.NET. MDAW is a new resource that is customised for the data analysis requirements for microbial diagnostic arrays.

Basic performance evaluation of a Si-PM array-based LGSO phoswich DOI block detector for a high-resolution small animal PET system.

PubMed

Yamamoto, Seiichi

2013-07-01

The silicon photomultiplier (Si-PM) is a promising photodetector for PET. However, it remains unclear whether Si-PM can be used for a depth-of-interaction (DOI) detector based on the decay time differences of the scintillator where pulse shape analysis is used. For clarification, we tested the Hamamatsu 4 × 4 Si-PM array (S11065-025P) combined with scintillators that used different decay times to develop DOI block detectors using the pulse shape analysis. First, Ce-doped Gd(2)SiO(5) (GSO) scintillators of 0.5 mol% Ce were arranged in a 4 × 4 matrix and were optically coupled to the center of each pixel of the Si-PM array for measurement of the energy resolution as well as its gain variations according to the temperature. Then two types of Ce-doped Lu(1.9)Gd(0.1)Si0(5) (LGSO) scintillators, 0.025 mol% Ce (decay time: ~31 ns) and 0.75 mol% Ce (decay time: ~46 ns), were optically coupled in the DOI direction, arranged in a 11 × 7 matrix, and optically coupled to a Si-PM array for testing of the possibility of a high-resolution DOI detector. The energy resolution of the Si-PM array-based GSO block detector was 18 ± 4.4 % FWHM for a Cs-137 gamma source (662 keV). Less than 1 mm crystals were clearly resolved in the position map of the LGSO DOI block detector. The peak-to-valley ratio (P/V) derived from the pulse shape spectra of the LGSO DOI block detector was 2.2. These results confirmed that Si-PM array-based DOI block detectors are promising for high-resolution small animal PET systems.

A Discovery Resource of Rare Copy Number Variations in Individuals with Autism Spectrum Disorder

PubMed Central

Prasad, Aparna; Merico, Daniele; Thiruvahindrapuram, Bhooma; Wei, John; Lionel, Anath C.; Sato, Daisuke; Rickaby, Jessica; Lu, Chao; Szatmari, Peter; Roberts, Wendy; Fernandez, Bridget A.; Marshall, Christian R.; Hatchwell, Eli; Eis, Peggy S.; Scherer, Stephen W.

2012-01-01

The identification of rare inherited and de novo copy number variations (CNVs) in human subjects has proven a productive approach to highlight risk genes for autism spectrum disorder (ASD). A variety of microarrays are available to detect CNVs, including single-nucleotide polymorphism (SNP) arrays and comparative genomic hybridization (CGH) arrays. Here, we examine a cohort of 696 unrelated ASD cases using a high-resolution one-million feature CGH microarray, the majority of which were previously genotyped with SNP arrays. Our objective was to discover new CNVs in ASD cases that were not detected by SNP microarray analysis and to delineate novel ASD risk loci via combined analysis of CGH and SNP array data sets on the ASD cohort and CGH data on an additional 1000 control samples. Of the 615 ASD cases analyzed on both SNP and CGH arrays, we found that 13,572 of 21,346 (64%) of the CNVs were exclusively detected by the CGH array. Several of the CGH-specific CNVs are rare in population frequency and impact previously reported ASD genes (e.g., NRXN1, GRM8, DPYD), as well as novel ASD candidate genes (e.g., CIB2, DAPP1, SAE1), and all were inherited except for a de novo CNV in the GPHN gene. A functional enrichment test of gene-sets in ASD cases over controls revealed nucleotide metabolism as a potential novel pathway involved in ASD, which includes several candidate genes for follow-up (e.g., DPYD, UPB1, UPP1, TYMP). Finally, this extensively phenotyped and genotyped ASD clinical cohort serves as an invaluable resource for the next step of genome sequencing for complete genetic variation detection. PMID:23275889

Flexible, phase-matched, linear receive arrays for high-field MRI in monkeys.

PubMed

Goense, Jozien; Logothetis, Nikos K; Merkle, Hellmut

2010-10-01

High signal-to-noise ratios (SNR) are essential for high-resolution anatomical and functional MRI. Phased arrays are advantageous for this but have the drawback that they often have inflexible and bulky configurations. Particularly in experiments where functional MRI is combined with simultaneous electrophysiology, space constraints can be prohibitive. To this end we developed a highly flexible multiple receive element phased array for use on anesthetized monkeys. The elements are interchangeable and different sizes and combinations of coil elements can be used, for instance, combinations of single and overlapped elements. The preamplifiers including control electronics are detachable and can serve a variety of prefabricated and phase matched arrays of different configurations, allowing the elements to always be placed in close proximity to the area of interest. Optimizing performance of the individual elements ensured high SNR at the cortical surface as well as in deeper laying structures. Performance of a variety of arrangements of gapped linear arrays was evaluated at 4.7 and 7T in high-resolution anatomical and functional MRI. Copyright © 2010 Elsevier Inc. All rights reserved.

Corrosion sensor

DOEpatents

Glass, Robert S.; Clarke, Jr., Willis L.; Ciarlo, Dino R.

1994-01-01

A corrosion sensor array incorporating individual elements for measuring various elements and ions, such as chloride, sulfide, copper, hydrogen (pH), etc. and elements for evaluating the instantaneous corrosion properties of structural materials. The exact combination and number of elements measured or monitored would depend upon the environmental conditions and materials used which are subject to corrosive effects. Such a corrosion monitoring system embedded in or mounted on a structure exposed to the environment would serve as an early warning system for the onset of severe corrosion problems for the structure, thus providing a safety factor as well as economic factors. The sensor array is accessed to an electronics/computational system, which provides a means for data collection and analysis.

Corrosion sensor

DOEpatents

Glass, R.S.; Clarke, W.L. Jr.; Ciarlo, D.R.

1994-04-26

A corrosion sensor array is described incorporating individual elements for measuring various elements and ions, such as chloride, sulfide, copper, hydrogen (pH), etc. and elements for evaluating the instantaneous corrosion properties of structural materials. The exact combination and number of elements measured or monitored would depend upon the environmental conditions and materials used which are subject to corrosive effects. Such a corrosion monitoring system embedded in or mounted on a structure exposed to the environment would serve as an early warning system for the onset of severe corrosion problems for the structure, thus providing a safety factor as well as economic factors. The sensor array is accessed to an electronics/computational system, which provides a means for data collection and analysis. 7 figures.

Method for monitoring environmental and corrosion

DOEpatents

Glass, Robert S.; Clarke, Jr., Willis L.; Ciarlo, Dino R.

1995-01-01

A corrosion sensor array incorporating individual elements for measuring various elements and ions, such as chloride, sulfide, copper, hydrogen (pH), etc. and elements for evaluating the instantaneous corrosion properties of structural materials. The exact combination and number of elements measured or monitored would depend upon the environmental conditions and materials used which are subject to corrosive effects. Such a corrosion monitoring system embedded in or mounted on a structure exposed to the environment would serve as an early warning system for the onset of severe corrosion problems for the structure, thus providing a safety factor as well as economic factors. The sensor array is accessed to an electronics/computational system, which provides a means for data collection and analysis.

Peptide array-based interaction assay of solid-bound peptides and anchorage-dependant cells and its effectiveness in cell-adhesive peptide design.

PubMed

Kato, Ryuji; Kaga, Chiaki; Kunimatsu, Mitoshi; Kobayashi, Takeshi; Honda, Hiroyuki

2006-06-01

Peptide array, the designable peptide library covalently synthesized on cellulose support, was applied to assay peptide-cell interaction, between solid-bound peptides and anchorage-dependant cells, to study objective peptide design. As a model case, cell-adhesive peptides that could enhance cell growth as tissue engineering scaffold material, was studied. On the peptide array, the relative cell-adhesion ratio of NIH/3T3 cells was 2.5-fold higher on the RGDS (Arg-Gly-Asp-Ser) peptide spot as compared to the spot with no peptide, thus indicating integrin-mediated peptide-cell interaction. Such strong cell adhesion mediated by the RGDS peptide was easily disrupted by single residue substitution on the peptide array, thus indicating that the sequence recognition accuracy of cells was strictly conserved in our optimized scheme. The observed cellular morphological extension with active actin stress-fiber on the RGD motif-containing peptide supported our strategy that peptide array-based interaction assay of solid-bound peptide and anchorage-dependant cells (PIASPAC) could provide quantitative data on biological peptide-cell interaction. The analysis of 180 peptides obtained from fibronectin type III domain (no. 1447-1629) yielded 18 novel cell-adhesive peptides without the RGD motif. Taken together with the novel candidates, representative rules of ineffective amino acid usage were obtained from non-effective candidate sequences for the effective designing of cell-adhesive peptides. On comparing the amino acid usage of the top 20 and last 20 peptides from the 180 peptides, the following four brief design rules were indicated: (i) Arg or Lys of positively charged amino acids (except His) could enhance cell adhesion, (ii) small hydrophilic amino acids are favored in cell-adhesion peptides, (iii) negatively charged amino acids and small amino acids (except Gly) could reduce cell adhesion, and (iv) Cys and Met could be excluded from the sequence combination since they have less influence on the peptide design. Such rules that are indicative of the nature of the functional peptide sequence can be obtained only by the mass comparison analysis of PIASPAC using peptide array. By following such indicative rules, numerous amino acid combinations can be effectively screened for further examination of novel peptide design.

Locating sources within a dense sensor array using graph clustering

NASA Astrophysics Data System (ADS)

Gerstoft, P.; Riahi, N.

2017-12-01

We develop a model-free technique to identify weak sources within dense sensor arrays using graph clustering. No knowledge about the propagation medium is needed except that signal strengths decay to insignificant levels within a scale that is shorter than the aperture. We then reinterpret the spatial coherence matrix of a wave field as a matrix whose support is a connectivity matrix of a graph with sensors as vertices. In a dense network, well-separated sources induce clusters in this graph. The geographic spread of these clusters can serve to localize the sources. The support of the covariance matrix is estimated from limited-time data using a hypothesis test with a robust phase-only coherence test statistic combined with a physical distance criterion. The latter criterion ensures graph sparsity and thus prevents clusters from forming by chance. We verify the approach and quantify its reliability on a simulated dataset. The method is then applied to data from a dense 5200 element geophone array that blanketed of the city of Long Beach (CA). The analysis exposes a helicopter traversing the array and oil production facilities.

The design and development of an automatic control system for the in-duct cancellation of spinning modes of sound. M.S. Thesis

NASA Technical Reports Server (NTRS)

Harrington, W. W.

1973-01-01

The reduction is discussed of the discrete tones generated by jet engines which is essential for jet aircraft to meet present and proposed noise standards. The discrete tones generated by the blades and vanes propagate in the inlet and exhaust duct in the form of spiraling acoustic waves, or spinning modes. The reduction of these spinning modes by the cancellation effect of the combination of two acoustic fields was investigated. The spinning mode synthesizer provided the means for effective study of this noise reduction scheme. Two sets of electrical-acoustical transducers located in an equally-spaced circular array simultaneously generate a specified spinning mode and the cancelling mode. Analysis of the wave equation for the synthesizer established the optimum cancelling array acoustic parameters for maximum sound pressure level reduction. The parameter dependence of the frequency ranges of propagation of single, specified circumferential modes generated by a single array, and of effective cancellation of the modes generated by two arrays, was determined. Substantial sound pressure level reduction was obtained for modes within these limits.

Development and characterization of a multi-layer magnetorheological elastomer isolator based on a Halbach array

NASA Astrophysics Data System (ADS)

Przybylski, Michal; Sun, Shuaishuai; Li, Weihua

2016-10-01

Most existing vibration isolators and dampers based on magnetorheological (MR) materials need electrical power to feed magnetic coils to stimulate the MR material, so if there is a loss of power, such as during a strong earthquake or system failure, they are unable to protect the structure. This paper outlines the design and test of a controllable multilayered magnetorheological elastomer (MRE) isolator based on a circular dipolar Halbach array; which is a set of magnets that generates a strong and uniform magnetic field. Combining an MRE layered isolator system with the Halbach array allows for constant vibration isolation with very low power consumption, where the power generated is only used to adjust the Halbach position. When this system was tested it successfully altered the lateral stiffness and damping force by 81.13% and 148.72%, respectively. This paper also includes an extended analysis of the magnetic field generated by the circular dipolar Halbach array and a discussion of the improvements that may potentially improve the range of magnetic fields generated.

Synthesis of ultrawideband radiation of combined antenna arrays excited by nanosecond bipolar voltage pulses

NASA Astrophysics Data System (ADS)

Koshelev, V. I.; Plisko, V. V.; Sevostyanov, E. A.

2017-05-01

To broaden the spectrum of high-power ultrawideband radiation, it is suggested to synthesize an electromagnetic pulse summing the pulses of different length in free space. On the example of model pulses corresponding to radiation of combined antennas excited by bipolar voltage pulses of the length of 2 and 3 ns, the possibility of twofold broadening of the radiation spectrum was demonstrated. Radiation pulses with the spectrum width exceeding three octaves were obtained. Pattern formation by the arrays of different geometry excited by the pulses having different time shifts was considered. Optimum array structure with the pattern maximum in the main direction was demonstrated on the example of a 2×2 array.

NEW LENSLET BASED IFS WITH HIGH DETECTOR PIXEL EFFICIENCY

NASA Astrophysics Data System (ADS)

Gong, Qian

2018-01-01

Three IFS types currently used for optical design are: lenslet array, imager slicer, and lenslet array and fiber combined. Lenslet array based Integral Field Spectroscopy (IFS) is very popular for many astrophysics applications due to its compactness, simplicity, as well as cost and mass savings. The disadvantage of lenslet based IFS is its low detector pixel efficiency. Enough spacing is needed between adjacent spectral traces in cross dispersion direction to avoid wavelength cross-talk, because the same wavelength is not aligned to the same column on detector. Such as on a recent exoplanet coronagraph instrument study to support the coming astrophysics decadal survey (LUVOIR), to cover a 45 λ/D Field of View (FOV) with a spectral resolving power of 200 at shortest wavelength, a 4k x 4k detector array is needed. This large format EMCCD pushes the detector into technology development area with a low TRL. Besides the future mission, it will help WFIRST coronagraph IFS by packing all spectra into a smaller area on detector, which will reduce the chance for electrons to be trapped in pixels, and slow the detector degradation during the mission.The innovation we propose here is to increase the detector packing efficiency by grouping a number of lenslets together to form many mini slits. In other words, a number of spots (Point Spread Function at lenslet focus) are aligned into a line to resemble a mini slit. Therefore, wavelength cross-talk is no longer a concern anymore. This combines the advantage of lenslet array and imager slicer together. The isolation rows between spectral traces in cross dispersion direction can be reduced or removed. So the packing efficiency is greatly increased. Furthermore, the today’s microlithography and etching technique is capable of making such a lenslet array, which will relax the detector demand significantly. It will finally contribute to the habitable exoplanets study to analyzing their spectra from direct images. Detailed theory, design, analysis, and fabrication status will be presented.

Analysis of seismic waves crossing the Santa Clara Valley using the three-component MUSIQUE array algorithm

NASA Astrophysics Data System (ADS)

Hobiger, Manuel; Cornou, Cécile; Bard, Pierre-Yves; Le Bihan, Nicolas; Imperatori, Walter

2016-10-01

We introduce the MUSIQUE algorithm and apply it to seismic wavefield recordings in California. The algorithm is designed to analyse seismic signals recorded by arrays of three-component seismic sensors. It is based on the MUSIC and the quaternion-MUSIC algorithms. In a first step, the MUSIC algorithm is applied in order to estimate the backazimuth and velocity of incident seismic waves and to discriminate between Love and possible Rayleigh waves. In a second step, the polarization parameters of possible Rayleigh waves are analysed using quaternion-MUSIC, distinguishing retrograde and prograde Rayleigh waves and determining their ellipticity. In this study, we apply the MUSIQUE algorithm to seismic wavefield recordings of the San Jose Dense Seismic Array. This array has been installed in 1999 in the Evergreen Basin, a sedimentary basin in the Eastern Santa Clara Valley. The analysis includes 22 regional earthquakes with epicentres between 40 and 600 km distant from the array and covering different backazimuths with respect to the array. The azimuthal distribution and the energy partition of the different surface wave types are analysed. Love waves dominate the wavefield for the vast majority of the events. For close events in the north, the wavefield is dominated by the first harmonic mode of Love waves, for farther events, the fundamental mode dominates. The energy distribution is different for earthquakes occurring northwest and southeast of the array. In both cases, the waves crossing the array are mostly arriving from the respective hemicycle. However, scattered Love waves arriving from the south can be seen for all earthquakes. Combining the information of all events, it is possible to retrieve the Love wave dispersion curves of the fundamental and the first harmonic mode. The particle motion of the fundamental mode of Rayleigh waves is retrograde and for the first harmonic mode, it is prograde. For both modes, we can also retrieve dispersion and ellipticity curves. Wave motion simulations for two earthquakes are in good agreement with the real data results and confirm the identification of the wave scattering formations to the south of the array, which generate the scattered Love waves visible for all earthquakes.

Seismo-acoustic analysis of the near quarry blasts using Plostina small aperture array

NASA Astrophysics Data System (ADS)

Ghica, Daniela; Stancu, Iulian; Ionescu, Constantin

2013-04-01

Seismic and acoustic signals are important to recognize different type of industrial blasting sources in order to discriminate between them and natural earthquakes. We have analyzed the seismic events listed in the Romanian catalogue (Romplus) for the time interval between 2011 and 2012, and occurred in the Dobrogea region, in order to determine detection seismo-acoustic signals of quarry blasts by Plostina array stations. Dobrogea is known as a seismic region characterized by crustal earthquakes with low magnitudes; at the same time, over 40 quarry mines are located in the area, being sources of blasts recorded both with the seismic and infrasound sensors of the Romanian Seismic Network. Plostina seismo-acoustic array, deployed in the central part of Romania, consists of 7 seismic sites (3C broad-band instruments and accelerometers) collocated with 7 infrasound instruments. The array is particularly used for the seismic monitoring of the local and regional events, as well as for the detection of infrasonic signals produced by various sources. Considering the characteristics of the infrasound sensors (frequency range, dynamic, sensibility), the array proved its efficiency in observing the signals produced by explosions, mine explosion and quarry blasts. The quarry mines included for this study cover distances of two hundreds of kilometers from the station and routinely generate explosions that are detected as seismic and infrasonic signals with Plostina array. The combined seismo-acoustic analysis uses two types of detectors for signal identification: one, applied for the seismic signal identification, is based on array processing techniques (beamforming and frequency-wave number analysis), while the other one, which is used for infrasound detection and characterization, is the automatic detector DFX-PMCC (Progressive Multi-Channel Correlation Method). Infrasonic waves generated by quarry blasts have frequencies ranging from 0.05 Hz up to at least 6 Hz and amplitudes below 5 Pa. Seismic data analysis shows that the frequency range of the signals are above 2 Hz. Surface explosions such as quarry blasts are useful sources for checking detection and location efficiency, when seismic measurements are added. The process is crucial for discrimination purposes and for establishing of a set of ground-truth infrasound events. Ground truth information plays a key role in the interpretation of infrasound signals, by including near-field observations from industrial blasts.

Channel capacity of an array system for Gaussian channels with applications to combining and noise cancellation

NASA Technical Reports Server (NTRS)

Cheung, K. M.; Vilnrotter, V.

1996-01-01

A closed-form expression for the capacity of an array of correlated Gaussian channels is derived. It is shown that when signal and noise are independent, the array of observables can be replaced with a single observable without diminishing the capacity of the array channel. Examples are provided to illustrate the dependence of channel capacity on noise correlation for two- and three-channel arrays.

Channel Capacity of an Array System for Gaussian Channels With Applications to Combining and Noise Cancellation

NASA Technical Reports Server (NTRS)

Cheung, K.-M.; Vilnrotter, V.

1996-01-01

A closed-form expression for the capacity of an array of correlated Gaussian channels is derived. It is shown that when signal and noise are independent, the array of observables can be replaced with a single observable without diminishing the capacity of the array channel. Examples are provided to illustrate the dependence of channel capacity on noise correlation for two- and three-channel arrays.

Combining module based on coherent polarization beam combining.

PubMed

Yang, Yan; Geng, Chao; Li, Feng; Li, Xinyang

2017-03-01

A multiaperture receiver with a phased array is an effective approach to overcome the effect of the random optical disturbance in coherent free-space laser communications, in which one of the key technologies is how to efficiently combine the multiple laser beams received by the phased array antenna. A combining module based on coherent polarization beam combining (CPBC), which can combine multiple laser beams to one laser beam with high combining efficiency and output a linearly polarized beam, is proposed in this paper. The principle of the combining module is introduced, the coherent polarization combining efficiency of CPBC is analyzed, and the performance of the combining module is evaluated. Moreover, the feasibility and the expansibility of the proposed combining module are validated in experiments of CPBC based on active phase-locking.

Development of a General-Purpose Analysis System Based on a Programmable Fluid Processor Final Report CRADA No. TC-2027-01

DOE Office of Scientific and Technical Information (OSTI.GOV)

McConaghy, C. F.; Gascoyne, P. R.

The purpose ofthis project was to develop a general-purpose analysis system based on a programmable fluid processor (PFP). The PFP is an array of electrodes surrounded by fluid reservoirs and injectors. Injected droplets of various reagents are manjpulated and combined on the array by Dielectrophoretic (DEP) forces. The goal was to create a small handheld device that could accomplish the tasks currently undertaken by much larger, time consuming, manual manipulation in the lab. The entire effo1t was funded by DARPA under the Bio-Flips program. MD Anderson Cancer Center was the PI for the DARPA effort. The Bio-Flips program was amore » 3- year program that ran from September 2000 to September 2003. The CRADA was somewhat behind the Bi-Flips program running from June 2001 to June 2004 with a no cost extension to September 2004.« less

Semiconductor nanomembrane-based sensors for high frequency pressure measurements

NASA Astrophysics Data System (ADS)

Ruan, Hang; Kang, Yuhong; Homer, Michelle; Claus, Richard O.; Mayo, David; Sibold, Ridge; Jones, Tyler; Ng, Wing

2017-04-01

This paper demonstrates improvements on semiconductor nanomembrane based high frequency pressure sensors that utilize silicon on insulator techniques in combination with nanocomposite materials. The low-modulus, conformal nanomembrane sensor skins with integrated interconnect elements and electronic devices could be applied to vehicles or wind tunnel models for full spectrum pressure analysis. Experimental data demonstrates that: 1) silicon nanomembrane may be used as single pressure sensor transducers and elements in sensor arrays, 2) the arrays may be instrumented to map pressure over the surfaces of test articles over a range of Reynolds numbers, temperature and other environmental conditions, 3) in the high frequency range, the sensor is comparable to the commercial high frequency sensor, and 4) in the low frequency range, the sensor is much better than the commercial sensor. This supports the claim that nanomembrane pressure sensors may be used for wide bandwidth flow analysis.

Exome Array Analysis Identifies a Common Variant in IL27 Associated with Chronic Obstructive Pulmonary Disease

PubMed Central

Parker, Margaret M.; Chen, Han; Lao, Taotao; Hardin, Megan; Qiao, Dandi; Hawrylkiewicz, Iwona; Sliwinski, Pawel; Yim, Jae-Joon; Kim, Woo Jin; Kim, Deog Kyeom; Castaldi, Peter J.; Hersh, Craig P.; Morrow, Jarrett; Celli, Bartolome R.; Pinto-Plata, Victor M.; Criner, Gerald J.; Marchetti, Nathaniel; Bueno, Raphael; Agustí, Alvar; Make, Barry J.; Crapo, James D.; Calverley, Peter M.; Donner, Claudio F.; Lomas, David A.; Wouters, Emiel F. M.; Vestbo, Jorgen; Paré, Peter D.; Levy, Robert D.; Rennard, Stephen I.; Zhou, Xiaobo; Laird, Nan M.; Lin, Xihong; Beaty, Terri H.; Silverman, Edwin K.

2016-01-01

Rationale: Chronic obstructive pulmonary disease (COPD) susceptibility is in part related to genetic variants. Most genetic studies have been focused on genome-wide common variants without a specific focus on coding variants, but common and rare coding variants may also affect COPD susceptibility. Objectives: To identify coding variants associated with COPD. Methods: We tested nonsynonymous, splice, and stop variants derived from the Illumina HumanExome array for association with COPD in five study populations enriched for COPD. We evaluated single variants with a minor allele frequency greater than 0.5% using logistic regression. Results were combined using a fixed effects meta-analysis. We replicated novel single-variant associations in three additional COPD cohorts. Measurements and Main Results: We included 6,004 control subjects and 6,161 COPD cases across five cohorts for analysis. Our top result was rs16969968 (P = 1.7 × 10−14) in CHRNA5, a locus previously associated with COPD susceptibility and nicotine dependence. Additional top results were found in AGER, MMP3, and SERPINA1. A nonsynonymous variant, rs181206, in IL27 (P = 4.7 × 10−6) was just below the level of exome-wide significance but attained exome-wide significance (P = 5.7 × 10−8) when combined with results from other cohorts. Gene expression datasets revealed an association of rs181206 and the surrounding locus with expression of multiple genes; several were differentially expressed in COPD lung tissue, including TUFM. Conclusions: In an exome array analysis of COPD, we identified nonsynonymous variants at previously described loci and a novel exome-wide significant variant in IL27. This variant is at a locus previously described in genome-wide associations with diabetes, inflammatory bowel disease, and obesity and appears to affect genes potentially related to COPD pathogenesis. PMID:26771213

Performance and characterization of new micromachined high-frequency linear arrays.

PubMed

Lukacs, Marc; Yin, Jianhua; Pang, Guofeng; Garcia, Richard C; Cherin, Emmanuel; Williams, Ross; Mehi, Jim; Foster, F Stuart

2006-10-01

A new approach for fabricating high frequency (> 20 MHz) linear array transducers, based on laser micromachining, has been developed. A 30 MHz, 64-element, 74-microm pitch, linear array design is presented. The performance of the device is demonstrated by comparing electrical and acoustic measurements with analytical, equivalent circuit, and finite-element analysis (FEA) simulations. All FEA results for array performance have been generated using one global set of material parameters. Each fabricated array has been integrated onto a flex circuit for ease of handling, and the flex has been integrated onto a custom printed circuit board test card for ease of testing. For a fully assembled array, with an acoustic lens, the center frequency was 28.7 MHz with a one-way -3 dB and -6 dB bandwidth of 59% and 83%, respectively, and a -20 dB pulse width of -99 ns. The per-element peak acoustic power, for a +/- 30 V single cycle pulse, measured at the 10 mm focal length of the lens was 590 kPa with a -6 dB directivity span of about 30 degrees. The worst-case total cross talk of the combined array and flex assembly is for nearest neighboring elements and was measured to have an average level -40 dB across the -6 dB bandwidth of the device. Any significant deviation from simulation can be explained through limitations in apparatus calibration and in device packaging.

Proteomic analysis of protein deposits on worn daily wear silicone hydrogel contact lenses

PubMed Central

Wei, Xiaojia; Aliwarga, Yulina; Carnt, Nicole A.; Garrett, Qian; Willcox, Mark D.P.

2008-01-01

Purpose Previous studies have demonstrated deposition of tear proteins onto worn contact lenses. In this study, we used proteomic techniques to analyze the protein deposits extracted from worn daily wear silicone hydrogel contact lenses in combination with different lens care solutions. Methods Worn lenses were collected and protein deposits extracted using urea and surfactant. Protein extracts were desalted, concentrated, and then separated using one-dimensional gel electrophoresis. Individual protein components in extracts were identified using liquid chromatography combined with tandem mass spectrometry (LC-MS-MS) after trypsin digestion. Results One-dimensional gel electrophoresis revealed that lysozyme and other small proteins (around 20 kDa) were the most abundant proteins in the extracts. LC-MS-MS revealed a wide array of proteins in lens extracts with lysozyme and lipocalin 1 being the most commonly identified in deposit extracts. Conclusions Worn contact lenses deposit a wide array of proteins from tear film and other sources. Protein deposit profiles varied and were specific for each contact lens material. PMID:18989384

Transcriptional analysis of product-concentration driven changes in cellular programs of recombinant Clostridium acetobutylicumstrains.

PubMed

Tummala, Seshu B; Junne, Stefan G; Paredes, Carlos J; Papoutsakis, Eleftherios T

2003-12-30

Antisense RNA (asRNA) downregulation alters protein expression without changing the regulation of gene expression. Downregulation of primary metabolic enzymes possibly combined with overexpression of other metabolic enzymes may result in profound changes in product formation, and this may alter the large-scale transcriptional program of the cells. DNA-array based large-scale transcriptional analysis has the potential to elucidate factors that control cellular fluxes even in the absence of proteome data. These themes are explored in the study of large-scale transcriptional analysis programs and the in vivo primary-metabolism fluxes of several related recombinant C. acetobutylicum strains: C. acetobutylicum ATCC 824(pSOS95del) (plasmid control; produces high levels of butanol snd acetone), 824(pCTFB1AS) (expresses antisense RNA against CoA transferase (ctfb1-asRNA); produces very low levels of butanol and acetone), and 824(pAADB1) (expresses ctfb1-asRNA and the alcohol-aldehyde dahydrogenase gene (aad); produce high alcohol and low acetone levels). DNA-array based transcriptional analysis revealed that the large changes in product concentrations (snd notably butanol concentration) due to ctfb1-asRNA expression alone and in combination with aad overexpression resulted in dramatic changes of the cellular transcriptome. Cluster analysis and gene expression patterns of established and putative operons involved in stress response, motility, sporulation, and fatty-acid biosynthesis indicate that these simple genetic changes dramatically alter the cellular programs of C. acetobutylicum. Comparison of gene expression and flux analysis data may point to possible flux-controling steps and suggest unknown regulatory mechanisms. Copyright 2003; Wiley Periodicals, Inc.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Steele, Amy; Hughes, A. Meredith; Carpenter, John

The presence of debris disks around young main-sequence stars hints at the existence and structure of planetary systems. Millimeter-wavelength observations probe large grains that trace the location of planetesimal belts. The Formation and Evolution of Planetary Systems Spitzer Legacy survey of nearby young solar analogues yielded a sample of five debris disk-hosting stars with millimeter flux suitable for interferometric follow-up. We present observations with the Submillimeter Array (SMA) and the Combined Array for Research in Millimeter-wave Astronomy at ∼2″ resolution that spatially resolve the debris disks around these nearby (d ∼ 50 pc) stars. Two of the five disks (HDmore » 377, HD 8907) are spatially resolved for the first time and one (HD 104860) is resolved at millimeter wavelengths for the first time. We combine our new observations with archival SMA and Atacama Large Millimeter/Submillimeter Array data to enable a uniform analysis of the full five-object sample. We simultaneously model the broadband photometric data and resolved millimeter visibilities to constrain the dust temperatures and disk morphologies, and perform a Markov Chain Monte Carlo analysis to fit for basic structural parameters. We find that the radii and widths of the cold outer belts exhibit properties consistent with scaled-up versions of the Solar System's Kuiper Belt. All the disks exhibit characteristic grain sizes comparable to the blowout size, and all the resolved observations of emission from large dust grains are consistent with an axisymmetric dust distribution to within the uncertainties. These results are consistent with comparable studies carried out at infrared wavelengths.« less

PhylArray: phylogenetic probe design algorithm for microarray.

PubMed

Militon, Cécile; Rimour, Sébastien; Missaoui, Mohieddine; Biderre, Corinne; Barra, Vincent; Hill, David; Moné, Anne; Gagne, Geneviève; Meier, Harald; Peyretaillade, Eric; Peyret, Pierre

2007-10-01

Microbial diversity is still largely unknown in most environments, such as soils. In order to get access to this microbial 'black-box', the development of powerful tools such as microarrays are necessary. However, the reliability of this approach relies on probe efficiency, in particular sensitivity, specificity and explorative power, in order to obtain an image of the microbial communities that is close to reality. We propose a new probe design algorithm that is able to select microarray probes targeting SSU rRNA at any phylogenetic level. This original approach, implemented in a program called 'PhylArray', designs a combination of degenerate and non-degenerate probes for each target taxon. Comparative experimental evaluations indicate that probes designed with PhylArray yield a higher sensitivity and specificity than those designed by conventional approaches. Applying the combined PhyArray/GoArrays strategy helps to optimize the hybridization performance of short probes. Finally, hybridizations with environmental targets have shown that the use of the PhylArray strategy can draw attention to even previously unknown bacteria.

Antenna array geometry optimization for a passive coherent localisation system

NASA Astrophysics Data System (ADS)

Knott, Peter; Kuschel, Heiner; O'Hagan, Daniel

2012-11-01

Passive Coherent Localisation (PCL), also known as Passive Radar, making use of RF sources of opportunity such as Radio or TV Broadcasting Stations, Cellular Phone Network Base Stations, etc. is an advancing technology for covert operation because no active radar transmitter is required. It is also an attractive addition to existing active radar stations because it has the potential to discover low-flying and low-observable targets. The CORA (Covert Radar) experimental passive radar system currently developed at Fraunhofer-FHR features a multi-channel digital radar receiver and a circular antenna array with separate elements for the VHF- and the UHF-range and is used to exploit alternatively Digital Audio (DAB) or Video Broadcasting (DVB-T) signals. For an extension of the system, a wideband antenna array is being designed for which a new discone antenna element has been developed covering the full DVB-T frequency range. The present paper describes the outline of the system and the numerical modelling and optimisation methods applied to solve the complex task of antenna array design: Electromagnetic full wave analysis is required for the parametric design of the antenna elements while combinatorial optimization methods are applied to find the best array positions and excitation coefficients for a regular omni-directional antenna performance. The different steps are combined in an iterative loop until the optimum array layout is found. Simulation and experimental results for the current system will be shown.

Radio detection of high-energy cosmic rays with the Auger Engineering Radio Array

NASA Astrophysics Data System (ADS)

Schröder, Frank G.; Pierre Auger Collaboration

2016-07-01

The Auger Engineering Radio Array (AERA) is an enhancement of the Pierre Auger Observatory in Argentina. Covering about 17km2, AERA is the world-largest antenna array for cosmic-ray observation. It consists of more than 150 antenna stations detecting the radio signal emitted by air showers, i.e., cascades of secondary particles caused by primary cosmic rays hitting the atmosphere. At the beginning, technical goals had been in focus: first of all, the successful demonstration that a large-scale antenna array consisting of autonomous stations is feasible. Moreover, techniques for calibration of the antennas and time calibration of the array have been developed, as well as special software for the data analysis. Meanwhile physics goals come into focus. At the Pierre Auger Observatory air showers are simultaneously detected by several detector systems, in particular water-Cherenkov detectors at the surface, underground muon detectors, and fluorescence telescopes, which enables cross-calibration of different detection techniques. For the direction and energy of air showers, the precision achieved by AERA is already competitive; for the type of primary particle, several methods are tested and optimized. By combining AERA with the particle detectors we aim for a better understanding of cosmic rays in the energy range from approximately 0.3 to 10 EeV, i.e., significantly higher energies than preceding radio arrays.

Micro-Analyzer: automatic preprocessing of Affymetrix microarray data.

PubMed

Guzzi, Pietro Hiram; Cannataro, Mario

2013-08-01

A current trend in genomics is the investigation of the cell mechanism using different technologies, in order to explain the relationship among genes, molecular processes and diseases. For instance, the combined use of gene-expression arrays and genomic arrays has been demonstrated as an effective instrument in clinical practice. Consequently, in a single experiment different kind of microarrays may be used, resulting in the production of different types of binary data (images and textual raw data). The analysis of microarray data requires an initial preprocessing phase, that makes raw data suitable for use on existing analysis platforms, such as the TIGR M4 (TM4) Suite. An additional challenge to be faced by emerging data analysis platforms is the ability to treat in a combined way those different microarray formats coupled with clinical data. In fact, resulting integrated data may include both numerical and symbolic data (e.g. gene expression and SNPs regarding molecular data), as well as temporal data (e.g. the response to a drug, time to progression and survival rate), regarding clinical data. Raw data preprocessing is a crucial step in analysis but is often performed in a manual and error prone way using different software tools. Thus novel, platform independent, and possibly open source tools enabling the semi-automatic preprocessing and annotation of different microarray data are needed. The paper presents Micro-Analyzer (Microarray Analyzer), a cross-platform tool for the automatic normalization, summarization and annotation of Affymetrix gene expression and SNP binary data. It represents the evolution of the μ-CS tool, extending the preprocessing to SNP arrays that were not allowed in μ-CS. The Micro-Analyzer is provided as a Java standalone tool and enables users to read, preprocess and analyse binary microarray data (gene expression and SNPs) by invoking TM4 platform. It avoids: (i) the manual invocation of external tools (e.g. the Affymetrix Power Tools), (ii) the manual loading of preprocessing libraries, and (iii) the management of intermediate files, such as results and metadata. Micro-Analyzer users can directly manage Affymetrix binary data without worrying about locating and invoking the proper preprocessing tools and chip-specific libraries. Moreover, users of the Micro-Analyzer tool can load the preprocessed data directly into the well-known TM4 platform, extending in such a way also the TM4 capabilities. Consequently, Micro Analyzer offers the following advantages: (i) it reduces possible errors in the preprocessing and further analysis phases, e.g. due to the incorrect choice of parameters or due to the use of old libraries, (ii) it enables the combined and centralized pre-processing of different arrays, (iii) it may enhance the quality of further analysis by storing the workflow, i.e. information about the preprocessing steps, and (iv) finally Micro-Analzyer is freely available as a standalone application at the project web site http://sourceforge.net/projects/microanalyzer/. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

In situ STM investigation of aromatic poly(azomethine) arrays constructed by "on-site" equilibrium polymerization.

PubMed

Tanoue, Ryota; Higuchi, Rintaro; Ikebe, Kiryu; Uemura, Shinobu; Kimizuka, Nobuo; Stieg, Adam Z; Gimzewski, James K; Kunitake, Masashi

2012-10-02

Two-dimensional (2D) arrays of π-conjugated aromatic polymers produced by surface-selective Schiff base coupling reactions between an aromatic diamine and an aromatic dialdehyde were investigated in detail using in situ scanning tunneling microscopy. Surface-selective coupling was achieved for almost all diamine/dialdehyde combinations attempted, although several combinations did not proceed even in homogeneous aqueous alkaline solution. Most of the combinations of an aromatic diamine and a dialdehyde, except the combinations of 4,4'-azodianiline with mono/bithiophenedicarboxaldehyde, formed highly ordered π-conjugated polymer arrays on an iodine-modified Au(111) surface in aqueous solution at a suitable pH. The simplest polymer of the various combinations tested, obtained from the combination of 1,4-diaminobenzene with terephthaldicarboxaldehyde, gave a 2D array consisting of linearly connected benzene units. Poly(azomethine) adlayers caused a positive shift in the electrochemical potential of the butterfly shaped oxidative adsorption and reductive desorption of iodine. The acceleration of the reductive desorption of iodine suggests the existence of a weak interaction between the polymer layer and iodine. Not only the first polymer adlayers but also partially adsorbed secondary adlayers with "on-top" epitaxial behavior were frequently observed for all polymer systems. The alignment of the polymer chains in the adlayers possessed a certain regularity in terms of a regular interval between polymer chains because of repulsive interpolymer interactions.

Meta-GWAS and Meta-Analysis of Exome Array Studies Do Not Reveal Genetic Determinants of Serum Hepcidin

PubMed Central

Galesloot, Tessel E.; van Dijk, Freerk; Geurts-Moespot, Anneke J.; Girelli, Domenico; Kiemeney, Lambertus A. L. M.; Sweep, Fred C. G. J.; Swertz, Morris A.; van der Meer, Peter; Camaschella, Clara; Toniolo, Daniela; Vermeulen, Sita H.; van der Harst, Pim; Swinkels, Dorine W.

2016-01-01

Serum hepcidin concentration is regulated by iron status, inflammation, erythropoiesis and numerous other factors, but underlying processes are incompletely understood. We studied the association of common and rare single nucleotide variants (SNVs) with serum hepcidin in one Italian study and two large Dutch population-based studies. We genotyped common SNVs with genome-wide association study (GWAS) arrays and subsequently performed imputation using the 1000 Genomes reference panel. Cohort-specific GWAS were performed for log-transformed serum hepcidin, adjusted for age and gender, and results were combined in a fixed-effects meta-analysis (total N 6,096). Six top SNVs (p<5x10-6) were genotyped in 3,821 additional samples, but associations were not replicated. Furthermore, we meta-analyzed cohort-specific exome array association results of rare SNVs with serum hepcidin that were available for two of the three cohorts (total N 3,226), but no exome-wide significant signal (p<1.4x10-6) was identified. Gene-based meta-analyses revealed 19 genes that showed significant association with hepcidin. Our results suggest the absence of common SNVs and rare exonic SNVs explaining a large proportion of phenotypic variation in serum hepcidin. We recommend extension of our study once additional substantial cohorts with hepcidin measurements, GWAS and/or exome array data become available in order to increase power to identify variants that explain a smaller proportion of hepcidin variation. In addition, we encourage follow-up of the potentially interesting genes that resulted from the gene-based analysis of low-frequency and rare variants. PMID:27846281

Design and integration of a generic disposable array-compatible sensor housing into an integrated disposable indirect microfluidic flow injection analysis system.

PubMed

Rapp, Bastian E; Schickling, Benjamin; Prokop, Jürgen; Piotter, Volker; Rapp, Michael; Länge, Kerstin

2011-10-01

We describe an integration strategy for arbitrary sensors intended to be used as biosensors in biomedical or bioanalytical applications. For such devices ease of handling (by a potential end user) as well as strict disposable usage are of importance. Firstly we describe a generic array compatible polymer sensor housing with an effective sample volume of 1.55 μl. This housing leaves the sensitive surface of the sensor accessible for the application of biosensing layers even after the embedding. In a second step we show how this sensor housing can be used in combination with a passive disposable microfluidic chip to set up arbitrary 8-fold sensor arrays and how such a system can be complemented with an indirect microfluidic flow injection analysis (FIA) system. This system is designed in a way that it strictly separates between disposable and reusable components- by introducing tetradecane as an intermediate liquid. This results in a sensor system compatible with the demands of most biomedical applications. Comparative measurements between a classical macroscopic FIA system and this integrated indirect microfluidic system are presented. We use a surface acoustic wave (SAW) sensor as an exemplary detector in this work.

Combined PET/MRI scanner

DOEpatents

Schlyer, David; Woody, Craig L.; Rooney, William; Vaska, Paul; Stoll, Sean; Pratte, Jean-Francois; O'Connor, Paul

2007-10-23

A combined PET/MRI scanner generally includes a magnet for producing a magnetic field suitable for magnetic resonance imaging, a radiofrequency (RF) coil disposed within the magnetic field produced by the magnet and a ring tomograph disposed within the magnetic field produced by the magnet. The ring tomograph includes a scintillator layer for outputting at least one photon in response to an annihilation event, a detection array coupled to the scintillator layer for detecting the at least one photon outputted by the scintillator layer and for outputting a detection signal in response to the detected photon and a front-end electronic array coupled to the detection array for receiving the detection signal, wherein the front-end array has a preamplifier and a shaper network for conditioning the detection signal.

Visual and Vestibular Determinants of Perceived Eye-Level

NASA Technical Reports Server (NTRS)

Cohen, Malcolm Martin

2003-01-01

Both gravitational and optical sources of stimulation combine to determine the perceived elevations of visual targets. The ways in which these sources of stimulation combine with one another in operational aeronautical environments are critical for pilots to make accurate judgments of the relative altitudes of other aircraft and of their own altitude relative to the terrain. In a recent study, my colleagues and I required eighteen observers to set visual targets at their apparent horizon while they experienced various levels of G(sub z) in the human centrifuge at NASA-Ames Research Center. The targets were viewed in darkness and also against specific background optical arrays that were oriented at various angles with respect to the vertical; target settings were lowered as Gz was increased; this effect was reduced when the background optical array was visible. Also, target settings were displaced in the direction that the background optical array was pitched. Our results were attributed to the combined influences of otolith-oculomotor mechanisms that underlie the elevator illusion and visual-oculomotor mechanisms (optostatic responses) that underlie the perceptual effects of viewing pitched optical arrays that comprise the background. In this paper, I present a mathematical model that describes the independent and combined effects of G(sub z) intensity and the orientation and structure of background optical arrays; the model predicts quantitative deviations from normal accurate perceptions of target localization under a variety of conditions. Our earlier experimental results and the mathematical model are described in some detail, and the effects of viewing specific optical arrays under various gravitational-inertial conditions encountered in aeronautical environments are discussed.

Array-based sensing using nanoparticles: an alternative approach for cancer diagnostics.

PubMed

Le, Ngoc D B; Yazdani, Mahdieh; Rotello, Vincent M

2014-07-01

Array-based sensing using nanoparticles (NPs) provides an attractive alternative to specific biomarker-focused strategies for cancer diagnosis. The physical and chemical properties of NPs provide both the recognition and transduction capabilities required for biosensing. Array-based sensors utilize a combined response from the interactions between sensors and analytes to generate a distinct pattern (fingerprint) for each analyte. These interactions can be the result of either the combination of multiple specific biomarker recognition (specific binding) or multiple selective binding responses, known as chemical nose sensing. The versatility of the latter array-based sensing using NPs can facilitate the development of new personalized diagnostic methodologies in cancer diagnostics, a necessary evolution in the current healthcare system to better provide personalized treatments. This review will describe the basic principle of array-based sensors, along with providing examples of both invasive and noninvasive samples used in cancer diagnosis.

Genome-wide common and rare variant analysis provides novel insights into clozapine-associated neutropenia.

PubMed

Legge, S E; Hamshere, M L; Ripke, S; Pardinas, A F; Goldstein, J I; Rees, E; Richards, A L; Leonenko, G; Jorskog, L F; Chambert, K D; Collier, D A; Genovese, G; Giegling, I; Holmans, P; Jonasdottir, A; Kirov, G; McCarroll, S A; MacCabe, J H; Mantripragada, K; Moran, J L; Neale, B M; Stefansson, H; Rujescu, D; Daly, M J; Sullivan, P F; Owen, M J; O'Donovan, M C; Walters, J T R

2017-10-01

The antipsychotic clozapine is uniquely effective in the management of schizophrenia; however, its use is limited by its potential to induce agranulocytosis. The causes of this, and of its precursor neutropenia, are largely unknown, although genetic factors have an important role. We sought risk alleles for clozapine-associated neutropenia in a sample of 66 cases and 5583 clozapine-treated controls, through a genome-wide association study (GWAS), imputed human leukocyte antigen (HLA) alleles, exome array and copy-number variation (CNV) analyses. We then combined associated variants in a meta-analysis with data from the Clozapine-Induced Agranulocytosis Consortium (up to 163 cases and 7970 controls). In the largest combined sample to date, we identified a novel association with rs149104283 (odds ratio (OR)=4.32, P=1.79 × 10 -8 ), intronic to transcripts of SLCO1B3 and SLCO1B7, members of a family of hepatic transporter genes previously implicated in adverse drug reactions including simvastatin-induced myopathy and docetaxel-induced neutropenia. Exome array analysis identified gene-wide associations of uncommon non-synonymous variants within UBAP2 and STARD9. We additionally provide independent replication of a previously identified variant in HLA-DQB1 (OR=15.6, P=0.015, positive predictive value=35.1%). These results implicate biological pathways through which clozapine may act to cause this serious adverse effect.

Genome-wide common and rare variant analysis provides novel insights into clozapine-associated neutropenia

PubMed Central

Legge, S E; Hamshere, M L; Ripke, S; Pardinas, A F; Goldstein, J I; Rees, E; Richards, A L; Leonenko, G; Jorskog, L F; Goldstein, Jacqueline I; Jarskog, L Fredrik; Hilliard, Chris; Alfirevic, Ana; Duncan, Laramie; Fourches, Denis; Huang, Hailiang; Lek, Monkol; Neale, Benjamin M; Ripke, Stephan; Shianna, Kevin; Szatkiewicz, Jin P; Tropsha, Alexander; van den Oord, Edwin JCG; Cascorbi, Ingolf; Dettling, Michael; Gazit, Ephraim; Goff, Donald C; Holden, Arthur L; Kelly, Deanna L; Malhotra, Anil K; Nielsen, Jimmi; Pirmohamed, Munir; Rujescu, Dan; Werge, Thomas; Levy, Deborah L; Josiassen, Richard C; Kennedy, James L; Lieberman, Jeffrey A; Daly, Mark J; Sullivan, Patrick F; Chambert, K D; Collier, D A; Genovese, G; Giegling, I; Holmans, P; Jonasdottir, A; Kirov, G; McCarroll, S A; MacCabe, J H; Mantripragada, K; Moran, J L; Neale, B M; Stefansson, H; Rujescu, D; Daly, M J; Sullivan, P F; Owen, M J; O'Donovan, M C; Walters, J T R

2017-01-01

The antipsychotic clozapine is uniquely effective in the management of schizophrenia; however, its use is limited by its potential to induce agranulocytosis. The causes of this, and of its precursor neutropenia, are largely unknown, although genetic factors have an important role. We sought risk alleles for clozapine-associated neutropenia in a sample of 66 cases and 5583 clozapine-treated controls, through a genome-wide association study (GWAS), imputed human leukocyte antigen (HLA) alleles, exome array and copy-number variation (CNV) analyses. We then combined associated variants in a meta-analysis with data from the Clozapine-Induced Agranulocytosis Consortium (up to 163 cases and 7970 controls). In the largest combined sample to date, we identified a novel association with rs149104283 (odds ratio (OR)=4.32, P=1.79 × 10−8), intronic to transcripts of SLCO1B3 and SLCO1B7, members of a family of hepatic transporter genes previously implicated in adverse drug reactions including simvastatin-induced myopathy and docetaxel-induced neutropenia. Exome array analysis identified gene-wide associations of uncommon non-synonymous variants within UBAP2 and STARD9. We additionally provide independent replication of a previously identified variant in HLA-DQB1 (OR=15.6, P=0.015, positive predictive value=35.1%). These results implicate biological pathways through which clozapine may act to cause this serious adverse effect. PMID:27400856

Low-Cost Phased Array Antenna for Sounding Rockets, Missiles, and Expendable Launch Vehicles

NASA Technical Reports Server (NTRS)

Mullinix, Daniel; Hall, Kenneth; Smith, Bruce; Corbin, Brian

2012-01-01

A low-cost beamformer phased array antenna has been developed for expendable launch vehicles, rockets, and missiles. It utilizes a conformal array antenna of ring or individual radiators (design varies depending on application) that is designed to be fed by the recently developed hybrid electrical/mechanical (vendor-supplied) phased array beamformer. The combination of these new array antennas and the hybrid beamformer results in a conformal phased array antenna that has significantly higher gain than traditional omni antennas, and costs an order of magnitude or more less than traditional phased array designs. Existing omnidirectional antennas for sounding rockets, missiles, and expendable launch vehicles (ELVs) do not have sufficient gain to support the required communication data rates via the space network. Missiles and smaller ELVs are often stabilized in flight by a fast (i.e. 4 Hz) roll rate. This fast roll rate, combined with vehicle attitude changes, greatly increases the complexity of the high-gain antenna beam-tracking problem. Phased arrays for larger ELVs with roll control are prohibitively expensive. Prior techniques involved a traditional fully electronic phased array solution, combined with highly complex and very fast inertial measurement unit phased array beamformers. The functional operation of this phased array is substantially different from traditional phased arrays in that it uses a hybrid electrical/mechanical beamformer that creates the relative time delays for steering the antenna beam via a small physical movement of variable delay lines. This movement is controlled via an innovative antenna control unit that accesses an internal measurement unit for vehicle attitude information, computes a beam-pointing angle to the target, then points the beam via a stepper motor controller. The stepper motor on the beamformer controls the beamformer variable delay lines that apply the appropriate time delays to the individual array elements to properly steer the beam. The array of phased ring radiators is unique in that it provides improved gain for a small rocket or missile that uses spin stabilization for stability. The antenna pattern created is symmetric about the roll axis (like an omnidirectional wraparound), and is thus capable of providing continuous coverage that is compatible with very fast spinning rockets. For larger ELVs with roll control, a linear array of elements can be used for the 1D scanned beamformer and phased array, or a 2D scanned beamformer can be used with an NxN element array.

Laser diode arrays for naval reconnaissance

NASA Astrophysics Data System (ADS)

Holloway, John H., Jr.; Crosby, Frank J.; Petee, Danny A.; Suiter, Harold R.; Witherspoon, Ned H.

2003-09-01

The Airborne Littoral Reconnaissance Technologies (ALRT) Project has demonstrated a nighttime operational minefield detection capability using commercial off-the-shelf high-power Laser Diode Arrays (LDAs). Historically, optical aerial detection of minefields has primarily been limited to daytime operations but LDAs promise compact and efficient lighting to allow for enhanced reconnaissance operations for future mine detection systems. When combined with high-resolution intensified imaging systems, LDAs can illuminate otherwise unseen areas. Future wavelength options will open the way for active multispectral imaging with LDAs. The Coastal Systems Station working for the Office of Naval Research on the ALRT project has designed, developed, integrated, and tested both prototype and commercial arrays from a Cessna airborne platform. Detailed test results show the ability to detect several targets of interest in a variety of background conditions. Initial testing of the prototype arrays, reported on last year, was completed and further investigations of the commercial versions were performed. Polarization-state detection studies were performed, and advantageous properties of the source-target-sensor geometry noted. Current project plans are to expand the field-of-view coverage for Naval exercises in the summer of 2003. This paper describes the test collection, data library products, array information, on-going test analysis results, and future planned testing of the LDAs.

Status of air-shower measurements with sparse radio arrays

NASA Astrophysics Data System (ADS)

Schröder, Frank G.

2017-03-01

This proceeding gives a summary of the current status and open questions of the radio technique for cosmic-ray air showers, assuming that the reader is already familiar with the principles. It includes recent results of selected experiments not present at this conference, e.g., LOPES and TREND. Current radio arrays like AERA or Tunka-Rex have demonstrated that areas of several km2 can be instrumented for reasonable costs with antenna spacings of the order of 200m. For the energy of the primary particle such sparse antenna arrays can already compete in absolute accuracy with other precise techniques, like the detection of air-fluorescence or air-Cherenkov light. With further improvements in the antenna calibration, the radio detection might become even more accurate. For the atmospheric depth of the shower maximum, Xmax, currently only the dense array LOFAR features a precision similar to the fluorescence technique, but analysis methods for the radio measurement of Xmax are still under development. Moreover, the combination of radio and muon measurements is expected to increase the accuracy of the mass composition, and this around-the-clock recording is not limited to clear nights as are the light-detection methods. Consequently, radio antennas will be a valuable add-on for any air shower array targeting the energy range above 100 PeV.

Wide-bandwidth high-resolution search for extraterrestrial intelligence

NASA Technical Reports Server (NTRS)

Horowitz, Paul

1995-01-01

Research was accomplished during the third year of the grant on: BETA architecture, an FFT array, a feature extractor, the Pentium array and workstation, and a radio astronomy spectrometer. The BETA (this SETI project) system architecture has been evolving generally in the direction of greater robustness against terrestrial interference. The new design adds a powerful state-memory feature, multiple simultaneous thresholds, and the ability to integrate multiple spectra in a flexible state-machine architecture. The FFT array is reported with regards to its hardware verification, array production, and control. The feature extractor is responsible for maintaining a moving baseline, recognizing large spectral peaks, following the progress of previously identified interesting spectral regions, and blocking signals from regions previously identified as containing interference. The Pentium array consists of 21 Pentium-based PC motherboards, each with 16 MByte of RAM and an Ethernet interface. Each motherboard receives and processes the data from a feature extractor/correlator board set, passing on the results of a first analysis to the central Unix workstation (through which each is also booted). The radio astronomy spectrometer is a technological spinoff from SETI work. It is proposed to be a combined spectrometer and power-accumulator, for use at Arecibo Observatory to search for neutral hydrogen emission from condensations of neutral hydrogen at high redshift (z = 5).

Electronic warfare antenna systems - Past and present

NASA Astrophysics Data System (ADS)

Yaw, D.

1981-09-01

In discussing fixed beam arrays, it is noted that an array may be used to create simultaneous fixed beams or to form asymmetric beams of a desired shape. Attention is also given to arrays and beam control, noting that for some electronic warfare applications combinations of broad and narrow beam antenna response are needed. Other topics include ECM jamming antenna techniques and advanced array systems.

Coherent beam combining of collimated fiber array based on target-in-the-loop technique

NASA Astrophysics Data System (ADS)

Li, Xinyang; Geng, Chao; Zhang, Xiaojun; Rao, Changhui

2011-11-01

Coherent beam combining (CBC) of fiber array is a promising way to generate high power and high quality laser beams. Target-in-the-loop (TIL) technique might be an effective way to ensure atmosphere propagation compensation without wavefront sensors. In this paper, we present very recent research work about CBC of collimated fiber array using TIL technique at the Key Lab on Adaptive Optics (KLAO), CAS. A novel Adaptive Fiber Optics Collimator (AFOC) composed of phase-locking module and tip/tilt control module was developed. CBC experimental setup of three-element fiber array was established. Feedback control is realized using stochastic parallel gradient descent (SPGD) algorithm. The CBC based on TIL with piston and tip/tilt correction simultaneously is demonstrated. And the beam pointing to locate or sweep position of combined spot on target was achieved through TIL technique too. The goal of our work is achieve multi-element CBC for long-distance transmission in atmosphere.

Large Ka-Band Slot Array for Digital Beam-Forming Applications

NASA Technical Reports Server (NTRS)

Rengarajan, Sembiam; Zawadzki, Mark S.; Hodges, Richard E.

2011-01-01

This work describes the development of a large Ka Band Slot Array for the Glacier and Land Ice Surface Topography Interferometer (GLISTIN), a proposed spaceborne interferometric synthetic aperture radar for topographic mapping of ice sheets and glaciers. GLISTIN will collect ice topography measurement data over a wide swath with sub-seasonal repeat intervals using a Ka-band digitally beamformed antenna. For technology demonstration purpose a receive array of size 1x1 m, consisting of 160x160 radiating elements, was developed. The array is divided into 16 sticks, each stick consisting of 160x10 radiating elements, whose outputs are combined to produce 16 digital beams. A transmit array stick was also developed. The antenna arrays were designed using Elliott's design equations with the use of an infinite-array mutual-coupling model. A Floquet wave model was used to account for external coupling between radiating slots. Because of the use of uniform amplitude and phase distribution, the infinite array model yielded identical values for all radiating elements but for alternating offsets, and identical coupling elements but for alternating positive and negative tilts. Waveguide-fed slot arrays are finding many applications in radar, remote sensing, and communications applications because of their desirable properties such as low mass, low volume, and ease of design, manufacture, and deployability. Although waveguide-fed slot arrays have been designed, built, and tested in the past, this work represents several advances to the state of the art. The use of the infinite array model for the radiating slots yielded a simple design process for radiating and coupling slots. Method of moments solution to the integral equations for alternating offset radiating slots in an infinite array environment was developed and validated using the commercial finite element code HFSS. For the analysis purpose, a method of moments code was developed for an infinite array of subarrays. Overall the 1x1 m array was found to be successful in meeting the objectives of the GLISTIN demonstration antenna, especially with respect to the 0.042deg, 1/10th of the beamwidth of each stick, relative beam alignment between sticks.

Method for monitoring environmental and corrosion

DOEpatents

Glass, R.S.; Clarke, W.L. Jr.; Ciarlo, D.R.

1995-08-01

A corrosion sensor array is described incorporating individual elements for measuring various elements and ions, such as chloride, sulfide, copper, hydrogen (pH), etc. and elements for evaluating the instantaneous corrosion properties of structural materials. The exact combination and number of elements measured or monitored would depend upon the environmental conditions and materials used which are subject to corrosive effects. Such a corrosion monitoring system embedded in or mounted on a structure exposed to the environment would serve as an early warning system for the onset of severe corrosion problems for the structure, thus providing a safety factor as well as economic factors. The sensor array is accessed to an electronics/computational system, which provides a means for data collection and analysis. 7 figs.

Analysis of S-band solid-state transmitters for the solar power satellite

NASA Technical Reports Server (NTRS)

Belohoubek, E. F.; Ettenberg, M.; Huang, H. C.; Nowogrodzki, M.; Sechi, F. N.

1979-01-01

The possibility of replacing the Reference System antenna in which thermionic devices are used for the dc-to-microwave conversion, with solid-state elements was explored. System, device, and antenna module tradeoff investigations strongly point toward the desirability of changing the transmitter concept to a distributed array of relatively low power elements, deriving their dc power directly from the solar cell array and whose microwave power outputs are combined in space. The approach eliminates the thermal, weight, and dc-voltage distribution problems of a system in which high power tubes are simply replaced with clusters of solid state amplifiers. The proposed approach retains the important advantages of a solid state system: greatly enhanced reliability and graceful degradation of the system.

Tunable plasmon-induced transparency with graphene-based T-shaped array metasurfaces

NASA Astrophysics Data System (ADS)

Niu, Yuying; Wang, Jicheng; Hu, Zhengda; Zhang, Feng

2018-06-01

The frequency tunable Plasmonic induced transparency (PIT) effect is researched with a periodically patterned T-shaped graphene array in mid-infrared region. We adjust the geometrical parameters to obtain the optimized combination for the realization of the PIT response and use the coupled Lorentz oscillator model to analysis the physical mechanism. Due to the properties of graphene, the PIT effect can be easily and markedly enhanced with the increase of chemical potential and carrier mobility. The frequency of PIT effect is also insensitive with the angle of incident light. In addition, we also propose the π shaped structure to realizing the double-peak PIT effect. The results offer a flexible approach for the development of tunable graphene-based photonic devices.

Artifact Suppression in Imaging of Myocardial Infarction Using B1-Weighted Phased-Array Combined Phase-Sensitive Inversion Recovery

PubMed Central

Kellman, Peter; Dyke, Christopher K.; Aletras, Anthony H.; McVeigh, Elliot R.; Arai, Andrew E.

2007-01-01

Regions of the body with long T1, such as cerebrospinal fluid (CSF), may create ghost artifacts on gadolinium-hyperenhanced images of myocardial infarction when inversion recovery (IR) sequences are used with a segmented acquisition. Oscillations in the transient approach to steady state for regions with long T1 may cause ghosts, with the number of ghosts being equal to the number of segments. B1-weighted phased-array combining provides an inherent degree of ghost artifact suppression because the ghost artifact is weighted less than the desired signal intensity by the coil sensitivity profiles. Example images are shown that illustrate the suppression of CSF ghost artifacts by the use of B1-weighted phased-array combining of multiple receiver coils. PMID:14755669

All optical programmable logic array (PLA)

NASA Astrophysics Data System (ADS)

Hiluf, Dawit

2018-03-01

A programmable logic array (PLA) is an integrated circuit (IC) logic device that can be reconfigured to implement various kinds of combinational logic circuits. The device has a number of AND and OR gates which are linked together to give output or further combined with more gates or logic circuits. This work presents the realization of PLAs via the physics of a three level system interacting with light. A programmable logic array is designed such that a number of different logical functions can be combined as a sum-of-product or product-of-sum form. We present an all optical PLAs with the aid of laser light and observables of quantum systems, where encoded information can be considered as memory chip. The dynamics of the physical system is investigated using Lie algebra approach.

Optical Communications With A Geiger Mode APD Array

DTIC Science & Technology

2016-02-09

spurious fires from numerous sources, including crosstalk from other detectors in the same array . Additionally, after a 9 successful detection, the...be combined into arrays with large numbers of detectors , allowing for scaling of dynamic range with relatively little overhead on space and power...overall higher rate of dark counts than a single detector , this is more than compensated for by the extra detectors . A sufficiently large APD array could

Large Format, Background Limited Arrays of Kinetic Inductance Detectors for Sub-mm Astronomy

NASA Astrophysics Data System (ADS)

Baselmans, Jochem

2018-01-01

We present the development of large format imaging arrays for sub-mm astronomy based upon microwave Kinetic Inductance detectors and their read-out. In particular we focus on the arrays developed for the A-MKID instrument for the APEX telescope. AMKID contains 2 focal plane arrays, covering a field of view of 15?x15?. One array is optimized for the 350 GHz telluric window, the other for the 850 GHz window. Both arrays are constructed from four 61 x 61 mm detector chips, each of which contains up to 3400 detectors and up to 880 detectors per readout line. The detectors are lens antenna coupled MKIDs made from NbTiN and Aluminium that reach photon noise limited sensitivity in combination with a high optical coupling. The lens-antenna radiation coupling enables the use of 4K optics and Lyot stop due to the intrinsic directivity of the detector beam, allowing a simple cryogenic architecture. We discuss the pixel design and verification, detector packaging and the array performance. We will also discuss the readout system, which is a combination of a digital and analog back-end that can read-out up to 4000 pixels simultaneously using frequency division multiplexing.

Semiotic indexing of digital resources

DOEpatents

Parker, Charles T; Garrity, George M

2014-12-02

A method of classifying a plurality of documents. The method includes steps of providing a first set of classification terms and a second set of classification terms, the second set of classification terms being different from the first set of classification terms; generating a first frequency array of a number of occurrences of each term from the first set of classification terms in each document; generating a second frequency array of a number of occurrences of each term from the second set of classification terms in each document; generating a first similarity matrix from the first frequency array; generating a second similarity matrix from the second frequency array; determining an entrywise combination of the first similarity matrix and the second similarity matrix; and clustering the plurality of documents based on the result of the entrywise combination.

Screening combinatorial arrays of inorganic materials with spectroscopy or microscopy

DOEpatents

Schultz, Peter G.; Xiang, Xiaodong; Goldwasser, Isy

2004-02-03

Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

Silicon-on-insulator based nanopore cavity arrays for lipid membrane investigation.

PubMed

Buchholz, K; Tinazli, A; Kleefen, A; Dorfner, D; Pedone, D; Rant, U; Tampé, R; Abstreiter, G; Tornow, M

2008-11-05

We present the fabrication and characterization of nanopore microcavities for the investigation of transport processes in suspended lipid membranes. The cavities are situated below the surface of silicon-on-insulator (SOI) substrates. Single cavities and large area arrays were prepared using high resolution electron-beam lithography in combination with reactive ion etching (RIE) and wet chemical sacrificial underetching. The locally separated compartments have a circular shape and allow the enclosure of picoliter volume aqueous solutions. They are sealed at their top by a 250 nm thin Si membrane featuring pores with diameters from 2 µm down to 220 nm. The Si surface exhibits excellent smoothness and homogeneity as verified by AFM analysis. As biophysical test system we deposited lipid membranes by vesicle fusion, and demonstrated their fluid-like properties by fluorescence recovery after photobleaching. As clearly indicated by AFM measurements in aqueous buffer solution, intact lipid membranes successfully spanned the pores. The nanopore cavity arrays have potential applications in diagnostics and pharmaceutical research on transmembrane proteins.

Polymer arrays from the combinatorial synthesis of novel materials

DOEpatents

Schultz, Peter G.; Xiang, Xiao-Dong; Goldwasser, Isy; Briceno, Gabriel; Sun, Xiao-Dong

2004-09-21

Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

Synthesis and screening combinatorial arrays of zeolites

DOEpatents

Schultz, Peter G.; Xiang, Xiaodong; Goldwasser, Isy

2003-11-18

Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

BODIPY-based fluorometric sensor array for the highly sensitive identification of heavy-metal ions.

PubMed

Niu, Li-Ya; Li, Hui; Feng, Liang; Guan, Ying-Shi; Chen, Yu-Zhe; Duan, Chun-Feng; Wu, Li-Zhu; Guan, Ya-Feng; Tung, Chen-Ho; Yang, Qing-Zheng

2013-05-02

A BODIPY(4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)-based fluorometric sensor array has been developed for the highly sensitive detection of eight heavy-metal ions at micromolar concentration. The di-2-picolyamine (DPA) derivatives combine high affinities for a variety of heavy-metal ions with the capacity to perturb the fluorescence properties of BODIPY, making them perfectly suitable for the design of fluorometric sensor arrays for heavy-metal ions. 12 cross-reactive BODIPY fluorescent indicators provide facile identification of the heavy-metal ions using a standard chemometric approach (hierarchical clustering analysis); no misclassifications were found over 45 trials. Clear differentiation among heavy-metal ions as a function of concentration was also achieved, even down to 10(-7)M. A semi-quantitative interpolation of the heavy-metal concentration is obtained by comparing the total Euclidean distance of the measurement with a set of known concentrations in the library. Copyright © 2013 Elsevier B.V. All rights reserved.

Role of miR-1 expression in clear cell renal cell carcinoma (ccRCC): A bioinformatics study based on GEO, ArrayExpress microarrays and TCGA database.

PubMed

Yan, Hai-Biao; Huang, Jia-Cheng; Chen, You-Rong; Yao, Jian-Ni; Cen, Wei-Ning; Li, Jia-Yi; Jiang, Yi-Fan; Chen, Gang; Li, Sheng-Hua

2018-02-01

To investigate the clinical value and potential molecular mechanisms of miR-1 in clear cell renal cell carcinoma (ccRCC). We searched the Gene Expression Omnibus (GEO), ArrayExpress, several online publication databases and the Cancer Genome Atlas (TCGA). Continuous variable meta-analysis and diagnostic meta-analysis were conducted, both in Stata 14, to show the expression of miR-1 in ccRCC. Furthermore, we acquired the potential targets of miR-1 from datasets that transfected miR-1 into ccRCC cells, online prediction databases, differentially expressed genes from TCGA and literature. Subsequently bioinformatics analysis based on aforementioned selected target genes was conducted. The combined effect was -0.92 with the 95% confidence interval (CI) of -1.08 to -0.77 based on fixed effect model (I 2  = 81.3%, P < 0.001). No publication bias was found in our investigation. Sensitivity analysis showed that GSE47582 and 2 TCGA studies might cause heterogeneity. After eliminating them, the combined effect was -0.47 (95%CI: -0.78, -0.16) with I 2  = 18.3%. As for the diagnostic meta-analysis, the combined sensitivity and specificity were 0.90 (95%CI: 0.61, 0.98) and 0.63 (95%CI: 0.39, 0.82). The area under the curve (AUC) in the summarized receiver operating characteristic (SROC) curve was 0.83 (95%CI: 0.80, 0.86). No publication bias was found (P = 0.15). We finally got 67 genes which were defined the promising target genes of miR-1 in ccRCC. The most three significant KEGG pathways based on the aforementioned genes were Complement and coagulation cascades, ECM-receptor interaction and Focal adhesion. The downregulation of miR-1 might play an important role in ccRCC by targeting its target genes. Copyright © 2017 Elsevier GmbH. All rights reserved.

Classification of the medicinal plants of the genus Atractylodes using high-performance liquid chromatography with diode array and tandem mass spectrometry detection combined with multivariate statistical analysis.

PubMed

Cho, Hyun-Deok; Kim, Unyong; Suh, Joon Hyuk; Eom, Han Young; Kim, Junghyun; Lee, Seul Gi; Choi, Yong Seok; Han, Sang Beom

2016-04-01

Analytical methods using high-performance liquid chromatography with diode array and tandem mass spectrometry detection were developed for the discrimination of the rhizomes of four Atractylodes medicinal plants: A. japonica, A. macrocephala, A. chinensis, and A. lancea. A quantitative study was performed, selecting five bioactive components, including atractylenolide I, II, III, eudesma-4(14),7(11)-dien-8-one and atractylodin, on twenty-six Atractylodes samples of various origins. Sample extraction was optimized to sonication with 80% methanol for 40 min at room temperature. High-performance liquid chromatography with diode array detection was established using a C18 column with a water/acetonitrile gradient system at a flow rate of 1.0 mL/min, and the detection wavelength was set at 236 nm. Liquid chromatography with tandem mass spectrometry was applied to certify the reliability of the quantitative results. The developed methods were validated by ensuring specificity, linearity, limit of quantification, accuracy, precision, recovery, robustness, and stability. Results showed that cangzhu contained higher amounts of atractylenolide I and atractylodin than baizhu, and especially atractylodin contents showed the greatest variation between baizhu and cangzhu. Multivariate statistical analysis, such as principal component analysis and hierarchical cluster analysis, were also employed for further classification of the Atractylodes plants. The established method was suitable for quality control of the Atractylodes plants. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays

PubMed Central

Menten, Björn; Pattyn, Filip; De Preter, Katleen; Robbrecht, Piet; Michels, Evi; Buysse, Karen; Mortier, Geert; De Paepe, Anne; van Vooren, Steven; Vermeesch, Joris; Moreau, Yves; De Moor, Bart; Vermeulen, Stefan; Speleman, Frank; Vandesompele, Jo

2005-01-01

Background The availability of the human genome sequence as well as the large number of physically accessible oligonucleotides, cDNA, and BAC clones across the entire genome has triggered and accelerated the use of several platforms for analysis of DNA copy number changes, amongst others microarray comparative genomic hybridization (arrayCGH). One of the challenges inherent to this new technology is the management and analysis of large numbers of data points generated in each individual experiment. Results We have developed arrayCGHbase, a comprehensive analysis platform for arrayCGH experiments consisting of a MIAME (Minimal Information About a Microarray Experiment) supportive database using MySQL underlying a data mining web tool, to store, analyze, interpret, compare, and visualize arrayCGH results in a uniform and user-friendly format. Following its flexible design, arrayCGHbase is compatible with all existing and forthcoming arrayCGH platforms. Data can be exported in a multitude of formats, including BED files to map copy number information on the genome using the Ensembl or UCSC genome browser. Conclusion ArrayCGHbase is a web based and platform independent arrayCGH data analysis tool, that allows users to access the analysis suite through the internet or a local intranet after installation on a private server. ArrayCGHbase is available at . PMID:15910681

EML Array fabricated by SAG technique monolithically integrated with a buried ridge AWG multiplexer

NASA Astrophysics Data System (ADS)

Xu, Junjie; Liang, Song; Zhang, Zhike; An, Junming; Zhu, Hongliang; Wang, Wei

2017-06-01

We report the fabrication of a ten channel electroabsorption modulated DFB laser (EML) array. Different emission wavelengths of the laser array are obtained by selective area growth (SAG) technique, which is also used for the integration of electroabsorption modulators (EAM) with the lasers. An arrayed waveguide grating (AWG) combiner is integrated monolithically with the laser array by butt-joint regrowth (BJR) technique. A buried ridge waveguide structure is adopted for the AWG combiner. A self aligned fabrication procedure is adopted for the fabrication of the waveguide structure of the device to eliminate the misalignment between the laser active waveguide and the passive waveguide. A Ti thin film heater is integrated for each laser in the array. With the help of the heaters, ten laser emissions with 1.8 nm channel spacing are obtained. The integrated EAM has a larger than 11 dB static extinction ratios and larger than 8 GHz small signal modulation bandwidths. The light power collected in the output waveguide of the AWG is larger than -13 dBm for each wavelength.

A 16-channel combined loop-dipole transceiver array for 7 Tesla body MRI.

PubMed

Ertürk, M Arcan; Raaijmakers, Alexander J E; Adriany, Gregor; Uğurbil, Kâmil; Metzger, Gregory J

2017-02-01

To develop a 16-channel transceive body imaging array at 7.0 T with improved transmit, receive, and specific absorption rate (SAR) performance by combining both loop and dipole elements and using their respective and complementary near and far field characteristics. A 16-channel radiofrequency (RF) coil array consisting of eight loop-dipole blocks (16LD) was designed and constructed. Transmit and receive performance was quantitatively investigated in phantom and human model simulations, and experiments on five healthy volunteers inside the prostate. Comparisons were made with 16-channel microstrip line (16ML) and 10-channel fractionated dipole antenna (10DA) arrays. The 16LD was used to acquire anatomic and functional images of the prostate, kidneys, and heart. The 16LD provided > 14% improvements in the signal-to-noise ratio (SNR), peak B1+, B1+ transmit, and SAR efficiencies over the 16ML and 10DA in simulations inside the prostate. Experimentally, the 16LD had > 20% higher SNR and B1+ transmit efficiency compared with other arrays, and achieved up to 51.8% higher peak B1+ compared with 10DA. Combining loop and dipole elements provided a body imaging array with high channel count and density while limiting inter-element coupling. The 16LD improved both near and far-field performance compared with existing 7.0T body arrays and provided high-quality MRI of the prostate kidneys and heart. Magn Reson Med 77:884-894, 2017. © 2016 International Society for Magnetic Resonance in Medicine. © 2016 International Society for Magnetic Resonance in Medicine.

Photogrammetric Assessment of the Hubble Space Telescope Solar Arrays During the Second Servicing Mission

NASA Technical Reports Server (NTRS)

Sapp, C. A.; Dragg, J. L.; Snyder, M. W.; Gaunce, M. T.; Decker, J. E.

1998-01-01

This report documents the photogrammetric assessment of the Hubble Space Telescope (HST) solar arrays conducted by the NASA c Center Image Science and Analysis Group during Second Servicing Mission 2 (SM-2) on STS-82 in February 1997. Two type solar array analyses were conducted during the mission using Space Shuttle payload bay video: (1) measurement of solar array motion due to induced loads, and (2) measurement of the solar array static or geometric twist caused by the cumulative array loading. The report describes pre-mission planning and analysis technique development activities conducted to acquire and analyze solar array imagery data during SM-2. This includes analysis of array motion obtained during SM-1 as a proof-of-concept of the SM-2 measurement techniques. The report documents the results of real-time analysis conducted during the mission and subsequent analysis conducted post-flight. This report also provides a summary of lessons learned on solar array imagery analysis from SM-2 and recommendations for future on-orbit measurements applicable to HST SM-3 and to the International Space Station. This work was performed under the direction of the Goddard Space Flight Center HST Flight Systems and Servicing Project.

Analysis of Roll Steering for Solar Electric Propulsion Missions

NASA Technical Reports Server (NTRS)

Pederson, Dylan, M.; Hojnicki, Jeffrey, S.

2012-01-01

Nothing is more vital to a spacecraft than power. Solar Electric Propulsion (SEP) uses that power to provide a safe, reliable, and, most importantly, fuel efficient means to propel a spacecraft to its destination. The power performance of an SEP vehicle s solar arrays and electrical power system (EPS) is largely influenced by the environment in which the spacecraft is operating. One of the most important factors that determines solar array power performance is how directly the arrays are pointed to the sun. To get the most power from the solar arrays, the obvious solution is to point them directly at the sun at all times. Doing so is not a problem in deep space, as the environment and pointing conditions that a spacecraft faces are fairly constant and are easy to accommodate, if necessary. However, large and sometimes rapid variations in environmental and pointing conditions are experienced by Earth orbiting spacecraft. SEP spacecraft also have the additional constraint of needing to keep the thrust vector aligned with the velocity vector. Thus, it is important to analyze solar array power performance for any vehicle that spends an extended amount of time orbiting the Earth, and to determine how much off-pointing can be tolerated to produce the required power for a given spacecraft. This paper documents the benefits and drawbacks of perfectly pointing the solar arrays of an SEP spacecraft spiraling from Earth orbit, and how this might be accomplished. Benefits and drawbacks are defined in terms of vehicle mass, power, volume, complexity, and cost. This paper will also look at the application of various solar array pointing methods to future missions. One such pointing method of interest is called roll steering . Roll steering involves rolling the entire vehicle twice each orbit. Roll steering, combined with solar array gimbal tracking, is used to point the solar arrays perfectly towards the sun at all points in the orbit, while keeping the vehicle thrusters aligned in the velocity direction. Roll steering is particularly attractive for a recently proposed mission that involves a spiral trajectory from low Earth orbit (LEO) to the Earth-Moon Lagrange Point 1 (E-M L1). During the spiral, the spacecraft will spend over 300 days experiencing the full spectrum of near-earth environments and solar array pointing conditions. An extensive study of the application of SEP (and roll steering) to this spiral mission is included, highlighting the ultimate goal of reduced vehicle cost and mass. Tools used for this analysis include the Systems Power Analysis for Capability Evaluation (Refs. 1 and 2) (SPACE) electrical power systems code, and SEP trajectory simulation tools developed at NASA Glenn Research Center.

Elastic and Inelastic Scattering of Neutrons using a CLYC array

NASA Astrophysics Data System (ADS)

Brown, Tristan; Doucet, E.; Chowdhury, P.; Lister, C. J.; Wilson, G. L.; Devlin, M.; Mosby, S.

2015-10-01

CLYC scintillators, which have dual neutron and gamma response, have recently ushered in the possibility of fast neutron spectroscopy without time-of-flight (TOF). A 16-element array of 1'' x 1'' 6Li-depleted CLYC crystals, where pulse-shape-discrimination is achieved via digital pulse processing, has been commissioned at UMass Lowell. In an experiment at LANSCE, high energy neutrons were used to bombard 56Fe and 238U targets, in order to measure elastic and inelastic neutron scattering cross sections as a function of energy and angle with the array. The array is placed very close to the targets for enhanced geometrical solid angles for scattered neutrons compared to standard neutron-TOF measurements. A pulse-height spectrum of scattered neutrons in the detectors is compared to the energy of the incident neutrons, which is measured via the TOF of the pulsed neutrons from the source to the detectors. Recoil corrections are necessary to combine the energy spectra from all the detectors to obtain angle-integrated elastic and inelastic cross-sections. The detection techniques, analysis procedures and results will be presented. Supported by NNSA-SSAA program through DOE Grant DE-NA00013008.

Copy number variants analysis in a cohort of isolated and syndromic developmental delay/intellectual disability reveals novel genomic disorders, position effects and candidate disease genes.

PubMed

Di Gregorio, E; Riberi, E; Belligni, E F; Biamino, E; Spielmann, M; Ala, U; Calcia, A; Bagnasco, I; Carli, D; Gai, G; Giordano, M; Guala, A; Keller, R; Mandrile, G; Arduino, C; Maffè, A; Naretto, V G; Sirchia, F; Sorasio, L; Ungari, S; Zonta, A; Zacchetti, G; Talarico, F; Pappi, P; Cavalieri, S; Giorgio, E; Mancini, C; Ferrero, M; Brussino, A; Savin, E; Gandione, M; Pelle, A; Giachino, D F; De Marchi, M; Restagno, G; Provero, P; Cirillo Silengo, M; Grosso, E; Buxbaum, J D; Pasini, B; De Rubeis, S; Brusco, A; Ferrero, G B

2017-10-01

Array-comparative genomic hybridization (array-CGH) is a widely used technique to detect copy number variants (CNVs) associated with developmental delay/intellectual disability (DD/ID). Identification of genomic disorders in DD/ID. We performed a comprehensive array-CGH investigation of 1,015 consecutive cases with DD/ID and combined literature mining, genetic evidence, evolutionary constraint scores, and functional information in order to assess the pathogenicity of the CNVs. We identified non-benign CNVs in 29% of patients. Amongst the pathogenic variants (11%), detected with a yield consistent with the literature, we found rare genomic disorders and CNVs spanning known disease genes. We further identified and discussed 51 cases with likely pathogenic CNVs spanning novel candidate genes, including genes encoding synaptic components and/or proteins involved in corticogenesis. Additionally, we identified two deletions spanning potential Topological Associated Domain (TAD) boundaries probably affecting the regulatory landscape. We show how phenotypic and genetic analyses of array-CGH data allow unraveling complex cases, identifying rare disease genes, and revealing unexpected position effects. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Solution-based analysis of multiple analytes by a sensor array: toward the development of an electronic tongue

NASA Astrophysics Data System (ADS)

Savoy, Steven M.; Lavigne, John J.; Yoo, J. S.; Wright, John; Rodriguez, Marc; Goodey, Adrian; McDoniel, Bridget; McDevitt, John T.; Anslyn, Eric V.; Shear, Jason B.; Ellington, Andrew D.; Neikirk, Dean P.

1998-12-01

A micromachined sensor array has been developed for the rapid characterization of multi-component mixtures in aqueous media. The sensor functions in a manner analogous to that of the mammalian tongue, using an array composed of individually immobilized polystyrene-polyethylene glycol composite microspheres selectively arranged in micromachined etch cavities localized o n silicon wafers. Sensing occurs via colorimetric or fluorometric changes to indicator molecules that are covalently bound to amine termination sites on the polymeric microspheres. The hybrid micromachined structure has been interfaced directly to a charged-coupled-device that is used for the simultaneous acquisition of the optical data from the individually addressable `taste bud' elements. With the miniature sensor array, acquisition of data streams composed of red, green, and blue color patterns distinctive for the analytes in the solution are rapidly acquired. The unique combination of carefully chosen reporter molecules with water permeable microspheres allows for the simultaneous detection and quantification of a variety of analytes. The fabrication of the sensor structures and the initial colorimetric and fluorescent responses for pH, Ca+2, Ce+3, and sugar are reported. Interface to microfluidic components should also be possible, producing a complete sampling/sensing system.

Parallel, confocal, and complete spectrum imager for fluorescent detection of high-density microarray

NASA Astrophysics Data System (ADS)

Bogdanov, Valery L.; Boyce-Jacino, Michael

1999-05-01

Confined arrays of biochemical probes deposited on a solid support surface (analytical microarray or 'chip') provide an opportunity to analysis multiple reactions simultaneously. Microarrays are increasingly used in genetics, medicine and environment scanning as research and analytical instruments. A power of microarray technology comes from its parallelism which grows with array miniaturization, minimization of reagent volume per reaction site and reaction multiplexing. An optical detector of microarray signals should combine high sensitivity, spatial and spectral resolution. Additionally, low-cost and a high processing rate are needed to transfer microarray technology into biomedical practice. We designed an imager that provides confocal and complete spectrum detection of entire fluorescently-labeled microarray in parallel. Imager uses microlens array, non-slit spectral decomposer, and high- sensitive detector (cooled CCD). Two imaging channels provide a simultaneous detection of localization, integrated and spectral intensities for each reaction site in microarray. A dimensional matching between microarray and imager's optics eliminates all in moving parts in instrumentation, enabling highly informative, fast and low-cost microarray detection. We report theory of confocal hyperspectral imaging with microlenses array and experimental data for implementation of developed imager to detect fluorescently labeled microarray with a density approximately 103 sites per cm2.

Application of a single area array detector for acquistion, tracking and point-ahead in space optical communications

NASA Technical Reports Server (NTRS)

Clark, D. L.; Cosgrove, M.; Vanvranken, R.; Park, H.; Fitzmaurice, M.

1989-01-01

Functions of acquisition, tracking, and point-ahead in space optical communications are being combined into a single system utilizing an area array detector. An analysis is presented of the feasibility concept. The key parameters are: optical power less than 1 pW at 0.86 micrometer, acquisition in less than 30 seconds in an acquisition field of view (FOV) of 1 mrad, tracking with 0.5 microrad rms noise at 1000 Hz update rate, and point ahead transfer function precision of 0.25 microrad over a region of 150 microrad. Currently available array detectors were examined. The most demanding specifications are low output noise, a high detection efficiency, a large number of pixels, and frame rates over 1kHz. A proof of concept (POC) demonstration system is currently being built utilizing the Kodak HS-40 detector (a 128 x 128 photodiode array with a 64 channel CCD readout architecture which can be operated at frame rates as high as 40,000/sec). The POC system implements a windowing scheme and special purpose digital signal processing electronic for matched filter acquisition and tracking algorithms.

Analysis of the effects of simulated synergistic LEO environment on solar panels

NASA Astrophysics Data System (ADS)

Allegri, G.; Corradi, S.; Marchetti, M.; Scaglione, S.

2007-02-01

The effects due to the LEO environment exposure of a solar array primary structure are here presented and discussed in detail. The synergistic damaging components featuring LEO environment are high vacuum, thermal cycling, neutral gas, ultraviolet (UV) radiation and cold plasma. The synergistic effects due to these environmental elements are simulated by "on ground" tests, performed in the Space Environment Simulator (SAS) at the University of Rome "La Sapienza"; numerical simulations are performed by the Space Environment Information System (SPENVIS), developed by the European Space Agency (ESA). A "safe life" design for a solar array primary structure is developed, taking into consideration the combined damaging action of the LEO environment components; therefore results from both numerical and experimental simulations are coupled within the framework of a standard finite element method (FEM) based design. The expected durability of the solar array primary structure, made of laminated sandwich composite, is evaluated assuming that the loads exerted on the structure itself are essentially dependent on thermo-elastic stresses. The optical degradation of surface materials and the stiffness and strength degradation of structural elements are taken into account to assess the global structural durability of the solar array under characteristic operative conditions in LEO environment.

Comparison of Two Detection Combination Algorithms for Phased Array Radars

DTIC Science & Technology

2015-07-01

data were generated by a simulator of multi-function radar ( MFR ) and the combination algorithms are evaluated with the recorded simulation data. With...electronically scanned phased array Multi-Function Radar ( MFR ), is a type of radar whose transmitter and receiver functions are composed of numerous...small transmit/receive modules. An MFR can perform many functions previously performed by individual, dedicated radars for search, tracking and

Vortex motion and dynamical states in Josephson arrays

NASA Astrophysics Data System (ADS)

Trias, Enrique

Underdamped Josephson junction arrays are used as model systems to study novel nonlinear effects. A combination of experiments, numerical simulations, and analytical analysis is used to probe different nonlinear behavior such as intrinsic localized modes, resonances in fully frustrated arrays, Meissner-like states, and vortex ratchets. Circuit models of Josephson networks are also developed, and applied to the design and measurement of parallel array oscillators. Ladder arrays have been used for an experimental study of intrinsic localized modes, or discrete breathers. Measurements of breather stability indicate that the maximum allowable bias current is proportional to the array depinning current while the minimum current is related to a junction retrapping mechanism. This retrapping instability usually leads to the formation of multi-site breathers. Collisions between the two nonlinear excitations in ladder arrays, discrete breathers and vortices, have also been numerically investigated. Discrete breathers act as pinning centers to vortex motion and the collisions can be modeled by an energy barrier activation process. When vortices are thermally induced over this barrier, a two-site breather is created. Experiments also reveal remarkable similarities among the do current-voltage characteristics of several kinds of square and triangular arrays, where two resonant voltages are observed. Simulations indicate that at full frustration a dynamical checkerboard state underlies these similarities. For such a traveling solution, the governing equations of the arrays are reduced to three coupled pendulum equations that have two characteristic resonant frequencies. Finally, a kink ratchet potential has been designed using a parallel array of Josephson junctions with alternating cell inductances and junctions areas. Experiments show that the depinning current depends on the direction of the applied current. Other properties of the depinning current versus applied field, such as a long period and a lack of reflection symmetry, have been observed and explained analytically. (Copies available exclusively from MIT Libraries, Rm. 14-0551, Cambridge, MA 02139- 4307. Ph. 617-253-5668; Fax 617-253-1690.)

Development of a Medium Density Combined-Species SNP Array for Pacific and European Oysters (Crassostrea gigas and Ostrea edulis).

PubMed

Gutierrez, Alejandro P; Turner, Frances; Gharbi, Karim; Talbot, Richard; Lowe, Natalie R; Peñaloza, Carolina; McCullough, Mark; Prodöhl, Paulo A; Bean, Tim P; Houston, Ross D

2017-07-05

SNP arrays are enabling tools for high-resolution studies of the genetic basis of complex traits in farmed and wild animals. Oysters are of critical importance in many regions from both an ecological and economic perspective, and oyster aquaculture forms a key component of global food security. The aim of our study was to design a combined-species, medium density SNP array for Pacific oyster ( Crassostrea gigas ) and European flat oyster ( Ostrea edulis ), and to test the performance of this array on farmed and wild populations from multiple locations, with a focus on European populations. SNP discovery was carried out by whole-genome sequencing (WGS) of pooled genomic DNA samples from eight C. gigas populations, and restriction site-associated DNA sequencing (RAD-Seq) of 11 geographically diverse O. edulis populations. Nearly 12 million candidate SNPs were discovered and filtered based on several criteria, including preference for SNPs segregating in multiple populations and SNPs with monomorphic flanking regions. An Affymetrix Axiom Custom Array was created and tested on a diverse set of samples ( n = 219) showing ∼27 K high quality SNPs for C. gigas and ∼11 K high quality SNPs for O. edulis segregating in these populations. A high proportion of SNPs were segregating in each of the populations, and the array was used to detect population structure and levels of linkage disequilibrium (LD). Further testing of the array on three C. gigas nuclear families ( n = 165) revealed that the array can be used to clearly distinguish between both families based on identity-by-state (IBS) clustering parental assignment software. This medium density, combined-species array will be publicly available through Affymetrix, and will be applied for genome-wide association and evolutionary genetic studies, and for genomic selection in oyster breeding programs. Copyright © 2017 Gutierrez et al.

Structural control of ultra-fine CoPt nanodot arrays via electrodeposition process

NASA Astrophysics Data System (ADS)

Wodarz, Siggi; Hasegawa, Takashi; Ishio, Shunji; Homma, Takayuki

2017-05-01

CoPt nanodot arrays were fabricated by combining electrodeposition and electron beam lithography (EBL) for the use of bit-patterned media (BPM). To achieve precise control of deposition uniformity and coercivity of the CoPt nanodot arrays, their crystal structure and magnetic properties were controlled by controlling the diffusion state of metal ions from the initial deposition stage with the application of bath agitation. Following bath agitation, the composition gradient of the CoPt alloy with thickness was mitigated to have a near-ideal alloy composition of Co:Pt =80:20, which induces epitaxial-like growth from Ru substrate, thus resulting in the improvement of the crystal orientation of the hcp (002) structure from its initial deposition stages. Furthermore, the cross-sectional transmission electron microscope (TEM) analysis of the nanodots deposited with bath agitation showed CoPt growth along its c-axis oriented in the perpendicular direction, having uniform lattice fringes on the hcp (002) plane from the Ru underlayer interface, which is a significant factor to induce perpendicular magnetic anisotropy. Magnetic characterization of the CoPt nanodot arrays showed increase in the perpendicular coercivity and squareness of the hysteresis loops from 2.0 kOe and 0.64 (without agitation) to 4.0 kOe and 0.87 with bath agitation. Based on the detailed characterization of nanodot arrays, the precise crystal structure control of the nanodot arrays with ultra-high recording density by electrochemical process was successfully demonstrated.

Dual-mode acoustic wave biosensors microarrays

NASA Astrophysics Data System (ADS)

Auner, Gregory W.; Shreve, Gina; Ying, Hao; Newaz, Golam; Hughes, Chantelle; Xu, Jianzeng

2003-04-01

We have develop highly sensitive and selective acoustic wave biosensor arrays with signal analysis systems to provide a fingerprint for the real-time identification and quantification of a wide array of bacterial pathogens and environmental health hazards. We have developed an unique highly sensitive dual mode acoustic wave platform prototype that, when combined with phage based selective detection elements, form a durable bacteria sensor. Arrays of these new real-time biosensors are integrated to form a biosensor array on a chip. This research and development program optimizes advanced piezoelectric aluminum nitride wide bandgap semiconductors, novel micromachining processes, advanced device structures, selective phage displays development and immobilization techniques, and system integration and signal analysis technology to develop the biosensor arrays. The dual sensor platform can be programmed to sense in a gas, vapor or liquid environment by switching between acoustic wave resonate modes. Such a dual mode sensor has tremendous implications for applications involving monitoring of pathogenic microorganisms in the clinical setting due to their ability to detect airborne pathogens. This provides a number of applications including hospital settings such as intensive care or other in-patient wards for the reduction of nosocomial infections and maintenance of sterile environments in surgical suites. Monitoring for airborn pathogen transmission in public transportation areas such as airplanes may be useful for implementation of strategies for redution of airborn transmission routes. The ability to use the same sensor in the liquid sensing mode is important for tracing the source of airborn pathogens to local liquid sources. Sensing of pathogens in saliva will be useful for sensing oral pathogens and support of decision-making strategies regarding prevention of transmission and support of treatment strategies.

Phase Velocity and Full-Waveform Analysis of Co-located Distributed Acoustic Sensing (DAS) Channels and Geophone Sensor

NASA Astrophysics Data System (ADS)

Parker, L.; Mellors, R. J.; Thurber, C. H.; Wang, H. F.; Zeng, X.

2015-12-01

A 762-meter Distributed Acoustic Sensing (DAS) array with a channel spacing of one meter was deployed at the Garner Valley Downhole Array in Southern California. The array was approximately rectangular with dimensions of 180 meters by 80 meters. The array also included two subdiagonals within the rectangle along which three-component geophones were co-located. Several active sources were deployed, including a 45-kN, swept-frequency, shear-mass shaker, which produced strong Rayleigh waves across the array. Both DAS and geophone traces were filtered in 2-Hz steps between 4 and 20 Hz to obtain phase velocities as a function of frequency from fitting the moveout of travel times over distances of 35 meters or longer. As an alternative to this traditional means of finding phase velocity, it is theoretically possible to find the Rayleigh-wave phase velocity at each point of co-location as the ratio of DAS and geophone responses, because DAS is sensitive to ground strain and geophones are sensitive to ground velocity, after suitable corrections for instrument response (Mikumo & Aki, 1964). The concept was tested in WPP, a seismic wave propagation program, by first validating and then using a 3D synthetic, full-waveform seismic model to simulate the effect of increased levels of noise and uncertainty as data go from ideal to more realistic. The results obtained from this study provide a better understanding of the DAS response and its potential for being combined with traditional seismometers for obtaining phase velocity at a single location. This analysis is part of the PoroTomo project (Poroelastic Tomography by Adjoint Inverse Modeling of Data from Seismology, Geodesy, and Hydrology, http://geoscience.wisc.edu/feigl/porotomo).

High-density fiber optic biosensor arrays

NASA Astrophysics Data System (ADS)

Epstein, Jason R.; Walt, David R.

2002-02-01

Novel approaches are required to coordinate the immense amounts of information derived from diverse genomes. This concept has influenced the expanded role of high-throughput DNA detection and analysis in the biological sciences. A high-density fiber optic DNA biosensor was developed consisting of oligonucleotide-functionalized, 3.1 mm diameter microspheres deposited into the etched wells on the distal face of a 500 micrometers imaging fiber bundle. Imaging fiber bundles containing thousands of optical fibers, each associated with a unique oligonucleotide probe sequence, were the foundation for an optically connected, individually addressable DNA detection platform. Different oligonucleotide-functionalized microspheres were combined in a stock solution, and randomly dispersed into the etched wells. Microsphere positions were registered from optical dyes incorporated onto the microspheres. The distribution process provided an inherent redundancy that increases the signal-to-noise ratio as the square root of the number of sensors examined. The representative amount of each probe-type in the array was dependent on their initial stock solution concentration, and as other sequences of interest arise, new microsphere elements can be added to arrays without altering the existing detection capabilities. The oligonucleotide probe sequences hybridize to fluorescently-labeled, complementary DNA target solutions. Fiber optic DNA microarray research has included DNA-protein interaction profiles, microbial strain differentiation, non-labeled target interrogation with molecular beacons, and single cell-based assays. This biosensor array is proficient in DNA detection linked to specific disease states, single nucleotide polymorphism (SNP's) discrimination, and gene expression analysis. This array platform permits multiple detection formats, provides smaller feature sizes, and enables sensor design flexibility. High-density fiber optic microarray biosensors provide a fast, reversible format with the detection limit of a few hundred molecules.

Multi-Array Detection, Association and Location of Infrasound and Seismo-Acoustic Events in Utah

DTIC Science & Technology

2008-09-30

techniques for detecting , associating, and locating infrasound signals at single and multiple arrays and then combining the processed results with...was detected and located by both infrasound and seismic instruments (Figure 3). Infrasound signals at all three arrays , from one of the explosions, are...COVERED (From - To) 30-Sep-2008 REPRINT 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER MULTI- ARRAY DETECTION , ASSOCIATION AND LOCATION OF INFRASOUND FA8718

Performance analysis of a full-field and full-range swept-source OCT system

NASA Astrophysics Data System (ADS)

Krauter, J.; Boettcher, T.; Körner, K.; Gronle, M.; Osten, W.; Passilly, N.; Froehly, L.; Perrin, S.; Gorecki, C.

2015-09-01

In recent years, optical coherence tomography (OCT) became gained importance in medical disciplines like ophthalmology, due to its noninvasive optical imaging technique with micrometer resolution and short measurement time. It enables e. g. the measurement and visualization of the depth structure of the retina. In other medical disciplines like dermatology, histopathological analysis is still the gold standard for skin cancer diagnosis. The EU-funded project VIAMOS (Vertically Integrated Array-type Mirau-based OCT System) proposes a new type of OCT system combined with micro-technologies to provide a hand-held, low-cost and miniaturized OCT system. The concept is a combination of full-field and full-range swept-source OCT (SS-OCT) detection in a multi-channel sensor based on a micro-optical Mirau-interferometer array, which is fabricated by means of wafer fabrication. This paper presents the study of an experimental proof-of-concept OCT system as a one-channel sensor with bulk optics. This sensor is a Linnik-interferometer type with similar optical parameters as the Mirau-interferometer array. A commercial wavelength tunable light source with a center wavelength at 845nm and 50nm spectral bandwidth is used with a camera for parallel OCT A-Scan detection. In addition, the reference microscope objective lens of the Linnik-interferometer is mounted on a piezo-actuated phase-shifter. Phase-shifting interferometry (PSI) techniques are applied for resolving the conjugate complex artifact and consequently contribute to an increase of image quality and depth range. A suppression ratio of the complex conjugate term of 36 dB is shown and a system sensitivity greater than 96 dB could be measured.

Coil-to-coil physiological noise correlations and their impact on fMRI time-series SNR

PubMed Central

Triantafyllou, C.; Polimeni, J. R.; Keil, B.; Wald, L. L.

2017-01-01

Purpose Physiological nuisance fluctuations (“physiological noise”) are a major contribution to the time-series Signal to Noise Ratio (tSNR) of functional imaging. While thermal noise correlations between array coil elements have a well-characterized effect on the image Signal to Noise Ratio (SNR0), the element-to-element covariance matrix of the time-series fluctuations has not yet been analyzed. We examine this effect with a goal of ultimately improving the combination of multichannel array data. Theory and Methods We extend the theoretical relationship between tSNR and SNR0 to include a time-series noise covariance matrix Ψt, distinct from the thermal noise covariance matrix Ψ0, and compare its structure to Ψ0 and the signal coupling matrix SSH formed from the signal intensity vectors S. Results Inclusion of the measured time-series noise covariance matrix into the model relating tSNR and SNR0 improves the fit of experimental multichannel data and is shown to be distinct from Ψ0 or SSH. Conclusion Time-series noise covariances in array coils are found to differ from Ψ0 and more surprisingly, from the signal coupling matrix SSH. Correct characterization of the time-series noise has implications for the analysis of time-series data and for improving the coil element combination process. PMID:26756964

Open and scalable analytics of large Earth observation datasets: From scenes to multidimensional arrays using SciDB and GDAL

NASA Astrophysics Data System (ADS)

Appel, Marius; Lahn, Florian; Buytaert, Wouter; Pebesma, Edzer

2018-04-01

Earth observation (EO) datasets are commonly provided as collection of scenes, where individual scenes represent a temporal snapshot and cover a particular region on the Earth's surface. Using these data in complex spatiotemporal modeling becomes difficult as soon as data volumes exceed a certain capacity or analyses include many scenes, which may spatially overlap and may have been recorded at different dates. In order to facilitate analytics on large EO datasets, we combine and extend the geospatial data abstraction library (GDAL) and the array-based data management and analytics system SciDB. We present an approach to automatically convert collections of scenes to multidimensional arrays and use SciDB to scale computationally intensive analytics. We evaluate the approach in three study cases on national scale land use change monitoring with Landsat imagery, global empirical orthogonal function analysis of daily precipitation, and combining historical climate model projections with satellite-based observations. Results indicate that the approach can be used to represent various EO datasets and that analyses in SciDB scale well with available computational resources. To simplify analyses of higher-dimensional datasets as from climate model output, however, a generalization of the GDAL data model might be needed. All parts of this work have been implemented as open-source software and we discuss how this may facilitate open and reproducible EO analyses.

Parallel scheduling of recursively defined arrays

NASA Technical Reports Server (NTRS)

Myers, T. J.; Gokhale, M. B.

1986-01-01

A new method of automatic generation of concurrent programs which constructs arrays defined by sets of recursive equations is described. It is assumed that the time of computation of an array element is a linear combination of its indices, and integer programming is used to seek a succession of hyperplanes along which array elements can be computed concurrently. The method can be used to schedule equations involving variable length dependency vectors and mutually recursive arrays. Portions of the work reported here have been implemented in the PS automatic program generation system.

Preparation of a Superhydrophobic and Peroxidase-like Activity Array Chip for H2O2 Sensing by Surface-Enhanced Raman Scattering.

PubMed

Yu, Zhi; Park, Yeonju; Chen, Lei; Zhao, Bing; Jung, Young Mee; Cong, Qian

2015-10-28

In this paper, we propose a novel and simple method for preparing a dual-biomimetic functional array possessing both superhydrophobic and peroxidase-like activity that can be used for hydrogen peroxide (H2O2) sensing. The proposed method is an integration innovation that combines the above two properties and surface-enhanced Raman scattering (SERS). We integrated a series of well-ordered arrays of Au points (d = 1 mm) onto a superhydrophobic copper (Cu)/silver (Ag) surface by replicating an arrayed molybdenum template. Instead of using photoresists and the traditional lithography method, we utilized a chemical etching method (a substitution reaction between Cu and HAuCl4) with a Cu/Ag superhydrophobic surface as the barrier layer, which has the benefit of water repellency. The as-prepared Au points were observed to possess peroxidase-like activity, allowing for catalytic oxidation of the chromogenic molecule o-phenylenediamine dihydrochloride (OPD). Oxidation was evidenced by a color change in the presence of H2O2, which allows the array chip to act as an H2O2 sensor. In this study, the water repellency of the superhydrophobic surface was used to fabricate the array chip and increase the local reactant concentration during the catalytic reaction. As a result, the catalytic reaction occurred when only 2 μL of an aqueous sample (OPD/H2O2) was placed onto the Au point, and the enzymatic product, 2,3-diaminophenazine, showed a SERS signal distinguishable from that of OPD after mixing with 2 μL of colloidal Au. Using the dual-biomimetic functional array chip, quantitative analysis of H2O2 was performed by observing the change in the SERS spectra, which showed a concentration-dependent behavior for H2O2. This method allows for the detection of H2O2 at concentrations as low as 3 pmol per 2 μL of sample, which is a considerable advantage in H2O2 analysis. The as-prepared substrate was convenient for H2O2 detection because only a small amount of sample was required in each analysis. Highly sensitive detection was realized using SERS. Therefore, this chip was shown to exhibit significant potential for applications in bioanalysis.

Study of cross-shaped ultrasonic array sensor applied to partial discharge location in transformer oil.

PubMed

Li, Jisheng; Xin, Xiaohu; Luo, Yongfen; Ji, Haiying; Li, Yanming; Deng, Junbo

2013-11-01

A conformal combined sensor is designed and it is used in Partial Discharge (PD) location experiments in transformer oil. The sensor includes a cross-shaped ultrasonic phased array of 13 elements and an ultra-high-frequency (UHF) electromagnetic rectangle array of 2 × 2 elements. Virtual expansion with high order cumulants, the ultrasonic array can achieve the effect of array with 61 elements. This greatly improves the aperture and direction sharpness of original array and reduces the cost of follow-up hardware. With the cross-shaped ultrasonic array, the results of PD location experiments are precise and the maximum error of the direction of arrival (DOA) is less than 5°.

Study design and data analysis considerations for the discovery of prognostic molecular biomarkers: a case study of progression free survival in advanced serous ovarian cancer.

PubMed

Qin, Li-Xuan; Levine, Douglas A

2016-06-10

Accurate discovery of molecular biomarkers that are prognostic of a clinical outcome is an important yet challenging task, partly due to the combination of the typically weak genomic signal for a clinical outcome and the frequently strong noise due to microarray handling effects. Effective strategies to resolve this challenge are in dire need. We set out to assess the use of careful study design and data normalization for the discovery of prognostic molecular biomarkers. Taking progression free survival in advanced serous ovarian cancer as an example, we conducted empirical analysis on two sets of microRNA arrays for the same set of tumor samples: arrays in one set were collected using careful study design (that is, uniform handling and randomized array-to-sample assignment) and arrays in the other set were not. We found that (1) handling effects can confound the clinical outcome under study as a result of chance even with randomization, (2) the level of confounding handling effects can be reduced by data normalization, and (3) good study design cannot be replaced by post-hoc normalization. In addition, we provided a practical approach to define positive and negative control markers for detecting handling effects and assessing the performance of a normalization method. Our work showcased the difficulty of finding prognostic biomarkers for a clinical outcome of weak genomic signals, illustrated the benefits of careful study design and data normalization, and provided a practical approach to identify handling effects and select a beneficial normalization method. Our work calls for careful study design and data analysis for the discovery of robust and translatable molecular biomarkers.

Microstamped Petri Dishes for Scanning Electrochemical Microscopy Analysis of Arrays of Microtissues

PubMed Central

Sridhar, Adithya; de Boer, Hans L.; van den Berg, Albert; Le Gac, Séverine

2014-01-01

While scanning electrochemical microscopy (SECM) is a powerful technique for non-invasive analysis of cells, SECM-based assays remain scarce and have been mainly limited so far to single cells, which is mostly due to the absence of suitable platform for experimentation on 3D cellular aggregates or microtissues. Here, we report stamping of a Petri dish with a microwell array for large-scale production of microtissues followed by their in situ analysis using SECM. The platform is realized by hot embossing arrays of microwells (200 μm depth; 400 μm diameter) in commercially available Petri dishes, using a PDMS stamp. Microtissues form spontaneously in the microwells, which is demonstrated here using various cell lines (e.g., HeLa, C2C12, HepG2 and MCF-7). Next, the respiratory activity of live HeLa microtissues is assessed by monitoring the oxygen reduction current in constant height mode and at various distances above the platform surface. Typically, at a 40 μm distance from the microtissue, a 30% decrease in the oxygen reduction current is measured, while above 250 μm, no influence of the presence of the microtissues is detected. After exposure to a model drug (50% ethanol), no such changes in oxygen concentration are found at any height in solution, which reflects that microtissues are not viable anymore. This is furthermore confirmed using conventional live/dead fluorescent stains. This live/dead assay demonstrates the capability of the proposed approach combining SECM and microtissue arrays formed in a stamped Petri dish for conducting cellular assays in a non-invasive way on 3D cellular models. PMID:24690887

Test Structures For Bumpy Integrated Circuits

NASA Technical Reports Server (NTRS)

Buehler, Martin G.; Sayah, Hoshyar R.

1989-01-01

Cross-bridge resistors added to comb and serpentine patterns. Improved combination of test structures built into integrated circuit used to evaluate design rules, fabrication processes, and quality of interconnections. Consist of meshing serpentines and combs, and cross bridge. Structures used to make electrical measurements revealing defects in design or fabrication. Combination of test structures includes three comb arrays, two serpentine arrays, and cross bridge. Made of aluminum or polycrystalline silicon, depending on material in integrated-circuit layers evaluated. Aluminum combs and serpentine arrays deposited over steps made by polycrystalline silicon and diffusion layers, while polycrystalline silicon versions of these structures used to cross over steps made by thick oxide layer.

Primary gamma ray selection in a hybrid timing/imaging Cherenkov array

NASA Astrophysics Data System (ADS)

Postnikov, E. B.; Grinyuk, A. A.; Kuzmichev, L. A.; Sveshnikova, L. G.

2017-06-01

This work is a methodical study on hybrid reconstruction techniques for hybrid imaging/timing Cherenkov observations. This type of hybrid array is to be realized at the gamma-observatory TAIGA intended for very high energy gamma-ray astronomy (> 30 TeV). It aims at combining the cost-effective timing-array technique with imaging telescopes. Hybrid operation of both of these techniques can lead to a relatively cheap way of development of a large area array. The joint approach of gamma event selection was investigated on both types of simulated data: the image parameters from the telescopes, and the shower parameters reconstructed from the timing array. The optimal set of imaging parameters and shower parameters to be combined is revealed. The cosmic ray background suppression factor depending on distance and energy is calculated. The optimal selection technique leads to cosmic ray background suppression of about 2 orders of magnitude on distances up to 450 m for energies greater than 50 TeV.

Large gamma-ray detector arrays and electromagnetic separators

NASA Astrophysics Data System (ADS)

Lee, I.-Yang

2013-12-01

The use of large gamma-ray detector arrays with electromagnetic separators is a powerful combination. Various types of gamma-ray detectors have been used; some provide high detector efficiency such as scintillation detector array, others use Ge detectors for good energy resolution, and recently developed Ge energy tracking arrays gives both high peak-to-background ratio and position resolution. Similarly, different types of separators were used to optimize the performance under different experimental requirements and conditions. For example, gas-filled separators were used in heavy element studies for their large efficiency and beam rejection factor. Vacuum separators with good isotope resolution were used in transfer and fragmentation reactions for the study of nuclei far from stability. This paper presents results from recent experiments using gamma-ray detector arrays in combination with electromagnetic separators, and discusses the physics opportunities provided by these instruments. In particular, we review the performance of the instruments currently in use, and discuss the requirements of instruments for future radioactive beam accelerator facilities.

Location of high-frequency P wave microseismic noise in the Pacific Ocean using multiple small aperture arrays

DOE PAGES

Pyle, Moira L.; Koper, Keith D.; Euler, Garrett G.; ...

2015-04-20

We investigate source locations of P-wave microseisms within a narrow frequency band (0.67–1.33 Hz) that is significantly higher than the classic microseism band (~0.05–0.3 Hz). Employing a backprojection method, we analyze data recorded during January 2010 from five International Monitoring System arrays that border the Pacific Ocean. We develop a ranking scheme that allows us to combine beam power from multiple arrays to obtain robust locations of the microseisms. Some individual arrays exhibit a strong regional component, but results from the combination of all arrays show high-frequency P wave energy emanating from the North Pacific basin, in general agreement withmore » previous observations in the double-frequency (DF) microseism band (~0.1–0.3 Hz). This suggests that the North Pacific source of ambient P noise covers a broad range of frequencies and that the wave-wave interaction model is likely valid at shorter periods.« less

[Combined burn trauma in the array of modern civilian and combat burns].

PubMed

Ivchenko, E V; Borisov, D N; Golota, A S; Krassiĭ, A B; Rusev, I T

2015-02-01

The current article positions the combined burn and non-burn injuries in the general array of civilian and combat burns. For that purpose the official state statistics and scientific medical publications, domestic as well as foreign, have been analyzed. It has been shown that in peace time the combined burn/trauma injuries are infrequent. But the same type of injury becomes routine especially among the civilian population in the conditions of the modern so called "hybrid war". And the medical service should be prepared for it.

Model of experts for decision support in the diagnosis of leukemia patients.

PubMed

Corchado, Juan M; De Paz, Juan F; Rodríguez, Sara; Bajo, Javier

2009-07-01

Recent advances in the field of biomedicine, specifically in the field of genomics, have led to an increase in the information available for conducting expression analysis. Expression analysis is a technique used in transcriptomics, a branch of genomics that deals with the study of messenger ribonucleic acid (mRNA) and the extraction of information contained in the genes. This increase in information is reflected in the exon arrays, which require the use of new techniques in order to extract the information. The purpose of this study is to provide a tool based on a mixture of experts model that allows the analysis of the information contained in the exon arrays, from which automatic classifications for decision support in diagnoses of leukemia patients can be made. The proposed model integrates several cooperative algorithms characterized for their efficiency for data processing, filtering, classification and knowledge extraction. The Cancer Institute of the University of Salamanca is making an effort to develop tools to automate the evaluation of data and to facilitate de analysis of information. This proposal is a step forward in this direction and the first step toward the development of a mixture of experts tool that integrates different cognitive and statistical approaches to deal with the analysis of exon arrays. The mixture of experts model presented within this work provides great capacities for learning and adaptation to the characteristics of the problem in consideration, using novel algorithms in each of the stages of the analysis process that can be easily configured and combined, and provides results that notably improve those provided by the existing methods for exon arrays analysis. The material used consists of data from exon arrays provided by the Cancer Institute that contain samples from leukemia patients. The methodology used consists of a system based on a mixture of experts. Each one of the experts incorporates novel artificial intelligence techniques that improve the process of carrying out various tasks such as pre-processing, filtering, classification and extraction of knowledge. This article will detail the manner in which individual experts are combined so that together they generate a system capable of extracting knowledge, thus permitting patients to be classified in an automatic and efficient manner that is also comprehensible for medical personnel. The system has been tested in a real setting and has been used for classifying patients who suffer from different forms of leukemia at various stages. Personnel from the Cancer Institute supervised and participated throughout the testing period. Preliminary results are promising, notably improving the results obtained with previously used tools. The medical staff from the Cancer Institute considers the tools that have been developed to be positive and very useful in a supporting capacity for carrying out their daily tasks. Additionally the mixture of experts supplies a tool for the extraction of necessary information in order to explain the associations that have been made in simple terms. That is, it permits the extraction of knowledge for each classification made and generalized in order to be used in subsequent classifications. This allows for a large amount of learning and adaptation within the proposed system.

The Navy Precision Optical Interferometer: an update

NASA Astrophysics Data System (ADS)

Armstrong, J. T.; Baines, Ellyn K.; Schmitt, Henrique R.; Restaino, Sergio R.; Clark, James H.; Benson, James A.; Hutter, Donald J.; Zavala, Robert T.; van Belle, Gerard T.

2016-08-01

We describe the current status of the Navy Precision Optical Interferometer (NPOI), including developments since the last SPIE meeting. The NPOI group has added stations as far as 250m from the array center and added numerous infrastructure improvements. Science programs include stellar diameters and limb darkening, binary orbits, Be star disks, exoplanet host stars, and progress toward high-resolution stellar surface imaging. Technical and infrastructure projects include on-sky demonstrations of baseline bootstrapping with six array elements and of the VISION beam combiner, control system updates, integration of the long delay lines, and updated firmware for the Classic beam combiner. Our plans to add up to four 1.8 m telescopes are no longer viable, but we have recently acquired separate funding for adding three 1 m AO-equipped telescopes and an infrared beam combiner to the array.

Browsing Space Weather Data and Models with the Integrated Space Weather Analysis (iSWA) System

NASA Technical Reports Server (NTRS)

Maddox, Marlo M.; Mullinix, Richard E.; Berrios, David H.; Hesse, Michael; Rastaetter, Lutz; Pulkkinen, Antti; Hourcle, Joseph A.; Thompson, Barbara J.

2011-01-01

The Integrated Space Weather Analysis (iSWA) System is a comprehensive web-based platform for space weather information that combines data from solar, heliospheric and geospace observatories with forecasts based on the most advanced space weather models. The iSWA system collects, generates, and presents a wide array of space weather resources in an intuitive, user-configurable, and adaptable format - thus enabling users to respond to current and future space weather impacts as well as enabling post-impact analysis. iSWA currently provides over 200 data and modeling products, and features a variety of tools that allow the user to browse, combine, and examine data and models from various sources. This presentation will consist of a summary of the iSWA products and an overview of the customizable user interfaces, and will feature several tutorial demonstrations highlighting the interactive tools and advanced capabilities.

Combination solar photovoltaic heat engine energy converter

NASA Technical Reports Server (NTRS)

Chubb, Donald L.

1987-01-01

A combination solar photovoltaic heat engine converter is proposed. Such a system is suitable for either terrestrial or space power applications. The combination system has a higher efficiency than either the photovoltaic array or the heat engine alone can attain. Advantages in concentrator and radiator area and receiver mass of the photovoltaic heat engine system over a heat-engine-only system are estimated. A mass and area comparison between the proposed space station organic Rankine power system and a combination PV-heat engine system is made. The critical problem for the proposed converter is the necessity for high temperature photovoltaic array operation. Estimates of the required photovoltaic temperature are presented.

Erwinia amylovora CRISPR Elements Provide New Tools for Evaluating Strain Diversity and for Microbial Source Tracking

PubMed Central

McGhee, Gayle C.; Sundin, George W.

2012-01-01

Clustered regularly interspaced short palindromic repeats (CRISPRs) comprise a family of short DNA repeat sequences that are separated by non repetitive spacer sequences and, in combination with a suite of Cas proteins, are thought to function as an adaptive immune system against invading DNA. The number of CRISPR arrays in a bacterial chromosome is variable, and the content of each array can differ in both repeat number and in the presence or absence of specific spacers. We utilized a comparative sequence analysis of CRISPR arrays of the plant pathogen Erwinia amylovora to uncover previously unknown genetic diversity in this species. A total of 85 E. amylovora strains varying in geographic isolation (North America, Europe, New Zealand, and the Middle East), host range, plasmid content, and streptomycin sensitivity/resistance were evaluated for CRISPR array number and spacer variability. From these strains, 588 unique spacers were identified in the three CRISPR arrays present in E. amylovora, and these arrays could be categorized into 20, 17, and 2 patterns types, respectively. Analysis of the relatedness of spacer content differentiated most apple and pear strains isolated in the eastern U.S. from western U.S. strains. In addition, we identified North American strains that shared CRISPR genotypes with strains isolated on other continents. E. amylovora strains from Rubus and Indian hawthorn contained mostly unique spacers compared to apple and pear strains, while strains from loquat shared 79% of spacers with apple and pear strains. Approximately 23% of the spacers matched known sequences, with 16% targeting plasmids and 5% targeting bacteriophage. The plasmid pEU30, isolated in E. amylovora strains from the western U.S., was targeted by 55 spacers. Lastly, we used spacer patterns and content to determine that streptomycin-resistant strains of E. amylovora from Michigan were low in diversity and matched corresponding streptomycin-sensitive strains from the background population. PMID:22860008

Erwinia amylovora CRISPR elements provide new tools for evaluating strain diversity and for microbial source tracking.

PubMed

McGhee, Gayle C; Sundin, George W

2012-01-01

Clustered regularly interspaced short palindromic repeats (CRISPRs) comprise a family of short DNA repeat sequences that are separated by non repetitive spacer sequences and, in combination with a suite of Cas proteins, are thought to function as an adaptive immune system against invading DNA. The number of CRISPR arrays in a bacterial chromosome is variable, and the content of each array can differ in both repeat number and in the presence or absence of specific spacers. We utilized a comparative sequence analysis of CRISPR arrays of the plant pathogen Erwinia amylovora to uncover previously unknown genetic diversity in this species. A total of 85 E. amylovora strains varying in geographic isolation (North America, Europe, New Zealand, and the Middle East), host range, plasmid content, and streptomycin sensitivity/resistance were evaluated for CRISPR array number and spacer variability. From these strains, 588 unique spacers were identified in the three CRISPR arrays present in E. amylovora, and these arrays could be categorized into 20, 17, and 2 patterns types, respectively. Analysis of the relatedness of spacer content differentiated most apple and pear strains isolated in the eastern U.S. from western U.S. strains. In addition, we identified North American strains that shared CRISPR genotypes with strains isolated on other continents. E. amylovora strains from Rubus and Indian hawthorn contained mostly unique spacers compared to apple and pear strains, while strains from loquat shared 79% of spacers with apple and pear strains. Approximately 23% of the spacers matched known sequences, with 16% targeting plasmids and 5% targeting bacteriophage. The plasmid pEU30, isolated in E. amylovora strains from the western U.S., was targeted by 55 spacers. Lastly, we used spacer patterns and content to determine that streptomycin-resistant strains of E. amylovora from Michigan were low in diversity and matched corresponding streptomycin-sensitive strains from the background population.

Reduced-Drift Virtual Gyro from an Array of Low-Cost Gyros.

PubMed

Vaccaro, Richard J; Zaki, Ahmed S

2017-02-11

A Kalman filter approach for combining the outputs of an array of high-drift gyros to obtain a virtual lower-drift gyro has been known in the literature for more than a decade. The success of this approach depends on the correlations of the random drift components of the individual gyros. However, no method of estimating these correlations has appeared in the literature. This paper presents an algorithm for obtaining the statistical model for an array of gyros, including the cross-correlations of the individual random drift components. In order to obtain this model, a new statistic, called the "Allan covariance" between two gyros, is introduced. The gyro array model can be used to obtain the Kalman filter-based (KFB) virtual gyro. Instead, we consider a virtual gyro obtained by taking a linear combination of individual gyro outputs. The gyro array model is used to calculate the optimal coefficients, as well as to derive a formula for the drift of the resulting virtual gyro. The drift formula for the optimal linear combination (OLC) virtual gyro is identical to that previously derived for the KFB virtual gyro. Thus, a Kalman filter is not necessary to obtain a minimum drift virtual gyro. The theoretical results of this paper are demonstrated using simulated as well as experimental data. In experimental results with a 28-gyro array, the OLC virtual gyro has a drift spectral density 40 times smaller than that obtained by taking the average of the gyro signals.

Stabilized PV system

DOEpatents

Dinwoodie, Thomas L.

2002-12-17

A stabilized PV system comprises an array of photovoltaic (PV) assemblies mounted to a support surface. Each PV assembly comprises a PV module and a support assembly securing the PV module to a position overlying the support surface. The array of modules is circumscribed by a continuous, belt-like perimeter assembly. Cross strapping, extending above, below or through the array, or some combination of above, below and through the array, secures a first position along the perimeter assembly to at least a second position along the perimeter assembly thereby stabilizing the array against wind uplift forces. The first and second positions may be on opposite sides on the array.

High-Reliability Pump Module for Non-Planar Ring Oscillator Laser

NASA Technical Reports Server (NTRS)

Liu, Duncan T.; Qiu, Yueming; Wilson, Daniel W.; Dubovitsky, Serge; Forouhar, Siamak

2007-01-01

We propose and have demonstrated a prototype high-reliability pump module for pumping a Non-Planar Ring Oscillator (NPRO) laser suitable for space missions. The pump module consists of multiple fiber-coupled single-mode laser diodes and a fiber array micro-lens array based fiber combiner. The reported Single-Mode laser diode combiner laser pump module (LPM) provides a higher normalized brightness at the combined beam than multimode laser diode based LPMs. A higher brightness from the pump source is essential for efficient NPRO laser pumping and leads to higher reliability because higher efficiency requires a lower operating power for the laser diodes, which in turn increases the reliability and lifetime of the laser diodes. Single-mode laser diodes with Fiber Bragg Grating (FBG) stabilized wavelength permit the pump module to be operated without a thermal electric cooler (TEC) and this further improves the overall reliability of the pump module. The single-mode laser diode LPM is scalable in terms of the number of pump diodes and is capable of combining hundreds of fiber-coupled laser diodes. In the proof-of-concept demonstration, an e-beam written diffractive micro lens array, a custom fiber array, commercial 808nm single mode laser diodes, and a custom NPRO laser head are used. The reliability of the proposed LPM is discussed.

The NMR phased array.

PubMed

Roemer, P B; Edelstein, W A; Hayes, C E; Souza, S P; Mueller, O M

1990-11-01

We describe methods for simultaneously acquiring and subsequently combining data from a multitude of closely positioned NMR receiving coils. The approach is conceptually similar to phased array radar and ultrasound and hence we call our techniques the "NMR phased array." The NMR phased array offers the signal-to-noise ratio (SNR) and resolution of a small surface coil over fields-of-view (FOV) normally associated with body imaging with no increase in imaging time. The NMR phased array can be applied to both imaging and spectroscopy for all pulse sequences. The problematic interactions among nearby surface coils is eliminated (a) by overlapping adjacent coils to give zero mutual inductance, hence zero interaction, and (b) by attaching low input impedance preamplifiers to all coils, thus eliminating interference among next nearest and more distant neighbors. We derive an algorithm for combining the data from the phased array elements to yield an image with optimum SNR. Other techniques which are easier to implement at the cost of lower SNR are explored. Phased array imaging is demonstrated with high resolution (512 x 512, 48-cm FOV, and 32-cm FOV) spin-echo images of the thoracic and lumbar spine. Data were acquired from four-element linear spine arrays, the first made of 12-cm square coils and the second made of 8-cm square coils. When compared with images from a single 15 x 30-cm rectangular coil and identical imaging parameters, the phased array yields a 2X and 3X higher SNR at the depth of the spine (approximately 7 cm).

Nanostructured optical fibre arrays for high-density biochemical sensing and remote imaging.

PubMed

Deiss, F; Sojic, N; White, D J; Stoddart, P R

2010-01-01

Optical fibre bundles usually comprise a few thousand to tens of thousands of individually clad glass optical fibres. The ordered arrangement of the fibres enables coherent transmission of an image through the bundle and therefore enables analysis and viewing in remote locations. In fused bundles, this architecture has also been used to fabricate arrays of various micro to nano-scale surface structures (micro/nanowells, nanotips, triangles, etc.) over relatively large areas. These surface structures have been used to obtain new optical and analytical capabilities. Indeed, the imaging bundle can be thought of as a "starting material" that can be sculpted by a combination of fibre drawing and selective wet-chemical etching processes. A large variety of bioanalytical applications have thus been developed, ranging from nano-optics to DNA nanoarrays. For instance, nanostructured optical surfaces with intrinsic light-guiding properties have been exploited as surface-enhanced Raman scattering (SERS) platforms and as near-field probe arrays. They have also been productively associated with electrochemistry to fabricate arrays of transparent nanoelectrodes with electrochemiluminescent imaging properties. The confined geometry of the wells has been loaded with biosensing materials and used as femtolitre-sized vessels to detect single molecules. This review describes the fabrication of high-density nanostructured optical fibre arrays and summarizes the large range of optical and bioanalytical applications that have been developed, reflecting the versatility of this ordered light-guiding platform.

Synergism of Dewetting and Self-Wrinkling To Create Two-Dimensional Ordered Arrays of Functional Microspheres.

PubMed

Han, Xue; Hou, Jing; Xie, Jixun; Yin, Jian; Tong, Yi; Lu, Conghua; Möhwald, Helmuth

2016-06-29

Here we report a simple, novel, yet robust nonlithographic method for the controlled fabrication of two-dimensional (2-D) ordered arrays of polyethylene glycol (PEG) microspheres. It is based on the synergistic combination of two bottom-up processes enabling periodic structure formation for the first time: dewetting and the mechanical wrinkle formation. The deterministic dewetting results from the hydrophilic polymer PEG on an incompatible polystyrene (PS) film bound to a polydimethylsiloxane (PDMS) substrate, which is directed both by a wrinkled template and by the template-directed in-situ self-wrinkling PS/PDMS substrate. Two strategies have been introduced to achieve synergism to enhance the 2-D ordering, i.e., employing 2-D in-situ self-wrinkling substrates and boundary conditions. As a result, we achieve highly ordered 2-D arrays of PEG microspheres with desired self-organized microstructures, such as the array location (e.g., selectively on the crest/in the valley of the wrinkles), diameter, spacing of the microspheres, and array direction. Additionally, the coordination of PEG with HAuCl4 is utilized to fabricate 2-D ordered arrays of functional PEG-HAuCl4 composite microspheres, which are further converted into different Au nanoparticle arrays. This simple versatile combined strategy could be extended to fabricate highly ordered 2-D arrays of other functional materials and achieve desirable properties and functionalities.

Tissue Gene Expression Analysis Using Arrayed Normalized cDNA Libraries

PubMed Central

Eickhoff, Holger; Schuchhardt, Johannes; Ivanov, Igor; Meier-Ewert, Sebastian; O'Brien, John; Malik, Arif; Tandon, Neeraj; Wolski, Eryk-Witold; Rohlfs, Elke; Nyarsik, Lajos; Reinhardt, Richard; Nietfeld, Wilfried; Lehrach, Hans

2000-01-01

We have used oligonucleotide-fingerprinting data on 60,000 cDNA clones from two different mouse embryonic stages to establish a normalized cDNA clone set. The normalized set of 5,376 clones represents different clusters and therefore, in almost all cases, different genes. The inserts of the cDNA clones were amplified by PCR and spotted on glass slides. The resulting arrays were hybridized with mRNA probes prepared from six different adult mouse tissues. Expression profiles were analyzed by hierarchical clustering techniques. We have chosen radioactive detection because it combines robustness with sensitivity and allows the comparison of multiple normalized experiments. Sensitive detection combined with highly effective clustering algorithms allowed the identification of tissue-specific expression profiles and the detection of genes specifically expressed in the tissues investigated. The obtained results are publicly available (http://www.rzpd.de) and can be used by other researchers as a digital expression reference. [The sequence data described in this paper have been submitted to the EMBL data library under accession nos. AL360374–AL36537.] PMID:10958641

An integrated circuit with transmit beamforming flip-chip bonded to a 2-D CMUT array for 3-D ultrasound imaging.

PubMed

Wygant, Ira O; Jamal, Nafis S; Lee, Hyunjoo J; Nikoozadeh, Amin; Oralkan, Omer; Karaman, Mustafa; Khuri-Yakub, Butrus T

2009-10-01

State-of-the-art 3-D medical ultrasound imaging requires transmitting and receiving ultrasound using a 2-D array of ultrasound transducers with hundreds or thousands of elements. A tight combination of the transducer array with integrated circuitry eliminates bulky cables connecting the elements of the transducer array to a separate system of electronics. Furthermore, preamplifiers located close to the array can lead to improved receive sensitivity. A combined IC and transducer array can lead to a portable, high-performance, and inexpensive 3-D ultrasound imaging system. This paper presents an IC flip-chip bonded to a 16 x 16-element capacitive micromachined ultrasonic transducer (CMUT) array for 3-D ultrasound imaging. The IC includes a transmit beamformer that generates 25-V unipolar pulses with programmable focusing delays to 224 of the 256 transducer elements. One-shot circuits allow adjustment of the pulse widths for different ultrasound transducer center frequencies. For receiving reflected ultrasound signals, the IC uses the 32-elements along the array diagonals. The IC provides each receiving element with a low-noise 25-MHz-bandwidth transimpedance amplifier. Using a field-programmable gate array (FPGA) clocked at 100 MHz to operate the IC, the IC generated properly timed transmit pulses with 5-ns accuracy. With the IC flip-chip bonded to a CMUT array, we show that the IC can produce steered and focused ultrasound beams. We present 2-D and 3-D images of a wire phantom and 2-D orthogonal cross-sectional images (Bscans) of a latex heart phantom.

Thermal management of quantum cascade lasers in an individually addressable monolithic array architecture

NASA Astrophysics Data System (ADS)

Missaggia, Leo; Wang, Christine; Connors, Michael; Saar, Brian; Sanchez-Rubio, Antonio; Creedon, Kevin; Turner, George; Herzog, William

2016-03-01

There are a number of military and commercial applications for high-power laser systems in the mid-to-long-infrared wavelength range. By virtue of their demonstrated watt-level performance and wavelength diversity, quantum cascade laser (QCL) and amplifier devices are an excellent choice of emitter for those applications. To realize the power levels of interest, beam combining of arrays of these emitters is required and as a result, array technology must be developed. With this in mind, packaging and thermal management strategies were developed to facilitate the demonstration of a monolithic QCL array operating under CW conditions. Thermal models were constructed and simulations performed to determine the effect of parameters such as array-element ridge width and pitch on gain region temperature rise. The results of the simulations were considered in determining an appropriate QCL array configuration. State-of-the-art micro-impingement cooling along with an electrical distribution scheme comprised of AlN multi-layer technology were integrated into the design. The design of the module allows for individual electrical addressability of the array elements, a method of phase control demonstrated previously for coherent beam combining of diode arrays, along with access to both front and rear facets. Hence, both laser and single-pass amplifier arrays can be accommodated. A module was realized containing a 5 mm cavity length monolithic QCL array comprised of 7 elements on 450 m pitch. An output power of 3.16 W was demonstrated under CW conditions at an emission wavelength of 9μm.

M&A For Lithography Of Sparse Arrays Of Sub-Micrometer Features

DOEpatents

Brueck, Steven R.J.; Chen, Xiaolan; Zaidi, Saleem; Devine, Daniel J.

1998-06-02

Methods and apparatuses are disclosed for the exposure of sparse hole and/or mesa arrays with line:space ratios of 1:3 or greater and sub-micrometer hole and/or mesa diameters in a layer of photosensitive material atop a layered material. Methods disclosed include: double exposure interferometric lithography pairs in which only those areas near the overlapping maxima of each single-period exposure pair receive a clearing exposure dose; double interferometric lithography exposure pairs with additional processing steps to transfer the array from a first single-period interferometric lithography exposure pair into an intermediate mask layer and a second single-period interferometric lithography exposure to further select a subset of the first array of holes; a double exposure of a single period interferometric lithography exposure pair to define a dense array of sub-micrometer holes and an optical lithography exposure in which only those holes near maxima of both exposures receive a clearing exposure dose; combination of a single-period interferometric exposure pair, processing to transfer resulting dense array of sub-micrometer holes into an intermediate etch mask, and an optical lithography exposure to select a subset of initial array to form a sparse array; combination of an optical exposure, transfer of exposure pattern into an intermediate mask layer, and a single-period interferometric lithography exposure pair; three-beam interferometric exposure pairs to form sparse arrays of sub-micrometer holes; five- and four-beam interferometric exposures to form a sparse array of sub-micrometer holes in a single exposure. Apparatuses disclosed include arrangements for the three-beam, five-beam and four-beam interferometric exposures.

Lithographically patterned electrodeposition of gold, silver, and nickel nanoring arrays with widely tunable near-infrared plasmonic resonances.

PubMed

Halpern, Aaron R; Corn, Robert M

2013-02-26

A novel low-cost nanoring array fabrication method that combines the process of lithographically patterned nanoscale electrodeposition (LPNE) with colloidal lithography is described. Nanoring array fabrication was accomplished in three steps: (i) a thin (70 nm) sacrificial nickel or silver film was first vapor-deposited onto a plasma-etched packed colloidal monolayer; (ii) the polymer colloids were removed from the surface, a thin film of positive photoresist was applied, and a backside exposure of the photoresist was used to create a nanohole electrode array; (iii) this array of nanoscale cylindrical electrodes was then used for the electrodeposition of gold, silver, or nickel nanorings. Removal of the photoresist and sacrificial metal film yielded a nanoring array in which all of the nanoring dimensions were set independently: the inter-ring spacing was fixed by the colloidal radius, the radius of the nanorings was controlled by the plasma etching process, and the width of the nanorings was controlled by the electrodeposition process. A combination of scanning electron microscopy (SEM) measurements and Fourier transform near-infrared (FT-NIR) absorption spectroscopy were used to characterize the nanoring arrays. Nanoring arrays with radii from 200 to 400 nm exhibited a single strong NIR plasmonic resonance with an absorption maximum wavelength that varied linearly from 1.25 to 3.33 μm as predicted by a simple standing wave model linear antenna theory. This simple yet versatile nanoring array fabrication method was also used to electrodeposit concentric double gold nanoring arrays that exhibited multiple NIR plasmonic resonances.

Measurements and Analysis of Reverberation and Clutter Data

DTIC Science & Technology

2007-04-01

triplet arrays and the DRDC ar- ray with combined omnidirectional and dipole sensors. A fast shallow water reverberation model was extended to...Bistatic reverberation models are too slow for inversion, but model-data comparisons will be made using ray -based models, e.g. GSM [11], or normal-mode...July 2000, pp. 1183–1188, European Commission, Luxembourg. Meeting held at Lyon, France. [36] Weinberg, H. and Keenan, R. E. (1996), Gaussian ray

Economic analysis of the unified heliostat array

NASA Astrophysics Data System (ADS)

1981-01-01

Two heliostats, the Veda Industrial Heliostat (VIH) and the Repowering Heliostat were investigated in conjunction with the UHA. The UHA was found to be a viable candidate for solar thermal central receiver applications. The UHA-VIH combination was shown to provide very high flux densities and to be suitable for high temperature applications in the 10000 K to 20000 K range. These temperatures were shown to be achievable even with very small (1 MWt) collector fields.

Analyzing System on A Chip Single Event Upset Responses using Single Event Upset Data, Classical Reliability Models, and Space Environment Data

NASA Technical Reports Server (NTRS)

Berg, Melanie; LaBel, Kenneth; Campola, Michael; Xapsos, Michael

2017-01-01

We are investigating the application of classical reliability performance metrics combined with standard single event upset (SEU) analysis data. We expect to relate SEU behavior to system performance requirements. Our proposed methodology will provide better prediction of SEU responses in harsh radiation environments with confidence metrics. single event upset (SEU), single event effect (SEE), field programmable gate array devises (FPGAs)

Conventional and array-based comparative genomic hybridization analysis of nasopharyngeal carcinomas from the Mediterranean area.

PubMed

Rodriguez, S; Khabir, A; Keryer, C; Perrot, C; Drira, M; Ghorbel, A; Jlidi, R; Bernheim, A; Valent, A; Busson, P

2005-03-01

Nasopharyngeal carcinoma (NPC) occurs with a high incidence in Southeast Asia and to a lesser extent in the Mediterranean area, especially in Tunisia, Algeria, and Morocco. Cellular gene alterations combined with latent Epstein-Barr virus infection are thought to be essential for NPC oncogenesis. To date, chromosome analysis with comparative genomic hybridization (CGH) has been reported exclusively for NPCs from Southeast Asia. Although NPCs from the Mediterranean area have several distinct clinical and epidemiological features, CGH investigations have been lacking. Chromosome analysis was therefore undertaken on a series of NPC xenografts and biopsies derived from patients of Mediterranean origin. Four xenografts were investigated with a combination of conventional CGH, array-based CGH, and comparative expressed sequence hybridization. In addition, 23 fresh NPC biopsies were analyzed with conventional CGH. Data obtained from xenografts and fresh biopsies were consistent, except that amplification of genes at 18p was observed only in xenografts derived from metastatic tissues. Frequent gains associated with gene overexpression were detected at 1q25 approximately qter (64%) and 12p13 (50%). Losses were noticed mainly at 11q14 approximately q23 (50%), 13q12 approximately q31 (50%), 14q24 approximately q31 (43%), and 3p13 approximately p23 (43%). Comparison with previous reports suggests that Mediterranean NPCs have higher frequencies of gains at 1q and losses at 13q than their Asian counterparts.

C-2W Magnetic Measurement Suite

NASA Astrophysics Data System (ADS)

Roche, T.; Thompson, M. C.; Griswold, M.; Knapp, K.; Koop, B.; Ottaviano, A.; Tobin, M.; TAE, Tri Alpha Energy, Inc. Team

2017-10-01

Commissioning and early operations are underway on C-2W, Tri Alpha Energy's new FRC experiment. The increased complexity level of this machine requires an equally enhanced diagnostic capability. A fundamental component of any magnetically confined fusion experiment is a firm understanding of the magnetic field itself. C-2W is outfitted with over 700 magnetic field probes, 550 internal and 150 external. Innovative in-vacuum annular flux loop / B-dot combination probes will provide information about plasma shape, size, pressure, energy, total temperature, and trapped flux when coupled with establish theoretical interpretations. The massive Mirnov array, consisting of eight rings of eight 3D probes, will provide detailed information about plasma motion, stability, and MHD modal content with the aid of singular value decomposition (SVD) analysis. Internal Rogowski probes will detect the presence of axial currents flowing in the plasma jet in multiple axial locations. Initial data from this array of diagnostics will be presented along with some interpretation and discussion of the analysis techniques used.

A new multifunction acousto-optic signal processor

NASA Technical Reports Server (NTRS)

Berg, N. J.; Casseday, M. W.; Filipov, A. N.; Pellegrino, J. M.

1984-01-01

An acousto-optic architecture for simultaneously obtaining time integration correlation and high-speed power spectrum analysis was constructed using commercially available TeO2 modulators and photodiode detector-arrays. The correlator section of the processor uses coherent interferometry to attain maximum bandwidth and dynamic range while achieving a time-bandwidth product of 1 million. Two correllator outputs are achieved in this system configuration. One is optically filtered and magnified 2 : 1 to decrease the spatial frequency to a level where a 25-MHz bandwidth may be sampled by a 62-mm array with elements on 25-micro centers. The other output is magnified by a factor of 10 such that the center 4 microseconds of information is available for estimation of time-difference-of-arrival to within 10 ns. The Bragg cell spectrum-analyzer section, which also has two outputs, resolves a 25-MHz instantaneous bandwidth to 25 kHz and can determine discrete-frequency reception time to within 15 microseconds. A microprocessor combines spectrum analysis information with that obtained from the correlator.

High-throughput multiplex HLA-typing by ligase detection reaction (LDR) and universal array (UA) approach.

PubMed

Consolandi, Clarissa

2009-01-01

One major goal of genetic research is to understand the role of genetic variation in living systems. In humans, by far the most common type of such variation involves differences in single DNA nucleotides, and is thus termed single nucleotide polymorphism (SNP). The need for improvement in throughput and reliability of traditional techniques makes it necessary to develop new technologies. Thus the past few years have witnessed an extraordinary surge of interest in DNA microarray technology. This new technology offers the first great hope for providing a systematic way to explore the genome. It permits a very rapid analysis of thousands genes for the purpose of gene discovery, sequencing, mapping, expression, and polymorphism detection. We generated a series of analytical tools to address the manufacturing, detection and data analysis components of a microarray experiment. In particular, we set up a universal array approach in combination with a PCR-LDR (polymerase chain reaction-ligation detection reaction) strategy for allele identification in the HLA gene.

KASCADE-Grande observation of features in the cosmic ray spectrum between knee and ankle

NASA Astrophysics Data System (ADS)

Haungs, A.; Apel, W. D.; Arteaga-Velazquez, J. C.; Bekk, K.; Bertaina, M.; Blümer, J.; Bozdog, H.; Brancus, I. M.; Cantoni, E.; Chiavassa, A.; Cossavella, F.; Daumiller, K.; de Souza, V.; Di Pierro, F.; Doll, P.; Engel, R.; Engler, J.; Finger, M.; Fuchs, B.; Fuhrmann, D.; Gils, H. J.; Glasstetter, R.; Grupen, C.; Heck, D.; Hörandel, J. R.; Huber, D.; Huege, T.; Kampert, K.-H.; Kang, D.; Klages, H. O.; Link, K.; Łuczak, P.; Ludwig, M.; Mathes, H. J.; Mayer, H. J.; Melissas, M.; Milke, J.; Morello, C.; Oehlschläger, J.; Ostapchenko, S.; Palmieri, N.; Petcu, M.; Pierog, T.; Rebel, H.; Roth, M.; Schieler, H.; Schoo, S.; Schroder, F.; Sima, O.; Toma, G.; Trinchero, G. C.; Ulrich, H.; Weindl, A.; Wochele, J.; Wommer, M.; Zabierowski, J.

2013-02-01

The detection of high-energy cosmic rays above a few hundred TeV is realized by the observation of extensive air-showers. By using the multi-detector setup of KASCADE-Grande, and here in particular the detectors of the large Grande array, the energy spectrum and the elemental composition of high-energy cosmic rays in the energy range from 10 PeV to 1 EeV are investigated. The estimation of energy and mass of the high-energy primary particles is based on the combined analysis of the total number of charged particles and the total number of muons measured by the detector arrays of Grande and KASCADE, respectively. The latest analysis results have shown that in the all-particle spectrum two features are present: a hardening of the spectrum at energies around 20 PeV and a steepening, i.e. a knee-like structure, at 80-90 PeV. The latter one was found to be due to a decrease of flux of the heavy mass component.

Rate equation analysis and non-Hermiticity in coupled semiconductor laser arrays

NASA Astrophysics Data System (ADS)

Gao, Zihe; Johnson, Matthew T.; Choquette, Kent D.

2018-05-01

Optically coupled semiconductor laser arrays are described by coupled rate equations. The coupled mode equations and carrier densities are included in the analysis, which inherently incorporate the carrier-induced nonlinearities including gain saturation and amplitude-phase coupling. We solve the steady-state coupled rate equations and consider the cavity frequency detuning and the individual laser pump rates as the experimentally controlled variables. We show that the carrier-induced nonlinearities play a critical role in the mode control, and we identify gain contrast induced by cavity frequency detuning as a unique mechanism for mode control. Photon-mediated energy transfer between cavities is also discussed. Parity-time symmetry and exceptional points in this system are studied. Unbroken parity-time symmetry can be achieved by judiciously combining cavity detuning and unequal pump rates, while broken symmetry lies on the boundary of the optical locking region. Exceptional points are identified at the intersection between broken symmetry and unbroken parity-time symmetry.

A user-friendly workflow for analysis of Illumina gene expression bead array data available at the arrayanalysis.org portal.

PubMed

Eijssen, Lars M T; Goelela, Varshna S; Kelder, Thomas; Adriaens, Michiel E; Evelo, Chris T; Radonjic, Marijana

2015-06-30

Illumina whole-genome expression bead arrays are a widely used platform for transcriptomics. Most of the tools available for the analysis of the resulting data are not easily applicable by less experienced users. ArrayAnalysis.org provides researchers with an easy-to-use and comprehensive interface to the functionality of R and Bioconductor packages for microarray data analysis. As a modular open source project, it allows developers to contribute modules that provide support for additional types of data or extend workflows. To enable data analysis of Illumina bead arrays for a broad user community, we have developed a module for ArrayAnalysis.org that provides a free and user-friendly web interface for quality control and pre-processing for these arrays. This module can be used together with existing modules for statistical and pathway analysis to provide a full workflow for Illumina gene expression data analysis. The module accepts data exported from Illumina's GenomeStudio, and provides the user with quality control plots and normalized data. The outputs are directly linked to the existing statistics module of ArrayAnalysis.org, but can also be downloaded for further downstream analysis in third-party tools. The Illumina bead arrays analysis module is available at http://www.arrayanalysis.org . A user guide, a tutorial demonstrating the analysis of an example dataset, and R scripts are available. The module can be used as a starting point for statistical evaluation and pathway analysis provided on the website or to generate processed input data for a broad range of applications in life sciences research.

Quality assessment of crude and processed Arecae semen based on colorimeter and HPLC combined with chemometrics methods.

PubMed

Sun, Meng; Yan, Donghui; Yang, Xiaolu; Xue, Xingyang; Zhou, Sujuan; Liang, Shengwang; Wang, Shumei; Meng, Jiang

2017-05-01

Raw Arecae Semen, the seed of Areca catechu L., as well as Arecae Semen Tostum and Arecae semen carbonisata are traditionally processed by stir-baking for subsequent use in a variety of clinical applications. These three Arecae semen types, important Chinese herbal drugs, have been used in China and other Asian countries for thousands of years. In this study, the sensory technologies of a colorimeter and sensitive validated high-performance liquid chromatography with diode array detection were employed to discriminate raw Arecae semen and its processed drugs. The color parameters of the samples were determined by a colorimeter instrument CR-410. Moreover, the fingerprints of the four alkaloids of arecaidine, guvacine, arecoline and guvacoline were surveyed by high-performance liquid chromatography. Subsequently, Student's t test, the analysis of variance, fingerprint similarity analysis, hierarchical cluster analysis, principal component analysis, factor analysis and Pearson's correlation test were performed for final data analysis. The results obtained demonstrated a significant color change characteristic for components in raw Arecae semen and its processed drugs. Crude and processed Arecae semen could be determined based on colorimetry and high-performance liquid chromatography with a diode array detector coupled with chemometrics methods for a comprehensive quality evaluation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Digitally programmable microfluidic automaton for multiscale combinatorial mixing and sample processing†

PubMed Central

Jensen, Erik C.; Stockton, Amanda M.; Chiesl, Thomas N.; Kim, Jungkyu; Bera, Abhisek; Mathies, Richard A.

2013-01-01

A digitally programmable microfluidic Automaton consisting of a 2-dimensional array of pneumatically actuated microvalves is programmed to perform new multiscale mixing and sample processing operations. Large (µL-scale) volume processing operations are enabled by precise metering of multiple reagents within individual nL-scale valves followed by serial repetitive transfer to programmed locations in the array. A novel process exploiting new combining valve concepts is developed for continuous rapid and complete mixing of reagents in less than 800 ms. Mixing, transfer, storage, and rinsing operations are implemented combinatorially to achieve complex assay automation protocols. The practical utility of this technology is demonstrated by performing automated serial dilution for quantitative analysis as well as the first demonstration of on-chip fluorescent derivatization of biomarker targets (carboxylic acids) for microchip capillary electrophoresis on the Mars Organic Analyzer. A language is developed to describe how unit operations are combined to form a microfluidic program. Finally, this technology is used to develop a novel microfluidic 6-sample processor for combinatorial mixing of large sets (>26 unique combinations) of reagents. The digitally programmable microfluidic Automaton is a versatile programmable sample processor for a wide range of process volumes, for multiple samples, and for different types of analyses. PMID:23172232

Theory and design of compact hybrid microphone arrays on two-dimensional planes for three-dimensional soundfield analysis.

PubMed

Chen, Hanchi; Abhayapala, Thushara D; Zhang, Wen

2015-11-01

Soundfield analysis based on spherical harmonic decomposition has been widely used in various applications; however, a drawback is the three-dimensional geometry of the microphone arrays. In this paper, a method to design two-dimensional planar microphone arrays that are capable of capturing three-dimensional (3D) spatial soundfields is proposed. Through the utilization of both omni-directional and first order microphones, the proposed microphone array is capable of measuring soundfield components that are undetectable to conventional planar omni-directional microphone arrays, thus providing the same functionality as 3D arrays designed for the same purpose. Simulations show that the accuracy of the planar microphone array is comparable to traditional spherical microphone arrays. Due to its compact shape, the proposed microphone array greatly increases the feasibility of 3D soundfield analysis techniques in real-world applications.

An integrated bioinformatics approach to improve two-color microarray quality-control: impact on biological conclusions.

PubMed

van Haaften, Rachel I M; Luceri, Cristina; van Erk, Arie; Evelo, Chris T A

2009-06-01

Omics technology used for large-scale measurements of gene expression is rapidly evolving. This work pointed out the need of an extensive bioinformatics analyses for array quality assessment before and after gene expression clustering and pathway analysis. A study focused on the effect of red wine polyphenols on rat colon mucosa was used to test the impact of quality control and normalisation steps on the biological conclusions. The integration of data visualization, pathway analysis and clustering revealed an artifact problem that was solved with an adapted normalisation. We propose a possible point to point standard analysis procedure, based on a combination of clustering and data visualization for the analysis of microarray data.

Blood pressure loci identified with a gene-centric array.

PubMed

Johnson, Toby; Gaunt, Tom R; Newhouse, Stephen J; Padmanabhan, Sandosh; Tomaszewski, Maciej; Kumari, Meena; Morris, Richard W; Tzoulaki, Ioanna; O'Brien, Eoin T; Poulter, Neil R; Sever, Peter; Shields, Denis C; Thom, Simon; Wannamethee, Sasiwarang G; Whincup, Peter H; Brown, Morris J; Connell, John M; Dobson, Richard J; Howard, Philip J; Mein, Charles A; Onipinla, Abiodun; Shaw-Hawkins, Sue; Zhang, Yun; Davey Smith, George; Day, Ian N M; Lawlor, Debbie A; Goodall, Alison H; Fowkes, F Gerald; Abecasis, Gonçalo R; Elliott, Paul; Gateva, Vesela; Braund, Peter S; Burton, Paul R; Nelson, Christopher P; Tobin, Martin D; van der Harst, Pim; Glorioso, Nicola; Neuvrith, Hani; Salvi, Erika; Staessen, Jan A; Stucchi, Andrea; Devos, Nabila; Jeunemaitre, Xavier; Plouin, Pierre-François; Tichet, Jean; Juhanson, Peeter; Org, Elin; Putku, Margus; Sõber, Siim; Veldre, Gudrun; Viigimaa, Margus; Levinsson, Anna; Rosengren, Annika; Thelle, Dag S; Hastie, Claire E; Hedner, Thomas; Lee, Wai K; Melander, Olle; Wahlstrand, Björn; Hardy, Rebecca; Wong, Andrew; Cooper, Jackie A; Palmen, Jutta; Chen, Li; Stewart, Alexandre F R; Wells, George A; Westra, Harm-Jan; Wolfs, Marcel G M; Clarke, Robert; Franzosi, Maria Grazia; Goel, Anuj; Hamsten, Anders; Lathrop, Mark; Peden, John F; Seedorf, Udo; Watkins, Hugh; Ouwehand, Willem H; Sambrook, Jennifer; Stephens, Jonathan; Casas, Juan-Pablo; Drenos, Fotios; Holmes, Michael V; Kivimaki, Mika; Shah, Sonia; Shah, Tina; Talmud, Philippa J; Whittaker, John; Wallace, Chris; Delles, Christian; Laan, Maris; Kuh, Diana; Humphries, Steve E; Nyberg, Fredrik; Cusi, Daniele; Roberts, Robert; Newton-Cheh, Christopher; Franke, Lude; Stanton, Alice V; Dominiczak, Anna F; Farrall, Martin; Hingorani, Aroon D; Samani, Nilesh J; Caulfield, Mark J; Munroe, Patricia B

2011-12-09

Raised blood pressure (BP) is a major risk factor for cardiovascular disease. Previous studies have identified 47 distinct genetic variants robustly associated with BP, but collectively these explain only a few percent of the heritability for BP phenotypes. To find additional BP loci, we used a bespoke gene-centric array to genotype an independent discovery sample of 25,118 individuals that combined hypertensive case-control and general population samples. We followed up four SNPs associated with BP at our p < 8.56 × 10(-7) study-specific significance threshold and six suggestively associated SNPs in a further 59,349 individuals. We identified and replicated a SNP at LSP1/TNNT3, a SNP at MTHFR-NPPB independent (r(2) = 0.33) of previous reports, and replicated SNPs at AGT and ATP2B1 reported previously. An analysis of combined discovery and follow-up data identified SNPs significantly associated with BP at p < 8.56 × 10(-7) at four further loci (NPR3, HFE, NOS3, and SOX6). The high number of discoveries made with modest genotyping effort can be attributed to using a large-scale yet targeted genotyping array and to the development of a weighting scheme that maximized power when meta-analyzing results from samples ascertained with extreme phenotypes, in combination with results from nonascertained or population samples. Chromatin immunoprecipitation and transcript expression data highlight potential gene regulatory mechanisms at the MTHFR and NOS3 loci. These results provide candidates for further study to help dissect mechanisms affecting BP and highlight the utility of studying SNPs and samples that are independent of those studied previously even when the sample size is smaller than that in previous studies. Copyright © 2011 The American Society of Human Genetics. Published by Elsevier Inc. All rights reserved.

A comparison of machine learning and Bayesian modelling for molecular serotyping.

PubMed

Newton, Richard; Wernisch, Lorenz

2017-08-11

Streptococcus pneumoniae is a human pathogen that is a major cause of infant mortality. Identifying the pneumococcal serotype is an important step in monitoring the impact of vaccines used to protect against disease. Genomic microarrays provide an effective method for molecular serotyping. Previously we developed an empirical Bayesian model for the classification of serotypes from a molecular serotyping array. With only few samples available, a model driven approach was the only option. In the meanwhile, several thousand samples have been made available to us, providing an opportunity to investigate serotype classification by machine learning methods, which could complement the Bayesian model. We compare the performance of the original Bayesian model with two machine learning algorithms: Gradient Boosting Machines and Random Forests. We present our results as an example of a generic strategy whereby a preliminary probabilistic model is complemented or replaced by a machine learning classifier once enough data are available. Despite the availability of thousands of serotyping arrays, a problem encountered when applying machine learning methods is the lack of training data containing mixtures of serotypes; due to the large number of possible combinations. Most of the available training data comprises samples with only a single serotype. To overcome the lack of training data we implemented an iterative analysis, creating artificial training data of serotype mixtures by combining raw data from single serotype arrays. With the enhanced training set the machine learning algorithms out perform the original Bayesian model. However, for serotypes currently lacking sufficient training data the best performing implementation was a combination of the results of the Bayesian Model and the Gradient Boosting Machine. As well as being an effective method for classifying biological data, machine learning can also be used as an efficient method for revealing subtle biological insights, which we illustrate with an example.

Infrasound and seismic array analysis of snow avalanches: results from the 2015-2017 experiment in Dischma valley above Davos, Switzerland

NASA Astrophysics Data System (ADS)

Marchetti, Emanuele; van Herwijnen, Alec; Ripepe, Maurizio

2017-04-01

While flowing downhill a snow avalanche radiates seismic and infrasonic waves being coupled both with the ground and the atmosphere. Infrasound waves are mostly generated by the powder cloud of the avalanche, while seismic waves are mostly generated by the dense flowing snow mass on the ground, resulting in different energy partitioning between seismic and infrasound for different kinds of avalanches. This results into a general uncertainty on the efficiency of seismic and infrasound monitoring, in terms of the size and source-to-receiver distance of detectable events. Nevertheless, both seismic and infrasound have been used as monitoring systems for the remote detection of snow avalanches, being the reliable detection of snow avalanches of crucial importance to better understand triggering mechanisms, identify possible precursors, or improve avalanche forecasting. We present infrasonic and seismic array data collected during the winters of 2015- 2016 and 2016-2017 in the Dischma valley above Davos, Switzerland, where a five element infrasound array and a 7 element seismic array had been deployed at short distance from each other and with several avalanche paths nearby. Avalanche observation in the area is performed through automatic cameras providing additional information on the location, type (dry or wet), size and occurrence time of the avalanches released. The use of arrays instead of single sensors allows increasing the signal-to-noise ratio and identifying events in terms of back-azimuth and apparent velocity of the wave-field, thus providing indication on the source position of the recorded signal. For selected snow avalanches captured with automatic cameras, we therefore perform seismic and infrasound array processing to constrain the avalanche path and dynamics and investigate the partitioning of seismic and infrasound energy for the different portions of the avalanche path. Moreover we compare results of seismic and infrasound array processing for the whole 2015-2016 winter season in order to investigate the ability of the two monitoring systems to identify and characterize snow avalanches and the benefit of the combined seismo-acoustic analysis.

Ambient Vehicular Noise recorded on a 2D Distributed Fiber Optic Sensing Array :Applications to Permafrost Thaw Detection and Imaging

NASA Astrophysics Data System (ADS)

Ajo Franklin, J. B.; Lindsey, N.; Wagner, A. M.; Dou, S.; Martin, E. R.; Ekblaw, I.; Ulrich, C.; James, S. R.; Freifeld, B. M.; Daley, T. M.

2016-12-01

Distributed Acoustic Sensing (DAS) is a recently developed technique that allows the spatially dense ( 1m) continuous recording of seismic signals on long strands of commercial fiber optic cables. The availability of continuous recording on dense arrays offers unique possibilities for long-term timelapse monitoring of environmental processes in arctic environments. In the absence of a repeatable semi-permanent seismic source, the use of ambient surface wave noise from infrastructure use (e.g. moving vehicles) for seismic imaging allows tomographic monitoring of evolving subsurface systems. Challenges in such scenarios include (1) the processing requirements for dense (1000+ channel) arrays recording weeks to months of seismic data, (2) appropriate methods to retrieve empirical noise correlation functions (NCFs) in environments with non-optimal array geometries and both coherent as well as incoherent noise, and (3) semi-automated approaches to invert timelapse NCFs for near-surface soil properties.We present an exploratory study of data from a sparse 2D DAS array acquisition on 4000 linear meters of trenched fiber deployed in 10 crossing profiles. The dataset, collected during July and August of 2016, covers a zone of permafrost undergoing a controlled thaw induced by an array of resistive heaters. The site, located near a heavily used road, has a high level of infrastructure noise but exhibits distance-dependent variation in both noise amplitude and spectrum. We apply seismic interferometry to retrieve the empirical NCF across array subsections, and use collocated geophone and broadband sensors to measure the NCF against the true impulse response function of the medium. We demonstrate that the combination of vehicle tracking and data windowing allows improved reconstruction of stable NCFs appropriate for dispersion analysis and inversion. We also show both spatial and temporal patterns of background noise at the site using 2D beamforming and spectral analysis. Our results suggest that valuable information can be extracted from ambient noise recorded with DAS, particularly in the context of monitoring transformations in cold region environments.

The Contribution of Genetic Recombination to CRISPR Array Evolution

PubMed Central

Kupczok, Anne; Landan, Giddy; Dagan, Tal

2015-01-01

CRISPR (clustered regularly interspaced short palindromic repeats) is a microbial immune system against foreign DNA. Recognition sequences (spacers) encoded within the CRISPR array mediate the immune reaction in a sequence-specific manner. The known mechanisms for the evolution of CRISPR arrays include spacer acquisition from foreign DNA elements at the time of invasion and array erosion through spacer deletion. Here, we consider the contribution of genetic recombination between homologous CRISPR arrays to the evolution of spacer repertoire. Acquisition of spacers from exogenic arrays via recombination may confer the recipient with immunity against unencountered antagonists. For this purpose, we develop a novel method for the detection of recombination in CRISPR arrays by modeling the spacer order in arrays from multiple strains from the same species. Because the evolutionary signal of spacer recombination may be similar to that of pervasive spacer deletions or independent spacer acquisition, our method entails a robustness analysis of the recombination inference by a statistical comparison to resampled and perturbed data sets. We analyze CRISPR data sets from four bacterial species: two Gammaproteobacteria species harboring CRISPR type I and two Streptococcus species harboring CRISPR type II loci. We find that CRISPR array evolution in Escherichia coli and Streptococcus agalactiae can be explained solely by vertical inheritance and differential spacer deletion. In Pseudomonas aeruginosa, we find an excess of single spacers potentially incorporated into the CRISPR locus during independent acquisition events. In Streptococcus thermophilus, evidence for spacer acquisition by recombination is present in 5 out of 70 strains. Genetic recombination has been proposed to accelerate adaptation by combining beneficial mutations that arose in independent lineages. However, for most species under study, we find that CRISPR evolution is shaped mainly by spacer acquisition and loss rather than recombination. Since the evolution of spacer content is characterized by a rapid turnover, it is likely that recombination is not beneficial for improving phage resistance in the strains under study, or that it cannot be detected in the resolution of intraspecies comparisons. PMID:26085541

Vortex Generators in a Streamline-Traced, External-Compression Supersonic Inlet

NASA Technical Reports Server (NTRS)

Baydar, Ezgihan; Lu, Frank K.; Slater, John W.; Trefny, Charles J.

2017-01-01

Vortex generators within a streamline-traced, external-compression supersonic inlet for Mach 1.66 were investigated to determine their ability to increase total pressure recovery and reduce total pressure distortion. The vortex generators studied were rectangular vanes arranged in counter-rotating and co-rotating arrays. The vane geometric factors of interest included height, length, spacing, angle-of-incidence, and positions upstream and downstream of the inlet terminal shock. The flow through the inlet was simulated numerically through the solution of the steady-state, Reynolds-averaged Navier-Stokes equations on multi-block, structured grids using the Wind-US flow solver. The vanes were simulated using a vortex generator model. The inlet performance was characterized by the inlet total pressure recovery and the radial and circumferential total pressure distortion indices at the engine face. Design of experiments and statistical analysis methods were applied to quantify the effect of the geometric factors of the vanes and search for optimal vane arrays. Co-rotating vane arrays with negative angles-of-incidence positioned on the supersonic diffuser were effective in sweeping low-momentum flow from the top toward the sides of the subsonic diffuser. This distributed the low-momentum flow more evenly about the circumference of the subsonic diffuser and reduced distortion. Co-rotating vane arrays with negative angles-of-incidence or counter-rotating vane arrays positioned downstream of the terminal shock were effective in mixing higher-momentum flow with lower-momentum flow to increase recovery and decrease distortion. A strategy of combining a co-rotating vane array on the supersonic diffuser with a counter-rotating vane array on the subsonic diffuser was effective in increasing recovery and reducing distortion.

Reliability analysis method of a solar array by using fault tree analysis and fuzzy reasoning Petri net

NASA Astrophysics Data System (ADS)

Wu, Jianing; Yan, Shaoze; Xie, Liyang

2011-12-01

To address the impact of solar array anomalies, it is important to perform analysis of the solar array reliability. This paper establishes the fault tree analysis (FTA) and fuzzy reasoning Petri net (FRPN) models of a solar array mechanical system and analyzes reliability to find mechanisms of the solar array fault. The index final truth degree (FTD) and cosine matching function (CMF) are employed to resolve the issue of how to evaluate the importance and influence of different faults. So an improvement reliability analysis method is developed by means of the sorting of FTD and CMF. An example is analyzed using the proposed method. The analysis results show that harsh thermal environment and impact caused by particles in space are the most vital causes of the solar array fault. Furthermore, other fault modes and the corresponding improvement methods are discussed. The results reported in this paper could be useful for the spacecraft designers, particularly, in the process of redesigning the solar array and scheduling its reliability growth plan.

Rectenna array measurement results

NASA Technical Reports Server (NTRS)

Dickinson, R. M.

1980-01-01

The measured performance characteristics of a rectenna array are reviewed and compared to the performance of a single element. It is shown that the performance may be extrapolated from the individual element to that of the collection of elements. Techniques for current and voltage combining were demonstrated. The array performance as a function of various operating parameters is characterized and techniques for overvoltage protection and automatic fault clearing in the array demonstrated. A method for detecting failed elements also exists. Instrumentation for deriving performance effectiveness is described. Measured harmonic radiation patterns and fundamental frequency scattered patterns for a low level illumination rectenna array are presented.

Arrayed waveguide Sagnac interferometer.

PubMed

Capmany, José; Muñoz, Pascual; Sales, Salvador; Pastor, Daniel; Ortega, Beatriz; Martinez, Alfonso

2003-02-01

We present a novel device, an arrayed waveguide Sagnac interferometer, that combines the flexibility of arrayed waveguides and the wide application range of fiber or integrated optics Sagnac loops. We form the device by closing an array of wavelength-selective light paths provided by two arrayed waveguides with a single 2 x 2 coupler in a Sagnac configuration. The equations that describe the device's operation in general conditions are derived. A preliminary experimental demonstration is provided of a fiber prototype in passive operation that shows good agreement with the expected theoretical performance. Potential applications of the device in nonlinear operation are outlined and discussed.

Tutorial: Performance and reliability in redundant disk arrays

NASA Technical Reports Server (NTRS)

Gibson, Garth A.

1993-01-01

A disk array is a collection of physically small magnetic disks that is packaged as a single unit but operates in parallel. Disk arrays capitalize on the availability of small-diameter disks from a price-competitive market to provide the cost, volume, and capacity of current disk systems but many times their performance. Unfortunately, relative to current disk systems, the larger number of components in disk arrays leads to higher rates of failure. To tolerate failures, redundant disk arrays devote a fraction of their capacity to an encoding of their information. This redundant information enables the contents of a failed disk to be recovered from the contents of non-failed disks. The simplest and least expensive encoding for this redundancy, known as N+1 parity is highlighted. In addition to compensating for the higher failure rates of disk arrays, redundancy allows highly reliable secondary storage systems to be built much more cost-effectively than is now achieved in conventional duplicated disks. Disk arrays that combine redundancy with the parallelism of many small-diameter disks are often called Redundant Arrays of Inexpensive Disks (RAID). This combination promises improvements to both the performance and the reliability of secondary storage. For example, IBM's premier disk product, the IBM 3390, is compared to a redundant disk array constructed of 84 IBM 0661 3 1/2-inch disks. The redundant disk array has comparable or superior values for each of the metrics given and appears likely to cost less. In the first section of this tutorial, I explain how disk arrays exploit the emergence of high performance, small magnetic disks to provide cost-effective disk parallelism that combats the access and transfer gap problems. The flexibility of disk-array configurations benefits manufacturer and consumer alike. In contrast, I describe in this tutorial's second half how parallelism, achieved through increasing numbers of components, causes overall failure rates to rise. Redundant disk arrays overcome this threat to data reliability by ensuring that data remains available during and after component failures.

Compact multiwavelength transmitter module for multimode fiber optic ribbon cable

DOEpatents

Deri, Robert J.; Pocha, Michael D.; Larson, Michael C.; Garrett, Henry E.

2002-01-01

A compact multiwavelength transmitter module for multimode fiber optic ribbon cable, which couples light from an M.times.N array of emitters onto N fibers, where the M wavelength may be distributed across two or more vertical-cavity surface-emitting laser (VCSEL) chips, and combining emitters and multiplexer into a compact package that is compatible with placement on a printed circuit board. A key feature is bringing together two emitter arrays fabricated on different substrates--each array designed for a different wavelength--into close physical proximity. Another key feature is to compactly and efficiently combine the light from two or more clusters of optical emitters, each in a different wavelength band, into a fiber ribbon.

Oligonucleotide arrays vs. metaphase-comparative genomic hybridisation and BAC arrays for single-cell analysis: first applications to preimplantation genetic diagnosis for Robertsonian translocation carriers.

PubMed

Ramos, Laia; del Rey, Javier; Daina, Gemma; García-Aragonés, Manel; Armengol, Lluís; Fernandez-Encinas, Alba; Parriego, Mònica; Boada, Montserrat; Martinez-Passarell, Olga; Martorell, Maria Rosa; Casagran, Oriol; Benet, Jordi; Navarro, Joaquima

2014-01-01

Comprehensive chromosome analysis techniques such as metaphase-Comparative Genomic Hybridisation (CGH) and array-CGH are available for single-cell analysis. However, while metaphase-CGH and BAC array-CGH have been widely used for Preimplantation Genetic Diagnosis, oligonucleotide array-CGH has not been used in an extensive way. A comparison between oligonucleotide array-CGH and metaphase-CGH has been performed analysing 15 single fibroblasts from aneuploid cell-lines and 18 single blastomeres from human cleavage-stage embryos. Afterwards, oligonucleotide array-CGH and BAC array-CGH were also compared analysing 16 single blastomeres from human cleavage-stage embryos. All three comprehensive analysis techniques provided broadly similar cytogenetic profiles; however, non-identical profiles appeared when extensive aneuploidies were present in a cell. Both array techniques provided an optimised analysis procedure and a higher resolution than metaphase-CGH. Moreover, oligonucleotide array-CGH was able to define extra segmental imbalances in 14.7% of the blastomeres and it better determined the specific unbalanced chromosome regions due to a higher resolution of the technique (≈ 20 kb). Applicability of oligonucleotide array-CGH for Preimplantation Genetic Diagnosis has been demonstrated in two cases of Robertsonian translocation carriers 45,XY,der(13;14)(q10;q10). Transfer of euploid embryos was performed in both cases and pregnancy was achieved by one of the couples. This is the first time that an oligonucleotide array-CGH approach has been successfully applied to Preimplantation Genetic Diagnosis for balanced chromosome rearrangement carriers.

Oligonucleotide Arrays vs. Metaphase-Comparative Genomic Hybridisation and BAC Arrays for Single-Cell Analysis: First Applications to Preimplantation Genetic Diagnosis for Robertsonian Translocation Carriers

PubMed Central

Ramos, Laia; del Rey, Javier; Daina, Gemma; García-Aragonés, Manel; Armengol, Lluís; Fernandez-Encinas, Alba; Parriego, Mònica; Boada, Montserrat; Martinez-Passarell, Olga; Martorell, Maria Rosa; Casagran, Oriol; Benet, Jordi; Navarro, Joaquima

2014-01-01

Comprehensive chromosome analysis techniques such as metaphase-Comparative Genomic Hybridisation (CGH) and array-CGH are available for single-cell analysis. However, while metaphase-CGH and BAC array-CGH have been widely used for Preimplantation Genetic Diagnosis, oligonucleotide array-CGH has not been used in an extensive way. A comparison between oligonucleotide array-CGH and metaphase-CGH has been performed analysing 15 single fibroblasts from aneuploid cell-lines and 18 single blastomeres from human cleavage-stage embryos. Afterwards, oligonucleotide array-CGH and BAC array-CGH were also compared analysing 16 single blastomeres from human cleavage-stage embryos. All three comprehensive analysis techniques provided broadly similar cytogenetic profiles; however, non-identical profiles appeared when extensive aneuploidies were present in a cell. Both array techniques provided an optimised analysis procedure and a higher resolution than metaphase-CGH. Moreover, oligonucleotide array-CGH was able to define extra segmental imbalances in 14.7% of the blastomeres and it better determined the specific unbalanced chromosome regions due to a higher resolution of the technique (≈20 kb). Applicability of oligonucleotide array-CGH for Preimplantation Genetic Diagnosis has been demonstrated in two cases of Robertsonian translocation carriers 45,XY,der(13;14)(q10;q10). Transfer of euploid embryos was performed in both cases and pregnancy was achieved by one of the couples. This is the first time that an oligonucleotide array-CGH approach has been successfully applied to Preimplantation Genetic Diagnosis for balanced chromosome rearrangement carriers. PMID:25415307

SOSPAC- SOLAR SPACE POWER ANALYSIS CODE

NASA Technical Reports Server (NTRS)

Selcuk, M. K.

1994-01-01

The Solar Space Power Analysis Code, SOSPAC, was developed to examine the solar thermal and photovoltaic power generation options available for a satellite or spacecraft in low earth orbit. SOSPAC is a preliminary systems analysis tool and enables the engineer to compare the areas, weights, and costs of several candidate electric and thermal power systems. The configurations studied include photovoltaic arrays and parabolic dish systems to produce electricity only, and in various combinations to provide both thermal and electric power. SOSPAC has been used for comparison and parametric studies of proposed power systems for the NASA Space Station. The initial requirements are projected to be about 40 kW of electrical power, and a similar amount of thermal power with temperatures above 1000 degrees Centigrade. For objects in low earth orbit, the aerodynamic drag caused by suitably large photovoltaic arrays is very substantial. Smaller parabolic dishes can provide thermal energy at a collection efficiency of about 80%, but at increased cost. SOSPAC allows an analysis of cost and performance factors of five hybrid power generating systems. Input includes electrical and thermal power requirements, sun and shade durations for the satellite, and unit weight and cost for subsystems and components. Performance equations of the five configurations are derived, and the output tabulates total weights of the power plant assemblies, area of the arrays, efficiencies, and costs. SOSPAC is written in FORTRAN IV for batch execution and has been implemented on an IBM PC computer operating under DOS with a central memory requirement of approximately 60K of 8 bit bytes. This program was developed in 1985.

Analysis of the seismic wavefield in the Moesian Platform (Bucharest area) for hazard assessment purposes

NASA Astrophysics Data System (ADS)

Manea, Elena Florinela; Michel, Clotaire; Hobiger, Manuel; Fäh, Donat; Cioflan, Carmen Ortanza; Radulian, Mircea

2017-09-01

During large earthquakes generated at intermediate depth in the Vrancea seismic zone, the ground motion recorded in Bucharest (Romania) is characterized by predominant long periods with strong amplification. Time-frequency analysis highlights the generation of low frequency surface waves (<1 Hz) for sufficiently strong and superficial events. This phenomenon has been explained by the influence of both source mechanism (radiation pattern, directivity effects) and mechanical properties of the local geological structure (geological layering and geometry). The main goal of our study is to better characterize and understand the seismic wavefield produced by earthquakes in the area of Bucharest, taking into account its location in the centre of the Moesian Platform, a large sedimentary basin (450 km long, 300 km wide and up to 20 km deep). To this aim, we identify the contribution of different seismic surface waves, such as the ones produced at the edges of this large sedimentary basin or multipath interference waves (Airy phases of Love and Rayleigh waves), on ground motion. The data from a 35 km diameter array (URS experiment) were used. The array was installed by the National Institute for Earth Physics in cooperation with the Karlsruhe Institute for Technology and operated during 10 months in 2003 and 2004 in the urban area of Bucharest and adjacent zones. The earthquake wavefield recorded by the URS array was analysed using the MUSIQUE technique. This technique analyses the three-component signals of all sensors of a seismic array together. The analysis includes 19 earthquakes with epicentral distances from 100 to 1560 km and with various backazimuths with enough energy at low frequencies (0.1-1 Hz), within the resolution range of the array. For all events, the largest portion of energy is arriving from the source direction and the wavefield is dominated by Love waves. The results of the array analyses clearly indicate a significant scattering corresponding to 2-D or 3-D effects of the Moesian Platform. The azimuthal distribution shows that the scattering comes primarily from the southern and northern edges of the basin. The Airy phase of Love waves was clearly identified as the main contributor in the range of the fundamental frequency of resonance of the basin (0.15-0.25 Hz), with directionality along the backazimuth and its opposite direction. Moreover, two further distinct frequency bands around 0.4 and 0.7 Hz with higher amplitudes were identified. Their complex nature is a combination of the higher modes of Rayleigh waves, Airy phases of Love waves and SH waves. Love and Rayleigh wave dispersion curves were successfully retrieved by combining the information of all events and show a good match with the ones obtained using ambient vibrations. Additionally, the first higher mode of Rayleigh waves could be retrieved using data from earthquakes. Also, the prograde and retrograde Rayleigh wave ellipticity was computed.

A digital combining-weight estimation algorithm for broadband sources with the array feed compensation system

NASA Technical Reports Server (NTRS)

Vilnrotter, V. A.; Rodemich, E. R.

1994-01-01

An algorithm for estimating the optimum combining weights for the Ka-band (33.7-GHz) array feed compensation system was developed and analyzed. The input signal is assumed to be broadband radiation of thermal origin, generated by a distant radio source. Currently, seven video converters operating in conjunction with the real-time correlator are used to obtain these weight estimates. The algorithm described here requires only simple operations that can be implemented on a PC-based combining system, greatly reducing the amount of hardware. Therefore, system reliability and portability will be improved.

Performance characteristics of a combination solar photovoltaic heat engine energy converter

NASA Technical Reports Server (NTRS)

Chubb, Donald L.

1987-01-01

A combination solar photovoltaic heat engine converter is proposed. Such a system is suitable for either terrestrial or space power applications. The combination system has a higher efficiency than either the photovoltaic array or the heat engine alone can attain. Advantages in concentrator and radiator area and receiver mass of the photovoltaic heat engine system over a heat-engine-only system are estimated. A mass and area comparison between the proposed space station organic Rankine power system and a combination PV-heat engine system is made. The critical problem for the proposed converter is the necessity for high temperature photovoltaic array operation. Estimates of the required photovoltaic temperature are presented.

Large Phased Array Radar Using Networked Small Parabolic Reflectors

NASA Technical Reports Server (NTRS)

Amoozegar, Farid

2006-01-01

Multifunction phased array systems with radar, telecom, and imaging applications have already been established for flat plate phased arrays of dipoles, or waveguides. In this paper the design trades and candidate options for combining the radar and telecom functions of the Deep Space Network (DSN) into a single large transmit array of small parabolic reflectors will be discussed. In particular the effect of combing the radar and telecom functions on the sizes of individual antenna apertures and the corresponding spacing between the antenna elements of the array will be analyzed. A heterogeneous architecture for the DSN large transmit array is proposed to meet the radar and telecom requirements while considering the budget, scheduling, and strategic planning constrains.

The CHARA Array Database

NASA Astrophysics Data System (ADS)

Jones, Jeremy; Schaefer, Gail; ten Brummelaar, Theo; Gies, Douglas; Farrington, Christopher

2018-01-01

We are building a searchable database for the CHARA Array data archive. The Array consists of six telescopes linked together as an interferometer, providing sub-milliarcsecond resolution in the optical and near-infrared. The Array enables a variety of scientific studies, including measuring stellar angular diameters, imaging stellar shapes and surface features, mapping the orbits of close binary companions, and resolving circumstellar environments. This database is one component of an NSF/MSIP funded program to provide open access to the CHARA Array to the broader astronomical community. This archive goes back to 2004 and covers all the beam combiners on the Array. We discuss the current status of and future plans for the public database, and give directions on how to access it.

Design of Hybrid Nanostructural Arrays to Manipulate SERS-Active Substrates by Nanosphere Lithography.

PubMed

Zhao, Xiaoyu; Wen, Jiahong; Zhang, Mengning; Wang, Dunhui; Wang, Yaxin; Chen, Lei; Zhang, Yongjun; Yang, Jinghai; Du, Youwei

2017-03-01

An easy-handling and low-cost method is utilized to controllably fabricate nanopattern arrays as the surface-enhanced Raman scattering (SERS) active substrates with high density of SERS-active areas (hot spots). A hybrid silver array of nanocaps and nanotriangles are prepared by combining magnetron sputtering and plasma etching. By adjusting the etching time of polystyrene (PS) colloid spheres array in silver nanobowls, the morphology of the arrays can be easily manipulated to control the formation and distribution of hot spots. The experimental results show that the hybrid nanostructural arrays have large enhancement factor, which is estimated to be seven times larger than that in the array of nanocaps and three times larger than that in the array of nanorings and nanoparticles. According to the results of finite-difference time-domain simulation, the excellent SERS performance of this array is ascribed to the high density of hot spots and enhanced electromagnetic field.

KASCADE-Grande: Composition studies in the view of the post-LHC hadronic interaction models

NASA Astrophysics Data System (ADS)

Haungs, A.; Apel, W. D.; Arteaga-Velázquez, J. C.; Bekk, K.; Bertaina, M.; Blümer, J.; Bozdog, H.; Brancus, I. M.; Cantoni, E.; Chiavassa, A.; Cossavella, F.; Daumiller, K.; de Souza, V.; Pierro, F. Di; Doll, P.; Engel, R.; Fuhrmann, D.; Gherghel-Lascu, A.; Gils, H. J.; Glasstetter, R.; Grupen, C.; Heck, D.; Hörandel, J. R.; Huege, T.; Kampert, K.-H.; Kang, D.; Klages, H. O.; Link, K.; Łuczak, P.; Mathes, H. J.; Mayer, H. J.; Milke, J.; Mitrica, B.; Morello, C.; Oehlschläger, J.; Ostapchenko, S.; Pierog, T.; Rebel, H.; Roth, M.; Schieler, H.; Schoo, S.; Schröder, F. G.; Sima, O.; Toma, G.; Trinchero, G. C.; Ulrich, H.; Weindl, A.; Wochele, J.; Zabierowski, J.

2017-06-01

The KASCADE-Grande experiment has significantly contributed to the current knowledge about the energy spectrum and composition of cosmic rays for energies between the knee and the ankle. Meanwhile, post-LHC versions of the hadronic interaction models are available and used to interpret the entire data set of KASCADE-Grande. In addition, a new, combined analysis of both arrays, KASCADE and Grande, was developed significantly increasing the accuracy of the shower observables. First results of the new analysis with the entire data set of the KASCADE-Grande experiment will be the focus of this contribution.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Casperson, R. J.; Burke, J. T.; Hughes, R. O.

Directly measuring (n,2n) cross sections on short-lived actinides presents a number of experimental challenges. The surrogate reaction technique is an experimental method for measuring cross sections on short-­lived isotopes, and it provides a unique solution for measuring (n,2n) cross sections. This technique involves measuring a charged-­particle reaction cross section, where the reaction populates the same compound nucleus as the reaction of interest. To perform these surrogate (n,2n) cross section measurements, a silicon telescope array has been placed along a beam line at the Texas A&M University Cyclotron Institute, which is surrounded by a large tank of gadolinium-doped liquid scintillator, whichmore » acts as a neutron detector. The combination of the charge-particle and neutron-detector arrays is referred to as NeutronSTARS. In the analysis procedure for calculating the (n,2n) cross section, the neutron detection efficiency and time structure plays an important role. Due to the lack of availability of isotropic, mono-energetic neutron sources, modeling is an important component in establishing this efficiency and time structure. This report describes the NeutronSTARS array, which was designed and commissioned during this project. It also describes the surrogate reaction technique, specifically referencing a 235U(n,2n) commissioning measurement that was fielded during the past year. Advanced multiplicity analysis techniques have been developed for this work, which should allow for efficient analysis of 241Pu(n,2n) and 239Pu(n,2n) cross section measurements« less

A photovoltaic catenary-tent array for the Martian surface

NASA Technical Reports Server (NTRS)

Crutchik, M.; Colozza, Anthony J.; Appelbaum, J.

1993-01-01

To provide electrical power during an exploration mission to Mars, a deployable tent-shaped structure with a flexible photovoltaic (PV) blanket is proposed. The array is designed with a self-deploying mechanism utilizing pressurized gas expansion. The structural design for the array uses a combination of cables, beams, and columns to support and deploy the PV blanket. Under the force of gravity a cable carrying a uniform load will take the shape of a catenary curve. A catenary-tent collector is self shadowing which must be taken into account in the solar radiation calculation. The shape and the area of the shadow on the array was calculated and used in the determination of the global radiation on the array. The PV blanket shape and structure dimension were optimized to achieve a configuration which maximizes the specific power (W/kg). The optimization was performed for four types of PV blankets (Si, GaAs/Ge, GaAs CLEFT, and amorphous Si) and four types of structure materials (Carbon composite, Aramid Fiber composite, Aluminum, and Magnesium). The results show that the catenary shape of the PV blanket, which produces the highest specific power, corresponds to zero end angle at the base with respect to the horizontal. The tent angle is determined by the combined effect of the array structure specific mass and the PV blanket output power. The combination of carbon composite structural material and GaAs CLEFT solar cells produce the highest specific power. The study was carried out for two sites on Mars corresponding to the Viking Lander locations. The designs were also compared for summer, winter, and yearly operation.

Gene ARMADA: an integrated multi-analysis platform for microarray data implemented in MATLAB.

PubMed

Chatziioannou, Aristotelis; Moulos, Panagiotis; Kolisis, Fragiskos N

2009-10-27

The microarray data analysis realm is ever growing through the development of various tools, open source and commercial. However there is absence of predefined rational algorithmic analysis workflows or batch standardized processing to incorporate all steps, from raw data import up to the derivation of significantly differentially expressed gene lists. This absence obfuscates the analytical procedure and obstructs the massive comparative processing of genomic microarray datasets. Moreover, the solutions provided, heavily depend on the programming skills of the user, whereas in the case of GUI embedded solutions, they do not provide direct support of various raw image analysis formats or a versatile and simultaneously flexible combination of signal processing methods. We describe here Gene ARMADA (Automated Robust MicroArray Data Analysis), a MATLAB implemented platform with a Graphical User Interface. This suite integrates all steps of microarray data analysis including automated data import, noise correction and filtering, normalization, statistical selection of differentially expressed genes, clustering, classification and annotation. In its current version, Gene ARMADA fully supports 2 coloured cDNA and Affymetrix oligonucleotide arrays, plus custom arrays for which experimental details are given in tabular form (Excel spreadsheet, comma separated values, tab-delimited text formats). It also supports the analysis of already processed results through its versatile import editor. Besides being fully automated, Gene ARMADA incorporates numerous functionalities of the Statistics and Bioinformatics Toolboxes of MATLAB. In addition, it provides numerous visualization and exploration tools plus customizable export data formats for seamless integration by other analysis tools or MATLAB, for further processing. Gene ARMADA requires MATLAB 7.4 (R2007a) or higher and is also distributed as a stand-alone application with MATLAB Component Runtime. Gene ARMADA provides a highly adaptable, integrative, yet flexible tool which can be used for automated quality control, analysis, annotation and visualization of microarray data, constituting a starting point for further data interpretation and integration with numerous other tools.

Fabrication and characterization of multi-stopband Fabry-Pérot filter array for nanospectrometers in the VIS range using SCIL nanoimprint technology

NASA Astrophysics Data System (ADS)

Shen, Yannan; Istock, André; Zaman, Anik; Woidt, Carsten; Hillmer, Hartmut

2018-05-01

Miniaturization of optical spectrometers can be achieved by Fabry-Pérot (FP) filter arrays. Each FP filter consists of two parallel highly reflecting mirrors and a resonance cavity in between. Originating from different individual cavity heights, each filter transmits a narrow spectral band (transmission line) with different wavelengths. Considering the fabrication efficiency, plasma enhanced chemical vapor deposition (PECVD) technology is applied to implement the high-optical-quality distributed Bragg reflectors (DBRs), while substrate conformal imprint lithography (one type of nanoimprint technology) is utilized to achieve the multiple cavities in just a single step. The FP filter array fabricated by nanoimprint combined with corresponding detector array builds a so-called "nanospectrometer". However, the silicon nitride and silicon dioxide stacks deposited by PECVD result in a limited stopband width of DBR (i.e., < 100 nm), which then limits the sensing range of filter arrays. However, an extension of the spectral range of filter arrays is desired and the topic of this investigation. In this work, multiple DBRs with different central wavelengths (λ c) are structured, deposited, and combined on a single substrate to enlarge the entire stopband. Cavity arrays are successfully aligned and imprinted over such terrace like surface in a single step. With this method, small chip size of filter arrays can be preserved, and the fabrication procedure of multiple resonance cavities is kept efficient as well. The detecting range of filter arrays is increased from roughly 50 nm with single DBR to 163 nm with three different DBRs.

6-shogaol induces apoptosis and enhances radiosensitivity in head and neck squamous cell carcinoma cell lines.

PubMed

Kotowski, Ulana; Kadletz, Lorenz; Schneider, Sven; Foki, Elisabeth; Schmid, Rainer; Seemann, Rudolf; Thurnher, Dietmar; Heiduschka, Gregor

2018-02-01

Ginger (Zingiber officinale Roscoe) is used for a wide array of conditions in traditional medicine in Asia, but little is known about the effect on head and neck cancer. In this study, the effect of two major pharmacologically active compounds of ginger, 6-gingerol and 6-shogaol, were studied on head and neck cancer cell lines. Furthermore, experiments in combination with established treatment methods for head and neck cancer were performed. Proliferation assays showed a dose-dependent reduction of cell viability. Flow cytometry analysis revealed the induction of apoptosis. Western blot analysis indicated that the antiapoptotic protein survivin was suppressed after treatment. Although a combination of 6-shogaol with cisplatin exhibited no synergistic effect, the combination with irradiation showed a synergistic reduction of clonogenic survival. In conclusion, ginger compounds have many noteworthy effects on head and neck cancer cell lines. In particular, the enhancement of radiosensitivity is remarkable. Copyright © 2017 John Wiley & Sons, Ltd.

Exome-chip association analysis reveals an Asian-specific missense variant in PAX4 associated with type 2 diabetes in Chinese individuals.

PubMed

Cheung, Chloe Y Y; Tang, Clara S; Xu, Aimin; Lee, Chi-Ho; Au, Ka-Wing; Xu, Lin; Fong, Carol H Y; Kwok, Kelvin H M; Chow, Wing-Sun; Woo, Yu-Cho; Yuen, Michele M A; Hai, JoJo S H; Jin, Ya-Li; Cheung, Bernard M Y; Tan, Kathryn C B; Cherny, Stacey S; Zhu, Feng; Zhu, Tong; Thomas, G Neil; Cheng, Kar-Keung; Jiang, Chao-Qiang; Lam, Tai-Hing; Tse, Hung-Fat; Sham, Pak-Chung; Lam, Karen S L

2017-01-01

Genome-wide association studies (GWASs) have identified many common type 2 diabetes-associated variants, mostly at the intronic or intergenic regions. Recent advancements of exome-array genotyping platforms have opened up a novel means for detecting the associations of low-frequency or rare coding variants with type 2 diabetes. We conducted an exomechip association analysis to identify additional type 2 diabetes susceptibility variants in the Chinese population. An exome-chip association study was conducted by genotyping 5640 Chinese individuals from Hong Kong, using a custom designed exome array, the Asian Exomechip. Single variant association analysis was conducted on 77,468 single nucleotide polymorphisms (SNPs). Fifteen SNPs were subsequently genotyped for replication analysis in an independent Chinese cohort comprising 12,362 individuals from Guangzhou. A combined analysis involving 7189 cases and 10,813 controls was performed. In the discovery stage, an Asian-specific coding variant rs2233580 (p.Arg192His) in PAX4, and two variants at the known loci, CDKN2B-AS1 and KCNQ1, were significantly associated with type 2 diabetes with exome-wide significance (p discovery  < 6.45 × 10 -7 ). The risk allele (T) of PAX4 rs2233580 was associated with a younger age at diabetes diagnosis. This variant was replicated in an independent cohort and demonstrated a stronger association that reached genome-wide significance (p meta-analysis [p meta ] = 3.74 × 10 -15 ) in the combined analysis. We identified the association of a PAX4 Asian-specific missense variant rs2233580 with type 2 diabetes in an exome-chip association analysis, supporting the involvement of PAX4 in the pathogenesis of type 2 diabetes. Our findings suggest PAX4 is a possible effector gene of the 7q32 locus, previously identified from GWAS in Asians.

Energy spectrum measured by the telescope array surface detector

NASA Astrophysics Data System (ADS)

Ivanov, Dmitri

2012-05-01

Two conflicting measurements of the ultra high energy cosmic ray (UHECR) flux have been reported by the Akeno Giant Air Shower Array (AGASA) and the High Resolution Fly's Eye (HiRes) experiments. HiRes observes a ˜5sigma suppression at E = 1019.75 eV, which is in agreement with the prediction of Greisen-Zatsepin-Kuz'min (GZK) theory. AGASA, in contrast, sees the flux extended well beyond E = 1020 eV with no visible break, suggesting that the flux is limited only by the rate at which the sources can produce the UHECR and not by interaction of energetic particles with the cosmic microwave background, thus challenging the relativistic invariance principle. In response to this discrepancy, a new experiment named the Telescope Array (TA) has been deployed, which combines the detection elements used separately by HiRes and AGASA. We describe the TA surface detector (SD) analysis using a technique new to the field, which consists of a detailed Monte-Carlo (MC) simulation of the SD response to the natural cosmic rays, validating the MC by comparing its distributions with the data, and calculation of the SD aperture from the MC. We will also describe our reconstruction procedure, based solely upon the data, and its application to both data and the MC. Finally, we will describe the energy spectrum resulting from this analysis, which is found to be in excellent agreement with the HiRes result, and as such, is the first confirmation of the GZK effect by a ground array of scintillation counters.

Experimental implementation of array-compressed parallel transmission at 7 tesla.

PubMed

Yan, Xinqiang; Cao, Zhipeng; Grissom, William A

2016-06-01

To implement and validate a hardware-based array-compressed parallel transmission (acpTx) system. In array-compressed parallel transmission, a small number of transmit channels drive a larger number of transmit coils, which are connected via an array compression network that implements optimized coil-to-channel combinations. A two channel-to-eight coil array compression network was developed using power splitters, attenuators and phase shifters, and a simulation was performed to investigate the effects of coil coupling on power dissipation in a simplified network. An eight coil transmit array was constructed using induced current elimination decoupling, and the coil and network were validated in benchtop measurements, B1+ mapping scans, and an accelerated spiral excitation experiment. The developed attenuators came within 0.08 dB of the desired attenuations, and reflection coefficients were -22 dB or better. The simulation demonstrated that up to 3× more power was dissipated in the network when coils were poorly isolated (-9.6 dB), versus well-isolated (-31 dB). Compared to split circularly-polarized coil combinations, the additional degrees of freedom provided by the array compression network led to 54% lower squared excitation error in the spiral experiment. Array-compressed parallel transmission was successfully implemented in a hardware system. Further work is needed to develop remote network tuning and to minimize network power dissipation. Magn Reson Med 75:2545-2552, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Fabrication of spherical microlens array by combining lapping on silicon wafer and rapid surface molding

NASA Astrophysics Data System (ADS)

Liu, Xiaohua; Zhou, Tianfeng; Zhang, Lin; Zhou, Wenchen; Yu, Jianfeng; Lee, L. James; Yi, Allen Y.

2018-07-01

Silicon is a promising mold material for compression molding because of its properties of hardness and abrasion resistance. Silicon wafers with carbide-bonded graphene coating and micro-patterns were evaluated as molds for the fabrication of microlens arrays. This study presents an efficient but flexible manufacturing method for microlens arrays that combines a lapping method and a rapid molding procedure. Unlike conventional processes for microstructures on silicon wafers, such as diamond machining and photolithography, this research demonstrates a unique approach by employing precision steel balls and diamond slurries to create microlenses with accurate geometry. The feasibility of this method was demonstrated by the fabrication of several microlens arrays with different aperture sizes and pitches on silicon molds. The geometrical accuracy and surface roughness of the microlens arrays were measured using an optical profiler. The measurement results indicated good agreement with the optical profile of the design. The silicon molds were then used to copy the microstructures onto polymer substrates. The uniformity and quality of the samples molded through rapid surface molding were also assessed and statistically quantified. To further evaluate the optical functionality of the molded microlens arrays, the focal lengths of the microlens arrays were measured using a simple optical setup. The measurements showed that the microlens arrays molded in this research were compatible with conventional manufacturing methods. This research demonstrated an alternative low-cost and efficient method for microstructure fabrication on silicon wafers, together with the follow-up optical molding processes.

Adaptive Locally Optimum Processing for Interference Suppression from Communication and Undersea Surveillance Signals

DTIC Science & Technology

1994-07-01

1993. "Analysis of the 1730-1732. Track - Before - Detect Approach to Target Detection using Pixel Statistics", to appear in IEEE Transactions Scholz, J...large surveillance arrays. One approach to combining energy in different spatial cells is track - before - detect . References to examples appear in the next... track - before - detect problem. The results obtained are not expected to depend strongly on model details. In particular, the structure of the tracking

Hierarchically structured Co₃O₄@Pt@MnO₂ nanowire arrays for high-performance supercapacitors.

PubMed

Xia, Hui; Zhu, Dongdong; Luo, Zhentao; Yu, Yue; Shi, Xiaoqin; Yuan, Guoliang; Xie, Jianping

2013-10-17

Here we proposed a novel architectural design of a ternary MnO2-based electrode - a hierarchical Co3O4@Pt@MnO2 core-shell-shell structure, where the complemental features of the three key components (a well-defined Co3O4 nanowire array on the conductive Ti substrate, an ultrathin layer of small Pt nanoparticles, and a thin layer of MnO2 nanoflakes) are strategically combined into a single entity to synergize and construct a high-performance electrode for supercapacitors. Owing to the high conductivity of the well-defined Co3O4 nanowire arrays, in which the conductivity was further enhanced by a thin metal (Pt) coating layer, in combination with the large surface area provided by the small MnO2 nanoflakes, the as-fabricated Co3O4@Pt@MnO2 nanowire arrays have exhibited high specific capacitances, good rate capability, and excellent cycling stability. The architectural design demonstrated in this study provides a new approach to fabricate high-performance MnO2-based nanowire arrays for constructing next-generation supercapacitors.

Hierarchically Structured Co3O4@Pt@MnO2 Nanowire Arrays for High-Performance Supercapacitors

NASA Astrophysics Data System (ADS)

Xia, Hui; Zhu, Dongdong; Luo, Zhentao; Yu, Yue; Shi, Xiaoqin; Yuan, Guoliang; Xie, Jianping

2013-10-01

Here we proposed a novel architectural design of a ternary MnO2-based electrode - a hierarchical Co3O4@Pt@MnO2 core-shell-shell structure, where the complemental features of the three key components (a well-defined Co3O4 nanowire array on the conductive Ti substrate, an ultrathin layer of small Pt nanoparticles, and a thin layer of MnO2 nanoflakes) are strategically combined into a single entity to synergize and construct a high-performance electrode for supercapacitors. Owing to the high conductivity of the well-defined Co3O4 nanowire arrays, in which the conductivity was further enhanced by a thin metal (Pt) coating layer, in combination with the large surface area provided by the small MnO2 nanoflakes, the as-fabricated Co3O4@Pt@MnO2 nanowire arrays have exhibited high specific capacitances, good rate capability, and excellent cycling stability. The architectural design demonstrated in this study provides a new approach to fabricate high-performance MnO2-based nanowire arrays for constructing next-generation supercapacitors.

Imaging 2015 Mw 7.8 Gorkha Earthquake and Its Aftershock Sequence Combining Multiple Calibrated Global Seismic Arrays

NASA Astrophysics Data System (ADS)

LI, B.; Ghosh, A.

2016-12-01

The 2015 Mw 7.8 Gorkha earthquake provides a good opportunity to study the tectonics and earthquake hazards in the Himalayas, one of the most seismically active plate boundaries. Details of the seismicity patterns and associated structures in the Himalayas are poorly understood mainly due to limited instrumentation. Here, we apply a back-projection method to study the mainshock rupture and the following aftershock sequence using four large aperture global seismic arrays. All the arrays show eastward rupture propagation of about 130 km and reveal similar evolution of seismic energy radiation, with strong high-frequency energy burst about 50 km north of Kathmandu. Each single array, however, is typically limited by large azimuthal gap, low resolution, and artifacts due to unmodeled velocity structures. Therefore, we use a self-consistent empirical calibration method to combine four different arrays to image the Gorkha event. It greatly improves the resolution, can better track rupture and reveal details that cannot be resolved by any individual array. In addition, we also use the same arrays at teleseismic distances and apply a back-projection technique to detect and locate the aftershocks immediately following the Gorkha earthquake. We detect about 2.5 times the aftershocks recorded by the Advance National Seismic System comprehensive earthquake catalog during the 19 days following the mainshock. The aftershocks detected by the arrays show an east-west trend in general, with majority of the aftershocks located at the eastern part of the rupture patch and surrounding the rupture zone of the largest Mw 7.3 aftershock. Overall spatiotemporal aftershock pattern agrees well with global catalog, with our catalog showing more details relative to the standard global catalog. The improved aftershock catalog enables us to better study the aftershock dynamics, stress evolution in this region. Moreover, rapid and better imaging of aftershock distribution may aid rapid response and hazard assessment after destructive large earthquakes. Existing multiple global seismic arrays, when properly calibrated and used in combinations, provide a high resolution image of rupture of large earthquakes and spatiotemporal distribution of aftershocks.

Power System Mass Analysis for Hydrogen Reduction Oxygen Production on the Lunar Surface

NASA Technical Reports Server (NTRS)

Colozza, Anthony J.

2009-01-01

The production of oxygen from the lunar regolith requires both thermal and electrical power in roughly similar proportions. This unique power requirement is unlike most applications on the lunar surface. To efficiently meet these requirements, both solar PV array and solar concentrator systems were evaluated. The mass of various types of photovoltaic and concentrator based systems were calculated to determine the type of power system that provided the highest specific power. These were compared over a range of oxygen production rates. Also a hybrid type power system was also considered. This system utilized a photovoltaic array to produce the electrical power and a concentrator to provide the thermal power. For a single source system the three systems with the highest specific power were a flexible concentrator/Stirling engine system, a rigid concentrator/Stirling engine system and a tracking triple junction solar array system. These systems had specific power values of 43, 34, and 33 W/kg, respectively. The hybrid power system provided much higher specific power values then the single source systems. The best hybrid combinations were the triple junction solar array with the flexible concentrator and the rigid concentrator. These systems had a specific power of 81 and 68 W/kg, respectively.

Editorial

NASA Astrophysics Data System (ADS)

Buitink, S.; Hörandel, J. R.; de Jong, S.; Lahmann, R.; Nahnhauer, R.; Scholten, O.

2017-03-01

This proceeding gives a summary of the current status and open questions of the radio technique for cosmic-ray air showers, assuming that the reader is already familiar with the principles. It includes recent results of selected experiments not present at this conference, e.g., LOPES and TREND. Current radio arrays like AERA or Tunka-Rex have demonstrated that areas of several km2 can be instrumented for reasonable costs with antenna spacings of the order of 200m. For the energy of the primary particle such sparse antenna arrays can already compete in absolute accuracy with other precise techniques, like the detection of air-fluorescence or air-Cherenkov light. With further improvements in the antenna calibration, the radio detection might become even more accurate. For the atmospheric depth of the shower maximum, Xmax, currently only the dense array LOFAR features a precision similar to the fluorescence technique, but analysis methods for the radio measurement of Xmax are still under development. Moreover, the combination of radio and muon measurements is expected to increase the accuracy of the mass composition, and this around-the-clock recording is not limited to clear nights as are the light-detection methods. Consequently, radio antennas will be a valuable add-on for any air shower array targeting the energy range above 100 PeV.

Inclined dislocation arrays in AlGaN/AlGaN quantum well structures emitting at 290 nm

NASA Astrophysics Data System (ADS)

Chang, T. Y.; Moram, M. A.; McAleese, C.; Kappers, M. J.; Humphreys, C. J.

2010-12-01

We report on the structural and optical properties of deep ultraviolet emitting AlGaN/AlGaN multiple quantum wells (MQWs) grown on (0001) sapphire by metal-organic vapor phase epitaxy using two different buffer layer structures, one containing a thin (1 μm) AlN layer combined with a GaN interlayer and the other a thick (4 μm) AlN layer. Transmission electron microscopy analysis of both structures showed inclined arrays of dislocations running through the AlGaN layers at an angle of ˜30°, originating at bunched steps at the AlN surface and terminating at bunched steps at the surface of the MQW structure. In all layers, these inclined dislocation arrays are surrounded by AlGaN with a relatively higher Ga content, consistent with plan-view cathodoluminescence maps in which the bunched surface steps are associated with longer emission wavelengths. The structure with the 4 μm-thick AlN buffer layer had a dislocation density lower by a factor of 2 (at (1.7±0.1)×109 cm-2) compared to the structure with the 1 μm thick AlN buffer layer, despite the presence of the inclined dislocation arrays.

Phased array antenna matching: Simulation and optimization of a planar phased array of circular waveguide elements

NASA Technical Reports Server (NTRS)

Dudgeon, J. E.

1972-01-01

A computerized simulation of a planar phased array of circular waveguide elements is reported using mutual coupling and wide angle impedance matching in phased arrays. Special emphasis is given to circular polarization. The aforementioned computer program has as variable inputs: frequency, polarization, grid geometry, element size, dielectric waveguide fill, dielectric plugs in the waveguide for impedance matching, and dielectric sheets covering the array surface for the purpose of wide angle impedance matching. Parameter combinations are found which produce reflection peaks interior to grating lobes, while dielectric cover sheets are successfully employed to extend the usable scan range of a phased array. The most exciting results came from the application of computer aided optimization techniques to the design of this type of array.

Global Arrays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Krishnamoorthy, Sriram; Daily, Jeffrey A.; Vishnu, Abhinav

2015-11-01

Global Arrays (GA) is a distributed-memory programming model that allows for shared-memory-style programming combined with one-sided communication, to create a set of tools that combine high performance with ease-of-use. GA exposes a relatively straightforward programming abstraction, while supporting fully-distributed data structures, locality of reference, and high-performance communication. GA was originally formulated in the early 1990’s to provide a communication layer for the Northwest Chemistry (NWChem) suite of chemistry modeling codes that was being developed concurrently.

Analytical modelling of Halbach linear generator incorporating pole shifting and piece-wise spring for ocean wave energy harvesting

NASA Astrophysics Data System (ADS)

Tan, Yimin; Lin, Kejian; Zu, Jean W.

2018-05-01

Halbach permanent magnet (PM) array has attracted tremendous research attention in the development of electromagnetic generators for its unique properties. This paper has proposed a generalized analytical model for linear generators. The slotted stator pole-shifting and implementation of Halbach array have been combined for the first time. Initially, the magnetization components of the Halbach array have been determined using Fourier decomposition. Then, based on the magnetic scalar potential method, the magnetic field distribution has been derived employing specially treated boundary conditions. FEM analysis has been conducted to verify the analytical model. A slotted linear PM generator with Halbach PM has been constructed to validate the model and further improved using piece-wise springs to trigger full range reciprocating motion. A dynamic model has been developed to characterize the dynamic behavior of the slider. This analytical method provides an effective tool in development and optimization of Halbach PM generator. The experimental results indicate that piece-wise springs can be employed to improve generator performance under low excitation frequency.

Fabrication and Theoretical Evaluation of Microlens Arrays on Layered Polymers

NASA Astrophysics Data System (ADS)

Oder, Tom; McMaster, Michael; Merlo, Corey; Bagheri, Camron; Reakes, Clayton; Petrus, Joshua; Li, Dingqiang; Crescimanno, Michael; Andrews, James

2014-03-01

Arrays of microlens were fabricated on nano-layered polymers using reactive ion etching. Semi hemispherical patterns with diameters ranging from 20 to 80 micrometers were first formed on a thick photoresist film that was spin-coated on the layered polymers using standard photolithographic process employing a gray scale glass mask. These patterns were then transferred to the polymers using dry etching in a reactive ion etching system. The optimized etch condition included a mixture of sulfur hexafluoride and oxygen, which resulted in an etch depth of 5 micrometers and successfully exposed the individual sub-micron thick layers in the polymers. Physical characterization of the microlens arrays was done using atomic force microscope and scanning electron microscope. We combine basic physical optics theory with the transfer matrix analysis of optical transport in nano-layered polymers to address subtleties in the chromatic response of microlenses made from these materials. In particular this method explains the len's behavior in and around the reflection band of the materials. We wish to acknowledge support of funds from NSF through its Center for Layered Polymeric Systems (CLiPS) at Case Western Reserve University.

Refractive multiple optical tweezers for parallel biochemical analysis in micro-fluidics

NASA Astrophysics Data System (ADS)

Merenda, Fabrice; Rohner, Johann; Pascoal, Pedro; Fournier, Jean-Marc; Vogel, Horst; Salathé, René-Paul

2007-02-01

We present a multiple laser tweezers system based on refractive optics. The system produces an array of 100 optical traps thanks to a refractive microlens array, whose focal plane is imaged into the focal plane of a high-NA microscope objective. This refractive multi-tweezers system is combined to micro-fluidics, aiming at performing simultaneous biochemical reactions on ensembles of free floating objects. Micro-fluidics allows both transporting the particles to the trapping area, and conveying biochemical reagents to the trapped particles. Parallel trapping in micro-fluidics is achieved with polystyrene beads as well as with native vesicles produced from mammalian cells. The traps can hold objects against fluid flows exceeding 100 micrometers per second. Parallel fluorescence excitation and detection on the ensemble of trapped particles is also demonstrated. Additionally, the system is capable of selectively and individually releasing particles from the tweezers array using a complementary steerable laser beam. Strategies for high-yield particle capture and individual particle release in a micro-fluidic environment are discussed. A comparison with diffractive optical tweezers enhances the pros and cons of refractive systems.

VLA telemetry performance with concatenated coding for Voyager at Neptune

NASA Technical Reports Server (NTRS)

Dolinar, S. J., Jr.

1988-01-01

Current plans for supporting the Voyager encounter at Neptune include the arraying of the Deep Space Network (DSN) antennas at Goldstone, California, with the National Radio Astronomy Observatory's Very Large Array (VLA) in New Mexico. Not designed as a communications antenna, the VLA signal transmission facility suffers a disadvantage in that the received signal is subjected to a gap or blackout period of approximately 1.6 msec once every 5/96 sec control cycle. Previous analyses showed that the VLA data gaps could cause disastrous performance degradation in a VLA stand-alone system and modest degradation when the VLA is arrayed equally with Goldstone. New analysis indicates that the earlier predictions for concatenated code performance were overly pessimistic for most combinations of system parameters, including those of Voyager-VLA. The periodicity of the VLA gap cycle tends to guarantee that all Reed-Solomon codewords will receive an average share of erroneous symbols from the gaps. However, large deterministic fluctuations in the number of gapped symbols from codeword to codeword may occur for certain combinations of code parameters, gap cycle parameters, and data rates. Several mechanisms for causing these fluctuations are identified and analyzed. Even though graceful degradation is predicted for the Voyager-VLA parameters, catastrophic degradation greater than 2 dB can occur for a VLA stand-alone system at certain non-Voyager data rates inside the range of the actual Voyager rates. Thus, it is imperative that all of the Voyager-VLA parameters be very accurately known and precisely controlled.

Supercritical fluid chromatography-photodiode array detection-electrospray ionization mass spectrometry as a framework for impurity fate mapping in the development and manufacture of drug substances.

PubMed

Pirrone, Gregory F; Mathew, Rose M; Makarov, Alexey A; Bernardoni, Frank; Klapars, Artis; Hartman, Robert; Limanto, John; Regalado, Erik L

2018-03-30

Impurity fate and purge studies are critical in order to establish an effective impurity control strategy for approval of the commercial filing application of new medicines. Reversed phase liquid chromatography-diode array-mass spectrometry (RPLC-DAD-MS) has traditionally been the preferred tool for impurity fate mapping. However, separation of some reaction mixtures by LC can be very problematic requiring combination LC-UV for area % analysis and a different LC-MS method for peak identification. In addition, some synthetic intermediates might be chemically susceptible to the aqueous conditions used in RPLC separations. In this study, the use of supercritical fluid chromatography-photodiode array-electrospray ionization mass spectrometry (SFC-PDA-ESIMS) for fate and purge of two specified impurities in the 1-uridine starting material from the synthesis of a bis-piv 2'keto-uridine, an intermediate in the synthesis of uprifosbuvir, a treatment under investigation for chronic hepatitis C infection. Readily available SFC instrumentation with a Chiralpak IC column (4.6 × 150 mm, 3 μm) and ethanol: carbon dioxide based mobile phase eluent enabled the separation of closely related components from complex reaction mixtures where RLPC failed to deliver optimal chromatographic performance. These results illustrate how SFC combined with PDA and ESI-MS detection can become a powerful tool for direct impurity fate mapping across multiple reaction steps. Copyright © 2018 Elsevier B.V. All rights reserved.

Coil-to-coil physiological noise correlations and their impact on functional MRI time-series signal-to-noise ratio.

PubMed

Triantafyllou, Christina; Polimeni, Jonathan R; Keil, Boris; Wald, Lawrence L

2016-12-01

Physiological nuisance fluctuations ("physiological noise") are a major contribution to the time-series signal-to-noise ratio (tSNR) of functional imaging. While thermal noise correlations between array coil elements have a well-characterized effect on the image Signal to Noise Ratio (SNR 0 ), the element-to-element covariance matrix of the time-series fluctuations has not yet been analyzed. We examine this effect with a goal of ultimately improving the combination of multichannel array data. We extend the theoretical relationship between tSNR and SNR 0 to include a time-series noise covariance matrix Ψ t , distinct from the thermal noise covariance matrix Ψ 0 , and compare its structure to Ψ 0 and the signal coupling matrix SS H formed from the signal intensity vectors S. Inclusion of the measured time-series noise covariance matrix into the model relating tSNR and SNR 0 improves the fit of experimental multichannel data and is shown to be distinct from Ψ 0 or SS H . Time-series noise covariances in array coils are found to differ from Ψ 0 and more surprisingly, from the signal coupling matrix SS H . Correct characterization of the time-series noise has implications for the analysis of time-series data and for improving the coil element combination process. Magn Reson Med 76:1708-1719, 2016. © 2016 International Society for Magnetic Resonance in Medicine. © 2016 International Society for Magnetic Resonance in Medicine.

Rectenna array measurement results. [Satellite power transmission and reception

NASA Technical Reports Server (NTRS)

Dickinson, R. M.

1980-01-01

The measured performance characteristics of a rectenna array are reviewed and compared to the performance of a single element. It is shown that the performance may be extrapolated from the individual element to that of the collection of elements. Techniques for current and voltage combining are demonstrated. The array performance as a function of various operating parameters is characterized and techniques for overvoltage protection and automatic fault clearing in the array are demonstrated. A method for detecting failed elements also exists. Instrumentation for deriving performance effectiveness is described. Measured harmonic radiation patterns and fundamental frequency scattered patterns for a low level illumination rectenna array are presented.

Phase-locked, high power, mid-infrared quantum cascade laser arrays

NASA Astrophysics Data System (ADS)

Zhou, W.; Slivken, S.; Razeghi, M.

2018-04-01

We demonstrate phase-locked, high power quantum cascade laser arrays, which are combined using a monolithic, tree array multimode interferometer, with emission wavelengths around 4.8 μm. A maximum output power of 15 W was achieved from an eight-element laser array, which has only a slightly higher threshold current density and a similar slope efficiency compared to a Fabry-Perot laser of the same length. Calculated multimode interferometer splitting loss is on the order of 0.27 dB for the in-phase supermode. In-phase supermode operation with nearly ideal behavior is demonstrated over the working current range of the array.

Serial multiplier arrays for parallel computation

NASA Technical Reports Server (NTRS)

Winters, Kel

1990-01-01

Arrays of systolic serial-parallel multiplier elements are proposed as an alternative to conventional SIMD mesh serial adder arrays for applications that are multiplication intensive and require few stored operands. The design and operation of a number of multiplier and array configurations featuring locality of connection, modularity, and regularity of structure are discussed. A design methodology combining top-down and bottom-up techniques is described to facilitate development of custom high-performance CMOS multiplier element arrays as well as rapid synthesis of simulation models and semicustom prototype CMOS components. Finally, a differential version of NORA dynamic circuits requiring a single-phase uncomplemented clock signal introduced for this application.

Engaging spaces: Intimate electro-acoustic display in alternative performance venues

NASA Astrophysics Data System (ADS)

Bahn, Curtis; Moore, Stephan

2004-05-01

In past presentations to the ASA, we have described the design and construction of four generations of unique spherical speakers (multichannel, outward-radiating geodesic speaker arrays) and Sensor-Speaker-Arrays, (SenSAs: combinations of various sensor devices with outward-radiating multichannel speaker arrays). This presentation will detail the ways in which arrays of these speakers have been employed in alternative performance venues-providing presence and intimacy in the performance of electro-acoustic chamber music and sound installation, while engaging natural and unique acoustical qualities of various locations. We will present documentation of the use of multichannel sonic diffusion arrays in small clubs, ``black-box'' theaters, planetariums, and art galleries.

Fish-Eye Observing with Phased Array Radio Telescopes

NASA Astrophysics Data System (ADS)

Wijnholds, S. J.

The radio astronomical community is currently developing and building several new radio telescopes based on phased array technology. These telescopes provide a large field-of-view, that may in principle span a full hemisphere. This makes calibration and imaging very challenging tasks due to the complex source structures and direction dependent radio wave propagation effects. In this thesis, calibration and imaging methods are developed based on least squares estimation of instrument and source parameters. Monte Carlo simulations and actual observations with several prototype show that this model based approach provides statistically and computationally efficient solutions. The error analysis provides a rigorous mathematical framework to assess the imaging performance of current and future radio telescopes in terms of the effective noise, which is the combined effect of propagated calibration errors, noise in the data and source confusion.

Radiometrically accurate scene-based nonuniformity correction for array sensors.

PubMed

Ratliff, Bradley M; Hayat, Majeed M; Tyo, J Scott

2003-10-01

A novel radiometrically accurate scene-based nonuniformity correction (NUC) algorithm is described. The technique combines absolute calibration with a recently reported algebraic scene-based NUC algorithm. The technique is based on the following principle: First, detectors that are along the perimeter of the focal-plane array are absolutely calibrated; then the calibration is transported to the remaining uncalibrated interior detectors through the application of the algebraic scene-based algorithm, which utilizes pairs of image frames exhibiting arbitrary global motion. The key advantage of this technique is that it can obtain radiometric accuracy during NUC without disrupting camera operation. Accurate estimates of the bias nonuniformity can be achieved with relatively few frames, which can be fewer than ten frame pairs. Advantages of this technique are discussed, and a thorough performance analysis is presented with use of simulated and real infrared imagery.

Field Programmable Gate Array Reliability Analysis Guidelines for Launch Vehicle Reliability Block Diagrams

NASA Technical Reports Server (NTRS)

Al Hassan, Mohammad; Britton, Paul; Hatfield, Glen Spencer; Novack, Steven D.

2017-01-01

Field Programmable Gate Arrays (FPGAs) integrated circuits (IC) are one of the key electronic components in today's sophisticated launch and space vehicle complex avionic systems, largely due to their superb reprogrammable and reconfigurable capabilities combined with relatively low non-recurring engineering costs (NRE) and short design cycle. Consequently, FPGAs are prevalent ICs in communication protocols and control signal commands. This paper will identify reliability concerns and high level guidelines to estimate FPGA total failure rates in a launch vehicle application. The paper will discuss hardware, hardware description language, and radiation induced failures. The hardware contribution of the approach accounts for physical failures of the IC. The hardware description language portion will discuss the high level FPGA programming languages and software/code reliability growth. The radiation portion will discuss FPGA susceptibility to space environment radiation.

Tissue matrix arrays for high throughput screening and systems analysis of cell function

PubMed Central

Beachley, Vince Z.; Wolf, Matthew T.; Sadtler, Kaitlyn; Manda, Srikanth S.; Jacobs, Heather; Blatchley, Michael; Bader, Joel S.; Pandey, Akhilesh; Pardoll, Drew; Elisseeff, Jennifer H.

2015-01-01

Cell and protein arrays have demonstrated remarkable utility in the high-throughput evaluation of biological responses; however, they lack the complexity of native tissue and organs. Here, we describe tissue extracellular matrix (ECM) arrays for screening biological outputs and systems analysis. We spotted processed tissue ECM particles as two-dimensional arrays or incorporated them with cells to generate three-dimensional cell-matrix microtissue arrays. We then investigated the response of human stem, cancer, and immune cells to tissue ECM arrays originating from 11 different tissues, and validated the 2D and 3D arrays as representative of the in vivo microenvironment through quantitative analysis of tissue-specific cellular responses, including matrix production, adhesion and proliferation, and morphological changes following culture. The biological outputs correlated with tissue proteomics, and network analysis identified several proteins linked to cell function. Our methodology enables broad screening of ECMs to connect tissue-specific composition with biological activity, providing a new resource for biomaterials research and translation. PMID:26480475

Low-background detector arrays for infrared astronomy

NASA Technical Reports Server (NTRS)

Mccreight, C. R.; Estrada, J. A.; Goebel, J. H.; Mckelvey, M. E.; Mckibbin, D. D.; Mcmurray, R. E., Jr.; Weber, T. T.

1989-01-01

The status of a program which develops and characterizes integrated infrared (IR) detector array technology for space astronomical applications is described. The devices under development include intrinsic, extrinsic silicon, and extrinsic germanium detectors, coupled to silicon readout electronics. Low-background laboratory test results include measurements of responsivity, noise, dark current, temporal response, and the effects of gamma-radiation. In addition, successful astronomical imagery has been obtained on some arrays from this program. These two aspects of the development combine to demonstrate the strong potential for integrated array technology for IR space astronomy.

Synthetic aperture radar images with composite azimuth resolution

DOEpatents

Bielek, Timothy P; Bickel, Douglas L

2015-03-31

A synthetic aperture radar (SAR) image is produced by using all phase histories of a set of phase histories to produce a first pixel array having a first azimuth resolution, and using less than all phase histories of the set to produce a second pixel array having a second azimuth resolution that is coarser than the first azimuth resolution. The first and second pixel arrays are combined to produce a third pixel array defining a desired SAR image that shows distinct shadows of moving objects while preserving detail in stationary background clutter.

Generation of dark hollow beam via coherent combination based on adaptive optics.

PubMed

Zheng, Yi; Wang, Xiaohua; Shen, Feng; Li, Xinyang

2010-12-20

A novel method for generating a dark hollow beam (DHB) is proposed and studied both theoretically and experimentally. A coherent combination technique for laser arrays is implemented based on adaptive optics (AO). A beam arraying structure and an active segmented mirror are designed and described. Piston errors are extracted by a zero-order interference detection system with the help of a custom-made photo-detectors array. An algorithm called the extremum approach is adopted to calculate feedback control signals. A dynamic piston error is imported by LiNbO₃ to test the capability of the AO servo. In a closed loop the stable and clear DHB is obtained. The experimental results confirm the feasibility of the concept.

Micromachined electrode array

DOEpatents

Okandan, Murat; Wessendorf, Kurt O.

2007-12-11

An electrode array is disclosed which has applications for neural stimulation and sensing. The electrode array, in certain embodiments, can include a plurality of electrodes each of which is flexibly attached to a common substrate using a plurality of springs to allow the electrodes to move independently. In other embodiments of the electrode array, the electrodes can be fixed to the substrate. The electrode array can be formed from a combination of bulk and surface micromachining, and can include electrode tips having an electroplated metal (e.g. platinum, iridium, gold or titanium) or a metal oxide (e.g. iridium oxide) for biocompatibility. The electrode array can be used to form a part of a neural prosthesis, and is particularly well adapted for use in an implantable retinal prosthesis.

Near-field investigation of the effect of the array edge on the resonance of loop frequency selective surface elements at mid-infrared wavelengths.

PubMed

Tucker, Eric; D' Archangel, Jeffrey; Raschke, Markus B; Boreman, Glenn

2015-05-04

Mid-infrared scattering scanning near-field optical microscopy, in combination with far-field infrared spectroscopy, and simulations, was employed to investigate the effect of mutual-element coupling towards the edge of arrays of loop elements acting as frequency selective surfaces (FSSs). Two different square loop arrays on ZnS over a ground plane, resonant at 10.3 µm, were investigated. One array had elements that were closely spaced while the other array had elements with greater inter-element spacing. In addition to the dipolar resonance, we observed a new emergent resonance associated with the edge of the closely-spaced array as a finite size effect, due to the broken translational invariance.

Analysis of volcano-related seismicity to constrain the magmatic plumbing system beneath Fogo, Cape Verde, by (multi-)array techniques

NASA Astrophysics Data System (ADS)

Dietrich, Carola; Wölbern, Ingo; Faria, Bruno; Rümpker, Georg

2017-04-01

Fogo is the only island of the Cape Verde archipelago with regular occurring volcanic eruptions since its discovery in the 15th century. The volcanism of the archipelago originates from a mantle plume beneath an almost stationary tectonic plate. With an eruption interval of approximately 20 years, Fogo belongs to the most active oceanic volcanoes. The latest eruption started in November 2014 and ceased in February 2015. This study aims to characterize and investigate the seismic activity and the magmatic plumbing system of Fogo, which is believed to be related to a magmatic source close to the neighboring island of Brava. According to previous studies, using conventional seismic network configurations, most of the seismic activity occurs offshore. Therefore, seismological array techniques represent powerful tools in investigating earthquakes and other volcano-related events located outside of the networks. Another advantage in the use of seismic arrays is their possibility to detect events of relatively small magnitude and to locate seismic signals without a clear onset of phases, such as volcanic tremors. Since October 2015 we have been operating a test array on Fogo as part of a pilot study. This array consists of 10 seismic stations, distributed in a circular shape with an aperture of 700 m. The stations are equipped with Omnirecs CUBE dataloggers, and either 4.5 Hz geophones (7 stations) or Trillium-Compact broad-band seismometers (3 stations). In January 2016 we installed three additional broad-band stations distributed across the island of Fogo to improve the capabilities for event localization. The data of the pilot study is dominated by seismic activity around Brava, but also exhibit tremors and hybrid events of unknown origin within the caldera of Fogo volcano. The preliminary analysis of these events includes the characterization and localization of the different event types using seismic array processing in combination with conventional localization methods. In the beginning of August 2016, a "seismic crisis" occurred on the island of Brava which led to the evacuation of a village. The seismic activity recorded by our instruments on Fogo exhibits more than 40 earthquakes during this time. Locations and magnitudes of these events will be presented. In January 2017 the pilot project discussed here will be complemented by three additional seismic arrays (two on Fogo, one on Brava) to improve seismic event localization and structural imaging based on scattered seismic phases by using multi-array techniques. Initial recordings from the new arrays are expected to be available by April 2017.

Parallel processing in finite element structural analysis

NASA Technical Reports Server (NTRS)

Noor, Ahmed K.

1987-01-01

A brief review is made of the fundamental concepts and basic issues of parallel processing. Discussion focuses on parallel numerical algorithms, performance evaluation of machines and algorithms, and parallelism in finite element computations. A computational strategy is proposed for maximizing the degree of parallelism at different levels of the finite element analysis process including: 1) formulation level (through the use of mixed finite element models); 2) analysis level (through additive decomposition of the different arrays in the governing equations into the contributions to a symmetrized response plus correction terms); 3) numerical algorithm level (through the use of operator splitting techniques and application of iterative processes); and 4) implementation level (through the effective combination of vectorization, multitasking and microtasking, whenever available).

Focal activation of primary visual cortex following supra-choroidal electrical stimulation of the retina: Intrinsic signal imaging and linear model analysis.

PubMed

Cloherty, Shaun L; Hietanen, Markus A; Suaning, Gregg J; Ibbotson, Michael R

2010-01-01

We performed optical intrinsic signal imaging of cat primary visual cortex (Area 17 and 18) while delivering bipolar electrical stimulation to the retina by way of a supra-choroidal electrode array. Using a general linear model (GLM) analysis we identified statistically significant (p < 0.01) activation in a localized region of cortex following supra-threshold electrical stimulation at a single retinal locus. (1) demonstrate that intrinsic signal imaging combined with linear model analysis provides a powerful tool for assessing cortical responses to prosthetic stimulation, and (2) confirm that supra-choroidal electrical stimulation can achieve localized activation of the cortex consistent with focal activation of the retina.

Nucleic acid arrays and methods of synthesis

DOEpatents

Sabanayagam, Chandran R.; Sano, Takeshi; Misasi, John; Hatch, Anson; Cantor, Charles

2001-01-01

The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.

Bottom Interaction in Long Range Acoustic Propagation

DTIC Science & Technology

2006-09-30

Pacific Ocean utilizing controlled sources and vertical and horizontal receiver arrays . Broadband sources are considered with typical center...The LOAPEX (Long-range Ocean Acoustic Propagation Experiment) vertical line arrays (VLA) are described on page 1 of the LOAPEX cruise report: " The...hydrophone arrays on the two combined VLAs covered most of the 5-km water column. We refer to one of the VLAs as the deep VLA (DVLA), located at

Infrared sensors for Earth observation missions

NASA Astrophysics Data System (ADS)

Ashcroft, P.; Thorne, P.; Weller, H.; Baker, I.

2007-10-01

SELEX S&AS is developing a family of infrared sensors for earth observation missions. The spectral bands cover shortwave infrared (SWIR) channels from around 1μm to long-wave infrared (LWIR) channels up to 15μm. Our mercury cadmium telluride (MCT) technology has enabled a sensor array design that can satisfy the requirements of all of the SWIR and medium-wave infrared (MWIR) bands with near-identical arrays. This is made possible by the combination of a set of existing technologies that together enable a high degree of flexibility in the pixel geometry, sensitivity, and photocurrent integration capacity. The solution employs a photodiode array under the control of a readout integrated circuit (ROIC). The ROIC allows flexible geometries and in-pixel redundancy to maximise operability and reliability, by combining the photocurrent from a number of photodiodes into a single pixel. Defective or inoperable diodes (or "sub-pixels") can be deselected with tolerable impact on the overall pixel performance. The arrays will be fabricated using the "loophole" process in MCT grown by liquid-phase epitaxy (LPE). These arrays are inherently robust, offer high quantum efficiencies and have been used in previous space programs. The use of loophole arrays also offers access to SELEX's avalanche photodiode (APD) technology, allowing low-noise, highly uniform gain at the pixel level where photon flux is very low.

Code-modulated interferometric imaging system using phased arrays

NASA Astrophysics Data System (ADS)

Chauhan, Vikas; Greene, Kevin; Floyd, Brian

2016-05-01

Millimeter-wave (mm-wave) imaging provides compelling capabilities for security screening, navigation, and bio- medical applications. Traditional scanned or focal-plane mm-wave imagers are bulky and costly. In contrast, phased-array hardware developed for mass-market wireless communications and automotive radar promise to be extremely low cost. In this work, we present techniques which can allow low-cost phased-array receivers to be reconfigured or re-purposed as interferometric imagers, removing the need for custom hardware and thereby reducing cost. Since traditional phased arrays power combine incoming signals prior to digitization, orthogonal code-modulation is applied to each incoming signal using phase shifters within each front-end and two-bit codes. These code-modulated signals can then be combined and processed coherently through a shared hardware path. Once digitized, visibility functions can be recovered through squaring and code-demultiplexing operations. Pro- vided that codes are selected such that the product of two orthogonal codes is a third unique and orthogonal code, it is possible to demultiplex complex visibility functions directly. As such, the proposed system modulates incoming signals but demodulates desired correlations. In this work, we present the operation of the system, a validation of its operation using behavioral models of a traditional phased array, and a benchmarking of the code-modulated interferometer against traditional interferometer and focal-plane arrays.

A novel lab-on-chip platform with integrated solid phase PCR and Supercritical Angle Fluorescence (SAF) microlens array for highly sensitive and multiplexed pathogen detection.

PubMed

Hung, Tran Quang; Chin, Wai Hoe; Sun, Yi; Wolff, Anders; Bang, Dang Duong

2017-04-15

Solid-phase PCR (SP-PCR) has become increasingly popular for molecular diagnosis and there have been a few attempts to incorporate SP-PCR into lab-on-a-chip (LOC) devices. However, their applicability for on-line diagnosis is hindered by the lack of sensitive and portable on-chip optical detection technology. In this paper, we addressed this challenge by combining the SP-PCR with super critical angle fluorescence (SAF) microlens array embedded in a microchip. We fabricated miniaturized SAF microlens array as part of a microfluidic chamber in thermoplastic material and performed multiplexed SP-PCR directly on top of the SAF microlens array. Attribute to the high fluorescence collection efficiency of the SAF microlens array, the SP-PCR assay on the LOC platform demonstrated a high sensitivity of 1.6 copies/µL, comparable to off-chip detection using conventional laser scanner. The combination of SP-PCR and SAF microlens array allows for on-chip highly sensitive and multiplexed pathogen detection with low-cost and compact optical components. The LOC platform would be widely used as a high-throughput biosensor to analyze food, clinical and environmental samples. Copyright © 2016 Elsevier B.V. All rights reserved.

Optimal simulations of ultrasonic fields produced by large thermal therapy arrays using the angular spectrum approach

PubMed Central

Zeng, Xiaozheng; McGough, Robert J.

2009-01-01

The angular spectrum approach is evaluated for the simulation of focused ultrasound fields produced by large thermal therapy arrays. For an input pressure or normal particle velocity distribution in a plane, the angular spectrum approach rapidly computes the output pressure field in a three dimensional volume. To determine the optimal combination of simulation parameters for angular spectrum calculations, the effect of the size, location, and the numerical accuracy of the input plane on the computed output pressure is evaluated. Simulation results demonstrate that angular spectrum calculations performed with an input pressure plane are more accurate than calculations with an input velocity plane. Results also indicate that when the input pressure plane is slightly larger than the array aperture and is located approximately one wavelength from the array, angular spectrum simulations have very small numerical errors for two dimensional planar arrays. Furthermore, the root mean squared error from angular spectrum simulations asymptotically approaches a nonzero lower limit as the error in the input plane decreases. Overall, the angular spectrum approach is an accurate and robust method for thermal therapy simulations of large ultrasound phased arrays when the input pressure plane is computed with the fast nearfield method and an optimal combination of input parameters. PMID:19425640

Demonstration of KHILS two-color IR projection capability

NASA Astrophysics Data System (ADS)

Jones, Lawrence E.; Coker, Jason S.; Garbo, Dennis L.; Olson, Eric M.; Murrer, Robert Lee, Jr.; Bergin, Thomas P.; Goldsmith, George C., II; Crow, Dennis R.; Guertin, Andrew W.; Dougherty, Michael; Marler, Thomas M.; Timms, Virgil G.

1998-07-01

For more than a decade, there has been considerable discussion about using different IR bands for the detection of low contrast military targets. Theory predicts that a target can have little to no contrast against the background in one IR band while having a discernible signature in another IR band. A significant amount of effort has been invested towards establishing hardware that is capable of simultaneously imaging in two IR bands to take advantage of this phenomenon. Focal plane arrays (FPA) are starting to materialize with this simultaneous two-color imaging capability. The Kinetic Kill Vehicle Hardware-in-the-loop Simulator (KHILS) team of the Air Force Research Laboratory and the Guided Weapons Evaluation Facility (GWEF), both at Eglin AFB, FL, have spent the last 10 years developing the ability to project dynamic IR scenes to imaging IR seekers. Through the Wideband Infrared Scene Projector (WISP) program, the capability to project two simultaneous IR scenes to a dual color seeker has been established at KHILS. WISP utilizes resistor arrays to produce the IR energy. Resistor arrays are not ideal blackbodies. The projection of two IR colors with resistor arrays, therefore, requires two optically coupled arrays. This paper documents the first demonstration of two-color simultaneous projection at KHILS. Agema cameras were used for the measurements. The Agema's HgCdTe detector has responsivity from 4 to 14 microns. A blackbody and two IR filters (MWIR equals 4.2 t 7.4 microns, LWIR equals 7.7 to 13 microns) were used to calibrate the Agema in two bands. Each filter was placed in front of the blackbody one at a time, and the temperature of the blackbody was stepped up in incremental amounts. The output counts from the Agema were recorded at each temperature. This calibration process established the radiance to Agema output count curves for the two bands. The WISP optical system utilizes a dichroic beam combiner to optically couple the two resistor arrays. The transmission path of the beam combiner provided the LWIR (6.75 to 12 microns), while the reflective path produced the MWIR (3 to 6.5 microns). Each resistor array was individually projected into the Agema through the beam combiner at incremental output levels. Once again the Agema's output counts were recorded at each resistor array output level. These projections established the resistor array output to Agema count curves for the MWIR and LWIR resistor arrays. Using the radiance to Agema counts curves, the MWIR and LWIR resistor array output to radiance curves were established. With the calibration curves established, a two-color movie was projected and compared to the generated movie radiance values. By taking care to correctly account for the spectral qualities of the Agema camera, the calibration filters, and the diachroic beam combiner, the projections matched the theoretical calculations. In the near future, a Lockheed- Martin Multiple Quantum Well camera with true two-color IR capability will be tested.

Design and integration of an all-in-one biomicrofluidic chip

PubMed Central

Liu, Liyu; Cao, Wenbin; Wu, Jingbo; Wen, Weijia; Chang, Donald Choy; Sheng, Ping

2008-01-01

We demonstrate a highly integrated microfluidic chip with the function of DNA amplification. The integrated chip combines giant electrorheological-fluid actuated micromixer and micropump with a microheater array, all formed using soft lithography. Internal functional components are based on polydimethylsiloxane (PDMS) and silver∕carbon black-PDMS composites. The system has the advantages of small size with a high degree of integration, high polymerase chain reaction efficiency, digital control and simple fabrication at low cost. This integration approach shows promise for a broad range of applications in chemical synthesis and biological sensing∕analysis, as different components can be combined to target desired functionalities, with flexible designs of different microchips easily realizable through soft lithography. PMID:19693370

Working with and Visualizing Big Data Efficiently with Python for the DARPA XDATA Program

DTIC Science & Technology

2017-08-01

same function to be used with scalar inputs, input arrays of the same shape, or even input arrays of dimensionality in some cases. Most of the math ... math operations on values ● Split-apply-combine: similar to group-by operations in databases ● Join: combine two datasets using common columns 4.3.3...Numba - Continue to increase SIMD performance with support for fast math flags and improved support for AVX, Intel’s large vector

Synthesis of Nanosecond Ultrawideband Radiation Pulses

NASA Astrophysics Data System (ADS)

Koshelev, V. I.; Plisko, V. V.; Sevostyanov, E. A.

2017-12-01

The synthesis of electromagnetic pulses with an extended spectrum by summing pulses of different duration in free space has been studied. The radiation spectrum has been estimated analytically for a 4-element array of combined antennas excited by bipolar voltage pulses of duration 0.5, 1, 2, and 3 ns. It has been shown experimentally that radiation with a spectral width of more than three octaves can be produced using a 2×2 array of combined antennas excited by bipolar pulses of duration 2 and 3 ns.

Smart photodetector arrays for error control in page-oriented optical memory

NASA Astrophysics Data System (ADS)

Schaffer, Maureen Elizabeth

1998-12-01

Page-oriented optical memories (POMs) have been proposed to meet high speed, high capacity storage requirements for input/output intensive computer applications. This technology offers the capability for storage and retrieval of optical data in two-dimensional pages resulting in high throughput data rates. Since currently measured raw bit error rates for these systems fall several orders of magnitude short of industry requirements for binary data storage, powerful error control codes must be adopted. These codes must be designed to take advantage of the two-dimensional memory output. In addition, POMs require an optoelectronic interface to transfer the optical data pages to one or more electronic host systems. Conventional charge coupled device (CCD) arrays can receive optical data in parallel, but the relatively slow serial electronic output of these devices creates a system bottleneck thereby eliminating the POM advantage of high transfer rates. Also, CCD arrays are "unintelligent" interfaces in that they offer little data processing capabilities. The optical data page can be received by two-dimensional arrays of "smart" photo-detector elements that replace conventional CCD arrays. These smart photodetector arrays (SPAs) can perform fast parallel data decoding and error control, thereby providing an efficient optoelectronic interface between the memory and the electronic computer. This approach optimizes the computer memory system by combining the massive parallelism and high speed of optics with the diverse functionality, low cost, and local interconnection efficiency of electronics. In this dissertation we examine the design of smart photodetector arrays for use as the optoelectronic interface for page-oriented optical memory. We review options and technologies for SPA fabrication, develop SPA requirements, and determine SPA scalability constraints with respect to pixel complexity, electrical power dissipation, and optical power limits. Next, we examine data modulation and error correction coding for the purpose of error control in the POM system. These techniques are adapted, where possible, for 2D data and evaluated as to their suitability for a SPA implementation in terms of BER, code rate, decoder time and pixel complexity. Our analysis shows that differential data modulation combined with relatively simple block codes known as array codes provide a powerful means to achieve the desired data transfer rates while reducing error rates to industry requirements. Finally, we demonstrate the first smart photodetector array designed to perform parallel error correction on an entire page of data and satisfy the sustained data rates of page-oriented optical memories. Our implementation integrates a monolithic PN photodiode array and differential input receiver for optoelectronic signal conversion with a cluster error correction code using 0.35-mum CMOS. This approach provides high sensitivity, low electrical power dissipation, and fast parallel correction of 2 x 2-bit cluster errors in an 8 x 8 bit code block to achieve corrected output data rates scalable to 102 Gbps in the current technology increasing to 1.88 Tbps in 0.1-mum CMOS.

Slotline fed microstrip antenna array modules

NASA Technical Reports Server (NTRS)

Lo, Y. T.; Oberhart, M. L.; Brenneman, J. S.; Aoyagi, P.; Moore, J.; Lee, R. Q. H.

1988-01-01

A feed network comprised of a combination of coplanar waveguide and slot transmission line is described for use in an array module of four microstrip elements. Examples of the module incorporating such networks are presented as well as experimentally obtained impedance and radiation characteristics.

Electronic Switch Arrays for Managing Microbattery Arrays

NASA Technical Reports Server (NTRS)

Mojarradi, Mohammad; Alahmad, Mahmoud; Sukumar, Vinesh; Zghoul, Fadi; Buck, Kevin; Hess, Herbert; Li, Harry; Cox, David

2008-01-01

Integrated circuits have been invented for managing the charging and discharging of such advanced miniature energy-storage devices as planar arrays of microscopic energy-storage elements [typically, microscopic electrochemical cells (microbatteries) or microcapacitors]. The architecture of these circuits enables implementation of the following energy-management options: dynamic configuration of the elements of an array into a series or parallel combination of banks (subarrarys), each array comprising a series of parallel combination of elements; direct addressing of individual banks for charging/or discharging; and, disconnection of defective elements and corresponding reconfiguration of the rest of the array to utilize the remaining functional elements to obtain the desited voltage and current performance. An integrated circuit according to the invention consists partly of a planar array of field-effect transistors that function as switches for routing electric power among the energy-storage elements, the power source, and the load. To connect the energy-storage elements to the power source for charging, a specific subset of switches is closed; to connect the energy-storage elements to the load for discharging, a different specific set of switches is closed. Also included in the integrated circuit is circuitry for monitoring and controlling charging and discharging. The control and monitoring circuitry, the switching transistors, and interconnecting metal lines are laid out on the integrated-circuit chip in a pattern that registers with the array of energy-storage elements. There is a design option to either (1) fabricate the energy-storage elements in the corresponding locations on, and as an integral part of, this integrated circuit; or (2) following a flip-chip approach, fabricate the array of energy-storage elements on a separate integrated-circuit chip and then align and bond the two chips together.

A Comparative Study of Automated Infrasound Detectors - PMCC and AFD with Analyst Review.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, Junghyun; Hayward, Chris; Zeiler, Cleat

Automated detections calculated by the progressive multi-channel correlation (PMCC) method (Cansi, 1995) and the adaptive F detector (AFD) (Arrowsmith et al., 2009) are compared to the signals identified by five independent analysts. Each detector was applied to a four-hour time sequence recorded by the Korean infrasound array CHNAR. This array was used because it is composed of both small (<100 m) and large (~1000 m) aperture element spacing. The four hour time sequence contained a number of easily identified signals under noise conditions that have average RMS amplitudes varied from 1.2 to 4.5 mPa (1 to 5 Hz), estimated withmore » running five-minute window. The effectiveness of the detectors was estimated for the small aperture, large aperture, small aperture combined with the large aperture, and full array. The full and combined arrays performed the best for AFD under all noise conditions while the large aperture array had the poorest performance for both detectors. PMCC produced similar results as AFD under the lower noise conditions, but did not produce as dramatic an increase in detections using the full and combined arrays. Both automated detectors and the analysts produced a decrease in detections under the higher noise conditions. Comparing the detection probabilities with Estimated Receiver Operating Characteristic (EROC) curves we found that the smaller value of consistency for PMCC and the larger p-value for AFD had the highest detection probability. These parameters produced greater changes in detection probability than estimates of the false alarm rate. The detection probability was impacted the most by noise level, with low noise (average RMS amplitude of 1.7 mPa) having an average detection probability of ~40% and high noise (average RMS amplitude of 2.9 mPa) average detection probability of ~23%.« less

Large-Scale Interaction Profiling of Protein Domains Through Proteomic Peptide-Phage Display Using Custom Peptidomes.

PubMed

Seo, Moon-Hyeong; Nim, Satra; Jeon, Jouhyun; Kim, Philip M

2017-01-01

Protein-protein interactions are essential to cellular functions and signaling pathways. We recently combined bioinformatics and custom oligonucleotide arrays to construct custom-made peptide-phage libraries for screening peptide-protein interactions, an approach we call proteomic peptide-phage display (ProP-PD). In this chapter, we describe protocols for phage display for the identification of natural peptide binders for a given protein. We finally describe deep sequencing for the analysis of the proteomic peptide-phage display.

Layered Organization in the Coastal Ocean: 4-D Assessment of Thin Layer Structure, Dynamics and Impacts

DTIC Science & Technology

2007-09-30

For example, the differences seen between the waters off of the US Pacific Northwest and the California Bight are almost certainly a reflection of the...the Pacific Northwest were favorable for thin layer development during that study. This is even more evident in those cases where thin layers...approach during the 2005 and 2006 LOCO process study combined time series data from an array of our Ocean Response Coastal Analysis System ( ORCAS ) (Donaghay

Simultaneous data communication and position sensing with an impact ionization engineered avalanche photodiode array for free space optical communication

NASA Astrophysics Data System (ADS)

Ferraro, Mike S.; Mahon, Rita; Rabinovich, William S.; Murphy, James L.; Dexter, James L.; Clark, William R.; Waters, William D.; Vaccaro, Kenneth; Krejca, Brian D.

2017-02-01

Photodetectors in free space optical communication systems perform two functions: reception of data communication signals and position sensing for pointing, tracking, and stabilization. Traditionally, the optical receive path in an FSO system is split into separate paths for data detection and position sensing. The need for separate paths is a consequence of conflicting performance criteria between position sensitive detectors (PSD) and data detectors. Combining the functionality of both detector types requires that the combinational sensor not only have the bandwidth to support high data rate communication but the active area and spatial discrimination to accommodate position sensing. In this paper we present a large area, concentric five element impact ionization engineered avalanche photodiode array rated for bandwidths beyond 1GHz with a measured carrier ionization ratio of less than 0.1 at moderate APD gains. The integration of this array as a combinational sensor in an FSO system is discussed along with the development of a pointing and stabilization algorithm.

Improved intra-array and interarray normalization of peptide microarray phosphorylation for phosphorylome and kinome profiling by rational selection of relevant spots

PubMed Central

Scholma, Jetse; Fuhler, Gwenny M.; Joore, Jos; Hulsman, Marc; Schivo, Stefano; List, Alan F.; Reinders, Marcel J. T.; Peppelenbosch, Maikel P.; Post, Janine N.

2016-01-01

Massive parallel analysis using array technology has become the mainstay for analysis of genomes and transcriptomes. Analogously, the predominance of phosphorylation as a regulator of cellular metabolism has fostered the development of peptide arrays of kinase consensus substrates that allow the charting of cellular phosphorylation events (often called kinome profiling). However, whereas the bioinformatical framework for expression array analysis is well-developed, no advanced analysis tools are yet available for kinome profiling. Especially intra-array and interarray normalization of peptide array phosphorylation remain problematic, due to the absence of “housekeeping” kinases and the obvious fallacy of the assumption that different experimental conditions should exhibit equal amounts of kinase activity. Here we describe the development of analysis tools that reliably quantify phosphorylation of peptide arrays and that allow normalization of the signals obtained. We provide a method for intraslide gradient correction and spot quality control. We describe a novel interarray normalization procedure, named repetitive signal enhancement, RSE, which provides a mathematical approach to limit the false negative results occuring with the use of other normalization procedures. Using in silico and biological experiments we show that employing such protocols yields superior insight into cellular physiology as compared to classical analysis tools for kinome profiling. PMID:27225531

Two-color beam improvement of the colloidal particle lens array assisted surface nanostructuring

NASA Astrophysics Data System (ADS)

Afanasiev, Andrei; Bredikhin, Vladimir; Pikulin, Alexander; Ilyakov, Igor; Shishkin, Boris; Akhmedzhanov, Rinat; Bityurin, Nikita

2015-05-01

We consider laser nanostructuring of the material surface by means of a colloidal particle lens array. Here, the monolayer of dielectric micro- or nanospheres placed on the surface acts as an array of near-field lenses that focus the laser radiation into the multitude of distinct spots, allowing the formation of many structures in a single stage. We show that conversion of a small part of the energy of the femtosecond beam into the second harmonic (SH) is an efficient way to increase the surface density of obtained nanostructures. By combining the fundamental frequency and the SH, one benefits both from the power of the former and from the focusing ability of the latter. This combination provides an efficient nanostructuring with sphere diameter close to the wavelength of the second harmonic. The possibility to create arrays of nanostructures with surface density above 5 × 10 8 cm - 2 with femtosecond Ti:sapphire laser operating at 800 nm was demonstrated by employing 0.45 μm spheres.

Carbon Nanofiber Electrode Array for Neurochemical Monitoring

NASA Technical Reports Server (NTRS)

Koehne, Jessica E.

2017-01-01

A sensor platform based on vertically aligned carbon nanofibers (CNFs) has been developed. Their inherent nanometer scale, high conductivity, wide potential window, good biocompatibility and well-defined surface chemistry make them ideal candidates as biosensor electrodes. Here, we report using vertically aligned CNF as neurotransmitter recording electrodes for application in a smart deep brain stimulation (DBS) device. Our approach combines a multiplexed CNF electrode chip, developed at NASA Ames Research Center, with the Wireless Instantaneous Neurotransmitter Concentration Sensor (WINCS) system, developed at the Mayo Clinic. Preliminary results indicate that the CNF nanoelectrode arrays are easily integrated with WINCS for neurotransmitter detection in a multiplexed array format. In the future, combining CNF based stimulating and recording electrodes with WINCS may lay the foundation for an implantable smart therapeutic system that utilizes neurochemical feedback control while likely resulting in increased DBS application in various neuropsychiatric disorders. In total, our goal is to take advantage of the nanostructure of CNF arrays for biosensing studies requiring ultrahigh sensitivity, high-degree of miniaturization, and selective biofunctionalization.

A waveform diversity method for optimizing 3-d power depositions generated by ultrasound phased arrays.

PubMed

Zeng, Xiaozheng Jenny; Li, Jian; McGough, Robert J

2010-01-01

A waveform-diversity-based approach for 3-D tumor heating is compared to spot scanning for hyperthermia applications. The waveform diversity method determines the excitation signals applied to the phased array elements and produces a beam pattern that closely matches the desired power distribution. The optimization algorithm solves the covariance matrix of the excitation signals through semidefinite programming subject to a series of quadratic cost functions and constraints on the control points. A numerical example simulates a 1444-element spherical-section phased array that delivers heat to a 3-cm-diameter spherical tumor located 12 cm from the array aperture, and the results show that waveform diversity combined with mode scanning increases the heated volume within the tumor while simultaneously decreasing normal tissue heating. Whereas standard single focus and multiple focus methods are often associated with unwanted intervening tissue heating, the waveform diversity method combined with mode scanning shifts energy away from intervening tissues where hotspots otherwise accumulate to improve temperature localization in deep-seated tumors.

Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics.

PubMed

Papadakis, G; Friedt, J M; Eck, M; Rabus, D; Jobst, G; Gizeli, E

2017-09-01

The development of integrated platforms incorporating an acoustic device as the detection element requires addressing simultaneously several challenges of technological and scientific nature. The present work was focused on the design of a microfluidic module, which, combined with a dual or array type Love wave acoustic chip could be applied to biomedical applications and molecular diagnostics. Based on a systematic study we optimized the mechanics of the flow cell attachment and the sealing material so that fluidic interfacing/encapsulation would impose minimal losses to the acoustic wave. We have also investigated combinations of operating frequencies with waveguide materials and thicknesses for maximum sensitivity during the detection of protein and DNA biomarkers. Within our investigations neutravidin was used as a model protein biomarker and unpurified PCR amplified Salmonella DNA as the model genetic target. Our results clearly indicate the need for experimental verification of the optimum engineering and analytical parameters, in order to develop commercially viable systems for integrated analysis. The good reproducibility of the signal together with the ability of the array biochip to detect multiple samples hold promise for the future use of the integrated system in a Lab-on-a-Chip platform for application to molecular diagnostics.

Three junction holographic micro-scale PV system

NASA Astrophysics Data System (ADS)

Wu, Yuechen; Vorndran, Shelby; Ayala Pelaez, Silvana; Kostuk, Raymond K.

2016-09-01

In this work a spectrum splitting micro-scale concentrating PV system is evaluated to increase the conversion efficiency of flat panel PV systems. In this approach, the dispersed spectrum splitting concentration systems is scaled down to a small size and structured in an array. The spectrum splitting configuration allows the use of separate single bandgap PV cells that increase spectral overlap with the incident solar spectrum. This results in an overall increase in the spectral conversion efficiency of the resulting system. In addition other benefits of the micro-scale PV system are retained such reduced PV cell material requirements, more versatile interconnect configurations, and lower heat rejection requirements that can lead to a lower cost system. The system proposed in this work consists of two cascaded off-axis holograms in combination with a micro lens array, and three types of PV cells. An aspherical lens design is made to minimize the dispersion so that higher concentration ratios can be achieved for a three-junction system. An analysis methodology is also developed to determine the optical efficiency of the resulting system, the characteristics of the dispersed spectrum, and the overall system conversion efficiency for a combination of three types of PV cells.

Analysis of polymeric phenolics in red wines using different techniques combined with gel permeation chromatography fractionation.

PubMed

Guadalupe, Zenaida; Soldevilla, Alberto; Sáenz-Navajas, María-Pilar; Ayestarán, Belén

2006-04-21

A multiple-step analytical method was developed to improve the analysis of polymeric phenolics in red wines. With a common initial step based on the fractionation of wine phenolics by gel permeation chromatography (GPC), different analytical techniques were used: high-performance liquid chromatography-diode array detection (HPLC-DAD), HPLC-mass spectrometry (MS), capillary zone electrophoresis (CZE) and spectrophotometry. This method proved to be valid for analyzing different families of phenolic compounds, such as monomeric phenolics and their derivatives, polymeric pigments and proanthocyanidins. The analytical characteristics of fractionation by GPC were studied and the method was fully validated, yielding satisfactory statistical results. GPC fractionation substantially improved the analysis of polymeric pigments by CZE, in terms of response, repeatability and reproducibility. It also represented an improvement in the traditional vanillin assay used for proanthocyanidin (PA) quantification. Astringent proanthocyanidins were also analyzed using a simple combined method that allowed these compounds, for which only general indexes were available, to be quantified.

Comparison of three-way and four-way calibration for the real-time quantitative analysis of drug hydrolysis in complex dynamic samples by excitation-emission matrix fluorescence.

PubMed

Yin, Xiao-Li; Gu, Hui-Wen; Liu, Xiao-Lu; Zhang, Shan-Hui; Wu, Hai-Long

2018-03-05

Multiway calibration in combination with spectroscopic technique is an attractive tool for online or real-time monitoring of target analyte(s) in complex samples. However, how to choose a suitable multiway calibration method for the resolution of spectroscopic-kinetic data is a troubling problem in practical application. In this work, for the first time, three-way and four-way fluorescence-kinetic data arrays were generated during the real-time monitoring of the hydrolysis of irinotecan (CPT-11) in human plasma by excitation-emission matrix fluorescence. Alternating normalization-weighted error (ANWE) and alternating penalty trilinear decomposition (APTLD) were used as three-way calibration for the decomposition of the three-way kinetic data array, whereas alternating weighted residual constraint quadrilinear decomposition (AWRCQLD) and alternating penalty quadrilinear decomposition (APQLD) were applied as four-way calibration to the four-way kinetic data array. The quantitative results of the two kinds of calibration models were fully compared from the perspective of predicted real-time concentrations, spiked recoveries of initial concentration, and analytical figures of merit. The comparison study demonstrated that both three-way and four-way calibration models could achieve real-time quantitative analysis of the hydrolysis of CPT-11 in human plasma under certain conditions. However, it was also found that both of them possess some critical advantages and shortcomings during the process of dynamic analysis. The conclusions obtained in this paper can provide some helpful guidance for the reasonable selection of multiway calibration models to achieve the real-time quantitative analysis of target analyte(s) in complex dynamic systems. Copyright © 2017 Elsevier B.V. All rights reserved.

Solid state lighting component

DOEpatents

Yuan, Thomas; Keller, Bernd; Tarsa, Eric; Ibbetson, James; Morgan, Frederick; Dowling, Kevin; Lys, Ihor

2017-10-17

An LED component according to the present invention comprising an array of LED chips mounted on a submount with the LED chips capable of emitting light in response to an electrical signal. The array can comprise LED chips emitting at two colors of light wherein the LED component emits light comprising the combination of the two colors of light. A single lens is included over the array of LED chips. The LED chip array can emit light of greater than 800 lumens with a drive current of less than 150 milli-Amps. The LED chip component can also operate at temperatures less than 3000 degrees K. In one embodiment, the LED array is in a substantially circular pattern on the submount.

Predictive Array Design. A method for sampling combinatorial chemistry library space.

PubMed

Lipkin, M J; Rose, V S; Wood, J

2002-01-01

A method, Predictive Array Design, is presented for sampling combinatorial chemistry space and selecting a subarray for synthesis based on the experimental design method of Latin Squares. The method is appropriate for libraries with three sites of variation. Libraries with four sites of variation can be designed using the Graeco-Latin Square. Simulated annealing is used to optimise the physicochemical property profile of the sub-array. The sub-array can be used to make predictions of the activity of compounds in the all combinations array if we assume each monomer has a relatively constant contribution to activity and that the activity of a compound is composed of the sum of the activities of its constitutive monomers.

Optical Design of Segmented Hexagon Array Solar Mirror

NASA Technical Reports Server (NTRS)

Huegele, Vince

2000-01-01

A segmented array of mirrors was designed for a solar concentrator test stand at MSFC for firing solar thermal propulsion engines. The 144 mirrors each have a spherical surface to approximate a parabolic concentrator when combined into the entire 18-foot diameter array. The mirror segments are aluminum hexagons that had the surface diamond turned and quartz coated. The array focuses sunlight reflected from a heliostat to a 4 inch diameter spot containing 10 kw of power at the 15-foot focal point. The derivation of the surface figure for the respective mirror elements is shown. The alignment process of the array is discussed and test results of the system's performance is given.

Simultaneous Determination of Eight Hypotensive Drugs of Various Chemical Groups in Pharmaceutical Preparations by HPLC-DAD.

PubMed

Stolarczyk, Mariusz; Hubicka, Urszula; Żuromska-Witek, Barbara; Krzek, Jan

2015-01-01

A new sensitive, simple, rapid, and precise HPLC method with diode array detection has been developed for separation and simultaneous determination of hydrochlorothiazide, furosemide, torasemide, losartane, quinapril, valsartan, spironolactone, and canrenone in combined pharmaceutical dosage forms. The chromatographic analysis of the tested drugs was performed on an ACE C18, 100 Å, 250×4.6 mm, 5 μm particle size column with 0.0.05 M phosphate buffer (pH=3.00)-acetonitrile-methanol (30+20+50 v/v/v) mobile phase at a flow rate of 1.0 mL/min. The column was thermostatted at 25°C. UV detection was performed at 230 nm. Analysis time was 10 min. The elaborated method meets the acceptance criteria for specificity, linearity, sensitivity, accuracy, and precision. The proposed method was successfully applied for the determination of the studied drugs in the selected combined dosage forms.

A new approach in space-time analysis of multivariate hydrological data: Application to Brazil's Nordeste region rainfall

NASA Astrophysics Data System (ADS)

Sicard, Emeline; Sabatier, Robert; Niel, HéLèNe; Cadier, Eric

2002-12-01

The objective of this paper is to implement an original method for spatial and multivariate data, combining a method of three-way array analysis (STATIS) with geostatistical tools. The variables of interest are the monthly amounts of rainfall in the Nordeste region of Brazil, recorded from 1937 to 1975. The principle of the technique is the calculation of a linear combination of the initial variables, containing a large part of the initial variability and taking into account the spatial dependencies. It is a promising method that is able to analyze triple variability: spatial, seasonal, and interannual. In our case, the first component obtained discriminates a group of rain gauges, corresponding approximately to the Agreste, from all the others. The monthly variables of July and August strongly influence this separation. Furthermore, an annual study brings out the stability of the spatial structure of components calculated for each year.

Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2.

PubMed

Wei, Lei; Wang, Qing; Ning, Xuanxuan; Mu, Changkao; Wang, Chunlin; Cao, Ruiwen; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin

2015-03-01

Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. Copyright © 2014 Elsevier Inc. All rights reserved.

APRON: A Cellular Processor Array Simulation and Hardware Design Tool

NASA Astrophysics Data System (ADS)

Barr, David R. W.; Dudek, Piotr

2009-12-01

We present a software environment for the efficient simulation of cellular processor arrays (CPAs). This software (APRON) is used to explore algorithms that are designed for massively parallel fine-grained processor arrays, topographic multilayer neural networks, vision chips with SIMD processor arrays, and related architectures. The software uses a highly optimised core combined with a flexible compiler to provide the user with tools for the design of new processor array hardware architectures and the emulation of existing devices. We present performance benchmarks for the software processor array implemented on standard commodity microprocessors. APRON can be configured to use additional processing hardware if necessary and can be used as a complete graphical user interface and development environment for new or existing CPA systems, allowing more users to develop algorithms for CPA systems.

Hierarchically Structured Co3O4@Pt@MnO2 Nanowire Arrays for High-Performance Supercapacitors

PubMed Central

Xia, Hui; Zhu, Dongdong; Luo, Zhentao; Yu, Yue; Shi, Xiaoqin; Yuan, Guoliang; Xie, Jianping

2013-01-01

Here we proposed a novel architectural design of a ternary MnO2-based electrode – a hierarchical Co3O4@Pt@MnO2 core-shell-shell structure, where the complemental features of the three key components (a well-defined Co3O4 nanowire array on the conductive Ti substrate, an ultrathin layer of small Pt nanoparticles, and a thin layer of MnO2 nanoflakes) are strategically combined into a single entity to synergize and construct a high-performance electrode for supercapacitors. Owing to the high conductivity of the well-defined Co3O4 nanowire arrays, in which the conductivity was further enhanced by a thin metal (Pt) coating layer, in combination with the large surface area provided by the small MnO2 nanoflakes, the as-fabricated Co3O4@Pt@MnO2 nanowire arrays have exhibited high specific capacitances, good rate capability, and excellent cycling stability. The architectural design demonstrated in this study provides a new approach to fabricate high-performance MnO2–based nanowire arrays for constructing next-generation supercapacitors. PMID:24132040

System performance predictions for Space Station Freedom's electric power system

NASA Technical Reports Server (NTRS)

Kerslake, Thomas W.; Hojnicki, Jeffrey S.; Green, Robert D.; Follo, Jeffrey C.

1993-01-01

Space Station Freedom Electric Power System (EPS) capability to effectively deliver power to housekeeping and user loads continues to strongly influence Freedom's design and planned approaches for assembly and operations. The EPS design consists of silicon photovoltaic (PV) arrays, nickel-hydrogen batteries, and direct current power management and distribution hardware and cabling. To properly characterize the inherent EPS design capability, detailed system performance analyses must be performed for early stages as well as for the fully assembled station up to 15 years after beginning of life. Such analyses were repeatedly performed using the FORTRAN code SPACE (Station Power Analysis for Capability Evaluation) developed at the NASA Lewis Research Center over a 10-year period. SPACE combines orbital mechanics routines, station orientation/pointing routines, PV array and battery performance models, and a distribution system load-flow analysis to predict EPS performance. Time-dependent, performance degradation, low earth orbit environmental interactions, and EPS architecture build-up are incorporated in SPACE. Results from two typical SPACE analytical cases are presented: (1) an electric load driven case and (2) a maximum EPS capability case.

Evaluation of stereo-array isotope labeling (SAIL) patterns for automated structural analysis of proteins with CYANA.

PubMed

Ikeya, Teppei; Terauchi, Tsutomu; Güntert, Peter; Kainosho, Masatsune

2006-07-01

Recently we have developed the stereo-array isotope labeling (SAIL) technique to overcome the conventional molecular size limitation in NMR protein structure determination by employing complete stereo- and regiospecific patterns of stable isotopes. SAIL sharpens signals and simplifies spectra without the loss of requisite structural information, thus making large classes of proteins newly accessible to detailed solution structure determination. The automated structure calculation program CYANA can efficiently analyze SAIL-NOESY spectra and calculate structures without manual analysis. Nevertheless, the original SAIL method might not be capable of determining the structures of proteins larger than 50 kDa or membrane proteins, for which the spectra are characterized by many broadened and overlapped peaks. Here we have carried out simulations of new SAIL patterns optimized for minimal relaxation and overlap, to evaluate the combined use of SAIL and CYANA for solving the structures of larger proteins and membrane proteins. The modified approach reduces the number of peaks to nearly half of that observed with uniform labeling, while still yielding well-defined structures and is expected to enable NMR structure determinations of these challenging systems.

Development of MATLAB software to control data acquisition from a multichannel systems multi-electrode array.

PubMed

Messier, Erik

2016-08-01

A Multichannel Systems (MCS) microelectrode array data acquisition (DAQ) unit is used to collect multichannel electrograms (EGM) from a Langendorff perfused rabbit heart system to study sudden cardiac death (SCD). MCS provides software through which data being processed by the DAQ unit can be displayed and saved, but this software's combined utility with MATLAB is not very effective. MCSs software stores recorded EGM data in a MathCad (MCD) format, which is then converted to a text file format. These text files are very large, and it is therefore very time consuming to import the EGM data into MATLAB for real-time analysis. Therefore, customized MATLAB software was developed to control the acquisition of data from the MCS DAQ unit, and provide specific laboratory accommodations for this study of SCD. The developed DAQ unit control software will be able to accurately: provide real time display of EGM signals; record and save EGM signals in MATLAB in a desired format; and produce real time analysis of the EGM signals; all through an intuitive GUI.

On the structure and phase transitions of power-law Poissonian ensembles

NASA Astrophysics Data System (ADS)

Eliazar, Iddo; Oshanin, Gleb

2012-10-01

Power-law Poissonian ensembles are Poisson processes that are defined on the positive half-line, and that are governed by power-law intensities. Power-law Poissonian ensembles are stochastic objects of fundamental significance; they uniquely display an array of fractal features and they uniquely generate a span of important applications. In this paper we apply three different methods—oligarchic analysis, Lorenzian analysis and heterogeneity analysis—to explore power-law Poissonian ensembles. The amalgamation of these analyses, combined with the topology of power-law Poissonian ensembles, establishes a detailed and multi-faceted picture of the statistical structure and the statistical phase transitions of these elemental ensembles.

Label-free integrative pharmacology on-target of drugs at the β2-adrenergic receptor

NASA Astrophysics Data System (ADS)

Ferrie, Ann M.; Sun, Haiyan; Fang, Ye

2011-07-01

We describe a label-free integrative pharmacology on-target (iPOT) method to assess the pharmacology of drugs at the β2-adrenergic receptor. This method combines dynamic mass redistribution (DMR) assays using an array of probe molecule-hijacked cells with similarity analysis. The whole cell DMR assays track cell system-based, ligand-directed, and kinetics-dependent biased activities of the drugs, and translates their on-target pharmacology into numerical descriptors which are subject to similarity analysis. We demonstrate that the approach establishes an effective link between the label-free pharmacology and in vivo therapeutic indications of drugs.

Chemoprevention of Head and Neck Cancer by Simultaneous Blocking of Epidermal Growth Factor Receptor and Cyclooxygenase-2 Signaling Pathways: Preclinical and Clinical Studies

PubMed Central

Shin, Dong M.; Zhang, Hongzheng; Saba, Nabil; Chen, Amy; Nannapaneni, Sreenivas; Amin, A.R.M. Ruhul; Müller, Susan; Lewis, Melinda; Sica, Gabriel; Kono, Scott; Brandes, Johann C.; Grist, William; Moreno-Williams, Rachel; Beitler, Jonathan J.; Thomas, Sufi M.; Chen, Zhengjia; Shin, Hyung Ju C.; Grandis, Jennifer R.; Khuri, Fadlo R.; Chen, Zhuo Georgia

2013-01-01

Purpose We investigated the efficacy and underlying molecular mechanism of a novel chemopreventive strategy combining epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) with cyclooxygenase-2 inhibitor (COX-2I). Experimental Design We examined the inhibition of tumor cell growth by combined EGFR-TKI (erlotinib) and COX-2I (celecoxib) treatment using head and neck cancer (HNC) cell lines and a preventive xenograft model. We studied the antiangiogenic activity of these agents and examined the affected signaling pathways by immunoblotting analysis in tumor cell lysates and immunohistochemistry (IHC) and enzyme immunoassay (EIA) analyses on the mouse xenograft tissues and blood, respectively. Biomarkers in these signaling pathways were studied by IHC, EIA, and an antibody array analysis in samples collected from participants in a phase I chemoprevention trial of erlotinib and celecoxib. Results The combined treatment inhibited HNC cell growth significantly more potently than either single agent alone in cell line and xenograft models, and resulted in greater inhibition of cell cycle progression at G1 phase than either single drug. The combined treatment modulated the EGFR and mTOR signaling pathways. A phase I chemoprevention trial of combined erlotinib and celecoxib revealed an overall pathologic response rate of 71% at time of data analysis. Analysis of tissue samples from participants consistently showed downregulation of EGFR, pERK and pS6 levels after treatment, which correlated with clinical response. Conclusion Treatment with erlotinib combined with celecoxib offers an effective chemopreventive approach through inhibition of EGFR and mTOR pathways, which may serve as potential biomarkers to monitor the intervention of this combination in the clinic. PMID:23422093

Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction)

PubMed Central

Busti, Elena; Bordoni, Roberta; Castiglioni, Bianca; Monciardini, Paolo; Sosio, Margherita; Donadio, Stefano; Consolandi, Clarissa; Rossi Bernardi, Luigi; Battaglia, Cristina; De Bellis, Gianluca

2002-01-01

Background PCR amplification of bacterial 16S rRNA genes provides the most comprehensive and flexible means of sampling bacterial communities. Sequence analysis of these cloned fragments can provide a qualitative and quantitative insight of the microbial population under scrutiny although this approach is not suited to large-scale screenings. Other methods, such as denaturing gradient gel electrophoresis, heteroduplex or terminal restriction fragment analysis are rapid and therefore amenable to field-scale experiments. A very recent addition to these analytical tools is represented by microarray technology. Results Here we present our results using a Universal DNA Microarray approach as an analytical tool for bacterial discrimination. The proposed procedure is based on the properties of the DNA ligation reaction and requires the design of two probes specific for each target sequence. One oligo carries a fluorescent label and the other a unique sequence (cZipCode or complementary ZipCode) which identifies a ligation product. Ligated fragments, obtained in presence of a proper template (a PCR amplified fragment of the 16s rRNA gene) contain either the fluorescent label or the unique sequence and therefore are addressed to the location on the microarray where the ZipCode sequence has been spotted. Such an array is therefore "Universal" being unrelated to a specific molecular analysis. Here we present the design of probes specific for some groups of bacteria and their application to bacterial diagnostics. Conclusions The combined use of selective probes, ligation reaction and the Universal Array approach yielded an analytical procedure with a good power of discrimination among bacteria. PMID:12243651

Digital Mammography with a Mosaic of CCD Arrays

NASA Technical Reports Server (NTRS)

Jalink, Antony, Jr. (Inventor); McAdoo, James A. (Inventor)

1998-01-01

A digital mammography device uses a mosaic of electronic digital imaging arrays to scan an x-ray image is discussed. The mosaic of arrays is repositioned several times to expose different portions of the image, until the entire image is scanned. The data generated by the arrays during each exposure is stored in a computer. After the final exposure, the computer combines data of the several partial images to produce a composite of the original x-ray image. An aperture plate is used to reduce scatter and the overall exposure of the patient to x-rays.

Phased laser array with tailored spectral and coherence properties

DOEpatents

Messerly, Michael J [Danville, CA; Dawson, Jay W [Livermore, CA; Beach, Raymond J [Livermore, CA

2011-03-29

Architectures for coherently combining an array of fiber-based lasers are provided. By matching their lengths to within a few integer multiples of a wavelength, the spatial and temporal properties of a single large laser are replicated, while extending the average or peak pulsed power limit.

Phased laser array with tailored spectral and coherence properties

DOEpatents

Messerly, Michael J; Dawson, Jay W; Beach, Raymond J

2014-05-20

Architectures for coherently combining an array of fiber-based lasers are provided. By matching their lengths to within a few integer multiples of a wavelength, the spatial and temporal properties of a single large laser are replicated, while extending the average or peak pulsed power limit.

Field-Deployable Acoustic Digital Systems for Noise Measurement

NASA Technical Reports Server (NTRS)

Shams, Qamar A.; Wright, Kenneth D.; Lunsford, Charles B.; Smith, Charlie D.

2000-01-01

Langley Research Center (LaRC) has for years been a leader in field acoustic array measurement technique. Two field-deployable digital measurement systems have been developed to support acoustic research programs at LaRC. For several years, LaRC has used the Digital Acoustic Measurement System (DAMS) for measuring the acoustic noise levels from rotorcraft and tiltrotor aircraft. Recently, a second system called Remote Acquisition and Storage System (RASS) was developed and deployed for the first time in the field along with DAMS system for the Community Noise Flight Test using the NASA LaRC-757 aircraft during April, 2000. The test was performed at Airborne Airport in Wilmington, OH to validate predicted noise reduction benefits from alternative operational procedures. The test matrix was composed of various combinations of altitude, cutback power, and aircraft weight. The DAMS digitizes the acoustic inputs at the microphone site and can be located up to 2000 feet from the van which houses the acquisition, storage and analysis equipment. Digitized data from up to 10 microphones is recorded on a Jaz disk and is analyzed post-test by microcomputer system. The RASS digitizes and stores acoustic inputs at the microphone site that can be located up to three miles from the base station and can compose a 3 mile by 3 mile array of microphones. 16-bit digitized data from the microphones is stored on removable Jaz disk and is transferred through a high speed array to a very large high speed permanent storage device. Up to 30 microphones can be utilized in the array. System control and monitoring is accomplished via Radio Frequency (RF) link. This paper will present a detailed description of both systems, along with acoustic data analysis from both systems.

Lectin array and glycogene expression analyses of ovarian cancer cell line A2780 and its cisplatin-resistant derivate cell line A2780-cp.

PubMed

Zhao, Ran; Qin, Wenjun; Qin, Ruihuan; Han, Jing; Li, Can; Wang, Yisheng; Xu, Congjian

2017-01-01

Ovarian cancer is one of the most lethal gynecological malignancies, in which platinum resistance is a common cause of its relapse and death. Glycosylation has been reported to be involved in drug resistance, and glycomic analyses of ovarian cancer may improve our understanding of the mechanisms underlying cancer cell drug resistance and provide potential biomarkers and therapeutic targets. The serous ovarian cancer cell line A2780 and its platinum-resistant counterpart A2780-cp were used in this study. We performed a lectin array analysis to compare the glycosylation patterns of the two cell lines, a gene expression array was employed to probe the differences in glycogenes. Furthermore, the results were verified by lectin blots. A2780-cp cell exhibited stronger intensities of Lens culinaris (LCA) Canavalia ensiformis (ConA), and Lycopersicon esculentum (LEL) and weaker intensities of Sambucus nigra (SNA) lectins. The gene expression array analysis revealed increased expression of Fut8, B3gnt4, B3gnt5, B4galt2 and decreased expression of Fut1 and ST6GalNAc 6 expression were evident in the A2780-cp cells. The lectin blot confirmed the differences in LCA, ConA, SNA and LEL between the A2780 and A2780-cp cells. The combination of the lectin and gene expression analyses showed that the levels of core fucosylation and poly-LacNAc were increased in the A2780-cp cells and the levels of Fuc α1-2(gal β1-4) GlcNAc and α2-6-linked sialic structures were decreased in the A2780-cp cells. These glycans represent potential biomarkers and might be involved in the mechanism of drug resistance in ovarian cancer.

Identifying allelic loss and homozygous deletions in pancreatic cancer without matched normals using high-density single-nucleotide polymorphism arrays.

PubMed

Calhoun, Eric S; Hucl, Tomas; Gallmeier, Eike; West, Kristen M; Arking, Dan E; Maitra, Anirban; Iacobuzio-Donahue, Christine A; Chakravarti, Aravinda; Hruban, Ralph H; Kern, Scott E

2006-08-15

Recent advances in oligonucleotide arrays and whole-genome complexity reduction data analysis now permit the evaluation of tens of thousands of single-nucleotide polymorphisms simultaneously for a genome-wide analysis of allelic status. Using these arrays, we created high-resolution allelotype maps of 26 pancreatic cancer cell lines. The areas of heterozygosity implicitly served to reveal regions of allelic loss. The array-derived maps were verified by a panel of 317 microsatellite markers used in a subset of seven samples, showing a 97.1% concordance between heterozygous calls. Three matched tumor/normal pairs were used to estimate the false-negative and potential false-positive rates for identifying loss of heterozygosity: 3.6 regions (average minimal region of loss, 720,228 bp) and 2.3 regions (average heterozygous gap distance, 4,434,994 bp) per genome, respectively. Genomic fractional allelic loss calculations showed that cumulative levels of allelic loss ranged widely from 17.1% to 79.9% of the haploid genome length. Regional increases in "NoCall" frequencies combined with copy number loss estimates were used to identify 41 homozygous deletions (19 first reports), implicating an additional 13 regions disrupted in pancreatic cancer. Unexpectedly, 23 of these occurred in just two lines (BxPc3 and MiaPaCa2), suggesting the existence of at least two subclasses of chromosomal instability (CIN) patterns, distinguished here by allelic loss and copy number changes (original CIN) and those also highly enriched in the genomic "holes" of homozygous deletions (holey CIN). This study provides previously unavailable high-resolution allelotype and deletion breakpoint maps in widely shared pancreatic cancer cell lines and effectively eliminates the need for matched normal tissue to define informative loci.

The accuracy of estimates of the overturning circulation from basin-wide mooring arrays

NASA Astrophysics Data System (ADS)

Sinha, B.; Smeed, D. A.; McCarthy, G.; Moat, B. I.; Josey, S. A.; Hirschi, J. J.-M.; Frajka-Williams, E.; Blaker, A. T.; Rayner, D.; Madec, G.

2018-01-01

Previous modeling and observational studies have established that it is possible to accurately monitor the Atlantic Meridional Overturning Circulation (AMOC) at 26.5°N using a coast-to-coast array of instrumented moorings supplemented by direct transport measurements in key boundary regions (the RAPID/MOCHA/WBTS Array). The main sources of observational and structural errors have been identified in a variety of individual studies. Here a unified framework for identifying and quantifying structural errors associated with the RAPID array-based AMOC estimates is established using a high-resolution (eddy resolving at low-mid latitudes, eddy permitting elsewhere) ocean general circulation model, which simulates the ocean state between 1978 and 2010. We define a virtual RAPID array in the model in close analogy to the real RAPID array and compare the AMOC estimate from the virtual array with the true model AMOC. The model analysis suggests that the RAPID method underestimates the mean AMOC by ∼1.5 Sv (1 Sv = 106 m3 s-1) at ∼900 m depth, however it captures the variability to high accuracy. We examine three major contributions to the streamfunction bias: (i) due to the assumption of a single fixed reference level for calculation of geostrophic transports, (ii) due to regions not sampled by the array and (iii) due to ageostrophic transport. A key element in (i) and (iii) is use of the model sea surface height to establish the true (or absolute) geostrophic transport. In the upper 2000 m, we find that the reference level bias is strongest and most variable in time, whereas the bias due to unsampled regions is largest below 3000 m. The ageostrophic transport is significant in the upper 1000 m but shows very little variability. The results establish, for the first time, the uncertainty of the AMOC estimate due to the combined structural errors in the measurement design and suggest ways in which the error could be reduced. Our work has applications to basin-wide circulation measurement arrays at other latitudes and in other basins as well as quantifying systematic errors in ocean model estimates of the AMOC at 26.5°N.

The Contribution of Genetic Recombination to CRISPR Array Evolution.

PubMed

Kupczok, Anne; Landan, Giddy; Dagan, Tal

2015-06-16

CRISPR (clustered regularly interspaced short palindromic repeats) is a microbial immune system against foreign DNA. Recognition sequences (spacers) encoded within the CRISPR array mediate the immune reaction in a sequence-specific manner. The known mechanisms for the evolution of CRISPR arrays include spacer acquisition from foreign DNA elements at the time of invasion and array erosion through spacer deletion. Here, we consider the contribution of genetic recombination between homologous CRISPR arrays to the evolution of spacer repertoire. Acquisition of spacers from exogenic arrays via recombination may confer the recipient with immunity against unencountered antagonists. For this purpose, we develop a novel method for the detection of recombination in CRISPR arrays by modeling the spacer order in arrays from multiple strains from the same species. Because the evolutionary signal of spacer recombination may be similar to that of pervasive spacer deletions or independent spacer acquisition, our method entails a robustness analysis of the recombination inference by a statistical comparison to resampled and perturbed data sets. We analyze CRISPR data sets from four bacterial species: two Gammaproteobacteria species harboring CRISPR type I and two Streptococcus species harboring CRISPR type II loci. We find that CRISPR array evolution in Escherichia coli and Streptococcus agalactiae can be explained solely by vertical inheritance and differential spacer deletion. In Pseudomonas aeruginosa, we find an excess of single spacers potentially incorporated into the CRISPR locus during independent acquisition events. In Streptococcus thermophilus, evidence for spacer acquisition by recombination is present in 5 out of 70 strains. Genetic recombination has been proposed to accelerate adaptation by combining beneficial mutations that arose in independent lineages. However, for most species under study, we find that CRISPR evolution is shaped mainly by spacer acquisition and loss rather than recombination. Since the evolution of spacer content is characterized by a rapid turnover, it is likely that recombination is not beneficial for improving phage resistance in the strains under study, or that it cannot be detected in the resolution of intraspecies comparisons. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

Cochlear's unique electrode portfolio now and in the future.

PubMed

von Wallenberg, E; Briggs, R

2014-05-01

To review Cochlear's electrode portfolio and discuss the merits of current and future straight and perimodiolar electrode arrays. To present an update on implant reliability. Performance and hearing preservation data from studies involving the Slim Straight (CI422), Hybrid L24 and Contour Advance electrode array were reviewed. While several studies in past found little difference in performance outcomes between subjects implanted with perimodiolar and straight arrays, recent studies demonstrated that proximity to the modiolus is correlated with better performance. Hearing threshold increase was lowest with the Hybrid L24, closely followed by the slim straight array and was largest with the Contour Advance array. The CI24RE receiver-stimulator used for the three arrays had a cumulative survival of 99% at eight years post implantation. Combining the hearing preservation benefits of slim straight arrays with perimodiolar proximity is the design objective of Cochlear's next generation electrodes.

Phased array feed design technology for Large Aperture Microwave Radiometer (LAMR) Earth observations

NASA Technical Reports Server (NTRS)

Schuman, H. K.

1992-01-01

An assessment of the potential and limitations of phased array antennas in space-based geophysical precision radiometry is described. Mathematical models exhibiting the dependence of system and scene temperatures and system sensitivity on phased array antenna parameters and components such as phase shifters and low noise amplifiers (LNA) are developed. Emphasis is given to minimum noise temperature designs wherein the LNA's are located at the array level, one per element or subarray. Two types of combiners are considered: array lenses (space feeds) and corporate networks. The result of a survey of suitable components and devices is described. The data obtained from that survey are used in conjunction with the mathematical models to yield an assessment of effective array antenna noise temperature for representative geostationary and low Earth orbit systems. Practical methods of calibrating a space-based, phased array radiometer are briefly addressed as well.

Space and power efficient hybrid counters array

DOEpatents

Gara, Alan G [Mount Kisco, NY; Salapura, Valentina [Chappaqua, NY

2009-05-12

A hybrid counter array device for counting events. The hybrid counter array includes a first counter portion comprising N counter devices, each counter device for receiving signals representing occurrences of events from an event source and providing a first count value corresponding to a lower order bits of the hybrid counter array. The hybrid counter array includes a second counter portion comprising a memory array device having N addressable memory locations in correspondence with the N counter devices, each addressable memory location for storing a second count value representing higher order bits of the hybrid counter array. A control device monitors each of the N counter devices of the first counter portion and initiates updating a value of a corresponding second count value stored at the corresponding addressable memory location in the second counter portion. Thus, a combination of the first and second count values provide an instantaneous measure of number of events received.

Space and power efficient hybrid counters array

DOEpatents

Gara, Alan G.; Salapura, Valentina

2010-03-30

A hybrid counter array device for counting events. The hybrid counter array includes a first counter portion comprising N counter devices, each counter device for receiving signals representing occurrences of events from an event source and providing a first count value corresponding to a lower order bits of the hybrid counter array. The hybrid counter array includes a second counter portion comprising a memory array device having N addressable memory locations in correspondence with the N counter devices, each addressable memory location for storing a second count value representing higher order bits of the hybrid counter array. A control device monitors each of the N counter devices of the first counter portion and initiates updating a value of a corresponding second count value stored at the corresponding addressable memory location in the second counter portion. Thus, a combination of the first and second count values provide an instantaneous measure of number of events received.

Quasi-optical antenna-mixer-array design for terahertz frequencies

NASA Technical Reports Server (NTRS)

Guo, Yong; Potter, Kent A.; Rutledge, David B.

1992-01-01

A new quasi-optical antenna-mixer-array design for terahertz frequencies is presented. In the design, antenna and mixer are combined into an entity, based on the technology in which millimeter-wave horn antenna arrays have been fabricated in silicon wafers. It consists of a set of forward- and backward-looking horns made with a set of silicon wafers. The front side is used to receive incoming signal, and the back side is used to feed local oscillator signal. Intermediate frequency is led out from the side of the array. Signal received by the horn array is picked up by antenna probes suspended on thin silicon-oxynitride membranes inside the horns. Mixer diodes will be located on the membranes inside the horns. Modeling of such an antenna-mixer-array design is done on a scaled model at microwave frequencies. The impedance matching, RF and LO isolation, and patterns of the array have been tested and analyzed.

A hyperspectral image projector for hyperspectral imagers

NASA Astrophysics Data System (ADS)

Rice, Joseph P.; Brown, Steven W.; Neira, Jorge E.; Bousquet, Robert R.

2007-04-01

We have developed and demonstrated a Hyperspectral Image Projector (HIP) intended for system-level validation testing of hyperspectral imagers, including the instrument and any associated spectral unmixing algorithms. HIP, based on the same digital micromirror arrays used in commercial digital light processing (DLP*) displays, is capable of projecting any combination of many different arbitrarily programmable basis spectra into each image pixel at up to video frame rates. We use a scheme whereby one micromirror array is used to produce light having the spectra of endmembers (i.e. vegetation, water, minerals, etc.), and a second micromirror array, optically in series with the first, projects any combination of these arbitrarily-programmable spectra into the pixels of a 1024 x 768 element spatial image, thereby producing temporally-integrated images having spectrally mixed pixels. HIP goes beyond conventional DLP projectors in that each spatial pixel can have an arbitrary spectrum, not just arbitrary color. As such, the resulting spectral and spatial content of the projected image can simulate realistic scenes that a hyperspectral imager will measure during its use. Also, the spectral radiance of the projected scenes can be measured with a calibrated spectroradiometer, such that the spectral radiance projected into each pixel of the hyperspectral imager can be accurately known. Use of such projected scenes in a controlled laboratory setting would alleviate expensive field testing of instruments, allow better separation of environmental effects from instrument effects, and enable system-level performance testing and validation of hyperspectral imagers as used with analysis algorithms. For example, known mixtures of relevant endmember spectra could be projected into arbitrary spatial pixels in a hyperspectral imager, enabling tests of how well a full system, consisting of the instrument + calibration + analysis algorithm, performs in unmixing (i.e. de-convolving) the spectra in all pixels. We discuss here the performance of a visible prototype HIP. The technology is readily extendable to the ultraviolet and infrared spectral ranges, and the scenes can be static or dynamic.

EMAAS: An extensible grid-based Rich Internet Application for microarray data analysis and management

PubMed Central

Barton, G; Abbott, J; Chiba, N; Huang, DW; Huang, Y; Krznaric, M; Mack-Smith, J; Saleem, A; Sherman, BT; Tiwari, B; Tomlinson, C; Aitman, T; Darlington, J; Game, L; Sternberg, MJE; Butcher, SA

2008-01-01

Background Microarray experimentation requires the application of complex analysis methods as well as the use of non-trivial computer technologies to manage the resultant large data sets. This, together with the proliferation of tools and techniques for microarray data analysis, makes it very challenging for a laboratory scientist to keep up-to-date with the latest developments in this field. Our aim was to develop a distributed e-support system for microarray data analysis and management. Results EMAAS (Extensible MicroArray Analysis System) is a multi-user rich internet application (RIA) providing simple, robust access to up-to-date resources for microarray data storage and analysis, combined with integrated tools to optimise real time user support and training. The system leverages the power of distributed computing to perform microarray analyses, and provides seamless access to resources located at various remote facilities. The EMAAS framework allows users to import microarray data from several sources to an underlying database, to pre-process, quality assess and analyse the data, to perform functional analyses, and to track data analysis steps, all through a single easy to use web portal. This interface offers distance support to users both in the form of video tutorials and via live screen feeds using the web conferencing tool EVO. A number of analysis packages, including R-Bioconductor and Affymetrix Power Tools have been integrated on the server side and are available programmatically through the Postgres-PLR library or on grid compute clusters. Integrated distributed resources include the functional annotation tool DAVID, GeneCards and the microarray data repositories GEO, CELSIUS and MiMiR. EMAAS currently supports analysis of Affymetrix 3' and Exon expression arrays, and the system is extensible to cater for other microarray and transcriptomic platforms. Conclusion EMAAS enables users to track and perform microarray data management and analysis tasks through a single easy-to-use web application. The system architecture is flexible and scalable to allow new array types, analysis algorithms and tools to be added with relative ease and to cope with large increases in data volume. PMID:19032776

Solar array electrical performance assessment for Space Station Freedom

NASA Technical Reports Server (NTRS)

Smith, Bryan K.; Brisco, Holly

1993-01-01

Electrical power for Space Station Freedom will be generated by large Photovoltaic arrays with a beginning of life power requirement of 30.8 kW per array. The solar arrays will operate in a Low Earth Orbit (LEO) over a design life of fifteen years. This paper provides an analysis of the predicted solar array electrical performance over the design life and presents a summary of supporting analysis and test data for the assigned model parameters and performance loss factors. Each model parameter and loss factor is assessed based upon program requirements, component analysis, and test data to date. A description of the LMSC performance model, future test plans, and predicted performance ranges are also given.

Solar array electrical performance assessment for Space Station Freedom

NASA Technical Reports Server (NTRS)

Smith, Bryan K.; Brisco, Holly

1993-01-01

Electrical power for Space Station Freedom will be generated by large photovoltaic arrays with a beginning of life power requirement of 30.8 kW per array. The solar arrays will operate in a Low Earth Orbit (LEO) over a design life of fifteen years. This paper provides an analysis of the predicted solar array electrical performance over the design life and presents a summary of supporting analysis and test data for the assigned model parameters and performance loss factors. Each model parameter and loss factor is assessed based upon program requirements, component analysis and test data to date. A description of the LMSC performance model future test plans and predicted performance ranges are also given.

The offline combination of thin-layer chromatography and high-performance liquid chromatography with diode array detection and micrOTOF-Q mass spectrometry for the separation and identification of spinochromes from sea urchin (Strongylocentrotus droebachiensis) shells.

PubMed

Shikov, Alexander N; Ossipov, Vladimir I; Martiskainen, Olli; Pozharitskaya, Olga N; Ivanova, Svetlana A; Makarov, Valery G

2011-12-16

Thin-layer chromatography (TLC) with off-line high-performance liquid chromatography coupled to diode array detection and micrOTOF-Q mass spectrometry (HPLC-DAD-MS) resulted in the successful fractionation, separation and identification of spinochrome pigments from sea urchin (Strongylocentrotus droebachiensis) shells. Two fractions of pigments were separated by TLC and eluted with methanol using a TLC-MS interface. HPLC-DAD-MS analysis of the fractions indicated the presence of six sea urchin pigments: spinochrome monomers B and D, three spinochrome dimers (anhydroethylidene-6,6'-bis(2,3,7-trihydroxynaphthazarin) and its isomer and ethylidene-6,6'-bis(2,3,7-trihydroxynaphthazarin)), and one pigment that was preliminary identified as a spinochrome dimer with the structural formula C(22)H(16)O(16). Copyright © 2011 Elsevier B.V. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yulaev, Alexander; Guo, Hongxuan; Strelcov, Evgheni

Atomic-scale thickness, molecular impermeability, low atomic number, and mechanical strength make graphene an ideal electron-transparent membrane for material characterization in liquids and gases with scanning electron microscopy and spectroscopy. Here in this paper, we present a novel sample platform made of an array of thousands of identical isolated graphene-capped microchannels with high aspect ratio. A combination of a global wide field of view with high resolution local imaging of the array allows for high throughput in situ studies as well as for combinatorial screening of solutions, liquid interfaces, and immersed samples. We demonstrate the capabilities of this platform by studyingmore » a pure water sample in comparison with alkali halide solutions, a model electrochemical plating process, and beam-induced crystal growth in liquid electrolyte. Spectroscopic characterization of liquid interfaces and immersed objects with Auger and X-ray fluorescence analysis through the graphene membrane are also demonstrated.« less

Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System.

PubMed

Qu, Xiangmeng; Li, Min; Zhang, Hongbo; Lin, Chenglie; Wang, Fei; Xiao, Mingshu; Zhou, Yi; Shi, Jiye; Aldalbahi, Ali; Pei, Hao; Chen, Hong; Li, Li

2017-09-20

The development of a real-time continuous analytical platform for the pathogen detection is of great scientific importance for achieving better disease control and prevention. In this work, we report a rapid and recyclable microfluidic bioassay system constructed from oligonucleotide arrays for selective and sensitive continuous identification of DNA targets of fungal pathogens. We employ the thermal denaturation method to effectively regenerate the oligonucleotide arrays for multiple sample detection, which could considerably reduce the screening effort and costs. The combination of thermal denaturation and laser-induced fluorescence detection technique enables real-time continuous identification of multiple samples (<10 min per sample). As a proof of concept, we have demonstrated that two DNA targets of fungal pathogens (Botrytis cinerea and Didymella bryoniae) can be sequentially analyzed using our rapid microfluidic bioassay system, which provides a new paradigm in the design of microfluidic bioassay system and will be valuable for chemical and biomedical analysis.

Multipole surface plasmons in metallic nanohole arrays

NASA Astrophysics Data System (ADS)

Nishida, Munehiro; Hatakenaka, Noriyuki; Kadoya, Yutaka

2015-06-01

The quasibound electromagnetic modes for the arrays of nanoholes perforated in thin gold film are analyzed both numerically by the rigorous coupled wave analysis (RCWA) method and semianalytically by the coupled mode method. It is shown that when the size of the nanohole occupies a large portion of the unit cell, the surface plasmon polaritons (SPPs) at both sides of the film are combined by the higher order waveguide modes of the holes to produce multipole surface plasmons: coupled surface plasmon modes with multipole texture on the elec-tric field distributions. Further, it is revealed that the multipole texture either enhances or suppresses the couplings between SPPs depending on their diffraction orders and also causes band inversion and reconstruction in the coupled SPP band structure. Due to the multipole nature of the quasibound modes, multiple dark modes coexist to produce a variety of Fano resonance structures on the transmission and reflection spectra.

Evaluating focused ion beam patterning for position-controlled nanowire growth using computer vision

NASA Astrophysics Data System (ADS)

Mosberg, A. B.; Myklebost, S.; Ren, D.; Weman, H.; Fimland, B. O.; van Helvoort, A. T. J.

2017-09-01

To efficiently evaluate the novel approach of focused ion beam (FIB) direct patterning of substrates for nanowire growth, a reference matrix of hole arrays has been used to study the effect of ion fluence and hole diameter on nanowire growth. Self-catalyzed GaAsSb nanowires were grown using molecular beam epitaxy and studied by scanning electron microscopy (SEM). To ensure an objective analysis, SEM images were analyzed with computer vision to automatically identify nanowires and characterize each array. It is shown that FIB milling parameters can be used to control the nanowire growth. Lower ion fluence and smaller diameter holes result in a higher yield (up to 83%) of single vertical nanowires, while higher fluence and hole diameter exhibit a regime of multiple nanowires. The catalyst size distribution and placement uniformity of vertical nanowires is best for low-value parameter combinations, indicating how to improve the FIB parameters for positioned-controlled nanowire growth.

A neural approach for improving the measurement capability of an electronic nose

NASA Astrophysics Data System (ADS)

Chimenti, M.; DeRossi, D.; Di Francesco, F.; Domenici, C.; Pieri, G.; Pioggia, G.; Salvetti, O.

2003-06-01

Electronic noses, instruments for automatic recognition of odours, are typically composed of an array of partially selective sensors, a sampling system, a data acquisition device and a data processing system. For the purpose of evaluating the quality of olive oil, an electronic nose based on an array of conducting polymer sensors capable of discriminating olive oil aromas was developed. The selection of suitable pattern recognition techniques for a particular application can enhance the performance of electronic noses. Therefore, an advanced neural recognition algorithm for improving the measurement capability of the device was designed and implemented. This method combines multivariate statistical analysis and a hierarchical neural-network architecture based on self-organizing maps and error back-propagation. The complete system was tested using samples composed of characteristic olive oil aromatic components in refined olive oil. The results obtained have shown that this approach is effective in grouping aromas into different categories representative of their chemical structure.

Coherent beam combining architectures for high power tapered laser arrays

NASA Astrophysics Data System (ADS)

Schimmel, G.; Janicot, S.; Hanna, M.; Decker, J.; Crump, P.; Erbert, G.; Witte, U.; Traub, M.; Georges, P.; Lucas-Leclin, G.

2017-02-01

Coherent beam combining (CBC) aims at increasing the spatial brightness of lasers. It consists in maintaining a constant phase relationship between different emitters, in order to combine them constructively in one single beam. We have investigated the CBC of an array of five individually-addressable high-power tapered laser diodes at λ = 976 nm, in two architectures: the first one utilizes the self-organization of the lasers in an interferometric extended-cavity, which ensures their mutual coherence; the second one relies on the injection of the emitters by a single-frequency laser diode. In both cases, the coherent combining of the phase-locked beams is ensured on the front side of the array by a transmission diffractive grating with 98% efficiency. The passive phase-locking of the laser bar is obtained up to 5 A (per emitter). An optimization algorithm is implemented to find the proper currents in the five ridge sections that ensured the maximum combined power on the front side. Under these conditions we achieve a maximum combined power of 7.5 W. In the active MOPA configuration, we can increase the currents in the tapered sections up to 6 A and get a combined power of 11.5 W, corresponding to a combining efficiency of 76%. It is limited by the beam quality of the tapered emitters and by fast phase fluctuations between emitters. Still, these results confirm the potential of CBC approaches with tapered lasers to provide a high-power and high-brightness beam, and compare with the current state-of-the-art with laser diodes.

Wirelessly Networked Digital Phased Array: Analysis and Development of a Phase Synchronization Concept

DTIC Science & Technology

2007-09-01

NAVAL POSTGRADUATE SCHOOL MONTEREY, CALIFORNIA THESIS Approved for public release; distribution is unlimited WIRELESSLY NETWORKED...DIGITAL PHASED ARRAY: ANALYSIS AND DEVELOPMENT OF A PHASE SYNCHRONIZATION CONCEPT by Micael Grahn September 2007 Thesis Advisor...September 2007 3. REPORT TYPE AND DATES COVERED Master’s Thesis 4. TITLE AND SUBTITLE Wirelessly Networked Digital Phased Array: Analysis and

Slotted Polyimide-Aerogel-Filled-Waveguide Arrays

NASA Technical Reports Server (NTRS)

Rodriguez-Solis, Rafael A.; Pacheco, Hector L.; Miranda, Felix A.; Meador, Mary Ann B.

2013-01-01

This presentation discussed the potential advantages of developing Slotted Waveguide Arrays using polyimide aerogels. Polyimide (PI) aerogels offer great promise as an enabling technology for lightweight aerospace antenna systems. PI aerogels are highly porous solids possessing low density and low dielectric permittivity combined with good mechanical properties. For slotted waveguide array applications, there are significant advantages in mass that more than compensate for the slightly higher loss of the aerogel filled waveguide when compared to state of practice commercial waveguide.

Electrostatic Discharge Test of Multi-Junction Solar Array Coupons After Combined Space Environmental Exposures

NASA Technical Reports Server (NTRS)

Wright, Kenneth H.; Schneider, Todd; Vaughn, Jason; Hoang, Bao; Funderburk, Victor V.; Wong, Frankie; Gardiner, George

2010-01-01

A set of multi-junction GaAs/Ge solar array test coupons were subjected to a sequence of 5-year increments of combined environmental exposure tests. The test coupons capture an integrated design intended for use in a geosynchronous (GEO) space environment. A key component of this test campaign is conducting electrostatic discharge (ESD) tests in the inverted gradient mode. The protocol of the ESD tests is based on the ISO/CD 11221, the ISO standard for ESD testing on solar array panels. This standard is currently in its final review with expected approval in 2010. The test schematic in the ISO reference has been modified with Space System/Loral designed circuitry to better simulate the on-orbit operational conditions of its solar array design. Part of the modified circuitry is to simulate a solar array panel coverglass flashover discharge. All solar array coupons used in the test campaign consist of 4 cells. The ESD tests are performed at the beginning of life (BOL) and at each 5-year environment exposure point. The environmental exposure sequence consists of UV radiation, electron/proton particle radiation, thermal cycling, and ion thruster plume. This paper discusses the coverglass flashover simulation, ESD test setup, and the importance of the electrical test design in simulating the on-orbit operational conditions. Results from 5th-year testing are compared to the baseline ESD characteristics determined at the BOL condition.

Add-drop double bus microresonator array local oscillators for sharp multiple Fano resonance engineering

NASA Astrophysics Data System (ADS)

Li, Jiahua; Qu, Ye; Wu, Ying

2018-03-01

Asymmetric resonances are currently the subject of considerable research efforts in photonic nanostructures. Here we propose a feasible method to achieve multiple Fano resonances and their control in an optical compound system consisting of an array of on-chip microresonators without mutual coupling and two parallel fiber waveguides side-coupled to the microresonator array by means of a local oscillator. We derive analytical and transparent expressions for the power transmission function summing over the two light transporting paths within the framework of quantum optics. It is clearly shown that introducing the local oscillator as an additional light propagating path plays an important role in the formation of narrow and multiple Fano resonance lineshapes. The power transmission spectrum through the combination of both the microresonator array and the local oscillator is very sensitive to the system parameters, for example, the intrinsic decay rate of the resonator, the phase shift factor of the local oscillator, the transmission coefficient of the fiber beam splitter, and the total number of the microresonators. Through detailed analysis, we identify the optimums for generating Fano resonance lineshapes. Also, we assess the experimental feasibility of the scheme using currently available technology. The proposed method is relatively straightforward as it requires only one local oscillator as one interferometer arm and it is mostly fiber-based. We believe that our work will help to understand and improve multiple Fano resonance engineering.

Optimisation of the conditions for stripping voltammetric analysis at liquid-liquid interfaces supported at micropore arrays: a computational simulation.

PubMed

Strutwolf, Jörg; Arrigan, Damien W M

2010-10-01

Micropore membranes have been used to form arrays of microinterfaces between immiscible electrolyte solutions (µITIES) as a basis for the sensing of non-redox-active ions. Implementation of stripping voltammetry as a sensing method at these arrays of µITIES was applied recently to detect drugs and biomolecules at low concentrations. The present study uses computational simulation to investigate the optimum conditions for stripping voltammetric sensing at the µITIES array. In this scenario, the diffusion of ions in both the aqueous and the organic phases contributes to the sensing response. The influence of the preconcentration time, the micropore aspect ratio, the location of the microinterface within the pore, the ratio of the diffusion coefficients of the analyte ion in the organic and aqueous phases, and the pore wall angle were investigated. The simulations reveal that the accessibility of the microinterfaces during the preconcentration period should not be hampered by a recessed interface and that diffusional transport in the phase where the analyte ions are preconcentrated should be minimized. This will ensure that the ions are accumulated within the micropores close to the interface and thus be readily available for back transfer during the stripping process. On the basis of the results, an optimal combination of the examined parameters is proposed, which together improve the stripping voltammetric signal and provide an improvement in the detection limit.

A 7T Spine Array Based on Electric Dipole Transmitters

PubMed Central

Duan, Qi; Nair, Govind; Gudino, Natalia; de Zwart, Jacco A.; van Gelderen, Peter; Murphy-Boesch, Joe; Reich, Daniel S.; Duyn, Jeff H.; Merkle, Hellmut

2015-01-01

Purpose In this work the feasibility of using an array of electric dipole antennas for RF transmission in spine MRI at high field is explored. Method A 2-channel transmit array based on an electric dipole design was quantitatively optimized for 7T spine imaging and integrated with a receive array combining 8 loop coils. Using B1+ mapping, the transmit efficiency of the dipole array was compared to a design using quadrature loop pairs. The radio-frequency (RF) energy deposition for each array was measured using a home-built dielectric phantom and MR thermometry. The performance of the proposed array was qualitatively demonstrated in human studies. Results The results indicate dramatically improved transmit efficiency for the dipole design as compared to the loop excitation. Up to 76% gain was achieved within the spinal region. Conclusion For imaging of the spine, electric-dipole based transmitters provided an attractive alternative to the traditional loop-based design. Easy integration with existing receive array technology facilitates practical use at high field. PMID:26190585

Polar-interferometry: what can be learnt from the IOTA/IONIC experiment

NASA Astrophysics Data System (ADS)

Le Bouquin, Jean-Baptiste; Rousselet-Perraut, Karine; Berger, Jean-Philippe; Herwats, Emilie; Benisty, Myriam; Absil, Olivier; Defrere, Denis; Monnier, John; Traub, Wesley

2008-07-01

We report the first near-IR polar-interferometric observations, performed at the IOTA array using its integrated optics combiner IONIC. Fringes have been obtained on calibration stars and resolved late-type giants. Optical modeling of the array and dedicated laboratory measures allowed us to confirm the good accuracy obtained on the calibrated polarized visibilities and closure phases. However, no evidences for polarimetric features at high angular resolution have been detected. The simulations and the results presented here open several perspectives for polar-interferometry, especially in the context of fibered, single-mode combiners.

Temporal compressive sensing systems

DOEpatents

Reed, Bryan W.

2017-12-12

Methods and systems for temporal compressive sensing are disclosed, where within each of one or more sensor array data acquisition periods, one or more sensor array measurement datasets comprising distinct linear combinations of time slice data are acquired, and where mathematical reconstruction allows for calculation of accurate representations of the individual time slice datasets.

At-TAX: a whole genome tiling array resource for developmental expression analysis and transcript identification in Arabidopsis thaliana

PubMed Central

Laubinger, Sascha; Zeller, Georg; Henz, Stefan R; Sachsenberg, Timo; Widmer, Christian K; Naouar, Naïra; Vuylsteke, Marnik; Schölkopf, Bernhard; Rätsch, Gunnar; Weigel, Detlef

2008-01-01

Gene expression maps for model organisms, including Arabidopsis thaliana, have typically been created using gene-centric expression arrays. Here, we describe a comprehensive expression atlas, Arabidopsis thaliana Tiling Array Express (At-TAX), which is based on whole-genome tiling arrays. We demonstrate that tiling arrays are accurate tools for gene expression analysis and identified more than 1,000 unannotated transcribed regions. Visualizations of gene expression estimates, transcribed regions, and tiling probe measurements are accessible online at the At-TAX homepage. PMID:18613972

An Approach for Smart Antenna Testbed

NASA Astrophysics Data System (ADS)

Kawitkar, R. S.; Wakde, D. G.

2003-07-01

The use of wireless, mobile, personal communications services are expanding rapidly. Adaptive or "Smart" antenna arrays can increase channel capacity through spatial division. Adaptive antennas can also track mobile users, improving both signal range and quality. For these reasons, smart antenna systems have attracted widespread interest in the telecommunications industry for applications to third generation wireless systems.This paper aims to design and develop an advanced antennas testbed to serve as a common reference for testing adaptive antenna arrays and signal combining algorithms, as well as complete systems. A flexible suite of off line processing software should be written using matlab to perform system calibration, test bed initialization, data acquisition control, data storage/transfer, off line signal processing and analysis and graph plotting. The goal of this paper is to develop low complexity smart antenna structures for 3G systems. The emphasis will be laid on ease of implementation in a multichannel / multi-user environment. A smart antenna test bed will be developed, and various state-of-the-art DSP structures and algorithms will be investigated.Facing the soaring demand for mobile communications, the use of smart antenna arrays in mobile communications systems to exploit spatial diversity to further improve spectral efficiency has recently received considerable attention. Basically, a smart antenna array comprises a number of antenna elements combined via a beamforming network (amplitude and phase control network). Some of the benefits that can be achieved by using SAS (Smart Antenna System) include lower mobile terminal power consumption, range extension, ISI reduction, higher data rate support, and ease of integration into the existing base station system. In terms of economic benefits, adaptive antenna systems employed at base station, though increases the per base station cost, can increase coverage area of each cell site, thereby reducing the total system cost dramatically - often by more than 50% without compromising the system performance. The testbed can be employed to illustrate enhancement of system capacity and service quality in wireless communications.

A Novel Hybrid Classification Model of Genetic Algorithms, Modified k-Nearest Neighbor and Developed Backpropagation Neural Network

PubMed Central

Salari, Nader; Shohaimi, Shamarina; Najafi, Farid; Nallappan, Meenakshii; Karishnarajah, Isthrinayagy

2014-01-01

Among numerous artificial intelligence approaches, k-Nearest Neighbor algorithms, genetic algorithms, and artificial neural networks are considered as the most common and effective methods in classification problems in numerous studies. In the present study, the results of the implementation of a novel hybrid feature selection-classification model using the above mentioned methods are presented. The purpose is benefitting from the synergies obtained from combining these technologies for the development of classification models. Such a combination creates an opportunity to invest in the strength of each algorithm, and is an approach to make up for their deficiencies. To develop proposed model, with the aim of obtaining the best array of features, first, feature ranking techniques such as the Fisher's discriminant ratio and class separability criteria were used to prioritize features. Second, the obtained results that included arrays of the top-ranked features were used as the initial population of a genetic algorithm to produce optimum arrays of features. Third, using a modified k-Nearest Neighbor method as well as an improved method of backpropagation neural networks, the classification process was advanced based on optimum arrays of the features selected by genetic algorithms. The performance of the proposed model was compared with thirteen well-known classification models based on seven datasets. Furthermore, the statistical analysis was performed using the Friedman test followed by post-hoc tests. The experimental findings indicated that the novel proposed hybrid model resulted in significantly better classification performance compared with all 13 classification methods. Finally, the performance results of the proposed model was benchmarked against the best ones reported as the state-of-the-art classifiers in terms of classification accuracy for the same data sets. The substantial findings of the comprehensive comparative study revealed that performance of the proposed model in terms of classification accuracy is desirable, promising, and competitive to the existing state-of-the-art classification models. PMID:25419659

Preliminary Design of a Manned Nuclear Electric Propulsion Vehicle Using Genetic Algorithms

NASA Technical Reports Server (NTRS)

Irwin, Ryan W.; Tinker, Michael L.

2005-01-01

Nuclear electric propulsion (NEP) vehicles will be needed for future manned missions to Mars and beyond. Candidate designs must be identified for further detailed design from a large array of possibilities. Genetic algorithms have proven their utility in conceptual design studies by effectively searching a large design space to pinpoint unique optimal designs. This research combined analysis codes for NEP subsystems with a genetic algorithm. The use of penalty functions with scaling ratios was investigated to increase computational efficiency. Also, the selection of design variables for optimization was considered to reduce computation time without losing beneficial design search space. Finally, trend analysis of a reference mission to the asteroids yielded a group of candidate designs for further analysis.

Temporal discounting: basic research and the analysis of socially important behavior.

PubMed Central

Critchfield, T S; Kollins, S H

2001-01-01

Recent basic research on human temporal discounting is reviewed to illustrate procedures, summarize key findings, and draw parallels with both nonhuman animal research and conceptual writings on self-control. Lessons derived from this research are then applied to the challenge of analyzing socially important behaviors such as drug abuse, eating and exercise, and impulsiveness associated with attention deficit hyperactivity disorder. Attending to the broader temporal context in which behavior occurs may aid in the analysis of socially important behavior. Applying this perspective to the study of behavior in natural environments also highlights the importance of combining methodological flexibility with conceptual rigor to promote the extension of applied behavior analysis to a broader array of socially important behaviors. PMID:11317983

Automated array assembly task, phase 1

NASA Technical Reports Server (NTRS)

Carbajal, B. G.

1977-01-01

An assessment of state-of-the-art technologies that are applicable to silicon solar cell and solar cell module fabrication is provided. The assessment consists of a technical feasibility evaluation and a cost projection for high-volume production of silicon solar cell modules. The cost projection was approached from two directions; a design-to-cost analysis assigned cost goals to each major process element in the fabrication scheme, and a cost analysis built up projected costs for alternate technologies for each process element. A technical evaluation was used in combination with the cost analysis to identify a baseline low cost process. A novel approach to metal pattern design based on minimum power loss was developed. These design equations were used as a tool in the evaluation of metallization technologies.

A new type of recording chamber with an easy-to-exchange microdrive array for chronic recordings in macaque monkeys.

PubMed

Galashan, F Orlando; Rempel, Hanna C; Meyer, Anneke; Gruber-Dujardin, Eva; Kreiter, Andreas K; Wegener, Detlef

2011-06-01

In monkeys, long-term recordings with chronically implanted microelectrodes frequently suffer from a continuously decreasing probability to record single units or even small multiunit clusters. This problem is associated with two technical limitations of the available devices: first, restrictions for electrode movement, and second, absent possibility to exchange electrodes easily on a regular basis. Permitting to adjust the recording site and to use new recording tracks with proper electrodes may avoid these problems and make chronic more similar to acute recordings. Here, we describe a novel type of implant tackling this issue. It consists of a new type of recording chamber combined with an exchangeable multielectrode array that precisely fits into it. The multielectrode array is reversibly fixed to the chamber, and within a minute it can be exchanged against another array equipped with new electrodes at the awake animal. The array allows for bidirectional movement of six electrodes for a distance of up to 12 mm. The recording chamber enables hermetical isolation of the intracranial space, resulting in long-lasting aseptic conditions and reducing dural thickening to a minimum, as confirmed by microbiological and histopathological analysis. The device has a simple design and is both easy to produce and low in cost. Functionality has been tested in primary and secondary visual cortex of three macaque monkeys over a period of up to 15 mo. The results show that even after more than a year, single and multiunit responses can be obtained with high incidence.

The Use of a Pressure-Indicating Sensor Film to Provide Feedback upon Hydrogel-Forming Microneedle Array Self-Application In Vivo.

PubMed

Vicente-Pérez, Eva M; Quinn, Helen L; McAlister, Emma; O'Neill, Shannon; Hanna, Lezley-Anne; Barry, Johanne G; Donnelly, Ryan F

2016-12-01

To evaluate the combination of a pressure-indicating sensor film with hydrogel-forming microneedle arrays, as a method of feedback to confirm MN insertion in vivo. Pilot in vitro insertion studies were conducted using a Texture Analyser to insert MN arrays, coupled with a pressure-indicating sensor film, at varying forces into excised neonatal porcine skin. In vivo studies involved twenty human volunteers, who self-applied two hydrogel-forming MN arrays, one with a pressure-indicating sensor film incorporated and one without. Optical coherence tomography was employed to measure the resulting penetration depth and colorimetric analysis to investigate the associated colour change of the pressure-indicating sensor film. Microneedle insertion was achieved in vitro at three different forces, demonstrating the colour change of the pressure-indicating sensor film upon application of increasing pressure. When self-applied in vivo, there was no significant difference in the microneedle penetration depth resulting from each type of array, with a mean depth of 237 μm recorded. When the pressure-indicating sensor film was present, a colour change occurred upon each application, providing evidence of insertion. For the first time, this study shows how the incorporation of a simple, low-cost pressure-indicating sensor film can indicate microneedle insertion in vitro and in vivo, providing visual feedback to assure the user of correct application. Such a strategy may enhance usability of a microneedle device and, hence, assist in the future translation of the technology to widespread clinical use.

Integrated focal plane arrays for millimeter-wave astronomy

NASA Astrophysics Data System (ADS)

Bock, James J.; Goldin, Alexey; Hunt, Cynthia; Lange, Andrew E.; Leduc, Henry G.; Day, Peter K.; Vayonakis, Anastasios; Zmuidzinas, Jonas

2002-02-01

We are developing focal plane arrays of bolometric detectors for sub-millimeter and millimeter-wave astrophysics. We propose a flexible array architecture using arrays of slot antennae coupled via low-loss superconducting Nb transmission line to microstrip filters and antenna-coupled bolometers. By combining imaging and filtering functions with transmission line, we are able to realize unique structures such as a multi-band polarimeter and a planar, dispersive spectrometer. Micro-strip bolometers have significantly smaller active volume than standard detectors with extended absorbers, and can realize higher sensitivity and speed of response. The integrated array has natural immunity to stray radiation or spectral leaks, and minimizes the suspended mass operating at 0.1-0.3 K. We also discuss future space-borne spectroscopy and polarimetry applications. .

Integrated dynamic analysis simulation of space stations with controllable solar arrays (supplemental data and analyses)

NASA Technical Reports Server (NTRS)

Heinrichs, J. A.; Fee, J. J.

1972-01-01

Space station and solar array data and the analyses which were performed in support of the integrated dynamic analysis study. The analysis methods and the formulated digital simulation were developed. Control systems for space station altitude control and solar array orientation control include generic type control systems. These systems have been digitally coded and included in the simulation.

Combining mouse mammary gland gene expression and comparative mapping for the identification of candidate genes for QTL of milk production traits in cattle

PubMed Central

Ron, Micha; Israeli, Galit; Seroussi, Eyal; Weller, Joel I; Gregg, Jeffrey P; Shani, Moshe; Medrano, Juan F

2007-01-01

Background Many studies have found segregating quantitative trait loci (QTL) for milk production traits in different dairy cattle populations. However, even for relatively large effects with a saturated marker map the confidence interval for QTL location by linkage analysis spans tens of map units, or hundreds of genes. Combining mapping and arraying has been suggested as an approach to identify candidate genes. Thus, gene expression analysis in the mammary gland of genes positioned in the confidence interval of the QTL can bridge the gap between fine mapping and quantitative trait nucleotide (QTN) determination. Results We hybridized Affymetrix microarray (MG-U74v2), containing 12,488 murine probes, with RNA derived from mammary gland of virgin, pregnant, lactating and involuting C57BL/6J mice in a total of nine biological replicates. We combined microarray data from two additional studies that used the same design in mice with a total of 75 biological replicates. The same filtering and normalization was applied to each microarray data using GeneSpring software. Analysis of variance identified 249 differentially expressed probe sets common to the three experiments along the four developmental stages of puberty, pregnancy, lactation and involution. 212 genes were assigned to their bovine map positions through comparative mapping, and thus form a list of candidate genes for previously identified QTLs for milk production traits. A total of 82 of the genes showed mammary gland-specific expression with at least 3-fold expression over the median representing all tissues tested in GeneAtlas. Conclusion This work presents a web tool for candidate genes for QTL (cgQTL) that allows navigation between the map of bovine milk production QTL, potential candidate genes and their level of expression in mammary gland arrays and in GeneAtlas. Three out of four confirmed genes that affect QTL in livestock (ABCG2, DGAT1, GDF8, IGF2) were over expressed in the target organ. Thus, cgQTL can be used to determine priority of candidate genes for QTN analysis based on differential expression in the target organ. PMID:17584498

All-silicon nanorod-based Dammann gratings.

PubMed

Li, Zile; Zheng, Guoxing; He, Ping'An; Li, Song; Deng, Qiling; Zhao, Jiangnan; Ai, Yong

2015-09-15

Established diffractive optical elements (DOEs), such as Dammann gratings, whose phase profile is controlled by etching different depths into a transparent dielectric substrate, suffer from a contradiction between the complexity of fabrication procedures and the performance of such gratings. In this Letter, we combine the concept of geometric phase and phase modulation in depth, and prove by theoretical analysis and numerical simulation that nanorod arrays etched on a silicon substrate have a characteristic of strong polarization conversion between two circularly polarized states and can act as a highly efficient half-wave plate. More importantly, only by changing the orientation angles of each nanorod can the arrays control the phase of a circularly polarized light, cell by cell. With the above principle, we report the realization of nanorod-based Dammann gratings reaching diffraction efficiencies of 50%-52% in the C-band fiber telecommunications window (1530-1565 nm). In this design, uniform 4×4 spot arrays with an extending angle of 59°×59° can be obtained in the far field. Because of these advantages of the single-step fabrication procedure, accurate phase controlling, and strong polarization conversion, nanorod-based Dammann gratings could be utilized for various practical applications in a range of fields.

Switched-beam radiometer front-end network analysis

NASA Technical Reports Server (NTRS)

Trew, R. J.; Bilbro, G. L.

1994-01-01

The noise figure performance of various delay-line networks fabricated from microstrip lines with varying number of elements was investigated using a computer simulation. The effects of resistive losses in both the transmission lines and power combiners were considered. In general, it is found that an optimum number of elements exists, depending upon the resistive losses present in the network. Small resistive losses are found to have a significant degrading effect upon the noise figure performance of the array. Extreme stability in switching characteristics is necessary to minimize the nondeterministic noise of the array. For example, it is found that a 6 percent tolerance on the delay-line lengths will produce a 0.2 db uncertainty in the noise figure which translates into a 13.67 K temperature uncertainty generated by the network. If the tolerance can be held to 2 percent, the uncertainty in noise figure and noise temperature will be 0.025 db and 1.67 K, respectively. Three phase shift networks fabricated using a commercially available PIN diode switch were investigated. Loaded-line phase shifters are found to have desirable RF and noise characteristics and are attractive components for use in phased-array networks.

A combined microphone and camera calibration technique with application to acoustic imaging.

PubMed

Legg, Mathew; Bradley, Stuart

2013-10-01

We present a calibration technique for an acoustic imaging microphone array, combined with a digital camera. Computer vision and acoustic time of arrival data are used to obtain microphone coordinates in the camera reference frame. Our new method allows acoustic maps to be plotted onto the camera images without the need for additional camera alignment or calibration. Microphones and cameras may be placed in an ad-hoc arrangement and, after calibration, the coordinates of the microphones are known in the reference frame of a camera in the array. No prior knowledge of microphone positions, inter-microphone spacings, or air temperature is required. This technique is applied to a spherical microphone array and a mean difference of 3 mm was obtained between the coordinates obtained with this calibration technique and those measured using a precision mechanical method.

The Improved Power of the Central Lobe in the Beam Combination and High Power Output

NASA Astrophysics Data System (ADS)

Liu, Hou-Kang; Xue, Yu-Hao; Li, Zhen; He, Bing; Zhou, Jun; Ding, Ya-Qian; Jiao, Meng-Li; Liu, Chi; Qi, Yun-Feng; Wei, Yun-Rong; Dong, Jing-Xing; Lou, Qi-Hong

2012-04-01

In order to increase the power fraction of the central lobe in the coherent beam combination of lasers in an array, the effects of the distance factor of near-field distribution on far-field interference patterns are calculated and demonstrated experimentally. An improved beam array of interwoven distribution is demonstrated to enable the power in the central lobe to reach 41%. An optimized mirror array is carefully designed to obtain a high duty ratio, which is up to 53.3% at a high power level. By using these optimized methods and designs, the passive phase locking of eight Yb-doped fiber amplifiers with ring cavities are obtained, and a pleasing interference pattern with 87% visibility is observed. The maximum coherent output power of the system is up to 1066 W.

Imaging with New Classic and Vision at the NPOI

NASA Astrophysics Data System (ADS)

Jorgensen, Anders

2018-04-01

The Navy Precision Optical Interferometer (NPOI) is unique among interferometric observatories for its ability to position telescopes in an equally-spaced array configuration. This configuration is optimal for interferometric imaging because it allows the use of bootstrapping to track fringes on long baselines with signal-to-noise ratio less than one. When combined with coherent integration techniques this can produce visibilities with acceptable SNR on baselines long enough to resolve features on the surfaces of stars. The stellar surface imaging project at NPOI combines the bootstrapping array configuration of the NPOI array, real-time fringe tracking, baseline- and wavelength bootstrapping with Earth rotation to provide dense coverage in the UV plane at a wide range of spatial frequencies. In this presentation, we provide an overview of the project and an update of the latest status and results from the project.

GACT: a Genome build and Allele definition Conversion Tool for SNP imputation and meta-analysis in genetic association studies.

PubMed

Sulovari, Arvis; Li, Dawei

2014-07-19

Genome-wide association studies (GWAS) have successfully identified genes associated with complex human diseases. Although much of the heritability remains unexplained, combining single nucleotide polymorphism (SNP) genotypes from multiple studies for meta-analysis will increase the statistical power to identify new disease-associated variants. Meta-analysis requires same allele definition (nomenclature) and genome build among individual studies. Similarly, imputation, commonly-used prior to meta-analysis, requires the same consistency. However, the genotypes from various GWAS are generated using different genotyping platforms, arrays or SNP-calling approaches, resulting in use of different genome builds and allele definitions. Incorrect assumptions of identical allele definition among combined GWAS lead to a large portion of discarded genotypes or incorrect association findings. There is no published tool that predicts and converts among all major allele definitions. In this study, we have developed a tool, GACT, which stands for Genome build and Allele definition Conversion Tool, that predicts and inter-converts between any of the common SNP allele definitions and between the major genome builds. In addition, we assessed several factors that may affect imputation quality, and our results indicated that inclusion of singletons in the reference had detrimental effects while ambiguous SNPs had no measurable effect. Unexpectedly, exclusion of genotypes with missing rate > 0.001 (40% of study SNPs) showed no significant decrease of imputation quality (even significantly higher when compared to the imputation with singletons in the reference), especially for rare SNPs. GACT is a new, powerful, and user-friendly tool with both command-line and interactive online versions that can accurately predict, and convert between any of the common allele definitions and between genome builds for genome-wide meta-analysis and imputation of genotypes from SNP-arrays or deep-sequencing, particularly for data from the dbGaP and other public databases. http://www.uvm.edu/genomics/software/gact.

The Air Force concentrating photovoltaic array program

NASA Technical Reports Server (NTRS)

Geis, Jack W.

1987-01-01

A summary is given of Air Force solar concentrator projects beginning with the Rockwell International study program in 1977. The Satellite Materials Hardening Programs (SMATH) explored and developed techniques for hardening planar solar cell array power systems to the combined nuclear and laser radiation threat environments. A portion of program dollars was devoted to developing a preliminary design for a hardened solar concentrator. The results of the Survivable Concentrating Photovoltaic Array (SCOPA) program, and the design, fabrication and flight qualification of a hardened concentrator panel are discussed.

Sub-100-nm ordered silicon hole arrays by metal-assisted chemical etching

PubMed Central

2013-01-01

Sub-100-nm silicon nanohole arrays were fabricated by a combination of the site-selective electroless deposition of noble metals through anodic porous alumina and the subsequent metal-assisted chemical etching. Under optimum conditions, the formation of deep straight holes with an ordered periodicity (e.g., 100 nm interval, 40 nm diameter, and high aspect ratio of 50) was successfully achieved. By using the present method, the fabrication of silicon nanohole arrays with 60-nm periodicity was also achieved. PMID:24090268

Studies of air showers produced by primaries 10(16) eV using a combined scintillation and water-Cerenkov array

NASA Technical Reports Server (NTRS)

Brooke, G.; Perrett, J. C.; Watson, A. A.

1986-01-01

An array of 8 x 1.0 sq m plastic scintillation counters and 13 water-Cerenkov detectors (1 to 13.5 sq m) were operated at the center of the Haverah Park array to study some features of air showers produced by 10(16) eV primaries. Measurements of the scintillator lateral distribution function, the water-Cerenkov lateral distribution function, and of the distance dependence of the Cerenkov/scintillator ratio are described.

Flexible Multiplexed Surface Temperature Sensor

NASA Technical Reports Server (NTRS)

Daryabeigi, Kamran; Dillon-Townes, L. A.; Johnson, Preston B.; Ash, Robert L.

1995-01-01

Unitary array of sensors measures temperatures at points distributed over designated area on surface. Useful in measuring surface temperatures of aerodynamic models and thermally controlled objects. Made of combination of integrated-circuit microchips and film circuitry. Temperature-sensing chips scanned at speeds approaching 10 kHz. Operating range minus 40 degrees C to 120 degrees C. Flexibility of array conforms to curved surfaces. Multiplexer eliminates numerous monitoring cables. Control of acquisition and recording of data effected by connecting array to microcomputers via suitable interface circuitry.

Simultaneous separation of antihyperlipidemic drugs by green ultrahigh-performance liquid chromatography-diode array detector method: Improving the health of liquid chromatography.

PubMed

Alghazi, Mansoor; Alanazi, Fars; Mohsin, Kazi; Siddiqui, Nasir Ali; Shakeel, Faiyaz; Haq, Nazrul

2017-04-01

Statins in combination with fibrates show beneficial effects on the lipoprotein profile of patients because they have positive complimentary effects on lipid profile. A new green ultrahigh-performance liquid chromatography-diode array detector method for simultaneous analysis of simvastatin (SMV) and fenofibrate (FNF) in standard form, marketed formulations, and self-emulsifying drug delivery system formulations was developed and validated in the present investigation. The method utilized C 18 as stationary phase and a combination of methanol:water (8:2) as an eluent. It was found that selected eluent provided short run time (2.5 minutes), better peak symmetry and satisfactory values of other chromatographic parameters such as resolution (Rs=2.325), capacity factor (k, 3.0 and 4.2 for SMV and FNF, respectively), selectivity (α =1.4), and number of theoretical plates (N, 4265 and 5285 for SMV and FNF, respectively). An excellent linear relationship (r 2 0.998 and 0.997 for SMV and FNF, respectively) was observed for linear regression data for the calibration plots. The developed system was validated for accuracy, precision, robustness (˃ 2% for both drugs) and recovery (98-102% for both drugs). Results obtained from the statistical treatment of the values obtained for different parameters proved that the method is suitable, reproducible, and selective for the simultaneous analysis of SMV and FNF in bulk, marketed, and self-emulsifying drug delivery system formulations. The replacement of commonly applied toxic solvents with innocuous and environmentally benign solvents provides a better option than the more toxic processes in drug analysis. Copyright © 2016. Published by Elsevier B.V.

Disturbance characteristics of half-selected cells in a cross-point resistive switching memory array

NASA Astrophysics Data System (ADS)

Chen, Zhe; Li, Haitong; Chen, Hong-Yu; Chen, Bing; Liu, Rui; Huang, Peng; Zhang, Feifei; Jiang, Zizhen; Ye, Hongfei; Gao, Bin; Liu, Lifeng; Liu, Xiaoyan; Kang, Jinfeng; Wong, H.-S. Philip; Yu, Shimeng

2016-05-01

Disturbance characteristics of cross-point resistive random access memory (RRAM) arrays are comprehensively studied in this paper. An analytical model is developed to quantify the number of pulses (#Pulse) the cell can bear before disturbance occurs under various sub-switching voltage stresses based on physical understanding. An evaluation methodology is proposed to assess the disturb behavior of half-selected (HS) cells in cross-point RRAM arrays by combining the analytical model and SPICE simulation. The characteristics of cross-point RRAM arrays such as energy consumption, reliable operating cycles and total error bits are evaluated by the methodology. A possible solution to mitigate disturbance is proposed.

Synthesis and characterization of axial heterojunction inorganic-organic semiconductor nanowire arrays.

PubMed

Chen, Nan; Qian, Xuemin; Lin, Haowei; Liu, Huibiao; Li, Yongjun; Li, Yuliang

2011-11-07

The end-to-end P-N heterojunction nanowire arrays combined organic (poly[1,4-bis(pyrrol-2-yl)benzene], BPB) and inorganic (CdS) molecules have been successfully designed and fabricated. The electrical properties of P-N heterojunctions of organic-inorganic nanowire arrays were investigated. The diode nature and rectifying feature of P-N heterojunction nanowire arrays were observed. The rectification ratio of the diode increased from 29.9 to 129.7 as the illumination intensity increased. The material exhibits a new property, which is an improvement in the integration of the physical and chemical properties of the two independent components.

Geometric analysis and restitution of digital multispectral scanner data arrays

NASA Technical Reports Server (NTRS)

Baker, J. R.; Mikhail, E. M.

1975-01-01

An investigation was conducted to define causes of geometric defects within digital multispectral scanner (MSS) data arrays, to analyze the resulting geometric errors, and to investigate restitution methods to correct or reduce these errors. Geometric transformation relationships for scanned data, from which collinearity equations may be derived, served as the basis of parametric methods of analysis and restitution of MSS digital data arrays. The linearization of these collinearity equations is presented. Algorithms considered for use in analysis and restitution included the MSS collinearity equations, piecewise polynomials based on linearized collinearity equations, and nonparametric algorithms. A proposed system for geometric analysis and restitution of MSS digital data arrays was used to evaluate these algorithms, utilizing actual MSS data arrays. It was shown that collinearity equations and nonparametric algorithms both yield acceptable results, but nonparametric algorithms possess definite advantages in computational efficiency. Piecewise polynomials were found to yield inferior results.

Read margin analysis of crossbar arrays using the cell-variability-aware simulation method

NASA Astrophysics Data System (ADS)

Sun, Wookyung; Choi, Sujin; Shin, Hyungsoon

2018-02-01

This paper proposes a new concept of read margin analysis of crossbar arrays using cell-variability-aware simulation. The size of the crossbar array should be considered to predict the read margin characteristic of the crossbar array because the read margin depends on the number of word lines and bit lines. However, an excessively high-CPU time is required to simulate large arrays using a commercial circuit simulator. A variability-aware MATLAB simulator that considers independent variability sources is developed to analyze the characteristics of the read margin according to the array size. The developed MATLAB simulator provides an effective method for reducing the simulation time while maintaining the accuracy of the read margin estimation in the crossbar array. The simulation is also highly efficient in analyzing the characteristic of the crossbar memory array considering the statistical variations in the cell characteristics.

A systems approach to designing next generation vaccines: combining α-galactose modified antigens with nanoparticle platforms

NASA Astrophysics Data System (ADS)

Phanse, Yashdeep; Carrillo-Conde, Brenda R.; Ramer-Tait, Amanda E.; Broderick, Scott; Kong, Chang Sun; Rajan, Krishna; Flick, Ramon; Mandell, Robert B.; Narasimhan, Balaji; Wannemuehler, Michael J.

2014-01-01

Innovative vaccine platforms are needed to develop effective countermeasures against emerging and re-emerging diseases. These platforms should direct antigen internalization by antigen presenting cells and promote immunogenic responses. This work describes an innovative systems approach combining two novel platforms, αGalactose (αGal)-modification of antigens and amphiphilic polyanhydride nanoparticles as vaccine delivery vehicles, to rationally design vaccine formulations. Regimens comprising soluble αGal-modified antigen and nanoparticle-encapsulated unmodified antigen induced a high titer, high avidity antibody response with broader epitope recognition of antigenic peptides than other regimen. Proliferation of antigen-specific CD4+ T cells was also enhanced compared to a traditional adjuvant. Combining the technology platforms and augmenting immune response studies with peptide arrays and informatics analysis provides a new paradigm for rational, systems-based design of next generation vaccine platforms against emerging and re-emerging pathogens.

Failure mode analysis of silicon-based intracortical microelectrode arrays in non-human primates

PubMed Central

Barrese, James C; Rao, Naveen; Paroo, Kaivon; Triebwasser, Corey; Vargas-Irwin, Carlos; Franquemont, Lachlan; Donoghue, John P

2016-01-01

Objective Brain–computer interfaces (BCIs) using chronically implanted intracortical microelectrode arrays (MEAs) have the potential to restore lost function to people with disabilities if they work reliably for years. Current sensors fail to provide reliably useful signals over extended periods of time for reasons that are not clear. This study reports a comprehensive retrospective analysis from a large set of implants of a single type of intracortical MEA in a single species, with a common set of measures in order to evaluate failure modes. Approach Since 1996, 78 silicon MEAs were implanted in 27 monkeys (Macaca mulatta). We used two approaches to find reasons for sensor failure. First, we classified the time course leading up to complete recording failure as acute (abrupt) or chronic (progressive). Second, we evaluated the quality of electrode recordings over time based on signal features and electrode impedance. Failure modes were divided into four categories: biological, material, mechanical, and unknown. Main results Recording duration ranged from 0 to 2104 days (5.75 years), with a mean of 387 days and a median of 182 days (n = 78). Sixty-two arrays failed completely with a mean time to failure of 332 days (median = 133 days) while nine array experiments were electively terminated for experimental reasons (mean = 486 days). Seven remained active at the close of this study (mean = 753 days). Most failures (56%) occurred within a year of implantation, with acute mechanical failures the most common class (48%), largely because of connector issues (83%). Among grossly observable biological failures (24%), a progressive meningeal reaction that separated the array from the parenchyma was most prevalent (14.5%). In the absence of acute interruptions, electrode recordings showed a slow progressive decline in spike amplitude, noise amplitude, and number of viable channels that predicts complete signal loss by about eight years. Impedance measurements showed systematic early increases, which did not appear to affect recording quality, followed by a slow decline over years. The combination of slowly falling impedance and signal quality in these arrays indicate that insulating material failure is the most significant factor. Significance This is the first long-term failure mode analysis of an emerging BCI technology in a large series of non-human primates. The classification system introduced here may be used to standardize how neuroprosthetic failure modes are evaluated. The results demonstrate the potential for these arrays to record for many years, but achieving reliable sensors will require replacing connectors with implantable wireless systems, controlling the meningeal reaction, and improving insulation materials. These results will focus future research in order to create clinical neuroprosthetic sensors, as well as valuable research tools, that are able to safely provide reliable neural signals for over a decade. PMID:24216311

Failure mode analysis of silicon-based intracortical microelectrode arrays in non-human primates

NASA Astrophysics Data System (ADS)

Barrese, James C.; Rao, Naveen; Paroo, Kaivon; Triebwasser, Corey; Vargas-Irwin, Carlos; Franquemont, Lachlan; Donoghue, John P.

2013-12-01

Objective. Brain-computer interfaces (BCIs) using chronically implanted intracortical microelectrode arrays (MEAs) have the potential to restore lost function to people with disabilities if they work reliably for years. Current sensors fail to provide reliably useful signals over extended periods of time for reasons that are not clear. This study reports a comprehensive retrospective analysis from a large set of implants of a single type of intracortical MEA in a single species, with a common set of measures in order to evaluate failure modes. Approach. Since 1996, 78 silicon MEAs were implanted in 27 monkeys (Macaca mulatta). We used two approaches to find reasons for sensor failure. First, we classified the time course leading up to complete recording failure as acute (abrupt) or chronic (progressive). Second, we evaluated the quality of electrode recordings over time based on signal features and electrode impedance. Failure modes were divided into four categories: biological, material, mechanical, and unknown. Main results. Recording duration ranged from 0 to 2104 days (5.75 years), with a mean of 387 days and a median of 182 days (n = 78). Sixty-two arrays failed completely with a mean time to failure of 332 days (median = 133 days) while nine array experiments were electively terminated for experimental reasons (mean = 486 days). Seven remained active at the close of this study (mean = 753 days). Most failures (56%) occurred within a year of implantation, with acute mechanical failures the most common class (48%), largely because of connector issues (83%). Among grossly observable biological failures (24%), a progressive meningeal reaction that separated the array from the parenchyma was most prevalent (14.5%). In the absence of acute interruptions, electrode recordings showed a slow progressive decline in spike amplitude, noise amplitude, and number of viable channels that predicts complete signal loss by about eight years. Impedance measurements showed systematic early increases, which did not appear to affect recording quality, followed by a slow decline over years. The combination of slowly falling impedance and signal quality in these arrays indicates that insulating material failure is the most significant factor. Significance. This is the first long-term failure mode analysis of an emerging BCI technology in a large series of non-human primates. The classification system introduced here may be used to standardize how neuroprosthetic failure modes are evaluated. The results demonstrate the potential for these arrays to record for many years, but achieving reliable sensors will require replacing connectors with implantable wireless systems, controlling the meningeal reaction, and improving insulation materials. These results will focus future research in order to create clinical neuroprosthetic sensors, as well as valuable research tools, that are able to safely provide reliable neural signals for over a decade.

CAKE: the coincidence array for K600 experiments

NASA Astrophysics Data System (ADS)

Adsley, P.; Neveling, R.; Papka, P.; Dyers, Z.; Brümmer, J. W.; Diget, C. Aa.; Hubbard, N. J.; Li, K. C. W.; Long, A.; Marin-Lambarri, D. J.; Pellegri, L.; Pesudo, V.; Pool, L. C.; Smit, F. D.; Triambak, S.

2017-02-01

The combination of a magnetic spectrometer and ancillary detectors such as silicon detectors is a powerful tool for the study of nuclear reactions and nuclear structure. This paper discusses the recently commissioned silicon array called the "CAKE" which is designed for use with the K600 magnetic spectrometer at iThemba LABS.

Microwave performance characterization of large space antennas

NASA Technical Reports Server (NTRS)

Bathker, D. A. (Editor)

1977-01-01

Performance capabilities of large microwave space antenna configurations with apertures generally from 100 wavelengths upwards are discussed. Types of antennas considered include: phased arrays, lenses, reflectors, and hybrid combinations of phased arrays with reflectors or lenses. The performance characteristics of these broad classes of antennas are examined and compared in terms of applications.

Waveform synthesizer

DOEpatents

Franks, Larry A.; Nelson, Melvin A.

1981-01-01

A method of producing optical and electrical pulses of desired shape. An optical pulse of arbitrary but defined shape illuminates one end of an array of optical fiber waveguides of differing lengths to time differentiate the input pulse. The optical outputs at the other end of the array are combined to form a synthesized pulse of desired shape.

DETERMINATION OF CARBENDAZIM IN WATER BY HIGH-PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY ON-LINE WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE-ARRAY OR MASS SPECTROMETRIC DETECTION

EPA Science Inventory

An automated method for the determination of carbendazim in water that combines high-performance immunoaffinity chromatography (HPIAC), high-performance liquid chromatography (HPLC) in the reversed-phase mode, and detection by either UV-Vis diode array detector (DAD) spectroscopy...

Bulk- and surface-modified combinable PDMS capillary sensor array as an easy-to-use sensing device with enhanced sensitivity to elevated concentrations of multiple serum sample components.

PubMed

Fujii, Yuji; Henares, Terence G; Kawamura, Kunio; Endo, Tatsuro; Hisamoto, Hideaki

2012-04-21

To enhance sensitivity and facilitate easy sample introduction into a combinable poly(dimethylsiloxane) (PDMS) capillary (CPC) sensor array, PDMS was modified in bulk and on its surface to prepare "black" PDMS coated with a silver layer and self-assembled monolayer (SAM). India ink, a traditional Japanese black ink, was added to the PDMS pre-polymer for bulk modification. The surface was modified by a silver mirror reaction followed by SAM formation using cysteine. These modifications enhanced the fluorescence signals by reflecting them from the surface and reducing background interference. A decrease in the water contact angle led to enhanced sensitivity and easy sample introduction. Furthermore, a CPC sensor array for multiplex detection of serum sample components was prepared that could quantify the analytes glucose, potassium, and alkaline phosphatase (ALP). When serum samples were introduced by capillary action, the CPC sensor array showed fluorescence responses for each analyte and successfully identified the components with elevated concentrations in the serum samples.

Fully Tunable Silicon Nanowire Arrays Fabricated by Soft Nanoparticle Templating.

PubMed

Rey, By Marcel; Elnathan, Roey; Ditcovski, Ran; Geisel, Karen; Zanini, Michele; Fernandez-Rodriguez, Miguel-Angel; Naik, Vikrant V; Frutiger, Andreas; Richtering, Walter; Ellenbogen, Tal; Voelcker, Nicolas H; Isa, Lucio

2016-01-13

We demonstrate a fabrication breakthrough to produce large-area arrays of vertically aligned silicon nanowires (VA-SiNWs) with full tunability of the geometry of the single nanowires and of the whole array, paving the way toward advanced programmable designs of nanowire platforms. At the core of our fabrication route, termed "Soft Nanoparticle Templating", is the conversion of gradually compressed self-assembled monolayers of soft nanoparticles (microgels) at a water-oil interface into customized lithographical masks to create VA-SiNW arrays by means of metal-assisted chemical etching (MACE). This combination of bottom-up and top-down techniques affords excellent control of nanowire etching site locations, enabling independent control of nanowire spacing, diameter and height in a single fabrication route. We demonstrate the fabrication of centimeter-scale two-dimensional gradient photonic crystals exhibiting continuously varying structural colors across the entire visible spectrum on a single silicon substrate, and the formation of tunable optical cavities supported by the VA-SiNWs, as unambiguously demonstrated through numerical simulations. Finally, Soft Nanoparticle Templating is combined with optical lithography to create hierarchical and programmable VA-SiNW patterns.

96-Channel receive-only head coil for 3 Tesla: design optimization and evaluation.

PubMed

Wiggins, Graham C; Polimeni, Jonathan R; Potthast, Andreas; Schmitt, Melanie; Alagappan, Vijay; Wald, Lawrence L

2009-09-01

The benefits and challenges of highly parallel array coils for head imaging were investigated through the development of a 3T receive-only phased-array head coil with 96 receive elements constructed on a close-fitting helmet-shaped former. We evaluated several designs for the coil elements and matching circuitry, with particular attention to sources of signal-to-noise ratio (SNR) loss, including various sources of coil loading and coupling between the array elements. The SNR and noise amplification (g-factor) in accelerated imaging were quantitatively evaluated in phantom and human imaging and compared to a 32-channel array built on an identical helmet-shaped former and to a larger commercial 12-channel head coil. The 96-channel coil provided substantial SNR gains in the distal cortex compared to the 12- and 32-channel coils. The central SNR for the 96-channel coil was similar to the 32-channel coil for optimum SNR combination and 20% lower for root-sum-of-squares combination. There was a significant reduction in the maximum g-factor for 96 channels compared to 32; for example, the 96-channel maximum g-factor was 65% of the 32-channel value for acceleration rate 4. The performance of the array is demonstrated in highly accelerated brain images.

Progress on thermobrachytherapy surface applicator for superficial tissue disease

NASA Astrophysics Data System (ADS)

Arunachalam, Kavitha; Craciunescu, Oana I.; Maccarini, Paolo F.; Schlorff, Jaime L.; Markowitz, Edward; Stauffer, Paul R.

2009-02-01

This work reports the ongoing development of a combination applicator for simultaneous heating of superficial tissue disease using a 915 MHz DCC (dual concentric conductor) array and High Dose Rate (HDR) brachytherapy delivered via an integrated conformal catheter array. The progress includes engineering design changes in the waterbolus, DCC configurations and fabrication techniques of the conformal multilayer applicator. The dosimetric impact of the thin copper DCC array is also assessed. Steady state fluid dynamics of the new waterbolus bag indicates nearly uniform flow with less than 1°C variation across a large (19×32cm) bolus. Thermometry data of the torso phantom acquired with computer controlled movement of fiberoptic temperature probes inside thermal mapping catheters indicate feasibility of real time feedback control for the DCC array. MR (magnetic resonance) scans of a torso phantom indicate that the waterbolus thickness across the treatment area is controlled by the pressure applied by the surrounding inflatable airbladder and applicator securing straps. The attenuation coefficient of the DCC array was measured as 3+/- 0.001% and 2.95+/-0.03 % using an ion chamber and OneDose dosimeters respectively. The performance of the combination applicator on patient phantoms provides valuable feedback to optimize the applicator prior use in the patient clinic.

Analyses of Genotypes and Phenotypes of Ten Chinese Patients with Wolf-Hirschhorn Syndrome by Multiplex Ligation-dependent Probe Amplification and Array Comparative Genomic Hybridization

PubMed Central

Yang, Wen-Xu; Pan, Hong; Li, Lin; Wu, Hai-Rong; Wang, Song-Tao; Bao, Xin-Hua; Jiang, Yu-Wu; Qi, Yu

2016-01-01

Background: Wolf-Hirschhorn syndrome (WHS) is a contiguous gene syndrome that is typically caused by a deletion of the distal portion of the short arm of chromosome 4. However, there are few reports about the features of Chinese WHS patients. This study aimed to characterize the clinical and molecular cytogenetic features of Chinese WHS patients using the combination of multiplex ligation-dependent probe amplification (MLPA) and array comparative genomic hybridization (array CGH). Methods: Clinical information was collected from ten patients with WHS. Genomic DNA was extracted from the peripheral blood of the patients. The deletions were analyzed by MLPA and array CGH. Results: All patients exhibited the core clinical symptoms of WHS, including severe growth delay, a Greek warrior helmet facial appearance, differing degrees of intellectual disability, and epilepsy or electroencephalogram anomalies. The 4p deletions ranged from 2.62 Mb to 17.25 Mb in size and included LETM1, WHSC1, and FGFR3. Conclusions: The combined use of MLPA and array CGH is an effective and specific means to diagnose WHS and allows for the precise identification of the breakpoints and sizes of deletions. The deletion of genes in the WHS candidate region is closely correlated with the core WHS phenotype. PMID:26960370

Transient three-dimensional thermal-hydraulic analysis of nuclear reactor fuel rod arrays: general equations and numerical scheme

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wnek, W.J.; Ramshaw, J.D.; Trapp, J.A.

1975-11-01

A mathematical model and a numerical solution scheme for thermal- hydraulic analysis of fuel rod arrays are given. The model alleviates the two major deficiencies associated with existing rod array analysis models, that of a correct transverse momentum equation and the capability of handling reversing and circulatory flows. Possible applications of the model include steady state and transient subchannel calculations as well as analysis of flows in heat exchangers, other engineering equipment, and porous media. (auth)

Deep turbulence effects mitigation with coherent combining of 21 laser beams over 7 km.

PubMed

Weyrauch, Thomas; Vorontsov, Mikhail; Mangano, Joseph; Ovchinnikov, Vladimir; Bricker, David; Polnau, Ernst; Rostov, Andrey

2016-02-15

We demonstrate coherent beam combining and adaptive mitigation of atmospheric turbulence effects over 7 km under strong scintillation conditions using a coherent fiber array laser transmitter operating in a target-in-the-loop setting. The transmitter system is composed of a densely packed array of 21 fiber collimators with integrated capabilities for piston, tip, and tilt control of the outgoing beams wavefront phases. A small cat's-eye retro reflector was used for evaluation of beam combining and turbulence compensation performance at the target plane, and to provide the feedback signal for control of piston and tip/tilt phases of the transmitted beams using the stochastic parallel gradient descent maximization of the power-in-the-bucket metric.

A simplified solar cell array modelling program

NASA Technical Reports Server (NTRS)

Hughes, R. D.

1982-01-01

As part of the energy conversion/self sufficiency efforts of DSN engineering, it was necessary to have a simplified computer model of a solar photovoltaic (PV) system. This article describes the analysis and simplifications employed in the development of a PV cell array computer model. The analysis of the incident solar radiation, steady state cell temperature and the current-voltage characteristics of a cell array are discussed. A sample cell array was modelled and the results are presented.

Interpreting aCGH-defined karyotypic changes in gliomas using copy number status, loss of heterozygosity and allelic ratios

PubMed Central

Cowell, John K; Lo, Ken C; Luce, Jesse; Hawthorn, Lesleyann

2009-01-01

We have used SNP mapping arrays to simultaneously record copy number changes, loss of heterozygosity and allele ratios (ploidy) in a series of 13 gliomas. This combined analysis has defined novel amplification events in this tumor type involving chr1:241544532-243005121 and chr18:54716681-54917277 which contain the AKT3 and ZNF532 genes respectively. The high resolution of this analysis has also identified homozygous deletions involving chr17:25600031-26490848 and Chr19:53883612-55061878. Throughout the karyotypes of these tumors, the combined analysis revealed counter intuitive relationships between copy number and LOH that requires reinterpretation of the significance of copy number gains and losses. It was not uncommon to observe copy number gains that were associated with loss of heterozygosity as well as copy number losses that were not. These events appeared to be related to ploidy status in the tumors as determined using allelic ratio calculations. Overall, this analysis of gliomas provides evidence for the need to perform more comprehensive interpretation of the CGH data beyond copy number analysis alone to evaluate the significance of individual events in the karyotypes. PMID:19818351

Digital Array Radar for Ballistic Missile Defense and Counter-Stealth Systems Analysis and Parameter Tradeoff Study

DTIC Science & Technology

2006-09-14

specified time. In general the combined vector traces an ellipse when observed in the x-y plane , as illustrated in the following figure.82 176...STANDARD Missile 3 (SM-3) launched from Aegis ships. Depth of fire with SM-3 is limited during an engagement from one 10 platform– generally one, max...will be the technical requirements for the system so that it will be capable of achieving the KPPs specified in the CDD2. Equation 3.1 is the radar

SPS phase control system performance via analytical simulation

NASA Technical Reports Server (NTRS)

Lindsey, W. C.; Kantak, A. V.; Chie, C. M.; Booth, R. W. D.

1979-01-01

A solar power satellite transmission system which incorporates automatic beam forming, steering, and phase control is discussed. The phase control concept centers around the notation of an active retrodirective phased array as a means of pointing the beam to the appropriate spot on Earth. The transmitting antenna (spacetenna) directs the high power beam so that it focuses on the ground-based receiving antenna (rectenna). A combination of analysis and computerized simulation was conducted to determine the far field performance of the reference distribution system, and the beam forming and microwave power generating systems.

Behavior of Combined Dielectric-Metallic Systems in a Charged Particle Environment

NASA Technical Reports Server (NTRS)

Gordon, W. L.; Hoffman, R. W.

1984-01-01

The charging and discharging characteristics of an electrically isolated solar array segment were studied in order to simulate discharges seen during geomagnetic substorms. A solar array segment was floated while bombarded with monoenergetic electrons at various angles of incidence. The potentials of the array surface and of the interconnects were monitored using Trek voltage probes to maintain electrical isolation. A back plate was capacitively coupled to the array to provide information on the characteristics of the transients accompanying the discharges. Several modes of discharging of the array were observed at relatively low differential and absolute potentials (a few kilovolts). A relatively slow discharge response in the array was observed, discharging over one second with currents of nanoamps. Two types of faster discharges were also seen which lasted a few hundredths of a millisecond and with currents on the order of microamps. Some results indicate an electron emission process associated with the arcs.

Formation of embedded plasmonic Ga nanoparticle arrays and their influence on GaAs photoluminescence

NASA Astrophysics Data System (ADS)

Kang, M.; Jeon, S.; Jen, T.; Lee, J.-E.; Sih, V.; Goldman, R. S.

2017-07-01

We introduce a novel approach to the seamless integration of plasmonic nanoparticle (NP) arrays into semiconductor layers and demonstrate their enhanced photoluminescence (PL) efficiency. Our approach utilizes focused ion beam-induced self-assembly of close-packed arrays of Ga NPs with tailorable NP diameters, followed by overgrowth of GaAs layers using molecular beam epitaxy. Using a combination of PL spectroscopy and electromagnetic computations, we identify a regime of Ga NP diameter and overgrown GaAs layer thickness where NP-array-enhanced absorption in GaAs leads to enhanced GaAs near-band-edge (NBE) PL efficiency, surpassing that of high-quality epitaxial GaAs layers. As the NP array depth and size are increased, the reduction in spontaneous emission rate overwhelms the NP-array-enhanced absorption, leading to a reduced NBE PL efficiency. This approach provides an opportunity to enhance the PL efficiency of a wide variety of semiconductor heterostructures.

Study of Plasma Flow Modes in Imploding Nested Arrays

NASA Astrophysics Data System (ADS)

Mitrofanov, K. N.; Aleksandrov, V. V.; Gritsuk, A. N.; Branitsky, A. V.; Frolov, I. N.; Grabovski, E. V.; Sasorov, P. V.; Ol'khovskaya, O. G.; Zaitsev, V. I.

2018-02-01

Results from experimental studies of implosion of nested wire and fiber arrays at currents of up to 4 MA at the Angara-5-1 facility are presented. Depending on the ratio between the radii of the inner and outer arrays, different modes of the plasma flow in the space between the inner and outer arrays were implemented: the sub-Alfvénic ( V r < V A ) and super-Alfvénic ( V r > V A ) modes and a mode with the formation of the transition shock wave (SW) region between the cascades. By varying the material of the outer array (tungsten wires or kapron fibers), it is shown that the plasma flow mode between the inner and outer arrays depends on the ratio between the plasma production rates ṁ in / ṁ out in the inner and outer arrays. The obtained experimental results are compared with the results of one-dimensional MHD simulation of the plasma flow between the arrays. Stable implosion of the inner array plasma was observed in experiments with combined nested arrays consisting of a fiber outer array and a tungsten inner array. The growth rates of magnetic Rayleigh-Taylor (MRT) instability in the inner array plasma at different numbers of fibers in the outer array and different ratios between the radii of the inner and outer arrays are compared. Suppression of MRT instability during the implosion of the inner array plasma results in the formation of a stable compact Z-pinch and generation of a soft X-ray pulse. A possible scenario of interaction between the plasmas of the inner and outer arrays is offered. The stability of the inner array plasma in the stage of final compression depends on the character of interaction of plasma jets from the outer array with the magnetic field of the inner array.

Chemical sensors for breath gas analysis: the latest developments at the Breath Analysis Summit 2013.

PubMed

Tisch, Ulrike; Haick, Hossam

2014-06-01

Profiling the body chemistry by means of volatile organic compounds (VOCs) in the breath opens exciting new avenues in medical diagnostics. Gas sensors could provide ideal platforms for realizing portable, hand-held breath testing devices in the near future. This review summarizes the latest developments and applications in the field of chemical sensors for diagnostic breath testing that were presented at the Breath Analysis Summit 2013 in Wallerfangen, Germany. Considerable progress has been made towards clinically applicable breath testing devices, especially by utilizing chemo-sensitive nanomaterials. Examples of several specialized breath testing applications are presented that are either based on stand-alone nanomaterial-based sensors being highly sensitive and specific to individual breath compounds over others, or on combinations of several highly specific sensors, or on experimental nanomaterial-based sensors arrays. Other interesting approaches include the adaption of a commercially available MOx-based sensor array to indirect breath testing applications, using a sample pre-concentration method, and the development of compact integrated GC-sensor systems. The recent trend towards device integration has led to the development of fully integrated prototypes of point-of-care devices. We describe and compare the performance of several prototypes that are based on different sensing technologies and evaluate their potential as low-cost and readily available next-generation medical devices.

Phased Array Imaging of Complex-Geometry Composite Components.

PubMed

Brath, Alex J; Simonetti, Francesco

2017-10-01

Progress in computational fluid dynamics and the availability of new composite materials are driving major advances in the design of aerospace engine components which now have highly complex geometries optimized to maximize system performance. However, shape complexity poses significant challenges to traditional nondestructive evaluation methods whose sensitivity and selectivity rapidly decrease as surface curvature increases. In addition, new aerospace materials typically exhibit an intricate microstructure that further complicates the inspection. In this context, an attractive solution is offered by combining ultrasonic phased array (PA) technology with immersion testing. Here, the water column formed between the complex surface of the component and the flat face of a linear or matrix array probe ensures ideal acoustic coupling between the array and the component as the probe is continuously scanned to form a volumetric rendering of the part. While the immersion configuration is desirable for practical testing, the interpretation of the measured ultrasonic signals for image formation is complicated by reflection and refraction effects that occur at the water-component interface. To account for refraction, the geometry of the interface must first be reconstructed from the reflected signals and subsequently used to compute suitable delay laws to focus inside the component. These calculations are based on ray theory and can be computationally intensive. Moreover, strong reflections from the interface can lead to a thick dead zone beneath the surface of the component which limits sensitivity to shallow subsurface defects. This paper presents a general approach that combines advanced computing for rapid ray tracing in anisotropic media with a 256-channel parallel array architecture. The full-volume inspection of complex-shape components is enabled through the combination of both reflected and transmitted signals through the part using a pair of arrays held in a yoke configuration. Experimental results are provided for specimens of increasing complexity relevant to aerospace applications such as fan blades. It is shown that PA technology can provide a robust solution to detect a variety of defects including porosity and waviness in composite parts.

Coil combination for receive array spectroscopy: Are data-driven methods superior to methods using computed field maps?

PubMed

Rodgers, Christopher T; Robson, Matthew D

2016-02-01

Combining spectra from receive arrays, particularly X-nuclear spectra with low signal-to-noise ratios (SNRs), is challenging. We test whether data-driven combination methods are better than using computed coil sensitivities. Several combination algorithms are recast into the notation of Roemer's classic formula, showing that they differ primarily in their estimation of coil receive sensitivities. This viewpoint reveals two extensions of the whitened singular-value decomposition (WSVD) algorithm, using temporal or temporal + spatial apodization to improve the coil sensitivities, and thus the combined spectral SNR. Radiofrequency fields from an array were simulated and used to make synthetic spectra. These were combined with 10 algorithms. The combined spectra were then assessed in terms of their SNR. Validation used phantoms and cardiac (31) P spectra from five subjects at 3T. Combined spectral SNRs from simulations, phantoms, and humans showed the same trends. In phantoms, the combined SNR using computed coil sensitivities was lower than with WSVD combination whenever the WSVD SNR was >14 (or >11 with temporal apodization, or >9 with temporal + spatial apodization). These new apodized WSVD methods gave higher SNRs than other data-driven methods. In the human torso, at frequencies ≥49 MHz, data-driven combination is preferable to using computed coil sensitivities. Magn Reson, 2015. © 2015 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Magn Reson Med 75:473-487, 2016. © 2015 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. © 2015 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine.

The Atacama Cosmology Telescope: The Polarization-Sensitive ACTPol Instrument

NASA Technical Reports Server (NTRS)

Thornton, R. J.; Ade, P. A. R.; Aiola, S.; Angile, F. E.; Amiri, M.; Beall, J. A.; Becker, D. T.; Cho, H.-M.; Choi, S. K.; Corlies, P.;

The Atacama Cosmology Telescope: The Polarization-sensitive ACTPol Instrument

NASA Astrophysics Data System (ADS)

Thornton, R. J.; Ade, P. A. R.; Aiola, S.; Angilè, F. E.; Amiri, M.; Beall, J. A.; Becker, D. T.; Cho, H.-M.; Choi, S. K.; Corlies, P.; Coughlin, K. P.; Datta, R.; Devlin, M. J.; Dicker, S. R.; Dünner, R.; Fowler, J. W.; Fox, A. E.; Gallardo, P. A.; Gao, J.; Grace, E.; Halpern, M.; Hasselfield, M.; Henderson, S. W.; Hilton, G. C.; Hincks, A. D.; Ho, S. P.; Hubmayr, J.; Irwin, K. D.; Klein, J.; Koopman, B.; Li, Dale; Louis, T.; Lungu, M.; Maurin, L.; McMahon, J.; Munson, C. D.; Naess, S.; Nati, F.; Newburgh, L.; Nibarger, J.; Niemack, M. D.; Niraula, P.; Nolta, M. R.; Page, L. A.; Pappas, C. G.; Schillaci, A.; Schmitt, B. L.; Sehgal, N.; Sievers, J. L.; Simon, S. M.; Staggs, S. T.; Tucker, C.; Uehara, M.; van Lanen, J.; Ward, J. T.; Wollack, E. J.

2016-12-01

The Atacama Cosmology Telescope (ACT) makes high angular resolution measurements of anisotropies in the Cosmic Microwave Background (CMB) at millimeter wavelengths. We describe ACTPol, an upgraded receiver for ACT, which uses feedhorn-coupled, polarization-sensitive detector arrays, a 3° field of view, 100 mK cryogenics with continuous cooling, and meta material antireflection coatings. ACTPol comprises three arrays with separate cryogenic optics: two arrays at a central frequency of 148 GHz and one array operating simultaneously at both 97 GHz and 148 GHz. The combined instrument sensitivity, angular resolution, and sky coverage are optimized for measuring angular power spectra, clusters via the thermal Sunyaev–Zel’dovich (SZ) and kinetic SZ signals, and CMB lensing due to large-scale structure. The receiver was commissioned with its first 148 GHz array in 2013, observed with both 148 GHz arrays in 2014, and has recently completed its first full season of operations with the full suite of three arrays. This paper provides an overview of the design and initial performance of the receiver and related systems.

Flexible retinal electrode array

DOEpatents

Okandan, Murat [Albuquerque, NM; Wessendorf, Kurt O [Albuquerque, NM; Christenson, Todd R [Albuquerque, NM

2006-10-24

An electrode array which has applications for neural stimulation and sensing. The electrode array can include a large number of electrodes each of which is flexibly attached to a common substrate using a plurality of springs to allow the electrodes to move independently. The electrode array can be formed from a combination of bulk and surface micromachining, with electrode tips that can include an electroplated metal (e.g. platinum, iridium, gold or titanium) or a metal oxide (e.g. iridium oxide) for biocompatibility. The electrode array can be used to form a part of a neural prosthesis, and is particularly well adapted for use in an implantable retinal prosthesis where the electrodes can be tailored to provide a uniform gentle contact pressure with optional sensing of this contact pressure at one or more of the electrodes.

Optical phased array configuration for an extremely large telescope.

PubMed

Meinel, Aden Baker; Meinel, Marjorie Pettit

2004-01-20

Extremely large telescopes are currently under consideration by several groups in several countries. Extrapolation of current technology up to 30 m indicates a cost of over dollars 1 billion. Innovative concepts are being explored to find significant cost reductions. We explore the concept of an Optical Phased Array (OPA) telescope. Each element of the OPA is a separate Cassegrain telescope. Collimated beams from the array are sent via an associated set of delay lines to a central beam combiner. This array of small telescope elements offers the possibility of starting with a low-cost array of a few rings of elements, adding structure and additional Cass elements until the desired diameter telescope is attained. We address the salient features of such an extremely large telescope and cost elements relative to more conventional options.

Plasmonic Ag nanostructures on thin substrates for enhanced energy harvesting

NASA Astrophysics Data System (ADS)

Osgood, R. M.; Giardini, S. A.; Carlson, J. B.; Gear, C.; Diest, K.; Rothschild, M.; Fernandes, G. E.; Xu, J.; Kooi, S.; Periasamy, P.; O'Hayre, R.; Parilla, P.; Berry, J.; Ginley, D.

2013-09-01

Nanoparticles and nanostructures with plasmonic resonances are currently being employed to enhance the efficiency of solar cells. Ag stripe arrays have been shown theoretically to enhance the short-circuit current of thin silicon layers. Such Ag stripes are combined with 200 nm long and 60 nm wide "teeth", which act as nanoantennas, and form vertical rectifying metal-insulator-metal (MIM) nanostructures on metallic substrates coated with thin oxides, such as Nb/NbOx films. We characterize experimentally and theoretically the visible and near-infrared spectra of these "stripeteeth" arrays, which act as microantenna arrays for energy harvesting and detection, on silicon substrates. Modeling the stripe-teeth arrays predicts a substantial net a.c. voltage across the MIM diode, even when the stripe-teeth microrectenna arrays are illuminated at normal incidence.

A New All Solid State Approach to Gaseous Pollutant Detection

NASA Technical Reports Server (NTRS)

Brown, V.; Tamstorf, K.

1971-01-01

Recent efforts in our laboratories have concentrated on the development of an all solid state gas sensor, by combining solid electrolyte (ion exchange membrane) technology with advanced thin film deposition processes. With the proper bias magnitude and polarity these miniature electro-chemical,cells show remarkable current responses for many common pollution gases. Current activity is now focused on complementing a multiple array (matrix) of these solid state sensors, with a digital electronic scanner device possessing "scan-compare-identify-alarm: capability. This innovative approach to multi-component pollutant gas analysis may indeed be the advanced prototype for the "third generation" class of pollution analysis instrumentation so urgently needed in the decade ahead.

Application of fuzzy logic in multicomponent analysis by optodes.

PubMed

Wollenweber, M; Polster, J; Becker, T; Schmidt, H L

1997-01-01

Fuzzy logic can be a useful tool for the determination of substrate concentrations applying optode arrays in combination with flow injection analysis, UV-VIS spectroscopy and kinetics. The transient diffuse reflectance spectra in the visible wavelength region from four optodes were evaluated to carry out the simultaneous determination of artificial mixtures of ampicillin and penicillin. The discrimination of the samples was achieved by changing the composition of the receptor gel and working pH. Different algorithms of pre-processing were applied on the data to reduce the spectral information to a few analytic-specific variables. These variables were used to develop the fuzzy model. After calibration the model was validated by an independent test data set.

PSHFT - COMPUTERIZED LIFE AND RELIABILITY MODELLING FOR TURBOPROP TRANSMISSIONS

NASA Technical Reports Server (NTRS)

Savage, M.

1994-01-01

The computer program PSHFT calculates the life of a variety of aircraft transmissions. A generalized life and reliability model is presented for turboprop and parallel shaft geared prop-fan aircraft transmissions. The transmission life and reliability model is a combination of the individual reliability models for all the bearings and gears in the main load paths. The bearing and gear reliability models are based on the statistical two parameter Weibull failure distribution method and classical fatigue theories. The computer program developed to calculate the transmission model is modular. In its present form, the program can analyze five different transmissions arrangements. Moreover, the program can be easily modified to include additional transmission arrangements. PSHFT uses the properties of a common block two-dimensional array to separate the component and transmission property values from the analysis subroutines. The rows correspond to specific components with the first row containing the values for the entire transmission. Columns contain the values for specific properties. Since the subroutines (which determine the transmission life and dynamic capacity) interface solely with this property array, they are separated from any specific transmission configuration. The system analysis subroutines work in an identical manner for all transmission configurations considered. Thus, other configurations can be added to the program by simply adding component property determination subroutines. PSHFT consists of a main program, a series of configuration specific subroutines, generic component property analysis subroutines, systems analysis subroutines, and a common block. The main program selects the routines to be used in the analysis and sequences their operation. The series of configuration specific subroutines input the configuration data, perform the component force and life analyses (with the help of the generic component property analysis subroutines), fill the property array, call up the system analysis routines, and finally print out the analysis results for the system and components. PSHFT is written in FORTRAN 77 and compiled on a MicroSoft FORTRAN compiler. The program will run on an IBM PC AT compatible with at least 104k bytes of memory. The program was developed in 1988.

High-performance, flexible, deployable array development for space applications

NASA Technical Reports Server (NTRS)

Gehling, Russell N.; Armstrong, Joseph H.; Misra, Mohan S.

1994-01-01

Flexible, deployable arrays are an attractive alternative to conventional solar arrays for near-term and future space power applications, particularly due to their potential for high specific power and low storage volume. Combined with low-cost flexible thin-film photovoltaics, these arrays have the potential to become an enabling or an enhancing technology for many missions. In order to expedite the acceptance of thin-film photovoltaics for space applications, however, parallel development of flexible photovoltaics and the corresponding deployable structure is essential. Many innovative technologies must be incorporated in these arrays to ensure a significant performance increase over conventional technologies. For example, innovative mechanisms which employ shape memory alloys for storage latches, deployment mechanisms, and array positioning gimbals can be incorporated into flexible array design with significant improvement in the areas of cost, weight, and reliability. This paper discusses recent activities at Martin Marietta regarding the development of flexible, deployable solar array technology. Particular emphasis is placed on the novel use of shape memory alloys for lightweight deployment elements to improve the overall specific power of the array. Array performance projections with flexible thin-film copper-indium-diselenide (CIS) are presented, and government-sponsored solar array programs recently initiated at Martin Marietta through NASA and Air Force Phillips Laboratory are discussed.

Servo scanning 3D micro EDM for array micro cavities using on-machine fabricated tool electrodes

NASA Astrophysics Data System (ADS)

Tong, Hao; Li, Yong; Zhang, Long

2018-02-01

Array micro cavities are useful in many fields including in micro molds, optical devices, biochips and so on. Array servo scanning micro electro discharge machining (EDM), using array micro electrodes with simple cross-sectional shape, has the advantage of machining complex 3D micro cavities in batches. In this paper, the machining errors caused by offline-fabricated array micro electrodes are analyzed in particular, and then a machining process of array servo scanning micro EDM is proposed by using on-machine fabricated array micro electrodes. The array micro electrodes are fabricated on-machine by combined procedures including wire electro discharge grinding, array reverse copying and electrode end trimming. Nine-array tool electrodes with Φ80 µm diameter and 600 µm length are obtained. Furthermore, the proposed process is verified by several machining experiments for achieving nine-array hexagonal micro cavities with top side length of 300 µm, bottom side length of 150 µm, and depth of 112 µm or 120 µm. In the experiments, a chip hump accumulates on the electrode tips like the built-up edge in mechanical machining under the conditions of brass workpieces, copper electrodes and the dielectric of deionized water. The accumulated hump can be avoided by replacing the water dielectric by an oil dielectric.

Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant

PubMed Central

Zhao, Ji-Hui; Zhang, Qi-Bo; Liu, Bao; Piao, Xiang-Hua; Yan, Yu-Lu; Hu, Xiao-Ge; Zhou, Kuan; Zhang, Yong-Tai; Feng, Nian-Ping

2017-01-01

Purpose To enhance the immunogenicity of the model subunit vaccine, ovalbumin (OVA) was combined with platycodin (PD), a saponin adjuvant. To reduce the toxicity of PD, OVA, and adjuvant were loaded together into liposomes before being incorporated into a dissolving microneedle array. Methods OVA- and PD-loaded liposomes (OVA-PD-Lipos) were prepared using the film dispersion method. Their uptake behavior, toxicity to mouse bone marrow dendritic cells (BMDCs), and hemolytic activity to rabbit red blood cells (RBCs) were evaluated. The OVA-PD-Lipos were incorporated into a dissolving microneedle array. The chemical stability of OVA and the physical stability of OVA-PD-Lipos in microneedle arrays were investigated. The immune response of Institute of Cancer Research mice and potential skin irritation reaction of rabbits to OVA-PD-Lipos-MNs were evaluated. Results The uptake of OVA by mouse BMDCs was greatly enhanced when OVA was prepared as OVA-PD-Lipos, and in this form, the toxicity of PD was dramatically reduced. OVA was chemically stable as OVA-PD-Lipos, when OVA-PD-Lipos was incorporated into a dissolving microneedle array. Institute of Cancer Research mice treated with OVA-PD-Lipos-MNs showed a significantly enhanced immune response. PD combined with OVA elicited a balanced Th1 and Th2 humoral immune response in mice, with minimal irritation in rabbit skin. Conclusion The dissolving microneedle array-based system is a promising delivery vehicle for subunit vaccine and its adjuvant. PMID:28740383

Generalized enhanced suffix array construction in external memory.

PubMed

Louza, Felipe A; Telles, Guilherme P; Hoffmann, Steve; Ciferri, Cristina D A

2017-01-01

Suffix arrays, augmented by additional data structures, allow solving efficiently many string processing problems. The external memory construction of the generalized suffix array for a string collection is a fundamental task when the size of the input collection or the data structure exceeds the available internal memory. In this article we present and analyze [Formula: see text] [introduced in CPM (External memory generalized suffix and [Formula: see text] arrays construction. In: Proceedings of CPM. pp 201-10, 2013)], the first external memory algorithm to construct generalized suffix arrays augmented with the longest common prefix array for a string collection. Our algorithm relies on a combination of buffers, induced sorting and a heap to avoid direct string comparisons. We performed experiments that covered different aspects of our algorithm, including running time, efficiency, external memory access, internal phases and the influence of different optimization strategies. On real datasets of size up to 24 GB and using 2 GB of internal memory, [Formula: see text] showed a competitive performance when compared to [Formula: see text] and [Formula: see text], which are efficient algorithms for a single string according to the related literature. We also show the effect of disk caching managed by the operating system on our algorithm. The proposed algorithm was validated through performance tests using real datasets from different domains, in various combinations, and showed a competitive performance. Our algorithm can also construct the generalized Burrows-Wheeler transform of a string collection with no additional cost except by the output time.

Cu-PDC-bpa solid coordination frameworks (PDC=2,5-pyrindinedicarboxylate; bpa=1,2-DI(4-pyridil)ethane)): 2D and 3D structural flexibility producing a 3-c herringbone array next to ideal

NASA Astrophysics Data System (ADS)

Llano-Tomé, Francisco; Bazán, Begoña; Urtiaga, Miren-Karmele; Barandika, Gotzone; Antonia Señarís-Rodríguez, M.; Sánchez-Andújar, Manuel; Arriortua, María-Isabel

2015-10-01

Combination of polycarboxylate anions and dipyridyl ligands is an effective strategy to produce solid coordination frameworks (SCF) which are crystalline materials based on connections between metal ions through organic ligands. In this context, this work is focused on two novel CuII-based SCFs exhibiting PDC (2,5-pyridinedicarboxylate) and bpa (1,2-di(4-pyridyl)ethane), being the first structures reported in literature containing both ligands. Chemical formula are [Cu2[(PDC)2(bpa)(H2O)2]·3H2O·DMF (1), and [Cu2(PDC)2(bpa)(H2O)2]·7H2O (2), where DMF is dimethylformamide. Compounds 1 and 2 have been characterized by means of X-ray diffraction (XRD), infrared spectroscopy (IR), thermogravimetric (TG) analysis, differential thermal analysis (DTA) and dielectric measurements. The crystallographic analysis revealed that compounds 1 and 2 can be described as herringbone-type layers formed by helicoidal Cu-PDC-Cu chains connected through bpa ligands. Solvent molecules are crystallized between the layers, providing the inter-layer connections through hydrogen bonds. Differences between both compounds are attributable to the flexibility of bpa (in 2D) as well as to the 3D packing of the layers which is solvent dependent. This fact results in the fact that compound 2 is the most regular 3-c herringbone array reported so far. The structural dynamism of these networks is responsible for the crystalline to-amorphous to-crystalline (CAC) transformation from compound 1 to compound 2. Crystallochemical features for both compounds have also been studied and compared to similar 3-connected herringbone-arrays.

Fast batch injection analysis of H(2)O(2) using an array of Pt-modified gold microelectrodes obtained from split electronic chips.

PubMed

Pacheco, Bruno D; Valério, Jaqueline; Angnes, Lúcio; Pedrotti, Jairo J

2011-06-24

A fast and robust analytical method for amperometric determination of hydrogen peroxide (H(2)O(2)) based on batch injection analysis (BIA) on an array of gold microelectrodes modified with platinum is proposed. The gold microelectrode array (n=14) was obtained from electronic chips developed for surface mounted device technology (SMD), whose size offers advantages to adapt them in batch cells. The effect of the dispensing rate, volume injected, distance between the platinum microelectrodes and the pipette tip, as well as the volume of solution in the cell on the analytical response were evaluated. The method allows the H(2)O(2) amperometric determination in the concentration range from 0.8 μmolL(-1) to 100 μmolL(-1). The analytical frequency can attain 300 determinations per hour and the detection limit was estimated in 0.34 μmolL(-1) (3σ). The anodic current peaks obtained after a series of 23 successive injections of 50 μL of 25 μmolL(-1) H(2)O(2) showed an RSD<0.9%. To ensure the good selectivity to detect H(2)O(2), its determination was performed in a differential mode, with selective destruction of the H(2)O(2) with catalase in 10 mmolL(-1) phosphate buffer solution. Practical application of the analytical procedure involved H(2)O(2) determination in rainwater of São Paulo City. A comparison of the results obtained by the proposed amperometric method with another one which combines flow injection analysis (FIA) with spectrophotometric detection showed good agreement. Copyright © 2011 Elsevier B.V. All rights reserved.

A 7T spine array based on electric dipole transmitters.

PubMed

Duan, Qi; Nair, Govind; Gudino, Natalia; de Zwart, Jacco A; van Gelderen, Peter; Murphy-Boesch, Joe; Reich, Daniel S; Duyn, Jeff H; Merkle, Hellmut

2015-10-01

The goal of this study was to explore the feasibility of using an array of electric dipole antennas for RF transmission in spine MRI at high fields. A two-channel transmit array based on an electric dipole design was quantitatively optimized for 7T spine imaging and integrated with a receive array combining eight loop coils. Using B1+ mapping, the transmit efficiency of the dipole array was compared with a design using quadrature loop pairs. The radiofrequency energy deposition for each array was measured using a home-built dielectric phantom and MR thermometry. The performance of the proposed array was qualitatively demonstrated in human studies. The results indicate dramatically improved transmit efficiency for the dipole design compared with the loop excitation. A gain of up to 76% was achieved within the spinal region. For imaging of the spine, electric dipole-based transmitters provide an attractive alternative to the traditional loop-based design. Easy integration with existing receive array technology facilitates practical use at high fields. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

Flat-plate photovoltaic array design optimization

NASA Technical Reports Server (NTRS)

Ross, R. G., Jr.

1980-01-01

An analysis is presented which integrates the results of specific studies in the areas of photovoltaic structural design optimization, optimization of array series/parallel circuit design, thermal design optimization, and optimization of environmental protection features. The analysis is based on minimizing the total photovoltaic system life-cycle energy cost including repair and replacement of failed cells and modules. This approach is shown to be a useful technique for array optimization, particularly when time-dependent parameters such as array degradation and maintenance are involved.

BeadArray Expression Analysis Using Bioconductor

PubMed Central

Ritchie, Matthew E.; Dunning, Mark J.; Smith, Mike L.; Shi, Wei; Lynch, Andy G.

2011-01-01

Illumina whole-genome expression BeadArrays are a popular choice in gene profiling studies. Aside from the vendor-provided software tools for analyzing BeadArray expression data (GenomeStudio/BeadStudio), there exists a comprehensive set of open-source analysis tools in the Bioconductor project, many of which have been tailored to exploit the unique properties of this platform. In this article, we explore a number of these software packages and demonstrate how to perform a complete analysis of BeadArray data in various formats. The key steps of importing data, performing quality assessments, preprocessing, and annotation in the common setting of assessing differential expression in designed experiments will be covered. PMID:22144879

Rapid Analysis, Self-Calibrating Array for Air Monitoring

NASA Technical Reports Server (NTRS)

Homer, Margie L.; Shevade, Abhijit V.; Lara, Liana; Huerta, Ramon; Vergara, Alexander; Muezzinoglua, Mehmet K.

2012-01-01

Human space missions have critical needs for monitoring and control for life support systems. These systems have monitoring needs that include feedback for closed loop processes and quality control for environmental factors. Sensors and monitoring technologies assure that the air environment and water supply for the astronaut crew habitat fall within acceptable limits, and that the life support system is functioning properly and efficiently. The longer the flight duration and the more distant the destination, the more critical it becomes to have carefully monitored and automated control systems for life support. Past experiments with the JPL ENose have demonstrated a lifetime of the sensor array, with the software, of around 18 months. The lifetime of the calibration, for some analytes, was as long as 24 months. We are working on a sensor array and new algorithms that will include sensor response time in the analysis. The preliminary array analysis for two analytes shows that the analysis time, of an event, can be dropped from 45 minutes to less than10 minutes and array training time can be cut substantially. We will describe the lifetime testing of an array and show lifetime data on individual sensors. This progress will lead to more rapid identification of analytes, and faster training time of the array.

Defining ray sets for the analysis of lenslet-based optical systems including plenoptic cameras and Shack-Hartmann wavefront sensors

NASA Astrophysics Data System (ADS)

Moore, Lori

Plenoptic cameras and Shack-Hartmann wavefront sensors are lenslet-based optical systems that do not form a conventional image. The addition of a lens array into these systems allows for the aberrations generated by the combination of the object and the optical components located prior to the lens array to be measured or corrected with post-processing. This dissertation provides a ray selection method to determine the rays that pass through each lenslet in a lenslet-based system. This first-order, ray trace method is developed for any lenslet-based system with a well-defined fore optic, where in this dissertation the fore optic is all of the optical components located prior to the lens array. For example, in a plenoptic camera the fore optic is a standard camera lens. Because a lens array at any location after the exit pupil of the fore optic is considered in this analysis, it is applicable to both plenoptic cameras and Shack-Hartmann wavefront sensors. Only a generic, unaberrated fore optic is considered, but this dissertation establishes a framework for considering the effect of an aberrated fore optic in lenslet-based systems. The rays from the fore optic that pass through a lenslet placed at any location after the fore optic are determined. This collection of rays is reduced to three rays that describe the entire lenslet ray set. The lenslet ray set is determined at the object, image, and pupil planes of the fore optic. The consideration of the apertures that define the lenslet ray set for an on-axis lenslet leads to three classes of lenslet-based systems. Vignetting of the lenslet rays is considered for off-axis lenslets. Finally, the lenslet ray set is normalized into terms similar to the field and aperture vector used to describe the aberrated wavefront of the fore optic. The analysis in this dissertation is complementary to other first-order models that have been developed for a specific plenoptic camera layout or Shack-Hartmann wavefront sensor application. This general analysis determines the location where the rays of each lenslet pass through the fore optic establishing a framework to consider the effect of an aberrated fore optic in a future analysis.

Cu-PDC-bpa solid coordination frameworks (PDC=2,5-pyrindinedicarboxylate; bpa=1,2-DI(4-pyridil)ethane)): 2D and 3D structural flexibility producing a 3-c herringbone array next to ideal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Llano-Tomé, Francisco, E-mail: francisco.llano@ehu.eus; Bazán, Begoña, E-mail: bego.bazan@ehu.eus; BCMaterials Parque Tecnológico de Zamudio, Ibaizabal Bidea, Edificio 500–Planta 1, 48160 Derio

Combination of polycarboxylate anions and dipyridyl ligands is an effective strategy to produce solid coordination frameworks (SCF) which are crystalline materials based on connections between metal ions through organic ligands. In this context, this work is focused on two novel Cu{sup II}-based SCFs exhibiting PDC (2,5-pyridinedicarboxylate) and bpa (1,2-di(4-pyridyl)ethane), being the first structures reported in literature containing both ligands. Chemical formula are [Cu{sub 2}[(PDC){sub 2}(bpa)(H{sub 2}O){sub 2}]·3H{sub 2}O·DMF (1), and [Cu{sub 2}(PDC){sub 2}(bpa)(H{sub 2}O){sub 2}]·7H{sub 2}O (2), where DMF is dimethylformamide. Compounds 1 and 2 have been characterized by means of X-ray diffraction (XRD), infrared spectroscopy (IR), thermogravimetric (TG) analysis,more » differential thermal analysis (DTA) and dielectric measurements. The crystallographic analysis revealed that compounds 1 and 2 can be described as herringbone-type layers formed by helicoidal Cu-PDC-Cu chains connected through bpa ligands. Solvent molecules are crystallized between the layers, providing the inter-layer connections through hydrogen bonds. Differences between both compounds are attributable to the flexibility of bpa (in 2D) as well as to the 3D packing of the layers which is solvent dependent. This fact results in the fact that compound 2 is the most regular 3-c herringbone array reported so far. The structural dynamism of these networks is responsible for the crystalline to-amorphous to-crystalline (CAC) transformation from compound 1 to compound 2. Crystallochemical features for both compounds have also been studied and compared to similar 3-connected herringbone-arrays. - Graphical abstract: Cu-PDC-bpa 3-c herringbone arrays. - Highlights: • The most ideal herringbone array reported so far is a Cu-PDC-bpa SCF. • Conformational freedom of bpa results in 2D and 3D flexibility of the SCFs. • The flexibility of the SCFs is related to a phase transformation. • Dielectric measurements confirm the mobility of guest solvent molecules.« less

Integration of Fiber-Optic Sensor Arrays into a Multi-Modal Tactile Sensor Processing System for Robotic End-Effectors

PubMed Central

Kampmann, Peter; Kirchner, Frank

2014-01-01

With the increasing complexity of robotic missions and the development towards long-term autonomous systems, the need for multi-modal sensing of the environment increases. Until now, the use of tactile sensor systems has been mostly based on sensing one modality of forces in the robotic end-effector. The use of a multi-modal tactile sensory system is motivated, which combines static and dynamic force sensor arrays together with an absolute force measurement system. This publication is focused on the development of a compact sensor interface for a fiber-optic sensor array, as optic measurement principles tend to have a bulky interface. Mechanical, electrical and software approaches are combined to realize an integrated structure that provides decentralized data pre-processing of the tactile measurements. Local behaviors are implemented using this setup to show the effectiveness of this approach. PMID:24743158

Determining SAFOD area microearthquake locations solely with the Pilot Hole seismic array data

NASA Astrophysics Data System (ADS)

Oye, Volker; Chavarria, J. Andres; Malin, Peter E.

2004-05-01

In August 2002, an array of 32 three-component geophones was installed in the San Andreas Fault Observatory at Depth (SAFOD) Pilot Hole (PH) at Parkfield, CA. As an independent test of surface-observation-based microearthquake locations, we have located such events using only data recorded on the PH array. We then compared these locations with locations from a combined set of PH and Parkfield High Resolution Seismic Network (HRSN) observations. We determined the uncertainties in the locations as they relate to errors in the travel time picks and the velocity model by the bootstrap method. Based on the PH and combined locations, we find that the ``C2'' cluster to the northeast of the PH has the smallest location uncertainties. Events in this cluster also have the most similar waveforms and largest magnitudes. This confirms earlier suggestions that the C2 cluster is a promising target for the SAFOD Main Hole.

Protein recognition by a pattern-generating fluorescent molecular probe.

PubMed

Pode, Zohar; Peri-Naor, Ronny; Georgeson, Joseph M; Ilani, Tal; Kiss, Vladimir; Unger, Tamar; Markus, Barak; Barr, Haim M; Motiei, Leila; Margulies, David

2017-12-01

Fluorescent molecular probes have become valuable tools in protein research; however, the current methods for using these probes are less suitable for analysing specific populations of proteins in their native environment. In this study, we address this gap by developing a unimolecular fluorescent probe that combines the properties of small-molecule-based probes and cross-reactive sensor arrays (the so-called chemical 'noses/tongues'). On the one hand, the probe can detect different proteins by generating unique identification (ID) patterns, akin to cross-reactive arrays. On the other hand, its unimolecular scaffold and selective binding enable this ID-generating probe to identify combinations of specific protein families within complex mixtures and to discriminate among isoforms in living cells, where macroscopic arrays cannot access. The ability to recycle the molecular device and use it to track several binding interactions simultaneously further demonstrates how this approach could expand the fluorescent toolbox currently used to detect and image proteins.

DEATH-STAR: Silicon and photovoltaic fission fragment detector arrays for light-ion induced fission correlation studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Koglin, J. D.; Burke, J. T.; Fisher, S. E.

Here, the Direct Excitation Angular Tracking pHotovoltaic-Silicon Telescope ARray (DEATH-STAR) combines a series of 12 silicon detectors in a ΔE–E configuration for charged particle identification with a large-area array of 56 photovoltaic (solar) cells for detection of fission fragments. The combination of many scattering angles and fission fragment detectors allows for an angular-resolved tool to study reaction cross sections using the surrogate method, anisotropic fission distributions, and angular momentum transfers through stripping, transfer, inelastic scattering, and other direct nuclear reactions. The unique photovoltaic detectors efficiently detect fission fragments while being insensitive to light ions and have a timing resolution ofmore » 15.63±0.37 ns. Alpha particles are detected with a resolution of 35.5 keV 1σ at 7.9 MeV. Measured fission fragment angular distributions are also presented.« less